WorldWideScience

Sample records for volumes 1-6 recount

  1. Cesium-137, a drama recounted; Cesio-137, um drama recontado

    Energy Technology Data Exchange (ETDEWEB)

    Vieira, Suzane de Alencar

    2013-01-15

    The radiological accident with Cesium-137, which started on Goiania in 1987, did not stop with the end of radiological contamination and continues in a judicial, scientific and narrative process of identification and recognition of new victims. The drama occupies a central place on the dynamics of radiological event, as it extends its limits, inflects its intensity and updates the event. As a narrative of the event, the ethnography incorporates and brings up to date the drama as an analysis landmark and the description of the theme as it is absorbed by a dramatic process. Cesium-137, a drama recounted is a textual experimentation based on real events and characters picked out from statements reported in various narratives about the radiological accident. (author)

  2. Developing Environment–Based Materials to Teach Writing in Recount Texts

    Directory of Open Access Journals (Sweden)

    Fauziah Ratna Hapsari

    2015-10-01

    Full Text Available The tenth graders of State Senior High School 2 Semarang had problems to write their experiences. Unfortunately the existing materials were not appropriate to facilitate the learners to write recount texts. Hence, this study would like to develop environment – based materials to teach writing recount texts. It was inspired by the previous studies held by Gürsoy (2010 and Hauschild (2012. The materials were expected to improve learners’ writing competence of recount texts by applying topics which learners found in their daily lives, that is, environmental education. This study employed Research and Development design adapted from Borg and Gall (2003. The study found that applying the environment – based materials to teach writing recount texts gained positive effects. Moreover, the test of effectiveness reported significant improvement. The average score of pre-test was 48.94 and of post-test was 81.61. Therefore, English teachers are suggested to employ the materials. It is also necessary to conduct further studies to gain more positive effects toward teaching and learning process and to improve the learners’ writing competence. Keywords: Teaching writing; recount text; environment – based materials; contextual teaching and learningCopyright © 2015 by Al-Ta'lim All right reserved

  3. Developing Environment–Based Materials to Teach Writing in Recount Texts

    Directory of Open Access Journals (Sweden)

    Fauziah Ratna Hapsari

    2015-10-01

    Full Text Available The tenth graders of State Senior High School 2 Semarang had problems to write their experiences. Unfortunately the existing materials were not appropriate to facilitate the learners to write recount texts. Hence, this study would like to develop environment – based materials to teach writing recount texts. It was inspired by the previous studies held by Gürsoy (2010 and Hauschild (2012. The materials were expected to improve learners’ writing competence of recount texts by applying topics which learners found in their daily lives, that is, environmental education. This study employed Research and Development design adapted from Borg and Gall (2003. The study found that applying the environment – based materials to teach writing recount texts gained positive effects. Moreover, the test of effectiveness reported significant improvement. The average score of pre-test was 48.94 and of post-test was 81.61. Therefore, English teachers are suggested to employ the materials. It is also necessary to conduct further studies to gain more positive effects toward teaching and learning process and to improve the learners’ writing competence. Keywords: Teaching writing; recount text; environment – based materials; contextual teaching and learningCopyright © 2015 by Al-Ta'lim All right reserved

  4. Draft environmental impact statement for the siting, construction, and operation of New Production Reactor capacity. Volume 2, Sections 1-6

    Energy Technology Data Exchange (ETDEWEB)

    1991-04-01

    This (EIS) assesses the potential environmental impacts, both on a broad programmatic level and on a project-specific level, concerning a proposed action to provide new tritium production capacity to meet the nation`s nuclear defense requirements well into the 21st century. A capacity equivalent to that of about a 3,000-megawatt (thermal) heavy-water reactor was assumed as a reference basis for analysis in this EIS; this is the approximate capacity of the existing production reactors at DOE`s Savannah River Site. The EIS programmatic alternatives address Departmental decisions to be made on whether to build new production facilities, whether to build one or more complexes, what size production capacity to provide, and when to provide this capacity. Project-specific impacts for siting, constructing, and operating new production reactor capacity are assessed for three alternative sites: the Hanford Site near Richland, Washington; the Idaho National Engineering Laboratory near Idaho Falls, Idaho; and the Savannah River Site. For each site, the impacts of three reactor technologies (and supporting facilities) are assessed: a heavy-water reactor, a light-water reactor, and a modular high-temperature gas-cooled reactor. Impacts of the no-action alternative also are assessed. The EIS evaluates impacts related to air quality; noise levels; surface water, groundwater, and wetlands; land use; recreation; visual environment; biotic resources; historical, archaeological, and cultural resources; socioeconomics; transportation; waste management; and human health and safety. The EIS describes in detail the potential radioactive releases from new production reactors and support facilities and assesses the potential doses to workers and the general public. This volume contains the analysis of programmatic alternatives, project alternatives, affected environment of alternative sites, environmental consequences, and environmental regulations and permit requirements.

  5. Science to support adaptive habitat management: Overton Bottoms North Unit, Big Muddy National Fish and Wildlife Refuge, Missouri [Volumes 1-6

    Science.gov (United States)

    Jacobson, Robert B.

    2006-01-01

    Extensive efforts are underway along the Lower Missouri River to rehabilitate ecosystem functions in the channel and flood plain. Considerable uncertainty inevitably accompanies ecosystem restoration efforts, indicating the benefits of an adaptive management approach in which management actions are treated as experiments, and results provide information to feed back into the management process. The Overton Bottoms North Unit of the Big Muddy National Fish and Wildlife Refuge is a part of the Missouri River Fish and Wildlife Habitat Mitigation Project. The dominant management action at the Overton Bottoms North Unit has been excavation of a side-channel chute to increase hydrologic connectivity and to enhance shallow, slow current-velocity habitat. The side-channel chute also promises to increase hydrologic gradients, and may serve to alter patterns of wetland inundation and vegetation community growth in undesired ways. The U.S. Geological Survey's Central Region Integrated Studies Program (CRISP) undertook interdisciplinary research at the Overton Bottoms North Unit in 2003 to address key areas of scientific uncertainty that were highly relevant to ongoing adaptive management of the site, and to the design of similar rehabilitation projects on the Lower Missouri River. This volume presents chapters documenting the surficial geologic, topographic, surface-water, and ground-water framework of the Overton Bottoms North Unit. Retrospective analysis of vegetation community trends over the last 10 years is used to evaluate vegetation responses to reconnection of the Overton Bottoms North Unit to the river channel. Quasi-experimental analysis of cottonwood growth rate variation along hydrologic gradients is used to evaluate sensitivity of terrestrial vegetation to development of aquatic habitats. The integrated, landscape-specific understanding derived from these studies illustrates the value of scientific information in design and management of rehabilitation projects.

  6. Persistent high fertility in Uganda: young people recount obstacles and enabling factors to use of contraceptives

    Directory of Open Access Journals (Sweden)

    Nalwadda Gorrette

    2010-09-01

    Full Text Available Abstract Background High fertility among young people aged 15-24 years is a public health concern in Uganda. Unwanted pregnancy, unsafe induced abortions and associated high morbidity and mortality among young women may be attributed to low contraceptive use. This study aims at exploring reasons for low contraceptive use among young people. Methods In 16 focus group discussions, the views of young people about obstacles and enabling factors to contraceptive use in Mityana and Mubende districts, Uganda were explored. The groups were homogeneously composed by married and unmarried men and women, between the ages of 15-24. The data obtained was analyzed using qualitative content analysis. Results Young men and women described multiple obstacles to contraceptive use. The obstacles were categorized as misconceptions and fears related to contraception, gender power relations, socio-cultural expectations and contradictions, short term planning, and health service barriers. Additionally, young people recounted several enabling factors that included female strategies to overcome obstacles, changing perceptions to contraceptive use, and changing attitude towards a small family size. Conclusions Our findings suggest changing perceptions and behavior shift towards contraceptive use and a small family size although obstacles still exist. Personalized strategies to young women and men are needed to motivate and assist young people plan their future families, adopt and sustain use of contraceptives. Reducing obstacles and reinforcing enabling factors through education, culturally sensitive behavior change strategies have the potential to enhance contraceptives use. Alternative models of contraceptive service delivery to young people are proposed.

  7. Grammatical Error Analysis in Recount Text Made by the Students of Cokroaminoto University of Palopo

    Directory of Open Access Journals (Sweden)

    Hermini Hermini

    2014-02-01

    Full Text Available This study aimed to find out (1 Grammatical errors in recount text made by the English Department students of the second and the sixth semester of Cokroaminoto University of Palopo, (2 the frequent grammatical errors made by the second and the sixth semester students of English department students (3 The difference of grammatical errors made by the second and the sixth semester students. The sample of the study was 723 sentences made by 30 students of the second semester and 30 students of the sixth semester students in academic year 2013/2014 that were taken by cluster random sampling technique. The sentences were 337 (46.61% simple sentences, 83(11.48% compound sentences, 218 (30.15% complex sentences, 85 (11.76% compound complex sentences. The data were collected by using two kinds of instruments namely: writing test to find the students’ grammatical errors and questionnaire to find the solution to prevent or minimize errors. Data on the students’ errors were analyzed by using descriptive statistics. The results of the study showed that the students made 832 errors classified into13 types of errors which consisted of 140 (16.82% errors in production of verb, 110 (13.22% errors in preposition, 106 (12,74% errors in distribution of verb, 98 (11.77% miscellaneous errors, 82 (9.85% errors in missing subject, 67(8.05% errors in part of speech, 61 (7,33% errors in irregular verbs, 58 (6.97% other errors in verb groups, 52(6.25% errors in the use of article, 24 (2.88% errors in gerund, 18 (2.16% errors in infinitive, 11(1.32% errors in pronoun/case, and 5 (0.6% errors in questions. The top six frequent grammatical errors made by the students were production of verb group, preposition, distribution of verb group, miscellaneous error, missing subject, and part of speech. The difference of both groups was the frequency in committing errors such as part of speech, irregular verb, infinitive verbs, and other errors in verb.

  8. 43 CFR 1.6 - Disciplinary proceedings.

    Science.gov (United States)

    2010-10-01

    ... 43 Public Lands: Interior 1 2010-10-01 2010-10-01 false Disciplinary proceedings. 1.6 Section 1.6 Public Lands: Interior Office of the Secretary of the Interior PRACTICES BEFORE THE DEPARTMENT OF THE INTERIOR § 1.6 Disciplinary proceedings. (a) Disciplinary proceedings may be instituted against anyone...

  9. Elementary Mathematics, Grades 1-6.

    Science.gov (United States)

    Nederland Independent School District, TX.

    GRADES OR AGES: Grades 1-6. SUBJECT MATTER: Elementary math. ORGANIZATION AND PHYSICAL APPEARANCE: Introductory material focuses on the philosophy and objectives of instructional material. The guide is divided into six units covering grades 1-6. Each unit presents the general goals, materials needed, minimum program, skills to be developed,…

  10. Volume and Surface-Enhanced Volume Negative Ion Sources

    CERN Document Server

    Stockli, M P

    2013-01-01

    H- volume sources and, especially, caesiated H- volume sources are important ion sources for generating high-intensity proton beams, which then in turn generate large quantities of other particles. This chapter discusses the physics and technology of the volume production and the caesium-enhanced (surface) production of H- ions. Starting with Bacal's discovery of the H- volume production, the chapter briefly recounts the development of some H- sources, which capitalized on this process to significantly increase the production of H- beams. Another significant increase was achieved in the 1990s by adding caesiated surfaces to supplement the volume-produced ions with surface-produced ions, as illustrated with other H- sources. Finally, the focus turns to some of the experience gained when such a source was successfully ramped up in H- output and in duty factor to support the generation of 1 MW proton beams for the Spallation Neutron Source.

  11. 14 CFR Sec. 1-6 - Accounting entities.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Accounting entities. Sec. 1-6 Section 1-6... REGULATIONS UNIFORM SYSTEM OF ACCOUNTS AND REPORTS FOR LARGE CERTIFICATED AIR CARRIERS General Accounting Provisions Sec. 1-6 Accounting entities. (a) Separate accounting records shall be maintained for each air...

  12. A Recounting of the Stages of French Communist Resistance during World War II and its Relationship to the French Resistance Overall

    Directory of Open Access Journals (Sweden)

    McManus, Eoin

    2015-06-01

    Full Text Available This paper will recount the stages of communist resistance in France during World War Two and its relationship to the French Resistance overall, as well as analyse the effect that communist resistance in France had on the war. It will outline the position of the French Communist Party (PCF in three different and distinctive periods: pre-1939, 1939 to the German invasion of the USSR in 1941, and from Operation Barbarossa to the liberation of France. This paper uses primary and secondary sources to create as balanced and objective an essay as possible. It will show that, for most of its existence, the PCF had been operating in a clandestine state and was thus the best prepared of any group for the outbreak of hostilities. The Communists were not necessarily late in joining the war, with resistance as we know it only beginning when the Communists began to resist. The relationship between the communist resisters and the other resistance groups is discussed, with particular reference to de Gaulle and shows the large degree of autonomy that the Communists maintained. What can be said with certainty of communist resistance in France is that it prevented greater casualties among the Allies through its acts of sabotage and its engagement of the enemy. This dissertation challenges the myth of the French Resistance and asserts that the Communists had the greatest influence of any group in the Resistance.

  13. Darwinian Controversies: An Historiographical Recounting

    Science.gov (United States)

    Depew, David J.

    2010-05-01

    This essay reviews key controversies in the history of the Darwinian research tradition: the Wilberforce-Huxley debate in 1860, early twentieth-century debates about the heritability of acquired characteristics and the consistency of Mendelian genetics with natural selection; the 1925 Scopes trial about teaching evolution; tensions about race, culture, and eugenics at the 1959 centenary celebration Darwin’s Origin of Species; adaptationism and its critics in the Sociobiology debate of 1970s and, more recently, Evolutionary Psychology; and current disputes about Intelligent Design. These controversies, I argue, are etched into public memory because they occur at the emotionally charged boundaries between public-political, technical-scientific, and personal-religious spheres of discourse. Over most of them falls the shadow of eugenics. The main lesson is that the history of Darwinism cannot be told except by showing the mutual influence of the different norms of discourse that obtain in the personal, technical, and public spheres. Nor can evolutionary biology successfully be taught to citizens and citizens-to-be until the fractious intersections between spheres of discourse have been made explicit. In the course of showing why, I take rival evolutionary approaches to be dynamical historical research traditions rather than static theories. Accordingly, I distinguish Darwin’s version of Darwinism from its later transformations. I pay special attention to the role Darwin assigned to development in evolution, which was marginalized by twentieth-century population genetical Darwinism, but has recently resurfaced in new forms. I also show how the disputed phrases “survival of the fittest” and “social Darwinism” have shaped personal anxieties about “Darwinism,” have provoked public opposition to teaching evolution in public schools, and have cast a shadow over efforts to effectively communicate to the public largely successful technical efforts to make evolutionary inquiry into a science.

  14. Darwinian Controversies: An Historiographical Recounting

    Science.gov (United States)

    Depew, David J.

    2010-01-01

    This essay reviews key controversies in the history of the Darwinian research tradition: the Wilberforce-Huxley debate in 1860, early twentieth-century debates about the heritability of acquired characteristics and the consistency of Mendelian genetics with natural selection; the 1925 Scopes trial about teaching evolution; tensions about race,…

  15. 49 CFR 172.525 - EXPLOSIVES 1.6 placard.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 2 2010-10-01 2010-10-01 false EXPLOSIVES 1.6 placard. 172.525 Section 172.525... SECURITY PLANS Placarding § 172.525 EXPLOSIVES 1.6 placard. (a) Except for size and color the EXPLOSIVES 1... subpart, the background color on the EXPLOSIVES 1.6 placard must be orange. The “*” shall be replaced...

  16. VT Lidar Slope (1.6 meter) - 2008 - West Franklin

    Data.gov (United States)

    Vermont Center for Geographic Information — (Link to Metadata) This metadata applies to the following collection area(s): Missisquoi Lower 2008 1.6m and related SLOPE datasets. Created using ArcGIS "SLOPE"...

  17. VT Lidar DSM (1.6 meter) - 2008 - West Franklin

    Data.gov (United States)

    Vermont Center for Geographic Information — (Link to Metadata) This metadata applies to the following collection area(s): Missisquoi Lower 2008 1.6m and related Digital Surface Model (DSM) data. This metadata...

  18. VT Lidar Slope (1.6 meter) - 2012 - Addison County

    Data.gov (United States)

    Vermont Center for Geographic Information — (Link to Metadata) This metadata applies to the following collection area(s): Addison County 2012 1.6m and related SLOPE datasets. Created using ArcGIS "SLOPE"...

  19. VT Lidar Aspect (1.6 meter) - 2012 - Addison County

    Data.gov (United States)

    Vermont Center for Geographic Information — (Link to Metadata) This metadata applies to the following collection area(s): Addison County 2012 1.6m and related ASPECT datasets. This metadata complies with the...

  20. VT Lidar Slope (1.6 meter) - 2010 - Missisquoi Upper

    Data.gov (United States)

    Vermont Center for Geographic Information — (Link to Metadata) This metadata applies to the following collection area(s): Missisquoi Upper 2010 1.6m and related SLOPE datasets. Created using ArcGIS "SLOPE"...

  1. VT Lidar Hillshade (1.6 meter) - 2008 - West Franklin

    Data.gov (United States)

    Vermont Center for Geographic Information — (Link to Metadata) This metadata applies to the following collection area(s): Missisquoi Lower 2008 1.6m and related "HILLSHADE" raster data. HILLSHADE data is for...

  2. VT Lidar DSM (1.6 meter) - 2012 - Addison

    Data.gov (United States)

    Vermont Center for Geographic Information — (Link to Metadata) This metadata applies to the following collection area(s): Addison County 2012 1.6m and related Digital Surface Model (DSM) data. This metadata...

  3. VT Lidar Hillshade (1.6 meter) - 2012 - Addison

    Data.gov (United States)

    Vermont Center for Geographic Information — (Link to Metadata) This metadata applies to the following collection area(s): Addison County 2012 1.6m and related "HILLSHADE" raster data. HILLSHADE data is for...

  4. Extraterrestrial processing and manufacturing of large space systems, volume 1, chapters 1-6

    Science.gov (United States)

    Miller, R. H.; Smith, D. B. S.

    1979-01-01

    Space program scenarios for production of large space structures from lunar materials are defined. The concept of the space manufacturing facility (SMF) is presented. The manufacturing processes and equipment for the SMF are defined and the conceptual layouts are described for the production of solar cells and arrays, structures and joints, conduits, waveguides, RF equipment radiators, wire cables, and converters. A 'reference' SMF was designed and its operation requirements are described.

  5. Diagnosis of bone and joint disorders. Second edition. Volumes 1-6

    Energy Technology Data Exchange (ETDEWEB)

    Resnick, D.; Niwayama, G.

    1988-01-01

    This book contains 98 chapters. Some of the chapter titles are: Plain Film Radiography: Routine and Specialized Techniques and Projections; Plain Film Radiography: Sources of Diagnostic Errors; Magnification Radiography, Xeroradiography; Magnetic Resonance Imaging; and Rheumatoid Arthritis and the Seronegative Spondyloarthropathies: Radiographic and Pathologic Concepts.

  6. Sixth Microgravity Fluid Physics and Transport Phenomena Conference: Exposition Topical Areas 1-6. Volume 2

    Science.gov (United States)

    Singh, Bhim (Compiler)

    2002-01-01

    The Sixth Microgravity Fluid Physics and Transport Phenomena Conference provides the scientific community the opportunity to view the current scope of the Microgravity Fluid Physics and Transport Phenomena Program, current research opportunities, and plans for the near future. The conference focuses not only on fundamental research but also on applications of this knowledge towards enabling future space exploration missions. A whole session dedicated to biological fluid physics shows increased emphasis that the program has placed on interdisciplinary research. The conference includes invited plenary talks, technical paper presentations, poster presentations, and exhibits. This CP (conference proceeding) is a compilation of the abstracts, presentations, and posters presented at the conference.

  7. Structure of Toxoplasma gondii fructose-1,6-bisphosphate aldolase

    Energy Technology Data Exchange (ETDEWEB)

    Boucher, Lauren E.; Bosch, Jürgen, E-mail: jbosch@jhu.edu [Johns Hopkins Bloomberg School of Public Health, 615 North Wolfe Street, Baltimore, MD 21205 (United States); Johns Hopkins Bloomberg School of Public Health, 615 North Wolfe Street, Baltimore, MD 21205 (United States)

    2014-08-29

    The structure of T. gondii fructose-1,6-bisphosphate aldolase, a glycolytic enzyme and structural component of the invasion machinery, was determined to a resolution of 2.0 Å. The apicomplexan parasite Toxoplasma gondii must invade host cells to continue its lifecycle. It invades different cell types using an actomyosin motor that is connected to extracellular adhesins via the bridging protein fructose-1,6-@@bisphosphate aldolase. During invasion, aldolase serves in the role of a structural bridging protein, as opposed to its normal enzymatic role in the glycolysis pathway. Crystal structures of the homologous Plasmodium falciparum fructose-1,6-bisphosphate aldolase have been described previously. Here, T. gondii fructose-1,6-bisphosphate aldolase has been crystallized in space group P22{sub 1}2{sub 1}, with the biologically relevant tetramer in the asymmetric unit, and the structure has been determined via molecular replacement to a resolution of 2.0 Å. An analysis of the quality of the model and of the differences between the four chains in the asymmetric unit and a comparison between the T. gondii and P. falciparum aldolase structures is presented.

  8. Coating experiment with 1.6-m vacuum evaporation chamber

    Science.gov (United States)

    Kamata, Yukiko; Hayashi, Saeko S.; Noguchi, Takeshi; Kanzawa, Tomio; Sasaki, Goro; Torii, Yasuo; Yutani, Masami; Ishikawa, Tsuyoshi

    1998-08-01

    We have conducted a series of coating experiments using the newly installed 1.6 m evaporation chamber at the Advanced Technology Center (ATC) of the National Astronomical Observatory of Japan. The main task of this chamber is to re-aluminize the 1.6 m mirror of the Infrared Simulator at the ATC. The design concept of the 1.6 m chamber is basically the same with the 8.3 m coating facility for Subaru Telescope. Therefore, we could utilize this chamber to evaluate the fundamental performance of the larger chamber. The extensive coating experiments were done in the spring, autumn of 1996, and autumn of 1997. Reduction of the number of the filaments has lead to the increase in their size, which caused difficulty in the annealing process. Attempts are focused on securing the sufficient metal loads on the filaments. Then the filaments are fired to measure the spray pattern of a single filament exposure, or the uniformity pattern resulted from the full setup of filament arrays. Using small slide glasses, the important parameters of the resultant reflecting film that are the thickness, the uniformity of the thickness, and the spectroscopic reflectance are measured. The absolute value of the reflectivity is estimated to be around 91% immediately after opening the chamber. In order to cover a wide range of observing wavelengths for the Infrared Simulator, and eventually for the optical-IR Subaru Telescope, it is necessary to seek after a higher evaporation rate with these chambers.

  9. Fructose-1,6-diphosphatase deficiency: a treatable neurometabolic disorder.

    Science.gov (United States)

    Kamate, Mahesh; Jambagi, Milind; Gowda, Prashanth; Sonoli, Smita

    2014-09-22

    Fructose-1,6-diphosphatase (FDPase) deficiency is usually considered an inborn error of fructose metabolism, however, strictly speaking it is a defect of gluconeogenesis. The disorder is manifested by the appearance of hypoglycaemia, ketosis and lactic acidosis (neonatally or later during fasting or induced by fructose) and may also be life-threatening. FDPase deficiency can be suspected using simple bedside tests such as glucometer random blood sugar, Benedict's test, Rothera's test and Seliwanoff's test. We report our experience with two cases of FDPase deficiency and review the relevant literature. We also describe the fructosuria in these cases during the crises period, which has not been stressed in the literature.

  10. CS1.6黄金体验

    Institute of Scientific and Technical Information of China (English)

    阿海

    2003-01-01

    之前推出的1.5版本经过了一个较稳定的阶段之后,《反思精英》(CS)制作小组Valve在今年将发布1.6正式版。随着CS1.6版测试版本的放出,我们终于体验到了“进化”的魅力,经过一段时间的测试、比较,现将新版本中值得注意的改动做一总结。

  11. All-printed diode operating at 1.6 GHz.

    Science.gov (United States)

    Sani, Negar; Robertsson, Mats; Cooper, Philip; Wang, Xin; Svensson, Magnus; Andersson Ersman, Peter; Norberg, Petronella; Nilsson, Marie; Nilsson, David; Liu, Xianjie; Hesselbom, Hjalmar; Akesso, Laurent; Fahlman, Mats; Crispin, Xavier; Engquist, Isak; Berggren, Magnus; Gustafsson, Göran

    2014-08-19

    Printed electronics are considered for wireless electronic tags and sensors within the future Internet-of-things (IoT) concept. As a consequence of the low charge carrier mobility of present printable organic and inorganic semiconductors, the operational frequency of printed rectifiers is not high enough to enable direct communication and powering between mobile phones and printed e-tags. Here, we report an all-printed diode operating up to 1.6 GHz. The device, based on two stacked layers of Si and NbSi2 particles, is manufactured on a flexible substrate at low temperature and in ambient atmosphere. The high charge carrier mobility of the Si microparticles allows device operation to occur in the charge injection-limited regime. The asymmetry of the oxide layers in the resulting device stack leads to rectification of tunneling current. Printed diodes were combined with antennas and electrochromic displays to form an all-printed e-tag. The harvested signal from a Global System for Mobile Communications mobile phone was used to update the display. Our findings demonstrate a new communication pathway for printed electronics within IoT applications.

  12. Efficient 1.6 Micron Laser Source for Methane DIAL

    Science.gov (United States)

    Shuman, Timothy; Burnham, Ralph; Nehrir, Amin R.; Ismail, Syed; Hair, Johnathan W.

    2013-01-01

    Methane is a potent greenhouse gas and on a per molecule basis has a warming influence 72 times that of carbon dioxide over a 20 year horizon. Therefore, it is important to look at near term radiative effects due to methane to develop mitigation strategies to counteract global warming trends via ground and airborne based measurements systems. These systems require the development of a time-resolved DIAL capability using a narrow-line laser source allowing observation of atmospheric methane on local, regional and global scales. In this work, a demonstrated and efficient nonlinear conversion scheme meeting the performance requirements of a deployable methane DIAL system is presented. By combining a single frequency 1064 nm pump source and a seeded KTP OPO more than 5 mJ of 1.6 µm pulse energy is generated with conversion efficiencies in excess of 20%. Even without active cavity control instrument limited linewidths (50 pm) were achieved with an estimated spectral purity of 95%. Tunable operation over 400 pm (limited by the tuning range of the seed laser) was also demonstrated. This source demonstrated the critical needs for a methane DIAL system motivating additional development of the technology.

  13. The impact of protocluster environments at z = 1.6

    CERN Document Server

    Hatch, N A; Muldrew, S I; Hartley, W G; Almaini, O; Conselice, C J; Simpson, C J

    2016-01-01

    We investigate the effects of dense environments on galaxy evolution by examining how the properties of galaxies in the z = 1.6 protocluster Cl 0218.3-0510 depend on their location. We determine galaxy properties using spectral energy distribution fitting to 14-band photometry, including data at three wavelengths that tightly bracket the Balmer and 4000A breaks of the protocluster galaxies. We find that two-thirds of the protocluster galaxies, which lie between several compact groups, are indistinguishable from field galaxies. The other third, which reside within the groups, differ significantly from the intergroup galaxies in both colour and specific star formation rate. We find that the fraction of red galaxies within the massive protocluster groups is twice that of the intergroup region. These excess red galaxies are due to enhanced fractions of both passive galaxies (1.7 times that of the intergroup region) and dusty star-forming galaxies (3 times that of the intergroup region). We infer that some protocl...

  14. Complexes of Peptide Blockers with Kv1.6 Pore Domain: Molecular Modeling and Studies with KcsA-Kv1.6 Channel.

    Science.gov (United States)

    Nekrasova, O V; Volyntseva, A D; Kudryashova, K S; Novoseletsky, V N; Lyapina, E A; Illarionova, A V; Yakimov, S A; Korolkova, Yu V; Shaitan, K V; Kirpichnikov, M P; Feofanov, A V

    2016-09-17

    Potassium voltage-gated Kv1.6 channel, which is distributed primarily in neurons of central and peripheral nervous systems, is of significant physiological importance. To date, several high-affinity Kv1.6-channel blockers are known, but the lack of selective ones among them hampers the studies of tissue localization and functioning of Kv1.6 channels. Here we present an approach to advanced understanding of interactions of peptide toxin blockers with a Kv1.6 pore. It combines molecular modeling studies and an application of a new bioengineering system based on a KcsA-Kv1.6 hybrid channel for the quantitative fluorescent analysis of blocker-channel interactions. Using this system we demonstrate that peptide toxins agitoxin 2, kaliotoxin1 and OSK1 have similar high affinity to the extracellular vestibule of the K(+)-conducting pore of Kv1.6, hetlaxin is a low-affinity ligand, whereas margatoxin and scyllatoxin do not bind to Kv1.6 pore. Binding of toxins to Kv1.6 pore has considerable inverse dependence on the ionic strength. Model structures of KcsA-Kv1.6 and Kv1.6 complexes with agitoxin 2, kaliotoxin 1 and OSK1 were obtained using homology modeling and molecular dynamics simulation. Interaction interfaces, which are formed by 15-19 toxin residues and 10 channel residues, are described and compared. Specific sites of Kv1.6 pore recognition are identified for targeting of peptide blockers. Analysis of interactions between agitoxin 2 derivatives with point mutations (S7K, S11G, L19S, R31G) and KcsA-Kv1.6 confirms reliability of the calculated complex structure.

  15. Recounting the History of the Albatross Award

    Science.gov (United States)

    Knauss, John; Lill, Gordon; Maxwell, Arthur

    The origin of the Albatross Award can be traced directly to a dinner party in early 1959 at the home of Gordon and Mildred Lill in the Virginia suburbs of Washington, D.C. Guests were Arthur Maxwell and his wife and John Knauss. Lill and Maxwell worked at the Office of Naval Research (ONR). Knauss was visiting from the Scripps Institution of Oceanography in LaJolla, California. During the evening the subject of the lack of awards and prizes for oceanographers arose. The three decided that perhaps this was a situation that could be remedied by the American Miscellaneous Society (AMSOC).

  16. [Pie]: Re-Count and Recall

    Science.gov (United States)

    Scott, Paul

    2008-01-01

    The number [pie] [approximately] 3.14159 is defined to be the ratio C/d of the circumference C to the diameter d of any given circle. In this article, the author looks at some surprising and unexpected places where [pie] occurs, and then thinks about some ways of remembering all those digits in the expansion of [pie].

  17. Contrastive Graduation Studies in Recount and Exposition

    Institute of Scientific and Technical Information of China (English)

    Simon Borg

    2010-01-01

    @@ 1 Introduction In this talk I provide a contemporary overview of the field of language teacher education (LTE). Following brief comments on the global and varied scope of LTE and its relatively recent history as a field of inquiry, I examine in turn a number of central themes highlighted in current LTE literature.

  18. HST 1.6μm Imaging Survey of Orion Protostars

    Science.gov (United States)

    Booker, Joseph J.; Megeath, S. Thomas; Fischer, William J.; Kounkel, Marina; Poteet, Charles A.; Furlan, Elise; Stutz, Amelia Marie; Puravankara, Manoj; Tobin, John J.; Nagy, Zsofia; Watson, Dan M.; Herschel Orion Protostar Survey

    2017-01-01

    We present near-infrared 1.6μm HST NICMOS and/or WFC3 images of 244 protostars in the Orion A & B molecular clouds, the largest sample of protostars imaged in a single cloud complex to date. These protostars are part of the Herschel Orion Protostar Survey (HOPS), a multi-observatory program which obtained 1-870μm photometry, spectroscopy, imaging of 319 protostars in the Orion clouds. The HST images resolve structures illuminated in scattered light from the central protostar, including disks, cavities, and shadows cast in envelopes by disks, with better than 100 AU spatial resolution. We classify all the protostars into five morphological classes: non-detections, point sources, bipolar cavities, unipolar cavities and irregular sources. Sixteen of the bipolar sources show disks in absorption, revealing a minimum spatial extent of the disks. The resolved cavities allow us to directly measure the clearing of the envelopes by bipolar outflows. We map cavities for 30 of these sources by applying a custom edge detection technique to both the scattered light images and radiative transfer models with known cavity geometries. We constrain the shape of the cavities and estimate the fractional volumes of the collapsing cores dispersed by the outflows. Contrary to previous results, we do not find evidence that outflow cavities grow in volume as protostars evolve from Class 0 to flat spectrum sources. These results indicate that feedback by outflow clearing is not the primary agent for dissipating envelopes and halting accretion, and cannot explain the 30-40% star formation efficiency estimated for molecular cores.

  19. Osteoblast biocompatibility of novel chitosan crosslinker, hexamethylene-1,6-diaminocarboxysulfonate.

    Science.gov (United States)

    Beringer, Laura T; Kiechel, Marjorie A; Komiya, Yuko; Donius, Amalie E; Habas, Raymond; Wegst, Ulrike G K; Schauer, Caroline L

    2015-09-01

    Chitosan is a naturally occurring polysaccharide, which has proven to be an attractive candidate for bone tissue engineering, due to its ability to promote osteoblast mineralization. Electrospinning has become a well-established cell scaffold processing technique, as it produces a high surface area to volume fibrous material that can mimic the three dimensionality of the extracellular matrix of a cell. In this study, we have investigated the osteoblast response to two different chemically crosslinked (hexamethylene-1,6-diaminocarboxysulfonate (HDACS) and genipin) electrospun chitosan scaffolds and their film counterparts in order to determine how material chemistry influences cellular behavior in conjunction with material topology. In addition, material properties of each fiber scaffold such as porosity and tensile strength were considered. MLO-A5 osteoblast cells grown on chitosan-HDACS scaffolds were found to display a more organized cellular network, along with significantly more filopodia extensions, compared to those grown on chitosan-genipin scaffolds. After 2 days of growth on chitosan-HDACS fibers, a higher level of alkaline phosphatase expression in MLO-A5 cells was reported compared to that of either chitosan-genipin fibers or films. These results indicate that not only chemistry, but also surface topology is an important effecter of cellular behavior. Ultimately, chitosan-HDACS fiber scaffolds provided an adequate substrate for osteoblast attachment and proliferation.

  20. 1,6-庚二炔类化合物的合成%Synthesis of 1,6-Heptadiyne Compounds

    Institute of Scientific and Technical Information of China (English)

    刘影; 冯永华; 戚剑锋; 张辰

    2011-01-01

    溴丙炔与含活性亚甲基的丙二酸酯反应,合成了14个4-取代-1,6-庚二炔类化合物,其结构经1H NMR确证;4-二苯基磷酰基-4-乙酯基-1,6-庚二炔未见文献报道,其结构经1H NMR,13C NMR,IR和HR-MS表征.%Fourteen 4-substituted-1,6-heptadiyne compounds were synthesized by the reaction be tween propagyl bromide and malonic ester with active methylene. The structures were confirmed by 1H NMR. The structure of 4-diphenylphosphoryl-4-ethoxycarbonyl-1,6-heptadiyne (new compound) was characterized by 1H NMR, 13C NMR, IR and HR-MS.

  1. On the LiCo{sub 2/3}Ni{sub 1/6}Mn{sub 1/6}O{sub 2} positive electrode material

    Energy Technology Data Exchange (ETDEWEB)

    Mahmoud, Abdelfattah [ECME, FST Marrakech, University Cadi Ayyad, BP549, Av. A. Khattabi, Marrakech (Morocco); Saadoune, Ismael, E-mail: saadoune1@yahoo.f [ECME, FST Marrakech, University Cadi Ayyad, BP549, Av. A. Khattabi, Marrakech (Morocco); Amarilla, Jose Manuel [Instituto de Ciencias de Materiales de Madrid, CSIC, c/Sor Juana Ines de la Cruz, 3, Cantoblanco, 28049 Madrid (Spain); Hakkou, Rachid [ECME, FST Marrakech, University Cadi Ayyad, BP549, Av. A. Khattabi, Marrakech (Morocco)

    2011-04-15

    Research highlights: LiCo{sub 2/3}Ni{sub 1/6}Mn{sub 1/6}O{sub 2} layered oxide, prepared by the combustion method, consists of particles with homogenous distribution. Rietveld refinement shows that the crystal structure of this cathode material presents no Li/Ni mixing. The best cycling performances were recorded when the upper cut off is fixed at 4.5 V corresponding to a solid solution domain. - Abstract: LiCo{sub 2/3}Ni{sub 1/6}Mn{sub 1/6}O{sub 2} layered oxide was synthesized by the combustion method that led to a crystalline phase with good homogeneity and low particles size. The structural properties of the prepared positive electrode material were investigated by performing XRD Rietveld refinement. Practically no Li/Ni mixing was detected evidencing that the studied compound adopts almost an ideal {alpha}-NaFeO{sub 2} type structure. The Li||LiCo{sub 2/3}Ni{sub 1/6}Mn{sub 1/6}O{sub 2} cell showed a discharge capacity of 199 mAh g{sup -1} when cycled in the 2.7-4.6 V potential range while the best cycling performances were recorded when the upper cut off is fixed at 4.5 V. Structural changes in Li{sub x}Co{sub 2/3}Ni{sub 1/6}Mn{sub 1/6}O{sub 2} with lithium electrochemical de-intercalation were studied using X-ray diffraction. This study clearly shows the existence of a solid solution domain in the 0.1 < x < 1.0 composition range while for x = 0.1, a new phase appears explaining the decrease of the electrochemical performance when the cell is cycled at high upper cut off voltage.

  2. Synthesis and properties of polycyclic quinones condensed with 1,6-methano[10]annulene

    Directory of Open Access Journals (Sweden)

    Shigeyasu Kuroda et al

    2007-01-01

    Full Text Available Two types of polycyclic quinones condensed with 1,6-methano[10]annulenes as type A: 1,6-methanonaphtho[2,3-c][10]annulene-7,12-dione 5a, and type B: 1,6-methanonaphtho[2,3-c][10]annulene-5,14-dione 18, bis(1,6-methano[10]annuleno[3,4-b; 3,4-g]anthracene-10,21-dione 20, 1,6-methanoanthraceno[2,3-c][10]annulene-5,16-dione 22, 1,6-methanotetraceno[2,3-c][10]annulene-6,17-dione 23, and 1,6-methano phenanthreno[2,3-c][10]annulene-5,6-dione 24 have been synthesized. The acene derivative 6 corresponding to that of 5a was synthesized by the reduction of quinone 5a. The physical, spectral, and chemical properties of these new compounds have been investigated.

  3. One-pot Synthesis of 14-Aryl-1,6,7,1 4-tetrahydrodibenzo-[a,i]acridine-1,6-dione in Ionic Liquid

    Institute of Scientific and Technical Information of China (English)

    LI Yuling; XU Xiaoping; SHI Daqing; JI Shunjun

    2009-01-01

    14-Aryl-1,6,7,14-tetrahydrodibenzo[a,i]acridine-l,6-diones have been synthesized in ionic liquid [bmim]BF4 (bmim=1-butyl-3-methylimidazolium) at room temperature.Particularly valuable features of this method include high yields of products,recyclable reaction media,broad substrate scope,short reaction time and operational simplicity.

  4. Organic-acid-assisted fabrication of low-cost Li-rich cathode material (Li[Li1/6Fe1/6Ni1/6Mn1/2]O2) for lithium-ion battery.

    Science.gov (United States)

    Zhao, Taolin; Chen, Shi; Li, Li; Zhang, Xiaoxiao; Wu, Huiming; Wu, Tianpin; Sun, Cheng-Jun; Chen, Renjie; Wu, Feng; Lu, Jun; Amine, Khalil

    2014-12-24

    A novel Li-rich cathode Li[Li1/6Fe1/6Ni1/6Mn1/2]O2 (0.4Li2MnO3-0.6LiFe1/3Ni1/3Mn1/3O2) was synthesized by a sol-gel method, which uses citric acid (SC), tartaric acid (ST), or adipic acid (SA) as a chelating agent. The structural, morphological, and electrochemical properties of the prepared samples were characterized by various methods. X-ray diffraction showed that single-phase materials are formed mainly with typical α-NaFeO2 layered structure (R3̅m), and the SC sample has the lowest Li/Ni cation disorder. The morphological study indicated homogeneous primary particles in good distribution size (100 nm) with small aggregates. The Fe, Ni, and Mn valences were determined by X-ray absorption near-edge structure analysis. In coin cell tests, the initial reversible discharge capacity of an SA electrode was 289.7 mAh g(-1) at the 0.1C rate in the 1.5-4.8 V voltage range, while an SC electrode showed a better cycling stability with relatively high capacity retention. At the 2C rate, the SC electrode can deliver a discharge capacity of 150 mAh g(-1) after 50 cycles. Differential capacity vs voltage curves were employed to further investigate the electrochemical reactions and the structural change process during cycling. This low-cost, Fe-based compound prepared by the sol-gel method has the potential to be used as the high capacity cathode material for Li-ion batteries.

  5. Acetato(N-[(E-1-(6-methyl-2-pyridylmethylidene]-2-{2-[(E-1-(6-methyl-2-pyridylmethylideneamino]phenethyl}anilinenickel(II perchlorate

    Directory of Open Access Journals (Sweden)

    Hong-In Lee

    2010-10-01

    Full Text Available In the title complex, [Ni(CH3COO(C28H26N4]ClO4, the NiII atom is coordinated by two imine N atoms and two pyridine N atoms of the N-[(E-1-(6-methyl-2-pyridylmethylidene]-2-(2-[(E-1-(6-methyl-2-pyridylmethylideneamino]phenethylaniline donor ligand and two O atoms of the acetate ion in a distorted octahedral coordination. The average Ni—N and Ni—O bond lengths are 2.131 (13 and 2.098 (11 Å, respectively. An intramolecular N—H...O interaction occurs. Relatively weak intermolecular C—H...O interactions between the ligands and the ClO4− ions result in a chain extending along the b axis.

  6. Ford Duratec - 1.6 l Ti-VCT engine; Der neue Ford-Duratec - 1,6-l-Ti-VCT-Motor

    Energy Technology Data Exchange (ETDEWEB)

    Kaufeld, H.; Koelsch, U.; Rechs, M.; Ruhland, H.; Springer, K.M. [Ford-Werke AG, Koeln (Germany)

    2005-03-01

    A new variable cam timing variant of the 1.6 l Duratec gasoline engine known from Fiesta and Focus has further enhanced efficiency compared to the base engine. This Ti-VCT engine (Twin Independent Variable Cam Timing) offers less consumption as well as improved torque characteristics and peak power. This report leads through the design of the new engine. Furthermore the thermodynamical development of this concept is introduced. (orig.)

  7. α1, 6-fucosyltransferase and tumor: recent progress%α-1,6岩藻糖基转移酶与肿瘤

    Institute of Scientific and Technical Information of China (English)

    季君; 高春芳

    2011-01-01

    Core fucose glycosylation catalyzed by α1, 6-fucosetransferase(Fut8) is an important way for protein posttranslational modification and functional regulation; it directly influences a series of biological characteristics of cells and is always seen under some pathological conditions. This paper reviews the recent studies on the regulation, biological function of Fut8 and its relationship with different kinds of tumors.%由α-1,6岩藻糖基转移酶(α1,6-fucosyltransferase,Fut8)催化的核心岩藻糖基化是糖蛋白重要的翻译后修饰和功能调控方式,直接影响细胞一系列生物学特性,而这种糖基化的异常改变往往是疾病状态的特点.本文就Fut8的表达调控特征、生物学功能及其与各种肿瘤的关系研究进展作一综述.

  8. Volume Entropy

    CERN Document Server

    Astuti, Valerio; Rovelli, Carlo

    2016-01-01

    Building on a technical result by Brunnemann and Rideout on the spectrum of the Volume operator in Loop Quantum Gravity, we show that the dimension of the space of the quadrivalent states --with finite-volume individual nodes-- describing a region with total volume smaller than $V$, has \\emph{finite} dimension, bounded by $V \\log V$. This allows us to introduce the notion of "volume entropy": the von Neumann entropy associated to the measurement of volume.

  9. VT Lidar nDSM (1.6 meter) - 2008 - West Franklin

    Data.gov (United States)

    Vermont Center for Geographic Information — (Link to Metadata) This metadata applies to the following collection area(s): Missisquoi Lower 2008 1.6m and related "normalized" Digital Surface Model (nDSM)....

  10. VT Lidar DSM (1.6 meter) - 2010 - East Franklin/West Orleans

    Data.gov (United States)

    Vermont Center for Geographic Information — (Link to Metadata) This metadata applies to the following collection area(s): Missisquoi Upper 2010 1.6m and related Digital Surface Model (DSM) data. This metadata...

  11. VT Lidar Hydro-flattened DEM (1.6 meter) - 2008 - West Franklin

    Data.gov (United States)

    Vermont Center for Geographic Information — (Link to Metadata) This metadata applies to the following collection area(s): Missisquoi Lower 2008 1.6m and Digital Elevation Model (DEM) datasets of various...

  12. VT Lidar nDSM (1.6 meter) - 2012 - Addison County

    Data.gov (United States)

    Vermont Center for Geographic Information — (Link to Metadata) This metadata applies to the following collection area(s): Addison County 2012 1.6m and related "normalized" Digital Surface Model (nDSM). Created...

  13. VT Lidar Hydro-flattened DEM (1.6 meter) - 2010 - East Franklin/West Orleans

    Data.gov (United States)

    Vermont Center for Geographic Information — (Link to Metadata) This metadata applies to the following collection area(s): Missisquoi Upper 2010 1.6m and Digital Elevation Model (DEM) datasets of various...

  14. Bacteriophage endolysin Lyt μ1/6: characterization of the C-terminal binding domain.

    Science.gov (United States)

    Tišáková, Lenka; Vidová, Barbora; Farkašovská, Jarmila; Godány, Andrej

    2014-01-01

    The gene product of orf50 from actinophage μ1/6 of Streptomyces aureofaciens is a putative endolysin, Lyt μ1/6. It has a two-domain modular structure, consisting of an N-terminal catalytic and a C-terminal cell wall binding domain (CBD). Comparative analysis of Streptomyces phage endolysins revealed that they all have a modular structure and contain functional C-terminal domains with conserved amino acids, probably associated with their binding function. A blast analysis of Lyt μ1/6 in conjunction with secondary and tertiary structure prediction disclosed the presence of a PG_binding_1 domain within the CBD. The sequence of the C-terminal domain of lyt μ1/6 and truncated forms of it were cloned and expressed in Escherichia coli. The ability of these CBD variants fused to GFP to bind to the surface of S. aureofaciens NMU was shown by specific binding assays.

  15. Graphene Functionalization by 1,6-Diaminohexane and Silver Nanoparticles for Water Disinfection

    National Research Council Canada - National Science Library

    Abdelhalim, Abdelsattar O. E; Galal, Ahmed; Hussein, Mohamed Z; El Sayed, Ibrahim E.-T

    2016-01-01

      Reduced graphene (G) was prepared by chemically reducing graphene oxide (GO). For the first time, the resulting G was functionalized by 1,6-diaminohexane and decorated with silver nanoparticles (AgNPs...

  16. VT Lidar Hydro-flattened DEM (1.6 meter) - 2012 - Addison

    Data.gov (United States)

    Vermont Center for Geographic Information — (Link to Metadata) This metadata applies to the following collection area(s): Addison County 2012 1.6m and Digital Elevation Model (DEM) datasets of various...

  17. Synthesis of 14-aryl-1,6,7,14-tetrahydrodibenzo [a,i]acridine-1,6-diones in PEG 600%PEG600反应介质中合成14-芳基-1,6,7,14-四氢二苯并[a,i]吖啶-1,6-二酮

    Institute of Scientific and Technical Information of China (English)

    梁一川; 史亚南; 杜百祥; 李玉玲

    2013-01-01

    在室温条件下以PEG 600为反应介质,三组分一锅法高效地合成了14-芳基-1,6,7,14-四氢二苯并[a,i]吖啶-1,6-二酮.该方法具有反应时间短、产率高、污染小、操作简单等优点.作为反应介质的PEG可以回收使用.%A facile one pot synthesis of 14-aryl-1,6,7,14-tetrahydrodibenzo[a,i]acridine-1,6-diones is accomplished via a three-component reaction in PEG 600 at room temperature.This method has the advantage of short reaction time,good yields,less pollution and simple reaction conditions.The PEG can be recovered and reused.

  18. VT Lidar Hillshade (1.6 meter) - 2010 - East Franklin/West Orleans

    Data.gov (United States)

    Vermont Center for Geographic Information — (Link to Metadata) This metadata applies to the following collection area(s): Missisquoi Upper 2010 1.6m and related "HILLSHADE" raster data. HILLSHADE data is for...

  19. Dietary Beta-1,3/1,6-Glucans Reduce Clinical Signs of Canine Atopy

    Directory of Open Access Journals (Sweden)

    A. C. Beynen

    2011-01-01

    Full Text Available Problem statement: There was evidence that beta-1,3/1,6-glucans modulate inflammatory activity. In an open, non-controlled trial, purified beta-1,3/1,6-glucans were found to improve the clinical signs of dogs with undefined chronic skin disorders. Given the design of that study, further work was required on the efficacy of beta-1,3/1,6-glucans in the treatment of canine atopy. Approach: The influence of a purified preparation of beta-1,3/1,6-glucans (MacroGard® on canine atopy was assessed in a double-blind, placebo-controlled trial. Privately owned dogs were used and the clinical signs of atopic dermatitis were evaluated by the owners. For a period of 8 weeks, the dogs daily received a complete dry food without (n = 16 or with 800 ppm beta-1,3/1,6-glucans (n = 15. During the trial, all dogs were treated three times with the use of a flea remedy in order to exclude any influence of flea-bite allergy. To assess the severity of atopic dermatitis, the clinical signs scored were itching, redness, scaling, thickening and stripping of skin. Results: For all five clinical signs, the group-mean improvement, expressed as change of severity score over time, was greater in the test group than in the controls. Within each group, the changes for the five clinical signs were added up to arrive at an overall index of improvement of atopic dermatitis. The extra improvement caused by the ingestion of beta-1,3/1,6-glucans was 63%. The difference between the pooled group-mean changes of the scores for the control and test dogs was statistically significant (PConclusion: Beta-1,3/1,6-glucans can be considered safe and it is put forward that a dose of 800 ppm in a dry food is beneficial for dogs with atopic dermatitis.

  20. Heterogeneous synthesis of dimethylhexane-1,6-dicarbamate from 1,6-hexanediamine and methyl carbonate in methanol over a CeO2 catalyst☆

    Institute of Scientific and Technical Information of China (English)

    Yan Cao; Huiquan Li; Xintao Li; Liguo Wang; Ganyu Zhu; Qing Tang

    2015-01-01

    The efficient synthesis of dimethylhexane-1,6-dicarbamate (HDC) from 1,6-hexanediamine (HDA) and methyl carbonate over a series of heterogeneous catalysts (e.g., MgO, Fe2O3, Mo2O3, and CeO2) was investigated. The reaction pathway was confirmed as an alcoholysis reaction through a series of designed experiments. Under optimized conditions, 100%HDA conversion with 83.1%HDCtotal and 16.9%polyurea was obtained using a one-step with high temperature procedure with CeO2 as the catalyst. A new two-step with variable temperature technol-ogy was developed based on the reaction pathway to reduce the polyurea yield. Using the proposed method, the HDCtotal yield reached 95.2%, whereas the polyurea yield decreased to 4.8%. The CeO2 catalyst showed high stability and did not exhibit any observable decrease in the HDC yield or any structural changes after four recycling periods. © 2014 The Chemical Industry and Engineering Society of China, and Chemical Industry Press. Al rights reserved.

  1. AMP makes native snake muscle fructose-1,6-bisphosphatase to an alkaline enzyme

    Institute of Scientific and Technical Information of China (English)

    赵辅昆; 徐松琴; 杜立林; 许根俊

    2000-01-01

    A substance in the crude preparation of NADP+ has been found, which activates snake muscle fructose-1,6-bisphosphatase at pH 9.2 and inhibits the enzyme at pH 7.5. After isolation and extensive characterization, the substance has been determined to be AMP. The activation depends on the concentrations of Mg2+ and could be observed only at concentrations above 1 mmol/L. In the presence of AMP, snake muscle fructose-1,6-bisphosphatase resembles an alkaline enzyme. Kinetic studies indicate that AMP and Mg2+ competitively regulate the activity of the enzyme. AMP releases the inhibition of Mg2+ at high concentration at alkaline pH. It has been reported that fructose-1,6-bisphosphatase with a pH optimum in the alkaline region is caused by limited proteolysis. AMP is also able to make fructose-1,6-bisphosphatase to be an alkaline enzyme. This finding indicates that proteolysis may not be the only reason for shift of the optimum pH of fructose-1,6-bisphosphatase to alkaline side and it may imply some significanc

  2. Graphene Functionalization by 1,6-Diaminohexane and Silver Nanoparticles for Water Disinfection

    Directory of Open Access Journals (Sweden)

    Abdelsattar O. E. Abdelhalim

    2016-01-01

    Full Text Available Reduced graphene (G was prepared by chemically reducing graphene oxide (GO. For the first time, the resulting G was functionalized by 1,6-diaminohexane and decorated with silver nanoparticles (AgNPs. The resulting G and modified G were characterized by X-ray diffraction (XRD, nuclear magnetic resonance (NMR, infrared (FTIR spectroscopies, and high resolution transmission electron microscopy (HRTEM. The 1,6-diaminohexane-graphene structure was ascertained from the NMR and FTIR data. AgNPs were identified with various sizes within the graphene matrix. The resulting hybrid material was used as an effective antimicrobial contact catalyst for disinfecting water from Total Coliform and Fecal Coliform bacteria. A triple action in this respect was achieved from graphene, 1,6-diaminohexane, and AgNPs without observed release of silver that causes toxicity.

  3. Genotoxic Potential of 1.6 GHz Wireless Communication Signal: In vivo Two-Year Bioassay

    Energy Technology Data Exchange (ETDEWEB)

    Vijayalaxmi, Vijay (University of Texas at San Antonio); Sasser, Lyle B.(SELF-EMPLOYED CONSULTANTS); Morris, J E.(GENERAL ELECTRIC CO.); Wilson, Bary W.(BATTELLE (PACIFIC NW LAB)); Anderson, Larry E.(SELF-EMPLOYED CONSULTANTS)

    2003-04-01

    Timed-pregnant Fischer 344 rats (from nineteenth day of gestation) and their nursing offspring (until weaning) were exposed to a far-field 1.6 GHz Iridium wireless communication signal for 2 h/day, 5 days/week. Far-field whole-body exposures were conducted with a field intensity of 0.43 mW/cm 2 and whole-body average specific absorption rate (SAR) of 0.036 to 0.077 W/kg (0.10 to 0.22 W/kg in the brain). This was followed by chronic, head-only exposures of male and female offspring to a near-field 1.6 GHz signal for 2 h/day, 5 days/week, over 2 years. Near-field exposures were conducted at an SAR of 0.16 or 1.6 W/kg in the brain. Concurrent sham-exposed and cage control rats were also included in the study. At the end of 2 years, all rats were necropsied. Bone marrow smears were examined for the extent of genotoxicity, assessed from the presence of micronuclei in polychromatic erythrocytes. The results indicated that the incidence of micronuclei/ 2000 polychromatic erythrocytes were not significantly different between 1.6 GHz-exposed, sham-exposed and cage control rats. The group mean frequencies were 5.6 6 1.8 (130 rats exposed to 1.6 GHz at 0.16 W/kg SAR), 5.4 6 1.5 (135 rats exposed to 1.6 GHz at 1.6 W/kg SAR), 5.6 6 1.7 (119 sham-exposed rats), and 5.8 6 1.8 (100 cage control rats). In contrast, positive control rats treated with mitomycin C exhibited significantly elevated incidence of micronuclei/2000 polychromatic erythrocytes in bone marrow cells; the mean frequency was 38.2 6 7.0 (five rats). Thus there was no evidence for excess genotoxicity in rats that were chronically exposed to 1.6 GHz compared to sham-exposed and cage controls.

  4. Disordered organic electronic materials based on non-benzenoid 1,6-methano[10]annulene rings

    Science.gov (United States)

    Tovar, John D; Streifel, Benjamin C; Peart, Patricia A

    2014-10-07

    Conjugated polymers and small molecules including the nonplanar aromatic 1,6-methano[10]annulene ring structure along with aromatic subunits, such as diketopyrrolopyrrole, and 2,1,3-benzothiadiazole, substituted with alkyl chains in a "Tail In," "Tail Out," or "No Tail" regiochemistry are disclosed.

  5. 1,6-Diaminoperylene bisimide with a highly twisted perylene core

    Indian Academy of Sciences (India)

    CHE-WEI CHANG; FANG-YUN CHIEN; JIUN-WEI HU; HSING-YANG TSAI; KEW-YU CHEN

    2017-02-01

    1,6-Diaminoperylene bisimide (1) was synthesized and characterized by single-crystal X-raydiffraction. To the best of our knowledge, this is the first time that the structure of 1,6-disubstituted perylene bisimide has been reported. The crystal belongs to triclinic, space group P-1, with a = 10.3966(10), b = 15.3398(16), c = 16.8495(17) Å, α = 79.490(4)◦, β = 87.055(3)◦, γ = 79.423(3)◦, and Z = 2. Compound1 possesses two intramolecular C–H· · ·N hydrogen bonds, which generate two S(6) ring motifs. The central perylene core of 1 is twisted with dihedral angles of 19.48(2)◦ and 19.50(2)◦; this twist configuration induces the axial chirality in this family of perylene bisimide chromophores. Density functional theory (DFT) calculations also show that the core twist angles of 1,6-diaminoperylene bisimide are larger than those of 1,7-diaminoperylene bisimide, which may account for the fact that the 1,7-regioisomer has a more extended effective conjugation length than the 1,6-regioisomer.

  6. [Fructose-1,6-bisphosphatase--marker of damage to proximal renal tubules].

    Science.gov (United States)

    Kepka, Alina; Szajda, Sławomir D; Zwierz, Krzysztof

    2008-02-01

    Pathological processes disturbing function of renal proximal tubules, increase activity of fructose-1,6-bisphosphatase (FBP-1) in urine. FBP-1 is cytosolic enzyme which occured mainly in cells of proximal renal tubules, and to small extent in cells of pars recta. After damage to the cell membrane FBP-1 is more rapidly excreted to the urine, than enzymes residing in other cell organelles. Fructose-1,6-bisphosphatase was isolated from rabbit muscle in 1943 by Gomori, and from spinach in 1958 by Racker i Schröder. Highest activity of FBP-1 was found in liver and kidneys, lesser in ileum, leucocytes, muscles and brain. Fructose-1,6-bisphosphatase is one of four key enzymes of gluconeogenesis performing synthesis of glucose from non sugar substrates. FBP-1 catalyses hydrolysis of fructose-1,6-bisphosphate in cytoplasm of the cell. There are many reports on properties and significance of FBP-1 in plant and animal tissues, but only few reports on activity of this enzyme in urine. Reason for little interest in determination of FBP-1 activity in urine, is relative instability of this enzyme in urine.

  7. Plant-wide assessment summary: $1.6 million in savings identified in Augusta Newsprint assessment

    Energy Technology Data Exchange (ETDEWEB)

    None, None

    2003-08-01

    Augusta Newsprint and its partners conducted a systematic plant-wide assessment (PWA) to identify energy- and cost-saving opportunities at the company's plant in Augusta, Georgia. The assessment team identified $1.6 million in potential annual savings.

  8. Modification of Rhizopus lactate dehydrogenase for improved resistance to fructose 1,6-bisphosphate

    Science.gov (United States)

    Rhizopus oryzae is frequently used for fermentative production of lactic acid. We determined that one of the key enzymes, lactate dehydrogenase (LDH), involved in synthesis of lactic acid by R. oryzae was significantly inhibited by fructose 1,6-bisphosphate (FBP) at physiological concentrations. Thi...

  9. Magnetic entropy change and magnetic phase transition of LaFe11.4Al1.6Cx(x=0-0.8)compounds

    Institute of Scientific and Technical Information of China (English)

    Chen Jing; Zhang Hong-Wei; Zhang Li-Gang; Dong Qiao-Yan; Wang Ru-Wu

    2006-01-01

    The unit cell volume and phase transition temperature of LaFe11.4Al1.6Cx compounds have been studied.The magnetic entropy change,refrigerant capacity and the type of magnetic phase transition are investigated in detail for LaFe11.4Al1.6Cx with x=0.1.All the LaFe11.4Al1.6Cx(x=0-0.8)compounds have the cubic NaZn13-type structure.The addition of carbon atoms brings about a considerable increase in the lattice parameter.The bulk expansion results in the change of phase transition temperature(Tc).Tc increases from 187 K to 269 K with x varying from 0.1 to 0.8.Meanwhile an increase in the lattice parameter can also cause a change of the magnetic ground state from antiferromagnetic to ferromagnetic.Large magnetic entropy change |△S| is found over a large temperature range around Tc and the refrigerant capacity is about 322J/kg for LaFe11.4Al1.6C0.1.The magnetic phase transition belongs in weakly first-order one for x=0.1.

  10. ADAM low- and medium-resolution spectrograph for 1.6-m AZT-33IK telescope

    CERN Document Server

    Afanasiev, V L; Amirkhanyan, V R; Moiseev, A V

    2016-01-01

    We describe the design of a low- and medium-resolution spectrograph ( R=300-1300) developed at the Special Astrophysical Observatory of the Russian Academy of Sciences (SAO RAS) for the 1.6-m AZT-33IK telescope of Sayan Observatory of the Institute of Solar-Terrestrial Physics of the Siberian Branch of the Russian Academy of Sciences. We report the results of laboratory measurements of the parameters of the instrument and tests performed on the SAO RAS 1-m Zeiss-1000 telescope. We measured the total quantum efficiency of the "spectrograph + telescope + detector" system on AZT-33IK telescope, which at its maximum reaches 56%. Such a hight transparency of the spectrograph allows it to be used with the 1.6-m telescope to determine the types and redshifts of objects with magnitudes m_AB~20-21, that was confirmed by actual observations.

  11. A New Method of Crystallization of Octahydro Trisodium Salt of Fructose-1,6-diphosphate

    Institute of Scientific and Technical Information of China (English)

    应汉杰; 欧阳平凯

    2002-01-01

    In order to overcome the elementary heterogeneous nucleation while octahydro trisodium salt of fructose-1,6-diphosphate(FDPNaa.8 H2O) is crystallized with ethanol precipitation at low temperature, a new crystallizationmethod with alcohol precipitation combined with salt precipitation has been presented. The ethanol-sodium ac-etate system for crystallization of salt of fructose-1,6-diphosphate is based on the mechanism of crystallization ofFDPNa3.8 H2O in the ethanol-low temperature system. It is found that crystal size may be controlled by regulatingtemperature or pH value of solution in the crystallization process, and the crystal yield increases to 95% from 78%which obtained in the ethanol-low temperature system.

  12. A spectroscopic study of a z=1.6 galaxy overdensity with GMASS

    CERN Document Server

    Kurk, Jaron; Zamorani, Gianni; Halliday, Claire; Mignoli, Marco; Pozzetti, Lucia; Daddi, Emanuele; Rosati, Piero; Dickinson, Marc; Bolzonella, Micol; Cassata, Paolo; Renzini, Alvio; Franceschini, Alberto; Rodighiero, Giulia; Berta, Stefano

    2008-01-01

    The Galaxy Mass Assembly ultra-deep Spectroscopic Survey samples a part of the CDFS to unprecedented depth. The resulting distribution of 150 z>1.4 redshifts reveals a significant peak at z=1.6, part of a larger overdensity found at this redshift. The 42 spectroscopic members of this structure, called Cl 0332-2742, form an overdensity in redshift of a factor 11+/-3 and have a velocity dispersion of 450 km/s. We derive a total mass for Cl 0332-2742 of ~7x10^14 Msol. The colours of its early-type galaxies are consistent with a theoretical red sequence of galaxies with stars formed at z=3.0. In addition, there are more massive, passive and older, but less star forming galaxies in CL 0332-2742 than in the field. We conclude that this structure is a cluster under assembly at z=1.6.

  13. 1,1′-{(Hexane-1,6-diylbis[(azaniumylylidenemethanylylidene]}bis(naphthalen-2-olate

    Directory of Open Access Journals (Sweden)

    Kamel Ouari

    2015-01-01

    Full Text Available The whole molecule of the title Schiff base compound, C28H28N2O2, is generated by inversion symmetry. It is formed from two units of ortho-hydroxynaphthaldehyde bridged with 1,6-diaminohexane. The N atoms are protonated and, thus, the structure is a bis-zwitterionic compound in the solid state. The zwitterion shows strong intramolecular N—H...O hydrogen bonds between the iminium N and the naphthalenolate O atoms.

  14. Stabilizing the urinary activity of fructose-1,6-bisphosphatase with EDTA and mercaptoethanol.

    Science.gov (United States)

    Kepka, Alina; Szajda, Sławomir Dariusz; Waszkiewicz, Napoleon; Zalewska, Anna; Płudowski, Paweł; Zwierz-Gugała, Dorota; Zalewska-Szajda, Beata; Borzym-Kluczyk, Małgorzata; Kryśkiewicz, Edyta; Zwierz, Krzysztof

    2009-09-01

    The study aim was to establish conditions for stabilization the activity of fructose-1,6-bisphosphatase (FBP-1) in stored urine. The FBP-1 was determined by the method Kepka et. al in a collected fraction of purified urine. EDTA and mercaptoethanol stabilized FBP-1 activity in stored urine. At optimal conditions urine may be stored up to 7 days at a temperature of 4 degrees C.

  15. Sub-two-cycle light pulses at 1.6 microm from an optical parametric amplifier.

    Science.gov (United States)

    Brida, D; Cirmi, G; Manzoni, C; Bonora, S; Villoresi, P; De Silvestri, S; Cerullo, G

    2008-04-01

    We generate ultrabroadband pulses, spanning the 1200-2100 nm wavelength range, from an 800 nm pumped optical parametric amplifier (OPA) working at degeneracy. We compress the microjoule-level energy pulses to nearly transform-limited 8.5 fs duration by an adaptive system employing a deformable mirror. To our knowledge, these are the shortest light pulses generated at 1.6 microm.

  16. Synthesis, antifungal activity, and QSAR studies of 1,6-dihydropyrimidine derivatives

    Directory of Open Access Journals (Sweden)

    Chirag Rami

    2013-01-01

    Full Text Available Introduction: A practical synthesis of pyrimidinone would be very helpful for chemists because pyrimidinone is found in many bioactive natural products and exhibits a wide range of biological properties. The biological significance of pyrimidine derivatives has led us to the synthesis of substituted pyrimidine. Materials and Methods: With the aim of developing potential antimicrobials, new series of 5-cyano-6-oxo-1,6-dihydro-pyrimidine derivatives namely 2-(5-cyano-6-oxo-4-substituted (aryl-1,6-dihydropyrimidin-2-ylthio-N-substituted (phenyl acetamide (C1-C41 were synthesized and characterized by Fourier transform infrared spectroscopy (FTIR, mass analysis, and proton nuclear magnetic resonance ( 1 H NMR. All the compounds were screened for their antifungal activity against Candida albicans (MTCC, 227. Results and Discussion: Quantitative structure activity relationship (QSAR studies of a series of 1,6-dihydro-pyrimidine were carried out to study various structural requirements for fungal inhibition. Various lipophilic, electronic, geometric, and spatial descriptors were correlated with antifungal activity using genetic function approximation. Developed models were found predictive as indicated by their square of predictive regression values (r 2pred and their internal and external cross-validation. Study reveals that CHI_3_C, Molecular_SurfaceArea, and Jurs_DPSA_1 contributed significantly to the activity along with some electronic, geometric, and quantum mechanical descriptors. Conclusion: A careful analysis of the antifungal activity data of synthesized compounds revealed that electron withdrawing substitution on N-phenyl acetamide ring of 1,6-dihydropyrimidine moiety possess good activity.

  17. Fragrance material review on 2-cyclohexyl-1,6-heptadien-3-one.

    Science.gov (United States)

    Scognamiglio, J; Letizia, C S; Api, A M

    2013-12-01

    A toxicologic and dermatologic review of 2-cyclohexyl-1,6-heptadien-3-one when used as a fragrance ingredient is presented. 2-Cyclohexyl-1,6-heptadien-3-one is a member of the fragrance structural group Alkyl Cyclic Ketones. These fragrances can be described as being composed of an alkyl, R1, and various substituted and bicyclic saturated or unsaturated cyclic hydrocarbons, R2, in which one of the rings may include up to 12 carbons. Alternatively, R2 may be a carbon bridge of C2-C4 carbon chain length between the ketone and cyclic hydrocarbon. This review contains a detailed summary of all published and unpublished toxicology and dermatology papers that are related to this individual fragrance ingredient and is not intended as a stand-alone document. Available data for 2-cyclohexyl-1,6-heptadien-3-one were evaluated then summarized and includes physical properties, acute toxicity, skin irritation, mucous membrane (eye) irritation, skin sensitization, phototoxicity, photoallergy, repeated dose, and genotoxicity data. A safety assessment of the entire Alkyl Cyclic Ketones will be published simultaneously with this document; please refer to Belsito et al., 2013 for an overall assessment of the safe use of this material and all Alkyl Cyclic Ketones in fragrances. Copyright © 2013 Elsevier Ltd. All rights reserved.

  18. Inferno: June 1943 to May 1945. Book 5. Holocaust Series, First Edition.

    Science.gov (United States)

    Ayer, Eleanor H.

    There are eight volumes in this series on the Holocaust. Volumes 1-6 recount the history of the Jewish people in various year groupings: (1) "Ancient 1935"; (2) "September 1935 to December 1938"; (3) "January 1939 to December 1941"; (4) "January 1942 to June 1943"; (5) "July 1943 to April 1945"; (6) "May 1945 and After." Volumes 7 and 8 provide…

  19. File list: Oth.Emb.05.AllAg.1-6h_embryos [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Emb.05.AllAg.1-6h_embryos dm3 TFs and others Embryo 1-6h embryos SRX033315,SRX0...26864 http://dbarchive.biosciencedbc.jp/kyushu-u/dm3/assembled/Oth.Emb.05.AllAg.1-6h_embryos.bed ...

  20. File list: Unc.Emb.20.AllAg.1-6h_embryos [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Unc.Emb.20.AllAg.1-6h_embryos dm3 Unclassified Embryo 1-6h embryos SRX026866,SRX026...865,SRX033316,SRX033317 http://dbarchive.biosciencedbc.jp/kyushu-u/dm3/assembled/Unc.Emb.20.AllAg.1-6h_embryos.bed ...

  1. Catalytic enantioselective 1,6-conjugate additions of propargyl and allyl groups

    Science.gov (United States)

    Meng, Fanke; Li, Xiben; Torker, Sebastian; Shi, Ying; Shen, Xiao; Hoveyda, Amir H.

    2016-09-01

    Conjugate (or 1,4-) additions of carbanionic species to α,β-unsaturated carbonyl compounds are vital to research in organic and medicinal chemistry, and there are several chiral catalysts that facilitate the catalytic enantioselective additions of nucleophiles to enoates. Nonetheless, catalytic enantioselective 1,6-conjugate additions are uncommon, and ones that incorporate readily functionalizable moieties, such as propargyl or allyl groups, into acyclic α,β,γ,δ-doubly unsaturated acceptors are unknown. Chemical transformations that could generate a new bond at the C6 position of a dienoate are particularly desirable because the resulting products could then be subjected to further modifications. However, such reactions, especially when dienoates contain two equally substituted olefins, are scarce and are confined to reactions promoted by a phosphine-copper catalyst (with an alkyl Grignard reagent, dialkylzinc or trialkylaluminium compounds), a diene-iridium catalyst (with arylboroxines), or a bisphosphine-cobalt catalyst (with monosilyl-acetylenes). 1,6-Conjugate additions are otherwise limited to substrates where there is full substitution at the C4 position. It is unclear why certain catalysts favour bond formation at C6, and—although there are a small number of catalytic enantioselective conjugate allyl additions—related 1,6-additions and processes involving a propargyl unit are non-existent. Here we show that an easily accessible organocopper catalyst can promote 1,6-conjugate additions of propargyl and 2-boryl-substituted allyl groups to acyclic dienoates with high selectivity. A commercially available allenyl-boron compound or a monosubstituted allene may be used. Products can be obtained in up to 83 per cent yield, >98:2 diastereomeric ratio (for allyl additions) and 99:1 enantiomeric ratio. We elucidate the mechanistic details, including the origins of high site selectivity (1,6- versus 1,4-) and enantioselectivity as a function of the catalyst

  2. Interventions by Mothers of 1-6 Year Old Children after Home Accidents

    Directory of Open Access Journals (Sweden)

    Belkis Karatas

    2006-12-01

    Full Text Available PURPOSE: The purpose of the research was to determine the interventions after home accidents by women with a child between 1-6 years old.INSTRUMENT AND METHODS: The research was conducted as a descriptive study in the neighborhoods served by Çukurova Health Clinic affiliated with Mersin province center municipality. Using a simple random sampling method 100 women were taken into the sample by numbering the 1-6 year old child monitoring records. Data were collected on a questionnaire. Percentage distribution and Chi square test were used in the analysis of data obtained in the research.FINDINGS: The children of 66.0% of the women had had at least one home accident and the most frequent of the accidents was falling (66.7% followed by burns (43.9%. The majority of women, following their child's fall (61.4% applied a wound ointment and took the child to a health care facility and following a burn to their child 44.8% of the women used a cold application and took the child to a health care facility. The overwhelming majority of the women learned about first aid for childhood accidents from the people close to them, such as a relative or neighbor. There was no statistically significant difference between the women's sociodemographic characteristics and frequency of home accidents and the kind of first aid used for home accidents (P>0.05. RESULTS: The frequency of accidents in the homes of children between 1-6 years was high, and the majority of the first aid treatments administered by women following an accident was correct. Although the percentage was small some of the women did use inappropriate procedures.Keywords: Accident, injury, home accidents, childhood accidents

  3. Interventions by Mothers of 1-6 Year Old Children after Home Accidents

    Directory of Open Access Journals (Sweden)

    Belkis Karatas

    2006-12-01

    Full Text Available PURPOSE: The purpose of the research was to determine the interventions after home accidents by women with a child between 1-6 years old.INSTRUMENT AND METHODS: The research was conducted as a descriptive study in the neighborhoods served by Çukurova Health Clinic affiliated with Mersin province center municipality. Using a simple random sampling method 100 women were taken into the sample by numbering the 1-6 year old child monitoring records. Data were collected on a questionnaire. Percentage distribution and Chi square test were used in the analysis of data obtained in the research.FINDINGS: The children of 66.0% of the women had had at least one home accident and the most frequent of the accidents was falling (66.7% followed by burns (43.9%. The majority of women, following their child's fall (61.4% applied a wound ointment and took the child to a health care facility and following a burn to their child 44.8% of the women used a cold application and took the child to a health care facility. The overwhelming majority of the women learned about first aid for childhood accidents from the people close to them, such as a relative or neighbor. There was no statistically significant difference between the women's sociodemographic characteristics and frequency of home accidents and the kind of first aid used for home accidents (P>0.05. RESULTS: The frequency of accidents in the homes of children between 1-6 years was high, and the majority of the first aid treatments administered by women following an accident was correct. Although the percentage was small some of the women did use inappropriate procedures.Keywords: Accident, injury, home accidents, childhood accidents 

  4. Instanton Corrections of 1/6 BPS Wilson Loops in ABJM Theory

    CERN Document Server

    Okuyama, Kazumi

    2016-01-01

    We study instanton corrections to the vacuum expectation value (VEV) of 1/6 BPS Wilson loops in ABJM theory from the Fermi gas approach. We mainly consider Wilson loops in the fundamental representation and winding Wilson loops, but we also initiate the study of Wilson loops with two boundaries. We find that the membrane instanton corrections to the Wilson loop VEV are determined by the refined topological string in the Nekrasov-Shatashvili limit, and the pole cancellation mechanism between membrane instantons and worldsheet instantons works also in the Wilson loop VEVs as in the case of the partition functions.

  5. Enzymatic production of fructose 1,6-diphosphate using crude cell extract of Bacillus stearothermophilus.

    Science.gov (United States)

    Widjaja, A; Yasuda, M; Ogino, H; Nakajima, H; Ishikawa, H

    1999-01-01

    The enzymatic production of fructose 1,6-diphosphate (FDP) from glucose was performed in a batch reactor and a semibatch reactor using the crude cell extract of Bacillus stearothermophilus which contains all four enzymes required for the synthesis. The experimental results of the yield and the time courses of FDP production obtained using various enzyme concentrations were in good agreement with the theoretical predictions calculated based on the differential equations including the rate equations of the four enzymes, which were determined using the purified enzymes of B. stearothermophilus.

  6. Vibrational spectra of (BaF2)n (n=1-6) clusters

    Science.gov (United States)

    Pandey, Ratnesh K.; Waters, Kevin; Nigam, Sandeep; Pandey, Ravindra; Pandey, Avinash C.

    2016-05-01

    The vibrational properties of alkaline-earth metal fluoride clusters (BaF2)n (n=1-6) are investigated in the framework of density functional theory. The calculated Raman and Infrared (IR) spectra reveals shift in Raman and IR peak position towards lower frequency region with the increase in the cluster size. Further the calculated spectra have been compared with the experimental vibrational spectra of bulk BaF2 crystal. Even though the smaller size cluster lacks translational symmetry, the structural and vibrational characteristic of (BaF2)5-6 are nearer to bulk counterpart.

  7. Adsorption Behavior of CH2 and CH3 on Metal Clusters Cun (n=1-6)

    Institute of Scientific and Technical Information of China (English)

    Xi-hui Cheng; Ming-xing Jin; Zhan Hu; Fei-fei Hu; Da-jun Ding

    2008-01-01

    Using density functional theory with generalized gradient approximation and hybrid functional, we studied the properties of energy, charge population, and vibration of CH2 and CH3 adsorbed on Cun (n=1-6)clusters. The results show that the DFT calculation with the hybrid functional matches the experimental results better in both cases. The calculation results indicate that the adsorption of CH2 is stronger than that of CH3. During adsorption, the charges transfer from Cu to CH2 or CH3. The obtained vibrational frequencies for different modes of CH2 and CH3 adsorbed on Cun agree well with the experimental results for the adsorption on Cu(111) surface.

  8. 1.6 GHz VLBI Observations of SN 1979C: almost-free expansion

    CERN Document Server

    Marcaide, J M; Pérez-Torres, M A; Alberdi, A; Guirado, J C; Ros, E; Weiler, K W

    2009-01-01

    We report on 1.6 GHz Very-Long-Baseline-Interferometry (VLBI) observations of supernova SN 1979C made on 18 November 2002. We derive a model-dependent supernova size. We also present a reanalysis of VLBI observations made by us on June 1999 and by other authors on February 2005. We conclude that, contrary to our earlier claim of strong deceleration in the expansion, SN 1979C has been undergoing almost-free expansion ($m = 0.91\\pm0.09$; $R \\propto t^m$) for over 25 years.

  9. Design and Measurement of Dipole Magnets for CSNS 1.6GeV RCS

    CERN Document Server

    Li, Qing; Kang, Wen; Deng, Chang-dong; Chen, Wan

    2016-01-01

    The Rapid Cycling Synchrotron (RCS) in Chinese Spallation Neutron Source (CSNS) accelerates proton beam from 80Mev to 1.6GeV at a repetition rate of 25Hz. All dipole magnets of RCS are operated at AC with biased DC. Aiming at the properties of these dipole magnets, we take some methods to improve magnetic field quality in the good region and reduce eddy currents in the iron core . In this paper, we would present the process of the magnet design and temperature rise calculation. At the same time, the field measurement results and temperature test of the prototype magnet are also described and discussed.

  10. Superconductivity of Bi1.6Pbo.4Sr2Ca3Cu4O12

    Institute of Scientific and Technical Information of China (English)

    Atilla Coskun; Ahmet Ekicibil; Bekir Ozgelik

    2002-01-01

    The superconducting ceramics Bi1.6Pbo.4Sr2Ca3Cu4O12 have been prepared by the melt-casting method. A zero resistance temperature at 60 K has been observed. It has been found that the superconducting phase temperature Tc increases with increasing sintering temperature. The effect of Pb content on the superconductivity of the ceramic has been studied. The microstructure of the samplehas been investigated by scanning electron microscopy. Phase analysis has been carried out by x-ray diffraction patterns and energy dispersive analysis through x-ray spectroscopy.

  11. Nutrition Status Of Children (1-6 Years In Slums Of Ghaziabad City

    Directory of Open Access Journals (Sweden)

    Garg S.K

    1997-01-01

    Full Text Available Research question : What are the nutritional problems of pre- school children in slums? Objectives: (i To assess the nutritional status of the children . (ii To find out the nutritional deficiency disorders in them (iii To study their dietary intake. Study design: Cross- sectional. Setting : Slums of Ghaziabad city. Participants :771 children (1-6 years. Study Variables : Age, sex, caste, ICDS beneficiary status, weight, nutritional deficiency disorders, dietary intake and supplementary nutrition. Statistical analysis : Simple proportions and Chi- square test. Results : A majority (58.2 % of children were having under nutrition of varying grades irrespective of their sex and caste but influenced by their age and ICDS beneficiary status. Anaemia, xerophthalmia and goitre were present in 14.7%, 1.6% and 0.6 % children respectively. Average daily dietary intake of energy & nutrients were lower than the recommended daily allowances (RDA. Conclusion: Regular nutritional supplementation along with adequate nutrition education would reduce the nutritional deficiency disorders among children.

  12. Identification and characterization of cytosolic fructose-1,6-bisphosphatase in Euglena gracilis.

    Science.gov (United States)

    Ogawa, Takahisa; Kimura, Ayako; Sakuyama, Harumi; Tamoi, Masahiro; Ishikawa, Takahiro; Shigeoka, Shigeru

    2015-01-01

    Euglena gracilis has the ability to accumulate a storage polysaccharide, a β-1,3-glucan known as paramylon, under aerobic conditions. Under anaerobic conditions, E. gracilis cells degrade paramylon and synthesize wax esters. Cytosolic fructose-1,6-bisphosphatase (FBPase) appears to be a key enzyme in gluconeogenesis and position branch point of carbon partitioning between paramylon and wax ester biosynthesis. We herein identified and characterized cytosolic FBPase from E. gracilis. The Km and Vmax values of EgFBPaseIII were 16.5 ± 1.6 μM and 30.4 ± 7.2 μmol min(-1) mg protein(-1), respectively. The activity of EgFBPaseIII was not regulated by AMP or reversible redox modulation. No significant differences were observed in the production of paramylon in transiently suppressed EgFBPaseIII gene expression cells by RNAi (KD-EgFBPaseIII); nevertheless, FBPase activity was markedly decreased in KD-EgFBPaseIII cells. On the other hand, the growth of KD-EgFBPaseIII cells was slightly higher than that of control cells.

  13. K 1-6: an asymmetric planetary nebula with a binary central star

    CERN Document Server

    Frew, David J; Fitzgerald, Michael; Parker, Quentin; Danaia, Lena; McKinnon, David; Guerrero, Martín A; Hedberg, John; Hollow, Robert; An, Yvonne; Bor, Shu Han; Colman, Isabel; Graham-White, Claire; Li, Qing Wen; Mai, Juliette; Papadakis, Katerina; Picone-Murray, Julia; Hoang, Melanie Vo; Yean, Vivian

    2010-01-01

    We present new imaging data and archival multiwavelength observations of the little studied emission nebula K 1-6 and its central star. Narrow-band images in H-alpha (+ [NII]) and [OIII] taken with the Faulkes Telescope North reveal a stratified, asymmetric, elliptical nebula surrounding a central star which has the colours of a late G- or early K-type subgiant or giant. GALEX ultraviolet images reveal a very hot subdwarf or white dwarf coincident in position with this star. The cooler, optically dominant star is strongly variable with a period of 21.312 +/- 0.008 days, and is possibly a high amplitude member of the RS CVn class, although an FK Com classification is also possible. Archival ROSAT data provide good evidence that the cool star has an active corona. We conclude that K 1-6 is most likely an old bona fide planetary nebula at a distance of ~1.0 kpc, interacting with the interstellar medium, and containing a binary or ternary central star. The observations and data analyses reported in this paper wer...

  14. 2004 Molecular Basis of Microbial One-Carbon Metabolism Gordon Conference - August 1-6, 2004

    Energy Technology Data Exchange (ETDEWEB)

    Joseph A. Krzycki

    2005-09-15

    The Gordon Research Conference (GRC) on 2004 Molecular Basis of Microbial One-Carbon Metabolism Gordon Conference - August 1-6, 2004 was held at Mount Holyoke College, South Hadley, MA from August 1-6, 2004. The Conference was well-attended with 117 participants (attendees list attached). The attendees represented the spectrum of endeavor in this field coming from academia, industry, and government laboratories, both U.S. and foreign scientists, senior researchers, young investigators, and students. In designing the formal speakers program, emphasis was placed on current unpublished research and discussion of the future target areas in this field. There was a conscious effort to stimulate lively discussion about the key issues in the field today. Time for formal presentations was limited in the interest of group discussions. In order that more scientists could communicate their most recent results, poster presentation time was scheduled. Attached is a copy of the formal schedule and speaker program and the poster program. In addition to these formal interactions, 'free time' was scheduled to allow informal discussions. Such discussions are fostering new collaborations and joint efforts in the field.

  15. Hemodynamic effects of fructose 1,6-diphosphate in patients with normal and impaired left ventricular function.

    Science.gov (United States)

    Markov, A K; Brumley, M A; Figueroa, A; Skelton, T N; Lehan, P H

    1997-05-01

    We compared the short-term hemodynamic effects of intravenous fructose 1,6-diphosphate (FDP) administration in patients with coronary artery disease. Hemodynamic measurements were performed before and after administration of FDP in two groups of patients: those with impaired left ventricular (LV) function, elevated LV end-diastolic pressures (LVEDP > or =12 mm Hg, n = 30), and those with normal LV function (LVEDP <12 mm Hg, n = 17). In those with impaired LV function, FDP induced a decrease in LVEDP from 22 +/- 1.31 to 16.73 +/- 1.46 mm Hg (p< 0.0001). The cardiac index increased (2.50 +/- 0.11 to 2.81 +/- 0.13 L/m2 [p < 0.0001]), as did the LV stroke work index (31.7 +/- 2.04 to 40.3 +/- 2.67 gm x m x m2 [p < 0.0001]). FDP induced no significant change in heart rate and mean aortic pressure. Pulmonary pressure and resistance declined (p<0.002 and p< 0.0001, respectively). Systemic vascular resistance decreased because of increased cardiac output and unchanged arterial pressure (p < 0.001). In those patients with normal baseline LVEDP (5.06 +/- 0.27 mm Hg), FDP decreased heart rate (p< 0.0001) and systemic and pulmonary resistance (p < 0.03 and p < 0.004, respectively), whereas LVEDP and mean aortic and pulmonary pressures remained unchanged. FDP moderately increased cardiac output (p < 0.05), stroke volume index, and LV stroke work index (p< 0.002 and p< 0.003, respectively). The observed improvement in LV function in those patients with elevated LV filling pressures is thought to be a result of an increased energy production by the Embden-Meyerhoff pathway and to act as a positive inotrope.

  16. Synthesis and cytotoxic activity of carboxamide derivatives of benzo[b][1,6]naphthyridines.

    Science.gov (United States)

    Deady, Leslie W; Rodemann, Thomas; Zhuang, Li; Baguley, Bruce C; Denny, William A

    2003-03-13

    The reaction of 4-dimethylaminomethylene-6-methyl-4H-pyrano[4,3-b]quinoline-1,3-dione with a range of primary amines gave rise to a series of 2-substituted 6-methyl-1-oxo-1,2-dihydrobenzo[b][1,6]naphthyridine-4-carboxylic acids. The derived 4-N-[2-(dimethylamino)ethyl]carboxamides were tested for growth inhibitory properties against murine P388 leukemia, Lewis lung carcinoma (LLTC), and human Jurkat leukemia cell lines. Most compounds were potent cytotoxins, with some having IC(50) values less than 10 nM. Five were tested in vivo against subcutaneous colon 38 tumors in mice, and a single dose (3.9 mg/kg) proved to be curative for the 2-methyl and 2-(3,4-dimethoxyphenyl) derivatives in this refractory model.

  17. Magnetic refrigeration down to 1.6 K for the future circular collider e$^+$e$^-$

    CERN Document Server

    Tkaczuk, Jakub; Millet, Francois; Rousset, Bernard; Duval, Jean Marc

    2017-01-01

    High-field superconducting rf cavities of the future circular collider e+e− may require a kW-range superfluid helium refrigeration down to 1.6 K. Magnetic refrigeration operating below 4.2 K can be an alternative to the compression/expansion helium refrigeration. A significant difference between this application and previous magnetic refrigerator studies is its large cooling power, up to 103 times larger than the other designs. Principles of magnetic refrigeration are described and various technical solutions are compared. A numerical model for the static magnetic refrigerator is presented, validated, and adapted to the needs of the positron-electron version of the future circular collider. A preliminary design of magnetic refrigerator suitable for low temperature, kW-range cooling is studied.

  18. TRANSLOCACION NUCLEAR DE LA ISOFORMA HEPATICA DE FRUCTOSA 1,6-BISFOSFATASA

    OpenAIRE

    BERTINAT, ROMINA PAOLA

    2010-01-01

    El hígado cumple una función clave en la homeostasis de la glucosa. Gluconeogénesis es la vía metabólica encargada de la producción endógena de glucosa y es regulada a nivel de 3 enzimas que catalizan reacciones irreversibles, entre ellas fructosa 1,6-bisfosfatasa (FBPasa), cuya actividad es modulada por los inhibidores AMP y f2,6Pz. Glucosa e insulina inducen la concentración nuclear de FBPasa en hepatocitos aislados de rata; sin embargo, FBPasa de hígado de rata poseé 25 residuos extra en s...

  19. Survey of the occurrence of 1,6-hexanediol diglycidyl ether in food contact materials

    DEFF Research Database (Denmark)

    This project is a survey of the occurence of 1,6-hexanediol diglycidyl ether (HDDGE) specifically in food contact material on the Danish market This survey clarifies that HDDGE is listed on an inventory for possible use in printing inks food contact materials, but that is not used by European...... producers and importers. The substance is not allowed for use in plastic materials for food contact. One Danish company reported a possible use of HDDGE in coating of drinking tanks and pipelines. This is the only use of HDDGE confirmed in relation to food contact materials in Denmark. The project...... is following up on a previous survey under the Danish EPA’s LOUS-review (Environmental Project no. 1472)....

  20. Concept Development of a Mach 1.6 High-Speed Civil Transport

    Science.gov (United States)

    Shields, Elwood W.; Fenbert, James W.; Ozoroski, Lori P.; Geiselhart, Karl A.

    1999-01-01

    A high-speed civil transport configuration with a Mach number of 1.6 was developed as part of the NASA High-Speed Research Program to serve as a baseline for assessing advanced technologies required for an aircraft with a service entry date of 2005. This configuration offered more favorable solutions to environmental concerns than configurations with higher Mach numbers. The Mach 1.6 configuration was designed for a 6500 n.mi. mission with a 250-passenger payload. The baseline configuration has a wing area of 8732 square feet a takeoff gross weight of 591570 lb, and four 41000-lb advanced turbine bypass engines defined by NASA. These engines have axisymmetric mixer-ejector nozzles that are assumed to yield 20 dB of noise suppression during takeoff, which is assumed to satisfy, the FAR Stage III noise requirements. Any substantial reduction in this assumed level of suppression would require oversizing the engines to meet community noise regulations and would severly impact the gross weight of the aircraft at takeoff. These engines yield a ratio of takeoff thrust to weight of 0.277 and a takeoff wing loading of 67.8 lb/square feet that results in a rotation speed of 169 knots. The approach velocity of the sized configuration at the end of the mission is 131 knots. The baseline configuration was resized with an engine having a projected life of 9000 hr for hot rotating parts and 18000 hr for the rest of the engine, as required for commercial use on an aircraft with a service entry date of 2005. Results show an increase in vehicle takeoff gross weight of approximately 58700 lb. This report presents the details of the configuration development, mass properties, aerodynamic design, propulsion system and integration, mission performance, and sizing.

  1. Renormalized Volume

    Science.gov (United States)

    Gover, A. Rod; Waldron, Andrew

    2017-09-01

    We develop a universal distributional calculus for regulated volumes of metrics that are suitably singular along hypersurfaces. When the hypersurface is a conformal infinity we give simple integrated distribution expressions for the divergences and anomaly of the regulated volume functional valid for any choice of regulator. For closed hypersurfaces or conformally compact geometries, methods from a previously developed boundary calculus for conformally compact manifolds can be applied to give explicit holographic formulæ for the divergences and anomaly expressed as hypersurface integrals over local quantities (the method also extends to non-closed hypersurfaces). The resulting anomaly does not depend on any particular choice of regulator, while the regulator dependence of the divergences is precisely captured by these formulæ. Conformal hypersurface invariants can be studied by demanding that the singular metric obey, smoothly and formally to a suitable order, a Yamabe type problem with boundary data along the conformal infinity. We prove that the volume anomaly for these singular Yamabe solutions is a conformally invariant integral of a local Q-curvature that generalizes the Branson Q-curvature by including data of the embedding. In each dimension this canonically defines a higher dimensional generalization of the Willmore energy/rigid string action. Recently, Graham proved that the first variation of the volume anomaly recovers the density obstructing smooth solutions to this singular Yamabe problem; we give a new proof of this result employing our boundary calculus. Physical applications of our results include studies of quantum corrections to entanglement entropies.

  2. Rice OsGL1-6 is involved in leaf cuticular wax accumulation and drought resistance.

    Directory of Open Access Journals (Sweden)

    Lingyan Zhou

    Full Text Available Cuticular wax is a class of organic compounds that comprises the outermost layer of plant surfaces. Plant cuticular wax, the last barrier of self-defense, plays an important role in plant growth and development. The OsGL1-6 gene, a member of the fatty aldehyde decarbonylase gene family, is highly homologous to Arabidopsis CER1, which is involved in cuticular wax biosynthesis. However, whether OsGL1-6 participates in cuticular wax biosynthesis remains unknown. In this study, an OsGL1-6 antisense-RNA vector driven by its own promoter was constructed and introduced into the rice variety Zhonghua11 by Agrobacterium-mediated transformation to obtain several independent transgenic plants with decreased OsGL1-6 expression. These OsGL1-6 antisense-RNA transgenic plants showed droopy leaves at the booting stage, significantly decreased leaf cuticular wax deposition, thinner cuticle membrane, increased chlorophyll leaching and water loss rates, and enhanced drought sensitivity. The OsGL1-6 gene was constitutively expressed in all examined organs and was very highly expressed in leaf epidermal cells and vascular bundles. The transient expression of OsGL1-6-GFP fusion indicated that OsGL1-6 is localized in the endoplasmic reticulum. Qualitative and quantitative analysis of the wax composition using gas chromatography-mass spectrometry revealed a significantly reduced total cuticular wax load on the leaf blades of the OsGL1-6 antisense-RNA transgenic plants as well as markedly decreased alkane and aldehyde contents. Their primary alcohol contents increased significantly compared with those in the wild type plants, suggesting that OsGL1-6 is associated with the decarbonylation pathways in wax biosynthesis. We propose that OsGL1-6 is involved in the accumulation of leaf cuticular wax and directly impacts drought resistance in rice.

  3. 1/6TH SCALE STRIP EFFLUENT FEED TANK-MIXING RESULTS USING MCU SOLVENT

    Energy Technology Data Exchange (ETDEWEB)

    Hansen, E

    2006-02-01

    The purpose of this task was to determine if mixing was an issue for the entrainment and dispersion of the Modular Caustic Side Solvent Extraction (CSSX) Unit (MCU) solvent in the Defense Waste Processing Facility (DWPF) Strip Effluent Feed Tank (SEFT). The MCU strip effluent stream containing the Cs removed during salt processing will be transferred to the DWPF for immobilization in HLW glass. In lab-scale DWPF chemical process cell testing, mixing of the solvent in the dilute nitric acid solution proved problematic, and the Savannah River National Laboratory (SRNL) was requested to perform scaled SEFT mixing tests to evaluate whether the problem was symptomatic of the lab-scale set-up or of the solvent. The solvent levels tested were 228 and 235 ppm, which represented levels near the estimated DWPF solvent limit of 239 ppm in 0.001M HNO{sub 3} solution. The 239 ppm limit was calculated by Norato in X-CLC-S-00141. The general approach for the mixing investigation was to: (1) Investigate the use of fluorescent dyes to aid in observing the mixing behavior. Evaluate and compare the physical properties of the fluorescent dyed MCU solvents to the baseline Oak Ridge CSSX solvent. Based on the data, use the dyed MCU solvent that best approximates the physical properties. (2) Use approximately a 1/6th linear scale of the SEFT to replicate the internal configuration for DWPF mixing. (3) Determine agitator speed(s) for scaled testing based on the DWPF SEFT mixing speed. (4) Perform mixing tests using the 1/6th SEFT and determine any mixing issues (entrainment/dispersion, accumulation, adhesion) through visual observations and by pulling samples to assess uniformity. The mixing tests used MCU solvent fabricated at SRNL blended with Risk Reactor DFSB-K43 fluorescent dye. This dyed SRNL MCU solvent had equivalent physical properties important to mixing as compared to the Oak Ridge baseline solvent, blended easily with the MCU solvent, and provided an excellent visual aid.

  4. A genetically anchored physical framework for Theobroma cacao cv. Matina 1-6

    Directory of Open Access Journals (Sweden)

    Kuhn David N

    2011-08-01

    Full Text Available Abstract Background The fermented dried seeds of Theobroma cacao (cacao tree are the main ingredient in chocolate. World cocoa production was estimated to be 3 million tons in 2010 with an annual estimated average growth rate of 2.2%. The cacao bean production industry is currently under threat from a rise in fungal diseases including black pod, frosty pod, and witches' broom. In order to address these issues, genome-sequencing efforts have been initiated recently to facilitate identification of genetic markers and genes that could be utilized to accelerate the release of robust T. cacao cultivars. However, problems inherent with assembly and resolution of distal regions of complex eukaryotic genomes, such as gaps, chimeric joins, and unresolvable repeat-induced compressions, have been unavoidably encountered with the sequencing strategies selected. Results Here, we describe the construction of a BAC-based integrated genetic-physical map of the T. cacao cultivar Matina 1-6 which is designed to augment and enhance these sequencing efforts. Three BAC libraries, each comprised of 10× coverage, were constructed and fingerprinted. 230 genetic markers from a high-resolution genetic recombination map and 96 Arabidopsis-derived conserved ortholog set (COS II markers were anchored using pooled overgo hybridization. A dense tile path consisting of 29,383 BACs was selected and end-sequenced. The physical map consists of 154 contigs and 4,268 singletons. Forty-nine contigs are genetically anchored and ordered to chromosomes for a total span of 307.2 Mbp. The unanchored contigs (105 span 67.4 Mbp and therefore the estimated genome size of T. cacao is 374.6 Mbp. A comparative analysis with A. thaliana, V. vinifera, and P. trichocarpa suggests that comparisons of the genome assemblies of these distantly related species could provide insights into genome structure, evolutionary history, conservation of functional sites, and improvements in physical map

  5. A genetically anchored physical framework for Theobroma cacao cv. Matina 1-6.

    Science.gov (United States)

    Saski, Christopher A; Feltus, Frank A; Staton, Margaret E; Blackmon, Barbara P; Ficklin, Stephen P; Kuhn, David N; Schnell, Raymond J; Shapiro, Howard; Motamayor, Juan Carlos

    2011-08-16

    The fermented dried seeds of Theobroma cacao (cacao tree) are the main ingredient in chocolate. World cocoa production was estimated to be 3 million tons in 2010 with an annual estimated average growth rate of 2.2%. The cacao bean production industry is currently under threat from a rise in fungal diseases including black pod, frosty pod, and witches' broom. In order to address these issues, genome-sequencing efforts have been initiated recently to facilitate identification of genetic markers and genes that could be utilized to accelerate the release of robust T. cacao cultivars. However, problems inherent with assembly and resolution of distal regions of complex eukaryotic genomes, such as gaps, chimeric joins, and unresolvable repeat-induced compressions, have been unavoidably encountered with the sequencing strategies selected. Here, we describe the construction of a BAC-based integrated genetic-physical map of the T. cacao cultivar Matina 1-6 which is designed to augment and enhance these sequencing efforts. Three BAC libraries, each comprised of 10× coverage, were constructed and fingerprinted. 230 genetic markers from a high-resolution genetic recombination map and 96 Arabidopsis-derived conserved ortholog set (COS) II markers were anchored using pooled overgo hybridization. A dense tile path consisting of 29,383 BACs was selected and end-sequenced. The physical map consists of 154 contigs and 4,268 singletons. Forty-nine contigs are genetically anchored and ordered to chromosomes for a total span of 307.2 Mbp. The unanchored contigs (105) span 67.4 Mbp and therefore the estimated genome size of T. cacao is 374.6 Mbp. A comparative analysis with A. thaliana, V. vinifera, and P. trichocarpa suggests that comparisons of the genome assemblies of these distantly related species could provide insights into genome structure, evolutionary history, conservation of functional sites, and improvements in physical map assembly. A comparison between the two T. cacao

  6. A Photometrically Detected Forming Cluster of Galaxies at Redshift 1.6 in the GOODS Field

    CERN Document Server

    Castellano, M; Trevese, D; Grazian, A; Pentericci, L; Fiore, F; Fontana, A; Giallongo, E; Santini, P; Cristiani, S; Nonino, M; Vanzella, E

    2007-01-01

    We report the discovery of a localized overdensity at z~1.6 in the GOODS-South Field, presumably a poor cluster in the process of formation. The three-dimensional galaxy density has been estimated on the basis of well calibrated photometric redshifts from the multiband photometric GOODS-MUSIC catalog using the (2+1)D technique. The density peak is embedded in the larger scale overdensity of galaxies known to exist at z=1.61 in the area. The properties of the member galaxies are compared to those of the surrounding field and we found that the two populations are significantly different supporting the reality of the structure. The reddest galaxies, once evolved according to their best fit models, have colors consistent with the red sequence of lower redshift clusters. The estimated M_200 total mass of the cluster is in the range 1.3 x 10^14 - 5.7x 10^14 Msun, depending on the assumed bias factor b. An upper limit for the 2-10 keV X-ray luminosity, based on the 1Ms Chandra observations, is L_X=0.5 x 10^43 erg s^...

  7. Microwave Emission of Supra-arcade Structure associated with M1.6 Limb Flare

    CERN Document Server

    Kim, Sujin; Shibasaki, Kiyoto; Reznikova, Veronika

    2012-01-01

    We have investigated a supra-arcade structure, associated with an M1.6 flare, which occurred on the south-east limb on 4th of November 2010. It is observed in microwaves at 17 GHz with the Nobeyama Radioheliograph (NoRH), soft X-rays in the range of 8-20 keV with the Reuven Ramaty High Energy Solar Spectroscopic Imager (RHESSI), and EUV with the Atmospheric Imaging Assembly (AIA) onboard the Solar Dynamics Observatory (SDO). As reported by Reeves & Golub (2011), the supra-arcade structure is observed predominantly in the AIA 131 {\\AA} channel, which contains a hot 11 MK component from Fe XIX (Boerner et al. 2011). While this hot flare plasma lasts over the decay phase of the flare, it shows some interesting characteristics in microwaves and soft X-rays: 1) In the supra-arcade structure, the brightness temperature (TB) of the microwave emission increases gradually up to 2\\times10^4 K, and 2) two soft X-ray sources appear: one cospatial with the supra-arcade structure and another above the post-flare arcade...

  8. Di-Isocyanate Crosslinked Aerogels with 1, 6-Bis (Trimethoxysilyl) Hexane Incorporated in Silica Backbone

    Science.gov (United States)

    Vivod, Stephanie L.; Meador, Mary Ann B.; Nguyen, Baochau N.; Quade, Derek; Randall, Jason; Perry, Renee

    2008-01-01

    Silica aerogels are desirable materials for many applications that take advantage of their light weight and low thermal conductivity. Addition of a conformal polymer coating which bonds with the amine decorated surface of the silica network improves the strength of the aerogels by as much as 200 times. Even with vast improvement in strength they still tend to undergo brittle failure due to the rigid silica backbone. We hope to increase the flexibility and elastic recovery of the silica based aerogel by altering the silica back-bone by incorporation of more flexible hexane links. To this end, we investigated the use of 1,6-bis(trimethoxysilyl)hexane (BTMSH), a polysilsesquioxane precursor3, as an additional co-reactant to prepare silica gels which were subsequently cross-linked with di-isocyanate. Previously, this approach of adding flexibility by BTMSH incorporation was demonstrated with styrene cross-linked aerogels. In our study, we varied silane concentration, mol % of silicon from BTMSH and di-isocyanate concentration by weight percent to attempt to optimize both the flexibility and the strength of the aerogels.

  9. Osteoblast biocompatibility of pre-mineralized, hexamethylene-1,6-diaminocarboxysulphonate crosslinked chitosan fibers

    Science.gov (United States)

    Kiechel, Marjorie A.; Beringer, Laura T.; Donius, Amalie E.; Komiya, Yuko; Habas, Raymond; Wegst, Ulrike G. K.; Schauer, Caroline L.

    2015-01-01

    Biopolymer-ceramic composites are thought to be particularly promising materials for bone tissue engineering as they more closely mimic natural bone. Here, we demonstrate the fabrication by electrospinning of fibrous chitosan-hydroxyapatite composite scaffolds with low (1 wt%) and high (10 wt%) mineral contents. Scanning electron microscopy (FESEM), energy dispersive spectroscopy (EDS) and unidirectional tensile testing were performed to determine fiber surface morphology, elemental composition, and tensile Young’s modulus (E) and ultimate tensile strength (σUTS), respectively. EDS scans of the scaffolds indicated that the fibers, crosslinked with either hexamethylene-1,6-diaminocarboxysulfonate (HDACS) or genipin, have a crystalline hydroxyapatite mineral content at 10 wt% additive. Moreover, FESEM micrographs showed that all electrospun fibers have diameters (122 – 249 nm), which fall within the range of those of fibrous collagen found in the extracellular matrix of bone. Young’s modulus and ultimate tensile strength of the various crosslinked composite compositions were in the range of 116 – 329 MPa and 2 – 15 MPa, respectively. Osteocytes seeded onto the mineralized fibers were able to demonstrate good biocompatibility enhancing the potential use for this material in future bone tissue engineering applications. PMID:25771925

  10. Osteoblast biocompatibility of premineralized, hexamethylene-1,6-diaminocarboxysulfonate crosslinked chitosan fibers.

    Science.gov (United States)

    Kiechel, Marjorie A; Beringer, Laura T; Donius, Amalie E; Komiya, Yuko; Habas, Raymond; Wegst, Ulrike G K; Schauer, Caroline L

    2015-10-01

    Biopolymer-ceramic composites are thought to be particularly promising materials for bone tissue engineering as they more closely mimic natural bone. Here, we demonstrate the fabrication by electrospinning of fibrous chitosan-hydroxyapatite composite scaffolds with low (1 wt %) and high (10 wt %) mineral contents. Scanning electron microscopy (FESEM), energy dispersive spectroscopy (EDS) and unidirectional tensile testing were performed to determine fiber surface morphology, elemental composition, and tensile Young's modulus (E) and ultimate tensile strength (σUTS ), respectively. EDS scans of the scaffolds indicated that the fibers, crosslinked with either hexamethylene-1,6-diaminocarboxysulfonate (HDACS) or genipin, have a crystalline hydroxyapatite mineral content at 10 wt % additive. Moreover, FESEM micrographs showed that all electrospun fibers have diameters (122-249 nm), which fall within the range of those of fibrous collagen found in the extracellular matrix of bone. Young's modulus and ultimate tensile strength of the various crosslinked composite compositions were in the range of 116-329 MPa and 2-15 MPa, respectively. Osteocytes seeded onto the mineralized fibers were able to demonstrate good biocompatibility enhancing the potential use for this material in future bone tissue engineering applications.

  11. Fructose-1,6-bisphosphatase mediates cellular responses to DNA damage and aging in Saccharomyces cerevisiae

    Energy Technology Data Exchange (ETDEWEB)

    Kitanovic, Ana [Institut fuer Pharmazie und Molekulare Biotechnologie, Ruprecht-Karls-Universitaet Heidelberg, Im Neuenheimer Feld 364, D-69120 Heidelberg (Germany); Woelfl, Stefan [Institut fuer Pharmazie und Molekulare Biotechnologie, Ruprecht-Karls-Universitaet Heidelberg, Im Neuenheimer Feld 364, D-69120 Heidelberg (Germany)]. E-mail: wolfl@uni-hd.de

    2006-02-22

    Response to DNA damage, lack of nutrients and other stress conditions is an essential property of living systems. The coordinate response includes DNA damage repair, activation of alternate biochemical pathways, adjustment of cellular proliferation and cell cycle progression as well as drastic measures like cellular suicide which prevents proliferation of severely damaged cells. Investigating the transcriptional response of Saccharomyces cerevisiae to low doses of the alkylating agent methylmethane sulfonate (MMS) we observed induction of genes involved in glucose metabolism. RT-PCR analysis showed that the expression of the key enzyme in gluconeogenesis fructose-1,6-bisphosphatase (FBP1) was clearly up-regulated by MMS in glucose-rich medium. Interestingly, deletion of FBP1 led to reduced sensitivity to MMS, but not to other DNA-damaging agents, such as 4-NQO or phleomycin. Reintroduction of FBP1 in the knockout restored the wild-type phenotype while overexpression increased MMS sensitivity of wild-type, shortened life span and increased induction of RNR2 after treatment with MMS. Deletion of FBP1 reduced production of reactive oxygen species (ROS) in response to MMS treatment and in untreated aged cells, and increased the amount of cells able to propagate and to form colonies, but had no influence on the genotoxic effect of MMS. Our results indicate that FBP1 influences the connection between DNA damage, aging and oxidative stress through either direct signalling or an intricate adaptation in energy metabolism.0.

  12. The 1.6 Å resolution structure of a FRET-optimized Cerulean fluorescent protein

    Energy Technology Data Exchange (ETDEWEB)

    Watkins, Jennifer L.; Kim, Hanseong [Arizona State University, Tempe, AZ 85287-1604 (United States); Markwardt, Michele L. [University of Maryland School of Medicine, Baltimore, MD 21201-1559 (United States); Chen, Liqing; Fromme, Raimund [Arizona State University, Tempe, AZ 85287-1604 (United States); Rizzo, Mark A. [University of Maryland School of Medicine, Baltimore, MD 21201-1559 (United States); Wachter, Rebekka M., E-mail: rwachter@asu.edu [Arizona State University, Tempe, AZ 85287-1604 (United States)

    2013-05-01

    The high resolution X-ray structure of the cyan fluorescent protein mCerulean3 demonstrates that different combinations of correlated residue substitutions can provide near optimum quantum yield values for fluorescence. Genetically encoded cyan fluorescent proteins (CFPs) bearing a tryptophan-derived chromophore are commonly used as energy-donor probes in Förster resonance energy transfer (FRET) experiments useful in live cell-imaging applications. In recent years, significant effort has been expended on eliminating the structural and excited-state heterogeneity of these proteins, which has been linked to undesirable photophysical properties. Recently, mCerulean3, a descendant of enhanced CFP, was introduced as an optimized FRET donor protein with a superior quantum yield of 0.87. Here, the 1.6 Å resolution X-ray structure of mCerulean3 is reported. The chromophore is shown to adopt a planar trans configuration at low pH values, indicating that the acid-induced isomerization of Cerulean has been eliminated. β-Strand 7 appears to be well ordered in a single conformation, indicating a loss of conformational heterogeneity in the vicinity of the chromophore. Although the side chains of Ile146 and Leu167 appear to exist in two rotamer states, they are found to be well packed against the indole group of the chromophore. The Ser65 reversion mutation allows improved side-chain packing of Leu220. A structural comparison with mTurquoise2 is presented and additional engineering strategies are discussed.

  13. Cloning and expression analysis of the chloroplast fructose-1,6-bisphosphatase gene from Pyropia haitanensis

    Institute of Scientific and Technical Information of China (English)

    XIAO Haidong; CHEN Changsheng; XU Yan; JI Dehua; XIE Chaotian

    2014-01-01

    Fructose-1,6-bisphosphatase (FBPase) is one of the key enzymes in Calvin circle and starch biosynthesis. In this study, the full-length of cpFBPase gene from Pyropia haitanensis was cloned by using rapid amplifica-tion of cDNA ends (RACE) technology. The nucleotide sequence of PhcpFBPase consists of 1 400 bp, includ-ing a 5′untranslated region (UTR) of 92 bp, a 3′ UTR of 69 bp, and an open reading frame (ORF) of 1 236 bp, which can be translated into a 412-amino-acid putative peptides with a molecular weight of 44.3 kDa and a theoretical pI of 5.23. Multiple sequence alignment indicated that the protein belonged to the chloroplast FBPase enzyme. Phylogenetic analysis showed that the protein assembled with the cpFBPase of a thermal tolerant unicellular red micro-algae Galdieria sulphuraria. Expression patterns analyzed by qRT-PCR re-vealed that the expression of PhcpFBPase gene in the thallus phage was 7-fold higher than in the conchocelis phage, which suggested the different mechanisms of inorganic carbon utilization among the different life phages of P. haitanensis. And the different response modes of PhcpFBPase mRNA levels to high temperature and desiccation stress indicated that PhcpFBPase played an important role in responsing to abiotic stress.

  14. Orbital responses to methyl sites in CnH2n+2 (n=1-6)

    Institute of Scientific and Technical Information of China (English)

    Yang Ze-Jin; Cheng Xin-Lu; Zhu Zheng-He; Yang Xiang-Dong

    2012-01-01

    Orbital responses to methyl sites in CnH2n+2 (n =1-6) are studied by B3LYP/TZVP based on the most stable geometries using the B3LYP/aug-cc-pVTZ method.Vertical ionization energies are produced using the SAOP/et-pVQZ model for the complete valence space.The highest occupied molecular orbital (HOMO) investigations indicate the pelectron profiles in methane,ethane,propane,and n-butane.By increasing the number of carbon-carbon bonds in lower momentum regions,the s,p-hybridized orbitals are built and display strong exchange and correlation interactions in lower momentum space (P (≤) 0.50 a.u.).Meanwhile,the relative intensities of the isomers in lower momentum space show the strong bonding number dependence of the carbon-carbon bonds,meaning that more electrons have contributed to orbital construction.The study of representative valence orbital momentum distribution further confirms that the structural changes lead to evident electronic rearrangement over the whole valence space.An analysis based on the isomers reveals that the valence orbitals are isomer-dependent and the valence ionization energy experiences an apparent shift in the inner valence space.However,such shifts are greatly reduced in the outer valence space.Meanwhile,the opposite energy shift trend is found in the intermediate valence space.

  15. Inferno: June 1943 to May 1945. Book 5. Holocaust Series, First Edition.

    Science.gov (United States)

    Ayer, Eleanor H.

    There are eight volumes in this series on the Holocaust. Volumes 1-6 recount the history of the Jewish people in various year groupings: (1) "Ancient 1935"; (2) "September 1935 to December 1938"; (3) "January 1939 to December 1941"; (4) "January 1942 to June 1943"; (5) "July 1943 to April 1945";…

  16. Inferno: June 1943 to May 1945. Book 5. Holocaust Series, First Edition.

    Science.gov (United States)

    Ayer, Eleanor H.

    There are eight volumes in this series on the Holocaust. Volumes 1-6 recount the history of the Jewish people in various year groupings: (1) "Ancient 1935"; (2) "September 1935 to December 1938"; (3) "January 1939 to December 1941"; (4) "January 1942 to June 1943"; (5) "July 1943 to April 1945";…

  17. Food consumption and nutrient intake in Finnish 1-6-year-old children.

    Science.gov (United States)

    Kyttälä, Pipsa; Erkkola, Maijaliisa; Kronberg-Kippilä, Carina; Tapanainen, Heli; Veijola, Riitta; Simell, Olli; Knip, Mikael; Virtanen, Suvi M

    2010-06-01

    To study food consumption and nutrient intake in Finnish children aged 1-6 years and to assess the effect of age and sex on food consumption and nutrient intake. Cross-sectional samples of children participating in the Type 1 Diabetes Prediction and Prevention (DIPP) birth cohort study in Finland. The study population comprised healthy children recruited in the nutrition study within the DIPP study in 1998-2003. Three-day food records (2535 in total) from 1-, 2-, 3-, 4- and 6-year-old children were kept between the years 2003 and 2005. The energy-adjusted consumption of fruits and berries, cereal products, infant formulas and meat dishes was higher and the consumption of vegetables, salads, breads, dairy products, fat spreads, drinks, sweets and sugar was lower among 1-year-old children than older age groups (P for all <0.05). The mean daily energy intake increased with age and was higher among boys than girls in all age groups, except among the 2-year-olds (P for all <0.05). The diet of the 2-6-year-old children contained too much saturated fat and sucrose, and too little PUFA compared with the current Nordic Nutrition Recommendations. The intakes of most vitamins and minerals met the recommendations. However, the intakes of vitamin D, E and iron fell below the recommended levels. The nutrient density of the diet decreased after the age of 1 year at the time that the children adapted to the regular family diet. In order to improve the diet of young children, it is essential to evaluate the diet of the whole family.

  18. Experimental results from pressure testing a 1:6-scale nuclear power plant containment

    Energy Technology Data Exchange (ETDEWEB)

    Horschel, D.S. [Sandia National Labs., Albuquerque, NM (United States)

    1992-01-01

    This report discusses the testing of a 1:6-scale, reinforced-concrete containment building at Sandia National Laboratories, in Albuquerque, New Mexico. The scale-model, Light Water Reactor (LWR) containment building was designed and built to the American Society of Mechanical Engineers (ASME) code by United Engineers and Constructors, Inc., and was instrumented with over 1200 transducers to prepare for the test. The containment model was tested to failure to determine its response to static internal overpressurization. As part of the US Nuclear Regulatory Commission`s program on containment integrity, the test results will be used to assess the capability of analytical methods to predict the performance of containments under severe-accident loads. The scaled dimensions of the cylindrical wall and hemispherical dome were typical of a full-size containment. Other typical features included in the heavily reinforced model were equipment hatches, personnel air locks, several small piping penetrations, and a ihin steel liner that was attached to the concrete by headed studs. In addition to the transducers attached to the model, an acoustic detection system and several video and still cameras were used during testing to gather data and to aid in the conduct of the test. The model and its instrumentation are briefly discussed, and is followed by the testing procedures and measured response of the containment model. A summary discussion is included to aid in understanding the significance of the test as it applies to real world reinforced concrete containment structures. The data gathered during SIT and overpressure testing are included as an appendix.

  19. Fructose 1,6-Bisphosphate Aldolase, a Novel Immunogenic Surface Protein on Listeria Species

    Science.gov (United States)

    Conceição, Fabricio Rochedo; Hust, Michael; Mendonça, Karla Sequeira; Moreira, Ângela Nunes; França, Rodrigo Correa; da Silva, Wladimir Padilha; Aleixo, José Antonio G.

    2016-01-01

    Listeria monocytogenes is a ubiquitous food-borne pathogen, and its presence in food or production facilities highlights the importance of surveillance. Increased understanding of the surface exposed antigens on Listeria would provide potential diagnostic and therapeutic targets. In the present work, using mass spectrometry and genetic cloning, we show that fructose-1,6-bisphosphate aldolase (FBA) class II in Listeria species is the antigen target of the previously described mAb-3F8. Western and dot blot assays confirmed that the mAb-3F8 could distinguish all tested Listeria species from close-related bacteria. Localization studies indicated that FBA is present in every fraction of Listeria cells, including supernatant and the cell wall, setting Listeria spp. as one of the few bacteria described to have this protein on their cell surface. Epitope mapping using ORFeome display and a peptide membrane revealed a 14-amino acid peptide as the potential mAb-3F8 epitope. The target epitope in FBA allowed distinguishing Listeria spp. from closely-related bacteria, and was identified as part of the active site in the dimeric enzyme. However, its function in cell surface seems not to be host cell adhesion-related. Western and dot blot assays further demonstrated that mAb-3F8 together with anti-InlA mAb-2D12 could differentiate pathogenic from non-pathogenic Listeria isolated from artificially contaminated cheese. In summary, we report FBA as a novel immunogenic surface target useful for the detection of Listeria genus. PMID:27489951

  20. Irradiation damage induced on polyethylene terephtalate by 1.6 MeV deuteron ions

    Energy Technology Data Exchange (ETDEWEB)

    Abdesselam, M. [USTHB, Faculte de Physique, B.P. 32, El Alia, 16111 BEZ Algiers (Algeria); Djebara, M. [USTHB, Faculte de Physique, B.P. 32, El Alia, 16111 BEZ Algiers (Algeria)], E-mail: matjbara@yahoo.com; Chami, A.C. [USTHB, Faculte de Physique, B.P. 32, El Alia, 16111 BEZ Algiers (Algeria); Siad, M. [DPN, CNRA, 02 Bd Frantz Fanon, B.P. 399, Alger-gare (Algeria)

    2008-09-15

    The irradiation damage caused on polyethylene terephtalate (Mylar, PET) samples by 1.6 MeV deuteron ions has been measured using simultaneously the nuclear reaction analysis (NRA) and the transmission energy loss (TEL) techniques. The irradiation was carried out at normal incidence relative to the target surface with the irradiation beam being used as the analysis beam. The evolution of the overall damage during irradiation was evaluated by measuring the variation of the energy loss of the deuteron beam passing through the target. For this purpose, a solid state Si detector placed at a forward angle of 30 deg. relative to the incident beam direction was used. The NRA spectra recorded by a second Si detector located backward at 150 deg. allowed the evaluation of the carbon and the oxygen depletion. The beam spot size was circular in shape and 1 mm in diameter and the beam current was set at 5 nA. The ion fluence was increased up to the value of 2.5 x 10{sup 16} deuterons/cm{sup 2}. It was observed that the target energy loss decreased steadily as the fluence increased and levelled off at high fluence. The {sup 16}O(d,p{sub 0}){sup 17}O, {sup 16}O(d,p{sub 1}){sup 17}O{sup *} and {sup 12}C(d,p{sub 0}){sup 13}C reactions were used for monitoring the evolution of the oxygen and carbon content as a function of the deuteron fluence. A monotonic decrease of the oxygen content with the increase of ion fluence was observed. At the highest fluence the oxygen depletion reached a value of about 75%. For carbon, a weak depletion was observed at fluence ranging from 2.5 x 10{sup 15} d/cm{sup 2} to 1.0 x 10{sup 16} d/cm{sup 2} followed by a levelling-off with a total loss around 20%.

  1. A Recount of Sunspot Groups on Staudach's Drawings

    Science.gov (United States)

    Svalgaard, Leif

    2017-01-01

    We have examined the more than 1100 drawings of the solar disk made by the German amateur astronomer Johann Caspar Staudach during 1749 - 1799 and counted the spots on each image. Using the modern perception of how to group spots into active regions, we regrouped the spots as a modern observer would. The resulting number of groups was found to be higher on average by 25 % than the first count of groups performed by Wolf in 1857, which was used by Hoyt and Schatten ( Solar Phys. 181, 491, 1998) in their construction of the group sunspot number. Compared to other observers at the time, Staudach's drawings have a very low average number, about two, of spots per group, possibly indicating an inferior telescope that probably suffered from spherical and chromatic aberration, as would be typical of amateur telescopes of the day. We have initiated an ongoing project aiming at observing sunspots with antique telescopes having similar defects in order to determine the factor necessary to bring the Staudach observations onto a modern scale.

  2. A Recount of Sunspot Groups on Staudach's Drawings

    CERN Document Server

    Svalgaard, Leif

    2015-01-01

    We have examined the more than 1100 drawings of the solar disk made by the German astronomy amateur Johann Caspar Staudach during 1749-1799 and counted the spots on each image. Using the modern perception of how to group spots into active regions we regrouped the spots as a modern observer would. The resulting number of groups was found to be on average 25% higher than the first count of groups performed by Wolf in 1857, and used by Hoyt and Schatten in their construction of the Group Sunspot Number. Compared to other observers at the time, Staudach's drawings have a very low average number, ~2, of spots per group, possibly indicating an inferior telescope likely suffering from spherical and chromatic aberration as would typical of amateur telescopes of the day. We have initiated an ongoing project aiming at observing sunspots with antique telescopes having similar defects in order to determine the factor necessary to bring the Staudach observations onto a modern scale.

  3. Human fructose-1,6-bisphosphatase gene (FBP1): Exon-intron organization, localization to chromosome bands 9q22.2-q22.3, and mutation screening in subjects with fructose-1,6-bisphosphatase deficiency

    Energy Technology Data Exchange (ETDEWEB)

    El-Maghrabi, M.R.; Jiang, W. [State Univ. of New York, Stony Brook, NY (United States)] [and others

    1995-06-10

    Fructose-1,6-bisphosphatase (EC 3.1.3.11) is a key regulatory enzyme of gluconeogenesis that catalyzes the hydrolysis of fructose-1,6-bisphosphate to generate fructose-6-phosphate and inorganic phosphate. Deficiency of fructose-1,6-bisphosphatase is associated with fasting hypoglycemia and metabolic acidosis because of impaired gluconeogenesis. We have cloned and characterized the human liver fructose-1,6-bisphosphatase gene (FBP1). FBP1, localized to chromosome bands 9q22.2-q22.3 by fluorescence in situ hybridization, consists of seven exons that span > 31 kb, and the six introns are in the same position as in the rat gene. FBP1 was screened for mutations in two subjects with fructose-1,6-bisphosphatase deficiency. Four nucleotide substitutions were identified, two of which were silent mutations in the codons for Ala-216 (GCT {yields} GCC) and Gly-319 (GGG {yields} GGA). The other substitutions were in intron 3, a C {yields} T substitution 7 nucleotides downstream from the splice donor site, and in the promoter region, an A {yields} T substitution 188 nucleotides upstream from the start of transcription. These nucleotide substitutions were also found in normal unaffected subjects and thus are not the cause of fructose-1,6-bisphosphatase deficiency in the two subjects studied. The molecular basis of hepatic fructose-1,6-bisphosphatase deficiency in these subjects remains undetermined but could result from unidentified mutations in the promoter that decrease expression or from mutations in another gene that indirectly lead to decreased fructose-1,6-bisphosphatase activity. 18 refs., 3 figs., 3 tabs.

  4. The crystal structure of 1,6-dithiapyrene(DTP)-7,7,8,8-tetracyano-p-quinodimethane(TCNQ)

    DEFF Research Database (Denmark)

    Thorup, Niels; Rindorf, G.; Jacobsen, C. S.;

    1985-01-01

    The crystal structure of the charge-transfer complex between 1,6-dithiapyrene (DTP) and 7,7,8,8-tetracyano-p-quino-dimethane (TCNQ) is reported. The structure correlates well with the observed physical properties......The crystal structure of the charge-transfer complex between 1,6-dithiapyrene (DTP) and 7,7,8,8-tetracyano-p-quino-dimethane (TCNQ) is reported. The structure correlates well with the observed physical properties...

  5. The crystal structure of 1,6-dithiapyrene(DTP)-7,7,8,8-tetracyano-p-quinodimethane(TCNQ)

    DEFF Research Database (Denmark)

    Thorup, Niels; Rindorf, G.; Jacobsen, C. S.

    1985-01-01

    The crystal structure of the charge-transfer complex between 1,6-dithiapyrene (DTP) and 7,7,8,8-tetracyano-p-quino-dimethane (TCNQ) is reported. The structure correlates well with the observed physical properties......The crystal structure of the charge-transfer complex between 1,6-dithiapyrene (DTP) and 7,7,8,8-tetracyano-p-quino-dimethane (TCNQ) is reported. The structure correlates well with the observed physical properties...

  6. Analysis of the site-specific integration system of the Streptomyces aureofaciens phage μ1/6.

    Science.gov (United States)

    Farkašovská, Jarmila; Godány, Andrej

    2012-03-01

    The bacteriophage μ1/6 integrates its DNA into the chromosome of tetracycline producing strains of Streptomyces aureofaciens by a site-specific recombination process. A bioinformatic analysis of the μ1/6 genome revealed that orf5 encodes a putative integrase, a basic protein of 416 amino acids. The μ1/6 integrase was found to belong to the integrase family of site-specific tyrosine recombinases. The phage attachment site (attP) was localized downstream of the int gene. The attachment junctions (attL and attR) were determined, allowing identification of the bacterial attachment site (attB). All attachment sites shared a 46-bp common core sequence within which a site-specific recombination occurs. This core sequence comprises the 3' end of a putative tRNA(Thr) gene (anticodon TGT) which is completely restored in attL after integration of the phage into the host genome. An integration vector containing μ1/6 int-attP region was inserted stably into the S. aureofaciens B96, S. lividans TK24, and S. coelicolor A3. The μ1/6 integrase was shown to be functional in vivo in heterologous Escherichia coli without any other factors encoded by Streptomyces. In vitro recombination assay using purified μ1/6 integrase demonstrated its ability to catalyze integrative recombination in the presence of a crude extract of E. coli cells.

  7. Altered Hepa1-6 cells by dimethyl sulfoxide (DMSO)-treatment induce anti-tumor immunity in vivo.

    Science.gov (United States)

    Jiang, Zhengyu; Zhang, Hongxia; Wang, Ye; Yu, Bin; Wang, Chen; Liu, Changcheng; Lu, Juan; Chen, Fei; Wang, Minjun; Yu, Xinlu; Lin, Jiahao; Pan, Xinghua; Wang, Pin; Zhu, Haiying

    2016-02-23

    Cancer immunotherapy is the use of the immune system to treat cancer. Our current research proposed an optional strategy of activating immune system involving in cancer immunotherapy. When being treated with 2% DMSO in culture medium, Hepa1-6 cells showed depressed proliferation with no significant apoptosis or decreased viability. D-hep cells, Hepa1-6 cells treated with DMSO for 7 days, could restore to the higher proliferation rate in DMSO-free medium, but alteration of gene expression profile was irreversible. Interestingly, tumors from D-hep cells, not Hepa1-6 cells, regressed in wild-type C57BL/6 mice whereas D-hep cells exhibited similar tumorigenesis as Hep1-6 cells in immunodeficient mice. As expected, additional Hepa1-6 cells failed to form tumors in the D-hep-C57 mice in which D-hep cells were eliminated. Further research confirmed that D-hep-C57 mice established anti-tumor immunity against Hepa1-6 cells. Our research proposed viable tumor cells with altered biological features by DMSO-treatment could induce anti-tumor immunity in vivo.

  8. Amyloid precursor protein modulates Nav1.6 sodium channel currents through a Go-coupled JNK pathway

    Science.gov (United States)

    Li, Shao; Wang, Xi; Ma, Quan-Hong; Yang, Wu-lin; Zhang, Xiao-Gang; Dawe, Gavin S.; Xiao, Zhi-Cheng

    2016-01-01

    Amyloid precursor protein (APP), commonly associated with Alzheimer’s disease, also marks axonal degeneration. In the recent studies, we demonstrated that APP aggregated at nodes of Ranvier (NORs) in myelinated central nervous system (CNS) axons and interacted with Nav1.6. However, the physiological function of APP remains unknown. In this study, we described reduced sodium current densities in APP knockout hippocampal neurons. Coexpression of APP or its intracellular domains containing a VTPEER motif with Nav1.6 sodium channels in Xenopus oocytes resulted in an increase in peak sodium currents, which was enhanced by constitutively active Go mutant and blocked by a dominant negative mutant. JNK and CDK5 inhibitor attenuated increases in Nav1.6 sodium currents induced by overexpression of APP. Nav1.6 sodium currents were increased by APPT668E (mutant Thr to Glu) and decreased by T668A (mutant Thr to ALa) mutant, respectively. The cell surface expression of Nav1.6 sodium channels in the white matter of spinal cord and the spinal conduction velocity is decreased in APP, p35 and JNK3 knockout mice. Therefore, APP modulates Nav1.6 sodium channels through a Go-coupled JNK pathway, which is dependent on phosphorylation of APP at Thr668. PMID:28008944

  9. Elevated levels of serum antibodies against alpha-1, 6-glucan in patients with systemic lupus erythematosus or rheumatoid arthritis

    Institute of Scientific and Technical Information of China (English)

    Hui Dai; Xiao-Ming Gao

    2011-01-01

    This study was undertaken to investigate whether levels of anti-alpha-1,6-glucan antibodies in human sera correlate with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE).Serum samples were collected from patients with SLE (n =30),RA (n =30) and healthy adult volunteers.IgG,IgA and IgM levels against alpha-1,6-glucan were measured using enzyme linked immunosorbent assays.Anti-alpha-1,6-glucan IgG prevalence was raised in patients with active SLE (73.3%)and RA (60%) compared with healthy controls (13.3%).Strong correlation between anti-alpha-1,6-glucan-IgG levels and anti-perinuclear factor (r =0.642; p< 0.05) in RA patients or anti-nuclear antibodies (r =0.675; p < 0.05)in SLE patients was observed.No significant differences in anti-alpha-1,6-glucan-lgA or-lgM levels were noted between different groups.We conclude that anti-alpha1,6-glucan-lgG levels were significantly elevated in patients with SLE or RA and positively correlated with disease activity.

  10. 1,6-二磷酸果糖治疗小儿病毒性心肌炎临床疗效观察%Clinical effect of Fructose-1, 6-diphosphate on pedo-viral myocarditis

    Institute of Scientific and Technical Information of China (English)

    郭妍妍

    2010-01-01

    目的 观察1,6-二磷酸果糖治疗小儿病毒性心肌炎的临床效果.方法 将122例病毒性心肌炎的患儿随机抽签分治疗组60例及对照组62例,治疗组应用1,6-二磷酸果糖,对照组应用能量合剂.结果 治疗组总有效52例(86.7%),优于对照组42例(67.7%);治疗组合并早搏的患儿减少早搏总有效16例(88.9%),优于对照组19例(70.4%),2组差异有统计学意义(P<0.05).结论 1,6-二磷酸果糖治疗小儿病毒性心肌炎有明显疗效,值得临床应用.%Objectives To investigate the curative effect of Fructose-1, 6-diphosphate on pedo-viral myo-carditis. Methods Sixty patients in the treatment group had Fructose-1, 6-diphosphate and conventional therapy.62 patients in the control group were only treated with conventional therapy. Results The total effective rate of treatment group(86.7%) was higher than that of control group(67.7%). Fructose-1,6-diphosphate increased the effective rate, decreased the myo-creatase and improved the clinical symptoms. Conclusions Fructose-1, 6-diphosphate is effective for treating pedo-viral myocarditis.

  11. Development of a coal-fueled Internal Manifold Heat Exchanger (IMHEX{reg_sign}) molten carbonate fuel cell. Volumes 1--6, Final report

    Energy Technology Data Exchange (ETDEWEB)

    1991-09-01

    The design of a CGMCFC electric generation plant that will provide a cost of eletricity (COE) which is lower than that of current electric generation technologies and which is competitive with other long-range electric generating systems is presented. This effort is based upon the Internal Manifold Heat Exchanger (IMHEX) technology as developed by the Institute of Gas Technology (IGT). The project was executed by selecting economic and performance objectives for alternative plant arrangements while considering process constraints identified during IMHEX fuel cell development activities at ICT. The four major subsystems of a coal-based MCFC power plant are coal gasification, gas purification, fuel cell power generation and the bottoming cycle. The design and method of operation of each subsystem can be varied, and, depending upon design choices, can have major impact on both the design of other subsystems and the resulting cost of electricity. The challenge of this project was to select, from a range of design parameters, those operating conditions that result in a preferred plant design. Computer modelling was thus used to perform sensitivity analyses of as many system variables as program resources and schedules would permit. In any systems analysis, it is imperative that the evaluation methodology be verifiable and comparable. The TAG Class I develops comparable (if imprecise) data on performance and costs for the alternative cases being studied. It identifies, from a range of options, those which merit more exacting scrutiny to be undertaken at the second level, TAG class II analysis.

  12. Renormalized Volume

    CERN Document Server

    Gover, A Rod

    2016-01-01

    For any conformally compact manifold with hypersurface boundary we define a canonical renormalized volume functional and compute an explicit, holographic formula for the corresponding anomaly. For the special case of asymptotically Einstein manifolds, our method recovers the known results. The anomaly does not depend on any particular choice of regulator, but the coefficients of divergences do. We give explicit formulae for these divergences valid for any choice of regulating hypersurface; these should be relevant to recent studies of quantum corrections to entanglement entropies. The anomaly is expressed as a conformally invariant integral of a local Q-curvature that generalizes the Branson Q-curvature by including data of the embedding. In each dimension this canonically defines a higher dimensional generalization of the Willmore energy/rigid string action. We show that the variation of these energy functionals is exactly the obstruction to solving a singular Yamabe type problem with boundary data along the...

  13. Hydrogen bonds and van der Waals forces as tools for the construction of a herringbone pattern in the crystal structure of hexane-1,6-diaminium hexane-1,6-diyl bis-(hydrogen phospho-nate).

    Science.gov (United States)

    Reiss, Guido J; van Megen, Martin; Frank, Walter

    2017-01-01

    The asymmetric unit of the title salt, [H3N(CH2)6NH3][(HO)O2P(CH2)6PO2(OH)], consists of one half of a hexane-1,6-diaminium dication and one half of a hexane-1,6-diyl bis-(hydrogen phospho-nate) dianion. Both are located around different centres of inversion (Wyckoff sites: 2a and 2d) of the space group P21/c. The shape of the hexane-1,6-diaminium cation is best described as a double hook. Both aminium groups as well as the two attached CH2 groups are turned out from the plane of the central four C atoms. In contrast, all six C atoms of the dianion are almost in a plane. The hydrogen phospho-nate (-PO3H) groups of the anions and the aminium groups of the cations form two-dimensional O-H⋯ and O-H⋯N hydrogen-bonded networks parallel to the ac plane, built up from ten-membered and twelve-membered ring motifs with graph-set descriptors R3(3)(10) and R5(4)(12), respectively. These networks are linked by the alkyl-ene chains of the anions and cations. The resulting three-dimensional network shows a herringbone pattern, which resembles the parent structures 1,6-di-amino-hexane and hexane-1,6-di-phospho-nic acid.

  14. 由啤酒废酵母发酵提取1,6-二磷酸果糖的研究%Study on Preparation and Purification of Fructose-1, 6-Diphosphate with Brewer's Yeast

    Institute of Scientific and Technical Information of China (English)

    朱玉强

    2006-01-01

    探讨了由啤酒废酵母发酵提取1,6-二磷酸果糖(FDP)的工艺.正交试验结果表明,发酵液中氯化镁的浓度、酸度是1,6-二磷酸果糖生产最主要的影响因素.最佳发酵参数为:发酵温度37℃,葡萄糖浓度为0.5mol/L,磷酸盐浓度0.4mol/L,酸度6.5,有机溶剂的加量约6%.1,6-二磷酸果糖在发酵液中的得率最高可达69.8mg/mL.

  15. Verification of the sputter-generated 32SFn- (n = 1-6) anions by accelerator mass spectrometry

    Science.gov (United States)

    Mane, R. G.; Surendran, P.; Kumar, Sanjay; Nair, J. P.; Yadav, M. L.; Hemalatha, M.; Thomas, R. G.; Mahata, K.; Kailas, S.; Gupta, A. K.

    2016-01-01

    Recently, we have performed systematic Secondary Ion Mass Spectrometry (SIMS) measurements at our ion source test set up and have demonstrated that gas phase 32SFn- (n = 1-6) anions for all size 'n' can be readily generated from a variety of surfaces undergoing Cs+ ion sputtering in the presence of high purity SF6 gas by employing the gas spray-cesium sputter technique. In our SIMS measurements, the isotopic yield ratio 34SFn-/32SFn- (n = 1-6) was found to be close to its natural abundance but not for all size 'n'. In order to gain further insight into the constituents of these molecular anions, ultra sensitive Accelerator Mass Spectrometry (AMS) measurements were conducted with the most abundant 32SFn- (n = 1-6) anions, at BARC-TIFR 14 UD Pelletron accelerator. The results from these measurements are discussed in this paper.

  16. 1-Methoxypyrene and 1,6-dimethoxypyrene: two novel metabolites in fungal metabolism of polycyclic aromatic hydrocarbons.

    Science.gov (United States)

    Wunder, T; Marr, J; Kremer, S; Sterner, O; Anke, H

    1997-05-01

    The metabolism of pyrene by Penicillium glabrum strain TW 9424, a strain isolated from a site contaminated with polycyclic aromatic hydrocarbons (PAHs) was investigated in submerged cultures. The metabolites formed were identified as 1-hydroxypyrene, 1,6- and 1, 8-dihydroxypyrene, 1,6- and 1,8-pyrenequinone, and 1-pyrenyl sulfate. In addition, two new metabolites were isolated and identified by UV, 1H nuclear magnetic resonance, and mass spectroscopy as 1-methoxypyrene and 1,6-dimethoxypyrene. Experiments with [methyl-3H]S-adenosyl-l-methionine (SAM) revealed that SAM is the coenzyme that provides the methyl group for the methyltransferase involved. To our knowledge, this is the first time that methoxylated metabolites of PAHs have been isolated from fungal cultures.

  17. Sialylated β1, 6 branched N-glycans modulate the adhesion, invasion and metastasis of hepatocarcinoma cells.

    Science.gov (United States)

    Yu, Xiao; Zhao, Yujie; Wang, Liping; Chen, Xixi; Su, Zhen; Zhang, Han; Yuan, Qingmin; Wang, Shujing

    2016-12-01

    The mouse hepatocarcinoma cell lines Hca-F and Hca-P have been derived from hepatocarcinoma in mice and metastasize only to the lymph node. Hca-F cells displayed greater lymphatic metastasis ability than Hca-P cells. When the two cell lines were compared for cell surface sialylated β1,6 branched N-glycans by flow cytometry using L-PHA and SNA, Hca-F cells were found to express significantly higher levels. To explore the effect of increased sialylated β1,6 branched N-glycans on hepatocarcinoma progression, we inhibit their expression in Hca-F cells by using swainsonine treatment and RNA interference. We found that swainsonine treatment or GnT-V-shRNA transfection significantly inhibited the formation of β1,6 branched N-glycans, and partially inhibited the expression of α2,6 sialic acids. Knockdown of sialylated β1,6 branched N-glycans significantly attenuated the invasive and metastatic capability both in vitro and in vivo. Blockade of α2,6 sialic acid expression on Hca-F cell surface by the treatment with neuraminidase caused reduction in cellular adherence to lymph node. In addition, knockdown of sialylated β1,6 branched N-glycans could decrease the expression of Notch1, NICD1, NICD2 and HES1 in Hca-F cells. Collectively, these findings suggest that increased sialylated β1,6 branched N-glycans may contribute to hepatocarcinoma progression by altering the adhesive, invasive and metastatic ability to lymph node via Notch signaling pathway.

  18. Sodium channel SCN8A (Nav1.6: properties and de novo mutations in epileptic encephalopathy and intellectual disability

    Directory of Open Access Journals (Sweden)

    Janelle Elizabeth O'Brien

    2013-10-01

    Full Text Available The sodium channel Nav1.6, encoded by the gene SCN8A, is one of the major voltage-gated channels in human brain. The sequences of sodium channels have been highly conserved during evolution, and minor changes in biophysical properties can have a major impact in vivo. Insight into the role of Nav1.6 has come from analysis of spontaneous and induced mutations of mouse Scn8a during the past 18 years. Only within the past year has the role of SCN8A in human disease become apparent from whole exome and genome sequences of patients with sporadic disease. Unique features of Nav1.6 include its contribution to persistent current, resurgent current, repetitive neuronal firing, and subcellular localization at the axon initial segment and nodes of Ranvier. Loss of Nav1.6 activity results in reduced neuronal excitability, while gain-of-function mutations can increase neuronal excitability. Mouse Scn8a (med mutants exhibit movement disorders including ataxia, tremor and dystonia. Thus far, more than ten human de novo mutations have been identified in patients with two types of disorders, epileptic encephalopathy and intellectual disability. We review these human mutations as well as the unique features of Nav1.6 that contribute to its role in determining neuronal excitability in vivo. A supplemental figure illustrating the positions of amino acid residues within the 4 domains and 24 transmembrane segments of Nav1.6 is provided to facilitate the location of novel mutations within the channel protein.

  19. Structure of a Class I Tagatose-1,6-bisphosphate Aldolase - Investigation into an Apparent Loss of Stereospecificity

    Energy Technology Data Exchange (ETDEWEB)

    LowKam, C.; Liotard, B; Sygusch, J

    2010-01-01

    Tagatose-1,6-bisphosphate aldolase from Streptococcus pyogenes is a class I aldolase that exhibits a remarkable lack of chiral discrimination with respect to the configuration of hydroxyl groups at both C3 and C4 positions. The enzyme catalyzes the reversible cleavage of four diastereoisomers (fructose 1,6-bisphosphate (FBP), psicose 1,6-bisphosphate, sorbose 1,6-bisphosphate, and tagatose 1,6-bisphosphate) to dihydroxyacetone phosphate (DHAP) and d-glyceraldehyde 3-phosphate with high catalytic efficiency. To investigate its enzymatic mechanism, high resolution crystal structures were determined of both native enzyme and native enzyme in complex with dihydroxyacetone-P. The electron density map revealed a ({alpha}/{beta}){sub 8} fold in each dimeric subunit. Flash-cooled crystals of native enzyme soaked with dihydroxyacetone phosphate trapped a covalent intermediate with carbanionic character at Lys{sup 205}, different from the enamine mesomer bound in stereospecific class I FBP aldolase. Structural analysis indicates extensive active site conservation with respect to class I FBP aldolases, including conserved conformational responses to DHAP binding and conserved stereospecific proton transfer at the DHAP C3 carbon mediated by a proximal water molecule. Exchange reactions with tritiated water and tritium-labeled DHAP at C3 hydrogen were carried out in both solution and crystalline state to assess stereochemical control at C3. The kinetic studies show labeling at both pro-R and pro-S C3 positions of DHAP yet detritiation only at the C3 pro-S-labeled position. Detritiation of the C3 pro-R label was not detected and is consistent with preferential cis-trans isomerism about the C2-C3 bond in the carbanion as the mechanism responsible for C3 epimerization in tagatose-1,6-bisphosphate aldolase.

  20. 3,4-Dihydroxy-1,6-bis(4-methoxyphenyl)hexa-2,4-diene-1,6-dione, its 4-methylphenyl analogue, and a potassium salt of 2-hydroxy-4-(4-methoxyphenyl)-4-oxobut-2-enoic acid.

    Science.gov (United States)

    Nye, Luke; Turnbull, Mark M; Wikaira, Jan L

    2013-11-01

    Reaction of 4-methoxyacetophenone with diethyl oxalate under basic conditions produced 3,4-dihydroxy-1,6-bis(4-methoxyphenyl)hexa-2,4-diene-1,6-dione, C20H18O6, (1). The molecules lie across a crystallographic inversion centre and intramolecular hydrogen bonding, similar to acetylacetone, is observed, confirming that the molecule is in the di-enol-dione tautomeric form. Additional O-H...O hydrogen bonds link the molecules into chains parallel to the b axis. The structure is compared with that of redetermined 4-methylphenyl compound 3,4-dihydroxy-1,6-bis(4-methylphenyl)hexa-2,4-diene-1,6-dione, C20H18O4, (2), which crystallizes in a similar fashion. The salt, catena-poly[[μ2-2-hydroxy-4-(4-methoxyphenyl)-4-oxobut-2-enoato-κ(3)O(1),O(2):O(4)][μ2-2-hydroxy-4-(4-methoxyphenyl)-4-oxobut-2-enoic acid-κ(2)O(1):O(4)]potassium], [K(C11H9O5)(C11H10O5)]n, (3), was isolated as a by-product of the synthesis of (1). The two organic species are linked by a strong hydrogen bond between the carboxylic acid and carboxylate groups. They are further stabilized and linked into a double-chain structure via the seven-coordinate potassium ion.

  1. Synthesis with Nitriles: Synthesis of Some New Mercaptopyridazine, Mercaptopyridazino[1,6-a]quinazoline and Thiophene Derivatives

    Directory of Open Access Journals (Sweden)

    Mariam A. Al-Sheikh

    2008-11-01

    Full Text Available 2-(1-(4-Bromophenyl-2-thiocyanatoethylidenemalononitrile (3 undergoes azo coupling with diazotized aromatic amines to afford arylhydrazone derivatives, which are readily cyclized to afford the corresponding 3(2H-pyridazinimine derivatives upon reflux in aqueous NaOH. Under similar condition an o-cyanoarylhydrazone derivative was cyclized into 6H-pyridazino[1,6-a]quinazolin-6-imine, which in turn was easily transformed into 6H-pyridazino[1,6-a]quinazolin-6-one on reflux in ethanolic/HCl. Compound 3 afforded substituted 5-acetylthiophene derivatives upon reflux in AcOH/HCl mixtures.

  2. Three-transition cascade erbium laser at 1.7, 2.7, and 1.6 µm

    NARCIS (Netherlands)

    Pollnau, Markus; Ghisler, C.; Lüthy, W.; Weber, H.P.; Schneider, J.; Unrau, U.B.

    1997-01-01

    We report on an upconversion cascade laser in an erbium-doped ZBLAN fiber emitting simultaneously on the three transitions 4S3/2 -> 4I9/2 at 1.7 um, 4I11/2 -> 4I13/2 at 2.7 um, and 4I13/2 -> 4I15/2 at 1.6 um. At moderate pump powers, the laser transition at 1.6 um supports 2.7-um lasing and permits

  3. Hydrogen bonds and van der Waals forces as tools for the construction of a herringbone pattern in the crystal structure of hexane-1,6-diaminium hexane-1,6-diyl bis­(hydrogen phospho­nate)

    Science.gov (United States)

    Reiss, Guido J.; van Megen, Martin; Frank, Walter

    2017-01-01

    The asymmetric unit of the title salt, [H3N(CH2)6NH3][(HO)O2P(CH2)6PO2(OH)], consists of one half of a hexane-1,6-diaminium dication and one half of a hexane-1,6-diyl bis­(hydrogen phospho­nate) dianion. Both are located around different centres of inversion (Wyckoff sites: 2a and 2d) of the space group P21/c. The shape of the hexane-1,6-diaminium cation is best described as a double hook. Both aminium groups as well as the two attached CH2 groups are turned out from the plane of the central four C atoms. In contrast, all six C atoms of the dianion are almost in a plane. The hydrogen phospho­nate (–PO3H) groups of the anions and the aminium groups of the cations form two-dimensional O—H⋯ and O—H⋯N hydrogen-bonded networks parallel to the ac plane, built up from ten-membered and twelve-membered ring motifs with graph-set descriptors R 3 3(10) and R 5 4(12), respectively. These networks are linked by the alkyl­ene chains of the anions and cations. The resulting three-dimensional network shows a herringbone pattern, which resembles the parent structures 1,6-di­amino­hexane and hexane-1,6-di­phospho­nic acid. PMID:28083141

  4. Complete genome sequence of Bacillus amyloliquefaciens strain Co1-6, a plant growth-promoting rhizobacterium of Calendula officinalis

    Energy Technology Data Exchange (ETDEWEB)

    Koeberl, Martina; White, Richard A.; Erschen, Sabine; Spanberger, Nora; El-Arabi, Tarek F.; Jansson, Janet K.; Berg, Gabriele

    2015-08-13

    The genome sequence of Bacillus amyloliquefaciens strain Co1-6, a plant growth-promoting rhizobacterium (PGPR) with broad-spectrum antagonistic activities against plant pathogenic fungi, bacteria and nematodes, consists of a single 3.9 Mb circular chromosome. The genome reveals genes putatively responsible for its promising biocontrol and PGP properties.

  5. Intermolecular gold-catalyzed diastereo- and enantioselective [2+2+3] cycloadditions of 1,6-enynes with nitrones.

    Science.gov (United States)

    Gawade, Sagar Ashok; Bhunia, Sabyasachi; Liu, Rai-Shung

    2012-07-27

    Going for gold: The title reaction has been developed and demonstrates a wide substrate scope with respect to the 1,6-enynes and nitrones (see scheme; DCE = 1,2-dichloroethane, Tf = trifluoromethanesulfonyl). The results for the enantioselective versions are also presented.

  6. Aberrant epilepsy-associated mutant Nav1.6 sodium channel activity can be targeted with cannabidiol

    OpenAIRE

    Patel, Reesha R.; Barbosa, Cindy; Brustovetsky, Tatiana; Brustovetsky, Nickolay; Cummins, Theodore R.

    2016-01-01

    Resurgent sodium currents arise from channel reopening during repolarisation, and are predicted to increase neuronal excitability. Patel et al. show that epilepsy-associated mutations in the voltage-gated sodium channel Nav1.6, but not Nav1.1, upregulate resurgent currents. Cannabidiol preferentially targets these currents, suggesting a strategy for reducing neuronal hyperexcitability associated with epilepsy.

  7. Preferential targeting of Nav1.6 voltage-gated Na+ Channels to the axon initial segment during development.

    Directory of Open Access Journals (Sweden)

    Elizabeth J Akin

    Full Text Available During axonal maturation, voltage-gated sodium (Nav channels accumulate at the axon initial segment (AIS at high concentrations. This localization is necessary for the efficient initiation of action potentials. The mechanisms underlying channel trafficking to the AIS during axonal development have remained elusive due to a lack of Nav reagents suitable for high resolution imaging of channels located specifically on the cell surface. Using an optical pulse-chase approach in combination with a novel Nav1.6 construct containing an extracellular biotinylation domain we demonstrate that Nav1.6 channels are preferentially inserted into the AIS membrane during neuronal development via direct vesicular trafficking. Single-molecule tracking illustrates that axonal channels are immediately immobilized following delivery, while channels delivered to the soma are often mobile. Neither a Nav1.6 channel lacking the ankyrin-binding motif nor a chimeric Kv2.1 channel containing the Nav ankyrinG-binding domain show preferential AIS insertion. Together these data support a model where ankyrinG-binding is required for preferential Nav1.6 insertion into the AIS plasma membrane. In contrast, ankyrinG-binding alone does not confer the preferential delivery of proteins to the AIS.

  8. The pion-kaon scattering amplitude constrained with forward dispersion relations up to 1.6 GeV

    CERN Document Server

    Pelaez, J R

    2016-01-01

    In this work we provide simple and precise parameterizations of the existing $\\pi K$ scattering data from threshold up to 1.6 GeV, which are constrained to satisfy forward dispersion relations as well as three additional threshold sum rules. We also provide phenomenological values of the threshold parameters and of the resonance poles that appear in elastic scattering.

  9. A Gain-of-Function Mutation in Nav1.6 in a Case of Trigeminal Neuralgia

    Science.gov (United States)

    Tanaka, Brian S; Zhao, Peng; Dib-Hajj, Fadia B; Morisset, Valerie; Tate, Simon; Waxman, Stephen G; Dib-Hajj, Sulayman D

    2016-01-01

    Idiopathic trigeminal neuralgia (TN) is a debilitating pain disorder characterized by episodic unilateral facial pain along the territory of branches of the trigeminal nerve. Human pain disorders, but not TN, have been linked to gain-of-function mutations in peripheral voltage-gated sodium channels (NaV1.7, NaV1.8 and NaV1.9). Gain-of-function mutations in NaV1.6, which is expressed in myelinated and unmyelinated central nervous system (CNS) and peripheral nervous system neurons and supports neuronal high-frequency firing, have been linked to epilepsy but not to pain. Here, we describe an individual who presented with evoked and spontaneous paroxysmal unilateral facial pain and carried a diagnosis of TN. Magnetic resonance imaging showed unilateral neurovascular compression, consistent with pain in areas innervated by the second branch of the trigeminal nerve. Genetic analysis as part of a phase 2 clinical study in patients with TN conducted by Convergence Pharmaceuticals Ltd revealed a previously undescribed de novo missense mutation in NaV1.6 (c.A406G; p.Met136Val). Whole-cell voltage-clamp recordings show that the Met136Val mutation significantly increases peak current density (1.5-fold) and resurgent current (1.6-fold) without altering gating properties. Current-clamp studies in trigeminal ganglia (TRG) neurons showed that Met136Val increased the fraction of high-firing neurons, lowered the current threshold and increased the frequency of evoked action potentials in response to graded stimuli. Our results demonstrate a novel NaV1.6 mutation in TN, and show that this mutation potentiates transient and resurgent sodium currents and leads to increased excitability in TRG neurons. We suggest that this gain-of-function NaV1.6 mutation may exacerbate the pathophysiology of vascular compression and contribute to TN. PMID:27496104

  10. 氨基甲酸酯裂解制HDI热力学分析%Thermodynamic analysis of catalytic decomposition of dimethyl-hexane-1,6-dicarbamate to hexamethylene-1,6-diisocyanate

    Institute of Scientific and Technical Information of China (English)

    孙大雷; 黄振荣; 黄宇嘉; 邓剑如; 晁自胜

    2013-01-01

    The catalytic decomposition of dimethyl-hexane-1,6-dicarbamate (HDU) to hexamethylene-1,6-diisocyanate (HDI) was a complex reaction system.The reaction heat,Gibbs free energy change and equilibrium constant of the reactions were calculated by methods of group contribution.The estimated data were compared with the literature and experimental data,and the results showed that they are reliable,which could be used to guide the laboratory research and industrial production.%由六亚甲基二氨基二甲酸甲酯(HDU)裂解合成六亚甲基-1,6-二异氰酸酯(HDI)为一复合反应体系.用基团贡献法计算了该反应体系的反应热、吉布斯自由能变化、化学反应平衡常数.计算数据与文献值及实验结果比较,表明计算结果可靠,对实验室研究及工业化生产都有重要的指导意义.

  11. One-Pot Synthesis of Dialkyl Hexane-1,6-Dicarbamate from 1,6-Hexanediamine, Urea, and Alcohol over Zinc-Incorporated Berlinite (ZnAlPO4 Catalyst

    Directory of Open Access Journals (Sweden)

    Da-Lei Sun

    2016-02-01

    Full Text Available Dialkyl hexane-1,6-dicarbamate was synthesized, for the first time, by a one-pot reaction of 1,6-hexanediamine (HDA, urea, and alcohols, including methanol, ethanol, propanol, and butanol, in a self-designed batch reactor, using zinc-incorporated berlinite (ZnAlPO4 as a catalyst. The yield of dibutyl hexane-1,6-dicarbamate (2 was systematically investigated as a function of Zn/Al molar ratio, reaction temperature, reaction time, catalyst usage and urea/HDA/butanol molar ratio. Based on these studies, the optimized reaction conditions were as follows: molar ratio urea/HDA/butanol = 2.6:1:8.6, catalyst usage = 3.0 g, reaction temperature = 493 K, reaction time = 6 h and reaction pressure = 1.2 MPa; a yield of 2 of 89.7% was achieved over the ZnAlPO4 (molar ratio Zn/Al = 0.04 catalyst. The catalysts were characterized by X-ray photoelectric spectroscopy (XPS and scanning electron microscope (SEM. Additionally, based on these experimental results, it was also proposed that the catalysis recycle of the one-pot synthesis of 2 from urea, HDA, and butanol over the ZnAlPO4 catalyst.

  12. Structures of NodZ α1,6-fucosyltransferase in complex with GDP and GDP-fucose

    Energy Technology Data Exchange (ETDEWEB)

    Brzezinski, Krzysztof [Argonne National Laboratory, Argonne, IL 60439 (United States); Polish Academy of Sciences, 61-704 Poznan (Poland); Dauter, Zbigniew [Argonne National Laboratory, Argonne, IL 60439 (United States); Jaskolski, Mariusz, E-mail: mariuszj@amu.edu.pl [Polish Academy of Sciences, 61-704 Poznan (Poland); A. Mickiewicz University, 60-780 Poznan (Poland); Argonne National Laboratory, Argonne, IL 60439 (United States)

    2012-02-01

    Crystal structures of the bacterial α1,6-fucosyltransferase NodZ in complex with GDP and GDP-fucose are presented. Rhizobial NodZ α1,6-fucosyltransferase (α1,6-FucT) catalyzes the transfer of the fucose (Fuc) moiety from guanosine 5′-diphosphate-β-l-fucose to the reducing end of the chitin oligosaccharide core during Nod-factor (NF) biosynthesis. NF is a key signalling molecule required for successful symbiosis with a legume host for atmospheric nitrogen fixation. To date, only two α1,6-FucT structures have been determined, both without any donor or acceptor molecule that could highlight the structural background of the catalytic mechanism. Here, the first crystal structures of α1,6-FucT in complex with its substrate GDP-Fuc and with GDP, which is a byproduct of the enzymatic reaction, are presented. The crystal of the complex with GDP-Fuc was obtained through soaking of native NodZ crystals with the ligand and its structure has been determined at 2.35 Å resolution. The fucose residue is exposed to solvent and is disordered. The enzyme–product complex crystal was obtained by cocrystallization with GDP and an acceptor molecule, penta-N-acetyl-l-glucosamine (penta-NAG). The structure has been determined at 1.98 Å resolution, showing that only the GDP molecule is present in the complex. In both structures the ligands are located in a cleft formed between the two domains of NodZ and extend towards the C-terminal domain, but their conformations differ significantly. The structures revealed that residues in three regions of the C-terminal domain, which are conserved among α1,2-, α1,6- and protein O-fucosyltransferases, are involved in interactions with the sugar-donor molecule. There is also an interaction with the side chain of Tyr45 in the N-terminal domain, which is very unusual for a GT-B-type glycosyltransferase. Only minor conformational changes of the protein backbone are observed upon ligand binding. The only exception is a movement of the loop

  13. Catalysis over zinc-incorporated berlinite (ZnAlPO4 of the methoxycarbonylation of 1,6-hexanediamine with dimethyl carbonate to form dimethylhexane-1,6-dicarbamate

    Directory of Open Access Journals (Sweden)

    Sun Da-Lei

    2007-11-01

    Full Text Available Abstract Background The alkoxycarbonylation of diamines with dialkyl carbonates presents promising route for the synthesis of dicarbamates, one that is potentially 'greener' owing to the lack of a reliance on phosgene. While a few homogeneous catalysts have been reported, no heterogeneous catalyst could be found in the literature for use in the synthesis of dicarbamates from diamines and dialkyl carbonates. Because heterogeneous catalysts are more manageable than homogeneous catalysts as regards separation and recycling, in our study, we hydrothermally synthesized and used pure berlinite (AlPO4 and zinc-incorporated berlinite (ZnAlPO4 as heterogeneous catalysts in the production of dimethylhexane-1,6-dicarbamate from 1,6-hexanediamine (HDA and dimethyl carbonate (DMC. The catalysts were characterized by means of XRD, FT-IR and XPS. Various influencing factors, such as the HDA/DMC molar ratio, reaction temperature, reaction time, and ZnAlPO4/HDA ratio, were investigated systematically. Results The XRD characterization identified a berlinite structure associated with both the AlPO4 and ZnAlPO4 catalysts. The FT-IR result confirmed the incorporation of zinc into the berlinite framework for ZnAlPO4. The XPS measurement revealed that the zinc ions in the ZnAlPO4 structure possessed a higher binding energy than those in ZnO, and as a result, a greater electron-attracting ability. It was found that ZnAlPO4 catalyzed the formation of dimethylhexane-1,6-dicarbamate from the methoxycarbonylation of HDA with DMC, while no activity was detected on using AlPO4. Under optimum reaction conditions (i.e. a DMC/HDA molar ratio of 8:1, reaction temperature of 349 K, reaction time of 8 h, and ZnAlPO4/HDA ratio of 5 (mg/mmol, a yield of up to 92.5% of dimethylhexane-1,6-dicarbamate (with almost 100% conversion of HDA was obtained. Based on these results, a possible mechanism for the methoxycarbonylation over ZnAlPO4 was also proposed. Conclusion As a heterogeneous

  14. 1 - 6_Dabo

    African Journals Online (AJOL)

    DR. AMINU

    screened for their in vitro anti-plasmodial activities against laboratory adapted chloroquine susceptible ... the leaves of C. aurantifolia, Carica papaya, Mangifera indica and Psidium guajava) were further screened ... Citrus aurantifolia. 20/07.

  15. Potentiation of histamine release by Microfungal (1-->3)- and (1-->6)-beta-D-glucans

    DEFF Research Database (Denmark)

    Holck, Peter; Sletmoen, Marit; Stokke, Bjørn Torger

    2007-01-01

    : curdlan [a linear (1-->3)-beta-D-glucan], laminarin and scleroglucan, and furthermore with pustulan, a linear (1-->6)-beta-D-glucan. Histamine release was not observed on exposure to the glucans only, but in the presence of anti-immunoglobulin E (IgE) antibody or specific antigens, all the glucans...... investigated led to an enhancement of the IgE-mediated histamine release. The glucans induced a significant potentiation of the mediator release when present at concentrations in the range of 2-5 x 10(-5) M. These results suggest that (1-->3)-beta-D-glucan as well as (1-->6)-beta-D-glucan aggravates Ig......E-mediated histamine release. Knowledge concerning the effects of glucans on immune responses may be of importance for understanding and treating inflammatory and allergic diseases....

  16. Demonstration of fiber pulsed light source at 1.6 μm with adjustable pulse duration

    Institute of Scientific and Technical Information of China (English)

    Xue Feng; Lei Zhang; Xiaoming Liu

    2007-01-01

    A novel practical 1.66-μm pulse light source with adjustable pulse duration is proposed. A 2.5-km Raman fiber is placed into a ring type Q-switched erbium-doped fiber laser (Q-EDFL), serving as both delay line fiber and Raman gain medium so that in addition to the wavelength shifted to 1.6μm, the pulse duration and the buildup time can be relatively extended. By properly controlling the fall edge of the acousto-optic switch (AOS), the pulse duration of 30-345 ns for ~ 770-Hz repetition frequency with power of 1-1.6 W is achieved.

  17. 2,6 Sialylation associated with increased 1,6-branched -oligosaccharides influences cellular adhesion and invasion

    Indian Academy of Sciences (India)

    Amit Ranjan; Rajiv D Kalraiya

    2013-12-01

    Expression of 1,6-branched N-linked oligosaccharides have a definite association with invasion and metastasis of cancer cells. However, the mechanism by which these oligosaccharides regulate these processes is not well understood. Invasive variants of B16 murine melanoma, B16F10 (parent) and B16BL6 (highly invasive variant) cell lines have been used for these studies. We demonstrate that substitution of 2,6-linked sialic acids on multiantennary structures formed as a result of 1,6-branching modulate cellular adhesion on both extracellular matrix (ECM) and basement membrane (BM) components. Removal of 2,6 sialic acids either by enzymatic desialylation or by stably down-regulating the ST6Gal-I (enzyme that catalyses the addition of 2,6-linked sialic acids on N-linked oligosaccharides) by lentiviral driven shRNA decreased the adhesion on both ECM and BM components and invasion through reconstituted BM matrigel.

  18. Role of histamine H3 receptor in glucagon-secreting αTC1.6 cells.

    Science.gov (United States)

    Nakamura, Tadaho; Yoshikawa, Takeo; Naganuma, Fumito; Mohsen, Attayeb; Iida, Tomomitsu; Miura, Yamato; Sugawara, Akira; Yanai, Kazuhiko

    2015-01-01

    Pancreatic α-cells secrete glucagon to maintain energy homeostasis. Although histamine has an important role in energy homeostasis, the expression and function of histamine receptors in pancreatic α-cells remains unknown. We found that the histamine H3 receptor (H3R) was expressed in mouse pancreatic α-cells and αTC1.6 cells, a mouse pancreatic α-cell line. H3R inhibited glucagon secretion from αTC1.6 cells by inhibiting an increase in intracellular Ca(2+) concentration. We also found that immepip, a selective H3R agonist, decreased serum glucagon concentration in rats. These results suggest that H3R modulates glucagon secretion from pancreatic α-cells.

  19. Measurement of Lower-Atmospheric CO2 Concentration Distribution Using a Compact 1.6 μm DIAL

    Science.gov (United States)

    Shibata, Yasukuni; Nagasawa, Chikao; Abo, Makoto

    2016-06-01

    Knowledge of present carbon sources and sinks including their spatial distribution and their variation in time is one of the essential information for predicting future CO2 atmospheric concentration levels. The differential absorption lidar (DIAL) is expected to measure atmospheric CO2 profiles in the atmospheric boundary layer and lower troposphere from a ground platform. We have succeeded to develop a compact 1.6 μm DIAL system for measuring CO2 concentration profiles in the lower atmosphere. This 1.6 μm DIAL system consists of the optical parametric generator (OPG) transmitter that excited by the LD pumped Nd:YAG laser with high repetition rate and the receiving optics that included the near-infrared photomultiplier tube operating at the analog mode and a 25 cm telescope. CO2 concentration profiles were obtained up to 2.5 km altitude.

  20. Synthesis and antifungal activity of 7-methyl-7-hydroxy-2,3-benzo[c]octa-1,6-olide.

    Science.gov (United States)

    Zhao, Jin; Dong, Hong-Bo; Yang, Ming-Yan; Du, Juan; Jiang, Jia-Zheng; Wang, Ming-An

    2014-01-01

    The racemic 7-methyl-7-hydroxy-2,3-benzo[c]octa-1,6-olide, the analog of natural product (6R)-3,7-dimethyl-7-hydroxy-2-octen-1,6-olide, was totally synthesized using easily available (E)-2-(2-carboxyvinyl)benzoic acid as a raw material in nine-step reactions including three key steps of Wittig reaction, epoxidation, and cyclization, with an overall yield of 10.3%. The bioassay results showed that ( ± )-2 exhibited stronger antifungal activity than the natural product ( ± )-1 and (R)-1 against Alternaria solani with an EC₅₀ value of 27.36 μg/ml.

  1. High-power pulsed laser diodes emitting in the range 1.5 – 1.6 μm

    Energy Technology Data Exchange (ETDEWEB)

    Gorlachuk, P V; Ryaboshtan, Yu L; Ladugin, M A; Padalitsa, A A; Marmalyuk, A A; Kurnosov, V D; Kurnosov, K V; Zhuravleva, O V; Romantsevich, V I; Chernov, R V; Ivanov, A V; Simakov, V A [Open Joint-Stock Company M.F. Stel' makh Polyus Research Institute, Moscow (Russian Federation)

    2013-09-30

    This paper examines approaches for increasing the output pulse power of laser diodes based on MOVPE InGaAs/AlGaInAs/InP heterostructures and emitting in the range 1.5 – 1.6 μm. We demonstrate that optimising waveguide layer parameters may ensure an increase in the quantum efficiency of the laser diodes and a reduction in their internal optical loss. Characterisation results are presented for laser diodes based on the proposed heterostructures. (lasers)

  2. Coatings prepared from waterborne polyurethane dispersions obtained with polycarbonates of 1,6-hexanediol of different molecular weights

    OpenAIRE

    García Pacios, Vanesa; Jofre-Reche, José Antonio; Costa, Víctor; Colera, Manuel; Martín-Martínez, José Miguel

    2013-01-01

    Waterborne polyurethane dispersions (PUDs) were synthesized with polycarbonates of 1,6-hexanediol of different molecular weight (500–3000 Da) and their properties, adhesion (Hatch adhesion) and coatings on stainless steel properties (Pencil hardness, Persoz hardness, gloss at 60°, chemical resistance, yellowness index) were characterized. The hatch adhesion of the polyurethane coatings to stainless steel was very good and decreased slightly by increasing the molecular weight of the polycarbon...

  3. Energy densities of Alfven waves between 0.7 and 1.6 AU. [in interplanetary medium

    Science.gov (United States)

    Belcher, J. W.; Burchsted, R.

    1974-01-01

    Plasma and field data from Mariner 4 and 5 between 0.7 and 1.6 AU are used to study the radial dependence of the levels of microscale fluctuation associated with interplanetary Alfven waves. The observed decrease of these levels with increasing distance from the sun is consistent with little or no local generation or damping of the ambient Alfven waves over this range of radial distance.

  4. Coatings prepared from waterborne polyurethane dispersions obtained with polycarbonates of 1,6-hexanediol of different molecular weights

    OpenAIRE

    García Pacios, Vanesa; Jofre-Reche, José Antonio; Costa, Víctor; Colera, Manuel; Martín-Martínez, José Miguel

    2013-01-01

    Waterborne polyurethane dispersions (PUDs) were synthesized with polycarbonates of 1,6-hexanediol of different molecular weight (500–3000 Da) and their properties, adhesion (Hatch adhesion) and coatings on stainless steel properties (Pencil hardness, Persoz hardness, gloss at 60°, chemical resistance, yellowness index) were characterized. The hatch adhesion of the polyurethane coatings to stainless steel was very good and decreased slightly by increasing the molecular weight of the polycarbon...

  5. 1,6-asymmetric induction in boron-mediated aldol reactions: application to a practical total synthesis of (+)-discodermolide.

    Science.gov (United States)

    Paterson, Ian; Delgado, Oscar; Florence, Gordon J; Lyothier, Isabelle; Scott, Jeremy P; Sereinig, Natascha

    2003-01-01

    By relying solely on substrate-based stereocontrol, a practical total synthesis of the microtubule-stabilizing anticancer agent (+)-discodermolide has been realized. This exploits a novel aldol bond construction with 1,6-stereoinduction from the boron enolate of (Z)-enone 3 in addition to aldehyde 2. The 1,3-diol 7 is employed as a common building block for the C(1)-C(5), C(9)-C(16), and C(17)-C(24) subunits. [reaction--see text

  6. Saccharomyces pastorianus as cell factory to improve production of fructose 1,6-diphosphate using novel fermentation strategies

    Directory of Open Access Journals (Sweden)

    Chiara Schiraldi

    2015-08-01

    Full Text Available Enzymatic phosphorylation of glucose with inorganic phosphate, mediated by permeabilized yeast cells, is one of the methods commonly used to manufacture fructose 1,6-diphosphate, a compound of pharmaceutical interest. This process requires high concentrations of yeast active biomass, that is the catalyst of bioconversion of glucose and inorganic phosphate into fructose 1,6-diphosphate. In this study we firstly describe the high cell density production of a brewer's Saccharomyces strain (Saccharomyces pastorianus DSM 6581, focusing on the optimization of medium composition and exploiting fed-batch strategies and novel technologies based on membrane bioreactors. In fed-batch fermentation an appropriate exponential feed profile was set up to maintain the glucose concentration in the bioreactor below 0.9 g·L-1, thus yielding reproducibly 58 g dry weight biomass per liter in 80 h fermentation, improving eight-fold batch processes output. In addition a higher final biomass density was reached when implementing a microfiltration strategy (70 g dry weight biomass, that led to a productivity of 2.1 gcdw·L-1·h-1, 2.4-fold the fed-batch one. Successively, this biomass was opportunely permeabilized and proved capable of catalyzing the bioconversion of glucose into fructose 1,6-diphosphate. Acting on critical parameters of the bioconversion (substrates molar ratio, catalyst concentration and permeabilization agent, fructose 1,6-diphosphate was produced, after 3 h of process, at 56.3 ± 1 g·L-1 with a yield of 80% of the theoretical value.

  7. Metamorphic InGaAs quantum wells for light emission at 1.3-1.6 {mu}m

    Energy Technology Data Exchange (ETDEWEB)

    Wang, S.M. [Department of Microtechnology and Nanoscience, Chalmers University of Technology, 41296 Goeteborg (Sweden)]. E-mail: shumin.wang@mc2.chalmers.se; Tangring, I. [Department of Microtechnology and Nanoscience, Chalmers University of Technology, 41296 Goeteborg (Sweden); Gu, Q.F. [Department of Microtechnology and Nanoscience, Chalmers University of Technology, 41296 Goeteborg (Sweden); Sadeghi, M. [Department of Microtechnology and Nanoscience, Chalmers University of Technology, 41296 Goeteborg (Sweden); Larsson, A. [Department of Microtechnology and Nanoscience, Chalmers University of Technology, 41296 Goeteborg (Sweden); Wang, X.D. [Engineering Research Center for Semiconductor Integrated Technology, Institute of Semiconductors, Chinese Academy of Sciences, P. O. Box 912, 100083 Beijing (China); Ma, C.H. [Engineering Research Center for Semiconductor Integrated Technology, Institute of Semiconductors, Chinese Academy of Sciences, P. O. Box 912, 100083 Beijing (China); Buyanova, I.A. [Department of Physics and Measurement Technology, Linkoeping University, 58183 Linkoeping (Sweden); Chen, W.M. [Department of Physics and Measurement Technology, Linkoeping University, 58183 Linkoeping (Sweden)

    2007-03-26

    Metamorphic InGaAs quantum well structures grown on GaAs reveal strong light emission at 1.3-1.6 {mu}m, smooth surface with an average roughness below 2 nm and good rectifying I-V characteristics. Dark line defects are found in the QW. Post growth thermal annealing further improves the luminescence efficiency but does not remove those dark line defects. Some challenges of epitaxial growth using this method for laser applications are discussed.

  8. A 1.6-kW, 110-kHz dc-dc converter optimized for IGBT's

    Science.gov (United States)

    Chen, Keming; Stuart, Thomas A.

    1993-01-01

    A full bridge dc-dc converter using a zero-current and zero-voltage switching technique is described. This circuit utilizes the characteristics of the IGBT to achieve power and frequency combinations that are much higher than previously reported for this device. Experimental results are included for a 1.6-kW, 110-kHz converter with 95 percent efficiency.

  9. Thermal Analysis of Post-eruption Loops from 80,000 to 1.6 million K

    Science.gov (United States)

    Kucera, T.; Landi, E.

    2006-01-01

    We analyze the thermal properties of a set of post eruptive loops which appeared after a prominence eruption on April 30, 2004. The event was observed by TRACE and SOHO/SUMER. The SUMER data was taken from a single slit location with a 90 second cadence and included a number of lines spanning the temperature range 80,000 to 1.6 million K. We perform a differential emission measure analysis of the loops in order to study their thermal evolution.

  10. Double cascade erbium fiber laser at 1.7 µm, 2.7 µm, and 1.6 µm

    NARCIS (Netherlands)

    Schneider, J.; Frerichs, Ch.; Carbonnier, C.; Unrau, U.B.; Pollnau, Markus; Lüthy, W.; Weber, H.P.

    The output power of the erbium laser at 2.7 um (4I11/2 -> 4I13/2) is enhanced due to simultaneous laser action at 1.7 um (4S3/2 -> 4I9/2) and 1.6 um (4I13/2 -> 4I15/2) in an Er3+-doped fluorozirconate fiber. The laser cascade overwhelms the saturation effect for the transition at 2.7 um by

  11. Microstructural Evolution of 2.25Cr-1.6W-V-Nb Heat Resistant Steel during Creep

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    2.25Cr-1.6W-V-Nb developed in Japan, is a low alloy heat resistant steel with good comprehensive properties. Influence of long term creep at elevated temperature on the structure of 2.25Cr-1.6W-V-Nb steel was studied in this paper, and the micromechanism of creep strength degradation was elucidated, too. Both TEM observation and thermodynamic calculation reveal that during creep the transformation occurs from M7C3 and M23C6 to M6C,which can be cavity nucleation sites. Besides, creep at 600℃ also leads to the decrease of dislocation density, the coarsening and coalescence of M23C6, the nucleation of cavities and development of cracks. The strength decrease of 2.25Cr-1.6W-V-Nb steel after long term creep is related to the decrease of dislocation hardening, precipitation hardening, solution hardening, the nucleation of cavities and development of cracks.

  12. Characterization and physiological role of two types of chloroplastic fructose-1,6-bisphosphatases in Euglena gracilis.

    Science.gov (United States)

    Ogawa, Takahisa; Kimura, Ayako; Sakuyama, Harumi; Tamoi, Masahiro; Ishikawa, Takahiro; Shigeoka, Shigeru

    2015-06-01

    The chloroplastic fructose-1,6-bisphosphatase (FBPase) is a late-limiting enzyme in the Calvin cycle. In the present study, we isolated and characterized the cDNAs encoding two types of chloroplastic FBPase isoforms (EgFBPaseI and II) from Euglena gracilis. The Km values of recombinant EgFBPaseI and EgFBPaseII for fructose 1,6-bisphosphate (Fru 1,6-P2) were 165 ± 17 and 2200 ± 200 μM, respectively. The activity of EgFBPaseI was inhibited by 1mM H2O2 and recovered when incubated with DTT. The activity of EgFBPaseII was resistant to concentrations of H2O2 up to 1mM, which was distinct from those of EgFBPaseI and spinach chloroplastic FBPase. The suppression of EgFBPaseI gene expression by gene silencing markedly decreased photosynthetic activity and inhibited cell growth. The results of the present study clearly demonstrated that EgFBPaseI played a critical role in photosynthesis in Euglena chloroplasts.

  13. Structure of tagatose-1,6-bisphosphate aldolase. Insight into chiral discrimination, mechanism, and specificity of class II aldolases.

    Science.gov (United States)

    Hall, David R; Bond, Charles S; Leonard, Gordon A; Watt, C Ian; Berry, Alan; Hunter, William N

    2002-06-14

    Tagatose-1,6-bisphosphate aldolase (TBPA) is a tetrameric class II aldolase that catalyzes the reversible condensation of dihydroxyacetone phosphate with glyceraldehyde 3-phosphate to produce tagatose 1,6-bisphosphate. The high resolution (1.45 A) crystal structure of the Escherichia coli enzyme, encoded by the agaY gene, complexed with phosphoglycolohydroxamate (PGH) has been determined. Two subunits comprise the asymmetric unit, and a crystallographic 2-fold axis generates the functional tetramer. A complex network of hydrogen bonds position side chains in the active site that is occupied by two cations. An unusual Na+ binding site is created using a pi interaction with Tyr183 in addition to five oxygen ligands. The catalytic Zn2+ is five-coordinate using three histidine nitrogens and two PGH oxygens. Comparisons of TBPA with the related fructose-1,6-bisphosphate aldolase (FBPA) identifies common features with implications for the mechanism. Because the major product of the condensation catalyzed by the enzymes differs in the chirality at a single position, models of FBPA and TBPA with their cognate bisphosphate products provide insight into chiral discrimination by these aldolases. The TBPA active site is more open on one side than FBPA, and this contributes to a less specific enzyme. The availability of more space and a wider range of aldehyde partners used by TBPA together with the highly specific nature of FBPA suggest that TBPA might be a preferred enzyme to modify for use in biotransformation chemistry.

  14. Single-molecule imaging of Nav1.6 on the somatic surface of hippocampal neurons reveals unique nanoclusters

    CERN Document Server

    Akin, Elizabeth J; Johnson, Ben; Beheiry, Mohamed el; Masson, Jean-Baptiste; Krapf, Diego; Tamkun, Michael M

    2016-01-01

    Voltage-gated sodium (Na$_v$) channels are responsible for the depolarizing phase of the action potential in most nerve cells and Na$_v$ channel localization to the axon initial segment is vital to action potential initiation. Na$_v$ channels in the soma play a role in the transfer of axonal output information to the rest of the neuron and in synaptic plasticity, although little is known about Na$_v$ channel localization and dynamics within this neuronal compartment. The present study uses single-particle tracking and photoactivation localization microscopy to analyze cell-surface Na$_v$1.6 within the soma of cultured hippocampal neurons. Mean square displacement analysis of individual trajectories indicated half of the somatic Na$_v$1.6 channels localized to stable nanoclusters ~230 nm in diameter. Strikingly, these domains were stabilized at specific sites on the cell membrane for greater than 30 min, notably via an ankyrin-independent mechanism, indicating the mechanism by which Na$_v$1.6 nanoclusters are ...

  15. Mutan: A mixed linkage α-[(1,3)- and (1,6)]-d-glucan from Streptococcus mutans, that induces osteoclast differentiation and promotes alveolar bone loss.

    Science.gov (United States)

    Kwon, Hyun-Jung; Kim, Jung Min; Han, Kook-Il; Jung, Eui-Gil; Kim, Yong Hyun; Patnaik, Bharat Bhusan; Yoon, Mi Sook; Chung, Sung Kyun; Kim, Wan Jong; Han, Man-Deuk

    2016-02-10

    Mutan is an extracellular polysaccharide of Streptococcus mutans (S. mutans) that consists of α-(1,3)-linked glucose residues in main chains and α-(1,6) bonds in side chains. In the present study, mutan was isolated from S. mutans, and its structural characteristics were determined using Fourier-transform infrared spectroscopy (FT-IR) and (13)C nuclear magnetic resonance (NMR) spectroscopy. The effects of mutan on RANKL-induced osteoclast differentiation in RAW 264.7 cells were examined. Furthermore, microCT and morphometric analyses were used to determine the contribution of mutan to alveolar bone loss in the maxilla of a rat periodontitis model. Mutan increased (more than 2-fold) RANKL-induced osteoclast differentiation in a dose-dependent manner. Mutan also enhanced the alveolar bone loss in the rat maxilla 2.3-fold. In mutan-treated rats, the bone mineral density, bone volume, trabecular number, and trabecular thickness decreased, whereas trabecular separation significantly increased. In addition, mutan and lipopolysaccharide (LPS) induced similar microarray profiles in RAW 264.7 cells. A total of 43 genes related to osteoclastogenesis were differentially expressed after either mutan or LPS treatment. Five-fold increases in the expression of several genes, including IL-1β, IL-1α, IL-6, and chemokine ligands, were observed in mutan-treated RAW 264.7 cells. These results suggest a molecular mechanism for the inflammation induced by S. mutans during the establishment of periodontal disease.

  16. Three-dimensional preservation of cellular and subcellular structures suggests 1.6 billion-year-old crown-group red algae

    Science.gov (United States)

    Bengtson, Stefan; Sallstedt, Therese; Belivanova, Veneta; Whitehouse, Martin

    2017-01-01

    The ~1.6 Ga Tirohan Dolomite of the Lower Vindhyan in central India contains phosphatized stromatolitic microbialites. We report from there uniquely well-preserved fossils interpreted as probable crown-group rhodophytes (red algae). The filamentous form Rafatazmia chitrakootensis n. gen, n. sp. has uniserial rows of large cells and grows through diffusely distributed septation. Each cell has a centrally suspended, conspicuous rhomboidal disk interpreted as a pyrenoid. The septa between the cells have central structures that may represent pit connections and pit plugs. Another filamentous form, Denaricion mendax n. gen., n. sp., has coin-like cells reminiscent of those in large sulfur-oxidizing bacteria but much more recalcitrant than the liquid-vacuole-filled cells of the latter. There are also resemblances with oscillatoriacean cyanobacteria, although cell volumes in the latter are much smaller. The wider affinities of Denaricion are uncertain. Ramathallus lobatus n. gen., n. sp. is a lobate sessile alga with pseudoparenchymatous thallus, “cell fountains,” and apical growth, suggesting florideophycean affinity. If these inferences are correct, Rafatazmia and Ramathallus represent crown-group multicellular rhodophytes, antedating the oldest previously accepted red alga in the fossil record by about 400 million years. PMID:28291791

  17. Structures of NodZ [alpha]1,6-fucosyltransferase in complex with GDP and GDP-fucose

    Energy Technology Data Exchange (ETDEWEB)

    Brzezinski, Krzysztof; Dauter, Zbigniew; Jaskolski, Mariusz (NCI); (Polish)

    2012-03-26

    Rhizobial NodZ {alpha}1,6-fucosyltransferase ({alpha}1,6-FucT) catalyzes the transfer of the fucose (Fuc) moiety from guanosine 5'-diphosphate-{beta}-L-fucose to the reducing end of the chitin oligosaccharide core during Nod-factor (NF) biosynthesis. NF is a key signaling molecule required for successful symbiosis with a legume host for atmospheric nitrogen fixation. To date, only two {alpha}1,6-FucT structures have been determined, both without any donor or acceptor molecule that could highlight the structural background of the catalytic mechanism. Here, the first crystal structures of {alpha}1,6-FucT in complex with its substrate GDP-Fuc and with GDP, which is a byproduct of the enzymatic reaction, are presented. The crystal of the complex with GDP-Fuc was obtained through soaking of native NodZ crystals with the ligand and its structure has been determined at 2.35 {angstrom} resolution. The fucose residue is exposed to solvent and is disordered. The enzyme-product complex crystal was obtained by cocrystallization with GDP and an acceptor molecule, penta-N-acetyl-L-glucosamine (penta-NAG). The structure has been determined at 1.98 {angstrom} resolution, showing that only the GDP molecule is present in the complex. In both structures the ligands are located in a cleft formed between the two domains of NodZ and extend towards the C-terminal domain, but their conformations differ significantly. The structures revealed that residues in three regions of the C-terminal domain, which are conserved among {alpha}1,2-, {alpha}1,6- and protein O-fucosyltransferases, are involved in interactions with the sugar-donor molecule. There is also an interaction with the side chain of Tyr45 in the N-terminal domain, which is very unusual for a GT-B-type glycosyltransferase. Only minor conformational changes of the protein backbone are observed upon ligand binding. The only exception is a movement of the loop located between strand {beta}C2 and helix {alpha}C3. In addition

  18. Overexpression of NaV 1.6 channels is associated with the invasion capacity of human cervical cancer.

    Science.gov (United States)

    Hernandez-Plata, Everardo; Ortiz, Cindy S; Marquina-Castillo, Brenda; Medina-Martinez, Ingrid; Alfaro, Ana; Berumen, Jaime; Rivera, Manuel; Gomora, Juan C

    2012-05-01

    Functional activity of voltage-gated sodium channels (VGSC) has been associated to the invasion and metastasis behaviors of prostate, breast and some other types of cancer. We previously reported the functional expression of VGSC in primary cultures and biopsies derived from cervical cancer (CaC). Here, we investigate the relative expression levels of VGSC subunits and its possible role in CaC. Quantitative real-time PCR revealed that mRNA levels of Na(V) 1.6 α-subunit in CaC samples were ∼40-fold higher than in noncancerous cervical (NCC) biopsies. A Na(V) 1.7 α-subunit variant also showed increased mRNA levels in CaC (∼20-fold). All four Na(V) β subunits were also detected in CaC samples, being Na(V) β1 the most abundant. Proteins of Na(V) 1.6 and Na(V) 1.7 α-subunits were immunolocalized in both NCC and CaC biopsies and in CaC primary cultures as well; however, although in NCC sections proteins were mainly relegated to the plasma membrane, in CaC biopsies and primary cultures the respective signal was stronger and widely distributed in both cytoplasm and plasma membrane. Functional activity of Na(V) 1.6 channels in the plasma membrane of CaC cells was confirmed by whole-cell patch-clamp experiments using Cn2, a Na(V) 1.6-specific toxin, which blocked ∼30% of the total sodium current. Blocking of sodium channels VGSC with tetrodotoxin and Cn2 did not affect proliferation neither migration, but reduced by ∼20% the invasiveness of CaC primary culture cells in vitro assays. We conclude that Na(V) 1.6 is upregulated in CaC and could serve as a novel molecular marker for the metastatic behavior of this carcinoma.

  19. Structures of NodZ α1,6-fucosyltransferase in complex with GDP and GDP-fucose.

    Science.gov (United States)

    Brzezinski, Krzysztof; Dauter, Zbigniew; Jaskolski, Mariusz

    2012-02-01

    Rhizobial NodZ α1,6-fucosyltransferase (α1,6-FucT) catalyzes the transfer of the fucose (Fuc) moiety from guanosine 5'-diphosphate-β-L-fucose to the reducing end of the chitin oligosaccharide core during Nod-factor (NF) biosynthesis. NF is a key signalling molecule required for successful symbiosis with a legume host for atmospheric nitrogen fixation. To date, only two α1,6-FucT structures have been determined, both without any donor or acceptor molecule that could highlight the structural background of the catalytic mechanism. Here, the first crystal structures of α1,6-FucT in complex with its substrate GDP-Fuc and with GDP, which is a byproduct of the enzymatic reaction, are presented. The crystal of the complex with GDP-Fuc was obtained through soaking of native NodZ crystals with the ligand and its structure has been determined at 2.35 Å resolution. The fucose residue is exposed to solvent and is disordered. The enzyme-product complex crystal was obtained by cocrystallization with GDP and an acceptor molecule, penta-N-acetyl-L-glucosamine (penta-NAG). The structure has been determined at 1.98 Å resolution, showing that only the GDP molecule is present in the complex. In both structures the ligands are located in a cleft formed between the two domains of NodZ and extend towards the C-terminal domain, but their conformations differ significantly. The structures revealed that residues in three regions of the C-terminal domain, which are conserved among α1,2-, α1,6- and protein O-fucosyltransferases, are involved in interactions with the sugar-donor molecule. There is also an interaction with the side chain of Tyr45 in the N-terminal domain, which is very unusual for a GT-B-type glycosyltransferase. Only minor conformational changes of the protein backbone are observed upon ligand binding. The only exception is a movement of the loop located between strand βC2 and helix αC3. In addition, there is a shift of the αC3 helix itself upon GDP

  20. 1,6-二磷酸果糖对蓝氏贾第鞭毛虫滋养体形态学的影响%Effect of fructose 1,6-biphosphate on morphology of Giardia lamblia trophozoites

    Institute of Scientific and Technical Information of China (English)

    冯宪敏; 鞠晓红; 王月华; 朱枫; 藏秋雨; 时文艳; 卢思奇

    2012-01-01

    Objective To study the effect of fructose 1,6-biphosphate ( FBP ) on the morphology of Giarida lamblia trophozoites. Methods Fructose 1,6-diphosphate was added into each cultural tube with the final concentration of 30 ng/μ1 immediately for group A and 4 hours later for group B after the inoculation at concentration of 0. 5 × 10 /ml. The adherence, activity and morphology of trophozoites were detected under the inverted microscope at 24 h, 48 h, 72 h and 96 h after drug administration, respectively. Trophozoites in each group were collected at those time points. The concentrations of trophozoites were calculated and the growth curves were constructed. Meanwhile, a group cultured normally was set as a control( group C ). Results Compared with group C, morphology of the trophozoites changed significantly with activity and adherence of trophozoites decreasing at the time point of 48 h after exposing to FBP. No adherent trophozoites were found after 72 h. All the trophozoites died after 96 h. The growth curve showed that the growth of trophozoites was considerably depressed after 24 h exposing to FBP. Half of the trophozoites died after 48 h and no alive ones were detected after 96 h. The difference were statistically significant between group A, B and C ( P < 0. 01 ). Conclusions Over accumulation of fructose 1, 6-biphosphate can induce the morphology damage and death of Giardia lamblia trophozoites. But, further study of the mechanism should be performed.%目的 探讨1,6-二磷酸果糖对体外培养的蓝氏贾第鞭毛虫(贾第虫)滋养体形态学的影响.方法 按0.5 × 106/ml的贾第虫滋养体浓度接种于4 ml培养瓶,接种后即时(A组)或接种后4 h(B组)加入1,6-二磷酸果糖,终浓度30 ng/μl.分别于药物作用后24、48、72和96 h以倒置显微镜观察虫体的贴壁情况、活力和形态变化;收集各组、各时段虫体,计数虫体滋养体浓度并绘制虫体生长曲线;同时,设立正常培养

  1. Cost of US health reforms is estimated at $1.6 trillion for first 10 years%美国医疗改革最初10年预计花费1.6万亿美元

    Institute of Scientific and Technical Information of China (English)

    Bob Roehr; 齐文安

    2009-01-01

    @@ 根据美国国会预算办公室的估算,Barack Obama总统所提议的医疗改革计划在最初10年需新增款项达1.6万亿美元(约合1万亿英镑;1.1万亿欧元).这一金额比其拥护者预期的花费至少超出1/3.

  2. Study on the fermenting technology of Fructose-1,6-diphosphate with brewer's yeast%啤酒废酵母制备1,6-二磷酸果糖的研究

    Institute of Scientific and Technical Information of China (English)

    王迎辉; 卢晓霆; 常启龙

    2008-01-01

    啤酒废酵母生产1,6-二磷酸果糖发酵工艺进行了试验性探讨.正交试验结果表明,啤酒废酵母发酵生产FDP的最佳工艺参数为:发酵体系中葡萄糖的加量为0.5 mol/L、无机磷的浓度为0.4 mol/L、pH 6.5、转化时间6 h.

  3. The crystal structure of Cu1.6Pb1.6Bi6.4S12, a new 44.8 Å derivative of the bismuthinite-aikinite solid-solution series

    DEFF Research Database (Denmark)

    Topa, Dan; Balic-Zunic, Tonci; Makovicky, Emil

    2000-01-01

    Cu1.6Pb1.6Bi6.4S12, aikinite-bismuthinite derivative, crystal structure, Felbertal, scheelite deposit, Austria......Cu1.6Pb1.6Bi6.4S12, aikinite-bismuthinite derivative, crystal structure, Felbertal, scheelite deposit, Austria...

  4. 1,6 two phosphate fructose treatment viral myocarditis clinical observation%1,6二磷酸果糖治疗病毒性心肌炎临床观察

    Institute of Scientific and Technical Information of China (English)

    张玉清

    2011-01-01

    Objective To explore 1 ,6 fructose diphosphate intravenous dripping of the treatment for viral myocarditis the clinical effect. Methods To select 60 fire viral myocarditis cases which accept the treatment in our hospital from 2006 January to 2009 July. Then randomly divide them into tow groups : one is for treatment and the other one is for comparison. The two groups have the same foundation treatment. And the 35 examples in the treatment group use 1,6 phosphoric acid fructose 100 ~ 250mg ( kg . d) intravenous dripping note. 25 patients in compared group was added Vitcl00 ~ 200mg/( kg· d) intravenous infusion,for 14 days treatment. Results For two weeks later,94% of the patients ' symptoms completely disappeared (33/35) . and in the compared group is 84% (21/25) (P < 0. 01) . After the treatment. the ECG of the patients changed. In all the the patients, the proportion of whose symptoms had resume in the treatment group is 91% (32/35 ) . While in the compared group is 56% ( 14/25) ( P < 0. 05 ) . Two weeks later both of the two groups came back to nomal. 56% ( 9/16) ( P < 0. 05 ) of the ventricular premature pulse group come back to normal in two weeks. myocardium enzyme test: in the 2nd week, CK-MB , cTnT. the recoverd is 83% .77% in all, and 50% ,52% come hack to normal( P1 < 0. 05 ,P2 < 0. 01) ,in 2 ~ 3 months, there is few differences between the normal mvocardium enzyme and ahnormal myocardium enzyme( P > 0. 05 ) . Conclusion About the viral myocarditis , using 1 ,6fructose diphosphate can short the course of disease than using large dose Vitc ,from the situation of the clinlcal symptom , physical sign. electrocardiogram, myocardium enzyme, 1 ,6 fructose diphosphate is the effective way to cure myocarditis%目的 探讨1,6二磷酸果糖静脉滴注治疗病毒性心肌炎的临床疗效.方法 选择我院2006年1月~2009年7月收治的病毒性心肌炎患儿60例,随机分为治疗组及对照组,两

  5. Three exopolysaccharides of the beta-(1-->6)-D-glucan type and a beta-(1-->3;1-->6)-D-glucan produced by strains of Botryosphaeria rhodina isolated from rotting tropical fruit.

    Science.gov (United States)

    Vasconcelos, Ana Flora D; Monteiro, Nilson K; Dekker, Robert F H; Barbosa, Aneli M; Carbonero, Elaine R; Silveira, Joana L M; Sassaki, Guilherme L; da Silva, Roberto; de Lourdes Corradi da Silva, Maria

    2008-09-22

    Four exopolysaccharides (EPS) obtained from Botryosphaeria rhodina strains isolated from rotting tropical fruit (graviola, mango, pinha, and orange) grown on sucrose were purified on Sepharose CL-4B. Total acid hydrolysis of each EPS yielded only glucose. Data from methylation analysis and (13)C NMR spectroscopy indicated that the EPS from the graviola isolate consisted of a main chain of glucopyranosyl (1-->3) linkages substituted at O-6 as shown in the putative structure below: [carbohydrate structure: see text]. The EPS of the other fungal isolates consisted of a linear chain of (1-->6)-linked glucopyranosyl residues of the following structure: [carbohydrate structure: see text]. FTIR spectra showed one band at 891 cm(-1), and (13)C NMR spectroscopy showed that all glucosidic linkages were of the beta-configuration. Dye-inclusion studies with Congo Red indicated that each EPS existed in a triple-helix conformational state. beta-(1-->6)-d-Glucans produced as exocellular polysaccharides by fungi are uncommon.

  6. A Comparative Study of Constitutive and Neural Network Models for Flow Behavior of Mg-5.9Zn-1.6Zr-1.6Nd-0.9Y Alloy and Processing Maps

    Science.gov (United States)

    Yan, Liangming; An, Di; Shi, Ge; Bian, Mingzhe; Khanra, Asit Kumar; Wang, Huiting

    2017-05-01

    Isothermal and constant strain rate compression tests of as-homogenized Mg-5.9Zn-1.6Zr-1.6Nd-0.9Y alloy were performed by using Gleeble-3800 thermo-mechanical simulator at temperature between 523 and 723 K and strain rate range from 0.001 to 1 s-1, respectively. The deformation behavior of the alloy at elevated temperature was investigated. A strain-dependent constitutive model based on the Arrhenius model and back-propagation neural network (BPNN) model of the flow stress was established. The predictability of the two models was evaluated by average absolute relative error (AARE) and correlation coefficient ( R c), respectively. The results show that the BPNN model has more accurate predictability than strain-dependent constitutive model. Processing maps are obtained according to the principles of the dynamic materials modeling and microstructures of the compressed samples. The microstructure of the samples was observed by optical microscope and electron backscattered diffraction. Processing maps show that the instability domain is at a strain rate of range of 0.015-1 s-1 and a temperature between 523 and 623 K. The alloy has good workability at 630-700 K and 0.008-0.1 s-1, wherein dynamic recrystallization occurs.

  7. Immunochemical studies on the N-acetyllactosamine beta-(1----6)-linked trisaccharide specificity of Ricinus communis agglutinin.

    Science.gov (United States)

    Wu, A M; Sugii, S; Gruezo, F G; Kabat, E A

    1988-07-15

    The combining site of Ricinus communis agglutinin (RCA1) was studied by quantitative precipitin and precipitin inhibition assays. Of 31 complex carbohydrates tested, all except active and inactive antifreeze glycoproteins, Streptococcus group C polysaccharide, and native rat salivary glycoprotein, reacted strongly, and 22 completely precipitated the lectin, indicating that RCA1 has both a broad range of affinity and a low solubility of its carbohydrate-bound complex. Of the monosaccharides and glycosides tested for inhibition of precipitation, p-nitrophenyl beta-D-galactopyranoside was the best. It was about 6.4 times better than methyl beta-D-galactopyranoside. The beta anomer of glycosides of D-galactose was much more potent than the corresponding alpha anomer. Among the oligosaccharides tested, beta-D-Galp-(1----4)-beta-D-GlcpNAc-(1----6)-D-Gal was the best inhibitor, which was approximately 2/3 as active as p-nitrophenyl beta-D-galactopyranoside. It was approximately 1.4 times as active as beta-D-Gal-(1----4)-D-GlcNAc (N-acetyllactosamine), twice as active as beta-D-Gal-(1----3)-D-GlcNAc, and 4.5 times more active than lacto-N-tetraose. From the results, it can be concluded that; (a) hydrophobic interaction is important for binding; (b) the combining site of this lectin is at least as large as a trisaccharide; and (c) of the compounds studied, the trisaccharide beta-D-Galp-(1----4)-beta-D-GlcpNAc-(1----6)-D-Gal was the most complementary to the human blood group I Ma determinant beta-D-Galp-(1----4)-beta-D-GlcpNAc-(1----6)-D-Gal.

  8. The new 'Earth Dreams Technology i-DTEC' 1.6 l diesel engine from Honda

    Energy Technology Data Exchange (ETDEWEB)

    Yamano, J.; Ikoma, K.; Matsui, R.; Ikegami, N.; Mori, S.; Yano, T. [Honda R and D Co., Ltd., Tochigi (Japan)

    2013-08-01

    Honda has developed a 3rd-generation diesel engine, seeking to balance further CO{sub 2} reductions with dynamic performance. This development focused on downsizing the engine and succeeded in developing a compact, lightweight and high-efficiency 1.6 L in-line 4-cylinder turbocharged i-DTEC diesel engine. Optimization of engine rigidity in the newly developed 1.6 L diesel engine has made it possible to use an aluminum cylinder block with an open-deck structure. Furthermore, weight could be reduced by means of an efficient structure and engine layout. In addition, mechanical friction has been minimized via reducing weight of the reciprocating components and downsizing auxiliary equipment. These innovations made it possible for the engine to achieve the same level of friction as a Honda petrol engine of the same displacement. Thermal management has also been optimized by enhancement of the engine cooling system. In addition, low-pressure loop exhaust gas recirculation (LP-EGR) was applied to achieve increased thermal efficiency. These measures have helped the engine to realize a high level of boost and high EGR, increasing fuel efficiency and reducing emissions across a wide range of operating conditions. Like the 2.2 L model, the Civic fitted with this 1.6 L diesel engine uses idle-stop and deceleration energy regeneration control. With all these measures, the Civic achieved CO{sub 2} emissions of 94 g/km (3.6 L/100km) in NEDC, a reduction of 14.5% in CO{sub 2} emissions against the 110 g/km recorded by the 2.2 L model. (orig.)

  9. Hombre, bienaventuranza y Dios en Suma Teológica l-ll, qq. 1-6.

    Directory of Open Access Journals (Sweden)

    Ariadne Pérez Treviño

    2013-11-01

    Full Text Available This article studies the Aquinas' proposal on Summa Theologica I-II, qq-1-6, where he studies how should the final end of the man be and concludes that it should be transcendental in a certain way, but immanent in another and that God is the immanent end; then, he studies the way in which man will get it and he finds out that it requires unity in three ways: unity between body and soul, intelligence and will, and finally, between the man, who is well disposed to contemplation and contemplation itself.

  10. Purification and amino acid sequence of fructose-1,6-bisphosphate aldolase from the electric organ of Electrophorus electricus (L.).

    Science.gov (United States)

    De-Simone, Salvatore G; de Salles, Christiane M Cardoso; Batista e Silva, Celia M; Hassón-Voloch, Aida

    2006-01-01

    A soluble fructose-1,6-bisphosphate aldolase enzyme has been purified 50.2-fold (2.36%) at the homogeneity from the electric organ of Electrophorus electricus by one step of DEAE-52 anion exchange chromatography followed by Superose-12 gel filtration-FPLC. Like other aldolase enzymes the E. electricus protein is a dimer with two identical subunits of 45 kDa. The N-terminal (20 residues) revealed a high homology with S. aurata (75%, goldfish), R. ratus and M. musculus (mouse, 80%) enzymes.

  11. Stereoengineering of poly(1,3-methylenecyclohexane) via two-state living coordination polymerization of 1,6-heptadiene.

    Science.gov (United States)

    Crawford, Kaitlyn E; Sita, Lawrence R

    2013-06-19

    External control over the rate of dynamic methyl group exchange between configurationally stable active species and configurationally unstable dormant species with respect to chain-growth propagation within a highly stereoselective and regiospecific living coordination polymerization of 1,6-heptadiene has been used to generate a spectrum of different physical forms of poly(1,3-methylenecyclohexane) (PMCH) in which the stereochemical microstructure has been systematically varied between two limiting forms. The application of this strategy to manipulate the bulk properties of PMCH and the solid-state microphase behavior of well-defined PMCH-b-poly(1-hexene) block copolymers is further demonstrated.

  12. Aberrant epilepsy-associated mutant Nav1.6 sodium channel activity can be targeted with cannabidiol.

    Science.gov (United States)

    Patel, Reesha R; Barbosa, Cindy; Brustovetsky, Tatiana; Brustovetsky, Nickolay; Cummins, Theodore R

    2016-08-01

    Mutations in brain isoforms of voltage-gated sodium channels have been identified in patients with distinct epileptic phenotypes. Clinically, these patients often do not respond well to classic anti-epileptics and many remain refractory to treatment. Exogenous as well as endogenous cannabinoids have been shown to target voltage-gated sodium channels and cannabidiol has recently received attention for its potential efficacy in the treatment of childhood epilepsies. In this study, we further investigated the ability of cannabinoids to modulate sodium currents from wild-type and epilepsy-associated mutant voltage-gated sodium channels. We first determined the biophysical consequences of epilepsy-associated missense mutations in both Nav1.1 (arginine 1648 to histidine and asparagine 1788 to lysine) and Nav1.6 (asparagine 1768 to aspartic acid and leucine 1331 to valine) by obtaining whole-cell patch clamp recordings in human embryonic kidney 293T cells with 200 μM Navβ4 peptide in the pipette solution to induce resurgent sodium currents. Resurgent sodium current is an atypical near threshold current predicted to increase neuronal excitability and has been implicated in multiple disorders of excitability. We found that both mutations in Nav1.6 dramatically increased resurgent currents while mutations in Nav1.1 did not. We then examined the effects of anandamide and cannabidiol on peak transient and resurgent currents from wild-type and mutant channels. Interestingly, we found that cannabidiol can preferentially target resurgent sodium currents over peak transient currents generated by wild-type Nav1.6 as well as the aberrant resurgent and persistent current generated by Nav1.6 mutant channels. To further validate our findings, we examined the effects of cannabidiol on endogenous sodium currents from striatal neurons, and similarly we found an inhibition of resurgent and persistent current by cannabidiol. Moreover, current clamp recordings show that cannabidiol reduces

  13. Sub-90 fs dissipative-soliton Erbium-doped fiber lasers operating at 1.6 μm band.

    Science.gov (United States)

    Wang, Zhiqiang; Qian, Kai; Fang, Xiao; Gao, Caixia; Luo, Hao; Zhan, Li

    2016-05-16

    We present an L-band dissipative soliton (DS) fiber laser, which can deliver 87.5 fs pulses at 1.6 μm band. Numerical simulations are used to confirm the DS generation, and prove the pivotal component of the invisible filter with proper bandwidth in the formation of DS pulses. Such a robust, compact ultrafast laser source with higher pulse energy is hence an excellent seed source for L-band amplifiers. The mechanism revealed in the simulations is helpful to develop a unified theory for understanding various mode-locking regimes in normal dispersion lasers.

  14. Organocatalytic Domino Oxa-Michael/1,6-Addition Reactions: Asymmetric Synthesis of Chromans Bearing Oxindole Scaffolds.

    Science.gov (United States)

    Zhao, Kun; Zhi, Ying; Shu, Tao; Valkonen, Arto; Rissanen, Kari; Enders, Dieter

    2016-09-19

    An asymmetric organocatalytic domino oxa-Michael/1,6-addition reaction of ortho-hydroxyphenyl-substituted para-quinone methides and isatin-derived enoates has been developed. In the presence of 5 mol % of a bifunctional thiourea organocatalyst, this scalable domino reaction affords 4-phenyl-substituted chromans bearing spiro-connected oxindole scaffolds and three adjacent stereogenic centers in good to excellent yields (up to 98 %) and with very high stereoselectivities (up to >20:1 d.r., >99 % ee). © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  15. Free ion yields for nonpolar liquids exposed to 1.6-3.5 keV X-rays

    CERN Document Server

    Holroyd, R A

    1998-01-01

    The yields of free ions formed following absorption of 1.6-3.5 keV X-rays were determined for several nonpolar liquids using a conductivity technique. The yields are much less for X-rays than for gamma rays; this effect is largest for branched hydrocarbons. A minimum in yield is observed around 2 keV. The dependence of G sub f sub i sup o on X-ray energy is in good agreement with computer simulations. For tetramethylsilane a sharp dip in ion yield is observed at the Si ls -> sigma sup * resonance, indicating that the free electron yield is even less at this energy.

  16. Bone loss during partial weight bearing (1/6th gravity) is mitigated by resistance and aerobic exercise in mice

    Science.gov (United States)

    Boudreaux, R. D.; Metzger, C. E.; Macias, B. R.; Shirazi-Fard, Y.; Hogan, H. A.; Bloomfield, S. A.

    2014-06-01

    Astronauts on long duration missions continue to experience bone loss, as much as 1-2% each month, for up to 4.5 years after a mission. Mechanical loading of bone with exercise has been shown to increase bone formation, mass, and geometry. The aim of this study was to compare the efficacy of two exercise protocols during a period of reduced gravitational loading (1/6th body weight) in mice. Since muscle contractions via resistance exercise impart the largest physiological loads on the skeleton, we hypothesized that resistance training (via vertical tower climbing) would better protect against the deleterious musculoskeletal effects of reduced gravitational weight bearing when compared to endurance exercise (treadmill running). Young adult female BALB/cBYJ mice were randomly assigned to three groups: 1/6 g (G/6; n=6), 1/6 g with treadmill running (G/6+RUN; n=8), or 1/6 g with vertical tower climbing (G/6+CLB; n=9). Exercise was performed five times per week. Reduced weight bearing for 21 days was achieved through a novel harness suspension system. Treadmill velocity (12-20 m/min) and daily run time duration (32-51 min) increased incrementally throughout the study. Bone geometry and volumetric bone mineral density (vBMD) at proximal metaphysis and mid-diaphysis tibia were assessed by in vivo peripheral quantitative computed tomography (pQCT) on days 0 and 21 and standard dynamic histomorphometry was performed on undemineralized sections of the mid-diaphysis after tissue harvest. G/6 caused a significant decrease (P<0.001) in proximal tibia metaphysis total vBMD (-9.6%). These reductions of tibia metaphyseal vBMD in G/6 mice were mitigated in both G/6+RUN and G/6+CLB groups (P<0.05). After 21 days of G/6, we saw an absolute increase in tibia mid-diaphysis vBMD and in distal metaphysis femur vBMD in both G/6+RUN and G/6+CLB mice (P<0.05). Substantial increases in endocortical and periosteal mineralizing surface (MS/BS) at mid-diaphysis tibia in G/6+CLB demonstrate that

  17. A New Orthorhombic Phase of ZrW1.6Mo0.4O8 at Ambient Pressure

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Nanocrystallite δ′-ZrW1.6Mo0.4O8 was prepared in the precursor route synthesis. The characterization by means of powder X-ray diffraction (XRD) and selected area electron diffraction (SAED) showed it crystallized in a orthorhombic crystal system with a=0.896 9(7) nm, b=0.701 1(8) nm, c=0.596(1) nm. The possible space group is Pnnm (58) or Pnn2 (34). The compound crystallizes in a metastable phase during the synthesis process depending on temperature and crystallization time.

  18. 1,6-二氯二苯并-对-二噁英的合成与表征%Synthesis and characterization of 1,6-dichlorodibenzyl para-dioxin

    Institute of Scientific and Technical Information of China (English)

    李敬; 张学良; 郝双红; 孙家隆

    2011-01-01

    As a kind of special fat-soluble organic compounds with high toxicity, dioxin is severely harmful to environment and human health.Besides, 1,6-dichloro-dibenzyl para-dioxin as a precursor of dioxin often exists in some pesticides, and its content in pesticides usually needs to be monitored before putting into use.Thus pyrocatechol was chlorinated with sulfonyl chloride yielding intermediate 4-chloro-pyrocatechol; then 4-chloro-pyrocatechol was allowed to react with 2,6-dichloro-nitrobenzene to give the title compound.The purity of the recrystalized final product was determined by means of high performance liquid chromatography and its structure was confirmed by nuclear magnetic resonance (1H NMR and 13C NMR) spectroscopy.Results indicate that the target product has a purity of 99.76%.%二噁英是一类毒性极大的特殊脂溶性有机化合物,严重污染环境和危害人类健康.1,6-二氯二苯并-对-二噁英是一种二噁英的前驱体,常作为杂质存在于某些农药产品中,在农药使用前需检测其含量.为此,采用磺酰氯氯化邻苯二酚得到中间产物4-氯邻苯二酚,再将中间产物与2,6-二氯硝基苯反应得到1,6-二氯二苯并-对-二噁英.利用高效液相色谱分析了产物的纯度,并通过气相色谱、核磁共振谱(1H NMR和13C NMR)对合成的目标物进行了表征.结果表明,目标产物柱层析品的纯度为99.76%.

  19. Evidence for strong evolution in galaxy environmental quenching efficiency between z = 1.6 and z = 0.9

    Science.gov (United States)

    Nantais, Julie B.; Muzzin, Adam; van der Burg, Remco F. J.; Wilson, Gillian; Lidman, Chris; Foltz, Ryan; DeGroot, Andrew; Noble, Allison; Cooper, Michael C.; Demarco, Ricardo

    2017-02-01

    We analyse the evolution of environmental quenching efficiency, the fraction of quenched cluster galaxies which would be star forming if they were in the field, as a function of redshift in 14 spectroscopically confirmed galaxy clusters with 0.87 < z < 1.63 from the Spitzer Adaptation of the Red-Sequence Cluster Survey. The clusters are the richest in the survey at each redshift. Passive fractions rise from 42_{-13}^{+10} per cent at z ˜ 1.6 to 80_{-9}^{+12} per cent at z ˜ 1.3 and 88_{-3}^{+4} per cent at z < 1.1, outpacing the change in passive fraction in the field. Environmental quenching efficiency rises dramatically from 16_{-19}^{+15} per cent at z ˜ 1.6 to 62_{-15}^{+21} per cent at z ˜ 1.3 and 73_{-7}^{+8} per cent at z ≲ 1.1. This work is the first to show direct observational evidence for a rapid increase in the strength of environmental quenching in galaxy clusters at z ˜ 1.5, where simulations show cluster-mass haloes undergo non-linear collapse and virialization.

  20. Intrinsic hepatocyte dedifferentiation is accompanied by upregulation of mesenchymal markers, protein sialylation and core alpha 1,6 linked fucosylation

    Science.gov (United States)

    Mehta, Anand; Comunale, Mary Ann; Rawat, Siddhartha; Casciano, Jessica C.; Lamontagne, Jason; Herrera, Harmin; Ramanathan, Aarti; Betesh, Lucy; Wang, Mengjun; Norton, Pamela; Steel, Laura F.; Bouchard, Michael J.

    2016-01-01

    Alterations in N-linked glycosylation have long been associated with cancer but for the most part, the reasons why have remained poorly understood. Here we show that increased core fucosylation is associated with de-differentiation of primary hepatocytes and with the appearance of markers indicative of a transition of cells from an epithelial to a mesenchymal state. This increase in core fucosylation was associated with increased levels of two enzymes involved in α-1,6 linked fucosylation, GDP-mannose 4, 6-dehydratase (Gmds) and to a lesser extent fucosyltransferase 8 (Fut8). In addition, the activation of cancer-associated cellular signaling pathways in primary rat hepatocytes can increase core fucosylation and induce additional glycoform alterations on hepatocyte proteins. Specifically, we show that increased levels of protein sialylation and α-1,6-linked core fucosylation are observed following activation of the β-catenin pathway. Activation of the Akt signaling pathway or induction of hypoxia also results in increased levels of fucosylation and sialylation. We believe that this knowledge will help in the better understanding of the genetic factors associated with altered glycosylation and may allow for the development of more clinically relevant biomarkers. PMID:27328854

  1. Automated docking of {alpha}-(1,4)- and {alpha}-(1,6)-linked glucosyl trisaccharides in the glucoamylase active site

    Energy Technology Data Exchange (ETDEWEB)

    Countinho, P.M.; Reilly, P.J. [Iowa State Univ., Ames, IA (United States). Dept. of Chemical Engineering; Dowd, M.K. [Dept. of Agriculture, New Orleans, LA (United States). Southern Regional Research Center

    1998-06-01

    Low-energy conformers of five {alpha}-(1,4)- and {alpha}-(1,6)-linked glucosyl trisaccharides were flexibly docked into the glucoamylase active site using AutoDock 2.2. To ensure that all significant conformational space was searched, the starting trisaccharide conformers for docking were all possible combinations of the corresponding disaccharide low-energy conformers. All docked trisaccharides occupied subsites {minus}1 and +1 in very similar modes to those of corresponding nonreducing-end disaccharides. For linear substrates, full binding at subsite +2 occurred only when the substrate reducing end was {alpha}-(1,4)-linked, with hydrogen-bonding with the hydroxy-methyl group being the only polar interaction there. Given the absence of other important interactions at this subsite, multiple substrate conformations are allowed. For the one docked branched substrate, steric hindrance in the {alpha}-(1,6)-glycosidic oxygen suggests that the active-site residues have to change position for hydrolysis to occur. Subsite +1 of the glucoamylase active site allows flexibility in binding but, at least in Aspergillus glucoamylases, subsite +2 selectively binds substrates {alpha}-(1,4)-linked between subsites +1 and +2. Enzyme engineering to limit substrate flexibility at subsite +2 could improve glucoamylase industrial properties.

  2. Separate effects testing and analyses to investigate liner tearing of the 1:6-scale reinforced concrete containment building

    Energy Technology Data Exchange (ETDEWEB)

    Spletzer, B.L.; Lambert, L.D.; Bergman, V.L. [Sandia National Labs., Albuquerque, NM (United States)] [and others

    1995-06-01

    The overpressurization of a 1:6-scale reinforced concrete containment building demonstrated that liner tearing is a plausible failure mode in such structures under severe accident conditions. A combined experimental and analytical program was developed to determine the important parameters which affect liner tearing and to develop reasonably simple analytical methods for predicting when tearing will occur. Three sets of test specimens were designed to allow individual control over and investigation of the mechanisms believed to be important in causing failure of the liner plate. The series of tests investigated the effect on liner tearing produced by the anchorage system, the loading conditions, and the transition in thickness from the liner to the insert plate. Before testing, the specimens were analyzed using two- and three-dimensional finite element models. Based on the analysis, the failure mode and corresponding load conditions were predicted for each specimen. Test data and post-test examination of test specimens show mixed agreement with the analytical predictions with regard to failure mode and specimen response for most tests. Many similarities were also observed between the response of the liner in the 1:6-scale reinforced concrete containment model and the response of the test specimens. This work illustrates the fact that the failure mechanism of a reinforced concrete containment building can be greatly influenced by details of liner and anchorage system design. Further, it significantly increases the understanding of containment building response under severe conditions.

  3. Evidence for strong evolution in galaxy environmental quenching efficiency between z = 1.6 and z = 0.9

    CERN Document Server

    Nantais, Julie B; van der Burg, Remco F J; Wilson, Gillian; Lidman, Chris; Foltz, Ryan; DeGroot, Andrew; Noble, Allison; Cooper, Michael C; Demarco, Ricardo

    2016-01-01

    We analyse the evolution of environmental quenching efficiency, the fraction of quenched cluster galaxies that would be star-forming if they were in the field, as a function of redshift in 14 spectroscopically confirmed galaxy clusters with 0.87 < z < 1.63 from the Spitzer Adaptation of the Red-Sequence Cluster Survey (SpARCS). The clusters are the richest in the survey at each redshift. Passive fractions rise from $42_{-13}^{+10}$\\% at z ~ 1.6 to $80_{-9}^{+12}$\\% at z ~ 1.3 and $88_{-3}^{+4}$\\% at z < 1.1, outpacing the change in passive fraction in the field. Environmental quenching efficiency rises dramatically from $16_{-19}^{+15}$ at z ~ 1.6 to $62_{-15}^{+21}\\% at z ~ 1.3 and $73_{-7}^{+8}$\\% at z $\\lesssim$ 1.1. This work is the first to show direct observational evidence for a rapid increase in the strength of environmental quenching in galaxy clusters at z ~ 1.5, where simulations show cluster-mass halos undergo non-linear collapse and virialisation.

  4. A 1.6-Mb P1-based physical map of the Down syndrome region on chromosome 21

    Energy Technology Data Exchange (ETDEWEB)

    Ohira, Miki; Suzuki, Kazunobu [National Cancer Center Research Institute, Tokyo (Japan)]|[Kazusa DNA Research Institute, Chiba (Japan); Ichikawa, Hitoshi [National Cancer Center Research Institute, Tokyo (Japan)] [and others

    1996-04-01

    The Down Syndrome (DS) region on chromosome 21, which is responsible for the main features of DS such as characteristic facial features, a congenital heart defect, and mental retardation, has been defined by molecular analysis of DS patients with partial trisomy 21. The 2.5-Mb region around the marker D21S55 between D21S17 and ERG in 21q22 is thought to be important, although contributions of other regions cannot be excluded. In this region, we focused on a 1.6-Mb region between a NotI site, LA68 (D21S396, which is mapped distal to D21S17) and ERG, because analysis of a Japanese DS family with partial trisomy 21 revealed that the proximal border of its triplicated region was distal to LA68. We constructed P1 contigs with 46 P1 clones covering more than 95% of the 1.6-Mb region. A high-resolution restriction map using BamHI was also constructed for more details analysis. Our P1 contig map supplements other physical maps previously reported and provides useful materials for further analysis including isolation and sequencing of the DS region. 31 refs., 7 figs., 1 tab.

  5. Partial branching enzyme treatment increases the low glycaemic property and α-1,6 branching ratio of maize starch.

    Science.gov (United States)

    Li, Xingfeng; Miao, Ming; Jiang, Huan; Xue, Jiangchao; Jiang, Bo; Zhang, Tao; Gao, Yaqi; Jia, Yingmin

    2014-12-01

    Partial branching enzyme treatment was used to modulate the starch fine chain structure responsible for a high content of slowly digestible starch fraction. Normal maize starch modified using branching enzyme for 4h showed a maximum slowly digestible starch content of 23.90%. The branching enzyme hydrolysis decreased the amylose content from 32.8% to 12.8%. The molecular weight distribution of enzyme-treated starches showed a larger proportion of low molecular weight fractions appeared in the enzyme treated starch sample compare to native starch. The number of shorter chains (DP30) from 20.11% to 11.95%. (1)H NMR spectra showed an increase of α-1,6 branching ratio from 4.7% to 9.4% during enzyme treatment. The increase in the amount of shorter chains and more α-1,6 linkages likely contribute to their slow digestion property. These results suggest that starches treated with partial branching enzyme synthesis a novel branched structure with slowly digestible character.

  6. Elucidation of new condition-dependent roles for fructose-1,6-bisphosphatase linked to cofactor balances.

    Science.gov (United States)

    Schabort, Du Toit W P; Kilian, Stephanus G; du Preez, James C

    2017-01-01

    The cofactor balances in metabolism is of paramount importance in the design of a metabolic engineering strategy and understanding the regulation of metabolism in general. ATP, NAD+ and NADP+ balances are central players linking the various fluxes in central metabolism as well as biomass formation. NADP+ is especially important in the metabolic engineering of yeasts for xylose fermentation, since NADPH is required by most yeasts in the initial step of xylose utilisation, including the fast-growing Kluyveromyces marxianus. In this simulation study of yeast metabolism, the complex interplay between these cofactors was investigated; in particular, how they may affect the possible roles of fructose-1,6-bisphosphatase, the pentose phosphate pathway, glycerol production and the pyruvate dehydrogenase bypass. Using flux balance analysis, it was found that the potential role of fructose-1,6-bisphosphatase was highly dependent on the cofactor specificity of the oxidative pentose phosphate pathway and on the carbon source. Additionally, the excessive production of ATP under certain conditions might be involved in some of the phenomena observed, which may have been overlooked to date. Based on these findings, a strategy is proposed for the metabolic engineering of a future xylose-fermenting yeast for biofuel production.

  7. Evolution of the Nuclear and Magnetic Structures with Temperature in TlFe1:6Se2

    Energy Technology Data Exchange (ETDEWEB)

    Cao, Huibo [ORNL; Chakoumakos, Bryan C [ORNL; May, Andrew F [ORNL; McGuire, Michael A [ORNL; Cantoni, Claudia [ORNL; Custelcean, Radu [ORNL; Sefat, A. S. [Oak Ridge National Laboratory (ORNL); Sales, Brian C [ORNL; Pennycook, Stephen J [ORNL

    2012-01-01

    The evolution of the nuclear and magnetic structures of TlFe1:6Se2 was determined in the temperature range of 5-450K using single crystal neutron diraction. The Fe layers in these materials develop a corrugation in the magnetically ordered state. A canting away from the block checkerboard magnetic structure is observed in the narrow temperature range between approximately 100 and 150 K. In this same temperature range, an increase in the corrugation of the Fe layers is observed. At lower temperatures, the block checkerboard magnetic structure is recovered with a suppressed magnetic moment and abrupt changes in the lattice parameters. Microstructure analysis at 300K using atomic-resolution Z-contrast scanning transmission electron microscopy does not show the clear phase separation observed in alkali-metal iron selenides. These ndings highlight the dierences between the alkali-metal and thallium materials, and indicate competition between magnetic ground states and a strong coupling of magnetism to the lattice in TlFe1:6Se2.

  8. Direct Detection 1.6?m DIAL / Doppler Lidar for Measurements of CO2 Concentration and Wind Profiles (Invited)

    Science.gov (United States)

    Shibata, Y.; Nagasawa, C.; Abo, M.

    2013-12-01

    Knowledge of present carbon sources and sinks including their spatial distribution and their variation in time is one of the essential information for predicting future CO2 atmospheric concentration levels. Moreover, wind information is an important parameter for transport simulations and inverse estimation of surface CO2 flux. The differential absorption lidar (DIAL) and the Doppler wind lidar with the range resolution is expected to measure atmospheric CO2 profiles and wind profiles in the atmospheric boundary layer and lower troposphere from a ground platform. We have succeeded to develop a scanning 1.6 μm DIAL and incoherent Doppler lidar system for simultaneously measuring CO2 concentration and wind speed profiles. Our 1.6 μm DIAL system consists of the Optical Parametric Generator (OPG) transmitter that excited by the LD pumped Nd: YAG laser with high repetition rate (500 Hz) and the receiving optics that included the near-infrared photomultiplier tube with high quantum efficiency operating at the photon counting mode, a fiber Bragg grating (FBG) filter to detect a Doppler shift, and a 25 cm telescope [1] [2]. We had developed an optical parametric oscillator (OPO) system for 1.6 μm CO2 DIAL[3]. To achieve continuous tuning of the resonant OPO output without mode hopping, it is necessary to vary the OPO cavity length synchronously with the seed-frequency. On the other hand, the OPG does not require a cavity and instead rely on sufficient conversion efficiency to be obtained with a single pass through the crystal. The single-frequency oscillation of the OPG was achieved by injection seeding. The CO2-DIAL was operated with the range-height indicator (RHI) mode, and the 2-D measurement provided inhomogeneity in the boundary layer. Vertical CO2 concentration profiles and wind profiles were also measured simultaneously. The elevation angle was fixed at 52 deg and CO2 concentration profiles were obtained up to 1 km altitude with 200 m height resolution. Vertical

  9. Ovarian volume throughout life

    DEFF Research Database (Denmark)

    Kelsey, Thomas W; Dodwell, Sarah K; Wilkinson, A Graham

    2013-01-01

    cancer. To date there is no normative model of ovarian volume throughout life. By searching the published literature for ovarian volume in healthy females, and using our own data from multiple sources (combined n=59,994) we have generated and robustly validated the first model of ovarian volume from...... to about 2.8 mL (95% CI 2.7-2.9 mL) at the menopause and smaller volumes thereafter. Our model allows us to generate normal values and ranges for ovarian volume throughout life. This is the first validated normative model of ovarian volume from conception to old age; it will be of use in the diagnosis...

  10. Influence of the small substitution of Z=Ni, Cu, Cr, V for Fe on the magnetic, magnetocaloric, and magnetoelastic properties of LaFe{sub 11.4}Si{sub 1.6}

    Energy Technology Data Exchange (ETDEWEB)

    Pathak, Arjun K., E-mail: pathak@siu.ed [Department of Physics, Southern Illinois University Carbondale, 1245 Lincoln Dr., Carbondale, IL 62901 (United States); Basnyat, Prakash; Dubenko, Igor [Department of Physics, Southern Illinois University Carbondale, 1245 Lincoln Dr., Carbondale, IL 62901 (United States); Stadler, Shane [Department of Physics and Astronomy, Louisiana State University, Baton Rouge, LA 70803 (United States); Ali, Naushad [Department of Physics, Southern Illinois University Carbondale, 1245 Lincoln Dr., Carbondale, IL 62901 (United States)

    2010-03-15

    We have studied the magnetic, magnetocaloric, and magnetostriction properties of LaFe{sub 11.4}Si{sub 1.6} and La(Fe{sub 0.99}Z{sub 0.01}){sub 11.4}Si{sub 1.6} (Z=Ni, Cu, Cr, V) compounds using magnetization and strain gauge techniques. It was found that substitution of 1% of the Fe by Z-elements results in an increase in the Curie temperature (T{sub C}), and affects the magnetostriction and magnetocaloric properties of the parent compound, LaFe{sub 11.4}Si{sub 1.6}. A maximum shift in T{sub C} of about 11 K, and significantly smaller hysteresis losses in the vicinity of T{sub C} compared with those of the base compound, were found for Z=V. The maximum magnetovolume coupling constant was estimated to be n{sub dd}approx2.7x10{sup -3} (mu{sub B}/Fe atom){sup -2} for the parent compound. The changes in the volume magnetostriction, the magnetovolume coupling constant, and the magnetocaloric properties are strongly correlated with composition. The relative effects of the variation in cell parameters and electron concentration on the magnetostriction, T{sub C}, and the magnetocaloric properties are discussed.

  11. 1-6月龄婴儿重症肺炎合并呼吸衰竭的临床分析%Clinical analysis on 20 1- 6 months old infants who have severe pneumonia combine with respiratory failure

    Institute of Scientific and Technical Information of China (English)

    林正伦; 蔡杰荣; 程远; 孔卫乾

    2011-01-01

    目的 分析总结1~6月龄婴儿重症肺炎合并呼吸衰竭的临床表现和治疗方法.方法 回顾性分析广州市番禺区中心医院儿科2007-2010年3年间收治的1~6月龄婴儿重症肺炎合并呼吸衰竭20例临床资料.结果 1~6月龄婴儿重症肺炎合并呼吸衰竭临床表现严重,死亡率高.结论 1~6月龄婴儿重症肺炎合并呼吸衰竭是儿科临床危重急症,早期发现、早期干预、早插快拔是治疗关键.%Objective To analyze and summarize 1-6 month impact of severe pneumonia with respiratory failure in children clinical manifestations and treatment. Methods Retrospective analysis of Panyu District, Guangzhou City, Central Hospital of Pediatrics 3 years 2007-2010 1 ~ 6 shadow on children admitted with severe pneumonia with respiratory failure in 20 patients with clinical data. Results 1~6 month shadow children with severe pneumonia with respiratory failure severe clinical manifestations and high mortality. Conclusion 1-6 month impact of severe pneumonia with respiratory failure in children is a pediatric clinical critical illness, early detection, early intervention, early intubation is the treatment of rapid extubation in the key treatment strategies.

  12. The Effect of KangLaite and 5-Fluorouracil on proliferation and apoptosis of Hepa1-6 cell%康莱特联合5-Fu对肝癌细胞Heap1-6的作用

    Institute of Scientific and Technical Information of China (English)

    刘燕燕; 赵和平

    2010-01-01

    目的 研究康莱特(KLT)联用半量最佳体外抑瘤浓度5-氟尿嘧啶(5-Fu)、单药全量5-Fu对体外小鼠Heap1-6肝癌细胞增殖及凋亡的影响.方法 采用MTT法检测各药物组对细胞活性的影响;采用流式细胞术分析各药物组对肝癌细胞株Hepa1-6细胞周期的影响.,结果 MTT示:康莱特联用半量5-Fu较单药全量5-Fu的抗肿瘤效果显著;高剂量康莱特联合半量5-Fu的抗肝癌效果较低、中剂量组联合组显著.流式细胞术示:不同浓度的康莱特联合半量5-Fu较全量5-Fu的促凋亡作用差异在中剂量时差异无显著性.结论 中剂量的康莱特联用半量5-Fu对肿瘤细胞的抑制作用及促凋亡作用与单药5-Fu的疗效相当,可以指导临床用药.

  13. Mean nuclear volume

    DEFF Research Database (Denmark)

    Mogensen, O.; Sørensen, Flemming Brandt; Bichel, P.

    1999-01-01

    We evaluated the following nine parameters with respect to their prognostic value in females with endometrial cancer: four stereologic parameters [mean nuclear volume (MNV), nuclear volume fraction, nuclear index and mitotic index], the immunohistochemical expression of cancer antigen (CA125...

  14. Highly Efficient and Versatile Synthesis of Some Important Precursors from 1,6-Anhydrous-β-D-glucopyranose as a Green Starting Material

    Institute of Scientific and Technical Information of China (English)

    WEI Guohua; CAI Chao; DU Yuguo

    2009-01-01

    Some important precursors (1,6-anhydrous-2-deoxy-2-azido-β-D-glucopyranose (3),1,6-anhydrous-2-deoxy-2-azido-3,4-di-O-benzyl-β-D-mannopyranose (5), 1,6:2,3-dianhydrouso-β-D-glucopyranose (6), 1,6-anhydrous-3-deoxy-3-azido-β-D-glucopyranose (10) and 1,6-anhydrous-2,4-di-O-benzoyl-β-D-glucopyranose (11)) for complex oligosaccharide synthesis were readily prepared from a green starting material 1,6-anhydrous-β-D-glucopyranose in one or two steps with moderate to high yields.These improved methods established herein will greatly facilitate the assembly of some complex oligosaccharides for the biological study.

  15. The crystal structure of a new polymorph of hexaaquanickel(II bis(6-oxo-1,6-dihydropyridine-3-carboxylate

    Directory of Open Access Journals (Sweden)

    Rubén Pérez-Aguirre

    2015-12-01

    Full Text Available In a new polymorph of the title salt, [Ni(H2O6](C6H4NO32, the metal atom of the cationic complex lies on a symmetry centre and is coordinated by six water molecules to provide a quite regular octahedral coordination environment. These cations interact with 6-oxo-1,6-dihydropyridine-3-carboxylate anions through electrostatic interactions and by means of O—H...O and N—H...O hydrogen bonds involving the carboxylate, keto and protonated imine groups of the anion, and the coordinating water molecules from the cationic complex entity to generate a supramolecular three-dimensional architecture. The previously reported polymorph of this compound presents a network of hydrogen bonds, in which the organic anions establish mutual hydrogen-bonding interactions involving their keto and protonated imine groups.

  16. First principle study of the interaction of elemental Hg with small neutral, anionic and cationic Pd ( = 1-6) clusters

    Indian Academy of Sciences (India)

    Shamoon Ahmad Siddiqui; Nadir Bouarissa

    2013-11-01

    Density functional theory (DFT)-based calculations have been performed so as to study the interaction of elemental mercury (Hg) with small neutral, cationic and anionic palladium clusters (Pd, = 1-6). Results of these calculations clearly indicate that frontier molecular orbital (FMO) theory is a useful method to predict the selectivity of Hg adsorption. Binding energies of Hg on cationic Pd clusters are generally found to be greater than those on neutral and anionic clusters. Results of natural bond orbital (NBO) analysis show that the flow of electrons in the neutral and charged complexes is mainly due to s orbitals of Hg. NBO analysis also indicates that, in most of the cases, the binding energies of Hg with Pdn clusters are directly proportional to charge transfer, i.e., greater the charge transfer, higher is the binding energy.

  17. Branching beta 1-6N-acetylglucosaminetransferases and polylactosamine expression in mouse F9 teratocarcinoma cells and differentiated counterparts.

    Science.gov (United States)

    Heffernan, M; Lotan, R; Amos, B; Palcic, M; Takano, R; Dennis, J W

    1993-01-15

    beta-All-trans-retinoic acid (RA)-induced endodermal differentiation of mouse F9 teratocarcinoma cells is accompanied by changes in glycoprotein glycosylation, including expression of i antigen (i.e. polylactosamine) and leukophytohemagglutinin-reactive oligosaccharides (i.e. -GlcNAc beta 1-6Man alpha 1-6-branched N-linked). We have used the F9 teratocarcinoma cells as a model to study developmental regulation of glycosyltransferase activities which are responsible for the biosynthesis of beta 1-6GlcNAc-branched N- and O-linked oligosaccharides and polylactosamine. Growth of F9 cells in the presence of 10(-6) M RA for 4 days increased core 2 GlcNAc transferase and GlcNAc transferase V activities by 13- and 6-fold, respectively, whereas the activities of GlcNAc transferase I, beta 1-3GlcNAc transferase (i), beta 1-4Gal transferase, and beta 1-3Gal transferase increased 2-4-fold. Induction of glycosyltransferase activities by RA was dose-dependent and showed a biphasic response with approximately half of the increase observed 3 days after RA treatment and the remainder occurred by day 4. PYS-2, a parietal endoderm cell line, showed levels of glycosyltransferase activities similar to those of RA-treated F9 cells. Glycosyltransferase activities in the RA-resistant F9 cell line (RA-3-10) were low and showed only a small induction by RA. These observations suggest that differentiation of F9 cells is closely associated with induction of multiple glycosyltransferase activities, with most pronounced increases in GlcNAc transferase V and 2',5'-tetradenylate (core 2) GlcNAc transferase. The increase in GlcNAc transferase V was also reflected by the 4-6-fold increase in the binding of 125I-leukophytohemagglutinin to several cellular glycoproteins, which occurred after 3 days of RA treatment. The endo-beta-galactosidase-sensitive polylactosamine content of membrane glycoproteins and, in particular, the LAMP-1 glycoprotein was markedly increased after RA treatment of F9 cells

  18. Double cascade erbium fiber laser at 1.7 µm, 2.7 µm, and 1.6 µm

    OpenAIRE

    Schneider, J; Frerichs, Ch.; Carbonnier, C.; Unrau, U.B.; Pollnau, M.; LÜthy, W.; Weber, H.P.

    1996-01-01

    The output power of the erbium laser at 2.7 um (4I11/2 -> 4I13/2) is enhanced due to simultaneous laser action at 1.7 um (4S3/2 -> 4I9/2) and 1.6 um (4I13/2 -> 4I15/2) in an Er3+-doped fluorozirconate fiber. The laser cascade overwhelms the saturation effect for the transition at 2.7 um by suppressing the laser transition at 850 nm (4S3/2 -> 4I13/2) with lasing at 1.7 um [1]. The population of the level 4S3/2 occurs for pump wavelengths around 800 nm due to strong pump excited state absorptio...

  19. Identification of substituted 2-thio-6-oxo-1,6-dihydropyrimidines as inhibitors of human lactate dehydrogenase.

    Science.gov (United States)

    Dragovich, Peter S; Fauber, Benjamin P; Corson, Laura B; Ding, Charles Z; Eigenbrot, Charles; Ge, HongXiu; Giannetti, Anthony M; Hunsaker, Thomas; Labadie, Sharada; Liu, Yichin; Malek, Shiva; Pan, Borlan; Peterson, David; Pitts, Keith; Purkey, Hans E; Sideris, Steve; Ultsch, Mark; VanderPorten, Erica; Wei, BinQing; Xu, Qing; Yen, Ivana; Yue, Qin; Zhang, Huihui; Zhang, Xuying

    2013-06-01

    A novel 2-thio-6-oxo-1,6-dihydropyrimidine-containing inhibitor of human lactate dehydrogenase (LDH) was identified by high-throughput screening (IC50=8.1 μM). Biochemical, surface plasmon resonance, and saturation transfer difference NMR experiments indicated that the compound specifically associated with human LDHA in a manner that required simultaneous binding of the NADH co-factor. Structural variation of the screening hit resulted in significant improvements in LDHA biochemical inhibition activity (best IC50=0.48 μM). A crystal structure of an optimized compound bound to human LDHA was obtained and explained many of the observed structure-activity relationships. Copyright © 2013 Elsevier Ltd. All rights reserved.

  20. Asymmetric Au-catalyzed cycloisomerization of 1,6-enynes: An entry to bicyclo[4.1.0]heptene

    Directory of Open Access Journals (Sweden)

    Alexandre Pradal

    2011-07-01

    Full Text Available A comprehensive study on the asymmetric gold-catalyzed cycloisomerization reaction of heteroatom tethered 1,6-enynes is described. The cycloisomerization reactions were conducted in the presence of the chiral cationic Au(I catalyst consisting of (R-4-MeO-3,5-(t-Bu2-MeOBIPHEP-(AuCl2 complex and silver salts (AgOTf or AgNTf2 in toluene under mild conditions to afford functionalized bicyclo[4.1.0]heptene derivatives. The reaction conditions were found to be highly substrate-dependent, the best results being obtained in the case of oxygen-tethered enynes. The formation of bicyclic derivatives, including cyclopropyl pentasubstituted ones, was reported in moderate to good yields and in enantiomeric excesses up to 99%.

  1. The Structure of the Solid Electrolyte Li1.6Ag0.4So4 at 565 degree C

    DEFF Research Database (Denmark)

    Nilsson, L.; Andersen, N. H.; Kjems, Jørgen

    1982-01-01

    The structure of the high-temperature solid solution Li1.6Ag0.4SO4 has been determined from neutron and X-ray powder diffraction data. The sulphate groups form a fcc lattice where the lithium ions are found to occupy the ±(1/4, 1/4, 1/4) tetrahedral sites. The silver ions partly occupy the same...... sites and partly the larger (½, ½, ½) octahedral site. The results strongly support earlier conclusions for the fcc phase of Li2SO4. Exceptionally high temperature factors are found. The consistency of the structural model with other characteristic properties of the high-temperature fcc phase is briefly...

  2. Magnetic refrigeration down to 1.6 K for the future circular collider e^{+}e^{-}

    Directory of Open Access Journals (Sweden)

    Jakub Tkaczuk

    2017-04-01

    Full Text Available High-field superconducting rf cavities of the future circular collider e^{+}e^{-} may require a kW-range superfluid helium refrigeration down to 1.6 K. Magnetic refrigeration operating below 4.2 K can be an alternative to the compression/expansion helium refrigeration. A significant difference between this application and previous magnetic refrigerator studies is its large cooling power, up to 10^{3} times larger than the other designs. Principles of magnetic refrigeration are described and various technical solutions are compared. A numerical model for the static magnetic refrigerator is presented, validated, and adapted to the needs of the positron-electron version of the future circular collider. A preliminary design of magnetic refrigerator suitable for low temperature, kW-range cooling is studied.

  3. [Influence of poly-β-1-6-N-acetylglucosamine on biofilm formation and drug resistance of Acinetobacter baumannii].

    Science.gov (United States)

    Guo, Haina; Xiang, Jun

    2015-02-01

    Acinetobacter baumannii has emerged as one of the leading bacteria for nosocomial infections, especially in burn wards and ICUs. The bacteria can easily form biofilm and readily attach to abiotic and biotic surfaces, resulting in persistent biofilm-mediated infections. Being surrounded by self-produced extracellular polymeric substance (EPS), the microorganisms in biofilm can acquire protective property against detrimental environment and their tolerance toward antibiotics is increased. Poly-β-1-6-N-acetylglucosamine (PNAG), the common constituent of EPS in Acinetobacter baumannii, acts as the key virulence factor and plays a crucial role in biofilm formation process. This review describes the properties and functions of the PNAG and its influence on biofilm formation and drug resistance of Acinetobacter baumannii.

  4. Earthquake simulator tests and associated study of an 1/6-scale nine-story RC model

    Institute of Scientific and Technical Information of China (English)

    Sun Jingjiang; Wang Tao; Qi Hu

    2007-01-01

    Earthquake simulator tests of a 1/6-scale nine-story reinforced concrete frame-wall model are described in the paper. The test results and associated numerical simulation are summarized and discussed. Based on the test data,a relationship between maximum inter-story drift and damage state is established. Equations of variation of structural characteristics (natural frequency and equivalent stiffness) with overall drifts are derived by data fitting, which can be used to estimate structural damage state if structural characteristics can be measured. A comparison of the analytical and experimental results show that both the commonly used equivalent beam and fiber element models can simulate the nonlinear seismic response of structures very well. Finally, conclusions associated with seismic design and damage evaluation of RC structures are presented.

  5. Effects of β-(1,3-1,6)-D-glucan on irritable bowel syndrome-related colonic hypersensitivity.

    Science.gov (United States)

    Asano, Teita; Tanaka, Ken-ichiro; Suemasu, Shintaro; Ishihara, Tomoaki; Tahara, Kayoko; Suzuki, Toshio; Suzuki, Hidekazu; Fukudo, Shin; Mizushima, Tohru

    2012-04-06

    Irritable bowel syndrome (IBS) is a gastrointestinal disorder characterized by chronic abdominal pain associated with altered bowel habits. Since the prevalence of IBS is very high and thus, involves elevated health-care costs, treatment of this condition by methods other than prescribed medicines could be beneficial. β-(1,3)-D-glucan with β-(1,6) branches (β-glucan) has been used as a nutritional supplement for many years. In this study, we examined the effect of β-glucan on fecal pellet output and visceral pain response in animal models of IBS. Oral administration of β-glucan suppressed the restraint stress- or drug-induced fecal pellet output. β-Glucan also suppressed the visceral pain response to colorectal distension. These results suggest that β-glucan could be beneficial for the treatment and prevention of IBS.

  6. Ultra-Narrow Negative Flare Front Observed in Helium-10830 Å Using the1.6m New Solar Telescope

    Science.gov (United States)

    Xu, Yan; Cao, Wenda; Ding, Mingde; Kleint, Lucia; Su, Jiangtao; Liu, Chang; Ji, Haisheng; Chae, Jongchul; Jing, Ju; Cho, Kyuhyoun; Cho, Kyung-Suk; Gary, Dale E.; Wang, Haimin

    2016-05-01

    Solar flares are sudden flashes of brightness on the Sun and are often associated with coronal mass ejections and solar energetic particles that have adverse effects on the near-Earth environment. By definition, flares are usually referred to as bright features resulting from excess emission. Using the newly commissioned 1.6-m New Solar Telescope at Big Bear Solar Observatory, we show a striking “negative” flare with a narrow but unambiguous “dark” moving front observed in He I 10830 Å, which is as narrow as 340 km and is associated with distinct spectral characteristics in Hα and Mg II lines. Theoretically, such negative contrast in He I 10830 Å can be produced under special circumstances by nonthermal electron collisions or photoionization followed by recombination. Our discovery, made possible due to unprecedented spatial resolution, confirms the presence of the required plasma conditions and provides unique information in understanding the energy release and radiative transfer in solar flares.

  7. Ultra-narrow Negative Flare Front Observed in Helium-10830~\\AA\\ using the 1.6 m New Solar Telescope

    CERN Document Server

    Xu, Yan; Ding, Mingde; Kleint, Lucia; Su, Jiangtao; Liu, Chang; Ji, Haisheng; Chae, Jongchul; Jing, Ju; Cho, Kyuhyoun; Cho, Kyungsuk; Gary, Dale; Wang, Haimin

    2016-01-01

    Solar flares are sudden flashes of brightness on the Sun and are often associated with coronal mass ejections and solar energetic particles which have adverse effects in the near Earth environment. By definition, flares are usually referred to bright features resulting from excess emission. Using the newly commissioned 1.6~m New Solar Telescope at Big Bear Solar Observatory, here we show a striking "negative" flare with a narrow, but unambiguous "dark" moving front observed in He I 10830 \\AA, which is as narrow as 340 km and is associated with distinct spectral characteristics in H-alpha and Mg II lines. Theoretically, such negative contrast in He I 10830 \\AA\\ can be produced under special circumstances, by nonthermal-electron collisions, or photoionization followed by recombination. Our discovery, made possible due to unprecedented spatial resolution, confirms the presence of the required plasma conditions and provides unique information in understanding the energy release and radiative transfer in astronomi...

  8. The organization of divalent cations in the active site of cadmium Escherichia coli fructose-1,6-bisphosphate aldolase.

    Science.gov (United States)

    Hall, David R; Kemp, Lauris E; Leonard, Gordon A; Marshall, Karen; Berry, Alan; Hunter, William N

    2003-03-01

    Previously determined crystal structures of the zinc enzyme Escherichia coli class II fructose-1,6-bisphosphate aldolase display good agreement for the protein structure but a differing metal-ion organization in the active site. The structure of the enzyme with Cd(2+) in place of Zn(2+) has now been determined to 2.0 A resolution to facilitate cation identification. The protein structure was essentially identical to other structures and five Cd(2+) positions were identified. Two of the cations are at the active site; one corresponds to the catalytic ion and the other provides a structural contribution. These Cd(2+) sites are equivalent to two Zn(2+) ions observed when the enzyme is complexed with a transition-state mimic and confirm our assignment of the roles played by these ions.

  9. NASICON-related Na3.4Mn0.4Fe1.6(PO43

    Directory of Open Access Journals (Sweden)

    Nikolay S. Slobodyanik

    2012-07-01

    Full Text Available The solid solution, sodium [iron(III/manganese(II] tris(orthophosphate, Na3.4Mn0.4Fe1.6(PO43, was obtained using a flux method. Its crystal structure is related to that of NASICON-type compounds. The [(Mn/Fe2(PO43] framework is built up from an (Mn/FeO6 octahedron (site symmetry 3., with a mixed Mn/Fe occupancy, and a PO4 tetrahedron (site symmetry .2. The Na+ cations are distributed over two partially occupied sites in the cavities of the framework. One Na+ cation (site symmetry -3. is surrounded by six O atoms, whereas the other Na+ cation (site symmetry .2 is surrounded by eight O atoms.

  10. Study of the fructose 6-phosphate/fructose 1,6-bi-phosphate cycle in the liver in vivo.

    Science.gov (United States)

    Van Schaftingen, E; Hue, L; Hers, H G

    1980-10-15

    1. The method proposed by Rognstad & Katz [(1976) Arch, Biochem, Biophys, 177, 337-345] for the determination of the fructose 6-phosphate/fructose 1,6-bisphosphate cycle by the randomization of carbon between C-1 and C-6 of glucose glucose formed from [1-14C] galactose was applied to anaesthetized rats and conscious mice. 2. It was checked that the hydrolysis of fructose 6-phosphate by glucose 6-phosphatase is too weak to invalidate the method. The participation of the Cori cycle in the randomization was negligible within the short experimental period used (2-4 min). 3. No detectable randomization of carbon was observed in starved animals, indicating that phosphofructokinase is inactive in this experimental condition. 4. Randomization of carbon was detected as soon as 1 min after administration of [1-14C] galactose to fed animals and was maximal at about 3-4 min. It was calculated that on average 15% of the glucose formed by the liver to fed rats was recycled through the triose phosphates. The extent of cycling was quite variable. Recycling was also observed in starved rats in which glucose had been administered intravenously 10 min previously. In these animals, recycling was completely inhibited by glucagon. 5. The main factors that appear to be responsible for the very large changes in recycling observed in various experimental conditions are the concentrations of fructose 1,6-bisphosphate and of fructose 6-phosphate and also the affinity of phosphofructokinase for fructose 6-phosphate. The concentration of nucleotides does not seem to play a role.

  11. Identifying a kinase network regulating FGF14:Nav1.6 complex assembly using split-luciferase complementation.

    Directory of Open Access Journals (Sweden)

    Wei-Chun Hsu

    Full Text Available Kinases play fundamental roles in the brain. Through complex signaling pathways, kinases regulate the strength of protein:protein interactions (PPI influencing cell cycle, signal transduction, and electrical activity of neurons. Changes induced by kinases on neuronal excitability, synaptic plasticity and brain connectivity are linked to complex brain disorders, but the molecular mechanisms underlying these cellular events remain for the most part elusive. To further our understanding of brain disease, new methods for rapidly surveying kinase pathways in the cellular context are needed. The bioluminescence-based luciferase complementation assay (LCA is a powerful, versatile toolkit for the exploration of PPI. LCA relies on the complementation of two firefly luciferase protein fragments that are functionally reconstituted into the full luciferase enzyme by two interacting binding partners. Here, we applied LCA in live cells to assay 12 kinase pathways as regulators of the PPI complex formed by the voltage-gated sodium channel, Nav1.6, a transmembrane ion channel that elicits the action potential in neurons and mediates synaptic transmission, and its multivalent accessory protein, the fibroblast growth factor 14 (FGF14. Through extensive dose-dependent validations of structurally-diverse kinase inhibitors and hierarchical clustering, we identified the PI3K/Akt pathway, the cell-cycle regulator Wee1 kinase, and protein kinase C (PKC as prospective regulatory nodes of neuronal excitability through modulation of the FGF14:Nav1.6 complex. Ingenuity Pathway Analysis shows convergence of these pathways on glycogen synthase kinase 3 (GSK3 and functional assays demonstrate that inhibition of GSK3 impairs excitability of hippocampal neurons. This combined approach provides a versatile toolkit for rapidly surveying PPI signaling, allowing the discovery of new modular pathways centered on GSK3 that might be the basis for functional alterations between the

  12. Active Site Loop Dynamics of a Class IIa Fructose 1,6-Bisphosphate Aldolase from Mycobacterium tuberculosis

    Energy Technology Data Exchange (ETDEWEB)

    Pegan, Scott D. [Univ. of Denver, CO (United States); Rukseree, Kamolchanok [National Center for Genetic Engineering and Biotechnology (BIOTEC), Tha Khlong (Thailand); Capodagli, Glenn C. [Univ. of Denver, CO (United States); Baker, Erica A. [Univ. of Denver, CO (United States); Krasnykh, Olga [Univ. of Illinois, Chicago, IL (United States); Franzblau, Scott G. [Univ. of Illinois, Chicago, IL (United States); Mesecar, Andrew D. [Purdue Univ., West Lafayette, IN (United States)

    2013-01-08

    The class II fructose 1,6-bisphosphate aldolases (FBAs, EC 4.1.2.13) comprises one of two families of aldolases. Instead of forming a Schiff base intermediate using an ε-amino group of a lysine side chain, class II FBAs utilize Zn(II) to stabilize a proposed hydroxyenolate intermediate (HEI) in the reversible cleavage of fructose 1,6-bisphosphate, forming glyceraldehyde 3-phosphate and dihydroxyacetone phosphate (DHAP). As class II FBAs have been shown to be essential in pathogenic bacteria, focus has been placed on these enzymes as potential antibacterial targets. Although structural studies of class II FBAs from Mycobacterium tuberculosis (MtFBA), other bacteria, and protozoa have been reported, the structure of the active site loop responsible for catalyzing the protonation–deprotonation steps of the reaction for class II FBAs has not yet been observed. We therefore utilized the potent class II FBA inhibitor phosphoglycolohydroxamate (PGH) as a mimic of the HEI- and DHAP-bound form of the enzyme and determined the X-ray structure of the MtFBA–PGH complex to 1.58 Å. Remarkably, we are able to observe well-defined electron density for the previously elusive active site loop of MtFBA trapped in a catalytically competent orientation. Utilization of this structural information and site-directed mutagenesis and kinetic studies conducted on a series of residues within the active site loop revealed that E169 facilitates a water-mediated deprotonation–protonation step of the MtFBA reaction mechanism. Furthermore, solvent isotope effects on MtFBA and catalytically relevant mutants were used to probe the effect of loop flexibility on catalytic efficiency. Additionally, we also reveal the structure of MtFBA in its holoenzyme form.

  13. Rational Design Synthesis and Evaluation of First Generation Inhibitors of the Giardia Lamblia Fructose-1 6-biphosphate Aldolase

    Energy Technology Data Exchange (ETDEWEB)

    Z Li; Z Liu; D Cho; J Zou; M Gong; R Breece; A Galkin; L Li; H Zhao; et al.

    2011-12-31

    Inhibitors of the Giardia lamblia fructose 1,6-bisphosphate aldolase (GlFBPA), which transforms fructose 1,6-bisphosphate (FBP) to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate, were designed based on 3-hydroxy-2-pyridone and 1,2-dihydroxypyridine scaffolds that position two negatively charged tetrahedral groups for interaction with substrate phosphate binding residues, a hydrogen bond donor to the catalytic Asp83, and a Zn{sup 2+} binding group. The inhibition activities for the GlFBPA catalyzed reaction of FBP of the prepared alkyl phosphonate/phosphate substituted 3-hydroxy-2-pyridinones and a dihydroxypyridine were determined. The 3-hydroxy-2-pyridone inhibitor 8 was found to bind to GlFBPA with an affinity (K{sub i} = 14 {micro}M) that is comparable to that of FBP (K{sub m} = 2 {micro}M) or its inert analog TBP (K{sub i} = 1 {micro}M). The X-ray structure of the GlFBPA-inhibitor 8 complex (2.3 {angstrom}) shows that 8 binds to the active site in the manner predicted by in silico docking with the exception of coordination with Zn{sup 2+}. The observed distances and orientation of the pyridone ring O=C-C-OH relative to Zn{sup 2+} are not consistent with a strong interaction. To determine if Zn{sup 2+} coordination occurs in the GlFBPA-inhibitor 8 complex in solution, EXAFS spectra were measured. A four coordinate geometry comprised of the three enzyme histidine ligands and an oxygen atom from the pyridone ring O=C-C-OH was indicated. Analysis of the Zn{sup 2+} coordination geometries in recently reported structures of class II FBPAs suggests that strong Zn{sup 2+} coordination is reserved for the enediolate-like transition state, accounting for minimal contribution of Zn{sup 2+} coordination to binding of 8 to GlFBPA.

  14. Structural and functional characterization of methicillin-resistant Staphylococcus aureus's class IIb fructose 1,6-bisphosphate aldolase.

    Science.gov (United States)

    Capodagli, Glenn C; Lee, Stephen A; Boehm, Kyle J; Brady, Kristin M; Pegan, Scott D

    2014-12-09

    Staphylococcus aureus is one of the most common nosocomial sources of soft-tissue and skin infections and has more recently become prevalent in the community setting as well. Since the use of penicillins to combat S. aureus infections in the 1940s, the bacterium has been notorious for developing resistances to antibiotics, such as methicillin-resistant Staphylococcus aureus (MRSA). With the persistence of MRSA as well as many other drug resistant bacteria and parasites, there is a growing need to focus on new pharmacological targets. Recently, class II fructose 1,6-bisphosphate aldolases (FBAs) have garnered attention to fill this role. Regrettably, scarce biochemical data and no structural data are currently available for the class II FBA found in MRSA (SaFBA). With the recent finding of a flexible active site zinc-binding loop (Z-Loop) in class IIa FBAs and its potential for broad spectrum class II FBA inhibition, the lack of information regarding this feature of class IIb FBAs, such as SaFBA, has been limiting for further Z-loop inhibitor development. Therefore, we elucidated the crystal structure of SaFBA to 2.1 Å allowing for a more direct structural analysis of SaFBA. Furthermore, we determined the KM for one of SaFBA's substrates, fructose 1,6-bisphosphate, as well as performed mode of inhibition studies for an inhibitor that takes advantage of the Z-loop's flexibility. Together the data offers insight into a class IIb FBA from a pervasively drug resistant bacterium and a comparison of Z-loops and other features between the different subtypes of class II FBAs.

  15. New insight into the binding modes of TNP-AMP to human liver fructose-1,6-bisphosphatase

    Science.gov (United States)

    Han, Xinya; Huang, Yunyuan; Zhang, Rui; Xiao, San; Zhu, Shuaihuan; Qin, Nian; Hong, Zongqin; Wei, Lin; Feng, Jiangtao; Ren, Yanliang; Feng, Lingling; Wan, Jian

    2016-08-01

    Human liver fructose-1,6-bisphosphatase (FBPase) contains two binding sites, a substrate fructose-1,6-bisphosphate (FBP) active site and an adenosine monophosphate (AMP) allosteric site. The FBP active site works by stabilizing the FBPase, and the allosteric site impairs the activity of FBPase through its binding of a nonsubstrate molecule. The fluorescent AMP analogue, 2‧,3‧-O-(2,4,6-trinitrophenyl)adenosine 5‧-monophosphate (TNP-AMP) has been used as a fluorescent probe as it is able to competitively inhibit AMP binding to the AMP allosteric site and, therefore, could be used for exploring the binding modes of inhibitors targeted on the allosteric site. In this study, we have re-examined the binding modes of TNP-AMP to FBPase. However, our present enzyme kinetic assays show that AMP and FBP both can reduce the fluorescence from the bound TNP-AMP through competition for FBPase, suggesting that TNP-AMP binds not only to the AMP allosteric site but also to the FBP active site. Mutagenesis assays of K274L (located in the FBP active site) show that the residue K274 is very important for TNP-AMP to bind to the active site of FBPase. The results further prove that TNP-AMP is able to bind individually to the both sites. Our present study provides a new insight into the binding mechanism of TNP-AMP to the FBPase. The TNP-AMP fluorescent probe can be used to exam the binding site of an inhibitor (the active site or the allosteric site) using FBPase saturated by AMP and FBP, respectively, or the K247L mutant FBPase.

  16. Two-Year Chronic Bioassay study of Rats Exposed to a 1.6 GHz Radiofrequency Signal.

    Energy Technology Data Exchange (ETDEWEB)

    Anderson, Larry E.; Sheen, David M.; Wilson, Bary W.; Grumbein, Sondra L.; Creim, Jeffrey A.; Sasser, Lyle B.

    2004-08-06

    The purpose of this study was to determine whether long-term exposure to a 1.6 GHz radiofrequency (RF) field would affect the incidence of cancer in Fischer 344 rats. Thirty-six timed-pregnant rats were randomly assigned to each of three treatment groups: two groups exposed to a far-field RF Iridium signal and a third group that was sham exposed. Exposures were chosen such that the brain SAR in the fetuses was 0.16 W/kg. Whole-body far-field exposures were initiated at 19 days of gestation and continued at 2 h/day, 7 days/week for dams and pups after parturition until weaning ({approx}23 days old). The offspring (700) of these dams were selected, 90 males and 90 females for each near-field treatment group, with SAR levels in the brain calculated to be as follows: (1) 1.6 W/kg, (2) 0.16 W/kg and (3) near-field sham controls with an additional 80 males and 80 females as shelf controls. Confining, head-first, near-field exposures of 2 h/day, 5 days/week were initiated when the offspring were 36 + 1 days old and continued until the rats were 2 years old. No statistically significant differences were observed among treatment groups for number of live pups/litter, survival index and weaning weights, nor were there differences in clinical signs or neoplastic lesions among the treatment groups. The percentages of animals surviving at the end of the near-field exposure were not different among the male groups. In females a significant decrease in survival time was observed for the case control group.

  17. Water Vapor Self-Continuum by Cavity Ring Down Spectroscopy in the 1.6 Micron Transparency Window

    Science.gov (United States)

    Campargue, Alain; Kassi, Samir; Mondelain, Didier

    2014-06-01

    Since its discovery one century ago, a deep and unresolved controversy remains on the nature of the water vapor continuum. Several interpretations are proposed: accumulated effect of the distant wings of many individual spectral lines, metastable or true bound water dimers, collision-induced absorption. The atmospheric science community has largely sidestepped this controversy, and has adopted a pragmatic approach: most radiative transfer codes used in climate modelling, numerical weather prediction and remote sensing use the MT_CKD model which is a semi-empirical formulation of the continuum The MT_CKD cross-sections were tuned to available observations in the mid-infrared but in the absence of experimental constraints, the extrapolated near infrared (NIR) values are much more hazardous. Due to the weakness of the broadband absorption signal to be measured, very few measurements of the water vapor continuum are available in the NIR windows especially for temperature conditions relevant for our atmosphere. This is in particular the case for the 1.6 μm window where the very few available measurements show a large disagreement. Here we present the first measurements of the water vapor self-continuum cross-sections in the 1.6 μm window by cavity ring down spectroscopy (CRDS). The pressure dependence of the absorption continuum was investigated during pressure cycles up to 12 Torr for selected wavenumber values. The continuum level is observed to deviate from the expected quadratic dependence with pressure. This deviation is interpreted as due to a significant contribution of water adsorbed on the super mirrors to the cavity loss rate. The pressure dependence is well reproduced by a second order polynomial. We interpret the linear and quadratic terms as the adsorbed water and vapour water contribution, respectively. The derived self-continuum cross sections, measured between 5875 and 6450 wn, shows a minimum value around 6300 wn. These cross sections will be compared

  18. One-pot synthesis of dibenzo[b,h][1,6]naphthyridines from 2-acetylaminobenzaldehyde: application to a fluorescent DNA-binding compound.

    Science.gov (United States)

    Okuma, Kentaro; Koga, Tomohiro; Ozaki, Saori; Suzuki, Yutaro; Horigami, Kenta; Nagahora, Noriyoshi; Shioji, Kosei; Fukuda, Masatora; Deshimaru, Masanobu

    2014-12-21

    Dibenzo[b,h][1,6]naphthyridines were synthesized in one pot by reacting 2-acetylaminobenzaldehyde with methyl ketones under basic conditions via four sequential condensation reactions. This method was also applied to the synthesis of 1,2-dihydroquinolines. 6-Methyl-1,6-dibenzonaphthyridinium triflates showed strong fluorescence, and the fluorescence intensities were changed upon intercalation into double-stranded DNA.

  19. Organocatalytic Asymmetric 1,6-Addition/1,4-Addition Sequence to 2,4-Dienals for the Synthesis of Chiral Chromans

    DEFF Research Database (Denmark)

    Poulsen, Pernille H; Feu, Karla Santos; Paz, Bruno Matos;

    2015-01-01

    A novel asymmetric organocatalytic 1,6-addition/1,4-addition sequence to 2,4-dienals is described. Based on a 1,6-Friedel-Crafts/1,4-oxa-Michael cascade, the organocatalyst directs the reaction of hydroxyarenes with a vinylogous iminium-ion intermediate to give only one out of four possible regio...

  20. Draft Genome Sequence of the Plant Growth–Promoting Pseudomonas punonensis Strain D1-6 Isolated from the Desert Plant Erodium hirtum in Jordan

    Science.gov (United States)

    Lafi, Feras F.; AlBladi, Maha L.; Salem, Nida M.; Al-Banna, Luma; Alam, Intikhab; Bajic, Vladimir B.

    2017-01-01

    ABSTRACT Pseudomonas punonensis strain D1-6 was isolated from roots of the desert plant Erodium hirtum, near the Dead Sea in Jordan. The genome of strain D1-6 reveals several key plant growth–promoting and herbicide-resistance genes, indicating a possible specialized role for this endophyte. PMID:28082490

  1. Identification and expression of fructose-1,6-bisphosphate aldolase genes and their relations to oil content in developing seeds of tea oil tree (Camellia oleifera)

    Science.gov (United States)

    Tea oil tree (Camellia oleifera, Co) provides a fine edible oil source in China. Tea oil from the seeds is very beneficial to human health. Fructose-1,6-bisphosphate aldolase (FBA) hydrolyzes fructose-1,6-bisphosphate into dihydroxyacetone phosphate and glyceraldehyde 3-phosphate, two critical metab...

  2. Draft Genome Sequence of the Plant Growth–Promoting Pseudomonas punonensis Strain D1-6 Isolated from the Desert Plant Erodium hirtum in Jordan

    KAUST Repository

    Lafi, Feras Fawzi

    2017-01-13

    Pseudomonas punonensis strain D1-6 was isolated from roots of the desert plant Erodium hirtum, near the Dead Sea in Jordan. The genome of strain D1-6 reveals several key plant growth-promoting and herbicide-resistance genes, indicating a possible specialized role for this endophyte.

  3. N-acetylcysteine and fructose-1,6-bisphosphate: immunomodulatory effects on mononuclear cell culture N-acetilcisteína e frutose-1,6-bisfosfato: efeito imunomodulador em cultura de células mononucleares

    Directory of Open Access Journals (Sweden)

    Ricardo Obalski de Mello

    2012-04-01

    Full Text Available INTRODUCTION: Sepsis is a complex syndrome caused by an uncontrolled systemic inflammatory response. Inflammatory cytokines play a pivotal role in septic shock pathogenesis. Therapeutic strategies have been tested in order to modulate the excessive generation or function of sepsis mediators. OBJECTIVE: The objective of the present study was to investigate the therapeutic effect of N-acetylcysteine (NAC and its association with fructose-1,6-bisphosphate (FBP on T-lymphocytes proliferation, interleukin-1β (IL-1β and monocyte chemotactic protein-1 (MCP-1 levels. MATERIAL AND METHODS: Peripheral blood mononuclear cell samples were isolated from healthy individuals. T-lymphocytes were stimulated with phytohemagglutinin for 96 hours and submitted to different concentrations of NAC or NAC associated with FBP. RESULTS: NAC (10 and 15 mM and NAC (15 mM associated with FBP reduced T-lymphocytes proliferation. IL-1β levels rose in the presence of both NAC (15 mM and NAC with FBP (1.25 mM. MCP-1 levels were reduced only by NAC (15 mM associated with FBP (1.25 mM. CONCLUSION: The results suggest that both NAC itself and NAC associated with FBP inhibit cellular proliferation, acting as potent immunomodulatory agents, which corroborates its use in the treatment of inflammatory diseases.INTRODUÇÃO: A sepse é uma síndrome complexa causada pela resposta inflamatória sistêmica descontrolada. As citocinas inflamatórias representam papel central na patogênese do choque séptico. Têm sido testadas estratégias terapêuticas a fim de modular a geração ou a função excessiva de mediadores na sepse. OBJETIVO: O objetivo deste estudo foi investigar o efeito terapêutico da N-acetilcisteína (NAC e sua associação com a frutose-1,6-bisfosfato (FBP sobre a proliferação de linfócitos T e a geração de interleucina-1β (IL-1β e proteína quimiotática de monócitos 1 (MCP-1 em cultura celular. MATERIAL E MÉTODOS: Foram isoladas células mononucleares de

  4. 1,6-二磷酸果糖对体外循环期间中性粒细胞的影响%Influence of fructose 1,6-diphosphate on polymorphonuclear neutrophils during ca rdiopulmonary bypass

    Institute of Scientific and Technical Information of China (English)

    罗勇; 李世忠; 申树先; 金士翱

    2001-01-01

    目的研究体外循环下1,6-二磷酸果糖(FDP)对中性粒细胞( PMNs)活化、释放的影响.方法 20例先心病矫正术患者随机分成使用FDP(220mg/kg)的观察组(n=10)和对照组(n=10), 动态观察中性粒细胞化学发光(PM N-CL)值、中性粒细胞内髓过氧化物酶(MPO)及弹性蛋白酶(ELA)活性.结果 (1)与阻断主动脉时对比,两组各时点PMN-CL值均明显升高( P<0.05、P<0.01或P<0.001),PMN-MPO及ELA 活性降低(P<0.05或P<0.01),化学发光最高值、MPO及ELA活性最低值出现在主动脉开放后(再灌注)45分钟.(2)与对照组相比,在主动脉阻断3 0 分钟、主动脉开放后(再灌注)45分钟及90分钟,观察组PMN-CL值降低,PMN -MP O及ELA活性升高(P<0.05或P<0.01).结论 (1)体外循环时PMNs活化并释放多种活性物质,且主要发生于主动脉开放后(再灌注 )早期.(2)FDP抑制PMNs的活化,减轻了PMNs的释放反应.

  5. Relationship between fructose-1,6-diphosphate and gastrointestinal reperfusion and systemic inflammatory response%1,6二磷酸果糖对胃肠灌注与全身炎性反应关系的探讨

    Institute of Scientific and Technical Information of China (English)

    邹捍东; 程帮昌; 周敏; 周晨亮; 侯伟

    2007-01-01

    目的 探讨1,6-二磷酸果糖(FDP)对小儿心脏手术体外循环(CPB)期间胃肠灌注与全身炎性反应的关系.方法 择期心内直视手术1~12岁患儿30例,随机分为对照组(T组)和治疗组(F组),F组静注FDP,对照组静注等容量生理盐水.于切皮前(T0)、主动脉开放1 h(R1)、2 h(R2)检测胃黏膜pH(pHi)、血浆二胺氧化酶(DAO)、血浆内毒素(LPS)、血清IL-6水平.结果 T0时2组胃pHi、血DAO、LPS、IL-6差异无统计学意义(P>0.05);2组R1、R2时胃pHi值低于T0值(P<0.05),血DAO、LPS及IL-6高于T0值(P<0.05);F组R1、R2时胃pHi值高于T组(P<0.05),血DAO、LPS及IL-6低于T组(P<0.05).结论 FDP对小儿心脏手术CPB期间胃肠道具有保护作用,并可抑制全身炎性反应.

  6. The Molecular Gas Contents Of Z=1.6 (Proto)-Cluster Galaxies And Their Last Gasp Of Star Formation

    Science.gov (United States)

    Rudnick, Gregory

    2017-06-01

    In this talk I present two related topics. First, I discuss the constraints on the quenching efficiency and the age in stellar populations that we derive from deep HST G102 and G141 grism observations in a z=1.62 proto-cluster galaxy. We use the grism data to establish unambiguous membership for passive and star-forming galaxies and to measure the strength of the 4000-Angstrom break strength. We find a strongly mass-dependent passive fraction that is in excess of the field at the highest stellar masses. However, the strength of D4000 for passive galaxies is independent of mass and we interpret this as a lack of strong trends of stellar age with mass. We discuss possible explanations for our observations that there is no stellar age trend although more massive galaxies clearly quench more efficiently than lower mass galaxies. We then discuss the molecular gas properties in a set of 4 clusters at z=1.6. We find that most galaxies have gas fractions that are elevated with respect to the field for galaxies at similar redshift, stellar mass, and star formation rate. However, there is a significant cluster-to-cluster dispersion with galaxies in one cluster having molecular gas properties indistinguishable from the field. There is also some suggestion that the most quenched clusters have the lowest gas fractions but this requires more cluster molecular gas data to confirm this tentative picture.

  7. Thermoelectric Characterization of (Na1-yMy)1.6Co2O4(M=K,Ca,Sr)

    Institute of Scientific and Technical Information of China (English)

    Ying LI; Guiying XU; Maofa JIANG

    2006-01-01

    Oxide materials NaCo2O4 and (Na1-yMy)1.6Co2O4 (M=K, 0.05≤y≤0.35; M=Ca, Sr, 0.10≤y≤0.40) were prepared by a sol-gel method. Experimental results indicated that the Seebeck coefficient and the Powerfactor of NaCo2O4 were improved by doping Ca and Sr but not by K. The Power-factor of NaCo2O4 is in its maximum, 1.68×10-4 W·m-1·K-2 and 11% bigger than that of the original one when the dopant fraction of Ca was 0.1. Doping Sr makes NaCo2O4 have the biggest Power-factor, 1.68×10-4 W·m-1·K-2 that is 50% bigger than the non-doping oxide. The experimental results indicate that suitable dopants and addition amounts could improve the thermoelectric properties of NaCo2O4 greatly.

  8. The 1.6 micron near infrared nuclei of 3C radio galaxies: Jets, thermal emission or scattered light?

    CERN Document Server

    Baldi, R D; Capetti, A; Sparks, W; Macchetto, F D; O'Dea, C P; Axon, D J; Baum, S A; Quillen, A C

    2010-01-01

    Using HST NICMOS 2 observations we have measured 1.6-micron near infrared nuclear luminosities of 100 3CR radio galaxies with z<0.3, by modeling and subtracting the extended emission from the host galaxy. We performed a multi-wavelength statistical analysis (including optical and radio data) of the properties of the nuclei following classification of the objects into FRI and FRII, and LIG (low-ionization galaxies), HIG (high-ionization galaxies) and BLO (broad-lined objects) using the radio morphology and optical spectra, respectively. The correlations among near infrared, optical, and radio nuclear luminosity support the idea that the near infrared nuclear emission of FRIs has a non-thermal origin. Despite the difference in radio morphology, the multi-wavelength properties of FRII LIG nuclei are statistically indistinguishable from those of FRIs, an indication of a common structure of the central engine. All BLOs show an unresolved near infrared nucleus and a large near infrared excess with respect to FRI...

  9. Fructose-1,6-bisphosphate aldolases in amitochondriate protists constitute a single protein subfamily with eubacterial relationships.

    Science.gov (United States)

    Sánchez, Lidya; Horner, David; Moore, Dorothy; Henze, Katrin; Embley, T; Müller, Miklós

    2002-07-24

    Sequences of putative fructose-1,6-bisphospate aldolases (FBA) in five amitochondriate unicellular eukaryotes, the diplomonads Giardia intestinalis (published earlier) and Spironucleus barkhanus, the pelobiont Mastigamoeba balamuthi,the entamoebid Entamoeba histolytica, and the parabasalid Trichomonas vaginalis all belong to Class II of FBAs and are highly similar to each other (>48% amino acid identity). The five protist sequences, however, do not form a monophyletic group. Diplomonad FBAs share a most recent common ancestor, while FBAs of the three other protist species are part of a lineage that also includes sequences from a few eubacteria (Clostridium difficile, Treponema pallidum, Chlorobium tepidum). Both clades are part of the Type B of Class II aldolases, a complex that contains at least three additional lineages (subgroups) of enzymes. Type B enzymes are distant from Type A Class II aldolases, which consists of a number of bacterial and fungal enzymes and also contains the cytosolic FBA of Euglena gracilis. Class II aldolases are not homologous to Class I enzymes, to which animal and plant enzymes belong. The results indicate that amitochondriate protists acquired their FBAs from separate and different sources, involving lateral gene transfer from eubacteria, than did all other eukaryotes studied so far and underscore the complex composition of the glycolytic machinery in unicellular eukaryotes.

  10. Galaxy environments over cosmic time: the non-evolving radial galaxy distributions around massive galaxies since z=1.6

    CERN Document Server

    Tal, Tomer; Franx, Marijn; Leja, Joel; Wake, David A; Whitaker, Katherine E

    2013-01-01

    We present a statistical study of the environments of massive galaxies in four redshift bins between z=0.04 and z=1.6, using data from the Sloan Digital Sky Survey (SDSS) and the NEWFIRM Medium Band Survey (NMBS). We measure the projected radial distribution of galaxies in cylinders around a constant number density selected sample of massive galaxies and utilize a statistical subtraction of contaminating sources. Our analysis shows that massive primary galaxies typically live in group halos and are surrounded by 2 to 3 satellites with masses more than one-tenth of the primary galaxy mass. The cumulative stellar mass in these satellites roughly equals the mass of the primary galaxy itself. We further find that the radial number density profile of galaxies around massive primaries has not evolved significantly in either slope or overall normalization in the past 9.5 Gyr. A simplistic interpretation of this result can be taken as evidence for a lack of mergers in the studied groups and as support for a static ev...

  11. Supernovae in the Subaru Deep Field: An Initial Sample, and Type Ia Rate, out to Redshift 1.6

    CERN Document Server

    Poznanski, Dovi; Yasuda, Naoki; Foley, Ryan J; Doi, Mamoru; Filippenko, Alexei V; Fukugita, Masataka; Gal-Yam, Avishay; Jannuzi, Buell T; Morokuma, Tomoki; Oda, Takeshi; Schweiker, Heidi; Sharon, Keren; Silverman, Jeffrey M; Totani, Tomonori

    2007-01-01

    Large samples of high-redshift supernovae (SNe) are potentially powerful probes of cosmic star formation, metal enrichment, and SN physics. We present initial results from a new deep SN survey, based on re-imaging in the R, i', z' bands, of the 0.25 deg2 Subaru Deep Field (SDF), with the 8.2-m Subaru telescope and Suprime-Cam. In a single new epoch consisting of two nights of observations, we have discovered 33 SNe, down to a z'-band magnitude of 26.3 (AB). We have measured the photometric redshifts of the SN host galaxies, obtained Keck spectroscopic redshifts for 17 of the host galaxies, and classified the SNe using the Bayesian photometric algorithm of Poznanski et al. (2007) that relies on template matching. After correcting for biases in the classification, 55% of our sample consists of Type Ia supernovae and 45% of core-collapse SNe. The redshift distribution of the SNe Ia reaches z ~ 1.6, with a median of z ~ 1.2. The core-collapse SNe reach z ~ 1.0, with a median of z ~ 0.5. Our SN sample is comparabl...

  12. Unprecedented Fine Structure of a Solar Flare Revealed by the 1.6~m New Solar Telescope

    CERN Document Server

    Jing, Ju; Cao, Wenda; Liu, Chang; Gary, Dale; Wang, Haimin

    2016-01-01

    Solar flares signify the sudden release of magnetic energy and are sources of so called space weather. The fine structures (below 500 km) of flares are rarely observed and are accessible to only a few instruments world-wide. Here we present observation of a solar flare using exceptionally high resolution images from the 1.6~m New Solar Telescope (NST) equipped with high order adaptive optics at Big Bear Solar Observatory (BBSO). The observation reveals the process of the flare in unprecedented detail, including the flare ribbon propagating across the sunspots, coronal rain (made of condensing plasma) streaming down along the post-flare loops, and the chromosphere's response to the impact of coronal rain, showing fine-scale brightenings at the footpoints of the falling plasma. Taking advantage of the resolving power of the NST, we measure the cross-sectional widths of flare ribbons, post-flare loops and footpoint brighenings, which generally lie in the range of 80-200 km, well below the resolution of most curr...

  13. Fructose-1,6-bisphosphatase is a novel regulator of Wnt/β-Catenin pathway in breast cancer.

    Science.gov (United States)

    Li, Kaichun; Ying, Mingzhen; Feng, Dan; Du, Jie; Chen, Shiyu; Dan, Bing; Wang, Cuihua; Wang, Yajie

    2016-12-01

    Fructose-1,6-bisphosphatase (FBP1), the rate-limiting enzyme in gluconeogenesis, is a tumor suppressor that frequently down-regulated in cancers, especially breast cancer. Here, we provide both supporting and contradicting evidences about the expression pattern and function of FBP1 in breast cancer. Data mining of Oncomine database showed that FBP1 is commonly up-regulated in tumor tissues compared with non-tumor tissues regardless of histological type. Analysis of a large-scale cohort derived from Kaplan-Meier Plotter showed that lower FBP1 expression associated with poor clinical outcome. Genetic silencing of FBP1 reduced aerobic glycolysis and the malignant potential of breast cancer cells. Gene set enrichment analysis (GSEA) of the expression profiles of breast cancer cells (n=59) revealed that cells exhibiting high expression of FBP1 had a lower activity of Wnt/β-Catenin pathway. FBP1 down-regulation enhanced the activity of Wnt/β-Catenin pathway and increased the level of its downstream targets, including c-Myc and MMP7. Collectively, our findings indicate that elevated FBP1 is a critical modulator in breast cancer progression by altering glucose metabolism and the activity of Wnt/β-Catenin pathway.

  14. A DFT study on the mechanism of palladium-catalyzed divergent reactions of 1,6-enyne carbonates

    Indian Academy of Sciences (India)

    Xing Hui Zhang; Zhi Yuan Geng; Teng Niu; Ke Tai Wang

    2015-03-01

    The reaction mechanisms of palladium-catalyzed divergent reactions of 1,6-enyne carbonates have been investigated using DFT calculations at the B3LYP/6-31G(d,p) (LanL2DZ for Pd) level. Solvent effects on these reactions have been considered by the polarizable continuum model (PCM) for the solvent (DMF). The formation of vinylidenepyridines and vinylidenepyrrolidines were generated through 5-exo-dig cyclization or 6-endo-dig cyclization. Our calculation results suggested the following: (i) The first step of the whole cycle is the rate-determining step, which causes allenic palladium intermediate through two plausible pathways. This intermediate provides the corresponding products and releases the palladium catalyst by a subsequent hydrogen transfer and elimination process. (ii) For the catalyst CH3OPdH, the reaction could occur through two possible pathways, but 5-exo-dig cyclization is favoured over 6-endo-dig cyclization. However, when the hydrogen atom is substituted with a phenyl group, the energy barriers for 5-exo-dig cyclization or 6-endo-dig cyclization become relatively high, 18.0–28.5 kcal/mol. The computational results provide good explanation for the experimental observations.

  15. Synthesis and characterization of biodegradable acrylated polyurethane based on poly(ε-caprolactone) and 1,6-hexamethylene diisocyanate.

    Science.gov (United States)

    Alishiri, M; Shojaei, A; Abdekhodaie, M J; Yeganeh, H

    2014-09-01

    A series of biodegradable acrylic terminated polyurethanes (APUs) based on poly(ε-caprolactone) diol (PCL), aliphatic 1,6-hexamethylene diisocyanate (HDI) and hydroxyethyl methyl acrylate (HEMA) was synthesized as potential materials for hard tissue biomedical applications. PCLs with low molecular weights of 1000 and 2,000 g/mol were employed to provide different amounts of end capped urethane acrylate in APUs. To control crosslink density, a mixture of two different reactive diluents including mono-functional HEMA and bi-functional ethylene glycol dimethacrylate (EGDMA) with different weight ratios was incorporated into the APUs, called here PUAs. Morphological characteristics and mechanical properties were investigated using X-ray diffraction (XRD), differential scanning calorimetry (DSC) and dynamic mechanical analysis (DMA). DMA results indicated some degree of microphase separation between hard and soft segments; however, the microphase separation is more prominent for PUAs with higher molecular weight PCL. It was also found that the degree of crosslinking dominated greatly the formation of crystalline structure. PUAs with low crosslink density exhibited crystalline microstructure. The results also indicated that the mechanical properties of PUAs were governed considerably by crystalline microstructure, and hard segment content. All PUAs demonstrated hydrophobic behavior and were able to be degraded hydrolytically. The degradation process was closely related to the microstructure and surface tension of PUAs.

  16. Situations, Problems, and Needs in Teaching English of Grades 1-6 Teachers in the Central Region of Thailand

    Directory of Open Access Journals (Sweden)

    Kanya Kongsoongnoen

    2017-02-01

    Full Text Available The purpose of this research was to examine the situations, problems, and needs in teaching English of grades 1-6 teachers in five provinces located in the five provinces in the central region of Thailand: Lop Buri, Sing Buri, Saraburi, Suphan Buri, and Ang-Thong in 2016. A questionnaire comprising both open and closed-ended questions was sent to the English teachers in the area and 170 teachers responded back. Percentage, mean and standard deviation were applied to analyze quantitative data whereas content analysis was conducted for qualitative data. The results of the teachers’ questionnaire response indicated most participants had no degree in English, but had 1-5 year experience in teaching English. When considering on the teachers’ perceptions on teaching English using Likert Scales, their satisfaction was at a moderate level in every item (teaching situations, contents, learning activities, learning materials, and evaluation. Most teachers mentioned that lacking teaching methodologies, contents, and skills in English were of concern when teaching English, therefore, they had their students learn from the programs themselves and sometimes the teachers used the text books to teach. The findings also showed that the teachers needed teaching English training especially the English teaching methodologies. Lacking qualified teachers to teach English in primary grades was critical particularly with Grades 1-2. Except where otherwise noted, content on this site is licensed under a Creative Commons Attribution 4.0 International License

  17. Crystallization and preliminary crystallographic analysis of 2-aminophenol 1,6-dioxygenase complexed with substrate and with an inhibitor.

    Science.gov (United States)

    Li, De-Feng; Zhang, Jia-Yue; Hou, Yanjie; Liu, Lei; Liu, Shuang-Jiang; Liu, Wei

    2012-11-01

    Dioxygen activation implemented by nonhaem Fe(II) enzymes containing the 2-His-1-carboxylate facial triad has been extensively studied in recent years. Extradiol dioxygenase is the archetypal member of this superfamily and catalyzes the oxygenolytic ring opening of catechol analogues. Here, the crystallization and preliminary X-ray analysis of 2-aminophenol 1,6-dioxygenase, an enzyme representing a minor subset of extradiol dioxygenases that catalyze the fission of 2-aminophenol rather than catecholic compounds, is reported. Crystals of the holoenzyme with FeII and of complexes with the substrate 2-aminophenol and the suicide inhibitor 4-nitrocatechol were grown using the cocrystallization method under the same conditions as used for the crystallization of the apoenzyme. The crystals belonged to space group C2 and diffracted to 2.3-2.7 Å resolution; the crystal that diffracted to the highest resolution had unit-cell parameters a=270.24, b=48.39, c=108.55 Å, β=109.57°. All X-ray data sets collected from diffraction-quality crystals were suitable for structure determination.

  18. Identification of Outflows and Candidate Dual Active Galactic Nuclei in SDSS Quasars at z=0.8-1.6

    CERN Document Server

    Barrows, R Scott; Kennefick, Julia; Comerford, Julia M; Kennefick, Daniel; Berrier, Joel C

    2013-01-01

    We present a sample of 131 quasars from the Sloan Digital Sky Survey at redshifts 0.81.6 with double peaks in either of the high-ionization narrow emission lines [NeV]3426 or [NeIII]3869. These sources were selected with the intention of identifying high-redshift analogs of the z<0.8 active galactic nuclei (AGN) with double-peaked [OIII]5007 lines, which might represent AGN outflows or dual AGN. Lines of high-ionization potential are believed to originate in the inner, highly photoionized portion of the narrow line region (NLR), and we exploit this assumption to investigate the possible kinematic origins of the double-peaked lines. For comparison, we measure the [NeV]3426 and [NeIII]3869 double peaks in low-redshift (z<0.8) [OIII]-selected sources. We find that [NeV]3426 and [NeIII]3869 show a correlation between line-splitting and line-width similar to that of [OIII]5007 in other studies; and the velocity-splittings are correlated with the quasar Eddington ratio. These results suggest an outfl...

  19. Unprecedented Fine Structure of a Solar Flare Revealed by the 1.6 m New Solar Telescope.

    Science.gov (United States)

    Jing, Ju; Xu, Yan; Cao, Wenda; Liu, Chang; Gary, Dale; Wang, Haimin

    2016-04-13

    Solar flares signify the sudden release of magnetic energy and are sources of so called space weather. The fine structures (below 500 km) of flares are rarely observed and are accessible to only a few instruments world-wide. Here we present observation of a solar flare using exceptionally high resolution images from the 1.6 m New Solar Telescope (NST) equipped with high order adaptive optics at Big Bear Solar Observatory (BBSO). The observation reveals the process of the flare in unprecedented detail, including the flare ribbon propagating across the sunspots, coronal rain (made of condensing plasma) streaming down along the post-flare loops, and the chromosphere's response to the impact of coronal rain, showing fine-scale brightenings at the footpoints of the falling plasma. Taking advantage of the resolving power of the NST, we measure the cross-sectional widths of flare ribbons, post-flare loops and footpoint brighenings, which generally lie in the range of 80-200 km, well below the resolution of most current instruments used for flare studies. Confining the scale of such fine structure provides an essential piece of information in modeling the energy transport mechanism of flares, which is an important issue in solar and plasma physics.

  20. Cell-mediated immunity to HIV-1 in Walter Reed stages 1-6 individuals: correlation with virus burden.

    Science.gov (United States)

    Trauger, R J; Giermakowska, W K; Ferre, F; Duffy, P C; Wallace, M R; Lewis, D E; Beecham, H J; Burnett, K G; Jensen, F C; Carlo, D J

    1993-01-01

    Cell-mediated immunity (CMI) to human immunodeficiency virus-1 (HIV-1) was assessed in a blinded fashion for a patient group (n = 79) representing Walter Reed (WR) stages 1-6. At the same time, viral load was quantitatively measured by two different methods, specifically, virus isolation and HIV viral DNA copy number as measured by the polymerase chain reaction (PCR). After unblinding it was determined that the ability to generate a lymphoproliferative response to an inactivated gp120-depleted HIV (HIV-ag) and tetanus toxoid diminished with advancing WR staging, with complete anergy to HIV-ag and tetanus at stage 6. As a group, individuals whose peripheral blood mononuclear cells (PBMC) proliferated to HIV-ag were either virus isolation negative or produced low levels of virus as measured by p24 antigen (< 250 pg p24) on day 7. Similarly, HIV DNA copy number in the HIV-ag responders was low (< 200 copies/4 x 10(5) PBMC). In contrast, antigen proliferation to tetanus toxoid did not correlate with virus load. Thus, clinical progression as defined by the WR staging system appears to coincide with a loss of CMI to HIV. More importantly, the low viral load measured in HIV-ag responders suggests a link between viral burden and CMI to HIV which might be exploited in the design of immunotherapies for HIV-infected individuals. PMID:8495981

  1. Activation of the fructose 1,6-bisphosphatase gene by 1,25-dihydroxyvitamin D sub 3 during monocytic differentiation

    Energy Technology Data Exchange (ETDEWEB)

    Solomon, D.H.; Raynal, M.C.; Tejwani, G.A.; Cayre, Y.E. (Cornell Univ. Graduate School of Medical Sciences, New York, NY (USA))

    1988-09-01

    Cells from the human leukemia cell line HL-60 undergo terminal monocyte-like differentiation after exposure to either the active circulating form of vitamin D{sub 3}, 1,25-dihydroxyvitamin D{sub 3} (1,25-(OH){sub 2}D{sub 3}), or phorbol 12-myristate 13-acetate. Little is known about the genes that regulate monocytic differentiation. Using clonal variant cells of HL-60 origin, the authors constructed a cDNA library enriched for genes that are induced by 1,25-(OH){sub 2}D{sub 3}. They report that in HL-60, the fructose 1,6-bisphosphatase gene is activated during 1,25-(OH){sub 2}D{sub 3}-induced monocytic differentiation. This gene encodes two closely related mRNAs; one, activated by 1,25-(OH){sub 2}D{sub 3} at an early stage of HL-60 differentiation, encodes a protein that has homology to mammalian FBPase, a key enzyme in gluconeogenesis, although it does not exhibit its classical enzymatic activity. A second mRNA is activated by 1,25-(OH){sub 2}D{sub 3} mainly in peripheral blood monocytes. This mRNA is present in kidney as a unique transcript and encodes a protein with FBPase activity. The data also show that this FBPase-encoding mRNA can be activated during monocytic maturation since it was detected in human alveolar macrophages.

  2. Structural and magnetic properties of FeMnx chains (x =1 -6 ) supported on Cu2N /Cu (100)

    Science.gov (United States)

    Choi, Deung-Jang; Robles, Roberto; Gauyacq, Jean-Pierre; Ternes, Markus; Loth, Sebastian; Lorente, Nicolás

    2016-08-01

    Heterogeneous atomic magnetic chains are built by atom manipulation on a Cu2N /Cu (100) substrate. Their magnetic properties are studied and rationalized by a combined scanning tunneling microscopy (STM) and density functional theory (DFT) work completed by model Hamiltonian studies. The chains are built using Fe and Mn atoms ontop of the Cu atoms along the N rows of the Cu2N surface. Here, we present results for FeMnx chains (x =1 -6 ) emphasizing the evolution of the geometrical, electronic, and magnetic properties with chain size. By fitting our results to a Heisenberg Hamiltonian we have studied the exchange-coupling matrix elements J for different chains. For the shorter chains, x ≤2 , we have included spin-orbit effects in the DFT calculations, extracting the magnetic anisotropy energy. Our results are also fitted to a simple anisotropic spin Hamiltonian and we have extracted values for the longitudinal-anisotropy D and transversal-anisotropy E constants. These parameters together with the values for J allow us to compute the magnetic excitation energies of the system and to compare them with the experimental data.

  3. MULTIWAVELENGTH OBSERVATIONS OF A SLOW-RISE, MULTISTEP X1.6 FLARE AND THE ASSOCIATED ERUPTION

    Energy Technology Data Exchange (ETDEWEB)

    Yurchyshyn, V. [Big Bear Solar Observatory, New Jersey Institute of Technology, Big Bear City, CA 92314 (United States); Kumar, P.; Cho, K.-S.; Lim, E.-K. [Korea Astronomy and Space Science Institute, 776 Daedeok-daero, Yuseong-gu, Daejeon, 305-348 (Korea, Republic of); Abramenko, V. I. [Central Astronomical Observatory of the Russian Academy of Sciences at Pulkovo, 196140, Pulkovskoye chaussee 65, Saint-Petersburg (Russian Federation)

    2015-10-20

    Using multiwavelength observations, we studied a slow-rise, multistep X1.6 flare that began on 2014 November 7 as a localized eruption of core fields inside a δ-sunspot and later engulfed the entire active region (AR). This flare event was associated with formation of two systems of post-eruption arcades (PEAs) and several J-shaped flare ribbons showing extremely fine details, irreversible changes in the photospheric magnetic fields, and it was accompanied by a fast and wide coronal mass ejection. Data from the Solar Dynamics Observatory and IRIS spacecraft, along with the ground-based data from the New Solar Telescope, present evidence that (i) the flare and the eruption were directly triggered by a flux emergence that occurred inside a δ-sunspot at the boundary between two umbrae; (ii) this event represented an example of the formation of an unstable flux rope observed only in hot AIA channels (131 and 94 Å) and LASCO C2 coronagraph images; (iii) the global PEA spanned the entire AR and was due to global-scale reconnection occurring at heights of about one solar radius, indicating the global spatial and temporal scale of the eruption.

  4. CANDELS Observations of the Environmental Dependence of the Color-Mass-Morphology Relation at z = 1.6

    CERN Document Server

    Bassett, Robert; Lotz, Jennifer M; Bell, Eric F; Finkelstein, Steven L; Newman, Jeffrey A; Tran, Kim-Vy; Almaini, Omar; Lani, Caterina; Cooper, Michael; Croton, Darren; Dekel, Avishai; Ferguson, Henry C; Kocevski, Dale D; Koekemoer, Anton M; Koo, David C; McGrath, Elizabeth J; McIntosh, Daniel H; Wechsler, Risa H

    2013-01-01

    We study the environmental dependence of color, stellar mass, and morphology by comparing galaxies in a forming cluster to those in the field at z = 1:6 with Hubble Space Telescope near-infrared imaging in the CANDELS/UDS field. We quantify the morphology of the galaxies using the effective radius, reff, and S\\'ersic index, n. In both the cluster and field, approximately half of the bulge-dominated galaxies (n > 2) reside on the red sequence of the color-magnitude diagram, and most disk-dominated galaxies (n < 2) have colors expected for star-forming galaxies. There is weak evidence that cluster galaxies have redder rest-frame U - B colors and higher stellar masses compared to the field. Star-forming galaxies in both the cluster and field show no significant differences in their morphologies. In contrast, there is evidence that quiescent galaxies in the cluster have larger median effective radii and smaller S\\'ersic indices compared to the field with a significance of 2?. These differences are most pronoun...

  5. PRELIMINARY STUDIES OF SYNTHESIS POLYURETHANE MEMBRANE OF NYAMPLUNG SEED OIL (Calophyllum inophyllum WITH HEXAMETHILENE-1,6-DIISOCYANATE (HMDI

    Directory of Open Access Journals (Sweden)

    Mutia Farida

    2016-09-01

    Full Text Available Nyamplung oil originated from Ujong Pancu, Aceh Besar is one of the vegetable oil sources which is not fully utilized yet. The extraction of nyamplung oil is done using soxhletation with n-hexane solvent; during this process, the oils produced is at the 55.55% levels. The product is in the form of yellowish green liquid with the density of 0.88gr/ml. The nyamplung oil extract has the iodine number of iod 83.53 mg iod/g and hydroxyl number of  64.292 mg KOH/gram which can be utilized as the raw material in making polyurethane membrane. Polyurethane membrane is synthesized by reacting fatty acids from nyamplung seed oil with hexamethylene-1.6-diisocyanate (HDMI. The ration of the oil towards HDMI is variated as, 5:1;5:3;5:5;5:7;5:9 v/w. The membrane has a composition of  5:7 v/w  with 90-100 oC polymerization temperatures and  160oC curring temperature for 8 hours is hard, homogenous and quite elastic with a transparent brown colour.

  6. The 1.6 Å resolution structure of a FRET-optimized Cerulean fluorescent protein

    Science.gov (United States)

    Watkins, Jennifer L.; Kim, Hanseong; Markwardt, Michele L.; Chen, Liqing; Fromme, Raimund; Rizzo, Mark A.; Wachter, Rebekka M.

    2013-01-01

    Genetically encoded cyan fluorescent proteins (CFPs) bearing a tryptophan-derived chromophore are commonly used as energy-donor probes in Förster resonance energy transfer (FRET) experiments useful in live cell-imaging applications. In recent years, significant effort has been expended on eliminating the structural and excited-state heterogeneity of these proteins, which has been linked to undesirable photophysical properties. Recently, mCerulean3, a descendant of enhanced CFP, was introduced as an optimized FRET donor protein with a superior quantum yield of 0.87. Here, the 1.6 Å resolution X-ray structure of mCerulean3 is reported. The chromophore is shown to adopt a planar trans configuration at low pH values, indicating that the acid-induced isomerization of Cerulean has been eliminated. β-Strand 7 appears to be well ordered in a single conformation, indicating a loss of conformational heterogeneity in the vicinity of the chromophore. Although the side chains of Ile146 and Leu167 appear to exist in two rotamer states, they are found to be well packed against the indole group of the chromophore. The Ser65 reversion mutation allows improved side-chain packing of Leu220. A structural comparison with mTurquoise2 is presented and additional engineering strategies are discussed. PMID:23633585

  7. ULTRA-NARROW NEGATIVE FLARE FRONT OBSERVED IN HELIUM-10830 Å USING THE 1.6 m NEW SOLAR TELESCOPE

    Energy Technology Data Exchange (ETDEWEB)

    Xu, Yan; Liu, Chang; Jing, Ju; Wang, Haimin [Space Weather Research Lab, Center for Solar-Terrestrial Research, New Jersey Institute of Technology, 323 Martin Luther King Blvd, Newark, NJ 07102-1982 (United States); Cao, Wenda; Gary, Dale [Big Bear Solar Observatory, New Jersey Institute of Technology 323 Martin Luther King Blvd, Newark, NJ 07102-1982 (United States); Ding, Mingde [School of Astronomy and Space Science, Nanjing University, Nanjing 210093 (China); Kleint, Lucia [Fachhochschule Nordwestschweiz (FHNW), Institute of 4D technologies Bahnhofstr. 6, CH-5210 Windisch (Switzerland); Su, Jiangtao [Key Laboratory of Solar Activity, National Astronomical Observatories, Chinese Academy of Sciences, Beijing 100012 (China); Ji, Haisheng [Purple Mountain Observatory, 2 Beijing Xi Lu, Nanjing, 210008 (China); Chae, Jongchul; Cho, Kyuhyoun [Astronomy Program, Department of Physics and Astronomy, Seoul National University, Seoul 151-747 (Korea, Republic of); Cho, Kyungsuk [Korea Astronomy and Space Science Institute, Daedeokdae-ro 776, Yuseong-gu, Daejeon 305-348 (Korea, Republic of)

    2016-03-10

    Solar flares are sudden flashes of brightness on the Sun and are often associated with coronal mass ejections and solar energetic particles that have adverse effects on the near-Earth environment. By definition, flares are usually referred to as bright features resulting from excess emission. Using the newly commissioned 1.6 m New Solar Telescope at Big Bear Solar Observatory, we show a striking “negative” flare with a narrow but unambiguous “dark” moving front observed in He i 10830 Å, which is as narrow as 340 km and is associated with distinct spectral characteristics in Hα and Mg ii lines. Theoretically, such negative contrast in He i 10830 Å can be produced under special circumstances by nonthermal electron collisions or photoionization followed by recombination. Our discovery, made possible due to unprecedented spatial resolution, confirms the presence of the required plasma conditions and provides unique information in understanding the energy release and radiative transfer in astronomical objects.

  8. cpFBPaseII, a novel redox-independent chloroplastic isoform of fructose-1,6-bisphosphatase.

    Science.gov (United States)

    Serrato, Antonio Jesús; Yubero-Serrano, Elena María; Sandalio, Luisa María; Muñoz-Blanco, Juan; Chueca, Ana; Caballero, José Luis; Sahrawy, Mariam

    2009-07-01

    A full-length FBPase cDNA has been isolated from Fragaria x ananassa (strawberry) corresponding to a novel putative chloroplastic FBPase but lacking the regulatory redox domain, a characteristic of the plastidial isoenzyme (cpFBPaseI). Another outstanding feature of this novel isoform, called cpFBPaseII, is the absence of the canonical active site. Enzymatic assays with cpFBPaseII evidenced clear Mg(2+)-dependent FBPase activity and a K(m) for fructose-1,6-bisphosphate (FBP) of 1.3 mM. Immunolocalization experiments and chloroplast isolation confirmed that the new isoenzyme is located in the stroma. Nevertheless, unlike cpFBPaseI, which is redox activated, cpFBPaseII did not increase its activity in the presence of either DTT or thioredoxin f (TRX f) and is resistant to H(2)O(2) inactivation. Additionally, the novel isoform was able to complement the growth deficiency of the yeast FBP1 deletion fed with a non-fermentable carbon source. Furthermore, orthologues are restricted to land plants, suggesting that cpFBPaseII is a novel and an intriguing chloroplastic FBPase that emerged late in the evolution of photosynthetic organisms, possibly because of a pressing need of land plants.

  9. Ionization energies of three resonance-stabilized radicals: cyclohexadienyl (dn, n = 0, 1, 6, 7), 1-phenylpropargyl, and methylcyclohexadienyl.

    Science.gov (United States)

    Krechkivska, Olha; Wilcox, Callan; O'Connor, Gerard D; Nauta, Klaas; Kable, Scott H; Schmidt, Timothy W

    2014-11-06

    The ionization energies for three resonance-stabilized radicals are determined: cyclohexadienyl, 1-phenylpropargyl, and methylcyclohexadienyl. The recommended ionization energies are, respectively, 6.820(1), 6.585(1), and 7.232(1) eV. That of cyclohexadienyl is found to be just 0.02 eV above a high level ab initio calculation [Bargholz, A.; Oswald, R.; Botschwina, P. J. Chem. Phys. 2013, 138, 014307], and that of 1-phenylpropargyl is found within the stated error of a recent experimental determination [Holzmeier, F.; Lang, M.; Hemberger, P.; Fischer, I. ChemPhysChem 2014, DOI: 10.1002/cphc.201402446]. The ionization energy of the methylcyclohexadienyl radical is consistent with the ortho isomer. Ionization energies of a range of isotopologues of cyclohexadienyl radical are given, along with their D1 ← D0 origin band positions, which indicate a blue shift of 18 cm(-1) per deuterium atom substituted. The ionization energy of cyclohexadienyl, along with the calculated bond dissociation energy of Bargholz et al., affords a new estimate of the 0 K proton affinity of benzene: 739.7 ± 2.0 kJ/mol. The ionization energies are discussed in terms of the interplay between radical and cation stabilization energies.

  10. Volume Regulated Channels

    DEFF Research Database (Denmark)

    Klausen, Thomas Kjær

    - serves a multitude of functions in the mammalian cell, regulating the membrane potential (Em), cell volume, protein activity and the driving force for facilitated transporters giving Cl- and Cl- channels a major potential of regulating cellular function. These functions include control of the cell cycle...... of volume perturbations evolution have developed system of channels and transporters to tightly control volume homeostasis. In the past decades evidence has been mounting, that the importance of these volume regulated channels and transporters are not restricted to the defense of cellular volume......, controlled cell death and cellular migration. Volume regulatory mechanisms has long been in focus for regulating cellular proliferation and my thesis work have been focusing on the role of Cl- channels in proliferation with specific emphasis on ICl, swell. Pharmacological blockage of the ubiquitously...

  11. Precision volume measurement system.

    Energy Technology Data Exchange (ETDEWEB)

    Fischer, Erin E.; Shugard, Andrew D.

    2004-11-01

    A new precision volume measurement system based on a Kansas City Plant (KCP) design was built to support the volume measurement needs of the Gas Transfer Systems (GTS) department at Sandia National Labs (SNL) in California. An engineering study was undertaken to verify or refute KCP's claims of 0.5% accuracy. The study assesses the accuracy and precision of the system. The system uses the ideal gas law and precise pressure measurements (of low-pressure helium) in a temperature and computer controlled environment to ratio a known volume to an unknown volume.

  12. Ultramassive dense early-type galaxies: Velocity dispersions and number density evolution since z = 1.6

    Science.gov (United States)

    Gargiulo, A.; Saracco, P.; Tamburri, S.; Lonoce, I.; Ciocca, F.

    2016-08-01

    Aims: We investigate the stellar mass assembly history of ultramassive (M⋆ ≳ 1011M⊙) dense (Σ = M⋆/2πRe2> 2500M⊙ pc-2) early-type galaxies (ETGs, elliptical and spheroidal galaxies) selected on basis of visual classification over the last 9 Gyr. Methods: We traced the evolution of the comoving number density ρ of ultramassive dense ETGs and compared their structural (effective radius Re and stellar mass M⋆) and dynamical (velocity dispersion σe) parameters over the redshift range 0 advantage of the COSMOS spectroscopic survey to probe the intermediate redshift range [0.2-1.0]. We derived the number density of ultramassive dense local ETGs from the SDSS sample taking all of the selection bias affecting the spectroscopic sample into account. To compare the dynamical and structural parameters, we collected a sample of 11 ultramassive dense ETGs at 1.2 measurements are available. For four of these ETGs (plus one at z = 1.91), we present previously unpublished estimates of velocity dispersion, based on optical VLT-FORS2 spectra. We probe the intermediate redshift range (0.2 ≲ z ≲ 0.9) and the local Universe with different ETGs samples. Results: We find that the comoving number density of ultramassive dense ETGs evolves with z as ρ(z) ∝ (1 + z)0.3 ± 0.8 implying a decrease of ~25% of the population of ultramassive dense ETGs since z = 1.6. By comparing the structural and dynamical properties of high-z ultramassive dense ETGs over the range 0 ≲ z < 1.6 in the [Re, M⋆, σe] plane, we find that all of the ETGs of the high-z sample have counterparts with similar properties in the local Universe. This implies either that the majority (~70%) of ultramassive dense ETGs already completed the assembly and shaping at ⟨ z ⟩ = 1.4, or that, if a significant portion of dense ETGs evolves in size, new ultramassive dense ETGs must form at z < 1.5 to maintain their number density at almost constant. The difficulty in identify good progenitors for these

  13. Gripe Water Administration in Infants 1-6 months of Age-A Cross-sectional Study.

    Science.gov (United States)

    Jain, Keerthi; Gunasekaran, Dhandapany; Venkatesh, Chandrasekaran; Soundararajan, Palanisamy

    2015-11-01

    Gripe water (GW) administration to young infants is common practice in this part of country. In order to ascertain why mothers administer gripe water to their infants and to find out what benefits or health risks it poses, we proposed to study the practice of mothers giving GW to their babies. Three hundred and thirty five eligible mothers of infants aged 1-6 months (who after qualifying inclusion and exclusion criteria of the study) who attended the well baby clinic during the study period, were interviewed using a semi structured questionnaire which contained both open and close ended questions after obtaining informed written consent. The study population was then divided into two groups based on administration of GW or not and the results were compared and analysed among the two groups using odds ratio with 95% C.I. For calculation of statistics, the statistical package SPSS 13 was used. 64.18% of the mothers were administering GW for their infants. Most mothers believed that GW helps in digestion and prevents stomach ache. Infantile colic, vomiting and constipation were common in GW administered infants, when compared to those who did not receive GW and the difference was significant with p-values of 0.0001, 0.0373, 0.0007respectively. GW administration is a common problem in infants and remains a significant challenge that thwarts exclusive breast feeding. More over GW administration does not seem to prevent infantile colic and on the other hand, may be associated with vomiting and constipation. Misconceptions prevailing among mothers have to be removed by effective counseling so that the mothers are aware of safe and healthy feeding practices to be adopted for feeding their babies.

  14. Medial hamstring muscle activation patterns are affected 1-6 years after ACL reconstruction using hamstring autograft.

    Science.gov (United States)

    Arnason, Stefán Magni; Birnir, Bjartmar; Guðmundsson, Tómas Emil; Guðnason, Garðar; Briem, Kristín

    2014-05-01

    Although changes in hamstring muscle morphology after anterior cruciate ligament reconstruction (ACLR) using a semitendinosus autograft hamstrings-gracilis (HG) of the ipsilateral limb are recognized, alterations in muscle activation patterns have not been extensively studied. The purpose of this controlled laboratory trial was therefore to monitor muscle activation levels of the medial (MH) and lateral (LH) hamstring muscles in athletes who had undergone ACLR using a HG autograft and to contrast these to activation levels demonstrated by healthy controls. Surface electromyography (EMG) was sampled from bilateral hamstring muscles of 18 athletes 1-6 years after ACLR and 18 matched controls (CTRL) during the performance of two dissimilar exercises, both involving eccentric knee flexor activity. Peak normalized muscle activation levels were identified for MH and LH of both limbs during the performance of the Nordic Hamstring (NH) exercise and TRX) hamstring curl (TRX) exercise. A statistically significant limb by exercise interaction was found for peak activation levels of LH, due to significant interlimb differences in activation during the performance of the TRX exercise compared to more symmetrical activation during the NH (p exercise type (p = 0.025). Whereas CTRL group participants consistently favoured one limb over the other during the performance of both exercises, ACLR participants demonstrated dissimilar peak MH activation patterns between limbs during the performance of the NH exercise compared to the TRX. In light of these results and considering the surgical procedure, patients who undergo ACLR using a HG autograft from the ipsilateral limb may benefit from post-operative rehabilitation that involves muscle activation and strengthening specifically targeting the MH component.

  15. CANDELS OBSERVATIONS OF THE ENVIRONMENTAL DEPENDENCE OF THE COLOR-MASS-MORPHOLOGY RELATION AT z = 1.6

    Energy Technology Data Exchange (ETDEWEB)

    Bassett, Robert; Papovich, Casey; Tran, Kim-Vy [George P. and Cynthia Woods Mitchell Institute for Fundamental Physics and Astronomy, College Station, TX 77843-4242 (United States); Lotz, Jennifer M.; Ferguson, Henry C.; Koekemoer, Anton M. [Space Telescope Science Institute, 3700 San Martin Dr., Baltimore, MD 21218 (United States); Bell, Eric F. [Department of Astronomy, University of Michigan, Ann Arbor, MI 48109 (United States); Finkelstein, Steven L. [Department of Astronomy, University of Texas, Austin, TX 78712 (United States); Newman, Jeffrey A. [Department of Physics and Astronomy, University of Pittsburgh, Pittsburgh, PA 15260 (United States); Almaini, Omar; Lani, Caterina [School of Physics and Astronomy, University of Nottingham, Nottingham (United Kingdom); Cooper, Michael [Center for Galaxy Evolution, Department of Physics and Astronomy, University of California, Irvine, 4129 Frederick Reines Hall, Irvine, CA 92697 (United States); Croton, Darren [Centre for Astrophysics and Supercomputing, Swinburne University of Technology, Hawthorn (Australia); Dekel, Avishai [Racah Institute of Physics, The Hebrew University, Jerusalem 91904 (Israel); Kocevski, Dale D.; Koo, David C.; McGrath, Elizabeth J. [UCO/Lick Observatory, Department of Astronomy and Astrophysics, University of California, Santa Cruz, CA 95064 (United States); McIntosh, Daniel H. [Department of Physics, University of Missouri-Kansas City, 5110 Rockhill Road, Kansas City, MO 64110 (United States); Wechsler, Risa [Kavli Institute for Particle Astrophysics and Cosmology, Physics Department, and SLAC National Accelerator Laboratory, Stanford University, Stanford, CA 94305 (United States)

    2013-06-10

    We study the environmental dependence of color, stellar mass, and morphology by comparing galaxies in a forming cluster to those in the field at z = 1.6 with Hubble Space Telescope near-infrared imaging in the CANDELS/UDS field. We quantify the morphology of the galaxies using the effective radius, r{sub eff}, and Sersic index, n. In both the cluster and field, approximately half of the bulge-dominated galaxies (n > 2) reside on the red sequence of the color-magnitude diagram, and most disk-dominated galaxies (n < 2) have colors expected for star-forming galaxies. There is weak evidence that cluster galaxies have redder rest-frame U - B colors and higher stellar masses compared to the field. Star-forming galaxies in both the cluster and field show no significant differences in their morphologies. In contrast, there is evidence that quiescent galaxies in the cluster have larger median effective radii and smaller Sersic indices compared to the field with a significance of 2{sigma}. These differences are most pronounced for galaxies at clustercentric distances 1 Mpc

  16. KMTNET: A Network of 1.6 m Wide-Field Optical Telescopes Installed at Three Southern Observatories

    Science.gov (United States)

    Kim, Seung-Lee; Lee, Chung-Uk; Park, Byeong-Gon; Kim, Dong-Jin; Cha, Sang-Mok; Lee, Yongseok; Han, Cheongho; Chun, Moo-Young; Yuk, Insoo

    2016-02-01

    The Korea Microlensing Telescope Network (KMTNet) is a wide-field photometric system installed by the Korea Astronomy and Space Science Institute (KASI). Here, we present the overall technical specifications of the KMTNet observation system, test observation results, data transfer and image processing procedure, and finally, the KMTNet science programs. The system consists of three 1.6 m wide-field optical telescopes equipped with mosaic CCD cameras of 18k by 18k pixels. Each telescope provides a 2.0 by 2.0 square degree field of view. We have finished installing all three telescopes and cameras sequentially at the Cerro-Tololo Inter-American Observatory (CTIO) in Chile, the South African Astronomical Observatory (SAAO) in South Africa, and the Siding Spring Observatory (SSO) in Australia. This network of telescopes, which is spread over three different continents at a similar latitude of about -30 degrees, enables 24-hour continuous monitoring of targets observable in the Southern Hemisphere. The test observations showed good image quality that meets the seeing requirement of less than 1.0 arcsec in I-band. All of the observation data are transferred to the KMTNet data center at KASI via the international network communication and are processed with the KMTNet data pipeline. The primary scientific goal of the KMTNet is to discover numerous extrasolar planets toward the Galactic bulge by using the gravitational microlensing technique, especially earth-mass planets in the habitable zone. During the non-bulge season, the system is used for wide-field photometric survey science on supernovae, asteroids, and external galaxies.

  17. KMTNet: a network of 1.6-m wide field optical telescopes installed at three southern observatories

    Science.gov (United States)

    Lee, Chung-Uk; Kim, Seung-Lee; Cha, Sang-Mok; Lee, Yongseok; Kim, Dong-Jin; Park, Byeong-Gon; Lee, Dong-Joo; Koo, Jae-Rim; Hong, Kyeongsoo; Lee, Jae Woo; Ryu, Yoon-Hyun; Lim, Beomdu; Lim, Jin-Sun; Gho, Seung-Won; Kim, Min-Jun

    2015-08-01

    Korea Astronomy and Space Science Institute (KASI) have installed three identical 1.6-m telescopes, called Korea Microlensing Telescope Network (KMTNet), which cover 2 x 2 degree field of view with the plate scale of 0.4 arcsec/pixel at three observatories - CTIO, SSO and SAAO in southern hemisphere. The uniqueness of the system is the uninterupted 24-hour monitoring with a wide field optics in southern hemisphere. The telescope adopts prime focus using a parabolic mirror and four spherical flattening lenses. The structural design and driving systems are modified from the degin of 2MASS telescope. The one piece filter-shutter assembly has a sliding shutter and four 310-mm square filters. Each observation system produces a 680MB size image file at site and the images are transfered to KASI data center using the Global Ring Network for Advanced Application Development (GLORIAD) network with the band width of 50Mbps in average. The main science goal of the KMTNet is to discover Earth like extra solar planet using the microlensing technique during bulge season, and 50% of the total observation time is allocated for the science program solely. The other telescope times are allocated for pre-selected seven science programs during non-bulge season. From the test observation, we verify that the most important two requirements are satisfied: 10 arcsec in RMS for the pointing accuracy and 1 arcsec of delivered image quality in I-band. In this presentation, we introduce finally installed system at each observatory and its observational performance obtained from the test observation.

  18. Synthesis and characterization of biodegradable acrylated polyurethane based on poly(ε-caprolactone) and 1,6-hexamethylene diisocyanate

    Energy Technology Data Exchange (ETDEWEB)

    Alishiri, M. [Department of Chemical and Petroleum Engineering, Sharif University of Technology, P. O. Box 11155-9465, Tehran (Iran, Islamic Republic of); Shojaei, A., E-mail: akbar.shojaei@sharif.edu [Department of Chemical and Petroleum Engineering, Sharif University of Technology, P. O. Box 11155-9465, Tehran (Iran, Islamic Republic of); Abdekhodaie, M.J. [Department of Chemical and Petroleum Engineering, Sharif University of Technology, P. O. Box 11155-9465, Tehran (Iran, Islamic Republic of); Yeganeh, H. [Department of Polyurethane, Iran Polymer and Petrochemical Institute, P.O. Box 14965/115, Tehran (Iran, Islamic Republic of)

    2014-09-01

    A series of biodegradable acrylic terminated polyurethanes (APUs) based on poly(ε-caprolactone) diol (PCL), aliphatic 1,6-hexamethylene diisocyanate (HDI) and hydroxyethyl methyl acrylate (HEMA) was synthesized as potential materials for hard tissue biomedical applications. PCLs with low molecular weights of 1000 and 2000 g/mol were employed to provide different amounts of end capped urethane acrylate in APUs. To control crosslink density, a mixture of two different reactive diluents including mono-functional HEMA and bi-functional ethylene glycol dimethacrylate (EGDMA) with different weight ratios was incorporated into the APUs, called here PUAs. Morphological characteristics and mechanical properties were investigated using X-ray diffraction (XRD), differential scanning calorimetry (DSC) and dynamic mechanical analysis (DMA). DMA results indicated some degree of microphase separation between hard and soft segments; however, the microphase separation is more prominent for PUAs with higher molecular weight PCL. It was also found that the degree of crosslinking dominated greatly the formation of crystalline structure. PUAs with low crosslink density exhibited crystalline microstructure. The results also indicated that the mechanical properties of PUAs were governed considerably by crystalline microstructure, and hard segment content. All PUAs demonstrated hydrophobic behavior and were able to be degraded hydrolytically. The degradation process was closely related to the microstructure and surface tension of PUAs. - Highlights: • We synthesized biodegradable acrylated polyurethanes (PUA) based on PCL and HDI. • Morphological and mechanical properties were investigated experimentally. • Increasing crystallinity and hard segment content enhances the mechanical properties. • Hydrolytic degradation was associated with surface tension and amorphous structure.

  19. Water-soluble low-molecular-weight -(1, 3–1, 6 D-Glucan inhibit cedar pollinosis

    Directory of Open Access Journals (Sweden)

    Tomoko Jippo

    2015-02-01

    Full Text Available Background: The incidence of allergic diseases such as allergic rhinitis, atopic dermatitis, asthma, and food allergies has increased in several countries. Mast cells have critical roles in various biologic processes related to allergic diseases. Mast cells express the high-affinity receptor for immunoglobulin (Ig E on their surface. The interaction of multivalent antigens with surface-bound IgE causes the secretion of granule-stored mediators, as well as the de novosynthesis of cytokines. Those mediators and cytokines proceed the allergic diseases. We investigated the effects of water-soluble, low-molecular-weight -(1, 3–1, 6 D-glucan isolated from Aureobasidium pullulans 1A1 strain black yeast (LMW--glucan on mast cell-mediated anaphylactic reactions. We reported that LMW--glucan dose-dependently inhibited the degranulation of mast cells. Furthermore, we found that orally administered LMW--glucan inhibited the IgE-mediated passive cutaneous anaphylaxis (PCA reaction in mice. Here, we examined if LMW--glucan had effects on Japanese cedar pollinosis. Findings: In a clinical study, a randomized, single-blind, placebo-controlled, parallel group study in 65 subjects (aged 2262 was performed. This study was undertaken 3 weeks before and until the end of the cedar pollen season. During the study, all subjects consumed one bottle of placebo or LMW--glucan daily and all subjects were required to record allergic symptoms in a diary. The LMW--glucan group had a significantly lower prevalence of sneezing, nose-blowing, tears, and hindrance to the activities of daily living than the placebo group. Conclusions: These results suggested that LMW--glucan could be an effective treatment for allergic diseases

  20. Quadruple space-group ambiguity owing to rotational and translational noncrystallographic symmetry in human liver fructose-1,6-bisphosphatase.

    Science.gov (United States)

    Ruf, Armin; Tetaz, Tim; Schott, Brigitte; Joseph, Catherine; Rudolph, Markus G

    2016-11-01

    Fructose-1,6-bisphosphatase (FBPase) is a key regulator of gluconeogenesis and a potential drug target for type 2 diabetes. FBPase is a homotetramer of 222 symmetry with a major and a minor dimer interface. The dimers connected via the minor interface can rotate with respect to each other, leading to the inactive T-state and active R-state conformations of FBPase. Here, the first crystal structure of human liver FBPase in the R-state conformation is presented, determined at a resolution of 2.2 Å in a tetragonal setting that exhibits an unusual arrangement of noncrystallographic symmetry (NCS) elements. Self-Patterson function analysis and various intensity statistics revealed the presence of pseudo-translation and the absence of twinning. The space group is P41212, but structure determination was also possible in space groups P43212, P4122 and P4322. All solutions have the same arrangement of three C2-symmetric dimers spaced by 1/3 along an NCS axis parallel to the c axis located at (1/4, 1/4, z), which is therefore invisible in a self-rotation function analysis. The solutions in the four space groups are related to one another and emulate a body-centred lattice. If all NCS elements were crystallographic, the space group would be I4122 with a c axis three times shorter and a single FBPase subunit in the asymmetric unit. I4122 is a minimal, non-isomorphic supergroup of the four primitive tetragonal space groups, explaining the space-group ambiguity for this crystal.

  1. IDENTIFICATION OF OUTFLOWS AND CANDIDATE DUAL ACTIVE GALACTIC NUCLEI IN SDSS QUASARS AT z = 0.8-1.6

    Energy Technology Data Exchange (ETDEWEB)

    Barrows, R. Scott; Lacy, Claud H. Sandberg; Kennefick, Julia; Kennefick, Daniel; Berrier, Joel C. [Arkansas Center for Space and Planetary Sciences, University of Arkansas, Fayetteville, AR 72701 (United States); Comerford, Julia M., E-mail: rbarrows@uark.edu [Astronomy Department, University of Texas at Austin, Austin, TX 78712 (United States)

    2013-06-01

    We present a sample of 131 quasars from the Sloan Digital Sky Survey at redshifts 0.8 < z < 1.6 with double peaks in either of the high-ionization narrow emission lines [Ne V] {lambda}3426 or [Ne III] {lambda}3869. These sources were selected with the intention of identifying high-redshift analogs of the z < 0.8 active galactic nuclei (AGNs) with double-peaked [O III] {lambda}5007 lines, which might represent AGN outflows or dual AGNs. Lines of high ionization potential are believed to originate in the inner, highly photoionized portion of the narrow line region, and we exploit this assumption to investigate the possible kinematic origins of the double-peaked lines. For comparison, we measure the [Ne V] {lambda}3426 and [Ne III] {lambda}3869 double peaks in low-redshift (z < 0.8) [O III]-selected sources. We find that [Ne V] {lambda}3426 and [Ne III] {lambda}3869 show a correlation between line splitting and line width similar to that of [O III] {lambda}5007 in other studies, and the velocity splittings are correlated with the quasar Eddington ratio. These results suggest an outflow origin for at least a subset of the double peaks, allowing us to study the high-ionization gas kinematics around quasars. However, we find that a non-negligible fraction of our sample show no evidence for an ionization stratification. For these sources, the outflow scenario is less compelling, leaving the dual AGN scenario as a viable possibility. Finally, we find that our sample shows an anti-correlation between the velocity-offset ratio and luminosity ratio of the components, which is a potential dynamical argument for the presence of dual AGNs. Therefore, this study serves as a first attempt at extending the selection of candidate dual AGNs to higher redshifts.

  2. Depth dependence of westward-propagating North Atlantic features diagnosed from altimetry and a numerical 1/6° model

    Directory of Open Access Journals (Sweden)

    R. Tailleux

    2008-03-01

    Full Text Available A 1/6° numerical simulation is used to investigate the vertical structure of westward propagation between 1993 and 2000 in the North Atlantic ocean. The realism of the simulated westward propagating signals, interpreted principally as the signature of first-mode baroclinic Rossby waves (RW, is first assessed by comparing the simulated amplitude and zonal phase speeds of Sea Level Anomalies (SLA against TOPEX/Poseidon-ERS satellite altimeter data. Then, the (unobserved subsurface signature of RW phase speeds is investigated from model outputs by means of the Radon Transform which was specifically adapted to focus on first-mode baroclinic RW. The analysis is performed on observed and simulated SLA and along 9 simulated isopycnal displacements spanning the 0–3250 m depth range. Simulated RW phase speeds agree well with their observed counterparts at the surface, although with a slight slow bias. Below the surface, the simulated phase speeds exhibit a systematic deceleration with increasing depth, by a factor that appears to vary geographically. Thus, while the reduction factor is about 15–18% on average at 3250 m over the region considered, it appears to be much weaker (about 5–8% in the eddy-active Azores Current, where westward propagating structures might be more coherent in the vertical. In the context of linear theories, these results question the often-made normal mode assumption of many WKB-based theories that the phase speed is independent of depth. Alternatively, these results could also suggest that the vertical structure of westward propagating signals may significantly depend on their degree of nonlinearity, with the degree of vertical coherence possibly increasing with the degree of nonlinearity.

  3. Characterization of mice with targeted deletion of the gene encoding core 2 beta1,6-N-acetylglucosaminyltransferase-2.

    Science.gov (United States)

    Stone, Erica L; Lee, Seung Ho; Ismail, Mohd Nazri; Fukuda, Minoru

    2010-01-01

    The three glycosyltransferases of the Core 2 beta1,6-N-acetylglucosaminyltransferase (C2GnT) family, C2GnT1, C2GnT2, and C2GnT3, are able to initiate the Core 2 branch of O-glycans. However, C2GnT2, which is highly expressed in the digestive tract, has a broader acceptor substrate specificity that allows it to also generate Core 4 O-glycans and I branches. We discovered that C2GnT2 KO mice have decreased mucosal barrier function in the digestive tract, reduced levels of circulating IgGs and fecal IgA, and increased susceptibility to experimental colitis. Mass spectrometric analyses also revealed that C2GnT2 KO mice had a reduction in Core 2 O-glycans in the digestive tract with a corresponding increase in elongated Core 1 O-glycans. Unexpectedly, we saw that the loss of C2GnT2 and especially the loss of all three C2GnTs resulted in the expression of elongated O-mannose structures in the stomach, suggesting that the elongation of these structures is controlled by competition for UDP-GlcNAc [Stone, E. L., Ismail, M. N., Lee, S. H., Luu, Y., Ramirez, K., Haslam, S. M., Ho, S. B., Dell, A., Fukuda, M. and Marth, J. D. (2009). Glycosyltransferase function in Core 2-type protein O-glycosylation. Mol. Cell. Biol. 29, 3370-3782].

  4. Knee function among elite handball and football players 1-6 years after anterior cruciate ligament injury.

    Science.gov (United States)

    Myklebust, G; Bahr, R; Nilstad, A; Steffen, K

    2017-01-20

    The aim of the study was to describe objective and self-reported knee function for athletes who have returned to elite handball and football play after an ACL injury, comparing these to non-injured players at the same level. A total of 414 handball and 444 football players completed baseline tests from 2007 through 2014, examining lower extremity strength, dynamic balance, knee laxity, and knee function (KOOS questionnaire). Measures were compared between injured and non-injured legs and between injured legs and legs of controls. Eighty (9.3%) of the 858 players reported a previous ACL injury, 1-6 years post-injury (3.5±2.5 years), 49 handball (61.3%) and 31 football players (38.7%). We found no difference in strength or dynamic balance between previously ACL-injured (N=80) and non-injured players legs (N=1556). However, lower quadriceps (6.3%, 95% CI: 3.2-9.2) and hamstrings muscle strength (6.1%, 95% CI: 3.3-8.1) were observed in previously ACL-injured legs compared to the non-injured contralateral side (N=80). ACL-injured knees displayed greater joint laxity than the contralateral knee (N=80, 17%, 95% CI: 8-26) and healthy knees (N=1556, 23%, 95% CI: 14-33). KOOS scores were significantly lower for injured knees compared to knees of non-injured players. ACL-injured players who have successfully returned to elite sport have comparable strength and balance measures as their non-injured teammates. Subjective perception of knee function is strongly affected by injury history, with clinically relevant lower scores for the KOOS subscores Pain, Function, Sport, and Quality Of Life.

  5. Repeated febrile convulsions impair hippocampal neurons and cause synaptic damage in immature rats:neuroprotective effect of fructose-1,6-diphosphate

    Institute of Scientific and Technical Information of China (English)

    Jianping Zhou; Fan Wang; Jun Zhang; Hui Gao; Yufeng Yang; Rongguo Fu

    2014-01-01

    Fructose-1,6-diphosphate is a metabolic intermediate that promotes cell metabolism. We hy-pothesize that fructose-1,6-diphosphate can protect against neuronal damage induced by febrile convulsions. Hot-water bathing was used to establish a repetitive febrile convulsion model in rats aged 21 days, equivalent to 3-5 years in humans. Ninety minutes before each seizure induc-tion, rats received an intraperitoneal injection of low- or high-dose fructose-1,6-diphosphate (500 or 1,000 mg/kg, respectively). Low- and high-dose fructose-1,6-diphosphate prolonged the latency and shortened the duration of seizures. Furthermore, high-dose fructose-1,6-di-phosphate effectively reduced seizure severity. Transmission electron microscopy revealed that 24 hours after the last seizure, high-dose fructose-1,6-diphosphate reduced mitochondrial swelling, rough endoplasmic reticulum degranulation, Golgi dilation and synaptic cleft size, and increased synaptic active zone length, postsynaptic density thickness, and synaptic interface cur-vature in the hippocampal CA1 area. The present findings suggest that fructose-1,6-diphosphate is a neuroprotectant against hippocampal neuron and synapse damage induced by repeated fe-brile convulsion in immature rats.

  6. Analysis of Genetic Polymorphism in DongGuan Randomized Donors' HPA 1-6 Antigens%现居东莞人群人类血小板1-6抗原系统基因分型研究

    Institute of Scientific and Technical Information of China (English)

    师玲玲; 王德文; 刘赴平

    2009-01-01

    目的 研究现居东莞人群人类血小板抗原(human Platelet antigen,HAP)1-6系统基因分型及其多态性分布特征.方法 用DNA提取试剂盒提取外周血标本中的DNA,用聚合酶链式反应-序列特异性引物(PCR-SSP)扩增HPA等位基因.结果 80名健康、无血缘关系的现居东莞人群每个样本均检测到HPA-1a、2a、4a、5a、6a基因;HPA-4a、5a呈现纯合子单态性,未检测出相应的等位基因HPA-b;对于HPA-1、-2、-6主要以a/a纯合子为多,a/a基因型频率分别是0.975、0.725、0.9625,没有b/b纯合子出现.结论 HPA血型系统多态性具有人群特征.与其他种族相比,现居东莞人群HPA血型系统中a基因频率较高,b基因频率较低,提示在现居东莞人群中由b基因不合所引起的同种免疫病理反应要低于其他种族人群.现居东莞人群HPA-1、-2、-3、-6系统具有多态性,HPA-2、-3抗原杂和率比较高,提示在临床输血实践中可能具有重要的免疫学意义.同时,在此次研究数据的基础上建立了现居东莞人群血小板基因频率数据库和血小板基因型已知供者库.%Objective To characterize the allele frequencies and its polymorphisms of human platelet 1-6 antigen (HPA) in DongGuan population. Methods The genomic DNA of 80 healthy and unrelated individuals from blood donors was extracted from the peripheral blood samples with DNA extraction kit reagent and genotyped for by polymerase chain reaction with sequence specific primers (PCR-SSP). Results HPA-1a, 2a, 4a, 5a ,6a alleles were found in each of the samples. HPA-4a, 5a all showed homozygous genotype with no responsive HPA-b alleles were found. HPA-1, -2, -6 mainly showed a/a homozygous, the frequencies of homozygous HPA-1a/a, 2a/a, 6a/a were 0.975, 0.725, 0.9625 respectively, and homozygous (b/b) were not detected. Conclusion Polymorphism of HPA has differential population characteristic. Compared with other populations, population in DongGuan displayed higher

  7. Glassy nature of stripe ordering in La1.6-xNd0.4SrxCuO4

    Science.gov (United States)

    Tranquada, J. M.; Ichikawa, N.; Uchida, S.

    1999-06-01

    We present the results of neutron-scattering studies on various aspects of crystalline and magnetic structure in single crystals of La1.6-xNd0.4SrxCuO4 with x=0.12 and 0.15. In particular, we have reexamined the degree of stripe order in an x=0.12 sample. Measurements of the width for an elastic magnetic peak show that it saturates at a finite value below 30 K, corresponding to a spin-spin correlation length of 200 Å . A model calculation indicates that the differing widths of magnetic and (previously reported) charge-order peaks, together with the lack of commensurability, can be consistently explained by disorder in the stripe spacing. Above 30 K (i.e., above the point at which a recent muon spin-rotation study has found a loss of static magnetic order), the width of the nominally elastic signal begins to increase. Interpreting the signal as critical scattering from slowly fluctuating spins, the temperature dependence of the width is consistent with renormalized classical behavior of a two-dimensional anisotropic Heisenberg antiferromagnet. Inelastic scattering measurements show that incommensurate spin excitations survive at and above 50 K, where the elastic signal is negligible. Given that the stripe order is believed to be pinned by the low-temperature tetragonal (LTT) crystal structure, we have also investigated the transition near 70 K from the low-temperature orthorhombic (LTO) structure. We show that our x=0.12 crystal passes through an intervening less-orthorhombic phase, before reaching the LTT at ~40 K, whereas the x=0.15 crystal goes directly from LTO to LTT, with coexistence of the two phases over a range of ~7 K. Sharp Bragg peaks in the LTT phase of the x=0.15 crystal indicate a domain size of >~1000 Å , with no obvious evidence for LTO domains; hence, the coexistence of stripe order and superconductivity in this sample cannot be explained by a mixture of crystalline phases. Finally, we present scattering evidence for small LTT-like domains in the

  8. Understanding the heterogeneity in volume overload and fluid distribution in decompensated heart failure is key to optimal volume management: role for blood volume quantitation.

    Science.gov (United States)

    Miller, Wayne L; Mullan, Brian P

    2014-06-01

    This study sought to quantitate total blood volume (TBV) in patients hospitalized for decompensated chronic heart failure (DCHF) and to determine the extent of volume overload, and the magnitude and distribution of blood volume and body water changes following diuretic therapy. The accurate assessment and management of volume overload in patients with DCHF remains problematic. TBV was measured by a radiolabeled-albumin dilution technique with intravascular volume, pre-to-post-diuretic therapy, evaluated at hospital admission and at discharge. Change in body weight in relation to quantitated TBV was used to determine interstitial volume contribution to total fluid loss. Twenty-six patients were prospectively evaluated. Two patients had normal TBV at admission. Twenty-four patients were hypervolemic with TBV (7.4 ± 1.6 liters) increased by +39 ± 22% (range, +9.5% to +107%) above the expected normal volume. With diuresis, TBV decreased marginally (+30 ± 16%). Body weight declined by 6.9 ± 5.2 kg, and fluid intake/fluid output was a net negative 8.4 ± 5.2 liters. Interstitial compartment fluid loss was calculated at 6.2 ± 4.0 liters, accounting for 85 ± 15% of the total fluid reduction. TBV analysis demonstrated a wide range in the extent of intravascular overload. Dismissal measurements revealed marginally reduced intravascular volume post-diuretic therapy despite large reductions in body weight. Mobilization of interstitial fluid to the intravascular compartment with diuresis accounted for this disparity. Intravascular volume, however, remained increased at dismissal. The extent, composition, and distribution of volume overload are highly variable in DCHF, and this variability needs to be taken into account in the approach to individualized therapy. TBV quantitation, particularly serial measurements, can facilitate informed volume management with respect to a goal of treating to euvolemia. Copyright © 2014 American College of Cardiology Foundation. Published

  9. Variable volume combustor

    Energy Technology Data Exchange (ETDEWEB)

    Ostebee, Heath Michael; Ziminsky, Willy Steve; Johnson, Thomas Edward; Keener, Christopher Paul

    2017-01-17

    The present application provides a variable volume combustor for use with a gas turbine engine. The variable volume combustor may include a liner, a number of micro-mixer fuel nozzles positioned within the liner, and a linear actuator so as to maneuver the micro-mixer fuel nozzles axially along the liner.

  10. Improved images of crustal structures in the Bergslagen, central Sweden, through seismic reprocessing of BABEL lines 1, 6 and 7

    Science.gov (United States)

    Buntin, Sebastian; Malehmir, Alireza; Malinowski, Michał; Högdahl, Karin; Juhlin, Christopher; Buske, Stefan

    2017-04-01

    In a joint effort through the BABEL project, geoscientists from five countries acquired marine seismic data in the Baltic Sea with a total length of 2268 km in the year 1989. These consisted of near-vertical reflection and wide-angle refraction seismic data, providing insights into the subsurface down to the Moho and suggesting the existence of plate tectonics already during the Paleoproterozoic. The seismic data were acquired using a receiver group interval of 50 m and a total cable length of 3 km. In total, 60 groups of 64 hydrophones at 15 m depth were used. An airgun array consisting of six equal subarrays towed at 7.5 m depth was used to generate the seismic signal. The shot interval and the corresponding record lengths were different among the lines. A record length of 25 s and 75 m shot spacing for lines 1 and 7, respectively and 23 s and 62.5 m for line 6, respectively was used. The sampling rate was 4 ms for all three profiles. Lines 1, 6 and 7 are located at the boundary to the world-class and historical Bergslagen mineral district, and are being revisited in this study. Improved images can be used to refine previous interpretations, particularly at shallower depths (< 5 km). About 27 years after the acquisition, these data have been processed again in our study. Aside from the original processing steps, like spherical divergence correction, deconvolution and NMO corrections, additional processing steps such as DMO corrections or pre- and post-stack deconvolutions and coherency enhancements were applied. The reprocessing revealed reflections in the shallow part of the profiles, likely from major deformation (multi-phase) zones extending down to the lower crust, which were not present in the previous images. Also the images of the reflections in the deeper parts are remarkably improved. This also includes a few sub-Moho reflections. The three reprocessed profiles help constrain the nature of the northern boundary of Bergslagen and associated crustal

  11. Effects of nimodipine and fructose-1,6-diphosphate on cerebral damage in carbon monoxide poisoning mice

    Institute of Scientific and Technical Information of China (English)

    杨俊卿; 赵晓辉; 周岐新; 蒋青松

    2003-01-01

    Objective To study the dose- and time- dependent protective effects and the synergistic effects of nimodipine (NMDP) and fructose-1,6-diphosphate (FDP) against cerebral damage induced by acute carbon monoxide (CO) poisoning in mice.Methods Male mice were exposed to CO 170 mL/kg, i.p. After CO intraperitonealy exposure, mortality of mice, change in memory function estimated by passive avoidance test, the pathomorphologic observation of brain tissue slices, as well as changes of activities of monoamine oxidase (MAO)-B and Ca2+-Mg2+-ATPase in cerebral tissue were studied. In dose-dependent protective effect study, NMDP (10.6, 5.3, 2.7 mg/kg) and FDP (2.6, 1.3, 0.67 g/kg) was injected ip, respectively 15 min after CO exposure. To study the time-effect relationship of drugs, NMDP (5.3 mg/kg) and FDP (1.3 g/kg) were administered ip respectively 15 minutes, 45 minutes and 120 minutes after CO exposure. The combination of NMDP (2.7 mg/kg) and FDP (0.67 g/kg) was administered ip15 minutes, 45 min and 120 minutes after CO exposure to study the synergism of the two drugs.Results Either NMDP (10.6, 5.3 mg/kg) or FDP (2.6, 1.3 g/kg) administered ip within 15 minutes after CO exposure significantly decreased the impairment of memory function and mortality rate induced by CO, inhibited the decrease of Ca2+-Mg2+-ATPase activity, blunted the rising of MAO-B activity and prevented the delayed hippocampal neuronal death in poisoning mice. To our surprise, the combined use of NMDP (2.7 mg/kg) and FDP (0.67 g/kg) within 15 minutes after CO exposure had similar effects to that in NMDP (10.6, 5.3 mg/kg) and FDP (2.6, 1.3 g/kg).Conclusions These results suggest that the impairment of CO on brain can be attenuated if NMDP or FDP are administered sufficiently and quickly as soon as possible after CO exposure and there exists a synergism of FDP and NMDP against CO poisoning damage.

  12. NASA Ames Laminar Flow Supersonic Wind Tunnel (LFSWT) Tests of a 10 deg Cone at Mach 1.6

    Science.gov (United States)

    Wolf, Stephen W. D.; Laub, James A.

    1997-01-01

    This work is part of the ongoing qualification of the NASA Ames Laminar Flow Supersonic Wind Tunnel (LFSWT) as a low-disturbance (quiet) facility suitable for transition research. A 10 deg cone was tested over a range of unit Reynolds numbers (Re = 2.8 to 3.8 million per foot (9.2 to 12.5 million per meter)) and angles of incidence (O deg to 10 deg) at Mach 1.6. The location of boundary layer transition along the cone was measured primarily from surface temperature distributions, with oil flow interferometry and Schlieren flow visualization providing confirmation measurements. With the LFSWT in its normal quiet operating mode, no transition was detected on the cone in the test core, over the Reynolds number range tested at zero incidence and yaw. Increasing the pressure disturbance levels in the LFSWT test section by a factor of five caused transition onset on the cone within the test core, at zero incidence and yaw. When operating the LFSWT in its normal quiet mode, transition could only be detected in the test core when high angles of incidence (greater than 5 deg) for cones were set. Transition due to elevated pressure disturbances (Tollmien-Schlichting) and surface trips produced a skin temperature rise of order 4 F (2.2 C). Transition due to cross flows on the leeward side of the cone at incidence produced a smaller initial temperature rise of only order 2.5 F (1.4 C), which indicates a slower transition process. We can conclude that these cone tests add further proof that the LFSWT test core is normally low-disturbance (pressure fluctuations greater than 0.1%), as found by associated direct flow quality measurements discussed in this report. Furthermore, in a quiet test environment, the skin temperature rise is sensitive to the type of dominant instability causing transition. The testing of a cone in the LFSWT provides an excellent experiment for the development of advanced transition detection techniques.

  13. A comparative study of marine salmonid alphavirus subtypes 1-6 using an experimental cohabitation challenge model.

    Science.gov (United States)

    Graham, D A; Frost, P; McLaughlin, K; Rowley, H M; Gabestad, I; Gordon, A; McLoughlin, M F

    2011-04-01

    A comparative challenge study of six marine isolates representing subtypes 1-6 of salmonid alphavirus (salmon pancreas disease virus, Genus Alphavirus, Family Togaviridae) was conducted in Atlantic salmon in a fresh water cohabitation trial. Histopathological lesions typical of pancreas disease were observed with all subtypes, and virus was re-isolated from serum of cohabitant fish in each case. Using a virus neutralization (VN) test neutralizing salmonid alphavirus (SAV) subtype 1 strain F93-125, VN antibodies were detected in all challenge groups, consistent with serological cross-reactivity between these subtypes. Using real-time RT-PCR, SAV RNA was detected in heart tissue from 2 to 3 weeks post-challenge (wpc) in all cohabitant groups excluding controls. The results obtained suggested differences in the dynamics of infection between strains of SAV and potentially between subtypes. Results for SAV subtypes 1 and 3 suggested essentially synchronous infection of cohabitant fish. These two study groups also had the highest virus load in heart tissue as measured by quantitative RT-PCR and also had the most extensive histopathological changes. In contrast, results for SAV subtypes 2 and 6 strains were consistent with asynchronous infection in the cohabitant fish and were characterized by slow spread, low virus loads and mild histopathological changes. The SAV subtype 4 and 5 strains occupied an intermediate position in this regard. Despite the use of concentration procedures, it was not possible to detect SAV RNA in water samples from selected study tanks. However, testing of faeces from the SAV subtypes 1, 3 and 6 challenge groups found positive signals in each beginning at 1-3 wpc and remaining detectable for a further 2-3 weeks. Parallel testing of mucus samples found these became positive at 2-3 wpc and remained positive for a further 1-3 weeks. These results demonstrate for the first time that shedding and transmission of virus may occur by both these routes

  14. Volume Regulated Channels

    DEFF Research Database (Denmark)

    Klausen, Thomas Kjær

    of volume perturbations evolution have developed system of channels and transporters to tightly control volume homeostasis. In the past decades evidence has been mounting, that the importance of these volume regulated channels and transporters are not restricted to the defense of cellular volume...... but are also essential for a number of physiological processes such as proliferation, controlled cell death, migration and endocrinology. The thesis have been focusing on two Channels, namely the swelling activated Cl- channel (ICl, swell) and the transient receptor potential Vanilloid (TRPV4) channel. I: Cl......- serves a multitude of functions in the mammalian cell, regulating the membrane potential (Em), cell volume, protein activity and the driving force for facilitated transporters giving Cl- and Cl- channels a major potential of regulating cellular function. These functions include control of the cell cycle...

  15. Tööaja arvestamine ja lisatasude maksmine tükitöö puhul. Riigikohtu tsiviilkolleegiumi otsus asjas 3-2-1-6-08 / Merle Muda

    Index Scriptorium Estoniae

    Muda, Merle, 1972-

    2009-01-01

    Riigikohtu otsusest 3-2-1-6-08: OÜ Arliten (endine OÜ Anubis Expert) kassatsioonkaebus Tartu Ringkonnakohtu 03.10.2007. a otsusele Oliver Surva hagis OÜ Arliten (endine OÜ Anubis Expert) vastu töövaidluses

  16. The Constitutional Review Chamber of the Republic of Estonia : no. of the case 3-4-1-6-08 : date of decision 1 July 2008

    Index Scriptorium Estoniae

    2008-01-01

    Kohtulahendi 3-4-1-6-08 (Tallinna Ringkonnakohtu taotlus tunnistada põhiseadusega vastuolus olevaks lennundusseaduse § 71 lg 2 teine lause, millega on pandud ettevõtjale kontrolli teostamise kulude tasumise kohustus) tekst inglise keeles

  17. The Constitutional Review Chamber of the Republic of Estonia : no. of the case 3-4-1-6-08 : date of decision 1 July 2008

    Index Scriptorium Estoniae

    2008-01-01

    Kohtulahendi 3-4-1-6-08 (Tallinna Ringkonnakohtu taotlus tunnistada põhiseadusega vastuolus olevaks lennundusseaduse § 71 lg 2 teine lause, millega on pandud ettevõtjale kontrolli teostamise kulude tasumise kohustus) tekst inglise keeles

  18. Tööaja arvestamine ja lisatasude maksmine tükitöö puhul. Riigikohtu tsiviilkolleegiumi otsus asjas 3-2-1-6-08 / Merle Muda

    Index Scriptorium Estoniae

    Muda, Merle, 1972-

    2009-01-01

    Riigikohtu otsusest 3-2-1-6-08: OÜ Arliten (endine OÜ Anubis Expert) kassatsioonkaebus Tartu Ringkonnakohtu 03.10.2007. a otsusele Oliver Surva hagis OÜ Arliten (endine OÜ Anubis Expert) vastu töövaidluses

  19. Identification of arginine 331 as an important active site residue in the class II fructose-1,6-bisphosphate aldolase of Escherichia coli.

    OpenAIRE

    S Qamar; Marsh, K; Berry, A

    1996-01-01

    Treatment of the Class II fructose-1,6-bisphosphate aldolase of Escherichia coli with the arginine-specific alpha-dicarbonyl reagents, butanedione or phenylglyoxal, results in inactivation of the enzyme. The enzyme is protected from inactivation by the substrate, fructose 1,6-bisphosphate, or by inorganic phosphate. Modification with [7-14C] phenylglyoxal in the absence of substrate demonstrates that enzyme activity is abolished by the incorporation of approximately 2 moles of reagent per mol...

  20. Volume regulation in epithelia

    DEFF Research Database (Denmark)

    Larsen, Erik Hviid; Hoffmann, Else Kay

    2016-01-01

    function of iso-osmotic fluid transport that depends on Na+ recirculation. The causative relationship is discussed for a fluid-absorbing and a fluid-secreting epithelium of which the Na+ recirculation mechanisms have been identified. A large number of transporters and ion channels involved in cell volume...... regulation are cloned. The volume-regulated anion channel (VRAC) exhibiting specific electrophysiological characteristics seems exclusive to serve cell volume regulation. This is contrary to K+ channels as well as cotransporters and exchange mechanisms that may serve both transepithelial transport and cell...

  1. Towards the Amplituhedron Volume

    CERN Document Server

    Ferro, Livia; Orta, Andrea; Parisi, Matteo

    2015-01-01

    It has been recently conjectured that scattering amplitudes in planar N=4 super Yang-Mills are given by the volume of the (dual) amplituhedron. In this paper we show some interesting connections between the tree-level amplituhedron and a special class of differential equations. In particular we demonstrate how the amplituhedron volume for NMHV amplitudes is determined by these differential equations. The new formulation allows for a straightforward geometric description, without any reference to triangulations. Finally we discuss possible implications for volumes related to generic N^kMHV amplitudes.

  2. Unsteady flow volumes

    Energy Technology Data Exchange (ETDEWEB)

    Becker, B.G.; Lane, D.A.; Max, N.L.

    1995-03-01

    Flow volumes are extended for use in unsteady (time-dependent) flows. The resulting unsteady flow volumes are the 3 dimensional analog of streamlines. There are few examples where methods other than particle tracing have been used to visualize time varying flows. Since particle paths can become convoluted in time there are additional considerations to be made when extending any visualization technique to unsteady flows. We will present some solutions to the problems which occur in subdivision, rendering, and system design. We will apply the unsteady flow volumes to a variety of field types including moving multi-zoned curvilinear grids.

  3. Bronchoscopic lung volume reduction

    Directory of Open Access Journals (Sweden)

    M. I. Polkey

    2006-12-01

    Full Text Available Surgical lung volume reduction can improve exercise performance and forced expiratory volume in one second in patients with emphysema. However, the procedure is associated with a 5% mortality rate and a nonresponse rate of 25%. Accordingly, interest has focused on alternative ways of reducing lung volume. Two principle approaches are used: collapse of the diseased area using blockers placed endobronchially and the creation of extrapulmonary pathways. Preliminary data from the former approach suggest that it can be successful and that the magnitude of success is related to reduction in dynamic hyperinflation.

  4. The TGFBR1*6A allele is not associated with susceptibility to colorectal cancer in a Spanish population: a case-control study

    Directory of Open Access Journals (Sweden)

    Barberá Víctor-Manuel

    2009-06-01

    Full Text Available Abstract Background TGF-β receptor type I is a mediator of growth inhibitory signals. TGFBR1*6A (rs11466445 is a common polymorphic variant of the TGF-β receptor I gene and has been associated with tumour susceptibility. Nevertheless, the role of this polymorphism as a risk factor for colorectal cancer is controversial. The aim of this study was to assess the association between TGFBR1*6A and colorectal cancer, age, sex, tumour location and tumour stage in a Spanish population. Methods The case-control study involved 800 Spanish subjects: 400 sporadic colorectal cancer patients and 400 age-, sex-, and ethnic-matched controls. The odds ratio (OR and 95% confidence interval (95% CI for the TGFBR1*6A polymorphism were calculated using unconditional logistic regression adjusted for age and sex. Analysis of somatic mutations at the GCG repeat of TGFBR1 exon 1 and germline allele-specific expression were also conducted to obtain further information on the contribution of the TGFBR1*6A allele to CRC susceptibility. Results There was no statistically significant association between the TGFBR1*6A allele and CRC (p > 0.05. The OR was 1.147 (95% CI: 0.799–1.647 for carriers of the TGFBR1*6A allele and 0.878 (95% CI: 0.306–2.520 for homozygous TGFBR1*6A individuals compared with the reference. The frequency of the polymorphism was not affected by age, sex or tumour stage. The TGFBR1*6A allele was more prevalent among colon tumour patients than among rectal tumour patients. Tumour somatic mutations were found in only two of 69 cases (2.9%. Both cases involved a GCG deletion that changed genotype 9A/9A in normal DNA to genotype 9A/8A. Interestingly, these two tumours were positive for microsatellite instability, suggesting that these mutations originated because of a deficient DNA mismatch repair system. Allele-specific expression of the 9A allele was detected in seven of the 14 heterozygous 9A/6A tumour cases. This could have been caused by linkage

  5. Free volume under shear

    Science.gov (United States)

    Maiti, Moumita; Vinutha, H. A.; Sastry, Srikanth; Heussinger, Claus

    2015-10-01

    Using an athermal quasistatic simulation protocol, we study the distribution of free volumes in sheared hard-particle packings close to, but below, the random-close packing threshold. We show that under shear, and independent of volume fraction, the free volumes develop features similar to close-packed systems — particles self-organize in a manner as to mimick the isotropically jammed state. We compare athermally sheared packings with thermalized packings and show that thermalization leads to an erasure of these structural features. The temporal evolution in particular the opening-up and the closing of free-volume patches is associated with the single-particle dynamics, showing a crossover from ballistic to diffusive behavior.

  6. Integers annual volume 2013

    CERN Document Server

    Landman, Bruce

    2014-01-01

    ""Integers"" is a refereed online journal devoted to research in the area of combinatorial number theory. It publishes original research articles in combinatorics and number theory. This work presents all papers of the 2013 volume in book form.

  7. Renormalized Volumes with Boundary

    CERN Document Server

    Gover, A Rod

    2016-01-01

    We develop a general regulated volume expansion for the volume of a manifold with boundary whose measure is suitably singular along a separating hypersurface. The expansion is shown to have a regulator independent anomaly term and a renormalized volume term given by the primitive of an associated anomaly operator. These results apply to a wide range of structures. We detail applications in the setting of measures derived from a conformally singular metric. In particular, we show that the anomaly generates invariant (Q-curvature, transgression)-type pairs for hypersurfaces with boundary. For the special case of anomalies coming from the volume enclosed by a minimal hypersurface ending on the boundary of a Poincare--Einstein structure, this result recovers Branson's Q-curvature and corresponding transgression. When the singular metric solves a boundary version of the constant scalar curvature Yamabe problem, the anomaly gives generalized Willmore energy functionals for hypersurfaces with boundary. Our approach ...

  8. Environmental chemistry: Volume A

    Energy Technology Data Exchange (ETDEWEB)

    Yen, T.F.

    1999-08-01

    This is an extensive introduction to environmental chemistry for engineering and chemical professionals. The contents of Volume A include a brief review of basic chemistry prior to coverage of litho, atmo, hydro, pedo, and biospheres.

  9. A facile and effective synthesis of alpha-(1-->6)-linked mannose di-, tri-, tetra-, hexa-, octa-, and dodecasaccharides, and beta-(1-->6)-linked glucose di-, tri-, tetra-, hexa-, and octasaccharides using sugar trichloroacetimidates as the donors and unprotected or partially protected glycosides as the acceptors.

    Science.gov (United States)

    Zhu, Y; Kong, F

    2001-05-08

    Reaction of 2,3,4,6-tetra-O-acetyl-alpha-D-mannopyranosyl trichloroimidate with allyl alpha-D-mannopyranoside in the presence of TMSOTf selectively gave allyl 2,3,4,6-tetra-O-acetyl-alpha-D-mannopyranosyl-(1-->6)-alpha-D-mannopyranoside through an orthoester intermediate. Benzoylation of 3, followed by deallylation, and then trichloroimidation afforded the disaccharide donor 2,3,4,6-tetra-O-acetyl-alpha-D-mannopyranosyl-(1-->6)-2,3,4-tri-O-benzoyl-alpha-D-mannopyranosyl trichloroimidate, while benzoylation of 3 followed by selective removal of acetyl groups yielded the disaccharide acceptor allyl alpha-D-mannopyranosyl-(1-->6)-2,3,4-tri-O-benzoyl-alpha-D-mannopyranoside. Coupling of 5 with 6 gave the tetrasaccharide allyl 2,3,4,6-tetra-O-acetyl-alpha-D-mannopyranosyl-(1-->6)-2,3,4-tri-O-benzoyl-alpha-D-mannopyranosyl-(1-->6)-alpha-D-mannopyranosyl-(1-->6)-2,3,4-tri-O-benzoyl-alpha-D-mannopyranoside, which were converted into the tetrasaccharide donor 2,3,4,6-tetra-O-acetyl-alpha-D-mannopyranosyl-(1-->6)-2,3,4-tri-O-benzoyl-alpha-D-mannopyranosyl-(1-->6)-2,3,4-tri-O-benzoyl-alpha-D-mannopyranosyl-(1-->6)-2,3,4-tri-O-benzoyl-alpha-D-mannopyranosyl trichloroimdate and the tetrasaccharide acceptor allyl alpha-D-mannopyranosyl-(1-->6)-2,3,4-tri-O-benzoyl-alpha-D-mannopyranosyl-(1-->6)-2,3,4-tri-O-benzoyl-alpha-D-mannopyranosyl-(1-->6)-2,3,4-tri-O-benzoyl-alpha-D-mannopyranoside, respectively, by the same strategies as used for conversion of 3 into 5 and 6. Condensation of 5 with 13 gave the hexasaccharide 14, while condensation of 12 with 13 gave the octasaccharide 17. Dodecasaccharide 21 was obtained by the coupling of 12 with the octasaccharide acceptor 20. Similar strategies were used for the syntheses of beta-(1-->6)-linked glucose di-, tri-, tetra-, hexa-, and octamers. Deprotection of the oligosaccharides in ammonia-saturated methanol yielded the free alpha-(1-->6)-linked mannosyl and beta-(1-->6)-linked glucosyl oligomers.

  10. Generalized Partial Volume

    DEFF Research Database (Denmark)

    Darkner, Sune; Sporring, Jon

    2011-01-01

    Mutual Information (MI) and normalized mutual information (NMI) are popular choices as similarity measure for multimodal image registration. Presently, one of two approaches is often used for estimating these measures: The Parzen Window (PW) and the Generalized Partial Volume (GPV). Their theoret......Mutual Information (MI) and normalized mutual information (NMI) are popular choices as similarity measure for multimodal image registration. Presently, one of two approaches is often used for estimating these measures: The Parzen Window (PW) and the Generalized Partial Volume (GPV...

  11. Reachable volume RRT

    KAUST Repository

    McMahon, Troy

    2015-05-01

    © 2015 IEEE. Reachable volumes are a new technique that allows one to efficiently restrict sampling to feasible/reachable regions of the planning space even for high degree of freedom and highly constrained problems. However, they have so far only been applied to graph-based sampling-based planners. In this paper we develop the methodology to apply reachable volumes to tree-based planners such as Rapidly-Exploring Random Trees (RRTs). In particular, we propose a reachable volume RRT called RVRRT that can solve high degree of freedom problems and problems with constraints. To do so, we develop a reachable volume stepping function, a reachable volume expand function, and a distance metric based on these operations. We also present a reachable volume local planner to ensure that local paths satisfy constraints for methods such as PRMs. We show experimentally that RVRRTs can solve constrained problems with as many as 64 degrees of freedom and unconstrained problems with as many as 134 degrees of freedom. RVRRTs can solve problems more efficiently than existing methods, requiring fewer nodes and collision detection calls. We also show that it is capable of solving difficult problems that existing methods cannot.

  12. Biophysical characterisation of the persistent sodium current of the Nav1.6 neuronal sodium channel: a single-channel analysis.

    Science.gov (United States)

    Chatelier, Aurélien; Zhao, Juan; Bois, Patrick; Chahine, Mohamed

    2010-06-01

    Na(v)1.6 is the major voltage-gated sodium channel at nodes of Ranvier. This channel has been shown to produce a robust persistent inward current in whole-cell experiments. Na(v)1.6 plays an important role in axonal conduction and may significantly contribute to the pathophysiology of the injured nervous system through this persistent current. However, the underlying molecular mechanisms and regulation of the persistent current are not well understood. Using the whole-cell configuration of the patch-clamp technique, we investigated the Na(v)1.6 transient and persistent currents in HEK-293. Previous studies have shown that the persistent current depended on the content of the patch electrode. Therefore, we characterised the single-channel properties of the persistent current with an intact intracellular medium using the cell-attached configuration of the patch-clamp technique. In HEK-293 cells, the Na(v)1.6 persistent current recorded in the whole-cell configuration was 3-5% of the peak transient current. In single-channel recording, the ratio between peak and persistent open probability confirmed the magnitude of the persistent current observed in the whole-cell configuration. The cell-attached configuration revealed that the molecular mechanism of the whole-cell persistent current is a consequence of single Na(v)1.6 channels reopening.

  13. β-1,3/1,6-Glucan alleviated intestinal mucosal barrier impairment of broiler chickens challenged with Salmonella enterica serovar Typhimurium.

    Science.gov (United States)

    Shao, Yujing; Guo, Yuming; Wang, Zhong

    2013-07-01

    This study investigated the protective effect of β-1,3/1,6-glucan on gut morphology, intestinal epithelial tight junctions, and bacterial translocation of broiler chickens challenged with Salmonella enterica serovar Typhimurium. Ninety Salmonella-free Arbor Acre male broiler chickens were randomly divided into 3 groups: negative control group (NC), Salmonella Typhimurium-infected positive group (PC), and the Salmonella Typhimurium-infected group with dietary 100 mg/kg of β-1,3/1,6-glucan supplementation (T) to determine the effect of β-1,3/1,6-glucan on intestinal barrier function. Salmonella Typhimurium challenge alone significantly decreased villus height (P cells (P cells at 14 dpi (P cells (P cells (P Salmonella Typhimurium in comparison with Salmonella Typhimurium challenge alone. β-1,3/1,6-Glucan addition had significant inhibitory effects (P Salmonella colonization levels and liver Salmonella invasion of the Salmonella Typhimurium-infected birds compared with the PC group. Intestinal tight junction proteins claudin-1, claudin-4, and occludin mRNA expression in the jejunum at 14 dpi was significantly decreased by Salmonella Typhimurium challenge alone (P Salmonella Typhimurium-infected birds in comparison with the PC group. Our results indicate that dietary β-1,3/1,6-glucan can alleviate intestinal mucosal barrier impairment in broiler chickens challenged with Salmonella Typhimurium.

  14. Effect of Ba Filling Fraction on Thermoelectric Properties of p-type BayFe1.6Co2.4Sb12

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Effects of Ba filling fraction on the thermoelectric properties of p-type filled skutterudite compounds BayFe1.6Co2.4Sb12(y=0-0.63) were investigated.BayFe1.6Co2.4Sb12 showed p-type conduction.The hall coefficient (RH) and seebeck coefficient(α) increased with increasing Ba filling fraction.But hole concentration (p) and electrical conductivity (σ) decreased.While Ba filling fraction was about 0.4,the lattice thermal conductivity (κL) reached the minimum value.The results show that effect of Ba rattling on phonon scattering is the strongest as Sb-icosahedron partial voids are filled by Ba.A maximum dimensionless thermoelectric figure of merit (ZT) value of 0.7 was obtained for Ba0.38Fe1.6Co2.4 Sb12 at 750K.

  15. The permeation of dynorphin A 1-6 across the blood brain barrier and its effect on bovine brain microvessel endothelial cell monolayer permeability.

    Science.gov (United States)

    Sloan, Courtney D Kuhnline; Audus, Kenneth L; Aldrich, Jane V; Lunte, Susan M

    2012-12-01

    Dynorphin A 1-17 (Dyn A 1-17) is an endogenous neuropeptide known to act at the kappa opioid receptor; it has been implicated in a number of neurological disorders, including neuropathic pain, stress, depression, and Alzheimer's and Parkinson's diseases. The investigation of Dyn A 1-17 metabolism at the blood-brain barrier (BBB) is important since the metabolites exhibit unique biological functions compared to the parent compound. In this work, Dyn A 1-6 is identified as a metabolite of Dyn A 1-17 in the presence of bovine brain microvessel endhothelial cells (BBMECs), using LC-MS/MS. The transport of Dyn A 1-6 at the BBB was examined using this in vitro cell culture model of the BBB. Furthermore, the permeation of the BBB by the low molecular weight permeability marker fluorescein was characterized in the presence and absences of Dyn A 1-6.

  16. Sistematies verwronge kommunikasie in Lukas 14:1-6: Die dialekties-kritiese teorie van Jürgen Habermas krities bespreek

    OpenAIRE

    1994-01-01

    Systematically distorted communication in Luke 14:1-6: A critical assessment of the dialectical-critical theory of Jürgen Habermas In this article the epistemology and essence of the practical interest of Jürgen Habermas’s dialectical-critical theory is discussed, focusing on his concept of systematically distorted communication. The article aims at describing the theory of communicative competence or universal pragmatics by means of an exegetical study of Luke 14:1-6. The appropriation of Ha...

  17. Inhibition of chloroplastic fructose 1,6-bisphosphatase in tomato fruits leads to decreased fruit size, but only small changes in carbohydrate metabolism

    DEFF Research Database (Denmark)

    Obiadalla-Ali, H.; Fernie, A.R.; Lytovchenko, A.;

    2004-01-01

    A potato (Solanum tuberosum L. ) cDNA coding for the chloroplastic isoform of fructose 1,6-bisphosphatase (cp-FBPase) was utilized to repress its activity in tomatoes (Lycopersicon esculentum Mill.) using antisense techniques. The patatin B33 promoter was used to ensure fruit specificity of the a......A potato (Solanum tuberosum L. ) cDNA coding for the chloroplastic isoform of fructose 1,6-bisphosphatase (cp-FBPase) was utilized to repress its activity in tomatoes (Lycopersicon esculentum Mill.) using antisense techniques. The patatin B33 promoter was used to ensure fruit specificity...

  18. Volumes of chain links

    CERN Document Server

    Kaiser, James; Rollins, Clint

    2011-01-01

    Agol has conjectured that minimally twisted n-chain links are the smallest volume hyperbolic manifolds with n cusps, for n at most 10. In his thesis, Venzke mentions that these cannot be smallest volume for n at least 11, but does not provide a proof. In this paper, we give a proof of Venzke's statement. The proof for n at least 60 is completely rigorous. The proof for n between 11 and 59 uses a computer calculation, and can be made rigorous for manifolds of small enough complexity, using methods of Moser and Milley. Finally, we prove that the n-chain link with 2m or 2m+1 half-twists cannot be the minimal volume hyperbolic manifold with n cusps, provided n is at least 60 or |m| is at least 8, and we give computational data indicating this remains true for smaller n and |m|.

  19. HARNESSING BIG DATA VOLUMES

    Directory of Open Access Journals (Sweden)

    Bogdan DINU

    2014-04-01

    Full Text Available Big Data can revolutionize humanity. Hidden within the huge amounts and variety of the data we are creating we may find information, facts, social insights and benchmarks that were once virtually impossible to find or were simply inexistent. Large volumes of data allow organizations to tap in real time the full potential of all the internal or external information they possess. Big data calls for quick decisions and innovative ways to assist customers and the society as a whole. Big data platforms and product portfolio will help customers harness to the full the value of big data volumes. This paper deals with technical and technological issues related to handling big data volumes in the Big Data environment.

  20. Topological Active Volumes

    Directory of Open Access Journals (Sweden)

    Barreira N

    2005-01-01

    Full Text Available The topological active volumes (TAVs model is a general model for 3D image segmentation. It is based on deformable models and integrates features of region-based and boundary-based segmentation techniques. Besides segmentation, it can also be used for surface reconstruction and topological analysis of the inside of detected objects. The TAV structure is flexible and allows topological changes in order to improve the adjustment to object's local characteristics, find several objects in the scene, and identify and delimit holes in detected structures. This paper describes the main features of the TAV model and shows its ability to segment volumes in an automated manner.

  1. Aperiodic Volume Optics

    Science.gov (United States)

    Gerke, Tim D.

    Presented in this thesis is an investigation into aperiodic volume optical devices. The three main topics of research and discussion are the aperiodic volume optical devices that we call computer-generated volume holograms (CGVH), defects within periodic 3D photonic crystals, and non-periodic, but ordered 3D quasicrystals. The first of these devices, CGVHs, are designed and investigated numerically and experimentally. We study the performance of multi-layered amplitude computer-generated volume holograms in terms of efficiency and angular/frequency selectivity. Simulation results show that such aperiodic devices can increase diffraction efficiency relative to periodic amplitude volume holograms while maintaining angular and wavelength selectivity. CGVHs are also designed as voxelated volumes using a new projection optimization algorithm. They are investigated using a volumetric diffraction simulation and a standard 3D beam propagation technique as well as experimentally. Both simulation and experiment verify that the structures function according to their design. These represent the first diffractive structures that have the capacity for generating arbitrary transmission and reflection wave fronts and that provide the ability for multiplexing arbitrary functionality given different illumination conditions. Also investigated and discussed in this thesis are 3D photonic crystals and quasicrystals. We demonstrate that these devices can be fabricated using a femtosecond laser direct writing system that is particularly appropriate for fabrication of such arbitrary 3D structures. We also show that these devices can provide 3D partial bandgaps which could become complete bandgaps if fabricated using high index materials or by coating lower index materials with high index metals. Our fabrication method is particularly suited to the fabrication of engineered defects within the periodic or quasi-periodic systems. We demonstrate the potential for fabricating defects within

  2. The volume of a soliton

    Energy Technology Data Exchange (ETDEWEB)

    Adam, C., E-mail: adam@fpaxp1.usc.es [Departamento de Física de Partículas, Universidad de Santiago de Compostela and Instituto Galego de Física de Altas Enerxias (IGFAE), E-15782 Santiago de Compostela (Spain); Haberichter, M. [School of Mathematics, Statistics and Actuarial Science, University of Kent, Canterbury, CT2 7NF (United Kingdom); Wereszczynski, A. [Institute of Physics, Jagiellonian University, Lojasiewicza 11, Kraków (Poland)

    2016-03-10

    There exists, in general, no unique definition of the size (volume, area, etc., depending on dimension) of a soliton. Here we demonstrate that the geometric volume (area etc.) of a soliton is singled out in the sense that it exactly coincides with the thermodynamical or continuum-mechanical volume. In addition, this volume may be defined uniquely for rather arbitrary solitons in arbitrary dimensions.

  3. A one-pot synthesis of 1,6,9,13-tetraoxadispiro(4.2.4.2)tetradecane by hydrodeoxygenation of xylose using a palladium catalyst

    Science.gov (United States)

    In an effort to expand the number of biobased chemicals available from sugars, xylose has been converted to 1,6,9,13-tetraoxadispiro(4.2.4.2)tetradecane in a one-pot reaction using palladium supported on silica-alumina as the catalyst. The title compound is produced in 35-40% yield under 7 MPa H2 pr...

  4. An Interleaving Track & Hold with 7.6 ENOB @ 1.6 GS/s in 0.12 µm CMOS

    NARCIS (Netherlands)

    Louwsma, S.M.; van Tuijl, Adrianus Johannes Maria; Vertregt, Maarten; Scholtens, Peter C.S.; Nauta, Bram

    2004-01-01

    A 1.6 GS/s Track and Hold circuit that produces 16 interleaving, 100 MS/s voltage buffered output signals is presented. The achieved SFDR for a 950 MHz full scale input signal is 50 dB. Phase alignment is 0.4 ps RMS and aperture uncertainty is 1 ps RMS. The chip includes two Analog to Digital Conver

  5. PtCl2-catalyzed tandem enyne cyclization/1,2 ester migration reaction controlled by substituent effects of all-carbon 1,6-enynyl esters.

    Science.gov (United States)

    Huo, Xing; Zhao, Changgui; Zhao, Gaoyuan; Tang, Shouchu; Li, Huilin; Xie, Xingang; She, Xuegong

    2013-05-01

    On the move: A novel PtCl2-catalyzed tandem 1,6-enyne cyclization/1,2-acyloxy migration reaction was developed, which was shown to be controlled by substitution effects. Using this method, a series of substituted enol esters containing the cyclopentenyl motif were prepared in moderate to high yields.

  6. 47 CFR 25.143 - Licensing provisions for the 1.6/2.4 GHz mobile-satellite service and 2 GHz mobile-satellite...

    Science.gov (United States)

    2010-10-01

    ...-satellite service and 2 GHz mobile-satellite service. 25.143 Section 25.143 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) COMMON CARRIER SERVICES SATELLITE COMMUNICATIONS Applications and Licenses Space Stations § 25.143 Licensing provisions for the 1.6/2.4 GHz mobile-satellite service and 2...

  7. Synthesis, Structural Characterization, and Antitumor Activity of a Ca(II Coordination Polymer Based on 1,6-Naphthalenedisulfonate and 4,4′-Bipyridyl

    Directory of Open Access Journals (Sweden)

    Xishi Tai

    2013-08-01

    Full Text Available A novel Ca(II coordination polymer, [CaL(4,4′-bipyridyl(H2O4]n (L = 1,6-naphthalenedisulfonate, was synthesized by reaction of calcium perchlorate with 1,6-naphthalenedisulfonic acid disodium salt and 4,4′-bipyridyl in CH3CH2OH/H2O. It was characterized by elemental analysis, IR, molar conductivity and thermogravimetric analysis. X-ray crystallography reveals that the Ca(II coordination polymer belongs to the orthorhombic system, with space group P212121. The geometry of the Ca(II ion is a distorted CaNO6 pengonal bipyramid, arising from its coordination by four water molecules, one nitrogen atom of 4,4′-bipyridyl molecule, and two oxygen atoms from two L ligands. The complex molecules form a helical chain by self-assembly. The antitumor activity of 1,6-naphthalenedisulfonic acid disodium salt and the Ca(II coordination polymer against human hepatoma smmc-7721 cell line and human lung adenocarcinoma A549 cell line reveals that the Ca(II coordination polymer inhibits cell growth of human lung adenocarcinoma A549 cell line with IC50 value of 27 μg/mL, and is more resistive to human lung adenocarcinoma A549 cell line as compared to 1,6-naphthalenedisulfonic acid disodium salt.

  8. Production and characterization of high-titer serum-free cell culture grown hepatitis C virus particles of genotype 1-6

    DEFF Research Database (Denmark)

    Mathiesen, Christian K; Jensen, Tanja B; Prentoe, Jannick

    2014-01-01

    Recently, cell culture systems producing hepatitis C virus particles (HCVcc) were developed. Establishment of serum-free culture conditions is expected to facilitate development of a whole-virus inactivated HCV vaccine. We describe generation of genotype 1-6 serum-free HCVcc (sf-HCVcc) from Huh7...

  9. Challenge pools of hepatitis C virus genotypes 1-6 prototype strains: replication fitness and pathogenicity in chimpanzees and human liver-chimeric mouse models

    DEFF Research Database (Denmark)

    Bukh, Jens; Meuleman, Philip; Tellier, Raymond

    2010-01-01

    Chimpanzees represent the only animal model for studies of the natural history of hepatitis C virus (HCV). To generate virus stocks of important HCV variants, we infected chimpanzees with HCV strains of genotypes 1-6 and determined the infectivity titer of acute-phase plasma pools in additional a...

  10. An ATP Binding Cassette Transporter Mediates the Uptake of α-(1,6)-Linked Dietary Oligosaccharides in Bifidobacterium and Correlates with Competitive Growth on These Substrates

    DEFF Research Database (Denmark)

    Hansen, Morten Ejby; Fredslund, Folmer; Andersen, Joakim Mark

    2016-01-01

    and lactobacilli in the human gut. Here we show that the solute binding protein (BlG16BP) associated with an ATP binding cassette (ABC) transporter from the probiotic Bifidobacterium animalis subsp. lactis Bl-04 binds -(1,6)-linked glucosides and galactosides of varying size, linkage, and monosaccharide...

  11. A novel mechanism for the promotion of quercetin glycoside absorption by megalo α-1,6-glucosaccharide in the rat small intestine.

    Science.gov (United States)

    Shinoki, Aki; Lang, Weeranuch; Thawornkuno, Charin; Kang, Hee-Kwon; Kumagai, Yuya; Okuyama, Masayuki; Mori, Haruhide; Kimura, Atsuo; Ishizuka, Satoshi; Hara, Hiroshi

    2013-01-15

    The presence of an α-1,6-glucosaccharide enhances absorption of water-soluble quercetin glycosides, a mixture of quercetin-3-O-β-d-glucoside (Q3G, 31.8%), mono (23.3%), di (20.3%) and more d-glucose adducts with α-1,4-linkage to a d-glucose moiety of Q3G, in a ligated small intestinal loop of anesthetized rats. We prepared α-1,6-glucosaccharides with different degrees of polymerization (DP) enzymatically and separated them into a megalo-isomaltosaccharide-containing fraction (M-IM, average DP=11.0) and an oligo-isomaltosaccharide-containing fraction (O-IM, average DP=3.6). Luminal injection of either saccharide fraction promoted the absorption of total quercetin-derivatives from the small intestinal segment and this effect was greater for M-IM than O-IM addition. M-IM also increased Q3G, but not the quercetin aglycone, concentration in the water-phase of the luminal contents more strongly than O-IM. The enhancement of Q3G solubilization in the luminal contents may be responsible for the increases in the quercetin glucoside absorption promoted by α-1,6-glucosaccharides, especially that by M-IM. These results suggest that the ingestion of α-1,6-glucosaccharides promotes Q3G bioavailability.

  12. An ATP Binding Cassette Transporter Mediates the Uptake of alpha-(1,6)-Linked Dietary Oligosaccharides in Bifidobacterium and Correlates with Competitive Growth on These Substrates

    NARCIS (Netherlands)

    Ejby, Morten; Fredslund, Folmer; Andersen, Joakim Mark; Zagar, Andreja Vujicic; Henriksen, Jonas Rosager; Andersen, Thomas Lars; Svensson, Birte; Slotboom, Dirk Jan; Abou Hachem, Maher

    2016-01-01

    The molecular details and impact of oligosaccharide uptake by distinct human gut microbiota (HGM) are currently not well understood. Non-digestible dietary galacto- and gluco--(1,6)-oligosaccharides from legumes and starch, respectively, are preferentially fermented by mainly bifidobacteria and

  13. Expression of sodium channel α subunits 1.1, 1.2 and 1.6 in rat hippocampus after kainic acid-induced epilepsy

    NARCIS (Netherlands)

    Qiao, X.; Werkman, T.R.; Gorter, J.A.; Wadman, W.J.; van Vliet, E.A.

    2013-01-01

    Voltage-gated Na(+) channels control neuronal excitability and are the primary target for the majority of anti-epileptic drugs. This study investigates the (sub)cellular expression patterns of three important brain-associated Na(+) channel α subunits: NaV1.1, NaV1.2 and NaV1.6 during epileptogenesis

  14. Replacement of glutamic acid 29 with glutamine leads to a loss of cooperativity for AMP with porcine fructose-1,6-bisphosphatase.

    Science.gov (United States)

    Chen, M; Chen, L; Fromm, H J

    1994-02-25

    Mutations in the AMP binding site of porcine fructose-1,6-bisphosphatase were carried out by site-specific mutagenesis based on the crystal structure of the enzyme (Ke, H., Zhang, Y., and Lipscomb, W.L. (1990) Proc. Natl. Acad. Sci. U.S.A. 87, 5243-5247). The mutant and wild-type enzymes were characterized by SDS-polyacrylamide gel electrophoresis, circular dichroism spectrometry, and initial rate kinetics. One of the mutant forms of fructose-1,6-bisphosphatase, Glu-29-->Gln, is ligated to the phosphoryl moiety of AMP, a potent inhibitor of the reaction, whereas the other mutant, Thr-31-->Val, is associated with the purine base of AMP. No discernible alteration in structure as measured by circular dichroism spectrometry was noted for the mutants relative to the wild-type enzyme. As expected, major changes in kinetic parameters between the mutants and the wild-type enzyme were associated with inhibition by AMP. AMP, a competitive inhibitor with respect to Mg2+ in the fructose-1,6-bisphosphatase reaction, exhibits cooperativity in the case of the wild-type and the mutant Thr-31-->Val enzymes with a Hill coefficient of 2.0. On the other hand, cooperativity is completely lost in the case of Glu-29-->Gln fructose-1,6-bisphosphatase.

  15. Identification of Amino Acid Residues in Fibroblast Growth Factor 14 (FGF14) Required for Structure-Function Interactions with Voltage-gated Sodium Channel Nav1.6.

    Science.gov (United States)

    Ali, Syed R; Singh, Aditya K; Laezza, Fernanda

    2016-05-20

    The voltage-gated Na(+) (Nav) channel provides the basis for electrical excitability in the brain. This channel is regulated by a number of accessory proteins including fibroblast growth factor 14 (FGF14), a member of the intracellular FGF family. In addition to forming homodimers, FGF14 binds directly to the Nav1.6 channel C-tail, regulating channel gating and expression, properties that are required for intrinsic excitability in neurons. Seeking amino acid residues with unique roles at the protein-protein interaction interface (PPI) of FGF14·Nav1.6, we engineered model-guided mutations of FGF14 and validated their impact on the FGF14·Nav1.6 complex and the FGF14:FGF14 dimer formation using a luciferase assay. Divergence was found in the β-9 sheet of FGF14 where an alanine (Ala) mutation of Val-160 impaired binding to Nav1.6 but had no effect on FGF14:FGF14 dimer formation. Additional analysis revealed also a key role of residues Lys-74/Ile-76 at the N-terminal of FGF14 in the FGF14·Nav1.6 complex and FGF14:FGF14 dimer formation. Using whole-cell patch clamp electrophysiology, we demonstrated that either the FGF14(V160A) or the FGF14(K74A/I76A) mutation was sufficient to abolish the FGF14-dependent regulation of peak transient Na(+) currents and the voltage-dependent activation and steady-state inactivation of Nav1.6; but only V160A with a concomitant alanine mutation at Tyr-158 could impede FGF14-dependent modulation of the channel fast inactivation. Intrinsic fluorescence spectroscopy of purified proteins confirmed a stronger binding reduction of FGF14(V160A) to the Nav1.6 C-tail compared with FGF14(K74A/I76A) Altogether these studies indicate that the β-9 sheet and the N terminus of FGF14 are well positioned targets for drug development of PPI-based allosteric modulators of Nav channels.

  16. Mnn10 Maintains Pathogenicity in Candida albicans by Extending α-1,6-Mannose Backbone to Evade Host Dectin-1 Mediated Antifungal Immunity.

    Directory of Open Access Journals (Sweden)

    Shi Qun Zhang

    2016-05-01

    Full Text Available The cell wall is a dynamic structure that is important for the pathogenicity of Candida albicans. Mannan, which is located in the outermost layer of the cell wall, has been shown to contribute to the pathogenesis of C. albicans, however, the molecular mechanism by which this occurs remains unclear. Here we identified a novel α-1,6-mannosyltransferase encoded by MNN10 in C. albicans. We found that Mnn10 is required for cell wall α-1,6-mannose backbone biosynthesis and polysaccharides organization. Deletion of MNN10 resulted in significant attenuation of the pathogenesis of C. albicans in a murine systemic candidiasis model. Inhibition of α-1,6-mannose backbone extension did not, however, impact the invasive ability of C. albicans in vitro. Notably, mnn10 mutant restored the invasive capacity in athymic nude mice, which further supports the notion of an enhanced host antifungal defense related to this backbone change. Mnn10 mutant induced enhanced Th1 and Th17 cell mediated antifungal immunity, and resulted in enhanced recruitment of neutrophils and monocytes for pathogen clearance in vivo. We also demonstrated that MNN10 could unmask the surface β-(1,3-glucan, a crucial pathogen-associated molecular pattern (PAMP of C. albicans recognized by host Dectin-1. Our results demonstrate that mnn10 mutant could stimulate an enhanced Dectin-1 dependent immune response of macrophages in vitro, including the activation of nuclear factor-κB, mitogen-activated protein kinase pathways, and secretion of specific cytokines such as TNF-α, IL-6, IL-1β and IL-12p40. In summary, our study indicated that α-1,6-mannose backbone is critical for the pathogenesis of C. albicans via shielding β-glucan from recognition by host Dectin-1 mediated immune recognition. Moreover, our work suggests that inhibition of α-1,6-mannose extension by Mnn10 may represent a novel modality to reduce the pathogenicity of C. albicans.

  17. Thermoelectric Properties of Alumina-Doped Bi0.4Sb1.6Te3 Nanocomposites Prepared through Mechanical Alloying and Vacuum Hot Pressing

    Directory of Open Access Journals (Sweden)

    Chung-Kwei Lin

    2015-11-01

    Full Text Available In this study, γ-Al2O3 particles were dispersed in p-type Bi0.4Sb1.6Te3 through mechanical alloying to form γ-Al2O3/Bi0.4Sb1.6Te3 composite powders. The composite powders were consolidated using vacuum hot pressing to produce nano- and microstructured composites. Thermoelectric (TE measurements indicated that adding an optimal amount of γ-Al2O3 nanoparticles improves the TE performance of the fabricated composites. High TE performances with figure of merit (ZT values as high as 1.22 and 1.21 were achieved at 373 and 398 K for samples containing 1 and 3 wt % γ-Al2O3 nanoparticles, respectively. These ZT values are higher than those of monolithic Bi0.4Sb1.6Te3 samples. The ZT values of the fabricated samples at 298–423 K are 1.0–1.22; these ZT characteristics make γ-Al2O3/Bi0.4Sb1.6Te3 composites suitable for power generation applications because no other material with a similarly high ZT value has been reported at this temperature range. The achieved high ZT value may be attributable to the unique nano- and microstructures in which γ-Al2O3 nanoparticles are dispersed among the grain boundary or in the matrix grain, as revealed by high-resolution transmission electron microscopy. The dispersed γ-Al2O3 nanoparticles thus increase phonon scattering sites and reduce thermal conductivity. The results indicated that the nano- and microstructured γ-Al2O3/Bi0.4Sb1.6Te3 alloy can serve as a high-performance material for application in TE devices.

  18. Design for volume reduction

    NARCIS (Netherlands)

    Wever, R.; Boks, C.; Stevels, A.

    2007-01-01

    Traditionally packaging design-for-sustainability (DfS) strongly focuses on resource conservation and material recycling. The type and amount of materials used has been the driver in design. For consumer electronics (CE) products this weight-based approach is too limited; a volume-based approach is

  19. Introduction to the Volume.

    Science.gov (United States)

    Emihovich, Catherine; Schroder, Barbara; Panofsky, Carolyn P.

    1999-01-01

    Introduces a volume that examines the issue of critical thinking and whether or not it is culturally specific, discussing recent research on the subject. The papers focus on critical thinking and culture, historical consciousness and critical thinking, critical thinking as cultural-historical practice, culture and the development of critical…

  20. Determinants of Extraocular Muscle Volume in Patients with Graves' Disease

    Directory of Open Access Journals (Sweden)

    Samer El-Kaissi

    2012-01-01

    Full Text Available Background. To examine factors contributing to extraocular muscle (EOM volume enlargement in patients with Graves’ hyperthyroidism. Methods. EOM volumes were measured with orbital magnetic resonance imaging (MRI in 39 patients with recently diagnosed Graves’ disease, and compared to EOM volumes of 13 normal volunteers. Thyroid function tests, uptake on thyroid scintigraphy, anti-TSH-receptor antibody positivity and other parameters were then evaluated in patients with EOM enlargement. Results. 31/39 patients had one or more enlarged EOM, of whom only 2 patients had clinical EOM dysfunction. Compared to Graves’ disease patients with normal EOM volumes, those with EOM enlargement had significantly higher mean serum TSH (0.020±0.005 versus 0.007±0.002 mIU/L; P value 0.012, free-T4 (52.9±3.3 versus 41.2±1.7 pmol/L; P value 0.003 and technetium uptake on thyroid scintigraphy (13.51±1.7% versus 8.55±1.6%; P value 0.045. There were no differences between the 2 groups in anti-TSH-receptor antibody positivity, the proportion of males, tobacco smokers, or those with active ophthalmopathy. Conclusions. Patients with recently diagnosed Graves’ disease and EOM volume enlargement have higher serum TSH and more severe hyperthyroidism than patients with normal EOM volumes, with no difference in anti-TSH-receptor antibody positivity between the two groups.

  1. Reliability of brain volume measurements: a test-retest dataset.

    Science.gov (United States)

    Maclaren, Julian; Han, Zhaoying; Vos, Sjoerd B; Fischbein, Nancy; Bammer, Roland

    2014-01-01

    Evaluation of neurodegenerative disease progression may be assisted by quantification of the volume of structures in the human brain using magnetic resonance imaging (MRI). Automated segmentation software has improved the feasibility of this approach, but often the reliability of measurements is uncertain. We have established a unique dataset to assess the repeatability of brain segmentation and analysis methods. We acquired 120 T1-weighted volumes from 3 subjects (40 volumes/subject) in 20 sessions spanning 31 days, using the protocol recommended by the Alzheimer's Disease Neuroimaging Initiative (ADNI). Each subject was scanned twice within each session, with repositioning between the two scans, allowing determination of test-retest reliability both within a single session (intra-session) and from day to day (inter-session). To demonstrate the application of the dataset, all 3D volumes were processed using FreeSurfer v5.1. The coefficient of variation of volumetric measurements was between 1.6% (caudate) and 6.1% (thalamus). Inter-session variability exceeded intra-session variability for lateral ventricle volume (P<0.0001), indicating that ventricle volume in the subjects varied between days.

  2. Solvent-free Synthesis of Novel N-substituted R-oxycarbonyl 1,6-Methano [10] annulene-3,4-dicarboximide%新型N-取代-R-氧基羰基-1,6-亚甲基桥[10]轮烯-3,4-二甲酰亚胺的无溶剂合成

    Institute of Scientific and Technical Information of China (English)

    张若思; 刘建军; 屈莹; 左胜利

    2013-01-01

    1,6-Methano [10] annulene-3,4-dicarboximide (2) was synthesized from 1,6-methano [10] annulene-3,4-dicarboxylic anhydride with urea.2 reacted with a potassium hydroxide solution in methanol to obtain potassium 1,6-methano[10] annulene-3,4-dicarboximide (3).Four novel N-substituted R-oxycarbonyl 1,6-methano [10] annulene-3,4-dicarboximides were synthesized by reaction of 3 with chloroformate ester in solvent-free.The structures were characterized by 1H NMR,13C NMR,IR,MS and HR-MS.%在无溶剂条件下,1,6-亚甲基桥[10]轮烯-3,4-二甲酸酐与尿素反应制得1,6-亚甲基桥[10]轮烯-3,4-二甲酰亚胺(2);2与KOH的甲醇溶液于常温反应得1,6-亚甲基桥[10]轮烯-3,4-二甲酰亚胺钾(3);3与氯甲酸酯反应合成了4个新型的N-酯基取代的1,6-亚甲基桥[10]轮烯-3,4-二甲酰亚胺,其结构经1H NMR,13C NMR,IR,MS和HR-MS表征.

  3. Magnetic Phase Transition and Magnetic Entropy Change in La0.8Pr0.2Fe11.4Si1.6 Compound

    Institute of Scientific and Technical Information of China (English)

    Li Xiaowei; Li Guodong; Xu Chao; Wang Ligang

    2007-01-01

    The magnetic properties and the phase transformation of the partial substitution of Pr for La in LaFe11.4Si1.6 have been investigated by the means of X-ray diffraction (XRD) and vibrating sample magnetic (VSM). The results indicated that the single phase NaZn13-type cubic structure is stabilized for the compound La0.8Pr0.2Fe11.4Si1.6 and large values of the isothermal magnetic entropy change △SM around the curie temperature Tc~194 K in relative low magnetic fields. The maximum value |△SM|max~37.07J/kg·K-1 under a field of 1.5 T. Such large MCEs are attributed to the sharp change of the magnetization at the Curie temperature, the field-induced IEM transition and a strong temperature dependence of the critical field BC.

  4. Target and Beam-Target Spin Asymmetries in Exclusive $\\pi^+$ and $\\pi^-$ Electroproduction with 1.6 to 5.7 GeV Electrons

    CERN Document Server

    Bosted, P E; Careccia, S; Dodge, G; Fersch, R; Kuhn, S E; Pierce, J; Prok, Y; Zheng, X; Adhikari, K P; Adikaram, D; Akbar, Z; Amaryan, M J; Pereira, S Anefalos; Asryan, G; Avakian, H; Badui, R A; Ball, J; Baltzell, N A; Battaglieri, M; Batourine, V; Bedlinskiy, I; Boiarinov, S; Briscoe, W J; Bültmann, S; Burkert, V D; Cao, T; Carman, D S; Celentano, A; Chandavar, S; Charles, G; Chetry, T; Ciullo, G; Clark, L; Colaneri, L; Cole, P L; Contalbrigo, M; Cortes, O; Crede, V; D'Angelo, A; Dashyan, N; De Vita, R; Deur, A; Djalali, C; Dupre, R; Egiyan, H; Alaoui, A El; Fassi, L El; Eugenio, P; Fanchini, E; Fedotov, G; Filippi, A; Fleming, J A; Forest, T A; Fradi, A; Garçon, M; Gevorgyan, N; Ghandilyan, Y; Gilfoyle, G P; Giovanetti, K L; Girod, F X; Gleason, C; Gohn, W; Golovatch, E; Gothe, R W; Griffioen, K A; Guo, L; Hafidi, K; Hanretty, C; Harrison, N; Hattawy, M; Heddle, D; Hicks, K; Holtrop, M; Hughes, S M; Ilieva, Y; Ireland, D G; Ishkhanov, B S; Isupov, E L; Jenkins, D; Jiang, H; Jo, H S; Joo, K; Joosten, S; Keller, D; Khandaker, M; Kim, W; Klein, A; Klein, F J; Kubarovsky, V; Kuleshov, S V; Lanza, L; Lenisa, P; Livingston, K; Lu, H Y; MacGregor, I J D; Markov, N; McCracken, M E; McKinnon, B; Meyer, C A; Minehart, R; Mirazita, M; Mokeev, V; Movsisyan, A; Munevar, E; Camacho, C Munoz; Nadel-Turonski, P; Net, L A; Ni, A; Niccolai, S; Niculescu, G; Niculescu, I; Osipenko, M; Ostrovidov, A I; Paremuzyan, R; Park, K; Pasyuk, E; Peng, P; Phelps, W; Pisano, S; Pogorelko, O; Price, J W; Procureur, S; Protopopescu, D; Puckett, A J R; Raue, B A; Ripani, M; Rizzo, A; Rosner, G; Rossi, P; Roy, P; Sabatié, F; Salgado, C; Schumacher, R A; Seder, E; Sharabian, Y G; Simonyan, A; Skorodumina, Iu; Smith, G D; Sparveris, N; Stankovic, Ivana; Stepanyan, S; Strakovsky, I I; Strauch, S; Sytnik, V; Taiuti, M; Tian, Ye; Torayev, B; Ungaro, M; Voskanyan, H; Voutier, E; Walford, N K; Watts, D P; Wei, X; Weinstein, L B; Wood, M H; Zachariou, N; Zana, L; Zhang, J; Zhao, Z W; Zonta, I

    2016-01-01

    Beam-target double spin asymmetries and target single-spin asymmetries in exclusive $\\pi^+$ and $\\pi^-$ electroproduction were obtained from scattering of 1.6 to 5.7 GeV longitudinally polarized electrons from longitudinally polarized protons (for $\\pi^+$) and deuterons (for $\\pi^-$) using the CEBAF Large Acceptance Spectrometer (CLAS) at Jefferson Lab. The kinematic range covered is $1.11.5$ GeV. Very large target-spin asymmetries are observed for $W>1.6$ GeV. When combined with cross section measurements, the present results will provide powerful constraints on nucleon resonance amplitudes at moderate and large values of $Q^2$, for resonances with masses as high as 2.3 GeV.

  5. Effects of dietary β-1,3/1,6-glucan on the antioxidant and digestive enzyme activities of Pacific red snapper (Lutjanus peru) after exposure to lipopolysaccharides.

    Science.gov (United States)

    Guzmán-Villanueva, Laura T; Ascencio-Valle, Felipe; Macías-Rodríguez, María E; Tovar-Ramírez, Dariel

    2014-06-01

    The effect of β-1,3/1,6-glucan, derived from yeast, on growth, antioxidant, and digestive enzyme performance of Pacific red snapper Lutjanus peru before and after exposure to lipopolysaccharides (LPS) was investigated. The β-1,3/1,6-glucan was added to the basal diet at two concentrations (0.1 and 0.2 %). The treatment lasted 6 weeks, with sampling at regular intervals (0, 2, 4, and 6 weeks). At the end of this period, the remaining fish from either control or β-glucan-fed fish were injected intraperitoneally with LPS (3 mg kg(-1)) or with sterile physiological saline solution (SS) and then sampled at 0, 24, and 72 h. The results showed a significant increase (P snapper.

  6. Structure and composition of the superconducting phase in alkali iron selenide KyFe1.6+xSe2

    Science.gov (United States)

    Carr, Scott V.; Louca, Despina; Siewenie, Joan; Huang, Q.; Wang, Aifeng; Chen, Xianhui; Dai, Pengcheng

    2014-04-01

    We use neutron diffraction to study the temperature evolution of the average structure and local lattice distortions in insulating and superconducting potassium iron selenide KyFe1.6+xSe2. In the high temperature paramagnetic state, both materials have a single phase with a crystal structure similar to that of the BaFe2As2 family of iron pnictides. While the insulating KyFe1.6+xSe2 forms a √5 ×√5 iron vacancy ordered block antiferromagnetic (AF) structure at low temperature, the superconducting compounds spontaneously phase separate into an insulating part with √5 ×√5 iron vacancy order and a superconducting phase with chemical composition of KzFe2Se2 and BaFe2As2 structure. Therefore, superconductivity in alkaline iron selenides arises from alkali deficient KzFe2Se2 in the matrix of the insulating block AF phase.

  7. Improvement in elastic properties of CuAl{sub 0.4}Fe{sub 1.6}O{sub 4} spinel ferrite by rapid thermal cooling

    Energy Technology Data Exchange (ETDEWEB)

    Modi, K. B., E-mail: kunalbmodi2003@yahoo.com; Shah, S. J., E-mail: kunalbmodi2003@yahoo.com; Pathak, T. K., E-mail: kunalbmodi2003@yahoo.com; Vasoya, N. H., E-mail: kunalbmodi2003@yahoo.com; Lakhani, V. K., E-mail: kunalbmodi2003@yahoo.com [Department of Physics, Saurashtra University, Rajkot-360005 (India); Yahya, A. K. [School of Physics and Materials Studies, University Technology MARA, 40450 Shah Alam, Selangor (Malaysia)

    2014-04-24

    The elastic properties of spinel ferrite composition, CuAl{sub 0.4}Fe{sub 1.6}O{sub 4}, quenched from final sintering temperature of 1373 K to liquid nitrogen temperature (∼ 80K) have been studied by means of X-ray powder diffractometry and pulse echo-overlap technique (9 MHz) at 300 K. The magnitude of elastic constants is found to enhance by 15% compared to slowly-cooled counterpart. The observed mechanical strengthening has been discussed in the light of compressive stress on the surface, with tensile stresses at interior regions and corresponding changes in structural parameters. The B{sub o}/G{sub o} ratio indicates the brittle nature of CuAl{sub 0.4}Fe{sub 1.6}O{sub 4}.

  8. Postoperative volume balance

    DEFF Research Database (Denmark)

    Frost, H; Mortensen, C.R.; Secher, Niels H.

    2017-01-01

    In healthy humans, stroke volume (SV) and cardiac output (CO) do not increase with expansion of the central blood volume by head-down tilt or administration of fluid. Here, we exposed 85 patients to Trendelenburg's position about one hour after surgery while cardiovascular variables were determined...... non-invasively by Modelflow. In Trendelenburg's position, SV (83 ± 19 versus 89 ± 20 ml) and CO (6·2 ± 1·8 versus 6·8 ± 1·8 l/min; both Pheart rate (75 ± 15 versus 76 ± 14 b min(-1) ) and mean arterial pressure were unaffected (84 ± 15 versus 84 ± 16 mmHg). For the 33 patients......, determination of SV and/or CO in Trendelenburg's position can be used to evaluate whether a patient is in need of IV fluid as here exemplified after surgery....

  9. Charged Pion Form Factor Determination in the Range of Q2 = 0.6 ~ 1.6 (GeV/c)2

    Institute of Scientific and Technical Information of China (English)

    Nader Ghahramany; Kamran Rostami; Mohammad Ghanatian

    2004-01-01

    Using the most recent differential cross section data for ep quasi-elastic scattering, the charged pion formation and its form factor Fπ is calculated in the energy range of 2.4 ~ 4 GeV at Q2 = 0.6 ~ 1.6 (GeV/c)2. The functional dependence of the charged pion form factor to the separated cross section σL is investigated and compared to the previously determined result.

  10. On the origin of the two thermally driven relaxations in diluted spin ice Dy(1.6)Y(0.4)Ti2O7.

    Science.gov (United States)

    Xing, Hui; Guo, Hanjie; Feng, Chunmu; Xu, Zhu-An; Zeng, Hao

    2013-01-30

    We report quantitative analysis of the ac susceptibility of the diluted spin ice compound Dy(1.6)Y(0.4)Ti(2)O(7), by fitting the frequency spectra of the two observed relaxation modes with a double Cole-Cole formula. Our results suggest that both modes are thermally activated, with the same intrinsic frequency, but different activation barriers. We propose that these thermally activated relaxation modes can be attributed to correlated spin clusters.

  11. Synthesis, Dimeric Crystal Structure, and Biological Activities of N-(4-Methyl-6-oxo-1,6-dihydro-pyrimidin-2-yl)-N-(2-trifluoromethyl-phenyl)-guanidine

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    The title compound, N-(4-methyl-6-oxo-1,6-dihydro-pyrimidin-2-yl)-N′-(2-trifluoromethyl-phenyl)-guanidine, was synthesized and its structure was confirmed by using IR, MS, 1H NMR, and elemental analysis. The single crystal structure of the title compound was determined by X-ray diffraction. The preliminary biological test showed that the synthesized compound has a weak herbicidal activity.

  12. Introduction: Envisioning Future War; Strategic Insights, v. 10, Special issue (October 2011), 1-6. Topic: Global Trends and Future Warfare

    OpenAIRE

    Moran, Daniel

    2011-01-01

    This article appeared in Strategic Insights, v.10, Special issue (October 2011), 1-6. Topic: Global Trends and Future Warfare Approved for public display, distribution unlimited The workshop whose proceedings are presented here was convened to provide the National Intelligence Council (NIC) with insight into the way war in the intermediate future, meaning the next twenty years or so, is viewed from the perspective of America's allies, partners, and potential adversaries. The group took ...

  13. The moonlighting protein fructose-1, 6-bisphosphate aldolase of Neisseria meningitidis: surface localization and role in host cell adhesion

    OpenAIRE

    Tunio, Sarfraz A; Oldfield, Neil James; Berry, Alan; Ala'Aldeen, Dlawer AA; Wooldridge, Karl G.; Turner, David PJ

    2010-01-01

    Abstract Fructose-1, 6-bisphosphate aldolases (FBA) are cytoplasmic glycolytic enzymes, which despite lacking identifiable secretion signals, have also been found localized to the surface of several bacteria where they bind host molecules and exhibit non-glycolytic functions. Neisseria meningitidis is an obligate human nasopharyngeal commensal, which has the capacity to cause life-threatening meningitis and septicemia. Recombinant native N. meningitidis FBA was purified and used in...

  14. Production yields of {sup Nat}Zn(p,x){sup 67}Ga reaction in the energy range of 1.6 to 2.5 MeV

    Energy Technology Data Exchange (ETDEWEB)

    Wachter, J A; Miranda, P A; Cancino, S A; Morales, J R; Dinator, M I [Facultad de Ciencias, Universidad de Chile, Casilla 653, Santiago (Chile)], E-mail: javier.wachter@gmail.com

    2008-11-01

    Production yield of the {sup 67}Ga (t{sub 1/2} = 78.3hr.)radionuclide was measured by the {sup Nat}Zn(p,x){sup 67} Ga reaction in the energy range from 1.6 to 2.5 MeV. These results are the first reported at energies under 3 MeV. The overall uncertainty of these measurements are around 7%.

  15. Organocatalytic one-pot 1,4-/1,6-/1,2-addition sequence for the stereocontrolled formation of six consecutive stereocenters.

    Science.gov (United States)

    Chauhan, Pankaj; Mahajan, Suruchi; Raabe, Gerhard; Enders, Dieter

    2015-02-11

    An unprecedented stereoselective organocatalytic one-pot 1,4-/1,6-/1,2-addition sequence between β-dicarbonyl compounds, β-nitroalkenes and 4-nitro-5-styrylisoxazoles sequentially catalyzed by low loading of a squaramide catalyst and an achiral base has been developed. The protocol opens an efficient entry to isoxazole bearing cyclohexanes with six consecutive stereogenic centers including one tetrasubstituted carbon in good yields and excellent diastereo- and enantioselectivities.

  16. Divergent actions of the pyrethroid insecticides S-bioallethrin, tefluthrin, and deltamethrin on rat Na(v)1.6 sodium channels.

    Science.gov (United States)

    Tan, Jianguo; Soderlund, David M

    2010-09-15

    We expressed rat Na(v)1.6 sodium channels in combination with the rat beta(1) and beta(2) auxiliary subunits in Xenopus laevis oocytes and evaluated the effects of the pyrethroid insecticides S-bioallethrin, deltamethrin, and tefluthrin on expressed sodium currents using the two-electrode voltage clamp technique. S-Bioallethrin, a type I structure, produced transient modification evident in the induction of rapidly decaying sodium tail currents, weak resting modification (5.7% modification at 100 microM), and no further enhancement of modification upon repetitive activation by high-frequency trains of depolarizing pulses. By contrast deltamethrin, a type II structure, produced sodium tail currents that were ~9-fold more persistent than those caused by S-bioallethrin, barely detectable resting modification (2.5% modification at 100 microM), and 3.7-fold enhancement of modification upon repetitive activation. Tefluthrin, a type I structure with high mammalian toxicity, exhibited properties intermediate between S-bioallethrin and deltamethrin: intermediate tail current decay kinetics, much greater resting modification (14.1% at 100 microM), and 2.8-fold enhancement of resting modification upon repetitive activation. Comparison of concentration-effect data showed that repetitive depolarization increased the potency of tefluthrin approximately 15-fold and that tefluthrin was approximately 10-fold more potent than deltamethrin as a use-dependent modifier of Na(v)1.6 sodium channels. Concentration-effect data from parallel experiments with the rat Na(v)1.2 sodium channel coexpressed with the rat beta(1) and beta(2) subunits in oocytes showed that the Na(v)1.6 isoform was at least 15-fold more sensitive to tefluthrin and deltamethrin than the Na(v)1.2 isoform. These results implicate sodium channels containing the Na(v)1.6 isoform as potential targets for the central neurotoxic effects of pyrethroids.

  17. Clearance effect of sandblasting and hand scaling on subgingival plaque and their effect on interleukin 1/6/8/10 and MMP8/TIMP1 levels

    Institute of Scientific and Technical Information of China (English)

    Lei Li

    2016-01-01

    Objective:To study the clearance effect of sandblasting and hand scaling on subgingival plaque and their effect on interleukin 1/6/8/10 and MMP8/TIMP1 levels.Methods: A total of 40 patients with subgingival plaque were selected for study, subgingival plaque of left half mouth received hand scaling and was included in hand scaling group, subgingival plaque of right half mouth received sandblasting scaling and was included in sandblasting group, probing depth (PD) and bleeding on probing (BOP) were assessed and the percentage of closed periodontal pocket was calculate before and after treatment; gingival crevicular fluid was collected after treatment to determine interleukin 1/6/8/10 and MMP8/TIMP1 levels.Results:7 d and 30 d after treatment, PD and BOP percentage of both groups were lower than those before treatment and the percentage of closed periodontal pocket were higher than those before treatment, the PD and BOP percentage of sandblasting group after treatment were lower than those of hand scaling group and the percentage of closed periodontal pocket was higher than that of hand scaling group; 7 d after treatment, IL-1, -6, -8 and MMP8 levels and MMP8/TIMP1 ratio in gingival crevicular fluid of sandblasting group were significantly lower than those of hand scaling group while IL-10 and TIMP1 levels were significantly higher than those of hand scaling group.Conclusion:Sandblasting scaling has better clearance effect on subgingival plaque than hand scaling, and can adjust the interleukin 1/6/8/10 and MMP8/TIMP1 levels to relieve periodontal tissue inflammation and alveolar bone resorption.

  18. Synthesis of All-Z-1,6,9,12,15-Octadecapenten-3-one, A Vinyl Ketone Polyunsaturated Marine Natural Product Isolated from Callysponga sp.

    Directory of Open Access Journals (Sweden)

    Anne Marie Langseter

    2014-03-01

    Full Text Available The synthesis of the marine natural product 1,6Z,9Z,12Z,15Z-octadecapentaen-3-one (1 has been achieved by two different routes starting from the ethyl esters of eicosapentaenoic acid (EPA and docosahexaenoic acid (DHA, respectively. Using EPA ethyl ester as starting material the polyunsaturated vinyl ketone lipid 1 was obtained in 17% overall yield.

  19. SYNTHESIS AND CHARACTERIZATION OF LIQUID CRYSTALLINE MULTI-BLOCK COPOLYMERS,POLY[1,6-BIS(4-OXYBENZOYL-OXY)HEXANE TEREPHTHALATE]-b-BISPHENOL A POLYCARBONATE

    Institute of Scientific and Technical Information of China (English)

    Hui-qing Zhang; Xiong-yan Zhao; De-shan Liu; Qi-xiang Zhou

    1999-01-01

    A series of liquid crystalline multi-block copolymers poly[1,6-bis(4-oxybenzoyl-oxy)hexane terephthalate]-b-bisphenol A polycarbonate (PHTH-6-b-PC) with different segment lengths were synthesized in tetrachloroethane by solution polycondensation in which hydroxyl terminated PC and acyl chloride terminated PHTH-6 were used. It is found that block copolymers with high molecular weight and welldefined structures were obtained. All the block copolymers exhibit a nematic liquid crystalline texture.

  20. Identification of schistosoma mansoni antigens recognised by spleen cells of C57B1/6 mice immunized with ultraviolet-irradiated cercariae

    Energy Technology Data Exchange (ETDEWEB)

    Osman, A.; El Ridi, R. (Cairo Univ. (Egypt)); Guirguis, N. (VACSERA, El Agouza Cairo (Egypt). Biomedical Research Dept.); Dean, D.A. (U.S. Naval Medical Research Unit No. 3, Cairo (Egypt))

    1994-11-01

    Spleen cells of C57B1/6 mice immunized twice with Schistosoma mansoni cercariae attenuated by ultraviolet irradiation proliferated and produced interleukin-(I1)-2 and/or I1-4 in response to both soluble schistosomular and adult worm antigens of 72-68, 60-62, 50, 45, 29.5 and 28 kDa. All of these bands, except the 45 kDa, were also recognized by serum antibodies in Western blotting. (author).

  1. Degradation studies of 1, 6-diisocyanatohexane-extended poly (1, 4-butylene succinate) - bioactive glass scaffolds for bone tissue repair applications

    Science.gov (United States)

    Kaur, Kulwinder; Singh, K. J.; Anand, Vikas

    2016-05-01

    Bio composite scaffolds prepared from polymer and bio glass provide necessary sites for bone tissue regeneration. In the presented work, bioactive glass scaffolds have been prepared from 1, 6-diisocyanatohexane-extended poly (1, 4-butylene succinate) with different amount of bioactive glass powder by solvent casting method. Prepared scaffolds have been characterized by XRD, FTIR and FESEM techniques. Effect of content of bioactive glass on biodegradability has been investigated in detail.

  2. Select Papers. Volume 1

    Science.gov (United States)

    2011-08-01

    non- uniform rational B-splines (NURBS), 127 and BRL-CAD TM format. This dual-package development allowed for rapid development of components ...next generation of scientists and engineers. A fundamental component of our outreach program is to provide students research experiences at ARL...summer intern. There, I ran Volume Based Morphometry , an application of Statistical Parametric Mapping that was new to the Hirsch lab. I

  3. Small-Volume Injections: Evaluation of Volume Administration Deviation From Intended Injection Volumes.

    Science.gov (United States)

    Muffly, Matthew K; Chen, Michael I; Claure, Rebecca E; Drover, David R; Efron, Bradley; Fitch, William L; Hammer, Gregory B

    2017-10-01

    In the perioperative period, anesthesiologists and postanesthesia care unit (PACU) nurses routinely prepare and administer small-volume IV injections, yet the accuracy of delivered medication volumes in this setting has not been described. In this ex vivo study, we sought to characterize the degree to which small-volume injections (≤0.5 mL) deviated from the intended injection volumes among a group of pediatric anesthesiologists and pediatric postanesthesia care unit (PACU) nurses. We hypothesized that as the intended injection volumes decreased, the deviation from those intended injection volumes would increase. Ten attending pediatric anesthesiologists and 10 pediatric PACU nurses each performed a series of 10 injections into a simulated patient IV setup. Practitioners used separate 1-mL tuberculin syringes with removable 18-gauge needles (Becton-Dickinson & Company, Franklin Lakes, NJ) to aspirate 5 different volumes (0.025, 0.05, 0.1, 0.25, and 0.5 mL) of 0.25 mM Lucifer Yellow (LY) fluorescent dye constituted in saline (Sigma Aldrich, St. Louis, MO) from a rubber-stoppered vial. Each participant then injected the specified volume of LY fluorescent dye via a 3-way stopcock into IV tubing with free-flowing 0.9% sodium chloride (10 mL/min). The injected volume of LY fluorescent dye and 0.9% sodium chloride then drained into a collection vial for laboratory analysis. Microplate fluorescence wavelength detection (Infinite M1000; Tecan, Mannedorf, Switzerland) was used to measure the fluorescence of the collected fluid. Administered injection volumes were calculated based on the fluorescence of the collected fluid using a calibration curve of known LY volumes and associated fluorescence.To determine whether deviation of the administered volumes from the intended injection volumes increased at lower injection volumes, we compared the proportional injection volume error (loge [administered volume/intended volume]) for each of the 5 injection volumes using a linear

  4. Witnessing the assembly at z=1.6 of a galaxy cluster (GMASS ultradeep spectroscopy of galaxies at z~2 - V)

    CERN Document Server

    Kurk, J; Zamorani, G; Halliday, C; Mignoli, M; Pozzetti, L; Daddi, E; Rosati, P; Dickinson, M; Bolzonella, M; Cassata, P; Renzini, A; Franceschini, A; Rodighiero, G; Berta, S

    2009-01-01

    We present a study of a galaxy overdensity at z=1.6, Cl 0332-2742, uncovered in the Galaxy Mass Assembly ultra-deep Spectroscopic Survey (GMASS). We conservatively estimate the overdensity in redshift space for the spike, containing 42 galaxies at z=1.6, to be 8.3+/-1.5 and find a velocity dispersion of 500 km/s. A map of the surface density of galaxies at z=1.6 in the GMASS field shows that its structure is irregular. The differences in the physical properties of Cl 0332-2742 member and field galaxies agree with the latest hierarchical galaxy formation models: for overdensity members, the star formation rate (SFR), and specific SFR, is approximately 50% lower than for the field galaxies; overdensity galaxies are twice the age, on average, of field galaxies; and there is a higher proportion of both massive (M>10^{10.7} M_sol), and early-type galaxies, inside Cl 0332-2742 than in the field. Among the 42 members, 7 have spectra consistent with being passively evolving, massive galaxies. In a z-J colour-magnitud...

  5. Rapid and accurate detection of the CFTR gene mutation 1811+1.6 kbA>G by real-time fluorescence resonance energy transfer PCR.

    Science.gov (United States)

    Reboul, Marie-Pierre; Higueret, Laurent; Biteau, Nicolas; Iron, Albert

    2005-10-01

    The CFTR gene mutation 1811+1.6 kbA>G has been reported as associated with a severe phenotype of cystic fibrosis with pancreatic insufficiency. This mutation has been identified as a rather common one in the South West of France and in the Iberian Peninsula. Because of the precise geographical origin of the subjects and its frequency, the mutation has to be investigated with accuracy. We have developed an original real-time Fluorescence Resonance Energy Transfer (FRET) PCR assay for genotyping the mutation 1811+1.6 kbA>G. It is based on the amplification of a region spanning the mutation with simultaneous detection of the amplicon by hybridization with a bi-probe followed by a melting curve analysis. The results obtained are identical with those resulting from either restriction fragment length polymorphism analysis or sequencing. The distinction between the wild type and the mutation 1811+1.6 kbA>G is easy because the corresponding melting points shows a difference of 6 or 9.5 degrees C depending on the associated SNP A/T located 16 bp downstream. We demonstrated that a FRET assay showed enough sensitivity to discriminate between two nucleotide polymorphisms (SNPs) in the sequence of the sensor. In conclusion, this method is specific, fast, easy to perform, reproducible, inexpensive as it uses only one bi-probe and well adapted to daily practice.

  6. Reversal effect of GnT-V on the radioresistance of human nasopharyngeal carcinoma cells by alteration β1, 6-GlcNAc branched N-glycans.

    Science.gov (United States)

    Wu, Jun-Bo; Shen, Li; Qiu, Li; Duan, Qi-Wen; Luo, Zhi-Guo; Dong, Xiao-Xia

    2015-01-01

    Radiotherapy is the primary treatment for human nasopharyngeal carcinoma (NPC), yet radioresistance remains a major obstacle to successful treatment in many cases. N-acetylglucosaminyltransferase V (GnT-V), which synthesizes β1, 6-GlcNAc branched N-glycans, is closely related to the radiosensitivity of NPC cells. However, a better understanding of the functional role of GnT-V in NPC radioresistance and the related mechanisms is urgently needed. In the present study, a radioresistant NPC cell line, CNE-2R, was established by repeated γ-irradiation. We found that GnT-V levels, as well as β1, 6-GlcNAc branched N-glycans were significantly increased in the CNE-2R cells as compared with that in the parental cells. Meanwhile, knockdown of GnT-V in the CNE-2R cells enhanced cell radiosensitivity and inhibited the formation of β1, 6-branched N-glycans. In addition, the regulated expression of GnT-V in the CNE-2R cells converted the heterogeneous N-glycosylated forms of CD147. Furthermore, swainsonine, an inhibitor of N-glycan biosynthesis, was also able to reverse the radioresistance of the CNE-2R cells. Taken together, the present study revealed a novel mechanism of GnT-V as a regulator of radioresistance in NPC cells, which may be useful for fully understanding the biological role of N-glycans in NPC radioresistance.

  7. 电场作用下1.6%C-UHCS/40Cr钢的超塑性焊接

    Institute of Scientific and Technical Information of China (English)

    张柯柯; 孙敬; 吴志伟; 马宁; 邱然锋; 张占领

    2011-01-01

    基于对40Cr钢淬火后与经热机械处理超高碳钢1.6% C-UHCS电场作用下超塑性焊接可行性的分析,在非真空、无保护气氛下,进行了电场作用下1.6% C-UHCS/40Cr的超塑性焊接工艺试验.结果表明,当施加试样接正极、环状电极接负极的电场时,可明显提高40Cr钢淬火后与经热机械处理超高碳钢1.6% C-UHCS的超塑性焊接效果.与不加电场相比,当两者在外加电场+3 kV/cm、预压应力56.6 MPa、焊接温度780℃、初始应变速率1.5×10-4/s的条件下,经20 min超塑性焊接,接头强度提高26.8%.

  8. Analgesic efficacy of local anaesthetic wound administration in knee arthroplasty: volume vs concentration

    DEFF Research Database (Denmark)

    Andersen, L Ø; Gaarn-Larsen, L; Kristensen, B B;

    2010-01-01

    Wound administration of local anaesthetic may be effective for postoperative pain management in knee arthroplasty, but the analgesic efficacy of local anaesthetic in relation to volume vs concentration has not been determined. In a double-blinded trial, 48 patients scheduled for total knee...... arthroplasty were randomly assigned to receive either a high volume/low concentration solution of ropivacaine (20 ml, 0.5%) or a low volume/high concentration solution of ropivacaine (10 ml, 1%), 6 and 24 h postoperatively through an intracapsular catheter. Pain was assessed for 2 h after administration. Pain...

  9. Analgesic efficacy of local anaesthetic wound administration in knee arthroplasty: volume vs concentration

    DEFF Research Database (Denmark)

    Andersen, L Ø; Gaarn-Larsen, L; Kristensen, B B;

    2010-01-01

    Wound administration of local anaesthetic may be effective for postoperative pain management in knee arthroplasty, but the analgesic efficacy of local anaesthetic in relation to volume vs concentration has not been determined. In a double-blinded trial, 48 patients scheduled for total knee...... arthroplasty were randomly assigned to receive either a high volume/low concentration solution of ropivacaine (20 ml, 0.5%) or a low volume/high concentration solution of ropivacaine (10 ml, 1%), 6 and 24 h postoperatively through an intracapsular catheter. Pain was assessed for 2 h after administration. Pain...... was reduced in both groups with ropivacaine administration 24 h postoperatively (p

  10. Modification to the Remedial Action Plan and site design for stabilization of the inactive uranium mill tailings site at Mexican Hat, Utah: Volume 1, Text, Attachments 1--6. Final report

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1989-01-01

    This document provides the modifications to the 1988 Remedial Action Plan (RAP) of the contaminated materials at the Monument Valley, Arizona, and Mexican Hat, Utah. The text detailing the modifications and attachments 1 through 6 are provided with this document. The RAP was developed to serve a two-fold purpose. It presents the activities proposed by the Department of Energy (DOE) to accomplish long-term stabilization and control of the residual radioactive materials (RRM) from Monument Valley, Arizona, and Mexican Hat, Utah, at the Mexican Hat disposal site. It also serves to document the concurrence of both the Navajo Nation and the Nuclear Regulatory Commission (NRC) in the remedial action. This agreement, upon execution by DOE and the Navajo Nation and concurrence by the NRC, becomes Appendix B of the Cooperative Agreement. This document has been structured to provide a comprehensive understanding of the remedial action proposed for the Monument Valley and Mexican Hat sites. It includes specific design and construction requirements for the remedial action. Pertinent information and data are included with reference given to the supporting documents.

  11. Dietary administration of β-1,3/1,6-glucan and probiotic strain Shewanella putrefaciens, single or combined, on gilthead seabream growth, immune responses and gene expression.

    Science.gov (United States)

    Guzmán-Villanueva, Laura T; Tovar-Ramírez, Dariel; Gisbert, Enric; Cordero, Héctor; Guardiola, Francisco A; Cuesta, Alberto; Meseguer, José; Ascencio-Valle, Felipe; Esteban, Maria A

    2014-07-01

    It is widely known that β-glucans and probiotic bacteria are good immunostimulants for fish. In the present work we have evaluated the dietary effect of β-1,3/1,6-glucan (isolated from Laminarina digitata) and Pdp 11 (Shewanella putrefaciens, probiotic isolated from gilthead seabream skin), single or combined, on growth, humoural (seric level of total IgM antibodies and peroxidase and antiprotease activities) and cellular innate immune response (peroxidase and phagocytic activities of head-kidney leucocytes), as well as the expression of immune-related genes in gilthead seabream (Sparus aurata). Four treatment groups were established: control (non-supplemented diet), Pdp 11 (10(9) cfu g(-1)), β-1,3/1,6-glucan (0.1%) and β-1,3/1,6-glucan + Pdp 11 (0.1% and 10(9) cfu g(-1), respectively). Fish were sampled after 1, 2 and 4 weeks of feeding. Interestingly, all supplemented diets produced increments in the seabream growth rates, mainly the Pdp 11-suplemented diet. Overall, Pdp 11 dietary administration resulted in decreased serum IgM levels and peroxidase activity. However, the seric antiprotease activity was increased in fish fed with both supplements together. Furthermore, β-1,3/1,6-glucan and combined diet increased phagocytic activity after 2 or 4 weeks. At gene level, IL-1β and INFγ transcripts were always up-regulated in HK but only the interleukin reached significance after 4 weeks in the group fed with β-glucan. On the contrary, IgM gene expression tended to be down-regulated being significant after 1 week in seabream specimens fed with β-glucan or β-glucan plus Pdp 11. These results suggest that β-1,3/1,6-glucan and Pdp 11 modulate the immune response and stimulates growth of the gilthead seabream, one of the species with the highest rate of production in Mediterranean aquaculture. Copyright © 2014 Elsevier Ltd. All rights reserved.

  12. Ag doped (Bi1.6Pb0.4Sr2CaCu2O8+δ textured rods

    Directory of Open Access Journals (Sweden)

    Díez, J. C.

    2008-06-01

    Full Text Available In this work, superconducting samples of (Bi1.6Pb0.4Sr2CaCu2O8+δ with Ag additions have been studied. (Bi1.6Pb0.4Sr2CaCu2O8+δ + x wt.% Ag (with x = 0, 1 and 3 powders were synthesized using a sol-gel method. The obtained powders were used as precursors to fabricate long textured cylindrical bars through a floating zone melting method. A drastic change on the microstructure has been found when comparing with undoped Bi2Sr2CaCu2O8+δ samples. The results showed that electrical resistivity at room temperature, critical current as well as flexural strength are improved when Ag is added to these Pb doped samples, while critical temperature does not change. On the other hand, it has been found that samples with composition (Bi1.6Pb0.4Sr2CaCu2O8+δ + Ag shown E-I curves with very high sharpness values on the zone of the superconducting to normal transition, reaching n-values (E∼In as high as 45 at 65K.Se han preparado polvos cerámicos de composición (Bi1.6Pb0.4Sr2CaCu2O8+δ + x % Ag en peso (con x = 0, 1 y 3 mediante un proceso sol-gel. Estos polvos se han utilizado para fabricar precursores que se texturaron por medio del método de fusión zonal flotante. Se ha encontrado un gran cambio en la microestructura cuando se compara con muestras de composición pura Bi2Sr2CaCu2O8+δ. Tanto la resistividad eléctrica a temperatura ambiente, como la corriente crítica, así como la resistencia a flexión se mejoran cuando la Ag se adiciona a estas muestras dopadas con Pb, mientras que no se observa cambio en la temperatura crítica. Por otra parte, se ha encontrado que las muestras de composición (Bi1.6Pb0.4Sr2CaCu2O8+δ + Ag presentan una gran pendiente de la curva E-I en la zona de transición entre el estado superconductor y el estado normal. Con estas composiciones, se han encontrado valores de n (E∼In de hasta 45 a 65K.

  13. Microwave measurements and beam dynamics simulations of the BNL/SLAC/UCLA emittance-compensated 1.6-cell photocathode rf gun

    Science.gov (United States)

    Palmer, Dennis T.; Miller, Roger H.; Winick, Herman; Wang, Xi J.; Batchelor, Kenneth; Woodle, Martin H.; Ben-Zvi, Ilan

    1995-09-01

    A dedicated low energy (2 to 10 MeV) experimental beam line is now under construction at Brookhaven National Laboratory/Accelerator Test Facility (BNL/ATF) for photocathode RF gun testing and photoemission experiments. Microwave measurements of the 1.6 cell photocathode RF gun have been conducted along with beam dynamics simulations of the emittance compensated low energy beam. These simulations indicate that the 1.6 cell photocathode RF gun in combination with solenoidal emittance compensation will be capable of producing a high brightness beam with a normalization rms emittance of (epsilon) n,rms approximately equals 1 (pi) mm mrad. The longitudinal accelerating field Ez has been measured as a function of azimuthal angle in the full cell of the cold test model for the 1.6 cell BNL/SLAC/UCLA #3 S-band RF Gun using a needle rotation/frequency perturbation technique. These measurements were conducted before and after symmetrizing the full cell with a vacuum pump out port and an adjustable short. Two different waveguide to full cell coupling schemes were studied. Experimental and theoretical studies of the field balance versus mode separation were conducted. The dipole mode of the full cell using the (theta) - coupling scheme is an order of magnitude less severe before symmetrization than the Z- coupling scheme. The multi-pole contribution to the longitudinal field asymmetry are calculated using standard Fourier series techniques for both coupling schemes. The Panofsky- Wenzel theorem is used in estimating the transverse emittance due to the multipole components of Ez. Detailed beam dynamics simulations were performed for the 1.6 cell photocathode RF gun injector using a solenoidal emittance compensation technique. The design of the experimental line along with a proposed experimental program using the 1.6 cell photocathode RF gun developed by the BNL/SLAC/UCLA RF gun collaboration is presented. This experimental program includes measurements of beam loading caused

  14. Environmental Report 1996, Volume 2

    Energy Technology Data Exchange (ETDEWEB)

    Harrach, R.J.

    1996-01-01

    This is Volume 2 of the Lawrence Livermore National Laboratory`s (LLNL`s) annual Environmental Report 1996, prepared for the US Department of Energy. Volume 2 supports Volume 1 summary data and is essentially a detailed data report that provides individual data points, where applicable. Volume 2 includes information on monitoring of air, air effluents, sewerable water, surface water, ground water, soil and sediment, vegetation and foodstuff, environmental radiation, and quality assurance.

  15. The therapeutic effect of mouse β-1, 6-glucan-specific IgG on systemic candidasis in mice%IgG型β-1,6-葡聚糖特异性抗体在治疗白色念珠菌感染中的作用

    Institute of Scientific and Technical Information of China (English)

    孙树良; 董红亮; 高晓明

    2015-01-01

    目的 探讨IgG型β-l,6-葡聚糖特异性抗体在抗白色念珠菌感染过程中的作用.方法 首先通过还原胺法将β-1,6-葡聚糖(pustulan)与载体蛋白牛血清白蛋白(BSA)偶联来免疫小鼠,以制备IgG型β-1,6-葡聚糖特异性多抗,通过流式细胞术确定该抗体能够很好地识别白色念珠菌表面的β-1,6-葡聚糖.进而,我们通过抗血清过继转移实验进一步研究了IgG型β-1,6-葡聚糖特异性多抗在抗真菌感染中的作用.结果 ①将pustulan与BSA偶联可以有效地诱导IgG型β-1,6-葡聚糖特异性多抗的产生.②流式结果显示该抗体能够很好地识别白色念珠菌表面的β-1,6-葡聚糖.③抗血清过继转移实验结果显示IgG型β-1,6-葡聚糖特异性多抗在体内可以明显减少经白色念珠菌感染的小鼠肾脏的真菌载量.④体外抑菌实验结果显示,IgG型β-1,6-葡聚糖特异性多抗在体外可以较好的抑制真菌的生长.结论 IgG型β-1,6-葡聚糖特异性抗体具有抗白色念珠菌感染的作用,这可能为真菌感染的诊断与治疗提供新的途径.

  16. REFLECTION AND REFRACTION, VOLUME 2.

    Science.gov (United States)

    KLAUS, DAVID J.; AND OTHERS

    THIS VOLUME 2 OF A TWO-VOLUME SET PROVIDES AUTOINSTRUCTION IN PHYSICS. THE UNITS COVERED IN THIS VOLUME ARE (1) REFLECTION OF LIGHT, (2) PHOTOMETRY, (3) POLARIZATION, (4) REFRACTION OF LIGHT, (5) SNELL'S LAW, (6) LENSES, FOCUS, AND FOCAL POINTS, (7) IMAGE FORMATION, AND (8) ABERRATIONS, THE EYE, AND MAGNIFICATION. THE INTRODUCTION AND UNITS ON…

  17. Calculus Students' Understanding of Volume

    Science.gov (United States)

    Dorko, Allison; Speer, Natasha M.

    2013-01-01

    Researchers have documented difficulties that elementary school students have in understanding volume. Despite its importance in higher mathematics, we know little about college students' understanding of volume. This study investigated calculus students' understanding of volume. Clinical interview transcripts and written responses to volume…

  18. The Study on preparation and purification of Fructose-1,6-Diphosphate(FDP) with brewer's yeast%利用啤酒废酵母生产1,6-二磷酸果糖发酵工艺的研究

    Institute of Scientific and Technical Information of China (English)

    李祥; 罗仓学; 李力; 张广栋

    2004-01-01

    啤酒废酵母生产1,6-二磷酸果糖发酵工艺进行了实验性探讨.正交试验结果表明:发酵液中氯化镁的浓度、pH是1,6-二磷酸果糖生产最主要的影响因素.最佳发酵参数为发酵温度37℃,葡萄糖浓度0.4mol/L,磷酸盐浓度0.4mol/L,pH6.5,有机溶剂的加量约6%.发酵液中1,6-二磷酸果糖的量最高可达70.5mg/mL.

  19. A new electric method for non-invasive continuous monitoring of stroke volume and ventricular volume-time curves

    Directory of Open Access Journals (Sweden)

    Konings Maurits K

    2012-08-01

    caused a pattern over the thorax quite distinct from that of atrial filling. The in-vivo tests of the HCP with LVot Doppler resulted in a Pearson’s correlation of R = 0.892, and Bland-Altman plotting of SV yielded a mean bias of -1.6 ml and 2SD =14.8 ml. Conclusions The results indicate that the HCP was able to track the changes in ventricular stroke volume reliably. Furthermore, the HCP produced ventricular volume-time curves that were consistent with the literature, and may be a diagnostic tool as well.

  20. Volume of an Industrial Autoclave

    Directory of Open Access Journals (Sweden)

    Nicholas Madaffari

    2010-01-01

    Full Text Available We were able to determine the volume of an industrial autoclave sterilization tank using a technique learned in calculus. By measuring the dimensions of the tank and roughly estimating the equation of curvature at the ends of the tank, we were able to revolve half of the end of the tank around the x axis to get its fluid volume. Adding the two volumes of the ends and the volume of the cylindrical portion on the tank yielded the total volume.

  1. 1,6- and 1,7-Regioisomers of Highly Soluble Amino-Substituted Perylene Tetracarboxylic Dianhydrides: Synthesis, Optical and Electrochemical Properties

    Directory of Open Access Journals (Sweden)

    Kew-Yu Chen

    2015-08-01

    Full Text Available 1,6- and 1,7-regioisomers of diamino-substituted perylene tetracarboxylic dianhydrides (PTCDs with different n-alkyl chain lengths (n = 6, 12 or 18 were synthesized and characterized by NMR spectroscopy and high-resolution mass spectrometry. These dyes are highly soluble in most organic solvents and even in nonpolar solvents, such as hexane. To the best of our knowledge, this is the first time the 1,6-diamino-substituted PTCDs (2a–2c have been obtained in pure form. The regioisomers 1a–1c (1,7- and 2a–2c (1,6- exhibit significant differences in their optical characteristics. In addition to the longest wavelength absorption band at around 674 nm, 2a–2c exhibit another shoulder band at ca. 600 nm, and consequently, cover a large part of the visible region relative to those of 1a–1c. Upon excitation, 2a–2c also show larger dipole moment changes than those of 1a–1c; the dipole moments of all compounds have been estimated using Lippert–Mataga equation. Moreover, all the dyes show a unique charge transfer emission in the near-infrared region, of which the peak wavelengths exhibit strong solvatochromism. They all exhibit one irreversible one-electron oxidation and two quasi-reversible one-electron reductions in dichloromethane at modest potentials. Complementary density functional theory calculations performed on these chromophores are reported in order to rationalize their electronic structure and optical properties.

  2. Enzymology and structure of the GH13_31 glucan 1,6-α-glucosidase that confers isomaltooligosaccharide utilization in the probiotic Lactobacillus acidophilus NCFM.

    Science.gov (United States)

    Møller, Marie S; Fredslund, Folmer; Majumder, Avishek; Nakai, Hiroyuki; Poulsen, Jens-Christian N; Lo Leggio, Leila; Svensson, Birte; Abou Hachem, Maher

    2012-08-01

    Isomaltooligosaccharides (IMO) have been suggested as promising prebiotics that stimulate the growth of probiotic bacteria. Genomes of probiotic lactobacilli from the acidophilus group, as represented by Lactobacillus acidophilus NCFM, encode α-1,6 glucosidases of the family GH13_31 (glycoside hydrolase family 13 subfamily 31) that confer degradation of IMO. These genes reside frequently within maltooligosaccharide utilization operons, which include an ATP-binding cassette transporter and α-glucan active enzymes, e.g., maltogenic amylases and maltose phosphorylases, and they also occur separated from any carbohydrate transport or catabolism genes on the genomes of some acidophilus complex members, as in L. acidophilus NCFM. Besides the isolated locus encoding a GH13_31 enzyme, the ABC transporter and another GH13 in the maltooligosaccharide operon were induced in response to IMO or maltotetraose, as determined by reverse transcription-PCR (RT-PCR) transcriptional analysis, suggesting coregulation of α-1,6- and α-1,4-glucooligosaccharide utilization loci in L. acidophilus NCFM. The L. acidophilus NCFM GH13_31 (LaGH13_31) was produced recombinantly and shown to be a glucan 1,6-α-glucosidase active on IMO and dextran and product-inhibited by glucose. The catalytic efficiency of LaGH13_31 on dextran and the dextran/panose (trisaccharide) efficiency ratio were the highest reported for this class of enzymes, suggesting higher affinity at distal substrate binding sites. The crystal structure of LaGH13_31 was determined to a resolution of 2.05 Å and revealed additional substrate contacts at the +2 subsite in LaGH13_31 compared to the GH13_31 from Streptococcus mutans (SmGH13_31), providing a possible structural rationale to the relatively high affinity for dextran. A comprehensive phylogenetic and activity motif analysis mapped IMO utilization enzymes from gut microbiota to rationalize preferential utilization of IMO by gut residents.

  3. Synthesis of 1,6-Methano [10] annulene-[3,4-g]anthraquinone Derivatives%1,6-亚甲基桥[10]轮烯并[3,4-g]-蒽醌衍生物的合成

    Institute of Scientific and Technical Information of China (English)

    屈莹; 王志华; 刘建军; 左胜利

    2015-01-01

    采用无溶剂合成法,以1,6-亚甲基桥[10]轮烯-3,4-二甲酸酐为原料,ZrOCl2.8H2O/NaCl为催化剂,于165℃下分别与取代苯经Friedel-Crafts缩合反应合成了4个新型的1,6-亚甲基桥[10]轮烯并[3,4-g]-蒽醌衍生物,其结构经1H NMR,13C NMR,IR,MS和HR-MS表征.

  4. 新型N-氨基取代的1,6-亚甲基桥[10]轮烯-3,4-二甲酰亚胺的无溶剂合成%Solvent-free Synthesis of Novel N-amino-substituted 1,6-Methano[10]annulene-3,4-dicarboximide

    Institute of Scientific and Technical Information of China (English)

    金锋; 刘建军; 陈海平; 左胜利

    2009-01-01

    1,6-亚甲基桥[10]轮烯-3,4-二乙酯为原料,简便地合成了二甲酸酐(2);2与肼在氮气保护下,无溶剂于170 ℃反应30 min,以较高收率合成了4个新型N-氨基取代的1,6-亚甲基桥[10]轮烯-3,4-二甲酰亚胺,其结构经1H NMR, 13C NMR, MS及HR-MS表征.

  5. Racemic but tropos (chirally flexible) BIPHEP ligands for Rh(I)-complexes: highly enantioselective ene-type cyclization of 1,6-enynes.

    Science.gov (United States)

    Mikami, Koichi; Kataoka, Shohei; Yusa, Yukinori; Aikawa, Kohsuke

    2004-10-14

    [reaction: see text] The tropos (chirally flexible) or atropos (chirally rigid) nature of BIPHEP-Rh complexes at room temperature critically depends on the amines complexed. The aliphatic DPEN complex is atropos, whereas the aromatic DABN complex is tropos. BIPHEP-Rh chirality can thus be controlled by DABN at room temperature. The amine-free BIPHEP-Rh complex is tropos. At 5 degrees C, even amine-free BIPHEP-Rh complexes are atropos and hence can be used as enantiopure catalysts to give high enantioselectivity in ene-type cyclization of 1,6-enynes.

  6. Oxygen isotope effect on the superconductivity and stripe phase in La$_{1.6-x}$Nd$_{0.4}$Sr$_{x}$CuO$_4$

    OpenAIRE

    Wang, G. Y.; Zhang, J D; Yang, R. L.; Chen, X. H.

    2007-01-01

    The oxygen isotope effect on the superconductivity, stripe phase and structure transition is systematically investigated in La$_{1.6-x}$Nd$_{0.4}$Sr$_{x}$CuO$_4$ with static stripe phase. Substitution of $^{16}$O by $^{18}$O leads to a decrease in superconducting transition temperature T$_C$, while enhances the temperature of the structural transition from low-temperature-orthorhombic (LTO) phase to low-temperature-tetragonal (LTT) phase. Compared to the Nd free sample, a larger isotope effec...

  7. Coexistence of, and Competition between, Superconductivity and Charge-Stripe Order in La1.6-xNd0.4SrxCuO4

    Science.gov (United States)

    Tranquada, J. M.; Axe, J. D.; Ichikawa, N.; Moodenbaugh, A. R.; Nakamura, Y.; Uchida, S.

    1997-01-01

    Previously we have presented evidence for stripe order of holes and spins in La1.6-xNd0.4SrxCuO4 with x = 0.12. Here we show, via neutron diffraction measurements of magnetic scattering, that similar order occurs in crystals with x = 0.15 and 0.20. Zero-field-cooled magnetization measurements show that all three compositions are also superconducting, with the superconducting transition temperature increasing as the low-temperature staggered magnetization decreases. These results directly demonstrate an intimate connection between stripe correlations and superconductivity.

  8. Dosimetric characteristics of a radiochromic polyvinyl butyral film containing 2,4-hexadiyn-1,6-bis(n-butyl urethane).

    Science.gov (United States)

    Abdel-Fattah, A A; Soliman, Y S; Bayomi, A M M; Abdel-Khalek, A A

    2014-04-01

    A radiation-sensitive compound 2,4-hexadiyn-1,6-bis(n-butyl urethane) (HDDBU) was synthesized, characterized by FTIR spectroscopy, and introduced into a thin polyvinyl butyral film to form a radiation dosimeter for industrial irradiation facilities. The monomer polymerizes under gamma radiation, inducing change in the film spectrum in the range of 200-400 nm. According to XRD spectroscopy, the film contains monomeric HDDBU in a non-crystalline state. The dose response function, radiation sensitivity, and dependences of the response on environmental factors were studied. Uncertainty of dose measurements with the proposed dosimetry system was analyzed in detail.

  9. Selective Oxidation of 1,6-Hexanediol to 6-Hydroxycaproic Acid over Reusable Hydrotalcite-Supported Au-Pd Bimetallic Catalysts.

    Science.gov (United States)

    Tuteja, Jaya; Nishimura, Shun; Choudhary, Hemant; Ebitani, Kohki

    2015-06-08

    Selective oxidation of 1,6-hexanediol into 6-hydroxycaproic acid was achieved over hydrotalcite-supported Au-Pd bimetallic nanoparticles as heterogeneous catalyst using aqueous H2 O2 . N,N-dimethyldodecylamine N-oxide (DDAO) was used as an efficient capping agent. Spectroscopic analyses by UV/Vis, TEM, XPS, and X-ray absorption spectroscopy suggested that interactions between gold and palladium atoms are responsible for the high activity of the reusable Au40 Pd60 -DDAO/HT catalyst.

  10. PCR-SSP在人类血小板抗原1-6、15系统基因分型中的应用

    Institute of Scientific and Technical Information of China (English)

    周琼秀; 李执如; 陈静娴

    2009-01-01

    目的 探索PCR-SSP技术在人类血小板抗原(HPA)-1、2、3、4、5、6、15系统的基因分型中的应用.方法 合成23条序列特异性引物,通过调节引物浓度、Mg2+离子浓度和探索最佳PCR扩增条件,建立HPA-1-6、15系统同步扩增基因分型方法.用该法盲检100名献血者HPA-1-6、15系统的基因分型结果,与用美国G&T1??3/4μ?HPA-1-6、15系统的基因分型试剂所测试的这100名献血者的基因分型结果进行比较.结果 该分型方法和美国G&T1??3/4ê?1/4áí?ê±1/4ì2a100名随机献血者,其HPA-1-6、15分型结果完全一致,符合率达100%,基因频率分别是:HPA-1a和1b为1和0,HPA-2a和2b为0.98和0.02,HPA-3a和3b为0.63和0.37,HPA-4a和4b为0.997和0.003, HPA-5a和5b为0.997和0.003, HPA-6a和6b为1和0,HPA-15a和15b为0.548和0.450.结论 该分型方法具有简便、快速、准确的特点, 适合常规HPA基因分型.

  11. Expression of sodium channel α subunits 1.1, 1.2 and 1.6 in rat hippocampus after kainic acid-induced epilepsy.

    Science.gov (United States)

    Qiao, Xin; Werkman, Taco R; Gorter, Jan A; Wadman, Wytse J; van Vliet, Erwin A

    2013-09-01

    Voltage-gated Na(+) channels control neuronal excitability and are the primary target for the majority of anti-epileptic drugs. This study investigates the (sub)cellular expression patterns of three important brain-associated Na(+) channel α subunits: NaV1.1, NaV1.2 and NaV1.6 during epileptogenesis (induced by kainic acid) using time points that cover the period from induction to the chronic phase of epilepsy. NaV1.1 immunoreactivity was persistently reduced at 1 day, 3 weeks and 2 months after SE in CA1 and CA3. About 50% of the NaV1.1-positive interneurons was lost at one day after SE in all regions investigated. In the hilus a similar reduction in NeuN-positive neurons was found, while in the CA1 and CA3 region the loss in NeuN-positive neurons only reached 15% in the chronic phase of epilepsy. This implies a stronger shift in the balance between excitation and inhibition toward excitation in the CA1 and CA3 region than in the hilus. NaV1.2 immunoreactivity in the inner molecular layer of the dentate gyrus was lower than control at 1 day after SE. It increased at 3 weeks and 2 months after SE in the inner molecular layer and overlapped with sprouted mossy fibers. NaV1.6 immunoreactivity in the dendritic region of CA1 and CA3 was persistently reduced at all time-points during epileptogenesis. Some astrocytes expressed NaV1.1 and NaV1.6 at 3 weeks after SE. Expression data alone are not sufficient to explain changes in network stability, or infer causality in epileptogenesis. These results demonstrate that hippocampal sub-regional expression of NaV1.1, NaV1.2 and NaV1.6 Na(+) channel α subunits is altered during epileptogenesis in a time and location specific way. This implies that understanding epileptogenesis has to take into account several distinct and type-specific changes in sodium channel expression.

  12. Hydroformylation of olefins and reductive carbonylation of aryl halides with syngas formed ex situ from dehydrogenative decarbonylation of hexane-1,6-diol

    DEFF Research Database (Denmark)

    Christensen, Stig Holden; Olsen, Esben Paul Krogh; Rosenbaum, Jascha;

    2014-01-01

    A variety of primary alcohols have been investigated as convenient substrates for the ex situ delivery of carbon monoxide and molecular hydrogen in a two-chamber reactor. The gaseous mixture is liberated in one chamber by an iridium-catalysed dehydrogenative decarbonylation of the alcohol...... and then consumed in the other chamber in either a rhodium-catalysed hydroformylation of olefins or a palladium-catalysed reductive carbonylation of aryl halides. Hexane-1,6-diol was found to be the optimum alcohol for both reactions where moderate to excellent yields were obtained of the product aldehydes...

  13. α,β-Unsaturated Gold(I) Carbenes by Tandem Cyclization and 1,5-Alkoxy Migration of 1,6-Enynes: Mechanisms and Applications.

    Science.gov (United States)

    Calleja, Pilar; Pablo, Óscar; Ranieri, Beatrice; Gaydou, Morgane; Pitaval, Anthony; Moreno, María; Raducan, Mihai; Echavarren, Antonio M

    2016-09-12

    1,6-Enynes bearing OR groups at the propargyl position generate α,β-unsaturated gold(I)-carbenes/ gold(I) stabilized allyl cations that can be trapped by alkenes to form cyclopropanes or 1,3-diketones to give products of α-alkylation. The best migrating group is p-nitrophenyl ether, which leads to the corresponding products without racemization. Thus, an improved formal synthesis of (+)-schisanwilsonene A has been accomplished. The different competitive reaction pathways have been delineated computationally.

  14. Light Propagation Volumes

    OpenAIRE

    Mikulica, Tomáš

    2016-01-01

    Cílem diplomové práce je popsat různé metody výpočtu globálního osvětlení scény včetně techniky Light Propagation Volumes. Pro tuto metodu jsou podrobně popsány všechny tři kroky výpočtu: injekce, propagace a vykreslení. Dále je navrženo několik vlastních rozšíření zlepšující grafickou kvalitu metody. Části návrhu a implementace jsou zaměřeny na popis scény, zobrazovacího systému, tvorby stínů, implementace metody Light Propagation Volumes a navržených rozšíření. Práci uzavírá měření, porovná...

  15. RadioAstron space VLBI imaging of polarized radio emission in the high-redshift quasar 0642+449 at 1.6 GHz

    CERN Document Server

    Lobanov, A P; Bruni, G; Kovalev, Y Y; Anderson, J; Bach, U; Kraus, A; Zensus, J A; Lisakov, M M; Sokolovsky, K V; Voytsik, P A

    2015-01-01

    Polarization of radio emission in extragalactic jets at a sub-milliarcsecond angular resolution holds important clues for understanding the structure of the magnetic field in the inner regions of the jets and in close vicinity of the supermassive black holes in the centers of active galaxies. Space VLBI observations provide a unique tool for polarimetric imaging at a sub-milliarcsecond angular resolution and studying the properties of magnetic field in active galactic nuclei on scales of less than 10^4 gravitational radii. A space VLBI observation of high-redshift quasar TXS 0642+449 (OH 471), made at a wavelength of 18 cm (frequency of 1.6 GHz) as part of the Early Science Programme (ESP) of the RadioAstron} mission, is used here to test the polarimetric performance of the orbiting Space Radio Telescope (SRT) employed by the mission, to establish a methodology for making full Stokes polarimetry with space VLBI at 1.6 GHz, and to study the polarized emission in the target object on sub-milliarcsecond scales. ...

  16. Experimental Demonstration of 84 Gb/s PAM-4 Over up to 1.6 km SSMF Using a 20-GHz VCSEL at 1525 nm

    DEFF Research Database (Denmark)

    Eiselt, Nicklas; Griesser, Helmut; Wei, Jinlong

    2017-01-01

    for a transmission distance of >0.6 km, while the use of an NLVE or FFE + MLSE is able to improve the transmission distance towards 1 km. The use of partial-response PAM-4 FFE in combination with a short memory MLSE is able to efficiently equalize the bandwidth limitations, showing more than 10-times BER improvement...... feedforward equalizer, a nonlinear Volterra equalizer (NLVE), a maximum likelihood sequence estimator (MLSE) and their combinations are evaluated working either as an equalizer for a standard PAM-4 or a partial response PAM-4 signal with seven levels. It is demonstrated that a standard FFE is not enough...... compared to standard NLVE or FFE + MLSE at a transmission distance of 1.6 km. Using a partial-response NLVE instead of an PR-FFE further performance improvement is achieved, resulting in BERs below the KP4 FEC-threshold with a BER-limit of 2E-4 after 1.6-km transmission distance, allowing error free...

  17. A phase I/II trial of beta-(1,3/(1,6 D-glucan in the treatment of patients with advanced malignancies receiving chemotherapy

    Directory of Open Access Journals (Sweden)

    Weitberg Alan B

    2008-09-01

    Full Text Available Abstract β-(1,3/(1,6 D-glucan, a component of the fungal cell wall, has been shown to stimulate the immune system, enhance hematopoiesis, amplify killing of opsonized tumor cells and increase neutrophil chemotaxis and adhesion. In view of these attributes, the β-glucans should be studied for both their therapeutic efficacy in patients with cancer as well as an adjunctive therapy in patients receiving chemotherapy as a maneuver to limit suppression of hematopoiesis. In this study, twenty patients with advanced malignancies receiving chemotherapy were given a β-(1,3/(1,6 D-glucan preparation (MacroForce plus IP6, ImmuDyne, Inc. and monitored for tolerability and effect on hematopoiesis. Our results lead us to conclude that β-glucan is well-tolerated in cancer patients receiving chemotherapy, may have a beneficial effect on hematopoiesis in these patients and should be studied further, especially in patients with chronic lymphocytic leukemia and lymphoma.

  18. Profiling of core fucosylated N-glycans using a novel bacterial lectin that specifically recognizes α1,6 fucosylated chitobiose

    Science.gov (United States)

    Vainauskas, Saulius; Duke, Rebecca M.; McFarland, James; McClung, Colleen; Ruse, Cristian; Taron, Christopher H.

    2016-01-01

    A novel fucose-binding lectin (SL2-1) from the bacterium Streptomyces rapamycinicus was identified by analysis of metagenomic DNA sequences. SL2-1 belongs to a new group of bacterial fucose-specific lectins that have no similarity to known bacterial fucose-binding proteins, but are related to certain eukaryotic fucose-binding lectins. The 17 kDa protein was expressed recombinantly in E. coli and purified by affinity chromatography. Glycan microarray analysis with fluorescently labeled recombinant SL2-1 demonstrated its ability to bind to core α1-6 fucosylated N-glycans, but not to core α1-3 fucosylated N-glycans, or other α1-2, α1-3 and α1-4 fucosylated oligosaccharides. The minimal high affinity binding epitope of SL2-1 was α1-6 fucosylated di-n-acetylchitobiose. The recombinant lectin was efficient in detection of N-glycan core fucosylation using lectin blotting and lectin ELISA assays. Finally, a workflow using SL2-1 for selective and quantitative profiling of core fucosylated N-glycans using UPLC-HILIC-FLR analysis was established. The approach was validated for selective capture and analysis of core fucosylated N-glycans present in complex glycan mixtures derived from mammalian serum IgG. PMID:27678371

  19. Structures of incommensurate and commensurate composite crystals NaxCuO2 (x=1.58, 1.6, 1.62).

    Science.gov (United States)

    van Smaalen, Sander; Dinnebier, Robert; Sofin, Mikhail; Jansen, Martin

    2007-02-01

    NaxCuO2 (x approximately 1.6) has been synthesized for different compositions x, resulting in both commensurate and incommensurate composite crystals. The crystal structures are reported for two incommensurate compounds (x=1.58 and 1.62) determined by Rietveld refinements against X-ray powder diffraction data. The incommensurate compounds and commensurate Na8Cu5O10 (x=1.6) are found to possess similar structures, with valence fluctuations of Cu2+/Cu3+ as the origin of the modulations of the CuO2 subsystems; the displacive modulations of Na being defined by the closest Na-O contacts between the subsystems. A comparison of the structure models obtained from single-crystal X-ray diffraction, synchrotron-radiation X-ray powder diffraction and X-ray powder diffraction with Cu Kalpha1 radiation indicates that single-crystal X-ray diffraction is by far the most accurate method, while powder diffraction with radiation from an X-ray tube provides the least accurate structure model.

  20. Structures of incommensurate and commensurate composite crystals Na{sub x}CuO{sub 2} (x=1.58,1.6,1.62)

    Energy Technology Data Exchange (ETDEWEB)

    Smaalen, S. van [Bayreuth Univ. (Germany). Lab. of Crystallography; Dinnebier, R.; Sofin, M.; Jansen, M. [Max-Planck-Institute for Solid State Research, Stuttgart (Germany)

    2007-02-15

    Na{sub x}CuO{sub 2} (x{approx_equal}1.6) has been synthesized for different compositions x, resulting in both commensurate and incommensurate composite crystals. The crystal structures are reported for two incommensurate compounds (x=1.58 and 1.62) determined by Rietveld refinements against X-ray powder diffraction data. The incommensurate compounds and commensurate Na{sub 8}Cu{sub 5}O{sub 10} (x=1.6) are found to possess similar structures, with valence fluctuations of Cu{sup 2+}/Cu{sup 3+} as the origin of the modulations of the CuO{sub 2} subsystems; the displacive modulations of Na being defined by the closest Na-O contacts between the subsystems. A comparison of the structure models obtained from single-crystal X-ray diffraction, synchrotron-radiation X-ray powder diffraction and Xray powder diffraction with Cu K{alpha}{sub 1} radiation indicates that single-crystal X-ray diffraction is by far the most accurate method, while powder diffraction with radiation from an Xray tube provides the least accurate structure model. (orig.)

  1. Structures of Incommensurate and Commensurate Composite Crystals NaxCuO2(x=1.58, 1.6, 1.62)

    Energy Technology Data Exchange (ETDEWEB)

    van Smaalen,S.; Dinnebier, R.; Sofin, M.; Jansen, M.

    2007-01-01

    NaxCuO2 (x {approx_equal} 1.6) has been synthesized for different compositions x, resulting in both commensurate and incommensurate composite crystals. The crystal structures are reported for two incommensurate compounds (x = 1.58 and 1.62) determined by Rietveld refinements against X-ray powder diffraction data. The incommensurate compounds and commensurate Na{sub 8}Cu{sub 5}O{sub 10} (x = 1.6) are found to possess similar structures, with valence fluctuations of Cu{sup 2+}/Cu{sup 3+} as the origin of the modulations of the CuO{sub 2} subsystems; the displacive modulations of Na being defined by the closest Na-O contacts between the subsystems. A comparison of the structure models obtained from single-crystal X-ray diffraction, synchrotron-radiation X-ray powder diffraction and X-ray powder diffraction with Cu K{sub 1} radiation indicates that single-crystal X-ray diffraction is by far the most accurate method, while powder diffraction with radiation from an X-ray tube provides the least accurate structure model.

  2. Effects of grain size on high temperature creep of fine grained, solution and dispersion hardened V-1.6Y-8W-0.8TiC

    Energy Technology Data Exchange (ETDEWEB)

    Furuno, T. [Ehime Univerisity, 3 Bunkyo-cho, Matsuyama 790-8577 (Japan); Kurishita, H., E-mail: kurishi@imr.tohoku.ac.jp [International Research Center for Nuclear Materials Science, Institute for Materials Research (IMR), Tohoku University, Oarai, Ibaraki 311-1313 (Japan); Nagasaka, T.; Nishimura, A.; Muroga, T. [Fusion Engineering Research Center, National Institute for Fusion Science (NIFS), Oroshi-cho 322-6, Tok, Gifu 292 (Japan); Sakamoto, T.; Kobayashi, S.; Nakai, K. [Department of Materials Science and Biotechnology, Ehime Univerisity, 3 Bunkyo-cho, Matsuyama 790-8577 (Japan); Matsuo, S.; Arakawa, H. [International Research Center for Nuclear Materials Science, Institute for Materials Research (IMR), Tohoku University, Oarai, Ibaraki 311-1313 (Japan)

    2011-10-01

    Creep resistance is the major concern of vanadium and its alloys for fusion reactor structural applications. In order to elucidate the effects of grain size on the creep behavior of solution and dispersion strengthened vanadium alloys, V-1.6Y-8W-0.8TiC specimens with fine grain sizes from 0.58 to 1.45 {mu}m were prepared by mechanical alloying and HIP without any plastic working and tested at 1073 K and 250 MPa in vacuum. It is shown that the creep resistance of V-1.6Y-8W-0.8TiC depends strongly on grain size and increases with increasing grain size: The creep life for the grain size of 1.45 {mu}m is almost one order longer than that of 0.58 {mu}m, and about two orders longer than that of V-4Cr-4Ti (NIFS-Heat 2) although the grain size of V-4Cr-4Ti is as large as 17.8 {mu}m. The observed creep behavior is discussed in terms of grain size effects on dislocation glide and grain boundary sliding.

  3. Influence of line mixing on the retrievals of atmospheric CO2 from spectra in the 1.6 and 2.1 μm regions

    Directory of Open Access Journals (Sweden)

    G. C. Toon

    2009-02-01

    Full Text Available We present the first study of the influence of line mixing among CO2 lines on the remote sensing retrieval of atmospheric carbon dioxide. This is done in the bands near 1.6 and 2.1 μm which have been retained by the Orbiting Carbon Observatory (OCO and Greenhouse Gases Observatory Satellite (GOSAT instruments. A purely theoretical analysis is first made, based on simulations of atmospheric spectra. It shows that line mixing cannot be neglected since disregarding this process induces significant errors in the calculated absorption coefficients, leading to systematic structures in the spectral fit residuals and airmass-dependent biases in the retrieved CO2 amounts. These theoretical predictions are then confirmed by using atmospheric solar-absorption spectra measured by a ground-based Fourier transform spectrometer. Indeed, it is first shown that including line mixing in the forward model used for the inversion leads to a very significant reduction of the residuals in the 2.1 μm region. Secondly, the inclusion of line mixing reduces the dependence of the retrieved CO2 on the airmass and greatly improves the consistency between values obtained independently from spectra in the 1.6 and 2.1 μm bands. These results open very promising prospects for various ground-based and space-borne experiments monitoring the carbon dioxide atmospheric amounts.

  4. Cloning of a NaCl-induced fructose-1, 6-diphosphate aldolase cDNA from Dunaliella salina and its expression in tobacco

    Institute of Scientific and Technical Information of China (English)

    ZHANG; Xiaoning; (张晓宁); LIN; Changfa; (林长发); CHEN; Huoying; (陈火英); WANG; Hao; (王; 昊); QU; Zhicai; (曲志才); ZHANG; Hongwei; (张宏伟); YAO; Jianhong; (姚剑虹); SHEN; Daleng; (沈大棱)

    2003-01-01

    Using Rapid Amplification of cDNA ends (RACE) technique, the full-length cDNA encoding a NaCl-induced fructose-1, 6- diphosphate aldolase (DsALDP) was obtained. It was shown that the DsALDP had a relatively high homology (66%-73%) to chloroplast fructose-1, 6-diphos- phate aldolase (AldP) in many plants according to their amino acid sequences. The phylogenetic analysis further confirmed that AldP in alga is the nearest to DsALDP. As to its expression pattern, DsALDP was de novo synthesized by NaCl induction. Its expression level was significantly changed with inducing time. After the selected DsALDP cDNA subcloned into a binary vector pBI121, the new construct was introduced into tobacco by Agrobacterium tumefaciens. The results of Southern blot and RT-PCR analysis of four transgenic T1 plants indicated that DsALDP was integrated into genome of these transgenic plants and effectively expressed. Aldolase activities have been detected in T1-1, T1-2 and T1-3 plants by bioassay under 100-200 mmol/L NaCl. It was also observed that proline contents in them were differentially increased.

  5. Extraction, characterization and biological activity of a (1,3)(1,6)-β-d-glucan from the pathogenic oomycete Pythium insidiosum.

    Science.gov (United States)

    Tondolo, Juliana S M; Ledur, Pauline C; Loreto, Érico S; Verdi, Camila M; Bitencourt, Paula E R; de Jesus, Francielli P K; Rocha, Jean P; Alves, Sydney H; Sassaki, Guilherme L; Santurio, Janio M

    2017-02-10

    Pythiosis is a life-threatening infectious disease caused by the pathogenic oomycete Pythium insidiosum. This study is the first to evaluate the P. insidiosum glucan content and its biological activities. The enzymatic quantification of the glucans in P. insidiosum mycelia showed that the β-glucan content was 18.99%±3.59. The cell wall polysaccharide extract consisted of ∼81.7% carbohydrates (exclusively glucose) and ∼18.3% residual amino acids and peptides. The results from monosaccharide composition, methylation and 1D/2D NMR spectroscopy analyses indicated the presence of a highly branched (1,3)(1,6)-β-d-glucan, with (1,6)-β-d-glucopyranosil side-branching unit on average every 1-2 repeat units. In vitro, the β-d-glucan extract could significantly promote spleen lymphocyte proliferation in human, equine and mouse cell cultures. BALB/c mice that were subcutaneously pre-immunized with three doses of 0.5, 2.5 and 5.0mg of β-glucan/mouse, showed a significant increase in IL-2, IL-6, IL-10, TNF-α and IL-17A production compared to non-immunized mice. These results suggested that β-d-glucan extract induces significant and specific Th17 cellular immune response and provided the theoretical basis for further experiments. Copyright © 2016 Elsevier Ltd. All rights reserved.

  6. Differential gene expression in Xylella fastidiosa 9a5c during co-cultivation with the endophytic bacterium Methylobacterium mesophilicum SR1.6/6.

    Science.gov (United States)

    Dourado, Manuella Nóbrega; Santos, Daiene Souza; Nunes, Luiz Roberto; Costa de Oliveira, Regina Lúcia Batista da; de Oliveira, Marcus Vinicius; Araújo, Welington Luiz

    2015-12-01

    Xylella fastidiosa, the causal agent of citrus variegated chlorosis (CVC), colonizes plant xylem, reducing sap flow, and inducing internerval chlorosis, leaf size reduction, necrosis, and harder and smaller fruits. This bacterium may be transmitted from plant to plant by sharpshooter insects, including Bucephalogonia xanthopis. The citrus endophytic bacterium Methylobacterium mesophilicum SR1.6/6 colonizes citrus xylem and previous studies showed that this strain is also transferred from plant to plant by B. xanthopis (Insecta), suggesting that this endophytic bacterium may interact with X. fastidiosa in planta and inside the insect vector during co-transmission by the same insect vector. To better understand the X. fastidiosa behavior in the presence of M. mesophilicum, we evaluated the X. fastidiosa transcriptional profile during in vitro interaction with M. mesophilicum SR1.6/6. The results showed that during co-cultivation, X. fastidiosa down-regulated genes related to growth and up-regulated genes related to energy production, stress, transport, and motility, suggesting the existence of a specific adaptive response to the presence of M. mesophilicum in the culture medium.

  7. Challenge pools of hepatitis C virus genotypes 1-6 prototype strains: replication fitness and pathogenicity in chimpanzees and human liver-chimeric mouse models

    DEFF Research Database (Denmark)

    Bukh, Jens; Meuleman, Philip; Tellier, Raymond

    2010-01-01

    Chimpanzees represent the only animal model for studies of the natural history of hepatitis C virus (HCV). To generate virus stocks of important HCV variants, we infected chimpanzees with HCV strains of genotypes 1-6 and determined the infectivity titer of acute-phase plasma pools in additional a...... resource for studies of HCV molecular virology and for studies of pathogenesis, protective immunity, and vaccine efficacy in vivo....... animals. The courses of first- and second-passage infections were similar, with early appearance of viremia, HCV RNA titers of >10(4.7) IU/mL, and development of acute hepatitis; the chronicity rate was 56%. The challenge pools had titers of 10(3)-10(5) chimpanzee infectious doses/mL. Human liver......-chimeric mice developed high-titer infections after inoculation with the challenge viruses of genotypes 1-6. Inoculation studies with different doses of the genotype 1b pool suggested that a relatively high virus dose is required to consistently infect chimeric mice. The challenge pools represent a unique...

  8. Religious Young Adults Recounting the Past: Narrating Sexual and Religious Cultures in School

    Science.gov (United States)

    Page, Sarah-Jane; Yip, Andrew Kam-Tuck

    2012-01-01

    Schooling can be a pivotal time in young people's formative experience when identities are negotiated and forged. However, contradictory dominant cultures can operate within the school context, making it very challenging for individuals to negotiate their religious and sexual identities within a sexualised and heteronormative space. This essay…

  9. Recounting the K-12 School Experiences of Adults Who Stutter: A Qualitative Analysis

    Science.gov (United States)

    Daniels, Derek E.; Gabel, Rodney M.; Hughes, Stephanie

    2012-01-01

    This study qualitatively explored the primary and secondary (K-12) school experiences of adults who stutter. The primary investigator conducted semi-structured interviews with 11 participants, a first focus group interview with 6 participants, and a second focus group interview with 4 participants. Participants discussed the various ways in which…

  10. Re-counting 'illiteracy': literacy skills in the sociology of social inequality.

    Science.gov (United States)

    Payne, Geoff

    2006-06-01

    Recent media reports have repeated claims that there are very large numbers of illiterate people in Britain, seven million adults being the most common figure. The moral panic this engendered has helped to justify educational initiatives and greater public spending on basic skills. However, a closer investigation of definitions and measurements of illiteracy in major national studies reveals the peculiarity of this social construction of illiteracy. Not least, it is argued that the numbers actually suffering significant literacy deficits are much lower than reported. Partly because British sociology has given literacy little attention (despite the advent of the 'New Literacy Studies'), official accounts of illiteracy remain unduly 'normative', seriously under-estimating cultural differences like gender, ethnicity and class. A new classification of low literacy skills is proposed, with the intention of demonstrating connections with structural accounts of social inequality and sociological theory. Low levels of literacy skill remain important because they are related to other forms of social inequality, even when the number of people disadvantaged in this way is much smaller than previously claimed.

  11. MY OLD-STYLE MARRIAGE Newsman Recounts Years of Unexpected Contentment

    Institute of Scientific and Technical Information of China (English)

    1994-01-01

    IT is 45 years since Chen Xiuhe and I married on January 31, 1948, when my hometown was liberated. However, at that time there existed no law about marriage, so mine was an old-style union. My mother was ill and our domestic affairs needed managing. My parents and hers decided for us that we should be married—I was 15 and she was 18. I didn’t know much about worldly affairs except I knew I should study very

  12. Communication styles of persons with ALS as recounted by surviving partners.

    Science.gov (United States)

    McKelvey, Miechelle; Evans, David L; Kawai, Norimune; Beukelman, David

    2012-12-01

    The purpose of this study was to describe the communication patterns of individuals with Amyotrophic Lateral Sclerosis (ALS) as reported from the surviving spouses' perspectives. Six surviving spouses of people with ALS participated in semi-structured interviews after the deaths of their partners. The interviews were transcribed and coded. Data analysis revealed four primary themes: communication styles, augmentative and alternative communication (AAC) use, decision-making, and lifestyle changes. Spouses described unique changes in communication styles as their partners began to use augmentative communication strategies and devices. AAC devices were described as essential for individuals with ALS with respect to decision making and maintaining social roles. Spouses stated the importance of adapting social activities in order to maintain involvement in the community as well as with family and friends.

  13. New Book Recounts Exciting, Colorful History Of Radio Astronomy in Green Bank, West Virginia

    Science.gov (United States)

    2007-07-01

    A new book published by the National Radio Astronomy Observatory (NRAO) tells the story of the founding and early years of the Observatory at Green Bank, West Virginia. But it was Fun: the first forty years of radio astronomy at Green Bank, is not a formal history, but rather a scrapbook of early memos, recollections, anecdotes and reports. But it was Fun... is liberally illustrated with archival photographs. It includes historical and scientific papers from symposia held in 1987 and 1995 to celebrate the birthdays of two of the radio telescopes at the Observatory. Book cover The National Radio Astronomy Observatory was formed in 1956 after the National Science Foundation decided to establish an observatory in the eastern United States for the study of faint radio signals from distant objects in the Universe. But it was Fun... reprints early memos from the group of scientists who searched the mountains for a suitable site -- an area free from radio transmitters and other sources of radio interference -- "in a valley surrounded by as many ranges of high mountains in as many directions as possible," which was "at least 50 miles distant from any city or other concentration of people." The committee settled on Green Bank, a small village in West Virginia, and the book documents the struggles that followed to create a world-class scientific facility in an isolated area more accustomed to cows than computers. Groundbreaking at the Observatory, then a patchwork of farms and fields, took place in October 1957, only a few days after the launch of Sputnik by the Soviet Union. A year later, Green Bank's first telescope was dedicated, and the book contains a transcription of speeches given at that ceremony, when the Cold War, the space race and America's scientific stature were issues of the hour. The centerpiece of the new Observatory was to be a highly-precise radio telescope 140 feet in diameter, but it was expected that it would soon be surpassed by dishes of much greater size. The book reprints internal memos, reports, and recollections of astronomers who were there, as the initial elation turned to frustration when the 140 Foot Telescope project became mired in technical difficulties, plans for larger dishes were put on hold, and the scientific staff of the fledgling Observatory struggled to create a National Observatory with inadequate equipment in a very remote location. Articles by David Heeschen and John Findlay tell the story of the creation of the 300 Foot Telescope, at that time the largest in the world, which went from initial concept to full operation in only 23 months, and began a rich life of research that put the NRAO on the world scientific map. The 300 Foot Telescope was originally intended to be an interim instrument, but as documented in the book, demand for its use was so high that it was kept in operation long after its initial planned retirement, with regular upgrades and new generations of electronics. The sudden collapse of the 300 Foot Telescope on a calm evening after 26 years of operation shocked the astronomical community. But it was Fun... features dramatic first-hand accounts by the people who were there that night: the telescope operator who found himself under a falling structure; the Observatory staff who at first could not believe what happened, and those who worked during the night and into the next day to secure the area, preserve information on what happened, and deal with the rush of publicity. The book includes extensive photographs and the Executive Summary Report of the panel which was commissioned to investigate the collapse and its implication for the design of other large radio telescopes. But it was Fun... will appeal to a variety of audiences. Historians of science will be interested in the articles by David Heeschen, Gerald Tape, and Hugh van Horn, on the evolution of the concept of a National Observatory, and the difficulties of putting the concepts into practice in Green Bank. Those interested in astronomical discovery will find fascinating and highly personal accounts by Peter Mez

  14. Friction and wear behavior of the superconducting compound oxide of Ag/Bi_(1. 6) Pb_(0. 4)Sr_2Ca_2Cu_3O_(10-x)%超导Ag/Bi_(1.6)Pb_(0.4)Sr_2Ca_2Cu_3O_(10-x)复合氧化物摩擦磨损性能

    Institute of Scientific and Technical Information of China (English)

    彭义; 李长生; 董丽荣; 丁巧党; 王昊璐

    2009-01-01

    The superconducting Bi_(1.6)Pb_(0.4)Sr_2Ca_2Cu_3O_(10-x) samples were prepared by the solid-state synthesismethod. The tribological properties were measured on the friction and wear tester from ambient temperature toliquid nitrogen temperature. The results indicate that the friction coefficient of Bi_(1.6)Pb_(0.4)Srz_2Ca_2Cu_30_(10-x) againststainless steel is about 0.35 at the ambient temperature and decreases abruptly to half of the normal state value whenthe temperature is below the superconducting transition temperature of Bi_(1.6)Pb_(0. 4_Srz_2Ca_2Cu_30_(10-x). Themeasurements prove directly the effect of electronic excitation on friction. Moreover, the Ag/Bi_(1.6) Pb_(0.4)Sr_2Ca_2Cu_3O_(10-x) composite samples were prepared by sintering Bi_(1.6)Pb_(0.4)Sr_2Ca_2Cu_3O_(10-x) with Ag at different mass fractions to improve the tribological properties of Bi_(1.6)Pb_(0.4)Sr_2Ca_2Cu_3O_(10-x) at room temperature. The experimental results show that the friction coefficient of 10 Wt%Ag/Bi_(1. 6) Pb_(0. 4)Sr_2Ca_2Cu_3O_(10-x) is about 0. 2~O. 3 and the wear rate is 4. 57×10~(-4)mm~3·(N·m)~(-1), and the superconductivity of 10 Wt% Ag/Bi_(1. 6) Pb_(0. 4)Sr_2Ca_2Cu_3O_(10-x) is not degenerated.%为适应20℃~-200℃温度的适用范围, 采用固相法制备了Bi_(1.6) Pb_(0. 4)Sr_2Ca_2Cu_3O_(10-x)超导材料, 用摩擦磨损试验机测试了Bi_(1.6) Pb_(0. 4)Sr_2Ca_2Cu_3O_(10-x)从液氮温度至室温的摩擦学性能. 结果表明: 在室温20℃下, Bi_(1. 6) Pb_(0. 4)Sr_2Ca_2Cu_3O_(10-x)与对偶件轴承钢盘对摩时, 摩擦系数约为0. 35, 当温度降到超导转变温度以下时(液氮温度-170℃)摩擦系数大幅度降低, Bi_(1. 6) Pb_(0. 4)Sr_2Ca_2Cu_3O_(10-x)超导态摩擦系数为正常态值的一半, 实验证明了电子激励对摩擦能量耗散的作用. 为改善室温下Bi_(1. 6) Pb_(0. 4)Sr_2Ca_2Cu_3O_(10-x)摩擦学性能, 掺杂不同质量分数Ag作为润滑组元, 制备了Ag/Bi_(1. 6) Pb_(0. 4)Sr_2Ca_2Cu_3O_(10-x)

  15. CuAAC click chemistry with N-propargyl 1,5-dideoxy-1,5-imino-D-gulitol and N-propargyl 1,6-dideoxy-1,6-imino-D-mannitol provides access to triazole-linked piperidine and azepane pseudo-disaccharide iminosugars displaying glycosidase inhibitory properties.

    Science.gov (United States)

    Zamoner, Luís Otávio B; Aragão-Leoneti, Valquíria; Mantoani, Susimaire P; Rugen, Michael D; Nepogodiev, Sergey A; Field, Robert A; Carvalho, Ivone

    2016-06-24

    Protecting group-free synthesis of 1,2:5,6-di-anhydro-D-mannitol, followed by ring opening with propargylamine and subsequent ring closure produced a separable mix of piperidine N-propargyl 1,5-dideoxy-1,5-imino-D-gulitol and azepane N-propargyl 1,6-dideoxy-1,6-imino-D-mannitol. In O-acetylated form, these two building blocks were subjected to CuAAC click chemistry with a panel of three differently azide-substituted glucose building blocks, producing iminosugar pseudo-disaccharides in good yield. The overall panel of eight compounds, plus 1-deoxynojirimycin (DNJ) as a benchmark, was evaluated as prospective inhibitors of almond β-glucosidase, yeast α-glucosidase and barley β-amylase. The iminosugar pseudo-disaccharides showed no inhibitory activity against almond β-glucosidase, while the parent N-propargyl 1,5-dideoxy-1,5-imino-D-gulitol and N-propargyl 1,6-dideoxy-1,6-imino-D-mannitol likewise proved to be inactive against yeast α-glucosidase. Inhibitory activity could be reinstated in the former series by appropriate substitution on nitrogen. The greater activity of the piperidine could be rationalized based on docking studies. Further, potent inhibition of β-amylase was observed with compounds from both the piperidine and azepane series.

  16. 喷射成形1.8C-1.6Al超高碳钢快速凝固组织研究%Microstructure of Rapid Solidification of Spray Formed 1.8C-1.6Al Ultra-High Carbon Steel

    Institute of Scientific and Technical Information of China (English)

    王军; 徐政; 史海生; 林一坚; 章靖国

    2003-01-01

    铸造1 8C-1.6Al超高碳钢(UHCS)由于冷速较低,晶粒粗大,珠光体片间距也较大,晶界形成了粗大的碳化物网络,同时合金元素产生偏析,晶内生成了大块的合金渗碳体,使得它在室温下为脆性,机加工性能极差.喷射成形1.8C-1.6Al超高碳钢则利用喷射成形工艺冷却速度大的特点细化了晶粒,减小了珠光体的片间距,提高了硬度与强度,同时降低了元素偏析程度.同时发现:在铸态1.8C-1.6Al超高碳钢中和在喷射成形1.8C-1.6Al超高碳钢中Al在晶内的分布情况正好相反,由反偏析转变为正偏析.

  17. La0.6Pr0.4Fe11.4Si1.6B0.2合金及其氢化物的磁热效应%Magneto-Caloric Effect of La0.6Pr0.4Fe11.4Si1.6B0.2 Alloy and Its Hydride

    Institute of Scientific and Technical Information of China (English)

    葛玉梅; 松林; 黄焦宏; 刘翠兰; 张涛; 特古斯

    2013-01-01

    The preparation and magnetocaloric effects of the La0.6Pr0.4Fe11.4Si1.6B0.2 alloy and its hydride La0.6Pr0.4Fe11.4Si1.6B0.2Hy were investigated.The X-ray diffraction (XRD) and scanning electron microscopy (SEM) results showed that the microstructures of the La0.6Pr0.4Fe11.4Si1.6B0.2 alloy were composed of the NaZn13-type phase with space group of Fm-3c,La-rich phase and Fe-rich phase.The absorbing hydrogen content was determined by differential scanning calorimetry (DSC) method to be about y =1.7.The lattice constant increased from 1.2295 to 1.2491 nm,indicating lattice expansion.The magnetic measurement results showed that the Curie temperature of hydride La0.6Pr0.4Fe11.4Si1.6B0.2Hy increased from 198 to 325 K and this hydride aged at room temperature for 190 d maintained a large magnetocaloric effect,with a maximum magnetic entropy change of 9.1 J·kg-1·K-1 in a magnetic field of 1.5 T.%研究了La0.6Pr04Fe11.4Si1.6 B0.2合金及其氢化物La0.6 Pr0.4Fe11.4 Si1.6 B0.2Hy的制备工艺与磁热效应.室温XRD分析与SEM成分分析表明La0.6Pr0.4Fe11.4Si1.6B0.2合金主相为NaZn13型立方结构(空间群为Fm-3c),存在富La相(空间群为P4/nmm)与富Fe相.氢化物La0.6Pr0.4Fe11.4 Si1.6B0.2Hy的晶格常数a由合金的1.2295 nm增大到1.2491 nm.DSC测定氢化物的氢含量y约为1.7.磁性测量结果表明:氢化物La0.6Pro.4Fe11.4Si1.6B0.2Hy的居里温度Tc由合金的198 K增至325 K,提高了127 K.在0~1.5 T外磁场下合金与氢化物最大磁熵变-△SMmax均为9.1 J·kg-1·K-1.氢化物La0.6P0.4 F114 Si1.6B02Hy在室温下搁量190 d后物相与磁热效应基本保持不变.

  18. The dual effect of curcumin nanoparticles encapsulated by 1-3/1-6 β-glucan from medicinal mushrooms Hericium erinaceus and Ganoderma lucidum

    Science.gov (United States)

    Huong Le, Mai; Doan Do, Hai; Tran Thi, Hong Ha; Dung, Le Vu; Nguyen, Hoai Nam; Nhu Tran Thi, Hang; Dinh Nguyen, Luyen; Hoang, Chi Kim; Le, Huu Cuong; Huong Le Thi, Thu; Trinh, Hoang Trung; Thu Ha, Phuong

    2016-12-01

    Curcumin is a polyphenol from turmeric Curcuma longa L that has been proved to possess numerous biological and pharmaceutical activities, including anti-cancer properties. However, curcumin has only limited clinical applications due to the aqueous insolubility characteristic that reduces its biological availability. On the other hand, using nanoparticles as drug delivery system has potential as it increases solubility of hydrophobic substances such as curcumin. Furthermore, nanoparticles can protect and control release of drug. Therefore, the objective of this project is to prepare nanoparticles by polymeric encapsulating curcumin by 1-3/1-6 β-glucan extracted from Vietnamese mushrooms to increase drug delivery efficiency and biological effect. Method of the preparation is nano-precipitation. The produced curcumin-β-glucan-nanoparticles (NanoGluCur) takes spherical shape with 60-70 nm in diameter. As expected, water solubility of curcumin increases about 180 times, from 0.6 μg ml-1 to 0.11 mg ml-1. Loading capacity of NanoGluCur is 18.16%. In vitro cytotoxicity and anti-tumor promoting effects of NanoGluCur were also investigated. Results revealed that NanoGluCur is able to inhibit the growth of two human cancer cell lines Hep-G2 and LU-1 with IC50 values of 6.82 and 15.53 mg ml-1, respectively, while free curcumin expresses the activity with IC50 values of 7.41 and 18.82 mg ml-1. At the concentration of 40 mg ml-1, NanoGluCur showed anti-tumor promoting effects in reducing tumor size by 59.93% and tumor density by 40.52%, while the percentages caused by pristine curcumin were 41.36% and 29.14%, respectively. These results demonstrated dual effect of 1-3/1-6 β-glucan encapsulated curcumin nanoparticles: higher water solubility and better in vitro anti-cancer effects compared to free curcumin and 1-3/1-6 β-glucan, expectedly. This observation can potentially open a new approach in research and manufacture of functional foods from medicinal mushrooms.

  19. The independent prokaryotic origins of eukaryotic fructose-1, 6-bisphosphatase and sedoheptulose-1, 7-bisphosphatase and the implications of their origins for the evolution of eukaryotic Calvin cycle

    Directory of Open Access Journals (Sweden)

    Jiang Yong-Hai

    2012-10-01

    Full Text Available Abstract Background In the Calvin cycle of eubacteria, the dephosphorylations of both fructose-1, 6-bisphosphate (FBP and sedoheptulose-1, 7-bisphosphate (SBP are catalyzed by the same bifunctional enzyme: fructose-1, 6-bisphosphatase/sedoheptulose-1, 7-bisphosphatase (F/SBPase, while in that of eukaryotic chloroplasts by two distinct enzymes: chloroplastic fructose-1, 6-bisphosphatase (FBPase and sedoheptulose-1, 7-bisphosphatase (SBPase, respectively. It was proposed that these two eukaryotic enzymes arose from the divergence of a common ancestral eubacterial bifunctional F/SBPase of mitochondrial origin. However, no specific affinity between SBPase and eubacterial FBPase or F/SBPase can be observed in the previous phylogenetic analyses, and it is hard to explain why SBPase and/or F/SBPase are/is absent from most extant nonphotosynthetic eukaryotes according to this scenario. Results Domain analysis indicated that eubacterial F/SBPase of two different resources contain distinct domains: proteobacterial F/SBPases contain typical FBPase domain, while cyanobacterial F/SBPases possess FBPase_glpX domain. Therefore, like prokaryotic FBPase, eubacterial F/SBPase can also be divided into two evolutionarily distant classes (Class I and II. Phylogenetic analysis based on a much larger taxonomic sampling than previous work revealed that all eukaryotic SBPase cluster together and form a close sister group to the clade of epsilon-proteobacterial Class I FBPase which are gluconeogenesis-specific enzymes, while all eukaryotic chloroplast FBPase group together with eukaryotic cytosolic FBPase and form another distinct clade which then groups with the Class I FBPase of diverse eubacteria. Motif analysis of these enzymes also supports these phylogenetic correlations. Conclusions There are two evolutionarily distant classes of eubacterial bifunctional F/SBPase. Eukaryotic FBPase and SBPase do not diverge from either of them but have two independent origins

  20. The independent prokaryotic origins of eukaryotic fructose-1, 6-bisphosphatase and sedoheptulose-1, 7-bisphosphatase and the implications of their origins for the evolution of eukaryotic Calvin cycle.

    Science.gov (United States)

    Jiang, Yong-Hai; Wang, De-Yong; Wen, Jian-Fan

    2012-10-22

    In the Calvin cycle of eubacteria, the dephosphorylations of both fructose-1, 6-bisphosphate (FBP) and sedoheptulose-1, 7-bisphosphate (SBP) are catalyzed by the same bifunctional enzyme: fructose-1, 6-bisphosphatase/sedoheptulose-1, 7-bisphosphatase (F/SBPase), while in that of eukaryotic chloroplasts by two distinct enzymes: chloroplastic fructose-1, 6-bisphosphatase (FBPase) and sedoheptulose-1, 7-bisphosphatase (SBPase), respectively. It was proposed that these two eukaryotic enzymes arose from the divergence of a common ancestral eubacterial bifunctional F/SBPase of mitochondrial origin. However, no specific affinity between SBPase and eubacterial FBPase or F/SBPase can be observed in the previous phylogenetic analyses, and it is hard to explain why SBPase and/or F/SBPase are/is absent from most extant nonphotosynthetic eukaryotes according to this scenario. Domain analysis indicated that eubacterial F/SBPase of two different resources contain distinct domains: proteobacterial F/SBPases contain typical FBPase domain, while cyanobacterial F/SBPases possess FBPase_glpX domain. Therefore, like prokaryotic FBPase, eubacterial F/SBPase can also be divided into two evolutionarily distant classes (Class I and II). Phylogenetic analysis based on a much larger taxonomic sampling than previous work revealed that all eukaryotic SBPase cluster together and form a close sister group to the clade of epsilon-proteobacterial Class I FBPase which are gluconeogenesis-specific enzymes, while all eukaryotic chloroplast FBPase group together with eukaryotic cytosolic FBPase and form another distinct clade which then groups with the Class I FBPase of diverse eubacteria. Motif analysis of these enzymes also supports these phylogenetic correlations. There are two evolutionarily distant classes of eubacterial bifunctional F/SBPase. Eukaryotic FBPase and SBPase do not diverge from either of them but have two independent origins: SBPase share a common ancestor with the gluconeogenesis

  1. 1,6-二磷酸果糖治疗重度心力衰竭%1,6-disphosphofructose in Treatment of Severe Heart Failure

    Institute of Scientific and Technical Information of China (English)

    卢延生; 卢春生; 李中言

    2001-01-01

    目的探讨 1,6-二磷酸果糖(FDP)治疗重度心力衰竭(CHF)的临床疗效。方法 62 例重度 CHF 患者,随机分为治疗组(32 例)和对照组(30 例),对照组常规应用洋地黄、利尿剂、转换酶抑制剂等进行治疗;治疗组在此基础上加用 FDP 治疗。结果治疗组总有效率 87.5 %(28 例),对照组总有效率 70.0 %(21 例),组间比较有显著性差异(P<0.05);心脏超声检查治疗组与对照组比较各项心功能检查指标亦有显著性差异(P<0.05)。结论 FDP 与洋地黄、利尿剂、转换酶抑制剂联合治疗重度 CHF 疗效显著。%Objective To research the clinical effect of 1,6-disphosphofrutose on severe heart failure.Methods Sixty two cases with severe heart failure were randomly divided into the treatment group(32 cases) and the control group(30 cases).In the control group digitalis,diurtic and transfering enzyme inhibiters were used for routine treatment while in the treatment group 1,6-disphosphofructose was also used besides those above.Results The total effect rates were respectively 87.5%(28 cases) in the treatment group and 70.0%(21 cases)in the control group, and there were great differences between 2 groups(P<0.05); according to ultrasonic examination,there were also great differences in every heart index between 2 groups(P<0.05).Conclusion 1,6-disphosphofructose combined with digitalis,diuretic and transfering inhibiters has a good effect on severe heart failure.

  2. The cell-permeable Aβ1-6A2VTAT(D) peptide reverts synaptopathy induced by Aβ1-42wt.

    Science.gov (United States)

    Cimini, Sara; Sclip, Alessandra; Mancini, Simona; Colombo, Laura; Messa, Massimo; Cagnotto, Alfredo; Di Fede, Giuseppe; Tagliavini, Fabrizio; Salmona, Mario; Borsello, Tiziana

    2016-05-01

    Alzheimer disease (AD) is the most prevalent form of dementia. Loss of hippocampal synapses is the first neurodegenerative event in AD. Synaptic loss has been associated with the accumulation in the brain parenchyma of soluble oligomeric forms of amyloid β peptide (Aβ1-42wt). Clinical observations have shown that a mutation in the APP protein (A673V) causes an early onset AD-type dementia in homozygous carriers while heterozygous carriers are unaffected. This mutation leads to the formation of mutated Aβ peptides (Aβ1-42A2V) in homozygous patients, while in heterozygous subjects both Aβ1-42wt and Aβ1-42A2V are present. To better understand the impact of the A673V mutation in AD, we analyzed the synaptotoxic effect of oligomers formed by aggregation of different Aβ peptides (Aβ1-42wt or Aβ1-42A2V) and the combination of the two Aβ1-42MIX (Aβ1-42wt and Aβ1-42A2V) in an in vitro model of synaptic injury. We showed that Aβ1-42A2V oligomers are more toxic than Aβ1-42wt oligomers in hippocampal neurons, confirming the results previously obtained in cell lines. Furthermore, we reported that oligomers obtained by the combination of both wild type and mutated peptides (Aβ1-42MIX) did not exert synaptic toxicity. We concluded that the combination of Aβ1-42wt and Aβ1-42A2V peptides hinders the toxicity of Aβ1-42A2V and counteracts the manifestation of synaptopathy in vitro. Finally we took advantage of this finding to generate a cell-permeable peptide for clinical application, by fusing the first six residues of the Aβ1-42A2V to the TAT cargo sequence (Aβ1-6A2VTAT(D)). Noteworthy, the treatment with Aβ1-6A2VTAT(D) confers neuroprotection against both in vitro and in vivo synaptopathy models. Therefore Aβ1-6A2VTAT(D) may represent an innovative therapeutic tool to prevent synaptic degeneration in AD.

  3. Cosmological Measures without Volume Weighting

    CERN Document Server

    Page, Don N

    2008-01-01

    Many cosmologists (myself included) have advocated volume weighting for the cosmological measure problem, weighting spatial hypersurfaces by their volume. However, this often leads to the Boltzmann brain problem, that almost all observations would be by momentary Boltzmann brains that arise very briefly as quantum fluctuations in the late universe when it has expanded to a huge size, so that our observations (too ordered for Boltzmann brains) would be highly atypical and unlikely. Here it is suggested that volume weighting may be a mistake. Volume averaging is advocated as an alternative. One consequence would be a loss of the argument for eternal inflation.

  4. Heliophysics 3 Volume Set

    Science.gov (United States)

    Schrijver, Carolus J.; Siscoe, George L.

    2010-11-01

    Volume 1: Preface; 1. Prologue Carolus J. Schrijver and George L. Siscoe; 2. Introduction to heliophysics Thomas J. Bogdan; 3. Creation and destruction of magnetic field Matthias Rempel; 4. Magnetic field topology Dana W. Longcope; 5. Magnetic reconnection Terry G. Forbes; 6. Structures of the magnetic field Mark B. Moldwin, George L. Siscoe and Carolus J. Schrijver; 7. Turbulence in space plasmas Charles W. Smith; 8. The solar atmosphere Viggo H. Hansteen; 9. Stellar winds and magnetic fields Viggo H. Hansteen; 10. Fundamentals of planetary magnetospheres Vytenis M. Vasyliūnas; 11. Solar-wind magnetosphere coupling: an MHD perspective Frank R. Toffoletto and George L. Siscoe; 12. On the ionosphere and chromosphere Tim Fuller-Rowell and Carolus J. Schrijver; 13. Comparative planetary environments Frances Bagenal; Bibliography; Index. Volume 2: Preface; 1. Perspective on heliophysics George L. Siscoe and Carolus J. Schrijver; 2. Introduction to space storms and radiation Sten Odenwald; 3. In-situ detection of energetic particles George Gloeckler; 4. Radiative signatures of energetic particles Tim Bastian; 5. Observations of solar and stellar eruptions, flares, and jets Hugh Hudson; 6. Models of coronal mass ejections and flares Terry Forbes; 7. Shocks in heliophysics Merav Opher; 8. Particle acceleration in shocks Dietmar Krauss-Varban; 9. Energetic particle transport Joe Giacalone; 10. Energy conversion in planetary magnetospheres Vytenis Vasyliūnas; 11. Energization of trapped particles Janet Green; 12. Flares, CMEs, and atmospheric responses Tim Fuller-Rowell and Stanley C. Solomon; 13. Energetic particles and manned spaceflight 358 Stephen Guetersloh and Neal Zapp; 14. Energetic particles and technology Alan Tribble; Appendix I. Authors and editors; List of illustrations; List of tables; Bibliography; Index. Volume 3: Preface; 1. Interconnectedness in heliophysics Carolus J. Schrijver and George L. Siscoe; 2. Long-term evolution of magnetic activity of Sun

  5. Improvement of critical current density in thallium-based (Tl,Bi)Sr(1.6)Ba(0.4)Ca2Cu3O(x) superconductors

    Science.gov (United States)

    Ren, Z. F.; Wang, C. A.; Wang, J. H.; Miller, D. J.; Goretta, K. C.

    1995-01-01

    Epitaxial (Tl,Bi)Sr(1.6)Ba(0.4)Ca2Cu3O(x) ((Tl,Bi)-1223) thin films on (100) single crystal LaAlO3 substrates were synthesized by a two-step procedure. Phase development, microstructure, and relationships between film and substrate were studied by X-ray diffraction (XRD), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). Resistance versus temperature, zero-field-cooled and field cooled magnetization, and transport critical current density (J(sub c)) were measured. The zero-resistance temperature was 105-111 K. J(sub c) at 77 K and zero field was greater than 2 x 10(exp 6) A/sq cm. The films exhibited good flux pinning properties.

  6. MicroRNA-122 antagonism against hepatitis C virus genotypes 1-6 and reduced efficacy by host RNA insertion or mutations in the HCV 5' UTR

    DEFF Research Database (Denmark)

    Li, Yiping; Gottwein, Judith; Scheel, Troels Kasper Høyer

    2011-01-01

    , 1b, 2a, 2b, 3a, 4a, 5a, and 6a with efficient growth in Huh7.5 cells. Deletion mutagenesis studies demonstrated that the 5' UTR SLI was essential for genotypes 1-6 infection. However, lack of SLI could be compensated for by insertion of other structured HCV or host RNA sequences, including U3 small......; this was attributed to the lack of an intact S1 by reverse genetics studies. Therefore, we engineered the corresponding U3 RNA sequences into S1 and demonstrated that HCV recombinants with wild-type SLI and single or combined mutations at four of eight nucleotides of S1 were viable in Huh7.5 cells. These mutations...

  7. K-components for the approx. 1. 4-, approx. 1. 6-, and approx. 1. 7-MeV structures in the fission of /sup 232/Th + n

    Energy Technology Data Exchange (ETDEWEB)

    Auchampaugh, G.F.; Plattard, S.; Hill, N.W.; de Saussure, G.; Perez, R.B.; Harvey, J.A.

    1981-07-01

    Neutron-induced angle-integrated fission cross sections of /sup 232/Th were measured from 0.7 to 9 MeV with a nominal neutron energy resolution of 0.15 ns/m. Data were taken for the angular intervals 0 to 23.4/sup 0/, 0 to 33.7/sup 0/, 0 to 51.7/sup 0/, and 0 to 90/sup 0/. The structures at approx. 1.4, approx. 1.6, and approx. 1.7 MeV were interpreted in terms of rotational bands with K = 1/2, 3/2, and greater than or equal to 5/2. The approximate relative fission strengths for the K-bonds are in the proportion 1.7:2.4:1.0, 0.0:2.6:1.0, and 1.0:2.8:0.0 for the three structures, respectively.

  8. Facile Synthesis of 1,6-Bis(2-furyl-2,5-bis(2-hydroxy-3-formyl-5-methylbenzyl-2,5-diazahexane: a New Dinucleating Ligand

    Directory of Open Access Journals (Sweden)

    Chang-Rang Liu

    2001-11-01

    Full Text Available A convenient three-step preparation of the dinucleating ligand, 1,6-bis(2-furyl-2,5-bis(2-hydroxy-3-formyl-5-methylbenzyl-2,5-diazahexane (3 starting from 2,6-bis(hydroxymethyl-4-methylphenol (4 is reported. Compound 4 was partially oxidized with preactivated manganese dioxide to form compound 5, which was converted to 2-hydroxy-3-chloromethyl-5-ethylbenzaldehyde (6 with conc.HCl/EtOH. Compound 6 in turn reacted with N,N’-bis (2-furyl-1,2-diaminoethane (7 in the presence of K2CO3 in ethanol to give the title compound 3. No protecting groups were required in the whole process and the conditions were mild.

  9. Effect of gaseous and solid simulated jet plumes on a 040A space shuttle launch configuration at Mach numbers from 1.6 to 2.2

    Science.gov (United States)

    Lanfranco, M. J.; Sparks, V. W.; Kavanaugh, A. T.

    1973-01-01

    An experimental investigation was conducted in a 9- by 7-foot supersonic wind tunnel to determine the effect of plume-induced flow separation and aspiration effects due to operation of both the orbiter and the solid rocket motors on a 0.019-scale model of the launch configuration of the space shuttle vehicle. Longitudinal and lateral-directional stability data were obtained at Mach numbers of 1.6, 2.0, and 2.2 with and without the engines operating. The plumes exiting from the engines were simulated by a cold gas jet supplied by an auxiliary 200 atmosphere air supply system, and by solid body plume simulators. Comparisons of the aerodynamic effects produced by these two simulation procedures are presented. The data indicate that the parameters most significantly affected by the jet plumes are the pitching moment, the elevon control effectiveness, the axial force, and the orbiter wing loads.

  10. Alkyl levulinates as `green chemistry' precursors: butane-1,4-diyl bis(4-oxopentanoate) and hexane-1,6-diyl bis(4-oxopentanoate).

    Science.gov (United States)

    Gainsford, Graeme J; Hinkley, Simon

    2013-06-01

    Levulinic acid derivatives are potential `green chemistry' renewably sourced molecules with utility in industrial coatings applications. Suitable single crystals of the centrosymmetric title compounds, C14H22O6 and C16H26O6, respectively, were obtained with difficulty. The data for the latter hexane-1,6-diyl compound were extracted from the major fragment of a three-component twinned crystal. Both compounds crystallize in similar-sized unit cells with identical symmetry, utilizing the same weak nonconventional attractive C-H···O(ketone) hydrogen bonds via C(4) and C(5) motifs, which expand to R(2)(2)(30) ring and C(2)(2)(14) chain motifs. Their different packing orientations in similar-sized unit cells suggest that crystal growth involving packing mixes could lead to intergrowths or twins.

  11. Síntesis y evaluación del efecto ansiolítico de un nuevo derivado pirimido[1,6-a]pirimidínico

    OpenAIRE

    Alejandro Muñoz Zurita; Jesús Sandoval Ramírez; Luis Emilio Espinoza Moreno; Carmen Parra Cid; Luis Alberto Juárez Leyva; Daniel Limón Pérez de León; Gonzalo Flores

    2003-01-01

    Se describe la síntesis de la (E)-9-estirilcarboxamido-6,8- dioxo-7-propil-1,3,4,6,7,8-hexahidro-2H-pirimido[1,6-a]pirimidina (9) a partir de 6-aminouracilo (4) utilizando una vía de síntesis quimio- y regioselectiva; además se reporta el estudio fármaco-biológico de 9 utilizando el modelo experimental ansiolítico del laberinto elevado en cruz. Adicionalmente, se presenta la relación dosis efecto de 9 sobre la actividad locomotora empleando el modelo de campo cerrado en ratas macho Wistar; la...

  12. Analyzing powers for the inclusive reaction of deuterons on carbon at energies between 0.175 and 1.6 GeV

    Energy Technology Data Exchange (ETDEWEB)

    Ladygin, V.P. [Laboratoire National Saturne, Centre d`Etudes de Saclay, 91 -Gif-sur-Yvette (France)]|[JINR-LHE, 141980, Dubna, Moscow region (Russian Federation); Tomasi-Gustafsson, E.; Ball, J. [Laboratoire National Saturne, Centre d`Etudes de Saclay, 91 - Gif-sur-Yvette (France)]|[CEA/DSM DAPNIA-SPhN, CE Saclay, F-91191-Gif-sur-Yvette (France); Bimbot, L.; Bisson, Y.; Boyard, J.L.; Courtat, P.; Gacougnolle, R.; Hennino, T.; Jones, M.K.; Skowron, R. [CNRS/IN2P3 IPN, 91400 Orsay (France); Boivin, M.; Kunne, R.A. [Laboratoire National Saturne, Centre d`Etudes de Saclay, 91 - Gif-sur-Yvette (France); Cheung, N.E.; Perdrisat, C.F. [The College of William and Mary, Williamsburg, VA 23187 (United States); Piskunov, N.M.; Sitnik, I.M.; Strokovsky, E.A. [JINR-LHE, 141980, Dubna, Moscow region (Russian Federation); Punjabi, V. [Norfolk State University, Norfolk, VA 23504 (United States); Zupranski, P. [Soltan Institute for Nuclear Studies, 00-681 Warsaw (Poland)

    1998-02-11

    The results of a new calibration of the polarimeter POMME for energies between 0.175 and 1.6 GeV, using the polarized deuteron beam of the laboratoire national Saturne, are reported. The present calibration, together with previous ones, gives a complete set of vector analyzing powers in the energy range 0.175-1.8 GeV. The vector analyzing powers are fitted as a function of energy and scattering angle, with empirical formulae. Two sets of parameters are given, one for deuteron energies between 0.175 and 0.575 GeV, and the other in an energy range between 0.7 and 1.8 GeV. (orig.). 13 refs.

  13. Studies on the photochemistry of 1,7-diphenyl-1,6-heptadiene-3,5-dione, a non-phenolic curcuminoid model.

    Science.gov (United States)

    Sundaryono, Agus; Nourmamode, Aziz; Gardrat, Christian; Grelier, Stéphane; Bravic, Georges; Chasseau, Daniel; Castellan, Alain

    2003-09-01

    The comparative photostability of curcumin 1, and two non-phenolic curcuminoids: 1,7-diphenyl-1,6-heptadiene-3,5-dione 2 (unsubstituted curcumin) and dimethylcurcumin 3 in non-degassed dilute solutions (approximately 3-5 x 10(-5) mol l(-1)) has been established by UV-visible absorption spectroscopy; disappearance quantum yields were measured. The similar behavior of the three studied curcuminoids is indicative of only a moderate role of phenol groups in the photodegradation process. Structural analysis of the photodegradation products of compound 2 in more concentrated solution (approximately 3.6 x 10(-3) mol l(-1)) shows formation of benzaldehyde, cinnamaldehyde, 2'-hydroxy-5',6'-benzochalcone 4, flavanone 5 and some other unidentified photoproducts. Flavanone 5 is formed by irradiation of chalcone 4. It represents a unique example of photochemical conversion of a diarylheptanoid molecule into a flavonoid, another very important class of natural products.

  14. Effect of ScAl/sub 3/ phase dispersity on hardening of A1-6. 3% Mg-0. 21% Sc alloy

    Energy Technology Data Exchange (ETDEWEB)

    Drits, M.E.; Bykov, Y.G.; Toropova, L.S.

    1985-09-01

    This paper reports on attempts to evaluate the quantitatively separate influence exerted on the hardening of an aluminum alloy containing 6.3% Mg and 0.21% Sc by both particles of the ScAl/sub 3/ phase exhibiting a different degree of dispersity and also the substructure which is formed in the alloy after hot deformation. The binary aluminum alloy containing 6.3% of Mg after hot deformation has a fully recrystallized structure, while the aluminum alloy containing 6.5% of Mg and Sc is recrystallized. It is concluded that: the magnitude of substructure hardening of the A1-6.3% Mg-0.21% Sc alloy is similar, irrespective of the dispersity of coherent ScAl/sub 3/ particles; and, hardening of the alloy, which is governed by coherent ScAl/sub 3/-phase segregations less than 20 mm in diameter, significantly exceeds the effect of substructure hardening.

  15. 1, 6-diisocyanatohexane-extended poly (1, 4-butylene succinate / hydroxyl apatite nano particle scaffolds: Potential materials for bone regeneration applications

    Science.gov (United States)

    Kaur, Kulwinder; Singh, K. J.; Anand, Vikas; Bhatia, Gaurav; Nim, Lovedeep; Kaur, Manpreet; Arora, Daljit Singh

    2017-05-01

    Bioresorbable and bioactive scaffolds are promising materials for various biomedical applications including bone regeneration and drug delievrery. Authors present bioactive scaffolds prepared from 1, 6-diisocyanatohexane-extended poly (1, 4-butylene succinate) (PBSu-DCH) with different amount of hydroxyl apatite nanoparticles (nHAp) by solvent casting and particulate leaching techniques. Different weight ratios of nHAp (i.e. 0, 5 and 10 wt %) with fixed weight ratio (i.e. 10 wt %) of PBSu-DCH polymer have been prepared. Scaffolds have been assessed for their morphology, bioactivity, degradation, drug release and biological properties including cytotoxicity, cell attachment using MG-63 cell line and antimicrobial activity. Effectual drug release has been measured by incorporating gentamycin as an antibiotic in the scaffolds. The study is aimed at developing new biodegradable scaffolds to be used in skull, jaw and tooth socket for preserving bone mass.

  16. A HIGHER EFFICIENCY OF CONVERTING GAS TO STARS PUSHES GALAXIES AT z ∼ 1.6 WELL ABOVE THE STAR-FORMING MAIN SEQUENCE

    Energy Technology Data Exchange (ETDEWEB)

    Silverman, J. D.; Rujopakarn, W. [Kavli Institute for the Physics and Mathematics of the Universe (WPI), The University of Tokyo Institutes for Advanced Study, The University of Tokyo, Kashiwa, Chiba 277-8583 (Japan); Daddi, E.; Liu, D. [Laboratoire AIM, CEA/DSM-CNRS-Universite Paris Diderot, Irfu/Service d’Astrophysique, CEA Saclay (France); Rodighiero, G. [Dipartimento di Fisica e Astronomia, Universita di Padova, vicolo Osservatorio, 3, I-35122 Padova (Italy); Sargent, M. [Astronomy Centre, Department of Physics and Astronomy, University of Sussex, Brighton BN1 9QH (United Kingdom); Renzini, A. [Instituto Nazionale de Astrofisica, Osservatorio Astronomico di Padova, v.co dell’Osservatorio 5, I-35122 Padova (Italy); Feruglio, C. [IRAM—Institut de RadioAstronomie Millimétrique, 300 rue de la Piscine, F-38406 Saint Martin d’Hères (France); Kashino, D. [Division of Particle and Astrophysical Science, Graduate School of Science, Nagoya University, Nagoya 464-8602 (Japan); Sanders, D. [Institute for Astronomy, University of Hawaii, 2680 Woodlawn Drive, Honolulu, HI 96822 (United States); Kartaltepe, J. [National Optical Astronomy Observatory, 950 N. Cherry Avenue, Tucson, AZ 85719 (United States); Nagao, T. [Graduate School of Science and Engineering, Ehime University, 2-5 Bunkyo-cho, Matsuyama 790-8577 (Japan); Arimoto, N. [Subaru Telescope, 650 North A’ohoku Place, Hilo, HI-96720 (United States); Berta, S.; Lutz, D. [Max-Planck-Institut für extraterrestrische Physik, D-84571 Garching (Germany); Béthermin, M. [European Southern Observatory, Karl-Schwarzschild-Strasse 2, D-85748 Garching (Germany); Koekemoer, A., E-mail: john.silverman@ipmu.jp [Space Telescope Science Institute, 3700 San Martin Drive, Baltimore, MD, 21218 (United States); and others

    2015-10-20

    Local starbursts have a higher efficiency of converting gas into stars, as compared to typical star-forming galaxies at a given stellar mass, possibly indicative of different modes of star formation. With the peak epoch of galaxy formation occurring at z > 1, it remains to be established whether such an efficient mode of star formation is occurring at high redshift. To address this issue, we measure the molecular gas content of seven high-redshift (z ∼ 1.6) starburst galaxies with the Atacama Large Millimeter/submillimeter Array and IRAM/Plateau de Bure Interferometer. Our targets are selected from the sample of Herschel far-infrared-detected galaxies having star formation rates (∼300–800 M{sub ⊙} yr{sup −1}) elevated (≳4×) above the star-forming main sequence (MS) and included in the FMOS-COSMOS near-infrared spectroscopic survey of star-forming galaxies at z ∼ 1.6 with Subaru. We detect CO emission in all cases at high levels of significance, indicative of high gas fractions (∼30%–50%). Even more compelling, we firmly establish with a clean and systematic selection that starbursts, identified as MS outliers, at high redshift generally have a lower ratio of CO to total infrared luminosity as compared to typical MS star-forming galaxies, although with a smaller offset than expected based on past studies of local starbursts. We put forward a hypothesis that there exists a continuous increase in star formation efficiency with elevation from the MS with galaxy mergers as a possible physical driver. Along with a heightened star formation efficiency, our high-redshift sample is similar in other respects to local starbursts, such as being metal rich and having a higher ionization state of the interstellar medium.

  17. 康莱特联合5-Fu对肝癌细胞Heap1-6的作用

    Institute of Scientific and Technical Information of China (English)

    刘燕燕; 赵和平

    2010-01-01

    目的研究康莱特(KLT)联用半量最佳体外抑瘤浓度5-氟尿嘧啶(5-Fu)、单药全量5-Fu对体外小鼠Heap1-6肝癌细胞增殖及凋亡的影响。方法采用MTT法检测各药物组对细胞活性的影响;采用流式细胞术分析各药物组对肝癌细胞株Hepa1-6细胞周期的影响。结果 MTT示:康莱特联用半量5-Fu较单药全量5-Fu的抗肿瘤效果显著;高剂量康莱特联合半量5-Fu的抗肝癌效果较低、中剂量组联合组显著。流式细胞术示:不同浓度的康莱特联合半量5-Fu较全量5-Fu的促凋亡作用差异在中剂量时差异无显著性。结论中剂量的康莱特联用半量5-Fu对肿瘤细胞的抑制作用及促凋亡作用与单药5-Fu的疗效相当,可以指导临床用药。

  18. Loss of α1,6-Fucosyltransferase Decreases Hippocampal Long Term Potentiation: IMPLICATIONS FOR CORE FUCOSYLATION IN THE REGULATION OF AMPA RECEPTOR HETEROMERIZATION AND CELLULAR SIGNALING.

    Science.gov (United States)

    Gu, Wei; Fukuda, Tomohiko; Isaji, Tomoya; Hang, Qinglei; Lee, Ho-hsun; Sakai, Seiichiro; Morise, Jyoji; Mitoma, Junya; Higashi, Hideyoshi; Taniguchi, Naoyuki; Yawo, Hiromu; Oka, Shogo; Gu, Jianguo

    2015-07-10

    Core fucosylation is catalyzed by α1,6-fucosyltransferase (FUT8), which transfers a fucose residue to the innermost GlcNAc residue via α1,6-linkage on N-glycans in mammals. We previously reported that Fut8-knock-out (Fut8(-/-)) mice showed a schizophrenia-like phenotype and a decrease in working memory. To understand the underlying molecular mechanism, we analyzed early form long term potentiation (E-LTP), which is closely related to learning and memory in the hippocampus. The scale of E-LTP induced by high frequency stimulation was significantly decreased in Fut8(-/-) mice. Tetraethylammonium-induced LTP showed no significant differences, suggesting that the decline in E-LTP was caused by postsynaptic events. Unexpectedly, the phosphorylation levels of calcium/calmodulin-dependent protein kinase II (CaMKII), an important mediator of learning and memory in postsynapses, were greatly increased in Fut8(-/-) mice. The expression levels of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate receptors (AMPARs) in the postsynaptic density were enhanced in Fut8(-/-) mice, although there were no significant differences in the total expression levels, implicating that AMPARs without core fucosylation might exist in an active state. The activation of AMPARs was further confirmed by Fura-2 calcium imaging using primary cultured neurons. Taken together, loss of core fucosylation on AMPARs enhanced their heteromerization, which increase sensitivity for postsynaptic depolarization and persistently activate N-methyl-d-aspartate receptors as well as Ca(2+) influx and CaMKII and then impair LTP. © 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

  19. Identification and expression of fructose-1,6-bisphosphate aldolase genes and their relations to oil content in developing seeds of tea oil tree (Camellia oleifera).

    Science.gov (United States)

    Zeng, Yanling; Tan, Xiaofeng; Zhang, Lin; Jiang, Nan; Cao, Heping

    2014-01-01

    Tea oil tree (Camellia oleifera, Co) provides a fine edible oil source in China. Tea oil from the seeds is very beneficial to human health. Fructose-1,6-bisphosphate aldolase (FBA) hydrolyzes fructose-1,6-bisphosphate into dihydroxyacetone phosphate and glyceraldehyde 3-phosphate, two critical metabolites for oil biosynthesis. The objectives of this study were to identify FBA genes and investigate the relationship between FBA gene expression and oil content in developing seeds of tea oil tree. In this paper, four developmentally up-regulated CoFBA genes were identified in Camellia oleifera seeds based on the transcriptome from two seed developmental stages corresponding to the initiation and peak stages of lipid biosynthesis. The expression of CoFBA genes, along with three key oil biosynthesis genes CoACP, CoFAD2 and CoSAD were analyzed in seeds from eight developmental stages by real-time quantitative PCR. The oil content and fatty acid composition were also analyzed. The results showed that CoFBA and CoSAD mRNA levels were well-correlated with oil content whereas CoFAD2 gene expression levels were correlated with fatty acid composition in Camellia seeds. We propose that CoFBA and CoSAD are two important factors for determining tea oil yield because CoFBA gene controls the flux of key intermediates for oil biosynthesis and CoSAD gene controls the synthesis of oleic acid, which accounts for 80% of fatty acids in tea oil. These findings suggest that tea oil yield could be improved by enhanced expression of CoFBA and CoSAD genes in transgenic plants.

  20. Identification and expression of fructose-1,6-bisphosphate aldolase genes and their relations to oil content in developing seeds of tea oil tree (Camellia oleifera.

    Directory of Open Access Journals (Sweden)

    Yanling Zeng

    Full Text Available Tea oil tree (Camellia oleifera, Co provides a fine edible oil source in China. Tea oil from the seeds is very beneficial to human health. Fructose-1,6-bisphosphate aldolase (FBA hydrolyzes fructose-1,6-bisphosphate into dihydroxyacetone phosphate and glyceraldehyde 3-phosphate, two critical metabolites for oil biosynthesis. The objectives of this study were to identify FBA genes and investigate the relationship between FBA gene expression and oil content in developing seeds of tea oil tree. In this paper, four developmentally up-regulated CoFBA genes were identified in Camellia oleifera seeds based on the transcriptome from two seed developmental stages corresponding to the initiation and peak stages of lipid biosynthesis. The expression of CoFBA genes, along with three key oil biosynthesis genes CoACP, CoFAD2 and CoSAD were analyzed in seeds from eight developmental stages by real-time quantitative PCR. The oil content and fatty acid composition were also analyzed. The results showed that CoFBA and CoSAD mRNA levels were well-correlated with oil content whereas CoFAD2 gene expression levels were correlated with fatty acid composition in Camellia seeds. We propose that CoFBA and CoSAD are two important factors for determining tea oil yield because CoFBA gene controls the flux of key intermediates for oil biosynthesis and CoSAD gene controls the synthesis of oleic acid, which accounts for 80% of fatty acids in tea oil. These findings suggest that tea oil yield could be improved by enhanced expression of CoFBA and CoSAD genes in transgenic plants.

  1. Creep and microstructural processes in a low-alloy 2.25%Cr1.6%W steel (ASTM Grade 23)

    Energy Technology Data Exchange (ETDEWEB)

    Kucharova, K. [Institute of Physics of Materials, Academy of Sciences of the Czech Republic, CZ-616 62 Brno (Czech Republic); Sklenicka, V., E-mail: sklen@ipm.cz [Institute of Physics of Materials, Academy of Sciences of the Czech Republic, CZ-616 62 Brno (Czech Republic); CEITEC — IPM, Institute of Physics of Materials, Academy of Sciences of the Czech Republic, CZ-616 62 Brno (Czech Republic); Kvapilova, M.; Svoboda, M. [Institute of Physics of Materials, Academy of Sciences of the Czech Republic, CZ-616 62 Brno (Czech Republic); CEITEC — IPM, Institute of Physics of Materials, Academy of Sciences of the Czech Republic, CZ-616 62 Brno (Czech Republic)

    2015-11-15

    A low-alloy 2.25%Cr1%Mo steel (ASTM Grade 22) has been greatly improved by the substitution of almost all of the 1%Mo by 1.6%W. The improved material has been standardized as P/T23 steel (Fe–2.25Cr–1.6W–0.25V–0.05Nb–0.07C). The present investigation was conducted on T23 steel in an effort to obtain a more complete description and understanding of the role of the microstructural evolution and deformation processes in high-temperature creep. Constant load tensile creep tests were carried out in an argon atmosphere in the temperature range 500–650 °C at stresses ranging from 50 to 400 MPa. It was found that the diffusion in the matrix lattice is the creep-rate controlling process. The results of an extensive transmission electron microscopy (TEM) analysis programme to investigate microstructure evolution as a function of temperature are described and compared with the thermodynamic calculations using the software package Thermo-Calc. The significant creep-strength drop of T23 steel after long-term creep exposures can be explained by the decrease in dislocation hardening, precipitation hardening and solid solution hardening due to the instability of the microstructure at high temperature. - Highlights: • The constant load creep tests of T23 steel were carried out at 500–650 °C. • The stress exponents of the creep rate correspond to power law (dislocation) creep. • Diffusion in the matrix lattice is the creep-rate controlling process. • The microstructure instability is the main creep degradation process in T23 steel.

  2. Study on Precursor Activity of the X1.6 Flare in the Great AR 12192 with SDO, IRIS, and Hinode

    Science.gov (United States)

    Bamba, Yumi; Lee, Kyoung-Sun; Imada, Shinsuke; Kusano, Kanya

    2017-05-01

    The physical properties and their contribution to the onset of a solar flare are still uncleare even though chromospheric brightening is considered a precursor phenomenon of a flare. Many studies suggested that photospheric magnetic field changes cause destabilization of large-scale coronal structure. We aim to understand how a small photospheric change contributes to a flare and to reveal how the intermediary chromosphere behaves in the precursor phase. We analyzed the precursor brightening of the X1.6 flare on 2014 October 22 in the AR 12192 using the Interface Region Imaging Spectrograph (IRIS) and Hinode/EUV Imaging Spectrometer (EIS) data. We investigated a localized jet with the strong precursor brightening, and compared the intensity, Doppler velocity, and line width in C ii, Mg ii k, and Si iv lines by IRIS and He ii, Fe xii, and Fe xv lines by Hinode/EIS. We also analyzed the photospheric magnetic field and chromospheric/coronal structures using the Solar Dynamics Observatory (SDO)/Helioseismic and Magnetic Imager and Atmospheric Imaging Assembly. We found a significant blueshift (˜100 km s-1), which is related to the strong precursor brightening over a characteristic magnetic field structure, and the blueshift was observed at all of the temperatures. This might indicate that the flow is accelerated by Lorentz force. Moreover, the large-scale coronal loop that connects the foot points of the flare ribbons was destabilized just after the precursor brightening with the blueshift. It suggests that magnetic reconnection locally occurred in the lower chromosphere and it triggered magnetic reconnection of the X1.6 flare in the corona.

  3. Effect of Ag additions on the Bi1.6Pb0.4Sr2Co1.8Ox thermoelectric properties

    Directory of Open Access Journals (Sweden)

    Diez, J. C.

    2013-04-01

    Full Text Available Bi1.6Pb0.4Sr2Co1.8Ox thermoelectric ceramics with small Ag additions (0, 1, and 3 wt.% have been successfully produced by a sol-gel method via nitrates. Microstructure has shown a reduction on the amount of secondary phases and an increase on the bulk density with increasing Ag contents. The microstructural evolution, as a function of Ag content, is confirmed with the electrical resistivity values which show an important decrease for the 3 wt.% Ag samples, leading to maximum power factor values of about 0.025 mW/K2.m at room temperature, which is about two times higher than the obtained for the Ag-free sintered samples.Se han preparado cerámicas termoeléctricas de composición Bi1.6Pb0.4Sr2Co1.8Ox con pequeñas adiciones de Ag (0, 1, y 3 % en peso por medio de un método sol-gel a partir de los nitratos metálicos. La microestructura ha mostrado una reducción de la cantidad de fases secundarias y un aumento de la densidad del material al aumentar la cantidad de Ag. La evolución microestructural, en función del contenido de Ag, se ha confirmado con los valores de la resistividad eléctrica que muestra una reducción importante para las muestras con 3 % en peso de Ag, lo que lleva a alcanzar valores máximos del factor de potencia de alrededor de 0.025 mW/K2.m a temperatura ambiente, que es dos veces mayor que el obtenido para muestras sinterizadas sin Ag.

  4. Comparison of Glutamate Turnover in Nerve Terminals and Brain Tissue During [1,6-(13)C2]Glucose Metabolism in Anesthetized Rats.

    Science.gov (United States)

    Patel, Anant B; Lai, James C K; Chowdhury, Golam I M; Rothman, Douglas L; Behar, Kevin L

    2017-01-01

    The (13)C turnover of neurotransmitter amino acids (glutamate, GABA and aspartate) were determined from extracts of forebrain nerve terminals and brain homogenate, and fronto-parietal cortex from anesthetized rats undergoing timed infusions of [1,6-(13)C2]glucose or [2-(13)C]acetate. Nerve terminal (13)C fractional labeling of glutamate and aspartate was lower than those in whole cortical tissue at all times measured (up to 120 min), suggesting either the presence of a constant dilution flux from an unlabeled substrate or an unlabeled (effectively non-communicating on the measurement timescale) glutamate pool in the nerve terminals. Half times of (13)C labeling from [1,6-(13)C2]glucose, as estimated by least squares exponential fitting to the time course data, were longer for nerve terminals (GluC4, 21.8 min; GABAC2 21.0 min) compared to cortical tissue (GluC4, 12.4 min; GABAC2, 14.5 min), except for AspC3, which was similar (26.5 vs. 27.0 min). The slower turnover of glutamate in the nerve terminals (but not GABA) compared to the cortex may reflect selective effects of anesthesia on activity-dependent glucose use, which might be more pronounced in the terminals. The (13)C labeling ratio for glutamate-C4 from [2-(13)C]acetate over that of (13)C-glucose was twice as large in nerve terminals compared to cortex, suggesting that astroglial glutamine under the (13)C glucose infusion was the likely source of much of the nerve terminal dilution. The net replenishment of most of the nerve terminal amino acid pools occurs directly via trafficking of astroglial glutamine.

  5. L-选择素与小鼠肝癌细胞Hepa1-6淋巴道转移的关系%Correlation between L-selectin and lymphatic metastasis potential of mice hepa1-6 cells

    Institute of Scientific and Technical Information of China (English)

    刘芯宇; 张嘉宁

    2013-01-01

    目的 研究L-选择素(L-selectin)与其配体甘露糖受体(mannose receptor,MR)间相互作用与小鼠肝癌细胞Hepa1-6淋巴道转移潜能相关性.方法 构建含有L-selectin的重组载体pcDNA3.1,转染小鼠肝癌细胞 Hepa1-6,筛选稳定表达L-selectin的细胞株.采用免疫组化、RT-PCR、流式细胞、体外黏附试验技术研究小鼠肝癌细胞Hepa1-6中L-selectin的表达及其与肿瘤细胞淋巴道转移潜能的相关性.结果 1重组质粒为目的基因L-selectin.2 Hepa1-6细胞表面有L-selectin表达,阳性率20.2%.3流式细胞仪检测结果表明Hepa1-6细胞表达L-selectin为19.9%.4体外黏附试验结果表明,不具有转移特性的Hepa1-6表达L-selectin后可发生淋巴道转移,L-selectin能与MR结合(P<0.001),介导Hepa1-6的淋巴道转移.结论 Hepa1-6表达目的基因L-selectin后具有淋巴道转移潜能,并能与MR在体外结合,介导肿瘤细胞的淋巴道转移.%Objective To study the correlation between L-selectin and its ligand mannose receptor with mice hepatoma cells Hepal-6 lymphatic metastatic potential. Methods The recombinant plasmids pcDNA3. 1 containing L-selectin was constructed, and transfected mouse liver tumor Hepal-6 cells. The cell strains with a stable expression of L-selectin were screened. Immunochemistry,flow cytometric analysis, RT-PCR and other procedures, in addition to in vitro adhesion assays were performed, to verify the expression of L-selectin on the surface of Hepal -6 cells, and the interactions between L-selectin and tumor cells lymphatic metastasis potential was studied. Results The target gene of recombinant plasmid was L-selectin. L-selectin was expressed on Hepal-6 cell' s surface with the positive rate of 20. 2% . By flow cytometric analysis,the positive expression rate of L-selectin in Hepal-6 cells was 19. 9% . In vitro adhesion assays indicated that this protein was able to bind specifically to MR(P < 0.001) ,and further confirmed that Hepal-6 cells

  6. PDLE: Sustaining Professionalism. Volume 3

    Science.gov (United States)

    Byrd, Patricia, Ed.; Nelson, Gayle, Ed.

    2003-01-01

    This third volume looks at ways that seasoned professionals continue to develop throughout their careers. The text includes descriptive accounts of professionals seeking to enhance their careers while remaining inspired to continue to develop professionally. This volume reveals how personal and professional lives are entwined. It proves that TESOL…

  7. NJP VOLUME 41 NO 2

    African Journals Online (AJOL)

    PROF. EZECHUKWU

    2013-11-24

    Nov 24, 2013 ... sleep. There was also increase in both frequency of uri- nation and volume of urine voided; from1- 2 times to ... phagia, fever, head trauma, chronic cough, weight loss, ... water deprivation at 15Kg. Total volume of urine voided.

  8. Finding related functional neuroimaging volumes

    DEFF Research Database (Denmark)

    Nielsen, Finn Årup; Hansen, Lars Kai

    2004-01-01

    We describe a content-based image retrieval technique for finding related functional neuroimaging experiments by voxelization of sets of stereotactic coordinates in Talairach space, comparing the volumes and reporting related volumes in a sorted list. Voxelization is accomplished by convolving each...

  9. Technological experiment research of superplastic welding of 1.6C%-UHCS/40Cr with preset interlayer%预置中间层的1.6C-UHCS/40Cr超塑性焊接工艺试验

    Institute of Scientific and Technical Information of China (English)

    衡中皓; 张柯柯; 邱然锋; 涂益民; 乔騻; 李臣阳

    2012-01-01

    The experiments without vacuum or protection atmosphere was conducted based on the optimized design of superplastic welding of 1.6C%-UHCS/40Cr with preset interlayer. The results showed that choosing rolled industrial pure iron as an interlayer could improve the 1.6C%-UHCS/40Cr interface's plastic deformation and their weldability effectively. When the initial pressure was 56.6 MPa, welding temperature was 750 XI, initial strain rate was 1.5x10- 4s-1, after 10 min welding, the joint tensile strength could reach 702 MPa, which was 35% larger than without interlayer and equal with the tensile strength of 40Cr base metal after thermodynamic cycle.%基于对预置中间层的1.6C-UHCS/40Cr超塑性焊接工艺方案的优化设计,在非真空及无保护气氛下,进行了超塑性焊接工艺试验.试验结果表明:选用轧制态工业纯铁中间层,能有效改善1.6C-UHCS/40Cr超塑焊界面区的塑性变形能力,提高焊接性.在预压应力56.6 MPa、焊接温度750℃、初始应变速率1.5×10-4/s的条件下,经10 min压接,接头抗拉强度为702 MPa,比不加中间层的接头强度提高35%,达相同热力循环下40Cr母材的抗拉强度.

  10. Discretized Volumes in Numerical Methods

    CERN Document Server

    Antal, Miklós

    2007-01-01

    We present two techniques novel in numerical methods. The first technique compiles the domain of the numerical methods as a discretized volume. Congruent elements are glued together to compile the domain over which the solution of a boundary value problem is sought. We associate a group and a graph to that volume. When the group is symmetry of the boundary value problem under investigation, one can specify the structure of the solution, and find out if there are equispectral volumes of a given type. The second technique uses a complex mapping to transplant the solution from volume to volume and a correction function. Equation for the correction function is given. A simple example demonstrates the feasibility of the suggested method.

  11. Biochemical kinetics in changing volumes.

    Science.gov (United States)

    Pawłowski, Piotr H; Zielenkiewicz, Piotr

    2004-01-01

    The need of taking into account the change of compartment volume when developing chemical kinetics analysis inside the living cell is discussed. Literature models of a single enzymatic Michaelis-Menten process, glycolytic oscillations, and mitotic cyclin oscillations were tested with appropriate theoretical extension in the direction of volume modification allowance. Linear and exponential type of volume increase regimes were compared. Due to the above, in a growing cell damping of the amplitude, phase shift, and time pattern deformation of the metabolic rhythms considered were detected, depending on the volume change character. The performed computer simulations allow us to conclude that evolution of the cell volume can be an essential factor of the chemical kinetics in a growing cell. The phenomenon of additional metabolite oscillations caused by the periodic cell growth and division was theoretically predicted and mathematically described. Also, the hypothesis of the periodized state in the growing cell as the generalization of the steady-state was formulated.

  12. Animation framework using volume visualization

    Science.gov (United States)

    Fang, Wenxuan; Wang, Hongli

    2004-03-01

    As the development of computer graphics, scientific visualization and advanced imaging scanner and sensor technology, high quality animation making of volume data set has been a challenging in industries. A simple animation framework by using current volume visualization techniques is proposed in this paper. The framework consists of two pipelines: one is surface based method by using marching cubes algorithm, the other is volume rendering method by using shear-warp method. The volume visualization results can not only be used as key frame sources in the animation making, but also can be directly used as animation when the volume visualization is in stereoscopic mode. The proposed framework can be applied into fields such as medical education, film-making and archaeology.

  13. Sparse PDF Volumes for Consistent Multi-Resolution Volume Rendering

    KAUST Repository

    Sicat, Ronell Barrera

    2014-12-31

    This paper presents a new multi-resolution volume representation called sparse pdf volumes, which enables consistent multi-resolution volume rendering based on probability density functions (pdfs) of voxel neighborhoods. These pdfs are defined in the 4D domain jointly comprising the 3D volume and its 1D intensity range. Crucially, the computation of sparse pdf volumes exploits data coherence in 4D, resulting in a sparse representation with surprisingly low storage requirements. At run time, we dynamically apply transfer functions to the pdfs using simple and fast convolutions. Whereas standard low-pass filtering and down-sampling incur visible differences between resolution levels, the use of pdfs facilitates consistent results independent of the resolution level used. We describe the efficient out-of-core computation of large-scale sparse pdf volumes, using a novel iterative simplification procedure of a mixture of 4D Gaussians. Finally, our data structure is optimized to facilitate interactive multi-resolution volume rendering on GPUs.

  14. Sparse PDF Volumes for Consistent Multi-Resolution Volume Rendering.

    Science.gov (United States)

    Sicat, Ronell; Krüger, Jens; Möller, Torsten; Hadwiger, Markus

    2014-12-01

    This paper presents a new multi-resolution volume representation called sparse pdf volumes, which enables consistent multi-resolution volume rendering based on probability density functions (pdfs) of voxel neighborhoods. These pdfs are defined in the 4D domain jointly comprising the 3D volume and its 1D intensity range. Crucially, the computation of sparse pdf volumes exploits data coherence in 4D, resulting in a sparse representation with surprisingly low storage requirements. At run time, we dynamically apply transfer functions to the pdfs using simple and fast convolutions. Whereas standard low-pass filtering and down-sampling incur visible differences between resolution levels, the use of pdfs facilitates consistent results independent of the resolution level used. We describe the efficient out-of-core computation of large-scale sparse pdf volumes, using a novel iterative simplification procedure of a mixture of 4D Gaussians. Finally, our data structure is optimized to facilitate interactive multi-resolution volume rendering on GPUs.

  15. High lung volume increases stress failure in pulmonary capillaries

    Science.gov (United States)

    Fu, Z.; Costello, M. L.; Tsukimoto, K.; Prediletto, R.; Elliott, A. R.; Mathieu-Costello, O.; West, J. B.

    1992-01-01

    We previously showed that when pulmonary capillaries in anesthetized rabbits are exposed to a transmural pressure (Ptm) of approximately 40 mmHg, stress failure of the walls occurs with disruption of the capillary endothelium, alveolar epithelium, or sometimes all layers. The present study was designed to test whether stress failure occurred more frequently at high than at low lung volumes for the same Ptm. Lungs of anesthetized rabbits were inflated to a transpulmonary pressure of 20 cmH2O, perfused with autologous blood at 32.5 or 2.5 cmH2O Ptm, and fixed by intravascular perfusion. Samples were examined by both transmission and scanning electron microscopy. The results were compared with those of a previous study in which the lung was inflated to a transpulmonary pressure of 5 cmH2O. There was a large increase in the frequency of stress failure of the capillary walls at the higher lung volume. For example, at 32.5 cmH2O Ptm, the number of endothelial breaks per millimeter cell lining was 7.1 +/- 2.2 at the high lung volume compared with 0.7 +/- 0.4 at the low lung volume. The corresponding values for epithelium were 8.5 +/- 1.6 and 0.9 +/- 0.6. Both differences were significant (P less than 0.05). At 52.5 cmH2O Ptm, the results for endothelium were 20.7 +/- 7.6 (high volume) and 7.1 +/- 2.1 (low volume), and the corresponding results for epithelium were 32.8 +/- 11.9 and 11.4 +/- 3.7. At 32.5 cmH2O Ptm, the thickness of the blood-gas barrier was greater at the higher lung volume, consistent with the development of more interstitial edema. Ballooning of the epithelium caused by accumulation of edema fluid between the epithelial cell and its basement membrane was seen at 32.5 and 52.5 cmH2O Ptm. At high lung volume, the breaks tended to be narrower and fewer were oriented perpendicular to the axis of the pulmonary capillaries than at low lung volumes. Transmission and scanning electron microscopy measurements agreed well. Our findings provide a physiological

  16. Microstructure and property of 1.6% C-UHCS/40Cr joint after superplastic welding via interlayer%具有中间夹层的1.6%C-UHCS/40Cr超塑性焊接接头组织和性能

    Institute of Scientific and Technical Information of China (English)

    衡中皓; 张柯柯; 乔騻; 张占领; 张晓娇; 涂益民

    2012-01-01

    采用工业纯铁中间层,在非真空、无保护气氛条件下,进行1.6%C-UHCS/40Cr的超塑性焊接试验.试验结果表明,采用工业纯铁中间层能提高1.6%C-UHCS/40Cr超塑性焊接接头界面塑性变形能力,促进扩散,改善接头区组织性能.在预压应力56.6 MPa、焊接温度780℃、初始应变速率1.5×10-4/S的条件下,经15 min压接,接头强度可达560 MPa,比不加中间层的超塑性焊接接头强度提高了46%.%Since the superplastic welding of quenched 40Cr and thermo-mechanical processed 1.6% ultrahigh carbon steel(1.6%C-UHCS) is of great possibility.Conduct the superplastic welding experiment of 1.6%C-UHCS/40Cr via industrial pure iron interlayer without protection of vacuum or atmosphere.By means of modern physical and chemical detection means, analyze the joint mechanical performance,microstructure and defects of superplastic welded joints with and without interlayer.Study the improving effect of industrial pure iron on the joint as the interlayer.The results show that with interlayer,the joint's plastic deformation capacity was improved,diffusion was promoted and its joint microstructure was improved.When the prepressing stress is 56.6 Mpa.the welding temperature is 780 ℃the initial strain rate is 1.5×10-4/s,after 15 min welding,the joint strength can reach 560 Mpa,which is 46% more than that without interlayer.

  17. Strontium fructose 1,6-diphosphate prevents bone loss in a rat model of postmenopausal osteoporosis via the OPG/RANKL/RANK pathway

    Institute of Scientific and Technical Information of China (English)

    Bo MA; Qi ZHANG; Di WU; Yong-lu WANG; Ying-ying HU; Yan-ping CHENG; Zhen-dong YANG; Ya-ya ZHENG; Han-jie YING

    2012-01-01

    Aim:To evaluate the protective effects of strontium fructose 1,6-diphosphate (FDP-Sr),a novel strontium salt that combined fructose 1,6-diphosphate (FDP)with strontium,on bone in an ovariectomy-induced model of bone loss.Methods:Eighty female Sprague-Dawley rats were ovariectomized (OVX)or sham-operated.Three months later,the rats were assigned to six groups (10 for each):sham-operated,OVX control,OVX+FDP-Sr (110,220,or 440 mg/kg),or OVX+strontium ranelate (SR,180 mg/kg).Drugs were administered orally for 3 months.When the treatment was terminated,the following parameters were assessed:bone mineral density (BMD),the biomechanical properties of the femur and lumbar vertebrae,trabecular histomorphology,serum phosphorus,calcium,bone-specific alkaline phosphatase (B-ALP),tartrate-resistant acid phosphatase 5b (TRACP5b),N-telopeptide of type I collagen (NTx)and a series of markers for oxidative stress.Receptor activator of NF-kB ligand (RANKL)and osteoprotegerin (OPG)levels in serum were measured using ELISA and their gene expression levels in the bone were measured using R-T PCR.Results:Treatment with FDP-Sr (220 or 440 mg/kg)or SR (180 mg/kg)significantly increased the BMD and improved the bone microarchitecture and bone strength in OVX rats.The treatments also decreased in the levels of H202 and MDA,restored the CAT level in serum and bone marrow,increased the serum B-ALP and decreased NTx and TRACP 5b in OVX rats.Treatment with FDP-Sr decreased the RANKL level,and increased the OPG level in serum in a dose-dependent manner.It also significantly down-regulated the RANKL expression and ul-regulated OPG expression in bone marrow.Conclusion:FDP-Sr may be an effectve treatment for postmenopausal osteoporosis that acts,in part,via a decrease in osteoclastogenesis through the OPG\\RANKL\\RANK pathway.

  18. Using GDAL to Convert NetCDF 4 CF 1.6 to GeoTIFF: Interoperability Problems and Solutions for Data Providers and Distributors

    Science.gov (United States)

    Haran, T. M.; Brodzik, M. J.; Nordgren, B.; Estilow, T.; Scott, D. J.

    2015-12-01

    An increasing number of new Earth science datasets are being producedby data providers in self-describing, machine-independent file formatsincluding Hierarchical Data Format version 5 (HDF5) and NetworkCommon Data Form version 4 (netCDF-4). Furthermore data providers maybe producing netCDF-4 files that follow the conventions for Climateand Forecast metadata version 1.6 (CF 1.6) which, for datasets mappedto a projected raster grid covering all or a portion of the earth,includes the Coordinate Reference System (CRS) used to define howlatitude and longitude are mapped to grid coordinates, i.e. columnsand rows, and vice versa. One problem that users may encounter is thattheir preferred visualization and analysis tool may not yet includesupport for one of these newer formats. Moreover, data distributorssuch as NASA's NSIDC DAAC may not yet include support for on-the-flyconversion of data files for all data sets produced in a new format toa preferred older distributed format.There do exist open source solutions to this dilemma in the form ofsoftware packages that can translate files in one of the new formatsto one of the preferred formats. However these software packagesrequire that the file to be translated conform to the specificationsof its respective format. Although an online CF-Convention compliancechecker is available from cfconventions.org, a recent NSIDC userservices incident described here in detail involved an NSIDC-supporteddata set that passed the (then current) CF Checker Version 2.0.6, butwas in fact lacking two variables necessary for conformance. Thisproblem was not detected until GDAL, a software package which reliedon the missing variables, was employed by a user in an attempt totranslate the data into a different file format, namely GeoTIFF.This incident indicates that testing a candidate data product with oneor more software products written to accept the advertised conventionsis proposed as a practice which improves interoperability

  19. Immune responses induced by Hepa1-6 hepotoma cell after over-expression SEA and CD80 in vitro%SEA-CD80基因过表达Hepa1-6肝癌细胞体外诱导的免疫学效应

    Institute of Scientific and Technical Information of China (English)

    司少艳; 史亮; 刘俊丽; 宋淑军; 景青萍; 吴继华; 张建中

    2011-01-01

    This study is aimed to determine whether Hepal-6 hepotoma cells could induce antitumor immune response after over-expression Staphylococcus enterotoxin A (SEA) and CD80. Mouse spleen lymphocytes were cocultured with hepal-6 cells infected with empty adenoviruse vector Ad (empty) or the recombinant aden-oviruses of Ad-MMRE-mTERT-B7, Ad-MMRE-mTERT-SEA or Ad-MMRE-mTERT-BIS. Lymphocyte proliferation was assayed by BrdU incorporation using a commercial kit, flow cytometric analysis was used to detect proliferation of T Lymphocyte subgroups. The production of IL-2, TNF-a and IFN-γ was tested by enzyme-linked immunosorbent assay, and antitumor activity of CTL was determined by lactate dehydrogenase release assay. Compared with Hepa1-6 infected with empty adenovirus or without infection, Hepal-6 infected with recombinant adenoviruses could significantly induce proliferatioin of spleen lymphocytes and T Lymphocyte subgroups, enhance IL-2, TNF-a and IFN-γ production and CTL killing activities. The antitumor response was significantly stronger in Hepal-6 cells after dual-gene over-expression than that in Hepal-6 cells after single-gene over-expression. The results indicate that SEA and CD80 expressed on the membrane of Hepal-6 cells by infection with recombinant adenovirus has immune activities. This study provides some experimental evidence for further tumor immune genetherapy with the recombinant adenovirus.%观察经SEA和CD80重组腺病毒感染Hepa1-6肝癌细胞后体外能否诱导抗肿瘤免疫学反应.方法 Hepa1-6细胞分别经空载体腺病毒Ad(空)和重组腺病毒Ad-MMRE-mTERT-B7、Ad-MMRE-mTERT-SEA、Ad-MMRE -mTERT-BIS感染后,和小鼠脾淋巴细胞共培养,然后采用Brdu酶联免疫法(ELISA)检测淋巴细胞增殖;流式细胞术检测T淋巴细胞亚群增殖;ELISA法检测细胞因子IL-2、TNF-α和IFN-γ的产生;LDH释放法检测CTLs对Hepa1-6的杀伤作用.结果与感染空载体腺病毒Ad(空)和未感染Hepa 1-6细胞相比,

  20. Minivoids in the Local Volume

    CERN Document Server

    Tikhonov, A V

    2006-01-01

    We consider a sphere of 7.5 Mpc radius, which contains 355 galaxies with accurately measured distances, to detect the nearest empty volumes. Using a simple void detection algorithm, we found six large (mini)voids in Aquila, Eridanus, Leo, Vela, Cepheus and Octans, each of more than 30 Mpc^3. Besides them, 24 middle-size "bubbles" of more than 5 Mpc^3 volume are detected, as well as 52 small "pores". The six largest minivoids occupy 58% of the considered volume. Addition of the bubbles and pores to them increases the total empty volume up to 75% and 81%, respectively. The detected local voids look like oblong potatoes with typical axial ratios b/a = 0.75 and c/a = 0.62 (in the triaxial ellipsoide approximation). Being arranged by the size of their volume, local voids follow power law of volumes-rankes dependence. A correlation Gamma-function of the Local Volume galaxies follows a power low with a formally calculated fractal dimension D = 1.5. We found that galaxies surrounding the local minivoids do not differ...

  1. FLIS Procedures Manual. Document Identifier Code Input/Output Formats (Fixed Length). Volume 8.

    Science.gov (United States)

    2007-11-02

    4100.39-M DEFENSE LOGISTICS SERVICES CENTER Volume 8 74 WASHINGTON AVE N BATTLE CREEK MI 49017-3084 DLSC- VPH 1 April 1997 FOREWORD This is one of the...volume 1, chapter 1.6, or administrative comments and inquiries may be directed to DLSC- VPH . Service/Agency distribution is handled through...changes to DLSC- VPH . Content changes appearing on these pages are entered in bold-faced italic type. Comments or questions may be directed to DLSC- VPH . This

  2. The first of a new generation of diesel engines from General Motors - the efficient and powerful 1.6 liter Euro6 midsize diesel engine

    Energy Technology Data Exchange (ETDEWEB)

    Boretto, Gianmarco; Golisano, Roberto; Scotti, Michele; Antonioli, Pierpaolo; Frank, Richard M.; Rovatti, Giovanni [General Motors Powertrain Europe s.r.l., Turin (Italy); Wesslau, Markus [Adam Opel AG, Ruesselsheim (Germany)

    2013-08-01

    The major challenge the automotive industry will face in the next decade is undoubtedly the reduction of CO{sub 2} emissions. Conventional powertrains with internal combustion engines will still play a predominant role: in particular, the diesel engine will be a major contributor to the solution thanks to its intrinsic high thermodynamic efficiency and low-end torque which is a key enabler for downsizing engine displacement and downspeeding. In this context, General Motors has developed an entirely new 1.6 liter four-cylinder Midsize Diesel Engine (MDE), the first of a new generation of efficient and powerful diesel engines. Its development has been focused on the achievement of high power and torque density, superior fuel efficiency and state-of-the-art noise and vibration, while meeting the Euro6 emission standard in a wide range of B-, C- and D-segment vehicles, utilizing different exhaust aftertreatment solutions. The key technical features of the base engine, combustion system and emission reduction technologies, together with the innovative engine control unit, are described in this paper. (orig.)

  3. Measurements of $ep \\to e'\\pi^+n$ at 1.6

    CERN Document Server

    Park, K; Burkert, V D

    2014-01-01

    Differential cross sections of the exclusive process $e p \\to e^\\prime \\pi^+ n$ were measured with good precision in the range of the photon virtuality $Q^2 = 1.8 - 4.5$ GeV$^2$, and the invariant mass range of the $\\pi^+ n$ final state $W = 1.6 - 2.0$ GeV using the CEBAF Large Acceptance Spectrometer. Data were collected with nearly complete coverage in the azimuthal and polar angles of the $n\\pi^+$ center-of-mass system. More than 37,000 cross section points were measured. The contributions of the isospin $I = {1\\over 2}$ resonances $N(1675){5\\over 2}^-$, $N(1680){5\\over 2}^+$ and $N(1710){1\\over 2}^+$ were extracted at different values of $Q^2$ using a single-channel, energy-dependent resonance amplitude analysis. Two different approaches, the unitary isobar model and the fixed-$t$ dispersion relations, were employed in the analysis. We observe significant strength of the $N(1675){5\\over 2}^-$ in the $A_{1/2}$ amplitude, which is in strong disagreement with quark models that predict both transverse amplitu...

  4. Externally applied electric fields up to 1.6 × 10(5) V/m do not affect the homogeneous nucleation of ice in supercooled water.

    Science.gov (United States)

    Stan, Claudiu A; Tang, Sindy K Y; Bishop, Kyle J M; Whitesides, George M

    2011-02-10

    The freezing of water can initiate at electrically conducting electrodes kept at a high electric potential or at charged electrically insulating surfaces. The microscopic mechanisms of these phenomena are unknown, but they must involve interactions between water molecules and electric fields. This paper investigates the effect of uniform electric fields on the homogeneous nucleation of ice in supercooled water. Electric fields were applied across drops of water immersed in a perfluorinated liquid using a parallel-plate capacitor; the drops traveled in a microchannel and were supercooled until they froze due to the homogeneous nucleation of ice. The distribution of freezing temperatures of drops depended on the rate of nucleation of ice, and the sensitivity of measurements allowed detection of changes by a factor of 1.5 in the rate of nucleation. Sinusoidal alternation of the electric field at frequencies from 3 to 100 kHz prevented free ions present in water from screening the electric field in the bulk of drops. Uniform electric fields in water with amplitudes up to (1.6 ± 0.4) × 10(5) V/m neither enhanced nor suppressed the homogeneous nucleation of ice. Estimations based on thermodynamic models suggest that fields in the range of 10(7)-10(8) V/m might cause an observable increase in the rate of nucleation.

  5. Longitudinal and lateral static stability and control characteristics of a 1/6-scale model of a remotely piloted research vehicle with a supercritical wing

    Science.gov (United States)

    Byrdsong, T. A.; Hallissy, J. B.

    1979-01-01

    An investigation was conducted in the Langley 8-foot transonic pressure tunnel to determine the longitudinal and lateral-directional static stability and control characteristics of a 1/6-scale force model of a remotely piloted research vehicle. The model was equipped with a supercritical wing and employed elevons for pitch and roll control. Test conditions were as follows: Reynolds number of about 6.6 x 10 to the 6th power per meter, variations of sideslip from -6 deg to 6 deg, elevon deflection angle (symmetrically and asymmetrically) from -9 deg to 3 deg, and rudder deflection angle from 0 deg to -10 deg. The model was longitudinally statically stable at angles of attack up to about 7 deg, which is significantly greater than the angle of attack for the cruise condition (approximately 4 deg). In the range of test Mach numbers, the model was directionally stable and had positive effective dihedral, sufficient pitch control, and positive effectiveness of roll and yaw control.

  6. Effects of fructose-1,6-diphosphate on concentration of calcium and activities of sarcoplosnic Ca2+-ATPase in cardiomyocytes of Adriamycin-treated rats

    Institute of Scientific and Technical Information of China (English)

    CAI Wei; CHEN Jun-zhu; RUAN Li-ming; WANG Yi-na

    2005-01-01

    Objective: To observe the effects of fructose-1,6-diphosphate (FDP) on serum levels of cardiac troponin I (cTnI) and creatine kinase-MB (CK-MB), as well as the concentration of calcium in cardiomyocytes (Myo[Ca2+]) and activity of sarcoplosnic Ca2+-ATPase (SRCa2+-ATPase) in Adriamycin (ADR)-treated rats. Methods: Rats were intraperitoneally injected with ADR (2.5mg/kg every other day for 6 times) and then with different dosages of FDP (every other day for twenty-one times). Bi-antibodies sandwich Enzyme linked immune absorption assay (ELISA) was performed to detect serum level of cTnI. CK-MB was detected by monoclonal antibody, Myo[Ca2+] was detected by fluorescent spectrophotometry and the activity of SRCa2+-ATPase was detected by inorganic phosphate method. Results: FDP (300, 600, 1200 mg/kg) significantly reduced the serum levels of cTnI and CK-MB, while at the same time decreased calcium concentration and increased SRCa2+-ATPase activity in cardiomyocytes of ADR-treated rats (P<0.01). Conclusions: FDP might alleviate the cardiotoxic effects induced by ADR through decreasing calcium level as well as increasing SRCa2+-ATPase activity in cardiomyocytes.

  7. KEPLER-21b: A 1.6 R{sub Earth} PLANET TRANSITING THE BRIGHT OSCILLATING F SUBGIANT STAR HD 179070

    Energy Technology Data Exchange (ETDEWEB)

    Howell, Steve B. [National Optical Astronomy Observatory, Tucson, AZ 85719 (United States); Rowe, Jason F.; Bryson, Stephen T. [NASA Ames Research Center, Moffett Field, CA 94035 (United States); Quinn, Samuel N. [Harvard-Smithsonian Center for Astrophysics, Cambridge, MA 02138 (United States); Marcy, Geoffrey W.; Isaacson, Howard [Department of Astronomy, University of California, Berkeley, CA 94720 (United States); Ciardi, David R. [NASA Exoplanet Science Institute/Caltech, Pasadena, CA 91125 (United States); Chaplin, William J.; Elsworth, Yvonne [School of Physics and Astronomy, University of Birmingham, Edgbaston, Birmingham B15 2TT (United Kingdom); Metcalfe, Travis S. [High Altitude Observatory and Scientific Computing Division, National Center for Atmospheric Research, Boulder, CO 80307 (United States); Monteiro, Mario J. P. F. G. [Centro de Astrofisica, Universidade do Porto, Rua das Estrelas, 4150-762 Porto (Portugal); Appourchaux, Thierry [Institut d' Astrophysique Spatiale, Universite Paris XI-CNRS (UMR8617), Batiment 121, 91405 Orsay Cedex (France); Basu, Sarbani [Department of Astronomy, Yale University, New Haven, CT 06520-8101 (United States); Creevey, Orlagh L. [Departamento de Astrofisica, Universidad de La Laguna, E-38206 La Laguna, Tenerife (Spain); Gilliland, Ronald L. [Space Telescope Science Institute, Baltimore, MD 21218 (United States); Quirion, Pierre-Olivier [Canadian Space Agency, 6767 Boulevard de l' Aeroport, Saint-Hubert, QC, J3Y 8Y9 (Canada); Stello, Denis [Sydney Institute for Astronomy (SIfA), School of Physics, University of Sydney, NSW 2006 (Australia); Kjeldsen, Hans; Christensen-Dalsgaard, Joergen [Department of Physics and Astronomy, Aarhus University, DK-8000 Aarhus C (Denmark); Garcia, Rafael A. [Laboratoire AIM, CEA/DSM-CNRS-Universite Paris Diderot-IRFU/SAp, 91191 Gif-sur-Yvette Cedex (France); and others

    2012-02-20

    We present Kepler observations of the bright (V = 8.3), oscillating star HD 179070. The observations show transit-like events which reveal that the star is orbited every 2.8 days by a small, 1.6 R{sub Earth} object. Seismic studies of HD 179070 using short cadence Kepler observations show that HD 179070 has a frequency-power spectrum consistent with solar-like oscillations that are acoustic p-modes. Asteroseismic analysis provides robust values for the mass and radius of HD 179070, 1.34 {+-} 0.06 M{sub Sun} and 1.86 {+-} 0.04 R{sub Sun }, respectively, as well as yielding an age of 2.84 {+-} 0.34 Gyr for this F5 subgiant. Together with ground-based follow-up observations, analysis of the Kepler light curves and image data, and blend scenario models, we conservatively show at the >99.7% confidence level (3{sigma}) that the transit event is caused by a 1.64 {+-} 0.04 R{sub Earth} exoplanet in a 2.785755 {+-} 0.000032 day orbit. The exoplanet is only 0.04 AU away from the star and our spectroscopic observations provide an upper limit to its mass of {approx}10 M{sub Earth} (2{sigma}). HD 179070 is the brightest exoplanet host star yet discovered by Kepler.

  8. C6 Coefficients and Dipole Polarizabilities for All Atoms and Many Ions in Rows 1-6 of the Periodic Table.

    Science.gov (United States)

    Gould, Tim; Bučko, Tomáš

    2016-08-09

    Using time-dependent density functional theory (TDDFT) with exchange kernels, we calculate and test imaginary frequency-dependent dipole polarizabilities for all atoms and many ions in rows 1-6 of the periodic table. These are then integrated over frequency to produce C6 coefficients. Results are presented under different models: straight TDDFT calculations using two different kernels; "benchmark" TDDFT calculations corrected by more accurate quantum chemical and experimental data; and "benchmark" TDDFT with frozen orbital anions. Parametrizations are presented for 411+ atoms and ions, allowing results to be easily used by other researchers. A curious relationship, C6,XY ∝ [αX(0)αY(0)](0.73), is found between C6 coefficients and static polarizabilities α(0). The relationship C6,XY = 2C6,XC6,Y/[(αX/αY)C6,Y + (αY/αX)C6,X] is tested and found to work well (30% errors) in a small fraction of cases.

  9. Functional characterization of the gene FoOCH1 encoding a putative α-1,6-mannosyltransferase in Fusarium oxysporum f. sp. cubense.

    Science.gov (United States)

    Li, Min-Hui; Xie, Xiao-Ling; Lin, Xian-Feng; Shi, Jin-Xiu; Ding, Zhao-Jian; Ling, Jin-Feng; Xi, Ping-Gen; Zhou, Jia-Nuan; Leng, Yueqiang; Zhong, Shaobin; Jiang, Zi-De

    2014-04-01

    Fusarium oxysporum f. sp. cubense (FOC) is the causal agent of banana Fusarium wilt and has become one of the most destructive pathogens threatening the banana production worldwide. However, few genes related to morphogenesis and pathogenicity of this fungal pathogen have been functionally characterized. In this study, we identified and characterized the disrupted gene in a T-DNA insertional mutant (L953) of FOC with significantly reduced virulence on banana plants. The gene disrupted by T-DNA insertion in L953 harbors an open reading frame, which encodes a protein with homology to α-1,6-mannosyltransferase (OCH1) in fungi. The deletion mutants (ΔFoOCH1) of the OCH1 orthologue (FoOCH1) in FOC were impaired in fungal growth, exhibited brighter staining with fluorescein isothiocyanate (FITC)-Concanavalin A, had less cell wall proteins and secreted more proteins into liquid media than the wild type. Furthermore, the mutation or deletion of FoOCH1 led to loss of ability to penetrate cellophane membrane and decline in hyphal attachment and colonization as well as virulence to the banana host. The mutant phenotypes were fully restored by complementation with the wild type FoOCH1 gene. Our data provide a first evidence for the critical role of FoOCH1 in maintenance of cell wall integrity and virulence of F. oxysporum f. sp. cubense. Copyright © 2014 Elsevier Inc. All rights reserved.

  10. Enzymatic synthesis of fructose 1,6-diphosphate with ATP regeneration in a batch reactor and a semibatch reactor using purified enzymes of Bacillus stearothermophilus.

    Science.gov (United States)

    Widjaja, A; Shiroshima, M; Yasuda, M; Ogino, H; Nakajima, H; Ishikawa, H

    1999-01-01

    The enzymatic synthesis of fructose 1,6-diphosphate (FDP), an important glycolytic intermediate whose applications in the field of medicine have generated a great deal of interest, was performed in a batch reactor and a semibatch reactor. Using the batch reactor, FDP was first synthesized from glucose by three enzymatic reactions and the ATP consumed was regenerated simultaneously using conjugated enzymes, all of which were purified from crude cell extract of thermophilic Bacillus stearothermophilus. The results of the experiments performed using several enzyme concentrations suggest the existence of an optimum concentration for each enzyme at which the maximum FDP yield can be attained. Since the thermal decomposition of acetyl phosphate reduced the yield of FDP in the batch reactor, the use of a semibatch reactor in which acetyl phosphate was fed continuously was examined. The yield of FDP was improved but the time required to complete the reaction was longer, resulting in a lower productivity of FDP. The yields observed in the two reactors using various enzyme and substrate concentrations were in good agreement with the theoretical predictions calculated based on differential equations derived for the system using the rate equations and the kinetic parameters determined previously. This means that these equations can be used for the analysis of the experimental results as well as for determining the optimum experimental conditions.

  11. Crystal structure of FhuD at 1.6 Å resolution: a ferrichrome-binding protein from the animal and human pathogen Staphylococcus pseudintermedius.

    Science.gov (United States)

    Abate, Francesca; Cozzi, Roberta; Maritan, Martina; Lo Surdo, Paola; Maione, Domenico; Malito, Enrico; Bottomley, Matthew James

    2016-03-01

    Staphylococcus pseudintermedius is a leading cause of disease in dogs, and zoonosis causes human infections. Methicillin-resistant S. pseudintermedius strains are emerging, resembling the global health threat of S. aureus. Therefore, it is increasingly important to characterize potential targets for intervention against S. pseudintermedius. Here, FhuD, an S. pseudintermedius surface lipoprotein implicated in iron uptake, was characterized. It was found that FhuD bound ferrichrome in an iron-dependent manner, which increased the thermostability of FhuD by >15 °C. The crystal structure of ferrichrome-free FhuD was determined via molecular replacement at 1.6 Å resolution. FhuD exhibits the class III solute-binding protein (SBP) fold, with a ligand-binding cavity between the N- and C-terminal lobes, which is here occupied by a PEG molecule. The two lobes of FhuD were oriented in a closed conformation. These results provide the first detailed structural characterization of FhuD, a potential therapeutic target of S. pseudintermedius.

  12. AGN triggering in the infall regions of distant X-ray luminous galaxy clusters at 0.9 < z <~ 1.6

    CERN Document Server

    Fassbender, R; Nastasi, A

    2012-01-01

    Observational constraints on the average radial distribution profile of AGN in distant galaxy clusters can provide important clues on the triggering mechanisms of AGN activity in dense environments and are essential for a completeness evaluation of cluster selection techniques in the X-ray and mm-wavebands. The aim of this work is a statistical study with XMM-Newton of the presence and distribution of X-ray AGN in the large-scale structure environments of 22 X-ray luminous galaxy clusters in the redshift range 0.9 < z \\lesssim 1.6 compiled by the XMM-Newton Distant Cluster Project (XDCP). To this end, the X-ray point source lists from detections in the soft-band (0.35-2.4 keV) and full-band (0.3-7.5 keV) were stacked in cluster-centric coordinates and compared to average background number counts extracted from three independent control fields in the same observations. A significant full-band (soft-band) excess of \\sim78 (67) X-ray point sources is found in the cluster fields within an angular distance of 8...

  13. Iodinin (1,6-Dihydroxyphenazine 5,10-Dioxide from Streptosporangium sp. Induces Apoptosis Selectively in Myeloid Leukemia Cell Lines and Patient Cells

    Directory of Open Access Journals (Sweden)

    Lars Herfindal

    2013-01-01

    Full Text Available Despite recent improvement in therapy, acute myeloid leukemia (AML is still associated with high lethality. In the presented study, we analyzed the bioactive compound iodinin (1,6-dihydroxyphenazine 5,10-dioxide from a marine actinomycetes bacterium for the ability to induce cell death in a range of cell types. Iodinin showed selective toxicity to AML and acute promyelocytic (APL leukemia cells, with EC50 values for cell death up to 40 times lower for leukemia cells when compared with normal cells. Iodinin also successfully induced cell death in patient-derived leukemia cells or cell lines with features associated with poor prognostic such as FLT3 internal tandem duplications or mutated/deficient p53. The cell death had typical apoptotic morphology, and activation of apoptotic signaling proteins like caspase-3. Molecular modeling suggested that iodinin could intercalate between bases in the DNA in a way similar to the anti-cancer drug daunorubicin (DNR, causing DNA-strand breaks. Iodinin induced apoptosis in several therapy-resistant AML-patient blasts, but to a low degree in peripheral blood leukocytes, and in contrast to DNR, not in rat cardiomyoblasts. The low activity towards normal cell types that are usually affected by anti-leukemia therapy suggests that iodinin and related compounds represent promising structures in the development of anti-cancer therapy.

  14. Loss of α1,6-fucosyltransferase suppressed liver regeneration: implication of core fucose in the regulation of growth factor receptor-mediated cellular signaling.

    Science.gov (United States)

    Wang, Yuqin; Fukuda, Tomohiko; Isaji, Tomoya; Lu, Jishun; Gu, Wei; Lee, Ho-Hsun; Ohkubo, Yasuhito; Kamada, Yoshihiro; Taniguchi, Naoyuki; Miyoshi, Eiji; Gu, Jianguo

    2015-02-05

    Core fucosylation is an important post-translational modification, which is catalyzed by α1,6-fucosyltransferase (Fut8). Increased expression of Fut8 has been shown in diverse carcinomas including hepatocarcinoma. In this study, we investigated the role of Fut8 expression in liver regeneration by using the 70% partial hepatectomy (PH) model, and found that Fut8 is also critical for the regeneration of liver. Interestingly, we show that the Fut8 activities were significantly increased in the beginning of PH (~4d), but returned to the basal level in the late stage of PH. Lacking Fut8 led to delayed liver recovery in mice. This retardation mainly resulted from suppressed hepatocyte proliferation, as supported not only by a decreased phosphorylation level of epidermal growth factor (EGF) receptor and hepatocyte growth factor (HGF) receptor in the liver of Fut8(-/-) mice in vivo, but by the reduced response to exogenous EGF and HGF of the primary hepatocytes isolated from the Fut8(-/-) mice. Furthermore, an administration of L-fucose, which can increase GDP-fucose synthesis through a salvage pathway, significantly rescued the delayed liver regeneration of Fut8(+/-) mice. Overall, our study provides the first direct evidence for the involvement of Fut8 in liver regeneration.

  15. Observation of vertcal CO2 concentration profiles in the lower-atmosphere using a compact direct detection 1.6 μm DIAL

    Science.gov (United States)

    Nagasawa, C.; Shibata, Y.; Abo, M.

    2015-12-01

    Knowledge of present carbon sources and sinks including their spatial profile and their variation in time is one of the essential informations for predicting future CO2 atmospheric concentration levels. Moewover, for the detailed analysis of forest carbon dynamics and CO2 fluxes of urban area, the CO2 concentration measurement techniques with high spatial and temporal resolution are required in the lower atmosphere. A differential absorption lidar (DIAL) is expected to measure atmospheric CO2 concentration profiles in the atmospheric boundary layer from a ground platform. We have succeeded to develop a compact direct detection 1.6 μm DIAL system for measuring CO2 concentration profiles in the lower atmosphere. This DIAL system consists of the optical parametric generator (OPG) transmitter that excited by the LD pumped Nd:YAG laser with high repetition rate and the receiving optics that included the near-infrared photomultiplier tube operating at the analog mode and the 25 cm telescope. We have succeeded in observing the daytime temporal change of vertical CO2 concentration profiles for the range from 0.25 to 2.5 km with integration time of 30 minutes and range resolution of 300 m. This compact direct detection CO2 DIAL is usefull for the estimation of CO2 flux. This work was financially supported by the System Development Program for Advanced Measurement and Analysis of the Japan Science and Technology Agency.

  16. Microbial mat-induced sedimentary structures in siliciclastic sediments: Examples from the 1.6 Ga Chorhat Sandstone, Vindhyan Supergroup, M.P., India

    Indian Academy of Sciences (India)

    Subir Sarkar; Santanu Banerjee; Pradip Samanta; Silambuchelvan Jeevankumar

    2006-02-01

    This paper addresses macroscopic signatures of microbial mat-related structures within the 1.6 Ga-old Chorhat Sandstone of the Semri Group –the basal stratigraphic unit of the Vindhyan succession in Son valley.The Chorhat Sandstone broadly represents a prograding succession of three depositional facies ranging from shallow shelf to coastal margin with aeolian sandsheet.The mat-mediated structures were generated because of plastic or brittle deformation of sand,turned cohesive and even thixotropic because of microbial mat growth.Mat growth also favoured abundant preservation of structures that usually have low preservation potential. Prolific growth of microbial mat in the subtidal to intertidal zone of the Chorhat sea was facilitated due to lack of grazing and burrowing activities of organisms in the Precambrian.It further indicates low rate of sedimentation between the storms,as also attested by frequent superposition of storm-beds,even near the storm wave base.It also reduces erosion and that,in turn,would imply low sediment concentration in flows leading to development of bedforms that are likely to be smaller in size and isolated from each other in a single train in contrast to those that form in mat-free sands.

  17. Sensory threshold of 1,1,6-trimethyl-1,2-dihydronaphthalene (TDN) and concentrations in young Riesling and non-Riesling wines.

    Science.gov (United States)

    Sacks, Gavin L; Gates, Matthew J; Ferry, Francois X; Lavin, Edward H; Kurtz, Anne J; Acree, Terry E

    2012-03-28

    1,1,6-Trimethyl-1,2-dihydronaphthalene (TDN) is well-known to contribute "petrol" aromas to aged Riesling wines, but its prevalence and contribution to young Riesling or non-Riesling wines is not well understood. TDN concentrations were measured in 1-3-year-old varietal wines produced from Cabernet franc (n = 14 wines), Chardonnay (17), Cabernet Sauvignon (4), Gewurztraminer (4), Merlot (9), Pinot gris (6), Pinot noir (9), Riesling (28), or Sauvignon blanc (6). TDN concentrations in the Riesling wines, 6.4 ± 3.8 μg/L, were significantly higher than in all other varietals, 1.3 ± 0.8 μg/L. The odor detection thresholds for TDN were then determined in both model wine and a neutral white wine. Group sensory thresholds were found to be the same in both matrices, 2 μg/L, indicating little masking of TDN due to the odorants in the neutral white. The TDN sensory threshold was a factor of 10 below the previously reported odor threshold. On the basis of this revised threshold, 27 of 28 Riesling wines had suprathreshold TDN, whereas only 7 of 69 non-Riesling wines had suprathreshold TDN. The monoterpenes linalool and geraniol were also measured in the Riesling wines, and odor activity values (OAVs) were calculated for the monoterpenes and TDN. The OAV for TDN was higher than for the monoterpenes in 25 of 28 Riesling wines.

  18. Temporal Evolution of Chromospheric Evaporation: Case Studies of the M1.1 Flare on 2014 September 6 and X1.6 Flare on 2014 September 10

    Science.gov (United States)

    Tian, Hui; Young, Peter R.; Reeves, Katharine K.; Chen, Bin; Liu, Wei; McKillop, Sean

    2015-10-01

    With observations from the Interface Region Imaging Spectrograph, we track the complete evolution of ˜11 MK evaporation flows in an M1.1 flare on 2014 September 6 and an X1.6 flare on 2014 September 10. These hot flows, as indicated by the blueshifted Fe xxi 1354.08 Å line, evolve smoothly with a velocity decreasing exponentially from ˜200 km s-1 to almost stationary within a few minutes. We find a good correlation between the flow velocity and energy deposition rate as represented by the hard X-ray flux observed with the Reuven Ramaty High Energy Solar Spectroscopic Imager, or time derivative of the soft X-ray flux observed with the Geostationary Operational Environmental Satellites and the HINODE X-ray Telescope, which is in general agreement with models of nonthermal electron heating. The maximum blueshift of Fe xxi appears approximately at the same time as or slightly after the impulsive enhancement of the ultraviolet continuum and the Mg ii 2798.8 Å line emission, demonstrating that the evaporation flow is closely related to heating of the lower chromosphere. Finally, while the hot Fe xxi 1354.08 Å line is entirely blueshifted with no obvious rest component, cool chromospheric and transition region lines like Si iv 1402.77 Å are often not entirely redshifted but just reveal an obvious red wing enhancement at the ribbons, suggesting that the speed of chromospheric condensation might be larger than previously thought.

  19. Fructose-1,6-bisphosphatase: Genetic and physical mapping to human chromosome 9q22.3 and evaluation in non-insulin-dependent diabetes mellitus

    Energy Technology Data Exchange (ETDEWEB)

    Rothschild, C.B.; Akots, G.; Roh, B. [Wake Forest Univ., Winston-Salem, NC (United States)] [and others

    1995-09-01

    PCR primers specific to the human liver fructose-1,6-bisphosphatase (FBP) gene were designed and used to isolate a cosmid clone. Physical mapping of the FBP cosmid by FISH, and genetic mapping of an associated GA repeat polymorphism (PIC = 0.35), located the liver FBP gene to chromosome 9q22.3 with no recombination between FBP and the index markers D9S196 (Z{sub max} = 13.2), D9S280 (Z{sub max} = 11.7), D9S287 (Z{sub max} = 15.6), and D9S176 (Z{sub max} = 14.4). Amplification using FBP exon-specific primers with a YAC contig from this region of chromosome 9 further refined the placement of FBP genomic sequences to an approximately 1.7-cM region flanked by D9S280 and D9S287, near the gene for Fanconi anemia group C. Precise localization of the FBP gene enabled evaluation of FBP as a candidate gene for maturity-onset diabetes of the young (MODY) and non-insulin-dependent diabetes (NIDDM) in both Caucasian and African-American families, using the highly informative markers D9S287 and D9S176. Although FBP is a rate-limiting enzyme in gluconeogenesis, using both parametric and nonparametric analysis there was no evidence for linkage of FBP to diabetes in these families. 30 refs., 4 figs., 2 tabs.

  20. A non-conventional fluorinated separator in high-voltage graphite/LiNi0.4Mn1.6O4 cells

    Science.gov (United States)

    Arbizzani, C.; Colò, F.; De Giorgio, F.; Guidotti, M.; Mastragostino, M.; Alloin, F.; Bolloli, M.; Molméret, Y.; Sanchez, J.-Y.

    2014-01-01

    Graphite/LiNi0.4Mn1.6O4 cells assembled with a new reinforced polyvinylidene fluoride (pVDF)-nano crystalline cellulose (NCC) separator and EC-DMC 1 M LiFAP electrolyte with additives were tested by deep charge/discharge cycles at different C-rates and by the FreedomCAR DOE protocol to simulate the dynamic functioning of the batteries in power-assist full hybrid electric vehicles (HEVs). The results of this study evidence the beneficial impact of the pVDF-NCC macroporous membrane with respect to the polypropylene monolayer Celgard®2400 separator on the high C-rate cell performance. The deep charge/discharge of the cell with pVDF-NCC at C/1 effective rate provided 101 W h kg-1 to be compared with 85 W h kg-1 of the cell with Celgard®2400 (the cell weight was considered twice the composite electrode weight of both electrodes). Also hybrid pulse power characterization tests based on the FreedomCAR protocol at 5 C and 10 C demonstrated the superior performance of the cells with pVDF-NCC with respect to that of the cells with Celgard®2400 even if both cells exceed the FreedomCAR goals of power and energy for minimum and maximum power-assist HEV.

  1. Electric control of magnetism and magnetocaloric effects in LaFe11.4Si1.6H1.5 using ferroelectric PMN-PT

    Science.gov (United States)

    Wang, Chuancong; Hu, Yong; Wang, Dunhui; Cao, Qingqi; Shao, Yanyan; Liu, Jian; Zhang, Hu; Du, Youwei

    2016-10-01

    The alloy with first-order magnetic phase transition has an advantage to exhibit large magnetoelectric effect in strain-mediated multiferroic composites, since the strain can drive its phase transition and consequently lead to a large magnetic change. In the present paper, we investigate the electric field manipulation of magnetic and magnetocaloric properties in LaFe11.4Si1.6H1.5/Pb(Mg1/3Nb2/3)O3-PbTiO3 laminate. By applying an electric field on the ferroelectric substrate, the relative change of magnetization has a peak value of  -8% around the Curie temperature, showing a large converse magnetoelectric effect. As for the magnetocaloric performance, the peak temperature of magnetic entropy change (ΔS M) has a shift of 3 K to low temperature and the maximal value of ΔS M keeps almost unchanged under an electric field of 8 kV cm-1. Moreover, the thermal and magnetic hysteresis can be reduced as well with the application of the electric field.

  2. Detection of phospholipid phase separation. A multifrequency phase fluorimetry study of 1,6-diphenyl-1,3,5-hexatriene fluorescence.

    Science.gov (United States)

    Parasassi, T; Conti, F; Glaser, M; Gratton, E

    1984-11-25

    Using multifrequency phase and modulation fluorometry and a nonlinear least-squares analysis of lifetime data, we were able to determine the complex decay of 1,6-diphenyl-1,3,5-hexatriene (DPH) in synthetic phospholipid bilayers. Our results showed a monoexponential decay of DPH in the pure isotropic solvents studied, over a wide temperature range, and a double-exponential decay of DPH in phospholipids, both above and below the transition. During the transition, and in mixed-phase phospholipids, a three-component analysis was successfully accomplished, and the pre-exponential factors of the two main components have been shown to be quantitatively representative of the gel and liquid-crystalline phases of the bilayer. The fractional intensity of the shorter lifetime component depends on the modalities of the sample preparation. The factors affecting this component are discussed. From the DPH fluorescence lifetime and from the anisotropy data in L-alpha-dimyristoyl-phosphatidylcholine/L-alpha-dipalmitoyl-phosphatidyl choline mixtures, a phase diagram was independently constructed. Conclusions about the sensitivity and the partition of the probe between gel and the liquid-crystalline phases of the bilayer are derived. Lifetime experiments on DPH in a L-alpha-dilauroyl-phosphatidylcholine/L-alpha-dipalmitoyl-phosphatidylch oline mixture suggested a general method for the determination and quantitation of the two different phases in the bilayer.

  3. Elevated neuronal excitability due to modulation of the voltage-gated sodium channel Nav1.6 by Aβ1-42

    Directory of Open Access Journals (Sweden)

    Xi eWang

    2016-03-01

    Full Text Available Aberrant increases in neuronal network excitability may contribute to the cognitive deficits in Alzheimer’s disease (AD. However, the mechanisms underlying hyperexcitability are not fully understood. Such overexcitation of neuronal networks has been detected in the brains of APP/PS1 mice. In the present study, using current-clamp recording techniques, we observed that 12 days in vitro (DIV primary cultured pyramidal neurons from P0 APP/PS1 mice exhibited a more prominent action potential burst and a lower threshold than WT littermates. Moreover, after treatment with Aβ1-42 peptide, 12 DIV primary cultured neurons showed similar changes, to a greater degree than in controls. Voltage-clamp recordings revealed that the voltage-dependent sodium current density of neurons incubated with Aβ1-42 was significantly increased, without change in the voltage-dependent sodium channel kinetic characteristics. Immunohistochemistry and western blot results showed that, after treatment with Aβ1-42, expressions of Nav and Nav1.6 subtype increased in cultured neurons or APP/PS1 brains compared to control groups. The intrinsic neuronal hyperexcitability of APP/PS1 mice might thus be due to an increased expression of voltage-dependent sodium channels induced by Aβ1-42. These results may illuminate the mechanism of aberrant neuronal networks in AD.

  4. A 1-V 10-bit 80-MS/s 1.6-mW SAR ADC in 65-nm GP CMOS

    Science.gov (United States)

    Jun, Ma; Yawei, Guo; Yue, Wu; Xu, Cheng; Xiaoyang, Zeng

    2013-08-01

    This paper presents a 10-bit 80-MS/s successive approximation register (SAR) analog-to-digital converter (ADC) suitable for integration in a system on a chip (SoC). By using the top-plate-sample switching scheme and a split capacitive array structure, the total capacitance is dramatically reduced which leads to low power and high speed. Since the split structure makes the capacitive array highly sensitive to parasitic capacitance, a three-row layout method is applied to the layout design. To overcome the charge leakage in the nanometer process, a special input stage is proposed in the comparator. As 80 MS/s sampling rate for a 10-bit SAR ADC results in around 1 GHz logic control clock, and a tunable clock generator is implemented. The prototype was fabricated in 65 nm 1P9M (one-poly-nine-metal) GP (general purpose) CMOS technology. Measurement results show a peak signal-to-noise and distortion ratio (SINAD) of 48.3 dB and 1.6 mW total power consumption with a figure of merit (FOM) of 94.8 fJ/conversion-step.

  5. The XMM deep survey in the CDF-S. IX. An X-ray outflow in a luminous obscured quasar at z~1.6

    CERN Document Server

    Vignali, C; Comastri, A; Gilli, R; Lanzuisi, G; Ranalli, P; Cappelluti, N; Mainieri, V; Georgantopoulos, I; Carrera, F J; Fritz, J; Brusa, M; Brandt, W N; Bauer, F E; Fiore, F; Tombesi, F

    2015-01-01

    In active galactic nuclei (AGN)-galaxy co-evolution models, AGN winds and outflows are often invoked to explain why super-massive black holes and galaxies stop growing efficiently at a certain phase of their lives. They are commonly referred to as the leading actors of feedback processes. Evidence of ultra-fast (v>0.05c) outflows in the innermost regions of AGN has been collected in the past decade by sensitive X-ray observations for sizable samples of AGN, mostly at low redshift. Here we present ultra-deep XMM-Newton and Chandra spectral data of an obscured (Nh~2x10^{23} cm^-2), intrinsically luminous (L2-10keV~4x10^{44} erg/s) quasar (named PID352) at z~1.6 (derived from the X-ray spectral analysis) in the Chandra Deep Field-South. The source is characterized by an iron emission and absorption line complex at observed energies of E~2-3 keV. While the emission line is interpreted as being due to neutral iron (consistent with the presence of cold absorption), the absorption feature is due to highly ionized ir...

  6. Discovery of a highly energetic pulsar associated with IGR J14003-6326 and a young uncataloged Galactic supernova remnant G310.6-1.6

    CERN Document Server

    Renaud, M; Gotthelf, E V; Rodríguez, J; Terrier, R; Mattana, F; Lebrun, F; Tomsick, J A; Manchester, R N

    2009-01-01

    We report the discovery of 31.18 ms pulsations from the INTEGRAL source IGR J14003-6326 using the Rossi X-ray Timing Explorer (RXTE). This pulsar is most likely associated with the bright Chandra X-ray source lying at the center of G310.6-1.6, a previously uncataloged Galactic composite supernova remnant with a bright central non-thermal radio and X-ray nebula, taken to be the pulsar wind nebula. PSR J1400-6325 is amongst the most energetic rotation-powered pulsars in the Galaxy, with a spin-down luminosity of Edot = 5.1E37 erg/s. In the rotating dipole model, the surface dipole magnetic field strength is B_s = 1.1E12 G and the characteristic age tau_c = P/2Pdot = 12.7 kyr. Such a high spin-down power is consistent with the hard spectral indexes of the pulsar and the nebula of 1.22+/-0.15 and 1.83+/-0.08, respectively, and a 2-10 keV flux ratio F_PWN/F_PSR ~ 8. A multi-wavelength study of this new composite supernova remnant, from radio to very-high energy gamma-rays, suggests a very young ( 6 kpc), formed by...

  7. Effect of gaseous and solid simulated jet plumes on an 040A space shuttle launch configuration at m=1.6 to 2.2

    Science.gov (United States)

    Dods, J. B., Jr.; Brownson, J. J.; Kassner, D. L.; Blackwell, K. L.; Decker, J. P.; Roberts, B. B.

    1974-01-01

    The effect of plume-induced flow separation and aspiration effects due to operation of both orbiter and the solid rocket motors on a 0.019-scale model of the launch configuration of the Space Shuttle Vehicle is determined. Longitudinal and lateral-directional stability data were obtained at Mach numbers of 1.6, 2.0, and 2.2 with and without the engines operating. The plumes exiting from the engines were simulated by a cold-gas jet supplied by an auxiliary 200-atm air supply system and solid-body plume simulators. The aerodynamic effects produced by these two simulation procedures are compared. The parameters most significantly affected by the jet plumes are pitching moment, elevon control effectiveness, axial force, and orbiter wing loads. The solid rocket motor (SRM) plumes have the largest effect on the aerodynamic characteristics. The effect of the orbiter plumes in combination with the SRM plumes is also significant. Variations in the nozzle design parameters and configuration changes can reduce the jet plume-induced aerodynamic effects.

  8. The parsec-scale distributions of intensity, linear polarization and Faraday rotation in the core and jet of Mrk501 at 8.4-1.6 GHz

    Science.gov (United States)

    Croke, S. M.; O'Sullivan, S. P.; Gabuzda, D. C.

    2010-02-01

    Previous very long baseline interferometry (VLBI) observations of the nearby (z = 0.0337) active galactic nucleus (AGN) Mrk501 have revealed a complex total-intensity structure with an approximately 90° misalignment between the jet orientations on parsec and kiloparsec scales. The jet displays a `spine' of magnetic field orthogonal to the jet surrounded by a `sheath' of magnetic field aligned with the jet. Mrk501 is also one of a handful of AGN that are regularly detected at TeV energies, indicating the presence of high-energy phenomena in the core. However, multi-epoch analyses of the VLBI total-intensity structure have yielded only very modest apparent speeds for features in the VLBI jet. We investigate the total-intensity and linear-polarization structures of the parsec- to decaparsec-scale jet of Mrk501 using VLBA observations at 8.4, 5, 2.2 and 1.6 GHz. The rotation-measure distribution displays the presence of a Faraday rotation gradient across an extended stretch of the jet, providing new evidence for a helical magnetic field associated with the jet of this AGN. The position of the radio core from the base of the jet follows the law rcore(ν) ~ ν-1.1+/-0.2, consistent with the compact inner jet region being in equipartition. Hence, we estimate a magnetic field strength of ~40 mG at a distance of 1 pc.

  9. A 1-V 10-bit 80-MS/s 1.6-mW SAR ADC in 65-nm GP CMOS

    Institute of Scientific and Technical Information of China (English)

    Ma Jun; Guo Yawei; Wu Yue; Cheng Xu; Zeng Xiaoyang

    2013-01-01

    This paper presents a 10-bit 80-MS/s successive approximation register (SAR) analog-to-digital converter (ADC) suitable for integration in a system on a chip (SoC).By using the top-plate-sample switching scheme and a split capacitive array structure,the total capacitance is dramatically reduced which leads to low power and high speed.Since the split structure makes the capacitive array highly sensitive to parasitic capacitance,a three-row layout method is applied to the layout design.To overcome the charge leakage in the nanometer process,a special input stage is proposed in the comparator.As 80 MS/s sampling rate for a 10-bit SAR ADC results in around 1 GHz logic control clock,and a tunable clock generator is implemented.The prototype was fabricated in 65 nm 1P9M (one-poly-nine-metal) GP (general purpose) CMOS technology.Measurement results show a peak signal-to-noise and distortion ratio (S1NAD) of 48.3 dB and 1.6 mW total power consumption with a figure of merit (FOM) of 94.8 fJ/conversion-step.

  10. $C_6$ coefficients and dipole polarizabilities for all atoms and many ions in rows 1-6 of the periodic table

    CERN Document Server

    Gould, Tim

    2016-01-01

    Using time-dependent density functional theory (tdDFT) with exchange kernels we calculate and test imaginary frequency-dependent dipole polarizabilities for all atoms and many ns in rows 1-6 of the periodic table. These are then integrated over frequency to produce $C_6$ coefficients. Results are presented under different models: straight tdDFT calculations using two different kernels, "benchmark" tdDFT calculations corrected by more accurate quantum chemical and experimental data, and "benchmark" tdDFT with frozen orbital anions. Parametrisations are presented for 411+ atoms and ions, allowing results to be easily used by other researchers. A curious relationship, $C_{6,XY}\\propto [\\alpha_X(0)\\alpha_Y(0)]^{0.73}$ is found between $C_6$ coefficients and static polarizabilities $\\alpha(0)$. The relationship $C_{6,XY}=2C_{6,X}C_{6,Y}/[\\alpha_X/\\alpha_YC_{6,Y}+\\alpha_Y/\\alpha_XC_{6,X}]$ is tested and found to work well ($30$\\% errors) in a small fraction of cases.

  11. Tank 241-AP-106, Grab samples, 6AP-98-1, 6AP-98-2 and 6AP-98-3 Analytical results for the final report

    Energy Technology Data Exchange (ETDEWEB)

    FULLER, R.K.

    1999-02-23

    This document is the final report for tank 241-AP-106 grab samples. Three grab samples 6AP-98-1, 6AP-98-2 and 6AP-98-3 were taken from riser 1 of tank 241-AP-106 on May 28, 1998 and received by the 222-S Laboratory on May 28, 1998. Analyses were performed in accordance with the ''Compatability Grab Sampling and Analysis Plan'' (TSAP) (Sasaki, 1998) and the ''Data Quality Objectives for Tank Farms Waste Compatability Program (DQO). The analytical results are presented in the data summary report. No notification limits were exceeded. The request for sample analysis received for AP-106 indicated that the samples were polychlorinated biphenyl (PCB) suspects. The results of this analysis indicated that no PCBs were present at the Toxic Substance Control Act (TSCA) regulated limit of 50 ppm. The results and raw data for the PCB analysis are included in this document.

  12. A direct detection 1.6μm DIAL with three wavelengths for high accuracy measurements of vertical CO2 concentration and temperature profiles

    Science.gov (United States)

    Shibata, Yasukuni; Nagasawa, Chikao; Abo, Makoto

    2013-10-01

    The accurate vertical CO2 profiles in the troposphere are highly desirable in the inverse techniques to improve quantification and understanding of the global budget of CO2 and also global climate changes. Moreover, wind information is an important parameter for transport simulations and inverse estimation of surface CO2 flux. A differential absorption lidar (DIAL) is an attractive method for obtaining vertical CO2 profiles and we have developed an 1.6μm DIAL system to perform simultaneous measurements of CO2 concentration, atmospheric temperature profile and wind profile. The absorption cross sections of gas and air density depends on atmospheric temperature and pressure. Then precise temperature and pressure profiles are necessary for accurate CO2 mixing ratio measurement by DIAL. Laser beams of three wavelengths around a CO2 absorption line are transmitted alternately to the atmosphere for simultaneous measurements of CO2 concentration and temperature. The receiving optics include the near-infrared photomultiplier tube and a fiber Bragg grating (FBG) filter to detect a Doppler shift.

  13. Loss of fructose-1,6-bisphosphatase induces glycolysis and promotes apoptosis resistance of cancer stem-like cells: an important role in hexavalent chromium-induced carcinogenesis.

    Science.gov (United States)

    Dai, Jin; Ji, Yanli; Wang, Wei; Kim, Donghern; Fai, Leonard Yenwong; Wang, Lei; Luo, Jia; Zhang, Zhuo

    2017-09-15

    Hexavalent chromium (Cr(VI)) compounds are confirmed human carcinogens for lung cancer. Our previous studies has demonstrated that chronic exposure of human bronchial epithelial BEAS-2B cells to low dose of Cr(VI) causes malignant cell transformation. The acquisition of cancer stem cell-like properties is involved in the initiation of cancers. The present study has observed that a small population of cancer stem-like cells (BEAS-2B-Cr-CSC) exists in the Cr(VI)-transformed cells (BEAS-2B-Cr). Those BEAS-2B-Cr-CSC exhibit extremely reduced capability of generating reactive oxygen species (ROS) and apoptosis resistance. BEAS-2B-Cr-CSC are metabolic inactive as evidenced by reductions in oxygen consumption, glucose uptake, ATP production, and lactate production. Most importantly, BEAS-2B-Cr-CSC are more tumorigenic with high levels of cell self-renewal genes, Notch1 and p21. Further study has found that fructose-1,6-bisphosphatase (FBP1), an rate-limiting enzyme driving glyconeogenesis, was lost in BEAS-2B-Cr-CSC. Forced expression of FBP1 in BEAS-2B-Cr-CSC restored ROS generation, resulting in increased apoptosis, leading to inhibition of tumorigenesis. In summary, the present study suggests that loss of FBP1 is a critical event in tumorigenesis of Cr(VI)-transformed cells. Copyright © 2017 Elsevier Inc. All rights reserved.

  14. A Hubble Space Telescope Lensing Survey of X-ray Luminous Galaxy Clusters A Multiply-imaged Extremely Red Galaxy at z=1.6

    CERN Document Server

    Smith, G P; Kneib, J P; Davis, C J; Takamiya, M; Ebeling, H; Czoske, O; Smith, Graham P.; Smail, Ian

    2002-01-01

    We present near-infrared spectroscopy and Hubble Space Telescope (HST) imaging of EROJ003707+0909.5, the brightest of three gravitationally-lensed images of an Extremely Red Object (ERO) at z=1.6, in the field of the massive cluster A68 (z=0.255). We exploit the superlative resolution of our HST data and the enhanced spatial resolution and sensitivity afforded by the lens amplification to reconstruct the source-plane properties of this ERO. Our morphological and photometric analysis reveals that EROJ003707 is an L* early-type disk-galaxy and we estimate that ~10 per cent of EROs with (R-K)>=5.3 and K<=21 may have similar properties. The unique association of passive EROs with elliptical galaxies therefore appears to be too simplistic. We speculate on the evolution of EROJ003707: if gas continues to cool onto this galaxy in the manner predicted by hierarchical galaxy formation models, then by the present day, EROJ003707 could evolve into a very luminous spiral galaxy.

  15. Effects of n-3 fatty acid, fructose-1,6-diphosphate and glutamine on mucosal cell proliferation and apoptosis of small bowel graft after transplantation in rats

    Institute of Scientific and Technical Information of China (English)

    Xiao-Ting Wu; Jie-Shou Li; Xiao-Fei Zhao; Ning Li; Yu-Kui Ma; Wen Zhuang; Yong Zhou; Gang Yang

    2003-01-01

    AIM: To evaluate the effects of n-3 fatty acids (n-3FA),fructose-1,6-diphosphate (FDP) and glutamine (GLN) on mucosal cell proliferation and apoptosis of small bowel graft. METHODS: One hundred and ninety-six inbred strain Wistar rats were grouped as donors and recipients, and underwent heterotopic small bowel transplantation (SBT). n-3FA, FDP and GLN were administered via gastric tube as well as venous infusion for 10 days before and after surgery, respectively.The proliferation and apoptosis of mucosal cells were analyzed with flow cytometry and in situ cell death detection kits. RESULTS: Apparent apoptosis and minor proliferation of mucosal cells of small bowel graft after transplantation were observed. A higher mucosal cell proliferative index and lower apoptotic index were found in all small bowel grafts after supplying with n-3FA, FDP and GLN. CONCLUSION: Nutritional support with n-3FA, FDP and GLN promotes mucosal cell proliferation significantly, and prevents mucosal cell from undergoing apoptosis with different degrees. These regulatory effects on the apoptosis alter the structure and absorption function of transplanted small bowel favorably.

  16. Fractal and probability analysis of creep crack growth behavior in 2.25Cr–1.6W steel incorporating residual stresses

    Energy Technology Data Exchange (ETDEWEB)

    Xu, Mengjia; Xu, Jijin, E-mail: xujijin_1979@sjtu.edu.cn; Lu, Hao; Chen, Jieshi; Chen, Junmei; Wei, Xiao

    2015-12-30

    Graphical abstract: - Highlights: • Statistical and fractal analysis is applied to study the creep fracture surface. • The tensile residual stresses promote the initiation of creep crack. • The fractal dimension of a mixed mode fracture surface shows a wavy variation. • The fractal dimension increases with increasing intergranular fracture percentage. • Height coordinates of intergranular fracture surface fit Gaussian distribution. - Abstract: In order to clarify creep crack growth behavior in 2.25Cr–1.6W steel incorporating residual stresses, creep crack tests were carried out on the tension creep specimens, in which the residual stresses were generated by local remelting and cooling. Residual stresses in the specimens were measured using Synchrotron X-ray diffraction techniques. The fracture surface of the creep specimen was analyzed using statistical methods and fractal analysis. The relation between fractal dimension of the fracture surface and fracture mode of the creep specimen was discussed. Due to different fracture mechanisms, the probability density functions of the height coordinates vary with the intergranular crack percentage. Good fitting was found between Gaussian distribution and the probability function of height coordinates of the high percentage intergranular crack surface.

  17. Molecules in G1.6-0.25 - 'Hot' Chemistry in the Absence of Star Formation at the Periphery of the Galactic Center Region

    CERN Document Server

    Menten, Karl M; Leurini, Silvia; Schilke, Peter

    2008-01-01

    We present molecular line mapping of the Giant Molecular Cloud G1.6-0.025, which is located at the high longitude end of the Central Molecular Zone of our Galaxy. We assess the degree of star formation activity in that region using several tracers and find very little. We made a large scale, medium (2') resolution map in the J = 2-1 transition of SiO for which we find clumpy emission over a ~0.8 x 0.3 degree-sized region stretching along the Galactic plane. Toward selected positions we also took spectra in the easy to excite J_k=2_k-1_k quartet of CH3OH and the CS 2-1 line. Throughout the cloud these \\meth lines are, remarkably, several times stronger than, both, the CS and the SiO lines. The large widths of all the observed lines, similar to values generally found in the Galactic center, indicate a high degree of turbulence. Several high LSR velocity clumps that have 0-80 km/s higher velocities than the bulk of the molecular cloud appear at the same projected position as "normal" velocity material; this may ...

  18. The FMOS-COSMOS survey of star-forming galaxies at z ~ 1.6 I. H\\alpha -based star formation rates and dust extinction

    CERN Document Server

    Kashino, D; Rodighiero, G; Renzini, A; Arimoto, N; Daddi, E; Lilly, S J; Sanders, D B; Kartaltepe, J; Zahid, H J; Nagao, T; Sugiyama, N; Capak, P; Carollo, C M; Chu, J; Hasinger, G; Ilbert, O; Kajisawa, M; Kewley, L J; Koekemoer, A M; Kovač, K; Fèvre, O Le; Masters, D; McCracken, H J; Onodera, M; Scoville, N; Strazzullo, V; Symeonidis, M; Taniguchi, Y

    2013-01-01

    We present first results from a near-infrared spectroscopic survey of the COSMOS field, using the Fiber Multi-Object Spectrograph (FMOS) on the Subaru telescope, designed to characterize the star-forming galaxy population at 1.41.6-1.8\\mu m with $f_H\\alpha\\ \\gtrsim 4 \\times 10^{-17} ergs cm^{-2} s^{-1}$. Here, we specifically focus on 271 sBzK-selected galaxies that yield a H\\alpha\\ detection thus providing a redshift and emission-line luminosity to establish the relation between star formation rate (SFR) and stellar mass. With further J-band spectroscopy for 89 of these, the level of dust extinction is assessed by measuring the Balmer decrement using co-added spectra. We find that the extinction (0.6\\lesssim A_H\\alpha\\ \\lesssim 2.5) rises with stellar mass and is elevated at high masses compared to low-redshift galaxies. Using this subset of the spectroscopic sample, we further find that the differential extinction b...

  19. Theoretical studies on the electronic structures and photoelectron spectra of tri-rhenium oxide clusters: Re3On- and Re3On (n = 1-6)

    Science.gov (United States)

    Zhou, Qi; Gong, Wei-Chao; Xie, Lu; Zheng, Cun-Gong; Zhang, Wei; Wang, Bin; Zhang, Yong-Fan; Huang, Xin

    2014-01-01

    Density functional theory (DFT) calculations are performed to study the structural and electronic properties of tri-rhenium oxide clusters Re3On-/0 (n = 1-6). Generalized Koopmans' theorem is applied to predict the vertical detachment energies (VDEs) and simulate the photoelectron spectra (PES). Theoretical calculations at the B3LYP level are carried out to search for the global minima for both the anions and the neutrals. For the anions, the first two O atoms prefer the same corner position of a Re3 triangle. Whereas, Re3O3- possesses a C2v symmetry with one bridging and two terminal O atoms. The next three O atoms (n = 4-6) are adding sequentially on the basis of Re3O3- motif, i.e., adding one terminal O atom for Re3O4-, one terminal and one bridging O atoms for Re3O5-, and one terminal and two bridging O atoms for Re3O6-, respectively. Their corresponding neutral species are similar to the anions in geometry except Re3O4 and Re3O5. Molecular orbital analyses are employed to investigate the chemical bonding and structural evolution in these tri-rhenium oxide clusters.

  20. Excitation of the Delta(1232) isobar in deuteron charge exchange on hydrogen at 1.6, 1.8, and 2.3 GeV

    CERN Document Server

    Mchedlishvili, D; Carbonell, J; Chiladze, D; Dymov, S; Dzyuba, A; Engels, R; Gebel, R; Glagolev, V; Grigoryev, K; Goslawski, P; Hartmann, M; Imambekov, O; Kacharava, A; Kamerdzhiev, V; Keshelashvili, I; Khoukaz, A; Komarov, V; Kulessa, P; Kulikov, A; Lehrach, A; Lomidze, N; Lorentz, B; Macharashvili, G; Maier, R; Merzliakov, S; Mielke, M; Mikirtychyants, M; Mikirtychyants, S; Nioradze, M; Ohm, H; Papenbrock, M; Prasuhn, D; Rathmann, F; Serdyuk, V; Seyfarth, H; Stein, H J; Steffens, E; Stockhorst, H; Ströher, H; Tabidze, M; Trusov, S; Uzikov, Yu; Valdau, Yu; Wilkin, C

    2013-01-01

    The charge-exchange break-up of polarised deuterons pol{d}p -> {pp}n, where the final {pp} diproton system has a very low excitation energy and hence is mainly in the 1S0 state, is a powerful tool to probe the spin-flip terms in the proton-neutron charge-exchange scattering. Recent measurements with the ANKE spectrometer at the COSY storage ring at 1.6, 1.8, and 2.27 GeV have extended these studies into the pion-production regime in order to investigate the mechanism for the excitation of the Delta(1232) isobar in the pol{d}p -> {pp}X reaction. Values of the differential cross section and two deuteron tensor analysing powers, A_{xx} and A_{yy}, have been extracted in terms of the momentum transfer to the diproton or the invariant mass Mx of the unobserved system X. The unpolarised cross section in the high Mx region is well described in a model that includes only direct excitation of the Delta isobar through undistorted one pion exchange. However, the cross section is grossly underestimated for low Mx, even w...