WorldWideScience

Sample records for vulgaris carrying bc-3

  1. Potyviral resistance derived from cultivars of Phaseolus vulgaris carrying bc-3 co-segregates with homozygotic presence of a mutated eIF4E allele

    DEFF Research Database (Denmark)

    Naderpour, M; Lund, O Søgaard; Larsen, R

    2008-01-01

    In common bean, Phaseolus vulgaris, four recessive genes, bc-1, bc-2, bc-3 and bc-u control resistance to potyviruses Bean common mosaic virus (BCMV) and Bean common mosaic necrosis virus (BCMNV). To identify candidates for the bc-genes, we cloned and sequenced homologues of genes encoding cap......-binding proteins, eIF4E, eIF(iso)4E and nCBP. In cultivars reported to carry bc-3 resistance, eIF4E was found to display non-silent mutations at codons 53, 65, 76 and 111 closely resembling a pattern of eIF4E mutations determining potyvirus resistance in other plant species. By application of a molecular marker...

  2. Potyviral resistance derived from cultivars of Phaseolus vulgaris carrying bc-3 is associated with homozygotic presence of a mutated eIF4E allele

    DEFF Research Database (Denmark)

    Naderpour, Masoud; Lund, Ole Søgaard; Larsen, Richard

    2010-01-01

    Eukaryotic translation initiation factors (eIFs) play a central role in potyviral infection. Accordingly, mutations in the gene encoding eIF4E have been identified as a source of recessive resistance in several plant species. In common bean, Phaseolus vulgaris, four recessive genes, bc-1, bc-2, bc...

  3. Superhard BC(3) in cubic diamond structure.

    Science.gov (United States)

    Zhang, Miao; Liu, Hanyu; Li, Quan; Gao, Bo; Wang, Yanchao; Li, Hongdong; Chen, Changfeng; Ma, Yanming

    2015-01-01

    We solve the crystal structure of recently synthesized cubic BC(3) using an unbiased swarm structure search, which identifies a highly symmetric BC(3) phase in the cubic diamond structure (d-BC(3)) that contains a distinct B-B bonding network along the body diagonals of a large 64-atom unit cell. Simulated x-ray diffraction and Raman peaks of d-BC(3) are in excellent agreement with experimental data. Calculated stress-strain relations of d-BC(3) demonstrate its intrinsic superhard nature and reveal intriguing sequential bond-breaking modes that produce superior ductility and extended elasticity, which are unique among superhard solids. The present results establish the first boron carbide in the cubic diamond structure with remarkable properties, and these new findings also provide insights for exploring other covalent solids with complex bonding configurations.

  4. Vulgaris

    Directory of Open Access Journals (Sweden)

    Mukaddes Kavala

    2012-01-01

    Full Text Available Background. Thyroid disorders may affect all of the organ systems of the body and they are also highly associated with a wide variety of skin disorders. The aim of this study was to investigate the prevalence of thyroid function abnormalities and thyroid autoimmunity in patients with pemphigus vulgaris (PV and to determine the association between thyroid disorders and clinical involvement and systemic corticosteroid treatment in patients with PV. Methods. The study consisted of eighty patients with PV and eighty healthy individuals. Thyroid functions (fT3, fT4, and TSH and thyroid autoimmunity (anti-thyroid peroxidase (anti-TPO, and anti-thyroglobulin (anti-Tg antibodies were investigated in both groups. Primary thyroid disease (PTD was diagnosed with one or more of the following diagnostic criteria: (i positive antithyroid antibodies, (ii primary thyroid function abnormalities. Results. Significant changes in the serum thyroid profile were found in 16% (13/80 of the PV group and 5% (4/80 of the control group. Positive titers of antithyroid antibodies (anti-TPO and anti-Tg were observed in 7 patients (9% with PV and one in the control group (1,2%. Hashimoto thyroiditis was diagnosed in 9% of PV patients and it was found to be more prevalent in the mucosal form of PV. PTD was found in 13 of (%16 PV patients which was significantly high compared to controls. PTD was not found to be associated with systemic corticosteroid use. Free T3 levels were significantly lower in PV group compared to the control group and free T4 levels were significantly higher in PV group compared to the controls. Conclusions. PV may exist together with autoimmune thyroid diseases especially Hashimoto thyroiditis and primer thyroid diseases. Laboratory work-up for thyroid function tests and thyroid autoantibodies should be performed to determine underlying thyroid diseases in patients with PV.

  5. Architecture for the Secret-Key BC3 Cryptography Algorithm

    Directory of Open Access Journals (Sweden)

    Arif Sasongko

    2011-08-01

    Full Text Available Cryptography is a very important aspect in data security. The focus of research in this field is shifting from merely security aspect to consider as well the implementation aspect. This paper aims to introduce BC3 algorithm with focus on its hardware implementation. It proposes architecture for the hardware implementation for this algorithm. BC3 algorithm is a secret-key cryptography algorithm developed with two considerations: robustness and implementation efficiency. This algorithm has been implemented on software and has good performance compared to AES algorithm. BC3 is improvement of BC2 and AE cryptographic algorithm and it is expected to have the same level of robustness and to gain competitive advantages in the implementation aspect. The development of the architecture gives much attention on (1 resource sharing and (2 having single clock for each round. It exploits regularity of the algorithm. This architecture is then implemented on an FPGA. This implementation is three times smaller area than AES, but about five times faster. Furthermore, this BC3 hardware implementation has better performance compared to BC3 software both in key expansion stage and randomizing stage. For the future, the security of this implementation must be reviewed especially against side channel attack.

  6. Carry

    DEFF Research Database (Denmark)

    Koijen, Ralph S.J.; Moskowitz, Tobias J.; Heje Pedersen, Lasse;

    that include global equities, global bonds, currencies, commodities, US Treasuries, credit, and equity index options. This predictability underlies the strong returns to "carry trades" that go long high-carry and short low-carry securities, applied almost exclusively to currencies, but shown here...

  7. Identification and quantification of the halogenated natural product BC-3

    Energy Technology Data Exchange (ETDEWEB)

    Melcher, J.; Olbrich, D.; Vetter, W. [Hohenheim Univ., Stuttgart (Germany). Inst. fuer Lebensmittelchemie; Marsh, G. [Stockholm Univ. (Sweden). Dept. of Environmental Chemistry; Gaus, C.; Mueller, J.F. [National Research Centre for Environmental Toxicology, Coopers Plains (Australia)

    2004-09-15

    Halogenated natural products (HNPs) of marine origin are increasingly recognized as critical residues in foodstuff (e. g. fish) and environmental samples (e. g. marine mammals and birds). Some of these HNPs (Q1, MHC-1, BC-2, and HDBPs including BC-10) were detected in diverse fish and marine mammal samples at concentrations sometimes exceeding those of PCBs, DDT, and other anthropogenic pollutants. Recent studies with marine mammal samples from Australia led to the detection of six abundant HNPs (Q1, BC-1, BC-2, BC-3, BC-10, and BC-11). In the meantime, Q1 was identified as heptachloro-1{sup '}-methyl-1,2{sup '}-bipyrrole, BC-2 as 4,6-dibromo-2-(2{sup '},4{sup '}-dibromo)phenoxyanisole, BC- 10 as 1,1{sup '}-dimethyl-3,3{sup '},4,4{sup '}-tetrabromo-5,5{sup '}-dichloro-2,2{sup '}-bipyrrole, and BC-11 as 3,5-dibromo- 2-(3{sup '},5{sup '}-dibromo,2{sup '}-methoxy)phenoxyanisole. However the identity of BC-1 and BC-3 remained unclear. The goal of the present study was the identification of BC-3. The tetrabromo compound BC-3 has previously been detected in marine mammals from four continents. Furthermore, we attempted establishing quantitative concentrations in diverse marine biota samples.

  8. On Localized Tachyon Condensation in $\\BC^2/\\BZ_n$ and $\\BC^3/\\BZ_n$

    OpenAIRE

    Sarkar, Tapobrata

    2004-01-01

    We study some aspects of localized tachyon condensation on non-supersymmetric orbifolds of the form $\\BC^2/\\BZ_n$ and $\\BC^3/\\BZ_n$. We discuss the gauged linear sigma models for these orbifolds. We show how several features of the decay of orbifolds of $\\BC^3$ can be realised in terms of orbifolds of $\\BC^2$.

  9. Synthesis of Dense BC3 Phases under High-Pressure and High-Temperature

    Science.gov (United States)

    Zinin, P.; Ming, L.; Acosta, T.; Jia, R.; Hellebrand, E.; Ishii, H.

    2010-12-01

    The finding of the new diamond-like B-C phases is of fundamental importance. These phases are potential high-temperature superconductors and their development is important for understanding the nature of high-temperature superconductivity (Moussa, Cohen, Phys. Rev. B, 77, 064518 2008). They will shed light on the nature of the bonding of the boron atoms in a diamond-like structure. Recently, theoretical simulations of pressure- and temperature-induced phase transition in the B-C system demonstrated that the incorporation of B atoms into a diamond structure should not lead to a drastic distortion of the cubic cell of a diamond (Lowther, J. Phys. Condense Matter. 17, 3221, 2005). In this report we present data on the synthesis of new dense phases cubic BC3 (c-BC3) phase from graphitic BC3 phase (g-BC3) phase under high pressure and high temperature. Two graphitic polycrystalline BCx samples were compressed in a diamond-anvil cell to about 24 GPa and 45 GPa, respectively, and then were laser-heated to ~2000 K. After quenching, each sample was decompressed gradually stepwise to the atmospheric pressure. Synchrotron-based X-ray diffraction patterns were taken before and after the laser-heating, and also at each pressure step-down. The experimental data showed that two new phases were synthesized: (a) an orthorhombic phase with a0 = 3.74 Å, b0 = 3.24 Å, c0=4.25 Å; and (b) a cubic phase with a0 = 3.587Å recovered from 24 GPa and 44 GPa, respectively. The zero-pressure lattice parameter of the cubic phase obtained in this study is larger than that of diamond (i.e., a0=3.5667 Å, ASTM # 6-0675), which is consistent with theoretical prediction The micro-Raman measurements were directly performed on the new phases. The Raman spectra excited by a green (Nd-YAG, 532-nm) laser were taken with a confocal Raman system (WiTec alpha300). The Raman spectrum of the c-BC3 phase is similar to that of diamond-like BC3 phase (Zinin et al., J. Raman Spectrosc., 38, 1362, 2007) with a

  10. Reactivation of Latent Infection of Human Herpesvirus 8 in BC-3 Cells from Primary Effusion Lymphoma by Recombinant CytokinesSimilar to that Produced by HIV-1-infected T Cells

    Institute of Scientific and Technical Information of China (English)

    卢春; 曾怡; 黄丽

    2003-01-01

    Objective:To study and confirm that recombinant cytokines similar to those produced by HIV-1 infected T cells induced lytic cycle replication of human pervirus 8(HHV-8) in BC-3 cells,another cell line from primary effusion lymphoma(PEL).Methods:The persistent stimulation of BC-3 was conducted by several cytokines known to be produced by HIV-1-infected T cells and important in grouth and proliferation of Kaposi's sarcoma(KS) cells in vitro,such as the inter feron-γ(IFN-γ),the hepatocyte grouth factor /scatter factor (HGF/SF),the Oncostain M(OSM),and the tumor necrosis factor-α(TNF-α)which is not produced by HIV-1-infeted T cells.Treated and antrented BC-3 cells were collected at the 3rd and 7th day of persistent stimulation,respeclively.Immunohistochemical(IHC) staining. Northern blot,quantitative PCR(real-time PCR) and electron microscopy(EM) were carried out to detect the expression of immumogenic protein ORF59,messenger RNA(mRNA) of minor capsid protein ORF26,and the presence of viral particles of HHV-8 from treated and untreated BC-3 cells.Results:It showed that IFN-γ,HGF/SF/OSM,and TNF-α were found to induce an increase in mRNA expression of ORF26 when added individually to BC-3 cells.Particularly,ORF26 expression stimulated with IFN-γ and TNF-α respectively,increased 6.1 and 2.5-fold(from,real-time PCR results) at the 7th day when compared with untreated BC-3 cells.Meanwhile ,about 20% of IFN-γ stimulated BC-3 cells expressed ORF59 at the 7th day as compared with 1.5% of untreated BC-3 cells when IHC staining was employed.In addition,viral particles of HHV-8 were readily idelified in BC-3 cells stimulated with IFN-γ at the 7th day with EM analysis.Conclusion:TNF-α and recombinant cytokines being similar to those produced by HIV-1 infected T Cells could really induce HHV-8 lytic cycle replication in BC-3 cells,another cell line of PEL.

  11. Mitotic Catastrophe in BC3H1 Cells following Yessotoxin Exposure

    Science.gov (United States)

    Korsnes, Mónica Suárez; Korsnes, Reinert

    2017-01-01

    The marine toxin yessotoxin (YTX) can cause various cytotoxic effects depending on cell type and cell line. It is well known to trigger distinct mechanisms for programmed cell death which may overlap or cross-talk. The present contribution provides the first evidence that YTX can cause genotoxicity and induce mitotic catastrophe which can lead to different types of cell death. This work also demonstrates potential information gain from non-intrusive computer-based tracking of many individual cells during long time. Treatment of BC3H1 cells at their exponential growth phase causes atypical nuclear alterations and formation of giant cells with multiple nuclei. These are the most prominent morphological features of mitotic catastrophe. Giant cells undergo slow cell death in a necrosis-like manner. However, apoptotic-like cell death is also observed in these cells. Electron microscopy of treated BC3H1 cells reveal uncondensed chromatin and cells with double nuclei. Activation of p-p53, p-H2AX, p-Chk1, p-ATM, and p-ATR and down-regulation of p-Chk2 indicate DNA damage response and cell cycle deregulation. Micronuclei formation further support this evidence. Data from tracking single cells reveal that YTX treatment suppresses a second round of cell division in BC3H1 cells. These findings suggest that YTX can induce genomic alterations or imperfections in chromosomal segregation leading to permanent mitotic failure. This understanding extends the list of effects from YTX and which are of interest to control cancer and tumor progression.

  12. DFT studies of Si- and Al-doping effects on the acetone sensing properties of BC3 graphene

    Science.gov (United States)

    Moradi, Morteza; Noei, Maziar; Ahmadi Peyghan, Ali

    2013-11-01

    In order to find a novel sensor, reactivity and sensitivity of the intrinsic, Al- and Si-doped BC3 graphene-like sheets to an acetone molecule were investigated by using B3LYP and ωB97X-D density functional calculations. Adsorption of acetone on the intrinsic, Al- and Si-doped BC3 sheets releases energies of about 7.2, 36.5 and 24.8 kcal/mol, respectively, using ωB97X-D. The Si-doped sheet presents high sensitivity to acetone compared with the intrinsic and Al-doped sheets indicated by the calculated geometrical structures and electronic properties for these systems. The HOMO/LUMO energy gap of Si-doped BC3 sheet is significantly decreased from 2.20 to 1.65 eV (B3LYP), which would result in electrical conductance increment. Thus, Si-doped sheet are expected to be a potential candidate for detecting the presence of acetone.

  13. Ichthyosis vulgaris

    DEFF Research Database (Denmark)

    Thyssen, J P; Godoy-Gijon, E; Elias, P M

    2013-01-01

    Ichthyosis vulgaris is caused by loss-of-function mutations in the filaggrin gene (FLG) and is characterized clinically by xerosis, scaling, keratosis pilaris, palmar and plantar hyperlinearity, and a strong association with atopic disorders. According to the published studies presented...... in this review article, FLG mutations are observed in approximately 7·7% of Europeans and 3·0% of Asians, but appear to be infrequent in darker-skinned populations. This clinical review article provides an overview of ichthyosis vulgaris epidemiology, related disorders and pathomechanisms. Not only does...... ichthyosis vulgaris possess a wide clinical spectrum, recent studies suggest that carriers of FLG mutations may have a generally altered risk of developing common diseases, even beyond atopic disorders. Mechanistic studies have shown increased penetration of allergens and chemicals in filaggrin...

  14. Pemphigus vulgaris

    Directory of Open Access Journals (Sweden)

    Sandhya Tamgadge

    2011-01-01

    Full Text Available Pemphigus vulgaris is a chronic autoimmune mucocutaneous disease that initially manifests in the form of intraoral lesions, which spread to other mucous membranes and the skin. The etiology of pemphigus vulgaris is still unknown, although the disease has attracted considerable interest. The pemphigus group of disease is characterized by the production of autoantibodies against intercellular substances and is thus classified as autoimmune diseases. Most patients are initially misdiagnosed and improperly treated for months or even years. Dental professionals must be sufficiently familiar with the clinical manifestations of pemphigus vulgaris to ensure early diagnosis and treatment, since this in turn determines the prognosis and course of the disease. This article presents a case report with unknown etiology along with an overview of the disease.

  15. Acne vulgaris

    OpenAIRE

    Purdy, Sarah; DeBerker, David

    2008-01-01

    Acne vulgaris affects over 80% of teenagers, and persists beyond the age of 25 years in 3% of men and 12% of women. Typical lesions of acne include comedones, inflammatory papules, and pustules. Nodules and cysts occur in more severe acne, and can cause scarring and psychological distress.

  16. Acne vulgaris

    OpenAIRE

    Purdy, Sarah; de Berker, David

    2011-01-01

    Acne vulgaris affects over 80% of teenagers, and persists beyond the age of 25 years in 3% of men and 12% of women. Typical lesions of acne include comedones, inflammatory papules, and pustules. Nodules and cysts occur in more severe acne, and can cause scarring and psychological distress.

  17. BC3EE2,9B, a synthetic carbazole derivative, upregulates autophagy and synergistically sensitizes human GBM8901 glioblastoma cells to temozolomide.

    Science.gov (United States)

    Chen, Chien-Min; Syu, Jhih-Pu; Way, Tzong-Der; Huang, Li-Jiau; Kuo, Sheng-Chu; Lin, Chung-Tien; Lin, Chih-Li

    2015-11-01

    Glioblastoma multiforme (GBM) is the most fatal form of human brain cancer. Although temozolomide (TMZ), an oral alkylating chemotherapeutic agent, improves the survival rate, the prognosis of patients with GBM remains poor. Naturally occurring carbazole alkaloids isolated from curry leaves (Murraya koenigii Spreng.) have been shown to possess a wide range of anticancer properties. However, the effects of carbazole derivatives on glioblastoma cells remain poorly understood. In the present study, anti‑glioblastoma profiles of a series of synthetic carbazole derivatives were evaluated in vitro. The most promising derivative in this series was BC3EE2,9B, which showed significant anti‑proliferative effects in GBM8401 and GBM8901 cells. BC3EE2,9B also triggered cell‑cycle arrest, most prominently at the G1 stage, and suppressed glioblastoma cell invasion and migration. Furthermore, BC3EE2,9B induced autophagy‑mediated cell death and synergistically sensitized GBM cells to TMZ cytotoxicity. The possible mechanism underlying BC3EE2,9B‑induced autophagy may involve activation of adenosine monophosphate-activated protein kinase and the attenuation of the Akt and mammalian target of the rapamycin downstream signaling pathway. Taken together, the present results provide molecular evidence for the mode of action governing the ability of BC3EE2,9B to sensitize drug‑resistant glioblastoma cells to the chemotherapeutic agent TMZ.

  18. Acne vulgaris.

    Science.gov (United States)

    Moradi Tuchayi, Sara; Makrantonaki, Evgenia; Ganceviciene, Ruta; Dessinioti, Clio; Feldman, Steven R; Zouboulis, Christos C

    2015-09-17

    Acne vulgaris is a chronic inflammatory disease - rather than a natural part of the life cycle as colloquially viewed - of the pilosebaceous unit (comprising the hair follicle, hair shaft and sebaceous gland) and is among the most common dermatological conditions worldwide. Some of the key mechanisms involved in the development of acne include disturbed sebaceous gland activity associated with hyperseborrhoea (that is, increased sebum production) and alterations in sebum fatty acid composition, dysregulation of the hormone microenvironment, interaction with neuropeptides, follicular hyperkeratinization, induction of inflammation and dysfunction of the innate and adaptive immunity. Grading of acne involves lesion counting and photographic methods. However, there is a lack of consensus on the exact grading criteria, which hampers the conduction and comparison of randomized controlled clinical trials evaluating treatments. Prevention of acne relies on the successful management of modifiable risk factors, such as underlying systemic diseases and lifestyle factors. Several treatments are available, but guidelines suffer from a lack of data to make evidence-based recommendations. In addition, the complex combination treatment regimens required to target different aspects of acne pathophysiology lead to poor adherence, which undermines treatment success. Acne commonly causes scarring and reduces the quality of life of patients. New treatment options with a shift towards targeting the early processes involved in acne development instead of suppressing the effects of end products will enhance our ability to improve the outcomes for patients with acne.

  19. 农杆菌介导灰葡萄孢菌株RoseBc-3的遗传转化%Agrobacterium tumefaciens-mediated transformation of Botrytis cinerea strain RoseBc-3

    Institute of Scientific and Technical Information of China (English)

    范雷; 张静; 杨龙; 李国庆

    2013-01-01

    In this paper, we reported results about characterization of the factors affecting Agrobac-terium tumefaciens-mediated transformation (ATMT) of the plant pathogenic fungus Botrytis cinerea strain RoseBc-3 and about the validity of ATMT for the particular strain of B. cinerea. The results showed that the temperatures for co-incubation of A. tumefaciens and B. cinerea and the conidial concentration of B. cinerea in the co-incubation mixture greatly affected the transformation efficiency. The optimal co-incubation temperature was 22 ℃ and the optimal conidial concentration was 1 × 105 conidia/ mL. Under the optimal co-incubation conditions, the numbers of transformant reached 100 per dish (9 cm diameter). All the 16 randomly-selected transformants of B. cinerea were confirmed to harbor the T-DNA of A. tumefaciens based on the result of the specific PCR. The result of the Southern blotting further indicated the integration of the T-DNA in the genome of B. cinerea with the frequency of the single-copy insertion reaching 87. 5%. Six types of mutants were identified from the transformants. They were sporulation deficiency mutants (3. 8%), mycelial growth-reduced mutants (3. 5%), sparse mycelia mutants (1. 6%), colony color mutants (5. 7%), pathogenicity decreasing mutants (3. 3%) and patho-genicity enhancing mutants (3. 0%). DNA sequences flanking the T-DNA tag were obtained using the inverse PCR technique and the hi-TAIL PCR technique from 14 mutants of B. cinerea. The insertion sites in these mutants were located with the same frequency in the coding region and in the non-coding regions.%观察了农杆菌(Agrobacterium tumefaciens)介导转化灰葡萄孢(Botrytis cinerea)菌株RoseBc-3的影响因素,并对遗传转化的有效性进行评估.结果表明:农杆菌与灰葡萄孢共培养的温度和灰葡萄孢分生孢子浓度均能影响转化效率,当共培养温度为22℃、灰葡萄孢分生孢子浓度为1×105个/mL时,转化效

  20. Carrying Capacity

    DEFF Research Database (Denmark)

    Schroll, Henning; Andersen, Jan; Kjærgård, Bente

    2012-01-01

    A spatial planning act was introduced inIndonesia 1992 and renewed in 2008. It emphasised the planning role of decentralised authorities. The spatial planning act covers both spatial and environmental issues. It defines the concept of carrying capacity and includes definitions of supportive...... carrying capacity (SCC) and assimilative carrying capacity (ACC). The act mandates that the latter two aspects must be taken into consideration in the local spatial plans. The present study aimed at developing a background for a national guideline for carrying capacity in Indonesian provinces and districts...... standard or governmental political objective exists. In most cases it was possible to select a set of indicators, including thresholds that are workable in a carrying capacity planning at the local administrative levels. Not all relevant sectors at the decentralized level were included. Indicators of SCC...

  1. Carrying Capacity

    DEFF Research Database (Denmark)

    Schroll, Henning; Andersen, Jan; Kjærgård, Bente

    2012-01-01

    A spatial planning act was introduced inIndonesia 1992 and renewed in 2008. It emphasised the planning role of decentralised authorities. The spatial planning act covers both spatial and environmental issues. It defines the concept of carrying capacity and includes definitions of supportive...... and ACC may increase the political focus on resources and environmental issues and may help to move local authorities towards a more holistic spatial planning approach. A carrying capacity approach could be an inspiration for local spatial planning in developing countries. A spatial planning act...... was introduced inIndonesia 1992 and renewed in 2008. It emphasised the planning role of decentralised authorities. The spatial planning act covers both spatial and environmental issues. It defines the concept of carrying capacity and includes definitions of supportive carrying capacity (SCC) and assimilative...

  2. Light attenuation on Chlorella vulgaris cells

    Science.gov (United States)

    Krol, Tadeusz; Lotocka, Maria

    1993-12-01

    The laboratory measurements of spectrum of light attenuation on phytoplankton particles i.e. monoculture of unicellural green algae Chlorella vulgaris are presented. The measurements were carried out for alive culture and the cultures subjected to chemical (NaOH) or physical (ultrasounds) modification. The distinct changes in the light attenuation spectrum were a result of modification of the internal cell structures.

  3. OXIDANT/ANTIOXIDANT STATUS IN OBESE ADOLESCENT FEMALES WITH ACNE VULGARIS

    OpenAIRE

    Abulnaja Khalid

    2009-01-01

    Background and Objectives: Acne vulgaris is a distressing skin condition, which can carry with it significant psychological disability. Oxidant/antioxidant imbalance leads to increased production of free radicals, that cause many diseases. Some nutrients, along with systemic oxidative stress, have been implicated in acne vulgaris. The goal of the present study was to assess oxidant and antioxidant status in correlation with the incidence of acne vulgaris in adolescent obese females. Materia...

  4. Lupus vulgaris of external nose.

    Science.gov (United States)

    Bhandary, Satheesh Kumar; Ranganna, B Usha

    2008-12-01

    Lupus vulgaris is the commonest form of cutaneous tuberculosis which commonly involve trunk and buttocks. Lupus vulgaris affecting nose and face, are rarely reported in India. This study reports an unusual case of lupus vulgaris involving the external nose that showed dramatic outcome after six months of anti- tubercular treatment.

  5. Lupus vulgaris: difficulties in diagnosis.

    Science.gov (United States)

    Rhodes, Julia; Caccetta, Tony Philip; Tait, Clare

    2013-05-01

    Lupus vulgaris is one of the most common forms of cutaneous tuberculosis. It presents a diagnostic challenge due to its paucibacillary nature. This is a report of a case of a delayed diagnosis of lupus vulgaris, presenting as perianal and peristomal plaques, followed by a review of the diagnostic tools for lupus vulgaris and their limitations.

  6. Psoriasiform lupus vulgaris.

    Science.gov (United States)

    Padmavathy, L; Rao, L Lakshmana; Ethirajan, N; Dhanlaklshmi, M

    2008-04-01

    Tuberculosis is a major public health problem in both developing and developed countries. Cutaneous Tuberculosis constitutes a minor proportion of extra-pulmonary manifestations of Tuberculosis. Lupus Vulgaris (LV) is one of the clinical variants of Cutaneous Tuberculosis. A case of a large plaque type psoriasiform lesion of lupus vulgaris on the thigh, of 15 years' duration, in an 18-year-old girl is reported. This case highlights the ignorance level among the patients and consequent failure to avail proper anti-tuberculous treatment despite campaign in print and audio visual media.

  7. Strongylus vulgaris and colic

    DEFF Research Database (Denmark)

    Nielsen, Martin Krarup; Jacobsen, Stine; Olsen, Susanne Nautrup

    Strongylus vulgaris is regarded the most pathogenic helminth parasite infecting horses. It was once estimated to be the primary cause of colic in horses and has been termed the horse killer. Disease is ascribed to thromboembolism caused by larvae migrating in the mesenteric arteries eventually le...

  8. Lung and lupus vulgaris.

    Science.gov (United States)

    Mukta, V; Jayachandran, K

    2011-04-01

    Lupus vulgaris is chronic, postprimary, paucibacillary cutaneous tuberculosis found in individuals with moderate immunity and high degree of tuberculin sensitivity. Eighty percent of the lesions are on the head and neck. We present the case of a 38 year old lady who was admitted with complaints of worsening breathlessness and low grade fever of one month duration. Examination showed multiple, nontender skin ulcers on bilateral lumbar areas, two oozing serosanguinous discharge and others scarred in the centre. Respiratory system examination and chest X-ray revealed right sided pleural effusion. On investigation, pleural fluid was tuberculous in nature. Skin biopsy from the edge of ulcer was also suggestive of tuberculosis. Patient is doing well on antituberculous drugs. This case highlights the importance of cutaneous manifestations of systemic disease and is an example of the unusual presentation of lupus vulgaris in a case of pleural effusion.

  9. Pemphigus vulgaris in pregnancy.

    Science.gov (United States)

    Salzberg, Kelsey W; Gero, Melanie J; Ragsdale, Bruce D

    2014-10-01

    We report the case of a 34-year-old woman who was diagnosed with pemphigus vulgaris (PV) during pregnancy. The patient presented with widespread blistering dermatitis and associated burning and pruritus. At 6 weeks' gestation the patient was admitted to the hospital to expedite her diagnosis and initiate treatment. A skin biopsy revealed suprabasal acantholysis, and direct immunofluorescence demonstrated diffuse intercellular IgG in the epidermis and basal intercellular C3, which confirmed the diagnosis of PV. Treatment with corticosteroids was instituted after discussions with the patient about possible adverse effects to the fetus. Pemphigus vulgaris is rare in pregnancy and active PV presents potential threats of fetal spread and transient lesion production, which is associated with increased mortality and morbidity in the fetus. Our patient had active PV and required treatment throughout her pregnancy. The pregnancy progressed to premature delivery of the neonate without skin lesions or apparent complications.

  10. Depsides from Prunella vulgaris

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    A novel depsides 1, together with three known ones 2~4 and two phenylpropanoids 5~6 were isolated from the ethanol extract of the spikes of Prunella vulgaris. On the basis of spectral and chemical evidence, their structures were deternined as butyl rosmarinate 1, ethyl rosmarinate 2, methyl rosmarinate 3, rosmarinic acid 4, 3,4,a -trihydroxy-methyl phenylpropionate 5 and p-coumaric acid 6, respectively.

  11. Thymus vulgaris subsp. mansanetianus subsp. nov. (Lamiaceae)

    OpenAIRE

    Pedro Pablo Ferrer-Gallego; Albert J. Navarro Peris; Emilio Laguna Lumbreras; Gonzalo Mateo Sanz

    2013-01-01

    RESUMEN: Se describe una nueva subespecie de Thymus vulgaris L. (Lamiaceae); Th. vulgaris subsp. mansanetianus subsp. nov., caracterizada por presentar un hábito postrado, tallos estoloníferos, decumbentes y radicantes, hojas muy estrechas y una floración otoñal. ABSTRACT: Thymus vulgaris subsp. mansanetianus subsp. nov. (Lamiaceae). A new subspecies of Thymus vulgaris L. (Lamiaceae); Th. vulgaris subsp. mansanetianus subsp. nov. is described. This new subspecies is characterized by its prost...

  12. Detection of filaggrin gene mutation (2282del4) in Pakistani Ichthyosis vulgaris families.

    Science.gov (United States)

    Naz, Naghma; Samdani, Azam Jah

    2011-06-01

    The aim of this study was to detect an 811 bp filaggrin (FLG) gene fragment known to carry a mutation 2282del4 which causes ichthyosis vulgaris. Seven clinically examined ichthyosis vulgaris families were included in this study. An 811 bp FLG gene fragment was targeted in the genomic DNA of all the members of the seven families by PCR amplification using known primers RPT1P7 and RPT2P1. Successful amplification of an 811 bp FLG gene fragment in all the families suggested the possible role of the 2282del4 mutation in causing ichthyosis vulgaris in Pakistani population.

  13. The immunogenetics of pemphigus vulgaris.

    Science.gov (United States)

    Gazit, Ephraim; Loewenthal, Ron

    2005-01-01

    Pemphigus vulgaris (PV) is an autoimmune blistering disease of the skin of unknown etiology. While various environmental factors have been implicated as triggering agents, HLA association is probably the most important predisposing factor. The aim of this review is to highlight the association of HLA with pemphigus vulgaris. In addition, we present recent results showing a possible association with the nonclassical HLA-G antigen.

  14. Nail manifestations in pemphigus vulgaris

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    Cahali Juliana Burihan

    2002-01-01

    Full Text Available Nail involvement in pemphigus vulgaris is rare. We describe 5 patients with pemphigus vulgaris presenting nail involvement. In this disease, nail manifestations present, by order of frequency, as chronic paronychia, onychomadesis, onycholysis, Beau's lines and trachyonychia. All our 5 cases presented with paronychia, and 1 of them also had Beau's lines. Treatment with prednisone and/or cyclophosphamide controlled mucocutaneous and nail manifestations in all cases.

  15. [Acne vulgaris: endocrine aspects].

    Science.gov (United States)

    Dekkers, O M; Thio, B H; Romijn, J A; Smit, J W A

    2006-06-10

    Androgens play an important part in the development of acne vulgaris. Androgen levels in patients with acne are higher than those in controls and people with the androgen insensitivity syndrome do not develop acne. Local factors other than androgen plasma levels, also play a part in the development of acne. The skin contains enzymes that convert precursor hormones to the more potent androgens such as testosterone and dihydrotestosterone. Androgen synthesis can therefore be regulated locally. The effects of androgens on the skin are the result of circulating androgens and enzyme activity in local tissues and androgen receptors. Acne is a clinical manifestation of some endocrine diseases. The polycystic ovary syndrome has the highest prevalence. In women with acne that persists after puberty, in 10-200% of cases polycystic ovary syndrome is later diagnosed. The mechanism of hormonal anti-acne therapy may work by blocking the androgen-production (oestrogens) or by blocking the androgen receptor (cyproterone, spironolactone).

  16. Pemphigus Vulgaris Presented with Cheilitis

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    Zaheer Abbas

    2014-01-01

    Full Text Available Background. Pemphigus vulgaris is an autoimmune blistering disease affecting the mucous membrane and skin. In 50 to 70% of cases, the initial manifestations of pemphigus vulgaris are oral lesions which may be followed by skin lesions. But it is unusual for the disease to present with initial and solitary persistent lower lip lesions without progression to any other location. Main Observations. We report a 41-year-old woman with dry crusted lesions only on the lower lip, clinically resembling actinic cheilitis and erosive lichen planus, but histopathological evaluation showed unexpected results of suprabasal acantholysis and cleft compatible with pemphigus vulgaris. We treated her with intralesional triamcinolone 10 mg/mL for 2 sessions and 2 g cellcept daily. Patient showed excellent response and lesions resolved completely within 2 months. In one-year follow-up, there was no evidence of relapse or any additional lesion on the other sites. Conclusion. Cheilitis may be the initial and sole manifestation of pemphigus vulgaris. Localized and solitary lesions of pemphigus vulgaris can be treated and controlled without systemic corticosteroids.

  17. Signalling pathways in pemphigus vulgaris.

    Science.gov (United States)

    Li, Xiaoguang; Ishii, Norito; Ohata, Chika; Furumura, Minao; Hashimoto, Takashi

    2014-03-01

    Acantholysis in pemphigus vulgaris is induced by binding of autoantibodies to desmoglein 3 (Dsg3). The roles of signalling pathways on development of acantholysis have recently been extensively studied. In the study by Sayar et al., recently published in Exp Dermatol, epidermal growth factor receptor (EGFR) signalling was activated in both in vivo and in vitro pemphigus vulgaris experimental models. However, while EGFR inhibitors suppressed activity of p38 mitogen-activated protein kinase (p38MAPK) linearly, they suppressed activity of c-Myc and acantholysis in a non-linear, V-shaped relationship. These findings indicated complicated interactions among EGFR, p38MAPK and c-Myc in pemphigus vulgaris pathology.

  18. Atopic dermatitis and ichthyosis vulgaris.

    Science.gov (United States)

    Rabinowitz, L G; Esterly, N B

    1994-06-01

    Atopic dermatitis remains a common skin problem in the pediatric age group. General approaches to management focus on reducing inflammation and pruritus as well as preventing xerosis. Ichthyosis vulgaris is the most common form of the ichthyoses and often is associated with atopic dermatitis. Recognition of these conditions is necessary to institute therapy that will alleviate the discomfort experienced by affected individuals.

  19. Porokeratosis Masquerading As Lupus Vulgaris

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    Das Sudip

    2004-01-01

    Full Text Available Porokeratosis is a specific disorder of kerstinization characterized histologically by cornoid lamella. Lesions of porokeratosis present varied features, often mimicking other dermatological disorders. Herein, we report a case of porokeratosis of Mibelli presenting with verrucous lesions simulating lupus vulgaris.

  20. Disseminated lupus vulgaris.

    Science.gov (United States)

    Garg, Taru; Ramchander; Shrihar, Rashmi; Gupta, Tanvi Pal; Aggarwal, Shilpi

    2011-01-01

    follicular plugging and multiple epithelioid cell granulomas, rimmed by lymphocytes in the deeper portion of the dermis, mainly peri-appendageal. Stain for acid-fast bacteria was negative. Cultures from the skin lesions were negative. The patient was diagnosed as having lupus vulgaris with multiple lesions of varying morphology at different sites with pulmonary tuberculosis and healed lymph node involvement.

  1. Standardization of phaseolus vulgaris valves by macro- and microscopic characteristics

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    Анна Ігорівна Крюкова

    2016-03-01

    Full Text Available During the last years on the Ukrainian pharmaceutical market a tendency to increase the range of the national herbal remedies is observed. Native flora herbs, having a sufficient resource base, deserve special attention and are characterized by a number of economic benefits for manufacturers. The implementation of herbal remedies in medical practice is accompanied by the necessity to improve the quality control of herbal material and to develop normative documents. Macro- and microscopy analysis becomes important at standardization of herbal material especially cut and powdered.Aim. The aim of our work was to carry out macro- and microscopy research of Phaseolus vulgaris and to determine its diagnostic morphologic and anatomic features.Methods. The conventional research methods in pharmacognosy have been used; photomicrography has been taken by using microscope MC-10 and Samsung PL 50 camera.Results. As a result of research, the microscopic diagnostic characteristics for Phaseolus vulgaris valves have been proposed, e.g.: the valves are elongated, straight, navicular or grooved, twisted to some extent; the external surface is smooth or slightly undulating, mat, pale yellow or yellow; the internal surface is shiny, white or yellowish-white. Sometimes carpophore (stalk may occur. The microscopic diagnostic features have been determined and offered, e.g.: cells of outer epidermis are pentagonal and hexagonal, with plicated cuticle; stomata are surrounded by 3–4 subsidiary narrow cells, additionally surrounded by 5–6 narrow cells; cover trichomes and glandular trichomes are elongated, pointed and unbranched; xylem vessels and tracheids; non-bundled structure stalk fragments with pericyclic sclerenchyma and secretory cells in the phloem.Conclusion. Research on determination of the macro- and microscopic characteristics of Phaseolus vulgaris valves has been carried out. The diagnostic morphological and anatomic elements in herbal material have

  2. Pemphigus vulgaris in adolescence: a case report

    OpenAIRE

    Ferreira, Jean Paulo Rodolfo [UNESP; Araújo, Paula Caetano [UNESP; SAliba, Marcos Tadeu Adas [UNESP; Consolaro,Renata Bianco; Garbin,Cléa Adas Saliba

    2013-01-01

    Pemphigus vulgaris is an autoimmune disease, normally associated with oral lesions that generally affects patients aged 40 - 60 years. The aim of this study was to report a case of pemphigus vulgaris presenting at an uncommon age with advanced periodontal disease, and to describe the treatment performed from the time of diagnosis through oral environment restoration and follow-up monitoring. One gingival vesicular lesion was biopsied. After the histological confirmation of pemphigus vulgaris,...

  3. Extensive keloid formation after pemphigus vulgaris

    OpenAIRE

    Sako, Eric Y; Worswick, Scott

    2015-01-01

    Pemphigus vulgaris is an immunobullous disease characterized by intraepidermal blister formation. These blisters eventually rupture, leaving erosions that are slow to heal, often leaving hyperpigmented patches, but no scars. We describe a case of a 67- year-old man with pemphigus vulgaris who suffered severe keloidal scarring after the pemphigus lesions became infected. His keloids were treated with intralesional corticosteroids with some improvement. Pemphigus vulgaris, a process confined to...

  4. Extensive keloid formation after pemphigus vulgaris.

    Science.gov (United States)

    Sako, Eric Y; Workwick, Scott

    2015-11-18

    Pemphigus vulgaris is an immunobullous disease characterized by intraepidermal blister formation. These blisters eventually rupture, leaving erosions that are slow to heal, often leaving hyperpigmented patches, but no scars. We describe a case of a 67- year-old man with pemphigus vulgaris who suffered severe keloidal scarring after the pemphigus lesions became infected. His keloids were treated with intralesional corticosteroids with some improvement. Pemphigus vulgaris, a process confined to the epidermis, may lead to scarring in predisposed individuals, particularly if infection occurs.

  5. Analysis of Sister Carrie

    Institute of Scientific and Technical Information of China (English)

    孙淑珍

    2004-01-01

    Chapter Ⅰ Introduction  Sitting in the rocking chair,Carrie dreams her future.This is the deep impression the novel"Sister Carrie"gives us,which is written by Theodore Dreiser(1871-1945),the great American realism writer.  ……

  6. Therapeutic Agents in Acne Vulgaris

    Science.gov (United States)

    Stewart, Wm. D.; Maddin, Stuart; Nelson, A. J.; Danto, J. L.

    1963-01-01

    A total of 120 consecutive patients with pustular and cystic acne vulgaris were selected for study. Patients were assigned a placebo and a tetracycline medication in a random method. Of the 53 patients who were given tetracycline, 45 showed some response, which was fair in 19 and excellent in 26. Of the 55 patients who received placebo, 24 showed no response while 31 showed some improvement. No side effects were reported. The difference in response between the two groups is statistically significant. It is concluded that administration of 250 mg. tetracycline four times daily, even for periods as short as two weeks, enhances the likelihood of improvement of cystic or pustular acne vulgaris. PMID:14079132

  7. Aspek Imunologis pada Pemphigus Vulgaris

    Directory of Open Access Journals (Sweden)

    Saraventi Saraventi

    2015-10-01

    Full Text Available Pemphigus vulgaris is a relatively uncommon autoimmune disease marked with blister appearance of the skin tissue and mucosal membrane, resulting in loss of intercellular adhesion as well as the intact of epithelial cells, or so-called acantholysis. In this autoimmune disease the immune system forms autoantibodies attacking desmoglein, a specific protein at the skin tissue. The predisposing factors are presumed to include genetic and ethnic origin of the patient. Although at first usually without symptoms, after proceeding to the ulcerating stage the disease can result in fatal complications. Considering that almost 60% of the cases are first manifested in the oral mucosa, it is important that the dentists are aware of the basic etiology and immunology of the disease for correct diagnosis and therapy. This work reviews the etiopathogenesis of Pemphigus vulgaris, with emphasis on immunological aspects and immunotherapy.

  8. GLYCOSIDES FROM LINARIA VULGARIS MILL

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    Natalia Mashcenko

    2008-12-01

    Full Text Available A new flavonol glycoside, 5,4′-dimethylkaempferol 3-O-β-D-(6′′-α-Lrhamnopyranosyl -glucopyranoside, together with three known compounds were isolated from the n-butanolic soluble fraction of underground and aerial parts of Linaria vulgaris Mill, collected on the territory of Moldova. The characterisation of these compounds was achieved by various chromatographic and spectroscopic methods (IR, UV, 13C-NMR, 1H-NMR and MS.

  9. Unrecognized dermatophyte infection in ichthyosis vulgaris.

    Science.gov (United States)

    Grahovac, Maja; Budimcić, Dragomir

    2009-01-01

    A case of unrecognized widespread dermatophyte infection associated with ichthyosis vulgaris and atopy is described. Our patient was a young woman in which the diagnosis of ichthyosis vulgaris and atopic dermatitis blocked the recognition of widespread dermatophyte infection for more than six months. The case showed some clinical peculiarities in terms of both extent of lesions and their clinical appearance.

  10. Sporotrichoid lupus vulgaris: A rare presentation.

    Science.gov (United States)

    Maheshwari, Anshul; Tiwari, Siddhi; Mathur, Deepak K; Bhargava, Puneet

    2015-01-01

    Lupus vulgaris is the most common presentation of cutaneous tuberculosis in India and can present as papular, nodular, plaque, ulcerative, vegetating, and tumid forms. Unusual variants include the frambesiform, gangrenous, ulcerovegetating, lichen simplex chronicus, myxomatous, and sporotrichoid types. We describe a rare sporotrichoid presentation of lupus vulgaris on the leg of a 28-year-old female of 12 years duration.

  11. Pemphigus vulgaris with solitary toxic thyroid nodule.

    Science.gov (United States)

    Alfishawy, Mostafa; Anwar, Karim; Elbendary, Amira; Daoud, Ahmed

    2014-01-01

    Background. Pemphigus vulgaris is an autoimmune vesiculobullous disease, affecting the skin and mucous membranes. It is reported to be associated with other autoimmune diseases including autoimmune thyroid diseases. However we report herein a case of pemphigus vulgaris associated with autonomous toxic nodule. Case Presentation. A 51-year-old woman was evaluated for blisters and erosions that develop on her trunk, face, and extremities, with a five-year history of progressively enlarging neck mass, and a past medical history of pemphigus vulgaris seven years ago. The condition was associated with palpitation, dyspnea, and heat intolerance. Thyroid function tests and thyroid scan were compatible with the diagnosis of thyrotoxicosis due to autonomous toxic nodule. Exacerbation of pemphigus vulgaris was proved by skin biopsy from the patient which revealed histologic picture of pemphigus vulgaris. Conclusion. Autoimmune thyroid diseases are reported to associate pemphigus vulgaris. To our knowledge, this case is the first in the English literature to report association between pemphigus vulgaris and autonomous toxic nodule and highlights the possibility of occurrence of pemphigus vulgaris with a nonautoimmune thyroid disease raising the question: is it just a coincidence or is there an explanation for the occurrence of both conditions together?

  12. Pemphigus Vulgaris with Solitary Toxic Thyroid Nodule

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    Mostafa Alfishawy

    2014-01-01

    Full Text Available Background. Pemphigus vulgaris is an autoimmune vesiculobullous disease, affecting the skin and mucous membranes. It is reported to be associated with other autoimmune diseases including autoimmune thyroid diseases. However we report herein a case of pemphigus vulgaris associated with autonomous toxic nodule. Case Presentation. A 51-year-old woman was evaluated for blisters and erosions that develop on her trunk, face, and extremities, with a five-year history of progressively enlarging neck mass, and a past medical history of pemphigus vulgaris seven years ago. The condition was associated with palpitation, dyspnea, and heat intolerance. Thyroid function tests and thyroid scan were compatible with the diagnosis of thyrotoxicosis due to autonomous toxic nodule. Exacerbation of pemphigus vulgaris was proved by skin biopsy from the patient which revealed histologic picture of pemphigus vulgaris. Conclusion. Autoimmune thyroid diseases are reported to associate pemphigus vulgaris. To our knowledge, this case is the first in the English literature to report association between pemphigus vulgaris and autonomous toxic nodule and highlights the possibility of occurrence of pemphigus vulgaris with a nonautoimmune thyroid disease raising the question: is it just a coincidence or is there an explanation for the occurrence of both conditions together?

  13. Distribution and Development of the TCM Syndromes in Psoriasis Vulgaris

    Institute of Scientific and Technical Information of China (English)

    ZHANG Guang-zhong; WANG Ju-sheng; WANG Ping; JIANG Chun-yan; DENG Bing-xu; LI Ping; ZHAO Yi-ming; LIU Wa-li; Qu Xing; CHEN Wei-wen; ZENG Lin; ZHOU Dong-mei; SUN Li-yun; LI Ruo-yu

    2009-01-01

    Objective:To study the distribution and development rules of the TCM syndromes in psoriasis vulgaris.Methods:Based on the clinical epidemiologic mass survey, the study was carried out by means of a status survey, multi-center and large-sample research.The data base was set up by EPIINFO6.0.The SPSS was used to do the statistical analyses in 2651 cases of psoriasis vulgaris to study the correlations among the distribution and development of the TCM syndromes, the stages of the disease, nationality, psoriasis history,family history, smoking history, alcohol drinking history, and severity of the disease.Results:The TCM syndromes in psoriasis vulgaris mainly include the blood-heat syndrome (53.8%), blood-dryness syndrome (27.4%), and blood-stasis syndrome (18.1%).Other syndromes were rarely seen, covering 0.6%.The concurrent syndromes mainly involve dampness, heat, blood stasis and toxin.The distribution differences of the main syndromes at different stages of the disease had statistical significance (P<0.01).The syndrome distribution is not related with nationality and family history (P>0.05), but it was closely related with the psoriasis history, smoking history, alcohol drinking history, and severity of the disease (P<0.01).Conclusion:At the initial stage, psoriasis vulgaris usually manifests itself as the blood-heat syndrome, and later it may be improved or turn into the blood-dryness or blood-stasis syndrome.Smoking, alcohol consumption, and severity of the disease may play a role in the syndrome's transformation.

  14. Lupus vulgaris with squamous cell carcinoma.

    Science.gov (United States)

    Motswaledi, Mojakgomo Hendrick; Doman, Chantal

    2007-12-01

    Tuberculosis is still a significant problem in developing countries. Cutaneous forms of tuberculosis account for approximately 10% of all cases of extrapulmonary tuberculosis. Cutaneous tuberculosis may be because of true infection with Mycobacterium tuberculosis or because of tuberculids. Tuberculids are immunological reactions to haematogenously spread antigenic components of M. tuberculosis. True cutaneous tuberculosis may be because of inoculation or haematogenous spread of M. tuberculosis to the skin. Lupus vulgaris is the commonest form of true cutaneous tuberculosis. Other forms of true cutaneous tuberculosis are tuberculous chancre, tuberculosis verrucosa cutis, scrofuloderma, periorificial tuberculosis and miliary tuberculosis of the skin. Lupus vulgaris is usually chronic and progressive. It occurs in patients with moderate to high immunity against M. tuberculosis as evidenced by strongly positive tuberculin test. Long-standing cases of lupus vulgaris may be complicated by squamous cell carcinoma (SCC). We describe a patient who had undiagnosed lupus vulgaris for 35 years until she developed SCC on the lesion of lupus vulgaris.

  15. Differential expression patterns of non-symbiotic hemoglobins in sugar beet (Beta vulgaris ssp. vulgaris).

    Science.gov (United States)

    Leiva-Eriksson, Nélida; Pin, Pierre A; Kraft, Thomas; Dohm, Juliane C; Minoche, André E; Himmelbauer, Heinz; Bülow, Leif

    2014-04-01

    Biennial sugar beet (Beta vulgaris spp. vulgaris) is a Caryophyllidae that has adapted its growth cycle to the seasonal temperature and daylength variation of temperate regions. This is the first time a holistic study of the expression pattern of non-symbiotic hemoglobins (nsHbs) is being carried out in a member of this group and under two essential environmental conditions for flowering, namely vernalization and length of photoperiod. BvHb genes were identified by sequence homology searches against the latest draft of the sugar beet genome. Three nsHb genes (BvHb1.1, BvHb1.2 and BvHb2) and one truncated Hb gene (BvHb3) were found in the genome of sugar beet. Gene expression profiling of the nsHb genes was carried out by quantitative PCR in different organs and developmental stages, as well as during vernalization and under different photoperiods. BvHb1.1 and BvHb2 showed differential expression during vernalization as well as during long and short days. The high expression of BvHb2 indicates that it has an active role in the cell, maybe even taking over some BvHb1.2 functions, except during germination where BvHb1.2 together with BvHb1.1-both Class 1 nsHbs-are highly expressed. The unprecedented finding of a leader peptide at the N-terminus of BvHb1.1, for the first time in an nsHb from higher plants, together with its observed expression indicate that it may have a very specific role due to its suggested location in chloroplasts. Our findings open up new possibilities for research, breeding and engineering since Hbs could be more involved in plant development than previously was anticipated.

  16. Sister Carrie in China

    Institute of Scientific and Technical Information of China (English)

    殷希

    2015-01-01

    Sister Carrie has received many Chinese scholar's attention, and it has quantity relevance researches. Therefore, it is valuable to study why it is popular in China and it's education meaning for Chinese people. In addition, to analysis the domestic re-searches and find it's exist problems can help us make a new breakthrough from the study.

  17. The culture of Chlorella vulgaris with human urine in multibiological life support system experiments

    Science.gov (United States)

    Li, Ming; Liu, Hong; Tong, Ling; Fu, Yuming; He, Wenting; Hu, Enzhu; Hu, Dawei

    The Integrative Experimental System (IES) was established as a tool to evaluate the rela-tionship of the subsystems in Bioregenerative Life Support System, and Multibiological Life Support System Experiments (MLSSE) have been conducted in the IES. The IES consists of a higher plant chamber, an animal chamber and a plate photo bioreactor (PPB) which cultivated lettuce (Lactuca sativa L.), silkworm (Bombyx Mori L.) and microalgae (Chlorella vulgaris), respectively. In MLSSE, four volunteers took turns breathing the system air through a tube connected with the animal chamber periodically. According to the CO2 concentration in the IES, the automotive control system of the PPB changed the light intensity regulating the photosynthesis of Chlorella vulgaris to make CO2 /O2 in the system maintain at stable levels. Chlorella vulgaris grew with human urine by carrying certain amount of alga liquid out of the bioreactor every day with synthetic urine replenished into the system, and O2 was regenerated, at the same time human urine was purified. Results showed that this IES worked stably and Chlorella vulgaris grew well; The culture of Chlorella vulgaris could be used to keep the balance of CO2 and O2 , and the change of light intensity could control the gas composition in the IES; Microalgae culture could be used in emergency in the system, the culture of Chlorella vulgaris could recover to original state in 5 days; 15.6 ml of condensation water was obtained every day by the culture of Chlorella vulgaris; The removal efficiencies of N, P in human urine could reach to 98.2% and 99.5%.

  18. Pseudoainhum associated with Psoriasis vulgaris

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    Mrinal Gupta

    2015-10-01

    Full Text Available Pseudoainhum is the term applied to constricting bands around the digits or the limb which are either congenital or secondary to another disease. Progression of the constriction bands can lead to irreversible damage and autoamputation of the affected digit. Congenital pseudoainhum usually results due to amniotic bands or adhesions in utero while acquired pseudoainhum is usually secondary to trauma, neuropathy, systemic sclerosis or infections like leprosy. Psoriasis is a rare cause of acquired pseudoainhum with only five cases reported till date. We report a case of pseudoainhum secondary to psoriasis vulgaris in a 45 year old male which was successfully treated with topical corticosteroids and systemic methotrexate therapy.

  19. Lupus vulgaris of the auricle

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    Hojat Eftekhari

    2017-02-01

    Full Text Available Nowadays despite decrease in the tuberculosis (TB incidence around the world with standard anti tubercular treatment, atypical forms of TB is increasing due to extensive use of immunosuppressive therapy for autoimmune diseases and increasing prevalence of HIV infection, so early diagnosis of pulmonary and extra pulmonary TB in these patients is very important. Cutaneous TB could be a great imitator and confused with other granulomatous lesions. It has different morphological patterns. Lupus vulgaris (LV is the most common type of cutaneous TB which usually involves head and neck. We present herein a case of LV in auricular region without apparent systemic involvement.

  20. Vegetative propagation of Bambusa vulgaris

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    Rafael Malfitano Braga

    2017-06-01

    Full Text Available Bamboo is an important source of raw material of multiple uses. The development of simple techniques for its propagation is a practical way to enable its implementation in ownership of low technology. The present work had the objective of evaluating artisanal propagation methods for Bambusa vulgaris. Two types of propagules were tested, with buds budded or not, and three relative positions to the removal of vegetative material on the culm. The best propagule was with only one node, extracted from the lower thirds of the stem, presenting 72% of rooting. This result demonstrates its potential for seedling production of this species under low tech.

  1. Evaluating of Life Quality in Patients with Acne Vulgaris Using Generic and Specific Questionnaires

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    Reza Ghaderi

    2013-01-01

    Full Text Available Background. Acne vulgaris is a common skin disease that can adversely affect the quality of life of patients. Objective. The aim of this study was to determine the quality of life in patients with acne vulgaris. Methods. This study was carried out on 70 patients with acne vulgaris (28 males, 42 females. All the patients filled out two Persian versions of questionnaires: short form 36 (SF-36 and Dermatology Life Quality Index (DLQI. The obtained data were analyzed by using SPSS software (version 17. Results. The scores for physical functioning, social functioning, and bodily pain domains in patients were over 70%, but the scores for role physical, general health, vitality, role emotional, and mental health in patients were under 70%. Scores on the DLQI in patients with acne vulgaris ranged from 0 to 22 (mean ± SD, 8.18 ± 4.83. After comparing mean score of DLQI with respect to gender and age, it was found that the difference between the two groups was not statistically significant. Conclusion. Acne vulgaris has a significant effect on the quality of life. There was not any significant gender or age related difference in QOL.

  2. Management strategies for acne vulgaris

    Directory of Open Access Journals (Sweden)

    Whitney KM

    2011-04-01

    Full Text Available Kristen M Whitney1, Chérie M Ditre21Philadelphia College of Osteopathic Medicine, Philadelphia, PA, USA; 2Skin Enhancement Center and Cosmetic Dermatology, Department of Dermatology, University of Pennsylvania School of Medicine, Philadelphia, PA, USADate of preparation: 30th November 2010Conflicts of interest: None declaredClinical question: What are the most effective treatment(s for mild, moderate, severe, and hormonally driven acne?Results: Mild acne responds favorably to topical treatments such as benzoyl peroxide, salicylic acid, and a low-dose retinoid. Moderate acne responds well to combination therapy comprising-topical benzoyl peroxide, antibiotics, and/or retinoids, as well as oral antibiotics in refractory cases and oral contraceptive pills for female acne patients. Severe nodulocystic acne vulgaris responds best to oral isotretinoin therapy. In female patients with moderate to severe acne, facial hair, loss of scalp hair and irregular periods, polycystic ovarian syndrome should be considered and appropriate treatment with hormonal modulation given. Adjunctive procedures can also be considered for all acne patients.Implementation: Pitfalls to avoid when treating acne: treatment of acne in women of childbearing age; familiarization of all acne treatments in order to individualize management for patients; indications for specialist referral.Keywords: acne vulgaris, benzoyl peroxide, retinoids, antibiotics, light and laser therapy, photodynamic therapy, photopneumatic therapy, chemical peels

  3. PATIENT WITH VERUCCA VULGARIS RECCURENT

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    Ni Made Gita Saraswati

    2013-05-01

    Full Text Available Normal 0 false false false EN-US X-NONE X-NONE MicrosoftInternetExplorer4 The common wart or verruca vulgaris are benign lesions, elevated, firm nodules with characteristic papillomatous surface projections. This lession could appear anywhere in the body, mostly in fingers. The lesion could spread anywhere in the body, leaving a scar and also have a high incidence of reccurency. This report describe a man with verruca vulgaris recurrent. Preventive is important to prevent the reccurency, beside electrodesication as a curative therapy. /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0in 5.4pt 0in 5.4pt; mso-para-margin:0in; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;}

  4. Hypertrophic lupus vulgaris: an unusual presentation.

    Science.gov (United States)

    Jain, Vijay K; Aggarwal, Kamal; Jain, Sarika; Singh, Sunita

    2009-07-01

    Lupus vulgaris is the most common form of cutaneous tuberculosis occurring in previously sensitized individuals with a high degree of tuberculin sensitivity. Various forms including plaque, ulcerative, hypertrophic, vegetative, papular, and nodular forms have been described. A 30-year-old male patient presented with a very large hypertrophic lupus vulgaris lesion over left side of chest since 22 years. Histopathological examination showed granulomatous infiltration without caseation necrosis. The Mantoux reaction was strongly positive. Hypertrophic lupus vulgaris of such a giant size and that too at an unusual site is extremely rare and hence is being reported.

  5. Extensive keloidal healing of pemphigus vulgaris

    Directory of Open Access Journals (Sweden)

    Khanna Neena

    1997-01-01

    Full Text Available Bullae of pemphigus vulgaris heal without scarring. We here report a patient of pemphigus vulgaris whose lesions healed with a one-month history of extensive flaccid bullae and uninfected erosions on the trunk and extremities along with superficial erosions in the oral mucosa. The clinical suspicion of pemphigus vulgaris was confirmed by histopathological and immunohistological examination. Pulse therapy with monthly parenteral dexamethasone and cyclophosphamide pulse was instituted. The cutaneous lesions on healing formed extensive keloidal scars despite high dose of monthly corticosteroid therapy.

  6. Phaseolus vulgaris endornavirus 1 and Phaseolus vulgaris endornavirus 2 infecting common bean Phaseolus vulgaris genotypes show differential infection patterns between gene pools

    Science.gov (United States)

    We investigated the occurrence of two plant endornaviruses, Phaseolus vulgaris endornavirus 1 (PvEV-1) and Phaseolus vulgaris endornavirus 2 (PvEV-2), in breeding-lines, cultivars, landraces, and wild genotypes of common bean (Phaseolus vulgaris) as well as other Phaseolus species collected from two...

  7. Berberis Vulgaris and Berberine: An Update Review.

    Science.gov (United States)

    Imenshahidi, Mohsen; Hosseinzadeh, Hossein

    2016-11-01

    Berberine is an isoquinoline alkaloid present in several plants, including Coptis sp. and Berberis sp. Berberine is a customary component in Chinese medicine, and is characterized by a diversity of pharmacological effects. An extensive search in electronic databases (PubMed, Scopus, Ovid, Wiley, ProQuest, ISI, and Science Direct) were used to identify the pharmacological and clinical studies on Berberis vulgaris and berberine, during 2008 to 2015, using 'berberine' and 'Berberis vulgaris' as search words. We found more than 1200 new article studying the properties and clinical uses of berberine and B. vulgaris, for treating tumor, diabetes, cardiovascular disease, hyperlipidemia, inflammation, bacterial and viral infections, cerebral ischemia trauma, mental disease, Alzheimer disease, osteoporosis, and so on. In this article, we have updated the pharmacological effects of B. vulgaris and its active constituent, berberine. Copyright © 2016 John Wiley & Sons, Ltd.

  8. "Christian carrying goomies".

    Science.gov (United States)

    1994-01-01

    Dr. Passingan Usurup tells critics of his pragmatic approach on condom promotion that he is a Christian carrying condoms for Christ. He is head of the University of Papua New Guinea Medical Center and is credited with developing an AIDS/HIV policy for the Papua New Guinea Defence Force. The condoms were named Goomy and promoted at launching in 1992 in a blue packet under the slogan "The bond that guards." Goomy was chosen as the name because it is pidgin for rubber, chewing gum, and anything associated with rubber. Blue packets were chosen over the calls of most soldiers for a camouflage design because of its universal appeal as the color of the sea and sky and because it was the preference of women in the airlines. Once firmly ensconced in his role at the University, Usurup plans to develop a policy for students and staff and help to conduct AIDS prevention and education activities on campus. He will encourage students to test for HIV rather than highlighting the gloom and doom of infection and disease.

  9. Pulse Clarithromycin Therapy In Severe ACNE Vulgaris

    Directory of Open Access Journals (Sweden)

    Rathi Sanjay K

    2002-01-01

    Full Text Available Three patients with severe acne vulgaris, not responding with long courses of doxycycline, minocycline and erythromycin were given oral clarithromycin in pulsed regimen. The patients were given 7 days course of clarithromycin 250mg twice daily, which was repeated after a gap of 10 days. Such 3 courses were given. The lesions responded significantly. No significant side effect was noted. Pulse clarithromycin therapy seems to be a good alternative and effective tool in the management of severe acne vulgaris.

  10. Neonatal and infantile acne vulgaris: an update.

    Science.gov (United States)

    Serna-Tamayo, Cristian; Janniger, Camila K; Micali, Giuseppe; Schwartz, Robert A

    2014-07-01

    Acne may present in neonates, infants, and small children. Neonatal and infantile acne vulgaris are not considered to be rare. The presentation of acne in this patient population sometimes represents virilization and may portend later development of severe adolescent acne. Neonatal and infantile acne vulgaris must be distinguished from other cutaneous disorders seen in newborns and infants. Infantile acne tends to be more pleomorphic and inflammatory, thus requiring more vigorous therapy than neonatal acne.

  11. Pemphigus vulgaris presenting with multiple lesion morphologies.

    Science.gov (United States)

    Song, Philip In; Divito, Sherrie J; Kroshinsky, Daniela

    2014-12-14

    Pemphigus vulgaris is an uncommon intraepidermal blistering disorder that typically presents with flaccid bullae or erosions. We report a patient with pemphigus vulgaris who presented with several unusual clinical features: tense bullae with dependently layered pus, true target lesions coalescing into annular configurations, and diffuse desquamation that initially raised concern for toxic epidermal necrolysis. We discuss the differential diagnosis and implications of these morphological findings.

  12. Management of pemphigus vulgaris: challenges and solutions

    OpenAIRE

    Gregoriou S; Efthymiou O; Stefanaki C; Rigopoulos D

    2015-01-01

    Stamatis Gregoriou, Ourania Efthymiou, Christina Stefanaki, Dimitris Rigopoulos 2nd Department of Dermatology and Venereology, University of Athens Medical School, Attikon Hospital, Athens, Greece Abstract: The main objective in the treatment of pemphigus vulgaris is to control the disease, prevent relapses, and avoid adverse events associated with the prolonged use of steroids and immunosuppressive agents. Systemic corticosteroids remain the gold standard treatment for pemphigus vulgaris. A...

  13. Response of Desulfovibrio vulgaris to Alkaline Stress

    Energy Technology Data Exchange (ETDEWEB)

    Stolyar, S.; He, Q.; He, Z.; Yang, Z.; Borglin, S.E.; Joyner, D.; Huang, K.; Alm, E.; Hazen, T.C.; Zhou, J.; Wall, J.D.; Arkin, A.P.; Stahl, D.A.

    2007-11-30

    The response of exponentially growing Desulfovibrio vulgarisHildenborough to pH 10 stress was studied using oligonucleotidemicroarrays and a study set of mutants with genes suggested by microarraydata to be involved in the alkaline stress response deleted. The datashowed that the response of D. vulgaris to increased pH is generallysimilar to that of Escherichia coli but is apparently controlled byunique regulatory circuits since the alternative sigma factors (sigma Sand sigma E) contributing to this stress response in E. coli appear to beabsent in D. vulgaris. Genes previously reported to be up-regulated in E.coli were up-regulated in D. vulgaris; these genes included three ATPasegenes and a tryptophan synthase gene. Transcription of chaperone andprotease genes (encoding ATP-dependent Clp and La proteases and DnaK) wasalso elevated in D. vulgaris. As in E. coli, genes involved in flagellumsynthesis were down-regulated. The transcriptional data also identifiedregulators, distinct from sigma S and sigma E, that are likely part of aD. vulgaris Hildenborough-specific stress response system.Characterization of a study set of mutants with genes implicated inalkaline stress response deleted confirmed that there was protectiveinvolvement of the sodium/proton antiporter NhaC-2, tryptophanase A, andtwo putative regulators/histidine kinases (DVU0331 andDVU2580).

  14. Serum angiotensin converting enzyme in pemphigus vulgaris

    Directory of Open Access Journals (Sweden)

    Reza M Robati

    2014-01-01

    Full Text Available Background: Pemphigus vulgaris is an autoimmune blistering skin disease with unknown etiology. Drugs such as angiotensin-converting enzyme (ACE inhibitors may contribute in the pathogenesis of pemphigus. Objective: We plan this essay to evaluate the serum ACE level in pemphigus vulgaris patients in comparison with healthy controls to recognize its possible role in disease pathogenesis or activity. Methods: This study was planned and performed in the dermatology clinics of Shahid Beheshti University of MedicalSciences′ Hospitals between July 2010 and June 2011. Patients with new onset of pemphigus vulgaris were enrolled in our study. Control subjects were frequency-matched to cases by sex and age. Serum ACE was determined by the spectrophotometric method. Results: Thirty-four patients with pemphigus vulgaris and 35 healthy individuals were recruited in the study. No statistical significant difference was detected in the mean level of serum ACE of the two groups (t-test, P = 0.11. The mean ACE level was significantly lower in male patients compared with male controls (P = 0.04. Moreover, a significant higher serum ACE level of patients with cutaneous involvement was observed compared to patients with mucosal involvement (P = 0.02. Conclusions: Despite lack of any significant difference of serum ACE level between pemphigus and control group, the serum ACE level was considerably lower in male pemphigus vulgaris patients compared with male controls. Therefore, ACE might have some association with pemphigus vulgaris especially in male patients; however, further studies are required to confirm this association.

  15. Pemphigus vulgaris: approach to treatment.

    Science.gov (United States)

    Sinha, Animesh A; Hoffman, Melissa B; Janicke, Elise C

    2015-04-01

    The therapeutic management of pemphigus vulgaris (PV) is centered around immunosuppression, which can be generalized, as in the use of corticosteroids or steroid sparing agents, or specific, as in therapeutic blockage of autoantibody production, certain cytokines, or signaling pathways. Currently, the backbone of treatment for PV, particularly, first line therapy, remains systemic corticosteroids. Although very effective, the significant side effects of long-term corticosteroid usage are well documented. Adjunctive therapies aim to eliminate, or at least decrease, the necessary dose of corticosteroids. Specifically, azathioprine, cyclophosphamide, methotrexate, cyclosporine, mycophenolate mofetil and dapsone are now widely used in PV. Intravenous immunoglobulin (IVIG), plasmapheresis, immunoadsorption, and most recently, rituximab, are other members of the therapeutic armamentarium. However, despite the widening range of treatment options in PV, well-controlled clinical trials and consensus guidelines are lacking.

  16. Sunflower Seed and Acne Vulgaris.

    Science.gov (United States)

    Mohebbipour, Alireza; Sadeghi-Bazargani, Homayoun; Mansouri, Mona

    2015-09-01

    Regardless of the overall association between diet and acne which cannot be easily ignored, there might be an association between specific nutrients and acne development or improvement. The aim of this study was to assess the effect of dietary intake of sunflower seeds on acne severity and the pattern of acne lesions. In a randomized controlled trial, 50 patients aged 15 - 30 years old with acne vulgaris were enrolled through consecutive convenient sampling, in a dermatology clinic in Ardabil, Iran. They were randomly allocated into two trial arms. Those in the control group were asked to stop eating sunflower seeds if they did before. In the intervention group, they consumed 25 g sunflower-containing food daily for seven days. The primary outcome of interest was 10% increase/decrease in the baseline acne severity index (ASI), sustained to the end of the follow-up period on day 14. The mean ASI did not change significantly through the study period in the control group, but it increased in the sunflower group from 62 at the baseline to 86.8 after two weeks (P acne grading score (GAGS) did not significantly change in any of the groups and the difference in the change of GAGS was not significant between the groups (2.4 in the sunflower group versus 1.6 in the control group). Twenty two subjects (88%) in the sunflower group versus 9 (36%) in the control group had at least 10% increment in ASI throughout the follow-up period (P acne vulgaris; however, further evidence is needed to ban sunflower seed intake in patients with acne. Considering the observed potential negative effect in this trial, future randomized clinical trials may base their design on randomly assigning the exposed patients to give up use of sunflower seed intake.

  17. PEMANFAATAN MIKROALGA LAUT Chlorella vulgaris SUMBER DHA DAN EPA

    OpenAIRE

    2016-01-01

    Penelitian tentang mikroalga laut jenis Chlorella vulgaris telah dilakukan. Chlorella vulgaris dipilih sebagai bahan penambah gizi untuk di fortifikasi kedalam makanan . Penelitian ini bertujuan untuk mengetahui kandungan gizi dengan menganalisis kandungan DHA dan EPA. Penelitian ini dilakukan dengan mengkultur fitoplankton Chlorella vulgaris dan dipanen setelah media kultur mencapai fase Stasioner. Kemudian, dikeringkan dengan menggunakan freeze dryer, biomassa kering dianalisis kandungan DH...

  18. Management of pemphigus vulgaris during acute phase

    Directory of Open Access Journals (Sweden)

    Kar P

    2003-03-01

    Full Text Available We present our experience with 21 patients of pemphigus vulgaris seen over a period of 10 years managed in service hospitals during acute phase of the disease. Age groups of patients ranged from 25-45 years. Eighteen (85.7% were young adults, 30-40 years of age. Fifteen (71.4% were men and 6(28.6% were women. All the cases were hospitalized in ICU, till the acute phase of the disease subsided. Complete hematological profile, urinalysis, serum biochemistry and repeated bacterial cultures from the skin were carried out in all patients at the time of admission and thereafter weekly. The treatment comprised of potassium permanganate lotion bath (1:10000 and 1 framycetin gauze dressing of the denuded areas, maintenance of fluid and electrolyte balance. All suspected infections and septicemia were treated with appropriate antibiotics. The corticosteroids were usually administered as a single dose of prednisolone 1 mg/kg/day. Cyclophosphamide was given at an initial dose of 50mg/day and the dose was escalated to 100mg/day. Once the bulk of the lesions were healed, the dose of corticosteroids was gradually lowered by approximately 50% every two weeks and cyclophosphamide was continued till patients were symptomfree. Out of 21 patients receiving corticosteroids, cyclophosphamide and other supportive therapy, 20(95% had undergone clinical resolution of the disease. During follow up study 15(71.4% patients remained symptom-free and undergone clinical remission. Five patients (23.8% had relapse, out of which 4(19% remained symptom free, after subsequent treatment. There was one death (4.7% in our study.

  19. Treatment of acne vulgaris with fractional radiofrequency microneedling.

    Science.gov (United States)

    Kim, Sang Tae; Lee, Kang Hoon; Sim, Hyung Jun; Suh, Kee Suck; Jang, Min Soo

    2014-07-01

    Fractional radiofrequency microneedling is a novel radiofrequency technique that uses insulated microneedles to deliver energy to the deep dermis at the point of penetration without destruction of the epidermis. It has been used for the treatment of various dermatological conditions including wrinkles, atrophic scars and hypertrophic scars. There have been few studies evaluating the efficacy of fractional radiofrequency microneedling in the treatment of acne, and none measuring objective parameters like the number of inflammatory and non-inflammatory acne lesions or sebum excretion levels. The safety and efficacy of fractional radiofrequency microneedling in the treatment of acne vulgaris was investigated. In a prospective clinical trial, 25 patients with moderate to severe acne were treated with fractional radiofrequency microneedling. The procedure was carried out three times at 1-month intervals. Acne lesion count, subjective satisfaction score, sebum excretion level and adverse effects were assessed at baseline and at 4, 8 and 12 weeks after the first treatment as well as 4, 8 and 12 weeks after the last treatment. Number of acne lesions (inflammatory and non-inflammatory) decreased. Sebum excretion and subjective satisfaction were more favorable at every time point compared with the baseline values (P acne vulgaris.

  20. Complementary therapies for acne vulgaris.

    Science.gov (United States)

    Cao, Huijuan; Yang, Guoyan; Wang, Yuyi; Liu, Jian Ping; Smith, Caroline A; Luo, Hui; Liu, Yueming

    2015-01-19

    Acne is a chronic skin disease characterised by inflamed spots and blackheads on the face, neck, back, and chest. Cysts and scarring can also occur, especially in more severe disease. People with acne often turn to complementary and alternative medicine (CAM), such as herbal medicine, acupuncture, and dietary modifications, because of their concerns about the adverse effects of conventional medicines. However, evidence for CAM therapies has not been systematically assessed. To assess the effects and safety of any complementary therapies in people with acne vulgaris. We searched the following databases from inception up to 22 January 2014: the Cochrane Skin Group Specialised Register, the Cochrane Central Register of Controlled Trials (CENTRAL; 2014,Issue 1), MEDLINE (from 1946), Embase (from 1974), PsycINFO (from 1806), AMED (from 1985), CINAHL (from 1981), Scopus (from 1966), and a number of other databases listed in the Methods section of the review. The Cochrane CAM Field Specialised Register was searched up to May 2014. We also searched five trials registers and checked the reference lists of articles for further references to relevant trials. We included parallel-group randomised controlled trials (or the first phase data of randomised cross-over trials) of any kind of CAM, compared with no treatment, placebo, or other active therapies, in people with a diagnosis of acne vulgaris. Three authors collected data from each included trial and evaluated the methodological quality independently. They resolved disagreements by discussion and, as needed, arbitration by another author. We included 35 studies, with a total of 3227 participants. We evaluated the majority as having unclear risk of selection, attrition, reporting, detection, and other biases. Because of the clinical heterogeneity between trials and the incomplete data reporting, we could only include four trials in two meta-analyses, with two trials in each meta-analysis. The categories of CAM included

  1. Ocular involvement in pemphigus vulgaris.

    Science.gov (United States)

    Akhyani, Maryam; Keshtkar-Jafari, Alireza; Chams-Davatchi, Cheyda; Lajevardi, Vahide; Beigi, Sara; Aghazadeh, Nessa; Rayati Damavandi, Maede; Arami, Shabnam

    2014-07-01

    Pemphigus vulgaris (PV) is an autoimmune disorder affecting the skin and mucous membranes. Ocular involvement in PV has been reported but its prevalence and clinical characteristics are not well defined. This prospective cross-sectional study of 103 PV patients was designed to determine the prevalence, clinical types and epidemiological trends of ocular involvement in a population of Iranian patients with PV. Ocular involvement was present in 17 (16.5%) patients. Conjunctivitis was the most prevalent type of ocular involvement (9/17, 52.9%), followed by erosion of the palpebral conjunctiva (7/17, 41.2%). Erosion of the bulbar conjunctiva was noted in only one patient (5.9%). The most commonly reported symptoms were eye irritation (76.5%) and redness (76.5%). No significant relation was found between ocular involvement and disease activity (partial remission or relapse). Mucoid discharge was significantly more common in patients with conjunctival erosions as compared to patients with conjunctivitis (P = 0.038). We conclude that ocular involvement is not rare in PV; 16.5% of PV patients develop ocular disease independent of the disease activity and extension. Conjunctivitis is the most common type of involvement, however, palpebral conjunctival erosion is more frequent than previously realized.

  2. Transcriptomic and Proteomic Analysis of Arion vulgaris--Proteins for Probably Successful Survival Strategies?

    Science.gov (United States)

    Bulat, Tanja; Smidak, Roman; Sialana, Fernando J; Jung, Gangsoo; Rattei, Thomas; Bilban, Martin; Sattmann, Helmut; Lubec, Gert; Aradska, Jana

    2016-01-01

    The Spanish slug, Arion vulgaris, is considered one of the hundred most invasive species in Central Europe. The immense and very successful adaptation and spreading of A. vulgaris suggest that it developed highly effective mechanisms to deal with infections and natural predators. Current transcriptomic and proteomic studies on gastropods have been restricted mainly to marine and freshwater gastropods. No transcriptomic or proteomic study on A. vulgaris has been carried out so far, and in the current study, the first transcriptomic database from adult specimen of A. vulgaris is reported. To facilitate and enable proteomics in this non-model organism, a mRNA-derived protein database was constructed for protein identification. A gel-based proteomic approach was used to obtain the first generation of a comprehensive slug mantle proteome. A total of 2128 proteins were unambiguously identified; 48 proteins represent novel proteins with no significant homology in NCBI non-redundant database. Combined transcriptomic and proteomic analysis revealed an extensive repertoire of novel proteins with a role in innate immunity including many associated pattern recognition, effector proteins and cytokine-like proteins. The number and diversity in gene families encoding lectins point to a complex defense system, probably as a result of adaptation to a pathogen-rich environment. These results are providing a fundamental and important resource for subsequent studies on molluscs as well as for putative antimicrobial compounds for drug discovery and biomedical applications.

  3. Transcriptomic and Proteomic Analysis of Arion vulgaris--Proteins for Probably Successful Survival Strategies?

    Directory of Open Access Journals (Sweden)

    Tanja Bulat

    Full Text Available The Spanish slug, Arion vulgaris, is considered one of the hundred most invasive species in Central Europe. The immense and very successful adaptation and spreading of A. vulgaris suggest that it developed highly effective mechanisms to deal with infections and natural predators. Current transcriptomic and proteomic studies on gastropods have been restricted mainly to marine and freshwater gastropods. No transcriptomic or proteomic study on A. vulgaris has been carried out so far, and in the current study, the first transcriptomic database from adult specimen of A. vulgaris is reported. To facilitate and enable proteomics in this non-model organism, a mRNA-derived protein database was constructed for protein identification. A gel-based proteomic approach was used to obtain the first generation of a comprehensive slug mantle proteome. A total of 2128 proteins were unambiguously identified; 48 proteins represent novel proteins with no significant homology in NCBI non-redundant database. Combined transcriptomic and proteomic analysis revealed an extensive repertoire of novel proteins with a role in innate immunity including many associated pattern recognition, effector proteins and cytokine-like proteins. The number and diversity in gene families encoding lectins point to a complex defense system, probably as a result of adaptation to a pathogen-rich environment. These results are providing a fundamental and important resource for subsequent studies on molluscs as well as for putative antimicrobial compounds for drug discovery and biomedical applications.

  4. Twins with neonatal pemphigus vulgaris born to a mother with pemphigus vulgaris: a case report.

    Science.gov (United States)

    Itsukaichi, Mina; Takakuwa, Koichi; Yamaguchi, Masayuki; Serikawa, Takehiro; Tanaka, Kenichi; Kojima, Kinuko; Sakakibara, Seiichi; Usuda, Tohei; Matsunaga, Masamichi; Hashimoto, Tsuyoshi

    2013-01-01

    Dichorionic diamniotic twins were born at 37 weeks of gestation by cesarean section to a 34-year-old primigravid Japanese woman because the first twin was in breech presentation. The mother had been diagnosed with pemphigus vulgaris prior to her pregnancy. In addition to a high antidesmoglein 3 autoantibody titer, flaccid bullae and erosions on both of the twins' lips and in their oral cavities at 13 days of age led to the diagnosis of neonatal pemphigus vulgaris. This case highlights the need for awareness that pemphigus vulgaris may not occur immediately after birth.

  5. The adaptive genome of Desulfovibrio vulgaris Hildenborough.

    Science.gov (United States)

    Santana, Margarida; Crasnier-Mednansky, Martine

    2006-07-01

    Peculiar attributes revealed by sequencing the genome of Desulfovibrio vulgaris Hildenborough are analyzed, particularly in relation to the presence of a phosphotransferase system (PTS). The PTS is a typical bacterial carbohydrate transport system functioning via group translocation. Novel avenues for investigations are proposed emphasizing the metabolic diversity of D. vulgaris Hildenborough, especially the likely utilization of mannose-type sugars. Comparative analysis with PTS from other Gram-negative and Gram-positive bacteria indicates regulatory functions for the PTS of D. vulgaris Hildenborough, including catabolite repression and inducer exclusion. Chemotaxis towards PTS substrates is considered. Evidence suggests that this organism may not be a strict anaerobic sulfate reducer typical of the ocean, but a versatile organism capable of bidirectional transmigration and adaptation to both water and terrestrial environments.

  6. THERAPEUTIC AGENTS IN ACNE VULGARIS. I. TETRACYCLINE.

    Science.gov (United States)

    STEWART, W D; MADDIN, S; NELSON, A J; DANTO, J L

    1963-11-23

    A total of 120 consecutive patients with pustular and cystic acne vulgaris were selected for study. Patients were assigned a placebo and a tetracycline medication in a random method. Of the 53 patients who were given tetracycline, 45 showed some response, which was fair in 19 and excellent in 26. Of the 55 patients who received placebo, 24 showed no response while 31 showed some improvement. No side effects were reported. The difference in response between the two groups is statistically significant. It is concluded that administration of 250 mg. tetracycline four times daily, even for periods as short as two weeks, enhances the likelihood of improvement of cystic or pustular acne vulgaris.

  7. Photodynamic therapy of acne vulgaris.

    Science.gov (United States)

    Ershova, Ekaterina Y.; Karimova, Lubov N.; Kharnas, Sergey S.; Kuzmin, Sergey G.; Loschenov, Victor B.

    2003-06-01

    Photodynamic therapy (PDT) with topical 5-aminolevulinic acid (ALA) was tested for the treatment of acne vulgaris. Patients with acne were treated with ALA plus red light. Ten percent water solution of ALA was applied with 1,5-2 h occlusion and then 18-45 J/cm2 630 nm light was given. Bacterial endogenous porphyrins fluorescence also was used for acne therapy. Treatment control and diagnostics was realized by fluorescence spectra and fluorescence image. Light sources and diagnostic systems were used: semiconductor laser (λ=630 nm, Pmax=1W), (LPhT-630-01-BIOSPEC); LED system for PDT and diagnostics with fluorescent imager (λ=635 nm, P=2W, p=50 mW/cm2), (UFPh-630-01-BIOSPEC); high sensitivity CCD video camera with narrow-band wavelength filter (central wavelength 630 nm); laser electronic spectrum analyzer for fluorescent diagnostics and photodynamic therapy monitoring (LESA-01-BIOSPEC). Protoporphyrin IX (PP IX) and endogenous porphyrins concentrations were measured by fluorescence at wavelength, correspondingly, 700 nm and 650 nm. It was shown that topical ALA is converted into PP IX in hair follicles, sebaceous glands and acne scars. The amount of resulting PP IX is sufficient for effective PDT. There was good clinical response and considerable clearance of acne lesion. ALA-PDT also had good cosmetic effect in treatment acne scars. PDT with ALA and red light assist in opening corked pores, destroying Propionibacterium acnes and decreasing sebum secretion. PDT treatment associated with several adverse effects: oedema and/or erytema for 3-5 days after PDT, epidermal exfoliation from 5th to 10th day and slight pigmentation during 1 month after PDT. ALA-PDT is effective for acne and can be used despite several side effects.

  8. On fast carry select adders

    Science.gov (United States)

    Shamanna, M.; Whitaker, S.

    1992-01-01

    This paper presents an architecture for a high-speed carry select adder with very long bit lengths utilizing a conflict-free bypass scheme. The proposed scheme has almost half the number of transistors and is faster than a conventional carry select adder. A comparative study is also made between the proposed adder and a Manchester carry chain adder which shows that the proposed scheme has the same transistor count, without suffering any performance degradation, compared to the Manchester carry chain adder.

  9. On fast carry select adders

    Science.gov (United States)

    Shamanna, M.; Whitaker, S.

    This paper presents an architecture for a high-speed carry select adder with very long bit lengths utilizing a conflict-free bypass scheme. The proposed scheme has almost half the number of transistors and is faster than a conventional carry select adder. A comparative study is also made between the proposed adder and a Manchester carry chain adder which shows that the proposed scheme has the same transistor count, without suffering any performance degradation, compared to the Manchester carry chain adder.

  10. Association of human leukocyte antigen class I antigens in Iranian patients with pemphigus vulgaris.

    Science.gov (United States)

    Mortazavi, Hossein; Amirzargar, Ali Akbar; Esmaili, Nafiseh; Toofan, Hesam; Ehsani, Amir Hooshang; Hosseini, Seyed Hamed; Rezaei, Nima

    2013-04-01

    There are a limited number of reports indicating the role of human leukocyte antigen (HLA) class I alleles in pemphigus vulgaris. This study was designed to highlight the association of HLA class I alleles with pemphigus vulgaris in Iran. Fifty patients with pemphigus vulgaris, diagnosed based on clinical, histological and direct immunofluorescence findings were enrolled into this study. The control group consisted of 50 healthy, age- and sex-matched individuals. HLA typing of class I (A, B and C alleles) was carried out using polymerase chain reaction based on the sequence-specific primer method. This study showed the higher frequency of HLA-B*44:02 (P = 0.007), -C*04:01 (P pemphigus vulgaris was significantly lower than the controls. Regarding the linkage disequilibrium between HLA class I alleles, the HLA-A*03:01, -B*51:01, -C*16:02 haplotype (4% vs 0%, P = 0.04) is suggested to be a predisposing factor, whereas HLA-A*26:01, -B*38, -C*12:03 haplotype (0% vs 6%, P = 0.01) is suggested to be a protective factor. In conclusion, it is suggested that HLA-B*44:02, -C*04:01, -C*15:02 alleles and HLA-A*03:01, -B*51:01, -C*16:02 haplotype are susceptibility factors for development of pemphigus vulgaris in the Iranian population, while HLA-C*06:02, -C*18:01 alleles and HLA-A*26:01, -B*38, -C*12:03 haplotype may be considered as protective alleles.

  11. Primary structure of hemocyanin from Palinurus vulgaris

    NARCIS (Netherlands)

    Jekel, PA; Neuteboom, B; Beintema, JJ

    1996-01-01

    The primary structure of hemocyanin from the spiny lobster Palinurus vulgaris was determined using a mixture of at least four slightly different subunits. Heterogeneities were observed in 32 (5%) of the positions. The amino acid sequence differs at about 20% of the positions from that of subunit a o

  12. Chlorotic mottle of bean (Phaseolus vulgaris L.)

    NARCIS (Netherlands)

    Jayasinghe, W.U.

    1982-01-01

    For the past years there have been outbreaks of a disease of bean ( Phaseolus vulgaris L.) in Colombia called bean chlorotic mottle. The etiology of bean chlorotic mottle was not known, but the disease was generally believed to be incited by the same whitefly-transmitted virus that causes variegatio

  13. Ichthyosis vulgaris: the filaggrin mutation disease.

    Science.gov (United States)

    Thyssen, J P; Godoy-Gijon, E; Elias, P M

    2013-06-01

    Ichthyosis vulgaris is caused by loss-of-function mutations in the filaggrin gene (FLG) and is characterized clinically by xerosis, scaling, keratosis pilaris, palmar and plantar hyperlinearity, and a strong association with atopic disorders. According to the published studies presented in this review article, FLG mutations are observed in approximately 7·7% of Europeans and 3·0% of Asians, but appear to be infrequent in darker-skinned populations. This clinical review article provides an overview of ichthyosis vulgaris epidemiology, related disorders and pathomechanisms. Not only does ichthyosis vulgaris possess a wide clinical spectrum, recent studies suggest that carriers of FLG mutations may have a generally altered risk of developing common diseases, even beyond atopic disorders. Mechanistic studies have shown increased penetration of allergens and chemicals in filaggrin-deficient skin, and epidemiological studies have found higher levels of hand eczema, irritant contact dermatitis, nickel sensitization and serum vitamin D levels. When relevant, individuals should be informed about an increased risk of developing dermatitis when repeatedly or continuously exposed to nickel or irritants. Moreover, with our current knowledge, individuals with ichthyosis vulgaris should be protected against neonatal exposure to cats to prevent atopic dermatitis and should abstain from smoking to prevent asthma. Finally, they should be advised against excessive exposure to factors that decrease skin barrier functions and increase the risk of atopic dermatitis.

  14. Chlorotic mottle of bean (Phaseolus vulgaris L.)

    NARCIS (Netherlands)

    Jayasinghe, W.U.

    1982-01-01

    For the past years there have been outbreaks of a disease of bean ( Phaseolus vulgaris L.) in Colombia called bean chlorotic mottle. The etiology of bean chlorotic mottle was not known, but the disease was generally believed to be incited by the same whitefly-transmitted virus

  15. Filipendula vulgaris Moench teruggevonden bij Gennep

    NARCIS (Netherlands)

    Westhoff, V.; Leeuwen, v. Chr.G.

    1957-01-01

    De in Nederland als enig bekende groeiplaats van Filipendula vulgaris Moench (F. hexapetala Gilib.) nabij het Sanatorium “Zonlichtheide” te Gennep, is enkele jaren geleden ten offer gevallen aan de zandwinning, die daar in de Maasuiterwaard heeft plaatsgevonden. Onze vreugde was dan ook groot toen w

  16. Description of Phaseolus vulgaris L. aborting embryos from ethyl methanesulfonate (EMS mutagenized plants

    Directory of Open Access Journals (Sweden)

    Silué, S.

    2013-01-01

    Full Text Available The aim of this study was to describe the embryos abortion process and the inheritance of the embryos abortion trait in Phaseolus vulgaris plants deficient in seed development. These plants were isolated within the second generation of an ethyl methanesulfonate (EMS TILLING population of P. vulgaris cv. 'BAT93'. Mutant embryos show abnormalities mainly in suspensors, shoot apical meristem (SAM and cotyledons from the globular to the cotyledon stages and abort before maturity compared to those observed in wild-type samples. Mutant embryos show also hyperhydricity and contain low amount of chlorophyll. Genetic analyses of F1, F2 and F3 populations from the crosses carried out between the mutagenized plants with aborting embryos and the wild-type plants indicated that the embryo abortion phenotype is maternally inherited and controlled by a single recessive gene. These Phaseolus mutant plants with aborting embryos constitute a valuable material for plant embryogenesis studies.

  17. An Analysis of English Carrie

    Institute of Scientific and Technical Information of China (English)

    孙淑珍

    2004-01-01

    @@ Chapter Ⅰ Introduction Sitting in the rocking chair,Carrie dreams her future.This is the deep impression the novel"Sister Carrie"gives us,which is written by Theodore Dreiser(1871-1945),the great American realism writer.

  18. Mycophenolate mofetil as adjuvant in pemphigus vulgaris

    Directory of Open Access Journals (Sweden)

    Sarma Nilendu

    2007-01-01

    Full Text Available Pemphigus vulgaris (PV is a life threatening autoimmune blistering disease of skin and mucous membranes. Advent of systemic steroids has greatly reduced the mortality rate. However, steroids and adjuvant immunosuppressive therapy are nowadays frequent contributory agents of morbidity and mortality of PV. Mycophenolate mofetil (MMF has been reported to be an effective adjuvant to systemic steroids. It helps in increasing the immunosuppressive effect and minimizing the toxicities by steroid sparing effect. However, its efficacy in refractory cases of PV is not well documented. The lowest possible dose with satisfactory therapeutic efficacy and least side effects is known. We used MMF 1 g/day and systemic steroids in 3 Indian patients with pemphigus vulgaris who were resistant to systemic steroid monotherapy or combination treatment with azathioprine. In our experience, MMF offers an effective adjuvant with minimal side-effects in the treatment of resistant PV.

  19. Light scattering on chlorella vulgaris cells

    Science.gov (United States)

    Krol, Tadeusz; Zielinski, Andrzej; Witkowski, Konrad

    1992-12-01

    Laboratory measurements of light scattering on the axenic cultures of unicellular alga Chlorella vulgaris monoculture confirm the thesis of multi-level light scattering by the cell i.e., both by outer cell membrane and the internal structure of the cell, as well as by its molecular structures. In the measurements, the technique of dynamic light scattering and analysis by the regulation method was used, indicate that the light scattering phenomenon is affected by particles of sizes corresponding either to overall dimensions of the cell or to the dimensions of its internal structures. A correlation was found between the suggested sizes and the stage of physiological evolution of the culture. The measurements of 10 functions constituting the elements of the scattering matrix for an alive Chlorella vulgaris culture and cultures with internal cell structures modified by chemical and mechanical agents evidence that the internal structures of cells play an important role in the interaction of phytoplankton and light.

  20. Giant lupus vulgaris: A rare presentation.

    Science.gov (United States)

    Sacchidanand, S; Sharavana, S; Mallikarjun, M; Nataraja, H V

    2012-01-01

    Cutaneous tuberculosis continues to be an important public health problem even with the availability of highly effective anti-tuberculous drugs. It constitutes 0.1% of all cases of extrapulmonary tuberculosis. Lupus vulgaris is the most common form of cutaneous tuberculosis that occurs in previously sensitized individuals with a moderate degree of immunity against tubercle bacilli. The different types of lupus vulgaris include plaque, ulcerative, vegetative, papular and nodular, and tumor forms. A 40-year-old man presented with large multiple plaques over right upper limb, right side of chest and back, and right lower limb for the past 30 years. Histopathology showed numerous noncaseating granulomas with Langhan's type of giant cells. The Mantoux test showed strong positivity and there was excellent response to anti-tuberculous treatment. This case is being reported because of its extreme chronicity of 30 years duration, unusually large size and multiplicity of lesions.

  1. Acne vulgaris related to androgens - a review.

    Science.gov (United States)

    Khondker, L; Khan, S I

    2014-01-01

    Sebum production is stimulated by androgens and is the key in the development of acne vulgaris. Several investigators have looked for direct relationships between serum androgen levels, sebum secretion rate and the presence of acne. The presence of acne in prepubertal girls and sebum production in both sexes correlate with serum dehydroepiandrosterone sulfate (DHEAS) levels. Although increased serum androgen levels correlate with the presence of severe nodular acne in men and women, these levels are often within the normal range in mild to moderate acne. This raises the question of whether there is an increased local production of androgens within the sebaceous gland of patients with acne vulgaris that leads to increased sebum secretion.

  2. Pemphigus vulgaris: a rare cause of dysphagia.

    Science.gov (United States)

    Al-Janabi, Ali; Greenfield, Simon

    2015-10-22

    Pemphigus vulgaris is a rare autoimmune blistering disease of the skin and mucous membranes. The case reported presented unusually with dyspepsia that was not responsive to protein pump inhibitor (PPI) therapy. This progressed to severe dysphagia and odynophagia. An esophagogastroduodenoscopy showed extensive ulceration of the esophagus, and direct immunofluorescence of an esophageal biopsy showed bright intercellular staining with C3 and IgG, confirming the diagnosis of pemphigus vulgaris. Immunological remission was achieved after a number of courses of pulsed intravenous methylprednisolone and cyclophosphamide. The patient has remained in remission for 5 years, but has required regular dilation of esophageal strictures for symptom relief. During this period, a chronic lymphocytosis was incidentally noted on routine blood tests, and chronic lymphocytic leukaemia was diagnosed. It is essential to investigate PPI-resistant symptoms, dysphagia and odynophagia, as they may indicate a serious underlying cause.

  3. The psychosocial impact of acne vulgaris

    Directory of Open Access Journals (Sweden)

    Neirita Hazarika

    2016-01-01

    Full Text Available Background: Acne vulgaris causes erythematous papulopustular lesions in active stage and often leave behind residual scarring and pigmentation. Its onset in adolescence may add to the emotional and psychological challenges experienced during this period. Aims: To assess the impact of acne on the various psychosocial domains of daily life. Materials and Methods: This was a prospective, cross-sectional study done in the dermatology out-patient department of a tertiary care hospital from January to March 2015. A total of 100 consecutive, newly diagnosed patients of acne vulgaris, aged 15 years and above were included in this study. The relationship between acne vulgaris and its sequelae was analyzed with ten different domains of daily life by using dermatology life quality index (DLQI questionnaire. Results: Females (56%, 15–20 year olds (61%, facial lesions (60%, and Grade II acne (70% were most common. Acne scars were noted in 75% patients, whereas 79% cases had post-acne hyperpigmentation. Thirty-seven percent patients had DLQI scores of (6–10 interpreted as moderate effect on patient's life. Statistically significant correlation (P < 0.05 found were as follows: Physical symptoms with grade of acne; embarrassment with site and grade of acne; daily activities with grade of acne and post-acne pigmentation; choice of clothes with site of acne; social activities with gender, site and grade of acne; effect on work/study with grade of acne; interpersonal problems with site and post-acne pigmentation; sexual difficulties with grade of acne. Limitation: It was a hospital-based study with small sample size. Conclusion: Significant impact of acne and its sequelae was noted on emotions, daily activities, social activities, study/work, and interpersonal relationships. Assurance and counseling along with early treatment of acne vulgaris is important to reduce disease-related psychosocial sequelae and increase the efficacy of treatment.

  4. The psychosocial impact of acne vulgaris

    OpenAIRE

    Neirita Hazarika; M Archana

    2016-01-01

    Background: Acne vulgaris causes erythematous papulopustular lesions in active stage and often leave behind residual scarring and pigmentation. Its onset in adolescence may add to the emotional and psychological challenges experienced during this period. Aims: To assess the impact of acne on the various psychosocial domains of daily life. Materials and Methods: This was a prospective, cross-sectional study done in the dermatology out-patient department of a tertiary care hospital from January...

  5. [ACNE VULGARIS--AETIOLOGY, CLASSIFICATION, TREATMENT].

    Science.gov (United States)

    Janda, Katarzyna; Chwilkowska, Magdalena

    2014-01-01

    A spotless skin is a rarity. Both women and men have different problems related to the complexion. One of the most common problems is acne, which affects an increasing number of people of all ages. Seborrhea skin areas rich in sebaceous glands, the formation of comedones, inflammation, and scars are characteristic for this disease. The aim of the study was to discuss the causes of acne vulgaris, methods of treatment, and proper care of the skin affected by this problem.

  6. The Historic Panorama of Acne Vulgaris

    OpenAIRE

    Humyra Tabasum; Tanzeel Ahmad; Farzana Anjum; Hina Rehman

    2013-01-01

    Although acne is described in very ancient writings dating back to Eber’s Papyrus, its clear description is found after Fuch’s coined the term ‘Acne Vulgaris’ and Erasmus Wilson separated it from acne rosacea. The early treatment of acne was based upon the witchcraft. Later new therapies got evolved with the discoveries in the field of anatomy, physiology and biochemistry. The following review focuses the historical overview of acne vulgaris, highlighting persons and discoveries in medival an...

  7. The Psychosocial Impact of Acne Vulgaris

    Science.gov (United States)

    Hazarika, Neirita; Archana, M

    2016-01-01

    Background: Acne vulgaris causes erythematous papulopustular lesions in active stage and often leave behind residual scarring and pigmentation. Its onset in adolescence may add to the emotional and psychological challenges experienced during this period. Aims: To assess the impact of acne on the various psychosocial domains of daily life. Materials and Methods: This was a prospective, cross-sectional study done in the dermatology out-patient department of a tertiary care hospital from January to March 2015. A total of 100 consecutive, newly diagnosed patients of acne vulgaris, aged 15 years and above were included in this study. The relationship between acne vulgaris and its sequelae was analyzed with ten different domains of daily life by using dermatology life quality index (DLQI) questionnaire. Results: Females (56%), 15–20 year olds (61%), facial lesions (60%), and Grade II acne (70%) were most common. Acne scars were noted in 75% patients, whereas 79% cases had post-acne hyperpigmentation. Thirty-seven percent patients had DLQI scores of (6–10) interpreted as moderate effect on patient's life. Statistically significant correlation (P embarrassment with site and grade of acne; daily activities with grade of acne and post-acne pigmentation; choice of clothes with site of acne; social activities with gender, site and grade of acne; effect on work/study with grade of acne; interpersonal problems with site and post-acne pigmentation; sexual difficulties with grade of acne. Limitation: It was a hospital-based study with small sample size. Conclusion: Significant impact of acne and its sequelae was noted on emotions, daily activities, social activities, study/work, and interpersonal relationships. Assurance and counseling along with early treatment of acne vulgaris is important to reduce disease-related psychosocial sequelae and increase the efficacy of treatment. PMID:27688440

  8. Carrying Capacity:An Overview

    Institute of Scientific and Technical Information of China (English)

    Chen Shaofeng

    2004-01-01

    The concept of carrying capacity is derived from ecology, with widespread contentions of its theoretical connotations and applications in the international academic community, especially the impact of human activities on the environment.Disputes on carrying capacity have been occurring not only among biologists and ecologists, but also among mainstream economists. Based on their efforts,the author makes an attempt to describe its origin,connotations, problems, measurement, and at the same time note the latest international progress in this field.

  9. Insulin resistance in severe acne vulgaris.

    Science.gov (United States)

    Emiroğlu, Nazan; Cengiz, Fatma Pelin; Kemeriz, Funda

    2015-08-01

    Acne vulgaris is a pilosebaceous gland disease that usually affects people from puberty to young adulthood. It is seen especially on the face, neck, trunk and arms. Its severity differs from patient to patient and its pathogenesis is multifactorial. The main pathogenic factors of acne are high sebaceous gland secretion, follicular hyperproliferation, high androgen effects, propionibacterium acnes colonization and inflammation. Diet is always thought a probable reason for acne and many studies are done about acne and diet. To determine the effect of insulin resistance in severe acne vulgaris. Two hundred and forty-three acne vulgaris patients and 156 healthy controls were enrolled into the study. The blood levels of insulin and glucose were measured. Homeostasis Model Assessment (HOMA) Index was calculated. The values were compared with the control group. All of the patients were in the severe acne group according to their scores on the global acne scoring scale. While fasting blood glucose levels were not different between the groups (p > 0.05, 82.91 ±9.76 vs. 80.26 ±8.33), the fasting insulin levels were significantly higher in the patient group than in the control group (p acne.

  10. Pemfigus Vulgaris Manifestasi di Mulut dan Penatalaksanaannya

    Directory of Open Access Journals (Sweden)

    Metawati Tarmidi

    2015-11-01

    Full Text Available Pemfigus is an autoimmune disease involving the skin and mucosa and characterized by intraepidermal bullae formation. The four variations of pemphigus are pemphigus vulgaris, vegetans, foliaceus, and erythematosus. Although several clinical variants are recognized, pemphigus vulgaris is the most common form and the type most likely to exhibit oral manifestations. There are from 0,1 to 0,5 cases reported each year per 100,000 population with the highest incidence occurring in the fourth and seventh decades of life. Only rare cases have been reported in children and the elderly. Before the advent of systemic corticosteroid therapy, pemphigus vulgaris was fatal within few months to two years. A characteristic feature is the nikolsky sign which is the ability to elicit the formation of bullae by the application of firm lateral pressure on normal-appearing skin. The oral lesions often have a slow, insidious onset with symptoms present for many months before a diagnosis is made or skin lesions develop. Lesions may be found anywhere but are most common on the palate, buccal mucosa, and gingiva. Although the oral lesions are vesicobullous in nature, intact blisters are rarely seen. Diagnosis is established by biopsy and immunofluorescent studies. Titers on indirect immunofluorescence often correlate to the severity of the disease. The aim of this study is to promote and support early dental diagnosis when the prognosis is good.

  11. Vaccination targeting a surface sialidase of P. acnes: implication for new treatment of acne vulgaris.

    Directory of Open Access Journals (Sweden)

    Teruaki Nakatsuji

    Full Text Available BACKGROUND: Acne vulgaris afflicts more than fifty million people in the United State and the severity of this disorder is associated with the immune response to Propionibacterium acnes (P. acnes. Systemic therapies for acne target P. acnes using antibiotics, or target the follicle with retinoids such as isotretinoin. The latter systemic treatment is highly effective but also carries a risk of side effects including immune imbalance, hyperlipidemia, and teratogenicity. Despite substantial research into potential new therapies for this common disease, vaccines against acne vulgaris are not yet available. METHODS AND FINDINGS: Here we create an acne vaccine targeting a cell wall-anchored sialidase of P. acnes. The importance of sialidase to disease pathogenesis is shown by treatment of a human sebocyte cell line with recombinant sialidase that increased susceptibility to P. acnes cytotoxicity and adhesion. Mice immunized with sialidase elicit a detectable antibody; the anti-sialidase serum effectively neutralized the cytotoxicity of P. acnes in vitro and P. acnes-induced interleukin-8 (IL-8 production in human sebocytes. Furthermore, the sialidase-immunized mice provided protective immunity against P. acnes in vivo as this treatment blocked an increase in ear thickness and release of pro-inflammatory macrophage inflammatory protein (MIP-2 cytokine. CONCLUSIONS: Results indicated that acne vaccines open novel therapeutic avenues for acne vulgaris and other P. acnes-associated diseases.

  12. Variation in the Breeding System of Prunella vulgaris L.

    Science.gov (United States)

    Prunella vulgaris (Lamiaceae), commonly known as selfheal, is a perennial herb with a long history of use in traditional medicine. Recent studies have found that P. vulgaris possesses anti-inflammatory, antiviral and anti-bacterial properties, which may lead to increased commercial demand. To date...

  13. Oxidant/antioxidant status in obese adolescent females with acne vulgaris

    Directory of Open Access Journals (Sweden)

    Abulnaja Khalid

    2009-01-01

    Full Text Available Background and Objectives: Acne vulgaris is a distressing skin condition, which can carry with it significant psychological disability. Oxidant/antioxidant imbalance leads to increased production of free radicals, that cause many diseases. Some nutrients, along with systemic oxidative stress, have been implicated in acne vulgaris. The goal of the present study was to assess oxidant and antioxidant status in correlation with the incidence of acne vulgaris in adolescent obese females. Materials and Methods: A total of 60 adolescent females (age 16-22 years were divided into four groups (15 each as follows: The first included obese females with acne; the second included obese females without acne; the third included non obese with acne and the fourth included non obese without acne. Fasting serum Malondialdehyde (MDA, β-carotene, and Vitamins A, E, and C were measured. In addition, platelet monoamineoxidase (MAO, and erythrocyte catechol-o-methyltransferase (COMT activities were determined. Results: It was found that serum MDA was statistically significantly decreased in obese and non obese subjects with acne, as compared to those without acne ( P < 0.05, P < 0.001 respectively. In contrast, the levels of β-carotene, vitamins A, E and C and the activity of MAO were significantly decreased in the obese and non obese with acne, as against the obese and non obese without acne. Interpretation: In obese subjects, increased fat content facilitates free radical production and lipid peroxidation, as indicated by increased MDA level, which is scavenged by the antioxidant vitamins. The decreased activity of MAO may be inhibited by free radicals and this causes psychological depression in adolescents. However there were non significant changes in the activity of COMT among the studied groups. Conclusion: The nutritional factors and a weakened antioxidant defense system may interplay, to increase the risk of psychological sequelae in acne vulgaris.

  14. Naturalistic Elements in Sister Carrie

    Institute of Scientific and Technical Information of China (English)

    刘艳晖

    2007-01-01

    @@ Theodore Dreiser is considered to be a controversial writer.His first novel.Sister Carrie makes a new way of presenting re-ality.This paper discusses the naturalistic elements from the de-tailed description of the environment in that society.

  15. The possible effect of diets containing Nigella sativa and Thymus vulgaris on blood parameters and some organs structure in rabbit.

    Science.gov (United States)

    Tousson, Ehab; El-Moghazy, Mostafa; El-Atrsh, Efaf

    2011-03-01

    Because of the increasing cost of animal feed ingredients as well as the high demand, especially for the protein supplements, several efforts were carried out to use untraditional feed protein ingredients to participate in facing feed shortage problem and at the same time to decrease feeding costs. Nigella sativa (black cumin) and Thymus vulgaris are the most famous medical plants that have attracted the attention of many investigators for long time. The present study aimed to evaluate the possible effects of a partial replacement of soybean meal in control diet by Nigella sativa and/or Thymus vulgaris on the possible harmful changes in histological structure of some organs and blood parameters in growing New Zealand White rabbits. Blood constituents showed that the percentages of hemoglobin, hematocrate, the mean corpuscular hemoglobin and white blood cells (WBCs) count were significantly increased with the presence of black cumin seeds in the diets while WBCs count and the mean corpuscular volume in rabbit blood tends to decrease in Thymus vulgaris diets. Feeding diet supplemented with Nigella sativa increased the plasma total proteins, albumin, globulin, serum glutamate oxaloacetate transaminase (SGOT) and serum glutamate pyruvate transaminase (SGPT) and decreased total lipids, cholesterol and triglycerides. The use of Nigella sativa alone or either mixture with Thymus vulgaris are good supplements for growing rabbits without any adverse effect on histological structure of liver, kidney and testis in rabbits.

  16. Properties of Carry Value Transformation

    Directory of Open Access Journals (Sweden)

    Suryakanta Pal

    2012-01-01

    Full Text Available Carry Value Transformation (CVT is a model of discrete deterministic dynamical system. In the present study, it has been proved that (1 the sum of any two nonnegative integers is the same as the sum of their CVT and XOR values. (2 the number of iterations leading to either CVT=0 or XOR=0 does not exceed the maximum of the lengths of the two addenda expressed as binary strings. A similar process of addition of modified Carry Value Transformation (MCVT and XOR requires a maximum of two iterations for MCVT to be zero. (3 an equivalence relation is shown to exist on Z×Z which divides the CV table into disjoint equivalence classes.

  17. A new pyrroloquinazoline alkaloid from Linaria vulgaris.

    Science.gov (United States)

    Hua, Huiming; Cheng, Maosheng; Li, Xian; Pei, Yuehu

    2002-10-01

    A new alkaloid, 1,2,3,9-tetrahydropyrrolo(2,1-b)quinazolin-1-carboxylic acid (1), together with eight known compounds, 7-hydroxy vasicine (2), benzyl alcohol beta-D-(2'-O-beta-xylopyranosyl)glucopyranoside (3), benzyl alcohol O-beta-D-glucopyranoside (4), benzyl alcohol O-beta-D-primveroside (5), 3,5-dimethyl-4-hydroxy benzaldehyde (6), gluco-syringic acid (7), syringin (8), and liriodendrin (9), were isolated from the plants of Linaria vulgaris. Their structures were established by spectroscopic methods.

  18. The Historic Panorama of Acne Vulgaris

    Directory of Open Access Journals (Sweden)

    Humyra Tabasum

    2013-09-01

    Full Text Available Although acne is described in very ancient writings dating back to Eber’s Papyrus, its clear description is found after Fuch’s coined the term ‘Acne Vulgaris’ and Erasmus Wilson separated it from acne rosacea. The early treatment of acne was based upon the witchcraft. Later new therapies got evolved with the discoveries in the field of anatomy, physiology and biochemistry. The following review focuses the historical overview of acne vulgaris, highlighting persons and discoveries in medival and modern period.

  19. Annular lupus vulgaris mimicking tinea cruris.

    Science.gov (United States)

    Heo, Young Soo; Shin, Won Woong; Kim, Yong Ju; Song, Hae Jun; Oh, Chil Hwan

    2010-05-01

    Cutaneous tuberculosis is an infrequent form of extrapulmonary tuberculosis. It is often clinically and histopathologically confused with various cutaneous disorders. A 36-year-old man attended our clinic with slowly progressive, asymptomatic, annular skin lesions on both the thighs and buttocks for 10 years. He consulted with many physicians and was improperly treated with an oral antifungal agent for several months under the diagnosis of tinea cruris, but no resolution of his condition was observed. A diagnosis of lupus vulgaris was made based on the histopathologic examination and the polymerase chain reaction assay. Anti-tuberculosis therapy was administered and the lesions started to regress.

  20. Clinico- Pathological Study Of Ichthyosis Vulgaris

    Directory of Open Access Journals (Sweden)

    Nandy Utpal

    1997-01-01

    Full Text Available A clinico- pathological study of 28 cases of ichthyosis vulgaris appeared with in the age of 5 years. The presence of the disease since birth was also found. While most (24 patients showed a diminution of severity in summer with an aggravation during winter, 4 patients followed the opposite seasonal pattern. Only in 4 patients, fine scales in the scalp were detected. One patient showed an affection of flexures. There was also a low occurrence of palmo- planter hyperkeratosis, follicular keratosis, fissuring of hands and feet and atopy.

  1. Disseminated lupus vulgaris: a case report.

    Science.gov (United States)

    Can, Burce; Zindanci, Ilkin; Turkoglu, Zafer; Kavala, Mukaddes; Ulucay, Vasfiye; Demir, Filiz Topaloglu

    2014-01-01

    Lupus vulgaris is a secondary form of cutaneous tuberculosis which persists for years if not treated. The head and neck are the most commonly affected sites. While less frequently arms and legs, and rarely the trunk and the scalp are involved. Herein, we describe a 73-year-old man with a 5-year history of slowly growing, atrophic, some eroded and ulcerated, red-brown plaques on his forehead, nose, cheeks, ear lobes, trunk and extremites. All of his disseminated lesions healed after antituberculosis therapy.

  2. Lupus vulgaris in a young girl.

    Science.gov (United States)

    Goyal, Tarang; Varshney, Anupam; Bakshi, S K

    2013-01-01

    With the estimated global burden of TB being 8.8 million incident cases and 1.1 million deaths from TB in HIV-negative cases and additional 0.35 million deaths in HIV-associated cases,1 the total number of cutaneous TB cases ( lupus vulgaris in a young girl with rapid progression of a large plaque with hypertrophic features in the periphery. The case is unusual due to its rapid progression, unusual site and extensive giant form which have never been reported previously.

  3. Annular Lupus Vulgaris Mimicking Tinea Cruris

    Science.gov (United States)

    Heo, Young Soo; Shin, Won Woong; Kim, Yong Ju; Song, Hae Jun

    2010-01-01

    Cutaneous tuberculosis is an infrequent form of extrapulmonary tuberculosis. It is often clinically and histopathologically confused with various cutaneous disorders. A 36-year-old man attended our clinic with slowly progressive, asymptomatic, annular skin lesions on both the thighs and buttocks for 10 years. He consulted with many physicians and was improperly treated with an oral antifungal agent for several months under the diagnosis of tinea cruris, but no resolution of his condition was observed. A diagnosis of lupus vulgaris was made based on the histopathologic examination and the polymerase chain reaction assay. Anti-tuberculosis therapy was administered and the lesions started to regress. PMID:20548922

  4. Nootropic effect of meadowsweet (Filipendula vulgaris) extracts.

    Science.gov (United States)

    Shilova, I V; Suslov, N I

    2015-03-01

    The effects of the extracts of the aboveground parts of Filipendula vulgaris Moench on the behavior and memory of mice after hypoxic injury and their physical performance in the open-field test were studied using the models of hypoxia in a sealed volume, conditioned passive avoidance response (CPAR), and forced swimming with a load. The extracts improved animal resistance to hypoxia, normalized orientation and exploration activities, promoted CPAR retention after hypoxic injury, and increased physical performance. Aqueous extract of meadowsweet had the most pronounced effect that corresponded to the effect of the reference drug piracetam. These effects were probably caused by modulation of hippocampal activity.

  5. The association of acne vulgaris with diet.

    Science.gov (United States)

    Veith, Whitney B; Silverberg, Nanette B

    2011-08-01

    Acne vulgaris is a common disorder of the pilosebaceous unit affecting adolescents and adults. The rise in incidence of acne in the United States may relate to the Western diet--which is rich in dairy and refined sugars, and high in glycemic load--and to the obesity epidemic. We reviewed the literature on diet and acne. Evidence exists highlighting the association of acne and high glycemic loads, certain dairy products, and refined sugar product ingestion. Future studies are required to determine if dietary modification will reduce long-term acne burden.

  6. Hormonal treatment of acne vulgaris: an update

    Science.gov (United States)

    Elsaie, Mohamed L

    2016-01-01

    Acne vulgaris is a common skin condition associated with multiple factors. Although mostly presenting alone, it can likewise present with features of hyperandrogenism and hormonal discrepancies. Of note, hormonal therapies are indicated in severe, resistant-to-treatment cases and in those with monthly flare-ups and when standard therapeutic options are inappropriate. This article serves as an update to hormonal pathogenesis of acne, discusses the basics of endocrinal evaluation for patients with suspected hormonal acne, and provides an overview of the current hormonal treatment options in women. PMID:27621661

  7. Pemphigus vulgaris and amyotrophic lateral sclerosis

    Science.gov (United States)

    Mokhtari, Fatemeh; Matin, Marzieh; Rajati, Fatemeh

    2016-01-01

    Pemphigus vulgaris (PV) is an autoimmune bullous and erosive mucocutaneous disease. Rarely, it occurs in patients with other autoimmune disease. The relation between PV and neurological disorders is unclear and needs to be more studied. Here, we report a case of amyotrophic lateral sclerosis (ALS), followed by dermatologic involvement. Histopathological evidence and direct immunofluorescence are consistent with PV. Systemic corticosteroid and azathioprine were effective in the treatment of mucocutaneous lesions. PV seems to be accidentally associated with ALS. Expression of major histocompatibility complex Class II in autoimmune disease and production of autoantibodies have been proposed to describe the association of PV with ALS. PMID:28163728

  8. ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITY OF ETHANOLIC EXTRACT OF BETA VULGARIS LINN. ROOTS

    OpenAIRE

    Chakole, Rita; Zade, Shubhangi; Charde, Manoj

    2011-01-01

    The present study deals with evaluation of antioxidant and anti-inflammatory activity of ethanolic extract of Beta Vulgaris roots. The ethanolic extract was subjected to screen forantioxidant activity using DPPH radical scavenging method. The anti-inflammatory activity was carried out by using carageenan induced rat paw edema method. The tested extract ofdifferent dilutions in range 200 µg/ml to 1000 µg/ml shows activity in range of 4.34% to 18.55%. The extract shows prominent anti-inflamma...

  9. Chromosome identification in the Andean common bean accession G19833 (Phaseolus vulgaris L., Fabaceae

    Directory of Open Access Journals (Sweden)

    Sarah Altrock

    2011-01-01

    Full Text Available Characterization of all chromosomes of the Andean G19833 bean genotype was carried out by fluorescent in situ hybridization. Eleven single-copy genomic sequences, one for each chromosome, two BACs containing subtelomeric and pericentromeric repeats and the 5S and 45S ribosomal DNA (rDNA were used as probes. Comparison to the Mesoamerican accession BAT93 showed little divergence, except for additional 45S rDNA sites in four chromosome pairs. Altogether, the results indicated a relative karyotypic stability during the evolution of the Andean and Mesoamerican gene pools of P. vulgaris.

  10. Berberis vulgaris for cardiovascular disorders: a scoping literature review

    Directory of Open Access Journals (Sweden)

    Abdelrahman Abushouk

    2017-05-01

    Full Text Available Cardiovascular disorders are the leading cause of mortality worldwide. Berberis vulgaris (B. vulgaris is a commonly used plant in traditional medicine. In recent studies, B. vulgaris showed antiarrhythmic, antihypertensive, anticholinergic, and cardioprotective effects. We reviewed the literature to explore the possible prophylactic and therapeutic roles of B. vulgaris in cardiovascular medicine. A computer literature search was conducted to identify all relevant studies that have investigated the role of B. vulgaris in prevention or treatment of cardiovascular diseases.We also searched the citations of the retrieved articles. Using a systematic approach, we conducted a scoping review that included a total of 37 articles. Twelve studies examined the antihypertensive effects of B. vulgaris, seven studies investigated its antiarrhythmic effects, while its inotropic and cardioprotective effects were evaluated in four and eight studies, respectively. B. vulgaris showed a beneficial effect in reducing blood pressure, enhancing cardiac contractility, and protection from reperfusion injury. However, the mechanisms of these effects are still under investigation. Moreover, it could modify major risk factors for cardiovascular disorders, such as oxidative stress, hyperglycemia, and hyperlipidemia. Further studies are needed to translate these findings into effective cardiovascular medications.

  11. The epidemiology of acne vulgaris in late adolescence.

    Science.gov (United States)

    Lynn, Darren D; Umari, Tamara; Dunnick, Cory A; Dellavalle, Robert P

    2016-01-01

    Acne vulgaris is the most common skin condition affecting late adolescents across the globe. Although prior studies have evaluated epidemiologic patterns of acne vulgaris in various ethnicities and regions, adequate understanding of the worldwide burden of the disease associated with patients in their late adolescence (15-19-year olds) remains lacking. To assess the global burden of the disease associated with acne vulgaris for late adolescents (15-19-year olds) and provide an overview of the epidemiology, pathophysiology, and treatment options for acne in this population. Database summary study. Global Burden of Disease Study 2010 database. Global Burden of Disease regions comprised countries with prevalence of acne vulgaris between the ages of 15 and 19 years. Geographic region-level disability-adjusted life year rates (per 100,000 persons) associated with acne vulgaris in years 1990 through 2010. Median percentage change in disability-adjusted life year rates was estimated for each region across the specified study period. Acne vulgaris-associated disease burden exhibits global distribution and has continued to grow in prevalence over time within this population. This continued growth suggests an unmet dermatologic need worldwide for this disorder and potential opportunities for improved access and delivery of dermatologic care. Our analysis of the literature reveals numerous opportunities for enhanced patient care. To that end, we highlight some of the effective and promising treatments currently available and address important factors, such as sex, nationality, genetics, pathophysiology, and diet, as they relate to acne vulgaris in late adolescence.

  12. Genome-wide identification and characterisation of R2R3-MYB genes in sugar beet (Beta vulgaris).

    Science.gov (United States)

    Stracke, Ralf; Holtgräwe, Daniela; Schneider, Jessica; Pucker, Boas; Sörensen, Thomas Rosleff; Weisshaar, Bernd

    2014-09-25

    The R2R3-MYB genes comprise one of the largest transcription factor gene families in plants, playing regulatory roles in plant-specific developmental processes, metabolite accumulation and defense responses. Although genome-wide analysis of this gene family has been carried out in some species, the R2R3-MYB genes in Beta vulgaris ssp. vulgaris (sugar beet) as the first sequenced member of the order Caryophyllales, have not been analysed heretofore. We present a comprehensive, genome-wide analysis of the MYB genes from Beta vulgaris ssp. vulgaris (sugar beet) which is the first species of the order Caryophyllales with a sequenced genome. A total of 70 R2R3-MYB genes as well as genes encoding three other classes of MYB proteins containing multiple MYB repeats were identified and characterised with respect to structure and chromosomal organisation. Also, organ specific expression patterns were determined from RNA-seq data. The R2R3-MYB genes were functionally categorised which led to the identification of a sugar beet-specific clade with an atypical amino acid composition in the R3 domain, putatively encoding betalain regulators. The functional classification was verified by experimental confirmation of the prediction that the R2R3-MYB gene Bv_iogq encodes a flavonol regulator. This study provides the first step towards cloning and functional dissection of the role of MYB transcription factor genes in the nutritionally and evolutionarily interesting species B. vulgaris. In addition, it describes the flavonol regulator BvMYB12, being the first sugar beet R2R3-MYB with an experimentally proven function.

  13. Role of olfaction in Octopus vulgaris reproduction.

    Science.gov (United States)

    Polese, Gianluca; Bertapelle, Carla; Di Cosmo, Anna

    2015-01-01

    The olfactory system in any animal is the primary sensory system that responds to chemical stimuli emanating from a distant source. In aquatic animals "Odours" are molecules in solution that guide them to locate food, partners, nesting sites, and dangers to avoid. Fish, crustaceans and aquatic molluscs possess sensory systems that have anatomical similarities to the olfactory systems of land-based animals. Molluscs are a large group of aquatic and terrestrial animals that rely heavily on chemical communication with a generally dispersed sense of touch and chemical sensitivity. Cephalopods, the smallest class among extant marine molluscs, are predators with high visual capability and well developed vestibular, auditory, and tactile systems. Nevertheless they possess a well developed olfactory organ, but to date almost nothing is known about the mechanisms, functions and modulation of this chemosensory structure in octopods. Cephalopod brains are the largest of all invertebrate brains and across molluscs show the highest degree of centralization. The reproductive behaviour of Octopus vulgaris is under the control of a complex set of signal molecules such as neuropeptides, neurotransmitters and sex steroids that guide the behaviour from the level of individuals in evaluating mates, to stimulating or deterring copulation, to sperm-egg chemical signalling that promotes fertilization. These signals are intercepted by the olfactory organs and integrated in the olfactory lobes in the central nervous system. In this context we propose a model in which the olfactory organ and the olfactory lobe of O. vulgaris could represent the on-off switch between food intake and reproduction.

  14. Lupus vulgaris: unusual presentation on face.

    Science.gov (United States)

    Pilani, A; Vora, R V

    2014-01-01

    Lupus vulgaris is a variant of cutaneous tuberculosis. As the disease has potential to mutilate when left untreated, leaving deforming scars and disfigurement, an early diagnosis is of paramount importance. Though the common type is plaque type, rarely mutilating and vegetative forms also are found. A 28 year old female, labourer presented with progressive annular plaque over right side of cheek extending upto right lower lid and ala of nose. There were two satellite plaques near the right side of giant lesion. On diascopy apple jelly nodule was seen. There was no regional lymhadenopathy. Histopathological examination showed many granulomas in upper dermis extending to deep dermis comprising of epitheloid cells with langhans' type of giant cells, lymphocytic infiltration & focal necrosis suggestive of lupus vulgaris. The consequences of failing to make an early diagnosis can be disastrous for the patients, as the progression of the disease can lead to necrosis, destruction of bones and cartilage leading to permanent deformity. Thus it is vital for clinicians to have a high index of suspicion of such atypical forms and take biopsy samples for histological and bacteriological studies.

  15. Calcinosis cutis secondary to facial acne vulgaris: A rare complication

    Directory of Open Access Journals (Sweden)

    Srimanta Kumar Sahu

    2015-01-01

    Full Text Available Acne vulgaris is a common dermatological disease commonly affecting the adolescent and young adults. It is characterized by the presence of pleomorphic skin lesions such as comadones, papules, pustules, and nodules. The common complications are postacne hyperpigmentation and scarring causing psychological impact. Calcinosis cutis is the pathologic deposition of insoluble calcium salt in the skin and subcutaneous tissue. Calcinosis cutis following acne vulgaris is rarely reported in the literature. We report a case of calcinosis cutis in acne vulgaris in a 55-year-old man.

  16. Calcinosis cutis secondary to facial acne vulgaris: A rare complication.

    Science.gov (United States)

    Sahu, Srimanta Kumar; Gupta, Nikhil; Vohra, Suruchi

    2015-12-01

    Acne vulgaris is a common dermatological disease commonly affecting the adolescent and young adults. It is characterized by the presence of pleomorphic skin lesions such as comadones, papules, pustules, and nodules. The common complications are postacne hyperpigmentation and scarring causing psychological impact. Calcinosis cutis is the pathologic deposition of insoluble calcium salt in the skin and subcutaneous tissue. Calcinosis cutis following acne vulgaris is rarely reported in the literature. We report a case of calcinosis cutis in acne vulgaris in a 55-year-old man.

  17. Vascular cognitive impairment in Pemphigus vulgaris: a case report

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    José Ibiapina Siqueira- Neto

    Full Text Available ABSTRACT Pemphigus vulgaris is a systemic auto-immune medical condition that mainly manifests with changes in skin and vasculopathy. This is a case report of a 69-year-old male with confirmed histopathologic diagnosis of Pemphigus vulgaris presenting ulterior Cognitive Impairment, mostly in executive function. The patient was treated using steroids, immunomodulatory therapy, fluoxetine and galantamine. Neuropsychological testing and magnetic resonance (MRI were performed. This is the first report of correlational cognitive impairment with Pemphigus vulgaris in the literature. Physicians should be aware of vascular causes for cognitive impairment in patients presenting auto-immune conditions.

  18. A Rare Case of Cranial Osteomyelitis Caused by Proteus Vulgaris

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    Hakan Uslu

    2011-03-01

    Full Text Available Osteomyelitis of the calvarial bones can cause serious complications such as brain abscess, due to the close proximity to adjacent brain structures. Development of the purulent secretion in surgery and traumatic scalp injuries must be considered as a possibility of osteomyelitis possibility. Generally gram positive, rarely gram negative bacteria and mix agents, can be isolated in infection. Especially chronic pyogenic osteomyelitis agents can be isolated from chronic infections such as tuberculosis. In cranial osteomyelitis diagnosis, radiological diagnosis has a very important place together with the clinical diagnosis. However, infection can usually show late findings radiologically. In treatment, antibiotic treatment is absolutely essential as well as removal of the infected part of the bone. Due to antibiotic treatment lasting between 6-12 weeks, organizing the antibiotic protocols according to the results of culture-antibiograms, which were provided from purulent secretions, has the most important role in the success of surgical treatment. In Proteus sp. infections, for choice of suitable treatment, determination of the type of bacteria is important. For exact diagnosis, histopathological examination of the bone tissue must be carried out. In this report, a case with cranial osteomyelitis caused by Proteus vulgaris which is a gram negative bacteria causing anaerobic infections and classified in the Enterobacteriaceae family is presented. The patient was treated with surgery and appropriate antibiotics. Early recognition of this condition, planning the best treatment strategy and taking precautions to prevent complications, is mandatory for a better outcome.

  19. Propionibacterium acnes in the pathogenesis and immunotherapy of acne vulgaris.

    Science.gov (United States)

    Liu, Pei-Feng; Hsieh, Yao-Dung; Lin, Ya-Ching; Two, Aimee; Shu, Chih-Wen; Huang, Chun-Ming

    2015-01-01

    Acne vulgaris, a multi-factorial disease, is one of the most common skin diseases, affecting an estimated 80% of Americans at some point during their lives. The gram-positive and anaerobic Propionibacterium acnes (P. acnes) bacterium has been implicated in acne inflammation and pathogenesis. Therapies for acne vulgaris using antibiotics generally lack bacterial specificity, promote the generation of antibiotic-resistant bacterial strains, and cause adverse effects. Immunotherapy against P. acnes or its antigens (sialidase and CAMP factor) has been demonstrated to be effective in mice, attenuating P. acnes-induced inflammation; thus, this method may be applied to develop a potential vaccine targeting P. acnes for acne vulgaris treatment. This review summarizes reports describing the role of P. acnes in the pathogenesis of acne and various immunotherapy-based approaches targeting P. acnes, suggesting the potential effectiveness of immunotherapy for acne vulgaris as well as P. acnes-associated diseases.

  20. Plant Anticancer Agents XXXIII. Constituents of Passerina vulgaris1.

    Science.gov (United States)

    Ji-Xian, G; Handa, S S; Pezzuto, J M; Kinghorn, A D; Farnsworth, N R

    1984-06-01

    Two lignans of known structure, (+)-syringaresinol, a cytotoxic agent, and (+)-nortrachelogenin, a compound with demonstrated antileukemic activity, were isolated from a biologically active extract of the stems of PASSERINA VULGARIS.

  1. Using bamboo (Bambusa vulgaris) as a field drainage material in ...

    African Journals Online (AJOL)

    user

    Bamboo (Bambusa vulgaris), one of the most widespread member of its genus, was ... climatic conditions and soil types (United Nations, 1972). ... concrete pipes are still being used as well. .... Tropical rain Forest zone with an annual rainfall of.

  2. Phenotypic Diversity among Croatian Common Bean (Phaseolus vulgaris L.) Landraces

    National Research Council Canada - National Science Library

    Monika Vidak; Sara Malešević; Martina Grdiša; Zlatko Šatović; Boris Lazarević; Klaudija Carović-Stanko

    2016-01-01

    Phenotypic diversity among Croatian common bean (Phaseolus vulgaris L.) landraces was assessed by analysing 12 qualitative and six quantitative traits in 338 accessions collected from all production areas in Croatia...

  3. Polyphosphate during the Regreening of Chlorella vulgaris under Nitrogen Deficiency

    National Research Council Canada - National Science Library

    Chu, Fei-Fei; Shen, Xiao-Fei; Lam, Paul K S; Zeng, Raymond J

    2015-01-01

    Polyphosphate (Poly-P) accumulation has been reported in Chlorella vulgaris under nitrogen deficiency conditions with sufficient P supply, and the process has been demonstrated to have great impact on lipid productivity...

  4. [Mapping of pathogenic genes in two families with autosomal dominant ichthyosis vulgaris].

    Science.gov (United States)

    Gong, Hui-Yong; Zhang, Jing; Hu, Zheng-Mao; Wu, Ling-Qian; Liang, De-Sheng; Xie, Zhi-Guo; Pan, Qian; Bu, Feng-Xiao; Peng, Yu; Xia, Kun; Xia, Jia-Hui

    2008-07-01

    To localize the pathogenic genes of autosomal dominant ichthyosis vulgaris, we ascertained two ichthyosis vulgaris families from Hunan Province. Venous blood samples were collected from affected and unaffected family members and genomic DNA was extracted. We then performed genome scan and linkage analysis using microsatellite markers around known ichthyosis vulgaris loci in chromosomes 1 and 10. In family 1, the locus linked to ichthyosis vulgaris was located near D1S498 (1q21), which overlapped with known ichthyosis vulgaris loci. In family 2, however, all known loci for ichthyosis vulgaris were excluded and the new locus remains to be identified.

  5. Potential Health Effects of Enzymatic Protein Hydrolysates from Chlorella vulgaris

    OpenAIRE

    2016-01-01

    Background and Objective: Chlorella vulgaris is a multi-cellular edible algal species with abundant proteins. Extraction of high value protein fractions for pharmaceutical and nutritional applications can significantly increase the commercial value of microalga biomasses. There is no known report on the anticancer peptides derived from the Chlorella vulgaris abundant protein.Materials and Methods: This study examined the antimicrobial and anticancer effects of peptides from a hydrolyzed Chlor...

  6. Ichthyosis Vulgaris Coexisted with Acrokeratosis Verruciformis: A Case Report

    Directory of Open Access Journals (Sweden)

    Gamze Serarslan

    2008-12-01

    Full Text Available Ichthyosis vulgaris is an autosomal dominant inherited, keratinization disorder and characterized by diffuse scaling. Acrokeratosis verruciformis is also an autosomal dominant, rare keratinization disorder and characterized by warty, brownish to skin colored papules on the dorsa of the hands and feet. We present a case of ichthyosis vulgaris coexisted with acrokeratosis verruciformis in a 24-year-old woman. (Turkderm 2008; 42: 134-6

  7. Lupus vulgaris of external nose--a case report.

    Science.gov (United States)

    Arunkumar, J S; Naveen, K N; Prasad, K C; Santhosh, S G; Hegde, J S

    2013-02-01

    Lupus vulgaris is the most common morphological variant of cutaneous tuberculosis accounting for approximately 59% of cases of cutaneous tuberculosis in India. We present a case of lupus vulgaris of external nose diagnosed early and treated with CAT-3 RNTCP regimen for six months without any nasal deformity except for a small scar over the dorsum of the nose. Patient followed up for one year after completion of the prescribed regimen, there being no recurrence of the lesion.

  8. Effects of processing on bean (Phaseolus vulgaris L.) protein quality.

    OpenAIRE

    Poel, van der, A.F.B.

    1990-01-01

    In animal production, feeding has an important impact on productivity and health of animals and feed composition is known to influence protein and energy metabolism directly. For monogastric animals complete diets are manufactured in which feed ingredients are used to supply the energy yielding and other nutrients. The common bean ( Phaseolus vulgaris L.) is such an ingredient.In common beans ( Phaseolus vulgaris L.) the supply of nutrients is often lower than is expected from its chemical an...

  9. Influence of diet in acne vulgaris and atopic dermatitis.

    Science.gov (United States)

    Shokeen, Divya

    2016-09-01

    Diet has been considered as an influence in dermatology for several years. Unfortunately, although correlation has been breached, causation is yet to be determined. Over the last couple years, a few reviews of the literature have been published regarding the influence of diet in acne vulgaris and atopic dermatitis. This article reviews some dietary restrictions and supplements that may have beneficial effects in managing patients with acne vulgaris and atopic dermatitis.

  10. Chlorella vulgaris: A Multifunctional Dietary Supplement with Diverse Medicinal Properties.

    Science.gov (United States)

    Panahi, Yunes; Darvishi, Behrad; Jowzi, Narges; Beiraghdar, Fatemeh; Sahebkar, Amirhossein

    2016-01-01

    Chlorella vulgaris is a green unicellular microalgae with biological and pharmacological properties important for human health. C. vulgaris has a long history of use as a food source and contains a unique and diverse composition of functional macro- and micro-nutrients including proteinsChlorella vulgaris is a green unicellular microalgae with biological and pharmacological properties important for human health. C. vulgaris has a long history of use as a food source and contains a unique and diverse composition of functional macro- and micro-nutrients including proteins, omega-3 polyunsaturated fatty acids, polysaccharides, vitamins and minerals. Clinical trials have suggested that supplementation with C. vulgaris can ameliorate amelioration hyperlipidemia and hyperglycemia, and protect against oxidative stress, cancer and chronic obstructive pulmonary disease. In this review, we summarize the findings on the health benefits of Chlorella supplementation and the molecular mechanisms underlying these effects., omega-3 polyunsaturated fatty acids, polysaccharides, vitamins and minerals. Clinical trials have suggested that supplementation with C. vulgaris can ameliorate amelioration hyperlipidemia and hyperglycemia, and protect against oxidative stress, cancer and chronic obstructive pulmonary disease. In this review, we summarize the findings on the health benefits of Chlorella supplementation and the molecular mechanisms underlying these effects.

  11. Essential Oil Characterization of Thymus vulgaris from Various Geographical Locations.

    Science.gov (United States)

    Satyal, Prabodh; Murray, Brittney L; McFeeters, Robert L; Setzer, William N

    2016-10-27

    Thyme (Thymus vulgaris L.) is a commonly used flavoring agent and medicinal herb. Several chemotypes of thyme, based on essential oil compositions, have been established, including (1) linalool; (2) borneol; (3) geraniol; (4) sabinene hydrate; (5) thymol; (6) carvacrol, as well as a number of multiple-component chemotypes. In this work, two different T. vulgaris essential oils were obtained from France and two were obtained from Serbia. The chemical compositions were determined using gas chromatography-mass spectrometry. In addition, chiral gas chromatography was used to determine the enantiomeric compositions of several monoterpenoid components. The T. vulgaris oil from Nyons, France was of the linalool chemotype (linalool, 76.2%; linalyl acetate, 14.3%); the oil sample from Jablanicki, Serbia was of the geraniol chemotype (geraniol, 59.8%; geranyl acetate, 16.7%); the sample from Pomoravje District, Serbia was of the sabinene hydrate chemotype (cis-sabinene hydrate, 30.8%; trans-sabinene hydrate, 5.0%); and the essential oil from Richerenches, France was of the thymol chemotype (thymol, 47.1%; p-cymene, 20.1%). A cluster analysis based on the compositions of these essential oils as well as 81 additional T. vulgaris essential oils reported in the literature revealed 20 different chemotypes. This work represents the first chiral analysis of T. vulgaris monoterpenoids and a comprehensive description of the different chemotypes of T. vulgaris.

  12. Essential Oil Characterization of Thymus vulgaris from Various Geographical Locations

    Directory of Open Access Journals (Sweden)

    Prabodh Satyal

    2016-10-01

    Full Text Available Thyme (Thymus vulgaris L. is a commonly used flavoring agent and medicinal herb. Several chemotypes of thyme, based on essential oil compositions, have been established, including (1 linalool; (2 borneol; (3 geraniol; (4 sabinene hydrate; (5 thymol; (6 carvacrol, as well as a number of multiple-component chemotypes. In this work, two different T. vulgaris essential oils were obtained from France and two were obtained from Serbia. The chemical compositions were determined using gas chromatography–mass spectrometry. In addition, chiral gas chromatography was used to determine the enantiomeric compositions of several monoterpenoid components. The T. vulgaris oil from Nyons, France was of the linalool chemotype (linalool, 76.2%; linalyl acetate, 14.3%; the oil sample from Jablanicki, Serbia was of the geraniol chemotype (geraniol, 59.8%; geranyl acetate, 16.7%; the sample from Pomoravje District, Serbia was of the sabinene hydrate chemotype (cis-sabinene hydrate, 30.8%; trans-sabinene hydrate, 5.0%; and the essential oil from Richerenches, France was of the thymol chemotype (thymol, 47.1%; p-cymene, 20.1%. A cluster analysis based on the compositions of these essential oils as well as 81 additional T. vulgaris essential oils reported in the literature revealed 20 different chemotypes. This work represents the first chiral analysis of T. vulgaris monoterpenoids and a comprehensive description of the different chemotypes of T. vulgaris.

  13. Essential Oil Characterization of Thymus vulgaris from Various Geographical Locations

    Science.gov (United States)

    Satyal, Prabodh; Murray, Brittney L.; McFeeters, Robert L.; Setzer, William N.

    2016-01-01

    Thyme (Thymus vulgaris L.) is a commonly used flavoring agent and medicinal herb. Several chemotypes of thyme, based on essential oil compositions, have been established, including (1) linalool; (2) borneol; (3) geraniol; (4) sabinene hydrate; (5) thymol; (6) carvacrol, as well as a number of multiple-component chemotypes. In this work, two different T. vulgaris essential oils were obtained from France and two were obtained from Serbia. The chemical compositions were determined using gas chromatography–mass spectrometry. In addition, chiral gas chromatography was used to determine the enantiomeric compositions of several monoterpenoid components. The T. vulgaris oil from Nyons, France was of the linalool chemotype (linalool, 76.2%; linalyl acetate, 14.3%); the oil sample from Jablanicki, Serbia was of the geraniol chemotype (geraniol, 59.8%; geranyl acetate, 16.7%); the sample from Pomoravje District, Serbia was of the sabinene hydrate chemotype (cis-sabinene hydrate, 30.8%; trans-sabinene hydrate, 5.0%); and the essential oil from Richerenches, France was of the thymol chemotype (thymol, 47.1%; p-cymene, 20.1%). A cluster analysis based on the compositions of these essential oils as well as 81 additional T. vulgaris essential oils reported in the literature revealed 20 different chemotypes. This work represents the first chiral analysis of T. vulgaris monoterpenoids and a comprehensive description of the different chemotypes of T. vulgaris. PMID:28231164

  14. Changes in serum desnutrin levels in patients with acne vulgaris.

    Science.gov (United States)

    Demir, Betul; Ucak, Haydar; Cicek, Demet; Aydin, Suleyman; Erden, Ilker; Dertlioglu, Selma Bakar

    2014-01-01

    Androgens and insulin may contribute to increased sebum production in the pathogenesis of acne vulgaris. We investigated the association between serum desnutrin levels and acne vulgaris in the pathogenesis of insulin resistance. 25 patients presenting with acne vulgaris and 25 control subjects participated in this study. Fasting blood glucose, triglycerides, LDL, VLDL, HDL, total cholesterol, insulin, C-peptide and thyroid function tests were measured. The homeostasis model assessment of insulin resistance (HOMA-IR) was used to calculate insulin resistance. Desnutrin levels were determined by enzyme-linked immunosorbent assay (ELISA) according to the manufacturer's protocol. Patients with acne vulgaris had a mean serum desnutrin level of (8.83 ± 1.13 μIU/mL), which was statistically significantly lower in the control group (10:58 ± 3.43 μIU/mL). In patients with acne vulgaris the serum glucose levels, insulin levels and HOMA-IR values (87.92 ± 7:46 mg/dL, 11.33 ± 5.93 μIU/mL, 2.49 ± 1.40, respectively) were significantly higher than the control group (77.36 ± 9.83 mg/dL, 5.82 ± 2.68 μIU/mL, 1.11 ± 0.51, respectively) (p = 0.01, pacne vulgaris, as a result of increased levels of serum glucose and insulin, the function of desnutrin was suppressed, perhaps contributing to insulin resistance.

  15. Pemphigus Vulgaris and Infections: A Retrospective Study on 155 Patients

    Directory of Open Access Journals (Sweden)

    Nafiseh Esmaili

    2013-01-01

    Full Text Available Background. Autoimmune process and immunosuppressive therapy of pemphigus vulgaris would predispose the patients to infections. Aim. We aimed to study the prevalence of infection and pathogenic agents in pemphigus vulgaris patients admitted to dermatology service. Material and methods. This retrospective study was conducted on 155 pemphigus vulgaris patients (68 males, 87 females admitted to dermatology service between 2009 and 2011. In this study, the diagnosis of pemphigus vulgaris was confirmed by light microscopic and direct immunofluorescence findings. Data were collected through a questionnaire. Results. Of 155 pemphigus vulgaris patients, 33 had infection at admission and 9 acquired nosocomial infection. In addition, 37 cases of oral candidiasis and 15 cases of localized herpes simplex were recorded. Totally, 94 cases of infection were recorded. The occurrence of infection was significantly related to the severity of disease, number of hospital admissions, and presence of diabetes mellitus. The most common pathogenic germs isolated from cultures were Staphylococcus aureus and Escherichia coli. Conclusion. Severity of pemphigus vulgaris and diabetes were directly related with tendency to infections. Staphylococcus aureus and Escherichia coli were the most common pathogenic agents. Due to limitations of retrospective study, a prospective study is recommended.

  16. Acne vulgaris and rosacea: evaluation and management.

    Science.gov (United States)

    Webster, G F

    2001-01-01

    Acne vulgaris, commonly termed acne, is an extremely common disease. It can be found in nearly all teenagers to some degree as well as in women in their 30s. Regardless of severity, acne often has a greater psychologic effect than cutaneous effect. Indeed, most patients overestimate the severity of their disease, while most physicians underestimate its impact on their patients. Studies have shown that people with severe acne as teens are less employable as adults and that self-esteem is low. When combined with other adolescent tensions, acne can be a difficult disease to treat. Rosacea, which usually starts in the late 20s, may affect the eyes as well as the skin. This article describes the pathogenesis of acne and rosacea and treatment approaches the primary care physician can use.

  17. Ichthyosis vulgaris and pycnodysostosis: an unusual occurrence.

    Science.gov (United States)

    Kshirsagar, Vinayak Y; Ahmed, Minhajuddin; Nagarsenkar, Suhel; Sahoo, Kulmani; Shah, Kuldeep B

    2012-01-01

    Pycnodysostosis is a rare autosomal recessive disorder whose gene responsible for this phenotype (CTSK), mapped to human chromosome 1q21, code for the enzyme cathepsin K, a lysosomal cysteine protease; with an estimated incidence of 1.7 per 1 million births. This clinical entity includes micromelic dwarfism, increased radiological bone density, dysplasia of the skull, acro-osteolysis, straightening of the mandibular angle and in some cases, dysplasia of the acromial end of the clavicle. Oral and maxillo-facial manifestations of this disease are very clear. Herein we reported a case of pycnodysostosis, showing short stature with widening of the sutures, unfused anterior and posterior fontanelles, crowding of teeth with dental caries and typical radiological features associated with ichthyosis vulgaris and palmoplantar keratoderma.

  18. Pemphigus vulgaris: a multidisciplinary approach to management.

    Science.gov (United States)

    Vinall, Christopher; Stevens, Lucy; McArdle, Paul

    2013-12-16

    Pemphigus vulgaris (PV) is a rare but potentially life-threatening autoimmune disease affecting the mucosa and the skin. The disease is caused by circulating antibodies to desmosomes (important adhesion proteins linking cells together). Disruption of these intercellular connections results in a loss of cohesion between cells (acantholysis). The clinical result of this process is the development of multiple blisters that easily rupture, leaving behind painful sloughing eroded areas of mucosa and/or skin. We report a case of severe PV in a 56-year-old man presenting with widespread, painful, eroded mucocutaneous lesions. The severity of the disease demanded a range of medical and surgical specialties to successfully manage the problem. This paper highlights the importance of an early multidisciplinary team approach to improve the outcome of patients suffering with this disease.

  19. Plastid transformation in sugar beet: Beta vulgaris.

    Science.gov (United States)

    De Marchis, Francesca; Bellucci, Michele

    2014-01-01

    Chloroplast biotechnology has assumed great importance in the past 20 years and, thanks to the numerous advantages as compared to conventional transgenic technologies, has been applied in an increasing number of plant species but still very much limited. Hence, it is of utmost importance to extend the range of species in which plastid transformation can be applied. Sugar beet (Beta vulgaris L.) is an important industrial crop of the temperate zone in which chloroplast DNA is not transmitted trough pollen. Transformation of the sugar beet genome is performed in several research laboratories; conversely sugar beet plastome genetic transformation is far away from being considered a routine technique. We describe here a method to obtain transplastomic sugar beet plants trough biolistic transformation. The availability of sugar beet transplastomic plants should avoid the risk of gene flow between these cultivated genetic modified sugar beet plants and the wild-type plants or relative wild species.

  20. Pemphigus vulgaris-associated interstitial lung disease.

    Science.gov (United States)

    Bai, Yi-Xiu; Chu, Jin-Gang; Xiao, Ting; Chen, Hong-Duo

    2016-07-01

    Autoimmune bullous diseases (AIBDs)-associated interstitial lung disease (ILD) is extremely rare. Pemphigus vulgaris (PV) is an intraepidermal autoimmune blistering disease caused by circulating autoantibodies against desmoglein. To date, PV-associated ILD has rarely been reported in English literature. We report a rare association of PV and ILD. A 53-year-old Chinese female with PV for 8 months developed ILD after a relapse of PV for 2 months due to discontinuation of oral prednisone by herself. She was successfully treated by systemic methylprednisolone. Taken previously reported bullous pemphigoid-associated ILD and linear IgA/IgG bullous dermatosis-associated ILD together, in general, AIBDs-associated ILD occurs when AIBDs relapse or are not controlled, responds well to systemic corticosteroids, and has a relatively better prognosis when compared with rheumatoid arthritis- or dermatomyositis-associated ILD.

  1. Pregnancy-Associated "Cutaneous Type" Pemphigus Vulgaris.

    Science.gov (United States)

    Rangel, Javier

    2016-01-01

    The development of pemphigus, including pemphigus vulgaris (PV) and pemphigus foliaceus, during pregnancy is rare. PV manifests with mucosal and/or cutaneous erosions with flaccid bullae that are histologically characterized by suprabasilar acantholysis. In contrast, pemphigus foliaceus manifests with cutaneous-only involvement and superficial epidermal acantholysis. Enzyme-linked immunosorbent assay specific for autoantibodies against desmoglein 1 and desmoglein 3 aids in the diagnosis and differentiation between pemphigus subtypes. High-dose systemic corticosteroids are first-line agents in management of PV, yet their potential long-term use raises complex management issues associated with pregnancy and fetal risk. Here we report a rare case of cutaneous-limited PV in association with pregnancy.

  2. Setting the target for pemphigus vulgaris therapy

    Science.gov (United States)

    Ellebrecht, Christoph T.

    2017-01-01

    Despite the rising incidence of autoimmunity, therapeutic options for patients with autoimmune disease still rely on decades-old immunosuppressive strategies that risk severe and potentially fatal complications. Thus, novel therapeutic approaches for autoimmune diseases are greatly needed in order to minimize treatment-related toxicity. Such strategies would ideally target only the autoreactive immune components to preserve beneficial immunity. Here, we review how several decades of basic, translational, and clinical research on the immunology of pemphigus vulgaris (PV), an autoantibody-mediated skin disease, have enabled the development of targeted immunotherapeutic strategies. We discuss research to elucidate the pathophysiology of PV and how the knowledge afforded by these studies has led to the preclinical and clinical testing of targeted approaches to neutralize autoantibodies, to induce antigen-specific tolerance, and to specifically eliminate autoreactive B cells in PV. PMID:28289723

  3. Ichthyosis vulgaris and pycnodysostosis: An unusual occurrence

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    Vinayak Y. Kshirsagar

    2012-11-01

    Full Text Available Pycnodysostosis is a rare autosomal recessive disorder whose generesponsible for this phenotype (CTSK, mapped to human chromosome1q21, code for the enzyme cathepsin K, a lysosomal cysteineprotease; with an estimated incidence of 1.7 per 1 million births. This clinical entity includes micromelic dwarfism, increased radiological bone density, dysplasia of the skull, acro-osteolysis, straightening of the mandibular angle and in some cases, dysplasia of the acromial end of the clavicle. Oral and maxillo-facial manifestations of this disease are very clear. Herein we reported a case of pycnodysostosis, showing short stature with widening of the sutures, unfused anterior and posterior fontanelles, crowding of teeth with dental caries and typical radiological features associated with ichthyosis vulgaris and palmoplantar keratoderma.

  4. Single and mixture toxicity of pharmaceuticals and chlorophenols to freshwater algae Chlorella vulgaris.

    Science.gov (United States)

    Geiger, Elisabeth; Hornek-Gausterer, Romana; Saçan, Melek Türker

    2016-07-01

    Organisms in the aquatic environment are exposed to a variety of substances of numerous chemical classes. The unintentional co-occurrence of pharmaceuticals and other contaminants of emerging concern may pose risk to non-target organisms. In this study, individual and binary mixture toxicity experiments of selected pharmaceuticals (ibuprofen and ciprofloxacin) and chlorophenols (2.4-dichlorophenol (2,4-DCP) and 3-chlorophenol (3-CP)) have been performed with freshwater algae Chlorella vulgaris. All experiments have been carried out according to the 96-h algal growth inhibition test OECD No. 201. Binary mixture tests were conducted using proportions of the respective IC50s in terms of toxic unit (TU). The mixture concentration-response curve was compared to predicted effects based on both the concentration addition (CA) and the independent action (IA) model. Additionally, the Combination Index (CI)-isobologram equation method was used to assess toxicological interactions of the binary mixtures. All substances individually tested had a significant effect on C. vulgaris population density and revealed IC50 values ciprofloxacin>3-CP>ibuprofen. Generally, it can be concluded from this study that toxic mixture effects of all tested chemicals to C. vulgaris are higher than the individual effect of each mixture component. It could be demonstrated that IC50 values of the tested mixtures predominately lead to additive effects. The CA model is appropriate to estimate mixture toxicity, while the IA model tends to underestimate the joint effect. The CI-isobologram equation method predicted the mixtures accurately and elicited synergism at low effect levels for the majority of tested combinations.

  5. Genetics, chemistry and ecology of a qualitative glucosinolate polymorphism in Barbarea vulgaris

    NARCIS (Netherlands)

    Van Leur, H.

    2008-01-01

    Like many other plants, chemical defence compounds are involved in the defense of Barbarea vulgaris against natural enemies. Barbarea vulgaris produces glucosinolates, which are present in most crucifers such as cabbage, mustard, and the scientific model species Arabidopsis thaliana. Glucosinolates

  6. Genetics, chemistry and ecology of a qualitative glucosinolate polymorphism in Barbarea vulgaris

    NARCIS (Netherlands)

    Van Leur, H.

    2008-01-01

    Like many other plants, chemical defence compounds are involved in the defense of Barbarea vulgaris against natural enemies. Barbarea vulgaris produces glucosinolates, which are present in most crucifers such as cabbage, mustard, and the scientific model species Arabidopsis thaliana. Glucosinolates

  7. Infections That Pets Carry (For Parents)

    Science.gov (United States)

    ... Your 1- to 2-Year-Old Infections That Pets Carry KidsHealth > For Parents > Infections That Pets Carry ... how to protect your family from infections. How Pets Spread Infections Like people, all animals carry germs . ...

  8. Salt Stress in Desulfovibrio vulgaris Hildenborough: An integratedgenomics approach

    Energy Technology Data Exchange (ETDEWEB)

    Mukhopadhyay, Aindrila; He, Zhili; Alm, Eric J.; Arkin, Adam P.; Baidoo, Edward E.; Borglin, Sharon C.; Chen, Wenqiong; Hazen, Terry C.; He, Qiang; Holman, Hoi-Ying; Huang, Katherine; Huang, Rick; Hoyner,Dominique C.; Katz, Natalie; Keller, Martin; Oeller, Paul; Redding,Alyssa; Sun, Jun; Wall, Judy; Wei, Jing; Yang, Zamin; Yen, Huei-Che; Zhou, Jizhong; Keasling Jay D.

    2005-12-08

    The ability of Desulfovibrio vulgaris Hildenborough to reduce, and therefore contain, toxic and radioactive metal waste has made all factors that affect the physiology of this organism of great interest. Increased salinity is an important and frequent fluctuation faced by D. vulgaris in its natural habitat. In liquid culture, exposure to excess salt resulted in striking elongation of D. vulgaris cells. Using data from transcriptomics, proteomics, metabolite assays, phospholipid fatty acid profiling, and electron microscopy, we used a systems approach to explore the effects of excess NaCl on D. vulgaris. In this study we demonstrated that import of osmoprotectants, such as glycine betaine and ectoine, is the primary mechanism used by D. vulgaris to counter hyperionic stress. Several efflux systems were also highly up-regulated, as was the ATP synthesis pathway. Increases in the levels of both RNA and DNA helicases suggested that salt stress affected the stability of nucleic acid base pairing. An overall increase in the level of branched fatty acids indicated that there were changes in cell wall fluidity. The immediate response to salt stress included up-regulation of chemotaxis genes, although flagellar biosynthesis was down-regulated. Other down-regulated systems included lactate uptake permeases and ABC transport systems. The results of an extensive NaCl stress analysis were compared with microarray data from a KCl stress analysis, and unlike many other bacteria, D. vulgaris responded similarly to the two stresses. Integration of data from multiple methods allowed us to develop a conceptual model for the salt stress response in D. vulgaris that can be compared to those in other microorganisms.

  9. Variability of cadmium accumulation in cephalopods (Octopus vulgaris, Sepia officinalis, Loligo vulgaris and Todarodes sagittatus collected in Sardinia in 2008-2012

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    Pierluigi Piras

    2013-06-01

    Full Text Available Cephalopods are known to accumulate cadmium and play an important role in its biomagnification. They are an essential link in marine trophic chain and represent an important case in studies on cadmium transfer to man through the food chain. Since cadmium concentration widely varies in different tissues of the cephalopods – mainly accumulating in the hepatopancreas – evisceration represents a recommended preliminary step to reduce cadmium intake in view of cephalopods consumption; yet, the residual concentration in the edible part may still be a risk for public health. This study is intended to assess cadmium levels variability in the muscles of Cephalopoda, considering the different feeding habitats and marine trophic webs. In compliance with EU regulation, a survey on cadmium and other heavy metal levels in various sea food, including cephalopods, was conducted by the Istituto Zooprofilattico of Sardinia in co-operation with local health authorities. During a five-year survey (2008- 2012, 90 samples were collected from the following species: commons octopus (Octopus vulgaris, cuttlefish (Sepia officinalis, European squid (Loligo vulgaris, and flying squid (Todarodes sagittatus, located in different coastal areas and representatives of either benthic or nektonic habitats. Determination of cadmium levels was carried out according to Regulations (EC No. 882/2004, No. 1881/2006 and No. 333/2007. Analysis of the edible portion (muscle of fresh homogenised samples was carried out by inductively coupled plasma mass spectrometry (ICP-MS. The results showed a highly skewed distribution of data. No statistically significant differences were observed among four distributions of the natural logs of cadmium levels in the species considered.

  10. Biofilm growth mode promotes maximum carrying capacity and community stability during product inhibition syntrophy.

    Science.gov (United States)

    Brileya, Kristen A; Camilleri, Laura B; Zane, Grant M; Wall, Judy D; Fields, Matthew W

    2014-01-01

    Sulfate-reducing bacteria (SRB) can interact syntrophically with other community members in the absence of sulfate, and interactions with hydrogen-consuming methanogens are beneficial when these archaea consume potentially inhibitory H2 produced by the SRB. A dual continuous culture approach was used to characterize population structure within a syntrophic biofilm formed by the SRB Desulfovibrio vulgaris Hildenborough and the methanogenic archaeum Methanococcus maripaludis. Under the tested conditions, monocultures of D. vulgaris formed thin, stable biofilms, but monoculture M. maripaludis did not. Microscopy of intact syntrophic biofilm confirmed that D. vulgaris formed a scaffold for the biofilm, while intermediate and steady-state images revealed that M. maripaludis joined the biofilm later, likely in response to H2 produced by the SRB. Close interactions in structured biofilm allowed efficient transfer of H2 to M. maripaludis, and H2 was only detected in cocultures with a mutant SRB that was deficient in biofilm formation (ΔpilA). M. maripaludis produced more carbohydrate (uronic acid, hexose, and pentose) as a monoculture compared to total coculture biofilm, and this suggested an altered carbon flux during syntrophy. The syntrophic biofilm was structured into ridges (∼300 × 50 μm) and models predicted lactate limitation at ∼50 μm biofilm depth. The biofilm had structure that likely facilitated mass transfer of H2 and lactate, yet maximized biomass with a more even population composition (number of each organism) when compared to the bulk-phase community. Total biomass protein was equivalent in lactate-limited and lactate-excess conditions when a biofilm was present, but in the absence of biofilm, total biomass protein was significantly reduced. The results suggest that multispecies biofilms create an environment conducive to resource sharing, resulting in increased biomass retention, or carrying capacity, for cooperative populations.

  11. Biofilm growth mode promotes maximum carrying capacity and community stability during product inhibition syntrophy

    Directory of Open Access Journals (Sweden)

    Kristen Annis Brileya

    2014-12-01

    Full Text Available Sulfate-reducing bacteria (SRB can interact syntrophically with other community members in the absence of sulfate, and interactions with hydrogen-consuming methanogens are beneficial when these archaea consume potentially inhibitory H2 produced by the SRB. A dual continuous culture approach was used to characterize population structure within a syntrophic biofilm formed by the SRB Desulfovibrio vulgaris Hildenborough and the methanogenic archaeum Methanococcus maripaludis. Under the tested conditions, monocultures of D. vulgaris formed thin, stable biofilms, but monoculture M. maripaludis did not. Microscopy of intact syntrophic biofilm confirmed that D. vulgaris formed a scaffold for the biofilm, while intermediate and steady-state images revealed that M. maripaludis joined the biofilm later, likely in response to H2 produced by the SRB. Close interactions in structured biofilm allowed efficient transfer of H2 to M. maripaludis, and H2 was only detected in cocultures with a mutant SRB that was deficient in biofilm formation ( delta pilA. M. maripaludis produced more carbohydrate (uronic acid, hexose, and pentose as a monoculture compared to total coculture biofilm, and this suggested an altered carbon flux during syntrophy. The syntrophic biofilm was structured into ridges (~300 x 50 um and models predicted lactate limitation at approximately 50 um biofilm depth. The biofilm had structure that likely facilitated mass transfer of H2 and lactate, yet maximized biomass with a more even population composition (number of each organism when compared to the bulk-phase community. Total biomass protein was equivalent in lactate-limited and lactate-excess conditions when a biofilm was present, but in the absence of biofilm, total biomass protein was significantly reduced. The results suggest that multispecies biofilms create an environment conducive to resource sharing, resulting in increased biomass retention, or carrying capacity, for cooperative

  12. Changes in Botrytis cinerea Conidia Caused by Berberis vulgaris Extract

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    Marcel PARVU

    2010-12-01

    Full Text Available Testing plant extracts for controlling fungal diseases is a main biocontrol method. More interesting is to see what happens to the fungus treated with the plant extract. Therefore, the aim of the study was to evaluate the antifungal activity of Berberis vulgaris extract on Botrytis cinerea and to examine the ultrastructural changes in B. cinerea conidia caused by the minimum inhibitory concentration (MIC, using SEM and TEM. The antifungal activity of B. vulgaris bark extract was investigated using agar dilution method, and compared to that of berberine. Fluconazole was used as the positive antimycotic control. It was found that (1 B. vulgaris bark extract had significant antifungal activity against B. cinerea, and its effect was stronger than that of pure berberine. It was also noted that (2B. vulgaris MIC caused severe structural changes of the conidia, comparable with berberine MIC effect; therefore (3 B. vulgaris bark extract might be recommended to be tested as a biocontrol agent against B. cinerea.

  13. [Lupus vulgaris manifestation as a destructive nose and facial tumor].

    Science.gov (United States)

    Haller, D; Reisser, C

    2009-04-01

    Lupus vulgaris is the most frequent manifestation of cutaneous tuberculosis, but in Europe it is limited to isolated cases. Mainly immunocompetent individuals are affected by this result of an endogenous reinfection on a lymphogenous-less frequently hematogenous-pathway. Lupus vulgaris has been observed to develop in more than 50% of all patients who already suffer from other manifestations of tuberculosis. The development of a squamous cell carcinoma in the lupus vulgaris is a rare complication; therefore, lupus vulgaris is deemed a facultative precancerosis.A 68-year-old female Serbo-Croatian patient presented with an extensive ulcerative nose and facial tumor. Her anamnesis included a squamous cell carcinoma of the nose that had been excised alio loco 3 years before. Further examinations revealed enlarged cervical lymphoma on both sides, and pulmonary metastases were also suspected. The tumor biopsy revealed a necrotic, granulomatous inflammation. No acid-fast rods were seen on Ziehl-Neelsen stain. The tuberculous origin of this ulcerative skin tumor-the lupus vulgaris-as an endogenous reinfection of pulmonary tuberculosis manifestation was confirmed by the detection of Mycobacterium tuberculosis DNA in polymerase chain reaction and the growth of Mycobacterium tuberculosis colonies in the bacterial culture (skin biopsy and bronchial secretion). The skin tumor as well as the pulmonary manifestation were successfully treated with combined tuberculostatic therapy and showed a dramatic response within 3 months.

  14. Acne vulgaris: diagnosis and management by the family physician

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    Ana Margarida Ferreira da Silva

    2014-01-01

    Full Text Available Acne vulgaris is the most common skin disease worldwide. It affects 85-100% of the population at any point in life. Consequently, it is a frequent reason for primary care visits. It usually begins at puberty, reaching its peak between the age of 14-17 in girls, and 16-19 in boys, and it is more severe and prevalent in males. Although widely discussed, Acne vulgaris still requires constant updating. We conducted a survey of clinical guidelines, systematic reviews, and meta-analyses published over the past 15 years, concerning acne vulgaris and its treatment. Acne vulgaris is usually divided in three clinical types: comedonal, papulopustular, and nodular. The diagnosis is based on clinical signs; however, other conditions should be considered. There are several pharmacological therapies available, especially retinoids, antimicrobials, and hormone therapy, which should be used after considering its indications (type of acne and its severity and side effects. Thus, the family physician plays a leading role in addressing acne vulgaris, from diagnosis to management of therapeutic options. 

  15. Gender-based variability in disease presentation in pemphigus vulgaris.

    Science.gov (United States)

    Naseer, Sahar Y; Gill, Liza; Shah, Jay; Sinha, Animesh A

    2014-10-01

    Pemphigus vulgaris is a chronic autoimmune blistering disorder of the skin. As with many autoimmune diseases, a female predominance in pemphigus vulgaris is well established. The genetic and physiological basis for this gender bias is not well understood. Moreover, it is unclear whether the affect of gender extends beyond disease susceptibility to influence disease presentation. To address this issue, we performed a comprehensive analysis of 72 male and 125 female pemphigus vulgaris patients across a set of defined demographic (HLA type, ethnicity) and clinical (age at disease onset, anti-desmoglein antibody levels, site of lesions, and history of autoimmune disease) factors. We find that male patients are more likely to present with disease onset before age 40 than females. Additionally, we find that males have increased cutaneous involvement and display greater co-expression of anti-Dsg1 and anti-Dsg3 antibodies, while females tend to have mucosal predominance and stronger personal and family histories of autoimmunity. We do not find any differences in the distribution of HLA type or ethnicity between male and female pemphigus vulgaris patients. Our findings establish that gender does influence disease presentation in pemphigus vulgaris, supporting a role for genetic and hormonal factors in immune dysregulation and perpetuation of the autoimmune phenotype.

  16. A Brief Analysis of Sister Carrie's Character

    Science.gov (United States)

    Yu, Hanying

    2010-01-01

    Carrie is always dreaming while the rocking chair is rocking again and again, this is the deep impression on us after we read "Sister Carrie" which is the first novel of Theodore Dreiser. In this novel the protagonist Sister Carrie is a controversial person. This paper tries to analyze the character of Sister Carrie in order to find out…

  17. Methylcobalamin--a form of vitamin B12 identified and characterised in Chlorella vulgaris.

    Science.gov (United States)

    Kumudha, Anantharajappa; Selvakumar, Sagaya; Dilshad, Pullancheri; Vaidyanathan, Gopal; Thakur, Munna Singh; Sarada, Ravi

    2015-03-01

    Vitamin B12 is among the most essential biomolecules required for crucial metabolic processes in humans. Vitamin B12 was extracted from Chlorella vulgaris biomass under aqueous conditions, partially purified by passing the extract through amberlite XAD-2, Sep-Pak columns, and further purified by HPLC. The target peak eluent was subjected to characterisation by tandem mass spectrometry (MS/MS), selected ion recording (SIR) and multiple reaction monitoring (MRM) and identified as methylcobalamin (Me-Cbl). Quantification of Me-Cbl was carried out by microbiological and chemiluminescence methods, and found to be 29.87±2 μg/100 g and 26.84±2 μg/100 g dry weight, respectively. The presence of Me-Cbl was further substantiated using gold nanoparticle (AuNPs) based aptamer analysis, and found to be 28.02±2 μg/100 g dry weight. Good similarity was observed among all the methods. Methylcobalamin, a form of vitamin B12 was identified in C. vulgaris and this finding enhances its use as a nutritional supplement.

  18. Histochemistry and infrared microspectroscopy of lignified tissue in young stems of Struthanthus vulgaris Mart.

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    Gisely de Lima Oliveira

    Full Text Available In this study, we aimed to determine lignified tissue in young stems of Struthanthus vulgaris Mart. by infrared microspectroscopy and histochemical methods as well as by fluorescence microscopy. Struthanthus vulgaris Mart. is a mistletoe species that belongs to the Loranthaceae family. A brief anatomical description was also carried out. The first procedure for analysis was to elaborate anatomical cross sections (20-30 µm from young stems before and after treatment with NaOH 1%. This procedure was applied to release possible low molecular mass phenolic compounds. Safranin-astra blue was used to distinguish anatomical tissues while Wiesner test enabled verification of lignified pericyclic fibers. Infrared microspectroscopy analysis confirmed the presence of lignin in this region according to the following spectral signals: 1600 (shoulder, 1511, 1453, 1338 and 1244 cm-1. Analyses of the cross section of young stems under fluorescence microscopy before and after treatment with NaOH 1% allowed us to confirm the presence of low mass phenolic compounds in the region of pericyclic fibers.

  19. Characteristics and kinetics study of simultaneous pyrolysis of microalgae Chlorella vulgaris, wood and polypropylene through TGA.

    Science.gov (United States)

    Azizi, Kolsoom; Keshavarz Moraveji, Mostafa; Abedini Najafabadi, Hamed

    2017-11-01

    Thermal decomposition behavior and kinetics of microalgae Chlorella vulgaris, wood and polypropylene were investigated using thermogravimetric analysis (TGA). Experiments were carried out at heating rates of 10, 20 and 40°C/min from ambient temperature to 600°C. The results show that pyrolysis process of C. vulgaris and wood can be divided into three stages while pyrolysis of polypropylene occurs almost totally in one step. It is shown that wood can delay the pyrolysis of microalgae while microalgae can accelerate the pyrolysis of wood. The existence of polymer during the pyrolysis of microalgae or wood will lead to two divided groups of peaks in DTG curve of mixtures. The results showed that interaction is inhibitive rather than synergistic during the decomposition process of materials. Kinetics of process is studied by the Kissinger-Akahira-Sunose (KAS) and Flynn-Wall-Ozawa (FWO). The average E values obtained from FWO and KAS methods were 131.228 and 142.678kJ/mol, respectively. Copyright © 2017 Elsevier Ltd. All rights reserved.

  20. Topical erythromycin-zinc acetate complex lotion versus topical erythromycin gel in treatment of mild to moderate acne vulgaris

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    Hayder R. Al-Hamamy

    2014-10-01

    Full Text Available Introduction: Topical antibiotics are the main step in the treatment of mild to moderate acne vulgaris. Erythromycin is one of the effective topical therapies for this disease. Zinc sulfate 5% solution was reported to be effective in treatment of acne vulgaris and rosacea. Aim: To compare the effectiveness and side effects of topical erythromycin in combination with zinc and erythromycin alone in treatment of mild to moderate acne vulgaris. Material and Methods: This single, blind, therapeutic, comparative study was done in the Department of Dermatology – Baghdad Teaching Hospital, Baghdad, Iraq; from May 2012 – August 2013.Scoring of acne was carried out and the patients were examined every two weeks for 10 weeks of treatment. One month after stopping drugs, patients were evaluated for drug complications and disease recurrence. Eighty patients fulfilling enrollment criteria were included in this study. Patients were divided into two groups: Group A (40 patients treated twice daily with topical erythromycin-zinc complex lotion and Group B (40 patients treated twice daily with topical 2% erythromycin gel. Results: Both topical erythromycin-zinc lotion and erythromycin gel were statistically an effective therapy starting after 6 weeks treatment and up to 4 weeks after stopping treatment. Erythromycin-zinc lotion was more effective and act earlier than erythromycin gel starting from 4 weeks of therapy till the end of treatment (after 10 week and even after 4 weeks after stopping the treatment (p value <0.0001. Conclusions: Erythromycin-zinc complex lotion was an effective and well tolerated topical therapy for mild to moderate inflammatory acne vulgaris and was more effective than erythromycin gel alone.

  1. The complete chloroplast genome sequence of sugar beet (Beta vulgaris ssp. vulgaris).

    Science.gov (United States)

    Li, Han; Cao, Hua; Cai, Yan-Fei; Wang, Ji-Hua; Qu, Su-Ping; Huang, Xing-Qi

    2014-06-01

    The complete nucleotide sequence of the sugar beet (Beta vulgaris ssp. vulgaris) chloroplast genome (cpDNA) was determined in this study. The cpDNA was 149,637 bp in length, containing a pair of 24,439 bp inverted repeat regions (IR), which were separated by small and large single copy regions (SSC and LSC) of 17,701 and 83,057 bp, respectively. 53.4% of the sugar beet cpDNA consisted of gene coding regions (protein coding and RNA genes). The gene content and relative positions of 113 individual genes (79 protein encoding genes, 30 tRNA genes, 4 rRNA genes) were almost identical to those of tobacco cpDNA. The overall AT contents of the sugar beet cpDNA were 63.6% and in the LSC, SSC and IR regions were 65.9%, 70.8% and 57.8%, respectively. Fifteen genes contained one intron, while three genes had two introns.

  2. Treatment of acne vulgaris in pregnant patients.

    Science.gov (United States)

    Pugashetti, Rupa; Shinkai, Kanade

    2013-01-01

    The management of acne vulgaris in the setting of pregnancy raises important clinical considerations regarding the efficacy and safety of acne treatments in this special patient population. Particular challenges include the absence of safety data, discrepancy in safety data between different safety rating systems, and lack of evidence-based recommendations for the treatment of acne during pregnancy. Nonetheless, many therapeutic options exist, and the treatment of acne in pregnant women can be safely and often effectively accomplished. For mild or moderate disease, patients can be treated with topical antimicrobial agents, anti-inflammatory agents, as well as glycolic and salicylic acid. Several topical agents, notably benzoyl peroxide, previously viewed as potentially dangerous are cited by many sources as being considered safe. When necessary, systemic therapies that can be safely added include penicillins, amoxicillin, cephalosporins, erythromycin, clindamycin, and tetracyclines or sulfonamides, depending on the stage of fetal development. Adjunct therapy may include phototherapy or laser treatments. Physicians should work with this often highly motivated, safety-conscious patient population to tailor an individualized treatment regimen. This treatment regimen will likely shift throughout the different stages of fetal development, as distinct safety considerations are raised prior to conception as well as during each of the trimesters of pregnancy. Important considerations regarding acne management in breast-feeding mothers is also discussed.

  3. Topical and oral antibiotics for acne vulgaris.

    Science.gov (United States)

    Del Rosso, James Q

    2016-06-01

    Antibiotics, both oral and topical, have been an integral component of the management of acne vulgaris (AV) for approximately 6 decades. Originally thought to be effective for AV due to their ability to inhibit proliferation of Propionibacterium acnes, it is now believed that at least some antibiotics also exert anti-inflammatory effects that provide additional therapeutic benefit. To add, an increase in strains of P acnes and other exposed bacteria that are less sensitive to antibiotics used to treat AV have emerged, with resistance directly correlated geographically with the magnitude of antibiotic use. Although antibiotics still remain part of the therapeutic armamentarium for AV treatment, current recommendations support the following when used to treat AV: 1) monotherapy use should be avoided; 2) use benzoyl peroxide concomitantly to reduce emergence of resistant P acnes strains; 3) oral antibiotics should be used in combination with a topical regimen for moderate-to-severe inflammatory AV; and 4) use oral antibiotics over a limited duration to achieve control of inflammatory AV with an exit plan in place to discontinue their use as soon as possible. When selecting an oral antibiotic to treat AV, potential adverse effects are important to consider.

  4. Impaired water barrier function in acne vulgaris.

    Science.gov (United States)

    Yamamoto, A; Takenouchi, K; Ito, M

    1995-01-01

    In acne vulgaris, abnormal follicular keratinization is important for comedo formation, yet the precise mechanisms of comedogenesis are not known. The present study examined the interrelationship between sebum secretion rate (SSR), lipid content and water barrier function (WBF) of the stratum corneum (SC) in 36 acne patients and 29 control subjects. All major SC lipid classes were separated and quantified by thin-layer chromatography/photodensitometry. WBF was evaluated by measuring transepidermal water loss (TEWL), and the hygroscopic properties and waterholding capacity of the SC. The SSR over a period of 3 h was significantly higher in patients with moderate acne than in control subjects, but no significant difference was noticed between patients with mild acne and control subjects. Significant differences between patients with both moderate and mild acne and control subjects were noted in the amount of sphingolipids (ceramides and free sphingosine), but not for any other lipid classes. Furthermore in acne patients, lower amounts of sphingolipids were observed corresponding with a diminished WBF. These results suggest that an impaired WBF caused by decreased amounts of ceramides may be responsible for comedo formation, since barrier dysfunction is accompanied by hyperkeratosis of the follicular epithelium.

  5. Acne vulgaris in children and adolescents.

    Science.gov (United States)

    Schnopp, C; Mempel, M

    2011-08-01

    Acne vulgaris is a very common inflammatory skin disease originating from the pilosebaceous unit. Peak incidence is at puberty, but acne can affect all age groups. Prepubertal acne is rare, but important to recognize as diagnostic and therapeutic procedures differ from pubertal acne. Acne neonatorum is a mild, self-limiting disease, whereas acne infantum commonly presents with moderate to severe lesions and high risk of scarring thus requiring early intervention. Mid-childhood or prepubertal acne raises the suspicion of hyperandrogenemia, further investigations are indicated to rule out underlying disease. The same applies to any patient with very severe acne, acne not responding to therapy or unusual clinical presentation. Etiopathogenesis of acne is not yet fully understood. Familiy history is the most important risk factor to develop severe acne and scarring. The relevance of life style factors such as smoking or diet is controversial. Lately high carbohydrate diet and dairy products have been implicated as aggravating factors. Mild acne normally responds to topical monotherapy, in moderate disease combination of two synergistically acting substances (e.g. benzoyl peroxid plus antibiotic, benzoyl peroxid plus retinoid, retinoid plus antibiotic, benzoyl peroxid plus azelaic acid) will improve clinical response. Retinoids and/or benzoylperoxid have been shown to be effective in maintenance therapy. In patients with severe disease or high risk of scarring systemic therapy with antibiotics, oral contraceptives with antiandrogenic properties and in particularly isotretinoin as most effective acne treatment should be considered early to avoid physical and emotional scars.

  6. Lupus vulgaris in a pediatric patient: a clinicohistopathological diagnosis.

    Science.gov (United States)

    Afsar, F Sule; Afsar, Ilhan; Diniz, Gulden; Asilsoy, Suna; Sorguc, Yelda

    2008-04-01

    Lupus vulgaris is the most common form of cutaneous tuberculosis which usually occurs in patients previously sensitized to Mycobacterium tuberculosis. We present a case of a 10-year-old boy who was diagnosed as lupus vulgaris clinically and histopathologically. He had well demarcated, irregularly bordered, pink, infiltrated plaques on his left cheek showing apple-jelly appearance on diascopy. The histopathological examination showed tuberculoid granulomas with Langhans type giant cells. The Mantoux reactivity was in normal limits, and no acid-fast bacilli was found in the lesion, either by direct stained smears or by culture. The lesions showed marked improvement on anti-tuberculosis treatment. We want to emphasize that histopathological examination has diagnostic value in lupus vulgaris in correlation with clinical appearance, when direct analysis or culture is negative.

  7. Lupus vulgaris occurring in a locus minoris resistentiae.

    Science.gov (United States)

    Long, Richard; Beatch, Anita; Lee, Mao-Cheng; Cheung-Lee, Melody; Wasel, Norman

    2009-01-01

    The pathogenesis of lupus vulgaris, a form of cutaneous tuberculosis, is not always clear, especially in patients who do not have coexistent extracutaneous tuberculosis and in patients with single lesions. To report a case of lupus vulgaris in a locus minoris resistentiae (a site of reduced resistance) and to use a unique set of clinical circumstances and laboratory tests to reconstruct the pathogenesis of the lesion and the response to treatment. Lupus vulgaris can occur in a locus minoris resistentiae; local trauma and possibly other factors, such as increased temperature, topical corticosteroids, and the virulence of the infecting strain, may facilitate the growth of Mycobacterium tuberculosis present at a locus minoris resistentiae as a result of a silent bacillemia.

  8. Lupus vulgaris in a pediatric patient: a clinicohistopathological diagnosis

    Directory of Open Access Journals (Sweden)

    F. Sule Afsar

    2008-04-01

    Full Text Available Lupus vulgaris is the most common form of cutaneous tuberculosis which usually occurs in patients previously sensitized to Mycobacterium tuberculosis. We present a case of a 10-year-old boy who was diagnosed as lupus vulgaris clinically and histopathologically. He had well demarcated, irregularly bordered, pink, infiltrated plaques on his left cheek showing apple-jelly appearance on diascopy. The histopathological examination showed tuberculoid granulomas with Langhans type giant cells. The Mantoux reactivity was in normal limits, and no acid-fast bacilli was found in the lesion, either by direct stained smears or by culture. The lesions showed marked improvement on anti-tuberculosis treatment. We want to emphasize that histopathological examination has diagnostic value in lupus vulgaris in correlation with clinical appearance, when direct analysis or culture is negative.

  9. The innate immunity in the cnidarian Hydra vulgaris

    Directory of Open Access Journals (Sweden)

    B Altincicek

    2009-08-01

    Full Text Available Hydra vulgaris is currently receiving increased attention as a genetically tractable invertebrate model system for studying important processes of life such as the innate immune defense. Similar to complex animals, H. vulgaris polyps respond to injury by abrupt muscle contraction, by limited escape behavior, and by healing the damaged tissue. Simultaneously, cellular processes such as phagocytosis and programmed cell death as well as the massive production of antimicrobial peptides are induced. Recent studies identified several molecular pathways controlling these responses; however, the interdependence of innate immunity and, for example, regeneration and tissue remodeling is not well elucidated yet. H. vulgaris belongs to the Cnidaria representing the phylogenic sister group of bilaterian animals; hence, a better understanding of evolutionarily conserved as well as Hydra/Cnidaria-specific immune responses will provide deep insight into both origin and evolution of the animal innate immune system

  10. Recycling of food waste as nutrients in Chlorella vulgaris cultivation.

    Science.gov (United States)

    Lau, Kin Yan; Pleissner, Daniel; Lin, Carol Sze Ki

    2014-10-01

    Heterotrophic cultivation of Chlorella vulgaris was investigated in food waste hydrolysate. The highest exponential growth rate in terms of biomass of 0.8day(-1) was obtained in a hydrolysate consisting of 17.9gL(-1) glucose, 0.1gL(-1) free amino nitrogen, 0.3gL(-1) phosphate and 4.8mgL(-1) nitrate, while the growth rate was reduced in higher concentrated hydrolysates. C. vulgaris utilized the nutrients recovered from food waste for the formation of biomass and 0.9g biomass was produced per gram glucose consumed. The microalgal biomass produced in nutrient sufficient batch cultures consisted of around 400mgg(-1) carbohydrates, 200mgg(-1) proteins and 200mgg(-1) lipids. The conversion of nutrients derived from food waste and the balanced biomass composition make C. vulgaris a promising strain for the recycling of food waste in food, feed and fuel productions.

  11. Timely recognition of pemphigus vulgaris by dental professionals.

    Science.gov (United States)

    Fatahzadeh, Mahnaz

    2013-07-01

    Pemphigus vulgaris is a potentially fatal mucocutaneous, vesiculobullous disorder of autoimmune etiology. Regrettably, affected patients frequently experience considerable discomfort and diagnostic delay for months in spite of multiple clinician contacts. The high likelihood of disease manifestations in the oral cavity and serious nature of potential oral and systemic complications mandates dental professionals to recognize early oral signs and symptoms of pemphigus vulgaris and contribute to timely diagnosis and medical intervention to prevent disease progression. This case report presents a young man with pemphigus vulgaris whose oral disease caused him significant suffering and spread to the skin before he was finally diagnosed 3 months after the onset. The signs and symptoms of the disease are reviewed and the potential role of dental providers in timely recognition and management is emphasized.

  12. Autoreactive T cells in the immune pathogenesis of pemphigus vulgaris.

    Science.gov (United States)

    Amber, Kyle T; Staropoli, Patrick; Shiman, Michael I; Elgart, George W; Hertl, Michael

    2013-11-01

    Pemphigus vulgaris is a life-threatening autoimmune blistering disease caused by anti-desmoglein IgG autoantibodies that finally lead to acantholysis presenting clinically as progressive blistering. Whilst the production of pathogenic antibodies is key to the development of pemphigus vulgaris, many immunological steps are required prior to autoantibody induction. We review advances in the understanding of these immunologic processes with a focus on human leucocyte antigen polymorphisms and antigen recognition, epitope spreading, central and peripheral tolerance, T helper differentiation, induction of pro- and anti-inflammatory cytokines and T-cell regulation of B cells. Targeting autoaggressive T cells as regulators and stimulators of B-cell antibody production should allow for more specific therapeutic immune interventions, avoiding the global immunosuppression seen with many commonly used immunosuppressants in pemphigus vulgaris.

  13. Respuesta de Phaseolus vulgaris a microorganismos promotores de crecimiento vegetal

    Directory of Open Access Journals (Sweden)

    Violeta Edith Romero - García

    2016-01-01

    Full Text Available El objetivo de esta investigaci ón fue analizar la respuesta de P. vulgaris a la inoculación individual y consorcio de MPCV a dosis reducida al 50% del FN. Para ello se inoculó P. vulgaris individualmente con: B. cereus, R. etli y T. harzianum y en consorcio de M PCV en jarras de Leonard tratada c on NH 4 NO 3 al 50 %, bajo un diseño experimental de bloques al azar; con variables respuestas: porcentaje y días a germinación, altura de planta y longitud radical, peso fresco/seco aéreo y radical (PFA/PSA/PFR/PSR a plántula y floración; los datos experimentales se analizaron por Tukey , p ≤ 0 , 05. Los resultados mostraron que P. vulgaris inoculado con R. etli y T. harzianum tuvo un 100 % y 95,8% de germinación o emergencia en 4,8 y 4 , 5 días, respectivamente, valores con diferencia estadística en comparación con el 91,7% en 7,17 días en P. vu lgaris sin inocular con el 100% de FN o testigo fertilizado (TF. En la etapa de plántula de P. vulgaris con T. harzianum y R. etli fue de 1,12 g y 0,72 g PSA, así como de 0,31 g y 0,21 de PSR, respectivamente , superior a P. vulgaris (TF de 0,52 g de PSA y 0,19 g de PSR. Lo anterior apoya el potencial de R. etli y T. harzianum para la producción de P. vulgaris a dosis reducida de FN, comparado con el efecto positivo individual de B. cereus y en consorcio en la misma leguminosa .

  14. Transcriptome analysis of the Octopus vulgaris central nervous system.

    Directory of Open Access Journals (Sweden)

    Xiang Zhang

    Full Text Available BACKGROUND: Cephalopoda are a class of Mollusca species found in all the world's oceans. They are an important model organism in neurobiology. Unfortunately, the lack of neuronal molecular sequences, such as ESTs, transcriptomic or genomic information, has limited the development of molecular neurobiology research in this unique model organism. RESULTS: With high-throughput Illumina Solexa sequencing technology, we have generated 59,859 high quality sequences from 12,918,391 paired-end reads. Using BLASTx/BLASTn, 12,227 contigs have blast hits in the Swissprot, NR protein database and NT nucleotide database with E-value cutoff 1e(-5. The comparison between the Octopus vulgaris central nervous system (CNS library and the Aplysia californica/Lymnaea stagnalis CNS ESTs library yielded 5.93%/13.45% of O. vulgaris sequences with significant matches (1e(-5 using BLASTn/tBLASTx. Meanwhile the hit percentage of the recently published Schistocerca gregaria, Tilapia or Hirudo medicinalis CNS library to the O. vulgaris CNS library is 21.03%-46.19%. We constructed the Phylogenetic tree using two genes related to CNS function, Synaptotagmin-7 and Synaptophysin. Lastly, we demonstrated that O. vulgaris may have a vertebrate-like Blood-Brain Barrier based on bioinformatic analysis. CONCLUSION: This study provides a mass of molecular information that will contribute to further molecular biology research on O. vulgaris. In our presentation of the first CNS transcriptome analysis of O. vulgaris, we hope to accelerate the study of functional molecular neurobiology and comparative evolutionary biology.

  15. СOMPARATIVE MORPHOLOGICAL AND ANATOMICAL INVESTIGATION OF THE VEGETATIVE ORGANS OF FILIPENDULA VULGARIS MOENCH. AND FILIPENDULA ULMARIA (L.) MAXIM. FROM THE NORTHERN CAUCASUS REGION

    OpenAIRE

    F. K. Serebryanaya; I. V. Geonya; K. M. Alieva

    2016-01-01

    Introduction. Study for raw sources of a phytogenesis to obtain biological active substances with expressed pharmacological activity, attracts undoubted interest for the development of modern pharmaceutics.Objective. To carry out a comparative morphological and anatomical research of two types of Filipendula species: Filipendula vulgaris Moench. and Filipendula ulmaria (L.) Maxim., which grows in the North Caucasus.Methods. Samples of fresh-gathered plants were gathered (June, 2014-2015) in v...

  16. Pemphigus vulgaris in a pregnant woman and her neonate

    Science.gov (United States)

    Ibrahim, Sameera Begum Kader; BM, Yashodhara; Umakanth, Shashikiran; Kanagasabai, Sachchithanatham

    2012-01-01

    A 23-year-old pregnant woman in her second trimester of pregnancy presented with blisters on the face, abdomen and the leg. Based on the clinical presentation and skin biopsy (histopathology and direct immunofluorescence) the diagnosis of pemphigus vulgaris was established. The child born to this patient also had similar skin lesions. The lesions in the mother and the child improved after treatment. The authors report a rare case of pemphigus vulgaris in a pregnant lady and neonatal pemphigus in her child, both of whom were treated successfully. PMID:22744241

  17. [Mutations in the gene encoding filaggrin cause ichthyosis vulgaris].

    Science.gov (United States)

    Prasad, Sumangali Chandra; Rasmussen, Kirsten; Bygum, Anette

    2011-02-14

    Ichthyosis vulgaris is a common genetic skin disorder with an estimated prevalence of 1:250 caused by mutations in the gene encoding filaggrin. This disorder manifests itself within the first year of life and is clinically characterized by dry, scaly skin, keratosis pilaris, palmar hyperlinearity and atopic manifestations. Patients with a severe phenotype are homozygous or compound heterozygous for the mutations, whereas heterozygous patients show mild disease, suggesting semidominant inheritance with incomplete penetrance. We present a patient with classic severe ichthyosis vulgaris, atopic eczema and two loss-of-function mutations.

  18. Coincident systemic lupus erythematosus and psoriasis vulgaris: a case report.

    Science.gov (United States)

    Wang, Y; Da, G; Yu, Y; Han, J; Li, H

    2015-12-01

    Psoriasis vulgaris is an autoimmune chronic inflammatory skin disease, but its association with other typical autoimmune disease such as systemic lupus erythematosus has only occasionally been reported. We presented a 25-year-old female who developed systemic lupus erythematosus associated with psoriasis vulgaris. Her conditions were in good control after she got administration of prednisolone (5 mg/day) and Tripterygium Wilfordii Hook (20 mg/day). It is necessary to integrate past history and physical examination to diagnose coincident SLE and psoriasis, and combined treatment with prednisolone and Tripterygium Wilfordii Hook proves effective.

  19. The dual nature of Interleukin-10 in pemphigus vulgaris

    Science.gov (United States)

    Cho, Michael Jeffrey; Ellebrecht, Christoph T.; Payne, Aimee S.

    2014-01-01

    The immunomodulatory cytokine interleukin-10 (IL-10) plays beneficial but also potentially detrimental roles in inflammation, infection, and autoimmunity. Recent studies suggest a regulatory role for IL-10-expressing B cells in the autoimmune blistering disease pemphigus vulgaris. Here we review the studies on IL-10 in pemphigus vulgaris and discuss the potential pathophysiological significance of these findings in comparison to prior studies of IL-10 in other human conditions. A better understanding of the complex roles of IL-10 in immune regulation may improve our understanding of pemphigus pathogenesis and treatment. PMID:25464924

  20. Novel protocol for lutein extraction from microalga Chlorella vulgaris

    DEFF Research Database (Denmark)

    D'Este, Martina; De Francisci, Davide; Angelidaki, Irini

    2017-01-01

    Lutein is a pigment generally extracted from marigold flowers. However, lutein is also found in considerable amounts in microalgae. In this study a novel method was developed to improve the extraction efficiency of lutein from microalga C. vulgaris. Differently from conventional methods, ethanol...... was used instead of water in the saponification step, which was conducted simultaneously to the solvent extraction, performed using dichloromethane. The amount of lutein extracted from C. vulgaris dried biomass increased more than threefold, from 0.20 ± 0.00 mgLutein/gDM to 0.69 ± 0.08 mgLutein/gDM. Lutein...

  1. Acne Vulgaris and Acne Rosacea: An Update in Etiopathogenesis

    Directory of Open Access Journals (Sweden)

    Ozlem Ekiz

    2014-04-01

    Full Text Available Acne vulgaris is a chronic inflammatory disease of the pilosebaceous unit, characterized by comedones, papules, pustules, nodules, and scars rarely. The major pathogenic factors are abnormal follicular differentiation and increased ductal cornification, abnormal activity of sebaceous glands, microbial colonization of pilosebaceous units by Propionibacterium acnes and inflammation. Rosacea is a common, chronic inflammatory relapsing skin disorder of the central area of the face characterized by transient or persistent erythema, telangiectasia, papules and pustules. Although several hypotheses have been suggested for the etiopathogenesis of rosacea, the exact etiology is still unknown. In this review, we tried to summarize up-to-date information about etiopathogenesis of acne vulgaris and rosocea.

  2. The dual nature of interleukin-10 in pemphigus vulgaris.

    Science.gov (United States)

    Cho, Michael Jeffrey; Ellebrecht, Christoph T; Payne, Aimee S

    2015-06-01

    The immunomodulatory cytokine interleukin-10 (IL-10) plays beneficial but also potentially detrimental roles in inflammation, infection, and autoimmunity. Recent studies suggest a regulatory role for IL-10-expressing B cells in the autoimmune blistering disease pemphigus vulgaris. Here we review the studies on IL-10 in pemphigus vulgaris and discuss the potential pathophysiological significance of these findings in comparison to prior studies of IL-10 in other human conditions. A better understanding of the complex roles of IL-10 in immune regulation may improve our understanding of pemphigus pathogenesis and treatment.

  3. 25 CFR 167.6 - Carrying capacities.

    Science.gov (United States)

    2010-04-01

    ... 25 Indians 1 2010-04-01 2010-04-01 false Carrying capacities. 167.6 Section 167.6 Indians BUREAU... Carrying capacities. (a) The Commissioner of Indian Affairs on June 26, 1943, promulgated the authorized carrying capacity for each land management district of the Navajo Reservation. (b) Recommended...

  4. 7 CFR 1437.402 - Carrying capacity.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 10 2010-01-01 2010-01-01 false Carrying capacity. 1437.402 Section 1437.402... Determining Coverage of Forage Intended for Animal Consumption § 1437.402 Carrying capacity. (a) CCC will establish a carrying capacity for all grazed forage present in the county for purposes of administering...

  5. A FEMINIST READING OF SISTER CARRIE

    Institute of Scientific and Technical Information of China (English)

    高陈科

    2011-01-01

    In the history of American literature, Sister Carrie has always been a controversial character. The critics regard Carrie either as a "fallen woman" or as a "new women". This thesis aims to offer a feminist reading of the image of Sister Carrie in the con

  6. Local Dynamic Stability Associated with Load Carrying

    Directory of Open Access Journals (Sweden)

    Jian Liu

    2013-03-01

    Conclusion: Current study confirmed the sensitivity of local dynamic stability measure in load carrying situation. It was concluded that load carrying tasks were associated with declined local dynamic stability, which may result in increased risk of fall accident. This finding has implications in preventing fall accidents associated with occupational load carrying.

  7. Pemphigus vulgaris. A presentation of 14 cases and review of the literature.

    Science.gov (United States)

    Camacho-Alonso, Fabio; López-Jornet, Pía; Bermejo-Fenoll, Ambrosio

    2005-01-01

    Pemphigus vulgaris (PV) is a chronic vesicular-ampullar mucocutaneous disease that almost always produces oral manifestations. The fact that blisters on the oral mucosa are sometimes the first manifestation of the disease implies that dental professionals must be sufficiently familiarized with the clinical manifestations of PV to ensure early diagnosis and treatment. We present a series of 14 patients with clinically and histologically diagnosed PV seen in the Teaching Unit of Oral Medicine of the University of Murcia (Spain) between 1981 and 2001. A thorough evaluation was made, recording patient age and sex, the location and extent of the lesions, and the signs and symptoms of the disease. Complementary studies were also carried out, with the evaluation of hematological parameters (including blood chemistry), the histology and immunohistochemical characteristics (direct immunofluorescence in 2 cases). Treatment comprised topical corticoids, in 12 cases combined with systemic corticoids, and associated to intralesional corticotherapy in one patient. A good response to treatment was observed in all cases.

  8. Bioremediation of the textile waste effluent by Chlorella vulgaris

    Directory of Open Access Journals (Sweden)

    Hala Yassin El-Kassas

    2014-01-01

    Full Text Available The microalgae biomass production from textile waste effluent is a possible solution for the environmental impact generated by the effluent discharge into water sources. The potential application of Chlorella vulgaris for bioremediation of textile waste effluent (WE was investigated using 22 Central Composite Design (CCD. This work addresses the adaptation of the microalgae C. vulgaris in textile waste effluent (WE and the study of the best dilution of the WE for maximum biomass production and for the removal of colour and Chemical Oxygen Demand (COD by this microalga. The cultivation of C. vulgaris, presented maximum cellular concentrations Cmax and maximum specific growth rates μmax in the wastewater concentration of 5.0% and 17.5%, respectively. The highest colour and COD removals occurred with 17.5% of textile waste effluent. The results of C. vulgaris culture in the textile waste effluent demonstrated the possibility of using this microalga for the colour and COD removal and for biomass production. There was a significant negative relationship between textile waste effluent concentration and Cmax at 0.05 level of significance. However, sodium bicarbonate concentration did not significantly influence the responses of Cmax and the removal of colour and COD.

  9. Separation of Chlorella vulgaris from liquid phase using bioflocculants

    Directory of Open Access Journals (Sweden)

    Gizem Günay

    2014-12-01

    results showed that C. vulgaris was partially separated from the liquid phase. However, the experiments will continue for the purpose of increasing the flocculating activity. Getting successfully experimental results with kaolin showed that bioflocculant has a potential use in wastewater treatment. For this reason, it also is thought to analyze the effect of bioflocculant on the wastewater treatment with further studies.[¤

  10. Acne vulgaris: A review of causes and treatment options.

    Science.gov (United States)

    Well, Danielle

    2013-10-10

    Acne vulgaris is a disorder of the sebaceous follicle. The cause is multifactorial, and both adolescents and adults can be affected. Acne is associated with a significant financial burden and considerable psychological distress. Treatment options are reviewed, including over-the-counter medications, prescription medications, and in-office procedures.

  11. [Molecular genetic investigation of sugar beet (Beta vulgaris L.)].

    Science.gov (United States)

    Butorina, A K; Kornienko, A V

    2011-10-01

    Molecular genetic studies of sugar beet (Beta vulgaris L.) are reviewed as a basis for the development of genomics of this species. The methods used to study structural and functional genomics are considered. The results and their application to increase the efficiency of sugar beet breeding are discussed.

  12. Dapsone 7.5% Gel: A Review in Acne Vulgaris.

    Science.gov (United States)

    Al-Salama, Zaina T; Deeks, Emma D

    2017-02-01

    Dapsone 7.5% gel (Aczone(®)) is indicated for the once-daily topical treatment of acne vulgaris in patients aged ≥12 years. Dapsone is a sulfone antibacterial with anti-inflammatory actions, which are thought to be largely responsible for its efficacy in treating acne vulgaris. In two phase III trials of 12 weeks' duration in patients aged ≥12 years with moderate acne vulgaris, once-daily dapsone 7.5% gel reduced acne severity (as per the Global Acne Assessment Score) and lesion counts versus vehicle. The benefits of dapsone 7.5% gel over vehicle were seen as early as week 2 for inflammatory lesion counts, and from week 4 or 8 for other outcomes. Dapsone 7.5% gel was well tolerated, with a low incidence of treatment-related adverse events, with the majority of adverse events being administration-site related and mild or moderate in severity. Thus, dapsone 7.5% gel is an effective and well tolerated option for the topical treatment of acne vulgaris in patients aged ≥12 years, with the convenience of once-daily application.

  13. Gallium-67-citrate uptake in a case of acne vulgaris

    Energy Technology Data Exchange (ETDEWEB)

    Kipper, M.S.; Taylor, A.; Ashburn, W.L.

    1981-09-01

    A case of increased Ga-67 uptake in a patient with active acne vulgaris is reported. The scan was requested in a search for metastatic testicular carcinoma or bleomycin pulmonary toxicity. Careful clinical evaluation including physical examination was necessary in order to avoid an erroneous scan interpretation.

  14. An update on the management of acne vulgaris

    Directory of Open Access Journals (Sweden)

    Jonette Keri

    2009-06-01

    Full Text Available Jonette Keri1,2, Michael Shiman11Department of Dermatology and Cutaneous Surgery, University of Miami Miller School of Medicine, Miami, FL, USA; 2Dermatology Service, Miami VA Hospital, FL, USAAbstract: Acne vulgaris is a common skin disorder that can affect individuals from childhood to adulthood, most often occurring in the teenage years. Acne can have a significant physical, emotional, and social impact on an individual. Many different treatment options are available for the treatment of acne vulgaris. Commonly used topical treatments include benzoyl peroxide, antibiotics, sulfur and sodium sulfacetamide, azelaic acid, and retinoids. Systemic treatment is frequently used and includes the use of systemic antibiotics, oral contraceptives, antiandrogens, and retinoids. Other treatment modalities exist such as the use of superficial chemical peels as well as using laser and light devices for the treatment of acne. With the multitude of treatment options and the rapidly expanding newer technologies available to clinicians, it is important to review and be aware of the current literature and studies regarding the treatment of acne vulgaris.Keywords: acne vulgaris, treatment, benzoyl peroxide, antibiotics, retinoids, lasers

  15. Vegetative propagation of Syringa vulgaris L. in vitro.

    NARCIS (Netherlands)

    Pierik, R.L.M.; Steegmans, H.H.M.; Elias, A.A.; Stiekema, O.T.J.; Velde, van der A.J.

    1988-01-01

    Excised shoot tips from adult Syringa vulgaris L. plants were rejuvenated by repeated subculturing in vitro. The number of subcultures required to rejuvenate the shoots was strongly dependent on the age and genotype of the plant material. Three rootstocks (K8, A2 and A3) and 5 cultivars (Mademoisell

  16. Evaluation of common bean (Phaseolus vulgaris) response to charcoal rot

    Science.gov (United States)

    Charcoal rot in common beans (Phaseolus vulgaris L.), caused by Macrophomina phaseolina (Tassi) Gold. (Mph), is an endemic disease in the prevailing hot and dry conditions in southern Puerto Rico. This study evaluated the 120 bean genotypes that compose the BASE 120 panel under screenhouse conditio...

  17. Pemphigus Vulgaris Activity Score and Assessment of Convergent Validity

    Directory of Open Access Journals (Sweden)

    Cheyda Chams-Davatchi

    2013-04-01

    Full Text Available Pemphigus is a rare autoimmune blistering disease with different phenotypes. The evaluation of therapeutic interventions requires a reliable, valid and feasible to use measurement. However, there is no gold standard to measure the disease activity in clinical trials. In this study we aimed to introduce the pemphigus vulgaris activity score (PVAS measurement and to assess the convergent validity with the experts’ opinion of disease activity. In PVAS scoring, the distribution of pemphigus vulgaris antigen expression in different anatomical regions is taking in to account with special consideration of the healing process. PVAS is a 0-18 scale, based on the extent of mucocutaneous involvement, type of lesion and the presence of Nikolsky’s sign. The sum of the scores of total number of lesions, number of different anatomic regions involvement and Nikolsky’s sign is weighted by the type of lesion. In the present study, PVAS was assessed in 50 patients diagnosed with pemphigus vulgaris by one dermatologist. Independently, five blinded experts scored all the patients through physician’s global assessment (PGA. The convergent validity with experts’ opinion was assessed. The Spearman coefficient of correlation showed the acceptable value of 0.751 (95%CI: 0.534- 0.876. PVAS is a valid, objective and simple-to-use scoring measurement. It showed a good correlation with PGA of pemphigus disease activity in Iranian patients with pemphigus vulgaris

  18. Variation in Breeding Systems in Hypericum Perforatum and Prunella Vulgaris

    Science.gov (United States)

    The effective conservation of new crop germplasm and its efficient use in new-crop development both rely on a clear understanding of the crop's reproductive biology. Hypericum perforatum (St. John's wort) and Prunella vulgaris (Common selfheal) are two medicinal plant species with potential for crop...

  19. Pemphigus vulgaris activity score and assessment of convergent validity.

    Science.gov (United States)

    Chams-Davatchi, Cheyda; Rahbar, Ziba; Daneshpazhooh, Maryam; Mortazavizadeh, Seyed Mohammad Ali; Akhyani, Maryam; Esmaili, Nafiseh; Balighi, Kamran

    2013-05-07

    Pemphigus is a rare autoimmune blistering disease with different phenotypes. The evaluation of therapeutic interventions requires a reliable, valid and feasible to use measurement. However, there is no gold standard to measure the disease activity in clinical trials. In this study we aimed to introduce the pemphigus vulgaris activity score (PVAS) measurement and to assess the convergent validity with the experts' opinion of disease activity. In PVAS scoring, the distribution of pemphigus vulgaris antigen expression in different anatomical regions is taking in to account with special consideration of the healing process. PVAS is a 0-18 scale, based on the extent of mucocutaneous involvement, type of lesion and the presence of Nikolsky's sign. The sum of the scores of total number of lesions, number of different anatomic regions involvement and Nikolsky's sign is weighted by the type of lesion. In the present study, PVAS was assessed in 50 patients diagnosed with pemphigus vulgaris by one dermatologist. Independently, five blinded experts scored all the patients through physician's global assessment (PGA). The convergent validity with experts' opinion was assessed. The Spearman coefficient of correlation showed the acceptable value of 0.751 (95%CI: 0.534- 0.876). PVAS is a valid, objective and simple-to-use scoring measurement. It showed a good correlation with PGA of pemphigus disease activity in Iranian patients with pemphigus vulgaris.

  20. Anaphylaxis to pine nut: cross-reactivity to Artemisia vulgaris?

    Science.gov (United States)

    Rodrigues-Alves, R; Pregal, A; Pereira-Santos, M C; Branco-Ferreira, M; Lundberg, M; Oman, H; Pereira-Barbosa, M

    2008-01-01

    The use of pine nuts, the seeds of Pinus pinea, is on the increasing in the modern Mediterranean diet. Little more than 20 cases of allergy to this tree nut have been published, and cross-reactivity with pine pollen, peanut and almond has already been reported. We describe the case of a young boy with several episodes of anaphylaxis after pine nut ingestion. Specific IgE to pine nut and Artemisia vulgaris was demonstrated by skin prick tests and in vitro determination of specific IgE, although no IgE to pine pollen or other nuts was detected. Immunoblotting of Artemisia vulgaris and pine nut revealed two matching diffuse bands, just below 14 kDa and 30 kDa. The ImmunoCAP inhibition assays showed complete inhibition of pine nut specific IgE after serum incubation with Artemisia vulgaris extract. As far as we know, this is the first reported case of documented cross-reactivity between pine nut and Artemisia vulgaris.

  1. The epidemiology of acne vulgaris in late adolescence

    Directory of Open Access Journals (Sweden)

    Lynn DD

    2016-01-01

    Full Text Available Darren D Lynn,1 Tamara Umari,1 Cory A Dunnick,2,3 Robert P Dellavalle2–4 1Department of Dermatology, University of Colorado School of Medicine, 2Department of Dermatology, University of Colorado Anschutz Medical Campus, Aurora, 3Dermatology Service, US Department of Veterans Affairs, Eastern Colorado Health Care System, Denver, 4Department of Epidemiology, Colorado School of Public Health, University of Colorado Anschutz Medical Campus, Aurora, CO, USA Importance: Acne vulgaris is the most common skin condition affecting late adolescents across the globe. Although prior studies have evaluated epidemiologic patterns of acne vulgaris in various ethnicities and regions, adequate understanding of the worldwide burden of the disease associated with patients in their late adolescence (15–19-year olds remains lacking. Objective: To assess the global burden of the disease associated with acne vulgaris for late adolescents (15–19-year olds and provide an overview of the epidemiology, pathophysiology, and treatment options for acne in this population. Design: Database summary study. Setting: Global Burden of Disease Study 2010 database. Participants: Global Burden of Disease regions comprised countries with prevalence of acne vulgaris between the ages of 15 and 19 years. Main outcomes and measures: Geographic region-level disability-adjusted life year rates (per 100,000 persons associated with acne vulgaris in years 1990 through 2010. Median percentage change in disability-adjusted life year rates was estimated for each region across the specified study period. Conclusion and relevance: Acne vulgaris-associated disease burden exhibits global distribution and has continued to grow in prevalence over time within this population. This continued growth suggests an unmet dermatologic need worldwide for this disorder and potential opportunities for improved access and delivery of dermatologic care. Our analysis of the literature reveals numerous

  2. EVALUACIÓN DEL EFECTO DEL HIDROCARBURO FENANTRENO SOBRE EL CRECIMIENTO DE Chlorella vulgaris (CHLORELLACEAE Effect of the Hydrocarbon Phenanthrene on Chlorella vulgaris (Chlorellaceae Growth

    Directory of Open Access Journals (Sweden)

    ANGÉLICA OTEROPATERNINA

    2013-04-01

    experiment. The assays were carried out in glass bottles of 0,4 l using the complex NPK (REMITAL® m - 17-6-18 at 1 g/l as an organic fertilizing. The results showed that phenanthrene inhibited progressively the alga growth being the lowest cellular growth observed in the medium with the highest phenanthrene concentration, reaching an inhibition percentage of 59 %. In the other treatments, the daily growth rate was relatively constant. The chlorophyll a concentration evaluated by spectrophotometry was not affected by the phenanthrene concentration. In conclusion, the growth of the alga C. vulgaris was affected negatively by the exposure to nominal concentrations of the polycyclic aromatic hydrocarbon phenanthrene higher than 1 µg/l.

  3. Identification of Small RNAs in Desulfovibrio vulgaris Hildenborough

    Energy Technology Data Exchange (ETDEWEB)

    Burns, Andrew; Joachimiak, Marcin; Deutschbauer, Adam; Arkin, Adam; Bender, Kelly

    2010-05-17

    Desulfovibrio vulgaris is an anaerobic sulfate-reducing bacterium capable of facilitating the removal of toxic metals such as uranium from contaminated sites via reduction. As such, it is essential to understand the intricate regulatory cascades involved in how D. vulgaris and its relatives respond to stressors in such sites. One approach is the identification and analysis of small non-coding RNAs (sRNAs); molecules ranging in size from 20-200 nucleotides that predominantly affect gene regulation by binding to complementary mRNA in an anti-sense fashion and therefore provide an immediate regulatory response. To identify sRNAs in D. vulgaris, a bacterium that does not possess an annotated hfq gene, RNA was pooled from stationary and exponential phases, nitrate exposure, and biofilm conditions. The subsequent RNA was size fractionated, modified, and converted to cDNA for high throughput transcriptomic deep sequencing. A computational approach to identify sRNAs via the alignment of seven separate Desulfovibrio genomes was also performed. From the deep sequencing analysis, 2,296 reads between 20 and 250 nt were identified with expression above genome background. Analysis of those reads limited the number of candidates to ~;;87 intergenic, while ~;;140 appeared to be antisense to annotated open reading frames (ORFs). Further BLAST analysis of the intergenic candidates and other Desulfovibrio genomes indicated that eight candidates were likely portions of ORFs not previously annotated in the D. vulgaris genome. Comparison of the intergenic and antisense data sets to the bioinformatical predicted candidates, resulted in ~;;54 common candidates. Current approaches using Northern analysis and qRT-PCR are being used toverify expression of the candidates and to further develop the role these sRNAs play in D. vulgaris regulation.

  4. Sister Carrie:A Material Pursuer

    Institute of Scientific and Technical Information of China (English)

    马春花

    2015-01-01

    Sister Carrie dramatized by Dreiser is totally a material pursuer. She is selfish and accumulates money in a crazy way. What she does inevitably centers on materials. Living with Drouet and later Hurstwood, Carrie gets what she wants and enjoys the luxurious life in an easy way. However, with the satisfaction of some of her desires, Carrie ’s desires grow and expand. With enough food and clothes, she needs luxury. Hurstwood’s failure in business leads Carrie to the stage and finally she makes a suc⁃cess and becomes a famous actress in Broadway. She gets more money, but her desires grow even higher. Nothing can satisfy her. In this essay, the author tries to analyze Carrie according to Freud’s and Guo Weilu’s theories and prove that Carrie is totally a material pursuer.

  5. ICG laser therapy of acne vulgaris

    Science.gov (United States)

    Tuchin, Valery V.; Altshuler, Gregory B.; Genina, Elina A.; Bashkatov, Alexey N.; Simonenko, Georgy V.; Odoevskaya, Olga D.; Yaroslavsky, Ilya V.

    2004-07-01

    The near-infrared (NIR) laser radiation due to its high penetration depth is widely used in phototherapy. In application to skin appendages a high selectivity of laser treatment is needed to prevent light action on surrounding tissues. Indocyanine Green (ICG) dye may provide a high selectivity of treatment due to effective ICG uploading by a target and its narrow band of considerable absorption just at the wavelength of the NIR diode laser. The goal of this study is to demonstrate the efficacy of the NIR diode laser phototherapy in combination with topical application of ICG suggested for soft and thermal treatment of acne vulgaris. 28 volunteers with facile or back-located acne were enrolled. Skin sites of subjects were stained by ICG and irradiated by NIR laser-diode light (803 or 809 nm). Untreated, only stained and only light irradiated skin areas served as controls. For soft acne treatment, the low-intensity (803 nm, 10 - 50 mW/cm2, 5-10 min) or the medium-intensity (809 nm, 150 - 190 mW/cm2, 15 min) protocols were used. The single and multiple (up to 8-9) treatments were provided. The individual acne lesions were photothermally treated at 18 W/cm2 (803 nm, 0.5 sec) without skin surface cooling or at 200 W/cm2 (809 nm, 0.5 sec) with cooling. The results of the observations during 1-2 months after the completion of the treatment have shown that only in the case of the multiple-wise treatment a combined action of ICG and NIR irradiation reduces inflammation and improves skin state during a month without any side effects. At high power densities (up to 200 W/cm2) ICG stained acne inflammatory elements were destructed for light exposures of 0.5 sec. Based on the concept that hair follicle, especially sebaceous gland, can be intensively and selectively stained by ICG due to dye diffusion through pilosebaceous canal and its fast uptake by living microorganisms, by vital keratinocytes of epithelium of the canal and sebaceous duct, and by rapidly proliferating

  6. Pemphigus vulgaris autoantibody profiling by proteomic technique.

    Directory of Open Access Journals (Sweden)

    Mina Kalantari-Dehaghi

    Full Text Available Pemphigus vulgaris (PV is a mucocutaneous blistering disease characterized by IgG autoantibodies against the stratified squamous epithelium. Current understanding of PV pathophysiology does not explain the mechanism of acantholysis in patients lacking desmoglein antibodies, which justifies a search for novel targets of pemphigus autoimmunity. We tested 264 pemphigus and 138 normal control sera on the multiplexed protein array platform containing 701 human genes encompassing many known keratinocyte cell-surface molecules and members of protein families targeted by organ-non-specific PV antibodies. The top 10 antigens recognized by the majority of test patients' sera were proteins encoded by the DSC1, DSC3, ATP2C1, PKP3, CHRM3, COL21A1, ANXA8L1, CD88 and CHRNE genes. The most common combinations of target antigens included at least one of the adhesion molecules DSC1, DSC3 or PKP3 and/or the acetylcholine receptor CHRM3 or CHRNE with or without the MHC class II antigen DRA. To identify the PV antibodies most specific to the disease process, we sorted the data based on the ratio of patient to control frequencies of antigen recognition. The frequency of antigen recognition by patients that exceeded that of control by 10 and more times were the molecules encoded by the CD33, GP1BA, CHRND, SLC36A4, CD1B, CD32, CDH8, CDH9, PMP22 and HLA-E genes as well as mitochondrial proteins encoded by the NDUFS1, CYB5B, SOD2, PDHA1 and FH genes. The highest specificity to PV showed combinations of autoantibodies to the calcium pump encoded by ATP2C1 with C5a receptor plus DSC1 or DSC3 or HLA-DRA. The results identified new targets of pemphigus autoimmunity. Novel autoantibody signatures may help explain individual variations in disease severity and treatment response, and serve as sensitive and specific biomarkers for new diagnostic assays in PV patients.

  7. Measuring Social Carrying Capacity: An Exploratory Study

    OpenAIRE

    López-Bonilla, Jesús Manuel; López-Bonilla, Luis Miguel

    2007-01-01

    The tourist carrying capacity commands a growing interest given that it is closely linked with sustainable tourist development. The justification of the utility of this concept is given by means of a simple and efficient methodological proposal, by analysing the social carrying capacity. To this end, an empirical application is carried out in the Western Andalusia. In some of the cases analysed, the satisfaction of the tourist is found to decline when the levels of the tourist use are higher ...

  8. ENVIRONMENTAL CARRYING CAPACITY BASED ON SPATIAL PLANNING

    OpenAIRE

    Luthfi Muta'ali

    2013-01-01

    The aims of this research were to examine environmental carrying capacity analyzed based on aspects of spatial planning and eco-region. The result showed that Kulonprogo Regency has low value of environmental carrying capacity and can only support as much as 79.81% of its total population. Analysis of variance showed significant difference of environmental carrying capacity of protected and cultivated area. The main factor among 12 variables determining the degree of environmen...

  9. Bivalve carrying capacity in coastal ecosystems

    NARCIS (Netherlands)

    Dame, R.F.; Prins, T.C.

    1998-01-01

    carrying capacity of suspension feeding bivalves in 11 coastal and estuarine ecosystems is examined. Bivalve carrying capacity is defined in terms of water mass residence time, primary production time and bivalve clearance time. Turnover times for the 11 ecosystems are compared both two and three di

  10. Comments on the image of Sister Carrie

    Institute of Scientific and Technical Information of China (English)

    张楠

    2016-01-01

    Thedore Oreiser was one of America's greatest writers and one of his famous masterpieces is Sister Carrie. the heroin of the novel was a country girl who struggled for success and finally became a movie star. Analysis on the image of Carrie is of practical significance to the country girls swarming into the city nowdays in our country.

  11. Carrie Chapman Catt and Woman Suffrage.

    Science.gov (United States)

    Hardesty, Carolyn, Ed.

    1989-01-01

    Most of the material for this issue of the "Goldfinch," which explores the life of Carrie Chapman Catt, came from the archives of the State Historical Society of Iowa. Carrie Chapman Catt (1859-1947) was an Iowan who advocated woman suffrage and spent 26 years actively working for that cause. The issue contains a biography of Catt, and…

  12. Parallelization of Reversible Ripple-carry Adders

    DEFF Research Database (Denmark)

    Thomsen, Michael Kirkedal; Axelsen, Holger Bock

    2009-01-01

    The design of fast arithmetic logic circuits is an important research topic for reversible and quantum computing. A special challenge in this setting is the computation of standard arithmetical functions without the generation of \\emph{garbage}. Here, we present a novel parallelization scheme...... wherein $m$ parallel $k$-bit reversible ripple-carry adders are combined to form a reversible $mk$-bit \\emph{ripple-block carry adder} with logic depth $\\mathcal{O}(m+k)$ for a \\emph{minimal} logic depth $\\mathcal{O}(\\sqrt{mk})$, thus improving on the $mk$-bit ripple-carry adder logic depth $\\mathcal......{O}(m\\cdot k)$. The underlying mechanisms of the parallelization scheme are formally proven correct. We also show designs for garbage-less reversible comparison circuits. We compare the circuit costs of the resulting ripple-block carry adder with known optimized reversible ripple-carry adders in measures...

  13. The Concept of Carrying Capacity in Tourism

    Directory of Open Access Journals (Sweden)

    Josef Zelenka

    2014-05-01

    Full Text Available Carrying capacity is often pragmatically, theoretically as well as purely intuitively considered as a concept in the context of tourism sustainability. The carrying capacity application has the greatest potential in protected areas, in frequently visited cultural and natural attractions, and in relation to sustaining of the lifestyle of the local community and tourism destination potential in general. Despite its importance, partial applications, determination of basic theoretical principles, and specifying connection to the other theoretical concepts in tourism (particularly destination life cycle, LAC concept, visitors management, there still is a rightful opinion of some authors suggesting that there is no consistent theory of tourism carrying capacity. This theory would be the base for sophisticated practical carrying capacity applications. This paper is therefore focused on introduction of the theoretical concept of carrying capacity, which can be discussed and possibly further elaborated.

  14. Sister Carrie, an Adherent of Desires

    Institute of Scientific and Technical Information of China (English)

    裴水妹

    2007-01-01

    Sister Carrie is one of the most controversial characters in American literature.Thought as a "fallen woman" firstly,she was defined as a "new woman" by some critics later. However, by digging into the motivaton behind the whole process of Carrie's "success", the relationship between Carrie and her creator (the author), the social conditions of then American, it can be found that Carrie has never been free-standing on her thought and she has never found her real-sdf even after becoming a famous actress. In a society dominated by mass consumerism Carrie is only an adherent of her own desires. She also is a representative of all those country girls flooded into cities, a symbol and a sacrifice of the urbanization of America in a time countryside was overcome by cities.

  15. The optimal hyperspectral quantitative models for chlorophyll-a of chlorella vulgaris

    Science.gov (United States)

    Cheng, Qian; Wu, Xiuju

    2009-09-01

    Chlorophyll-a of Chlorella vulgaris had been related with spectrum. Based on hyperspectral measurement for Chlorella vulgaris, the hyperspectral characteristics of Chlorella vulgaris and their optimal hyperspectral quantitative models of chlorophyll-a (Chla) estimation were researched in situ experiment. The results showed that the optimal hyperspectral quantitative model of Chlorella vulgaris was Chla=180.5+1125787(R700)'+2.4 *109[(R700)']2 (P0Chlorella vulgaris, two reflectance crests were around 540 nm and 700 nm and their locations moved right while Chl-a concentration increased. The reflectance of Chlorella vulgaris decreases with Cha concentration increase in 540 nm, but on the contrary in 700nm.

  16. A preliminary screening study on the associated proteins in human psoriasis vulgaris by serum proteomics technologies

    Institute of Scientific and Technical Information of China (English)

    Zhankui Liu; Shengshun Tan; Chunshui Yu; Jinghua Fan; Zhuanli Bai; Junjie Li

    2007-01-01

    Objective:To investigate the optimum screening conditions of associated proteins in human psoriasis vulgaris by serum proteomics technique, and to screen the different expression proteins related with psoriasis vulgaris. Methods:Serum samples of peripheral blood were collected from newly diagnosed psoriasis vulgaris patients in the clinic, and 20 matched healthy persons.Serum albumin IgG was removed by filtering with ProteoExtract Albumin/IgG. After comparative proteomics analysis the different protein spots were identified using 2-DE and MS. Results :Electrophoresis figures with high resolution and reproducibility were obtained. Three different expression proteins were found only in the serum from psoriasis vulgaris patients,while nine other different proteins expressing from healthy volunteers. Conclusion:The protein expression was different in the serum between the psoriasis vulgaris patients and healthy volunteers. It was hoped that we could find the biomarkers related to psoriasis vulgaris by using proteomics.

  17. Gun Carrying by High School Students in Boston, MA: Does Overestimation of Peer Gun Carrying Matter?

    Science.gov (United States)

    Hemenway, David; Vriniotis, Mary; Johnson, Renee M.; Miller, Matthew; Azrael, Deborah

    2011-01-01

    This paper investigates: (1) whether high school students overestimate gun carrying by their peers, and (2) whether those students who overestimate peer gun carrying are more likely to carry firearms. Data come from a randomly sampled survey conducted in 2008 of over 1700 high school students in Boston, MA. Over 5% of students reported carrying a…

  18. Gun Carrying by High School Students in Boston, MA: Does Overestimation of Peer Gun Carrying Matter?

    Science.gov (United States)

    Hemenway, David; Vriniotis, Mary; Johnson, Renee M.; Miller, Matthew; Azrael, Deborah

    2011-01-01

    This paper investigates: (1) whether high school students overestimate gun carrying by their peers, and (2) whether those students who overestimate peer gun carrying are more likely to carry firearms. Data come from a randomly sampled survey conducted in 2008 of over 1700 high school students in Boston, MA. Over 5% of students reported carrying a…

  19. Carry Select Adder Circuit with A Successively Incremented Carry Number Block

    OpenAIRE

    Deepak; Bal Krishan

    2014-01-01

    This paper reports a conditional carry select (CCS) adder circuit with a successively-incremented-carry-number block (SICNB) structure for low-voltage VLSI implementation. Owing to the successively-incremented-carry-number block (SICNB) structure, the new 16-bit SICNB CCS adder provides a 37% faster speed as compared to the conventional conditional Carry select adder based on the SPICE results

  20. Mutualistic growth of the sulfate-reducer Desulfovibrio vulgaris Hildenborough with different carbohydrates.

    Science.gov (United States)

    Santana, M M; Portillo, M C; Gonzalez, J M

    2012-01-01

    Desulfovibrio vulgaris Hildenborough genome presents a phosphotransferase system putatively involved in the transport of carbohydrates. However, utilization of sugars by this sulfate-reducing bacterium has never been reported. Herein, we have observed proliferation of D. vulgaris Hildenborough with some carbohydrates, in mutualism with Stenotrophomonas maltophilia, a non-fermentative, gram-negative gammaproteobacterium, or Microbacterium, a gram-positive actinobacterium. These results suggest the importance of feedback interactions between different heterotrophic bacterial species including the alternative for D. vulgaris of exploiting additional organic resources and novel habitats. Thus, D. vulgaris strongly participates in the mineralization of carbohydrates both in complex natural and artificial systems.

  1. Interaction between Chlorella vulgaris and bacteria:interference and resource competition

    Institute of Scientific and Technical Information of China (English)

    QU Liang; WANG Renjun; ZHAO Peng; CHEN Ruinan; ZHOU Wenli; TANG Liuqing; TANG Xuexi

    2014-01-01

    Research of interaction mechanism between Chlorella vulgaris and two bacterial strains (Z-QD08 and Z-QS01) were conducted under laboratory conditions. Growth rates of bacteria and C. vulgaris were tested under co-culture conditions to evaluate the effects of concentrations of C. vulgaris and bacteria on their interactions. To test whether the availability of inorganic nutrients, vitamins and trace metals affects the interactions between C. vulgaris and bacteria, experiments were performed with or without the culture medium filtrate of C. vulgaris or bacteria. The results showed that the growth of C. vulgaris was promot-ed at low concentrations of bacteria (5×106 cells/ml), and expressed a positive correlation with the bacteria density, whereas opposite trend was observed for treatments with high bacteria density (10×106 cells/ml and 20×106 cells/ml). The growth rate of bacteria decreased with the increasing concentrations of C. vul-garis. The growth of bacteria Z-QD08 was inhibited by C. vulgaris through interference competition, while the mechanism for interaction between bacteria Z-QS01 and C. vulgaris was resource competition. The influence of cell density on the interaction between microalgae and bacteria was also discussed. These ex-periments confirm some elements of published theory on interactions between heterotrophic bacteria and microalgae and suggest that heterotrophic bacteria play an important role in the development of blooms in natural waters.

  2. Mucosal-dominant pemphigus vulgaris in a captopril-taking woman with angioedema.

    Science.gov (United States)

    Gornowicz-Porowska, Justyna; Dmochowski, Marian; Pietkiewicz, Pawel; Bowszyc-Dmochowska, Monika

    2015-01-01

    We describe a 39-year-old woman with an apparent captopril-induced, contact mucosal-dominant pemphigus vulgaris and angioedema, who took captopril during a bout of arterial hypertension. This exposure suggests that captopril and pathophysiology of angioedema stimulated the development of pemphigus vulgaris, which was diagnosed using the novel, indirect immunofluorescence BIOCHIP mosaic, with the modification to detect serum IgG4 autoantibodies. We discuss the patient, who experienced a chain of events leading to the active stage of pemphigus vulgaris, and review concepts of pemphigus vulgaris inducible by drugs and pathological immunity.

  3. Changes in weed infestations on plantations of sugar beet (Beta vulgaris L. subsp. vulgaris cultivated on black soil near Wrocław in 1989–1995 and 2006–2012

    Directory of Open Access Journals (Sweden)

    Krzysztof Domaradzki

    2016-03-01

    Full Text Available Field studies were carried out in 1989–1995 and 2006–2012 on plantations of sugar beet (Beta vulgaris L. subsp. vulgaris. During this period, 542 phytosociological relevés were made using the Braun-Blanquet method. In total, 46 weed species were found. In 1989–1995, the occurrence of 36 segetal species was reported. The highest cover indices were determined for Chenopodium album and Amaranthus retroflexus. Galium aparine, Echinochloa crus-galli, and Elymus repens were the dominant species, as well. Analysis of the frequency of occurrence revealed one constant species (Chenopodium album, two frequent species (Amaranthus retroflexus and Galium aparine, and two medium-frequent species (Echinochloa crus-galli and Matricaria maritima ssp. inodora. In 2006–2012, the occurrence of 40 weed species on the sugar beet plantations was recorded. The plantations were clearly dominated by Chenopodium album, accompanied by Polygonum persicaria and Polygonum lapathifolium ssp. lapathifolium. Other dominant species comprised Setaria viridis, Galinsoga parviflora, Brassica napus ssp. napus, and Fallopia convolvulus. The Chenopodium album was a constant component of the sugar beet plantations. In turn, no frequent species were observed and six medium-frequent species were found (Setaria viridis, Galinsoga parviflora, Brassica napus ssp. napus, Echinochloa crus-galli, Amaranthus retroflexus, and Capsella bursa-pastoris. Noteworthy, the presence of previously unreported species, e.g., Abutilon theophrasti, Hyoscyamus niger, or Artemisia vulgaris, was revealed. These species are rare components in sugar beet crops. A reverse phenomenon, i.e., the disappearance of some species such as Euphorbia helioscopia, Malva neglecta, Rumex acetosella, Sinapis arvensis, or Sisymbrium officinale, was also observed.

  4. CO2 Biofixation and Growth Kinetics of Chlorella vulgaris and Nannochloropsis gaditana.

    Science.gov (United States)

    Adamczyk, Michał; Lasek, Janusz; Skawińska, Agnieszka

    2016-08-01

    CO2 biofixation was investigated using tubular bioreactors (15 and 1.5 l) either in the presence of green algae Chlorella vulgaris or Nannochloropsis gaditana. The cultivation was carried out in the following conditions: temperature of 25 °C, inlet-CO2 of 4 and 8 vol%, and artificial light enhancing photosynthesis. Higher biofixation were observed in 8 vol% CO2 concentration for both microalgae cultures than in 4 vol%. Characteristic process parameters such as productivity, CO2 fixation, and kinetic rate coefficient were determined and discussed. Simplified and advanced methods for determination of CO2 fixation were compared. In a simplified method, it is assumed that 1 kg of produced biomass equals 1.88 kg recycled CO2. Advance method is based on empirical results of the present study (formula with carbon content in biomass). It was observed that application of the simplified method can generate large errors, especially if the biomass contains a relatively low amount of carbon. N. gaditana is the recommended species for CO2 removal due to a high biofixation rate-more than 1.7 g/l/day. On day 10 of cultivation, the cell concentration was more than 1.7 × 10(7) cells/ml. In the case of C. vulgaris, the maximal biofixation rate and cell concentration did not exceed 1.4 g/l/day and 1.3 × 10(7) cells/ml, respectively.

  5. Response of Phaseolus vulgaris L. plants to low-let ionizing radiation: Growth and oxidative stress

    Science.gov (United States)

    Arena, C.; De Micco, V.; Aronne, G.; Pugliese, M.; Virzo De Santo, A.; De Maio, A.

    2013-10-01

    The scenarios for the long-term habitation of space platforms and planetary stations involve plants as fundamental part of Bioregenerative Life Support Systems (BLSS) to support the crew needs. Several constraints may limit plant growth in space: among them ionizing radiation is recognized to severely affect plant cell at morphological, physiological and biochemical level. In this work, plants of Phaseolus vulgaris L. were subjected to four different doses of X-rays (0.3, 10, 50 and 100 Gy) in order to assess the effects of ionizing radiation on this species and to analyze possible mechanisms carried out to overcome the radiation injuries. The effects of X-rays on plant growth were assessed by measuring stem elongation, number of internodes and leaf dry weight. The integrity of photosynthetic apparatus was evaluated by photosynthetic pigment composition and ribulose 1,5-bisphosphate carboxylase (Rubisco) activity, whereas changes in total antioxidant pool and glutathione S transferase activity (GST) were utilized as markers of oxidative stress. The distribution of phenolic compounds in leaf tissues as natural shielding against radiation was also determined. Irradiation of plants at 0.3 and 10 Gy did not determine differences in all considered parameters as compared to control. On the contrary, at 50 and 100 Gy a reduction of plant growth and a decrease in photosynthetic pigment content, as well as an increase in phenolic compounds and a decrease in total antioxidant content and GST activity were found. Only a slight reduction of Rubisco activity in leaves irradiated at 50 and 100 Gy was found. The overall results indicate P. vulgaris as a species with a good potential to face ionizing radiation and suggest its suitability for utilization in BLSSs.

  6. Effectivity of Beta vulgaris L. Extract with various Solvent Fractions to Aedes aegypti Larval Mortality

    Directory of Open Access Journals (Sweden)

    Mutiara Widawati

    2013-06-01

    Full Text Available Dengue vector control is mostly done by using plant-based insecticides. Insecticides from the vegetable and fruit extracts of the leaves of plants that contain compounds alkaloids, saponins, flavonoids, tannins, triterpenoids, and polyphenols can be used as an alternative to naturally control Ae. aegypti. The purpose of this study was to determine the effectiveness of the B. vulgaris L. extract larvacide against larvae of Ae. aegypti. The materials that been used was B. vulgaris L. fruit parts which was milled and dried to become a powder form. 800 g of dry powder was extracted by 70% methanol by percolation method with occasional stirring for 3 days. The extract was concentrated using an evaporator. 60 g remaining residue was dissolved in distilled water and re-extracted with diethyl ether, chloroform, and ethyl acetate. Each fraction extract was dried with anhydrous sodium sulfate and the solvent was distilled. The extract was tested qualitatively to determine the content of secondary metabolites. Larvacide test performed by dissolving each extracts in dimetilsulphoxide (DMSO at concentrations of 0.1, 0.5 and 1%. The larvae used was larval of Ae. aegypti age of seven days. Death larvae counted every day for seven days to determine the effect of the contact. Tests carried out at a temperature of 27±1°C by immersing 25 larvae at each concentration of the extract with 50 mL volume and three replications was performed. The data obtained were analyzed further with different test. The results showed that fruit extract contains flavonoids, alkoloid, sterols, triterpenes, saponins and tannins. Highest mortality happened which was 82.5% and the lowest mortality happened with a concentration of 0.1% diethyl ether extract fraction. The extracts that are dissolved in various solvent fractions have not been effective as a larvacide until the highest concentration which was 1%. Methanol and polar solvent extracts of the fruit has a larvacide potency a bit

  7. Effects of carrying methods and box handles on two-person team carrying capacity for females.

    Science.gov (United States)

    Wu, Swei-Pi; Chang, Shu-Yu

    2010-07-01

    This study used a psychophysical approach to examine the effects of carrying methods and the presence or absence of box handles on the maximum acceptable weight carried and resulting responses (heart rate and rating of perceived exertion) in a two-person carrying task. After training, 16 female subjects performed a two-person carrying task at knuckle height for an 8-h work period. Each subject performed 4 different carrying combinations two times. The independent variables were carrying methods (parallel and tandem walking) and box handles (with and without handles). For comparison with two-person carrying, the subjects also performed one-person carrying. The results showed that the maximum acceptable weight carried (MAWC), heart rate (HR), and rating of perceived exertion (RPE) were significantly affected by the presence of box handles. However, the subjects' MAWC, HR, and RPE values were not significantly influenced by the carrying methods. The test-retest reliability of the psychophysical approach was 0.945. The carrying efficiency of two-person carrying was 96.2% of the one-person carrying method. In general, the use of box with handles allows the subjects to carry a higher MAWC (with lower HR and RPE) compared to carrying boxes without handles.

  8. Gravitational waves carrying orbital angular momentum

    CERN Document Server

    Bialynicki-Birula, Iwo

    2015-01-01

    Spinorial formalism is used to map every electromagnetic wave into the gravitational wave (within the linearized gravity). In this way we can obtain the gravitational counterparts of Bessel, Laguerre-Gauss, and other light beams carrying orbital angular momentum.

  9. POPULATION DENSITIES AND THE RANGE-CARRYING ...

    African Journals Online (AJOL)

    CAPACITY FOR LARGE MAMMALS IN QUEEN ELIZABETH ... The highest known densities oflarge terrestrial mammals occur on the grasslands in ...... It is estimated that the proper average biomass carrying capacity for the several herbivore.

  10. Transgenic cassava lines carrying heterologous alternative oxidase ...

    African Journals Online (AJOL)

    To screen positive lines for gene function, leaf lobes from two transgenic lines with a line carrying an empty vector and the wild type were subjected to somatic embryogenesis (SE), a known oxidative ... African Journal of Biotechnology Vol.

  11. A Naturalistic Reading of Sister Carrie

    Institute of Scientific and Technical Information of China (English)

    谈月

    2016-01-01

    Sister Carrie is well known as the works in which naturalism attained maturity in America. Up until now, the relevant research on Dreiser and his Sister Carrie abroad and at home is primarily concerned with the frustration of American dream, the naturalistic thoughts and pessimism. The paper attempts to study it from naturalistic point of view and explain how environmental, hereditary factors and the idea of“survival of the fittest”influence Carrie’s fate.

  12. Thymus vulgaris essential oil: chemical composition and antimicrobial activity.

    Science.gov (United States)

    Borugă, O; Jianu, C; Mişcă, C; Goleţ, I; Gruia, A T; Horhat, F G

    2014-01-01

    The study was designed to determine the chemical composition and antimicrobial properties of the essential oil of Thymus vulgaris cultivated in Romania. The essential oil was isolated in a yield of 1.25% by steam distillation from the aerial part of the plant and subsequently analyzed by GC-MS. The major components were p-cymene (8.41%), γ-terpinene (30.90%) and thymol (47.59%). Its antimicrobial activity was evaluated on 7 common food-related bacteria and fungus by using the disk diffusion method. The results demonstrate that the Thymus vulgaris essential oil tested possesses strong antimicrobial properties, and may in the future represent a new source of natural antiseptics with applications in the pharmaceutical and food industry.

  13. Case of lupus vulgaris diagnosed 50 years after onset.

    Science.gov (United States)

    Uttawichai, Pattanawadee; Igarashi, Tsukasa; Kawana, Seiji

    2009-02-01

    Cutaneous tuberculosis is an infrequent form of extrapulmonary tuberculosis, but is a symptom that can lead to diagnosis of tuberculosis. We describe a case of lupus vulgaris in a 79-year-old woman who had a 50-year history of a slowly growing plaque on her right cheek. She visited many hospitals without resolution and the plaque gradually enlarged. Recently, she was misdiagnosed with eczema and prescribed topical steroids that had no effect, and she subsequently visited our outpatient clinic. A diagnosis of lupus vulgaris was made based on histopathology, culture and polymerase chain reaction, and isoniazid, rifampicin and ethambutol were administered as antituberculosis treatment. Although the incidence of cutaneous tuberculosis has decreased significantly in developed countries, knowledge and awareness of the disease are still of importance for proper diagnosis and treatment.

  14. Biodiesel production in crude oil contaminated environment using Chlorella vulgaris.

    Science.gov (United States)

    Xaaldi Kalhor, Aadel; Mohammadi Nassab, Adel Dabbagh; Abedi, Ehsan; Bahrami, Ahmad; Movafeghi, Ali

    2016-12-01

    Biodiesel is a valuable alternative to fossil fuels and many countries choose biodiesel as an unconventional energy source. A large number of investigations have been done on microalgae as a source of oil production. In recent years, wastewater pollutions have caused many ecological problems, and therefore, wastewater phycoremediation has attracted the international attention. This paper studied the cultivation of Chlorella vulgaris in a crude oil polluted environment for biodiesel production. Intended concentrations were 10 and 20gperliter (crude oil/water) at two times. The results showed that the growth of C. vulgaris was improved in wastewater and the maximum amount of dry mass and oil was produced at the highest concentration of crude oil (0.41g and 0.15g/l, respectively). In addition, dry mass and oil yield of the microalga were significantly enhanced by increasing the experiment duration.

  15. EFFECT OF CAPSAICIN ON SUBSTANCE P IN PSORIASIS VULGARIS

    Institute of Scientific and Technical Information of China (English)

    王万卷; 谭升顺

    2003-01-01

    Objective To the investigate the mechanism of capsaicin in psoriasis vulgaris. Methods Substance P(SP) in psoriatic lesions before and 6 weeks after the treatment with capsaicin was detedted by radioimmunoassary. Results After 3 weeks and 6 weeks treatment with capsaicin, SP in psoriatic lesions was decreased (P<0.05), while it in the self-control group was not decreased; Overall the efficient incidence in therapeutic group was 78.8% , while it in the control group was 36.8%. There was significant difference between them (χ2=16.30, P<0.001). Conclusion Capsaicin inhibits dermal inflammatory responses and proliferation of keratinocytes by decreasing the expression of SP in psoriasis vulgaris.

  16. Ulcerative lupus vulgaris of face: an uncommon presentation in India.

    Science.gov (United States)

    Padmavathy, L; Rao, L Lakshmana; Ethirajan, N; Krishnaswami, B

    2007-01-01

    Tuberculosis affects the population world wide, more among those living in developing countries. The incidence of tuberculosis registered an upward trend even in developed countries, with the advent of HIV infection. Cutaneous tuberculosis accounts for about 1% of cases of extra-pulmonary tuberculosis. Cutaneous tuberculosis presents with various lesions ranging from ulcerative to proliferative or hyperkeratotic lesions. The lesions may sometimes be associated with marked destruction of the tissues resulting in marked disfigurement, especially when it involves face as seen in cases of Lupus Vulgaris. A case of Lupus Vulgaris in a young woman with extensive ulceration of face which responded to ATT resulting in scarring of the face is reported for its rarity amongst Indian population as against western population.

  17. Annular lupus vulgaris: an unusual case undiagnosed for five years.

    Science.gov (United States)

    Gönül, Müzeyyen; Kiliç, Arzu; Külcü Cakmak, Seray; Gül, Ulker; Koçak, Oğuzhan; Demiriz, Murat

    2007-01-01

    Tuberculosis is still a serious problem in both developing and developed countries. It is often confused with various cutaneous disorders both clinically and histopathologically.A 46-year-old woman attended our clinic with progressive, asymptomatic, annular skin lesions on her right upper extremity for 5 years. She had received many different therapies for these lesions at other institutions previously but these medications were not effective and the lesions deteriorated. On dermatological examination, well-demarcated, irregular bordered, violaceous colored, elevated and crusted annular lesions on her right hand dorsum and forearm were observed. She was diagnosed as having lupus vulgaris clinically and histopathologically. Antituberculosis therapy was administered and regression of the lesions started in the second week of medication.We report a case of long-standing, undiagnosed and uncommon, annular form of lupus vulgaris. We want to stress that clinical and histopathological findings are still important for the diagnosis of cutaneous tuberculosis.

  18. Phaseolus vulgaris RbohB functions in lateral root development

    Science.gov (United States)

    Montiel, Jesús; Arthikala, Manoj-Kumar; Quinto, Carmen

    2013-01-01

    Respiratory burst oxidase homologs (RBOHs) catalyze the reduction of oxygen to generate superoxide anion, a kind of reactive oxygen species (ROS). The ROS produced by RBOHs play essential roles in diverse processes, such as root hair development, stomata closure and signaling mechanisms in response to abiotic stimuli and during plant-pathogen interactions. Recently, we found that PvRbohB silencing in transgenic Phaseolus vulgaris roots had a negative impact on lateral root density. In this work, we show that the downregulation of PvRbohB affects both the growth and ROS levels in recently emerged lateral roots. In addition, we found that the PvRbohB promoter was activated during lateral root primordium initiation in the pericycle, and remained active throughout lateral root development. This study identifies RBOHs as potentially important players in lateral root development in P. vulgaris. PMID:23221754

  19. Respose of Phaseolus vulgaris L. cultivars to drought stress

    Directory of Open Access Journals (Sweden)

    Amalia Domínguez

    2014-01-01

    Full Text Available Tolerance to drought is a desired cultivars of Phaseolus vulgaris L. The present study aimed to determine the response of 22 cultivars of common bean during the early stages of vegetative development character. The plants were subjected to irrigation at 70% or 20% of field capacity (FC for seven days and the indicators were measured relative water content, stomatal opening, stomatal index, proline content and total phenols in leaves. The data obtained were processed using a principal component analysis and the variables studied were represented by a bivariate graph (biplot. It was possible to group the cultivars based on their response in tolerant, moderately tolerant and susceptible to water stress condition induced by irrigation at 20% FC. Stomatal opening and relative water content were recommended to be used as criteria for selecting cultivars tolerant to water stress indicators bean. Key words: PCA, Phaseolus vulgaris L., proline, water stress

  20. Significance of diet in treated and untreated acne vulgaris

    Directory of Open Access Journals (Sweden)

    Alicja Kucharska

    2016-05-01

    Full Text Available The relationship between diet and acne is highly controversial. Several studies during the last decade have led dermatologists to reflect on a potential link between diet and acne. This article presents the latest findings on a potential impact that diet can have on pathogenesis of acne vulgaris. The association between diet and acne can no longer be dismissed. Compelling evidence shows that high glycemic load diets may exacerbate acne. Dairy ingestion appears to be weakly associated with acne and the roles of omega-3 fatty acids, dietary fiber, antioxidants, vitamin A, zinc and iodine remain to be elucidated. The question of what the impact of diet is on the course of acne vulgaris still remains unclear.

  1. Significance of diet in treated and untreated acne vulgaris.

    Science.gov (United States)

    Kucharska, Alicja; Szmurło, Agnieszka; Sińska, Beata

    2016-04-01

    The relationship between diet and acne is highly controversial. Several studies during the last decade have led dermatologists to reflect on a potential link between diet and acne. This article presents the latest findings on a potential impact that diet can have on pathogenesis of acne vulgaris. The association between diet and acne can no longer be dismissed. Compelling evidence shows that high glycemic load diets may exacerbate acne. Dairy ingestion appears to be weakly associated with acne and the roles of omega-3 fatty acids, dietary fiber, antioxidants, vitamin A, zinc and iodine remain to be elucidated. The question of what the impact of diet is on the course of acne vulgaris still remains unclear.

  2. Pigments content in Сhlorella vulgaris under the influence of the sodium selenite and the ions of metals

    Directory of Open Access Journals (Sweden)

    O. I. Bodnar

    2016-01-01

    Full Text Available We investigated the content of pigments in Chlorella vulgaris Beij. (Chlorophyta under the influence of sodium selenite in concentrations based on Se4+ 0.5, 5.0, 10.0 and 20.0 mg/dm3 during one, three and seven days and under the influence of the simultaneous action of selenite in concentrations 10 mg Se4+/dm3 and ions of Zn2+, Mn2+, Co2+,Cu2+, Fe3+ in concentrations 5.00, 0.25, 0.05, 0.008 and 0.002 mg/dm3over seven days. This research was carried out to establish the conditions for obtaining algal lipidis substance enriched with selenium and biogenic metals in the aquaculture. The content of pigments was determined spectrophotometrically, the cellular walls were separatedby centrifuge in the percoll gradient and investigated microscopically. The pigments content in the Ch. vulgaris increased by 1.5–2.5 times in comparison with the control sample under the influence of 10 mg Se4+/dm3 with and without metal ions, in all variants of experimental influence due to binding of SeО32– with proteins and lipids. We found that selenium was included in all lipid fractions (triacylglycerols, dyacylglycerols, phospholipids, nonetherified fatty acids; the maximum amount of selenium-containing lipids wasrecorded in chloroplasts. The increase in the contents of carotenoids caused by the actions of experimental factors played an exceptional role in the mechanism of antioxidant protection that prevents destruction of chlorophyll and, accordingly, increases its amount in cells. Changes in the functioning of the photosynthetic apparatus of Ch. vulgaris affect the whole complex of metabolic transformation. Thus, the successful cultivation of chlorella, enriched with selenium and biogenic metals, is possible within seven days under the influence of 10 mg Se4+/dm3 and the above-mentioned concentration of metal ions.

  3. [Clinical features and genetics of the ichthyosis vulgaris group].

    Science.gov (United States)

    Traupe, H; Happle, R

    1980-12-11

    Combined application of clinical, genetic and histological criteria in general allows a definite diagnosis of autosomal dominant ichthyosis vulgaris and of X-linked recessive ichthyosis. For differential diagnosis, the following rare syndromes should be considered: ichthyosis bullosa: Refsum syndrome; Jung-Vogel syndrome; ichthyosis with corneal opacity, pili torti and alopecia; ichthyosis with deafness, pili torti and dental anomalies; and ichthyosis with hepatosplenomegaly and cerebellar degeneration.

  4. The morphology and adhesion mechanism of Octopus vulgaris suckers.

    Science.gov (United States)

    Tramacere, Francesca; Beccai, Lucia; Kuba, Michael; Gozzi, Alessandro; Bifone, Angelo; Mazzolai, Barbara

    2013-01-01

    The octopus sucker represents a fascinating natural system performing adhesion on different terrains and substrates. Octopuses use suckers to anchor the body to the substrate or to grasp, investigate and manipulate objects, just to mention a few of their functions. Our study focuses on the morphology and adhesion mechanism of suckers in Octopus vulgaris. We use three different techniques (MRI, ultrasonography, and histology) and a 3D reconstruction approach to contribute knowledge on both morphology and functionality of the sucker structure in O. vulgaris. The results of our investigation are two-fold. First, we observe some morphological differences with respect to the octopus species previously studied (i.e., Octopus joubini, Octopus maya, Octopus bimaculoides/bimaculatus and Eledone cirrosa). In particular, in O. vulgaris the acetabular chamber, that is a hollow spherical cavity in other octopuses, shows an ellipsoidal cavity which roof has an important protuberance with surface roughness. Second, based on our findings, we propose a hypothesis on the sucker adhesion mechanism in O. vulgaris. We hypothesize that the process of continuous adhesion is achieved by sealing the orifice between acetabulum and infundibulum portions via the acetabular protuberance. We suggest this to take place while the infundibular part achieves a completely flat shape; and, by sustaining adhesion through preservation of sucker configuration. In vivo ultrasonographic recordings support our proposed adhesion model by showing the sucker in action. Such an underlying physical mechanism offers innovative potential cues for developing bioinspired artificial adhesion systems. Furthermore, we think that it could possibly represent a useful approach in order to investigate any potential difference in the ecology and in the performance of adhesion by different species.

  5. Acne Vulgaris and Acne Rosacea: An Update in Etiopathogenesis

    OpenAIRE

    Ozlem Ekiz

    2014-01-01

    Acne vulgaris is a chronic inflammatory disease of the pilosebaceous unit, characterized by comedones, papules, pustules, nodules, and scars rarely. The major pathogenic factors are abnormal follicular differentiation and increased ductal cornification, abnormal activity of sebaceous glands, microbial colonization of pilosebaceous units by Propionibacterium acnes and inflammation. Rosacea is a common, chronic inflammatory relapsing skin disorder of the central area of the face characterized b...

  6. Human platelet aggregation inhibitors from thyme (Thymus vulgaris L.).

    Science.gov (United States)

    Okazaki, Kenji; Kawazoe, Kazuyoshi; Takaishi, Yoshihisa

    2002-06-01

    Two antiaggregant compounds, thymol (compound 1) and 3,4,3',4'-tetrahydroxy-5,5'-diisopropyl-2,2'-dimethylbiphenyl (compound 2) were isolated from the leaves of thyme (Thymus vulgaris L.). The structures were determined by (1)H-, (13)C-NMR and mass spectra (MS) studies. These compounds inhibited platelet aggregation induced by collagen, ADP, arachidonic acid (AA) and thrombin except that compound 2 did not inhibit platelet aggregation induced by thrombin.

  7. An unusual central retinal dystrophy associated with ichthyosis vulgaris.

    Science.gov (United States)

    Saatci, O A; Ozbek, Z; Köse, S; Durak, I; Kavukçu, S

    2000-06-01

    A number of ichthyosis syndromes may have retinal abnormalities such as the retinitis pigmentosa-like diffuse rod-cone dystrophy in Refsum's syndrome and the maculopathy in Sjögren-Larsson syndrome. We present two sisters who have an unusual, almost identical, bilaterally symmetric central retinal dystrophy associated with ichthyosis vulgaris in the absence of other systemic disorders. We believe that this dystrophy has not been previously described in patients with any of the known varieties of ichthyosis.

  8. Lupus vulgaris of the popliteal fossa: a delayed diagnosis.

    Science.gov (United States)

    Altunay, Ilknur Kivanc; Kayaoglu, Semra; Ekmekci, Tugba Rezan; Kutlu, Safiye; Arpag, Esra Saygin

    2007-07-13

    Lupus vulgaris (LV) is the most common form of cutaneous tuberculosis. It commonly presents on the head and neck regions. The diagnosis may be difficult when LV occurs at unexpected regions or in unusual clinical forms. Sometimes special stains for the organism and mycobacterial cultures may be negative. Nevertheless, it is usually possible to reach the correct diagnosis of LV using clinical and histopathological findings. But at times, a therapeutic trial with antitubercular agents may be required.

  9. Anti-androgens in treatment of acne vulgaris.

    Science.gov (United States)

    Amer, M; Ramadan, A; Abdel Monem, A

    1985-10-01

    Seventy-five women suffering from acne vulgaris classified into three groups according to the grade of the disease. Anti-androgenic tablets cyproterone acetate (CPA), were given in three cycles. The total number of patients whose condition improved was 52% after the first cycle, 55% after the second cycle, and 81.3% after the third cycle. No serious side effects were encountered. CPA is a suitable therapeutic modility for AV in women who use contraceptives.

  10. The morphology and adhesion mechanism of Octopus vulgaris suckers.

    Directory of Open Access Journals (Sweden)

    Francesca Tramacere

    Full Text Available The octopus sucker represents a fascinating natural system performing adhesion on different terrains and substrates. Octopuses use suckers to anchor the body to the substrate or to grasp, investigate and manipulate objects, just to mention a few of their functions. Our study focuses on the morphology and adhesion mechanism of suckers in Octopus vulgaris. We use three different techniques (MRI, ultrasonography, and histology and a 3D reconstruction approach to contribute knowledge on both morphology and functionality of the sucker structure in O. vulgaris. The results of our investigation are two-fold. First, we observe some morphological differences with respect to the octopus species previously studied (i.e., Octopus joubini, Octopus maya, Octopus bimaculoides/bimaculatus and Eledone cirrosa. In particular, in O. vulgaris the acetabular chamber, that is a hollow spherical cavity in other octopuses, shows an ellipsoidal cavity which roof has an important protuberance with surface roughness. Second, based on our findings, we propose a hypothesis on the sucker adhesion mechanism in O. vulgaris. We hypothesize that the process of continuous adhesion is achieved by sealing the orifice between acetabulum and infundibulum portions via the acetabular protuberance. We suggest this to take place while the infundibular part achieves a completely flat shape; and, by sustaining adhesion through preservation of sucker configuration. In vivo ultrasonographic recordings support our proposed adhesion model by showing the sucker in action. Such an underlying physical mechanism offers innovative potential cues for developing bioinspired artificial adhesion systems. Furthermore, we think that it could possibly represent a useful approach in order to investigate any potential difference in the ecology and in the performance of adhesion by different species.

  11. [Correction of experimental anxiety behavior by meadowsweet (Filipendula vulgaris) extracts].

    Science.gov (United States)

    Vengerovskiĭ, A I; Suslov, N I; Kaĭgorodtsev, A V

    2011-01-01

    Influence of aqueous and aqueous-ethanol extracts of meadowsweet (Filipendula vulgaris) on the behavior of albino mice in anxiety models has been studied. It is established that the aqueous and 95% aqueous ethanol extracts of meadowsweet produce the most expressed anxiolytic action in the elevated plus-maze, open field, and emotional reaction tests. The anxiolytic activity of these extracts exceeds that of the reference valerian extract.

  12. Nail changes in female pemphigus vulgaris patients on immunosuppressive therapy

    OpenAIRE

    M.M. El-Komy, MD; D.M. Abdel Halim, MD; N. Samir, MD; R.A. Hegazy, MD; H.I. Gawdat, MD; S.A. Shoeb, MD

    2015-01-01

    Background: Pemphigus vulgaris (PV) patients receiving immunosuppressive therapy may develop nail alterations resulting from infection, skin disorder, or drug regimen. Objective: This study aims to describe nail changes in PV female patients receiving immunosuppressive therapy and to report the frequency of associated fungal and bacterial growth in the patients’ nails. Methods: Twenty-five female PV patients who had at least one acquired finger or toenail abnormality and had been admini...

  13. Effects of box handle position and carrying range on bi-manual carrying capacity for females.

    Science.gov (United States)

    Wu, Swei-Pi; Loiu, Yi; Chien, Te Hong

    2015-01-01

    This study utilizes a psychophysical approach to examine the effects on carrying capacity for bi-manual carrying tasks involving different handle positions and carrying ranges. A total of 16 female subjects participated in the experiment in groups of two people, and each group of subjects performed the tasks in a random order with 12 different combinations of carrying task. The independent variables are handle position (upper, middle, lower) and carrying range (F-F: floor height carried to floor height, F-W: floor height carried to waist height, W-W: waist height carried to waist height, W-F: waist height carried to floor height), the dependent variable is the maximum acceptable carried weight (MAWC), heart rate (HR), and the rating of perceived exertion (RPE). The results show that the handle position has a significant effect on MAWC and overall RPE but no significant effect on HR. Carrying range has a significant effect on the MAWC and HR, but no significant effect on overall HR. The handle position and carrying range have a significant interaction on the MAWC and HR. The RPE for different body parts shows significant differences, and the hands feel the most tired. Overall, this study confirms that the lower handle position with the W-W carrying range is the best combination for a two-person carrying task.

  14. Vitreoscilla hemoglobin gene ( vgb) improves lutein production in Chlorella vulgaris

    Science.gov (United States)

    Ma, Ruijuan; Lin, Xiangzhi

    2014-03-01

    Vitreoscilla hemoglobin is an oxygen-binding protein that promotes oxygen delivery and reduces oxygen consumption under low oxygen conditions to increase the efficiency of cell respiration and metabolism. In this study, we introduced a Vitreoscilla hemoglobin gene ( vgb) into Chlorella vulgaris by Agrobacterium tumefaciens -mediated transformation (ATMT). PCR analysis confirmed that the vgb gene was successfully integrated into the Chlorella vulgaris genome. Analysis of biomass obtained in shake flasks revealed transformant biomass concentrations as high as 3.28 g/L, which was 38.81% higher than that of the wild-type strain. Lutein content of transformants also increased slightly. Further experiments recovered a maximum lutein yield of 2.91 mg/L from the transformants, which was 36.77% higher than that of the wild-type strain. The above results suggest that integrated expression of the vgb gene may improve cell growth and lutein yield in Chlorella vulgaris, with applications to lutein production from Chlorella during fermentation.

  15. Photon up-conversion increases biomass yield in Chlorella vulgaris.

    Science.gov (United States)

    Menon, Kavya R; Jose, Steffi; Suraishkumar, Gadi K

    2014-12-01

    Photon up-conversion, a process whereby lower energy radiations are converted to higher energy levels via the use of appropriate phosphor systems, was employed as a novel strategy for improving microalgal growth and lipid productivity. Photon up-conversion enables the utilization of regions of the solar spectrum, beyond the typical photosynthetically active radiation, that are usually wasted or are damaging to the algae. The effects of up-conversion of red light by two distinct sets of up-conversion phosphors were studied in the model microalgae Chlorella vulgaris. Up-conversion by set 1 phosphors led to a 2.85 fold increase in biomass concentration and a 3.2 fold increase in specific growth rate of the microalgae. While up-conversion by set 2 phosphors resulted in a 30% increase in biomass and 12% increase in specific intracellular neutral lipid, while the specific growth rates were comparable to that of the control. Furthermore, up-conversion resulted in higher levels of specific intracellular reactive oxygen species in C. vulgaris. Up-conversion of red light (654 nm) was shown to improve biomass yields in C. vulgaris. In principle, up-conversion can be used to increase the utilization range of the electromagnetic spectrum for improved cultivation of photosynthetic systems such as plants, algae, and microalgae.

  16. Photosynthetic and cellular toxicity of cadmium in Chlorella vulgaris.

    Science.gov (United States)

    Ou-Yang, Hui-Ling; Kong, Xiang-Zhen; Lavoie, Michel; He, Wei; Qin, Ning; He, Qi-Shuang; Yang, Bin; Wang, Rong; Xu, Fu-Liu

    2013-12-01

    The toxic effects of cadmium (Cd) on the green alga Chlorella vulgaris were investigated by following the response to Cd of various toxicity endpoints (cell growth, cell size, photochemical efficiency of PSII in the light or Φ(PSII), maximal photochemical efficiency or Fv/Fm, chlorophyll a fluorescence, esterase activity, and cell viability). These toxicity endpoints were studied in laboratory batch cultures of C. vulgaris over a long-term 96-h exposure to different Cd concentrations using flow cytometry and pulse amplitude modulated fluorometry. The sequence of sensitivity of these toxicity endpoints was: cell yield > Φ(PSII) ≈ esterase activity > Fv/Fm > chlorophyll a fluorescence ≈ cell viability. It is shown that cell apoptosis or cell death only accounted for a minor part of the reduction in cell yield even at very high algistatic free Cd²⁺ concentrations, and other mechanisms such as blocked cell divisions are major contributors to cell yield inhibition. Furthermore, cadmium may affect both the electron donors and acceptors of the electron transport chain at high free Cd²⁺ concentration. Finally, the resistance of cells to cell death was size-dependent; medium-sized cells had the highest toxicity threshold. The present study brings new insights into the toxicity mechanisms of Cd in C. vulgaris and provides a detailed comparison of the sensitivity of various Cd toxicity endpoints.

  17. Ammonium reduces chromium toxicity in the freshwater alga Chlorella vulgaris.

    Science.gov (United States)

    Liu, Jingqing; Sun, Zhengqi; Lavoie, Michel; Fan, Xiaoji; Bai, Xiaocui; Qian, Haifeng

    2015-04-01

    The aim of the present study was to investigate the protective effect of ammonium (NH4 (+)) on Cr toxicity to the freshwater alga Chlorella vulgaris. We followed an array of cellular functions and biomolecules in C. vulgaris cells exposed to 50 or 100 μM Cr at three different initial NH4 (+) concentrations (0.5, 3, and 10 mM). The results showed that Cr strongly inhibited cell yield of C. vulgaris, but 10 mM NH4 (+) could decrease by more than two-fold Cr toxicity on cell yield compared to exposure to 0.5 mM NH4 (+). Cr toxicity on gene transcripts and cellular substructure was also much lower at high than at low NH4 (+). Our results suggest that this protecting effect of NH4 (+) on intracellular Cr toxicity could be due to several factors, such as enhance uptake of phosphorus, increase in C and N assimilation efficiency, and increase transcription of photosynthesis-related genes.

  18. Growth of Chlorella vulgaris and associated bacteria in photobioreactors.

    Science.gov (United States)

    Lakaniemi, Aino-Maija; Intihar, Veera M; Tuovinen, Olli H; Puhakka, Jaakko A

    2012-01-01

    The aim of this study was to test three flat plate photobioreactor configurations for growth of Chlorella vulgaris under non-axenic conditions and to characterize and quantify associated bacterial communities. The photobioreactor cultivations were conducted using tap water-based media to introduce background bacterial population. Growth of algae was monitored over time with three independent methods. Additionally, the quantity and quality of eukaryotes and bacteria were analysed using culture-independent molecular tools based on denaturing gradient gel electrophoresis (PCR-DGGE) and quantitative polymerase chain reaction (QPCR). Static mixers used in the flat plate photobioreactors did not generally enhance the growth at the low light intensities used. The maximum biomass concentration and maximum specific growth rate were 1.0 g l(-1) and 2.0 day(-1) respectively. Bacterial growth as determined by QPCR was associated with the growth of C. vulgaris. Based on PCR-DGGE, bacteria in the cultures mainly originated from the tap water. Bacterial community profiles were diverse but reproducible in all flat plate cultures. Most prominent bacteria in the C. vulgaris cultures belonged to the class Alphaproteobacteria and especially to the genus Sphingomonas. Analysis of the diversity of non-photosynthetic microorganisms in algal mass cultures can provide useful information on the public health aspects and unravel community interactions.

  19. Metastasized squamous cell carcinoma developed on lupus vulgaris.

    Science.gov (United States)

    Pătraşcu, V; Georgescu, Claudia Valentina; Tănase, Loredana Elena; Mogoantă, S S

    2008-01-01

    Lupus vulgaris (LV) is the most frequent cutaneous tuberculosis, representing more than 55% of the tuberculoses with this location. Malignization can occur after a long latency (10-30 years), in 1-2% of the cases, and it is mainly in squamous cell carcinoma. The histological exam is highly important in the observation of neoplasic transformations. The authors present a 59-years-old female patient, from the rural environment, working as a farmer, with lupus vulgaris developing since her first childhood years. It started at the age of 2 years, at the right ear lobule, after the empiric perforation for earrings. The evolution was progressive, eccentric, interesting the pinna and the right cheek in the meanwhile. At the first examination, in 2002, a diffuse mass of red-yellowish infiltration was found at the level of the right ear and the right cheek. In the following two years, an ulcero-vegetating tumor developed at the level of the right ear lobule, accompanied by the presence of a right retromandibular adenopathy, of about 1 cm, which was proved by the histopathologic exam to be a squamous cell carcinoma developed from a lupus vulgaris. After scraping out the right retromandibular ganglion, detected by palpation, a histological exam showed ganglion metastasis.

  20. Detection of apoptosis in pemphigus vulgaris by TUNEL technique*

    Science.gov (United States)

    Cuevas-Gonzalez, Juan Carlos; Vega-Memíje, Maria Elisa; García-Vázquez, Francisco Javier; Aguilar-Urbano, Marco António

    2016-01-01

    Background Pemphigus is part of a group of blistering diseases that affect the skin and mucous membranes. Based on its autoimmune origin, autoantibodies develop in pemphigus that are directed toward cell surface components of keratinocytes. However, some data cannot be explained, such as the lack of a relationship between autoantibody levels and the severity of clinical manifestations, treatment resistance, the presence of inflammatory infiltrates and the potential occurrence of apoptosis as determinants of vesicle formation. Objective To examine the presence of apoptosis in pemphigus vulgaris by TUNEL technique. Methods In this cross-sectional study, we selected 15 paraffin-embedded tissues from subjects who were diagnosed with pemphigus vulgaris by hematoxylin and eosin staining. The samples were subjected to TUNEL assay and examined under an Olympus BX61 fluorescence microscope. Positivity was categorized dichotomously, and the statistical analysis was performed using the X2 test. Results Positivity was observed in basal layer cells in 14 (93.3%) cases. In 13 (86.7%) of the positive cases, we noted espinosum and granular layers that formed the blister roof, and in 12 cases (80%), positive acantholytic cells were observed. Conclusions TUNEL positivity was observed in pemphigus vulgaris, implicating apoptosis in the pathophysiology of this condition, which can help guide the development of apoptotic blockers as therapeutics. PMID:27438195

  1. Profile of acne vulgaris-A hospital-based study from South India

    Directory of Open Access Journals (Sweden)

    Adityan Balaji

    2009-01-01

    Full Text Available Background: Acne vulgaris is believed to be the most common disease of the skin. There is no Indian study on the profile of acne vulgaris, markers of severe forms of acne vulgaris and a possible correlation between acne vulgaris and markers of androgenicity in females. Aim: To study the profile of acne vulgaris, its seasonal variation, relationship with smoking and possible correlation between acne vulgaris and markers of androgenicity in females. Methods: The study was conducted between August 2006 and June 2008. All patients with acne vulgaris who consented to participate in the study were included. The parameters evaluated included age, gender, age of onset, duration of lesions, site of lesions, grade, relation with menstrual cycle, markers of androgenicity, number of acne lesions such as comedones, papules pustules and nodules, number and site of post-acne scarring, post-acne hyperpigmentation, seasonal variation and history of smoking. Results: A total of 309 patients with acne vulgaris were included in the study. The frequency of acne vulgaris in our study was 1.068%. Mean age of the study group was 19.78 years. Male to female ratio was 1.25:1. The most common age group involved was 16 to 20 years (59.8%. Mean age of onset was 15.97 years. Face was involved in all the patients, followed by back (28.2%, chest (20.1%, neck (9.4% and arms (10%. In the older age groups, women were more likely to report having acne vulgaris than men ( P = 0.01. The closed comedones outnumbered open comedones by a factor of 4.9:1. A total of 186 patients (60.2% had grade 1 acne vulgaris, 85 (27.5% had grade 2 acne, 8 (2.6% had grade 3 acne and 30 (9.7% had grade 4 acne vulgaris. There was a higher incidence of scarring (39.5% and post-acne hyperpigmentation (24.6% in our study. In female patients, 57.7% had premenstrual flare and 12.4% had cutaneous markers of androgenicity. There was no association between severity of acne vulgaris and other markers of

  2. Optimal growth trajectories with finite carrying capacity.

    Science.gov (United States)

    Caravelli, F; Sindoni, L; Caccioli, F; Ududec, C

    2016-08-01

    We consider the problem of finding optimal strategies that maximize the average growth rate of multiplicative stochastic processes. For a geometric Brownian motion, the problem is solved through the so-called Kelly criterion, according to which the optimal growth rate is achieved by investing a constant given fraction of resources at any step of the dynamics. We generalize these finding to the case of dynamical equations with finite carrying capacity, which can find applications in biology, mathematical ecology, and finance. We formulate the problem in terms of a stochastic process with multiplicative noise and a nonlinear drift term that is determined by the specific functional form of carrying capacity. We solve the stochastic equation for two classes of carrying capacity functions (power laws and logarithmic), and in both cases we compute the optimal trajectories of the control parameter. We further test the validity of our analytical results using numerical simulations.

  3. Optimal growth trajectories with finite carrying capacity

    Science.gov (United States)

    Caravelli, F.; Sindoni, L.; Caccioli, F.; Ududec, C.

    2016-08-01

    We consider the problem of finding optimal strategies that maximize the average growth rate of multiplicative stochastic processes. For a geometric Brownian motion, the problem is solved through the so-called Kelly criterion, according to which the optimal growth rate is achieved by investing a constant given fraction of resources at any step of the dynamics. We generalize these finding to the case of dynamical equations with finite carrying capacity, which can find applications in biology, mathematical ecology, and finance. We formulate the problem in terms of a stochastic process with multiplicative noise and a nonlinear drift term that is determined by the specific functional form of carrying capacity. We solve the stochastic equation for two classes of carrying capacity functions (power laws and logarithmic), and in both cases we compute the optimal trajectories of the control parameter. We further test the validity of our analytical results using numerical simulations.

  4. BOOKLET TO INSTITUTO PEDAGOGICO NACIONAL TEACHERS ABOUT SYMBIOSIS AND PROCESSES ON BIOTECHNOLOGY: THE BIOFERTILIZER Rhizobium sp IN Phaseolus vulgaris WITH ALTERNATIVE TO SYMBIOSIS FOR Phaseolus vulgaris

    Directory of Open Access Journals (Sweden)

    María Camila Quevedo Rubiano

    2016-09-01

    Full Text Available This article presents the results of the thesis carried out in the research group of Biotechnology Teaching in Colombia, with the aim of providing teachers of Biology of Instituto Pedagogico Nacional a booklet that can strengthen the teaching of biotechnology processes using Rhizobium sp reduction of chemical fertilizers and symbiosis with Phaseolus vulgaris.   The booklet contains a proposal of practical activities that enable teachers of this institution to use spaces like the farm, enabling to teach biotechnology related to agronomy. Therefore, for this project was considered two Biological and Pedagogical approaches, the first is within the analytical empirical paradigm in the process of microbiological characterization of Rhizobium and their Biofertilizing ability in beans; and the teaching approach within the design of a booklet that includes the findings of this study as a contribution to the reduction of chemical fertilizers school farm. In order to have a complete analysis of the work it was subjected to quantitative and qualitative methods.   This biotech practice is included in the booklet showing in bioassays that bacteria has biofertilizer without inhibiting potential symbiosis, and that research and teaching biological concepts from scientific expertise can be promoted in Biology class for students to understand its context in a significant way, to be used in different levels of education; also it is a teaching strategy.

  5. Carry Select Adder Circuit with A Successively Incremented Carry Number Block

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    Deepak

    2014-04-01

    Full Text Available This paper reports a conditional carry select (CCS adder circuit with a successively-incremented-carry-number block (SICNB structure for low-voltage VLSI implementation. Owing to the successively-incremented-carry-number block (SICNB structure, the new 16-bit SICNB CCS adder provides a 37% faster speed as compared to the conventional conditional Carry select adder based on the SPICE results

  6. An Optical Carry Chain Fast Adder

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    D. Al-Dabass

    1994-12-01

    Full Text Available A significant problem in Arithmetic Unit design and particularly for systolic arrays remains the speed attainable in achieving high speed addition. The root of the problem is carry propagation and a method is presented which is relatively independent of word length. The problem is addressed by the description of a suggested radical design involving a hybrid optical and electronic approach. The method of carry chain addition through pass gates is explained and a suggested implementation utilising Fabry-Perott resonators, optical waveguides and voltage controlled couplers is described. The design is suitable for n-stage modular expansion.

  7. Preliminary study of the green algae chlorella (Chlorella vulgaris) for control on the root-knot nematode (Meloidogyne arenaria) in tomato plants and ectoparasite Xiphinema indexin grape seedlings.

    Science.gov (United States)

    Choleva, B; Bileva, T; Tzvetkov, Y; Barakov, P

    2005-01-01

    The alternative ecological methods require investigation of many organo-biological means for plant protection against dangerous root parasites such as root-knot nematode Meloidogyne arenaria and some ectoparasites (Xiphinema index). The Bulgarian organic product - dry extract of green alga Chlorella vulgaris ("The Golden Apple"-Plamen Barakov) is the latest product, which in comparative aspect gives the best results. Series of laboratory and pot experiments are carried out with tomato (cv. Bele and cv. Ideal) and grape seedlings (cv. Cabernet Sauvignon). Different dosages of Chlorella from 0.5 g to 2 g per plant/pot are investigated. The first results show that even low dosages had double effect - on the one hand they suppress the parasite development and on the other hand they strongly stimulate plant growing. The very important conclusion is that Chlorella vulgaris ignores the negative influence of M. arenaria and X. index. These results give us opportunity for future model and field investigations of Chlorella vulgaris with the aim of its practical application.

  8. EVALUATION OF THE FLOCCULATION EFFICIENCY OF Chlorella vulgaris MEDIATED BY Moringa oleifera SEED UNDER DIFFERENT FORMS: FLOUR, SEED CAKE AND EXTRACTS OF FLOUR AND CAKE

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    C. M. L. Lapa Teixeira

    Full Text Available Abstract Flocculation as a pre-separation method can help make production of biodiesel from microalgae economically feasible. In a previous study, Moringa oleifera seed flour (1 g.L-1 was shown to be a very efficient flocculant for Chlorella vulgaris, a microalga with high potential for biodiesel production. In this study, several aspects of C vulgaris flocculation mediated by Moringa were investigated in order to optimize the separation of this biomass. Flocculation efficiency was the same with seeds from different origins and lots. The stationary growth stage was best for harvesting C vulgaris cells to carry out flocculation efficiently (93%. The use of flour extracts and cake extracts generated the best cost-benefit ratio (flocculation efficiency from 78 to 97% with a saving in mass of seed of 75%. The highest efficiency was reached with extracts prepared with seawater and NaCl solutions which have high salt concentration. Reasonable stability of the extract allows its use for up to two weeks, provided it is kept at low temperature (4 ºC.

  9. 寻常型银屑病诱发因素分析%Causative Factors of Psoriasis Vulgaris

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    魏瑾; 刘梅; 肖汀; 曲乐; 陈洪铎; 何春涤

    2011-01-01

    Objective To investigate the causative factors of psoriasis vulgaris. Methods A total of 426 psoriasis vulgaris patients and 500 healthy subjects were enrolled. Through the method of case control study, single factor analysis and multi-factor analysis on ten factors of the case group and the control group subjects were carried out. Results It was showed by single factor analysis that damp conditions, infection, wound, drinking, fish and shrimp, drugs, mental stress and smoke were all among the risk factors of psoriasis with significant differences between the case and the control group ( P < 0.05 ) . Multi - factor analysis revealed that all the factors mentioned above contributed to the pathogenesis of psoriasis vulgaris except for drugs ( P <0.05 ) . Conclusion A number of environmental factors contribute to the development of psoriasis vulgaris.%目的 探讨寻常型银屑病的诱发因素.方法 选取426例寻常型银屑病患者(病例组)和500例健康者(对照组).应用病例对照研究方法,对两组受检者的10种因素进行单因素和多因素分析.结果 单因素分析显示,受潮、感染、外伤、饮酒、食鱼虾、药物、精神紧张及吸烟的患者在病例组、对照组中差异有统计学意义(P<0.05).多元Logistic回归分析显示,除了药物外,其他因素均是寻常型银屑病的诱发因素(P<0.05).结论 多种环境因素在寻常型银屑病的发生、发展中起重要作用.

  10. Sensory analysis of beans (Phaseolus vulgaris

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    Sanz-Calvo M.

    1999-01-01

    Full Text Available The methodology of sensory profiling constitutes the basis of a descriptive quantitative analysis, defining a product with the minimum number of words and with maximum efficiency, using a precise tasting sheet, which can be reproduced and is understood by all. In this work, the texture profiling for different bean varieties that are characteristic of the Spanish market was carried out. Optimum conditions for samples and a tasting card were established, and a panel was trained. The texture profile results show significant differences amongst varieties and even amongst different origins for the same variety.

  11. Specific filaggrin mutations cause ichthyosis vulgaris and are significantly associated with atopic dermatitis in Japan.

    Science.gov (United States)

    Nomura, Toshifumi; Akiyama, Masashi; Sandilands, Aileen; Nemoto-Hasebe, Ikue; Sakai, Kaori; Nagasaki, Akari; Ota, Mitsuhito; Hata, Hiroo; Evans, Alan T; Palmer, Colin N A; Shimizu, Hiroshi; McLean, W H Irwin

    2008-06-01

    Mutations in the gene encoding filaggrin (FLG) have been identified as the cause of ichthyosis vulgaris (IV) and shown to be major predisposing factors for atopic dermatitis (AD). However, these studies have been mainly carried out in European populations. In early 2007, we identified two Oriental-specific FLG mutations in four Japanese families with IV and reported that filaggrin mutations were also significant predisposing factors for AD in Japan. However, the frequency of FLG mutations observed in our Japanese AD cohort (5.6%), was much lower than that seen in Europeans (up to 48%). Here, we studied a further seven Japanese families with IV and identified two additional nonsense mutations in FLG, S2889X, and S3296X. We found that more than 20% of patients in our Japanese AD case series carry FLG mutations, and there is significant statistical association between the four mutations and AD (chi(2) P=8.4 x 10(-6); heterozygote odds ratio 7.57, 95% CI 2.84-23.03). These data emphasize that skin-barrier impairment due to reduced filaggrin expression plays an important role in the pathogenesis of AD and sheds further light on the genetic architecture of atopy in Japan.

  12. Iron and zinc retention in common beans (Phaseolus vulgaris L. after home cooking

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    Lucia M. J. Carvalho

    2012-02-01

    Full Text Available Background : According to the World Health Organization (WHO, iron, iodine, and Vitamin A deficiencies are the most common forms of malnutrition, leading to severe public health consequences. The importance of iron and zinc in human nutrition and the number of children found to be deficient in these nutrients make further studies on retention in cooked grains and cooked bean broth important. Objectives : This work aimed to evaluate iron and zinc retention in six common bean (Phaseolus vulgaris L. cultivars under the following conditions: raw beans, regular pot cooking, pressure cooking, with and without previous water soaking, and broth. Design : Determination of iron and zinc content in the raw, cooked bean grains and broth samples was carried out by Inductively Coupled Plasma (ICP Optical Emission Spectrometry (Spectro Analytical Instrument – Spectroflame P. All experiments and analyses were carried out in triplicate. Results : Overall, regardless of the cooking method, with or without previous water soaking, the highest zinc concentration was found in the cooked bean grains. However, pressure cooking and previous water soaking diminished iron retention in the cooked grains, while increasing it in the bean broth. Conclusion : The common bean was confirmed to be an excellent source of iron and zinc for human consumption, and it was suggested that beans should be consumed in a combined form, i.e. grain with bean broth.

  13. Error propagation in energetic carrying capacity models

    Science.gov (United States)

    Pearse, Aaron T.; Stafford, Joshua D.

    2014-01-01

    Conservation objectives derived from carrying capacity models have been used to inform management of landscapes for wildlife populations. Energetic carrying capacity models are particularly useful in conservation planning for wildlife; these models use estimates of food abundance and energetic requirements of wildlife to target conservation actions. We provide a general method for incorporating a foraging threshold (i.e., density of food at which foraging becomes unprofitable) when estimating food availability with energetic carrying capacity models. We use a hypothetical example to describe how past methods for adjustment of foraging thresholds biased results of energetic carrying capacity models in certain instances. Adjusting foraging thresholds at the patch level of the species of interest provides results consistent with ecological foraging theory. Presentation of two case studies suggest variation in bias which, in certain instances, created large errors in conservation objectives and may have led to inefficient allocation of limited resources. Our results also illustrate how small errors or biases in application of input parameters, when extrapolated to large spatial extents, propagate errors in conservation planning and can have negative implications for target populations.

  14. Increased carrying capacity with perennial forage kochia

    Science.gov (United States)

    Carrying capacity can be increased on grass-dominated rangeland pastures by including perennial forage kochia (Kochia prostrata) as one of the plant components. The objectives of the study reported here were to compare the differences of traditional winter pastures versus pastures with forage kochi...

  15. Antibacterial activity of Thymus vulgaris essential oil alone and in combination with other essential oils

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    MAHENDRA RAI

    2012-07-01

    Full Text Available Kon K, Rai M. 2012. Antibacterial activity of Thymus vulgaris essential oil alone and in combination with other essential oils. Nusantara Bioscience 4: 50-56. Essential oils (EOs from plants represent an alternative approach in combating antibiotic-resistant bacteria. One of the EOs with proven antibacterial properties is Thymus vulgaris EO. The purpose of the present work was to investigate in vitro antibacterial activity of T. vulgaris EO alone and in combination with other EOs. The activity of T. vulgaris EO was screened in combination with 34 EOs against Staphylococcus aureus and Escherichia coli by disk diffusion method; then the most effective combinations were evaluated by broth microdilution method. Against S. aureus the synergistic effect was found in combination of T. vulgaris and Cinnamomum zeylonicum EOs with fractional inhibitory concentration (FIC index of 0.26; Juniperus communis and Picea abies EOs showed additive effect (FIC indexes were 0.74 and 0.78, respectively. Combination of T. vulgaris EO with Aniba rosaeodora and Melissa officinalis EOs demonstrated synergistic effect against E. coli (FIC indexes were 0.23 and 0.34, respectively; combination of T. vulgaris and Mentha piperita EOs was additive (FIC index 0.55. Therefore, combining T. vulgaris EO with other EOs has potential in further enhancing its antibacterial properties.

  16. High social phobia frequency and related disability in patients with acne vulgaris.

    Science.gov (United States)

    Bez, Yasin; Yesilova, Yavuz; Kaya, Mehmet Cemal; Sir, Aytekin

    2011-01-01

    Acne is an easily recognizable abnormality which may cause some adverse psychosocial consequences. We aimed to determine the social phobia frequency, social anxiety level, and disease related disability in a group of acne vulgaris patients. One-hundred and forty acne vulgaris patients and 98 healthy control subjects were included in the study. Acne severity was determined by the Global Acne Grading System (GAGS). A psychiatrist interviewed each participant and the Liebowitz Social Anxiety Scale (LSAS) was administered to all participants, who also completed the Hospital Anxiety and Depression Scale (HADS) and Sheehan Disability Scale (SDS). Social phobia was diagnosed in 45.7% of acne vulgaris patients and in 18.4% of control subjects. Acne vulgaris patients demonstrated higher performance avoidance and total avoidance scores in LSAS than controls. Acne vulgaris patients without social phobia had higher scores in HADS and LSAS than the acne vulgaris patients with social phobia. They were more disabled in their occupational, social, and familial lives. Social phobia diagnosis predicted disability at work, whereas education level predicted the disability in family life of acne vulgaris patients. Social phobia seems to be a common psychiatric comorbidity which may give rise to some additional disability among acne vulgaris patients.

  17. Insect resistance to sugar beet pests mediated by a Beta vulgaris proteinase inhibitor transgene

    Science.gov (United States)

    We transformed sugar beet (Beta vulgaris) hairy roots and Nicotiana benthamiana plants with a Beta vulgaris root gene (BvSTI) that codes for a serine proteinase inhibitor. BvSTI is a root gene cloned from the F1016 breeding line that has moderate levels of resistance to the sugar beet root maggot ...

  18. Climatotherapy at the dead sea for pediatric-onset psoriasis vulgaris.

    Science.gov (United States)

    Ben-Amitai, Dan; David, Michael

    2009-01-01

    Climatotherapy at the Dead Sea is highly effective and safe for the treatment of psoriasis vulgaris in adults. We examine the efficacy and safety of climatotherapy at the Dead Sea in children with psoriasis vulgaris. More than 75% improvement in the Psoriasis Area and Severity Index was noted in 35.3% of the patients. None of the patients had side effects.

  19. Cardiac surgery in a patient with pemphigus vulgaris: anesthetic and surgical considerations.

    Science.gov (United States)

    Takahashi, Mitsuko; Rhee, Amanda J; Rakasi, Raghuveer R; Yildirim, Asu; Silvay, George; Reddy, Ramachandra C

    2014-03-01

    Pemphigus vulgaris is an autoimmune disorder that causes blistering of the skin and mucous membranes. We present a patient with pemphigus vulgaris who required combined coronary artery bypass grafting and mitral valve repair. The challenges that we faced and modifications to the technique required in this situation are described and reviewed.

  20. A hermetic self-sustained microbial solar cell based on Chlorella vulgaris and a versatile charge transfer chain

    Science.gov (United States)

    Pan, Keliang; Zhou, Peijiang

    2015-10-01

    A hermetic noble-metal-free membrane-less microbial solar cell (MSC) is established. The substances decomposition and regeneration in this MSC are carried out only by Chlorella vulgaris simultaneously. The conversion of metabolism types of C. vulgaris is controlled only by illumination. By using a pleiotropic redox mediator and a cupric hexacyanoferrate modified cathode, a two-phase three-stage charge transfer chain is formed. Through this pathway, the one microorganism self-sustained system gets a long-term power output up to 0.04773 mW/cm2 at 0.423 V without any material exchange with external, which is 50 times higher than that obtained from the original system. Benefiting from this electron buffer system, the battery will achieve an electricity generation in both light and dark conditions. There is almost no consumption of any substrates throughout the stabilized process, and no more additions are required. This maintenance-free and extremely inexpensive reactor with a simple structure and a long service life demonstrates the possibility of combining the microbial, chemical and photo cells.

  1. Characterization of biosynthesized gold nanoparticles from aqueous extract of Chlorella vulgaris and their anti-pathogenic properties

    Science.gov (United States)

    Annamalai, Jayshree; Nallamuthu, Thangaraju

    2015-06-01

    In this study, biosynthesis of self-assembled gold nanoparticles (GNPs) was accomplished using an aqueous extract of green microalga, Chlorella vulgaris. The optical, physical, chemical and bactericidal properties of the GNPs were investigated to identify their average shape and size, crystal nature, surface chemistry and toxicity, via UV-visible spectroscopy, scanning electron microscopy, transmission electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy and antimicrobial activity. The sizes of the spherical self-assembled cores of the synthesized GNPs ranged from 2 to 10 nm. The XRD patterns showed a (111) preferential orientation and the crystalline nature of the GNPs. The results of the FTIR analysis suggested that the peptides, proteins, phenol and flavonoid carried out the dual function of effective Au III reduction and successful capping of the GNPs. Human pathogen Candida albicans and Staphylococcus aureus were susceptible to synthesized aqueous GNPs. Thus, biosynthesis, stabilization and self-assembly of the GNPs by Chlorella vulgaris extract can be an example of green chemistry and effective drug in the medicinal field.

  2. A comparative effectiveness research of azathioprine and cyclophosphamide on the clinical and serological response in pemphigus vulgaris

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    Kabir Sardana

    2016-01-01

    Full Text Available Context: A prospective study was carried out to examine the efficacy of cyclophosphamide and azathioprine in pemphigus vulgaris. Aims: To compare the clinical and serological effect of azathioprine and cyclophosphamide in pemphigus patients. Materials and Methods: Prospective, institutional based study was conducted twenty-one patients of pemphigus vulgaris were initiated on either azathioprine (n = 9 or cyclophosphamide (n = 7 in addition to prednisolone and were evaluated clinically (mucosal and cutaneous severity and serologically enzyme-linked immunosorbent assay (ELISA at 0, 3 and 6 months. Results: Azathioprine had a slower onset of action with a statistically significant improvement seen by 6 months (P = 0.016. Cyclophosphamide had a faster onset of action (3 months though there was no statistical difference in the efficacy between the two at the end of 6 months. The (RonT was 33.3-44.4% for azathioprine and 28.8-42.9% for cyclophosphamide at 6 months. Though ELISA had a high sensitivity and specificity for diagnosis, as a tool for assessing therapeutic response a significant decrease was seen only till 3 months. This was restricted to Dsg1 for the azathioprine group and both Dsg3 and Dsg1 levels for the cyclophosphamide group. There were two deaths, both in the cyclophosphamide group. Conclusions: Azathiorpine and cyclophosphamide are equally effective for mucosal and cutaneous disease in pemphigus after 6 months of therapy. Dsg ELISA is useful for diagnosis of pemphigus but is not a useful tool for monitoring response to therapy.

  3. Characterization of bean (Phaseolus vulgaris L.) ecotype "Fagiolo occhio nero di Oliveto Citra" using agronomic, biochemical and molecular approaches.

    Science.gov (United States)

    Zaccardelli, Massimo; Pentangelo, Alfonso; Tripodi, Pasquale

    2013-09-15

    Common bean (Phaseolus vulgaris) is the most important grain legume and plays a significant role in human nutrition being a major source of dietary protein and representing a rich source of minerals and certain vitamins. Several large germplasm collections have been established, which contain large amounts of genetic diversity, including wild and domesticated species. In this study agronomic, biochemical and molecular characterization of landrace bean named "Fagiolo occhio nero di Oliveto Citra" (Phaseolus vulgaris L.), is described. Seeds were obtained by local farmers and field trials were carried out during years 2009-2010, in the typical cultivation site (Oliveto Citra, Salerno Province), using two different densities of investment. During 2011, in order to evaluate the performance in different environments, field trials were conducted in three localities (Battipaglia, Oliveto Citra and Controne). Data analysis shows good adaptability across locations and similar grain yield using two spacing's of seeds. Morphological characterization and molecular analysis, using AFLP and Minisatellite molecular markers, were performed on ten "biotypes" collected from local farmers. Seeds characterization showed variability on the violet area surrounding the hilum (named as eye) while markers have provided useful information on relationships between biotypes. Biochemical analysis, which includes the contents of protein, minerals and antioxidants, shows how the composition is consistent with respect to other landraces and commercial cultivars. The landrace under study revealed genetic stability and good adaptation to cultivated environment with best performance in the native area. In addition, the bio-agronomic characteristics are in accord with studies reported in literature.

  4. Comparison of volatile constituents, and antioxidant and antibacterial activities of the essential oils of Thymus caucasicus, T. kotschyanus and T. vulgaris.

    Science.gov (United States)

    Asbaghian, Shiva; Shafaghat, Ali; Zarea, Khalil; Kasimov, Fakhraddin; Salimi, Farshid

    2011-01-01

    A comparison of the chemical composition, and antioxidant and antibacterial activities of the essential oils obtained from the aerial parts of Thymus caucasicus, T. kotschyanus, and T vulgaris was carried out. The oils, obtained by hydrodistillation, were analyzed by GC and GC/MS. Twenty, 29 and 22 compounds representing 94.8%, 96.6% and 98.2% of the essential oils of T. caucasicus, T. kotschyanus and T. vulgaris, respectively, have been identified. The oil of T. caucasicus was characterized by 1,8-cineol (21.5%), thymol (12.6%), beta-fenchyl alcohol (8.7%), nerolidol (7.8%), terpinolene (7.2%), alpha-pinene (7.0%) and myrcene (6.8%). In the oil of T. kotschyanus, carvacrol (24.4%), beta-caryophyllene (14.5%), gamma-terpinene (12.4%), alpha-phellandrene (10.8%), p-cymene (9.8%) and thymol (6.8%) were the predominant compounds, whereas the main components of T. vulgaris oil were thymol (43.8%), p-cymene (15.2%), germacrene-D (11.7%), terpinolene (3.4%), carvacrol (3.2%), beta-caryophyllene (2.8%) and alpha-thujene (2.2%). In all three plants oil, hydrocarbon monoterpenes predominated over sesquiterpenes. Antioxidant activities were assessed by determining IC50 values in the DPPH radical scavenging assay. Antibacterial activity was determined by measuring minimum inhibitory concentration (MIC) using the broth dilution method. The essential oils of T. caucasicus, T. kotschyanus and T. vulgaris showed free radical scavenging and antibacterial activity.

  5. [Research on characteristic of interrelationship between toxic organic compound BPA and Chlorella vulgaris].

    Science.gov (United States)

    Chen, Shan-Jia; Chen, Xiu-Rong; Yan, Long; Zhao, Jian-Guo; Zhang, Fei; Jiang, Zi-Jian

    2014-04-01

    The effects of different concentrations of bisphenol A (BPA) on Chlorella vulgaris and removal capacity of BPA by Chlorella vulgaris were investigated. Results showed that a low concentration (0-20 mg x L(-1)) of BPA promoted the growth of Chlorella vulgaris, whereas a relative high concentration (20-50 mg x L(-1)) of BPA inhibited the growth of Chlorella vulgaris, and the inhibition effect was positively correlated with the concentration of BPA. Likewise, a high dose of initial BPA (> 20 mg x L(-1)) led to a decline in the content of chlorephyll a. Chlorella vulgaris had BPA removal capacity when initial BPA concentration ranged from 2 mg x L(-1) to 50 mg x L(-1). There was positive correlation between the removal rate of BPA per cell and initial BPA concentration. The removal rate of BPA was the highest when initial BPA was 50 mg x L(-1), which appeared between lag phase and logarithmic phase.

  6. Nitrogen assimilation by nodulate plants of Phaseolus vulgaris l. and Vigna unguiculata (l. ) walp

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    Neves, M.C.P.; Fernandes, M.S.; Sa, M.F.M. (Universidade Federal Rural do Rio de Janeiro (Brazil). Dept. de Solos)

    1982-05-01

    Under field conditions, the processes of nitrogen assimilation via nitrogenase and nitrate-reductase, the transport and the accumulation of nitrogen in nodulated plants of Phaseolus vulgaris cv. Rio Tibagi and Vigna unguiculata cv. Vita 34 were compared and contrasted. V. unguiculata showed better nodulation than P. vulgaris and consequently had higher rates of nitrogenase activity. The small nodulation of P. vulgaris resulted in greater dependence on soil mineral nitrogen as indicated by the higher rates of nitrate-reductase acitivty compared with V. unguiculata, especially during reproductive stage of growth. The superiority of V. unguiculata in terms of assimilation and remobilization of stored nitrogen resulted in a seed yield 28% greater than that of P. vulgaris. P. vulgaris showed a negative correlation between the nitrate-reductase activity and the ureide content of the sap indicating that the metabolic pathways leading to ureide production operates alternatively to nitrate assimilation.

  7. Proof-Carrying Code with Correct Compilers

    Science.gov (United States)

    Appel, Andrew W.

    2009-01-01

    In the late 1990s, proof-carrying code was able to produce machine-checkable safety proofs for machine-language programs even though (1) it was impractical to prove correctness properties of source programs and (2) it was impractical to prove correctness of compilers. But now it is practical to prove some correctness properties of source programs, and it is practical to prove correctness of optimizing compilers. We can produce more expressive proof-carrying code, that can guarantee correctness properties for machine code and not just safety. We will construct program logics for source languages, prove them sound w.r.t. the operational semantics of the input language for a proved-correct compiler, and then use these logics as a basis for proving the soundness of static analyses.

  8. Computer Simulation Instruction: Carrying out Chemical Experiments

    Directory of Open Access Journals (Sweden)

    Ibtesam Al-Mashaqbeh

    2014-05-01

    Full Text Available The purpose of this study was to investigate the effect of computer simulation Instruction (CSI on students' achievements: Carrying out chemical experiments to acquire chemical concepts for eleventh grade students. The subject of the study consisted two sections of a one girl's high school in Jordan. One section was randomly assigned to experimental group in which computer simulation Instruction (CSI was used, and the other section was randomly assigned to control group in which students were instructed by using the traditional teaching instruction. The findings indicated that there is progress on the part of the experimental group which used the computer simulation Instruction (CSI and this was reflected positively in the students’ achievement in carrying out chemical experiments to acquire chemical concepts.

  9. A decimal carry-free adder

    Science.gov (United States)

    Nikmehr, Hooman; Phillips, Braden; Lim, Cheng-Chew

    2005-02-01

    Recently, decimal arithmetic has become attractive in the financial and commercial world including banking, tax calculation, currency conversion, insurance and accounting. Although computers are still carrying out decimal calculation using software libraries and binary floating-point numbers, it is likely that in the near future, all processors will be equipped with units performing decimal operations directly on decimal operands. One critical building block for some complex decimal operations is the decimal carry-free adder. This paper discusses the mathematical framework of the addition, introduces a new signed-digit format for representing decimal numbers and presents an efficient architectural implementation. Delay estimation analysis shows that the adder offers improved performance over earlier designs.

  10. Like an eagle carries its young

    Directory of Open Access Journals (Sweden)

    Hans-Georg Wünch

    2016-04-01

    Full Text Available The picture of an eagle carrying its young on its wings (Dt 32:11 is a powerful and encouraging image of trust and security in God. It is particularly relevant for Western culture, where the eagle is a prominent symbol of power and strength. In recent years, though, the translation of the Hebrew term רֶשֶׁנ as ‘eagle’ has come into question and modern exegetes claim that it is more accurately translated as ‘vulture’. But can this really be a symbol of comfort? Furthermore, do eagles (or vultures even carry their young on their wings? This article intends to shed some light on these questions.Keywords: Old Testament; Deuteronomy; Eagle; Vulture

  11. A Feminist Reading of Sister Carrie

    Institute of Scientific and Technical Information of China (English)

    Wang; Jiatong

    2015-01-01

    In the history of American literature,Sister Carrie is the first novel of Theodore Dreiser,it impresses people deeply.Carrie,a poor country girl,becomes a famous star in a big city.She has totally changed from her hard experiences,and she becomes financially independent as a new woman when she goes through hesitation.In he whole novel,the author has planted some strong points of Carrie’s character.At the end of this paper,it analyzes woman’s status in modern time from two aspects of the improvement of female’s social status and the comparison between men and women.

  12. Potential Health Effects of Enzymatic Protein Hydrolysates from Chlorella vulgaris

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    Mahsa Sedighi

    2016-06-01

    Full Text Available Background and Objective: Chlorella vulgaris is a multi-cellular edible algal species with abundant proteins. Extraction of high value protein fractions for pharmaceutical and nutritional applications can significantly increase the commercial value of microalga biomasses. There is no known report on the anticancer peptides derived from the Chlorella vulgaris abundant protein.Materials and Methods: This study examined the antimicrobial and anticancer effects of peptides from a hydrolyzed Chlorella vulgaris protein with 62 kDa molecular weight. Protein hydrolysis was done by pepsin as a gastrointestinal protease, and was monitored through protein content measurement, sodium dodecyl sulfate polyacrylamide gel electrophoresis, and high performance liquidchromatography measurements. Inhibitory effect of the produced peptides on Escherichia coli cells and breast cancer cell lines was assayed.Results and Conclusion: Hydrolyzed peptides induced a decrease of about 34.1% in the growth of Escherichia coli, and the peptides of 3 to 5 kDa molecular weight had strong impact on the viability of breast cancer cells with IC50 value of 50 μg μl-1. The peptide fractions demonstrating antimicrobial and anti-cancer activities have the potential for use as functional food ingredients for health benefits. These results demonstrate that inexpensive algae proteinscould be a new alternative to produce anticancer peptides.Conflict of interest: The authors declare that there is no conflict of interest.

  13. Efficacy and Safety of Topical Niacinamide for Acne Vulgaris

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    Zeynep Nurhan Saraçoğlu

    2011-06-01

    Full Text Available Background and Design: To investigate the efficacy and safety of topical 4% naicinamide gel cream in the treatment of mild to moderate acne vulgaris and to assess the quality of life of acne patients.Material and Method: Twenty-nine female patients aged 16-38 (mean: 23.57±5.42 years with mild to moderate acne vulgaris who presented in dermatology outpatient clinic were enrolled in the study. All patients applied 4% niacinamide gel cream (Vivatinell-acnecinamide gel cream® on their faces twice daily for eight weeks. The number of lesions (inflammatory and non-inflammatory was counted at 0, 2, 4 and 8 weeks. The side effects (erythema, desquamation, burning and dryness were recorded. The Skindex-29, a quality-of-life measure for patients with skin disease, was administered to the subjects at the beginning and the end of treatment.Results: The decrease in the mean number of inflammatory lesions was statistically significant at the end of the treatment (pre-treatment vs. post-treatment: 12.24 vs. 6.14; p =0.000. However, there was no statistically significant decrease in the number of non-inflammatory lesions at the end of the eight weeks. The niacinamide gel cream was generally well tolerated. There was statistically significant improvement in the Skindex-29 scale scores (p =0.000 at the end of the treatment.Conclusion: Topical 4% niacinamide gel cream may be an alternative treatment for inflammatory lesions of mild to moderate acne vulgaris.

  14. Managing nonteratogenic adverse reactions to isotretinoin treatment for acne vulgaris.

    Science.gov (United States)

    Reilly, Bridget K; Ritsema, Tamara S

    2015-07-01

    Isotretinoin is the strongest, most effective oral treatment for patients with severe acne vulgaris, with remission rates of 89% and higher. Because of its potency, isotretinoin causes many adverse reactions. This article reviews common and severe adverse reactions to isotretinoin and how providers can best manage these reactions. Because of inconclusive research on the correlation between isotretinoin and depression and irritable bowel syndrome, providers should ask patients about symptoms monthly. Prescribing micronized isotretinoin and starting at the lowest dose with gradual upward titration also can help reduce the incidence of adverse reactions.

  15. Monoterpenes from thyme (Thymus vulgaris) as potential mosquito repellents.

    Science.gov (United States)

    Park, Byeoung-Soo; Choi, Won-Sik; Kim, Jeong-Han; Kim, Kap-Ho; Lee, Sung-Eun

    2005-03-01

    Five monoterpenes (carvacrol, p-cymene, linalool, alpha-terpinene, and thymol) derived from the essential oil of thyme (Thymus vulgaris) were examined for their repellency against the mosquito Culex pipiens pallens. All 5 monoterpenes effectively repelled mosquitoes based on a human forearm bioassay. Alpha-terpinene and carvacrol showed significantly greater repellency than a commercial formulation, N,N-diethyl-m-methylbenzamide (deet), whereas thymol showed similar repellency to that of deet. The duration of repellency after application for all these monoterpenes was equal to or higher than that of deet. These findings indicate that a spray-type solution containing 2% alpha-terpinene may serve as an alternative mosquito repellent.

  16. Supercritical CO2 extraction of essential oils from Thymus vulgaris

    Directory of Open Access Journals (Sweden)

    S.A.B. Vieira de Melo

    2000-09-01

    Full Text Available Supercritical CO2 extraction of essential oil from Thymus vulgaris leaves was studied using experimental data recently obtained in the Florys S.p.A. laboratory. Mass transfer coefficients in the supercritical and solid phases from extraction curves at 40°C and 20 MPa were evaluated using a mathematical model based on the local adsorption equilibrium of essential oil on lipid in leaves. The adsorption equilibrium constant was fitted to these experimental data, and internal and external mass transfer resistances were calculated, allowing identification of the mechanism controlling the extraction process.

  17. Lupus vulgaris with tubercular lymphadenitis and IgA nephropathy.

    Science.gov (United States)

    Khaira, Ambar; Rathi, Om P; Mahajan, Sandeep; Sharma, Alok; Dinda, Amit K; Tiwari, Suresh C

    2008-02-01

    A 14-year-old girl presented with a 10-year history of a large crusted plaque over the right thigh for 10 years and small reddish plaque over the left upper back for 3 months. On routine evaluation, she was found to have hematuria. Skin biopsy from the lesion was suggestive of skin tuberculosis (lupus vulgaris), and kidney biopsy showed features of IgA nephropathy (IgAN). Fine-needle aspiration from the inguinal lymph node was consistent with granulomatous disease. The patient has been on anti-tubercular treatment, and the hematuria has subsided.

  18. Ulcerative lupus vulgaris over nose, leading to cosmetic deformity.

    Science.gov (United States)

    Nair, Pragya A; Mehta, Malay J; Patel, Bhumi B

    2015-01-01

    Lupus vulgaris (LV), is a chronic and progressive form of secondary cutaneous tuberculosis. In India, it is commonly seen over buttocks, thighs, and legs whereas involvement of nose is quite rare. Ulcerative variant particularly over nose causes destruction of cartilage, leading to irreversible deformities and contracture. High-index of suspicion is required for early diagnosis and prevention of cosmetic deformity. A case of LV over nose in a young male with ulceration is reported who responded well to anti-tubercular therapy, but left with scarring of nose, which could have been prevented if adequate awareness regarding extra-pulmonary cases would have been practiced.

  19. Azetidine-2-carboxylic acid in garden beets (Beta vulgaris).

    Science.gov (United States)

    Rubenstein, Edward; Zhou, Haihong; Krasinska, Karolina M; Chien, Allis; Becker, Christopher H

    2006-05-01

    Azetidine-2-carboxylic acid (L-Aze) is a toxic and teratogenic non-protein amino acid. In many species, including man, L-Aze is misincorporated into protein in place of proline, altering collagen, keratin, hemoglobin, and protein folding. In animal models of teratogenesis, it causes a wide range of malformations. The role of L-Aze in human disease has been unexplored, probably because the compound has not been associated with foods consumed by humans. Herein we report the presence of L-Aze in the garden or table beet (Beta vulgaris).

  20. Spina ventosa with lupus vulgaris and lymphadenopathy: Multifocal tuberculosis.

    Science.gov (United States)

    Sharma, Sanjeev; Sood, Shikha; Gupta, Mudita

    2015-12-01

    Tuberculous dactylitis is a rare yet well-recognized disease of small bones of the hands and feet. It occurs in young children below five years of age. Tubercular dactylitis with lupus vulgaris and lymphadenopathy was suspected clinically and radiologically in an 8-year-old girl who had multiple soft tissue swelling of hands and feet with ulceration, encrustations, and an atrophic scar with lytic expansile lesions of the small bones of the hands and feet. Tubercular lymph node involvement was confirmed histopathologically.

  1. The Paleobiolinguistics of the Common Bean (Phaseolus vulgaris L.

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    Cecil H. Brown

    2014-10-01

    Full Text Available Paleobiolinguistics is used to determine when and where the common bean (Phaseolus vulgaris L. developed significance for prehistoric groups of Native America. Dates and locations of proto-languages for which common bean terms reconstruct generally accord with crop-origin and dispersal information from plant genetics and archaeobotany. Paleobiolinguistic and other lines of evidence indicate that human interest in the common bean became significant primarily with the widespread development of a village‐farming way of life in the New World rather than earlier when squash and maize and a few other crops became important.

  2. Blanching of green bean (Phaseolus vulgaris).

    Science.gov (United States)

    Kaack, K

    1994-12-01

    Experiments with one and two steps blanching of green beans have been carried out. Inactivation of the peroxydase requires more heating than inactivation of the enzymes which gives rise to off flavour from aldehydes. When blanching for about one minute to inactivate lipoxygenase, aldehyde formation of flavour ceases. The content of vitamin C decreases during blanching according to a first order reaction. Since considerable loss of vitamin C occurs during blanching, the treatment time should be reduced to a minimum. During preblanching at 65-75 degrees C and final blanching, chlorophyll is degraded to pheophytin and the surface colour expressed by the Hunter-values (-a/b) increases with time which means that the colour of the beans changes from green to yellow. The firmness of beans, which was measured by use of a tenderometer, decreases during blanching according to a first order reaction with 40 kcal/mole activation energy. Preblanching at 65-75 degrees C increases the firmness of the beans linearly with treatment time. This increase in firmness is stable after final blanching at 95 degrees C and even after thawing of frozen beans.

  3. Information carrying capacity of a cosmological constant

    Science.gov (United States)

    Simidzija, Petar; Martín-Martínez, Eduardo

    2017-01-01

    We analyze the exchange of information in different cosmological backgrounds when sender and receiver are timelike separated and communicate through massless fields (without the exchange of light signals). Remarkably, we show that the dominance of a cosmological constant makes the amount of recoverable information imprinted in the field by the sender extremely resilient: it does not decay in time or with the spatial separation of the sender and receiver, and it actually increases with the rate of expansion of the Universe. This is in stark contrast with the information carried by conventional light signals and with previous results on timelike communication through massless fields in matter-dominated cosmologies.

  4. Efecto del AIB y el TDZ en el enraizamiento in vitro de plantas de Bambusa vulgaris var vulgaris Schrad. ex Wendl

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    Yudith García Ramírez

    2012-09-01

    Full Text Available Normal 0 21 false false false ES-CO X-NONE X-NONE MicrosoftInternetExplorer4 Título en ingles: Effect of IBA and TDZ on in vitro rooting of plants Bambusa vulgaris var. vulgaris Schrad. ex Wendl. Resumen: Bambusa vulgaris var. vulgaris Schard. ex Wendl. sobresale dentro del género por sus propiedades físico-mecánicas y por el tamaño de sus culmos. Desarrollar la propagación vía organogénesis sería una alternativa para propagar esta especie. Sin embargo, los bajos porcentajes de enraizamiento y de superviviencia ex vitro han sido elementos que han afectado la propagación de diferentes especies de bambúes. Con el objetivo de determinar el efecto de diferentes concentraciones de AIB y TDZ en la emisión de raíces in vitro, se evaluó el número de plantas con raíces, la altura de las plantas (cm desde la base hasta el punto de inserción de la primera hoja y el largo de la raíz (cm. Los resultados demostraron que tanto el AIB como el TDZ influyeron en el enraizamiento in vitro de Bambusa vulgaris var vulgaris. Al adicionar al medio de cultivo TDZ (0,6 mg.L-1 se obtuvieron los mayores porcentajes de formación de raíces (88,2% y estas fueron emitidas mostrando un geotropismo positivo. Palabras clave: bambú; cultivo de tejidos; reguladores de crecimiento. Abstract: Bambusa vulgaris var. vulgaris Schard. ex Wendl. stands out into the genus by its physic - mechanical properties and the culms size. Propagation via organogenesis would be an alternative to propagate this species. However, the low percentages of rooting and ex vitro survival have been elements that have affected the propagation of different bamboo species. The number of plants with roots, plant height (cm (from the base to the insertion point of the first leaf and root length (cm were evaluated in order to determine the effect of different concentrations of IBA and TDZ in the emission of roots in vitro. Results demonstrated that both, AIB and TDZ, influenced the in vitro

  5. Identical MHC markers in non-Jewish Iranian and Ashkenazi Jewish patients with pemphigus vulgaris: possible common central Asian ancestral origin.

    Science.gov (United States)

    Mobini, N; Yunis, E J; Alper, C A; Yunis, J J; Delgado, J C; Yunis, D E; Firooz, A; Dowlati, Y; Bahar, K; Gregersen, P K; Ahmed, A R

    1997-09-15

    Previous studies showed that almost all Ashkenazi Jewish patients with pemphigus vulgaris carried the extended haplotype [HLA-B38, SC21, DRB1*0402, DQB1*0302] or [HLA-B35, SC31, DRB1*0402, DQB1*0302] or class II fragments of them. Non-Jewish patients carried [HLA-B55, SB45, DRB1*1401, DQB1*0503] or its class II fragments. In the present study of 20 Iranian patients with pemphigus vulgaris, 17 were found to carry DRB1*0402, DQB1*0302 haplotypes, also found among normal Iranian haplotypes and the same as that of the Jews. These findings suggest that the pemphigus MHC susceptibility gene among Iranians derived from the same ancestor as that in the Ashkenazim. The ancient Jews were under Persian domination from 500 B.C. until 300 B.C. and in the 8th century A.D., a Tataric people living in the kingdom of Khazar on the Western shore of the Caspian Sea and the Northern shore of the Black Sea, near Persia, converted to Judaism, providing possible opportunities for gene mixing in two populations that are distinct and separate today.

  6. Effects of sodium pentaborate pentahydrate exposure on Chlorella vulgaris growth, chlorophyll content, and enzyme activities.

    Science.gov (United States)

    Chen, Xueqing; Pei, Yuansheng

    2016-10-01

    Sodium pentaborate pentahydrate (SPP) is a rare mineral. In this study, SPP was synthesized from boric acid and borax through low-temperature crystallization, and its effects on the growth of the alga, Chlorella vulgaris (C. vulgaris) were assessed. The newly synthesized SPP was characterized by chemical analysis, X-ray diffraction, Fourier-transform infrared spectroscopy, Raman spectroscopy, thermogravimetric analysis, and differential thermal analysis. The changes in C. vulgaris growth, chlorophyll content, and enzyme activities upon exposure to SPP for 168h were evaluated. Results showed that SPP treatment was detrimental to C. vulgaris growth during the first 24-120h of exposure. The harmful effects, however, diminished over time (168h), even at an effective medium concentration of 226.37mg BL(-1) (the concentration of boron applied per liter of culture medium). A similar trend was observed for chlorophyll content (chlorophyll a and b) and indicated that the photosynthesis of C. vulgaris was not affected and that high levels of SPP may even promote chlorophyll synthesis. Superoxide dismutase and catalase activities of C. vulgaris increased during 24-120h exposure to SPP, but these activities gradually decreased as culture time progressed. In other words, the initial detrimental effects of synthetic SPP on C. vulgaris were temporary and reversible. This research provides a scientific basis for applications of SPP in the environment.

  7. A meta-analysis of association between acne vulgaris and Demodex infestation.

    Science.gov (United States)

    Zhao, Ya-E; Hu, Li; Wu, Li-Ping; Ma, Jun-Xian

    2012-03-01

    Until now, etiology of acne vulgaris is still uncertain. Although clinicians usually deny the association between Demodex infestation and acne vulgaris, it has been proved in some clinical practices. To confirm the association between Demodex infestation and acne vulgaris, a meta-analysis was conducted. Predefined selection criteria were applied to search all published papers that analyzed the association between Demodex infestation and acne vulgaris (January 1950 to August 2011) in ISI Web of Knowledge, MEDLINE, and China National Knowledge Infrastructure (CNKI) databases. A meta-analysis was performed to calculate odds ratios (ORs) and 95% confidence intervals (CIs) based on fixed effects models or random effects models. We enrolled the 60 Chinese and 3 English papers in this meta-analysis, which covered Turkey and 25 different provinces/municipalities in China and 42130 participants including students and residents, aged from 1 to 78 years. The pooled OR in random effects models is 2.80 (95% CI, 2.34-3.36). Stability is robust according to sensitivity analysis. The fail-safe number is 18477, suggesting that at least 18477 articles with negative conclusions would be needed to reverse the conclusion that acne vulgaris was related to Demodex infestation. So the effect of publication bias was insignificant and could be ignored. It was concluded that acne vulgaris is associated with Demodex infestation. This indicates that when regular treatments for acne vulgaris are ineffective, examination of Demodex mites and necessary acaricidal therapies should be considered.

  8. Inhibitory effects of Iranian Thymus vulgaris extracts on in vitro growth of Entamoeba histolytica.

    Science.gov (United States)

    Behnia, Maryam; Haghighi, Ali; Komeylizadeh, Hossein; Tabaei, Seyyed-Javadi Seyyed; Abadi, Alireza

    2008-09-01

    One of the most common drugs used against a wide variety of anaerobic protozoan parasites is metronidazole. However, this drug is mutagenic for bacteria and is a potent carcinogen for rodents. Thymus vulgaris is used for cough suppression and relief of dyspepsia. Also it has antibacterial and antifungal properties. The aim of this study was to investigate antiamebic effect of Thymus vulgaris against Entamoeba histolytica in comparison with metronidazole. One hundred gram air-dried T. vulgaris plant was obtained and macerated at 25 degrees C for 14 days using n-hexane and a mixture of ethanol and water. For essential oil isolation T. vulgaris was subjected to hydrodistillation using a clevenger-type apparatus for 3 hr. E. histolytica, HM-1: IMSS strain was used in all experiments. It was found that the minimal inhibitory concentration (MIC) for T. vulgaris hydroalcoholic, hexanic extracts, and the essential oil after 24 hr was 4 mg/mL, 4 mg/mL, and 0.7 mg/mL, respectively. After 48 hr the MIC for T. vulgaris hydroalcoholic and hexanic extracts was 3 and 3 mg/mL, respectively. Therefore, it can be concluded that the Iranian T. vulgaris is effective against the trophozoites of E. histolytica.

  9. Inhibitory effect ofThymus vulgaris extract on memory impairment induced by scopolamine in rat

    Institute of Scientific and Technical Information of China (English)

    Zahra Rabiei; Shiva Mokhtari; Samira Asgharzade; Mostafa Gholami; Samira Rahnama; Mahmoud Rafieian-kopaei

    2015-01-01

    Objective:To investigate the effect ofThymus vulgaris (T. vulgaris) on learning and memory functions in scopolamine-induced memory deficit in rats. Memory enhancing activity in scopolamine-induced amnesic rats was investigated by assessing the Morris water maze and passive avoidance paradigm. Methods:A total of 42 male Wistar rats were divided into 6 equal groups as follow: control group: received water, scopolamine treated group: received scopolamine 1 mg/kg for 15 days, two scopolamine+T. vulgaris treated groups: received scopolamine andT. vulgaris extract 50 and 100 mg/kg body weight per day for 15 days, two intact groups: receivedT. vulgaris extract 50 and 100 mg/kg body weight per day for 15 days. Results: Administration ofT. vulgaris extract significantly restored memory and learning impairments induced by scopolamine in the passive avoidance test and Morris water maze test. Conclusions:T. vulgaris extract has repairing effects on memory and behavioral disorders produced by scopolamine and may have beneficial effects in the treatment of Alzheimer's disease.

  10. Inhibitory effect of Thymus vulgaris extract on memory impairment induced by scopolamine in rat简

    Institute of Scientific and Technical Information of China (English)

    Zahra; Rabiei; Shiva; Mokhtari; Samira; Asgharzade; Mostafa; Gholami; Samira; Rahnama; Mahmoud; Rafieian-kopaei

    2015-01-01

    Objective: To investigate the effect of Thymus vulgaris(T. vulgaris) on learning and memory functions in scopolamine-induced memory deficit in rats. Memory enhancing activity in scopolamine-induced amnesic rats was investigated by assessing the Morris water maze and passive avoidance paradigm.Methods: A total of 42 male Wistar rats were divided into 6 equal groups as follow:control group: received water, scopolamine treated group: received scopolamine 1 mg/kg for 15 days, two scopolamine + T. vulgaris treated groups: received scopolamine and T. vulgaris extract 50 and 100 mg/kg body weight per day for 15 days, two intact groups:received T. vulgaris extract 50 and 100 mg/kg body weight per day for 15 days.Results: Administration of T. vulgaris extract significantly restored memory and learning impairments induced by scopolamine in the passive avoidance test and Morris water maze test.Conclusions: T. vulgaris extract has repairing effects on memory and behavioral disorders produced by scopolamine and may have beneficial effects in the treatment of Alzheimer’s disease.

  11. [Physiological responses of sugar beet (Beta vulgaris) to drought stress during vegetative development period under drip irrigation].

    Science.gov (United States)

    Li, Yang-yang; Geng, Qing-yun; Fei, Cong; Fan, Huai

    2016-01-01

    Sugar beet (Beta vulgaris cv. Beta 356) was subjected to drought stress during vegetative development by maintaining the soil water content in the 0-40 cm soil depth at 70%, 50% or 30% of field capacity to study the physiological traits of the leaves. Results showed that the compensation index was the highest in the 50% field capacity treatment. Malonaldehyde (MDA) content, relative conductivity, catalase (CAT) activity, and soluble sugar content began to increase 24 h after rehydration. Proline content began to increase 48 h after rehydration. In contrast, no compensation effect was observed in peroxidase (POD) activity after rehydration. Among the active oxygen scavenging enzymes, CAT was most sensitive to drought stress. Supplemental irrigation should be carried out promptly when the soil water content dropped to 50% of field capacity during vegetative development. Rehydration could promote self-repair functions in leaves, thus reducing the effects of drought on sugar beet yield and sugar content.

  12. In Vitro and In Vivo Anti-inflammatory Effects of Struthanthus vulgaris.

    Science.gov (United States)

    Marques, Franciane Martins; da Costa, Maycow Rodrigues; Vittorazzi, Cátia; Gramma, Luciane De Souza Dos Santos; Barth, Thiago; de Andrade, Tadeu Uggere; Endringer, Denise Coutinho; Scherer, Rodrigo; Fronza, Marcio

    2017-06-01

    Struthanthus vulgaris is probably the most common medicinal mistletoe plant in Brazil, and has been used in folk medicine as an anti-inflammatory agent and for cleaning skin wounds. Our proposal was to evaluate the anti-inflammatory activity of S. vulgaris ethanol leaf extract and provide further insights of how this biological action could be explained using in vitro and in vivo assays. In vitro anti-inflammatory activity was preliminarily investigated in lipopolysaccharide/interferon gamma-stimulated macrophages based on their ability to inhibit nitric oxide production and tumor necrosis factor-alpha. In vivo anti-inflammatory activity of S. vulgaris ethanol leaf extract was investigated in the mice carrageenan-induced inflammation air pouch model. The air pouches were inoculated with carrageenan and then treated with 50 and 100 mg/kg of S. vulgaris ethanol leaf extract or 1 mg/kg of dexamethasone. Effects on the immune cell infiltrates, pro- and anti-inflammatory mediators such as tumor necrosis factor-alpha, interleukin 1, interleukin 10, and nitric oxide, were evaluated. The chemical composition of S. vulgaris ethanol leaf extract was characterized by LC-MS/MS. In vitro S. vulgaris ethanol leaf extract significantly decreased the production of nitric oxide and tumor necrosis factor-alpha in macrophages and did not reveal any cytotoxicity. In vivo, S. vulgaris ethanol leaf extract significantly suppressed the influx of leukocytes, mainly neutrophils, protein exudation, nitric oxide, tumor necrosis factor-alpha, and interleukin 1 concentrations in the carrageenan-induced inflammation air pouch. In conclusion, S. vulgaris ethanol leaf extract exhibited prominent anti-inflammatory effects, thereby endorsing its usefulness as a medicinal therapy against inflammatory diseases, and suggesting that S. vulgaris ethanol leaf extract may be a source for the discovery of novel anti-inflammatory agents. Georg Thieme Verlag KG Stuttgart · New York.

  13. Quality of life, self-esteem and psychosocial factors in adolescents with acne vulgaris*

    Science.gov (United States)

    Vilar, Gustavo Nunes; dos Santos, Laís Araújo; Sobral Filho, Jader Freire

    2015-01-01

    Background Dermatological diseases, among which acne vulgaris, have psychological impact on the affected generating feelings of guilt, shame and social isolation. Objectives To compare quality of life, self-esteem and other psychosocial variables amongst adolescents with and without acne vulgaris, and between levels of severity. Methods Cross-sectional observational study in a sample of 355 high school students from the city of João Pessoa. Data collection was performed with questionnaires and clinical-dermatological evaluation. The primary variables were the incidence of AV; quality of life, set by the Children's Dermatology Quality of Life Index and Dermatology Quality of Life Index; and self-esteem, measured by the Rosenberg Self-Esteem Scale. For calculation of statistical tests, we used the SPSS 20.0 software, considering p=0.05. Results The sample, with an average age of 16, showed 89.3% prevalence of acne vulgaris. The most prevalent psychosocial issue was "afraid that acne will never cease", present in 58% of affected youth. The median score of Quality of Life in Children's Dermatology Index was different amongst students with and without acne vulgaris (p=0.003), as well as the Quality of Life in Dermatology (p=0.038) scores, so that students with acne vulgaris have worse QoL. There was a correlation between the severity of acne vulgaris and worse quality of life. Self-esteem was not significantly associated with the occurrence or severity of acne vulgaris. Conclusions acne vulgaris assumes significance in view of its high prevalence and the effect on quality of life of adolescents, more severe at the more pronounced stages of disease (p<0.001). The psychosocial impact of acne vulgaris should be valued in the management of patients with this condition. PMID:26560206

  14. Quality of life, self-esteem and psychosocial factors in adolescents with acne vulgaris.

    Science.gov (United States)

    Vilar, Gustavo Nunes; Santos, Laís Araújo dos; Sobral Filho, Jader Freire

    2015-01-01

    Dermatological diseases, among which acne vulgaris, have psychological impact on the affected generating feelings of guilt, shame and social isolation. To compare quality of life, self-esteem and other psychosocial variables amongst adolescents with and without acne vulgaris, and between levels of severity. Cross-sectional observational study in a sample of 355 high school students from the city of João Pessoa. Data collection was performed with questionnaires and clinical-dermatological evaluation. The primary variables were the incidence of AV; quality of life, set by the Children's Dermatology Quality of Life Index and Dermatology Quality of Life Index; and self-esteem, measured by the Rosenberg Self-Esteem Scale. For calculation of statistical tests, we used the SPSS 20.0 software, considering p=0.05. The sample, with an average age of 16, showed 89.3% prevalence of acne vulgaris. The most prevalent psychosocial issue was "afraid that acne will never cease", present in 58% of affected youth. The median score of Quality of Life in Children's Dermatology Index was different amongst students with and without acne vulgaris (p=0.003), as well as the Quality of Life in Dermatology (p=0.038) scores, so that students with acne vulgaris have worse QoL. There was a correlation between the severity of acne vulgaris and worse quality of life. Self-esteem was not significantly associated with the occurrence or severity of acne vulgaris. acne vulgaris assumes significance in view of its high prevalence and the effect on quality of life of adolescents, more severe at the more pronounced stages of disease (p<0.001). The psychosocial impact of acne vulgaris should be valued in the management of patients with this condition.

  15. The electron transfer system of syntrophically grown Desulfovibrio vulgaris

    Energy Technology Data Exchange (ETDEWEB)

    Walker, C.B.; He, Z.; Yang, Z.K.; Ringbauer, Jr., J.A.; He, Q.; Zhou, J.; Voordouw, G.; Wall, J.D.; Arkin, A.P.; Hazen, T.C.; Stolyar, S.; Stahl, D.A.

    2009-05-01

    Interspecies hydrogen transfer between organisms producing and consuming hydrogen promotes the decomposition of organic matter in most anoxic environments. Although syntrophic couplings between hydrogen producers and consumers are a major feature of the carbon cycle, mechanisms for energy recovery at the extremely low free energies of reactions typical of these anaerobic communities have not been established. In this study, comparative transcriptional analysis of a model sulfate-reducing microbe, Desulfovibrio vulgaris Hildenborough, suggested the use of alternative electron transfer systems dependent upon growth modality. During syntrophic growth on lactate with a hydrogenotrophic methanogen, D. vulgaris up-regulated numerous genes involved in electron transfer and energy generation when compared with sulfate-limited monocultures. In particular, genes coding for the putative membrane-bound Coo hydrogenase, two periplasmic hydrogenases (Hyd and Hyn) and the well-characterized high-molecular weight cytochrome (Hmc) were among the most highly expressed and up-regulated. Additionally, a predicted operon coding for genes involved in lactate transport and oxidation exhibited up-regulation, further suggesting an alternative pathway for electrons derived from lactate oxidation during syntrophic growth. Mutations in a subset of genes coding for Coo, Hmc, Hyd and Hyn impaired or severely limited syntrophic growth but had little affect on growth via sulfate-respiration. These results demonstrate that syntrophic growth and sulfate-respiration use largely independent energy generation pathways and imply that understanding of microbial processes sustaining nutrient cycling must consider lifestyles not captured in pure culture.

  16. The Electron Transfer System of Syntrophically Grown Desulfovibrio vulgaris

    Energy Technology Data Exchange (ETDEWEB)

    PBD; ENIGMA; GTL; VIMSS; Walker, Christopher B.; He, Zhili; Yang, Zamin K.; Ringbauer Jr., Joseph A.; He, Qiang; Zhou, Jizhong; Voordouw, Gerrit; Wall, Judy D.; Arkin, Adam P.; Hazen, Terry C.; Stolyar, Sergey; Stahl, David A.

    2009-06-22

    Interspecies hydrogen transfer between organisms producing and consuming hydrogen promotes the decomposition of organic matter in most anoxic environments. Although syntrophic couplings between hydrogen producers and consumers are a major feature of the carbon cycle, mechanisms for energy recovery at the extremely low free energies of reactions typical of these anaerobic communities have not been established. In this study, comparative transcriptional analysis of a model sulfate-reducing microbe, Desulfovibrio vulgaris Hildenborough, suggested the use of alternative electron transfer systems dependent upon growth modality. During syntrophic growth on lactate with a hydrogenotrophic methanogen, D. vulgaris up-regulated numerous genes involved in electron transfer and energy generation when compared with sulfate-limited monocultures. In particular, genes coding for the putative membrane-bound Coo hydrogenase, two periplasmic hydrogenases (Hyd and Hyn) and the well-characterized high-molecular weight cytochrome (Hmc) were among the most highly expressed and up-regulated. Additionally, a predicted operon coding for genes involved in lactate transport and oxidation exhibited up-regulation, further suggesting an alternative pathway for electrons derived from lactate oxidation during syntrophic growth. Mutations in a subset of genes coding for Coo, Hmc, Hyd and Hyn impaired or severely limited syntrophic growth but had little affect on growth via sulfate-respiration. These results demonstrate that syntrophic growth and sulfate-respiration use largely independent energy generation pathways and imply that understanding of microbial processes sustaining nutrient cycling must consider lifestyles not captured in pure culture.

  17. Recently approved systemic therapies for acne vulgaris and rosacea.

    Science.gov (United States)

    Del Rosso, James Q

    2007-08-01

    Until recently, with the exception of oral isotretinoin for the treatment of severe recalcitrant nodular acne, systemic therapy for acne vulgaris and rosacea has been based on anecdotal support, clinical experience, and small clinical trials. Tetracycline derivatives are the predominant systemic agents that have been used for both disease states, prescribed in dose ranges that produce antibiotic activity. Anti-inflammatory dose doxycycline, a controlled-release (CR) 40-mg capsule formulation of doxycycline that is devoid of antibiotic activity when administered once daily, was US Food and Drug Administration (FDA)-approved for the treatment of inflammatory lesions (papules and pustules) of rosacea, based on large-scale phase 3 pivotal trials and long-term microbiologic and safety data. Also, an extended-release (ER) tablet formulation of minocycline was approved by the FDA for the treatment of inflammatory lesions of moderate to severe acne vulgaris in patients 12 years and older based on large-scale phase 3 clinical trials that evaluated efficacy and safety, dose-response analysis, and long-term data. This article discusses the studies and clinical applications related to the use of these agents.

  18. Measurement of bone mineral density in patients with psoriasis vulgaris

    Directory of Open Access Journals (Sweden)

    Nurgül Köse

    2013-12-01

    Full Text Available Objective: The aim of this study was to determine thechanges of bone mineral density in patients with psoriasisvulgaris.Methods: 33 patients, diagnosed with psoriasis vulgarisin Erciyes University, Medical School, Department of Dermatology,and 23 healthy subjects as control group wereincluded in this study. Lumbar and femur bone mineraldensity of all patients and control group were measured.Biochemical tests related to bone metabolism were performed.Results: We did not find statistically significant differencebetween patient and control groups’ bone mineral density.However, the lumbar spine t score of men, were found tobe significantly lower in the patient group than the controlgroup (p<0.05. There was no statistically significant differencein bone mineral density of women between thepatient and control groups. The effects on bone mineraldensity of disease period and using the topical corticosteroidswere not statistically significant.Conclusion: Our study indicates that bone mineral densityof patients with psoriasis vulgaris was not differentfrom healthy subject.Key words: Psoriasis vulgaris, bone mineral density, osteoporosis

  19. Antimicrobial susceptibility of Propionibacterium acnes isolated from acne vulgaris.

    Science.gov (United States)

    Kurokawa, I; Nishijima, S; Kawabata, S

    1999-01-01

    Systemic and topical antimicrobial treatment for acne vulgaris remains the mainstay method of therapy in Japan. Strains of Propionibacterium acnes (P. acnes) resistant to erythromycin (EM), clindamycin (CLDM), tetracycline (TC), doxycycline (DOXY) and minocycline (MINO) have been reported. The aim of the present study was to examine the antimicrobial susceptibility to 10 currently used antimicrobial agents of 50 strains of P. acnes isolated from acne lesions and identified using a Rap ID ANA II panel. Minimum inhibitory concentrations (MIC) were determined by the agar dilution method according to the criteria of the Japan Society of Chemotherapy. EM, ampicillin (ABPC), and CLDM were the most potent drugs, followed by MINO, nadifloxacin (NDFX), cephalexin (CEX), DOXY, ofloxacin (OFLX), and TC. In terms of the MIC80, EM and ABPC were the most potent, followed by CLDM, NDFX, MINO, CEX, DOXY, OFLX, TC and gentamycin (GM). Although most of the strains used were susceptible to the antimicrobial agents tested, strains of P. acnes resistant (MIC 12.5 mug/ml) to EM (4%), CLDM (4%), DOXY (2%) and TC (2%) were observed. In this study, no strains of P. acnes resistant to MINO were seen, suggesting that oral MINO is the most useful treatment for acne vulgaris with minimal risk of bacterial resistance.

  20. Heteroplasmy and atrazine resistance in Chenopodium album and Senecio vulgaris.

    Science.gov (United States)

    Bühler, Michaela; Bogenrieder, Arno; Sandermann, Heinrich; Ernst, Dieter

    Atrazine-resistant weeds are well known, and the resistance is primarily caused by a point mutation in the psbA chloroplast gene encoding the photosystem II D1 protein. Heteroplasmy, the presence of different types of chloroplasts in an individual plant, is also very common. Thus, atrazine-resistant weeds may also partly possess the atrazine-binding sequence and vice versa. The region of the psbA gene containing the mutation was sequenced from atrazine-resistant and atrazine-sensitive Chenopodium album and Senecio vulgaris plants. In atrazine-sensitive C. album plants, the expected AGT triplet was found. The atrazine-resistant plants contained the expected base substitution (AGT to GGT); however, in addition the AGT triplet was found. The atrazine-resistant S. vulgaris plants contained the expected GGT sequence, whereas the atrazine-sensitive plants contained both the AGT and GGT sequences. This clearly indicates that in addition to Gly264 also Ser264 is present in atrazine-resistant plants, and vice versa in atrazine-sensitive plants, indicating heteroplasmy in these weeds.

  1. Stability and loading properties of curcumin encapsulated in Chlorella vulgaris.

    Science.gov (United States)

    Jafari, Yaser; Sabahi, Hossein; Rahaie, Mahdi

    2016-11-15

    Curcumin (Cur), a polyphenols with pharmacological function, was successfully encapsulated in algae (Alg) cell (Chlorella vulgaris) as confirmed by fluorescence microscopy, thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and Fourier transform-infrared spectroscopy (FT-IR). Fluorescence micrographs, TGA, DSC and FTIR spectra suggested the hypothesis inclusion Cur in Nano-empty spaces inside cell wall of Alg. The TGA analysis showed that the thermal stability of Alg and Cur at algae/curcumin complex was 3.8% and 33% higher than their free forms at 0-300°C and 300-600°C ranges, respectively. After encapsulation in Alg cells, the photostability of Cur was enhanced by about 2.5-fold. Adsorption isotherm of Cur into Alg was fitted with the Freundlich isotherm. The microcapsules were loaded with Cur up to about 55% w/w which is much higher than other reported bio-carriers. In conclusion, the data proved that Chlorella vulgaris cell can be used as a new stable carrier for Cur.

  2. Hubungan Konsumsi Makanan Cepat Saji Terhadap Kejadian Akne Vulgaris pada Mahasiswa FK USU Stambuk 2007

    OpenAIRE

    Bancin, Berry Eka Parda

    2011-01-01

    Introduction. Acne vulgaris is a very common disease found in public, especially in adolescence. About 85% of people in their life have had acne vulgaris, so it’s often assumed as a physiological state. High-calorie foods have been long suspected as one of the causes of this disease, this opinion is supported by the increased incidence of acne vulgaris in developing countries today. Since 1946 to 2007 various studies have been conducted on the relationship of food to the occurrence of acne vu...

  3. Terms referring to psoriasis vulgaris in the classical Chinese medicine literature: a systematic analysis.

    Science.gov (United States)

    Zhang, Claire Shuiqing; May, Brian; Yan, Yuhong; Yu, Jason Jingjie; Yao, Danni; Chang, Suyueh; Zhang, Anthony Lin; Guo, Xinfeng; Lu, Chuanjian; Xue, Charlie Changli

    2016-04-01

    Psoriasis vulgaris is a modern medical term. In the classical Chinese medicine (CM) literature, this disorder could be classified under a diversity of terms. In order to explore how psoriasis vulgaris was conceptualized and managed throughout Chinese medical history, we undertook a systematic longitudinal analysis of descriptions in the classical CM literature of skin disorders consistent with psoriasis vulgaris. Candidate search terms were identified from 33 contemporary CM books as relevant to psoriasis vulgaris. Thirteen terms were used to search the Zhong Hua Yi Dian--'Encyclopaedia of Traditional Chinese Medicine', 4th edition, a digital collection of over 1000 medical books. Search results were entered in a spreadsheet, citations that were consistent with psoriasis vulgaris were identified based on: (i) a description of the features of psoriasis vulgaris and (ii) judgment by two clinicians regarding relevance to psoriasis vulgaris. Analysis focused on the use of terms by historical period (Chinese dynasty) and whether the terms were specific for psoriasis vulgaris 608 citations dating from 363 to 1947AD were included. The two criteria for determining inclusion of citations were combined to select the pool of citations most relevant to psoriasis vulgaris. Sixty citations from eight search terms were found in this pool. Among the eight terms, Bai Bi, She Shi and Bi Feng consistently identified citations that were likely to be psoriasis. The earliest was from 1368AD (Ming dynasty). The remaining five terms yielded inconsistent results. Citations of disorders consistent with psoriasis vulgaris appear prior to 1368-1644AD (Ming dynasty), but the terms used were not specific for psoriasis. Bai Bi, She Shi and Bi Feng are the terms most consistently used to refer to psoriasis vulgaris. They first appeared in 1368-1644AD (Ming dynasty) and Bai bi remains in use. Regarding the other terms, certain citations may have referred to psoriasis vulgaris, but in other cases

  4. Changes in hemostasis in foals naturally infected with Strongylus vulgaris

    DEFF Research Database (Denmark)

    Pihl, Tina Holberg; Krarup Nielsen, Martin; Jacobsen, Stine

    2017-01-01

    enzyme-linked immunosorbent assay values in all foals using a linear mixed effects model with repeated measures and to total numbers of S. vulgaris larvae in nine foals at necropsy with Pearson linear correlation. In the first week of life, all evaluated indices of hemostasis were significantly different...... 4. Strongylus vulgaris antibody levels were statistically associated with D-dimer (P = .0076) and fibrinogen (P = .0004) concentrations. Naturally acquired infection with S. vulgaris was associated with changes suggestive of mild activation of coagulation, fibrinolysis, and inflammation. The results...

  5. Sorption Capacity Measurement of Chlorella Vulgaris and Scenedesmus Acutus to Remove Chromium from Tannery Waste Water

    Science.gov (United States)

    Ardila, Liliana; Godoy, Rubén; Montenegro, Luis

    2017-08-01

    Tanning process is a polluting activity due to the release of toxic agents into the environment. One of the most important of those toxic chemicals is chromium. Different alternatives have been proposed for the removal of this metal from tanning waste water which include the optimization of the productive processes, physicochemical and biochemical waste water treatment. In this study, the biological adsorption process of trivalent chromium was carried out in synthetic water and tannery waste water through two types of native green microalgae, called Chlorella vulgaris and Scenedesmus acutus in Free State and immobilized in PVA state. This, considering that cellular wall of microalgae has functional groups like amines and carboxyl that might bind with trivalent chromium. Statistical significance of variables as pH temperature, chromium and algae concentrations was evaluated just like bio sorption capacity of different types of water and kind of bioadsorbent was calculated to determine if this process is a competitive solution comparing to other heavy metal removal processes.

  6. Weed Interference Effects on Leaves, Internode and Harvest Index of Dry Bean (Phaseolus vulgaris L.

    Directory of Open Access Journals (Sweden)

    Hossein GHAMARI

    2015-03-01

    Full Text Available The development of appropriate weed management strategies and efficient use of herbicides relies upon understanding weed-crop interactions. A field study was carried out to assess the effect of weed interference on leaves, internode and harvest index of dry bean (Phaseolus vulgaris L.. The experiment was established under a randomized complete block design with two types of weed interference treatments: plots with weeds and plots without weeds at different time intervals (0, 10, 20, 30, 40 and 50 days after crop emergence. The sigmoid Boltzmann model was used to quantify the crop traits as influenced by weed interference. Prolonged delays in weed removal reduced gradually the number of leaves of the crop. Weed interference decreased dry weight of leaves as well, so that the lowest value of it (33.49 g plant-1 was observed in full season during weed-infested treatment. Infestation of weeds affected the length of the crop internodes. While the weed interference duration increased, the length of the internodes decreased. Harvest index was also sensitive to weed competition. As the crop was kept weed-infested from the emergence for increasing periods of time, harvest index decreased to a value of 28.01%. A significant negative correlation between total biomass of weeds and dry bean traits (number of leaves, leaves dry weight, internode length and harvest index was observed. Therefore, weeds are able to adversely affect dry bean growth through constraining environmental resources and impairing leaves as the photosynthetic areas.

  7. Redox states of Desulfovibrio vulgaris DsrC, a key protein in dissimilatory sulfite reduction

    Energy Technology Data Exchange (ETDEWEB)

    Venceslau, Sofia S. [Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Oeiras (Portugal); Cort, John R.; Baker, Erin S. [Fundamental and Computational Sciences Directorate, Pacific Northwest National Laboratory, Richland, WA 99352 (United States); Chu, Rosalie K.; Robinson, Errol W. [Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA 99352 (United States); Dahl, Christiane [Institut für Mikrobiologie and Biotechnologie, Rheinische Friedrich-Wilhelms-Universität Bonn, Meckenheimer Allee 168, D-53115 Bonn (Germany); Saraiva, Lígia M. [Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Oeiras (Portugal); Pereira, Inês A.C., E-mail: ipereira@itqb.unl.pt [Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Oeiras (Portugal)

    2013-11-29

    Highlights: •DsrC is known to interact with the dissimilatory sulfite reductase enzyme (DsrAB). •We show that, however, most cellular DsrC is not associated with DsrAB. •A gel-shift assay was developed that allows monitoring of the DsrC redox state. •The DsrC intramolecularly oxidized state could only be produced by arginine treatment. -- Abstract: Dissimilatory reduction of sulfite is carried out by the siroheme enzyme DsrAB, with the involvement of the protein DsrC, which has two conserved redox-active cysteines. DsrC was initially believed to be a third subunit of DsrAB. Here, we report a study of the distribution of DsrC in cell extracts to show that, in the model sulfate reducer Desulfovibrio vulgaris, the majority of DsrC is not associated with DsrAB and is thus free to interact with other proteins. In addition, we developed a cysteine-labelling gel-shift assay to monitor the DsrC redox state and behaviour, and procedures to produce the different redox forms. The oxidized state of DsrC with an intramolecular disulfide bond, which is proposed to be a key metabolic intermediate, could be successfully produced for the first time by treatment with arginine.

  8. PSP toxins profile in ascidian Microcosmus vulgaris (Heller, 1877) after human poisoning in Croatia (Adriatic Sea).

    Science.gov (United States)

    Roje-Busatto, Romana; Ujević, Ivana

    2014-03-01

    Toxins known to cause Paralytic Shellfish Poisoning (PSP) syndrome in humans that can have serious economic consequences for aquaculture were determined in ascidians of the genus Microcosmus. Significant concentrations of toxins were confirmed in all tested samples collected from the western coast of Istria Peninsula (Adriatic Sea, Croatia) when six people were poisoned following the consumption of fresh ascidians. Several species of bivalves that were under continuous monitoring had not accumulated PSP toxins although they were exposed to the same environmental conditions over the survey period. In the present study, HPLC-FLD with pre-column oxidation of PSP toxins has been carried out to provide evidence for the first human intoxication due to consumption of PSP toxic ascidians (Microcosmus vulgaris, Heller, 1877) harvested from the Adriatic Sea. Qualitative analysis established the presence of six PSP toxins: saxitoxin (STX), decarbamoylsaxitoxin (dcSTX), gonyautoxins 2 and 3 (GTX2,3), decarbamoylgonyautoxins 2 and 3 (dcGTX2,3), gonyautoxin 5 (GTX5) and N-sulfocarbamoylgonyautoxins 1 and 2 (C1,2), while quantitative analysis suggested STX and GTX2,3 as dominant toxin types and the ones that contribute the most to the overall toxicity of these samples with concentrations near the regulatory limit.

  9. Study on mutation induced effect of gamma ray and DES on black bean phaseolus vulgaris

    Energy Technology Data Exchange (ETDEWEB)

    Le Thi Dinh; Pham Le Ha; Nguyen Van Toan; Le Xuan Tham [Nuclear Research Institute, Radiobiology Department, Dalat (Viet Nam)

    2001-03-01

    The study on mutation induced effect of gamma ray and DES on black bean Phaseolus vulgaris was carried out at Radiobiology Department, Nuclear Research Institute of Dalat. Dry seeds of variety No.1847 - Bonita - Cuba in set of 13 black bean varieties were irradiated with gamma ray from {sup 60}Co source at dose range from 150 Gy to 350 Gy and treated with DES at concentration from 0.1% to 0.3% in 2 hours for experiments in laboratory. The doses of 200, 250, 300 Gy and concentration of 0.2% DES in 2 hours were selected to treat dry seeds for experiments on the field. In populations of M{sub 1} generation, the height, number of branches and fruits per plant, number of seeds per fruit were decreased with increasing of irradiation doses. In populations of M{sub 2} generation, individual variants in leaf shape, chlorophyll, short stem, dwarf, early maturity, flowering in very short time were obtained and selected in all treatment cases. Mutation frequency at dose of 300 Gy was higher than that in other treatment cases, but ratio of sterility is also largest. The mutant lines of early maturity and short stem with flowering in very short time are promised materials for further studies. (author)

  10. Perbandingan Konsumsi Lemak Berdasarkan Tingkat Keparahan Akne Vulgaris pada Siswa SMK Negeri 1 Kota Jambi

    Directory of Open Access Journals (Sweden)

    Nisa Sulistia

    2015-09-01

    Full Text Available Abstrak Salah satu faktor pencetus akne vulgaris adalah diet tinggi lemak, terutama lemak jenuh. Tujuan penelitian ini adalah untuk mengetahui perbandingan konsumsi lemak berdasarkan tingkat keparahan akne vulgaris menurut kriteria Lehmann. Penelitian ini berupa studi analitik observasional dengan desain cross sectional melalui pemeriksaan statusdermatologikus menurut kriteria Lehmann dan pengambilan data konsumsi menggunakan FFQ (Food Frequency Questionnaire pada 138 siswa kelas X dan XI SMK Negeri 1 Kota Jambi. Hasil penelitian menunjukkan terdapat akne vulgaris derajat ringan (49%, akne vulgaris derajat sedang (42%, dan akne vulgaris derajat berat (9%. Reratakonsumsi lemak total 89,35 ± 17,63 gram. Rerata konsumsi SFA (Saturated Fatty Acid 37,07 ± 9,97 gram. Rerata konsumsi MUFA (Monounsaturated Fatty Acid 15,30 ± 11,79 gram. Rerata konsumsi PUFA (Polyunsaturated Fatty Acid 12,03 ± 9,25 gram.Uji oneway Anova menunjukkan tidak ada perbedaan bermakna rerata konsumsi lemak total (p > 0,05 dan SFA (p > 0,05 berdasarkan tingkat keparahan akne vulgaris. Uji Kruskal-Wallis menunjukkan tidak ada perbedaan bermakna MUFA (p > 0,05, dan PUFA (p > 0,05 berdasarkan tingkat keparahan akne vulgaris. Penelitian ini menunjukkan tidak ada perbedaan konsumsi lemak berdasarkan tingkat keparahan akne vulgaris pada siswa SMKNegeri 1 Kota Jambi.Kata kunci: akne vulgaris, FFQ, lemak total, SFA, MUFA, PUFA Abstract One of the precipited factors is high fat diet, especially saturated fats. The objective of this study was to determine the comparison of fat consumption which is based on the severity of acne vulgaris.This research wasanalytic observational study using cross sectional design, by examining dermatologic status according to Lehmann criteria and  taking consumption record with FFQ (Food Frequency Questionnaire of  138 tenth and eleventh grade student in SMKN 1 Jambi. The result showed that mild acne vulgaris (49%, moderate acne vulgaris (42%, and

  11. DESIGN OF OPTIMAL CARRY SKIP ADDER AND CARRY SKIP BCD ADDER USING REVERSIBLE LOGIC GATES

    OpenAIRE

    Praveena Murugesan; Thanushkodi Keppanagounder

    2014-01-01

    Reversible logic circuits have the ability to produce zero power dissipation which has found its importance in quantum computing, optical computing and low power digital circuits. The study presents improved and efficient reversible logic circuits for carry skip adder and carry skip BCD adder. The performance of the proposed architecture is better than the existing works in terms of gate count, garbage outputs and constant inputs. This design forms the basis for different quantum ALU and embe...

  12. Genetic mapping of two genes conferring resistance to powdery mildew in common bean (Phaseolus vulgaris L.).

    Science.gov (United States)

    Pérez-Vega, Elena; Trabanco, Noemí; Campa, Ana; Ferreira, Juan José

    2013-06-01

    Powdery mildew (PM) is a serious disease in many legume species, including the common bean (Phaseolus vulgaris L.). This study investigated the genetic control behind resistance reaction to PM in the bean genotype, Cornell 49242. The results revealed evidence supporting a qualitative mode of inheritance for resistance and the involvement of two independent genes in the resistance reaction. The location of these resistance genes was investigated in a linkage genetic map developed for the XC RIL population. Contingency tests revealed significant associations for 28 loci out of a total of 329 mapped loci. Fifteen were isolated or formed groups with less than two loci. The thirteen remaining loci were located at three regions in linkage groups Pv04, Pv09, and Pv11. The involvement of Pv09 was discarded due to the observed segregation in the subpopulation obtained from the Xana genotype for the loci located in this region. In contrast, the two subpopulations obtained from the Xana genotype for the BM161 locus, linked to the Co-3/9 anthracnose resistance gene (Pv04), and from the Xana genotype for the SCAReoli locus, linked to the Co-2 anthracnose resistance gene (Pv11), exhibited monogenic segregations, suggesting that both regions were involved in the genetic control of resistance. A genetic dissection was carried out to verify the involvement of both regions in the reaction to PM. Two resistant recombinant lines were selected, according to their genotypes, for the block of loci included in the Co-2 and Co-3/9 regions, and they were crossed with the susceptible parent, Xana. Linkage analysis in the respective F2 populations supported the hypothesis that a dominant gene (Pm1) was located in the linkage group Pv11 and another gene (Pm2) was located in the linkage group Pv04. This is the first report showing the localization of resistance genes against powdery mildew in Phaseolus vulgaris and the results offer the opportunity to increase the efficiency of breeding

  13. Shelf-life extension of minimally processed and gamma irradiated red beet (Beta vulgaris ssp. vulgaris L.), Cv. early wonder

    Energy Technology Data Exchange (ETDEWEB)

    Hernandes, Nilber Kenup; Vital, Helio de Carvalho [Centro Tecnologico do Exercito (CTEx), Rio de Janeiro, RJ (Brazil). Div. de Defesa Quimica, Biologica e Nuclear (DDQBN)]. E-mail: nilberkenup@ctex.eb.br; vital@ctex.eb.br; Coneglian, Regina Celi Cavestre [Universidade Federal Rural do Rio de Janeiro (UFRRJ), Seropedica, Rio de Janeiro, RJ (Brazil). Inst.de Agronomia. Dept. de Fitotecnia]. E-mail: rccconeg@ufrrj.br; Godoy, Ronoel Luiz de Oliveira [EMBRAPA Agroindustria de Alimentos, Rio de Janeiro, RJ (Brazil)]. E-mail: ronoel@ctaa.embrapa.br

    2007-07-01

    This work investigated the effects of gamma irradiation on the shelf-life extension and safety of minimally processed red beet (Beta vulgaris ssp. vulgaris L.) by performing microbiological, chemical and sensory analyses. Red beets were harvested 73 days after transplanting and their tuberous parts were minimally processed and separated in two groups: control (non-irradiated) and irradiated (0.5, 1.0 and 1.5 kGy). Tests for Salmonella sp., total and fecal coliforms, total count of aerobic mesophilic and lactic-acid bacteria were performed during the 21-day storage at 8 deg C. They indicated that the samples irradiated with 1.0 and 1.5 kGy remained in good conditions throughout storage while the unirradiated samples did not last 7 days. Chemical analyses indicated that the concentrations of vitamins B1 and B2 were not affected by irradiation. In contrast the amounts of fructose and glucose increased during storage while the one for sucrose decreased. In addition four series of sensory evaluations including appearance and aroma indicated that the samples irradiated with 1.0 and 1.5 kGy remained good for consumption for 20 days. Therefore it was concluded that the use of the doses of 1.0 and 1.5 kGy produced the best effects on the conservation of the samples without harming the sensory characteristics and nutritional constituents tested. (author)

  14. Genetic resources of Thymus vulgaris L. and T. vulgaris x T. Marschallianus Willd. in the Czech Republic

    Directory of Open Access Journals (Sweden)

    Duskova, Elena

    2016-07-01

    Full Text Available Two varieties of Thymus vulgaris L. (´Krajový´ and ´Winter´ and three its hybrids with T. Marschallianus Will. (variety ´Mixta´ and two accessions of variety ´Lemona´ were evaluated according the Draft Descriptor List Thymus vulgaris L. and analysed for the essential oil content and composition in years 2014 and 2015. All evaluated accessions were found morphologically and/or chemically different. Varieties ´Krajový´, ´Winter´ and ´Mixta´ were assessed as a thymol type with the thymol (39.1 – 69.6 %, o-cymene (6.3 – 24.9 % and γ-terpinene (2.75 – 13.8 % as main oil components. The two accessions of ´Lemona´ variety were found significantly different each other: one of them (income No. 3239 was assessed as a terpineol type with the terpineol acetate (75.7 % and α-terpineol (16.9 % as main oil components and the other one (income No. 2757 as a geraniol type with the geranyl acetate (±42.4 % and geraniol (±20.8 % as main oil components. Only the accession with income No. 3239 was proved as a ´Lemona´ variety due its citral (±9.3 % content, though even this content is too small compare to original ´Lemona´ where about 20 % of citral was declared.

  15. An alternative to estimate the atmospheric carbon sequestration by Bambusa vulgaris var. vulgaris Schrad. ex J.C. Wendl.

    Directory of Open Access Journals (Sweden)

    Alicia Mercadet

    2011-07-01

    Full Text Available The estimation of atmospheric carbon sequestration by a woody plant has two alternatives for implementation: by identifying the average annual increase in volume or by determining the appropriate method for calculating the volume stand. The aim of this work was to obtain a reliable and simple method for estimating the carbon in plants and culms of bamboo (Bambusa vulgaris var. vulgaris existing in a territory. Single samples were taken without replacement in different areas and each culm was measured in total length, outside diameter and wall thickness every two meters from the base to the segment in the upper left to present an interior cavity identifiable, in which the remaining length to the top was measured. Graphical analyses were conducted with the data collected of trends in relations between the variables considered along the culm. By regression analysis with different models and the use of determination coefficient (R2 as an adjustment indicator, we selected those that best allow estimating the solid volume of the culm. After obtaining the calibration models and their results, we prepared a spreadsheet that facilitates to take down the values of the different variables by culm and scion as well as the characterization of the scion average culm. The results are a first attempt to develop a method for estimating the carbon sequestered by the bamboo culm at both as scion and territory to include this element among the criteria for the management plans of this non-wood forest resource. Key words: allometric models, bamboo, regression analysis

  16. Primer registro de Vespula vulgaris (Hymenoptera: Vespidae en la Argentina First record of Vespula vulgaris (Hymenoptera: Vespidae in Argentina

    Directory of Open Access Journals (Sweden)

    Maité Masciocchi

    2010-12-01

    Full Text Available Vespula vulgaris (Linnaeus es un véspido social nativo de la región Holártica. En este trabajo reportamos la primera detección de esta especie en Argentina. Obreras de esta avispa fueron capturadas cerca de la ciudad de San Carlos de Bariloche (Argentina en Febrero de 2010, mientras se tomaban muestras de otra avispa invasora, Vespula germanica (Fabricius o chaqueta amarilla, de morfología externa y hábitos similares a la anteriormente mencionada. Además, detallamos algunos caracteres de identificación y características biológicas.Vespula vulgaris (Linnaeus is a social vespid native to the Holarctic region. The first detection of this species in Argentina is here reported. Workers were captured close to San Carlos de Bariloche (Argentina during February 2010, while sampling for another successful invader, the German wasp or Yellowjacket, Vespula germanica (Fabricius. Both these wasp species are very similar morphologically and share a number of common habits. Also, some identification features and biological characters are here explained.

  17. Rhizofiltration using sunflower (Helianthus annuus L.) and bean (Phaseolus vulgaris L. var. vulgaris) to remediate uranium contaminated groundwater

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Minhee, E-mail: heelee@pknu.ac.kr [Department of Environmental Geosciences, Pukyong National University, 599-1 Daeyondong, Namgu, Busan 608-737 (Korea, Republic of); Yang, Minjune [Department of Environmental Geosciences, Pukyong National University, 599-1 Daeyondong, Namgu, Busan 608-737 (Korea, Republic of)

    2010-01-15

    The uranium removal efficiencies of rhizofiltration in the remediation of groundwater were investigated in lab-scale experiments. Sunflower (Helianthus annuus L.) and bean (Phaseolus vulgaris L. var. vulgaris) were cultivated and an artificially uranium contaminated solution and three genuine groundwater samples were used in the experiments. More than 80% of the initial uranium in solution and genuine groundwater, respectively, was removed within 24 h by using sunflower and the residual uranium concentration of the treated water was lower than 30 {mu}g/L (USEPA drinking water limit). For bean, the uranium removal efficiency of the rhizofiltration was roughly 60-80%. The maximum uranium removal via rhizofiltration for the two plant cultivars occurred at pH 3-5 of solution and their uranium removal efficiencies exceeded 90%. The lab-scale continuous rhizofiltration clean-up system delivered over 99% uranium removal efficiency, and the results of SEM and EDS analyses indicated that most uranium accumulated in the roots of plants. The present results suggested that the uranium removal capacity of two plants evaluated in the clean-up system was about 25 mg/kg of wet plant mass. Notably, the removal capacity of the root parts only was more than 500 mg/kg.

  18. Rhizofiltration using sunflower (Helianthus annuus L.) and bean (Phaseolus vulgaris L. var. vulgaris) to remediate uranium contaminated groundwater.

    Science.gov (United States)

    Lee, Minhee; Yang, Minjune

    2010-01-15

    The uranium removal efficiencies of rhizofiltration in the remediation of groundwater were investigated in lab-scale experiments. Sunflower (Helianthus annuus L.) and bean (Phaseolus vulgaris L. var. vulgaris) were cultivated and an artificially uranium contaminated solution and three genuine groundwater samples were used in the experiments. More than 80% of the initial uranium in solution and genuine groundwater, respectively, was removed within 24h by using sunflower and the residual uranium concentration of the treated water was lower than 30 microg/L (USEPA drinking water limit). For bean, the uranium removal efficiency of the rhizofiltration was roughly 60-80%. The maximum uranium removal via rhizofiltration for the two plant cultivars occurred at pH 3-5 of solution and their uranium removal efficiencies exceeded 90%. The lab-scale continuous rhizofiltration clean-up system delivered over 99% uranium removal efficiency, and the results of SEM and EDS analyses indicated that most uranium accumulated in the roots of plants. The present results suggested that the uranium removal capacity of two plants evaluated in the clean-up system was about 25mg/kg of wet plant mass. Notably, the removal capacity of the root parts only was more than 500 mg/kg.

  19. Retinal oscillations carry visual information to cortex

    Directory of Open Access Journals (Sweden)

    Kilian Koepsell

    2009-04-01

    Full Text Available Thalamic relay cells fire action potentials that transmit information from retina to cortex. The amount of information that spike trains encode is usually estimated from the precision of spike timing with respect to the stimulus. Sensory input, however, is only one factor that influences neural activity. For example, intrinsic dynamics, such as oscillations of networks of neurons, also modulate firing pattern. Here, we asked if retinal oscillations might help to convey information to neurons downstream. Specifically, we made whole-cell recordings from relay cells to reveal retinal inputs (EPSPs and thalamic outputs (spikes and then analyzed these events with information theory. Our results show that thalamic spike trains operate as two multiplexed channels. One channel, which occupies a low frequency band (<30 Hz, is encoded by average firing rate with respect to the stimulus and carries information about local changes in the visual field over time. The other operates in the gamma frequency band (40-80 Hz and is encoded by spike timing relative to retinal oscillations. At times, the second channel conveyed even more information than the first. Because retinal oscillations involve extensive networks of ganglion cells, it is likely that the second channel transmits information about global features of the visual scene.

  20. Testability Synthesis for Jumping Carry Adders

    Directory of Open Access Journals (Sweden)

    Chien-In Henry Chen

    2002-01-01

    Full Text Available Synthesis for testability ensures that the synthesized circuit is testable by exploring the fundamental relationship between don't care and redundancy. With the exploration of the relationship, redundancy removal can be applied to improve the testability, reduce the area and improve the speed of a synthesized circuit. The test generation problems have been adequately solved, therefore an innovative testability synthesis strategy is necessary for achieving the maximum fault coverage and area reduction for maximum speed. This paper presents a testability synthesis methodology applicable to a top–down design method based on the identification and removal of redundant faults. Emphasis has been placed on the testability synthesis of a high-speed binary jumping carry adder. A synthesized 32-bit testable adder implemented by a 1.2 μm CMOS technology performs addition in 4.09 ns. Comparing with the original synthesized circuit, redundancy removal yields a 100% testable design with a 15% improvement in speed and a 25% reduction in area.

  1. Robust ferromagnetism carried by antiferromagnetic domain walls

    Science.gov (United States)

    Hirose, Hishiro T.; Yamaura, Jun-Ichi; Hiroi, Zenji

    2017-02-01

    Ferroic materials, such as ferromagnetic or ferroelectric materials, have been utilized as recording media for memory devices. A recent trend for downsizing, however, requires an alternative, because ferroic orders tend to become unstable for miniaturization. The domain wall nanoelectronics is a new developing direction for next-generation devices, in which atomic domain walls, rather than conventional, large domains themselves, are the active elements. Here we show that atomically thin magnetic domain walls generated in the antiferromagnetic insulator Cd2Os2O7 carry unusual ferromagnetic moments perpendicular to the wall as well as electron conductivity: the ferromagnetic moments are easily polarized even by a tiny field of 1 mT at high temperature, while, once cooled down, they are surprisingly robust even in an inverse magnetic field of 7 T. Thus, the magnetic domain walls could serve as a new-type of microscopic, switchable and electrically readable magnetic medium which is potentially important for future applications in the domain wall nanoelectronics.

  2. Mixotrophic growth and biochemical analysis of Chlorella vulgaris cultivated with diluted monosodium glutamate wastewater.

    Science.gov (United States)

    Ji, Yan; Hu, Wenrong; Li, Xiuqing; Ma, Guixia; Song, Mingming; Pei, Haiyan

    2014-01-01

    Monosodium glutamate wastewater (MSGW) is a potential medium for microbial cultivation because of containing abundant organic nutrient. This paper seeks to evaluate the feasibility of growing Chlorella vulgaris with MSGW and assess the influence of MSGW concentration on the biomass productivity and biochemical compositions. The MSGW diluted in different concentrations was prepared for microalga cultivation. C. vulgaris growth was greatly promoted with MSGW compared with the inorganic BG11 medium. C. vulgaris obtained the maximum biomass concentration (1.02 g/L) and biomass productivity (61.47 mg/Ld) with 100-time diluted MSGW. The harvested biomass was rich in protein (36.01-50.64%) and low in lipid (13.47-25.4%) and carbohydrate (8.94-20.1%). The protein nutritional quality and unsaturated fatty acids content of algal increased significantly with diluted MSGW. These results indicated that the MSGW is a feasible alternative for mass cultivation of C. vulgaris.

  3. Influence of plaque-forming bacterium, Rhodobacteraceae sp. on the growth of Chlorella vulgaris.

    Science.gov (United States)

    Chen, Zhangran; Zhang, Jingyan; Lei, Xueqian; Zhang, Bangzhou; Cai, Guanjing; Zhang, Huajun; Li, Yi; Zheng, Wei; Tian, Yun; Xu, Hong; Zheng, Tianling

    2014-10-01

    Experiments were conducted to find out the molecular features, infection process of a special alga plaque-forming microorganism and its potential influence on the biomass of Chlorella vulgaris during the infection process. Direct contact between the algal cell and the bacterium may be the primary steps needed for the bacterium to lyse the alga. Addition of C. vulgaris cells into f/2 medium allowed us obtain the object bacterium. The 16S rRNA gene sequence comparisons results showed that the plaque-forming bacterium kept the closest relationship with Labrenzia aggregata IAM 12614(T) at 98.90%. The existence of the bacterium could influence both the dry weight and lipid content of C. vulgaris. This study demonstrated that direct cell wall disruption of C. vulgaris by the bacterium would be a potentially effective method to utilize the biomass of microalgae.

  4. Th-17 and the lack of efficacy of ustekinumab in pemphigus vulgaris.

    Science.gov (United States)

    Tirado-Sánchez, Andrés; Ponce-Olivera, Rosa María; Vazquez-González, Denisse; Bonifaz, Alexandro

    2013-03-15

    Systemic corticosteroids represent an effective treatment for pemphigus vulgaris (PV). However, this treatment is related to many adverse side effects. Herein, we report a case of PV treated with ustekinumab.

  5. Cranial Mesenteric Arterial Obstruction Due To Strongylus vulgaris Larvae in a Donkey (Equus asinus.

    Directory of Open Access Journals (Sweden)

    Hassan Borji

    2014-09-01

    Full Text Available Arteritis due to Strongylus vulgaris is a well-known cause of colic in horses and donkeys. The current report describes a fatal incidence of arterial obstruction in cranial mesenteric artery caused by S. vulgaris infection in an adult donkey in which anthelmintic treatment was not regularly administered. Necropsy findings of the abdominal cavity revealed a complete cranial mesenteric arterial obstruction due to larvae of S. vulgaris, causing severe colic. To the authors' knowledge, a complete cranial mesenteric arterial obstruction due to verminous arteritis has rarely been described in horses and donkeys. Based on recent reports of fatal arterial obstruction due to S. vulgaris infection in donkeys, it may be evident to consider acute colic caused by this pathogenic parasite a re-emerging disease in donkeys and horses.

  6. Composition and antimicrobial studies of essential oil of Thymus vulgaris from Montenegro

    Directory of Open Access Journals (Sweden)

    Damjanović-Vratnica, B.

    2015-12-01

    Full Text Available Chemical composition of the hydrodistilled essential oil of Thymus vulgaris L. (thyme from Montenegro was analyzed by gas chromatography-mass spectrometry and its antimicrobial activity was evaluated against 10 microorganisms, including reference and clinically isolated strains. T. vulgaris essential oil yield was 0.42% (v/w, based on the dry leaves weight whereas the analysis showed that major components, amongst 22 identified in the oil, were geraniol (25.66%, geranyl-acetate (20.34%, linalool (10.89% and caryophyllene oxide (9.89%. The results of the antimicrobial activity tests revealed that the essential oil of T .vulgaris from Montenegro has rather strong antimicrobial activity, especially against Staphylococcus aureus, Escherichia coli, Candida albicans and Klebsiella pneumoniae. These results confirm the potential use of T. vulgaris essential oil in food products the as well as for therapeutic applications.

  7. Synergistic antibacterial activity between Thymus vulgaris and Pimpinella anisum essential oils and methanol extracts.

    Science.gov (United States)

    Al-Bayati, Firas A

    2008-03-28

    Essential oils (EOs) and methanol extracts obtained from aerial parts of Thymus vulgaris and Pimpinella anisum seeds were evaluated for their single and combined antibacterial activities against nine Gram-positive and Gram-negative pathogenic bacteria: Staphylococcus aureus, Bacillus cereus, Escherichia coli, Proteus vulgaris, Proteus mirabilis, Salmonella typhi, Salmonella typhimurium, Klebsiella pneumoniae and Pseudomonas aeruginosa. The essential oils and methanol extracts revealed promising antibacterial activities against most pathogens using broth microdilution method. Maximum activity of Thymus vulgaris and Pimpinella anisum essential oils and methanol extracts (MIC 15.6 and 62.5mug/ml) were observed against Staphylococcus aureus, Bacillus cereus and Proteus vulgaris. Combinations of essential oils and methanol extracts showed an additive action against most tested pathogens especially Pseudomonas aeruginosa.

  8. Lupus Vulgaris Erythematoides: report of a patient initially misdiagnosed as dermatitis.

    Science.gov (United States)

    Vazquez-Lopez, Francisco; Fueyo-Casado, Alejandro; Gonzalez-Lara, Leire

    2013-05-15

    A small percentage of patients with tuberculosis present with cutaneous findings, which may be difficult to diagnose. We present a patient diagnosed with a rare, non-scarring form of cutaneous tuberculosis (CTB), classically termed as lupus vulgaris erythematoides.

  9. Induction of tryptophanase in short cells and swarm cells of Proteus vulgaris.

    OpenAIRE

    Hoffman, P S; Falkinham, J O

    1981-01-01

    Tryptophanase was noninducible in swarm cells of Proteus vulgaris despite transport of the inducer tryptophan. Further, cyclic AMP, which stimulated increased levels of tryptophanase in short cells, had no effect on swarm cells.

  10. Octopus vulgaris (Cuvier, 1797) in the Mediterranean Sea: Genetic Diversity and Population Structure: e0149496

    National Research Council Canada - National Science Library

    Daniele De Luca; Gaetano Catanese; Gabriele Procaccini; Graziano Fiorito

    2016-01-01

      The common octopus, Octopus vulgaris Cuvier 1797, is a largely exploited cephalopod species in the Mediterranean Sea and the Atlantic Ocean, as well as along the coasts of Africa, Brazil and Japan...

  11. Transcriptome analysis of salt tolerant common bean (Phaseolus vulgaris L.) under saline conditions

    National Research Council Canada - National Science Library

    Hiz, Mahmut Can; Canher, Balkan; Niron, Harun; Turet, Muge

    2014-01-01

    .... Common bean, Phaseolus vulgaris L., a major protein source in developing countries, is highly affected by soil salinity and the information on genes that play a role in salt tolerance is scarce...

  12. Serological diagnosis of Strongylus vulgaris infection: use of a recombinant protein

    DEFF Research Database (Denmark)

    Andersen, Ulla Vestergaard; Howe, Daniel K.; Olsen, Susanne Nautrup

    , an immunoreactive cDNA clone was subcloned into E. coli and the plasmid sequenced, the open reading frame encoding the mature protein was cloned into a pET22b expression vector and expressed as a His-tagged recombinant protein in BL21 expression cells. The recombinant protein was used in an indirect enzyme...... the pathogenic migrating larval stages of S. vulgaris ante mortem. A cDNA library was constructed from RNA extracted from migrating S. vulgaris larvae. Excretory-secretory antigens from S. vulgaris adult specimens were used to immunise a rat. Hyperimmune serum was used to immunoscreen the cDNA library....... vulgaris (n=9) reacted against the recombinant protein, expressed as optic density (OD) readings of >24 % of a positive control, while sera from negative horses had OD readings

  13. Efficient carry skip Adder design using full adder and carry skip block based on reversible Logic

    Directory of Open Access Journals (Sweden)

    Varun Pratap Singh

    2015-12-01

    Full Text Available In recent years, Reversible Logic is becoming more and more prominent technology having its applications in Quantum Computing, Nanotechnology, and Optical Computing. Reversibility plays an important role when energy efficient computations are considered. In this paper, binary full Adder with Design I and Design II are proposed. The performance analysis is verified using number of reversible gates, Garbage input/outputs, delay, number of logical calculations and Quantum Cost. According to the suitability of full adder design I and design II carry skip adder block is also constructed with some improvement in terms of delay in block carry generation. It is observed that Reversible carry skip Binary Adder with Design II is efficient compared to Design I

  14. Medicinal Plants for the Treatment of Acne Vulgaris: A Review of Recent Evidences

    OpenAIRE

    Nasri, Hamid; Bahmani, Mahmoud; Shahinfard, Najmeh; Moradi Nafchi, Atefeh; Saberianpour, Shirin; Rafieian Kopaei, Mahmoud

    2015-01-01

    Context: Acne vulgaris affects about 85% of teenagers and may continue to adulthood. There are about two million visits to physicians per year for teenagers and the direct cost of acne treatment in the US exceeds $1 billion per year. Evidence Acquisition: A wide variety of treatment regimens exist for acne vulgaris including benzoil peroxide, retinoids, isotretinoids, keratolytic soaps, alpha hydroxy acids, azelaic acid, salicilic acid as well as hormonal, anti-androgen or antiseborrheic trea...

  15. Clinical cross-reactivity between Artemisia vulgaris and Matricaria chamomilla (chamomile).

    Science.gov (United States)

    de la Torre Morín, F; Sánchez Machín, I; García Robaina, J C; Fernández-Caldas, E; Sánchez Triviño, M

    2001-01-01

    Artemisia vulgaris is a common weed and an important source of allergens on the subtropical island of Tenerife, Canary Islands, Spain. It pollinates mainly from July to September, although, due to some local climatic conditions, it may flower throughout the year. Cross-reactivity with hazelnut, kiwi, birch, several Compositae (Ambrosia, Chrysanthemum, Matricaria, Solidago) and grass allergens has been suggested. Few studies have addressed the issue of in vivo cross-reactivity between A. vulgaris and Matricaria chamomilla. The objective of this study was to perform conjunctival and bronchial challenges with A. vulgaris and M. chamomilla and oral challenge with chamomile in 24 patients with asthma and/or rhinitis sensitized primarily to A. vulgaris. Skin prick tests with M. chamomilla were positive in 21 patients. Eighteen patients had a positive conjunctival provocation test with a A. vulgaris pollen extract and 13 patients had a positive conjunctival provocation test with a M. chamomilla pollen extract. Bronchial provocation tests with A. vulgaris were positive in 15 patients and with M. chamomilla pollen in another 16 individuals. Oral provocation tests, conducted with a commercial chamomile infusion were positive in 13 patients. Nine of these individuals were skin test positive to food allergens and 17 to others pollens of the Compositae family. This study confirms a high degree of in vivo cross-reactivity between A. vulgaris and M. chamomilla. Sensitization to A. vulgaris seems to be a primary risk factor for experiencing symptoms after the ingestion of chamomile infusions. Based on the results of bronchial provocation tests, M. chamomilla pollen could be a relevant inhalant allergen.

  16. Clinical characteristics and epidermal barrier function of papulopustular rosacea: A comparison study with acne vulgaris

    OpenAIRE

    Zhou, Maosong; Xie, Hongfu; Cheng, Lin; Li, Ji

    2016-01-01

    Objective: To evaluate the clinical characteristics and epidermal barrier function of papulopustular rosacea by comparing with acne vulgaris. Methods: Four hundred and sixty-three papulopustular rosacea patients and four hundred and twelve acne vulgaris patients were selected for the study in Xiangya Hospital of Central South University from March 2015 to May 2016. They were analyzed for major facial lesions, self-conscious symptoms and epidermal barrier function. Results: Erythema, burning, ...

  17. The genome sequence of Barbarea vulgaris facilitates the study of ecological biochemistry

    DEFF Research Database (Denmark)

    Byrne, Stephen L.; Erthmann, Pernille Østerbye; Agerbirk, Niels

    2017-01-01

    The genus Barbarea has emerged as a model for evolution and ecology of plant defense compounds, due to its unusual glucosinolate profile and production of saponins, unique to the Brassicaceae. One species, B. vulgaris, includes two ‘types’, G-type and P-type that differ in trichome density, and t...... deter larvae to the extent that they die. The B. vulgaris genome will promote the study of mechanisms in ecological biochemistry to benefit crop resistance breeding....

  18. Identification of an Alternative to Proteus vulgaris as a Laboratory Standard for Hydrogen Sulfide Production

    Directory of Open Access Journals (Sweden)

    Nar'Asha Randall

    2017-05-01

    Full Text Available This project involved the evaluation of a biosafety level 1 alternative to Proteus vulgaris as a positive control for the production of hydrogen sulfide. We determined that Citrobacter freundii could serve as an excellent substitute for P. vulgaris, and that lead acetate strips used in conjunction with triple sugar iron media allows for consistent results following evaluation after up to one week.

  19. XML Investigating the Phytochemical, Antibacterial and Antifungal Effects of Thymus Vulgaris and Cuminum Cyminum Essential Oils

    OpenAIRE

    2016-01-01

    Background and Objective: The aim of this study was to determine the chemical composition, antibacterial and antifungal effects of Thymus vulgaris and Cuminum Cyminum essential oils against foodborne pathogens and Candida species in vitro. Methods: The essential oils were extracted from the aerial parts of Thymus vulgaris and dried Cuminum Cyminum seeds using a Clevenger apparatus for 3 hours. Analysis of the essential oils’ constituents was performed using gas chromatography-mass s...

  20. DRY CALCAREOUS GRASSLAND COMMUNITIES (FILIPENDULA VULGARIS-HELICTOTRICHON PRATENSE) IN WESTERN AND CENTRAL LATVIA

    OpenAIRE

    S. RUSINA

    2003-01-01

    The dry calcareous grassland vegetation of Westem and Central Latvia is described based on 93 rclevés, Ali relevés could be assigned to one community type Filipendula vulgaris-Helictotrichon pratense named according to dominant species. Four variants were distinguished: typicum, Viscaria vulgaris, Astragalus danicus and Carex flacca. Ellenberg indìcator values were calculated to study the ecology of communities. Floristic differences among variants are associated mainly w...

  1. Dispersal of Calluna vulgaris (L. Hull. seeds on severely burnt upland moorland

    Directory of Open Access Journals (Sweden)

    J.A. Gilbert

    2010-07-01

    Full Text Available From natural and manipulated experiments at 460 m a.s.l., traps were used to record the dispersal range of Calluna vulgaris seeds on an unvegetated post-wildfire site. Seeds were trapped to 50 m from source, although most (73% were deposited within one metre of the parent plant. C. vulgaris seeds were not dispersed as far in these experiments as recorded in previous workers’ experiments on lowland grassland.

  2. DRY CALCAREOUS GRASSLAND COMMUNITIES (FILIPENDULA VULGARIS-HELICTOTRICHON PRATENSE) IN WESTERN AND CENTRAL LATVIA

    OpenAIRE

    Rusina, S.

    2003-01-01

    The dry calcareous grassland vegetation of Westem and Central Latvia is described based on 93 rclevés, Ali relevés could be assigned to one community type Filipendula vulgaris-Helictotrichon pratense named according to dominant species. Four variants were distinguished: typicum, Viscaria vulgaris, Astragalus danicus and Carex flacca. Ellenberg indìcator values were calculated to study the ecology of communities. Floristic differences among variants are associa...

  3. Therapeutic plasma exchange-A new dawn in the treatment of pemphigus vulgaris

    Directory of Open Access Journals (Sweden)

    Rajesh Kumar

    2015-01-01

    Full Text Available Pemphigus vulgaris is an autoimmune disorder that involves intraepithelial blistering and sores of skin and mucous membrane. It generally correlates with the levels of circulating autoantibodies; their removal seems a reasonable therapeutic approach. Therapeutic plasma exchange is hypothesized to remove pathogenic autoantibodies and has been used in refractory or severe cases. It may be considered for rapid control of severe or recalcitrant pemphigus vulgaris and should be combined with use of concomitant immuno-suppressive.

  4. Two-Component Signal Transduction Systems of Desulfovibrio Vulgaris: Structural and Phylogenetic Analysis and Deduction of Putative Cognate Pairs

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Weiwen; Culley, David E.; Wu, Gang; Brockman, Fred J.

    2006-01-20

    ABSTRACT-Two-component signal transduction systems (TCSTS) composed of sensory histidine kinases (HK) and response regulators (RR), constitute a key element of the mechanism by which bacteria sense and respond to changes in environments. A large number of TCSTSs including 59 putative HKs and 55 RRs were identified from the Desulfovibrio vulgaris genome, indicating their important roles in regulation of cellular metabolism. In this study, the structural and phylogenetic analysis of all putative TCSTSs in D. vulgaris was performed. The results showed D. vulgaris contained an unexpectedly large number of hybrid-type HKs, implying that multiple-step phosphorelay may be a common signal transduction mechanism in D. vulgaris. Most TCSTS components of D. vulgaris were found clustered into several subfamilies previously recognized in other bacteria and extensive co-evolution between D. vulgaris HKs and RRs was observed, suggesting that the concordance of HKs and RRs in cognate phylogenetic groups could be indicative of cognate TCSTSs...

  5. Study of the effect of extract of Thymus vulgaris on anxiety in male rats.

    Science.gov (United States)

    Komaki, Alireza; Hoseini, Faeghe; Shahidi, Siamak; Baharlouei, Negar

    2016-07-01

    There is some evidence in traditional medicine for the effectiveness of Thymus vulgaris ( bǎi lǐ xiāng) in the treatment of anxiety in humans. The elevated plus-maze (EPM) has broadly been used to investigate anxiolytic and anxiogenic compounds. The present study investigated the effects of extract of T. vulgaris on rat behavior in the EPM. In the present study, the data were obtained from male Wistar rats. Animals were divided into four groups: saline group and T. vulgaris groups (50 mg/kg, 100 mg/kg, and 200 mg/kg infusion for 7 days by feeding). During the test period, the total distance covered by animals, the number of open- and closed-arm entries, and the time spent in open and closed arms of the EPM were recorded. T. vulgaris increased open-arm exploration and open-arm entry in the EPM, whereas extract of this plant has no effects on the total distance covered by animals and the number of closed-arm entries. The results of the present experiment indicate that T. vulgaris may have an anxiolytic profile in rat behavior in the EPM test, which is not influenced by the locomotor activity. Further research is required to determine the mechanisms by which T. vulgaris extract exerts an anxiolytic effect in rats.

  6. Prevalence and risk factors of facial acne vulgaris among Chinese adolescents.

    Science.gov (United States)

    Wu, Tie-Qang; Mei, Shu-Qing; Zhang, Jin-Xin; Gong, Long-Fen; Wu, Fen-Jin; Wu, Wei-Hong; Li, Jun; Lin, Min; Diao, Jian-Xing

    2007-01-01

    Acne vulgaris, a very common condition among adolescents, can result in psychological distress, including anxiety and depression. The purpose of this study was to assess the prevalence and risk factors of acne vulgaris among Chinese adolescents. In the Zhou Hai district of Guangdong Province, 3163 students 10 to 18 years old were selected from 7 schools. Information was collected using self-administrated questionnaIres and physician examinations. The prevalence of acne vulgaris was calculated from the collected data. Potential risk factors including age, gender, diet, skin type, sleeping habits, and facial make-up use were analyzed using stepwise logistic regression. The results showed a prevalence of acne vuglaris of 53.5% in all adolescents, with 51.3% in males and 58.6% in females. The prevalence of inflammatory acne in males and females combined was 25.8% and of acne scarring 7.1%. Increased age was related to higher prevalence and severity of acne vulgaris: 15.6%, 44.9%, and 70.4% for 10, 13, and 16 year olds, respectively. Acne vulgaris was more prevalent in girls under and boys over 14 years of age. Significant risk factors of acne vuglaris included age, skin type (oily, mixed, or neutral skin in comparison with dry skin), insufficient sleep, and cosmetic make-up use. Acne vulgaris is prevalent among Chinese adolescents 10 to 18 years old. Health education to address this condition in Chinese adolescents could have important implications for public health.

  7. Polyphosphate during the Regreening of Chlorella vulgaris under Nitrogen Deficiency.

    Science.gov (United States)

    Chu, Fei-Fei; Shen, Xiao-Fei; Lam, Paul K S; Zeng, Raymond J

    2015-09-28

    Polyphosphate (Poly-P) accumulation has been reported in Chlorella vulgaris under nitrogen deficiency conditions with sufficient P supply, and the process has been demonstrated to have great impact on lipid productivity. In this article, the utilization of polyphosphates and the regreening process under N resupplying conditions, especially for lipid production reviving, were investigated. This regreening process was completed within approximately 3-5 days. Polyphosphates were first degraded within 3 days in the regreening process, with and without an external P supply, and the degradation preceded the assimilation of phosphate in the media with an external P offering. Nitrate assimilation was markedly influenced by the starvation of P after polyphosphates were exhausted in the medium without external phosphates, and then the reviving process of biomass and lipid production was strictly impeded. It is, thus, reasonable to assume that simultaneous provision of external N and P is essential for overall biodiesel production revival during the regreening process.

  8. Polyphosphate during the Regreening of Chlorella vulgaris under Nitrogen Deficiency

    Directory of Open Access Journals (Sweden)

    Fei-Fei Chu

    2015-09-01

    Full Text Available Polyphosphate (Poly-P accumulation has been reported in Chlorella vulgaris under nitrogen deficiency conditions with sufficient P supply, and the process has been demonstrated to have great impact on lipid productivity. In this article, the utilization of polyphosphates and the regreening process under N resupplying conditions, especially for lipid production reviving, were investigated. This regreening process was completed within approximately 3–5 days. Polyphosphates were first degraded within 3 days in the regreening process, with and without an external P supply, and the degradation preceded the assimilation of phosphate in the media with an external P offering. Nitrate assimilation was markedly influenced by the starvation of P after polyphosphates were exhausted in the medium without external phosphates, and then the reviving process of biomass and lipid production was strictly impeded. It is, thus, reasonable to assume that simultaneous provision of external N and P is essential for overall biodiesel production revival during the regreening process.

  9. Cannibalistic behavior of octopus (Octopus vulgaris) in the wild.

    Science.gov (United States)

    Hernández-Urcera, Jorge; Garci, Manuel E; Roura, Alvaro; González, Angel F; Cabanellas-Reboredo, Miguel; Morales-Nin, Beatriz; Guerra, Angel

    2014-11-01

    The first description of cannibalism in wild adult Octopus vulgaris is presented from 3 observations made in the Ría de Vigo (NW Spain), which were filmed by scuba divers. These records document common traits in cannibalistic behavior: (a) it was intercohort cannibalism; (b) attacks were made by both males and females; (c) in 2 of the records, the prey were transported to the den, which was covered with stones of different sizes; (d) the predator started to eat the tip of the arms of its prey; (e) predation on conspecifics occurred even if there were other abundant prey available (i.e., mussels); and (f) the prey/predator weight ratio in the 3 cases ranged from 20% to 25% body weight. The relationships between this behavior and sex, defense of territory, energy balance, food shortage, competition and predation, as well as how the attacker kills its victim are discussed.

  10. Physiological and biochemical responses of Chlorella vulgaris to Congo red.

    Science.gov (United States)

    Hernández-Zamora, Miriam; Perales-Vela, Hugo Virgilio; Flores-Ortíz, César Mateo; Cañizares-Villanueva, Rosa Olivia

    2014-10-01

    Extensive use of synthetic dyes in many industrial applications releases large volumes of wastewater. Wastewaters from dying industries are considered hazardous and require careful treatment prior to discharge into receiving water bodies. Dyes can affect photosynthetic activities of aquatic flora and decrease dissolved oxygen in water. The aim of this study was to evaluate the effect of Congo red on growth and metabolic activity of Chlorella vulgaris after 96h exposure. Exposure of the microalga to Congo red reduced growth rate, photosynthesis and respiration. Analysis of chlorophyll a fluorescence emission showed that the donor side of photosystem II was affected at high concentrations of Congo red. The quantum yield for electron transport (φEo), the electron transport rate (ETR) and the performance index (PI) also decreased. The reduction in the ability to absorb and use the quantum energy increased non-photochemical (NPQ) mechanisms for thermal dissipation. Overall, Congo red affects growth and metabolic activity in photosynthetic organisms in aquatic environments.

  11. Pemphigus Vulgaris Confined to the Gingiva: A Case Report

    Directory of Open Access Journals (Sweden)

    Mitsuhiro Ohta

    2011-01-01

    Full Text Available Pemphigus Vulgaris (PV is an autoimmune intraepithelial blistering disease involving the skin and mucous membranes. Oral mucosa is frequently affected in patients with PV, and oral lesions may be the first sign of the disease in majority of patients. In some patients, oral lesions may also be followed by skin involvement. Therefore, timely recognition and therapy of oral lesions is critical as it may prevent skin involvement. Early oral lesions of PV are, however, often regarded as difficult to diagnose, since the initial oral lesions may be relatively nonspecific, manifesting as superficial erosions or ulcerations, and rarely presenting with the formation of intact bullae. Lesions may occur anywhere on the oral mucosa including gingiva; however; desquamtive gingivitis is less common with PV than other mucocutaneous conditions such as pemphigoid or lichen planus. This paper describes the case of a patient presenting with a one-year history of painful gingival, who is finally diagnosed as having PV.

  12. A Novel Rhodopsin Gene from Octopus vulgaris for Optobioelectronics Materials

    Directory of Open Access Journals (Sweden)

    Alexander Zhgun

    2015-06-01

    Full Text Available The unique photochromic retinal protein from rhabdomeric octopus membranes – octopus rhodopsin (OctR has emerged as promising material for biomolecular photonic applications due to its unique properties and advantages. Here we report isolation of the novel full length octR gene from retina cDNA of Octopus vulgaris eyes and its sequence comparison with rhodopsins of other cephalopods and vertebrates. The isolated gene can be used to develop various expression systems for production of recombinant OctR for structural studies and novel optobioelectronic applications. The alignment of amino acid (a.a. sequence with different opsins revealed similarity to cephalopoda rhodopsins (Rho and to human melanopsin from intrinsically photosensitive retinal ganglion cells. The alingment of OctR a.a. sequence with mammalian and cephalopoda Rho with known 3D structures revealed promising substitutions V2C and W292C for developing stable and functionally active recombinant OctR after heterologous expression.

  13. Antibacterial activity of Beta vulgaris L. pomace extract

    Directory of Open Access Journals (Sweden)

    Velićanski Aleksandra S.

    2011-01-01

    Full Text Available Antibacterial activity of Beta vulgaris L. (beetroot pomace extract (concentration 100 mg/ml was tested against five Gram positive and seven Gram negative bacterial strains (reference cultures and natural isolates. Disc diffusion method with 15 µl of extract and agar-well diffusion method with 50 and 100 µl were used. Antibiotic (cefotaxime/clavulanic acid was used as a control sample. The tested extract showed the highest activity against Staphylococcus aureus and Bacillus cereus, where clear zones (without growth appeared. There was no any activity against other tested Gram-positive bacteria, except for Staphylococcus epidermidis, with a small zone of reduced growth. Growth of all tested Gram-negative bacteria was inhibited usually with 100 µl of extract. The most susceptible were Citrobacter freundii and Salmonella typhymurium. The tested antibiotic gave clear, usually large zones for all tested strains except for Staphylococcus cohni spp. cohni, where only a zone of reduced growth appeared.

  14. Treatment Of ACNE Vulgaris With New Polyherbal Formulations

    Directory of Open Access Journals (Sweden)

    Sachidananda Y N

    2001-01-01

    Full Text Available One hundred and five patients with active lesions of acne vulgaris were included in the open clinical trial. All the patients were administered tablets containing multiple herbal drugs (purim, at a dose of 2 tablets twice daily for four weeks. Simultaneously, they were instructed to apply A herbal cream (Clarina twice daily on the affected area of acne lesion twice daily for four weeks. The response to treatment was excellent in mild to moderate acne (Grade I & II after 4 weeks of treatment. In severe acne with large papules and pustules, the response was also significantly good, in healing the papules and pustules. There were no local or systemic side effects seen in all these patients. Thus, this polyherbal cream (Clarina along with a polyherbal tablet (Purim tablets was useful in treating patients with various degrees of acne.

  15. Oxidation of Octopus vulgaris hemocyanin by nitrogen oxides

    Energy Technology Data Exchange (ETDEWEB)

    Salvato, B.; Giacometti, G.M.; Beltramini, M.; Zilio, F.; Giacometti, G.; Magliozzo, R.S.; Peisach, J.

    1989-01-24

    The reaction of Octopus vulgaris hemocyanin with nitrite was studied under a variety of conditions in which the green half-met derivative is formed. Analytical evidence shows that the amount of chemically detectable nitrite in various samples of the derivative is not proportional to the cupric copper detected by EPR. The kinetics of oxidation of hemocyanin as a function of protein concentration and pH, in the presence of nitrite and ascorbate, is consistent with a scheme in which NO/sub 2/ is the reactive oxidant. We suggest that the green half-methemocyanin contains a metal center with one cuprous and one cupric copper without an exogenous nitrogen oxide ligand.

  16. Mapping the Two-component Regulatory Networks in Desulfovibrio vulgaris

    Energy Technology Data Exchange (ETDEWEB)

    Rajeev, Lara; Luning, Eric; Dehal, Paramvir; Joachimiak, Marcin; Mukhopadhyay, Aindrila

    2010-05-17

    D. vulgaris Hildenborough has 72 response regulators. The Desulfovibrio are sulfate reducing bacteria that are important in the sulfur and carbon cycles in anoxic habitats. Its large number of two componenent systems are probably critical to its ability to sense and respond to its environment. Our goal is to map these RRs to the genes they regulate using a DNA-affinity-purification-chip (DAP-chip) protocol. First target determined usuing EMSA. A positive target was determined for as many RRs as possible using EMSA. Targets were selected based on gene proximity, regulon predictions and/or predicted sigma54 dependent promoters. qPCR was used to ensure that the target was enriched from sheared genomic DNA before proceeding to the DAP-chip.

  17. Volatile compounds of dry beans (Phaseolus vulgaris L.).

    Science.gov (United States)

    Oomah, B Dave; Liang, Lisa S Y; Balasubramanian, Parthiba

    2007-12-01

    Volatile compounds of uncooked dry bean (Phaseolus vulgaris L.) cultivars representing three market classes (black, dark red kidney and pinto) grown in 2005 were isolated with headspace solid phase microextraction (HS-SPME), and analyzed with gas chromatography mass spectrometry (GC-MS). A total of 62 volatiles consisting of aromatic hydrocarbons, aldehydes, alkanes, alcohols and ketones represented on average 62, 38, 21, 12, and 9 x 10(6) total area counts, respectively. Bean cultivars differed in abundance and profile of volatiles. The combination of 18 compounds comprising a common profile explained 79% of the variance among cultivars based on principal component analysis (PCA). The SPME technique proved to be a rapid and effective method for routine evaluation of dry bean volatile profile.

  18. Nootropic Effects of Filipendula Vulgaris Moench Water Extract Fractions.

    Science.gov (United States)

    Shilova, I V; Suslov, N I; Amelchenko, V P

    2015-07-01

    Nootropic activity of water extract fractions from aerial parts of Filipendula vulgaris Moench was demonstrated on the models of hermetic volume hypoxia, conditioned passive avoidance response, open field test, and forced swimming with a load. The fractions stimulated hypoxic resistance, normalized orientation and exploratory behavior, improved conditioned response reproduction during testing after hypoxic injury, and increased exercise tolerance. Fractionation of the extract led to dissociation of the effect components, which suggests that individual constituents have specific characteristics. Ethylacetate fraction exhibited most pronounced nootropic activity and was superior to plant extract by some characteristics. The detected effects seemed to be caused by modulation of the hippocampus activity the under the effects of phenol and triterpene compounds.

  19. Injury response of Phaseolus vulgaris to ozone flux density

    Science.gov (United States)

    Amiro, B. D.; Gillespie, T. J.; Thurtell, G. W.

    This study describes a quantitative relationship between mean O 3 flux density and the length of exposure needed for the occurrence of visual injury to Phaseolus vulgaris L. Similar relationships were found for 14 day old and 6 week old plants using a whole leaf gas exchange cuvette system. Cultivars Seafarer (O 3 sensitive) and Gold Crop (O 3 resistant) exhibited similar responses at flux densities > 3 mg m -2 h -1 but only Seafarer was injured below this flux density. O 3 concentration and length of exposure period alone did not contain sufficient information to describe the onset of visual foliar injury. The use of O 3 concentrations in excess of normal ambient conditions compensated for low leaf conductances so that flux densities in the cuvette were similar to those found in the field.

  20. Lichen striatus associated with psoriasis vulgaris treated with oral acitretin

    Directory of Open Access Journals (Sweden)

    Enzo Errichetti

    2014-01-01

    Full Text Available Lichen striatus (LS is an uncommon dermatosis of unknown etiology that presents as a continuous or interrupted linear band of pink, tan, red or skin-colored papules in a blaschkoid distribution. The lesions are generally solitary and unilateral, but unusual extensive cases with multiple and bilateral lesions have been also described. Albeit LS is typically an asymptomatic and self-limited dermatosis, it may cause a significant psychological distress in some patients, thus requiring an appropriate therapy. Topical steroid is the most commonly used treatment but it is not always effective. We report a case of LS unresponsive to topical steroid therapy associated with psoriasis vulgaris successfully treated with oral acitretin.

  1. PRODUCTIVITY OF MICROALGAE CHLORELLA VULGARIS IN LABORATORY CONDITION

    Directory of Open Access Journals (Sweden)

    Agnieszka Patyna

    2017-06-01

    Full Text Available Algae biomass is increasingly regarded as a potential resource that could be used to produce biofuels, electricity and heat. Algae contain a lot of nutrients, so they can be used as food for humans and livestock. Because of their valuable composition (many nutrients they are used as supplements of balanced diet, in turn taking into account their biosorption abbility they are used to detoxification of human body. Algae cultivation does not demand large areas of land to expose cells to sunlight, so their production rate is higher than vascular plants. Moreover algae cultivation lets to achieve high biomass concentration. Important cultivation factors are: illumination (light intensity is an important factor because it drives photosynthesis, CO2 supply, culture medium and mixing. The experimental research was conducted using Chlorella vulgaris BA 002 strain. The aim of this study was to determine the effectiveness of biomass growth in laboratory condition.

  2. Thermogravimetric analysis of the gasification of microalgae Chlorella vulgaris.

    Science.gov (United States)

    Figueira, Camila Emilia; Moreira, Paulo Firmino; Giudici, Reinaldo

    2015-12-01

    The gasification of microalgae Chlorella vulgaris under an atmosphere of argon and water vapor was investigated by thermogravimetric analysis. The data were interpreted by using conventional isoconversional methods and also by the independent parallel reaction (IPR) model, in which the degradation is considered to happen individually to each pseudo-component of biomass (lipid, carbohydrate and protein). The IPR model allows obtaining the kinetic parameters of the degradation reaction of each component. Three main stages were observed during the gasification process and the differential thermogravimetric curve was satisfactorily fitted by the IPR model considering three pseudocomponents. The comparison of the activation energy values obtained by the methods and those found in the literature for other microalgae was satisfactory. Quantification of reaction products was performed using online gas chromatography. The major products detected were H2, CO and CH4, indicating the potential for producing fuel gas and syngas from microalgae.

  3. Oral pemphigus vulgaris: A case report with direct immunofluorescence study

    Science.gov (United States)

    Kumar, Sangeetha Jeevan; Nehru Anand, SP; Gunasekaran, Nandhini; Krishnan, Rajkumar

    2016-01-01

    Pemphigus vulgaris (PV) is a chronic, autoimmune, intraepidermal blistering disease of the skin and mucous membranes. The initial clinical manifestation is frequently the development of intraoral lesions, and later, the lesions involve the other mucous membranes and skin. The etiology of this disease still remains obscure although the presence of autoantibodies is consistent with an autoimmune disease. These antibodies are targeted against the adhesion proteins of keratinocytes, leading to acantholysis (disruption of spinous layer, leading to intraepidermal clefting) and blister formation. Because only oral lesions are present initially, the chances of misdiagnosing the disease as another condition are increased, leading to inappropriate therapy. In this article, we report a case of PV with only oral manifestations in a 36-year-old male. PMID:27721634

  4. Four cases of recalcitrant pemphigus vulgaris salvaged with rituximab

    Directory of Open Access Journals (Sweden)

    Samyak Ganjre

    2017-01-01

    Full Text Available Although the long-term use of immunosuppressives – supplemented with more aggressive treatments such as immunoadsorption, intravenous immunoglobulins, or plasmapheresis in recalcitrant cases has dramatically improved the prognosis of pemphigus vulgaris, opportunistic infections secondary to immunosuppression continue to cause significant mortality. We report four cases– three old ones, who had accumulated significant morbidities over their disease duration ranging from 5 to 10 years, and the fourth, a teenage female intolerant to corticosteroids and idiosyncratic to methotrexate– who achieved complete remission on administration of rituximab by the lymphoma protocol. One of the old cases who had recalcitrant mucositis experienced its complete subsidence without any adjuvant whatsoever. All continue to remain asymptomatic for 11–20 months. None had infusion reactions or any delayed side effects.

  5. Future therapies for pemphigus vulgaris: Rituximab and beyond.

    Science.gov (United States)

    Huang, Amy; Madan, Raman K; Levitt, Jacob

    2016-04-01

    The conventional treatment for patients with pemphigus vulgaris (PV) centers on global immunosuppression, such as the use of steroids and other immunosuppressive drugs, to decrease titers of antidesmoglein autoantibodies responsible for the acantholytic blisters. Global immunosuppressants, however, cause serious side effects. The emergence of anti-CD20 biologic medications, such as rituximab, as an adjunct to conventional therapy has shifted the focus to targeted destruction of autoimmune B cells. Next-generation biologic medications with improved modes of delivery, pharmacology, and side effect profiles are constantly being developed, adding to the diversity of options for PV treatment. We review promising monoclonal antibodies, including veltuzumab, obinutuzumab (GA-101), ofatumumab, ocaratuzumab (AME-133v), PRO131921, and belimumab.

  6. Uric acid : A new antioxidant in patients with pemphigus vulgaris

    Directory of Open Access Journals (Sweden)

    Maryam Yousefi

    2011-01-01

    Full Text Available Background: Increased reactive oxygen species (ROS and lipid peroxidation are seen in many dermatologic disorders, for example, atopic dermatitis, psoriasis, vitiligo, acne vulgaris, pemphigus vulgaris (PV, lichen planus, and alopecia areata. ROS has an important role in the inflammation process. In PV, increased production of ROS leads to decline of antioxidants in plasma and red blood cells which results in oxidative stress. We aimed to evaluate the level of these antioxidants in PV patients and compare it to the controls. Materials and Methods: Among patients attending the dermatology clinics, 30 patients with PV, who had never been on treatment, were enrolled to the study. The control group consisted of 30 age- and sex-matched healthy non-smoker individuals. Venous blood was collected from the subjects for the evaluation of plasma levels of glutathione peroxidase, vitamin C, selenium, bilirubin, and uric acid. Results: Age mean and standard deviation of the patients (40.83, 12.74 was comparable to the controls (41.96, 13.08. Mean level of uric acid was significantly lower in PV patients compared to the controls (P = 0.006. Other antioxidants were not different between the two groups. Uric acid of the patients with mucosal involvement was significantly lower than patients with mucocutaneous involvement (P = 0.049. Limitations: The blood level of other antioxidants (e.g. malondialdehyde was not evaluated. Conclusions: Uric acid as an antioxidant in our study had similar changes to previous studies in the field of other diseases but selenium, bilirubin, and glutathione peroxidase did not differ between patients and controls.

  7. Clonal propagation of Bambusa vulgaris by leafy branch cuttings

    Institute of Scientific and Technical Information of China (English)

    M.S.Islam; M.K.Bhuiyan; M.M.Hossain; M.A.Hossain

    2011-01-01

    Bambusa vulgaris Schrad ex wendl is a widely cultivated bamboo species in rural Bangladesh for its versatile uses. The vegetative propagation becomes the only viable alternative for this species because B. vulgaris does not set seed after sparse flowering, which makes seed- ling progenies unavailable. A low-cost propagation trial was conducted to explore the clonal propagation techniques for the species with two types of small branch cuttings, nodal leafy cuttings and tip cuttings. The cuttings, after treating with 0, 0.1%, 0.4%, and 0.8% IBA solutions, were kept in non-mist propagator to let them to root for assessing the rooting ability. The cuttings were rooted in four weeks and were allowed to grow in the polybags for 10 months under nursery condition to assess their steckling capacity. The study reveals that both types of branch cuttings are able to develop roots, shoots, to survive and to form rhizome under the nursery condition. Rooting ability of the cuttings was significantly enhanced by the application of rooting hormone - IBA. The highest root- ing percentage in nodal leafy cuttings and the tip cuttings (56.67% and 51.0%, respectively) were observed in 0.8% IBA treatment, followed by 0.4% IBA and the lowest (34.3% and 30.0%, respectively) was in control. The highest number of root developed per cutting (9.77 and 8.33 in nodal leafy cuttings and the tip cuttings, respectively) was also obtained from the cuttings treated with 0.8% IBA solution, followed 0.4% IBA treat- ment and the lowest (3.1 and 2.1, respectively) was in the cuttings with- out treatment. However, the length of the longest root varied significantly neither with the cutting types nor the concentrations of IBA solution. Survival percentage of the stecklings in nursery condition was signifi- cantly enhanced by IBA.

  8. Transcriptome Profiling of the Phaseolus vulgaris - Colletotrichum lindemuthianum Pathosystem.

    Science.gov (United States)

    Padder, Bilal A; Kamfwa, Kelvin; Awale, Halima E; Kelly, James D

    2016-01-01

    Bean (Phaseolus vulgaris) anthracnose caused by the hemi-biotrophic pathogen Colletotrichum lindemuthianum is a major factor limiting production worldwide. Although sources of resistance have been identified and characterized, the early molecular events in the host-pathogen interface have not been investigated. In the current study, we conducted a comprehensive transcriptome analysis using Illumina sequencing of two near isogenic lines (NILs) differing for the presence of the Co-1 gene on chromosome Pv01 during a time course following infection with race 73 of C. lindemuthianum. From this, we identified 3,250 significantly differentially expressed genes (DEGs) within and between the NILs over the time course of infection. During the biotrophic phase the majority of DEGs were up regulated in the susceptible NIL, whereas more DEGs were up-regulated in the resistant NIL during the necrotrophic phase. Various defense related genes, such as those encoding PR proteins, peroxidases, lipoxygenases were up regulated in the resistant NIL. Conversely, genes encoding sugar transporters were up-regulated in the susceptible NIL during the later stages of infection. Additionally, numerous transcription factors (TFs) and candidate genes within the vicinity of the Co-1 locus were differentially expressed, suggesting a global reprogramming of gene expression in and around the Co-1 locus. Through this analysis, we reduced the previous number of candidate genes reported at the Co-1 locus from eight to three. These results suggest the dynamic nature of P. vulgaris-C. lindemuthianum interaction at the transcriptomic level and reflect the role of both pathogen and effector triggered immunity on changes in plant gene expression.

  9. Global Analysis of Heat Shock Response in Desulfovibrio vulgaris Hildenborough.

    Energy Technology Data Exchange (ETDEWEB)

    Chhabra, S.R.; He, Q.; Huang, K.H.; Gaucher, S.P.; Alm, E.J.; He,Z.; Hadi, M.Z.; Hazen, T.C.; Wall, J.D.; Zhou, J.; Arkin, A.P.; Singh, A.K.

    2005-09-16

    Desulfovibrio vulgaris Hildenborough belongs to a class ofsulfate-reducing bacteria (SRB) and is found ubiquitously in nature.Given the importance of SRB-mediated reduction for bioremediation ofmetal ion contaminants, ongoing research on D. vulgaris has been in thedirection of elucidating regulatory mechanisms for this organism under avariety of stress conditions. This work presents a global view of thisorganism's response to elevated growth temperature using whole-celltranscriptomics and proteomics tools. Transcriptional response (1.7-foldchange or greater; Z>1.5) ranged from 1,135 genes at 15 min to 1,463genes at 120 min for a temperature up-shift of 13oC from a growthtemperature of 37oC for this organism and suggested both direct andindirect modes of heat sensing. Clusters of orthologous group categoriesthat were significantly affected included posttranslationalmodifications; protein turnover and chaperones (up-regulated); energyproduction and conversion (down-regulated), nucleotide transport,metabolism (down-regulated), and translation; ribosomal structure; andbiogenesis (down-regulated). Analysis of the genome sequence revealed thepresence of features of both negative and positive regulation whichincluded the CIRCE element and promoter sequences corresponding to thealternate sigma factors ?32 and ?54. While mechanisms of heat shockcontrol for some genes appeared to coincide with those established forEscherichia coli and Bacillus subtilis, the presence of unique controlschemes for several other genes was also evident. Analysis of proteinexpression levels using differential in-gel electrophoresis suggestedgood agreement with transcriptional profiles of several heat shockproteins, including DnaK (DVU0811), HtpG (DVU2643), HtrA (DVU1468), andAhpC (DVU2247). The proteomics study also suggested the possibility ofposttranslational modifications in the chaperones DnaK, AhpC, GroES(DVU1977), and GroEL (DVU1976) and also several periplasmic ABCtransporters.

  10. Energy metabolism in Desulfovibrio vulgaris Hildenborough: insights from transcriptome analysis

    Energy Technology Data Exchange (ETDEWEB)

    Pereira, Patricia M.; He, Qiang; Valente, Filipa M.A.; Xavier, Antonio V.; Zhou, Jizhong; Pereira, Ines A.C.; Louro, Ricardo O.

    2007-11-01

    Sulphate-reducing bacteria are important players in the global sulphur and carbon cycles, with considerable economical and ecological impact. However, the process of sulphate respiration is still incompletely understood. Several mechanisms of energy conservation have been proposed, but it is unclear how the different strategies contribute to the overall process. In order to obtain a deeper insight into the energy metabolism of sulphate-reducers whole-genome microarrays were used to compare the transcriptional response of Desulfovibrio vulgaris Hildenborough grown with hydrogen/sulphate, pyruvate/sulphate, pyruvate with limiting sulphate, and lactate/thiosulphate, relative to growth in lactate/sulphate. Growth with hydrogen/sulphate showed the largest number of differentially expressed genes and the largest changes in transcript levels. In this condition the most up-regulated energy metabolism genes were those coding for the periplasmic [NiFeSe]hydrogenase, followed by the Ech hydrogenase. The results also provide evidence for the involvement of formate cycling and the recently proposed ethanol pathway during growth in hydrogen. The pathway involving CO cycling is relevant during growth on lactate and pyruvate, but not during growth in hydrogen as the most down-regulated genes were those coding for the CO-induced hydrogenase. Growth on lactate/thiosulphate reveals a down-regulation of several energymetabolism genes similar to what was observed in the presence of nitrite. This study identifies the role of several proteins involved in the energy metabolism of D. vulgaris and highlights several novel genes related to this process, revealing a more complex bioenergetic metabolism than previously considered.

  11. Enzymatic and non-enzymatic antioxidant potentials of Chlorella vulgaris grown in effluent of a confectionery industry

    OpenAIRE

    2011-01-01

    Enzymatic and non-enzymatic antioxidant potentials of Chlorella vulgaris have gained considerable importance in recent decades. C. vulgaris strain highly tolerant to extreme pH variations was isolated and mass-cultivated in the wastewater from a confectionery industry. C.vulgaris showed better growth in wastewater than in improvised CFTRI medium. The microalgal biomass was then screened for the following antioxidants: peroxidase, superoxide dismutase, polyphenol oxidase, glutathione peroxidas...

  12. Hubungan Stres dengan Kejadian Akne Vulgaris Di kalangan Mahasiswa Fakultas Kedokteran Universitas Sumatera Utara Angkatan 2007-2009

    OpenAIRE

    Perumal, Nitya

    2011-01-01

    Background: Acne or acne vulgaris has always been well concerned among teenagers and adolescent age. Acne vulgaris is a common disorder of the follicle sebaceous unit that predominantly occurs in adolescence. Acne vulgaris which is suffered by 95 to 100 percent boys and 83 to 85 percent girls. This skin disease is caused by multifactoral of skin disease, which is stress. With the increased studying burden, packed time table and inadequate sleep has caused medical students in University of Nor...

  13. Hepatoprotective Effect of Pretreatment with Thymus vulgaris Essential Oil in Experimental Model of Acetaminophen-Induced Injury

    National Research Council Canada - National Science Library

    Grespan, Renata; Aguiar, Rafael Pazinatto; Giubilei, Frederico Nunes; Fuso, Rafael Rocco; Damião, Marcio José; Silva, Expedito Leite; Mikcha, Jane Graton; Hernandes, Luzmarina; Bersani Amado, Ciomar; Cuman, Roberto Kenji Nakamura

    2014-01-01

    .... This study investigated the hepatoprotective effect of Thymus vulgaris essential oil (TEO), which is used popularly for various beneficial effects, such as its antiseptic, carminative, and antimicrobial effects...

  14. A Comparative Effectiveness Research of Azathioprine and Cyclophosphamide on the Clinical and Serological Response in Pemphigus Vulgaris

    Science.gov (United States)

    Sardana, Kabir; Agarwal, Pooja; Bansal, Shivani; Uppal, Beena; Garg, Vijay K

    2016-01-01

    Context: A prospective study was carried out to examine the efficacy of cyclophosphamide and azathioprine in pemphigus vulgaris. Aims: To compare the clinical and serological effect of azathioprine and cyclophosphamide in pemphigus patients. Materials and Methods: Prospective, institutional based study was conducted twenty-one patients of pemphigus vulgaris were initiated on either azathioprine (n = 9) or cyclophosphamide (n = 7) in addition to prednisolone and were evaluated clinically (mucosal and cutaneous severity) and serologically enzyme-linked immunosorbent assay (ELISA) at 0, 3 and 6 months. Results: Azathioprine had a slower onset of action with a statistically significant improvement seen by 6 months (P = 0.016). Cyclophosphamide had a faster onset of action (3 months) though there was no statistical difference in the efficacy between the two at the end of 6 months. The (RonT) was 33.3–44.4% for azathioprine and 28.8–42.9% for cyclophosphamide at 6 months. Though ELISA had a high sensitivity and specificity for diagnosis, as a tool for assessing therapeutic response a significant decrease was seen only till 3 months. This was restricted to Dsg1 for the azathioprine group and both Dsg3 and Dsg1 levels for the cyclophosphamide group. There were two deaths, both in the cyclophosphamide group. Conclusions: Azathiorpine and cyclophosphamide are equally effective for mucosal and cutaneous disease in pemphigus after 6 months of therapy. Dsg ELISA is useful for diagnosis of pemphigus but is not a useful tool for monitoring response to therapy. PMID:27512188

  15. Uranium and cesium accumulation in bean (Phaseolus vulgaris L. var. vulgaris) and its potential for uranium rhizofiltration.

    Science.gov (United States)

    Yang, Minjune; Jawitz, James W; Lee, Minhee

    2015-02-01

    Laboratory scale rhizofiltration experiments were performed to investigate uranium and cesium accumulation in bean (Phaseolus vulgaris L. var. vulgaris) and its potential for treatment of uranium contaminated groundwater. During 72 h of rhizofiltration, the roots of the bean accumulated uranium and cesium to concentrations 317-1019 times above the initial concentrations, which ranged from 100 to 700 μg l(-1) in artificially contaminated solutions. When the pH of the solution was adjusted to 3, the ability to accumulate uranium was 1.6 times higher than it was for solutions of pH 7 and pH 9. With an initial uranium concentration of 240 μg l(-1) in genuine groundwater at pH 5, the bean reduced the uranium concentration by 90.2% (to 23.6 μg l(-1)) within 12 h and by 98.9% (to 2.8 μg l(-1)) within 72 h. A laboratory scale continuous clean-up system reduced uranium concentrations from 240 μg l(-1) to below 10 μg l(-1) in 56 h; the whole uranium concentration in the bean roots during system operation was more than 2600 μg g(-1) on a dry weight basis. Using SEM and EDS analyses, the uranium removal in solution at pH 7 was determined based on adsorption and precipitation on the root surface in the form of insoluble uranium compounds. The present results demonstrate that the rhizofiltration technique using beans efficiently removes uranium and cesium from groundwater as an eco-friendly and cost-effective method.

  16. Effect of starvation stress on morphological changes and production of adhesive exopolysaccharide (EPS by Proteus vulgaris

    Directory of Open Access Journals (Sweden)

    Kamila Myszka

    2011-09-01

    Full Text Available   Background. Proteus vulgaris attach to available surfaces in industrial environments, can develop into extensive biofilm. Such bacterial layer is a potential source of contamination of foods that may lead to spoilage or transmission foodborne pathogens. The purpose of these investigations was to evaluate the influence of limited nutrients availability in the medium on the morphological changes and biosynthesis of bacterial surface-associated EPS by P. vulgaris. The relationship between the dimension of cells, EPS production and P. vulgaris biofilm development process on stainless steel surfaces (type 316L was also examined. Material and methods. P. vulgaris ATCC 6380 was used in this study. The cultures were incubated at 37°C on the Enterobacteriaceae enrichment broth according to Mossel [1962]. During the investigations the medium with optimal and 10 times diluted optimal of nutrient availability were used. For cells dimension analysis a Carl-Zeiss Axiovert 200 inverted microscope and a scanning electron microscope (LEO 435VP was applied. Isolation of exopolysaccharides was based on the procedure employed by Forde and Fitzgerald [1999]. To determine the level of P. vulgaris adhesion to the surface of stainless steel, the method described by Le Thi et al. [2001] was used. Results. In all experimental variants the area of P. vulgaris cells was changed upon long-term starvation. Altering of physical dimension of bacteria was effected by the decreasing value of the cell length. The change of P. vulgaris morphology promoted the beginning stages of biofilm formation process on the surface of stainless steel. Under starvation conditions P. vulgaris produced more EPS. It was observed with an increase of incubation period. These extracellular molecules initiated more advanced stages of P. vulgaris biofilm formation on examined surfaces. Conclusion. The data support the notion thatcellular factors influencing P. vulgaris adhesion process to abiotic

  17. XML Investigating the Phytochemical, Antibacterial and Antifungal Effects of Thymus Vulgaris and Cuminum Cyminum Essential Oils

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    Soghra Valizadeh (MSc

    2016-02-01

    Full Text Available Background and Objective: The aim of this study was to determine the chemical composition, antibacterial and antifungal effects of Thymus vulgaris and Cuminum Cyminum essential oils against foodborne pathogens and Candida species in vitro. Methods: The essential oils were extracted from the aerial parts of Thymus vulgaris and dried Cuminum Cyminum seeds using a Clevenger apparatus for 3 hours. Analysis of the essential oils’ constituents was performed using gas chromatography-mass spectrophotometry. The antibacterial activity of Cuminum Cyminum essential oil and essential oil of Thymus vulgaris against Bacillus cereus, Listeria monocytogenes, Escherichia coli and Salmonella typhimurium were evaluated in agar culture medium. The minimum inhibitory concentration (MIC of these essential oils against fungal strains of Candida albicans, C. tropicalis, C. parapsilosis and C. dubliniensis was measured. Results: Thymol (64.45% and cuminaldehyde (29.02% were the main components of the essential oil of Thymus vulgaris and Cuminum Cyminum, respectively. The largest inhibition zone diameter in the essential oils of Thymus vulgaris and Cuminum Cyminum in the agar disk diffusion method was related to B. cereus with 30 and 21 mm diameter, respectively. The largest growth inhibition zone diameter by the essential oil of Thymus vulgaris in the well diffusion method was 21 mm and against B. cereus. The MIC of essential oil of Thymus vulgaris in the microdilution method was 0.09% against all the four Candida strains. The MIC of Cuminum Cyminum essential oil against strains of C. albicans and C. tropicalis was 0.39%, while it was found as 0.19% against C. parapsilosis and C. dubliniensis. Conclusion: In this study, Cuminum Cyminum essential oil and essential oil of Thymus vulgaris show suitable inhibitory effects against the growth of bacteria using well and disk diffusion methods. Regarding the antifungal effects, the MIC of essential oil of Thymus vulgaris is

  18. Efficient Approaches for Designing Fault Tolerant Reversible Carry Look-Ahead and Carry-Skip Adders

    CERN Document Server

    Islam, Md Saiful; begum, Zerina; Hafiz, Mohd Zulfiquar

    2010-01-01

    Combinational or Classical logic circuits dissipate heat for every bit of information that is lost. Information is lost when the input vector cannot be recovered from its corresponding output vector. Reversible logic circuit implements only the functions having one-to-one mapping between its input and output vectors and therefore naturally takes care of heating. Reversible logic design becomes one of the promising research directions in low power dissipating circuit design in the past few years and has found its application in low power CMOS design, digital signal processing and nanotechnology. This paper presents the efficient approaches for designing fault tolerant reversible fast adders that implement carry look-ahead and carry-skip logic. The proposed high speed reversible adders include MIG gates for the realization of its basic building block. The MIG gate is universal and parity preserving. It allows any fault that affects no more than a single signal readily detectable at the circuit's primary outputs...

  19. A meta-analysis of association between acne vulgaris and Demodex infestation

    Institute of Scientific and Technical Information of China (English)

    Ya-e ZHAO; Li HU; Li-ping WU; Jun-xian MA

    2012-01-01

    Until now,etiology of acne vulgaris is still uncertain.Although clinicians usually deny the association between Demodex infestation and acne vulgaris,it has been proved in some clinical practices.To confirm the association between Demodex infestation and acne vulgaris,a meta-analysis was conducted.Predefined selection criteria were applied to search all published papers that analyzed the association between Demodex infestation and acne vulgaris (January 1950 to August 2011) in ISl Web of Knowledge,MEDLINE,and China National Knowledge Infrastructure (CNKI) databases.A meta-analysis was performed to calculate odds ratios (ORs) and 95% confidence intervals (CIs)based on fixed effects models or random effects models.We enrolled the 60 Chinese and 3 English papers in this meta-analysis,which covered Turkey and 25 different provinces/municipalities in China and 42130 participants including students and residents,aged from 1 to 78 years.The pooled OR in random effects models is 2.80 (95% Cl,2.34-3.36).Stability is robust according to sensitivity analysis.The fail-safe number is 18477,suggesting that at least 18477 articles with negative conclusions would be needed to reverse the conclusion that acne vulgaris was related to Demodex infestation.So the effect of publication bias was insignificant and could be ignored.It was concluded that acne vulgaris is associated with Demodex infestation.This indicates that when regular treatments for acne vulgaris are ineffective,examination of Demodex mites and necessary acaricidal therapies should be considered.

  20. Decreased eicosapentaenoic acid levels in acne vulgaris reveals the presence of a proinflammatory state.

    Science.gov (United States)

    Aslan, İbrahim; Özcan, Filiz; Karaarslan, Taner; Kıraç, Ebru; Aslan, Mutay

    2017-01-01

    This study aimed to determine circulating levels of polyunsaturated fatty acids (PUFAs), secretory phospholipase A2 (sPLA2), lipoprotein lipase (LPL) and measure circulating protein levels of angiopoietin-like protein 3 (ANGPTL3), ANGPTL4, cyclooxygenase-2 (COX-2) and prostaglandin E2 (PGE2) in patients with acne vulgaris. Serum from 21 control subjects and 31 acne vulgaris patients were evaluated for levels of arachidonic acid (AA, C20:4n- 6), dihomo-gamma-linolenic acid (DGLA, C20:3n-6), eicosapentaenoic acid (EPA, C20:5n-3) and docosahexaenoic acid (DHA, C22:6n-3). PUFA levels were determined by an optimized multiple reaction monitoring (MRM) method using ultra fast-liquid chromatography (UFLC) coupled with tandem mass spectrometry (MS/MS). Lipid profile, routine biochemical and hormone parameters were assayed by standard kit methods Serum EPA levels were significantly decreased while AA/EPA and DGLA/EPA ratio were significantly increased in acne vulgaris patients compared to controls. Serum levels of AA, DGLA and DHA showed no significant difference while activity of sPLA2 and LPL were significantly increased in acne vulgaris compared to controls. Results of this study reveal the presence of a proinflammatory state in acne vulgaris as shown by significantly decreased serum EPA levels and increased activity of sPLA2, AA/EPA and DGLA/EPA ratio. Increased LPL activity in the serum of acne vulgaris patients can be protective through its anti-dyslipidemic actions. This is the first study reporting altered EPA levels and increased sPLA2 activity in acne vulgaris and supports the use of omega-3 fatty acids as adjuvant treatment for acne patients.

  1. Carum copticum and Thymus vulgaris oils inhibit virulence in Trichophyton rubrum and Aspergillus spp

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    Mohd Sajjad Ahmad Khan

    2014-06-01

    Full Text Available Emergence of drug-resistant strains has demanded for alternative means of combating fungal infections. Oils of Carum copticum and Thymus vulgaris have long been used in ethnomedicine for ailments of various fungal infections. Since their activity has not been reported in particular against drug-resistant fungi, this study was aimed to evaluate the effects of oils of C. copticum and T. vulgaris on the growth and virulence of drug-resistant strains of Aspergillus spp. and Trichophyton rubrum. The gas chromatography-mass spectrometry analysis revealed thymol constituting 44.71% and 22.82% of T. vulgaris and C. copticum, respectively. Inhibition of mycelial growth by essential oils was recorded in the order of thymol > T. vulgaris > C. copticum against the tested strains. RBC lysis assay showed no tested oils to be toxic even up to concentration two folds higher than their respective MFCs. Thymol exhibited highest synergy in combination with fluconazole against Aspergillus fumigatus MTCC2550 (FICI value 0.187 and T. rubrum IOA9 (0.156 as determined by checkerboard method. Thymol and T. vulgaris essential oil were equally effective against both the macro and arthroconidia growth (MIC 72 µg/mL. A > 80% reduction in elastase activity was recorded for A. fumigatus MTCC2550 by C. copticum, T. vulgaris oils and thymol. The effectiveness of these oils against arthroconidia and synergistic interaction of thymol and T. vulgaris with fluconazole can be exploited to potentiate the antifungal effects of fluconazole against drug-resistant strains of T. rubrum and Aspergillus spp.

  2. Carum copticum and Thymus vulgaris oils inhibit virulence in Trichophyton rubrum and Aspergillus spp.

    Science.gov (United States)

    Khan, Mohd Sajjad Ahmad; Ahmad, Iqbal; Cameotra, Swaranjit Singh

    2014-01-01

    Emergence of drug-resistant strains has demanded for alternative means of combating fungal infections. Oils of Carum copticum and Thymus vulgaris have long been used in ethnomedicine for ailments of various fungal infections. Since their activity has not been reported in particular against drug-resistant fungi, this study was aimed to evaluate the effects of oils of C. copticum and T. vulgaris on the growth and virulence of drug-resistant strains of Aspergillus spp. and Trichophyton rubrum. The gas chromatography-mass spectrometry analysis revealed thymol constituting 44.71% and 22.82% of T. vulgaris and C. copticum, respectively. Inhibition of mycelial growth by essential oils was recorded in the order of thymol > T. vulgaris > C. copticum against the tested strains. RBC lysis assay showed no tested oils to be toxic even up to concentration two folds higher than their respective MFCs. Thymol exhibited highest synergy in combination with fluconazole against Aspergillus fumigatus MTCC2550 (FICI value 0.187) and T. rubrum IOA9 (0.156) as determined by checkerboard method. Thymol and T. vulgaris essential oil were equally effective against both the macro and arthroconidia growth (MIC 72 μg/mL). A > 80% reduction in elastase activity was recorded for A. fumigatus MTCC2550 by C. copticum, T. vulgaris oils and thymol. The effectiveness of these oils against arthroconidia and synergistic interaction of thymol and T. vulgaris with fluconazole can be exploited to potentiate the antifungal effects of fluconazole against drug-resistant strains of T. rubrum and Aspergillus spp.

  3. Antioxidant activity of the microalga Chlorella vulgaris cultered on special conditions.

    Science.gov (United States)

    Miranda, M S; Sato, S; Mancini-Filho, J

    2001-01-01

    The chemical composition of Chlorella vulgaris indicates that it has a high nutritional value to a wide range of essential nutrients, such as vitamins, minerals and proteins. Moreover, it contains other compounds such as n-3 and n-6 polynsaturated fatty acids, provitamins and phenolic compounds. In addition, this alga can be produced in large-scale systems. The objective of the present study was to evaluate the antioxidant capacity of a Chlorella cultured on three differents temperatures (15 degrees C, 20 degrees C and 30 degrees C) in 3 Klux. Chlorella cultured samples were submitted to sequential extration using as solvents: ether, methanol and water. The antioxidant activity in the extracts was measured by b-carotene/linoleic acid system, at 50 degrees C and absorbances reading at 470 nm. One control with BHT, 100 ppm was used in this determination. The total phenolic compounds was determined with Folin-Ciocalteu reagent using the spectrophotometric measured at 780 nm with catechin as standard. The phenolic acid analysis were carried out using gas chromatograph equipped with a capillary column and flame ionization detector. Non conjugated and total phenolic acids were identified on the basis of the relative retention time of their derivatives compared with the standard phenolic acids. The methanolic extract from Chlorella cultured at 30 degrees C showed higher antioxidant activity (85%) quite similar of BHT (86%). By the Rancimat test (lipidic medium) two fractions from methanolic extracts showed too higher antioxidant activity with induction times > 37.50 h at 60 degrees C and 11.5 h at 100 degrees C. The total phenolic compounds were 24.95 mg in 1 g of dry alga matter from methanolic extract and five phenolic acids were identified. The phenolic compounds salicylic, trans cinnamic, synaptic, chlorogenic, chimic and caffeic acids found in the methanolic Chlorella extract may be responsible for its higher antioxidant activity.

  4. Biochemistry and cell ultrastructure changes during senescence of Beta vulgaris L. leaf.

    Science.gov (United States)

    Romanova, Alla K; Semenova, Galina A; Ignat'ev, Alexander R; Novichkova, Natalia S; Fomina, Irina R

    2016-05-01

    The comparative study of biochemical and ultrastructure features in senescing sugar beet (Beta vulgaris L.) leaves was carried out. One group of plants was grown under normal conditions in washed river sand and poured in turn with nitrate-containing mineral solution or water (N plants). Another group of plants, after 1 month of normal growth, was further grown with nitrate omitted in the nutritive solution (defN plants). The starting point of normal leaf senescence in N plants was identified by the maximal content of soluble protein. Soluble carbohydrate pools were statistically constant in senescing N plants, whereas glucose pools varied noticeably. A decrease in the contents of soluble protein and chlorophyll (a + b) in the course of senescing was typical for N plant leaves. The cell membrane in N plant leaves remained mostly intact; the central vacuoles in the leaf cells were large, and their membranes remained intact. The chloroplasts and mitochondria in senescing N plant leaves became swollen. The vesicles that were present in the cytoplasm of N plant leaves were especially large in the oldest leaves. It was concluded that senescing of sugar beet leaves at sufficient nitrate nutrition occurs according to a "vacuolar" scenario. In the case of nitrate deficiency, the content of soluble carbohydrates in defN leaves first reached maximum and then decreased in older leaves; the protein and chlorophyll (a + b) contents were totally lower than those in normal leaves and continuously decreased during the experiments. Chloroplasts in mesophyll cells of defN plant leaves became more rounded; starch grains in chloroplasts degraded and the number and size of lipid globules increased. The multitude of membrane impairments and lots of large vesicles-"crystals" appeared during the experiment. The results showed the controlling action of nitrogen nutrition in the senescing of sugar beet leaves.

  5. Evidence for paternal transmission and heteroplasmy in the mitochondrial genome of Silene vulgaris, a gynodioecious plant.

    Science.gov (United States)

    McCauley, D E; Bailey, M F; Sherman, N A; Darnell, M Z

    2005-07-01

    Gynodioecy refers to the co-occurrence of females and hermaphrodites in the same population. In many gynodioecious plants, sex is determined by an epistatic interaction between mitochondrial and nuclear genes, resulting in intragenomic evolutionary conflict, should the mitochondrial genome be maternally inherited. While maternal inheritance of the mitochondrial genome is common in angiosperms, few gynodioecious species have been studied. Here, the inheritance of the mitochondrial genes atpA and coxI was studied in 318 Silene vulgaris individuals distributed among 23 crosses. While maternal inheritance was indicated in 96% of the individuals studied, one or more individuals from each of four sib groups displayed a genotype that was identical to the father, or that did not match either parent. Given evidence that inheritance is not strictly maternal, it was hypothesized that some individuals could carry a mixture of maternally and paternally derived copies of the mitochondrial genome, a condition known as heteroplasmy. Since heteroplasmy might be difficult to detect should multiple versions of the mitochondrial genome co-occur in highly unequal copy number, a method was devised to amplify low-copy number forms of atpA differentially. Evidence for heteroplasmy was found in 23 of the 99 individuals studied, including cases in which the otherwise cryptic form of atpA matched the paternal genotype. The distribution of shared nucleotide sequence polymorphism among atpA haplotypes and the results of a population survey of the joint distribution of atpA and coxI haplotypes across individuals supports the hypothesis that heteroplasmy facilitates formation of novel mitochondrial genotypes by recombination.

  6. Filaggrin gene mutations in African Americans with both ichthyosis vulgaris and atopic dermatitis.

    Science.gov (United States)

    Polcari, Ingrid; Becker, Lauren; Stein, Sarah L; Smith, Marilyn S; Paller, Amy S

    2014-01-01

    Atopic dermatitis (AD) and ichthyosis vulgaris (IV) are two common disorders of epidermal homeostasis resulting in dry skin. The profilaggrin gene, located on chromosome 1q22, encodes a keratin filament aggregating protein (filaggrin) that is essential to forming the epidermal barrier and maintaining hydration. Null mutations in filaggrin have been found to underlie IV and are common in patients with AD, but the minority of African Americans with AD or IV show these mutations in filaggrin. We have selectively studied African Americans with both AD and IV to maximize the possibility of finding filaggrin null mutations in this population. DNA was collected using buccal swabs from 18 African American children with both AD and IV and 17 African American controls without either of these diseases. Purified genomic DNA was amplified using polymerase chain reaction from three regions of the filaggrin gene, exon 3, including R501X, 2282del4, E2554X, R2447X, 1249insG, R826X, 2767insT, and E2422X. Of the African American children with both AD and IV, 22.2% were heterozygous for filaggrin null mutations. Out of the control group, one carried a null mutation and was later discovered to have a history of asthma. Null mutations found in this population included R501X (n = 1), 2282del4 (n = 2), and R826X (n = 2, including the control patient). Our data demonstrate a prevalence of filaggrin mutations in the African American population that exceeds previously published data, although the overall prevalence is still lower than in other populations. It is likely that factors other than known FLG mutations are involved in African American patients.

  7. Proteomic definition of a desmoglein linear determinant common to Pemphigus vulgaris and Pemphigus foliaceous

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    Sinha Animesh A

    2006-08-01

    Full Text Available Abstract Background A number of autoimmune diseases have been clinically and pathologically characterized. In contrast, target antigens have been identified only in a few cases and, in these few cases, the knowledge of the exact epitopic antigenic sequence is still lacking. Thus the major objective of current work in the autoimmunity field is the identification of the epitopic sequences that are related to autoimmune reactions. Our labs propose that autoantigen peptide epitopes able to evoke humoral (autoimmune response are defined by the sequence similarity to the host proteome. The underlying scientific rationale is that antigen peptides acquire immunoreactivity in the context of their proteomic similarity level. Sequences uniquely owned by a protein will have high potential to evoke an immune reaction, whereas motifs with high proteomic redundancy should be immunogenically silenced by the tolerance phenomenon. The relationship between sequence redundancy and peptide immunoreactivity has been successfully validated in a number of experimental models. Here the hypothesis has been applied to pemphigus diseases and the corresponding desmoglein autoantigens. Methods Desmoglein 3 sequence similarity analysis to the human proteome followed by dot-blot/NMR immunoassays were carried out to identify and validate possible epitopic sequences. Results Computational analysis led to identifying a linear immunodominant desmoglein-3 epitope highly reactive with the sera from Pemphigus vulgaris as well as Pemphigus foliaceous. The epitopic peptide corresponded to the amino acid REWVKFAKPCRE sequence, was located in the extreme N-terminal region (residues 49 to 60, and had low redundancy to the human proteome. Sequence alignment showed that human desmoglein 1 and 3 share the REW-KFAK–RE sequence as a common motif with 75% residue identity. Conclusion This study 1 validates sequence redundancy to autoproteome as a main factor in shaping desmoglein peptide

  8. Global analysis of heat shock response in Desulfovibrio vulgaris Hildenborough.

    Energy Technology Data Exchange (ETDEWEB)

    Arkin, A. P. (Physical Biosciences Division, Berkeley, CA); Wall, J. D. (University of Missouri-Columbia, Columbia, MO); Hazen, T. C. (Physical Biosciences Division, Berkeley, CA); He, Z. (Oak Ridge National Laboratory, Oak Ridge, TN); Zhou, J. (Oak Ridge National Laboratory, Oak Ridge, TN); Huang, K. H. (Physical Biosciences Division, Berkeley, CA); Gaucher, Sara P.; He, Q. (Oak Ridge National Laboratory, Oak Ridge, TN); Hadi, Masood Z.; Chhabra, Swapnil R.; Alm, Eric J. (Physical Biosciences Division, Berkeley, CA); Singh, A. K.

    2005-08-01

    Desulfovibrio vulgaris Hildenborough belongs to a class of sulfate-reducing bacteria (SRB) and is found ubiquitously in nature. Given the importance of SRB-mediated reduction for bioremediation of metal ion contaminants, ongoing research on D. vulgaris has been in the direction of elucidating regulatory mechanisms for this organism under a variety of stress conditions. This work presents a global view of this organism's response to elevated growth temperature using whole-cell transcriptomics and proteomics tools. Transcriptional response (1.7-fold change or greater; Z {ge} 1.5) ranged from 1,135 genes at 15 min to 1,463 genes at 120 min for a temperature up-shift of 13 C from a growth temperature of 37 C for this organism and suggested both direct and indirect modes of heat sensing. Clusters of orthologous group categories that were significantly affected included posttranslational modifications; protein turnover and chaperones (up-regulated); energy production and conversion (down-regulated), nucleotide transport, metabolism (down-regulated), and translation; ribosomal structure; and biogenesis (down-regulated). Analysis of the genome sequence revealed the presence of features of both negative and positive regulation which included the CIRCE element and promoter sequences corresponding to the alternate sigma factors {sigma}{sup 32} and {sigma}{sup 54}. While mechanisms of heat shock control for some genes appeared to coincide with those established for Escherichia coli and Bacillus subtilis, the presence of unique control schemes for several other genes was also evident. Analysis of protein expression levels using differential in-gel electrophoresis suggested good agreement with transcriptional profiles of several heat shock proteins, including DnaK (DVU0811), HtpG (DVU2643), HtrA (DVU1468), and AhpC (DVU2247). The proteomics study also suggested the possibility of posttranslational modifications in the chaperones DnaK, AhpC, GroES (DVU1977), and GroEL (DVU

  9. Inhibition of lentivirus replication by aqueous extracts of Prunella vulgaris

    Directory of Open Access Journals (Sweden)

    Hauck Cathy

    2009-01-01

    Full Text Available Abstract Background Various members of the mint family have been used historically in Chinese and Native American medicine. Many of these same family members, including Prunella vulgaris, have been reported to have anti-viral activities. To further characterize the anti-lentiviral activities of P. vulgaris, water and ethanol extractions were tested for their ability to inhibit equine infectious anemia virus (EIAV replication. Results Aqueous extracts contained more anti-viral activity than did ethanol extracts, displaying potent anti-lentiviral activity against virus in cell lines as well as in primary cell cultures with little to no cellular cytotoxicity. Time-of-addition studies demonstrated that the extracts were effective when added during the first four h of the viral life cycle, suggesting that the botanical constituents were targeting the virion itself or early entry events. Further analysis revealed that the extracts did not destroy EIAV virion integrity, but prevented viral particles from binding to the surface of permissive cells. Modest levels of anti-EIAV activity were also detected when the cells were treated with the extracts prior to infection, indicating that anti-EIAV botanical constituents could interact with both viral particles and permissive cells to interfere with infectivity. Size fractionation of the extract demonstrated that eight of the nine fractions generated from aqueous extracts displayed anti-viral activity. Separation of ethanol soluble and insoluble compounds in the eight active fractions revealed that ethanol-soluble constituents were responsible for the anti-viral activity in one fraction whereas ethanol-insoluble constituents were important for the anti-viral activity in two of the other fractions. In three of the five fractions that lost activity upon sub-fractionation, anti-viral activity was restored upon reconstitution of the fractions, indicating that synergistic anti-viral activity is present in several

  10. Fault tolerant reversible logic synthesis: Carry look-ahead and carry-skip adders

    CERN Document Server

    Islam, Md Saiful; Begum, Zerina; Hafiz, Mohd Zulfiquar; 10.1109/ACTEA.2009.5227871

    2010-01-01

    Irreversible logic circuits dissipate heat for every bit of information that is lost. Information is lost when the input vector cannot be recovered from its corresponding output vector. Reversible logic circuit naturally takes care of heating because it implements only the functions that have one-to-one mapping between its input and output vectors. Therefore reversible logic design becomes one of the promising research directions in low power dissipating circuit design in the past few years and has found its application in low power CMOS design, digital signal processing and nanotechnology. This paper presents the efficient approaches for designing reversible fast adders that implement carry look-ahead and carry-skip logic. The proposed 16-bit high speed reversible adder will include IG gates for the realization of its basic building block. The IG gate is universal in the sense that it can be used to synthesize any arbitrary Boolean-functions. The IG gate is parity preserving, that is, the parity of the input...

  11. Protective Effect of Zizphus Vulgaris Extract, on Liver Toxicity in Laboratory Rats

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    S Ebrahimi

    2011-06-01

    Full Text Available Introduction & Objective: Some of natural and synthetic products have antioxidant properties which protect the liver against the destructive factors. This study aimed to investigate the effect of Zizphus Vulgaris extracts on mice liver. Materials & Methods: This experimental study was conducted at Yasouj University of Medical Sciences in 2010 on 30 healthy adult male Wistar rats. Animals were randomly divided into five equal groups: the control group (receiving, olive oil, control group (receiving olive oil and carbon tetrachloride and three intervention groups (receiving different dose of carbon tetrachloride and olive oil groups. The intervention group was given daily doses of 200, 400 and 600 mg per Kg of Zizphus Vulgaris extract by gavage respectively. After 45 days, the amount of liver enzymes, total protein, albumin and bilirubin in animal’s sera were measured. Data were analyzed by the SPSS software, using ANOVA and t-test. Results: The concentration of total protein, albumin, AST, ALT, ALP in test groups I, II and III receiving Z.Vulgaris extract (200, 400 and 600 mg/kg weight compared with control group were statistically not significant. Consumption of Z.Vulgaris reduced the bilirubin concentration in test groups I and II but this decrease was significant only in the test group I Increasing of Z.Vulgaris dose in the test group III (600 mg Z.Vulgaris per kg body weight showed increase in the level of serum bilirubin. Increase in the ratio of liver weight to body weight of rats in groups I and III in comparison with control groups was noticed although this difference was not statistically significant. Findings of this study revealed that dosage of 600 mg/kg extract of Z.Vulgaris caused significant improvements in CCl4 induced liver necrosis (P <0.01 and reduced portal cells inflammation (P <0.01. Dose of 400 mg/kg of Z.Vulgaris induced some destruction and necrosis of liver cells in animals but significant reduction of portal cells

  12. Orally administered extract from Prunella vulgaris attenuates spontaneous colitis in mdr1a-/-mice

    Institute of Scientific and Technical Information of China (English)

    Kelley; MK; Haarberg; Meghan; J; Wymore; Brand; Anne-Marie; C; Overstreet; Catherine; C; Hauck; Patricia; A; Murphy; Jesse; M; Hostetter; Amanda; E; Ramer-Tait; Michael; J; Wannemuehler

    2015-01-01

    AIM: To investigate the ability of a Prunella vulgaris(P. vulgaris) ethanolic extract to attenuate spontaneous typhlocolitis in mdr1a-/- mice. METHODS: Vehicle(5% ethanol) or P. vulgaris ethanolic extract(2.4 mg/d) were administered daily by oral gavage to mdr1a-/- or wild type FVBWT mice from 6 wk of age up to 20 wk of age. Clinical signs of disease were noted by monitoring weight loss. Mice experiencingweight loss in excess of 15% were removed from the study. At the time mice were removed from the study, blood and colon tissue were collected for analyses that included histological evaluation of lesions, inflammatory cytokine levels, and myeloperoxidase activity. RESULTS: Administration of P. vulgaris extracts to mdr1a-/- mice delayed onset of colitis and reduced severity of mucosal inflammation when compared to vehicle-treated mdr1a-/- mice. Oral administration of the P. vulgaris extract resulted in reduced(P < 0.05) serum levels of IL-10(4.6 ± 2 vs 19.4 ± 4), CXCL9(1319.0 ± 277 vs 3901.0 ± 858), and TNFα(9.9 ± 3 vs 14.8 ± 1) as well as reduced gene expression by more than two-fold for Ccl2, Ccl20, Cxcl1, Cxcl9, IL-1 α, Mmp10, VCAM-1, ICAM, IL-2, and TNFα in the colonic mucosa of mdr1a-/- mice compared to vehicle-treated mdr1a-/-mice. Histologically, several microscopic parameters were reduced(P < 0.05) in P. vulgaris-treated mdr1a-/-mice, as was myeloperoxidase activity in the colon(2.49 ± 0.16 vs 3.36 ± 0.06, P < 0.05). The numbers of CD4+ T cells(2031.9 ± 412.1 vs 5054.5 ± 809.5) and germinal center B cells(2749.6 ± 473.7 vs 4934.0 ± 645.9) observed in the cecal tonsils of P. vulgaris-treated mdr1a-/- were significantly reduced(P < 0.05) from vehicle-treated mdr1a-/- mice. Vehicle-treated mdr1a-/- mice were found to produce serum antibodies to antigens derived from members of the intestinal microbiota, indicative of severe colitis and a loss of adaptive tolerance to the members of the microbiota. These serum antibodies were greatly reduced or

  13. Effects of magnetic field on the antioxidant defense system of recirculation-cultured Chlorella vulgaris.

    Science.gov (United States)

    Wang, Hai-Ying; Zeng, Xiao-Bo; Guo, Si-Yuan; Li, Zong-Tao

    2008-01-01

    Little is known about the influence of magnetic fields (MF) on growth of microalgae such as Chlorella vulgaris, which has been consumed as health food for various nutritional and pharmacological effects. This preliminary study investigated whether static MF can modulate the antioxidant system in C. vulgaris by exposing the cells to static MF generated by dual yoke electromagnets with magnetic flux density of 10-50 mT for 12 h. After exposure to 10-35 mT for 12 h, the activity of superoxide dismutases and peroxidase increased significantly compared to control cells. However, a remarkable increase of catalase activity occurred at 45 and 50 mT. The lipid peroxidation of algae cells determined by production of thiobarbituric acid-reactive substances was much increased when exposed to 35, 45, and 50 mT of MF. The scavenging ability of 2,2-diphenyl-1-picrylhydrazyl radical was decreased markedly while there was no variation of total carotenoids content in C. vulgaris cells. Assay of specific growth rate in 72 h cultivation after MF exposure was also conducted. In groups after exposure to 10-35 mT of MF, specific growth rate was significantly increased. These results suggest that 10-35 mT of static MF exposure could promote the growth of C. vulgaris and regulate its antioxidant defense system to protect cells efficiently, which could possibly enhance the growth of C. vulgaris in industrialized cultivation by MF.

  14. Magnesium Uptake by the Green Microalga Chlorella vulgaris in Batch Cultures.

    Science.gov (United States)

    Ben Amor-Ben Ayed, Hela; Taidi, Behnam; Ayadi, Habib; Pareau, Dominique; Stambouli, Moncef

    2016-03-01

    The accumulation (internal and superficial distribution) of magnesium ions (Mg(2+)) by the green freshwater microalga Chlorella vulgaris (C. vulgaris) was investigated under autotrophic culture in a stirred photobioreactor. The concentrations of the three forms of Mg(2+) (dissolved, extracellular, and intracellular) were determined with atomic absorption spectroscopy during the course of C. vulgaris growth. The proportions of adsorbed (extracellular) and absorbed (intracellular) Mg(2+) were quantified. The concentration of the most important pigment in algal cells, chlorophyll a, increased over time in proportion to the increase in the biomass concentration, indicating a constant chlorophyll/biomass ratio during the linear growth phase. The mean-average rate of Mg(2+) uptake by C. vulgaris grown in a culture medium starting with 16 mg/l of Mg(2+) concentration was measured. A clear relationship between the biomass concentration and the proportion of the Mg(2+) removal from the medium was observed. Of the total Mg(2+) present in the culture medium, 18% was adsorbed on the cell wall and 51% was absorbed by the biomass by the end of the experiment (765 h). Overall, 69% of the initial Mg(2+) were found to be removed from the medium. This study supported the kinetic model based on a reversible first-order reaction for Mg(2+) bioaccumulation in C. vulgaris, which was consistent with the experimental data.

  15. Mechanisms of Eu(III) and Cm(III) Association With Chlorella Vulgaris

    Science.gov (United States)

    Ozaki, T.; Kimura, T.; Ohnuki, T.; Francis, A. J.

    2002-12-01

    Association of Eu(III) and Cm(III) with Chlorella vulgaris and cellulose was studied by a batch method, time-resolved laser-induced fluorescence spectroscopy (TRLFS) and extended X-ray absorption fine structure (EXAFS). The kinetics study performed by a batch method showed that the maximum adsorption of Eu(III) and Cm(III) on C. vulgaris was attained within three minutes of contact time, and afterwards the percentage adsorption decreased with time due to exudates released from C. vulgaris with affinity for Eu(III) and Cm(III). TRLFS showed that the short-term adsorption of Eu(III) on C. vulgaris was attributed to their coordination with the cell wall components comprised of cellulose. TRLFS also demonstrated that Eu(III) coordinated with the functional groups of cellulose very weakly in spite of the large distribution coefficients observed. EXAFS analysis showed the local structure around the Eu(III) adsorbed on cellulose and with C. vulgaris was similar. These results indicate that the reactions both at cell surfaces through the adsorption as well as in solution phases through chelation with the exudates are important in estimating the environmental behavior of Eu(III) and Cm(III) in aqueous environments.

  16. Development of novel microsatellite markers for strain-specific identification of Chlorella vulgaris.

    Science.gov (United States)

    Jo, Beom-Ho; Lee, Chang Soo; Song, Hae-Ryong; Lee, Hyung-Gwan; Oh, Hee-Mock

    2014-09-01

    A strain-specific identification method is required to secure Chlorella strains with useful genetic traits, such as a fast growth rate or high lipid productivity, for application in biofuels, functional foods, and pharmaceuticals. Microsatellite markers based on simple sequence repeats can be a useful tool for this purpose. Therefore, this study developed five novel microsatellite markers (mChl-001, mChl-002, mChl-005, mChl-011, and mChl-012) using specific loci along the chloroplast genome of Chlorella vulgaris. The microsatellite markers were characterized based on their allelic diversities among nine strains of C. vulgaris with the same 18S rRNA sequence similarity. Each microsatellite marker exhibited 2~5 polymorphic allele types, and their combinations allowed discrimination between seven of the C. vulgaris strains. The two remaining strains were distinguished using one specific interspace region between the mChl-001 and mChl-005 loci, which was composed of about 27 single nucleotide polymorphisms, 13~15 specific sequence sites, and (T)n repeat sites. Thus, the polymorphic combination of the five microsatellite markers and one specific locus facilitated a clear distinction of C. vulgaris at the strain level, suggesting that the proposed microsatellite marker system can be useful for the accurate identification and classification of C. vulgaris.

  17. Quality of life in acne vulgaris: Relationship to clinical severity and demographic data

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    Aayush Gupta

    2016-01-01

    Full Text Available Background: Acne vulgaris is known to impair many aspects of quality of life. However, the correlation of this impairment with clinical severity remains equivocal despite various school, community and hospital-based studies. Aim: A hospital-based study was undertaken to measure the impairment of quality of life of patients of acne vulgaris and correlate it with the severity of lesions. Methods: This was a cross-sectional, questionnaire-based study in a cohort of 100 patients of acne vulgaris attending the outpatient department of our referral hospital. A physician measured the severity of lesions using the global acne grading system, and patients assessed quality of life by completing a questionnaire (Cardiff acne disability index. A correlation of these two was done; some additional correlations were brought out through demographic data collected from the patients. Results: There was no correlation between the severity of acne vulgaris and an impaired quality of life. Patients who consumed alcohol and/or smoked cigarettes were found to have an impaired quality of life. While the severity of acne progressively lessened in older patients, the impact on quality of life increased. Limitations: The sample size was small and there was a lack of guaranteed reliability on the self-reported quality of life. Conclusion: The severity of acne vulgaris does not correlate with impairment in quality of life.

  18. The use of sodium sulfacetamide 10%-sulfur 5% emollient foam in the treatment of acne vulgaris.

    Science.gov (United States)

    Del Rosso, James Q

    2009-08-01

    Acne vulgaris is the most common disorder encountered in ambulatory clinical practice comprising 11.3 percent of office visits to dermatologists in 2005.(1) By comparison, eczematous dermatoses, psoriasis, and skin cancer accounted for 6.2, 3.5, and 10 percent of office visits, respectively.(1) A variety of topical therapeutic options are available for treatment of acne vulgaris, including benzoyl peroxide, antibiotics, retinoids, azelaic acid, and sodium sulfacetamide-sulfur.(2,3) Sodium sulfacetamide 10%-sulfur 5% has been used for the topical treatment of seborrheic dermatitis, acne vulgaris, and rosacea since the mid-1950s and is available in a variety of formulations, including lotions, creams, cleansers, and emollient foams.(4) Recently, an emollient foam sodium sulfacetamide 10%-sulfur 5% formulation indicated for topical therapy of acne vulgaris, rosacea, and seborrheic dermatitis has become available.(5) This article provides an overview of the sodium sulfacetamide 10%-sulfur 5% emollient foam and reports the results of a case report series of patients with acne vulgaris treated with sodium sulfacetamide 10%-sulfur 5% emollient foam as monotherapy or in combination with other topical acne products.

  19. The Optimizing of Growth and Quality of Chlorella vulgaris as ASUH feed supplement for Broiler

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    Salvia Salvia

    2014-01-01

    Full Text Available Chlorella is spherical single celled freshwater micro-algae.   Chlorella  has potentials as element of ration or natural ASUH (safe, healthy, whole and halal feed supplement for it contains nutrition and active component, decreases cholesterol level and resulting darker yolk.  Chlorella vulgaris  is type of green algae   which, its economical potential need to be revealed. Variety of components of growing media is one of factors determining quality of microalgae. In terms of mass production, it is important to find correct, cheap and easy to feed nutrition for breeders. The objective of the research is to find out the optimizing of growth and quality of Chlorella vulgaris   as ASUH feed supplement for broiler. Test using sedgwick rafter method conducted to find out the optimizing of growth and quality of  Chlorella vulgaris  while AOAC method applied to test quality of its nutrition. The result shows that.  Chlorella vulgaris  grew well at technical medium 10 % of Phyto-s, crude protein 57.63%, fat  5.84%, b Carotene 6.44 mg/gram, Vitamin C 4.12 mg/gram and vitamin E 1.32 mg/gram. We can say that Chlorella vulgaris  potential to be natural and ASUH feed supplement and Phyto-s can be used as nutrition for mass production.

  20. Efficacy of Essential Oils of Thymus vulgaris and Origanum vulgare on Echinococcus granulosus

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    P. E. Pensel

    2014-01-01

    Full Text Available The aim of the present work was to determine the in vitro effect of T. vulgaris and O. vulgare essential oils against E. granulosus protoscoleces and cysts. Essential oils were added to the medium resulting in thymol final concentrations of 10 μg/mL. The essential oils had a time-dependent effect provoking the complete loss of protoscolex viability after 72 days of postincubation. The results were confirmed at the ultrastructure level. Loss of infectivity in protoscoleces incubated with O. vulgare after 60 days was observed. On the other hand, the weight of cysts recorded in mice inoculated with T. vulgaris treated protoscoleces was significantly lower than that obtained in control group. Gamma-glutamyl-transpeptidase activity was readily detected in the culture supernatant of protoscoleces treated either with the essential oils or thymol. T. vulgaris and O. vulgare essential oils and thymol can induce cell apoptosis of protoscoleces after short incubation times. The efficacy of T. vulgaris and O. vulgare essential oils was also demonstrated in vitro on E. granulosus murine cysts. Our data suggest that essential oils of T. vulgaris and O. vulgare have anthelmintic effect against protoscoleces and cysts of E. granulosus.

  1. Decreased profilaggrin expression in ichthyosis vulgaris is a result of selectively impaired posttranscriptional control.

    Science.gov (United States)

    Nirunsuksiri, W; Presland, R B; Brumbaugh, S G; Dale, B A; Fleckman, P

    1995-01-13

    Ichthyosis vulgaris is an autosomal dominant disorder of keratinization characterized by mild hyperkeratosis and reduced or absent keratohyalin granules in the epidermis. Profilaggrin, a major component of keratohyalin granules, is reduced or absent from the skin of individuals with ichthyosis vulgaris. In this report, we have further characterized the molecular basis of low profilaggrin expression, which occurs in this disease. In situ hybridization revealed little profilaggrin mRNA in ichthyosis vulgaris-affected epidermis. In keratinocytes cultured from the epidermis of affected individuals, the abundance of profilaggrin was reduced to less than 10% of normal controls, while the mRNA level was decreased to 30-60% of controls. Expression of K1 and loricrin, other markers of epidermal differentiation, were not affected. Nuclear run-on assays indicated that the decrease in mRNA levels was not caused by aberrant transcription. Nucleotide sequencing of 5'-upstream, 3'-non-coding, and flanking regions of the profilaggrin gene from ichthyosis vulgaris-affected individuals revealed only minor changes, probably due to genetic polymorphisms. Our results indicate that defective profilaggrin expression in ichthyosis vulgaris is a result of selectively impaired posttranscriptional control.

  2. Efficacy of Essential Oils of Thymus vulgaris and Origanum vulgare on Echinococcus granulosus.

    Science.gov (United States)

    Pensel, P E; Maggiore, M A; Gende, L B; Eguaras, M J; Denegri, M G; Elissondo, M C

    2014-01-01

    The aim of the present work was to determine the in vitro effect of T. vulgaris and O. vulgare essential oils against E. granulosus protoscoleces and cysts. Essential oils were added to the medium resulting in thymol final concentrations of 10 μg/mL. The essential oils had a time-dependent effect provoking the complete loss of protoscolex viability after 72 days of postincubation. The results were confirmed at the ultrastructure level. Loss of infectivity in protoscoleces incubated with O. vulgare after 60 days was observed. On the other hand, the weight of cysts recorded in mice inoculated with T. vulgaris treated protoscoleces was significantly lower than that obtained in control group. Gamma-glutamyl-transpeptidase activity was readily detected in the culture supernatant of protoscoleces treated either with the essential oils or thymol. T. vulgaris and O. vulgare essential oils and thymol can induce cell apoptosis of protoscoleces after short incubation times. The efficacy of T. vulgaris and O. vulgare essential oils was also demonstrated in vitro on E. granulosus murine cysts. Our data suggest that essential oils of T. vulgaris and O. vulgare have anthelmintic effect against protoscoleces and cysts of E. granulosus.

  3. Global transcriptional, physiological and metabolite analyses of Desulfovibrio vulgaris Hildenborough responses to salt adaptation

    Energy Technology Data Exchange (ETDEWEB)

    He, Z.; Zhou, A.; Baidoo, E.; He, Q.; Joachimiak, M. P.; Benke, P.; Phan, R.; Mukhopadhyay, A.; Hemme, C.L.; Huang, K.; Alm, E.J.; Fields, M.W.; Wall, J.; Stahl, D.; Hazen, T.C.; Keasling, J.D.; Arkin, A.P.; Zhou, J.

    2009-12-01

    The response of Desulfovibrio vulgaris Hildenborough to salt adaptation (long-term NaCl exposure) was examined by physiological, global transcriptional, and metabolite analyses. The growth of D. vulgaris was inhibited by high levels of NaCl, and the growth inhibition could be relieved by the addition of exogenous amino acids (e.g., glutamate, alanine, tryptophan) or yeast extract. Salt adaptation induced the expression of genes involved in amino acid biosynthesis and transport, electron transfer, hydrogen oxidation, and general stress responses (e.g., heat shock proteins, phage shock proteins, and oxidative stress response proteins). Genes involved in carbon metabolism, cell motility, and phage structures were repressed. Comparison of transcriptomic profiles of D. vulgaris responses to salt adaptation with those of salt shock (short-term NaCl exposure) showed some similarity as well as a significant difference. Metabolite assays showed that glutamate and alanine were accumulated under salt adaptation, suggesting that they may be used as osmoprotectants in D. vulgaris. A conceptual model is proposed to link the observed results to currently available knowledge for further understanding the mechanisms of D. vulgaris adaptation to elevated NaCl.

  4. In vitro activity of heather [Calluna vulgaris (L.) Hull] extracts on selected urinary tract pathogens

    Science.gov (United States)

    Vučić, Dragana M.; Petković, Miroslav R.; Rodić-Grabovac, Branka B.; Stefanović, Olgica D.; Vasić, Sava M.; Čomić, Ljiljana R.

    2014-01-01

    Calluna vulgaris L. Hull (Ericaceae) has been used for treatment of urinary tract infections in traditional medicine. In this study we analyzed in vitro antibacterial activity of the plant extracts on different strains of Escherichia coli, Enterococcus faecalis and Proteus vulgaris, as well as the concentrations of total phenols and flavonoids in the extracts. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined. The concentrations of total phenols were examined by using Folin-Ciocalteu reagent and ranged between 67.55 to 142.46 mg GAE/g. The concentrations of flavonoids in extracts were determined using spectrophotometric method with aluminum chloride and the values ranged from 42.11 to 63.68 mg RUE/g. The aqueous extract of C. vulgaris showed a significant antibacterial activity. The values of MIC were in the range from 2.5 mg/ml to 20 mg/ml for this extract. Proteus vulgaris strains were found to be the most sensitive. The results obtained suggest that all tested extracts of C. vulgaris inhibit the growth of human pathogens, especially the aqueous extract. PMID:25428676

  5. Polymorphism of the catechol-O-methyltransferase gene in Han Chinese patients with psoriasis vulgaris

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    Lin Gao

    2009-01-01

    Full Text Available Psoriasis vulgaris is defined by a series of linked cellular changes in the skin: hyperplasia of epidermal keratinocytes, vascular hyperplasia and ectasia, and infiltration of T lymphocytes, neutrophils and other types of leukocytes in the affected skin. Catechol-O-methyltransferase ( COMT 158 polymorphism can reduce the activity of the COMT enzyme that may trigger defective differentiation of keratinocytes in psoriasis. Immunocytes can degrade and inactivate catecholamines via monamine oxidase (MAO and COMT in the cells. We hypothesized that the COMT-158 G > A polymorphism was associated with the risk of psoriasis vulgaris in Han Chinese people. In a hospital-based case-control study, 524 patients with psoriasis vulgaris and 549 psoriasis-free controls were studied. COMT-158 G > A polymorphism was genotyped using the PCR sequence-specific primer (PCR-SSP technique. We found no statistically significant association between the COMT-158 allele A and the risk of psoriasis vulgaris (p = 0.739 adjusted OR = 1.03; 95% CI = 0.81-1.31. This suggests that the COMT-158 G > A polymorphism may not contribute to the etiology of psoriasis vulgaris in the Han Chinese population.

  6. Efficacy of Essential Oils of Thymus vulgaris and Origanum vulgare on Echinococcus granulosus

    Science.gov (United States)

    Pensel, P. E.; Maggiore, M. A.; Gende, L. B.; Eguaras, M. J.; Denegri, M. G.; Elissondo, M. C.

    2014-01-01

    The aim of the present work was to determine the in vitro effect of T. vulgaris and O. vulgare essential oils against E. granulosus protoscoleces and cysts. Essential oils were added to the medium resulting in thymol final concentrations of 10 μg/mL. The essential oils had a time-dependent effect provoking the complete loss of protoscolex viability after 72 days of postincubation. The results were confirmed at the ultrastructure level. Loss of infectivity in protoscoleces incubated with O. vulgare after 60 days was observed. On the other hand, the weight of cysts recorded in mice inoculated with T. vulgaris treated protoscoleces was significantly lower than that obtained in control group. Gamma-glutamyl-transpeptidase activity was readily detected in the culture supernatant of protoscoleces treated either with the essential oils or thymol. T. vulgaris and O. vulgare essential oils and thymol can induce cell apoptosis of protoscoleces after short incubation times. The efficacy of T. vulgaris and O. vulgare essential oils was also demonstrated in vitro on E. granulosus murine cysts. Our data suggest that essential oils of T. vulgaris and O. vulgare have anthelmintic effect against protoscoleces and cysts of E. granulosus. PMID:25180033

  7. Epidemiology of pemphigus vulgaris in the Northeast China: a 10-year retrospective study.

    Science.gov (United States)

    Zhu, Xiaoling; Pan, Jisheng; Yu, Zhiming; Wang, Yanhua; Cai, Limin; Zheng, Shuyun

    2014-01-01

    The aim of the present study was to evaluate the incidence, epidemiological, clinical and therapeutic features of patients with pemphigus vulgaris in China. We retrospectively investigated a total of 221 patients with pemphigus vulgaris. The parameters including age at diagnosis/onset, sex, diagnostic methods, hospitalizations, side-effects with different treatments, pemphigus vulgaris-associated diseases, the therapeutic features and duration of follow up were evaluated. The male : female ratio was 1:1.40. The incidence rate was obviously higher in two age groups, 31-40 years and 41-50 years. The mean age at onset was 44.19 ± 21.45 years. The majority of patients (56.56%) presented the mucocutaneous type. In addition, we found that various doses of corticosteroids (including the mean initial dose, maximum control dose, total dose before reducing and hospitalized total dose) exhibited statistical differences between only corticosteroids and corticosteroids-immunosuppressant groups in mild, moderate and severe pemphigus vulgaris (P pemphigus vulgaris most frequently occurred in the 41-50-year age group in China and the majority of patients manifested mucocutaneous lesions. Importantly, the incidence rate in females was higher than in males. The patients who needed combined immunosuppressant treatment, usually needed higher doses of corticosteroids initially, to control the disease and over the total course of treatment.

  8. The use of biochip immunofluorescence microscopy for the diagnosis of Pemphigus vulgaris.

    Science.gov (United States)

    Russo, Irene; Saponeri, Andrea; Peserico, Andrea; Alaibac, Mauro

    2014-06-01

    Pemphigus vulgaris is an autoimmune intraepithelial blistering skin disease characterized by the presence of circulating autoantibodies directed against surfaces of keratinocytes. Diagnosis is generally based on clinical features, histology, direct and indirect immunofluorescence and ELISA. This study describes a new BIOCHIP mosaic-based indirect immunofluorescence technique based on recombinant antigenic substrates and transfected cells. We investigated the diagnostic use of BIOCHIP for the serological diagnosis of Pemphigus vulgaris. Autoantibodies against desmoglein 3 were detected in 97.62% of patients (41/42) with P. vulgaris. There were no positive results in the negative control group. Our study revealed that BIOCHIP has high sensitivity and specificity comparable to that of the ELISA assays. Therefore the BIOCHIP technique seems to be an appropriate method for the diagnosis of P. vulgaris as it has been shown to be a simple, standardized and readily available novel tool, which could facilitate the diagnosis of this autoimmune bullous disease. We suggest that it could be used as an initial screening test to identify patients with P. vulgaris before using the ELISA approach.

  9. Association of HSD17B3 and HSD3B1 polymorphisms with acne vulgaris in Southwestern Han Chinese.

    Science.gov (United States)

    Yang, Xiao-Yan; Wu, Wen-Juan; Yang, Cheng; Yang, Ting; He, Jun-Dong; Yang, Zhi; He, Li

    2013-01-01

    Acne vulgaris is a very common skin disorder. Previous studies have indicated that genetic background factors play key roles in the onset of acne. Our previous investigation implicated several genes in the androgen metabolism pathway with acne vulgaris in the Han Chinese population. Thus, we further investigated genes and genetic variants that play important roles in this pathway for their relationship with the pathology of acne. In this study, a total of 610 subjects, including 403 acne patients and 207 healthy controls, were genotyped for 15 single-nucleotide polymorphisms in HSD3B1 and HSD17B3 genes. This study shows that rs6428829 in HSD3B1 was associated with acne vulgaris in Han patients from Southwest China, even after adjusting for age and sex. The GG genotype was associated with an increased risk of acne vulgaris (p acne vulgaris (p acne vulgaris in the case-control study (p acne vulgaris. Based on these analyses, our study indicates that the cutaneous androgen metabolism-regulated genes HSD3B1 and HSD17B3 increase the susceptibility to acne vulgaris in Han Chinese from Southwest China.

  10. A study on aromatic profiles of Thymus hyemalis and Spanish T. vulgaris essential oils at five physiological stages

    Science.gov (United States)

    Shrubs of clonal selections of Thymus hyemalis L. and Spanish T. vulgaris subsp. vulgaris were harvested at five phenological stages during the plant growing cycle: vegetative (VEG), floral (FL), floral-fructification (FL-FR), fructification (FR), and passed fructification (FR-pas). The volatile pro...

  11. The inhibitory effect of Thymus vulgaris extracts on the planktonic form and biofilm structures of six human pathogenic bacteria

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    Zinab Mohsenipour

    2015-06-01

    Full Text Available Objective: Microorganisms are responsible for many problems in industry and medicine because of biofilm formation. Therefore, this study was aimed to examine the effect of Thymus vulgaris (T. vulgaris extracts on the planktonic form and biofilm structures of six pathogenic bacteria. Materials and methods: Antimicrobial activities of the plant extracts against the planktonic form of the bacteria were determined using the disc diffusion method. MIC and MBC values were evaluated using macrobroth dilution technique. Anti-biofilm effects were assessed by microtiter plate method. Results: According to disc diffusion test (MIC and MBC, the ability of Thymus vulgaris (T. vulgaris extracts for inhibition of bacteria in planktonic form was confirmed. In dealing with biofilm structures, the inhibitory effect of the extracts was directly correlated to their concentration. Except for the inhibition of biofilm formation, efficacy of each extract was independent from type of solvent. Conclusion: According to the potential of Thymus vulgaris (T. vulgaris extracts to inhibit the test bacteria in planktonic and biofilm form, it can be suggested that Thymus vulgaris(T. vulgaris extracts can be applied as antimicrobial agents against the pathogenic bacteria particularly in biofilm forms.

  12. Gas chromatography-mass spectrometry analysis of different organic crude extracts from the local medicinal plant of Thymus vulgaris L

    Institute of Scientific and Technical Information of China (English)

    Laila Salim Al Hashmi; Mohammad Amzad Hossain; Afaf Mohammed Weli; Qasim Al-Riyami; Jamal Nasser Al-Sabahi

    2013-01-01

    Objective: To isolate and analyze the chemical composition in different crude extracts of from the leaves of locally grown of Thymus vulgaris L (T. vulgaris) by gas chromatography-mass spectrometry (GC-MS). Methods: The shade dried leaves powder was extracted with methanol by using Soxhlet extractor. Methanol crude extracts of T. vulgaris and the derived fractions of hexane, chloroform, ethyl acetate and butanol were obtained. Results: Qualitative analyses of various organic crude extracts of T. vulgaris by using GC-MS showed that there were different types of high and low molecular weight compounds. Most of the isolated and identified compounds by GC-MS in the crude extracts are basically biologically important. Further, the T. vulgaris leaf possessed certain characteristics that can be ascribed to cultivation on a domestic plantation. The crude extracts were prepared from the powder leaves of T. vulgaris for respective compounds can be chosen on the basis of above GC-MS analysis. Conclusions: All the major compounds were identified and characterized by spectroscopic method in different organic crude extracts of T. vulgaris are biologically active molecules. Thus the identification of a good number of compounds in various crude extracts of T. vulgaris might have some ecological role.

  13. Gas chromatography-mass spectrometry analysis of different organic crude extracts from the local medicinal plant of Thymus vulgaris L

    Institute of Scientific and Technical Information of China (English)

    Laila; Salim; Al; Hashmi; Mohammad; Amzad; Hossain; Afaf; Mohammed; Weli; Qasim; Al-Riyami; Jamal; Nasser; Al-Sabahi

    2013-01-01

    Objective:To isolate and analyze the chemical composition in different crude extracts of from the leaves of locally grown of Thymus vulgaris L(T.vulgaris)by gas chromatography-mass spectrometry(GC-MS).Methods:The shade dried leaves powder was extracted with methanol by using Soxhlet extractor.Methanol crude extracts of T.vulgaris and the derived fractions of hexane,chloroform,ethyl acetate and butanol were obtained.Results:Qualitative analyses of various organic crude extracts of T.vulgaris by using GC-MS showed that there were different types of high and low molecular weight compounds.Most of the isolated and identified compounds by GC-MS in the crude extracts are basically biologically important.Further,the T.vulgaris leaf possessed certain characteristics that can be ascribed to cultivation on a domestic plantation.The crude extracts were prepared from the powder leaves of T.vulgaris for respective compounds can be chosen on the basis of above GC-MS analysis.Conclusions:All the major compounds were identified and characterized by spectroscopic method in different organic crude extracts of T.vulgaris are biologically active molecules.Thus the identification of a good number of compounds in various crude extracts of T.vulgaris might have some ecological role.

  14. Gas chromatography–mass spectrometry analysis of different organic crude extracts from the local medicinal plant of Thymus vulgaris L

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    Laila Salim Al Hashmi

    2013-01-01

    Conclusions: All the major compounds were identified and characterized by spectroscopic method in different organic crude extracts of T. vulgaris are biologically active molecules. Thus the identification of a good number of compounds in various crude extracts of T. vulgaris might have some ecological role.

  15. Genetic differences between wild and hatchery populations of Diplodus sargus and D. vulgaris inferred from RAPD markers: implications for production and restocking programs design.

    Science.gov (United States)

    Pereira, J C; Lino, P G; Leitão, A; Joaquim, S; Chaves, R; Pousão-Ferreira, P; Guedes-Pinto, H; dos Santos, M Neves

    2010-01-01

    Restocking and stock enhancement programs are now recognized as an important tool for the management of fishery resources. It is important, however, to have an adequate knowledge on the genetic population structure of both the released stock and the wild population before carrying out such programs. In this study, random amplified polymorphic DNA (RAPD) markers were applied to assess genetic diversity and population structure of wild and hatchery populations of the white seabream Diplodus sargus and the common two-banded seabream D. vulgaris (Sparidae). The estimated values for intrapopulation genetic variation, measured using the percentage of polymorphic loci (%P), Shannon index (H'), and Nei's gene diversity (h), showed high values for all populations. The percentage of genetic variation within D. sargus and D. vulgaris populations, based on coefficient of gene differentiation, reached 82.5% and 90% of the total genetic variation, respectively. An undeniable decrease in genetic variation was found in both hatchery populations, particularly in D. sargus, compared to the wild ones. However, the high values of variation within all populations and the low levels of genetic variation among populations did not indicate inbreeding or depression effects, thus indicating a fairly proper hatchery management. Nevertheless, the results of this study highlight the importance of monitoring the genetic variation of hatchery populations, particularly those to be used in restocking programs. The creation of a genetic baseline database will contribute to a more efficient conservation management and to the design of genetically sustainable restocking programs.

  16. Effect of Nigella sativa alcoholic extract and oil, as well as Phaseolus vulgaris (kidney bean) lectin on the ultrastructure of Trichomonas vaginalis trophozoites.

    Science.gov (United States)

    Aminou, Heba AbdelKader; Alam-Eldin, Yosra Hussein; Hashem, Hanan Ahmed

    2016-09-01

    Trichomonas vaginalis is a parasitic protozoan that is the aetiological agent of trichomoniasis, the most common non-viral sexually transmitted disease worldwide. Currently, the compound of choice for the treatment of T. vaginalis infections is metronidazole, however, it has many side effects and an increase in metronidazole-resistant trichomoniasis has been observed. Medicinal plants could be a source of new antiprotozoal drugs with high activity, low toxicity and lower price. The present work was carried out to investigate the therapeutic potential of Nigella sativa alcoholic extract and oil, as well as Phaseolus vulgaris (kidney bean) lectin and their in vitro activity on the ultrastructure of T. vaginalis trophozoites in comparison to metronidazole, as detected by transmission electron microscope. Both N. sativa oil and P. vulgaris lectin showed high toxic effect as evidenced by severe cell damage with cytoplasmic and nuclear destruction, while the effect of N. sativa alcoholic extract was moderate. Therefore, these two extracts could offer an effective, cheaper and more safe alternative for metronidazole in treatment of trichomoniasis.

  17. Atividade antimicrobiana de Struthanthus vulgaris (erva-de-passarinho Antimicrobial activity of Struthantus vulgaris (erva-de-passarinho

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    O.M.C. Vieira

    2005-06-01

    Full Text Available As plantas do gênero Struthanthus são conhecidas como ervas-de-passarinho e parasitam pomares no Brasil, principalmente os de laranjeiras e goiabeiras. Na medicina popular são usadas nas afecções das vias respiratórias. O extrato hidroetanólico a 70% de folhas frescas de Struthanthus vulgaris apresentou atividade antimicrobiana contra amostras bacterianas Gram positiva e Gram negativa. Este extrato não apresentou, nas condições testadas, atividade contra fungos. As amostras bacterianas mais sensíveis ao extrato foram Bacillus cereus (ATCC 11778, Micrococcus luteus (ATCC 9341, Staphylococcus aureus (ATCC 6538, S. epidermidis (ATCC 12228 e P. aeruginosa (ATCC 27853, usando o método de difusão em agar. As frações obtidas, pela partição líquido-líquido do extrato hidroetanólico a 70%, com solventes de polaridades crescentes (clorofórmio, acetato de etila, n-butanol e água, apresentaram diferentes atividades inibitórias. A fração que apresentou a maior atividade contra bactéria Gram positiva (B. cereus e Gram negativa (P. aeruginosa foi aquela obtida com n-butanol. Nessa fração foram detectados flavonóides, taninos condensados (proantocianidinas e saponinas.Struthantus vulgaris (mistletoe is one of the most common hemiparasite species in Brazil. It occurs as a parasite of orchards, mainly in orange and guava trees. Some authors mention Struthantus use in traditional medicine for respiratory diseases treatment. Fresh leaves concentrated hydroalcoholic extract showed antimicrobial activity against Gram positive and Gram negative bacterial samples. In tested conditions, these extracts did not show activity against fungi. The more susceptible bacterial samples to fresh leaves hydroalcoholic extract were Bacillus cereus (ATCC 11778, Micrococcus luteus (ATCC 9341, Staphylococcus aureus (ATCC 6538, S. epidermidis (ATCC 12228 and Pseudomonas aeruginosa (ATCC 27853. The method used for assessment of antimicrobial activity was agar

  18. Use of diluted urine for cultivation of Chlorella vulgaris.

    Science.gov (United States)

    Jaatinen, Sanna; Lakaniemi, Aino-Maija; Rintala, Jukka

    2016-01-01

    Our aim was to study the biomass growth of microalga Chlorella vulgaris using diluted human urine as a sole nutrient source. Batch cultivations (21 days) were conducted in five different urine dilutions (1:25-1:300), in 1:100-diluted urine as such and with added trace elements, and as a reference, in artificial growth medium. The highest biomass density was obtained in 1:100-diluted urine with and without additional trace elements (0.73 and 0.60 g L(-1), respectively). Similar biomass growth trends and densities were obtained with 1:25- and 1:300-diluted urine (0.52 vs. 0.48 gVSS L(-1)) indicating that urine at dilution 1:25 can be used to cultivate microalgal based biomass. Interestingly, even 1:300-diluted urine contained sufficiently nutrients and trace elements to support biomass growth. Biomass production was similar despite pH-variation from < 5 to 9 in different incubations indicating robustness of the biomass growth. Ammonium formation did not inhibit overall biomass growth. At the beginning of cultivation, the majority of the biomass consisted of living algal cells, while towards the end, their share decreased and the estimated share of bacteria and cell debris increased.

  19. Indocyanine green-laser thermolysis of acne vulgaris

    Science.gov (United States)

    Genina, Elina A.; Bashkatov, Alexey N.; Simonenko, Georgy V.; Tuchin, Valery V.; Yaroslavsky, Ilya V.; Altshuler, Gregory B.

    2005-08-01

    The near-infrared (NIR) laser radiation due to its high penetration depth is widely used in phototherapy and photothermolysis. In application to skin appendages a high selectivity of laser treatment is needed to prevent light action on surrounding tissues. Indocyanine Green (ICG) dye may provide a high selectivity of treatment due to effective ICG uploading by a target and its narrow band of considerable absorption just at the wavelength of the NIR diode laser. The goal of this study is to demonstrate the efficacy of the NIR diode laser photothermolysis in combination with topical application of ICG suggested for treatment of acne vulgaris. Two volunteers with back-located acne were enrolled. Skin sites of subjects were stained by ICG and irradiated by NIR laser-diode light (803 or 809 nm). The individual acne lesions were photothermally treated at 18 W/cm2 (803 nm, 0.5 sec) without skin surface cooling or at 200 W/cm2 (809 nm, 0.5 sec) with cooling. The results of the observations during a month after the treatment have shown that ICG stained acne inflammatory elements were destructed for light exposures of 0.5 sec.

  20. Structure and mechanical properties of Octopus vulgaris suckers.

    Science.gov (United States)

    Tramacere, Francesca; Kovalev, Alexander; Kleinteich, Thomas; Gorb, Stanislav N; Mazzolai, Barbara

    2014-02-06

    In this study, we investigate the morphology and mechanical features of Octopus vulgaris suckers, which may serve as a model for the creation of a new generation of attachment devices. Octopus suckers attach to a wide range of substrates in wet conditions, including rough surfaces. This amazing feature is made possible by the sucker's tissues, which are pliable to the substrate profile. Previous studies have described a peculiar internal structure that plays a fundamental role in the attachment and detachment processes of the sucker. In this work, we present a mechanical characterization of the tissues involved in the attachment process, which was performed using microindentation tests. We evaluated the elasticity modulus and viscoelastic parameters of the natural tissues (E ∼ 10 kPa) and measured the mechanical properties of some artificial materials that have previously been used in soft robotics. Such a comparison of biological prototypes and artificial material that mimics octopus-sucker tissue is crucial for the design of innovative artificial suction cups for use in wet environments. We conclude that the properties of the common elastomers that are generally used in soft robotics are quite dissimilar to the properties of biological suckers.

  1. Activity of Thymus vulgaris essential oil against Anisakis larvae.

    Science.gov (United States)

    Giarratana, F; Muscolino, D; Beninati, C; Giuffrida, A; Panebianco, A

    2014-07-01

    Anisakiasis is an important food-borne disease especially in countries with high fish consumption. The increase of cases of human disease and the virtual absence of effective treatments have prompted the research on new active compounds against Anisakis larvae. As well known, the disease is related to the consumption of raw or almost raw seafood products, but also marinated and/or salted fishery products, if the processing is insufficient to destroy nematode larvae can represent a risks for the consumers. In the light of the biocidal efficacy against different pathogens demonstrated for various essential oils, the aim of this work is to evaluate the effect of Thymus vulgaris essential oil (TEO) against anisakidae larvae. The TEO at 10% and 5% concentration in oil sunflower seeds, caused in vitro the death of all larvae within 14 h, with cuticle and intestinal wall damages. The results obtained showing a significant activity against Anisakis larvae, suggest further investigation on TEO as a larvicidal agent and on its potential use in the industrial marinating process. Copyright © 2014 Elsevier Inc. All rights reserved.

  2. Antinociceptive effects of hydroalcoholic extract of Thymus vulgaris.

    Science.gov (United States)

    Taherian, Abbas Ali; Babaei, Mahdi; Vafaei, Abbas Ali; Jarrahi, Morteza; Jadidi, Majid; Sadeghi, Hassan

    2009-01-01

    Previous investigation has shown that Thymus Vulgaris (TV) modulates pain. The aim of this work was to examine the role of TV on acute and chronic pain and compares its effect with dexamethasone (DEX) and stress (ST) by using hot plate, tail flick and formalin tests in mice. In this study male albino mice (25-30 g.) in 21 groups (n=147) were used. TV (100, 500 and 1000 mg/kg), DEX (0.5, 1 and 2 mg/kg) and vehicle (VEH) were injected 30 minutes before pain assessment tests. Stress was applied by 1 min swimming in cold water (18-22 degrees ). Acute and chronic pain was assessed by hot plate, tail flick and formalin tests. For assessment of the role of opioid receptors in antinoceception of TV extract, Naloxon (NAL, 2mg/kg, ip) as opioid receptor antagonist was injected before the injection of the more effective dose (500 mg/kg) of TV extract. Results indicated that TV, DEX and ST have analgesic effects in all tests (P<0.01 in comparison with control group). Above findings showed that TV extract, DEX and ST have modulatory effects on acute and chronic pain. Further research is required to determine the mechanisms by which TV extract has an inhibitory effect on pain sensation.

  3. Evaluation of solar dried thyme (Thymus vulgaris Linne) herbs

    Energy Technology Data Exchange (ETDEWEB)

    Balladin, Derrick A.; Headley, Oliver [University of the West Indies, Center for Resource Management and Environmental Studies, St Michael (Barbados)

    1999-07-01

    Thyme (Thymus vulgaris Linne) herbs can be dried at about 50degC reached an equilibrium moisture content after 12 h and 9.5 h using the wire basket solar dryer and oven drying method respectively. The initial moisture content (wet wt. basis), (final moisture content, dry wt. basis (dwb)) determined by the Dean-Stark toluene method, oven and microwave were 75.15% (10.0%), 75.12% (11.85%) and 72.31% (12.50%) respectively. Paired t-test ({alpha} = 0.05, 10 degrees of freedom) showed no significant difference between the Dean-Stark toluene and the oven methods, but a significant difference between these two methods and the microwaves method. The % essential oils extracted after drying by the oven and the wire basket solar methods were 0.5 and 0.6% (per 100 g dwb) respectively. The % oleoresin and ash content were 27% for both drying methods and 1.60, 2.03 and 2.25% for the fresh, oven dried and the wire basket solar dried herb respectively. (Author)

  4. Antioxidative effect of thyme (Thymus vulgaris in sunflower oil

    Directory of Open Access Journals (Sweden)

    Zofia Zaborowska

    2012-09-01

    Full Text Available   Background. Lipid oxidation is a main problem during food processing, storage and consumption leading to losses of quality, stability, safety and nutritive value. Antioxidants have been used to prevent oxidation changes and off – flavor development in food products. Aim of the research was to evaluate antioxidative effect of thyme ethanol extract on sunflower oil during its storage in different temperature conditions. Oil samples were stored in darkness at 4°C, 18°C, 38°C. Material and methods. Samples of thyme (thymus vulgaris were purchased at a local pharmacy in Poznań, Poland and sunflower oil was acquired from a local supermarket. Thyme extract was characterized by total polyphenol content. Antioxidant activity was estimated with use of DPPHand ABTSradicals scavenging methods. Ethanol extract of thyme at 1% level was added to sunflower oil. Peroxide value (PV, anisidine value (AV, totox value (TxV and fatty acids (FA content were taken as parameters for evaluation of effectiveness of thyme extract in stabilization of sunflower oil. Results. High polyphenol content, DPPHand ABTSradicals scavenging activity of ethanol thyme extract were evaluated. Results from different parameters were in agreement with other researchers, suggesting the antioxidant effect of thyme on antioxidant stability. Results show that thyme extract prolonged stability of sunflower oil and it may be a potent antioxidant for its stabilization. Conclusions. Ethanol thyme extract may be used as a natural antioxidant to prolong stability of oils.  

  5. Signaling dependent and independent mechanisms in pemphigus vulgaris blister formation.

    Science.gov (United States)

    Saito, Masataka; Stahley, Sara N; Caughman, Christopher Y; Mao, Xuming; Tucker, Dana K; Payne, Aimee S; Amagai, Masayuki; Kowalczyk, Andrew P

    2012-01-01

    Pemphigus vulgaris (PV) is an autoimmune epidermal blistering disease caused by autoantibodies directed against the desmosomal cadherin desmoglein-3 (Dsg3). Significant advances in our understanding of pemphigus pathomechanisms have been derived from the generation of pathogenic monoclonal Dsg3 antibodies. However, conflicting models for pemphigus pathogenicity have arisen from studies using either polyclonal PV patient IgG or monoclonal Dsg3 antibodies. In the present study, the pathogenic mechanisms of polyclonal PV IgG and monoclonal Dsg3 antibodies were directly compared. Polyclonal PV IgG cause extensive clustering and endocytosis of keratinocyte cell surface Dsg3, whereas pathogenic mouse monoclonal antibodies compromise cell-cell adhesion strength without causing these alterations in Dsg3 trafficking. Furthermore, tyrosine kinase or p38 MAPK inhibition prevents loss of keratinocyte adhesion in response to polyclonal PV IgG. In contrast, disruption of adhesion by pathogenic monoclonal antibodies is not prevented by these inhibitors either in vitro or in human skin explants. Our results reveal that the pathogenic activity of polyclonal PV IgG can be attributed to p38 MAPK-dependent clustering and endocytosis of Dsg3, whereas pathogenic monoclonal Dsg3 antibodies can function independently of this pathway. These findings have important implications for understanding pemphigus pathophysiology, and for the design of pemphigus model systems and therapeutic interventions.

  6. An antifungal defensin from Phaseolus vulgaris cv. 'Cloud Bean'.

    Science.gov (United States)

    Wu, Xiangli; Sun, Jian; Zhang, Guoqing; Wang, Hexiang; Ng, Tzi Bun

    2011-01-15

    An antifungal peptide with a defensin-like sequence and exhibiting a molecular mass of 7.3kDa was purified from dried seeds of Phaseolus vulgaris 'Cloud Bean'. The isolation procedure entailed anion exchange chromatography on DEAE-cellulose, affinity chromatography an Affi-gel blue gel, cation exchange chromatography on SP-Sepharose, and gel filtration by fast protein liquid chromatography on Superdex 75. Although the antifungal peptide was unadsorbed on DEAE-cellulose, it was adsorbed on both Affi-gel blue gel and SP-Sepharose. The antifungal peptide exerted antifungal activity against Mycosphaerella arachidicola with an IC(50) value of 1.8 μM. It was also active against Fusarium oxysporum with an IC(50) value of 2.2 μM. It had no inhibitory effect on HIV-1 reverse transcriptase when tested up to 100 μM. Proliferation of L1210 mouse leukemia cells and MBL2 lymphoma cells was inhibited by the antifungal peptide with an IC(50) of 10 μM and 40 μM, respectively.

  7. An antifungal peptide from Phaseolus vulgaris cv. brown kidney bean

    Institute of Scientific and Technical Information of China (English)

    Yau Sang Chan; Jack Ho Wong; Evandro Fei Fang; Wen Liang Pan; Tzi Bun Ng

    2012-01-01

    A 5.4-kDa antifungal peptide,with an N-terminal sequence highly homologous to defensins and inhibitory activity against Mycosphaerella arachidicola (IC5o=3 μM),Setospaeria turcica and Bipolaris maydis,was isolated from the seeds of Phaseolus vulgaris cv.brown kidney bean.The peptide was purified by employing a protocol that entailed adsorption on Affi-gel blue gel and Mono S and finally gel filtration on Superdex 75.The antifungal activity of the peptide against M.arachidicola was stable in the pH range 3-12 and in the temperature range 0℃ to 80℃.There was a slight reduction of the antifungal activity at pH 2 and 13,and the activity was indiscernible at pH 0,1,and 14.The activity at 90℃ and 100℃ was slightly diminished.Deposition of Congo red at the hyphal tips of M.arachidicola was induced by the peptide indicating inhibition of hyphal growth.The lack of antiproliferative activity of brown kidney bean antifungal peptide toward tumor cells,in contrast to the presence of such activity of other antifungal peptides,indicates that different domains are responsible for the antifungal and antiproliferative activities.

  8. An antifungal peptide from Phaseolus vulgaris cv. brown kidney bean.

    Science.gov (United States)

    Chan, Yau Sang; Wong, Jack Ho; Fang, Evandro Fei; Pan, Wen Liang; Ng, Tzi Bun

    2012-04-01

    A 5.4-kDa antifungal peptide, with an N-terminal sequence highly homologous to defensins and inhibitory activity against Mycosphaerella arachidicola (IC(50)= 3 μM), Setospaeria turcica and Bipolaris maydis, was isolated from the seeds of Phaseolus vulgaris cv. brown kidney bean. The peptide was purified by employing a protocol that entailed adsorption on Affi-gel blue gel and Mono S and finally gel filtration on Superdex 75. The antifungal activity of the peptide against M. arachidicola was stable in the pH range 3-12 and in the temperature range 0°C to 80°C. There was a slight reduction of the antifungal activity at pH 2 and 13, and the activity was indiscernible at pH 0, 1, and 14. The activity at 90°C and 100°C was slightly diminished. Deposition of Congo red at the hyphal tips of M. arachidicola was induced by the peptide indicating inhibition of hyphal growth. The lack of antiproliferative activity of brown kidney bean antifungal peptide toward tumor cells, in contrast to the presence of such activity of other antifungal peptides, indicates that different domains are responsible for the antifungal and antiproliferative activities.

  9. Redox Enzymes of Red Beetroot Vacuoles (Beta vulgaris L.

    Directory of Open Access Journals (Sweden)

    E.V. Pradedova

    2014-12-01

    Full Text Available Years of research have shown that some of the redox elements (enzymes, coenzymes, and co-substrate are isolated from each other kinetic and spatial manner (compartmentalization in the eukaryotic cells. The redox elements forming the "highly" and "widely" specialized redox system are found in all cell structures: mitochondria, plastids, peroxisomes, apoplast, nucleus etc. In recent years the active involvement of the central vacuole in the maintenance of the plant cell redox homeostasis is discussed, actually the information about the vacuolar redox system is very small. The high-priority redox processes and "redox-specialization" of the vacuolar compartment are not known. We have begun a study of red beet-root vacuole redox systems (Beta vulgaris L. and have identified redox enzymes such as: phenol peroxidase (EC 1.11.1.7, superoxide dismutase (EC 1.15.1.1 and glutathione reductase (EC 1.8.1.7. This paper presents some of the characteristics of these enzymes and considers the probable ways of their functioning in vacuolar redox chains.

  10. Chlorella vulgaris triggers apoptosis in hepatocarcinogenesis-induced rats

    Institute of Scientific and Technical Information of China (English)

    Emey Suhana MOHD AZAMAI; Suhaniza SULAIMAN; Shafina Hanim MOHD HABIB; Mee Lee LOOI; Srijit DAS; Nor Aini ABDUL HAMID; Wan Zurinah WANG NGAH; Yasmin Anum MOHD YUSOF

    2009-01-01

    Chlorella vulgaris (CV) has been reported to have antioxidant and anticancer properties. We evaluated the effect of CV on apoptotic regulator protein expression in liver cancer-induced rats. Male Wistar rats (200-250 g) were divided into eight groups: control group (normal diet), CDE group (choline deficient diet supplemented with ethionine in drinking water to induce hepatocarcinogenesis), CV groups with three different doses of CV (50, 150, and 300 mg/kg body weight), and CDE groups treated with different doses of CV (50, 150, and 300 mg/kg body weight). Rats were sacrificed at various weeks and liver tissues were embedded in paraffin blocks for immunohistochemistry studies. CV, at increasing doses, decreased the expression of anti-apoptotic protein, Bcl-2, but increased the expression of pro-apoptotic protein, caspase 8, in CDE rats, which was correlated with decreased hepatoctyes proliferation and increased apoptosis as determined by bromodeoxy-uridine (BrdU) labeling and terminal deoxynucleotidyl transferase mediated dUTP nick-end labeling (TUNEL) assay, respectively. Our study shows that CV has definite chemopreventive effect by inducing apoptosis via decreasing the expression of Bcl-2 and increasing the expression of caspase 8 in hepatocarcinogenesis-induced rats.

  11. Efficient in vitro micropropagation and regeneration of Artemisia vulgaris L.

    Directory of Open Access Journals (Sweden)

    Sujatha Govindaraj

    2007-01-01

    Full Text Available This paper describes efficient propagation of Artemisia vulgaris using shoot tip explants isolated from 35 daysold in vitro grown seedlings. Optimum proliferation was obtained on Murashige and Skoog’s salts and B5 vitamins supplementedwith 3% sucrose, 4.44 μM BA, and 0.7% agar. Shoot proliferation was maximal (99.8% with 14-23 shoots per explant after6 weeks of culture. Shoots with a minimum length of 1.5 cm were transferred to shoot elongation medium supplemented with0.44 μM BA and 1.44 μM GA3. The successfully elongated shoots with a height of 7.2-12.1 cm were transferred to rootingmedium augmented with 8.56 μM IAA. Rooted plantlets were transferred to plastic cups containing autoclaved garden soil,farmyard soil and sand (2:1:1 for hardening. Plantlets were initially maintained under culture room conditions (5 weeks,followed by normal laboratory conditions (4 weeks and finally transferred to a Botanical Evaluation Garden and maintainedthere.

  12. Inheritance of resistance to ozone in Phaseolus vulgaris L

    Energy Technology Data Exchange (ETDEWEB)

    Butler, L.K.; Tibbitts, T.W.; Bliss, F.A.

    1979-03-01

    Ozone sensitivity was compared in F/sub 1/ and F/sub 2/ populations from crosses between 2 ozone-sensitive bean cultivars, Spurt and Blue Lake Stringless, and 2 ozone-resistant cultivars, Black Turtle Soup and French's Horticultural, under controlled environmental conditions. F/sub 1/ plants were as sensitive as the sensitive parent. About 10% of the F/sub 2/ progeny obtained by selfing F/sub 1/ plants appeared to be as resistant as the resistant parent and 90% of the progeny could be divided equally between a group as sensitive as the sensitive parent and a group intermediate in sensitivity between the parent plants. However, precise separation of F/sub 2/ progeny was not possible because of the variability in injury expression. The average injury on the F/sub 2/ plants was greater than the parental midpoint value and the variance in injury on the F/sub 2/ plants was about 3.5x greater than that for the parents. The heritability of resistance to ozone was estimated to be 0.83. It was concluded that ozone resistance is recessive in P. vulgaris and appears to be regulated by a few major genes. 12 references, 2 figures, 2 tables.

  13. Systemic lupus erythematosus and pemphigus vulgaris: association or coincidence.

    Science.gov (United States)

    Calebotta, A; Cirocco, A; Giansante, E; Reyes, O

    2004-01-01

    Few cases have been published relating systemic lupus erythematosus (SLE) and pemphigus vulgaris (PV). We describe a patient with this association. A 35-year old woman who started to develop persistent pain and morning stiffness of proximal inter and metacarpo-phalangeal joints. During the following year, the patient recalled the onset of blisters on both legs, face, arms and thorax, as well as erosions appearing on oral mucous membranes. We observed generalized multiple erosions on her trunk and legs, flaccid bullae located on her right thigh and multiple erosions on oral mucous membranes. A skin biopsy reported PV Direct immunofluorescence on the perilesional skin specimen, showed beehive intercellular IgG deposits in the epidermis (+++), suggesting PV; granular discontinuous IgM and C3 deposits in the dermal-epidermal union (+++), suggesting SLE. Direct immunofluorescence of the healthy unexposed skin specimen, reported granular discontinuous IgG deposits in the dermal-epidermal union and beehive intercellular IgG deposits in the lower levels of the epidermis (+++); granular continuous IgM deposits in the dermal-epidermal union (+++). The results of rheumatic studies were obtained as follows: ANA :3 +, Anti-DNA, Anti-Sm, Anti-Ro and Anti-La :4 + . The definite diagnosis was PVand SLE. Treatment with 50 mg of prednisone daily with good evolution.

  14. Family study of monozygotic twins affected by pemphigus vulgaris.

    Science.gov (United States)

    Salathiel, Adriana Martinelli; Brochado, Maria José Franco; Kim, Olivia; Deghaide, Neifi Hassan Saloum; Donadi, Eduardo Antonio; Roselino, Ana Maria

    2016-07-01

    Rare are the family studies that include siblings affected by pemphigus vulgaris (PV) and in whom HLA class II alleles are related. HLA-DR and -DQ genotyping and profiling of antibodies against desmogleins (Dsg) 1 and Dsg3 were performed in ten members of a family including monozygotic twins affected by PV. The twin sisters were heterozygotes; they presented the haplotypes most commonly associated with increased susceptibility to PV (DRB1∗04:02-DQA1∗03:01-DQB1∗03:02 and DRB1∗14:04-DQA1∗01:01-DQB1∗05:03). Their parents and five siblings had only one or none of these two haplotypes in combination with the alleles or haplotypes associated with resistance to PV (DRB1∗07:01-DQA1∗02:01-DQB1∗02:02 and DRB1∗13:01-DQA1∗01:03-DQB1∗06:03). Only the monozygotic twins presented IgG antibodies against both Dsg1 and Dsg3. According to our knowledge based on a review of published literature on the topic, this is the first report of PV affecting monozygotic twins.

  15. Landscape genetics, adaptive diversity and population structure in Phaseolus vulgaris.

    Science.gov (United States)

    Rodriguez, Monica; Rau, Domenico; Bitocchi, Elena; Bellucci, Elisa; Biagetti, Eleonora; Carboni, Andrea; Gepts, Paul; Nanni, Laura; Papa, Roberto; Attene, Giovanna

    2016-03-01

    Here we studied the organization of genetic variation of the common bean (Phaseolus vulgaris) in its centres of domestication. We used 131 single nucleotide polymorphisms to investigate 417 wild common bean accessions and a representative sample of 160 domesticated genotypes, including Mesoamerican and Andean genotypes, for a total of 577 accessions. By analysing the genetic spatial patterns of the wild common bean, we documented the existence of several genetic groups and the occurrence of variable degrees of diversity in Mesoamerica and the Andes. Moreover, using a landscape genetics approach, we demonstrated that both demographic processes and selection for adaptation were responsible for the observed genetic structure. We showed that the study of correlations between markers and ecological variables at a continental scale can help in identifying local adaptation genes. We also located putative areas of common bean domestication in Mesoamerica, in the Oaxaca Valley, and the Andes, in southern Bolivia-northern Argentina. These observations are of paramount importance for the conservation and exploitation of the genetic diversity preserved within this species and other plant genetic resources.

  16. Plasma androgenic activity in women with acne vulgaris and in healthy girls before, during and after puberty.

    Science.gov (United States)

    Odlind, V; Carlström, K; Michaëlsson, G; Vahlqvist, A; Victor, A; Mellbin, T

    1982-03-01

    The possible relationship between plasma androgenic activity and acne vulgaris was investigated. Plasma testosterone and sex hormone binding globulin (SHBG) levels were determined in healthy girls during different stages of puberty, in healthy adult women and in women with acne vulgaris. Testosterone increase during puberty, whereas SHBG decreased during the early stages before it increased and stabilized plasma concentrations of testosterone and SHBG. Women with severe acne vulgaris had testosterone levels in the same range but the SHBG levels were significantly lower than those of healthy women and women with mild acne. These results show a high androgenic activity in the intermediate stages of puberty, when acne vulgaris is a common complaint and an increased androgenic activity in adult women with severe acne vulgaris.

  17. Lipid accumulation from pinewood pyrolysates by Rhodosporidium diobovatum and Chlorella vulgaris for biodiesel production.

    Science.gov (United States)

    Luque, Luis; Orr, Valerie C A; Chen, Sean; Westerhof, Roel; Oudenhoven, Stijn; Rossum, Guus van; Kersten, Sascha; Berruti, Franco; Rehmann, Lars

    2016-08-01

    This study evaluated the suitability of pinewood pyrolysates as a carbon source for lipid production and cultivation of the oleaginous yeast Rhodosporidium diobovatum and the microalgae Chlorella vulgaris. Thermal decomposition of pinewood and fractional condensation were used to obtain an oil rich in levoglucosan which was upgraded to glucose by acid hydrolysis. Blending of pyrolytic sugars with pure glucose in both nitrogen rich and nitrogen limited conditions was studied for R. diobovatum, and under nitrogen limited conditions for C. vulgaris. Glucose consumption rate decreased with increasing proportions of pyrolytic sugars increasing cultivation time. While R. diobovatum was capable of growth in 100% (v/v) pyrolytic sugars, C. vulgaris growth declined rapidly in blends greater than 20% (v/v) until no growth was detected in blends >40%. Finally, the effects of pyrolysis sugars on lipid composition was evaluated and biodiesel fuel properties were estimated based on the lipid profiles.

  18. Characterization and optimization of carbohydrate production from an indigenous microalga Chlorella vulgaris FSP-E.

    Science.gov (United States)

    Ho, Shih-Hsin; Huang, Shu-Wen; Chen, Chun-Yen; Hasunuma, Tomohisa; Kondo, Akihiko; Chang, Jo-Shu

    2013-05-01

    In this study, three indigenous microalgae isolates were examined for their ability to produce carbohydrates. Among them, Chlorella vulgaris FSP-E displayed relatively high cell growth rate and carbohydrate content. The carbohydrate productivity of C. vulgaris FSP-E was further improved by using engineering strategies. The results show that using an appropriate light intensity and inoculum size could effectively promote cell growth and carbohydrate productivity. Nitrogen starvation triggered the accumulation of carbohydrates in the microalga, achieving a carbohydrate content of 51.3% after 4-day starvation. Under the optimal conditions, the highest biomass and carbohydrate productivity were 1.437 and 0.631 g L(-1) d(-1), respectively. This performance is better than that reported in most related studies. Since glucose accounted for nearly 93% of the carbohydrates accumulated in C. vulgaris FSP-E, the microalga is an excellent feedstock for bioethanol fermentation.

  19. Caleosin from Chlorella vulgaris TISTR 8580 is salt-induced and heme-containing protein.

    Science.gov (United States)

    Charuchinda, Pairpilin; Waditee-Sirisattha, Rungaroon; Kageyama, Hakuto; Yamada, Daisuke; Sirisattha, Sophon; Tanaka, Yoshito; Mahakhant, Aparat; Takabe, Teruhiro

    2015-01-01

    Physiological and functional properties of lipid droplet-associated proteins in algae remain scarce. We report here the caleosin gene from Chlorella vulgaris encodes a protein of 279 amino acid residues. Amino acid sequence alignment showed high similarity to the putative caleosins from fungi, but less to plant caleosins. When the C. vulgaris TISTR 8580 cells were treated with salt stress (0.3 M NaCl), the level of triacylglycerol increased significantly. The mRNA contents for caleosin in Chlorella cells significantly increased under salt stress condition. Caleosin gene was expressed in E. coli. Crude extract of E. coli cells exhibited the cumene hydroperoxide-dependent oxidation of aniline. Absorption spectroscopy showed a peak around 415 nm which was decreased upon addition of cumene hydroperoxide. Native polyacrylamide gel electrophoresis suggests caleosin existed as the oligomer. These data indicate that a fresh water C. vulgaris TISTR 8580 contains a salt-induced heme-protein caleosin.

  20. In Vitro Antimicrobial Activity of Thymus vulgaris Essential Oil Against Major Oral Pathogens.

    Science.gov (United States)

    Fani, Mohammadmehdi; Kohanteb, Jamshid

    2017-01-01

    The objective of present investigation was to determine antimicrobial activity of Thymus vulgaris oil on some oral pathogens. Thymus vulgaris oil was prepared by hydrodistillation and tested against 30 clinical isolates of each of Streptococcus pyogenes, Streptococcus mutans, Candida albicans, Porphyromonas gingivalis, and Aggregatibacter actinomycetemcomitans, prepared from related oral infections using agar disk diffusion and broth microdilution methods. Thymus vulgaris oil at concentrations of 16 to 256 μg/mL exhibited strong inhibitory activity on all clinical isolates producing inhibition zones of 7.5 to 42 mm as measured by agar disk diffusion method. Streptococcus pyogenes and Streptococcus mutans were the most sensitive isolates with minimum inhibitory concentrations of 1.9 and 3.6 μg/mL, respectively. The minimum inhibitory concentration values for C albicans, A actinomycetemcomitans, and P gingivalis were 16.3, 32, and 32 μg/mL, respectively.

  1. Antispasmodic effect of hydroalcoholic extract of Thymus vulgaris on the guinea-pig ileum.

    Science.gov (United States)

    Babaei, Mehdi; Abarghoei, Mitra Emmami; Ansari, Reza; Vafaei, Abbas Ali; Taherian, Abbas Ali; Akhavan, Maziar Mohammad; Toussy, Gafar; Mousavi, Shahrokh

    2008-01-01

    The effects of Thymus vulgaris hydroalcoholic extract on the contractile responses of the isolated guinea-pig ileum were investigated. Contraction changes in the terminal ileum of guinea pigs were monitored using a force displacement transducer amplifier connected to a physiograph. Thymus vulgaris extract inhibited the contractile responses in a dose-dependent manner and also decreased the amplitude of peristaltic waves. It is concluded that T. vulgaris has an antispasmodic action on guinea pig ileum by decreasing the amplitudes of the muscle contractions during peristalsis. The EC50 was calculated as 1.7 mg mL(-1). In guinea-pig ileum the extract led to an antispasmodic effect, possibly by affecting the anticholinergic and serotoninergic pathways.

  2. Topical, Biological and Clinical Challenges in the Management of Patients with Acne Vulgaris

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    Anwar Al-Hammadi

    2016-05-01

    Full Text Available Acne vulgaris is one of the most common chronic inflammatory skin disorders among adolescents and young adults. It is associated with substantial morbidity and, rarely, with mortality. The exact worldwide incidence and prevalence are currently unknown. Current challenges involve improving understanding of the underlying pathophysiology of acne vulgaris and developing a practical treatment consensus. Expert panel discussions were held in 2013 and 2014 among a group of scientists and clinicians from the Omani and United Arab Emirate Dermatology Societies to ascertain the current optimal management of acne vulgaris, identify clinically relevant end-points and construct suitable methodology for future clinical trial designs. This article reviews the discussions of these sessions and recent literature on this topic.

  3. A comparative study of phytohaemagglutinin and extract of Phaseolus vulgaris seeds by characterization and cytogenetics

    Science.gov (United States)

    Badari Nath, A. R. S.; Sivaramakrishna, A.; Marimuthu, K. M.; Saraswathy, Radha

    2015-01-01

    Phytohaemagglutinin (PHA) is a lectin obtained from Phaseolus vulgaris (red kidney beans), that acts as a mitogen in human leucocyte culture and is commercially available from Gibco®. This PHA (Gibco®) was found to be very expensive, hence other inexpensive sources that can be used in all kinds of cytogenetics labs (rich and poor), were attempted. One such successful attempt was PHA extract from seeds of P.vulgaris. This paper details the methodology of extraction and application of PHA from seeds of P.vulgaris. Attempts has been made to identify the chemical and physical properties of the products in the extract, analyzed by various spectroscopic and analytical techniques. The analysis clearly indicates that the product from Phaseolus seeds extract was found to be similar to the commercially available PHA (Gibco®) in the cytogenetic study of human leucocyte cultures. The present study enforces the possible utility of the plant extract directly for human leucocyte cultures.

  4. Mangifera indica and Phaseolus vulgaris in the bioindication of air pollution in Bahia, Brazil.

    Science.gov (United States)

    Lima, J S; Fernandes, E B; Fawcett, W N

    2000-07-01

    In this article are reported the results of a study on the concentration of ascorbic acid (AA) in Mangifera indica as passive monitor and in Phaseolus vulgaris as active monitor with the intention to study the effects of industrial emissions from the Petrochemical Complex of Camaçari (PCC), Bahia, Brazil, on the vegetation. Leaves from M. indica were collected in two sites in the region under direct influence of industrial emissions and in one presumed nonpolluted reference (background) site. Pots with P. vulgaris were exposed in the same sites. The AA increase in the leaves of M. indica from PCC sites indicates a stress situation. The small AA increase in the P. vulgaris exposed in the more polluted site indicates that the active monitor is a sensitive one. The decrease of its leaf area indicates the inability of this species to activate physiological protection mechanisms like an increase in AA production.

  5. Clinical Observation on Effect of Triptolide Tablet in Treating Patients with Psoriasis Vulgaris

    Institute of Scientific and Technical Information of China (English)

    WU Shao-xi; GUO Ning-ru

    2005-01-01

    Objective: To investigate the effects of triptolide tablet in the treatment of patients with psoriasis vulgaris. Methods: By an open clinical study of 103 patients with psoriasis vulgaris. Psoriasis area severity index (PASI) was measured and recorded before and after treatment for efficacy evaluation. Results:Of the 103 patients, markedly effective was got in 41 (39.7%), improved in 37 (35.8%) and ineffective in 25 (24.5 % ), the total effective rate being 75.7%, and the adverse reaction was shown only in few patients with decreased WBC during the treatment period. Conclusion: Triptolide tablet is effective for the treatment of psoriasis vulgaris during the one-year follow-up.

  6. Antifungal activity of extracts of Rosmarinus officinalis and Thymus vulgaris against Aspergillus flavus and A. ochraceus.

    Science.gov (United States)

    Centeno, S; Calvo, M A; Adelantado, C; Figueroa, S

    2010-05-01

    The antifungal activity of ethanolic extracts of Rosmarinus officinalis and Thymus vulgaris were tested against strains of Aspergillus flavus and A. ochraceus, since these two species are common contaminants of cereals and grains and are able to produce and accumulate mycotoxins. The methodology used is based on measuring the inhibition halos produced by discs impregnated with the extracts and establishing their Minimum Inhibitory Concentration (MIC) as well as the Minimum Fungicide Concentration (MFC). The results obtained suggest that the assayed extracts affect the proper development of A. flavus and A. ochraceus; leading to a lower MIC (1200 ppm) and MFC (2400 ppm) for T. vulgaris extract against A. ochraceus than against A. flavus. The results show, that the extracts of Rosmarinus officinalis and Thymus vulgaris used at low concentrations could have significant potential for the biological control of fungi in foodstuffs.

  7. Removal Cu(II ions from water using sulphuric acid treated Lagenaria vulgaris Shell (Cucurbitaceae

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    Ljupković, R.B.

    2011-09-01

    Full Text Available Removal of Cu(II ions from water solutions by sulphuric acid treated Lagenaria vulgaris shell (ccLVB was studied. Batch experiments were done by shaking a fixed mass of biosorbent (1.0 g with 250 cm3 of 50.0 mg dm–3 Cu(II solutions, at pH ranged from 2 up to 6. Metal concentration in the filtrates as well as in the initial solution was determined by flame atomic absorption spectrometry. Results show that efficiency of Cu(II ions uptake by sulphuric acid treated Lagenaria vulgaris shell is significantly greater than raw Lagenaria vulgaris biosorbent. In addition, there is no significant effect of initial pH of solution on Cu(II ions uptake by ccLVB and obtained biosorbent can be applied in a wide range of pH.

  8. Topical, Biological and Clinical Challenges in the Management of Patients with Acne Vulgaris

    Science.gov (United States)

    Al-Hammadi, Anwar; Al-Ismaily, Abla; Al-Ali, Sameer; Ramadurai, Rajesh; Jain, Rishi; McKinley-Grant, Lynn; Mughal, Tariq I.

    2016-01-01

    Acne vulgaris is one of the most common chronic inflammatory skin disorders among adolescents and young adults. It is associated with substantial morbidity and, rarely, with mortality. The exact worldwide incidence and prevalence are currently unknown. Current challenges involve improving understanding of the underlying pathophysiology of acne vulgaris and developing a practical treatment consensus. Expert panel discussions were held in 2013 and 2014 among a group of scientists and clinicians from the Omani and United Arab Emirate Dermatology Societies to ascertain the current optimal management of acne vulgaris, identify clinically relevant end-points and construct suitable methodology for future clinical trial designs. This article reviews the discussions of these sessions and recent literature on this topic. PMID:27226905

  9. Topical, Biological and Clinical Challenges in the Management of Patients with Acne Vulgaris.

    Science.gov (United States)

    Al-Hammadi, Anwar; Al-Ismaily, Abla; Al-Ali, Sameer; Ramadurai, Rajesh; Jain, Rishi; McKinley-Grant, Lynn; Mughal, Tariq I

    2016-05-01

    Acne vulgaris is one of the most common chronic inflammatory skin disorders among adolescents and young adults. It is associated with substantial morbidity and, rarely, with mortality. The exact worldwide incidence and prevalence are currently unknown. Current challenges involve improving understanding of the underlying pathophysiology of acne vulgaris and developing a practical treatment consensus. Expert panel discussions were held in 2013 and 2014 among a group of scientists and clinicians from the Omani and United Arab Emirate Dermatology Societies to ascertain the current optimal management of acne vulgaris, identify clinically relevant end-points and construct suitable methodology for future clinical trial designs. This article reviews the discussions of these sessions and recent literature on this topic.

  10. Severe multi-resistant pemphigus vulgaris: prolonged remission with a single cycle of rituximab.

    Science.gov (United States)

    Corral, Isabela Soubhia; Freitas, Thais Helena Proença de; Aquino, Renata Telles Rudge de; Koller, Daniella Abbruzzini S; Magliari, Maria Elisa Ruffolo; Muller, Helena

    2013-01-01

    Pemphigus vulgaris is an autoimmune bullous disease whose therapy is based on systemic corticosteroids, with or without immunosuppressants. Rituximab is a chimeric monoclonal antibody of the IgG class, directed at a specific CD20 B cell surface antigen, used in pemphigus vulgaris empirically since 2002, with success in 90% of the cases and long periods of remission. Male patient, 33 years old, diagnosed with pemphigus vulgaris, confirmed by histopathology and direct immunofluorescence. He was treated for seven months with numerous treatments, including immunosuppressive drugs, with an unsatisfactory response, until he had complete remission with the use of rituximab. During a 34-month follow-up period, the patient presented a slight clinical relapse, which was successfully controlled with prednisone in a daily dose of 120 mg, soon reduced to 20mg.

  11. Influence of green algae Chlorella vulgaris on initial growth of different agricultural crops

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    Hajnal-Jafari Timea I.

    2016-01-01

    Full Text Available The aim of this research was to evaluate the effect of green algae Chlorella vulgaris on the initial growth of wheat, maize, bean and lettuce and the microbiological activity of rhizospheric soil. The experiment was conducted in controlled conditions. The inocula were applied as foliar fertilizer by spraying. Plant material was taken 30 days after plant emergance. Chlorella vulgaris affected positively the length (28.5% increase and fresh mass (17.9% increase of maize root, stem length of wheat (24.2% increase and stem mass of lettuce (56.34% increase. Application of Chlorella vulgaris led to the increase of the total number of bacteria and the number of aminoheterotrophs in the maize rhizosphere, total bacterial number in the wheat rhizosphere, and the number of fungi in the rhizosphere of bean. The number of other investigated groups of microorganisms did not change significantly. The activity of dehydrogenase enzyme was not affected by inoculation with green algae.

  12. [Effect of inorganic carbon source on lipid production with autotrophic Chlorella vulgaris].

    Science.gov (United States)

    Zheng, Hongli; Gao, Zhen; Zhang, Qi; Huang, He; Ji, Xiaojun; Sun, Honglei; Dou, Chang

    2011-03-01

    We studied the effects of three inorganic carbon sources, Na2CO3, NaHCO3 and CO2, and their initial concentrations on lipid production of Chlorella vulgaris. Chlorella vulgaris could utilize Na2CO3, NaHCO3 and CO2 to produce lipids. After 10-day cultivation with each of the three inorganic carbon sources, lipid yield of Chlorella vulgaris reached its peak with the concentration increase of the inorganic carbon source, but dropped again by further increase of the concentration. The pH value of the culture medium for Chlorella vulgaris increased after the cultivation on inorganic carbon source. The optimal concentration of both Na2CO3 and NaHCO3 was 40 mmol/L, and their corresponding biomass dry weight was 0.52 g/L and 0.67 g/L with their corresponding lipid yield 0.19 g/L and 0.22 g/L. When the concentration of CO2 was 6%, Chlorella vulgaris grew the fastest and its biomass dry weight was 2.42 g/L with the highest lipid yield of 0.72 g/L. When the concentration of CO2 was too low, the supply of inorganic carbon was insufficient and lipid yield was low. A too high concentration of CO2 caused a low pH and lipid accumulation was inhibited. Na2CO3 and NaHCO3 were more favorable for Chlorella vulgaris to accumulate unsaturated fatty acids than that of CO2.

  13. Toxicological Response of the Green Alga Chlorella vulgaris, to Some Heavy Metals

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    E. Afkar

    2010-01-01

    Full Text Available Problems statement: The disturbance of aquatic ecosystems provoked by heavy metals pollution from industrial and domestic sources, has as consequence the loss of biological diversity, as well as increased bioaccumulation and magnification of toxicants in the food chain. The aim of this study was to evaluate the effect of some heavy metals on some physiological activities of Chlorella vulgaris beyerinck with special references to metal bioaccumulation. Approach: Chlorella vulgaris Beyerinck was isolated from Al-Asfar Lake, Al-Hassa, Saudi Arabia. A standard initial inoculum of the isolated algae was inoculated to culture flasks. The culture flasks were supplied with various concentrations of Cobalt, Copper and Zinc ranging from 10-6-10-9 M. At the end of the incubation period cultures were filtered and washed several times by distilled water for measurements the various experimental parameters. Results: The data show that the lower doses of the three tested metals had stimulatory effect in biomass yield of Chlorella vulgaris, whereas the higher doses were inhibitory depending on the type of the metal. The inhibitory effect of copper to the growth parameters of Chlorella vulgaris was more pronounced than other two tested metals. The total protein content, total carbohydrate and the total free amino acids of the tested green alga Chlorella vulgaris gradually decreased in a manner dependent on the metal concentration in the medium. On the other hand, bioaccumulation of cobalt, copper and zinc by Chlorella vulgaris cells were parallel to increasing the concentrations in the culture medium. Conclusion: The inhibitory and stimulatory effects of either of the used heavy metals depend on concentration. Different organisms, however, have different sensitivities to the same metal and the same organisms may be more or less damaged by different metals. The uptake of an element from the surrounding medium is seldom exactly proportional to the amount present

  14. Inhibition of the catalase activity from Phaseolus vulgaris and Medicago sativa by sodium chloride.

    Science.gov (United States)

    Tejera García, Noel A; Iribarne, Carmen; Palma, Francisco; Lluch, Carmen

    2007-08-01

    Changes in catalase activity during the development of the Rhizobium-legume symbiosis as well as its response in salinized plants of Phaseolus vulgaris and Medicago sativa, was studied. Besides, it was examined the behavior of the enzyme, isolated from leaves and root nodules, during in vitro incubation with NaCl doses. Nodule catalase activities of both legumes were assayed with several enzyme inhibitors and also purified. Leaf catalase activity of Phaseolus vulgaris and Medicago sativa decreased and increased respectively throughout the ontogeny, but root nodule catalase kept a high and stable value. This last result suggests that both legumes require the maintenance of high nodule catalase in nitrogen-fixing nodules. Under salt stress conditions leaf and nodule catalase activity decreased in both, grain and pasture legumes. Because catalase from leaf of Medicago sativa and nodules of Phaseolus vulgaris were relatively sensitive to NaCl during in vitro experiments, the detoxifying role of this enzyme for H(2)O(2) should be limited in such conditions. Both catalases, from determinate and indeterminate nodules, were affected neither by oxygen nor superoxide radicals but showed a strong (Phaseolus vulgaris) or partial (Medicago sativa) inhibition with dithiothreitol, dithionite and beta-mercaptoethanol. Besides, cyanide was the most potent inhibitor of nodule catalases. Finally, catalases partially purified by immobilized metal ion affinity chromatography migrated at 42 (Phaseolus vulgaris) and 46kDa (Medicago sativa) on SDS-PAGE, whereas native forms on sephacryl S-300 columns exhibited a molecular mass of 59 and 48kDa (Phaseolus vulgaris) and 88 and 53kDa (Medicago sativa).

  15. Evaluation of leptin, adiponectin, and ghrelin levels in patients with acne vulgaris.

    Science.gov (United States)

    Ozuguz, P; Kacar, S D; Asik, G; Ozuguz, U; Karatas, S

    2016-02-09

    The research evaluating adipokines are very few in patients with acne vulgaris. The hypothesis that hyperinsulinemic and high glycemic index diet plays a role in the pathogenesis of acne is still controversial. In this study, we aimed to evaluate adipokines such as leptin (L), adiponectin (A), ghrelin and A levels, and A/L rates that indicate insulin resistance in nonobese patients with severe acne vulgaris. Thirty patients who are nonobese with moderate acne vulgaris, aged 18 to 25 years, and 15 age-sex compatible controls were included in our study. The acne lesions were assessed using the Global Acne Grading Scale (GAGS). All participants were evaluated for the parameters that may affect the metabolism of serum L, A, and ghrelin levels in blood, and their body mass index were calculated. The significance level was determined as p ≤ 0.05. Of the 30 patients, 17 were women and 13 were men. The mean age was 20.60 years and the mean duration of the disease were 2.8 years. All of patients had moderate acne vulgaris (GAGS 19-30). Of the 15 controls, 11 were women and 4 were men. The mean age was 21.20 years. There were not a statistically significant difference in L, ghrelin, A levels, and A/L ratio between the two groups. Adipokines may have a role in the pathogenesis of acne vulgaris. L, A, ghrelin, and insulin resistance may not participate in the responsible mechanisms in nonobese patients with moderate acne vulgaris. © The Author(s) 2016.

  16. The influence of aluminium availability on phosphate uptake in Phaseolus vulgaris L. and Phaseolus lunatus L.

    Science.gov (United States)

    Mimmo, Tanja; Sciortino, Marco; Ghizzi, Massimiliano; Gianquinto, Giorgio; Gessa, Carlo E

    2009-01-01

    Aluminium toxicity is one of the major limiting factors of crop productivity on acid soils. High levels of available aluminium in soil may induce phosphorus deficiency in plants. This study investigates the influence of Aluminium (Al) on the phosphate (P(i)) uptake of two Phaseolus species, Phaseolus vulgaris L. var. Red Kidney and Phaseolus lunatus L. The two bean species were treated first with solutions of Al at different concentrations (0, 25, 50 and 100microM, pH 4.50) and second with solutions of P(i) (150microM) at pH 4.50. The higher the Al concentration the higher the Al concentration sorbed but P. vulgaris L var. Red Kidney adsorbed significantly more Al than P. lunatus L. Both species released organic acids: P. vulgaris L var. Red Kidney released fumaric acid and P. lunatus L. fumaric and oxalic acids which could have hindered further Al uptake. The two bean species showed a sigmoid P(i) uptake trend but with two different mechanisms. P. vulgaris L var. Red Kidney showed a starting point of 3h whereas P. lunatus L. adsorbed P(i) immediately within the first minutes. In addition, P. vulgaris L var. Red Kidney presented significantly higher P(i) uptake (higher uptake rate 'k' and higher maximum adsorption 'a' of the kinetic uptake model). The Al treatments did not significantly influence P(i) uptake. Results suggest that P. lunatus L. might adopt an external Al detoxification mechanism by the release of oxalic acid. P. vulgaris L var. Red Kidney on the other hand seemed to adopt an internal detoxification mechanism even if the Al sorbed is poorly translocated into the shoots. More detailed studies will be necessary to better define Al tolerance and/or resistance of Phaseolus spp.

  17. PRESCRIPTION AUDIT OF ACNE VULGARIS IN SKIN OUTPATIENT DEPARTMENT OF A TERTIARY CARE TEACHING HOSPITAL

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    Vishal Prakash

    2014-10-01

    Full Text Available : OBJECTIVE: To evaluate prescribing pattern in acne vulgaris cases at a tertiary care teaching hospital in south India. METHODS: Prescriptions of 120 patients of acne vulgaris who attended Dermatology OPD of a tertiary care teaching hospital were selected for study and their drug data were analyzed. RESULTS: Topical Benzoyl peroxide, adapalene, ketoconazole were prescribed as monotherapy, while aloevera, liquid paraffin and white soft paraffin as polytherapy. Azithromycin, antibiotics, anti histaminics were prescribed as systemic monotherapy and polytherapy. Statistical analysis revealed p-value was > 0.05. CONCLUSIONS: Prescription patterns were in consensus with the general guidelines, with few changes, in the choice of established therapeutic agents.

  18. Antifungal activity of the essential oil of Thymus vulgaris L. and thymol on experimentally induced dermatomycoses.

    Science.gov (United States)

    Soković, M; Glamoclija, J; Cirić, A; Kataranovski, D; Marin, P D; Vukojević, J; Brkić, D

    2008-12-01

    The in vivo evaluation of the toxicological and antifungal activity of the essential oil of Thymus vulgaris L. and its main component thymol was made on 2-month-old male Wistar rats. We examined the therapeutic potency against experimentally induced dermatomycoses in rats, using the most frequent dermatomycetes, Trichophyton mentagrophytes, T. rubrum, and T. tonsurans. The therapeutic efficacy of a 1% solution of the essential oil of Thymus vulgaris and thymol as well as the commercial preparation bifonazole was evaluated. During the 37-day observation period the oil-treated animals were cured.

  19. Static and dynamic superheated water extraction of essential oil components from Thymus vulgaris L.

    Science.gov (United States)

    Dawidowicz, Andrzej L; Rado, Ewelina; Wianowska, Dorota

    2009-09-01

    Superheated water extraction (SWE) performed in both static and dynamic condition (S-SWE and D-SWE, respectively) was applied for the extraction of essential oil from Thymus vulgaris L. The influence of extraction pressure, temperature, time, and flow rate on the total yield of essential oil and the influence of extraction temperature on the extraction of some chosen components are discussed in the paper. The SWE extracts are related to PLE extracts with n-hexane and essential oil obtained by steam distillation. The superheated water extraction in dynamic condition seems to be a feasible option for the extraction of essential oil components from T. vulgaris L.

  20. Radiation-induced pemphigus vulgaris of the breast; Pemphigus vulgaire radio-induit du sein

    Energy Technology Data Exchange (ETDEWEB)

    Vigna-Taglianti, R.; Russi, E.G. [Department of radiotherapy, Santa Croce e Carle general hospital, Via M. Coppino, 12100 Cuneo (Italy); Denaro, N. [Oncology department, university of Messina, Via consolare Valeria no 1, 98100 Messina (Italy); Numico, G. [Department of medical oncology, U. Parini hospital, 11100 Aosta (Italy); Brizio, R. [Department of histopathology, Santa Croce e Carle general hospital, Via M. Coppino, 12100 Cuneo (Italy)

    2011-07-15

    Pemphigus vulgaris is a rare autoimmune muco-cutaneous bullous disease. Patients with a history of pemphigus vulgaris - who need radiotherapy - may show a long lasting bullous cutaneous manifestation, typical of pemphigus, within radiation fields. The literature describes fewer than 20 radio-induced cases. While systematic corticosteroid therapy has proven to be useful, topical treatment used in association with corticosteroid therapy is rarely described. To our knowledge the use of modern dressing products has never been described. We report our experience in a case in which modern dressing products were usefully associated to systemic therapy. (authors)