Sample records for vorticity vector derived

  1. Generalized Derivative Based Kernelized Learning Vector Quantization

    NARCIS (Netherlands)

    Schleif, Frank-Michael; Villmann, Thomas; Hammer, Barbara; Schneider, Petra; Biehl, Michael; Fyfe, Colin; Tino, Peter; Charles, Darryl; Garcia-Osoro, Cesar; Yin, Hujun


    We derive a novel derivative based version of kernelized Generalized Learning Vector Quantization (KGLVQ) as an effective, easy to interpret, prototype based and kernelized classifier. It is called D-KGLVQ and we provide generalization error bounds, experimental results on real world data, showing

  2. Fluid vortices

    National Research Council Canada - National Science Library

    Green, Sheldon I


    ... . . . . . . . . . . . . . . . Vorticity Kinematics and Dynamics - Physical Principles The Vorticity Equation with Examples . . . . Summary . . . . . . . . . . . . . . . . . Vorticity in Orthogonal...

  3. Design and implementation of a hot-wire probe for simultaneous velocity and vorticity vector measurements in boundary layers (United States)

    Zimmerman, S.; Morrill-Winter, C.; Klewicki, J.


    A multi-sensor hot-wire probe for simultaneously measuring all three components of velocity and vorticity in boundary layers has been designed, fabricated and implemented in experiments up to large Reynolds numbers. The probe consists of eight hot-wires, compactly arranged in two pairs of orthogonal ×-wire arrays. The ×-wire sub-arrays are symmetrically configured such that the full velocity and vorticity vectors are resolved about a single central location. During its design phase, the capacity of this sensor to accurately measure each component of velocity and vorticity was first evaluated via a synthetic experiment in a set of well-resolved DNS fields. The synthetic experiments clarified probe geometry effects, allowed assessment of various processing schemes, and predicted the effects of finite wire length and wire separation on turbulence statistics. The probe was subsequently fabricated and employed in large Reynolds number experiments in the Flow Physics Facility wind tunnel at the University of New Hampshire. Comparisons of statistics from the actual probe with those from the simulated sensor exhibit very good agreement in trend, but with some differences in magnitude. These comparisons also reveal that the use of gradient information in processing the probe data can significantly improve the accuracy of the spanwise velocity measurement near the wall. To the authors' knowledge, the present are the largest Reynolds number laboratory-based measurements of all three vorticity components in boundary layers.

  4. On derivation of BPS equations of vortices in K-generalized Abelian-Higgs model (United States)

    Atmaja, A. N.


    A new self-dual equation, or BPS equations, of vortices in the K-generalized Abelian-Higgs Model was derived by exploiting the identity equation of the scalar kinetic terms [1]. Here we develop a method for obtaining these BPS equations by assuming the BPS energy EBPS can be written as an integral over total derivative of energy function Q, which is a function of the effective fields, as such we can define a BPS Lagrangian ℒBPS ∝ -Q'(r), where r is an effective coordinate. Matching this BPS Lagrangian with the corresponding effective Lagrangian, we can extract the resulting BPS equations. We show there are two ways to get the BPS equations of vortices in the K-generalized Abelian-Higgs Model using our method.

  5. Discussion on the complete-form vorticity equation and slantwise vorticity development (United States)

    Wang, Xiuming; Zhou, Xiaogang; Tao, Zuyu; Liu, Hua


    The complete form of the vertical vorticity tendency equation (the complete-form vorticity equation) is derived from the Ertel potential vorticity equation to contain thermodynamic factors. In this study, a new complete-form vorticity equation, which has the same form as the original complete-form vorticity equation, is deduced from the absolute vorticity vector equation combined with the continuity equation and the expression of three-dimensional (3D) entropy gradient. By comparing the complete-form vorticity equation with the classical vertical vorticity equation, it is found that regardless of whether or not the isentropic surface is tilting, the two vorticity equations are in essence the same. The "baroclinic term" of the complete-form vorticity equation is exactly equal to the solenoidal term of the classical one, and there is a significant amount of cancellation between the two baroclinic items (the "slantwise term" and the horizontal vorticity change term) in the complete-form vorticity equation. In operational weather analysis, the tilt of the isentropic surface can be diagnosed according to the density of the isotherm on the upper-level isobaric map. For synoptic-scale motion, the vertical vorticity produced by the tilt of the isentropic surface is due to the contribution of atmospheric baroclinicity, which is measured by the solenoid. The 3D solenoid is parallel to the isentropic surface, so the more tilted the isentropic surface, the bigger the projection of the 3D solenoid in the vertical direction. The baroclinic contribution can be interpreted based on the PV thinking theory, but the relationship between the vorticity field and the potential vorticity field is not immediate.

  6. Chiral vortical effect for bosons (United States)

    Avkhadiev, Artur; Sadofyev, Andrey V.


    The thermal contribution to the chiral vortical effect is believed to be related to the axial anomaly in external gravitational fields. We use the universality of the spin-gravity interaction to extend this idea to a wider set of phenomena. We consider the Kubo formula at weak coupling for the spin current of a vector field and derive a novel anomalous effect caused by the medium rotation: the chiral vortical effect for bosons. The effect consists in a spin current of vector bosons along the angular velocity of the medium. We argue that it has the same anomalous nature as in the fermionic case and show that this effect provides a mechanism for helicity transfer, from flow helicity to magnetic helicity.

  7. Analytical Derivation of Three Dimensional Vorticity Function for wave breaking in Surf Zone


    Dutta, R.


    In this report, Mathematical model for generalized nonlinear three dimensional wave breaking equations was de- veloped analytically using fully nonlinear extended Boussinesq equations to encompass rotational dynamics in wave breaking zone. The three dimensional equations for vorticity distributions are developed from Reynold based stress equations. Vorticity transport equations are also developed for wave breaking zone. This equations are basic model tools for numerical simulation of surf zon...

  8. Global vorticity and the definition of the rotation of a deformable earth (United States)

    Georgiadou, P.; Grafarend, E.

    Within the framework of Newtonian kinematics the local vorticity vector is introduced and averaged with respect to global earth geometry, namely the ellipsoid of revolution. For a deformable body like the earth the global vorticity vector is defined as the earth rotation. A decomposition of the Lagrangian displacement and of the Lagrangian vorticity vector into vector spherical harmonics, namely into spheroidal and toroidal parts, proves that the global vorticity vector only contains toroidal coefficients of degree and order cone (polar motion) and toroidal coefficients of degree one and order zero (length of the day) in the case of an ellipsoidal earth. When an ellipsoid of revolution earth model is assumed, the earth rotation is also slightly dependent on the ellipsoidal flattering and the radial derivative of the spheroidal coefficients of degree two and order one. Finally these coefficients are computed for a Kelvin-type earth model under conservative external forces like tidal gravity.

  9. Vortical flows

    CERN Document Server

    Wu, Jie-Zhi; Zhou, Ming-De


    This book is a comprehensive and intensive book for graduate students in fluid dynamics as well as scientists, engineers and applied mathematicians. Offering a systematic introduction to the physical theory of vortical flows at graduate level, it considers the theory of vortical flows as a branch of fluid dynamics focusing on shearing process in fluid motion, measured by vorticity. It studies vortical flows according to their natural evolution stages,from being generated to dissipated. As preparation, the first three chapters of the book provide background knowledge for entering vortical flows. The rest of the book deals with vortices and vortical flows, following their natural evolution stages. Of various vortices the primary form is layer-like vortices or shear layers, and secondary but stronger form is axial vortices mainly formed by the rolling up of shear layers.  Problems are given at the end of each chapter and Appendix, some for helping understanding the basic theories, and some involving specific ap...

  10. Derivative self-interactions for a massive vector field

    Energy Technology Data Exchange (ETDEWEB)

    Beltrán Jiménez, Jose, E-mail: [CPT, Aix Marseille Université, UMR 7332, 13288 Marseille (France); Heisenberg, Lavinia, E-mail: [Institute for Theoretical Studies, ETH Zurich, Clausiusstrasse 47, 8092 Zurich (Switzerland)


    In this work we revisit the construction of theories for a massive vector field with derivative self-interactions such that only the 3 desired polarizations corresponding to a Proca field propagate. We start from the decoupling limit by constructing healthy interactions containing second derivatives of the Stueckelberg field with itself and also with the transverse modes. The resulting interactions can then be straightforwardly generalized beyond the decoupling limit. We then proceed to a systematic construction of the interactions by using the Levi–Civita tensors. Both approaches lead to a finite family of allowed derivative self-interactions for the Proca field. This construction allows us to show that some higher order terms recently introduced as new interactions trivialize in 4 dimensions by virtue of the Cayley–Hamilton theorem. Moreover, we discuss how the resulting derivative interactions can be written in a compact determinantal form, which can also be regarded as a generalization of the Born-Infeld lagrangian for electromagnetism. Finally, we generalize our results for a curved background and give the necessary non-minimal couplings guaranteeing that no additional polarizations propagate even in the presence of gravity.

  11. The effect of noise and sampling size on vorticity measurements in rotating fluids (United States)

    Wong, Kelvin K. L.; Kelso, Richard M.; Mazumdar, Jagannath; Abbott, Derek


    This paper describes a new technique for presenting information based on given flow images. Using a multistep first order differentiation technique, we are able to map in two dimensions, vorticity of fluid within a region of investigation. We can then present the distribution of this property in space by means of a color intensity map. In particular, the state of fluid rotation can be displayed using maps of vorticity flow values. The framework that is implemented can also be used to quantify the vortices using statistical properties which can be derived from such vorticity flow maps. To test our methodology, we have devised artificial vortical flow fields using an analytical formulation of a single vortex. Reliability of vorticity measurement from our results shows that the size of flow vector sampling and noise in flow field affect the generation of vorticity maps. Based on histograms of these maps, we are able to establish an optimised configuration that computes vorticity fields to approximate the ideal vortex statistically. The novel concept outlined in this study can be used to reduce fluctuations of noise in a vorticity calculation based on imperfect flow information without excessive loss of its features, and thereby improves the effectiveness of flow

  12. Effect of potential vorticity flux on the circulation in the South China Sea (United States)

    Zhu, Yaohua; Sun, Junchuan; Wang, Yonggang; Wei, Zexun; Yang, Dezhou; Qu, Tangdong


    This study analyzes temperature and salinity products from the U.S. Navy Generalized Digital Environment Model. To avoid the fictitious assumption of no-motion reference level, a P-vector inverse method is employed to derive geostrophic velocity. Line integral of geostrophic velocity shows evidence for the existence of a sandwiched circulation in the South China Sea (SCS), i.e., cyclonic circulation in the subsurface and deep layers and anticyclonic in the intermediate layer. To reveal the factors responsible for the sandwiched circulation, we derive the potential vorticity equation based on a four-and-a-half-layer quasi-geostrophic model and apply theoretical potential vorticity constraint to density layers. The result shows that the sandwiched circulation is largely induced by planetary potential vorticity flux through lateral boundaries, mainly the Luzon Strait. This dynamical mechanism lies in the fact that the net potential vorticity inflow in the subsurface and deep layers leads to a positive layer-average vorticity in the SCS basin, yielding vortex stretching and a cyclonic basin-wide circulation. On the contrary, the net potential vorticity outflow in the intermediate layer induces a negative layer-average vorticity, generating an anticyclonic basin-wide circulation in the SCS. Furthermore, by illustrating different consequence from depth/density layers, we clarify that density layers are essential for applying theoretical potential vorticity constraint to the isolated deep SCS basin.

  13. Compact vortices

    Energy Technology Data Exchange (ETDEWEB)

    Bazeia, D.; Losano, L.; Marques, M.A.; Zafalan, I. [Universidade Federal da Paraiba, Departamento de Fisica, Joao Pessoa, PB (Brazil); Menezes, R. [Universidade Federal da Paraiba, Departamento de Ciencias Exatas, Rio Tinto, PB (Brazil); Universidade Federal de Campina Grande, Departamento de Fisica, Campina Grande, PB (Brazil)


    We study a family of Maxwell-Higgs models, described by the inclusion of a function of the scalar field that represent generalized magnetic permeability. We search for vortex configurations which obey first-order differential equations that solve the equations of motion. We first deal with the asymptotic behavior of the field configurations, and then implement a numerical study of the solutions, the energy density and the magnetic field. We work with the generalized permeability having distinct profiles, giving rise to new models, and we investigate how the vortices behave, compared with the solutions of the corresponding standard models. In particular, we show how to build compact vortices, that is, vortex solutions with the energy density and magnetic field vanishing outside a compact region of the plane. (orig.)

  14. In vivo transduction of murine cerebellar Purkinje cells by HIV-derived lentiviral vectors. (United States)

    Torashima, Takashi; Okoyama, Shigeo; Nishizaki, Tomoyuki; Hirai, Hirokazu


    Cerebellar Purkinje cells are key elements in motor learning and motor coordination, and therefore, it is important to clarify the mechanisms by which Purkinje cells integrate information and control cerebellar function. Gene transfer into neurons, followed by the assessment of the effects on neural function, is an effective approach for examining gene function. However, this method has not been used fully in the study of the cerebellum because adenovirus vectors, the vectors most commonly used for in vivo gene transfer, have very low affinity for Purkinje cells. In this study, we used a human immunodeficiency virus (HIV)-derived lentiviral vector and examined the transduction profile of the vector in the cerebellum. A lentiviral vector carrying the GFP gene was injected into the cerebellar cortex. Seven days after the injection, Purkinje cells were efficiently transduced without significant influence on the cell viability and synaptic functions. GFP was also expressed, though less efficiently, in other cortical interneurons and Bergmann glias. In contrast to reported findings with other viral vectors, no transduced cells were observed outside of the cerebellar cortex. Thus, when HIV-derived lentiviral vectors were injected into the cerebellar cortex, transduction was limited to the cells in the cerebellar cortex, with the highest tropism for Purkinje cells. These results suggest that HIV-derived lentiviral vectors are useful for the study of gene function in Purkinje cells as well as for application as a gene therapy tool for the treatment of diseases that affect Purkinje cells.

  15. Helicity conservation and twisted Seifert surfaces for superfluid vortices (United States)

    Salman, Hayder


    Starting from the continuum definition of helicity, we derive from first principles its different contributions for superfluid vortices. Our analysis shows that an internal twist contribution emerges naturally from the mathematical derivation. This reveals that the spanwise vector that is used to characterize the twist contribution must point in the direction of a surface of constant velocity potential. An immediate consequence of the Seifert framing is that the continuum definition of helicity for a superfluid is trivially zero at all times. It follows that the Gauss-linking number is a more appropriate definition of helicity for superfluids. Despite this, we explain how a quasi-classical limit can arise in a superfluid in which the continuum definition for helicity can be used. This provides a clear connection between a microscopic and a macroscopic description of a superfluid as provided by the Hall-Vinen-Bekarevich-Khalatnikov equations. This leads to consistency with the definition of helicity used for classical vortices.

  16. Vectors

    DEFF Research Database (Denmark)

    Boeriis, Morten; van Leeuwen, Theo


    This article revisits the concept of vectors, which, in Kress and van Leeuwen’s Reading Images (2006), plays a crucial role in distinguishing between ‘narrative’, action-oriented processes and ‘conceptual’, state-oriented processes. The use of this concept in image analysis has usually focused...... on the most salient vectors, and this works well, but many images contain a plethora of vectors, which makes their structure quite different from the linguistic transitivity structures with which Kress and van Leeuwen have compared ‘narrative’ images. It can also be asked whether facial expression vectors...... should be taken into account in discussing ‘reactions’, which Kress and van Leeuwen link only to eyeline vectors. Finally, the question can be raised as to whether actions are always realized by vectors. Drawing on a re-reading of Rudolf Arnheim’s account of vectors, these issues are outlined...

  17. Primordial vorticity and gradient expansion

    CERN Document Server

    Giovannini, Massimo


    The evolution equations of the vorticities of the electrons, ions and photons in a pre-decoupling plasma are derived, in a fully inhomogeneous geometry, by combining the general relativistic gradient expansion and the drift approximation within the Adler-Misner-Deser decomposition. The vorticity transfer between the different species is discussed in this novel framework and a set of general conservation laws, connecting the vorticities of the three-component plasma with the magnetic field intensity, is derived. After demonstrating that a source of large-scale vorticity resides in the spatial gradients of the geometry and of the electromagnetic sources, the total vorticity is estimated to lowest order in the spatial gradients and by enforcing the validity of the momentum constraint. By acknowledging the current bounds on the tensor to scalar ratio in the (minimal) tensor extension of the $\\Lambda$CDM paradigm the maximal comoving magnetic field induced by the total vorticity turns out to be, at most, of the or...

  18. Adenovirus Vector-Derived VA-RNA-Mediated Innate Immune Responses

    Directory of Open Access Journals (Sweden)

    Hiroyuki Mizuguchi


    Full Text Available The major limitation of the clinical use of replication-incompetent adenovirus (Ad vectors is the interference by innate immune responses, including induction of inflammatory cytokines and interferons (IFN, following in vivo application of Ad vectors. Ad vector-induced production of inflammatory cytokines and IFNs also results in severe organ damage and efficient induction of acquired immune responses against Ad proteins and transgene products. Ad vector-induced innate immune responses are triggered by the recognition of Ad components by pattern recognition receptors (PRRs. In order to reduce the side effects by Ad vector-induced innate immune responses and to develop safer Ad vectors, it is crucial to clarify which PRRs and which Ad components are involved in Ad vector-induced innate immune responses. Our group previously demonstrated that myeloid differentiating factor 88 (MyD88 and toll-like receptor 9 (TLR9 play crucial roles in the Ad vector-induced inflammatory cytokine production in mouse bone marrow-derived dendritic cells. Furthermore, our group recently found that virus associated-RNAs (VA-RNAs, which are about 160 nucleotide-long non-coding small RNAs encoded in the Ad genome, are involved in IFN production through the IFN-β promoter stimulator-1 (IPS-1-mediated signaling pathway following Ad vector transduction. The aim of this review is to highlight the Ad vector-induced innate immune responses following transduction, especially VA-RNA-mediated innate immune responses. Our findings on the mechanism of Ad vector-induced innate immune responses should make an important contribution to the development of safer Ad vectors, such as an Ad vector lacking expression of VA-RNAs.

  19. Doppler vortography: a color Doppler approach for quantification of the intraventricular blood flow vortices (United States)

    Mehregan, Forough; Tournoux, François; Muth, Stéphan; Pibarot, Philippe; Rieu, Régis; Cloutier, Guy; Garcia, Damien


    We propose a new approach for quantification of intracardiac vorticity – Doppler vortography – based on conventional color Doppler images. Doppler vortography relies on the centrosymmetric properties of the vortices. Such properties induce particular symmetries in the Doppler flow data which can be exploited to describe the vortices quantitatively. For this purpose, a kernel filter was developed to derive a parameter –the blood vortex signature (BVS) – that allows detecting the main intracardiac vortices and estimating their core vorticities. The reliability of Doppler vortography was assessed in mock Doppler fields issued from simulations and in vitro data. Doppler vortography was also tested in patients and compared with vector flow mapping by echocardiography. Strong correlations were obtained between the Doppler vortography-derived and the ground-truth vorticities (in silico: r2 = 0.98, in vitro: r2 = 0.86, in vivo: r2 = 0.89). Our results demonstrated that Doppler vortography is a potentially promising echocardiographic tool for quantification of vortex flow in the left ventricle. PMID:24210865

  20. Doppler vortography: a color Doppler approach to quantification of intraventricular blood flow vortices. (United States)

    Mehregan, Forough; Tournoux, François; Muth, Stéphan; Pibarot, Philippe; Rieu, Régis; Cloutier, Guy; Garcia, Damien


    We propose a new approach to quantification of intracardiac vorticity based on conventional color Doppler images -Doppler vortography. Doppler vortography relies on the centrosymmetric properties of the vortices. Such properties induce particular symmetries in the Doppler flow data that can be exploited to describe the vortices quantitatively. For this purpose, a kernel filter was developed to derive a parameter, the blood vortex signature (BVS), that allows detection of the main intracardiac vortices and estimation of their core vorticities. The reliability of Doppler vortography was assessed in mock Doppler fields issued from simulations and in vitro data. Doppler vortography was also tested in patients and compared with vector flow mapping by echocardiography. Strong correlations were obtained between Doppler vortography-derived and ground-truth vorticities (in silico: r2 = 0.98, in vitro: r2 = 0.86, in vivo: r2 = 0.89). Our results indicate that Doppler vortography is a potentially promising echocardiographic tool for quantification of vortex flow in the left ventricle. Copyright © 2014 World Federation for Ultrasound in Medicine & Biology. Published by Elsevier Inc. All rights reserved.

  1. An XMRV Derived Retroviral Vector as a Tool for Gene Transfer

    Directory of Open Access Journals (Sweden)

    Rojas-Martinez Augusto


    Full Text Available Abstract Background Retroviral vectors are widely used tools for gene delivery and gene therapy. They are useful for gene expression studies and genetic manipulation in vitro and in vivo. Many retroviral vectors are derived from the mouse gammaretrovirus, murine leukemia virus (MLV. These vectors have been widely used in gene therapy clinical trials. XMRV, initially found in prostate cancer tissue, was the first human gammaretrovirus described. Findings We developed a new retroviral vector based on XMRV called pXC. It was developed for gene transfer to human cells and is produced by transient cotransfection of LNCaP cells with pXC and XMRV-packaging plasmids. Conclusions We demonstrated that pXC mediates expression of inserted transgenes in cell lines. This new vector will be a useful tool for gene transfer in human and non-human cell lines, including gene therapy studies.

  2. Vortices vacate vales and other singular tales (United States)

    Turner, Ari Mark

    Quantized vortices in superfluids are a microscopic analogue of the vortices found in a sink as the water rushes down the drain. Unlike ordinary vortices, vortices in superfluid helium or artificial Bose condensates are particle-like because they are very long-lived. My thesis discusses forces experienced by these vortices which have a geometric origin. The first part focuses on vortices in a layer of liquid helium on a curved substrate. Such vortices experience a force that has Gaussian curvature as its source; vortices are attracted to negative and repelled from positive Gaussian curvature. Ideas about contexts where this force might be observed experimentally and the conformal mapping techniques for calculating the strength of the force are presented. The second part focuses on predictions about vortices in a dilute optically trapped Bose condensate of spinor atoms and derives their properties from first principles. Now the atoms' internal spin space provides the nontrivial geometry; spinor states can be classified by introducing a set of points on the sphere called "spin roots". The spin two phase diagram is determined as a function of the interaction parameters. Homotopy theory shows that multiple types of vortices can be created in a condensate of spin two atoms. The interactions between these vortices can be calculated using the spin roots' symmetry group. Metastable vortex states can arise in both contexts. A vortex on a curved surface be trapped near a saddle point. If thermal fluctuations push the vortex away from the saddle, it can move to the edge of the film and disappear, releasing the kinetic energy of the superfluid currents that circle the vortex. Sets of vortices in a spinor condensate can trap each other in a molecule (in the presence of a magnetic field). After a long time, thermal fluctuations can bring the component molecules together, leading to a "chemical reaction" in which new vortices that are not trapped are produced. Then the molecule

  3. Vitality of optical vortices (Presentation)

    CSIR Research Space (South Africa)

    Roux, FS


    Full Text Available This presentation discusses the vitality of optical vortices to distinguish between vortex dipole creation and annihilation events. Vitality is expressed in terms of the transverse 1st and 2nd order derivatives of the optical field. It can be used...

  4. Chiral vortical effect from the compactified D4-branes with smeared D0-brane charge

    Energy Technology Data Exchange (ETDEWEB)

    Wu, Chao; Chen, Yidian [Institute of High Energy Physics, Chinese Academy of Sciences,Beijing 100049 (China); Huang, Mei [Institute of High Energy Physics, Chinese Academy of Sciences,Beijing 100049 (China); University of Chinese Academy of Sciences,Beijing 100049 (China); Theoretical Physics Center for Science Facilities, Chinese Academy of Sciences,Beijing 100049 (China)


    By using the boundary derivative expansion formalism of fluid/gravity correspondence, we study the chiral vortical effect from the compactified D4-branes with smeared D0-brane charge. This background corresponds to a strongly coupled, nonconformal relativistic fluid with a conserved vector current. The presence of the chiral vortical effect is induced by the addition of a Chern-Simons term in the bulk action. Except that the non-dissipative anomalous viscous coefficient and the sound speed rely only on the chemical potential, most of the other thermal and hydrodynamical quantities of the first order depend both on the temperature and the chemical potential. According to our result, the way that the chiral vortical effect coefficient depends on the chemical potential seems irrelevant with whether the relativistic fluid is conformal or not. Stability analysis shows that this anomalous relativistic fluid is stable and the doping of the smeared D0-brane charge will slow down the sound speed.

  5. Development of a gene silencing DNA vector derived from a broad host range geminivirus

    Directory of Open Access Journals (Sweden)

    Hancock Leandria C


    Full Text Available Abstract Background Gene silencing is proving to be a powerful tool for genetic, developmental, and physiological analyses. The use of viral induced gene silencing (VIGS offers advantages to transgenic approaches as it can be potentially applied to non-model systems for which transgenic techniques are not readily available. However, many VIGS vectors are derived from Gemini viruses that have limited host ranges. We present a new, unipartite vector that is derived from a curtovirus that has a broad host range and will be amenable to use in many non-model systems. Results The construction of a gene silencing vector derived from the geminivirus Beet curly top virus (BCTV, named pWSRi, is reported. Two versions of the vector have been developed to allow application by biolistic techniques or by agro-infiltration. We demonstrate its ability to silence nuclear genes including ribulose bisphosphate carboxylase small subunit (rbcS, transketolase, the sulfur allele of magnesium chelatase (ChlI, and two homeotic transcription factors in spinach or tomato by generating gene-specific knock-down phenotypes. Onset of phenotypes occurred 3 to 12 weeks post-inoculation, depending on the target gene, in organs that developed after the application. The vector lacks movement genes and we found no evidence for significant spread from the site of inoculation. However, viral amplification in inoculated tissue was detected and is necessary for systemic silencing, suggesting that signals generated from active viral replicons are efficiently transported within the plant. Conclusion The unique properties of the pWSRi vector, the ability to silence genes in meristem tissue, the separation of virus and silencing phenotypes, and the broad natural host range of BCTV, suggest that it will have wide utility.

  6. [RelB silencing in mouse bone-marrow derived dendritic cells mediated by lentiviral vector]. (United States)

    Bao, Jie; Wang, Qian; Zheng, Lei; Qiu, Yu-rong; Zeng, Fang-yin; Yang, Chun-li; Huang, Xian-zhang


    To silence RelB gene in mouse bone-marrow derived dendritic cells (DC) utilizing lentiviral vector, a novel tolerogenic dendritic cell with a relatively low expression level RelB was constructed and a new way to treat and prevent autoimmune diseases was explored. Interferential targeting sequence R5 of RelB in mice was designed, synthesized and cloned into lentiviral vectors. Together with viral packaging materials were co-cultured in 293FT cell line to package lentiviral vector. Supernatant fluids were harvested, then virus titer detected. Mouse bone marrow derived DCs were infected by lentivirus particle. RelB gene expression level was detected by RT-PCR and immunofluorescence staining and analyzed by software of geo pro. There are three experiment control groups including immature DC, mature DC and DC infected by a negative independent control of T6. A similar RelB expression was detected by RT-PCR and immunofluorescence staining assay between DC infected virus R5 and immature DC, but was lower than that of mature DC. Significant difference in statistics P < 0.05. A similar RelB expression was detected by RT-PCR and immunofluorescence staining approaches between DC infected virus T6 and mature DC, but was higher than that of immature DC. Significant difference in statistics P < 0.05. RelB gene expressed by mouse bone marrow derived DC was silenced by Lentivirus vector effectively. The lentivirus vector with a low immunogenicity can be used to immunotherapy in vivo and overcome difficult transfection problem of primary DC. A new viral vector of DC immunotherapy can be obtained.

  7. Magnetic gates and guides for superconducting vortices (United States)

    Vlasko-Vlasov, V. K.; Colauto, F.; Buzdin, A. I.; Rosenmann, D.; Benseman, T.; Kwok, W.-K.


    We image the motion of superconducting vortices in niobium film covered with a regular array of thin permalloy strips. By altering the magnetization orientation in the strips using a small in-plane magnetic field, we can tune the strength of interactions between vortices and the strip edges, enabling acceleration or retardation of the superconducting vortices in the sample and consequently introducing strong tunable anisotropy into the vortex dynamics. We discuss our observations in terms of the attraction/repulsion between point magnetic charges carried by vortices and lines of magnetic charges at the strip edges and derive analytical formulas for the vortex-magnetic strips coupling. Our approach demonstrates the analogy between the vortex motion regulated by the magnetic strip array and electric carrier flow in gated semiconducting devices. Scaling down the geometrical features of the proposed design may enable controlled manipulation of single vortices, paving the way for Abrikosov vortex microcircuits and memories.

  8. Measurements of vector-derived neurotrophic factor and green fluorescent protein levels in the brain. (United States)

    Klein, Ronald L; Hamby, Mary E; Sonntag, Christopher F; Millard, William J; King, Michael A; Meyer, Edwin M


    Demonstrating consistently reliable levels of expression is a critical part of any gene transfer study in order to assess variability and determine effective gene dosages. This article highlights some of the key methods for studying the expression levels of green fluorescent protein and neurotrophic factors after injections of adeno-associated virus (AAV) vectors into the brain. The data demonstrate greater spread and higher levels of expression using the cytomegalovirus/chicken beta-actin (CBA) promoter coupled with the woodchuck hepatitis virus posttranscriptional regulatory element (WPRE), compared to earlier AAV serotype 2 vectors. Injections of either CBA-nerve growth factor (NGF)-WPRE or CBA-glial cell line-derived neutrotrophic factor-WPRE AAV vectors into the nucleus basalis of the basal forebrain led to clear and consistent elevation of the respective trophic factor as measured by enzyme-linked immunosorbent assay, with NGF vectors affecting the size and number of cholinergic neurons. AAV serotype may also be important for the spread of expression, since injecting an AAV-5 vector into the hippocampus led to higher-frequency transfection of dentate gyrus granule neurons, suggesting altered tropism relative to AAV-2. Copyright 2002 Elsevier Science (USA)

  9. Development of Virus-Induced Gene Expression and Silencing Vector Derived from Grapevine Algerian Latent Virus. (United States)

    Park, Sang-Ho; Choi, Hoseong; Kim, Semin; Cho, Won Kyong; Kim, Kook-Hyung


    Grapevine Algerian latent virus (GALV) is a member of the genus Tombusvirus in the Tombusviridae and infects not only woody perennial grapevine plant but also herbaceous Nicotiana benthamiana plant. In this study, we developed GALV-based gene expression and virus-induced gene silencing (VIGS) vectors in N. benthamiana. The GALV coat protein deletion vector, pGMG, was applied to express the reporter gene, green fluorescence protein (GFP), but the expression of GFP was not detected due to the necrotic cell death on the infiltrated leaves. The p19 silencing suppressor of GALV was engineered to inactivate its expression and GFP was successfully expressed with unrelated silencing suppressor, HC-Pro, from soybean mosaic virus. The pGMG vector was used to knock down magnesium chelatase (ChlH) gene in N. benthamaina and the silencing phenotype was clearly observed on systemic leaves. Altogether, the GALV-derived vector is expected to be an attractive tool for useful gene expression and VIGS vectors in grapevine as well as N. benthamiana.

  10. Neighboring block based disparity vector derivation for multiview compatible 3D-AVC (United States)

    Kang, Jewon; Chen, Ying; Zhang, Li; Zhao, Xin; Karczewicz, Marta


    3D-AVC being developed under Joint Collaborative Team on 3D Video Coding (JCT-3V) significantly outperforms the Multiview Video Coding plus Depth (MVC+D) which simultaneously encodes texture views and depth views with the multiview extension of H.264/AVC (MVC). However, when the 3D-AVC is configured to support multiview compatibility in which texture views are decoded without depth information, the coding performance becomes significantly degraded. The reason is that advanced coding tools incorporated into the 3D-AVC do not perform well due to the lack of a disparity vector converted from the depth information. In this paper, we propose a disparity vector derivation method utilizing only the information of texture views. Motion information of neighboring blocks is used to determine a disparity vector for a macroblock, so that the derived disparity vector is efficiently used for the coding tools in 3D-AVC. The proposed method significantly improves a coding gain of the 3D-AVC in the multiview compatible mode about 20% BD-rate saving in the coded views and 26% BD-rate saving in the synthesized views on average.

  11. Vector dark energy models with quadratic terms in the Maxwell tensor derivatives

    Energy Technology Data Exchange (ETDEWEB)

    Haghani, Zahra; Shahidi, Shahab [Damghan University, School of Physics, Damghan (Iran, Islamic Republic of); Harko, Tiberiu [Babes-Bolyai University, Department of Physics, Cluj-Napoca (Romania); University College London, Department of Mathematics, London (United Kingdom); Sepangi, Hamid Reza [Shahid Beheshti University, Department of Physics, Tehran (Iran, Islamic Republic of)


    We consider a vector-tensor gravitational model with terms quadratic in the Maxwell tensor derivatives, called the Bopp-Podolsky term. The gravitational field equations of the model and the equations describing the evolution of the vector field are obtained and their Newtonian limit is investigated. The cosmological implications of a Bopp-Podolsky type dark energy term are investigated for a Bianchi type I homogeneous and anisotropic geometry for two models, corresponding to the absence and presence of the self-interacting potential of the field, respectively. The time evolutions of the Hubble function, of the matter energy density, of the shear scalar, of the mean anisotropy parameter, and of the deceleration parameter, respectively, as well as the field potentials are obtained for both cases by numerically integrating the cosmological evolution equations. In the presence of the vector type dark energy with quadratic terms in the Maxwell tensor derivatives, depending on the numerical values of the model parameters, the Bianchi type I Universe experiences a complex dynamical evolution, with the dust Universes ending in an isotropic phase. The presence of the self-interacting potential of the vector field significantly shortens the time interval necessary for the full isotropization of the Universe. (orig.)

  12. Glycoprotein is enough for sindbis virus-derived DNA vector to express heterogenous genes

    Directory of Open Access Journals (Sweden)

    Fu Juanjuan


    Full Text Available Abstract To investigate the necessity and potential application of structural genes for expressing heterogenous genes from Sindbis virus-derived vector, the DNA-based expression vector pVaXJ was constructed by placing the recombinant genome of sindbis-like virus XJ-160 under the control of the human cytomegalovirus (CMV promoter of the plasmid pVAX1, in which viral structural genes were replaced by a polylinker cassette to allow for insertion of heterologous genes. The defect helper plasmids pVaE or pVaC were developed by cloning the gene of glycoprotein E3E26KE1 or capsid protein of XJ-160 virus into pVAX1, respectively. The report gene cassette pVaXJ-EGFP or pV-Gluc expressing enhanced green fluorescence protein (EGFP or Gaussia luciferase (G.luc were constructed by cloning EGFP or G.luc gene into pVaXJ. EGFP or G.luc was expressed in the BHK-21 cells co-transfected with report gene cassettes and pVaE at levels that were comparable to those produced by report gene cassettes, pVaC and pVaE and were much higher than the levels produced by report gene cassette and pVaC, suggesting that glycoprotein is enough for Sindbis virus-derived DNA vector to express heterogenous genes in host cells. The method of gene expression from Sindbis virus-based DNA vector only co-transfected with envelop E gene increase the conveniency and the utility of alphavirus-based vector systems in general.

  13. Landslide displacement vectors derived from multi-temporal topographic LiDAR data (United States)

    Fey, Christine; Rutzinger, Martin; Bremer, Magnus; Prager, Christoph; Zangerl, Christian


    Information about slope geometry and kinematics of landslides is essential for hazard assessment, monitoring and planning of protection and mitigation measures. Especially for remote and inaccessible slopes, subsurface data (e.g. boreholes, tunnels, investigation adits) are often not available and thus the deformation characteristics must be derived from surface displacement data. In recent years, multi-temporal topographic LiDAR (Light Detection and Ranging) data became an increasingly improved tool for detecting topographic surface deformations. In this context, LiDAR-based change detection is commonly applied for quantifying surface elevation changes. Advanced change detection methods derive displacement vectors with direction and velocities of slope movements. To extract displacement vectors from LiDAR raster data (i) an approach based on feature tracking by image correlation and (ii) an approach based on feature tracking by vectors breaklines are investigated. The image correlation method is based on the IMCORR software (National Snow and Ice Data Center, University of Colorado, Boulder), implemented in a SAGA GIS module. The image correlation algorithm is based on a normalized cross-covariance method. The algorithm searches tie points in two feature rasters derived from a digital surface model acquired at different time stamps. The method assesses automatically the displacement rates and directions of distinct terrain features e.g. displaced mountain ridges or striking boulders. In contrast the vector-based breakline methods require manual selection of tie points. The breaklines are the product of vectorized curvature raster images and extracting the "upper terrain edges" (topographic ridges) and "lower terrain edges" (topographic depressions). Both methods were tested on simulated terrain with determined displacement rates in order to quantify i) the accuracy ii) the minimum detectable movement rates iii) the influence of terrain characteristics iv) the

  14. Vorticity and helicity in relativistic heat-conducting fluid (United States)

    Prasad, G.


    The evolution of heat-conducting fluid described by a pair of Maxwell-like equations is used to construct thermal-fluid helicity and thermal-helicity currents. These currents are found to be dissipative. It is shown that the magnetic part of the particle vorticity two-form is a thermal-fluid vorticity flux vector field composed of a linear combination of the fluid’s vorticity and a spacelike twist of heat flow lines. Heat flow lines are non-geodesic because of the interplay between gravitation and the entropy entrainment in a system composed of a heat-conducting fluid which is in state of rapid differential rotation and far from equilibrium. In general, alignment of the heat flux vector with that of the fluid’s vorticity leads to non-conservation of thermal-fluid vorticity flux in both a thermal-fluid flux tube and a stream tube. It is demonstrated that the twist of the fluid’s vortex lines is caused by the heat flow along the fluid’s vorticity vector in the case of an axisymmetric stationary differentially rotating heat-conducting fluid configuration. In this case, dissipation of thermal-fluid vorticity flux along the flux tube is caused by coupled effects of the fluid’s vorticity magnitude, thermal resistivity and entropy entrainment.

  15. Efficient Model Order Reduction for the Dynamics of Nonlinear Multilayer Sheet Structures with Trial Vector Derivatives

    Directory of Open Access Journals (Sweden)

    Wolfgang Witteveen


    Full Text Available The mechanical response of multilayer sheet structures, such as leaf springs or car bodies, is largely determined by the nonlinear contact and friction forces between the sheets involved. Conventional computational approaches based on classical reduction techniques or the direct finite element approach have an inefficient balance between computational time and accuracy. In the present contribution, the method of trial vector derivatives is applied and extended in order to obtain a-priori trial vectors for the model reduction which are suitable for determining the nonlinearities in the joints of the reduced system. Findings show that the result quality in terms of displacements and contact forces is comparable to the direct finite element method but the computational effort is extremely low due to the model order reduction. Two numerical studies are presented to underline the method’s accuracy and efficiency. In conclusion, this approach is discussed with respect to the existing body of literature.

  16. Gene transfer by retrovirus-derived shuttle vectors in the generation of murine bispecific monoclonal antibodies. (United States)

    DeMonte, L B; Nistico, P; Tecce, R; Dellabona, P; Momo, M; Anichini, A; Mariani, M; Natali, P G; Malavasi, F


    The present study reports on the use of gene transfer by retrovirus-derived shuttle vectors in the generation of hybrid hybridomas secreting bispecific monoclonal antibodies. neo- and dhfr- genes were infected into distinct murine hybridomas, thus conferring a dominant resistance trait to geneticin (G418) and to methotrexate. The vectors employed were replication-deficient and dependent on complementation by a helper virus provided by the irradiated packaging lines. After cocultivation with the relevant packaging cell lines, stable hybridoma lines expressing the selectable markers were easily obtained and were then suitable for conventional somatic fusion. This high-efficiency method was used to generate two bispecific monoclonal antibodies simultaneously targeting molecules expressed on cytotoxic cells (i.e., T lymphocytes and natural killer cells) against a human melanoma-associated antigen. Images PMID:2326256

  17. Design and application of cationic amphiphilic β-cyclodextrin derivatives as gene delivery vectors (United States)

    Wan, Ning; Huan, Meng-Lei; Ma, Xi-Xi; Jing, Zi-Wei; Zhang, Ya-Xuan; Li, Chen; Zhou, Si-Yuan; Zhang, Bang-Le


    The nano self-assembly profiles of amphiphilic gene delivery vectors could improve the density of local cationic head groups to promote their DNA condensation capability and enhance the interaction between cell membrane and hydrophobic tails, thus increasing cellular uptake and gene transfection. In this paper, two series of cationic amphiphilic β-cyclodextrin (β-CD) derivatives were designed and synthesized by using 6-mono-OTs-β-CD (1) as the precursor to construct amphiphilic gene vectors with different building blocks in a selective and controlled manner. The effect of different type and degree of cationic head groups on transfection and the endocytic mechanism of β-CD derivatives/DNA nanocomplexes were also investigated. The results demonstrated that the designed β-cyclodextrin derivatives were able to compact DNA to form stable nanocomplexes and exhibited low cytotoxicity. Among them, PEI-1 with PEI head group showed enhanced transfection activity, significantly higher than commercially available agent PEI25000 especially in the presence of serum, showing potential application prospects in clinical trials. Moreover, the endocytic uptake mechanism involved in the gene transfection of PEI-1 was mainly through caveolae-mediated endocytosis, which could avoid the lysosomal degradation of loaded gene, and had great importance for improving gene transfection activity.

  18. Gradient and vorticity banding

    NARCIS (Netherlands)

    Dhont, Jan K.G.; Briels, Willem J.


    "Banded structures" of macroscopic dimensions can be induced by simple shear flow in many different types of soft matter systems. Depending on whether these bands extend along the gradient or vorticity direction, the banding transition is referred to as "gradient banding" or "vorticity banding,"


    Directory of Open Access Journals (Sweden)

    B. L. Artamonov


    Full Text Available The linear vortical model ot the final scope of a wing is exsamined. It representis the flat rectangular spatial veil covered with continuously distributed vortical layer. Elements of digitization of a veil are the quadrangular panels laying on its surface. Method, algorithms and the program of calculation of three making vectors of inductive speed from any guided rectangular platform covered with a vortical layer are created. Its intensity linearly changes on the surface of a platform. The decision is received in elementary functions. The numerical way solves the task of a definition of the law of circulation of the attached whirlwinds in scope of a wing and calculation of its aerodynamic characteristics, being based on the accepted vortical model and a hypothesis of flat sections.

  20. Initial Circulation and Peak Vorticity Behavior of Vortices Shed from Airfoil Vortex Generators (United States)

    Wendt, Bruce J.; Biesiadny, Tom (Technical Monitor)


    An extensive parametric study of vortices shed from airfoil vortex generators has been conducted to determine the dependence of initial vortex circulation and peak vorticity on elements of the airfoil geometry and impinging flow conditions. These elements include the airfoil angle of attack, chord length, span, aspect ratio, local boundary layer thickness, and free stream Mach number. In addition, the influence of airfoil-to-airfoil spacing on the circulation and peak vorticity has been examined for pairs of co-rotating and counter-rotating vortices. The vortex generators were symmetric airfoils having a NACA-0012 cross-sectional profile. These airfoils were mounted either in isolation, or in pairs, on the surface of a straight pipe. The turbulent boundary layer thickness to pipe radius ratio was about 17 percent. The circulation and peak vorticity data were derived from cross-plane velocity measurements acquired with a seven-hole probe at one chord-length downstream of the airfoil trailing edge location. The circulation is observed to be proportional to the free-stream Mach number, the angle-of-attack, and the span-to-boundary layer thickness ratio. With these parameters held constant, the circulation is observed to fall off in monotonic fashion with increasing airfoil aspect ratio. The peak vorticity is also observed to be proportional to the free-stream Mach number, the airfoil angle-of-attack, and the span-to-boundary layer thickness ratio. Unlike circulation, however, the peak vorticity is observed to increase with increasing aspect ratio, reaching a peak value at an aspect ratio of about 2.0 before falling off again at higher values of aspect ratio. Co-rotating vortices shed from closely spaced pairs of airfoils have values of circulation and peak vorticity under those values found for vortices shed from isolated airfoils of the same geometry. Conversely, counter-rotating vortices show enhanced values of circulation and peak vorticity when compared to values

  1. Vorticity and vortex dynamics

    CERN Document Server

    Wu, Jie-Zhi; Zhou, M-D


    The importance of vorticity and vortex dynamics has now been well rec- nized at both fundamental and applied levels of ?uid dynamics, as already anticipatedbyTruesdellhalfcenturyagowhenhewrotethe?rstmonograph onthesubject, The Kinematics of Vorticity(1954);andasalsoevidencedby the appearance of several books on this ?eld in 1990s. The present book is characterizedbythefollowingfeatures: 1. A basic physical guide throughout the book. The material is directed by a basic observation on the splitting and coupling of two fundamental processes in ?uid motion, i.e., shearing (unique to ?uid) and compre- ing/expanding.Thevorticityplaysakeyroleintheformer,andavortex isnothingbuta?uidbodywithhighconcentrationofvorticitycompared to its surrounding ?uid. Thus, the vorticity and vortex dynamics is - cordinglyde?nedasthetheoryofshearingprocessanditscouplingwith compressing/expandingprocess. 2. A description of the vortex evolution following its entire life.Thisbegins from the generation of vorticity to the formation of thi...

  2. Gyrofluid potential vorticity equation and turbulent equipartion states

    DEFF Research Database (Denmark)

    Madsen, Jens; Juul Rasmussen, Jens; Naulin, Volker


    An equation governing potential vorticity in a magnetized plasmas is derived. The equation is analogous to Ertel's theorem. In the long wave-length limit the potential vorticity equals the ratio of the gyro-frequency plus the E × B- and diamagnetic polarization densities to the particle density...

  3. Magneto-static vortices in two dimensional Abelian gauge theories


    Bellazzini, J.; Bonanno, C.; Siciliano, G


    We study the existence of vortices of the Klein-Gordon-Maxwell equations in the two dimensional case. In particular we find sufficient conditions for the existence of vortices in the magneto-static case, i.e when the electric potential $\\phi=0$. This result, due to the lack of suitable embedding theorems for the vector potential $\\A$ is achieved with the help of a penalization method.

  4. Structures of the vorticity tube segment in turbulence (United States)

    Wang, Lipo


    To address the geometrical properties of the turbulent velocity vector field, a new concept named streamtube segment has been developed recently [L. Wang, "On properties of fluid turbulence along streamlines," J. Fluid Mech. 648, 183-203 (2010), 10.1017/S0022112009993041]. According to the vectorial topology, the entire velocity field can be partitioned into the so-called streamtube segments, which are organized in a non-overlapping and space-filling manner. In principle, properties of turbulent fields can be reproduced from those of the decomposed geometrical units with relatively simple structures. A similar idea is implemented to study the turbulent vorticity vector field using the vorticity tube segment structure. Differently from the conventional vortex tubes, vorticity tube segments are space-filling and can be characterized by non-arbitrary parameters, which enables a more quantitative description rather than just an illustrative explanation of turbulence behaviors. From analyzing the direct numerical simulation data, the topological and dynamical properties of vorticity tube segments are explored. The characteristic parameters have strong influence on some conditional statistics, such as the enstrophy production and the probability density function of vorticity stretching. Consequently the common knowledge in turbulence dynamics that vorticity are more stretched than compressed need to be rectified in the vorticity tube segment context.

  5. Unravelling effects of flavanols and their derivatives on acrylamide formation via support vector machine modelling. (United States)

    Huang, Mengmeng; Wang, Qiao; Chen, Xinyu; Zhang, Yu


    This study investigated the effect of flavanols and their derivatives on acrylamide formation under low-moisture conditions via prediction using the support vector regression (SVR) approach. Acrylamide was generated in a potato-based equimolar asparagine-reducing sugar model system through oven heating. Both positive and negative effects were observed when the flavonoid treatment ranged 1-10,000μmol/L. Flavanols and derivatives (100μmol/L) suppress the acrylamide formation within a range of 59.9-78.2%, while their maximal promotion effects ranged from 2.15-fold to 2.84-fold for the control at a concentration of 10,000μmol/L. The correlations between inhibition rates and changes in Trolox-equivalent antioxidant capacity (ΔTEAC) (RTEAC-DPPH=0.878, RTEAC-ABTS=0.882, RTEAC-FRAP=0.871) were better than promotion rates (RTEAC-DPPH=0.815, RTEAC-ABTS=0.749, RTEAC-FRAP=0.841). Using ΔTEAC as variables, an optimized SVR model could robustly serve as a new predictive tool for estimating the effect (R: 0.783-0.880), the fitting performance of which was slightly better than that of multiple linear regression model (R: 0.754-0.880). Copyright © 2016 Elsevier Ltd. All rights reserved.

  6. Orientation and circulation of vortices in a turbulent boundary layer (United States)

    Gao, Qi; Ortiz-Dueñas, Cecilia; Longmire, Ellen


    The strengths of individual vortices are important in determining the generation and development of surrounding vortices in turbulent boundary layers. The dual-plane PIV data at z^+ = 110 and z/δ = 0.53 in a turbulent boundary layer at Reτ=1160 obtained by Ganapathisubramani et al. (2006) were investigated. 3D swirl strength was used to identify vortex cores. The eigenvector of the velocity gradient tensor was used to determine the orientation of each core, and the resulting eigenvector direction was compared with the average vorticity direction. Circulation of the cores was calculated using the vorticity vector only and using the vorticity vector projected onto the eigenvector. The probability distribution of the angle between the eigenvector and the vorticity vector indicated a peak at 15-20 degrees. The eigenvector angle distributions indicate that at z^+=110, more hairpin legs cross the measurement plane while at z/δ = 0.53, more heads are evident. Details of the orientation and circulation distributions will be discussed in the presentation.

  7. Vortices at Microwave Frequencies (United States)

    Silva, Enrico; Pompeo, Nicola; Dobrovolskiy, Oleksandr V.


    The behavior of vortices at microwave frequencies is an extremely useful source of information on the microscopic parameters that enter the description of the vortex dynamics. This feature has acquired particular relevance since the discovery of unusual superconductors, such as cuprates. Microwave investigation then extended its field of application to many families of superconductors, including the artificially nanostructured materials. It is then important to understand the basics of the physics of vortices moving at high frequency, as well as to understand what information the experiments can yield (and what they can not). The aim of this brief review is to introduce the readers to some basic aspects of the physics of vortices under a microwave electromagnetic field, and to guide them to an understanding of the experiment, also by means of the illustration of some relevant results.

  8. In vitro and in vivo Functional Characterization of Gutless Recombinant SV40-derived CFTR Vectors (United States)

    Mueller, Christian; Strayer, Marlene S; Sirninger, Jeffery; Braag, Sofia; Branco, Francisco; Louboutin, Jean-Pierre; Flotte, Terence R.; Strayer, David S.


    In cystic fibrosis (CF) respiratory failure caused by progressive airway obstruction and tissue damage is primarily a result of the aberrant inflammatory responses to lung infections with Pseudomonas aeruginosa. Despite considerable improvement in patient survival, conventional therapies are mainly supportive. Recent progress towards gene therapy for CF has been encouraging; however, several factors such as immune response and transduced cell turnover remain as potential limitations to CF gene therapy. As alternative gene therapy vectors for CF we examined the feasibility of using SV40-derived vectors (rSV40s) which may circumvent some of these obstacles. To accommodate the large CFTR cDNA, we removed not only SV40 Tag genes, but also all capsid genes. We therefore tested whether “gutless” rSV40s could be packaged and were able to express a functional human CFTR cDNA. Results from our in vitro analysis determined that rSV40-CFTR was able to successfully result in the expression of CFTR protein which localized to the plasma membrane and restored channel function to CFTR deficient cells. Similarly in vivo experiments delivering rSV40-CFTR to the lungs of Cftr−/− mice resulted in a reduction of the pathology associated with intra-tracheal pseudomona aeruginosa challenge. rSV40-CFTR treated mice had had less weight loss when compared to control treated mice as well as demonstrably reduced lung inflammation as evidence by histology and reduced inflammatory cytokines in the BAL. The reduction in inflammatory cytokine levels led to an evident decrease in neutrophil influx to the airways. These results indicate that further study of the application of rSV40-CFTR to CF gene therapy is warranted. PMID:19890354

  9. Theory of Concentrated Vortices

    DEFF Research Database (Denmark)

    Alekseenko, Sergey; Kuibin, Pavel; Okulov, Valery

    This book presents comprehensive and authoritative coverage of the wide field of concentrated vortices observed in nature and technique. The methods for research of their kinematics and dynamics are considered. Special attention is paid to the flows with helical symmetry. The authors have describ...... models of vortex structures used for interpretation of experimental data which serve as a ground for development of theoretical and numerical approaches to vortex investigation. Achievements in the fields of stability analysis, waves on vortices and vortex breakdown are also presented....

  10. Transformation of Cowpea Vigna unguiculata Cells with an Antibiotic Resistance Gene Using a Ti-Plasmid-Derived Vector

    NARCIS (Netherlands)

    Hille, Jacques; Goldbach, Rob


    A chimaeric antibiotic resistance gene was transferred to cowpea (Vigna unguiculata), a member of the legume family. This transfer was established by inoculating cowpea leaf discs with an Agrobacterium tumefaciens strain harboring a Ti-plasmid-derived vector that contained two copies of a chimaeric

  11. Stability of periodic arrays of vortices

    CERN Document Server

    Dauxois, T; Tuckerman, L S; Dauxois, Thierry; Fauve, Stephan; Tuckerman, Laurette


    The stability of periodic arrays of Mallier-Maslowe or Kelvin-Stuart vortices is discussed. We derive with the energy-Casimir stability method the nonlinear stability of this solution in the inviscid case as a function of the solution parameters and of the domain size. We exhibit the maximum size of the domain for which the vortex street is stable. By adapting a numerical time-stepping code, we calculate the linear stability of the Mallier-Maslowe solution in the presence of viscosity and compensating forcing. Finally, the results are discussed and compared to a recent experiment in fluids performed by Tabeling et al.~[Europhysics Letters {\\bf 3}, 459 (1987)]. Electromagnetically driven counter-rotating vortices are unstable above a critical electric current, and give way to co-rotating vortices. The importance of the friction at the bottom of the experimental apparatus is also discussed.

  12. Vortices and Jacobian varieties

    DEFF Research Database (Denmark)

    Manton, Nicholas S.; M. Romão, Nuno


    We investigate the geometry of the moduli space of N-vortices on line bundles over a closed Riemann surface of genus g > 1, in the little explored situation where 1 = 1, the vortex metric typically degenerates as the dissolving limit is approached, the degeneration occurring precisely on the crit...

  13. Relativistic Electron Vortices. (United States)

    Barnett, Stephen M


    The desire to push recent experiments on electron vortices to higher energies leads to some theoretical difficulties. In particular the simple and very successful picture of phase vortices of vortex charge ℓ associated with ℓℏ units of orbital angular momentum per electron is challenged by the facts that (i) the spin and orbital angular momentum are not separately conserved for a Dirac electron, which suggests that the existence of a spin-orbit coupling will complicate matters, and (ii) that the velocity of a Dirac electron is not simply the gradient of a phase as it is in the Schrödinger theory suggesting that, perhaps, electron vortices might not exist at a fundamental level. We resolve these difficulties by showing that electron vortices do indeed exist in the relativistic theory and show that the charge of such a vortex is simply related to a conserved orbital part of the total angular momentum, closely related to the familiar situation for the orbital angular momentum of a photon.

  14. Infectious Clones and Vectors Derived from Adeno-Associated Virus (AAV) Serotypes Other Than AAV Type 2


    Rutledge, Elizabeth A.; Halbert, Christine L.; Russell, David W.


    Adeno-associated viruses (AAVs) are single-stranded dependent parvoviruses being developed as transducing vectors. Although at least five serotypes exist (AAV types 1 to 5 [AAV1 to -5]), only AAV2, AAV3, and AAV4 have been sequenced, and the vectors in use were almost all derived from AAV2. Here we report the cloning and sequencing of a second AAV3 genome and a new AAV serotype designated AAV6 that is related to AAV1. AAV2, AAV3, and AAV6 were 82% identical at the nucleotide sequence level, a...

  15. On the evaluation of vorticity using cardiovascular magnetic resonance velocity measurements. (United States)

    Garcia, J; Larose, E; Pibarot, P; Kadem, L


    Vorticity and vortical structures play a fundamental role affecting the evaluation of energetic aspects (mainly left ventricle work) of cardiovascular function. Vorticity can be derived from cardiovascular magnetic resonance (CMR) imaging velocity measurements. However, several numerical schemes can be used to evaluate the vorticity field. The main objective of this work is to assess different numerical schemes used to evaluate the vorticity field derived from CMR velocity measurements. We compared the vorticity field obtained using direct differentiation schemes (eight-point circulation and Chapra) and derivate differentiation schemes (Richardson 4* and compact Richardson 4*) from a theoretical velocity field and in vivo CMR velocity measurements. In all cases, the effect of artificial spatial resolution up-sampling and signal-to-noise ratio (SNR) on vorticity computation was evaluated. Theoretical and in vivo results showed that the eight-point circulation method underestimated vorticity. Up-sampling evaluation showed that the artificial improvement of spatial resolution had no effect on mean absolute vorticity estimation but it affected SNR for all methods. The Richardson 4* method and its compact version were the most accurate and stable methods for vorticity magnitude evaluation. Vorticity field determination using the eight-point circulation method, the most common method used in CMR, has reduced accuracy compared to other vorticity schemes. Richardson 4* and its compact version showed stable SNR using both theoretical and in vivo data.

  16. Virus-derived gene expression and RNA interference vector for grapevine. (United States)

    Kurth, Elizabeth G; Peremyslov, Valera V; Prokhnevsky, Alexey I; Kasschau, Kristin D; Miller, Marilyn; Carrington, James C; Dolja, Valerian V


    The improvement of the agricultural and wine-making qualities of the grapevine (Vitis vinifera) is hampered by adherence to traditional varieties, the recalcitrance of this plant to genetic modifications, and public resistance to genetically modified organism (GMO) technologies. To address these challenges, we developed an RNA virus-based vector for the introduction of desired traits into grapevine without heritable modifications to the genome. This vector expresses recombinant proteins in the phloem tissue that is involved in sugar transport throughout the plant, from leaves to roots to berries. Furthermore, the vector provides a powerful RNA interference (RNAi) capability of regulating the expression of endogenous genes via virus-induced gene-silencing (VIGS) technology. Additional advantages of this vector include superb genetic capacity and stability, as well as the swiftness of technology implementation. The most significant applications of the viral vector include functional genomics of the grapevine and disease control via RNAi-enabled vaccination against pathogens or invertebrate pests.

  17. Vortices and nanostructured superconductors

    CERN Document Server


    This book provides expert coverage of modern and novel aspects of the study of vortex matter, dynamics, and pinning in nanostructured and multi-component superconductors. Vortex matter in superconducting materials is a field of enormous beauty and intellectual challenge, which began with the theoretical prediction of vortices by A. Abrikosov (Nobel Laureate). Vortices, vortex dynamics, and pinning are key features in many of today’s human endeavors: from the huge superconducting accelerating magnets and detectors at the Large Hadron Collider at CERN, which opened new windows of knowledge on the universe, to the tiny superconducting transceivers using Rapid Single Flux Quanta, which have opened a revolutionary means of communication. In recent years, two new features have added to the intrinsic beauty and complexity of the subject: nanostructured/nanoengineered superconductors, and the discovery of a range of new materials showing multi-component (multi-gap) superconductivity. In this book, leading researche...

  18. Can plastic bag derived-microplastics act as vectors for metal exposure in terrestrial invertebrates? (United States)

    E Hodson, Mark; Duffus-Hodson, Calum A.; Prendergast-Miller, Miranda; Thorpe, Karen


    Microplastics are widely reported contaminants in marine and freshwater ecosystems and studies have shown that they can be ingested by aquatic organisms and lead to potential negative effects on health. The effects can arise from the physical effects of the plastics (e.g. food displacement and blockages of the digestive tract) and from their potential to adsorb contaminants, primarily organic compounds, resulting in an increased exposure of the organism to toxic contaminants. Studies are beginning to emerge that also show a high abundance of microplastics in the terrestrial environment but there remains a lack of data on the impacts of these terrestrial microplastics or their interaction with other terrestrial pollutants. We conducted Zn adsorption experiments using HDPE microplastics, derived from plastic bags. Zinc adsorption to microplastics was similar to that observed in soils, but in the presence of both soil and microplastics, preferential adsorption onto the soil was observed. In desorption experiments, desorption of Zn from microplastics and soils was minimal (microplastics compared to 2 - 15 % for the soils. In earthworm exposure experiments Lumbricus terrestris earthworms cultivated in soils containing 0.35% by mass of Zn-bearing plastic (236 - 4505 mg kg-1) ingested the microplastics with no evidence for either preferential feeding or avoidance. There was no evidence for an accumulation of the microplastics in the earthworm gut or for signs of toxicity. Our experiments demonstrate that earthworms will ingest microplastics and that microplastics can adsorb metals and act as vectors for metal exposure in soil invertebrates. However, for Zn, the risk associated with this exposure appears to be minimal.

  19. NOAA Composite Shoreline - Vectorized Shoreline Derived From NOAA-NOS Coastal Survey Maps and Aerial Photographs (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The NOAA Composite Shoreline is primarily intended for high-resolution cartographic representation of the shoreline. It is a high-resolution vector shoreline based...


    National Aeronautics and Space Administration — This data set contains the Magellan Global Vector Data Record (GVDR), a sorted collection of scattering and emission measurements from the Magellan Mission. The...

  1. Vorticity budget investigation of a simulated long-lived mesoscale vortex in South China (United States)

    Chen, Min; Zheng, Yongguang


    A vorticity budget investigation is performed using the output data from a numerical simulation of a typical MCV (mesoscale convectively generated votex) case in South China. Results suggest that the divergence caused by convection in the low troposphere is the main producer of positive vorticity, while vertical vorticity transferred by the tilting term from the horizontal vorticity compensates the upward output of cyclonic vorticity. Scale analyses of the vorticity equation suggest that the advection of planetary vorticity can be neglected owing to the low latitude, which is different from the larger scale systems in high latitude areas. In addition, the distribution of relative vorticity tendency on pressure level is not uniform. A vortex will move along the vector from the negative to the positive vorticity tendency region. The mechanism of the phenomenon—that nearly all of the convectively ascending region is located southward/southeastward of the vortex center—is also discussed. Convergence with regard to latent heat release would be in favor of the spin-up of meso-vortex, however, the horizontal vorticity caused by wind shear is tilted by vertical motion due to convection. Consequently, the negative and positive vorticity tendencies are located symmetrically about the convective center, which suggests that the vortex southward movement is dynamically driven by convection.

  2. Dissecting zero modes and bound states on BPS vortices in Ginzburg-Landau superconductors

    Energy Technology Data Exchange (ETDEWEB)

    Izquierdo, A. Alonso [Departamento de Matematica Aplicada, Universidad de Salamanca,Facultad de Ciencias Agrarias y Ambientales,Av. Filiberto Villalobos 119, E-37008 Salamanca (Spain); Fuertes, W. Garcia [Departamento de Fisica, Universidad de Oviedo, Facultad de Ciencias,Calle Calvo Sotelo s/n, E-33007 Oviedo (Spain); Guilarte, J. Mateos [Departamento de Fisica Fundamental, Universidad de Salamanca, Facultad de Ciencias,Plaza de la Merced, E-37008 Salamanca (Spain)


    In this paper the zero modes of fluctuation of cylindrically symmetric self-dual vortices are analyzed and described in full detail. These BPS topological defects arise at the critical point between Type II and Type I superconductors, or, equivalently, when the masses of the Higgs particle and the vector boson in the Abelian Higgs model are equal. In addition, novel bound states of Higss and vector bosons trapped by the self-dual vortices at their core are found and investigated.

  3. Dynamics of nonstationary dipole vortices

    DEFF Research Database (Denmark)

    Hesthaven, J.S.; Lynov, Jens-Peter; Nycander, J.


    The dynamics of tilted dipole vortices in the equivalent barotropic vorticity (or Hasegawa-Mima) equation is studied. A recent theory is compared with numerical simulations and found to describe the short time behavior of dipole vortices well. In the long time limit the dipoles are found to either...... disintegrate or relax toward a steady eastward propagating dipole vortex. This relaxation is a consequence of nonviscous enstrophy loss by the dipole vortex....

  4. Sharp asymptotic estimates for vorticity solutions of the 2D Navier-Stokes equation

    Directory of Open Access Journals (Sweden)

    Yuncheng You


    Full Text Available The asymptotic dynamics of high-order temporal-spatial derivatives of the two-dimensional vorticity and velocity of an incompressible, viscous fluid flow in $mathbb{R}^2$ are studied, which is equivalent to the 2D Navier-Stokes equation. It is known that for any integrable initial vorticity, the 2D vorticity solution converges to the Oseen vortex. In this paper, sharp exterior decay estimates of the temporal-spatial derivatives of the vorticity solution are established. These estimates are then used and combined with similarity and $L^p$ compactness to show the asymptotical attraction rates of temporal-spatial derivatives of generic 2D vorticity and velocity solutions by the Oseen vortices and velocity solutions respectively. The asymptotic estimates and the asymptotic attraction rates of all the derivatives obtained in this paper are independent of low or high Reynolds numbers.

  5. On the definition of a moist-air potential vorticity

    CERN Document Server

    Marquet, Pascal


    A new potential vorticity is derived by using a specific entropy formulation expressed in terms of a moist-air entropy potential temperature. The new formulation is compared with Ertel's version and with others based on virtual and equivalent potential temperatures. The new potential vorticity is subject to conservative properties ensured by the Second Law applied to the moist-air material derivatives. It is shown that the upper tropospheric and stratospheric (dry) structures are nearly the same as those obtained with Ertel's component. Moreover, new structures are observed in the low troposphere, with negative values associated with moist frontal regions. The negative values are observed in the frontal regions where slantwise convection instabilities may take place, but they are smaller than those observed with the equivalent potential vorticity. The main purpose of the article is to diagnose the behaviour of the new potential vorticity from numerical output generated by the ARPEGE NWP model, with the help o...

  6. Efficient transduction of equine adipose-derived mesenchymal stem cells by VSV-G pseudotyped lentiviral vectors. (United States)

    Petersen, Gayle F; Hilbert, Bryan; Trope, Gareth; Kalle, Wouter; Strappe, Padraig


    Equine adipose-derived mesenchymal stem cells (EADMSC) provide a unique cell-based approach for treatment of a variety of equine musculoskeletal injuries, via regeneration of diseased or damaged tissue, or the secretion of immunomodulatory molecules. These capabilities can be further enhanced by genetic modification using lentiviral vectors, which provide a safe and efficient method of gene delivery. We investigated the suitability of lentiviral vector technology for gene delivery into EADMSC, using GFP expressing lentiviral vectors pseudotyped with the G glycoprotein from the vesicular stomatitis virus (V-GFP) or, for the first time, the baculovirus gp64 envelope protein (G-GFP). In this study, we produced similarly high titre V-GFP and G-GFP lentiviral vectors. Flow cytometric analysis showed efficient transduction using V-GFP; however G-GFP exhibited a poor ability to transduce EADMSC. Transduction resulted in sustained GFP expression over four passages, with minimal effects on cell viability and doubling time, and an unaltered chondrogenic differentiation potential. Copyright © 2014 Elsevier Ltd. All rights reserved.

  7. Analysis of scalar dissipation in terms of vorticity geometry in isotropic turbulence

    CERN Document Server

    Gonzalez, Michel


    The mechanisms promoting scalar dissipation through scalar gradient production are scrutinized in terms of vorticity alignment with respect to strain principal axes. For that purpose, a stochastic Lagrangian model for the velocity gradient tensor and the scalar gradient vector is used. The model results show that the major part of scalar dissipation occurs for stretched vorticity, namely when the vorticity vector aligns with the extensional and intermediate strain eigenvectors. More specifically, it appears that the mean scalar dissipation is well represented by the sample defined by alignment with the extensional strain, while the most intense scalar dissipation is promoted by the set of events for which vorticity aligns with the intermediate strain. This difference is explained by rather subtle mechanisms involving the statistics of both the strain intensities and the scalar gradient alignment resulting from these special alignments of vorticity. The analysis allowing for the local flow structure confirms t...

  8. Ginzburg-Landau vortices

    CERN Document Server

    Bethuel, Fabrice; Helein, Frederic


    This book is concerned with the study in two dimensions of stationary solutions of uɛ of a complex valued Ginzburg-Landau equation involving a small parameter ɛ. Such problems are related to questions occurring in physics, e.g., phase transition phenomena in superconductors and superfluids. The parameter ɛ has a dimension of a length which is usually small.  Thus, it is of great interest to study the asymptotics as ɛ tends to zero. One of the main results asserts that the limit u-star of minimizers uɛ exists. Moreover, u-star is smooth except at a finite number of points called defects or vortices in physics. The number of these defects is exactly the Brouwer degree – or winding number – of the boundary condition. Each singularity has degree one – or as physicists would say, vortices are quantized. The singularities have infinite energy, but after removing the core energy we are lead to a concept of finite renormalized energy.  The location of the singularities is completely determined by minimiz...

  9. Derivation of Double-Targeted Adenovirus Vectors for Gene Therapy of Prostate Cancer (United States)


    targeted phage vector fuCT/C6C/hrGFP. Forty-eight hours post-transduction the cells were examined under the fluorescent microscope . Left panel...fluorescent image; right panel, the same field viewed under white light. No fluorecent foci could be seen in a similarly transduced monolayer of parental

  10. Boundaries of the origin of replication: creation of a pET-28a-derived vector with p15A copy control allowing compatible coexistence with pET vectors.

    Directory of Open Access Journals (Sweden)

    Sarmitha Sathiamoorthy

    Full Text Available During our studies involving protein-DNA interactions, we constructed plasmid pSAM to fulfill two requirements: 1 to facilitate transfer of cloned sequences from widely used expression vector pET-28a(+, and 2 to provide a vector compatible with pBR322-derived plasmids for use in cells harboring two different plasmids. Vector pSAM is a pET-28a(+-derived plasmid with the p15A origin of replication (ori; pET-28a(+ contains the pBR322 replicon that is incompatible with other pBR322-derived plasmids. By replacing the original pET-28a(+ replicon-comprising the ori, RNAI, RNAII, and Rom-with the p15A replicon, we generated pSAM, which contains the pET-28a(+ multiple cloning site and is now compatible with pBR322-derived vectors. Plasmid copy number was assessed using quantitative PCR: pSAM copy number was maintained at 18±4 copies per cell, consistent with that of other p15A-type vectors. Compatibility with pBR322-derived vectors was tested with pGEX-6p-1 and pSAM, which maintained their copy numbers of 49±10 and 14±4, respectively, when both were present within the same cell. Swapping of the ori is a common practice; however, it is vital that all regions of the original replicon be removed. Additional vector pSAMRNAI illustrated that incompatibility remains when portions of the replicon, such as RNAI and/or Rom, are retained; pSAMRNAI, which contains the intact RNAI but not ROM, lowered the copy number of pGEX-6p-1 to 18±2 in doubly transformed cells due to retention of the pET-28a(+-derived RNAI. Thus, pSAMRNAI is incompatible with vectors controlled by the pBR322 replicon and further demonstrates the need to remove all portions of the original replicon and to quantitatively assess copy number, both individually and in combination, to ensure vector compatibility. To our knowledge, this is the first instance where the nascent vector has been quantitatively assessed for both plasmid copy number and compatibility. New vector pSAM provides ease of

  11. Human adipose derived mesenchymal stromal cells transduced with GFP lentiviral vectors: assessment of immunophenotype and differentiation capacity in vitro. (United States)

    van Vollenstee, Fiona A; Jackson, Carlo; Hoffmann, Danie; Potgieter, Marnie; Durandt, Chrisna; Pepper, Michael S


    Adipose derived mesenchymal stromal/stem cells (ASCs) are a heterogeneous population characterized by (a) their ability to adhere to plastic; (b) immunophenotypic expression of certain cell surface markers, while lacking others; and (c) the capacity to differentiate into lineages of mesodermal origin including osteocytes, chondrocytes and adipocytes. The long-term goal is to utilize these cells for clinical translation into cell-based therapies. However, preclinical safety and efficacy need to be demonstrated in animal models. ASCs can also be utilized as biological vehicles for vector-based gene delivery systems, since they are believed to home to sites of inflammation and infection in vivo. These factors motivated the development of a labelling system for ASCs using lentiviral vector-based green fluorescent protein (GFP) transduction. Human ASCs were transduced with GFP-expressing lentiviral vectors. A titration study determined the viral titer required to transduce the maximum number of ASCs. The effect of the transduced GFP lentiviral vector on ASC immunophenotypic expression of surface markers as well as their ability to differentiate into osteocytes and adipocytes were assessed in vitro. A transduction efficiency in ASC cultures of approximately 80 % was observed with an MOI of ~118. No significant immunophenotypic differences were observed between transduced and non-transduced cells and both cell types successfully differentiated into adipocytes and osteocytes in vitro. We obtained >80 % transduction of ASCs using GFP lentiviral vectors. Transduced ASCs maintained plastic adherence, demonstrated ASC immunophenotype and the ability to differentiate into cells of the mesodermal lineage. This GFP-ASC transduction technique offers a potential tracking system for future pre-clinical studies.

  12. A herpes simplex virus-derived replicative vector expressing LIF limits experimental demyelinating disease and modulates autoimmunity. (United States)

    Nygårdas, Michaela; Paavilainen, Henrik; Müther, Nadine; Nagel, Claus-Henning; Röyttä, Matias; Sodeik, Beate; Hukkanen, Veijo


    Herpes simplex virus type 1 (HSV-1) has properties that can be exploited for the development of gene therapy vectors. The neurotropism of HSV enables delivery of therapeutic genes to the nervous system. Using a bacterial artificial chromosome (BAC), we constructed an HSV-1(17(+))-based replicative vector deleted of the neurovirulence gene γ134.5, and expressing leukemia inhibitory factor (LIF) as a transgene for treatment of experimental autoimmune encephalomyelitis (EAE). EAE is an inducible T-cell mediated autoimmune disease of the central nervous system (CNS) and is used as an animal model for multiple sclerosis. Demyelination and inflammation are hallmarks of both diseases. LIF is a cytokine that has the potential to limit demyelination and oligodendrocyte loss in CNS autoimmune diseases and to affect the T-cell mediated autoimmune response. In this study SJL/J mice, induced for EAE, were treated with a HSV-LIF vector intracranially and the subsequent changes in disease parameters and immune responses during the acute disease were investigated. Replicating HSV-LIF and its DNA were detected in the CNS during the acute infection, and the vector spread to the spinal cord but was non-virulent. The HSV-LIF significantly ameliorated the EAE and contributed to a higher number of oligodendrocytes in the brains when compared to untreated mice. The HSV-LIF therapy also induced favorable changes in the expression of immunoregulatory cytokines and T-cell population markers in the CNS during the acute disease. These data suggest that BAC-derived HSV vectors are suitable for gene therapy of CNS disease and can be used to test the therapeutic potential of immunomodulatory factors for treatment of EAE.

  13. A novel, broad-range, CTXΦ-derived stable integrative expression vector for functional studies. (United States)

    Das, Bhabatosh; Kumari, Reena; Pant, Archana; Sen Gupta, Sourav; Saxena, Shruti; Mehta, Ojasvi; Nair, Gopinath Balakrish


    CTXΦ, a filamentous vibriophage encoding cholera toxin, uses a unique strategy for its lysogeny. The single-stranded phage genome forms intramolecular base-pairing interactions between two inversely oriented XerC and XerD binding sites (XBS) and generates a functional phage attachment site, attP(+), for integration. The attP(+) structure is recognized by the host-encoded tyrosine recombinases XerC and XerD (XerCD), which enables irreversible integration of CTXΦ into the chromosome dimer resolution site (dif) of Vibrio cholerae. The dif site and the XerCD recombinases are widely conserved in bacteria. We took advantage of these conserved attributes to develop a broad-host-range integrative expression vector that could irreversibly integrate into the host chromosome using XerCD recombinases without altering the function of any known open reading frame (ORF). In this study, we engineered two different arabinose-inducible expression vectors, pBD62 and pBD66, using XBS of CTXΦ. pBD62 replicates conditionally and integrates efficiently into the dif of the bacterial chromosome by site-specific recombination using host-encoded XerCD recombinases. The expression level of the gene of interest could be controlled through the PBAD promoter by modulating the functions of the vector-encoded transcriptional factor AraC. We validated the irreversible integration of pBD62 into a wide range of pathogenic and nonpathogenic bacteria, such as V. cholerae, Vibrio fluvialis, Vibrio parahaemolyticus, Escherichia coli, Salmonella enterica, and Klebsiella pneumoniae. Gene expression from the PBAD promoter of integrated vectors was confirmed in V. cholerae using the well-studied reporter genes mCherry, eGFP, and lacZ. Copyright © 2014, American Society for Microbiology. All Rights Reserved.

  14. Interaction of plasma vortices with resonant particles

    DEFF Research Database (Denmark)

    Jovanovic, D.; Pécseli, Hans; Juul Rasmussen, J.


    Kinetic effects associated with the electron motion along magnetic field lines in low‐beta plasmas are studied. Using the gyrokinetic description of electrons, a kinetic analog of the reduced magnetohydrodynamic equations is derived, and it is shown that in the strongly nonlinear regime they poss......Kinetic effects associated with the electron motion along magnetic field lines in low‐beta plasmas are studied. Using the gyrokinetic description of electrons, a kinetic analog of the reduced magnetohydrodynamic equations is derived, and it is shown that in the strongly nonlinear regime...... particles. The evolution equations indicate the possibility of excitation of plasma vortices by electron beams....

  15. Gateway-compatible transposon vector to genetically modify human embryonic kidney and adipose-derived stromal cells. (United States)

    Petrakis, Spyros; Raskó, Tamas; Mátés, Lajos; Ivics, Zoltan; Izsvák, Zsuzsanna; Kouzi-Koliakou, Kokkona; Koliakos, George


    The Gateway technology cloning system and transposon technology represent state-of-the-art laboratory techniques. Combination of these molecular tools allows rapid cloning of target genes into expression vectors. Here, we describe a novel Gateway technology-compatible transposon plasmid that combines the advantages of Gateway recombination cloning with the Sleeping Beauty (SB) transposon-mediated transgene integrations. In our system the transposition is catalyzed by the novel hyperactive SB100x transposase, and provides highly efficient and precise transgene integrations into the host genome. A Gateway-compatible transposon plasmid was generated in which the potential target gene can be fused with a yellow fluorescent protein (YFP) tag at the N-terminal. The vector utilizes the CAGGS promoter to control fusion protein expression. The transposon expression vector encoding the YFP-interferon-β protein (IFNB1) fusion protein together with the hyperactive SB100x transposase was used to generate stable cell lines in human embryonic kidney (HEK293) and rat adipose-derived stromal cells (ASC). ASCs and HEK293 cells stably expressed and secreted the human IFNB1 for up to 4 weeks after transfection. The generated Gateway-compatible transposon plasmid can be utilized for numerous experimental approaches, such as gene therapy or high-throughput screening methods in primary cells, representing a valuable molecular tool for laboratory applications. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  16. Second-order derivatives of optical path length of ray with respect to variable vector of source ray. (United States)

    Chen, Yu-Bin; Lin, Psang Dain


    A method is proposed for determining the second-order derivatives (i.e., the Hessian matrix) of the optical path length of a ray with respect to the variable vector of the source ray in an optical system comprising both flat and spherical boundary surfaces. Several wavefront aberration problems are investigated using the Hessian matrix proposed in this study and the Jacobian (first-order derivatives) matrix presented in the literature. It is found that when using the Hessian matrix the precision of wavefront aberration is significantly improved when evaluated up to the quadratic term of the Taylor series expansion. The methodology proposed in this study not only provides the means to investigate the principal curvatures of the wavefront along a ray, but also yields the information required to determine the irradiance and caustics of both axisymmetric and nonaxisymmetric optical systems.

  17. Strain and vorticity patterns in ideally ductile transpression zones (United States)

    Robin, Pierre-Yves F.; Cruden, Alexander R.


    The prevalent model for ductile shear zones assumes that they develop by progressive simple shearing, resulting in a monoclinic fabric in which the vorticity vector is parallel to the shear zone and perpendicular to the lineation. But some ductile shear zones exhibit an amount of coaxial flattening, or a fabric pattern which appear to be incompatible with the assumptions of plane strain and progressive simple shear. In certain sections of the Archean Larder Lake—Cadillac deformation zone (LCDZ), for example, vorticity indicators (asymmetric pressure wings, Z-folds, SC fabrics), best seen on horizontal surfaces, indicate dextral transcurrent motion, whereas stretching lineations have variable but steep plunges. In the Proterozoic Mylonite Zone (MZ) of south-west Sweden, vorticity indicators combined with foliation and lineation data suggest a continuous change from reverse dip-slip motion close to the footwall to sinistral transcurrent motion adjacent to the hangingwall of the zone. Such departures from the ideal progressive simple shear zone pattern may in fact be common. Rather than invoke two stages of deformation, we explore the possibility that these patterns could be the result of ductile transpression. Ductile transpression between relatively rigid walls implies an extrusion of material out of the shear zone. When the material cannot slip freely along the boundaries of the zone, the extrusion strain is by necessity heterogeneous. In order to explore these heterogeneous strain distributions, we have developed a continuum mechanics model in which the 'transpressed' rock is a linear viscous material squeezed upward between two parallel, rigid, vertical walls. Transpression is further generalized by modelling oblique (i.e. with a dip-slip component) relative displacements of the walls. Models, which can vary in their obliquity and their 'press'/'trans' ratio, are examined for their distributions of K-values, strain rate intensity, 'lineation' (direction of

  18. Derivation of cloud-free-region atmospheric motion vectors from FY-2E thermal infrared imagery (United States)

    Wang, Zhenhui; Sui, Xinxiu; Zhang, Qing; Yang, Lu; Zhao, Hang; Tang, Min; Zhan, Yizhe; Zhang, Zhiguo


    The operational cloud-motion tracking technique fails to retrieve atmospheric motion vectors (AMVs) in areas lacking cloud; and while water vapor shown in water vapor imagery can be used, the heights assigned to the retrieved AMVs are mostly in the upper troposphere. As the noise-equivalent temperature difference (NEdT) performance of FY-2E split window (10.3-11.5 μm, 11.6-12.8 μm) channels has been improved, the weak signals representing the spatial texture of water vapor and aerosols in cloud-free areas can be strengthened with algorithms based on the difference principle, and applied in calculating AMVs in the lower troposphere. This paper is a preliminary summary for this purpose, in which the principles and algorithm schemes for the temporal difference, split window difference and second-order difference (SD) methods are introduced. Results from simulation and cases experiments are reported in order to verify and evaluate the methods, based on comparison among retrievals and the "truth". The results show that all three algorithms, though not perfect in some cases, generally work well. Moreover, the SD method appears to be the best in suppressing the surface temperature influence and clarifying the spatial texture of water vapor and aerosols. The accuracy with respect to NCEP 800 hPa reanalysis data was found to be acceptable, as compared with the accuracy of the cloud motion vectors.

  19. Atmospheric Vortices near Guadalupe Island (United States)


    These MISR images from June 11, 2000 (Terra orbit 2569) demonstrate a turbulent atmospheric flow pattern known as the von Karman vortex street. This phenomenon is named after aerodynamicist Theodore von Karman, who theoretically derived the conditions under which it occurs. The alternating double row of vortices can form in the wake of an obstacle, in this instance the eastern Pacific island of Guadalupe. The rugged terrain of this volcanic Mexican island reaches a maximum elevation of 1.3 kilometers. The island is about 35 kilometers long and is located 260 kilometers west of Baja California.The vortex pattern is made visible by the marine stratocumulus clouds around Guadalupe Island. The upper image is a color view obtained by MISR's vertical-viewing (nadir) camera. North is toward the left. The orientation of the vortex street indicates that the wind direction is from lower left to upper right (northwest to southeast). The areas within the vortex centers tend to be clear because the rotating motions induce a vertical wind component that can break up the cloud deck.The lower view is a stereo picture generated from data acquired by MISR's fore- and aft-viewing 70-degree cameras. A 3-D effect is obtained by viewing the image with red/blue glasses and placing the red filter over your left eye. Note how the downwelling atmospheric motion (change in elevation from high to low) is accompanied by a clearing in the center of the first vortex. As the vortices propagate downstream, their rotational velocities weaken. As a consequence, the induced vertical motion and cloud-clearing effect weakens as well.Theodore von Karman was a Professor of Aeronautics at Caltech and Director of Caltech's Guggenheim Aeronautical Laboratory from 1930-1949. He was one of the principal founders of the Jet Propulsion Laboratory.MISR was built and is managed by NASA's Jet Propulsion Laboratory, Pasadena, CA, for NASA's Office of Earth Science, Washington, DC. The Terra satellite is managed by

  20. Topological Interactions of Non-Abelian Vortices with Quasi-Particles in High Density QCD


    Hirono, Yuji; Kanazawa, Takuya; Nitta, Muneto


    Non-Abelian vortices are topologically stable objects in the color-flavor locked (CFL) phase of dense QCD. We derive a dual Lagrangian starting with the Ginzburg-Landau effective Lagrangian for the CFL phase, and obtain topological interactions of non-Abelian vortices with quasiparticles such as $U(1)_B$ Nambu-Goldstone bosons (phonons) and massive gluons. We find that the phonons couple to the translational zero modes of the vortices while the gluons couple to their orientational zero modes ...

  1. Jovian Upheaval and its Impact on Vortices (United States)

    de Pater, Imke


    We propose observations of Jupiter with global coverage at highresolution to quantify changes in its atmosphere during andfollowing the global upheaval. Only HST has the capability toobtain images with enough spatial resolution and contrast toextract velocity fields {we will use our newly developedtechnique to accomplish this}, and with WFC3 we can image Jupiterin its entirety in a single exposure. We are in particularinterested in the Red Oval BA: Will the Oval be long-lived,remain red, or turn white again, disappear? Both the merger ofits precursors, and change in color has never before beenwitnessed. The Great Red Spot: This storm system appears todecrease in size and has become rounder, both as derived from itsassociated cloud deck, but also from its potential vorticity, amore dynamically-relevant quantity. How will the GRS evolve? Willit swallow the new vortices detected in amateur images at thissame latitude band? How will this effect the potential vorticity?In addition, we hope to understand Disturbances and stagnationpoints, both of which were detected during the present globalupheaval: are these cyclonic regions, can they spawn anticyclones{as suggested by amateur images}?

  2. Algorithms for Computing the Magnetic Field, Vector Potential, and Field Derivatives for Circular Current Loops in Cylindrical Coordinates

    Energy Technology Data Exchange (ETDEWEB)

    Walstrom, Peter Lowell [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)


    A numerical algorithm for computing the field components Br and Bz and their r and z derivatives with open boundaries in cylindrical coordinates for circular current loops is described. An algorithm for computing the vector potential is also described. For the convenience of the reader, derivations of the final expressions from their defining integrals are given in detail, since their derivations (especially for the field derivatives) are not all easily found in textbooks. Numerical calculations are based on evaluation of complete elliptic integrals using the Bulirsch algorithm cel. Since cel can evaluate complete elliptic integrals of a fairly general type, in some cases the elliptic integrals can be evaluated without first reducing them to forms containing standard Legendre forms. The algorithms avoid the numerical difficulties that many of the textbook solutions have for points near the axis because of explicit factors of 1=r or 1=r2 in the some of the expressions.

  3. Algorithms for Computing the Magnetic Field, Vector Potential, and Field Derivatives for a Thin Solenoid with Uniform Current Density

    Energy Technology Data Exchange (ETDEWEB)

    Walstrom, Peter Lowell [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)


    A numerical algorithm for computing the field components Br and Bz and their r and z derivatives with open boundaries in cylindrical coordinates for radially thin solenoids with uniform current density is described in this note. An algorithm for computing the vector potential Aθ is also described. For the convenience of the reader, derivations of the final expressions from their defining integrals are given in detail, since their derivations are not all easily found in textbooks. Numerical calculations are based on evaluation of complete elliptic integrals using the Bulirsch algorithm cel. The (apparently) new feature of the algorithms described in this note applies to cases where the field point is outside of the bore of the solenoid and the field-point radius approaches the solenoid radius. Since the elliptic integrals of the third kind normally used in computing Bz and Aθ become infinite in this region of parameter space, fields for points with the axial coordinate z outside of the ends of the solenoid and near the solenoid radius are treated by use of elliptic integrals of the third kind of modified argument, derived by use of an addition theorem. Also, the algorithms also avoid the numerical difficulties the textbook solutions have for points near the axis arising from explicit factors of 1/r or 1/r2 in the some of the expressions.

  4. Lagrangian investigations of vorticity dynamics in compressible turbulence (United States)

    Parashar, Nishant; Sinha, Sawan Suman; Danish, Mohammad; Srinivasan, Balaji


    In this work, we investigate the influence of compressibility on vorticity-strain rate dynamics. Well-resolved direct numerical simulations of compressible homogeneous isotropic turbulence performed over a cubical domain of 10243 are employed for this study. To clearly identify the influence of compressibility on the time-dependent dynamics (rather than on the one-time flow field), we employ a well-validated Lagrangian particle tracker. The tracker is used to obtain time correlations between the instantaneous vorticity vector and the strain-rate eigenvector system of an appropriately chosen reference time. In this work, compressibility is parameterized in terms of both global (turbulent Mach number) and local parameters (normalized dilatation-rate and flow field topology). Our investigations reveal that the local dilatation rate significantly influences these statistics. In turn, this observed influence of the dilatation rate is predominantly associated with rotation dominated topologies (unstable-focus-compressing, stable-focus-stretching). We find that an enhanced dilatation rate (in both contracting and expanding fluid elements) significantly enhances the tendency of the vorticity vector to align with the largest eigenvector of the strain-rate. Further, in fluid particles where the vorticity vector is maximally misaligned (perpendicular) at the reference time, vorticity does show a substantial tendency to align with the intermediate eigenvector as well. The authors make an attempt to provide physical explanations of these observations (in terms of moment of inertia and angular momentum) by performing detailed calculations following tetrads {approach of Chertkov et al. ["Lagrangian tetrad dynamics and the phenomenology of turbulence," Phys. Fluids 11(8), 2394-2410 (1999)] and Xu et al. ["The pirouette effect in turbulent flows," Nat. Phys. 7(9), 709-712 (2011)]} in a compressible flow field.

  5. Far field velocity potential induced by a rapidly decaying vorticity distribution (United States)

    Klein, Rupert; Ting, LU


    The velocity field induced by a vorticity distribution decaying rapidly in the distance from the origin is investigated. It is shown that the sum of vector potentials for the velocity field can be expressed as a linear combination of Mn values, where Mn denotes the number of linearly independent vector functions of nth order. It is then shown that only the linear combinations 2n + 1 of these Mn vector functions contribute to the far field velocity which is irrotational, and that the corresponding scalar potential is then represented by a linear combination of 2n + 1 spherical harmonics of nth order whose coefficients are linear combinations of nth moments of vorticity.

  6. Vitality of optical vortices

    CSIR Research Space (South Africa)

    Roux, FS


    Full Text Available (t) = ∂tv(t) = ∂tx(t)xˆ + ∂ty(t)yˆ + ∂tz(t)zˆ. (9) where ∂t = ∂/∂t. The sign of the z-component of the acceleration vector tells us whether the vortex line curves forward or backward (see Fig. 1). It can therefore be used to distinguish between creation...

  7. Efficient and sustained IGF-1 expression in the adipose tissue-derived stem cells mediated via a lentiviral vector. (United States)

    Chen, Ting; Huang, Dangsheng; Chen, Guanghui; Yang, Tingshu; Yi, Jun; Tian, Miao


    The adipose tissue-derived stem cells (ADSCs) represent a significant area of the cell therapy. Genetic modification of ADSCs may further improve their therapeutic potential. Here, we aimed to generate a lentiviral vector expressing insulin-like growth factor-I (IGF-1) and investigate the impact of IGF-1 transduction on the properties of cultured ADSCs. Isolated rat ADSCs were assessed by flow cytometric analysis. IGF-1 was cloned and inserted into the pLenO-DCE plasmid to acquire pLenO-DCE-IGF-1 plasmid. Lentivirus was enveloped with pRsv-REV, pMDlg-pRRE and pMD2G plasmids in 293T cells. The ADSCs were transfected with the vectors. And then IGF-1-induced anti-apoptosis was evaluated by annexin V-FITC. Besides, proliferation of cells was detected by MTT assay and EdU. Moreover, Akt phosphorylation was evaluated by Western blotting analysis. Stable expression of IGF-1 in ADSCs was confirmed. ADSCs were positive for CD90 and CD29, but negative for CD31, CD34 and CD45. The transduction of IGF-1 to the ADSCs caused a dramatic increase in P-Akt expression. Over-expression of IGF-1 in ADSCs could improve the paracine of IGF-1 in a time-dependent manner, but could not promote the proliferation of ADSCs. This study indicated that lentiviral vectors offered a promising mean of delivering IGF-1 to the ADSCs. Lentiviral-mediated over-expression of therapeutic IGF-1 gene in ADSCs could prolong the anti-apoptosis effect of IGF-1, which might be induced by the activation of the PI3K/Akt pathway. And our data would improve the efficacy of ADSC-based therapies.

  8. Baroclinic Vortices in Rotating Stratified Shearing Flows: Cyclones, Anticyclones, and Zombie Vortices (United States)

    Hassanzadeh, Pedram

    Large coherent vortices are abundant in geophysical and astrophysical flows. They play significant roles in the Earth's oceans and atmosphere, the atmosphere of gas giants, such as Jupiter, and the protoplanetary disks around forming stars. These vortices are essentially three-dimensional (3D) and baroclinic, and their dynamics are strongly influenced by the rotation and density stratification of their environments. This work focuses on improving our understanding of the physics of 3D baroclinic vortices in rotating and continuously stratified flows using 3D spectral simulations of the Boussinesq equations, as well as simplified mathematical models. The first chapter discusses the big picture and summarizes the results of this work. In Chapter 2, we derive a relationship for the aspect ratio (i.e., vertical half-thickness over horizontal length scale) of steady and slowly-evolving baroclinic vortices in rotating stratified fluids. We show that the aspect ratio is a function of the Brunt-Vaisala frequencies within the vortex and outside the vortex, the Coriolis parameter, and the Rossby number of the vortex. This equation is basically the gradient-wind equation integrated over the vortex, and is significantly different from the previously proposed scaling laws that find the aspect ratio to be only a function of the properties of the background flow, and independent of the dynamics of the vortex. Our relation is valid for cyclones and anticyclones in either the cyclostrophic or geostrophic regimes; it works with vortices in Boussinesq fluids or ideal gases, and non-uniform background density gradient. The relation for the aspect ratio has many consequences for quasi-equilibrium vortices in rotating stratified flows. For example, cyclones must have interiors more stratified than the background flow (i.e., super-stratified), and weak anticyclones must have interiors less stratified than the background (i.e., sub-stratified). In addition, this equation is useful to

  9. Vorticity scaling and intermittency in drift-interchange plasma turbulence

    Energy Technology Data Exchange (ETDEWEB)

    Dura, P. D.; Hnat, B.; Robinson, J.; Dendy, R. O. [Centre for Fusion, Space and Astrophysics, Department of Physics, University of Warwick, Coventry CV4 7AL (United Kingdom)


    The effects of spatially varying magnetic field strength on the scaling properties of plasma turbulence, modelled by an extended form of Hasegawa-Wakatani model, are investigated. We study changes in the intermittency of the velocity, density, and vorticity fields, as functions of the magnetic field inhomogeneity C=-{partial_derivative} ln B/{partial_derivative}x. While the velocity fluctuations are always self-similar and their scaling is unaffected by the value of C, the intermittency levels in density and vorticity change with parameter C, reflecting morphological changes in the coherent structures due to the interchange mechanism. Given the centrality of vorticity in conditioning plasma transport, this result is of interest in scaling the results of transport measurements and simulations in tokamak edge plasmas, where drift-interchange turbulence in the presence of a magnetic field gradient is likely to occur.

  10. Cubic nonlinear Schroedinger equation with vorticity

    Energy Technology Data Exchange (ETDEWEB)

    Caliari, M; Morato, L M; Zuccher, S [Dipartimento di Informatica, Universita di Verona, Ca' Vignal 2, Strada Le Grazie 15, 37134 Verona (Italy); Loffredo, M I [Dipartimento di Scienze Matematiche ed Informatiche, Universita di Siena, Pian dei Mantellini 44, 53100 Siena (Italy)], E-mail:, E-mail:, E-mail:, E-mail:


    In this paper, we introduce a new class of nonlinear Schroedinger equations (NLSEs), with an electromagnetic potential (A,{phi}), both depending on the wavefunction {psi}. The scalar potential {phi} depends on |{psi}|{sup 2}, whereas the vector potential A satisfies the equation of magnetohydrodynamics with coefficient depending on {psi}. In Madelung variables, the velocity field comes to be not irrotational in general and we prove that the vorticity induces dissipation, until the dynamical equilibrium is reached. The expression of the rate of dissipation is common to all NLSEs in the class. We show that they are a particular case of the one-particle dynamics out of dynamical equilibrium for a system of N identical interacting Bose particles, as recently described within stochastic quantization by Lagrangian variational principle. The cubic case is discussed in particular. Results of numerical experiments for rotational excitations of the ground state in a finite two-dimensional trap with harmonic potential are reported.

  11. The use of Full Earth Rotation Vector Derived from Ring Laser Data (United States)

    Hugentobler, Urs; Schreiber, Ulrich; Panafidina, Natascha; Gebauer, André


    Current active ring laser technology demonstrates the capability to measure variations of the rotation of the Earth. The large ring laser gyroscope "G" at the Geodetic Observatory Wettzell, Germany, shows a stability which allows the measurement of the wobble of the Earth axis. Earth rotation currently is determined routinely in the framework of the International Earth Rotation and Reference Systems Service (IERS) using space geodetic techniques such as Global Navigation Satellite Systems (GNSS), Satellite Laser Ranging (SLR), and Very Long Baseline Interferometry (VLBI). These techniques measure the rotation of the Earth kinematically by determining the motion of ground stations with respect to space objects, i.e., based on a technique also called "stellar compass". Ring laser gyroscopes on the other hand measure Earth rotation locally based on the observation of inertial accelerations, i.e., based on a technique also called "inertial compass". The fundamentally different measurement principles complement each other. It is, e.g., well known that forced polar motion, the so called Oppolzer terms, are not observable by space geodetic techniques due to strong correlation with the motion of the Earth axis in space. For the same reason the determination of subdaily Earth rotation parameters with space geodetic techniques is prone to strong systematic errors while exact knowledge of such terms is relevant, e.g., to determine high quality orbits of GNSS satellites. This presentation shall highlight the potential of the ring laser technology as additional sensor for monitoring Earth rotation, in particular with the upcoming capability to measure the full Earth rotation vector.

  12. Adeno-associated virus vector-mediated delivery of pigment epithelium-derived factor restricts neuroblastoma angiogenesis and growth. (United States)

    Streck, Christian J; Zhang, Youbin; Zhou, Junfang; Ng, Catherine; Nathwani, Amit C; Davidoff, Andrew M


    The purpose of this study was to evaluate the ability of adeno-associated virus (AAV) vector-mediated delivery of pigment epithelium-derived factor (PEDF) to inhibit neuroblastoma (NB) xenograft growth. Pigment epithelium-derived factor was chosen for this study because, in addition to being a potent inhibitor of angiogenesis, it is capable of inducing neuronal differentiation. Cohorts of mice received either recombinant AAV encoding human PEDF (rAAV-hPEDF) at a range of doses or control vector via tail vein. Subsequent hPEDF expression was measured by enzyme-linked immunoassay. After 6 weeks, the mice were given human NB cells by retroperitoneal injection and then killed 5 weeks later. Tumor weight, microvessel density, tumor differentiation, apoptosis, and levels of intratumoral vascular endothelial growth factor (VEGF) expression were determined at that time. In subsequent cohorts of mice, AAV-mediated murine PEDF expression was tested against both human NB xenografts and murine tumors. In a series of in vitro studies, PEDF was shown to inhibit endothelial cell activation and to stimulate differentiation of NB cell lines. After tail vein injection of rAAV-hPEDF, stable transgene expression was generated and correlated with levels of vector administration. Human NB xenograft growth was restricted by hPEDF in a dose-dependent fashion. Intratumoral VEGF expression and microvessel density were decreased, and tumor cell apoptosis was increased in PEDF-treated mice. Treatment with PEDF had a significant impact on NB growth in mice when delivered continuously using a gene therapy-mediated approach. The activity of PEDF appears to be mediated in part by inhibition of tumor-induced angiogenesis through down-regulation of tumor-elaborated VEGF, with subsequent intratumoral apoptosis. Furthermore, hPEDF was able to induce NB differentiation in vitro and in vivo. In addition, antitumor efficacy was seen when mouse PEDF was used to treat syngeneic murine tumors. In our

  13. Active Control of Stationary Vortices (United States)

    Nino, Giovanni; Breidenthal, Robert; Bhide, Aditi; Sridhar, Aditya


    A system for active stationary vortex control is presented. The system uses a combination of plasma actuators, pressure sensors and electrical circuits deposited on aerodynamic surfaces using printing electronics methods. Once the pressure sensors sense a change on the intensity or on the position of the stationary vortices, its associated controller activates a set of plasma actuator to return the vortices to their original or intended positions. The forces produced by the actuators act on the secondary flow in the transverse plane, where velocities are much less than in the streamwise direction. As a demonstration case, the active vortex control system is mounted on a flat plate under low speed wind tunnel testing. Here, a set of vortex generators are used to generate the stationary vortices and the plasma actuators are used to move them. Preliminary results from the experiments are presented and compared with theoretical values. Thanks to the USAF AFOSR STTR support under contract # FA9550-15-C-0007.

  14. Barriers for Deriving Transgene-Free Pig iPS Cells with Episomal Vectors. (United States)

    Du, Xuguang; Feng, Tao; Yu, Dawei; Wu, Yuanyuan; Zou, Huiying; Ma, Shuangyu; Feng, Chong; Huang, Yongye; Ouyang, Hongsheng; Hu, Xiaoxiang; Pan, Dengke; Li, Ning; Wu, Sen


    To date no authentic embryonic stem cell (ESC) line or germline-competent-induced pluripotent stem cell (iPSC) line has been established for large animals. Despite this fact, there is an impression in the field that large animal ESCs or iPSCs are as good as mouse counterparts. Clarification of this issue is important for a healthy advancement of the stem cell field. Elucidation of the causes of this failure in obtaining high quality iPSCs/ESCs may offer essential clues for eventual establishment of authentic ESCs for large animals including humans. To this end, we first generated porcine iPSCs using nonintegrating replicating episomal plasmids. Although these porcine iPSCs met most pluripotency criteria, they could neither generate cloned piglets through nuclear transfer, nor contribute to later stage chimeras through morula injections or aggregations. We found that the reprogramming genes in iPSCs could not be removed even under negative selection, indicating they are required to maintain self-renewal. The persistent expression of these genes in porcine iPSCs in turn caused differentiation defects in vivo. Therefore, incomplete reprogramming manifested by a reliance on sustained expression of exogenous-reprogramming factors appears to be the main reason for the inability of porcine iPSCs to form iPSC-derived piglets. © 2015 AlphaMed Press.

  15. A generalised form of Lait's modified potential vorticity (United States)

    Mueller, R.; Guenther, G.


    Ertel's potential vorticity P is in wide spread use as a diagnostic of dynamical processes in the stratosphere. For a variety of applications however, the exponential increase of P with altitude is problematic. For this reason, Lait, (JAS, 1994) proposed a modified potential vorticity Pi_L, where a physically meaningful scaling is introduced that removes much of the altitude dependence of P. % Here we propose a generalised form of Pi_L by introducing an additional degree of freedom in the scaling. Such a scaling may alternatively be derived by considering a more general form of potential vorticity, where polytropic temperature rather than potential temperature is used as a hydrodynamical invariant. This generalised form of Pi_L possesses the same conservation properties as Pi_L itself and as the classical potential vorticity P , but can be adjusted more closely to the specific situation under investigation. Comparison, over a large altitude range in the stratosphere, of fields of the generalised modified potential vorticity Pi_g with dynamical measures of the polar vortex edge and with observations of the long-lived trace gas N_2O show that Pi_g constitutes a more intuitively interpretable quantity than Pi_L.

  16. Unstable vortices do not confine

    NARCIS (Netherlands)

    Achúcarro, A.; Roo, M. de; Huiszoon, L.; Landshoff, P.V.


    Recently, a geometric model for the confinement of magnetic charges in the context of type II string compactifications was constructed. This model assumes the existence of stable magnetic vortices with quantized flux in the low energy theory. However, quantization of flux alone does not imply that

  17. Frame dragging, vorticity and electromagnetic fields in axially symmetric stationary spacetimes

    Energy Technology Data Exchange (ETDEWEB)

    Herrera, L [Escuela de Fisica, Universidad Central de Venezuela, Caracas, Venezuela (Venezuela); Gonzalez, G A [Escuela de Fisica, Universidad Industrial de Santander, AA 678, Bucaramanga (Colombia); Pachon, L A [Escuela de Fisica, Universidad Industrial de Santander, AA 678, Bucaramanga (Colombia); Laboratorio de AstronomIa y Fisica Teorica (LAFT), Departamento de Fisica, Facultad de Ciencias, La Universidad del Zulia, Maracaibo, 4004 (Venezuela); Rueda, J A [Escuela de Fisica, Universidad Industrial de Santander, AA 678, Bucaramanga (Colombia)


    We present a general study about the relation between the vorticity tensor and the Poynting vector of the electromagnetic field for axially symmetric stationary electrovacuum metrics. The obtained expressions allow us to understand the role of the Poynting vector in the dragging of inertial frames. The particular case of the rotating massive charged magnetic dipole is analysed in detail. In addition, the electric and magnetic parts of the Weyl tensor are calculated and the link between the latter and the vorticity is established. Then we show that, in the vacuum case, the necessary and sufficient condition for the vanishing of the magnetic part is that the spacetime be static.

  18. Review of vortices in wildland fire (United States)

    Jason M. Forthofer; Scott L. Goodrick


    Vortices are almost always present in the wildland fire environment and can sometimes interact with the fire in unpredictable ways, causing extreme fire behavior and safety concerns. In this paper, the current state of knowledge of the interaction of wildland fire and vortices is examined and reviewed. A basic introduction to vorticity is given, and the two common...

  19. Phenotyping of VIGS-mediated gene silencing in rice using a vector derived from a DNA virus. (United States)

    Kant, Ravi; Dasgupta, Indranil


    Target genes in rice can be optimally silenced if inserted in antisense or hairpin orientation in the RTBV-derived VIGS vector and plants grown at 28 °C and 80% humidity after inoculation. Virus induced gene silencing (VIGS) is a method used to transiently silence genes in dicot as well as monocot plants. For the important monocot species rice, the Rice tungro bacilliform virus (RTBV)-derived VIGS system (RTBV-VIGS), which uses agroinoculation to initiate silencing, has not been standardized for optimal use. Here, using RTBV-VIGS, three sets of conditions were tested to achieve optimal silencing of the rice marker gene phytoene desaturase (pds). The effect of orientation of the insert in the RTBV-VIGS plasmid (sense, antisense and hairpin) on the silencing of the target gene was then evaluated using rice magnesium chelatase subunit H (chlH). Finally, the rice Xa21 gene, conferring resistance against bacterial leaf blight disease (BLB) was silenced using RTBV-VIGS system. In each case, real-time PCR-based assessment indicated approximately 40-80% fall in the accumulation levels of the transcripts of pds, chlH and Xa21. In the case of pds, the appearance of white streaks in the emerging leaves, and for chlH, chlorophyll levels and F v /F m ratio were assessed as phenotypes for silencing. For Xa21, the resistance levels to BLB were assessed by measuring the lesion length and the percent diseased areas of leaves, following challenge inoculation with Xanthomonas oryzae. In each case, the RTBV-MVIGS system gave rise to a discernible phenotype indicating the silencing of the respective target gene using condition III (temperature 28 °C, humidity 80% and 1 mM MES and 20 µM acetosyringone in secondary agrobacterium culture), which revealed the robustness of this gene silencing system for rice.

  20. The life-cycle of Riemann-Silberstein electromagnetic vortices (United States)

    Nye, J. F.


    To study the singularities of a monochromatic electromagnetic wave field in free space, it is desirable to use a quantity that combines both the electric field E and the magnetic field B in equal measure. The Riemann-Silberstein (R-S) field is a way of doing this. It is based on the real physical E and B and one constructs from them the complex vector field {F}={E}+{{i}} {B}. Then, one constructs {F}\\cdot {F} and studies the optical vortices of this R-S complex scalar field. Unlike the better-known and much studied optical vortices of a monochromatic complex scalar field, which are stationary, these vortices are normally in continual motion; they oscillate at the optical frequency. We study their life cycle in the simplest model that is sufficiently generic, namely, fields generated by the interference of four randomly chosen plane elliptically polarised waves. The topological events in the life cycle do not repeat on a 3D space lattice in a stationary laboratory frame. In space-time, however, the R-S vortices are invariant under any Lorentz transformation, and because of this and the inherent time repetition there is a particular moving frame in space-time, reached by a Lorentz transformation, where there exists a repeating pattern of events in space. Its 4D unit cell constitutes, in effect, a description of the whole infinite pattern. Just because they are in constant motion, it is not surprising that the R-S vortex lines in the model make reconnections and appear as rings that either shrink to nothing or appear from nothing. However, these processes occur in groups of four, reflecting the fact that the unit cell is face-centred. What distinguishes the R-S field from the other complex scalar fields containing vortices is the existence of this face-centred repeating cell.

  1. Vortical flows in technical applications


    Krause, Egon


    Two examples of flows dominated by vortical structures are discussed: In the first interaction and decay of vortex structures in in-cylinder flows of automotive engines are described. Numerical studies revealed clearly identifiable vortex rings, generated during the intake stroke. The influence of compressibility on the vortex formation was studied by using Mach-Zehnder interferometry in a specially designed test stand of a towed one-cylinder engine, and with numerical solutions of the Navier...

  2. Vortices in polariton OPO superfluids


    Marchetti, F. M.; Szymanska, M. H.


    his chapter reviews the occurrence of quantised vortices in polariton fluids, primarily when polaritons are driven in the optical parametric oscillator (OPO) regime. We first review the OPO physics, together with both its analytical and numerical modelling, the latter being necessary for the description of finite size systems. Pattern formation is typical in systems driven away from equilibrium. Similarly, we find that uniform OPO solutions can be unstable to the spontaneous formation of quan...

  3. Buoyancy-Induced, Columnar Vortices (United States)

    Simpson, Mark; Glezer, Ari


    Free buoyancy-induced, columnar vortices (dust devils) that are driven by thermal instabilities of ground-heated, stratified air in areas with sufficient insolation convert the potential energy of low-grade heat in the surface air layer into a vortex flow with significant kinetic energy. A variant of the naturally-occurring vortex is deliberately triggered and anchored within an azimuthal array of vertical, stator-like flow vanes that form an open-top enclosure and impart tangential momentum to the radially entrained air. This flow may be exploited for power generation by coupling the vortex to a vertical-axis turbine. The fundamental mechanisms associated with the formation, evolution, and dynamics of an anchored, buoyancy-driven columnar vortex within such a facility are investigated experimentally using a heated ground plane. Specific emphasis is placed on the manipulation of the vortex formation and structure and the dependence of the vorticity production and sustainment mechanisms on the thermal resources and characteristic scales of the anchoring flow vanes using stereo-PIV. It is shown that manipulation of the formation and advection of vorticity concentrations within the enclosure can be exploited for increasing the available kinetic energy. Supported by ARPA-E.

  4. pMAA-Red: a new pPZP-derived vector for fast visual screening of transgenic Arabidopsis plants at the seed stage

    Directory of Open Access Journals (Sweden)

    Ali Muhammad


    Full Text Available Abstract Background The production of transgenic plants, either for the overproduction of the protein of interest, for promoter: reporter lines, or for the downregulation of genes is an important prerequisite in modern plant research but is also very time-consuming. Results We have produced additions to the pPZP family of vectors. Vector pPZP500 (derived from pPZP200 is devoid of NotI sites and vector pPZP600 (derived from pPZP500 contains a bacterial kanamycin resistance gene. Vector pMAA-Red contains a Pdf2.1: DsRed marker and a CaMV:: GUS cassette within the T-DNA and is useful for the production of promoter: GUS lines and overexpression lines. The Pdf2.1 promoter is expressed in seeds and syncytia induced by the beet cyst nematode Heterodera schachti in Arabidopsis roots. Transgenic seeds show red fluorescence which can be used for selection and the fluorescence level is indicative of the expression level of the transgene. The advantage is that plants can be grown on soil and that expression of the marker can be directly screened at the seed stage which saves time and resources. Due to the expression of the Pdf2.1: DsRed marker in syncytia, the vector is especially useful for the expression of a gene of interest in syncytia. Conclusions The vector pMAA-Red allows for fast and easy production of transgenic Arabidopsis plants with a strong expression level of the gene of interest.

  5. Measurements of Vorticity Vectors in Couette Flow with the Vorticity Optical Probe (United States)


    pigmentation additive ZTX-B (formerly a product of the Mearl Corporation, now available on foreign suppliers). containing basic lead carbonate mirrors was...most fruitful avenue employed acrylamide gel. This water soluble polymer network effectively turns the PMMA model inside-out, with a non-aqueous buffer...Hesselink. L. t 1990L "Application of Holography to the Measurement of Velocity Gradients in Fluid Flows Sensitized with Photochromic Dyes, Phys. Fluids A

  6. Topology of streamlines and vorticity contours for two - dimensional flows

    DEFF Research Database (Denmark)

    Andersen, Morten

    Considering a coordinate-free formulation of helical symmetry rather than more traditional definitions based on coordinates, we discuss basic properties of helical vector fields and compare results from the literature. For inviscid flow where a velocity field is generated by a sum of helical vortex...... the singularities are treated as centers. The last topology is a single center which is consistent with the powerful result on the long time behaviour proved by Gallay & Wayne (Comm. in Math. Phys. 255, 2005). The case of three critical points of the streamlines is a subset of three critical points of the vorticity...

  7. First measurements of electron vorticity in the foreshock and solar wind

    Energy Technology Data Exchange (ETDEWEB)

    Gurgiolo, C. [Bitterroot Basic Research, Hamilton, MT (United States); Goldstein, M.L.; Vinas, A.F. [NASA Goddard Space Flight Center, Greenbelt, MD (United States). Geospace Science Lab.; Fazakerley, A.N. [University College London (United Kingdom). Mullard Space Science Lab.


    We describe the methodology used to set up and compute spatial derivatives of the electron moments using data acquired by the Plasma Electron And Current Experiment (PEACE) from the four Cluster spacecraft. The results are used to investigate electron vorticity in the foreshock. We find that much of the measured vorticity, under nominal conditions, appears to be caused by changes in the flow direction of the return (either reflected or leakage from the magnetosheath) and strahl electron populations as they couple to changes in the magnetic field orientation. This in turn results in deflections in the total bulk velocity producing the measured vorticity. (orig.)

  8. Making sound vortices by metasurfaces

    CERN Document Server

    Ye, Liping; Lu, Jiuyang; Tang, Kun; Jia, Han; Ke, Manzhu; Peng, Shasha; Liu, Zhengyou


    Based on the Huygens-Fresnel principle, a metasurface structure is designed to generate a sound vortex beam in airborne environment. The metasurface is constructed by a thin planar plate perforated with a circular array of deep subwavelength resonators with desired phase and amplitude responses. The metasurface approach in making sound vortices is validated well by full-wave simulations and experimental measurements. Potential applications of such artificial spiral beams can be anticipated, as exemplified experimentally by the torque effect exerting on an absorbing disk.

  9. Making sound vortices by metasurfaces

    Energy Technology Data Exchange (ETDEWEB)

    Ye, Liping; Qiu, Chunyin, E-mail:; Lu, Jiuyang; Tang, Kun; Ke, Manzhu; Peng, Shasha [Key Laboratory of Artificial Micro- and Nano-structures of Ministry of Education and School of Physics and Technology, Wuhan University, Wuhan 430072 (China); Jia, Han [State Key Laboratory of Acoustics and Key Laboratory of Noise and Vibration Research, Institute of Acoustics, Chinese Academy of Sciences, Beijing 100190 (China); Liu, Zhengyou [Key Laboratory of Artificial Micro- and Nano-structures of Ministry of Education and School of Physics and Technology, Wuhan University, Wuhan 430072 (China); Institute for Advanced Studies, Wuhan University, Wuhan 430072 (China)


    Based on the Huygens-Fresnel principle, a metasurface structure is designed to generate a sound vortex beam in airborne environment. The metasurface is constructed by a thin planar plate perforated with a circular array of deep subwavelength resonators with desired phase and amplitude responses. The metasurface approach in making sound vortices is validated well by full-wave simulations and experimental measurements. Potential applications of such artificial spiral beams can be anticipated, as exemplified experimentally by the torque effect exerting on an absorbing disk.

  10. An application of remotely derived climatological fields for risk assessment of vector-borne diseases : a spatial study of filariasis prevalence in the Nile Delta, Egypt.

    Energy Technology Data Exchange (ETDEWEB)

    Crombie, M. K.; Gillies, R. R.; Arvidson, R. E.; Brookmeyer, P.; Weil, G. J.; Sultan, M.; Harb, M.; Environmental Research; Washington Univ.; Utah State Univ.; Egyptian Ministry of Health


    This paper applies a relatively straightforward remote sensing method that is commonly used to derive climatological variables. Measurements of surface reflectance and surface radiant temperature derived from Landsat Thematic Mapper data were used to create maps of fractional vegetation and surface soil moisture availability for the southern Nile delta in Egypt. These climatological variables were subsequently used to investigate the spatial distribution of the vector borne disease Bancroftian filariasis in the Nile delta where it is focally endemic and a growing problem. Averaged surface soil moisture values, computed for a 5-km border area around affected villages, were compared to filariasis prevalence rates. Prevalence rates were found to be negligible below a critical soil moisture value of 0.2, presumably because of a lack of appropriate breeding sites for the Culex Pipiens mosquito species. With appropriate modifications to account for local conditions and vector species, this approach should be useful as a means to map, predict, and control insect vector-borne diseases that critically depend on wet areas for propagation. This type of analysis may help governments and health agencies that are involved in filariasis control to better focus limited resources to identifiable high-risk areas.

  11. Observations of Electron Vorticity in the Inner Plasmasheet and Its Relationship to Reconnection (United States)

    Gurgiolo, Chris A.; Goldstein, Melvyn L.; Matthaeus, William H.; Vinas, Adolfo -F.


    Spatial derivatives of the electron moments can be estimated using data from the four Cluster spacecraft. Using spatial derivatives of the velocity we have computed the vorticity in the plasmasheet for several crossings. What we have found is that vorticity appears to be a common feature in the inner plasmasheet. We will show a number of examples. In at least some of the observations the vorticity is well correlated with the passage of Cluster through the ion diffusion region of known reconnection events. That most of the vorticity events observed are reconnection related cannot be dismissed and in fact observations of vorticity may provide a means to locate times when the Cluster spacecraft are magnetically connected to regions where reconnection is taking place. Understanding the role and source of the vorticity should advance our understanding of the dissipation of the turbulence associated with reconnection. In the course of the presentation we will also touch on the methods used to estimate the spatial derivatives as well as the limitations and assumptions involved.

  12. Adeno-associated viral vector-mediated gene transfer of brain-derived neurotrophic factor reverses atrophy of rubrospinal neurons following both acute and chronic spinal cord injury. (United States)

    Ruitenberg, Marc J; Blits, Bas; Dijkhuizen, Paul A; te Beek, Erik T; Bakker, Arne; van Heerikhuize, Joop J; Pool, Chris W; Hermens, Wim T J; Boer, Gerard J; Verhaagen, Joost


    Rubrospinal neurons (RSNs) undergo marked atrophy after cervical axotomy. This progressive atrophy may impair the regenerative capacity of RSNs in response to repair strategies that are targeted to promote rubrospinal tract regeneration. Here, we investigated whether we could achieve long-term rescue of RSNs from lesion-induced atrophy by adeno-associated viral (AAV) vector-mediated gene transfer of brain-derived neurotrophic factor (BDNF). We show for the first time that AAV vectors can be used for the persistent transduction of highly atrophic neurons in the red nucleus (RN) for up to 18 months after injury. Furthermore, BDNF gene transfer into the RN following spinal axotomy resulted in counteraction of atrophy in both the acute and chronic stage after injury. These novel findings demonstrate that a gene therapeutic approach can be used to reverse atrophy of lesioned CNS neurons for an extended period of time.

  13. Murine leukemia virus-derived retroviral vector has differential integration patterns in human cell lines used to produce recombinant factor VIII

    Directory of Open Access Journals (Sweden)

    Marcela Cristina Correa de Freitas


    Full Text Available OBJECTIVE: Nowadays recombinant factor VIII is produced in murine cells including in Chinese hamster ovary (CHO and baby hamster kidney cells (BHK. Previous studies, using the murine leukemia virus-derived retroviral vector pMFG-FVIII-P140K, modified two recombinant human cell lines, HepG2 and Hek293 to produce recombinant factor VIII. In order to characterize these cells, the present study aimed to analyze the integration pattern of retroviral vector pMFG-FVIII-P140K.METHODS: This study used ligation-mediated polymerase chain reaction to locate the site of viral vector integration by sequencing polymerase chain reaction products. The sequences were compared to genomic databases to characterize respective clones.RESULTS: The retroviral vector presented different and non-random profiles of integration between cells lines. A preference of integration for chromosomes 19, 17 and 11 was observed for HepG2FVIIIdB/P140K and chromosome 9 for Hek293FVIIIdB/P140K. In genomic regions such as CpG islands and transcription factor binding sites, there was no difference in the integration profiles for both cell lines. Integration in intronic regions of encoding protein genes (RefSeq genes was also observed in both cell lines. Twenty percent of integrations occurred at fragile sites in the genome of the HepG2 cell line and 17% in Hek293.CONCLUSION: The results suggest that the cell type can affect the profile of chromosomal integration of the retroviral vector used; these differences may interfere in the level of expression of recombinant proteins.

  14. On generating counter-rotating streamwise vortices

    KAUST Repository

    Winoto, S H


    Counter-rotating streamwise vortices are known to enhance the heat transfer rate from a surface and also to improve the aerodynamic performance of an aerofoil. In this paper, some methods to generate such counter-rotating vortices using different methods or physical conditions will be briefly considered and discussed.


    Yamasaki, T.; Kawaji, H.; Kamio, Y.; Amano, S.; Sameshima, T.; Sakai, N.; Tokuyama, T.; Namba, H.


    Suicide gene therapy using genetically engineered stem cells are is one of the most feasible and promising approaches for glioma therapy. Various stem cells, such as neural and mesenchymal stem cells, have been tested for their tumor tropic activity. We have tested stem cells transduced with the herpes simplex virus-thymidine kinase gene (HSV-TK, suicide gene) encoding a viral thymidine kinase which phosphorylates prodrug ganciclovir (GCV) for experimental glioma in rodents, because the HSV-TK/GCV system generates a potent bystander effect. Recently we found that induced pluripotent stem cells (iPSs) also had tumor tropic activity under both in vitro and in vivo conditions. One of the major limitations of use of HSV-TK gene-trasnduced iPSs in clinical field is the use of virus vectors that randomly integrate into the host genome and are associated with the risk of malignant transformation due to insertional mutagenesis. In the present study, we present a non-viral transfection method for obtaining HSV-TK expressing iPS-derived neural stem cells to circumvent the concerns associated with use of viral vectors. Mouse iPS cells were generated from somatic cells by the plasmid vectors expressing Oct4, Sox2, Klf4, c-Myc and Nanog-GFP-IRES-Puror. We differentiated the mouse iPS cells into neural stem cells and then transfected with the plasmid containing HSV-TK gene using electroporation method. In this way, we obtained HSV-TK gene-trasnduced iPSs-derived neural stem cells without using viral vectors for further pre-clinical expariments of glioma therapy.

  16. Theory of concentrated vortices an introduction

    CERN Document Server

    Alekseenko, S V; Okulov, V L


    Vortex motion is one of the basic states of a flowing continuum. Intere- ingly, in many cases vorticity is space-localized, generating concentrated vortices. Vortex filaments having extremely diverse dynamics are the most characteristic examples of such vortices. Notable examples, in particular, include such phenomena as self-inducted motion, various instabilities, wave generation, and vortex breakdown. These effects are typically ma- fested as a spiral (or helical) configuration of a vortex axis. Many publications in the field of hydrodynamics are focused on vortex motion and vortex effects. Only a few books are devoted entirely to v- tices, and even fewer to concentrated vortices. This work aims to highlight the key problems of vortex formation and behavior. The experimental - servations of the authors, the impressive visualizations of concentrated vortices (including helical and spiral) and pictures of vortex breakdown primarily motivated the authors to begin this work. Later, the approach based on the hel...

  17. Magnetoplasmonic control of plasmonic vortices (United States)

    Maccaferri, Nicolò; Gorodetski, Yuri; Toma, Andrea; Zilio, Pierfrancesco; De Angelis, Francesco; Garoli, Denis


    We theoretically investigate the generation of far-field propagating optical beams with a desired orbital angular momentum by using an archetypical magnetoplasmonic tip surrounded by a gold spiral slit. The use of a magnetic material can lead to important implications once magneto-optical activity is activated through the application of an external magnetic field. The physical model and the numerical study presented here introduce the concept of magnetically tunable plasmonic vortex lens, namely a magnetoplasmonic vortex lens, which ensures a tunable selectivity in the polarization state of the generated nanostructured beam. The presented system provides a promising platform for a localized excitation of plasmonic vortices followed by their beaming in the far-field with an active modulation of both light's transmission and helicity.

  18. Characterization of rice black-streaked dwarf virus- and rice stripe virus-derived siRNAs in singly and doubly infected insect vector Laodelphax striatellus. (United States)

    Li, Junmin; Andika, Ida Bagus; Shen, Jiangfeng; Lv, Yuanda; Ji, Yongqiang; Sun, Liying; Chen, Jianping


    Replication of RNA viruses in insect cells triggers an antiviral defense that is mediated by RNA interference (RNAi) which generates viral-derived small interfering RNAs (siRNAs). However, it is not known whether an antiviral RNAi response is also induced in insects by reoviruses, whose double-stranded RNA genome replication is thought to occur within core particles. Deep sequencing of small RNAs showed that when the small brown planthopper (Laodelphax striatellus) was infected by Rice black-streaked dwarf virus (RBSDV) (Reoviridae; Fijivirus), more viral-derived siRNAs accumulated than when the vector insect was infected by Rice stripe virus (RSV), a negative single-stranded RNA virus. RBSDV siRNAs were predominantly 21 and 22 nucleotides long and there were almost equal numbers of positive and negative sense. RBSDV siRNAs were frequently generated from hotspots in the 5'- and 3'-terminal regions of viral genome segments but these hotspots were not associated with any predicted RNA secondary structures. Under laboratory condition, L. striatellus can be infected simultaneously with RBSDV and RSV. Double infection enhanced the accumulation of particular genome segments but not viral coat protein of RBSDV and correlated with an increase in the abundance of siRNAs derived from RBSDV. The results of this study suggest that reovirus replication in its insect vector potentially induces an RNAi-mediated antiviral response.

  19. Dissipation caused by a vorticity field and generation of singularities in Madelung fluid

    Energy Technology Data Exchange (ETDEWEB)

    Caliari, M [Faculty of Mathematical, Physical and Natural Science, University of Verona (Italy); Inverso, G [Elvis Elettronica, Padova (Italy); Morato, L M [Faculty of Mathematical, Physical and Natural Science, University of Verona (Italy)


    We consider a generalization of Madelung fluid equations, which was derived in the 1980s by means of a pathwise stochastic calculus of variations with the classical action functional. At variance with the original ones, the new equations allow us to consider velocity fields with vorticity. Such a vorticity causes dissipation of energy and it may concentrate, asymptotically, in the zeros of the density of the fluid. We study, by means of numerical methods, some Cauchy problems for the bidimensional symmetric harmonic oscillator and observe the generation of zeros of the density and concentration of the vorticity close to central lines and cylindrical sheets. Moreover, keeping the same initial data, we perturb the harmonic potential by a term proportional to the density of the fluid, thus obtaining an extension with vorticity of the Gross-Pitaevskii equation, and observe analogous behaviours.

  20. The Born-Infeld vortices induced from a generalized Higgs mechanism. (United States)

    Han, Xiaosen


    We construct self-dual Born-Infeld vortices induced from a generalized Higgs mechanism. Two specific models of the theory are of focused interest where the Higgs potential is either of a | ϕ | 4 - or | ϕ | 6 -type. For the | ϕ | 4 -model, we obtain a sharp existence and uniqueness theorem for doubly periodic and planar vortices. For doubly periodic solutions, a necessary and sufficient condition for the existence is explicitly derived in terms of the vortex number, the Born-Infeld parameter, and the size of the periodic lattice domain. For the | ϕ | 6 -model, we show that both topological and non-topological vortices are present. This new phenomenon distinguishes the model from the classical Born-Infeld-Higgs theory studied earlier in the literature. A series of results regarding doubly periodic, topological, and non-topological vortices in the | ϕ | 6 -model are also established.

  1. Derivation, Expansion, and Motor Neuron Differentiation of Human-Induced Pluripotent Stem Cells with Non-Integrating Episomal Vectors and a Defined Xenogeneic-free Culture System. (United States)

    Hu, Wentao; He, Yongpei; Xiong, Yongjie; Lu, Hong; Chen, Hong; Hou, Limin; Qiu, Zhandong; Fang, Yu; Zhang, Suming


    Induced pluripotent stem cells (iPSCs) generated from patient-derived somatic cells provides the opportunity for model development in order to study patient-specific disease states with the potential for drug discovery. However, use of lentivirus and exposure of iPSCs to animal-derived products limit their therapeutic utility and affect lineage differentiation and subsequent downstream functionality of iPSC derivatives. Within the context of this study, we describe a simple and practical protocol enabling the efficient reprogramming of terminally differentiated adult fibroblasts into integration-free human iPSCs (hiPSCs) using a combination of episomal plasmids with small molecules (SMs). Using this approach, there was a 10-fold increase in reprogramming efficiency over single plasmid vector-based methods. We obtained approximately 100 iPSCs colonies from 1 × 10(5) human adult dermal fibroblasts (HADFs) and achieved approximately 0.1% reprogramming efficiencies. Concurrently, we developed a highly conducive culture system using xeno-free media and human vitronectin. The resulting hiPSCs were free of DNA integration and had completely lost episomal vectors, maintained long-term self-renewal, featured a normal karyotype, expressed pluripotent stem cell markers, and possessed the capability of differentiating into components of all three germ layers in vivo. Finally, we demonstrate that the integration-free hiPSCs could be differentiated into motor neurons under xeno-free culture conditions. This induction method will promote the derivation of patient-specific integration-free and xeno-free iPSCs and improve the strategy for motor neuron derivation. Our approach provides a useful tool for human disease models, drug screen, and clinical applications.

  2. A vorticity budget for the Gulf Stream (United States)

    Le Bras, Isabela; Toole, John


    We develop a depth-averaged vorticity budget framework to diagnose the dynamical balance of the Gulf Stream, and apply this framework to observations and the ECCO state estimate (Wunsch and Heimbach 2013) above the thermocline in the subtropical North Atlantic. Using the hydrographic and ADCP data along the WOCE/CLIVAR section A22 and a variety of wind stress data products, we find that the advective vorticity flux out of the western region is on the same order as the wind stress forcing over the eastern portion of the gyre. This is consistent with a large-scale balance between a negative source of vorticity from wind stress forcing and a positive source of vorticity in the western region. Additionally, the form of the vorticity flux indicates that the Gulf Stream has a significant inertial component. In the ECCO state estimate, we diagnose a seasonal cycle in advective vorticity flux across a meridional section associated with seasonal fluctuations in Gulf Stream transport. This vorticity flux is forced by wind stress over the eastern subtropical North Atlantic and balanced by lateral friction with the western boundary. The lateral friction in ECCO is a necessary parameterization of smaller scale processes that occur in the real ocean, and quantifying these remains an open and interesting question. This simplified framework provides a means to interpret large scale ocean dynamics. In our application, it points to wind stress forcing over the subtropical North Altantic as an important regulator of the Gulf Stream and hence the climate system.

  3. Can symmetry transitions of complex fields enable 3-d control of fluid vorticity?

    Energy Technology Data Exchange (ETDEWEB)

    Martin, James E. [Sandia National Laboratories (SNL-NM), Albuquerque, NM (United States); Solis, Kyle Jameson [Sandia National Laboratories (SNL-NM), Albuquerque, NM (United States)


    Methods of inducing vigorous noncontact fluid flow are important to technologies involving heat and mass transfer and fluid mixing, since they eliminate the need for moving parts, pipes and seals, all of which compromise system reliability. Unfortunately, traditional noncontact flow methods are few, and have limitations of their own. We have discovered two classes of fields that can induce fluid vorticity without requiring either gravity or a thermal gradient. The first class we call Symmetry-Breaking Rational Fields. These are triaxial fields comprised of three orthogonal components, two ac and one dc. The second class is Rational Triad Fields, which differ in that all three components are alternating. In this report we quantify the induced vorticity for a wide variety of fields and consider symmetry transitions between these field types. These transitions give rise to orbiting vorticity vectors, a technology for non-contact, non-stationary fluid mixing.

  4. An Eulerian/Lagrangian coupling procedure for three-dimensional vortical flows (United States)

    Felici, Helene M.; Drela, Mark


    A coupled Eulerian/Lagrangian method is presented for the reduction of numerical diffusion observed in solutions of 3D vortical flows using standard Eulerian finite-volume time-marching procedures. A Lagrangian particle tracking method, added to the Eulerian time-marching procedure, provides a correction of the Eulerian solution. In turn, the Eulerian solution is used to integrate the Lagrangian state-vector along the particles trajectories. While the Eulerian solution ensures the conservation of mass and sets the pressure field, the particle markers describe accurately the convection properties and enhance the vorticity and entropy capturing capabilities of the Eulerian solver. The Eulerian/Lagrangian coupling strategies are discussed and the combined scheme is tested on a constant stagnation pressure flow in a 90 deg bend and on a swirling pipe flow. As the numerical diffusion is reduced when using the Lagrangian correction, a vorticity gradient augmentation is identified as a basic problem of this inviscid calculation.

  5. Correlations between Abelian monopoles and center vortices

    Energy Technology Data Exchange (ETDEWEB)

    Hosseini Nejad, Seyed Mohsen, E-mail:; Deldar, Sedigheh, E-mail:


    We study the correlations between center vortices and Abelian monopoles for SU(3) gauge group. Combining fractional fluxes of monopoles, center vortex fluxes are constructed in the thick center vortex model. Calculating the potentials induced by fractional fluxes constructing the center vortex flux in a thick center vortex-like model and comparing with the potential induced by center vortices, we observe an attraction between fractional fluxes of monopoles constructing the center vortex flux. We conclude that the center vortex flux is stable, as expected. In addition, we show that adding a contribution of the monopole-antimonopole pairs in the potentials induced by center vortices ruins the Casimir scaling at intermediate regime.

  6. Vortices and turbulence in trapped atomic condensates (United States)

    White, Angela C.; Anderson, Brian P.; Bagnato, Vanderlei S.


    After more than a decade of experiments generating and studying the physics of quantized vortices in atomic gas Bose–Einstein condensates, research is beginning to focus on the roles of vortices in quantum turbulence, as well as other measures of quantum turbulence in atomic condensates. Such research directions have the potential to uncover new insights into quantum turbulence, vortices, and superfluidity and also explore the similarities and differences between quantum and classical turbulence in entirely new settings. Here we present a critical assessment of theoretical and experimental studies in this emerging field of quantum turbulence in atomic condensates. PMID:24704880

  7. A new adenovirus based vaccine vector expressing an Eimeria tenella derived TLR agonist improves cellular immune responses to an antigenic target.

    Directory of Open Access Journals (Sweden)

    Daniel M Appledorn


    Full Text Available Adenoviral based vectors remain promising vaccine platforms for use against numerous pathogens, including HIV. Recent vaccine trials utilizing Adenovirus based vaccines expressing HIV antigens confirmed induction of cellular immune responses, but these responses failed to prevent HIV infections in vaccinees. This illustrates the need to develop vaccine formulations capable of generating more potent T-cell responses to HIV antigens, such as HIV-Gag, since robust immune responses to this antigen correlate with improved outcomes in long-term non-progressor HIV infected individuals.In this study we designed a novel vaccine strategy utilizing an Ad-based vector expressing a potent TLR agonist derived from Eimeria tenella as an adjuvant to improve immune responses from a [E1-]Ad-based HIV-Gag vaccine. Our results confirm that expression of rEA elicits significantly increased TLR mediated innate immune responses as measured by the influx of plasma cytokines and chemokines, and activation of innate immune responding cells. Furthermore, our data show that the quantity and quality of HIV-Gag specific CD8(+ and CD8(- T-cell responses were significantly improved when coupled with rEA expression. These responses also correlated with a significantly increased number of HIV-Gag derived epitopes being recognized by host T cells. Finally, functional assays confirmed that rEA expression significantly improved antigen specific CTL responses, in vivo. Moreover, we show that these improved responses were dependent upon improved TLR pathway interactions.The data presented in this study illustrate the potential utility of Ad-based vectors expressing TLR agonists to improve clinical outcomes dependent upon induction of robust, antigen specific immune responses.

  8. Modulation of CD8+ T cell responses to AAV vectors with IgG-derived MHC class II epitopes (United States)

    Hui, Daniel J; Basner-Tschakarjan, Etiena; Chen, Yifeng; Davidson, Robert J; Buchlis, George; Yazicioglu, Mustafa; Pien, Gary C; Finn, Jonathan D; Haurigot, Virginia; Tai, Alex; Scott, David W; Cousens, Leslie P; Zhou, Shangzhen; De Groot, Anne S; Mingozzi, Federico


    Immune responses directed against viral capsid proteins constitute a main safety concern in the use of adeno-associated virus (AAV) as gene transfer vectors in humans. Pharmacological immunosuppression has been proposed as a solution to the problem; however, the approach suffers from several potential limitations. Using MHC class II epitopes initially identified within human IgG, named Tregitopes, we showed that it is possible to modulate CD8+ T cell responses to several viral antigens in vitro. We showed that incubation of peripheral blood mononuclear cells with these epitopes triggers proliferation of CD4+CD25+FoxP3+ T cells that suppress killing of target cells loaded with MHC class I antigens in an antigen-specific fashion, through a mechanism that seems to require cell-to-cell contact. Expression of a construct encoding for the AAV capsid structural protein fused to Tregitopes resulted in reduction of CD8+ T cell reactivity against the AAV capsid following immunization with an adenoviral vector expressing capsid. This was accompanied by an increase in frequency of CD4+CD25+FoxP3+ T cells in spleens and lower levels of inflammatory infiltrates in injected tissues. This proof-of-concept study demonstrates modulation of CD8+ T cell reactivity to an antigen using regulatory T cell epitopes is possible. PMID:23857231

  9. Modulation of CD8+ T cell responses to AAV vectors with IgG-derived MHC class II epitopes. (United States)

    Hui, Daniel J; Basner-Tschakarjan, Etiena; Chen, Yifeng; Davidson, Robert J; Buchlis, George; Yazicioglu, Mustafa; Pien, Gary C; Finn, Jonathan D; Haurigot, Virginia; Tai, Alex; Scott, David W; Cousens, Leslie P; Zhou, Shangzhen; De Groot, Anne S; Mingozzi, Federico


    Immune responses directed against viral capsid proteins constitute a main safety concern in the use of adeno-associated virus (AAV) as gene transfer vectors in humans. Pharmacological immunosuppression has been proposed as a solution to the problem; however, the approach suffers from several potential limitations. Using MHC class II epitopes initially identified within human IgG, named Tregitopes, we showed that it is possible to modulate CD8+ T cell responses to several viral antigens in vitro. We showed that incubation of peripheral blood mononuclear cells with these epitopes triggers proliferation of CD4+CD25+FoxP3+ T cells that suppress killing of target cells loaded with MHC class I antigens in an antigen-specific fashion, through a mechanism that seems to require cell-to-cell contact. Expression of a construct encoding for the AAV capsid structural protein fused to Tregitopes resulted in reduction of CD8+ T cell reactivity against the AAV capsid following immunization with an adenoviral vector expressing capsid. This was accompanied by an increase in frequency of CD4+CD25+FoxP3+ T cells in spleens and lower levels of inflammatory infiltrates in injected tissues. This proof-of-concept study demonstrates modulation of CD8+ T cell reactivity to an antigen using regulatory T cell epitopes is possible.

  10. Topological charge algebra of optical vortices in nonlinear interactions (United States)

    Shutova, Mariia; Zhdanova, Alexandra; Bahari, Aysan; Zhi, Miaochan; Sokolov, Alexei


    Optical vortices find their use in multiple areas of research and technology; in particular, they provide an opportunity to generate short-pulse spatially-structured optical beams, which can be used to study ultrafast processes. In our work, we explore interactions of femtosecond optical vortices in nonlinear crystals. We investigate the transfer of orbital angular momentum among multiple (applied and generated) beams involved in a coherent Raman interaction. We use a liquid crystal light modulator to shape the applied pump and Stokes beams into optical vortices with various integer values of topological charge, and cross them in a Raman-active crystal to produce multiple Stokes and anti-Stokes sidebands. We then examine the transfer of optical angular momentum into each sideband and find that it follows a certain law that can be derived from angular momentum conservation for created and annihilated photons, or equivalently, from phase-matching considerations for the interacting beams. Presenter is supported by the Herman F. Heep and Minnie Belle Heep Texas A&M University Endowed Fund administered by the Texas A&M Foundation

  11. Chaotic vortical flows and their manifestations

    Directory of Open Access Journals (Sweden)

    Baznat M.


    Full Text Available We study vorticity and hydrodynamic helicity in semi-peripheral heavy-ion collisions using the kinetic model of Quark-Gluon Strings. The angular momentum, which is a source of P-odd observables, is preserved with a good accuracy. We observe formation of the specific toroidal structures of the vorticity field. Their existence, accompanied by the strange chemical potential, is mirrored in the polarization of hyperons of the percent order.

  12. Complete active space configuration interaction from state-averaged configuration interaction singles natural orbitals: Analytic first derivatives and derivative coupling vectors. (United States)

    Fales, B Scott; Shu, Yinan; Levine, Benjamin G; Hohenstein, Edward G


    A new complete active space configuration interaction (CASCI) method was recently introduced that uses state-averaged natural orbitals from the configuration interaction singles method (configuration interaction singles natural orbital CASCI, CISNO-CASCI). This method has been shown to perform as well or better than state-averaged complete active space self-consistent field for a variety of systems. However, further development and testing of this method have been limited by the lack of available analytic first derivatives of the CISNO-CASCI energy as well as the derivative coupling between electronic states. In the present work, we present a Lagrangian-based formulation of these derivatives as well as a highly efficient implementation of the resulting equations accelerated with graphical processing units. We demonstrate that the CISNO-CASCI method is practical for dynamical simulations of photochemical processes in molecular systems containing hundreds of atoms.

  13. Vortices and vortex lattices in quantum ferrofluids. (United States)

    Martin, A M; Marchant, N G; O'Dell, D H J; Parker, N G


    The experimental realization of quantum-degenerate Bose gases made of atoms with sizeable magnetic dipole moments has created a new type of fluid, known as a quantum ferrofluid, which combines the extraordinary properties of superfluidity and ferrofluidity. A hallmark of superfluids is that they are constrained to rotate through vortices with quantized circulation. In quantum ferrofluids the long-range dipolar interactions add new ingredients by inducing magnetostriction and instabilities, and also affect the structural properties of vortices and vortex lattices. Here we give a review of the theory of vortices in dipolar Bose-Einstein condensates, exploring the interplay of magnetism with vorticity and contrasting this with the established behaviour in non-dipolar condensates. We cover single vortex solutions, including structure, energy and stability, vortex pairs, including interactions and dynamics, and also vortex lattices. Our discussion is founded on the mean-field theory provided by the dipolar Gross-Pitaevskii equation, ranging from analytic treatments based on the Thomas-Fermi (hydrodynamic) and variational approaches to full numerical simulations. Routes for generating vortices in dipolar condensates are discussed, with particular attention paid to rotating condensates, where surface instabilities drive the nucleation of vortices, and lead to the emergence of rich and varied vortex lattice structures. We also present an outlook, including potential extensions to degenerate Fermi gases, quantum Hall physics, toroidal systems and the Berezinskii-Kosterlitz-Thouless transition.

  14. Generation and propagation of optical vortices (United States)

    Rozas, David

    Optical vortices are singularities in phase fronts of laser beams. They are characterized by a dark core whose size (relative to the size of the background beam) may dramatically affect their behavior upon propagation. Previously, only large-core vortices have been extensively studied. The object of the research presented in this dissertation was to explore ways of generating small-core optical vortices (also called optical vortex filaments ), and to examine their propagation using analytical, numerical and experimental methods. Computer-generated holography enabled us to create arbitrary distributions of optical vortex filaments for experimental exploration. Hydrodynamic analogies were used to develop an heuristic model which described the dependence of vortex motion on other vortices and the background beam, both qualitatively and quantitatively. We predicted that pair of optical vortex filaments will rotate with angular rates inversely proportional to their separation distance (just like vortices in a fluid). We also reported the first experimental observation of this novel fluid-like effect. It was found, however, that upon propagation in linear media, the fluid-like rotation was not sustained owing to the overlap of diffracting vortex cores. Further numerical studies and experiments showed that rotation angle may be enhanced in nonlinear self-defocusing media. The results presented in this thesis offer us a better understanding of dynamics of propagating vortices which may result in applications in optical switching, optical data storage, manipulation of micro-particles and optical limiting for eye protection.

  15. Simultaneous Velocity and Vorticity Measurement in Turbulence (United States)

    Wu, Huixuan; Xu, Haitao; Bodenschatz, Eberhard


    A new paradigm of simultaneous velocity and vorticity measurement is developed to study turbulence. Instead of deducing vorticity from velocities measured at neighboring points, this innovative approach detects the translations and rotations of micro-sized particles directly. These hydrogel particles are spherical, transparent, and encapsulate micro-mirrors. This method outstands conventional ones, e.g., hotwire arrays or PIV because its spatial resolution is much higher. It does not require a non-zero mean flow, and it can provide all three vorticity components, which is not available from planar PIV data. Its principle is to illuminate the mirror and utilize the variation of the reflection directions to deduce the local flow vorticity. Meanwhile, the particle position is recorded as in normal particle tracking. Therefore, the velocity and vorticity of a particle can be obtained simultaneously in Lagrangian framework. The authors have made benchmark experiments to evaluate this novel method in Taylor Couette flows. The results show that the instantaneous vorticity measurement is as accurate as 3%. We are now setting up a von Karman disk pair device to study the turbulent flow. This novel technique will provide unprecedented information of high Reynolds number turbulence. The first author thanks the Alexander von Humboldt Foundation.

  16. Direct simulation of isolated elliptic vortices and of their radiated noise

    Energy Technology Data Exchange (ETDEWEB)

    Barre, Sebastien [Ecole Centrale de Lyon, Laboratoire de Mecanique des Fluides et d' Acoustique, UMR CNRS 5509, Ecully Cedex (France); Aircraft and Stores Engineering Technical Directorate, Dassault-Aviation, St Cloud Cedex (France); Bogey, Christophe; Bailly, Christophe [Ecole Centrale de Lyon, Laboratoire de Mecanique des Fluides et d' Acoustique, UMR CNRS 5509, Ecully Cedex (France)


    The aerodynamic evolution and the acoustic radiation of elliptic vortices with various aspect ratios and moderate Mach numbers are investigated by solving numerically the full compressible Navier-Stokes equations. Three behaviours are observed according to the aspect ratio {sigma}=a/b where a and b are the major and minor semi-axes of the vortices. At the small aspect ratio {sigma}=1.2, the vortex rotates at a constant angular velocity and radiates like a rotating quadrupole. At the moderate aspect ratio {sigma}=5, the vortex is initially unstable. However the growth of instability waves is inhibited by the return to axisymmetry which decreases its aspect ratio. The noise level becomes lower with time and the radiation frequency increases. For vortices with larger aspect ratios {sigma} {>=}6, the return to axisymmetry does not occur quickly enough to stop the growth of instabilities, which splits the vortices. Various mergers are then found to occur. For instance in the case {sigma}=6, several successive switches between an elliptic state and a configuration of two co-rotating vortices are observed. The present results show that the initial value of the aspect ratio yields the relative weight between the return to axisymmetry which stabilizes the vortex and the growth of instabilities which tends to split it. Moreover the noise generated by the vortices is also calculated using the analytical solution derived by Howe (J. Fluid Mech. 71:625-673, 1975) and is compared with the reference solution provided by the direct computation. This solution is found to be valid for {sigma}=1.2. An extended solution is proposed for higher aspect ratios. Finally, the pressure field appears weakly affected by the switches between the two unstable configurations in the case {sigma}=6, which underlines the difficulty to detect the split or the merger of vortices from the radiated pressure. This study also shows that elliptic vortices can be used as a basic configuration of aerodynamic

  17. Geometric phase mediated topological transport of sound vortices

    CERN Document Server

    Wang, Shubo; Chan, C T


    When a physical system undergoes a cyclic evolution, a non-integrable phase can arise in addition to the normal dynamical phase. This phase, depending only on the geometry of the path traversed in the parameter space and hence named geometric phase, has profound impact in both classical and quantum physics, leading to exotic phenomena such as electron weak anti-localization and light spin-Hall effect. Experimental observations of the geometric phase effect in classical system are typically realized using vector waves such as light characterized by a polarization. We show here that such an effect can also be realized in scalar wave systems such as sound wave. Using a helical hollow waveguide, we show that the geometric phase effect associated with the transportation of sound vortices, i.e. sound wave carrying intrinsic orbital angular momentum, can serve as a potential mechanism to control the flow of sound vortices with different topological charges, resulting in geometric phase-based sound vortex filters.

  18. Modelling the distributions of Culicoides bluetongue virus vectors in Sicily in relation to satellite-derived climate variables. (United States)

    Purse, B V; Tatem, A J; Caracappa, S; Rogers, D J; Mellor, P S; Baylis, M; Torina, A


    Surveillance data from 268 sites in Sicily are used to develop climatic models for prediction of the distribution of the main European bluetongue virus (BTV) vector Culicoides imicola Kieffer (Diptera: Ceratopogonidae) and of potential novel vectors, Culicoides pulicaris Linnaeus, Culicoides obsoletus group Meigen and Culicoides newsteadi Austen. The models containing the 'best' climatic predictors of distribution for each species, were selected from combinations of 40 temporally Fourier-processed remotely sensed variables and altitude at a 1 km spatial resolution using discriminant analysis. Kappa values of around 0.6 for all species models indicated substantial levels of agreement between model predictions and observed data. Whilst the distributions of C. obsoletus group and C. newsteadi were predicted by temperature variables, those of C. pulicaris and C. imicola were determined mainly by normalized difference vegetation index (NDVI), a variable correlated with soil moisture and vegetation biomass and productivity. These models were used to predict species presence in unsampled pixels across Italy and for C. imicola across Europe and North Africa. The predicted continuous presence of C. pulicaris along the appenine mountains, from north to south Italy, suggests BTV transmission may be possible in a large proportion of this region and that seasonal transhumance (seasonal movement of livestock between upland and lowland pastures) even in C. imicola-free areas should not generally be considered safe. The predicted distribution of C. imicola distribution shows substantial agreement with observed surveillance data from Greece and Iberia (including the Balearics) and parts of mainland Italy (Lazio, Tuscany and areas of the Ionian coast) but is generally much more restricted than the observed distribution (in Sardinia, Corsica and Morocco). The low number of presence sites for C. imicola in Sicily meant that only a restricted range of potential C. imicola habitats were

  19. Evaluation of an AAV2-based rapamycin-regulated glial cell line-derived neurotrophic factor (GDNF expression vector system.

    Directory of Open Access Journals (Sweden)

    Piotr Hadaczek

    Full Text Available Effective regulation of transgene product in anatomically circumscribed brain tissue is dependent on the pharmacokinetics of the regulating agent, the kinetics of transcriptional activation and degradation of the transgene product. We evaluated rapamycin-regulated AAV2-GDNF expression in the rat brain (striatum. Regulated (a dual-component system: AAV2-FBZhGDNF + AAV2-TF1Nc and constitutive (CMV-driven expression vectors were compared. Constitutively active AAV2-GDNF directed stable GDNF expression in a dose-dependent manner and it increased for the first month, thereafter reaching a plateau that was maintained over a further 3 months. For the AAV2-regGDNF, rapamycin was administered in a 3-days on/4-days off cycle. Intraperitoneal, oral, and direct brain delivery (CED of rapamycin were evaluated. Two cycles of rapamycin at an intraperitoneal dose of 10 mg/kg gave the highest GDNF level (2.75±0.01 ng/mg protein. Six cycles at 3 mg/kg resulted in lower GDNF values (1.36±0.3 ng/mg protein. Interestingly, CED of rapamycin into the brain at a very low dose (50 ng induced GDNF levels comparable to a 6-week intraperitoneal rapamycin cycle. This study demonstrates the effectiveness of rapamycin regulation in the CNS. However, the kinetics of the transgene in brain tissue, the regulator dosing amount and schedule are critical parameters that influence the kinetics of accumulation and zenith of the encoded transgene product.

  20. Enhanced survival of the LINCL mouse following CLN2 gene transfer using the rh.10 rhesus macaque-derived adeno-associated virus vector. (United States)

    Sondhi, Dolan; Hackett, Neil R; Peterson, Daniel A; Stratton, Jamie; Baad, Michael; Travis, Kelly M; Wilson, James M; Crystal, Ronald G


    Late infantile neuronal ceroid lipofuscinosis (LINCL) is a lysosomal storage disorder caused by mutations in the CLN2 gene and a deficiency of tripeptidyl peptidase I (TPP-I). Prior studies with adeno-associated virus (AAV) serotype 2 or 5 mediated transfer of the CLN2 complementary DNA to the central nervous system (CNS) of CLN2(-/-) mice cleared CNS storage granules, but provided no improvement in the phenotype or survival of this model of LINCL. In this study, AAV serotypes (AAV2, AAV5, AAV8, and AAVrh.10) were compared for the delivery of the same CLN2 expression cassette. AAVrh.10, derived from rhesus macaque, provided the highest TPP-I level and maximum spread beyond the site of injection. The AAVrh.10-based vector functioned equally well in naive rats and in rats previously immunized against human serotypes of AAV. When administered to the CNS of CLN2(-/-) mice, the AAVrh.10CLN2 vector provided widespread TPP-I activity comparable to that in the wild-type mice. Importantly, the AAVrh.10CLN2-treated CLN2(-/-) mice had significant reduction in CNS storage granules and demonstrated improvement in gait, nest-making abilities, seizures, balance beam function, and grip strength, as well as having a survival advantage.

  1. Inhibition of histone deacetylation in 293GPG packaging cell line improves the production of self-inactivating MLV-derived retroviral vectors

    Directory of Open Access Journals (Sweden)

    Crosato Milena


    Full Text Available Abstract Background Self-inactivating retroviral vectors (SIN are often associated with very low titers. Promoter elements embedded within SIN designs may suppress transcription of packageable retroviral RNA which in turn results in titer reduction. We tested whether this dominant-negative effect involves histone acetylation state. We designed an MLV-derived SIN vector using the cytomegalovirus immediate early enhancer-promoter (CMVIE as an embedded internal promoter (SINCMV and transfected the pantropic 293GPG packaging cell line. Results The SINCMV retroviral producer had uniformly very low titers (~10,000 infectious retroparticles per ml. Northern blot showed low levels of expression of retroviral mRNA in producer cells in particular that of packageable RNA transcript. Treatment of the producers with the histone deacetylase (HDAC inhibitors sodium butyrate and trichostatin A reversed transcriptional suppression and resulted in an average 106.3 ± 4.6 – fold (P = 0.002 and 15.5 ± 1.3 – fold increase in titer (P = 0.008, respectively. A histone gel assay confirmed increased histone acetylation in treated producer cells. Conclusion These results show that SIN retrovectors incorporating strong internal promoters such as CMVIE, are susceptible to transcriptional silencing and that treatment of the producer cells with HDAC inhibitors can overcome this blockade suggesting that histone deacetylation is implicated in the mechanism of transcriptional suppression.

  2. Cubic nonlinear Schrödinger equation with vorticity (United States)

    Caliari, M.; Loffredo, M. I.; Morato, L. M.; Zuccher, S.


    In this paper, we introduce a new class of nonlinear Schrödinger equations (NLSEs), with an electromagnetic potential (\\mathcal A,\\Phi) , both depending on the wavefunction Ψ. The scalar potential Φ depends on |Ψ|2, whereas the vector potential \\mathcal A satisfies the equation of magnetohydrodynamics with coefficient depending on Ψ. In Madelung variables, the velocity field comes to be not irrotational in general and we prove that the vorticity induces dissipation, until the dynamical equilibrium is reached. The expression of the rate of dissipation is common to all NLSEs in the class. We show that they are a particular case of the one-particle dynamics out of dynamical equilibrium for a system of N identical interacting Bose particles, as recently described within stochastic quantization by Lagrangian variational principle. The cubic case is discussed in particular. Results of numerical experiments for rotational excitations of the ground state in a finite two-dimensional trap with harmonic potential are reported.

  3. Peptides derived from evolutionarily conserved domains in Beclin-1 and Beclin-2 enhance the entry of lentiviral vectors into human cells. (United States)

    Majdoul, Saliha; Cosette, Jeremie; Seye, Ababacar K; Bernard, Eric; Frin, Sophie; Holic, Nathalie; Chazal, Nathalie; Briant, Laurence; Espert, Lucile; Galy, Anne; Fenard, David


    Autophagy-related proteins such as Beclin-1 are involved in an array of complex processes, including antiviral responses, and may also modulate the efficiency of gene therapy viral vectors. The Tat-Beclin-1 (TB1) peptide has been reported as an autophagy-inducing factor inhibiting the replication of pathogens such as HIV, type 1 (HIV-1). However, autophagy-related proteins are also essential for the early steps of HIV-1 infection. Therefore, we examined the effects of the Beclin-1 evolutionarily conserved domain in TB1 on viral transduction and autophagy in single-round HIV infection or with nonreplicative HIV-1-derived lentiviral vectors. TB1 enhanced transduction with various pseudotypes but without inducing the autophagy process. TB1 augmented the transduction of human CD34+ hematopoietic stem/progenitor cells while maintaining their capacity to engraft in vivo into humanized mice. TB1 was as effective as other transduction additives and functioned by enhancing the adhesion and fusion of viral particles with target cells but not their aggregation. We also found that the N-terminal L1 loop was critical for TB1 transduction-enhancing activity. Interestingly, the Tat-Beclin-2 (TB2) peptide, derived from the human Beclin-2 protein, was even more potent than TB1 in promoting viral transduction and infection. Taken together, our findings suggest that the TB1 and TB2 peptides enhance the viral entry step. Tat-Beclin peptides therefore represent a new family of viral transduction enhancers for potential use in gene therapy. © 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

  4. A novel dendrimer based on poly (L-glutamic acid) derivatives as an efficient and biocompatible gene delivery vector

    Energy Technology Data Exchange (ETDEWEB)

    Zeng Xin; Pan Shirong; Wang Chi; Wen Yuting; Wu Hongmei; Wang Cuifeng; Wu Chuanbin; Feng Min [School of Pharmaceutical Sciences and the First Affiliated Hospital, Sun Yat-sen University, 80 Zhongshan Road II, Guangzhou 510080 (China); Li Jie, E-mail:, E-mail: [Department of Gastrointestinal Surgery, The Third Affiliated Hospital of Sun Yat-sen University, 80 Zhongshan Road II, Guangzhou 510080 (China)


    Non-viral gene delivery systems based on cationic polymers have faced limitations related to their relative low gene transfer efficiency, cytotoxicity and system instability in vivo. In this paper, a flexible and pompon-like dendrimer composed of poly (amidoamine) (PAMAM) G4.0 as the inner core and poly (L-glutamic acid) grafted low-molecular-weight polyethylenimine (PLGE) as the surrounding multiple arms was synthesized (MGI dendrimer). The novel MGI dendrimer was designed to combine the merits of size-controlled PAMAM G4.0 and the low toxicity and flexible chains of PLGE. In phosphate-buffered saline dispersions the well-defined DNA/MGI complex above a N/P ratio of 30 showed good stability with particle sizes of approximately 200 nm and a comparatively low polydispersity index. However, the particle size of the DNA/25 kDa polyethylenimine (DNA/PEI 25K) complex was larger than 700 nm under the same salt conditions. The shielding of the compact amino groups at the periphery of flexible PAMAM and biocompatible PLGE chains in MGI resulted in a dramatic decrease of the cytotoxicity compared to native PAMAM G4.0 dendrimer. The in vitro transfection efficiency of DNA/MGI dendrimer complex was higher than that of PAMAM G4.0 dendrimer. Importantly, in serum-containing medium, DNA/MGI complexes at their optimal N/P ratio maintained the same high levels of transfection efficiency as in serum-free medium, while the transfection efficiency of native PAMAM G4.0, PEI 25K and Lipofectamine 2000 were sharply decreased. In vivo gene delivery of pVEGF165/MGI complex into balloon-injured rabbit carotid arteries resulted in significant inhibition of restenosis by increasing VEGF165 expression in local vessels. Therefore, the pompon-like MGI dendrimer may be a promising vector candidate for efficient gene delivery in vivo.

  5. Characterization of lentiviral vector production using microwell suspension cultures of HEK293T-derived producer cells. (United States)

    Guy, Heather M; McCloskey, Laura; Lye, Gary J; Mitrophanous, Kyriacos A; Mukhopadhyay, Tarit K


    ProSavin(®) is a lentiviral vector (LV)-based gene therapy for Parkinson's disease. ProSavin(®) is currently in a Phase I/II clinical trial using material that was generated by transient transfection of adherent human embryonic kidney (HEK)293T cells. For future large-scale productions of ProSavin(®), we have previously reported the development and characterization of two inducible producer cell lines, termed PS5.8 and PS46.2. PS46.2 has been successfully adapted to grow in suspension cultures. The present study describes the creation of a small-scale (combined with statistical design of experiments (DoE) techniques to enable rapid characterization of the process conditions that impact cell growth and LV production. The effects of postinduction period, microwell liquid fill volume, and concentration of inducer (doxycycline) on ProSavin(®) titer and the particle:infectivity (P:I) ratio was investigated using three rounds of DoE, in order to identify appropriate factor ranges and optimize production conditions. We identified an optimal "harvest window" between approximately 26-46 hr within which maximal titers of around 6×10(4) transducing units (TU)/ml were obtained (an approximately 30-fold improvement compared to starting microwell conditions), providing that the fill volume was maintained at or below 1 ml and the doxycycline concentration was at least 1.0 μg/ml. Insights from the microwell studies were subsequently used to rapidly establish operating conditions for ProSavin(®) production in a 0.5-L wave bioreactor culture. The information presented herein thus aids the design and evaluation of scalable production processes for LVs.

  6. Inhibition of core gene of HCV 3a genotype using synthetic and vector derived siRNAs. (United States)

    Khaliq, Saba; Jahan, Shah; Ijaz, Bushra; Ahmad, Waqar; Asad, Sultan; Pervaiz, Asim; Samreen, Baila; Khan, Mahwish; Hassan, Sajida


    Hepatitis C virus (HCV) is a major causative agent of liver associated diseases throughout the world, with genotype 3a responsible for most of the cases in Pakistan. Due to the limited efficiency of current therapy, RNA interference (RNAi) a novel regulatory and powerful silencing approach for molecular therapeutics through a sequence-specific RNA degradation process represents an alternative option. The current study was purposed to assess and explore the possibility of RNAi to silence the HCV-3a Core gene expression, which play complex role in regulation of cell growth and host genes expression essential for infectivity and disease progression. To identify the potent siRNA target sites, 5 small interfering RNAs (siRNAs) against Core gene were designed and in vitro transcribed after consensus sequence analysis of different HCV-3a isolates. Antiviral effects of siRNAs showed upto 80% inhibition of Core gene expression by different siRNAs into Huh-7 cells as compared with Mock transfected and control siRNAs treated cells. For long lasting effect of siRNAs, vector based short hairpin siRNAs (shRNAs) were designed and tested against HCV-3a Core which resulted in a similar pattern of inhibition on RNA and protein expression of HCV Core as synthetic siRNAs. Furthermore, the efficacy of cell culture tested siRNA and shRNA, were evaluated for inhibition of HCV replication in HCV infected serum inoculated Huh-7 cells and a significant decrease in HCV viral copy number was observed. Our results support the possibility of using consensus siRNA and shRNA-based molecular therapy as a promising strategy in effective inhibition of HCV-3a genotype.

  7. A novel dendrimer based on poly (L-glutamic acid) derivatives as an efficient and biocompatible gene delivery vector (United States)

    Zeng, Xin; Pan, Shirong; Li, Jie; Wang, Chi; Wen, Yuting; Wu, Hongmei; Wang, Cuifeng; Wu, Chuanbin; Feng, Min


    Non-viral gene delivery systems based on cationic polymers have faced limitations related to their relative low gene transfer efficiency, cytotoxicity and system instability in vivo. In this paper, a flexible and pompon-like dendrimer composed of poly (amidoamine) (PAMAM) G4.0 as the inner core and poly (L-glutamic acid) grafted low-molecular-weight polyethylenimine (PLGE) as the surrounding multiple arms was synthesized (MGI dendrimer). The novel MGI dendrimer was designed to combine the merits of size-controlled PAMAM G4.0 and the low toxicity and flexible chains of PLGE. In phosphate-buffered saline dispersions the well-defined DNA/MGI complex above a N/P ratio of 30 showed good stability with particle sizes of approximately 200 nm and a comparatively low polydispersity index. However, the particle size of the DNA/25 kDa polyethylenimine (DNA/PEI 25K) complex was larger than 700 nm under the same salt conditions. The shielding of the compact amino groups at the periphery of flexible PAMAM and biocompatible PLGE chains in MGI resulted in a dramatic decrease of the cytotoxicity compared to native PAMAM G4.0 dendrimer. The in vitro transfection efficiency of DNA/MGI dendrimer complex was higher than that of PAMAM G4.0 dendrimer. Importantly, in serum-containing medium, DNA/MGI complexes at their optimal N/P ratio maintained the same high levels of transfection efficiency as in serum-free medium, while the transfection efficiency of native PAMAM G4.0, PEI 25K and Lipofectamine 2000 were sharply decreased. In vivo gene delivery of pVEGF165/MGI complex into balloon-injured rabbit carotid arteries resulted in significant inhibition of restenosis by increasing VEGF165 expression in local vessels. Therefore, the pompon-like MGI dendrimer may be a promising vector candidate for efficient gene delivery in vivo.

  8. Intracardiac Vortex Dynamics by High-Frame-Rate Doppler Vortography-In Vivo Comparison With Vector Flow Mapping and 4-D Flow MRI. (United States)

    Faurie, Julia; Baudet, Mathilde; Assi, Kondo Claude; Auger, Dominique; Gilbert, Guillaume; Tournoux, Francois; Garcia, Damien


    Recent studies have suggested that intracardiac vortex flow imaging could be of clinical interest to early diagnose the diastolic heart function. Doppler vortography has been introduced as a simple color Doppler method to detect and quantify intraventricular vortices. This method is able to locate a vortex core based on the recognition of an antisymmetric pattern in the Doppler velocity field. Because the heart is a fast-moving organ, high frame rates are needed to decipher the whole blood vortex dynamics during diastole. In this paper, we adapted the vortography method to high-frame-rate echocardiography using circular waves. Time-resolved Doppler vortography was first validated in vitro in an ideal forced vortex. We observed a strong correlation between the core vorticity determined by high-frame-rate vortography and the ground-truth vorticity. Vortography was also tested in vivo in ten healthy volunteers using high-frame-rate duplex ultrasonography. The main vortex that forms during left ventricular filling was tracked during two-three successive cardiac cycles, and its core vorticity was determined at a sampling rate up to 80 duplex images per heartbeat. Three echocardiographic apical views were evaluated. Vortography-derived vorticities were compared with those returned by the 2-D vector flow mapping approach. Comparison with 4-D flow magnetic resonance imaging was also performed in four of the ten volunteers. Strong intermethod agreements were observed when determining the peak vorticity during early filling. It is concluded that high-frame-rate Doppler vortography can accurately investigate the diastolic vortex dynamics.

  9. Stability of helical tip vortices in a rotor far wake

    DEFF Research Database (Denmark)

    Okulov, Valery; Sørensen, Jens Nørkær


    , corresponding to Rankine, Gaussian and Scully vortices, at radial extents ranging from the core radius of a tip vortex to several rotor radii. The analysis shows that the stability of tip vortices largely depends on the radial extent of the hub vorticity as well as on the type of vorticity distribution. As part......As a means of analysing the stability of the wake behind a multi-bladed rotor the stability of a multiplicity of helical vortices embedded in an assigned flow field is addressed. In the model the tip vortices in the far wake are approximated by infinitely long helical vortices with constant pitch...... and radius. The work is a further development of a model developed in Okulov (J. Fluid Mech., vol. 521, p. 319) in which the linear stability of N equally azimuthally spaced helical vortices was considered. In the present work the analysis is extended to include an assigned vorticity field due to root...

  10. Diagnostic study on the relation between ozone and vorticity potential

    Energy Technology Data Exchange (ETDEWEB)

    Abdel Basset, H. [Department of Astronomy and Meteorology, Faculty of Science, Al Azhar University, Nasr City, Cairo (Egypt); Gahein, A. [Egyptian Meteorological Authority, Cairo (Egypt)


    A diagnostic analysis of a Mediterranean system and the associated tropopause folding for the period 27 February to 10 March, 1987 is presented. Geopotential height, potential vorticity (PV) and relative humidity distributions were diagnosed. The analysis indicates clear correlation between the development of the cut-off low and the tropopause folding. A series of vertical cross-sections at the ends of the jet streaks demonstrated that a fold could be captured using potential vorticity and relative humidity. Q-vectors were employed to investigate vertical motion in the vicinity of the fold and showed the exact positions of descent corresponding to the fold along the entire length of the jet streak. The analysis also shows that the strong correlation between total ozone and column integrated vorticity potential holds well for all levels. As both quantities are integrals through the atmosphere, this result is consistent with, but does not prove, a high independent linear dependence between ozone and PV. More case studies are needed to assure the high linear dependence between ozone and PV. The maximum transport of ozone from the stratosphere to the troposphere is coinciding with the maximum developing system, and also with the maximum values of PV. [Spanish] Se presenta un analisis diagnostico de un Sistema mediterraneo y del pliegue de la tropopausa asociado durante el periodo del 27 de febrero al 10 de marzo de 1987. Se diagnosticaron la altitud neopotencial, el potencial de vorticidad y la distribucion de la humedad relativa. El analisis indica una correlacion clara entre el desarrollo de la baja segregada y el pliegue de la tropopausa. Una serie de cortes verticales en los extremos de las trazas del chorro demostraron que el pliegue puede ser capturado utilizando el potencial de vorticidad y la humedad relativa. Para investigar la movilidad vertical en la vecindad del pliegue se utilizaron vectores Q, y se demostraron las posiciones exactas de descenso

  11. Use of acoustic vortices in acoustic levitation

    DEFF Research Database (Denmark)

    Cutanda Henriquez, Vicente; Santillan, Arturo Orozco; Juhl, Peter Møller


    Acoustic fields are known to exert forces on the surfaces of objects. These forces are noticeable if the sound pressure is sufficiently high. Two phenomena where acoustic forces are relevant are: i) acoustic levitation, where strong standing waves can hold small objects at certain positions......, counterbalancing their weight, and ii) acoustic vortices, spinning sound fields that can impinge angular momentum and cause rotation of objects. In this contribution, both force-creating sound fields are studied by means of numerical simulations. The Boundary Element Method is employed to this end. The simulation...... of acoustical vortices uses an efficient numerical implementation based on the superposition of two orthogonal sound fields with a delay of 90° between them. It is shown that acoustic levitation and the use of acoustic vortices can be combined to manipulate objects in an efficient and controlled manner without...

  12. Dynamics of quantised vortices in superfluids

    CERN Document Server

    Sonin, Edouard B


    A comprehensive overview of the basic principles of vortex dynamics in superfluids, this book addresses the problems of vortex dynamics in all three superfluids available in laboratories (4He, 3He, and BEC of cold atoms) alongside discussions of the elasticity of vortices, forces on vortices, and vortex mass. Beginning with a summary of classical hydrodynamics, the book guides the reader through examinations of vortex dynamics from large scales to the microscopic scale. Topics such as vortex arrays in rotating superfluids, bound states in vortex cores and interaction of vortices with quasiparticles are discussed. The final chapter of the book considers implications of vortex dynamics to superfluid turbulence using simple scaling and symmetry arguments. Written from a unified point of view that avoids complicated mathematical approaches, this text is ideal for students and researchers working with vortex dynamics in superfluids, superconductors, magnetically ordered materials, neutron stars and cosmological mo...

  13. Fractional Vortices in Multi-Gap Superconductors (United States)

    Loh, Yen Lee; Kim, Monica; Kim, Ju H.


    Novel topological defects, known as fractional vortices, can occur in thin films of multi-gap superconductors. We study two-gap and three-gap superconducting films within a classical Ginzburg-Landau description, using numerical simulations and analytic approximations. In two-gap superconducting films, we find that the interband Josephson coupling J12 leads to an effective attraction between half-vortices, whereas the permeability parameter μ leads to an effective repulsion between half-vortices. We locate the phase boundary in (J12 , μ) space that marks the onset of spontaneous vortex fractionalization. We describe how the size of a fractional vortex increases as one goes deeper into the fractionalized phase. Our results suggest that coating a multi-gap superconducting film with a paramagnetic overlayer will enhance the tendency towards vortex fractionalization.

  14. Vorticity field measurement using digital inline holography (United States)

    Mallery, Kevin; Hong, Jiarong


    We demonstrate the direct measurement of a 3D vorticity field using digital inline holographic microscopy. Microfiber tracer particles are illuminated with a 532 nm continuous diode laser and imaged using a single CCD camera. The recorded holographic images are processed using a GPU-accelerated inverse problem approach to reconstruct the 3D structure of each microfiber in the imaged volume. The translation and rotation of each microfiber are measured using a time-resolved image sequence - yielding velocity and vorticity point measurements. The accuracy and limitations of this method are investigated using synthetic holograms. Measurements of solid body rotational flow are used to validate the accuracy of the technique under known flow conditions. The technique is further applied to a practical turbulent flow case for investigating its 3D velocity field and vorticity distribution.

  15. A note on the energy of relative equilibria of point vortices

    DEFF Research Database (Denmark)

    Aref, Hassan


    Analytical formulas are derived for the energy of simple relative equilibria of identical point vortices such as the regular polygons, both open and centered, and the various known configurations consisting of nested regular polygons with or without a vortex at the center. ©2007 American Institute...

  16. High-throughput sequencing as an effective approach in profiling small RNAs derived from a hairpin RNA expression vector in woody plants. (United States)

    Zhao, Dongyan; Song, Guo-Qing


    Hairpin RNA (hpRNA)-mediated gene silencing has proved to be an efficient approach to develop virus-resistant transgenic plants. To characterize small RNA molecules (sRNAs) derived from an hpRNA expression vector in transgenic cherry rootstock plants, we conducted small RNA sequencing of (1) a transgenic rootstock containing an inverted repeat of the partial coat protein of Prunus necrotic ring spot virus (PNRSV-hpRNA); (2) a nontransgenic rootstock; and (3) a PNRSV-infected sweet cherry plant. Analysis of the PNRSV sRNA pools indicated that 24-nt (nucleotide) small interfering RNAs (siRNAs) were the most prevalent sRNAs in the transgenic rootstock whereas the most abundant sRNAs in the PNRSV-infected nontransgenic rootstock were 21-nt siRNAs. In addition, the 24-nt siRNAs of the PNRSV-hpRNA were more abundant on the sense strand than those on the antisense strand in the transgenic rootstock. In contrast, preference in generating PNRSV sRNAs, ranging from 19-nt to 30-nt for sense and antisense strands, was not distinct in the PNRSV-infected nontransgenic sweet cherry. Taken together, this is the first report on profiling hpRNA-derived sRNAs in woody plants using high-throughput sequencing technology, which is an efficient way to verify the presence/absence, the abundance, and the sequence features of certain sRNAs. Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

  17. Targeted suicide gene therapy for glioma using human embryonic stem cell-derived neural stem cells genetically modified by baculoviral vectors. (United States)

    Zhao, Y; Lam, D H; Yang, J; Lin, J; Tham, C K; Ng, W H; Wang, S


    Tumor-tropic neural stem cells (NSCs) can be used in the Trojan horse approach as cellular vehicles for targeted delivery of therapeutic agents to distant tumor sites. To realize this cancer therapy potential, it is important to have a renewable source to generate large quantities of uniform human NSCs. Here, we reported that NSCs derived from HES1 human embryonic stem cell line were capable of migrating into intracranial glioma xenografts after systemic injection or after intracranial injection at a site distant from the tumor. To test whether the HES1-derived NSCs can be used for cancer gene therapy, we used a baculoviral vector to introduce the herpes simplex virus thymidine kinase suicide gene into the cells and demonstrated that baculovirus-mediated transgene expression may last for at least 3 weeks in NSCs. After being injected into the cerebral hemisphere opposite the tumor site and in the presence of ganciclovir, NSCs expressing the suicide gene were able to inhibit the growth of human glioma xenografts and prolong survival of tumor-bearing mice. Our findings suggest that human embryonic stem cells could potentially serve as a clinically viable source for production of cellular vehicles suitable for targeted anticancer gene therapy.

  18. Slow light vortices in periodic waveguides

    DEFF Research Database (Denmark)

    Sukhorukov, Andrey A.; Ha, Sangwoo; Desyatnikov, Anton S.


    We reveal that the reduction of the group velocity of light in periodic waveguides is generically associated with the presence of vortex energy flows. We show that the energy flows are gradually frozen for slow-light at the Brillouin zone edge, whereas vortices persist for slow-light states having...... non-vanishing phase velocity inside the Brillouin zone. We also demonstrate that presence of vortices can be linked to the absence of slow-light at the zone edge, and present calculations illustrating these general results....

  19. Water waves interacting with a current of constant vorticity: estimating the vorticity of the wave field (United States)

    Simon, Bruno; Seez, William; Abid, Malek; Kharif, Christian; Touboul, Julien


    During the last ten years, the topic of water waves interacting with sheared current has drawn a lot of attention, since the interaction of water waves with vorticity was recently found to be significant when modeling the propagation of water waves. In this framework, the configuration involving constantly sheared current (indeed a constant vorticity) is of special interst, since the equations remain tractable. In this framework, it is demonstrated that the flow related to water waves can be described by means of potential theory, since the source term in the vorticity equation is proportionnal to the curvature of the current profile (Nwogu, 2009). In the mean time, the community often wonders if this argument is valid, since the existence of a perfectly linearly sheared current is purely theoretical, and the presence of the vorticity within the wave field can be external (through wave generation mechanisms, for instance). Thus, this work is dedicated to investigate the magnitude of the vorticity related to the wave field, in conditions similar to this analytical case of constant vorticity. This approach is based on the comparison of experimental data, and three models. The first model is linear, supposing a constantly seared current and water waves described by potential theory. The second is fully nonlinear, but still supposing that water waves are potential, and finally, the third model is fully nonlinear, but solves the Euler equations, allowing the existence of vorticity related to the waves. The confrontation of these three approaches with the experimental data will allow to quantify the wave-related vorticity within the total flow, and analyze its importance as a function of nonlinearity and vorticity magnitude. ACKNOWLEDGEMENTS The DGA (Direction Générale de l'Armement, France) is acknowledged for its financial support through the ANR grant N°ANR-13-ASTR-0007.

  20. Cloning of fusion protein gene of Newcastle disease virus into a baculovirus derived bacmid shuttle vector, in order to express it in insect cell line

    Directory of Open Access Journals (Sweden)

    Hashemzadeh MS


    Full Text Available Abstract Background: Newcastle disease virus (NDV is one of the major pathogens in poultry and vaccination is intended to control the disease, as an effective solution, yet. Fusion protein (F on surface of NDV, has a fundamental role in virus pathogenicity and can induce protective immunity, alone. With this background, here our aim was to construct a baculovirus derived recombinant bacmid shuttle vector (encoding F-protein in order to express it in insect cell line. Materials and Methods: In this experimental study, at first complete F gene from avirulent strain La Sota of NDV was amplified by RT-PCR to produce F cDNA. The amplicon was cloned into T/A cloning vector and afterwards into pFastBac Dual donor plasmid. After the verification of cloning process by two methods, PCR and enzymatic digestion analysis, the accuracy of F gene sequence was confirmed by sequencing. Finally, F-containing recombinant bacmid was subsequently generated in DH10Bac cell and the construct production was confirmed by a special PCR panel, using F specific primers and M13 universal primers. Results: Analysis of confirmatory tests showed that the recombinant bacmid, expressing of F-protein gene in correct sequence and framework, has been constructed successfully. Conclusion: The product of this F-containing recombinant bacmid, in addition to its independent application in the induction of protective immunity, can be used with the other individual recombinant baculoviruses, expressing HN and NP genes to produce NDV-VLPs in insect cell line.

  1. Rescue and sprouting of motoneurons following ventral root avulsion and reimplantation combined with intraspinal adeno-associated viral vector-mediated expression of glial cell line-derived neurotrophic factor or brain-derived neurotrophic factor. (United States)

    Blits, Bas; Carlstedt, Thomas P; Ruitenberg, Marc Jan; de Winter, Fred; Hermens, Wim T J M C; Dijkhuizen, Paul A; Claasens, Jill W C; Eggers, Ruben; van der Sluis, Ronald; Tenenbaum, Liliane; Boer, Gerard J; Verhaagen, Joost


    Following avulsion of a spinal ventral root, motoneurons that project through the avulsed root are axotomized. Avulsion between, for example, L2 and L6 leads to denervation of hind limb muscles. Reimplantation of an avulsed root directed to the motoneuron pool resulted in re-ingrowth of some motor axons. However, most motoneurons display retrograde atrophy and subsequently die. Two neurotrophic factors, glial cell line-derived neurotrophic factor (GDNF) and brain-derived neurotrophic factor (BDNF), promote the survival of motoneurons after injury. The long-term delivery of these neurotrophic factors to the motoneurons in the ventral horn of the spinal cord is problematic. One strategy to improve the outcome of the neurosurgical reinsertion of the ventral root following avulsion would involve gene transfer with adeno-associated viral (AAV) vectors encoding these neurotrophic factors near the denervated motoneuron pool. Here, we show that AAV-mediated overexpression of GDNF and BDNF in the spinal cord persisted for at least 16 weeks. At both 1 and 4 months post-lesion AAV-BDNF- and -GDNF-treated animals showed an increased survival of motoneurons, the effect being more prominent at 1 month. AAV vector-mediated overexpression of neurotrophins also promoted the formation of a network of motoneuron fibers in the ventral horn at the avulsed side, but motoneurons failed to extent axons into the reinserted L4 root towards the sciatic nerve nor to improve functional recovery of the hind limbs. This suggests that high levels of neurotrophic factors in the ventral horn promote sprouting, but prevent directional growth of axons of a higher number of surviving motoneurons into the implanted root.

  2. {Γ} -Convergence Analysis of a Generalized XY Model: Fractional Vortices and String Defects (United States)

    Badal, Rufat; Cicalese, Marco; De Luca, Lucia; Ponsiglione, Marcello


    We propose and analyze a generalized two dimensional XY model, whose interaction potential has n weighted wells, describing corresponding symmetries of the system. As the lattice spacing vanishes, we derive by {Γ} -convergence the discrete-to-continuum limit of this model. In the energy regime we deal with, the asymptotic ground states exhibit fractional vortices, connected by string defects. The {Γ} -limit takes into account both contributions, through a renormalized energy, depending on the configuration of fractional vortices, and a surface energy, proportional to the length of the strings. Our model describes in a simple way several topological singularities arising in Physics and Materials Science. Among them, disclinations and string defects in liquid crystals, fractional vortices and domain walls in micromagnetics, partial dislocations and stacking faults in crystal plasticity.

  3. Observations of convection vortices in the afternoon sector using the SuperDARN HF radars

    Energy Technology Data Exchange (ETDEWEB)

    Bristow, W.A.; Greenwald, R.A.; Sibeck, D.G. [Johns Hopkins Univ., Laurel, MD (United States)] [and others


    Observations of convection vortices using the new SuperDARN HF radars are presented. The velocity field derived from the overlapping fields of view of the new HF radars at Kapuskasing, Ontario, and Saskatoon, Saskatchewan, Canada, often image the portion of the convection pattern near the convection reversal boundary. Observations from near the convection reversal boundary in the afternoon sector of October 22, 1993, showed two convection vortices evolving within the field of view of both radars. The first vortex appeared at about 2120 UT and the second at about 2145 UT: 1446 MLT and 1512 MLT, respectively. The vortices were roughly 900 km in diameter and moved tailward with a velocity of about 600 m/s. At the times the vortices were observed by the radars, ground-based magnetometers of the CANOPUS and MACCS chains show transient deflections of near 100 nT, and the GOES 6 and GOES 7 satellite magnetometers showed significant decreases in the magnetospheric magnetic field strength. Data from the Geotail satellite magnetometer lagged by an appropriate time interval indicated that there were southward turnings of the interplanetary magnetic field that coincided with the decreases of magnetospheric magnetic field strength. The observations differ in many respects from previously published vortex observations. It is theorized that the vortices were caused by the Kelvin-Helmholtz instability at the inner edge of the low-latitude boundary layer. 48 refs.

  4. Gravity–capillary waves in finite depth on flows of constant vorticity (United States)

    Hsu, Hung-Chu; Francius, Marc; Kharif, Christian


    This paper considers two-dimensional periodic gravity–capillary waves propagating steadily in finite depth on a linear shear current (constant vorticity). A perturbation series solution for steady periodic waves, accurate up to the third order, is derived using a classical Stokes expansion procedure, which allows us to include surface tension effects in the analysis of wave–current interactions in the presence of constant vorticity. The analytical results are then compared with numerical computations with the full equations. The main results are (i) the phase velocity is strongly dependent on the value of the vorticity; (ii) the singularities (Wilton singularities) in the Stokes expansion in powers of wave amplitude that correspond to a Bond number of 1/2 and 1/3, which are the consequences of the non-uniformity in the ordering of the Fourier coefficients, are found to be influenced by vorticity; (iii) different surface profiles of capillary–gravity waves are computed and the effect of vorticity on those profiles is shown to be important, in particular that the solutions exhibit type-2-like wave features, characterized by a secondary maximum on the surface profile with a trough between the two maxima. PMID:27956873

  5. Spontaneous Formation of Vector Vortex Beams in Vertical-Cavity Surface-Emitting Lasers with Feedback (United States)

    Jimenez-Garcia, Jesus; Rodriguez, Pedro; Guillet, T.; Ackemann, T.


    The spontaneous emergence of vector vortex beams with nonuniform polarization distribution is reported in a vertical-cavity surface-emitting laser (VCSEL) with frequency-selective feedback. Antivortices with a hyperbolic polarization structure and radially polarized vortices are demonstrated. They exist close to and partially coexist with vortices with uniform and nonuniform polarization distributions characterized by four domains of pairwise orthogonal polarization. The spontaneous formation of these nontrivial structures in a simple, nearly isotropic VCSEL system is remarkable and the vector vortices are argued to have solitonlike properties.

  6. Slow-light vortices in periodic waveguides

    DEFF Research Database (Denmark)

    Sukhorukov, Andrey A.; Ha, Sangwoo; Desyatnikov, Anton S.


    We reveal that the reduction of the group velocity of light in periodic waveguides is generically associated with the presence of vortex energy flows. We show that the energy flows are gradually frozen for slow-light at the Brillouin zone edge, whereas vortices persist for slow-light states havin...

  7. Vorticity dynamics in an intracranial aneurysm (United States)

    Le, Trung; Borazjani, Iman; Sotiropoulos, Fotis


    Direct Numerical Simulation is carried out to investigate the vortex dynamics of physiologic pulsatile flow in an intracranial aneurysm. The numerical solver is based on the CURVIB (curvilinear grid/immersed boundary method) approach developed by Ge and Sotiropoulos, J. Comp. Physics, 225 (2007) and is applied to simulate the blood flow in a grid with 8 million grid nodes. The aneurysm geometry is extracted from MRI images from common carotid artery (CCA) of a rabbit (courtesy Dr.Kallmes, Mayo Clinic). The simulation reveals the formation of a strong vortex ring at the proximal end during accelerated flow phase. The vortical structure advances toward the aneurysm dome forming a distinct inclined circular ring that connects with the proximal wall via two long streamwise vortical structures. During the reverse flow phase, the back flow results to the formation of another ring at the distal end that advances in the opposite direction toward the proximal end and interacts with the vortical structures that were created during the accelerated phase. The basic vortex formation mechanism is similar to that observed by Webster and Longmire (1998) for pulsed flow through inclined nozzles. The similarities between the two flows will be discussed and the vorticity dynamics of an aneurysm and inclined nozzle flows will be analyzed.This work was supported in part by the University of Minnesota Supercomputing Institute.

  8. Bilinear Relative Equilibria of Identical Point Vortices

    DEFF Research Database (Denmark)

    Aref, H.; Beelen, Peter; Brøns, Morten


    . In particular, we show that, given q(z)=z 2+η 2, where η is real, there is a unique p(z) of degree n, and a unique value of η 2=A n , such that the zeros of q(z) and p(z) form a relative equilibrium of n+2 point vortices. We show that $A_{n} \\approx\\frac{2}{3}n + \\frac{1}{2}$, as n→∞, where the coefficient of n......A new class of bilinear relative equilibria of identical point vortices in which the vortices are constrained to be on two perpendicular lines, conveniently taken to be the x- and y-axes of a Cartesian coordinate system, is introduced and studied. In the general problem we have m vortices on the y......, obtained using Sturm’s comparison theorem, is that if p(z) satisfies the ODE for a given q(z) with its imaginary zeros symmetric relative to the x-axis, then it must have at least n−m+2 simple, real zeros. For m=2 this provides a complete characterization of all zeros, and we study this case in some detail...

  9. Vortices in superconducting bulk, films and SQUIDs

    Indian Academy of Sciences (India)

    ... realistic shapes like thin and thick strips and disks or thin rectangular plates or films, containing pinned vortices, can be computed within continuum theory by solving an integral equation. A useful example is a thin square with a central hole and a radial slit, used as superconducting quantum interference device (SQUID).

  10. Vorticity Transport on a Rotating Blade (United States)

    Wojcik, Craig; Buchholz, James


    The development of the leading-edge vortex (LEV) is investigated on the suction surface of a rectangular flat plate undergoing a starting rotation in a quiescent fluid for angles of attack between 25 and 45 degrees. For blade aspect ratios of 2 and 4, the LEV is shown to be compact and quasi-stationary at inboard regions of the blade, consistent with the results of some other recent investigations. A salient feature of this flow is a region of opposite-sign vorticity generated on the blade beneath the LEV which is observed to become partially entrained into the LEV. A detailed vorticity transport analysis on the LEV has revealed that the resulting annihilation of vorticity is an important mechanism regulating LEV circulation, and therefore its stability. A parametric study is discussed, which elucidates the roles of shear layer vorticity flux, spanwise flow, vortex tilting, and annihilation on the evolution of LEV circulation with changes in azimuthal position, blade aspect ratio, spanwise position, and Reynolds number. This work was supported by the National Science Foundation (EPSCoR grant EPS1101284), the Air Force Office of Scientific Research (grant FA9550-11-1-0019), and IIHR - Hydroscience & Engineering.

  11. Vortices in theories with flat directions

    NARCIS (Netherlands)

    Achucarro, A; Davis, AC; Pickles, M; Urrestilla, J


    In theories with flat directions containing vortices, such as supersymmetric QED, there is a vacuum selection effect in the allowed asymptotic configurations. We explain the role played by gauge fields in this effect and give a simple criterion for determining what vacua will be chosen, namely,

  12. Numerical simulation of pump-intake vortices

    Directory of Open Access Journals (Sweden)

    Rudolf Pavel


    Full Text Available Pump pre-swirl or uneven flow distribution in front of the pump can induce pump-intake vortices. These phenomena result in blockage of the impeller suction space, deterioration of efficiency, drop of head curve and earlier onset of cavitation. Real problematic case, where head curve drop was documented, is simulated using commercial CFD software. Computational simulation was carried out for three flow rates, which correspond to three operating regimes of the vertical pump. The domain consists of the pump sump, pump itself excluding the impeller and the delivery pipe. One-phase approach is applied, because the vortex cores were not filled with air during observation of the real pump operation. Numerical simulation identified two surface vortices and one bottom vortex. Their position and strength depend on the pump flow rate. Paper presents detail analysis of the flow field on the pump intake, discusses influence of the vortices on pump operation and suggests possible actions that should be taken to suppress the intake vortices.

  13. Numerical simulation of pump-intake vortices (United States)

    Rudolf, Pavel; Klas, Roman


    Pump pre-swirl or uneven flow distribution in front of the pump can induce pump-intake vortices. These phenomena result in blockage of the impeller suction space, deterioration of efficiency, drop of head curve and earlier onset of cavitation. Real problematic case, where head curve drop was documented, is simulated using commercial CFD software. Computational simulation was carried out for three flow rates, which correspond to three operating regimes of the vertical pump. The domain consists of the pump sump, pump itself excluding the impeller and the delivery pipe. One-phase approach is applied, because the vortex cores were not filled with air during observation of the real pump operation. Numerical simulation identified two surface vortices and one bottom vortex. Their position and strength depend on the pump flow rate. Paper presents detail analysis of the flow field on the pump intake, discusses influence of the vortices on pump operation and suggests possible actions that should be taken to suppress the intake vortices.

  14. DensToolKit: A comprehensive open-source package for analyzing the electron density and its derivative scalar and vector fields (United States)

    Solano-Altamirano, J. M.; Hernández-Pérez, Julio M.


    DensToolKit is a suite of cross-platform, optionally parallelized, programs for analyzing the molecular electron density (ρ) and several fields derived from it. Scalar and vector fields, such as the gradient of the electron density (∇ρ), electron localization function (ELF) and its gradient, localized orbital locator (LOL), region of slow electrons (RoSE), reduced density gradient, localized electrons detector (LED), information entropy, molecular electrostatic potential, kinetic energy densities K and G, among others, can be evaluated on zero, one, two, and three dimensional grids. The suite includes a program for searching critical points and bond paths of the electron density, under the framework of Quantum Theory of Atoms in Molecules. DensToolKit also evaluates the momentum space electron density on spatial grids, and the reduced density matrix of order one along lines joining two arbitrary atoms of a molecule. The source code is distributed under the GNU-GPLv3 license, and we release the code with the intent of establishing an open-source collaborative project. The style of DensToolKit's code follows some of the guidelines of an object-oriented program. This allows us to supply the user with a simple manner for easily implement new scalar or vector fields, provided they are derived from any of the fields already implemented in the code. In this paper, we present some of the most salient features of the programs contained in the suite, some examples of how to run them, and the mathematical definitions of the implemented fields along with hints of how we optimized their evaluation. We benchmarked our suite against both a freely-available program and a commercial package. Speed-ups of ˜2×, and up to 12× were obtained using a non-parallel compilation of DensToolKit for the evaluation of fields. DensToolKit takes similar times for finding critical points, compared to a commercial package. Finally, we present some perspectives for the future development and

  15. Experimental Observations of Ion Phase-Space Vortices

    DEFF Research Database (Denmark)

    Pécseli, Hans; Armstrong, R. J.; Trulsen, J.


    Experimental observations of ion phase-space vortices are reported. The ion phase-space vortices form in the region of heated ions behind electrostatic ion acoustic shocks. The results are in qualitative agreement with numerical and analytic studies.......Experimental observations of ion phase-space vortices are reported. The ion phase-space vortices form in the region of heated ions behind electrostatic ion acoustic shocks. The results are in qualitative agreement with numerical and analytic studies....

  16. Cyclones and attractive streaming generated by acoustical vortices. (United States)

    Riaud, Antoine; Baudoin, Michael; Thomas, Jean-Louis; Bou Matar, Olivier


    Acoustical and optical vortices have attracted great interest due to their ability to capture and manipulate particles with the use of radiation pressure. Here we show that acoustical vortices can also induce axial vortical flow reminiscent of cyclones, whose topology can be controlled by adjusting the properties of the acoustical beam. In confined geometry, the phase singularity enables generating "attractive streaming" with the flow directed toward the transducer. This opens perspectives for contactless vortical flow control.

  17. About vectors

    CERN Document Server

    Hoffmann, Banesh


    From his unusual beginning in ""Defining a vector"" to his final comments on ""What then is a vector?"" author Banesh Hoffmann has written a book that is provocative and unconventional. In his emphasis on the unresolved issue of defining a vector, Hoffmann mixes pure and applied mathematics without using calculus. The result is a treatment that can serve as a supplement and corrective to textbooks, as well as collateral reading in all courses that deal with vectors. Major topics include vectors and the parallelogram law; algebraic notation and basic ideas; vector algebra; scalars and scalar p

  18. Vector analysis

    CERN Document Server

    Newell, Homer E


    When employed with skill and understanding, vector analysis can be a practical and powerful tool. This text develops the algebra and calculus of vectors in a manner useful to physicists and engineers. Numerous exercises (with answers) not only provide practice in manipulation but also help establish students' physical and geometric intuition in regard to vectors and vector concepts.Part I, the basic portion of the text, consists of a thorough treatment of vector algebra and the vector calculus. Part II presents the illustrative matter, demonstrating applications to kinematics, mechanics, and e

  19. Controlled Manipulation of Individual Vortices in a Superconductor

    Energy Technology Data Exchange (ETDEWEB)

    Straver, E.W.J.


    We report controlled local manipulation of single vortices by low temperature magnetic force microscope (MFM) in a thin film of superconducting Nb. We are able to position the vortices in arbitrary configurations and to measure the distribution of local depinning forces. This technique opens up new possibilities for the characterization and use of vortices in superconductors.

  20. High-titer foamy virus vector transduction and integration sites of human CD34+ cell–derived SCID-repopulating cells

    Directory of Open Access Journals (Sweden)

    Md Nasimuzzaman


    Full Text Available Foamy virus (FV vectors are promising tools for gene therapy, but low titer is a major challenge for large-scale clinical trials. Here, we increased FV vector titer 50-fold by constructing novel vector plasmids and using polyethylenimine-mediated transfection. FV and lentiviral (LV vectors were used separately to transduce human CD34+ cells at multiplicities of infection of 25, and those cells were transplanted into immunodeficient mice. FV vector transduction frequencies of repopulating human cells were 37.1 ± 1.9% in unstimulated cells and 36.9 ± 2.2% in prestimulated cells, and engraftment frequencies were 40.9 ± 4.9% in unstimulated cells and 47.1 ± 3.3% in prestimulated cells. Engraftment frequencies of FV vector-transduced cells were significantly higher than those of LV vector-transduced cells. Linear amplification-mediated PCR with Illumina paired-end runs showed that all human chromosomes contained FV provirus. FV had an integration preference near transcriptional start sites and CpG islands of RefSeq genes but not within genes. Repopulating lymphoid and myeloid cells contained common integration sites, suggesting that FV vector could transduce multilineage hematopoietic stem/progenitor populations. Our new FV vector backbone may be a suitable candidate for developing therapeutic FV vectors for use in clinical trials.

  1. An Electrostatically Self-Assembled Ternary Nanocomplex as a Non-Viral Vector for the Delivery of Plasmid DNA into Human Adipose-Derived Stem Cells

    Directory of Open Access Journals (Sweden)

    Sun-Hee Cho


    Full Text Available In this study, we developed electrostatically self-assembled ternary nanocomplexes as a safe and effective non-viral vector for the delivery of plasmid DNA (pDNA into human adipose-derived stem cells (hASCs. Although polyethylenimine (PEI polymers initially showed excellent performance as gene delivery carriers, their broad use has been limited by cytotoxicity resulting from their strong positive charge. To reduce the cytotoxicity, we utilized anionic hyaluronic acid (HA as a corona layer material for pDNA/PEI binary nanocomplexes. HA was also introduced to increase the targeting efficiency of pDNA/PEI nanocomplexes because HA has can bind CD44 that is highly expressed on the surface of hASCs. We confirmed that the addition of HA changed the surface charge of pDNA/PEI nanocomplexes from positive to negative. The pDNA/PEI/HA ternary nanocomplexes showed high transfection efficiency and low cytotoxicity compared with commercially available products. When hASCs were pretreated with HA to passivate CD44, the transfection efficiency of pDNA/PEI/HA nanocomplexes was significantly reduced. These results suggest that HA that can act as a targeting ligand to CD44 contributed to the improved transfection of pDNA into hASCs. Our novel pDNA/PEI/HA nanocomplexes may be used as an effective non-viral pDNA delivery system for hASCs.

  2. Neuroprotective effects of glial cell line-derived neurotrophic factor mediated by an adeno-associated virus vector in a transgenic animal model of amyotrophic lateral sclerosis. (United States)

    Wang, Li-Jun; Lu, Yan-Yan; Muramatsu, Shin-ichi; Ikeguchi, Kunihiko; Fujimoto, Ken-ichi; Okada, Takashi; Mizukami, Hiroaki; Matsushita, Takashi; Hanazono, Yutaka; Kume, Akihiro; Nagatsu, Toshiharu; Ozawa, Keiya; Nakano, Imaharu


    Amyotrophic lateral sclerosis (ALS) is a relentlessly progressive lethal disease that involves selective annihilation of motoneurons. Glial cell line-derived neurotrophic factor (GDNF) is proposed to be a promising therapeutic agent for ALS and other motor neuron diseases. Because adeno-associated virus (AAV) has been developed as an attractive gene delivery system with proven safety, we explored the therapeutic efficacy of intramuscular delivery of the GDNF gene mediated by an AAV vector (AAV-GDNF) in the G93A mouse model of ALS. We show here that AAV-GDNF leads to substantial and long-lasting expression of transgenic GDNF in a large number of myofibers with its accumulation at the sites of neuromuscular junctions. Detection of GDNF labeled with FLAG in the anterior horn neurons, but not beta-galactosidase expressed as a control, indicates that most of the transgenic GDNF observed there is retrogradely transported GDNF protein from the transduced muscles. This transgenic GDNF prevents motoneurons from their degeneration, preserves their axons innervating the muscle, and inhibits the treated-muscle atrophy. Furthermore, four-limb injection of AAV-GDNF postpones the disease onset, delays the progression of the motor dysfunction, and prolongs the life span in the treated ALS mice. Our finding thus indicates that AAV-mediated GDNF delivery to the muscle is a promising means of gene therapy for ALS.

  3. Bidirectional changes in water-maze learning following recombinant adenovirus-associated viral vector (rAAV)-mediated brain-derived neurotrophic factor expression in the rat hippocampus. (United States)

    Pietropaolo, Susanna; Paterna, Jean-Charles; Büeler, Hansruedi; Feldon, Joram; Yee, Benjamin K


    Alterations in hippocampal brain-derived neurotrophic factor (BDNF) expression have been implicated in the pathogenesis of emotional and cognitive dysfunction. Here, we induced BDNF overexpression in the rat hippocampus using recombinant adenovirus-associated viral (rAAV) vectors, and studied its long-term (2 months postinduction) effects on anxiety-related behaviour, exploration in the open field, and spatial learning in the water maze. Although the treatment successfully led to substantial elevation of hippocampal BDNF levels, its effect on spatial learning was bidirectional: a subset of rAAV-induced BDNF-overexpressing rats performed well above control level, whereas the rest were clearly impaired. This behavioural distinction corresponded to two markedly different levels of BDNF overexpression. The increase in dorsal hippocampal BDNF content achieved in the 'water-maze-impaired' subgroup was twice that attained in the 'water-maze-improved' rats. Although neither subgroup of rAAV-induced BDNF-overexpressing rats differed from controls in the open field, the 'water-maze-impaired' subgroup also showed a significant anxiolytic effect. Our results suggest that hippocampal BDNF elevation significantly affects cognitive and emotional behaviours, but the direction and magnitude of the effects critically depend on the precise levels of overexpression. This factor must be taken into account in future studies examining the functional consequences of hippocampal BDNF overexpression.

  4. Antisense RNA directed to the human papillomavirus type 16 E7 mRNA from herpes simplex virus type 1 derived vectors is expressed in CaSki cells and downregulates E7 mRNA. (United States)

    Kari, Ilkka; Syrjänen, Stina; Johansson, Bo; Peri, Piritta; He, Bin; Roizman, Bernard; Hukkanen, Veijo


    Human papillomavirus (HPV) infection is known to be the most important etiologic factor of cervical cancer. There is no HPV specific therapy available for treatment of invasive squamous cell carcinoma of the cervix and its precursor lesions. The present study elucidates the potential to use herpes simplex virus (HSV) derived vectors for expression of antisense RNA to HPV -16 E7 oncogene. We have constructed replication competent, nonneuroinvasive HSV-1 vectors, deleted of the gamma134.5 gene. The vectors express RNA antisense to the first 100 nucleotides of the HPV-16 E7 gene. We assayed the ability of the antisense E7 vectors R5225 (tk-) and R5226 (tk+), to produce antisense RNA, as well as the consequent effects on E7 mRNA and protein levels in HPV-16 positive CaSki cells. Anti-E7 RNA was expressed by both constructs in a dose-dependent manner. Expression of HPV-16 E7 mRNA was downregulated effectively in CaSki cells infected with the tk- recombinant R5225 or with R5226. The tk+ recombinant R5226 was effective in downregulating E7 protein expression. We have shown that anti-E7 RNA expressed from an HSV vector could efficiently downregulate HPV-16 E7 mRNA and E7 protein expression in CaSki cells. We conclude that HSV vectors may become a useful tool for gene therapy of HPV infections.

  5. Antisense RNA directed to the human papillomavirus type 16 E7 mRNA from herpes simplex virus type 1 derived vectors is expressed in CaSki cells and downregulates E7 mRNA

    Directory of Open Access Journals (Sweden)

    Johansson Bo


    Full Text Available Abstract Background Human papillomavirus (HPV infection is known to be the most important etiologic factor of cervical cancer. There is no HPV specific therapy available for treatment of invasive squamous cell carcinoma of the cervix and its precursor lesions. The present study elucidates the potential to use herpes simplex virus (HSV derived vectors for expression of antisense RNA to HPV -16 E7 oncogene. Results We have constructed replication competent, nonneuroinvasive HSV-1 vectors, deleted of the γ134.5 gene. The vectors express RNA antisense to the first 100 nucleotides of the HPV-16 E7 gene. We assayed the ability of the antisense E7 vectors R5225 (tk- and R5226 (tk+, to produce antisense RNA, as well as the consequent effects on E7 mRNA and protein levels in HPV-16 positive CaSki cells. Anti-E7 RNA was expressed by both constructs in a dose-dependent manner. Expression of HPV-16 E7 mRNA was downregulated effectively in CaSki cells infected with the tk- recombinant R5225 or with R5226. The tk+ recombinant R5226 was effective in downregulating E7 protein expression. Conclusion We have shown that anti-E7 RNA expressed from an HSV vector could efficiently downregulate HPV-16 E7 mRNA and E7 protein expression in CaSki cells. We conclude that HSV vectors may become a useful tool for gene therapy of HPV infections.

  6. Chiral heat wave and mixing of magnetic, vortical and heat waves in chiral media

    Energy Technology Data Exchange (ETDEWEB)

    Chernodub, M.N. [CNRS, Laboratoire de Mathématiques et Physique Théorique,Université de Tours, 37200 (France); Soft Matter Physics Laboratory, Far Eastern Federal University,Sukhanova 8, Vladivostok (Russian Federation); Department of Physics and Astronomy, University of Gent,Krijgslaan 281, S9, Gent (Belgium)


    We show that a hot rotating fluid of relativistic chiral fermions possesses a new gapless collective mode associated with coherent propagation of energy density and chiral density waves along the axis of rotation. This mode, which we call the Chiral Heat Wave, emerges due to a mixed gauge-gravitational anomaly. At finite density the Chiral Heat Wave couples to the Chiral Vortical Wave while in the presence of an external magnetic field it mixes with the Chiral Magnetic Wave. The coupling of the Chiral Magnetic and Chiral Vortical Waves is also demonstrated. We find that the coupled waves — which are coherent fluctuations of the vector, axial and energy currents — have generally different velocities compared to the velocities of the individual waves.


    Directory of Open Access Journals (Sweden)

    K. N. Volkov


    Full Text Available The paper deals with conceptions and methods for visual representation of research numerical results in the problems of fluid mechanics and gas. The three-dimensional nature of unsteady flow being simulated creates significant difficulties for the visual representation of results. It complicates control and understanding of numerical data, and exchange and processing of obtained information about the flow field. Approaches to vortical flows visualization with the usage of gradients of primary and secondary scalar and vector fields are discussed. An overview of visualization techniques for vortical flows using different definitions of the vortex and its identification criteria is given. Visualization examples for some solutions of gas dynamics problems related to calculations of jets and cavity flows are presented. Ideas of the vortical structure of the free non-isothermal jet and the formation of coherent vortex structures in the mixing layer are developed. Analysis of formation patterns for spatial flows inside large-scale vortical structures within the enclosed space of the cubic lid-driven cavity is performed. The singular points of the vortex flow in a cubic lid-driven cavity are found based on the results of numerical simulation; their type and location are identified depending on the Reynolds number. Calculations are performed with fine meshes and modern approaches to the simulation of vortical flows (direct numerical simulation and large-eddy simulation. Paradigm of graphical programming and COVISE virtual environment are used for the visual representation of computational results. Application that implements the visualization of the problem is represented as a network which links are modules and each of them is designed to solve a case-specific problem. Interaction between modules is carried out by the input and output ports (data receipt and data transfer giving the possibility to use various input and output devices.

  8. Elementary vectors

    CERN Document Server

    Wolstenholme, E Œ


    Elementary Vectors, Third Edition serves as an introductory course in vector analysis and is intended to present the theoretical and application aspects of vectors. The book covers topics that rigorously explain and provide definitions, principles, equations, and methods in vector analysis. Applications of vector methods to simple kinematical and dynamical problems; central forces and orbits; and solutions to geometrical problems are discussed as well. This edition of the text also provides an appendix, intended for students, which the author hopes to bridge the gap between theory and appl

  9. A fiber-modified adenoviral vector interacts with immunoevasion molecules of the B7 family at the surface of murine leukemia cells derived from dormant tumors

    Directory of Open Access Journals (Sweden)

    Rogée Sophie


    Full Text Available Abstract Tumor cells can escape the immune system by overexpressing molecules of the B7 family, e.g. B7-H1 (PD-L1 or CD86, which suppresses the anti-tumor T-cell responses through binding to the PD-1 receptor, and similarly for B7.1 (CD80, through binding to CTLA-4. Moreover, direct interactions between B7-H1 and B7.1 molecules are also likely to participate in the immunoevasion mechanism. In this study, we used a mouse model of tumor dormancy, DA1-3b leukemia cells. We previously showed that a minor population of DA1-3b cells persists in equilibrium with the immune system for long periods of time, and that the levels of surface expression of B7-H1 and B7.1 molecules correlates with the dormancy time. We found that leukemia cells DA1-3b/d365 cells, which derived from long-term dormant tumors and overexpressed B7-H1 and B7.1 molecules, were highly permissive to Ad5FB4, a human adenovirus serotype 5 (Ad5 vector pseudotyped with chimeric human-bovine fibers. Both B7-H1 and B7.1 were required for Ad5FB4-cell binding and entry, since (i siRNA silencing of one or the other B7 gene transcript resulted in a net decrease in the cell binding and Ad5FB4-mediated transduction of DA1-3b/d365; and (ii plasmid-directed expression of B7.1 and B7-H1 proteins conferred to Ad5FB4-refractory human cells a full permissiveness to this vector. Binding data and flow cytometry analysis suggested that B7.1 and B7-H1 molecules played different roles in Ad5FB4-mediated transduction of DA1-3b/d365, with B7.1 involved in cell attachment of Ad5FB4, and B7-H1 in Ad5FB4 internalization. BRET analysis showed that B7.1 and B7-H1 formed heterodimeric complexes at the cell surface, and that Ad5FB4 penton, the viral capsomere carrying the fiber projection, could negatively interfere with the formation of B7.1/B7-H1 heterodimers, or modify their conformation. As interactors of B7-H1/B7.1 molecules, Ad5FB4 particles and/or their penton capsomeres represent potential therapeutic agents

  10. Endogenous siRNAs and piRNAs derived from transposable elements and genes in the malaria vector mosquito Anopheles gambiae. (United States)

    Biryukova, Inna; Ye, Tao


    The siRNA and piRNA pathways have been shown in insects to be essential for regulation of gene expression and defence against exogenous and endogenous genetic elements (viruses and transposable elements). The vast majority of endogenous small RNAs produced by the siRNA and piRNA pathways originate from repetitive or transposable elements (TE). In D. melanogaster, TE-derived endogenous siRNAs and piRNAs are involved in genome surveillance and maintenance of genome integrity. In the medically relevant malaria mosquito Anopheles gambiae TEs constitute 12-16% of the genome size. Genetic variations induced by TE activities are known to shape the genome landscape and to alter the fitness in An. gambiae. Here, using bioinformatics approaches we analyzed the small RNA data sets from 6 libraries formally reported in a previous study and examined the expression of the mixed germline/somatic siRNAs and piRNAs produced in adult An. gambiae females. We characterized a large population of TE-derived endogenous siRNAs and piRNAs, which constitutes 56-60% of the total siRNA and piRNA reads in the analysed libraries. Moreover, we identified a number of protein coding genes producing gene-specific siRNAs and piRNAs that were generally expressed at much lower levels than the TE-associated small RNAs. Detailed sequence analysis revealed that An. gambiae piRNAs were produced by both "ping-pong" dependent (TE-associated piRNAs) and independent mechanisms (genic piRNAs). Similarly to D. melanogaster, more than 90% of the detected piRNAs were produced from TE-associated clusters in An. gambiae. We also found that biotic stress as blood feeding and infection with Plasmodium parasite, the etiological agent of malaria, modulated the expression levels of the endogenous siRNAs and piRNAs in An. gambiae. We identified a large and diverse set of the endogenously derived siRNAs and piRNAs that share common and distinct aspects of small RNA expression across insect species, and inferred their

  11. Intensity of vortices: from soap bubbles to hurricanes. (United States)

    Meuel, T; Xiong, Y L; Fischer, P; Bruneau, C H; Bessafi, M; Kellay, H


    By using a half soap bubble heated from below, we obtain large isolated single vortices whose properties as well as their intensity are measured under different conditions. By studying the effects of rotation of the bubble on the vortex properties, we found that rotation favors vortices near the pole. Rotation also inhibits long life time vortices. The velocity and vorticity profiles of the vortices obtained are well described by a Gaussian vortex. Besides, the intensity of these vortices can be followed over long time spans revealing periods of intensification accompanied by trochoidal motion of the vortex center, features which are reminiscent of the behavior of tropical cyclones. An analysis of this intensification period suggests a simple relation valid for both the vortices observed here and for tropical cyclones.

  12. Mimicking graphene with polaritonic spin vortices (United States)

    Gulevich, Dmitry R.; Yudin, Dmitry


    Exploring the properties of strongly correlated systems through quantum simulation with photons, cold atoms, or polaritons represents an active area of research. In fact, the latter sheds light on the behavior of complex systems that are difficult to address in the laboratory or to tackle numerically. In this study, we discuss an analog of graphene formed by exciton-polariton spin vortices arranged into a hexagonal lattice. We show how graphene-type dispersion at different energy scales arises for several types of exciton-polariton spin vortices. In contrast to previous studies of exciton polaritons in artificial lattices, the use of exciton-polariton spin vortex modes offers a richer playground for quantum simulations. In particular, we demonstrate that the sign of the nearest-neighbor coupling strength can be inverted.

  13. Trailing vortices from low speed flyers (United States)

    Waldman, Rye; Kudo, Jun; Breuer, Kenneth


    The structure and strength of the vortex wake behind a airplane or animal flying with a fixed or flapping wing contains valuable information about the aerodynamic load history. However, the amount of vorticity measured in the trailing vortex is not always in agreement with the known lift generated, and the behavior of these vortices at relatively low Reynolds numbers is also not well-understood. We present the results from a series of wind tunnel PIV experiments conducted behind a low-aspect ratio rectangular wing at a chord-Reynolds numbers of 30,000. In addition to wake PIV measurements measured in the cross-stream (Trefftz) plane, we measure the lift and drag directly using a six-axis force-torque transducer. We discuss how vortex size, shape, strength and position vary in time and downstream location, as well as the challenges associated with the use of PIV wake measurements to accurate determine aerodynamic forces.

  14. Statistical mechanics of vortices from field theory

    CERN Document Server

    Kajantie, Keijo; Neuhaus, T; Rajantie, A; Rummukainen, K


    We study with lattice Monte Carlo simulations the interactions and macroscopic behaviour of a large number of vortices in the 3-dimensional U(1) gauge+Higgs field theory, in an external magnetic field. We determine non-perturbatively the (attractive or repelling) interaction energy between two or more vortices, as well as the critical field strength H_c, the thermodynamical discontinuities, and the surface tension related to the boundary between the Meissner phase and the Coulomb phase in the type I region. We also investigate the emergence of vortex lattice and vortex liquid phases in the type II region. For the type I region the results obtained are in qualitative agreement with mean field theory, except for small values of H_c, while in the type II region there are significant discrepancies. These findings are relevant for superconductors and some models of cosmic strings, as well as for the electroweak phase transition in a magnetic field.

  15. Statistical mechanics of vortices from field theory (United States)

    Kajantie, K.; Laine, M.; Neuhaus, T.; Rajantie, A.; Rummukainen, K.


    We study with lattice Monte Carlo simulations the interactions and macroscopic behavior of a large number of vortices in the three-dimensional U(1) gauge + Higgs field theory, in an external magnetic field. We determine non-perturbatively the (attractive or repelling) interaction energy between two or more vortices, as well as the critical field strength Hc, the thermodynamical discontinuities, and the surface tension related to the boundary between the Meissner phase and the Coulomb phase in the type I region. We also investigate the emergence of vortex lattice and vortex liquid phases in the type II region. For the type I region the results obtained are in qualitative agreement with mean field theory, except for small values of Hc, while in the type II region there are significant discrepancies. These findings are relevant for superconductors and some models of cosmic strings, as well as for the electroweak phase transition in a magnetic field.

  16. Vortical and internal wave shear and strain


    Pinkel, R.


    Depth-time records of isopycnal vertical strain have been collected from intensive CTD profiling programs on the research platform (R/P) Floating Instrument Platform (FLIP). The associated vertical wavenumber frequency spectrum of strain, when viewed in an isopycnal-following frame, displays a clear spectral gap at low vertical wavenumber, separating the quasigeostrophic (vortical) strain field and the superinertial internal wave continuum. This gap enables both model and linear-filter-based ...

  17. Driven motion of vortices in superconductors

    Energy Technology Data Exchange (ETDEWEB)

    Crabtree, G.W.; Leaf, G.K.; Kaper, H.G.; Vinokur, V.M.; Koshelev, A.E.; Braun, D.W.; Levine, D.M.


    The driven motion of vortices in the solid vortex state is analyzed with the time-dependent Ginzburg-Landau equations. In large-scale numerical simulations, carried out on the IBM Scalable POWERparallel (SP) system at Argonne National Laboratory, many hundreds of vortices are followed as they move under the influence of a Lorentz force induced by a transport current in the presence of a planar defect (similar to a twin boundary in YBa{sub 2}CU{sub 3}O{sub 7}). Correlations in the positions and velocities of the vortices in plastic and elastic motion are identified and compared. Two types of plastic motion are observed. Organized plastic motion displaying long-range orientational correlation and shorter-range velocity correlation occurs when the driving forces are small compared to the pinning forces in the twin boundary. Disorganized plastic motion displaying no significant correlation in either the velocities or orientation of the vortex system occurs when the driving and pinning forces axe of the same order.

  18. Theoretical studies in mesoscale jets and vortices (United States)

    Radko, Timour


    Mesoscale vortices in the mid-ocean are known to move large distances without loss of coherence, preserving their speed and (usually westward) direction. Still open are the questions of how an eddy is able to preserve its structure during many turnaround times and what is the role in this process of the specific perturbations of the circular basic state. To investigate the effect of the rectilinear motion of the isolated eddies, we construct several analytical steady state models and examine the realizability in time of those solutions using the initial-value numerical calculations. To gain a preliminary understanding of the process, we first consider the barotropic f-plane model. It is demonstrated using linearized (about the circular basic state) calculations that for almost any eddy with compact basic velocity we can find a small amplitude disturbance of the first azimuthal harmonic (m=1 mode) that results in the rectilinear motion of an eddy. If such a disturbance is sufficiently small, the vortex can propagate many diameters away from its origin, as shown by a weak non-linear theory. This conclusion is confirmed by the spectral calculations using the full two dimensional vorticity equation. A more realistic representation of the ocean eddies is given by the equivalent-barotropic model, which includes effects of the passive lower layer and the ambient potential vorticity gradient (the beta-effect). Analytical theory is developed to construct a wide class of stable quasi-monopolar vortecies propagating in the westward direction with the supercritical (Usolutions in barotropic and equivalent-barotropic models for all values of the propagation velocity. The numerical spectral calculations, initiated by our analytical solutions, indicate that the (supercritical) vortices initially move with the predicted velocity, but later slow down to the speed of the long planetary waves. The period of time during which an eddy is propagating with its initial velocity is

  19. Vector analysis

    CERN Document Server

    Brand, Louis


    The use of vectors not only simplifies treatments of differential geometry, mechanics, hydrodynamics, and electrodynamics, but also makes mathematical and physical concepts more tangible and easy to grasp. This text for undergraduates was designed as a short introductory course to give students the tools of vector algebra and calculus, as well as a brief glimpse into these subjects' manifold applications. The applications are developed to the extent that the uses of the potential function, both scalar and vector, are fully illustrated. Moreover, the basic postulates of vector analysis are brou

  20. Instantons, vortices and confinement in SU(2) Yang-Mills theory

    Energy Technology Data Exchange (ETDEWEB)

    Lemos, A.L.L. de [Universidade Federal do Rio de Janeiro (UFRJ), RJ (Brazil); Oxman, L.E.; Teixeira, B.F.I. [Universidade Federal Fluminense (UFF), Niteroi, RJ (Brazil)


    Full text: In this work, we derive a recently proposed Abelian model to describe the interaction of correlated instantons, center vortices, and dual fields in three dimensional SU(2) Yang-Mills theory. Correlated monopoles and center vortices are believed to play a relevant role in accommodating the different properties of the confining string in Yang-Mills theories, receiving support from lattice simulations. In fact, scenarios based on either monopoles or closed center vortices are only partially successful to describe the expected behavior of the potential between quarks. At asymptotic distances, this potential should be linear and depend on the representation of the subgroup Z(N) of SU(N) (N-ality). At intermediate scales, it should posses Casimir scaling. The Cho-Faddeev- Niemi representation (CFN) can be used to associate monopoles with defects of the local color frame used to decompose the gauge fields. This possible frame defects can be extended to describe not only monopoles but also center vortices, correlated or not. In these scenarios, one of the difficulties is how to deal with the integration over an ensemble of extended objects, after considering a phenomenological parametrization of their properties, such as stiffness, interactions with dual fields, and interactions between them. This is particularly severe in four dimensional theories where center vortices generate two dimensional extended world surfaces. However, in three dimensions center vortices are stringlike and an ensemble of world lines is naturally associated with a second quantized field theory. The aim of this work is presenting a careful derivation of an effective model, considering instantons and center vortices in D=3 SU(3) theory, after parameterizing some intrinsic physical properties that these objects could present. One of the fundamental ingredients will be the adoption of recent techniques borrowed from polymer physics, where the extended objects are also one dimensional. This

  1. Weaving Knotted Vector Fields with Tunable Helicity (United States)

    Kedia, Hridesh; Foster, David; Dennis, Mark R.; Irvine, William T. M.


    We present a general construction of divergence-free knotted vector fields from complex scalar fields, whose closed field lines encode many kinds of knots and links, including torus knots, their cables, the figure-8 knot, and its generalizations. As finite-energy physical fields, they represent initial states for fields such as the magnetic field in a plasma, or the vorticity field in a fluid. We give a systematic procedure for calculating the vector potential, starting from complex scalar functions with knotted zero filaments, thus enabling an explicit computation of the helicity of these knotted fields. The construction can be used to generate isolated knotted flux tubes, filled by knots encoded in the lines of the vector field. Lastly, we give examples of manifestly knotted vector fields with vanishing helicity. Our results provide building blocks for analytical models and simulations alike.

  2. Weaving Knotted Vector Fields with Tunable Helicity. (United States)

    Kedia, Hridesh; Foster, David; Dennis, Mark R; Irvine, William T M


    We present a general construction of divergence-free knotted vector fields from complex scalar fields, whose closed field lines encode many kinds of knots and links, including torus knots, their cables, the figure-8 knot, and its generalizations. As finite-energy physical fields, they represent initial states for fields such as the magnetic field in a plasma, or the vorticity field in a fluid. We give a systematic procedure for calculating the vector potential, starting from complex scalar functions with knotted zero filaments, thus enabling an explicit computation of the helicity of these knotted fields. The construction can be used to generate isolated knotted flux tubes, filled by knots encoded in the lines of the vector field. Lastly, we give examples of manifestly knotted vector fields with vanishing helicity. Our results provide building blocks for analytical models and simulations alike.

  3. Visualization of vortical flows in computational fluid dynamics (United States)

    Volkov, K. N.; Emel'yanov, V. N.; Teterina, I. V.; Yakovchuk, M. S.


    The concepts and methods of the visual representation of fluid dynamics computations of vortical flows are studied. Approaches to the visualization of vortical flows based on the use of various definitions of a vortex and various tests for its identification are discussed. Examples of the visual representation of solutions to some fluid dynamics problems related to the computation of vortical flows in jets, channels, and cavities and of the computation of separated flows occurring in flows around bodies of various shapes are discussed.


    Directory of Open Access Journals (Sweden)

    T. O. Aubakirov


    Full Text Available A technique of calculation of aircraft condensation trails (contrails and wake vortices interaction is described. The technique is based on a suitable for real-time applications mathematical model of far wake utilizes the method of discrete vortices. The technique is supplemented by account of the influence of axial velocities in the vortex nucleus on contrail and wake vortex location. Results of calculations of contrails and wake vortices interaction for Il-76 and B-747 aircraft are presented.

  5. Phase shifting profilometry with optical vortices (United States)

    Sokolenko, B.; Poletaev, D.; Halilov, S.


    In this work we review principles and applications of a method of phase shifting profilometry with using of optical vortices imbedded into the probe beam. High spatial resolution caused by vortex phase sensitivity is analysable to retrieve the 2D and 3D shape of optically transparent and reflecting surfaces with exceeding of optical diffraction limit. This method applicable for non-destructive testing of thin films, live cells and biological tissues in real-time regime. Automatic processing of vortex interferograms with vortex phase shift analysis allow to achieve a vertical resolution down to 1,75 nm.

  6. Stability of relative equilibria of three vortices

    DEFF Research Database (Denmark)

    Aref, Hassan


    for the three eigenvalues determining the stability, including a new formula for the angular velocity of rotation of a collinear relative equilibrium. A graphical representation of the space of vortex circulations is introduced, and the resultants between various polynomials that enter the problem are used...... involved. The only comprehensive analysis available in the literature, by Tavantzis and Ting [Phys. Fluids 31, 1392 (1988)], is not easy to follow nor is it very physically intuitive. The symmetry between the three vortices is lost in this analysis. A different analysis is given based on explicit formulas...

  7. Observations of Electron Vorticity in the Inner Plasma Sheet (United States)

    Gurgiolo, C.; Goldstein, M. L.; Vinas, A. F.; Matthaeus, W. H.; Fazakerley, A. N.


    From a limited number of observations it appears that vorticity is a common feature in the inner plasma sheet. With the four Cluster spacecraft and the four PEACE instruments positioned in a tetrahedral configuration, for the first time it is possible to directly estimate the electron fluid vorticity in a space plasma. We show examples of electron fluid vorticity from multiple plasma sheet crossings. These include three time periods when Cluster passed through a reconnection ion diffusion region. Enhancements in vorticity are seen in association with each crossing of the ion diffusion region.

  8. Temporal evolution of vorticity staircases in randomly strained two-dimensional vortices (United States)

    Turner, M. R.


    The evolution of a Gaussian vortex subject to a weak-external-random n-fold multipolar strain field is examined using fully nonlinear simulations. The simulations show that at large Reynolds numbers, fine scale steps form at the periphery of the vortex, before merging, generally leaving one large step, which acts as a barrier between the vorticity within the coherent core and the surrounding, well mixed, "surf zone." It is shown for n = 2 that the width and the number of fine scale steps which initially form at the periphery of the vortex is dependent on the strain parameters, but that the range of radial values for which steps initially occur is only dependent on n and the amplitude of the strain field. A criteria is developed which can predict this range of radial values using the linear stability results of Le Dizès ["Non-axisymmetric vortices in two-dimensional flows," J. Fluid Mech. 406, 175 (2000)]. This criteria is based upon the perturbation vorticity needing to be larger than some fraction of the vorticity gradient to flatten the vortex profile. For n = 3 and 4, the radial step range is again predicted, and it is observed that for these higher wavenumbers the long lasting steps are narrower than the n = 2 case. For n = 4 the steps which form are so narrow that they do not persist very long before they are destroyed by the strain field and viscosity.

  9. Prediction of overall in vitro microsomal stability of drug candidates based on molecular modeling and support vector machines. Case study of novel arylpiperazines derivatives.

    Directory of Open Access Journals (Sweden)

    Szymon Ulenberg

    Full Text Available Other than efficacy of interaction with the molecular target, metabolic stability is the primary factor responsible for the failure or success of a compound in the drug development pipeline. The ideal drug candidate should be stable enough to reach its therapeutic site of action. Despite many recent excellent achievements in the field of computational methods supporting drug metabolism studies, a well-recognized procedure to model and predict metabolic stability quantitatively is still lacking. This study proposes a workflow for developing quantitative metabolic stability-structure relationships, taking a set of 30 arylpiperazine derivatives as an example. The metabolic stability of the compounds was assessed in in vitro incubations in the presence of human liver microsomes and NADPH and subsequently quantified by liquid chromatography-mass spectrometry (LC-MS. Density functional theory (DFT calculations were used to obtain 30 models of the molecules, and Dragon software served as a source of structure-based molecular descriptors. For modeling structure-metabolic stability relationships, Support Vector Machines (SVM, a non-linear machine learning technique, were found to be more effective than a regression technique, based on the validation parameters obtained. Moreover, for the first time, general sites of metabolism for arylpiperazines bearing the 4-aryl-2H-pyrido[1,2-c]pyrimidine-1,3-dione system were defined by analysis of Q-TOF-MS/MS spectra. The results indicated that the application of one of the most advanced chemometric techniques combined with a simple and quick in vitro procedure and LC-MS analysis provides a novel and valuable tool for predicting metabolic half-life values. Given the reduced time and simplicity of analysis, together with the accuracy of the predictions obtained, this is a valid approach for predicting metabolic stability using structural data. The approach presented provides a novel, comprehensive and reliable tool

  10. Adenovirus vector-mediated ex vivo gene transfer of brain-derived neurotrophic factor (BDNF) tohuman umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) promotescrush-injured rat sciatic nerve regeneration. (United States)

    Hei, Wei-Hong; Almansoori, Akram A; Sung, Mi-Ae; Ju, Kyung-Won; Seo, Nari; Lee, Sung-Ho; Kim, Bong-Ju; Kim, Soung-Min; Jahng, Jeong Won; He, Hong; Lee, Jong-Ho


    This study was designed toinvestigate the efficacy of adenovirus vector-mediated brain-derived neurotrophic factor (BDNF) ex vivo gene transfer to human umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) in a rat sciatic nerve crush injury model. BDNF protein and mRNA expression after infection was checked through an enzyme-linked immunosorbent assay (ELISA) and quantitative real-time polymerase chain reaction (qRT-PCR). Male Sprague-Dawley rats (200-250g, 6 weeks old) were distributed into threegroups (n=20 each): the control group, UCB-MSC group, and BDNF-adenovirus infected UCB-MSC (BDNF-Ad+UCB-MSC) group. UCB-MSCs (1×10 6 cells/10μl/rat) or BDNF-Ad+UCB-MSCs (1×10 6 cells/10μl/rat)were transplantedinto the rats at the crush site immediately after sciatic nerve injury. Cell tracking was done with PKH26-labeled UCB-MSCs and BDNF-Ad+UCB-MSCs (1×10 6 cells/10μl/rat). The rats were monitored for 4 weeks post-surgery. Results showed that expression of BDNF at both the protein and mRNA levels was higher inthe BDNF-Ad+UCB-MSC group compared to theUCB-MSC group in vitro.Moreover, BDNF mRNA expression was higher in both UCB-MSC group and BDNF-Ad+ UCB-MSC group compared tothe control group, and BDNF mRNA expression in theBDNF-Ad+UCB-MSC group was higher than inboth other groups 5days after surgeryin vivo. Labeled neurons in the dorsal root ganglia (DRG), axon counts, axon density, and sciatic function index were significantly increased in the UCB-MSC and BDNF-Ad+ UCB-MSCgroupscompared to the controlgroup four weeksaftercell transplantation. Importantly,the BDNF-Ad+UCB-MSCgroup exhibited more peripheral nerve regeneration than the other two groups.Our results indicate thatboth UCB-MSCs and BDNF-Ad+UCB-MSCscan improve rat sciatic nerve regeneration, with BDNF-Ad+UCB-MSCsshowing a greater effectthan UCB-MSCs. Copyright © 2017 Elsevier B.V. All rights reserved.

  11. Cloning vector (United States)

    Guilfoyle, R.A.; Smith, L.M.


    A vector comprising a filamentous phage sequence containing a first copy of filamentous phage gene X and other sequences necessary for the phage to propagate is disclosed. The vector also contains a second copy of filamentous phage gene X downstream from a promoter capable of promoting transcription in a bacterial host. In a preferred form of the present invention, the filamentous phage is M13 and the vector additionally includes a restriction endonuclease site located in such a manner as to substantially inactivate the second gene X when a DNA sequence is inserted into the restriction site. 2 figures.

  12. Cloning vector (United States)

    Guilfoyle, Richard A.; Smith, Lloyd M.


    A vector comprising a filamentous phage sequence containing a first copy of filamentous phage gene X and other sequences necessary for the phage to propagate is disclosed. The vector also contains a second copy of filamentous phage gene X downstream from a promoter capable of promoting transcription in a bacterial host. In a preferred form of the present invention, the filamentous phage is M13 and the vector additionally includes a restriction endonuclease site located in such a manner as to substantially inactivate the second gene X when a DNA sequence is inserted into the restriction site.

  13. Observed Effect of Mesoscale Vertical Vorticity on Rotation Sense of Dust Devil-Like Vortices in an Urban Area


    Fujiwara, Chusei; Yamashita, Kazuya; Fujiyoshi, Yasushi


    Dust devil-like vortices were detected by a three-dimensional scanning coherent Doppler lidar (3D-CDL) in an urban area of Sapporo, Japan, from April 2005 to July 2007. A total of 57 strong, dust devil-like vortices with vertical vorticity exceeding 0.1 s^[-1] were detected in 8 days of the observation period and were associated with a convective cell (fish net) pattern of wind fields detected by the 3D-CDL. The observed vortices had both rotation senses for 7 days. However, all of 7 dust dev...

  14. Vortices and magnetic structures of the target type in two-dimensional ferromagnetics with the anisotropic exchange interaction

    CERN Document Server

    Borisov, A B; Mikushina, N A; Moskvin, A S


    One investigated into structure of vortices and of other topological defects in two-dimensional Heisenberg ferromagnet. Paper contains basic data derived from microscopic theory of spin anisotropy in 3d- and 4f-element base systems. One studied effect of parameters of anisotropic exchange and single-ionic anisotropy on vortex structure. One studied magnetization vortices in freely plane ferromagnetic with anisotropic exchange and local anisotropy. One predicted and analyzed structure of a new class of static leading centre (target) type vortex configurations in anisotropic ferromagnet

  15. Melt pool vorticity in deep penetration laser material welding

    Indian Academy of Sciences (India)

    In the present study, the vorticity of melt motion in the keyhole and weld pool has been evaluated in case of high power CO2 laser beam welding. The circulation of vorticity is obtained as a function of Reynolds number for a given keyhole volume which is linked to Mach number variation. The shear stress and thermal fluxes ...

  16. Melt pool vorticity in deep penetration laser material welding

    Indian Academy of Sciences (India)

    Abstract. In the present study, the vorticity of melt motion in the keyhole and weld pool has been evaluated in case of high power CO2 laser beam welding. The circulation of vorticity is obtained as a function of Reynolds number for a given keyhole volume which is linked to Mach number variation. The shear stress and ther-.

  17. Diffused vorticity approach to the oscillations of a rotating Bose ...

    Indian Academy of Sciences (India)

    Abstract. The collective modes of a rotating Bose–Einstein condensate confined in an attractive quadratic plus quartic trap are investigated. Assuming the presence of a large number of vortices we apply the diffused vorticity approach to the system. We then use the sum rule technique for the calculation of collective ...

  18. Diffused vorticity approach to the oscillations of a rotating Bose ...

    Indian Academy of Sciences (India)

    The collective modes of a rotating Bose-Einstein condensate confined in an attractive quadratic plus quartic trap are investigated. Assuming the presence of a large number of vortices we apply the diffused vorticity approach to the system. We then use the sum rule technique for the calculation of collective frequencies, ...

  19. Direct observation of rectified motion of vortices by Lorentz microscopy

    Indian Academy of Sciences (India)

    Abstract. We have investigated the vortex dynamics for the 'ratchet' operation in a niobium superconductor via a direct imaging of Lorentz microscopy. We directly observe one-directional selective motion of field-gradient-driven vortices along fabricated channels. This results from the rectification of vortices in a spatially ...

  20. Direct observation of rectified motion of vortices by Lorentz microscopy

    Indian Academy of Sciences (India)

    We have investigated the vortex dynamics for the `ratchet' operation in a niobium superconductor via a direct imaging of Lorentz microscopy. We directly observe one-directional selective motion of field-gradient-driven vortices along fabricated channels. This results from the rectification of vortices in a spatially asymmetric ...

  1. The interplay between mosquitoes, entomopathogens and symbiotic microbes: A niche for the development of novel microbial-derived vector control strategies (United States)

    The current outbreak of Zika virus in the Americas has highlighted the need for improved methods of control. This concern is exacerbated if we consider that all three major arboviruses (Zika, dengue, and chikungunya virus) are transmitted efficiently by two wide spread mosquito vectors: Aedes aegypt...

  2. Potential vorticity field in the Bay of Bengal during southwest monsoon

    Digital Repository Service at National Institute of Oceanography (India)

    Murty, V.S.N.; Rao, D.P.

    theta), potential vorticity distribution is complex due to wind and freshwater forcings. The beta -effect dominates the potential vorticity field on 26.9 sigma theta isopycnal. The field of potential vorticity closely follows that of circulation...

  3. Helical vortices: linear stability analysis and nonlinear dynamics (United States)

    Selçuk, C.; Delbende, I.; Rossi, M.


    We numerically investigate, within the context of helical symmetry, the dynamics of a regular array of two or three helical vortices with or without a straight central hub vortex. The Navier–Stokes equations are linearised to study the instabilities of such basic states. For vortices with low pitches, an unstable mode is extracted which corresponds to a displacement mode and growth rates are found to compare well with results valid for an infinite row of point vortices or an infinite alley of vortex rings. For larger pitches, the system is stable with respect to helically symmetric perturbations. In the nonlinear regime, we follow the time-evolution of the above basic states when initially perturbed by the dominant instability mode. For two vortices, sequences of overtaking events, leapfrogging and eventually merging are observed. The transition between such behaviours occurs at a critical ratio involving the core size and the vortex-separation distance. Cases with three helical vortices are also presented.

  4. Decay of high order optical vortices in anisotropic nonlinear optical media

    DEFF Research Database (Denmark)

    Mamaev, A.V.; Saffman, M.; Zozulya, A.A.


    We present an experimental and theoretical study of the decay of high order optical vortices in media with an anisotropic nonlocal nonlinearity. Vortices with charge n decay into an aligned array of n vortices of unit charge.......We present an experimental and theoretical study of the decay of high order optical vortices in media with an anisotropic nonlocal nonlinearity. Vortices with charge n decay into an aligned array of n vortices of unit charge....

  5. Dipole vortices in the Great Australian Bight

    DEFF Research Database (Denmark)

    Cresswell, George R.; Lund-Hansen, Lars C.; Nielsen, Morten Holtegaard


    Shipboard measurements from late 2006 made by the Danish Galathea 3 Expedition and satellite sea surface temperature images revealed a chain of cool and warm mushroom' dipole vortices that mixed warm, salty, oxygen-poor waters on and near the continental shelf of the Great Australian Bight (GAB......) with cooler, fresher, oxygen-rich waters offshore. The alternating jets' flowing into the mushrooms were directed mainly northwards and southwards and differed in temperature by only 1.5 degrees C; however, the salinity difference was as much as 0.5, and therefore quite large. The GAB waters were slightly...... denser than the cooler offshore waters. The field of dipoles evolved and distorted, but appeared to drift westwards at 5km day-1 over two weeks, and one new mushroom carried GAB water southwards at 7km day(-1). Other features encountered between Cape Leeuwin and Tasmania included the Leeuwin Current...

  6. Potential vorticity patterns in Mediterranean "hurricanes" (United States)

    Miglietta, M. M.; Cerrai, D.; Laviola, S.; Cattani, E.; Levizzani, V.


    The potential vorticity (PV) anomalies due to the intrusion of dry stratospheric air and those generated by the tropospheric diabatic latent heating are qualitatively analyzed for five Mediterranean tropical-like cyclones (also known as Medicanes). Model simulations show the presence of an upper level PV streamer in the early stages of the cyclone, located on the left exit of a jet stream, and a middle-low level PV anomaly generated by the convection developing around the low-level vortex. In the mature stage, the upper level PV anomaly around the cyclone evolves differently for each case and appears somehow dependent on the lifetime. Only for the 2006 Medicane, the PV anomalies form an intense PV tower extending continuously from the lower troposphere to the lower stratosphere.

  7. Vortices and hysteresis in a rotating Bose-Einstein condensate with anharmonic confinement

    DEFF Research Database (Denmark)

    Jackson, A.D.; Kavoulakis, G.M.


    Vortices; Bose-Einstein condensation; phase diagrams; phase transformation Udgivelsesdato: 4 August......Vortices; Bose-Einstein condensation; phase diagrams; phase transformation Udgivelsesdato: 4 August...

  8. Vaccination to conserved influenza antigens in mice using a novel Simian adenovirus vector, PanAd3, derived from the bonobo Pan paniscus. (United States)

    Vitelli, Alessandra; Quirion, Mary R; Lo, Chia-Yun; Misplon, Julia A; Grabowska, Agnieszka K; Pierantoni, Angiolo; Ammendola, Virginia; Price, Graeme E; Soboleski, Mark R; Cortese, Riccardo; Colloca, Stefano; Nicosia, Alfredo; Epstein, Suzanne L


    Among approximately 1000 adenoviruses from chimpanzees and bonobos studied recently, the Pan Adenovirus type 3 (PanAd3, isolated from a bonobo, Pan paniscus) has one of the best profiles for a vaccine vector, combining potent transgene immunogenicity with minimal pre-existing immunity in the human population. In this study, we inserted into a replication defective PanAd3 a transgene expressing a fusion protein of conserved influenza antigens nucleoprotein (NP) and matrix 1 (M1). We then studied antibody and T cell responses as well as protection from challenge infection in a mouse model. A single intranasal administration of PanAd3-NPM1 vaccine induced strong antibody and T cell responses, and protected against high dose lethal influenza virus challenge. Thus PanAd3 is a promising candidate vector for vaccines, including universal influenza vaccines.

  9. Vaccination to conserved influenza antigens in mice using a novel Simian adenovirus vector, PanAd3, derived from the bonobo Pan paniscus.

    Directory of Open Access Journals (Sweden)

    Alessandra Vitelli

    Full Text Available Among approximately 1000 adenoviruses from chimpanzees and bonobos studied recently, the Pan Adenovirus type 3 (PanAd3, isolated from a bonobo, Pan paniscus has one of the best profiles for a vaccine vector, combining potent transgene immunogenicity with minimal pre-existing immunity in the human population. In this study, we inserted into a replication defective PanAd3 a transgene expressing a fusion protein of conserved influenza antigens nucleoprotein (NP and matrix 1 (M1. We then studied antibody and T cell responses as well as protection from challenge infection in a mouse model. A single intranasal administration of PanAd3-NPM1 vaccine induced strong antibody and T cell responses, and protected against high dose lethal influenza virus challenge. Thus PanAd3 is a promising candidate vector for vaccines, including universal influenza vaccines.

  10. Construction of a New Fusion Protein Vector Associated to Fibronectin Binding Protein A and Clumping Factor A derived from Staphylococcus aureus NCTC8325

    Directory of Open Access Journals (Sweden)

    Jamshid Faghri


    Full Text Available Objective(s Staphylococcus aureus is a leading cause of many nosocomial and community acquired infections. According to many reports, antibiotic therapy can not guarantee the eradication of S. aureus infections. Thus designing an adhesin based vaccine could restrain the S. aureus infections. This study designed for construction of a new fusion protein vaccine against S. aureus infections based on adhesin molecules fibronectin binding protein A (FnBPA and clumping factor A (ClfA. Materials and Methods Bioinformatic experiments were performed using Oligo analyzer and DNAMAN softwares. The fragments corresponding to fnbA binding domain and a C-terminal fragment from clfA were amplified from S. aureus NCTC8325 genomic DNA. Purified PCR products and the vector, pET15b, were digested with NcoI and BamHI. The digested PCR products were hybridized together and then ligated to digested vector. Finally incomplete construct was assembled by Taq DNA polymerase. To quick confirmation of cloning procedure the new construct designated pfnbA-clfA was digested with NcoI and BamHI. To further verification, the product was sent for sequencing. Results The data based on bioinformatic analysis showed no homology between fusion protein and human proteins. Digestion of new vector with NcoI and BamHI confirmed the ligation of fusion protein sequence into pET15b. Sequencing results verified the integrity of target sequences. Conclusion This study is the first effort to construct a new fusion protein vector based on S. aureus adhesins using a new design. This project is being continued to study the expression and biological activity of the fusion protein in a cell culture model.

  11. Complementation of a primer binding site-impaired murine leukemia virus-derived retroviral vector by a genetically engineered tRNA-like primer

    DEFF Research Database (Denmark)

    Lund, Anders Henrik; Duch, M; Lovmand, J


    , but not with a noncomplementary tRNA-like molecule. The engineered primer was shown to be involved in both the initiation of first-strand synthesis and second-strand transfer. These results provide an in vivo demonstration that the retroviral replication machinery may recognize sequence complementarity rather than actual primer...... binding site and 3' primer sequences. Use of mutated primer binding site vectors replicating via engineered primers may add additional control features to retroviral gene transfer technology....

  12. Solar Wind Simulations from the Sun to Earth Using a Data-Driven, MHD Model and Characteristically-Consistent Boundary Conditions Derived from SDO/HMI Vector Magnetograms (United States)

    Yalim, M. S.; Pogorelov, N.; Liu, Y.; Hu, Q.; Wu, S. T.


    The solar wind (SW) emerging from the Sun is the main driving mechanism of solar events which may lead to geomagnetic storms that are the primary causes of space weather disturbances that affect the magnetic environment of Earth and may have hazardous effects on the space-borne and ground-based technological systems as well as human health. Therefore, accurate modeling of the SW is very important to understand the underlying mechanisms of such storms. We have developed a data-driven, magnetohydrodynamic (MHD) model of the global solar corona which utilizes characteristic boundary conditions implemented within the Multi-Scale Fluid-Kinetic Simulation Suite (MS-FLUKSS) - a collection of problem oriented routines incorporated into the Chombo adaptive mesh refinement framework developed at Lawrence Berkeley National Laboratory. Our global solar corona model can be driven by both time-dependent and Carrington-rotation averaged vector magnetogram synoptic map data obtained by the Solar Dynamics Observatory/Helioseismic and Magnetic Imager (SDO/HMI) and the horizontal velocity data on the photosphere obtained by applying the Differential Affine Velocity Estimator for Vector Magnetograms method on the HMI-observed vector magnetic fields. In this study, we will present the results of three-dimensional global simulations of SW propagation from the Sun to Earth by using our global solar corona and inner heliosphere models and validate our results using spacecraft data at 1 AU.

  13. Vector geometry

    CERN Document Server

    Robinson, Gilbert de B


    This brief undergraduate-level text by a prominent Cambridge-educated mathematician explores the relationship between algebra and geometry. An elementary course in plane geometry is the sole requirement for Gilbert de B. Robinson's text, which is the result of several years of teaching and learning the most effective methods from discussions with students. Topics include lines and planes, determinants and linear equations, matrices, groups and linear transformations, and vectors and vector spaces. Additional subjects range from conics and quadrics to homogeneous coordinates and projective geom

  14. Calculus with vectors

    CERN Document Server

    Treiman, Jay S


    Calculus with Vectors grew out of a strong need for a beginning calculus textbook for undergraduates who intend to pursue careers in STEM. fields. The approach introduces vector-valued functions from the start, emphasizing the connections between one-variable and multi-variable calculus. The text includes early vectors and early transcendentals and includes a rigorous but informal approach to vectors. Examples and focused applications are well presented along with an abundance of motivating exercises. All three-dimensional graphs have rotatable versions included as extra source materials and may be freely downloaded and manipulated with Maple Player; a free Maple Player App is available for the iPad on iTunes. The approaches taken to topics such as the derivation of the derivatives of sine and cosine, the approach to limits, and the use of "tables" of integration have been modified from the standards seen in other textbooks in order to maximize the ease with which students may comprehend the material. Additio...

  15. Heat transfer enhancement using tip and junction vortices (United States)

    Gentry, Mark Cecil


    Single-phase convective heat transfer can be enhanced by modifying the heat transfer surface to passively generate streamwise vortices. The swirling flow of the vortices modifies the temperature field, thinning the thermal boundary layer and increasing surface convection. Tip vortices generated by delta wings and junction vortices generated by hemispherical protuberances were studied in laminar flat-plate and developing channel flows. Local and average convective measurements were obtained, and the structure of the vortices was studied using quantitative flow visualization and vortex strength measurements. The pressure drop penalty associated with the heat transfer enhancement was also investigated. Tip vortices generated by delta wings enhanced local convection by as much as 300% over a flat-plate boundary layer flow. Vortex strength increased with Reynolds number based on chord length, wing aspect ratio, and wing angle of attack. As the vortices were advected downstream, they decayed because of viscous interactions. In the developing channel flow, tip vortices produced a significant local heat transfer enhancement on both sides of the channel. The largest spatially averaged heat transfer enhancement was 55%; it was accompanied by a 100% increase in the pressure drop relative to the same channel flow with no delta-wing vortex generator. Junction vortices created by hemispherical surface protuberances provided local heat transfer enhancements as large as 250%. Vortex strength increased with an increasing ratio of hemisphere radius to local boundary layer thickness on a flat plate. In the developing channel flows, heat transfer enhancements were observed on both sides of the channel. The largest spatially averaged heat transfer enhancement was 50%; it was accompanied by a 90% pressure drop penalty relative to the same channel flow with no hemispherical vortex generator. This research is important in compact heat exchanger design. Enhancing heat transfer can lead to


    NARCIS (Netherlands)

    Thomas, E. G. F.


    This paper deals with the theory of integration of scalar functions with respect to a measure with values in a, not necessarily locally convex, topological vector space. It focuses on the extension of such integrals from bounded measurable functions to the class of integrable functions, proving

  17. Thermal Convection and Emergence of Isolated Vortices in Soap Bubbles (United States)

    Seychelles, F.; Amarouchene, Y.; Bessafi, M.; Kellay, H.


    A novel thermal convection cell consisting of half a soap bubble heated at the equator is introduced to study thermal convection and the movement of isolated vortices. The soap bubble, subject to stratification, develops thermal convection at its equator. A particular feature of this cell is the emergence of isolated vortices. These vortices resemble hurricanes or cyclones and similarities between our observed structures and these natural objects are found. This is brought forth through a study of the mean square displacement of these objects showing signs of superdiffusion.

  18. Thermal convection and emergence of isolated vortices in soap bubbles. (United States)

    Seychelles, F; Amarouchene, Y; Bessafi, M; Kellay, H


    A novel thermal convection cell consisting of half a soap bubble heated at the equator is introduced to study thermal convection and the movement of isolated vortices. The soap bubble, subject to stratification, develops thermal convection at its equator. A particular feature of this cell is the emergence of isolated vortices. These vortices resemble hurricanes or cyclones and similarities between our observed structures and these natural objects are found. This is brought forth through a study of the mean square displacement of these objects showing signs of superdiffusion.

  19. Synthesis and analysis of linear and nonlinear acoustical vortices. (United States)

    Marchiano, Régis; Thomas, Jean-Louis


    Acoustical screw dislocations are synthesized in various configurations with a versatile experimental setup. The experimental setup is based on the inverse filter technique and allows one to synthesize one or more acoustical vortices with a chosen width, position, and topological charge. An interesting feature of this experimental facility to study screw dislocation behavior is the direct measurement in amplitude and phase. This characteristic is used to develop an original method of decomposition of an acoustical vortex field in order to analyze the acoustical vortices. Moreover, the behaviors of two acoustical vortices of the same or opposite charge have been studied experimentally and compared to theoretical laws.

  20. Experimental Study of the Interaction between Vortices in Electrolytic Solutions (United States)

    Izaguirre, E. W.


    The interaction between vortices and their structure are analyzed in a system of three vortices in electrolytic solutions using crosed electric and magnetic fields in a square tray. The flow pattern and the speed field are obtained using image techniques and anemometry. The experiment is repeated for different strengths of the magnetic and electric fields, and thickness of the fluid layer. Self oscillations, coupling and competition between vortices are observed. A model of the system is presented and numerical results contrasted with the experimental data.

  1. Unsteady flow phenomena associated with leading-edge vortices (United States)

    Breitsamter, C.


    This paper presents selected results from extensive experimental investigations on turbulent flow fields and unsteady surface pressures caused by leading-edge vortices, in particular, for vortex breakdown flow. Such turbulent flows may cause severe dynamic aeroelastic problems like wing and/or fin buffeting on fighter-type aircraft. The wind tunnel models used include a generic delta wing as well as a detailed aircraft configuration of canard-delta wing type. The turbulent flow structures are analyzed by root-mean-square and spectral distributions of velocity and pressure fluctuations. Downstream of bursting local maxima of velocity fluctuations occur in a limited radial range around the vortex center. The corresponding spectra exhibit significant peaks indicating that turbulent kinetic energy is channeled into a narrow band. These quasi-periodic velocity oscillations arise from a helical mode instability of the breakdown flow. Due to vortex bursting there is a characteristic increase in surface pressure fluctuations with increasing angle of attack, especially when the burst location moves closer to the apex. The pressure fluctuations also show dominant frequencies corresponding to those of the velocity fluctuations. Using the measured flow field data, scaling parameters are derived for design purposes. It is shown that a frequency parameter based on the local semi-span and the sinus of angle of attack can be used to estimate the frequencies of dynamic loads evoked by vortex bursting.

  2. Vector Flow Visualization of Urinary Flow Dynamics in a Bladder Outlet Obstruction Model. (United States)

    Ishii, Takuro; Yiu, Billy Y S; Yu, Alfred C H


    Voiding dysfunction that results from bladder outlet (BO) obstruction is known to alter significantly the dynamics of urine passage through the urinary tract. To non-invasively image this phenomenon on a time-resolved basis, we pursued the first application of a recently developed flow visualization technique called vector projectile imaging (VPI) that can track the spatiotemporal dynamics of flow vector fields at a frame rate of 10,000 fps (based on plane wave excitation and least-squares Doppler vector estimation principles). For this investigation, we designed a new anthropomorphic urethral tract phantom to reconstruct urinary flow dynamics under controlled conditions (300 mm H2O inlet pressure and atmospheric outlet pressure). Both a normal model and a diseased model with BO obstruction were developed for experimentation. VPI cine loops were derived from these urinary flow phantoms. Results show that VPI is capable of depicting differences in the flow dynamics of normal and diseased urinary tracts. In the case with BO obstruction, VPI depicted the presence of BO flow jet and vortices in the prostatic urethra. The corresponding spatial-maximum flow velocity magnitude was estimated to be 2.43 m/s, and it is significantly faster than that for the normal model (1.52 m/s) and is in line with values derived from computational fluid dynamics simulations. Overall, this investigation demonstrates the feasibility of using vector flow visualization techniques to non-invasively examine internal flow characteristics related to voiding dysfunction in the urethral tract. Copyright © 2017 World Federation for Ultrasound in Medicine & Biology. Published by Elsevier Inc. All rights reserved.

  3. Toward lattice fractional vector calculus (United States)

    Tarasov, Vasily E.


    An analog of fractional vector calculus for physical lattice models is suggested. We use an approach based on the models of three-dimensional lattices with long-range inter-particle interactions. The lattice analogs of fractional partial derivatives are represented by kernels of lattice long-range interactions, where the Fourier series transformations of these kernels have a power-law form with respect to wave vector components. In the continuum limit, these lattice partial derivatives give derivatives of non-integer order with respect to coordinates. In the three-dimensional description of the non-local continuum, the fractional differential operators have the form of fractional partial derivatives of the Riesz type. As examples of the applications of the suggested lattice fractional vector calculus, we give lattice models with long-range interactions for the fractional Maxwell equations of non-local continuous media and for the fractional generalization of the Mindlin and Aifantis continuum models of gradient elasticity.

  4. Sparsified-dynamics modeling of discrete point vortices with graph theory (United States)

    Taira, Kunihiko; Nair, Aditya


    We utilize graph theory to derive a sparsified interaction-based model that captures unsteady point vortex dynamics. The present model builds upon the Biot-Savart law and keeps the number of vortices (graph nodes) intact and reduces the number of inter-vortex interactions (graph edges). We achieve this reduction in vortex interactions by spectral sparsification of graphs. This approach drastically reduces the computational cost to predict the dynamical behavior, sharing characteristics of reduced-order models. Sparse vortex dynamics are illustrated through an example of point vortex clusters interacting amongst themselves. We track the centroids of the individual vortex clusters to evaluate the error in bulk motion of the point vortices in the sparsified setup. To further improve the accuracy in predicting the nonlinear behavior of the vortices, resparsification strategies are employed for the sparsified interaction-based models. The model retains the nonlinearity of the interaction and also conserves the invariants of discrete vortex dynamics; namely the Hamiltonian, linear impulse, and angular impulse as well as circulation. Work supported by US Army Research Office (W911NF-14-1-0386) and US Air Force Office of Scientific Research (YIP: FA9550-13-1-0183).

  5. An introduction to vectors, vector operators and vector analysis

    CERN Document Server

    Joag, Pramod S


    Ideal for undergraduate and graduate students of science and engineering, this book covers fundamental concepts of vectors and their applications in a single volume. The first unit deals with basic formulation, both conceptual and theoretical. It discusses applications of algebraic operations, Levi-Civita notation, and curvilinear coordinate systems like spherical polar and parabolic systems and structures, and analytical geometry of curves and surfaces. The second unit delves into the algebra of operators and their types and also explains the equivalence between the algebra of vector operators and the algebra of matrices. Formulation of eigen vectors and eigen values of a linear vector operator are elaborated using vector algebra. The third unit deals with vector analysis, discussing vector valued functions of a scalar variable and functions of vector argument (both scalar valued and vector valued), thus covering both the scalar vector fields and vector integration.

  6. Equivariant Verlinde Formula from Fivebranes and Vortices (United States)

    Gukov, Sergei; Pei, Du


    We study complex Chern-Simons theory on a Seifert manifold M 3 by embedding it into string theory. We show that complex Chern-Simons theory on M 3 is equivalent to a topologically twisted supersymmetric theory and its partition function can be naturally regularized by turning on a mass parameter. We find that the dimensional reduction of this theory to 2d gives the low energy dynamics of vortices in four-dimensional gauge theory, the fact apparently overlooked in the vortex literature. We also generalize the relations between (1) the Verlinde algebra, (2) quantum cohomology of the Grassmannian, (3) Chern-Simons theory on {Σ× S^1} and (4) index of a spin c Dirac operator on the moduli space of flat connections to a new set of relations between (1) the "equivariant Verlinde algebra" for a complex group, (2) the equivariant quantum K-theory of the vortex moduli space, (3) complex Chern-Simons theory on {Σ × S^1} and (4) the equivariant index of a spin c Dirac operator on the moduli space of Higgs bundles.

  7. Artificial ice using superconducting vortices (Conference Presentation) (United States)

    Trastoy Quintela, Juan; Malnou, Maxime; Ulysse, Christian; Bernard, Rozenn; Bergeal, Nicolas; Faini, Giancarlo; Lesueur, Jerome; Briatico, Javier; Villegas, Javier E.


    We use magnetic flux quanta (superconducting vortices) on artificial energy landscapes (pinning arrays) to create a new type of artificial ice. This vortex ice shows unusual temperature effects that offer new possibilities in the study of ice systems. We have investigated the matching of the flux lattice to pinning arrays that present geometrical frustration. The pinning arrays are fabricated on YBCO films using masked O+ ion irradiation. The details of the magneto-resistance imply that the flux lattice organizes into a vortex ice. The absence of history-dependent effects suggests that the vortex ice is highly ordered. Due to the technique used for the artificial energy landscape fabrication, we have the ability to change the pinning array geometry using temperature as a control knob. In particular we can switch the geometrical frustration on and off, which opens the door to performing a new type of annealing absent in other artificial ice systems. * Work supported by the French ANR "MASTHER", and the Fundación Barrié (Galicia, Spain)

  8. Analytical BPS Maxwell-Higgs Vortices

    Directory of Open Access Journals (Sweden)

    R. Casana


    Full Text Available We have established a prescription for the calculation of analytical vortex solutions in the context of generalized Maxwell-Higgs models whose overall dynamics is controlled by two positive functions of the scalar field, namely, fϕ and wϕ. We have also determined a natural constraint between these functions and the Higgs potential Uϕ, allowing the existence of axially symmetric Bogomol'nyi-Prasad-Sommerfield (BPS solutions possessing finite energy. Furthermore, when the generalizing functions are chosen suitably, the nonstandard BPS equations can be solved exactly. We have studied some examples, comparing them with the usual Abrikosov-Nielsen-Olesen (ANO solution. The overall conclusion is that the analytical self-dual vortices are well-behaved in all relevant sectors, strongly supporting the consistency of the respective generalized models. In particular, our results mimic well-known properties of the usual (numerical configurations, as localized energy density, while contributing to the understanding of topological solitons and their description by means of analytical methods.


    Energy Technology Data Exchange (ETDEWEB)

    Fu, Wen; Liang, Edison [Department of Physics and Astronomy, Rice University, Houston, TX 77005 (United States); Li, Hui; Li, Shengtai [Los Alamos National Laboratory, Los Alamos, NM 87545 (United States); Lubow, Stephen, E-mail: [Space Telescope Science Institute, Baltimore, MD 21218 (United States)


    We carried out two-dimensional, high-resolution simulations to study the effect of dust feedback on the evolution of vortices induced by massive planets in protoplanetary disks. Various initial dust to gas disk surface density ratios (0.001-0.01) and dust particle sizes (Stokes number 4 × 10{sup –4}-0.16) are considered. We found that while dust particles migrate inward, vortices are very effective at collecting them. When dust density becomes comparable to gas density within the vortex, a dynamical instability is excited and it alters the coherent vorticity pattern and destroys the vortex. This dust feedback effect is stronger with a higher initial dust/gas density ratio and larger dust grain. Consequently, we found that the disk vortex lifetime can be reduced up to a factor of 10. We discuss the implications of our findings on the survivability of vortices in protoplanetary disks and planet formation.

  10. The decay of longitudinal vortices shed from airfoil vortex generators (United States)

    Wendt, Bruce J.; Reichert, Bruce A.; Foster, Jeffry D.


    An experimental study is conducted to examine the crossplane structure and streamwise decay of vortices shed from airfoil-type vortex generators. The vortex generators are set in a counter-rotating array spanning the full circumference of a straight pipe. The span of the vortex generators above the duct surface, h, is approximately equal to the local turbulent boundary layer thickness, delta. Measurement of three-component mean flow velocity in downstream crossplanes are used to characterize the structure of the shed vortices. Measurements in adjacent crossplanes (closely spaced along the streamwise coordinate) characterize the interaction and decay of the embedded vortices. A model constructed by the superposition of Oseen vortices is compared to the data for one test case.

  11. A numerical study of vorticity-enhanced heat transfer (United States)

    Wang, Xiaolin; Alben, Silas


    The Glezer lab at Georgia Tech has found that vorticity produced by vibrated reeds can improve heat transfer in electronic hardware. Vortices enhance forced convection by boundary layer separation and thermal mixing in the bulk flow. In this work, we simulate the heat transfer process in a 3-dimensional plate-fin heat sink. We propose a simplified model by considering flow and temperature in a 2-D channel, and extend the model to the third dimension using a 1-D heat fin model. We simulate periodically steady-state solutions. We determine how the global Nusselt number is increased, depending on the vortices' strengths and spacings, in the parameter space of Reynolds and Peclet numbers. We find a surprising spatial oscillation of the local Nusselt number due to the vortices. Support from NSF-DMS grant 1022619 is acknowledged.

  12. Image simulations of kinked vortices for transmission electron microscopy

    DEFF Research Database (Denmark)

    Beleggia, Marco; Pozzi, G.; Tonomura, A.


    We present an improved model of kinked vortices in high-Tc superconductors suitable for the interpretation of Fresnel or holographic observations carried out with a transmission electron microscope. A kinked vortex is composed of two displaced half-vortices, perpendicular to the film plane......, connected by a horizontal flux-line in the plane, resembling a connecting Josephson vortex (JV) segment. Such structures may arise when a magnetic field is applied almost in the plane, and the line tension of the fluxon breaks down under its influence. The existence of kinked vortices was hinted in earlier...... observations of high-Tc superconducting films, where the Fresnel contrast associated with some vortices showed a dumbbell like appearance. Here, we show that under suitable conditions the JV segment may reveal itself in Fresnel imaging or holographic phase mapping in a transmission electron microscope....

  13. A Generalized Form of Lait's Modified Potential Vorticity. (United States)

    Müller, Rolf; Günther, Gebhard


    Ertel's potential vorticity P is in widespread use as a diagnostic of dynamical processes in the stratosphere. For a variety of applications, however, the exponential increase of P with altitude is problematic. For this reason, Lait proposed a modified potential vorticity L, where a physically meaningful scaling is introduced that removes much of the altitude dependence of P. Here a generalized form of L is proposed by introducing an additional degree of freedom in the scaling. This generalized modified potential vorticity g possesses the same conservation properties as L itself and as the classic potential vorticity P but can be adjusted more closely to the specific situation under investigation. Comparison, over a large altitude range in the stratosphere, of fields of g with dynamical measures of the polar vortex edge and with observations of the long-lived trace gas N2O shows that g constitutes a more intuitively interpretable quantity than L.

  14. PIV and Hotwire Measurement and Analysis of Tip Vortices and Turbulent Wake Generated by a Model Horizontal Axis Wind Turbine (United States)

    Green, D.; Tan, Y. M.; Chamorro, L. P.; Arndt, R.; Sotiropoulos, F.; Sheng, J.


    Understanding vortical flow structures and turbulence in the wake flow behind a Horizontal Axis Wind Turbine (HAWT) has widespread applications in efficient blade design. Moreover, the knowledge of wake-turbine interactions allows us to devise optimal operational parameters, such as the spatial allocation and control algorithms of wind turbines, for a densely populated wind farm. To understand the influence of tip vortices on energy containing mean flow and turbulence, characteristics of vortical structures and turbulence must be quantified thoroughly. In this study, we conduct phase-locked Particle Image Velocimetry (PIV) measurements of the flow before and after a model HAWT, which is located in a zero-pressure gradient wind tunnel with a cross section of 1.7 × 1.7 m and a test section of 16 m in length. A three-blade model HAWT with a diameter of 605 mm and tip-speed ratio of 5 is used. PIV images are recorded by a 2048 × 2048 CCD camera and streamed at 6 Hz continuously; and phased locked with the passage of the blade at its vertical position. Each PIV measurement covers a 0.13 × 0.13 m2 sample area with the spatial resolution of 63 μm and a vector spacing of 0.5 mm. All experiments are conducted at the free-stream wind speed of 10 m/s. Flow fields at thirty consecutive downstream locations up to six rotor diameters and 144 mid chord lengths are measured. At each location, we obtain at least 10,000 instantaneous PIV realizations or 20,000 images. Three different configurations: single, dual, and trio turbines located at 5 rotor diameter upstream to each other, are examined experimentally. The flow statistics include mean wake velocity distributions, characteristics of tip vortices evolving downstream, fluctuation velocity, turbulent kinetic energy, stresses, and energy spectra. We find that tip vortices decay much faster in the wake of the upstream turbines (multiple-turbine configurations), whereas they maintain the coherence and strength behind a single

  15. Vector velocimeter

    DEFF Research Database (Denmark)


    for generation of a reference beam, a detector system comprising a first detector arrangement arranged in such a way that the signal beam and the reference beam are incident upon the first detector arrangement with the reference beam propagating at an angle relative to a signal beam, and wherein the first......The present invention relates to a compact, reliable and low-cost vector velocimeter for example for determining velocities of particles suspended in a gas or fluid flow, or for determining velocity, displacement, rotation, or vibration of a solid surface, the vector velocimeter comprising a laser...... assembly for emission of a measurement beam for illumination of an object in a measurement volume with coherent light whereby a signal beam emanating from the object in the measurement volume is formed in response to illumination of the object by the measurement beam, a reference beam generator...

  16. Fractional vortices in the XY model with $\\pi$ bonds


    Kulkarni, R. V.; Almaas, E.; Fisher, K. D.; Stroud, D.


    We define a new set of excitations in the XY model which we call ``fractional vortices''. In the frustrated XY model containing $\\pi$ bonds, we make the ansatz that the ground state configurations can be characterized by pairs of oppositely charged fractional vortices. For a chain of $\\pi$ bonds, the ground state energy and the phase configurations calculated on the basis of this ansatz agree well with the results from direct numerical simulations. Finally, we discuss the possible connection ...

  17. Measurement of Entrained Air Bubbles and Vortices in Breaking Waves


    大塚, 淳一; 渡部, 靖憲; Junichi, Otsuka; Yasunori, Watanabe; 北海道大学大学院工学研究科; School of Engineering, Hokkaido University


    Breaking waves produce numbers of vortices through a jet splashing process and also entrain many air bubbles, forming complicated air-water two-phase turbulent flow field in a surf zone. In this research, a simultaneous velocity measurement technique of water and bubble flows in breaking waves is developed for characterizing water-bubble interactions within vortices in a surf zone. The bubbles and neutral buoyant tracers are separately recorded by two different digital video cameras on the ba...

  18. Simulating living organisms with populations of point vortices

    Energy Technology Data Exchange (ETDEWEB)

    Schmieder, R.W.


    The author has found that time-averaged images of small populations of point vortices can exhibit motions suggestive of the behavior of individual organisms. As an example, the author shows that collections of point vortices confined in a box and subjected to heating can generate patterns that are broadly similar to interspecies defense in certain sea anemones. It is speculated that other simple dynamical systems can be found to produce similar complex organism-like behavior.

  19. Secondary vortex formation in ring vortices in free jets (United States)

    Schneider, E. M.


    Secondary vortex formation is examined as a basic component of the turbulent condition of vortex and laminar currents. Dyed fluid ring vortices are used to investigate formation moving against an object and in an unobstructed environment. Part of the vortex structure of jets can be explained by means of secondary vortices, and the sound spectrum both of a single turbulent vortex and of jets is influenced by secondary vortex formation.

  20. Onset of Vortices in Thin Superconducting Strips and Wires

    CERN Document Server

    Aranson, I S; Shapiro, B Y


    Spontaneous nucleation and the consequent penetration of vortices into thin superconducting films and wires, subjected to a magnetic field, can be considered as a nonlinear stage of primary instability of the current-carrying superconducting state. The development of the instability leads to the formation of a chain of vortices in strips and helicoidal vortex lines in wires. The boundary of instability was obtained analytically. The nonlinear stage was investigated by simulations of the time-dependent generalized Ginzburg-Landau equation.

  1. Quantum vortices in systems obeying a generalized exclusion principle. (United States)

    Kaniadakis, G; Scarfone, A M


    The paper deals with a planar particle system obeying a generalized exclusion principle (EP) and governed, in the mean field approximation, by a nonlinear Schrödinger equation. We show that the EP involves a mathematically simple and physically transparent mechanism, which allows the genesis of quantum vortices in the system. We obtain in a closed form the shape of the vortices and investigate its main physical properties.

  2. The role of vortices in animal locomotion in fluids

    Directory of Open Access Journals (Sweden)

    Dvořák R.


    Full Text Available The aim of this paper is to show the significance of vortices in animal locomotion in fluids on two deliberately chosen examples. The first example concerns lift generation by bird and insect wings, the second example briefly mentiones swimming and walking on water. In all the examples, the vortices generated by the moving animal impart the necessary momentum to the surrounding fluid, the reaction to which is the force moving or lifting the animal.

  3. Static and dynamic properties of heavily doped quantum vortices (United States)

    Pshenichnyuk, I. A.


    Quantum vortices in superfluids may capture matter and deposit it inside their core. By doping vortices with foreign particles one can effectively visualize them and study them experimentally. To acquire a better understanding of the interaction between quantum vortices and matter, and clarify the details of recent experiments, the properties of doped vortices are investigated here theoretically in the regimes where the doping mass becomes close to the total mass of superfluid particles forming a vortex. Such formations are dynamically stable and, possessing both vorticity and enhanced inertia, demonstrate properties that are different from the pure vortex case. The goal of this paper is to define and investigate the universal aspects of heavily doped vortex behavior, which can be realized in different types of quantum mixtures. The proposed 3D model is based on a system of coupled semiclassical matter wave equations that are solved numerically in a wide range of physical parameters. The size, geometry and binding energy of dopants in different regimes are discussed. The coupled motion of a vortex–dopant complex and decoupling conditions are studied. The reconnection of vortices, taken as an example of a fundamental process responsible for the evolution of a quantum turbulent state, is modeled to illustrate the difference between the light and heavy doping cases.

  4. A numerical study on helical vortices induced by a short twisted tape in a circular pipe

    Directory of Open Access Journals (Sweden)

    Wen Liu


    Full Text Available Helical vortices, as one kind of secondary flows, are recently observed downstream of the short twisted tape. The behaviors of vortices, which have significant effects on the efficiency of twisted tape, are not well understood. As such, the formation and development of helical vortices induced by the short twisted tape are studied numerically. The results show that two symmetrical stable helical vortices are present downstream of the twisted tape. The values of radial velocities cannot be neglected due to the presence of the vortices. The vortices form in the twisted tape and remain the structure downstream of the twisted tape. Torsion promotes the formation of helical vortices. The intensities of helical vortices decay along the streamwise direction. With the increasing Reynolds numbers, the intensities of helical vortices increase, and the trend is in agreement with the swirl intensities. The intensities of helical vortices decay slowly compared with the intensities of swirling flow.

  5. Complex Convective Thermal Fluxes and Vorticity Structure (United States)

    Redondo, Jose M.; Tellez, Jackson; Sotillos, Laura; Lopez Gonzalez-Nieto, Pilar; Sanchez, Jesus M.; Furmanek, Petr; Diez, Margarita


    Local Diffusion and the topological structure of vorticity and velocity fields is measured in the transition from a homogeneous linearly stratified fluid to a cellular or layered structure by means of convective cooling and/or heating[1,2]. Patterns arise by setting up a convective flow generated by an array of Thermoelectric devices (Peltier/Seebeck cells) these are controlled by thermal PID generating a buoyant heat flux [2]. The experiments described here investigate high Prandtl number mixing using brine and fresh water in order to form density interfaces and low Prandtl number mixing with temperature gradients. The set of dimensionless parameters define conditions of numeric and small scale laboratory modeling of environmental flows. Fields of velocity, density and their gradients were computed and visualized [3,4]. When convective heating and cooling takes place the combination of internal waves and buoyant turbulence is much more complicated if the Rayleigh and Reynolds numbers are high in order to study entrainment and mixing. Using ESS and selfsimilarity structures in the velocity and vorticity fieds and intermittency [3,5] that forms in the non-homogeneous flow is related to mixing and stiring. The evolution of the mixing fronts are compared and the topological characteristics of the merging of plumes and jets in different configurations presenting detailed comparison of the evolution of RM and RT, Jets and Plumes in overall mixing. The relation between structure functions, fractal analysis and spectral analysis can be very useful to determine the evolution of scales. Experimental and numerical results on the advance of a mixing or nonmixing front occurring at a density interface due to body forces [6]and gravitational acceleration are analyzed considering the fractal and spectral structure of the fronts like in removable plate experiments for Rayleigh-Taylor flows. The evolution of the turbulent mixing layer and its complex configuration is studied

  6. Efficiency of coherent vortices to trap dust particles in the solar nebula

    Directory of Open Access Journals (Sweden)

    Chavanis Pierre-Henri


    Full Text Available We develop the idea proposed by Barge & Sommeria (1995 that large-scale vortices present in the solar nebula can concentrate dust particles and facilitate the formation of planetesimals and planets. We introduce an exact vortex solution of the incompressible 2D Euler equation (Kida vortex and study the motion of dust particles in that vortex. In particular, we derive an analytical expression of the capture time as a function of the friction coefficient and determine the parameters leading to an optimal capture.


    Energy Technology Data Exchange (ETDEWEB)

    Lyra, Wladimir [Jet Propulsion Laboratory, California Institute of Technology, 4800 Oak Grove Drive, Pasadena, CA 91109 (United States); Lin, Min-Kai, E-mail:, E-mail: [Canadian Institute for Theoretical Astrophysics, 60 St. George Street, Toronto, Ontario M5S 3H8 (Canada)


    The Atacama Large Millimeter Array has returned images of transitional disks in which large asymmetries are seen in the distribution of millimeter sized dust in the outer disk. The explanation in vogue borrows from the vortex literature and suggests that these asymmetries are the result of dust trapping in giant vortices, excited via Rossby wave instabilities at planetary gap edges. Due to the drag force, dust trapped in vortices will accumulate in the center and diffusion is needed to maintain a steady state over the lifetime of the disk. While previous work derived semi-analytical models of the process, in this paper we provide analytical steady-steady solutions. Exact solutions exist for certain vortex models. The solution is determined by the vortex rotation profile, the gas scale height, the vortex aspect ratio, and the ratio of dust diffusion to gas-dust friction. In principle, all of these quantities can be derived from observations, which would validate the model and also provide constrains on the strength of the turbulence inside the vortex core. Based on our solution, we derive quantities such as the gas-dust contrast, the trapped dust mass, and the dust contrast at the same orbital location. We apply our model to the recently imaged Oph IRS 48 system, finding values within the range of the observational uncertainties.

  8. Characteristics and controllability of vortices in ferromagnetics, ferroelectrics, and multiferroics (United States)

    Zheng, Yue; Chen, W. J.


    Topological defects in condensed matter are attracting e significant attention due to their important role in phase transition and their fascinating characteristics. Among the various types of matter, ferroics which possess a switchable physical characteristic and form domain structure are ideal systems to form topological defects. In particular, a special class of topological defects—vortices—have been found to commonly exist in ferroics. They often manifest themselves as singular regions where domains merge in large systems, or stabilize as novel order states instead of forming domain structures in small enough systems. Understanding the characteristics and controllability of vortices in ferroics can provide us with deeper insight into the phase transition of condensed matter and also exciting opportunities in designing novel functional devices such as nano-memories, sensors, and transducers based on topological defects. In this review, we summarize the recent experimental and theoretical progress in ferroic vortices, with emphasis on those spin/dipole vortices formed in nanoscale ferromagnetics and ferroelectrics, and those structural domain vortices formed in multiferroic hexagonal manganites. We begin with an overview of this field. The fundamental concepts of ferroic vortices, followed by the theoretical simulation and experimental methods to explore ferroic vortices, are then introduced. The various characteristics of vortices (e.g. formation mechanisms, static/dynamic features, and electronic properties) and their controllability (e.g. by size, geometry, external thermal, electrical, magnetic, or mechanical fields) in ferromagnetics, ferroelectrics, and multiferroics are discussed in detail in individual sections. Finally, we conclude this review with an outlook on this rapidly developing field.

  9. Influence of 3D Guide Vanes on the Channel Vortices in the Runner of a Francis Turbine (United States)

    Liu, Shuhong; Zhang, Liang; Wu, Yulin; Luo, Xianwu; Nishi, Michihiro

    It is known that the pressure fluctuation in the runner will become large when a Francis turbine operates at the low flow rate and high head. One of the reasons is the occurrence of channel vortices, which are caused by the three-dimensional flow separating from the suction side of runner blades. In this study, two three-dimensional guide vanes (1# 3D GV, 2# 3D GV) are designed so as to depress the channel vortices and improve the operation performance of a Francis turbine. The flow rate equation for 3D GV is derived firstly in this paper. Then, in order to show the influence of 3D GV on the turbine characteristic, performance test and video-recording of the channel vortices are conducted. Numerical simulation for a part load operation is applied finally to the entire turbine flow passage (from the inlet of spiral case to the outlet of draft tube) using the RNG k-ɛ turbulence model to make clear the three-dimensional internal flow. From evaluation of both the experimental and calculation results, it is noted that the channel vortices from the blade suction side were suppressed effectively by 3D GV, and the turbine efficiency with 3D GV was 0.41 % higher than that with the conventional 2D GV.

  10. Understanding Hurricane Movement from a Potential Vorticity Perspective: a Numerical Model and AN Observational Study. (United States)

    Wu, Chun-Chieh

    In the first part of this thesis, we attempt to isolate the effect of background vertical shear. The hurricane is represented in a two-layer quasigeostrophic model as a point source of mass and zero potential vorticity air in the upper layer, collocated with a point cyclone in the lower layer. The model results show that Northern Hemisphere tropical cyclones should have a component of drift relative to the mean flow in a direction to the left of the background vertical shear. The effect of weak shear is found to be at least as strong as the beta effect, and the effect is maximized by a certain optimal ambient shear. The behavior of the model is sensitive to the thickness ratio of the two layers and is less sensitive to the ratio of the vortices' horizontal scale to the radius of deformation. Storms with stronger negative potential vorticity anomalies tend to exhibit more vortex drift. The validity of balance dynamics in the tropics also allows us to explore the dynamics of hurricanes using the potential vorticity (PV) framework. In the second part of this thesis, three observational case studies (Hurricane Bob and Tropical Storm Ana of 1991, and Hurricane Andrew of 1992) have been performed to demonstrate the use of PV diagnostics of hurricane movement from the twice-daily National Meteorological Center Northen Hemisphere final analyses gridded datasets. Using the seasonal climatology as the mean reference state, piecewise potential vorticity inversions are performed under the nonlinear balance condition. By examining the balanced flows at the central position of the hurricane, one can identify which PV perturbation has the most influence on hurricane movement. We also define the hurricane advection flow as the balanced flow (in the center of the storm) associated with the whole PV in the troposphere, except for the PV anomaly of the hurricane itself. The results from the observational study show that such a steering wind is a very good approximation to the real


    KAUST Repository

    Attili, Antonio


    The alignment of vorticity and gradients of conserved and reactive scalars with the eigenvectors of the strain rate tensor (i.e., the principal strains) is investigated in a direct numerical simulation of a turbulent nonpremixed flame achieving a Taylor’s scale Reynolds number in the range 100≤Reλ≤150 (Attili et al. Comb. Flame, 161, 2014). The vorticity vector displays a pronounced tendency to align with the direction of the intermediate strain. These alignment statistics are in almost perfect agreement with those in homogeneous isotropic turbulence (Ashurst et al. Physics of Fluids 30, 1987) and differ significantly from the results obtained in other nonpremixed flames in which vorticity alignment with the most extensive strain was observed (Boratavet al. Physics of Fluids 8, 1996). The gradients of conserved and reactive scalars align with the most compressive strain. It is worth noting that conditioning on the local values of the mixture fraction, or equivalently conditioning on the distance from the flame sheet, does not affect the statistics. Our results suggest that turbulence overshadows the effects of heat release and chemical reactions. This may be due to the larger Reynolds number achieved in the present study compared to that in previous works.

  12. Dynamic, Thermodynamic and Vorticity Budget Analysis of a Simulated Tornado (United States)

    Yin, Yue

    Data from a very high resolution (30m grid spacing) simulation of a long-lived EF5 tornado embedded in a supercell was used to examine the dynamic and thermodynamic quantities associated with the evolution of the simulated tornado. A Eulerian vertical vorticity budget analysis was performed in a volume bounding the lowest grid layer inside the tornado at various stages to better understand the possible vorticity sources for the low-level tornado, particularly the role of small-scale vortices present primarily along the forward-flank downdraft boundary. The dynamic and thermodynamic fields exhibit similar features to past studies such as slightly positive pressure perturbations along the rear-flank downdraft boundary, small-scale downdrafts with positive potential temperature perturbation to the southwest of the tornado, two-celled structure of the tornado, etc. The rear-flank downdraft in this simulation differs from the classic conceptual model because the vertical velocity was found to be either close to zero or slightly positive in a distinctive region wrapping partially around the major updraft, where previous studies often found a prominent rear-flank downdraft. The vertical vorticity budget analysis shows that the stretching term and the vertical flux term largely oppose each other with nearly the same magnitude throughout the analysis period. The tilting term is almost zero (though slightly positive) throughout the genesis stage, and makes a small and positive contribution in the mature and late mature phases of the tornado. The horizontal flux term makes the greatest contribution in the vertical vorticity source for the tornado during the genesis stage and a significant contribution during the maintenance period. This suggests that the small-scale vortices present along the forward-flank downdraft boundary flowing towards and merging with the tornado are the major vertical vorticity source for the development of the tornado. This, and the lack of a cohesive

  13. Vortical structures in pool fires: Observation, speculation, and simulation

    Energy Technology Data Exchange (ETDEWEB)

    Tieszen, S.R.; Nicolette, V.F.; Gritzo, L.A.; Moya, J.L. [Sandia National Labs., Albuquerque, NM (United States); Holen, J.K. [SINTEF/NTH, Trondheim (Norway). Div. Thermodynamics; Murray, D. [Naval Air Warfare Center, China Lake, CA (United States)


    While all fires are complex and involve many phenomena, this report is limited to large, turbulent liquid-hydrocarbon pool fires. Large, liquid-hydrocarbon pool fires present a risk in petrochemical storage and processing facilities and transportation systems that contain large amounts of liquid hydrocarbons. This report describes observations, speculations, and numerical simulations of vortical structures in pool fires. Vortical structures are observed in fires with length scales ranging from those that bend millimeter-thick flame zones to those that entrain air many meters from the edge of the fire to its centerline. The authors propose that baroclinic vorticity generation is primarily responsible for production of rotational motion at small scale and that amalgamation is responsible for the production of large-scale rotational structures from the myriad of small-scale structures. Numerical simulations show that vortical structures having time-mean definitions can be resolved with a Reynolds-Average Navier-Stokes (RANS) approach. However, for vortical structures without time-mean definition, RANS is inappropriate, and another technique, such as Large Eddy Simulation (LES), should be employed. 39 refs., 52 figs., 3 tabs.

  14. Pinned vorticity in rotating superfluids, with application to neutron stars

    Energy Technology Data Exchange (ETDEWEB)

    Pines, D.; Shaham, J. (Illinois Univ., Urbana (USA). Dept. of Physics); Alpar, M.A.; Anderson, P.W.


    The dynamic consequences of the existence of pinned vorticity in a rotating superfluid are studied by means of a simple model: the behavior of a rotating cylinder which contains a uniform region of either weakly or strongly pinned vorticity and which is being spun up or spun down by an external torque. It is shown that in the case of strong pinning, spin down can lead to periodic jumps (glitches) in the rotation frequency of the cylinder, followed by quasi-oscillatory relaxation, while in the case of weak pinning no glitches occur unless the cylinder is shaken so violently that vortices unpin. We conclude that the giant glitches and post-glitch behavior observed in the Vela pulsar may be explained by the sudden release of some 10% of the strongly pinned vortices in the neutron crust every few years as a result of pulsar spin down. We further suggest that the post-glitch behavior observed in the Crab pulsar can be explained if the macroglitches represent vorticity jumps induced by small starquakes in the weakly pinned vortex region expected in the crust of a young neutron star, and that the differences in ''glitch'' behavior of the Crab, Vela, and older pulsars may be explained on evolutionary grounds.

  15. Vortices and sound waves in superfluids


    Lee, Kimyeong


    We consider the dynamics of vortex strings and sound waves in superfluids in the phenomenological Landau-Ginzburg equation. We first derive the vortex equation where the velocity of a vortex is determined by the average fluid velocity and the density gradient near the vortex. We then derive the effective action for vortex strings and sound waves by the dual formulation. The effective action might be useful in calculating the emission rate of sound waves by moving vortex strings.

  16. Ultrasound Vector Flow Imaging: Part I: Sequential Systems

    DEFF Research Database (Denmark)

    Jensen, Jørgen Arendt; Nikolov, Svetoslav Ivanov; Yu, Alfred C. H.


    The paper gives a review of the most important methods for blood velocity vector flow imaging (VFI) for conventional, sequential data acquisition. This includes multibeam methods, speckle tracking, transverse oscillation, color flow mapping derived vector flow imaging, directional beamforming, an...

  17. Calculation of three-dimensional (3-D) internal flow by means of the velocity-vorticity formulation on a staggered grid (United States)

    Stremel, Paul M.


    A method has been developed to accurately compute the viscous flow in three-dimensional (3-D) enclosures. This method is the 3-D extension of a two-dimensional (2-D) method developed for the calculation of flow over airfoils. The 2-D method has been tested extensively and has been shown to accurately reproduce experimental results. As in the 2-D method, the 3-D method provides for the non-iterative solution of the incompressible Navier-Stokes equations by means of a fully coupled implicit technique. The solution is calculated on a body fitted computational mesh incorporating a staggered grid methodology. In the staggered grid method, the three components of vorticity are defined at the centers of the computational cell sides, while the velocity components are defined as normal vectors at the centers of the computational cell faces. The staggered grid orientation provides for the accurate definition of the vorticity components at the vorticity locations, the divergence of vorticity at the mesh cell nodes and the conservation of mass at the mesh cell centers. The solution is obtained by utilizing a fractional step solution technique in the three coordinate directions. The boundary conditions for the vorticity and velocity are calculated implicitly as part of the solution. The method provides for the non-iterative solution of the flow field and satisfies the conservation of mass and divergence of vorticity to machine zero at each time step. To test the method, the calculation of simple driven cavity flows have been computed. The driven cavity flow is defined as the flow in an enclosure driven by a moving upper plate at the top of the enclosure. To demonstrate the ability of the method to predict the flow in arbitrary cavities, results will he shown for both cubic and curved cavities.

  18. On Chirality of the Vorticity of the Universe

    Directory of Open Access Journals (Sweden)

    Davor Palle


    Full Text Available The presence of dark energy in the Universe challenges the Einstein’s theory of gravity at cosmic scales. It motivates the inclusion of rotational degrees of freedom in the Einstein–Cartan gravity, representing the minimal and the most natural extension of the General Relativity. One can, consequently, expect the violation of the cosmic isotropy by the rotating Universe. We study chirality of the vorticity of the Universe within the Einstein–Cartan cosmology. The role of the spin of fermion species during the evolution of the Universe is studied by averaged spin densities and Einstein–Cartan equations. It is shown that spin density of the light Majorana neutrinos acts as a seed for vorticity at early stages of the evolution of the Universe. Its chirality can be evaluated in the vicinity of the spacelike infinity. It turns out that vorticity of the Universe has right-handed chirality.

  19. Pair interactions of heavy vortices in quantum fluids (United States)

    Pshenichnyuk, Ivan A.


    The dynamics of quantum vortex pairs carrying heavy doping matter trapped inside their cores is studied. The nonlinear classical matter field formalism is used to build a universal mathematical model of a heavy vortex applicable to different types of quantum mixtures. It is shown how the usual vortex dynamics typical for undoped pairs qualitatively changes when heavy dopants are used: heavy vortices with opposite topological charges (chiralities) attract each other, while vortices with the same charge are repelled. The force responsible for such behavior appears as a result of superposition of vortices velocity fields in the presence of doping substance and can be considered as a special realization of the Magnus effect. The force is evaluated quantitatively and its inverse proportionality to the distance is demonstrated. The mechanism described in this paper gives an example of how a light nonlinear classical field may realize repulsive and attractive interactions between embedded heavy impurities.

  20. Multicharged optical vortices induced in a dissipative atomic vapor system

    CERN Document Server

    Zhang, Yiqi; Wu, Zhenkun; Yuan, Chenzhi; Wang, Ruimin; Lu, Keqing; Zhang, Yanpeng


    We investigate numerically the dynamics of optical vortex beams carrying different topological charges, launched in a dissipative three level ladder type nonlinear atomic vapor. We impose the electromagnetically induced transparency (EIT) condition on the medium. Linear, cubic, and quintic susceptibilities, considered simultaneously with the dressing effect, are included in the analysis. Generally, the beams slowly expand during propagation and new vortices are induced, commonly appearing in oppositely charged pairs. We demonstrate that not only the form and the topological charge of the incident beam, but also its growing size in the medium greatly affect the formation and evolution of vortices. We formulate common rules for finding the number of induced vortices and the corresponding rotation directions, stemming from the initial conditions of various incident beams, as well as from the dynamical aspects of their propagation. The net topological charge of the vortex is conserved during propagation, as it sh...

  1. Inelastic scattering of xenon atoms by quantized vortices in superfluids

    CERN Document Server

    Pshenichnyuk, I A


    We study inelastic interactions of particles with quantized vortices in superfluids by using a semi-classical matter wave theory that is analogous to the Landau two-fluid equations, but allows for the vortex dynamics. The research is motivated by recent experiments on xenon doped helium nanodroplets that show clustering of the impurities along the vortex cores. We numerically simulate the dynamics of trapping and interactions of xenon atoms by quantized vortices in superfluid helium and the obtained results can be extended to scattering of other impurities by quantized vortices. Different energies and impact parameters of incident particles are considered. We show that inelastic scattering is closely linked to the generation of Kelvin waves along a quantized vortex during the interaction even if there is no capture. The capture criterion of an impurity is formulated in terms of the binding energy.

  2. Vorticity scaling and intermittency in drift-interchange plasma turbulence (United States)

    Dura, P. D.; Hnat, B.; Robinson, J.; Dendy, R. O.


    The effects of spatially varying magnetic field strength on the scaling properties of plasma turbulence, modelled by an extended form of Hasegawa-Wakatani model, are investigated. We study changes in the intermittency of the velocity, density, and vorticity fields, as functions of the magnetic field inhomogeneity C =-∂ ln B/∂x. While the velocity fluctuations are always self-similar and their scaling is unaffected by the value of C, the intermittency levels in density and vorticity change with parameter C, reflecting morphological changes in the coherent structures due to the interchange mechanism. Given the centrality of vorticity in conditioning plasma transport, this result is of interest in scaling the results of transport measurements and simulations in tokamak edge plasmas, where drift-interchange turbulence in the presence of a magnetic field gradient is likely to occur.

  3. The Evaluation of the Boundary Vorticity by URANS and LES Methods

    Directory of Open Access Journals (Sweden)

    Ion MALAEL


    Full Text Available The role of concentrated vorticity in fluid dynamics phenomena, concerning both the vorticity creation at the boundary and the response to the flow field is not wholly understood. The Lighthill describes the vorticity production at a solid boundary as a slow diffusion of the vorticity similar to the Fourier heat conduction. In the paper it is shown that this mechanism associated to URANS method is not applied to the concentrated vorticity case, and the LES method better reproduces the flows involving concentrations vorticity.

  4. Wake development behind paired wings with tip and root trailing vortices: consequences for animal flight force estimates.

    Directory of Open Access Journals (Sweden)

    Jan T Horstmann

    Full Text Available Recent experiments on flapping flight in animals have shown that a variety of unrelated species shed a wake behind left and right wings consisting of both tip and root vortices. Here we present an investigation using Particle Image Velocimetry (PIV of the behaviour and interaction of trailing vortices shed by paired, fixed wings that simplify and mimic the wake of a flying animal with a non-lifting body. We measured flow velocities at five positions downstream of two adjacent NACA 0012 aerofoils and systematically varied aspect ratio, the gap between the wings (corresponding to the width of a non-lifting body, angle of attack, and the Reynolds number. The range of aspect ratios and Reynolds number where chosen to be relevant to natural fliers and swimmers, and insect flight in particular. We show that the wake behind the paired wings deformed as a consequence of the induced flow distribution such that the wingtip vortices convected downwards while the root vortices twist around each other. Vortex interaction and wake deformation became more pronounced further downstream of the wing, so the positioning of PIV measurement planes in experiments on flying animals has an important effect on subsequent force estimates due to rotating induced flow vectors. Wake deformation was most severe behind wings with lower aspect ratios and when the distance between the wings was small, suggesting that animals that match this description constitute high-risk groups in terms of measurement error. Our results, therefore, have significant implications for experimental design where wake measurements are used to estimate forces generated in animal flight. In particular, the downstream distance of the measurement plane should be minimised, notwithstanding the animal welfare constraints when measuring the wake behind flying animals.

  5. Comparison of homologous and heterologous prime-boost immunizations combining MVA-vectored and plant-derived VP2 as a strategy against IBDV. (United States)

    Richetta, Matías; Gómez, Evangelina; Lucero, María Soledad; Chimeno Zoth, Silvina; Gravisaco, María José; Calamante, Gabriela; Berinstein, Analía


    Different immunogens such as subunit, DNA or live viral-vectored vaccines against Infectious Bursal Disease virus (IBDV) have been evaluated in the last years. However, the heterologous prime-boost approach using recombinant modified vaccinia Ankara virus (rMVA), which has shown promising results in both mammals and chickens, has not been tried against this pathogen yet. IBD is a highly contagious and immunosuppressive disease of poultry that affects mainly young chicks. It is caused by IBDV, a double-stranded RNA virus carrying its main antigenic epitopes on the capsid protein VP2. Our objective was to evaluate the immune response elicited by two heterologous prime-boost schemes combining an rMVA carrying the VP2 mature gene (rVP2) and a recombinant VP2 protein produced in Nicotiana benthamiana (pVP2), and to compare them with the performance of the homologous pVP2-pVP2 scheme usually used in our laboratory. The SPF chickens immunized with the three evaluated schemes elicited significantly higher anti-VP2 antibody titers (p<0.001) and seroneutralizing titers (p<0.05) and had less T-cell infiltration (p<0.001), histological damage (p<0.001) and IBDV particles (p<0.001) in their bursae of Fabricius when compared with control groups. No significant differences were found between both heterologous schemes and the homologous one. However, the rVP2-pVP2 scheme showed significantly higher anti-VP2 antibody titers than pVP2-rVP2 and a similar tendency was found in the seroneutralization assay. Conversely, pVP2-rVP2 had the best performance when evaluated through bursal parameters despite having a less potent humoral immune response. These findings suggest that the order in which rVP2 and pVP2 are combined can influence the immune response obtained. Besides, the lack of a strong humoral immune response did not lessen the ability to protect from IBDV challenge. Therefore, further research is needed to evaluate the mechanisms by which these immunogens are working in order to

  6. New recombinant serotypes of AAV vectors. (United States)

    Gao, Guangping; Vandenberghe, Luk H; Wilson, James M


    AAV based vectors can achieve stable gene transfer with minimal vector related toxicities. AAV serotype 2 (AAV2) is the first AAV that was vectored for gene transfer applications. However, the restricted tissue tropism of AAV and its low transduction efficiency have limited its further development as vector. Recent studies using vectors derived from alternative AAV serotypes such as AAV1, 4, 5 and 6 have shown improved potency and broadened tropism of the AAV vector by packaging the same vector genome with different AAV capsids. In an attempt to search for potent AAV vectors with enhanced performance profiles, molecular techniques were employed for the detection and isolation of endogenous AAVs from a variety of human and non-human primate (NHP) tissues. A family of novel primate AAVs consisting of 110 non-redundant species of proviral sequences was discovered and turned to be prevalent in 18-19% of the tissues evaluated. Phylogenetic and functional analyses revealed that primate AAVs are segregated into clades based on phylogenetic relatedness. The members within a clade share functional and serological properties. Initial evaluation in mouse models of vectors based on these novel AAVs for tissue tropism and gene transfer potency led to the identification of some vector with improved gene transfer to different target tissues. Gene therapy treatment of several mouse and canine models with novel AAV vectors achieved long term phenotypic corrections. Vectors based on new primate AAVs could become the next generation of efficient gene transfer vehicles for various gene therapy applications.

  7. Nonlinear Dynamics of Vortices in Different Types of Grain Boundaries

    Energy Technology Data Exchange (ETDEWEB)

    Sheikhzada, Ahmad [Old Dominion Univ., Norfolk, VA (United States)


    As a major component of linear particle accelerators, superconducting radio-frequency (SRF) resonator cavities are required to operate with lowest energy dissipation and highest accelerating gradient. SRF cavities are made of polycrystalline materials in which grain boundaries can limit maximum RF currents and produce additional power dissipation sources due to local penetration of Josephson vortices. The essential physics of vortex penetration and mechanisms of dissipation of vortices driven by strong RF currents along networks of grain boundaries and their contribution to the residual surface resistance have not been well understood. To evaluate how GBs can limit the performance of SRF materials, particularly Nb and Nb3Sn, we performed extensive numerical simulations of nonlinear dynamics of Josephson vortices in grain boundaries under strong dc and RF fields. The RF power due to penetration of vortices both in weakly-coupled and strongly-coupled grain boundaries was calculated as functions of the RF field and frequency. The result of this calculation manifested a quadratic dependence of power to field amplitude at strong RF currents, an illustration of resistive behavior of grain boundaries. Our calculations also showed that the surface resistance is a complicated function of field controlled by penetration and annihilation of vortices and antivortices in strong RF fields which ultimately saturates to normal resistivity of grain boundary. We found that Cherenkov radiation of rapidly moving vortices in grain boundaries can produce a new instability causing generation of expanding vortex-antivortex pair which ultimately drives the entire GB in a resistive state. This effect is more pronounced in polycrystalline thin film and multilayer coating structures in which it can cause significant increase in power dissipation and results in hysteresis effects in I-V characteristics, particularly at low temperatures.

  8. Recombinant alphaviruses as vectors for anti-tumour and anti-microbial immunotherapy

    NARCIS (Netherlands)

    Riezebos-Brilman, A; de Mare, A; Bungener, L; Huckriede, A; Wischut, J; Daemen, T

    Background: Vectors derived from alphaviruses are gaining interest for their high transfection potency and strong immunogenicity. Objectives: After a brief introduction on alphaviruses and their vectors, an overview is given on current preclinical immunotherapy studies using vector systems based on

  9. Manipulation of pancake vortices by rotating a Josephson vortex lattice

    Energy Technology Data Exchange (ETDEWEB)

    Crisan, A [Department of Metallurgy and Materials, University of Birmingham, Edgbaston, Birmingham B15 2TT (United Kingdom); Bending, S J [Department of Physics, University of Bath, Claverton Down, Bath BA2 7AY (United Kingdom); Tamegai, T [Department of Applied Physics, University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-8627 (Japan)


    Scanning Hall probe microscopy has been used to demonstrate the manipulation of pancake vortices by rotating the Josephson vortex lattice in Bi{sub 2}Sr{sub 2}CaCu{sub 2}O{sub 8+{delta}} single crystals in the interacting crossing lattices regime. Creation of one-dimensional pancake vortex chains trapped on Josephson vortices, and the subsequent rotation of the chains were realized by independently controlling magnetic fields in three orthogonal directions. The anisotropy parameter determined from the in-plane distances between vortex chains in various in-plane fields is consistent with commonly accepted values.

  10. (Non)-renormalization of the chiral vortical effect coefficient

    Energy Technology Data Exchange (ETDEWEB)

    Golkar, Siavash; Son, Dam T. [Kadanoff Center for Theoretical Physics and Enrico Fermi Institute, University of Chicago,5640 South Ellis Ave., Chicago, IL 60637 (United States)


    We show using diagramtic arguments that in some (but not all) cases, the temperature dependent part of the chiral vortical effect coefficient is independent of the coupling constant. An interpretation of this result in terms of quantization in the effective 3 dimensional Chern-Simons theory is also given. In the language of 3D, dimensionally reduced theory, the value of the chiral vortical coefficient is related to the formula ∑{sub n=1}{sup ∞}n=−1/12. We also show that in the presence of dynamical gauge fields, the CVE coefficient is not protected from renormalization, even in the large N limit.

  11. Intrinsic nonadiabatic topological torque in magnetic skyrmions and vortices

    KAUST Repository

    Akosa, Collins Ashu


    We propose that topological spin currents flowing in topologically nontrivial magnetic textures, such as magnetic skyrmions and vortices, produce an intrinsic nonadiabatic torque of the form Tt∼[(∂xm×∂ym)·m]∂ym. We show that this torque, which is absent in one-dimensional domain walls and/or nontopological textures, is responsible for the enhanced nonadiabaticity parameter observed in magnetic vortices compared to one-dimensional textures. The impact of this torque on the motion of magnetic skyrmions is expected to be crucial, especially to determine their robustness against defects and pinning centers.

  12. Unfolding of vortices into topological stripes in a multiferroic material. (United States)

    Wang, X; Mostovoy, M; Han, M G; Horibe, Y; Aoki, T; Zhu, Y; Cheong, S-W


    Multiferroic hexagonal RMnO(3) (R=rare earths) crystals exhibit dense networks of vortex lines at which six domain walls merge. While the domain walls can be readily moved with an applied electric field, the vortex cores so far have been impossible to control. Our experiments demonstrate that shear strain induces a Magnus-type force pulling vortices and antivortices in opposite directions and unfolding them into a topological stripe domain state. We discuss the analogy between this effect and the current-driven dynamics of vortices in superconductors and superfluids.

  13. On the interaction between two oppositely signed, shielded, monopolar vortices

    DEFF Research Database (Denmark)

    Schmidt, M.R.; Beckers, M.; Nielsen, A.H.


    solutions of the two-dimensional Navier-Stokes equations. A comparative study between the laboratory experiments and numerical simulations is performed. The vorticity distribution measured in the early stage of the evolution in the laboratory is used as initial data for the simulations, and an additional...... damping term in the Navier-Stokes equations, that accounts for the vertical diffusion in the laboratory experiments, is used. The results show that, depending on the initial separation between the vortices, the shields of the monopoles are peeled off and indeed a compact dipole with a linear (omega...

  14. Vorticity Measurement using LG Laser Beams with Orbital Angular Momentum (United States)

    Kooochesfahani, Manoochehr; Pouya, Shahram; Safaripour, Alireza; Ryabtsev, Anton; Dantus, Marcos


    We present direct measurement of vorticity in a fluid flow based on angular velocity measurement of microparticles contained in the fluid. The method uses Laguerre-Gaussian (LG) laser beams that possess orbital angular momentum (OAM), a spatial (azimuthal) modulation of the beam phase front, and takes advantage of the rotational Doppler shift from microparticles intersecting the beam focus. Results are shown for the flow field of solid body rotation, where the flow vorticity is known precisely. This work was supported by AFOSR Award Number FA9550-14-1-0312.

  15. Enhanced transduction of colonic cell lines in vitro and the inflamed colon in mice by viral vectors, derived from adeno-associated virus serotype 2, using virus-microbead conjugates bearing lectin

    Directory of Open Access Journals (Sweden)

    Sano Takeshi


    Full Text Available Abstract Background Virus-mediated delivery of therapeutic transgenes to the inflamed colon holds a great potential to serve as an effective therapeutic strategy for inflammatory bowel disease, since local, long-term expression of the encoded therapeutic proteins in the colorectal system is potentially achievable. Viral vectors, derived from adeno-associated virus (AAV, should be very useful for such therapeutic strategies, particularly because they can establish long-term expression of transgenes. However, few studies have been carried out to investigate the ability of AAV-based vectors to transduce the inflamed colon. Results AAV, derived from adeno-associated virus serotype 2 (AAV2, showed a limited ability to transduce colonic cell lines in vitro when used in free form. No appreciable enhancement of the transduction efficiency was seen when AAV2 particles were attached stably to the surfaces of microbeads and delivered to target cells in the form of AAV2-microbead conjugates. However, the transduction efficiency of these colonic cell lines was enhanced substantially when a lectin, concanavalin A (Con A, was co-attached to the microbead surfaces, to which AAV2 particles had been conjugated. This considerable infectivity enhancement of AAV2-microbead conjugates by the co-attachment of Con A may be derived from the fact that Con A binds to α-D-mannosyl moieties that are commonly and abundantly present in cell-surface carbohydrate chains, allowing the conjugates to associate stably with target cells. Intracolonical administration of free AAV2 or AAV2-microbead conjugates without Con A into a mouse colitis model by enema showed very poor transduction of the colonic tissue. In contrast, the delivery of AAV2 in the form of AAV2-microbead conjugates bearing Con A resulted in efficient transduction of the inflamed colon. Conclusion AAV2-microbead conjugates bearing Con A can serve as efficient gene transfer agents both for poorly permissive colonic

  16. Quantized vortices in interacting gauge theories (United States)

    Butera, Salvatore; Valiente, Manuel; Öhberg, Patrik


    We consider a two-dimensional weakly interacting ultracold Bose gas whose constituents are two-level atoms. We study the effects of a synthetic density-dependent gauge field that arises from laser-matter coupling in the adiabatic limit with a laser configuration such that the single-particle zeroth-order vector potential corresponds to a constant synthetic magnetic field. We find a new exotic type of current nonlinearity in the Gross-Pitaevskii equation which affects the dynamics of the order parameter of the condensate. We investigate the rotational properties of this system in the Thomas-Fermi limit, focusing in particular on the physical conditions that make the existence of a quantized vortex in the system energetically favourable with respect to the non-rotating solution. We point out that two different physical interpretations can be given to this new nonlinearity: firstly it can be seen as a local modification of the mean field coupling constant, whose value depends on the angular momentum of the condensate. Secondly, it can be interpreted as a density modulated angular velocity given to the cloud. Looking at the problem from both of these viewpoints, we show that the effect of the new nonlinearity is to induce a rotation to the condensate, where the transition from non-rotating to rotating states depends on the density of the cloud.

  17. Crystallized and amorphous vortices in rotating atomic-molecular Bose-Einstein condensates (United States)

    Liu, Chao-Fei; Fan, Heng; Gou, Shih-Chuan; Liu, Wu-Ming


    Vortex is a topological defect with a quantized winding number of the phase in superfluids and superconductors. Here, we investigate the crystallized (triangular, square, honeycomb) and amorphous vortices in rotating atomic-molecular Bose-Einstein condensates (BECs) by using the damped projected Gross-Pitaevskii equation. The amorphous vortices are the result of the considerable deviation induced by the interaction of atomic-molecular vortices. By changing the atom-molecule interaction from attractive to repulsive, the configuration of vortices can change from an overlapped atomic-molecular vortices to carbon-dioxide-type ones, then to atomic vortices with interstitial molecular vortices, and finally into independent separated ones. The Raman detuning can tune the ratio of the atomic vortex to the molecular vortex. We provide a phase diagram of vortices in rotating atomic-molecular BECs as a function of Raman detuning and the strength of atom-molecule interaction.

  18. A (dis)continuous finite element model for generalized 2D vorticity dynamics

    NARCIS (Netherlands)

    Bernsen, E.; Bokhove, Onno; van der Vegt, Jacobus J.W.; Wesseling, P.; Onate, E; Periaux, J.


    A mixed continuous and discontinuous Galerkin finite element discretization has been constructed for a generalized vorticity-streamfunction formulation in two spatial dimensions. This formulation consists of a hyperbolic (potential) vorticity equation and a linear elliptic equation for a (transport)

  19. Twin boundary effects on spontaneous half-quantized vortices in superconducting composite structures (d-dot’s)

    Energy Technology Data Exchange (ETDEWEB)

    Fujita, Norio; Kato, Masaru [Department of Mathematical Sciences, Osaka Prefecture University, 1-1, Gakuencho, Nakaku, Sakai, Osaka 599-8531 (Japan); Ishida, Takekazu [Department of Physics and Electronics, Osaka Prefecture University, 1-1, Gakuencho, Nakaku, Sakai, Osaka 599-8531 (Japan)


    Highlights: • A d-dot is a nano-scaled composite structure that consists of a d- and s-wave SC. • Two-components GL equations with anisotropic effective mass of electrons are derived. • Anisotropic effective mass suppress spontaneous half-quantized vortices in d-dot’s. • Thin junctions are good for spontaneous half-quantized vortices in d-dot’s. - Abstract: We investigate effects of anisotropy of an orthorhombic structure in twin domains on spontaneous half-quantized vortices (SHQVs) in a d-dot, which is a nano-scaled composite structure that consists of a d-wave superconductor embedded in an s-wave matrix. Since YBa{sub 2}Cu{sub 3}O{sub 7−δ} (YBCO) has the orthorhombic structure, there are twin domains separated by twin boundaries (TBs). In order to analyze effects of TBs on SHQVs, we derive two-component Ginzburg–Landau equations, in which electrons in YBCO have anisotropic effective mass. It is found that the magnitude of field around SHQVs is monotonically decreasing with increasing anisotropy of effective mass and finally peak values of the fields become zero. This means that anisotropy of the effective mass suppresses SHQVs and when the anisotropy is too large, SHQVs do not appear in d-dot’s.

  20. The vorticity and angular momentum budgets of Asian summer ...

    Indian Academy of Sciences (India)

    Home; Journals; Journal of Earth System Science; Volume 113; Issue 3. The vorticity and angular momentum ... The flux convergence of omega and relative momenta over the monsoon domain is effectively balanced by pressure torque during the evolution and established phases. Nevertheless, the balance is stronger ...

  1. Tip vorticity reduction and optimization of lifting surfaces

    NARCIS (Netherlands)

    Sparenberg, JA

    In linearized optimization theory, lifting surfaces, moving in an inviscid and incompressible fluid, shed tip vorticity of which the strength has infinite square-root singularities. Here we discuss that an optimization procedure can be coupled to constraints so that the strength of the shed

  2. Compressible dynamic stall vorticity flux control using a dynamic ...

    Indian Academy of Sciences (India)

    be thought of as arising from the rapidly changing strong pressure gradient near the leading edge due to its unsteady motion. Thus, even though the vorticity is produced by the unsteady surface motion, it appears that its subsequent convection through the boundary layer over the airfoil upper surface can only be managed ...

  3. Instability of helical tip vortices in rotor wakes

    DEFF Research Database (Denmark)

    Sørensen, Jens Nørkær


    The conditions for the appearance of instabilities in systems of helical vortices constitute an intriguing problem that still remains partly unsolved. The experimental study of Felli, Camussi & Di Felice (J. Fluid Mech., this issue, vol. 682, 2011, pp. 5-53) has shed new light on some of the basi...

  4. Clustering of heavy particles in vortical flows: a selective review

    Indian Academy of Sciences (India)

    S Ravichandran

    Vortical flows; inertial particles; preferential clustering; sling caustics; particle collisions; warm rain initiation. ... The equation of motion for a small sphere in fluid flow was ... to a Magnus force. The terms with a factor a2Du are called. Faxen corrections, and account for changes in the flow over length scales of the particle size.

  5. Vortices in Bose–Einstein condensates: A review of the ...

    Indian Academy of Sciences (India)

    mode instability; but this is not the only mechanism; (b) the vortex lattice is perfectly triangular right up to the ... themselves in a lattice; this time appears to be independent of temperature; (d) the decay of vortices appears to ..... a state |1〉 to a state |2〉 at a Rabi frequency Ω by using electromagnetic radiation detuned by δ.

  6. The vorticity and angular momentum budgets of Asian summer ...

    Indian Academy of Sciences (India)

    R. Narasimhan (Krishtel eMaging) 1461 1996 Oct 15 13:05:22

    1School of Geography and Geology, McMaster University, Hamilton, ON, Canada L8S 4K1. 2Centre for Atmospheric Sciences, Indian Institute of Technology-Delhi Hauz Khas, New Delhi 110 016, India. The study delineates the vorticity and angular momentum balances of Asian summer monsoon dur- ing the evolution and ...

  7. Generation of tripolar vortical structures on the beta plane

    DEFF Research Database (Denmark)

    Hesthaven, J.S.; Lynov, Jens-Peter; Juul Rasmussen, J.


    A new feature of the long-time evolution of a strong vortex with initially monotonic potential vorticity is found by direct numerical solution of the quasigeostrophic equivalent barotropic equation. Two satellites, which emerge after splitting of an annulus, appear at the vortex periphery. Rotation...

  8. Ionospheric travelling convection vortices observed by the Greenland magnetometer chain

    DEFF Research Database (Denmark)

    Kotsiaros, Stavros; Stolle, Claudia; Friis-Christensen, Eigil


    The Greenland magnetometer array continuously provides geomagnetic variometer data since the early eighties. With the polar cusp passing over it almost every day, the array is suitable to detect ionospheric traveling convection vortices (TCVs), which were rst detected by Friis-Christensen et al...

  9. Vortices in Bose–Einstein condensates: A review of the ...

    Indian Academy of Sciences (India)

    Such vortices have been generated in BEC of alkali atoms by different techniques such as (a) wave function engineering of a two-component BEC, (b) decay of solitons, (c) rotation of a thermal cloud before cooling it below the ... Raman Research Institute, C.V. Raman Avenue, Sadashivanagar, Bangalore 560 080, India ...

  10. Comparing the dynamics of skyrmions and superconducting vortices

    Energy Technology Data Exchange (ETDEWEB)

    Olson Reichhardt, C.J., E-mail: [Theoretical Division, Los Alamos National Laboratory, Los Alamos, NM 87545 (United States); Lin, S.Z. [Theoretical Division, Los Alamos National Laboratory, Los Alamos, NM 87545 (United States); Ray, D. [Theoretical Division, Los Alamos National Laboratory, Los Alamos, NM 87545 (United States); Department of Physics, University of Notre Dame, Notre Dame, IN 46556 (United States); Reichhardt, C. [Theoretical Division, Los Alamos National Laboratory, Los Alamos, NM 87545 (United States)


    Highlights: • We describe similarities and differences between skyrmion and vortex dynamics. • The Magnus force can dramatically alter skyrmion transport. • The pinning becomes very weak when the Magnus force is strong. - Abstract: Vortices in type-II superconductors have attracted enormous attention as ideal systems in which to study nonequilibrium collective phenomena, since the self-ordering of the vortices competes with quenched disorder and thermal effects. Dynamic effects found in vortex systems include depinning, nonequilibrium phase transitions, creep, structural order–disorder transitions, and melting. Understanding vortex dynamics is also important for applications of superconductors which require the vortices either to remain pinned or to move in a controlled fashion. Recently, topological defects called skyrmions have been realized experimentally in chiral magnets. Here we highlight similarities and differences between skyrmion dynamics and vortex dynamics. Many of the previous ideas and experimental setups that have been applied to superconducting vortices can also be used to study skyrmions. We also discuss some of the differences between the two systems, such as the potentially large contribution of the Magnus force in the skyrmion system that can dramatically alter the dynamics and transport properties.

  11. Streaming vorticity flux from oscillating walls with finite amplitude (United States)

    Wu, J. Z.; Wu, X. H.; Wu, J. M.


    How to describe vorticity creation from a moving wall is a long standing problem. This paper discusses relevant issues at the fundamental level. First, it is shown that the concept of 'vorticity flux due to wall acceleration' can be best understood by following fluid particles on the wall rather than observing the flow at fixed spatial points. This is of crucial importance when the time-averaged flux is to be considered. The averaged flux has to be estimated in a wall-fixed frame of reference (in which there is no flux due to wall acceleration at all); or, if an inertial frame of reference is used, the generalized Lagrangian mean (GLM) also gives the same result. Then, for some simple but typical configurations, the time-averaged vorticity flux from a harmonically oscillating wall with finite amplitude is analyzed, without appealing to small perturbation. The main conclusion is that the wall oscillation will produce an additional mean vorticity flux (a fully nonlinear streaming effect), which is partially responsible for the mechanism of vortex flow control by waves. The results provide qualitative explanation for some experimentally and/or computationally observed phenomena.

  12. The decay of wake vortices in the convective boundary layer

    Energy Technology Data Exchange (ETDEWEB)

    Holzaepfel, F.; Gerz, T.; Frech, M.; Doernbrack, A.


    The decay of three wake vortex pairs of B-747 aircraft in a convectively driven atmospheric boundary layer is investigated by means of large-eddy simulations (LES). This situation is considered as being hazardous as the updraft velocities of a thermal may compensate the induced descent speed of the vortex pair resulting in vortices stalled in the flight path. The LES results, however, illustrate that (i) the primary rectilinear vortices are rapidly deformed on the scale of the alternating updraft and downdraft regions; (ii) parts of the vortices stay on flight level but are quickly eroded by the enhanced turbulence of an updraft; (iii) longest living sections of the vortices are found in regions of relatively calm downdraft flow which augments their descent. Strip theory calculations are used to illustrate the temporal and spatial development of lift and rolling moments experienced by a following medium weight class B-737 aircraft. Characteristics of the respective distributions are analysed. Initially, the maximum rolling moments slightly exceed the available roll control of the B-737. After 60 seconds the probability of rolling moments exceeding 50% of the roll control, a value which is considered as a threshold for acceptable rolling moments, has decreased to 1% of its initial probability. (orig.)

  13. Glitches and pinned vorticity in the Crab pulsar

    Energy Technology Data Exchange (ETDEWEB)

    Alpar, M.A. (Bogazici Univ., Istanbul, Turkey); Anderson, P.W.; Pines, D.; Shaham, J.


    It is suggested that the glitch behavior observed in the Crab pulsar is associated with vorticity jumps induced by a starquake or a comparable external fluctuation in the weakly pinned vortex region expected in the crust of a young neutron star, and that the differences in the glitch behavior of the Crab, Vela, and older pulsars may be explained on evolutionary grounds.

  14. Numerical and Experimental Study of Electromagnetically Driven Vortical Flows

    NARCIS (Netherlands)

    Kenjeres, S.; Verdoold, J.; Tummers, M.J.; Hanjalic, K.; Kleijn, C.R.


    The paper reports on numerical and experimental investigations of electromagnetically driven vortical flows of an electrically conductive fluid in a generic setup. Two different configurations of permanent magnets are considered: a 3-magnet configuration in which the resulting Lorentz force is

  15. Turbulent transports by secondary flow vortices in a rod bundle

    Energy Technology Data Exchange (ETDEWEB)

    Vonka, V.


    Experimental data on secondary flow vortices in a rod bundle with pitch-to-diameter ratio P/D=1.3 show weak vortices with the average velocity magnitude only about 0.1% of the mean bulk velocity. The question then arises, how important these weak vortices could still be as a transport mechanism in turbulent flows. The transport of axial momentum by these vortices is analysed quantitatively. While a minor importance is observed for the transport in radial direction, it is found that about half of the total transport in circumferential direction is due to the secondary flow vortex convection. Based on the analogy between the transport of momentum and heat, it is expected in nonisothermal situations that, in radial direction, the contribution can improve the heat transfer coefficient and contribute to better economy of heat transfer installations. In circumferential direction, the contribution helps to smooth out circumferential temperature differences, improves the heat removal from heated surfaces and, through a decrease of the maximum surface temperature, it contributes to passive safety of heat transfer installations.

  16. Directed motion of vortices in faceted Nb films

    CERN Document Server

    Soroka, A K


    Guided motion of vortices in the Nb films on a faceted Al sub 2 O sub 3 substrate is observed. Even and odd components of longitudinal and transverse magnetoresistivities of the samples with different orientation of transport current to the facet ridges are measured. The field inversion was used to separate the even and odd components of the measured magnetoresistivities.

  17. Stability of Geostrophic Vortices on a Rotating Sphere (United States)


    equal strengh \\i equally spaced on a circle of colatitude 6, one vortex with strength u« at the pole; (c) the same distribution as in (a) but...N+l Vortices, No Boundary Condition at Equator. The formulas (2.1), (2.2) are inserted into (1.3), (1.4), (1.5). By expanding in powers of C

  18. Application of a mean vorticity covariance turbulence closure to flow past the aft part of an axisymmetric body (United States)

    Dai, Charles Mun Hong

    The Mean Vorticity Covariance (MVC) turbulence closure is derived with consideration of the relationship between Lagrangian and Eulerian statistics. Lagrangian correlation is shown to be defined in terms of a domain integral over a set of Eulerian correlations which are obtained concurrently at all positions in the flow field of interest. It is further demonstrated that a Langrangian correlation and an Eulerian correlation are equivalent in the case of homogeneous turbulence which forms the base for the MVC closure. A physical model which is based upon the simplifications made on the kinematics of the fluctuating part of the turbulent flow is proposed for the two-dimensional or the axisymmetric turbulent flow. A fully implicit finite difference technique is developed for the governing mean vorticity and covariance equations. The stream function equation is solved iteratively with the coupled equations.

  19. Potential vorticity of the south polar vortex of Venus (United States)

    Garate-Lopez, I.; Hueso, R.; Sánchez-Lavega, A.; García Muñoz, A.


    Venus' atmosphere shows highly variable warm vortices over both of the planet's poles. The nature of the mechanism behind their formation and properties is still unknown. Potential vorticity is a conserved quantity when advective processes dominate over friction and diabatic heating and is a quantity frequently used to model balanced flows. As a step toward understanding the vortices' dynamics, we present maps of Ertel's potential vorticity (EPV) at Venus' south polar region. We analyze three configurations of the south polar vortex at the upper cloud level (P ~ 240 mbar; z ~ 58 km), based on our previous analyses of cloud motions and thermal structure from data acquired by the Visual and InfraRed Thermal Imaging Spectrometer instrument on board Venus Express. Additionally, we tentatively estimate EPV at the lower cloud level (P ~ 2200 mbar; z ~ 43 km), based on our previous wind measurements and on static stability data from Pioneer Venus and the Venus International Reference Atmosphere (VIRA) model. Values of EPV are on the order of 10-6 and 10-8 K m2 kg-1 s-1 at the upper and lower cloud levels, respectively, being 3 times larger than the estimated errors. The morphology observed in EPV maps is mainly determined by the structures of the vertical component of the relative vorticity. This is in contrast to the vortex's morphology observed in 3.8 or 5 µm images which are related to the thermal structure of the atmosphere at the cloud top. Some of the EPV maps point to a weak ringed structure in the upper cloud, while a more homogenous EPV field is found in the lower cloud.

  20. Spatial-temporal and modal analysis of propeller induced ground vortices by particle image velocimetry

    NARCIS (Netherlands)

    Yang, Y.; Sciacchitano, A.; Veldhuis, L.L.M.; Eitelberg, G.


    During the ground operation of aircraft, there is potentially a system of vortices generated from the ground toward the propulsor, commonly denoted as ground vortices. Although extensive research has been conducted on ground vortices induced by turbofans which were simplified by suction tubes, these

  1. Vector Galileon and inflationary magnetogenesis (United States)

    Nandi, Debottam; Shankaranarayanan, S.


    Cosmological inflation provides the initial conditions for the structure formation. However, the origin of large-scale magnetic fields can not be addressed in this framework. The key issue for this long-standing problem is the conformal invariance of the electromagnetic (EM) field in 4-D. While many approaches have been proposed in the literature for breaking conformal invariance of the EM action, here, we provide a completely new way of looking at the modifications to the EM action and generation of primordial magnetic fields during inflation. We explicitly construct a higher derivative EM action that breaks conformal invariance by demanding three conditions—theory be described by vector potential Aμ and its derivatives, Gauge invariance be satisfied, and equations of motion be linear in second derivatives of vector potential. The unique feature of our model is that appreciable magnetic fields are generated at small wavelengths while tiny magnetic fields are generated at large wavelengths that are consistent with current observations.

  2. The Gravity of Dark Vortices: Effective Field Theory for Branes and Strings Carrying Localized Flux

    CERN Document Server

    Burgess, C P; Williams, M


    A Nielsen-Olesen vortex usually sits in an environment that expels the flux that is confined to the vortex, so flux is not present both inside and outside. We construct vortices for which this is not true, where the flux carried by the vortex also permeates the `bulk' far from the vortex. The idea is to mix the vortex's internal gauge flux with an external flux using off-diagonal kinetic mixing. Such `dark' vortices could play a phenomenological role in models with both cosmic strings and a dark gauge sector. When coupled to gravity they also provide explicit ultra-violet completions for codimension-two brane-localized flux, which arises in extra-dimensional models when the same flux that stabilizes extra-dimensional size is also localized on space-filling branes situated around the extra dimensions. We derive simple formulae for observables such as defect angle, tension, localized flux and on-vortex curvature when coupled to gravity, and show how all of these are insensitive to much of the microscopic detail...

  3. The Prospects for Future Satellite Mapping of Mesoscale and Submesoscale Vorticity (United States)

    Chelton, D. B., Jr.


    Presently available satellite data are limited to investigation of mesoscale variability with wavelength scales longer than about 200 km. Two future satellite missions offer promising prospects for observations of smaller-scale variability. One of these is the Surface Water/Ocean Topography (SWOT) altimeter mission with a planned launch in 2021. The other, which is in the early planning stages, is a radar scatterometer mission to measure surface winds and surface ocean currents, tentatively called the Winds and Currents Mission (WaCM). WaCM determines vector surface currents from the Doppler shift of the radar returns from multiple look angles. The resolution capabilities of vorticity fields constructed from measurements by these satellite instruments depend on both measurement errors and the sampling errors that arise from the limited swath widths and discrete sampling at the irregular times of satellite overflights. The effects of measurement and sampling errors are investigated separately and in combination from simulated SWOT and WaCM sampling of, respectively, the SSH and surface velocity fields in a high-resolution (0.5 km grid spacing) numerical model of the California Current System. Because of the narrow swath width of 120 km with a 20 km nadir gap for SWOT, the resolution capability of SWOT data is limited primarily by sampling errors. Since WaCM has a much wider swath width of 1300 km with a 100 km nadir gap, the resolution capability of WaCM data is limited primarily by measurement errors. For the present baseline measurement accuracies of the two instruments, the resolution capability of vorticity fields constructed from WaCM data is significantly better than that from SWOT data. Moreover, WaCM offers dramatic improvement over present capabilities for mapping mesoscale variability. Resolving submesoscale variability, however, will require better measurement accuracy than the current baseline.

  4. A coupled Eulerian/Lagrangian method for the solution of three-dimensional vortical flows (United States)

    Felici, Helene Marie


    A coupled Eulerian/Lagrangian method is presented for the reduction of numerical diffusion observed in solutions of three-dimensional rotational flows using standard Eulerian finite-volume time-marching procedures. A Lagrangian particle tracking method using particle markers is added to the Eulerian time-marching procedure and provides a correction of the Eulerian solution. In turn, the Eulerian solutions is used to integrate the Lagrangian state-vector along the particles trajectories. The Lagrangian correction technique does not require any a-priori information on the structure or position of the vortical regions. While the Eulerian solution ensures the conservation of mass and sets the pressure field, the particle markers, used as 'accuracy boosters,' take advantage of the accurate convection description of the Lagrangian solution and enhance the vorticity and entropy capturing capabilities of standard Eulerian finite-volume methods. The combined solution procedures is tested in several applications. The convection of a Lamb vortex in a straight channel is used as an unsteady compressible flow preservation test case. The other test cases concern steady incompressible flow calculations and include the preservation of turbulent inlet velocity profile, the swirling flow in a pipe, and the constant stagnation pressure flow and secondary flow calculations in bends. The last application deals with the external flow past a wing with emphasis on the trailing vortex solution. The improvement due to the addition of the Lagrangian correction technique is measured by comparison with analytical solutions when available or with Eulerian solutions on finer grids. The use of the combined Eulerian/Lagrangian scheme results in substantially lower grid resolution requirements than the standard Eulerian scheme for a given solution accuracy.

  5. Effect of Intraseasonal Oscillation on the Vortices Moving off the Tibetan Plateau (United States)

    Zhang, R.; Li, L.


    In the present study, the relationship between the atmospheric intraseasonal oscillation (ISO) and Tibetan Plateau vortices (TPVs) moving off the Tibetan Plateau during May-August of 1998-2012 was investigated based on the radiosonde and reanalysis data. It is found that the number of TPVs moving off the Tibetan Plateau have the distinct feature of the 10-20-day ISO. 77% of the moving-off TPVs occur in the positive phases of the 10-20-day filtered 500 hPa vorticity over eastern Tibetan Plateau. Besides, distributions of the zonal and meridional components of E-vector coincide well with trajectories of the TPVs, indicating the important effect of the propagation of the ISO energy on the movement of the TPVs. The atmospheric circulations and related thermodynamic effects are discussed to reveal the mechanism of the effect of 10-20-day ISO on the moving-off TPVs. It is found that the interaction between atmospheric circulations and heating field of 10-20-day ISO has major impact on the moving-off TPVs. In positive ISO phases, at 500 hPa over eastern plateau, there appear negative geopotential height anomalies and anomalous cyclonic wind shear; the anomalous jet stream and positive geopotential height at 200 hPa lying over the northeast of the Tibetan Plateau stretch eastward gradually, benefiting for the upper level divergence and ascending motion. The rainfall is triggered under this situation, and the condensation latent heat is released and shifts eastward with the heating centers located at 400 hPa, which depress the isobaric surface at 500 hPa. All these conditions are in favor of the maintenance and eastward movement of the TPVs.

  6. Growth and wall-transpiration control of nonlinear unsteady Görtler vortices forced by free-stream vortical disturbances (United States)

    Marensi, Elena; Ricco, Pierre


    The generation, nonlinear evolution, and wall-transpiration control of unsteady Görtler vortices in an incompressible boundary layer over a concave plate is studied theoretically and numerically. Görtler rolls are initiated and driven by free-stream vortical perturbations of which only the low-frequency components are considered because they penetrate the most into the boundary layer. The formation and development of the disturbances are governed by the nonlinear unsteady boundary-region equations with the centrifugal force included. These equations are subject to appropriate initial and outer boundary conditions, which account for the influence of the upstream and free-stream forcing in a rigorous and mutually consistent manner. Numerical solutions show that the stabilizing effect on nonlinearity, which also occurs in flat-plate boundary layers, is significantly enhanced in the presence of centrifugal forces. Sufficiently downstream, the nonlinear vortices excited at different free-stream turbulence intensities Tu saturate at the same level, proving that the initial amplitude of the forcing becomes unimportant. At low Tu, the disturbance exhibits a quasi-exponential growth with the growth rate being intensified for more curved plates and for lower frequencies. At higher Tu, in the typical range of turbomachinery applications, the Görtler vortices do not undergo a modal stage as nonlinearity saturates rapidly, and the wall curvature does not affect the boundary-layer response. Good quantitative agreement with data from direct numerical simulations and experiments is obtained. Steady spanwise-uniform and spanwise-modulated zero-mass-flow-rate wall transpiration is shown to attenuate the growth of the Görtler vortices significantly. A novel modified version of the Fukagata-Iwamoto-Kasagi identity, used for the first time to study a transitional flow, reveals which terms in the streamwise momentum balance are mostly affected by the wall transpiration, thus

  7. Vorticity and upscaled dispersion in 3D heterogeneous porous media (United States)

    Di Dato, Mariaines; Chiogna, Gabriele; de Barros, Felipe; Bellin, Alberto; Fiori, Aldo


    Modeling flow in porous media is relevant for many environmental, energy and industrial applications. From an environmental perspective, the relevance of porous media flow becomes evident in subsurface hydrology. In general, flow in natural porous media is creeping, yet the large variability in the hydraulic conductivity values encountered in natural aquifers leads to highly heterogeneous flow fields. This natural variability in the conductivity field will affect both dilution rates of chemical species and reactive mixing. A physical consequence of this heterogeneity is also the presence of a various localized kinematical features such as straining, shearing and vorticity in aquifers, which will influence the shape of solute clouds and its fate and transport. This work aims in fundamentally characterizing the vorticity field in spatially heterogeneous flow fields as a function of their statistical properties in order to analyze the impact on transport processes. In our study, three-dimensional porous formations are constructed with an ensemble of N independent, non-overlapping spheroidal inclusions submerged into an homogeneous matrix, of conductivity K0. The inclusions are randomly located in a domain of volume W and are fully characterized by the geometry of spheroid (oblate or prolate), their conductivity K (random and drawn from a given probability density function fκ), the centroid location ¯x, the axes ratio e, the orientation of the rotational axis (α1,α2) and the volume w. Under the assumption of diluted medium, the flow problem is solved analitically by means of only two parameters: the conductivity contrast κ = K/K0 and the volume fraction n = Nw/W . Through the variation of these parameters of the problem, it is possible to approximate the structure of natural heterogeneous porous media. Using a random distribution of the orientation of the inclusions, we create media defined by the same global anisotropy f = Iz/Ix but different micro

  8. Vortices and other topological solitons in dense quark matter (United States)

    Eto, Minoru; Hirono, Yuji; Nitta, Muneto; Yasui, Shigehiro


    Dense quantum chromodynamic matter accommodates various kind of topological solitons such as vortices, domain walls, monopoles, kinks, boojums, and so on. In this review, we discuss various properties of topological solitons in dense quantum chromodynamics (QCD) and their phenomenological implications. Particular emphasis is placed on the topological solitons in the color-flavor-locked (CFL) phase, which exhibits both superfluidity and superconductivity. The properties of topological solitons are discussed in terms of effective field theories such as the Ginzburg-Landau theory, the chiral Lagrangian, or the Bogoliubov-de Gennes equation. The most fundamental string-like topological excitations in the CFL phase are non-Abelian vortices, which are 1/3 quantized superfluid vortices and color magnetic flux tubes. These vortices are created at a phase transition by the Kibble-Zurek mechanism or when the CFL phase is realized in compact stars, which rotate rapidly. The interaction between vortices is found to be repulsive and consequently a vortex lattice is formed in rotating CFL matter. Bosonic and fermionic zero-energy modes are trapped in the core of a non-Abelian vortex and propagate along it as gapless excitations. The former consists of translational zero modes (a Kelvin mode) with a quadratic dispersion and {mathbb {C}}P^2 Nambu-Goldstone gapless modes with a linear dispersion, associated with the CFL symmetry spontaneously broken in the core of a vortex, while the latter is Majorana fermion zero modes belonging to the triplet of the symmetry remaining in the core of a vortex. The low-energy effective theory of the bosonic zero modes is constructed as a non-relativistic free complex scalar field and a relativistic {mathbb {C}}P^2 model in 1+1 dimensions. The effects of strange quark mass, electromagnetic interactions, and non-perturbative quantum corrections are taken into account in the {mathbb {C}}P^2 effective theory. Various topological objects associated

  9. [Protective effect of adeno-associated viral vector-mediated expression of human brain-derived neurotrophic factor in rat neurons against beta-amyloid-induced Alzheimer's disease in vitro]. (United States)

    Liu, Zhao-hui; Ma, Dong-liang; Jin, Hui; Ma, Yan-bing; Hu, Hai-tao


    To achieve expression of human brain-derived neurotrophic factor (hBDNF) mediated by recombinant adeno-associated virus (rAAV) and explore the mechanism of its neuroprotective effects in rat neurons against beta-amyloid-induced Alzheimer's disease. Using molecular cloning technique, rAAV vector containing hBDNF gene (AAV-hBDNF) was constructed to transfect SD rat hippocampal neurons exposed to beta-amyloid treatment. The changes in cell apoptosis were observed by MTT assay and flow cytometry, and the expression of hBDNF and Bcl-2 protein were determined by immunocytochemical staining. Laser scanning confocal microscopy (LSCM) was used to observe the changes of [Ca(2+)](i). The cultured rat hippocampal neurons were effectively transfected with AAV-hBDNF and expression of BDNF protein was obviously increased. hBNDF expression showed significant protective effects against beta-amyloid-induced neuronal damage, and the expression of Bcl-2 protein was increased significantly and the balance of [Ca(2+)](i) was maintained in BDNF-treated cells with beta-amyloid exposure. hBDNF expression can effectively protect cultured rat hippocampal cells from beta-amyloid-induced apoptosis through inhibiting the intracellular calcium overload and increasing the expression of Bcl-2 protein.

  10. The gravity of dark vortices: effective field theory for branes and strings carrying localized flux

    Energy Technology Data Exchange (ETDEWEB)

    Burgess, C.P. [Physics & Astronomy, McMaster University, Hamilton, ON, L8S 4M1 (Canada); Perimeter Institute for Theoretical Physics, Waterloo, ON, N2L 2Y5 (Canada); Division PH -TH, CERN, CH-1211, Genève 23 (Switzerland); Diener, R. [Physics & Astronomy, McMaster University, Hamilton, ON, L8S 4M1 (Canada); Perimeter Institute for Theoretical Physics, Waterloo, ON, N2L 2Y5 (Canada); Williams, M. [Instituut voor Theoretische Fysica, Katholieke Universiteit Leuven, B-3001 Leuven (Belgium)


    A Nielsen-Olesen vortex usually sits in an environment that expels the flux that is confined to the vortex, so flux is not present both inside and outside. We construct vortices for which this is not true, where the flux carried by the vortex also permeates the ‘bulk’ far from the vortex. The idea is to mix the vortex’s internal gauge flux with an external flux using off-diagonal kinetic mixing. Such ‘dark’ vortices could play a phenomenological role in models with both cosmic strings and a dark gauge sector. When coupled to gravity they also provide explicit ultra-violet completions for codimension-two brane-localized flux, which arises in extra-dimensional models when the same flux that stabilizes extra-dimensional size is also localized on space-filling branes situated around the extra dimensions. We derive simple formulae for observables such as defect angle, tension, localized flux and on-vortex curvature when coupled to gravity, and show how all of these are insensitive to much of the microscopic details of the solutions, and are instead largely dictated by low-energy quantities. We derive the required effective description in terms of a world-sheet brane action, and derive the matching conditions for its couplings. We consider the case where the dimensions transverse to the bulk compactify, and determine how the on- and off-vortex curvatures and other bulk features depend on the vortex properties. We find that the brane-localized flux does not gravitate, but just renormalizes the tension in a magnetic-field independent way. The existence of an explicit UV completion puts the effective description of these models on a more precise footing, verifying that brane-localized flux can be consistent with sensible UV physics and resolving some apparent paradoxes that can arise with a naive (but commonly used) delta-function treatment of the brane’s localization within the bulk.

  11. The gravity of dark vortices: effective field theory for branes and strings carrying localized flux (United States)

    Burgess, C. P.; Diener, R.; Williams, M.


    A Nielsen-Olesen vortex usually sits in an environment that expels the flux that is confined to the vortex, so flux is not present both inside and outside. We construct vortices for which this is not true, where the flux carried by the vortex also permeates the `bulk' far from the vortex. The idea is to mix the vortex's internal gauge flux with an external flux using off-diagonal kinetic mixing. Such `dark' vortices could play a phenomenological role in models with both cosmic strings and a dark gauge sector. When coupled to gravity they also provide explicit ultra-violet completions for codimension-two brane-localized flux, which arises in extra-dimensional models when the same flux that stabilizes extra-dimensional size is also localized on space-filling branes situated around the extra dimensions. We derive simple formulae for observables such as defect angle, tension, localized flux and on-vortex curvature when coupled to gravity, and show how all of these are insensitive to much of the microscopic details of the solutions, and are instead largely dictated by low-energy quantities. We derive the required effective description in terms of a world-sheet brane action, and derive the matching conditions for its couplings. We consider the case where the dimensions transverse to the bulk compactify, and determine how the on- and off-vortex curvatures and other bulk features depend on the vortex properties. We find that the brane-localized flux does not gravitate, but just renormalizes the tension in a magnetic-field independent way. The existence of an explicit UV completion puts the effective description of these models on a more precise footing, verifying that brane-localized flux can be consistent with sensible UV physics and resolving some apparent paradoxes that can arise with a naive (but commonly used) delta-function treatment of the brane's localization within the bulk.

  12. Discretization of the vorticity field of a planar jet (United States)

    Ross, Natalie; Hertzberg, Jean; Bradley, Elizabeth


    In data assimilation, information from sensors is used to correct the state variables of a numerical model. This has been used to great advantage by the weather prediction community in the context of direct numerical simulation (DNS) models, but has seen comparatively little use in point-vortex models. This is due in large part to data-processing issues. In order to keep up with the speeds necessary for effective data assimilation, one must extract and discretize the vortex structures from velocity field data in a computationally efficient fashion—i.e., using as few discrete vortices as possible to model the measured flow. This paper describes a new strategy for accomplishing this and evaluates the results using data from a laboratory-scale vortex-dominated planar jet. Large-scale vortex structures are found using a family of variants on traditional vortex extraction methods. By augmenting these methods with simple computational topology techniques, one obtains a new method that finds the boundaries of the coherent structures in a manner that naturally follows the geometry of the flow. This strategy was evaluated in the context of two standard vortex extraction methods, vorticity thresholding and Okubo-Weiss, and tested upon velocity field data from the experimental fluid flow. The large-scale structures found in this manner were then modeled with collections of discrete vortices, and the effects of the grain size of the discretization and the parameters of the discrete vortex model were studied. The results were evaluated by comparing the instantaneous velocity field induced by the discrete vortices to that measured in the jet. These comparisons showed that the two extraction techniques were comparable in terms of sensitivity and error, suggesting that the computationally simpler vorticity thresholding method is more appropriate for applications where speed is an issue, like data assimilation. Comparisons of different discretization strategies showed that

  13. Spanwise Spacing Effects on the Initial Structure and Decay of Axial Vortices (United States)

    Wendt, B. J.; Reichert, B. A.


    The initial structure and axial decay of an array of streamwise vortices embedded in a turbulent pipe boundary layer is experimentally investigated. The vortices are shed in counter-rotating fashion from an array of equally-spaced symmetric airfoil vortex generators. Vortex structure is quantified in terms of crossplane circulation and peak streamwise vorticity. Flow conditions are subsonic and incompressible. The focus of this study is on the effect of the initial spacing between the parent vortex generators. Arrays with vortex generators spaced at 15 and 30 degrees apart are considered. When the spacing between vortex generators is decreased the circulation and peak vorticity of the shed vortices increases. Analysis indicates this strengthening results from regions of fluid acceleration in the vicinity of the vortex generator array. Decreased spacing between the constituent vortices also produces increased rates of circulation and peak vorticity decay.

  14. Numerical study of the evolution of vortices in a linearly stratified fluid

    Energy Technology Data Exchange (ETDEWEB)

    Beckers, M.; Clercx, H.J.H.; Van Heijst, G.J.F. [Eindhoven University of Technology, Eindhoven (Netherlands). J.M. Burgers Centre fro Fluid Dynamics; Verzicco, R. [Rome Univ. La Sapienza, Rome (Italy). Dipt. di Meccanica e Aeronautica


    This paper presents a numerical study in which the evolution of vortices in a Stafford fluid is compared to the evolution of two-dimensional vortices. The influence of the Reynolds number and the Froude number are investigated, for the evolution of axisymmetric vortices, for their azimuthal instability and for the subsequent formation of tripoles. It is found that due to radial diffusion axisymmetric vortices with various initial vorticity profiles all evolve towards the same profile. This evolution reduces the growth of azimuthal instabilities which may lead to the formation of a tripole. For vortices in a stratified fluid the effect of the ambient stratification on the evolution of the vortices is investigated. It is found that a process of vortex stretching, which becomes more pronounced for increasing Froude numbers, leads to a weaker tripole formation.

  15. Helicity and Vorticity of Pulmonary Arterial Flow in Patients With Pulmonary Hypertension: Quantitative Analysis of Flow Formations. (United States)

    Schäfer, Michal; Barker, Alex J; Kheyfets, Vitaly; Stenmark, Kurt R; Crapo, James; Yeager, Michael E; Truong, Uyen; Buckner, J Kern; Fenster, Brett E; Hunter, Kendall S


    Qualitative and quantitative flow hemodynamic indexes have been shown to reflect right ventricular (RV) afterload and function in pulmonary hypertension (PH). We aimed to quantify flow hemodynamic formations in pulmonary arteries using 4-dimensional flow cardiac magnetic resonance imaging and the spatial velocity derivatives helicity and vorticity in a heterogeneous PH population. Patients with PH (n=35) and controls (n=10) underwent 4-dimensional flow magnetic resonance imaging study for computation of helicity and vorticity in the main pulmonary artery (MPA), the right pulmonary artery, and the RV outflow tract. Helicity and vorticity were correlated with standard RV volumetric and functional indexes along with MPA stiffness assessed by measuring relative area change. Patients with PH had a significantly decreased helicity in the MPA (8 versus 32 m/s2; Pflow hemodynamic character in patients with PH assessed via quantitative analysis is considerably different when compared with healthy and normotensive controls. A strong association between helicity in pulmonary arteries and ventricular-vascular coupling suggests a relationship between the mechanical and flow hemodynamic domains. © 2017 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley.

  16. Fractional vortices in the XY model with {pi} bonds

    Energy Technology Data Exchange (ETDEWEB)

    Kulkarni, R. V.; Almaas, E.; Fisher, K. D.; Stroud, D.


    We define a new set of excitations in the XY model which we call ''fractional vortices.'' In the frustrated XY model containing {pi} bonds, we make the ansatz that the ground state configurations can be characterized by pairs of oppositely charged fractional vortices. For a chain of {pi} bonds, the ground state energy and the phase configurations calculated on the basis of this ansatz agree well with the results from direct numerical simulations. Finally, we discuss the possible connection of these results to some recent experiments by Kirtley [Phys. Rev. B 51, R12057 (1995)] on high-T{sub c} superconductors where fractional flux trapping was observed along certain grain boundaries.

  17. Neutrino induced vorticity, Alfven waves and the normal modes

    Energy Technology Data Exchange (ETDEWEB)

    Bhatt, Jitesh R. [Theory Division, Physical Research Laboratory, Ahmedabad (India); George, Manu [Theory Division, Physical Research Laboratory, Ahmedabad (India); Indian Institute of Technology, Department of Physics, Ahmedabad (India)


    We consider a plasma consisting of electrons and ions in the presence of a background neutrino gas and develop the magnetohydrodynamic equations for the system. We show that the electron neutrino interaction can induce vorticity in the plasma even in the absence of any electromagnetic perturbations if the background neutrino density is left-right asymmetric. This induced vorticity supports a new kind of Alfven wave whose velocity depends on both the external magnetic field and on the neutrino asymmetry. The normal mode analysis show that in the presence of neutrino background the Alfven waves can have different velocities. We also discuss our results in the context of dense astrophysical plasma such as magnetars and show that the difference in the Alfven velocities can be used to explain the observed pulsar kick. We discuss also the relativistic generalisation of the electron fluid in presence of an asymmetric neutrino background. (orig.)

  18. Formation of optical vortices through superposition of two Gaussian beams. (United States)

    Vaity, Pravin; Aadhi, A; Singh, R P


    We observe phase singularities in the superposed field of two Gaussian beams. It is seen that the formation of these singularities depends on the tilt between two Gaussian beams and the separation of their beam axes. By reversing the angle or the position of the beams, one can change the sign of the vortex. We have shown the formation of single as well as multiple vortices by changing the tilt angle and the position of two Gaussian beams. The experimental results are verified with theoretical analysis. We also observe that such a vortex structure can be formed through superposition of two backreflected Gaussian beams from any optical element with two flat surfaces, as illustrated through a beam splitter and a neutral density filter. This technique is very useful for generation of vortices with high-power lasers where one cannot use a spatial light modulator.

  19. Measurement of secondary flow vortices on a rod bundle

    Energy Technology Data Exchange (ETDEWEB)

    Vonka, V.


    Secondary flow vortices in infinite rod bundles have been predicted by a number of theoretical analyses. Nevertheless experimental verification was difficult, since the magnitude of the secondary velocities appeared to be less than the accuracy of the experimental techniques used. Only indications of the maximum velocity magnitude have been available but no report on successful direct measurement is known to the author. At ECN, laser Doppler velocimetry is successfully used for measurement of secondary flow vortices in two regular subchannels of a triangularly arranged bare rod bundle with pitch-to-diameter ratio P/D-1.3 under the Reynolds number conditions 60,000 and 175,000. One single secondary vortex, having the average tangential velocity slightly less than 0.1% of the mean bulk velocity, is resolved per minimum symmetry sector of the bundle geometry. Ensemble averages are made to obtain quantitative description of the vortex and to form a data base for comparison with calculations.

  20. Mixing and coherent vortices in turbulent coaxial jets (United States)

    Balarac, Guillaume; Si-Ameur, Mohamed


    Direct numerical simulations associated with mixing in constant-density round coaxial jets are performed. They are validated by comparison against laboratory experiments. The mixing process is studied by seeding a passive tracer first in the outer annular jet, then in the inner jet. We demonstrate the important role played by coherent vortices in the mixing mechanisms. The turbulent mixing exhibits an intermittent character as a consequence of fluid ejections caused by the counter-rotating streamwise vortices. We quantify also the domination of the outer jet and show that the fluid issuing from the central jet remains confined. To cite this article: G. Balarac, M. Si-Ameur, C. R. Mecanique 333 (2005).

  1. Helicity and topology of a small region of quantum vorticity (United States)

    Mesgarnezhad, M.; Cooper, R. G.; Baggaley, A. W.; Barenghi, C. F.


    We numerically study the evolution of a small turbulent region of quantised vorticity in superfluid helium, a regime which can be realised in the laboratory. We show that the turbulence achieves a fluctuating steady-state in terms of dynamics (energy), geometry (length, writhing) and topology (linking). We show that, at any instant, the turbulence consists of many unknots and few large loops of great geometrical and topological complexity.

  2. Structure and evolution of 3D Rossby Vortices (United States)

    Richard, S.; Barge, P.


    Three dimensional compressible simulations of the Rossby Wave Instability are presented in a non-homentropic model of protoplanetary disk. The instability develops like in the two dimensional case, gradually coming to the formation of a single big vortex. This 3D vortex has a quasi-2D structure which looks like a vorticity column with only tiny vertical motions. The vortex survives hundred of rotations in a quasi-steady evolution and slowly migrates inward toward the star.

  3. Vortical ciliary flows actively enhance mass transport in reef corals. (United States)

    Shapiro, Orr H; Fernandez, Vicente I; Garren, Melissa; Guasto, Jeffrey S; Debaillon-Vesque, François P; Kramarsky-Winter, Esti; Vardi, Assaf; Stocker, Roman


    The exchange of nutrients and dissolved gasses between corals and their environment is a critical determinant of the growth of coral colonies and the productivity of coral reefs. To date, this exchange has been assumed to be limited by molecular diffusion through an unstirred boundary layer extending 1-2 mm from the coral surface, with corals relying solely on external flow to overcome this limitation. Here, we present direct microscopic evidence that, instead, corals can actively enhance mass transport through strong vortical flows driven by motile epidermal cilia covering their entire surface. Ciliary beating produces quasi-steady arrays of counterrotating vortices that vigorously stir a layer of water extending up to 2 mm from the coral surface. We show that, under low ambient flow velocities, these vortices, rather than molecular diffusion, control the exchange of nutrients and oxygen between the coral and its environment, enhancing mass transfer rates by up to 400%. This ability of corals to stir their boundary layer changes the way that we perceive the microenvironment of coral surfaces, revealing an active mechanism complementing the passive enhancement of transport by ambient flow. These findings extend our understanding of mass transport processes in reef corals and may shed new light on the evolutionary success of corals and coral reefs.

  4. Mechanics of Individual, Isolated Vortices in a Cuprate Superconductor

    Energy Technology Data Exchange (ETDEWEB)

    Auslaender, M.


    Superconductors often contain quantized microscopic whirlpools of electrons, called vortices, that can be modeled as one-dimensional elastic objects. Vortices are a diverse playground for condensed matter because of the interplay between thermal fluctuations, vortex-vortex interactions, and the interaction of the vortex core with the three-dimensional disorder landscape. While vortex matter has been studied extensively, the static and dynamic properties of an individual vortex have not. Here we employ magnetic force microscopy (MFM) to image and manipulate individual vortices in detwinned, single crystal YBa{sub 2}Cu{sub 3}O{sub 6.991} (YBCO), directly measuring the interaction of a moving vortex with the local disorder potential. We find an unexpected and dramatic enhancement of the response of a vortex to pulling when we wiggle it transversely. In addition, we find enhanced vortex pinning anisotropy that suggests clustering of oxygen vacancies in our sample and demonstrates the power of MFM to probe vortex structure and microscopic defects that cause pinning.

  5. Heat-Transfer Enhancement by Artificially Generated Streamwise Vorticity (United States)

    Ghanem, Akram; Habchi, Charbel; Lemenand, Thierry; Della Valle, Dominique; Peerhossaini, Hassan


    Vortex-induced heat transfer enhancement exploits longitudinal and transverse pressure-driven vortices through the deliberate artificial generation of large-scale vortical flow structures. Thermal-hydraulic performance, Nusselt number and friction factor are experimentally investigated in a HEV (high-efficiency vortex) mixer, which is a tubular heat exchanger and static mixer equipped with trapezoidal vortex generators. Pressure gradients are generated on the trapezoidal tab initiating a streamwise swirling motion in the form of two longitudinal counter-rotating vortex pairs (CVP). Due to the Kelvin-Helmholtz instability, the shear layer generated at the tab edges, which is a production site of turbulence kinetic energy (TKE), becomes unstable further downstream from the tabs and gives rise to periodic hairpin vortices. The aim of the study is to quantify the effects of hydrodynamics on the heat- and masstransfer phenomena accompanying such flows for comparison with the results of numerical studies and validate the high efficiency of the intensification process implementing such vortex generators. The experimental results reflect the enhancement expected from the numerical studies and confirm the high status of the HEV heat exchanger and static mixer.

  6. Lagrangian structures in time-periodic vortical flows

    Directory of Open Access Journals (Sweden)

    S. V. Kostrykin


    Full Text Available The Lagrangian trajectories of fluid particles are experimentally studied in an oscillating four-vortex velocity field. The oscillations occur due to a loss of stability of a steady flow and result in a regular reclosure of streamlines between the vortices of the same sign. The Eulerian velocity field is visualized by tracer displacements over a short time period. The obtained data on tracer motions during a number of oscillation periods show that the Lagrangian trajectories form quasi-regular structures. The destruction of these structures is determined by two characteristic time scales: the tracers are redistributed sufficiently fast between the vortices of the same sign and much more slowly transported into the vortices of opposite sign. The observed behavior of the Lagrangian trajectories is quantitatively reproduced in a new numerical experiment with two-dimensional model of the velocity field with a small number of spatial harmonics. A qualitative interpretation of phenomena observed on the basis of the theory of adiabatic chaos in the Hamiltonian systems is given. The Lagrangian trajectories are numerically simulated under varying flow parameters. It is shown that the spatial-temporal characteristics of the Lagrangian structures depend on the properties of temporal change in the streamlines topology and on the adiabatic parameter corresponding to the flow. The condition for the occurrence of traps (the regions where the Lagrangian particles reside for a long time is obtained.

  7. Generation of optical vortices in an integrated optical circuit (United States)

    Tudor, Rebeca; Kusko, Mihai; Kusko, Cristian


    In this work, the generation of optical vortices in an optical integrated circuit is numerically demonstrated. The optical vortices with topological charge m = ±1 are obtained by the coherent superposition of the first order modes present in a waveguide with a rectangular cross section, where the phase delay between these two propagating modes is Δφ = ±π/2. The optical integrated circuit consists of an input waveguide continued with a y-splitter. The left and the right arms of the splitter form two coupling regions K1 and K2 with a multimode output waveguide. In each coupling region, the fundamental modes present in the arms of the splitter are selectively coupled into the output waveguide horizontal and vertical first order modes, respectively. We showed by employing the beam propagation method simulations that the fine tuning of the geometrical parameters of the optical circuit makes possible the generation of optical vortices in both transverse electric (TE) and transverse magnetic (TM) modes. Also, we demonstrated that by placing a thermo-optical element on one of the y-splitter arms, it is possible to switch the topological charge of the generated vortex from m = 1 to m = ‑1.

  8. Extreme-Ultraviolet Vortices from a Free-Electron Laser

    Directory of Open Access Journals (Sweden)

    Primož Rebernik Ribič


    Full Text Available Extreme-ultraviolet vortices may be exploited to steer the magnetic properties of nanoparticles, increase the resolution in microscopy, and gain insight into local symmetry and chirality of a material; they might even be used to increase the bandwidth in long-distance space communications. However, in contrast to the generation of vortex beams in the infrared and visible spectral regions, production of intense, extreme-ultraviolet and x-ray optical vortices still remains a challenge. Here, we present an in-situ and an ex-situ technique for generating intense, femtosecond, coherent optical vortices at a free-electron laser in the extreme ultraviolet. The first method takes advantage of nonlinear harmonic generation in a helical undulator, producing vortex beams at the second harmonic without the need for additional optical elements, while the latter one relies on the use of a spiral zone plate to generate a focused, micron-size optical vortex with a peak intensity approaching 10^{14}  W/cm^{2}, paving the way to nonlinear optical experiments with vortex beams at short wavelengths.

  9. The impact of intraglottal vortices on vocal fold dynamics (United States)

    Erath, Byron; Pirnia, Alireza; Peterson, Sean


    During voiced speech a critical pressure is produced in the lungs that separates the vocal folds and creates a passage (the glottis) for airflow. As air passes through the vocal folds the resulting aerodynamic loading, coupled with the tissue properties of the vocal folds, produces self-sustained oscillations. Throughout each cycle a complex flow field develops, characterized by a plethora of viscous flow phenomena. Air passing through the glottis creates a jet, with periodically-shed vortices developing due to flow separation and the Kelvin-Helmholtz instability in the shear layer. These vortices have been hypothesized to be a crucial mechanism for producing vocal fold vibrations. In this study the effect of vortices on the vocal fold dynamics is investigated experimentally by passing a vortex ring over a flexible beam with the same non-dimensional mechanical properties as the vocal folds. Synchronized particle image velocimetry data are acquired in tandem with the beam dynamics. The resulting impact of the vortex ring loading on vocal fold dynamics is discussed in detail. This work was supported by the National Science Foundation Grant CBET #1511761.

  10. Machine learning vortices at the Kosterlitz-Thouless transition (United States)

    Beach, Matthew J. S.; Golubeva, Anna; Melko, Roger G.


    Efficient and automated classification of phases from minimally processed data is one goal of machine learning in condensed-matter and statistical physics. Supervised algorithms trained on raw samples of microstates can successfully detect conventional phase transitions via learning a bulk feature such as an order parameter. In this paper, we investigate whether neural networks can learn to classify phases based on topological defects. We address this question on the two-dimensional classical XY model which exhibits a Kosterlitz-Thouless transition. We find significant feature engineering of the raw spin states is required to convincingly claim that features of the vortex configurations are responsible for learning the transition temperature. We further show a single-layer network does not correctly classify the phases of the XY model, while a convolutional network easily performs classification by learning the global magnetization. Finally, we design a deep network capable of learning vortices without feature engineering. We demonstrate the detection of vortices does not necessarily result in the best classification accuracy, especially for lattices of less than approximately 1000 spins. For larger systems, it remains a difficult task to learn vortices.

  11. Extreme-Ultraviolet Vortices from a Free-Electron Laser (United States)

    Rebernik Ribič, Primož; Rösner, Benedikt; Gauthier, David; Allaria, Enrico; Döring, Florian; Foglia, Laura; Giannessi, Luca; Mahne, Nicola; Manfredda, Michele; Masciovecchio, Claudio; Mincigrucci, Riccardo; Mirian, Najmeh; Principi, Emiliano; Roussel, Eléonore; Simoncig, Alberto; Spampinati, Simone; David, Christian; De Ninno, Giovanni


    Extreme-ultraviolet vortices may be exploited to steer the magnetic properties of nanoparticles, increase the resolution in microscopy, and gain insight into local symmetry and chirality of a material; they might even be used to increase the bandwidth in long-distance space communications. However, in contrast to the generation of vortex beams in the infrared and visible spectral regions, production of intense, extreme-ultraviolet and x-ray optical vortices still remains a challenge. Here, we present an in-situ and an ex-situ technique for generating intense, femtosecond, coherent optical vortices at a free-electron laser in the extreme ultraviolet. The first method takes advantage of nonlinear harmonic generation in a helical undulator, producing vortex beams at the second harmonic without the need for additional optical elements, while the latter one relies on the use of a spiral zone plate to generate a focused, micron-size optical vortex with a peak intensity approaching 1014 W /cm2 , paving the way to nonlinear optical experiments with vortex beams at short wavelengths.

  12. Some exact BPS solutions for exotic vortices and monopoles

    Directory of Open Access Journals (Sweden)

    Handhika S. Ramadhan


    Full Text Available We present several analytical solutions of BPS vortices and monopoles in the generalized Abelian Maxwell–Higgs and Yang–Mills–Higgs theories, respectively. These models have recently been extensively studied and several exact solutions have already been obtained in [1,2]. In each theory, the dynamics is controlled by the additional two positive scalar-field-dependent functions, f(|ϕ| and w(|ϕ|. For the case of vortices, we work in the ordinary symmetry-breaking Higgs potential, while for the case of monopoles we have the ordinary condition of the Prasad–Sommerfield limit. Our results generalize the exact solutions found previously. We also present solutions for BPS vortices with higher winding number. These solutions suffer from the condition that w(|ϕ| has negative value at some finite range of r, but we argue that since it satisfies the weaker positive-value conditions then the corresponding energy density is still positive-definite and, thus, they are acceptable BPS solutions.

  13. Characterization of Surfaces and the Estimation of Shock Induced Vorticity

    Energy Technology Data Exchange (ETDEWEB)

    Jameson, L; Ray, J; Peyser, T


    When shocks impinge on a surface separating fluids of two different densities, one observes the development and growth of various vortical structures. The flow induced by this Richtmyer-Meshkov (RM) instability depends on a variety of factors such as the shock strength, the density ratio of the fluids and the exact form of the surface perturbation. The most common way to model the form of the surface perturbation is through Fourier analysis which is suitable for large-scale sinusoidal structures and is straightforward mathematically. In surfaces of practical interest, however, to a wide range of application, there may also be a broad spectrum of high frequency modes in addition to the lower frequency modes described by Fourier methods. We propose here that these high frequency modes can be efficiently quantified in terms of wavelet analysis. From a numerical point of view, the scale that the roughness occurs at is far to small to numerically resolve and thus we propose that our new methodology can be used to model the subgrid scale generation of vorticity. Thus the combination of wavelet analysis and Fourier analysis will be used to model the generation of vorticity for the RM instability.

  14. Hilbert Statistics of Vorticity Scaling in Two-Dimensional Turbulence

    CERN Document Server

    Tan, H S; Meng, Jianping


    In this paper, the scaling property of the inverse energy cascade and forward enstrophy cascade of the vorticity filed $\\omega(x,y)$ in two-dimensional (2D) turbulence is analyzed. This is accomplished by applying a Hilbert-based technique, namely Hilbert-Huang Transform, to a vorticity field obtained from a $8192^2$ grid-points direct numerical simulation of the 2D turbulence with a forcing scale $k_f=100$ and an Ekman friction. The measured joint probability density function $p(C,k)$ of mode $C_i(x)$ of the vorticity $\\omega$ and instantaneous wavenumber $k(x)$ is separated by the forcing scale $k_f$ into two parts, which corresponding to the inverse energy cascade and the forward enstrophy cascade. It is found that all conditional pdf $p(C\\vert k)$ at given wavenumber $k$ has an exponential tail. In the inverse energy cascade, the shape of $p(C\\vert k)$ does collapse with each other, indicating a nonintermittent cascade. The measured scaling exponent $\\zeta_{\\omega}^I(q)$ is linear with the statistical ord...

  15. Highly Sensitive Method for Titration of Adenovirus Vectors




    Authors: Hildegund Ertl, ZhiQuan Xiang, Yan Li, Dongming Zhou, Xiangyang Zhou, Wynetta Giles-Davis & Yi-lin E. Liu ### Abstract Clinical development of vaccines based on adenovirus (Ad) vectors requires accurate techniques to determine vector doses including contents of infectious particles. For vectors derived from Ad virus of human serotype 5 content of infectious particles can readily be determined by plaque assays. Vaccine vectors based on alternative Ad serotypes such as thos...

  16. New Constraints on Light Vectors Coupled to Anomalous Currents (United States)

    Dror, Jeff A.; Lasenby, Robert; Pospelov, Maxim


    We derive new constraints on light vectors coupled to standard model (SM) fermions, when the corresponding SM current is broken by the chiral anomaly. The cancellation of the anomaly by heavy fermions results, in the low-energy theory, in Wess-Zumino-type interactions between the new vector and the SM gauge bosons. These interactions are determined by the requirement that the heavy sector preserves the SM gauge groups and lead to (energy /vector mass )2 enhanced rates for processes involving the longitudinal mode of the new vector. Taking the example of a vector coupled to a vector coupled to SM baryon number, Z decays and flavor-changing neutral current meson decays via the new vector can occur with (weak scale /vector mass )2 enhanced rates. These processes place significantly stronger coupling bounds than others considered in the literature, over a wide range of vector masses.

  17. A Vortical Dawn Flank Boundary Layer for Near-Radial IMF: Wind Observations on 24 October 2001 (United States)

    Farrugia, C. J.; Gratton, F. T.; Gnavi, G.; Torbert, R. B.; Wilson, Lynn B., III


    We present an example of a boundary layer tailward of the dawn terminator which is entirely populated by rolled-up flow vortices. Observations were made by Wind on 24 October 2001 as the spacecraft moved across the region at the X plane approximately equal to -13 Earth radii. Interplanetary conditions were steady with a near-radial interplanetary magnetic field (IMF). Approximately 15 vortices were observed over the 1.5 hours duration of Wind's crossing, each lasting approximately 5 min. The rolling up is inferred from the presence of a hot tenuous plasma being accelerated to speeds higher than in the adjoining magnetosheath, a circumstance which has been shown to be a reliable signature of this in single-spacecraft observations. A blob of cold dense plasma was entrained in each vortex, at whose leading edge abrupt polarity changes of field and velocity components at current sheets were regularly observed. In the frame of the average boundary layer velocity, the dense blobs were moving predominantly sunward and their scale size along the X plane was approximately 7.4 Earth radii. Inquiring into the generation mechanism of the vortices, we analyze the stability of the boundary layer to sheared flows using compressible magnetohydrodynamic Kelvin-Helmholtz theory with continuous profiles for the physical quantities. We input parameters from (i) the exact theory of magnetosheath flow under aligned solar wind field and flow vectors near the terminator and (ii) the Wind data. It is shown that the configuration is indeed Kelvin-Helmholtz (KH) unstable. This is the first reported example of KH-unstable waves at the magnetopause under a radial IMF.


    Energy Technology Data Exchange (ETDEWEB)

    STEINWART, INGO [Los Alamos National Laboratory; HUSH, DON [Los Alamos National Laboratory; SCOVEL, CLINT [Los Alamos National Laboratory; LIST, NICOLAS [Los Alamos National Laboratory


    We show that the stopping criteria used in many support vector machine (SVM) algorithms working on the dual can be interpreted as primal optimality bounds which in turn are known to be important for the statistical analysis of SVMs. To this end we revisit the duality theory underlying the derivation of the dual and show that in many interesting cases primal optimality bounds are the same as known dual optimality bounds.

  19. Dynamics of vortices in complex wakes: Modeling, analysis, and experiments (United States)

    Basu, Saikat

    The thesis develops singly-periodic mathematical models for complex laminar wakes which are formed behind vortex-shedding bluff bodies. These wake structures exhibit a variety of patterns as the bodies oscillate or are in close proximity of one another. The most well-known formation comprises two counter-rotating vortices in each shedding cycle and is popularly known as the von Karman vortex street. Of the more complex configurations, as a specific example, this thesis investigates one of the most commonly occurring wake arrangements, which consists of two pairs of vortices in each shedding period. The paired vortices are, in general, counter-rotating and belong to a more general definition of the 2P mode, which involves periodic release of four vortices into the flow. The 2P arrangement can, primarily, be sub-classed into two types: one with a symmetric orientation of the two vortex pairs about the streamwise direction in a periodic domain and the other in which the two vortex pairs per period are placed in a staggered geometry about the wake centerline. The thesis explores the governing dynamics of such wakes and characterizes the corresponding relative vortex motion. In general, for both the symmetric as well as the staggered four vortex periodic arrangements, the thesis develops two-dimensional potential flow models (consisting of an integrable Hamiltonian system of point vortices) that consider spatially periodic arrays of four vortices with their strengths being +/-Gamma1 and +/-Gamma2. Vortex formations observed in the experiments inspire the assumed spatial symmetry. The models demonstrate a number of dynamic modes that are classified using a bifurcation analysis of the phase space topology, consisting of level curves of the Hamiltonian. Despite the vortex strengths in each pair being unequal in magnitude, some initial conditions lead to relative equilibrium when the vortex configuration moves with invariant size and shape. The scaled comparisons of the

  20. Existence and Stability of Solutions for Implicit Multivalued Vector Equilibrium Problems

    Directory of Open Access Journals (Sweden)

    Li Qiuying


    Full Text Available A class of implicit multivalued vector equilibrium problems is studied. By using the generalized Fan-Browder fixed point theorem, some existence results of solutions for the implicit multivalued vector equilibrium problems are obtained under some suitable assumptions. Moreover, a stability result of solutions for the implicit multivalued vector equilibrium problems is derived. These results extend and unify some recent results for implicit vector equilibrium problems, multivalued vector variational inequality problems, and vector variational inequality problems.

  1. The formation of sporadic E layers by a vortical perturbation excited in a horizontal wind shear flow

    Directory of Open Access Journals (Sweden)

    G. G. Didebulidze


    Full Text Available The formation of the mid-latitude sporadic E layers (Es layers by an atmospheric vortical perturbation excited in a horizontal shear flow (horizontal wind with a horizontal linear shear is investigated. A three-dimensional atmospheric vortical perturbation (atmospheric shear waves, whose velocity vector is in the horizontal plane and has a vertical wavenumber kz≠0, can provide a vertical shear of the horizontal wind. The shear waves influence the vertical transport of heavy metallic ions and their convergence into thin and dense horizontal layers. The proposed mechanism takes into account the dynamical influence of the shear wave velocity in the horizontal wind on the vertical drift velocity of the ions. It also can explain the multi-layer structure of Es layers. The pattern of the multi-layer structure depends on the value of the shear-wave vertical wavelength, the ion-neutral collision frequency and the direction of the background horizontal wind. The modelling of formation of sporadic E layers with a single and a double peak is presented. Also, the importance of shear wave coupling with short-period atmospheric gravity waves (AGWs on the variations of sporadic E layer ion density is examined and discussed.

  2. VectorBase (United States)

    U.S. Department of Health & Human Services — VectorBase is a Bioinformatics Resource Center for invertebrate vectors. It is one of four Bioinformatics Resource Centers funded by NIAID to provide web-based...

  3. Higgs Phase in a Gauge U(1 Non-Linear CP1-Model. Two Species of BPS Vortices and Their Zero Modes

    Directory of Open Access Journals (Sweden)

    Alberto Alonso-Izquierdo


    Full Text Available In this paper, zero modes of fluctuation are dissected around the two species of BPS vortices existing in the critical Higgs phase, where the scalar and vector meson masses are equal, of a gauged U ( 1 nonlinear CP 1 -model. If 2 π n , n ∈ Z , is the quantized magnetic flux of the two species of BPS vortex solutions, 2 n linearly-independent vortex zero modes for each species are found and described. The existence of two species of moduli spaces of dimension 2 n of these stringy topological defects is thus locally shown.

  4. Custodial vector model

    DEFF Research Database (Denmark)

    Becciolini, Diego; Franzosi, Diogo Buarque; Foadi, Roshan


    We analyze the Large Hadron Collider (LHC) phenomenology of heavy vector resonances with a $SU(2)_L\\times SU(2)_R$ spectral global symmetry. This symmetry partially protects the electroweak S-parameter from large contributions of the vector resonances. The resulting custodial vector model spectrum...

  5. Insulated Foamy Viral Vectors (United States)

    Browning, Diana L.; Collins, Casey P.; Hocum, Jonah D.; Leap, David J.; Rae, Dustin T.; Trobridge, Grant D.


    Retroviral vector-mediated gene therapy is promising, but genotoxicity has limited its use in the clinic. Genotoxicity is highly dependent on the retroviral vector used, and foamy viral (FV) vectors appear relatively safe. However, internal promoters may still potentially activate nearby genes. We developed insulated FV vectors, using four previously described insulators: a version of the well-studied chicken hypersensitivity site 4 insulator (650cHS4), two synthetic CCCTC-binding factor (CTCF)-based insulators, and an insulator based on the CCAAT box-binding transcription factor/nuclear factor I (7xCTF/NF1). We directly compared these insulators for enhancer-blocking activity, effect on FV vector titer, and fidelity of transfer to both proviral long terminal repeats. The synthetic CTCF-based insulators had the strongest insulating activity, but reduced titers significantly. The 7xCTF/NF1 insulator did not reduce titers but had weak insulating activity. The 650cHS4-insulated FV vector was identified as the overall most promising vector. Uninsulated and 650cHS4-insulated FV vectors were both significantly less genotoxic than gammaretroviral vectors. Integration sites were evaluated in cord blood CD34+ cells and the 650cHS4-insulated FV vector had fewer hotspots compared with an uninsulated FV vector. These data suggest that insulated FV vectors are promising for hematopoietic stem cell gene therapy. PMID:26715244

  6. Effect of genome size on AAV vector packaging. (United States)

    Wu, Zhijian; Yang, Hongyan; Colosi, Peter


    Adeno-associated virus (AAV) vector genomes have been limited to 5 kilobases (kb) in length because their packaging limit was thought to be similar to the size of the parent AAV genome. Recent reports claim that significantly larger vector genomes can be packaged intact. We examined the packaged vector genomes from plasmid-encoded AAV vectors that ranged from 4.7 to 8.7 kb in length, using AAV types 2, 5, and 8 capsids. Southern blot analysis indicated that packaged AAV vector genomes never exceeded 5.2 kb in length irrespective of the size of the plasmid-encoded vector or the capsid type. This result was confirmed by vector genome probing with strand-specific oligonucleotides. The packaged vector genomes derived from plasmid-encoded vectors exceeding 5 kb were heterogeneous in length and truncated on the 5' end. Despite their truncated genomes, vector preparations produced from plasmid-encoded vectors exceeding 5.2 kb mediated reporter gene expression in vitro at high multiplicity of infection (MOI). The efficiency of expression was substantially lower than that of reporter vectors with genomes <5 kb in length. We propose that transcriptionally functional, intact vector genomes are generated in cells transduced at high MOI from the fragmentary genomes of these larger vectors, probably by recombination.

  7. Local and Nonlocal Strain Rate Fields and Vorticity Alignment in Turbulent Flows


    Hamlington, Peter E.; Schumacher, Jörg; Dahm, Werner J. A.


    Local and nonlocal contributions to the total strain rate tensor at any point in a flow are formulated from an expansion of the vorticity field in a local spherical neighborhood of radius R centered on x. The resulting exact expression allows the nonlocal (background) strain rate tensor to be obtained from the total strain rate tensor. In turbulent flows, where the vorticity naturally concentrates into relatively compact structures, this allows the local alignment of vorticity with the most e...

  8. Velocity-vorticity formulation of three-dimensional, steady, viscous, incompressible flows

    Energy Technology Data Exchange (ETDEWEB)

    Meir, A.J. [Auburn Univ., AL (United States)


    In this work we discuss some aspects of the velocity-vorticity formulation of three-dimensional, steady, viscous, incompressible flows. We describe reasonable boundary conditions that should be imposed on the vorticity and a compatibility condition that the vorticity must satisfy. This formulation may give rise to efficient numerical algorithms for approximating solutions of the Stokes problem, which in turn yields an iterative method for approximating solutions of the Navier-Stokes equations.

  9. Time-resolved photoemission electron microscopy imaging of mode coupling between three interacting magnetic vortices

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Xiao; Cheng, X. M., E-mail: [Department of Physics, Bryn Mawr College, Bryn Mawr, Pennsylvania 19010 (United States); Keavney, D. J. [Advanced Photon Source, Argonne National Laboratory, Argonne, Illinois 60439 (United States); Asmat-Uceda, M.; Buchanan, K. S. [Department of Physics, Colorado State University, Fort Collins, Colorado 80523 (United States); Melikyan, A. [American Physical Society, Ridge, New York 11961 (United States)


    The interactions between three magnetic vortices in a planar equilateral triangular arrangement were studied by time-resolved photoemission electron microscopy. The gyrotropic resonance frequencies of the three individual vortices in the tri-disk system are different from one another and also shifted from that of an isolated vortex by as much as 12%. A comparison with analytical calculations and numerical simulations shows that the observed frequency shifts result from the dipolar interaction between the vortices.

  10. Quantized vortices in arbitrary dimensions and the normal-to-superfluid phase transition (United States)

    Bora, Florin

    The structure and energetics of superflow around quantized vortices, and the motion inherited by these vortices from this superflow, are explored in the general setting of a superfluid in arbitrary dimensions. The vortices may be idealized as objects of co-dimension two, such as one-dimensional loops and two-dimensional closed surfaces, respectively, in the cases of three- and four-dimensional superfluidity. By using the analogy between vortical superflow and Ampere-Maxwell magnetostatics, the equilibrium superflow containing any specified collection of vortices is constructed. The energy of the superflow is found to take on a simple form for vortices that are smooth and asymptotically large, compared with the vortex core size. The motion of vortices is analyzed in general, as well as for the special cases of hyper-spherical and weakly distorted hyper-planar vortices. In all dimensions, vortex motion reflects vortex geometry. In dimension four and higher, this includes not only extrinsic but also intrinsic aspects of the vortex shape, which enter via the first and second fundamental forms of classical geometry. For hyper-spherical vortices, which generalize the vortex rings of three dimensional superfluidity, the energy-momentum relation is determined. Simple scaling arguments recover the essential features of these results, up to numerical and logarithmic factors. Extending these results to systems containing multiple vortices is elementary due to the linearity of the theory. The energy for multiple vortices is thus a sum of self-energies and power-law interaction terms. The statistical mechanics of a system containing vortices is addressed via the grand canonical partition function. A renormalization-group analysis in which the low energy excitations are integrated approximately, is used to compute certain critical coefficients. The exponents obtained via this approximate procedure are compared with values obtained previously by other means. For dimensions higher

  11. Vortex identification from local properties of the vorticity field (United States)

    Elsas, J. H.; Moriconi, L.


    A number of systematic procedures for the identification of vortices/coherent structures have been developed as a way to address their possible kinematical and dynamical roles in structural formulations of turbulence. It has been broadly acknowledged, however, that vortex detection algorithms, usually based on linear-algebraic properties of the velocity gradient tensor, can be plagued with severe shortcomings and may become, in practical terms, dependent on the choice of subjective threshold parameters in their implementations. In two-dimensions, a large class of standard vortex identification prescriptions turn out to be equivalent to the "swirling strength criterion" (λc i-criterion), which is critically revisited in this work. We classify the instances where the accuracy of the λc i-criterion is affected by nonlinear superposition effects and propose an alternative vortex detection scheme based on the local curvature properties of the vorticity graph (x ,y ,ω ) —the "vorticity curvature criterion" (λω-criterion)—which improves over the results obtained with the λc i-criterion in controlled Monte Carlo tests. A particularly problematic issue, given its importance in wall-bounded flows, is the eventual inadequacy of the λc i-criterion for many-vortex configurations in the presence of strong background shear. We show that the λω-criterion is able to cope with these cases as well, if a subtraction of the mean velocity field background is performed, in the spirit of the Reynolds decomposition procedure. A realistic comparative study for vortex identification is then carried out for a direct numerical simulation of a turbulent channel flow, including a three-dimensional extension of the λω-criterion. In contrast to the λc i-criterion, the λω-criterion indicates in a consistent way the existence of small scale isotropic turbulent fluctuations in the logarithmic layer, in consonance with long-standing assumptions commonly taken in turbulent boundary

  12. Vector description of nonlinear magnetization

    Energy Technology Data Exchange (ETDEWEB)

    Rysak, A. E-mail:; Korczak, S.Z


    The definition of the nonlinear magnetization, which is usually applied in investigating superconductors is discussed in this paper. The nonlinear magnetization of the superconductor, as the response to the alternating magnetic field, is a periodic time function and is determined by the sequence of complex numbers {chi}{sub n}={chi}{sub n}'+i{chi}{sub n}'', n=1,2,3,.... The sequence {chi}{sub n} is named harmonic susceptibility. Finding some limits in this definition we propose a new description, substituting the complex sequence {chi}{sub n} with the sequence of real covariant vectors. Such a definition of the nonlinear magnetization, allows to determine function M(t) of an investigated system for an arbitrary initial phase {theta}{sub o} of the inducing magnetic field H{sub ac}=h{sub ac} sin({omega}t+{theta}{sub o}). Vector description, in our opinion, is superior to the complex one in respect of explicitness, simplicity, and universality. Circular diagrams are used to illustrate the new vector description. We also show how the vectors of the harmonic susceptibility can be derived from both numeric calculations and experimental data.

  13. Macro motion vector quantization (United States)

    Lee, Yoon Y.; Woods, John W.


    A new algorithm is developed for reducing the bit rate required for motion vectors. This algorithm is a generalization of block matching motion estimation in which the search region is represented as a codebook of motion vectors. The new algorithm, called macro motion vector quantization (MMVQ), generalized our earlier MVQ by coding a group of motion vectors. The codebook is a set of macro motion vectors which represent the block locations of the small neighboring blocks in the previous frame. We develop an interative design algorithm for the codebook. Our experiments show that the variances of displaced frame differences (DFDs) are reduced significantly compared to block matching algorithm (BMA) with the macroblock size.

  14. Nonlinear modes in the hollow-cores of liquid vortices

    KAUST Repository

    Amaouche, Mustapha


    In this paper we show that the wave patterns observed on the interfacial contours of hollow-core vortices, produced within a shallow layer of fluid contained in stationary cylinder and driven by a rotating disk at the bottom [G.H. Vatistas, H.A. Abderrahmane, M.H. Kamran Siddiqui, Experimental confirmation of Kelvin\\'s equilibria, Phys. Rev. Lett. 100 (2008) 174503-174504], can be described as travelling cnoidal waves. These rotating stationary waves are obtained as solutions of a Korteweg-de Vries type equation, in accordance with the geometrical and kinematic characteristics of the observed polygonal patterns. © 2013 Elsevier Masson SAS. All rights reserved.

  15. How hairpin vortices emerge from exact invariant solutions (United States)

    Schneider, Tobias M.; Farano, Mirko; de Palma, Pietro; Robinet, Jean-Christoph; Cherubini, Stefania


    Hairpin vortices are among the most commonly observed flow structures in wall-bounded shear flows. However, within the dynamical system approach to turbulence, those structures have not yet been described. They are not captured by known exact invariant solutions of the Navier-Stokes equations nor have other state-space structures supporting hairpins been identified. We show that hairpin structures are observed along an optimally growing trajectory leaving a well known exact traveling wave solution of plane Poiseuille flow. The perturbation triggering hairpins does not correspond to an unstable mode of the exact traveling wave but lies in the stable manifold where non-normality causes strong transient amplification.

  16. Spins in the vortices of a high-temperature superconductor

    DEFF Research Database (Denmark)

    Lake, B.; Aeppli, G.; Clausen, K.N.


    Neutron scattering is used to characterize the magnetism of the vortices for the optimally doped high-temperature superconductor La2-xSrxCuO4 (x = 0.163) in an applied magnetic field. As temperature is reduced, Low-frequency spin fluctuations first disappear with the loss of vortex mobility......, but then reappear. We find that the vortex state can be regarded as an inhomogeneous mixture of a superconducting spin fluid and a material containing a nearly ordered antiferromagnet. These experiments show that as for many other properties of cuprate superconductors, the important underlying microscopic forces...

  17. A turbulence model for buoyant flows based on vorticity generation.

    Energy Technology Data Exchange (ETDEWEB)

    Domino, Stefan Paul; Nicolette, Vernon F.; O' Hern, Timothy John; Tieszen, Sheldon R.; Black, Amalia Rebecca


    A turbulence model for buoyant flows has been developed in the context of a k-{var_epsilon} turbulence modeling approach. A production term is added to the turbulent kinetic energy equation based on dimensional reasoning using an appropriate time scale for buoyancy-induced turbulence taken from the vorticity conservation equation. The resulting turbulence model is calibrated against far field helium-air spread rate data, and validated with near source, strongly buoyant helium plume data sets. This model is more numerically stable and gives better predictions over a much broader range of mesh densities than the standard k-{var_epsilon} model for these strongly buoyant flows.

  18. The numerical solution of the vorticity transport equation

    CERN Document Server

    Dennis, S C R


    A method of approximating the two-dimensional vorticity transport equation in which the matrix associated with the difference equations is diagonally dominant and the truncation error is the same as that of the fully central-difference approximation, is discussed. An example from boundary layer theory is given by calculating the viscous stagnation point flow at the nose of a cylinder. Some new solutions of the Navier-Stokes equations are obtained for symmetrical flow past a flat plate of finite length. (16 refs).

  19. The influence of trailed vorticity on flutter speed estimations

    DEFF Research Database (Denmark)

    Pirrung, Georg; Aagaard Madsen, Helge; Kim, Taeseong


    ) theory, which is coupled with Beddoes' near wake model for trailed vorticity. The first part of this work outlines the implementation in HAWC2, with a focus on the interaction of the induction from the blade based near wake model with the induction from the polar grid based BEM model in HAWC2...... building up at a critical rotor speed. Blades with modified torsional and flapwise stiffness are also investigated. A flutter analysis is often part of the stability investigations for new blades but is normally carried out with engineering models that do not include the influence of unsteady trailed...

  20. Topological charge algebra of optical vortices in nonlinear interactions. (United States)

    Zhdanova, Alexandra A; Shutova, Mariia; Bahari, Aysan; Zhi, Miaochan; Sokolov, Alexei V


    We investigate the transfer of orbital angular momentum among multiple beams involved in a coherent Raman interaction. We use a liquid crystal light modulator to shape pump and Stokes beams into optical vortices with various integer values of topological charge, and cross them in a Raman-active crystal to produce multiple Stokes and anti-Stokes sidebands. We measure the resultant vortex charges using a tilted-lens technique. We verify that in every case the generated beams' topological charges obey a simple relationship, resulting from angular momentum conservation for created and annihilated photons, or equivalently, from phase-matching considerations for multiple interacting beams.

  1. Close pairs of relative equilibria for identical point vortices

    DEFF Research Database (Denmark)

    Dirksen, Tobias; Aref, Hassan


    Numerical solution of the classical problem of relative equilibria for identical point vortices on the unbounded plane reveals configurations that are very close to the analytically known, centered, symmetrically arranged, nested equilateral triangles. New numerical solutions of this kind are found...... also has this property, and new relative equilibria close to the nested, symmetrically arranged, regular heptagons have been found. The centered regular nonagon is also marginally stable. Again, a new family of close relative equilibria has been found. The closest relative equilibrium pairs occur...

  2. Non-Abelian statistics of vortices with non-Abelian Dirac fermions. (United States)

    Yasui, Shigehiro; Hirono, Yuji; Itakura, Kazunori; Nitta, Muneto


    We extend our previous analysis on the exchange statistics of vortices having a single Dirac fermion trapped in each core to the case where vortices trap two Dirac fermions with U(2) symmetry. Such a system of vortices with non-Abelian Dirac fermions appears in color superconductors at extremely high densities and in supersymmetric QCD. We show that the exchange of two vortices having doublet Dirac fermions in each core is expressed by non-Abelian representations of a braid group, which is explicitly verified in the matrix representation of the exchange operators when the number of vortices is up to four. We find that the result contains the matrices previously obtained for the vortices with a single Dirac fermion in each core as a special case. The whole braid group does not immediately imply non-Abelian statistics of identical particles because it also contains exchanges between vortices with different numbers of Dirac fermions. However, we find that it does contain, as its subgroup, genuine non-Abelian statistics for the exchange of the identical particles, that is, vortices with the same number of Dirac fermions. This result is surprising compared with conventional understanding because all Dirac fermions are defined locally at each vortex, unlike the case of Majorana fermions for which Dirac fermions are defined nonlocally by Majorana fermions located at two spatially separated vortices.

  3. Acoustic Virtual Vortices with Tunable Orbital Angular Momentum for Trapping of Mie Particles (United States)

    Marzo, Asier; Caleap, Mihai; Drinkwater, Bruce W.


    Acoustic vortices can transfer angular momentum and trap particles. Here, we show that particles trapped in airborne acoustic vortices orbit at high speeds, leading to dynamic instability and ejection. We demonstrate stable trapping inside acoustic vortices by generating sequences of short-pulsed vortices of equal helicity but opposite chirality. This produces a "virtual vortex" with an orbital angular momentum that can be tuned independently of the trapping force. We use this method to adjust the rotational speed of particles inside a vortex beam and, for the first time, create three-dimensional acoustics traps for particles of wavelength order (i.e., Mie particles).

  4. Prescribed Velocity Gradients for Highly Viscous SPH Fluids with Vorticity Diffusion. (United States)

    Peer, Andreas; Teschner, Matthias


    Working with prescribed velocity gradients is a promising approach to efficiently and robustly simulate highly viscous SPH fluids. Such approaches allow to explicitly and independently process shear rate, spin, and expansion rate. This can be used to, e.g., avoid interferences between pressure and viscosity solvers. Another interesting aspect is the possibility to explicitly process the vorticity, e.g., to preserve the vorticity. In this context, this paper proposes a novel variant of the prescribed-gradient idea that handles vorticity in a physically motivated way. In contrast to a less appropriate vorticity preservation that has been used in a previous approach, vorticity is diffused. The paper illustrates the utility of the vorticity diffusion. Therefore, comparisons of the proposed vorticity diffusion with vorticity preservation and additionally with vorticity damping are presented. The paper further discusses the relation between prescribed velocity gradients and prescribed velocity Laplacians which improves the intuition behind the prescribed-gradient method for highly viscous SPH fluids. Finally, the paper discusses the relation of the proposed method to a physically correct implicit viscosity formulation.

  5. Quantized vortices and superflow in arbitrary dimensions: structure, energetics and dynamics (United States)

    Goldbart, Paul M.; Bora, Florin


    The structure and energetics of superflow around quantized vortices, and the motion inherited by these vortices from this superflow, are explored in the general setting of a superfluid in arbitrary dimensions. The vortices may be idealized as objects of codimension 2, such as one-dimensional loops and two-dimensional closed surfaces, respectively, in the cases of three- and four-dimensional superfluidity. By using the analogy between the vortical superflow and Ampère-Maxwell magnetostatics, the equilibrium superflow containing any specified collection of vortices is constructed. The energy of the superflow is found to take on a simple form for vortices that are smooth and asymptotically large, compared with the vortex core size. The motion of vortices is analyzed in general, as well as for the special cases of hyper-spherical and weakly distorted hyper-planar vortices. In all dimensions, vortex motion reflects vortex geometry. In dimension 4 and higher, this includes not only extrinsic but also intrinsic aspects of the vortex shape, which enter via the first and second fundamental forms of classical geometry. For hyper-spherical vortices, which generalize the vortex rings of three-dimensional superfluidity, the energy-momentum relation is determined. Simple scaling arguments recover the essential features of these results, up to numerical and logarithmic factors.

  6. Toroidal Geometry Stabilizing a Latitudinal Ring of Point Vortices on a Torus (United States)

    Sakajo, Takashi; Shimizu, Yuuki


    We carry out the linear stability analysis of a polygonal ring configuration of N point vortices, called an N-ring, along the line of latitude θ _0 on a torus with the aspect ratio α . Deriving a criterion for the stability depending on the parameters N, θ _0 and α , we reveal how the aspect ratio α contributes to the stability of the N-ring. While the N-ring necessarily becomes unstable when N is sufficiently large for fixed α , the stability is closely associated with the geometric property of the torus for variable α ; for low aspect ratio α ˜ 1 , N=7 is a critical number determining the stability of the N-ring when it is located along a certain range of latitudes, which is an analogous result to those in a plane and on a sphere. On the other hand, the stability is determined by the sign of curvature for high aspect ratio α ≫ 1 . That is to say, the N-ring is neutrally stable if it is located on the inner side of the toroidal surface with a negative curvature, while the N-ring on its outer side with a positive curvature is unstable. Furthermore, based on the linear stability analysis, we describe nonlinear evolution of the N-ring when it becomes unstable. It is difficult to deal with this problem, since the evolution equation of the N point vortices is formulated as a Hamiltonian system with N degrees of freedom, which is in general non-integrable. Thus, we reduce the Hamiltonian system to a simple integrable system by introducing a cyclic symmetry. Owing to this reduction, we successfully find some periodic orbits in the reduced system, whose local bifurcations and global transitions for variable α are characterized in terms of the fundamental group of the torus.

  7. Shallow water modeling of Jovian polar cyclone and vortices (United States)

    Li, Cheng; Tabataba-Vakili, Fachreddin; Ingersoll, Andrew P.


    Jupiter’s polar atmosphere was observed for the first time by the Juno visible spectrum camera (JunoCAM) and Juno Infrared Auroral Mapper (JIRAM). Both the visible and infrared images show active vortices and weather systems that are unlike any polar regions previously seen or modeled on any of the planets in our solar system. We developed a global shallow water model on a sphere with poles rotated to the equator to investigate the formation, maintenance and dynamic regimes controlling the morphology of polar cyclones and vortices. Passive Lagrangian particles with finite life time are included to represent the clouds. We verified that a westward barotropically unstable jet can spontaneously break the axial symmetry into a polygon-shaped figure rotating rigidly around the rotation axis as reported by previous laboratory experiments. The number of sides of the polygon depends on the deformation radius and is insensitive to the initial condition. Why Jupiter’s pole is different from Saturn’s is still under investigation.

  8. Classification of hemispheric monthly mean stratospheric potential vorticity fields

    Directory of Open Access Journals (Sweden)

    R. Huth


    Full Text Available Monthly mean NCEP reanalysis potential vorticity fields at the 650 K isentropic level over the Northern and Southern Hemispheres between 1979 and 1997 were studied using multivariate analysis tools. Principal component analysis in the T-mode was applied to demonstrate the validity of such statistical techniques for the study of stratospheric dynamics and climatology. The method, complementarily applied to both the raw and anomaly fields, was useful in determining and classifying the characteristics of winter and summer PV fields on both hemispheres, in particular, the well-known differences in the behaviour and persistence of the polar vortices. It was possible to identify such features as sudden warming events in the Northern Hemisphere and final warming dates in both hemispheres. The stratospheric impact of other atmospheric processes, such as volcanic eruptions, also identified though the results, must be viewed at this stage as tentative. An interesting change in behaviour around 1990 was detected over both hemispheres.Key words. Meteorology and atmospheric dynamics (middle atmosphere dynamics; general circulation; climatology

  9. Classification of hemispheric monthly mean stratospheric potential vorticity fields

    Directory of Open Access Journals (Sweden)

    R. Huth

    Full Text Available Monthly mean NCEP reanalysis potential vorticity fields at the 650 K isentropic level over the Northern and Southern Hemispheres between 1979 and 1997 were studied using multivariate analysis tools. Principal component analysis in the T-mode was applied to demonstrate the validity of such statistical techniques for the study of stratospheric dynamics and climatology. The method, complementarily applied to both the raw and anomaly fields, was useful in determining and classifying the characteristics of winter and summer PV fields on both hemispheres, in particular, the well-known differences in the behaviour and persistence of the polar vortices. It was possible to identify such features as sudden warming events in the Northern Hemisphere and final warming dates in both hemispheres. The stratospheric impact of other atmospheric processes, such as volcanic eruptions, also identified though the results, must be viewed at this stage as tentative. An interesting change in behaviour around 1990 was detected over both hemispheres.

    Key words. Meteorology and atmospheric dynamics (middle atmosphere dynamics; general circulation; climatology

  10. EFT for Vortices with Dilaton-dependent Localized Flux

    CERN Document Server

    Burgess, C P; Williams, M


    We study how codimension-two objects like vortices back-react gravitationally with their environment in theories (such as 4D or higher-dimensional supergravity) where the bulk is described by a dilaton-Maxwell-Einstein system. We do so both in the full theory, for which the vortex is an explicit classical `fat brane' solution, and in the effective theory of `point branes' appropriate when the vortices are much smaller than the scales of interest for their back-reaction (such as the transverse Kaluza-Klein scale). We extend the standard Nambu-Goto description to include the physics of flux-localization wherein the ambient flux of the external Maxwell field becomes partially localized to the vortex, generalizing the results of a companion paper to include dilaton-dependence for the tension and localized flux. In the effective theory, such flux-localization is described by the next-to-leading effective interaction, and the boundary conditions to which it gives rise are known to play an important role in how (and...

  11. Bielectron vortices in two-dimensional Dirac semimetals. (United States)

    Downing, C A; Portnoi, M E


    Searching for new states of matter and unusual quasi-particles in emerging materials and especially low-dimensional systems is one of the major trends in contemporary condensed matter physics. Dirac materials, which host quasi-particles which are described by ultrarelativistic Dirac-like equations, are of a significant current interest from both a fundamental and applied physics perspective. Here we show that a pair of two-dimensional massless Dirac-Weyl fermions can form a bound state independently of the sign of the inter-particle interaction potential, as long as this potential decays at large distances faster than Kepler's inverse distance law. This leads to the emergence of a new type of energetically favorable quasiparticle: bielectron vortices, which are double-charged and reside at zero-energy. Their bosonic nature allows for condensation and may give rise to Majorana physics without invoking a superconductor. These novel quasi-particles arguably explain a range of poorly understood experiments in gated graphene structures at low doping.Two-dimensional Dirac semimetals are known to host fermionic excitations which can mimic physics usually found in ultrarelativistic quantum mechanics. Here, the authors unveil the existence of another type of quasiparticle, bielectron vortices, which are bosonic and may give rise to new types of condensates.

  12. NonBoussinesq effects on vorticity and kinetic energy production (United States)

    Ravichandran, S.; Dixit, Harish; Govindarajan, Rama


    The Boussinesq approximation, commonly employed in weakly compressible or incompressible flows, neglects changes in inertia due to changes in the density. However, the nonBoussinesq terms can lead to a kind of centrifugal instability for small but sharp density variations, and therefore cannot be neglected under such circumstances (see, e.g., DIXIT & GOVINDARAJAN, JFM , 2010, 415). Here, we study the evolution of a light-cored Gaussian vortex and find that the nonBoussinesq terms can lead to significant changes in how vortices evolve. The problem is governed by three nondimensional numbers--Reynolds number (i.e. viscosity), Atwood number, and a ratio of gravitational and centrifugal Froude numbers. We find that the production of kinetic energy and vorticity in a light-cored Gaussian vortex are affected significantly by the nonBoussinesq terms, and varies non-monotonically with the parameters of the problem. In general, these nonBoussinesq effects depend both on the strength of gravity and on the Reynolds number associated with the initial vortex.

  13. Generalized Hasimoto Transform, Binormal Flow and Quantized Vortices (United States)

    Strong, Scott A.; Carr, Lincoln D.


    A quantized vortex is a topological object central to the study of quantum liquids. Current models of vortex dynamics are motivated by the nonlinear Schrödingier equation and porting techniques from classical vortices. Self induction of classical vorticity ideally localized to a space curve asserts that a curved vortex filament propagates at a speed proportional to its curvature, | v | ~ κ , in the binormal direction of the Frenet frame, b& circ;. Interestingly, this autonomous dynamic can be mapped into the space of solutions to a cubic focusing nonlinear Schrödinger equation, iψt +ψss +1/2| ψ | 2 ψ = 0 , where ψ is a plane-wave defined by curvature and torsion of the vortex filament, ψ = κexp [ i ∫ dsτ ] . Using these two results, one can define a vortex configuration, within superfluid helium or a Bose-Einstein condensate, and prescribe a binormal evolution. In general, however, binormal flow depends nonlinearly on local curvature and maps to a class of nonlinear integro-differential Schrödinger equations. In this talk we discuss how system size affects higher-order nonlinearity and filament geometry which is applicable to theoretical and numerical investigations of vortex dominated quantum hydrodynamics. Funded by NSF.

  14. Implicit Real Vector Automata

    Directory of Open Access Journals (Sweden)

    Jean-François Degbomont


    Full Text Available This paper addresses the symbolic representation of non-convex real polyhedra, i.e., sets of real vectors satisfying arbitrary Boolean combinations of linear constraints. We develop an original data structure for representing such sets, based on an implicit and concise encoding of a known structure, the Real Vector Automaton. The resulting formalism provides a canonical representation of polyhedra, is closed under Boolean operators, and admits an efficient decision procedure for testing the membership of a vector.

  15. Vectors and their applications

    CERN Document Server

    Pettofrezzo, Anthony J


    Geared toward undergraduate students, this text illustrates the use of vectors as a mathematical tool in plane synthetic geometry, plane and spherical trigonometry, and analytic geometry of two- and three-dimensional space. Its rigorous development includes a complete treatment of the algebra of vectors in the first two chapters.Among the text's outstanding features are numbered definitions and theorems in the development of vector algebra, which appear in italics for easy reference. Most of the theorems include proofs, and coordinate position vectors receive an in-depth treatment. Key concept

  16. Potential Vorticity Evolution in the Co-orbital Region of Embedded Protoplanets

    Energy Technology Data Exchange (ETDEWEB)

    Koller, J. [Rice Univ., Houston, TX (United States)


    This thesis presents two-dimensional hydrodynamic disk simulations with embedded protoplanets, emphasizing the non-linear dynamics in the co-orbital region. In particular, it demonstrates how a protoplanetary disk responds to embedded low mass planets at the inviscid limit. Since the potential vorticity (PV) flow is not conserved, due to the spiral shocks and possibly boundary layer effects emanating from the planet, the PV profile develops inflection points which eventually render the flow unstable. Vortices are produced in association with the potential vorticity minima. Born in the separatrix region, these vortices experience close encounters with the planet, consequently exerting strong torques on the planet. The existence of these vortices, if confirmed, have important implications on planetary migration rates. The formation of vortices is discussed in more detail and a key parameter is found which depends solely on planet mass and sound speed. With this key parameter, one can predict the disk evolution, PV growth rates, and threshold conditions for forming vortices in the co-orbital region. An analytical estimate for the change of PV due to shocks is compared to the actual change in PV in the hydrodynamic simulations. They match well except in the inner region where vortices form. In addition, extensive resolution tests were carried out but uncertainties remain about the physics of this particular region.

  17. Dynamics of fractional vortices in long Josephson junctions; Dynamik fraktionaler Flusswirbel in langen Josephsonkontakten

    Energy Technology Data Exchange (ETDEWEB)

    Gaber, Tobias


    In this thesis static and dynamic properties of fractional vortices in long Josephson junctions are investigated. Fractional vortices are circulating supercurrents similar to the well-known Josephson fluxons. Yet, they show the distinguishing property of carrying only a fraction of the magnetic flux quantum. Fractional vortices are interesting non-linear objects. They spontaneously appear and are pinned at the phase discontinuity points of so called 0-{kappa} junctions but can be bend or flipped by external forces like bias currents or magnetic fields. 0-{kappa} junctions and fractional vortices are generalizations of the well-known 0-{pi} junctions and semifluxons, where not only phase jumps of pi but arbitrary values denoted by kappa are considered. By using so-called artificial 0-{kappa} junctions that are based on standard Nb-AlO{sub x}-Nb technology the classical dynamics of fractional vortices has been investigated experimentally for the very first time. Here, half-integer zero field steps could be observed. These voltage steps on the junction's current-voltage characteristics correspond to the periodic flipping/hopping of fractional vortices. In addition, the oscillatory eigenmodes of fractional vortices were investigated. In contrast to fluxons fractional vortices have an oscillatory eigenmode with a frequency within the plasma gap. Using resonance spectroscopy the dependence of the eigenmode frequency on the flux carried by the vortex and an applied bias current was determined. (orig.)

  18. A numerical study of the role of the vertical structure of vorticity during tropical cyclone genesis

    Energy Technology Data Exchange (ETDEWEB)

    Venkatesh, T N [Flosolver Unit, National Aerospace Laboratories, PO Box 1779, Bangalore 560017 (India); Mathew, Joseph, E-mail: tnv@flosolver.nal.res.i, E-mail: joseph@aero.iisc.ernet.i [Department of Aerospace Engineering, Indian Institute of Science, Bangalore 560012 (India)


    An eight-level axisymmetric model with simple parameterizations for clouds and the atmospheric boundary layer was developed to examine the evolution of vortices that are precursors to tropical cyclones. The effect of vertical distributions of vorticity, especially that arising from a merger of mid-level vortices, was studied by us to provide support for a new vortex-merger theory of tropical cyclone genesis. The basic model was validated with the analytical results available for the spin-down of axisymmetric vortices. With the inclusion of the cloud and boundary layer parameterizations, the evolution of deep vortices into hurricanes and the subsequent decay are simulated quite well. The effects of several parameters such as the initial vortex strength, radius of maximum winds, sea-surface temperature and latitude (Coriolis parameter) on the evolution were examined. A new finding is the manner in which mid-level vortices of the same strength decay and how, on simulated merger of these mid-level vortices, the resulting vortex amplifies to hurricane strength in a realistic time frame. The importance of sea-surface temperature on the evolution of full vortices was studied and explained. Also it was found that the strength of the surface vortex determines the time taken by the deep vortex to amplify to hurricane strength.

  19. Imparting small vorticity to a Bianchi type-VIh empty spacetime (United States)

    Batakis, Nikos A.


    We present and briefly discuss a Bianchi type-VIh empty spacetime. The field equations have been solved after being linearized with respect to a parameter which imparts vorticity to the model. The limit of zero vorticity is an already known solution.

  20. Dynamic manipulation of asymmetric forebody vortices to achieve linear control (United States)

    Lee, Richard

    A wind tunnel experiment was performed to further investigate the potential of the dynamic manipulation of forebody vortices as a means of supplementing directional control of fighter aircraft at high angles of attack. Tests were conducted on a 65-deg delta-wing model fitted with a slender, pointed tangent-ogive forebody of circular cross-section and 12.8 deg semi-apex angle. Forward-blowing nozzles located near the apex of the forebody served as the means of manipulating the forebody vortices. As expected, forward blowing was very effective, i.e., little blowing effort was required to cause the forebody vortex on the blown side to assume the 'high' position. However, the magnitudes of yawing moment and side force developed by the slender forebody with blowing do not differ significantly from that of the no-blowing, baseline case. Moreover, blowing above a certain threshold value produced an unexpected reversal, with blowing causing the vortex on the blown side to assume the 'low' position instead and the yawing moment and side force to change sense. The results have shown that the dynamic manipulation scheme is very successful in producing a linear variation of time-average yawing moment with a duty-cycle parameter, even with sideslip, for the aircraft-like model. The results also show that, by switching the vortex pattern rapidly, the linearity can be maintained up to a reduced frequency of at least 0.32, which is expected to be very satisfactory for practical applications. A subsequent water tunnel experiment with the forebody alone was undertaken to conduct off-surface flow visualizations that confirmed the vortex reversal phenomenon. Based on the flow visualization studies, a hypothesis was formed regarding the cause of the reversal phenomenon; it postulates that at the reversal threshold the nozzle flux interrupts the formation of the high forebody vortex on the blowing side and encourages the shear layer to form a replacement vortex that lies close to the

  1. Matter in the form of toroidal electromagnetic vortices (United States)

    Hagen, Wilhelm F.


    The creation of charged elementary particles from neutral photons is explained as a conversion process of electromagnetic (EM) energy from linear to circular motion at the speed of light into two localized, toroidal shaped vortices of trapped EM energy that resist change of motion, perceptible as particles with inertia and hence mass. The photon can be represented as a superposition of left and right circular polarized transverse electric fields of opposite polarity originating from a common zero potential axis, the optical axis of the photon. If these components are separated by interaction with a strong field (nucleon) they would curl up into two electromagnetic vortices (EMV) due to longitudinal magnetic field components forming toroids. These vortices are perceptible as opposite charged elementary particles e+/- . These spinning toroids generate extended oscillating fields that interact with stationary field oscillations. The velocity-dependent frequency differences cause beat signals equivalent to matter waves, leading to interference. The extended fields entangled with every particle explain wave particle duality issues. Spin and magnetic moment are the natural outcome of these gyrating particles. As the energy and hence mass of the electron increases with acceleration so does its size shrink proportional to its reduced wavelength. The artificial weak and strong nuclear forces can be easily explained as different manifestations of the intermediate EM forces. The unstable neutron consists of a proton surrounded by a contracted and captured electron. The associated radial EM forces represent the weak nuclear force. The deuteron consists of two axially separated protons held together by a centrally captured electron. The axial EM forces represent the strong nuclear force, providing stability for "neutrons" only within nucleons. The same principles were applied to determine the geometries of force-balanced nuclei. The alpha-particle emerges as a very compact

  2. Vortical Motions of Baryonic Gas in the Cosmic Web: Growth History and Scaling Relation (United States)

    Zhu, Weishan; Feng, Long-long


    The vortical motions of the baryonic gas residing in large-scale structures are investigated by cosmological hydrodynamic simulations. Proceeding in the formation of the cosmic web, the vortical motions of baryonic matter are pumped up by baroclinity in two stages, i.e., the formation of sheets and filaments. The mean curl velocities are about filaments, and knots at z = 0, respectively. The scaling of the vortical velocity of gas can be well described by the She-Leveque hierarchical turbulence model in the range of l filaments, Df ˜ 1.9-2.2, and smaller than the fractal dimension of sheets, Ds ˜ 2.4-2.7. The vortical kinetic energy of baryonic gas is mainly transported by filaments. Both scalings of mass distribution and vortical velocity increments show distinctive transitions at the turning scale of ˜0.65(1.50) h-1 Mpc, which may be closely related to the characteristic radius of density filaments.

  3. Numerical and Experimental Study on Negative Buoyance Induced Vortices in N-Butane Jet Flames

    KAUST Repository

    Xiong, Yuan


    Near nozzle flow field in flickering n-butane diffusion jet flames was investigated with a special focus on transient flow patterns of negative buoyance induced vortices. The flow structures were obtained through Mie scattering imaging with seed particles in a fuel stream using continuous-wave (CW) Argon-ion laser. Velocity fields were also quantified with particle mage velocimetry (PIV) system having kHz repetition rate. The results showed that the dynamic motion of negative buoyance induced vortices near the nozzle exit was coupled strongly with a flame flickering instability. Typically during the flame flickering, the negative buoyant vortices oscillated at the flickering frequency. The vortices were distorted by the flickering motion and exhibited complicated transient vortical patterns, such as tilting and stretching. Numerical simulations were also implemented based on an open source C++ package, LaminarSMOKE, for further validations.

  4. Indirect Combustion Noise: Noise Generation by Accelerated Vorticity in a Nozzle Flow

    Directory of Open Access Journals (Sweden)

    Nancy Kings


    Full Text Available The noise generation by accelerated vorticity waves in a nozzle flow was investigated in a model experiment. This noise generation mechanism belongs, besides entropy noise, to the indirect combustion noise phenomena. Vorticity as well as entropy fluctuations, originating from the highly turbulent combustion zone, are convected with the flow and produce noise during their acceleration in the outlet nozzle of the combustion chamber. In the model experiment, noise generation of accelerated vorticity fluctuations was achieved. The vorticity fluctuations in the tube flow were produced by injecting temporally additional air into the mean flow. As the next step, a parametric study was conducted to determine the major dependencies of the so called vortex noise. A quadratic dependency of the vortex noise on the injected air amount was found. In order to visualise and classify the artificially generated vorticity structures, planar velocity measurements have been conducted applying Particle Image Velocimetry (PIV.

  5. Viral vector-based influenza vaccines (United States)

    de Vries, Rory D.; Rimmelzwaan, Guus F.


    ABSTRACT Antigenic drift of seasonal influenza viruses and the occasional introduction of influenza viruses of novel subtypes into the human population complicate the timely production of effective vaccines that antigenically match the virus strains that cause epidemic or pandemic outbreaks. The development of game-changing vaccines that induce broadly protective immunity against a wide variety of influenza viruses is an unmet need, in which recombinant viral vectors may provide. Use of viral vectors allows the delivery of any influenza virus antigen, or derivative thereof, to the immune system, resulting in the optimal induction of virus-specific B- and T-cell responses against this antigen of choice. This systematic review discusses results obtained with vectored influenza virus vaccines and advantages and disadvantages of the currently available viral vectors. PMID:27455345

  6. VEST: Abstract vector calculus simplification in Mathematica (United States)

    Squire, J.; Burby, J.; Qin, H.


    We present a new package, VEST (Vector Einstein Summation Tools), that performs abstract vector calculus computations in Mathematica. Through the use of index notation, VEST is able to reduce three-dimensional scalar and vector expressions of a very general type to a well defined standard form. In addition, utilizing properties of the Levi-Civita symbol, the program can derive types of multi-term vector identities that are not recognized by reduction, subsequently applying these to simplify large expressions. In a companion paper Burby et al. (2013) [12], we employ VEST in the automation of the calculation of high-order Lagrangians for the single particle guiding center system in plasma physics, a computation which illustrates its ability to handle very large expressions. VEST has been designed to be simple and intuitive to use, both for basic checking of work and more involved computations.

  7. Differential effects of lentiviral vector-mediated overexpression of nerve growth factor and glial cell line-derived neurotrophic factor on regenerating sensory and motor axons in the transected peripheral nerve

    NARCIS (Netherlands)

    Tannemaat, Martijn R; Eggers, Ruben; Hendriks, William T; de Ruiter, Godard C W; van Heerikhuize, Joop J; Pool, Chris W; Malessy, Martijn J A; Boer, Gerard J; Verhaagen, J.


    Even after reconstructive surgery, major functional impairments remain in the majority of patients with peripheral nerve injuries. The application of novel emerging therapeutic strategies, such as lentiviral (LV) vectors, may help to stimulate peripheral nerve regeneration at a molecular level. In

  8. Correlation analysis on volume vorticity and vortex in late boundary layer transition (United States)

    Dong, Xiangrui; Tian, Shuling; Liu, Chaoqun


    In this paper, two functions are introduced to describe the turbulence generation in late flow transition. One is called the volume omega bar (volume Ω ¯ ), which represents the flow rotation or vortex strength. The other is called the volume vorticity, which shows the flow statistical fluctuations. Although they have very different definitions, one is for fluctuation and the other is for rotation, volume Ω ¯ and volume vorticity are found highly correlated with a correlation factor greater than 0.9, which means that there is a very close correlation between flow fluctuation and flow rotation (vortex). While the vorticity flux keeps constant in the late flow transition through the integration over any sections either parallel or perpendicular to the flow direction, the volume Ω ¯ is greatly increased along the flow direction during the flow transition process. This means that the vortex structures are greatly built up and rotation becomes more and more dominant. On the other hand, the total volume vorticity is also quickly increased, which shows that the fluctuation is stronger. The flow transition is a process with significant volume vorticity increase, which is attributed to two of the following crucial factors: the first one is the lengthening of the vorticity tubes from side boundary due to the vorticity line stretching, distortion, and tangling; the second one is the generation of countless self-closed vorticity rings within the domain. Both the increase of the volume vorticity and the volume Ω ¯ can be a significant symbol of the flow transition from the laminar flow to turbulent flow. It also shows that vorticity (tubes or lines) cannot directly represent vortex and should not be simply used as a signal of the turbulent transition process.

  9. Population, characteristics and kinematics of vortices in a confined rectangular jet with a co-flow (United States)

    Kong, B.; Olsen, M. G.; Fox, R. O.; Hill, J. C.


    Vortex behavior and characteristics in a confined rectangular jet with a co-flow were examined using vortex swirling strength as a defining characteristic. On the left side of the jet, the positively (counterclockwise) rotating vortices are dominant, while negatively rotating vortices are dominant on the right side of the jet. The characteristics of vortices, such as population density, average size and strength, and deviation velocity, were calculated and analyzed in both the cross-stream direction and the streamwise direction. In the near-field of the jet, the population density, average size and strength of the dominant direction vortices show high values on both sides of the center stream with a small number of counter-rotating vortices produced in the small wake regions close to jet outlet. As the flow develops, the wake regions disappear, these count-rotating vortices also disappear, and the population of the dominant direction vortices increase and spread in the jet. The mean size and strength of the vortices decrease monotonically with streamwise coordinate. The signs of vortex deviation velocity indicate the vortices transfer low momentum to high-velocity region and high momentum to the low velocity region. The developing trends of these characteristics were also identified by tracing vortices using time-resolved particle image velocimetry data. Both the mean tracked vortex strength and size decrease with increasing downstream distance overall. At the locations of the left peak of turbulent kinetic energy, the two-point spatial cross-correlation of swirling strength with velocity fluctuation and concentration fluctuation were calculated. All the correlation fields contain one positively correlated region and one negatively correlated region although the orientations of the correlation fields varied, due to the flow transitioning from wake, to jet, to channel flow. Finally, linear stochastic estimation was used to calculate conditional structures. The large

  10. Topological vortices in generalized Born-Infeld-Higgs electrodynamics

    Energy Technology Data Exchange (ETDEWEB)

    Casana, R. [Universidade Federal do Maranhao, Departamento de Fisica, Sao Luis, Maranhao (Brazil); Hora, E. da [Universidade Federal do Maranhao, Departamento de Fisica, Sao Luis, Maranhao (Brazil); Universidade Federal do Maranhao, Coordenadoria Interdisciplinar de Ciencia e Tecnologia, Sao Luis, Maranhao (Brazil); Rubiera-Garcia, D. [Fudan University, Department of Physics, Center for Field Theory and Particle Physics, Shanghai (China); Santos, C. dos [Faculdade de Ciencias da Universidade do Porto, Centro de Fisica e Departamento de Fisica e Astronomia, Porto (Portugal)


    A consistent BPS formalism to study the existence of topological axially symmetric vortices in generalized versions of the Born-Infeld-Higgs electrodynamics is implemented. Such a generalization modifies the field dynamics via the introduction of three nonnegative functions depending only in the Higgs field, namely,G(vertical stroke φ vertical stroke), w(vertical stroke φ vertical stroke), and V (vertical stroke φ vertical stroke). A set of first-order differential equations is attained when these functions satisfy a constraint related to the Ampere law. Such a constraint allows one to minimize the system's energy in such way that it becomes proportional to the magnetic flux. Our results provides an enhancement of the role of topological vortex solutions in Born-Infeld-Higgs electrodynamics. Finally, we analyze a set of models entailing the recovery of a generalized version of Maxwell-Higgs electrodynamics in a certain limit of the theory. (orig.)

  11. Driven phase space vortices in plasmas with nonextensive velocity distribution (United States)

    Trivedi, Pallavi; Ganesh, Rajaraman


    The evolution of chirp-driven electrostatic waves in unmagnetized plasmas is numerically investigated by using a one-dimensional (1D) Vlasov-poisson solver with periodic boundary conditions. The initial velocity distribution of the 1D plasma is assumed to be governed by nonextensive q distribution [C. Tsallis, J. Stat. Phys. 52, 479 (1988)]. For an infinitesimal amplitude of an external drive, we investigate the effects of chirp driven dynamics that leads to the formation of giant phase space vortices (PSV) for both Maxwellian (q = 1) and non-Maxwellian ( q ≠ 1 ) plasmas. For non-Maxwellian plasmas, the formation of giant PSV with multiple extrema and phase velocities is shown to be dependent on the strength of "q". Novel features such as "shark"-like and transient "honeycomb"-like structures in phase space are discussed. Wherever relevant, we compare our results with previous work.

  12. Vorticity Dynamics in Single and Multiple Swirling Reacting Jets (United States)

    Smith, Travis; Aguilar, Michael; Emerson, Benjamin; Noble, David; Lieuwen, Tim


    This presentation describes an analysis of the unsteady flow structures in two multinozzle swirling jet configurations. This work is motivated by the problem of combustion instabilities in premixed flames, a major concern in the development of modern low NOx combustors. The objective is to compare the unsteady flow structures in these two configurations for two separate geometries and determine how certain parameters, primarily distance between jets, influence the flow dynamics. The analysis aims to differentiate between the flow dynamics of single nozzle and triple nozzle configurations. This study looks at how the vorticity in the shear layers of one reacting swirling jet can affect the dynamics of a nearby similar jet. The distance between the swirling jets is found to have an effect on the flow field in determining where swirling jets merge and on the dynamics upstream of the merging location. Graduate Student, School of Aerospace Engineering, Georgia Institute of Technology, Atlanta, GA.

  13. Microalga propels along vorticity direction in a shear flow (United States)

    Chengala, Anwar; Hondzo, Miki; Sheng, Jian


    Using high-speed digital holographic microscopy and microfluidics, we discover that, when encountering fluid flow shear above a threshold, unicellular green alga Dunaliella primolecta migrates unambiguously in the cross-stream direction that is normal to the plane of shear and coincides with the local fluid flow vorticity. The flow shear drives motile microalgae to collectively migrate in a thin two-dimensional horizontal plane and consequently alters the spatial distribution of microalgal cells within a given suspension. This shear-induced algal migration differs substantially from periodic rotational motion of passive ellipsoids, known as Jeffery orbits, as well as gyrotaxis by bottom-heavy swimming microalgae in a shear flow due to the subtle interplay between torques generated by gravity and viscous shear. Our findings could facilitate mechanistic solutions for modeling planktonic thin layers and sustainable cultivation of microalgae for human nutrition and bioenergy feedstock.

  14. Resonance of Brownian vortices in viscoelastic shear flows (United States)

    Laas, K.; Mankin, R.


    The dynamics of a Brownian particle in an oscillatory viscoelastic shear flow is considered using the generalized Langevin equation. The interaction with fluctuations of environmental parameters is modeled by an additive external white noise and by an internal Mittag-Leffer noise with a finite memory time. Focusing on the mean angular momentum of particles it is shown that the presence of memory has a profound effect on the behavior of the Brownian vortices. Particularly, if an external noise dominates over the internal noise, a resonance-like dependence of the mean angular momentum of "free" particles, trapped due to the cage effect, on the characteristic memory time is observed. Moreover, it is established that memory effects can induce two kinds of resonance peaks: one resonance peak is related to the presence of external noise and the other is related to the initial positional distribution of particles. The bona fide resonance versus the shear frequency is also discussed.

  15. Role of electric discharges in the generation of atmospheric vortices (United States)

    Sinkevich, O. A.; Maslov, S. A.; Gusein-zade, N. G.


    The existing thermohydrodynamic and hydroelectromagnetic models of tornado are considered. The potentialities of the humid atmosphere as a heat engine generating air vortices are analyzed in detail. The ability of long-term atmospheric electric discharges to form a tornado funnel and create an initial twist of up to 10-3-10-2 s-1 in it are estimated. The possible effect of a lightning discharge on the initiation and evolution of the tornado is discussed. It is shown that the electric current flowing along the lightning channel can lead to helical instability and generation of a weak primary vortex. The channel formed in the atmosphere by a lightning discharge and the vortex motion of the parent thundercloud can enhance the primary vortex and promote its transformation into a tornado. Possible mechanisms of enhancement of the primary vortex created by a lightning discharge and the possibility of its transformation into a tornado in the postdischarge stage are discussed.

  16. Climate science in the tropics: waves, vortices and PDEs (United States)

    Khouider, Boualem; Majda, Andrew J.; Stechmann, Samuel N.


    Clouds in the tropics can organize the circulation on planetary scales and profoundly impact long range seasonal forecasting and climate on the entire globe, yet contemporary operational computer models are often deficient in representing these phenomena. On the other hand, contemporary observations reveal remarkably complex coherent waves and vortices in the tropics interacting across a bewildering range of scales from kilometers to ten thousand kilometers. This paper reviews the interdisciplinary contributions over the last decade through the modus operandi of applied mathematics to these important scientific problems. Novel physical phenomena, new multiscale equations, novel PDEs, and numerical algorithms are presented here with the goal of attracting mathematicians and physicists to this exciting research area.

  17. Promoter interference mediated by the U3 region in early-generation HIV-1-derived lentivirus vectors can influence detection of transgene expression in a cell-type and species-specific manner. (United States)

    Ginn, Samantha L; Fleming, Jane; Rowe, Peter B; Alexander, Ian E


    In a previous study using an early-generation VSV-G-pseudotyped lentivirus vector encoding enhanced green fluorescent protein (EGFP) under the transcriptional control of a human cytomegalovirus (CMV) immediate-early promoter, we examined transduction efficiency in dissociated dorsal root ganglia (DRG) cultures. In cultures of murine origin, transgene expression was observed solely in the sensory neurons with the stromal cell population failing to show evidence of transduction. In contrast, efficient and sustained transduction of both sensory neurons and the stromal cell population was observed in cultures of human origin. Given the widespread use of murine models in preclinical gene therapy studies, in the current study we investigated the basis of this apparent neuron specificity of lentivirus-mediated transduction in murine DRG cultures. The interspecies differences persisted at high multiplicities of infection, and irrespective of whether lentiviral vector stocks were packaged in the presence or absence of human immunodeficiency virus type 1 (HIV-1) accessory proteins. Cell-type specificity of CMV promoter expression, tropism of the VSV-G envelope, and blocks to molecular transduction were also precluded as possible mechanisms, thereby implicating transcriptional repression of the internal heterologous promoter. This promoter interference effect was found to be mediated by cis-acting sequences upstream of the core promoter elements located in the U3 region of the proviral long terminal repeats (LTRs). Deletion of this region, as in late-generation self-inactivating (SIN) lentivirus vectors, relieves this effect. This provides a basis for reevaluating data produced using early-generation U3-bearing lentivirus vectors and for reconciling these with results obtained using more contemporary SIN lentivirus vectors carrying a U3 deletion.

  18. HST and Keck AO Images of Vortices on Jupiter (United States)

    de Pater, Imke; Wong, M.; Luszcz-Cook, S.; Adamkovics, M.; Marcus, P.; Asay-Davis, X.; Conrad, A.; Go, C.


    Observations of Jupiter were conducted in April 2006 and May 2008 with both HST (250-890 nm) and the 10-m Keck telescope. On Keck we used the near-infrared camera NIRC2, coupled to the AO system, at 1.2 to 5 micrometers. At 5 um we are sensitive to Jupiter's thermal emission in cloud-free regions of the atmosphere, while at 1.2 um we see reflected sunlight from hazes and clouds in the troposphere-stratosphere. All small ovals (i.e., all except for Oval BA and the GRS) appear to be surrounded by bright rings at 5 mu, with typical brightness temperatures of 225-250 K, and hence probing depths to 2.5-4 bar. We argue that the small vortices are columnar structures, extending down into the water cloud. Air is rising along the center of these vortices, and descending around the outer periphery; the storm systems are likely fueled by the latent heat released from the water cloud. The descending part of the vertical circulation in the larger ovals is within the ovals, likely at the location of the red ring in Oval BA, which may indicate the maximum radial distance where such return flows can take place. On 19 July 2009, amateur observer Anthony Wesley (Australia) reported a dark spot/streak on Jupiter, near a southern latitude of 56 deg, which he attributed to an impact (analogous to the SL9 impact on Jupiter). We are scheduled to observe Jupiter with the Keck AO system on 24 July 2009 UT; if observations are successful, we will report preliminary results.

  19. History of Research on Lift-Generated Vortices (United States)

    Rossow, Vernon J.; Olson, Lawrence E. (Technical Monitor)


    A theoretical and experimental program on the structure and possible modification of lift-generated vortices shed by subsonic aircraft has been underway at NASA Ames Research Center since the late 1960's. The objectives of the program were to first obtain a better understanding of the hazard posed by the vortex wakes of subsonic transports, and then to develop methods on how to modify or design the wake-generating aircraft in order to make the vortices less hazardous. Some effort was also expended on the development of methods to avoid vortex wakes. The seminar will present a brief history of the research program beginning with the nature of vortex wakes and their relationship to the span loading on the wake-generating wing. Examples will then be presented to show how span-loading changes were used to influence the dynamics of the vortex wakes shed by subsonic transport aircraft. Video tape segments will be shown to illustrate the dynamics of vortex wakes and the flight tests carried out by NASA to find out how configurations developed in wind tunnels would perform on flight vehicles. The presentation will then summarize some recent results obtained in the 80- by 120-Foot Wind Tunnel at NASA Ames Research Center on the vortex wakes that trail from 0.03 scale models of a B-747 and of a DC-10 at downstream distances of 81 ft and 162 ft behind the wake-generating model; i.e., at scale distances of 0.5 and 1.0 mile. Lastly, a proposed vortex avoidance scheme based on the Global Positioning System will be described. All of the material to be presented is publically available in technical journals.

  20. Deep Coherent Vortices and Their Sea Surface Expressions (United States)

    Ienna, Federico; Bashmachnikov, Igor; Dias, Joaquim; Peliz, Alvaro


    Mediterranean Water eddies, known as Meddies, are an important dynamic process occurring at depths of 1000-meters in the Northeast Atlantic Ocean. Meddies occur as a direct result of the Mediterranean Outflow exiting through the Gibraltar Strait, and represent a prevalent mechanism that can be found extensively throughout the ocean. Moreover, Meddy cores are known to produce measurable expressions at the sea surface in the form of rotating coherent vortices, not only affecting the sea surface from beneath, but also allowing for the possibility to remotely study these deep phenomena through data gathered at the sea surface. While many past studies have focused on the properties of Meddy cores, only a handful of studies focus on the physical characteristics and behavior of the surface expressions produced. Are Meddy surface expressions different from other like vortices that dominate the physical ocean surface? What are the relationships between deep and surface mechanisms, and do any feedbacks exist? To shed light on these questions, we investigate the relationship between Meddies and their sea-surface expressions through observations using in-situ float and drifter profiles and satellite altimetry. A total of 782 Meddy cores were examined in the Northeast Atlantic using temperature and salinity data obtained by CTD and Argo during the Mecanismos de transporte e de dispersão da Água Mediterrânica no Atlântico Nordeste (MEDTRANS) project, and their corresponding sea-level expressions were geo-temporally matched in satellite altimetry data. We report several statistical properties of the sea-surface expressions of Meddies, including their mean diameter and vertical magnitude, and compare the properties of their surface features to the underlying Meddy cores. We investigate how the deep core affects the surface, and whether surface expressions may in return yield information about the underlying cores. Additionally, we examine the variability of the surface

  1. The "one-step" Bean pod mottle virus (BPMV)-derived vector is a functional genomics tool for efficient overexpression of heterologous protein, virus-induced gene silencing and genetic mapping of BPMV R-gene in common bean (Phaseolus vulgaris L.). (United States)

    Pflieger, Stéphanie; Blanchet, Sophie; Meziadi, Chouaib; Richard, Manon M S; Thareau, Vincent; Mary, Fanny; Mazoyer, Céline; Geffroy, Valérie


    Over the last two years, considerable advances have been made in common bean (Phaseolus vulgaris L.) genomics, especially with the completion of the genome sequence and the availability of RNAseq data. However, as common bean is recalcitrant to stable genetic transformation, much work remains to be done for the development of functional genomics tools adapted to large-scale studies. Here we report the successful implementation of an efficient viral vector system for foreign gene expression, virus-induced gene silencing (VIGS) and genetic mapping of a BPMV resistance gene in common bean, using a "one-step" BPMV vector originally developed in soybean. With the goal of developing this vector for high-throughput VIGS studies in common bean, we optimized the conditions for rub-inoculation of infectious BPMV-derived plasmids in common bean cv. Black Valentine. We then tested the susceptibility to BPMV of six cultivars, and found that only Black Valentine and JaloEEP558 were susceptible to BPMV. We used a BPMV-GFP construct to detect the spatial and temporal infection patterns of BPMV in vegetative and reproductive tissues. VIGS of the PHYTOENE DESATURASE (PvPDS) marker gene was successfully achieved with recombinant BPMV vectors carrying fragments ranging from 132 to 391 bp. Finally, we mapped a gene for resistance to BPMV (R-BPMV) at one end of linkage group 2, in the vicinity of a locus (I locus) previously shown to be involved in virus resistance. The "one-step" BPMV vector system therefore enables rapid and simple functional studies in common bean, and could be suitable for large-scale analyses. In the post-genomic era, these advances are timely for the common bean research community.

  2. The retrograde delivery of adenovirus vector carrying the gene for brain-derived neurotrophic factor protects neurons and oligodendrocytes from apoptosis in the chronically compressed spinal cord of twy/twy mice. (United States)

    Uchida, Kenzo; Nakajima, Hideaki; Hirai, Takayuki; Yayama, Takafumi; Chen, Kebing; Guerrero, Alexander Rodriguez; Johnson, William Eustace; Baba, Hisatoshi


    The twy/twy mouse undergoes spontaneous chronic mechanical compression of the spinal cord; this in vivo model system was used to examine the effects of retrograde adenovirus (adenoviral vector [AdV])-mediated brain-derived neurotrophic factor (BDNF) gene delivery to spinal neural cells. To investigate the targeting and potential neuroprotective effect of retrograde AdV-mediated BDNF gene transfection in the chronically compressed spinal cord in terms of prevention of apoptosis of neurons and oligodendrocytes. Several studies have investigated the neuroprotective effects of neurotrophins, including BDNF, in spinal cord injury. However, no report has described the effects of retrograde neurotrophic factor gene delivery in compressed spinal cords, including gene targeting and the potential to prevent neural cell apoptosis. AdV-BDNF or AdV-LacZ (as a control gene) was injected into the bilateral sternomastoid muscles of 18-week old twy/twy mice for retrograde gene delivery via the spinal accessory motor neurons. Heterozygous Institute of Cancer Research mice (+/twy), which do not undergo spontaneous spinal compression, were used as a control for the effects of such compression on gene delivery. The localization and cell specificity of β-galactosidase expression (produced by LacZ gene transfection) and BDNF expression in the spinal cord were examined by coimmunofluorescence staining for neural cell markers (NeuN, neurons; reactive immunology protein, oligodendrocytes; glial fibrillary acidic protein, astrocytes; OX-42, microglia) 4 weeks after gene injection. The possible neuroprotection afforded by retrograde AdV-BDNF gene delivery versus AdV-LacZ-transfected control mice was assessed by scoring the prevalence of apoptotic cells (terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling-positive cells) and immunoreactivity to active caspases -3, -8, and -9, p75, neurofilament 200 kD (NF), and for the oligodendroglial progenitor marker, NG2. RESULTS

  3. Vector mesons in matter

    Indian Academy of Sciences (India)

    One consequence of the chiral restoration is the mixing of parity partners. We look for a possible signature of the mixing of vector and axial vector mesons in heavy-ion collisions. We suggest an experimental method for its observation. The dynamical evolution of the heavy-ion collision is described by a transport equation of ...

  4. Vector mesons in matter

    Indian Academy of Sciences (India) Abstract. One consequence of the chiral restoration is the mixing of parity partners. We look for a possible signature of the mixing of vector and axial vector mesons in heavy- ion collisions. We suggest an experimental method for its ...

  5. Generating Series for Nested Bethe Vectors

    Directory of Open Access Journals (Sweden)

    Sergey Khoroshkin


    Full Text Available We reformulate nested relations between off-shell U_q(^gl_N Bethe vectors as a certain equation on generating series of strings of the composed U_q(^gl_N currents. Using inversion of the generating series we find a new type of hierarchical relations between universal off-shell Bethe vectors, useful for a derivation of Bethe equation. As an example of application, we use these relations for a derivation of analytical Bethe ansatz equations [Arnaudon D. et al., Ann. Henri Poincaré 7 (2006, 1217-1268, math-ph/0512037] for the parameters of universal Bethe vectors of the algebra U_q(^gl_2.

  6. Complex Polynomial Vector Fields

    DEFF Research Database (Denmark)

    Dias, Kealey

    The two branches of dynamical systems, continuous and discrete, correspond to the study of differential equations (vector fields) and iteration of mappings respectively. In holomorphic dynamics, the systems studied are restricted to those described by holomorphic (complex analytic) functions...... or meromorphic (allowing poles as singularities) functions. There already exists a well-developed theory for iterative holomorphic dynamical systems, and successful relations found between iteration theory and flows of vector fields have been one of the main motivations for the recent interest in holomorphic...... vector fields. Since the class of complex polynomial vector fields in the plane is natural to consider, it is remarkable that its study has only begun very recently. There are numerous fundamental questions that are still open, both in the general classification of these vector fields, the decomposition...

  7. Arabic Text Classification Using Support Vector Machines

    NARCIS (Netherlands)

    Gharib, Tarek F.; Habib, Mena B.; Fayed, Zaki T.


    Text classification (TC) is the process of classifying documents into a predefined set of categories based on their content. Arabic language is highly inflectional and derivational language which makes text mining a complex task. In this paper we applied the Support Vector Machines (SVM) model in

  8. Fractional vector calculus for fractional advection dispersion (United States)

    Meerschaert, Mark M.; Mortensen, Jeff; Wheatcraft, Stephen W.


    We develop the basic tools of fractional vector calculus including a fractional derivative version of the gradient, divergence, and curl, and a fractional divergence theorem and Stokes theorem. These basic tools are then applied to provide a physical explanation for the fractional advection-dispersion equation for flow in heterogeneous porous media.

  9. Unified Analysis of Multi-Chamber Contact Tanks and Mixing Efficiency Based on Vorticity Field. Part I: Hydrodynamic Analysis

    Directory of Open Access Journals (Sweden)

    Ender Demirel


    Full Text Available Multi-chamber contact tanks have been extensively used in industry for water treatment to provide potable water to communities, which is essential for human health. To evaluate the efficiency of this treatment process, flow and tracer transport analysis have been used in the literature using Reynolds averaged Navier–Stokes (RANS and large-eddy simulations (LES. The purpose of this study is two-fold. First a unifying analysis of the flow field is presented and similarities and differences in the numerical results that were reported in the literature are discussed. Second, the vorticity field is identified as the key parameter to use in separating the mean flow (jet zone and the recirculating zones. Based on the concepts of vorticity gradient and flexion product, it is demonstrated that the separation of the recirculation zone and the jet zone, fluid-fluid flow separation, is possible. The separation of the recirculation zones and vortex core lines are characterized using the definition of the Lamb vector. The separated regions are used to characterize the mixing efficiency in the chambers of the contact tank. This analysis indicates that the recirculation zone and jet zone formation are three-dimensional and require simulations over a long period of time to reach stability. It is recognized that the characteristics of the jet zones and the recirculation zones are distinct for each chamber and they follow a particular pattern and symmetry between the alternating chambers. Hydraulic efficiency coefficients calculated for each chamber show that the chambers having an inlet adjacent to the free surface may be designed to have larger volumes than the chambers having wall bounded inlets to improve the efficiency of the contact tank. This is a simple design alternative that would increase the efficiency of the system. Other observations made through the chamber analysis are also informative in redefining the characteristics of the efficiency of the

  10. Towards laboratory detection of topological vortices in superfluid phases of QCD (United States)

    Das, Arpan; Dave, Shreyansh S.; de, Somnath; Srivastava, Ajit M.


    Topological defects arise in a variety of systems, e.g. vortices in superfluid helium to cosmic strings in the early universe. There is an indirect evidence of neutron superfluid vortices from the glitches in pulsars. One also expects that the topological defects may arise in various high baryon density phases of quantum chromodynamics (QCD), e.g. superfluid topological vortices in the color flavor locked (CFL) phase. Though vastly different in energy/length scales, there are universal features in the formation of all these defects. Utilizing this universality, we investigate the possibility of detecting these topological superfluid vortices in laboratory experiments, namely heavy-ion collisions (HICs). Using hydrodynamic simulations, we show that vortices can qualitatively affect the power spectrum of flow fluctuations. This can give an unambiguous signal for superfluid transition resulting in vortices, allowing for the check of defect formation theories in a relativistic quantum field theory system, and the detection of superfluid phases of QCD. Detection of nucleonic superfluid vortices in low energy HICs will give opportunity for laboratory controlled study of their properties, providing crucial inputs for the physics of pulsars.

  11. Motion and interaction of decaying trailing vortices in spanwise shear wind (United States)

    Liu, C. H.; Lu, T.


    A simulation is presented of the drift of trailing vortices in a cross-wind near the ground by an unsteady, two-dimensional, rotational flow field with a concentration of large vorticity in vortical spots (having a finite but small effective size and finite total strength). The problem is analyzed by a combination of the method of matched asymptotic analyses for the decay of the vortical spots and the Euler solution for the unsteady rotational flow. Using the method of averaging, a special numerical method is developed in which the grid size and time step depend only on the length and velocity scales of the background flow and are independent of the effective core size of a vortical spot. The core size can be much smaller than the grid size, whereas the peak velocity in the core is inversely propertional to the spot size. Numerical results are presented to demonstrate the strong interaction between the trajectories of the vortical spots and the change of the vorticity distribution in the background flow field.

  12. Polar vortices on Earth and Mars: A comparative study of the climatology and variability from reanalyses. (United States)

    Mitchell, D M; Montabone, L; Thomson, S; Read, P L


    Polar vortices on Mars provide case-studies to aid understanding of geophysical vortex dynamics and may help to resolve long-standing issues regarding polar vortices on Earth. Due to the recent development of the first publicly available Martian reanalysis dataset (MACDA), for the first time we are able to characterise thoroughly the structure and evolution of the Martian polar vortices, and hence perform a systematic comparison with the polar vortices on Earth. The winter atmospheric circulations of the two planets are compared, with a specific focus on the structure and evolution of the polar vortices. The Martian residual meridional overturning circulation is found to be very similar to the stratospheric residual circulation on Earth during winter. While on Earth this residual circulation is very different from the Eulerian circulation, on Mars it is found to be very similar. Unlike on Earth, it is found that the Martian polar vortices are annular, and that the Northern Hemisphere vortex is far stronger than its southern counterpart. While winter hemisphere differences in vortex strength are also reported on Earth, the contrast is not as large. Distinctions between the two planets are also apparent in terms of the climatological vertical structure of the vortices, in that the Martian polar vortices are observed to decrease in size at higher altitudes, whereas on Earth the opposite is observed. Finally, it is found that the Martian vortices are less variable through the winter than on Earth, especially in terms of the vortex geometry. During one particular major regional dust storm on Mars (Martian year 26), an equatorward displacement of the vortex is observed, sharing some qualitative characteristics of sudden stratospheric warmings on Earth.

  13. Vector SIMP dark matter (United States)

    Choi, Soo-Min; Hochberg, Yonit; Kuflik, Eric; Lee, Hyun Min; Mambrini, Yann; Murayama, Hitoshi; Pierre, Mathias


    Strongly Interacting Massive Particles (SIMPs) have recently been proposed as light thermal dark matter relics. Here we consider an explicit realization of the SIMP mechanism in the form of vector SIMPs arising from an SU(2) X hidden gauge theory, where the accidental custodial symmetry protects the stability of the dark matter. We propose several ways of equilibrating the dark and visible sectors in this setup. In particular, we show that a light dark Higgs portal can maintain thermal equilibrium between the two sectors, as can a massive dark vector portal with its generalized Chern-Simons couplings to the vector SIMPs, all while remaining consistent with experimental constraints.

  14. Vector Difference Calculus (United States)

    Schwalm, W. A.; Schwalm, M. K.; Giona, M.


    Space is filled with triangulating graph \\calG to serve as a quadrature grid. A discrete analog of the theory of differential forms is constructed using the associated simplical complex. The role of a basis for Λ^p at a point is played by the set of (p+1) -simplices containing a given vertex. Vector difference operations analogous to div, grad and curl, together with corresponding vector identities and exact difference analogs of the Stokes-type theorems, are obtained in terms of the boundary partial and coboundary d. Difference versions of the full vector Maxwell electromagnetic equations are analyzed on a random structure.

  15. LandSat-Based Land Use-Land Cover (Vector) (United States)

    Minnesota Department of Natural Resources — Vector-based land cover data set derived from classified 30 meter resolution Thematic Mapper satellite imagery. Classification is divided into 16 classes with source...

  16. Gyroscopic dynamics of antiferromagnetic vortices on domain boundaries of yttrium orthoferrite

    CERN Document Server

    Chetkin, M V; Shapaeva, T B


    It is experimentally established that the magnetic field along the b-axis weakly effects the velocity of the antiferromagnetic vortices on the domain boundary of the yttrium orthoferrite and doesn't explain the existence of the essential gyroscopic force, effecting these vortices. The existence of such a force is caused by the dynamic slope of the magnetic sublattices, proportional to the domain boundary velocity. The dynamics of the antiferromagnetic vortices in the yttrium orthoferrite domain boundaries is gyroscopic and quasirelativistic one with the threshold velocity of 20 km/s, equal to the spin waves velocity on the linear section of their dispersion law

  17. CFD prediction and model experiment on suction vortices in pump sump


    Okamura, Tomoyoshi; Kamemoto, Kyoji; Matsui, Jun


    The sump size is being reduced in order to lower the construction costs of urban drainage pump stations in Japan. As a result of such size reductions, undesirable vortices such as air-entrained and submerged vortices are apt to appear in sumps because of the higher flow velocities. The Turbomachinery Society of Japan (TSJ) Standard S002:2005 states that the appearance of such visible vortices is not permissible for conventional sumps, and experiments with scale models usually have been done t...

  18. Black holes in vector-tensor theories (United States)

    Heisenberg, Lavinia; Kase, Ryotaro; Minamitsuji, Masato; Tsujikawa, Shinji


    We study static and spherically symmetric black hole (BH) solutions in second-order generalized Proca theories with nonminimal vector field derivative couplings to the Ricci scalar, the Einstein tensor, and the double dual Riemann tensor. We find concrete Lagrangians which give rise to exact BH solutions by imposing two conditions of the two identical metric components and the constant norm of the vector field. These exact solutions are described by either Reissner-Nordström (RN), stealth Schwarzschild, or extremal RN solutions with a non-trivial longitudinal mode of the vector field. We then numerically construct BH solutions without imposing these conditions. For cubic and quartic Lagrangians with power-law couplings which encompass vector Galileons as the specific cases, we show the existence of BH solutions with the difference between two non-trivial metric components. The quintic-order power-law couplings do not give rise to non-trivial BH solutions regular throughout the horizon exterior. The sixth-order and intrinsic vector-mode couplings can lead to BH solutions with a secondary hair. For all the solutions, the vector field is regular at least at the future or past horizon. The deviation from General Relativity induced by the Proca hair can be potentially tested by future measurements of gravitational waves in the nonlinear regime of gravity.

  19. Spontaneous formation of quantized vortices in Bose-Einstein condensates (United States)

    Weiler, Chad Nathan

    Phase transitions abound in the physical world, from the subatomic length scales of quark condensation to the decoupling forces in the early universe. In the Bose-Einstein condensation phase transition, a gas of trapped bosonic atoms is cooled to a critical temperature. Below this temperature, a macroscopic number of atoms suddenly starts to occupy a single quantum state; these atoms comprise the Bose-Einstein condensate (BEC). The dynamics of the BEC phase transition are the focus of this dissertation and the experiments described here have provided new information on the details of BEC formation. New theoretical developments are proving to be valuable tools for describing BEC phase transition dynamics and interpreting new experimental results. With their amenability to optical manipulation and probing along with the advent of new microscopic theories, BECs provide an important new avenue for gaining insight into the universal dynamics of phase transitions in general. Spontaneous symmetry breaking in the system's order parameter may be one result of cooling through a phase transition. A potential consequence of this is the spontaneous formation of topological defects, which in a BEC appear as vortices. We experimentally observed and characterized the spontaneous formation of vortices during BEC growth. We attribute vortex creation to coherence length limitations during the initial stages of the phase transition. Parallel to these experimental observations, theory collaborators have used the Stochastic Gross-Pitaevski Equation formalism to simulate the growth of a condensate from a thermal cloud. The experimental and theoretical statistical results of the spontaneous formation of vortex cores during the growth of the condensate are in good quantitative agreement with one another, supporting our understanding of the dynamics of the phase transition. We believe that our results are also qualitatively consistent with the Kibble-Zurek mechanism, a universal model for

  20. Retroflection from slanted coastlines-circumventing the "vorticity paradox"

    Directory of Open Access Journals (Sweden)

    V. Zharkov


    Full Text Available The balance of long-shore momentum flux requires that the solution of zonally retroflecting currents involve ring shedding on the western side. An important aspect of the ring dynamics is the ring intensity α (analogous to the Rossby number, which reaches its maximum value of unity when the upstream potential vorticity (PV is zero. Friction leads to a slow-down and a decrease in α. The main difficulty is that the solution of the system of equations for conservation of mass and momentum of zonal currents leads to the conclusion that the ratio (Φ of the mass flux going into the rings and the total incoming mass flux is approximately 4α/(1+2α. This yields the "vorticity paradox" – only relatively weak rings (α≤1/2 could satisfy the necessary condition Φ≤1. Physically, this means, for example, that the momentum-flux of zero PV currents upstream is so high that, no matter how many rings are produced and, no matter what size they are, they cannot compensate for it.

    To avoid this paradox, we develop a nonlinear analytical model of retroflection from a slanted non-zonal coastline. We show that when the slant of coastline (γ exceeds merely 150, Φ does not reach unity regardless of the value of α. Namely, the paradox disappears even for small slants. Our slowly varying nonlinear solution does not only let us circumvent the paradox. It also gives a detailed description of the rings growth rate and the mass flux going into the rings as a function of time. For example, in the case of zero PV and zero thickness of the upper layer along the coastline, the maximal values of Φ can be approximately expressed as, 1.012+0.32exp(−γ/3.41−γ/225. Interestingly, for significant slants γ≥300, the rings reach a terminal size corresponding to a balance between the β-force and both the upstream and downstream momentum fluxes. This terminal size is unrelated to the ultimate

  1. Application of Bred Vectors To Data Assimilation (United States)

    Corazza, M.; Kalnay, E.; Patil, Dj

    ,0,0]=1.8, less than 2 because one direction is more dominant than the other in representing the original data. The results (Patil et al, 2001) show that there are large regions where the bred vectors span a subspace of substantially lower dimension than that of the full space. These low dimensionality regions are dominant in the baroclinic extratropics, typically have a lifetime of 3-7 days, have a well-defined horizontal and vertical structure that spans 1 most of the atmosphere, and tend to move eastward. New results with a large number of ensemble members confirm these results and indicate that the low dimensionality regions are quite robust, and depend only on the verification time (i.e., the underlying flow). Corazza et al (2001) have performed experiments with a data assimilation system based on a quasi-geostrophic model and simulated observations (Morss, 1999, Hamill et al, 2000). A 3D-variational data assimilation scheme for a quasi-geostrophic chan- nel model is used to study the structure of the background error and its relationship to the corresponding bred vectors. The "true" evolution of the model atmosphere is defined by an integration of the model and "rawinsonde observations" are simulated by randomly perturbing the true state at fixed locations. It is found that after 3-5 days the bred vectors develop well organized structures which are very similar for the two different norms considered in this paper (potential vorticity norm and streamfunction norm). The results show that the bred vectors do indeed represent well the characteristics of the data assimilation forecast errors, and that the subspace of bred vectors contains most of the forecast error, except in areas where the forecast errors are small. For example, the angle between the 6hr forecast error and the subspace spanned by 10 bred vectors is less than 10o over 90% of the domain, indicating a pattern correlation of more than 98.5% between the forecast error and its projection onto the bred vector

  2. GAP Land Cover - Vector (United States)

    Minnesota Department of Natural Resources — This vector dataset is a detailed (1-acre minimum), hierarchically organized vegetation cover map produced by computer classification of combined two-season pairs of...

  3. Tagged Vector Contour (TVC) (United States)

    Kansas Data Access and Support Center — The Kansas Tagged Vector Contour (TVC) dataset consists of digitized contours from the 7.5 minute topographic quadrangle maps. Coverage for the state is incomplete....

  4. Analysis of Finite Element Methods for Vector Laplacians on Surfaces


    Hansbo, Peter; Larson, Mats G.; Larsson, Karl


    We develop a finite element method for the vector Laplacian based on the covariant derivative of tangential vector fields on surfaces embedded in $\\mathbb{R}^3$. Closely related operators arise in models of flow on surfaces as well as elastic membranes and shells. The method is based on standard continuous parametric Lagrange elements with one order higher polynomial degree for the mapping. The tangent condition is weakly enforced using a penalization term. We derive error estimates that take...

  5. Vorticity analysis of transient shallow water eddy fields at the river plume front of the River Elbe in the German Bight (United States)

    Dippner, Joachim W.


    The mesoscale variability of the circulation of the German Bight is investigated for selected wind forcing situations with an eddy resolving model. A vorticity equation is derived which considers explicitly both mass distribution and tides. Different shallow water eddy regimes are identified in the German Bight. Using the vorticity equation, the statistical steady state eddy fields are analyzed with respect to the production mechanisms of the gyres and shallow water eddies. The large German Bight gyre is affected by the joint effect of baroclinicity and topography. It is demonstrated that all shallow water eddies which appear at the East-Friesian coast are affected by the interaction between wind stress and the gradient of bottom topography. Small shallow water eddies at the North-Friesian coast formed from the baroclinic forcing, the vortex squeezing and the interaction between wind stress and the gradient of bottom topography. The results further suggest that the contribution of advection or of tidal stress to the vorticity production is of minor relevance.

  6. Co-occurrence Vectors from Corpora vs. Distance Vectors from Dictionaries

    CERN Document Server

    Niwa, Y; Niwa, Yoshiki; Nitta, Yoshihiko


    A comparison was made of vectors derived by using ordinary co-occurrence statistics from large text corpora and of vectors derived by measuring the inter-word distances in dictionary definitions. The precision of word sense disambiguation by using co-occurrence vectors from the 1987 Wall Street Journal (20M total words) was higher than that by using distance vectors from the Collins English Dictionary (60K head words + 1.6M definition words). However, other experimental results suggest that distance vectors contain some different semantic information from co-occurrence vectors. From Fri Nov 12 12:25 MET 1999 Received: from ( []) by (8.8.5/8.8.5) with ESMTP id MAA10923 for ; Fri, 12 Nov 1999 12:25:47 +0100 (MET) Received: from ( []) by (8.9.3/8.9.3) with ESMTP id MAA18850 for ; Fri, 12 Nov 1999 12:25:46 +0100 (MET) Received: from ( []) by uu...

  7. Syngeneic AAV pseudo-vectors potentiates full vector transduction (United States)

    An excessive amount of empty capsids are generated during regular AAV vector production process. These pseudo-vectors often remain in final vectors used for animal studies or clinical trials. The potential effects of these pseudo-vectors on AAV transduction have been a major concern. In the current ...

  8. Vector and axial vector mesons at finite temperature


    mallik, S.; Sarkar, Sourav


    We consider the thermal correlation functions of vector and axial-vector currents and evaluate corrections to the vector and axial-vector meson pole terms to one loop in chiral perturbation theory. As expected, the pole positions do not shift to leading order in temperature. But the residues decrease with temperature.

  9. Vortices generation in the reactive flow on the evaporative surface

    Energy Technology Data Exchange (ETDEWEB)

    Park, Cha Ryeom; Lee, Chang Jin [Konkuk University, Seoul (Korea, Republic of)


    Vortices generation and flow dynamics are investigated by a numerical calculation with LES methodology on the evaporative surface including chemical reactions. For simplicity, fuel is radially injected from the surface in order to decouple pyrolysis of solid fuel from the governing equation and consideration of heat transfer balance. Nevertheless its simple treatment of chemical reactions and fuel pyrolysis, numerical results captured very fundamental understandings in terms of averaged temperature, velocity profile, and mixture fraction distribution. Results showed that a well-defined turbulent velocity profile at the inlet becomes twisted and highly wrinkled in the downstream reaching the maximum velocity at far above the surface, where the flame is located. And the thickness of boundary layer increases in the downstream due to the enhanced interaction of axial flow and mass injection from the surface. Also, chemical reaction appears highly active and partially concentrated along the plane where flow condition is in stoichiometric. In particular, flame front locates at the surface where mixture fraction Z equals to 0.07. Flame front severely wrinkles in the downstream by the interaction with turbulences in the flow. Partial reactions on the flame front contribute to produce hot spots periodically in the downstream attaining the max temperature at the center of each spot. This may take the role of additional unsteady heat generations and pressure perturbations in the downstream. Future study will focus on the evolution of hot spots and pressure perturbations in the post chamber of lab scale hybrid rocket motors.

  10. Evolution of vorticity and enstrophy in the intracluster medium (United States)

    Wittor, D.; Jones, T.; Vazza, F.; Brüggen, M.


    Turbulence generated by large-scale motions during structure formation affects the evolution of the thermal and non-thermal components of the intracluster medium. As enstrophy is a measure of the magnitude of vorticity, we study the generation and evolution of turbulence by analysing the Lagrangian history of enstrophy. For this purpose, we combine cosmological simulations carried out with the enzo code with our Lagrangian post-processing tool CRaTer. This way we are able to quantify the individual source terms of enstrophy in the course of the accretion of groups on to galaxy clusters. Here, we focus on the redshift range from z = 1 to z = 0. Finally, we measure the rate of dissipation of turbulence and estimate the resulting amplification of intracluster magnetic fields. We find that compressive and baroclinic motions are the main sources of enstrophy, while stretching motions and dissipation affect most of the ensuing enstrophy evolution. The rate of turbulent dissipation is able to sustain the amplification of intracluster magnetic fields to observed levels.

  11. Role of electric discharges in the generation of atmospheric vortices

    Energy Technology Data Exchange (ETDEWEB)

    Sinkevich, O. A., E-mail: [National Research University “MPEI,” (Russian Federation); Maslov, S. A., E-mail: [Russian Academy of Sciences, Joint Institute for High Temperatures (Russian Federation); Gusein-zade, N. G., E-mail: [Russian Academy of Sciences, Prokhorov General Physics Institute (Russian Federation)


    The existing thermohydrodynamic and hydroelectromagnetic models of tornado are considered. The potentialities of the humid atmosphere as a heat engine generating air vortices are analyzed in detail. The ability of long-term atmospheric electric discharges to form a tornado funnel and create an initial twist of up to 10{sup –3}–10{sup –2} s{sup –1} in it are estimated. The possible effect of a lightning discharge on the initiation and evolution of the tornado is discussed. It is shown that the electric current flowing along the lightning channel can lead to helical instability and generation of a weak primary vortex. The channel formed in the atmosphere by a lightning discharge and the vortex motion of the parent thundercloud can enhance the primary vortex and promote its transformation into a tornado. Possible mechanisms of enhancement of the primary vortex created by a lightning discharge and the possibility of its transformation into a tornado in the postdischarge stage are discussed.

  12. Hawkmoth flight performance in tornado-like whirlwind vortices. (United States)

    Ortega-Jimenez, Victor Manuel; Mittal, Rajat; Hedrick, Tyson L


    Vertical vortex systems such as tornadoes dramatically affect the flight control and stability of aircraft. However, the control implications of smaller scale vertically oriented vortex systems for small fliers such as animals or micro-air vehicles are unknown. Here we examined the flapping kinematics and body dynamics of hawkmoths performing hovering flights (controls) and maintaining position in three different whirlwind intensities with transverse horizontal velocities of 0.7, 0.9 and 1.2 m s(-1), respectively, generated in a vortex chamber. The average and standard deviation of yaw and pitch were respectively increased and reduced in comparison with hovering flights. Average roll orientation was unchanged in whirlwind flights but was more variable from wingbeat to wingbeat than in hovering. Flapping frequency remained unchanged. Wingbeat amplitude was lower and the average stroke plane angle was higher. Asymmetry was found in the angle of attack between right and left wings during both downstroke and upstroke at medium and high vortex intensities. Thus, hawkmoth flight control in tornado-like vortices is achieved by a suite of asymmetric and symmetric changes to wingbeat amplitude, stroke plane angle and principally angle of attack.

  13. Transport and potential vorticity in the Bay of Bengal during the southwest monsoon

    Digital Repository Service at National Institute of Oceanography (India)

    Murty, V.S.N.; Murty, C.S.; Sarma, Y.V.B.; Rao, D.P.; Sastry, J.S.; Rao, G.R.L.

    In the Bay of Bengal, the water transport and potential vorticity (PV) during the southwest monsoon are examined through the prevailing thermohaline and wind-driven circulation. The Indian Monsoon Current (IMC) and the north flowing Eastern Boundary...

  14. Deterministic creation, pinning, and manipulation of quantized vortices in a Bose-Einstein condensate (United States)

    Samson, E. C.; Wilson, K. E.; Newman, Z. L.; Anderson, B. P.


    We experimentally and numerically demonstrate deterministic creation and manipulation of a pair of oppositely charged singly quantized vortices in a highly oblate Bose-Einstein condensate (BEC). Two identical blue-detuned, focused Gaussian laser beams that pierce the BEC serve as repulsive obstacles for the superfluid atomic gas; by controlling the positions of the beams within the plane of the BEC, superfluid flow is deterministically established around each beam such that two vortices of opposite circulation are generated by the motion of the beams, with each vortex pinned to the in situ position of a laser beam. We study the vortex creation process, and show that the vortices can be moved about within the BEC by translating the positions of the laser beams. This technique can serve as a building block in future experimental techniques to create, on-demand, deterministic arrangements of few or many vortices within a BEC for precise studies of vortex dynamics and vortex interactions.

  15. A quantitative comparison of leading-edge vortices in incompressible and supersonic flows (United States)


    When requiring quantitative data on delta-wing vortices for design purposes, low-speed results have often been extrapolated to configurations intended for supersonic operation. This practice stems from a lack of database owing to difficulties that pl...

  16. Decay of MHD-scale Kelvin-Helmholtz vortices mediated by parasitic electron dynamics. (United States)

    Nakamura, T K M; Hayashi, D; Fujimoto, M; Shinohara, I


    We have simulated nonlinear development of MHD-scale Kelvin-Helmholtz (KH) vortices by a two-dimensional two-fluid system including finite electron inertial effects. In the presence of moderate density jump across a shear layer, in striking contrast to MHD results, MHD KH vortices are found to decay by the time one eddy turnover is completed. The decay is mediated by smaller vortices that appear within the parent vortex and stays effective even when the shear layer width is made larger. It is shown that the smaller vortices are basically of MHD nature while the seeding for these is achieved by the electron inertial effect. Application of the results to the magnetotail boundary layer is discussed.

  17. Potential Vorticity based parameterization for specification of Upper troposphere/lower stratosphere ozone in atmospheric models (United States)

    U.S. Environmental Protection Agency — Potential Vorticity based parameterization for specification of Upper troposphere/lower stratosphere ozone in atmospheric models - the data set consists of 3D O3...

  18. Observation of Half-Quantum Vortices in an Exciton-Polariton Condensate

    National Research Council Canada - National Science Library

    K. G. Lagoudakis; T. Ostatnický; A. V. Kavokin; Y. G. Rubo; R. André; B. Deveaud-Plédran


    Singly quantized vortices have already been observed in many systems, including the superfluid helium, Bose-Einstein condensates of dilute atomic gases, and condensates of exciton-polaritons in the solid state...

  19. On periodic geophysical water flows with discontinuous vorticity in the equatorial f-plane approximation (United States)

    Martin, Calin Iulian


    We are concerned here with geophysical water waves arising as the free surface of water flows governed by the f-plane approximation. Allowing for an arbitrary bounded discontinuous vorticity, we prove the existence of steady periodic two-dimensional waves of small amplitude. We illustrate the local bifurcation result by means of an analysis of the dispersion relation for a two-layered fluid consisting of a layer of constant non-zero vorticity γ1 adjacent to the surface situated above another layer of constant non-zero vorticity γ2≠γ1 adjacent to the bed. For certain vorticities γ1,γ2, we also provide estimates for the wave speed c in terms of the speed at the surface of the bifurcation inducing laminar flows. This article is part of the theme issue 'Nonlinear water waves'.

  20. Structure, stability, and evolution of 3D Rossby vortices in protoplanetary disks (United States)

    Richard, S.; Barge, P.; Le Dizès, S.


    Context. Large-scale persistent vortices could play a key role in the evolution of protoplanetary disks, particularly in the dead zone where no turbulence associated with a magnetic field is expected. These vortices are known to form easily in 2D disks via the Rossby wave or the baroclinic instability. In three dimensions, however, their formation and stability is a complex problem and still a matter of debate. Aims: We study the formation of vortices by the Rossby wave instability in a stratified inviscid disk and describe their 3D structure, stability, and long-term evolution. Methods: Numerical simulations were performed using a fully compressible hydrodynamical code based on a second-order finite volume method. We assumed a perfect-gas law and a non-homentropic adiabatic flow. Results: The Rossby wave instability is found to proceed in 3D in a similar way as in 2D. Vortices produced by the instability look like columns of vorticity in the whole disk thickness; the weak vertical motions are related to the weak inclination of the vortex axis that appears during the development of the RWI. Vortices with aspect ratios higher than 6 are unaffected by the elliptical instability. They relax into a quasi-steady columnar structure that survives hundreds of rotations while slowly migrating inward toward the star at a rate that reduces with the vortex aspect ratio. Vortices with a lower aspect ratio are by contrast affected by the elliptic instability. Short aspect ratio vortices (χ < 4) are completely destroyed in a few orbital periods. Vortices with an intermediate aspect ratio (4 < χ < 6) are partially destroyed by the elliptical instability in a region away from the midplane where the disk stratification is sufficiently strong. Conclusions: Elongated Rossby vortices can survive many orbital periods in protoplanetary disks in the form of vorticity columns. They could play a significant role in the evolution of the gas and the gathering of solid particles to form

  1. Direct Assessment of Vorticity Alignment with Local and Nonlocal Strain Rates in Turbulent Flows


    Hamlington, Peter E.; Schumacher, Jörg; Dahm, Werner J. A.


    A direct Biot-Savart integration is used to decompose the strain rate into its local and nonlocal constituents, allowing the vorticity alignment with the local and nonlocal strain rate eigenvectors to be investigated. These strain rate tensor constituents are evaluated in a turbulent flow using data from highly-resolved direct numerical simulations. While the vorticity aligns preferentially with the intermediate eigenvector of the \\textit{combined} strain rate, as has been observed previously...

  2. The interplay of curvature and vortices in flow on curved surfaces


    Reuther, Sebastian; Voigt, Axel


    Incompressible fluids on curved surfaces are considered with respect to the interplay between topology, geometry and fluid properties using a surface vorticity-stream function formulation, which is solved using parametric finite elements. Motivated by designed examples for superfluids, we consider the influence of a geometric potential on vortices for fluids with finite viscosity and show numerical examples in which a change in the geometry is used to manipulate the flow field.

  3. Fluctuating pancake vortices revealed by dissipation of the Josephson vortex lattice


    Koshelev, A.E.; Buzdin, A. I.; Kakeya, I.; T. Yamamoto; Kadowaki, K


    In strongly anisotropic layered superconductors in tilted magnetic fields, the Josephson vortex lattice coexists with the lattice of pancake vortices. Due to the interaction between them, the dissipation of the Josephson vortex lattice is very sensitive to the presence of the pancake vortices. If the c-axis magnetic field is smaller than the corresponding lower critical field, the pancake stacks are not formed but the individual pancakes may exist in the fluctuational regime either near the s...

  4. Experimental investigation of local properties and statistics of optical vortices in random wave fields

    DEFF Research Database (Denmark)

    Wang, W.; Hanson, Steen Grüner; Miyamoto, Y.


    We present the first direct experimental evidence of the local properties of optical vortices in a random laser speckle field. We have observed the Berry anisotropy ellipse describing the anisotropic squeezing of phase lines close to vortex cores and quantitatively verified the Dennis angular mom...... momentum rule for its phase. Some statistics associated with vortices, such as density, anisotropy ellipse eccentricity, and its relation to zero crossings of real and imaginary parts of the random field, are also investigated by experiments....

  5. Vorticity visualization: phantom study for a new discriminant parameter in US elastography (United States)

    Sosa-Cabrera, D.; Rodriguez-Florido, M. A.; Suarez-Santana, E.; Ruiz-Alzola, Juan


    Ultrasound elastography measures the elastic properties of soft tissues using ultrasound signals. The elastic problem can be analyzed with tensor signal processing. In this work, we propose a new interpretation of elastography through the deformation tensor and its decomposition into both the strain and vorticity tensors. Vorticity gives information about the rotation of the inclusions that might help in the discrimination between malign and benign tumors without using biopsy. Although clinical validation is needed, synthetic experiments present reliable results.

  6. On the relation of 6.7-micron water vapour features to isentropic distributions of potential vorticity (United States)

    Manney, Gloria L.; Stanford, John L.


    Currently available isentropic maps of potential vorticity (IPV maps) are of low resolution. In this note, the possibility of using 6.7-micron satellite-derived water vapor measurements to obtain higher resolution information about IPV distributions is examined. While attempts to related the two data sets analytically have so far proved unsuccessful, a qualitative comparison shows very similar features in both. The results obtained here suggest that, over limited areas, it may be possible to use 6.7-micron brightness temperatures to obtain approximate contours for water vapor mixing ratio on a particular isentropic surface in the upper troposphere. For areas free of high clouds, these contours are expected to parallel the IPV contours on this surface. In a case study, the resulting map exhibits dramatically increased horizontal resolution compared with present IPV maps, detailing an intriguing upper-tropospheric feature at subsynoptic scales.

  7. On the forced tangentially-averaged radial-vertical circulation within vortices part I: Concepts and equations (United States)

    Zhuojian, Yuan; Maoqiu, Jian


    A linear partial differential equation is derived in cylindrical-isobaric coordinates on the earth for the diagnostic study of the tangentially-averaged radial-vertical circulation within translating vortices. In the hydrodynamic stable atmosphere, the circulation will be forced through many dynamic and thermodynamic processes. These processes are associated with frictional torque, inertial torque, the horizontal and vertical divergence of eddy angular momentum, diabatic heating, adiabatic heating, and eddy temperature advection. For a given forcing, the intensity of circulation will increase with the decrease of static, inertial, and baroclinic stabilities. This paper also presents an explanation on the data interpolation from the latitude-longitude grid to the vortex volume gird and a brief discussion on the forcing processes.

  8. Mechanisms, role of vorticity, and time scales for planar liquid sheet breakup (United States)

    Zandian, Arash; Sirignano, William; Hussain, Fazle


    The 3D, temporal instabilities on a planar liquid sheet are studied using DNS with level-set and VoF surface tracking methods. λ2 contours relate the vorticity to the surface dynamics. The breakup character depends on the Ohnesorge number (Oh). At high Oh , hairpin vortices form on the braid and overlap with the lobe hairpins, thinning the lobes, which puncture creating holes and bridges. The bridges break, creating ligaments that stretch and break into droplets by capillary action. At low Oh , lobe stretching and thinning is hindered by high surface tension and splitting of the original Kelvin-Helmholtz vortex, preventing early hole formation. Corrugations form on the lobe edges, influenced by the split vortices, and stretch to form ligaments. Both mechanisms are present in a transition region that shifts in Oh values based on the liquid/gas density ratio. Different characteristic times exist for the hole formation and the lobe and ligament stretching, related to surface tension and liquid viscosity, respectively. In the transition region, both times are of the same order. Streamwise vorticity triggers the 3D instabilities. Vorticity stretching and baroclinicity dominate, while the spanwise and cross-flow vorticity tilting are less important early in the breakup.

  9. Modulation of leading edge vorticity and aerodynamic forces in flexible flapping wings. (United States)

    Zhao, Liang; Deng, Xinyan; Sane, Sanjay P


    In diverse biological flight systems, the leading edge vortex has been implicated as a flow feature of key importance in the generation of flight forces. Unlike fixed wings, flapping wings can translate at higher angles of attack without stalling because their leading edge vorticity is more stable than the corresponding fixed wing case. Hence, the leading edge vorticity has often been suggested as the primary determinant of the high forces generated by flapping wings. To test this hypothesis, it is necessary to modulate the size and strength of the leading edge vorticity independently of the gross kinematics while simultaneously monitoring the forces generated by the wing. In a recent study, we observed that forces generated by wings with flexible trailing margins showed a direct dependence on the flexural stiffness of the wing. Based on that study, we hypothesized that trailing edge flexion directly influences leading edge vorticity, and thereby the magnitude of aerodynamic forces on the flexible flapping wings. To test this hypothesis, we visualized the flows on wings of varying flexural stiffness using a custom 2D digital particle image velocimetry system, while simultaneously monitoring the magnitude of the aerodynamic forces. Our data show that as flexion decreases, the magnitude of the leading edge vorticity increases and enhances aerodynamic forces, thus confirming that the leading edge vortex is indeed a key feature for aerodynamic force generation in flapping flight. The data shown here thus support the hypothesis that camber influences instantaneous aerodynamic forces through modulation of the leading edge vorticity.

  10. Pinning, flux diodes and ratchets for vortices interacting with conformal pinning arrays (United States)

    Olson Reichhardt, C. J.; Wang, Y. L.; Xiao, Z. L.; Kwok, W. K.; Ray, D.; Reichhardt, C.; Jankó, B.


    A conformal pinning array can be created by conformally transforming a uniform triangular pinning lattice to produce a new structure in which the six-fold ordering of the original lattice is conserved but where there is a spatial gradient in the density of pinning sites. Here we examine several aspects of vortices interacting with conformal pinning arrays and how they can be used to create a flux flow diode effect for driving vortices in different directions across the arrays. Under the application of an ac drive, a pronounced vortex ratchet effect occurs where the vortices flow in the easy direction of the array asymmetry. When the ac drive is applied perpendicular to the asymmetry direction of the array, it is possible to realize a transverse vortex ratchet effect where there is a generation of a dc flow of vortices perpendicular to the ac drive due to the creation of a noise correlation ratchet by the plastic motion of the vortices. We also examine vortex transport in experiments and compare the pinning effectiveness of conformal arrays to uniform triangular pinning arrays. We find that a triangular array generally pins the vortices more effectively at the first matching field and below, while the conformal array is more effective at higher fields where interstitial vortex flow occurs.

  11. Nonlinear axisymmetric and three-dimensional vorticity dynamics in a swirling jet model (United States)

    Martin, J. E.; Meiburg, E.


    The mechanisms of vorticity concentration, reorientation, and stretching are investigated in a simplified swirling jet model, consisting of a line vortex along the jet axis surrounded by a jet shear layer with both azimuthal and streamwise vorticity. Inviscid three-dimensional vortex dynamics simulations demonstrate the nonlinear interaction and competition between a centrifugal instability and Kelvin-Helmholtz instabilities feeding on both components of the base flow vorticity. Under axisymmetric flow conditions, it is found that the swirl leads to the emergence of counterrotating vortex rings, whose circulation, in the absence of viscosity, can grow without bounds. Scaling laws are provided for the growth of these rings, which trigger a pinch-off mechanism resulting in a strong decrease of the local jet diameter. In the presence of an azimuthal disturbance, the nonlinear evolution of the flow depends strongly on the initial ratio of the azimuthal and axisymmetric perturbation amplitudes. The long term dynamics of the jet can be dominated by counterrotating vortex rings connected by braid vortices, by like-signed rings and streamwise braid vortices, or by wavy streamwise vortices alone.

  12. On the odd and even secondary instabilities of Görtler vortices (United States)

    Souza, Leandro F.


    Boundary layer flows over concave wall can be unstable to disturbances giving rise to streamwise counter-rotating vortices known as Görtler vortices. These vortices in its nonlinear form are responsible for a strong distortion of the streamwise velocity profiles in the wall-normal and spanwise directions. The resulting inflectional velocity profiles are unstable to unsteady disturbances. These disturbances are called secondary instabilities and can develop into horseshoe vortices or a sinuous motion of the Görtler vortices. These types of secondary instabilities are known as even (varicose) and odd (sinuous) modes, respectively. Although many studies focused this subject, it has not been stated which mode dominates the transition process. In the present study the secondary instability of Görtler flow is investigated using high-order spatial numerical simulation. Multi-frequency unsteady disturbances are introduced with the same spanwise wavelength as the Görtler vortices, but different spanwise phases. Three different spanwise phases are used and the effect on the secondary instability is analyzed. Both, even and odd secondary instabilities are observed, according to the relative spanwise position of the unsteady disturbances. The growth analysis for each secondary crossplane instability mode is made using a temporal Fourier analysis and the physics is explored with the aid of the flow structures visualization. The results introducing disturbances that give rise to odd and even modes simultaneously show that, for the spanwise wavelength analyzed, the odd modes grow first and dominate the transition process.

  13. Flow-pattern analysis in open and closed square ducts: A comparative investigation of corner vortices (United States)

    Kriegseis, Jochen; Vaas, Markus; Frohnapfel, Bettina


    In the present study secondary flows in straight square ducts are investigated experimentally for open and closed geometries. The flow of the closed square duct typically consists of a set of eight equal-sized counter-rotating vortices. In contrast, the flow in an open flume of (identical) square geometry is considered, where the development of secondary vortices is strongly affected by the presence of the free surface. Stereo Particle Image Velocimetry (SPIV) experiments have been performed so as to measure the flow in both geometries for varying Reynolds numbers. From the resulting 2D3C velocity information secondary vortices (superimposed onto the mean primary flow) are identified. As expected, the upper bisectors' flow topology differs significantly between open and closed ducts, where typical inner and outer vortices are identified from the open-duct data. Interestingly, the secondary vortices of the lower bisectors of both duct flows reveal a similar topology. In order to study this seeming similarit more rigorously, a modal analysis of the respective flow data is performed by means of proper orthogonal decomposition (POD). As such, similarities and limitations of the comparability of the respective corner vortices are uncovered and discussed as function of Reynolds number. J. Kriegseis acknowledges financial support by the German Academic Exchange Service (DAAD)

  14. Extended Mixed Vector Equilibrium Problems

    Directory of Open Access Journals (Sweden)

    Mijanur Rahaman


    Full Text Available We study extended mixed vector equilibrium problems, namely, extended weak mixed vector equilibrium problem and extended strong mixed vector equilibrium problem in Hausdorff topological vector spaces. Using generalized KKM-Fan theorem (Ben-El-Mechaiekh et al.; 2005, some existence results for both problems are proved in noncompact domain.

  15. Vector-borne diseases: the basic reproduction number R0 and risk maps

    NARCIS (Netherlands)

    Hartemink, N.A.


    This thesis deals with the derivation of the basic reproduction number (R0) for vector-borne diseases, in the context of studying the effect of climate change on the risk of emergence diseases. Vector-borne diseases are transmitted from an infected individual to another individual by vectors,

  16. Diffractive S and D-wave vector mesons in deep inelastic scattering


    Ivanov, I. P.; Nikolaev, N.N.


    We derive helicity amplitudes for diffractive leptoproduction of the S and D wave states of vector mesons. We predict a dramatically different spin dependence for production of the S and D wave vector mesons. We find very small $R=\\sigma_{L}/\\sigma_{T}$ and abnormally large higher twist effects in production of longitudinally polarized D-wave vector mesons.

  17. Construction of a novel lentiviral vector carrying human B-domain ...

    African Journals Online (AJOL)

    Vectors derived from human immunodeficiency virus-1 (HIV-1) are highly efficient vehicles for gene delivery. The present study aimed to establish a potent expression system of human factor VIII (FVIII) with lentiviral vectors. FVIII³BD gene was obtained by enzyme digestion and inserted into lentiviral vector pXZ208 driven by ...

  18. Upwelling Response to Hurricane Isaac in Geostrophic Oceanic Vortices (United States)

    Jaimes, B.; Shay, L. K.; Brewster, J. K.; Schuster, R.


    As a tropical cyclone (TC) moves over the ocean, the cyclonic curl of the wind stress produces a region of upwelling waters under the TC center that is compensated by downwelling waters at regions outside the center. Direct measurements conducted during hurricane Rita and recent numerical studies indicate that this is not necessarily the case when TCs move over geostrophic oceanic features, where its background relative vorticity impacts wind-driven horizontal current divergence and the upwelling velocity. Modulation of the upwelling response in these energetic oceanic regimes impacts vertical mixing across the oceanic mixed layer base, air-sea fluxes into the atmosphere, and ultimately storm intensity. As part of NOAA Intensity Forecasting Experiment, an experiment was conducted during the passage of TC Isaac over the energetic geostrophic eddy field in the Gulf of Mexico in August 2012. Expendable bathythermographs, current profilers, and conductivity-temperature-depth probes were deployed in Isaac from NOAA WP-3D aircraft during four in-storm flights to measure oceanic variability and its impact on TC-driven upwelling and surface fluxes of heat and momentum. During intensification to hurricane, the cyclonic curl of the wind stress of Isaac extended over a region of more than 300 km in diameter (4 to 5 times the radius of maximum winds). Isaac's center moved over a cold cyclonic feature, while its right and left sides moved over warm anticyclones. Contrasting upwelling and downwelling regimes developed inside the region of cyclonic curl of the wind stress. Both positive (upwelling) and negative (downwelling) vertical displacements of 40 and 60 m, respectively, were measured inside the region of cyclonic curl of the wind stress, which are between 3 to 4 times larger than predicted vertical displacements for a quiescent ocean based on scaling arguments. Oceanic mixed layer (OML) currents of 0.2 to 0.7 m s-1 were measured, which are about 50% smaller than the

  19. Generation of cylindrically polarized vector vortex beams with digital micromirror device

    Energy Technology Data Exchange (ETDEWEB)

    Gong, Lei; Liu, Weiwei; Wang, Meng; Zhong, Mincheng; Wang, Ziqiang; Li, Yinmei, E-mail: [Department of Optics and Optical Engineering, University of Science and Technology of China, Hefei, Anhui Province 230026 (China); Ren, Yuxuan [National Center for Protein Sciences Shanghai, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, CAS, Shanghai 201210 (China)


    We propose a novel technique to directly transform a linearly polarized Gaussian beam into vector-vortex beams with various spatial patterns. Full high-quality control of amplitude and phase is implemented via a Digital Micro-mirror Device (DMD) binary holography for generating Laguerre-Gaussian, Bessel-Gaussian, and helical Mathieu–Gaussian modes, while a radial polarization converter (S-waveplate) is employed to effectively convert the optical vortices into cylindrically polarized vortex beams. Additionally, the generated vector-vortex beams maintain their polarization symmetry after arbitrary polarization manipulation. Due to the high frame rates of DMD, rapid switching among a series of vector modes carrying different orbital angular momenta paves the way for optical microscopy, trapping, and communication.

  20. Downstream evolution of unconfined vortices: mechanical and thermal aspects (United States)

    Pérez-Saborid, M.; Herrada, M. A.; Gómez-Barea, A.; Barrero, A.


    We present a numerical study of the downstream evolution (mechanical and thermal) of vortex-jet cores whose velocity and temperature fields far from the axis match a family of inviscid and non-conducting vortices. The far-velocity field is rotational, except for a particular case which corresponds to the well-known Long's vortex. The evolution of the vortex core depends on both the conditions at a certain upstream station, characterized by the dimensionless value of the velocity at the axis, and a dimensionless swirling parameter L defined as the ratio of the values of the azimuthal and axial velocities outside the vortex core. This numerical study, based on the quasi-cylindrical approximation (QC) of the Navier Stokes equations, determines the conditions under which the vortex evolution proceeds smoothly, eventually reaching an asymptotic self-similar behaviour as described in the literature (Fernández-Feria, Fernández de la Mora & Barrero 1995; Herrada, Pérez-Saborid & Barrero 1999), or breaks in a non-slender solution (vortex breakdown). In particular, the critical value L = Lb(a) beyond which vortex breakdown occurs downstream is a function of a dimensionless parameter a characterizing the axial momentum of the vortex jet at an initial upstream station. It is found numerically that for very large values of a this vortex breakdown criterion tends to an asymptote which is precisely the value L = L* predicted by the self-similar analysis, and beyond which a self-similar structure of the vortex core does not exist. In addition, the computation of the total temperature field provides useful information on the physical mechanisms responsible for the thermal separation phenomenon observed in Ranque Hilsch tubes and other swirling jet devices. In particular, the mechanical work of viscous forces which gives rise to an intense loss of kinetic energy during the initial stages of the evolution has been identified as the physical mechanism responsible for thermal

  1. Thermal Separation in a Family of Self-Similar Vortices (United States)

    Herrada, M. A.; Perez-Saborid, M.; Barrero, A.


    Swirling flows have a very wide range of applications and exhibit a variety of interesting features. One of them is the phenomenon of thermal separation, the so-called Ranque-Hilsch effect, observed under certain conditions in swirling gas jets. The cold gas stream is confined near the jet axis while the hotter one locates at the periphery. The low efficiency of the existing Ranque-Hilsch tubes is probably due to an incomplete understanding of the basic fluid mechanics involved in the thermal separation effect. To gain insight into the phenomenon under consideration, we have analyzed the near axis boundary layer of a gas jet driven by a class of self-similar inviscid vortical incompressible flow which satisfies the Euler equations and whose velocity field, in cylindrical coordinates, behaves near the axis as v = Wo r^m-2(e_z+L e_θ), where Wo and L are arbitrary positive constants and m is any real number such as 1<= m<2. In our analysis, we have considered low Mach number flows with small relative variations in the gas temperature, so that the mechanical and thermal problems can be solved separately. For a given value of m, the difference between the maximum and minimum stagnation temperature of the flow (thermal separation) can be plotted as a function of the Squire parameter L which is directly related to the swirl strength. We found that there is an optimum value L_op for which thermal separation reaches a sharp maximum so that small differences in the value of L-L_op drastically reduces the thermal separation.

  2. Turbulence and vorticity in Galaxy clusters generated by structure formation (United States)

    Vazza, F.; Jones, T. W.; Brüggen, M.; Brunetti, G.; Gheller, C.; Porter, D.; Ryu, D.


    Turbulence is a key ingredient for the evolution of the intracluster medium, whose properties can be predicted with high-resolution numerical simulations. We present initial results on the generation of solenoidal and compressive turbulence in the intracluster medium during the formation of a small-size cluster using highly resolved, non-radiative cosmological simulations, with a refined monitoring in time. In this first of a series of papers, we closely look at one simulated cluster whose formation was distinguished by a merger around z ˜ 0.3. We separate laminar gas motions, turbulence and shocks with dedicated filtering strategies and distinguish the solenoidal and compressive components of the gas flows using Hodge-Helmholtz decomposition. Solenoidal turbulence dominates the dissipation of turbulent motions (˜95 per cent) in the central cluster volume at all epochs. The dissipation via compressive modes is found to be more important (˜30 per cent of the total) only at large radii (≥0.5rvir) and close to merger events. We show that enstrophy (vorticity squared) is good proxy of solenoidal turbulence. All terms ruling the evolution of enstrophy (I.e. baroclinic, compressive, stretching and advective terms) are found to be significant, but in amounts that vary with time and location. Two important trends for the growth of enstrophy in our simulation are identified: first, enstrophy is continuously accreted into the cluster from the outside, and most of that accreted enstrophy is generated near the outer accretion shocks by baroclinic and compressive processes. Secondly, in the cluster interior vortex, stretching is dominant, although the other terms also contribute substantially.

  3. Dynamics of vortices and active matter on structured substrates (United States)

    Ray, Dipanjan

    This work investigates various physical problems under the general category of particles being driven over an ordered or disordered substrate. The general problem has clear applications to frictional phenomena, ranging from stick-slip motion to earthquakes; here we consider more novel scenarios taken from the fields of superconductivity and biophysics. The "conformal crystal" structure is investigated in the context of superconducting vortex pinning. This 2D structure is generated mathematically by a conformal transformation of a regular hexagonal lattice, and possesses local hexagonal ordering, but globally features a density gradient in one dimension and an arching structure in the other dimension. A vortex pinning array based on this structure is shown to have superior magnetization and transport properties as compared to other previously considered pinning arrays, and is used to construct a highly effective ratchet for vortices. An Archimedean pinning structure, with two characteristic length scales, is also considered, as an intermediate case between periodic pinning with a single length scale, and conformal pinning with a continuum of scales due to the density gradient. Magnetization studies reveal a variety of novel vortex states at integer and fractional matching fields, which are not present in either periodic or conformal pinning. Finally, an nanoassembly experiment is simulated where the particles affect the substrate, as opposed to the more common reverse scenario which underlies the other topics in this work. The energy of run-and-tumble active matter particles (such as E. coli bacteria undergoing chemotactic motion) is harnessed to push together two movable walls arranged in a Casimir geometry.

  4. Gaps and rings carved by vortices in protoplanetary dust (United States)

    Barge, Pierre; Ricci, Luca; Carilli, Christopher Luke; Previn-Ratnasingam, Rathish


    Context. Large-scale vortices in protoplanetary disks are thought to form and survive for long periods of time. Hence, they can significantly change the global disk evolution and particularly the distribution of the solid particles embedded in the gas, possibly explaining asymmetries and dust concentrations recently observed at submillimeter and millimeter wavelengths. Aims: We investigate the spatial distribution of dust grains using a simple model of protoplanetary disk hosted by a giant gaseous vortex. We explore the dependence of the results on grain size and deduce possible consequences and predictions for observations of the dust thermal emission at submillimeter and millimeter wavelengths. Methods: Global 2D simulations with a bi-fluid code are used to follow the evolution of a single population of solid particles aerodynamically coupled to the gas. Possible observational signatures of the dust thermal emission are obtained using simulators of ALMA and Nest Generation Very Large Array (ngVLA) observations. Results: We find that a giant vortex not only captures dust grains with Stokes number St< 1 but can also affect the distribution of larger grains (with St 1) carving a gap associated with a ring composed of incompletely trapped particles. The results are presented for different particle sizes and associated with their possible signatures in disk observations. Conclusions: Gap clearing in the dust spatial distribution could be due to the interaction with a giant gaseous vortex and their associated spiral waves without the gravitational assistance of a planet. Hence, strong dust concentrations at short sub-mm wavelengths associated with a gap and an irregular ring at longer mm and cm wavelengths could indicate the presence of an unseen gaseous vortex.

  5. APOBEC3-mediated hypermutation of retroviral vectors produced from some retrovirus packaging cell lines. (United States)

    Miller, A D; Metzger, M J


    APOBEC3 proteins are packaged into retrovirus virions and can hypermutate retroviruses during reverse transcription. We found that HT-1080 human fibrosarcoma cells hypermutate retroviruses, and that the HT-1080 cell-derived FLYA13 retrovirus packaging cells also hypermutate a retrovirus vector produced using these cells. We found no hypermutation of the same vector produced by the mouse cell-derived packaging line PT67 or by human 293 cells transfected with the vector and retrovirus packaging plasmids. We expect that avoidance of vector hypermutation will be particularly important for vectors used in gene therapy, wherein mutant proteins might stimulate deleterious immune responses.

  6. Formation and characterization of the vortices generated by a DBD plasma actuator in burst mode (United States)

    Mishra, Bal Krishan; Panigrahi, P. K.


    The present study reports the formation and evolution characteristics of the continuously generated vortical structure and resulting flow field in quiescent air induced by a dielectric-barrier-discharge (DBD) plasma actuator in burst mode operation. A starting vortex is formed during the initial actuation period, which disappears after a small time interval for continuous mode operation of the DBD plasma actuator. A burst input signal to the actuator generates a train of self-similar vortices. The behaviour of vortices and the average flow field induced by the actuator has been studied using high speed schlieren visualization and particle image velocimetry technique for different actuation amplitude and duty cycle parameters. These repeating vortices travel faster than the starting vortex, and the vortex core velocity of these repeating vortices increases with increase in duty cycle parameter. Fuller u-velocity profile, higher v-velocity near the edge of the outer shear layer region, and higher growth of the wall jet thickness is observed due to enhanced entrainment by repeating vortices for burst mode operation. The repeating vortices travel at an angle of 21° relative to the wall surface for duty cycle parameter of 90.9% in comparison to 31° for the starting vortex. Self-similarity of the velocity profile is delayed in the streamwise direction for burst mode operation in comparison to that for the continuous mode of operation. This can be attributed to delay in attaining the maximum velocity of the wall jet profile and presence of coherent structures for the burst mode operation. The non-dimensional vortex core location and size for repeating vortices follow power law fit similar to the starting vortex with difference in value of the power law exponent. The phase difference between the input voltage and current drawn is in the range of π/12 to π/9 (in radians) for both continuous and burst mode operation indicating identical electrical behaviour of the

  7. Bunyavirus-Vector Interactions

    Directory of Open Access Journals (Sweden)

    Kate McElroy Horne


    Full Text Available The Bunyaviridae family is comprised of more than 350 viruses, of which many within the Hantavirus, Orthobunyavirus, Nairovirus, Tospovirus, and Phlebovirus genera are significant human or agricultural pathogens. The viruses within the Orthobunyavirus, Nairovirus, and Phlebovirus genera are transmitted by hematophagous arthropods, such as mosquitoes, midges, flies, and ticks, and their associated arthropods not only serve as vectors but also as virus reservoirs in many cases. This review presents an overview of several important emerging or re-emerging bunyaviruses and describes what is known about bunyavirus-vector interactions based on epidemiological, ultrastructural, and genetic studies of members of this virus family.

  8. Free topological vector spaces


    Gabriyelyan, Saak S.; Morris, Sidney A.


    We define and study the free topological vector space $\\mathbb{V}(X)$ over a Tychonoff space $X$. We prove that $\\mathbb{V}(X)$ is a $k_\\omega$-space if and only if $X$ is a $k_\\omega$-space. If $X$ is infinite, then $\\mathbb{V}(X)$ contains a closed vector subspace which is topologically isomorphic to $\\mathbb{V}(\\mathbb{N})$. It is proved that if $X$ is a $k$-space, then $\\mathbb{V}(X)$ is locally convex if and only if $X$ is discrete and countable. If $X$ is a metrizable space it is shown ...

  9. Matrix vector analysis

    CERN Document Server

    Eisenman, Richard L


    This outstanding text and reference applies matrix ideas to vector methods, using physical ideas to illustrate and motivate mathematical concepts but employing a mathematical continuity of development rather than a physical approach. The author, who taught at the U.S. Air Force Academy, dispenses with the artificial barrier between vectors and matrices--and more generally, between pure and applied mathematics.Motivated examples introduce each idea, with interpretations of physical, algebraic, and geometric contexts, in addition to generalizations to theorems that reflect the essential structur

  10. Scalar-vector bootstrap

    Energy Technology Data Exchange (ETDEWEB)

    Rejon-Barrera, Fernando [Institute for Theoretical Physics, University of Amsterdam,Science Park 904, Postbus 94485, 1090 GL, Amsterdam (Netherlands); Robbins, Daniel [Department of Physics, Texas A& M University,TAMU 4242, College Station, TX 77843 (United States)


    We work out all of the details required for implementation of the conformal bootstrap program applied to the four-point function of two scalars and two vectors in an abstract conformal field theory in arbitrary dimension. This includes a review of which tensor structures make appearances, a construction of the projectors onto the required mixed symmetry representations, and a computation of the conformal blocks for all possible operators which can be exchanged. These blocks are presented as differential operators acting upon the previously known scalar conformal blocks. Finally, we set up the bootstrap equations which implement crossing symmetry. Special attention is given to the case of conserved vectors, where several simplifications occur.

  11. Multithreading in vector processors

    Energy Technology Data Exchange (ETDEWEB)

    Evangelinos, Constantinos; Kim, Changhoan; Nair, Ravi


    In one embodiment, a system includes a processor having a vector processing mode and a multithreading mode. The processor is configured to operate on one thread per cycle in the multithreading mode. The processor includes a program counter register having a plurality of program counters, and the program counter register is vectorized. Each program counter in the program counter register represents a distinct corresponding thread of a plurality of threads. The processor is configured to execute the plurality of threads by activating the plurality of program counters in a round robin cycle.

  12. Two-host, two-vector basic reproduction ratio (R(0 for bluetongue.

    Directory of Open Access Journals (Sweden)

    Joanne Turner

    Full Text Available Mathematical formulations for the basic reproduction ratio (R(0 exist for several vector-borne diseases. Generally, these are based on models of one-host, one-vector systems or two-host, one-vector systems. For many vector borne diseases, however, two or more vector species often co-occur and, therefore, there is a need for more complex formulations. Here we derive a two-host, two-vector formulation for the R(0 of bluetongue, a vector-borne infection of ruminants that can have serious economic consequences; since 1998 for example, it has led to the deaths of well over 1 million sheep in Europe alone. We illustrate our results by considering the situation in South Africa, where there are two major hosts (sheep, cattle and two vector species with differing ecologies and competencies as vectors, for which good data exist. We investigate the effects on R(0 of differences in vector abundance, vector competence and vector host preference between vector species. Our results indicate that R(0 can be underestimated if we assume that there is only one vector transmitting the infection (when there are in fact two or more and/or vector host preferences are overlooked (unless the preferred host is less beneficial or more abundant. The two-host, one-vector formula provides a good approximation when the level of cross-infection between vector species is very small. As this approaches the level of intraspecies infection, a combination of the two-host, one-vector R(0 for each vector species becomes a better estimate. Otherwise, particularly when the level of cross-infection is high, the two-host, two-vector formula is required for accurate estimation of R(0. Our results are equally relevant to Europe, where at least two vector species, which co-occur in parts of the south, have been implicated in the recent epizootic of bluetongue.

  13. Generalized vector calculus on convex domain (United States)

    Agrawal, Om P.; Xu, Yufeng


    In this paper, we apply recently proposed generalized integral and differential operators to develop generalized vector calculus and generalized variational calculus for problems defined over a convex domain. In particular, we present some generalization of Green's and Gauss divergence theorems involving some new operators, and apply these theorems to generalized variational calculus. For fractional power kernels, the formulation leads to fractional vector calculus and fractional variational calculus for problems defined over a convex domain. In special cases, when certain parameters take integer values, we obtain formulations for integer order problems. Two examples are presented to demonstrate applications of the generalized variational calculus which utilize the generalized vector calculus developed in the paper. The first example leads to a generalized partial differential equation and the second example leads to a generalized eigenvalue problem, both in two dimensional convex domains. We solve the generalized partial differential equation by using polynomial approximation. A special case of the second example is a generalized isoperimetric problem. We find an approximate solution to this problem. Many physical problems containing integer order integrals and derivatives are defined over arbitrary domains. We speculate that future problems containing fractional and generalized integrals and derivatives in fractional mechanics will be defined over arbitrary domains, and therefore, a general variational calculus incorporating a general vector calculus will be needed for these problems. This research is our first attempt in that direction.

  14. Effects of wing locations on wing rock induced by forebody vortices

    Directory of Open Access Journals (Sweden)

    Ma Baofeng


    Full Text Available Previous studies have shown that asymmetric vortex wakes over slender bodies exhibit a multi-vortex structure with an alternate arrangement along a body axis at high angle of attack. In this investigation, the effects of wing locations along a body axis on wing rock induced by forebody vortices was studied experimentally at a subcritical Reynolds number based on a body diameter. An artificial perturbation was added onto the nose tip to fix the orientations of forebody vortices. Particle image velocimetry was used to identify flow patterns of forebody vortices in static situations, and time histories of wing rock were obtained using a free-to-roll rig. The results show that the wing locations can affect significantly the motion patterns of wing rock owing to the variation of multi-vortex patterns of forebody vortices. As the wing locations make the forebody vortices a two-vortex pattern, the wing body exhibits regularly divergence and fixed-point motion with azimuthal variations of the tip perturbation. If a three-vortex pattern exists over the wing, however, the wing-rock patterns depend on the impact of the highest vortex and newborn vortex. As the three vortices together influence the wing flow, wing-rock patterns exhibit regularly fixed-points and limit-cycled oscillations. With the wing moving backwards, the newborn vortex becomes stronger, and wing-rock patterns become fixed-points, chaotic oscillations, and limit-cycled oscillations. With further backward movement of wings, the vortices are far away from the upper surface of wings, and the motions exhibit divergence, limit-cycled oscillations and fixed-points. For the rearmost location of the wing, the wing body exhibits stochastic oscillations and fixed-points.

  15. Production and titration of lentiviral vectors. (United States)

    Barde, Isabelle; Salmon, Patrick; Trono, Didier


    Lentiviral vectors have emerged over the last decade as powerful, reliable, and safe tools for stable gene transfer in a wide variety of mammalian cells. Unlike other vectors derived from oncoretroviruses, they allow for stable gene delivery into most nondividing primary cells, including neurons. This is why lentivectors (LVs) are becoming the most useful and promising tools in the field of neuroscience, not only for research, but also for future gene and cell therapy approaches. LVs derived from HIV-1 have gradually evolved to display many desirable features aimed at increasing both their safety and their versatility. These latest designs are reviewed in this unit. This unit also describes protocols for production and titration of LVs that can be implemented in a research laboratory setting, with an emphasis on standardization to improve transposability of results between laboratories.

  16. Support Vector Components Analysis

    NARCIS (Netherlands)

    van der Ree, Michiel; Roerdink, Johannes; Phillips, Christophe; Garraux, Gaetan; Salmon, Eric; Wiering, Marco


    In this paper we propose a novel method for learning a distance metric in the process of training Support Vector Machines (SVMs) with the radial basis function kernel. A transformation matrix is adapted in such a way that the SVM dual objective of a classification problem is optimized. By using a

  17. Sesquilinear uniform vector integral

    Indian Academy of Sciences (India)

    1Faculty of Mathematics and Computer Science, University of Bucharest, Bucharest,. Academiei Str., 14, 010014, Romania. 2Technical University of Civil ... an integral of scalar functions with respect to vector measures, Dunford and his school introduced the spectral operators, thus founding the present operator theory (see ...

  18. Orthogonalisation of Vectors

    Indian Academy of Sciences (India)

    Home; Journals; Resonance – Journal of Science Education; Volume 5; Issue 3. Orthogonalisation of Vectors - Matrix Decomposition and Approximation Problems. Rajendra Bhatia. General Article Volume 5 ... Author Affiliations. Rajendra Bhatia1. Indian Statistical Institute 7, SJS Sansanwal Marg, New Delhi 110 016, India.

  19. Vector-borne Infections

    Centers for Disease Control (CDC) Podcasts


    This podcast discusses emerging vector-borne pathogens, their role as prominent contributors to emerging infectious diseases, how they're spread, and the ineffectiveness of mosquito control methods.  Created: 4/18/2011 by National Center for Emerging Zoonotic and Infectious Diseases (NCEZID).   Date Released: 4/27/2011.

  20. Rotational accelerations stabilize leading edge vortices on revolving fly wings. (United States)

    Lentink, David; Dickinson, Michael H


    should therefore be able to generate a stable LEV, a prediction that is supported by recent findings for several insects, one bat, one bird and one fish. This suggests that force augmentation through stably attached (leading edge) vortices could represent a convergent solution for the generation of high fluid forces over a quite large range in size.