WorldWideScience

Sample records for wallstent shortening resulting

  1. Shortening of Wallstent RP during carotid artery stenting requires appropriate stent placement.

    Science.gov (United States)

    Aikawa, Hiroshi; Nagata, Shun-ichi; Onizuka, Masanari; Tsutsumi, Masanori; Iko, Minoru; Kodama, Tomonobu; Nii, Kouhei; Matsubara, Shuko; Etou, Hosei; Go, Yoshinori; Kazekawa, Kiyoshi

    2008-06-01

    Changes in the location and length of the Wallstent RP during carotid artery stenting (CAS) were evaluated using intraoperative videos of 28 patients with carotid artery stenosis who underwent CAS with a 10/20 mm Wallstent RP to determine the appropriate stent placement. The stent was deployed after its midpoint was positioned over a virtual center line, the perpendicular line which crossed the most stenotic point of the lesion on the road mapping image. The length of the stenotic lesion, the changes in the locations of the distal and proximal ends of the stent, and the changes in stent length were examined. The distal end of the stent moved a maximum of 6.1 mm toward the proximal side to a point 19.9 mm from the virtual center line. The proximal end moved a maximum of 11.3 mm toward the distal side to a point 14.7 mm from the virtual center line. The stent length ranged from 37.7 to 44.5 mm (mean 41.2 mm). The 10/20 mm Wallstent RP placed by our technique covers the entire lesion with no less than 5.7 mm of margin over the segment distal to the lesion in patients with stenotic segments shorter than 29.4 mm.

  2. Relative risk analysis of angiographic predictors of restenosis within the coronary Wallstent

    NARCIS (Netherlands)

    B.H. Strauss (Bradley); P.W.J.C. Serruys (Patrick); I.K. de Scheerder (Ivan); J.G.P. Tijssen (Jan); M.E. Bertrand (Michel); J. Puel (Jacques); B. Meier (Bernard); U. Kaufmann; J.C. Stauffer; A.F. Rickards (Anthony); U. Sigwart (Ulrich)

    1991-01-01

    textabstractBACKGROUND. Late angiographic narrowing has been observed following coronary implantation of the Wallstent. To identify the angiographic variables that predict restenosis within the stented segment, a retrospective study of data from the European Wallstent core laboratory was performed.

  3. Comparison of a new stent and Wallstent for transjugular intrahepatic portosystemic shunt in a porcine model

    Institute of Scientific and Technical Information of China (English)

    Gao Jun Teng; Michael A. Bettmann; P. Jack Hoopes; Li Yang

    2001-01-01

    AIM To evaluate a new balloon-expandablestainless steel stent (Cordis stent ) in atransjugular intrahepatic portosystemic shunt(TIPS) porcine model and compared withWallstent.METHODS TIPS was performed in 26 normaldomestic pigs weighing 20 kg-30 kg using aCordis stent or Wallstent (13 pigs in each stent).All pigs were sacrificed at the 14th day afterTIPS. The stent deployment delivery system,stent patency, and stent recoil after placementwere evaluated. Proliferative response inrepresentative histological sections from thecenter, hepatic and portal regions of the twostent designs were quantified.RESULTS The shunt was widely patent in 4pigs in the Cordis stent group (4/12, prematuredead in 1 pig), and in 5 pigs in the Wallstentgroup (5/13). All remaining stents of bothdesigns were occluded or stenotic. The meanquantified proliferation including thickness ofthe proliferation and the ratio of proliferation:total area in three assayed regions in Cordisstent and Wallstent was 2.18 mm:2.00 mm, and59.18 mm2: 51.66 mm2, respectively (P >0.05).The delivery system and mechanical propertiesof the Cordis stent fuctioned well.CONCLUSION The new Cordis stent isappropriate for TIPS procedure.

  4. The Oblique Metaphyseal Shortening Osteotomy of the Distal Ulna: Surgical Technique and Results of Ten Patients.

    Science.gov (United States)

    Benis, Szabolcs; Goubau, Jean F; Mermuys, Koen; Van Hoonacker, Petrus; Berghs, Bart; Kerckhove, Diederick; Vanmierlo, Bert

    2017-02-01

    Background Ulnocarpal abutment is a common condition following distal radius fractures. There are different surgical methods of treatment for this pathology: open and arthroscopic wafer procedure or an ulnar shortening osteotomy. We describe an oblique metaphyseal shortening osteotomy of the distal ulna using two cannulated headless compression screws. We report the results of 10 patients treated with this method. Materials and Methods Out of 17 patients, 10 could be reviewed retrospectively for this study. Patient-rated outcomes were measured using the VAS (visual analogue scale) for pain, PRWHE (patient-rated wrist and hand evaluation) survey, and Quick-DASH (disability of arm, shoulder and hand) survey for functional outcomes. At the review we measured the range of motion (ROM) of the wrist (extension and flexion, ulnar and radial deviation, pronation and supination). Grip strength, pronation, and supination strength of the forearm was measured using a calibrated hydraulic dynamometer. ROM and strength of the affected wrist was compared with ROM and strength of the unaffected wrist. Surgical Procedure Oblique long metaphyseal osteotomy of the distal ulna (from proximal-ulnar to distal-radial), fixed with two cannulated headless compression screws. Results The average postoperative VAS score for pain was 23.71 (standard deviation [SD] of 30.41). The average postoperative PRWHE score was 32.55 (SD of 26.28). The average postoperative Quick-DASH score was 28.65 (SD of 27.21). The majority of patients had a comparable ROM and strength between the operated side and the non-operated side. Conclusion This surgical technique has the advantage of reducing the amount of hardware and to decrease the potential hinder caused by it on medium term. Moreover, the incision remains smaller, and the anatomic metaphyseal localization of the osteotomy potentially allows a better and rapid healing.

  5. Comparison between esophageal Wallstent and Ultraflex stents in the treatment of malignant stenoses of the esophagus and cardia.

    Science.gov (United States)

    Dorta, G; Binek, J; Blum, A L; Bühler, H; Felley, C P; Koelz, H R; Lammer, F; Lang, C; Meier, R; Meyenberger, C; Meyer-Wyss, B; Michetti, P; Protiva, P; Scheurer, U; Weber, B; Wiesel, P; Vogel, S

    1997-03-01

    Several published studies have examined various self-expanding metal esophageal stents for use in the palliative treatment of esophageal or cardiac neoplasia, but few have compared different self-expanding metal stents. The aim of this study was to evaluate non-covered Wallstent and Ultraflex prostheses in the treatment of malignancies in the esophagus and the cardiac region. In a retrospective study, the effectiveness of non-covered Wallstents (46 patients) and Ultraflex stents (36 patients) was compared in the treatment of malignancies in the esophageal and cardiac regions. Reintervention procedures were necessary in 16 of the 46 Wallstent patients (six patients during an early phase) and in 22 of the 36 Ultraflex patients (13 during an early phase) (overall P = 0.022; early P = 0.018). The major complication in the Wallstent group was tumor ingrowth (12 of 35 complications), while in the Ultraflex group, it was incomplete deployment (18 of 49 complications). Incomplete stent deployment occurred more often in patients treated with Ultraflex (P = 0.01), and food impaction was more often observed in the Wallstent group (P = 0.001). In addition, in patients with Ultraflex stents, more complex reinterventions were necessary than those required with Wallstents (four vs. 13 complex reinterventions, P = 0.0046). Wallstents tended to improve dysphagia better than Ultraflex stents. Compared to Ultraflex stents, Wallstents have several significant short-term and long-term advantages in the palliative treatment of malignancy of the esophagus and cardia.

  6. Neogene and active shortening offshore the reactivated Levant margin in Lebanon: results of the SHALIMAR cruise

    Science.gov (United States)

    Briais, A.; Singh, S. C.; Tapponnier, P.; Elias, A.; Sursock, A.; Jomaa, R.; Carton, H.; Daeron, M.; King, G.; Jacques, E.

    2004-12-01

    The objectives of the SHALIMAR cruise were to study recent deformation of the Mediterranean seafloor west of Mt Lebanon. We collected multibeam bathymetry and back-scatter images, reflection seismic profiles - surface and deep-towed, 3.5 kHz echo-sounder data, gravity and magnetic data over an 80 km-wide zone offshore the entire Lebanese coast. The bathymetry reveals a very steep slope between Beyrouth and Batroun, with a water depth of 1500 m only 5 km offshore. Between Saida and Tripoli (33.5N to 34.5N), both the bathymetry and seismic lines show a series of ramp anticlines affecting Plio-Quaternary deposits and a seismically transparent layer with variable thickness corresponding to the Messinian evaporites (5.6 Ma). This fold-and-thrust belt is the offshore expression of shortening related to the formation of Mt Lebanon. It is limited westwards by a prominent fold front 30 km from shore. Some ramps appear to be submarine continuations of faults documented ashore (e.g., Aabde and Tripoli thrusts). The strikes of fold axes are consistent with WNW-ESE shortening and slip-partitioning along the 30° Lebanese bend of the Levant fault. North of Tripoli and south of Saida, the continental margin displays a wider shelf (20 km) and gentler slope. In the south, at 1200-1500 m depth small, closely spaced, NE-trending scarps attest to young, distributed dip-slip faulting, although deformation is much less than north of Beyrouth. At the southern extremity of our survey, NW-trending normal fault scarps roughly aligned with the Mt Carmel-Haifa fault vanish 50 km offshore in the Levant basin. Bouguer gravity anomalies, estimated by removing from free-air gravity data the effect of bathymetry, display a very steep gradient between Beyrouth and Tripoli, marking the passage from thickened Mt Lebanon crust to thin crust in the Levant basin. Gravity anomalies also outline large, NE-SW trending steps between the basin crust and thinned continental crust near and south of Beyrouth

  7. The curve shortening problem

    CERN Document Server

    Chou, Kai-Seng

    2001-01-01

    Although research in curve shortening flow has been very active for nearly 20 years, the results of those efforts have remained scattered throughout the literature. For the first time, The Curve Shortening Problem collects and illuminates those results in a comprehensive, rigorous, and self-contained account of the fundamental results.The authors present a complete treatment of the Gage-Hamilton theorem, a clear, detailed exposition of Grayson''s convexity theorem, a systematic discussion of invariant solutions, applications to the existence of simple closed geodesics on a surface, and a new, almost convexity theorem for the generalized curve shortening problem.Many questions regarding curve shortening remain outstanding. With its careful exposition and complete guide to the literature, The Curve Shortening Problem provides not only an outstanding starting point for graduate students and new investigations, but a superb reference that presents intriguing new results for those already active in the field.

  8. Evaluation of EMS insertion and radiotherapy for malignant biliary stricture. Endoscopic bilateral hepatic drainage with wallstents

    Energy Technology Data Exchange (ETDEWEB)

    Kin, Hideyuki; Sumitomo, Yasuhiko; Kiriya, Keiichi [Shizuoka General Hospital (Japan)] [and others

    1998-10-01

    Usefulness of radiotherapy for unresectable malignant biliary obstruction was evaluated. Patients were 62 cases having inside stoma by expandable metallic stent (EMS) (male: 28 cases, female: 34 cases, mean age: 65.4-year-old (34-91)). In 6 cases of porta hepatis obstruction, bilateral hepatic drainage was tried using wallstent. Thirty-five patients received external irradiation with average of 39 (25-65) Gy, and the EMS was inserted during radiotherapy. Patency period after EMS insertion and survival time were compared with those of the group of EMS without irradiation. As for patency period of EMS, there was no significant difference between EMS group without irradiation and group with external irradiation. The 50% cumulative survival time was 132 days and 208 days, respectively. There was significant elongation of survival time in the group with external irradiation. (K.H.)

  9. The "Wallstent"

    DEFF Research Database (Denmark)

    Beier-Holgersen, R; Brasso, K; Nordling, J

    1993-01-01

    Ten patients have been reviewed in whom urethral stents were implanted for treatment of urethral stricture. Their urethral strictures had been treated with a median of 4.2 endoscopic urethrotomies under direct vision without success. The patients were first treated with optical urethrotomy, and d...

  10. Shortening filtrations

    Institute of Scientific and Technical Information of China (English)

    ENOCHS Edgar E.

    2012-01-01

    Let C be a set of modules.We argue that there is an ordinal κ such that if a module has a filtration by modules in C,then it has a filtration of length κ by direct sums of modules in C.As an application we give another way to prove a result of Saorín and (S)(t)oví(c)ek and of (S)(t)oví(c)ek.

  11. Hepatitis C RNA assay differences in results: Potential implications for shortened therapy and determination of Sustained Virologic Response

    Science.gov (United States)

    Cloherty, Gavin; Chevaliez, Stephane; Sarrazin, Christoph; Herman, Christine; Holzmayer, Vera; Dawson, George; Maasoumy, Benjamin; Vermehren, Johannes; Wedemeyer, Heiner; Feld, Jordan J.; Pawlotsky, Jean-Michel

    2016-01-01

    Approval of Ledipasvir/Sofosbuvir for the treatment of chronic hepatitis C (HCV) includes the truncation of therapy from 12 to 8 weeks in treatment naïve, non-cirrhotic patients with baseline HCV RNA levels <6 million IU/mL (6.8 log10 IU/mL). The aim of this study was to evaluate this clinical cutoff with a different widely used commercially available HCV RNA test. Results from samples tested prospectively with Roche High Pure TaqMan HCV 2.0 test (HPS) were compared to those tested retrospectively with the Abbott RealTime HCV RNA test (ART). Using 6 million IU/mL as the cut-off, pre-treatment results were concordant in 70.4% of cases. When results with the same test measured at screening and baseline, clinical decisions could be impacted in 14.4% and 6.2% of cases for HPS and ART respectively. Using only HCV RNA cutoff of 6 million IU/mL, 29.55% of subjects would receive a different and potentially incorrect treatment duration based solely on HCV RNA test method used. A further 6–14% of subjects would have treatment decision change based on the day the sample was taken. PMID:27762283

  12. Quantitative angiographic follow-up of the coronary wallstent in native vessels and bypass grafts (European experience - March 1986 to March 1990)

    NARCIS (Netherlands)

    B.H. Strauss (Bradley); P.W.J.C. Serruys (Patrick); M.E. Bertrand (Michel); J. Puel (Jacques); B. Meier (Bernard); J-J. Goy (Jean-Jacques); L. Kappenberger (Lukas); A.F. Rickards (Anthony); U. Sigwart (Ulrich); M-A.M. Morel (Marie-Angèle); E.W.J. Montauban van Swijndregt (Eline)

    1992-01-01

    textabstractThe coronary stent has been investigated as an adjunct to percutaneous transluminal coronary angioplasty to obviate the problems of early occlusion and late restenosis. From March 1986 to March 1990, 265 patients (308 lesions) were implanted with the coronary Wallstent in 6 European cent

  13. Shortening Scarf osteotomy for correction of severe hallux valgus. Does shortening affect the outcome?

    Science.gov (United States)

    Karpe, Prasad; Killen, Marie C; Pollock, Raymond D; Limaye, Rajiv

    2016-12-01

    Translation and shortening of Scarf osteotomy allows correction of severe hallux valgus deformity. Shortening may result in transfer metatarsalgia. To evaluate outcome of patients undergoing shortening Scarf osteotomy for severe hallux valgus deformities. Fifteen patients (20feet, mean age 58 years) underwent shortening Scarf osteotomy for severe hallux valgus deformities. Outcomes were pre and postoperative AOFAS scores, IM and HV angles, patient satisfaction. Mean follow-up was 25 months (range 22-30). The IM angle improved from a median of 18.60 (range 13.4-26.20) preoperatively to 9.70 (range 8.0-13.70) postoperatively (8.9; 95% CI=7.6-10.3; posteotomies united. Shortening Scarf osteotomy is a viable option for treating severe hallux valgus deformities with no transfer metatarsalgia. Copyright © 2016 Elsevier Ltd. All rights reserved.

  14. Occlusal stability in shortened dental arches.

    Science.gov (United States)

    Witter, D J; Creugers, N H; Kreulen, C M; de Haan, A F

    2001-02-01

    Shortened dental arches consisting of anterior and premolar teeth have been shown to meet oral functional demands. However, the occlusal stability may be at risk as a result of tooth migration. The aim of this nine-year study was to investigate occlusal stability in shortened dental arches as a function over time. Occlusal stability indicators were: 'interdental spacing', 'occlusal contacts of anterior teeth in Intercuspal Position', 'overbite', 'occlusal tooth wear', and 'alveolar bone support'. Subjects with shortened dental arches (n = 74) were compared with subjects with complete dental arches (controls, n = 72). Repeated-measurement regression analyses were applied to assess age-dependent variables in the controls and to relate the occlusal changes to the period of time since the treatment that led to the shortened dental arches. Compared with complete dental arches, shortened dental arches had similar overbite and occlusal tooth wear. They showed more interdental spacing in the premolar regions, more anterior teeth in occlusal contact, and lower alveolar bone scores. Since the differences remained constant over time, we conclude that shortened dental arches can provide long-term occlusal stability. Occlusal changes were self-limiting, indicating a new occlusal equilibrium.

  15. Shortening Anomalies in Supersymmetric Theories

    CERN Document Server

    Gomis, Jaume; Ooguri, Hirosi; Seiberg, Nathan; Wang, Yifan

    2016-01-01

    We present new anomalies in two-dimensional ${\\mathcal N} =(2, 2)$ superconformal theories. They obstruct the shortening conditions of chiral and twisted chiral multiplets at coincident points. This implies that marginal couplings cannot be promoted to background super-fields in short representations. Therefore, standard results that follow from ${\\mathcal N} =(2, 2)$ spurion analysis are invalidated. These anomalies appear only if supersymmetry is enhanced beyond ${\\mathcal N} =(2, 2)$. These anomalies explain why the conformal manifolds of the K3 and $T^4$ sigma models are not K\\"ahler and do not factorize into chiral and twisted chiral moduli spaces and why there are no ${\\mathcal N} =(2, 2)$ gauged linear sigma models that cover these conformal manifolds. We also present these results from the point of view of the Riemann curvature of conformal manifolds.

  16. Shortening induced effects on force (re)development in pig urinary smooth muscle

    OpenAIRE

    van Asselt, Els; Pel, Johan; van Mastrigt, Ron

    2007-01-01

    textabstractIntroduction: When muscle is allowed to shorten during an active contraction, the maximum force that redevelops after shortening is smaller than the isometric force at the same muscle length without prior shortening. We studied the course of force redevelopment after shortening in smooth muscle to unravel the mechanism responsible for this deactivation. Method: In a first series of measurements the shortening velocity was varied resulting in different shortening amplitudes. In a s...

  17. Shortening the Xerostomia Inventory

    Science.gov (United States)

    Thomson, William Murray; van der Putten, Gert-Jan; de Baat, Cees; Ikebe, Kazunori; Matsuda, Ken-ichi; Enoki, Kaori; Hopcraft, Matthew; Ling, Guo Y

    2011-01-01

    Objectives To determine the validity and properties of the Summated Xerostomia Inventory-Dutch Version in samples from Australia, The Netherlands, Japan and New Zealand. Study design Six cross-sectional samples of older people from The Netherlands (N = 50), Australia (N = 637 and N = 245), Japan (N = 401) and New Zealand (N = 167 and N = 86). Data were analysed using the Summated Xerostomia Inventory-Dutch Version. Results Almost all data-sets revealed a single extracted factor which explained about half of the variance, with Cronbach’s alpha values of at least 0.70. When mean scale scores were plotted against a “gold standard” xerostomia question, statistically significant gradients were observed, with the highest score seen in those who always had dry mouth, and the lowest in those who never had it. Conclusion The Summated Xerostomia Inventory-Dutch Version is valid for measuring xerostomia symptoms in clinical and epidemiological research. PMID:21684773

  18. Numerical Simulation of Pulse Shortening in RBWOs

    Institute of Scientific and Technical Information of China (English)

    GONG Yu-bin; ZHANG Zhang; WANG Wen-xiang; MENG Fan-bao; FAN Zhi-kai; HUANG Min-zhi

    2005-01-01

    Pulse shortening hinders improvement of microwave output energy for high power microwave tubes. So far, it is also an unresolved problem in the field of high power microwave devices. In this paper, relativistic backward wave tube (RBWO) is treated as an example to study the pulse shortening phenomena. The influences of gas existing in the tube and explosive emission in inner surface of RBWO are all investigated by means of the particle-in-cell method. Through the simulation results, it can be predicted that the background gas in the tube is one but not the most important factor resulting in pulse shortening, in order to broaden the pulse width of gas-filled RBWO, the pressure of the filled gas must be controlled in a proper value. The explosive emission in the surface of slow wave structure due to intense electric field is one of the most important factors causing pulse shortening in high power microwave tube.Some methods to overcome this find of explosive emission are also given.

  19. Shortening amplitude affects the incomplete force recovery after active shortening in mouse soleus muscle.

    Science.gov (United States)

    Van Noten, Pieter; Van Leemputte, Marc

    2009-12-11

    Compared to isometric contraction, the force producing capacity of muscle is reduced (force depression, FD) after a work producing shortening phase. It has been suggested that FD results from an inhibition of cross-bridge binding. Because the rate constants of the exponential force (re)development are thought to be primarily determined by cross-bridge attachment/detachment rate, we aimed to investigate the components of force redevelopment (REDEV) after 0.6, 1.2 and 2.4mm shortening, resulting in varying amounts of FD (from about 5% to about 16%), in mouse soleus muscle (n=11). Compared to isometric force development (DEV), the time to reach steady-state during REDEV was about 3 times longer (370 versus 1261ms) increasing with increasing amplitude. Contrary to a single, a double exponential function with one component set equal to the rate constant of DEV (14.3s(-1)), accurately described REDEV (RMSshortening amplitude and was associated with work delivered during shortening (R(2)=0.75) and FD (R(2)=0.77). We concluded that a work related slow exponential component is induced to the trajectory of incomplete force recovery after shortening, causing FD. These results suggest that after shortening, aside from cross-bridges with normal attachment/detachment rate, cross-bridges with reduced cycling rate are active.

  20. Shortening of subjective visual intervals followed by repetitive stimulation.

    Directory of Open Access Journals (Sweden)

    Fuminori Ono

    Full Text Available Our previous research demonstrated that repetitive tone stimulation shortened the perceived duration of the preceding auditory time interval. In this study, we examined whether repetitive visual stimulation influences the perception of preceding visual time intervals. Results showed that a time interval followed by a high-frequency visual flicker was perceived as shorter than that followed by a low-frequency visual flicker. The perceived duration decreased as the frequency of the visual flicker increased. The visual flicker presented in one hemifield shortened the apparent time interval in the other hemifield. A final experiment showed that repetitive tone stimulation also shortened the perceived duration of preceding visual time intervals. We concluded that visual flicker shortened the perceived duration of preceding visual time intervals in the same way as repetitive auditory stimulation shortened the subjective duration of preceding tones.

  1. The effect of shortening history on isometric and dynamic muscle function.

    Science.gov (United States)

    McDaniel, John; Elmer, Steven J; Martin, James C

    2010-03-03

    Despite numerous reports on isometric force depression, few reports have quantified force depression during active muscle shortening (dynamic force depression). The purpose of this investigation was to determine the influence of shortening history on isometric force following active shortening, force during isokinetic shortening, and velocity during isotonic shortening. The soleus muscles of four cats were subjected to a series of isokinetic contractions at three shortening velocities and isotonic contractions under three loads. Muscle excursions initiated from three different muscle lengths but terminated at a constant length. Isometric force produced subsequent to active shortening, and force or shortening velocity produced at a specific muscle length during shortening, were compared across all three conditions. Results indicated that shortening history altered isometric force by up to 5%, force during isokinetic shortening up to 30% and shortening velocity during isotonic contractions by up to 63%. Furthermore, there was a load by excursion interaction during isotonic contractions such that excursion had the most influence on shortening velocity when the loads were the greatest. There was not a velocity by excursion interaction during isokinetic contractions. Isokinetic and isotonic power-velocity relationships displayed a downward shift in power as excursions increased. Thus, to discuss force depression based on differences in isometric force subsequent to active shortening may underestimate its importance during dynamic contractions. The presence of dynamic force depression should be realized in sport performance, motor control modeling and when controlling paralyzed limbs through artificial stimulation.

  2. T2 shortening in childhood moyamoya disease

    Energy Technology Data Exchange (ETDEWEB)

    Takanashi, J. [Department of Pediatrics, Faculty of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba-shi 260 (Japan); Sugita, K. [Department of Pediatrics, Faculty of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba-shi 260 (Japan); Tanabe, Y. [Division of Neurology, Chiba Children`s Hospital, 579-1 Heta, Midori-ku, Chiba-shi 266 (Japan); Ito, C. [Division of Neurosurgery, Chiba Children`s Hospital, 579-1 Heta, Midori-ku, Chiba-shi 266 (Japan); Date, H. [Division of Neurosurgery, Chiba Children`s Hospital, 579-1 Heta, Midori-ku, Chiba-shi 266 (Japan); Niimi, H. [Department of Pediatrics, Faculty of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba-shi 260 (Japan)

    1996-05-01

    We examined T2 shortening in six children with infarcts due to moyamoya disease to clarify whether there are characteristic patterns of T2 shortening in the deep grey and white matter. Profound T2 shortening in the deep grey and white matter was observed in the acute stage of infarct in two cases, which changed to high intensity in the chronic stage; in this stage no T2 shortening was demonstrated in any case. Neither haemorrhagic infarction nor calcification was seen on CT or MRI. There could be longitudinally different T2 shortening patterns between infarcts due to moyamoya disease and other disorders. (orig.). With 2 figs., 1 tab.

  3. Phenomenological models of the dynamics of muscle during isotonic shortening.

    Science.gov (United States)

    Yeo, Sang Hoon; Monroy, Jenna A; Lappin, A Kristopher; Nishikawa, Kiisa C; Pai, Dinesh K

    2013-09-27

    We investigated the effectiveness of simple, Hill-type, phenomenological models of the force-length-velocity relationship for simulating measured length trajectories during muscle shortening, and, if so, what forms of the model are most useful. Using isotonic shortening data from mouse soleus and toad depressor mandibulae muscles, we showed that Hill-type models can indeed simulate the shortening trajectories with sufficiently good accuracy. However, we found that the standard form of the Hill-type muscle model, called the force-scaling model, is not a satisfactory choice. Instead, the results support the use of less frequently used models, the f-max scaling model and force-scaling with parallel spring, to simulate the shortening dynamics of muscle.

  4. [Shortening arthrodesis of three wrist bones].

    Science.gov (United States)

    Delattre, O; Dupont, P; Reau, A F; Rouvillain, J L; Mousselard, H; Catonné, Y

    1997-01-01

    In advanced cases of wrist osteoarthritis with lesions of the radio-scaphoid and mediocarpal joints, and when a proximal row carpectomy is not possible because of lesions of the head of the capitate, we suggest a new technique: The hamate-capitate-lunate shortening arthrodesis with a scaphoid-triquetral resection. The good results observed with proximal row carpectomies, and particularly their long-term reliability, have encouraged us on this new path. Effectively, this operation takes the concept of proximal row carpectomy one step further by reconstructing the head of the capitate with the lunate whose proximal articular surface is often not deteriorated even in very advanced cases of radio and mediocarpal osteoarthritis. The two theoretical concepts of this operation are the shortening of the carpus and respect of the physiological congruence of the radio-lunate joint, the goal being obtain similar results those with proximal row carpectomy, particularly concerning mobility. We present our first two cases with this technique. This new procedure is an alternative to the four bone arthrodesis, particularly in SLAC wrist sequellae when they have evolved to the stage of radio and mediocarpal osteoarthritis.

  5. Augmentation of deglutitive thyrohyoid muscle shortening by the Shaker Exercise.

    Science.gov (United States)

    Mepani, Rachel; Antonik, Stephen; Massey, Benson; Kern, Mark; Logemann, Jerilyn; Pauloski, Barbara; Rademaker, Alfred; Easterling, Caryn; Shaker, Reza

    2009-03-01

    Earlier studies of the effect of 6 weeks of the Shaker Exercise have shown significant increase in UES opening and anterior excursion of larynx and hyoid during swallowing in patients with upper esophageal sphincter (UES) dysfunction, resulting in elimination of aspiration and resumption of oral intake. This effect is attributed to strengthening of the suprahyoid muscles, as evidenced by comparison of electromyographic changes in muscle fatigue before and after completion of the exercise regime. The effect of this exercise on thyrohyoid muscle shortening is unknown. Therefore the aim of this study was to determine the effect of the exercise on thyrohyoid muscle shortening. We studied 11 dysphagic patients with UES dysfunction. Six were randomized to traditional swallowing therapy and five to the Shaker Exercise. Videofluoroscopy was used to measure deglutitive thyrohyoid shortening before and after completion of assigned therapy regimen. Maximum thyrohyoid muscle shortening occurred at close temporal proximity to the time of maximal thyroid cartilage excursion. The percent change in thyrohyoid distance from initiation of deglutition to maximal anterior/superior hyoid excursion showed no statistically significant difference between the two groups prior to either therapy (p = 0.54). In contrast, after completion of therapy, the percent change in thyrohyoid distance in the Shaker Exercise group was significantly greater compared to the traditional therapy (p = 0.034). The Shaker Exercise augments the thyrohyoid muscle shortening in addition to strengthening the suprahyoid muscles. The combination of increased thyrohyoid shortening and suprahyoid strengthening contributes to the Shaker Exercise outcome of deglutitive UES opening augmentation.

  6. Biochemical response to chronic shortening in unloaded soleus muscles

    Science.gov (United States)

    Jaspers, S. R.; Fagan, J. M.; Tischler, M. E.

    1985-01-01

    One leg of tail-casted suspended rats was immobilized in a plantar-flexed position to test whether chronic shortening of posterior leg muscles affected the metabolic response to unloading. The immobilized plantaris and gastrocnemius muscles of these animals showed approximately 20 percent loss of muscle mass in contrast to simply a slower growth rate with unloading. Loss of mass of the soleus muscle during suspension was not accentuated by chronic shortening. Although protein degradation in the isolated soleus muscle of the plantar-flexed limb was slightly faster than in the contralateral free limb, this difference was offset by faster synthesis of the myofibrillar protein fraction of the chronically shortened muscle. Total adenine nucleotides were 17 percent lower (P less than 0.005) in the chronically shortened soleus muscle following incubation. Glutamate, glutamine, and alanine metabolism showed little response to chronic shortening. These results suggest that, in the soleus muscle, chronic shortening did not alter significantly the metabolic responses to unloading and reduced activity.

  7. Consequences of crown shortening canine teeth in Greenland sled dogs

    DEFF Research Database (Denmark)

    Kortegaard, H E; Anthony Knudsen, T; Dahl, S

    2015-01-01

    OBJECTIVES: To evaluate the consequences of crown shortening, focusing on the prevalence of pulp exposure and periapical pathology in Greenland sled dogs that had had their canine crowns shortened at an early age. METHODS: Five cadaver heads and 54 sled dogs underwent an oral examination for dental...... fractures and pulp exposure of canines. All canines were radiographed and evaluated for periapical pathology. RESULTS: The prevalence of canine pulp exposure in 12 (5 heads and 7 dogs) crown shortened dogs was 91 · 7%, and 21 · 3% in 47 not-crown shortened dogs. A significant (P ... exposure of the canines in the crown shortened group compared to the not-crown shortened group was seen with a relative risk of 4 · 3 on a dog basis and a relative risk of 12 · 2 on a tooth basis. In dogs with pulp exposure of canines (n = 51) the prevalence of periapical pathology was 82 · 4%, but only 0...

  8. 10 CFR 590.316 - Shortened proceedings.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Shortened proceedings. 590.316 Section 590.316 Energy... WITH RESPECT TO THE IMPORT AND EXPORT OF NATURAL GAS Procedures § 590.316 Shortened proceedings. In any proceeding where, in response to a notice of application or notice of procedures, if applicable, no...

  9. The mechanics of mouse skeletal muscle when shortening during relaxation.

    Science.gov (United States)

    Barclay, C J; Lichtwark, G A

    2007-01-01

    The dynamic properties of relaxing skeletal muscle have not been well characterised but are important for understanding muscle function during terrestrial locomotion, during which a considerable fraction of muscle work output can be produced during relaxation. The purpose of this study was to characterise the force-velocity properties of mouse skeletal muscle during relaxation. Experiments were performed in vitro (21 degrees C) using bundles of fibres from mouse soleus and EDL muscles. Isovelocity shortening was applied to muscles during relaxation following short tetanic contractions. Using data from different contractions with different shortening velocities, curves relating force output to shortening velocity were constructed at intervals during relaxation. The velocity component included contributions from shortening of both series elastic component (SEC) and contractile component (CC) because force output was not constant. Early in relaxation force-velocity relationships were linear but became progressively more curved as relaxation progressed. Force-velocity curves late in relaxation had the same curvature as those for the CC in fully activated muscles but V(max) was reduced to approximately 50% of the value in fully activated muscles. These results were the same for slow- and fast-twitch muscles and for relaxation following maximal tetani and brief, sub-maximal tetani. The measured series elastic compliance was used to partition shortening velocity between SEC and CC. The curvature of the CC force-velocity relationship was constant during relaxation. The SEC accounted for most of the shortening and work output during relaxation and its power output during relaxation exceeded the maximum CC power output. It is proposed that unloading the CC, without any change in its overall length, accelerated cross-bridge detachment when shortening was applied during relaxation.

  10. Trans Fatty Acid content in Danish margarines and shortenings

    DEFF Research Database (Denmark)

    Leth, Torben; Bysted, Anette; Hansen, Kirsten

    2003-01-01

    in shortenings, averaging about 6-7%. Long-chain TFA from hydrogenated,fish oil, although present in 13 brands in 1995, were not found at all in the 1999 samples. Trans-linoleic acids or CLA were not found. The reduction in TFA content in margarines has not resulted in a systematic change over the years......Margarines and shortenings have been major contributors to the intake by humans of the probably atherogenic trans FA (TFA). In 1999, all 73 brands of margarines and shortenings on the Danish market were analyzed by GLC on a 50-m highly polar capillary column, and the results were compared...... in the content of saturated FA, monounsaturated FA, or PUFA. Calculated from sales figures, the intake of TFA decreased from 2.2 g per capita per year in 1992, to 1.5 g in 1995, and to 0.4 g in, 1999....

  11. Frying performance of palm-based solid frying shortening.

    Science.gov (United States)

    Omar, M N; Nor-Nazuha, M N; Nor-Dalilah, M N; Sahri, M M

    2010-03-15

    In order to evaluate the frying performance of palm-based solid frying shortening against standard olein, the fresh potato chips were fried in both frying media using an open fryer. After frying the chips for 40 h in an open batch fryer, it was found that the frying quality of palm-based solid frying shortening was better than standard palm olein in terms of Free Fatty Acid (FFA) values, Total Polar Content (TPC) and Total Polymeric Material (TPM). Solid shortening gave FFA, TPC and TPM values of 0.7, 15.3 and 2.67%, respectively, whilst standard palm olein gave values for FFA, TPC and TPM of 1.2, 19.6 and 3.10%, respectively. In terms of sensory mean scores, sensory panelists preferred the color of potato chips fried in solid shortening on the first day of frying, while on the third and fifth day of frying there were no significant differences (p palm olein when used for deep fat frying in terms of FFA values, total polar content and total polymeric material, especially for starch-based products such as potato chips. The result also shows that, in terms of sensory mean scores, after frying for 40 h, the sensory panelists gave higher scores in terms of taste, flavor and crispiness for potato chips fried in palm-based solid shortening.

  12. Child Abuse May Shorten Some Women's Lives

    Science.gov (United States)

    ... page: https://medlineplus.gov/news/fullstory_160478.html Child Abuse May Shorten Some Women's Lives Extreme stress may ... 300 middle-aged U.S. adults, female survivors of child abuse were more likely to die over the next ...

  13. Can Your Heartburn Meds Shorten Your Life?

    Science.gov (United States)

    ... gov/news/fullstory_167005.html Can Your Heartburn Meds Shorten Your Life? Study found association between prolonged ... of Medicine, in St. Louis. "There was a relationship between duration of use and risk of death," ...

  14. Accelerated Telomere Shortening in Acromegaly; IGF-I Induces Telomere Shortening and Cellular Senescence

    Science.gov (United States)

    Matsumoto, Ryusaku; Fukuoka, Hidenori; Iguchi, Genzo; Odake, Yukiko; Yoshida, Kenichi; Bando, Hironori; Suda, Kentaro; Nishizawa, Hitoshi; Takahashi, Michiko; Yamada, Shozo; Ogawa, Wataru; Takahashi, Yutaka

    2015-01-01

    Objective Patients with acromegaly exhibit reduced life expectancy and increased prevalence of age-related diseases, such as diabetes, hypertension, and cardiovascular disease. However, the underlying mechanism has not been fully elucidated. Telomere shortening is reportedly associated with reduced life expectancy and increased prevalence of these age-related diseases. Methods We measured telomere length in patients with acromegaly using quantitative PCR method. The effect of GH and IGF-I on telomere length and cellular senescence was examined in human skin fibroblasts. Results Patients with acromegaly exhibited shorter telomere length than age-, sex-, smoking-, and diabetes-matched control patients with non-functioning pituitary adenoma (0.62 ± 0.23 vs. 0.75 ± 0.35, respectively, P = 0.047). In addition, telomere length in acromegaly was negatively correlated with the disease duration (R2 = 0.210, P = 0.003). In vitro analysis revealed that not GH but IGF-I induced telomere shortening in human skin fibroblasts. Furthermore, IGF-I-treated cells showed increased senescence-associated β-galactosidase activity and expression of p53 and p21 protein. IGF-I-treated cells reached the Hayflick limit earlier than GH- or vehicle-treated cells, indicating that IGF-I induces cellular senescence. Conclusion Shortened telomeres in acromegaly and cellular senescence induced by IGF-I can explain, in part, the underlying mechanisms by which acromegaly exhibits an increased morbidity and mortality in association with the excess secretion of IGF-I. PMID:26448623

  15. Does the speed of shortening affect steady-state force depression in cat soleus muscle?

    Science.gov (United States)

    Leonard, T R; Herzog, W

    2005-11-01

    It has been stated repeatedly for the past 50 years that the steady-state force depression following shortening of an activated muscle depends on the speed of shortening. However, these statements were based on results from experiments in which muscles were shortened at different speeds but identical activation levels. Therefore, the force during shortening was changed in accordance with the force-velocity relationship of muscles: that is, increasing speeds of shortening were associated with decreasing forces, and vice versa. Consequently, it is not possible at present to distinguish whether force depression is caused by the changes in speed, as frequently stated, or the associated changes in force, or both. The purpose of this study was to test if force depression depends on the speed of shortening. We hypothesized that force depression was dependent on the force but not the speed of contraction. Our prediction is that the amount of force depression after shortening contractions at different speeds could be similar if the force during contraction was controlled at a similar level. Cat soleus muscles (n=7) were shortened by 9 or 12 mm at speeds of 3, 9, and 27 mm/s, first with a constant activation during shortening (30Hz), then with activation levels that were reduced (shortening forces of the fast speed contractions (27 mm/s). If done properly, force depression could be precisely matched at the three different speeds, indicating that force depression was related to the force during the shortening contraction but not to the speed. However, in order to match force depression, the forces during shortening had to be systematically greater for the slow compared to the fast speeds of shortening, suggesting that force depression also depends on the level of activation, as force depression at constant activation levels can only be matched if the force during shortening, evaluated by the mechanical work, is identical. Therefore, we conclude that force depression depends

  16. Residual force enhancement following shortening is speed-dependent

    Science.gov (United States)

    Fortuna, Rafael; Power, Geoffrey A.; Mende, Esther; Seiberl, Wolfgang; Herzog, Walter

    2016-01-01

    The steady-state isometric force following active muscle shortening or lengthening is smaller (force depression; FD) or greater (residual force enhancement; RFE) than a purely isometric contraction at the corresponding length. The mechanisms underlying these phenomena are not explained within the context of the cross-bridge theory and are rarely studied in concert. Previous studies have shown RFE to be speed-independent. In the present study, we investigated if RFE preceded by active shortening is time-dependent by electrically evoking RFE in the human adductor pollicis muscle. The results shown that a slow stretch following FD fully re-established RFE compared to higher speeds of stretch. The mechanism(s) responsible for the recovery of RFE following a preceding shortening contraction (FD) might be associated with the recovery of cross-bridge based force and/or the re-engagement of a passive structural element (titin). Voluntary interaction with one’s environment involves highly coordinated shortening and lengthening muscle contractions. Therefore comprehending these history-dependent muscle properties in the context of movement control is paramount in understanding the behavior of in vivo motor control. PMID:26869508

  17. Telomere shortening may be associated with human keloids

    Directory of Open Access Journals (Sweden)

    Wilson Robert R

    2009-10-01

    Full Text Available Abstract Background Keloids are benign skin tumors that are the effect of a dysregulated wound-healing process in genetically predisposed patients. They are inherited with an autosomal dominant mode with incomplete clinical penetrance and variable expression. Keloids are characterized by formation of excess scar tissue beyond the boundaries of the wound. The exact etiology is still unknown and there is currently no appropriate treatment for keloid disease. Methods We analyzed sample tissues were obtained from 20 patients with keloid skin lesions and normal skin was obtained from 20 healthy donors. The telomeres were measured by Terminal Restriction Fragment (TRF analysis and Real-Time PCR assay. Quantitative Real-Time RT-PCR analysis of hTERT gene expression was performed and intracellular ROS generation was measured. Results In this study, we determined whether telomeric shortening and the expression of human telomerase reverse transcriptase (hTERT occurs in keloid patients. Using Terminal Restriction Fragment (TRF analysis and Real-Time PCR assay, we detected a significant telomere shortening of 30% in keloid specimens compared to normal skin. Using quantitative Real-Time RT-PCR, telomerase activity was found absent in the keloid tissues. Moreover, an increase in ROS generation was detected in fibroblasts cell cultures from keloid specimens as more time elapsed compared to fibroblasts from normal skin. Conclusion Telomere shortening has been reported in several metabolic and cardiovascular diseases. We found that telomere shortening can also be associated with human keloids. Chronic oxidative stress plays a major role in the pathophysiology of several chronic inflammatory diseases. Here we found increased ROS generation in fibroblasts from keloid fibroblasts cell cultures when compared to normal skin fibroblasts. Hence we conclude that oxidative stress might be an important modulator of telomere loss in keloid because of the absence of active

  18. 9 CFR 319.701 - Mixed fat shortening.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Mixed fat shortening. 319.701 Section... INSPECTION AND CERTIFICATION DEFINITIONS AND STANDARDS OF IDENTITY OR COMPOSITION Fats, Oils, Shortenings § 319.701 Mixed fat shortening. Shortening prepared with a mixture of meat fats and vegetable oils...

  19. Shortened preoperative fasting for prevention of complications associated with laparoscopic cholecystectomy: a meta-analysis.

    Science.gov (United States)

    Xu, Duo; Zhu, Xuejiao; Xu, Yuan; Zhang, Liqing

    2017-02-01

    Objective Routine fasting (12 h) is always applied before laparoscopic cholecystectomy, but prolonged preoperative fasting causes thirst, hunger, and irritability as well as dehydration, low blood glucose, insulin resistance and other adverse reactions. We assessed the safety and efficacy of a shortened preoperative fasting period in patients undergoing laparoscopic cholecystectomy. Methods We searched PubMed, Embase and Cochrane Central Register of Controlled Trials up to 20 November 2015 and selected controlled trials with a shortened fasting time before laparoscopic cholecystectomy. We assessed the results by performing a meta-analysis using a variety of outcome measures and investigated the heterogeneity by subgroup analysis. Results Eleven trials were included. Forest plots showed that a shortened fasting time reduced the operative risk and patient discomfort. A shortened fasting time also reduced postoperative nausea and vomiting as well as operative vomiting. With respect to glucose metabolism, a shortened fasting time significantly reduced abnormalities in the ratio of insulin sensitivity. The C-reactive protein concentration was also reduced by a shortened fasting time. Conclusions A shortened preoperative fasting time increases patients' postoperative comfort, improves insulin resistance, and reduces stress responses. This evidence supports the clinical application of a shortened fasting time before laparoscopic cholecystectomy.

  20. Effects of stretch and shortening on gene expression in intact myocardium.

    Science.gov (United States)

    Haggart, Charles R; Ames, Elizabeth G; Lee, Jae K; Holmes, Jeffrey W

    2014-01-15

    Multiple cues have been suggested as the mechanical stimulus for the heart's hypertrophic response. Our work has previously suggested that the amount of cyclic shortening in cardiomyocytes controls myocyte shape and the amount of stretch controls myocyte size. To identify gene expression changes that occur in response to these mechanical perturbations, we used microarray analysis of papillary muscles cultured for 12 h at physiological or reduced levels of cyclic shortening and physiological or reduced mean stretch. Overall, genes related to extracellular matrix (ECM) were surprisingly prominent in our analysis. Connective tissue growth factor was among a small group of genes regulated by the amount of cyclic shortening regardless of the level of mean stretch, and many more ECM genes were regulated by shortening with reduced amounts of stretch. When we compared our results to gene expression data from an in vivo model of pressure overload (PO), which also decreases myocyte shortening, we found the genes that were commonly regulated in PO and our decreased shortening groups were most significantly enriched for ontology terms related to the ECM, followed by genes associated with mechanosensing and the cytoskeleton. The list of genes regulated in PO and our decreased shortening groups also includes genes known to change early in hypertrophy, such as myosin heavy chain 7, brain natriuretic peptide, and myosin binding protein C. We conclude that in intact myocardium, the amount of cyclic shortening may be an important regulator not only of myocyte genes classically associated with hypertrophy but also of ECM genes.

  1. Accumulating evidence for increased velocity of airway smooth muscle shortening in asthmatic airway hyperresponsiveness.

    Science.gov (United States)

    Ijpma, Gijs; Matusovsky, Oleg; Lauzon, Anne-Marie

    2012-01-01

    It remains unclear whether airway smooth muscle (ASM) mechanics is altered in asthma. While efforts have originally focussed on contractile force, some evidence points to an increased velocity of shortening. A greater rate of airway renarrowing after a deep inspiration has been reported in asthmatics compared to controls, which could result from a shortening velocity increase. In addition, we have recently shown in rats that increased shortening velocity correlates with increased muscle shortening, without increasing muscle force. Nonetheless, establishing whether or not asthmatic ASM shortens faster than that of normal subjects remains problematic. Endobronchial biopsies provide excellent tissue samples because the patients are well characterized, but the size of the samples allows only cell level experiments. Whole human lungs from transplant programs suffer primarily from poor patient characterization, leading to high variability. ASM from several animal models of asthma has shown increased shortening velocity, but it is unclear whether this is representative of human asthma. Several candidates have been suggested as responsible for increased shortening velocity in asthma, such as alterations in contractile protein expression or changes in the contractile apparatus structure. There is no doubt that more remains to be learned about the role of shortening velocity in asthma.

  2. Meat toughening does not occur when rigor shortening is prevented.

    Science.gov (United States)

    Koohmaraie, M; Doumit, M E; Wheeler, T L

    1996-12-01

    The objective of this experiment was to test the hypothesis that meat toughening during the first 24 h postmortem results from sarcomere shortening during rigor mortis development. Eleven market-weight lambs were used to measure changes in shear force of clamped longissimus during rigor development. Within 15 min of exsanguination, while attached at both ends, each longissimus was separated from the vertebrae body and clamped between three sets of metal plates to prevent muscle shortening (six clamped sections per lamb). Five of the clamped sections were placed at -1.1 degrees C for 0, 3, 6, 12, or 24 h. After storage at their respective times at -1.1 degrees C, the samples were placed at -30 degrees C for 90 min and then at -5 degrees C for 8 d. The sixth section (168-h section) was stored at -1.1 degrees C for the first 24 h, at 4 degrees C for 144 h, and then treated the same as other sampling times. Sections were sampled for pH, sarcomere length, shear force, and Western blot analyses before and after storage at -5 degrees C. Shear force values were the same (P > .05) from 0 to 24 h (4.5 kg at 0 h to 4.9 kg at 24 h) then declined (P slaughter is most likely due to sarcomere shortening.

  3. Accommodation of collisional shortening along the Alpine plate boundary : plate kinematics vs rheological controls

    Science.gov (United States)

    Rosenberg, Claudio; Bellahsen, Nicolas

    2016-04-01

    The style of collision in the Alps varies along strike, reflecting different amounts and different modes of accommodation of collisional shortening. These differences control the patterns of exhumation during collision. Whereas the western Alps largely consist of a metamorphic complex formed during subduction and largely exhumed before the initiation of collision, the subduction nappe-stack of the Central and the Eastern Alps is strongly overprinted by collisional shortening and by Barrovian metamorphism. Based on compiled and new data we estimate amounts of collisional shortening along the strike of the chain and set it in relationship to the geometry of the collisional prism. The western Alpine collisional structures form a very large (in map view), but moderately shortened wedge, terminating in front of a poorly developed Molasse basin. Shortening of this wedge was mainly localized along its external parts, resulting in accretion of basement and cover units thrusted towards the foreland. Back-folding and back-thrusting are barely developed and no shortening takes place in the upper, Adriatic plate. In the Central Alps, the amount of collisional shortening is larger and it is distributed both in the lower and in the upper plate. The collisional prism is bivergent and partitioning of the amount of shortening between the upper and lower plates varies along strike, being most probably controlled by rheological, heterogeneities. The thickened accreted lower plate is strongly affected by Barrovian metamorphism where shortening is largest and localized within a confined area. A deep Molasse basin developed in front of the prism. In the Eastern Alps collisional kinematics vary from east to west, with orogen-parallel displacements dominating in the east and orogen-perpendicular ones in the West, where they culminate in the structural and metamorphic dome of the Tauern Window. Nowhere else in the Alps collisional shortening is so strongly localized in one and the same

  4. Renal failure induces telomere shortening in the rat heart

    NARCIS (Netherlands)

    Wong, L. S.; Windt, W. A.; Roks, A. J.; van Dokkum, R. P.; Schoemaker, R. G.; de Zeeuw, D.; Henning, R. H.

    2009-01-01

    Background. Renal failure aggravates pathological cardiac remodelling induced by myocardial infarction (MI). Cardiac remodelling is associated with telomere shortening, a marker for biological ageing. We investigated whether mild and severe renal failure shorten cardiac telomeres and excessively sho

  5. Effects of muscle fibre shortening on the characteristics of surface motor unit potentials.

    Science.gov (United States)

    Rodriguez-Falces, Javier; Place, Nicolas

    2014-02-01

    Traditionally, studies dealing with muscle shortening have concentrated on assessing its impact on conduction velocity, and to this end, electrodes have been located between the end-plate and tendon regions. Possible morphologic changes in surface motor unit potentials (MUPs) as a result of muscle shortening have not, as yet, been evaluated or characterized. Using a convolutional MUP model, we investigated the effects of muscle shortening on the shape, amplitude, and duration characteristics of MUPs for different electrode positions relative to the fibre-tendon junction and for different depths of the MU in the muscle (MU-to-electrode distance). It was found that the effects of muscle shortening on MUP morphology depended not only on whether the electrodes were between the end-plate and the tendon junction or beyond the tendon junction, but also on the specific distance to this junction. When the electrodes lie between the end-plate and tendon junction, it was found that (1) the muscle shortening effect is not important for superficial MUs, (2) the sensitivity of MUP amplitude to muscle shortening increases with MU-to-electrode distance, and (3) the amplitude of the MUP negative phase is not affected by muscle shortening. This study provides a basis for the interpretation of the changes in MUP characteristics in experiments where both physiological and geometrical aspects of the muscle are varied.

  6. Multiple causes of fatigue during shortening contractions in rat slow twitch skeletal muscle.

    Science.gov (United States)

    Hortemo, Kristin Halvorsen; Munkvik, Morten; Lunde, Per Kristian; Sejersted, Ole M

    2013-01-01

    Fatigue in muscles that shorten might have other causes than fatigue during isometric contractions, since both cross-bridge cycling and energy demand are different in the two exercise modes. While isometric contractions are extensively studied, the causes of fatigue in shortening contractions are poorly mapped. Here, we investigate fatigue mechanisms during shortening contractions in slow twitch skeletal muscle in near physiological conditions. Fatigue was induced in rat soleus muscles with maintained blood supply by in situ shortening contractions at 37°C. Muscles were stimulated repeatedly (1 s on/off at 30 Hz) for 15 min against a constant load, allowing the muscle to shorten and perform work. Fatigue and subsequent recovery was examined at 20 s, 100 s and 15 min exercise. The effects of prior exercise were investigated in a second exercise bout. Fatigue developed in three distinct phases. During the first 20 s the regulatory protein Myosin Light Chain-2 (slow isoform, MLC-2s) was rapidly dephosphorylated in parallel with reduced rate of force development and reduced shortening. In the second phase there was degradation of high-energy phosphates and accumulation of lactate, and these changes were related to slowing of muscle relengthening and relaxation, culminating at 100 s exercise. Slowing of relaxation was also associated with increased leak of calcium from the SR. During the third phase of exercise there was restoration of high-energy phosphates and elimination of lactate, and the slowing of relaxation disappeared, whereas dephosphorylation of MLC-2s and reduced shortening prevailed. Prior exercise improved relaxation parameters in a subsequent exercise bout, and we propose that this effect is a result of less accumulation of lactate due to more rapid onset of oxidative metabolism. The correlation between dephosphorylation of MLC-2s and reduced shortening was confirmed in various experimental settings, and we suggest MLC-2s as an important regulator of

  7. Multiple causes of fatigue during shortening contractions in rat slow twitch skeletal muscle.

    Directory of Open Access Journals (Sweden)

    Kristin Halvorsen Hortemo

    Full Text Available Fatigue in muscles that shorten might have other causes than fatigue during isometric contractions, since both cross-bridge cycling and energy demand are different in the two exercise modes. While isometric contractions are extensively studied, the causes of fatigue in shortening contractions are poorly mapped. Here, we investigate fatigue mechanisms during shortening contractions in slow twitch skeletal muscle in near physiological conditions. Fatigue was induced in rat soleus muscles with maintained blood supply by in situ shortening contractions at 37°C. Muscles were stimulated repeatedly (1 s on/off at 30 Hz for 15 min against a constant load, allowing the muscle to shorten and perform work. Fatigue and subsequent recovery was examined at 20 s, 100 s and 15 min exercise. The effects of prior exercise were investigated in a second exercise bout. Fatigue developed in three distinct phases. During the first 20 s the regulatory protein Myosin Light Chain-2 (slow isoform, MLC-2s was rapidly dephosphorylated in parallel with reduced rate of force development and reduced shortening. In the second phase there was degradation of high-energy phosphates and accumulation of lactate, and these changes were related to slowing of muscle relengthening and relaxation, culminating at 100 s exercise. Slowing of relaxation was also associated with increased leak of calcium from the SR. During the third phase of exercise there was restoration of high-energy phosphates and elimination of lactate, and the slowing of relaxation disappeared, whereas dephosphorylation of MLC-2s and reduced shortening prevailed. Prior exercise improved relaxation parameters in a subsequent exercise bout, and we propose that this effect is a result of less accumulation of lactate due to more rapid onset of oxidative metabolism. The correlation between dephosphorylation of MLC-2s and reduced shortening was confirmed in various experimental settings, and we suggest MLC-2s as an

  8. Guidelines for reconstructing the metatarsal parabola with the shortening osteotomy.

    Science.gov (United States)

    Valley, B A; Reese, H W

    1991-08-01

    Lesser metatarsal pathology is difficult to treat surgically. The shortening osteotomy has shown promise as a useful technique. The following objectives are addressed in this study: 1) to discuss present techniques and two new radiographic measurement systems; 2) to establish mean, standard deviation, and normal range values for these measurement systems; 3) to determine if these measurements vary with foot size; and 4) to use the results to establish general guidelines for metatarsal parabola reconstruction. Means and normal ranges for the various radiographic measurements are identified to help the surgeon with preoperative evaluation and planning. The measurements vary mildly with foot size.

  9. Ulnar shortening after TFCC suture repair of Palmer type 1B lesions.

    Science.gov (United States)

    Wolf, Maya B; Kroeber, Markus W; Reiter, Andreas; Thomas, Susanne B; Hahn, Peter; Horch, Raymund E; Unglaub, Frank

    2010-03-01

    The objective of this study was to determine functional and subjective outcomes of an ulnar shortening procedure elected by patients who experienced persistent ulno-carpal symptoms following arthroscopic suture repair of a Palmer type 1B lesion. All patients had a dynamic ulna positive variance. Five patients (3 males and 2 females) with arthroscopic repair of Palmer type 1B tears who subsequently underwent ulnar shortening were reviewed. At the time of the arthroscopic repair the patients' average age was 37 +/- 13 years (range 16-52 years). Average time to follow-up was 14 +/- 6 months (range 10-23 months). The average age was 38 +/- 14 years (range 17-53 years) when the ulnar shortening was performed. The second follow-up took place 7 +/- 2 months (range 5-9 months) after ulnar shortening. During the follow-ups, range of motion, grip strength, pain, Modified Mayo Wrist Score, DASH Score, and ulnar length were evaluated. Citing persistent ulno-carpal symptoms, the patients elected ulnar shortening an average of 17 +/- 7months (range 13-29 months) following the arthroscopic repair. Prior to ulnar shortening the average static ulnar variance was 0.2 +/- 1.3 mm (range -1 to 2 mm), the average dynamic ulnar variance was 1.4 +/- 0.5 mm (range 1 to 2 mm). Ulnar shortening brought about further reduction in pain after the arthroscopic repair of the triangular fibrocartilage complex (TFCC) had already reduced it. As measured by a visual analogue scale, the average value after ulnar shortening was 2.2 +/- 2.1 (range 0.7-5.0). The average static ulnar variance was -3.4 +/- 2 mm (range -5 to -1 mm). Patients were very satisfied with the results of the ulnar shortening and four out of five indicated that it had significantly improved their symptoms and they would elect ulnar shortening again. Postoperative range of motion as a percentage of the contralateral side averaged 90% for the extension/flexion arc, 80% for the radial/ulnar deviation arc, and 100% for the pronation

  10. Structural limits on force production and shortening of smooth muscle.

    Science.gov (United States)

    Siegman, Marion J; Davidheiser, Sandra; Mooers, Susan U; Butler, Thomas M

    2013-02-01

    This study determined the factors that limit force production and shortening in two smooth muscles having very different relationships between active and passive force as a function of muscle length. The rat anococcygeus muscle develops active force over the range of lengths 0.2-2.0× the optimum length for force production (Lo). Passive tension due to extension of the resting muscle occurs only at lengths exceeding Lo. In contrast, the rabbit taenia coli develops force in the range of lengths 0.4-1.1 Lo, and passive force which is detectable at 0.56 Lo, increases to ~0.45 maximum active force at Lo, and increases sharply with further extension. The anococcygeus muscle can shorten to 0.2 Lo and the taenia coli to 0.4 Lo. Dynamic stiffness and energy usage at short muscle lengths suggest that the limit of shortening in the taenia coli, in contrast to the anococcygeus muscle, is not due to a failure of cross bridge interaction. Phosphorylation of the regulatory myosin light chains in intact muscles decreased to a small extent at short lengths compared to the decrease in force production. The differences in force production and the extent of shortening in the two muscles was maintained even when, following permeabilization, the myosin light chains were irreversibly phosphorylated with ATPγS, indicating that differences in activation played little, if any role. Ultrastructural studies on resting and activated muscles show that the taenia coli, which is rich in connective tissue (unlike the anococcygeus muscle) undergoes marked cellular twisting and contractile filament misalignment at short lengths with compression of the extracellular matrix. As a result, force is not transmitted in the longitudinal axis of the muscle, but is dissipated against an internal load provided by the compressed extracellular matrix. These observations on two very different normal smooth muscles reveal how differences in the relative contribution of active and passive structural elements

  11. Effects of fiber type on force depression after active shortening in skeletal muscle.

    Science.gov (United States)

    Joumaa, V; Power, G A; Hisey, B; Caicedo, A; Stutz, J; Herzog, W

    2015-07-16

    The aim of this study was to investigate force depression in Type I and Type II muscle fibers. Experiments were performed using skinned fibers from rabbit soleus and psoas muscles. Force depression was quantified after active fiber shortening from an average sarcomere length (SL) of 3.2µ m to an average SL of 2.6 µm at an absolute speed of 0.115f iber length/s and at a relative speed corresponding to 17% of the unloaded shortening velocity (V0) in each type of fibers. Force decay and mechanical work during shortening were also compared between fiber types. After mechanical testing, each fiber was subjected to myosin heavy chain (MHC) analysis in order to confirm its type (Type I expressing MHC I, and Type II expressing MHC IId). Type II fibers showed greater steady-state force depression after active shortening at a speed of 0.115 fiber length/s than Type I fibers (14.5±1.5% versus 7.8±1.7%). Moreover, at this absolute shortening speed, Type I fibers showed a significantly greater rate of force decay during shortening and produced less mechanical work than Type II fibers. When active shortening was performed at the same relative speed (17% V0), the difference in force depression between fiber types was abolished. These results suggest that no intrinsic differences were at the origin of the disparate force depressions observed in Type I and Type II fibers when actively shortened at the same absolute speed, but rather their distinct force-velocity relationships.

  12. Tetanic force potentiation of mouse fast muscle is shortening speed dependent.

    Science.gov (United States)

    Gittings, William; Huang, Jian; Vandenboom, Rene

    2012-10-01

    The activity dependent potentiation of peak isometric force associated with phosphorylation of the myosin regulatory light chain (RLC) is generally restricted to low activation frequencies. The purpose of this study was to determine if muscle shortening speed influenced the stimulus frequency domain over which concentric force potentiation was observed. To this end, mouse extensor digitorum longus (EDL) muscles (in vitro, 25 °C) were activated at a range of test frequencies (10, 25, 45, 70 or 100 Hz) during shortening ramps at 0.10, 0.30 or 0.50 of the maximal velocity of shortening (V(max)). This procedure was performed before and after a standard conditioning stimulus (CS) that elevated RLC phosphorylation from 0.08 ± 0.01 (rest) to 0.55 ± 0.01 (stimulated) moles phosphate per mol RLC, respectively (n = 9-11) (P shortening speed also increased the activation frequency at which concentric force potentiation was maximal, i.e. from 10 Hz at 0.10 V(max) to 10-25 and 25-45 Hz at 0.30 and 0.50 V(max), respectively. These results indicate that both the magnitude of and activation frequency dependence for concentric force potentiation of mouse EDL muscle is shortening speed dependent. Thus, muscle shortening speed may be a critical factor determining the functional utility of the myosin RLC phosphorylation mechanism.

  13. Ulnar or radial shortening osteotomy with a single saw cut.

    Science.gov (United States)

    Sraj, Shafic A; Budoff, Jeffrey E

    2009-09-01

    To determine which currently commercially available saw blades could be held at 45 degrees to the bone to reproducibly provide 2.0, 2.5, and 3.0 mm of ulna or radius shortening. Commercially available saw blades were tested for their ability to achieve the osseous shortening at a 45 degrees angle cut. When held at a 45 degrees angle to the bone, 2 Stryker 0.64-mm-thick blades achieved a mean shortening of 2.0 mm. A single Linvatec 1.2-mm-thick blade achieved a mean osseous shortening of 2.1 mm. Two Dyonics 0.65-mm-thick blades achieved a mean osseous shortening of 2.7 mm. Two Dyonics 0.89-mm-thick saw blades achieved a mean osseous shortening of 3.1 mm. Three Stryker 0.38-mm-thick saw blades mounted with the middle blade "upside down" with regard to the 2 outer blades achieved a mean osseous shortening of 3.2 mm. Two Linvatec 0.8-mm-thick saw blades achieved a mean osseous shortening of 3.1 mm. The findings of this study can help guide surgeons who desire to reproducibly shorten the ulna or radius by 2.0, 2.7, or 3.2 mm using a single saw cut to ensure a parallel osteotomy gap.

  14. Does a detection team shorten duration of untreated psychosis?

    DEFF Research Database (Denmark)

    Nordentoft, Merete; Thorup, Anne; Petersen, Lone;

    2008-01-01

    Duration of untreated psychosis (DUP) is shown to be associated with poor outcome in many domains. It has been shown that it is possible to shorten DUP when combining a detection team and an information campaign. The aim of this study was to evaluate whether DUP was shortened during the first 3 y...

  15. Instability during bunch shortening of an electron-cooled beam

    Directory of Open Access Journals (Sweden)

    M. Takanaka

    2003-10-01

    Full Text Available Bunch shortening causes an electron-cooled beam to be space charge dominated at low energies. Instability during the bunch shortening has been studied using a particle-tracking program where the 3D space-charge field due to the beam is calculated with a simplifying model.

  16. Shortening Tuberculosis Treatment With Fluoroquinolones: Lost in Translation?

    Science.gov (United States)

    Lanoix, Jean-Philippe; Chaisson, Richard E; Nuermberger, Eric L

    2016-02-15

    The disappointing recent failure of fluoroquinolone-containing regimens to shorten the duration of tuberculosis treatment in costly phase 3 trials has raised serious questions about the reliability of preclinical tuberculosis models, especially mice, and the current paradigm of regimen development. Therefore we re-examined data from murine models and early-stage clinical trials on which the pivotal trials were based, concluding that phase 3 trial results were in line with preceding studies. Finally, we offer suggestions for a more efficient and integrated preclinical and clinical regimen development program where quantitative pharmacokinetic and pharmacodynamic models more predictive of curative treatment durations are set forth. © The Author 2015. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail journals.permissions@oup.com.

  17. Cricket antennae shorten when bending (Acheta domesticus L.

    Directory of Open Access Journals (Sweden)

    Catherine eLoudon

    2014-06-01

    Full Text Available Insect antennae are important mechanosensory and chemosensory organs. Insect appendages, such as antennae, are encased in a cuticular exoskeleton and are thought to bend only between segments or subsegments where the cuticle is thinner, more flexible, or bent into a fold. There is a growing appreciation of the dominating influence of folds in the mechanical behavior of a structure, and the bending of cricket antennae was considered in this context. Antennae will bend or deflect in response to forces, and the resulting bending behavior will affect the sensory input of the antennae. In some cricket antennae, such as in those of Acheta domesticus, there are a large number (>100 of subsegments (flagellomeres that vary in their length. We evaluated whether these antennae bend only at the joints between flagellomeres, which has always been assumed but not tested. In addition we questioned whether an antenna undergoes a length change as it bends, which would result from some patterns of joint deformation. Measurements using light microscopy and SEM were conducted on both male and female adult crickets (Acheta domesticus with bending in four different directions: dorsal, ventral, medial and lateral. Bending occurred only at the joints between flagellomeres, and antennae shortened a comparable amount during bending, regardless of sex or bending direction. The cuticular folds separating antennal flagellomeres are not very deep, and therefore as an antenna bends, the convex side (in tension does not have a lot of slack cuticle to unfold and does not lengthen during bending. Simultaneously on the other side of the antenna, on the concave side in compression, there is an increasing overlap in the folded cuticle of the joints during bending. Antennal shortening during bending would prevent stretching of antennal nerves and may promote hemolymph exchange between the antenna and head.

  18. Systemic Lidocaine Shortens Length of Hospital Stay After Colorectal Surgery

    Science.gov (United States)

    Herroeder, Susanne; Pecher, Sabine; Schönherr, Marianne E.; Kaulitz, Grit; Hahnenkamp, Klaus; Friess, Helmut; Böttiger, Bernd W.; Bauer, Harry; Dijkgraaf, ↶oMarcel G. W.; Durieux, Marcel E.; Hollmann, Markus W.

    2007-01-01

    Objective: To characterize the beneficial effects of perioperative systemic lidocaine on length of hospital stay, gastrointestinal motility, and the inflammatory response after colorectal surgery. Summary Background Data: Surgery-induced stimulation of the inflammatory response plays a major role in the development of several postoperative disorders. Local anesthetics possess anti-inflammatory activity and are thought to positively affect patients' outcome after surgery. This double-blinded, randomized, and placebo-controlled trial aimed to evaluate beneficial effects of systemic lidocaine and to provide insights into underlying mechanisms. Methods: Sixty patients undergoing colorectal surgery, not willing or unable to receive an epidural catheter, were randomly assigned to lidocaine or placebo treatment. Before induction of general anesthesia, an intravenous lidocaine bolus (1.5 mg/kg) was administered followed by a continuous lidocaine infusion (2 mg/min) until 4 hours postoperatively. Length of hospital stay, gastrointestinal motility, and pain scores were recorded and plasma levels or expression of pro- and anti-inflammatory mediators determined. Results: Lidocaine significantly accelerated return of bowel function and shortened length of hospital stay by one day. No difference could be observed in daily pain ratings. Elevated plasma levels of IL-6, IL-8, complement C3a, and IL-1ra as well as expression of CD11b, l- and P-selectin, and platelet-leukocyte aggregates were significantly attenuated by systemic lidocaine. Conclusions: Perioperative intravenous lidocaine not only improved gastrointestinal motility but also shortened length of hospital stay significantly. Anti-inflammatory activity modulating the surgery-induced stress response may be one potential mechanism. Systemic lidocaine may thus provide a convenient and inexpensive approach to improve outcome for patients not suitable for epidural anesthesia. PMID:17667496

  19. Superimposed folding and thrusting by two phases of mutually orthogonal or oblique shortening in analogue models

    Science.gov (United States)

    Deng, Hongling; Koyi, Hemin A.; Nilfouroushan, Faramarz

    2016-02-01

    Orogens may suffer more than one phase shortening resulting in superposition of structures of different generations. Superimposition of orthogonal or oblique shortening is studied using sandbox and centrifuge modelling. Results of sand models show that in orthogonal superimposition, the two resulting structural trends are approximately orthogonal to each other. In oblique superimposition, structures trend obliquely to each other in the relatively thin areas of the model (foreland), and mutually orthogonal in areas where the model is thickened during the first phase of shortening (i.e. the hinterland). Thrusts formed during the first shortening phase may be reactivated during the later shortening phase. Spacing of the later phase structures is not as wide as expected, considering they across the pre-existing thickened wedge. Superposition of structures results in formation of type 1 fold interference pattern. Bedding is curved outwards both in the dome and basin structures. Folded layers are dipping and plunging outwards in a dome, while they are dipping and plunging inwards in a basin. In the areas between two adjacent domes or basins (i.e. where an anticline is superimposed by a syncline or a syncline is superimposed by an anticline), bedding is curved inwards, and the anticlines plunge inwards and the synclines outwards. The latter feature could be helpful to determine the age relationship for type 2 fold interference pattern. In tectonic regions where multiple phases of shortening have occurred, the orogenic-scale dome-and-basin and arrowhead-shaped interference patterns are commonly formed, as in the models. However, in some areas, the fold interference pattern might be modified by a later phase of thrusting. Similar to models results, superimposition of two and/or even more deformation phases may not be recorded by structures all over the tectonic area.

  20. Active shortening protects against stretch-induced force deficits in human skeletal muscle.

    Science.gov (United States)

    Saripalli, Anjali L; Sugg, Kristoffer B; Mendias, Christopher L; Brooks, Susan V; Claflin, Dennis R

    2017-02-23

    Skeletal muscle contraction results from molecular interactions of myosin "crossbridges" with adjacent actin filament binding sites. The binding of myosin to actin can be "weak" or "strong", and only strong binding states contribute to force production. During active shortening, the number of strongly-bound crossbridges declines with increasing shortening velocity. Forcibly stretching a muscle that is actively shortening at high velocity results in no apparent negative consequences whereas stretch of an isometrically (fixed-length) contracting muscle causes ultrastructural damage and a decline in force-generating capability. Our working hypothesis is that stretch-induced damage is uniquely attributable to the population of crossbridges that are strongly-bound. We tested the hypothesis that stretch-induced force deficits decline as the prevailing shortening velocity is increased. Experiments were performed on permeabilized segments of individual skeletal muscle fibers obtained from human subjects. Fibers were maximally activated and either allowed to generate maximum isometric force (Fo), or to shorten at velocities that resulted in force maintenance of ≈50% Fo or ≈2% Fo. For each test condition, a rapid stretch equivalent to 0.1 x optimal fiber length was applied. Relative to pre-stretch Fo, force deficits resulting from stretches applied during force maintenance of 100%, ≈50%, and ≈2% Fo were 23.2 ± 8.6%, 7.8 ± 4.2% and 0.3 ± 3.3%, respectively (mean ± SD, n=20). We conclude that stretch-induced damage declines with increasing shortening velocity, consistent with the working hypothesis that the fraction of strongly-bound crossbridges is a causative factor in the susceptibility of skeletal muscle to stretch-induced damage.

  1. A linear description of shortening induced changes in isometric length-force characteristics of rat muscle

    OpenAIRE

    Meijer, K; Grootenboer, H.J.; Koopman, H.F.J.M.; Huijing, P.A.

    1996-01-01

    Active muscle shortening reduces the isometric force potential of muscle. This observation indicates that the isometric length-force characteristics are altered during muscle shortening. Post-shortening decrease in isometric force depends on starting length, shortening amplitude and shortening velocity. In the present study, post-shortening decrease in isometric force was determined after isokinetic contractions with various shortening amplitudes initiated from different lengths of rat medial...

  2. Knowledge and attitudes of dentists toward shortened dental arch ...

    African Journals Online (AJOL)

    2015-08-03

    Aug 3, 2015 ... Key words: Attitude, dentist, knowledge, shortened dental arch. Date of Acceptance: ... practice of SDA therapy among dentists in Saudi Arabia, no studies are available in ..... partial denture was similar. Therefore, the attitude ...

  3. Distal femoral shortening in total hip arthroplasty for complex primary hip reconstruction. A new surgical technique.

    Science.gov (United States)

    Koulouvaris, Panagiotis; Stafylas, Kosmas; Sculco, Thomas; Xenakis, Theodore

    2008-10-01

    Successful total hip arthroplasty (THA) in congenital dislocated hips demands anatomical reduction in the normal center of rotation without overstretching the sciatic nerve and without excessive compression or abnormal forces across the joint. Proximal femoral and subtrochanteric shortening osteotomy has been described for THA for the treatment of dislocated hips. However, these osteotomies are demanding, associated with deformation of femoral canal and nonunion, and may increase the femoral stem stress. This study reports excellent results in 24 patients with a new surgical technique that combines THA with a distal femoral shortening in severely deformed hips using customized components.

  4. A comparison of transrectal hyperthermia, transurethral thermotherapy, urolume wallstent, and prostatic spiral for benign prostatic hyperplasia patients at poor operative risk.

    Science.gov (United States)

    Montorsi, F; Guazzoni, G; Bergamaschi, F; Consonni, P; Galli, L; Rigatti, P

    1994-01-01

    Transrectal hyperthermia, transurethral thermotherapy, prostatic stent, and prostatic spiral were used to treat 120 poor operative risk patients with symptomatic benign prostatic hyperplasia. The preoperative subjective and objective conditions of the four groups (each of 30 patients) were comparable. None of the patients had an indwelling catheter, but according to flow nomograms, all were obstructed. The greatest increase in peak flow rate was observed after stent placement, while the greatest decrease of residual urine volume was seen after the insertion of the stent and transrectal hyperthermia. According to maximum flow nomograms, only the placement of the stent resolved bladder outlet obstruction. The greatest improvement in subjective symptoms was the result of stent insertion, but the heating procedures also caused a significant reduction of symptom scores. The spiral produced satisfactory results only in the short term.

  5. A linear description of shortening induced changes in isometric length-force characteristics of rat muscle

    NARCIS (Netherlands)

    Meijer, K.; Grootenboer, H.J.; Koopman, H.F.J.M.; Huijing, P.A.

    1996-01-01

    Active muscle shortening reduces the isometric force potential of muscle. This observation indicates that the isometric length-force characteristics are altered during muscle shortening. Post-shortening decrease in isometric force depends on starting length, shortening amplitude and shortening veloc

  6. Edentulism and shortened dental arch in Brazilian elderly from the National Survey of Oral Health 2003

    Directory of Open Access Journals (Sweden)

    Marco Túlio Freitas Ribeiro

    2011-10-01

    Full Text Available OBJECTIVE: To describe the distribution of edentulism and estimate the prevalence of functional dentition and shortened dental arch among elderly population. METHODS: A population-based epidemiological study was carried out with a sample of 5,349 respondents aged 65 to 74 years obtained from the 2002 and 2003 Brazilian Ministry of Health/Division of Oral Health survey database. The following variables were studied: gender; macroregion of residence; missing teeth; percentage that met the World Health Organization goal for oral health in the age group 65 to 74 years (50% having at least 20 natural teeth; presence of shortened dental arch; number of posterior occluding pairs of teeth. The Chi-square test assessed the association between categorical variables. The Kruskal-Wallis and Mann-Whitney tests were used to assess differences of mean between number of posterior occluding pairs teeth, macro-region and gender. RESULTS: The elderly population had an average of 5.49 teeth (SD: 7.93 with a median of 0. The proportion of completely edentulous respondents was 54.7%. Complete edentulism was 18.2% in the upper arch and 1.9% in the lower arch. The World Health Organization goal was achieved in 10% of all respondents studied. However, only 2.7% had acceptable masticatory function and aesthetics (having at least shortened dental arch and a mean number of posterior occluding pairs of 6.94 (SD=2.97. There were significant differences of the percentage of respondents that met the World Health Organization goal and presence of shortened dental arch between men and women. There were differences in shortened dental arch between macroregions. CONCLUSIONS: The Brazilian epidemiological oral health survey showed high rate of edentulism and low rate of shortened dental arch in the elderly population studied, thus suggesting significant functional and aesthetic impairment in all Brazilian macroregions especially among women.

  7. Force depression following muscle shortening in sub-maximal voluntary contractions of human adductor pollicis.

    Science.gov (United States)

    Rousanoglou, Elissavet N; Oskouei, Ali E; Herzog, Walter

    2007-01-01

    Mechanical properties of skeletal muscles are often studied for controlled, electrically induced, maximal, or supra-maximal contractions. However, many mechanical properties, such as the force-length relationship and force enhancement following active muscle stretching, are quite different for maximal and sub-maximal, or electrically induced and voluntary contractions. Force depression, the loss of force observed following active muscle shortening, has been observed and is well documented for electrically induced and maximal voluntary contractions. Since sub-maximal voluntary contractions are arguably the most important for everyday movement analysis and for biomechanical models of skeletal muscle function, it is important to study force depression properties under these conditions. Therefore, the purpose of this study was to examine force depression following sub-maximal, voluntary contractions. Sets of isometric reference and isometric-shortening-isometric test contractions at 30% of maximal voluntary effort were performed with the adductor pollicis muscle. All reference and test contractions were executed by controlling force or activation using a feedback system. Test contractions included adductor pollicis shortening over 10 degrees, 20 degrees, and 30 degrees of thumb adduction. Force depression was assessed by comparing the steady-state isometric forces (activation control) or average electromyograms (EMGs) (force control) following active muscle shortening with those obtained in the corresponding isometric reference contractions. Force was decreased by 20% and average EMG was increased by 18% in the shortening test contractions compared to the isometric reference contractions. Furthermore, force depression was increased with increasing shortening amplitudes, and the relative magnitudes of force depression were similar to those found in electrically stimulated and maximal contractions. We conclude from these results that force depression occurs in sub

  8. Crustal shortening and Eocene extension in the southeastern Canadian Cordillera: Some thermal and rheological considerations

    Science.gov (United States)

    Liu, Mian; Furlong, Kevin P.

    1993-06-01

    Metamorphic core complexes in the southeastern Canadian Cordillera were formed during Eocene crustal extension, shortly (within a few millions of years) after Late Jurassic-Paleocene crustal shortening. Thermal-rheological modeling, constrained by geological and geochronological studies of the Valhalla core complex and other core complexes in this region, is used to investigate two major problems concerning the formation of these core complexes: (1) the dynamic links between crustal shortening and extension and (2) the cooling history and unroofing rates during extension. Thermal-rheological effects associated with crustal shortening are integrated through the history of crustal compression, since crustal shortening in this region was a long and slow process and cannot be treated as an instantaneous event. Our results suggest that crustal shortening may have played an important role in Eocene extension in the southeastern Canadian Cordillera by (1) producing a thickened and therefore unstable crust and (2) thermally weakening the lithosphere. However, heat generated by crustal shortening is not enough to account for the thermal state of the Valhalla complex, and additional heat sources at depth may be necessary. We then investigate thermal evolution during extension in both a simple shear model and a progressive pure stretching model. We show that the geotherm in an extensional region is time-and space-dependent and is affected by many variables including the preextensional thermal history and the mode of extension. Thus caution needs to be exercised when inferring unroofing rates from thermochronologic data. The cooling history of the Valhalla core complex may be explained by unroofing at rates of 1-2 mm/yr.

  9. Energy cost of isometric force production after active shortening in skinned muscle fibres.

    Science.gov (United States)

    Joumaa, V; Fitzowich, A; Herzog, W

    2017-02-23

    The steady state isometric force after active shortening of a skeletal muscle is lower than the purely isometric force at the corresponding length. This property of skeletal muscle is known as force depression. The purpose of this study was to investigate whether the energy cost of force production at the steady state after active shortening was reduced compared to the energy cost of force production for a purely isometric contraction performed at the corresponding length (same length, same activation). Experiments were performed in skinned fibres isolated from rabbit psoas muscle. Skinned fibres were actively shortened from an average sarcomere length of 3.0 µm to an average sarcomere length of 2.4 µm. Purely isometric reference contractions were performed at an average sarcomere length of 2.4 µm. Simultaneously with the force measurements, the ATP cost was measured during the last 30 seconds of isometric contractions using an enzyme-coupled assay. Stiffness was calculated during a quick stretch-release cycle of 0.2% fibre length performed once the steady state had been reached after active shortening and during the purely isometric reference contractions. Force and stiffness following active shortening were decreased by 10.0±1.8% and 11.0±2.2%, respectively compared to the isometric reference contractions. Similarly, ATPase activity per second (not normalized to the force) showed a decrease of 15.6±3.0% in the force depressed state compared to the purely isometric reference state. However, ATPase activity per second per unit of force was similar for the isometric contractions following active shortening (28.7±2.4 mM/mN.s.mm(3)) and the corresponding purely isometric reference contraction (30.9±2.8 mM/mN.s.mm(3)). Furthermore, the reduction in absolute ATPase activity per second was significantly correlated with force depression and stiffness depression. These results are in accordance with the idea that force depression following active shortening is

  10. [The concentration of growth factors in patients with inherent and acquired shortenings of limbs bones].

    Science.gov (United States)

    Strogov, M V; Luneva, S N; Novikov, K I

    2013-04-01

    The article deals with the results of study of level of growth factors in blood serum of patients with inherent and post-traumatic shortenings of limbs' bones. The detection in blood serum the level of epidermal growth factor insulin-like growth factor I and angiopoetins is proposed to monitor in given patients the reparative bone formation.

  11. USE AND SAFETY OF A SHORTENED HISTAMINE CHALLENGE TEST IN AN OCCUPATIONAL STUDY

    NARCIS (Netherlands)

    KREMER, AM; PAL, TM; OLDENZIEL, M; KERKHOF, M; DEMONCHY, JGR; RIJCKEN, B

    1995-01-01

    A shortened histamine challenge test was used in a study of occupational airway disease. We evaluated the safety, defined as the absence of a decrease in forced expiratory volume in one second (FEV1) of greater than 40%. The occurrence of complaints, the repeatability of test results, and the averag

  12. Shortening a Patient Experiences Survey for Medical Homes

    Directory of Open Access Journals (Sweden)

    Judy H. Ng

    2015-12-01

    Full Text Available The Consumer Assessment of Healthcare Providers and Systems—Patient-Centered Medical Home (CAHPS PCMH Survey assesses patient experiences reflecting domains of care related to general patient experience (access to care, communication with providers, office staff interaction, provider rating and PCMH-specific aspects of patient care (comprehensiveness of care, self-management support, shared decision making. The current work compares psychometric properties of the current survey and a proposed shortened version of the survey (from 52 to 26 adult survey items, from 66 to 31 child survey items. The revisions were based on initial psychometric analysis and stakeholder input regarding survey length concerns. A total of 268 practices voluntarily submitted adult surveys and 58 submitted child survey data to the National Committee for Quality Assurance in 2013. Mean unadjusted scores, practice-level item and composite reliability, and item-to-scale correlations were calculated. Results show that the shorter adult survey has lower reliability, but still it still meets general definitions of a sound survey for the adult version, and resulted in few changes to mean scores. The impact was more problematic for the pediatric version. Further testing is needed to investigate approaches to improving survey response and the relevance of survey items in informing quality improvement.

  13. Active myocyte shortening during the 'isovolumetric relaxation' phase of diastole is responsible for ventricular suction; 'systolic ventricular filling'.

    Science.gov (United States)

    Buckberg, Gerald D; Castellá, Manuel; Gharib, Morteza; Saleh, Saleh

    2006-04-01

    To study the 'isovolumetric relaxation' phase of rapid ventricular filling by analysis of the shortening of cardiac muscle in the endocardial and epicardial segments of the left ventricle in the dual helical model of the ventricular band, described by Torrent-Guasp. In 10 pigs (27-82 kg), temporal shortening by sonomicrometer crystals was recorded while recording ECG, and measuring intraventricular pressure and dP/dt with Millar pressure transducers. The following sequence was observed; shortening began in descending or endocardial segment, and 82+/-23 ms later it was initiated in the epicardial or ascending segment of the band. The descending segment stops shortening during the rapid filling phase of fast descent of ventricular pressure, but the ascending segment shortening continues for 92+/-33 ms, so that active shortening continues during the period of isovolumetric relaxation. During the rapid filling phase, dopamine decreased the interval between completion of endocardial and termination of epicardial contraction from 92+/-20 to 33+/-8 ms. Conversely propranolol delayed the start of epicardial shortening from 82+/-23 to 121+/-20 ms, and prolonged the duration of endocardial contraction, causing a closer (21+/-5 ms vs 92+/-20 ms) interval between termination of contraction of endocardial and epicardial fibers. The resultant slope of the rapid descent of the left ventricular pressure curve became prolonged. These time sequences show that ongoing unopposed ascending segment shortening occurs during the phase of rapid fall of ventricular pressure. These active shortening phases respond to positive and negative inotropic stimulation, and indicate the classic concept of 'isovolumetric relaxation', IVR, must be reconsidered, and the new term 'isovolumetric contraction', IVC, or systolic ventricular filing may be used.

  14. Pulse shortening of an ultrafast VECSEL

    Science.gov (United States)

    Waldburger, D.; Alfieri, C. G. E.; Link, S. M.; Gini, E.; Golling, M.; Mangold, M.; Tilma, B. W.; Keller, U.

    2016-03-01

    Ultrafast, optically pumped, passively modelocked vertical external-cavity surface-emitting lasers (VECSELs) are excellent sources for industrial and scientific applications that benefit from compact semiconductor based high-power ultrafast lasers with gigahertz repetition rates and excellent beam quality. Applications such as self-referenced frequency combs and multi-photon imaging require sub-200-fs pulse duration combined with high pulse peak power. Here, we present a semiconductor saturable absorber mirror (SESAM) modelocked VECSEL with a pulse duration of 147 fs and 328 W of pulse peak power. The average output power was 100 mW with a repetition rate of 1.82 GHz at a center wavelength of 1034 nm. The laser has optimal beam quality operating in a fundamental transverse mode with a M2 value of organic vapor phase epitaxy (MOVPE) with five pairs of strain-compensated InGaAs quantum wells (QWs). The QWs are placed symmetrical around the antinodes of the standing electric field at a reduced average field enhancement in the QWs of ≈ 0.5 (normalized to 4 outside the structure). These results overcome the trade-off between pulse duration and peak power of the state-of-the-art threshold values of 4.35 kW peak power for a pulse duration of 400 fs and 3.3 W peak power for a pulse duration of 107 fs.

  15. Chemical Sharpening, Shortening, and Unzipping of Boron Nitride Nanotubes

    Science.gov (United States)

    Liao, Yunlong; Chen, Zhongfang; Connell, John W.; Fay, Catharine C.; Park, Cheol; Kim, Jae-Woo; Lin, Yi

    2014-01-01

    Boron nitride nanotubes (BNNTs), the one-dimensional member of the boron nitride nanostructure family, are generally accepted to be highly inert to oxidative treatments and can only be covalently modifi ed by highly reactive species. Conversely, it is discovered that the BNNTs can be chemically dispersed and their morphology modifi ed by a relatively mild method: simply sonicating the nanotubes in aqueous ammonia solution. The dispersed nanotubes are significantly corroded, with end-caps removed, tips sharpened, and walls thinned. The sonication treatment in aqueous ammonia solution also removes amorphous BN impurities and shortened BNNTs, resembling various oxidative treatments of carbon nanotubes. Importantly, the majority of BNNTs are at least partially longitudinally cut, or "unzipped". Entangled and freestanding BN nanoribbons (BNNRs), resulting from the unzipping, are found to be approximately 5-20 nm in width and up to a few hundred nanometers in length. This is the fi rst chemical method to obtain BNNRs from BNNT unzipping. This method is not derived from known carbon nanotube unzipping strategies, but is unique to BNNTs because the use of aqueous ammonia solutions specifi cally targets the B-N bond network. This study may pave the way for convenient processing of BNNTs, previously thought to be highly inert, toward controlling their dispersion, purity, lengths, and electronic properties.

  16. Shortening spinal column reconstruction through posterior only approach for the treatment of unstable osteoporotic burst lumber fracture: a case report

    OpenAIRE

    Shawky, Ahmed; Kroeber, Markus

    2012-01-01

    Study design Case report. Clinical question This study reports if shortening reconstruction procedure through posterior approach only can be used in osteoporotic unstable fracture as well as post-traumatic burst fracture. Methods An 80-year-old female patient with unstable burst osteoporotic fracture of L1 underwent posterior approach corpectomy and shortening reconstruction of the spinal column by non-expandable cages. Result The surgery was uneventful, with average blood loss. Using of smal...

  17. Ulnar Shortening Osteotomy After Distal Radius Fracture Malunion: Review of Literature

    Science.gov (United States)

    Barbaric, Katarina; Rujevcan, Gordan; Labas, Marko; Delimar, Domagoj; Bicanic, Goran

    2015-01-01

    Malunion of distal radius fracture is often complicated with shortening of the radius with disturbed radio- ulnar variance, frequently associated with lesions of triangular fibrocartilage complex and instability of the distal radioulnar joint. Positive ulnar variance may result in wrist pain located in ulnar part of the joint, limited ulnar deviation and forearm rotation with development of degenerative changes due to the overloading that occurs between the ulnar head and corresponding carpus. Ulnar shortening osteotomy (USO) is the standard procedure for correcting positive ulnar variance. Goal of this procedure is to minimize the symptoms by restoring the neutral radio - ulnar variance. In this paper we present a variety of surgical techniques available for ulnar shorthening osteotomy, their advantages and drawbacks. Methods of ulnar shortening osteotomies are divided into intraarticular and extraarticular. Intraarticular method of ulnar shortening can be performed arthroscopically or through open approach. Extraarticular methods include subcapital osteotomy and osteotomy of ulnar diaphysis, which depending on shape can be transverse, oblique, and step cut. All of those osteotomies can be performed along wrist arthroscopy in order to dispose and treat possibly existing triangular fibrocartilage complex injuries. At the end we described surgical procedures that can be done in case of ulnar shorthening osteotomy failure. PMID:26157524

  18. Shortening full-length aptamer by crawling base deletion – Assisted by Mfold web server application

    Directory of Open Access Journals (Sweden)

    Subash C.B. Gopinath

    2017-06-01

    Full Text Available Systematic Evolution of Ligands by EXponential enrichment (SELEX is the method to select the specific aptamer against a wide range of targets. For this process, the initial library usually has a length of random sequences from ∼25 and it reaches over 100 bases. The lengthy sequences have disadvantages such as difficult to prepare, less stable and expensive. It is wise to prefer shorter version of aptamer for a wide range of applications including drug delivery process. It is a common practice to shorten the full-length aptamer by mapping analyses and it is tedious. Here, we used a crawling method to shorten the aptamer by different sequential deletion of bases from both 5′ and 3′ ends, assisted by Mfold web server application. Two different kinds of aptamer with varied lengths (randomized region of 30 and 74 bases were desired for this study, generated against Influenza A/Panama/2007/1999 (H3N2 and gD protein of Herpes Simplex Virus-1. It was found that shortening the aptamer length by crawling pattern is possible with the assistance of Mfold web server application. The obtained results resemble the shortened aptamer derived by mapping analyses. The proposed strategy is recommended to predict the shorter aptamer without involving any wet experimental section.

  19. Development and assessment of a shortened Quality of Life in Childhood Epilepsy Questionnaire (QOLCE-55).

    Science.gov (United States)

    Goodwin, Shane W; Lambrinos, Anastasia I; Ferro, Mark A; Sabaz, Mark; Speechley, Kathy N

    2015-06-01

    To develop and validate a shortened version of the Quality of Life in Childhood Epilepsy Questionnaire (QOLCE). A secondary aim was to compare baseline risk factors predicting health-related quality of life (HRQoL) in children newly diagnosed with epilepsy, as identified using the original and shortened version. Data came from the Health-Related Quality of Life in Children with Epilepsy Study (HERQULES, N = 373), a multicenter prospective cohort study. Principal component analysis reduced the number of items from the original QOLCE, and factor analysis was used to assess the factor structure of the shortened version. Convergent and divergent validity was assessed by correlating the Child Health Questionnaire (CHQ) with the shortened QOLCE. Multiple regression identified risk factors at diagnosis for HRQoL at 24 months. A four-factor, higher-order, 55-item solution was obtained. A total of 21 items were removed. The final model represents functioning in four dimensions of HRQoL: Cognitive, Emotional, Social, and Physical. The shortened QOLCE demonstrated acceptable fit: Bentler's Comparative Fit Index = 0.944; Tucker-Lewis Index = 0.942; root mean square approximation = 0.058 (90% CI: 0.056-0.061); weighted root mean square residuals (WRMR) = 1.582, and excellent internal consistency (α = 0.96, subscales α > 0.80). Factor loadings were good (first-order: λ = 0.66-0.93; higher-order λ = 0.66-0.85; p < 0.001 for all). The shortened QOLCE scores correlated strongly with similar subscales of the Child Health Questionnaire (ρ = 0.38-0.70) while correlating weakly with dissimilar subscales (ρ = 0.30-0.31). While controlling for HRQoL at diagnosis, predictors for better HRQoL at 24 months were the following: no cognitive problems reported (p = 0.001), better family functioning (p = 0.014), fewer family demands (p = 0.008), with an interaction between baseline HRQoL and cognitive problems (p = 0.011). Results offer initial evidence regarding reliability and validity

  20. GPS computer navigators to shorten EMS response and transport times.

    Science.gov (United States)

    Ota, F S; Muramatsu, R S; Yoshida, B H; Yamamoto, L G

    2001-05-01

    GPS (global positioning satellite system to determine one's position on earth) units have become inexpensive and compact. The purpose of this study is to assess the effectiveness of a GPS enhanced computer street map navigator to improve the ability of EMS drivers in an urban setting to locate their destination and shorten response times. For part I, residential addresses in the city were randomly selected from a telephone directory. Two driver/navigator teams were assigned to drive to the address adhering to speed limits. One team used a standard street map, whereas the other team used a GPS computer navigator. The travel time and distance of the runs were compared. For part II, the computer GPS navigator was placed on an ambulance to supplement their normal methods of navigation to find the address requesting EMS. After the run was completed, EMS providers were interviewed to determine their opinion of whether the GPS navigator was helpful. For part I the results showed that in the 29 initial test runs, comparing the GPS team versus the standard map team, the mean distances traveled were 8.7 versus 9.0 kilometers (not significant) and the mean travel times were 13.5 versus 14.6 minutes (P=.02), respectively. The GPS team arrived faster in 72% runs. For part II the results showed that most EMS providers surveyed noted that the GPS computer navigator enhanced their ability to find the destination and all EMS providers acknowledged that it would enhance their ability to find a destination in an area in which they were unfamiliar. These results suggest that a portable GPS computer navigator system is helpful and can enhance the ability of prehospital care providers to locate their destination. Because these units are accurate and inexpensive, GPS computer navigators may be a valuable tool in reducing pre-hospital transport times.

  1. Challenges in Shortening New Product Introduction in the Pharmaceutical Industry

    DEFF Research Database (Denmark)

    Hansen, Klaus Reinholdt Nyhuus; Grunow, Martin

    2010-01-01

    Drug developing companies are forced to utilize the effective protection of the patent by focusing on shortening the new product introduction [NPI] process measured as Time-to-Market [TTM]. Here the NPI process is considered and the trade-offs, which have to be address in the future are identifie...

  2. Telomere shortening reduces Alzheimer's disease amyloid pathology in mice

    NARCIS (Netherlands)

    Rolyan, Harshvardhan; Scheffold, Annika; Heinrich, Annette; Begus-Nahrmann, Yvonne; Langkopf, Britta Heike; Hoelter, Sabine M.; Vogt-Weisenhorn, Daniela M.; Liss, Birgit; Wurst, Wolfgang; Lie, Dieter Chichung; Thal, Dietmar Rudolf; Biber, Knut; Rudolph, Karl Lenhard

    Alzheimer's disease is a neurodegenerative disorder of the elderly and advancing age is the major risk factor for Alzheimer's disease development. Telomere shortening represents one of the molecular causes of ageing that limits the proliferative capacity of cells, including neural stem cells.

  3. A technique for nailing severely shortened and displaced tibia fractures.

    Science.gov (United States)

    Krause, Peter C; Whatley, Adam N; Mautner, James F

    2008-02-01

    Previously described techniques using external fixators or large distractors can simplify the closed nailing of tibia fractures and nonunions. However, delayed intramedullary nailing can be especially challenging when significant shortening or translation has occurred. We present a modification of an old technique for external fixator-assisted closed tibial nailing in these difficult cases.

  4. Cellular Consequences of Telomere Shortening in Histologically Normal Breast Tissues

    Science.gov (United States)

    2013-09-01

    in Figure 1, telomere shortening occurs specifically in luminal epithelial cells, but not in myoepithelial cells, in histologically normal terminal...the adjacent myoepithelial cells (panel A). In contrast, some TDLUs demonstrate dim telomere signals in the luminal cells when compared to the...adjacent myoepithelial cells (panel B). Through digital image analysis, quantitative determination of the telomere FISH signals confirms this moderated

  5. Telomere shortening reduces Alzheimer's disease amyloid pathology in mice

    NARCIS (Netherlands)

    Rolyan, Harshvardhan; Scheffold, Annika; Heinrich, Annette; Begus-Nahrmann, Yvonne; Langkopf, Britta Heike; Hoelter, Sabine M.; Vogt-Weisenhorn, Daniela M.; Liss, Birgit; Wurst, Wolfgang; Lie, Dieter Chichung; Thal, Dietmar Rudolf; Biber, Knut; Rudolph, Karl Lenhard

    2011-01-01

    Alzheimer's disease is a neurodegenerative disorder of the elderly and advancing age is the major risk factor for Alzheimer's disease development. Telomere shortening represents one of the molecular causes of ageing that limits the proliferative capacity of cells, including neural stem cells. Studie

  6. Oxidative Stress-induced Telomere Length Shortening of Circulating Leukocyte in Patients with Obstructive Sleep Apnea

    Science.gov (United States)

    Kim, Kyung Soo; Kwak, Jin Wook; Lim, Su Jin; Park, Yong Kyun; Yang, Hoon Shik; Kim, Hyun Jik

    2016-01-01

    The main mechanism of pathogenesis which causes systemic complications in obstructive sleep apnea (OSA) patients is believed to be intermittent hypoxia-induced intermediary effect and it depends on the burden of oxidative stress during sleep. We aimed to search the predictive markers which reflect the burden of systemic oxidative stress in patients with OSA and whether excessive telomere length shortening is a characteristic feature that can assess oxidative stress levels. We used quantitative PCR to measure telomere length using peripheral blood genomic DNA. Telomere lengths were compared in an age- and body mass index (BMI)-dependent manner in 34 healthy volunteers and 43 OSA subjects. We also performed reactive oxygen species assay to measure the concentration of hydrogen peroxide in the peripheral blood of healthy volunteers and OSA subjects. We found that the serum concentration of hydrogen peroxide was considerably higher in OSA patients, and that this was closely related with the severity of OSA. Significantly shortened telomere length was observed in the circulating leukocytes of the peripheral blood of OSA patients, and telomere length shortening was aggravated more acutely in an age- and BMI-dependent manner. An inverse correlation was observed between the concentration of hydrogen peroxide and the telomere length of OSA patients and excessive telomere length shortening was also linked to severity of OSA. The results provided evidence that telomere length shortening or excessive cellular aging might be distinctive in circulating leukocyte of OSA patients and may be an predictive biomarker for reflect the burden of oxidative stress in the peripheral blood of OSA patients. PMID:27699083

  7. Two-Stage Surgical Treatment for Non-Union of a Shortened Osteoporotic Femur

    Directory of Open Access Journals (Sweden)

    Galal Zaki Said

    2013-01-01

    Full Text Available Introduction: We report a case of non-union with severe shortening of the femur following diaphysectomy for chronic osteomyelitis.Case Presentation: A boy, aged 16 years presented with a dangling and excessively short left lower limb. He was using an elbow crutch in his right hand to help him walk. He had a history of diaphysectomy for chronic osteomyelitis at the age of 9. Examination revealed a freely mobile non-union of the left femur. The femur was the seat of an 18 cm shortening and a 4 cm defect at the non-union site; the knee joint was ankylosed in extension. The tibia and fibula were 10 cm short. Considering the extensive shortening in the femur and tibia in addition to osteoporosis, he was treated in two stages. In stage I, the femoral non-union was treated by open reduction, internal fixation and iliac bone grafting. The patient was then allowed to walk with full weight bearing in an extension brace for 7 months. In Stage II, equalization of leg length discrepancy (LLD was achieved by simultaneous distraction of the femur and tibia by unilateral frames. At the 6 month follow- up, he was fully weight bearing without any walking aid, with a heel lift to compensate the 1.5 cm shortening. Three years later he reported that he was satisfied with the result of treatment and was leading a normal life as a university student.Conclusions: Two-stage treatment succeeded to restore about 20 cm of the femoral shortening in a severely osteoporotic bone. It has also succeeded in reducing the treatment time of the external fixator.

  8. Enhanced muscle shortening and impaired Ca2+ channel function in an acute septic myopathy model.

    Science.gov (United States)

    Friedrich, Oliver; Hund, Ernst; von Wegner, Frederic

    2010-04-01

    Myopathies in critically ill patients are increasingly documented. Various animal models of chronic sepsis have been employed to investigate reduced membrane excitability or altered isometric contractility of skeletal muscle. In contrast, immediate changes occurring during acute sepsis are significantly under-characterised; L-type Ca(2+) channel function or isotonic shortening are examples. We recorded slowly activating L-type Ca(2+) currents (I (Ca)) in voltage-clamped single intact mouse skeletal muscle fibres and tested the effects of acute challenge with serum fractions from critical illness myopathy patients (CIM). Using a high-speed camera system, we simultaneously recorded unloaded fibre shortening during isotonic contractions with unprecedented temporal resolution (approximately 1,600 frames/s). Time courses of fibre lengths and shortening velocity were determined from automated imaging algorithms. CIM fractions acutely induced depression of I (Ca) amplitudes with no shifts in I (Ca)-V-relations. Voltage-dependent inactivation was unaltered and I (Ca) activation and inactivation kinetics were prolonged compared to controls. Unexpectedly, maximum unloaded speed of shortening was slightly faster following CIM serum applications, suggesting a direct action of CIM serum on weak-binding-state cross-bridges. Our results are compatible with a model where CIM serum might acutely reduce a fraction of functional L-type Ca(2+) channels and could account for reduced SR Ca(2+) release and force production in CIM patients. Acute increase in isotonic shortening velocity might be an early diagnostic feature suitable for testing in clinical studies. The acute challenge model is also robust against atrophy or fibre type changes that ordinarily would have to be considered in chronic sepsis models.

  9. Force depression and relaxation kinetics after active shortening and deactivation in mouse soleus muscle.

    Science.gov (United States)

    Van Noten, P; Van Leemputte, M

    2013-03-15

    After active shortening, isometric force production capacity of muscle is reduced (force depression, FD). The mechanism is incompletely understood but increasing cross-bridge detachment and/or decreasing attachment rate might be involved. Therefore we aimed to investigate the relation between work delivered during shortening (W), and change in half-relaxation time (Δ0.5RT) and change in the slow phase of muscle relaxation (Δkslow), considered as a marker for cross-bridge detachment rate, after shortening and after a short (0.7s) interruption of activation (deactivation). We hypothesized that shortening induces an accelerated relaxation related to W which is, similar to FD, largely abolished by a short deactivation. In 10 incubated supra-maximally stimulated mouse soleus muscles, we varied the amount of FD at L0 by varying shortening amplitude (0.6, 1.2 and 2.4mm). We found that W not only induces FD (R(2)=0.92) but also a dose dependent accelerated relaxation (R(2)=0.88 and R(2)=0.77 for respectively Δkslow and Δ0.5RT). In cyclic movements this is of functional significance, because the loss in force generating capacity might be (partially) compensated by faster relaxation. After a short deactivation, both FD and Δkslow were largely abolished but Δ0.5RT remained largely present. Under the assumption that Δkslow reflects a change in cross-bridge detachment rate, these results support the idea that FD is an intrinsic sarcomeric property originating from a work induced reduction of the number of force generating cross-bridges, however not via decreased attachment but via increased detachment rate. Copyright © 2013. Published by Elsevier Ltd.

  10. Temperature jump induced force generation in rabbit muscle fibres gets faster with shortening and shows a biphasic dependence on velocity.

    Science.gov (United States)

    Ranatunga, K W; Roots, H; Offer, G W

    2010-02-01

    We examined the tension responses to ramp shortening and rapid temperature jump (muscle fibres at 8-9 degrees C (the fibre length (L(0)) was approximately 1.5 mm and sarcomere length 2.5 microm). The aim was to investigate the strain sensitivity of crossbridge force generation in muscle. The T-jump induced tension rise was examined during steady shortening over a wide range of velocities (V) approaching the V(max) (V range approximately 0.01 to approximately 1.5 L(0) s(1)). In the isometric state, a T-jump induced a biphasic tension rise consisting of a fast (approximately 50 s(1), phase 2b) and a slow (approximately 10 s(1), phase 3) component, but if treated as monophasic the rate was approximately 20 s(1). During steady shortening the T-jump tension rise was monophasic; the rate of tension rise increased linearly with shortening velocity, and near V(max) it was approximately 200 s(1), approximately 10x faster than in the isometric state. Relative to the tension reached after the T-jump, the amplitude increased with shortening velocity, and near V(max) it was 4x larger than in the isometric state. Thus, the temperature sensitivity of muscle force is markedly increased with velocity during steady shortening, as found in steady state experiments. The rate of tension decline during ramp shortening also increased markedly with increase of velocity. The absolute amplitude of T-jump tension rise was larger than that in the isometric state at the low velocities (shortening velocity is increased, probably enhancement of crossbridge force generation and faster (post-stroke) crossbridge detachment by negative strain. Overall, our results show that T-jump force generation is strain sensitive and becomes considerably faster when exposed to negative strain. Thus the crossbridge force generation step in muscle is both temperature sensitive (endothermic) and strain sensitive.

  11. Circular smooth muscle contributes to esophageal shortening during peristalsis

    Institute of Scientific and Technical Information of China (English)

    Anil K Vegesna; Keng-Yu Chuang; Ramashesai Besetty; Steven J Phillips; Alan S Braverman; Mary F Barbe; Michael R Ruggieri

    2012-01-01

    AIM:To study the angle between the circular smooth muscle (CSM) and longitudinal smooth muscle (LSM) fibers in the distal esophagus.METHODS:In order to identify possible mechanisms for greater shortening in the distal compared to proximal esophagus during peristalsis,the angles between the LSM and CSM layers were measured in 9 cadavers.The outer longitudinal layer of the muscularis propria was exposed after stripping the outer serosa.The inner circular layer of the muscularis propria was then revealed after dissection of the esophageal mucosa and the underlying muscularis mucosa.Photographs of each specimen were taken with half of the open esophagus folded back showing both the outer longitudinal and inner circular muscle layers.Angles were measured every one cm for 10 cm proximal to the squamocolumnar junction (SCJ) by two independent investigators.Two human esophagi were obtained from organ transplant donors and the angles between the circular and longitudinal smooth muscle layers were measured using micro-computed tomography (micro CT) and Image J software.RESULTS:All data are presented as mean ± SE.The CSM to LSM angle at the SCJ and 1 cm proximal to SCJ on the autopsy specimens was 69.3 ± 4.62 degrees vs 74.9 ± 3.09 degrees,P =0.32.The CSM to LSM angle at SCJ were statistically significantly lower than at 2,3,4 and 5 cm proximal to the SCJ,69.3 ± 4.62 degrees vs 82.58 ± 1.34 degrees,84.04 ± 1.64 degrees,84.87 ± 1.04 degrees and 83.72 ± 1.42 degrees,P =0.013,P =0.008,P =0.004,P =0.009 respectively.The CSM to LSM angle at SCJ was also statistically significantly lower than the angles at 6,7 and 8 cm proximal to the SCJ,69.3 ± 4.62 degrees vs 80.18 ± 2.09 degrees,81.81 ± 1.75 degrees and 80.96 ± 2.04 degrees,P =0.05,P =0.02,P =0.03 respectively.The CSM to LSM angle at 1 cm proximal to SCJ was statistically significantly lower than at 3,4 and 5 cm proximal to the SCJ,74.94 ± 3.09 degrees vs 84.04 ± 1.64 degrees,84.87± 1.04 degrees and 83.72 ± 1

  12. Shortening induced effects on force (re)development in pig urinary smooth muscle

    NARCIS (Netherlands)

    E. van Asselt (Els); J.J.M. Pel (Johan); R. van Mastrigt (Ron)

    2007-01-01

    textabstractIntroduction: When muscle is allowed to shorten during an active contraction, the maximum force that redevelops after shortening is smaller than the isometric force at the same muscle length without prior shortening. We studied the course of force redevelopment after shortening in smooth

  13. Telomere shortening leads to earlier age of onset in ALS mice

    Science.gov (United States)

    Linkus, Birgit; Wiesner, Diana; MeΔner, Martina; Karabatsiakis, Alexander; Scheffold, Annika; Rudolph, K. Lenhard; Thal, Dietmar R.; Weishaupt, Jochen H.; Ludolph, Albert C.; Danzer, Karin M.

    2016-01-01

    Telomere shortening has been linked to a variety of neurodegenerative diseases. Recent evidence suggests that reduced telomerase expression results in shorter telomeres in leukocytes from sporadic patients with amyotrophic lateral sclerosis (ALS) compared with healthy controls. Here, we have characterized telomere length in microglia, astroglia and neurons in human post mortem brain tissue from ALS patients and healthy controls. Moreover, we studied the consequences of telomerase deletion in a genetic mouse model for ALS. We found a trend towards longer telomeres in microglia in the brains of ALS patients compared to non-neurologic controls. Knockout of telomerase leading to telomere shortening accelerated the ALS phenotype in SOD1G93A–transgenic mice. Our results suggest that telomerase dysfunction might contribute to the age-related risk for ALS. PMID:26978042

  14. Methods for Shortening and Extending the Carbon Chain in Carbohydrates

    DEFF Research Database (Denmark)

    Monrad, Rune Nygaard

    2008-01-01

    Carbohydrates play a central role in a variety of physiological and pathological processes such as HIV, cancer and diabetes. The understanding of these processes and the development of specific therapeutic agents is relying on the ability to chemically synthesize unnatural sugars, glycoconjugates...... and carbohydrate mimetics. Such polyhydroxylated compounds are conveniently synthesized from carbohydrates, however, due to the scarcity of many sugars from nature, efficient methods for transformation of readily available carbohydrates into valuable chiral building blocks are required. The work presented...... in this thesis focuses on the development and application of transition metal mediated methods for shortening and extending the carbon chain in carbohydrates thereby providing access to lower and higher sugars.A new catalytic procedure for shortening unprotected sugars by one carbon atom has been developed...

  15. [STT-arthrodesis versus radial shortening osteotomy for Kienböck's disease].

    Science.gov (United States)

    Das Gupta, K; Tünnerhoff, H G; Haussmann, P

    2003-10-01

    Thirteen patients treated by STT fusion for Kienböck's disease (five stage 3 a, seven stage 3 b, and one stage 4) and 36 patients treated by radial shortening osteotomy (20 stage 3 a, 16 stage 3 b) were checked after 26 and 83 months average. The active range of motion and grip strength were compared by means of the Cooney score, discomfort and pain by the DASH score. X-rays were compared for the bone structure of the lunate and development or progress of carpal collapse. After STT fusion the active over-all range of motion decreased by about 10 degrees, grip strength improved slightly by approximately 10 %, pain and discomfort improved well. Consolidation of the lunate was seen in ten of thirteen patients. The patients treated with radial shortening osteotomy could all improve their range of motion by about 10 degrees and the grip strength by about 20 %, whereas discomfort and pain persisted in some cases. X-rays showed consolidation of the lunate in cases of eleven patients stage 3 a and twelve patients stage 3 b. Radiological results were not always corresponding to the clinical results and the patients' subjective estimation. A group of eight patients could be followed up after more than ten years; all of them showed excellent results. This may indicate the long-term result for those patients who benefit from radius shortening with early pain relief. We recommend both procedures for stage 3 a and 3 b and suggest to decide from case to case, according to the needs of the patient. Ulna minus variance or the patient's wish to restore full active range of motion indicate radial shortening.

  16. Missing shortening in the thick-skinned retroarc thrust belt of the central Andes, northwestern Argentina, ~25°S

    Science.gov (United States)

    Pearson, D. M.; Kapp, P. A.; Decelles, P. G.; Reiners, P. W.

    2010-12-01

    A very large discrepancy exists among estimated retroarc shortening magnitudes in the thin-skinned thrust belt of Bolivia and the thick-skinned thrust belt of northwestern Argentina. Fieldwork and structural analysis from this study at ~25°S latitude in northwestern Argentina confirm the presence of a mainly west verging, thick-skinned style of shortening in the region which, taken together with thermochronological data ((U-Th)/He in zircon and apatite and published apatite fission track results), imply up to 10 km of rapid, Miocene exhumation. Although these results suggest that significant exhumation occurred in the region, displacements on mapped, discrete faults are insufficient in magnitude (by ~15-20%) to generate the observed ~58 km thick crust (Yuan et al., 2002). We suggest that additional, unrecognized shortening or crustal addition is required in the region to explain the thick crust and occurred by 1) crustal flow from neighboring regions of thickened crust; 2) passive roof thrusting whereby major, likely mid-crustal shortening was fed to higher structural levels; 3) tectonic underplating of trench or forearc rocks; and/or 4) significant penetrative strain not accommodated by through going faults. We do not discard other mechanisms of crustal thickening and/or addition in the region, but magnitudes of upper crustal shortening required by excess area calculations are best explained by penetrative deformation within rheologically weak metaturbidites of the Neoproterozoic to Cambrian Puncoviscana Formation. Although penetrative strain was generally not considered in cross section restorations in the retroarc of Bolivia, a relative lack of these rheologically weak rocks there probably precludes penetrative deformation as a significant mode of deformation and may partially reconcile the discrepancy in along-strike shortening magnitudes. Significant shortening has been accommodated by penetrative strain in other orogens worldwide, including western North

  17. Estimation of circumferential fiber shortening velocity by echocardiography.

    Science.gov (United States)

    Ruschhaupt, D G; Sodt, P C; Hutcheon, N A; Arcilla, R A

    1983-07-01

    The M-mode and two-dimensional echocardiograms of 40 young patients were analyzed to compare the mean circumferential fiber shortening velocity (Vcf) of the left ventricle calculated separately by two methods. The mean circumferential fiber shortening velocity was derived from the M-mode echocardiogram as minor axis shortening/ejection time and derived from the two-dimensional echocardiogram as actual circumference change/ejection time. With computer assistance, circumference was determined from the short-axis two-dimensional echocardiographic images during end-diastole and end-systole. Good correlations were obtained between the left ventricular diameter derived by M-mode echocardiography and the vertical axis during end-diastole (r = 0.79) and end-systole (r = 0.88) derived by two-dimensional echocardiography. Likewise, high correlations were noted between diameter and circumference in end-diastole (r = 0.89) and end-systole (r = 0.88). However, comparison of Vcf obtained by M-mode echocardiography with that obtained by two-dimensional echocardiography showed only fair correlation (r = 0.68). Moreover, the diameter/circumference ratio determined in end-diastole and end-systole differed significantly (p less than 0.001), possibly owing to the change in geometry of the ventricular sector image during systole. Although Vcf derived by M-mode echocardiography is a useful index of left ventricular performance, it does not truly reflect the circumference change during systole.

  18. Relationship between Spinal Cord Volume and Spinal Cord Injury due to Spinal Shortening.

    Directory of Open Access Journals (Sweden)

    Feng Qiu

    Full Text Available Vertebral column resection is associated with a risk of spinal cord injury. In the present study, using a goat model, we aimed to investigate the relationship between changes in spinal cord volume and spinal cord injury due to spinal shortening, and to quantify the spinal cord volume per 1-mm height in order to clarify a safe limit for shortening. Vertebral column resection was performed at T10 in 10 goats. The spinal cord was shortened until the somatosensory-evoked potential was decreased by 50% from the baseline amplitude or delayed by 10% relative to the baseline peak latency. A wake-up test was performed, and the goats were observed for two days postoperatively. Magnetic resonance imaging was used to measure the spinal cord volume, T10 height, disc height, osteotomy segment height, and spinal segment height pre- and postoperatively. Two of the 10 goats were excluded, and hence, only data from eight goats were analyzed. The somatosensory-evoked potential of these eight goats demonstrated meaningful changes. With regard to neurologic function, five and three goats were classified as Tarlov grades 5 and 4 at two days postoperatively. The mean shortening distance was 23.6 ± 1.51 mm, which correlated with the d-value (post-pre of the spinal cord volume per 1-mm height of the osteotomy segment (r = 0.95, p < 0.001 and with the height of the T10 body (r = 0.79, p = 0.02. The mean d-value (post-pre of the spinal cord volume per 1-mm height of the osteotomy segment was 142.87 ± 0.59 mm3 (range, 142.19-143.67 mm3. The limit for shortening was approximately 106% of the vertebral height. The mean volumes of the osteotomy and spinal segments did not significantly change after surgery (t = 0.310, p = 0.765 and t = 1.241, p = 0.255, respectively. Thus, our results indicate that the safe limit for shortening can be calculated using the change in spinal cord volume per 1-mm height.

  19. A randomized, double-blind, parallel-group, phase III study of shortening the dosing interval of subcutaneous tocilizumab monotherapy in patients with rheumatoid arthritis and an inadequate response to subcutaneous tocilizumab every other week: Results of the 12-week double-blind period.

    Science.gov (United States)

    Ogata, Atsushi; Tanaka, Yoshiya; Ishii, Tomonori; Kaneko, Motohide; Miwa, Hiroko; Ohsawa, Shino

    2017-06-16

    To determine the efficacy and safety of subcutaneous tocilizumab (TCZ-SC) monotherapy every week (qw) versus every other week (q2w) in patients with rheumatoid arthritis who had an inadequate response to TCZ-SC q2w. Adult patients in Japan with inadequate response to TCZ-SC q2w were randomized to either TCZ-SC 162 mg qw monotherapy or TCZ-SC 162 mg q2w monotherapy for 12 weeks (double-blind). The primary endpoint was the change from baseline in adjusted Disease Activity Score 28-erythrocyte sedimentation rate (DAS28-ESR) at week 12. Efficacy, safety and pharmacokinetics were assessed. TCZ-SC qw was superior to TCZ-SC q2w for adjusted mean change in DAS28-ESR from baseline to week 12. The difference in the change in DAS28-ESR between TCZ-SC qw and q2w was -1.21 (95%CI: -2.13, -0.30, p = .0108). A higher proportion of patients receiving TCZ-SC qw achieved DAS28-ESR remission/low disease activity than TCZ-SC q2w. Adverse events were 71.4% and 66.7% for TCZ-SC qw and q2w, respectively; infection was the most common event with one fatal case with TCZ-SC qw. In patients with inadequate response to TCZ-SC q2w, shortening the dosing interval to qw improved efficacy with acceptable tolerability. Occurrence of infection for both TCZ q2w and qw is important and needs careful attention.

  20. HULL GIRDER PROGRESSIVE COLLAPSE ANALYSIS USING IACS PRESCRIBED AND NLFEM DERIVED LOAD - END SHORTENING CURVES

    Directory of Open Access Journals (Sweden)

    Stanislav Kitarović

    2016-06-01

    Full Text Available This paper considers the hull girder ultimate strength of a bulk carrier at its midship section, as determined by an incremental-iterative progressive collapse analysis method prescribed by the International Association of Classification Societies Common Structural Rules for Bulk Carriers. In addition to the originally prescribed load – end shortening curves, curves determined by the nonlinear finite element method analysis (considering the influence of the idealized initial geometrical imperfections are also considered. Results obtained by both sets of curves are compared and discussed on both local (structural components load – end shortening curve and global (hull girder ulti-mate bending capacity and collapse sequence level, for both sagging and hogging cases.

  1. Does postsystolic motion or shortening predict recovery of myocardial function after primary percutanous coronary intervention?

    DEFF Research Database (Denmark)

    Terkelsen, Christian Juhl; Poulsen, Steen Hvitfeldt; Nørgaard, Bjarne Linde;

    2007-01-01

    or shortening appears more frequently in the acute phase in myocardial segments with impaired systolic function compared with normally functioning segments. However, presence of postsystolic contraction is not associated with improvement in strain or wall-motion score at follow-up, and does not seem......OBJECTIVE: The purpose of the study was to evaluate whether presence of postsystolic motion or shortening defined by Doppler tissue imaging may predict recovery of regional myocardial function in patients with ST-elevation myocardial infarction. METHODS: Echocardiography was performed a few hours......); and normal myocardial function in the acute phase (type E, n = 759). RESULTS: There were no differences among type A, B, C, and D segments with regard to the proportion presenting postsystolic tissue velocity equal to or greater than 1.0 cm/s (0.52, 0.54, 0.60, and 0.47, respectively, P = .20...

  2. Modulation of corticospinal excitability during lengthening and shortening contractions in the first dorsal interosseus muscle of humans.

    Science.gov (United States)

    Sekiguchi, Hirofumi; Kohno, Yutaka; Hirano, Tatsuya; Akai, Masami; Nakajima, Yasoichi; Nakazawa, Kimitaka

    2007-04-01

    Lengthening and shortening contractions are the fundamental patterns of muscle activation underlying various movements. It is still unknown whether or not there is a muscle-specific difference in such a fundamental pattern of muscle activation. The purpose of this study was, therefore, to investigate whether or not the relationship between lengthening and shortening contractions in the modulation of corticospinal excitability in the first dorsal interosseus (FDI) muscle is the same as that of previously tested muscles because the hand muscles are anatomically and functionally different from the other muscles. To this end, we investigated the relationship between the input-output curves of the corticospinal pathway (i.e., the relationship between the stimulus intensities vs. the area of motor-evoked potentials) during lengthening and shortening contractions in 17 healthy subjects. The shape of this relationship was sigmoidal and characterized by a plateau value, maximum slope, and threshold. The plateau value was at the same level between lengthening and shortening contractions. However, the maximum slope (P shortening contractions. These findings were different from the results of other muscles tested in previous studies (i.e., the soleus muscle and the elbow flexors). That is to say, the plateau value and the maximum slope during lengthening contractions were significantly lower than those during shortening contractions in previous studies. This study provides tentative evidence that the relationship between lengthening and shortening contractions in the modulation of corticospinal excitability differs between muscles, indicating that the underlying neural control is not necessarily the same even though the fundamental patterns of muscle activation are carried out.

  3. INFLUENCE OF SHORTENED AND LENGTHENED IMMOBILIZATION ON RAT SOLEUS MUSCLE ATROPHY

    Institute of Scientific and Technical Information of China (English)

    邢国刚; 樊小力; 吴苏娣; 宋新爱; 朱保恭; 唐斌

    2001-01-01

    Objective: To study the possible mechanism and prevention of disuse muscle atrophy. Methods: The shortened immobilization (plaster fixation) of rat' s soleus muscle (SOL) was used as the model of muscle and the lengthened immobilization of rat' s SOL muscle as "passive stretch" method. Types of skeletal muscle fibers were differentiated with m - ATPase staining technique. The changes of rat' s SOL muscle weight (wet weight) as well as the types and the mean cross - sectional area (CSA) of muscle fibers were examined respectively on day 2, 4,7, 14 and 21 under both shortened and lengthened immobilization and then the effect of passive stretch on soleus muscle atrophy in immobilized rats was observed. Results: When shortened immobilization was applied for 4 days, SOL muscle weight (wet weight) became lighter; the fiber crosssectional area (CSA) shrank and type Ⅰ muscle fibers started transforming into type Ⅱ, which all indicated immobilized muscles began to atrophy and as immobilization proceeded, muscle atrophy proceeded toward higher level. In contrast to that, when lengthened immobilization was applied, SOL muscle didn' t show any sign of atrophy until 7th day, and reached its highest level on day 14 and maintained that level even though immobilization continued. Conclusion: From the results, we conclude that passive stretch can either relieve or defer disuse muscle atrophy.

  4. Effective suppression of pulse shortening in a relativistic backward wave oscillator

    Science.gov (United States)

    Cao, Yibing; Song, Zhimin; Wu, Ping; Fan, Zhiqiang; Zhang, Yuchuan; Teng, Yan; Sun, Jun

    2017-03-01

    This paper discusses pulse shortening present in a C-band relativistic backward wave oscillator (RBWO). Effects of the collector plasma are believed to be the main cause. This viewpoint is first verified in numerical simulation. The simulation results show that light charged particles such as hydrogen ions in the collector plasma would axially enter into the beam-microwave interaction region and suppress high-power microwave (HPM) generation. Simultaneously, heavy charged particles such as oxygen or ferric ions in the collector plasma would radially expand out and change the end reflection of the RBWO. All these effects can result in pulse shortening. Simulations also demonstrate that a coaxial collector can effectively suppress plasma effects by retarding their axial and radial expansions. Furthermore, a HPM experiment has confirmed the validity of the coaxial collector. Using this structure, the output power of the RBWO has been increased from 2.5 GW to 3 GW. No pulse shortening has been observed in the HPM experiment.

  5. A Comparative Field Monitoring of Column Shortenings in Tall Buildings Using Wireless and Wired Sensor Network Systems

    Directory of Open Access Journals (Sweden)

    Sungho Lee

    2016-01-01

    Full Text Available A comparative field measurement for column shortening of tall buildings is presented in this study, with a focus on the reliability and stability of a wireless sensor network. A wireless sensor network was used for monitoring the column shortenings of a 58-story building under construction. The wireless sensor network, which was composed of sensor and master nodes, employed the ultra-high-frequency band and CDMA communication methods. To evaluate the reliability and stability of the wireless sensor network system, the column shortenings were also measured using a conventional wired monitoring system. Two vibration wire gauges were installed in each of the selected 7 columns and 3 walls. Measurements for selected columns and walls were collected for 270 days after casting of the concrete. The results measured by the wireless sensor network were compared with the results of the conventional method. The strains and column shortenings measured using both methods showed good agreement for all members. It was verified that the column shortenings of tall buildings could be monitored using the wireless sensor network system with its reliability and stability.

  6. Effect of Preactivation on Torque Enhancement by the Stretch-Shortening Cycle in Knee Extensors.

    Science.gov (United States)

    Fukutani, Atsuki; Misaki, Jun; Isaka, Tadao

    2016-01-01

    The stretch-shortening cycle is one of the most interesting topics in the field of sport sciences, because the performance of human movement is enhanced by the stretch-shortening cycle (eccentric contraction). The purpose of the present study was to examine whether the influence of preactivation on the torque enhancement by stretch-shortening cycle in knee extensors. Twelve men participated in this study. The following three conditions were conducted for knee extensors: (1) concentric contraction without preactivation (CON), (2) concentric contraction with eccentric preactivation (ECC), and (3) concentric contraction with isometric preactivation (ISO). Muscle contractions were evoked by electrical stimulation to discard the influence of neural activity. The range of motion of the knee joint was set from 80 to 140 degrees (full extension = 180 degrees). Angular velocities of the concentric and eccentric contractions were set at 180 and 90 degrees/s, respectively. In the concentric contraction phase, joint torques were recorded at 85, 95, and 105 degrees, and they were compared among the three conditions. In the early phase (85 degrees) of concentric contraction, the joint torque was larger in the ECC and ISO conditions than in the CON condition. However, these clear differences disappeared in the later phase (105 degrees) of concentric contraction. The results showed that joint torque was clearly different among the three conditions in the early phase whereas this difference disappeared in the later phase. Thus, preactivation, which is prominent in the early phase of contractions, plays an important role in torque enhancement by the stretch-shortening cycle in knee extensors.

  7. Variables Prognostic for Delayed Union and Nonunion Following Ulnar Shortening Fixed With a Dedicated Osteotomy Plate.

    Science.gov (United States)

    Gaspar, Michael P; Kane, Patrick M; Zohn, Ralph C; Buckley, Taylor; Jacoby, Sidney M; Shin, Eon K

    2016-02-01

    To examine potential risk factors for the development of delayed or nonunion following elective ulnar shortening osteotomy using a dedicated osteotomy plating system. We performed a retrospective review of all patients who underwent elective ulnar shortening using the TriMed single osteotomy dynamic compression plating system by 1 of 2 fellowship-trained hand surgeons over a 5-year period. Demographic data and medical, surgical, and social histories were reviewed. Time to bony union was determined radiographically by a blinded reviewer. Bivariate statistical analysis was performed to examine the effect of explanatory variables on the time to union and the incidence of delayed or nonunion. Those variables associated with the development of delayed or nonunion were used in a multivariate logistic regression model. Complications, including the need for additional surgery, were also recorded. Seventy-two ulnar shortening osteotomy procedures were performed in 69 patients. Delayed union, defined as ≥ 6 months to union, occurred in 8 of 72 cases (11%). Of 72 surgeries, 4 (6%) resulted in nonunions, all of which required additional surgery. Hardware removal was performed in 13 of 72 (18%) of the cases. Time to union was significantly increased in smokers (6 ± 3 months) versus nonsmokers (3 ± 1 months). On multivariable analysis, diabetics and active smokers demonstrated a significantly higher risk of developing delayed union or nonunion. Patient age, sex, body mass index, thyroid disease, worker's compensation status, alcohol use, and amount smoked daily did not have an effect on the time to union or the incidence of delayed or nonunion. Despite the use of an osteotomy-specific plating system, smokers and diabetics were at significantly higher risk for both delayed union and nonunion following elective ulnar shortening osteotomy. Other known risk factors for suboptimal bony healing were not found to have a deleterious effect. Copyright © 2016 American Society for

  8. Time course of isotonic shortening and the underlying contraction mechanism in airway smooth muscle.

    Science.gov (United States)

    Syyong, Harley T; Raqeeb, Abdul; Paré, Peter D; Seow, Chun Y

    2011-09-01

    Although the structure of the contractile unit in smooth muscle is poorly understood, some of the mechanical properties of the muscle suggest that a sliding-filament mechanism, similar to that in striated muscle, is also operative in smooth muscle. To test the applicability of this mechanism to smooth muscle function, we have constructed a mathematical model based on a hypothetical structure of the smooth muscle contractile unit: a side-polar myosin filament sandwiched by actin filaments, each attached to the equivalent of a Z disk. Model prediction of isotonic shortening as a function of time was compared with data from experiments using ovine tracheal smooth muscle. After equilibration and establishment of in situ length, the muscle was stimulated with ACh (100 μM) until force reached a plateau. The muscle was then allowed to shorten isotonically against various loads. From the experimental records, length-force and force-velocity relationships were obtained. Integration of the hyperbolic force-velocity relationship and the linear length-force relationship yielded an exponential function that approximated the time course of isotonic shortening generated by the modeled sliding-filament mechanism. However, to obtain an accurate fit, it was necessary to incorporate a viscoelastic element in series with the sliding-filament mechanism. The results suggest that a large portion of the shortening is due to filament sliding associated with muscle activation and that a small portion is due to continued deformation associated with an element that shows viscoelastic or power-law creep after a step change in force.

  9. Effect of Preactivation on Torque Enhancement by the Stretch-Shortening Cycle in Knee Extensors.

    Directory of Open Access Journals (Sweden)

    Atsuki Fukutani

    Full Text Available The stretch-shortening cycle is one of the most interesting topics in the field of sport sciences, because the performance of human movement is enhanced by the stretch-shortening cycle (eccentric contraction. The purpose of the present study was to examine whether the influence of preactivation on the torque enhancement by stretch-shortening cycle in knee extensors. Twelve men participated in this study. The following three conditions were conducted for knee extensors: (1 concentric contraction without preactivation (CON, (2 concentric contraction with eccentric preactivation (ECC, and (3 concentric contraction with isometric preactivation (ISO. Muscle contractions were evoked by electrical stimulation to discard the influence of neural activity. The range of motion of the knee joint was set from 80 to 140 degrees (full extension = 180 degrees. Angular velocities of the concentric and eccentric contractions were set at 180 and 90 degrees/s, respectively. In the concentric contraction phase, joint torques were recorded at 85, 95, and 105 degrees, and they were compared among the three conditions. In the early phase (85 degrees of concentric contraction, the joint torque was larger in the ECC and ISO conditions than in the CON condition. However, these clear differences disappeared in the later phase (105 degrees of concentric contraction. The results showed that joint torque was clearly different among the three conditions in the early phase whereas this difference disappeared in the later phase. Thus, preactivation, which is prominent in the early phase of contractions, plays an important role in torque enhancement by the stretch-shortening cycle in knee extensors.

  10. Extreme femoral shortening: an approach to the chronically dislocated hip in the nonambulatory pediatric population.

    Science.gov (United States)

    Kalawadia, Jay V; Patel, Ronak M; Jensen, Layne; Sarwark, John

    2014-09-01

    There are many surgical techniques for treating the chronically dislocated, painful hip in patients with neuromuscular spasticity, but each has significant complication rates. We conducted a study to examine the outcomes of a novel technique, an extreme varus femoral shortening osteotomy, used in nonambulatory patients with neuromuscular spasticity. Patients who underwent the procedure were identified retrospectively by surgical codes. Medical records were reviewed for range of motion, pain and functional assessment, surgical indications, complications, and results. In addition, preoperative and postoperative radiographs were assessed, and caretaker questionnaires reviewed. Between 2001 and 2010, 1 surgeon performed 6 femoral shortening osteotomies in 5 nonambulatory patients with neuromuscular spasticity. In all 5 cases, there were improvements in pain, sitting tolerance, ease of hygiene, and ease of transfers at a minimum follow-up of 2 years (mean, 3.4 years). Postoperative complications included asymptomatic heterotopic ossification and recurrent subluxation. Extreme femoral shortening is a reproducible surgical technique that alleviates pain and makes hygiene easier in nonambulatory children with symptomatic hip dislocations caused by neuromuscular spasticity. Our complication rate was comparable to that of other procedures.

  11. Field Monitoring of Column Shortenings in a High-Rise Building during Construction

    Directory of Open Access Journals (Sweden)

    Hyo Seon Park

    2013-10-01

    Full Text Available The automatic monitoring of shortenings of vertical members in high-rise buildings under construction is a challenging issue in the high-rise building construction field. In this study, a practical system for monitoring column shortening in a high-rise building under construction is presented. The proposed monitoring system comprises the following components: (1 a wireless sensing system and (2 the corresponding monitoring software. The wireless sensing system comprises the sensors and energy-efficient wireless sensing units (sensor nodes, master nodes, and repeater nodes, which automate the processes for measuring the strains of vertical members and transmitting the measured data to the remote server. The monitoring software enables construction administrators to monitor real-time data collected by the server via an Internet connection. The proposed monitoring system is applied to actual 66-floor and 72-floor high-rise buildings under construction. The system enables automatic and real-time measurements of the shortening of vertical members, which can result in more precise construction.

  12. Psychometric properties of a shortened version of the Physical Self-Concept Questionnaire (PSQ-S).

    Science.gov (United States)

    Rodríguez-Fernández, Arantzazu; Axpe, Inge; Goñi, Alfredo

    2015-01-01

    The four-dimensional model of physical self-concept which differentiates the physical self-perceptions of ability, condition, attractiveness and strength is widely accepted. In the last two decades much research has been done on the physical self-concept and its relations with the psychological well-being/distress, anxiety disorders or Eating Behavior Disorders (EBD). To validate a shortened version of the Physical Self-Concept Questionnaire (PSQ-S) and verify its ability to discriminate between people with different levels of EBD. Responses of 1478 subjects between 13 and 21 years old to the shortened version of the PSQ were analyzed in order to check indexes of reliability and validity. Furthermore, the scores of 96 women aged 14 to 23 years old diagnosed of EBD were compared to 96 others without clinical diagnosis. The results indicate a reliability of 0.93 and confirm the tetrafactorial structure of the physical selfconcept. The highest physical self-concept is that of those without a clinical diagnosis of EBD. The Shortened-PSQ is a simple, reliable and suitable screening tool both for educational and clinical settings. It also provides a sufficient measure of physical self-concept for research purposes.

  13. Decreasing initial telomere length in humans intergenerationally understates age-associated telomere shortening.

    Science.gov (United States)

    Holohan, Brody; De Meyer, Tim; Batten, Kimberly; Mangino, Massimo; Hunt, Steven C; Bekaert, Sofie; De Buyzere, Marc L; Rietzschel, Ernst R; Spector, Tim D; Wright, Woodring E; Shay, Jerry W

    2015-08-01

    Telomere length shortens with aging, and short telomeres have been linked to a wide variety of pathologies. Previous studies suggested a discrepancy in age-associated telomere shortening rate estimated by cross-sectional studies versus the rate measured in longitudinal studies, indicating a potential bias in cross-sectional estimates. Intergenerational changes in initial telomere length, such as that predicted by the previously described effect of a father's age at birth of his offspring (FAB), could explain the discrepancy in shortening rate measurements. We evaluated whether changes occur in initial telomere length over multiple generations in three large datasets and identified paternal birth year (PBY) as a variable that reconciles the difference between longitudinal and cross-sectional measurements. We also clarify the association between FAB and offspring telomere length, demonstrating that this effect is substantially larger than reported in the past. These results indicate the presence of a downward secular trend in telomere length at birth over generational time with potential public health implications.

  14. Effectiveness comparison of channel-assisted mini-incision and open Achilles shortening for treatment of healed Achilles tendon rupture

    Directory of Open Access Journals (Sweden)

    Hong-zhe QI

    2017-08-01

    Full Text Available Objective To compare the clinical effectiveness between the channel-assisted mini-invasion and open Achilles shortening for treatment of the elongated Achilles tendon following previous rupture. Methods The clinical data of 19 patients admitted from Dec. 2013 to Dec. 2015 and met the inclusion criteria were analyzed retrospectively. Eight patients were treated with shortening operation by channel-assisted minimally invasive repair system, while 11 patients received dissection of Krackow Achilles tendon shortening. There was no significant difference between the two groups in gender, age, injury to operation time, preoperative calf circumference and preoperative AOFAS (American Orthopaedic Foot & Ankle Society score (P>0.05. Results The operation time, incision length and postoperative hospital days were significantly less in min-invasion group than in incision group (P0.05. Conclusion Channel-assisted minimally invasive Achilles tendon shortening operation has not only similar effectiveness to the incision shorting operation for the treatment of elongated Achilles tendon following previous rupture, but also has the advantages of shortening operation time and stay in hospital and avoidance of sural nerve injury. DOI: 10.11855/j.issn.0577-7402.2017.07.12

  15. Comparison of EMG during passive stretching and shortening phases of each muscle for the investigation of parkinsonian rigidity.

    Science.gov (United States)

    Kwon, Yuri; Kim, Ji-Won; Kim, Ji-Sun; Koh, Seong-Beom; Eom, Gwang-Moon; Lim, Tae-Hong

    2015-01-01

    The aim of this study was to test the hypothesis in the literature that torque resistance of parkinsonian rigidity is the difference between the independent contributions of stretched and shortened muscles. The hypothesis was tested using muscle-specific stretch-shortening (MSSS) EMG ratio in this study. Nineteen patients with idiopathic Parkinson's disease (PD) and 18 healthy subjects (the mean age comparable to that of patients) participated in this study. The EMG activity was measured in the four muscles involved in wrist joint movement, i.e. flexor carpi radialis, flexor carpi ulnaris, extensor carpi radialis and extensor carpi ulnaris. The passive flexion-extension movement with a range of ±30∘ was applied at wrist joint. Root mean squared (RMS) mean was calculated from the envelope of the EMG for each of stretching and shortening phases. MSSS EMG ratio was defined as the ratio of RMS EMG of stretching phase and RMS EMG of shortening phase of a single muscle, and it was calculated for each muscle. MSSS EMG ratios were smaller than one in all muscles. These results indicate that all wrist muscles generate greater mean EMG during shortening than during stretching. Therefore, the torque resistance of parkinsonian rigidity cannot be explained as the simple summation of independent antagonistic torque pair.

  16. Stretch and shortening of skeletal muscles activated along the ascending limb of the force-length relation.

    Science.gov (United States)

    Rassier, Dilson E; Pun, Clara

    2010-01-01

    There is a history dependence of skeletal muscle contraction. When muscles are activated and subsequently stretched, they produce a long lasting force enhancement. When muscles are activated and subsequently shortened, they produce a long-lasting force depression. The purposes of the studies shown in this chapter were (1) to evaluate if force enhancement and force depression are present along the ascending limb of the force-length (FL) relation, (2) to evaluate if the history-dependent properties of force production are associated with sarcomere length (SL) non-uniformity, and (3) to determine the effects of cross-bridge (de)activation on force depression. Isolated myofibrils were activated by either Ca²(+) or MgADP and were subjected to consecutive stretches or shortenings along the ascending limb of the FL relation, separated by periods (approximately 5 s) of isometric contraction. Force after stretch was higher than force after shortening when the contractions were produced at similar SLs. The difference in force could not be explained by SL non-uniformity. After shortening, MgADP activation produced forces that were higher than Ca²(+) activation. Since MgADP induces the formation of strongly bound cross-bridges, the result suggests that force depression following shortening is associated with cross-bridge deactivation.

  17. Isolated lunotriquetral ligament tears treated with ulnar shortening osteotomy.

    Science.gov (United States)

    Mirza, Ather; Mirza, Justin B; Shin, Alexander Y; Lorenzana, Daniel J; Lee, Brian K; Izzo, Brett

    2013-08-01

    To evaluate outcomes in a single-surgeon series of ulnar shortening osteotomy for the treatment of traumatic isolated tears to the lunotriquetral interosseous ligament (LTIL). This study includes 53 consecutive cases of posttraumatic isolated LTIL tears treated with ulnar shortening osteotomy with minimum 1-year follow-up (range, 1.0-10.6 y). We confirmed all LTIL tears via arthroscopy before performing a precision 2.5-mm oblique osteotomy using a modified Rayhack technique. We assessed outcomes using grip strength measurements and Chun and Palmer's modified Gartland Werley wrist scoring system, which includes subjective and objective outcome measures. Preoperatively, 45 cases were graded as fair (28%; n = 15) or poor (57%; n = 30) on the modified Gartland Werley score. There were insufficient data to calculate grades in 8 cases (15%). At final follow-up, most patients exhibited excellent (51%; n = 27) or good (32%; n = 17) scores, some scored fair (17%; n = 9), and none scored as poor. All subjective and objective variables significantly improved over a mean follow-up of 36 months (range, 12-127 mo). Mean grip strength increased from a value of 23 kg before surgery to 33 kg over the same period, a 41% increase. All patients achieved clinical and radiographic union by 10 months. Osteotomy plates were removed routinely in most cases (89%; n = 47) at a mean of 17 months. Ulnar shortening osteotomy reduced symptoms of posttraumatic isolated LTIL tears in this single-surgeon series. Therapeutic IV. Copyright © 2013 American Society for Surgery of the Hand. Published by Elsevier Inc. All rights reserved.

  18. Kinetics of rate-dependent shortening of action potential duration in guinea-pig ventricle; effects of IK1 and IKr blockade.

    Science.gov (United States)

    Williams, B A; Dickenson, D R; Beatch, G N

    1999-03-01

    1. The kinetics of shortening of action potential duration (APD) following an increase in pacing rate, from 2 to 3.3 Hz, was characterized in guinea-pig ventricular preparations. Terikalant (RP62719), an inhibitor of the inwardly rectifying K+ current (IK1), and dofetilide, a specific inhibitor of the rapidly activating delayed-rectifier current (IKr), were applied to determine the effect of inhibition of these ion currents on slow APD shortening. 2. Action potentials were recorded from isolated guinea-pig ventricular myocytes using the perforated-patch patch-clamp technique, and monophasic action potentials were recorded from Langendorff-perfused guinea-pig ventricles using a contact epicardial probe. 3. Under control conditions, after an increase in pacing rate, APD immediately decreased, and then shortened slowly with an exponential time course. In ventricular myocytes, the time constant of this exponential shortening was 28+/-4 s and the amount of slow shortening was 21.9+/-0.9 ms (n=8) for an increase in rate from 2 to 3.3 Hz. Similar values were observed in Langendorff-perfused ventricles. 4. Terikalant dose-dependently increased APD and the increase was enhanced by rapid pacing ('positive' rate-dependence). The drug dose-dependently decreased the time constant of shortening and amount of slow APD shortening. In contrast, dofetilide, an inhibitor of IKr, which shows 'reverse' rate-dependent APD widening, had no significant effect on the time constant or amount of slow shortening. 5. These observations suggest that IK1 plays a role in rate-dependent shortening of APD. The results appear to support the hypothesis that 'reverse' rate-dependent effects of IKr blockers are due to these drugs not affecting the ion current(s) mediating intrinsic rate-dependent slow shortening of APD.

  19. The role of nonlinear viscoelasticity on the functionality of laminating shortenings

    Energy Technology Data Exchange (ETDEWEB)

    Macias-Rodriguez, Braulio A.; Peyronel, Fernanda; Marangoni, Alejandro G.

    2017-11-01

    The rheology of fats is essential for the development of homogeneous and continuous layered structures of doughs. Here, we define laminating shortenings in terms of rheological behavior displayed during linear-to-nonlinear shear deformations, investigated by large amplitude oscillatory shear rheology. Likewise, we associate the rheological behavior of the shortenings with structural length scales elucidated by ultra-small angle x-ray scattering and cryo-electron microscopy. Shortenings exhibited solid-like viscoelastic and viscoelastoplastic behaviors in the linear and nonlinear regimes respectively. In the nonlinear region, laminating shortenings dissipated more viscous energy (larger normalized dynamic viscosities) than a cake bakery shortening. The fat solid-like network of laminating shortening displayed a three-hierarchy structure and layered crystal aggregates, in comparison to two-hierarchy structure and spherical-like crystal aggregates of a cake shortening. We argue that the observed rheology, correlated to the structural network, is crucial for optimal laminating performance of shortenings.

  20. Simulation studies of circular muscle contraction, longitudinal muscle shortening, and their coordination in esophageal transport.

    Science.gov (United States)

    Kou, Wenjun; Pandolfino, John E; Kahrilas, Peter J; Patankar, Neelesh A

    2015-08-15

    On the basis of a fully coupled active musculomechanical model for esophageal transport, we aimed to find the roles of circular muscle (CM) contraction and longitudinal muscle (LM) shortening in esophageal transport, and the influence of their coordination. Two groups of studies were conducted using a computational model. In the first group, bolus transport with only CM contraction, only LM shortening, or both was simulated. Overall features and detailed information on pressure and the cross-sectional area (CSA) of mucosal and the two muscle layers were analyzed. In the second group, bolus transport with varying delay in CM contraction or LM shortening was simulated. The effect of delay on esophageal transport was studied. For cases showing abnormal transport, pressure and CSA were further analyzed. CM contraction by itself was sufficient to transport bolus, but LM shortening by itself was not. CM contraction decreased the CSA and the radius of the muscle layer locally, but LM shortening increased the CSA. Synchronized CM contraction and LM shortening led to overlapping of muscle CSA and pressure peaks. Advancing LM shortening adversely influenced bolus transport, whereas lagging LM shortening was irrelevant to bolus transport. In conclusion, CM contraction generates high squeezing pressure, which plays a primary role in esophageal transport. LM shortening increases muscle CSA, which helps to strengthen CM contraction. Advancing LM shortening decreases esophageal distensibility in the bolus region. Lagging LM shortening no longer helps esophageal transport. Synchronized CM contraction and LM shortening seems to be most effective for esophageal transport.

  1. Unloaded speed of shortening in voltage-clamped intact skeletal muscle fibers from wt, mdx, and transgenic minidystrophin mice using a novel high-speed acquisition system.

    Science.gov (United States)

    Friedrich, O; Weber, C; von Wegner, F; Chamberlain, J S; Fink, R H A

    2008-06-01

    Skeletal muscle unloaded shortening has been indirectly determined in the past. Here, we present a novel high-speed optical tracking technique that allows recording of unloaded shortening in single intact, voltage-clamped mammalian skeletal muscle fibers with 2-ms time resolution. L-type Ca(2+) currents were simultaneously recorded. The time course of shortening was biexponential: a fast initial phase, tau(1), and a slower successive phase, tau(2,) with activation energies of 59 kJ/mol and 47 kJ/mol. Maximum unloaded shortening speed, v(u,max), was faster than that derived using other techniques, e.g., approximately 14.0 L(0) s(-1) at 30 degrees C. Our technique also allowed direct determination of shortening acceleration. We applied our technique to single fibers from C57 wild-type, dystrophic mdx, and minidystrophin-expressing mice to test whether unloaded shortening was affected in the pathophysiological mechanism of Duchenne muscular dystrophy. v(u,max) and a(u,max) values were not significantly different in the three strains, whereas tau(1) and tau(2) were increased in mdx fibers. The results were complemented by myosin heavy and light chain (MLC) determinations that showed the same myosin heavy chain IIA profiles in the interossei muscles from the different strains. In mdx muscle, MLC-1f was significantly increased and MLC-2f and MLC-3f somewhat reduced. Fast initial active shortening seems almost unaffected in mdx muscle.

  2. Lactose tolerance test shortened to 30 minutes: an exploratory study of its feasibility and impact

    Directory of Open Access Journals (Sweden)

    José Luis Domínguez-Jiménez

    2014-06-01

    Full Text Available Introduction: Lactose malabsorption (LM is a very common condition with a high prevalence in our setting. Lactose tolerance test (LTT is a basic, affordable test for diagnosis that requires no complex technology. It has been recently shown that this test can be shortened to 3 measurements (baseline, 30 min, 60 min with no impact on final results. The purpose of our study was to assess the feasibility and benefits of LTT simplification and shortening to 30 min, as well as the financial impact entailed. Material and methods: A multicenter, observational study of consecutive patients undergoing LTT for LM suspicion. Patients received 50 g of lactose following a fasting period of 12 h, and had blood collected from a vein at all 3 time points for the measurement of blood glucose (mg/dl. Differences between the shortened and complete test forms were analyzed using McNemar's test. A comparison of blood glucose levels between patients with normal and abnormal results was performed using Student's T-test for independent mean values. Consistency was assessed using the kappa index. A p < 0.05 was considered to be statistically significant. Results: A total of 270 patients (69.6% females were included, with a mean age of 39.9 ± 16 years. LTT was abnormal for 151 patients (55.9%. We observed no statistically significant differences in baseline blood glucose levels between patients with normal and abnormal LTT results (p = 0.13; however, as was to be expected, such differences were obvious for the remaining time points (p < 0.01. Deleting blood glucose measurements at 60 minutes only led to overdiagnose LM (false positive results in 6 patients (2.22 %, with a kappa index of 0.95 (95% CI: 0.92-0.99 (p < 0.001 versus the complete test. Suppressing measurements at 60 min would have saved at least € 7,726. Conclusion: The shortening of LTT to only 2 measurements (baseline and 30-min hardly leads to any differences in final results, and would entail savings in

  3. Item response theory was used to shorten EORTC QLQ-C30 scales for use in palliative care

    NARCIS (Netherlands)

    M.A. Petersen; M. Groenvold; N. Aaronson; J. Blazeby; Y. Brandberg; A. de Graeff; P. Fayers; E. Hammerlid; M. Sprangers; G. Velikova; J.B. Bjorner

    2006-01-01

    Background and Objective: The goal was to develop a shortened version of the EORTC QLQ-C30 for use in palliative care. We wanted to keep as few items as possible in each scale while still being able to compare results with studies using the original scales. We examined the possibilities of shortenin

  4. AN IMPROVED OPTIMAL CHANNEL SHORTENING ALGORITHM FOR DMT MODULATION SYSTEMS

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    In Discrete Multi-Tone (DMT) modulation systems, the well-known technique to overcome the Inter-Carrier Interference (ICI)/Inter-Symbol Interference (ISI) caused by the inadequate Cyclic Prefix (CP) length is to use a Time-Domain Equalizer (TDE) at the receiver front-end. An algorithm used to calculate the coefficients of the optimal shortening Time Domain Equalizer (TDE) was given by Melsa. However, this al gorithm requires that the length of the TDE must be smaller than or equal to the memory length of the target impulse response. This paper modifies this algorithm and makes it not only fit for calculating the coefficients of the TDE with arbitrary length, but also have a much less computational time.

  5. [A shortened scale for overall, emotional and social loneliness].

    Science.gov (United States)

    de Jong Gierveld, J; van Tilburg, T

    2008-02-01

    Loneliness is an indicator of social well-being and pertains to the feeling of missing an intimate relationship (emotional loneliness) or missing a wider social network (social loneliness). The 11-item De Jong Gierveld scale has proved to be a valid and reliable measuring instrument for overall, emotional and social loneliness, although its length has sometimes rendered it difficult to use the scale in large surveys. In this study, we empirically tested a shortened version of the scale on data from two surveys (N=9448). Confirmatory factor analyses confirmed the specification of two latent factors. Congruent validity and the relationship with determinants (partner status, health) proved to be optimal. The 6-item De Jong Gierveld scale is a reliable and valid measuring instrument for overall, emotional and social loneliness, which is suitable for large surveys.

  6. Force depression decays during shortening in the medial gastrocnemius of the rat.

    Science.gov (United States)

    Till, Olaf; Siebert, Tobias; Blickhan, Reinhard

    2014-03-21

    Force depression due to shortening of activated skeletal muscles has previously been described to be long lasting during isometric contractions following the shortening. In the present study, using the medial gastrocnemius of the rat, effects of force depression have been made apparent during shortening by computationally partially compensating for the direct effect of shortening velocity due to the tension-velocity relation. Evidence was found for the decay and complete disappearance of force depression already during continuation of the shortening contraction to short muscle lengths.

  7. Effective shortening of picosecond pulses emitted by a YAG:Nd/sup 3 +/ laser

    Energy Technology Data Exchange (ETDEWEB)

    Dianov, E.M.; Karasik, A.Y.; Mamyshev, P.V.; Onishchukov, G.I.; Prokhorov, A.M.; Stel' makh, M.F.; Fomichev, A.A.

    1984-06-01

    A 15-fold reduction in the duration of YAG:Nd/sup 3 +/ laser pulses was achieved under conditions of active mode locking and Q switching at a pulse repetition frequency approx.1 kHz. Phase self-modulation in a single-mode quartz fiber waveguide of length 10 m resulted in broadening of the laser emission spectrum right up to approx.10 cm/sup -1/ at the waveguide exit. The pulses were then shortened in a system with a diffraction grating. The pulse duration was measured by a correlation system in which the second harmonic was generated.

  8. Cortical T2 signal shortening in amyotrophic lateral sclerosis is not due to iron deposits

    Energy Technology Data Exchange (ETDEWEB)

    Hecht, M.J.; Neundoerfer, B. [University of Erlangen-Nurenberg, Department of Neurology, Erlangen (Germany); Fellner, C.; Fellner, F.A. [University of Erlangen-Nurenberg, Institute of Diagnostic Radiology, Erlangen (Germany); Landes-Nervenklinik Wagner-Jauregg, Institute of Radiology, Linz (Austria); Schmid, A. [University of Erlangen-Nurenberg, Institute of Diagnostic Radiology, Erlangen (Germany)

    2005-11-01

    Signal shortening of the motor cortex in T2-weighted MR images is a frequent finding in patients with amyotrophic lateral sclerosis (ALS). The cause of signal shortening in ALS is unknown, although iron deposits have been suggested. To test this hypothesis, we acquired T2*-weighted gradient-echo (GRE) MR images in addition to T2-weighted turbo spin-echo in 69 patients with ALS. Signal shortening in T2-weighted images was found in 31 patients. In T2*-weighted GRE images, only three patients had signal shortening. One patient with additional bifrontal haemorrhage had frontal but no motor cortex signal shortening. Iron deposits do not cause cortical signal shortening in patients with ALS predominantly. Other factors are presumably more important in the generation of cortical T2 shortening in ALS. (orig.)

  9. Force generation examined by laser temperature-jumps in shortening and lengthening mammalian (rabbit psoas) muscle fibres.

    Science.gov (United States)

    Ranatunga, K W; Coupland, M E; Pinniger, G J; Roots, H; Offer, G W

    2007-11-15

    isometric muscle. Results are discussed in relation to the previous findings in isometric muscle fibres which showed that a T-jump promotes an early step in the crossbridge-ATPase cycle that generates force. In general, the finding that the T-jump effect on active muscle tension is pronounced during shortening, but is depressed/inhibited during lengthening, is consistent with the expectations from the Fenn effect that energy liberation (and acto-myosin ATPase rate) in muscle are increased during shortening and depressed/inhibited during lengthening.

  10. Developmental remodeling and shortening of the cardiac outflow tract involves myocyte programmed cell death.

    Science.gov (United States)

    Watanabe, M; Choudhry, A; Berlan, M; Singal, A; Siwik, E; Mohr, S; Fisher, S A

    1998-10-01

    The embryonic outflow tract is a simple tubular structure that connects the single primitive ventricle with the aortic sac and aortic arch arteries. This structure undergoes a complex sequence of morphogenetic processes to become the portion of the heart that aligns the right and left ventricles with the pulmonary artery and aorta. Abnormalities of the outflow tract are involved in many clinically significant congenital cardiac defects; however, the cellular and molecular processes governing the development of this important structure are incompletely understood. Histologic and tissue-tagging studies indicate that the outflow tract tissues compact and are incorporated predominantly into a region of the right ventricle. The hypothesis tested in the current study was that cell death or apoptosis in the muscular portion of the outflow tract is an important cellular mechanism for outflow tract shortening. The tubular outflow tract myocardium was specifically marked by infecting myocytes of the chicken embryo heart with a recombinant replication-defective adenovirus expressing beta-galactosidase (beta-gal) under the control of the cytomegalovirus promoter. Histochemical detection of the beta -gal-labeled outflow tract myocytes revealed that the tubular structure shortened to become a compact ring at the level of the pulmonic infundibulum over several days of development (stages 25-32, embryonic days 4-8). The appearance of apoptotic cardiomyocytes was correlated with OFT shortening by two histologic assays, TUNEL labeling of DNA fragments and AnnexinV binding. The rise and fall in the number of apoptotic myocytes detected by histologic analyses paralleled the change in activity levels of Caspase-3, a protease in the apoptotic cascade, measured in outflow tract homogenates. These results suggest that the elimination of myocytes by programmed cell death is one mechanism by which the outflow tract myocardium remodels to form the proper connection between the ventricular

  11. Mid-term clinical outcome of radial shortening for kienbock disease

    Directory of Open Access Journals (Sweden)

    Mohammad H Ebrahimzadeh

    2015-01-01

    Full Text Available Background: To evaluate the intermediate-term outcomes of radius shortening as a treatment for Kienbock′s disease. Materials and Methods: In a historical cohort, 16 skeletally mature patients (9 men and 7 women with Kienbock disease, who were treated with radial shortening osteotomy between 2002 and 2012, were reviewed in our study. The mean age of our patients was 30 (range 18-43 years old. According to Litchman staging, there were 7 wrists at stage II and 9 wrists at stage III (6 at stage IIIA and 3 at stage IIIB. The data of grip strength, pain (visual analog scale (VAS score, wrist range of motion (ROM, ulnar variance (according to Palmer method, and the Lichtman stage were gathered before and after surgery. We evaluated overall wrist function using the Mayo Wrist score and disabilities of the arm shoulder and hand (DASH score before surgery and at the last follow-up. Results: The average of follow-up was 7 years (range from 5 to 9 years. Preoperative ulnar variance was -1.3 mm (range from 2.5 to 1 preoperatively. The mean postoperative ulnar variance was 1 mm positive (range from 0.5 to 1.5. The VAS pain score, the mean arc of wrist flexion and extension, and grip strength improved significantly preoperatively compared to after recovery from surgery. The Lichtman stage was unchanged in nine patients, one grade worse in six patients, and one grade better in one patient. The mean DASH and Mayo scores improved significantly postoperatively compare with preoperation. Comparing preoperative positive, neuter, and negative ulnar variance, there was no significant difference in terms of VAS, DASH, and Mayo scores as well as ROM and grip strength. Conclusion: Our study shows that radius shortening surgery improves pain and disability regardless of ulnar variance.

  12. Varying mechanical coupling along the Andean margin: Implications for trench curvature, shortening and topography

    Science.gov (United States)

    Iaffaldano, G.; Di Giuseppe, E.; Corbi, F.; Funiciello, F.; Faccenna, C.; Bunge, H.-P.

    2012-03-01

    Convergent margins often exhibit spatial and temporal correlations between trench curvature, overriding plate shortening and topography uplift that provide insights into the dynamics of subduction. The Andean system, where the Nazca plate plunges beneath continental South America, is commonly regarded as the archetype of this class of tectonics systems. There is distinctive evidence that the degree of mechanical coupling between converging plates, i.e. the amount of resistive force mutually transmitted in the direction opposite to their motions, may be at the present-day significantly higher along the central Andean margin compared to the northern and southern limbs. However quantitative estimates of such resistance are still missing and would be desirable. Here we present laboratory models of subduction performed to investigate quantitatively how strong lateral coupling variations need to be to result in trench curvature, tectonic shortening and distribution of topography comparable to estimates from the Andean margin. The analogue of a two-layers Newtonian lithosphere/upper mantle system is established in a silicone putty/glucose syrup tank-model where lateral coupling variations along the interface between subducting and overriding plates are pre-imposed. Despite the simplicity of our setup, we estimate that coupling in the central margin as large as 20% of the driving force is sufficient to significantly inhibit the ability of the experimental overriding plate to slide above the subducting one. As a consequence, the central margin deforms and shortens more than elsewhere while the trench remains stationary, as opposed to the advancing lateral limbs. This causes the margin to evolve into a peculiar shape similar to the present-day trench of the Andean system.

  13. Contraction-specific differences in maximal muscle power during stretch-shortening cycle movements in elderly males and females

    DEFF Research Database (Denmark)

    Caserotti, Paolo; Aagaard, Per; Simonsen, Erik Bruun

    2001-01-01

    Aging, muscle power, stretch-shortening cycle, eccentric muscle actions, concentric contractions......Aging, muscle power, stretch-shortening cycle, eccentric muscle actions, concentric contractions...

  14. 3'UTR Shortening Potentiates MicroRNA-Based Repression of Pro-differentiation Genes in Proliferating Human Cells.

    Directory of Open Access Journals (Sweden)

    Yonit Hoffman

    2016-02-01

    Full Text Available Most mammalian genes often feature alternative polyadenylation (APA sites and hence diverse 3'UTR lengths. Proliferating cells were reported to favor APA sites that result in shorter 3'UTRs. One consequence of such shortening is escape of mRNAs from targeting by microRNAs (miRNAs whose binding sites are eliminated. Such a mechanism might provide proliferation-related genes with an expression gain during normal or cancerous proliferation. Notably, miRNA sites tend to be more active when located near both ends of the 3'UTR compared to those located more centrally. Accordingly, miRNA sites located near the center of the full 3'UTR might become more active upon 3'UTR shortening. To address this conjecture we performed 3' sequencing to determine the 3' ends of all human UTRs in several cell lines. Remarkably, we found that conserved miRNA binding sites are preferentially enriched immediately upstream to APA sites, and this enrichment is more prominent in pro-differentiation/anti-proliferative genes. Binding sites of the miR17-92 cluster, upregulated in rapidly proliferating cells, are particularly enriched just upstream to APA sites, presumably conferring stronger inhibitory activity upon shortening. Thus 3'UTR shortening appears not only to enable escape from inhibition of growth promoting genes but also to potentiate repression of anti-proliferative genes.

  15. Immediate liposuction could shorten the time for endoscopic axillary lymphadenectomy in breast cancer patients.

    Science.gov (United States)

    Shi, Fujun; Huang, Zonghai; Yu, Jinlong; Zhang, Pusheng; Deng, Jianwen; Zou, Linhan; Zhang, Cheng; Luo, Yunfeng

    2017-01-31

    Endoscopic axillary lymphadenectomy (EALND) was introduced to clinical work to reduce side effects of conventional axillary lymphadenectomy, while the lipolysis and liposuction of EALND made the process consume more time. The aim of the study was to determine whether immediate liposuction after tumescent solution injection to the axilla could shorten the total time of EALND. Fifty-nine patients were enrolled in the study, 30 of them received EALND with traditional liposuction method (TLM), and the rest 29 patients received EALND with immediate liposuction method (ILM). The operation time, cosmetic result, drainage amount, and hospitalization time of the two groups were compared. The median EALND operation time of TLM group and ILM group were 68 and 46 min, respectively, the difference was significant (P  0.05). Our work suggests immediate liposuction could shorten the endoscopic axillary lymphadenectomy process, and this method would not compromise the operation results. However, due to the limitations of the research, more work needs to be done to prove the availability and feasibility of immediate liposuction.

  16. The different muscle-energetics during shortening and stretch.

    Science.gov (United States)

    Jarosch, Robert

    2011-01-01

    The helical shape of the thin filaments causes their passive counterclockwise rotation during muscle stretch that increases tensile stress and torque at first by unwinding and then by winding up the four anchoring Z-filaments. This means storage of energy in the series elastic Z-filaments and a considerable decrease of the liberated energy of heat and work to (h-w(ap)), where h is the heat energy and w(ap) the stretch energy induced from outside by an apparatus. The steep thin filament helix with an inclination angle of 70° promotes the passive rotation during stretch, but impedes the smooth sliding of shortening by increased friction and production of frictional heat. The frictional heat may be produced by the contact with the myosin cross-bridges: (1) when they passively snap on drilling thin filaments from cleft to cleft over a distance 2 × 2.7 nm = 5.4 nm between the globular actin monomers in one groove, causing stepwise motion; or (2) when they passively cycle from one helical groove to the next (distance 36 nm). The latter causes more heat and may take place on rotating thin filaments without an effective forward drilling ("idle rotation"), e.g., when they produce "unexplained heat" at the beginning of an isometric tetanus. In an Appendix to this paper the different states of muscle are defined. The function of its most important components is described and rotation model and power-stroke model of muscular contraction is compared.

  17. Leg stiffness modulation during exhaustive stretch-shortening cycle exercise.

    Science.gov (United States)

    Kuitunen, S; Kyröläinen, H; Avela, J; Komi, P V

    2007-02-01

    The present study examined the effects of muscle activity modulation on leg stiffness during an exhaustive stretch-shortening cycle (SSC) exercise in eight male subjects. Reaction force, electromyography (EMG) of the soleus (Sol), gastrocnemius (Ga) and vastus lateralis (VL) muscles and sledge seat position were recorded during the SSC exercise, consisting of 100 maximal intermittent drop jumps followed by a continuous submaximal jumping until exhaustion, on a sledge apparatus. Metabolic loading was determined by measuring blood lactate (La). No change was found in leg stiffness during the maximal jumps, whereas the subsequent submaximal jumping induced a significant reduction by 27+/-12% (PEMG ratio between the braking and push-off phases in Sol (r=0.81, PEMG ratio at the end of the submaximal jumping in Sol (r=-0.88, Pmodulation between the braking and push-off phases in the triceps surae muscle, particularly in Ga, plays an important role in leg stiffness adjustments during fatiguing SSC exercise. It is suggested that efficient activity modulation (i.e. high EMG ratio) of the triceps surae muscle during an intensive fatiguing SSC exercise may postpone the exhaustion and development of metabolic fatigue.

  18. Treatment of tibial defect and bone nonunion with limb shortening with external fixator and reconstituted bone xenograft

    Institute of Scientific and Technical Information of China (English)

    王志刚; 刘建; 胡蕴玉; 孟国林; 金格勒; 袁志; 王海强; 戴先文

    2003-01-01

    Objective: To explore the effect of external fixator and reconstituted bone xenograft (RBX) in the treatment of tibial bone defect, tibial bone nonunion and congenital pseudarthrosis of the tibia with limb shortening. Methods: Twenty patients ( 13 males and 7 females) with tibial bone defect, tibial bone nonunion or congenital pseudarthrosis of the tibia with limb shortening were treated with external fixation. Two kinds of external fixators were used: a half ring sulcated external fixator used in 13 patients and a combined external fixator in 7 patients. Foot-drop was corrected at the same time with external fixation in 4 patients. The shortened length of the tibia was in the range of 2-9 cm, with an average of 4.8 cm. For bone grafting, RBX was used in 12 patients, autogenous ilium was used in 3 patients and autogenous fibula was implanted as a bone plug into the medullary canal in 1 case, and no bone graft was used in 4 patients. Results: All the 20 patients were followed-up for 8 months to 7 years, averaging 51 months. Satisfactory function of the affected extremities was obtained. All the shortened extremities were lengthened to the expected length. For all the lengthening area and the fracture sites, bone union was obtained at the last. The average healing time of 12 patients treated with RBX was 4.8 months. Conclusions: Both the half ring sulcated external fixator and the combined external fixator have the advantages of small trauma, simple operation, elastic fixation without stress shielding and non-limitation from local soft tissue conditions, and there is satisfactory functional recovery of affected extremities in the treatment of tibial bone defects, tibial bone nonunion and congenital pseudarthrosis of the tibia combined with limb shortening. RBX has good biocompatibility and does not cause immunological rejections. It can also be safely used in treatment of bone nonunion and has reliable effect to promote bone healing.

  19. Holocene internal shortening within the northwest Sub-Himalaya: Out-of-sequence faulting of the Jwalamukhi Thrust, India

    Science.gov (United States)

    Dey, Saptarshi; Thiede, Rasmus C.; Schildgen, Taylor F.; Wittmann, Hella; Bookhagen, Bodo; Scherler, Dirk; Strecker, Manfred R.

    2016-11-01

    The southernmost thrust of the Himalayan orogenic wedge that separates the foreland from the orogen, the Main Frontal Thrust, is thought to accommodate most of the ongoing crustal shortening in the Sub-Himalaya. Steepened longitudinal river profile segments, terrace offsets, and back-tilted fluvial terraces within the Kangra reentrant of the NW Sub-Himalaya suggest Holocene activity of the Jwalamukhi Thrust (JMT) and other thrust faults that may be associated with strain partitioning along the toe of the Himalayan wedge. To assess the shortening accommodated by the JMT, we combine morphometric terrain analyses with in situ 10Be-based surface-exposure dating of the deformed terraces. Incision into upper Pleistocene sediments within the Kangra Basin created two late Pleistocene terrace levels (T1 and T2). Subsequent early Holocene aggradation shortly before 10 ka was followed by episodic reincision, which created four cut-and-fill terrace levels, the oldest of which (T3) was formed at 10.1 ± 0.9 ka. A vertical offset of 44 ± 5 m of terrace T3 across the JMT indicates a shortening rate of 5.6 ± 0.8 to 7.5 ± 1.1 mm a-1 over the last 10 ka. This result suggests that thrusting along the JMT accommodates 40-60% of the total Sub-Himalayan shortening in the Kangra reentrant over the Holocene. We speculate that this out-of-sequence shortening may have been triggered or at least enhanced by late Pleistocene and Holocene erosion of sediments from the Kangra Basin.

  20. Exhumation and shortening distribution in the Taiwan orogen: insights from thermomechanical modeling

    Science.gov (United States)

    Mouthereau, F.; Yamato, P.; Burov, E.

    2007-12-01

    The Taiwan orogen has long been regarded as a case example for studying exhumation and erosion processes in association with mountain building. In the recent years, the increasing number of thermochronometric data (mainly ZFT and AFT ages) has allowed to better understanding the deep-seated tectonic processes. For instance, using thermomechanical wedge modelling, up to 50% of underplating has been proposed to explain the observed distribution of FT ages. So it would appear that a large amount of materials added to the orogen originated in more deeper and ductile parts of the crust. The nature of this additional flux (velocity, mechanism of deformation) is however poorly constrained in the current thermomechanical model. Our concern is to use a fully- coupled visco-elasto-plastic themomechanical numerical model to reproduce the observed FT ages and long- term distribution of shortening. To this aim we use the numerical code PARA(O)VOZ based on F.L.A.C. (Fast Lagrangian Analysis of Continua) algorithm. Our results show that the particular distribution of shortening across the Taiwan belt as well as the rapid exhumation and thermal conditions in the hinterland are well accounted for by two superimposed flows of upper and lower crustal rocks decoupled from the subducting Eurasian mantle.

  1. Hsp25 and Hsp72 content in rat skeletal muscle following controlled shortening and lengthening contractions.

    Science.gov (United States)

    Holwerda, Andrew M; Locke, Marius

    2014-12-01

    The cytoprotective proteins, Hsp25 and Hsp72, are increased in skeletal muscle after nondamaging, shortening contractions, but the temporal pattern of expression and stimulatory mechanisms remain unclear. Thus, we sought to define the in vivo temporal patterns of expression for Hsp25 and Hsp72 after 2 opposing contractions types. To do this, male Sprague-Dawley rats had 1 tibialis anterior (TA) muscle electrically stimulated (5 sets of 20 repetitions) while being either forcibly lengthened (LC) or shortened (SC). At 2, 8, 24, 48, 72, or 168 h after the contractions both the stimulated and the nonstimulated (contra-lateral control) TA muscles were removed and processed to examine muscle damage (hemotoxylin and eosin staining) and Hsp content (Western blot analyses). Cross-sections from TA muscles subjected to LCs showed muscle fibre damage at 8 h and thereafter. In contrast, no muscle fibre damage was observed at any time point following SCs. When normalized to contra-lateral controls, Hsp25 and Hsp72 content were significantly (P < 0.01) increased at 24 h (3.1- and 3.8-fold, respectively) and thereafter. There were no significant increases in Hsp25 or Hsp72 content at any time point following SC. These data suggest that LCs, but not SCs, result in Hsp accumulation and that the fibre/cellular damage sustained from LCs may be the stimulus for elevating Hsp content.

  2. Unloaded shortening velocity of voluntarily and electrically activated human dorsiflexor muscles in vivo.

    Science.gov (United States)

    Sasaki, Kazushige; Ishii, Naokata

    2010-09-27

    We have previously shown that unloaded shortening velocity (V(0)) of human plantar flexors can be determined in vivo, by applying the "slack test" to submaximal voluntary contractions (J Physiol 567:1047-1056, 2005). In the present study, to investigate the effect of motor unit recruitment pattern on V(0) of human muscle, we modified the slack test and applied this method to both voluntary and electrically elicited contractions of dorsiflexors. A series of quick releases (i.e., rapid ankle joint rotation driven by an electrical dynamometer) was applied to voluntarily activated dorsiflexor muscles at three different contraction intensities (15, 50, and 85% of maximal voluntary contraction; MVC). The quick-release trials were also performed on electrically activated dorsiflexor muscles, in which three stimulus conditions were used: submaximal (equal to 15%MVC) 50-Hz stimulation, supramaximal 50-Hz stimulation, and supramaximal 20-Hz stimulation. Modification of the slack test in vivo resulted in good reproducibility of V(0), with an intraclass correlation coefficient of 0.87 (95% confidence interval: 0.68-0.95). Regression analysis showed that V(0) of voluntarily activated dorsiflexor muscles significantly increased with increasing contraction intensity (R(2) = 0.52, Pmuscles remained unchanged (R(2)shortening velocity of human skeletal muscle in vivo.

  3. Moderate stem-cell telomere shortening rate postpones cancer onset in a stochastic model

    Science.gov (United States)

    Holbek, Simon; Bendtsen, Kristian Moss; Juul, Jeppe

    2013-10-01

    Mammalian cells are restricted from proliferating indefinitely. Telomeres at the end of each chromosome are shortened at cell division and when they reach a critical length, the cell will enter permanent cell cycle arrest—a state known as senescence. This mechanism is thought to be tumor suppressing, as it helps prevent precancerous cells from dividing uncontrollably. Stem cells express the enzyme telomerase, which elongates the telomeres, thereby postponing senescence. However, unlike germ cells and most types of cancer cells, stem cells only express telomerase at levels insufficient to fully maintain the length of their telomeres, leading to a slow decline in proliferation potential. It is not yet fully understood how this decline influences the risk of cancer and the longevity of the organism. We here develop a stochastic model to explore the role of telomere dynamics in relation to both senescence and cancer. The model describes the accumulation of cancerous mutations in a multicellular organism and creates a coherent theoretical framework for interpreting the results of several recent experiments on telomerase regulation. We demonstrate that the longest average cancer-free lifespan before cancer onset is obtained when stem cells start with relatively long telomeres that are shortened at a steady rate at cell division. Furthermore, the risk of cancer early in life can be reduced by having a short initial telomere length. Finally, our model suggests that evolution will favor a shorter than optimal average cancer-free lifespan in order to postpone cancer onset until late in life.

  4. Shortened telomere length in bipolar disorder: a comparison of the early and late stages of disease

    Directory of Open Access Journals (Sweden)

    Florencia M. Barbé-Tuana

    Full Text Available Objective: Bipolar disorder (BD has been associated with increased rates of age-related diseases, such as type II diabetes, metabolic syndrome, osteoporosis, and cardiovascular disorders. Several biological findings have been associated with age-related disorders, including increased oxidative stress, inflammation, and telomere shortening. The objective of this study was to compare telomere length among participants with BD at early and late stages and age- and gender-matched healthy controls. Methods: Twenty-six euthymic subjects with BD and 34 healthy controls were recruited. Genomic DNA was extracted from peripheral blood and mean telomere length was measured using real-time quantitative polymerase chain reaction. Results: Telomere length was significantly shorter in both the early and late subgroups of BD subjects when compared to the respective controls (p = 0.002 and p = 0.005, respectively. The sample size prevented additional subgroup analyses, including potential effects of medication, smoking status, and lifestyle. Conclusion: This study is concordant with previous evidence of telomere shortening in BD, in both early and late stages of the disorder, and supports the notion of accelerated aging in BD.

  5. Semi-automated detection of fractional shortening in zebrafish embryo heart videos

    Directory of Open Access Journals (Sweden)

    Nasrat Sara

    2016-09-01

    Full Text Available Quantifying cardiac functions in model organisms like embryonic zebrafish is of high importance in small molecule screens for new therapeutic compounds. One relevant cardiac parameter is the fractional shortening (FS. A method for semi-automatic quantification of FS in video recordings of zebrafish embryo hearts is presented. The software provides automated visual information about the end-systolic and end-diastolic stages of the heart by displaying corresponding colored lines into a Motion-mode display. After manually marking the ventricle diameters in frames of end-systolic and end-diastolic stages, the FS is calculated. The software was evaluated by comparing the results of the determination of FS with results obtained from another established method. Correlations of 0.96 < r < 0.99 between the two methods were found indicating that the new software provides comparable results for the determination of the FS.

  6. Dynamic Route Shortening and Route Repairing Mechanism for Mobile Ad Hoc Networks

    Directory of Open Access Journals (Sweden)

    T. R. Rangaswamy

    2012-01-01

    -NONE X-NONE MicrosoftInternetExplorer4 /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0in 5.4pt 0in 5.4pt; mso-para-margin:0in; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;} ‘‘optimal’’ alternative route. Results: Our protocol performs better than the original (AODV, in terms of the packet delivery rate, average hops and packet loss. Conclusion: Our proposed routing protocol constructs the alternative route with the optimal route in the Dynamic Route Shortening scheme when there is a link failure between any two nodes.

  7. Shortened telomere length is associated with increased risk of cancer: a meta-analysis.

    Directory of Open Access Journals (Sweden)

    Hongxia Ma

    Full Text Available BACKGROUND: Telomeres play a key role in the maintenance of chromosome integrity and stability, and telomere shortening is involved in initiation and progression of malignancies. A series of epidemiological studies have examined the association between shortened telomeres and risk of cancers, but the findings remain conflicting. METHODS: A dataset composed of 11,255 cases and 13,101 controls from 21 publications was included in a meta-analysis to evaluate the association between overall cancer risk or cancer-specific risk and the relative telomere length. Heterogeneity among studies and their publication bias were further assessed by the χ(2-based Q statistic test and Egger's test, respectively. RESULTS: The results showed that shorter telomeres were significantly associated with cancer risk (OR = 1.35, 95% CI = 1.14-1.60, compared with longer telomeres. In the stratified analysis by tumor type, the association remained significant in subgroups of bladder cancer (OR = 1.84, 95% CI = 1.38-2.44, lung cancer (OR = 2.39, 95% CI = 1.18-4.88, smoking-related cancers (OR = 2.25, 95% CI = 1.83-2.78, cancers in the digestive system (OR = 1.69, 95% CI = 1.53-1.87 and the urogenital system (OR = 1.73, 95% CI = 1.12-2.67. Furthermore, the results also indicated that the association between the relative telomere length and overall cancer risk was statistically significant in studies of Caucasian subjects, Asian subjects, retrospective designs, hospital-based controls and smaller sample sizes. Funnel plot and Egger's test suggested that there was no publication bias in the current meta-analysis (P = 0.532. CONCLUSIONS: The results of this meta-analysis suggest that the presence of shortened telomeres may be a marker for susceptibility to human cancer, but single larger, well-design prospective studies are warranted to confirm these findings.

  8. Shortening-induced torque depression in old men: implications for age-related power loss.

    Science.gov (United States)

    Power, Geoffrey A; Makrakos, Demetri P; Stevens, Daniel E; Herzog, Walter; Rice, Charles L; Vandervoort, Anthony A

    2014-09-01

    Following active muscle shortening, the steady-state isometric torque at the final muscle length is lower than the steady-state torque obtained for a purely isometric contraction at that same final muscle length. This well-documented property of skeletal muscle is termed shortening-induced torque depression (TD). Despite many investigations into the mechanisms of weakness and power loss in old age, the influence of muscle shortening on the history dependence of isometric torque production remains to be elucidated. Thus, it is unclear whether older adults are disadvantaged for torque and power production following a dynamic shortening contraction. The purpose of this study was to evaluate shortening-induced TD in older adults, and to determine whether shortening-induced TD is related to power loss. Maximal voluntary isometric dorsiflexion contractions (MVC; 10s) in 8 young (25.5±3.7years) and 9 old (76.1±5.4years) men were performed on a HUMAC NORM dynamometer as a reference, and then again following an active shortening of 40° joint excursion (40°PF-0°PF) at angular velocities of 15°/s and 120°/s. Work and instantaneous power were derived during shortening. Shortening-induced TD was calculated and expressed as a percentage by determining the mean torque value over 1s during the isometric steady state of the MVC following shortening, divided by the mean torque value for the same 1s time period during the isometric reference MVC. To assess muscle activation, electromyography (root mean square; EMGRMS) of the tibialis anterior (TA) and soleus (SOL) was calculated at identical time points used in assessing shortening-induced TD, and voluntary activation (VA) was assessed using the interpolated twitch technique. Old were 18% weaker than young for MVC, and ~40% less powerful for 15°/s and 120°/s of shortening. Old produced 37% and 21% less work for 15°/s and 120°/s than young, respectively. Furthermore, old experienced 60% and 70% greater shortening-induced TD

  9. Prediction of peak forces for a shortening smooth muscle tissue subjected to vibration.

    Science.gov (United States)

    Pidaparti, Ramana M; Dhanaraj, Nandhini; Meiss, Richard A

    2008-01-01

    The objective of the present study is to investigate the peak forces for a tracheal smooth muscle tissue subjected to an applied longitudinal vibration following isotonic shortening. A non-linear finite element analysis was carried out to simulate the vibratory response under experimental conditions that corresponds to forced length oscillations at 33 Hz for 1 second. The stiffness change and hysteresis estimated from the experimental data was used in the analysis. The finite element results of peak forces are compared to the experimental data obtained. The comparison of results indicate that the approach and the vibratory response obtained may be useful for describing the cross-bridge de-attachments within the cells as well as connective tissue connections characteristic of tracheal smooth muscle tissue.

  10. Limb shortening in the course of solitary bone cyst treatment - a comparative study

    Energy Technology Data Exchange (ETDEWEB)

    Glowacki, Maciej [Karol Marcinkowski University of Medical Sciences, Department of Paediatric Orthopaedics, Poznan (Poland); Ignys-O' Byrne, Anna [J. Strus City Hospital, Department of Radiology, Poznan (Poland); Ignys, Iwona [Karol Marcinkowski University of Medical Sciences, Department of Paediatric Gastroenterology and Metabolic Diseases, Poznan (Poland); Wroblewska, Katarzyna [Karol Marcinkowski University of Medical Sciences, Department of Radiology, Poznan (Poland)

    2011-02-15

    The aim of this paper is to evaluate the frequency of limb shortening in the course of solitary bone cyst treatment. The correlation between the mode of treatment as well as the occurrence of pathological fracture, cyst location, volume, and locularity were examined. A retrospective analysis was carried out on 135 patients where 80 underwent curettage and bone grafting and 55 were administered methylprednisolone injection with a mean time to follow-up of 12 years. Based on clinical and radiological evaluation, limb shortening was found in ten patients when the data before and after treatment was compared. Limb shortening ranging from 1 to 5 cm during the course of the treatment was observed: six in humerus, two in femur, two in tibia. Those with epiphyseal changes, magnetic resonance imaging to evaluate the degree of growth plate damage was performed. Patients with and without limb shortening did not differ statistically regarding the applied method of treatment. The cyst volume was significantly larger in the group of patients with limb shortening when compared to the group of patients with no limb shortening. In patients treated with curettage and bone grafting, the mode of treatment does not increase the frequency of occurrence of iatrogenic limb shortening. In patients with limb shortening, a statistically significant larger volume of the cyst was observed. (orig.)

  11. Compatibility of selected plant-based shortening as lard substitute: microstructure, polymorphic forms and textural properties

    Directory of Open Access Journals (Sweden)

    N. A.M. Yanty

    2017-03-01

    Full Text Available A study was carried out to determine the compatibility of three plant-based shortening mixtures to lard shortening (LD in terms of microstructure, polymorphic forms, and textural properties. The shortenings of binary, ternary, and quaternary fat mixtures were prepared according to a standard procedure by blending mee fat (MF with palm stearin (PS in a 99:1 (w/w ratio; avocado fat (Avo with PS and cocoa butter (CB in a 84:7:9 (w/w ratio; palm oil (PO with PS, soybean oil (SBO and CB in a 38:5:52:5 (w/w ratio, respectively. The triacylglycerol composition, polymorphic forms, crystal morphology, and textural properties of the shortening were evaluated. This study found that all three plant-based shortenings and LD shortening were similar with respect to their consistency, hardness and compression and adhesiveness values. However, all plant-based shortening was found to be dissimilar to LD shortening with respect to microstructure.

  12. Assisting High School Students with Career Indecision Using a Shortened Form of the Career Construction Interview

    Science.gov (United States)

    Rehfuss, Mark C.; Sickinger, Pamela H.

    2015-01-01

    A shortened form of the Career Construction Interview (CCI) was used to help high school students struggling with the career decision making process. The shortened instrument is described, as well as, its use with eleventh grade high school students who had low levels of career concern and career curiosity. Students who completed the exercise…

  13. Evaluation of canola oil oleogels with candelilla wax as an alternative to shortening in baked goods.

    Science.gov (United States)

    Jang, Areum; Bae, Woosung; Hwang, Hong-Sik; Lee, Hyeon Gyu; Lee, Suyong

    2015-11-15

    The oleogels of canola oil with candelilla wax were prepared and utilized as a shortening replacer to produce cookies with a high level of unsaturated fatty acids. The incorporation of candelilla wax (3% and 6% by weight) to canola oil produced the oleogels with solid-like properties. The firmness of the oleogels was lower than that of the shortening at room temperature. A more rapid change in the viscosity with temperature was observed with increasing levels of candelilla wax in the steady shear measurements. The replacement of shortening with oleogels in the cookie formulation reduced both viscoelastic parameters (G' and G") of the cookie doughs. The level of unsaturated fatty acids in the oleogel cookies was distinctly increased up to around 92%, compared to the shortening cookies (47.2%). The cookies with the oleogels showed desirable spreadable property and the replacement of shortening with the oleogels produced cookies with soft eating characteristics.

  14. Repeatability of corticospinal and spinal measures during lengthening and shortening contractions in the human tibialis anterior muscle.

    Directory of Open Access Journals (Sweden)

    Jamie Tallent

    Full Text Available UNLABELLED: Elements of the human central nervous system (CNS constantly oscillate. In addition, there are also methodological factors and changes in muscle mechanics during dynamic muscle contractions that threaten the stability and consistency of transcranial magnetic stimulation (TMS and perpherial nerve stimulation (PNS measures. PURPOSE: To determine the repeatability of TMS and PNS measures during lengthening and shortening muscle actions in the intact human tibialis anterior. METHODS: On three consecutive days, 20 males performed lengthening and shortening muscle actions at 15, 25, 50 and 80% of maximal voluntary contraction (MVC. The amplitude of the Motor Evoked Potentials (MEPs produced by TMS was measured at rest and during muscle contraction at 90° of ankle joint position. MEPs were normalised to Mmax determined with PNS. The corticospinal silent period was recorded at 80% MVC. Hoffman reflex (H-reflex at 10% isometric and 25% shortening and lengthening MVCs, and V-waves during MVCs were also evoked on each of the three days. RESULTS: With the exception of MEPs evoked at 80% shortening MVC, all TMS-derived measures showed good reliability (ICC = 0.81-0.94 from days 2 to 3. Confidence intervals (CI, 95% were lower between days 2 and 3 when compared to days 1 and 2. MEPs significantly increased at rest from days 1 to 2 (P = 0.016 and days 1 to 3 (P = 0.046. The H-reflex during dynamic muscle contraction was reliable across the three days (ICC = 0.76-0.84. V-waves (shortening, ICC = 0.77, lengthening ICC = 0.54 and the H-reflex at 10% isometric MVC (ICC = 0.66 was generally less reliable over the three days. CONCLUSION: Although it is well known that measures of the intact human CNS exhibit moment-to-moment fluctuations, careful experimental arrangements make it possible to obtain consistent and repeatable measurements of corticospinal and spinal excitability in the actively lengthening and shortening human

  15. Lipid Peroxidation-Mediated Telomere Shortening in Hydroxyl Radical-Induced Apoptosis in HeLa Cells

    Institute of Scientific and Technical Information of China (English)

    任建国; 陈晶; 戴尧仁

    2001-01-01

    Many anti-cancer drugs have been found to trigger apoptosis in tumor cells through the production of reactive oxygen species (ROS) including hydroxyl radicals (@ OH) regardless of chemical types. At the same time, telomerase is found to be associated with malignancy and reduced apoptosis. However, little is known about the linkage between ROS (such as @ OH) and telomerase/telomere. The focus of this investigation was to examine the possible pathway of the apoptosis induced by @ OH production via Fe2+ and H2O2. Results of the present study demonstrated that after exposure of HeLa cells to Fe2+-H2O2 system, an increase in lipid peroxidation and reduction of GSH was observed. These events proceeded and triggered apoptosis, resulting in DNA fragmentation. More interestingly, we did not observe any changes of telomerase activity. However, the telomere length in apoptotic cells shortened significantly. We also found that GSH rescued @ OH-induced HeLa cell death and prevened telomere shortening, and that 3,3'-diethyoxadicarbocyanine (DODCB), a telomerase inhibitor, increased susceptibility of HeLa cells to @ OH-induced apoptosis. Our results suggest that @ OH-induced telomere shortening is not through telomerase inhibition but possibly a direct effect of @ OH on telomeres themselves via lipid peroxidation.

  16. [Iliopsoas muscle syndrome. Functional disorders: shortening, spasm and weakness of a structurally unchanged muscle].

    Science.gov (United States)

    Grgić, Vjekoslav

    2009-01-01

    Functional (non-organic) disorders of the iliopsoas muscle (IPM), i.e. the shortening, spasm and weakness of the structurally unchanged IPM, can be manifested as abdominal and/or pelvic pain, pain in areas of the thoracolumbar (ThL) and lumbosacral (LS) spine, sacroiliac (SI) joint, hip, groin and anterior thigh on the side of the affected muscle as well as gait disturbances (iliopsoas muscle syndrome). By clinical examination of the IPM, including the transabdominal palpation, stretch and strength tests, pathological masses, shortening, painful spasm, weakness and tendon tenderness of that muscle can be diagnosed. The IPM is, like other postural muscles, inclined to shortening. The weakness of the IPM can be a consequence of the lesion of the lumbar plexus or femoral nerve that innervate the IPM, as well as a consequence of certain organic diseases of the IPM. Painful stimuli coming from somatic and visceral structures that are innervated from Th12-L4 nerve roots, from which the IPM segmental innervation also originates, can cause a reflex spasm of the IPM. A painful spasm of the IPM caused by disorders of the ThL and LS spine, SI and hip joint, can mimic diseases of the abdominal and pelvic organs. In the differential diagnosis of the IPM painful spasm, organic diseases of that muscle should be considered foremost (abscess, hematoma, tumor, metastase), as they can result in spasm, and the diseases of the abdominal and pelvic organs that can cause an IPM reflex spasm. The IPM functional disorders, which are not rare, are often overlooked during a clinical examination of a patient. Reasons for overlooking these disorders are: 1) a nonspecific and variable clinical picture presenting the IPM functional disorders, 2) the IPM functional disorders are a neglected source of pain, 3) the inaccessibility of the IPM for inspection, 4) the lack of knowledge of the IPM examination techniques and 5) the IPM functional disorders cannot be discovered by radiological

  17. Studies on Frying Quality of Virgin Coconut Oil and Shortening Blends

    Directory of Open Access Journals (Sweden)

    Muhammad Nor Omar

    2014-09-01

    Full Text Available The frying performances of palm solid shortening (PS and virgin coconut oil (VCO blends were evaluated. The fresh chickens were fried in a blended frying medium using an open fryer for 8 hours per day intermittently at 30 min interval for five consecutive days. Three types of oils were used; i.e. palm shortening without blending (PS, a blend of VCO: PS (10:90, and a blend of VCO: PS (20:80. The frying oils were collected periodically and their fatty acid composition (FAC, free fatty acid (FFA content, total polar compound (TPC and total polymer material (TPM were determined. In addition, the fried chicken was subjected to the analyses of oil absorption and sensory evaluation. The results showed that the frying performance of the VCO:PS (20:80 blend was better than VCO:PS (10:90 blend and PS in terms of TPM and TPC contents. The VCO:PS (20:80 gave TPC and TPM values of 17.4 and 1.40% respectively, the blend of VCO:PS (10:90 gave values of TPC and TPM of 18. 7 and 2.20% respectively, whilst PS with TPC of and TPM of 20.5 and 3.02 % respectively. Meanwhile, the oil absorption patterns showed that the fried chicken in VCO: PS (10:90 blend absorbed less oil compared to chickens fried in PS and VCO:PS (20:80 blend. The oil uptake in chickens fried in VCO:PS (10:90 blend was 1.75%, while in PS and VCO:PS (20:80 blend were 2.82 and 2.57 % respectively. In term of sensory evaluation, the addition of virgin coconut oil (VCO to palm based solid shortening (PS showed significant differences (p < 0.05 on crispiness, taste and overall scores on the 1st , 3rd and 5th day of frying. Although there were no significant difference on oiliness on the 1st and 3rd day, there was a significant difference on the 5th day of frying. In conclusion, the addition of a small proportion of VCO in PS would improve the frying performance of oil as well as sensory scores of the fried products.

  18. Ankle morphology amplifies calcaneus movement relative to triceps surae muscle shortening.

    Science.gov (United States)

    Csapo, R; Hodgson, J; Kinugasa, R; Edgerton, V R; Sinha, S

    2013-08-15

    The present study investigated the mechanical role of the dorsoventral curvature of the Achilles tendon in the conversion of the shortening of the plantarflexor muscles into ankle joint rotation. Dynamic, sagittal-plane magnetic resonance spin-tagged images of the ankle joint were acquired in six healthy subjects during both passive and active plantarflexion movements driven by a magnetic resonance compatible servomotor-controlled foot-pedal device. Several points on these images were tracked to determine the 1) path and deformation of the Achilles tendon, 2) ankle's center of rotation, and 3) tendon moment arms. The degree of mechanical amplification of joint movement was calculated as the ratio of the displacements of the calcaneus and myotendinous junction. In plantarflexion, significant deflection of the Achilles tendon was evident in both the passive (165.7 ± 7.4°; 180° representing a straight tendon) and active trials (166.9 ± 8.8°). This bend in the dorsoventral direction acts to move the Achilles tendon closer to the ankle's center of rotation, resulting in an ∼5% reduction of moment arm length. Over the entire range of movement, the overall displacement of the calcaneus exceeded the displacement of the myotendinous junction by ∼37%, with the mechanical gains being smaller in dorsi- and larger in plantarflexed joint positions. This is the first study to assess noninvasively and in vivo using MRI the curvature of the Achilles tendon during both passive and active plantarflexion movements. The dorsoventral tendon curvature amplifies the shortening of the plantarflexor muscles, resulting in a greater displacement of the tendon's insertion into the calcaneus compared with its origin.

  19. Shortening Infusion Time for High-Dose Methotrexate Alters Antileukemic Effects: A Randomized Prospective Clinical Trial

    Science.gov (United States)

    Mikkelsen, Torben S.; Sparreboom, Alex; Cheng, Cheng; Zhou, Yinmei; Boyett, James M.; Raimondi, Susana C.; Panetta, John C.; Bowman, W. Paul; Sandlund, John T.; Pui, Ching-Hon; Relling, Mary V.; Evans, William E.

    2011-01-01

    Purpose To determine whether shortening the infusion duration of high-dose methotrexate (HDMTX; 1 g/m2) affects the in vivo accumulation of active methotrexate polyglutamates (MTXPG1-7) in leukemia cells and whether this differs among major acute lymphoblastic leukemia (ALL) subtypes. Methods From June 2000 through October 2007, 356 children with ALL were randomly assigned to receive initial single-agent treatment with HDMTX (1 g/m2) as either a 24-hour infusion or a 4-hour infusion at two pediatric hospitals in the United States. The primary outcome measures were the accumulation of MTXPG1-7 in leukemia cells and the antileukemic effects (eg, inhibition of de novo purine synthesis in bone marrow ALL cells, and decrease in circulating ALL cells). Results The 24-hour infusion resulted in significantly higher amounts of MTXPG1-7 in bone marrow leukemia cells (median: 1,695 v 1,150 pmol/109 cells, P = .0059), and better antileukemic effects. The 24-hour infusion had the greatest effect on MTXPG1-7 accumulation in hyperdiploid ALL (median: 3,919 v 2,417 pmol/109 cells, P = .0038); T-cell ALL exhibited smaller differences in MTXPG1-7 but greater antileukemic effects with the longer infusion (median decrease in leukemia cells: 88.4% v 51.8%, P = .0075). In contrast, infusion duration had no significant impact on MTXPG1-7 accumulation or antileukemic effects in ALL with the t(12;21)/(ETV6-RUNX1) chromosomal translocation. Conclusion Shortening the infusion time of HDMTX reduces accumulation of active methotrexate in leukemia cells and decreases antileukemic effects, with differing consequences among major ALL subtypes. PMID:21444869

  20. Feline chronic kidney disease is associated with shortened telomeres and increased cellular senescence.

    Science.gov (United States)

    Quimby, Jessica M; Maranon, David G; Battaglia, Christine L R; McLeland, Shannon M; Brock, William T; Bailey, Susan M

    2013-08-01

    Telomeres are protective structures at the ends of chromosomes that have important implications for aging. To address the question of whether telomeres contribute to feline chronic kidney disease (CKD), we evaluated kidney, liver, and skin samples from 12 cats with naturally occurring CKD, 12 young normal cats, and 6 old normal cats. Telomere length was assessed using standard telomere fluorescent in situ hybridization (TEL-FISH) combined with immunohistochemistry (TELI-FISH) to identify proximal (PTEC) and distal tubular epithelial cells (DTEC), whereas senescence-associated β-galactosidase (SABG) staining was used to evaluate senescence. Results revealed statistically significant decreases in the average telomere fluorescence intensity (TFI) of PTEC in CKD cats compared with young and geriatric normal cats, and in the DTEC of CKD cats compared with young normal cats. When histograms of individual TFI were compared, statistically significant decreases in the PTEC and DTEC of CKD cats were observed compared with young and geriatric normal cats. Concomitantly, a statistically significant increase in SABG staining was seen in CKD kidney samples compared with young normal cats. CKD cats tended to have increased SABG staining in the kidney compared with normal geriatric cats, but this did not reach statistical significance. No significant telomere shortening in liver or skin from any group was observed. Real-time quantitative telomeric repeat amplification protocol assessment of renal telomerase activity revealed comparable low levels of telomerase activity in all groups. Our results suggest that shortened telomeres and increased senescence in the kidneys of CKD cats may represent novel targets for interventional therapy.

  1. Unloaded shortening velocity of voluntarily and electrically activated human dorsiflexor muscles in vivo.

    Directory of Open Access Journals (Sweden)

    Kazushige Sasaki

    Full Text Available We have previously shown that unloaded shortening velocity (V(0 of human plantar flexors can be determined in vivo, by applying the "slack test" to submaximal voluntary contractions (J Physiol 567:1047-1056, 2005. In the present study, to investigate the effect of motor unit recruitment pattern on V(0 of human muscle, we modified the slack test and applied this method to both voluntary and electrically elicited contractions of dorsiflexors. A series of quick releases (i.e., rapid ankle joint rotation driven by an electrical dynamometer was applied to voluntarily activated dorsiflexor muscles at three different contraction intensities (15, 50, and 85% of maximal voluntary contraction; MVC. The quick-release trials were also performed on electrically activated dorsiflexor muscles, in which three stimulus conditions were used: submaximal (equal to 15%MVC 50-Hz stimulation, supramaximal 50-Hz stimulation, and supramaximal 20-Hz stimulation. Modification of the slack test in vivo resulted in good reproducibility of V(0, with an intraclass correlation coefficient of 0.87 (95% confidence interval: 0.68-0.95. Regression analysis showed that V(0 of voluntarily activated dorsiflexor muscles significantly increased with increasing contraction intensity (R(2 = 0.52, P<0.001. By contrast, V(0 of electrically activated dorsiflexor muscles remained unchanged (R(2<0.001, P = 0.98 among three different stimulus conditions showing a large variation of tetanic torque. These results suggest that the recruitment pattern of motor units, which is quite different between voluntary and electrically elicited contractions, plays an important role in determining shortening velocity of human skeletal muscle in vivo.

  2. Specific modulation of corticospinal and spinal excitabilities during maximal voluntary isometric, shortening and lengthening contractions in synergist muscles.

    Science.gov (United States)

    Duclay, Julien; Pasquet, Benjamin; Martin, Alain; Duchateau, Jacques

    2011-06-01

    This study was designed to investigate the cortical and spinal mechanisms involved in the modulations of neural activation during lengthening compared with isometric and shortening maximal voluntary contractions (MVCs). Two muscles susceptible to different neural adjustments at the spinal level, the soleus (SOL) and medial gastrocnemius (MG), were compared. Twelve healthy males participated in at least two experimental sessions designed to assess corticospinal and spinal excitabilities. We compared the modulation of motor evoked potentials (MEPs) in response to transcranial magnetic stimulation and Hoffmann reflexes (H-reflexes) during isometric and anisometric MVCs. The H-reflex and MEP responses, recorded during lengthening and shortening MVCs, were compared with those obtained during isometric MVCs. The results indicate that the maximal amplitude of both MEP and H-reflex in the SOL were smaller (P shortening MVCs but similar (P > 0.05) in MG for all three muscle contraction types. The silent period that follows maximal MEPs was reduced (P muscle. Collectively, the current results indicate that the relative contribution of both cortical and spinal mechanisms to the modulation of neural activation differs during lengthening MVCs and between two synergist muscles. The comparison of SOL and MG responses further suggests that the specific modulation of the corticospinal excitability during lengthening MVCs depends mainly on pre- and postsynaptic inhibitory mechanisms acting at the spinal level.

  3. Calculation of muscle maximal shortening velocity by extrapolation of the force-velocity relationship: afterloaded versus isotonic release contractions.

    Science.gov (United States)

    Bullimore, Sharon R; Saunders, Travis J; Herzog, Walter; MacIntosh, Brian R

    2010-10-01

    The maximal shortening velocity of a muscle (V(max)) provides a link between its macroscopic properties and the underlying biochemical reactions and is altered in some diseases. Two methods that are widely used for determining V(max) are afterloaded and isotonic release contractions. To determine whether these two methods give equivalent results, we calculated V(max) in 9 intact single fibres from the lumbrical muscles of the frog Xenopus laevis (9.5-15.5 °C, stimulation frequency 35-70 Hz). The data were modelled using a 3-state cross-bridge model in which the states were inactive, detached, and attached. Afterloaded contractions gave lower predictions of Vmax than did isotonic release contractions in all 9 fibres (3.20 ± 0.84 versus 4.11 ± 1.08 lengths per second, respectively; means ± SD, p = 0.001) and underestimated unloaded shortening velocity measured with the slack test by an average of 29% (p = 0.001, n = 6). Excellent model predictions could be obtained by assuming that activation is inhibited by shortening. We conclude that under the experimental conditions used in this study, afterloaded and isotonic release contractions do not give equivalent results. When a change in the V(max) measured with afterloaded contractions is observed in diseased muscle, it is important to consider that this may reflect differences in either activation kinetics or cross-bridge cycling rates.

  4. Impaired neuromuscular function during isometric, shortening, and lengthening contractions after exercise-induced damage to elbow flexor muscles.

    Science.gov (United States)

    Turner, Tanya S; Tucker, Kylie J; Rogasch, Nigel C; Semmler, John G

    2008-08-01

    The purpose of this study was to examine the effect of exercise-induced damage of the elbow flexor muscles on steady motor performance during isometric, shortening, and lengthening contractions. Ten healthy individuals (age 22+/-4 yr) performed four tasks with the elbow flexor muscles: a maximum voluntary contraction, a one repetition maximum (1 RM), an isometric task at three joint angles (short, intermediate, and long muscle lengths), and a constant-load task during slow (approximately 7 degrees/s) shortening and lengthening contractions. Task performance was quantified as the fluctuations in wrist acceleration (steadiness), and electromyography was obtained from the biceps and triceps brachii muscles at loads of 10, 20, and 40% of 1 RM. Tasks were performed before, immediately after, and 24 h after eccentric exercise that resulted in indicators of muscle damage. Maximum voluntary contraction force and 1-RM load declined by approximately 45% immediately after exercise and remained lower at 24 h ( approximately 30% decrease). Eccentric exercise resulted in reduced steadiness and increased biceps and triceps brachii electromyography for all tasks. For the isometric task, steadiness was impaired at the short compared with the long muscle length immediately after exercise (Pshortening compared with the lengthening contractions after exercise (P=0.01), and steadiness remained impaired for shortening contractions 24 h later (P=0.01). These findings suggest that there are profound effects for the performance of these types of fine motor tasks when recovering from a bout of eccentric exercise.

  5. Specific modulation of spinal and cortical excitabilities during lengthening and shortening submaximal and maximal contractions in plantar flexor muscles.

    Science.gov (United States)

    Duclay, Julien; Pasquet, Benjamin; Martin, Alain; Duchateau, Jacques

    2014-12-15

    This study investigated the influence of the torque produced by plantar flexor muscles on cortical and spinal excitability during lengthening and shortening voluntary contractions. To that purpose, modulations of motor-evoked potential (MEP) and Hoffmann (H) reflex were compared in the soleus (SOL) and medial gastrocnemius (MG) during anisometric submaximal and maximal voluntary contraction (MVC) of the plantar flexor muscles. For the submaximal shortening and lengthening contractions, the target torque was set at 50% of their respective MVC force. The results indicate that the amplitudes of both MEP and H-reflex responses, normalized to the maximal M wave, were significantly (P 0.05) was observed for MG. In addition, the silent period in the ongoing electromyogram (EMG) activity following the MEP was significantly (P 0.05) between contraction intensities and muscles. Together, these results indicate that cortical and spinal mechanisms involved in the modulation of muscle activation during shortening and lengthening contractions differ between synergistic muscles according to the torque produced. Data further document previous studies reporting that the specific modulation of muscle activation during lengthening contraction is not torque dependent.

  6. HIV Infection Is Associated with Shortened Telomere Length in Ugandans with Suspected Tuberculosis

    Science.gov (United States)

    Auld, Elizabeth; Lin, Jue; Chang, Emily; Byanyima, Patrick; Ayakaka, Irene; Musisi, Emmanuel; Worodria, William; Davis, J. Lucian; Segal, Mark; Blackburn, Elizabeth; Huang, Laurence

    2016-01-01

    Introduction HIV infection is a risk factor for opportunistic pneumonias such as tuberculosis (TB) and for age-associated health complications. Short telomeres, markers of biological aging, are also associated with an increased risk of age-associated diseases and mortality. Our goals were to use a single cohort of HIV-infected and HIV-uninfected individuals hospitalized with pneumonia to assess whether shortened telomere length was associated with HIV infection, TB diagnosis, and 2-month mortality. Methods This was a sub-study of the IHOP Study, a prospective observational study. Participants consisted of 184 adults admitted to Mulago Hospital in Kampala, Uganda who underwent evaluation for suspected TB and were followed for 2 months. Standardized questionnaires were administered to collect demographic and clinical data. PBMCs were isolated and analyzed using quantitative PCR to determine telomere length. The association between HIV infection, demographic and clinical characteristics, and telomere length was assessed, as were the associations between telomere length, TB diagnosis and 2-month mortality. Variables with a P≤0.2 in bivariate analysis were included in multivariate models. Results No significant demographic or clinical differences were observed between the HIV-infected and HIV-uninfected subjects. Older age (Ptelomere length in bivariate analysis. In multivariate analysis adjusting for these five variables, HIV-positive participants had significantly shorter telomeres than HIV-negative participants (β = -0.0621, 95% CI -0.113 to -0.011, P = 0.02). Shortened telomeres were not associated with TB or short-term mortality. Conclusions The association between HIV infection and shorter telomeres suggests that HIV may play a role in cellular senescence and biological aging and that shorter telomeres may be involved in age-associated health complications seen in this population. The findings indicate a need to further research the impact of HIV on aging. PMID

  7. New reconstruction algorithm allows shortened acquisition time for myocardial perfusion SPECT

    Energy Technology Data Exchange (ETDEWEB)

    Valenta, Ines; Treyer, Valerie; Husmann, Lars; Gaemperli, Oliver; Schindler, Michael J.; Herzog, Bernhard A.; Veit-Heibach, Patrick; Pazhenkottil, Aju P.; Kaufmann, Philipp A. [University Hospital Zurich, Cardiac Imaging, Zurich (Switzerland); University of Zurich, Zurich Center for Integrative Human Physiology, Zurich (Switzerland); Buechel, Ronny R.; Nkoulou, Rene [University Hospital Zurich, Cardiac Imaging, Zurich (Switzerland)

    2010-04-15

    Shortening scan time and/or reducing radiation dose at maintained image quality are the main issues of the current research in radionuclide myocardial perfusion imaging (MPI). We aimed to validate a new iterative reconstruction (IR) algorithm for SPECT MPI allowing shortened acquisition time (HALF time) while maintaining image quality vs. standard full time acquisition (FULL time). In this study, 50 patients, referred for evaluation of known or suspected coronary artery disease by SPECT MPI using 99mTc-Tetrofosmin, underwent 1-day adenosine stress 300 MBq/rest 900 MBq protocol with standard (stress 15 min/rest 15 min FULL time) immediately followed by short emission scan (stress 9 min/rest 7 min HALF time) on a Ventri SPECT camera (GE Healthcare). FULL time scans were processed with IR, short scans were additionally processed with a recently developed software algorithm for HALF time emission scans. All reconstructions were subsequently analyzed using commercially available software (QPS/QGS, Cedars Medical Sinai) with/without X-ray based attenuation correction (AC). Uptake values (percent of maximum) were compared by regression and Bland-Altman (BA) analysis in a 20-segment model. HALF scans yielded a 96% readout and 100% clinical diagnosis concordance compared to FULL. Correlation for uptake in each segment (n = 1,000) was r = 0.87at stress (p < 0.001) and r = 0.89 at rest (p < 0.001) with respective BA limits of agreement of -11% to 10% and -12% to 11%. After AC similar correlation (r = 0.82, rest; r = 0.80, stress, both p < 0.001) and BA limits were found (-12% to 10%; -13% to 12%). With the new IR algorithm, SPECT MPI can be acquired at half of the scan time without compromising image quality, resulting in an excellent agreement with FULL time scans regarding to uptake and clinical conclusion. (orig.)

  8. Maximum shortening velocity of lymphatic muscle approaches that of striated muscle.

    Science.gov (United States)

    Zhang, Rongzhen; Taucer, Anne I; Gashev, Anatoliy A; Muthuchamy, Mariappan; Zawieja, David C; Davis, Michael J

    2013-11-15

    Lymphatic muscle (LM) is widely considered to be a type of vascular smooth muscle, even though LM cells uniquely express contractile proteins from both smooth muscle and cardiac muscle. We tested the hypothesis that LM exhibits an unloaded maximum shortening velocity (Vmax) intermediate between that of smooth muscle and cardiac muscle. Single lymphatic vessels were dissected from the rat mesentery, mounted in a servo-controlled wire myograph, and subjected to isotonic quick release protocols during spontaneous or agonist-evoked contractions. After maximal activation, isotonic quick releases were performed at both the peak and plateau phases of contraction. Vmax was 0.48 ± 0.04 lengths (L)/s at the peak: 2.3 times higher than that of mesenteric arteries and 11.4 times higher than mesenteric veins. In cannulated, pressurized lymphatic vessels, shortening velocity was determined from the maximal rate of constriction [rate of change in internal diameter (-dD/dt)] during spontaneous contractions at optimal preload and minimal afterload; peak -dD/dt exceeded that obtained during any of the isotonic quick release protocols (2.14 ± 0.30 L/s). Peak -dD/dt declined with pressure elevation or activation using substance P. Thus, isotonic methods yielded Vmax values for LM in the mid to high end (0.48 L/s) of those the recorded for phasic smooth muscle (0.05-0.5 L/s), whereas isobaric measurements yielded values (>2.0 L/s) that overlapped the midrange of values for cardiac muscle (0.6-3.3 L/s). Our results challenge the dogma that LM is classical vascular smooth muscle, and its unusually high Vmax is consistent with the expression of cardiac muscle contractile proteins in the lymphatic vessel wall.

  9. Analysis of combination drug therapy to develop regimens with shortened duration of treatment for tuberculosis.

    Science.gov (United States)

    Drusano, George L; Neely, Michael; Van Guilder, Michael; Schumitzky, Alan; Brown, David; Fikes, Steven; Peloquin, Charles; Louie, Arnold

    2014-01-01

    Tuberculosis remains a worldwide problem, particularly with the advent of multi-drug resistance. Shortening therapy duration for Mycobacterium tuberculosis is a major goal, requiring generation of optimal kill rate and resistance-suppression. Combination therapy is required to attain the goal of shorter therapy. Our objective was to identify a method for identifying optimal combination chemotherapy. We developed a mathematical model for attaining this end. This is accomplished by identifying drug effect interaction (synergy, additivity, antagonism) for susceptible organisms and subpopulations resistant to each drug in the combination. We studied the combination of linezolid plus rifampin in our hollow fiber infection model. We generated a fully parametric drug effect interaction mathematical model. The results were subjected to Monte Carlo simulation to extend the findings to a population of patients by accounting for between-patient variability in drug pharmacokinetics. All monotherapy allowed emergence of resistance over the first two weeks of the experiment. In combination, the interaction was additive for each population (susceptible and resistant). For a 600 mg/600 mg daily regimen of linezolid plus rifampin, we demonstrated that >50% of simulated subjects had eradicated the susceptible population by day 27 with the remaining organisms resistant to one or the other drug. Only 4% of patients had complete organism eradication by experiment end. These data strongly suggest that in order to achieve the goal of shortening therapy, the original regimen may need to be changed at one month to a regimen of two completely new agents with resistance mechanisms independent of the initial regimen. This hypothesis which arose from the analysis is immediately testable in a clinical trial.

  10. Shortening of the 3' untranslated region: an important mechanism leading to overexpression of HMGA2 in serous ovarian cancer

    Institute of Scientific and Technical Information of China (English)

    He Xiangjun; Yang Jing; Zhang Qi; Cui Heng; Zhang Yujun

    2014-01-01

    Background Oncofetal protein high-mobility-group AT-hook protein 2 (HMGA2) is reactivated in serous ovarian cancer (SOC) and its overexpression correlates with poor prognosis.To explore the mechanism,we investigated whether HMGA2 could avoid microRNA regulation due to gene truncation or 3' UTR shortening by alternative polyadenylation.Methods Real-time reverse transcription polymerase chain reaction (RT-PCR) was used to evaluate the abundance of different regions of HMGA2 mRNA in 46 SOC samples.Rapid amplification of cDNA 3' ends (3' RACE) and Southern blotting were used to confirm the shortening of 3' untranslated region (UTR).5' RACE and Southern blotting were used to prove the mRNA decay.Results No significant difference in the ratio of the stable coding region to the fragile region was observed between SOC and control normal fallopian tubes,indicating that the HMGA2 gene is not truncated in SOC.Varying degrees of 3' UTR shortening in SOC samples were observed by comparing the abundance of the proximal region and distal region of the HMGA2 3' UTR.The ratio of the proximal to the distal region of the 3' UTR correlated significantly with expression of the HMGA2 coding region in SOC (r=0.579,P <0.01).Moreover,although the abundance of the HMGA2 coding region varied,all samples,including the very low expressed samples,exhibit relatively high levels of the proximal 3' UTR region,suggesting a dynamic decay of HMGA2 mRNA from the 5' end.The shortening of 3' UTR and the decay from the 5' end were confirmed by 3' RACE,5' RACE and subsequent Southern blotting.Conclusion Heterogeneous 3' UTR lengths render HMGA2 susceptible to different levels of negative regulation by microRNAs,which represents an important mechanism of HMGA2 reactivation in SOC.

  11. Effect of Pre-gelatinized Wheat Starch on Physical and Rheological Properties of Shortened Cake Batter and Cake Texture

    Directory of Open Access Journals (Sweden)

    F. Ebrahimi

    2016-10-01

    Full Text Available The focus of this study was the effect of 1.5%, 3% and 4.5% pre-gelatinized wheat starch (based on the total weight of cake batter on improving the qualitative properties of shortened cake batter. Specific volume and viscosity of the shortened cake batter were measured for controls, 1.5%, 3% and 4.5% gelatinized starch; some important properties such as texture and sensory evaluation were examined. By increasing pre-gelatinized wheat starch used in the batter, a significant difference was observed in the rheological properties of the batter. Cake batter properties were found improved compared to the control samples. The sample with 3% pre-gelatinized starch had a lower viscosity than other treatments. The treatment with 4.5% pre-gelatinized starch had the lowest specific volume compared to other treatments. The overall results showed that the shortened cake with 3% pre-gelatinized starch was the best treatment in terms of texture and sensory evaluation factors.

  12. S-ROM modular arthroplasty combined with transverse subtrochanteric shortening for Crowe type IV congenital dislocation of hip

    Institute of Scientific and Technical Information of China (English)

    ZHONG Cheng; CAI Xun-zi; YAN Shi-gui; HE Rong-xin

    2011-01-01

    Background This work was carried out to evaluate the clinical efficacy and the complications of S-ROM modular hip arthroplasty combined with transverse subtrochanteric shortening for Crowe type IV congenital dislocation of the hip (CDH).Methods A totoal of 28 consecutive patients with Crowe type IV CDH received treatment using this surgical technique from June 2003 to June 2010.The follow-up was conducted at 3 days,1,6,and 12 months after the operation and later annually at the outpatient of our hospital.Sequential pelvic plain film and normotopia film of the affected hip joint were taken.The limp and the Trendelenburg sign were also assessed,the ischiadic nerve injury was also evaluated by electromyogram,and Harris hip scores were recorded.Results After operation,both the alignment and the position of the transverse osteotomies were good.None of the patients had presented complications of joint infection,prosthesis loosening,joint dislocation,or nerve injury.Conclusions S-ROM modular hip arthroplasty combined with transverse subtrochanteric shortening was a satisfactory and safe technique for the Crowe type IV congenital hip dislocation within a mean follow up of 53 months.Transverse subtrochanteric shortening could effectively prevent the distraction injury of sciatic nerve.

  13. Cementless arthroplasty with a distal femoral shortening for the treatment of Crowe type IV developmental hip dysplasia.

    Science.gov (United States)

    Guo, Chang-Yong; Liang, Bo-Wei; Sha, Mo; Kang, Liang-Qi; Wang, Jiang-Ze; Ding, Zhen-Qi

    2015-01-01

    Severe developmental dysplasia of the hip is a surgical challenge. The purpose of this study is to describe the cementless arthroplasty with a distal femoral shortening osteotomy for Crowe type IV developmental hip dysplasia and to report the results of this technique. 12 patients (2 male and 10 female) of Crowe type IV developmental hip dysplasia operated between January 2005 and December 2010 were included in the study. All had undergone cementless arthroplasty with a distal femoral shortening osteotomy. Acetabular cup was placed at the level of the anatomical position in all the hips. The clinical outcomes were assessed and radiographs were reviewed to evaluate treatment effects. The mean followup for the 12 hips was 52 months (range 36-82 months). The mean Harris hip score improved from 41 points (range 28-54) preoperatively to 85 points (range 79-92) at the final followup. The mean length of bone removed was 30 mm (range 25-40 mm). All the osteotomies healed in a mean time of 13 weeks (range 10-16 weeks). There were no neurovascular injuries, pulmonary embolism or no infections. Our study suggests that cementless arthroplasty with a distal femoral shortening is a safe and effective procedure for severe developmental dysplasia of the hip.

  14. Telomere shortening in diaphragm and tibialis anterior muscles of aged mdx mice.

    Science.gov (United States)

    Lund, Troy C; Grange, Robert W; Lowe, Dawn A

    2007-09-01

    The progression of Duchenne muscular dystrophy (DMD) is, in part, due to satellite cell senescence driven by high replicative pressure as these muscle stem cells repeatedly divide and fuse to damaged muscle fibers. We hypothesize that telomere shortening in satellite cells underlies their senescence. To test this hypothesis, we evaluated the diaphragm and a leg muscle from dystrophic mice of various ages for telomere dynamics. We found 30% telomere shortening in tibialis anterior muscles from 600-day-old mdx mice relative to age-matched wildtype mice. We also found a more severe shortening of telomere length in diaphragm muscles of old mdx mice. In those muscles, telomeres were shortened by approximately 15% and 40% in 100- and 600-day-old mdx mice, respectively. These findings indicate that satellite cells undergo telomere erosion, which may contribute to the inability of these cells to perpetually repair DMD muscle.

  15. Comparison of Surgical Efficacy of Levator Muscle Short-ening and Modified Levator Aponeurosis Tucking in Treat-ing Minimal and Moderate Congenital Blepharoptosis

    Institute of Scientific and Technical Information of China (English)

    Chong Wang; Yanli Wang

    2015-01-01

    Purpose:.To evaluate the surgical effect of levator muscle shortening and levator aponeurosis tucking in treating minimal and moderate congenital blepharoptosis. Methods:.Clinical data of 28 patients (40 eyes) diagnosed with mide and moderate congenital blepharoptosis at our institution were retrospectively analyzed. Postoperative efficacy was eval-uated and statistically compared between these two techniques. Results:.During 14 months follow-up,.16 eyes with ptosis un-dergoing levator muscle shortening were treated,.3 with un-dercorrection of ptosis and 1 with overcorrection of ptosis.In patients receiving levator aponeurosis tucking,.16 eyes were cured and 4 with undercorrection of ptosis. Conclusion:.Both levator muscle shortening and levator aponeurosis tucking are safe and efficacious for correcting minimal and moderate congenital blepharoptosis.

  16. Attempts to Shorten the Time of Lactic Fermentation by Ultrasonic Irradiation

    Science.gov (United States)

    Masuzawa, Nobuyoshi; Ohdaira, Etsuzo

    2002-05-01

    In recent years, applications of ultrasound to food processing have been of interest. Fermentation is a typical example of food processing which has been used, since ancient times, on milk and is utilized for processing various dairy products, e.g., yoghurt. In this study, ultrasonic irradiation to shorten the time of fermentation in yoghurt production is attempted. It is proven that shortening the fermentation time is possible by employing ultrasonic irradiation.

  17. Compression and Combining Based on Channel Shortening and Rank Reduction Technique for Cooperative Wireless Sensor Networks

    KAUST Repository

    Ahmed, Qasim Zeeshan

    2013-12-18

    This paper investigates and compares the performance of wireless sensor networks where sensors operate on the principles of cooperative communications. We consider a scenario where the source transmits signals to the destination with the help of L sensors. As the destination has the capacity of processing only U out of these L signals, the strongest U signals are selected while the remaining (L?U) signals are suppressed. A preprocessing block similar to channel-shortening is proposed in this contribution. However, this preprocessing block employs a rank-reduction technique instead of channel-shortening. By employing this preprocessing, we are able to decrease the computational complexity of the system without affecting the bit error rate (BER) performance. From our simulations, it can be shown that these schemes outperform the channel-shortening schemes in terms of computational complexity. In addition, the proposed schemes have a superior BER performance as compared to channel-shortening schemes when sensors employ fixed gain amplification. However, for sensors which employ variable gain amplification, a tradeoff exists in terms of BER performance between the channel-shortening and these schemes. These schemes outperform channel-shortening scheme for lower signal-to-noise ratio.

  18. Could an increase in airway smooth muscle shortening velocity cause airway hyperresponsiveness?

    Science.gov (United States)

    Bullimore, Sharon R; Siddiqui, Sana; Donovan, Graham M; Martin, James G; Sneyd, James; Bates, Jason H T; Lauzon, Anne-Marie

    2011-01-01

    Airway hyperresponsiveness (AHR) is a characteristic feature of asthma. It has been proposed that an increase in the shortening velocity of airway smooth muscle (ASM) could contribute to AHR. To address this possibility, we tested whether an increase in the isotonic shortening velocity of ASM is associated with an increase in the rate and total amount of shortening when ASM is subjected to an oscillating load, as occurs during breathing. Experiments were performed in vitro using 27 rat tracheal ASM strips supramaximally stimulated with methacholine. Isotonic velocity at 20% isometric force (Fiso) was measured, and then the load on the muscle was varied sinusoidally (0.33 ± 0.25 Fiso, 1.2 Hz) for 20 min, while muscle length was measured. A large amplitude oscillation was applied every 4 min to simulate a deep breath. We found that: 1) ASM strips with a higher isotonic velocity shortened more quickly during the force oscillations, both initially (P shortening during the force oscillation protocol (P shortening with increased isotonic velocity could be explained by a change in either the cycling rate of phosphorylated cross bridges or the rate of myosin light chain phosphorylation. We conclude that, if asthma involves an increase in ASM velocity, this could be an important factor in the associated AHR.

  19. Chronic circadian disturbance by a shortened light-dark cycle increases mortality.

    Science.gov (United States)

    Park, Noheon; Cheon, Solmi; Son, Gi Hoon; Cho, Sehyung; Kim, Kyungjin

    2012-06-01

    Chronic circadian disturbance, a condition of desynchronization between endogenous clock and environmental light-dark (LD) cycle, is known to cause adverse physiological changes including mortality. However, it is yet unclear whether these consequences result from disturbance of endogenous clock or condition of the LD cycle per se. To address this issue, we imposed 3 different periods of LD cycle (T) on wild type and functional clock-defective (Per1(-/-)Per2(-/-)) mice. We found that the disturbed rhythms of locomotor activity and body temperature resulted from interaction of endogenous clock and T cycle and the chronic state of the disturbance suppressed the endogenous circadian rhythm. Interestingly, the endogenous clock and the T cycles affected body weight and food intake independently, while their interaction affected the life span resulting increased mortality of wild type mice in a shortened T cycle. These results strongly indicate the presence of both separate and combined effects of the endogenous clock and T cycle on different physiological variables implying that shift work scheduling can be an important influence on health parameters.

  20. Home Visits: Shortening the Path between Home and School.

    Science.gov (United States)

    Acosta, Dolores; Keith, Joe; Patin, Debra

    1997-01-01

    Describes the Home Visit Initiative at Socorro Middle School in Texas. Discusses the purpose of home visits; selection of families for teacher home visits; the visiting team; parents', students', and teachers' reactions to visits, and the results of an evaluation of the home visits, especially the impact on parent volunteerism and schooling,…

  1. Diaphragm curvature modulates the relationship between muscle shortening and volume displacement.

    Science.gov (United States)

    Greybeck, Brad J; Wettergreen, Matthew; Hubmayr, Rolf D; Boriek, Aladin M

    2011-07-01

    During physiological spontaneous breathing maneuvers, the diaphragm displaces volume while maintaining curvature. However, with maximal diaphragm activation, curvature decreases sharply. We tested the hypotheses that the relationship between diaphragm muscle shortening and volume displacement (VD) is nonlinear and that curvature is a determinant of such a relationship. Radiopaque markers were surgically placed on three neighboring muscle fibers in the midcostal region of the diaphragm in six dogs. The three-dimensional locations were determined using biplanar fluoroscopy and diaphragm VD, curvature, and muscle shortening were computed in the prone and supine postures during spontaneous breathing (SB), spontaneous inspiration efforts after airway occlusion at lung volumes ranging from functional residual capacity (FRC) to total lung capacity, and during bilateral maximal phrenic nerve stimulation at those same lung volumes. In supine dogs, diaphragm VD was approximately two- to three-fold greater during maximal phrenic nerve stimulation than during SB. The contribution of muscle shortening to VD nonlinearly increases with level of diaphragm activation independent of posture. During submaximal diaphragm activation, the contribution is essentially linear due to constancy of diaphragm curvature in both the prone and supine posture. However, the sudden loss of curvature during maximal bilateral phrenic nerve stimulation at muscle shortening values greater than 40% (ΔL/L(FRC)) causes a nonlinear increase in the contribution of muscle shortening to diaphragm VD, which is concomitant with a nonlinear change in diaphragm curvature. We conclude that the nonlinear relationship between diaphragm muscle shortening and its VD is, in part, due to a loss of its curvature at extreme muscle shortening.

  2. Depressant effect of active shortening in the anterior byssus retractor muscle of Mytilus edulis.

    Science.gov (United States)

    Ekelund, M C

    1983-03-01

    The effect of shortening during activity, previously characterized in vertebrate striated muscle, was investigated in the anterior byssus retractor muscle (ABRM) of the mollusc Mytilus edulis. This muscle is considered to have an essentially myosin-linked Ca2+-regulatory system. Release steps of different amplitude were performed during isometric phasic contraction, and force redevelopment was recorded at a muscle length L1, defined as 90% of the muscle length at which a slight resting tension, approximately 1 mN, appeared in the presence of 2.5 X 10(-5) M 5-HT. Active shortening caused a graded depression of the contractile force without affecting the total duration of the mechanical response. Peak redeveloped force after muscle shortening of 0.06 L1 and 0.18 L1 was reduced by approximately 1.5% and 7.0%, respectively, of the isometric tension value at L1. The shortening effect was fully reversible, and had a lifetime of approximately 8 to 9 s. The depressant effect of active shortening was augmented at a reduced degree of activation of the muscle. The presence of caffeine and dantrolene and altered tonicity of the extracellular medium (0.9 T-1.2 T) did not significantly affect the shortening induced depression obtained at maximum phasic activation of the preparation. The nature of the shortening effect is compared to that obtained in vertebrate striated muscle and is discussed on the basis of differences in Ca2+-regulation of the contractile system in these two muscles.

  3. Palliative sedation in advanced cancer patients: Does it shorten survival time? - A systematic review

    Directory of Open Access Journals (Sweden)

    B Barathi

    2013-01-01

    Full Text Available Background: Patients with advanced cancer often suffer from multiple refractory symptoms in the terminal phase of their life. Palliative sedation is one of the few ways to relieve this refractory suffering. Objectives: This systematic review investigated the effect of palliative sedation on survival time in terminally ill cancer patients. Materials and Methods: Six electronic databases were searched for both prospective and retrospective studies which evaluated the effect of palliative sedation on survival time. Only those studies which had a comparison group that did not receive palliative sedation were selected for the review. Abstracts of all retrieved studies were screened to include the most relevant studies and only studies which met inclusion criteria were selected. References of all retrieved studies were also screened for relevant studies. Selected studies were assessed for quality and data extraction was done using the structured data extraction form. Results: Eleven studies including four prospective and seven retrospective studies were identified. Mean survival time (MST was measured as the time from last admission until death. A careful analysis of the results of all the 11 studies indicated that MST of sedated and non-sedated group was not statistically different in any of the studies. Conclusion: This systematic review supports the fact that palliative sedation does not shorten survival in terminally ill cancer patients. However, this conclusion needs to be taken with consideration of the methodology, study design, and the population studied of the included studies in this review.

  4. Shortening distance of forward and reverse primers for nucleic acid isothermal amplification.

    Science.gov (United States)

    Haitao, Qu; Wenchao, Zhang; Xiaohui, Zhang; Xiujun, Wang; Sulong, Li

    2014-06-01

    Existent nucleic acid isothermal detection techniques for clinical diseases are difficult to promote greatly due to limitations in such aspects as methodology, costs of detection, amplification efficiency and conditions for operation. There is therefore an urgent need for a new isothermal amplification method with the characteristics of high accuracy, easy operation, short time of detection and low costs. We have devised a new method of nucleic acid isothermal amplification using Bst DNA polymerase under isothermal conditions (60-65°C). We call this method of amplification by shortening the distance between forward and reverse primers for nucleic acid isothermal amplification SDAMP. The results demonstrated that this technique is highly sensitive, specific and has short reaction times (40-60 min). Results of sequencing show that the products of SDAMP amplification are mainly polymers formed by series connection of monomers formed through linkage of forward primer and complementary sequences in reverse primer via a few bases. The method is different from current methods of nucleic acid amplification. Our study shows, however, that it is a specific method of nucleic acid isothermal amplification depending on interactions between primers and DNA template.

  5. Examining a scaled dynamical system of telomere shortening

    Science.gov (United States)

    Cyrenne, Benoit M.; Gooding, Robert J.

    2015-02-01

    A model of telomere dynamics is proposed and examined. Our model, which extends a previously introduced model that incorporates stem cells as progenitors of new cells, imposes the Hayflick limit, the maximum number of cell divisions that are possible. This new model leads to cell populations for which the average telomere length is not necessarily a monotonically decreasing function of time, in contrast to previously published models. We provide a phase diagram indicating where such results would be expected via the introduction of scaled populations, rate constants and time. The application of this model to available leukocyte baboon data is discussed.

  6. Subtrochanteric femoral shortening osteotomy combined with cementless total hip replacement for Crowe type IV developmental dysplasia: a retrospective study.

    Science.gov (United States)

    Rollo, Giuseppe; Solarino, Giuseppe; Vicenti, Giovanni; Picca, Girolamo; Carrozzo, Massimiliano; Moretti, Biagio

    2017-07-24

    Total hip replacement for high dislocation of the hip presents some difficulties, considering patients' young ages, the abnormal hip anatomy and the high rate of complications. In this study, we present our experience in terms of clinical and radiological results in the treatment of Crowe type IV hips with subtrochanteric femoral shortening osteotomy and cementless total hip replacement. We retrospectively reviewed 15 patients with Crowe type IV hip dysplasia (two bilateral cases for a total of 17 hips) treated with cementless total hip replacement associated with shortening subtrochanteric osteotomies (nine transversal and eight Z-shape osteotomies) between March 2000 to February 2006. The mean follow-up was 88 months (range 63-133). Harris hip score, leg length discrepancy, neurological status, union status of the osteotomy and the component stability were the criteria of the evaluation. All complications were noted. The mean HHS improved from 38.3 (range 32-52) to 85.6 (range 69-90). The mean preoperative leg length discrepancy was of 45 mm (range 38-70) and reduced to a mean of 12 mm (range 9-1.6) postoperatively. All osteotomies resulted healed at an average of 12.3 weeks (range 10-15). No cases of delayed union or nonunion were detected. Two patients (11%) showed early symptoms of sciatic nerve palsy which resolved uneventfully in 6 months. There was no migrations and none of the implants required revision. Cementless THA with shortening subtrochanteric osteotomy is an effective method in the treatment of patients with Crowe type IV development dysplasia of the hip. IV.

  7. Thermal lens spectrometry: Optimizing amplitude and shortening the transient time

    Science.gov (United States)

    Silva, Rubens; de Araújo, Marcos A. C.; Jali, Pedro; Moreira, Sanclayton G. C.; Alcantara, Petrus; de Oliveira, Paulo C.

    2011-06-01

    Based on a model introduced by Shen et al. for cw laser induced mode-mismatched dual-beam thermal lens spectrometry (TLS), we explore the parameters related with the geometry of the laser beams and the experimental apparatus that influence the amplitude and time evolution of the transient thermal lens (TL) signal. By keeping the sample cell at the minimum waist of the excitation beam, our results show that high amplitude TL signals, very close to the optimized value, combined with short transient times may be obtained by reducing the curvature radius of the probe beam and the distance between the sample cell and the detector. We also derive an expression for the thermal diffusivity which is independent of the excitation laser beam waist, considerably improving the accuracy of the measurements. The sample used in the experiments was oleic acid, which is present in most of the vegetable oils and is very transparent in the visible spectral range.

  8. Thermal lens spectrometry: Optimizing amplitude and shortening the transient time

    Directory of Open Access Journals (Sweden)

    Rubens Silva

    2011-06-01

    Full Text Available Based on a model introduced by Shen et al. for cw laser induced mode-mismatched dual-beam thermal lens spectrometry (TLS, we explore the parameters related with the geometry of the laser beams and the experimental apparatus that influence the amplitude and time evolution of the transient thermal lens (TL signal. By keeping the sample cell at the minimum waist of the excitation beam, our results show that high amplitude TL signals, very close to the optimized value, combined with short transient times may be obtained by reducing the curvature radius of the probe beam and the distance between the sample cell and the detector. We also derive an expression for the thermal diffusivity which is independent of the excitation laser beam waist, considerably improving the accuracy of the measurements. The sample used in the experiments was oleic acid, which is present in most of the vegetable oils and is very transparent in the visible spectral range.

  9. Spine biomechanics associated with the shortened, modern one-plane golf swing.

    Science.gov (United States)

    Dale, R Barry; Brumitt, Jason

    2016-06-01

    The purpose of this study was to compare kinetic, kinematic, and performance variables associated with full and shortened modern backswings in a skilled group of modern swing (one-plane) golfers. Shortening the modern golf backswing is proposed to reduce vertebral spine stress, but supporting evidence is lacking and performance implications are unknown. Thirteen male golfers performed ten swings of each swing type using their own 7-iron club. Biomechanical-dependent variables included the X-Factor kinematic data and spine kinetics. Performance-related dependent variables included club head velocity (CHV), shot distance, and accuracy (distance from the target line). Data were analysed with repeated measures ANOVA with an a priori alpha of 0.05 (SPSS 22.0, IBM, Armonk, NY, USA). We found significant reductions for the X-Factor (p < 0.05) between the full and shortened swings. The shortened swing condition ameliorated vertebral compression force from 7.6 ± 1.4 to 7.0 ± 1.7 N (normalised to body weight, p = 0.01) and significantly reduced CHV (p < 0.05) by ~2 m/s with concomitant shot distance diminution by ~10 m (p < 0.05). Further research is necessary to examine the applicability of a shortened swing for golfers with low back pain.

  10. The effect of muscle length on force depression after active shortening in soleus muscle of mice.

    Science.gov (United States)

    Van Noten, Pieter; Van Leemputte, Marc

    2011-07-01

    Isometric muscle force after active shortening is reduced [force depression (FD)]. The mechanism is incompletely understood but work delivered during shortening has been suggested to be the main determinant of FD. However, whether muscle length affects the sensitivity of FD to work is unknown, although this information might add to the understanding of the phenomenon. The aim of this study is to investigate the length dependence of the FD/work ratio (Q). Therefore, isometric force production (ISO) of 10 incubated mouse soleus muscles was compared to isometric force after 0.6, 1.2, and 2.4 mm shortening (IAS) at different end lengths ranging from L(0) - 3 to L(0) + 1.8 mm in steps of 0.6 mm. FD was calculated as the force difference between an ISO and IAS contraction at the same activation time (6 s) and end length. We confirm the strong relation between FD and work at L(0) (R² = 0.92) and found that FD is length dependent with a maximum of 8.8 ± 0.3% at L(0) + 1.2 mm for 0.6 mm shortening amplitude. Q was only constant for short muscle lengths (muscle length. The observed length dependence of Q indicates that FD is not only determined by work produced during shortening but also by a length-dependent factor, possibly actin compliance, which should be incorporated in any mechanism explaining FD.

  11. A new adaptive testing algorithm for shortening health literacy assessments

    Directory of Open Access Journals (Sweden)

    Currie Leanne M

    2011-08-01

    Full Text Available Abstract Background Low health literacy has a detrimental effect on health outcomes, as well as ability to use online health resources. Good health literacy assessment tools must be brief to be adopted in practice; test development from the perspective of item-response theory requires pretesting on large participant populations. Our objective was to develop a novel classification method for developing brief assessment instruments that does not require pretesting on large numbers of research participants, and that would be suitable for computerized adaptive testing. Methods We present a new algorithm that uses principles of measurement decision theory (MDT and Shannon's information theory. As a demonstration, we applied it to a secondary analysis of data sets from two assessment tests: a study that measured patients' familiarity with health terms (52 participants, 60 items and a study that assessed health numeracy (165 participants, 8 items. Results In the familiarity data set, the method correctly classified 88.5% of the subjects, and the average length of test was reduced by about 50%. In the numeracy data set, for a two-class classification scheme, 96.9% of the subjects were correctly classified with a more modest reduction in test length of 35.7%; a three-class scheme correctly classified 93.8% with a 17.7% reduction in test length. Conclusions MDT-based approaches are a promising alternative to approaches based on item-response theory, and are well-suited for computerized adaptive testing in the health domain.

  12. THE ETIOLOGICAL FACTORS INVOLVED IN DENTAL ARCH PERIMETER SHORTENING

    Directory of Open Access Journals (Sweden)

    Liliana Ioana Vâţă

    2012-12-01

    Full Text Available The main purpose of the study was to highlight the causes that may lead to reduction of arches: early loss of deciduous teeth, with consecutive dental drifting on the arch, followed by narrowing of the space necessary for the eruption of successional teeth, caries of the deciduous teeth or ectopic eruption of the first permanent molars. The mesial position of M1 had a frequency of 19.31% of the total number of analysed cases; maximum frequencies have been registered for the localization on the lower arch; M1 ectopia has rarely occurred on the studied batch; the frequency of lateral group mesial position was of 10.34% on the studied batch, registering maximum values for the localisation on the upper arch. The early loss of deciduous teeth was identified in almost 30% of the analysed cases, while the reduction of the space necessary for the eruption of successional teeth was registered in almost 39% of cases, space narrowing resulting both from the caries of deciduous teeth and from their early loss.

  13. Telomere shortening and cell senescence induced by perylene derivatives in A549 human lung cancer cells.

    Science.gov (United States)

    Taka, Thanachai; Huang, Liming; Wongnoppavich, Ariyaphong; Tam-Chang, Suk-Wah; Lee, T Randall; Tuntiwechapikul, Wirote

    2013-02-15

    Cancer cells evade replicative senescence by re-expressing telomerase, which maintains telomere length and hence chromosomal integrity. Telomerase inhibition would lead cancer cells to senesce and therefore prevent cancer cells from growing indefinitely. G-quadruplex ligands can attenuate telomerase activity by inducing G-quadruplex formation at the 3'-overhang of telomere and at the human telomerase reverse transcriptase (hTERT) promoter; the former prevents telomerase from accessing the telomere, and the latter acts as a transcriptional silencer. The present investigation found that perylene derivatives PM2 and PIPER induced G-quadruplex formation from both telomeric DNA and the hTERT promoter region in vitro. Further, TRAP assay showed that these compounds inhibited telomerase in a dose-dependent manner. When A549 human lung cancer cells were treated with these compounds, hTERT expression was down-regulated. Moreover, the crude protein extract from these treated cells exhibited less telomerase activity. In the long-term treatment of A549 lung cancer cells with sub-cytotoxic dose of these perylenes, telomere shortening, reduction of cell proliferation and tumorigenicity, and cell senescence were observed. The results of this study indicate that perylene derivatives warrant further consideration as effective agents for cancer therapy.

  14. Property improvement of pulsed laser deposited boron carbide films by pulse shortening

    Energy Technology Data Exchange (ETDEWEB)

    Csako, T. [Department of Optics and Quantum Electronics, University of Szeged, P.O. Box 406, H-6701 Szeged (Hungary); Budai, J. [Department of Optics and Quantum Electronics, University of Szeged, P.O. Box 406, H-6701 Szeged (Hungary); Szoerenyi, T. [Research Group on Laser Physics of the Hungarian Academy of Sciences, University of Szeged, P.O. Box 406, H-6701 Szeged (Hungary)]. E-mail: t.szorenyi@physx.u-szeged.hu

    2006-04-30

    Growth characteristics and surface morphology of boron carbide films fabricated by ablating a B{sub 4}C target in high vacuum with a traditional KrF excimer laser and a high brightness hybrid dye/excimer laser system emitting at the same wavelength while delivering 700 fs pulses are compared. The ultrashort pulse processing is highly effective. Energy densities between 0.25 and 2 J cm{sup -2} result in apparent growth rates ranging from 0.017 to 0.085 nm/pulse. Ablation with nanosecond pulses of one order of magnitude higher energy densities yields smaller growth rates, the figures increase from 0.002 to 0.016 nm/pulse within the 2-14.3 J cm{sup -2} fluence window. 2D thickness maps derived from variable angle spectroscopic ellipsometry reveal that, when ablating with sub-ps pulses, the spot size rather than the energy density determines both the deposition rate and the angular distribution of film material. Pulse shortening leads to significant improvement in surface morphology, as well. While droplets with number densities ranging from 1 x 10{sup 4} to 7 x 10{sup 4} mm{sup -2} deteriorate the surface of the films deposited by the KrF excimer laser, sub-ps pulses produce practically droplet-free films. The absence of droplets has also a beneficial effect on the stoichiometry and homogeneity of the films fabricated by ultrashort pulses.

  15. Response of tibialis anterior tendon to a chronic exposure of stretch-shortening cycles: age effects

    Directory of Open Access Journals (Sweden)

    Baker Brent B

    2009-06-01

    Full Text Available Abstract Background The purpose of the current study was to investigate the effects of aging on tendon response to repetitive exposures of stretch-shortening cycles (SSC's. Methods The left hind limb from young (3 mo, N = 4 and old (30 mo, N = 9 male Fisher 344 × Brown Norway rats were exposed to 80 maximal SSCs (60 deg/s, 50 deg range of motion 3x/week for 4.5 weeks in vivo. After the last exposure, tendons from the tibialis anterior muscle were isolated, stored at -80°C, and then tested using a micro-mechanical testing machine. Deformation of each tendon was evaluated using both relative grip-to-grip displacements and reference marks via a video system. Results At failure, the young control tendons had higher strain magnitude than the young exposed (p Conclusion The chronic protocol enhanced the elastic stiffness of young tendon and the loads in both the young and old tendons. The old exposed tendons were found to exhibit higher load capacity than their younger counterparts, which differed from our initial hypothesis.

  16. Life-shortening Wolbachia infection reduces population growth of Aedes aegypti.

    Science.gov (United States)

    Suh, Eunho; Mercer, David R; Dobson, Stephen L

    2017-08-01

    Wolbachia bacteria are being introduced into natural populations of vector mosquitoes, with the goal of reducing the transmission of human diseases such as Zika and dengue fever. The successful establishment of Wolbachia infection is largely dependent on the effects of Wolbachia infection to host fitness, but the effects of Wolbachia infection on the individual life-history traits of immature mosquitoes can vary. Here, the effects of life-shortening Wolbachia (wMelPop) on population growth of infected individuals were evaluated by measuring larval survival, developmental time and adult size of Aedes aegypti in intra- (infected or uninfected only) and inter-group (mixed with infected and uninfected) larval competition assays. At low larval density conditions, the population growth of wMelPop infected and uninfected individuals was similar. At high larval densities, wMelPop infected individuals had a significantly reduced population growth rate relative to uninfected individuals, regardless of competition type. We discuss the results in relation to the invasion of the wMelPop Wolbachia infection into naturally uninfected populations. Copyright © 2017 Elsevier B.V. All rights reserved.

  17. Shortened cortical silent period in adductor spasmodic dysphonia: evidence for widespread cortical excitability.

    Science.gov (United States)

    Samargia, Sharyl; Schmidt, Rebekah; Kimberley, Teresa Jacobson

    2014-02-07

    The purpose of this study was to compare cortical inhibition in the hand region of the primary motor cortex between subjects with focal hand dystonia (FHD), adductor spasmodic dysphonia (AdSD), and healthy controls. Data from 28 subjects were analyzed (FHD n=11, 53.25 ± 8.74 y; AdSD: n=8, 56.38 ± 7.5 y; and healthy controls: n=941.67 ± 10.85 y). All subjects received single pulse TMS to the left motor cortex to measure cortical silent period (CSP) in the right first dorsal interosseus (FDI) muscle. Duration of the CSP was measured and compared across groups. A one-way ANCOVA with age as a covariate revealed a significant group effect (p<0.001). Post hoc analysis revealed significantly longer CSP duration in the healthy group vs. AdSD group (p<0.001) and FHD group (p<0.001). These results suggest impaired intracortical inhibition is a neurophysiologic characteristic of FHD and AdSD. In addition, the shortened CSP in AdSD provides evidence to support a widespread decrease in cortical inhibition in areas of the motor cortex that represent an asymptomatic region of the body. These findings may inform future investigations of differential diagnosis as well as alternative treatments for focal dystonias.

  18. A diversity compression and combining technique based on channel shortening for cooperative networks

    KAUST Repository

    Hussain, Syed Imtiaz

    2012-02-01

    The cooperative relaying process with multiple relays needs proper coordination among the communicating and the relaying nodes. This coordination and the required capabilities may not be available in some wireless systems where the nodes are equipped with very basic communication hardware. We consider a scenario where the source node transmits its signal to the destination through multiple relays in an uncoordinated fashion. The destination captures the multiple copies of the transmitted signal through a Rake receiver. We analyze a situation where the number of Rake fingers N is less than that of the relaying nodes L. In this case, the receiver can combine N strongest signals out of L. The remaining signals will be lost and act as interference to the desired signal components. To tackle this problem, we develop a novel signal combining technique based on channel shortening principles. This technique proposes a processing block before the Rake reception which compresses the energy of L signal components over N branches while keeping the noise level at its minimum. The proposed scheme saves the system resources and makes the received signal compatible to the available hardware. Simulation results show that it outperforms the selection combining scheme. © 2012 IEEE.

  19. Line end shortening and application of novel correction algorithms in e-beam direct write

    Science.gov (United States)

    Freitag, Martin; Choi, Kang-Hoon; Gutsch, Manuela; Hohle, Christoph

    2011-03-01

    For the manufacturing of semiconductor technologies following the ITRS roadmap, we will face the nodes well below 32nm half pitch in the next 2~3 years. Despite being able to achieve the required resolution, which is now possible with electron beam direct write variable shaped beam (EBDW VSB) equipment and resists, it becomes critical to precisely reproduce dense line space patterns onto a wafer. This exposed pattern must meet the targets from the layout in both dimensions (horizontally and vertically). For instance, the end of a line must be printed in its entire length to allow a later placed contact to be able to land on it. Up to now, the control of printed patterns such as line ends is achieved by a proximity effect correction (PEC) which is mostly based on a dose modulation. This investigation of the line end shortening (LES) includes multiple novel approaches, also containing an additional geometrical correction, to push the limits of the available data preparation algorithms and the measurement. The designed LES test patterns, which aim to characterize the status of LES in a quick and easy way, were exposed and measured at Fraunhofer Center Nanoelectronic Technologies (CNT) using its state of the art electron beam direct writer and CD-SEM. Simulation and exposure results with the novel LES correction algorithms applied to the test pattern and a large production like pattern in the range of our target CDs in dense line space features smaller than 40nm will be shown.

  20. Pinealectomy shortens resynchronisation times of house sparrow ( Passer domesticus) circadian rhythms

    Science.gov (United States)

    Kumar, Vinod; Gwinner, Eberhard

    2005-09-01

    In many birds periodic melatonin secretion by the pineal organ is essential for the high-amplitude self-sustained output of the circadian pacemaker, and thus for the persistence of rhythmicity in 24 h oscillations controlled by it. The elimination of the pineal melatonin rhythm, or a reduction of its amplitude, renders the circadian pacemaker a less self-sustained, often highly damped, oscillatory system. A reduction in the degree of self-sustainment of a rhythm should not only increase its range of entrainment but also shorten the resynchronization times following phase-shifts of the zeitgeber. This hypothesis has not yet been directly tested. We therefore carried out the present study in which house sparrows (Passer domesticus) were subjected to both 6-h advance and 6-h delay phase-shifts of the light-dark cycle before and after the pinealectomy, and the rhythms in locomotion and feeding were recorded. The results indicate that following the delay, but not the advance, phase shift, resynchronization times were significantly shorter after pinealectomy. The dependence of resynchronization times on the presence or absence of the pineal organ is not only of theoretical interest but might also be of functional significance in the natural life of birds. A reduction or elimination of the amplitude of the melatonin secretion rhythm by the pineal organ might be responsible for faster adjustment to changes in zeitgeber conditions in nature.

  1. Tectonic shortening and coeval volcanism during the Quaternary, Northeast Japan arc

    Indian Academy of Sciences (India)

    Koji Umeda; Masao Ban; Shintaro Hayashi; Tomohiro Kusano

    2013-02-01

    The Northeast Japan arc, a mature volcanic arc with a back-arc marginal basin (Japan Sea), is located on a convergent plate boundary along the subducting Pacific plate and the overriding North American plate. From a compilation and analysis of stratigraphy, radiometric age and data on erupted magma volumes, 176 eruptive episodes identified from 69 volcanoes so far, indicate that notable changes in eruption style, magma discharge rates and distribution of eruptive centres occurred around 1.0 Ma. Before ca.1.0 Ma, large-volume felsic eruptions were dominant, forming large calderas in the frontal arc, a region of low crustal strain rate. After ca. 1.0 Ma to the present, the calc-alkaline andesite magma eruptions in the frontal and rear arcs, synchronous with crustal shortening characterized by reverse faulting, resulted in stratovolcano development along narrow uplifted zones. Although, it is widely assumed that magma cannot rise easily in a compressional setting, some of the magma stored within basal sills could be extruded where N–S-trending uplifted mountains bounded by reverse faults formed since about ca.1.0 Ma.

  2. A signal combining technique based on channel shortening for cooperative sensor networks

    KAUST Repository

    Hussain, Syed Imtiaz

    2010-06-01

    The cooperative relaying process needs proper coordination among the communicating and the relaying nodes. This coordination and the required capabilities may not be available in some wireless systems, e.g. wireless sensor networks where the nodes are equipped with very basic communication hardware. In this paper, we consider a scenario where the source node transmits its signal to the destination through multiple relays in an uncoordinated fashion. The destination can capture the multiple copies of the transmitted signal through a Rake receiver. We analyze a situation where the number of Rake fingers N is less than that of the relaying nodes L. In this case, the receiver can combine N strongest signals out of L. The remaining signals will be lost and act as interference to the desired signal components. To tackle this problem, we develop a novel signal combining technique based on channel shortening. This technique proposes a processing block before the Rake reception which compresses the energy of L signal components over N branches while keeping the noise level at its minimum. The proposed scheme saves the system resources and makes the received signal compatible to the available hardware. Simulation results show that it outperforms the selection combining scheme. ©2010 IEEE.

  3. Freezing shortens the lifetime of DNA molecules under tension.

    Science.gov (United States)

    Chung, Wei-Ju; Cui, Yujia; Chen, Chi-Shuo; Wei, Wesley H; Chang, Rong-Shing; Shu, Wun-Yi; Hsu, Ian C

    2017-09-08

    DNA samples are commonly frozen for storage. However, freezing can compromise the integrity of DNA molecules. Considering the wide applications of DNA molecules in nanotechnology, changes to DNA integrity at the molecular level may cause undesirable outcomes. However, the effects of freezing on DNA integrity have not been fully explored. To investigate the impact of freezing on DNA integrity, samples of frozen and non-frozen bacteriophage lambda DNA were studied using optical tweezers. Tension (5-35 pN) was applied to DNA molecules to mimic mechanical interactions between DNA and other biomolecules. The integrity of the DNA molecules was evaluated by measuring the time taken for single DNA molecules to break under tension. Mean lifetimes were determined by maximum likelihood estimates and variances were obtained through bootstrapping simulations. Under 5 pN of force, the mean lifetime of frozen samples is 44.3 min with 95% confidence interval (CI) between 36.7 min and 53.6 min while the mean lifetime of non-frozen samples is 133.2 min (95% CI: 97.8-190.1 min). Under 15 pN of force, the mean lifetimes are 10.8 min (95% CI: 7.6-12.6 min) and 78.5 min (95% CI: 58.1-108.9 min). The lifetimes of frozen DNA molecules are significantly reduced, implying that freezing compromises DNA integrity. Moreover, we found that the reduced DNA structural integrity cannot be restored using regular ligation process. These results indicate that freezing can alter the structural integrity of the DNA molecules.

  4. Lengthening and shortening of plasma DNA in hepatocellular carcinoma patients

    Science.gov (United States)

    Jiang, Peiyong; Chan, Carol W. M.; Chan, K. C. Allen; Cheng, Suk Hang; Wong, John; Wong, Vincent Wai-Sun; Wong, Grace L. H.; Chan, Stephen L.; Mok, Tony S. K.; Chan, Henry L. Y.; Lai, Paul B. S.; Chiu, Rossa W. K.; Lo, Y. M. Dennis

    2015-01-01

    The analysis of tumor-derived circulating cell-free DNA opens up new possibilities for performing liquid biopsies for the assessment of solid tumors. Although its clinical potential has been increasingly recognized, many aspects of the biological characteristics of tumor-derived cell-free DNA remain unclear. With respect to the size profile of such plasma DNA molecules, a number of studies reported the finding of increased integrity of tumor-derived plasma DNA, whereas others found evidence to suggest that plasma DNA molecules released by tumors might be shorter. Here, we performed a detailed analysis of the size profiles of plasma DNA in 90 patients with hepatocellular carcinoma, 67 with chronic hepatitis B, 36 with hepatitis B-associated cirrhosis, and 32 healthy controls. We used massively parallel sequencing to achieve plasma DNA size measurement at single-base resolution and in a genome-wide manner. Tumor-derived plasma DNA molecules were further identified with the use of chromosome arm-level z-score analysis (CAZA), which facilitated the studying of their specific size profiles. We showed that populations of aberrantly short and long DNA molecules existed in the plasma of patients with hepatocellular carcinoma. The short ones preferentially carried the tumor-associated copy number aberrations. We further showed that there were elevated amounts of plasma mitochondrial DNA in the plasma of hepatocellular carcinoma patients. Such molecules were much shorter than the nuclear DNA in plasma. These results have improved our understanding of the size profile of tumor-derived circulating cell-free DNA and might further enhance our ability to use plasma DNA as a molecular diagnostic tool. PMID:25646427

  5. Association between leukocyte telomere shortening and exposure to traffic pollution: a cross-sectional study on traffic officers and indoor office workers

    Directory of Open Access Journals (Sweden)

    Albetti Benedetta

    2009-09-01

    Full Text Available Abstract Background Telomere shortening in blood leukocytes has been associated with increased morbidity and death from cardiovascular disease and cancer, but determinants of shortened telomeres, a molecular feature of biological aging, are still largely unidentified. Traffic pollution has been linked with both cardiovascular and cancer risks, particularly in older subjects. Whether exposure to traffic pollution is associated with telomere shortening has never been evaluated. Methods We measured leukocyte telomere length (LTL by real-time PCR in blood DNA from 77 traffic officers exposed to high levels of traffic pollutants and 57 office workers (referents. Airborne benzene and toluene, as tracers for traffic exposure, were measured using personal passive samplers and gas-chromatography/flame-ionization detector analysis. We used covariate-adjusted multivariable models to test the effects of the exposure on LTL and obtain adjusted LTL means and 95% Confidence Intervals (CIs. Results Adjusted mean LTL was 1.10 (95%CI 1.04-1.16 in traffic officers and 1.27 in referents (95%CI 1.20-1.35 [p Conclusion Our results indicate that leukocyte telomere length is shortened in subjects exposed to traffic pollution, suggesting evidence of early biological aging and disease risk.

  6. Shortening of primary operators in N-extended $SCFT_{4}$ and harmonic-superspace analyticity

    CERN Document Server

    Andrianopoli, Laura; Sokatchev, Emery S; Zupnik, B M

    1999-01-01

    We present the analysis of all possible shortenings which occur for composite gauge invariant conformal primary superfields in SU(2,2/N) invariant gauge theories. These primaries have top-spin range N/2 \\leq J_{max} < N with J_{max} = J_1 + J_2, (J_1,J_2) being the SL(2,C) quantum numbers of the highest spin component of the superfield. In Harmonic superspace, analytic and chiral superfields give J_{max}= N/2 series while intermediate shortenings correspond to fusion of chiral with analytic in N=2, or analytic with different analytic structures in N=3,4. In the AdS/CFT language shortenings of UIR's correspond to all possible BPS conditions on bulk states. An application of this analysis to multitrace operators, corresponding to multiparticle supergravity states, is spelled out.

  7. [Comparison of force and shortening velocity in fast and slow rabbit muscle fibers at different temperatures].

    Science.gov (United States)

    Kochubeĭ, P V; Bershitskiĭ, S Iu

    2014-01-01

    The temperature dependence of force, maximal shortening velocity and power of maximally activated single permeabilized fibers from fast and slow muscles of the rabbit were recorded in a temperature range from 10 to 35 degrees C with 5 degrees C step. It was found that temperature dependence of force of both types of fibers is identical. Averaged maximal shortening velocity in the slow fibers, unlike the fast fibers, had no statistically significant temperature dependence that is not in agreement with the data obtained on intact rat muscle fibers and in an in vitro motility assay. However maximal shortening velocity in each individual slow fiber did depend on temperature. The temperature dependence of power of the slow fibers was lower than that of the fast ones. Because of large data scattering the average temperature dependence of power of the slow fibers was significantly lower than that in individual slow fibers.

  8. Assessment of muscle shortening and static posture in children with persistent asthma.

    Science.gov (United States)

    Lopes, Erica A; Fanelli-Galvani, Adriana; Prisco, Camilla C V; Gonçalves, Raquel C; Jacob, Cristina M A; Cabral, Anna L B; Martins, Milton A; Carvalho, Celso R F

    2007-07-01

    Asthmatic patients experience an increase in airway resistance that overburdens both respiratory and non-respiratory muscles. The objective of the present study was to determine whether children with persistent asthma present muscle shortening and postural changes. The 60 boys evaluated, aged 7-12 (pubertal ages up to Tanner stage G2) were divided into three age- and BMI-matched groups of equal number: CON (no history of asthma or allergy); MPA (mild persistent asthma); SPA (severe persistent asthma). Pulmonary function, muscle shortening and static posture were evaluated. The SPA group presented higher protraction of the head and shoulder compared with the CON group [9.5 (6.0-12.0) degrees vs 5.5 (0.0-12.0) degrees, P control subjects in five out nine evaluated outcomes. Our data suggest that severe asthmatic children present postural adaptations and muscle shortening that seem to be related to disease severity.

  9. Increased genomic alteration complexity and telomere shortening in B-CLL cells resistant to radiation-induced apoptosis

    Energy Technology Data Exchange (ETDEWEB)

    Salin, H.; Ricoul, M.; Morat, L.; Sabatier, L. [CEA, DSV, iRCM, LRO, F-92265 Fontenay Aux Roses (France); Salin, H. [Museum Natl Hist Nat, F-75231 Paris (France)

    2008-07-01

    B-cell chronic lymphocytic leukemia (B-CLL) results in an accumulation of mature CD5{sup +}/CD23{sup +} B cells due to an uncharacterised defect in apoptotic cell death. B-CLL is not characterized by a unique recurrent genomic alteration but rather by genomic instability giving rise frequently to several chromosomal aberrations. Besides we reported that similar to 15% of B-CLL patients present malignant B-cells resistant to irradiation-induced apoptosis, contrary to similar to 85% of patients and normal human lymphocytes. Telomere length shortening is observed in radioresistant B-CLL cells. Using fluorescence in situ hybridization (FISH) and multicolour FISH, we tested whether specific chromosomal aberrations might be associated with the radioresistance of a subset of B-CLL cells and whether they are correlated with telomere shortening. In a cohort of 30 B-CLL patients, all of the radioresistant B-CLL cell samples exhibited homozygous or heterozygous deletion of 13q14.3 in contrast to 52% of the radiosensitive samples. In addition to the 13q14.3 deletion, ten out of the 11 radioresistant B-cell samples had another clonal genomic alteration such as trisomy 12, deletion 17p13.1, mutation of the p53 gene or translocations in contrast to only three out of 19 radiosensitive samples. Telomere fusions and non-reciprocal translocations, hallmarks of telomere dysfunction, are not increased in radioresistant B-CLL cells. These findings suggest (i) that the 13q14.3 deletion accompanied by another chromosomal aberration is associated with radioresistance of B-CLL cells and (ii) that telomere shortening is not causative of increased clonal chromosomal aberrations in radioresistant B-CLL cells. (authors)

  10. Single rabbit stomach smooth muscle cell myosin heavy chain SMB expression and shortening velocity.

    Science.gov (United States)

    Eddinger, T J; Meer, D P

    2001-02-01

    Isolated single smooth muscle cells (SMCs) from different regions of the rabbit stomach were used to determine a possible correlation between unloaded shortening velocity and smooth muscle (SM) myosin heavy chain (MHC) S1 head isoform composition (SMA, no head insert; SMB, with head insert). alpha-Toxin-permeabilized isolated single cells were maximally activated to measure unloaded shortening velocity and subsequently used in an RT-PCR reaction to determine the SMA/SMB content of the same cell. SM MHC SMA and SMB isoforms are uniquely distributed in the stomach with cells from the fundic region expressing little SMB (38.1 +/- 7.3% SMB; n = 16); cells from the antrum express primarily SMB (94.9 +/- 1.0% SMB; n = 16). Mean fundic cell unloaded shortening velocity was 0.014 +/- 0.002 cell lengths/s compared with 0.036 +/- 0.002 for the antrum cells. Unloaded shortening velocity in these cells was significantly correlated with their percent SMB expression (r2 = 0.58). Resting cell length does not correlate with the percent SMB expression (n = 32 cells). Previously published assays of purified or expressed SMA and SMB heavy meromyosin show a twofold difference in actin filament sliding speed in in vitro motility assays. Extrapolation of our data to 0-100% SMB would give a 10-fold range of shortening velocity, which is closer to the approximately 20-fold range reported from various SM tissues. This suggests that mechanisms in addition to the MHC S1 head isoforms regulate shortening velocity.

  11. Shortening behavior of the different components of muscle-tendon unit during isokinetic plantar flexions.

    Science.gov (United States)

    Hauraix, Hugo; Nordez, Antoine; Dorel, Sylvain

    2013-10-01

    The torque-velocity relationship has been widely considered as reflecting the mechanical properties of the contractile apparatus, and the influence of tendinous tissues on this relationship obtained during in vivo experiments remains to be determined. This study describes the pattern of shortening of various muscle-tendon unit elements of the triceps surae at different constant angular velocities and quantifies the contributions of fascicles, tendon, and aponeurosis to the global muscle-tendon unit shortening. Ten subjects performed isokinetic plantar flexions at different preset angular velocities (i.e., 30, 90, 150, 210, 270, and 330°/s). Ultrafast ultrasound measurements were performed on the muscle belly and on the myotendinous junction of the medial and lateral gastrocnemius muscles. The contributions of fascicles, tendon, and aponeurosis to global muscle-tendon unit shortening velocity were calculated for velocity conditions for four parts of the total range of motion. For both muscles, the fascicles' contribution decreased throughout the motion (73.5 ± 21.5% for 100-90° angular range to 33.7 ± 20.2% for 80-70°), whereas the tendon contribution increased (25.8 ± 15.4 to 55.6 ± 16.8%). In conclusion, the tendon contribution to the global muscle-tendon unit shortening is significant even during a concentric contraction. However, this contribution depends on the range of motion analyzed. The intersubject variability found in the maximal fascicle shortening velocity, for a given angular velocity, suggests that some subjects might possess a more efficient musculoarticular complex to produce the movement velocity. These findings are of great interest for understanding the ability of muscle-tendon shortening velocity.

  12. In vivo maximal fascicle-shortening velocity during plantar flexion in humans.

    Science.gov (United States)

    Hauraix, Hugo; Nordez, Antoine; Guilhem, Gaël; Rabita, Giuseppe; Dorel, Sylvain

    2015-12-01

    Interindividual variability in performance of fast movements is commonly explained by a difference in maximal muscle-shortening velocity due to differences in the proportion of fast-twitch fibers. To provide a better understanding of the capacity to generate fast motion, this study aimed to 1) measure for the first time in vivo the maximal fascicle-shortening velocity of human muscle; 2) evaluate the relationship between angular velocity and fascicle-shortening velocity from low to maximal angular velocities; and 3) investigate the influence of musculo-articular features (moment arm, tendinous tissues stiffness, and muscle architecture) on maximal angular velocity. Ultrafast ultrasound images of the gastrocnemius medialis were obtained from 31 participants during maximal isokinetic and light-loaded plantar flexions. A strong linear relationship between fascicle-shortening velocity and angular velocity was reported for all subjects (mean R(2) = 0.97). The maximal shortening velocity (V(Fmax)) obtained during the no-load condition (NLc) ranged between 18.8 and 43.3 cm/s. V(Fmax) values were very close to those of the maximal shortening velocity (V(max)), which was extrapolated from the F-V curve (the Hill model). Angular velocity reached during the NLc was significantly correlated with this V(Fmax) (r = 0.57; P < 0.001). This finding was in agreement with assumptions about the role of muscle fiber type, whereas interindividual comparisons clearly support the fact that other parameters may also contribute to performance during fast movements. Nevertheless, none of the biomechanical features considered in the present study were found to be directly related to the highest angular velocity, highlighting the complexity of the upstream mechanics that lead to maximal-velocity muscle contraction.

  13. Markers of cellular senescence. Telomere shortening as a marker of cellular senescence.

    Science.gov (United States)

    Bernadotte, Alexandra; Mikhelson, Victor M; Spivak, Irina M

    2016-01-01

    The cellular senescence definition comes to the fact of cells irreversible proliferation disability. Besides the cell cycle arrest, senescent cells go through some morphological, biochemical, and functional changes which are the signs of cellular senescence. The senescent cells (including replicative senescence and stress-induced premature senescence) of all the tissues look alike. They are metabolically active and possess the set of characteristics in vitro and in vivo, which are known as biomarkers of aging and cellular senescence. Among biomarkers of cellular senescence telomere shortening is a rather elegant frequently used biomarker. Validity of telomere shortening as a marker for cellular senescence is based on theoretical and experimental data.

  14. Telomere shortening correlates to dysplasia but not to DNA aneuploidy in longstanding ulcerative colitis

    DEFF Research Database (Denmark)

    Friis-Ottessen, Mariann; Bendix, Laila; Kølvraa, Steen;

    2014-01-01

    Ulcerative colitis (UC) is a chronic, inflammatory bowel disease which may lead to dysplasia and adenocarcinoma in patients when long-lasting. Short telomeres have been reported in mucosal cells of UC patients. Telomeres are repetitive base sequences capping the ends of linear chromosomes......, and protect them from erosion and subsequent wrongful recombination and end-to-end joining during cell division. Short telomeres are associated with the development of chromosomal instability and aneuploidy, the latter being risk factors for development of dysplasia and cancer. Specifically, the abrupt...... shortening of one or more telomeres to a critical length, rather than bulk shortening of telomeres, seems to be associated with chromosomal instability....

  15. Diffusion tensor imaging of the human calf muscle: distinct changes in fractional anisotropy and mean diffusion due to passive muscle shortening and stretching.

    Science.gov (United States)

    Schwenzer, Nina F; Steidle, Günter; Martirosian, Petros; Schraml, Christina; Springer, Fabian; Claussen, Claus D; Schick, Fritz

    2009-12-01

    The influence of passive shortening and stretching of the calf muscles on diffusion characteristics was investigated. The diffusion tensor was measured in transverse slices through the lower leg of eight healthy volunteers (29 +/- 7 years) on a 3 T whole-body MR unit in three different positions of the foot (40 degrees plantarflexion, neutral ankle position (0 degrees ), and -10 degrees dorsiflexion in the ankle). Maps of the mean diffusivity, the three eigenvalues of the tensor and fractional anisotropy (FA) were calculated. Results revealed a distinct dependence of the mean diffusivity and FA on the foot position and the related shortening and stretching of the muscle groups. The tibialis anterior muscle showed a significant increase of 19% in FA with increasing dorsiflexion, while the FA of the antagonists significantly decreased ( approximately 20%). Regarding the mean diffusivity of the diffusion tensor, the muscle groups showed an opposed response to muscle elongation and shortening. Regarding the eigenvalues of the diffusion tensor, lambda(2) and lambda(3) showed significant changes in relation to muscle length. In contrast, no change in lambda(1) could be found. This work reveals significant changes in diffusional characteristics induced by passive muscle shortening and stretching.

  16. Brain targeted transcranial administration of diazepam and shortening of sleep latency in healthy human volunteers

    Directory of Open Access Journals (Sweden)

    W Pathirana

    2011-01-01

    Full Text Available Application of medicated oils on scalp had been practiced for centuries in the Ayurvedic system of medicine in diseases associated with the central nervous system. It is possible that the effectiveness of the therapy may be a result of targeted delivery of active compounds to the brain transcranially. Evidence also comes from two previous studies with positive results on brain targeted transcranial delivery of methadone base and diazepam on rat models. Possibility of transcranial drug delivery was investigated in healthy human volunteers using electroencephalography techniques by assessing the ability of transcranially administered diazepam in bringing about β activity in the electroencephalographic wave patterns and shortening of the sleep latency period. Non polar drug molecules dissolved in a non-aqueous sesame oil based vehicle is a significant feature in the transcranial dosage design. The study was under taken in two phases. In the Phase-I study scalp application of a single dose of 2 mg/3 ml of the oil was employed and in the Phase-II study repeat application of three doses 24 h apart were employed. Sleep latency changes were monitored with Multiple Sleep Latency Tests with 5 naps employing the standard electroencephalography, electroocculography and electromyography electrodes. Sleep onset was identified with the first epoch of any sleep stage non rapid eye movement 1, 2, 3, 4 or rapid eye movement using electroencephalography, electroocculography and electromyography criteria. In both phases of the study there was significant reduction in the sleep latencies. It was much more pronounced in the Phase-II study. None of the subjects however displayed beta activity in the electroencephalography. Sleep latency reduction following scalp application in both the phases are suggestive of transcranial migration of diazepam molecules to the receptor sites of the nerve tissue of the brain eliciting its pharmacological effect of sedation

  17. Shortened Conditioned Eyeblink Response Latency in Male but not Female Wistar-Kyoto Hyperactive Rats

    Science.gov (United States)

    Thanellou, Alexandra; Schachinger, Kira M.; Green, John T.

    2014-01-01

    Reductions in the volume of the cerebellum and impairments in cerebellar-dependent eyeblink conditioning have been observed in attention-deficit/hyperactivity disorder (ADHD). Recently, it was reported that subjects with ADHD as well as male spontaneously hypertensive rats (SHR), a strain that is frequently employed as an animal model in the study of ADHD, exhibit a parallel pattern of timing deficits in eyeblink conditioning. One criticism that has been posed regarding the validity of the SHR strain as an animal model for the study of ADHD is that SHRs are not only hyperactive but also hypertensive. It is conceivable that many of the behavioral characteristics seen in SHRs that seem to parallel the behavioral symptoms of ADHD are not solely due to hyperactivity but instead are the net outcome of the interaction between hyperactivity and hypertension. We used Wistar-Kyoto Hyperactive (WKHA) and Wistar-Kyoto Hypertensive (WKHT) rats (males and females), strains generated from recombinant inbreeding of SHRs and their progenitor strain, Wistar-Kyoto (WKY) rats, to compare eyeblink conditioning in strains that are exclusively hyperactive or hypertensive. We used a long-delay eyeblink conditioning task in which a tone conditioned stimulus was paired with a periorbital stimulation unconditioned stimulus (750-ms delay paradigm). Our results showed that WKHA and WKHT rats exhibited similar rates of conditioned response (CR) acquisition. However, WKHA males displayed shortened CR latencies (early onset and peak latency) in comparison to WKHT males. In contrast, female WKHAs and WKHTs did not differ. In subsequent extinction training, WKHA rats extinguished at similar rates in comparison to WKHT rats. The current results support the hypothesis of a relationship between cerebellar abnormalities and ADHD in an animal model of ADHD-like symptoms that does not also exhibit hypertension, and suggest that cerebellar-related timing deficits are specific to males. PMID:19485572

  18. Matters which shorten the ozone layer; Substances qui appauvrissent la couche d'ozone

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-12-01

    This document, proposed by the ministry of the national development and the environment, gives the list of the main texts relative to the matters which shorten the ozone layer and the evolution of the community regulations in this domain. The concerned matters are the Cfc and the HCFC production and use. (A.L.B.)

  19. The addition of lidocaine to bupivacaine does not shorten the duration of spinal anesthesia

    DEFF Research Database (Denmark)

    Jacobsen, Jon; Husum, Bent; Staffeldt, Henrik

    2011-01-01

    The duration of spinal anesthesia with bupivacaine is often too long for day surgery. A recent study of patients presenting for transurethral surgery suggested that the addition of a small amount of lidocaine to intrathecal hyperbaric bupivacaine could shorten the duration of the sensory and motor...

  20. Decayed/missing/filled teeth and shortened dental arches in Tanzanian adults.

    NARCIS (Netherlands)

    Sarita, P.T.N.; Witter, D.J.; Kreulen, C.M.; Matee, M.I.N.; Hof, M.A. van 't; Creugers, N.H.J.

    2004-01-01

    PURPOSE: This study assessed decayed/missing/filled teeth (DMFT), presence of occlusal units, and prevalence of shortened dental arches in a Tanzanian adult population. MATERIALS AND METHODS: The dental state of samples of the Tanzanian population was studied. Oral examinations were conducted on

  1. Self-regulation by the Dutch medical profession of medical behavior that potentially shortens life

    NARCIS (Netherlands)

    Griffiths, John; Krabbendam, H.; Ten Napel, H.M.

    2000-01-01

    One of the most striking features of the way in which the Dutch have gone about regulating euthanasia and other "medical behavior that potentially shortens Life (MBPS) the key role that self-regulation has played in the process of legal development.' The rules and procedures that govern euthanasia

  2. Recovery time of motor evoked potentials following lengthening and shortening muscle action in the tibialis anterior

    NARCIS (Netherlands)

    Tallent, J.; Goodall, S.; Hortobagyi, T.; Gibson, A. St Clair; French, D. N.; Howatson, G.

    2012-01-01

    Motor evoked potentials (MEP) at rest remain facilitated following an isometric muscle contraction. Because the pre-synaptic and post-synaptic control of shortening (SHO) and lengthening (LEN) contractions differs, the possibility exists that the recovery of the MEP is also task specific. The time c

  3. Corrective osteotomy for malunion of the distal radius - The effect of concomitant ulnar shortening osteotomy

    NARCIS (Netherlands)

    Oskam, J; Bongers, KM; Karthaus, AJM; Frima, AJ; Klasen, HJ

    1996-01-01

    Positive ulnar variance due to inadequate correction of radial length is a common disorder after radial corrective osteotomy. To avoid this complication we performed a combination of ulnar-shortening osteotomy and radial corrective osteotomy in 6 of 22 radial corrections. The indication for the comb

  4. Interlayer material transport during layer-normal shortening. Part I. The model

    NARCIS (Netherlands)

    Molen, I. van der

    1985-01-01

    To analyse mass-transfer during deformation, the case is considered of a multilayer experiencing a layer-normal shortening that is volume constant on the scale of many layers. Strain rate is homogeneously distributed on the layer-scale if diffusion is absent; when transport of matter between the

  5. Common Patterns of Congenital Lower Extremity Shortening: Diagnosis, Classification, and Follow-up.

    Science.gov (United States)

    Bedoya, Maria A; Chauvin, Nancy A; Jaramillo, Diego; Davidson, Richard; Horn, B David; Ho-Fung, Victor

    2015-01-01

    Congenital lower limb shortening is a group of relatively rare, heterogeneous disorders. Proximal focal femoral deficiency (PFFD) and fibular hemimelia (FH) are the most common pathologic entities in this disease spectrum. PFFD is characterized by variable degrees of shortening or absence of the femoral head, with associated dysplasia of the acetabulum and femoral shaft. FH ranges from mild hypoplasia to complete absence of the fibula with variable shortening of the tibia. The development of the lower limb requires complex and precise gene interactions. Although the etiologies of PFFD and FH remain unknown, there is a strong association between the two disorders. Associated congenital defects in the lower extremity are found in more than 50% of patients with PFFD, ipsilateral FH being the most common. FH also has a strong association with shortening and bowing of the tibia and with foot deformities such as absence of the lateral rays of the foot. Early diagnosis and radiologic classification of these abnormalities are imperative for appropriate management and surgical planning. Plain radiography remains the main diagnostic imaging modality for both PFFD and FH, and appropriate description of the osseous abnormalities seen on radiographs allows accurate classification, prognostic evaluation, and surgical planning. Minor malformations may commonly be misdiagnosed.

  6. Crossbridge and non-crossbridge contributions to force in shortening and lengthening muscle.

    Science.gov (United States)

    Ranatunga, K W; Roots, H; Pinniger, G J; Offer, G W

    2010-01-01

    Analysis of tension responses to ramp length changes in muscle can provide important information about the crossbridge cycle. During a ramp length change, the force response of an active muscle shows an early change in slope (the P₁ transition) followed by a later, gradual change in slope (the P₂ transition). Modeling shows that the first transition reflects the tension change associated with the crossbridge power stroke in shortening and with its reversal in lengthening; the reduction in slope at the second transition occurs when most of the crossbridges (myosin heads) that were attached at the start of the ramp become detached; the steady tension during shortening is borne mainly by post-stroke heads whereas tension during lengthening is borne mostly by pre-stroke heads. After the P₂ transition, the tension reaches a steady level in the model whereas in the experiments the tension continues to increase during lengthening or to decrease during shortening; this tension change is seen at a wide range of sarcomere lengths and even when active force is reduced by a myosin inhibitor. It appears that some non-crossbridge components in muscle fibers stiffen upon activation and contribute to the continued tension rise during lengthening; release of such tension leads to tension decline during shortening. Thus, non-crossbridge visco-elasticity in sarcomeres may also contribute to energy storage and release during in situ muscle function.

  7. Fiber type composition and maximum shortening velocity of muscles crossing the human shoulder.

    Science.gov (United States)

    Srinivasan, R C; Lungren, M P; Langenderfer, J E; Hughes, R E

    2007-03-01

    A study of the fiber type composition of fourteen muscles spanning the human glenohumeral joint was carried out with the purpose of determining the contribution of fiber types to overall muscle cross-sectional area (CSA) and to estimate the maximum shortening velocity (V(max)) of those muscles. Muscle biopsies were procured from 4 male cadavers (mean age 50) within 24 hr of death, snap frozen, mounted, and transversely sectioned (10 microm). Slides were stained for myofibrillar ATPase after alkaline preincubation. Photoimages were taken of defined areas (100 fibers) using the Bioquant system, and fiber type and CSA were measured from these images. Staining for mATPase produced three different fiber types: slow-oxidative (SO), fast-oxidative-glycolytic (FOG), and fast-glycolytic (FG). On average, the muscle fiber type composition ranged from 22 to 40% of FG, from 17 to 51% of FOG, and from 23 to 56% of SO. Twelve out of the 14 muscles had average SO proportions ranging from 35 to 50%. V(max) was calculated from the fiber type contribution relative to CSA and shortening velocity values taken from the literature. The maximum velocities of shortening presented here provide a physiological basis for the development of human shoulder musculoskeletal models suitable for predicting muscle forces for functionally relevant tasks encompassing conditions of muscle shortening and lengthening.

  8. The effect of hypoxia on shortening contractions in rat diaphragm muscle.

    NARCIS (Netherlands)

    Machiels, H.A.; Heijden, E. van der; Heunks, L.M.A.; Dekhuijzen, P.N.R.

    2001-01-01

    Hypoxia is known to reduce isometric contractile properties of isolated rat diaphragm bundles. Its effect on isotonic contractile properties (i.e. force-velocity relationship and power output) has not been studied. We hypothesized that hypoxia reduces velocity of shortening and consequently power ou

  9. Contribution of NHE-1 to cell length cardiac shortening of normal and failing rabbit myocytes

    NARCIS (Netherlands)

    M.M.G.J. van Borren; J.G. Zegers; A. Baartscheer; J.H. Ravesloot

    2006-01-01

    At the same intracellular pH (pH(i)) Na+/H+ exchange (NHE-1) fluxes of ventricular myocytes of hypertrophied failing hearts (HFH) are increased. We assessed how NHE-1 affected cell length shortening. pH(i) was measured fluorimetrically in resting and twitching (1 - 3 Hz)normal and HFH rabbit myocyte

  10. Changing Quality Controls: The Effects of Increasing Product Variety and Shortening Product Life Cycles

    NARCIS (Netherlands)

    J.D. van Iwaarden (Jos)

    2006-01-01

    textabstractIn many industries (e.g. cars, electronics, clothing) manufacturing complexity and unpredictability have increased in recent years because of increasing product variety and shortening product life cycles. At the same time, manufacturers in these industries appear to have more problems wi

  11. The Shortened Visuospatial Questionnaire for Children: A Useful Tool to Identify Students with Low Visuospatial Abilities

    Science.gov (United States)

    Fastame, Maria Chiara; Cherchi, Rossella; Penna, Maria Pietronilla

    2015-01-01

    The current research was aimed mainly at exploring the reliability of a short-screening tool developed to self-evaluate visuospatial abilities in children. We presented 290 Italian third, fourth, and fifth graders with the 16-item Shortened Visuospatial questionnaire and several objective measures of intellectual efficiency, such as Raven's…

  12. Stochastic modeling of length-dependent telomere shortening in Corvus monedula

    NARCIS (Netherlands)

    Grasman, J.; Salomons, H. M.; Verhulst, S.

    2011-01-01

    It was recently shown that, within individuals, longer telomeres shorten at a higher rate. This explorative study deals with a mathematical model of this process. It is a nonlinear differential equation describing length-dependent decrease that can be linked to a Poisson process. The model also take

  13. Stochastic modelling of length-dependent telomere shortening in Corvus monedula

    NARCIS (Netherlands)

    Grasman, J.; Salomons, H.M.; Verhulst, S.

    2011-01-01

    It was recently shown that, within individuals, longer telomeres shorten at a higher rate. This explorative study deals with a mathematical model of this process. It is a nonlinear differential equation describing length-dependent decrease that can be linked to a Poisson process. The model also take

  14. Decayed/missing/filled teeth and shortened dental arches in Tanzanian adults.

    NARCIS (Netherlands)

    Sarita, P.T.N.; Witter, D.J.; Kreulen, C.M.; Matee, M.I.N.; Hof, M.A. van 't; Creugers, N.H.J.

    2004-01-01

    PURPOSE: This study assessed decayed/missing/filled teeth (DMFT), presence of occlusal units, and prevalence of shortened dental arches in a Tanzanian adult population. MATERIALS AND METHODS: The dental state of samples of the Tanzanian population was studied. Oral examinations were conducted on 5,5

  15. Regional myocardial shortening in relation to graft-reactive hyperemia and flow after coronary bypass surgery

    NARCIS (Netherlands)

    R.W. Brower (Ronald); P.W.J.C. Serruys (Patrick); E. Bos (Egbert); J. Nauta (Jan)

    1979-01-01

    textabstractExtent of regional shortening of myocardium in areas newly perfused by bypass grafting was determined in 56 patients by a new technique employing four to six radiopaque markers sutured in pairs to the epicardium near the coronary anastomosis. Paradoxical systolic expansion (PSE) was mani

  16. Development of a Shortened Form of the Fennema-Sherman Mathematics Attitudes Scales.

    Science.gov (United States)

    Mulhern, Fiona; Rae, Gordon

    1998-01-01

    Data from 196 Irish school children were analyzed and used to develop a shortened version of the Fennema-Sherman Mathematics Attitudes Scales (E. Fennema and J. Sherman, 1976). Internal consistency estimates of the reliability of scores on the whole scale and each of the subscales of the original and short form were favorable. (SLD)

  17. Differential Telomere Shortening in Blood versus Arteries in an Animal Model of Type 2 Diabetes

    Directory of Open Access Journals (Sweden)

    Samira Tajbakhsh

    2015-01-01

    Full Text Available Vascular dysfunction is an early feature of diabetic vascular disease, due to increased oxidative stress and reduced nitric oxide (NO bioavailability. This can lead to endothelial cell senescence and clinical complications such as stroke. Cells can become senescent by shortened telomeres and oxidative stress is known to accelerate telomere attrition. Sirtuin 1 (SIRT1 has been linked to vascular health by upregulating endothelial nitric oxide synthase (eNOS, suppressing oxidative stress, and attenuating telomere shortening. Accelerated leukocyte telomere attrition appears to be a feature of clinical type 2 diabetes (T2D and therefore the telomere system may be a potential therapeutic target in preventing vascular complications of T2D. However the effect of T2D on vascular telomere length is currently unknown. We hypothesized that T2D gives rise to shortened leukocyte and vascular telomeres alongside reduced vascular SIRT1 expression and increased oxidative stress. Accelerated telomere attrition was observed in circulating leukocytes, but not arteries, in T2D compared to control rats. T2D rats had blunted arterial SIRT1 and eNOS protein expression levels which were associated with reduced antioxidant defense capacity. Our findings suggest that hyperglycemia and a deficit in vascular SIRT1 per se are not sufficient to prematurely shorten vascular telomeres.

  18. Bringing the School to the Children : Shortening the Path to EFA

    OpenAIRE

    Lehman, Douglas

    2003-01-01

    Recent education planning initiatives in West and Central Africa show that the path to EFA may be shortened considerably by reconsidering the way basic education is delivered in isolated rural communities. Since independence, education systems have been expanding rapidly and are now serving most of the easy-to-reach population. For progress to continue, the focus must be shifted toward the...

  19. Cementless total hip arthroplasty with modified oblique femoral shortening osteotomy in Crowe type IV congenital hip dislocation.

    Science.gov (United States)

    Kiliçoğlu, Onder İ; Türker, Mehmet; Akgül, Turgut; Yazicioğlu, Onder

    2013-01-01

    Midterm results of cementless total hip arthroplasty in patients with Crowe type IV congenital dislocation of the hip were evaluated. A modified oblique subtrochanteric shortening osteotomy was used in all patients. A cylindrical femoral stem was used in all patients to stabilize the osteotomy. Mean follow-up was 82 months in 20 hips of 16 patients. Mean Merle D'Aubigné pain score increased from 2.52 to 5.65 points, function score improved from 4.0 to 5.3 points, and mobility score improved from 3.95 to 5.35. Mean greater trochanter height relative to the estimated hip center was 6.8 ± 2.0 cm preoperatively and -1 ± 0.2 cm postoperatively. Complications were dislocations in 3 patients, which were successfully managed without redislocation and fracture of greater trochanter in 3 patients, which healed uneventfully in 2 but with residual Trendelenburg gait in one. Total hip arthroplasty with modified oblique subtrochanteric shortening osteotomy is an effective technique for the treatment for Crowe type IV hip dislocation.

  20. Intestinal Insulin Signaling Encodes Two Different Molecular Mechanisms for the Shortened Longevity Induced by Graphene Oxide in Caenorhabditis elegans

    Science.gov (United States)

    Zhao, Yunli; Yang, Ruilong; Rui, Qi; Wang, Dayong

    2016-04-01

    Graphene oxide (GO) has been shown to cause multiple toxicities in various organisms. However, the underlying molecular mechanisms for GO-induced shortened longevity are still unclear. We employed Caenorhabditis elegans to investigate the possible involvement of insulin signaling pathway in the control of GO toxicity and its underlying molecular mechanisms. Mutation of daf-2, age-1, akt-1, or akt-2 gene induced a resistant property of nematodes to GO toxicity, while mutation of daf-16 gene led to a susceptible property of nematodes to GO toxicity, suggesting that GO may dysregulate the functions of DAF-2/IGF-1 receptor, AGE-1, AKT-1 and AKT-2-mediated kinase cascade, and DAF-16/FOXO transcription factor. Genetic interaction analysis suggested the involvement of signaling cascade of DAF-2-AGE-1-AKT-1/2-DAF-16 in the control of GO toxicity on longevity. Moreover, intestinal RNA interference (RNAi) analysis demonstrated that GO reduced longevity by affecting the functions of signaling cascade of DAF-2-AGE-1-AKT-1/2-DAF-16 in the intestine. DAF-16 could also regulate GO toxicity on longevity by functioning upstream of SOD-3, which encodes an antioxidation system that prevents the accumulation of oxidative stress. Therefore, intestinal insulin signaling may encode two different molecular mechanisms responsible for the GO toxicity in inducing the shortened longevity. Our results highlight the key role of insulin signaling pathway in the control of GO toxicity in organisms.

  1. Drug-induced QT-interval shortening following antiepileptic treatment with oral rufinamide.

    Science.gov (United States)

    Schimpf, Rainer; Veltmann, Christian; Papavassiliu, Theano; Rudic, Boris; Göksu, Turgay; Kuschyk, Jürgen; Wolpert, Christian; Antzelevitch, Charles; Ebner, Alois; Borggrefe, Martin; Brandt, Christian

    2012-05-01

    The arrhythmogenic potential of short QT intervals has recently been highlighted in patients with a short QT syndrome. Drug-induced QT-interval prolongation is a known risk factor for ventricular tachyarrhythmias. However, reports on drug-induced QT-interval shortening are rare and proarrhythmic effects remain unclear. Recently, rufinamide, a new antiepileptic drug for the add-on treatment of Lennox-Gastaut syndrome, was approved in the European Union and the United States. Initial trials showed drug-induced QT-interval shortening. The aim of our study was to evaluate the effects of rufinamide on QT intervals in patients with difficult-to-treat epilepsies. Nineteen consecutive patients with Lennox-Gastaut syndrome and other epilepsy syndromes were included (n = 12 men; mean age 41 ± 12 years). QRS, QT, and T(peak)-T(end) intervals were analyzed before and during rufinamide treatment. The mean QT interval shortened significantly following rufinamide administration (QT interval 349 ± 23 ms vs 327 ± 17 ms; corrected QT interval 402 ± 22 ms vs 382 ± 16 ms; P = .002). T(peak)-T(end) intervals were 79 ± 17 ms before and 70 ± 20 ms on treatment (P = .07). The mean reduction of the corrected QT interval was 20 ± 18 ms. During follow-up (3.04 ± 1.09 years), no adverse events including symptomatic cardiac arrhythmias or sudden cardiac deaths were observed. QTc-interval shortening following oral rufinamide administration in a small patient group was not associated with significant clinical adverse effects. These observations notwithstanding, the ability of rufinamide to significantly shorten the QT interval portends a potential arrhythmogenic risk that may best be guarded against by periodic electrocardiographic recordings. Copyright © 2012 Heart Rhythm Society. Published by Elsevier Inc. All rights reserved.

  2. Residual force depression following muscle shortening is exaggerated by prior eccentric drop jump exercise.

    Science.gov (United States)

    Dargeviciute, Gintare; Masiulis, Nerijus; Kamandulis, Sigitas; Skurvydas, Albertas; Westerblad, Håkan

    2013-10-15

    We studied the relation between two common force modifications in skeletal muscle: the prolonged force depression induced by unaccustomed eccentric contractions, and the residual force depression (rFD) observed immediately after active shortening. We hypothesized that rFD originates from distortion within the sarcomeres and the extent of rFD: 1) correlates to the force and work performed during the shortening steps, which depend on sarcomeric integrity; and 2) is increased by sarcomeric disorganization induced by eccentric contractions. Nine healthy untrained men (mean age 26 yr) participated in the study. rFD was studied in electrically stimulated knee extensor muscles. rFD was defined as the reduction in isometric torque after active shortening compared with the torque in a purely isometric contraction. Eccentric contractions were performed as 50 repeated drop jumps with active deceleration to 90° knee angle, immediately followed by a maximal upward jump. rFD was assessed before and 5 min to 72 h after drop jumps. The series of drop jumps caused a prolonged force depression, which was about two times larger at 20-Hz than at 50-Hz stimulation. There was a significant correlation between increasing rFD and increasing mechanical work performed during active shortening both before and after drop jumps. In addition, a given rFD was obtained at a markedly lower mechanical work after drop jumps. In conclusion, the extent of rFD correlates to the mechanical work performed during active shortening. A series of eccentric contractions causes a prolonged reduction of isometric force. In addition, eccentric contractions exaggerate rFD, which further decreases muscle performance during dynamic contractions.

  3. An increase in telomere sister chromatid exchange in murine embryonic stem cells possessing critically shortened telomeres

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Yisong [ORNL; Giannone, Richard J [ORNL; Wu, Jun [ORNL; Gomez, Marla V [ORNL; Liu, Yie [ORNL

    2005-01-01

    Telomerase deficiency leads to a progressive loss of telomeric DNA that eventually triggers cell apoptosis in human primary cells during prolonged growth in culture. Rare survivors can maintain telomere length through either activation of telomerase or recombination-based telomere lengthening, and thus proliferate indefinitely. We have explored the possibility that telomeres may be maintained through telomere sister chromatid exchange (T-SCE) in murine telomere reverse transcriptase-deficient (mTert -/-) splenocytes and ES cells. Because telomerase deficiency leads to gradual loss of telomeric DNA in mTert -/- splenocytes and ES cells and eventually to chromosomes with telomere signal-free ends (SFEs), we examined these cell types for evidence of sister chromatid exchange at telomeres, and observed an increase in T-SCEs only in a subset of mTert -/- splenocytes or ES cells that possessed multiple SFEs. Furthermore, T-SCEs were more often detected in ES cells than in splenocytes that harbored a similar frequency of SFEs. In mTert heterozygous (mTert +/-) ES cells or splenocytes, which are known to exhibit a decrease in average telomere length but no SFEs, no increase in T-SCE was observed. In addition to T-SCE, other genomic rearrangements (i.e., SCE) were also significantly increased in mTert -/- ES cells possessing critically short telomeres, but not in splenocytes. Our results suggest that animals and cell culture differ in their ability to carry out genomic rearrangements as a means of maintaining telomere integrity when telomeres become critically shortened.

  4. Coral reef mesopredators switch prey, shortening food chains, in response to habitat degradation.

    Science.gov (United States)

    Hempson, Tessa N; Graham, Nicholas A J; MacNeil, M Aaron; Williamson, David H; Jones, Geoffrey P; Almany, Glenn R

    2017-04-01

    Diet specificity is likely to be the key predictor of a predator's vulnerability to changing habitat and prey conditions. Understanding the degree to which predatory coral reef fishes adjust or maintain prey choice, in response to declines in coral cover and changes in prey availability, is critical for predicting how they may respond to reef habitat degradation. Here, we use stable isotope analyses to characterize the trophic structure of predator-prey interactions on coral reefs of the Keppel Island Group on the southern Great Barrier Reef, Australia. These reefs, previously typified by exceptionally high coral cover, have recently lost much of their coral cover due to coral bleaching and frequent inundation by sediment-laden, freshwater flood plumes associated with increased rainfall patterns. Long-term monitoring of these reefs demonstrates that, as coral cover declined, there has been a decrease in prey biomass, and a shift in dominant prey species from pelagic plankton-feeding damselfishes to territorial benthic algal-feeding damselfishes, resulting in differences in the principal carbon pathways in the food web. Using isotopes, we tested whether this changing prey availability could be detected in the diet of a mesopredator (coral grouper, Plectropomus maculatus). The δ(13)C signature in grouper tissue in the Keppel Islands shifted from a more pelagic to a more benthic signal, demonstrating a change in carbon sources aligning with the change in prey availability due to habitat degradation. Grouper with a more benthic carbon signature were also feeding at a lower trophic level, indicating a shortening in food chains. Further, we found a decline in the coral grouper population accompanying a decrease in total available prey biomass. Thus, while the ability to adapt diets could ameliorate the short-term impacts of habitat degradation on mesopredators, long-term effects may negatively impact mesopredator populations and alter the trophic structure of coral reef

  5. Characteristics of myocardial postsystolic shortening in patients with coronary artery disease assessed by strain rate imaging

    Institute of Scientific and Technical Information of China (English)

    YANG Li; QIU Qiong; ZHANG Hui-zhong; XIA Jin-xi

    2007-01-01

    Background Postsystolic shortening (PSS) has been proposed as a marker of myocardial dysfunction. Strain rate imaging (SRI)is a novel ultrasonic technique, allowing reliable and noninvasive measurement of myocardial deformation.The purpose of this study was to investigate the characteristics of myocardial longitudinal PSS by SRI in ischemic and infarct myocardium in patients with coronary artery disease, and to explore its clinical applicability.Methods Eleven patients with angina pectoris, 21 patients with myocardial infarction and 20 healthy subjects were included in the study. Apical four-, three- and two-chamber views were displayed; and septal, lateral, anteroseptal,posterior, anterior and inferior walls of the left ventricle were scanned, respectively. PSS strain (εpss), the ratio of εpss and systolic strain (εpss/εsys), the ratio of εpss and maximum strain (εpss/εmax) and the duration of PSS (Tpss) in ischemic, infarct and normal myocardium were analyzed.Results PSS was found more frequent in the ischemic and infarct segments compared with the normal segments (39%vs 22% and 56% vs 22%, respectively; both P<0.01). It was even more frequent in the infarct segments than in the ischemic segments (56% vs 39%, P<0.01). The absolute magnitude of εpss, εpss/εsys, εpss/εmax were significantly larger and Tpss significantly longer in the ischemic and infarct segments compared with that in the normal myocardium (P<0.01).εpss/εsys, εpss/εmax were even larger and Tpss even longer in the infarct than in the ischemic segments (P<0.01).Conclusions PSS is a common and important feature of the ischemic and infarct myocardium. εpss, εpss/εsys, εpss/εmax and Tpss as measured by SRI may be promising markers for the quantitative assessment of regional myocardial dysfunction in patients with coronary artery disease. εpss/εsys, εpss/εmax and Tpss may be helpful in differentiating infarct from ischemic myocardium.

  6. Long-Term Outcome of Step-Cut Ulnar Shortening Osteotomy for Ulnar Impaction Syndrome.

    Science.gov (United States)

    Papatheodorou, Loukia K; Baratz, Mark E; Bougioukli, Sofia; Ruby, Tyler; Weiser, Robert W; Sotereanos, Dean G

    2016-11-02

    Extra-articular ulnar shortening osteotomy is a common procedure for the surgical treatment of ulnar impaction syndrome. Several techniques for this osteotomy have been developed to avoid the morbidity associated with a standard transverse osteotomy. However, these techniques require special instrumentation and are expensive. The purpose of this study was to evaluate the outcome of step-cut ulnar shortening osteotomy without special jigs for ulnar impaction syndrome. A retrospective study of 164 consecutive patients who underwent step-cut ulnar shortening osteotomy between 2000 and 2010 was performed. The long arm of the step-cut osteotomy was oriented in the coronal plane parallel to the long axis of the ulna. The short arms of the osteotomy were perpendicular to the long axis in the axial plane. Fixation was performed with a palmar 3.5-mm standard neutralization plate and a lag screw. The goal of the osteotomy was to reduce ulnar variance, which was assessed in all patients with pronated grip-view radiographs preoperatively and postoperatively. Preoperative ulnar variance ranged from +1 to +6 mm. All patients were followed for at least 24 months. Union of the osteotomy site was achieved at a mean of 8.2 weeks. The union rate was 98.8%. There were 2 cases of nonunion, which required additional surgery. The mean postoperative ulnar variance was +0.2 mm (range, -1 to +1.5 mm) after a mean overall ulnar shortening of 2.5 mm. All patients returned to their previous work, in a mean of 4 months. The plate was removed from 12 patients because of plate-related symptoms. No other complications were encountered. The step-cut ulnar shortening osteotomy provides ample bone-to-bone contact and simplifies control of rotation. Stable internal fixation with standard techniques allowed an early return to functional activities. Palmar placement of the plate diminishes the need for plate removal. This is a simple and less expensive technique for ulnar shortening that does not

  7. Shortening in the Central Andes at the transition to flat slab subduction

    Science.gov (United States)

    Safipour, R.; DeCelles, P. G.; Carrapa, B.; Kapp, P. A.; Gehrels, G. E.; Reiners, P. W.

    2011-12-01

    Shortening in the Central Andes is considered to decrease north and south of the apex of the Bolivian orocline, mainly owing to differences in the pre-existing stratigraphic architecture of the continental margin. Estimates of shortening in the central Andes of northern Argentina remain poorly documented, but are required for assessment of the regional kinematic history of the orogenic system. The problem is acute at the north to south transition from the high elevation Puna plateau to the lower elevation region of the Sierras Pampeanas intra-foreland block uplifts, which corresponds with a transition to a flat segment of the subducting Nazca plate. Although deformation in the Eastern Cordillera appears to have propagated forelandward from west to east, the trend in the Sierras Pampeanas is not clear from existing data. We mapped the structures along a roughly E-W transect at latitude 28°S in the Sierra de Las Planchadas of the northern Sierras Pampeanas to measure finite strain and kinematic indicators, and to develop a regional restorable cross section for measuring total shortening. We also sampled for low-T thermochronology in order to determine the timing of exhumation and inferred thrust propagation. A minimum of 40 km of shortening in the Sierra de Las Planchadas is estimated from our restored cross section. When added to the 20 km shortening documented in ranges to the east by previous studies, this brings the total minimum estimated shortening at this latitude to ~60 km. Apatite fission track ages from the hanging walls of thrusts are ~20 Ma, and apatite helium ages range from 10 Ma west of the range to 2.3 Ma in the Fiambalá basin which borders the range to the east. Cretaceous and Paleogene cooling ages are observed in ranges to the east of the Sierra de Las Planchadas, so the young cooling ages found in this study suggest that exhumation was complex and possibly diachronous at this latitude. To the extent that exhumation occurs simultaneously with

  8. Serotonin in the solitary tract nucleus shortens the laryngeal chemoreflex in anaesthetized neonatal rats.

    Science.gov (United States)

    Donnelly, William T; Bartlett, Donald; Leiter, J C

    2016-07-01

    What is the central question of this study? Failure to terminate apnoea and arouse is likely to contribute to sudden infant death syndrome (SIDS). Serotonin is deficient in the brainstems of babies who died of SIDS. Therefore, we tested the hypothesis that serotonin in the nucleus of the solitary tract (NTS) would shorten reflex apnoea. What is the main finding and its importance? Serotonin microinjected into the NTS shortened the apnoea and respiratory inhibition associated with the laryngeal chemoreflex. Moreover, this effect was achieved through a 5-HT3 receptor. This is a new insight that is likely to be relevant to the pathogenesis of SIDS. The laryngeal chemoreflex (LCR), an airway-protective reflex that causes apnoea and bradycardia, has long been suspected as an initiating event in the sudden infant death syndrome. Serotonin (5-HT) and 5-HT receptors may be deficient in the brainstems of babies who die of sudden infant death syndrome, and 5-HT seems to be important in terminating apnoeas directly or in causing arousals or as part of the process of autoresuscitation. We hypothesized that 5-HT in the brainstem would limit the duration of the LCR. We studied anaesthetized rat pups between 7 and 21 days of age and made microinjections into the cisterna magna or into the nucleus of the solitary tract (NTS). Focal, bilateral microinjections of 5-HT into the caudal NTS significantly shortened the LCR. The 5-HT1a receptor antagonist, WAY 100635, did not affect the LCR consistently, nor did a 5-HT2 receptor antagonist, ketanserin, alter the duration of the LCR. The 5-HT3 specific agonist, 1-(3-chlorophenyl)-biguanide, microinjected bilaterally into the caudal NTS significantly shortened the LCR. Thus, endogenous 5-HT released within the NTS may curtail the respiratory depression that is part of the LCR, and serotonergic shortening of the LCR may be attributed to activation of 5-HT3 receptors within the NTS. 5-HT3 receptors are expressed presynaptically on C

  9. Strain data recorded in Xinjiang and their bearing on Tianshan’s uplift/shortening and Tarim basin’s rotation

    Directory of Open Access Journals (Sweden)

    Jiang Jingxiang

    2011-02-01

    Full Text Available Based on the continuous strain data recorded in Xinjiang since 1985, we discuss the mechanisms of Tianshan’s uplift and Tarim basin’s clockwise rotation. The results indicate; 1 The principal – compression directions in Tianshan are nearly NS, and their intersection angles with regional structures and mountains are nearly perpendicular, which is in accordance with Tianshan’s uplift and crustal shortening. 2 The principal compressions around Tarim basin tend to facilitate the regional faults’ left-lateral strike-slip movements and the basin’s clockwise rotation. These phenomena of uplift/shortening and rotation are fundamentally the results of India plate’s northward push on Euro-Asia plate, and the associated Pamir arc’s rapid northward movement and regional blocks’ interaction.

  10. Protein degradation and post-deboning tenderization in broiler breast meat with different degrees of muscle shortening

    Science.gov (United States)

    Deboning broiler breast fillets prior to rigor mortis negatively influences tenderness due to sarcomere shortening. The effects of sarcomere shortening on muscle protein degradation and breast meat tenderization during post-deboning aging are not well understood. The objective of this study was to m...

  11. Stretch-shortening cycle muscle power in women and men aged 18-81 years

    DEFF Research Database (Denmark)

    Edwén, C E; Thorlund, Jonas Bloch; Magnusson, Stig Peter;

    2014-01-01

    This study explored the age-related deterioration in stretch-shortening cycle (SSC) muscle power and concurrent force-velocity properties in women and men across the adult life span. A total of 315 participants (women: n = 188; men: n = 127) aged 18-81 years performed maximal countermovement jump...... on an instrumented force plate. Maximal SSC leg extension power expressed per kg body mass (Ppeak) was greater in men than in women across the adult age span (P ......This study explored the age-related deterioration in stretch-shortening cycle (SSC) muscle power and concurrent force-velocity properties in women and men across the adult life span. A total of 315 participants (women: n = 188; men: n = 127) aged 18-81 years performed maximal countermovement jumps...

  12. Characterization of an ultrafast Bragg-Switch for shortening hard x-ray pulses

    Science.gov (United States)

    Sander, M.; Koc, A.; Kwamen, C. T.; Michaels, H.; Reppert, A. v.; Pudell, J.; Zamponi, F.; Bargheer, M.; Sellmann, J.; Schwarzkopf, J.; Gaal, P.

    2016-11-01

    We present a nanostructured device that functions as photoacoustic hard x-ray switch. The device is triggered by femtosecond laser pulses and allows for temporal gating of hard x-rays on picosecond (ps) timescales. It may be used for pulse picking or even pulse shortening in 3rd generation synchrotron sources. Previous approaches mainly suffered from insufficient switching contrasts due to excitation-induced thermal distortions. We present a new approach where thermal distortions are spatially separated from the functional switching layers in the structure. Our measurements yield a switching contrast of 14, which is sufficient for efficient hard x-ray pulse shortening. The optimized structure also allows for utilizing the switch at high repetition rates of up to 208 kHz.

  13. Reconstruction of neglected developmental dysplasia by total hip arthroplasty with subtrochanteric shortening osteotomy.

    Science.gov (United States)

    Atilla, Bülent

    2016-03-01

    Patients with neglected developmental dysplasia (DDH) face with early osteoarthritis of the hip, limb length inequality and marked disability while total hip reconstruction is the only available choice.DDH has severe morphologic consequences, with distorted bony anatomy and soft tissue contractures around the hip. It is critical to evaluate patients thoroughly before surgery.Anatomic reconstruction at the level of true acetabulum with uncemented implant is the mainstay of treatment. This requires a subtrochanteric shortening osteotomy, which can be realised using different osteotomy and fixation options.Although a demanding technique with a high rate of related complications, once anatomic reconstruction of the hip is achieved, patients have a remarkably good functional capacity and implant survival during long follow-up periods. Cite this article: Atilla B. Reconstruction of neglected developmental dysplasia by total hip arthroplasty with subtrochanteric shortening osteotomy. EFORT Open Rev 2016;1:65-71. DOI: 10.1302/2058-5241.1.000026.

  14. A critical examination of the maximum velocity of shortening used in simulation models of human movement.

    Science.gov (United States)

    Domire, Zachary J; Challis, John H

    2010-12-01

    The maximum velocity of shortening of a muscle is an important parameter in musculoskeletal models. The most commonly used values are derived from animal studies; however, these values are well above the values that have been reported for human muscle. The purpose of this study was to examine the sensitivity of simulations of maximum vertical jumping performance to the parameters describing the force-velocity properties of muscle. Simulations performed with parameters derived from animal studies were similar to measured jump heights from previous experimental studies. While simulations performed with parameters derived from human muscle were much lower than previously measured jump heights. If current measurements of maximum shortening velocity in human muscle are correct, a compensating error must exist. Of the possible compensating errors that could produce this discrepancy, it was concluded that reduced muscle fibre excursion is the most likely candidate.

  15. Surveying of the deformed terraces and crust shortening rate in the northwestern Tarim Basin: Comment

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    @@ The paper of Shen et al., entitled "Surveying of the deformed terraces and crust shortening rate in the northwest Tarim Basin", was published in Chinese Science Bulletin (Vol. 46, No. 12)[1]. Shen et al. found the deformation of Late Pleistocene to Holocene terraces of the Boguzi River across the Artushi Anticline in the northwest Tarim Basin close to the Pamir, and made level survey and differential GPS measurement, which is of great importance to geodynamics for research on the coupling of Tianshan Mountains uplifting and Tarim Basin depression. But their understanding to the deformation mechanics of terraces and the calculation methods of crustal shortening are open to discussion. Therefore, we discuss it with Shen Jun et al.

  16. Foreland shortening and crustal balancing in the Andes at 30°S latitude

    Science.gov (United States)

    Allmendinger, R. W.; Figueroa, D.; Synder, D.; Beer, J.; Mpodozis, C.; Isaacks, B. L.

    1990-08-01

    Excellent surface exposures, known Benioff zone geometry, a dynamic morphology, and the availability of industry seismic reflection data all make the Andes at 30°S an excellent transect for investigating crustal-scale balanced sections. 150-170 km of horizontal shortening has occurred in three major belts located between the trench and the foreland. The thin-skinned, east-verging Precordillera of western Argentina accounts for 60-75% of the total shortening and formed mostly since major volcanism ceased at ˜10 Ma. Industry seismic reflection data show that the décollement of the Precordillera belt is located anomalously deep at ˜15 km. The belt is dominated by fault propagation folds and contains several prominent out-of-sequence thrust faults. Seismic stratigraphie analysis shows that Miocene strata in the Iglesia Valley, located between the Precordillera and the crest of the Andes, accumulated in a piggy-back basin. Onlap relations on the western side indicate that the High Cordillera was uplifted as a major fault bend fold over a buried ramp. Thrusting in the two western belts, both in the High Cordillera of Chile, formed during the waning stages of arc volcanism, 11-16 Ma. and account for 25-40% of the shortening. The observed shortening is probably greater than can be accounted for with reasonable crustal thicknesses, indicating the possibility of continental truncation or erosion along the plate margin or an anomalously thick root held down by the nearly flat subducted Nazca Plate. Our preferred crustal geometry puts the ramp between upper and lower crustal deformation west of the high topography, requiring crustal scale tectonic wedging to thicken the crust beneath the crest of the Andes. This non-unique model provides a simple explanation of the first order morphology of the Andes at this latitude.

  17. Influence of sex on performance fatigability of the plantar flexors following repeated maximal dynamic shortening contractions.

    Science.gov (United States)

    Lanning, Amelia C; Power, Geoffrey A; Christie, Anita D; Dalton, Brian H

    2017-10-01

    The purpose was to determine sex differences in fatigability during maximal, unconstrained velocity, shortening plantar flexions. The role of time-dependent measures (i.e., rate of torque development, rate of velocity development, and rate of neuromuscular activation) in such sex-related differences was also examined. By task termination, females exhibited smaller reductions in power and similar changes in rate of neuromuscular activation than males, indicating females were less fatigable than males.

  18. Shortened Length of Stay Improves Financial Outcomes in Living Donor Kidney Transplantation

    Science.gov (United States)

    Villa, Manuel; Siskind, Eric; Sameyah, Emil; Alex, Asha; Blum, Mark; Tyrell, Richard; Fana, Melissa; Mishler, Marni; Godwin, Andrew; Kuncewitch, Michael; Alexander, Mohini; Israel, Ezra; Bhaskaran, Madhu; Calderon, Kellie; Jhaveri, Kenar D.; Sachdeva, Mala; Bellucci, Alessandro; Mattana, Joseph; Fishbane, Steven; Coppa, Gene; Molmenti, Ernesto

    2013-01-01

    Kidney transplantation is the preferred clinical and most cost-effective option for end-stage renal disease. Significant advances have taken place in the care of the transplant patients with improvements in clinical outcomes. The optimization of the costs of transplantation has been a constant goal as well. We present herein the impact in financial outcomes of a shortened length of stay after kidney transplant. PMID:24436592

  19. Influence of the heart rate on mean circumferential shortening velocity: echocardiographic study of 183 normal subjects.

    Science.gov (United States)

    Mangiarotti, R; Martinotti, R; Monzani, V; Sardella, F; Pierini, A; Pastori, M; Randazzo, A

    1986-01-01

    Echocardiography was used to explore the influence of independent variables (age, body surface area and heart rate) on the mean circumferential shortening velocity (MVCF) in 183 healthy subjects. Multiple stepwise regression analysis shows that heart rate is the only variable of the three just mentioned that influences MVCF. A regression equation is evolved and proposed as an index of MVCF correction for varying heart rates.

  20. Disappearance of spasticity after selective dorsal rhizotomy does not prevent muscle shortening in children with cerebral palsy: a case report.

    Science.gov (United States)

    Spijker, Margje; Strijers, Rob L M; van Ouwerkerk, Willem J R; Becher, Jules G

    2009-05-01

    Selective dorsal rhizotomy is an effective treatment for spasticity in children with cerebral palsy who have a spastic motor disorder. It is hypothesized that muscle shortening is related to spasticity; the lack of stretch of a muscle is thought to be the cause of muscle shortening. If this is true, the treatment for spasticity should prevent the occurrence of muscle shortening during growth. We present the case of 1 child with cerebral palsy and spastic diplegia, for whom the treatment with selective dorsal rhizotomy was successful in improving the walking abilities. She did, however, develop muscle shortening during growth. In conclusion, the development of muscle shortening during growth in children with cerebral palsy and spastic paresis cannot be prevented by treatment for the spasticity alone.

  1. Bax-induced apoptosis shortens the life span of DNA repair defect Ku70-knockout mice by inducing emphysema.

    Science.gov (United States)

    Matsuyama, Shigemi; Palmer, James; Bates, Adam; Poventud-Fuentes, Izmarie; Wong, Kelvin; Ngo, Justine; Matsuyama, Mieko

    2016-06-01

    Cells with DNA damage undergo apoptosis or cellular senescence if the damage cannot be repaired. Recent studies highlight that cellular senescence plays a major role in aging. However, age-associated diseases, including emphysema and neurodegenerative disorders, are caused by apoptosis of lung alveolar epithelial cells and neurons, respectively. Therefore, enhanced apoptosis also promotes aging and shortens the life span depending on the cell type. Recently, we reported that ku70(-) (/) (-)bax(-) (/) (-) and ku70(-) (/) (-)bax(+/) (-) mice showed significantly extended life span in comparison with ku70(-) (/) (-)bax(+/+) mice. Ku70 is essential for non-homologous end joining pathway for DNA double strand break repair, and Bax plays an important role in apoptosis. Our study suggests that Bax-induced apoptosis has a significant impact on shortening the life span of ku70(-) (/) (-) mice, which are defective in one of DNA repair pathways. The lung alveolar space gradually enlarges during aging, both in mouse and human, and this age-dependent change results in the decrease of respiration capacity during aging that can lead to emphysema in more severe cases. We found that emphysema occurred in ku70(-) (/) (-) mice at the age of three-months old, and that Bax deficiency was able to suppress it. These results suggest that Bax-mediated apoptosis induces emphysema in ku70(-) (/) (-) mice. We also found that the number of cells, including bronchiolar epithelial cells and type 2 alveolar epithelial cells, shows a higher DNA double strand break damage response in ku70 KO mouse lung than in wild type. Recent studies suggest that non-homologous end joining activity decreases with increased age in mouse and rat model. Together, we hypothesize that the decline of Ku70-dependent DNA repair activity in lung alveolar epithelial cells is one of the causes of age-dependent decline of lung function resulting from excess Bax-mediated apoptosis of lung alveolar epithelial cells (and their

  2. Relative shortening velocity in locomotor muscles: turkey ankle extensors operate at low V/V(max).

    Science.gov (United States)

    Gabaldón, Annette M; Nelson, Frank E; Roberts, Thomas J

    2008-01-01

    The force-velocity properties of skeletal muscle have an important influence on locomotor performance. All skeletal muscles produce less force the faster they shorten and typically develop maximal power at velocities of approximately 30% of maximum shortening velocity (V(max)). We used direct measurements of muscle mechanical function in two ankle extensor muscles of wild turkeys to test the hypothesis that during level running muscles operate at velocities that favor force rather than power. Sonomicrometer measurements of muscle length, tendon strain-gauge measurements of muscle force, and bipolar electromyographs were taken as animals ran over a range of speeds and inclines. These measurements were integrated with previously measured values of muscle V(max) for these muscles to calculate relative shortening velocity (V/V(max)). At all speeds for level running the V/V(max) values of the lateral gastrocnemius and the peroneus longus were low (muscles were capable of producing 90% of peak isometric force but only 35% of peak isotonic power. V/V(max) increased in response to the demand for mechanical power with increases in running incline and decreased to negative values to absorb energy during downhill running. Measurements of integrated electromyograph activity indicated that the volume of muscle required to produce a given force increased from level to uphill running. This observation is consistent with the idea that V/V(max) is an important determinant of locomotor cost because it affects the volume of muscle that must be recruited to support body weight.

  3. Response of slow and fast muscle to hypothyroidism: maximal shortening velocity and myosin isoforms

    Science.gov (United States)

    Caiozzo, V. J.; Herrick, R. E.; Baldwin, K. M.

    1992-01-01

    This study examined both the shortening velocity and myosin isoform distribution of slow- (soleus) and fast-twitch (plantaris) skeletal muscles under hypothyroid conditions. Adult female Sprague-Dawley rats were randomly assigned to one of two groups: control (n = 7) or hypothyroid (n = 7). In both muscles, the relative contents of native slow myosin (SM) and type I myosin heavy chain (MHC) increased in response to the hypothyroid treatment. The effects were such that the hypothyroid soleus muscle expressed only the native SM and type I MHC isoforms while repressing native intermediate myosin and type IIA MHC. In the plantaris, the relative content of native SM and type I MHC isoforms increased from 5 to 13% and from 4 to 10% of the total myosin pool, respectively. Maximal shortening velocity of the soleus and plantaris as measured by the slack test decreased by 32 and 19%, respectively, in response to hypothyroidism. In contrast, maximal shortening velocity as estimated by force-velocity data decreased only in the soleus (-19%). No significant change was observed for the plantaris.

  4. Circular and longitudinal muscles shortening indicates sliding patterns during peristalsis and transient lower esophageal sphincter relaxation.

    Science.gov (United States)

    Patel, Nirali; Jiang, Yanfen; Mittal, Ravinder K; Kim, Tae Ho; Ledgerwood, Melissa; Bhargava, Valmik

    2015-09-01

    Esophageal axial shortening is caused by longitudinal muscle (LM) contraction, but circular muscle (CM) may also contribute to axial shortening because of its spiral morphology. The goal of our study was to show patterns of contraction of CM and LM layers during peristalsis and transient lower esophageal sphincter (LES) relaxation (TLESR). In rats, esophageal and LES morphology was assessed by histology and immunohistochemistry, and function with the use of piezo-electric crystals and manometry. Electrical stimulation of the vagus nerve was used to induce esophageal contractions. In 18 healthy subjects, manometry and high frequency intraluminal ultrasound imaging during swallow-induced esophageal contractions and TLESR were evaluated. CM and LM thicknesses were measured (40 swallows and 30 TLESRs) as markers of axial shortening, before and at peak contraction, as well as during TLESRs. Animal studies revealed muscular connections between the LM and CM layers of the LES but not in the esophagus. During vagal stimulated esophageal contraction there was relative movement between the LM and CM. Human studies show that LM-to-CM (LM/CM) thickness ratio at baseline was 1. At the peak of swallow-induced contraction LM/CM ratio decreased significantly (2). The pattern of contraction of CM and LM suggests sliding of the two muscles. Furthermore, the sliding patterns are in the opposite direction during peristalsis and TLESR.

  5. Shortened Lifespan and Lethal Hemorrhage in a Hemophilia A Mouse Model.

    Directory of Open Access Journals (Sweden)

    Janice M Staber

    Full Text Available Hemophilia A animal models have helped advance our understanding of factor VIII deficiency. Previously, factor VIII deficient mouse models were reported to have a normal life span without spontaneous bleeds. However, the bleeding frequency and survival in these animals has not been thoroughly evaluated.To investigate the survival and lethal bleeding frequency in two strains of E-16 hemophilia A mice.We prospectively studied factor VIII deficient hemizygous affected males (n = 83 and homozygous affected females (n = 55 for survival and bleeding frequency. Animals were evaluated for presence and location of bleeds as potential cause of death.Hemophilia A mice had a median survival of 254 days, which is significantly shortened compared to wild type controls (p < 0.0001. In addition, the hemophilia A mice experienced hemorrhage in several tissues. This previously-underappreciated shortened survival in the hemophilia A murine model provides new outcomes for investigation of therapeutics and also reflects the shortened lifespan of patients if left untreated.

  6. Crustal shortening and structural architecture of the Interandean and Subandean zones of southern Bolivia (21°S): Constraints from a new balanced cross section

    Science.gov (United States)

    Anderson, R. B.; Long, S. P.; Horton, B. K.; Calle, A.; Ramirez, V.

    2015-12-01

    Structural insights obtained from balanced cross sections, including thrust belt geometry, location of footwall ramps, and crustal shortening estimates, provide key information for testing model predictions of orogen dynamics (e.g., Cordilleran cyclicity, critical taper theory). New results from geologic mapping along an east-west transect in the central Andes are integrated with existing geophysical data to construct a balanced cross section across the Interandean (IAZ) and Subandean (SAZ) zones of southern Bolivia at 21°S, in order to define thrust belt geometry and estimate crustal shortening. The IAZ consists of a doubly vergent zone of 2-4 km-thick thrust sheets of mainly Silurian-Devonian rocks, which are structurally elevated ~10 km relative to equivalent SAZ levels to the east. Notably, our proposed IAZ geometry differs from published geometries that lack significant west-directed backthrusts. The SAZ is defined by regional-scale, fault-bend folds (10-20 km wavelength, 4-6 km amplitude) that exhume rocks as deep as Carboniferous above a 10-12 km-deep regional décollement in Silurian rocks. Previous studies have interpreted IAZ and SAZ shortening to be balanced by slip on two separate basement megathrust sheets at depth. We estimate 151 km (44%) of total east-west shortening in the IAZ (71 km) and SAZ (80 km), which is similar to a previous estimate (144 km, 42%). Importantly, our estimate of SAZ shortening restores the leading edge of the basement thrust sheet feeding displacement into the SAZ back to a corresponding footwall ramp that is constrained by a seismic reflection profile 90 km along strike to the south. Our shortening magnitudes are similar to nearby estimates to the north and south, which range between 60-86 km for the SAZ and 43-96 km for the IAZ. Future work will continue the cross section westward into the Eastern Cordillera hinterland, and explore potential variations in the geometry and style of basement deformation.

  7. The randomized shortened dental arch study (RaSDA: design and protocol

    Directory of Open Access Journals (Sweden)

    Kern Matthias

    2010-02-01

    Full Text Available Abstract Background Various treatment options for the prosthetic treatment of jaws where all molars are lost are under discussion. Besides the placement of implants, two main treatment types can be distinguished: replacement of the missing molars with removable dental prostheses and non-replacement of the molars, i.e. preservation of the shortened dental arch. Evidence is lacking regarding the long-term outcome and the clinical performance of these approaches. High treatment costs and the long time required for the treatment impede respective clinical trials. Methods/design This 14-center randomized controlled investigator-initiated trial is ongoing. Last patient out will be in 2010. Patients over 35 years of age with all molars missing in one jaw and with at least both canines and one premolar left on each side were eligible. One group received a treatment with removable dental prostheses for molar replacement (treatment A. The other group received a treatment limited to the replacement of all missing anterior and premolar teeth using fixed bridges (treatment B. A pilot trial with 32 patients was carried out. Two hundred and fifteen patients were enrolled in the main trial where 109 patients were randomized for treatment A and 106 for treatment B. The primary outcome measure is further tooth loss during the 5-year follow-up. The secondary outcome measures encompassed clinical, technical and subjective variables. The study is funded by the Deutsche Forschungsgemeinschaft (German Research Foundation, DFG WA 831/2-1, 2-2, 2-3, 2-4, 2-5. Discussion The particular value of this trial is the adaptation of common design components to the very specific features of complex dental prosthetic treatments. The pilot trial proved to be indispensable because it led to a number of adjustments in the study protocol that considerably improved the practicability. The expected results are of high clinical relevance and will show the efficacy of two common

  8. Shortening treatment time in robotic radiosurgery using a novel node reduction technique

    Energy Technology Data Exchange (ETDEWEB)

    Water, Steven van de; Hoogeman, Mischa S.; Breedveld, Sebastiaan; Heijmen, Ben J. M. [Department of Radiation Oncology, Erasmus MC-Daniel den Hoed Cancer Center, Groene Hilledijk 301, 3075 EA Rotterdam (Netherlands)

    2011-03-15

    Purpose: The fraction duration of robotic radiosurgery treatments can be reduced by generating more time-efficient treatment plans with a reduced number of node positions, beams, and monitor units (MUs). Node positions are preprogramed locations where the robot can position the focal spot of the x-ray beam. As the time needed for the robot to travel between node positions takes up a large part of the treatment time, the aim of this study was to develop and evaluate a node reduction technique in order to reduce the treatment time per fraction for robotic radiosurgery. Methods: Node reduction was integrated into the inverse planning algorithm, developed in-house for the robotic radiosurgery modality. It involved repeated inverse optimization, each iteration excluding low-contribution node positions from the planning and resampling new candidate beams from the remaining node positions. Node reduction was performed until the exclusion of a single node position caused a constraint violation, after which the shortest treatment plan was selected retrospectively. Treatment plans were generated with and without node reduction for two lung cases of different complexity, one oropharyngeal case and one prostate case. Plan quality was assessed using the number of node positions, beams and MUs, and the estimated treatment time per fraction. All treatment plans had to fulfill all clinical dose constraints. Extra constraints were added to maintain the low-dose conformality and restrict skin doses during node reduction. Results: Node reduction resulted in 12 residual node positions, on average (reduction by 77%), at the cost of an increase in the number of beams and total MUs of 28% and 9%, respectively. Overall fraction durations (excluding patient setup) were shortened by 25% (range of 18%-40%), on average. Dose distributions changed only little and dose in low-dose regions was effectively restricted by the additional constraints. Conclusions: The fraction duration of robotic

  9. Control of pre-Cenozoic extensional heterogeneities on the kinematics of Cenozoic shortening: Northwestern Argentina

    Science.gov (United States)

    Pearson, D. M.; Kapp, P. A.; Reiners, P. W.; Gehrels, G. E.

    2012-12-01

    Results from regional-scale mapping and structural analysis, (U-Th)/He and U-Pb age dating of apatite and zircon, and a regional balanced cross section allow us to build upon the pre-existing geological framework for the retroarc of northwestern Argentina. Coupled with previous work in the region, our results suggest that the style, distribution, and kinematics of shortening in the Eastern Cordillera and Santa Bárbara tectonomorphic provinces of the thrust belt at this latitude (24-25°S) were strongly influenced by the architecture of a Mesozoic rift system. In contrast to the thin-skinned Subandean fold-thrust belt of southern Bolivia where structures are primarily W-dipping and propagated progressively eastward through time, reverse faults in northwestern Argentina are more variably oriented, steeply E-dipping, locally inverted structures that propagated in an overall eastward direction in a much more sporadic manner. The first major eastward propagation event at this latitude occurred at ~40 Ma and is expressed as Eocene (U-Th)/He apatite and zircon ages coincident with the western arm of the Cretaceous Salta rift system. Other workers also documented angular unconformities of this age in the western and eastern arms of the rift system. Up until ~10 Ma, deformation and exhumation were restricted to the western margin of the Cretaceous rift in the western Eastern Cordillera and eastern margin of the Puna plateau. This was followed by an ~75 km jump in the location of the thrust front toward the east to bypass the central portion of a horst block of the Cretaceous rift system, followed by subsequent initiation of new E-dipping reverse faults in a subsystem that propagated toward the west into this pre-existing structural high. During Pliocene time, deformation again migrated >100 km eastward to a Cretaceous syn-rift depocenter in the Santa Bárbara Ranges and was likely followed by additional westward propagation to the current location of deformation with the

  10. A comparative study of bone shortening and bone loss with use of saw blades versus burr in hallux valgus surgery.

    Science.gov (United States)

    Shahid, Muhammad Saleem; Lee, Paul; Evans, Sam; Thomas, Rhys

    2012-09-01

    The aim of this study was to assess bone loss and thickness of the cut with the use of a burr in percutaneous hallux valgus surgery. Twelve blocks of saw bone were used. Cuts of same depth were made in each block with two different saw blades and a burr. Each block was assessed for bone loss and thickness of the cut. There was statistically significant (ptwo different saw blades. The use of a burr resulted in threefold increased loss of bone material and fourfold increase in the thickness of the cut as compare to use of two different saw blades. The metatarsal shortening is a risk factor in percutaneous hallux valgus surgery with the use of a burr. Copyright © 2011 European Foot and Ankle Society. Published by Elsevier Ltd. All rights reserved.

  11. Discovery of high potency, single-chain insulin analogs with a shortened B-chain and nonpeptide linker.

    Science.gov (United States)

    Kaur, Zachary P; Ochman, Alexander R; Mayer, John P; Gelfanov, Vasily M; DiMarchi, Richard D

    2013-08-16

    A series of novel, single chain insulin analogs containing polyethylene glycol based connecting segments were synthesized by native chemical ligation and tested for biological activity. While the full length single chain insulin analogs exhibited low potency, deletion of amino acids B26-B30 unexpectedly generated markedly higher activity. This observation is unprecedented in all previous studies of single chain insulin analogs and is consistent with the presumption that in the native hormone this sequence must translocate to achieve high potency insulin receptor interaction. Optimization of the sequence yielded an insulin analog with potency and selectivity comparable to that of native insulin. These results establish a basis for discovery of novel higher potency, single chain insulin analogs of shortened length.

  12. The influence of loading intensity on muscle-tendon unit behavior during maximal knee extensor stretch shortening cycle exercise.

    Science.gov (United States)

    Earp, Jacob E; Newton, Robert U; Cormie, Prue; Blazevich, Anthony J

    2014-01-01

    Tendon stiffness increases as the magnitude and rate of loading increases, according to its viscoelastic properties. Thus, under some loading conditions tendons should become exceptionally stiff and act almost as rigid force transducers. Nonetheless, observations of tendon behavior during multi-joint sprinting and jumping tasks have shown that tendon strain increases whilst muscle strain decreases as the loading intensity increases. The purpose of the current study was to examine the influence of external loading intensity on muscle-tendon unit (MTU) behavior during a high-speed single-joint, stretch-shortening cycle (SSC) knee extension task. Eighteen men (n = 9) and women (n = 9) performed single-leg, maximum intensity SSC knee extensions at loads of 20, 60 and 90% of their one repetition maximum. Vastus lateralis fascicle length (L(f)) and velocity (v(f)) as well as MTU (L(MTU)) and tendinous tissue (L(t)) length were measured using high-speed ultrasonography (96 Hz). Patellar tendon force (F(t)) and rate of force development (RFDt) were estimated using inverse dynamics. Results showed that as loading intensity increased, concentric joint velocity and shortening v f decreased whilst F t and RFDt increased, but no significant differences were observed in eccentric joint velocity or peak L(MTU) or L(f). In addition, the tendon lengthened significantly less at the end of the eccentric phase at heavier loads. This is the first observation that tendon strain decreases significantly during a SSC movement as loading intensity increases in vivo, resulting in a shift in the tendon acting as a power amplifier at light loads to a more rigid force transducer at heavy loads.

  13. Effectivity of Betel Leaf (Piper betle L. Gel Extract in Shortening Bleeding Time After Deciduous Tooth Extraction

    Directory of Open Access Journals (Sweden)

    Regina Tedjasulaksana

    2017-01-01

    Full Text Available Background: As an Indonesian traditional medicine, betel leafis often used to stop nosebleed. Effective substances in betel leaves which serves to stop the bleeding is tannin. Objective: The aim of this study was to determine the effectiveness of the betel leaf ethanol extract gel shortened bleeding time after the revocation of deciduous teeth. Method: This research was conducted at the Department of Dental Nursing Clinic, Health Polytechnic Denpasar.This study is pure experimental research design with Completely Randomized Post Test Only Control Group Design. The total sample of 27 respondents were divided into a treatment group and two control groups. Anterior deciduous teeth on the physiological loose grade 3 or 4 is extracted, then the tooth socket is put pure gel for group 1 to group 2, epinephrine gel and gel ethanol extract of betel leaf for group 3. The bleeding time is calculated from the first moment the blood out until there is blood on filter paper that is placed on the tooth socket. Data were statistically analyzed with descriptive test and comparability test with One Way Anova. Result: The results showed bleeding time pure gel groups differ significantly with epinephrine group and the group of ethanol extract of betel leaf gel (p< 0.05. Bleeding time of epinephrine group did not differ significantly with betel leaf ethanol extract group (p>0.05. Conclusion: This means ethanol gel betel leaf extract can shorten bleeding after deciduous tooth extraction and it is suggested that the use of gel ethanol extract of betel leaves to cope with bleeding after tooth extraction.

  14. Dyslipidemia and chronic inflammation markers are correlated with telomere length shortening in Cushing's syndrome.

    Directory of Open Access Journals (Sweden)

    Anna Aulinas

    Full Text Available Cushing's syndrome (CS increases cardiovascular risk (CVR and adipocytokine imbalance, associated with an increased inflammatory state. Telomere length (TL shortening is a novel CVR marker, associated with inflammation biomarkers. We hypothesized that inflammatory state and higher CVR in CS might be related to TL shortening, as observed in premature aging.To evaluate relationships between TL, CVR and inflammation markers in CS.In a cross-sectional study, 77 patients with CS (14 males, 59 pituitary-, 17 adrenal- and 1 ectopic-origin; 21 active disease and 77 age-, gender-, smoking-matched controls were included. Total white blood cell TL was measured by TRF-Southern technique. Clinical data and blood samples were collected (lipids, adrenal function, glucose. Adiponectin, interleukin-6 (IL6 and C-reactive protein (CRP were available in a subgroup of patients (n=32. Correlations between TL and clinical features were examined and multiple linear regression analysis was performed to investigate potential predictors of TL.Dyslipidemic CS had shorter TL than non-dyslipidemic subjects (7328±1274 vs 7957±1137 bp, p<0.05. After adjustment for age and body mass index, cured and active CS dyslipidemic patients had shorter TL than non-dyslipidemic CS (cured: 7187±1309 vs 7868±1104; active: 7203±1262 vs 8615±1056, respectively, p<0.05. Total cholesterol and triglycerides negatively correlated with TL (r-0.279 and -0.259, respectively, p<0.05, as well as CRP and IL6 (r-0.412 and -0.441, respectively, p<0.05. No difference in TL according the presence of other individual CVR factors (hypertension, diabetes mellitus, obesity were observed in CS or in the control group. Additional TL shortening was observed in dyslipidemic obese patients who were also hypertensive, compared to those with two or less CVR factors (6956±1280 vs 7860±1180, respectively, p<0.001. Age and dyslipidemia were independent negative predictors of TL.TL is shortened in dyslipidemic CS

  15. Telomeres shorten more slowly in slow-aging wild animals than in fast-aging ones.

    Science.gov (United States)

    Dantzer, Ben; Fletcher, Quinn E

    2015-11-01

    Research on the physiological causes of senescence aim to identify common physiological mechanisms that explain age-related declines in fitness across taxonomic groups. Telomeres are repetitive nucleotide sequences found on the ends of eukaryotic chromosomes. Past research indicates that telomere attrition is strongly correlated with inter-specific rates of aging, though these studies cannot distinguish whether telomere attrition is a cause or consequence of the aging process. We extend previous research on this topic by incorporating recent studies to test the hypothesis that telomeres shorten more slowly with age in slow-aging animals than in fast-aging ones. We assembled all studies that have quantified cross-sectional (i.e. between-individual) telomere rates of change (TROC) over the lifespans of wild animals. This included 22 estimates reflecting absolute TROC (TROCabs, bp/yr, primarily measured using the terminal restriction fragment length method), and 10 estimates reflecting relative TROC (TROCrel, relative telomere length/yr, measured using qPCR), from five classes (Aves, Mammalia, Bivalvia, Reptilia, and Actinopterygii). In 14 bird species, we correlated between-individual (i.e. cross-sectional) TROCabs estimates with both maximum lifespan and a phylogenetically-corrected principle component axis (pcPC1) that reflected the slow-fast axis of life-history variation. Bird species characterized by faster life-histories and shorter maximum lifespans had faster TROCabs. In nine studies, both between-individual and within-individual TROC estimates were available (n=8 for TROCabs, n=1 for TROCrel). Within-individual TROC estimates were generally greater than between-individual TROC estimates, which is indicative of selective disappearance of individuals with shorter telomeres. However, the difference between within- and between-individual TROC estimates was only significant in two out of nine studies. The relationship between within-individual TROCabs and maximum

  16. Enhancement of Skeletal Muscle in Aged Rats Following High-Intensity Stretch-Shortening Contraction Training.

    Science.gov (United States)

    Rader, Erik P; Naimo, Marshall A; Layner, Kayla N; Triscuit, Alyssa M; Chetlin, Robert D; Ensey, James; Baker, Brent A

    2016-08-03

    Exercise is the most accessible, efficacious, and multifactorial intervention to improve health and treat chronic disease. High-intensity resistance exercise, in particular, also maximizes skeletal muscle size and strength-outcomes crucial at advanced age. However, such training is capable of inducing muscle maladaptation when misapplied at old age. Therefore, characterization of parameters (e.g., mode and frequency) that foster adaptation is an active research area. To address this issue, we utilized a rodent model that allowed training at maximal intensity in terms of muscle activation and tested the hypothesis that muscles of old rats adapt to stretch-shortening contraction (SSC) training, provided the training frequency is sufficiently low. At termination of training, normalized muscle mass (i.e., muscle mass divided by tibia length) and muscle quality (isometric force divided by normalized muscle mass) were determined. For young rats, normalized muscle mass increased by ∼20% regardless of training frequency. No difference was observed for muscle quality values after 2 days versus 3 days per week training (0.65 ± 0.09 N/mg/mm vs. 0.59 ± 0.05 N/mg/mm, respectively). For old rats following 3 days per week training, normalized muscle mass was unaltered and muscle quality was 30% lower than young levels. Following 2 days per week training at old age, normalized muscle mass increased by 17% and muscle quality was restored to young levels. To investigate this enhanced response, oxidative stress was assessed by lipid peroxidation quantification. For young rats, lipid peroxidation levels were unaltered by training. With aging, baseline levels of lipid peroxidation increased by 1.5-fold. For old rats, only 2 days per week training decreased lipid peroxidation to levels indistinguishable from young values. These results imply that, appropriately scheduled high-intensity SSC training at old age is capable of restoring muscle to a younger phenotype in terms

  17. Association between shortened telomere length and systemic lupus erythematosus: a meta-analysis.

    Science.gov (United States)

    Lee, Y H; Jung, J H; Seo, Y H; Kim, J-H; Choi, S J; Ji, J D; Song, G G

    2017-03-01

    Objective We aimed to evaluate the relationship between telomere length and systemic lupus erythematosus (SLE). Methods PUBMED and EMBASE databases were searched; meta-analyses were performed comparing telomere length in SLE patients and healthy controls, and on SLE patients in subgroups based on ethnicity, sample type, assay method and data type. Results Eight studies including 472 SLE patients and 365 controls were ultimately selected which showed that telomere length was significantly shorter in the SLE group than in the control group (standardized mean difference (SMD) = -0.835, 95% confidence interval (CI) = -1.291 to -0.380, p = 3.3 × 10(-4)). Stratification by ethnicity showed significantly shortened telomere length in the SLE group in Caucasian, Asian and mixed populations (SMD = -0.455, 95% CI = -0.763 to -0.147, p = 0.004; SMD = -0.887, 95% CI = -1.261 to -0.513, p = 3.4 × 10(-4); SMD = -0.535, 95% CI = -0.923 to -0.147, p = 0.007; respectively). Furthermore, telomere length was significantly shorter in the SLE group than in the control group in whole blood and peripheral blood mononuclear cell groups (SMD = -0.361, 95% CI = -0.553 to -0.169, p = 2.3 × 10(-4); SMD = -1.546, 95% CI = -2.583 to -0.510, p = 0.003; respectively); a similar trend was observed in leukocyte groups (SMD = -0.699, 95% CI = -1.511 to -0.114, p = 0.092). Meta-analyses based on assay method or data type revealed similar associations. Conclusions Our meta-analysis demonstrated that telomere length was significantly shorter in patients with SLE, regardless of ethnicity, sample type or assay method evaluated.

  18. Improvement of visual debugging tool. Shortening the elapsed time for getting data and adding new functions to compare/combine a set of visualized data

    Energy Technology Data Exchange (ETDEWEB)

    Matsuda, Katsuyuki; Takemiya, Hiroshi

    2001-03-01

    The visual debugging tool 'vdebug' has been improved, which was designed for the debugging of programs for scientific computing. Improved were the following two points; (1) shortening the elapsed time required for getting appropriate data to visualize; (2) adding new functions which enable to compare and/or combine a set of visualized data originated from two or more different programs. As for shortening elapsed time for getting data, with the improved version of 'vdebug', we could achieve the following results; over hundred times shortening the elapsed time with dbx, pdbx of SX-4 and over ten times with ndb of SR2201. As for the new functions to compare/combine visualized data, it was confirmed that we could easily checked the consistency between the computational results obtained in each calculational steps on two different computers: SP and ONYX. In this report, we illustrate how the tool 'vdebug' has been improved with an example. (author)

  19. Early Cenozoic Shortening and Foreland Basin Sedimentation in the Marañon Fold-thrust Belt, Central Peruvian Andes

    Science.gov (United States)

    Jackson, L. J.; Carlotto, V.; Horton, B. K.; Rosell, L. N.

    2015-12-01

    The Marañon fold-thrust belt in the westernmost Andes of Peru has long been considered a robust signature of early Cenozoic shortening in the Andean orogenic belt. However, the structural details and potential records of coeval synorogenic sedimentation remain elusive. We report results from new geologic mapping (1:50,000), cross-section construction, and U-Pb geochronology for the Matucana-Ticlio region at 11-12°S along the Lima-La Oroya highway. Zircon U-Pb age data from volcanic rocks and clastic basin fill provide a maximum depositional age of ~43 Ma for a middle Eocene syndeformational unit that we identify as the Anta Formation, which overlies the Paleocene Casapalca Formation. Sedimentary lithofacies and unconformable relationships within the volcaniclastic Anta Formation reveal mixed fluvial, alluvial-fan, and volcanic depositional conditions during shortening accommodated by a NE-verging thrust/reverse fault and corresponding backthrust (here named the Chonta fault system). Our cross-section reconstruction and geochronological data indicate that the region is a critical, possibly unique, zone of the broader NE-directed Marañon fold-thrust belt where pre-Neogene synorogenic sediments and their associated structures are preserved. We interpret this combined structural and basin system as an Eocene-age (Incaic) frontal thrust belt and corresponding foredeep to wedge-top depozone in central Peru. As one of the better-constrained segments of the Marañon fold-thrust belt, this zone provides insight into potential linkages with elusive early Cenozoic (Incaic) structures and foreland basin fill of the Western Cordillera and Altiplano farther south in the central Andean plateau.

  20. Validating a shortened depression scale (10 item CES-D among HIV-positive people in British Columbia, Canada.

    Directory of Open Access Journals (Sweden)

    Wendy Zhang

    Full Text Available OBJECTIVE: To establish the reliability and validity of a shortened (10-item depression scale used among HIV-positive patients enrolled in the Drug Treatment Program in British Columbia, Canada. METHODS: The 10-item CES-D (Center for Epidemiologic Studies Depression Scale was examined among 563 participants who initiated antiretroviral therapy (ART between August 1, 1996 and June 30, 2002. Internal consistency of the scale was measured by Cronbach's alpha. Using the original CES-D 20 as primary criteria, comparisons were made using the Kappa statistic. Predictive accuracy of CES-D 10 was assessed by calculating sensitivity, specificity, positive predictive values and negative predictive values. Factor analysis was also performed to determine if the CES-D 10 contained the same factors of positive and negative affect found in the original development of the CES-D. RESULTS: The correlation between the original and the shortened scale is very high (Spearman correlation coefficient  =0.97 (P<0.001. Internal consistency reliability coefficients of the CES-D 10 were satisfactory (Cronbach α=0.88. The CES-D 10 showed comparable accuracy to the original CES-D 20 in classifying participants with depressive symptoms (Kappa=0.82, P<0.001. Sensitivity of CES-D 10 was 91%; specificity was 92%; and positive predictive value was 92%. Factor analysis demonstrates that CES-D 10 contains the same underlying factors of positive and negative affect found in the original development of the CES-D 20. CONCLUSION: The 10-item CES-D is a comparable tool to measure depressive symptoms among HIV-positive research participants.

  1. LINE-1-derived poly(A) microsatellites undergo rapid shortening and create somatic and germline mosaicism in mice.

    Science.gov (United States)

    Grandi, Fiorella C; Rosser, James M; An, Wenfeng

    2013-03-01

    Interspersed and tandem repeat sequences comprise the bulk of mammalian genomes. Interspersed repeats result from successive replication by transposable elements, such as Alu and long interspersed element type 1 (L1). Microsatellites are tandem repeats of 1-6 base pairs, among which poly(A) microsatellites are the most abundant in the human genome. The rise and fall of a microsatellite has been depicted as a life cycle. Previous studies have demonstrated that Alu and L1 insertions are a major source of A-rich microsatellites owing to the concurrent formation of a poly(A) DNA tract at the 3'-end of each insertion. The fate of such poly(A) tracts has been studied by surveying the length distribution of genomic resident Alu and L1 insertions. However, these cross-sectional studies provide no information about the tempo of mutation immediately after birth. In this study, de novo L1 insertions were created using a transgenic L1 mouse model and traced through generations to investigate the early life of poly(A) microsatellites. High frequencies of intra-individual and intergenerational shortening were observed for long poly(A) tracts, creating somatic and germline mosaicism at the insertion site, whereas little variation was observed for short poly(A) alleles. As poly(A) microsatellites are the major intrinsic signal for nucleosome positioning, their remarkable abundance and variability make them a significant source of epigenetic variation. Thus, the birth of poly(A) microsatellites from retrotransposons and the subsequent rapid and variable shortening represent a new way with which retrotransposons can modify the genetic and epigenetic architecture of our genome.

  2. Differences in functional outcomes for adult patients with prosthodontically-treated and -untreated shortened dental arches: a systematic review.

    Directory of Open Access Journals (Sweden)

    Saadika Khan

    Full Text Available This review examined differences in functional outcomes and patient satisfaction when shortened dental arches are left untreated compared to their restoration to complete arch lengths with different prosthodontic interventions.A protocol was developed according to the criteria for a systematic review. All relevant databases were searched to identify appropriate clinical trials regardless of language or publication status. Predetermined eligibility criteria were applied, trial quality assessed and data extracted for each study. Relevant outcomes assessed were: functioning ability, patient satisfaction and harmful effects on oral structures.Searches yielded 101 articles: 81 from electronic databases and 20 from reference lists of retrieved articles (PEARLing searches. Sixty-nine citations were assessed for eligibility after removing 32 duplicate records. After reading titles and abstracts, a total of 41 records were excluded and the full-texts of the remaining 28 records were read. Only 21 records were included for the SR because 7 records were excluded after reading the full-text reports. These 21 records report the outcomes of four randomized controlled trials (RCTs and one non-randomized clinical trial (CT which were pre-specified and used for this review. No on-going studies were found and no eligible studies were excluded for failure to report the reviewer's pre-specified outcomes. Outcomes were reported in the retrieved 21 articles. A narrative explanation of the pre-specified outcomes is reported for the 3 comparison groups (which were based on the different interventions used for the individual clinical trials. The shortened dental arch as a treatment option is encouraging in terms of functioning, patient satisfaction and cost-effectiveness. By using only high quality studies it was expected that the results would be more reliable when making conclusions and recommendations, but some of the included studies had to be downgraded due to

  3. Calcite Twinning in the Ordovician Martinsburg Formation, Delaware Water Gap, New Jersey, USA: Implications for Cleavage Formation and Tectonic Shortening in the Appalachian Piedmont Province

    Directory of Open Access Journals (Sweden)

    John P. Craddock

    2016-02-01

    Full Text Available A traverse across the Stone Church syncline in the Ordovician Martinsburg turbidites reveals an axial planar cleavage (N40°E, SE dips in regional thrust-related folds (N40°E, shallow plunges and five phases of sparry calcite. Calcite fillings are bedding-parallel, cleavage-parallel, and one vein set cross-cuts both earlier phases; the youngest calcite filling is a bedding-parallel fault gouge that crosscuts the cleavage and preserves top-down-to-the-southeast normal fault kinematics. Calcite veins unique to disharmonically-folded calcareous siltstones (Maxwell, 1962 were also analyzed. Stable isotopic analysis (O, C of all of the calcite phases indicates a uniform fluid source (δ13C −2.0, δ18O −13.3 VPDB and, potentially, a similar precipitation and mechanical twinning age. The twinning strains (n = 1341; average Δσ = −32 MPa; average ε1 = −2.9% in the calcite suite are consistent with SE-NW thrust shortening, and sub-horizontal shortening perpendicular to evolving axial planar cleavage planes in the Stone Church syncline. Calcareous siltstone layers within the Martinsburg Fm. turbidites share concordant bedding planes and are unique, chemically (XRF, but folded and cleaved differently than the surrounding clay-rich Martinsburg turbidites. Neither sediment type yielded detrital zircons. Electron backscatter X-ray diffraction (EBSD and calcite twinning results in a folded calcareous siltstone layer preserving a layer-normal SE-NW shortening strain and Lattice Preferred Orientation (LPO. Shortening axes for the five-phase calcite suite trends ~N40°W, consistent with tectonic transport associated with crystalline nappe emplacement of the Reading Prong within the Piedmont province.

  4. Evaluating the superiority of honey over silver sulphadiazine dressing in shortening healing duration of burn injury: an evidence-based case report

    Directory of Open Access Journals (Sweden)

    Sandy S. Sopandi

    2013-12-01

    Full Text Available Background: Burn injury is a global health problem associated with major morbidity and mortality. Honey has long been used in wound management due to its ability to accelerate healing rates and prevent infection. This study is conducted to evaluate the efficacy of honey dressing in shortening healing duration of burn injury compared to silver sulphadiazine (SSD.Methods: A PubMed and Proquest database search was conducted to identify relevant studies. The studies were then appraised and ranked based on their validity, importance, and level of evidence.Results: The two studies appraised in this paper supported the supremacy of honey against SSD in shortening healing duration. However, the individual quality of studies involved was low.Conclusion: Honey is an alternative treatment in shortening burn wound healing duration. Further studies are needed to support clinical application of this conclusion. (Med J Indones. 2013;22:243-7. doi: 10.13181/mji.v22i4.608 Keywords: Burn injury, healing duration, honey, silver sulfadiazine

  5. Similar increases in muscle size and strength in young men after training with maximal shortening or lengthening contractions when matched for total work.

    Science.gov (United States)

    Moore, Daniel R; Young, Mark; Phillips, Stuart M

    2012-04-01

    Training exclusively with eccentric (lengthening) contractions can result in greater muscular adaptations than training with concentric (shortening) contractions. We aimed to determine whether training-induced increases in muscle size and strength differed between muscles performing maximal lengthening (LC) or maximal shortening (SC) contractions when total external work is equivalent. Nine healthy young males completed a 9-week isokinetic (0.79 rad/s) resistance training program of the elbow flexors whereby they performed LC with one arm and an equivalent volume of total external work with the contralateral arm as SC. Training increased isometric peak torque for both LC (~10%) and SC (~20%) with no difference (P = 0.14) between conditions. There were also similar increases in isokinetic peak torque at both slow (0.79 rad/s) and fast (5.24 rad/s) shortening and lengthening peak torque for both LC (~8-10%) and SC (~9-20%). Training increased work per repetition similarly for both LC (~17%) and SC (~22%), in spite of ~40% greater work per repetition with LC. The increase in muscle cross-sectional area with training was also similar (P = 0.37) between LC (~6.5%) and SC (~4.6%). We conclude that increases in muscle size and strength with short-term unilateral resistance training are unrelated to muscle contraction type when matched for both exercise intensity (i.e. maximal contractions) and total external work.

  6. Factor structure and psychometric properties of a French and German shortened version of the Behavioural Inhibition System/Behavioural Activation System scales.

    Science.gov (United States)

    Studer, Joseph; Baggio, Stéphanie; Mohler-Kuo, Meichun; Daeppen, Jean-Bernard; Gmel, Gerhard

    2016-03-01

    The Behavioural Inhibition System/Behavioural Activation System scales (BIS/BAS scales) constitute one of the most prominent questionnaires to assess individual differences in sensitivity to punishment and reward. However, some studies questioned its validity, especially that of the French and German translations. The aim of the present study was to re-evaluate the psychometric characteristics of the BIS/BAS scales in a large sample of French- and German-speaking young Swiss men (N = 5872). Results showed that factor structures previously found in the literature did not meet the standards of fit. Nine items had to be removed to achieve adequate fit statistics in confirmatory factor analysis, yielding a shortened version with four factors: one BIS factor comprising five items and three BAS factors, namely Reward Reactivity, Drive and Fun Seeking, each comprising two items. Convergent validity and group invariance analyses suggest that the shortened BIS/BAS scales constitute a valid and reliable instrument. Researchers interested in assessing individual differences in BIS and BAS reactivity in French- and German-speaking individuals should avoid using the BIS/BAS scales as originally specified. The shortened version may be a sound alternative at least in samples of young adults. Its shorter format may be particularly suited for surveys with constraints on questionnaire length.

  7. [Influence of cycle length shortening, atropine and beta-receptor blockage on sinus node recovery time (SRT) in patients with healthy sinus node (author's transl)].

    Science.gov (United States)

    Runge, M; Wohlberg, H; Luckmann, E; Pantlen, H

    1977-01-01

    Sinus node recovery time (SRT) was measured in 30 patients with healthy sinus nodes to examine the influence of the increase in atrial frequency, atropine and beta-receptor blockade. The measurements were performed following pacing with 3 atrial frequencies before and after administration of 1 mg atropine i.v. (13 patients) and 0.4 mg prindolol (Visken) i.v. (17 patients). Total group; Increase of frequency alone caused prolongation of the SRT in 17 patients and shortening in 13 patients. Atropine group: Blockade of the parasympathicus alone induced a highly significant reduction in the SRT. Simultaneous increase in frequency and blockade of the parasympathicus led to greater SRT-shortening during low frequency than with high frequency. This interrelationship of frequency and blockade of the parasympathicus influencing the SRT is statistically significant. Visken-group: Blockade of the sympathicus causes a highly significant prolongation of the SRT. Simultaneous increase in frequency and blockade of the sympathicus led to greater SRT-prolongation during low frequency than with high frequency. This interrelationship of frequency and blockade of the sympathicus influencing the SRT has slight statistical significance. The results are discussed in respect to the electrophysiological influences of atrial cycle length shortening, acetylcholine and catecholamines on the sinus node, the perinodal atrial fibres and the atrial working myocardium.

  8. New insight into the shortening of the collagen fibril D-period in human cornea.

    Science.gov (United States)

    Jastrzebska, Maria; Tarnawska, Dorota; Wrzalik, Roman; Chrobak, Artur; Grelowski, Michal; Wylegala, Edward; Zygadlo, Dorota; Ratuszna, Alicja

    2017-02-01

    Collagen fibrils type I display a typical banding pattern, so-called D-periodicity, of about 67 nm, when visualized by atomic force or electron microscopy imaging. Herein we report on a significant shortening of the D-period for human corneal collagen fibrils type I (21 ± 4 nm) upon air-drying, whereas no changes in the D-period were observed for human scleral collagen fibrils type I (64 ± 4 nm) measured under the same experimental conditions as the cornea. It was also found that for the corneal stroma fixed with glutaraldehyde and air-dried, the collagen fibrils show the commonly accepted D-period of 61 ± 8 nm. We used the atomic force microscopy method to image collagen fibrils type I present in the middle layers of human cornea and sclera. The water content in the cornea and sclera samples was varying in the range of .066-.085. Calculations of the D-period using the theoretical model of the fibril and the FFT approach allowed to reveal the possible molecular mechanism of the D-period shortening in the corneal collagen fibrils upon drying. It was found that both the decrease in the shift and the simultaneous reduction in the distance between tropocollagen molecules can be responsible for the experimentally observed effect. We also hypothesize that collagen type V, which co-assembles with collagen type I into heterotypic fibrils in cornea, could be involved in the observed shortening of the corneal D-period.

  9. Effect of oil and shortening in rice bread quality: Relationship between dough rheology and quality characteristics.

    Science.gov (United States)

    Mancebo, Camino M; Martínez, Mario M; Merino, Cristina; de la Hera, Esther; Gómez, Manuel

    2017-04-27

    One of the main problems with gluten-free breads is their texture and their rapid staling. Fats are widely used for the improvement of texture and other quality parameters in gluten-free breads. The effect of oil and shortening in rice-breads quality and its correlation with dough rheology has been analyzed. The inclusion of oil increased the specific volume of the breads and reduced their hardness, particularly with lower levels of hydration, whereas shortening did not modify specific volume or reduced it when hydration levels were higher. Oil, at levels of up to 30%, reduced the cohesiveness, springiness and resilience of breads, as well as the brightness of the crust, and increased the a* and b* values. Breads with oil also exhibited a greater number of pores per cm(2) , especially in doughs with higher levels of hydration. An inverse correlation between G' and G'' and bread specific volume has been observed, being the reciprocal-Y model a better predictor than the linear model to relate the bread specific volume. This study showed that the type and quantity of fat added in rice based breads affect the bread quality in a different way. In general, it can be said that the incorporation of up to 20% oil improved rice based breads. Oil increased the specific volume, the a* and b* parameters of the crust and the cell density. It also decreases hardness, cohesiveness, springiness, resilience, and the L* parameter. Converse to breads made with oil, the addition of shortening can negatively affect the quality of the breads. Moreover, the correlation analysis has demonstrated that the study of dough rheology could be a good predictor of gluten-free bread quality. © 2017 Wiley Periodicals, Inc.

  10. Telomere longitudinal shortening as a biomarker for dementia status of adults with Down syndrome.

    Science.gov (United States)

    Jenkins, Edmund C; Ye, Lingling; Krinsky-McHale, Sharon J; Zigman, Warren B; Schupf, Nicole; Silverman, Wayne P

    2016-03-01

    Previous studies have suggested that Alzheimer's disease (AD) causes an accelerated shortening of telomeres, the ends of chromosomes consisting of highly conserved TTAGGG repeats that, because of unidirectional 5'-3' DNA synthesis, lose end point material with each cell division. Our own previous work suggested that telomere length of T-lymphocytes might be a remarkably accurate biomarker for "mild cognitive impairment" in adults with Down syndrome (MCI-DS), a population at dramatically high risk for AD. To verify that the progression of cognitive and functional losses due to AD produced this observed telomere shortening, we have now examined sequential changes in telomere length in five individuals with Down syndrome (3F, 2M) as they transitioned from preclinical AD to MCI-DS (N = 4) or dementia (N = 1). As in our previous studies, we used PNA (peptide nucleic acid) probes for telomeres and the chromosome 2 centromere (as an "internal standard" expected to be unaffected by aging or dementia status), with samples from the same individuals now collected prior to and following development of MCI-DS or dementia. Consistent shortening of telomere length was observed over time. Further comparisons with our previous cross-sectional findings indicated that telomere lengths prior to clinical decline were similar to those of other adults with Down syndrome (DS) who have not experienced clinical decline while telomere lengths following transition to MCI-DS or dementia in the current study were comparable to those of other adults with DS who have developed MCI-DS or dementia. Taken together, findings indicate that telomere length has significant promise as a biomarker of clinical progression of AD for adults with DS, and further longitudinal studies of a larger sample of individuals with DS are clearly warranted to validate these findings and determine if and how factors affecting AD risk also influence these measures of telomere length.

  11. Cost and cost-effectiveness of tuberculosis treatment shortening: a model-based analysis.

    Science.gov (United States)

    Gomez, G B; Dowdy, D W; Bastos, M L; Zwerling, A; Sweeney, S; Foster, N; Trajman, A; Islam, M A; Kapiga, S; Sinanovic, E; Knight, G M; White, R G; Wells, W A; Cobelens, F G; Vassall, A

    2016-12-01

    Despite improvements in treatment success rates for tuberculosis (TB), current six-month regimen duration remains a challenge for many National TB Programmes, health systems, and patients. There is increasing investment in the development of shortened regimens with a number of candidates in phase 3 trials. We developed an individual-based decision analytic model to assess the cost-effectiveness of a hypothetical four-month regimen for first-line treatment of TB, assuming non-inferiority to current regimens of six-month duration. The model was populated using extensive, empirically-collected data to estimate the economic impact on both health systems and patients of regimen shortening for first-line TB treatment in South Africa, Brazil, Bangladesh, and Tanzania. We explicitly considered 'real world' constraints such as sub-optimal guideline adherence. From a societal perspective, a shortened regimen, priced at USD1 per day, could be a cost-saving option in South Africa, Brazil, and Tanzania, but would not be cost-effective in Bangladesh when compared to one gross domestic product (GDP) per capita. Incorporating 'real world' constraints reduces cost-effectiveness. Patient-incurred costs could be reduced in all settings. From a health service perspective, increased drug costs need to be balanced against decreased delivery costs. The new regimen would remain a cost-effective option, when compared to each countries' GDP per capita, even if new drugs cost up to USD7.5 and USD53.8 per day in South Africa and Brazil; this threshold was above USD1 in Tanzania and under USD1 in Bangladesh. Reducing the duration of first-line TB treatment has the potential for substantial economic gains from a patient perspective. The potential economic gains for health services may also be important, but will be context-specific and dependent on the appropriate pricing of any new regimen.

  12. Incorporated fish oil fatty acids prevent action potential shortening induced by circulating fish oil fatty acids

    Directory of Open Access Journals (Sweden)

    Hester M Den Ruijter

    2010-11-01

    Full Text Available Increased consumption of fatty fish, rich in omega-3 polyunsaturated fatty acids (3-PUFAs reduces the severity and number of arrhythmias. Long term 3-PUFA-intake modulates the activity of several cardiac ion channels leading to cardiac action potential shortening. Circulating 3-PUFAs in the bloodstream and incorporated 3-PUFAs in the cardiac membrane have a different mechanism to shorten the action potential. It is, however, unknown whether circulating 3-PUFAs in the bloodstream enhance or diminish the effects of incorporated 3-PUFAs. In the present study, we address this issue. Rabbits were fed a diet rich in fish oil (3 or sunflower oil (9, as control for 3 weeks. Ventricular myocytes were isolated by enzymatic dissociation and action potentials were measured using the perforated patch clamp technique in the absence and presence of acutely administered 3-PUFAs. Plasma of 3 fed rabbits contained more free eicosapentaenoic acid (EPA and isolated myocytes of 3 fed rabbits contained higher amounts of both EPA and docosahexaenoic acid (DHA in their sarcolemma compared to control. In the absence of acutely administered fatty acids, 3 myocytes had a shorter action potential with a more negative plateau than 9 myocytes. In the 9 myocytes, but not in the 3 myocytes, acute administration of a mixture of EPA+DHA shortened the action potential significantly. From these data we conclude that incorporated 3-PUFAs into the sarcolemma and acutely administered 3 fatty acids do not have a cumulative effect on action potential duration and morphology. As a consequence, patients with a high cardiac 3-PUFA status will probably not benefit from short term 3 supplementation as an antiarrhythmic therapy.

  13. Human probing behavior of Aedes aegypti when infected with a life-shortening strain of Wolbachia.

    Directory of Open Access Journals (Sweden)

    Luciano A Moreira

    Full Text Available BACKGROUND: Mosquitoes are vectors of many serious pathogens in tropical and sub-tropical countries. Current control strategies almost entirely rely upon insecticides, which increasingly face the problems of high cost, increasing mosquito resistance and negative effects on non-target organisms. Alternative strategies include the proposed use of inherited life-shortening agents, such as the Wolbachia bacterium. By shortening mosquito vector lifespan, Wolbachia could potentially reduce the vectorial capacity of mosquito populations. We have recently been able to stably transinfect Aedes aegypti mosquitoes with the life-shortening Wolbachia strain wMelPop, and are assessing various aspects of its interaction with the mosquito host to determine its likely impact on pathogen transmission as well as its potential ability to invade A. aegypti populations. METHODOLOGY/PRINCIPAL FINDINGS: Here we have examined the probing behavior of Wolbachia-infected mosquitoes in an attempt to understand both the broader impact of Wolbachia infection on mosquito biology and, in particular, vectorial capacity. The probing behavior of wMelPop-infected mosquitoes at four adult ages was examined and compared to uninfected controls during video-recorded feeding trials on a human hand. Wolbachia-positive insects, from 15 days of age, showed a drastic increase in the time spent pre-probing and probing relative to uninfected controls. Two other important features for blood feeding, saliva volume and apyrase content of saliva, were also studied. CONCLUSIONS/SIGNIFICANCE: As A. aegypti infected with wMelPop age, they show increasing difficulty in completing the process of blood feeding effectively and efficiently. Wolbachia-infected mosquitoes on average produced smaller volumes of saliva that still contained the same amount of apyrase activity as uninfected mosquitoes. These effects on blood feeding behavior may reduce vectorial capacity and point to underlying physiological

  14. [The probiotic yogurt Activia shortens intestinal transit, but has not been shown to promote defecation].

    Science.gov (United States)

    Katan, M B

    2008-03-29

    Activia is a yogurt product containing the probiotic bacterium Bifidobacterium animalis DN-173 010. Five clinical trials have been carried out. Four of these show that dairy products containing this bacterium shorten intestinal transit in volunteers. However, except in a subgroup of 19 out of 267 patients in one study, no significant effect of Activia was reported on the frequency, quantity or consistency of stools. In its marketing in the Netherlands, the company that produces Activia, Danone, claims that Activia promotes defecation. There is insufficient scientific evidence to support this claim.

  15. Shortened constraint-induced movement therapy in subacute stroke - no effect of using a restraint

    DEFF Research Database (Denmark)

    Brogårdh, Christina; Vestling, Monika; Sjölund, Bengt H

    2009-01-01

    OBJECTIVE: To examine the effect of using a mitt during shortened constraint-induced movement therapy for patients in the subacute phase after stroke. SUBJECTS: Twenty-four patients with stroke (mean age 57.6 (standard deviation (SD) 8.5) years; average 7 weeks post-stroke) with mild to moderate......, no statistically significant differences between the groups were found in any measures at any point in time. CONCLUSION: In this study, no effect of using a restraint in patients with subacute stroke was found. Thus, this component in the constraint-induced therapy concept seems to be of minor importance...

  16. Single clear corneal incision for glaucoma drainage device shortening in pediatric glaucoma.

    Science.gov (United States)

    Radke, Phillip M; Bitrian, Elena; Grajewski, Alana L

    2016-06-01

    Glaucoma drainage devices are commonly used for management of glaucoma in adults and children. With time, the position of the tube can change and cause damage such as corneal scarring, iris or lens contact, and uveitis. Most of these problems can be improved with tube shortening and/or excision of adherent iris or fibrous tissue. We describe a surgical technique that uses a single clear corneal incision to externalize and trim the shunt in pediatric patients. The technique has a short surgical. We review the indications and outcomes for this procedure in 13 eyes of 12 children who required shunt revision.

  17. [Carotid endarterectomy. Experiences with shortening of interval between symptom and operation

    DEFF Research Database (Denmark)

    Rathenborg, L.K.; Baekgaard, N.; Jensen, Leif Pandora

    2008-01-01

    and in the neurological departments. The stroke and death rate was unchanged, 4% and 3% before and after fast track respectively. CONCLUSION: The time between symptom and CEA can be shortened by means of a fast track after thorough information and reorganization of the work involving these patients. In order to bring......INTRODUCTION: Carotid endarterectomy (CEA) prevents transient ischemic attack and stroke in patients with symptomatic high-grade carotid stenosis. In 2004 Rothwell et al showed that maximal benefit is gained if CEA is performed less than three weeks after the onset of the symptom. With the aim...

  18. Depth oriented brief therapy: an ideal technique as hospice lengths-of-stay continue to shorten.

    Science.gov (United States)

    Thomson, Judith E; Jordan, Merle R

    2002-01-01

    The authors note that as hospice patients' lengths-of-stay continue to shorten, psychosocial/spiritual counselors are being challenged to help patients and families process the myriad of issues terminal illness gives rise to. Given this reality, the authors suggest that the Depth Oriented Brief Therapy (DOBT) approach should prove especially useful. The DOBT premise is that if people can be helped to experience the emotional meanings of why they hold on to emotionally painful symptoms then they can abandon their symptoms for healthier ways of being.

  19. Physicochemical properties of shortening base oil rich in medium-and long-chain triacylglycerols%富含中长链甘三酯起酥油基料油的理化性质研究

    Institute of Scientific and Technical Information of China (English)

    张阳; 杨博; 王永华

    2014-01-01

    The nutritional and functional shortening base oils with low calorie, zero-trans fatty acid were prepared by palm stearin( PS) and medium- and long-chain triacylglycerols( MLCT) blended with differ-ent volume ratios (2:8, 3:7, 4:6, 5:5, 6:4, 7:3),and the physicochemical properties of the six kinds of shortening base oils were studied. The results showed that there were no trans fatty acid detected in the shortening base oils, and the linoleic acid contents were 18% -36%;the solid fat content ( SFC) of the shortening base oil increased with PS content increasing, and the solid fat content of the shortening base oil with 30% of PS met the requirement of ideal cake shortening;the content ofβ′crystal in the shorten-ing base oil increased with PS content increasing;theβcrystal converted toβ′crystal when the shortening base oil was endothermic melted, and the calorimetry curve was closely related to the compositions of fatty acids and triglycerides.%将棕榈硬脂(PS)和中长碳链甘三酯(MLCT)以6种不同体积比(2:8、3:7、4:6、5:5、6:4、7:3)混合来制备低热量、零反式脂肪酸的营养功能性起酥油基料油,并对6种起酥油基料油的理化性质进行研究。结果表明:6种起酥油基料油中不含反式脂肪酸,亚油酸含量在18%~36%;随着PS含量增加,起酥油基料油的固体脂肪含量增加,含有30%PS的起酥油基料油的固体脂肪含量符合理想蛋糕用起酥油要求;随着PS含量的增加,起酥油基料油的β′晶型含量增加;起酥油基料油吸热熔化的过程中存在β晶型向β′晶型转变的过程,其量热曲线与脂肪酸组成和甘三酯组成密切相关。

  20. Two-stage revision of infected hip arthroplasty using a shortened post-operative course of antibiotics.

    LENUS (Irish Health Repository)

    McKenna, Paul B

    2009-04-01

    We present a series of 30 consecutive patients with 31 infected total hip arthroplasties treated by a single surgeon over a 4-year period in whom a shortened post-operative course of antimicrobial chemotherapy was used.

  1. Effects of removable dental prostheses on masticatory performance of subjects with shortened dental arches: A systematic review

    NARCIS (Netherlands)

    Liang, S.; Zhang, Q.; Witter, D.J.; Wang, Y.; Creugers, N.H.J.

    2015-01-01

    OBJECTIVE: To synthesise data on the effects of distal-extension removable dental prostheses (RDPs) on masticatory performance of subjects with (extreme) shortened dental arches ((E)SDAs). DATA: Search terms were: 'masticatory' respectively 'chewing' combined with 'performance', 'efficiency', or

  2. In-treatment midwall and endocardial fractional shortening predict cardiovascular outcome in hypertensive patients with preserved baseline systolic ventricular function: the Losartan Intervention For Endpoint reduction study

    DEFF Research Database (Denmark)

    Wachtell, Kristian; Gerdts, Eva; Palmieri, Vittorio;

    2010-01-01

    Endocardial fractional shortening (EFS) and midwall shortening (MWS) are impaired in patients with left ventricular hypertrophy. However, it remains unknown whether improvement of left ventricular systolic function during treatment reduces cardiovascular morbidity and mortality in hypertensive pa...

  3. Variability in New Shortening Estimates from Southern Peru (12-14S); Implications for Mass Balance of the Andean Plateau.

    Science.gov (United States)

    Gotberg, N.; McQuarrie, N.

    2008-12-01

    One of the fundamental questions of interest with regards to the Andean Plateau is the mass balance of material needed to create and sustain a 3-4 km high plateau. Is crustal shortening sufficient to support an isostatically compensated crust of 60-70km? We present new estimates of shortening across the northern margin of the Andean Plateau. The cross section extent, from the eastern edge of the volcanic arc to foreland basin, is approximately one half of the physiographic width of the Andean Plateau in Peru. Cross sectional shortening estimates in southern Peru (12-14°S) provide a best estimate of 123 km or 40% shortening with an absolute minimum estimate of 86 km or 30% and absolute maximum estimate of 275 km or 60%. We determined the maximum and minimum shortening estimates using the cross sectional area and possible variations in assumptions made about the amount of erosion, detachment dip, involvement of basement thrusts and displacement along faults. The best estimate of shortening is well short of the required 240-300km of shortening needed in order to account for a 60-70km thick crust under the entire plateau. This suggests that for an isostatically equilibrated crust either 1) there is a significant amount of shortening (~150km) in the western half of the plateau which, is hidden by the volcanic arc or 2) crustal material is being added to the Peruvian section of the Andean Plateau either through lower crustal flow or a process of magmatic underplating followed by differentiation and delamination.

  4. Changes in the maximum speed of shortening of frog muscle fibres early in a tetanic contraction and during relaxation.

    Science.gov (United States)

    Josephson, R K; Edman, K A

    1998-03-01

    1. Isotonic shortening velocities at very light loads were examined in single fibres of the anterior tibialis muscle of the frog, Rana temporaria, using load-clamp recording and slack tests (temperature, 1-3 degrees C; initial sarcomere length, 2.25 microns). 2. Shortening velocities at very light loads (force-clamp recording) were found to be higher early in the rise of a tetanic contraction than during the plateau of the contraction. The upper limit of the load at which there was elevated shortening velocity early in the contraction was 1.5-5.4% of the maximum tetanic tension (Fo) depending on the particular fibre. 3. The maximum shortening velocity determined using the slack test method (Vo) was as much as 30% greater early in a contraction than at the tetanic plateau. Vo was elevated above the plateau level up to about 30 ms after the end of the latent period, which is equivalent to the time required for the force in an isometric contraction to rise to about 30% of Fo. Vo is depressed below the plateau value during relaxation at the cessation of stimulation. 4. Stimulation studies show that the cross-bridge model of Huxley (1957) predicts the maximum shortening velocity to be greater early in a contraction, when new actin binding sites are becoming activated and new cross-bridge connections are being formed rapidly, than during steady-state contraction. The elevated shortening velocity in the model is a consequence of new cross-bridges being formed in the pulling configuration, and there being a delay before the newly added bridges are dragged beyond their equilibrium position so they begin to retard shortening. The model also predicts that maximum shortening velocity should be depressed below the plateau level during early relaxation as cross-bridge binding sites are rapidly removed from the active population.

  5. Myosin heavy chain isoform expression regulates shortening velocity in smooth muscle: studies using an SMB KO mouse line.

    Science.gov (United States)

    Karagiannis, Peter; Babu, G J; Periasamy, M; Brozovich, Frank V

    2004-01-01

    The kinetics of smooth muscle are thought to be partially determined by the level of the expression of the 7 amino acid insert, SMB, in the myosin heavy chain, as SMB is generally expressed at higher levels in faster smooth muscle. In this study, we determined the role of this insert on shortening velocity and force regeneration following rapid reduction in muscle length (k(step)) in bladder tissue from a transgenic mouse line expressing the insert at three different levels: wild type (WT, +/+, SMB/SMB), an SMA homozygous type (SMB KO, -/-), and a heterozygous type (+/-, SMB/SMA). Smooth muscle from +/+ bladder shorten faster than both the +/- and -/- bladder smooth muscle when activated with Ca2+, consistent with SMB determining the shortening velocity of smooth muscle. The addition of Pi to the fully activated skinned bladder strips did not affect the rate of shortening for either the +/+ or -/- bladder types but did significantly decrease the rate of shortening for the +/- type. In contrast, the addition of ADP to fully Ca2+ activated bladder strips increased the rate of shortening for all three bladder types. However after thiophosphorylation, ADP slowed the shortening velocity. These data are consistent with shortening velocity being determined by the level of activation (or crossbridge attachment) in smooth muscle. The rates of force regeneration according to the k(step) protocol showed no differences between bladder types and also proved insensitive to either Pi or ADP. These data suggest that the rates of force regeneration were determined not only by the kinetics of the crossbridge cycle, but also by factors outside the contractile apparatus.

  6. Comparison of hydrogenated vegetable shortening and nutritionally complete high fat diet on limited access-binge behavior in rats

    OpenAIRE

    Davis, Jon F.; Melhorn, Susan J; Heiman, Justin U.; Tschöp, Matthias H.; Clegg, Deborah J.; Benoit, Stephen C.

    2007-01-01

    Previous studies have suggested that intermittent exposure to hydrogenated vegetable shortening yields a binge/compensate pattern of feeding in rats. The present study was designed to assess whether rats would exhibit similar patterns of intake when given intermittent access to a nutritionally complete high-fat diet. Four groups of rats received varying exposure to either hydrogenated vegetable shortening or high-fat diet for 8 consecutive weeks. Animals were given daily and intermittent acce...

  7. Mechanical versus kinematical shortening reconstructions of the Zagros High Folded Zone (Kurdistan region of Iraq)

    Science.gov (United States)

    Frehner, Marcel; Reif, Daniel; Grasemann, Bernhard

    2012-06-01

    This paper compares kinematical and mechanical techniques for the palinspastic reconstruction of folded cross sections in collision orogens. The studied area and the reconstructed NE-SW trending, 55.5 km long cross section is located in the High Folded Zone of the Zagros fold-and-thrust belt in the Kurdistan region of Iraq. The present-day geometry of the cross section has been constructed from field as well as remote sensing data. In a first step, the structures and the stratigraphy are simplified and summarized in eight units trying to identify the main geometric and mechanical parameters. In a second step, the shortening is kinematically estimated using the dip domain method to 11%-15%. Then the same cross section is used in a numerical finite element model to perform dynamical unfolding simulations taking various rheological parameters into account. The main factor allowing for an efficient dynamic unfolding is the presence of interfacial slip conditions between the mechanically strong units. Other factors, such as Newtonian versus power law viscous rheology or the presence of a basement, affect the numerical simulations much less strongly. If interfacial slip is accounted for, fold amplitudes are reduced efficiently during the dynamical unfolding simulations, while welded layer interfaces lead to unrealistic shortening estimates. It is suggested that interfacial slip and decoupling of the deformation along detachment horizons is an important mechanical parameter that controlled the folding processes in the Zagros High Folded Zone.

  8. Increasing flux rate to shorten leaching period and ramp-up production

    Science.gov (United States)

    Ngantung, Billy; Agustin, Riska; Ravi'i

    2017-01-01

    J Resources Bolaang Mongondow (JBRM) has operated a dynamic heap leach in its Bakan Gold Mine since late 2013. After successfully surpassing its name plate capacity of 2.6 MT/annum in 2014, the clayey and transition ore become the next operational challenge. The presence of transition and clayey ore requires longer leaching period, hence reducing the leach pad capacity which then caused reduced production. Maintaining or even increasing production with such longer leaching ore types can be done by expanding the leach pad area which means an additional capital investment, and/or shortening the leaching cycle which compromise a portion of gold extraction. JBRM has been successfully increasing the leach pad production from 2.6 MT/annum to 3.8 MT/annum, whilst improving the gold extraction from around 70% to around 80%. This was achieved by managing the operation of the leach pad which is shortening the leach cycle by identifying and combining the optimal flux rate application versus the tonne processed in each cell, at no capital investment for expanding the cell capacity.

  9. Analysis of modulation factor to shorten the delivery time in helical tomotherapy.

    Science.gov (United States)

    Shimizu, Hidetoshi; Sasaki, Koji; Tachibana, Hiroyuki; Tomita, Natsuo; Makita, Chiyoko; Nakashima, Kuniyasu; Yokoi, Kazushi; Kubota, Takashi; Yoshimoto, Manabu; Iwata, Tohru; Kodaira, Takeshi

    2017-05-01

    A low modulation factor (MF) maintaining a good dose distribution contributes to the shortening of the delivery time and efficiency of the treatment plan in helical tomotherapy. The purpose of this study was to reduce the delivery time using initial values and the upper limit values of MF. First, patients with head and neck cancer (293 cases) or prostate cancer (181 cases) treated between June 2011 and July 2015 were included in the analysis of MF values. The initial MF value (MFinitial ) was defined as the average MFactual value, and the upper limit of the MF value (MFUL ) was defined according the following equation: MFUL = 2 × standard deviation of MFactual value + the average MFactual Next, a treatment plan was designed for patients with head and neck cancer (62 cases) and prostate cancer (13 cases) treated between December 2015 and June 2016. The average MFactual value for the nasopharynx, oropharynx, hypopharynx, and prostate cases decreased from 2.1 to 1.9 (p = 0.0006), 1.9 to 1.6 (p delivery time for the nasopharynx, oropharynx, hypopharynx, and prostate cases also decreased from 19.9 s cm(-1) to 16.7 s cm(-1) (p delivery time was shortened by the adaptation of MFinitial and MFUL values with a reduction in the average MFactual for head and neck cancer and prostate cancer cases. © 2017 The Authors. Journal of Applied Clinical Medical Physics published by Wiley Periodicals, Inc. on behalf of American Association of Physicists in Medicine.

  10. Diffusional kurtosis MRI of the lower leg: changes caused by passive muscle elongation and shortening.

    Science.gov (United States)

    Filli, Lukas; Kenkel, David; Wurnig, Moritz C; Boss, Andreas

    2016-06-01

    Diffusional kurtosis MRI (DKI) quantifies the deviation of water diffusion from a Gaussian distribution. We investigated the influence of passive elongation and shortening of the lower leg muscles on the DKI parameters D (diffusion coefficient) and K (kurtosis). After approval by the local ethics committee, eight healthy volunteers (age, 29.1 ± 2.9 years) underwent MRI of the lower leg at 3 T. Diffusion-weighted images were acquired with 10 different b values at three ankle positions (passive dorsiflexion 10°, neutral position 0°, passive plantar flexion 40°). Parametrical maps of D and K were obtained by voxel-wise fitting of the signal intensities using a non-linear Levenberg-Marquardt algorithm. D and K were measured in the tibialis anterior, medial and lateral gastrocnemius, and soleus muscles. In the neutral position, D and K values were in the range between 1.66-1.79 × 10(-3) mm(2) /s and 0.21-0.39, respectively. D and K increased with passive shortening, and decreased with passive elongation, which could also be illustrated on the parametrical maps. In dorsiflexion, D (p muscles are significantly influenced by the ankle joint position, indicating that the diffusion of water molecules in skeletal muscle deviates from a Gaussian distribution depending on muscle tonus. Copyright © 2016 John Wiley & Sons, Ltd.

  11. Measurement of terrace deformation and crustal shortening of some renascent fold zones within Kalpin nappe structure

    Institute of Scientific and Technical Information of China (English)

    YANG XiaoPing; RAN YongKang; CHENG JianWu; CHEN LiChun; XU XiWei

    2007-01-01

    The Kalpin nappe structure is a strongest thrust and fold deformation belt in front of the Tianshan Mountains since the Cenozoic time. The tectonic deformation occurred in 5-6 striking Mesozoic-Cenozoic fold zones, and some renascent folds formed on the recent alluvial-proluvial fans in front of the folded mountains. We used the total station to measure gully terraces along the longitudinal topographic profile in the renascent fold zones and collected samples from terrace deposits for age determination. Using the obtained formation time and shortening amount of the deformed terraces, we calculated the shortening rate of 4 renascent folds to be 0.1±0.03 mm/a, 0.12±0.04 mm/a, 0.59±0.18 mm/a, and 0.26±0.08 mm/a, respectively. The formation time of the renascent folds is some later than the major tectonic uplift event of the Qinghai-Tibet Plateau 0.14 Ma ago. It may be the long-distance effect of this tectonic event on the Tianshan piedmont fold belt.

  12. Animal studies of life shortening and cancer risk from space radiation

    Science.gov (United States)

    Wood, D. H.; Yochmowitz, M. G.; Hardy, K. A.; Salmon, Y. L.

    The U. S. Air Force study of the delayed effects of single, total body exposures to simulated space radiation in rhesus monkeys is now in its 21st year. Observations on 301 irradiated and 57 age-matched control animals indicate that life expectancy loss from exposure to protons in the energy range encountered in the Van Allen belts and solar proton events can be expressed as a logarithmic function of the dose. The primary causes of life shortening are cancer and endometriosis (an abnormal proliferation of the lining of the uterus in females). Life shortening estimates permit comparison of the risk associated with space radiation exposures to be compared with that of other occupational and environmental hazards, thereby facilitating risk/benefit decisions in the planning and operational phases of manned space missions. Calculations of the relative risk of fatal cancers in the irradiated subjects reveal that the total body surface dose required to double the risk of death from cancer over a 20-year post exposure period varies with the linear energy transfer (LET) of the radiation. The ability to determine the integrated dose and LET spectrum in space radiation exposures of human is, therefore, critical to the assessment of life-time cancer risk.

  13. Leucocyte Telomere Shortening in relation to Newly Diagnosed Type 2 Diabetic Patients with Depression

    Directory of Open Access Journals (Sweden)

    Zhelong Liu

    2014-01-01

    Full Text Available The goal of this study is to investigate the association between oxidative stress and telomere length shortening in the comorbid depression and diabetes. Therefore, 71 patients with newly diagnosed type 2 diabetes (T2D and 52 subjects with normal glycemic level (control, Ctrl were enrolled. Depressive status was identified with the Depression Subscale of Hospital Anxiety and Depression Scale (HADS-D. Leukocyte telomere length ratio (T/S ratio was determined with quantitative PCR. Oxidative stress status was evaluated with 8-hydroxy-desoxyguanosine (8-OHdG assay kit. Some other biochemical blood testing was also performed. The data showed that T2D patients had higher proportion of depression evaluated by the HADS-D (x2=4.196, P=0.041. T/S ratio was significantly negatively correlated with 8-OHdG, HADS-D, age, HbA1c, FPG, and HOMA-IR. In addition, HADS-D was significantly positively correlated with HbA1c, FPG, HOMA-IR, and 8-OHdG. Both HADS-D and 8-OHdG were the major independent predictors for T/S ratio. This study indicates that oxidative stress contributes to both telomere length shortening and depression development in newly diagnosed type 2 diabetic patients, while in depression status, some other mechanisms besides oxidative stress may also affect the telomere length.

  14. An analysis of the temperature dependence of force, during steady shortening at different velocities, in (mammalian) fast muscle fibres.

    Science.gov (United States)

    Roots, H; Ranatunga, K W

    2008-01-01

    We examined, over a wide range of temperatures (10-35 degrees C), the isometric tension and tension during ramp shortening at different velocities (0.2-4 L(0)/s) in tetanized intact fibre bundles from a rat fast (flexor hallucis brevis) muscle; fibre length (L(0)) was 2.2 mm and sarcomere length approximately 2.5 microm. During a ramp shortening, the tension change showed an initial inflection of small amplitude (P(1)), followed by a larger exponential decline towards an approximate steady level; the tension continued to decline slowly afterwards and the approximate steady tension at a given velocity was estimated as the tension (P(2)) at the point of intersection between two linear slopes, as previously described (Roots et al. 2007). At a given temperature, the tension P(2) declined to a lower level and at a faster rate (from an exponential curve fit) as the shortening velocity was increased; the temperature sensitivity of the rate of tension decline during ramp shortening at different velocities was low (Q(10) 0.9-1.5). The isometric tension and the P(2) tension at a given shortening velocity increased with warming so that the relation between tension and (reciprocal) temperature was sigmoidal in both. In isometric muscle, the temperature T(0.5) for half-maximal tension was approximately 10 degrees C, activation enthalpy change (DeltaH) was approximately 100 kJ mol(-1) and entropy change (DeltaS) approximately 350 J mol(-1) K(-1). In shortening, these were increased with increase of velocity so that at a shortening velocity (approximately 4 L(0)/s) producing maximal power at 35 degrees C, T(0.5) was approximately 28 degrees C, DeltaH was approximately 200 kJ mol(-1) and DeltaS approximately 700 J mol(-1) K(-1); the same trends were seen in the tension data from isotonic release experiments on intact muscle and in ramp shortening experiments on maximally Ca-activated skinned fibres. In general, our findings show that the sigmoidal relation between force and

  15. Effect of positional changes of anatomic structures on upper airway dilating muscle shortening during electro- and chemostimulation.

    Science.gov (United States)

    Oliven, A; Odeh, M

    2006-09-01

    Positional changes of anatomic structures surrounding the upper airway are known to affect pharyngeal mechanics and collapsibility. We hypothesized that these alterations also affect the ability of the upper airway dilator muscles to enlarge the pharynx by altering their ability to shorten when activated. Using sonomicrometry, we evaluated in seven anesthetized dogs the effects of changes in tracheal and head position on the length of the genioglossus (GG) and the geniohyoid (GH) and the effects of these positional changes on the magnitude of shortening of the two muscles in response to electro- (ES) and chemostimulation (CS). Caudal traction of the trachea lengthened the GG and GH in all dogs, whereas cranial displacement of the trachea and flexion of the head to a vertical position shortened the muscles. Compared with the magnitude of ES-induced shortening in the neutral position, ES-induced shortening of the GG was 144.7 +/- 14.6, 49.3 +/- 4.3, and 33.5 +/- 11.6% during caudal and cranial displacement of the trachea and during head flexion, respectively. Similar effects of the positional changes were found for the GH, as well as for both muscles during respiratory stimulation with P(CO2) of 90 Torr at the end of CO(2) rebreathing, although inspiratory muscle shortening during CS reached only one-quarter to one-third of the magnitude observed during ES. We conclude that positional alterations of anatomic structures in the neck have a dramatic effect on the magnitude of shortening of the activated GG and GH, which may reduce substantially their ability to protect pharyngeal patency.

  16. Characterization of graininess formed in all beef tallow-based shortening.

    Science.gov (United States)

    Meng, Zong; Liu, Yuan-Fa; Jin, Qing-Zhe; Huang, Jian-Hua; Song, Zhi-Hua; Wang, Feng-Yan; Wang, Xing-Guo

    2010-11-10

    A batch of all beef tallow (BT)-based model shortening (divided into six rectangular block samples) was stored under temperature fluctuation cycles of 5-20 °C until granular crystals were observed. The lipid composition, thermal properties, and polymorphism of the granular crystals and their surrounding materials were evaluated. Furthermore, the isothermal crystallization behavior of two parts noted above was also examined by pulsed nuclear magnetic resonance (pNMR), rheology, and polarized light microscopy (PLM). The changes of nanostructure including the aggregation of high-melting triacylglycerols (TAGs) and transformation into the most stable β polymorph occurred in granular crystals compared with surrounding materials. Concomitantly, a slower crystallization rate with a simultaneous increase in crystal growth led to the formation of large crystals and further aggregated to larger granular crystals when the size ultimately exceeded the sensory threshold.

  17. To the problem of cross-bridge tension in steady muscle shortening and lengthening

    CERN Document Server

    Kokshenev, Valery B

    2009-01-01

    Despite the great success of the Huxley sliding filament model proposed half a century ago for actin-myosin linkages (cross-bridges), it fails to explain the force-velocity behavior of stretching skeletal muscles. Huxley's two-state kinetic equation for cross-bridge proportions is therefore reconsidered and a new solution to the problem of steady muscle eccentric and concentric contractions is reported. When the second law of statistical thermodynamics is applied to cross-bridge proportions, the weakly bound states appear to be correlated to the strongly bound states via structural and kinetic intrinsic muscle characteristics. The explicit force-velocity curve fits the empirical tension-velocity data on frog muscle shortening using only one adjustable parameter, while the Huxley model employed four parameters.

  18. Shortening trinucleotide repeats using highly specific endonucleases: a possible approach to gene therapy?

    Science.gov (United States)

    Richard, Guy-Franck

    2015-04-01

    Trinucleotide repeat expansions are involved in more than two dozen neurological and developmental disorders. Conventional therapeutic approaches aimed at regulating the expression level of affected genes, which rely on drugs, oligonucleotides, and/or transgenes, have met with only limited success so far. An alternative approach is to shorten repeats to non-pathological lengths using highly specific nucleases. Here, I review early experiments using meganucleases, zinc-finger nucleases (ZFN), and transcription-activator like effector nucleases (TALENs) to contract trinucleotide repeats, and discuss the possibility of using CRISPR-Cas nucleases to the same end. Although this is a nascent field, I explore the possibility of designing nucleases and effectively delivering them in the context of gene therapy.

  19. Effect of shortening kraft pulping integrated with extended oxygen delignification on biorefinery process performance of eucalyptus.

    Science.gov (United States)

    Li, Jing; Zhang, Chunyun; Hu, Huichao; Chai, Xin-Sheng

    2016-02-01

    The aim of this work was to study the impact of shortening kraft pulping (KP) process integrated with extended oxygen delignification (OD) on the biorefinery process performance of eucalyptus. Data showed that using kraft pulps with high kappa number could improve the delignification efficiency of OD, reduce hexenuronic acid formation in kraft pulps. Pulp viscosity for a target kappa number of ∼10 was comparable to that obtained from conventional KP and OD process. The energy and alkali consumption in the integrated biorefinery process could be optimized when using a KP pulp with kappa number of ∼27. The process could minimize the overall methanol formation, but greater amounts of carbonate and oxalate were formed. The information from this study will be helpful to the future implementation of short-time KP integrated with extended OD process in actual pulp mill applications for biorefinery, aiming at further improvement in the biorefinery effectiveness of hardwood. Copyright © 2015 Elsevier Ltd. All rights reserved.

  20. A Posteriori Equivalence: A New Perspective for Design of Optimal Channel Shortening Equalizers

    CERN Document Server

    Venkataramani, Raman

    2007-01-01

    The problem of channel shortening equalization for optimal detection in ISI channels is considered. The problem is to choose a linear equalizer and a partial response target filter such that the combination produces the best detection performance. Instead of using the traditional approach of MMSE equalization, we directly seek all equalizer and target pairs that yield optimal detection performance in terms of the sequence or symbol error rate. This leads to a new notion of a posteriori equivalence between the equalized and target channels with a simple characterization in terms of their underlying probability distributions. Using this characterization we show the surprising existence an infinite family of equalizer and target pairs for which any maximum a posteriori (MAP) based detector designed for the target channel is simultaneously MAP optimal for the equalized channel. For channels whose input symbols have equal energy, such as q-PSK, the MMSE equalizer designed with a monic target constraint yields a so...

  1. Age-dependent Muscle Adaptation after Chronic Stretch-shortening Contractions in Rats.

    Science.gov (United States)

    Rader, Erik P; Layner, KaylaN; Triscuit, Alyssa M; Chetlin, Robert D; Ensey, James; Baker, Brent A

    2016-01-01

    Age-related differences in contraction-induced adaptation have been well characterized especially for young and old rodent models but much less so at intermediate ages. Therefore, additional research is warranted to determine to what extent alterations in adaptation are due to maturation versus aging per se. The purpose of our study was to evaluate muscles of Fisher 344XBrown Norway rats of various ages following one month of exposure to stretch-shortening contractions (SSCs). With exposure, muscles mass increased by ~10% for 27 and 30 month old rats vs. ~20% for 3 and 6 month old rats (P muscle performance in general beginning at late adulthood. Such findings motivate careful investigation to determine appropriate SSC exposures at all stages of life.

  2. The Paley ulnarization of the carpus with ulnar shortening osteotomy for treatment of radial club hand

    Directory of Open Access Journals (Sweden)

    Paley Dror

    2017-01-01

    Full Text Available Recurrent deformity from centralization and radialization led to the development in 1999 of a new technique by the author called ulnarization. This method is performed through a volar approach in a vascular and physeal sparing fashion. It biomechanically balances the muscle forces on the wrist by dorsally transferring the flexor carpi ulnaris (FCU from a deforming to a corrective force. The previous problems of a prominent bump from the ulnar head and ulnar deviation instability were solved by acutely shortening the diaphysis and by temporarily fixing the station of the carpus to the ulnar head at the level of the scaphoid. This is the first report of this modified Paley ulnarization method, which the author considers a significant improvement over his original procedure.

  3. The Paley ulnarization of the carpus with ulnar shortening osteotomy for treatment of radial club hand

    Science.gov (United States)

    Paley, Dror

    2017-01-01

    Recurrent deformity from centralization and radialization led to the development in 1999 of a new technique by the author called ulnarization. This method is performed through a volar approach in a vascular and physeal sparing fashion. It biomechanically balances the muscle forces on the wrist by dorsally transferring the flexor carpi ulnaris (FCU) from a deforming to a corrective force. The previous problems of a prominent bump from the ulnar head and ulnar deviation instability were solved by acutely shortening the diaphysis and by temporarily fixing the station of the carpus to the ulnar head at the level of the scaphoid. This is the first report of this modified Paley ulnarization method, which the author considers a significant improvement over his original procedure. PMID:28120747

  4. Present-day shortening in Southern Haiti from GPS measurements and implications for seismic hazard

    Science.gov (United States)

    Symithe, Steeve; Calais, Eric

    2016-06-01

    The ~ 3 M inhabitant capital region of Haiti, severely affected by the devastating January 12, 2010, M7.0 earthquake, continues to expand at a fast rate. Accurate characterization of regional earthquake sources is key to inform urban development and construction practices through improved regional seismic hazard estimates. Here we use a recently updated Global Positioning System (GPS) data set to show that seismogenic strain accumulation in southern Haiti involves an overlooked component of shortening on a south-dipping reverse fault along the southern edge of the Cul-de-Sac basin, in addition to the well-known component of left-lateral strike-slip motion. This tectonic model implies that ground shaking may be twice that expected if the major fault was purely strike-slip, as assumed in the current seismic hazard map for the region.

  5. Summary update of the Brookhaven tritium toxicity program with emphasis on recent cytogenetic and lifetime-shortening studies

    Energy Technology Data Exchange (ETDEWEB)

    Carsten, A.L.; Benz, R.D.; Hughes, W.P.; Ichimasa, Yusuke; Ikushima, Takaji; Tezuka, Hideo

    1988-01-01

    A number of years ago a multiparameter program to evaluate the toxicity of tritiated water (HTO) was undertaken in the Medical Department at Brookhaven National Laboratory. The results of most of these studies have been published and will receive brief attention. Emphasis will be placed on the unpublished studies involving the induction of sister chromatid exchanges (SCE's) in bone marrow of mice, new biochemical information, and preliminary results on lifetime-shortening and carcinogenesis. In brief, male Hale-Stoner Brookhaven (HSB) mice maintained on HTO concentrations ranging from 3.0 to 30.0 ..mu..Ci/ml exhibited essentially the same number of SCE's per cell throughout their lifetime. Control mice showed a decrease in number of SCE's with age. The lack of a dose-response effect and the constant level of SCE's in HTO mice as compared to controls will be discussed. In the carcinogenesis study C57BL/6J male mice received various x-ray or HTO regimens. Mortality data from these and other studies in which CBA/Ca/BNL mice received single x-ray exposures or equivalent integrated dose exposures by single HTO injections will be discussed. 25 refs., 4 figs.

  6. Estrogen deficiency leads to telomerase inhibition,telomere shortening and reduced cell proliferation in the adrenal gland of mice

    Institute of Scientific and Technical Information of China (English)

    Sharyn Bayne; Margaret EE Jones; He Li; Alex R Pinto; Evan R Simpson; Jun-Ping Liu

    2008-01-01

    Estrogen deficiency mediates aging, but the underlying mechanism remains to be fully determined. We report here that estrogen deficiency caused by targeted disruption of aromatase in mice results in significant inhibition oftelomerase activity in the adrenal gland in vivo. Gene expression analysis showed that, in the absence of estrogen, telomerase reverse transcriptase (TERT) gene expression is reduced in association with compromised cell proliferation in the adrenal gland cortex and adrenal atrophy. Stem cells positive in c-kit are identified to populate in the parenchyma of adrenal cortex. Analysis of telomeres revealed that estrogen deficiency results in significantly shorter telomeres in the adrenal cortex than that in wild-type (WT) control mice. To further establish the causal effects of estrogen, we conducted an estrogen replacement therapy in these estrogen-deficient animals. Administration of estrogen for 3 weeks restores TERT gene expression, telomerase activity and cell proliferation in estrogen-deficient mice. Thus, our data show for the first time that estrogen deficiency causes inhibitions of TERT gene expression, telomerase activity, telomere maintenance, and cell proliferation in the adrenal gland of mice in vivo, suggesting that telomerase inhibition and telomere shortening may mediate cell proliferation arrest in the adrenal gland, thus contributing to estrogen deficiency-induced aging under physiological conditions.

  7. Shortening rates across the foothills of the Western Kunlun (Xinjiang, China) inferred from geomorphic measurements and cosmogenic 10Be dating.

    Science.gov (United States)

    Coudroy, T.; van der Woerd, J.; Li, H.; Barrier, L.; Tapponnier, P.; Simoes, M.; Thuizat, R.; Pan, J.; Si, J.; Xu, T.

    2009-04-01

    -surface samples are similarly high (> 8.106 at/g/yr), indicative of steady-state, with minimum ages of 500 kyr, and a maximum deflation rate of 0.001 mm/yr. Given these values, the maximum incision rate into the lower of these two terraces would have been 0.14 mm/yr. These preliminary results imply minimum foreland shortening rates only on order or 1-2 mm/yr, far less than might be expected for a Cenozoic mountain range of such height and width. More dating and geomorphic measurements are presently in progress across other active structures.

  8. Foot lengthening and shortening during gait: a parameter to investigate foot function?

    Science.gov (United States)

    Stolwijk, N M; Koenraadt, K L M; Louwerens, J W K; Grim, D; Duysens, J; Keijsers, N L W

    2014-02-01

    Based on the windlass mechanism theory of Hicks, the medial longitudinal arch (MLA) flattens during weight bearing. Simultaneously, foot lengthening is expected. However, changes in foot length during gait and the influence of walking speed has not been investigated yet. The foot length and MLA angle of 34 healthy subjects (18 males, 16 females) at 3 velocities (preferred, low (preferred -0.4 m/s) and fast (preferred +0.4 m/s) speed were investigated with a 3D motion analysis system (VICON(®)). The MLA angle was calculated as the angle between the second metatarsal head, the navicular tuberculum and the heel in the local sagittal plane. Foot length was calculated as the distance between the marker at the heel and the 2nd metatarsal head. A General Linear Model for repeated measures was used to indicate significant differences in MLA angle and foot length between different walking speeds. The foot lengthened during the weight acceptance phase of gait and shortened during propulsion. With increased walking speed, the foot elongated less after heel strike and shortened more during push off. The MLA angle and foot length curve were similar, except between 50% and 80% of the stance phase in which the MLA increases whereas the foot length showed a slight decrease. Foot length seems to represent the Hicks mechanism in the foot and the ability of the foot to bear weight. At higher speeds, the foot becomes relatively stiffer, presumably to act as a lever arm to provide extra propulsion. Copyright © 2013 Elsevier B.V. All rights reserved.

  9. Grafted vertebral fracture after implant removal in a patient with spine-shortening vertebral osteotomy.

    Science.gov (United States)

    Nakashima, Hiroaki; Yukawa, Yasutsugu; Ito, Keigo; Machino, Masaaki; Kanbara, Shunsuke; Morita, Daigo; Imagama, Shiro; Ishiguro, Naoki; Kato, Fumihiko

    2015-02-01

    We experienced the rare complication of a vertebral fracture that was caused by implant removal after bony fusion had been achieved in a patient who underwent spine-shortening osteotomy (SSO) for tethered cord syndrome (TCS). We propose that the removal of the implant used for SSO should be contraindicated. The patient (a 27-year-old female) presented to our institution with a history of progressive severe lower back pain, gait disturbance, and urinary incontinence. As an infant, she had undergone surgery for spina bifida with lipoma. Magnetic resonance imaging of the spine revealed tethering of the spinal cord to a lipoma. We performed SSO at the level of the L1 vertebra level. After spine shortening and fixation using a posterior approach, the L1 vertebral body was completely removed anteriorly and replaced with a left iliac bone graft. The patient's symptoms improved after surgery. After bony fusion was achieved after surgery, we decided to remove the spinal implant after we explained the advantages and disadvantages of the procedure to the patient. We performed implant removal surgery safely 2 years later; however, the patient complained of severe lower back pain 10 days after the surgery without any history of trauma. Reconstruction computed tomography showed fracture of the grafted vertebra. We performed a repeat posterior fixation, which relieved the lower back pain; she has experienced no complications in the subsequent 5 years. In summary, we report a case of a rare complication of the fracture of a grafted vertebra after removal of an implant used in SSO for TCS. Spinal stability could not be maintained without the spinal posterior implant after SSO. Postoperative fracture after spinal implant removal is rare but possible, and patients must be informed of this potential risk.

  10. Anesthetic Sevoflurane Causes Rho-Dependent Filopodial Shortening in Mouse Neurons.

    Directory of Open Access Journals (Sweden)

    Jeffrey H Zimering

    Full Text Available Early postnatal anesthesia causes long-lasting learning and memory impairment in rodents, however, evidence for a specific neurotoxic effect on early synaptogenesis has not been demonstrated. Drebrin A is an actin binding protein whose localization in dendritic protrusions serves an important role in dendritic spine morphogenesis, and is a marker for early synaptogenesis. We therefore set out to investigate whether clinically-relevant concentrations of anesthetic sevoflurane, widely- used in infants and children, alters dendritic morphology in cultured fetal day 16 mouse hippocampal neurons. After 7 days in vitro, mouse hippocampal neurons were exposed to four hours of 3% sevoflurane in 95% air/5% CO2 or control condition (95% air/5% CO2. Neurons were fixed in 4% paraformaldehyde and stained with Alexa Fluor555-Phalloidin, and/or rabbit anti-mouse drebrin A/E antibodies which permitted subcellular localization of filamentous (F-actin and/or drebrin immunoreactivity, respectively. Sevoflurane caused acute significant length-shortening in filopodia and thin dendritic spines in days-in-vitro 7 neurons, an effect which was completely rescued by co-incubating neurons with ten micromolar concentrations of the selective Rho kinase inhibitor Y27632. Filopodia and thin spine recovered in length two days after sevoflurane exposure. Yet cluster-type filopodia (a precursor to synaptic filopodia were persistently significantly decreased in number on day-in-vitro 9, in part owing to preferential localization of drebrin immunoreactivity to dendritic shafts versus filopodial stalks. These data suggest that sevoflurane induces F-actin depolymerization leading to acute, reversible length-shortening in dendritic protrusions through a mechanism involving (in part activation of RhoA/Rho kinase signaling and impairs localization of drebrin A to filopodia required for early excitatory synapse formation.

  11. Shortening Delivery Times of Intensity Modulated Proton Therapy by Reducing Proton Energy Layers During Treatment Plan Optimization

    Energy Technology Data Exchange (ETDEWEB)

    Water, Steven van de, E-mail: s.vandewater@erasmusmc.nl [Department of Radiation Oncology, Erasmus MC Cancer Institute, Rotterdam (Netherlands); Kooy, Hanne M. [F. H. Burr Proton Therapy Center, Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts (United States); Heijmen, Ben J.M.; Hoogeman, Mischa S. [Department of Radiation Oncology, Erasmus MC Cancer Institute, Rotterdam (Netherlands)

    2015-06-01

    Purpose: To shorten delivery times of intensity modulated proton therapy by reducing the number of energy layers in the treatment plan. Methods and Materials: We have developed an energy layer reduction method, which was implemented into our in-house-developed multicriteria treatment planning system “Erasmus-iCycle.” The method consisted of 2 components: (1) minimizing the logarithm of the total spot weight per energy layer; and (2) iteratively excluding low-weighted energy layers. The method was benchmarked by comparing a robust “time-efficient plan” (with energy layer reduction) with a robust “standard clinical plan” (without energy layer reduction) for 5 oropharyngeal cases and 5 prostate cases. Both plans of each patient had equal robust plan quality, because the worst-case dose parameters of the standard clinical plan were used as dose constraints for the time-efficient plan. Worst-case robust optimization was performed, accounting for setup errors of 3 mm and range errors of 3% + 1 mm. We evaluated the number of energy layers and the expected delivery time per fraction, assuming 30 seconds per beam direction, 10 ms per spot, and 400 Giga-protons per minute. The energy switching time was varied from 0.1 to 5 seconds. Results: The number of energy layers was on average reduced by 45% (range, 30%-56%) for the oropharyngeal cases and by 28% (range, 25%-32%) for the prostate cases. When assuming 1, 2, or 5 seconds energy switching time, the average delivery time was shortened from 3.9 to 3.0 minutes (25%), 6.0 to 4.2 minutes (32%), or 12.3 to 7.7 minutes (38%) for the oropharyngeal cases, and from 3.4 to 2.9 minutes (16%), 5.2 to 4.2 minutes (20%), or 10.6 to 8.0 minutes (24%) for the prostate cases. Conclusions: Delivery times of intensity modulated proton therapy can be reduced substantially without compromising robust plan quality. Shorter delivery times are likely to reduce treatment uncertainties and costs.

  12. Pre-power-stroke cross-bridges contribute to force transients during imposed shortening in isolated muscle fibers.

    Directory of Open Access Journals (Sweden)

    Fabio C Minozzo

    Full Text Available When skeletal muscles are activated and mechanically shortened, the force that is produced by the muscle fibers decreases in two phases, marked by two changes in slope (P₁ and P₂ that happen at specific lengths (L₁ and L₂. We tested the hypothesis that these force transients are determined by the amount of myosin cross-bridges attached to actin and by changes in cross-bridge strain due to a changing fraction of cross-bridges in the pre-power-stroke state. Three separate experiments were performed, using skinned muscle fibers that were isolated and subsequently (i activated at different Ca²⁺ concentrations (pCa²⁺ 4.5, 5.0, 5.5, 6.0 (n = 13, (ii activated in the presence of blebbistatin (n = 16, and (iii activated in the presence of blebbistatin at varying velocities (n = 5. In all experiments, a ramp shortening was imposed (amplitude 10%L₀, velocity 1 L₀•sarcomere length (SL•s⁻¹, from an initial SL of 2.5 µm (except by the third group, in which velocities ranged from 0.125 to 2.0 L₀•s⁻¹. The values of P₁, P₂, L₁, and L₂ did not change with Ca²⁺ concentrations. Blebbistatin decreased P₁, and it did not alter P₂, L₁, and L₂. We developed a mathematical cross-bridge model comprising a load-dependent power-stroke transition and a pre-power-stroke cross-bridge state. The P₁ and P₂ critical points as well as the critical lengths L₁ and L₂ were explained qualitatively by the model, and the effects of blebbistatin inhibition on P₁ were also predicted. Furthermore, the results of the model suggest that the mechanism by which blebbistatin inhibits force is by interfering with the closing of the myosin upper binding cleft, biasing cross-bridges into a pre-power-stroke state.

  13. Pre-power-stroke cross-bridges contribute to force transients during imposed shortening in isolated muscle fibers.

    Science.gov (United States)

    Minozzo, Fabio C; Hilbert, Lennart; Rassier, Dilson E

    2012-01-01

    When skeletal muscles are activated and mechanically shortened, the force that is produced by the muscle fibers decreases in two phases, marked by two changes in slope (P₁ and P₂) that happen at specific lengths (L₁ and L₂). We tested the hypothesis that these force transients are determined by the amount of myosin cross-bridges attached to actin and by changes in cross-bridge strain due to a changing fraction of cross-bridges in the pre-power-stroke state. Three separate experiments were performed, using skinned muscle fibers that were isolated and subsequently (i) activated at different Ca²⁺ concentrations (pCa²⁺ 4.5, 5.0, 5.5, 6.0) (n = 13), (ii) activated in the presence of blebbistatin (n = 16), and (iii) activated in the presence of blebbistatin at varying velocities (n = 5). In all experiments, a ramp shortening was imposed (amplitude 10%L₀, velocity 1 L₀•sarcomere length (SL)•s⁻¹), from an initial SL of 2.5 µm (except by the third group, in which velocities ranged from 0.125 to 2.0 L₀•s⁻¹). The values of P₁, P₂, L₁, and L₂ did not change with Ca²⁺ concentrations. Blebbistatin decreased P₁, and it did not alter P₂, L₁, and L₂. We developed a mathematical cross-bridge model comprising a load-dependent power-stroke transition and a pre-power-stroke cross-bridge state. The P₁ and P₂ critical points as well as the critical lengths L₁ and L₂ were explained qualitatively by the model, and the effects of blebbistatin inhibition on P₁ were also predicted. Furthermore, the results of the model suggest that the mechanism by which blebbistatin inhibits force is by interfering with the closing of the myosin upper binding cleft, biasing cross-bridges into a pre-power-stroke state.

  14. Assessment of range of motion and muscular shortening in female flamenco dancers. Valoración de las amplitudes articulares y acortamientos musculares en bailaoras de flamenco.

    OpenAIRE

    José L. Costa Sepúlveda; Jorge del R. Fernández Santos; Roque Gómez Espinosa de los Monteros; Ana González Galo

    2011-01-01

    The purpose of the study was to assess flexibility and range of motion in flamenco dancers of Cádiz, Sevilla and Jaén (Spain) through a tests battery. The study population comprised 37 healthy flamenco dancers (25 ± 7,2 years, 1,6 ± 0,5 m y 56 ± 7,6 Kg). They performed a range of flexibility and motion tests (i.e. Kendall test, Nachlas test). Results has shown that there is muscle shortening in most of the tests that flamenco dancers has passed. We conclude that there are not many articles on...

  15. Neogene shortening and exhumation of the Zagros fold-thrust belt and foreland basin in the Kurdistan region of northern Iraq

    Science.gov (United States)

    Koshnaw, Renas I.; Horton, Brian K.; Stockli, Daniel F.; Barber, Douglas E.; Tamar-Agha, Mazin Y.; Kendall, Jerome J.

    2017-01-01

    The Zagros fold-thrust belt in the Kurdistan region of Iraq encroached southward toward a rapidly subsiding Neogene foreland basin and was later partitioned by out-of-sequence shortening focused along the Mountain Front Flexure (MFF), as defined by new low-temperature thermochronologic, stratigraphic, and provenance results. Apatite (U-Th)/He ages document rapid deformation advance from the Main Zagros Fault to southern frontal structures (Kirkuk, Shakal, and Qamar thrusts) at 10-8 Ma, followed by potential basement-involved out-of-sequence development of the MFF (Qaradagh anticline) by 5 Ma. Distinct shifts in detrital zircon U-Pb provenance signatures for Neogene foreland basin fill provide evidence for drainage reorganization during fold-thrust belt advance. U-Pb age spectra and petrologic data from the Injana (Upper Fars) Formation indicate derivation from a variety of Eurasian, Pan-African, ophiolitic and Mesozoic-Cenozoic volcanic terranes, whereas the Mukdadiya (Lower Bakhtiari) and Bai-Hasan (Upper Bakhtiari) Formations show nearly exclusive derivation from the Paleogene Walash-Naopurdan volcanic complex near the Iraq-Iran border. Such a sharp cutoff in Eurasian, Pan-African, and ophiolitic sources is likely associated with drainage reorganization and tectonic development of the geomorphic barrier formed by the MFF. As a result of Zagros crustal shortening, thickening and loading, the Neogene foreland basin developed and accommodated an abrupt influx of fluvial clastic sediment that contains growth stratal evidence of synkinematic accumulation. The apparent out-of-sequence pattern of upper crustal shortening in the hinterland to foreland zone of Iraqi Kurdistan suggests that structural inheritance and the effects of synorogenic erosion and accumulation are important factors influencing the irregular and episodic nature of orogenic growth in the Zagros.

  16. Modulation of soleus H-reflex during shortening and lengthening muscle actions in young and older adults.

    Science.gov (United States)

    Chen, Yung-Sheng; Zhou, Shi; Cartwright, Colleen

    2015-02-28

    The H-reflex is dependently modulated during isometric and anisometric muscle actions. However, the manner of the H-reflex modulation during dynamic muscle movements in relation to ageing is less stated in the literature. This study was designed to investigate the effects of ageing on soleus (SOL) H-reflex modulation during dynamic muscle actions. Twenty young (24 ± 4 years of age) and 20 older adults (73 ± 5 years of age) voluntarily participated in the study. The SOL H-reflex was measured during passive and active shortening and lengthening muscle actions in a sitting position. The older group showed a lower ratio of the maximal amplitude of H-reflex to M-wave (SOL Hmax/Mmax) during the passive lengthening than that during the passive shortening (shortening: 0.40 ± 0.22 vs. lengthening: 0.15 ± 0.10, P shortening than that during the lengthening contractions at maximal effort (shortening: 0.51 ± 0.26 vs. lengthening: 0.37 ± 0.18, P muscle actions between young and older adults.

  17. Tension and heat production during isometric contractions and shortening in the anterior byssus retractor muscle of Mytilus edulis.

    Science.gov (United States)

    Gilbert, S H

    1978-09-01

    1. Tension and heat production were measured during phasic isometric contractions and isovelocity shortening in the anterior byssus retractor muscle (ABRM) of Mytilus edulis at 20 degrees C. 2. Isometric tension at lo was 550 +/- 40 mN/mm2 (S.D. for 173 observations in nine muscles), while the isometric maintenance heat rate was 1.0 +/- 0.2 mW/g wet wt. (S.D. for seventy-eight observations in eight muscles). 3. Isometric tension and heat production were measured as functions of muscle length over a range of 0.79--1.14 lo and were found to bear a linear relation to each other. 4. The force-velocity relation was determined in isovelocity releases imposed during tetanic stimulation and was found to fit the Hill equation with parameters alpha/Po = 0.07 +/- 0.01 and b/lo = 0.016 +/- 0.0007 sec-1 (S.E. from non-linear least-squares regression of the pooled data from seven experiments). 5. Heat production measured in the same experiments showed that shortening heat is produced with a shortening heat coefficient alpha/Po of 0.15. Shortening heat does not appear to be force-dependent, and separate experiments confirmed that it is a linear function of the amount of shortening.

  18. The maximum velocity of shortening during the early phases of the contraction in frog single muscle fibres.

    Science.gov (United States)

    Lombardi, V; Menchetti, G

    1984-10-01

    The maximum velocity of shortening (Vmax) was determined at preset times during the development and the plateau of isometric tetani in single fibres isolated from the tibialis anterior muscle of the frog. Experiments were performed at low temperature (3.6-6 degrees C) and at about 2.25 micron sarcomere length. The controlled velocity release method was used. Vmax was measured by determining the lowest velocity of release required to keep the tension at zero. Extreme care was taken in dissection and mounting of the fibres in order to make the passive series compliance very small. The value of Vmax at the end of the latent period for the development of isometric tension (at 4.5 degrees C about 10 ms after the beginning of the stimulus volley) was already the same as later during either the tension rise or at the plateau of isometric tetani. These results show that the value of Vmax of intact fibres is independent of time and activation subsequent to the latent period, and suggest that the cycling rate of the crossbridges may thus attain its steady-state value just at the end of the isometric latent period.

  19. Evidence for shortening the duration of clinical trials of antidepressants and a proposed paradigm for such studies.

    Science.gov (United States)

    Katz, Martin M; Berman, Nancy; Bowden, Charles L; Frazer, Alan

    2015-06-01

    The model for the clinical trial of putative antidepressants is older than 50 years. Recent failures resulted in several drug companies, citing excessive costs of lengthy multiweek trials, abandoning new drug development. Collateral problems include patients being maintained on ineffective drugs for 6 to 8 weeks, increasing the pain associated with the disorder. This study proposes an alternative model for testing new drugs that both shortens the clinical trial and broadens its aims to include a profile of the new drug's specific clinical actions. This alternative model makes it possible to uncover the drug's application to treatment of other mental disorders. It is based on recent findings that onset of action and a large proportion of an effective drug's positive effects, contrary to early reports, occur within the first 2 weeks. It uses an index of the 2-week "early improvement" to predict a 6-week outcome. Measuring effects on the dimensions of the disorder determined that effective antidepressants act on mood and behavioral components and that the Hamilton and new "multivantaged" methods can provide a profile of specific drug actions distinguished from nonspecific placebo effects, at 2 weeks. This early improvement is predictive of positive outcome of 6-week trials. Because of the implications of successful 2-week trials for reducing costs, providing data on specific clinical drug actions, potentially stimulating new drug development, and reducing patient suffering from extended treatment with ineffective drugs, a large sample, prospective study designed in accord with this test trial is recommended.

  20. Stretch-shortening cycle muscle power in women and men aged 18-81 years: Influence of age and gender.

    Science.gov (United States)

    Edwén, C E; Thorlund, J B; Magnusson, S P; Slinde, F; Svantesson, U; Hulthén, L; Aagaard, P

    2014-08-01

    This study explored the age-related deterioration in stretch-shortening cycle (SSC) muscle power and concurrent force-velocity properties in women and men across the adult life span. A total of 315 participants (women: n = 188; men: n = 127) aged 18-81 years performed maximal countermovement jumps on an instrumented force plate. Maximal SSC leg extension power expressed per kg body mass (Ppeak) was greater in men than in women across the adult age span (P < 0.001); however, this gender difference was progressively reduced with increasing age, because men showed an ∼50% faster rate of decline in SSC power than women (P < 0.001). Velocity at peak power (VPpeak) was greater in men than in women (P < 0.001) but declined at a greater rate in men than in women (P = 0.002). Vertical ground reaction force at peak power (FPpeak) was higher in men than in women in younger adults only (P < 0.001) and the age-related decline was steeper in men than in women (P < 0.001). Men demonstrated a steeper rate of decline in Ppeak than women with progressive aging. This novel finding emerged as a result of greater age-related losses in men for both force and velocity. Consequently, maximal SSC power production was observed to converge between genders when approaching old age.

  1. Fast characterization of line-end shortening and application of novel correction algorithms in e-beam direct write

    Science.gov (United States)

    Freitag, Martin; Choi, Kang-Hoon; Gutsch, Manuela; Hohle, Christoph; Galler, Reinhard; Krüger, Michael; Weidenmueller, Ulf

    2011-04-01

    For the manufacturing of semiconductor technologies following the ITRS roadmap, we will face the nodes well below 32nm half pitch in the next 2~3 years. Despite being able to achieve the required resolution, which is now possible with electron beam direct write variable shaped beam (EBDW VSB) equipment and resists, it becomes critical to precisely reproduce dense line space patterns onto a wafer. This exposed pattern must meet the targets from the layout in both dimensions (horizontally and vertically). For instance, the end of a line must be printed in its entire length to allow a later placed contact to be able to land on it. Up to now, the control of printed patterns such as line ends is achieved by a proximity effect correction (PEC) which is mostly based on a dose modulation. This investigation of the line end shortening (LES) includes multiple novel approaches, also containing an additional geometrical correction, to push the limits of the available data preparation algorithms and the measurement. The designed LES test patterns, which aim to characterize the status of LES in a quick and easy way, were exposed and measured at Fraunhofer Center Nanoelectronic Technologies (CNT) using its state of the art electron beam direct writer and CD-SEM. Simulation and exposure results with the novel LES correction algorithms applied to the test pattern and a large production like pattern in the range of our target CDs in dense line space features smaller than 40nm will be shown.

  2. Exercise-induced expression of cardiac ATP-sensitive potassium channels promotes action potential shortening and energy conservation

    Science.gov (United States)

    Zingman, Leonid V.; Zhu, Zhiyong; Sierra, Ana; Stepniak, Elizabeth; Burnett, Colin M-L.; Maksymov, Gennadiy; Anderson, Mark E.; Coetzee, William A.; Hodgson-Zingman, Denice M.

    2011-01-01

    Physical activity is one of the most important determinants of cardiac function. The ability of the heart to increase delivery of oxygen and metabolic fuels relies on an array of adaptive responses necessary to match bodily demand while avoiding exhaustion of cardiac resources. The ATP-sensitive potassium (KATP) channel has the unique ability to adjust cardiac membrane excitability in accordance with ATP and ADP levels, and up-regulation of its expression that occurs in response to exercise could represent a critical element of this adaption. However, the mechanism by which KATP channel expression changes result in a beneficial effect on cardiac excitability and function remains to be established. Here, we demonstrate that an exercise-induced rise in KATP channel expression enhanced the rate and magnitude of action potential shortening in response to heart rate acceleration. This adaptation in membrane excitability promoted significant reduction in cardiac energy consumption under escalating workloads. Genetic disruption of normal KATP channel pore function abolished the exercise-related changes in action potential duration adjustment and caused increased cardiac energy consumption. Thus, an expression-driven enhancement in the KATP channel-dependent membrane response to alterations in cardiac workload represents a previously unrecognized mechanism for adaptation to physical activity and a potential target for cardioprotection. PMID:21439969

  3. Effects of a static stretch using a load on low back pain patients with shortened tensor fascia lata.

    Science.gov (United States)

    Bae, Hae-In; Kim, Dae-Young; Sung, Yun-Hee

    2017-04-01

    Stretch of tensor fascia lata (TFL) improves range of motion on hip and pelvis and it reported to help reduce low back pain. Accordingly, the purpose of this study was to investigate effects of static stretching using a load on TFL in patients with low back pain. Twenty three subjects were recruited according to the selection criteria. The subjects were randomly assigned to static stretching group (control, n=12), and a static stretching using a load group (experimental, n=11). All group performed stretching for 15 min (side for 50 sec per time and a rest for 30 sec) per day in the left side and the right, respectively, for 2 weeks. Before and after the intervention, all groups measured visual analogue scale (VAS), stand and reach test, and the Oswestry disability index (ODI). In the present results, we found that the experimental group showed significant differences in VAS, stand and reach test, and the ODI (Pstatic stretching using a load can be actively utilized for low back pain patients with shortened TFL.

  4. Atrazine and malathion shorten the maturation process of Xenopus laevis oocytes and have an adverse effect on early embryo development.

    Science.gov (United States)

    Ji, Qichao; Lee, Jessica; Lin, Yu-Huey; Jing, Guihua; Tsai, L Jillianne; Chen, Andrew; Hetrick, Lindsay; Jocoy, Dylan; Liu, Junjun

    2016-04-01

    The use of pesticides has a negative impact on the environment. Amphibians have long been regarded as indicator species to pollutants due to their permeable skin and sensitivity to the environment. Studies have shown that population declines of some amphibians are directly linked with exposure to agricultural contaminants. In the past, much of the studies have focused on the toxic effect of contaminants on larvae (tadpoles), juvenile and adult frogs. However, due to the nature of their life cycle, amphibian eggs and early embryos are especially susceptible to the contaminants, and any alteration during the early reproductive stages may have a profound effect on the health and population of amphibians. In this study, we analyzed the effect of atrazine and malathion, two commonly used pesticides, on Xenopus laevis oocyte maturation and early embryogenesis. We found that both atrazine and malathion shortened the frog oocyte maturation process and resulted in reduced Emi2 levels at cytostatic factor-mediated metaphase arrest, and a high level of Emi2 is critically important for oocyte maturation. Furthermore, frog embryos fertilized under the influence of atrazine and/or malathion displayed a higher rate of abnormal division that eventually led to embryo death during early embryogenesis.

  5. Shortening spinal column reconstruction through posterior only approach for the treatment of unstable osteoporotic burst lumber fracture: a case report.

    Science.gov (United States)

    Shawky, Ahmed; Kroeber, Markus

    2013-02-01

    Case report. This study reports if shortening reconstruction procedure through posterior approach only can be used in osteoporotic unstable fracture as well as post-traumatic burst fracture. An 80-year-old female patient with unstable burst osteoporotic fracture of L1 underwent posterior approach corpectomy and shortening reconstruction of the spinal column by non-expandable cages. The surgery was uneventful, with average blood loss. Using of small profile cages has helped us to avoid root injury. Augmentation of the screw with cement and the compressive force applied to the spine column aids in obtaining a rigid construct with good alignment without any neurological complication. Shortening reconstruction procedure through only posterior approach is a viable option in treating unstable osteoporotic fracture as well as post-traumatic fractures. Using non-expandable cage is advocated to avoid cage subsidence.

  6. Parametric second Stokes Raman laser output pulse shortening to 300 ps due to depletion of pumping of intracavity Raman conversion

    Science.gov (United States)

    Smetanin, S. N.; Jelínek, M.; Kubeček, V.; Jelínková, H.; Ivleva, L. I.

    2016-10-01

    A new effect of the pulse shortening of the parametrically generated radiation down to hundreds of picosecond via depletion of pumping of intracavity Raman conversion in the miniature passively Q-switched Nd: SrMoO4 parametric self-Raman laser with the increasing energy of the shortened pulse under pulsed pumping by a high-power laser diode bar is demonstrated. The theoretical estimation of the depletion stage duration of the convertible fundamental laser radiation via intracavity Raman conversion is in agreement with the experimentally demonstrated duration of the parametrically generated pulse. Using the mathematical modeling of the pulse shortening quality and quantity deterioration is disclosed, and the solution ways are found by the optimization of the laser parameters.

  7. Astrocytes phagocytose focal dystrophies from shortening myelin segments in the optic nerve of Xenopus laevis at metamorphosis.

    Science.gov (United States)

    Mills, Elizabeth A; Davis, Chung-ha O; Bushong, Eric A; Boassa, Daniela; Kim, Keun-Young; Ellisman, Mark H; Marsh-Armstrong, Nicholas

    2015-08-18

    Oligodendrocytes can adapt to increases in axon diameter through the addition of membrane wraps to myelin segments. Here, we report that myelin segments can also decrease their length in response to optic nerve (ON) shortening during Xenopus laevis metamorphic remodeling. EM-based analyses revealed that myelin segment shortening is accomplished by focal myelin-axon detachments and protrusions from otherwise intact myelin segments. Astrocyte processes remove these focal myelin dystrophies using known phagocytic machinery, including the opsonin milk fat globule-EGF factor 8 (Mfge8) and the downstream effector ras-related C3 botulinum toxin substrate 1 (Rac1). By the end of metamorphic nerve shortening, one-quarter of all myelin in the ON is enwrapped or internalized by astrocytes. As opposed to the removal of degenerating myelin by macrophages, which is usually associated with axonal pathologies, astrocytes selectively remove large amounts of myelin without damaging axons during this developmental remodeling event.

  8. Treatment of Crowe Type-IV Hip Dysplasia Using Cementless Total Hip Arthroplasty and Double Chevron Subtrochanteric Shortening Osteotomy: A 5- to 10-Year Follow-Up Study.

    Science.gov (United States)

    Li, Xigong; Lu, Yang; Sun, Junying; Lin, Xiangjin; Tang, Tiansi

    2017-02-01

    The purpose of this study was to evaluate the functional and radiographic results of patients with Crowe type-IV hip dysplasia treated by cementless total hip arthroplasty and double chevron subtrochanteric osteotomy. From January 2000 to February 2006, cementless total hip arthroplasty with a double chevron subtrochanteric shortening osteotomy was performed on 18 patients (22 hips) with Crowe type-IV dysplasia. The acetabular cup was placed in the position of the anatomic hip center, and subtrochanteric femoral shortening osteotomy was performed with the use of a double chevron design. The clinical and radiographic outcomes were reviewed with a mean follow-up of 6.5 years (5-10 years). The mean amount of femoral subtrochanteric shortening was 38 mm (25-60 mm). All osteotomy sites were healed by 3-6 months without complications. The mean Harris Hip Score improved significantly from 47 points (35-65 points) preoperatively to 88 points (75-97 points) at the final follow-up. The Trendelenburg sign was corrected from a positive preoperative status to a negative postoperative status in 12 of 22 hips. No acetabular and femoral components have loosened or required revision during the period of follow-up. Cementless total hip arthroplasty using double chevron subtrochanteric osteotomy allowed for restoration of anatomic hip center with safely functional limb lengthening, achieved correction of preoperative limp, and good functional and radiographic outcomes for 22 Crowe type-IV dislocation hips at the time of the 5- to 10-year follow-up. Copyright © 2016 Elsevier Inc. All rights reserved.

  9. A Late Quaternary shortening rate for the frontal thrust of the Andean Precordillera north of Mendoza

    Science.gov (United States)

    Schmidt, S.; Kuhlmann, J.; Hetzel, R.; Mingorance, F.; Ramos, V. A.

    2009-12-01

    Although large historical earthquakes occurred in the Andean back-arc region between 28° and 34°S - for instance Mendoza was destroyed by an earthquake of magnitude MS = 7.0 in 1861 - the slip rates of active faults remain unknown. We report a slip rate for the 50-km-long Las Penas thrust, which constitutes the frontal thrust of the Precordillera. In its southern part, a well preserved fluvial terrace along La Escondida Creek (Costa et al., 2000) is displaced vertically by 10.6 ± 0.7 m as documented by several fault scarp profiles. Apart from radiocarbon dating of plant remnants, three different approaches for 10Be exposure dating have been applied to constrain the age of the terrace. Amalgamated sandstone pebbles (corrected for an inherited 10Be component using similar pebbles from the active creek) and a depth profile obtained from four sand samples yield 10Be exposure ages of 12.2 ± 1.5 and 11.3 ± 2.0 kyr, respectively. Both ages are in excellent agreement with the 14C age of 12.61 ± 0.20 cal kyr BP. In contrast, 10Be ages of five sandstone boulders vary significantly and exceed the age of the terrace by 10 to 70 kyr, which demonstrates that the widely used assumption of a negligible inherited component is not valid here. The age of the river terrace combined with the vertical fault offset yields an uplift rate of ~0.8 mm/yr for the Las Penas thrust. Combined with the fault dip of 25°, we determine a Late Quaternary horizontal shortening rate of ~1.8 mm/yr, which is about 40% of the GPS derived shortening rate of 4.5 ± 1.7 mm/yr in the back-arc region of the Andes (Brooks et al., 2003). References Brooks, B.A., Bevis, M., Smalley, R., Kendrick, E., Manceda, R., Lauria, E., Maturana, R. & Araujo, M. (2003): Crustal motion in the Southern Andes (26° - 36°S): Do the Andes behave like a microplate? Geochem. Geophys. Geosyst., 4 (10), pp. 14, 1085, doi 10.1029/2003GC000505. Costa, C.H., Gardini, C.E., Diederix, H., Cortés, J.M. (2000): The Andean orogenic

  10. A case of Distributed Continental Collision: Late Cretaceous Intraplate Shortening from Central Europe to North Africa

    Science.gov (United States)

    Kley, J.; Voigt, T.; Jaehne, F.

    2008-12-01

    Intraplate thrusting and basin inversion affected west-central Europe in Late Cretaceous time. The timing of this event is fairly well constrained between c. 90 and 65 Ma. The dominantly NW-trending European intraplate structures were often interpreted to have been dextrally transpressive, reflecting a northward push induced by the early collision of the Adria microplate with Europe's southern margin. However, many fault kinematic and other structural data from central Europe indicate dip-slip contraction essentially perpendicular to the main faults, suggesting a push from the southwest. In addition, recent plate reconstructions of the Mediterranean around 85 Ma place Adria far to the southeast and roughly along strike of the central European intraplate structures. The early Alpine nappe stack on Adria's leading edge was still separated from Europe by subducting oceanic lithosphere and had entered a phase of extension after the first orogenic event. All this makes Alpine collision an unlikely cause for intraplate thrusting in Europe. Rather, the timing, kinematics and location of structures suggest that intraplate shortening in Europe was a direct effect of convergence with the Iberian and African plates, with stresses transmitted across the Azores-Gibraltar fracture zone. This hypothesis is supported by structures of Late Cretaceous age indicating SW-NE to S-N shortening in France, Spain (particularly the onset of convergence in the Pyrenees) and northwestern Africa. In contrast to other examples such as the Laramides, intraplate thrusting in this case was not a foreland phenomenon related to a coeval orogen. It does not reflect a transition from subduction to continental collision, but the beginning of convergence across two former transform boundaries. This system which included no strongly thickened and weakened crust was mostly governed by far-field stresses and therefore responded rapidly to plate reorganizations. Specifically, the onset of thrusting

  11. Initial shortening and internal fixation in combination with a Sauvé-Kapandji procedure for severely comminuted fractures of the distal radius in elderly patients.

    Science.gov (United States)

    Arora, R; Gabl, M; Pechlaner, S; Lutz, M

    2010-11-01

    We identified 11 women with a mean age of 74 years (65 to 81) who sustained comminuted distal radial and ulnar fractures and were treated by volar plating and slight shortening of the radius combined with a primary Sauvé-Kapandji procedure. At a mean of 46 months (16 to 58), union of distal radial fractures and arthrodesis of the distal radioulnar joint was seen in all patients. The mean shortening of the radius was 12 mm (5 to 18) compared to the contralateral side. Flexion and extension of the wrist was a mean of 54° and 50°, respectively, and the mean pronation and supination of the forearm was 82° and 86°, respectively. The final mean disabilities of the arm, shoulder and hand score was 26 points. According to the Green and O'Brien rating system, eight patients had an excellent, two a good and one a fair result. The good clinical and radiological results, and the minor complications without the need for further operations related to late ulnar-sided wrist pain, justify this procedure in the elderly patient.

  12. THE EFFECT OF A STRETCH-SHORTENING CYCLE FATIGUE TEST ON THE DYNAMIC CHARACTERISTICS OF LOWER LIMBS IN ADULT MEN AND PRE-PUBESCENT BOYS

    Directory of Open Access Journals (Sweden)

    Ftikas C.

    2010-06-01

    Full Text Available Purpose: The present study focused on the acute effect differences between children and adults after a stretch shortening cycle fatigue test on drop jump performance.Method: Eleven pre-pubescent boys (10,2 ± 0,7 y old and eleven adult men (24,3 ± 3,3 y old performed a stretch shortening cycle fatigue test (SSFT,10 sets /10 repetitions, with 30 sec interval between sets. Before and after fatigue test, maximal isometric torque, drop jump (DJ, contact time and ground reaction forces (GRF wereevaluated. Fatigue perceives and feel of pain were evaluated immediately after fatigue as well.Results: After fatigue MVC and DJ significantly decreased in both groups but this decrease was higher in adults.Contact time and GRF were increased in both groups but in a higher extend in adults. Fatigue perception and the feeling of pain were also higher in adults.Conclusion: In this research, the SSFT resulted in acute reduction of the performance of both age groups butmore in adults. The higher performance reduction in adults could be attributed possibly both in neuromuscular, metabolic and inflammatory factors

  13. Shortened telomere length is demonstrated in T-cell subsets together with a pronounced increased telomerase activity in CD4 positive T cells from blood of patients with mycosis fungoides and parapsoriasis.

    Science.gov (United States)

    Wu, K D; Hansen, E R

    2001-10-01

    We have recently demonstrated that telomerase activity is increased and telomere length shortened in lymphocytes from peripheral blood of patients with cutaneous T-cell lymphoma. In order to determine which cell type has increased telomerase activity and shortened telomere length, CD4+, CD8+, CLA+ CD3+ and CLA- CD3+ T cells were isolated from peripheral blood of 25 patients, including 15 patients with mycosis fungoides and 10 patients with parapsoriasis. Eleven healthy individuals were used as controls; CD19+ B cells were separated from each individual as an internal control. The results showed that the increased telomerase activity was significantly predominating in the CD4+ T-cell subset. Significantly shortened telomere length was found in CD4+ and CD8+ T-cell subsets from the patients compared with the same cell subsets obtained from healthy individuals. However, no difference was observed between the subsets; CD19+ B cells collected from patients and healthy control individuals had similar telomerase activity and telomere length which was significantly different from the values found in T cells. The telomere length was significantly shorter in CLA+ CD3+ subset than in CLA- CD3+ subset. Interestingly, increased telomerase activity and shortened telomere length was also detected in CD4+ T cells from patients with parapsoriasis indicating that alteration of telomerase activity and telomere length in CD4+ T cells is an early event in the pathogenesis of cutaneous T-cell lymphoma. Thus, the results indicate that a significant high level of telomerase activity and shortened telomere length frequently occur in T cells of patients with CTCL and may reflect tumorigenesis.

  14. Changes in the soleus muscle architecture after exhausting stretch-shortening cycle exercise in humans.

    Science.gov (United States)

    Ishikawa, M; Dousset, E; Avela, J; Kyröläinen, H; Kallio, J; Linnamo, V; Kuitunen, S; Nicol, C; Komi, P V

    2006-06-01

    This study focused on the architectural changes in the muscle-tendon complex during the immediate and secondary (delayed) reductions of performance (bimodal recovery) caused by an exhaustive rebound type stretch-shortening cycle (SSC) exercise. The isometric plantar flexor torque during maximum voluntary contraction (MVC) was measured together with recording of electromyography (EMG) and ultrasonography from the soleus muscle before (BEF), after (AFT), 2 h (2H), 2 and 8 days (2D, 8D) after the SSC exercise (n=8). The performance variables (MVC torque and EMG activation) followed the bimodal recovery patterns. This was not the case in the changes of the fascicle length and muscle thickness. The relative torque changes in MVC correlated positively (R=0.78, P=0.02) to the corresponding averaged EMG changes between BEF and 2H (BEF-->2H); the significance disappeared in the comparison between 2H and 2D (2H-->2D), during which period MVC showed a secondary reduction. The relative torque changes in MVC showed no correlation with the changes in muscle thickness between BEF-2H. However, this correlation between 2H-2D was negative (R=-0.85, PMVC increased at 2H, and then decreased more at 2D than 2H (PMVC could be related to the increase in muscle volume.

  15. Inula Viscosa Extracts Induces Telomere Shortening and Apoptosis in Cancer Cells and Overcome Drug Resistance.

    Science.gov (United States)

    Merghoub, Nawal; El Btaouri, Hassan; Benbacer, Laila; Gmouh, Saïd; Trentesaux, Chantal; Brassart, Bertrand; Terryn, Christine; Attaleb, Mohammed; Madoulet, Claudie; Benjouad, Abdelaziz; Amzazi, Saaïd; El Mzibri, Mohammed; Morjani, Hamid

    2016-01-01

    Telomerase is activated in human papillomavirus (HPV) positive cervical cancer and targeting telomeres offers a novel anticancer therapeutic strategy. In this study, the telomere targeting properties, the cytotoxic as well as the pro-apoptotic effects of hexane (IV-HE) and dichloromethane (IV-DF) fractions from Inula viscosa L. extracts were investigated on human cervical HeLa and SiHa cancer cells. Our data demonstrate that IV-HE and IV-DF extracts were able to inhibit cell growth in HeLa and SiHa cells in a dose-dependent manner and studied resistant cell lines exhibited a resistance factor less than 2 when treated with the extracts. IV-HE and IV-DF extracts were able to inhibit telomerase activity and to induce telomere shortening as shown by telomeric repeat amplification protocol and TTAGGG telomere length assay, respectively. The sensitivity of fibroblasts to the extracts was increased when telomerase was expressed. Finally, IV-HE and IV-DF were able to induce apoptosis as evidenced by an increase in annexin-V labeling and caspase-3 activity. This study provides the first evidence that the IV-HE and IV-DF extracts from Inula viscosa L. target telomeres induce apoptosis and overcome drug resistance in tumor cells. Future studies will focus on the identification of the molecules involved in the anticancer activity.

  16. Remote ischaemic preconditioning shortens QT intervals during exercise in healthy subjects.

    Science.gov (United States)

    Caru, Maxime; Lalonde, François; Gravel, Hugo; Daigle, Chantal; Tournoux, François; Jacquemet, Vincent; Curnier, Daniel

    2016-11-01

    The protective action of remote ischaemic preconditioning (RIPC) has been demonstrated in the context of surgical interventions in cardiology. Application of RIPC to sports performance has been proposed, but its effect on the electrocardiogram (ECG) during exercise remains unknown. This exploratory study aims to measure the changes in ventricular repolarization observed during exercise following RIPC in healthy subjects. In an experimental randomized crossover study, 17 subjects underwent two bouts of constant load exercise tests at 75% and 115% of gas exchange threshold (GET). Prior to exercise, they were allocated to either control or RIPC intervention with four cycles of 5 min of ischaemia followed by 5 min of reperfusion. ECG was continuously recorded during the protocol. QT and RR intervals were measured every 30 s (on an average tracing of the preceding 10 s). Although the time course of RR intervals did not differ between the two interventions (p = .56 at 75% GET and p = .74 at 115% GET), a significant shortening of QT intervals (measured from Q onset to T end) was observed during exercise (mean ± standard deviation of RIPC vs. -32 ± 19 ms at 75% GET (p exercise.

  17. Masticatory efficiency of shortened dental arch subjects with removable partial denture: A comparative study.

    Science.gov (United States)

    Omo, J O; Sede, M A; Esan, T A

    2017-04-01

    The objective of this study was to compare the masticatory efficiency in subjects with shortened dental arch (SDA) before and after restoration with removable partial denture (RPD). This was a prospective study carried out on 36 consecutive patients. The subjects were asked to chew 5 g of a measured portion of fresh raw carrot for 20 specified numbers of strokes. The raw carrot was recovered into a cup and strained through a standard mesh sieve of 5 mm by 1 mm, it was air dried for 30 min and weighed with FEM digital series weighing scale. The masticatory performance ratio was then determined. The age range of the subjects was 34-64 years with the mean age being 52.2 ± 8.2 years. The difference between the total masticatory performance score at the post- and pre-treatment phases was statistically significant (P = 0.001). The improvement in masticatory performance was marked among the younger age groups (P = 0.001), unilateral free end saddle subjects (P = 0.001), and among the male gender (P < 0.05). Masticatory performance improved with the provision of RPD. However, the improvement was marked among the younger age groups, unilateral free end saddle subjects, and the male gender; thereby supporting the need for RPDs in patients with SDA.

  18. Genetic Heterogeneity of HER2 Amplification and Telomere Shortening in Papillary Thyroid Carcinoma

    Directory of Open Access Journals (Sweden)

    Paola Caria

    2016-10-01

    Full Text Available Extensive research is dedicated to understanding if sporadic and familial papillary thyroid carcinoma are distinct biological entities. We have previously demonstrated that familial papillary thyroid cancer (fPTC cells exhibit short relative telomere length (RTL in both blood and tissues and that these features may be associated with chromosome instability. Here, we investigated the frequency of HER2 (Human Epidermal Growth Factor Receptor 2 amplification, and other recently reported genetic alterations in sporadic PTC (sPTC and fPTC, and assessed correlations with RTL and BRAF mutational status. We analyzed HER2 gene amplification and the integrity of ALK, ETV6, RET, and BRAF genes by fluorescence in situ hybridization in isolated nuclei and paraffin-embedded formalin-fixed sections of 13 fPTC and 18 sPTC patients. We analyzed BRAFV600E mutation and RTL by qRT-PCR. Significant HER2 amplification (p = 0.0076, which was restricted to scattered groups of cells, was found in fPTC samples. HER2 amplification in fPTCs was invariably associated with BRAFV600E mutation. RTL was shorter in fPTCs than sPTCs (p < 0.001. No rearrangements of other tested genes were observed. These findings suggest that the association of HER2 amplification with BRAFV600E mutation and telomere shortening may represent a marker of tumor aggressiveness, and, in refractory thyroid cancer, may warrant exploration as a site for targeted therapy.

  19. Relationships between muscle power output using the stretch-shortening cycle and eccentric maximum strength.

    Science.gov (United States)

    Miyaguchi, Kazuyoshi; Demura, Shinichi

    2008-11-01

    This study aimed to examine the relationships between muscle power output using the stretch-shortening cycle (SSC) and eccentric maximum strength under elbow flexion. Eighteen young adult males pulled up a constant light load (2 kg) by ballistic elbow flexion under the following two preliminary conditions: 1) the static relaxed muscle state (SR condition), and 2) using the SSC with countermovement (SSC condition).Muscle power was determined from the product of the pulling velocity and the load mass by a power measurement instrument that adopted the weight-loading method. We assumed the pulling velocity to be the subject's muscle power parameters as a matter of convenience, because we used a constant load. The following two parameters were selected in reference to a previous study: 1) peak velocity (m x s(-1)) (peak power) and 2) 0.1-second velocity during concentric contraction (m x s(-1)) (initial power). Eccentric maximum strength by elbow flexion was measured by a handheld dynamometer.Initial power produced in the SSC condition was significantly larger than that in the SR condition. Eccentric maximum strength showed a significant and high correlation (r = 0.70) with peak power in the SSC condition but not in the SR condition. Eccentric maximum strength showed insignificant correlations with initial power in both conditions. In conclusion, it was suggested that eccentric maximum strength is associated with peak power in the SSC condition, but the contribution of the eccentric maximum strength to the SSC potentiation (initial power) may be low.

  20. Simulative Global Warming Negatively Affects Cotton Fiber Length through Shortening Fiber Rapid Elongation Duration.

    Science.gov (United States)

    Dai, Yanjiao; Yang, Jiashuo; Hu, Wei; Zahoor, Rizwan; Chen, Binglin; Zhao, Wenqing; Meng, Yali; Zhou, Zhiguo

    2017-08-23

    Global warming could possibly increase the air temperature by 1.8-4.0 °C in the coming decade. Cotton fiber is an essential raw material for the textile industry. Fiber length, which was found negatively related to the excessively high temperature, determines yarn quality to a great extent. To investigate the effects of global warming on cotton fiber length and its mechaism, cottons grown in artificially elevated temperature (34.6/30.5 °C, Tday/Tnight) and ambient temperature (31.6/27.3 °C) regions have been investigated. Becaused of the high sensitivities of enzymes V-ATPase, PEPC, and genes GhXTH1 and GhXTH2 during fiber elongation when responding to high temperature stress, the fiber rapid elongation duration (FRED) has been shortened, which led to a significant suppression on final fiber length. Through comprehensive analysis, Tnight had a great influence on fiber elongation, which means Tn could be deemed as an ideal index for forecasting the degree of high temperature stress would happen to cotton fiber property in future. Therefore, we speculate the global warming would bring unfavorable effects on cotton fiber length, which needs to take actions in advance for minimizing the loss in cotton production.

  1. NEW INSIGHTS INTO THE THEORY OF CROSS-BRIDGE TENSION OF STEADILY SHORTENING AND STRETCHING MUSCLES

    Directory of Open Access Journals (Sweden)

    KOKSHENEV V.B.

    2015-01-01

    Full Text Available Despite the great success, seminal Huxley's sliding filament model broadly fails to explain the steady state behavior of stretched activated skeletal muscles. Here, a new self-consistent solution to the fundamental kinetic equation for the distribution of actin-myosin linkages (cross-bridges is proposed in light of the generalized thermodynamic theory of fluctuations, thus substituting the transition state theory traditionally used for the transition rates. The unified description for mechanism of the force output in both shortening and stretching regimes is attributed to the interplay between the uniformly-state distributed, thermodynamically equilibrated myosin heads attached to actin filaments and asymmetrically-state distributed, mechanically equilibrated rotating myosin heads. The crossover between two steady regimes is associated with a reconstruction of the cross-bridge domains and change their attach-detach rates, leaving unchanged basic mechanical characteristics. Theory suggests a unified generic force-velocity equation, using only a single combination of the cross-bridge parameters for each regime, while Huxley's approach (1957 as well as its subsequent modifications, requires four adjustable parameters to fit the same data.

  2. Urban environment shortens telomere length in nestling great tits, Parus major.

    Science.gov (United States)

    Salmón, P; Nilsson, J F; Nord, A; Bensch, S; Isaksson, C

    2016-06-01

    Urban environments are expanding rapidly, and with urbanization come both challenges and opportunities for wildlife. Challenges include combating the anthropogenic disturbances such as light, noise and air pollution and lower availability of natural food sources. The benefits are many, including the availability of anthropogenic food sources, breeding boxes and warmer temperatures. Thus, depending on the context, urbanization can have both positive and negative effects on fitness related traits. It is well known that early-life conditions can have lifelong implications on fitness; little is however known about development in urban environments. We reciprocally cross-fostered urban and rural nestling great tits (Parus major L.) to study how growing up in an urban versus rural habitat affected telomere length (TL)-a suggested biomarker of longevity. We show, for the first time, that growing up in an urban environment significantly shortens TL, independently of natal origin (i.e. urban or rural). This implies that the urban environment imposes a challenge to developing birds, with potentially irreversible effects on lifespan.

  3. Active shortening within the Himalayan orogenic wedge implied by the 2015 Gorkha earthquake

    Science.gov (United States)

    Whipple, Kelin X.; Shirzaei, Manoochehr; Hodges, Kip V.; Ramon Arrowsmith, J.

    2016-09-01

    Models of Himalayan neotectonics generally attribute active mountain building to slip on the Himalayan Sole Thrust, also termed the Main Himalayan Thrust, which accommodates underthrusting of the Indian Plate beneath Tibet. However, the geometry of the Himalayan Sole Thrust and thus how slip along it causes uplift of the High Himalaya are unclear. We show that the geodetic record of the 2015 Gorkha earthquake sequence significantly clarifies the architecture of the Himalayan Sole Thrust and suggests the need for revision of the canonical view of how the Himalaya grow. Inversion of Gorkha surface deformation reveals that the Himalayan Sole Thrust extends as a planar gently dipping fault surface at least 20-30 km north of the topographic front of the High Himalaya. This geometry implies that building of the high range cannot be attributed solely to slip along the Himalayan Sole Thrust over a steep ramp; instead, shortening within the Himalayan wedge is required to support the topography and maintain rapid rock uplift. Indeed, the earthquake sequence may have included a moderate rupture (Mw 6.9) on an out-of-sequence thrust fault at the foot of the High Himalaya. Such internal deformation is an expected response to sustained, focused rapid erosion, and may be common to most compressional orogens.

  4. Deciphering oblique shortening of central Alborz in Iran using geodetic data

    Science.gov (United States)

    Vernant, Ph.; Nilforoushan, F.; Chéry, J.; Bayer, R.; Djamour, Y.; Masson, F.; Nankali, H.; Ritz, J.-F.; Sedighi, M.; Tavakoli, F.

    2004-06-01

    The Alborz is a narrow (100 km) and elevated (3000 m) mountain belt which accommodates the differential motion between the Sanandaj-Sirjan zone in central Iran and the South Caspian basin. GPS measurements of 12 geodetic sites in Central Alborz between 2000 and 2002 allow to constrain the motion of the belt with respect to western Eurasia. One site velocity on the Caspian shoreline suggests that the South Caspian basin moves northwest at a rate of 6±2 mm/year with respect to western Eurasia. North-South shortening across the Alborz occurs at 5±2 mm/year. To the South, deformation seems to extend beyond the piedmont area, probably due to active thrusting on the Pishva fault. We also observe a left-lateral shear of the overall belt at a rate of 4±2 mm/year, consistent with the geological motion observed along E-W active strike-slip faults inside the belt (e.g., the Mosha fault).

  5. The implementation of an Intensive Care Information System allows shortening the ICU length of stay.

    Science.gov (United States)

    Levesque, Eric; Hoti, Emir; Azoulay, Daniel; Ichai, Philippe; Samuel, Didier; Saliba, Faouzi

    2015-04-01

    Intensive care information systems (ICIS) implemented in intensive care unit (ICU) were shown to improve patient safety, reduce medical errors and increase the time devolved by medical/nursing staff to patients care. Data on the real impact of ICIS on patient outcome are scarce. This study aimed to evaluate the effects of ICIS on the outcome of critically-ill patients. From January 2004 to August 2006, 1,397 patients admitted to our ICU were enrolled in this observational study. This period was divided in two phases: before the implementation of ICIS (BEFORE) and after implementation of ICIS (AFTER). We compared standard ICU patient's outcomes: mortality, length of stay in ICU, hospital stay, and the re-admission rate depending upon BEFORE and AFTER. Although patients admitted AFTER were more severely ill than those of BEFORE (SAPS II: 32.1±17.5 vs. 30.5±18.5, p=0.014, respectively), their ICU length of stay was significantly shorter (8.4±15.2 vs. 6.8±12.9 days; p=0.048) while the re-admission rate and mortality rate were similar (4.4 vs. 4.2%; p=0.86, and 9.6 vs 11.2% p=0.35, respectively) in patients admitted AFTER. We observed that the implementation of ICIS allowed shortening of ICU length of stay without altering other patient outcomes.

  6. Respiratory acidosis prolongs, while alkalosis shortens, the duration and recovery time of vecuronium in humans.

    Science.gov (United States)

    Yamauchi, Masanori; Takahashi, Hiromi; Iwasaki, Hiroshi; Namiki, Akiyoshi

    2002-03-01

    To determine the effects of respiratory acidosis and alkalosis by mechanical ventilation on the onset, duration, and recovery times of vecuronium. Randomized, prospective study. Operating rooms in the Sapporo Medical University Hospital and Kitami Red Cross Hospital. 90 ASA physical status I and II patients undergoing lower abdominal surgery. Patients were randomly allocated to one of three groups by arterial carbon dioxide tension level (PaCO2; mmHg) after induction: hyperventilation group (PaCO2 = 25-35), normoventilation group (PaCO2 = 35-45), and hypoventilation group (PaCO2 = 45-55). Anesthesia was maintained by spinal block with inhalation of 50% to 66% nitrous oxide in oxygen and intermittent intravenous administration of fentanyl and midazolam with tracheal intubation. After vecuronium 0.08 mg/kg was given, onset, duration, and recovery time were measured by mechanomyography (Biometer Myograph 2,000, Odense, Denmark). There were significant differences in the duration and recovery time of vecuronium among the normoventilation group (12.7 +/- 3.3 min and 11.8 +/- 2.8 min, respectively), the hyperventilation group (10.6 +/- 3.5 min and 9.2 +/- 2.7 min, respectively; p respiratory acidosis and shortened in respiratory alkalosis.

  7. Accelerated Telomere Shortening and Replicative Senescence in Human Fibroblasts Overexpressing Mutant and Wild Type Lamin A

    Science.gov (United States)

    Huang, Shurong; Risques, Rosa Ana; Martin, George M.; Rabinovitch, Peter S.; Oshima, Junko

    2008-01-01

    LMNA mutations are responsible for a variety of genetic disorders, including muscular dystrophy, lipodystrophy, and certain progeroid syndromes, notably Hutchinson-Gilford Progeria. Although a number of clinical features of these disorders are suggestive of accelerated aging, it is not known whether cells derived from these patients exhibit cellular phenotypes associated with accelerated aging. We examined a series of isogenic skin fibroblast lines transfected with LMNA constructs bearing known pathogenic point mutations or deletion mutations found in progeroid syndromes. Fibroblasts overexpressing mutant lamin A exhibited accelerated rates of loss of telomeres and shortened replicative lifespans, in addition to abnormal nuclear morphology. To our surprise, these abnormalities were also observed in lines overexpressing wild-type lamin A. Copy number variants are common in human populations; those involving LMNA, whether arising meiotically or mitotically, might lead to progeroid phenotypes. In an initial pilot study of 23 progeroid cases without detectible WRN or LMNA mutations, however, no cases of altered LMNA copy number were detected. Nevertheless, our findings raise a hypothesis that changes in lamina organization may cause accelerated telomere attrition, with different kinetics for overexpession of wild-type and mutant lamin A, which leads to rapid replicative senescence and progroid phenotypes. PMID:17870066

  8. High-dose rifampicin kills persisters, shortens treatment duration, and reduces relapse rate in vitro and in vivo.

    Science.gov (United States)

    Hu, Yanmin; Liu, Alexander; Ortega-Muro, Fatima; Alameda-Martin, Laura; Mitchison, Denis; Coates, Anthony

    2015-01-01

    Although high-dose rifampicin holds promise for improving tuberculosis control by potentially shortening treatment duration, these effects attributed to eradication of persistent bacteria are unclear. The presence of persistent Mycobacterium tuberculosis was examined using resuscitation promoting factors (RPFs) in both in vitro hypoxia and in vivo murine tuberculosis models before and after treatment with incremental doses of rifampicin. Pharmacokinetic parameters and dose-dependent profile of rifampicin in the murine model were determined. The Cornell mouse model was used to test efficacy of high-dose rifampicin in combination with isoniazid and pyrazinamide and to measure relapse rate. There were large numbers of RPF-dependent persisters in vitro and in vivo. Stationary phase cultures were tolerant to rifampicin while higher concentrations of rifampicin eradicated plate count positive but not RPF-dependent persistent bacteria. In murine infection model, incremental doses of rifampicin exhibited a dose-dependent eradication of RPF-dependent persisters. Increasing the dose of rifampicin significantly reduced the risk of antibiotic resistance emergence. In Cornell model, mice treated with high-dose rifampicin regimen resulted in faster visceral clearance; organs were M. tuberculosis free 8 weeks post-treatment compared to 14 weeks with standard-dose rifampicin regimen. Organ sterility, plate count and RPF-dependent persister negative, was achieved. There was no disease relapse compared to the standard dose regimen (87.5%). High-dose rifampicin therapy results in eradication of RPF-dependent persisters, allowing shorter treatment duration without disease relapse. Optimizing rifampicin to its maximal efficacy with acceptable side-effect profiles will provide valuable information in human studies and can potentially improve current tuberculosis chemotherapy.

  9. High dose rifampicin kills persisters, shortens treatment duration and reduces relapse rate in vitro and in vivo

    Directory of Open Access Journals (Sweden)

    Yanmin eHu

    2015-06-01

    Full Text Available Although high-dose rifampicin holds promise for improving tuberculosis control by potentially shortening treatment duration, these effects attributed to eradication of persistent bacteria are unclear. The presence of persistent M. tuberculosis was examined using resuscitation promoting factors (RPF in both in vitro hypoxia and in vivo murine tuberculosis models before and after treatment with incremental doses of rifampicin. Pharmacokinetic parameters and dose-dependent profile of rifampicin in the murine model were determined. The Cornell mouse model was used to test efficacy of high-dose rifampicin in combination with isoniazid and pyrazinamide and to measure relapse rate. There were large numbers of RPF-dependent persisters in vitro and in vivo. Stationary phase cultures were tolerant to rifampicin while higher concentrations of rifampicin eradicated plate count positive but not RPF-dependent persistent bacteria. In murine infection model, incremental doses of rifampicin exhibited a dose-dependent eradication of RPF-dependent persisters. Increasing the dose of rifampicin significantly reduced the risk of antibiotic resistance emergence. In Cornell model, mice treated with high-dose rifampicin regimen resulted in faster visceral clearance; organs were M. tuberculosis free 8 weeks post-treatment compared to 14 weeks with standard-dose rifampicin regimen. Organ sterility, plate count and RPF-dependent persister negative, was achieved. There was no disease relapse compared to the standard dose regimen (87.5%. High-dose rifampicin therapy results in eradication of RPF-dependent persisters, allowing shorter treatment duration without disease relapse. Optimising rifampicin to its maximal efficacy with acceptable side-effect profiles will provide valuable information in human studies and can potentially improve current tuberculosis chemotherapy.

  10. Shortening Record in the Central Andean Plateau of Southern Peru: Basement Inversion, Thin-skinned Thrusting, and Geomorphic Response

    Science.gov (United States)

    Perez, N.; Horton, B. K.; McQuarrie, N.; Stübner, K.; Ehlers, T. A.

    2015-12-01

    Variations in the inherited structural architecture along western South America influence the timing, magnitude, and style of Andean deformation and topographic evolution. New results from mapping, balanced cross-section construction, apatite and zircon (U-Th)/He thermochronology, and geomorphology spanning the Altiplano, Eastern Cordillera and Subandean zone of southern Peru define the role of Triassic rift inversion on Cenozoic deformation style, thrust belt kinematics, exhumation timing, and spatial variation of canyon incision. A minimum of 130 km (38%) shortening along a 200 km transect accommodated by thin- and thick-skinned structures involved selective reactivation of Triassic normal faults that fed slip to shallower detachments of thin-skinned fold-thrust systems. Map relationships define unique structural domains defined by inherited normal faults. In the Eastern Cordillera, new zircon (U-Th)/He results from a Triassic pluton reveal Oligocene-Miocene (~26-18 Ma) cooling, consistent with previously reported exhumation from ~40 km along strike to the NW. However, rapid ~15 Ma cooling revealed by new apatite (U-Th)/He data is ~11 Myr older than equivalent rocks in a similar structural setting ~40 km to the NW. This suggests a potential middle Miocene shift from synchronous to diachronous exhumation along strike. Modern fluvial profiles constructed along the Eastern Cordillera show a reduction in knickpoint elevations from SE to NW, suggesting more youthful uplift toward the NW, comparable to the spatial trend in apatite (U-Th)/He ages. The deformation front reached the Subandean zone by ~15 Ma, after the majority of reported deformation in the Eastern Cordillera and Altiplano. These variations in exhumation over such distances are consistent with multiple potential scenarios: a northward propagating wave of uplift, spatial variations in climatically driven incision, interactions with mantle dynamics, or complex structural geometries.

  11. Choice reaction times for human head rotations are shortened by startling acoustic stimuli, irrespective of stimulus direction

    NARCIS (Netherlands)

    Oude Nijhuis, L.B.; Janssen, L.; Bloem, B.R.; Dijk, J.G. van; Gielen, C.C.A.M.; Borm, G.F.; Overeem, S.

    2007-01-01

    Auditory startle reflexes can accelerate simple voluntary reaction times (StartReact effect). To investigate the role of startle reflexes on more complex motor behaviour we formulated two questions: (1) can auditory startle reflexes shorten choice reaction times?; (2) is the StartReact effect

  12. Bit-Error-Rate-Minimizing Channel Shortening Using Post-FEQ Diversity Combining and a Genetic Algorithm

    Science.gov (United States)

    2009-03-10

    Mag., vol. 28, pp. 5–14, May 1990. 19. I. Medvedev and V. Tarokh, “A channel shortening multiuser detector for DS- CDMA systems,” in Proc. 53rd...OH 45433 Dr. Vasu Chakravarthy vasu.chakravarthy@wpafb.af.mil DSN :785-5579 x 4245 AFRL/RYRE Approval for public release; distribution is unlimited

  13. Effectiveness and cost-effectiveness of shortening rehabilitation programs for patients with chronic musculoskeletal pain; Design of a RCT

    NARCIS (Netherlands)

    Waterschoot, F.P.C.; Geertzen, J.H.B.; Dijkstra, P.U.; Reneman, M.F.

    2011-01-01

    Background and Aims: Pain Rehabilitation Programs (PRP's) are proven effective for patients with chronic musculoskeletal pain (CMP). Evidence about the relationship of dose on the effect of PRP however, is unavailable. We hypothesized that shortening PRP will not be inferior to care as usual. The ai

  14. Effects of shortening the dry period of dairy cows on milk production, energy balance, health, and fertility: A systemtic review

    NARCIS (Netherlands)

    Knegsel, van A.T.M.; Drift, van der S.G.A.; Cermáková, J.; Kemp, B.

    2013-01-01

    A dry period of 6–8 weeks for dairy cows is generally thought to maximise milk production in the next lactation. However, the value of such a long dry period is increasingly questioned. In particular, shortening the dry period shifts milk production from the critical period after calving to the

  15. Shortening delivery times of intensity modulated proton therapy by reducing proton energy layers during treatment plan optimization

    NARCIS (Netherlands)

    S. van de Water (Steven); H.M. Kooy; B.J.M. Heijmen (Ben); M.S. Hoogeman (Mischa)

    2015-01-01

    textabstractPurpose To shorten delivery times of intensity modulated proton therapy by reducing the number of energy layers in the treatment plan. Methods and Materials We have developed an energy layer reduction method, which was implemented into our in-house-developed multicriteria treatment

  16. Shortening delivery times of intensity modulated proton therapy by reducing proton energy layers during treatment plan optimization

    NARCIS (Netherlands)

    S. van de Water (Steven); H.M. Kooy; B.J.M. Heijmen (Ben); M.S. Hoogeman (Mischa)

    2015-01-01

    textabstractPurpose To shorten delivery times of intensity modulated proton therapy by reducing the number of energy layers in the treatment plan. Methods and Materials We have developed an energy layer reduction method, which was implemented into our in-house-developed multicriteria treatment plann

  17. Effects of shortening the dry period of dairy cows on milk production, energy balance, health, and fertility: A systemtic review

    NARCIS (Netherlands)

    Knegsel, van A.T.M.; Drift, van der S.G.A.; Cermáková, J.; Kemp, B.

    2013-01-01

    A dry period of 6–8 weeks for dairy cows is generally thought to maximise milk production in the next lactation. However, the value of such a long dry period is increasingly questioned. In particular, shortening the dry period shifts milk production from the critical period after calving to the week

  18. Enhanced Corticospinal Excitability and Volitional Drive in Response to Shortening and Lengthening Strength Training and Changes Following Detraining

    Science.gov (United States)

    Tallent, Jamie; Goodall, Stuart; Gibbon, Karl C.; Hortobágyi, Tibor; Howatson, Glyn

    2017-01-01

    There is a limited understanding of the neurological adaptations responsible for changes in strength following shortening and lengthening resistance training and subsequent detraining. The aim of the study was to investigate differences in corticospinal and spinal responses to resistance training of the tibialis anterior muscle between shortening or lengthening muscle contractions for 4 weeks and after 2 weeks of detraining. Thirty-one untrained individuals were assigned to either shortening or lengthening isokinetic resistance training (4 weeks, 3 days/weeks) or a non-training control group. Transcranial magnetic stimulation and peripheral nerve stimulation (PNS) were used to assess corticospinal and spinal changes, respectively, at pre-, mid-, post-resistance training and post detraining. Greater increases changes (P MVC were found from the respective muscle contraction training. Motor evoked potentials (expressed relative to background EMG) significantly increased in lengthening resistance training group under contraction intensities ranging from 25 to 80% of the shortening and lengthening contraction intensity (P MVC and V-wave did not change (P > 0.05), although MEP amplitude decreased during the detraining period (P < 0.01). No changes in H-reflex were found pre to post resistance training or post detraining. Modulation in V-wave appeared to be contraction specific, whereby greatest increases occurred following lengthening resistance training. Strength and volitional drive is maintained following 2 weeks detraining, however corticospinal excitability appears to decrease when the training stimulus is withdrawn. PMID:28223941

  19. Diagnostic accuracy of the original 30-item and shortened versions of the Geriatric Depression Scale in nursing home patients

    NARCIS (Netherlands)

    Jongenelis, K; Eisses, AMH; Gerritsen, DL; Beekman, ATF; Kluiter, H; Ribbe, MW

    2005-01-01

    Objective To determine the diagnostic accuracy of the 30-item and shortened versions of the Geriatric Depression Scale (GDS) in diagnosing depression in older nursing home patients. Method Three hundred and thirty-three older nursing home patients participated in a prospective cross-sectional study

  20. An observational cohort study on shortened dental arches--clinical course during a period of 27-35 years

    NARCIS (Netherlands)

    Gerritsen, A.E.; Witter, D.J.; Bronkhorst, E.M.; Creugers, N.H.J.

    2013-01-01

    OBJECTIVES: The objective of this study was to investigate the clinical course of shortened dental arches ('SDA group') compared to SDAs plus removable denture prosthesis ('SDA plus RDP group') and complete dental arches ('CDA group', controls). MATERIALS AND METHODS: Data (numbers of direct and

  1. Diagnostic accuracy of the original 30-item and shortened versions of the Geriatric Depression Scale in nursing home patients

    NARCIS (Netherlands)

    Jongenelis, K; Eisses, AMH; Gerritsen, DL; Beekman, ATF; Kluiter, H; Ribbe, MW

    2005-01-01

    Objective To determine the diagnostic accuracy of the 30-item and shortened versions of the Geriatric Depression Scale (GDS) in diagnosing depression in older nursing home patients. Method Three hundred and thirty-three older nursing home patients participated in a prospective cross-sectional study

  2. The stretch-shortening cycle : a model to study naturally occurring neuromuscular fatigue.

    Science.gov (United States)

    Nicol, Caroline; Avela, Janne; Komi, Paavo V

    2006-01-01

    Neuromuscular fatigue has traditionally been examined using isolated forms of either isometric, concentric or eccentric actions. However, none of these actions are naturally occurring in human (or animal) ground locomotion. The basic muscle function is defined as the stretch-shortening cycle (SSC), where the preactivated muscle is first stretched (eccentric action) and then followed by the shortening (concentric) action. As the SSC taxes the skeletal muscles very strongly mechanically, its influence on the reflex activation becomes apparent and very different from the isolated forms of muscle actions mentioned above. The ground contact phases of running, jumping and hopping etc. are examples of the SSC for leg extensor muscles; similar phases can also be found for the upper-body activities. Consequently, it is normal and expected that the fatigue phenomena should be explored during SSC activities. The fatigue responses of repeated SSC actions are very versatile and complex because the fatigue does not depend only on the metabolic loading, which is reportedly different among muscle actions. The complexity of SSC fatigue is well reflected by the recovery patterns of many neuromechanical parameters. The basic pattern of SSC fatigue response (e.g. when using the complete exhaustion model of hopping or jumping) is the bimodality showing an immediate reduction in performance during exercise, quick recovery within 1-2 hours, followed by a secondary reduction, which may often show the lowest values on the second day post-exercise when the symptoms of muscle soreness/damage are also greatest. The full recovery may take 4-8 days depending on the parameter and on the severity of exercise. Each subject may have their own time-dependent bimodality curve. Based on the reviewed literature, it is recommended that the fatigue protocol is 'completely' exhaustive to reduce the important influence of inter-subject variability in the fatigue responses. The bimodality concept is

  3. Desensitized morphological and cytokine response after stretch-shortening muscle contractions as a feature of aging in rats.

    Science.gov (United States)

    Rader, Erik P; Layner, Kayla N; Triscuit, Alyssa M; Kashon, Michael L; Gu, Ja K; Ensey, James; Baker, Brent A

    2015-12-01

    Recovery from contraction-induced injury is impaired with aging. At a young age, the secondary response several days following contraction-induced injury consists of edema, inflammatory cell infiltration, and segmental muscle fiber degeneration to aid in the clearance of damaged tissue and repair. This morphological response has not been wholly established at advanced age. Our aim was to characterize muscle fiber morphology 3 and 10 days following stretch-shortening contractions (SSCs) varying in repetition number (i.e. 0, 30, 80, and 150) for young and old rats. For muscles of young rats, muscle fiber degeneration was overt at 3 days exclusively after 80 or 150 SSCs and returned significantly closer to control values by 10 days. For muscles of old rats, no such responses were observed. Transcriptional microarray analysis at 3 days demonstrated that muscles of young rats differentially expressed up to 2144 genes while muscles of old rats differentially expressed 47 genes. Bioinformatic analysis indicated that cellular movement was a major biological process over-represented with genes that were significantly altered by SSCs especially for young rats. Protein levels in muscle for various cytokines and chemokines, key inflammatory factors for cell movement, increased 3- to 50-fold following high-repetition SSCs for young rats with no change for old rats. This age-related differential response was insightful given that for control (i.e. 0 SSCs) conditions, protein levels of circulatory cytokines/chemokines were increased with age. The results demonstrate ongoing systemic low-grade inflammatory signaling and subsequent desensitization of the cytokine/chemokine and morphological response to contraction-induced injury with aging - features which accompany age-related impairment in muscle recovery.

  4. Shortening Anesthesia Duration does not Affect Severity of Withdrawal Syndrome in Patients Undergoing Ultra Rapid Opioid Detoxification

    Directory of Open Access Journals (Sweden)

    Shoaleh Shami

    2010-02-01

    Full Text Available Ultra rapid opioid detoxification (UROD is one of the new methods of detoxification. This method of detoxification involves putting patients under general anesthesia and actively giving them opioid antagonists. The objective of this study was to evaluate effects of anesthesia duration in UROD on severity of withdrawal syndrome. Sixty addicted patients seeking UROD procedure assigned randomly to one of the 2hr, 4hr or 6hr anesthesia duration groups. Premedication and anesthesia procedure (induction and maintenance were the same for three groups. Detoxification was done for all patients with 50 mg oral naltroxane (prior to induction and 20 mg intravenous naloxane (8 mg/bolus and 12 mg/infusion. Blood pressure, heart rate and respiratory rate were automatically measured and recorded every 5 minutes. The severity of withdrawal syndrome was measured and recorded every one hour during anesthesia, 2hours post-anesthesia, and 12 and 24 hours following the induction of anesthesia according to the Wang Scale modified by Lomier (WSMBL. Patients aged 20-58 in three groups. Three cases experienced delirium after detoxification that lasted 24 hours in one. Severity of withdrawal syndrome in patients of groups 2, 4 and 6 hour were 8.7, 7.4 and 5.1 respectively during anesthesia and 12.3, 11.1 and 13.9 after 18 hours of anesthesia. Results of this study showed that, in standard settings, UROD is a safe method for detoxification and has low complications. The withdrawal symptoms during and after anesthesia are low. Shortening the duration of anesthesia has no affect on severity of withdrawal syndrome during and after anesthesia.

  5. Factors of force potentiation induced by stretch-shortening cycle in plantarflexors.

    Directory of Open Access Journals (Sweden)

    Atsuki Fukutani

    Full Text Available Muscle force is potentiated by countermovement; this phenomenon is called stretch-shortening cycle (SSC effect. In this study, we examined the factors strongly related to SSC effect in vivo, focusing on tendon elongation, preactivation, and residual force enhancement. Twelve healthy men participated in this study. Ankle joint angle was passively moved by a dynamometer, with a range of motion from 15° dorsiflexion (DF to 15° plantarflexion (PF. Muscle contraction was evoked by electrical stimulation, with stimulation timing adjusted to elicit three types of contraction: (1 concentric contraction without preliminary contraction (CON, (2 concentric contraction after preliminary eccentric contraction (ECC, and (3 concentric contraction after preliminary isometric contraction (ISO. Joint torque was recorded at DF5°, PF0°, and PF5°, respectively. SSC effect was calculated as the ratio of joint torque obtained in ECC or ISO with respect to that obtained in CON at the aforementioned three joint angles. SSC effect was prominent in the first half of movement in both ECC (DF5°, 329.3 ± 101.2%; PF0°, 159.2 ± 29.4%; PF5°, 125.5 ± 20.8% and ISO (DF5°, 276.4 ± 87.0%; PF0°, 134.5 ± 24.5%; PF5°, 106.8 ± 18.0% conditions. SSC effect was significantly larger in ECC than in ISO at all joint angles (P < 0.001. Even without preliminary eccentric contraction (i.e., ISO condition, SSC effect was clearly large, indicating that a significant part of SSC effect is derived from preactivation. However, the active lengthening-induced force potentiation mechanism (residual force enhancement also contributes to SSC effect.

  6. EMG signal amplitude normalization technique in stretch-shortening cycle movements.

    Science.gov (United States)

    Allison, G T; Marshall, R N; Singer, K P

    1993-01-01

    Analysis of functional movements using surface electromyography (EMG) often involves recording both eccentric and concentric muscle activity during a stretch-shorten cycle (SSC). The techniques used for amplitude normalization are varied and are independent of the type of muscle activity involved. The purpose of this study was: (i) to determine the effect of 11 amplitude normalization techniques on the coefficient of variation (CV) during the eccentric and concentric phases of the SSC; and (ii) to establish the effect of the normalization techniques on the EMG signal under variable load and velocity. The EMG signal of the biceps brachii of eight normal subjects was recorded under four SSC conditions and three levels of isometric contraction. The 11 derived normalization values were total rms, mean rms and peak rms (100 ms time constant) for the isometric contractions and the mean rms and peak rms values of the ensemble values for each set of isotonic contractions. Normalization using maximal voluntary isometric contractions (MVIC), irrespective of rms processing (total, mean or peak), demonstrated greater CV above the raw data for both muscle actions. Mean ensemble values and submaximal isometric recordings reduced the CV of concentric data. No amplitude normalization technique reduced the CV for eccentric data under loaded conditions. An ANOVA demonstrated significant (P concentric raw data and an interaction (P < 0.05) for raw eccentric data. No significant effects were demonstrated for changes in velocity when the data were normalized using mean rms values. The reduction of the CV should not be at the expense of true biological variance and current normalization techniques poorly serve the analysis of eccentric muscle activity during the SSC. Copyright © 1993. Published by Elsevier Ltd.

  7. Daidzin, an antioxidant isoflavonoid, decreases blood alcohol levels and shortens sleep time induced by ethanol intoxication.

    Science.gov (United States)

    Xie, C I; Lin, R C; Antony, V; Lumeng, L; Li, T K; Mai, K; Liu, C; Wang, Q D; Zhao, Z H; Wang, G F

    1994-12-01

    The extract from an edible vine, Pueraria lebata, has been reported to be efficacious in lessening alcohol intoxication. In this study, we have tested the efficacy of one of the major components, daidzin, from this plant extract. When ethanol (40% solution, 3 g/kg body weight) was given to fasted rats intragastrically, blood alcohol concentration (BAC) peaked at 30 min after alcohol ingestion and reached 1.77 +/- 0.14 mg/ml (mean values +/- SD, n = 6). If daidzin (30 mg/kg) was mixed with the ethanol solution and given to animals intragastrically, BAC was found to peak at 90 min after alcohol ingestion and reached only 1.20 +/- 0.30 mg/ml (n = 6) (p daidzin to delay and decrease peak BAC level after ethanol ingestion was also observed in fed animals. In both fasted and fed rats given alcohol without daidzin, BAC quickly declined after reaching its peak at 30 min. By contrast, BAC levels receded more slowly if daidzin was also fed to the animals. Daidzin showed a chronic effect. Rats fed daidzin for 7 days before ethanol challenge, but not on the day of challenge, also produced lower and later peak BAC levels. Interestingly, daidzin, whether fed to rats only once or chronically for 7 days, did not significantly alter activities of either alcohol dehydrogenase or mitochondrial aldehyde dehydrogenase in the liver. Further experiments demonstrated that daidzin shortened sleep time for rats receiving ethanol intragastrically (7 g/kg) but not intraperitoneally (2 g/kg). To test whether daidzin delayed stomach-emptying, [14C]polyethylene glycol was mixed with ethanol and fed to rats.(ABSTRACT TRUNCATED AT 250 WORDS)

  8. Possibility of stretch-shortening cycle movement training using a jump rope.

    Science.gov (United States)

    Miyaguchi, Kazuyoshi; Sugiura, Hiroki; Demura, Shinichi

    2014-03-01

    Although jumping rope has been said to be a typical stretch-shortening cycle movement (SSC) from the dynamic analysis of muscle contraction, there are few research reports that focus on this point. Recently, the function of SSC of the legs with respect to the jumping movement has been evaluated using the rebound jump index (RJ-index). This study aimed to examine the possibility of using rope jumping in SSC training by comparing the RJ-index of the rebound jump (standard value) and the 2 different methods of rope jumping. The subjects included 76 healthy young men. Most subjects were involved in routine sports training 2-3 times per week. They performed the rebound jump (5 consecutive vertical jumps) and both a basic and a double-under jump with the jump rope, according to each participant's individual style (rhythm or timing). The RJ-index was calculated using the ground contact time and the jump height. The reliabilities of the RJ-index in the basic (intraclass correlation coefficient: 0.85) and double-under jump (0.92) were high, and the RJ-index of the latter (1.34 ± 0.24) was significantly higher than that of the former (0.60 ± 0.21). In the case of a group with inferior SSC ability, the RJ-index of the rebound jump only showed a significant correlation with the double-under but not with the basic jump. When using the RJ-index (1.97 ± 0.38) of the rebound jump as a criterion, the double-under-using about 70% of the SSC ability-may be effective for reinforcement of SSC ability.

  9. Comparison of three shortened questionnaires for assessment of quality of life in advanced cancer.

    Science.gov (United States)

    Chiu, Leonard; Chiu, Nicholas; Chow, Edward; Cella, David; Beaumont, Jennifer L; Lam, Henry; Popovic, Marko; Bedard, Gillian; Poon, Michael; Wong, Erin; Zeng, Liang; Bottomley, Andrew

    2014-08-01

    Quality of life (QoL) assessment questionnaires can be burdensome to advanced cancer patients, thus necessitating the need for shorter assessment instruments than traditionally available. We compare three shortened QoL questionnaires in regards to their characteristics, validity, and reliability. A literature search was conducted to identify studies that employed or discussed three abridged QoL questionnaires: the European Organization for Research and Treatment of Cancer Quality of Life Core 15-Palliative Care (EORTC QLQ-C15-PAL), the Functional Assessment of Cancer Therapy-General-7 (FACT-G7), and the Functional Assessment of Chronic Illness Therapy-Palliative Care-14 (FACIT-PAL-14). Articles that discussed questionnaire length, intended use, scoring procedure, and validation were included. The 7-item FACT-G7 is the shortest instrument, whereas the EORTC QLQ-C15-PAL and the FACIT-PAL-14 contain 14 and 15 items, respectively. All three questionnaires have similar recall period, item organization, and subscale components. Designed as core questionnaires, all three maintain content and concurrent validity of their unabridged original questionnaires. Both the EORTC QLQ-C15-PAL and the FACT-G7 demonstrate good internal consistency and reliability, with Cronbach's α ≥0.7 deemed acceptable. The developmental study for the FACIT-PAL-14 was published in 2013 and subsequent validation studies are not yet available. The EORTC QLQ-C15-PAL and the FACT-G7 were found to be reliable and appropriate for assessing health-related QoL issues-the former for palliative cancer patients and the latter for advanced cancer patients receiving chemotherapy. Conceptually, the FACIT-PAL-14 holds promise to cover social and emotional support issues that are not completely addressed by the other two questionnaires; however, further validation is needed.

  10. Acute and prolonged reduction in joint stiffness in humans after exhausting stretch-shortening cycle exercise.

    Science.gov (United States)

    Kuitunen, S; Avela, J; Kyröläinen, H; Nicol, C; Komi, P V

    2002-11-01

    The purpose of the present study was to examine the acute and long-term fatigue effects of exhausting stretch-shortening cycle (SSC) exercise on the stiffness of ankle and knee joints. Five subjects were fatigued on a sledge apparatus by 100 maximal rebound jumps followed by continuous submaximal jumping until complete exhaustion. Neuromuscular fatigue effects were examined in submaximal hopping (HOP) and in maximal drop jumps (DJ) from 35 (DJ35) and 55 cm (DJ55) heights on a force plate. Additional force and reflex measurements were made using an ankle ergometer. Jumping tests and ankle ergometer tests were carried out before, immediately after, 2 h (2H), 2 days and 7 days (7D) after the SSC exercise. Kinematics, force and electromyography (EMG) recordings were complemented with inverse dynamics, which was used to calculate joint moments. The quotient of changes in joint moment divided by changes in joint angle was used as a value of joint stiffness (JS). In addition, blood lactate concentrations and serum creatine kinase activities were determined. The exercise induced a clear decrease in knee joint stiffness by [mean (SD)] 29 (13)% (P < 0.05) in HOP, 31 (6)% (P < 0.05) in DJ35 and 34 (14)% (P < 0.05) in DJ55. A similar trend was observed in the ankle joint stiffness with significant post-exercise reductions of 22 (8)% (P < 0.05) in DJ35 and of 27 (19)% (P < 0.05) at 2H in DJ55. The subsequent recovery of JS was slow and in some cases incomplete still at 7D. Generally, all the EMG parameters were fully recovered by 2H, whereas the force recovery was still incomplete at this time. These data indicate that the immediate reduction in JS was probably related to the effects of both central (neural) and peripheral (metabolic) fatigue, whereas the prolonged impairment was probably due to peripheral fatigue (muscle damage).

  11. Postsystolic Shortening Is Associated with Altered Right Ventricular Function in Children after Tetralogy of Fallot Surgical Repair

    Science.gov (United States)

    2017-01-01

    The aim of the study was to determine whether segmental interactions, as expressed by postsystolic shortening (PSS), affects RV mechanics and are connected with impaired systolic and diastolic function in rTOF children. Patients and Methods: 55 rTOF adolescent (study group), and 34 healthy volunteers (control group) were examined using classical Doppler flow (Doppler), Tissue Doppler Imaging (TDI) and Speckle Tracking Echocardiography (STE). PSS was found to occur when time to peak (TTP) was longer than pulmonary valve closure time (PVCT). TTP and strain were derived from RV lateral segments—basal (BL), medial (ML) and apical (AL) in STE. PVCT was measured from the beginning of QRS complex in the ECG to the termination of Doppler flow at the pulmonary valve. TDI was obtained at the lateral tricuspid annulus site and the systolic (S′), early (E′) and late diastolic (A’) peak velocities were measured along with isovolumic contraction (IVCT), and relaxation (IVRT) time. PW was used to measure early tricuspid inflow velocity (E) for calculating the E/E’ ratio. The TDI data in patients with PSS presence (TTP>PVCT) and those in whom it did not occur (TTP≤PVCT) were compared. Results: PSS in BL, ML and AL were observed respectively in: 27(51,9%), 9 (18%), and 8 (16,7%) patients. Mean values of TTP in BL, ML, and AL were respectively: 420.6±55.5ms, 389.8±50.0ms and 366.7±59.0ms. PVCT mean value was 396.6±33.5ms. In the study group, the mean E’ in TTP>PVCT was significantly lower (4.8±1.8 cm/s) compared to mean E’ in TTP≤PVCT (8.4±2.6 cm/s), pPCVT than in TTP≤PVCT, respectively 21.6±7.3 vs 12.2±5.1, pPVCT compared to IVRT in TTP≤PVCT, respectively 95.9±38.7 vs 77.0±35.1, pPVCT, significantly higher strain in BL (-28.8±8.7%) was observed when compared to that parameter in TTP≤PVCT (-35.3±13.1%), p <0.05. Conclusions: Tissue Doppler Echocardiography and Speckle Tracking Echocardiography are useful techniques for detecting regional systolic

  12. Shortening and intracellular Ca2+ in ventricular myocytes and expression of genes encoding cardiac muscle proteins in early onset type 2 diabetic Goto-Kakizaki rats.

    Science.gov (United States)

    Salem, K A; Adrian, T E; Qureshi, M A; Parekh, K; Oz, M; Howarth, F C

    2012-12-01

    .03 versus 0.80 ± 0.11; Kcna4, 0.79 ± 0.25 versus 1.90 ± 0.26; and Kcnj2, 0.52 ± 0.07 versus 0.78 ± 0.08) in GK ventricle compared with control ventricle. The amplitude of ventricular myocyte shortening and the intracellular Ca(2+) transient were unaltered; however, the time-to-peak shortening was prolonged and time-to-half decay of the Ca(2+) transient was shortened in GK myocytes compared with control myocytes. The results of this study demonstrate changes in expression of genes encoding various excitation-contraction coupling proteins that are associated with disturbances in myocyte shortening and intracellular Ca(2+) transport.

  13. Angioplasty of the pelvic and femoral arteries in PAOD: Results and review of the literature

    Energy Technology Data Exchange (ETDEWEB)

    Balzer, Joern O., E-mail: balzerjo@t-online.d [Dept. for Radiology and Nuclear medicine, Catholic Clinic Mainz, An der Goldgrube 11, 55131 Mainz (Germany); Thalhammer, Axel [Dept. of Diagnostic and Interventional Radiology, Univ. Clinic, Johann Wolfgang Goethe-University Frankfurt/Main (Germany); Khan, Verena [Dept. of Diagnostic and Interventional Radiology, Clinic Nuernberg North (Germany); Zangos, Stefan; Vogl, Thomas J.; Lehnert, Thomas [Dept. of Diagnostic and Interventional Radiology, Univ. Clinic, Johann Wolfgang Goethe-University Frankfurt/Main (Germany)

    2010-07-15

    Purpose: Evaluation of percutaneous recanalization of obstructed iliac as well as superficial femoral arteries (SFAs) in patients with peripheral arterial obstructive disease (PAOD). Material and methods: The data of 195 consecutive patients with 285 obstructions of the common and or external iliac artery as well as the data of 452 consecutive patients with 602 long occlusions (length > 5 cm) of the SFA were retrospectively analyzed. The lesions were either treated with percutaneous transluminal angioplasty (PTA) or Excimer laser assisted percutaneous transluminal angioplasty (LPTA). Overall 316 stents were implanted (Nitinol stents: 136; stainless steel stents: 180) in the iliac artery and 669 stents were implanted (Nitinol stents: 311; Easy Wallstents: 358) in the SFA. The follow-up period was 36-65 months (mean 46.98 {+-} 7.11 months) postinterventionally using clinical examination, ABI calculation, and color-coded duplex sonography. Patency rates were calculated on the basis of the Kaplan-Meier analysis. Results: The overall primary technical success rate was 97.89% for the iliac arteries and 92.35% for the SFA. Minor complications (hematoma, distal emboli and vessel dissection) were documented in 11.79% for the iliac arteries and 7.97% for the SFA. The primary patency rate was 90.3% for the iliac and 52.8% for the SFA after 4 years. The secondary patency rate was 96.84% for the iliac and 77.8% for the SFA after 4 years. Conclusion: Percutaneous recanalization of iliac and superficial femoral artery obstructions is a safe and effective technique for the treatment of patients with PAOD. By consequent clinical monitoring high secondary patency rates can be achieved. The use of a stents seems to result in higher patency rate especially in the SFA when compared to the literature in long-term follow-up.

  14. Septation and shortening of outflow tract in embryonic mouse heart involve changes in cardiomyocyte phenotype and α-SMA positive cells in the endocardium

    Institute of Scientific and Technical Information of China (English)

    杨艳萍; 李海荣; 景雅

    2004-01-01

    Background Studies on human, rat and chicken embryos have demonstrated that during the period of outflow tract septation, retraction of the distal myocardial margin of the outflow tract from the junction with aortic sac to the level of semilunar valves leads to the shortening of the myocardial tract. However, the mechanism is not clear. So we investigated the mechanism of outflow tract shortening and remodeling and the spatio-temporal distribution pattern of α-SMA positive cells in the outflow tract cushion during septation of the outflow tract in the embryonic mouse heart. Methods Serial sections of mouse embryos from embryonic day 9 (ED 9) to embryonic day 16 (ED 16) were stained with monoclonal antibodies against α-SCA, α-SMA, or desmin, while apoptosis was assessed using the terminal deoxyribonucleotidy transferase-mediated dUTP-digoxigenin nick-end labeling (TUNEL) assay. Results Between ED 11 and ED 12, the cardiomyocytes in the distal portion of the outflow tract were observed losing their myocardial phenotype without going into apoptosis, suggesting that trans-differentiation of cardiomyocytes into the cell components of the free walls of the intrapericardial ascending aorta and pulmonary trunk. The accumulation of α-SMA positive cells in the cardiac jelly began on ED 10 and participated in the ridge fusion and septation of the outflow tract. Fusion of the distal ridges resulted in the formation of the facing walls of the intrapericardial ascending aorta and pulmonary trunk. Fusion of the proximal ridges was accompanied by the accumulation of α-SMA positive cells into a characteristic central whorl, in which cell apoptosis could be observed. Subsequent myocardialization resulted in the formation of the partition between the subaortic and subpulmonary vestibules. Conclusions The shortening of the embryonic heart outflow tract in mice may result not from apoptosis, but from the trans-differentiation of cells with cardiomyocyte phenotype in the distal

  15. Decoupling of modern shortening rates, climate, and topography in the Caucasus

    Science.gov (United States)

    Forte, Adam M.; Whipple, Kelin X.; Bookhagen, Bodo; Rossi, Matthew W.

    2016-09-01

    The Greater and Lesser Caucasus mountains and their associated foreland basins contain similar rock types, experience a similar two-fold, along-strike variation in mean annual precipitation, and were affected by extreme base-level drops of the neighboring Caspian Sea. However, the two Caucasus ranges are characterized by decidedly different tectonic regimes and rates of deformation that are subject to moderate (less than an order of magnitude) gradients in climate, and thus allow for a unique opportunity to isolate the effects of climate and tectonics in the evolution of topography within active orogens. There is an apparent disconnect between modern climate, shortening rates, and topography of both the Greater Caucasus and Lesser Caucasus which exhibit remarkably similar topography along-strike despite the gradients in forcing. By combining multiple datasets, we examine plausible causes for this disconnect by presenting a detailed analysis of the topography of both ranges utilizing established relationships between catchment-mean erosion rates and topography (local relief, hillslope gradients, and channel steepness) and combining it with a synthesis of previously published low-temperature thermochronologic data. Modern climate of the Caucasus region is assessed through an analysis of remotely-sensed data (TRMM and MODIS) and historical streamflow data. Because along-strike variation in either erosional efficiency or thickness of accreted material fail to explain our observations, we suggest that the topography of both the western Lesser and Greater Caucasus are partially supported by different geodynamic forces. In the western Lesser Caucasus, high relief portions of the landscape likely reflect uplift related to ongoing mantle lithosphere delamination beneath the neighboring East Anatolian Plateau. In the Greater Caucasus, maintenance of high topography in the western portion of the range despite extremely low (<2-4 mm/y) modern convergence rates may be related

  16. Investigation of Thermal Conductivity and Heat Characteristics of Oil Sands Using Ultrasound Irradiation for Shortening the Preheating Time

    Science.gov (United States)

    Kamagata, Shingo; Kawamura, Youhei; Okawa, Hirokazu; Mizutani, Koichi

    2012-07-01

    Oil sands are attractive as an energy resource. Bitumen, which is found in oil sands, has high viscosity, so that it does not flow. Most oil sands are underground and are developed with a method called steam-assisted gravity drainage (SAGD). Hot steam is injected underground to fluidize bitumen and promote its recovery. However, the preheating time is too long. One way of reducing running costs is by shortening the preheating time. Previous studies have found that bitumen can be extracted from oil sands efficiently by applying ultrasonic irradiation, but SAGD was not applied directly in these cases. Thus, the purpose of this study is to apply ultrasonic irradiation to SAGD, thereby shortening the preheating time of oil sands. As a model experiment for SAGD, heat transfer experiments in a sand layer made with Toyoura sand and silicone oil were conducted and the thermal effect with ultrasound was investigated.

  17. A curve shortening flow rule for closed embedded plane curves with a prescribed rate of change in enclosed area.

    Science.gov (United States)

    Dallaston, Michael C; McCue, Scott W

    2016-01-01

    Motivated by a problem from fluid mechanics, we consider a generalization of the standard curve shortening flow problem for a closed embedded plane curve such that the area enclosed by the curve is forced to decrease at a prescribed rate. Using formal asymptotic and numerical techniques, we derive possible extinction shapes as the curve contracts to a point, dependent on the rate of decreasing area; we find there is a wider class of extinction shapes than for standard curve shortening, for which initially simple closed curves are always asymptotically circular. We also provide numerical evidence that self-intersection is possible for non-convex initial conditions, distinguishing between pinch-off and coalescence of the curve interior.

  18. Effect of shortened sleep on energy expenditure, core body temperature, and appetite: a human randomised crossover trial

    OpenAIRE

    Masanobu Hibi; Chie Kubota; Tomohito Mizuno; Sayaka Aritake; Yuki Mitsui; Mitsuhiro Katashima; Sunao Uchida

    2017-01-01

    The effects of sleep restriction on energy metabolism and appetite remain controversial. We examined the effects of shortened sleep duration on energy metabolism, core body temperature (CBT), and appetite profiles. Nine healthy men were evaluated in a randomised crossover study under two conditions: a 3.5-h sleep duration and a 7-h sleep duration for three consecutive nights followed by one 7-h recovery sleep night. The subjects’ energy expenditure (EE), substrate utilisation, and CBT were co...

  19. Crystallization and preliminary X-ray crystallographic analysis of yeast prion protein Ure2p with shortened N-terminal

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    An orthorhombic crystal form of a recombinant yeast prion protein with shortened N-terminal, 90Ure2p, has been obtained. Crystals were grown by the vapordiffusion technique against a mother liquor containing imidazole. Crystals belong to the primitive orthorhombic lattice with the cell parameters a = 54.5 ?, b = 74.7 ?, c = 131.0 ?. The crystals diffract to beyond 3.0 ? resolution at a synchrotron beamline.

  20. A continuum of myofibers in adult rabbit extraocular muscle: force, shortening velocity, and patterns of myosin heavy chain colocalization.

    Science.gov (United States)

    McLoon, Linda K; Park, Han Na; Kim, Jong-Hee; Pedrosa-Domellöf, Fatima; Thompson, Ladora V

    2011-10-01

    Extraocular muscle (EOM) myofibers do not fit the traditional fiber typing classifications normally used in noncranial skeletal muscle, in part, due to the complexity of their individual myofibers. With single skinned myofibers isolated from rectus muscles of normal adult rabbits, force and shortening velocity were determined for 220 fibers. Each fiber was examined for myosin heavy chain (MyHC) isoform composition by densitometric analysis of electrophoresis gels. Rectus muscle serial sections were examined for coexpression of eight MyHC isoforms. A continuum was seen in single myofiber shortening velocities as well as force generation, both in absolute force (g) and specific tension (kN/m(2)). Shortening velocity correlated with MyHCIIB, IIA, and I content, the more abundant MyHC isoforms expressed within individual myofibers. Importantly, single fibers with similar or identical shortening velocities expressed significantly different ratios of MyHC isoforms. The vast majority of myofibers in both the orbital and global layers expressed more than one MyHC isoform, with up to six isoforms in single fiber segments. MyHC expression varied significantly and unpredictably along the length of single myofibers. Thus EOM myofibers represent a continuum in their histological and physiological characteristics. This continuum would facilitate fine motor control of eye position, speed, and direction of movement in all positions of gaze and with all types of eye movements-from slow vergence movements to fast saccades. To fully understand how the brain controls eye position and movements, it is critical that this significant EOM myofiber heterogeneity be integrated into hypotheses of oculomotor control.

  1. To compare the efficacy of Drotaverine hydrochloride and Valethamate bromide in shortening of the first stage of labour

    Directory of Open Access Journals (Sweden)

    Sangeeta Raman Jogi

    2015-08-01

    In Group-II average rate was 1.42 cm/hour, in Primigravida it was 1.30 cm/hour and in Multigravida1.57 cm/hour. Conclusions: Drotaverine significantly shorten the duration of first stage of labor and found to be safe with no adverse effect on the mother and fetus. [Int J Reprod Contracept Obstet Gynecol 2015; 4(4.000: 1038-1043

  2. Ultrasonic investigation of the effect of vegetable shortening and mixing time on the mechanical properties of bread dough.

    Science.gov (United States)

    Mehta, K L; Scanlon, M G; Sapirstein, H D; Page, J H

    2009-01-01

    Mixing is a critical stage in breadmaking since it controls gluten development and nucleation of gas bubbles in the dough. Bubbles affect the rheology of the dough and largely govern the quality of the final product. This study used ultrasound (at a frequency where it is sensitive to the presence of bubbles) to nondestructively examine dough properties as a function of mixing time in doughs prepared from strong red spring wheat flour with various amounts of shortening (0%, 2%, 4%, 8% flour weight basis). The doughs were mixed for various times at atmospheric pressure or under vacuum (to minimize bubble nucleation). Ultrasonic velocity and attenuation (nominally at 50 kHz) were measured in the dough, and dough density was measured independently from specific gravity determinations. Ultrasonic velocity decreased substantially as mixing time increased (and more bubbles were entrained) for all doughs mixed in air; for example, in doughs made without shortening, velocity decreased from 165 to 105 ms(-1), although superimposed on this overall decrease was a peak in velocity at optimum mixing time. Changes in attenuation coefficient due to the addition of shortening were evident in both air-mixed and vacuum-mixed doughs, suggesting that ultrasound was sensitive to changes in the properties of the dough matrix during dough development and to plasticization of the gluten polymers by the shortening. Due to its ability to probe the effect of mixing times and ingredients on dough properties, ultrasound has the potential to be deployed as an online quality control tool in the baking industry.

  3. Effect of shortening replacement with flaxseed oil on physical, sensory, fatty acid and storage characteristics of cookies.

    Science.gov (United States)

    Rangrej, V; Shah, V; Patel, J; Ganorkar, P M

    2015-06-01

    Omega-3 fatty acid imparted good evidence of health benefits. Flaxseed oil, being the richest vegetarian source of alpha linolenic acid (omega-3 fatty acid), was incorporated in cookies by replacing shortening at level of 5 %, 10 %, 20 %, 30 %, 40 % and 50 %. Effect of shortening replacement with flaxseed oil on physical, textural and sensory attributes were investigated. Spread ratio and breaking strength of cookies increased as flaxseed oil level increased. Sensory score was not significantly affected up to 30 % shortening replacement with flaxseed oil as compared with the control cookies. Above 30 % flaxseed oil, sensory score was adversely affected. Fatty acid profile confirmed the enhancement of omega-3 fatty acid from 0 (control) to 14.14 % (30 % flaxseed oil cookies). The poly-unsaturated to saturated fatty acid ratio (P/S) increased from 0.088 (control) to 0.57 while ω - 6 to ω -3 fatty acid ratio of flaxseed oil cookies decreased from 4.51 (control) to 0.65 in the optimized cookies. The data on storage characteristics of the control and 30 % flaxseed oil cookies showed that there was significant change in the moisture content, Peroxide value (PV) and overall acceptability (OAA) up to 28 days of storage at 45 °C packed in polyethylene bags. Flaxseed oil cookies were acceptable up to 21 days of storage and afterwards noticeable off flavour was perceived.

  4. Intravenous Contrast Material Administration at High-pitch Dual-source CT Coronary Angiography: Bolus-tracking Technique with Shortened Time of Respiratory Instruction Versus Test Bolus Technique

    Institute of Scientific and Technical Information of China (English)

    Kai Sun; Guo-rong Liu; Yue-chun Li; Rui-juan Han; Li-fang Cui; Li-jun Ma; Li-gang Li; Chang-yong Li

    2012-01-01

    Objective To investigate the feasibility of acquiring the similar homogeneous enhancement using bolus-tracking techniques with shortened respiratory time in prospectively electrocardiogram-gated high-pitch spiral acquisition mode (Flash mode) coronary computed tomography angiography (CCTA) compared with test bolus technique.Methods One hundred and eighty-four consecutive patients with mean heart rate ≤65 beats per minute undergoing CCTA were prospectively included in this study.The patients were randomly divided into two groups.Patients in the group A (n=92) instructed to shorten respiratory time received CCTA using bolus-tracking technique with high-pitch spiral acquisition mode (Flash mode),while those in the group B (n=92) underwent CCTA with test bolus technique.The attenuation in the ascending aorta,image noise,contrast-to-noise ratio and radiation doses of the two groups were assessed.Results There were no significant differences in the mean attenuation values in the ascending aorta (483.18±59.07 HU vs.498.7±83.51 HU,P=0.183),image noise (21.4±4.5 HU vs.20.9±4.3 HU,P=0.414),contrast-to-noise ratio (12.1±4.2 vs.13.8±5.1,P=0.31) between the groups A and B.There were no significant differences in the radiation dose of dynamic monitoring scans (0.056±0.026 mSv vs.0.062±0.018 mSv,P=0.068) and radiation dose of angiography (0.94±0.07 mSv vs.0.96±0.15 mSv,P=0.926) between the two groups,while 15 mL less contrast material volume was administered in the group A than the group B.Conclusion Bolus-tracking technique with shortened time of respiratory in Flash mode of dual-source CT yields the similar homogeneous enhancement with less contrast material in comparison to the test bolus technique.

  5. Shortening of treatment duration in patients with chronic hepatitis C genotype 2 and 3 - impact of ribavirin dose - a randomized multicentre trial

    Directory of Open Access Journals (Sweden)

    Bischof Martin

    2011-06-01

    Full Text Available Abstract Background Chronic hepatitis C (CHC Patients, infected with genotype (GT 2 or 3 are treated with Peg-IFN and ribavirin (RBV (800 mg/day for 24 weeks. Treatment duration can be shortened to 12-16 weeks if a higher dose of RBV (1.000/1.200 mg/day was used without considerable loss of responsiveness or increased risk of relapse. Previously we have shown that in patients with CHC, GT 2/3 RBV can be reduced to 400 mg/day if administered for 24 weeks without an increase in relapse rates. Therefore we investigated the efficacy of a reduced RBV dosage of 400 mg/day with shorter treatment duration (16 weeks. Methods Treatment naïve patients with CHC, GT 2/3 were randomized to receive 180 μg peginterferonα2a/week in combination with either 800 (group C or 400 mg/d (group D for 16 weeks. The primary endpoint was SVR. Results 12 months after the first patient was randomized a inferior outcome of group D as compared to group C was noted, therefore the study was terminated. At study termination 89 patients were enrolled (group C: 31, D: 51. The SVR rate was statistically different in the two study groups with 51.6% in group C and 28.4% in group D (p = 0.038. Patients with low viral load had higher SVR rates (C: 67%, D: 33% than those with high viral load (C: 33%, D: 21%. Conclusion Both treatment duration and the dose of RBV play a major role to optimize outcome of patients with GT3. If one intends to shorten the treatment weight based RBV dose should be used, if lower RBV doses are used patients should be treated for at least 24 weeks as. A treatment regimen with a reduced RBV dosage and shortened treatment duration is associated with low SVR rates due to high relapse rates. Trial registration NCT01258101

  6. Preoperative Management of Surgical Patients by “Shortened Fasting Time”: A Study on the Amount of Total Body Water by Multi-Frequency Impedance Method

    Directory of Open Access Journals (Sweden)

    Hideki Taniguchi, Toshio Sasaki, Hisae Fujita

    2012-01-01

    Full Text Available Aim: Preoperative fasting is an established procedure to be practiced for patients before surgery, but optimal preoperative fasting time still remains controversial. The aim of this study was to investigate the effect of “shortened preoperative fasting time” on the change in the amount of total body water (TBW in elective surgical patients. TBW was measured by multi-frequency impedance method.Methods: The patients, who were scheduled to undergo surgery for stomach cancer, were divided into two groups of 15 patients each. Before surgery, patients in the control group were managed with conventional preoperative fasting time, while patients in the “enhanced recovery after surgery (ERAS” group were managed with “shortened preoperative fasting time” and “reduced laxative medication.” TBW was measured on the day before surgery and the day of surgery before entering the operating room. Defecation times and anesthesia-related vomiting and aspiration were monitored.Results: TBW values on the day of surgery showed changes in both groups as compared with those on the day before surgery, but the rate of change was smaller in the ERAS group than in the control group (2.4±6.8% [12 patients] vs. −10.6±4.6% [14 patients], p<0.001. Defecation times were less in the ERAS group. Vomiting and aspiration were not observed in either group.Conclusion: The results suggest that preoperative management with “shorted preoperative fasting time” and “reduced administration of laxatives” is effective in the maintenance of TBW in elective surgical patients.

  7. The transition between shortening and extensional regimes in central Mexico recorded in the tourmaline veins of the Comanja Granite

    Science.gov (United States)

    Angeles-Moreno, Edgar; Nieto-Samaniego, Angel Francisco; Ruiz-González, Francisco Jesús; Levresse, Gilles; Alaniz-Alvarez, Susana Alicia; Olmos Moya, María de Jesús Paulina; Xu, Shunshan; Miranda-Avilés, Raúl

    2017-01-01

    In central Mexico, there is a major angular unconformity separating two lithologic groups. Below the unconformity, the rocks display shortening deformation structures produced by the Laramide orogeny, overprinting those shortening structures there are normal faults related to the Cenozoic Basin and Range tectonics. Above the unconformity, the rocks are affected only by the Basin and Range tectonics, displaying mainly normal faults and in minor amount, lateral-oblique faults. We analyzed the Comanja Granite in the Sierra de Guanajuato, it is a large pluton that contains tourmaline veins. The Granite lacks of the shortening structures that pervasively affected the Mesozoic host-rocks; for this reason, we infer that the granite was formed after the main shortening event. We determined that the emplacement of the Comanja Granite took place between 51.0 ± 0.3 Ma and 49.5 ± 0.8 Ma, and that the tourmaline veins of the granite were formed at ∼51.0 Ma. At the microscopic scale, the tourmaline veins contain three kinds of tourmaline, called T1, T2, and T3, according to the order of their formation. The T1 tourmalines are brown colored and appear affected by brittle-ductile (D1) and brittle (D2) deformations. The cataclasites formed during D2 overprinted the brittle-ductile structures of D1, indicating the transition from deeper to shallower levels. In contrast, T2 and T3 tourmalines were not involved in those deformations. At the outcrop scale, we identified two slickensides in the tourmaline veins. The older, related to D1, is strike-slip with a small thrust component and the younger, related to D2, is normal with oblique components. The T1 tourmalines (which are deformed) were formed before the lateral-thrust faulting, whereas the T2 and T3 tourmalines, which are not deformed, were deposited after the faulting occurred in the veins. Our interpretation is that T1 tourmalines were deposited in the later phases of the Comanja Granite emplacement, with the minimum

  8. Deposition, clearance, and shortening of Kevlar para-aramid fibrils in acute, subchronic, and chronic inhalation studies in rats.

    Science.gov (United States)

    Kelly, D P; Merriman, E A; Kennedy, G L; Lee, K P

    1993-10-01

    The deposition and clearance of lung-deposited Kevlar para-aramid fibrils (subfibers) have been investigated as part of a subchronic and chronic inhalation toxicity testing program. Fibrils recovered from lung tissue in para-aramid-exposed Sprague-Dawley rats were microscopically counted and measured after exposures to airborne fibrils which were about 12 microns median length (ML) and < 0.3 micron median diameter. In each of three studies lung-recovered fibrils were progressively shorter with increasing residence time in the lungs. Twenty-eight days after a single 6-hr exposure at 400 respirable fibrils per cubic centimeter (f/cm3) the ML of recovered fibrils decreased to about 5 microns. Twenty-four months after a 3-week exposure to 25 or 400 f/cm3, fibrils reached about 2 microns ML. After 2 years of continuous exposure at 2.5, 25, or 100 f/cm3 or 1 year exposure plus 1 year recovery at 400 f/cm3, fibril ML approached 4 microns. In the 2-year study, the lung-fiber accumulation rate/exposure concentration was similar for the three highest concentrations and was about 3 x greater than that seen at 2.5 f/cm3, indicating that concentrations of about 25 f/cm3 or more may overwhelm clearance mechanisms. Time required for fibrils to be reduced to < 5 microns in the lung was markedly less at lower exposure concentration and shorter exposure time. The primary shortening mechanism is proposed to be long fibril cutting by enzymatic attack at fibril defects. However, length-selective fibril deposition and clearance may contribute to shortening in the first few days after exposure. The enzymatic cutting hypothesis is supported by measured increases in numbers of short fibers following cessation of exposures, continued shortening of the fibril length distribution up to 2 years following exposure, and in vitro fibril shortening after 3 months in a proteolytic enzyme preparation. The conclusion is that para-aramid fibrils are less durable in the lungs of rats than expected from

  9. Overexpression of the Large-Conductance, Ca2+-Activated K+ (BK) Channel Shortens Action Potential Duration in HL-1 Cardiomyocytes.

    Science.gov (United States)

    Stimers, Joseph R; Song, Li; Rusch, Nancy J; Rhee, Sung W

    2015-01-01

    Long QT syndrome is characterized by a prolongation of the interval between the Q wave and the T wave on the electrocardiogram. This abnormality reflects a prolongation of the ventricular action potential caused by a number of genetic mutations or a variety of drugs. Since effective treatments are unavailable, we explored the possibility of using cardiac expression of the large-conductance, Ca2+-activated K+ (BK) channel to shorten action potential duration (APD). We hypothesized that expression of the pore-forming α subunit of human BK channels (hBKα) in HL-1 cells would shorten action potential duration in this mouse atrial cell line. Expression of hBKα had minimal effects on expression levels of other ion channels with the exception of a small but significant reduction in Kv11.1. Patch-clamped hBKα expressing HL-1 cells exhibited an outward voltage- and Ca2+-sensitive K+ current, which was inhibited by the BK channel blocker iberiotoxin (100 nM). This BK current phenotype was not detected in untransfected HL-1 cells or in HL-1 null cells sham-transfected with an empty vector. Importantly, APD in hBKα-expressing HL-1 cells averaged 14.3 ± 2.8 ms (n = 10), which represented a 53% reduction in APD compared to HL-1 null cells lacking BKα expression. APD in the latter cells averaged 31.0 ± 5.1 ms (n = 13). The shortened APD in hBKα-expressing cells was restored to normal duration by 100 nM iberiotoxin, suggesting that a repolarizing K+ current attributed to BK channels accounted for action potential shortening. These findings provide initial proof-of-concept that the introduction of hBKα channels into a cardiac cell line can shorten APD, and raise the possibility that gene-based interventions to increase hBKα channels in cardiac cells may hold promise as a therapeutic strategy for long QT syndrome.

  10. Major temporal variations in shortening rate absorbed along a large active fold of the southeastern Tianshan piedmont (China)

    Science.gov (United States)

    Saint-Carlier, Dimitri; Charreau, Julien; Lavé, Jérôme; Blard, Pierre-Henri; Dominguez, Stéphane; Avouac, Jean-Philippe; Wang, Shengli

    2016-01-01

    The investigation of deformation rates on a mountain piedmont can provide key information for improving our understanding of the overall dynamics of a mountain range. Here, we estimate the shortening rate absorbed by a Quaternary emergent detachment fold on the southeastern piedmont of the Tianshan (China). Our work is primarily based on new 10Be cosmogenic exposure dating of deformed alluvial surfaces. The method we have developed combines depth profiling with sampling of surface cobbles, thereby allowing exposure time, erosion rate and inheritance to be simultaneously constrained. The exposure ages of the uppermost uplifted alluvial surfaces are around 140 ± 17 ka, 130 ± 9 ka and 47 ± 9 ka, from west to east. A terrace lying below the 140 ka surface is dated at 65 ± 5 ka. The ages of the uplifted and folded alluvial surfaces were then combined with estimates of shortening obtained using two distinct methods: (1) the excess area method, where sedimentation rates, extracted from magnetostratigraphic studies, are used to determine the amount of sedimentation after the abandonment of the river; and (2) a folding model derived from sandbox experiments. The late Pleistocene shortening rates are shown to be between 0.4 ± 0.1 mm /yr and 0.8 ± 0.5 mm /yr on the western part of the fold and 2.1 ± 0.4 mm /yr along its central part. The central part of the frontal Yakeng anticline therefore accommodates up to 25% of the total shortening currently absorbed across the whole Eastern Tianshan range (8 mm/yr). However, this situation seems to have prevailed for only the last 150 ka, as the shortening rate absorbed by this nascent fold was previously ten times slower. While the initiation of folding of the Yakeng anticline can be traced back to 5.5 Ma ago, the basinward migration of the active deformation front onto the Yakeng fold is a relatively recent phenomenon and appears to be diachronous from west to east, probably in relation to the tectonic activity of the folds in

  11. Unfavourable results in pollicisation

    Directory of Open Access Journals (Sweden)

    Mukund R Thatte

    2013-01-01

    Full Text Available Pollicisation of the index finger is perhaps one of the most complex and most rewarding operations in hand and plastic surgery. It however has a steep learning curve and demands very high skill levels and experience. There are multiple pitfalls and each can result in an unfavourable result. In essence we need to: Shorten the Index, recreate the carpo metacarpal joint from the metacarpo phalangeal (MP joint, rotate the digit by about 120° for pulp to pulp pinch, palmarly abduct by 40-50° to get a new first web gap, Shorten and readjust the tension of the extensors, re attach the intrinsics to form a thenar eminence capable of positioning the new thumb in various functional positions and finally close the flaps forming a new skin envelope. The author has performed over 75 pollicisations personally and has personal experience of some of the issues raised there. The steps mentioned therefore are an algorithm for helping the uninitiated into these choppy waters.

  12. Cenozoic foreland basin evolution during Andean shortening in the Malargüe region of western Argentina (35°S)

    Science.gov (United States)

    Ramirez, S. G.; Horton, B. K.; Fuentes, F.

    2015-12-01

    Cenozoic clastic deposits in western Argentina provide key opportunities to evaluate the timing and duration of Andean deformation and uplift. We studied the Malargüe segment of the Andean foreland basin at 35°S to better understand latest Cretaceous to Pliocene deformation and eastward propagation of Andean retroarc shortening. Our multi-technique approach included logging of a well-exposed ~1500m Paleocene-Miocene stratigraphic succession, paleocurrent measurements, conglomerate clast counts, and detrital zircon U-Pb geochronological analyses of basin fill exposed in the Sosneado region along the Rio Atuel. The Pircala and Coihueco Formations define the lowermost ~180 m of the section and are represented by fine to medium sandstones, siltstones, claystones and marls interpreted as distal fluvial floodplain and localized lacustrine deposits. Pircala paleocurrents show a major reversal from west- to east-directed flow. These finer deposits of the lower succession are separated from the overlying coarser-grained ~800 m thick Agua de la Piedra Formation by a conspicuous unconformity that spans up to roughly 20 Myr. The Agua de la Piedra Formation is composed of upward-coarsening amalgamated beds of massive medium to coarse sandstones and lenticular conglomerates interpreted as a prograding proximal fluvial to alluvial fan system. Conglomerate clast counts show initial dominance by Mesozoic detritus from the pre-Andean Neuquen basin system, with a progressive upsection increase in Cenozoic volcanic detritus from the Andean magmatic arc. Collectively, the paleocurrents, clast compositions, sedimentary facies associations, and emerging U-Pb results suggest a long-term shift, commencing in the Paleocene, from eastern cratonic sources to magmatic-arc and thrust-belt sources during a systematic eastward propagation of deformation, with a pronounced phase of Miocene magmatism and shortening that incorporated the proximal foreland basin into the advancing thrust belt.

  13. A Comparison of the Effects of Continuous versus Discontinuous Movement Patterns on Parkinsonian Rigidity and Reflex Responses to Passive Stretch and Shortening

    Science.gov (United States)

    Powell, Douglas; Muthumani, Anburaj; Xia, RuiPing

    2016-01-01

    Objective Quantify the effect of a continuous compared to discontinuous movement trajectory on parkinsonian rigidity and reflex responses to passive stretch and shortening. Methods Eighteen participants with Parkinson’s disease (PD) performed passive wrist flexion and extension movements through a 90° range of motion at 50 °/sec using continuous (CONT) and discontinuous (DISC) movement trajectories. Participants were tested in both the OFF-MED and ON-MED states. Rigidity was quantified by rigidity work score and slopes of the moment-angle plots during both flexion and extension. Reflex response was quantified by mean EMG amplitudes of forearm musculature. Results No differences were observed between CONT and DISC for rigidity (p = 0.18) or moment-angle plot slopes (Flexion: p = 0.97; Extension: p = 0.89). However, medication was associated with reductions in rigidity (p = 0.02) and increases in moment-angle plot slopes (Flexion: p = 0.03; Extension: p = 0.02). The CONT compared to DISC trajectory was associated with greater EMG amplitudes in the shortened muscles (p = 0.04) and smaller EMG ratios (p < 0.05) during flexion, and greater EMG amplitudes in the lengthened muscles (p = 0.02) during extension. Dopaminergic medication reduced EMG amplitudes in stretched muscles during extension (p < 0.05). Conclusions The nature of the movement trajectory (continuous vs. discontinuous) used during clinical assessment does not alter the presentation of rigidity in PD. Rigidity is reduced with the administration of dopaminergic medication, independent of movement trajectory. Significance These data suggest that the presentation of rigidity used in the determination of diagnosis, treatment and prognosis in PD will not be affected by the continuous nature of the movement trajectory used during clinical assessment.

  14. Non-contrast-enhanced hepatic MR angiography: Do two-dimensional parallel imaging and short tau inversion recovery methods shorten acquisition time without image quality deterioration?

    Energy Technology Data Exchange (ETDEWEB)

    Shimada, Kotaro, E-mail: kotaro@kuhp.kyoto-u.ac.jp [Department of Diagnostic Imaging and Nuclear Medicine, Kyoto University, Graduate School of Medicine, 54 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507 (Japan); Isoda, Hiroyoshi, E-mail: sayuki@kuhp.kyoto-u.ac.jp [Department of Diagnostic Imaging and Nuclear Medicine, Kyoto University, Graduate School of Medicine, 54 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507 (Japan); Okada, Tomohisa, E-mail: tomokada@kuhp.kyoto-u.ac.jp [Department of Diagnostic Imaging and Nuclear Medicine, Kyoto University, Graduate School of Medicine, 54 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507 (Japan); Kamae, Toshikazu, E-mail: toshi13@kuhp.kyoto-u.ac.jp [Department of Diagnostic Imaging and Nuclear Medicine, Kyoto University, Graduate School of Medicine, 54 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507 (Japan); Arizono, Shigeki, E-mail: arizono@kuhp.kyoto-u.ac.jp [Department of Diagnostic Imaging and Nuclear Medicine, Kyoto University, Graduate School of Medicine, 54 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507 (Japan); Hirokawa, Yuusuke, E-mail: yuusuke@kuhp.kyoto-u.ac.jp [Department of Diagnostic Imaging and Nuclear Medicine, Kyoto University, Graduate School of Medicine, 54 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507 (Japan); Shibata, Toshiya, E-mail: ksj@kuhp.kyoto-u.ac.jp [Department of Diagnostic Imaging and Nuclear Medicine, Kyoto University, Graduate School of Medicine, 54 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507 (Japan); Togashi, Kaori, E-mail: ktogashi@kuhp.kyoto-u.ac.jp [Department of Diagnostic Imaging and Nuclear Medicine, Kyoto University, Graduate School of Medicine, 54 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507 (Japan)

    2011-01-15

    Objective: To study whether shortening the acquisition time for selective hepatic artery visualization is feasible without image quality deterioration by adopting two-dimensional (2D) parallel imaging (PI) and short tau inversion recovery (STIR) methods. Materials and methods: Twenty-four healthy volunteers were enrolled. 3D true steady-state free-precession imaging with a time spatial labeling inversion pulse was conducted using 1D or 2D-PI and fat suppression by chemical shift selective (CHESS) or STIR methods. Three groups of different scan conditions were assigned and compared: group A (1D-PI factor 2 and CHESS), group B (2D-PI factor 2 x 2 and CHESS), and group C (2D-PI factor 2 x 2 and STIR). The artery-to-liver contrast was quantified, and the quality of artery visualization and overall image quality were scored. Results: The mean scan time was 9.5 {+-} 1.0 min (mean {+-} standard deviation), 5.9 {+-} 0.8 min, and 5.8 {+-} 0.5 min in groups A, B, and C, respectively, and was significantly shorter in groups B and C than in group A (P < 0.01). The artery-to-liver contrast was significantly better in group C than in groups A and B (P < 0.01). The scores for artery visualization and overall image quality were worse in group B than in groups A and C. The differences were statistically significant (P < 0.05) regarding the arterial branches of segments 4 and 8. Between group A and group C, which had similar scores, there were no statistically significant differences. Conclusion: Shortening the acquisition time for selective hepatic artery visualization was feasible without deterioration of the image quality by the combination of 2D-PI and STIR methods. It will facilitate using non-contrast-enhanced MRA in clinical practice.

  15. The application of PDCA in shortening the procurement time of small and medium sized medical equipment%PDCA在缩短中小型设备采购时间中的应用

    Institute of Scientific and Technical Information of China (English)

    陈志鹏; 彭都喜; 李越; 林正茂; 陈树鹏; 谭秋帆; 伍俊妍

    2015-01-01

    Objective To explore the role of PDCA in shortening the procurement time of small and medium sized medical equipment by quality control circles (QCC).Methods According to PDCA,we conducted QCC in small and medium sized medical equipment procurement in our hospital,analyzed the causes,set the goals,developed countermeasures,put them into effect,reviewed and improved steps of implementation.We used brainstorming,Gantt chart,fishbone diagram,flow chart management tools to shorten small and medium sized medical equipment procurement time.Result Through PDCA,the average procurement time of small and medium sized medical equipment was shortened by 40.12%.Conclusion The correct usage of PDCA can effectively shorten small and medium sized medical equipment procurement time,and improve the service ability of control circle members.%目的 运用品管圈活动,探索PDCA缩短中小型设备采购时间的作用.方法 根据PDCA的方法,开展品管圈活动,对我院的中小型设备采购进行现状分析、拟定对策、对策实施、检讨与改进等步骤的实施,运用头脑风暴、甘特图、鱼骨图、流程图等管理工具缩短中小型设备采购时间.结果 通过该项PDCA活动,我院中小型设备平均采购时间缩短40.12%.结论 正确运用PDCA循环可有效缩短中小型设备采购时间,并且能提高品管圈圈员的工作能力.

  16. Is the time between onset of pain and restoration of patency of infarct-related artery shortened in patients with myocardial infarction? The effects of the Kielce Region System for Optimal Management of Acute Myocardial Infarction

    Directory of Open Access Journals (Sweden)

    Marcin Sadowski

    2014-09-01

    Full Text Available Introduction : The importance of delay in the restoration of infarct-related artery patency in patients with myocardial infarction was discussed, and actions were undertaken in the Kielce Region aimed at shortening this time within the System for Optimal Management of Acute Myocardial Infarction. Aim of the research: To evaluate the effectiveness of shortening time delays during transport of patients and diagnostics of myocardial infarction in the Kielce Region. Material and methods: Time delays were analysed in 5,934 patients with ST-segment elevation myocardial infarction (STEMI, hospitalised in cardiology wards with interventional cardiology on 24-hour duty, during the period 2008–2012. Time delays were analysed between the onset of myocardial infarction pain and undertaking treatment – T1 and T2 time – within which a patient with myocardial infarction, after admission to hospital, has intervention performed on infarct-related coronary artery. Results : During the period 2008–2012, the median T1 time was successfully shortened from 355 to 203 min, and the T2 time from 101 to 48 min. Conclusions: The effectiveness of the system was confirmed, and the necessity for further improvement of the system indicated.

  17. Telomere shortening impairs regeneration of the olfactory epithelium in response to injury but not under homeostatic conditions.

    Directory of Open Access Journals (Sweden)

    Masami Watabe-Rudolph

    Full Text Available Atrophy of the olfactory epithelium (OE associated with impaired olfaction and dry nose represents one of the most common phenotypes of human aging. Impairment in regeneration of a functional olfactory epithelium can also occur in response to injury due to infection or nasal surgery. These complications occur more frequently in aged patients. Although age is the most unifying risk factor for atrophic changes and functional decline of the olfactory epithelium, little is known about molecular mechanisms that could influence maintenance and repair of the olfactory epithelium. Here, we analyzed the influence of telomere shortening (a basic mechanism of cellular aging on homeostasis and regenerative reserve in response to chemical induced injury of the OE in late generation telomere knockout mice (G3 mTerc(-/- with short telomeres compared to wild type mice (mTerc(+/+ with long telomeres. The study revealed no significant influence of telomere shortening on homeostatic maintenance of the OE during mouse aging. In contrast, the regenerative response to chemical induced injury of the OE was significantly impaired in G3 mTerc(-/- mice compared to mTerc(+/+ mice. Seven days after chemical induced damage, G3 mTerc(-/- mice exhibited significantly enlarged areas of persisting atrophy compared to mTerc(+/+ mice (p = 0.031. Telomere dysfunction was associated with impairments in cell proliferation in the regenerating epithelium. Deletion of the cell cycle inhibitor, Cdkn1a (p21 rescued defects in OE regeneration in telomere dysfunctional mice. Together, these data indicate that telomere shortening impairs the regenerative capacity of the OE by impairing cell cycle progression in a p21-dependent manner. These findings could be relevant for the impairment in OE function in elderly people.

  18. Rapid bioassay-guided screening of toxic substances in vegetable oils that shorten the life of SHRSP rats

    Directory of Open Access Journals (Sweden)

    Lewandowski Paul

    2010-02-01

    Full Text Available Abstract It has been consistently reported that vegetable oils including canola oil have a life shortening effect in Stroke-Prone Spontaneously Hypertensive Rats (SHRSP and this toxic effect is not due to the fatty acid composition of the oil. Although it is possible that the phytosterol content or type of phytosterol present in vegetable oils may play some role in the life shortening effect observed in SHRSP rats this is still not completely resolved. Furthermore supercritical CO2 fractionation of canola oil with subsequent testing in SHRSP rats identified safe and toxic fractions however, the compounds responsible for life shortening effect were not characterised. The conventional approach to screen toxic substances in oils using rats takes more than six months and involves large number of animals. In this article we describe how rapid bioassay-guided screening could be used to identify toxic substances derived from vegetable oils and/or processed foods fortified with vegetable oils. The technique incorporates sequential fractionation of oils/processed foods and subsequent treatment of human cell lines that can be used in place of animal studies to determine cytotoxicity of the fractions with structural elucidation of compounds of interest determined via HPLC-MS and GC-MS. The rapid bioassay-guided screening proposed would require two weeks to test multiple fractions from oils, compared with six months if animal experiments were used to screen toxic effects. Fractionation of oil before bio-assay enhances the effectiveness of the detection of active compounds as fractionation increases the relative concentration of minor components.

  19. Radiation and chemotherapy bystander effects induce early genomic instability events: Telomere shortening and bridge formation coupled with mitochondrial dysfunction

    Energy Technology Data Exchange (ETDEWEB)

    Gorman, Sheeona; Tosetto, Miriam [Centre for Colorectal Disease, St. Vincent' s University Hospital, Elm Park, Dublin 4 (Ireland); Lyng, Fiona; Howe, Orla [Radiation and Environmental Science Centre, Dublin Institute of Technology and St. Luke' s Hospital, Dublin (Ireland); Sheahan, Kieran; O' Donoghue, Diarmuid; Hyland, John; Mulcahy, Hugh [Centre for Colorectal Disease, St. Vincent' s University Hospital, Elm Park, Dublin 4 (Ireland); O' Sullivan, Jacintha, E-mail: jacintha.osullivan@ucd.ie [Centre for Colorectal Disease, St. Vincent' s University Hospital, Elm Park, Dublin 4 (Ireland)

    2009-10-02

    The bridge breakage fusion cycle is a chromosomal instability mechanism responsible for genomic changes. Radiation bystander effects induce genomic instability; however, the mechanism driving this instability is unknown. We examined if radiation and chemotherapy bystander effects induce early genomic instability events such as telomere shortening and bridge formation using a human colon cancer explant model. We assessed telomere lengths, bridge formations, mitochondrial membrane potential and levels of reactive oxygen species in bystander cells exposed to medium from irradiated and chemotherapy-treated explant tissues. Bystander cells exposed to media from 2 Gy, 5 Gy, FOLFOX treated tumor and matching normal tissue showed a significant reduction in telomere lengths (all p values <0.018) and an increase in bridge formations (all p values <0.017) compared to bystander cells treated with media from unirradiated tissue (0 Gy) at 24 h. There was no significant difference between 2 Gy and 5 Gy treatments, or between effects elicited by tumor versus matched normal tissue. Bystander cells exposed to media from 2 Gy irradiated tumor tissue showed significant depolarisation of the mitochondrial membrane potential (p = 0.012) and an increase in reactive oxygen species levels. We also used bystander cells overexpressing a mitochondrial antioxidant manganese superoxide dismutase (MnSOD) to examine if this antioxidant could rescue the mitochondrial changes and subsequently influence nuclear instability events. In MnSOD cells, ROS levels were reduced (p = 0.02) and mitochondrial membrane potential increased (p = 0.04). These events were coupled with a decrease in percentage of cells with anaphase bridges and a decrease in the number of cells undergoing telomere length shortening (p values 0.01 and 0.028 respectively). We demonstrate that radiation and chemotherapy bystander responses induce early genomic instability coupled with defects in mitochondrial function. Restoring

  20. Platinum(II) phenanthroimidazole G-quadruplex ligand induces selective telomere shortening in A549 cancer cells.

    Science.gov (United States)

    Mancini, Johanna; Rousseau, Philippe; Castor, Katherine J; Sleiman, Hanadi F; Autexier, Chantal

    2016-02-01

    Telomere maintenance, achieved by the binding of protective shelterin capping proteins to telomeres and by either telomerase or a recombination-based alternative lengthening of telomere (ALT) mechanism, is critical for cell proliferation and survival. Extensive telomere shortening or loss of telomere integrity activates DNA damage checkpoints, leading to cell senescence or death. Although telomerase upregulation is an attractive target for anti-cancer therapy, the lag associated with telomere shortening and the potential activation of ALT pose a challenge. An alternative approach is to modify telomere interactions with binding proteins (telomere uncapping). G-quadruplex ligands stabilize structures generated from single-stranded G-rich 3'-telomere end (G-quadruplex) folding, which in principle, cannot be elongated by telomerase, thus leading to telomere shortening. Ligands can also mediate rapid anti-proliferative effects by telomere uncapping. We previously reported that the G-quadruplex ligand, phenylphenanthroimidazole ethylenediamine platinum(II) (PIP), inhibits telomerase activity in vitro[47]. In the current study, a long-term seeding assay showed that PIP significantly inhibited the seeding capacity of A549 lung cancer cells and to a lesser extent primary MRC5 fibroblast cells. Importantly, treatment with PIP caused a significant dose- and time-dependent decrease in average telomere length of A549 but not MRC5 cells. Moreover, cell cycle analysis revealed a significant increase in G1 arrest upon treatment of A549 cells, but not MRC5 cells. Both apoptosis and cellular senescence may contribute to the anti-proliferative effects of PIP. Our studies validate the development of novel and specific therapeutic ligands targeting telomeric G-quadruplex structures in cancer cells.

  1. X-ray interference studies of crossbridge action in muscle contraction: evidence from muscles during steady shortening.

    Science.gov (United States)

    Huxley, Hugh; Reconditi, Massimo; Stewart, Alex; Irving, Tom

    2006-11-03

    During normal muscle shortening, the myosin heads must undergo many cycles of interaction with the actin filaments sliding past them. It is important to determine what range of configurations is found under these circumstances, and, in terms of the tilting lever arm model, what range of orientations the lever arms undergo. We have studied this using the X-ray interference technique described in the previous article, focusing mainly on the changes in the first order meridional reflection (M3) as compared to isometric. The change in ratio of the heights of the interference peaks indicates how far the mean lever arm angle has moved towards the end of the working stroke; the total intensity change depends on the angle change, on the number of heads now attached at any one time, and on the dispersion of lever arm angles. The latter provides a measure of the distance over which myosin heads remain attached to actin as they go through their working strokes. Surprisingly, the mean position of the attached heads moves only about 1 nm inwards (towards the center of the A-band) at low velocity shortening (around 0.9 T0): their dispersion changes very little. This shows that they must be detaching very early in the working stroke. However, at loads around 0.5 T0, the mean lever arm angle is about half way towards the end of the working stroke, and the dispersion of lever arm angles (with a uniform dispersion) is such as to distribute the heads throughout the whole of the working stroke. At higher velocities of shortening (at 0.3 T0), the mean position shifts further towards the end of the stroke, and the dispersion increases further. The details of the measurements, together with other data on muscle indicate that the force-generating mechanism within the myosin heads must have some unexpected properties.

  2. Rapid bioassay-guided screening of toxic substances in vegetable oils that shorten the life of SHRSP rats.

    Science.gov (United States)

    Ratnayake, Sunil; Lewandowski, Paul

    2010-02-02

    It has been consistently reported that vegetable oils including canola oil have a life shortening effect in Stroke-Prone Spontaneously Hypertensive Rats (SHRSP) and this toxic effect is not due to the fatty acid composition of the oil. Although it is possible that the phytosterol content or type of phytosterol present in vegetable oils may play some role in the life shortening effect observed in SHRSP rats this is still not completely resolved. Furthermore supercritical CO2 fractionation of canola oil with subsequent testing in SHRSP rats identified safe and toxic fractions however, the compounds responsible for life shortening effect were not characterised. The conventional approach to screen toxic substances in oils using rats takes more than six months and involves large number of animals. In this article we describe how rapid bioassay-guided screening could be used to identify toxic substances derived from vegetable oils and/or processed foods fortified with vegetable oils. The technique incorporates sequential fractionation of oils/processed foods and subsequent treatment of human cell lines that can be used in place of animal studies to determine cytotoxicity of the fractions with structural elucidation of compounds of interest determined via HPLC-MS and GC-MS. The rapid bioassay-guided screening proposed would require two weeks to test multiple fractions from oils, compared with six months if animal experiments were used to screen toxic effects. Fractionation of oil before bio-assay enhances the effectiveness of the detection of active compounds as fractionation increases the relative concentration of minor components.

  3. Shortened version of the work ability index to identify workers at risk of long-term sickness absence.

    Science.gov (United States)

    Schouten, Lianne S; Bültmann, Ute; Heymans, Martijn W; Joling, Catelijne I; Twisk, Jos W R; Roelen, Corné A M

    2016-04-01

    The Work Ability Index (WAI) identifies non-sicklisted workers at risk of future long-term sickness absence (LTSA). The WAI is a complicated instrument and inconvenient for use in large-scale surveys. We investigated whether shortened versions of the WAI identify non-sicklisted workers at risk of LTSA. Prospective study including two samples of non-sicklisted workers participating in occupational health checks between 2010 and 2012. A heterogeneous development sample (N= 2899) was used to estimate logistic regression coefficients for the complete WAI, a shortened WAI version without the list of diseases, and single-item Work Ability Score (WAS). These three instruments were calibrated for predictions of different (≥2, ≥4 and ≥6 weeks) LTSA durations in a validation sample of non-sicklisted workers (N= 3049) employed at a steel mill, differentiating between manual (N= 1710) and non-manual (N= 1339) workers. The discriminative ability was investigated by receiver operating characteristic analysis. All three instruments under-predicted the LTSA risks in both manual and non-manual workers. The complete WAI discriminated between individuals at high and low risk of LTSA ≥2, ≥4 and ≥6 weeks in manual and non-manual workers. Risk predictions and discrimination by the shortened WAI without the list of diseases were as good as the complete WAI. The WAS showed poorer discrimination in manual and non-manual workers. The WAI without the list of diseases is a good alternative to the complete WAI to identify non-sicklisted workers at risk of future LTSA durations ≥2, ≥4 and ≥6 weeks. © The Author 2015. Published by Oxford University Press on behalf of the European Public Health Association. All rights reserved.

  4. Telomere length shortening in gastric mucosa is a field effect associated with increased risk of gastric cancer.

    Science.gov (United States)

    Tahara, Tomomitsu; Shibata, Tomoyuki; Kawamura, Tomohiko; Horiguchi, Noriyuki; Okubo, Masaaki; Nakano, Naoko; Ishizuka, Takamitsu; Nagasaka, Mitsuo; Nakagawa, Yoshihito; Ohmiya, Naoki

    2016-07-01

    Telomere shortening occurs in many organs and tissues and is accelerated by oxidative injury and rapid cell turnover. Short telomeres initiate chromosomal instability and may eventually contribute to tumorigenesis. To evaluate telomere length as potential biomarker for gastric cancer (GC) risk, we measured average telomere length using quantitative real-time PCR in GC tissues and in non-neoplastic mucosa from patients with GC and without GC. We obtained of 217 GC patients matched biopsies from the GC and adjacent tissues as well as gastric biopsies of 102 subjects without GC. Relative telomere length was measured in genomic DNA by real-time PCR. Relative telomere length decreased gradually in Helicobacter pylori (H. pylori) negative and positive gastric mucosa of GC free subjects compared with adjacent mucosa and cancer tissue from GC patients (4.03 ± 0.3 vs. 2.82 ± 0.19 vs. 0.82 ± 0.07 vs. 0.29 ± 0.09, P telomeres were found significantly more often than in that of GC free subjects (age, sex, and H. pylori adjusted odds ratio = 7.81, 95 % confidence interval = 4.71-12.9, P Telomere shortening in non-neoplastic mucosa was associated with chronic inflammation (P = 0.0018) and intestinal metaplasia (P telomere length and clinicopathological features of GC and overall survival. Telomere shortening in gastric mucosa reflects a field effect in an early stage of carcinogenesis and is associated with an increased risk of GC. Telomere length in GC is not associated with clinicopathological features or prognosis.

  5. Radiation and chemotherapy bystander effects induce early genomic instability events: telomere shortening and bridge formation coupled with mitochondrial dysfunction.

    LENUS (Irish Health Repository)

    Gorman, Sheeona

    2012-02-01

    The bridge breakage fusion cycle is a chromosomal instability mechanism responsible for genomic changes. Radiation bystander effects induce genomic instability; however, the mechanism driving this instability is unknown. We examined if radiation and chemotherapy bystander effects induce early genomic instability events such as telomere shortening and bridge formation using a human colon cancer explant model. We assessed telomere lengths, bridge formations, mitochondrial membrane potential and levels of reactive oxygen species in bystander cells exposed to medium from irradiated and chemotherapy-treated explant tissues. Bystander cells exposed to media from 2Gy, 5Gy, FOLFOX treated tumor and matching normal tissue showed a significant reduction in telomere lengths (all p values <0.018) and an increase in bridge formations (all p values <0.017) compared to bystander cells treated with media from unirradiated tissue (0Gy) at 24h. There was no significant difference between 2Gy and 5Gy treatments, or between effects elicited by tumor versus matched normal tissue. Bystander cells exposed to media from 2Gy irradiated tumor tissue showed significant depolarisation of the mitochondrial membrane potential (p=0.012) and an increase in reactive oxygen species levels. We also used bystander cells overexpressing a mitochondrial antioxidant manganese superoxide dismutase (MnSOD) to examine if this antioxidant could rescue the mitochondrial changes and subsequently influence nuclear instability events. In MnSOD cells, ROS levels were reduced (p=0.02) and mitochondrial membrane potential increased (p=0.04). These events were coupled with a decrease in percentage of cells with anaphase bridges and a decrease in the number of cells undergoing telomere length shortening (p values 0.01 and 0.028 respectively). We demonstrate that radiation and chemotherapy bystander responses induce early genomic instability coupled with defects in mitochondrial function. Restoring mitochondrial

  6. Changes in conformation of myosin heads during the development of isometric contraction and rapid shortening in single frog muscle fibres.

    Science.gov (United States)

    Piazzesi, G; Reconditi, M; Dobbie, I; Linari, M; Boesecke, P; Diat, O; Irving, M; Lombardi, V

    1999-01-15

    1. Two-dimensional X-ray diffraction patterns were recorded at the European Synchrotron Radiation Facility from central segments of intact single muscle fibres of Rana temporaria with 5 ms time resolution during the development of isometric contraction. Shortening at ca 0.8 times the maximum velocity was also imposed at the isometric tetanus plateau. 2. The first myosin-based layer line (ML1) and the second myosin-based meridional reflection (M2), which are both strong in resting muscle, were completely abolished at the plateau of the isometric tetanus. The third myosin-based meridional reflection (M3), arising from the axial repeat of the myosin heads along the filaments, remained intense but its spacing changed from 14.34 to 14.56 nm. The intensity change of the M3 reflection, IM3, could be explained as the sum of two components, I14.34 and I14.56, arising from myosin head conformations characteristic of rest and isometric contraction, respectively. 3. The amplitudes (A) of the X-ray reflections, which are proportional to the fraction of myosin heads in each conformation, changed with half-times that were similar to that of isometric force development, which was 33.5 +/- 2. 0 ms (mean +/- s.d., 224 tetani from three fibres, 4 C), measured from the end of the latent period. We conclude that the myosin head conformation changes synchronously with force development, at least within the 5 ms time resolution of these measurements. 4. The changes in the X-ray reflections during rapid shortening have two temporal components. The rapid decrease in intensity of the 14.56 nm reflection at the start of shortening is likely to be due to tilting of myosin heads attached to actin. The slower changes in the other reflections were consistent with a return to the resting conformation of the myosin heads that was about 60 % complete after shortening of 70 nm per half-sarcomere.

  7. QRS-ST-T triangulation with repolarization shortening as a precursor of sustained ventricular tachycardia during acute myocardial ischemia.

    Science.gov (United States)

    Batchvarov, Velislav N; Behr, Elijah R

    2015-04-01

    We present segments from a 24-hour 12-lead digital Holter recording in a 48-year-old man demonstrating transient ST elevations in the inferior leads that triggered sustained ventricular tachycardia/ventricular fibrillation (VT/VF) requiring cardioversion. The onset of VT was preceded by a gradual increase in the ST with marked QRS broadening that lacked distinction between the end of the QRS and the beginning of the ST (QRS-ST-T "triangulation"), and shortening of the QT interval not caused by an increased heart rate. This is a relatively rare documentation of the mechanisms immediately triggering sustained ventricular arrhythmias during acute myocardial ischemia obtained with 12-lead ECG.

  8. Primary shortening of the forearm and Sauvé-Kapandji for severely comminuted fractures of the distal forearm in elderly patients: a case report.

    Science.gov (United States)

    Goorens, Chul Ki; Geurts, Ghislain; Goubau, Jean F

    2013-01-01

    We report a case of an elderly female who sustained a severely comminuted distal radial and ulnar fracture, treated by shortening of the forearm, combined with a primary Sauvé-Kapandji procedure and volar plating of the distal radius.

  9. A novel temperature control method for shortening thermal cycling time to achieve rapid polymerase chain reaction (PCR) in a disposable polymer microfluidic device

    DEFF Research Database (Denmark)

    Bu, Minqiang; R. Perch-Nielsen, Ivan; Sørensen, Karen Skotte

    We present a new temperature control method capable of effectively shortening the thermal cycling time of polymerase chain reaction (PCR) in a disposable polymer microfluidic device with external heater and temperature sensor. The method employs optimized temperature overshooting and undershooting...

  10. Effects of estrogen fluctuation during the menstrual cycle on the response to stretch-shortening exercise in females.

    Science.gov (United States)

    Sipavičienė, Saulė; Daniusevičiutė, Laura; Klizienė, Irina; Kamandulis, Sigitas; Skurvydas, Albertas

    2013-01-01

    The aim of this study was to investigate whether variation in estrogen levels during the menstrual cycle influences susceptibility to exercise-induced muscle damage after stretch-shortening cycle exercise. Physically active women (n = 18; age = 20.2 ± 1.7 yr) participated in this research. The subjects performed one session of 100 maximal drop jumps on day 1 or 2 of the follicular phase and another identical session on day 1 or 2 of the ovulatory phase; the order of the sessions was randomized. Quadriceps femoris muscle peak torque evoked by electrical stimulation and maximal voluntary contraction, muscle pain, and CK activity were measured before and at various times up to 72 h after exercise. It was found that the high estrogen level during the ovulatory phase might be related to an earlier return to baseline muscle strength after strenuous stretch-shortening cycle exercise in that phase compared with the follicular phase. The estrogen effect appears to be highly specific to the damaged site because the differences in most EIMD markers (CK, soreness, and low-frequency fatigue) between the two menstrual cycle phases were small.

  11. Effects of Estrogen Fluctuation during the Menstrual Cycle on the Response to Stretch-Shortening Exercise in Females

    Directory of Open Access Journals (Sweden)

    Saulė Sipavičienė

    2013-01-01

    Full Text Available The aim of this study was to investigate whether variation in estrogen levels during the menstrual cycle influences susceptibility to exercise-induced muscle damage after stretch-shortening cycle exercise. Physically active women (n=18; age = 20.2 ± 1.7 yr participated in this research. The subjects performed one session of 100 maximal drop jumps on day 1 or 2 of the follicular phase and another identical session on day 1 or 2 of the ovulatory phase; the order of the sessions was randomized. Quadriceps femoris muscle peak torque evoked by electrical stimulation and maximal voluntary contraction, muscle pain, and CK activity were measured before and at various times up to 72 h after exercise. It was found that the high estrogen level during the ovulatory phase might be related to an earlier return to baseline muscle strength after strenuous stretch-shortening cycle exercise in that phase compared with the follicular phase. The estrogen effect appears to be highly specific to the damaged site because the differences in most EIMD markers (CK, soreness, and low-frequency fatigue between the two menstrual cycle phases were small.

  12. Detrimental effects of reloading recovery on force, shortening velocity, and power of soleus muscles from hindlimb-unloaded rats.

    Science.gov (United States)

    Widrick, J J; Maddalozzo, G F; Hu, H; Herron, J C; Iwaniec, U T; Turner, R T

    2008-11-01

    To better understand how atrophied muscles recover from prolonged nonweight-bearing, we studied soleus muscles (in vitro at optimal length) from female rats subjected to normal weight bearing (WB), 15 days of hindlimb unloading (HU), or 15 days HU followed by 9 days of weight bearing reloading (HU-R). HU reduced peak tetanic force (P(o)), increased maximal shortening velocity (V(max)), and lowered peak power/muscle volume. Nine days of reloading failed to improve P(o), while depressing V(max) and intrinsic power below WB levels. These functional changes appeared intracellular in origin as HU-induced reductions in soleus mass, fiber cross-sectional area, and physiological cross-sectional area were partially or completely restored by reloading. We calculated that HU-induced reductions in soleus fiber length were of sufficient magnitude to overextend sarcomeres onto the descending limb of their length-tension relationship upon the resumption of WB activity. In conclusion, the force, shortening velocity, and power deficits observed after 9 days of reloading are consistent with contraction-induced damage to the soleus. HU-induced reductions in fiber length indicate that sarcomere hyperextension upon the resumption of weight-bearing activity may be an important mechanism underlying this response.

  13. Folder: a MATLAB-based tool for modelling deformation in layered media subject to layer parallel shortening or extension

    Science.gov (United States)

    Adamuszek, Marta; Dabrowski, Marcin; Schmid, Daniel W.

    2016-04-01

    We present Folder, a numerical tool to simulate and analyse the structure development in mechanically layered media during the layer parallel shortening or extension. Folder includes a graphical user interface that allows for easy designing of complex geometrical models, defining material parameters (including linear and non-linear rheology), and specifying type and amount of deformation. It also includes a range of features that facilitate the visualization and examination of various relevant quantities e.g. velocities, stress, rate of deformation, pressure, and finite strain. Folder contains a separate application, which illustrates analytical solutions of growth rate spectra for layer parallel shortening and extension of a single viscous layer. In the study, we also demonstrate a Folder application, where the role of confinement on the growth rate spectrum and the fold shape evolution during the deformation of a single layer subject to the layer parallel shortening is presented. In the case of the linear viscous materials used for the layer and matrix, the close wall proximity leads to a decrease of the growth rate values. The decrease is more pronounced for the larger wavelengths than for the smaller wavelengths. The growth rate reduction is greater when the walls are set closer to the layer. The presence of the close confinement can also affect the wavelength selection process and significantly shift the position of the dominant wavelength. The influence of the wall proximity on the growth rate spectrum for the case of non-linear viscous materials used for the layer and/or matrix is very different as compared to the linear viscous case. We observe a multiple maxima in the growth rate spectrum. The number of the growth rate maxima, their value and the position strongly depend on the closeness of the confinement. The maximum growth rate value for a selected range of layer-wall distances is much larger than in the case when the confinement effect is not taken

  14. Effect of shortened Integrated Management of Childhood Illness training on classification and treatment of under-five children seeking care in Rwanda

    Directory of Open Access Journals (Sweden)

    Harerimana JM

    2014-05-01

    Full Text Available Jean-Modeste Harerimana,1 Laetitia Nyirazinyoye,1 Jean-Bosco Ahoranayezu,2 Ferdinand Bikorimana,3 Bethany L Hedt-Gauthier,1,4 Katherine A Muldoon,5 Edward J Mills,6,7 Joseph Ntaganira1 1University of Rwanda College of Medicine and Health Sciences School of Public Health, Kigali, Rwanda; 2Community Vision Initiative, Kigali, Rwanda; 3Maternal and Child Health, Child Unit, Rwandan Ministry of Health, Kigali, Rwanda; 4Harvard Medical School, Boston, MA, USA; 5University of British Columbia, Vancouver, BC, Canada; 6University of Ottawa, Ottawa, ON, Canada; 7Stanford University, Stanford, CA, USA Background: Integrated Management of Childhood Illness (IMCI is an effective 11-day standard training; however, due to budgetary expenses and human resource constraints, many health professionals cannot take 11 days off work. As a result, shortened training curriculums (6-day have been proposed. We used a cross-sectional study to evaluate the effect of this shortened training on appropriate IMCI classification and treatment of under-five childhood illness management in Rwanda. Methods: A cross-sectional study was conducted in 22 health centers in Rwanda, comparing data from 121 nurses, where 55 nurses completed the 11-day and 66 nurses completed the 6-day training. Among 768 children, we evaluated clinical outcomes from May 2011 to April 2012. Descriptive statistics were used to display the sociodemographic characteristics of health providers; including level of education, sex, age, and professional experiences. Bivariable and multivariable analyses were used to test for differences between nurses in the 6-day versus 11-day training on the appropriate classification and treatment of childhood illness. Results: Our findings show that at the bivariable level and after controlling for confounders in the multivariable analysis, the only significant differences detected between nurses in the long and short training was the classification of fever (adjusted odds

  15. Feasibility of shortening isolation of TB-suspects by first-sample PCR

    DEFF Research Database (Denmark)

    Fløe, Andreas; Wejse, Christian; Thomsen, Vibeke Østergaard

    excluded. Objective: To evaluate the utility of single-specimen polymerase chain-reaction (PCR) for Mycobacterium tuberculosis complex (MTBC) as a parameter to cease isolation when negative. Methods: We evaluated all patients in Denmark who had sputa investigated for MTBC at the National Reference...... of the specimens PCR-analyzed for MTBC. We selected the first specimen with a PCR-result, and the next 2 specimens (regardless of PCR) within the 14-day window. From these, we identified the number of patients having at least one smear-positive specimen and a negative first PCR result. Results: We included 53......,533 sputa from 20,927 patients. In 4,208 samples, MTB was detected by culture, representing 1,637 patients. 858 of these patients had minimum 3 sputa analyzed within 14 days. Of these, 473 patients had at least one specimen analyzed by PCR. Overall, 342 (72.3%) had positive microscopy, and 372 (78.6%) had...

  16. Parallel Short Forms of the Crandall Social Desirability Test for Children: Shortening Instruments for Research Purposes.

    Science.gov (United States)

    Carifio, James

    Researchers and program evaluators would often like to use a particular instrument, but do not because it is too long or would require too much testing time. Having a validated set of objective procedures for reducing the size of an instrument could improve many research and evaluation efforts. This paper reports the results of test reduction or…

  17. Brave Upside Down World – Does Looking Between the Legs Elongate or Shorten the Perceived Distance

    Directory of Open Access Journals (Sweden)

    Oliver Tošković

    2010-03-01

    Full Text Available The aim of this research was to give further evidence for the influence of vestibularand proprioceptive information on distance perception, and to verify how perceived distancechanges if we use matching versus verbal judgment task. That is, the aim was to investigate how perceived distance changes when we bend over and look between the legs. The experiment was performed on a daylight open field (full-cue situation, with 20 participants, high school students from the Petnica Science Center. Participants had the task to match distances of two stimuli, one of which was in front of them at eye level, whereas the other was behind them, and they could observe it by bending their body forward and looking between the legs .Stimuli were 7cm*5cm large, and rectangular in shape. Results have shown that perceived distance changes in such a way that distances observed between the legs are perceived as longer than distances observed from an upright position. This difference in perceived distances exists only for larger physical distances (3m and 5m, but not for smaller physical distances (1m. Results coincide with our previous findings which indicate that vestibular and proprioceptive information change elongates perceived distance. On the other hand, the results contradict some findings gained in experiments in which the verbal judgment task was used. These contrary results probably appear because the verbal judgment task leaves more space for higher cognitive processes to be involved.

  18. NCI study finds extreme obesity may shorten life expectancy up to 14 years

    Science.gov (United States)

    Extremely obese people have increased risks of dying from cancer and many other causes including heart disease, stroke, diabetes, and kidney and liver diseases, according to results of an analysis of data pooled from 20 large studies of people from three

  19. The effect of strength training, recreational soccer and running exercise on stretch-shortening cycle muscle performance during countermovement jumping

    DEFF Research Database (Denmark)

    Jakobsen, Markus Due; Sundstrup, Emil; Randers, Morten Bredsgaard

    2012-01-01

    production, indicating a more explosive-type SSC muscle performance. No effects were detected in CMJ performance after continuous running, high-intensity interval running and recreational soccer, despite an increased muscle fiber CSA and quadriceps muscle activity in SOC. Enhanced neuromuscular activity......The purpose of the present study was to evaluate the effect of contrasting training modalities on mechanical muscle performance and neuromuscular activity during maximal SSC (stretch-shortening cycle) countermovement jumps (CMJ). Bilateral countermovement jumping, surface electromyography (EMG......) and muscle fiber size (CSA) were studied in untrained individuals (n=49, 21-45yrs) pre and post 12weeks of progressive heavy-resistance strength training (ST, n=8), recreational soccer training (SOC, n=15), high-intensity interval running (INT, n=7), continuous running (RUN, n=9) or continuation...

  20. Characteristics of myocardial postsystolic shortening in patients with symptomatic hypertrophic obstructive cardiomyopathy before and half a year after alcohol septal ablation assessed by speckle tracking echocardiography.

    Directory of Open Access Journals (Sweden)

    Jiansong Yuan

    Full Text Available OBJECTIVES: Postsystolic shortening (PSS has been proposed as a marker of myocardial dysfunction. Percutaneous transluminal septal myocardial ablation (PTSMA is an alternative therapy for patients with hypertrophic obstructive cardiomyopathy (HOCM that results in sustained improvements in atrial structure and function. We investigated the effects of PTSMA on PSS in HOCM patients using speckle tracking imaging. METHODS: Conventional echocardiographic and PSS parameters were obtained in 18 healthy controls and 30 HOCM patients before and half a year after PTSMA. RESULTS: Compared with the healthy controls, the number of segments having PSS and the average value of PSS were significantly increased in the HOCM patients. At 6 months after PTSMA, both the number of segments having PSS (10.5±2.8 vs. 13.2±2.6; P<0.001 and the average value of PSS (-1.24±0.57 vs. -1.55±0.56; P = 0.009 were significantly reduced. Moreover, the reductions in the average value of PSS correlated well with the reductions in the E-to-Ea ratio (r = 0.705, P<0.001. CONCLUSIONS: Both the number of segments having PSS and the average value of PSS were significantly increased in the HOCM patients. PTSMA has a favourable effect on PSS, which may partly account for the persistent improvement in LV diastolic function in HOCM patients after PTSMA.

  1. Chromosomal instability and telomere shortening in long-term culture of hematopoietic stem cells: insights from a cell culture model of RPS14 haploinsufficiency.

    Science.gov (United States)

    Thomay, K; Schienke, A; Vajen, B; Modlich, U; Schambach, A; Hofmann, W; Schlegelberger, B; Göhring, G

    2014-01-01

    The fate of cultivated primary hematopoietic stem cells (HSCs) with respect to genetic instability and telomere attrition has not yet been described in great detail. Thus, knowledge of the genetic constitution of HSCs is important when interpreting results of HSCs in culture. While establishing a cell culture model for myelodysplastic syndrome with a deletion in 5q by performing RPS14 knockdown, we found surprising data that may be of importance for any CD34+ cell culture experiments. We performed cytogenetic analyses and telomere length measurement on transduced CD34+ cells and untransduced control cells to observe the effects of long-term culturing. Initially, CD34+ cells had a normal median telomere length of about 12 kb and showed no signs of chromosomal instability. During follow-up, the median telomere length seemed to decrease and, simultaneously, increased chromosomal instability could be observed - in modified and control cells. One culture showed a clonal monosomy 7 - independent of prior RPS14 knockdown. During further culturing, it seemed that the telomeres re-elongated, and chromosomes stabilized, while TERT expression was not elevated. In summary, irrespective of our results of RPS14 knockdown in the long-term culture of CD34+ cells, it becomes clear that cell culture artefacts inducing telomere shortening and chromosomal instability have to be taken into account and regular cytogenetic analyses should always be performed.

  2. Running rescues defective adult neurogenesis by shortening the length of the cell cycle of neural stem and progenitor cells.

    Science.gov (United States)

    Farioli-Vecchioli, Stefano; Mattera, Andrea; Micheli, Laura; Ceccarelli, Manuela; Leonardi, Luca; Saraulli, Daniele; Costanzi, Marco; Cestari, Vincenzo; Rouault, Jean-Pierre; Tirone, Felice

    2014-07-01

    Physical exercise increases the generation of new neurons in adult neurogenesis. However, only few studies have investigated the beneficial effects of physical exercise in paradigms of impaired neurogenesis. Here, we demonstrate that running fully reverses the deficient adult neurogenesis within the hippocampus and subventricular zone of the lateral ventricle, observed in mice lacking the antiproliferative gene Btg1. We also evaluated for the first time how running influences the cell cycle kinetics of stem and precursor subpopulations of wild-type and Btg1-null mice, using a new method to determine the cell cycle length. Our data show that in wild-type mice running leads to a cell cycle shortening only of NeuroD1-positive progenitor cells. In contrast, in Btg1-null mice, physical exercise fully reactivates the defective hippocampal neurogenesis, by shortening the S-phase length and the overall cell cycle duration of both neural stem (glial fibrillary acidic protein(+) and Sox2(+)) and progenitor (NeuroD1(+)) cells. These events are sufficient and necessary to reactivate the hyperproliferation observed in Btg1-null early-postnatal mice and to expand the pool of adult neural stem and progenitor cells. Such a sustained increase of cell proliferation in Btg1-null mice after running provides a long-lasting increment of proliferation, differentiation, and production of newborn neurons, which rescues the impaired pattern separation previously identified in Btg1-null mice. This study shows that running positively affects the cell cycle kinetics of specific subpopulations of newly generated neurons and suggests that the plasticity of neural stem cells without cell cycle inhibitory control is reactivated by running, with implications for the long-term modulation of neurogenesis.

  3. Higher education delays and shortens cognitive impairment: a multistate life table analysis of the US Health and Retirement Study.

    Science.gov (United States)

    Reuser, Mieke; Willekens, Frans J; Bonneux, Luc

    2011-05-01

    Improved health may extend or shorten the duration of cognitive impairment by postponing incidence or death. We assess the duration of cognitive impairment in the US Health and Retirement Study (1992-2004) by self reported BMI, smoking and levels of education in men and women and three ethnic groups. We define multistate life tables by the transition rates to cognitive impairment, recovery and death and estimate Cox proportional hazard ratios for the studied determinants. 95% confidence intervals are obtained by bootstrapping. 55 year old white men and women expect to live 25.4 and 30.0 years, of which 1.7 [95% confidence intervals 1.5; 1.9] years and 2.7 [2.4; 2.9] years with cognitive impairment. Both black men and women live 3.7 [2.9; 4.5] years longer with cognitive impairment than whites, Hispanic men and women 3.2 [1.9; 4.6] and 5.8 [4.2; 7.5] years. BMI makes no difference. Smoking decreases the duration of cognitive impairment with 0.8 [0.4; 1.3] years by high mortality. Highly educated men and women live longer, but 1.6 years [1.1; 2.2] and 1.9 years [1.6; 2.6] shorter with cognitive impairment than lowly educated men and women. The effect of education is more pronounced among ethnic minorities. Higher life expectancy goes together with a longer period of cognitive impairment, but not for higher levels of education: that extends life in good cognitive health but shortens the period of cognitive impairment. The increased duration of cognitive impairment in minority ethnic groups needs further study, also in Europe.

  4. The endothermic ATP hydrolysis and crossbridge attachment steps drive the increase of force with temperature in isometric and shortening muscle.

    Science.gov (United States)

    Offer, Gerald; Ranatunga, K W

    2015-04-15

    The isometric tetanic tension of skeletal muscle increases with temperature because attached crossbridge states bearing a relatively low force convert to those bearing a higher force. It was previously proposed that the tension-generating step(s) in the crossbridge cycle was highly endothermic and was therefore itself directly targeted by changes in temperature. However, this did not explain why a rapid rise in temperature (a temperature jump) caused a much slower rate of rise of tension than a rapid length step. This led to suggestions that the step targeted by a temperature rise is not the tension-generating step but is an extra step in the attached pathway of the crossbridge cycle, perhaps located on a parallel pathway. This enigma has been a major obstacle to a full understanding of the operation of the crossbridge cycle. We have now used a previously developed mechano-kinetic model of the crossbridge cycle in frog muscle to simulate the temperature dependence of isometric tension and shortening velocity. We allowed all five steps in the cycle to be temperature-sensitive. Models with different starting combinations of enthalpy changes and activation enthalpies for the five steps were refined by downhill simplex runs and scored by their ability to fit experimental data on the temperature dependence of isometric tension and the relationship between force and shortening velocity in frog muscle. We conclude that the first tension-generating step may be weakly endothermic and that the rise of tension with temperature is largely driven by the preceding two strongly endothermic steps of ATP hydrolysis and attachment of M.ADP.Pi to actin. The refined model gave a reasonable fit to the available experimental data and after a temperature jump the overall rate of tension rise was much slower than after a length step as observed experimentally. The findings aid our understanding of the crossbridge cycle by showing that it may not be necessary to include an additional

  5. Shortening Isolation of Patients With Suspected Tuberculosis by Using Polymerase Chain Reaction Analysis

    DEFF Research Database (Denmark)

    Fløe, Andreas; Hilberg, Ole; Thomsen, Vibeke Østergaard;

    2015-01-01

    Background. Isolation of patients suspected for pulmonary tuberculosis is guided by serial sputum smears. This can result in isolation for days for patients with noncontagious tuberculosis. To determine whether a single sample negative for Mycobacterium tuberculosis complex at polymerase chain...... reaction (PCR) can guide isolation. Methods. We retrospectively evaluated sputum samples analyzed for M. tuberculosis complex at the International Reference Laboratory of Mycobacteriology, Copenhagen, Denmark in 2002–2011. We selected culture-confirmed tuberculosis cases with ≥3 samples within 14 days...

  6. Transient shortening strain across an active extensional fault, Basin and Range Province, north-central Nevada, USA, based on geodetic and paleoseismologic data.

    Science.gov (United States)

    Friedrich, A.; Wernicke, B.; Lee, J.; Sieh, K.

    2003-04-01

    The northern Basin and Range province is one of the largest continental extensional regions on earth. At 40 degrees N latitude, the province is 800 km wide and consists of 15 and 20 N-S striking normal faults. These faults accommodated mainly east-west directed extension of tens of kilometers since Mid-Miocene time and recent geodetic surveys show that extension is still active today at a rate of ~1.5 cm/yr across the province (e.g., Bennett et al. 2000; Thatcher et al. 1999). The distribution of this geodetically measurable strain accumulation within the province, however, contradicts geologic observations across some of the active normal faults. For example, coordinated geologic and geodetic measurements across the Crescent Valley fault (CVF), north-central Nevada, reveal a profound mismatch in deformation rates. Since 1996, the two ranges on either side of the CVF have been moving toward each other at ca. 2 mm/yr, indicating shortening. In contrast, new reconnaissance mapping and paleoseismological analyses along the CVF also indicate that this fault is one of the more active normal faults of the Basin and Range province. The 50 km long Cortez Mountains range front is characterized by relief of up to 1.3 km, steep (up to 36 degrees) triangular facets, and young (late Pleistocene to late Holocene) alluvial fans cut by normal fault scarps. Vertical displacement across the CVF is ca. 3 km; since 15 Ma the average long-term vertical displacement rate is ca. 0.2 mm/yr. Topographic profiling shows that fault scarps, 2-7 m high, are the result of a single rupture event and cut late Holocene alluvial fans. A trench across a faulted alluvial fan at Fourmile Canyon reveals a vertical displacement of 4.5 m distributed across two normal faults. 14C analyses on charcoal from a buried offset surface in the hanging wall of the trench and from the base of the overlying colluvial wedge tightly bracket the age of the most recent earthquake to between 2.8 +- 0.1 and 2.7 +- 0.1 ka

  7. Seismic data, geometry, evolution, and shortening in the active Sulaiman fold-and-thrust belt of Pakistan, southwest of the Himalayas

    Energy Technology Data Exchange (ETDEWEB)

    Jadoon, I.A.K. (Quaid-i-Azam Univ., Islamabad (Panama)); Lawrence, R.D.; Lillie, R.J. (Oregon State Univ., Corvallis, OR (United States))

    1994-05-01

    Despite its long history of exploration, the Sulaiman fold and thrust belt is a poorly known structure and detailed structural and geochemical investigations are vital for the successful exploration, evaluation and exploitation of any hydrocarbons. Recent nappe and duplex structural models provide a framework for exploration. Surface and subsurface data from the Sulaiman fold-and-thrust belt are integrated to analyze the deep structure, tectonic, shortening, and kinematics of the Sulaiman fold-and-thrust belt at the western margin of the Indian subcontinent. Seismic reflection data show that nearly all the 10-km-thick sequence of dominantly platform (>7 km) and molasse strata is detached at the deformation front. The strata thicken tectonically to about 20 km in the hinterland without significant thrust faults in the foreland. A balanced structural cross-section suggests that structural uplift in the Sulaiman fold-and-thrust belt is a result of a thin-skinned, passive-roof duplex style of deformation. Sequential restoration of the balanced section reveals a series of structural and geometrical features including: (1) development of low-amplitude, broad concentric folds at the tip of the decollement; (2) increase in amplitude of a detachment fold to a critical level for development of ramp and duplex structures; and (3) out-of-sequence thrusting to create required critical taper for an outward translation of the foreland fold-and-thrust belt. A balanced structural cross-section 349 km long from the Sulaiman fold-and-thrust belt restores to an original length of 727 km, suggesting a maximum of 378 km of shortening since 21 Ma in the cover strata of the Indian subcontinent. Calculation of displacement rates over the Sulaiman fold-and-thrust belt (18 mm/yr) added to the resolved rate of the Chaman fault vector for the component parallel to the plate convergence direction (15 mm/yr) are close to the current India-Asia plate convergence rate (37 mm/yr). 68 refs., 13 figs.

  8. Feasibility of shortening isolation of TB-suspects by first-sample PCR

    DEFF Research Database (Denmark)

    Fløe, Andreas; Wejse, Christian; Thomsen, Vibeke Østergaard

    Rationale: Isolation of patients suspected for tuberculosis (TB) is usually guided by serial sputum smears. Many of patients initially isolated will turn out not to have TB, or will not be regarded as contagious. Current standards imply isolation for hours or days until contagiousness has been......-positive on the sample that produced the PCR-negative result. Conclusion: Though adequate sensitivity in diagnosing TB still requires serial samples for microbiological examination, the question of isolation can be determined by first-sample PCR in the majority of cases, when the test is negative. In our study, less...

  9. NREL's Cyanobacteria Engineering Shortens Biofuel Production Process, Captures CO2

    Energy Technology Data Exchange (ETDEWEB)

    2015-09-01

    This highlight describes NREL's work to systematically analyze the flow of energy in a photosynthetic microbe and show how the organism adjusts its metabolism to meet the increased energy demand for making ethylene. This work successfully demonstrates that the organism could cooperate by stimulating photosynthesis. The results encourage further genetic engineering for the conversion of CO2 to biofuels and chemicals. This highlight is being developed for the September 2015 Alliance S&T Board meeting. biofuels and chemicals. This highlight is being developed for the September 2015 Alliance S&T Board meeting.

  10. Treatment of a Slaughterhouse Wastewater using Sequencing Batch Reactors at a Shortened Operating Cycle

    Directory of Open Access Journals (Sweden)

    Suwadi Saikomon

    2017-01-01

    Full Text Available This laboratory-scale study employed sequencing batch reactor (SBR technology to investigate the effect of two operational parameters [i.e. solids retention time (SRT and anoxic time ratios] regarding the treatment of a slaughterhouse wastewater. Results indicated that organic matter removal, expressed as chemical oxygen demand (COD, was very high, consistently exceeding the 95 % level. In addition, the total nitrogen (TN removal ranged between 82 and 94 %, while total phosphorus (TP removal fluctuated between 88 and 94 %. In general, the reactors exhibited a high degree of operational stability during treatment. Although the investigated range of the two operational parameters appeared to have a minimal effect on the process performance (expressed as % carbon or nutrient removal, the corresponding COD and TN specific consumption rates were noticeably affected by the variation in the anoxic time ratios. Furthermore, the operating cycle length of 8 h employed in this study resulted in improved performance, in terms of nitrogen removal, compared to other studies conducted at longer operating cycles.

  11. [Shortening of the measurement time by 1H-MR turbo spectroscopic imaging of the brain].

    Science.gov (United States)

    Träber, F; Block, W; Lamerichs, R; Keller, E; Schild, H H

    1997-03-01

    Development of a new technique for reduction of measurement time in 1H-MR spectroscopic imaging of the brain. Optimisation of the sequence parameters in volunteer and in patient examinations and comparison to the results obtained with conventional 2 D-SI. Examination of 20 healthy volunteers and 5 patients in a 1.5 T whole-body MR system. In "turbo-spectroscopic imaging" (TSI) sequences, a train of spin-echo signals with different phase encoding is acquired after each 90 degrees excitation. 32 x 32 matrix elements covered a field of view of 20 cm, and additional volume selection was performed by double spin echo excitation. Measurement duration 9 min with acquisition of four phase encoding steps per TR interval, whereas the corresponding 2 D-SI sequence (TR/TE 2000/272 ms) took 30 min. The TSI data sets yielded maps of the regional distribution of metabolite concentrations with a quality comparable to the 2 D-SI results. Signal homogeneity and delineation of brain lesions, however, were superior in conventional spectroscopic imaging. The T2 relaxation of the metabolites required a reduced sampling interval for each phase-encoded echo, and hence the frequency resolution of the corresponding TSI spectra was not always sufficient for separating choline and creatine signals. With measurement durations TSI technique allows in clinical studies a combination with single-voxel MRS for accurate quantification and with a diagnostic MRI within a total examination time of less than one hour.

  12. AMPKalpha1 deletion shortens erythrocyte life span in mice: role of oxidative stress.

    Science.gov (United States)

    Wang, Shaobin; Dale, George L; Song, Ping; Viollet, Benoit; Zou, Ming-Hui

    2010-06-25

    AMP-activated protein kinase (AMPK) is an energy sensor essential for maintaining cellular energy homeostasis. Here, we report that AMPKalpha1 is the predominant isoform of AMPK in murine erythrocytes and mice globally deficient in AMPKalpha1 (AMPKalpha1(-/-)), but not in those lacking AMPKalpha2, and the mice had markedly enlarged spleens with dramatically increased proportions of Ter119-positive erythroid cells. Blood tests revealed significantly decreased erythrocyte and hemoglobin levels with increased reticulocyte counts and elevated plasma erythropoietin concentrations in AMPKalpha1(-/-) mice. The life span of erythrocytes from AMPKalpha1(-/-) mice was less than that in wild-type littermates, and the levels of reactive oxygen species and oxidized proteins were significantly increased in AMPKalpha1(-/-) erythrocytes. In keeping with the elevated oxidative stress, treatment of AMPKalpha1(-/-) mice with the antioxidant, tempol, resulted in decreased reticulocyte counts and improved erythrocyte survival. Furthermore, the expression of Foxo3 and reactive oxygen species scavenging enzymes was significantly decreased in erythroblasts from AMPKalpha1(-/-) mice. Collectively, these results establish an essential role for AMPKalpha1 in regulating oxidative stress and life span in erythrocytes.

  13. 老年性下睑内翻改良矫正术的近期疗效观察%Curative effect of orbicularis muscle shortened with removing eyebag for senile inferior entropion

    Institute of Scientific and Technical Information of China (English)

    廖晓燕

    2015-01-01

    目的:观察联合眼轮匝肌缩短眼袋去除的改良矫正术治疗老年性下睑内翻的近期疗效。方法老年性下睑内翻患者63例(80眼)进行联合眼轮匝肌缩短眼袋去除的改良矫正术,术后随访1年,观察其疗效。结果所有80眼中,1例(1眼)术后出现轻度过矫,其余均达到了良好的矫正效果,1年内无出现复发的病例。结论联合眼轮匝肌缩短眼袋去除的改良矫正术是治疗老年性下睑内翻安全有效的手术矫正方法。%Objective To observe the curative effect of orbicularis muscle shortened with removing eyebag for senile inferior entropion.Methods A total of 63 patients (80 eyes) with senile inferior entropion were treated by orbicularis muscle shortened with removing eyebag, and they were followed up for more than a year.Results At the last follow-up, only 1 patient (1 eye) had a mild postoperative correction, the rest are all achieved good correction results.Within 1 year no relapse cases.Conclusions Orbicularis muscle shortened with removing eyebag seems to be a safe and effective surgical correction method for senile inferior entropion.

  14. In utero TNF-α treatment induces telomere shortening in young adult mice in an ATF7-dependent manner.

    Science.gov (United States)

    Liu, Binbin; Maekawa, Toshio; Chatton, Bruno; Ishii, Shunsuke

    2016-01-01

    Epidemiological studies indicate that exposure to stress during intrauterine life is associated with shorter telomeres in young adulthood, and a correlation between telomere length in early life and lifespan has been suggested. However, empirical studies evaluating these phenomena have not been performed, and the mechanism of stress-induced telomere shortening remains unknown. Since the level of tumour necrosis factor α (TNF-α) in peripheral blood cells is increased by various psychological stresses, the effect of TNF-α administration to pregnant mice on telomere length in adulthood was examined in the present study. In utero TNF-α treatment-induced telomere shortening in adult mice. Telomere shortening was observed in certain tissues such as the bone marrow, spleen, and lung, and was detected at specific age ranges during adulthood. Telomere shortening was not observed in mice lacking the stress-responsive transcription factor ATF7, which contributes to heterochromatin formation in the absence of stress. The present study identified the conditions under which in utero TNF-α treatment induces telomere shortening in adulthood.

  15. Submental Muscle Activity is Delayed and Shortened During Swallowing Following Stroke.

    Science.gov (United States)

    Kim, Hye-Ri; Lee, Seung Ah; Kim, Keewon; Leigh, Ja-Ho; Han, Tai Ryoon; Oh, Byung-Mo

    2015-09-01

    To evaluate the activities of the submental muscles using surface electromyography (sEMG) in patients presenting with dysphagia secondary to middle cerebral artery (MCA) infarction, and to compare the results with those in healthy volunteers. Retrospective study design. A dysphagia clinic at a tertiary care university hospital. Twenty-one patients presenting with dysphagia after unilateral MCA infarction were recruited into the study within 2 months of stroke onset. To serve as a control group, 13 healthy volunteers were enrolled. The primary sEMG parameters were swallowing onset, pretrigger duration, swallowing duration, and swallowing amplitude. Swallowing functional outcome was evaluated using the Videofluoroscopic Dysphagia Scale and the American Speech-Language-Hearing Association National Outcome Measurement System. Patients with MCA infarction demonstrated significantly delayed swallowing onset (P swallowing duration (P = .003) compared with the controls. Duration from the start of sEMG activity and actual laryngeal elevation also was delayed in the patient group (P = .042). At a cut-off point of 0.13 seconds for swallowing onset, sensitivity was 85.7% and specificity was 69.2%. For swallowing duration, sensitivity was 84.6% and specificity was 66.7% with 1.05 seconds as the cut-off point. Patients with MCA infarction showed not only delayed swallowing onset and pretrigger duration, but also shorter duration of sEMG activity compared with those in healthy volunteers. Our results suggest that dysphagia after stroke might be further compromised by delayed laryngeal response especially after the start of suprahyoid muscle activity, which represents impaired laryngeal protection mechanism. Copyright © 2015 American Academy of Physical Medicine and Rehabilitation. Published by Elsevier Inc. All rights reserved.

  16. Shortening start-up and an extension of the power unit load range

    Directory of Open Access Journals (Sweden)

    Taler Jan

    2017-01-01

    Full Text Available A power plant with additional water pressure tanks was proposed. The maximum rise in the block electric power resulting from the shut-off of low-pressure regenerative heaters was determined. At that time, the boiler is fed with hot water from water pressure tanks acting as heat accumulators. Accumulation of hot water in water tanks is also proposed in the periods of the power unit small load. In order to lower the plant electric power in the off-peak night hours, water heated to the nominal temperature in low-pressure regenerative heaters is directed from the feed water tank to pressure tanks. The water accumulated during the night is used to feed the boiler during the period of peak demand for electricity. Pressure accumulators are proposed to be used for the rapid start-up of the boiler from a cold state. The evaporator of the boiler is filled at the beginning of start-up with hot water from the accumulators. Drops in the power block electric power were determined for different capacities of the tanks and periods when they are charged. The tanks may also be used to ensure a sudden increase in the electric power of the unit that is operating in the automatic system of frequency and power control (in Polish: ARCM.

  17. Engineering acyl carrier protein to enhance production of shortened fatty acids.

    Science.gov (United States)

    Liu, Xueliang; Hicks, Wade M; Silver, Pamela A; Way, Jeffrey C

    2016-01-01

    The acyl carrier protein (ACP) is an essential and ubiquitous component of microbial synthesis of fatty acids, the natural precursor to biofuels. Natural fatty acids usually contain long chains of 16 or more carbon atoms. Shorter carbon chains, with increased fuel volatility, are desired for internal combustion engines. Engineering the length specificity of key proteins in fatty acid metabolism, such as ACP, may enable microbial synthesis of these shorter chain fatty acids. We constructed a homology model of the Synechococcus elongatus ACP, showing a hydrophobic pocket harboring the growing acyl chain. Amino acids within the pocket were mutated to increase steric hindrance to the acyl chain. Certain mutant ACPs, when over-expressed in Escherichia coli, increased the proportion of shorter chain lipids; I75 W and I75Y showed the strongest effects. Expression of I75 W and I75Y mutant ACPs also increased production of lauric acid in E. coli that expressed the C12-specific acyl-ACP thioesterase from Cuphea palustris. We engineered the specificity of the ACP, an essential protein of fatty acid metabolism, to alter the E. coli lipid pool and enhance production of medium-chain fatty acids as biofuel precursors. These results indicate that modification of ACP itself could be combined with enzymes affecting length specificity in fatty acid synthesis to enhance production of commodity chemicals based on fatty acids.

  18. Shortened activated partial thromboplastin time, a hemostatic marker for hypercoagulable state during acute coronary event.

    Science.gov (United States)

    Abdullah, Wan Zaidah; Moufak, Shaimaa K; Yusof, Zurkurnai; Mohamad, Mohd Sapawi; Kamarul, I M

    2010-06-01

    Various factors may contribute to a hypercoagulable state and acute vascular thrombosis. A prospective study was conducted involving 165 coronary heart disease (CHD) patients from the Cardiology Unit, Hospital Universiti Sains Malaysia. The purpose of this study was to investigate the relationship among factor VIII (FVIII), prothrombin time (PT), activated partial thromboplastin time (APTT), and activated protein C resistance (APC-R) state among CHD patients and to look for potential clinical applications from these laboratory findings. There were 110 cases diagnosed as acute coronary syndrome (ACS), whereas another 55 were stable coronary artery disease (SCAD) patients. PT, APTT, FVIII, and APC-R assays were performed on all subjects. There was a significant difference between the FVIII level and the APTT results (P value < 0.0001). A negative relationship was found between the FVIII level and the APTT from linear regression analysis (R(2) = 10%, P value < 0.0001). For each 1% increase in the FVIII level, the APTT was reduced by 0.013 s (95% confidence interval (CI) between -0.019 and -0.007). Interestingly, none of the SCAD patients had abnormally short APTT. Approximately 68.4% of cases with a positive APC-R assay were found to have a high FVIII level. In conclusion, the APTT test is a potential hemostatic marker for hypercoagulable state including in arterial thrombosis.

  19. Muscle shortening velocity depends on tissue inertia and level of activation during submaximal contractions.

    Science.gov (United States)

    Ross, Stephanie A; Wakeling, James M

    2016-06-01

    In order to perform external work, muscles must do additional internal work to deform their tissue, and in particular, to overcome the inertia due to their internal mass. However, the contribution of the internal mass within a muscle to the mechanical output of that muscle has only rarely been studied. Here, we use a dynamic, multi-element Hill-type muscle model to examine the effects of the inertial mass within muscle on its contractile performance. We find that the maximum strain-rate of muscle is slower for lower activations and larger muscle sizes. As muscle size increases, the ability of the muscle to overcome its inertial load will decrease, as muscle tension is proportional to cross-sectional area and inertial load is proportional to mass. Thus, muscles that are larger in size will have a higher inertial cost to contraction. Similarly, when muscle size and inertial load are held constant, decreasing muscle activation will increase inertial cost to contraction by reducing muscle tension. These results show that inertial loads within muscle contribute to a slowing of muscle contractile velocities (strain-rates), particularly at the submaximal activations that are typical during animal locomotion.

  20. Shortened Time to Identify Staphylococcus Species from Blood Cultures and Methicillin Resistance Testing Using CHROMAgar

    Directory of Open Access Journals (Sweden)

    Shingo Chihara

    2009-01-01

    Full Text Available The ability to rapidly differentiate coagulase-negative staphylococcus (CoNS from Staphylococcus aureus and to determine methicillin resistance is important as it affects the decision to treat empiric antibiotic selection. The objective of this study was to evaluate CHROMagar S. aureus and CHROMagar MRSA (Becton Dickinson for rapid identification of Staphylococcus spp. directly from blood cultures. Consecutive blood culture bottles (BacT Alert 3D SA and SN, bioMérieux growing gram-positive cocci in clusters were evaluated. An aliquot was plated onto CHROMagar MRSA (C-MRSA and CHROMagar S. aureus (C-SA plates, which were read at 12 to 16 hours. C-SA correctly identified 147/147 S. aureus (100% sensitivity; 2 CoNS were misidentified as S. aureus (98% specificity. C-MRSA correctly identified 74/77 MRSA (96% sensitivity. None of the MSSA isolates grew on C-MRSA (100% specificity. In conclusion, CHROMagar is a rapid and sensitive method to distinguish MRSA, MSSA, and coagulase-negative Staphylococcus and may decrease time of reporting positive results.

  1. New GES DISC Services Shortening the Path in Science Data Discovery

    Science.gov (United States)

    Li, Angela; Shie, Chung-Lin; Petrenko, Maksym; Hegde, Mahabaleshwa; Teng, William; Liu, Zhong; Bryant, Keith; Shen, Suhung; Hearty, Thomas; Wei, Jennifer; Pham, Long

    2017-01-01

    The Current GES DISC available services only allow user to select variables from a single dataset at a time and too many variables from a dataset are displayed, choice is hard. At American Geophysical Union (AGU) 2016 Fall Meeting, Goddard Earth Sciences Data Information Services Center (GES DISC) unveiled a new service: Datalist. A Datalist is a collection of predefined or user-defined data variables from one or more archived datasets. Our science support team curated predefined datalist and provided value to the user community. Imagine some novice user wants to study hurricane and typed in hurricane in the search box. The first item in the search result is GES DISC provided Hurricane Datalist. It contains scientists recommended variables from multiple datasets like TRMM, GPM, MERRA, etc. Datalist uses the same architecture as that of our new website, which also provides one-stop shopping for data, metadata, citation, documentation, visualization and other available services.We implemented Datalist with new GES DISC web architecture, one single web page that unified all user interfaces. From that webpage, users can find data by either type in keyword, or browse by category. It also provides user with a sophisticated integrated data and services package, including metadata, citation, documentation, visualization, and data-specific services, all available from one-stop shopping.

  2. Direct look from a predator shortens the risk-assessment time by prey.

    Directory of Open Access Journals (Sweden)

    Sang-im Lee

    Full Text Available Decision making process is an important component of information use by animals and has already been studied in natural situations. Decision making takes time, which is expressed as a cost in evolutionary explanations of decision making abilities of animals. However, the duration of information assessment and decision making process has not been measured in a natural situation. Here, we use responses of wild magpies (Pica pica to predictably approaching humans to demonstrate that, regardless of whether the bird perceived high (decided to fly away or low (resumed foraging threat level, the bird assessed the situation faster when approaching humans looked directly at it than when the humans were not directly looking at it. This indicates that prey is able to extract more information about the predator's intentions and to respond sooner when the predator is continuously ("intently" looking at the prey. The results generally illustrate how an increase of information available to an individual leads to a shorter assessment and decision making process, confirming one of central tenets of psychology of information use in a wild bird species in its natural habitat.

  3. Neuro-mechanical adjustments to shod versus barefoot treadmill runs in the acute and delayed stretch-shortening cycle recovery phases.

    Science.gov (United States)

    Morio, Cedric; Sevrez, Violaine; Chavet, Pascale; Berton, Eric; Nicol, Caroline

    2016-01-01

    In habitually shod recreational runners, we studied the combined influence of footwear and stretch-shortening cycle (SSC) fatigue on treadmill running pattern, paying special attention to neuro-mechanical adjustments in the acute and 2-day delayed recovery periods. The SSC exercise consisted of a series of 25 sub-maximal rebounds on a sledge apparatus repeated until exhaustion. The acute and delayed functional fatigue effects were quantified in a maximal drop jump test. The neuro-mechanical adjustments to fatigue were examined during two submaximal treadmill run tests of 3 min performed either barefoot or with shoes on. Surface electromyographic (EMG) activities, tibial accelerations and kinematics of the right lower limb were recorded during the first and last 15 s of each run. The main result was that neuro-mechanical differences between the shod and barefoot running patterns, classically reported in the absence of fatigue, persisted in the fatigued state. However, in the delayed recovery phase, rearfoot eversion was found to significantly increase in the shod condition. This specific footwear effect is considered as a potential risk factor of overuse injuries in longer runs. Therefore, specific care should be addressed in the delayed recovery phase of SSC fatigue and the use of motion control shoes could be of interest.

  4. A chopper system for shortening the duration of pulsed supersonic beams seeded with NO or Br2 down to 13 microseconds

    CERN Document Server

    Lam, Jessica; Softley, Tim

    2015-01-01

    A chopper wheel construct is used to shorten the duration of a molecular beam to 13 microseconds. Molecular beams seeded with NO or with Br2 and an initial pulse width of greater or equal to 200 microseconds were passed through a spinning chopper wheel, which was driven by a brushless DC in vacuo motor at a range of speeds, from 3,000 rpm to 80,000 rpm. The resulting duration of the molecular-beam pulses measured at the laser detection volume ranged from 80 microseconds to 13 microseconds, and was the same for both NO and Br2. The duration is consistent with a simple analytical model, and the minimum pulse width measured is limited by the spreading of the beam between the chopper and the detection point as a consequence of the longitudinal velocity distribution of the beam. The setup adopted here effectively eliminates buildup of background gas without the use of a differential pumping stage, and a clean narrow pulse is obtained with low rotational temperature.

  5. A chopper system for shortening the duration of pulsed supersonic beams seeded with NO or Br2 down to 13 μs

    Science.gov (United States)

    Lam, Jessica; Rennick, Christopher J.; Softley, Timothy P.

    2015-05-01

    A chopper wheel construct is used to shorten the duration of a molecular beam to 13 μs. Molecular beams seeded with NO or with Br2 and an initial pulse width of ≥200 μs were passed through a spinning chopper wheel, which was driven by a brushless DC in vacuo motor at a range of speeds, from 3000 rpm to 80 000 rpm. The resulting duration of the molecular-beam pulses measured at the laser detection volume ranged from 80 μs to 13 μs and was the same for both NO and Br2. The duration is consistent with a simple analytical model, and the minimum pulse width measured is limited by the spreading of the beam between the chopper and the detection point as a consequence of the longitudinal velocity distribution of the beam. The setup adopted here effectively eliminates buildup of background gas without the use of a differential pumping stage, and a clean narrow pulse is obtained with low rotational temperature.

  6. A chopper system for shortening the duration of pulsed supersonic beams seeded with NO or Br{sub 2} down to 13 μs

    Energy Technology Data Exchange (ETDEWEB)

    Lam, Jessica; Rennick, Christopher J.; Softley, Timothy P. [Department of Chemistry, University of Oxford, Chemistry Research Laboratory, 12 Mansfield Rd, Oxford OX1 3TA (United Kingdom)

    2015-05-15

    A chopper wheel construct is used to shorten the duration of a molecular beam to 13 μs. Molecular beams seeded with NO or with Br{sub 2} and an initial pulse width of ≥200 μs were passed through a spinning chopper wheel, which was driven by a brushless DC in vacuo motor at a range of speeds, from 3000 rpm to 80 000 rpm. The resulting duration of the molecular-beam pulses measured at the laser detection volume ranged from 80 μs to 13 μs and was the same for both NO and Br{sub 2}. The duration is consistent with a simple analytical model, and the minimum pulse width measured is limited by the spreading of the beam between the chopper and the detection point as a consequence of the longitudinal velocity distribution of the beam. The setup adopted here effectively eliminates buildup of background gas without the use of a differential pumping stage, and a clean narrow pulse is obtained with low rotational temperature.

  7. Acute and 2 days delayed effects of exhaustive stretch-shortening cycle exercise on barefoot walking and running patterns.

    Science.gov (United States)

    Morio, Cédric; Nicol, Caroline; Barla, Charlie; Barthèlemy, Joëlle; Berton, Eric

    2012-08-01

    This study investigated the acute and 2 days delayed influences of exhaustive stretch-shortening cycle exercise (SSC) on barefoot walking and running gait patterns. The SSC exercise was performed on a sledge apparatus, on which the subjects (N = 10) repeated until exhaustion intermittent series of 25 bilateral submaximal rebounds. Maximal drop-jumps and submaximal barefoot treadmill walking and running were performed before (PRE) and after (POST) the exhaustive exercise and repeated 48 h (D2) later. Electromyographic activity and 3D kinematics of the right lower limb and foot were recorded for 15 s at gait initiation (BEG) and at the end (END: at 3 min of walk and 5 min of run). The exhaustive SSC exercise resulted in 6% reductions in maximal drop jump performance at POST and D2, and affected mostly both gait patterns at D2. The walking pattern presented compensatory neural adjustments within the triceps surae muscle group. This expected pain-induced protective strategy of the soleus muscle was sufficient to preserve the kinematics pattern. The running condition revealed a major knee strategy, which might support the concept of pain protective strategy of knee extensor muscles at the expense of impact cushioning. Regardless the testing session, most parameters showed fatigue-induced changes at gait initiation (BEG), which were opposite to subsequent BEG to END adjustments. This is likely to support anticipatory strategies rather than progressive adjustments during the exercise.

  8. Blockade by NIP-142, an antiarrhythmic agent, of carbachol-induced atrial action potential shortening and GIRK1/4 channel.

    Science.gov (United States)

    Matsuda, Tomoyuki; Ito, Mie; Ishimaru, Sayoko; Tsuruoka, Noriko; Saito, Tomoaki; Iida-Tanaka, Naoko; Hashimoto, Norio; Yamashita, Toru; Tsuruzoe, Nobutomo; Tanaka, Hikaru; Shigenobu, Koki

    2006-08-01

    Mechanisms for the atria-specific action potential-prolonging action of NIP-142 ((3R*,4S*)-4-cyclopropylamino-3,4-dihydro-2,2-dimethyl-6-(4-methoxyphenylacetylamino)-7-nitro-2H-1-benzopyran-3-ol), a benzopyran compound that terminates experimental atrial arrhythmia, was examined. In isolated guinea-pig atrial tissue, NIP-142 reversed the shortening of action potential duration induced by either carbachol or adenosine. These effects were mimicked by tertiapin, but not by E-4031. NIP-142 concentration-dependently blocked the human G protein-coupled inwardly rectifying potassium channel current (GIRK1/4 channel current) expressed in HEK-293 cells with an EC50 value of 0.64 microM. At higher concentrations, NIP-142 blocked the human ether a go-go related gene (HERG) channel current with an EC50 value of 44 microM. In isolated guinea-pig papillary muscles, NIP-142 had no effect on the negative inotropic effect of carbachol under beta-adrenergic stimulation, indicating lack of effect on the muscarinic receptor and Gi protein. These results suggest that NIP-142 directly inhibits the acetylcholine-activated potassium current.

  9. Surgical Treatment of an Infected Nonunion of the Middle Third of the Femur Associated with Femoral Shortening in a Hemophilia Patient

    Directory of Open Access Journals (Sweden)

    Ahmet Salduz

    2016-01-01

    Full Text Available The management of nonunion and limb length discrepancy has remained a constant challenge in hemophilic patients. In this study, we aimed to present the treatment of femur infected nonunion and limb length discrepancy in a twenty-seven-year-old patient with hemophilia type A. A 27-year-old male patient with hemophilia type A referred to our institution for the treatment of right femur infected nonunion and 10 cm shortness of the femur. Resection of the nonunion site and bone-to-bone fixation with autologous bone grafting were performed. Compression to the pseudoarthrosis site and distraction from new osteotomy site were applied with the unilateral external fixator. Union was achieved, and 6 cm lengthening was obtained according to the initial length. Patient was followed up for 7 years. After this treatment, the patient is able to walk with full weight bearing on the affected extremity with 4 cm shortening which is compensated by the heel lift. The results of this case indicate that limb lengthening and treatment of nonunion with the external fixation could be reliable and effective method for hemophilic patients.

  10. Percutaneous pelvic osteotomy and intertrochanteric varus shortening osteotomy in nonambulatory GMFCS level IV and V cerebral palsy patients: preliminary report on 30 operated hips.

    Science.gov (United States)

    Canavese, Federico; Gomez, Horacio; Kaelin, André; Ceroni, Dimitri; de Coulon, Geraldo

    2013-01-01

    This study evaluated the outcome of severe cerebral palsy patients (Gross Motor Function Classification System level IV and V) treated by simultaneous percutaneous pelvic osteotomy and intertrochanteric varus shortening osteotomy for hip subluxation or dislocation between 2002 and 2011. Twenty-four patients (30 hips) with an average age of 9.4 years (5-16.5) were reviewed at a mean follow-up of 35.9 months (6-96). Percutaneous pelvic osteotomy lasted on average 30 min/patient per side (25-40) and was always performed through a skin incision of 2-3 cm. The migration percentage and acetabular angle were assessed on plain radiographs. The mean Reimers' migration percentage improved from 67.1% (42-100) preoperatively to 7.7% (0-70) at the last follow-up and the mean acetabular angle improved from 31.8° (22-48) to 15.7° (5-27). Five patients presented complications: one redislocation, one bone graft dislodgement, and three with avascular necrosis of the femoral head. This study should be considered as a pilot study. These results indicate that this combined approach is an effective, reliable, and minimally invasive alternative method for the treatment of spastic dislocated hips in severe cerebral palsy patients with an outcome similar to standard techniques reported in the literature.

  11. Crustal shortening, exhumation, and strain localization in a collisional orogen: The Bajo Pequeño Shear Zone, Sierra de Pie de Palo, Argentina

    Science.gov (United States)

    Garber, Joshua M.; Roeske, Sarah M.; Warren, Jessica; Mulcahy, Sean R.; McClelland, William C.; Austin, Lauren J.; Renne, Paul R.; Vujovich, Graciela I.

    2014-07-01

    The Bajo Pequeño Shear Zone (BPSZ) is a lower-crustal shear zone that records shortening and exhumation associated with the establishment of a new plate boundary, and its placement in a regional structural context suggests that local- to regional-scale strain localization occurred with progressive deformation. A kilometer-scale field and analytical cross section through the ~80 m thick BPSZ and its adjacent rocks indicates an early Devonian (405-400 Ma) phase of deformation on the western margin of Gondwanan continental crust. The earliest stages of the BPSZ, recorded by metamorphic and microstructural data, involved thrusting of a hotter orthogneiss over a relatively cool pelitic unit, which resulted in footwall garnet growth and reset footwall white mica 40Ar/39Ar ages in proximity to the shear zone. Later stages of BPSZ activity, as recorded by additional microstructures and quartz c-axis opening angles, were characterized by strain localization to the center of the shear zone coincident with cooling and exhumation. These and other data suggest that significant regional tectonism persisted in the Famatinian orogenic system for 60-70 million years after one microplate collision (the Precordillera) but ceased 5-10 million years prior to another (Chilenia). A survey of other synchronous structures shows that strain was accommodated on progressively narrower structures with time, indicating a regional pattern of strain localization and broad thermal relaxation as the Precordillera collision evolved.

  12. Shortening baroreflex delay in hypertrophic cardiomyopathy patients – an unknown effect of β-blockers

    Science.gov (United States)

    Katarzynska-Szymanska, Agnieszka; Ochotny, Romuald; Oko-Sarnowska, Zofia; Wachowiak-Baszynska, Hanna; Krauze, Tomasz; Piskorski, Jaroslaw; Gwizdala, Adrian; Mitkowski, Przemyslaw; Guzik, Przemyslaw

    2013-01-01

    Aims Hypertrophic cardiomyopathy (HCM) is characterized by left ventricular hypertrophy and impaired diastolic and systolic function. Abnormal sympathetic–parasympathetic balance is a potential stimulus for left ventricular hypertrophy in HCM patients. β-Blockers are routinely used in HCM for their strong negative inotropic effect; however, these drugs also influence the sympathetic–parasympathetic balance. This study aimed to determine the autonomic control of the cardiovascular system and the autonomic effects of β-blockers in HCM patients treated or untreated with β-blockers. Methods Among 51 HCM outpatients (18–70 years old; 29 men) there were 19 individuals with no medication and 32 subjects treated with a β-blocker. Fourteen age- and gender-matched (23–70 years old; nine men) healthy volunteers were enrolled in the control group. Continuous, non-invasive finger blood pressure was recorded during supine rest for 30 min. Autonomic regulation of the cardiovascular system was measured by heart rate variability and spontaneous baroreflex function (cross-correlation sequence method). Results The mean pulse interval, time domain and spectral measures of heart rate variability and baroreflex sensitivity were comparable between HCM patients, treated or not with β-blockers, and the control group. However, the delay of the baroreflex was significantly longer in HCM patients who were not treated with β-blockers [2.0 (1.6–2.3) s] in comparison with HCM patients receiving β-blockers [1.4 (1.1–1.8) s; P = 0.0072] or control subjects [1.2 (0.8–1.8) s; P = 0.0025]. This delay did not differ between HCM patients treated with β-blockers and the control group. Conclusions Hypertrophic cardiomyopathy not treated with β-blockers is accompanied by prolonged baroreflex delay. The use of β-blockers normalizes this delay. PMID:23126403

  13. Shortening a survey and using alternative forms of prenotification: Impact on response rate and quality

    Directory of Open Access Journals (Sweden)

    Jenkins Sarah

    2010-06-01

    Full Text Available Abstract Background Evidence suggests that survey response rates are decreasing and that the level of survey response can be influenced by questionnaire length and the use of pre-notification. The goal of the present investigation was determine the effect of questionnaire length and pre-notification type (letter vs. postcard on measures of survey quality, including response rates, response times (days to return the survey, and item nonresponse. Methods In July 2008, the authors randomized 900 residents of Olmsted County, Minnesota aged 25-65 years to one of two versions of the Talley Bowel Disease Questionnaire, a survey designed to assess the prevalence of functional gastrointestinal disorders (FGID. One version was two pages long and the other 4 pages. Using a 2 × 2 factorial design, respondents were randomized to survey length and one of two pre-notification types, letter or postcard; 780 residents ultimately received a survey, after excluding those who had moved outside the county or passed away. Results Overall, the response rates (RR did not vary by length of survey (RR = 44.6% for the 2-page survey and 48.4% for the 4-page or pre-notification type (RR = 46.3% for the letter and 46.8% for the postcard. Differences in response rates by questionnaire length were seen among younger adults who were more likely to respond to the 4-page than the 2-page questionnaire (RR = 39.0% compared to 21.8% for individuals in their 20s and RR = 49.0% compared to 32.3% for those in their 30s. There were no differences across conditions with respect to item non-response or time (days after mailing to survey response. Conclusion This study suggests that the shortest survey does not necessarily provide the best option for increased response rates and survey quality. Pre-notification type (letter or postcard did not impact response rate suggesting that postcards may be more beneficial due to the lower associated costs of this method of contact.

  14. Increased Eligibility for Treatment of Chronic Hepatitis C Infection with Shortened Duration of Therapy: Implications for Access to Care and Elimination Strategies in Canada

    Directory of Open Access Journals (Sweden)

    Sergio M Borgia

    2015-01-01

    Full Text Available BACKGROUND: All oral, highly effective direct-acting antiviral combinations, such as sofosbuvir-ledipasvir, have recently been licensed in Canada but cost as much as $67,000 for a 12-week course of therapy, representing a major economic barrier to predominately single-payer health care systems such as that found in Ontario. In hepatitis C virus (HCV genotype 1 noncirrhotic patients with a baseline viral load of <6×106 IU/mL, treatment with sofosbuvir-ledipasvir can be shortened to eight weeks without compromising ≥95% efficacy. The number of HCV-infected patients in Ontario eligible for shortened therapy, and the associated cost savings, are unknown. The authors propose that treating every patient with shortened therapy, regardless of baseline viral load, would lead to significant public cost savings and collateral efficiencies, enabling increased HCV treatment capacity and cure.

  15. Successful shortening of tuberculosis treatment using adjuvant host-directed therapy with FDA-approved phosphodiesterase inhibitors in the mouse model.

    Directory of Open Access Journals (Sweden)

    Mamoudou Maiga

    Full Text Available Global control of tuberculosis (TB, an infectious disease that claims nearly 2 million lives annually, is hindered by the long duration of chemotherapy required for curative treatment. Lack of adherence to this intense treatment regimen leads to poor patient outcomes, development of new or additional drug resistance, and continued spread of M.tb. within communities. Hence, shortening the duration of TB therapy could increase drug adherence and cure in TB patients. Here, we report that addition of the United Stated Food and Drug Administration-approved phosphodiesterase inhibitors (PDE-Is cilostazol and sildenafil to the standard TB treatment regimen reduces tissue pathology, leads to faster bacterial clearance and shortens the time to lung sterilization by one month, compared to standard treatment alone, in a murine model of TB. Our data suggest that these PDE-Is could be repurposed for use as adjunctive drugs to shorten TB treatment in humans.

  16. Successful Shortening of Tuberculosis Treatment Using Adjuvant Host-Directed Therapy with FDA-Approved Phosphodiesterase Inhibitors in the Mouse Model

    Science.gov (United States)

    Ammerman, Nicole C.; Gupta, Radhika; Guo, Haidan; Maiga, Marama C.; Lun, Shichun; Bishai, William R.

    2012-01-01

    Global control of tuberculosis (TB), an infectious disease that claims nearly 2 million lives annually, is hindered by the long duration of chemotherapy required for curative treatment. Lack of adherence to this intense treatment regimen leads to poor patient outcomes, development of new or additional drug resistance, and continued spread of M.tb. within communities. Hence, shortening the duration of TB therapy could increase drug adherence and cure in TB patients. Here, we report that addition of the United Stated Food and Drug Administration-approved phosphodiesterase inhibitors (PDE-Is) cilostazol and sildenafil to the standard TB treatment regimen reduces tissue pathology, leads to faster bacterial clearance and shortens the time to lung sterilization by one month, compared to standard treatment alone, in a murine model of TB. Our data suggest that these PDE-Is could be repurposed for use as adjunctive drugs to shorten TB treatment in humans. PMID:22319585

  17. Fatty acid composition and contents of trans monounsaturated fatty acids in frying fats, and in margarines and shortenings marketed in Denmark

    DEFF Research Database (Denmark)

    Ovesen, L.; Leth, Torben; Hansen, K.

    1998-01-01

    of trans long-chain fatty acids. Both fast-food frying fats contained large amounts of trans C-18:1, 21.9 +/- 2.9% in Burger King and 16.6 +/- 0.4% in McDonald's. In Denmark the per capita supply of trans C-18:1 from margarines and shortenings and frying fats has decreased steadily during recent years....... The supply of trans C-18:1 from margarines and shortenings in the Danish diet is now 1.1 g per day....

  18. Age- and gender-related development of stretch shortening cycle during a sub-maximal hopping task

    Directory of Open Access Journals (Sweden)

    G Laffaye

    2016-01-01

    Full Text Available The aim of this study was to analyse the effects of age and gender (and their interaction on a stretch shortening cycle solicited during a hopping task. For this aim, 147 girls and 148 boys aged 11 to 20 years, who were enrolled in middle school or secondary school with no experience in sport activity, or training less than three times per week, performed 3x5 hops in place. Leg-stiffness, jump-height and reactive-strength indices were assessed using an accelerometer (Myotest. The participants were selected in order to form five age groups: 11 12, 13-14, 15-16, 17-18 and 19-20 years. Regression analysis between force and centre of mass displacement revealed spring-mass behavior for all groups (r2=73-89, meaning that beginning at the age of 11 years, children are able to perform complex inter-muscular coordination of the lower limbs, revealing efficient neural control early in childhood. Leg stiffness increased from 24.7 ± 10.6 kN • m-1 at 11-12 years to 44.1 ± 14 kN • m-1 in boys, with a small increase until 16 years (+17% and a large increase between 17 and 20 years (+32.7%. In girls, leg stiffness increased from 26.6 ± 9 kN • m-1 at 11-12 years to 39.4 ± 10.9 kN • m-1 at 19-20 years, with a curious decrease in leg stiffness at 17-18 years, probably due to an increase in the percentage of fat at this age (25%. While no gender effect was found, the reactive-strength index revealed that, from 15-16 years onward, boys were better able to produce high levels of force in a shorter time than girls. The age of 15-16 years is a threshold of maturity and gender differentiation, where the boys investigated are more efficient in the stretch shortening cycle.

  19. Leg lengthening and shortening

    Science.gov (United States)

    ... epiphysiodesis), which may cause short height Bone infection ( osteomyelitis ) Injury to blood vessels Poor bone healing Nerve ... the skin color, temperature, and sensation of the foot and toes frequently. This will help find any ...

  20. Recent rapid shortening of crust across the Tianshan Mts. and relative motion of tectonic blocks in the north and south

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    Based on the multiple-epoch Global Positioning System observations during a period from 1992 to 1999, we document directly a rapid crustal shortening of ~20 mm/a across the western Tianshan Mts. (76°E), in contrast to a 4 mm/a convergent rate across the eastern Tianshan Mts. (87°E)and the north-south convergence across the mountain belt descends laterally from west to east. The direction of current crustal movement inferred by GPS sites along the southern flank of the Tianshan Mts. is approximately perpendicular to the easterly-trending mountain belt, indicating that the Tarim Basin thrust almost rightly into the Tianshan Mts. The Tarim Basin accommodates nearly no or a minor, if any, crustal deformation and rotates clockwise, as a rigid body in a whole, at a rate of 0.64°/Ma around a Euler pole at 95.7°E, 40.3°N (Anxi, Gansu) with respect to the stable Siberia. The relative motion between the Kazakh platform and the Dzungarian Basin is quite apparent. The Dzungar should be regarded as an independent active block from the view of the Asia tectonic settings.

  1. Temporal, but not directional, prior knowledge shortens muscle reflex latency in response to sudden transition of support surface during walking

    Directory of Open Access Journals (Sweden)

    Masahiro eShinya

    2016-02-01

    Full Text Available The central nervous system takes advantage of prior knowledge about potential upcoming perturbations for modulating postural reflexes. There are two distinct aspects of prior knowledge: spatial and temporal. This study investigated how each of spatial and temporal prior knowledge contributes to the shortening of muscle response latency. Eleven participants walked on a split-belt treadmill and perturbed by sudden acceleration or deceleration of the right belt at right foot contact. Spatial prior knowledge was given by instruction of possible direction (e.g., only acceleration of upcoming perturbation at the beginning of an experimental session. Temporal prior knowledge was given to subjects by warning tones at foot contact during three consecutive strides before the perturbation. In response to acceleration perturbation, reflexive muscle activity was observed in soleus and gastrocnemius muscles. Onset latency of the gastrocnemius response was shorter (72 ms vs. 58 ms when subjects knew the timing of the upcoming perturbation, whereas the latency was independent of directional prior knowledge. Soleus onset latency (44 ms was not influenced by directional nor temporal prior knowledge. Although spinal neural circuit that mediates short-latency relfex was not influenced by the prior knowledge, excitability in supra-spinal neural circuit that mediates medium- and long-latency reflex might be enhanced by knowing the timing of the upcoming perturbation.

  2. Monolayer cultivation of osteoprogenitors shortens duration of the embryonic stem cell test while reliably predicting developmental osteotoxicity.

    Science.gov (United States)

    zur Nieden, Nicole I; Davis, Lesley A; Rancourt, Derrick E

    2010-11-09

    Osteotoxic compounds administered during pregnancy can initiate skeletal congenital anomalies in the embryo. In vitro, developmental osteotoxicity of a compound can be predicted with the embryonic stem cell test (EST), the only in vitro embryotoxicity model identified to date that entirely abrogates the use of animals. Although the previously identified endpoint osteocalcin mRNA expression robustly predicts developmental osteotoxicity, it can only be assayed after 5 weeks of in vitro culture with existing embryoid body (EB)-based differentiation protocols. Therefore, the goal of this study was to characterize novel earlier endpoints of developmental osteotoxicity for the EST. The currently used EB-based differentiation protocol was modified so that a monolayer culture of pre-differentiated cells was inoculated. The expression profile of five bone-specific mRNAs, including osteocalcin, over the course of 30 differentiation days suggested an acceleration of pre-osteoblast specification in the monolayer over the EB-based protocol. Similarly, calcification was already visible after 14 days of culture in monolayer cultures. Employing image and absorption-based techniques to measure the degree of mineralization in these cells after compound treatment, the three compounds Penicillin G, 5-fluorouracil (5-FU) and all-trans retinoic acid (RA) were then tested after 14 days in monolayer cultures and compared to embryoid body-based differentiations at day 30. By modifying the culture the three test substances were classified correctly into non- or strong osteotoxic. Moreover, we were successful in shortening the assay duration from 30 to 14 days.

  3. Crustal structure of the Bighorn Mountains region: Precambrian influence on Laramide shortening and uplift in north-central Wyoming

    Science.gov (United States)

    Worthington, Lindsay L.; Miller, Kate C.; Erslev, Eric A.; Anderson, Megan L.; Chamberlain, Kevin R.; Sheehan, Anne F.; Yeck, William L.; Harder, Steven H.; Siddoway, Christine S.

    2016-01-01

    The crustal structure of north-central Wyoming records a history of complex lithospheric evolution from Precambrian accretion to Cretaceous-Paleogene Laramide shortening. We present two active source P wave velocity model profiles collected as part of the Bighorn Arch Seismic Experiment in 2010. Analyses of these velocity models and single-fold reflection data, together with potential field modeling of regional gravity and magnetic signals, constrain crustal structure and thickness of the Bighorn region. We image a west dipping reflection boundary and model a sharp magnetic contact east of the Bighorn Arch that together may delineate a previously undetected Precambrian suture zone. Localized patches of a high-velocity, high-density lower crustal layer (the "7.× layer") occur across the study area but are largely absent beneath the Bighorn Arch culmination. Moho topography is relatively smooth with no large-scale offsets, with depths ranging from ~50 to 37 km, and is largely decoupled from Laramide basement topography. These observations suggest that (1) the edge of the Archean Wyoming craton lies just east of the Bighorn Mountains, approximately 300 km west of previous interpretations, and (2) Laramide deformation localized in an area with thin or absent 7.× layer, due to its relatively weak lower crust, leading to detachment faulting. Our findings show that Precambrian tectonics in northern Wyoming may be more complicated than previously determined and subsequent Laramide deformation may have been critically dependent on laterally heterogeneous crustal structure that can be linked to Precambrian origins.

  4. Comparison of different cooling regimes within a shortened liquid cooling/warming garment on physiological and psychological comfort during exercise

    Science.gov (United States)

    Leon, Gloria R.; Koscheyev, Victor S.; Coca, Aitor; List, Nathan

    2004-01-01

    The aim of this study was to compare the effectiveness of different cooling regime intensities to maintain physiological and subjective comfort during physical exertion levels comparable to that engaged in during extravehicular activities (EVA) in space. We studied eight subjects (six males, two females) donned in our newly developed physiologically based shortened liquid cooling/warming garment (SLCWG). Rigorous (condition 1) and mild (condition 2) water temperature cooling regimes were compared at physical exertion levels comparable to that performed during EVA to ascertain the effectiveness of a lesser intensity of cooling in maintaining thermal comfort, thus reducing energy consumption in the portable life support system. Exercise intensity was varied across stages of the session. Finger temperature, rectal temperature, and subjective perception of overall body and hand comfort were assessed. Finger temperature was significantly higher in the rigorous cooling condition and showed a consistent increase across exercise stages, likely due to the restriction of heat extraction because of the intensive cold. In the mild cooling condition, finger temperature exhibited an overall decline with cooling, indicating greater heat extraction from the body. Rectal temperature was not significantly different between conditions, and showed a steady increase over exercise stages in both rigorous and mild cooling conditions. Ratings of overall comfort were 30% higher (more positive) and more stable in mild cooling (p<0.001). The mild cooling regime was more effective than rigorous cooling in allowing the process of heat exchange to occur, thus maintaining thermal homeostasis and subjective comfort during physical exertion.

  5. PEP3 overexpression shortens lag phase but does not alter growth rate in Saccharomyces cerevisiae exposed to acetic acid stress.

    Science.gov (United States)

    Ding, Jun; Holzwarth, Garrett; Bradford, C Samuel; Cooley, Ben; Yoshinaga, Allen S; Patton-Vogt, Jana; Abeliovich, Hagai; Penner, Michael H; Bakalinsky, Alan T

    2015-10-01

    In fungi, two recognized mechanisms contribute to pH homeostasis: the plasma membrane proton-pumping ATPase that exports excess protons and the vacuolar proton-pumping ATPase (V-ATPase) that mediates vacuolar proton uptake. Here, we report that overexpression of PEP3 which encodes a component of the HOPS and CORVET complexes involved in vacuolar biogenesis, shortened lag phase in Saccharomyces cerevisiae exposed to acetic acid stress. By confocal microscopy, PEP3-overexpressing cells stained with the vacuolar membrane-specific dye, FM4-64 had more fragmented vacuoles than the wild-type control. The stained overexpression mutant was also found to exhibit about 3.6-fold more FM4-64 fluorescence than the wild-type control as determined by flow cytometry. While the vacuolar pH of the wild-type strain grown in the presence of 80 mM acetic acid was significantly higher than in the absence of added acid, no significant difference was observed in vacuolar pH of the overexpression strain grown either in the presence or absence of 80 mM acetic acid. Based on an indirect growth assay, the PEP3-overexpression strain exhibited higher V-ATPase activity. We hypothesize that PEP3 overexpression provides protection from acid stress by increasing vacuolar surface area and V-ATPase activity and, hence, proton-sequestering capacity.

  6. PEP3 overexpression shortens lag phase but does not alter growth rate in Saccharomyces cerevisiae exposed to acetic acid stress

    Science.gov (United States)

    Ding, Jun; Holzwarth, Garrett; Bradford, C. Samuel; Cooley, Ben; Yoshinaga, Allen S.; Patton-Vogt, Jana; Abeliovich, Hagai; Penner, Michael H.; Bakalinsky, Alan T.

    2017-01-01

    In fungi, two recognized mechanisms contribute to pH homeostasis: the plasma membrane proton-pumping ATPase that exports excess protons and the vacuolar proton-pumping ATPase (V-ATPase) that mediates vacuolar proton uptake. Here, we report that overexpression of PEP3 which encodes a component of the HOPS and CORVET co