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Sample records for vitro del pinus

  1. In vitro regeneration of Pinus brutia Ten. var. eldarica (Medw ...

    African Journals Online (AJOL)

    This paper describes two in vitro regeneration systems through direct and indirect organogenesis in Pinus brutia using fascicles aseptic cultures as explants. Mechanical scarification and gibberellic acid (GA3) were evaluated on in vitro seed germination. Scarification was the treatment that allowed for in vitro seed ...

  2. DETERMINACIÓN DE MEDIOS DE CULTIVO Y PH PARA LA MASIFICACIÓN in vitro DE CEPAS DE Suillus luteus Aubl. ASOCIADAS A Pinus radiata D. Don Y Scleroderma citrinum Pers. ASOCIADAS A Eucalyptus globulus Labill. DE LA REGIÓN DEL BIOBÍO, CHILE

    National Research Council Canada - National Science Library

    Marta González; Iván Quiroz; Ruy Travieso; Patricio Chung; Edison García

    2015-01-01

    ... óptimo para crecer, asociarse y reproducirse. El objetivo de este estudio fue determinar la dependencia del pH y el medio de cultivo en el crecimiento in vitro de distintas cepas de Suillus luteus y Scleroderma citrinum asociadas a Pinus radiata...

  3. Evaluacion de las condiciones de extraccion por hidrodestilacion -cohobacion del aceite esencial del follaje de Pinus caribaea Morelet var. caribaea

    National Research Council Canada - National Science Library

    Fernandez-Sanchez, Felix; Marin-Moran, Jorge Eric; Teixeira-Pinto, Zeneida; de Carvalho-Queiro, Margareth Maria; Escalona-Arranz, Julio Cesar

    2013-01-01

    ...) del aceite esencial del follaje de Pinus caribaea Morelet var. caribaea (droga seca). Los factores estudiados en el proceso de extraccion fueron el tamano de particula (1,25; 0,6; 0,25 y 0,18 mm...

  4. Abundancia y distribucion del genero Pinus en Capulalpam de Mendez, Sierra Juarez, Oaxaca

    National Research Council Canada - National Science Library

    Rios-Altamirano, Arianne; Alfonso-Corrado, Cecilia; Aguirre-Hidalgo, Victor; Angeles-Perez, Gregorio; Mendoza-Diaz, Magdalena M; Rodriguez-Rivera, Viviana; Roldan-Felix, Eusebio; Clark-Tapia, Ricardo

    2016-01-01

    Se analizan los factores ambientales que determinan la abundancia y distribucion de las diferentes especies comerciales y aprovechadas del genero Pinus en el municipio de Capulalpam de Mendez, Oaxaca...

  5. Basic aspects of in vitro propagation of Pinus genus by organogenesis

    Directory of Open Access Journals (Sweden)

    Maité Chávez

    2012-07-01

    Full Text Available Plant regeneration in vitro of Pinus genus by somatic embryogenesis has been long studied. However, the in vitro establishment of plants from apical shoots and in vitro multiplication of these shoots have been an alternative less studied. Therefore the production of plants, regenerated by organogenesis, able to survive in the acclimatization phase, has been limited. This work aims to present a review of the literature about the in vitro propagation by organogenesis of the genus Pinus, including an analysis of the main factors that could, somehow, affect the success of plant regeneration by this method with emphasis on in vitro and ex vitro root formation.   Key words: apical shoots, ex vitro rooting, in vitro rooting, plants regeneration, pine.

  6. Water supply offered by alternative substrates used in in vitro Pinus taeda L. germination/Subsidio hidrico fornecido por substratos alternativos usados na germinacao in vitro de Pinus taeda L

    National Research Council Canada - National Science Library

    Golle, Diego Pascoal; Reiniger, Lia Rejane Silveira; Muniz, Marlove Fatima Briao; Curti, Aline Ritter; da Rosa, Felippe Correa

    2009-01-01

    The aim of this study was to assess the water supply provided by different alternative substrates as well as to analyze its efficiency in the sanity and in the in vitro germination of Pinus taeda L. seeds...

  7. DETERMINACIÓN DE MEDIOS DE CULTIVO Y PH PARA LA MASIFICACIÓN in vitro DE CEPAS DE Suillus luteus Aubl. ASOCIADAS A Pinus radiata D. Don Y Scleroderma citrinum Pers. ASOCIADAS A Eucalyptus globulus Labill. DE LA REGIÓN DEL BIOBÍO, CHILE

    Directory of Open Access Journals (Sweden)

    Marta González

    2015-02-01

    Full Text Available El uso de agentes biológicos en la producción en vivero ha aflorado como un elemento que permite no sólo mejorar la calidad morfológica y fisiológica de las plantas, sino que también posibilitar un mayor éxito en el ámbito silvicultural de una plantación. Estos agentes biológicos requieren de un ambiente óptimo para crecer, asociarse y reproducirse. El objetivo de este estudio fue determinar la dependencia del pH y el medio de cultivo en el crecimiento in vitro de distintas cepas de Suillus luteus y Scleroderma citrinum asociadas a Pinus radiata y Eucalyptus globulus, respectivamente. El estudio se realizó en condiciones controladas de temperatura y humedad, disponiendo los inóculos en placas Petri con diferentes medios cultivo, evaluando parámetros de crecimiento y biomasa a los 38 días para S. luteus y 105 días para S. citrinum. Los resultados indican que tanto el medio de cultivo, el pH del medio, así como las cepas de cada especie estudiada, son determinantes en las respuestas de crecimiento de los hongos ectomicorrícicos in vitro evaluados. Las cepas de S. luteus se desarrollaron adecuadamente en un medio de cultivo con abundancia de nutrientes (BAF, MMN como en baja presencia de ellos (EMA, y con pH más bien ácido (4,8 y 5,8. Por otro lado, las cepas de S. citrinum presentaron, para los mismos ambientes, un desarrollo inferior y lento, no obstante, la cepa Sc8 se reprodujo de forma óptima y rápida bajo un medio de cultivo BAF y con un pH moderadamente ácido de 5,8.

  8. Inhibición de la replicación del virus de inmunodeficiencia humana por extractos de taninos de Pinus caribaea Morelet

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    Ignacio Juan Ruibal Brunet

    2003-08-01

    Full Text Available Diferentes concentraciones de 6 extractos de corteza de Pinus caribaea Morelet var. caribaea se enfrentaron a 2 dosis de virus en un ensayo in vitro, sobre células MT4; la actividad antiviral se midió por ensayo inmunoenzimático de captura de proteína 24 del virus. Todas las fracciones mostraron actividad citotóxica moderada y solo una fue altamente tóxica. La fracción 02 mostró un alto porcentaje de inhibición de la replicación viral, en relación con la dosis viral y la concentración del producto, con un índice de selectividad de 100, pero son necesarios estudios adicionales sobre la identificación de la estructura química para definir el mecanismo de acción del producto.Six different fractions from the bark of Pinus caribae Moralet var. caribae were faced in five different concentrations against two viral doses (MOI 0,1 y 0,01 in a vitro assay on MT4 cell lines; the antiviral activity was measured by p24 Ag capture ELISA assay (DAVIH Agp24. All the fractions showed a mild cytotoxicity activity and only one fraction showed the highest cytotoxicity activity. The fraction 02 had the highest percentage of viral replication inhibition, correlated with the viral dose and the product concentration, having a selectivity rate of 100; however, more research about the chemical structure of active compounds, and possible mechanisms of action are needed.

  9. Characterization and biological activity of Taishan Pinus massoniana pollen polysaccharide in vitro.

    Directory of Open Access Journals (Sweden)

    Shifa Yang

    Full Text Available Taishan Pinus massoniana pollen polysaccharide (TPPPS improves cellular and humoral immune responses of animals and is a novel potential immunomodulator. However, the components of TPPPS have not been recognized. To investigate the composition of TPPPS, crude polysaccharide was obtained from Taishan P. massoniana pollen through water extraction and ethanol precipitation. Three homogeneous polysaccharide fractions (TPPPS1, TPPPS2, and TPPPS3 were purified from TPPPS by DEAE-cellulose column chromatography. The average molecular weights of the three polysaccharides were 56, 25, and 128 kDa, respectively. Results of high-performance liquid chromatography (HPLC showed that TPPPS comprised mannose, ribose, xylose, glucuronic acid, galacturonic acid, glucose, galactose, and arabinose. The biological activity assays showed that TPPPS2 and TPPPS3 significantly promoted spleen lymphocyte proliferation, and that TPPPS3 showed better effect than TPPPS2. TPPPS3 enhanced the secretion of cytokine IL-2 and TNF, whereas TPPPS2 mainly elevated IL-2 secretion. By contrast, TPPPS1 exhibited other effects, and it induced the highest amount of NO production, thereby indicating that TPPPS1 had the best antioxidant activity. TPPPS3 at 50 μg/mL significantly inhibited the proliferation of subgroup B Avian Leukosis virus (ALV-B through virus adsorption interference in vitro. Results indicated that TPPPS comprised three main components, among which, TPPPS1 mainly showed antioxidant effects, whereas TPPPS2 and TPPPS3 played key roles in immunomodulation, especially TPPPS3. Further studies on the use of a reasonable proportion of TPPPS1-3 may facilitate the development of an effective immunomodulator.

  10. In vitro co-cultures of Pinus pinaster with Bursaphelenchus xylophilus: a biotechnological approach to study pine wilt disease.

    Science.gov (United States)

    Faria, Jorge M S; Sena, Inês; Vieira da Silva, Inês; Ribeiro, Bruno; Barbosa, Pedro; Ascensão, Lia; Bennett, Richard N; Mota, Manuel; Figueiredo, A Cristina

    2015-06-01

    Co-cultures of Pinus pinaster with Bursaphelenchus xylophilus were established as a biotechnological tool to evaluate the effect of nematotoxics addition in a host/parasite culture system. The pinewood nematode (PWN), Bursaphelenchus xylophilus, the causal agent of pine wilt disease (PWD), was detected for the first time in Europe in 1999 spreading throughout the pine forests in Portugal and recently in Spain. Plant in vitro cultures may be a useful experimental system to investigate the plant/nematode relationships in loco, thus avoiding the difficulties of field assays. In this study, Pinus pinaster in vitro cultures were established and compared to in vivo 1 year-old plantlets by analyzing shoot structure and volatiles production. In vitro co-cultures were established with the PWN and the effect of the phytoparasite on in vitro shoot structure, water content and volatiles production was evaluated. In vitro shoots showed similar structure and volatiles production to in vivo maritime pine plantlets. The first macroscopic symptoms of PWD were observed about 4 weeks after in vitro co-culture establishment. Nematode population in the culture medium increased and PWNs were detected in gaps of the callus tissue and in cavities developed from the degradation of cambial cells. In terms of volatiles main components, plantlets, P. pinaster cultures, and P. pinaster with B. xylophilus co-cultures were all β- and α-pinene rich. Co-cultures may be an easy-to-handle biotechnological approach to study this pathology, envisioning the understanding of and finding ways to restrain this highly devastating nematode.

  11. Contenido del aceite esencial en el follaje de Pinus Caribaea morelet en función de la edad del árbol. II

    Directory of Open Access Journals (Sweden)

    Rolando Quert Álvarez

    2000-08-01

    Full Text Available Se presentan los resultados obtenidos en la cuantificación del contenido de aceite esencial de la especie Pinus caribaea Morelet endémica de Cuba, en función de la edad del árbol. El estudio se realizó con árboles existentes en áreas de la Estación Experimental Forestal de Viñales, provincia de Pinar del Río, con edades de entre 8-30 a. El tamaño de muestra fue de 3 árboles y el tiempo de destilación para la extracción del aceite esencial de 3 h. Los resultados obtenidos demostraron que el contenido de aceite esencial varía significativamente con la edad del árbol en forma ascendente, con el valor más bajo (0,12 % en peso a los 8 a y el más alto (0,27 % en peso a los 30 a.The results obtained in the quantitation of the content of essential oil of the Pinus caribaea Morelet, an endemic species from Cuba, according to the age of the tree, are presented. The study was conducted with trees existing in the areas of the Experimental Forestal Station in Viñales, province of Pinar del Río, at ages 8-30. The size of the sample was of 3 trees and the distillation time for the extraction of essential oil was of 3 hours. The results obtained showed that the content of esential oil increases significantly with age. The lowest value (0.12% in weight was registered at 8 and the highest (0,27% in weight at 30.

  12. HISTOPATOLOGÍA DEL PINO DE LAS ALTURAS (Pinus hartwegii Lindl. INOCULADO CON TRES HONGOS OPHIOSTOMATOIDES

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    Omar Alejandro Pérez-Vera

    2011-01-01

    Full Text Available El objetivo de este estudio fue identificar las alteraciones histológicas en el pino de las alturas (Pinus hartwegii causadas por tres especies de hongos ophiostomatoides, por medio de microscopia de luz. En Pinus hartwegii hubo acumulación de resina en la zona de inoculación a los diez días, y el follaje se tornó de amarillo a café rojizo a los 60 días. En las inoculaciones con Leptographium guttulatum y Ophiostoma olivaceapinii se observó que inducen la metabolización de polifenoles, depositándose en las paredes de las células de la corteza, el floema, cambium vascular y en la médula se necrosaron. O. ips causó necrosis más severa en corteza, floema, cambium vascular y médula. En xilema, las hifas de los tres hongos penetran en las traqueidas y avanzan longitudinalmente por las puntuaciones aeroladas y se distribuyen radialmente por células parenquimatosas y radios.

  13. Contenido del aceite esencial en el follaje de Pinus caribaea Morelet según la época del Año. I

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    Rolando Quert Álvarez

    1998-04-01

    Full Text Available Se realizó un estudio con el objetivo de determinar el contenido de aceite esencial en el follaje verde de la especie Pinus caribaea Morelet y cómo varía éste según los diferentes meses del año. Se determinaron el tiempo de destilación del aceite esencial a escala de laboratorio, el tamaño de muestra en nuestras condiciones de trabajo y el contenido de aceite esencial durante 2 años, mediante el análisis del contenido de aceite esencial de enero a diciembre. El tiempo de destilación obtenido fue de 3 h y el tamaño de muestra de 3 árboles. Los más altos rendimientos de aceite esencial se obtuvieron en los meses de marzo y abril y los más bajos entre noviembre y diciembre.A study was conducted aimed at determining the content of essential oil in the green foliage of the Pinus caribea Morelet species and at knowing how it varies according to the month of the year. The time of distillation of the essential oil at laboratory scale, the size of the sample under our working conditions, and the content of essential oil during two years were determined by analyzing the content of essential oil from January to December. The distillation time obtained was of 3 hours and the size of the sample was of 3 trees. The highest yields of essential oil were obtained in March and April, where as the lowest ones were attained between November and December.

  14. CONCENTRACIÓN DE CARBONO EN Pinus cembroides Zucc: FUENTE POTENCIAL DE MITIGACIÓN DEL CALENTAMIENTO GLOBAL

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    Marín Pompa-García

    2014-01-01

    Full Text Available Los bosques de Pinus cembroides Zucc, usualmente, han sido segregados del aprovechamiento forestal comercial dadas sus desventajas maderables. Sin embargo, esta especie desempeña un papel importante en la biodiversidad de México. El objetivo del presente estudio fue deter - minar la concentración de carbono (C en los principales compartimentos de P. cembroides : raíz, corteza, fuste, ramas, yemas y hojas. La concentración de C total expresado como porcentaje de la biomasa fue determinado con el Solids TOC Analyzer. Los resultados se analizaron en un diseño completamente al azar y mediante comparación de medias de Tukey. El análisis indicó que la concen - tración de C var ía entre los componentes ( P < 0.05; la yema tuvo el valor más alto (57.1 %, mientras que el fuste y las ramas registraron los valores más bajos (47.7 y 47.8 %, respectivamente. Los resul - tados contribuyen a mejorar las estimaciones de C y proveen información importante para definir si los bosques de P. cembroides pueden considerarse como una posible fuente con potencial de mitigación ante el cambio climático y así utilizarlos en un programa de secuestro de C.

  15. Efectos de quemas prescritas sobre las propiedades del suelo en bosques de Pinus tropicalis Morelet, en Cuba

    Directory of Open Access Journals (Sweden)

    L. W. Martínez Becerra

    2004-01-01

    Full Text Available El experimento se desarrolló en la Empresa Forestal Integral “La Palma”, provincia Pinar del Río, Cuba. Su objetivo fue evaluar el efecto de las quemas prescritas sobre las propiedades del suelo en bosques naturales de Pinus tropicalis Morelet. Se ubicaron cuatro parcelas de 1,000 m2. Una fue el testigo y en las restantes se aplicó quema. Para la obtención de los datos fueron colocados aleatoriamente cinco puntos de muestreo en cada parcela. Una semana antes, una después y al año de aplicada las quemas prescritas, se tomaron muestras de suelo a profundidades de 0 a 10 cm y de 10 a 20 cm. Los datos se analizaron a través de pruebas de comparación de medias (ANOVA. Los resultados muestran un ligero aumento (no significativo para el pH, el P2O5 (fósforo cambiable, el Mg y el K (potasio asimilable, al año de efectuadas las quemas, por otra parte, disminuyeron no significativamente el K2O (cambiable, la materia orgánica (MO y el Ca. Al año de efectuada la quema aumenta significativamente el contenido de Na, disminuyendo significativamente la acidez hidrolítica y la capacidad de cambio catiónico a las dos profundidades antes y después de la quema. La relación entre los nutrientes del suelo se encuentra dentro de los rangos típicos de estos suelos pobres.

  16. EFECTO DEL RALEO SOBRE EL CRECIMIENTO Y LA DENSIDAD DE LA MADERA DE Pinus taeda IMPLANTADO EN MISIONES, ARGENTINA

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    Rodolfo Martiarena

    2014-09-01

    Full Text Available http://dx.doi.org/10.5902/1980509815746El mayor incremento en crecimiento de la plantación puede causar variación en las propiedades físicas de la madera, pudiendo disminuir la calidad de la misma para la industria. El objetivo del presente trabajo es evaluar el efecto de los tratamientos de raleo sobre el crecimiento y densidad de la madera de Pinus taeda implantado en Misiones, Argentina (25º 59’S - 54º24’O. La plantación se estableció en 1985 con densidad inicial de 1644 pl.ha-1y se manejó con tres intensidades de raleo (0, 33 y 66 % del área basal del testigo sin ralear, bajo un diseño de bloques completos al azar (3 bloques. A los 20 años se efectuó tala rasa, cuyas densidades eran 711 (0 %, 364 (33 % y 122 (66 % plantas por hectárea, momento en el cual se seleccionaron 45 árboles para determinar la densidad básica de la madera. La misma se determinó a través de la realización de 2.700 probetas. El crecimiento de la plantación fue afectado por la intensidad de raleo, registrando diámetros promedio de 28,7, 34,8 y 45,9 cm, respectivamente, en los tratamientos 0, 33 y 66 % de raleo. El volumen de la plantación, al momento del apeo, fue superior en el tratamiento sin raleo, no obstante el tratamiento 66 % de raleo generó mayor cantidad de madera de grandes dimensiones. La densidad básica como promedio simple y ponderado por volumen no presentó diferencias significativas entre tratamientos. Los valores de densidad ponderada fueron de 0,406, 0,418 y 0,420 g.cm-3 para los tratamientos 0, 33 y 66 % de raleo, respectivamente, mientras que el análisis de correlación entre diámetro y densidad no fue significativo. El estudio mostró que la intensidad de raleo puede ser manejada para maximizar la producción, manteniendo idéntica la calidad de la madera para la industria.

  17. EFECTO DEL RALEO SOBRE EL CRECIMIENTO Y LA DENSIDAD DE LA MADERA DE Pinus taeda IMPLANTADO EN MISIONES, ARGENTINA

    Directory of Open Access Journals (Sweden)

    Rodolfo Martiarena

    2014-01-01

    Full Text Available El mayor incremento en crecimiento de la plantación puede causar variación en las propiedades físicas de la madera, pudiendo disminuir la calidad de la misma para la industria. El objetivo del presente trabajo es evaluar el efecto de los tratamientos de raleo sobre el crecimiento y densidad de la madera de Pinus taeda implantado en Misiones, Argentina (25º 59’S - 54º24’O. La plantación se estableció en 1985 con densidad inicial de 1644 pl.ha -1 y se manejó con tres intensidades de raleo (0, 33 y 66 % del área basal del testigo sin ralear, bajo un diseño de bloques completos al azar (3 bloques. A los 20 años se efectuó tala rasa, cuyas densidades eran 711 (0 %, 364 (33 % y 122 (66 % plantas por hectárea, momento en el cual se seleccionaron 45 árboles para determinar la densidad básica de la madera. La misma se determinó a través de la realización de 2.700 probetas. El crecimiento de la plantación fue afectado por la intensidad de raleo, registrando diámetros promedio de 28,7, 34,8 y 45,9 cm, respectivamente, en los tratamientos 0, 33 y 66 % de raleo. El volumen de la plantación, al momento del apeo, fue superior en el tratamiento sin raleo, no obstante el tratamiento 66 % de raleo generó mayor cantidad de madera de grandes dimensiones. La densidad básica como promedio simple y ponderado por volumen no presentó diferencias significativas entre tratamientos. Los valores de densidad ponderada fueron de 0,406, 0,418 y 0,420 g.cm -3 para los tratamientos 0, 33 y 66 % de raleo, respectivamente, mientras que el análisis de correlación entre diámetro y densidad no fue significativo. El estudio mostró que la intensidad de raleo puede ser manejada para maximizar la producción, manteniendo idéntica la calidad de la madera para la industria.

  18. Estudio del potencial energético de biomasa Pinus caribaea Morelet var. Caribaea (Pc Y Pinus tropicalis Morelet (Pt; Eucaliptus saligna Smith (Es, Eucalyptus citriodora Hook (Ec y Eucalytus...

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    F. Márquez Montesino

    2001-01-01

    Full Text Available Los residuos forestales y de plantas agrícolas leñosas constituyen recursos potenciales con interesantes posibilidades de aprovechamiento tecnológico, entre los que cabe destacar el empleo de procesos termoquímicos, combustión, gasificación y pirólisis. La valoración energética de un determinado material debe considerar como elemento fundamental su poder calorífico. En el presente trabajo se presentan los resultados obtenidos sobre las posibilidades de aprovechamiento energético de diversos residuos forestales, abundantes en la provincia de Pinar del Río tales como Pinus caribaea Morelet var. Caribaea (Pc, y Pinus tropicalis Morelet (Pt; madera de eucaliptos, Eucaliptus saligna Smith (Es, Eucalyptus citriodora Hook (Ec y Eucalytus pellita F. Muell (Ep; carbonizados de aserrín preparados a escala de laboratorio, a una temperatura de 700 °C y 2 horas de carbonización y carbones vegetales preparados en horno de parva a partir de Pinus caribaea Morelet var. caribaea, (Costanera, (PcC y otras maderas de especies naturales tales como Dicrostachys cinerea, Wight & Arm., (Marabú, (Dc; Conocarpus erecta L., (Yana, (Ce; Quercus oleoide C. y S. var. Sagreana C.H. Mull, (Encina, (Qo y Guasuma tomentosa HBK, (Guásima (Gt. Se ha establecido una ecuación que correlaciona el poder calorífico de diversos materiales biomásicos con sus contenidos en materia volátil y carbono fijo, a través de un análisis por regresión lineal múltiple. Esta ecuación ha resultado ser válida para residuos agroforestales húmedos, secos o carbonizados con distinto grado de carbonización, obteniéndose valores de poderes caloríficos del orden de 4.500 kcal⋅kg-1 (20 kJ⋅g-1 para las biomasas estudiadas y de hasta 8000 kcal⋅kg-1 (33,5 kJ⋅g-1 para los carbones vegetales obtenidos en el laboratorio. Por tanto, estos materiales presentan atractivas posibilidades de aprovechamiento energético como materia prima, dada la disponibilidad potencial de estos

  19. Ecuación para el cálculo del volumen del árbol para Pinus occidentalis Sw., en el plan sierra, República Dominicana

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    J. M. Montalvo Guerrero

    2001-01-01

    Full Text Available La inminente alza de los costos de la materia prima que es utilizada por la industria forestal dominicana requiere ineludiblemente que la actividad forestal de hoy cuente con tablas de volumen, capaces de estimar con la mayor exactitud posible, el volumen de los árboles y de rodales que proveen esa materia prima. La exactitud en la estimación es imprescindible para que un buen proceso de planificación racional de manejo de bosques ocurra. En este estudio se presenta una ecuación para estimar el volumen y posteriormente elaborar la tabla de cubicación de la madera en pie de los árboles de Pinus occidentalis, Sw., utilizados por el Plan Sierra y provenientes del manejo para la reordenación de los bosques naturales de su área de influencia. Se compara la ecuación obtenida en el estudio, con la generada por (Gil y Cuevas, 1986 y utilizada para la construcción de la tabla de cubicación que se utiliza desde hace 14 años. Se pretende, si los estadígrafos así lo indican, sustituir la tabla existente ya que las muestras para ésta fueron tomadas en rodales cuyos árboles poseen características específicas y difieren en su desarrollo (son más pequeños y de menor grosor de los del resto del área de influencia del Plan Sierra, ya que fueron tomadas en una zona de vida con características edáficas y climatológicas diferentes al resto del área de influencia del Plan Sierra. La muestra para la tabla existente fue de 40 árboles. Para el presente estudio se muestrearon 191 árboles representativos de las tres diferentes zonas de vida que ocurren en La Sierra.

  20. Propiedades del suelo y productividad de Pinus taeda L. en la Mesopotamia Argentina

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    Rodolfo Andrés Martiarena

    2014-05-01

    Full Text Available Nuestro objetivo fue establecer la vinculación entre  algunas características edáficas y el crecimiento de P. taeda, en su zona de plantación comercial en la Mesopotamia Argentina. La hipótesis es que la causa principal de la reducción de la productividad de las plantaciones es la profundidad de suelo y el volumen efectivo aprovechable por las plantas, y no el contenido de nutrientes. El trabajo se realizó entre los 28º 30’ S, en la provincia de Corrientes, hasta los 25º 30’ S, en la provincia de Misiones. Se seleccionaron 31 sitios donde, 1- se tomaron muestras de suelo para determinar nutrientes y densidad aparente, 2- se midieron las variables dasométricas de las plantaciones y se calculó el Índice de Sitio (IS, 3- se tomaron muestras foliares y se analizaron las concentraciones de C, N y P. El análisis estadístico se realizó con INFOSTAT 2.0. Los sitios se asignaron a tres grupos edafoclimáticos: 1 sitios con suelos rojos del Norte, 2 sitios con suelos pedregosos del Norte y, 3 sitios con suelos rojos del Sur. El crecimiento de las plantaciones de P. taeda fue menor (menor IS en los suelos pedregosos, con más nutrientes, una menor profundidad efectiva y un  menor volumen ocupado por la fracción fina. En la zona norte, la mayor oferta de nutrientes en los suelos pedregosos (respecto de los rojos, no resultó en un aumento en la concentración de nutrientes foliares. La menor capacidad de retención de agua y un menor volumen a ser explorado por las raíces de las plantas, constituyeron la principal limitante al crecimiento en los suelos pedregosos. A pesar de las diferencias en precipitación, todos los sitios correspondieron a la misma Zona de Vida Bosque Subtropical húmedo. Entre los suelos rojos profundos, de similar concentración y contenido de nutrientes, la zona Sur resultó la más favorable para el crecimiento (mayor IS. Esta respuesta en el menos lluvioso sur  puede relacionarse con una mayor demanda atmosf

  1. Fenología del anillo de crecimiento de Pinus uncinata Ramond y Pinus sylvestris L. en un gradiente altitudinal en los Pirineos Centrales

    Directory of Open Access Journals (Sweden)

    Camarero, J. Julio

    1996-12-01

    Full Text Available In this study, we describe tree-ring growth and structure of Pinus uncinata Ram. and Pinus sylvestris L. along an altitudinal gradient in the Central Pyrenees. We measured monthly ring width increase and the number of cells added during 1993. Both species formed most of the ring from mid june to mid July 1993. Latewood development -formation, darkening and lignification of cell walls- starts in July and ends in October. The ring begins before its development in P. sylvestris than in P. uncinata that lives at higher elevation. P. uncinata formed narrower rings with less cells and greater color heterogeneity in the latewood than P. sylvestris. There is a high variability between trees in the earlywood and latewood cells position in the ring. The different phenological patterns of tree-ring growth along the selected altitudinal gradient could be partially explained by the influence of different climatic conditions, mainly thermic.

    [es] En el presente trabajo, describimos el desarrollo y la estructura del anillo anual de crecimiento de Pinus uncinata Ramondy Pinus sylvestris L. en un gradiente altitudinal en los Pirineos centrales. Medimos mensualmente el aumento de la anchura del anillo y el número de traqueidas añadidas a lo largo de 1993. En ambas especies, la mayor parte del anillo se forma desde mediados de jimio hasta mediados de julio de 1993. El desarrollo de la madera tardía -formación, coloración y engrasamiento y lignificación de las paredes celulares- comienza en julio y acaba en octubre. El anillo comienza antes su formación en los bosques estudiados de P. sylvestris que en P. uncinata, el cual vive a mayor altitud. P. uncinata forma anillos más estrechos, con menor múmero de células y con mayor heterogeneidad de color de la madera tardía que P. sylvestris. Existe una gran variabilidad entre árboles en la posición de las células de

  2. Pinus Culminicola Andresen y Beaman y sus asociaciones en la ladera sur del cerro La Viga, Coahuila

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    Rubén Sánchez Silva

    1990-01-01

    Full Text Available Pinus culminicola was described in 1961 with samples from the Cerro Potosí, State of Nuevo León, Mexico. The species was reported in the Sierras La Marta in 1962, and San Antonio de las Alazanas in 1975, State of Coahuila, indicating that there were not pure associations. In 1979 the species was colected in the Sierra La Viga, Coahuila, at 3 300 m, without data on environmental conditions in which it grows. The distribution of  P. culminicola is restricted to the south slope of the Cerro La Viga. The associations identified and described in this paper are: (1 Scrub of Quercus rugosa – Quercus durifolia – Cercocarpus montanus with elements of P. culminicola; (2 Pinus montezumae–Pseudotsuga macrolepisy with elements of P. culminicola, Quercus spp. and Arbutus xalapensis (3 P. culminicola– Quercus rugosa (both of them as shrubs; (4 P. montezumac–P. macrolepis–Pinus ayacahuite–P. culminicola; (5 pure scrub of P. culminicola; (6 P. culminicola– Dasylirion tezanum (sometimes with Arctostaphylos pungens and Quercus spp., shrublike; (7 Pinus hartwegii–P. culminicola; (8 P. hartwegii with elements of P. macrolepis and P. culminicola; and (9 induced prairie. There are differences in dominance of the species in accordance to the particular characteristics of each place, with presence of herbaceous from high altitudes. The altitude at which these associations occur, varies from 2 900 m to 3 700 m, although isolated elements of P. culminicola–were found at 2 700 m.

  3. In vitro micrografting of Sterculia setigera Del.

    African Journals Online (AJOL)

    Jane

    2010-12-13

    incompatibility in higher. Plants. Am. J. Bot. 7(5): 752-758. Onay A, Pirinc V, Adiyaman F, Isikalan C, Tilkat E, Basaran D (2002). In vivo and in vitro micrografting of Pistachio, Pistacia vera L. cv. Siirt. Turk J. Biol. 27: 95-100.

  4. Distribución potencial del Pinus martinezii: un modelo espacial basado en conocimiento ecológico y análisis multicriterio Potential distribution of Pinus martinezii: an spatial model based in ecological knowledge and muticriteria analysis

    Directory of Open Access Journals (Sweden)

    Óscar Leal-Nares

    2012-12-01

    Full Text Available El modelado de la distribución potencial y actual de las especies se ha convertido en un área de investigación muy activa; generalmente se basa en el concepto de nicho ecológico y se apoya en el uso de programas de cómputo. El objetivo principal de esta investigación fue elaborar un modelo de distribución potencial de Pinus martinezii en la cuenca del lago de Cuitzeo, utilizando información ambiental y datos de presencia de la especie, lo que requirió identificar los factores ambientales que determinan la distribución de P. martinezii, y elaborar un perfil bioclimático de la especie. El modelo se apoyó en un análisis multicriterio dentro de un sistema de información geográfica. Los atributos se agruparon en 3 criterios: geopedológicos, morfométricos y climáticos. De acuerdo con el mapa que se obtuvo, en la cuenca hay 2 zonas principales de distribución potencial de P. martinezii y algunas regiones aisladas donde no se encontraron poblaciones. El modelo espacial constituye una herramienta importante para planificar labores de conservación y reforestación; asimismo, puede utilizarse para planificar exploraciones en busca de nuevas poblaciones de P. martinezii que no han sido registradas, o identificar sitios donde esta especie pueda reintroducirse.The modeling of potential and current distribution of species has become a very active research area. Generally, modeling is based on the concept of ecological niche, and is supported by the use of computer programs. The main objective of this project was to develop a potential distribution model of Pinus martinezii in the Cuitzeo Lake basin using data of environmental variables, and presence of the target species. To this purpose, the environmental factors that determine the distribution of P. martinezii were identified, and a bioclimatic profile of the species was made. The modeling was based on a spatial multicriteria analyisis. The attributes were grouped into 3 criteria

  5. Atributos ecológicos para el manejo de Pinus tropicalis Morelet en la localidad de Galalón, Pinar del Río

    OpenAIRE

    Valdés Sáenz, María Adela

    2013-01-01

    En esta investigación se evaluaron los atributos ecológicos de la población natural de Pinus tropicalis Morelet en Galalón. Se establecieron seis parcelas de 400 m2 para la caracterización del suelo, clima, vegetación, estado de conservación, estructura florística y los atributos morfofisiológicos de la especie. La riqueza florística resultó con 30 géneros, 22 familias botánicas y 31 especies, siendo un área pobre florísticamente donde predomina P. tropicalis asociado con P. caribaea var. car...

  6. INFLUENCIA DEL USO DE REGULADORES DE CRECIMIENTO SOBRE BROTES VEGETATIVOS Y NÚMERO DE ESTRÓBILOS MASCULINOS EN Pinus pinea L. EN CHILE

    Directory of Open Access Journals (Sweden)

    Alejandro Venegas-González

    2016-01-01

    Full Text Available La utilización de reguladores de crecimiento o fitorreguladores, es una alternativa para aumentar la floración en la familia Pinaceae. En este estudio se analizaron los efectos de la aplicación de reguladores de crecimiento (fitorreguladores sobre brotes vegetativos y estróbilos masculinos de Pinus pinea L. Los productos seleccionados fueron: giberelina cuatro más siete (GA 4+7 , ácido naftalenacético (NAA y bencilaminopurina (BAP, distribuidos en siete tratamientos, incluido el control. Estos fitorreguladores se emplearon en una plantación de Pinus pinea de 16 años de edad ubicada en Toconey, comuna de Pencahue, región del Maule-Chile. Los tratamientos se aplicaron en cinco fechas, cada dos semanas (fin de agosto a inicio de noviembre. Al final de las aplicaciones se evaluaron las variables: estróbilos masculinos, número de brotes vegetativos y de nuevas brotes, y longitud de brotes apicales . Los principales resultados indicaron que la aplicación de 1.000 mg L -1 de GA 4+7 , fue el tratamiento que indujo la mayor floración masculina, presentando un 99,6 % más de estróbilos con respecto a las ramas testigo y también tuvo un efecto significativo en la longitud de los brotes apicales, aumentando más del doble la longitud respecto al testigo. En el número de brotes vegetativos, la aplicación de 500 mg L -1 BAP mostró diferencias significativas, obteniendo un incremento de 414,3 % con respecto al tratamiento control.

  7. Influencia del azulado (mancha azul) en la densidad y estabilidad dimensional de la madera de Pinus sylvestris

    OpenAIRE

    Rey-Prieto,Adrián; Riesco-Muñoz,Guillermo

    2012-01-01

    La madera de Pinus sylvestris tiene interés comercial aunque su valor se reduce considerablemente cuando presenta hongos de azulado. En el presente estudio se apearon siete pinos procedentes de plantaciones españolas, y de los fustes se muestrearon 14 rodajas a diferentes alturas. Cuando éstas estaban parcialmente azuladas se labraron para obtener probetas pequeñas sin defectos anatómicos, sobre las que se determinó la densidad y además la estabilidad dimensional en volumen y en las tres dime...

  8. Red Dendrocronológica del Pino de Altura (Pinus hartwegii Lindl. para estudios dendroclimáticos en México

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    Lorenzo Vázquez Selem

    2014-01-01

    Full Text Available El bosque de Pinus hartwegii constituye el límite superior arbóreo en las montañas de México. En este estudio se desarrolló una red de cronologías de esta especie, localizadas en volcanes del Eje Neovolcánico Transversal, en el centro del país, y picos elevados de la Sierra Madre Oriental, en el Noreste. El Análisis de Componentes Principales integró las cronologías en dos grupos, uno para el centro y otro para el Noreste, con los que se desarrollaron dos cronologías regionales de 320 años (1690-2009 y 590 años (1420-2009, respectivamente. El fenómeno de El Niño Oscilación del Sur (ENSO en su fase cálida (El Niño y fría (La Niña, mostró un impacto significativo en el comportamiento de la variabilidad hidroclimática descrita por ambas series. La Niña produjo condiciones climáticas contrastantes, es decir, secas en el Noreste y húmedas en el centro, mientras que la fase de El Niño originó sequías en ambas regiones, pero sólo durante eventos intensos de ENSO.

  9. Crescimento in vitro de isolados de Armillaria sp. obtidos de Pinus elliottii var: elliottii sob várias temperaturas In vitro growth of Armillaria sp. isolates obtained from Pinus elliottii var: elliottii under several temperatures

    Directory of Open Access Journals (Sweden)

    Nei Sebastião Braga Gomes

    2007-06-01

    Full Text Available A armilariose tem sido considerada a principal doença em Pinus no Brasil. Os sintomas e danos consistem no amarelecimento de acículas, declínio, podridão de raízes, exsudação de resina e morte. A temperatura é um dos fatores ambientais que influencia patógenos, doença de plantas ou ambos. Este trabalho avaliou o comportamento de três isolados de Armillaria sp. obtidos de P. elliottii var. elliottii, submetidos a uma faixa de temperatura de 16 a 26 ºC, utilizando a biomassa seca produzida em meio líquido como parâmetro de análise. Verificou-se que todos os isolados apresentaram máxima produção de biomassa a 22 ºC. Utilizando-se de regressão cúbica encontrou-se temperaturas de máximo crescimento entre 21,79 e 23,19 ºC. De acordo com os resultados, a melhor temperatura para crescimento dos isolados testados situou-se em 22 ºC.Armillaria root rot is the major pine disease in Brazil. Symptoms of this disease are yellowing of the needles, decline, root rot, resin exsudation and plant death. Temperature is an environmental factor that affects pathogens, the disease or both. This work evaluated the behaviour of three isolates of Armillaria sp. obtained from P. elliottii var. elliottii. The fungus isolates were submitted to temperature ranging from 16 to 26 ºC, by using dry biomass production in liquid medium as a measureable variable. All isolates produced higher amount of biomass at 22 ºC. A cubic regression showed a maximum point of temperatures between 21,8 e 23,2 ºC. The best temperature for fungus isolates growth was around 22 ºC.

  10. MicroRNAs, polyamines, and the activities antioxidant enzymes are associated with in vitro rooting in white pine (Pinus strobus L.).

    Science.gov (United States)

    Fei, Yunjun; Xiao, Bo; Yang, Man; Ding, Qiong; Tang, Wei

    2016-01-01

    Molecular mechanism of in vitro rooting in conifer is not fully understood. After establishment of a regeneration procedure in eastern white pine (Pinus strobus L.) using mature embryos as explants to induce shoot formation on medium containing 3 μM IAA, 6 μM BA and 6 μM TDZ and induce root formation on medium containing 0.001-0.05 μM IAA, 0.001-0.05 μM IBA, 0.001-0.05 μM TDZ, we have investigated the changes of polyamine content and the activities of antioxidant enzymes during in vitro rooting in P. strobus. Our results demonstrated that putrescine (Put), spermidine (Spd), and spermine (Spm) did not increase in P. strobus during the first week of rooting on medium supplemented with 0.01 μM indole-3-acetic acid (IAA), whereas the levels of Put, Spd, and Spm increased during the 1st-3rd week of culture on medium with IAA, and then decreased on medium with IAA. No such a change in Put, Spd, and Spm was observed on medium without IAA. Measurement of antioxidant enzyme activity demonstrated that the activities of polyphenol oxidase, catalase, and peroxidase slightly increased in the first week of culture and reached to the highest peak in the 3rd-5th week of culture. Quantitative RT-PCR results indicated that miR160 was increased on the 7th day, miR162, miR397, and miR408 was increased from the 21th to 35th day, miR857 was increased on the 35th day, and miR827 was increased on the 49th day. These results demonstrated that enhanced polyamine biosynthesis, antioxidant enzyme activity, and microRNAs are correlated with the root induction and formation in P. strobus.

  11. Expresión génica y dinámica de metilación del ADN en procesos de organogénesis adventicia en Pinus radiata D. Don

    OpenAIRE

    Rodríguez Lorenzo, José Luis

    2014-01-01

    Las coníferas representan más del 50 % de la masa forestal del planeta y en particular en España, el 94,4 % de este área pertenece al género Pinus teniendo a Pinus radiata D. Don como la tercera especie más importante. Debido a que los bosques juegan un papel crucial en el cambio climático, han sido incluidos en el protocolo de Kyoto por su capacidad de acumulación de CO2, considerada incluso mayor que la de la atmósfera. Para incrementar la producción, la multiplicación de genotipos élite a ...

  12. Fijación química del preservante CCA-C en la madera de Pinus elliottii Parte 2: Cinética y modelización

    OpenAIRE

    Wottitz,Carlos A; Moreno,Graciela A

    2013-01-01

    En este trabajo se analiza la cinética química global del proceso de fijación del CCA en la madera de Pinus elliottii (Engelm.) y se modeliza la evolución del proceso de fijación en función del tiempo a diferentes combinaciones de temperatura y humedad relativa. Para ello se utilizaron probetas cúbicas de 2 cm de arista, se impregnaron mediante un proceso vacío-presión con solución de CCA-C al 2 % en masa y se trataron en cámara climatizadora según nueve combinaciones de temperatura y humedad...

  13. EVALUACIÓN DEL RESIDUO DE CÁSCARA DE NUEZ (Juglans regia L. EN LA PRODUCCIÓN DE PLÁNTULAS DE Pinus patula, EN VIVERO

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    Omar Romero-Arenas

    2012-01-01

    Full Text Available Para producir plantas de calidad en vivero, se utilizan sustratos comerciales como el Peat Moss, agrolita y vermiculita, cuyo costo es elevado y reduce significativamente los márgenes de utilidad; por lo anterior, se necesita buscar sustratos alternativos. La cáscara de nuez (Juglans regia L. es un residuo sin aprovechamiento en producción; sin embargo, cuenta con importantes elementos nutritivos para ser aprovechados por los organismos vegetales. Se evaluó el crecimiento inicial de Pinus patula producido en vivero, con composta de cáscara de nuez con agrolita y vermiculita, mediante sustitución paulatina del Peat Moss. Con un diseño experimental completamente al azar, se estudiaron 4 tratamientos a base de cáscara de nuez + agrolita y vermiculita, en proporciones que variaron desde 0% hasta 80% de cáscara de nuez, y la mezcla de sustratos comerciales. A los 7 meses y medio, las plántulas que se desarrollaron en el tratamiento 1, mezcla testigo (Peat Moss 33% + vermiculita 33% + agrolita 33% y el tratamiento 4 (Cáscara de nuez 33% + vermiculita 33% + agrolita 33% presentaron valores más altos en las variables: altura, diámetro, peso seco aéreo, peso seco radicular y peso seco total. La relación aérea/raíz y el índice de esbeltez no presentaron diferencias entre los 4 tratamientos. El valor más alto del índice de calidad de Dickson fue para el testigo (0,25 g.cm-1, seguido por el tratamiento 4 (0,24 g.cm-1, sin diferencias estadísticas. La cáscara de nuez permite producir plántulas sanas, por lo que es útil como sustrato alternativo para la producción de plantas en vivero, lo que reduce los costos de producción además de contribuir con el sector productivo forestal.

  14. Comparación de la eficacia de distintos productos químicos aplicados mediante tratamiento aéreo en el control del muérdago (Viscum album) sobre Pinus halepensis

    OpenAIRE

    Perdiguer Brun, Agustín; Cañada Martín, J. F.; Fernández López, F.; Colinas, C.

    2001-01-01

    El objetivo de este trabajo fue comparar la eficacia de diversos productos químicos en el control del muérdago (Viscum album var austriacum) sobre Pinus halepensis. La experiencia consistió en la aplicación mediante tratamiento aéreo de diversas concentraciones de etefon, ácido giberélico y glifosato. Las combinaciones de ácido giberélico y glifosato obtuvieron las mayores eficacias, especialmente la que aplicó 7,8 g/ha de ácido giberélico y 540 g/ha de glifosato. The aim of this wor...

  15. Análisis de los impactos en la calidad del suelo causados por el Pino (Pinus patula) en comparación con el suelo ocupado por Polylepis (Polylepis reticulata) en el Parque Nacional Cajas

    OpenAIRE

    Mancheno Herrera, Andrea Cecilia

    2011-01-01

    El trabajo de investigación que se presenta consiste en realizar la identificación de las zonas con Pinus patula en el Parque Nacional Cajas mediante georeferenciación y la posterior transferencia de datos al software para su interpretación, a continuación, en base a las variables pendiente y altitud se procede a escoger los lugares para la toma de muestras y poder evaluar si este tipo de vegetación está o no alterando las características naturales del parque. Para esto se escoge la altitud d...

  16. REDUCCIÓN DE LA BIOMASA DEL PINO CARRASCO (PINUS HALEPENSIS EN UN ÁREA DEL SURESTE SEMIÁRIDO PENINSULAR COMO ESTRATEGIA PARA EVITAR EL ESTRÉS HÍDRICO

    Directory of Open Access Journals (Sweden)

    Francisco Belmonte Serrato

    2008-01-01

    Full Text Available En las épocas de fuertes sequías, la vegetación mediterránea sufre un estado de estrés hídrico prolongado que le obliga a adoptar diversas estrategias de supervivencia, entre las que fi guran, como último recurso, y antes de llegar a superar su capacidad de resilencia, la reducción de la biomasa de hojas. En este trabajo se pone de manifi esto como algunos ejemplares de Pinus halepensis existentes en el área de estudio (el campo experimental de El Ardal, Murcia, redujeron apreciablemente su biomasa aérea en respuesta a la sequía, que se manifestó en un importante aumento de la producción de hojarasca. La producción de hojarasca se relaciona además de con la precipitación registrada en el periodo de estudio, con la humedad del suelo, parámetro clave en la supervivencia de la vegetación en ambientes secos.

  17. Pinus L. Pine

    Science.gov (United States)

    Stanley L. Krugman; James L. Jenkinson

    1974-01-01

    Growth habit, occurrence, and use. The genus Pinus, one of the largest and most important of the coniferous genera, comprises about 95 species and numerous varieties and hybrids. Pines are widely distributed, mostly in the Northern Hemisphere from sea level (Pinus contorta var. contorta) to timberline (P...

  18. Ethanol Extract of Pinus koraiensis Leaf Ameliorates Alcoholic Fatty Liver via the Activation of LKB1-AMPK Signaling In Vitro and In Vivo.

    Science.gov (United States)

    Hong, Sang-Hyuk; Lee, Hyemin; Lee, Hyo-Jung; Kim, Bonglee; Nam, Min-Ho; Shim, Bum-Sang; Kim, Sung-Hoon

    2017-05-01

    Although Pinus koraiensis leaf (PKL) was reported for its anti-diabetes, anti-obesity and anticancer effects as a folk remedy, the inhibitory effect of PKL on alcoholic fatty liver has never been elucidated yet. This study investigated the molecular mechanisms of PKL on alcoholic fatty liver in HepG2 cells, Sprague Dawley (SD) rats and Imprinting Control Region (ICR) mice. Pinus koraiensis leaf increased phosphorylation of liver kinase B1 (LKB1)/AMP-activated protein kinase signaling, low-density lipoprotein receptor and decreased fatty acid biosynthesis-related proteins such as sterol regulatory element-binding protein 1c, fatty acid synthase, 3-hydroxy-3-methylglutaryl-CoA reductase in HepG2 cells. In SD rats with 25% alcohol-induced fatty liver, PKL suppressed the levels of aspartate aminotransferase and triglyceride and also enhanced the activities of antioxidant enzymes including superoxide dismutase, glutathione peroxidase and glutathione s-transferase compared with untreated control. Furthermore, PKL increased serum alcohol dehydrogenase and serum aldehyde dehydrogenase, but decreased serum alcohol concentration in ICR mice after alcohol administration. Consistently, histochemical analysis revealed that PKL attenuated alcohol-induced fatty liver in SD rats. Overall, these findings suggest that PKL ameliorates alcohol-induced fatty liver via activation of LKB1-AMP-activated protein kinase and modulation of proteins related to lipogenesis synthesis, cholesterol synthesis and fatty acid oxidation. Copyright © 2017 John Wiley & Sons, Ltd. Copyright © 2017 John Wiley & Sons, Ltd.

  19. Mineralización del nitrógeno, carbono y actividad enzimática del suelo en un bosque de Nothofagus obliqua (Mirb Oerst y una plantación de Pinus radiata D. Don. del centro-sur de Chile Nitrogen and carbon mineralization and enzyme activity in soils of Nothofagus obliqua (Mirb Oerst stands and Pinus radiata D. Don plantation in south-central Chile

    Directory of Open Access Journals (Sweden)

    YESSICA RIVAS

    2009-03-01

    Full Text Available En Chile, el establecimiento de plantaciones comerciales de rápido crecimiento ha sido sostenido en las últimas décadas mediante la sustitución de bosques nativos y conversión de suelos agrícolas. Pinus radiata D. Don es la principal especie productiva, debido a su crecimiento acelerado y adaptabilidad al clima y los suelos. En el presente estudio se plantea que la actividad biológica del suelo es variable a través del año, en respuesta a variaciones de precipitación, temperatura y contenido de humedad de suelo y que el cambio de uso de suelo desde un bosque templado de Nothofagus obliqua (Mirb Oerst a una plantación con coniferas exóticas, modifica la química del suelo y consecuentemente los procesos de N-min, C-min y la actividad biológica del suelo. Esta hipótesis fue examinada en un bosque de N. obliqua y una plantación de P. radiata del centro-sur de Chile (40°07' S, 72° O. Se evaluó mensualmente la tasa mineralización de nitrógeno (N-min, cabono (C-min y la actividad enzimática potencial del suelo (ureasa, proteasa e hidrólisis de la fluoresceína diacetato entre septiembre 2003 y mayo 2005. Los resultados demuestran que los niveles de las variables de actividad biológica del suelo fueron significativamente diferentes entre las parcelas de bosque y plantación (Lambda de Wilk = 0,022; F 5,80 = 733; P In Chile, commercial forests plantations have increased during the last decades due in part to replacement of native forests and conversion of agricultural soils. Pinus radiata D. Don has been the main tree planted, due to its rapid growth and adaptability. In the present study we proposed that biological activity varies along the year due to changes of precipitation, temperature and soil water content and mainly because the conversion of native forest to exotic P. radiata plantations alters the soil chemistry, N and C mineralization and the potential enzymatic activity in these soils. This hypothesis was examined in a

  20. Appraisal on the wound healing and anti-inflammatory activities of the essential oils obtained from the cones and needles of Pinus species by in vivo and in vitro experimental models.

    Science.gov (United States)

    Süntar, Ipek; Tumen, Ibrahim; Ustün, Osman; Keleş, Hikmet; Akkol, Esra Küpeli

    2012-01-31

    According to ethnobotanical data, Pinus species have been used against rheumatic pain and for wound healing in Turkish folk medicine. Essential oils from the cones and needles of five different Pinus species (Pinus brutia Ten., Pinus halepensis Mill., Pinus nigra Arn., Pinus pinea L. and Pinus sylvestris L.) were evaluated for their in vivo wound healing and anti-inflammatory activities. In vivo wound healing activity of the ointments prepared from essential oils was evaluated by linear incision and circular excision experimental wound models subsequently histopathological analysis and hydroxyproline content. Furthermore, the essential oils were screened for anti-hyaluronidase activity. Additionally anti-inflammatory activity was assessed by using the method of Whittle, which is based on the inhibition of acetic acid-induced increase in capillary permeability. The essential oils obtained from the cones of Pinus pinea and Pinus halepensis demonstrated the highest effects on the wound healing activity models. On the other hand, the rest of the essential oils did not show any significant wound healing and anti-inflammatory activities. The experimental study revealed that essential oils obtained from the cones of Pinus pinea and Pinus halepensis display remarkable wound healing activity. Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

  1. Contenido de carotenos en el follaje de Pinus caribaea Morelet y Pinus tropicalis Morelet

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    Rolando Quert Álvarez

    1997-08-01

    Full Text Available Se realizó un análisis del material vegetal de las especies Pinus caribaea Morelet y Pinus tropicalis Morelet, con el objetivo de determinar su contenido de caroteno tomando como base las condiciones y tiempo de exposición del follaje de las especies objeto de estudio al sol y a la sombra, y teniendo en cuenta la extracción del aceite esencias como factores influyentes en la variación de las concentraciones de caroteno. Para determinar el contenido de caroteno se tomaron muestras del follaje entre 1 y 20 d, expuesto a las condiciones de trabajo en intervalos de 1, 3, 6, 10 y 20 d, tanto antes como después de extraer el aceite esencial. Los resultados obtenidos para ambas especies demostraron que el follaje expuesto a la sombra contiene un mayor porcentaje de caroteno que el expuesto al sol, como era de esperar; el tiempo de exposición influye significativamente en este contenido, así como la extracción del aceite esencial que aumenta el contenido de caroteno; los valores máximos fueron de 130,7 y 157,2 mg/kg de follaje y los mínimos de 55,3 y 57,2 mg/kg de follaje para Pinus caribaea Morelet y Pinus tropicalis Morelet respectivamente.An analysis of the vegetable material from the species Pinus cariabaea Morelet and Pinus tropicalis Morelet was carried out and their content of carotene was determined taking as the basis the conditions and time of exposure of the foliage of the species studied to the sun and shade, and also taking into account the extraction of the essential oil as factors influencing on the variation of carotene concentrations. For the determination of the carotene content, samples of the foliage between 1 and 20 exposed to working conditions at intervals of 1, 3, 6, 10 and 20 d were taken, both before and after the extraction of the essential oil. Results obteined from both species showed that the foliage exposed to shade contains a higher percentage of carotene than the one exposed to the sun, as it was expected to

  2. Endophytic Bacteria from Pinus taeda L. as Biocontrol Agents of Fusarium circinatum Nirenberg & O'Donnell Bacterias Endófitas de Pinus taeda L. como Agentes de control Biológico de Fusarium circinatum Nirenberg & O'Donnell

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    Silvina Soria

    2012-06-01

    Full Text Available Fusarium circinatum Nirenberg & O'Donnell, the pitch canker fungus, has been recently reported in Uruguay affecting Pinus taeda L. seedlings. The spread of this pathogen to plantations constitute a risk to forestry production. The aim of this work was to evaluate the inhibitory effect of live bacteria and their thermostable metabolites on F. circinatum growth in vitro. Four Bacillus subtilis strains and one of Burkholderia sp. isolated as P. taeda endophytes were evaluated as biological control agents of F. circinatum. Dual cultures between live bacteria and pathogen were performed. Furthermore, bacteria metabolites obtained from liquid cultures were sterilized and added to the culture media where fungus was grown. In this study all bacteria showed an antagonist effect on the pathogen growth arresting the mycelia at one cm of the edge of the bacteria colony. Bacteria thermostable metabolites reduced over 50% fungal growth. These results demonstrates that endophytic bacteria, well adapted to live in host tissues, constitute a good alternative to control F. circinatum affecting Pinus seedlings.La presencia de Fusarium circinatum Niremberg & O'Donnell, agente causal del cancro resinoso en pino, ha sido detectada recientemente en plántulas de Pinus taeda L. en Uruguay. La propagación de este patógeno en las plantaciones constituye un riesgo para la producción forestal. El objetivo de este trabajo fue determinar la capacidad inhibitoria de bacterias vivas y de sus metabolitos termoestables sobre el crecimiento de F. circinatum in vitro. Cuatro cepas de Bacillus subtilis y una de Burkholderia sp. aisladas como endófitas de P. taeda, fueron evaluadas como potenciales agentes de control biológico sobre F. circinatum. Para ello, se realizaron enfrentamientos directos entre las bacterias vivas y el micelio del patógeno. Por otra parte, los metabolitos bacterianos obtenidos de cultivos líquidos fueron esterilizados en autoclave y se incorporaron al

  3. HONGOS COMESTIBLES SILVESTRES: ESPECIES EXÓTICAS DE SUILLUS (BOLETALES, BASIDIOMYCOTA Y LACTARIUS (RUSSULALES, BASIDIOMYCOTA ASOCIADAS A CULTIVOS DE PINUS ELLIOTTII DEL NORDESTE ARGENTINO

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    Nicolás Niveiro

    2009-01-01

    Full Text Available Los hongos silvestres comestibles son un recurso económico importante que permite obtenerun producto deshidratado que puede comercializarse fácilmente. En el presente trabajo, y conel objeto de dar a conocer este recurso, se ilustran y describen dos especies de hongosmicorrícicos asociadas a cultivos de Pinus elliottii en el nordeste argentino que no han sidomencionadas anteriormente para la región: Lactarius deliciosus para la provincia de Misionesy Suillus granulatus para las provincias de Corrientes y Misiones

  4. EVALUACIÓN DEL RESIDUO DE CÁSCARA DE NUEZ (Juglans regia L.) EN LA PRODUCCIÓN DE PLÁNTULAS DE Pinus patula, EN VIVERO

    OpenAIRE

    Omar Romero-Arenas; Romelia López Escobedo; Miguel Á. Damián Huato; Israel Hernández Treviño; José F. Parraguirre Lezama; Manuel Huerta Lara

    2012-01-01

    Para producir plantas de calidad en vivero, se utilizan sustratos comerciales como el Peat Moss, agrolita y vermiculita, cuyo costo es elevado y reduce significativamente los márgenes de utilidad; por lo anterior, se necesita buscar sustratos alternativos. La cáscara de nuez (Juglans regia L.) es un residuo sin aprovechamiento en producción; sin embargo, cuenta con importantes elementos nutritivos para ser aprovechados por los organismos vegetales. Se evaluó el crecimiento inicial de Pinus pa...

  5. Pinus Culminicola Andresen y Beaman y sus asociaciones en la ladera sur del cerro La Viga, Coahuila

    OpenAIRE

    Rubén Sánchez Silva; José López García; José Manuel Espinoza Rdz.

    1990-01-01

    Pinus culminicola was described in 1961 with samples from the Cerro Potosí, State of Nuevo León, Mexico. The species was reported in the Sierras La Marta in 1962, and San Antonio de las Alazanas in 1975, State of Coahuila, indicating that there were not pure associations. In 1979 the species was colected in the Sierra La Viga, Coahuila, at 3 300 m, without data on environmental conditions in which it grows. The distribution of  P. culminicola is restricted to the south slope of the Cerro La V...

  6. Recombinación del VIH-1 in vitro

    OpenAIRE

    Iglesias Sánchez, María José

    2003-01-01

    Este trabajo se ha centrado en el estudio de la influencia que ejerce la recombinación en la evolución de virus de la inmunodeficiencia humana (VIH), ya que se trata del principal mecanismo de variaciones este virus. En primer lugar se realizaron una serie de infecciones dobles con diferencias combinaciones de dos cepas del VIH-1, en células MT4 (línea linfocítica humana), que se mantuvieron en cultivo durante 22 días. Los ADNs procedentes de varios días a lo largo de los cultivos se analizar...

  7. In vitro micrografting of Sterculia setigera Del. | Niang | African ...

    African Journals Online (AJOL)

    An in vitro procedure micrografting of adult scions of Sterculia setigera was developed to overcome low rooting in adult shoot. Axenic micro shoots of 0.5 cm length taken from adult trees as scions were grafted on seedlings rootstocks cultured on MS medium. 100% success was obtained with micrografts using adult apex as ...

  8. In vitro ectomycorrhizal specificity between the Asian red pine Pinus densiflora and Tricholoma matsutake and allied species from worldwide Pinaceae and Fagaceae forests.

    Science.gov (United States)

    Yamada, Akiyoshi; Kobayashi, Hisayasu; Murata, Hitoshi; Kalmiş, Erbil; Kalyoncu, Fatih; Fukuda, Masaki

    2010-06-01

    Tricholoma matsutake produces commercially valuable, yet uncultivable, mushrooms (matsutake) in association with pines in the Far East and Scandinavia and with both pines and oaks in the foothills of Tibet. Other matsutake mushrooms, such as Tricholoma anatolicum from the Mediterranean regions and Tricholoma magnivelare and Tricholoma sp. from the North Pacific Coast area of Canada and North America as well as Mexico, respectively, are associated with pines or oaks in their natural habitats. Tricholoma bakamatsutake and Tricholoma fulvocastaneum from Asia produce moderately valuable matsutake mushrooms and are solely associated with Fagaceae in nature. In this study, we demonstrate for the first time that matsutake mushrooms from Scandinavia, Mediterranean regions, North America, and Tibet form ectomycorrhizae with Pinus densiflora similar to the Far East T. matsutake. In general, worldwide T. matsutake and the symbionts of Pinaceae colonize the rhizospheres of P. densiflora as well as T. matsutake isolated from the host plant. However, T. fulvocastaneum and T. bakamatsutake formed a discontinuous Hartig net and no Hartig net, respectively, and colonized to a lesser extent as compared to T. matsutake. The data suggest that conifer-associated matsutake mushrooms in their native habitat will associate symbiotically with the Asian red pine.

  9. Historia del cultivo in vitro en España

    OpenAIRE

    Ballester, Antonio

    2007-01-01

    Conferencia inaugural de la VII Reunión Nacional de la Sociedad Española de Cultivo In Vitro de Tejidos Vegetales (SECIVTV).-- Publicado en el Libro de resúmenes de la VII Reunión de la SECIVTV (Alcalá de Henares, 25-27 Junio 2007). Editores: M. Toribio, M.C. Sánchez, J.M. González, J. Alegre.

  10. CURVAS ANAMÓRFICAS DE ÍNDICE DE SITIO PARA PLANTACIONES DE Pinus caribaea Morelet var. caribaea Barret y Golfari DE LA EMPRESA FORESTAL INTEGRAL MACURIJE (EFI EN LA PROVINCIA DE PINAR DEL RÍO, CUBA

    Directory of Open Access Journals (Sweden)

    Héctor Barrero-Medel

    2011-01-01

    Full Text Available En el trabajo se seleccionó el mejor modelo para la obtención de curvas anamórficas de índice de sitio para la especie Pinus caribaea Morelet var. caribaea Barret y Golfari, en las condiciones de la Empresa Forestal Integral Macurije, perteneciente a los municipios Guane y Mantua de la provincia de Pinar del Río. Para ello se utilizó el registro de los datos de 14 parcelas permanentes establecidas en el año 2006 con mediciones consecutivas efectuadas y distribuidas en las diferentes Unidades Básicas de Producción Forestal (UBPF, 256 parcelas temporales distribuidas al azar en todo el patrimonio de la empresa y el inventario de la ordenación realizado en los años 2002-2003. El método de construcción del sistema de curvas de índice de sitio empleado mediante el principio anamórfico fue el de regresión anidada aplicado por Bailey y Clutter (1976 y descrito por Alder (1980 como método de regresión jerárquica con estimador de pendiente común. Como resultado del ajuste se obtuvieron las ecuaciones para la altura dominante (Ho para los 13,16, 19, 22, 25 y 28 metros a la edad base de 35 años, así como la ecuación para la determinación del índice de sitio.

  11. Effects of temperature, pH and carbon and nitrogen sources on growth of in vitro cultures of ectomycorrhizal isolates from Pinus heldreichii forest

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    Jelena Lazarević

    2016-04-01

    Full Text Available Aim of study: This study aims to provide basic information about physiological characteristics of isolates of Lactarius deliciosus (L. Gray, Russula sanguinaria (Schumach. Rauschert, Suillus collinitus (Fr Kuntze, Suillus granulatus (L. Rousell, Tricholoma batchii Gulden and Tricholoma imbricatum (Fr. Kumm.Area of study: The isolates are obtained from Pinus heldreichii H. Christ forest in the south-eastern part of Montenegro.Material and methods: The isolates were molecularly characterised by internal transcribed spacer (ITS sequencing and restriction fragment length polymorphism (RFLP analysis. The effects of different temperatures (20, 22, 25°C, pHs (4, 4.5, 5.2, 5.8, 6.5, 7.5, and carbon (glucose, sucrose, dextrin, arabinose, xylose and starch and nitrogen (NH4+, NO3- and protein sources on their growth were examined under laboratory conditions.Main results: The studied factors established significant differences in the development of isolates. Isolates of R. sanguinaria, L. deliciosus and both Suillus, were characterised by faster growth at 22°C, while Tricholoma isolates grew faster at 25°C. S. granulatus, S. collinitus and T. imbticatum isolates grew well at lower pH values (4 - 5.2, while L. deliciosus, R. sanguinaria and T. bachii exhibited faster growth at pHs between 5.8 and 6.5. The examined isolates were able to utilize various carbohydrates as carbon sources. The biggest mycelial growth was characterised for sucrose, then glucose, dextrin, arabinose, starch and xylose. They grew on all examined nitrogen sources, while the biggest mycelia growth was achieved on ammonium, followed by nitrate and protein. Those characteristics varied amongst the species.Research highlights: Information about physiological characteristics of Tricholoma, Lactarius, Russula, as well as Suillus, are sparse. Hence, the data obtained in this study could contribute to the understanding of their function in ecosystems. 

  12. Effects of temperature, pH and carbon and nitrogen sources on growth of in vitro cultures of ectomycorrhizal isolates from Pinus heldreichii forest

    Energy Technology Data Exchange (ETDEWEB)

    Lazarević, J.; Stojičić, D.; Keča, N.

    2016-07-01

    Aim of study: This study aims to provide basic information about physiological characteristics of isolates of Lactarius deliciosus (L.) Gray, Russula sanguinaria (Schumach.) Rauschert, Suillus collinitus (Fr) Kuntze, Suillus granulatus (L.) Rousell, Tricholoma batchii Gulden and Tricholoma imbricatum (Fr.) Kumm. Area of study: The isolates are obtained from Pinus heldreichii H. Christ forest in the south-eastern part of Montenegro. Material and methods: The isolates were molecularly characterised by internal transcribed spacer (ITS) sequencing and restriction fragment length polymorphism (RFLP) analysis. The effects of different temperatures (20, 22, 25°C), pHs (4, 4.5, 5.2, 5.8, 6.5, 7.5), and carbon (glucose, sucrose, dextrin, arabinose, xylose and starch) and nitrogen (NH4+, NO3- and protein) sources on their growth were examined under laboratory conditions. Main results: The studied factors established significant differences in the development of isolates. Isolates of R. sanguinaria, L. deliciosus and both Suillus, were characterised by faster growth at 22°C, while Tricholoma isolates grew faster at 25°C. S. granulatus, S. collinitus and T. imbticatum isolates grew well at lower pH values (4 - 5.2), while L. deliciosus, R. sanguinaria and T. bachii exhibited faster growth at pHs between 5.8 and 6.5. The examined isolates were able to utilize various carbohydrates as carbon sources. The biggest mycelial growth was characterised for sucrose, then glucose, dextrin, arabinose, starch and xylose. They grew on all examined nitrogen sources, while the biggest mycelia growth was achieved on ammonium, followed by nitrate and protein. Those characteristics varied amongst the species. Research highlights: Information about physiological characteristics of Tricholoma, Lactarius, Russula, as well as Suillus, are sparse. Hence, the data obtained in this study could contribute to the understanding of their function in ecosystems. (Author)

  13. Determinación no invasiva del sexo de embriones cultivados "in vitro"

    OpenAIRE

    Gómez, Enrique; Múñoz, M.; Simó, C.; Ibáñez, C; Carrocera, S.; Martín, D.; Cifuentes, A.

    2017-01-01

    El uso de embriones producidos in vitro permite aumentar la productividad de las explotaciones ganaderas bovinas. La transferencia de estos embriones a hembras receptoras sirve tanto para acelerar la mejora genética, por medio del nacimiento de más crías de alto mérito genético, como para paliar la infertilidad propia de las vacas de alta producción lechera. Y si se selecciona el semen o los propios embriones adecuadamente, se producen crías del sexo deseado, normalmente hembras para producir...

  14. ACTIVACION DE GENES EN LA RESPUESTA GRAVITROPICA Y PAPEL DE BIOSINTESIS Y TRANSDUCCION DE SEÑALES HORMONALES DEL ETILENO EN PINUS RADIATA D DON

    OpenAIRE

    RAMOS CAMPOS, PATRICIO EUGENIO

    2010-01-01

    La respuesta de la planta frente al estimulo gravitrópico es un fenómeno ampliamente estudiado en plantas, pero muy poco abordado en especies forestales de interés económico. Dada la importancia del cultivo de pino radiata en el sector forestal de nuestro país y el conocimiento acerca del control de la expresión génica asociado a la perturbación gravitacional, que se mantiene en gran parte aún desconocidos, hacen que el estudio del mecanismo molecular que subyace a este fenómeno cobre una gra...

  15. Evaluación in vitro del efecto erosivo de tres bebidas carbonatadas sobre la superficie del esmalte dental

    OpenAIRE

    Liñan Duran, Carlos; Facultad de Estomatológia. Universidad Peruana Cayetano Heredia. Lima,; Meneses López, Abraham; Docente del Departamento Académico de Estomatología del Niño y del Adolescente Facultad de Estomatológia. Universidad Peruana Cayetano Heredia. Lima,; Delgado Cotrina, Leyla; Docente del Departamento Académico de Clínica Estomatológica. Facultad de Estomatológia. Universidad Peruana Cayetano Heredia. Lima,

    2014-01-01

    El propósito de este estudio, in vitro, fue evaluar el efecto erosivo de tres bebidas carbonatadas sobre la superficie del esmalte dental. Se utilizaron 60 especímenes divididos en cuatro grupos de los cuales tres fueron expuestos durante un minuto a la acción de las bebidas carbonatadas,seguido por tres minutos de inmersión en saliva artificial. Este ciclo se repitió durante 20 minutos. El grupo control negativo fue inmerso en agua destilada. El efecto erosivo se evaluó mediante el método de...

  16. Supersincronización del crecimiento in vitro del Plasmodium falciparum

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    María Orfa de Rojas

    1987-12-01

    Full Text Available Se logró un crecimiento "Super sincrónico" del parásito Plasmodium falciparum en cultivo, mediante la aplicación de tres tratamientos sucesivos: sorbitol (para destruir formas maduras, Percoll (para concentrar esquizontes y nuevamente sorbitol, después de permitir la reinvación. En esta forma se obtuvo una población de parásitos con un rango de edad entre 0-3 horas, de los cuales el 99.1% fueron esquizontes a las 48 horas de incubación. El desarrollo morfológico del parásito fue completamente normal después del tratamiento y su sincronía se controló estrictamente cada 4 horas en el primer ciclo.

  17. Efecto inhibidor del crecimiento bacteriano in vitro del plasma rico en plaquetas

    OpenAIRE

    Huapaya Lazo, Carlos Enrique; Noriega Castañeda, Jorge

    2008-01-01

    Objetivo: determinar el efecto inhibidor del crecimiento bacteriano del Plasma Rico en Plaquetas (PRP) sobre el Estafilococo aureus. Material y método: se tomó 20cc de sangre a cada uno de los cinco pacientes que intervinieron en el estudio, obteniéndose el PRP activado con trombina y bovina en cada una de las muestras. Se realizaron dos procedimientos diferentes, procesándose cada muestra por quintuplicado. En el primero se enfrenta el PRP puro y combinado con Ampicilina 500mg con el Estafil...

  18. Estimation of aerial biomass using discrete-wave LiDAR data in combination with different vegetation indices in plantations of Pinus radiata (D. DON, Región del Maule, Chile.

    Directory of Open Access Journals (Sweden)

    Diego Valencia Delgado

    2014-10-01

    Full Text Available The aerial biomass of Pinus radiata plantations in the Región del Maule, Chile, was estimated from linear models using databases of LiDAR and multispectral LANDSAT ETM+. Six descriptive height variables were obtained from the LiDAR point cloud; the 25%, 50%, 75%, 95% and 100% percentiles and the mean height. Two variables associated with the density of points were also obtained, which relate the returns between fixed weighted intervals calculated as a function of the observed biomass. For multispectral variables we used NDVI, corrected NVDI (NDVIc and the “Tasseled Cap” components brilliance, greenness and humidity. The results showed coefficients of determination (R2 between 0.801 and 0.814, with errors between 36.07 and 36.11 ton ha-1 for the models generated using height percentiles, and R2 from 0.807 to 0.823 with errors between 36.06 and 36.84 ton ha-1 for transformed LiDAR data. Finally, the stepwise model using all available variables had R2 of 0.821-0.835 with errors of 34.28 - 36.31 ton ha-1.

  19. Gene expression profiles of different breast cancer cells compared with their responsiveness to fermented mistletoe (Viscum album L.) extracts Iscador from oak (Quercus), pine (Pinus), white fir (Abies) and apple tree (Malus) in vitro.

    Science.gov (United States)

    Eggenschwiler, Jenny; Patrignani, Andrea; Wagner, Ulrich; Rehrauer, Hubert; Schlapbach, Ralph; Rist, Lukas; Ramos, Mac H; Viviani, Angelika

    2006-06-01

    Cytotoxicity assays in vitro (MTT test) showed that the different breast cancer cell lines Kpl-1, MCF-7 and Mfm-223 respond differently to the mistletoe (Viscum album L.) preparations Iscador. Quercus (Qu), Abies (A), Malus (M) and Pinus (P). In order to determine the differences in the responsiveness of the cells more exactly, the gene expression profiles were determined by cells, which were treated with Mistletoe extracts, compared with untreated control cells. Such differences can be analysed in more detail by looking at the gene expression using Human Whole Genome microarray chips (41,000 genes). The results of the transcriptome analyses suggested that Iscador preparations influenced the overregulation of genes regarding immune defense, stress response, apoptosis and cell-cell adhesion pathways. Within the Mfm-223-Zellen was the Genexpression in MCF-7 and Kpl-1. The MCF-7 cells were affected on the genes which are involved in cell-cell contacts whereas Kpl-1 responded to the mistletoe extracts by changing the mRNA levels of the immune and stress response pathways. Concerning the effects of the mistletoe extract, we conclude that Iscador Qu and M have a greater influence on the immune defense and stress response genes whereas Iscador A tends to affect the cell-cell adhesion and cytoskeleton pathways. In summary, cDNA microarray analyses give us information on whether a cancer cell is sensitive to mistletoe extracts in relation to how many genes are significantly overrepresented after mistletoe treatment, and whether a particular mistletoe extract is more effective on a specific cancer cell than the other preparation.

  20. BOSQUES RELICTOS DE PINUS SYLVESTRIS L. EN LA SIERRA DE BAZA (PRDVINCIA DE GRANADA, ESPANA: ANÁLISIS Y CARTOGRAFIA DEL ESTADO ACTUAL DE LA VEGETACION

    Directory of Open Access Journals (Sweden)

    JOSÉ ANTONIO OLMEDO COBO

    2012-01-01

    Full Text Available En la Sierra de Baza (provincia de Granada, España se localiza uno de los dos núcleos de bosque endemico de pino silvestre de la provincia biogeográfica Bética. Ocupa algunas cumbres calcáreas dentro del piso bioclimático oromediterráneo subhúmedo, en enclaves que se convierten en fito-refugios al tratarse de orientaciones frescas y umbrías, acordes a los requerimientos ecológicos de la especie. Junto a estas condiciones, resulta esencial, al menos en parte, el tipo de sustrato sobre el que aparece el pinar; se trata de filitas y cuarcitas, en un entorno general de calizas y dolomías, sobre las que se desarrollan suelos de alta impermeabilidad que contrarrestan la ausencia de precipitaciones estivales. Este artículo analiza el estado actual de la fitocenosis relictual del pinar de pino silvestre en la Sierra de Baza a partir del trabajo de campo y, asimismo, presenta una cartografía de detalle de las formaciones vegetales que la componen.

  1. Estudio de la influencia del fuego en la actividad micorrícica de una masa forestal dominada por Pinus pinaster Ait.

    OpenAIRE

    López Sainz, Felicidad

    2014-01-01

    En el presente estudio se plantea el objetivo de determinar el efecto del fuego sobre la comunidad micorrícica presente en una masa forestal dominada por P. pinaster. Para ello se ha analizado el efecto de un incendio que tuvo lugar en el término municipal de Honrubia de la Cuesta. Se ha realizado sobre cuatro sustratos diferentes, con distinto grado de afectación por el fuego, fuego de alta severidad, zona de severidad media, zona no quemada y control externo con turba forestal esteriliz...

  2. Evaluación de la eficacia in vitro e in vivo del tratamiento antimicrobiano combinado por el tablero de damas

    National Research Council Canada - National Science Library

    Espino Hernández, María; Martínez Motas, Isabel; Couto Ramos, María Julia; Zuazo Silva, Jorge L

    2009-01-01

    El presente trabajo tuvo como objetivo, evaluar la eficacia clínica del tratamiento antimicrobiano combinado mediante pruebas de sinergismo in vitro y determinar la eficacia del procedimiento empleado para orientar...

  3. Análisis retrospectivo del decaimiento en Pinus sylvestris L. (Sierra de Gúdar, Teruel) mediante el análisis de la composición isotópica (13C/12C, 18O/16O) en anillos de crecimiento.

    OpenAIRE

    Carulla Montañés, David

    2010-01-01

    Al 2002 es van detectar els primers símptomes de decaïment en Pinus sylvestris, a la Serra de Gúdar (Teruel). Individus afectats i sans es trobaven barrejats. El contrast tèrmic de finals de 2001 es presumeix com a factor incitador del decaïment, degut a la manca d’enduriment i pèrdua de la conductivitat hidràulica. També es va detectar un factor de predisposició dels arbres en relació a una possible inanició del carboni sota un estrés hídric perllongat. Possiblement això va afectar prèviamen...

  4. UTILIZACIÓN DEL NEMATODO ENTOMOPATÓGENO Heterorhabditis atacamensis CIA-NE07 EN EL CONTROL DEL PICUDO DEL BANANO Cosmopolites sordidus EN CONDICIONES IN VITRO

    Directory of Open Access Journals (Sweden)

    Marianela Amador

    2015-01-01

    Full Text Available Entre las especies de barrenadores del banano, el picudo negro ( Cosmopolites sordidus es la plaga de mayor importancia económica en Costa Rica y a nivel mundial. El control de C. sordidus en sistemas intensivos de producción se basa principalmente en la aplicación de insecti - cidas, por lo que se necesita buscar alternativas biológicas, como el uso de nematodos entomo - patógenos (NEP. Se evaluó la susceptibilidad de Cosmopolites sordidus a Heterorhabditis ataca - mensis CIA-NE07. Se midió el efecto de inocular juveniles infectivos (JI de H. atacamensis sobre larvas y adultos de C. sordidus, in vitro y en cormos artificialmente infectados. Las larvas inoculadas con NEP presentaron mortalidad de 88% al segundo día y 100% al tercer día; no se observó mortalidad de adultos. Los tratamien - tos de 100, 500 y 1000 JI.larva -1 presentaron diferencias estadísticamente significativas con respecto al control y la DL50 fue de 52 JI.larva -1 . Cuando las larvas se colocaron dentro de los cormos, la DL50 aumentó a 375 JI.larva -1 . Los resultados indican que la cepa H. atacamensis CIA-NE07 es capaz de localizar e infectar larvas de picudo dentro del cormo de banano y alcanzar porcentajes de infección superiores al 80%, a 10 días de la inoculación en dosis de 1000 y 2000 JI.larva -1 . Los nematodos entomopatógenos cons - tituyen una alternativa viable a considerar dentro de los programas de manejo integrado del picudo negro, en cultivos como banano y plátano.

  5. Efecto del ácido indolacético sobre el desarrollo in vitro de los tejidos del fruto de naranjo dulce var. Salustiana

    Directory of Open Access Journals (Sweden)

    Alexander Rebolledo R.

    2007-01-01

    Full Text Available Se caracterizó el efecto de cuatro concentraciones (10- 8 a 10- 5 M de ácido indolacético (AIA y del tipo de explanto sobre el desarrollo in vitro de los tejidos del fruto del naranjo dulce Citrus sinensis (L Osbeck, de la variedad salustiana. La máxima respuesta se obtuvo con explantos de frutos de 6 días con las concentraciones más elevadas (10-5 y 10- 6 M. En este momento del desarrollo las células de los tejidos del fruto se encontraban en división. La capacidad de respuesta de los tejidos se redujo después de 31 días de desarrollo, cuando se inició la diferenciación celular del mesocarpo interno. El aumento en el tamaño de vesícula en explantos de frutos de 6 días de edad sugiere que el cultivar salustiana responde a la aplicación de auxinas durante el estado de desarrollo temprano del fruto, actuando directamente sobre este tejido.

  6. Establecimiento de un protocolo in vitro para el cultivo del ajo (Allium sativum en Costa Rica

    Directory of Open Access Journals (Sweden)

    Jaime Brenes-Madríz

    2014-11-01

    Full Text Available El cultivo del ajo en Costa Rica tiende a desaparecer, debido a la importación de esta hortaliza desde China y Taiwán y a la pérdida de calidad y vigor de la semilla, lo que ha provocado una reducción del área de siembra en el país. Para la investigación que se presenta en este artículo se utilizaron ajos cultivados en la zona de Llano Grande, Cartago, que pertenecen a pequeños agricultores que siembran con semilla heredada de generación en generación. Se evaluaron cuatro desinfecciones con hipoclorito de sodio a diferentes concentraciones y tiempos de exposición, además de cuatro medios de cultivo M&S (1962, con diferentes reguladores de crecimiento. La mayor sobrevivencia para el establecimiento in vitro se obtuvo lavando los bulbillos con agua y jabón y pasándolos después a una solución de Zetaran® y Agri-mycim® 5 g/l de cada uno por 45 minutos. Luego se realizó una segunda desinfección con hipoclorito de sodio al 3,5% p/v por 20 minutos en una cámara de flujo laminar y se realizaron tres lavados con agua bidestilada estéril. El medio de cultivo que presentó los mejores resultados fue el Murashige y Skoog (1962, complementado con 1mg/L de 2-isopenteniladenina (2ip y 2,5 mg/L de ácido indolacético (AIA. La tasa de sobrevivencia de los explantes oscila alrededor del 28,7% y se obtuvo un promedio de 1,6 brotes por explante, lo que se considera una tasa de brotación muy baja.

  7. Evaluación del efecto de un biopreparado de origen bacteriano en el cultivo in vitro del cafeto (coffea canephora p.)

    OpenAIRE

    González Vega, María Esther; Hernández Rodríguez, Annia; Ramos Navas, Ramón

    2011-01-01

    El presente estudio se realizó en el Instituto Nacional de Ciencias Agrícolas de Cuba, con el objetivo de evaluar el efecto de diferentes concentraciones del compuesto bacteriano CB-1 sobre el crecimiento y desarrollo in vitro de tres clones seleccionados de cafeto, var. Robusta, así como valorar la posible sustitución de la auxina AIA en el medio de cultivo establecido para la multiplicación del cafeto, donde se realizaron ensayos durante la fase de aclimatización de las vitroplantas. Se est...

  8. Some physicochemical characteristics of pinus (Pinus halepensis Mill., Pinus pinea L., Pinus pinaster and Pinus canariensis) seeds from North Algeria, their lipid profiles and volatile contents.

    Science.gov (United States)

    Kadri, Nabil; Khettal, Bachra; Aid, Yasmine; Kherfellah, Souraya; Sobhi, Widad; Barragan-Montero, Veronique

    2015-12-01

    Physicochemical characteristics of seeds of some pinus species (Pinus halepensis Mill., Pinus pinea L., Pinus pinaster and Pinus canariensis) grown in North Algeria were determined. The results showed that the seeds consist of 19.8-36.7% oil, 14.25-26.62% protein, 7.8-8.6% moisture. Phosphorus, potassium and magnesium were the predominant elements present in seeds. Pinus seed's oil physicochemical properties show acid values (4.9-68.9), iodine values (93.3-160.4) and saponification values (65.9-117.9). Oil analysis showed that the major unsaturated fatty acids for the four species were linoleic acid (30-59%) and oleic acid (17.4-34.6%), while the main saturated fatty acid was palmitic acid (5-29%). Gas Chromatography and Mass Spectrometry analysis of P. halepensis Mill., P. pinaster and P. canariensis volatile oils indicated that the major volatile compound was the limonene with relative percentage of 3.1, 7.5 and 10.8, respectively. Copyright © 2015 Elsevier Ltd. All rights reserved.

  9. Estudio del efecto inhibitorio de halo-tirosinas sobre la replicación in vitro del Virus Dengue

    Directory of Open Access Journals (Sweden)

    Sergio Yebrail Gómez Rangel

    2016-06-01

    Full Text Available Introducción: El dengue es una de las principales enfermedades virales transmitidas por vectores. La búsqueda de compuestos con potencial antiviral es una prioridad como estrategia para disminuir la morbilidad y mortalidad en la población, dada la ausencia de medicamentos y vacunas comercialmente disponibles. La obtención de múltiples fuentes de compuestos con potencial medicinal ha sido posible por estudios de bioprospección en diferentes zonas del país. Los autores previamente han demostrado el efecto inhibitorio sobre el Virus Dengue (DENV usando moléculas extraídas a partir de esponjas marinas (halotirosinas. Los resultados obtenidos a la fecha, sugieren la necesidad de continuar el estudio de este grupo de compuestos en la búsqueda de futuras alternativas terapéuticas para la enfermedad. Objetivo: Estudiar el posible efecto inhibitorio de halotirosinas sobre la replicación in vitro del DENV. Materiales y métodos: El estudio incluirá 14 halotirosinas obtenidas a partir de esponjas marinas. La citotoxicidad será estudiada en cultivo de células Vero (ATCC usando el método rutinario de MTT y será expresada como concentración citotóxica 50 (CC50 calculada por la evaluación de cada compuesto a diferentes concentraciones. La CC50 será usada como concentración de referencia para el estudio de inhibición de virus. El potencial antiviral será estimado por ensayo virucidal usando la cepa de referencia DENV-2/NG. Para esto, cantidades conocidas de virus serán incubadas con diferentes concentraciones de cada compuesto. La mezcla será adicionada a monocapas de células Vero a MOI de 1. La cantidad de virus al final será medida por ensayos de plaqueo y el porcentaje de inhibición será estimado como concentración efectiva 50 (CE50 al comparar cada uno con el control (virus sin compuesto. Los experimentos serán realizados por duplicado en 2 ensayos independientes. Para cada compuesto será informado un índice de selectividad

  10. Evaluación del residuo de cáscara de nuez (Juglans regia L.) en la producción de plántulas de Pinus patula, en vivero

    OpenAIRE

    Romero-Arenas, Omar; López Escobedo, Romelia; Damián Huato, Miguel Angel; Hernández Treviño, Israel; Parraguirre Lezama, José F.C.; Huerta Lara, Manuel

    2013-01-01

    Evaluation of residue of rind of nut(Juglans regia L.) in production of seedlings ofPinus patula, in pond. To produce quality plantsin the nursery, commercial substrates such asthe Peat Moss, perlite and vermiculite, are used;their cost is high and significantly reduces profitmargins; thus, there is a need to find alternativesubstrates. The shell of the walnut (Juglans regiaL.) is a residue without use in production; yetit has important nutrients that can be used byplant organisms. This study...

  11. Influencia del deterioro incipiente producido por el hongo de pudrición parda Serpula lacrymans, sobre las propiedades mecánicas de compresión normal y paralela a la fibra en madera de Pinus radiata D. Don

    Directory of Open Access Journals (Sweden)

    Pereira, A.

    2011-03-01

    Full Text Available Several buildings and structures of wood are attacked by rot fungi, leading in some cases, slight weight losses that result in reductions in the strength properties of the material. In this work was evaluated the influence of early rot decay in wood, in mechanical properties of normal and parallel compression. For this, were inoculated samples of Pinus radiata D. Don with brown rot fungi Serpula Lacrymans. These samples were tested in three differents periods of fungal exposure. The results identified a significant decrease in percentage of the properties under study.Diversas edificaciones y estructuras construidas en madera son atacadas por hongos de pudrición, provocando en algunos casos, leves pérdidas de peso que se traducen en disminución en las propiedades resistentes del material. En este trabajo se evaluó la influencia de estados incipientes de pudrición en madera, en las propiedades mecánicas de compresión paralela y compresión normal. Para esto, se inocularon probetas de Pinus radiata D. Don. con el hongo de pudrición parda Serpula lacrymans, las cuales fueron posteriormente ensayadas mecánicamente en tres diferentes períodos de exposición fúngica. Los resultados obtenidos determinaron una importante disminución porcentual de las propiedades bajo estudio.

  12. ASPECTOS INGENIERILES DEL CULTIVO IN VITRO DE CÉLULAS VEGETALES PARA LA PRODUCCIÓN DE METABOLITOS SECUNDARIOS

    OpenAIRE

    MARIO ARIAS; ANA AGUIRRE; MÓNICA ANGARITA; CAROLINA MONTOYA; JUAN RESTREPO

    2009-01-01

    Las plantas son una fuente importante de metabolitos que han sido usados como medicamentos, pesticidas, saborizantes y fragancias. Con el fin de producir estos compuestos sin necesidad de cultivar la planta completa, se ha propuesto la tecnología de cultivo de sus células in vitro (callos, suspensiones y órganos). En este trabajo se abordan diferentes aspectos ingenieriles del cultivo de células vegetales concernientes a la producción de metabolitos secundarios.

  13. Efecto del carbon activado y acido indol acetico en el desarrollo de protocormos de Masdevallia coccinea Linden ex Lindl. y Maxillaria nutans Lindl. in vitro

    National Research Council Canada - National Science Library

    Pedroza-Manrique, Jaime Alonso; Serrato-Munoz, Lina Constanza; Castano-Robayo, Mileidy

    2010-01-01

    .... En la evaluacion del desarrollo vegetativo bajo condiciones in vitro de los protocormos de Masdevallia coccinea y Maxillaria nutans, orquideas en vias de extincion, se obtuvo que solamente Maxillaria...

  14. Efecto antibacteriano del extracto etanólico del botoncillo (ACMELLA REPENS) sobre Porphyromona gingivalis: Estudio in Vitro

    OpenAIRE

    Andrea Lizbeth Chamorro Benalcázar; Marina Alejandra Cabrera Árias; Mariela Cumandá Balseca Ibarra

    2016-01-01

    Objetivo: Determinar el efecto antibacteriano del extracto etanólico de Botoncillo (Acmella repens) en diferentes concentraciones sobre la cepa de Porphyromona gingivalis. Materiales y metodos: En el presente estudio experimental, fueron utilizadas 24 cajas Petri con agar sangre, se inoculó P. gingivalis, y se colocaron discos con diferentes concentraciones del extracto etanólico de Botoncillo (25%, 50% y 100%), como sustancias control Clorhexidina al 0,12% y suero fisiológico. A los 7 días ...

  15. Regeneración natural de pino macho (Pinus caribaea Morelet var. caribaea Barret y Golfari para el incremento del índice de boscosidad en el macizo San Felipe-Los Joberos.

    Directory of Open Access Journals (Sweden)

    Pablo Antonio Cabrera Rodríguez

    2015-09-01

    Full Text Available Natural regeneration of pino macho (Pinus caribaea Morelet var. caribaea Barret and Golfari for increasing forest rate in the massive San Felipe-Los Joberos. In this paper the behavior of natural regeneration (RN of the male pine (Pinus caribaea Morelet var. caribaea Barret and Golfari is evaluated as an alternative reforestation of logged areas or affected by forest fires in the San Felipe-LosJoberos hills . For this, a study was conducted for four years (January 2009 to May 2013, in which the deposition of litter, number of seeds in the soil, number of trees / ha; height and mean diameter in the RN was determined, loading seed into the soil, and such as nearby trees isolated adult or adult plantations. Finally the costs incurred by way of planting and analyzed alternative management were evaluated. The results, therefore the number of plants as for its size, which makes it roughly equivalent to adjacent stands from plantations, indicate the effectiveness of this type of reforestation in the area. The economic advantages of natural regeneration on the plantation are shown, making it possible to develop strategies for management of the species in these conditions. Training decision-makers on environmental education is projected to mitigate the effect on forest cover or other environmental impacts while raising the culture for payment of services generated by forest vegetation.

  16. ASPECTOS INGENIERILES DEL CULTIVO IN VITRO DE CÉLULAS VEGETALES PARA LA PRODUCCIÓN DE METABOLITOS SECUNDARIOS

    Directory of Open Access Journals (Sweden)

    MARIO ARIAS

    2009-01-01

    Full Text Available Las plantas son una fuente importante de metabolitos que han sido usados como medicamentos, pesticidas, saborizantes y fragancias. Con el fin de producir estos compuestos sin necesidad de cultivar la planta completa, se ha propuesto la tecnología de cultivo de sus células in vitro (callos, suspensiones y órganos. En este trabajo se abordan diferentes aspectos ingenieriles del cultivo de células vegetales concernientes a la producción de metabolitos secundarios.

  17. Efecto antibacteriano del extracto etanólico del botoncillo (ACMELLA REPENS sobre Porphyromona gingivalis: Estudio in Vitro

    Directory of Open Access Journals (Sweden)

    Andrea Lizbeth Chamorro Benalcázar

    2016-09-01

    Full Text Available Objetivo: Determinar el efecto antibacteriano del extracto etanólico de Botoncillo (Acmella repens en diferentes concentraciones sobre la cepa de Porphyromona gingivalis. Materiales y metodos: En el presente estudio experimental, fueron utilizadas 24 cajas Petri con agar sangre, se inoculó P. gingivalis, y se colocaron discos con diferentes concentraciones del extracto etanólico de Botoncillo (25%, 50% y 100%, como sustancias control Clorhexidina al 0,12% y suero fisiológico. A los 7 días de incubación se midieron con una regla milimetrada los halos de inhibición formados alrededor de los respectivos discos. Resultados: el extracto de Botoncillo al 100% mostró diferencias significativas en comparación con la concentración del 25% y 50% (0 < 0.05. Al comparar el extracto de Botoncillo al 100% con la Clorhexidina 0,12% se observó valores de inhibición más altos para Clorhexidina 0,12%. Conclusión: El extracto etanólico de Botoncillo presentó un efecto antibacteriano sobre P. gingivalis.

  18. Relación entre edad del árbol y su composición química en Pinus radiata (D.Don) crecido en Chile y su importancia para la producción de bioetanol

    OpenAIRE

    Berrocal-Jiménez, Alexander; Instituto Tecnológico de Costa Rica, Escuela de Ingeniería Forestal; Cartago, Costa Rica; Freer-Calderón, Juanita; Universidad de Concepción, Chile; Baeza-Hernández, Jaime; Universidad de Concepción, Chile; Rodríguez-Gutiérres, Jaime; Universidad de Concepción, Chile; Espinosa-Bancalari, Miguel; Universidad de Concepción, Chile

    2012-01-01

    En la presente investigación se determinó la composición química de árboles de Pinus radiata (D.Don) de diferentes categorías de edad, para lo cual se utilizó un método de hidrólisis con ácido trifluoroacético (TFA). Se encontraron diferencias significativas en la composición química asociadas a la edad. Los valores obtenidos son necesarios para determinar la factibilidad de utilizar este recurso biomásico, a edades en que los contenidos de azúcares resultan tanto cuantitativamente, como cual...

  19. Estudio del efecto inhibitorio de halo-tirosinas sobre la replicación in vitro del Virus Dengue

    OpenAIRE

    Sergio Yebrail Gómez Rangel; Karen Sánchez; Andrea Lemus; Marcela Chaustre; Elkin Galeano; Marlen Martínez-Gutiérrez

    2016-01-01

    Introducción: El dengue es una de las principales enfermedades virales transmitidas por vectores. La búsqueda de compuestos con potencial antiviral es una prioridad como estrategia para disminuir la morbilidad y mortalidad en la población, dada la ausencia de medicamentos y vacunas comercialmente disponibles. La obtención de múltiples fuentes de compuestos con potencial medicinal ha sido posible por estudios de bioprospección en diferentes zonas del país. Los autores previamente han demostrad...

  20. Variación de las Características Anatómicas y de la Densidad Básica de la Madera en Árboles de Pinus Caribaea Morelet Varo Hondurensis Barret y Golfari en Función del Espaciamiento de Plantación

    Directory of Open Access Journals (Sweden)

    Vásquez Correal Angela María

    1998-06-01

    Full Text Available Los objetivos del presente trabajo fueron analizar las variaciones de la estructura anatómica, de los anillos de crecimiento y de la densidad básica de la madera de árboles de Pinus caribaea var, hondurensis, en un experimento de espaciamientos con 17 años de edad, instalado en la Estación Experimental de Recursos Naturales Renovables de Anhembi-SP, ESALQ-USP, Sao Paulo-Brasil. En este experimento fueron muestreados un total de 35 árboles, comprendidos en 3 clases de diámetro (dominantes, intermedios y dominados y en 3 diferentes tratamientos de espaciamiento 3, 01,5; 3, 02,5 Y 3, 03,5m. En cada uno de estos árboles se determino el diámetro a la altura del pecho (DAP y la altura. Después del apeo de los individuos se colectaron discos de madera en los árboles de la clase de diámetro caracterizada como intermedia, en los 3 espaciamientos (12 árboles en total y a la altura del DAP. De estas muestras se obtuvo el material necesario para la evaluación de las dimensiones de las traqueídas en los leños inicial y tardío, en el sentido médula-corteza. También se analizó en las muestras de madera de todos los árboles, en los tres espaciamientos, el espesor de los anillos de crecimiento y de los leños inicial y tardío y la densidad básica de la madera en 5 submuestras obtenidas en el sentido médula-corteza. Se establecieron, a partir de estas evaluaciones, las relaciones entre las características de la madera de Pinus caribaea var. hondurensis y sus modelos de variación, en función de las diferentes clases de diámetro de los árboles, en los diferentes espaciamientos. De acuerdo con los resultados la variación de las dimensiones de las traqueídas en el sentido médula-corteza en la madera de Pinus caribaea var. hondurensis presentó, con relación a su largo, en lodos los tratamientos de espaciamiento y para los dos tipos de leño, un aumento de casi el doble del 3 ° hasta el 7° anillo de crecimiento, con un

  1. Efecto del aglutinante y del desintegrante sobre la disolución de comprimidos de paracetamol : Estudios de bioequivalencia in vitro

    OpenAIRE

    Haile, Miriam M.; Pizzorno, María T.; Volonté, María Guillermina

    1992-01-01

    Se analizó el efecto de un aglutinante, polivinilpirrolidona (PVP), y de un desintegrante, almidón,sobre el proceso de disolución de comprimidos de paracetamol, calculándose una serie de parámetros cinéticos con el objeto de estimar la biodisponibilidad in vitro de los mismos. Los resultados muestran un aumento de la velocidad de disolución del paracetamol cuando el PVP es adicionado como solución a la formulación y una disminución cuando la concentración de almidón es menor. Se realizó un es...

  2. Cytoprotective effects of essential oil of Pinus halepensis L. against aspirin-induced toxicity in IEC-6 cells.

    Science.gov (United States)

    Bouzenna, Hafsia; Hfaiedh, Najla; Bouaziz, Mouhamed; Giroux-Metges, Marie-Agnès; Elfeki, Abdelfattah; Talarmin, Hélène

    2017-12-01

    Essential oils from Pinus species have been reported to have various therapeutic properties. This study was undertaken to identify the chemical composition and cytoprotective effects of the essential oil of Pinus halepensis L. against aspirin-induced damage in cells in vitro. The cytoprotection of the oil against toxicity of aspirin on the small intestine epithelial cells IEC-6 was tested. The obtained results have shown that 35 different compounds were identified. Aspirin induced a decrease in cell viability, and exhibited significant damage to their morphology and an increase in superoxide dismutase (SOD) and catalase (CAT) activities. However, the co-treatment of aspirin with the essential oil of Pinus induced a significant increase in cell viability and a decrease in SOD and CAT activities. Overall, these finding suggest that the essential oil of Pinus halepensis L. has potent cytoprotective effect against aspirin-induced toxicity in IEC-6 cells.

  3. Efecto antitumoral in vitro del aceite esencial de Piper aduncum L. (matico y su toxicidad oral en ratones

    Directory of Open Access Journals (Sweden)

    Jorge Arroyo

    2014-01-01

    Full Text Available Introducción: Piper aduncum (matico es una especie utilizada por sus propiedades medicinales en desórdenes gastrointestinales y genitourinarios. Objetivos: Evaluar el efecto antitumoral del aceite esencial de Piper aduncum (matico in vitro en siete líneas celulares tumorales humanas y determinar la toxicidad oral en ratones. Diseño: Experimental. Institución: Facultad de Medicina, Universidad Nacional Mayor de San Marcos, Lima, Perú. Material biológico: Líneas celulares tumorales humanas H460, DU-145, ME-180, K562, HT-29, MCF 7, M14, K562; fibroblastos normales de ratón 3T3 y ratones albinos machos Balb/C53. Intervenciones: Las líneas celulares fueron expuestas a cuatro concentraciones del aceite esencial de P. aduncum y 5-fluorouracilo (5-FU. Para la toxicidad oral se utilizó ratones albinos machos Balb C/53 de 40 días post destete, a cinco dosis de tratamiento, evaluándose el número de muertes en cada dosis. Principales medidas de los resultados: Porcentaje de inhibición del crecimiento celular (IC50, dosis letal 50 (DL50. Resultados: El aceite esencial mostró IC50 mayor a 250 ug/mL para las líneas celulares M-14 (r = -0,99; p 2 000 mg /kg. Conclusiones: El aceite esencial de P. aduncum no presentó efecto antitumoral in vitro para las siete líneas celulares tumorales humanas y no fue tóxico.

  4. Cultivo in vitro del tomate de árbol (Cyphomandra betacea (Cav. Sendt. (Fenotipo naranja proveniente de Costa Rica

    Directory of Open Access Journals (Sweden)

    Randall Chacón-Cerdas

    2014-06-01

    Se determinó que la desinfección con 2,5% de Ca(ClO2 fue la mejor para el establecimiento in vitro; el mejor medio de cultivo para la micropropagación del material fue el M1, compuesto por sales MS(1962 al 100%, sacarosa al 3%, phytagel® 1,8g/L, 0,5mg/L de AG3, 0,25mg/L de BAP y 2,0 mg/L de PaCa, el cual presentó el mejor balance entre el número promedio de brotación/explante y el número promedio de entrenudos/explante, sin formación excesiva de callo, mientras que el medio de cultivo E5, constituido por sales MS(1962 al 100%, agar 6,0g/L y sacarosa al 3%, sin reguladores del crecimiento y el medio de cultivo E6, que incluía las sales MS(1962 al 100%, agar 8,0g/L y sacarosa al 3%, sin reguladores del crecimiento, mostraron el periodo más corto para la formación de raíces, el mayor número promedio de raíces y la mayor longitud promedio de raíz y tallo.

  5. Estudio del efecto de la dirección de medición y contenido de humedad en la velocidad de ultrasonido en madera de Pinus radiata D. Don a través de análisis de varianza

    Directory of Open Access Journals (Sweden)

    Rodrigo Ortíz Mansilla

    2009-09-01

    Full Text Available Para analizar la técnica de ultrasonido como una alternativa en la evaluación del estado estructural de diferentes edificaciones construidas en madera, se estudió el efecto e importancia relativa de la dirección de medición y el contenido de humedad, so- bre la velocidad de transmisión en este material. Para esto, se aplicaron pulsos longitudinales ultrasónicos de 1 Mhz sobre pro- betas de madera de Pinus radiata D. Don, bajo un procedimiento experimental que consideró la utilización de un diseño fac- torial 22 completamente aleatorizado. Como resultado del análisis de varianza, se determinó que dentro de los rangos de con- tenido de humedad y dirección de medición estudiados, este último se presenta como factor significativo, explicando el 95% de la variabilidad total observada. De hecho, la variación del contenido de humedad entre 0 y 15%, conlleva a valores de veloci- dad de propagación promedio de 1.644 m/s y 2.130 m/s para las direcciones tangencial y radial, respectivamente. Los resulta- dos de este trabajo permiten concluir que al momento de realizar una evaluación mediante ultrasonido en estructuras en servi- cio, principalmente se debe considerar la dirección de medición como un factor determinante en el estudio; sin embargo, la e- fectividad de aplicación de esta técnica requiere de otros trabajos complementarios que consideren la propagación en planos mixtos de la madera. Este estudio se llevará a cabo próximamente.

  6. Evaluación del potencial antibacterial in vitro de Croton lechleri frente a aislamientos bacterianos de pacientes con úlceras cutáneas

    OpenAIRE

    Corrales Ramírez, Lucia; Castillo Castañeda, Adriana; Melo Vargas, Andrea

    2013-01-01

    Esta investigación tuvo como objetivo la evaluación del potencial antibacterial in vitro de Croton lechlerifrente a aislamientos bacterianos aeróbicos de pacientes con úlceras cutáneas del Sanatorio de Agua de Dios, Cundinamarca, Colombia. La metodología utilizada incluyó el aislamiento e identificación de los aislamientos bacterianos utilizando el sistema automatizado BBL-CrystalTM. Para la evaluación de la susceptibilidad antimicrobiana in vitro, se realizaron pruebas de difusión en dis...

  7. Efecto antibacteriano in vitro de diferentes concentraciones del extracto etan??lico de Caesalpinia spinosa (tara) frente a Streptococcus mutans ATCC 35668

    OpenAIRE

    AUTOR: Centuri??n Villar, Karina Mercedes

    2015-01-01

    El objetivo del presente estudio fue determinar el efecto antibacteriano in vitro del extracto etan??lico de Caesalpinia Spinosa (Tara) sobre Streptococcus mutans ATCC 35668. La muestra estuvo conformada por 64 observaciones, distribuidas en 4 grupos de 4 placas Petri cada uno, en cada placa se coloc?? el porcentaje de concentraci??n de estudio, frente a tres controles: control positivo (Clorhexidina al 0.12%), control negativo (Etanol) y un porcentaje de concentraci??n similar (para comp...

  8. Effect of two culture media on Pinus taeda shoots elongation

    Directory of Open Access Journals (Sweden)

    Luciana Paula Imbrogno

    2013-07-01

    Full Text Available Pinus taeda L. is a forest species of great international importance and in Argentina. Biotechnological techniques can provide an alternative to propagate this species, as well as for obtaining mother plants. The aim of this study was to achieve adequate elongation of in vitro shoots before transfer to the rooting stage. The shoots were obtained from acclimatized mother plants. It was disinfected for in vitro establishment. Two types of basal culture media: WV5 and DCR were studied. The best results were achieved with the combination of the WV5 salts supplement with 0.5% activated carbon, 0.01 mg l-1 ANA to obtain vigorous and longer than 40.0 mm in length shoots. Key words: forest, micropropagation, pine

  9. Estudios de simulaci??n del ecosistema ruminal en sistemas in vitro: aspectos metodol??gicos

    OpenAIRE

    Mart??nez Pel??ez, Mar??a Eugenia

    2011-01-01

    399 p. Los objetivos del presente trabajo de Tesis Doctoral fueron: 1) Analizar la evoluci??n temporal de los par??metros fermentativos y las comunidades de protozoos en fermentadores Rusitec. 2) Analizar los efectos de cambios en el ritmo de diluci??n y tiempos de retenci??n del concentrado sobre los par??metros fermentativos y la s??ntesis de prote??na microbiana en fermentadores Rusitec. 3) Comparar distintos m??todos habitualmente utilizados para el desligamiento d...

  10. Estudio in vitro para comprobar la estabilidad del color de materiales provisionales usados en prostodoncia

    OpenAIRE

    Blasi, Álvaro; Universitat Internacional de Catalunya, Barcelona, España; Barrero, Carlos H.; Universidad de Carolina del Norte, Chapel Hill, NC, EE. UU.

    2011-01-01

    Objetivo: en el presente estudio se valoró la estabilidad del color de resinas acrílicas y bisacrílicas. Métodos: se confeccionaron ochenta discos, la mitad fueron pulidos para comprobar cómo influye esto en la estabilidad del color. Las resinas se termociclaron y se sumergieron en diferentes líquidos pigmentantes. Se cuantificó el cambio en el color utilizando un espectrofotómetro. Resultados: los valores de cambio de color (DE) de todos los materiales fueron superiores al valor clínicamente...

  11. Actualidad y tendencias en el mercado del diagnóstico in vitro.

    OpenAIRE

    Gilberto Tillán Ochoa

    2005-01-01

    En el presente trabajo se presentan los diferentes sistemas y equipos actuales para el diagnóstico microbiológico in-vitro: VITEK-2 (BioMerieux), MICROSCAN WALKAWAY (Dade Behring), BIOMIC®Vision(Giles Scientific, Inc.), Bactometer® (bioMerieux), BacTrac® (Sy-Lab), Sensititre ARIS®, BD Phoenix¿ Automated Microbiology System (Becton-Dickinson), así como las nuevas tecnologías, métodos y técnicas utilizados en el diagnóstico in vitro: bioluminescencia de la Adenylate Kinase (AK), Point-Of-Care T...

  12. Evaluation of Trichoderma spp. and Clonostachys spp. Strains to Control Fusarium circinatum in Pinus radiata Seedlings Evaluación de Cepas de Trichoderma spp. y Clonostachys spp. para Controlar Fusarium circinatum en Plántulas de Pinus radiata

    Directory of Open Access Journals (Sweden)

    Priscila Moraga-Suazo

    2011-09-01

    Full Text Available The fungus Fusarium circinatum Nirenberg & O’Donnell causes pine pitch canker, an important disease for conifers worldwide. F. circinatum was first detected in Chile in 2001 and to date is present in nurseries and clonal hedges from Libertador General Bernardo O’Higgins Region to Los Rios Region. The purpose of this study was to evaluate the potential of Trichoderma spp. and Clonostachys spp. strains to control F. circinatum in Pinus radiata D. Don seedlings in the absence of other effective control methods. Eighty-one Trichoderma spp. and Clonostachys spp. strains were evaluated through in vitro assays to determine their ability to act as antagonists of F. circinatum and 21 strains were tested for their ability to reduce post-emergence mortality and increase P. radiata survival under greenhouse conditions. During in vitro experiments, 15 strains of Trichoderma inhibited mycelial growth of the pathogen by more than 60% and one strain of Clonostachys showed parasitism of F. circinatum hyphae. Greenhouse experiments showed no control of the disease when the antagonists were added to substrate after the pathogen. However, when the antagonists were added before the pathogen, four strains (Clonostachys UDC-32 and UDC-222 and Trichoderma UDC-23 and UDC-408 reduced post-emergence mortality between 80 and 100%. Among these strains, only Clonostachys UDC-222 significantly increased the survival of P. radiata seedlings. These results showed that Clonostachys UDC-222 has the potential to be used as a biocontrol agent against F. circinatum in the production of P. radiata plants.Fusarium circinatum Nirenberg & O’Donnell es el hongo que causa el cancro resinoso del pino, una enfermedad de importancia mundial en coníferas. En Chile, F. cicirnatum fue detectado por primera vez el año 2001 y a la fecha se encuentra presente en algunos viveros y huertos clonales desde la Región del Libertador General Bernardo O’Higgins hasta la Región de Los R

  13. Actividad citotóxica y leishmanicida in vitro del aceite esencial de manzanilla (Matricaria chamomilla)

    National Research Council Canada - National Science Library

    Yesmit Karina Ríos; Astrid Carolina Otero; Diana Lorena Muñóz; Mónica Echeverry; Sara María Robledo; Maira Alejandra Yepes

    2008-01-01

      Este estudio describe la evaluación de las actividades citotóxica y leishmanicida del aceite esencial de Matricaria chamomilla, una planta conocida como "manzanilla" a la que se le atribuye una variedad de usos en la medicina tradicional...

  14. Evaluación in vitro de la exactitud del localizador apical miniapex (sybronendo

    Directory of Open Access Journals (Sweden)

    Vanessa Bernal Alfaro

    2013-10-01

    Full Text Available Resumen El propósito de este estudio fue determinar el grado de exactitud del localizador Mini Ápex (Sybronendo, en la ubicación del CDC (constricción dentino cementaría cuando éste indica la medida de 0.0mm, confirmado con el microscopio de barrido electrónico. Se tomaron 84 conductos provenientes de las raíces mesiales de primeros molares inferiores permanentes, extraídos por motivos periodontales o protésicos, separadas de las raíces dístales, cortándolas a nivel de la unión amelocementaria, con un disco de carburo a baja velocidad, posteriormente se realizó el COE usando fresas gatees gliden # 2 y 3, irrigando entre cada una de ellas con NaOCl al 5.25% y se introdujeron limas k flex de sybronendo # 0.8, 10 o 15 dependiendo del diámetro del conducto. Se utilizó el método de Asako para determinar la longitud de trabajo, se fijaron las limas dentro del conducto con resina fluida, seguidamente se procedió a cortar el mango de las limas con un corta frió y se observó la posición del D0 de la lima con respecto al CDC con el SEM a una magnificación de 400 aumentos. Los resultados muestran que, en el 85.7%(72 conductos de las muestras, el D0 de la lima se encuentra entre 0.0-0.5mm del CDC, y el 14.3%(12 conductos se encuentran por debajo del CDC. La prueba de ANOVA no encontró diferencias estadísticamente significativas, demostrando la exactitud del localizador apical miniapex en la determinación de la longitud de trabajo del conducto radicular durante el tratamiento endodóntico. (DUAZARY 2011, 80 - 87AbstractThe purpose of this study was to determine the accuracy`s grade of the mini apex locator in the location of the CDC when this it indicates the measure of 0.0mm, confirmed with the SEM. 84 root canals were taken from permanent extracted lower first molars for periodontics of prostodontics reasons this were separated of the distal roots, cut them to level of the enamel-cementum joint, with a carbide disk to low speed

  15. Estructura química y actividad antioxidante in vitro del aceite esencial de Eryngium foetidum L. "siuca culantro"

    OpenAIRE

    Rodríguez Lichtenheldt, José Edwin

    2014-01-01

    El objetivo de este estudio fue elucidar la composición química del aceite esencial de las hojas frescas de Eryngium foetidum L. “siuca culantro” y su actividad antioxidante in vitro. El aceite esencial se obtuvo tratando 15 kg de hojas en un sistema de arrastre con vapor de agua, obteniéndose un rendimiento de 0,01% v/p con el que se realizó el análisis preliminar y fisicoquímico. Para el análisis de la composición química se utilizó el método cualitativo de Cromatografía de Gases / Espectró...

  16. COEXISTENCIA TRÓFICA DE AVES CARPINTERAS (Picidae EN UN BOSQUE DE Pinus cembroides (Zucc. DEL ÁREA NATURAL PROTEGIDA PEÑA ALTA, SAN DIEGO DE LA UNIÓN, GUANAJUATO

    Directory of Open Access Journals (Sweden)

    Saúl Ugalde-Lezama

    2011-01-01

    Full Text Available Estudiar la coexistencia trófica en aves es relevante para su manejo y conservación. Para ello, de enero a octubre de 2008 se identificaron técnicas de cacería y componentes entomológicos de las dietas de aves piciformes. Para la observación de las aves se usó la técnica de búsqueda intensiva en puntos de radio fijo, y para su captura, redes de niebla. Melanerpes formicivorous y Picoides scalaris prefirieron las técnicas de cacería Perforar y Colectar. Dichas técnicas se explicaronpor cinco y cuatro variables (P<0.05, respectivamente. Los estratos más utilizados fueron Pinus cembroides y Quercus spp. Los órdenes de insectos más consumidos fueron Coleóptera y Hemíptera. Las principales familias registradas corresponden a Scolytidae, Curculionidae, Pentatomidae, Cicadellidae, Cleridae y Melyridae. Los insectos más consumidos fueron los tipo Plaga (P, No Plaga (NP y Predadores (PR. Se registró una proporción significativa de individuos (X2=9.19, X2 0.001(5=20.5 de insectos en las dietas. Sin embargo, no fue posible registrar todos los fragmentos (X2=56.33, X2 0.001(5=20.5. El número de individuos (a=0.05, P-value=0.2317 y fragmentos (a=0.05, P-value=0.2606 entre especies fue similar. Siete variables (P<0.05 determinaron la ocurrencia de grasa y el consumo de presas. Los Picidos bajo estudio, a pesar de poseer anatomías, fisiologías y etologías similares, coexistieron tróficamente segregados durante la procuración alimenticia, minimizando de esta manera la competencia interespecífica.

  17. Plan Técnico de Manejo de los Bancos Clonales de Pinus pinea L. del Programa de Mejora Genética de la especie en España

    OpenAIRE

    Guadaño Peyrot, Carlos

    2014-01-01

    El presente Plan Técnico consiste en el diseño de los trabajos a realizar en los bancos clonales de pino piñonero gestionados por el Ministerio de Agricultura, Alimentación y Medio Ambiente [...] Grado en Ingeniería Forestal y del Medio Natural

  18. Efecto in vitro del D-002 sobre la actividad enzimática de la 5-lipoxigenasa (5-LOX

    Directory of Open Access Journals (Sweden)

    Yohani Pérez Guerra

    2012-06-01

    Full Text Available Introducción: el D-002, mezcla de seis alcoholes alifáticos primarios de alto peso molecular purificada de la cera de abejas, ha mostrado efectos antiinflamatorios, sin gastrotoxicidad secundaria, enmodelos experimentales. El tratamiento oral con D-002 reduce las concentraciones de leucotrieno B4 (LTB4 en exudados pleurales de ratas con pleuresía por carragenina, sugiriendo que podría inhibir la actividad enzimática de la 5-lipooxigenasa (5-LOX, si bien los mecanismos de la acción antiinflamatoria del D-002 no habían sido aún explorados. Objetivo: evaluar el efecto in vitro del D-002 sobre la actividad de la 5-LOX, utilizando la fracción citosólica de homogenatos de hígado de ratas. Métodos: las condiciones de ensayo utilizadas fueron las siguientes: fracción citosólica (50 µg de proteína disuelta en solución reguladora borato 0,2 mol/L (pH 9 y ácido linoleico (7,8-250 mmol/L como sustrato, ensayándose muestras paralelas incubadas con Tween-20/H2O (2 % (vehículo, muestras controles, D-002 (0,9-1 000 µg/mL o Lyprinol (500 µg/mL (sustancia de referencia. La actividad enzimática se evaluó mediante el cambio de absorbancia a 234 nm producido por la formación de dienos conjugados y medido en espectrofotómetro UV-visible. Resultados: la adición in vitro del D-002 produjo una inhibición significativa, dependiente de la dosis (r= 0,980; p< 0,001 (IC50= 95,34 µg/mL e incompetitiva de la actividad de la 5-LOX, cuya inhibición máxima (70 % se alcanzó con 500 µg/mL. Conclusiones: con este estudio se demuestra que el D-002 es capaz de inhibir la actividad enzimática de la 5-LOX, efecto que podría explicar, al menos parcialmente, su acción antiinflamatoria en modelos experimentales in vivo.

  19. Capacidad de humectación de soluciones irrigantes del tratamiento de conductos radiculares. In vitro

    OpenAIRE

    Anglas Machacuay, Abel; Universidad Nacional Mayor de San Marcos, Facultad de Odontología, Perú.; Pineda-Mejía, Martha; Universidad Nacional Mayor de San Marcos, Facultad de Odontología, Perú.; Salcedo-Moncada, Doris; Universidad Nacional Mayor de San Marcos, Facultad de Odontología.

    2017-01-01

    Objetivo: Determinar y analizar la capacidad de humectancia de soluciones irrigantes usadas en el tratamiento de conductos radiculares sobre la superficie dentinaria de dientes de bovino. Materiales y método: Estudio experimental in vitro. Se preparó 60 bloques de acrílicoautocurado de 40 mm x 30 mm x 8 mm donde una superficie expone tejido dentinario de diente de bovino. Los irrigantes de prueba fueron: solución de hipoclorito de sodio al 5,25%, gluconato de clorhexidina al 0.12%, suero fisi...

  20. Pinus halepensis, Pinus pinaster, Pinus pinea and Pinus sylvestris Essential Oils Chemotypes and Monoterpene Hydrocarbon Enantiomers, before and after Inoculation with the Pinewood Nematode Bursaphelenchus xylophilus.

    Science.gov (United States)

    Rodrigues, Ana M; Mendes, Marta D; Lima, Ana S; Barbosa, Pedro M; Ascensão, Lia; Barroso, José G; Pedro, Luis G; Mota, Manuel M; Figueiredo, A Cristina

    2017-01-01

    Pinewood nematode (PWN), Bursaphelenchus xylophilus, is the causal agent of pine wilt disease, a serious threat to global forest populations of conifers, especially Pinus spp. A time-course study of the essential oils (EOs) of 2-year-old Pinus halepensis, Pinus pinaster, Pinus pinea and Pinus sylvestris following inoculation with the PWN was performed. The constitutive and nematode inoculation induced EOs components were analyzed at both the wounding or inoculation areas and at the whole plant level. The enantiomeric ratio of optically active main EOs components was also evaluated. External symptoms of infection were observed only in P. pinaster and P. sylvestris 21 and 15 days after inoculation, respectively. The EO composition analysis of uninoculated and unwounded plants revealed the occurrence of chemotypes for P. pinaster, P. halepensis and P. sylvestris, whereas P. pinea showed a homogenous EO composition. When whole plants were evaluated for EO and monoterpene hydrocarbon enantiomeric chemical composition, no relevant qualitative and quantitative differences were found. Instead, EO analysis of inoculated and uninoculated wounded areas revealed an increase of sesquiterpenes and diterpenic compounds, especially in P. pinea and P. halepensis, comparatively to healthy whole plants EOs. © 2017 Wiley-VHCA AG, Zurich, Switzerland.

  1. Chemical composition, antimicrobial, insecticidal, phytotoxic and antioxidant activities of Mediterranean Pinus brutia and Pinus pinea resin essential oils.

    Science.gov (United States)

    Ulukanli, Zeynep; Karabörklü, Salih; Bozok, Fuat; Ates, Burhan; Erdogan, Selim; Cenet, Menderes; Karaaslan, Merve Göksin

    2014-12-01

    Essential oils of the resins of Pinus brutia and Pinus pinea were evaluated for their biological potential. Essential oils were characterized using GC-MS and GC/FID. in vitro antimicrobial, phytotoxic, antioxidant, and insecticidal activities were carried out using the direct contact and the fumigant assays, respectively. The chemical profile of the essential oils of the resins of P. pinea and P. brutia included mainly α-pinene (21.39% and 25.40%), β-pinene (9.68% and 9.69%), and caryophyllene (9.12% and 4.81%). The essential oils of P. pinea and P. brutia exerted notable antimicrobial activities on Micrococcus luteus and Bacillus subtilis, insecticidal activities on Ephestia kuehniella eggs, phytotoxic activities on Lactuca sativa, Lepidium sativum, and Portulaca oleracea, as well as antioxidant potential. Indications of the biological activities of the essential oils suggest their use in the formulation of ecofriendly and biocompatible pharmaceuticals. Copyright © 2014 China Pharmaceutical University. Published by Elsevier B.V. All rights reserved.

  2. 'Candidatus Phytoplasma pini', a novel taxon from Pinus silvestris and Pinus halepensis.

    Science.gov (United States)

    Schneider, Bernd; Torres, Ester; Martín, María P; Schröder, Manfred; Behnke, Heinz-Dietmar; Seemüller, Erich

    2005-01-01

    Pinus silvestris and Pinus halepensis trees grown in Germany and Spain, respectively, showing abnormal shoot branching, dwarfed needles and other symptoms were examined for the presence of plant-pathogenic mollicutes (phytoplasmas). While phytoplasmas could not be detected unambiguously with microscopical methods, PCR amplification using universal phytoplasma primers yielded positive results. Samples collected from symptomatic and non-symptomatic plant parts of both symptomatic Pinus silvestris and Pinus halepensis trees tested positive. Also, surrounding non-symptomatic trees proved to be phytoplasma-infected. Comparisons revealed that the 16S rRNA gene sequences of the phytoplasmas identified in Pinus silvestris and Pinus halepensis were nearly identical. However, the pine phytoplasma is only distantly related to other phytoplasmas. The closest relatives are members of the palm lethal yellowing and rice yellow dwarf groups and 'Candidatus Phytoplasma castaneae', which share between 94.5 and 96.6 % 16S rRNA gene sequence similarity. From these data it can be concluded that the phytoplasmas identified in the two Pinus species represent a coherent but discrete taxon; it is proposed that this taxon be distinguished at putative species level under the name 'Candidatus Phytoplasma pini'.

  3. Pinus taeda AND Pinus oocarpa PLYWOOD MANUFACTURING WITH FENOL-FORMALDHEYDE RESIN

    Directory of Open Access Journals (Sweden)

    Setsuo Iwakiri

    2002-01-01

    Full Text Available The objective of this study was to evaluate the quality of the plywood, manufactured from Pinus taeda and Pinus oocarpa, with 20 and 24 years old respectively, using three differentformulations of the fenol-formaldheyde resin. The results of the glue line shearing tests not showed statistical differences between the species and three resin formulations. In the hot water test, all of the boards were classified as “BR” and, the boards of the Pinus oocarpa produced with formulations (1and (3, were classified as “WBP”. The different resin formulations not influenced on the modulus ofelasticity (MOE and modulus of rupture (MOR The boards of Pinus taeda with formulations (1 and(2 showed higher values of MOE in comparison of the Pinus oocarpa. The MOR of the Pinus oocarpa withformulation (2 was higher than Pinus taeda. The mechanical properties of the plywood wereprobably influenced by wood variability related to sapwood and hartwood, thickness of growth ringsand, springwood and summerwood.

  4. EFECTO DEL CO-CULTIVO SOBRE EL DESARROLLO TEMPRANO DE EMBRIONES BOVINOS PRODUCIDOS IN VITRO

    Directory of Open Access Journals (Sweden)

    Angela López C.

    2007-12-01

    Full Text Available Objetivo. Estudiar el efecto del cocultivo con células oviductales sobre el porcentaje de clivaje 48 horas post inseminación (hpi de embriones bovinos en bajas tensiones de oxígeno. Materiales y métodos. Se recolectaron ovarios de matadero para la extracción de los ovocitos que fueron puestos en medio TCM 199 suplementado con hormonas, se fertilizaron con semen criópreservado y se cocultivaron en medio CR1aa con células de oviducto durante 48 horas. Se evaluó el porcentaje de clivaje total y el porcentaje de clivaje por estadios de 2-4 células y 5-8 células. La viabilidad de las células para el cocultivo se determinó por observación del movimiento ciliar y observación de monocapa. Los tratamientos fueron T1: células de oviducto + oxígeno 20%; T2: células de oviducto + oxígeno 7%; T3 y T4 fueron controles sin células para ambas concentraciones de oxígeno. Resultados. En cuanto al porcentaje de clivaje no hubo diferencia significativa entre los cuatro tratamientos, pero hubo una tendencia a mayor clivaje para los embriones cocultivados con células y 20% oxígeno. Conclusiones. La utilización de altas tasas de oxígeno (20% en los sistemas de cococultivo con células oviductales tienden a mejorar los porcentajes de clivaje a las 48 hpi.

  5. Uso de imágenes satélite Landsat para la detección de rodales de Pinus nigra Arn. y Pinus sylvestris L. afectados por escolítidos

    OpenAIRE

    Blanco, E.; Bonet Lledos, José Antonio; Eizaguirre Altuna, Matilde

    2009-01-01

    El uso de imágenes procedentes de sensores multiespectrales de resolución media como es el caso de Landsat TM ha sido ampliamente utilizado desde décadas para detectar, entre otras variables, el decaimiento y la defoliación provocada por plagas y enfermedades forestales. El presente trabajo evalúa la utilidad del uso de estas imágenes en la detección de rodales de pino laricio (Pinus nigra Arn.) y pino silvestre (Pinus sylvestris L.) afectados por escolítidos. El área de estudio se localizó e...

  6. Trypanocidal activity of oleoresin and terpenoids isolated from Pinus oocarpa.

    Science.gov (United States)

    Rubio, Julieta; Calderón, José S; Flores, Angélica; Castroa, Clementina; Céspedes, Carlos L

    2005-01-01

    Fractionation with n-hexane/ethyl acetate (1:1 v/v) by open column chromatography of the oleoresin from Pinus oocarpa Schiede yielded two diterpenes, pimaric acid (1) and dehydroabietic acid (5), the sesquiterpene longifolene (3) and a diterpenic mixture containing pimaric acid (1), isopimaric acid (4) and dehydroabietic acid (5). Subsequently, the isolated compounds, the mixture of 1, 4 and 5, the oleoresin and the dehydroabietic acid methyl ester (2), were tested in vitro against epimastigotes of Trypanosoma cruzi, the causative agent of Chagas disease. The most active compounds were 1, 3 and the oleoresin, being as active as nifurtimox, a drug effective in the treatment of acute infection by American trypanosomiasis and used in this work as positive control.

  7. Results from six Pinus taeda nursery trials with the herbicide ...

    African Journals Online (AJOL)

    Pendimethalin is used by some nursery managers to control weeds in Eucalyptus and Pinus seedbeds and cutting beds of Pinus. Six trials were implemented in open-rooted seedbeds to test the response of Pinus taeda to postemergence (to the crop) applications of 2.2 kg ha–1 active ingredient of pendimethalin (the ...

  8. Efecto de la criopreservación sobre la estructura del huso meiótico de ovocitos humanos madurados in vitro

    OpenAIRE

    Boiso Fedorovsky, Irene

    2001-01-01

    Consultable des del TDX Títol obtingut de la portada digitalitzada La presente tesis analiza el efecto de un protocolo de frecuente aplicación clínica (de congelación lenta y descongelación rápida, utilizando 1,2 propanediol y sacarosa como crioprotectores) sobre la supervivencia, la capacidad de maduración in vitro y la estructura del huso meiótico de ovocitos humanos, congelados en estadío de vesícula germinal y de metafase II. Para llevarlo a cabo se plantearon los siguientes objetiv...

  9. Efecto in vitro del D-003 sobre la actividad enzimática de la 5-lipoxigenasa (5-LOX

    Directory of Open Access Journals (Sweden)

    Yohani Pérez Guerra

    Full Text Available Introducción: el D-003, mezcla de ácidos alifáticos primarios superiores purificada de la cera de la caña, inhibe la síntesis de colesterol. Trabajos recientes han demostrado que el D-003 resulta efectivo en modelos experimentales de osteoartritis y que inhibe la actividad de las enzimas COX1 y COX2, preferentemente la COX1, sin producir gastrotoxicidad. Ha sido referido que los inhibidores duales de las enzimas COX y 5-LOX presentan efectos antinflamatorios desprovistos de gastrotoxicidad o que incluso, pueden resultar gastroprotectores. De acuerdo con estos antecedentes, el D-003 podría ser un inhibidor dual de dichas enzimas. Objetivo: investigar el efecto in vitro del D-003 sobre la actividad enzimática de la 5-LOX, utilizando la fracción citosólica de leucocitos polimorfonucleares de ratas. Métodos: se utilizaron las condiciones de ensayo siguientes: fracción citosólica (50 µg de proteína disuelta en solución reguladora borato 0,2 mol/L (pH 9 y ácido linoleico (7,8-250 mmol/L como sustrato, ensayándose muestras paralelas incubadas con Tween-20/H2O (2 % (vehículo, D-003 (0,6-6 000 µg/mL o extracto de Perna canaliculus (50 µg/mL (sustancia de referencia. Se evaluó la actividad enzimática mediante el cambio de absorbancia a 234 nm producido por la formación de dienos conjugados y medido en espectrofotómetro UV-visible. Resultados: la adición de D-003 produjo una inhibición dosis dependiente de la actividad enzimática de la 5-LOX (r= 0,975; p< 0,05 (CI50= 23,06 µg/mL in vitro. La magnitud de esta inhibición fue moderada, ya que la inhibición máxima, alcanzada a partir de 1 250 µg/mL, resultó de solo un 30 %. Conclusiones: el estudio demuestra que el D-003 es capaz de inhibir la actividad enzimática de la 5-LOX in vitro, pero moderadamente.

  10. Comportamiento in vitro del colágeno de la unión amelodentinaria en premolares humanos sometidos a altas temperaturas

    Directory of Open Access Journals (Sweden)

    Sebastián Medina

    2015-12-01

    Full Text Available Objetivo: Determinar el comportamiento in vitro del colágeno de la unión amelo-dentinaria en premolares humanos sometidos a altas temperaturas. Materiales y métodos: Estudio descriptivo pseudo-experimental in vitro de corte transversal que determinó, a través de microscopía electrónica de barrido, el comportamiento físico del colágeno de la unión amelodentinaria en 60 premolares humanos sometidos a altas temperaturas (200°C, 400°C, 600°C, 800°C y 1.000°C, para proporcionar evidencia científica que permita emplear la separación del esmalte y la dentina como un marcador fehaciente repetitivo de utilidad en los procesos de identificación odontológica y documentación de la necropsia médico-legal para el caso de cadáveres o restos humanos que resulten quemados, carbonizados o incinerados. Resultados: Se observa que el diseño micro-estructural del patrón reticular de colágeno de la dentina en relación con los cristales de hidroxiapatita resulto afectado conforme aumentaba la temperatura. Conclusiones: En conjunto, la alteración del patrón reticular del colágeno y los cambios micro-estructurales de la hidroxiapatita de calcio (fusión y sinterización de las nano-esferas de fosfato octa-cálcico de la dentina y del esmalte, explican la separación gradual y progresiva de estos dos tejidos a nivel de la unión amelo-dentinaria.

  11. Silvical characteristics of pitch pine (Pinus rigida)

    Science.gov (United States)

    S. Little

    1959-01-01

    Pitch pine (Pinus rigida Mill.) grows over a wide geographical range - from central Maine to New York and extreme southeastern Ontario, south to Virginia and southern Ohio, and in the mountains to eastern Tennessee, northern Georgia, and western South Carolina. Because it grows mostly on the poorer soils, its distribution is spotty.

  12. Ecosystem carbon stocks in Pinus palustris forests

    Science.gov (United States)

    Lisa Samuelson; Tom Stokes; John R. Butnor; Kurt H. Johnsen; Carlos A. Gonzalez-Benecke; Pete Anderson; Jason Jackson; Lorenzo Ferrari; Tim A. Martin; Wendell P. Cropper

    2014-01-01

    Longleaf pine (Pinus palustris Mill.) restoration in the southeastern United States offers opportunities for carbon (C) sequestration. Ecosystem C stocks are not well understood in longleaf pine forests, which are typically of low density and maintained by prescribed fire. The objectives of this research were to develop allometric equations for...

  13. Biodeteriorasi kayu pinus (Pinus merkusii oleh rayap tanah Macrotermes gilvus Hagen (Blattodea: Termitidae

    Directory of Open Access Journals (Sweden)

    NIKEN SUBEKTI

    2012-11-01

    Full Text Available Subekti N. 2012. Biodeteriorasi kayu pinus (Pinus merkusii oleh rayap tanah Macrotermes gilvus Hagen (Blattodea: Termitidae. Bioteknologi 9: 57-65. Rayap tanah Macrotermes gilvus Hagen memiliki peran penting dalam proses disintegrasi dan dekomposisi material organik dari kayu dan serasah tanaman. Penelitian ini bertujuan untuk mengkaji biodeteriorasi kayu pinus (Pinus mercusii oleh rayap tanah M. gilvus dan analisis komponen kimia kayu. Untuk menguji tingkat konsumsi makan rayap M. gilvus digunakan metode Surnuwat (1996. Untuk mengetahui perubahan kayu pinus dilakukan analisis komponen kimia dan Scanning Electron Microscope (SEM. Hasil penelitian menunjukkan bahwa konsumsi makan tunggal dan konsumsi makan ganda termasuk rentan terhadap rayap, tingkat konsumsi makan rayap M. gilvus terhadap kayu pinus rata-rata 0, 82 mg/hari selama lima hari pengamatan. Sementara itu, rata-rata penurunan berat kayu pada uji preferensi ganda dalam koloni adalah 57.95% selama tiga bulan. Komposisi kimia kayu adalah 21,91% lignin, 60,92% holoselulosa dan 43,64 sellulosa. Penurunan kekerasan pada kayu, perubahan sifat dan komposisi kimia kayu menyebabkan kerusakan pada dinding sel kayu dan diduga meningkatkan preferensi makan rayap M. gilvus.

  14. Evaluación de la eficacia in vitro e in vivo del tratamiento antimicrobiano combinado por el tablero de damas Effectiveness of a combined antimicrobial treatment by means of the draughtboard. In vitro and in vivo assessment

    Directory of Open Access Journals (Sweden)

    María Espino Hernández

    2009-08-01

    Full Text Available El presente trabajo tuvo como objetivo, evaluar la eficacia clínica del tratamiento antimicrobiano combinado mediante pruebas de sinergismo in vitro y determinar la eficacia del procedimiento empleado para orientar la aplicación de estas formas de tratamiento en el paciente con sepsis por bacterias con resistencia variada. Se evaluó el tratamiento aplicado a 163 recién nacidos con sepsis corroborada por hemocultivo positivo en el Hospital Ginecobstétrico Universitario "América Arias", durante el periodo de enero de 1993 a diciembre de 2000, por el método tablero de damas (checkerboard en placas de microtitulación. Las combinaciones de fármacos sinérgicas in vitro presentaron una alta probabilidad de eficacia clínica, independiente del patrón de susceptibilidad del microorganismo aun frente a cepas resistentes a los 2 antimicrobianos utilizados en el tratamiento. Todos los resultados antagónicos in vitro se correspondieron con un fallo clínico terapéutico. El procedimiento utilizado puede constituir una valiosa herramienta para orientar la terapéutica en pacientes con sepsis por microorganismos resistentes.The aim of present paper was to evaluate clinical effectiveness of combined antimicrobial treatment by means of in vitro synergism tests and to determine effectiveness of used procedure to direct applications of these treatment strategies in patient presenting with sepsis from varied resistance bacteria. Treatment applied was assessed in 163 newborn patients presenting with sepsis from positive hemoculture in "America Arias" University Gynecology-Obstetrics Hospital from January 1993 to December 2000, by checkerboard method in microtritate plates. In vitro synergic drug combinations showed a high probability of clinical effectiveness, independently of susceptibility pattern of microorganism event versus resistant strains to both antimicrobials used in treatment. All in vitro antagonistic results correspond both with a

  15. Post-fire ecological restoration of a mixed Pinus-Quercus forest in northeastern Mexico

    Directory of Open Access Journals (Sweden)

    Eduardo Alanís-Rodríguez

    2015-01-01

    Full Text Available La presente investigación se desarrolló en un bosque de Pinus-Quercus en el Parque Ecológico Chipinque (noreste de México, el cual fue afectado por un incendio forestal y sometido a tratamiento de restauración ecológica. Los objetivos fueron evaluar el establecimiento artificial de Pinus pseudostrobus (Lindl. y analizar el efecto de las barreras de retención de suelo después de 10 años de su instalación. Para ello se estableció un área de estudio con tratamiento de restauración ecológica y otra área sin tratamiento en las que se muestreó la comunidad vegetal y la profundidad del suelo. De acuerdo con los resultados, se registró 35 % de supervivencia de la plantación, la cual se considera como no aceptable. Las barreras de retención de suelo tuvieron efecto positivo, pues incrementaron la profundidad del suelo hasta 25 %. Con la investigación se concluye que las técnicas de restauración post-incendio aplicadas han sido eficaces, ya que incorporan una especie clave de ecosistemas maduros y evitan la pérdida de suelo por arrastre, por lo que se recomienda su uso en rodales de Pinus-Quercus afectados por incendios en la Sierra Madre Oriental.

  16. CARACTERIZACIÓN Y EVALUACIÓN in vivo E in vitro DEL LIPOPOLISACÁRIDO DE Aeromonas hydrophila

    Directory of Open Access Journals (Sweden)

    AP JIMÉNEZ

    2008-01-01

    Full Text Available A partir de una cepa de A. hydrophila aislada de un brote de enfermedad septicémica en Tilapia nilótica (Piaractus brachypomusoreochromis niloticus, se obtuvieron extractos de lipopolisacárido (LPS crudo (29,5 mg/ml y semipurificado (106,5 mg/ml mediante la técnica fenol-agua caliente descrita por Westphal, Jann (1965. La presencia de proteína fue del 2,3% para el extracto crudo y de 0,1% para el semipurificado; la concentración de polisacáridos osciló entre el 15 y 26%. En electroforesis (SDS-PAGE se observaron bandas de 14 Kd correspondientes al oligosacárido central y al lípido A del LPS. Tres ratones de 25-35 g fueron inoculados intraperitonealmente con 25 mg/Kg de LPS cru- do, a partir de la primera hora todos los animales mostraron erizamiento, taquipnea e inapetencia; microscópicamente se detectó congestión hepática y pulmonar, hemorra- gias pulmonares y renales, marginación leucocitaria en hígado y pulmón con predomi- nio de polimorfonucleares neutrófilos (PMN en todos los animales, mostrando un mayor efecto que el control inoculado con LPS de E. coli (Sigma® a la misma concen- tración. In vitro el LPS crudo a concentración de 10, 20 y 30 μg/ml indujo proliferación de células mononucleares murinas (2,0 x 105 en 200 μl de medio DMEM por incorpo- ración de timidina tritiada; tanto el LPS control (E. coli, como el LPS crudo de A. hydrophila mostraron cuentas por minuto (CPM ascendentes de manera dosis depen- diente, el LPS de A. hydrophila desencadeno una proliferación muy similar a la inducida por el control.

  17. In vitro regeneration of Pinus brutia Ten. var. eldarica (Medw ...

    African Journals Online (AJOL)

    2Institute of Biotechnology and Applied Ecology, Universidad Veracruzana, Campus for Culture, Arts and Sport,. Xalapa, Veracruz. C.P. 91001, Mexico. 3Laboratory of Plant Biotechnology and .... abscisic acid (ABA), as well as the absence of growth promoters, such as GA3. The loss of embryo dormancy is often associated ...

  18. Dendroclimatic analysis of Pinus pseudostrobus and Pinus devoniana in the municipalities of Áporo and Zitácuaro (Michoacán, Monarch Butterfly Biosphere Reserve

    Directory of Open Access Journals (Sweden)

    Jaume Marlès Magre

    2015-12-01

    Full Text Available This article presents the first study on dendroclimatology of Pinus pseudostrobus and Pinus devoniana in the state of Michoacán (Mexico, specifically in the municipalities of Áporo and Zitácuaro, both municipalities within the Monarch Butterfly Biosphere Reserve (MBBR. The sampling in Áporo, northwest of the MBBR, was held in Los Ejidos del Rincón del Soto and Arroyo Seco, in Sierra Chincúa (May 2011. In Zitácuaro, southwest of the reserve, a sampling was performed in the Ejido de San Juan de Zitácuaro, in the area of Ocotal and Palma, and Meso Sedano (June 2011. There were a total of 38 Pinus pseudostrobus and 12 Pinus devoniana sampled in both areas of the study and distributed in 28 trees in the municipality of Áporo and 22 in Zitácuaro. Two samples per tree were taken at 1.3 m height, resulting in a total of 100 tree cores. The dendrochronological series in Áporo for the species Pinus pseudostrobus were extended to 62 years (1949-2010 and for Pinus devoniana 86 years (1925-2010; and the series in Zitácuaro for Pinus pseudostrobus and Pinus devoniana were extended to 47 years (1964-2010 and 44 years (1967-2010, respectively. The ring chronologies were validated using the program COFECHA, which calculates the cross correlations between individual series of the tree-growth, five series were eliminated due to very low or negative correlations. The climate data from Zitácuaro were obtained from two weather stations located in the same municipality. And, in the case of Áporo, the data was obtained from stations located in Senguio. The growth rates related to the climate were obtained by removing the growth trend of each tree due to the age, size and other factors such as the competition, using the program ARSTAN. The following statistics were used to evaluate the quality of the residual chronologies and to determine the potential dendrochronology of species for the different populations: the average correlation between series (Rbar

  19. Biocontrol of Fusarium circinatum Infection of Young Pinus radiata Trees

    Directory of Open Access Journals (Sweden)

    Eugenia Iturritxa

    2017-01-01

    Full Text Available Pitch canker, caused by the fungus Fusarium circinatum, is a major disease of Pinus radiata currently controlled to some extent in nurseries by good hygiene and application of synthetic fungicides. The aim of this study was to evaluate alternative strategies to control fungal infections in nurseries and young pine plantations. The antagonistic effects of biocontrol bacteria and essential oils against F. circinatum in vitro and in young P. radiata trees were assessed. Pseudomonas fluorescens, Erwinia billingiae, and Bacillus simplex reduced the growth of the fungus in vitro by 17%–29%, and decreased the density of the mycelial mat. In young P. radiata trees, the length of F. circinatum lesions was reduced by 22%–25% by the same bacterial strains. Direct application of cinnamon and/or clove essential oils to wounds in stems of two-year-old P. radiata trees also limited the damage caused by F. circinatum. Lesion length was reduced by 51% following treatment with cinnamon oil (10% v/v, and by 45% following treatment with clove oil (15% v/v or a combination of both oils. However, the oils were toxic to younger trees. The biocontrol bacteria and essential oils show promise as prophylactic treatments to reduce the devastating effects of F. circinatum on P. radiata.

  20. Comparative mapping in Pinus: sugar pine (Pinus lambertiana Dougl.) and loblolly pine (Pinus taeda L.).Tree Genet Genomes 7:457-468

    Science.gov (United States)

    Kathleen D. Jermstad; Andrew J. Eckert; Jill L. Wegrzyn; Annette Delfino-Mix; Dean A Davis; Deems C. Burton; David B. Neale

    2011-01-01

    The majority of genomic research in conifers has been conducted in the Pinus subgenus Pinus mostly due to the high economic importance of the species within this taxon. Genetic maps have been constructed for several of these pines and comparative mapping analyses have consistently revealed notable synteny. In contrast,...

  1. Inventario forestal del monte M-2009 del elenco denominado "Plaza de Buenavista" perteneciente al término municipal de Zarzalejo (Madrid)

    OpenAIRE

    Rica Fuentes, Amparo

    2012-01-01

    Se hace un estudio del monte de origen repoblación. Su especie principal es Pinus pinaster Ait., la secundaria es Pinus sylvestris L. y tiene presencia de Pinus nigra Arn. que no estaba prevista en el proyecto de repoblación, pero probablemente apareciera por mezcla de semillas. En los años 40 se cambió el uso del monte de agrícola a forestal, claro indicio de la baja productividad del suelo para el cultivo de cereales; de esta forma se dio a los propietarios de las distintas parcelas l...

  2. Hydraulic adjustments underlying drought resistance of Pinus halepensis

    National Research Council Canada - National Science Library

    Klein, Tamir; Cohen, Shabtai; Yakir, Dan; Tognetti, Roberto

    2011-01-01

    .... Our objective was to investigate under controlled conditions the hydraulic adjustments underlying the observed ability of Pinus halepensis to survive seasonal drought under semi-arid conditions...

  3. Efecto del ácido indolacético sobre el desarrollo in vitro de los tejidos del fruto de naranjo dulce var. Salustiana Effect of the indolacetic acid on the in vitro development of the fruit tissues of the sweet orange var. Salustiana

    Directory of Open Access Journals (Sweden)

    Alexander Rebolledo R.

    2007-01-01

    Full Text Available Se caracterizó el efecto de cuatro concentraciones (10- 8 a 10- 5 M de ácido indolacético (AIA y del tipo de explanto sobre el desarrollo in vitro de los tejidos del fruto del naranjo dulce Citrus sinensis (L Osbeck, de la variedad salustiana. La máxima respuesta se obtuvo con explantos de frutos de 6 días con las concentraciones más elevadas (10-5 y 10- 6 M. En este momento del desarrollo las células de los tejidos del fruto se encontraban en división. La capacidad de respuesta de los tejidos se redujo después de 31 días de desarrollo, cuando se inició la diferenciación celular del mesocarpo interno. El aumento en el tamaño de vesícula en explantos de frutos de 6 días de edad sugiere que el cultivar salustiana responde a la aplicación de auxinas durante el estado de desarrollo temprano del fruto, actuando directamente sobre este tejido.The effect of 4 concentrations of the indolacetic acid as well as the explant type on the in vitro development of the sweet orange fruit tissue Citrus sinensis (L Osbeck Salustiana variety was characterized. A maximum response in explants of 6-d-old fruitlets was obtained with the higher concentrations (10-5 and 10- 6 M. At this development stage, fruit tissue cells were dividing. A decrease in the fruit response capacity after day 31 was verified. At this moment, cellular differentiation in the internal mesocarp was starting. The increase in size of the vesicle explants of 6-d-old fruitlets suggests a response of the Salustiana cultivar to auxin applications during the early stage of fruit development. This hormone can operate directly on this tissue.

  4. Efecto de la progesterona y del acetato de medroxiprogesterona (MPA) sobre el crecimiento de fibrosarcomas murinos : Estudios in vivo e in vitro

    OpenAIRE

    Lanari, Claudia Lee Malvina

    1985-01-01

    El objetivo de este trabajo surgió de la observación de que la progesterona tiene efectos antifibromatogénicos en cobayos y en el hombre. Se evaluó el efecto de la progesterona y del acetato de medroxiprogesterona (MPA) sobre el crecimiento de un fibrosarcoma murino tanto in vivo como in vitro. También se estudió su efecto sobre la inducción de sarcomas originados por la implantación subcutánea de un cuerpo extraño. (cilindro de vidrio). In vivo, los resultados obtenidos demuestran que el MPA...

  5. EFECTO ANTIBACTERIANO in vitro DEL EXTRACTO ALCOHÓLICO DE Psidium guajava (guayaba) y Medicago sativa (alfalfa) SOBRE Streptococcus mutans ATCC 25175

    OpenAIRE

    Chero Nepo, Diego Armando; Universidad Señor de Sipán; Ruiz Barrueto, Miguel Ángel; Universidad Señor de Sipán

    2016-01-01

    Objetivo: Determinar el efecto de antibacteriano in vitro del extracto alcohólico de Psidium guajava y Medicago sativa sobre Streptococcus mutans ATCC 25175. Material y métodos: Investigación cuantitativa con diseño experimental de estímulo creciente. Se emplearon 20 concentraciones volumétricas de cada extracto de 1 - 20 mg/ml, un control positivo que fue clorhexidina al 0,12% y el control negativo que fue etanol absoluto. Para evaluar el efecto antibacteriano de ambos extractos alcohólicos ...

  6. Evaluación de la fertilidad in vitro del semen de toros jóvenes nacionales en ovocitos provenientes de ovarios de animales beneficiados

    OpenAIRE

    Cabrera V., Prospero; Universidad Nacional Agraria La Molina (Perú).; Yoong K., Washington; Universidad Nacional Agraria La Molina (Perú).

    2016-01-01

    El presente trabajo se desarrolló en el Centro de Investigación y Enseñanza en Transferencia de Embriones (CIETE), convenio Universidad Nacional Agraria La Molina (UNALM) - Ministerio de Agricultura (MINAG); ubicada en el campus de la UNALM. El objetivo fue evaluar la fertilidad y la producción de embriones in vitro de cuatro toros provenientes del Banco Nacional de Semen: Pepe, Miguel, Juanjo y Vito. Para este estudio se utilizaron 1031 ovocitos de calidad A y B aspirados de ovarios de vacas...

  7. The flexural properties of young Pinus elliottii × Pinus caribaea var ...

    African Journals Online (AJOL)

    The objectives of this study were to determine the bending strength and stiffness properties of young Pinus elliottii × P. caribaea var. hondurensis timber from the Southern Cape, South Africa, and to evaluate the predictability of these properties from acoustic measurements on standing trees, logs and their sawn boards.

  8. Needle Terpenes as Chemotaxonomic Markers in Pinus: Subsections Pinus and Pinaster.

    Science.gov (United States)

    Mitić, Zorica S; Jovanović, Snežana Č; Zlatković, Bojan K; Nikolić, Biljana M; Stojanović, Gordana S; Marin, Petar D

    2017-05-01

    Chemical compositions of needle essential oils of 27 taxa from the section Pinus, including 20 and 7 taxa of the subsections Pinus and Pinaster, respectively, were compared in order to determine chemotaxonomic significance of terpenes at infrageneric level. According to analysis of variance, six out of 31 studied terpene characters were characterized by a high level of significance, indicating statistically significant difference between the examined subsections. Agglomerative hierarchical cluster analysis has shown separation of eight groups, where representatives of subsect. Pinaster were distributed within the first seven groups on the dendrogram together with P. nigra subsp. laricio and P. merkusii from the subsect. Pinus. On the other hand, the eighth group included the majority of the members of subsect. Pinus. Our findings, based on terpene characters, complement those obtained from morphological, biochemical, and molecular parameters studied over the past two decades. In addition, results presented in this article confirmed that terpenes are good markers at infrageneric level. © 2017 Wiley-VHCA AG, Zurich, Switzerland.

  9. Simulador de crecimiento para el manejo de rodales coetáneos de Pinus patula.

    OpenAIRE

    Santiago García, Wenceslao

    2013-01-01

    El manejo forestal en la región de Zacualtipán, Hidalgo, México, se ha enfocado en las últimas tres décadas hacia rodales coetáneos de Pinus patula, la especie maderable nativa de rápido crecimiento y alto valor comercial. El manejo adecuado de P. patula requiere de herramientas cuantitativas confiables que permitan predecir los niveles de crecimiento y rendimiento maderable, controlar la densidad del rodal y programar los aclareos, estimar las clases de productos a obtener, y optimizar el tu...

  10. Effect of years and origins into the seed production of Scots Pine (Pinus sylvestris) in Finland

    OpenAIRE

    Díez Alonso, María

    2013-01-01

    Ocho áreas ocupadas de forma natural por Pinus sylvestris y localizadas a diferentes latitudes en Finlandia fueron seleccionadas y estudiadas desde 1997 hasta 2001para analizar las diferencias en el ratio de flores femeninas que desarrollan cono y el número de semillas por cono. Las condiciones climatológicas, las condiciones del lugar y el tiempo de floración son algunos de los factores que pueden afectar la producción de semilla. Los resultados muestran la posibilidad de existen...

  11. An evaluation of wood properties of Pinus caribeae (Morelet) in ...

    African Journals Online (AJOL)

    This paper examines the effects of within tree variation on wood density, ring width and anisotropic shrinkage of Pinus caribeae (Morelet) among tree partitions in Oluwa pine plantation. Five 15-year old Pinus caribeae (Morelet) in three partitions were randomly selected from the plantation and felled for the study. Wood ...

  12. Effect on nursery and field performance of Pinus patula seedlings ...

    African Journals Online (AJOL)

    Fusarium circinatum is an important fungal pathogen of Pinus species. In South Africa, it is the most significant pathogen of Pinus patula seedlings in forestry nurseries where it presents a substantial constraint to productivity and can continue to cause mortality in-field for up to two years after establishment. This study ...

  13. Germination and early seedling growth of Pinus densata Mast. provenances

    Science.gov (United States)

    Yulan Xu; Nianhui Cai; Bin He; Ruili Zhang; Wei Zhao; Jianfeng Mao; Anan Duan; Yue Li; Keith Woeste

    2016-01-01

    We studied seed germination and early seedling growth of Pinus densata to explore the range of variability within the species and to inform afforestation practices. Phenotypes were evaluated at a forest tree nursery under conditions that support Pinus yunnanensis, one of the presumed parental species of P. densata...

  14. Estudio in vitro del espesor radicular residual dejado por diferentes sistemas rotatorios en la zona de peligro

    OpenAIRE

    Luna Lara, Carlos Alberto

    2014-01-01

    INTRODUCCIÓN: La adecuada limpieza y conformación del conducto radicular consiste en darle una forma al conducto que permita remover el tejido pulpar vital o necrótico, dentina y microorganismos y facilitar la obturación tridimensional de esté. Uno de los principales retos de la endodoncia es la preparación de los conductos curvos ya que se pueden producir accidentes de procedimiento como: la transportación del conducto, fractura de instrumentos, adelgazamiento excesivo de las paredes del con...

  15. Características de calidad y digestibilidad in vitro del almidón agrio de yuca (Manihot esculenta producido en Costa Rica

    Directory of Open Access Journals (Sweden)

    Pedro Vargas Aguilar

    2012-06-01

    Full Text Available Se evaluaron las características físicas, químicas y microbiológicas del almidón agrio de yuca producido a partir de las variedades de yuca ‘Valencia’ y ‘Brasileña’. El almidón agrio se obtuvo luego de un proceso de fermentación natural por 30 días y una posterior deshidratación mediante dos métodos: exposición directa al sol y en un secador solar. El proceso de fermentación para los almidones de ambas variedades se caracterizó por un aumento de la acidez titulable. Las propiedades físicas y química del almidón agrio de las dos variedades secados bajo los dos métodos se compararon con el almidón nativo. Se obtuvo una capacidad de expansión mayor (p 0,05. Se observó una forma esférica y truncada, así como la presencia del hilum en los gránulos de todos los almidones estudiados, sin embargo, en los almidones agrios presentó una apariencia con perforaciones, hendiduras y fisuras. Con respecto al tamaño de los gránulos se obtuvo un intervalo de 12,8 - 14,0 µm para el almidón nativo, mientras que el almidón agrio presentó valores de 11,3 - 11,6 µm. Se midió la digestibilidad in vitro del producto horneado obtenido de la prueba de expansión del almidón agrio variedad ‘Valencia’ y deshidratado en secador solar, por considerarse el más factible de procesar industrialmente. Se obtuvo 44 g/100 g de almidón lentamente digerible.

  16. Asexual propagation of Pinus leiophylla Schiede ex Schltdl. et Cham.

    Directory of Open Access Journals (Sweden)

    Juan Carlos Cuevas-Cruz

    2015-01-01

    Full Text Available Resumen El efecto del sustrato, tipo de estaca y concentración de ácido indolbutírico (AIB se evaluó en el enraizado de estacas de Pinus leiophylla con el propósito de multiplicar progenies de familias de medios hermanos con crecimiento sobresaliente. Para ello se utilizaron dos tipos de sustratos (agrolita y una mezcla de turba-agrolita-vermiculita 1:1:1, dos tipos de estacas (apicales y basales y dos concentraciones de AIB (0 y 10,000 ppm. La probabilidad y porcentaje de enraizado, crecimiento de la estaca y características de las raíces formadas de P. leiophylla se evaluaron. Los resultados indican que usando estacas basales es 3.5 veces más probable que el enraizamiento de P. leiophylla sea exitoso que utilizando estacas apicales. El tratamiento formado por la mezcla de turba-agrolita-vermiculita, estaca basal y 10,000 ppm de AIB produjo 45.3 % de enraizamiento (mayor porcentaje, mientras que con el testigo solo se obtuvo 8.6 % de enraizamiento (agrolita, estaca basal y sin AIB. Las interacciones que incluyeron el tipo de sustrato mostraron diferencias significativas (P ≤ 0.05 en el crecimiento de la estaca. El uso de agrolita y la aplicación de AIB favorecieron un mayor número de raíces, particularmente en las estacas tipo basal de P. leiophylla.

  17. Regeneration of Rocky Mountain bristlecone pine (Pinus aristata) and limber pine (Pinus flexilis) three decades after stand-replacing fires

    Science.gov (United States)

    Jonathan D. Coop; Anna W. Schoettle

    2009-01-01

    Rocky Mountain bristlecone pine (Pinus aristata) and limber pine (Pinus flexilis) are important highelevation pines of the southern Rockies that are forecast to decline due to the recent spread of white pine blister rust (Cronartium ribicola) into this region. Proactive management strategies to promote the evolution of rust resistance and maintain ecosystem function...

  18. Procesamiento del grano de maíz sobre la cinética de degradación de la materia seca in vitro

    Directory of Open Access Journals (Sweden)

    Raul Velásquez V.

    2013-11-01

    Full Text Available Objetivo. Determinar el efecto del procesamiento del grano de maíz sobre la cinética de degradación y fermentación de la materia seca in vitro. Materiales y métodos. Se evaluaron cinco tratamientos, cada uno constó de una mezcla con dos especies, 70% pasto Kikuyo (Pennisetum clandestinum y 30% grano de maíz (Zea mays. El grano fue sometido a diferentes procesamientos: maíz grueso seco (MGS, maíz fino seco (MFS, maíz reconstituido (MGH, reconstituido y ensilado (MGE y ensilado con urea (MGEU. Se realizó la técnica de gas in vitro para medir parámetros de degradación y de producción de gas en diferentes horarios hasta las 48 h. Se utilizaron los modelos de Gompertz y Ørskov y McDonald para ajustar las curvas de producción de gas y degradación de MS y se evaluaron con PROC NLIN de SAS. Resultados. Los mayores volúmenes acumulados fueron obtenidos con los tratamientos MFS y MGEU con 552.5 ml y 524.03 ml, respectivamente y fueron diferentes al tratamiento MGS (p<0.05. El potencial de degradación (A+B en MGS mostró el menor valor, siendo diferente (p<0.05 a los tratamientos MFS, MGE y MGEU. Conclusiones. El tipo de procesamiento del grano de máiz molido fino, reconstituido, ensilado con y sin urea mejoraron significativamente los parámetros de fermentación y degradación de la MS. El uso de grano molido fino (2 mm puede ser reemplazado por grano grueso reconstituido y ensilado, el cual resulta más económico que la molienda fina.

  19. Optimizaión de un protocolo para el cultivo in vitro y la micropropagación masiva del llantén (Plantago major

    Directory of Open Access Journals (Sweden)

    Giovanni Garro M.

    2009-01-01

    Full Text Available Llantén (P. major posee propiedades medicinales antiinflamatorias y cicatrizantes que han sido aprovechadas en muchas latitudes desde épocas remotas. Los estudios del cultivo in vitro de esta especie son escasos, a pesar de las ventajas que este tipo de técnicas presenta en especies de uso medicinal. En este trabajo se logró desarrollar un protocolo para el establecimiento y la reproducción masiva in vitro del llantén. Se estableció un protocolo de desinfección de semillas y se obtuvo plántulas germinadas in vitro que se utilizaron como fuente de explantes para la fase de micropropagación masiva. Se evaluó el cultivo de ápices en medios de cultivo con sales y vitaminas de M & S (1962 al 100% y al 50%, suplementados con los siguientes reguladores de crecimiento: 1,2mg/l de ANA (Ácido naftalenacético, 0,6 mg/l y 0,4 mg/l de BAP (Bencil amino purina y 1mg/l de TDZ (Tidiazurón en diferentes combinaciones. Los mejores resultados en la inducción de brotes fueron obtenidos en 14,9 brotes promedio por explante en el medio con las sales y vitaminas M & S (1962 reducidas al 50% de su concentración y sin reguladores de crecimiento. Con este tratamiento, un total de 150 vitroplantas provenientes de distintos tratamientos han sido llevadas a condiciones de invernadero con un 100% de sobrevivencia.

  20. Evaluación de daños por patógenos fúngicos en Pinus y Quercus del Área de Protección de Flora y Fauna “La Primavera” Jalisco, México

    OpenAIRE

    Román Jaime Granados; Esteban García Quiñones; Sandra Luz Toledo González; Fidel Góngora Rojas; Luz Elena Claudio Gracía

    2012-01-01

     El Área de Protección de Flora y Fauna “La Primavera” es la masa boscosa con mayor in­fluencia hídrico-ambiental de la zona metropolitana de Guadalajara. En la presente inves­tigación se evaluaron los daños por patógenos fúngicos de Pinus y Quercus, ya que ambos géneros presentan daños visibles. El 93 % de los árboles están dañados; las enfermedades más frecuentes en Pinus son tizón foliar (Lophodermium sp.) y royas de las acículas (Coles­porium sp.); la especie más afectada es P. oocarpa Sh...

  1. Colour Evaluation, Bioactive Compound Content, Phenolic Acid Profiles and in Vitro Biological Activity of Passerina del Frusinate White Wines: Influence of Pre-Fermentative Skin Contact Times

    Directory of Open Access Journals (Sweden)

    Katya Carbone

    2016-07-01

    Full Text Available Passerina del Frusinate is an autochthonous wine grape variety, which grows in the Lazio region that is currently being evaluated by local wine producers. In this study, colour properties (CIELab coordinates, bioactive compounds (total polyphenols and flavan-3-ols, HPLC-DAD phenolic acid profiles and in vitro biological activity of monovarietal Passerina del Frusinate white wines and the effect of different maceration times (0, 18 and 24 h were evaluated based on these parameters. Results highlighted statistically significant differences for almost all analysed parameters due to a strong influence of the pre-fermentative skin contact time. The flavan content of macerated wines was six times higher than that of the control, while total polyphenols were 1.5 times higher. According to their phytochemical content, macerated wines showed the highest antiradical capacity tested by means of DPPH• and ABTS+• assays. Besides, prolonged maceration resulted in a reduction of CIELab coordinates as well as of the content of phenolic substances and antiradical capacity. Among the phenolic acids analysed, the most abundant were vanillic acid and caffeic acid; the latter proved to be the most susceptible to degradation as a result of prolonged maceration. Passerina del Frusinate appears as a phenol-rich white wine with a strong antioxidant potential similar to that of red wines.

  2. Colour Evaluation, Bioactive Compound Content, Phenolic Acid Profiles and in Vitro Biological Activity of Passerina del Frusinate White Wines: Influence of Pre-Fermentative Skin Contact Times.

    Science.gov (United States)

    Carbone, Katya; Fiordiponti, Luciano

    2016-07-22

    Passerina del Frusinate is an autochthonous wine grape variety, which grows in the Lazio region that is currently being evaluated by local wine producers. In this study, colour properties (CIELab coordinates), bioactive compounds (total polyphenols and flavan-3-ols), HPLC-DAD phenolic acid profiles and in vitro biological activity of monovarietal Passerina del Frusinate white wines and the effect of different maceration times (0, 18 and 24 h) were evaluated based on these parameters. Results highlighted statistically significant differences for almost all analysed parameters due to a strong influence of the pre-fermentative skin contact time. The flavan content of macerated wines was six times higher than that of the control, while total polyphenols were 1.5 times higher. According to their phytochemical content, macerated wines showed the highest antiradical capacity tested by means of DPPH(•) and ABTS(+•) assays. Besides, prolonged maceration resulted in a reduction of CIELab coordinates as well as of the content of phenolic substances and antiradical capacity. Among the phenolic acids analysed, the most abundant were vanillic acid and caffeic acid; the latter proved to be the most susceptible to degradation as a result of prolonged maceration. Passerina del Frusinate appears as a phenol-rich white wine with a strong antioxidant potential similar to that of red wines.

  3. Actividad antimicótica in vitro y metabolitos del aceite esencial de las hojas de Minthostachys Mollis (muña

    Directory of Open Access Journals (Sweden)

    Carlos Cano

    2008-07-01

    Full Text Available Objetivos. Demostrar la actividad antimicótica in vitro y la elucidación de algunos de los metabolitos del aceite esencial de hojas de Minthostachys mollis (muña proveniente del distrito de Huacrapuquio (2700 msnm, Junín. Materiales y métodos. El aceite esencial se obtuvo por el método de destilación por arrastre de vapor de agua. Este fue sometido a análisis físico-químico y determinación de la composición química mediante cromatografía de gases. Mediante el método de agar en difusión se evaluó la inhibición del crecimiento fúngico de Candida albicans y también por el método de dilución en tubo para Trichophytun tonsurans, Trichophytun mentagrophytes, Microsporun canis. Resultados. Se encontraron los siguientes monoterpenos: pulegona, mentona, limoneno y mirceno. El aceite esencial de muña inhibió completamente el desarrollo de T. tonsurasn, T. mentagrophytes y M. canis con ambos métodos de evaluación y dosis, para C. albicans se logró un halo de inhibición de 30 mm para el aceite esencial al 100% y de 35 mm al 50%. Conclusiones. Se demostró la actividad antimicótica del aceite esencial de muña, probablemente por la acción de los monoterpenos encontrados.

  4. POTENSI ALELOPAT DAUN PINUS (Pinus spp. SEBAGAI BIOHERBISIDA PRA TUMBUH PADA GULMA KROKOT (Portulaca oleracea

    Directory of Open Access Journals (Sweden)

    Lutfy Ditya Cahyanti

    2015-06-01

    Full Text Available The aim of this experiment was to study the effectof pine leaf as allelophaty on purslane germination. Theexperiment were conducted at screen house Departmentof Biology, Faculty of Mathematics and Science, BrawijayaUniversity. The research is experimental design by nonfactorial Completely Randomized Blok Design, with threereplications, consisted of eleven levels. Purslane seeds sprout with control treatment, signifi cantly different from seedssprout ability in treatment solution leaves Pinus merkusii 2000ppm, and solution leaves of P. longaeva 2000 ppm. The resultshowed that 2000 ppm of P. merkusii extraction signifi cantlysuppressed 46% of purslane germination whereas 2000 ppmPinus longeava extraction signifi cantly suppressed of 41%campared to without any treatments (control.

  5. Estudio in vitro sobre los efectos de la cocaína sobre los tejidos duros del diente In vitro study on the effects of cocaine on the hard tissues of the tooth

    Directory of Open Access Journals (Sweden)

    Eduvigis Solórzano Navarro

    2008-12-01

    Full Text Available El uso de los derivados de la cocaína es un problema de salud pública en continuo crecimiento. Estas drogas son frecuentemente frotadas sobre la mucosa gingival, pero sus efectos son poco conocidos y de difícil diagnóstico. Con este trabajo se pretende demostrar in vitro, que la aplicación por frotación de la cocaína sobre la superficie dentaria produce erosión del esmalte. Se seleccionaron 30 dientes sanos, distribuidos aleatoriamente en 3 grupos, a los cuales se frotó sobre la superficie vestibular clorhidrato de cocaína, basuco y limón (grupo control respectivamente, durante 40 semanas. Las lesiones más características observadas fueron fractura y pérdida de sustancia dura, demostrando así que la aplicación tópica y continua de drogas en cavidad bucal produce lesiones en los tejidos duros del diente.The use of cocaine derivatives is an increasingly growing public health problem. These drugs are frequently rubbed on the gingival mucosa, but their efffects are little known and difficult to be diagnosed. This paper pretends to prove in vitro that the rubbing of cocaine on the dental surface produces enamel erosion. 30 healthy teeth were selected and distributed at random into 3 groups that were rubbed cocaine hydrochloride, bazuko and lemon (control group on the vestibular surface, respectively, during 40 weeks. The most observed characteristic lesions were fracture and loss of the dental hard substance, which showed that the topical and continual application of drugs on the oral cavity causes lesions in the hard tissues of the tooth.

  6. Efecto in vitro del látex de Ficus insipida sobre la cascada de la coagulación sanguínea.

    Directory of Open Access Journals (Sweden)

    Fernando Concha-Benavente

    2010-07-01

    Full Text Available Es necesario investigar drogas naturales nuevas que aporten principios farmacológicos activos para ser utilizadas como una alternativa terapéutica. Por este motivo nos propusimos estudiar a Ficus insipida, cuyo látex ha sido usado como antihelmíntico durante muchos años en la amazonía, pero se investigó solo superficialmente su efecto anticoagulante in vitro. Objetivo: Comprobar el efecto anticoagulante in vitro y determinar la vía de la coagulación sobre la que actúa el látex de Ficus insípida. Material y métodos: Se obtuvo el látex de Ficus insipida y se prepararon diferentes concentraciones del mismo. Se obtuvieron muestras de sangre venosa periférica de 5 donantes voluntarios, anticoagulándolas con citrato de sodio. Luego, éstas se mezclaron con las diluciones del látex, se centrifugaron y se extrajo el plasma. Posteriormente, se obtuvo un pool de plasma para cada concentración del látex y se procedió a determinar el Tiempo de Protrombina (TP y el Tiempo de Tromboplastina Parcial activada (TTPa, respectivamente. Resultados: Se encontró que el látex de Ficus insipida prolongó el TP a una concentración mayor o igual a 0,03125% (V/V, y ambos, el TP y TTPa a una concentración mayor o igual a 0,15% (V/V. Conclusiones: Los resultados obtenidos nos permiten afirmar que el látex de Ficus insipida posee un efecto anticoagulante in vitro dosis dependiente sobre la vía extrínseca de la coagulación sanguínea a una concentración igual o mayor a 0,03125% (V/V y que a una concentración igual o mayor a 0,15% (V/V posee un potente efecto anticoagulante sobre ambas vías de la coagulación.(Rev Med Hered 2010;21:146-152.

  7. Genetic Analysis of Pinus sylvestris L. and Pinus sylvestris forma turfosa L. Using RAPD Markers

    Directory of Open Access Journals (Sweden)

    Beáta ÁBRAHÁM

    2010-03-01

    Full Text Available The purpose of the present study was to determine the level of genetic diversity within and among Ciuc basin, Romania (populations from Mohos and Luci raised bogs in Harghita Mountain and Sumuleu in Ciuc Mountain Pinus sylvestris populations using molecular markers. Two of populations (Mohos and Luci seems to be the descendants that survived the continental glaciation. Genetic diversity was analyzed by RAPD (Random Amplified Polymorphic DNA. Nine primers were selected for analysis, which generated reproducible bands. On base of presence or absence of homologues bands Nei’s gene diversity, the percentage of polymorphic loci and Nei’s unbiased genetic distance were calculated. The level of genetic variation among populations was found to be low. For both populations the variation values among populations were higher than within populations. The fossil records and geological historical data explain the extremely low genetic diversity of this species. Pinus sylvestris experienced strong bottlenecks during its evolutionary history, which caused the loss of genetic variation. Genetic drift and breeding in post-bottlenecked small populations may be the major forces that contribute to low genetic diversity and genetic differentiation of populations. Human activities may have accelerated the loss of genetic diversity in Pinus sylvestris.

  8. utilizando polietileno e serragem de pinus sp

    Directory of Open Access Journals (Sweden)

    Éverton Hillig

    2006-01-01

    Full Text Available In this work, the effect of different compositions of virgin and recycled high density polyethylene/Pinus sp. on physical-mechanical properties of sheets made by compression molding was studied. The compositions were blended in a thermokinetic mixer (Draiss and compression molded at 150oC. ASTM samples for tensile, flexural, impact and hardness tests were taken from the sheets. The density of the sheets were determined. The statistical model used was a centroid simplex with seven compositions and three repetitions. Results showed that tensile and flexural strength as well as hardness and density followed a linear model, while impact strength is explained by a quadratic model. Physicalmechanical properties of compositions using virgin and recycled HDPE did not show significative changes, except for impact strength, when virgin HDPE showed higher numerical results.

  9. Insect-resistant transgenic Pinus radiata.

    Science.gov (United States)

    Grace, Lynette J; Charity, Julia A; Gresham, Belinda; Kay, Nod; Walter, Christian

    2005-05-01

    Transgenic radiata pine (Pinus radiata D. Don) plants containing a Bacillus thuringiensis (Bt) toxin gene, crylAc, were produced by means of biolistic transformation of embryogenic tissue. Using the selectable marker gene nptII and corresponding geneticin selection, 20 independent transgenic lines from five genotypes were established. Over 200 plants regenerated from ten transgenic lines were successfully transferred to soil. The integration and expression of the introduced genes in transgenic tissue and/or plants were confirmed by PCR, Southern hybridisation and neomycin phosphotransferase II (NPTII) and Bt ELISA assays. Bioassays with larvae of the painted apple moth, Teia anartoides, demonstrated that transgenic plants displayed variable levels of resistance to insect damage, with one transgenic line being highly resistant to feeding damage.

  10. Fire effects in Pinus uncinata Ram plantations

    Directory of Open Access Journals (Sweden)

    Adrián Cardil Forradellas

    2016-04-01

    Full Text Available Aim of study: Understanding fire ecology of main forest species is essential for a sound, scientifically based on managing of wildlands and also to assess likely implications due to changes in fire regime under a global change scenario. Few references can be found about fire ecology of Pinus uncinata Ram. (PU. PU species grows in the Central Pyrenees where large, severe wildland fires did not occur frequently in the past. However, several fires with extreme fire behavior have affected PU stands in last years and they might disturb other PU forest in the future.Area of study: Cabdella fire (February 2012, in Lleida province, is one of the several wildland fires occurred in 2012 (winter season in the Central Pyrenees. Fire affected a large PU plantation (102 ha located at 1.800-2,100 meters above the sea.Material and methods: We have analyzed first order fire effects in three fireline intensity thresholds along three years in terms of mortality ratio, scorched height, percentage of scorched crown volume and bark char height.Main results: PU seems to be a very tolerant species to low and medium fire line intensity but fire effects were very significant when fire line intensity was high. In medium fireline intensity sites, probability of mortality ranged from 15 to 30% and the dead trees had the highest values on scorched height and percentage of scorched crown volume.Research highlights: Results from this work supports that prescribed burning might be used to efficiently decrease fuel load and fuel vertical continuity while avoiding considerable PU mortality. It also displayed that when fuel management has been implemented, PU mortality might be limited even under extreme fire behavior.Abbreviations used: PU: Pinus uncinata Ram.

  11. Aislamiento de una cepa de campo de Babesia bigemina (Piroplasma: Babesiidae y establecimiento del cultivo in vitro para la producción de antígenos

    Directory of Open Access Journals (Sweden)

    Roger I Rodríguez-Vivas

    2007-03-01

    Full Text Available La babesiosis bovina ocasionada por Babesia bigemina produce grandes pérdidas a la economía pecuaria Mexicana. Debido a las variaciones antigénicas que presenta B. bigemina es necesario contar con antígenos locales para la realización de estudios epidemiológicos y de inmunización. Se recolectaron 30 garrapatas Boophilus microplus de un bovino adulto clínicamente enfermo de babesiosis (B. bigemina en Yucatán, México. En laboratorio se produjeron larvas, ninfas y una cepa congelada. El aislamiento de la cepa se realizó con éxito y se necesitó 30 días en el cultivo in vitro para obtener un PEI del 1.5 %. La cepa fue congelada en nitrógeno líquido y reactivada en el cultivo celular. La concentración del medio que permitió el mayor PEI (14 %, pIsolation of a field strain of Babesia bigemina (Piroplasma: Babesiidae and establishment of in vitro culture for antigen production. Bovine babesiosis, caused by Babesia bigemina, is a barrier for livestock development; it results in high economic loss to Mexican livestock. Control requires adequate antigens for diagnosis and vaccination programs. However, because of antigenic variation among Babesia strains, it is necessary to use antigens prepared from local strains. The purpose of the present study was to isolate a local field strain and to establish the in vitro culture of B. bigemina by the evaluation of the constituent’s concentration of culture media. Thirty engorged female Boophilus microplus were collected from cattle suffering clinical babesiosis (B. bigemina in Yucatan state, Mexico. These ticks were sent to the laboratory for detection of Babesia sp. vermicules. Eggs were kept at 83-85 % humidity and 27 ºC until hatching. Larvae were transferred to an esplenectomized calf (B-1. The resulting nymphs were transferred to an esplenectomized calf (B-2. Twelve days later, B. bigemina (local strain was detected in calf B-2 and its infected blood was frozen in liquid nitrogen to

  12. Determinación In Vitro del factor de protección Ultravioleta en las telas producidas en el Ecuador

    OpenAIRE

    Romero Feijoo, Bertha Elizabeth

    2012-01-01

    Objetivo: Determinar el grado de protección a la radiación ultravioleta que ofrecen los tejidos fabricados y comercializados en el Ecuador. Metodología: Estudio descriptivo y analítico que incluyo la utilización de 2 métodos: in vitro e in vivo, y una muestra de 72 telas y en el método in vivo 10 personas. Se utilizó un equipo denominado espectrofotómetro que emite radiación con un rango en nm y que mide la transmitancia a través del tejido. Análisis Estadístico: La representación de lo...

  13. EFECTO DEL SUSTRATO, SOBRE LA ACTIVIDAD ACETOGÉNICA IN VITRO DE Ruminococcus schinkii EN INTERACCIÓN CON HONGOS DEL RUMEN

    Directory of Open Access Journals (Sweden)

    Miramontes Carrillo JM

    2011-01-01

    Full Text Available La acetogénesis es una alternativa para inhibir el metano del rumen. Se evalúo el efecto del sustrato, sobre la actividad acetogénica de Ruminococcus schinkii con hongos. Se cultivaron bajo anaerobiosis forrajes molidos en medios más hongos y acetogénicas. Se formaron siete grupos de 24 botellas con medio, sustrato y 2-ABS. Seis grupos tuvieron esporas de hongos y acetogénicas. Tres grupos con forraje, sin microorganismos, fueron blancos. El diseño fue factorial 3x2x6; A=3 forrajes; B= hongos y hongo más acetogénica y C=6 tiempos de incubación. Cada tratamiento se realizó con cuatro repeticiones en tres periodos. El sustrato afectó DMS, la producción de azúcares reductores y pH (p<0.0001 por la actividad acetogénica y hongos. DMS del maíz y sorgo fue mayor que la alfalfa. La alfalfa produjo más azúcares reductores. El acetato no fue afec- tada por los sustratos (p=0.3417, pero el pH presenta diferencias. Factor microorganismos presenta efectos por los sustratos. La actividad acetogénica mayor en cocultivo con hongos y bacterias (p<0.0001. El tiempo de incubación afectó todas las variables. DMS para el maíz fue mayor a las 96 h, el sorgo 144 y alfalfa 124 (p<0.0001. El acetato fue mayor para todos los sustratos a las 120 h (p<0.0001, producción de azúcares fue mayor para todos los sustratos a las 144 h (p<0.0001 y pH a las 0 h para todos los sustratos (p<0.0001. La capacidad de los hongos para degradar las vegetales se potencializa en interacción con acetogénicas, permi- te una mayor DMS, una mayor producción de acetato y una inhibición de la metanogénesis.

  14. Efecto in vitro del D-002 sobre la actividad enzimática de la 5-lipoxigenasa (5-LOX Effect of D-002 on 5-lipooxygenase (5-LOX enzyme activity in vitro

    Directory of Open Access Journals (Sweden)

    Yohani Pérez Guerra

    2012-06-01

    Full Text Available Introducción: el D-002, mezcla de seis alcoholes alifáticos primarios de alto peso molecular purificada de la cera de abejas, ha mostrado efectos antiinflamatorios, sin gastrotoxicidad secundaria, enmodelos experimentales. El tratamiento oral con D-002 reduce las concentraciones de leucotrieno B4 (LTB4 en exudados pleurales de ratas con pleuresía por carragenina, sugiriendo que podría inhibir la actividad enzimática de la 5-lipooxigenasa (5-LOX, si bien los mecanismos de la acción antiinflamatoria del D-002 no habían sido aún explorados. Objetivo: evaluar el efecto in vitro del D-002 sobre la actividad de la 5-LOX, utilizando la fracción citosólica de homogenatos de hígado de ratas. Métodos: las condiciones de ensayo utilizadas fueron las siguientes: fracción citosólica (50 µg de proteína disuelta en solución reguladora borato 0,2 mol/L (pH 9 y ácido linoleico (7,8-250 mmol/L como sustrato, ensayándose muestras paralelas incubadas con Tween-20/H2O (2 % (vehículo, muestras controles, D-002 (0,9-1 000 µg/mL o Lyprinol (500 µg/mL (sustancia de referencia. La actividad enzimática se evaluó mediante el cambio de absorbancia a 234 nm producido por la formación de dienos conjugados y medido en espectrofotómetro UV-visible. Resultados: la adición in vitro del D-002 produjo una inhibición significativa, dependiente de la dosis (r= 0,980; pIntroduction: D-002, a mixture of six high molecular weight primary aliphatic alcohols purified from beeswax, has been shown to produce anti-inflammatory effects with no secondary gastrotoxicity in experimental models. Oral treatment with D-002 was effective for lowering the concentrations of B4 leukotriene (LTB4 in pleural exudates of rats with carragenin-induced pleurisy, suggesting that it could inhibit 5-lipooxigenase (5-LOX enzyme activity. The mechanisms of the anti-inflammatory action of D-002, however, had not been explored yet. Objective: to evaluate the effects of D-002 on 5-LOX

  15. Susceptibility of three clinical isolates of Actinomodura madurae to α-pinene, the bioactive agent of Pinus pinaster turpentine oil

    Directory of Open Access Journals (Sweden)

    Stojković D.

    2008-01-01

    Full Text Available In vitro susceptibility of the turpentine oil obtained from Pinus pinaster oleoresin was evaluated against three Sudanese clinical isolates of Actinomadura madurae, which is the main causative agent of actinomycetoma. The minimum inhibitory concentrations (MICs of the oil ranged from 100.3 to 124.8 μL/mL, and the minimum microbicidal concentrations (MMCs were between 100.3 and 150.0 μL/mL. α-Pinene exhibited prominent bioactivity with MICs ranging between 3.3 and 5.0 μL/mL, while its MMC was 10.0 μL/mL against the same clinical isolates. Pinus pinaster turpentine oil and α-pinene might be useful agents in the treatment of mycetoma caused by A. madurae.

  16. Various response of Pinus tabulaeformis Carr. regeneration in artifical gaps

    National Research Council Canada - National Science Library

    Zhibin Wang; Kuangji Zhao; Haijiao Yang; Lvyi Ma; Zhongkui Jia

    2017-01-01

    ... (Pinus tabulaeformis Carr.) plantation to test the short-term effects of gap size on the age distribution, density and growth, and annual height and ground diameter growth for regeneration established before (REBG) and after (REAG) gap creation...

  17. Propiedades antibacterianas in vitro de metabolitos secundarios aislados de dos especies del género Zanthoxylum (Rutaceae)

    OpenAIRE

    Oscar Javier Patiño Ladino; Juliet Angélica Prieto Rodríguez; José Manuel Lozano Moreno; Liliana Lesmes Sarmiento; Luís Enrique Cuca Suárez

    2011-01-01

    Actualmente las enfermedades infecciosas originadas por diferentes microorganismos, son una de las mayores causas de muerte a nivel mundial, razón por la que la búsqueda de nuevos agentes antimicrobianos se ha hecho indispensable, y los productos naturales han constituido una de las fuentes potenciales para encontrar compuestos antimicrobianos. Las especies del género Zanthoxylum se caracterizan por presentar diversos tipos de metabolitos secundarios, muchos de los cuales han mostrado interes...

  18. Estudio de medidas de control de la colonización microbiana de la cámara interna del implante y del sistema implante-pilar por medio de un estudio in vitro

    Directory of Open Access Journals (Sweden)

    Pablo Julio Pebé

    Full Text Available Resumen: Objetivo: Determinar mediante un ensayo in vitro, medidas de control del biofilm en la cámara interna del implante. Método: Se seleccionaron diferentes agentes antimicrobianos que se colocaron en las cámaras de 3 grupos de implantes. Luego de 7 días de inmersos en una suspensión microbiana e incubados, realizamos la toma, cultivo e incubación de las muestras de las cámaras de cada grupo de estudio. Resultados: Se constató una filtración de microorganismos hacia la cámara interna en todos los grupos de implantes estudiados, obteniéndose un mayor recuento de Unidades Formadoras de Colonias (UFC en el grupo control, mientras que en los grupos experimentales se identificó una reducción significativa en el recuento de UFC. Conclusiones: Se observó una disminución significativa en la cantidad de UFC en los grupos experimentales respecto al grupo control, lo que determina la ventaja de utilizar este tipo de antimicrobianos

  19. Evaluación in vitro del ensilaje de girasol (Helianthus annuus L. solo y combinado con ensilaje de maíz

    Directory of Open Access Journals (Sweden)

    Ramón Gonzalo Aragadvay-Yungán

    2015-01-01

    Full Text Available El ensilado de girasol forrajero (Helianthus annuus L. es promovido para la alimentación del ganado lechero anteescasez de riego y lluvias erráticas. El objetivo fue valorar al ensilado de girasol, solo y en diferentes proporcionescon ensilado de maíz mediante degradación in vitro y la cinética de producción de gas. Se realizaron micro-silos,abiertos a los 80 días para el ensilado de maíz (EMz y 50 días para el ensilado de girasol (EG, evaluándoselos siguientes tratamientos (EMz%: EG%; T1 100:0, T2 75:25, T3 50:50, T4 25:75 y T5 0:100. En el T2 la fibradetergente neutra y ácida disminuyeron significativamente (P0.05 similar a T1. Hubo mayor producción de gas total en T1, pero con una menor tasa de fermentación(P<0.05 al compararlo con T2 cuya tasa de fermentación fue de 0.0530/h, con un tiempo Lag de 3.4 h. El girasolaporta 31.8 % más de PC, 11.8 % menos de energía metabolizable y 11.9 % menos de digestibilidad in vitro.Comparado con el T1, el T2 aportó 1.6 % más de proteína, 2 % menos de energía metabolizable y 4.4 % menosde digestibilidad in vitro. Se concluye que el ensilado de girasol podría ser una alternativa para sustituir alensilado de maíz hasta un 25 %, ya que el aporte de proteína y energía es similar al ensilado de maíz solo.

  20. Probability of mortality by fire damage of young Pinus hartwegii Lindl. trees in the Izta-Popo National Park

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    César A. Robles-Gutiérrez

    2016-01-01

    Full Text Available A nivel mundial se realizan grandes esfuerzos para determinar el efecto del fuego en la mortalidad de especies arbóreas como las del género Pinus . En este trabajo se evaluó la inf luencia del fuego en la probabilidad de mortalidad del arbolado joven de Pinus hartwegii en el Parque Nacional Izta-Popo, área natural protegida del centro de México. Los efectos de un incendio de mediana a alta intensidad ocurrido en marzo del 2013, una quema prescrita de baja intensidad realizada en abril del mismo año y un área testigo sin presencia de fuego reciente se valoraron y compararon. Los resultados mostraron que 18 meses después de aplicados los tratamientos, la mortalidad del arbolado en el área con quema prescrita fue de 13 %, mientras que en el área incendiada y testigo fue de 28 y 4 %, respectivamente. Se obtuvieron seis modelos logísticos para predecir la mortalidad; el porcentaje de copa dañada, la altura de cicatriz y el diámetro normal con corteza fueron las variables significativas ( P < 0.05 de predicción. Los resultados sugieren que la disminución en la capacidad fotosintética y en el transporte de nutrimentos, asociados al daño de la copa y al cámbium, están estrechamente ligados con la probabilidad de mortalidad.

  1. Recidiva del color dentario por té, café y vino: In vitro Dental bleaching regression caused by chromogenic beverages: In vitro

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    M Arévalo Pineda

    2012-08-01

    Full Text Available Este estudio, in vitro, determinó si los dientes con clareamiento presentan mayor cambio de color en el tiempo que los no tratados, al someterse a tinción con bebidas cromógenas, café, té y vino. Se utilizaron 45 incisivos sanos de bovino conservados en suero a 37ºC. Cada espécimen se dividió en dos mitades, una sometida a clareamiento con peróxido de hidrógeno al 35% y otra control. Se midió color con Espectrofotómetro Vita EasyShade. Se dividieron los especímenes al azar en grupos de 15 y fueron sumergidos en café, té y vino, durante 10 minutos, 20 veces, registrando color después de cada inmersión. Los datos fueron analizados con ANOVA y Test de Tukey, con 95% de intervalo de confianza. Los resultados mostraron que, el clareamiento modifica significativamente (p=0.05 el color en los tres grupos (GC=85.8 a 95.1; GT=87.4 a 97.3 y GV=90.8 a 99.3, la recidiva de color se observa a lo largo de las 20 inmersiones, siendo significativa la diferencia de valores ΔE iniciales (GC=18.89; GT=22.97; GV=56.46 y finales (GC=5.56; GT=5.38; GV=12.49. El grupo tratado presenta mayor descenso de unidades ΔE a lo largo de las inmersiones, por lo que es el más teñido (GCcontrol=20.98-5.01; GTcontrol=17.11-3.66; GVcontrol=54.62-11.49. Las tres bebidas cromógenas causan recidiva de color en los dientes clareados, siendo el vino el que causa mayor tinción. Se concluyó que las piezas tratadas, sometidas a los tres tipos de cromógenos, tienen mayor cambio de color que las que no lo son, pero finalmente no se oscurecen más que las no tratadas.This in vitro study established if teeth treated with dental bleaching have a higher change of color over time than those that aren’t treated, when subjected to three chromogenic beverages (coffee, tea and red wine. 45 healthy bovine incisors were used, maintained in 0.9% sodium chloride, at 37ºC. Every specimen was divided into 2 half; one half was subjected to dental bleaching with 35% hydrogen

  2. Propiedades antibacterianas in vitro de metabolitos secundarios aislados de dos especies del género Zanthoxylum (Rutaceae

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    Oscar Javier Patiño Ladino

    2011-09-01

    Full Text Available Actualmente las enfermedades infecciosas originadas por diferentes microorganismos, son una de las mayores causas de muerte a nivel mundial, razón por la que la búsqueda de nuevos agentes antimicrobianos se ha hecho indispensable, y los productos naturales han constituido una de las fuentes potenciales para encontrar compuestos antimicrobianos. Las especies del género Zanthoxylum se caracterizan por presentar diversos tipos de metabolitos secundarios, muchos de los cuales han mostrado interesantes propiedades biológicas contra diversos microorganismos patógenos humanos. El objetivo de esta investigación consistió en evaluar la actividad antibacteriana de 21 sustancias aisladas de 2 especies del género Zanthoxylum (Rutaceae. La actividad fue determinada por el método de difusión radial en agar contra cepas de bacterias estándar grampositivas: Staphylococcus aureus ATCC 6538 y Enterococcus faecalis ATCC 29212 y gramnegativas: Escherichia coli ATCC 25922 y Salmonella tiphymurium, cepas ATCC 14028s y MS7953. De las 21 sustancias naturales evaluadas, 11 causaron algún grado de inhibición del crecimiento bacteriano. El alcaloide benzofenantridínico queleritrina presentó la mayor actividad, e inhibió el crecimiento de las 5 cepas con halos de inhibición comparables o superiores a los mostrados por los antibióticos empleados como controles positivos. Los alcaloides isoquinolínicos en general, son las sustancias más promisorias para continuar con estudios más específicos de actividad antibacteriana, pues además de presentar una actividad interesante, algunas de estas sustancias inhiben tanto el crecimiento de bacterias grampositivas como el de bacterias gramnegativas, lo cual indica que son sustancias que presentan actividad antibacteriana de amplio espectro.

  3. Evaluación de daños por patógenos fúngicos en Pinus y Quercus del Área de Protección de Flora y Fauna “La Primavera” Jalisco, México

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    Román Jaime Granados

    2012-07-01

    Full Text Available  El Área de Protección de Flora y Fauna “La Primavera” es la masa boscosa con mayor in­fluencia hídrico-ambiental de la zona metropolitana de Guadalajara. En la presente inves­tigación se evaluaron los daños por patógenos fúngicos de Pinus y Quercus, ya que ambos géneros presentan daños visibles. El 93 % de los árboles están dañados; las enfermedades más frecuentes en Pinus son tizón foliar (Lophodermium sp. y royas de las acículas (Coles­porium sp.; la especie más afectada es P. oocarpa Shiede ex Schltdl; en Quercus los pade­cimientos más comunes son manchas foliares (Pestalotia sp., cánceres y tumores en los fustes, siendo la especie más dañada Q. magnoliifolia Née. Al tratarse de un área protegida, se recomienda establecer un sistema de monitoreo fitopatológico permanente de las espe­cies ecológicamente importantes para conocer la dinámica de las enfermedades presentes y establecer acciones de manejo fitopatológico apropiadas, ello para contar con garantía con ecosistemas sanos en el área natural protegida.

  4. Cambios morfológicos en gametos del barbo tigre Puntius tetrazona (Cypriniformes: Cyprinidae e implementación de la fertilización in vitro

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    Omar Domínguez-Castanedo

    2014-12-01

    Full Text Available El objetivo del presente estudio fue conocer las características de los gametos de Puntius tetrazona (n=240, los cambios morfológicos a partir de su activación mediante espermogramas cásicos y por otro lado, se evaluó la implementación de la fertilización in vitro a partir de la tasa ovulatoria, el % de fertilización y eclosión y el número de larvas vivas a las 72h. El volumen promedio de semen fue de 3.0±0.7µL. La concentración espermática mínima, máxima y promedio fue 44.48x10(6spz/mL, 52.3x10(6spz/mL y 48.1±5.9x10(6spz/mL, respectivamente. La viabilidad promedio fue de 84.68±3.27%. La motilidad promedio fue 81.53±2.28%. El diámetro de los espermatozoides fluctuó entre 2.16±0.2 y 2.79±0.3µm; 3.84±0.3 y 4.86±0.31µm sin y con contacto con el agua respectivamente, con diferencias significativas. El diámetro mayor fue a los 30s en contacto con el agua. Los ovocitos de menor y mayor diámetro se registraron a los diez y 30s sin y con contacto con el agua respectivamente. Los diámetros de los ovocitos en diez y 30s en contacto con el agua fluctuaron entre 1.11 y 1.55mm respectivamente. La mayor tasa ovulatoria fue en la fertilización in vitro con 250±50 ovocitos frente a 28±09 de la natural, con diferencias significativas. Los porcentajes de fertilización y eclosión fueron más elevados en la fertilización natural con 80% y 60% respectivamente. Se registraron 75±18 larvas a las 72 horas en el grupo in vitro comparado con 13.4±12 larvas de la fertilización natural. Con lo anterior, la técnica que permitió mayor cantidad de larvas fue la de fertilización in vitro.

  5. Pinus cembra distribution in the Romanian Carpathians

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    Ioan Blada

    2008-09-01

    Full Text Available A map with the distribution of the Swiss stone pine (Pinus cembra L. in Romania was never made. However, long time ago, some poor or confused data collected from the Romanian Carpathians were inserted in a compiled at European level map; but this map do not reflects the species real geographic distribution in the Romanian Carpathians. Consequently, a initiative to build up a not to far from realitymap was made. A comprehensive literature and field survey concerning Swiss stone pine natural distribution in Romania was made. Two other sources of information were used, such as: the senior author's direct field survey to localize the species populations and information obtained from people involved in this issue. Based on the acquired data, the species digital distribution map of the Romanian Carpathians, was in premiere made. Because the distribution boundary of the species encompasses a large area, it does not mean that all hachured mountains and valley on the map are fully occupied by species.

  6. Antagonismo de Trichoderma SPP. E Bacillus subtilis (UFV3918 a Fusarium sambucinum em Pinus elliottii engelm

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    Caciara Gonzatto Maciel

    2014-06-01

    Full Text Available Pinus elliottii é uma espécie de importância no setor florestal e apresenta vulnerabilidade na qualidade sanitária de suas sementes, especialmente pela associação de Fusarium spp., responsável por perdas de plântulas no viveiro. Este trabalho teve como objetivo avaliar a ação antagonista in vitro e in vivo dos agentes Trichoderma spp. e Bacillus subtilis (UFV3918 no controle de Fusarium sambucinum, responsável por danos em plântulas de Pinus elliottii. O controle in vitro foi avaliado através da inibição do crescimento micelial (confronto pareado de culturas, após a incubação a 25±2 ºC e fotoperíodo de 12 h. Para os testes in vivo (desenvolvidos em condições de viveiro, as sementes inicialmente foram inoculadas com o patógeno e, na sequência, microbiolizadas com os agentes antagônicos, para posterior semeadura. Utilizaram-se as técnicas de contato com o biocontrolador em meio BDA por 48 h e peliculização, como formas de microbiolização. Tanto Trichoderma spp. quanto Bacillus subtilis (UFV3918 foram eficientes no controle in vitro de F. sambucinum, e no teste de biocontrole in vivo o produto Bacillus subtilis (UFV3918 destacou-se, reduzindo as perdas de plântulas causadas pelo patógeno, assim como potencializando as variáveis de comprimento de plântula, massa verde e massa seca.

  7. Morphological and physiological damage by surfactant-polluted seaspray on Pinus pinea and Pinus halepensis.

    Science.gov (United States)

    Nicolotti, Giovanni; Rettori, Andrea; Paoletti, Elena; Gullino, Maria Lodovica

    2005-06-01

    This paper reports morphological and physiological damage caused by polluted seaspray to coastal pine forests in Liguria (Northern Italy) and suggests the most reliable parameters for surfactant-pollution biomonitoring. Concentrations of surfactants in surface seawater, seaspray, and that deposited on Pinus halepensis and Pinus pinea needles were determined in samples from five sites. Decline of the pines in the Western part of Liguria was greater than in the East, and was associated with higher surfactant levels deposited on the crowns. Chloride content of needles was higher in damaged pines, even if it did not reach toxic levels. Stomata micromorphologies did not differ between species in the crown parts facing the sea, while differences were significant in the back crown parts that were not directly exposed to polluted sea breezes. Water content and noon water potential indicated interference in water relations of damaged trees. In conclusion, none of the investigated parameters was by itself a comprehensive index of surfactant damage. A simultaneous survey of several parameters is suggested to investigate the impact of surfactants on coastal vegetation. The most useful parameters were: directionality of crown damage, surfactant depositions on the needles, chloride accumulation in the needles, structural injury to epistomatal chambers, needle water content and potential.

  8. Fusariose em Mudas de Pinus taeda Fusarium disease on Pinus taeda seedlings

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    Albino Grigoletti Júnior

    2011-03-01

    Full Text Available

    Viveiros comerciais têm apresentado mudas de Pinus taeda com sintomas de murcha e seca de ponteiros e morte, na Região Sul do Brasil. Isolamento em meio BDA e câmara úmida, teste de patogenicidade e microcultivo foram feitos para identificar o patógeno. Uma espécie de Fusarium foi isolada, cuja identificação encontra-se em andamento. Verificou-sepelos postulados de Koch que Fusarium sp. foi o agente causal dessa doença.
    Nurseries has presented Pinus taeda seedling with symptoms of wilt, tip blight and death, in Southern Region of Brazil. Isolation on PDA medium, moist chamber, pathogenicity test and microculture were made to identify the pathogen. A species of Fusarium was isolated, which is under identification. It was verified by Koch postulates that Fusarium sp. was the causal agent of this disease.

  9. Primer registro de Naupactus ruizi (Coleoptera: Curculionoidea asociado con Pinus ponderosa (Gymnospermae: Pinaceae en Patagonia

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    Cecilia A. GOMEZ

    2006-01-01

    Full Text Available Naupactus ruizi (Brèthes es un gorgojo de rostro corto, distribuido en la Argentina y Chile, que habita en ambientes áridos asociado con vegetación xerofítica y alcanza el rango más austral entre los miembros de la tribu Naupactini. Un relevamiento de insectos y de patógenos como plagas potenciales, realizado durante el 2005 en plantaciones de Pinus spp. en la Patagonia andina argentina, resultó en el hallazgo de adultos de N. ruizi que se alimentaban de acículas de Pinus ponderosa Dougl. ex Laws. Este hallazgo es sorprendente dado que las especies de Naupactini, consumen casi exclusivamente angiospermas. Interpretamos que el cambio de huésped, habría ocurrido como consecuencia de una colonización reciente favorecida por la amplia distribución geográfica del gorgojo y su capacidad para sobrevivir en hábitats marginales, donde probablemente las plantas nativas son escasas y el nuevo huésped presenta una gran abundancia local.

  10. Agua residual y complementada como sustituto del medio de cultivo "in vitro" de embriones de café

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    Ramos, RA.

    2001-01-01

    Full Text Available This work was carried out in Provincial Enterprise from Hydraulicai Resources of Santiago de Cuba and a Genetic Laboratory from Central Research Station of Coffee and Cacao, Tercer Frente, Santiago de Cuba province, from February to June of 1996 period. The "in vitro" culture of Coffea arabica L. 'Catuai Rojo' var. embryos was made as substitute the traditionally culture media using wastewater of Neat Enterprise of Santiago de Cuba as alone and mixed form with coconut milk and ammonium nitrate. Main chemical components were analysed at 19, 27, 36 and 44 days after culturing, the stem and root length were evaluated. The statistical analysis showed a significative difference between treatments. The best behaviour culture media were wastewater complemented with 500 mg. l1 or 1000 mg. V of ammonium nitrate.

  11. Polyphenol-rich extract from Pinus sylvestris L. bark--chemical and antitumor studies.

    Science.gov (United States)

    Amalinei, Roxana Laura Mihailescu; Trifan, Adriana; Cioanca, Oana; Miron, S D; Mihai, C T; Rotinberg, P; Miron, Anca

    2014-01-01

    Pinus sylvestris L. is a very important timber tree in Romania, the bark being the main waste from the wood processing. The aim of this study was to investigate the chemical composition and antitumor effects against HeLa cells of a polyphenol-rich extract from Pinus sylvestris L. bark. The polyphenolic content and profile were analyzed by means of spectrophotometry and RP-HPLC-UV/ESI-MS in the negative ion mode. The antitumor activity was investigated using two in vitro assays: MTT and flow cytometric apoptosis assays. Pine bark extract contained a high total phenolic content (48.16 +/- 0.29%). RP-HPLC-UV/ESI-MS analysis allowed the identification of taxifolin, a taxifolin-hexoside and several procyanidins (two monomers, three dimers and three trimers). At 200 microg/mL, pine bark extract exhibited a high cytotoxicity against HeLa cells (82.10 +/- 1.95%). Flow cytometry revealed the ability of pine bark extract to induce apoptosis: 71.97 +/- 0.96% apoptotic cells were determined in HeLa cells after a 48 h incubation with pine bark extract (200 microg/mL). The ability of pine bark extract to reduce viability and induce apoptosis in HeLa cells suggests the presence of compounds with antitumor properties and encourages further studies for their isolation and characterization.

  12. Avaliação da qualidade de painéis compensados de Pinus maximinoi, Pinus oocarpa e Pinus tecunumannii com diferentes composições estruturais

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    Setsuo Iwakiri

    2012-06-01

    Full Text Available http://dx.doi.org/10.5902/198050985738Este trabalho teve por objetivo avaliar a influência de diferentes composições estruturais sobre as propriedades mecânicas de painéis compensados de Pinus maximinoi, Pinus oocarpa e Pinus tecunumannii. Foram produzidos em laboratório painéis compensados com sete lâminas de 3,0 mm de espessura, com composição padrão, reforço longitudinal e reforço transversal. Para a colagem de lâminas foi utilizada resina fenol-formaldeído com a seguinte formulação em partes por peso: resina = 100, extensor = 10 e água = 10. A gramatura empregada foi 160 g/m² (linha simples. Os painéis foram prensados à temperatura de 150ºC, pressão específica de 15 kgf/cm² e tempo de prensagem de 15 minutos. Para avaliação das propriedades mecânicas foram realizados os ensaios de flexão estática paralela e perpendicular à direção das fibras das lâminas das capas. Os painéis de Pinus maximinoi apresentaram resultados médios superiores de MOE e MOR paralelo e perpendicular em relação aos painéis de Pinus oocarpa e Pinus tecunumannii. Os painéis produzidos com as três espécies estudadas apresentaram resultados satisfatórios de MOE e MOR, paralelo e perpendicular, em comparação aos painéis compensados comerciais produzidos de Pinus taeda. As inclusões de reforço longitudinal e transversal não influenciaram significativamente nos resultados médios de MOE e MOR paralelo. O reforço longitudinal afetou negativamente os resultados médios de MOE e MOR perpendicular.

  13. Crocus sativus, Serenoa repens and Pinus massoniana extracts modulate inflammatory response in isolated rat prostate challenged with LPS.

    Science.gov (United States)

    Chiavaroli, A; Recinella, L; Ferrante, C; Locatelli, M; Carradori, S; Macchione, N; Zengin, G; Leporini, L; Leone, S; Martinotti, S; Brunetti, L; Vacca, M; Menghini, L; Orlando, G

    Prostatitis is a common prostate disease that could be promoted by bacterial or non-bacterial infectious agents. In addition, inflammatory pathways involved in prostatitis have been increasingly studied, and herbal extracts endowed with anti-inflammatory effects are under investigation, individually or in combination, for their efficacy in alleviating the burden of inflammation, with possible improvements in symptoms. Serenoa repens (Serenoa), in combination with Crocus sativus (Crocus) and Pinus massoniana (Pinus), has previously shown to improve sexual function and limit urinary symptoms in patients suffering from concomitant erectile dysfunction and lower urinary tract symptoms. In this context, the aim of the present study is to evaluate the efficacy of Serenoa, Crocus and Pinus extracts, either alone or in combination, on immortalized prostate cells (PC3) and in an experimental model of bacterial prostatitis constituted by ex vivo prostate specimens challenged with lipopolysaccharide (LPS). We found that the tested extracts were able to reduce ROS production by PC3 cells and NFkB and PGE2 activity in prostate specimens challenged with LPS. In addition, the pharmacological association of the extracts displayed synergistic effects indicating a rational use of the mixture of the tested extracts as a novel anti-oxidant and anti-inflammatory formulation in bacterial prostatitis. Finally, we performed analytical and in vitro evaluation to better characterize the phytochemical profile and the mechanism of action of selected secondary metabolites.

  14. Bio-engineering traits of Pinus radiata D.Don

    Science.gov (United States)

    Giadrossich, Filippo; Marden, Michael; Marrosu, Roberto; Schwarz, Massimiliano; Phillips, Chris John; Cohen, Denis; Niedda, Marcello

    2017-04-01

    Pinus radiata is widely cultivated in New Zealand. Due to steep slopes and intense rainfall, the silviculture of Pinus radiata forests is important to control erosion and slope stability. Bio-engineering traits such as root distribution and root tensile strength are fundamental to understand the effectiveness of Pinus radiata. This information is needed to use the state of the art root reinforcement model (the Root Bundle Model) and the physically-based slope stability model SOSlope. Yet, little is known about root distribution and tensile strength for this specie. We measured soil moisture and carried out 30 field tensile tests on roots of Pinus radiata. We also measured root distribution data from 5 plants, digging arc of circles 0.6 radian around the trees in four opposite directions. We fully excavated the root system of two trees. Using the Root Bundle Model, results of our measurements allow estimation of root reinforcement. With the slope stability model SOSlope, information on the intensity and frequency of harvesting and on the development of weak zones that can be supported by a stand of Pinus radiata in relation to slope stability can be calculated. An added value is that the collected data allow us to make inferences between number and sizes of roots, and growth direction.

  15. Caracterización del perfil de sensibilidad de un panel de líneas celulares para valoración de citotoxicidad in vitro.

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    Claudia Jhoana León

    2006-03-01

    Full Text Available Introducción. La valoración de la citotoxicidad in vitro de líneas celulares derivadas de tumores humanos se emplea como bioensayo preliminar para el tamizaje de productos de origen natural con potencial actividad anticancerígena. Objetivo. Fortalecer el modelo de valoración de citotoxicidad in vitro disponible en el laboratorio, ampliando el panel de líneas celulares y caracterizando su perfil de sensibilidad a los fármacos antineoplásicos. Materiales y métodos. Se adicionaron al panel las líneas celulares HeLa, MKN-45 y U-937, y se evaluó la sensibilidad de las siete líneas celulares (HEp-2, HT-29, MCF-7, SiHa, MKN-45, HeLa y U-937 a los fármacos antineoplásicos doxorrubicina HCl, taxol, cisplatino, ciclofosfamida y carmustina, usados en la terapia antineoplásica. Para la valoración de la citotoxicidad se empleó el método de reducción del metil-tiazol-tetrazolio. Resultados. Al comparar las concentraciones letales 50 (CL50 calculadas, se evidenció una sensibilidad diferencial de las líneas celulares frente a doxorrubicina HCl, taxol y cisplatino, siendo HEp-2 la línea más sensible a todos los fármacos, en tanto que las HeLa y U-937 fueron las más resistentes. La respuesta de HEp-2 frente al taxol presentó un comportamiento bifásico, relacionado con su mecanismo de acción. Conclusión. En las condiciones empleadas no se observaron efectos frente a la ciclofosfamida y la carmustina.

  16. Estudio in vitro de la influencia del heterodímero de los receptores D2 de dopamina y 5-HT2A de serotonina, en aplicación a la farmacoterapia antipsicótica

    OpenAIRE

    Gómez Fernández, Sonia

    2017-01-01

    Los GPCRs de dopamina D2 y de serotonina 5-HT2A constituyen las principales dianas de los antipsicóticos. Tras el hallazgo del heterodímero D2−5-HT2A, varios autores han defendido su implicación en la patología de la esquizofrenia. En esta investigación, se ha estudiado in vitro el cross-talk funcional en el heterocomplejo D2−5-HT2A. Se observó que la expresión y bloqueo del receptor D2 desencadenaron, respectivamente, procesos de cooperatividad positiva y negativa, en la eficacia de activaci...

  17. Fire effects in Pinus uncinata Ram plantations

    Energy Technology Data Exchange (ETDEWEB)

    Cardil Forradellas, A.; Molina Terrén, D.M.; Oliveres, J.; Castellnou, M.

    2016-07-01

    Aim of study: Understanding fire ecology of main forest species is essential for a sound, scientifically based on managing of wildlands and also to assess likely implications due to changes in fire regime under a global change scenario. Few references can be found about fire ecology of Pinus uncinata Ram. (PU). PU species grows in the Central Pyrenees where large, severe wildland fires did not occur frequently in the past. However, several fires with extreme fire behavior have affected PU stands in last years and they might disturb other PU forest in the future. Area of study: Cabdella fire (February 2012), in Lleida province, is one of the several wildland fires occurred in 2012 (winter season) in the Central Pyrenees. Fire affected a large PU plantation (102 ha) located at 1.800-2,100 meters above the sea. Material and methods: We have analyzed first order fire effects in three fireline intensity thresholds along three years in terms of mortality ratio, scorched height, percentage of scorched crown volume and bark char height. Main results: PU seems to be a very tolerant species to low and medium fire line intensity but fire effects were very significant when fire line intensity was high. In medium fireline intensity sites, probability of mortality ranged from 15 to 30% and the dead trees had the highest values on scorched height and percentage of scorched crown volume. Research highlights: Results from this work supports that prescribed burning might be used to efficiently decrease fuel load and fuel vertical continuity while avoiding considerable PU mortality. It also displayed that when fuel management has been implemented, PU mortality might be limited even under extreme fire behavior. (Author)

  18. Effect of the indolacetic acid on the in vitro development of the fruit tissues of the sweet orange var. Salustiana Efecto del ácido indolacético sobre el desarrollo in vitro de los tejidos del fruto de naranjo dulce var. Salustiana

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    Guardiola José

    2007-12-01

    Full Text Available The effect of 4 concentrations of the indolacetic acid as well as the explant type on the in vitro development of the sweet orange fruit tissue [Citrus sinensis (L Osbeck] Salustiana variety was characterized. A maximum response in explants of 6-d-old fruitlets was obtained with the higher concentrations (10-5 and 10-6 M. At this development stage, fruit tissue cells were dividing. A decrease in the fruit response capacity after day 31 was verified. At this moment, cellular differentiation in the internal mesocarp was starting. The increase in size of the vesicle explants of 6-d-old fruitlets suggests a response of the Salustiana cultivar to auxin applications during the early stage of fruit development. This hormone can operate directly on this tissue.

    Se caracterizó el efecto de cuatro concentraciones (10-8 a 10-5 M de ácido indolacético (AIA y del tipo de explanto sobre el desarrollo in vitro de los tejidos del fruto del naranjo dulce Citrus sinensis (L Osbeck, de la variedad salustiana. La máxima respuesta se obtuvo con explantos de frutos de 6 d

  19. Respuesta al glifosato de un aislamiento de Rhizoctonia solani agente causal del anublo de la vaina del arroz, y de cuatro aislamientos de Trichoderma, bajo condiciones in vitro In vitro response of one isolate of Rhizoctonia solani, the pathogen of the rice sheath blight and four isolates of Trichoderma to glyphosate

    Directory of Open Access Journals (Sweden)

    Vargas de Álvarez Amparo

    2002-08-01

    de las malezas, estuviera afectando el crecimiento de R. solani o de su antagonista Trichoderma. Una posible relación entre el uso intensivo del herbicida y la incidencia del añublo de la vaina del arroz, debería investigarse en términos de supervivencia
    y germinación de esclerocios del patógeno.
    Rhizoctonia solani Kuhn is the causal agent ofthe sheath
    blight of rice (Orysa saliva L., one of the most important
    rice diseases in Colombia. G1yphosate (N-phosphonomethyl
    glicine acid is sprayed in rice fields as a preplant herbicide, specially aimed at red rice (O. saliva. Preliminary field observations in Tolima, Colombia suggested a relationship between intensive use of glyphosate and sheath blight incidence. Thus, the present study was undertaken to shed light on the effect of glyphosate on R. solani and Thrichoderma sp., a.putative antagonist, under laboratory conditions. The effect of the herbicide was assessed on the growth ofR. solani in summerged culture and solid media and of Thrichoderma on solid media. R. sotaní withstood up to 300 mg L-! without expressing reduction in growth, Increasing concentrations of the herbicide caused growth reduction, and the detrimental effect reached a plateau at 2500 mg L-1. Rhizoctonia growth was not enhanced at any herbicide concentration. The expected concentration of glyphosate in the soil after spraying at commercial dosages would be O, 75 mg Kg', which is well below the observed in vitro threshold. The response of Thrichoderma was similar to that of R. sotaní. Glyphosate did not affect the antagonistic ability of Thrichoderma against R. solani. These results do not support the view that glyphosate, as an herbicide treatment in rice
    field, may affect in any way the population densities of R.
    sotaní or its antagonist Thrichoderma sp. Therefore, the
    postulated connection between intensive herbicide use
    and increased sheath blight incidence is not substantiated
    on

  20. Chemical modification of coating of Pinus halepensis pollen by ozone exposure.

    Science.gov (United States)

    Naas, Oumsaad; Mendez, Maxence; Quijada, Melesio; Gosselin, Sylvie; Farah, Jinane; Choukri, Ali; Visez, Nicolas

    2016-07-01

    Pollen coating, located on the exine, includes an extractible lipid fraction. The modification of the pollen coating by air pollutants may have implications on the interactions of pollen with plant stigmas and human cells. Pinus halepensis pollen was exposed to ozone in vitro and the pollen coating was extracted with organic solvent and analyzed by GC-MS. Ozone has induced chemical changes in the coating as observed with an increase in dicarboxylic acids, short-chain fatty acids and aldehydes. 4-Hydroxybenzaldehyde was identified as the main reaction product and its formation was shown to occur both on native pollen and on defatted pollen. 4-Hydroxybenzaldehyde is very likely formed via the ozonolysis of acid coumaric-like monomers constitutive of the sporopollenin. Modification of pollen coating by air pollutants should be accounted for in further studies on effect of pollution on germination and on allergenicity. Copyright © 2016 Elsevier Ltd. All rights reserved.

  1. Downregulation of Hepatic De Novo Lipogenesis and Adipogenesis in Adipocytes by Pinus densiflora Bark Extract.

    Science.gov (United States)

    Ahn, Hyemyoung; Jeong, Jeongho; Moyo, Knowledge; Ryu, Yungsun; Min, Bokkee; Yun, Seong Ho; Kim, Hwa Yeon; Kim, Wooki; Go, Gwang-Woong

    2017-11-28

    Korean red pine (Pinus densiflora) bark extract, PineXol (PX), was investigated for its potential antioxidant and anti-inflammation effects in vitro. It was hypothesized that PX treatment (25-150 μg/ml) would reduce the lipid synthesis in HepG2 hepatocytes as well as lipid accumulation in 3T3-L1 adipocytes. Hepatocytes' intracellular triglycerides and cholesterol were decreased in the PX 150 μg/ml treatment group compared with the control (p novo lipogenic proteins (acetyl-CoA carboxylase 1, stearoyl-CoA desaturase 1, elongase of very long chain fatty acids 6, glycerol-3-phosphate acyltransferase 1, and sterol regulatory element-binding protein 1) were significantly decreased in hepatocytes by PX 150 μg/ml treatment compared with the control (p lipogenesis and by inhibiting adipogenesis in adipocytes.

  2. Reference karyotype and cytomolecular map for loblolly pine (Pinus taeda L.)

    Science.gov (United States)

    M. Nurul Islam-faridi; C. Dana Nelson; Thomas L. Kubisiak

    2007-01-01

    A reference karyotype is presented for loblolly pine (Pinus taeda L., subgenus Pinus , section Pinus, subsection Australes), based on fluorescent in situ hybridization (FISH), using 18s-28s rDNA, 5s rDNA, and Arabidopsis-type telomere repeat sequence (A-type TRS). Well...

  3. PRODUÇÃO DE CHAPAS DE MADEIRA COMPENSADA DE CINCO ESPÉCIES DE PINUS TROPICAIS

    Directory of Open Access Journals (Sweden)

    Setsuo Iwakiri

    2001-03-01

    Full Text Available Este trabalho teve como objetivo avaliar o potencial de utilização de 5 espécies de pinus tropicais para produção de painéis compensados. As espécies estudadas foram: Pinus caribaea, Pinus chiapensis, Pinus maximinoi, Pinus oocarpa, Pinus tecunumannii e Pinus taeda, sendo esta última espécie como testemunha. Foram produzidos compensados de 5 lâminas com resinas uréia-formaldeído e fenol-formaldeído. Os resultados de inchamento e recuperação em espessura foram estatisticamente iguais entre as espécies estudadas, com exceção para inchamento em espessura das chapas coladas com resina fenol-formaldeído. As chapas de Pinus maximinoi e Pinus oocarpa, apresentaram melhores resultados de módulos de elasticidade. Para o módulo de ruptura, as chapas de Pinus maxininoi, Pinus oocarpa e Pinus taeda, coladas com resina fenol-formaldeído, apresentaram valores estatisticamente superiores em relação às demais espécies. Quanto a resistência da linha de cola, as chapas de Pinus maximinoi, Pinus taeda e Pinus chiapensis, foram as que apresentaram melhor desempenho. Com base nos resultados gerais da pesquisa, pode-se destacar a potencialidade da madeira de Pinus maximinoi e Pinus oocarpa para produção de chapas de madeira compensada.

  4. Annual Cambial Rhythm in Pinus halepensis and Pinus sylvestris as Indicator for Climate Adaptation.

    Science.gov (United States)

    Prislan, Peter; Gričar, Jožica; de Luis, Martin; Novak, Klemen; Martinez Del Castillo, Edurne; Schmitt, Uwe; Koch, Gerald; Štrus, Jasna; Mrak, Polona; Žnidarič, Magda T; Čufar, Katarina

    2016-01-01

    To understand better the adaptation strategies of intra-annual radial growth in Pinus halepensis and Pinus sylvestris to local environmental conditions, we examined the seasonal rhythm of cambial activity and cell differentiation at tissue and cellular levels. Two contrasting sites differing in temperature and amount of precipitation were selected for each species, one typical for their growth and the other represented border climatic conditions, where the two species coexisted. Mature P. halepensis trees from Mediterranean (Spain) and sub-Mediterranean (Slovenia) sites, and P. sylvestris from sub-Mediterranean (Slovenia) and temperate (Slovenia) sites were selected. Repeated sampling was performed throughout the year and samples were prepared for examination with light and transmission electron microscopes. We hypothesized that cambial rhythm in trees growing at the sub-Mediterranean site where the two species co-exist will be similar as at typical sites for their growth. Cambium in P. halepensis at the Mediterranean site was active throughout the year and was never truly dormant, whereas at the sub-Mediterranean site it appeared to be dormant during the winter months. In contrast, cambium in P. sylvestris was clearly dormant at both sub-Mediterranean and temperate sites, although the dormant period seemed to be significantly longer at the temperate site. Thus, the hypothesis was only partly confirmed. Different cambial and cell differentiation rhythms of the two species at the site where both species co-exist and typical sites for their growth indicate their high but different adaptation strategies in terms of adjustment of radial growth to environmental heterogeneity, crucial for long-term tree performance and survival.

  5. Evaluación de tres métodos para el arrastre de madera en rodales naturales de Pinus caribaea var. caribaea Evaluation of three methods of the log Pinus caribaea skidding in natural set of wheels

    Directory of Open Access Journals (Sweden)

    Fidel Cándano Acosta

    2004-06-01

    Full Text Available El trabajo se realizó en rodales naturales de Pinus caribaea var. caribaea, ubicados en la unidad silvícola San Andrés, La Palma, provincia Pinar del Río. El objetivo de la investigación fue evaluar la eficiencia y los daños causados a la cubierta vegetal del suelo durante la operación de arrastre de madera. Se evaluaron tres métodos: 1 - el arrastre de fustes enteros con yuntas de bueyes hasta los patios de carga; 2 - el arrastre de fustes enteros con tractor hasta los patios de carga; 3 - el arrastre de fustes enteros con yuntas de bueyes hasta las pistas de arrastre y con tractor hasta los patios de carga. El método de arrastre de madera que consistió en la combinación del empleo de animales y de tractor resultó más eficiente y provocó menos alteraciones a la cubierta vegetal del suelo.The work was carried out in natural stand of Pinus caribaea var. caribaea located in San Andrés, Forestry enterprise of La Palma in the Pinar del Río province. The objective was determine which method of wood extraction results more efficient and reduce damages to the soil vegetable. Three method were evaluated: 1- the extraction of tree length with oxen to landing; 2 - the extraction of tree length with tractor to landing; 3 - the extraction of tree length with oxen to short distance and using tractor up to landing places. The method the extraction of tree length with oxen to short distance and using tractor up to landing places, resulted the most efficient and the one which fewer damages on the vegetable cover of the soil.

  6. CARACTERIZACIÓN Y EVALUACIÓN in vivo E in vitro DEL LIPOPOLISACÁRIDO DE Aeromonas hydrophila In Vitro/In Vivo Characterization And Evaluation Of Aeromonas hydrophila Lipopolysacharides (LPS

    Directory of Open Access Journals (Sweden)

    AP JIMÉNEZ

    Full Text Available A partir de una cepa de A. hydrophila aislada de un brote de enfermedad septicémica en Tilapia nilótica (Piaractus brachypomusoreochromis niloticus, se obtuvieron extractos de lipopolisacárido (LPS crudo (29,5 mg/ml y semipurificado (106,5 mg/ml mediante la técnica fenol-agua caliente descrita por Westphal, Jann (1965. La presencia de proteína fue del 2,3% para el extracto crudo y de 0,1% para el semipurificado; la concentración de polisacáridos osciló entre el 15 y 26%. En electroforesis (SDS-PAGE se observaron bandas de 14 Kd correspondientes al oligosacárido central y al lípido A del LPS. Tres ratones de 25-35 g fueron inoculados intraperitonealmente con 25 mg/Kg de LPS cru-do, a partir de la primera hora todos los animales mostraron erizamiento, taquipnea e inapetencia; microscópicamente se detectó congestión hepática y pulmonar, hemorragias pulmonares y renales, marginación leucocitaria en hígado y pulmón con predominio de polimorfo-nucleares neutrófilos (PMN en todos los animales, mostrando un mayor efecto que el control inoculado con LPS de E. coli (Sigma® a la misma concentración. In vitro el LPS crudo a concentración de 10, 20 y 30 µg/ml indujo proliferación de células mono-nucleares murinas (2 x 10 5 en 200 µl de medio DMEM por incorporación de timidina tritiada; tanto el LPS control (E. coli, como el LPS crudo de A. hydrophila mostraron cuentas por minuto (CPM ascendentes de manera dosis dependiente, el LPS de A. hydrophila desencadeno una proliferación muy similar a la inducida por el control.Aeromonas hydrophila isolated from a septicemic disease outbreak in tilapia (Piaractus brachypomusoreochromis niloticus was used to prepare crude (29.5 mg/ml and semipurified (106.5 mg/ml lipopolysacharide extracts (LPS based on the phenol-hot water protocol (Westphal and Jann, 1965. Protein contents were 2.3% and 0.1% for the crude and the semipurified extracts, respectively, while the polysacharides ranged from 15 to

  7. Estudio de potenciales alelopáticos originados por Eucalyptus globulus Labill., Pinus pinaster Ait. y Pinus radiata D.

    OpenAIRE

    Ballester, A.; Arias, A. M.; Cobián, B.; López Calvo, E.; Vieitez, E.

    2011-01-01

    Se ha estudiado el potencial alelopático de Eucalyptus globulus Labill., Pinus pinaster Ait. y Pinus radiata D. sobre el crecimiento y la germinación de diferentes especies herbáceas. Extractos acuosos de hojas y acículas recogidas en los meses de enero y abril inhiben fundamentalmente la germinación de las semillas de festuca, siendo la acción más importante en el mes de abril que en el de enero. El contacto directo entre hojas y acículas y las semillas a ensayar produce una inhibición muy f...

  8. EFECTO DE LOS INCENDIOS FORESTALES EN LA REGENERACIÓN DE Pinus oocarpa var. ochoterenae

    Directory of Open Access Journals (Sweden)

    A. Juárez-Martínez

    2003-01-01

    Full Text Available Los incendios forestales favorecen la regeneración natural de muchos pinos. Por ello se estudió el renuevo de Pinus oocarpa var. ochoterenae en el municipio Sola de Vega, Oaxaca, a dos y cuatro años de haber acontecido incendios forestales, usando una aproximación sincrónica. En cada caso fueron considerados tres niveles de intensidad del fuego superficial (en términos relativos: alta, mediana y baja y un testigo. Para el análisis de datos se utilizó un procedimiento mixto, con un modelo lineal condicional jerárquico. Se halló que en las áreas incendiadas hubo más regeneración que en las no incendiadas, que tanto a dos como a cuatro años de los incendios, la densidad de brinzales fue mayor en los sitios afectados a alta intensidad, que el pulso de la regeneración aconteció a dos años del fuego en ambos casos, y que la regeneración tiende a reducir su densidad a través del tiempo.

  9. Influencia de la edad de corte del pasto morado (Pennisetum purpureum en la producción y digestibilidad in vitro de la materia seca

    Directory of Open Access Journals (Sweden)

    N. B. Madera

    2013-01-01

    Full Text Available En el presente trabajo se evaluaron diferentes edades de corte del pasto morado (Pennisetum purpureum, para conocer su influencia en la producción y digestibilidad in vitro de la materia seca (DIVMS. El estudio se realizó de diciembre de 2008 a junio de 2009, considerándose desde el establecimiento hasta la evaluación de los tratamientos (edades de corte de 45, 60, 75, 90, 105 y 120 días, distribuidos en un diseño de bloques completos al azar y cuatro repeticiones. Se encontró diferencia (P≤0.001 entre las edades de corte en todas las variables evaluadas, excepto para el número de hijuelos (P>0.05. Se observó que con el aumento de la edad de corte, se incrementa la altura y circunferencia de la cepa, largo y ancho de la hoja, diámetro basal del entrenudo y producción de forraje. En la relación hoja/tallo se encontró una disminución, al aumentar la edad de corte (P≤0.001. A medida que se incrementa la edad de corte, la DIVMS disminuye linealmente, obteniéndose R2 de 90.63, 94.95, 93.65 y ecuaciones de regresión: Y = 74.5933 - 0.221313x, Y = 80.399 - 0.413524x, Y = 74.3048 - 0.292381x para hoja, tallo y planta completa, respectivamente. Se concluye que con el corte entre los 75-90 días, el pasto morado logra cepas de mayor altura y circunferencia, con hojas superiores en diámetro y longitud; a la vez que incrementa la producción de biomasa. A diferencia de la relación hoja/tallo y la DIVMS, que disminuyen conforme se incrementa la edad de corte.

  10. Susceptibilidad in vitro a los medicamentos anti-tuberculosos de aislados de cepas del complejo Mycobacterium tuberculosis obtenidos a partir de lobos marinos

    Directory of Open Access Journals (Sweden)

    Amelia Bernardelli

    2004-06-01

    Full Text Available Se han hallado cepas de micobacterias aisladas de lobos marinos del Atlántico sur y pertenecen al complejo de Mycobacterium tuberculosis. Los animales se recibieron en las instalaciones del Oceanario Mundo Marino y fueron tratados apropiadamente para su recuperación con la terapia convencional, cuidados intensivos y suplemento alimentario pero no se observó mejoría en su estado general. Se practicaron necropsias en todos los animales y se observaron lesiones extensas compatibles con tuberculosis en pulmones, hígado, bazo y ganglios linfáticos. Para la identificación de las micobacterias, se realizaron pruebas bioquímicas y técnicas de biología molecular con la sonda IS6110. Además, se identificaron todas las cepas como pertenecientes al complejo M. tuberculosis mediante el equipo LCx M. tuberculosis Assay (Abbott Laboratories. El objetivo de este estudio fue determinar in vitro la sensibilidad de las cepas patrón BCG, H37Rv (M. tuberculosis y AN5 (Mycobacterium bovis y la de las siete aisladas de lobos marinos a isoniacida, rifampicina, estreptomicina y etambutol. La concentración inhibitoria mínima (CIM de las drogas antituberculosas se llevó a cabo con el equipo Mycobacterial Growth Indicator Tube (MGIT, BD, Argentina y la microdilución con el ensayo colorimétrico con bromuro de 3-(4-5 dimetiltiazol-2-2,5 difeniltetrazolio. Todos los aislamientos y las cepas de referencia BCG y AN5 se inhibieron con valores CIM de los de H37Rv con buena concordancia entre los resultados obtenidos con ambas técnicas. Los hallazgos permiten sugerir que podrían ser una importante ayuda terapéutica en los lobos marinos con diagnóstico de tuberculosis y evaluar el posible papel sanitario en la prevención y transmisión de la tuberculosis de los animales a los humanos y el trabajo en conjunto.

  11. Growth process and diameter structure of Pinus tabulaeformis forest ...

    African Journals Online (AJOL)

    Using stem analysis method, the biomass, growing process and diameter structure of 21-year shady and sunny slope Pinus tabulaeformis forest were investigated in hilly loess-gully region. Results showed that there were distinct difference in the indexes, tree height, diameter at breast height (DBH) and timber volume ...

  12. Evaluation of genetic diversity of Portuguese Pinus sylvestris L ...

    Indian Academy of Sciences (India)

    Home; Journals; Journal of Genetics; Volume 92; Online resources. Evaluation of genetic diversity of Portuguese Pinus sylvestris L. populations based on molecular data and inferences about the future use of this germplasm. J. Cipriano A. Carvalho C. Fernandes M. J. Gaspar J. Pires J. Bento L. Roxo J. Louzada J. Lima- ...

  13. Evaluation of the Leave and Bud Decoctions Pinus Halepensis Mill ...

    African Journals Online (AJOL)

    Pinus halepensis M. (Pinaceae) has done object many indications in traditional medicine. Several studies have demonstrated a protective activity of essential oils extracted from various plant species against renal toxicity induced by chemicals in the animal model. The objective of this study is to assess the anti toxic effects ...

  14. Missing and dark rings associated with drought in Pinus halepensis

    Science.gov (United States)

    Klemen Novak; Martin De Luis; Jozica Gricar; Peter Prislan; Maks Merela; Kevin T. Smith; Katarina. Cufar

    2016-01-01

    The responses of the vascular cambium and tracheid differentiation to extreme drought in Aleppo pine (Pinus halepensis Mill.) were investigated. The research focused on the drought year of 2005, in the primary study area at Maigmo (MAI) in southeastern Spain, with comparisons in Jarafuel (JAL) and Guardamar (GUA). The climate in this region is...

  15. Determination of processed soybean meal degradability by Pinus ...

    African Journals Online (AJOL)

    Protected soybean meal is an important part of high producing dairy cow diet and many methods are used for its safe and economic processing. Pinus eldarica contains xylose and resins and results show that these components could affect dry matter and crude protein degradability. This is the first time a study is being ...

  16. Evaluation of seed production of scots pine ( Pinus sylvestris L ...

    African Journals Online (AJOL)

    This research was carried out to investigate seed production in a 13 years-old scots pine (Pinus sylvestris L.) clonal seed orchard, including 30 clones. Eight of cone and seed traits as number of fertile and infertile scales, cone volume, cone number, filled and empty seed number, seed efficiency and 1000 seed weight were ...

  17. Genomic DNA extraction from sapwood of Pinus roxburghii for ...

    African Journals Online (AJOL)

    Ashish

    2013-02-22

    Feb 22, 2013 ... A method for extraction of genomic DNA from sapwood tissues of mature tall trees of Pinus roxburghii, where collection of needle tissues is extremely difficult has been standardized. The extracted DNA was comparable to that obtained from the needle tissue in terms of yield and purity. The yield of extracted.

  18. The effects of fepeated prescribed burning on Pinus ponderosa growth

    Science.gov (United States)

    David L. Peterson; Stephen S. Sackett; Lindsay J. Robinson; Sally M. Haase

    1994-01-01

    The effect of repeated prescribed burning on long term growth of Pinus ponderosa in northern Arizona was examined. Fire treatments for hazard reduction were initiated in 1976,and growthwas evaluated in 1988 for fire rotations of 1, 2, 4, 6, 8, and 10 years. Dendroecological analysis shows that there were only small changes in treegrowth (compared tocontrols) in the...

  19. An interesting chemical polymorphism in Pinus sylvestris L.

    Directory of Open Access Journals (Sweden)

    Jerzy Szweykowski

    2014-01-01

    Full Text Available Intra- and interpopulational polymorphism in the production of phenolic compounds is described in Polish populations of Pinus sylvestris L. Two mutually exclusive forms of pine trees are present in changing proportions in all populations studied. This allows three groups of populations to be distinguished. The character of this differentiation is discussed.

  20. Drying schedules calculation of Camiyani Black Pine ( Pinus nigra ...

    African Journals Online (AJOL)

    In this study, computer aided drying schedules were developed for Camiyani Black Pine (Pinus nigra var. pallasiana) lumber for less than 30 mm thick, between 30-60 mm thick and larger than 60 mm. Schedules were calculated on drying gradient basis. In this software (named KILNBRAIN), users can find more then one ...

  1. Analgesic and Anti-Inflammatory Activity of Pinus roxburghii Sarg.

    Directory of Open Access Journals (Sweden)

    Dhirender Kaushik

    2012-01-01

    Full Text Available The Chir Pine, Pinus roxburghii, named after William Roxburgh, is a pine native to the Himalaya. Pinus roxburghii Sarg. (Pinaceae is traditionally used for several medicinal purposes in India. As the oil of the plant is extensively used in number of herbal preparation for curing inflammatory disorders, the present study was undertaken to assess analgesic and anti-inflammatory activities of its bark extract. Dried and crushed leaves of Pinus roxburghii Sarg. were defatted with petroleum ether and then extracted with alcohol. The alcoholic extract at the doses of 100 mg/kg, 300 mg/kg, and 500 mg/kg body weight was subjected to evaluation of analgesic and anti-inflammatory activities in experimental animal models. Analgesic activity was evaluated by acetic acid-induced writhing and tail immersion tests in Swiss albino mice; acute and chronic anti-inflammatory activity was evaluated by carrageenan-induced paw oedema and cotton pellet granuloma in Wistar albino rats. Diclofenac sodium and indomethacin were employed as reference drugs for analgesic and anti-inflammatory studies, respectively. In the present study, the alcoholic bark extract of Pinus roxburghii Sarg. demonstrated significant analgesic and anti-inflammatory activities in the tested models.

  2. Evaluation of genetic diversity of Portuguese Pinus sylvestris L ...

    Indian Academy of Sciences (India)

    Okhotsk Sea in eastern Siberia, and from beyond the sub- arctic forests of northern Scandinavia to arid, mountain- ous southern areas of Spain (Labra et al. 2006; Pyhäjärvi et al. ... in light of global climatic changes. Keywords. genetic diversity; ISSR; RAPD; Pinus sylvestris L. Journal of Genetics Vol. 92, Online Resources.

  3. Percentile-based Weibull diameter distribution model for Pinus ...

    African Journals Online (AJOL)

    This study was conducted to develop a diameter distribution model for Pinus kesiya stands in Benguet province, Philippines, using the percentile-based Weibull model. The performance of the different equations was examined using evaluation statistics such as the fit index (FI) and root mean square error (RMSE). Results ...

  4. evaluation of the leave and bud decoctions pinus halepensis mill ...

    African Journals Online (AJOL)

    31 déc. 2014 ... ABSTRACT. Pinus halepensis M. (Pinaceae) has done object many indications in traditional medicine. Several studies have demonstrated a protective activity of essential oils extracted from various plant species against renal toxicity induced by chemicals in the animal model. The objective of this study is ...

  5. Characteristics, histories, and future succession of northern Pinus pugens stands

    Science.gov (United States)

    Patrick Brose

    2017-01-01

    Pinus pungens (Table Mountain pine) stands are rare conifer-dominated communities that occur on xeric ridges and upper slopes throughout the central and southern Appalachian Mountains. At the northern end of this range, this uncommon forest community is essentially unstudied. Therefore, in 2006 I initiated a dendroecology study of three ...

  6. Recovery of Collembola in Pinus tabulaeformis plantations

    Science.gov (United States)

    Liang CHANG; Baifeng WANG; Xianghui LIU; Mac A. CALLAHAM; Feng GE

    2017-01-01

    Large areas of forest plantations have been developed in China. It is important to evaluate the soil fauna in plantations and the conditions needed for their recovery in view of the large areas of plantations in China. Three Pinus tabulaeformis forests, a 26-year-old plantation (P26) and a 45-year-old plantation (P45), exposed to clear-...

  7. Some physical and strength properties of immature Pinus patula ...

    African Journals Online (AJOL)

    A study was conducted to determine physical and strength properties of immature Pinus patula grown in Iringa and Njombe regions of Tanzania. Sample trees aged 5 to 15 years were collected from farmers' woodlots. The trees were categorized into 5 age classes: 5 - 7, 8 - 10, 11 - 12, 13 - 14 and 15 years. Four trees from ...

  8. Establishment of embryogenic suspension cultures of Pinus radiata ...

    African Journals Online (AJOL)

    The development of embryonal suspensor mass (ESM) from immature embryos of Pinus radiata on a solidified growth medium containing 0, 5 mgl -1 benzyladenine, 3, 0 mgl -1 2, 4-dichlorophenoxyacetic acid, 500 mgl -1 casein hydrolysate and 250 mgl -1 L-glutamine was used as inoculum to establish cell suspension ...

  9. Antifungal metabolites from fungal endophytes of Pinus strobus

    DEFF Research Database (Denmark)

    Sumarah, Mark W; Kesting, Julie R; Sørensen, Dan

    2011-01-01

    The extracts of five foliar fungal endophytes isolated from Pinus strobus (eastern white pine) that showed antifungal activity in disc diffusion assays were selected for further study. From these strains, the aliphatic polyketide compound 1 and three related sesquiterpenes 2-4 were isolated...

  10. Relative size and stand age determine Pinus banksiana mortality

    Science.gov (United States)

    Han Y. H. Chen; Songling Fu; Robert A. Monserud; Ian C. Gillies

    2008-01-01

    Tree mortality is a poorly understood process in the boreal forest. Whereas large disturbances reset succession by killing all or most trees, background tree mortality was hypothesized to be affected by competition, ageing, and stand composition. We tested these hypotheses on jack pine (Pinus banksiana Lamb.) mortality using data from long-term...

  11. Effects of Arceuthobium americanum on twig growth of Pinus contorts.

    Science.gov (United States)

    Nancy Broshot; Lynn Larsen; Robert. Tinnin

    1986-01-01

    Patterns of branch growth in Pinus contorta Dougl. ex Loud, (lodgepole pine) on the east side of the Cascade Range in Oregon were significantly altered by Arceuthobium americanum (lodgepole pine dwarf mistletoe). There were decreases in the number, length, and mass of needles, as well as in the length and mass of twigs. These...

  12. Forest health and vitality: the detection and monitoring of Pinus ...

    African Journals Online (AJOL)

    Forest health and vitality: the detection and monitoring of Pinus patula trees infected by Sirex noctilio using digital multispectral imagery. ... Southern Forests: a Journal of Forest Science ... Broad-scale visual assessments of infestation provided by forest managers are currently used to measure forest health and vitality.

  13. Evolution of genome size and complexity in Pinus.

    Directory of Open Access Journals (Sweden)

    Alison M Morse

    Full Text Available BACKGROUND: Genome evolution in the gymnosperm lineage of seed plants has given rise to many of the most complex and largest plant genomes, however the elements involved are poorly understood. METHODOLOGY/PRINCIPAL FINDINGS: Gymny is a previously undescribed retrotransposon family in Pinus that is related to Athila elements in Arabidopsis. Gymny elements are dispersed throughout the modern Pinus genome and occupy a physical space at least the size of the Arabidopsis thaliana genome. In contrast to previously described retroelements in Pinus, the Gymny family was amplified or introduced after the divergence of pine and spruce (Picea. If retrotransposon expansions are responsible for genome size differences within the Pinaceae, as they are in angiosperms, then they have yet to be identified. In contrast, molecular divergence of Gymny retrotransposons together with other families of retrotransposons can account for the large genome complexity of pines along with protein-coding genic DNA, as revealed by massively parallel DNA sequence analysis of Cot fractionated genomic DNA. CONCLUSIONS/SIGNIFICANCE: Most of the enormous genome complexity of pines can be explained by divergence of retrotransposons, however the elements responsible for genome size variation are yet to be identified. Genomic resources for Pinus including those reported here should assist in further defining whether and how the roles of retrotransposons differ in the evolution of angiosperm and gymnosperm genomes.

  14. Evolutionary relationships of Slash Pine ( Pinus elliottii ) with its ...

    African Journals Online (AJOL)

    llozymes in bud tissue and monoterpene contents in xylem oleoresin of slash pine (Pinus elliottii) were analyzed from populations across the natural distribution, as well as those from other species in the AUSTRALES pines. Allozyme diversity measures of slash pine were similar to those found in other southern pines.

  15. Tissue culture in Pinus caribaea Mor. var. Hondurensis barr. and ...

    African Journals Online (AJOL)

    Tissue culture in Pinus caribaea Mor. var. Hondurensis barr. ... However, different degrees of greening were observed in some of the cultures (both compact and friable type). Thus ... Anatomical studies indicated that the differences between the compact and friable calluses were in the distribution of the meristematic cells.

  16. Aboveground tree biomass for Pinus ponderosa in northeastern California

    Science.gov (United States)

    Martin W. Ritchie; Jianwei Zhang; Todd A. Hamilton

    2013-01-01

    Forest managers need accurate biomass equations to plan thinning for fuel reduction or energy production. Estimates of carbon sequestration also rely upon such equations. The current allometric equations for ponderosa pine (Pinus ponderosa) commonly employed for California forests were developed elsewhere, and are often applied without consideration potential for...

  17. Growth process and diameter structure of Pinus tabulaeformis forest ...

    African Journals Online (AJOL)

    STORAGESEVER

    2009-10-19

    Oct 19, 2009 ... Using stem analysis method, the biomass, growing process and diameter structure of 21-year shady and sunny slope Pinus tabulaeformis forest were investigated in hilly loess-gully region. Results showed that there were distinct difference in the indexes, tree height, diameter at breast height (DBH).

  18. Charcoal production from Pinus species in the laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Brito, J.O.; Barrichelo, L.E.G.; Pontinha, A.A.S.

    1978-01-01

    Charcoal produced from wood of Pinus strobus var. chiapensis (13 years old) and P. elliottii var. elliotti (15 years old) was compared with charcoal from Eucalyptus maculata and E. camaldulensis (both 4-5 year old). Although their charcoal was inferior to E. maculata charcoal in several respects, both pine species produced better charcoal than E. camaldulensis.

  19. Growth and provenance variation of Pinus caribaea var ...

    African Journals Online (AJOL)

    A total of 21 provenances and sources of Pinus caribaea var. hondurensis were tested in 48 provenance / progeny trials in Brazil, South Africa, and Venezuela. Growth rates in Brazil and Venezuela were quite promising, and were less encouraging in Colombia. In Brazil and Venezuela, heights were around 12 m and mean ...

  20. Genomic DNA extraction from sapwood of Pinus roxburghii for ...

    African Journals Online (AJOL)

    A method for extraction of genomic DNA from sapwood tissues of mature tall trees of Pinus roxburghii, where collection of needle tissues is extremely difficult has been standardized. The extracted DNA was comparable to that obtained from the needle tissue in terms of yield and purity. The yield of extracted DNA ranged ...

  1. Produção de chapas de madeira compensada de cinco espécies de pinus tropicais

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    Setsuo Iwakiri

    2001-01-01

    Full Text Available This research was developed aiming at evaluating the feasibility of the use of 5 species of tropical pine to plywood manufacture. The following species were studied: Pinus caribaea, Pinus chiapensis, Pinus maximinoi, Pinus oocarpa, Pinus tecunumannii and Pinus taeda, being the last used as the referential species. Plywood were manufactured with 5 plies, bonded with ureaformaldheyde and fenol-formaldheyde resin. The results of thickness sweeling and recovering were the same for all species studied, with exception to thickness sweeling for the boards glued with fenolformaldheyde resin. The boards made from Pinus maximinoi and Pinus oocarpa, showed the higher values in modulus of elasticity. The boards of Pinus maximinoi, Pinus oocarpa and Pinus taeda, glued with fenol-formaldheyde resin, resulted in higher values of the modulus of rupture, in comparison to other species. For the glue line strength, the boards of Pinus maximinoi, Pinus taeda and Pinus chiapensis, showed the better results. Based on the general results of this research it, could be said that the Pinus maximinoi and Pinus oocarpa present the high potentiality to plywood manufacture.

  2. PRODUÇÃO DE CHAPAS DE MADEIRA COMPENSADA DE CINCO ESPÉCIES DE PINUS TROPICAIS

    OpenAIRE

    Setsuo Iwakiri; Danielle Previdi Olandoski; Gabriela Leonhardt; Martha Andreia Brand

    2001-01-01

    Este trabalho teve como objetivo avaliar o potencial de utilização de 5 espécies de pinus tropicais para produção de painéis compensados. As espécies estudadas foram: Pinus caribaea, Pinus chiapensis, Pinus maximinoi, Pinus oocarpa, Pinus tecunumannii e Pinus taeda, sendo esta última espécie como testemunha. Foram produzidos compensados de 5 lâminas com resinas uréia-formaldeído e fenol-formaldeído. Os resultados de inchamento e recuperação em espessura foram estatisticamente iguais entre as ...

  3. Efeitos neuromusculares in vitro e in vivo do atracúrio e do rocurônio em ratos submetidos a tratamento de sete dias com carbamazepina Efectos neuromusculares in vitro e in vivo del atracurio y del rocuronio en ratones sometidos a tratamiento de siete días con carbamazepina In vitro and in vivo neuromuscular effects of atracurium and rocuronium in rats treated with carbamazepine for seven days

    Directory of Open Access Journals (Sweden)

    Caroline Coutinho de Barcelos

    2008-04-01

    neuromuscular producido por el rocuronio y atracurio en ratones tratados con carbamazepina y determinó las concentraciones de citocromo P450 y b5 reductasis en microsomas hepáticos. MÉTODO: Ratones fueron tratados por siete días con carbamazepina (CBZ - 40 mg.kg-1 a través de una sonda y sacrificados al octavo día bajo anestesia con uretana. Las preparaciones in vitro e in vivo fueron montadas de acuerdo con las técnicas de Bulbring y de Leeuwin y Wolters, respectivamente. Las concentraciones y dosis utilizadas de los bloqueadores en las preparaciones in vitro e in vivo fueron, respectivamente, 20 µg.mL-1 y 0,5 mg.kg-1 para atracurio (ATC; 4 µg.mL-1 y 0,6 mg.kg-1 para rocuronio (ROC. Cada protocolo tuvo un n = 5 y las respuestas fueron observadas por 60 minutos. Los efectos del ATC y ROC fueron evaluados en las preparaciones de ratones tratados (Cbz t y comparados a los observados en los de ratones no tratados (CBZst. Las concentraciones de citocromo P450 y b5 reductasis fueron determinadas en microsomas aislados de hígados de ratones tratados (CBZt y comparadas con las obtenidas en ratones no tratados (CBZst RESULTADOS: La carbamazepina no alteró la amplitud de las respuestas musculares; in vitro y in vivo, no hubo diferencia entre el bloqueo neuromuscular producido por el atracurio en las preparaciones CBZt versus CBZst; el bloqueo neuromuscular producido por el Rocuronio en las preparaciones CBZt fue potenciado in vitro. La carbamazepina no alteró las concentraciones de citocromo P450 y b5. CONCLUSIONES: El tratamiento por siete días con carbamazepina, no influenció en el bloqueo producido por el atracurio, y alteró in vitro los efectos del rocuronio. El tiempo de tratamiento no fue suficiente para causar la inducción enzimática y disminuir la sensibilidad al rocuronio.BACKGROUND AND OBJECTIVES: This experimental study investigated the in vitro and in vivo neuromuscular blockade of rocuronium and atracurium in rats treated with carbamazepine and determined

  4. OBTENCIÓN IN VITRO Y CARACTERIZACIÓN DE CÉLULAS STEM DEL CORDÓN UMBILICAL HUMANO COMO ALTERNATIVA DE LAS CÉLULAS STEM DE ORIGEN EMBRIONARIO PARA LA MEDICINA REGENERATIVA

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    Juan Carlos Munévar Niño

    2005-01-01

    Full Text Available Durante siglos el hombre ha tratado de comprender la capacidad del cuerpo para reparar y reemplazar las células y tejidos del organismo. Después de años de trabajo dilucidando el como y el por qué de los mecanismos de reparación y regeneración tisular, los científicos se han enfocado en las células Stem. La identificación y aislamiento de células Stem de numerosos tejidos embrionarios y posnatales provee objetivos apropiados para una variedad de prácticas biotecnológicas denominadas generalmente como Medicina Regenerativa e Ingeniería Tisular. Desde el descubrimiento sobre la capacidad de las células Stem adultas para formar diferentes tipos de tejidos in vivo e in vitro, como una fuente alternativa para las células Stem embrionarias, lo que ofrece amplios potenciales terapéuticos para los seres humanos. La obtención de éstas células a partir del cordón umbilical humano es un sustituto interesante porque es un órgano fetal, fácil de obtener, descartable, lo que disminuye las dificultades bioéticas. En la Universidad El Bosque estamos aislando y caracterizando in vitro células Stem mesenquimatosas de la gelatina de Wharton del cordón umbilical de neonatos, obtenido previo consentimiento informado. Este sistema permitió obtener células precursoras viables de rápida proliferación que expresaron patrones de marcaje FGFR 3 (+, abriendo la puerta para poder diferenciarlas in vitro con fines terapéuticos.

  5. Impact Of Pine (Pinus sylvestris L. And Spruce (Picea abies (L. Karst. Bark Extracts On Important Strawberry Pathogens

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    Minova Sandra

    2015-04-01

    Full Text Available Phytopathogenic fungi induced considerable economic losses in strawberry production industry; therefore, more attention should be paid to development and implementation of preventative treatment that is environmentally friendly. Coniferous trees produce a wide variety of compounds, such as terpenoids and phenolics. Several studies are known on fungicidal activity of different components of coniferous tree bark. The aim of this study was to evaluate in vitro pine (Pinus sylvestris L. and spruce (Picea abies (L. Karst. bark ethanol extracts impact on pathogenous fungi causing diseases of strawberries. Products of processed pine (Pinus sylvestris and spruce (Picea abies bark were tested. During 2011 to 2013, several in vitro experiments were carried out to test the effectiveness of pine and spruce bark extracts against various phytopathogenic fungi isolated from strawberries: Botrytis cinerea, Colletotrichum acutatum, Phytophthora cactorum and Mycosphaerella fragariae. Radial growth tests showed that coniferous bark extracts inhibit mycelial growth of B. cinerea, C. acutatum, P. cactorum and M. fragariae. Extracts had the highest antifungal effect on B. cinerea two and five days after inoculation (p < 0.05. Bark extracts can reduce the sporulation of B. cinerea, C. acutatum and P. cactorum.

  6. ECTOMICORRIZAS ASOCIADAS A Pinus jeffreyi EN EL PARQUE NACIONAL "CONSTITUCIÓN DE 1857" EN BAJA CALIFORNIA, MÉXICO

    Directory of Open Access Journals (Sweden)

    Selene Aguilar-Aguilar

    2011-01-01

    Full Text Available Se estudió la diversidad de hongos presentes en tres áreas boscosas del Parque Nacional Constitución de 1857 en Baja California, México. Las colectas se realizaron en agosto de 2007 y febrero, mayo y agosto de 2008, obteniendo 25 ejemplares correspondientes a tres géneros: Geastrum (cuatro especies, Suillus (una especie y Laccaria (una especie. La abundancia de especies estuvo relacionada con la precipitación y condiciones ecológicas propias del sitio. Se realizaron muestreos para colectar raíces secundarias de árboles adultos en Pinus jeffreyi Grev. & Balf y se identificaron los morfotipos ectomicorrícicos usando las claves DEMMY. Se encontraron seis morfotipos diferentes, lo que sugiere que existe asociación ectomicorrícica de P. jeffreyi con al menos seis diferentes especies de hongos presentes en esta área.

  7. Protetores físicos para semeadura direta de Pinus elliottii Engelm Shelters for direct seeding of Pinus elliottii Engelm

    Directory of Open Access Journals (Sweden)

    Vilmar Luciano Matteiq

    2001-10-01

    Full Text Available A utilização de protetores físicos tem se revelado uma eficiente técnica para o sucesso da semeadura direta de pinus. O presente trabalho avaliou diferentes tipos e tamanhos de protetores físicos de pontos de semeadura para a implantação de povoamentos de Pinus elliottii por semeadura direta. Como protetores, foram utilizados copos plástico, copos de papel e laminado de madeira. Esses protetores foram colocados sobre os pontos semeados com três sementes cada. Foi avaliada a emergência, sobrevivência, perdas por pássaros, perdas por tombamento e número de pontos com, pelo menos, uma planta viva, seis meses após a semeadura. Os protetores utilizados influenciaram positivamente, nos resultados, para todas as variáveis analisadas, por evitar perdas de sementes causadas por arraste ou soterramento e, principalmente por diminuíram os danos causados por pássaros que juntamente com as formigas, foram os principais problemas encontrados na implantação do povoamento de pinus por semeadura direta.The use of individual shelters has become an efficient method for direct seeding of pine. This work investigated the use of different kinds and sizes of shelters placed at sowing sites to establish new populations of directly seeded Pinus elliottii. Plastic and paper cups with open bottoms, or wood slats were used as protective shields. These were placed at sowing sites, each one containing 3 seeds. Emergencies, survival, losses due to birds or lodging, and number of sites with at least one live plant were analyzed six months after sowing. Shelters had positive effects on all variables analyzed both for avoiding losses resulted from dragging or burying of seeds, and for diminidhing the harm caused by birds which along with ants were the main problems found during the establishment of directly seeded pinus population.

  8. Terpene chemodiversity of relict conifers Picea omorika, Pinus heldreichii, and Pinus peuce, endemic to Balkan.

    Science.gov (United States)

    Nikolić, Biljana; Ristić, Mihailo; Tešević, Vele; Marin, Petar D; Bojović, Srdjan

    2011-12-01

    Terpenes are often used as ecological and chemotaxonomic markers of plant species, as well as for estimation of geographic variability. Essential oils of relic and Balkan endemic/subendemic conifers, Picea omorika, Pinus heldreichii, and P. peuce, in central part of Balkan Peninsula (Serbia and Montenegro), on the level of terpene classes and common terpene compounds were investigated. In finding terpene combinations, which could show the best diversity between species and their natural populations, several statistical methods were applied. Apart from the content of different terpene classes (P. omorika has the most abundant O-containing monoterpenes and sesquiterpenes; P. heldreichii and P. peuce have the largest abundance of sesquiterpene and monoterpene hydrocarbons, resp.), the species are clearly separated according to terpene profile with 22 common compounds. But, divergences in their populations were established only in combination of several compounds (specific for each species), and they were found to be the results of geomorphologic, climatic, and genetic factors. We found similarities between investigated species and some taxa from literature with respect to terpene composition, possibly due to hybridization and phylogenetic relations. Obtained results are also important regarding to chemotaxonomy, biogeography, phylogeny, and evolution of these taxa. Copyright © 2011 Verlag Helvetica Chimica Acta AG, Zürich.

  9. Determinación de vitamina (a -Tocoferol en Pinus Caribaea Morelet y Eucalyptus SP.

    Directory of Open Access Journals (Sweden)

    Clara Bécquer Romagosa

    1996-08-01

    Full Text Available Se presenta un método rápido de cromatografía de capa delgada para la determinación cualitativa y cuantitativa de vitamina E (a -tocoferol en muestras de Pinus caribaea Morelet y Eucaliptus sp. Los extractos etereos se cromatografiaron en placas preelaboradas con silicagel, para lo cual se empleó cloroformo como fase móvil. La cuantificación se realizó por detección densitométrica de las manchas del compuesto mediante un sistema controlado por computadora en los modos de reflexión/absorbancia a una longitud de onda de 280 nm. Este trabajo constituye el primer reporte de la presencia de esta sustancia en Cuba, encontrándose la vitamina E en concentraciones de 177 y 2 400 mg/kg de droga seca en pino y eucalipto respectivamente.A fast methods of thin layer chromatography for the qualitative and quantitative determination of vitamine E (a -tocopherol in samples of Pinus caribaea Morelet and Eucalyptus sp. is presented. The ethereal extracts were chromatographied in plates preelaborated with silica gel, for which chloroform was used as a mobile phase. Quantification was carried out by densitometric detection of the compound stains, using a computer controlled system in the modes of reflexion absorbability at a wave longitude of 280 nm. This paper is the first report about the presence of this substance in Cuba. Vitamin E has been found in concentrations of 177 and 2 400 mg/kg of dry drug in pine and eucalyptus respectively.

  10. A consensus genetic map for Pinus taeda and Pinus elliottii and extent of linkage disequilibrium in two genotype-phenotype discovery populations of Pinua taeda

    Science.gov (United States)

    Jared W. Westbrook; Vikram E. Chhatre; Le-Shin Wu; Srikar Chamala; Leandro Gomide Neves; Patricio Munoz; Pedro J. Martinez-Garcia; David B. Neale; Matias Kirst; Keithanne Mockaitis; C. Dana Nelson; Gary F. Peter; John M. Davis; Craig S. Echt

    2015-01-01

    A consensus genetic map for Pinus taeda (loblolly pine) and Pinus elliottii (slash pine) was constructed by merging three previously published P. taeda maps with a map from a pseudo-backcross between P. elliottii and P. taeda. The consensus map positioned 3856 markers via...

  11. Productividad aérea y ciclo de nutrientes en plantaciones de Pinus taeda L. en el norte de la provincia de Misiones, Argentina

    OpenAIRE

    Pérez, Carolina Alejandra; Goya, Juan F.; Bianchini, Flavia; Frangi, Jorge Luis; Fernández, Roberto

    2006-01-01

    Se estimó la productividad primaria aérea neta (PPAN), ciclado y eficiencia del uso de nutrientes (EUN) de 3 plantaciones experimentales de Pinus taeda L. de 20 años, del norte de la provincia de Misiones, Argentina. La PPAN fue 34,8±1,5Mg·ha‑1·año‑1 y la caída al mantillo, 16 ±1,1Mg·ha-1·año‑1. El incremento medio anual del fuste fue 20% mayor que en plantaciones comerciales de Misiones y cercano al extremo superior del intervalo para plantaciones de pinos tropicales y subtropicales. El requ...

  12. Photosynthesis and respiration in the needles of Pinus sibirica and Pinus pumila and their putative hybrids

    Directory of Open Access Journals (Sweden)

    A.P. Zotikova

    2013-12-01

    Full Text Available A putative interspecific hybridization in Pinaceae family was investigated. Very rarely the physiological methods were involved in hybridization processes that occurs in the hybrid zones. It is well known that in most gymnosperms, the plastid genome is inherited from the paternal component while the mitochondrion is inherited from the maternal one. Therefore functioning pattern of organelles in the hybrid plant can suggest parent, from which they were inherited. The aim of this study was to indirectly establish the inheritance energy-transducing organelles (mitochondria, chloroplast according to their functioning. Current year needles from Siberian Stone Pine (Pinus sibirica Du Tour and Japanese Stone Pine (Pinus pumila (Pall. Regel as parent species and their putative hybrids were collected from Baikal Region. The photosynthesis rate was determined by using the spectrophotometer. The study of emission CO2 under dark respiration of needle was conducted with laser optical-acoustic gasanalyzer. The quantity was measured at 1, 2 and 3 hour after experiment start. The rate of the photoreduction ferricyanide potassium was characterized by the primary photochemical processes activity at the level of photosystem II. Comparison of pure species was shown that Japanese Stone Pine had higher functional activity of chloroplast as compared with SiberianStone Pine in spite of the fact that they are growing in similar environment conditions. Two of three analyzed hybrids had decreasedactivity of their chloroplasts. Unfortunately, in this case we can't conclude if the chloroplasts were inherited from Siberian Stone Pine orfrom Japanese Stone Pine. Chloroplast activity of the third hybrid was approximately similar to that of Japanese Stone Pine suggesting thatits chloroplasts were inherited from this parent. Consequently, the Siberian Stone Pine and the Japanese Stone Pine were maternal and paternal, respectively parents of

  13. INFLUENCIA DEL POLIETILÉN GLICOL SOBRE LOS PARÁMETROS DE PRODUCCIÓN DE GAS in vitro EN CUATRO FORRAJERAS NATIVAS CONSUMIDAS POR EL VENADO COLA BLANCA

    Directory of Open Access Journals (Sweden)

    Tilo Gustavo Domínguez-Gómez

    2011-01-01

    Full Text Available La composición química y la fermentación in vitro se determinó en hojas tratadas con y sin polietilén glicol (PEG, de arbustivas nativas del noreste de México como: Acacia amentacea, Celtis pallida, Forestiera angustifolia y Parkinsonia texana colectadas en agostaderos manejados sin disturbio. Las colectas se realizaron en enero y abril de 2009 en tres municipios (China, Linares y Los Ramones del estado de Nuevo león, México. La composición química y la cinética de la fermentación in vitro variaron ampliamente entre sitios, plantas y entre muestreos dentro de especies. Sólo A. amentacea (18 % y P. texana (8 %, que tuvieron alto contenido de taninos condensados (TC, aumentaron significativamente los parámetros de producción de gas y la energía metabolizable (EM después del tratamiento con PEG. Celtis pallida y F. angustifolia tuvieron el más bajo contenido de lignina (LDA y TC; sin embargo, C. pallida resultó con la más alta fermentación in vitro. Lo anterior se podría explicar por diferencias entre arbustivas respecto a las características genéticas relacionadas con la actividad de los metabolitos secundarios de las plantas. Todas las plantas tuvieron alto valor nutricional para el venado cola blanca debido a que su aparato digestivo tiene mecanismos para neutralizar TC.

  14. Sexual stability in the nearly dioecious Pinus johannis (Pinaceae).

    Science.gov (United States)

    Flores-Rentería, Lluvia; Molina-Freaner, Francisco; Whipple, Amy V; Gehring, Catherine A; Domínguez, C A

    2013-03-01

    Even though dioecy is a dominant sexual system among gymnosperms, little is known about its evolutionary history. Pinus johannis may represent a model system because unisexual and monoecious individuals compose its populations. The presence of unisexual individuals in other Pinus species is a consequence of sexual lability. Here we determined whether P. johannis represents the first example of a dioecious or nearly dioecious reproductive system in conifers by evaluating its sexual stability. • To assess the stability of sexual expression, we quantified the proportion of male vs. female reproductive structures produced by trees over multiple years and tested for the presence of sexual dimorphism. Sexual lability hypotheses were also examined by looking at the relationship between environmental factors and sexual expression and by comparing the reproductive behavior of P. johannis with its closest labile relative, P. edulis. • Pinus johannis is nearly dioecious: ~99% of individuals are unisexual or express a low proportion of the opposite gender with few changes in sexual expression through time. We found sexual dimorphism consistent with sexual stability. Sexual expression did not vary with tree size/age, abiotic environment, or herbivore removal, providing evidence against sexual lability. Individuals of P. johannis tended to produce only male or female strobili, whereas those of P. edulis were mainly monoecious with a gradient in the female to male strobili ratio. • This study represents the first report of a nearly stable dioecious Pinus species. The variety of sexual morphs coexisting in the same population makes P. johannis a model for studying the evolution of dioecy in gymnosperms.

  15. Vermicompost enhances germination of the maritime pine (Pinus pinaster Ait.)

    OpenAIRE

    Lazcano, Cristina; Sampedro Pérez, Luis; Zas Arregui, Rafael; Domínguez, Jorge

    2010-01-01

    We investigated the effect of vermicompost on the germination and early development of six different progenies of the maritime pine (Pinus pinaster Ait.). We compared the effects of incorporating solid vermicompost into the potting media to those of vermicompost water extract to asses the extent of not physically-mediated positive effects. The incorporation of vermicompost in the growing media of maritime pine increased germination by 16%, and particularly, addition of vermicom...

  16. Estudio comparativo in vitro de diferentes métodos de profilaxis y acondicionamiento del esmalte en la capacidad de adhesión de un sellador de fosas y fisuras

    Directory of Open Access Journals (Sweden)

    María Natalia Mandri

    Full Text Available Resumen El objetivo de este estudio comparativo in vitro fue evaluar y comparar el efecto de diferentes métodos de profilaxis y acondicionamiento del esmalte, en la capacidad de adhesión de un sellador de fosas y fisuras fotopolimerizable. La porción coronaria de 50 premolares extraídos se seccionaron en dos mitades (vestibular y lingual y se incluyeron en bloques de acrílico. Las muestras se dividieron en cinco grupos de acuerdo con los métodos de profilaxis: sin profilaxis, piedra pómez, gluconato de clorhexidina, pasta fluorada y limpiador por bicarbonato (air prophy; y técnicas de acondicionamiento del esmalte: grabado ácido con ácido fosfórico al 37% y adhesivo de autograbado. La fuerza de adhesión del sellador se midió en una máquina Instron® mediante una prueba de tracción. Los resultados no demostraron diferencias significativas en la resistencia a la tracción de acuerdo a las técnicas de acondicionamiento del esmalte utilizadas. En relación con los métodos de profilaxis estudiados, se observó que existieron diferencias significativas en los grupos tratados con pasta fluorada y limpiador por bicarbonato

  17. Glacial Refugium of Pinus pumila (Pall.) Regel in Northeastern Siberia

    Energy Technology Data Exchange (ETDEWEB)

    Shilo, N A; Lozhkin, A V; Anderson, P M; Brown, T A; Pakhomov, A Y; Solomatkina, T B

    2007-02-10

    One of the most glowing representatives of the Kolyma flora [1], ''Pinus pumila'' (Pall.) Regel (Japanese stone pine), is a typical shrub in larch forests of the northern Okhotsk region, basins of the Kolyma and Indigirka rivers, and high-shrub tundra of the Chukchi Peninsula. It also forms a pine belt in mountains above the forest boundary, which gives way to the grass-underbrush mountain tundra and bald mountains. In the southern Chukchi Peninsula, ''Pinus pumila'' along with ''Duschekia fruticosa'' (Rupr.) Pouzar and ''Betula middendorffii'' Trautv. et C. A. Mey form trailing forests transitional between tundra and taiga [2]. Pinus pumila pollen, usually predominating in subfossil spore-and-pollen spectra of northeastern Siberia, is found as single grains or a subordinate component (up 2-3%, rarely 10%) in spectra of lacustrine deposits formed during the last glacial stage (isotope stage 2) in the Preboreal and Boreal times of the Holocene. Sometimes, its content increases to 15-22% in spectra of lacustrine deposits synchronous to the last glacial stage near the northern coast of the Sea of Okhotsk [3], evidently indicating the proximity of Japanese stone pine thickets.

  18. Biological invasion of Pinus ponderosa and Pinus contorta: case study of a forest plantation in Northwestern Patagonia; Invasion biologica de Pinus ponderosa y Pinus contorta: estudio de caso de una plantacion en la Patagonia noroccidental

    Energy Technology Data Exchange (ETDEWEB)

    Dezzotti, A.; Sbrancia, R.; Mortoro, A.; Monte, C.

    2009-07-01

    In the Southern Hemisphere, Pinus species from plantations can bring about processes of biological invasion that cause significant and permanent changes on the structure and functioning of surrounding natural ecosystems. The invasive character of Pinus ponderosa (P) and Pinus contorta (C) was examined for a 20-year old plantation located in the Alicura Forest Station (40 degree centigrade 40' S and 71 degree centigrade 00' W), through the analysis of abundance, age and spatial structures, and dispersal of natural regeneration. Seedlings and saplings were located largely within the plantation boundaries, and exhibited a density of 6.9 ind / ha (41 % for P and 59 % for C), a clustered spatial pattern with clumps dispersed not randomly, and a mean dispersal rate of 9.5 m / yr for P. ponderosa and 5.4 m / yr for P. contorta. Both species were invading the adjacent area, according to technical criteria based on ecological responses. However, regeneration niche is strongly hindering tree establishment and dispersal, probably due to high plant cover, presence of vertic soils, and absence of ectomycorrhizal fungi. These results can contribute to predict the capability of P. contorta and P. ponderosa to become invasive, in order to maximize the positive balance of forestry based on these species in northwestern Patagonia. (Author) 50 refs.

  19. Estudio in vitro del sellado de conductos obturados con gutapercha y sellador AH26 mediante la técnica de la condensación lateral de la gutapercha en frio.

    OpenAIRE

    González Calvo, Javier José

    2005-01-01

    RESUMEN Se realizó in vitro la endodoncia de cuarenta y dos dientes humanos uniconductales, obturando cuarenta con gutapercha y sellador AH 26 mediante la técnica de condensación lateral de la gutapercha en frío, y dos únicamente con sellador. Se estudió la desadaptación de los materiales de obturación a las paredes dentinarias en función de la presencia / ausencia de barro dentinario y la viscosidad del sellador. Las muestras se sometieron a un proceso de descalcificación y...

  20. Efecto in vitro del látex de Ficus insipida sobre la cascada de la coagulación sanguínea

    National Research Council Canada - National Science Library

    Fernando Concha-Benavente

    2010-01-01

    ... solo superficialmente su efecto anticoagulante in vitro. Objetivo: Comprobar el efecto anticoagulante in vitro y determinar la vía de la coagulación sobre la que actúa el látex de Ficus insípida. Material y métodos: Se obtuvo...

  1. SELEÇÃO PRECOCE DE PROGÊNIES DE PINUS RADIATA A SPHAEROPSIS SAPINEA

    Directory of Open Access Journals (Sweden)

    Paula Rachel Rabelo Corrêa

    2012-01-01

    Full Text Available Sphaeropsis sapinea is an important pathogen for some Pinus radiata causing tip blight and death of trees, in commercial plantings. This study was developed to select Pinus radiata progenies resistant to the pathogen, for new plantings in Brazil. Seedlings tips of 16 Pinus radiata progenies were inoculated with three pathogen monosporic isolates of Sphaeropsis sapinea, under greenhouse conditions. Lesions length and tip blight degree were evaluated. Responses of progenies to the pathogen varied according to tested isolate. The results indicated that early selection methodology was adequate to select Pinus radiata progenies resistant against Sphaeropsis sapinea. Average heritability of the progenies varied according to tested isolate and the selection criterion used.

  2. Variabilidad de la Frecuencia Cardíaca del corazón séptico in situ e in vitro.

    OpenAIRE

    Paola Contreras; Migliaro, Eduardo R.; Bruno Suhr

    2014-01-01

    Las oscilaciones normales de la duración del ciclo cardiaco se denominan Variabilidad de la Frecuencia Cardíaca y están determinadas principalmente por la influencia del sistema nervioso autónomo sobre el nodo sinusal. Una reducción de dicha variabilidad  se ha asociado con diversas patologías, incluida la sepsis.  Investigamos si la reducción de la Variabilidad de la Frecuencia Cardíaca en sepsis experimental se debe a cambios intrínsecos del corazón. Se registró el ECG de ratas antes y ...

  3. Taishan Pinus massoniana pollen polysaccharide inhibits subgroup J avian leucosis virus infection by directly blocking virus infection and improving immunity.

    Science.gov (United States)

    Yu, Cuilian; Wei, Kai; Liu, Liping; Yang, Shifa; Hu, Liping; Zhao, Peng; Meng, Xiuyan; Shao, Mingxu; Wang, Chuanwen; Zhu, Lijun; Zhang, Hao; Li, Yang; Zhu, Ruiliang

    2017-03-13

    Subgroup J avian leucosis virus (ALV-J) generally causes neoplastic diseases, immunosuppression and subsequently increases susceptibility to secondary infection in birds. The spread of ALV-J mainly depends on congenital infection and horizontal contact. Although ALV-J infection causes enormous losses yearly in the poultry industry worldwide, effective measures to control ALV-J remain lacking. In this study, we demonstrated that Taishan Pinus massoniana pollen polysaccharide (TPPPS), a natural polysaccharide extracted from Taishan Pinus massoniana pollen, can significantly inhibit ALV-J replication in vitro by blocking viral adsorption to host cells. Electron microscopy and blocking ELISA tests revealed that TPPPS possibly blocks viral adsorption to host cells by interacting with the glycoprotein 85 protein of ALV-J. Furthermore, we artificially established a congenitally ALV-J-infected chicken model to examine the anti-viral effects of TPPPS in vivo. TPPPS significantly inhibited viral shedding and viral loads in immune organs and largely eliminated the immunosuppression caused by congenital ALV-J infection. Additionally, pre-administration of TPPPS obviously reduced the size and delayed the occurrence of tumors induced by acute oncogenic ALV-J infection. This study revealed the prominent effects and feasible mechanisms of TPPPS in inhibiting ALV-J infection, thereby providing a novel prospect to control ALV-J spread.

  4. Relationships between the dynamics of Pinus halepensis Mill. and Pinus sylvestris L. plantations and environmental parameters: a basis for sustainable management of stands

    OpenAIRE

    Bueis Mellado, Teresa de los

    2017-01-01

    Pinus halepensis Mill. y Pinus sylvestris L. son especies ampliamente utilizadas en la repoblación de suelos pobres y degradados en España. Pinus halepensis es una especie mediterránea adaptada a la sequía y a un amplio rango de sustratos, capaz de colonizar suelos muy pobres mejorándolos, fomentando el crecimiento de especies nativas como Quercus ilex L. o Quercus faginea Lam. A pesar de lo anterior, no ha sido estudiado en demasiada profundidad debido al escaso interés económico...

  5. Producción de chapa torneada de Pinus ayacahuite para la elaboración de tabletas para lápices

    Directory of Open Access Journals (Sweden)

    D. Zavala-Zavala

    2005-01-01

    Full Text Available Para la producción de tabletas para lápices, la industria mexicana utiliza el método de asierre y rebanado. Este estudio es el primer intento de producción de chapa torneada de 5 mm de espesor para la obtención de tabletas con trocería de Pinus ayacahuite Ehr. Se realizaron cuatro ensayos de programas de acondicionamiento en las instalaciones de la planta piloto de la División de Ciencias Forestales de la Universidad Autónoma Chapingo. Las trozas se acondicionaron en fosas con agua calentada a temperaturas controladas y la temperatura de desenrollado se midió en el centro de las trozas, al final del proceso. Para lograr el espesor de chapa indicado, se ajustaron las aberturas vertical y horizontal de la cuchilla y contracuchilla, a 5 y 2 mm, respectivamente. Se determinó que la temperatura óptima de corte para Pinus ayacahuite Ehr es de 48 a 52 °C y se sugiere utilizar temperaturas del agua de 50 a 75 °C en un periodo de 20 h. El método de producción de tabletas por chapa torneada supera hasta en 50% el coeficiente de aprovechamiento del sistema tradicional de la industria lapicera mexicana.

  6. Determinación de las condiciones de crecimiento in vitro de una cepa probiótica (Lactobacillus delbruekii subsp. bulgaricus aislada del tracto intestinal de terneros (Bos taurus

    Directory of Open Access Journals (Sweden)

    José Ávila

    2010-07-01

    Full Text Available El objetivo de este estudio fue conocer el efecto de la temperatura, pH y la concentración de inóculo sobre el crecimiento in vitro de una cepa probiótica (Lactobacillus delbruekii subsp. bulgaricus aislada del intestino delgado de terneros. Para ello se utilizó en el primer caso, el método de superficie de respuesta para determinar las condiciones de pH y temperatura óptimas de la cepa, y en el segundo, un diseño experimental completamente aleatorizado considerando el factor concentración de inóculo inicial a seis niveles, donde el crecimiento (en medio De Man, Rogosa y Sharpe expresado en unidades de Absorbancia, constituyó la variable respuesta; presentándose una máxima respuesta (0,806 uA para 5 % de concentración inicial de inóculo. Los valores de pH y temperatura utilizados permitieron el establecimiento de una zona óptima de crecimiento in vitro en los siguientes intervalos, pH entre 5,1 y 5,6; y temperatura entre 38,0 y 41,5 °C, donde el máximo crecimiento se obtuvo a pH 5,31 y a 39,38 °C. Por otro lado se encontraron incrementos significativos del crecimiento in vitro (p < 0,05 a medida que se utiliza menor concentración de inóculo inicial. Con la información obtenida se espera incrementar los rendimientos de la cepa y así promover la producción de productos probióticos para el consumo animal en Venezuela.

  7. Parámetros genéticos e interacción genotipo x ambiente en el crecimiento de progenies reintroducidas y nativas de Pinus patula.

    OpenAIRE

    Gómez Martínez, Procoro

    2013-01-01

    Pinus patula Schiede ex Schltdl. et. Cham., es una especie nativa de México ampliamente utilizada en plantaciones comerciales en diferentes regiones del mundo; la especie ha sido mejorada en el extranjero, y es necesario evaluar el material al ser reintroducido a su país de origen. Se comparó el crecimiento de 36 familias de medios hermanos seleccionadas en Colombia y Sudáfrica, reintroducidas en México (grupo R) y 36 familias de medios hermanos escogidas fenotípicamente en rodales naturales ...

  8. Variabilidad y modelización geoestadística de producción de resina y madera de Pinus pinaster Ait. en los montes de Segovia

    OpenAIRE

    Nanos, Nikolaos

    2001-01-01

    En la presente memoria se estudia la variabilidad y se prueba el potencial de la geoestadística en la modelización espacial de dos productos forestales: madera y resina. También se discuten algunos aspectos sobre la variabilidad temporal de los mismos. La prueba se realiza en los pinares en resinación de Pinus pinaster Ait. de la provincia de Segovia, se disponen de producción de resina de dos campañas de resinación, mientras que para la producción de madera se utilizaron datos del segundo in...

  9. CONTINUIDADE ESPACIAL DE CARACTERÍSTICAS DENDROMÉTRICAS DE PINUS (Pinus pinaster Aiton E DE ATRIBUTOS DO SOLO / SPATIAL CONTINUITY OF DENDROMETRICS CHARACTERISTICS OF PINUS (Pinus pinaster Aiton AND SOIL ATTRIBUTES

    Directory of Open Access Journals (Sweden)

    GLÉCIO M. SIQUEIRA

    2009-04-01

    Full Text Available O objetivo deste trabalho foi avaliar a relação e a continuidade espacial de características dendrométricas de pinus (Pinus pinaster Aiton e de atributos do solo. As amostragens foram realizadas em uma área de 0,25 ha cultivada com Pinus pinaster em Muras (Província de Lugo, Galícia, Espanha. Os atributos amostrados foram: o diâmetro da base das árvores (DB; o diâmetro a 0,30 m acima da base (D30; o diâmetro a altura do peito (a 1,3 m de altura, DAP, altura das árvores (ALT, profundidade do perfil do solo (PROF e a condutividade elétrica aparente do solo a 0,3 m de profundidade (CEa-H e a 1,5 m de profundidade (CEa-V. O uso da geoestatística permitiu caracterizar a variabilidade espacial de todos os atributos em estudo, com exceção da profundidade da rocha que apresentou efeito pepita puro. Os dados apresentaram moderada razão de dependência espacial entre amostras. Os mapas de variabilidade espacial não demonstram uma relação entre os mapas dos atributos de solo e planta estudados.Palavras-Chave: geoestatística; silvicultura de precisão; condutividade elétrica aparente do solo.

  10. EVALUACIÓN DEL COMPORTAMIENTO IN VITRO DE RECUBRIMIENTOS DE HIDROXIAPATITA DEPOSITADOS MEDIANTE PROYECCIÓN TÉRMICA POR COMBUSTIÓN OXIACETILÉNICA SOBRE UN SUSTRATO DE Ti6Al4V

    Directory of Open Access Journals (Sweden)

    HAMILTON COPETE

    2013-01-01

    Full Text Available Recubrimientos de Hidroxiapatita sintética producida por precipitación y calcinación a 850 °C fueron depositados sobre sustratos de Ti6Al4V mediante proyección térmica por combustión. Las fases presentes en el material sintetizado y en los recubrimientos elaborados fueron determinadas mediante Difracción de Rayos X. Los recubrimientos fueron evaluados en condiciones in vitro con fluido fisiológico a 37 °C que simula el plasma humano, por periodos de 3, 7, 15 y 30 días. La superficie de los recubrimientos fue caracterizada antes y después de los ensayos in vitro mediante Microscopía Electrónica de Barrido y por Barrido de Energía Dispersiva. La concentración de iones de fosfato y de calcio en el fluido fisiológico fue determinada mediante espectrofotometría. Los resultados de las pruebas in vitro sugieren la acción de dos mecanismos: disolución del recubrimiento en el fluido fisiológico y posterior precipitación de cristales de calcio y fósforo sobre la superficie de la capa de Hidroxiapatita.

  11. Modeling Tree Mortality Following Wildfire in Pinus ponderosa Forests in the Central Sierra Nevada of California

    Science.gov (United States)

    Susan G. Conard; Jon C. Regelbrugge

    1993-01-01

    Abstract. We modeled tree mortality occurring two years following wildfire in Pinus ponderosa forests using data from 1275 trees in 25 stands burned during the 1987 Stanislaus Complex fires. We used logistic regression analysis to develop models relating the probability of wildfire-induced mortality with tree size and fire severity for Pinus ponderosa, Calocedrus...

  12. Development of site index curves for Pinus kesiya in the Philippines ...

    African Journals Online (AJOL)

    Development of site index curves for Pinus kesiya in the Philippines. RIC Lumbres, YJ Lee, YO Seo, FG Calora JR. Abstract. This study was conducted to develop a height–age growth model and site index curves for site quality evaluation of old secondary-growth stands of Pinus kesiya in the northern Philippines.

  13. Damping-off Disease of two Pulp and Paper Forest Species ( Pinus ...

    African Journals Online (AJOL)

    Anexperiment was conducted to assess the effect of four sowing media (ground granite, sharp river sand, top soil and saw dust) on the incidence of damping-off disease in two pine species, Pinus caribaea and Pinus oocarpa during the wet and dry seasons of 2006/07. The experiment was laid out in a completely ...

  14. Evolution and biogeography of Pinus radiata, with a proposed revision of its quaternary history

    Science.gov (United States)

    Constance I. Millar

    1999-01-01

    The genus Pinus evolved about 100 million years ago, spreading from centres in eastern North America and western Europe throughout middle latitudes of the supercontinent Laurasia. Many early subsections of Pinus are recorded from fossil remains ofthis period, but it is not until the early Tertiary, when the genus was fragmented by...

  15. Using flotation in ethanol to separate filled and empty seeds of Pinus ...

    African Journals Online (AJOL)

    USER

    2010-06-21

    Jun 21, 2010 ... seeds of Pinus nigra ssp. pallasiana. Mahmut D. Avsar. Department of ... Accepted 7 May, 2010. In this study, the separation of filled and empty seeds of Crimean pine [Pinus nigra Arn. ssp. pallasiana ..... stratification of thinleaf alder and water birch seeds from New Mexico. Native Plants J. 3(2): 142-150.

  16. Resistance to white pine blister rust in Pinus flexilis and P

    Science.gov (United States)

    Anna W. Schoettle; Richard A. Sniezko; Angelia Kegley; Jerry Hill; Kelly S. Burns

    2010-01-01

    The non-native fungus Cronartium ribicola, that causes white pine blister rust (WPBR), is impacting or threatening limber pine, Pinus flexilis, and Rocky Mountain bristlecone pine, Pinus aristata. In the Southern Rockies, where the rust invasion is still expanding, we have the opportunity to be proactive and prepare the landscape for invasion. Genetic...

  17. Studies on ovulate strobili and seed production of Pinus patula in ...

    African Journals Online (AJOL)

    Studies on ovulate strobili and seed production of Pinus patula in KwaZulu-Natal: scientific paper. ... Southern Forests: a Journal of Forest Science ... Cone and seed production studies in Pinus patula were conducted by the Institute for Commercial Forestry Research (ICFR) in KwaZulu-Natal as part of the improvement ...

  18. Efecto neuroprotector del extracto hidroalcohólico de Piper aduncum “matico” en un modelo in vitro de neurodegeneración

    Directory of Open Access Journals (Sweden)

    César Zaa

    2013-06-01

    Full Text Available Durante los procesos neurodegenerativos la función y viabilidad de las neuronas se reduce. En particular, el incremento patológico en la concentración de calcio intracelular, la alteración de la plasticidad sináptica y apoptosis están implicados en la Enfermedad de Alzheimer (EA, paradigma de un proceso neurodegenerativo, relacionado con la pérdida progresiva de las funciones cognitivas. Para evaluar el efecto neuroprotector de Piper aduncum“matico” se indujo el daño con el péptido Aβ a células cultivadas. Igualmente, células hipocam-pales fueron tratadas con el Aβ, y se evaluó viabilidad celular, niveles de caspasa-3 y expresión de receptores NMDA en sinapsis. También se registró el influjo de calcio intracelular en tratamientos con agonista NMDA y P. aduncum. En la evaluación neuroprotectora hay una reducción de un 20,6% de caspasa-3; un aumento del 9,6% por encima del control y una recuperación del 20,86% para las proteínas NR1 y SV2 respectivamente. Además hay una reducción de más del 50% del calcio celular. Estos resultados evidencian efecto neuropro-tector de P. aduncumpara el modelo estudiado.

  19. Genetic diversity and gene exchange in Pinus oocarpa, a Mesoamerican pine with resistance to the pitch canker fungus (Fusarium circinatum)

    Science.gov (United States)

    W.S. Dvorak; K.M. Potter

    2009-01-01

    Eleven highly polymorphic microsatellite markers were used to determine the genetic structure and levels of diversity in 51 natural populations of Pinus oocarpa across its geographic range of 3000 km in Mesoamerica. The study also included 17 populations of Pinus patula and Pinus tecunumanii chosen for their resistance or susceptibility to the pitch canker fungus based...

  20. Cost optimization of the system of use of wood in natural forests de Pinus caribaea

    Directory of Open Access Journals (Sweden)

    Fidel Cándano Acosta

    2012-03-01

    Full Text Available The work was carried out in natural forests of Pinus caribaea in areas of the forest company “La Palma” in the province of Pinar del Río in Cuba. The objective of the investigation was to minimize the cost of harvesting by combining the costs of haulage and road and load patio densities. In addition, the study also takes into consideration the interaction of costs of transportation and the thickness and quality of the layer of gravel on the road. The results of the investigation have shown a significant increase in the spacing between roads and load patios with the decrease of US$0.45/m³. It was possible to improve the quality of the layer of gravel on the road without changing the construction costs significantly, which led to a cost reduction of US$1.01/m³. The overall cost considering the new system decreases in US$1.46/m³. Another significant contribution is made by decreasing the impact to the ecosystem once the reduction on road density may reach around 44% in comparison to the current density.

  1. Influence of gamma radiation on the growth and metabolism in vitro culture of potato parenchyma; Influencia de la radiacion gamma sobre el desarrollo y metabolismo del parenquina amilifero de patata cultado in vitro

    Energy Technology Data Exchange (ETDEWEB)

    Fernandez Gonzalez, J.; Monzon Matanzo, M. P.

    1977-07-01

    The present work studies the growth in vitro of the parenchyma tissues coming from control and irradiated potato tubers with doses of 3, 6, 9 and 12 Krad. At the end of a four months cultivation period the cellular proliferation, respiratory activity, content in ascorbic acid, conductivity and ph was studied. Some differences between control and irradiated tissues were observed. (Author) 22 refs.

  2. Species diversity and plastid dna haplotype distributions ofPinus Subsection Australes (pinaceae in Guerrero and Oaxaca

    Directory of Open Access Journals (Sweden)

    Alfredo Ortiz-Martínez

    2016-01-01

    Full Text Available Pinus subsección Australes es un grupo de pinos duros de América del Norte que comprende aproximadamente 29 especies de árboles importantes económicamente y ecológicamente distribuidos a lo largo de toda América del Norte y Central y las Islas Caribeñas. Estudios previos han mostrado que las especies de esta subsección a menudo comparten haplotipos de ADN de plastidio, un patrón que es atribuido a la hibridación introgresiva y la retención de polimorfismos ancestrales. Aquí describimos la diversidad de haplotipos de plastidio y la morfología para este grupo de especies en los estados de Guerrero y Oaxaca, México. Siete especies de Pinus subsección Australes son reconocidas en el área de estudio, una de las cuales, P. patulaincluye dos variedades. Siete sitios variables y nueve haplotipos fueron encontrados amplificando un fragmento de 840 p. b. de ADN de la región codificante ycf1. Se encontraron haplotipos compartidos para P. patulavar. patula, P. patula var. longipedunculata, P.herrerae y P. tecunumanii. Cuatro de los nueve haplotipos encontrados están restringidos a Oaxaca. Aunque las genealogías de genes son valiosas para estudiar la evolución de este grupo, se requieren mayor muestreo de individuos y más sitios variables para la inferencia de relaciones entre las especies.

  3. The role of endophytic fungal individuals and communities in the decomposition of Pinus massoniana needle litter.

    Directory of Open Access Journals (Sweden)

    Zhilin Yuan

    Full Text Available The role of fungal endophytes (FEs as "pioneer" decomposers has recently been recognized; however, the extent to which FEs contribute to litter loss is less well understood. The genetic and enzymatic bases of FE-mediated decomposition have also rarely been addressed. The effects of populations and individuals (with an emphasis on two dominant Lophodermium taxa of FEs on needle-litter decomposition were assessed for Pinus massoniana, a ubiquitous pine in southern China. Data from in vivo (microcosm experiments indicated that the percentage of litter-mass loss triggered by FEs was linearly correlated with incubation time and approached 60% after seven months. In vitro decomposition tests also confirmed that endophytic Lophodermium isolates caused 14-22% mass loss within two months. Qualitative analysis of exoenzymes (cellulase and laccase, important for lignocellulose degradation revealed that almost all of the Lophodermium isolates showed moderate or strong positive reactions. Furthermore, partial sequences of β-glucosidase (glycoside hydrolase family 3, GH3, laccase, and cellobiohydrolase (GH7 genes were amplified from Lophodermium isolates as "functional markers" to evaluate their potential for lignocellulolytic activity. Three different genes were detected, suggesting a flexible and delicate decomposition system rich in FEs. Our work highlights the possibility that the saprophytism and endophytism of FEs may be prerequisites to initiating rapid decomposition and thus may be key in Fes' contribution to litter decomposition, at least in the early stage. Potential indicators of the presence of core fungal decomposers are also briefly discussed.

  4. Caracterización de la actividad alergénica y enzimática de extractos somáticos producidos a partir de cultivos in vitro del ácaro Dermatophagoide farinae

    Directory of Open Access Journals (Sweden)

    Dary Luz Mendoza Meza

    2011-01-01

    Full Text Available Introduccion: El Dermatophagoides fainae (DF es un acaro cosmopolita presente en el polvo intradomiciliario. Este acaro es fuente importante de aereo-alergenos desencadenantes de asma y rinitis alergica. Objetivo: El proposito de este trabajo fue la produccion y caracterizacion de extractos alergenicos para uso en diagnostico alergologico y terapia. Metodos: Los extractos se prepararon a partir de cultivos puros in vitro de acaros. Las proteinas del acaro fueron extraidas mediante lisis osmotica. La concentracion de proteinas se determino por microensayo de Bradford y el perfil electroforetico fue identificado por SDS-PAGE. La actividad enzimatica fue evaluada con el estuche comercial Api Zym (Biomeriux R y la actividad alergenica por inmunoensayo de Dot Blot. Resultados: Se prepararon cinco lotes de extractos. La media del peso seco de material extraido fue 37,04 mg/gramo de acaros ( DE= 11,3 y la media de la cantidad de proteina fue 212,4 ¿Êg/mg de peso seco (DE= 33,3. El perfil SDS-PAGE mostro la presencia de 8 bandas comunes (21-109 kDa. Todos los extractos presentaron actividad enzimatica hidrolasa. Tambien se demostro actividad tripsina, alfa-amilasa y alfa-quimiotripsina, correspondientes a los alergenos de los acaros del grupo 3, 4 y 6 respectivamente. Todos los extractos tuvieron proteinas con capacidad de union a los anticuerpos IgE. Conclusion: Aqui presentamos la produccion de extractos del acaro DF, con actividad alergenica. Estos resultados fundamentaran la produccion a escala de extractos alergenicos de acaros desencadenantes de enfermedades alergicas en el humano, con fines diagnosticos y de aplicacion terapeutica.

  5. Composition and structure of Pinus koraiensis mixed forest respond to spatial climatic changes.

    Science.gov (United States)

    Zhang, Jingli; Zhou, Yong; Zhou, Guangsheng; Xiao, Chunwang

    2014-01-01

    Although some studies have indicated that climate changes can affect Pinus koraiensis mixed forest, the responses of composition and structure of Pinus koraiensis mixed forests to climatic changes are unknown and the key climatic factors controlling the composition and structure of Pinus koraiensis mixed forest are uncertain. Field survey was conducted in the natural Pinus koraiensis mixed forests along a latitudinal gradient and an elevational gradient in Northeast China. In order to build the mathematical models for simulating the relationships of compositional and structural attributes of the Pinus koraiensis mixed forest with climatic and non-climatic factors, stepwise linear regression analyses were performed, incorporating 14 dependent variables and the linear and quadratic components of 9 factors. All the selected new models were computed under the +2°C and +10% precipitation and +4°C and +10% precipitation scenarios. The Max Temperature of Warmest Month, Mean Temperature of Warmest Quarter and Precipitation of Wettest Month were observed to be key climatic factors controlling the stand densities and total basal areas of Pinus koraiensis mixed forest. Increased summer temperatures and precipitations strongly enhanced the stand densities and total basal areas of broadleaf trees but had little effect on Pinus koraiensis under the +2°C and +10% precipitation scenario and +4°C and +10% precipitation scenario. These results show that the Max Temperature of Warmest Month, Mean Temperature of Warmest Quarter and Precipitation of Wettest Month are key climatic factors which shape the composition and structure of Pinus koraiensis mixed forest. Although the Pinus koraiensis would persist, the current forests dominated by Pinus koraiensis in the region would all shift and become broadleaf-dominated forests due to the dramatic increase of broadleaf trees under the future global warming and increased precipitation.

  6. Emerging Needle Blight Diseases in Atlantic Pinus Ecosystems of Spain

    Directory of Open Access Journals (Sweden)

    Esther Ortíz de Urbina

    2016-12-01

    Full Text Available Red band needle blight caused by Dothistroma septosporum and D. pini, and brown spot needle blight caused by Lecanosticta acicola provoke severe and premature defoliation in Pinus, and subsequent reduction of photosynthetic surfaces, vitality, and growth in young and adult trees. The recurrent damage results in branch and tree death. Until recently, pine needle blight diseases have had only minor impacts on native and exotic forest trees in the North of Spain, but in the past five years, these pathogen species have spread widely and caused severe defoliation and mortality in exotic and native plantations of Pinus in locations where they were not detected before. In an attempt to understand the main causes of this outbreak and to define the effectiveness of owners’ management strategies, four research actions were implemented: a survey of the management activities implemented by the owners to reduce disease impact, the evaluation of specific symptoms and damage associated with infection, and the identification of the causative pathogenic species and their reproductive capacity. Morphological characteristics of the fungus and molecular identification were consistent with those of Lecanosticta acicola and Dothistroma spp., D. septosporum, D. Pini, and both mating types were present for the three identified pathogens. The local silvicultural management performed, mainly pruning and thinning, was not resulting in the expected improvement. The results of this study can be applied to establish guidelines for monitoring and controlling the spread of needle blight pathogens.

  7. Efecto in vitro del látex de Ficus insipida sobre la cascada de la coagulación sanguínea

    OpenAIRE

    Concha-Benavente, Fernando; Médico Cirujano miembro del Grupo de Investigación en Inmunología y Academia Peruana de Medicina Molecular. Facultad de Medicina de la Universidad Nacional de San Agustín. Arequipa.

    2011-01-01

    Es necesario investigar drogas naturales nuevas que aporten principios farmacológicos activos para ser utilizadas como una alternativa terapéutica. Por este motivo nos propusimos estudiar a Ficus insipida, cuyo látex ha sido usado como antihelmíntico durante muchos años en la amazonía, pero se investigó solo superficialmente su efecto anticoagulante in vitro. Objetivo: Comprobar el efecto anticoagulante in vitro y determinar la vía ...

  8. Meta-analysis for the volume of Pinus sylvestris in Europe

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    Kyriaki Kitikidou

    2017-01-01

    Full Text Available Las revisiones sistemáticas son herramientas importantes para la investigación de literatura objetiva, composición y análisis crítico de los resultados de estudios variantes, con una contribución excelente para aclarar temas y buscar nuevas direcciones de investigación. Este estudio tiene como objetivo la revisión sistemática de estudios independientes para la estimación del volumen de pino silvestre ( Pinus sylvestris en Europa. Para ello, se realizó un metanálisis de los modelos de estimación de volumen de árboles individuales. En este trabajo se describen las etapas del metanálisis, los métodos estadísticos, los posibles errores derivados y la heterogeneidad que se producen entre los estudios. Los resultados muestran que Italia se distingue claramente por tener volúmenes de árbol individual demasiado pequeños, mientras que Grecia y Suecia presentan grandes volúmenes. Existe una gran heterogeneidad entre los estudios, debido probablemente a las condiciones distintas de competencia suscitadas en los rodales de diferentes países de Europa, pero sin clima distinto. Más estudios podrían servir en el caso de Italia; en el caso de Grecia, un estudio con una muestra de mayor tamaño podría contribuir para fomentar la investigación.

  9. Reserva de carbono en un sistema silvopastoril compuesto de Pinus patula y herbáceas nativas

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    Manuel Oliva

    2017-01-01

    Full Text Available La investigación tuvo como objetivo determinar la reserva de carbono retenida bajo un sistema silvopastoril con Pinus patula , localizado en Amazonas, Perú. Para la estimación de carbono se utilizaron 06 plantas mediante el método directo, los fustes fueron seccionados cada 2 metros y a partir de estos se obtuvieron muestras de 5 cm de espesor previo al pesaje total. Para las ramas y hojas se realizó el pesaje total y se seleccionaron muestras de 200 g. Para la recolección de muestras de hojarasca y herbáceas nativas, se utilizó el mé todo del cuadrante y las muestras de suelo se recolectaron alrededor de cada planta. Los datos fueron obtenidos mediante la formulación de ecuaciones alométricas y selección de la mayor correlación estimada, a partir de variables como diámetro a la altura del pecho (DAP y altura del fuste (AF. Como resultado, se encontró 92,13 t/ha de carbono almacenado y 337,20 t/ha de carbono equivalente. Además, la investigación permitió establecer una ecuación que ayudó a estimar la cantidad de carbono y CO 2 en pino p átula: Biomasa seca = 0,6575*DAP 1,1794 (r 2 = 0,91 .

  10. Comportamiento in vitro del colágeno de la unión amelodentinaria en premolares humanos sometidos a altas temperaturas

    National Research Council Canada - National Science Library

    Sebastián Medina; Carlos Mejía; Freddy Moreno

    2015-01-01

    ...), para proporcionar evidencia científica que permita emplear la separación del esmalte y la dentina como un marcador fehaciente repetitivo de utilidad en los procesos de identificación odontológica y documentación de la necropsia...

  11. Importance of Abscisic Acid (ABA in the In Vitro Conservation of Cassava (Manihot esculenta Crantz Importancia del Ácido Abscísico (ABA en la Conservación In Vitro de la Yuca (Manihot esculenta Crantz

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    L. Pedro Barrueto Cid

    2008-09-01

    Full Text Available The conventional technology for in vitro plant conservation for cassava (Manihot esculenta Crantz germplasm collections is laborious due to the need for several sub-culturing procedures per year. This practice implies high costs for medium preparation, tissue culture tubes, time-consuming labor, risks of contamination, mislabeling of accession, and the need for large growth chambers. We have developed a new procedure using in vitro cultivated nodal axillary buds treated with different abscisic acid (ABA concentrations to reduce the time for recycling transplants cultivated in a SP basic nutritive medium. Nodal explants were stored for three months with ABA. Plants were obtained after nodal axillary buds were placed in SP medium without ABA. Results indicated that 20 and 30 mM ABA induced bud dormancy and delayed sprouting without affecting subsequent growth of plants after treatment.La tecnología usual para conservación in vitro de colecciones de germoplasma de yuca (Manihotesculenta Crantz es corrientemente laboriosa y emplea varias transferencias por año. Este procedimiento envuelve altos costos en preparación de medios, consumo de tiempo, riesgos de manipulación y necesidad de mucho espacio para la mantención de colecciones en cámaras de cultivos. Se desarrolló un nuevo procedimiento usando yemas axilares nodales cultivadas in vitro con diferentes concentraciones de ácido abscísico (ABA, con el objetivo de reducir los ciclos de transferencia de los cultivos mantenidos en un medio nutritivo básico tal como el SP. Los segmentos nodales fueron almacenados por tres meses en presencia de ABA. Las plantas fueron obtenidas después que los segmentos nodales fueron transferidos al medio SP sin ABA. Los resultados indican que 20 y 30 mM de ABA indujeron una completa dormancia de yemas, sin afectar el desarrollo posterior de las yemas nodales y su consecuente conversión en planta.

  12. Comparison of stem volume of Eastern white pine (Pinus strobus L. and Scotch pine (Pinus sylvestris L.

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    Petr Vaněk

    2013-01-01

    Full Text Available This study deals with mensurational and volumetric characteristics of introduced Eastern white pine (Pinus strobus in comparison with native Scotch pine (Pinus sylvestris of the similar age and habitat. Municipal forests of Hradec Králové city (an area with the natural occurrence of Scotch pine and introduced Eastern white pine; east part of Bohemia, altitude from 250 to 280 m a.s.l. belong to typological unit – nutrient-very poor, and acidic sites. There comparative analysis of basic stem variables (stem diameter at the breast-height – DBH, total tree height – H and stem volume – V of dominant trees (10 individuals with the highest DBH of both species in four suitable forest stands of age from 42 to 102 years was done. Statistical analysis showed similar trends in all forests stands. Eastern white pine compared to Scotch pine reached larger mean DBH (up to 16%. H – values do not have a clear trend across the forest stands and did not show large differences in all cases. Eastern white pine compared to Scotch pine showed also larger mean stem volume (up to 47%. Therefore, introduced Eastern white pine reached higher stem size parameters and wood production compared to native Scotch pine in studied forest district.

  13. Cross-species amplification of nuclear EST-microsatellites developed for other Pinus species in Pinus nigra

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    Šarac Zorica

    2015-01-01

    Full Text Available Due to the current lack of nuclear microsatellites (simple sequence repeats - SSRs specifically developed for Pinus nigra, an important European coniferous species, we cross-species amplified 12 EST-SSRs (expressed sequence tagged SSRs developed for other Pinus species in P. nigra in order to delineate loci which can be used for assessing levels of genetic diversity and genetic structuring in this species. We amplified these loci in individuals from seven populations from the central Balkans representing four recognized infraspecific taxa of P. nigra (ssp. nigra, var. gocensis, ssp. pallasiana, and var. banatica. Contrary to expectations on high transferability of EST-SSRs into related species, only three out of 12 tested loci were successfully amplified in P. nigra, but they displayed lack/low levels of polymorphism or generated multilocus amplification products. Thus, our estimates on levels of genetic diversity (HE = 0.183 and genetic differentiation (FST = 0.007 were based on variability of a single locus harboring four alleles only and they should be taken with cautions. Our study highlights the need for the development of high-resolution molecular markers, such as co-dominant genic or genomic SSRs or predominantly biallelic SNPs, or utilization of anonymous dominant markers, such as AFLPs, for genotyping in P. nigra. [Projekat Ministarstva nauke Republike Srbije, br. 173029 i br. 173030

  14. Ericaceous dwarf shrubs affect ectomycorrhizal fungal community of the invasive Pinus strobus and native Pinus sylvestris in a pot experiment.

    Science.gov (United States)

    Kohout, Petr; Sýkorová, Zuzana; Bahram, Mohammad; Hadincová, Věroslava; Albrechtová, Jana; Tedersoo, Leho; Vohník, Martin

    2011-07-01

    This study aimed to elucidate the relationship between ericaceous understorey shrubs and the diversity and abundance of ectomycorrhizal fungi (EcMF) associated with the invasive Pinus strobus and native Pinus sylvestris. Seedlings of both pines were grown in mesocosms and subjected to three treatments simulating different forest microhabitats: (a) grown in isolation and grown with (b) Vaccinium myrtillus or (c) Vaccinium vitis-idaea. Ericaceous plants did not act as a species pool of pine mycobionts and inhibited the ability of the potentially shared species Meliniomyces bicolor to form ectomycorrhizae. Similarly, Ericaceae significantly reduced the formation of Thelephora terrestris ectomycorrhizae in P. sylvestris. EcMF species composition in the mesocosms was strongly affected by both the host species and the presence of an ericaceous neighbour. When grown in isolation, P. strobus root tips were predominantly colonised by Wilcoxina mikolae, whereas those of P. sylvestris were more commonly colonised by Suillus and Rhizopogon spp. Interestingly, these differences were less evident (Suillus + Rhizopogon spp.) or absent (W. mikolae) when the pines were grown with Ericaceae. P. strobus exclusively associated with Rhizopogon salebrosus s.l., suggesting the presence of host specificity at the intrageneric level. Ericaceous plants had a positive effect on colonisation of P. strobus root tips by R. salebrosus s.l. This study demonstrates that the interaction of selective factors such as host species and presence of ericaceous plants may affect the realised niche of the ectomycorrhizal fungi.

  15. Determinación de las condiciones de crecimiento in vitro de una cepa probiótica (Lactobacillus delbruekii subsp. bulgaricus aislada del tracto intestinal de terneros (Bos taurus

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    José Ávila

    2010-06-01

    Full Text Available El objetivo de este estudio fue conocer el efecto de la temperatura, pH y la concentración de inóculo sobre el crecimiento in vitro de una cepa probiótica (Lactobacillus delbruekii subsp. bulgaricus aislada del intestino delgado de terneros. Para ello se utilizó en el primer caso, el método de superficie de respuesta para determinar las condiciones de pH y temperatura óptimas de la cepa, y en el segundo, un diseño experimental completamente aleatorizado considerando el factor concentración de inóculo inicial a seis niveles, donde el crecimiento (en medio De Man, Rogosa y Sharpe expresado en unidades de Absorbancia, constituyó la variable respuesta; presentándose una máxima respuesta (0,806 uA para 5 % de concentración inicial de inóculo. Los valores de pH y temperatura utilizados permitieron el establecimiento de una zona óptima de crecimiento in vitroen los siguientes intervalos, pH entre 5,1 y 5,6; y temperatura entre 38,0 y 41,5 °C, donde el máximo crecimiento se obtuvo a pH 5,31 y a 39,38 °C. Por otro lado se encontraron incrementos significativos del crecimiento in vitro (p < 0,05 a medida que se utiliza menor concentración de inóculo inicial. Con la información obtenida se espera incrementar los rendimientos de la cepa y así promover la producción de productos probióticos para el consumo animal en Venezuela.

  16. Characterization of Phenolic Compounds in Pinus laricio Needles and Their Responses to Prescribed Burnings

    Directory of Open Access Journals (Sweden)

    Lila Ferrat

    2007-07-01

    Full Text Available Fire is a dominant ecological factor in Mediterranean-type ecosystems. Management strategies include prescribed (controlled burning, which has been used in the management of several species, such as Pinus nigra ssp laricio var. Corsicana, a pine endemic to Corsica of great ecological and economic importance. The effects of prescribed burning on Pinus laricio have been little studied. The first aim of this study was to characterize total and simple phenolic compounds in Pinus laricio. The second aim was to understand: i the short term (one to three months and medium term (three years effects of prescribed burning, and ii the effects of periodic prescribed burning on the production of phenolic compounds in Pinus laricio. The first result of this study is the presence of total and simple phenolic compounds in the needles of Pinus laricio. 3-Vanillyl propanol is the major compound. After a prescribed burning, the synthesis of total phenolic compounds increases in Pinus laricio for a period of three months. Total phenolic compounds could be used as bioindicators for the short-term response of Pinus laricio needles to prescribed burning. Simple phenolic compounds do not seem to be good indicators of the impact of prescribed burning because prescribed burnings are low in intensity.

  17. ÍNDICE DE SITIO PARA PLANTACIONES DE Pinus greggii Engelm. EN METZTITLÁN, HIDALGO, MÉXICO

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    Jonathan Hernández-Ramos

    2014-01-01

    Full Text Available L os índices de productividad son esenciales en la planeación del manejo forestal sustentable. El índice de sitio es la metodología más utilizada en la clasificación de la calidad de las áreas forestales. Sin embargo, la mayoría de los macizos forestales y plantaciones no cuentan con tal información que apoye la planeación de las actividades silvícolas. Por ello, el objetivo fue estimar el índice de sitio de las plantaciones forestales de Pinus greggii Engelm., establecidas en la parte serrana del municipio de Metztitlán, Hidalgo. Los modelos de Schumacher, Chapman-Richards y Wei - bull se ajustaron con 233 observaciones de edad-altura provenientes de un análisis troncal. De acuerdo con el valor más bajo del cuadrado medio del error, el valor más alto de R 2 y la distribución de residua - les, se seleccionó el modelo de Schumacher para la construcción de la curva guía y el sistema de curvas anamórficas y polimórficas. Se establecieron cinco etiquetas de índice de sitio para las alturas 12, 14, 16, 18 y 20 m con calidades de estación V, IV, III, II y I, respectivamente. Se obtuvo buena estimación del crecimiento de la altura dominante en función de la edad, ya que considera todas las condiciones existentes dentro de las plantaciones.

  18. Potential of Pinus radiata plantations for use of harvest residues in characteristic soils of south-central Chile

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    Eduardo Cartes-Rodríguez

    2016-01-01

    Full Text Available Dada la demanda creciente sobre los residuos de cosecha para la generación de energía en plantaciones forestales de Pinus radiata en la zona centro-sur de Chile, resulta necesario disponer de modelos que permitan la estimación sitio-espec í fico de la producción de residuos. En este estudio se realizó el ajuste de modelos alométricos de estimación de la biomasa potencial de residuos de cosecha para plantaciones de P. radiata , en suelos de arenas volcánicas, cenizas volcánicas recientes y sedimentarios, considerando gradientes de manejo y productividad, en sitios característicos del centro-sur de Chile. Los modelos ajustados permitieron la estimación de factores de conversión, y modelos de estimación a nivel de árbol individual y rodal. Los resultados sugieren que los factores de conversión de biomasa fustal a biomasa potencial de residuos tienen una relación exponencial negativa con el tamaño de los individuos. Los modelos ajustados para la estimación a nivel de árbol individual, a partir de las variables diámetro y altura, sugieren que los modelos dependen del origen del suelo, mientras que para el caso de las estimaciones a nivel de rodal, el modelo ajustado resulta independiente del origen del suelo, gradiente de manejo y su productividad.

  19. Regeneración de tejido periodontal in vitro con células madre pluripotenciales de la pulpa dental (DPPSC) del tercer molar

    OpenAIRE

    Hategan, Iulia Emilia

    2016-01-01

    El periodonto está formado por varios tejidos: la encía, el ligamento periodontal, el cemento radicular y el hueso alveolar. El periodonto tiene dos funciones fundamentales: protección e inserción. La función de protección la realizan la encía y el epitelio de unión, mientras que la función de inserción se desempeña a través del ligamento periodontal, el cemento radicular y el hueso alveolar.- El ligamento periodontal es un tejido celular altamente vascularizado que rodea la raíz del dien...

  20. Estudio in vitro de la viabilidad de células Caco-2 en presencia de componentes del aceite esencial de Allium spp

    OpenAIRE

    Llana Ruiz-Cabello, M.; M. Puerto; D Gutiérrez-Praena; Pichardo, S.; A Jos; A. M. Cameán

    2013-01-01

    El aceite esencial de los componentes del género Allium, principalmente ajo y cebolla, presenta propiedades antioxidantes y antibacterianas debidas a la presencia de compuestos azufrados en su composición. La industria alimentaria ha comenzado a desarrollar nuevos sistemas de envasado activo a partir de polímeros seleccionados, a los que se incorporan aceites esenciales que, por sus propiedades, contribuyen a aumentar la vida útil de los alimentos perecederos. En este sentido, se hace necesar...

  1. Compounds that correct F508del-CFTR trafficking can also correct other protein trafficking diseases: an in vitro study using cell lines

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    Sampson Heidi M

    2013-01-01

    Full Text Available Abstract Background Many genetic diseases are due to defects in protein trafficking where the mutant protein is recognized by the quality control systems, retained in the endoplasmic reticulum (ER, and degraded by the proteasome. In many cases, the mutant protein retains function if it can be trafficked to its proper cellular location. We have identified structurally diverse correctors that restore the trafficking and function of the most common mutation causing cystic fibrosis, F508del-CFTR. Most of these correctors do not act directly as ligands of CFTR, but indirectly on other pathways to promote folding and correction. We hypothesize that these proteostasis regulators may also correct other protein trafficking diseases. Methods To test our hypothesis, we used stable cell lines or transient transfection to express 2 well-studied trafficking disease mutations in each of 3 different proteins: the arginine-vasopressin receptor 2 (AVPR2, also known as V2R, the human ether-a-go-go-related gene (KCNH2, also known as hERG, and finally the sulfonylurea receptor 1 (ABCC8, also known as SUR1. We treated cells expressing these mutant proteins with 9 structurally diverse F508del-CFTR correctors that function through different cellular mechanisms and assessed whether correction occurred via immunoblotting and functional assays. Results were deemed significantly different from controls by a one-way ANOVA (p  Results Here we show that F508del-CFTR correctors RDR1, KM60 and KM57 also correct some mutant alleles of other protein trafficking diseases. We also show that one corrector, the cardiac glycoside ouabain, was found to alter the glycosylation of all mutant alleles tested. Conclusions Correctors of F508del-CFTR trafficking might have broader applications to other protein trafficking diseases.

  2. Avaliação da qualidade de painéis compensados de Pinus maximinoi, Pinus oocarpa e Pinus tecunumannii com diferentes composições estruturais

    OpenAIRE

    Setsuo Iwakiri; Alberto Antônio Manhiça; Maria Guadalupe Lomeli Ramirez; Jorge Luis Monteiro de Matos; Ghislaine Miranda Bonduelle; José Guilherme Prata

    2012-01-01

    http://dx.doi.org/10.5902/198050985738Este trabalho teve por objetivo avaliar a influência de diferentes composições estruturais sobre as propriedades mecânicas de painéis compensados de Pinus maximinoi, Pinus oocarpa e Pinus tecunumannii. Foram produzidos em laboratório painéis compensados com sete lâminas de 3,0 mm de espessura, com composição padrão, reforço longitudinal e reforço transversal. Para a colagem de lâminas foi utilizada resina fenol-formaldeído com a seguinte formulação em par...

  3. ESTABLISHMENT OF Pinus elliottii Engelm STANDS WITH DIRECT FIELD SOWING

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    César Augusto Guimarães Finger

    2010-08-01

    Full Text Available This experiment was undertaken to test the possibility of use of direct field sowing as a method of  forest formation. The soil preparation consisted of mechanic mowing followed by cleaning of small circles of 40 cm of diameter, where four seeds of Pinus elliottii Engelm were sowed, previously stratified, and protected by a bottomless plastic glass fastened to the sowing point. The treatments tested were direct sowing, sowing of seeds involved by paper envelop and seedling plantation as testify treatment. The results were not satisfactory, however, being observed at the most 38.46% of the sowing places with seedlings after 84 days of sowing. The main factor that contribuited to the failure of the method was seedling mortality caused by water deficit and high temperatures.

  4. Interpopulation variability of Austrian pine (Pinus nigra Arnold in Serbia

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    Lučić Aleksandar

    2013-01-01

    Full Text Available Multidisciplinary studies (genetic and phytocoenological of 4 populations of Austrian pine (Pinus nigra Arnold were carried out in western and central Serbia. The obtained results gave the same inter-population arrangement in both methods. Dendograms (NTSYS differentiate at the greatest genetic distance the population of Austrian pine in Šargan compared to other populations: Crni Vrh, Goč and Studenica. Using phytocoenological analysis it was determined that population of Austrian pine in Šargan forms a community Erico-Pinetum gocensis, while the other populations form a community Seslerio rigidae-Pinetum gocensis. Multidisciplinary approach that was demonstrated within this paper presents the first studies of Austrian pine that directly link genotype dependence and environmental conditions manifested through the phytocoenological affiliation. [Projekat Ministarstva nauke Republike Srbije, br. TR 31070

  5. Needles of Pinus halepensis as biomonitors of bioaerosol emissions.

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    Amandine Galès

    Full Text Available We propose using the surface of pine trees needles to biomonitor the bioaerosol emissions at a composting plant. Measurements were based on 16S rRNA gene copy numbers of Saccharopolyspora rectivirgula, a bioindicator of composting plant emissions. A sampling plan was established based on 29 samples around the emission source. The abundance of 16S rRNA gene copies of S. rectivirgula per gram of Pinus halepensis needles varied from 104 to 102 as a function of the distance. The signal reached the background level at distances around the composting plant ranging from 2 km to more than 5.4 km, depending on the local topography and average wind directions. From these values, the impacted area around the source of bioaerosols was mapped.

  6. Needles of Pinus halepensis as biomonitors of bioaerosol emissions.

    Science.gov (United States)

    Galès, Amandine; Latrille, Eric; Wéry, Nathalie; Steyer, Jean-Philippe; Godon, Jean-Jacques

    2014-01-01

    We propose using the surface of pine trees needles to biomonitor the bioaerosol emissions at a composting plant. Measurements were based on 16S rRNA gene copy numbers of Saccharopolyspora rectivirgula, a bioindicator of composting plant emissions. A sampling plan was established based on 29 samples around the emission source. The abundance of 16S rRNA gene copies of S. rectivirgula per gram of Pinus halepensis needles varied from 104 to 102 as a function of the distance. The signal reached the background level at distances around the composting plant ranging from 2 km to more than 5.4 km, depending on the local topography and average wind directions. From these values, the impacted area around the source of bioaerosols was mapped.

  7. NUTRIENTS CONCENTRATION AND RETRANSLOCATION IN THE Pinus taeda L. NEEDLES

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    Márcio Viera

    2010-03-01

    Full Text Available Aiming at evaluating nutrients concentration and retranslocation in the Pinus taeda L. needles, this study was developed in two stands, in native grass area and in second rotation area, with same species and same age (7.5 years old in Cambará do Sul, RS. The needles were collected in plants in four orthogonal points (South, North, East and West, sampled new needles, mature needles and old needles. The material was dried in a stove, milled and chemically analyzed (macro and micronutrients. The concentrations of N, P, K, B, Cu and Zn had decreased, of Ca, Fe and Mn increased, and the Mg and S have remained constant with the age of the needles. The retranslocation rate (old-new needles was more than 50% for most nutrients, except for Mn and Fe, showed that cumulative effect and the Ca reference element.

  8. Green Synthesis of Silver Nanoparticles Using Pinus eldarica Bark Extract

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    Siavash Iravani

    2013-01-01

    Full Text Available Recently, development of reliable experimental protocols for synthesis of metal nanoparticles with desired morphologies and sizes has become a major focus of researchers. Green synthesis of metal nanoparticles using organisms has emerged as a nontoxic and ecofriendly method for synthesis of metal nanoparticles. The objectives of this study were production of silver nanoparticles using Pinus eldarica bark extract and optimization of the biosynthesis process. The effects of quantity of extract, substrate concentration, temperature, and pH on the formation of silver nanoparticles are studied. TEM images showed that biosynthesized silver nanoparticles (approximately in the range of 10–40 nm were predominantly spherical in shape. The preparation of nano-structured silver particles using P. eldarica bark extract provides an environmentally friendly option, as compared to currently available chemical and/or physical methods.

  9. Cutin fluorescence in early embryos of Pinus and Tsuga

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    Ewa Szczuka

    2014-01-01

    Full Text Available Embryos of Pinus nigra Arnold and Tsuga canadensis Carr. (Pinaceae at different stages of development were dissected from fresh, unfixed seeds and examined in a fluorescence microscope with 400 nm excitation light. The embryos of the investigated species showed cutin fluorescence after auramine 0 staining. At first the fluorescing cutin layer was formed on the apical part of the embryo with a well developed secondary suspensor, then it extended over the lateral surface of the embryo; the suspensor remained nonfluorescent. The fluorescing cutin layer occurred on the apical and side surface of the embryo, undergoing differentiation into the shoot axis and root initials. It is assumed that polarization and nutrition of the embryo may be influenced by presence of the cuticle.

  10. Quality of Pinus elliottii sawn timber from tapped forests

    Directory of Open Access Journals (Sweden)

    Pedro Henrique Gonzalez de Cademartori

    2012-12-01

    Full Text Available This study aimed to characterize the quality of Pinus elliottii sawn timber extracted from tapped forests and processed in a sawmill in São José do Norte (RS. Four butt logs and four upper logs for each of the three existing diameter grades were selected and sawed. The wood pieces were analyzed after sawing and after kiln drying. The presence of knots, which occurred due to the absence of forest management and influenced the qualitative classification of the wood pieces, was observed mainly in the samples from upper logs. The process of resin tapping contributed to a higher incidence of resin pockets in the samples from butt logs, also influencing the qualitative classification of the samples. The appearance of drying defects did not modify the classification of the wood samples from butt and upper logs.

  11. Endobacteria in some ectomycorrhiza of Scots pine (Pinus sylvestris).

    Science.gov (United States)

    Izumi, Hironari; Anderson, Ian C; Alexander, Ian J; Killham, Ken; Moore, Edward R B

    2006-04-01

    The diversity of cultivable endobacteria associated with four different ectomycorrhizal morphotypes (Suillus flavidus, Suillus variegatus, Russula paludosa and Russula sp.) of Scots pine (Pinus sylvestris) was analysed by restriction fragment length polymorphism profiling of PCR-amplified rDNA intergenic spacer regions and by sequence analyses of 16S rRNA genes. Ectomycorrhizal root tip surface-sterilization methods were developed and assessed for their efficiencies. Bacterial communities from surface-sterilized ectomycorrhizal root tips were different from those of ectomycorrhizal root tips without surface-sterilization for all the morphotypes studied. Endobacteria belonging to the genera Pseudomonas, Burkholderia and Bacillus were isolated from more than one ectomycorrhizal morphotype, whereas species of Rahnella, Janthinobacterium and Rhodococcus were only isolated from the single morphotypes of S. variegatus, R. paludosa and Russula sp., respectively. Some of the isolated endobacteria utilized fungal sugars more readily than typical plant sugars in carbon utilization assays.

  12. Evolution of tree stem taper in Pinus taeda stands

    Directory of Open Access Journals (Sweden)

    Sintia Valerio Kohler

    2016-07-01

    Full Text Available ABSTRACT: This study aimed to evaluate the evolution of stem taper of trees in Pinus taedastands using stem analysis data from 126 and 120 trees sampled from established stands in Paraná and Santa Catarina States, respectively. The integer and fractional power polynomial was fitted to estimate diameters inside bark along the stem and was used to construct taper curves by age class. The model identity test was applied to verify the change in stem form of trees. The evolution of natural and artificial form factors in the trees was also analyzed. The curves constructed from the fitted taper functions and form factors showed that the stems become more cylindrical with increasing age; the identity test indicated that, in general, the taper curves statistically differ between themselves. This showed that stratifying the data into age classes for fitting in taper functions can produce more accurate assortment estimates.

  13. Intersepsi Curah Hujan Pada Tegakan Pohon Pinus (Casuarina cunninghamia

    Directory of Open Access Journals (Sweden)

    Susi Chairani

    2013-04-01

    Full Text Available ABSTRAK. Proses tertahannya curah hujan di atas tajuk vegetasi dikenal dengan istilah intersepsi. Penelitian ini bertujuan untuk melakukan pengukuran intersepsi, air lolos (throughfall, dan aliran batang (stemflow pada tegakan pohon pinus (Casuarina cunninghamia pada kelas umur 7, 15 dan 30 tahun. Penelitian ini dilaksanakan di kawasan Taman Hutan Raya, Seulawah, Kabupaten Aceh Besar. Bahan dan alat yang digunakan pada penelitian ini adalah alat penakar curah, gelas ukur, pita ukur, klinometer, dan perangkat lunak Microsoft Excel. Berdasarkan hasil penelitian diperoleh bahwa umur pohon berpengaruh secara signifikan terhadap besarnya jumlah air lolos (throughfall dan aliran batang (stemflow. Diameter pohon berpengaruh secara signifikan untuk besarnya jumlah air lolos (throughfall dan aliran batang (stemflow. Umur pohon sangat mempengaruhi tingkat kepadatan tajuk dimana semakin lebat tajuk pohon maka intersepsi semakin besar. Bentuk daun pinus yang berbentuk jarum yang tumbuh dengan rapat juga mempengaruhi intersepsi. Rainfall Interception On The Stands of Pine Tree (Casuarina cunninghamia ABSTRACT. The process of rainfall retention above the canopy of vegetation is known as interception. This study aimed to measure interception, throughfall, and stemflow on pine trees (Casuarina cunninghamia at different ages 7, 15 and 30 years old, respectively. This research was conducted in the area of Taman Hutan Raya, Seulawah, Aceh Besar. Materials and tools used in this study were an automatic raingauge, measuring cups, measuring tape, clinometer, and Microsoft Excel software. Based on the research, the results showed that the age of the tree had significantly influenced the throughfall and stemflow. The tree’s diameter had significant influence on the large amount of throughfall and stemflow. The tree’s age greatly affected the density of tree canopy. The more dense the tree canopy, then the greater interception the tree had. The pine needle

  14. Citotossicity in immune system's cells exposed in vitro to a microwave electromagnetic field in two different exposure systems operating at mobile telephones frequencies; Citotossicita' in cellule del sistema immunitario esposte in vitro al campo elettromagnetico a microonde in due diversi sistemi espositivi operanti alle frequenze della telefonia mobile

    Energy Technology Data Exchange (ETDEWEB)

    Scarcella, E.; Ardoino, L.; Galloni, P.; Mancini, S.; Marino, C. [ENEA, Divisione Protezione dell' Uomo e degli Ecosistemi, Centro Ricerche Casaccia, Rome (Italy)

    2001-07-01

    The large growth in the use of cellular telephones has led to an increasing effort, by national and international Research Institutes, in the studies dealing with EM fields' toxicological effects and interaction mechanisms in relation to various biological systems. In order to that, in the labs of ENEA Casaccia - Human and ecosystems protection division, Toxicology and biomedical sciences section - within the research program on biological effects of electromagnetic fields, a new experimental project has been performed; its main subject has been the evaluation of possible in vitro functionality alterations in a stabilized murine macrophages cell line (RAW 264.7), after exposure to 900 MHz EM radiation. Furthermore, the standardization of two different exposure systems, working at 900 MHz in a large power density range (1 to 100 W/m{sup 2}), has bee carried out, in order to ensure homogeneity of electromagnetic field exposure, correct incubation conditions and reproducibility of experiments. Both the standardization procedures and the experimental results, which showed significant differences on cell growth and functionality only with the higher level exposure (100 W/m{sup 2}), with recovery 48 hours after exposure, will be useful to experimental procedures in progress about deepening on in vitro toxicological evaluations. [Italian] Il forte impulso dell'utilizzo di telefoni cellulari ha richiesto, da parte dei principali organi di ricerca nazionali ed internazionali, l'approfondimento degli studi per la valutazione di implicazioni tossicologiche e per lo studio dei meccanismi d'interazione del campo EM con diversi sistemi biologici. A tal proposito e' stata intrapresa nei laboratori ENEA Casaccia, Divisione Protezione dell'Uomo e degli Ecosistemi - Sezione Tossicologia e Scienze Biomediche (PRO-TOSS), nell'ambito dell'attivita' di ricerca sugli effetti biologici dei campi elettromagnetici, una nuova linea di

  15. Evaluación de la repelencia al agua de algunos Andisols de Antioquia bajo cobertura de Pinus patula

    Directory of Open Access Journals (Sweden)

    Herrón O. Francisco E.

    1991-12-01

    Full Text Available En 115 hectáreas de Andisols bajo cobertura de Pinus panda de la quebrada Piedras Blancas, se encontró repelencia severa al agua en capas con espesores entre 4 y 12 centímetros, sueltas, secas y minerales, distribuídas en parches. Los valores de hidrofobicidad obtenidos variaron entre 24 y mas de 10800 segundos, por el método WDPT; entre 0.2 y 5.2 molar, por el método MED y entre 65.5 y 134 grados, por el método del ángulo de contacto. No se encontró correlación significativa estadísticamente entre el espesor de la capa de acículas acumulada sobre el suelo y el grado de repelencia desarrollado por éste, aunque s�� la hubo entre el espesor de la capa de acículas y el espesor alcanzado por los materiales hidrofóbicos. La descomposición de raíces y acículas, los exhudados radiculares y los hongos presentes en el suelo pueden ser las principales fuentes de las sustancias hidrofóbicas que se están acumulando en aquel.Hydrophobicity of soils is present below an Andisols artificial forest of Pinus panda in the Piedras Blancas basin. In the surveyed 115 hectares the hydrophobic bed forms a discontinuous layer wich presents between 4 and 12 centimeters thick. It is recognized by their dusty and dry character. All the soils analyzed present strong repellence to water. Hydrophobicity values ranged from 24 to higher than 10800 seconds for the WDPT method; 0.2 to 5.2 molar for the MED method, with the contact angle method were obtained values between 65.5 and 134 degrees. No significant statistical correlation was found between the thick of the litter bed accumulated over the soil and the experimental hydrophobicity value, but with the thick of the hydrophobic bed had a strong positive correlation. It is believed that the decaying of root residues and litter bed, beside the root exhudates of Pinus panda are the main producers of hydrophobic substances.

  16. Evaluación del potencial probiótico de bacterias ácido lácticas para reducir el colesterol in vitro

    Directory of Open Access Journals (Sweden)

    Clementina Cueto

    2012-01-01

    Full Text Available El co nsumo diario de alimentos probióticos reduce los niveles de colesterol sérico en un 3%, valor significativo para la prevención de la hipercolesterolemia, factor de riesgo de enfermedad cardiovascular y causal de mortalidad. Algunas especies del género Lact obacillus , utilizadas en la industria alimentaria como probiótico, reducen el colesterol sérico por dos mecanismos, la adsorción de colesterol y producción de la enzima hidrolasa de sales biliares. El objetivo del estudio fue evaluar el potencial probiótic o de un grupo de bacterias lácticas aisladas de suero costeño. Se aislaron 53 cepas a partir de nueve muestras de suero costeño; el potencial probiótico de las bacterias se evaluó por tolerancia a pH 2.0, 0.3% de sales biliares y sensibilidad a vancomicina y cefoxitin. Se seleccionaron cinco microorganismos y se identificaron molecularmente, como Lactobacillus fermentum . La capacidad de adsorber el colesterol medida por el método de Kimoto, presentó disminución de 53.06 ± 2.69 μg.mL - 1 para la cepa K73 y 7. 23 ± 2.69 μg.mL - 1 para la cepa K75. Esas mismas cepas mostraron la mayor actividad total y específica de la enzima. Los resultados evidenciaron que no existe relación entre la producción de la enzima y la adsorción del colesterol. La cepa con mayor potenci al probiótico fue K73. La propiedad hipocolesterolémica de Lactobacillus fermentum , podría ser el inicio de la búsqueda de matrices alimentarias que permitan disminuir los niveles del colesterol sérico y adicionen valor agregado al producto.

  17. Uso de la amilasa termoestable de Bacillus licheniformis en la digestibilidad in vitro del almidón de sorgo y maíz

    OpenAIRE

    Rolando Rojo Rubio

    2001-01-01

    Varias enzimas celulolíticas se han usado para incrementar la digestion ruminal de la fibra y mejorar la producción en rumiantes. Sin embargo, se ha dado poca atención a las enzimas amilolíticas como un tratamiento a los granos, a pesar de que la productividad del ganado puede mejorar con el uso de mezclas de enzimas externas, incluyendo amilasas y celulasas. Así, se incubó sorgo, sorgo rolado con vapor y maíz (500 mg de grano), con fluido ruminal para estudiar el efecto de la adición de alfa...

  18. Monitoreo in vitro del potencial de cinco nutrientes (b, mn, zn, cu, si) sobre órganos de mycosphaerella fijiensis morelet agente causal de la sigatoka negra

    OpenAIRE

    Espinoza, Lisbeth Del Rocio; Jimenez, Maria Isabel

    2009-01-01

    La Sigatoka negra es una enfermedad que afecta a las hojas, es causada por Mycosphaerella fijiensis que es la principal limitante dentro del mercado de producción de bananas. Esta enfermedad es controlada a través de la aplicación aérea de pesticidas, pero la resistencia a estos es un problema que se ha ido incrementando. Como ha sido demostrado en algunas plantas el efecto inhibitorio de algunos micronutrientes en diferentes órganos. Se decidió investigar el efecto de diferentes concentracio...

  19. Estudio in vitro del ajuste marginal y la resistencia a fuerzas compresivas de coronas metal-cerámica de cromo-cobalto colado, sinterizado, mecanizado y presinterizado

    OpenAIRE

    Real Voltas, Francisco

    2015-01-01

    Cuando se realiza un tratamiento rehabilitador existen unas variables sobre las cuales se tiene cierto control (toma de impresiones, vaciado de las mismas, fabricación de la prótesis…) y otras que son totalmente incontrolables por parte del rehabilitador (hábitos alimentarios, parafunciones...). Esto hace que se busquen técnicas de trabajo que garanticen el éxito de los tratamientos, como mínimo, en aquellas variables que son controlables. El éxito de las prótesis fijas sobre dientes natu...

  20. Growth decline assessment in Pinus sylvestris L. and Pinus nigra Arnold. forest by using 3-PG model.

    Directory of Open Access Journals (Sweden)

    Rafael M. Navarro-Cerrillo

    2016-12-01

    Full Text Available Aim of the study: We assessed the ability of the 3-PG process-based model to accurately predict growth of Pinus sylvestris and P. nigra plantations across a range of sites, showing declining growth trends, in southern Spain. Area of study: The study area is located in “Sierra de Los Filabres” (Almería. Material and methods: The model was modified in fifteen parameters to predict diameter (DBH, cm, basal area increment (BAI, cm2 yr-1 and leaf area index (LAI, m2 m-2 in healthy trees and trees showing declining growth. We assumed that a set of specific physiological parameters (stem partitioning ratio-pFS20, maximum litterfall rate-γFx, maximum canopy conductance-gCx, specific leaf area for mature aged stands-σ1, age at which specific leaf area = ½ (σ0 + σ1, age at full canopy cover-tc, and canopy boundary layer conductance-gB included in 3-PG would be suitable for predicting growth decline related to climate conditions. The calibrated model was evaluated using dendrochronological and LAI data obtained from plots. Main results: Observed and simulated DBH showed a high correlation (R2 > 0.99 between modelled and measured values for both species. In contrast, modelled and observed BAI showed lower correlation (R2 < 0.68. Sensitivity analysis on 3-PG outputs showed that the foliage parameters - maximum litterfall rate, maximum canopy conductance, specific leaf area for mature aged stands, age at which specific leaf area, and age at full canopy cover - were important for DBH and BAI predictions under drought stress. Research highlights: Our overall results indicated that the 3-PG model could predict growth response of pine plantations to climatic stress with desirable accuracy in southern Spain by using readily available soil and climatic data with physiological parameters derived from experiments. Keywords: Hybrid process model; forest management models; growth prediction; Pinus spp, Parameterization; forest decline.

  1. Desarrollo de una metodología in vitro para estimar la tasa de fermentación de los forrajes en el intestino grueso del cerdo

    Directory of Open Access Journals (Sweden)

    Leterme Pascal

    2005-12-01

    Full Text Available La metodología para estimar la tasa de fermentación de los forrajes se desarrolló en dos etapas. La primera, de digestibilidad enzimática, simula la digestión ocurrida en el estómago e intestino delgado, mediante el empleo de pepsina y pancreatina; en esta se determina la digestibilidad de la materia seca. La segunda etapa de fermentación simula la fermentación que ocurre en el intestino grueso utilizando como inóculo heces de cerdo; en esta se determina la cantidad de gas producido durante la fermentación de los forrajes y la degradación de la fibra detergente neutra (FDN. Las dos etapas presentaron buena repetibilidad en el tiempo. Abstract Develop of an in vitro methodology to determine the forages fermentation in to the large intestine. The methodology was developing in two phases. The first phase (enzymatic digestibility simulates the digestion occurred in the stomach and small intestine, by means of the employment of pepsin and pancreatin; and the digestibility of the dry matter is determined. The second phase (in vitro fermentation simulates the fermentation that occurs in the large intestine utilizing pig faeces; the quantity of gas produced during the fermentation of the feedstuff is determined and determine the degradation of neutral detergent fiber (NDF. All the phases show a good repeatability throw the time. Key words: digestibility in vitro, pigs, fermentación, forages (Azolla filiculoides, Xanthosoma saggitifolium, Morus alba, Trichanthera gigantea y Salvinia molesta.

  2. Saturated salt method determination of hysteresis of Pinus sylvestris L. wood for 35 ºC isotherms

    Directory of Open Access Journals (Sweden)

    García Esteban, L.

    2004-12-01

    Full Text Available The saturated salts method was used in this study to quantify hysteresis in Pinus sylvestris L. wood, in an exercise that involved plotting the 35 ºC desorption and sorption isotherms. Nine salts were used, all of which establish stable and known relative humidity values when saturated in water The wood was kept at the relative humidity generated by each of these salts until the equilibrium moisture content (EMC was reached, both in the water loss or desorption, and the water uptake or sorption processes. The Guggenheim method was used to fit the values obtained to the respective curves. Hysteresis was evaluated in terms of the hysteresis coefficient, for which a mean value of 0.87 was found.

    Con este trabajo se ha cuantificado la histéresis de la madera de Pinus sylvestris L. Para ello, se han construido las isotermas de 35 ºC de adsorción y sorción, mediante el método de las sales saturadas. Se han utilizado nueve sales que cuando se saturan en agua dan lugar a unas humedades relativas estables y conocidas. La madera fue colocada bajo las distintas humedades relativas que confieren cada una de las sales hasta que alcanzaron las distintas humedades de equilibrio higroscópico, tanto en el proceso de pérdida de agua o desorción, como en el de adquisición de agua o de sorción. Los valores obtenidos fueron ajustados a las respectivas sigmoides, haciendo uso del método de Guggenheim. La valoración de la histéresis se determinó mediante el coeficiente de histéresis, obteniendo un valor medio de 0,87.

  3. Assessment of the variation of the moisture content in the Pinus pinaster Ait. using the non destructive GPR technique

    Directory of Open Access Journals (Sweden)

    Díez Barra, R.

    2011-03-01

    Full Text Available The moisture content variations in wood have a significant influence in wood’s physicochemical properties, as well as in its electromagnetic properties and to specific effects upon waves’ characteristics. In particular, this paper focuses on the analysis of the Ground-penetrating Radar’s (GPR using an antenna of 1.6GHz central frequency capacity to register the velocity and the amplitude of the electromagnetic waves’ variation during the drying process of Pinus pinaster Ait timber joists. The results showed that when timber MC descends, the propagation velocity and amplitude of both the direct and the reflected wave increased. The high correlation found between the variables studied demonstrates GPR efficiency and the innovative application of this technique as a non-destructive evaluation tool for timber structures, particularly when studying its moisture content.La variación en el contenido de humedad (CH tiene una influencia significativa tanto en las propiedades físico- químicas de la madera, como en sus propiedades electromagnéticas y por tanto afecta a las características de la propagación de las ondas. En concreto, en este trabajo se estudia la capacidad del georradar (GR empleando una antena de 1.6GHz de frecuencia central para registrar las variaciones que se producen en la velocidad y en la amplitud de las ondas electromagnéticas cuando se propagan en unas viguetas de madera de Pinus pinaster Ait de uso estructural cuyo CH va disminuyendo. Se ha comprobado como cuando el CH descendía la velocidad de propagación y las amplitudes, tanto de la onda directa como de la reflejada aumentaba. Los altos factores de correlación encontrados demuestran que el GR es una técnica capaz de evaluar, de forma no destructiva, el CH de la madera de uso estructural.

  4. Caracterización de algunas propiedades en tableros de corteza de Pinus pinaster Ait. y tableros de vermiculita expandida

    Directory of Open Access Journals (Sweden)

    Pereiro López, G.

    2012-06-01

    Full Text Available The search for new applications to use boards requires innovation of their pro-perties. The improvement of insulation properties, the possibility to regulate the environmental conditions, the improvement of fire resistance and the increase of physical and mechanical properties allow a more diverse use of boards. Using new components in the production of boards and/or component materials also called composites (1, enables the improvement of these properties. This publication analyses different physical and mechanical properties of various types of single-layer boards produced either based on expanded vermiculite particles bonded with soluble silicate glasses (2 or based on lignocelluloses particles bark of "Pinus pinaster" Ait., agglutinated with cement derivatives of magnesium (3, the latter included in the classification of the norm (4. Commercial wood wool boards (5 are used as reference material.La búsqueda de nuevas aplicaciones en el uso de los tableros requiere de la innovación en sus propiedades. Las mejoras en el aislamiento, en la regulación de las condiciones ambientales, en su resistencia al fuego y el incremento en sus propiedades físico-mecánicas, aumentan la diversificación de sus usos. El empleo de nuevas materias en los tableros y/o en los materiales compuestos, denominados composites (1, posibilita la mejora en estas propiedades. Esta publicación analiza diversas propiedades físicas y mecánicas de varios tipos de tableros monocapa, elaborados, por un lado, a base de partículas minerales de vermiculita expandida aglutinadas con silicatos de vidrios solubles (2, y por otro lado, a base de partículas lignocelulósicas de corteza de Pinus pinaster Ait., aglutinadas con cementos derivados del magnesio (3, incluidos ya estos últimos en la clasificación de la norma (4. Como material de referencia se emplean tableros comerciales de virutas de madera (5.

  5. Effects of ammonium and nitrate on mycorrhizal seedlings of Pinus sylvestris.

    NARCIS (Netherlands)

    Termorshuizen, A.J.; Ket, P.C.

    1991-01-01

    The effects of ammonium sulphate and sodium nitrate on Pinus sylvestris seedlings inoculated with Paxillus involutus and Suillus bovinus were investigated. Weekly fertilization (26 weeks) positively affected the plant dry weight, but inhibited the mycorrhizal formation and decreased the root

  6. EVALUACIÓN In Vitro DEL EFECTO DE FOSFOLÍPIDOS SOBRE LA GERMINACIÓN DE SEMILLAS DE TOMATE (Lycopersicon esculentum Mill)

    OpenAIRE

    María del C. Travieso; Oriela Pino; Yaíma Sánchez; Miriam Rojas; Belkis Peteira

    2015-01-01

    Se evalúo el efecto de una mezcla de fosfolípidos de origen natural, aplicados como sustrato en papel de filtro, sobre la germinación in vitro de semillas de tres cultivares de tomate (Amalia, Campbell 28 y Vita). En todos los casos se evidenció un efecto inductor sobre el crecimiento radicular de las plántulas de las semillas recién germinadas, dados por longitudes de radículas significativamente superiores en los grupos tratados con bajas concentraciones con respecto a los controles. Aunque...

  7. Optimización del método de recuperación de ovocitos para la fecundación in vitro

    OpenAIRE

    Rodríguez Zamora, Luis Arturo

    2013-01-01

    La producción de embriones in vitro es una de las biotecnologías con mayor potencial aplicativo en la producción bovina. Sin embargo, este proceso sólo permite obtener el 30–40% de blastocistos de los ovocitos utilizados, lo que demuestra ser poco eficiente para su empleo a escala comercial. Dentro de los múltiples factores causales, la cantidad y calidad de los ovocitos empleados representa uno de los principales elementos a tener en cuenta. Por ello, en el intento de optimiza...

  8. Ingenier??a del tejido cartilaginoso: obtenci??n de cart??lago "in vitro" para su utilizaci??n en ingenier??a de tejidos

    OpenAIRE

    Cola??o, Bruno Jorge Antunes

    2012-01-01

    317 p. Partiendo de la definici??n de la ingenier??a de tejidos como el arte de reconstruir tejidos, ya sea estructural o funcionalmente, el presente trabajo se centra en algunos de los tipos de c??lulas que se pueden utilizar para la producci??n de tejido in vitro. Los tipos de c??lulas elegidos para el estudio fueron: cuadrocitos auriculares de conejo, c??lulas troncales derivadas de tejido adiposo abdominal (CTDTA) e inguinal de rata y c??lulas troncales de tejido adiposo, a partir de g...

  9. Static and kinetic friction coefficients of Scots pine (Pinus sylvestris L., parallel and perpendicular to grain direction

    Directory of Open Access Journals (Sweden)

    Aira, J. R.

    2014-09-01

    Full Text Available In this study the static (µe and kinetic (µd coefficients of friction were obtained for Pinus sylvestis L. sawn timber of Spanish origin. Friction between transverse surfaces sliding perpendicular to the grain (tangential direction and radial surfaces sliding parallel to the grain was analyzed. A specifically designed device was used for tests, which makes it possible to apply contact pressure and measure displacements and applied loads simultaneously. Coefficients of friction between transverse surfaces (µe = 0,24; µd = 0,17 were about twice of the coefficients of friction between radial surfaces (µe = 0,12; µd = 0,08. Furthermore, these values are located within normal values of those commonly reported for softwood. The results are considered preliminary due to the small number of specimens.En este estudio se determinaron los coeficientes de rozamiento, estático (µe y dinámico (µd, en madera aserrada de Pinus sylvestris L. de procedencia española, diferenciando si se produce el contacto entre secciones de corte transversal con deslizamiento en dirección perpendicular a la fibra (en dirección tangencial, o entre secciones de corte radial con deslizamiento paralelo a la fibra. Para la realización de los ensayos se ha utilizado un dispositivo, diseñado específicamente, que posibilita la aplicación de una presión de contacto y la medición del desplazamiento y de la fuerza aplicada de manera simultánea, permitiendo la obtención de los coeficientes de rozamiento estático y dinámico. Los coeficientes de rozamiento obtenidos entre secciones transversales (µe = 0.24; µd = 0.17 fueron del orden del doble de los coeficientes de rozamiento entre secciones radiales (µe = 0.12; µd = 0.08. Además, estos valores se encuentran dentro de los valores que aparecen habitualmente en la bibliografía para madera de coníferas. Debido al escaso tamaño de la muestra los resultados se consideran preliminares.

  10. Síntesis in vitro de la proteína de la envoltura del virus peruano de la fiebre amarilla

    Directory of Open Access Journals (Sweden)

    Carlos Yábar V

    2001-01-01

    Full Text Available Objetivo: Sintetizar la proteína recombinante de la envoltura (Er del virus peruano de la Fiebre Amarilla (FA utilizando técnicas moleculares. Materiales y métodos: El gen de la proteína de interés fue amplificado por transcripción reversa - reacción en cadena de la polimerasa (RT-PCR y clonado en un vector plasmídico para ser analizado mediante secuenciamiento de ADN. El inserto de ADN fue subdonado en un vector de expresión para ser traducido a proteína. Se purificó la proteína mediante cromatografía de afinidad bajo condiciones denaturantes, siendo visualizada por electroforesis en SDS-PAGE. Resultados: Se sintetizó y purificó la pr E del virus de la FA. Presentó un peso de 66 kDa, y luego de tres horas de inducción a partir de 1x108 células (OD=0.5 se obtuvo 10 mg/mL de la proteína a miniescala. Conclusión: El análisis de la secuencia de aminoácidos demostró que Er podría ser un buen candidato a ser evaluado serológicamente y luego ser usado como herramienta de diagnóstico específico para la FA.

  11. Greek Pinus essential oils: larvicidal activity and repellency against Aedes albopictus (Diptera: Culicidae).

    Science.gov (United States)

    Koutsaviti, Katerina; Giatropoulos, Athanassios; Pitarokili, Danae; Papachristos, Dimitrios; Michaelakis, Antonios; Tzakou, Olga

    2015-02-01

    The needle volatiles metabolites of seven Pinus spp.: Pinus nigra (3 samples), Pinus stankewiczii, Pinus brutia, Pinus halepensis, Pinus canariensis, Pinus pinaster and Pinus strobus from Greece were determined by gas chromatography and gas chromatography-mass spectrometry. P. nigra and P. canariensis essential oils were dominated by α-pinene (24.9-28.9 % and 15 %, respectively) and germacrene D (20.3-31.9 % and 55.8 %, respectively), whereas P. brutia and P. strobus by α-pinene (20.6 % and 31.4 %, respectively) and β-pinene (31.7 % and 33.6 %, respectively). P. halepensis and P. pinaster oils were characterized by β-caryophyllene (28.5 % and 22.5 %, respectively). Finally, β-pinene (31.4 %), germacrene D (23.3 %) and α-pinene (17.5 %) were the most abundant compounds in the needle oil of P. stankewiczii. Additionally the larvicidal and repellent properties of their essential oils were evaluated against Aedes albopictus, a mosquito of great ecological and medical importance. The results of bioassays revealed that repellent abilities of the tested essential oils were more potent than their larvicidal activities. The essential oils of P. brutia, P. halepensis and P. stankewiczii presented considerable larvicidal activity (LC50 values 67.04 mgL(-1) and 70.21 mgL(-1), respectively), while the others were weak to inactive against larvae. The essential oils of P. halepensis, P. brutia, and P. stankewiczii presented a high repellent activity, even at the dose of 0.2 μL cm(-2), while in the dose of 0.4 μL cm(-2), almost all the tested EOs displayed protection against the mosquito.

  12. Production of laminated veneer lumber LVL using veneer of Schizolobium amazonicum, Eucalyptus saligna and Pinus taeda

    OpenAIRE

    Setsuo Iwakiri; Jorge Luis Monteiro de Matos; Juliana Afonso Pinto; Lívia Cássia Viana; Marina Moura de Souza; Rosilani Trianoski; Vanessa Coelho Almeida

    2010-01-01

    This research evaluated the quality of laminated veneer lumber - LVL manufactured with veneers of Schizolobium amazonicum (paricá), Eucalyptus saligna and Pinus taeda. The LVL panels were manufactured in the laboratory conditions composed by seven veneers, 2,0 mm thickness, with different structural compositions, using phenol-formaldehyde resin. The veneers of Schizolobium amazonicum- paricá- were pre-classified by using stress wave machine. The veneers of Eucalyptus saligna and Pinus taeda w...

  13. Familial variation in Pinus leiophylla Schiede ex Schltdl. & Cham. seedlings in response to drought: water and osmotic potential

    Directory of Open Access Journals (Sweden)

    Natalia Castelán-Muñoz

    2015-01-01

    Full Text Available La variación de plántulas de cuatro familias de Pinus leiophylla de diferentes procedencias se evaluó en respuesta a la sequía, considerando las variables potencial hídrico (Ψa, potencial osmótico (Ψ0, componentes y asignación de biomasa. Las familias de P. leiophylla se localizan en un huerto semillero del Colegio de Postgraduados en el Estado de México. El estudio se hizo con el fin de identificar los genotipos resistentes al estrés hídrico. Después de 26 días sin riego, 50 % de las plántulas presentaron decaimiento permanente del ápice del tallo, con Ψa = -3.35 MPa y Ψ0 = -3.23 MPa, lo que representó disminución de 596 y 112 %, respectivamente, por efecto de la sequía. La acumulación de biomasa también fue afectada significativamente (P = 0.05 en las familias evaluadas, a excepción de la procedente de San Rafael. En promedio, la biomasa de la raíz de las plántulas en sequía fue 38 % menor que las plántulas sin sequía. Las familias de P. leiophylla provenientes de San Juan Tetla y de Santa María Atepetzingo (ambas del estado de Puebla presentaron mejor respuesta al estrés impuesto, mientras que la familia de Tlalmanalco (Estado de México fue la más afectada.

  14. Gasificación con CO2 de carbonizados de aserrín de Pinus caribaea Morelet var. cariba

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    F. Márquez-Montesino

    2004-01-01

    Full Text Available Dada la importancia del estudio de la reactividad de los carbones con el fin de estudiar su activación, se desarrolló la gasificación con CO2 de un carbón obtenido de aserrín de Pinus caribaea. Los ensayos termogravimétricos se realizaron en un sistema modular de CI Electronics, las temperaturas de gasificación escogidas fueron 750, 775, 800 y 825 °C. Para calcular los valores de reactividad normalizada, se determinó el área de superficie por adsorción de CO2 a 273 K, en un equipo Autosorb-1 de Quantachrome Corporation sobre muestras desgasificadas a 180 °C y un vacío igual o mayor de 50 mtorr durante 8 h. De la gasificación con CO2 se representó gráficamente la reactividad frente a valores de conversión del carbonizado, indicando un aumento de la reactividad con el aumento de la temperatura de gasificación, lo que se explica por el desarrollo del área de superficie con el avance de la reacción y el efecto catalítico del mineral al aumentar su concentración en el sustrato. La representación gráfica de la ecuación de Arrhenius para la reactividad, indica que en las condiciones estudiadas en termobalanza, el proceso de gasificación ocurre bajo control de la etapa química, donde el valor de la energía de activación aparente es de ± 234 kJ/mol.

  15. Actividad antibacteriana, degradación hidrolítica y microbiana y citotoxicidad in vitro de poliuretanos sintetizados con polioles derivados del aceite de higuerilla

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    Yomaira L. Uscategui

    Full Text Available Biological activities from polyurethanes (PUs synthesized from polyols (derived from castor oil and isophorone diisocyanate were evaluated. In vitro degradability was evaluated by phosphate buffered saline (PBS and antibacterial degradation with Escherichia coli and Pseudomonas aeruginosa. The biocompatibility was analyzed by: i the antimicrobial activity against E. coli, S. aureus and P. aeruginosa, and ii in vitro cytotoxicity assays using mouse embryonic fibroblast cell line L-929 in direct contact with the PUs and with NIH/3T3 cells in indirect contact with the PUs degradation products. PUs with polyol P1 (2,64% molar relation of pentaerythritol and castor oil was the material with better biodegradation properties: 1.125±0.110% in 72 h by PBS at 100 ºC. In general, all materials were degraded up to 1.000±0.223% and 2.251±0.010% in 72 h by E. coli and P. aeruginosa, respectively. On the other hand, it was determined a close relationship between functionality of polyol and bacterial inhibition. The antibacterial effect of the PUs decreased by 67% for E. coli, 55% for S. aureus and 56% for P. aeruginosa after 24 h. Chemical modification of castor oil did not generate a cytotoxic effect on the tested cell lines. The evaluated materials are suggested as candidates to obtain biomaterials due to their mechanical properties and biocompatibility presented in untransformed cells.

  16. Propiedades antibacterianas in vitro de metabolitos secundarios aislados de dos especies del género Zanthoxylum (Rutaceae Antibacterial properties in vitro of secondary metabolites isolated from two species of Zanthoxylum (Rutaceae genus

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    Oscar Javier Patiño Ladino

    2011-09-01

    Full Text Available Actualmente las enfermedades infecciosas originadas por diferentes microorganismos, son una de las mayores causas de muerte a nivel mundial, razón por la que la búsqueda de nuevos agentes antimicrobianos se ha hecho indispensable, y los productos naturales han constituido una de las fuentes potenciales para encontrar compuestos antimicrobianos. Las especies del género Zanthoxylum se caracterizan por presentar diversos tipos de metabolitos secundarios, muchos de los cuales han mostrado interesantes propiedades biológicas contra diversos microorganismos patógenos humanos. El objetivo de esta investigación consistió en evaluar la actividad antibacteriana de 21 sustancias aisladas de 2 especies del género Zanthoxylum (Rutaceae. La actividad fue determinada por el método de difusión radial en agar contra cepas de bacterias estándar grampositivas: Staphylococcus aureus ATCC 6538 y Enterococcus faecalis ATCC 29212 y gramnegativas: Escherichia coli ATCC 25922 y Salmonella tiphymurium, cepas ATCC 14028s y MS7953. De las 21 sustancias naturales evaluadas, 11 causaron algún grado de inhibición del crecimiento bacteriano. El alcaloide benzofenantridínico queleritrina presentó la mayor actividad, e inhibió el crecimiento de las 5 cepas con halos de inhibición comparables o superiores a los mostrados por los antibióticos empleados como controles positivos. Los alcaloides isoquinolínicos en general, son las sustancias más promisorias para continuar con estudios más específicos de actividad antibacteriana, pues además de presentar una actividad interesante, algunas de estas sustancias inhiben tanto el crecimiento de bacterias grampositivas como el de bacterias gramnegativas, lo cual indica que son sustancias que presentan actividad antibacteriana de amplio espectro.Currently, infectious diseases caused by different microorganisms are one of major causes of death worldwide, so the search for new antimicrobial agents has become

  17. Genetic variation of F1 hybrids from controlled crosses between Pinus montana var. rostrata and Pinus sylvestris in anatomical needle traits

    Directory of Open Access Journals (Sweden)

    Maria A. Bobowicz

    2014-01-01

    Full Text Available One hundred and seventeen individuals representing ten hybrid families from controlled crosses between Pinus montana var. rostrata and Pinus sylvestris were examined with respect to eight traits of anatomy of their two-year old needles. The biometric data obtained by measurements of anatomical traits were used to conduct multivariant statistical analyses with discriminant analysis and Mahalanobis distances as principal methods. Some of the hybrid families were similar to parental species representing the typ Montana or Sylvestris, and some less numerous intermediate forms. The results of the anatomical studies correspond to a great extent to those obtained for isoenzymes and antigene proteins (Prus-Głowacki and Stephan 1998.

  18. Patrones iniciales de sucesión en claros experimentales de plantaciones de Pinus patula en el Embalse de Chisacá, Usme, Cundinamarca (Colombia

    Directory of Open Access Journals (Sweden)

    Vargas Ríos Orlando

    2006-06-01

    Full Text Available Se presenta un estudio que determina el efecto de la creación de claros experimentales con diferentes densidades sobre los patrones iniciales de sucesión dentro de plantaciones de Pinus patula. Adicionalmente, se evaluó el efecto de la remoción de acículas del suelo. Antes del establecimiento de los claros ya se encontraban en las parcelas la mayoría de las especies (30, pero en muy baja proporción; luego del aclareo, se registraron
    25 especies determinadas entre plantas vasculares (20 y no vasculares (5; 13 de éstas resultaron de mayor relevancia debido a su mayor cobertura en las parcelas. Se pudo establecer que la apertura de claros, además de la mayor disponibilidad de luz solar, permite una mayor entrada de agua, con lo cual se estimula la germinación, crecimiento y establecimiento de individuos relativamente rápido. Se determinó que existe un banco de semillas dentro de las acículas del suelo. Es importante continuar con el monitoreo de las parcelas para un mejor entendimiento del fenómeno sucesional que ocurre.

  19. RECONSTRUCCIÓN DE PRECIPITACIÓN INVIERNO-PRIMAVERA CON ANILLOS ANUALES DE Pinus douglasiana EN LA RESERVA DE LA BIOSFERA SIERRA DE MANANTLÁN, JALISCO

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    Julián Cerano-Paredes

    2013-01-01

    Full Text Available Se reconstruyó la precipitación invierno-primavera de 219 años (1792-2010 de la Reserva de la Biosfera Sierra de Manantlán (RBSM, Jalisco, México, empleando los anillos anuales de Pinus douglasiana (Mtz. como “proxy”. La sequía de mediados del siglo XX (1941-1963 fue la más severa del periodo estudiado. El índice multivariado de El Niño Oscilación del Sur (ENSO; 1959-2010 y el índice de ancho del anillo de P. douglasiana mostraron correlación significativa (r = 0.4542; P < 0.05 de los meses de enero-junio, con mayor correlación en los meses de enero (r = 0.4337; P < 0.0001 y marzo (r = 0.4310; P < 0.0001. Las teleconexiones de ENSO en la región varían a través del tiempo; de enero a junio, los eventos El Niño están asociados con precipitaciones mayores que el promedio, mientras que los eventos La Niña muestran lo contrario.

  20. Essential Oil of Pinus koraiensis Exerts Antiobesic and Hypolipidemic Activity via Inhibition of Peroxisome Proliferator-Activated Receptors Gamma Signaling

    Directory of Open Access Journals (Sweden)

    Hyun-Suk Ko

    2013-01-01

    Full Text Available Our group previously reported that essential oil of Pinus koraiensis (EOPK exerts antihyperlipidemic effects via upregulation of low-density lipoprotein receptor and inhibition of acyl-coenzyme A. In the present study, we investigated the antiobesity and hypolipidemic mechanism of EOPK using in vitro 3T3-L1 cells and in vivo HFD-fed rats. EOPK markedly suppressed fat accumulation and intracellular triglyceride associated with downregulation of adipogenic transcription factor expression, including PPARγ and CEBPα in the differentiated 3T3-L1 adipocytes. Additionally, EOPK attenuated the expression levels of FABP and GPDH as target genes of PPARγ during adipocyte differentiation. Furthermore, PPARγ inhibitor GW9662 enhanced the decreased expression of FABP and PPARγ and fat accumulation induced by EOPK. To confirm the in vitro activity of EOPK, animal study was performed by administering normal diet, HFD, and/or EOPK at the dose of 100 or 200 mg/kg for 6 weeks. Consistently, EOPK significantly suppressed body weight gain, serum triglyceride, total cholesterol, LDL cholesterol, and AI value and increased HDL cholesterol in a dose-dependent manner. Immunohistochemistry revealed that EOPK treatment abrogated the expression of PPARγ in the liver tissue sections of EOPK-treated rats. Taken together, our findings suggest that EOPK has the antiobesic and hypolipidemic potential via inhibition of PPARγ-related signaling.

  1. Control of Diplodia pinea and D. scrobiculata in Pinus halepensis by 5-chloro-salicylic acid

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    A. Moret

    2007-08-01

    Full Text Available Diplodia pinea (syn. Sphaeropsis sapinea and D. scrobiculata are destructive pathogens of conifer species in many parts of the world. The sensitivity of these fungi to externally applied 5-chloro-salicylic acid on Pinus halepensis was studied. Trees treated with 2 mM 5-chloro-salicylic acid were more resistant to the fungi than untreated trees. After 15 days of treatment shoot dieback affected 30% of trees inoculated with D. pinea, compared to 60% of untreated trees. D. scrobiculata caused shoot dieback in 30% of untreated trees but only in 20% of trees pretreated with 5-chloro-salicylic acid. The controls never developed tip blight. The direct effect of 5-chloro-salicylic acid on the mycelial growth of D. pinea and D. scrobiculata was tested in vitro using PDA amended with 5-chloro-salicylic acid at five concentrations (0.2, 1, 2, 2.5, and 3.0 mM. The radial growth of colonies was measured after 48 and 72 h of incubation at 24°C. After 48 h, 5-chloro-salicylic acid significantly inhibited mycelial growth of D. pinea at 3mM, although there was no longer any significant difference in growth rates after 72 h of incubation. D. scrobiculata was slightly more sensitive to 5-chloro-salicylic acid than D. pinea. After 48 h, significant differences were observed in the mean colony diameter of D. scrobiculata when directly exposed to 5-chloro-salicylic acid at dilutions from 0 to 3 mM. After 72 h, however, mycelial growth was reduced significantly only at the highest concentrations (2; 2.5 and 3 mM (P-value <0.05.

  2. Strategies utilized by trophically diverse fungal species for Pinus sylvestris root colonization.

    Science.gov (United States)

    Mucha, Joanna; Guzicka, Marzenna; Ratajczak, Ewelina; Zadworny, Marcin

    2014-01-01

    Physiological changes in host plants in response to the broad spectrum of fungal modes of infection are still not well understood. The current study was conducted to better understand the infection of in vitro cultures of Pinus sylvestris L. seedlings by three trophically diverse fungal species, Fusarium oxysporum E. F. Sm. & Swingle, Trichoderma harzianum Rifai and Hebeloma crustuliniforme (Bull.) Quél. Biochemical methods and microscopy were utilized to determine (i) which factors (apoplastic and cellular pH, reactive oxygen species, glutathione and cell death) play a role in the establishment of pathogenic, saprotrophic and mycorrhizal fungi, and (ii) whether cell death is a common response of conifer seedling tissues when they are exposed to trophically diverse fungi. Establishment of the pathogen, F. oxysporum, was observed more frequently in the meristematic region of root tips than in the elongation zone, which was in contrast to T. harzianum and H. crustuliniforme. Ectomycorrhizal (ECM) hyphae, however, were occasionally observed in the studied root zone and caused small changes in the studied factors. Colonization of the meristematic zone occurred due to host cell death. Independently of the zone, changes in cellular pH resulting in an acidic cytoplasm conditioned the establishment of F. oxysporum. Additionally, cell death was negatively correlated with hydrogen peroxide (H2O2) in roots challenged by a pathogenic fungus. Cell death was the only factor uniquely associated with the colonization of host roots by a saprotrophic fungus. The mechanism may differ, however, between the zones since apoplastic pH was negatively correlated with cell death in the elongation zone, whereas in the meristematic zone, none of the studied factors explained cell death. Colonization by the ECM fungus, H. crustuliniforme, was associated with a decreasing number of cells with acidic apoplast and by production of H2O2 in the elongation zone resulting in cell death. Saprotrophic

  3. Estudio in vitro del efecto erosivo en la superficie del esmalte dentario, por acción de tres bebidas artificiales no alcohólicas, valorado a través de la microdureza adamantina

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    Fernando Ramiro Guanoluisa

    2014-12-01

    Full Text Available Uno de los problemas que los odontólogos atienden cada vez con mayor frecuencia son los desgastes dentales, que incluyen a la erosión, caracterizada por la pérdida crónica de la estructura mineral del diente por acción de ácidos no bacterianos. Se comparó el efecto erosivo en la superficie de esmalte dentario por acción de tres bebidas de alto consumo en la ciudad de Quito, a través de la valoración de la microdureza adamantina inicial y final, con el empleo del microdurómetro Rockwell. Para lo cual se utilizaron 65 bloques de esmalte con una dureza inicial entre, 320 y 366 Kg/mm2 según la escala Knoop. Los cuales fueron divididos en 32 y 33 elementos, para dos protocolos erosivos (con y sin saliva natural. Las bebidas empleadas fueron: Coca-Cola, Gatorade y V220; cada grupo fue sometido 10 minutos diarios por un período de 5 días a la acción de cada bebida (siendo omitido este paso en los grupos control. Al sexto día, se midió nuevamente la microdureza para determinar el objetivo propuesto. Los resultados fueron analizados con ANOVA, prueba Tukey y Prueba-T. Se concluyó que al aplicar los dos protocolos, hubo una disminución significativa de la microdureza del esmalte, siendo mayor en el que no se incluyó la saliva, donde sobresale la Coca-Cola (pH: 2,64 ya que la erosión guardó relación inversa con el pH de la bebida (a menor pH, mayor erosión. En cambio, cuando el esmalte estuvo expuesto a saliva, la erosión guardó relación con la composición y efervescencia de los productos, resultando ser el V220, el que presentó mayor erosión.

  4. Efecto in vitro del extracto acuoso de Dichrostachys cinerea (L. Wight & Arn. en el desarrollo de las fases exógenas de estrongílidos gastrointestinales de ovinos In vitro effect of the aqueous extract of Dichrostachys cinerea (L. Wight &Arn. on the development of exogenous stages of gastrointestinal strongyles in sheep

    Directory of Open Access Journals (Sweden)

    J Arece

    2012-09-01

    Full Text Available Se evaluó el efecto in vitro de extractos acuosos de hojas de marabú (Dichrostachys cinerea en la eclosión de huevecillos, el desarrollo larvario y la migración de las larvas del tercer estadio de estrongílidos gastrointestinales. Los tratamientos fueron tres concentraciones de extractos acuosos de hojas de marabú (500, 250 y 125 mg/mL, soluciones de albendazol y levamisol, así como el Phosphate Buffer Saline (PBS y el dimetilsulfóxido (DMSO como controles; el diseño fue completamente aleatorizado. Los porcentajes de eclosión presentaron diferencias significativas entre postratamientos. El PBS ocasionó la mayor tasa de eclosión (96,68%; el 30,51% eclosionó con el albendazol y los extractos de hojas de marabú mostraron tasas de eclosión moderadas (entre 49,75 y 66,54%, al parecer dependientes de la dosis. El desarrollo de las larvas L1/L2 a las L3 tratadas con extracto acuoso de marabú también presentó efectos dosis-dependientes y diferencias significativas con respecto a los grupos controles positivos y negativos. La dosis de 500 mg/mL inhibió el desarrollo larvario en porcentajes similares a los del albendazol (8,89 y 1,28%, respectivamente. El medio PBS no interfirió en la capacidad de migración de las larvas, mientras que con el levamisol los valores se redujeron de 86,45 a 93,92%; los extractos acuosos también redujeron significativamente dicha migración, con valores entre 77,67 y 48,97%. Los extractos acuosos de D. cinerea presentaron actividad antihelmíntica in vitro en los tres estadios de desarrollo del ciclo exógeno de los estrongílidos gastrointestinales de ovinos; esta actividad resultó más evidente en el desarrollo y la migración de las larvas de estos nemátodos.The in vitro effect of aqueous extract of leaves from Dichrostachys cinerea on egg hatching, larval development and migration of third-stage larvae of gastrointestinal strongyles, was evaluated. The treatments were three concentrations of aqueous

  5. Efecto in vitro de aceites esenciales de tres especies de Lippia sobre Moniliophthora roreri (Cif. y Par. Evans et al., agente causante de la moniliasis del cacao (Theobroma cacao L.

    Directory of Open Access Journals (Sweden)

    Betty Stefany Lozada

    2012-04-01

    Full Text Available Se evaluó el efecto antifúngico in vitro de cinco aceites esenciales (AEs (AE1, AE2, AE3, AE4 y AE5 extraídos de Lippia origanoides, L. citriodora y L. alba sobre aislados de monilia (Moniliophthora roreri obtenidos de frutos de cacao infectados provenientes de San Vicente de Chucurí, Santander, Colombia. Las plantas de Lippia fueron colectadas en cinco localidades colombianas. Los aislados de monilia (M1, M2, M3, M4 y M5 fueron caracterizados por su morfología, germinación y crecimiento en medios de cultivo. La actividad antifúngica de diferentes concentraciones de los AEs fue evaluada contra el aislado M2 y la cepa de M. roreri (ATCC 64239, para determinar su efecto sobre la germinación y la inhibición del crecimiento micelial. Los AEs estudiados inhibieron 100% de la germinación y del crecimiento micelial cuando fueron utilizados en concentraciones de 800 - 1000 µg/ml. Concentraciones de 200 µg/ml también mostraron efecto sobre los aislamientos fúngicos, siendo los AEs obtenidos de L. origanoides (AE2 y AE3 los más activos. Estos estaban compuestos principalmente por timol, p-cimeno, g-terpineno, acetato de timilo, carvacrol, b-mirceno, trans-b-cariofileno. Diferencias significativas (P < 0.05 sobre la susceptibilidad se observaron entre las dos cepas fúngicas estudiadas, siendo en general más susceptible el aislado M2 que la cepa ATCC. Los AEs de L. origanoides son candidatos para ser usados como posibles biofungicidas en el control de la moniliasis. Son necesarios estudios futuros orientados a determinar la actividad in vivo antifúngica de estos AEs y sus principales componentes.

  6. Rendimiento de aceite esencial en Pinus caribaea MorElet según el secado al sol y a la sombra. III

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    Rolando Quert Álvarez

    2001-04-01

    Full Text Available Se presentan los resultados obtenidos en la determinación del rendimiento de aceite esencial Pinus caribaea Moralet (pino macho, en función de las condiciones y tiempo de exposición al sol y a la sombra del follaje de dicha especie endémica de Cuba. El estudio se realizó con árboles existentes en áreas de la Estación Experimental Forestal de Viñales, provincia de Pinar del Río con edad de 30 a. El tamaño de muestra fue de 3 árboles y el tiempo de destilación para la extracción del aceite esencial de 3 h. Se tomaron muestra del follaje entre 1 y 20 d expuesto a las condiciones de secado, en intervalos de 1, 3, 6, 10 y 20 d. Los resultados obtenidos demostraron que el follaje expuesto a la sombra contiene un mayor porcentaje de aceite esencial que el follaje expuesto al sol, y que el tiempo de exposición al sol, influye significativamente sobre el rendimiento a partir del tercer día, mientras que en el follaje expuesto a la sombra, las diferencias se hacen significativas a partir de los 6 d.The results obtained in the determination of the yield of the essential oil from Pinus caribaea Moralet (male pine according to the conditions and time of exposure to the sun and to the shade of the foliage of this Cuban endemic species are presented. Trees of 30 years old located in the areas of the Experimental Forestal Station of Viñales, Pinar del Río province, were used in the study. The size of the sample was of 3 trees and the time of distillation for the extraction of the essential oil was of 3 hours. Specimens of the foliage were taken at intervals of 1, 3, 6, 10 and 20 days. The results obtained showed that the foliage exposed to the shade has a higher percentage of essential oil that the foliage exposed to the sun, and that the time of exposure to the sun influences significantly on the yield starting from the third day on, whereas in the foliage exposed to the shade the differences are remarkable from the sixth day on.

  7. Detección de Chlamydia pneumoniae en tejido aórtico humano: amplificación del gen kdtA e hibridación in vitro.

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    Natalia Guzmán

    2005-12-01

    Full Text Available Introducción. La ateroesclerosis es la principal causa de enfermedad coronaria y cerebrovascular, las cuales, a su vez, son las causas más comunes de mortalidad y morbilidad en el mundo occidental. Publicaciones recientes sugieren que ciertos microorganismos infecciosos podrían jugar un papel importante en la génesis y progresión de la aterosclerosis. De acuerdo con reportes seroepidemiológicos y de detección directa, Chlamydia pneumoniae podría ser el candidato más plausible. No obstante, no se ha determinado su papel específico en el proceso aterogénico, por lo cual en los últimos años ha surgido la necesidad de explorar diversas técnicas de detección de C. pneumoniae en arterias. Objetivo. El propósito de este estudio fue investigar la presencia de C. pneumoniae en muestras de tejido aórtico de catorce pacientes sometidos a cirugía de reemplazo aórtico, utilizando la amplificación del gen kdtA por PCR acoplada a un ensayo de hibridación in vitro. Materiales y métodos. De cada uno de catorce segmentos de aorta se obtuvo una muestra al azar para la extracción de ADN y la detección de C. pneumoniae por PCR-hibridación in vitro. Resultados. Doce (85,7% de catorce muestras de tejido de aorta resultaron positivas para C. pneumoniae. Conclusión. Los resultados encontrados en este estudio sugieren que la presencia de C. pneumoniae es frecuente en el tipo de muestras analizado. En estudios posteriores resultaría importante examinar si esta proporción se mantiene en una muestra poblacional mayor.

  8. [Soil microbial functional diversity of different altitude Pinus koraiensis forests].

    Science.gov (United States)

    Han, Dong-xue; Wang, Ning; Wang, Nan-nan; Sun, Xue; Feng, Fu-juan

    2015-12-01

    In order to comprehensively understand the soil microbial carbon utilization characteristics of Pinus koraiensis forests, we took the topsoil (0-5 cm and 5-10 cm) along the 700-1100 m altitude in Changbai Mountains and analyzed the vertical distributed characteristics and variation of microbial functional diversity along the elevation gradient by Biolog microplate method. The results showed that there were significant differences in functional diversity of microbial communities at different elevations. AWCD increased with the extension of incubation time and AWCD at the same soil depth gradually decreased along with increasing altitude; Shannon, Simpson and McIntosh diversity index also showed the same trend with AWCD and three different diversity indices were significantly different along the elevation gradient; Species diversity and functional diversity showed the same variation. The utilization intensities of six categories carbon sources had differences while amino acids were constantly the most dominant carbon source. Principal component analysis (PCA) identified that soil microbial carbon utilization at different altitudes had obvious spatial differentiation, as reflected in the use of carbohydrates, amino acids and carboxylic acids. In addition, the cluster of the microbial diversity indexes and AWCD values of different altitudes showed that the composition of vegetation had a significant impact on soil microbial composition and functional activity.

  9. Impacts of urban levels of ozone on Pinus halepensis foliage.

    Science.gov (United States)

    Soda; Bussotti; Grossoni; Barnes; Mori; Tani

    2000-08-01

    Between May and September, 1996, seedlings of Pinus halepensis were placed at a site adjacent to an automated air pollution monitoring station within the urban area of Florence. Additional 'control' plants were placed in chambers ventilated with charcoal/Purafil(R)-filtered air. All trees were well watered throughout the whole experimental period. During the exposure period, ambient levels of sulphur dioxide were very low, whilst the accumulated hourly exposure to ozone above 40 ppb (i.e. AOT40) exceeded 20000 ppb h(-1) - peak hourly ozone concentrations rising to levels above 100 ppb. Trees exposed to ambient levels of air pollution exhibited typical symptoms of ozone damage (chlorotic mottle) on previous year needles toward the end of the summer. Similar symptoms were not observed on equivalent trees exposed to filtered-air, nor were visible symptoms accompanied by insect or pest infestation. Anatomical and ultrastructural observations made on symptomatic needles revealed degeneration in mesophyll cells bordering sub-stomatal cavities and alterations in chloroplast ultrastructure (fat accumulation, starch and tannin pattern modifications). These observations are consistent with the known effects of air pollutants (namely ozone) recorded in the literature. Findings are discussed in relation to the impacts of ozone on P. halepensis in the Mediterranean region.

  10. Hydraulic adjustments underlying drought resistance of Pinus halepensis.

    Science.gov (United States)

    Klein, Tamir; Cohen, Shabtai; Yakir, Dan

    2011-06-01

    Drought-induced tree mortality has increased over the last decades in forests around the globe. Our objective was to investigate under controlled conditions the hydraulic adjustments underlying the observed ability of Pinus halepensis to survive seasonal drought under semi-arid conditions. One hundred 18-month saplings were exposed in the greenhouse to 10 different drought treatments, simulating combinations of intensities (fraction of water supply relative to control) and durations (period with no water supply) for 30 weeks. Stomata closed at a leaf water potential (Ψ(l)) of -2.8 MPa, suggesting isohydric stomatal regulation. In trees under extreme drought treatments, stomatal closure reduced CO(2) uptake to -1 µmol m(-2) s(-1), indicating the development of carbon starvation. A narrow hydraulic safety margin of 0.3 MPa (from stomatal closure to 50% loss of hydraulic conductivity) was observed, indicating a strategy of maximization of CO2 uptake in trees otherwise adapted to water stress. A differential effect of drought intensity and duration was observed, and was explained by a strong dependence of the water stress effect on the ratio of transpiration to evapotranspiration T/ET and the larger partitioning to transpiration associated with larger irrigation doses. Under intense or prolonged drought, the root system became the main target for biomass accumulation, taking up to 100% of the added biomass, while the stem tissue biomass decreased, associated with up to 60% reduction in xylem volume.

  11. Composition and chemical variability of Corsican Pinus halepensis cone oil.

    Science.gov (United States)

    Nam, Anne-Marie; Casanova, Joseph; Tomi, Félix; Bighelli, Ange

    2014-09-01

    The composition of the essential oil isolated from cones of Pinus halepensis grown in Corsica has been investigated by a combination of chromatographic (CC, GC) and spectroscopic (MS, 13C NMR) techniques. In total, 48 compounds that accounted for 95.5% of the whole composition have been identified. α-Pinene (47.5%) was the major component followed by myrcene (11.0%), (E)-β-caryophyllene (8.3%) and caryophyllene oxide (5.9%). Various diterpenes have been identified by 13C NMR in the fractions of CC. Fifteen oil samples isolated from cones harvested in three forests have been analyzed and two groups of similar importance have been differentiated within the 15 compositions. Oil samples of the first group contained α-pinene (mean 45.0 g/100 g, SD = 5.5) as the major component. The composition of samples of the second group was dominated by myrcene (mean 30.3 g/100g, SD = 9.0) and α-pinene (mean 24.6 g/100 g, SD = 3.1).

  12. Nitrogen metabolism in Lignifying Pinus taeda cell cultures

    Science.gov (United States)

    van Heerden, P. S.; Towers, G. H.; Lewis, N. G.

    1996-01-01

    The primary metabolic fate of phyenylalanine, following its deamination in plants, is conscription of its carbon skeleton for lignin, suberin, flavonoid, and related metabolite formation. Since this accounts for approximately 30-40% of all organic carbon, an effective means of recycling the liberated ammonium ion must be operative. In order to establish how this occurs, the uptake and metabolism of various 15N-labeled precursors (15N-Phe, 15NH4Cl, 15N-Gln, and 15N-Glu) in lignifying Pinus taeda cell cultures was investigated, using a combination of high performance liquid chromatography, 15N NMR, and gas chromatograph-mass spectrometry analyses. It was found that the ammonium ion released during active phenylpropanoid metabolism was not made available for general amino acid/protein synthesis. Rather it was rapidly recycled back to regenerate phenylalanine, thereby providing an effective means of maintaining active phenylpropanoid metabolism with no additional nitrogen requirement. These results strongly suggest that, in lignifying cells, ammonium ion reassimilation is tightly compartmentalized.

  13. Pharmaceutical and nutraceutical effects of Pinus pinaster bark extract

    Science.gov (United States)

    Iravani, S.; Zolfaghari, B.

    2011-01-01

    In everyday life, our body generates free radicals and other reactive oxygen species which are derived either from the endogenous metabolic processes (within the body) or from external sources. Many clinical and pharmacological studies suggest that natural antioxidants can prevent oxidative damage. Among the natural antioxidant products, Pycnogenol® (French Pinus pinaster bark extract) has been received considerable attention because of its strong free radical-scavenging activity against reactive oxygen and nitrogen species. P. pinaster bark extract (PBE) contains polyphenolic compounds (these compounds consist of catechin, taxifolin, procyanidins of various chain lengths formed by catechin and epicatechin units, and phenolic acids) capable of producing diverse potentially protective effects against chronic and degenerative diseases. This herbal medication has been reported to have cardiovascular benefits, such as vasorelaxant activity, angiotensin-converting enzyme inhibiting activity, and the ability to enhance the microcirculation by increasing capillary permeability. Moreover, effects on the immune system and modulation of nitrogen monoxide metabolism have been reported. This article provides a brief overview of clinical studies describing the beneficial and health-promoting effects of PBE. PMID:22049273

  14. Dynamics of monoterpene emissions in Pinus sylvestris during early spring

    Energy Technology Data Exchange (ETDEWEB)

    Baeck, J.; Hari, P.; Juurola, E. [Department of Forest Ecology, University of Helsinki (Finland); Hakola, H. [Finnish Meteorological Institute, Research and Development, Helsinki (Finland); Kulmala, M. [Department of Physical Sciences, University of Helsinki (Finland)

    2005-07-01

    The seasonal dynamics of biogenic volatile organic compound (BVOC) emissions, which can be related to the formation and growth of secondary organic aerosols, represent an important but at the present poorly understood linkage between vegetation activity and climate. Although a close relationship between photosynthesis and terpenoid emissions has been proposed, high monoterpene emission rates for Scots pine shoots (Pinus sylvestris) are frequently recorded during spring, in times when photosynthetic activity is strongly inhibited due to inherent seasonal restrictions. We suggest that terpenoid emissions are related to either photosynthesis or photorespiration for precursors for terpenoid biosynthesis. We developed two dynamic models describing temporal fluctuations in Scots pine monoterpene emissions, calculating the emissions by using CO{sub 2} exchange and ambient climate data. The models accurately predicted the measured monoterpene flux, and especially in March-April, during the time when photosynthesis was negligible and ambient temperatures were between -5 and +15 C, a good agreement was found with measured emissions and the model involving photorespiration. (orig.)

  15. Aboveground Tree Biomass for Pinus ponderosa in Northeastern California

    Directory of Open Access Journals (Sweden)

    Todd A. Hamilton

    2013-03-01

    Full Text Available Forest managers need accurate biomass equations to plan thinning for fuel reduction or energy production. Estimates of carbon sequestration also rely upon such equations. The current allometric equations for ponderosa pine (Pinus ponderosa commonly employed for California forests were developed elsewhere, and are often applied without consideration potential for spatial or temporal variability. Individual-tree aboveground biomass allometric equations are presented from an analysis of 79 felled trees from four separate management units at Blacks Mountain Experimental Forest: one unthinned and three separate thinned units. A simultaneous set of allometric equations for foliage, branch and bole biomass were developed as well as branch-level equations for wood and foliage. Foliage biomass relationships varied substantially between units while branch and bole biomass estimates were more stable across a range of stand conditions. Trees of a given breast height diameter and crown ratio in thinned stands had more foliage biomass, but slightly less branch biomass than those in an unthinned stand. The observed variability in biomass relationships within Blacks Mountain Experimental Forest suggests that users should consider how well the data used to develop a selected model relate to the conditions in any given application.

  16. MICROBIOTA OF PINUS POLLEN AS ADJUVANT FACTOR OF ALLERGY

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    Tetiana Shevtsova

    2016-06-01

    Full Text Available Bacteria, their endotoxin and mold found on pollen can be a reason of respiratory symptoms in sensitized individuals. This question concerns an anemophilous pollen more acute. In this work quantitative by dilution plating method and qualitative microbial analysis by MALDI-TOF MS Biotyper of pollen and other plants organs of Pinus sylvestris L., P. nigra Arnold, P. mugo Turra, P. armandii Franch., P. wallichiana A.B. Jacks from Nitra, Slovakia are performed which shows quantitative and species differences in mesophilic aerobic (0.00-6.27 log cfu/g and anaerobic bacteria (0.00-3.70 log cfu/g, enterococci (0.00 log cfu/g, coliform bacteria (0.00-5.29 log cfu/g, lactobacilli (0.00-4.20 log cfu/g, microscopic fungi and yeasts (2.60-5.29 log cfu/g content. Representatives of Pseudomonas (14, Bacillus (2, Acinetobacter (1, Arthrobacter (1, Pantoea (1, Klebsiella (1, Penicillium (6, Aspergillus (4, Cladosporium (1, Debaryomyces (1 genera were revealed on pine trees. The allergenic potential of the identified association of microorganisms on pollen has been evaluated based on published data. The results may be useful for aerobiologists, allergists and microbiologists, at least at the local level.

  17. Genetic diversity and the mating system of a rare Mexican Piñon, Pinus pinceana, and a comparison with Pinus maximartinezii (Pinaceae)

    Science.gov (United States)

    F. Thomas Ledig; Miguel A. Capó-Arteaga; Paul D. Hodgskiss; Hassan Sbay; Celestino Flores-López; M. Thompson Conkle; Basilio Bermejo-Velázquez

    2001-01-01

    Weeping piñon (Pinus pinceana) has a restricted and fragmented range, trees are widely scattered within populations, and reproduction is limited. Nevertheless, genetic diversity was high; based on 27 isozyme loci in 18 enzyme systems, unbiased expected heterozygosity averaged 0.174. Differentiation also was high (FST = 0.152),...

  18. EVALUATION OF THE GROWTH FACTOR IGF-I IN THE IN VITRO MATURATION OF CANINE OOCYTES IN THE TCM 199 MEDIUM Evaluación del factor de crecimiento IGF-I en la maduración in vitro de ovocitos de perras en el medio TCM 199 AVALIAÇÃO DO FATOR DE CRESCIMENTO IGF-I NA MATURAÇÃO IN VITRO DE OÓCITOS CANINOS NO MEIO TCM 199

    Directory of Open Access Journals (Sweden)

    Maricy Apparício Ferreira

    2009-07-01

    Full Text Available The aim was to evaluate the supplementation of recombinant IGF-I growth factor at concentration of 100?g/mL in 500µL of TCM 199 medium in the in vitro maturation of canine oocytes. The oocytes were stained with isotiocianate of fluorescein (Lens cullinaris and Hoeschst 33342 for nuclear and cytoplasmatic assessment using an epifluorescence microscope (filter FITC: excitement 490nm and emission 520nm. There were no statistical differences between control and supplemented groups regarding cytoplasmatic and nuclear maturation when using Kruskal-Wallis test, p<0,05.

    Key words: Canine, IGF-I, maturation, oocyte.
    El estudio presente tuvo como objetivo principal evaluar la adición del factor de crecimiento IGF-I recombinante en la concentración de 100hg/mL en 500mL del medio TCM 199 en la maduración in vitro de ovocitos de perras. Se evaluó con eso el grado de maduración citoplasmático y nuclear por medio de tinción con isotiocianato de fluoresceína (Lens cullinaris y Hoeschst 33342 respectivamente bajo microscopía epifluorescente filtro FITC: 490nn y emisión 520nm. No hubo diferencias estadísticas entre los grupos control y tratamiento en la maduración in vitro, citoplasmática y nuclear de ovocitos de perras por el test de Kruskal – Wallis, p< 0,05. Palabras-clave: maduración, ovocito, perras, IGF-I pt Objetivou-se avaliar a adição do fator de crescimento recombinante IGF–I na concentração de 100?g/mL em 500µL de meio TCM 199 na maturação in vitro de oócitos caninos. Avaliou-se, com isso, o grau de maturação citoplasmática e nuclear por meio de coloração com isotiocianato de fluoresceína (Lens cullinaris e Hoeschst 33342, respectivamente, em microscópio epifluorescente filtro FITC: excitação 490nm e emissão 520nm. Como resultado, verificou-se que não houve diferenças estatísticas entre os grupos controle e tratamento na maturação in vitro, citoplasmática e nuclear, de oócitos caninos pelo

  19. Improvement of real pine (Pinus montezumae Lamb). Part I; Mejoramiento del pino real. Parte I

    Energy Technology Data Exchange (ETDEWEB)

    Gonzalez J, J.; Jimenez C, M.; Garcia T, M.A. [Instituto Nacional de Investigaciones Nucleares, Laboratorio de Genetica Vegetal, A.P. 18-1027, 11801 Mexico D.F. (Mexico)

    2000-07-01

    The real pine is an endemic specie of Mexico which is actually in danger of extinction. So the gamma radiation can accelerate the development of some vegetal species, then it is proceeded to find the radiation dose which allows to break the disease condition to diminish the lethargy time of those seeds with a dose range of 1 to 300 Krad of gamma radiation. It is required to realize more tests and observing in a greater period the hipocotile emission to determine the dose which allow to break the condition permanently. (Author)

  20. Assessment of the Effectiveness of Ectomycorrhizal Inocula to Promote Growth and Root Ectomycorrhizal Colonization in Pinus patula Seedlings Using the Most Probable Number Technique

    Directory of Open Access Journals (Sweden)

    Manuel Restrepo-Llano

    2014-01-01

    Full Text Available The aim of this study was to evaluate the response of Pinus patula seedlings to two inocula types: soil from a Pinus plantation (ES and an in vitro produced inoculum (EM. The most probable number method (MPN was used to quantify ectomycorrhizal propagule density (EPD in both inocula in a 7-order dilution series ranging from 100 (undiluted inoculum to 10−6 (the most diluted inoculum. The MPN method allowed establishing differences in the number of infective ectomycorrhizal propagules’ density (EPD (ES=34 per g; EM=156 per g. The results suggest that the EPD of an inoculum may be a key factor that influences the successfulness of the inoculation. The low EPD of the ES inoculum suggests that soil extracted from forest plantations had very low effectiveness for promoting root colonization and plant growth. In contrast, the high EPD found in the formulated inoculum (EM reinforced the idea that it is better to use proven high quality inocula for forest nurseries than using soil from a forestry plantation.

  1. Understory plant diversity assessment of Szemao pine (Pinus kesiya var. langbianensis plantations in Yunnan, China

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    Qiu, J. X.

    2012-12-01

    Full Text Available Sustainability is a key objective for managers of both natural forests and plantations, and biodiversity assessments are important tools to improve conservation of endangered species. Szemao pine (Pinus kesiya var. langbianensis is a native Chinese tree species used in plantations. This study evaluated differences in understory diversity among Szemao pine plantations (SP and other local current vegetation types: secondary evergreen forests (SE and abandoned farmlands (AF in Yunnan Province. Sampling was performed at three elevation ranges, where species richness, species cover, and environmental variables in the herb and shrub layers were measured. We found that indexes for average richness and Shannon–Wiener diversity were higher in SE than in SP, which were in turn higher than in AF, while the index for evenness was higher in SP. These indexes increased with elevation in SP and AF, but were higher at low and medium elevations in SE. Inclusion of environmental factors highlighted elevation differences, with water content (at herb layer and soil type (at shrub layer being the most significant variables. In conclusion, plantations of Szemao pine negatively affect understory diversity in Yunnan, and furthermore, only a few rare or threatened species could be found in the plantations. Nature reserves and transplanting could protect threatened species if established before plantations.La sostenibilidad es un objetivo clave para la gestión tanto de bosques naturales como de plantaciones, mientras que los estudios sobre biodiversidad constituyen herramientas muy útiles para mejorar la conservación de especies amenazadas. El pino Szemao (Pinus kesiya var. langbianensis es un árbol nativo de China que se usa en plantaciones. Este estudio evalúa la diversidad del sotobosque en plantaciones de pino Szemao (SP y otros tipos de vegetación local, como bosques secundarios perennifolios (SE y tierras de cultivo abandonadas (AF, en la provincia de

  2. Changes in soil quality after converting Pinus to Eucalyptus plantations in southern China

    Science.gov (United States)

    Zhang, K.; Zheng, H.; Chen, F. L.; Ouyang, Z. Y.; Wang, Y.; Wu, Y. F.; Lan, J.; Fu, M.; Xiang, X. W.

    2015-02-01

    Vegetation plays a key role in maintaining soil quality, but long-term changes in soil quality due to plant species change and successive planting are rarely reported. Using the space-for-time substitution method, adjacent plantations of Pinus and first, second, third and fourth generations of Eucalyptus in Guangxi, China were used to study changes in soil quality caused by converting Pinus to Eucalyptus and successive Eucalyptus planting. Soil chemical and biological properties were measured and a soil quality index was calculated using principal component analysis. Soil organic carbon, total nitrogen, alkaline hydrolytic nitrogen, microbial biomass carbon, microbial biomass nitrogen, cellobiosidase, phenol oxidase, peroxidase and acid phosphatase activities were significantly lower in the first and second generations of Eucalyptus plantations compared with Pinus plantation, but they were significantly higher in the third and fourth generations than in the first and second generations and significantly lower than in Pinus plantation. Soil total and available potassium were significantly lower in Eucalyptus plantations (1.8-2.5 g kg-1 and 26-66 mg kg-1) compared to the Pinus plantation (14.3 g kg-1 and 92 mg kg-1), but total phosphorus was significantly higher in Eucalyptus plantations (0.9-1.1 g kg-1) compared to the Pinus plantation (0.4 g kg-1). As an integrated indicator, soil quality index was highest in the Pinus plantation (0.92) and lowest in the first and second generations of Eucalyptus plantations (0.24 and 0.13). Soil quality index in the third and fourth generations (0.36 and 0.38) was between that in Pinus plantation and in first and second generations of Eucalyptus plantations. Changing tree species, reclamation and fertilization may have contributed to the change observed in soil quality during conversion of Pinus to Eucalyptus and successive Eucalyptus planting. Litter retention, keeping understorey coverage, and reducing soil disturbance during

  3. Laboratory and Simulated Field Bioassays to Evaluate Larvicidal Activity of Pinus densiflora Hydrodistillate, Its Constituents and Structurally Related Compounds against Aedes albopictus, Aedes aegypti and Culex pipiens pallens in Relation to Their Inhibitory Effects on Acetylcholinesterase Activity.

    Science.gov (United States)

    Lee, Dong Chan; Ahn, Young-Joon

    2013-05-30

    The toxicity of Pinus densiflora (red pine) hydrodistillate, its 19 constituents and 28 structurally related compounds against early third-instar larvae of Aedes albopictus (Ae. albopictus), Aedes aegypti (Ae. aegypti) and Culex pipiens palles (Cx. p. pallens) was examined using direct-contact bioassays. The efficacy of active compounds was further evaluated in semi-field bioassays using field-collected larval Cx. p. pallens. Results were compared with those of two synthetic larvicides, temephos and fenthion. In laboratory bioassays, Pinus densiflora hydrodistillate was found to have 24 h LC50 values of 20.33, 21.01 and 22.36 mg/L against larval Ae. albopictus, Ae. aegypti and Cx. p. pallens respectively. Among the identified compounds, thymol, δ-3-carene and (+)-limonene exhibited the highest toxicity against all three mosquito species. These active compounds were found to be nearly equally effective in field trials as well. In vitro bioassays were conducted to examine the acetylcholinesterase (AChE) inhibitory activity of 10 selected compounds. Results showed that there is a noticeable correlation between larvicidal activity and AChE inhibitory activity. In light of global efforts to find alternatives for currently used insecticides against disease vector mosquitoes, Pinus densiflora hydrodistillate and its constituents merit further research as potential mosquito larvicides.

  4. Laboratory and Simulated Field Bioassays to Evaluate Larvicidal Activity of Pinus densiflora Hydrodistillate, Its Constituents and Structurally Related Compounds against Aedes albopictus, Aedes aegypti and Culex pipiens pallens in Relation to Their Inhibitory Effects on Acetylcholinesterase Activity

    Directory of Open Access Journals (Sweden)

    Young-Joon Ahn

    2013-05-01

    Full Text Available The toxicity of Pinus densiflora (red pine hydrodistillate, its 19 constituents and 28 structurally related compounds against early third-instar larvae of Aedes albopictus (Ae. albopictus, Aedes aegypti (Ae. aegypti and Culex pipiens palles (Cx. p. pallens was examined using direct-contact bioassays. The efficacy of active compounds was further evaluated in semi-field bioassays using field-collected larval Cx. p. pallens. Results were compared with those of two synthetic larvicides, temephos and fenthion. In laboratory bioassays, Pinus densiflora hydrodistillate was found to have 24 h LC50 values of 20.33, 21.01 and 22.36 mg/L against larval Ae. albopictus, Ae. aegypti and Cx. p. pallens respectively. Among the identified compounds, thymol, δ-3-carene and (+-limonene exhibited the highest toxicity against all three mosquito species. These active compounds were found to be nearly equally effective in field trials as well. In vitro bioassays were conducted to examine the acetylcholinesterase (AChE inhibitory activity of 10 selected compounds. Results showed that there is a noticeable correlation between larvicidal activity and AChE inhibitory activity. In light of global efforts to find alternatives for currently used insecticides against disease vector mosquitoes, Pinus densiflora hydrodistillate and its constituents merit further research as potential mosquito larvicides.

  5. Paleoclimatic implications of glacial and postglacial refugia for Pinus pumila in western Beringia

    Energy Technology Data Exchange (ETDEWEB)

    Anderson, P M; Lozhkin, A V; Solomatkina, T B; Brown, T A

    2010-02-05

    Palynological results from Julietta Lake currently provide the most direct evidence to support the existence of a glacial refugium for Pinus pumila in mountains of southwestern Beringia. Both percentages and accumulation rates indicate the evergreen shrub survived until at least {approx}19,000 14C yr B.P. in the Upper Kolyma region. Percentage data suggest numbers dwindled into the late glaciation, whereas pollen accumulation rates point towards a more rapid demise shortly after {approx}19,000 14C yr B.P. Pinus pumila did not re-establish in any great numbers until {approx}8100 14C yr B.P., despite the local presence {approx}9800 14C yr B.P. of Larix dahurica, which shares similar summer temperature requirements. The postglacial thermal maximum (in Beringia {approx}11,000-9000 14C yr B.P.) provided Pinus pumila shrubs with equally harsh albeit different conditions for survival than those present during the LGM. Regional records indicate that in this time of maximum warmth Pinus pumila likely sheltered in a second, lower-elevation refugium. Paleoclimatic models and modern ecology suggest that shifts in the nature of seasonal transitions and not only seasonal extremes have played important roles in the history of Pinus pumila over the last {approx}21,000 14C yr B.P.

  6. ANTAGONISMO IN VITRO DE Trichoderma harzianum Rifai SOBRE Fusarium oxysporum Schlecht f. sp passiflorae EN MARACUYÁ (Passiflora edulis Sims var. Flavicarpa DEL MUNICIPIO ZONA BANANERA COLOMBIANA ANTAGONISM IN VITRO OF Trichoderma harzianum Rifai AGAINST Fusarium oxysporum Schlecht f. sp passiflorae IN PASSION FRUIT (Passiflora edulis Sims var. Flavicarpa FROM COLOMBIAN BANANERA ZONE MUNICIPALITY

    Directory of Open Access Journals (Sweden)

    Reinel José Fernández Barbosa

    2009-06-01

    Full Text Available Fusarium oxysporum Schlecht f. sp passiflorae causa la marchitez del maracuyá (Passiflora edulis Sims var. Flavicarpa, afectando su rendimiento. En la búsqueda de alternativas para su control se realizó la presente investigación con el objetivo de determinar la capacidad antagónica de 6 aislamientos de Trichoderma harzianum sobre dicho patógeno. Se evaluaron 3 aislamientos comerciales (TCC-001, TCC-005 y TCC-006 y 3 aislamientos nativos de suelo cultivado con palma de aceite en el Centro de Investigación Caribia de Corpoica (TCN-009, TCN-010, TCN-014. Se hizo la prueba in vitro empleando la técnica de cultivo dual en platos Petri con Agar Sabouraud. Se evaluó competencia por nutrientes y espacio, micoparasitismo y porcentaje de inhibición del crecimiento radial (PICR, por 10 días a 28 ºC. Se estableció un diseño completamente aleatorio, con 13 tratamientos y 3 repeticiones. Todos los aislamientos de T. harzianum superaron en crecimiento a F. oxysporum con radios de de 7,42 cm en cultivo dual. Mientras que el patógeno mostró un radio de 1,99 cm. TCN-009 y TCC-006 expresaron los mejores radios al crecer 4 veces mas rápido que F. oxysporum y reducir 3 veces menos el RCP con respecto al testigo, sin diferencias significativas entre estos tratamientos (P=0,0001; además, produjeron el mayor PICR a los 10 días con valores de 64,61 y 65,91%, respectivamente. No hubo diferencias significativas al comparar los aislamientos comerciales y nativos; sin embargo, por la naturaleza autóctona TCN-009, resulta ser a nivel in vitro, el aislamiento mas promisorio en el biocontrol de F. oxysporum por hallarse en condiciones agroclimáticas similares en la Zona Bananera Colombiana.Fusarium oxysporum Schlecht f. sp passiflorae cause withering of the passion fruit (Passiflora edulis Sims var. Flavicarpa, affecting their performance. In the search of alternatives for its control was carried out the present investigation with the objective of

  7. Efecto del nivel de inclusión de soya en la digestibilidad in vitro de la harina de piscidium de Moringa oleifera Effect of the soybean inclusion rate on the in vitro digestibility of the meal from Moringa oleifera pods

    Directory of Open Access Journals (Sweden)

    I. L Montejo

    2012-06-01

    Full Text Available El trabajo tuvo como objetivo evaluar el efecto de la inclusión de harina de soya, en forma de reactivo para simular el efecto de un concentrado proteínico, en la digestibilidad in vitro de la harina de piscidium (vainas de moringa. Se evaluaron nueve tratamientos de piscidium de moringa:soya (100:0; 90:10; 80:20; 70:30; 60:40; 50:50; 40:60; 20:80 y 0:100, con un diseño completamente aleatorizado y tres réplicas por cada tratamiento. Se observó un incremento en la producción de gas con el aumento del porcentaje de inclusión de soya; la digestibilidad verdadera tuvo un comportamiento similar. En cambio, la producción de biomasa microbiana alcanzó su mayor valor con la inclusión de la soya al 10%. Se concluye que la adición de la soya mejora la digestibilidad de las vainas de moringa, y que la eficiencia óptima de aprovechamiento de esta dieta se alcanza con la inclusión del 10% de soya.The objective of this work was to evaluate the effect of the inclusion of soybean meal, in this case in a way of a lab reactive, simulating a conventional protein concentrate, on the in vitro digestibility of the meal from Moringa oleifera pods. Nine treatments of M. oleifera pods:soybean were evaluated (100:0, 90:10, 80:20, 70:30, 60:40, 50:50, 40:60, 20:80 and 0:100, with a completely randomized design and three replications per treatment. An increase was observed in gas production with the increase of the soybean inclusion percentage; the true digestibility had a similar performance. On the contrary, the production of microbial biomass reached its higher value with the inclusion of 10% soybean. The addition of soybean is concluded to improve the digestibility of the M. oleifera pods, and the optimum utilization efficiency of this diet is reached with the inclusion of 10% soybean.

  8. Chemical properties of tannic extracts from bark of Pinus oocarpa and their use as adhesive

    Directory of Open Access Journals (Sweden)

    Michel Cardoso Vieira

    2014-03-01

    Full Text Available This work evaluated the properties of aqueous extracts obtained from the bark of Pinus oocarpa under addition of sodium sulfite and sodium bisulfite and the possibility of employment of tannins from the bark as an adhesive for bonding wood. After evaluation of the chemical properties of tannic extracts it was decided to employ the extraction with distilled water under addition of / 5% sodium sulfite to prepare for the tannin-formaldehyde adhesive. Adhesive phenol formaldehyde and urea-formaldehyde were modified with 10% tannin Pinus oocarpa and the effect of this addition on the quality of the adhesive was evaluated. The addition from the bark of Pinus oocarpa showed that it is possible to use pure tannin as an adhesive because of its good gluing characteristics. The addition of tannic extract to synthetic adhesives contributed to increase viscosity values. Thus the substitution of synthetic adhesives by tannins is possible only up to 10%.

  9. Pest risk assessment of the importation into the United States of unprocessed Pinus and Abies logs from Mexico

    Science.gov (United States)

    B. M. Tkacz; H. H. Burdsall; G. A. DeNitto; A. Eglitis; J. B. Hanson; J. T. Kliejunas; W. E. Wallner; J. G. O`Brien; E. L. Smith

    1998-01-01

    The unmitigated pest risk potential for the importation of Pinus and Abies logs from all states of Mexico into the United States was assessed by estimating the probability and consequences of establishment of representative insects and pathogens of concern. Twenty-two individual pest risk assessments were prepared for Pinus logs, twelve dealing with insects and ten...

  10. Optimización de un protocolo de extracción de ADN genómico para Pinus tecunumanii

    Directory of Open Access Journals (Sweden)

    David A. Cerda-Granados

    2013-05-01

    Full Text Available Se optimizó un protocolo para la extracción de ADN genómico de Pinus tecunumanii basado en el método de extracción de Doyle y Doyle (1990. Se probaron los megagametofitos de las semillas de árboles deP. tecunumanii muestreados en cinco poblaciones naturales de Nicaragua. El método consta de maceración del tejido en tubos Eppendorf, una extracción con bromuro trimetil amonio de cetilo (CTABempleando altas concentraciones de sales, proteinasa K, extracciones sucesivas con cloroformo-alcohol isoamílico y un tratamiento con ARNasa A. El rendimiento fue aproximadamente 13μg de ADN por 58.7 mg de tejido inicial fresco. El ADNgenómico obtenido por este método fue apropiado para ser usado en reaccionesRAPD (ADN polimórfico amplificado al azar

  11. Efecto in vitro de aceites esenciales de tres especies de Lippia sobre Moniliophthora roreri (Cif. y Par. Evans et al., agente causante de la moniliasis del cacao (Theobroma cacao L.

    Directory of Open Access Journals (Sweden)

    Lozada Betty Stefany

    2012-11-01

    Full Text Available

    Se evaluó el efecto antifúngico in vitro de cinco aceites esenciales (AEs (AE1, AE2, AE3, AE4 y AE5 extraídos de Lippia origanoides, L. citriodora y L. alba sobre aislados de monilia (Moniliophthora roreri obtenidos de frutos de cacao infectados provenientes de San Vicente de Chucurí, Santander, Colombia. Las plantas de Lippia fueron colectadas en cinco localidades colombianas. Los aislados de monilia (M1, M2, M3, M4 y M5 fueron caracterizados por su morfología, germinación y crecimiento en medios de cultivo. La actividad antifúngica de diferentes concentraciones de los AEs fue evaluada contra el aisla- do M2 y la cepa de M. roreri (ATCC 64239, para determinar su efecto sobre la germinación y la inhibi- ción del crecimiento micelial. Los AEs estudiados inhibieron 100% de la germinación y del crecimiento micelial cuando fueron utilizados en concentraciones de 800 - 1000 µg/ml. Concentraciones de 200 µg/ml también mostraron efecto sobre los aislamientos fúngicos, siendo los AEs obtenidos de L. ori- ganoides (AE2 y AE3 los más activos. Estos estaban compuestos principalmente por timol, p-cimeno,

    y-terpineno, acetato de timilo, carvacrol, 1-mirceno, trans-1ccariofileno. Diferencias significativas (P <

    0.05 sobre la susceptibilidad se observaron entre las dos cepas fúngicas estudiadas, siendo en gene- ral más susceptible el aislado M2 que la cepa ATCC. Los AEs de L. origanoides son candidatos para ser usados como posibles biofungicidas en el control de la moniliasis. Son necesarios estudios futuros orientados a determinar la actividad in vivo antifúngica de estos AEs y sus principales componentes.

  12. Factores que influyen en la producción de planta de Pinus cooperi blanco en vivero

    Directory of Open Access Journals (Sweden)

    J. Á. Prieto Ruíz

    2004-01-01

    Full Text Available Con el objetivo de evaluar el efecto de tres factores en el crecimiento y supervivencia de Pinus cooperi Blanco en vivero, se realizó un experimento en el Campo Experimental Valle del Guadiana-INIFAP, Durango, Dgo., México. Los tratamientos se distribuyeron en un diseño experimental de bloques al azar con un arreglo en parcelas subdivididas y consistieron en: a dos tamaños de envase: 80 y 170 cm3 de volumen, b tres rutinas de fertilización, con diferentes proporciones de nitrógeno (N, fósforo (P y potasio (K, con dosis que variaron en las tres fases de crecimiento de las plantas y c tres frecuencias de riego aplicadas cada 48, 96 y 168 horas durante la fase de preacondicionamiento. A los ocho meses de edad se encontró que la planta producida en el envase de 170 cm3 fue estadísticamente superior en las variables altura, diámetro y producción de fitomasa. En cambio, las rutinas de fertilización no tuvieron efectos significativos en las variables estudiadas. El riego cada 48 horas en la fase de preacondicionamiento también influyó significativamente en las variables medidas. La supervivencia no mostró diferencias significativas a las fuentes de variación estudiadas.

  13. PROYECTO DE ASERRADERO DE PINUS HALEPENSIS EN VIVER (CASTELLÓN)

    OpenAIRE

    MORILLO ROSELLÓ, ALEJANDRO JAVIER

    2015-01-01

    [ES] EL PRESENTE TRABAJO TIENE COMO OBJETIVO LA ELABORACIÓN DE UN PROYECTO DE INGENIERÍA PARA LA IMPLANTACIÓN DE UNA INDUSTRIA DE ASERRADO DE CONÍFERAS ESPECIALIZADA EN PINUS HALEPENSIS EN EL TÉRMINO MUNICIPAL DE VIVER (CASTELLÓN). CONSTA DE LOS SIGUIENTES DOCUMENTOS: MEMORIA Y ANEJOS, PLANOS, PLIEGO DE CONDICIONES, PRESUPUESTO Y ESTUDIO DE SEGURIDAD Y SALUD. Morillo Roselló, AJ. (2015). PROYECTO DE ASERRADERO DE PINUS HALEPENSIS EN VIVER (CASTELLÓN). http://hdl.handle.net/10251/55988. ...

  14. Antibacterial potential of essential oils of the needles of Pinus halepensis against Staphylococcus aureus and Escherichia coli

    Directory of Open Access Journals (Sweden)

    Ghalem Bachir Raho

    2014-08-01

    Full Text Available Objective: To examine the in vitro antimicrobial activities of essential oil of the needles of Pinus halepensis (P. halepensis. Methods: The antibacterial activity of essential oil of the needles of P. halepensis was determined using the agar well diffusion technique and disc diffusion method against Staphylococcus aureus ATCC 25923, and Escherichia coli ATCC 25922. Results: The diameter of zones of inhibition exhibited by the essential oil was between 6 and 17 mm. The essential oils was compared favorably with gentamycin used as a standard control. The minimum inhibitory concentration determined by the agar well diffusion method was 0.52 mg/ mL for Staphylococcus aureus and 2.15 mg/mL for Escherichia coli. The minimum bactericidal concentration of the oils against the two microorganisms was 4.17 mg/mL. Conclusions: The results obtained from this study reveal that P. halepensis essential oils possess antibacterial activities and can be used as antimicrobial agents in the search for new drugs.

  15. Ash properties of Pinus halepensis needles treated with diammonium phosphate

    Energy Technology Data Exchange (ETDEWEB)

    Liodakis, S.; Katsigiannis, G.; Lymperopoulou, T. [Laboratory of Inorganic and Analytical Chemistry, Department of Chemical Engineering, National Technical University of Athens (NTUA), 9 Iroon Polytechniou Street, Athens 157 73 (Greece)

    2007-02-01

    The ash properties of Pinus halepensis (Aleppo pine) needles before and after treatment with diammonium phosphate (NH{sub 4}){sub 2}HPO{sub 4} (DAP) have been investigated, using thermogravimetric analysis (TG), differential thermal analysis (DTA), titrimetry, inductively coupled plasma-emission spectrometry (ICP-ES), X-ray diffraction (XRD) and scanning electron microscopy (SEM). DAP is extensively used as active component in wildland fire retardants. The following crystalline compounds have been identified in ashes prepared at 600 C before treatment with DAP: KCl, Ca(OH){sub 2}, MgO, (CaMg)CO{sub 3}, K{sub 2}CO{sub 3}.CaCO{sub 3}, K{sub 2}CO{sub 3}, K{sub 2}SO{sub 4}, CaO and CaCO{sub 3}, whereas CaO, MgO, K{sub 2}SO{sub 4}, K{sub 2}CO{sub 3}, CaCO{sub 3}, KCl and CaO, MgO, K{sub 2}SO{sub 4} and K{sub 2}CO{sub 3} at 800 and 1000 C, respectively. The presence of DAP alters the composition of ashes converting, almost completely at high temperatures, the metallic oxides into phosphate salts. Thus, decreasing their alkalinity. The micrographs obtained by SEM indicate that pine needles ashes contain large porous particles of carbon compounds and several inorganic particles of irregular shape <1.0 mm, whereas after treating the needles with DAP an amorphous rigid structure was formed. To facilitate our investigation model mixtures of CaCO{sub 3} + DAP, MgCO{sub 3} + DAP, K{sub 2}CO{sub 3} + DAP were heat treated under the same conditions used for preparing the ashes. The chemical transformations taken place during heating were studied by analysing the reaction products using thermal analysis and XRD. The physical, mineralogical and chemical forest ash properties determined could be used to evaluate the environmental risk of the use of fire retardants on soils, plants and aquatic systems as well as to investigate the mechanism of combustion of forest fuels in the presence of DAP. (author)

  16. Micropropagation of an endangered species Pinus armandii var. Amamiana

    Directory of Open Access Journals (Sweden)

    Katsuaki Ishii

    2013-12-01

    Full Text Available For micropropagation via organ culture, mature embryos were excised from the seeds of Pinus armandii. Franch. var. amamiana (Koidz. Hatusima, an endangered species only inhabiting the south west islands of Japan. Adventitious buds were induced on the surface of the embryo on 1/2 DCR medium containing BAP, and they grew shoots after subculturing to medium containing activated charcoal or a low concentration of thidiazuron. From the elongated shoots, root primordia and roots were induced in medium containing IBA as an auxine. We found that a low concentration of zeatin or BAP added to the medium was beneficial for plant regeneration of mature embryos of this species. For micropropagation via somatic embryogenesis, embryogenic cell suspensions were induced from a mature and immature seed of P. armandii var. amamiana on MS liquid medium supplemented with 1 ľM 2, 4-D and 3 ľM BAP. The suspensions were incubated in the dark at 250. Induced suspension cells were transferred to ammonium free MS liquid medium supplemented with 1 ľM 2, 4-D, 3 ľM BAP and 30m M L-glutamine and subcultured every 2 weeks. In the other set of the experiment, the induction rate of somatic embryogenesis was high with ammonium free half strength MS medium. In order to develop somatic embryos, the suspension cells were transferred to ammonium free MS medium supplemented with 10 ľM ABA, 0.2% activated charcoal, 10% PEG (MW6000, 30m M L-glutamine and 6% maltose. The cultures were incubated under a 16h light/8h dark photoperiod. After 1-2 months of culture, differentiation of embryos progressed and cotyledonary embryos were obtained. These embryos were transferred on ammonium free MS solid medium under 16 h photoperiod. After 2-3 weeks plantlets with roots and green cotyledons were obtained. Plantlets were transplanted to vermiculite containing modified MS liquid medium in 200 ml culture flasks, then out planted after habituation procedure.

  17. Seed source variation and conservation of Pinus wallichiana in India

    Directory of Open Access Journals (Sweden)

    M. Thapliyal

    2008-09-01

    Full Text Available Pinus wallichiana A.B. Jacks is an important component of the middleand high altitude Himalayan forests having large natural distribution ranging between 260 to 360 N latitude and 690 to 750 E longitudes. It is commonly known as Himalayan blue pine or blue pine, being indigenous to Himalayan Mountain regions and because of its bluish or grayish-green leaves. It is a five needle pine which gained world-wide attention for its resistance to blister rust among white pines. This species has been crossed successfully with other white pines and vigorous hybrids have been obtained. Considerable variation in morphological and anatomical characteristics of needles, cones and seeds in natural stands exists across the natural distribution of the species, especially in mesic and xeric habitats. These variationssuggested the differentiation of this species in ecotypes or varieties as reported by various authors. However, the level of genetic diversity was found to be relatively high and the degree of genetic differentiation was low compared to other pines. Thewide range of climatic conditions in the natural distribution of this pine is expected to result in high genetic variation within different populations of the species. The study aims to determine the nature and extent of variation present in the populations of the species in respect to cone and seed characteristics across its natural distribution. Seed of 17 seed sources from the states of Uttaranchal and Himachal Pradesh was collected and analyzed for cone characters (fresh weight of cones, cone length, cone width, specific gravity and seed characters (seed weight, moisture content, germination percent, cotyledon number. Significant variations have beenobserved in these traits among different seed sources of the species. The cone weight varied from 44.4 to114g and the higher cone weight was recorded at higher altitudes.The germination percent varied from 40 to 85 whereas cotyledon number varied from 7

  18. Xylem hydraulic efficiency versus vulnerability in seedlings of four contrasting Mediterranean tree species (Cedrus atlantica, Cupressus sempervirens, Pinus halepensis and Pinus nigra)

    OpenAIRE

    Froux, Fabienne; Huc, Roland; Ducrey, Michel; Dreyer, Erwin

    2002-01-01

    International audience; We studied the xylem hydraulic traits and anatomy of four diverse Mediterranean conifers to determine how these species protect themselves against catastrophic xylem failure. Cedrus atlantica, Cupressus sempervirens, Pinus nigra and P. halepensis seedlings were grown for two years in pots in a greenhouse under well-watered conditions. Measurements were conducted in April and September. The vulnerability to cavitation was lower in April in the two pines and cedar wherea...

  19. Study on pinewoods of pinus halepensis of the Gargano zone; Ricerche sulle pinete di Pino d`Aleppo (Pinus halepensis del Gargano Forestale)

    Energy Technology Data Exchange (ETDEWEB)

    La Marca, O. [Florence Univ. (Italy). Ist. di Assestamento e Tecnologia Forestale; La Marca, D.

    1996-02-01

    The present work, carried out in a permanent test area, aims to establish the influence of thinning on the evolution of populations, growth and on the risk of forest fires. Surveys of the times required for inter cropping interventions have also been carried out. Thinning in the test areas had only a slight effect of growth. Natural mortality was higher in the un thinned areas. Some twenty years after the first interventions, a further inter cropping intervention is needed not only to improve the conditions of the vegetation, but also to reduce the risk of forest fires.

  20. CRECIMIENTO Y CONTENIDO NUTRIMENTAL DE Pinus greggii Engelm. INOCULADO CON EL HONGO COMESTIBLE ECTOMICORRÍZICO Hebeloma mesophaeum (Pers. Quél.

    Directory of Open Access Journals (Sweden)

    Magdalena Martínez-Reyes

    2012-01-01

    Full Text Available Se evaluó el crecimiento y contenido de macro y micronutrimentos de Pinus greggii Engelm., cuando éste fue inoculado con el hongo comestible ectomicorrízico Hebeloma mesophaeum (Pers. Quél. Se tuvieron dos tratamientos: plantas inoculadas con H. mesophaeum y plantas sin inocular. Los resultados mostraron que tanto el peso seco de la parte aérea y de la raíz, altura y diámetro del tallo así como el contenido total de N, P, K, Ca y Mg, fueron mayores en las plantas inoculadas con respecto a las plantas no inoculadas. Los contenidos de P y Mg fueron, respectivamente, 6.7 y 6.9 veces mayor en la parte aérea de las plantas inoculadas que las no inoculadas. Adicionalmente, existió una eficiencia alta de H. mesophaeum para la traslocación de P, K y Mg a la parte aérea de las plantas. El porcentaje de micorrización fue alto (79.5 %, del cual, más de la mitad se observó en la parte media del cepellón. La inoculación con H. mesophaeum tiene entonces un gran potencial de uso en la producción de plantas de P. greggii en invernadero.

  1. Cambios en la comunidad de Pinus culminicola Andersen & Beaman en el Cerro Potosí, N.L. México

    Directory of Open Access Journals (Sweden)

    Rubén Sánchez Silva

    1987-01-01

    Full Text Available Se realizó un estudio en la cima del Cerro Potosí, N.L., acerca de Pinus culminicola Andresen & Beaman, especie endémica del norte de la Sierra Madre Oriental, que hasta 1960 cubría 106 ha, mientras que en 1970 esta superficie había disminuido a 70 ha debido a que el resto fue destruido por el fuego. Mediante el análisis de fotografías aéreas y trabajo de campo se determino la distribución y la estructura florística de las asociaciones vegetales del área, caracterizándose sus condiciones ambientales. Aunque se detecto que existe cierta regeneración de P. culminicola, ésta es insuficiente para contrarrestar las alte­raciones causadas por la actual y creciente presión antrópica; de lo anterior se desprende la necesidad de decretar dicha zona como área protegida, de modo que se garantice su efectiva conservación.

  2. Simulatd Nitrogen Cycling Response to Elevated CO2 in Pinus taeda and Mixed Dediduous Forests

    Science.gov (United States)

    D.W. Johnson

    1999-01-01

    Interactions between elevated CO2 and N cycling were explored with a nutrient cycling model (NuCM, Johnson et al. 1993, 1995) for a Pinus tuedu L. site at Duke University North Carolina, and a mixed deciduous site at Walker Branch, Tennessee. The simulations tested whether N limitation would prevent growth increases in response to elevated CO...

  3. Implications of foliar terpene content and hydration on leaf flammability of Quercus ilex and Pinus halepensis.

    Science.gov (United States)

    Alessio, G A; Peñuelas, J; De Lillis, M; Llusià, J

    2008-01-01

    We investigated the implications of foliar hydration and terpene content on leaf flammability in two widely distributed forest species of the Mediterranean basin, Quercus ilex, which does not store terpenes, and Pinus halepensis, a terpene-storing species. The experiments were carried out in plants grown under different water regimes that generated a wide range of foliar hydration and terpene contents. We monitored the temperatures and time elapsed to reach the smoke, pyrolysis and flame phases. Smoke appeared much earlier (37 versus 101 s) and at lower temperatures (96 versus 139 degrees C) in Quercus ilex than in Pinus halepensis. Quercus ilex reached pyrolysis earlier than Pinus halepensis (278 versus 338 s) but at the same temperature (365-371 degrees C). There were no significant differences in time elapsed nor in temperature for flammability (386-422 s; 505-487 degrees C in both species). Quercus ilex had lower water hydration than Pinus halepensis (41 versus 100%) and the leaf content of terpenes in Quercus was three orders of magnitude lower. The results of this study show no differences in the flame phase between the two species and the absence of a significant relationship between temperature and elapsed time of the different flammability phases in relation to monoterpene content; thus indicating that the role of monoterpenes in flammability phases is smaller than that of the water content. This, however, does not exclude the effects of terpene content on plant combustibility and fire propagation once fires start.

  4. The effect of mid-rotation fertilization on the wood properties of loblolly pine (Pinus taeda)

    Science.gov (United States)

    Finto Antony; Lewis Jordan; Laurence R. Schimleck; Richard F. Daniels; Alexander Clark III

    2009-01-01

    Mid-rotation fertilization is a common practice in the management of loblolly pine (Pinus taeda L.) plantations, typically providing large improvements in growth. However, concerns exist about the quality of wood produced following fertilization. The objective of this study was to develop an understanding of wood property changes following fertilization. Wood...

  5. Climate-related genetic variation in a threatened tree species, Pinus albicaulis

    Science.gov (United States)

    Marcus V. Warwell; Ruth G. Shaw

    2017-01-01

    PREMISE OF THE STUDY: With ongoing climate change, understanding of intraspecific adaptive variation is critical for conservation and restoration of plant species. Such information is especially scarce for threatened and endangered tree species, such as Pinus albicaulis Engelm. Therefore, our principal aims were to assess adaptive variation and characterize its...

  6. Modeling contemporary climate profiles of whitebark pine (Pinus albicaulis) and predicting responses to global warming

    Science.gov (United States)

    Marcus V. Warwell; Gerald E. Rehfeldt; Nicholas L. Crookston

    2006-01-01

    The Random Forests multiple regression tree was used to develop an empirically-based bioclimate model for the distribution of Pinus albicaulis (whitebark pine) in western North America, latitudes 31° to 51° N and longitudes 102° to 125° W. Independent variables included 35 simple expressions of temperature and precipitation and their interactions....

  7. First discovery of fossil winged seeds of Pinus L. (family Pinaceae ...

    Indian Academy of Sciences (India)

    Mahasin Ali Khan

    2017-07-04

    Jul 4, 2017 ... documented from the Cenozoic sediments of eastern Asia (Japan and China), but none has been confirmed from the Indian ... This record suggests that Pinus was an important component of tropical-subtropical evergreen forest in the area ... stone and mudstone facies, with the finer sediments very often ...

  8. Regeneration Methods Affect Genetic Variation and Structure in Shortleaf Pine (Pinus Echinata Mill.)

    Science.gov (United States)

    Rajiv G. Raja; Charles G. Tauer; Robert F. Wittwer; Yinghua Huang

    1998-01-01

    The effects of regene ration methods on genetic diversity and structure in shortleaf pine (Pinus echinata Mill.) were examined by quantifying the changes in genetic composition of shortleaf pine stands following harvest by monitoring changes in allele number and frequency at heterozygous loci over time. The results were also compared to the genetic...

  9. Growth and physiological responses to varied environments among populations of Pinus ponderosa

    Science.gov (United States)

    Jianwei Zhang; Bert M. Cregg

    2005-01-01

    We investigated population responses in physiology, morphology, and growth of mature Pinus ponderosa trees to an environmental gradient across Nebraska, USA. Ten populations from western Nebraska and eastern Wyoming were grown in three 26-year-old provenance tests from the warmest and wettest site in the east (Plattsmouth) to the intermediate site in...

  10. Soil enzyme activities in Pinus tabuliformis (Carriere) plantations in northern China

    Science.gov (United States)

    Weiwei Wang; Deborah Page-Dumroese; Ruiheng Lv; Chen Xiao; Guolei Li; Yong Liu

    2016-01-01

    Changes in forest stand structure may alter the activity of invertase, urease, catalase and phenol oxidase after thinning Pinus tabuliformis (Carriére) plantations in Yanqing County of Beijing, China. We examined changes in these soil enzymes as influenced by time since thinning (24, 32, and 40 years since thinning) for 3 seasons (spring, summer and autumn)...

  11. Penafsiran Volume Pohon Pinus merkusii melalui Foto Udara (Studi Kasus BKPH Majenang, KPH Banyumas Barat

    Directory of Open Access Journals (Sweden)

    Sahid -

    2013-01-01

    Full Text Available Interpretation of  Stock Volume of Pinus merkusii  using Aerial Photographs (Case Study in BKPH Majenang, West Banyumas FMU The study aims to compile and analyse the growing stock volume of Pinus merkusii by using stand parameter measured through aerial photographs. The study was conducted by using black-white panchromatic aerial photographs produced in 2005 and the scale was 1 : 20,000. The study was conducted in Pinus merkusii taping plots in the Majenang BKPH, under the forest districts of west Banyumas, Perum Perhutani using tree height, crown diameter and number of trees per hectare as parameters. Data analysis was done to define the relationship among these parameters in regression equation. The steps of the study were defining measurement plots in aerial photographs, measurement of stand parameters in the aerial photographs and field observation. Data analysis was performed using micro computer. As a result, the regression equation for each location as follows: V = 72,415 – 0,231 N + 35,242 H – 24,454D – 0,923 (N x D, with the determination coefficient was 0,789 and the significance of 95%. Where: V = growing stock volume (m3, N = the number of trees per hectare, H = tree height (m and D = crown diameter (m. It was concluded that number of tree per hectare, tree height and crown diameter were good predictors for standing stock volume of Pinus merkusii.

  12. Vector Analysis Identify Loblolly Pine (Pinus taeda L.) Phosphorus Deficiency on a Beauregard Soil

    Science.gov (United States)

    A. Bekele; W.H. Hundall; A.E. Tiarks

    1999-01-01

    We studied the response of densely stocked one-year-old loblolly pine (Pinus taeda L.) to N and P fertilizers on a Beauregard silt loam (fine silty, siliceous, thermic, Plinthaquic Paleudults). A continuous function" experimental design with three replications was used. Each replication consisted of 12 m X 12 m plots, with three trees planted...

  13. Alkanes and terpenes in wood and leaves of Pinus jeffreyi and P. sabiniana

    Science.gov (United States)

    Robert P. Adams; Jessica W. Wright

    2012-01-01

    The wood oils of Pinus jeffreyi and P. sabiniana contain considerable amounts of heptane (76.6%, 92%), on a monoterpene basis. However, when entire wood extractables is considered, the amounts drop considerably (3.4%, 36.8%) with the major portion of the wood oils being diterpene acids. The leaf oil of P. jeffreyi...

  14. First discovery of fossil winged seeds of Pinus L.(family Pinaceae ...

    Indian Academy of Sciences (India)

    Here, we describe Pinus arunachalensis Khan and Bera, sp. nov. on the basis of seed remains from the middle to late Miocene Siwalik sediments of the Dafla Formation exposed around West Kameng district in Arunachal Pradesh, eastern Himalaya. Seeds are winged, broadly oblong to oval in outline, 1.3–1.5 cm long and ...

  15. Biomass distribution and productivity of Pinus edulis-Juniperus monosperma woodlands of north-central Arizona

    Science.gov (United States)

    Charles C. Grier; Katherine J. Elliott; Deborah G. McCullough

    1992-01-01

    Above-ground biomass distribution, leaf area, above-ground net primary productivity and foliage characteristics were determined for 90- and 350-year-old Pinus edulis-Juniperus monosperma ecosystems on the Colorado Plateau of northern Arizona. These ecosystems have low biomass, leaf area and primary productivity compared with forests in wetter...

  16. Biomass and nutrients of Pinus massoniana plantations in southern China: simulations for different management practices.

    Science.gov (United States)

    Huixia Yang; Silong Wang; Jianwei Zhang; Bing Fan; Weidong Zhang

    2011-01-01

    We measured the dynamics of both biomass and nutrient pools on 7-, 17-, 31- and 51-year-old Pinus massoniana plantations in southern China. Using a chronosequence approach, we found that biomass of each component increased with aging while its proportion decreased except stem-wood. Nutrient pools varied with biomass pools except for foliage. For all harvest intensities...

  17. Forest floor depth mediates understory vigor in xeric Pinus palustris ecosystems

    Science.gov (United States)

    J. Kevin Hiers; Joseph J. O' Brien; Rodney E. Will; Robert J. Mitchell

    2007-01-01

    Longleaf pine (Pinus palustris) woodlands and savannas are among the most frequently burned ecosystems in the world with fire return intervals of 1–10 years. This fire regime has maintained high levels of biodiversity in terms of both species richness and endemism. Land use changes have reduced the area of this ecosystem by .95%, and inadequate fire...

  18. Fungal endophytes in woody roots of Douglas-fir (Pseudotsuga menziesii) and ponderosa pine (Pinus ponderosa)

    Science.gov (United States)

    J. A. Hoff; Ned B. Klopfenstein; Geral I. McDonald; Jonalea R. Tonn; Mee-Sook Kim; Paul J. Zambino; Paul F. Hessburg; J. D. Rodgers; T. L. Peever; L. M. Carris

    2004-01-01

    The fungal community inhabiting large woody roots of healthy conifers has not been well documented. To provide more information about such communities, a survey was conducted using increment cores from the woody roots of symptomless Douglas-fir (Pseudotsuga menziesii) and ponderosa pine (Pinus ponderosa) growing in dry forests...

  19. Growth in relation to canopy light interception in a red pine (Pinus resinosa) thinning study

    Science.gov (United States)

    Beverly E. Law; Kurt H. Riitters; Lewis F. Ohmann

    1992-01-01

    Growth data from the most recent 5 years of a 40-year thinning study in an even-aged red pine (Pinus resinosa) forest in cutfoot sioux experimental forest, Minnesota, were used with intercepted photosynthetically active radiation (IPAR) data to determine the relationship between light interception and growth for a range ofstand densities. Stand basal...

  20. Effects of air pollution on morphological and anatomical characteristics of Pinus Eldarica Wood

    Science.gov (United States)

    Vahidreza Safdari; Moinuddin Ahmed; Margaret S. Devall; Vilma Bayramzadeh

    2012-01-01

    Air pollution, including automobile exhaust pollution, can affect anatomical and morphological characteristics of wood. In order to evaluate this subject, the Pinus eldarica trees of Chitgar Park in Tehran, which extends from a crowded highway in the south (polluted site) to the semi polluted midsection and to Alborz Mountain in the north (unpolluted...

  1. Results from four Pinus patula water planting trials in the summer ...

    African Journals Online (AJOL)

    Planting with water is used by some forestry companies in South Africa to reduce post-planting water stress. Four trials were implemented to test the response in survival of Pinus patula to water applied at planting. Two trials each were situated in the KwaZulu-Natal Midlands and Mpumalanga escarpment. The first trial at ...

  2. Polyamine levels during the development of zygotic and somatic embryos of Pinus radiata

    Science.gov (United States)

    Rakesh Minocha; Dale R. Smith; Cathie Reeves; Kevin D. Steele; Subhash C. Minocha

    1999-01-01

    Changes in the cellular content of three polyamines (putrescine, spermidine and spermine) were compared at different stages of development in zygotic and somatic embryos of Pinus radiata D. Don. During embryo development, both the zygotic and the somatic embryos showed a steady increase in spermidine content, with either a small decrease or no...

  3. DNA analysis for section identification of individual Pinus pollen grains from Belukha glacier, Altai Mountains, Russia

    Science.gov (United States)

    Nakazawa, Fumio; Uetake, Jun; Suyama, Yoshihisa; Kaneko, Ryo; Takeuchi, Nozomu; Fujita, Koji; Motoyama, Hideaki; Imura, Satoshi; Kanda, Hiroshi

    2013-03-01

    Pollen taxon in sediment samples can be identified by analyzing pollen morphology. Identification of related species based on pollen morphology is difficult and is limited primarily to genus or family. Because pollen grains of various ages are preserved at below 0 °C in glaciers and thus are more likely to remain intact or to suffer little DNA fragmentation, genetic information from such pollen grains should enable identification of plant taxa below the genus level. However, no published studies have attempted detailed identification using DNA sequences obtained from pollen found in glaciers. As a preliminary step, this study attempted to analyze the DNA of Pinus pollen grains extracted from surface snow collected from the Belukha glacier in the Altai Mountains of Russia in the summer of 2003. A 150-bp rpoB fragment from the chloroplast genome in each Pinus pollen grain was amplified by polymerase chain reaction, and DNA products were sequenced to identify them at the section level. A total of 105 pollen grains were used for the test, and sequences were obtained from eight grains. From the sequences obtained, the pollen grains were identified as belonging to the section Quinquefoliae. Trees of the extant species Pinus sibirica in the section Quinquefoliae are currently found surrounding the glacier. The consistency of results for this section suggests that the pollen in the glacier originated from the same Pinus trees as those found in the immediate surroundings.

  4. Water availability and genetic effects on wood properties of loblolly pine (Pinus taeda)

    Science.gov (United States)

    C. A. Gonzalez-Benecke; T. A. Martin; Alexander Clark; G. F. Peter

    2010-01-01

    We studied the effect of water availability on basal area growth and wood properties of 11-year-old loblolly pine (Pinus taeda L.) trees from contrasting Florida (FL) (a mix of half-sib families) and South Carolina coastal plain (SC) (a single, half-sib family) genetic material. Increasing soil water availability via irrigation increased average wholecore specific...

  5. Exome genotyping, linkage disequilibrium and population structure in loblolly pine (Pinus taeda L.)

    Science.gov (United States)

    Mengmeng Lu; Konstantin V. Krutovsky; C. Dana Nelson; Tomasz E. Koralewski; Thomas D. Byram; Carol A. Loopstra

    2016-01-01

    Background: Loblolly pine (Pinus taeda L.) is one of the most widely planted and commercially important foresttree species in the USA and worldwide, and is an object of intense genomic research. However, whole genomeresequencing in loblolly pine is hampered by its large size and complexity and a lack of a good...

  6. Seasonal ectomycorrhizal fungal biomass development on loblolly pine (Pinus taeda L.) seedlings

    Science.gov (United States)

    Shi-Jean S. Sung; L.M. White; D.H. Marx; W.J. Otrosina

    1995-01-01

    Ergosterol, a membrane sterol found in fungi but not in plants, was used to estimate live mycelia biomass in ectomycorrhizae. Loblolly pine (Pinus taeda L.) seeds were sown in April 1993 and grown with standard nursery culture ractices. Correlations between total seedling ergosterol and visual assessment of mycorrhizal colonization were high during...

  7. Studies on Ectomycorrhizae—XV. Mycorrhizae formed by Rhizopogon luteolus on Pinus silvestris

    NARCIS (Netherlands)

    Uhl, M.

    1988-01-01

    The mycorrhizae of Rhizopogon luteolus on Pinus silvestris are comprehensively described. They are characterized by a coralloid habit, ochre to yellow colour, highly differentiated rhizomorphs, a two-layered mantle and a differentiated autofluorescence in mantle, Hartig net and rhizomorphs. The

  8. Using flotation in ethanol to separate filled and empty seeds of Pinus ...

    African Journals Online (AJOL)

    In this study, the separation of filled and empty seeds of Crimean pine [Pinus nigra Arn. ssp. pallasiana (Lamb.) Holmboe] by flotation in ethanol and the effect of this treatment on seed germination were investigated. Flotation tests in 96% ethanol by the density method and then germination tests were made on the seeds ...

  9. Site and stand age effects on fertiliser responses in Pinus patula ...

    African Journals Online (AJOL)

    Results are discussed in relation to nutrient cycling, stand development, soil fertility and records of long-term productivity at Usutu. It is proposed that observed declines in second rotation ... Keywords: Fertiliser response, 2R decline, Pinus patula, Stand age, Soil nutrition. Southern African Forestry Journal No.199 2003: 27- ...

  10. Reproduction ecology of Pinus halepensis : a monoecious, wind-pollinated and partially serotinous Mediterranean pine tree

    NARCIS (Netherlands)

    Goubitz, Shirrinka

    2001-01-01

    Fire is an important factor in the evolution and ecology of Mediterranean plant species. The fire frequency has increased in the 20st century. Pines are the most important tree species in the area. Pinus halepensis is the only natural pine in parts of the east Mediterranean basin, such as Israel and

  11. Fusarium spp. and Pinus strobus seedlings: root disease pathogens and taxa associated with seed

    Science.gov (United States)

    C. M. Ocamb; J. Juzwik; F. B. Martin

    2002-01-01

    Eastern white pine (Pinus strobus L .) seeds were sown in soil infested wlth Fusarium proliferatum, root necrosis developed on seedling roots, and F. proliferatum as reisolated from symptomatic roots; thus, demonstrating that F. proliferatum is pathogenic to eastern white pine seedling. Soils...

  12. Assessing the gene content of the megagenome: sugar pine (Pinus lambertiana)

    Science.gov (United States)

    Daniel Gonzalez-Ibeas; Pedro J. Martinez-Garcia; Randi A. Famula; Annette Deflino-Mix; Kristian A. Stevens; Carol A. Loopstra; Charles H. Landley; David B. Neale; Jill L. Wegryzn

    2016-01-01

    Sugar pine (Pinus lambertiana Douglas) is within the subgenus Strobus with an estimated genome size of 31 Gbp. Transcriptomic resources are of particular interest in conifers due to the challenges presented in their megagenomes for gene identification. In this study, we present the first comprehensive survey of the P. lambertiana...

  13. Genetic conservation and management of the Californian endemic, Torrey Pine (Pinus torreyana Parry)

    Science.gov (United States)

    Jill A. Hamilton; Jessica W. Wright; F. Thomas. Ledig

    2017-01-01

    Torrey pine (Pinus torreyana) is one of the rarest pine species in the world. Restricted to one mainland and one island population in California, Torrey pine is a species of conservation concern under threat due to low population sizes, lack of genetic variation, and environmental stochasticity. Previous research points to a lack of within population variation that is...

  14. Influence of ammonia and ozone on growth and drought sensitivity of Pinus sylvestris

    NARCIS (Netherlands)

    Dueck, Th.A.; Zuin, A.; Elderson, J.

    1998-01-01

    Four-year-old Pinus sylvestris trees were exposed to ammonia (16, 55, 110 ppb for 24 h d-1)and ozone (0, 45 and 68 ppb, 9 h d-1) in a factorial design in open-top chambers for 15 months. Treatment effects on tree growth and architecture were assessed during two growing seasons; effects on

  15. Hydraulic redistribution of water from Pinus ponderosa trees to seedlings: evidence for an ectomycorrhizal pathway.

    Science.gov (United States)

    Jeffrey M. Warren; J. Renee Brooks; Frederick C. Meinzer; Joyce L. Eberhart

    2008-01-01

    Although there is strong evidence for hydraulic redistribution (HR) of soil water by trees, it is not known if common myconhizal networks (CMN) can facilitate HR from mature trees to seedlings under field conditions. Ponderosa pine (Pinus ponderosa) seedlings were planted into root-excluding 61-micron mesh barrier chambers buried in an old-growth...

  16. Geographic patterns of genetic variation and population structure in Pinus aristata, Rocky Mountain bristlecone pine

    Science.gov (United States)

    Pinus aristata Engelm., Rocky Mountain bristlecone pine, has a narrow core geographic and elevational distribution, occurs in disjunct populations and is threatened by multiple stresses, including rapid climate change, white pine blister rust, and bark beetles. Knowledge of genetic diversity and pop...

  17. Multiple nuclear loci reveal the distinctiveness of the threatened, Neotropical Pinus chiapensis

    Science.gov (United States)

    John Syring; Rafael F. del Castillo; Richard Cronn; Aaron Liston

    2007-01-01

    Pinus chiapensis is a threatened species of pine from southern Mexico and Guatemala. It was first described as a disjunct variety of P. strobus from the eastern United States and Canada. Subsequent morphological work indicates that P. chinpensis is a distinct species, but this interpretation is controversial. To...

  18. Chloroplast microsatellites reveal population genetic diversity in red pine, Pinus resinosa Ait

    Science.gov (United States)

    Craig S. Echt; L.L. DeVerno; M. Anzidei; G.G. Vendramin

    1998-01-01

    Variation in paternally inherited chloroplast microsatellite (cpSSR) DNA was used to study population genetic structure in red pine (Pinus resinosa Ait.), a species characterized by morphological uniformity, no allozyme variation, and limited RAPD variation. Using nine cpSSR loci, a total of 23 chloroplast haplotypes and 25 cpSSR alleles were were...

  19. Patterns of resistance to Cronartium ribicola in Pinus aristata, Rocky Mountain bristlecone pine

    Science.gov (United States)

    A. W. Schoettle; R. A. Sniezko; A. Kegley; R. Danchok; K. S. Burns

    2012-01-01

    The core distribution of Rocky Mountain bristlecone pine, Pinus aristata Engelm., extends from central Colorado into northern New Mexico, with a disjunct population on the San Francisco Peaks in northern Arizona. Populations are primarily at high elevations and often define the alpine treeline; however, the species can also be found in open mixed conifer stands with...

  20. Effects of fire season on vegetation in longleaf pine (Pinus palustris) forests

    Science.gov (United States)

    Bryan T. Mudder; G. Geoff Wang; Joan L. Walker; J. Drew Lanham; Ralph Costa

    2010-01-01

    Forest managers in the Southeastern United States are interested in the restoration of not only longleaf pine (Pinus palustris) trees, but also the characteristic forest structure and ground-layer vegetation of the longleaf pine ecosystem. Season of burn, fire intensity, and fire frequency are critical components of a fire regime that supports...

  1. Strength reduction in slash pine (Pinus elliotii) wood caused by decay fungi

    Science.gov (United States)

    Zhong Yang; Zhehui Jiang; Chung Y. Hse; Todd F. Shupe

    2009-01-01

    Small wood specimens selected from slash pine (Pinus elliotii )trees at three growth rates (fast, medium, and slow) were inoculated with brown-rot and white-rot fungi and then evaluated for work to maximum load (WML), modulus of rupture (MOR), and modulus of elasticity (MOE). The experimental variables studied included a brown-rot fungus (Gloeophyllum trabeum...

  2. Nuclear genetic variation across the range of ponderosa pine (Pinus ponderosa): Phylogeographic, taxonomic and conservation implications

    Science.gov (United States)

    Kevin M. Potter; Valerie D. Hipkins; Mary F. Mahalovich; Robert E. Means

    2015-01-01

    Ponderosa pine (Pinus ponderosa) is among the most broadly distributed conifer species of western North America, where it possesses considerable ecological, esthetic, and commercial value. It exhibits complicated patterns of morphological and genetic variation, suggesting that it may be in the process of differentiating into distinct regional...

  3. JUVENILE-MATURE GENETIC CORRELATIONS IN Pinus taeda CLONES PROPAGATED VIA SOMATIC EMBRYOGENESIS

    Directory of Open Access Journals (Sweden)

    Poliana Coqueiro Dias

    2016-04-01

    Full Text Available ABSTRACT This study aimed to estimate the genetic correlation among selection ages (juvenile - adult and efficiency of early selection for the height, diameter, and volume traits of individuals from Pinus taeda families propagated via somatic embryogenesis. This study was carried out by genetic-statistical analysis, estimation procedure of variance (Reml, and prediction components of breeding values (Blup, using the Selegen-Reml/Blup software. Genetic correlations among juvenile ages and rotation age were performed by applying the linear model developed by Lambeth (1980. In accordance with results of the established model, the early selection can be performed in clones of Pinus taeda with high selection efficiency. Ages from 4 to 6 years old are enough to select Pinus taeda clones propagated via somatic embryogenesis for harvesting at 8 and 12 years old; and 6 to 10 years old are enough to select them for harvesting at 20 years old. On the basis of the genetic correlations estimates from the environments, the clones' selection of Pinus taeda propagated via somatic embryogenesis should be developed specifically for each environment. The clones' selection can be performed considering the diameter due to the high correlation between volume and diameter.

  4. Evaluation of the heat pulse velocity method for measuring sap flow in Pinus patula

    CSIR Research Space (South Africa)

    Dye, PJ

    1996-07-01

    Full Text Available Information on the water use of Pinus patula plantations is required to predict the impact of forest plantations on water resources in South Africa, The heat pulse velocity (HPV) method is a promising technique for measuring water use by trees...

  5. Classification of Pinus patula, P. tecunumanii, P. oocarpa, P. caribaea var. hondurensis, and Related Taxonomic Entities

    Science.gov (United States)

    A.E. Squillace; Jesse P. Perry

    1992-01-01

    Stem xylem terpenes of 75 pine populations were studied to determine relationships among taxonomic entities. Typical Pinus patula populations occurring in areas north and west of Oaxaca, Mexico, had very high proportions of 3-phellandrene and low proportions of other constituents. Terpene compositions of populations of variety longipeduncalatain...

  6. Adjustments in hydraulic architecture of Pinus palustris maintain similar stomatal conductance in xerix and mesic habitats

    Science.gov (United States)

    R.N. Addington; L.A. Donovan; R.J. Mitchell; J.M. Vose; S.D. Pecot; S.B. Jack; U.G. Hacke; J.S. Sperry; R. Oren

    2006-01-01

    We investigated relationships between whole-tree hydranlic architecture and stomatal conductance in Pinus palustris Mill. (longleaf pine) across habitats that differed in soil properties and habitat structure. Trees occupying a xeric habitat (characterized by sandy, well-drained soils, higher nitrogen availability and lower overstory tree density)...

  7. Stand-level Allometry in Pinus taeda as Affected by Irrigation and Fertilization

    Science.gov (United States)

    J.S. King; T.J. Albaugh; H.L. Allen; L.W. Kress

    1999-01-01

    Changing environmental conditions have the potential to alter allometric relationships between plant parts, possibly leading to ecosystem-level feedbacks. We quantified allometric shifts in field-grown loblolly pine (Pinus taeda L.) in response to altered resource availability based on data from multiple harvests to correct for size-related changes...

  8. Seleção precoce de progênies de Pinus radiata a Sphaeropsis sapinea

    Directory of Open Access Journals (Sweden)

    Paula Rachel Rabelo Corrêa

    2012-06-01

    Full Text Available http://dx.doi.org/10.5902/198050985734Sphaeropsis sapinea é um importante patógeno para Pinus radiata, causando seca de ponteiros e morte de árvores, em plantios comerciais. Este estudo teve como objetivo selecionar progênies de Pinus radiata resistentes ao patógeno. Mudas de 16 progênies de Pinus radiata foram inoculadas com três isolados monospóricos do patógeno, em condição de casa de vegetação. Foram avaliados o comprimento das lesões e o grau de secamento dos ponteiros. Os resultados indicaram que a metodologia de seleção precoce foi adequada para selecionar progênies de Pinus radiata resistentes ao Sphaeropsis sapinea. A herdabilidade média das progênies variou de acordo com o isolado testado e o critério de seleção utilizado.

  9. Assessing longleaf pine (Pinus palustris) restoration after southern pine beetle kill using a compact experimental design

    Science.gov (United States)

    J.-P. Berrill; C.M. Dagley

    2010-01-01

    A compact experimental design and analysis is presented of longleaf pine (Pinus palustris) survival and growth in a restoration project in the Piedmont region of Georgia, USA. Longleaf pine seedlings were planted after salvage logging and broadcast burning in areas of catastrophic southern pine beetle (Dendroctonus frontalis) attacks on even-aged mixed pine-hardwood...

  10. The genetics of shortleaf pine (Pinus echinata mill.) with implications for restoration and management

    Science.gov (United States)

    John F. Stewart; Rodney E. Will; Barbara S. Crane; C. Dana Nelson

    2016-01-01

    Shortleaf pine (Pinus echinata Mill.) is an important commercial timber resource and forest ecosystem component in the southeastern USA. The species occurs in mainly drier sites as an early- to mid-successional species, is fireadapted, and it plays an important role in the fire ecology of the region. However, shortleaf pine genetics are not well-studied, especially in...

  11. Ectomycorrhizal fungi and Pinus sylvestris: aluminium toxicity, base cation deficiencies and exudation of organic anions.

    NARCIS (Netherlands)

    Schöll, van L.

    2006-01-01

    Keywords: aluminium (Al), base cations, BC:Alratio, magnesium (Mg), organic anions, oxalate, malonate, ectomycorrhizal fungi, Paxillus involutus, Pinus sylvestris (Scots pine)The finding of microscopic-small tunnels in mineral grains

  12. Effects of temperature on Pinus patula seedlings growing in pots in a ...

    African Journals Online (AJOL)

    The re-establishment of Pinus patula seedlings into sites with high harvesting residue (slash) loads can negatively affect the survival of these plants. Field trials have examined the role that insect pests and fungal diseases play in causing this phenomenon. Research has also indicated that temperatures at ground level tend ...

  13. Mortalidade em florestas de Pinus palustris causada por tempestade de raios

    Science.gov (United States)

    Kenneth W. Outcalt; Jorge Paladino Corrêa de Lima; Jose Américo de Mello Filho

    2002-01-01

    The importance of lightning as an ignition source for the fire driven Pinus palustris ecosystem is widely recognized. Lightning also impacts this system on a smaller scale by causing individual tree mortality. The objective of this study was to determine the level of mortality due to lightning activity at the Department of Energy's Savannah...

  14. Ostryopsis davidiana seedlings inoculated with ectomycorrhizal fungi facilitate formation of mycorrhizae on Pinus tabulaeformis seedlings

    Science.gov (United States)

    Shu-Lan Bai; Guo-Lei Li; Yong Liu; R. Kasten Dumroese; Rui-Heng Lv

    2009-01-01

    Reforestation in China is important for reversing anthropogenic activities that degrade the environment. Pinus tabulaeformis is desired for these activities, but survival and growth of seedlings can be hampered by lack of ectomycorrhizae. When outplanted in association with Ostryopsis davidiana plants on reforestation sites, P. tabulaeformis seedlings become...

  15. Two centuries of fire in a southwestern Virginia Pinus pungens community

    Science.gov (United States)

    E. K. Sutherland; H. Grissino-Mayer; C. A. Woodhouse; W. W. Covington; S. Horn; L. Huckaby; R. Kerr; J. Kush; M. Moore; T. Plumb

    1995-01-01

    Fire exclusion in fire-dependent forest communities can alter stand structure and composition. The objective was to construct a fire history of two Pinus pungens Lamb. communities growing in southwestern Virgina. Treering analysis of fire-scarred P. pungens specimens and a tree survey were used to determine species composition and age distributions. From 1798-1944,...

  16. Fossils matter: improved estimates of divergence times in Pinus reveal older diversification.

    Science.gov (United States)

    Saladin, Bianca; Leslie, Andrew B; Wüest, Rafael O; Litsios, Glenn; Conti, Elena; Salamin, Nicolas; Zimmermann, Niklaus E

    2017-04-04

    The taxonomy of pines (genus Pinus) is widely accepted and a robust gene tree based on entire plastome sequences exists. However, there is a large discrepancy in estimated divergence times of major pine clades among existing studies, mainly due to differences in fossil placement and dating methods used. We currently lack a dated molecular phylogeny that makes use of the rich pine fossil record, and this study is the first to estimate the divergence dates of pines based on a large number of fossils (21) evenly distributed across all major clades, in combination with applying both node and tip dating methods. We present a range of molecular phylogenetic trees of Pinus generated within a Bayesian framework. We find the origin of crown Pinus is likely up to 30 Myr older (Early Cretaceous) than inferred in most previous studies (Late Cretaceous) and propose generally older divergence times for major clades within Pinus than previously thought. Our age estimates vary significantly between the different dating approaches, but the results generally agree on older divergence times. We present a revised list of 21 fossils that are suitable to use in dating or comparative analyses of pines. Reliable estimates of divergence times in pines are essential if we are to link diversification processes and functional adaptation of this genus to geological events or to changing climates. In addition to older divergence times in Pinus, our results also indicate that node age estimates in pines depend on dating approaches and the specific fossil sets used, reflecting inherent differences in various dating approaches. The sets of dated phylogenetic trees of pines presented here provide a way to account for uncertainties in age estimations when applying comparative phylogenetic methods.

  17. The seed bank in Pinus stand regeneration in NW Spain after wildfire

    Directory of Open Access Journals (Sweden)

    L. Calvo

    2001-05-01

    Full Text Available In the Cantabrian area (northwest Spain Pinus stands occupy many of the original shrub communities that have been considered unproductive. These Pinus stands represent the vegetation which is most affected by fire. Regeneration after fire may occur in different ways: by resprouting or by germination or both. Germination was the only regeneration mechanism in Pinus species that appeared in these areas. The aim of this study is to determine the role of the soil seed bank in regeneration in this type of ecosystem. In order to carry out the study, three communities dominated by Pinus sylvestris which had suffered wildfires were chosen. In each of the three experimental sites of Pinus sylvestris stands the seed bank composition and above-ground vegetation were studied. The results allowed three species groups in the seed bank to be differentiated: those favoured by fire, amongst which some hardseeds, mainly belonging to Cistaceae and Leguminosae, were found; another group formed by outsider or opportunist species from outside the community and which used anemochory as their main dispersion mechanism; and the third group formed by those negatively affected, amongst which were species using vegetative resprout as the main regeneration mechanism. The species of greatest quantitative importance in the seed bank was Erica australis. In general, anemochorous species were predominant in the soil seed bank. During the first stages of succession chamaephytes were dominant and in the two years after fire therophytes were. No great similarity was observed between the bank composition and field vegetation from a qualitative viewpoint, due to differences in the presence of seeds of outsider plants in the bank and to the significance of the resprouting species in the field.

  18. Efecto in vitro de aceites esenciales de tres especies de Lippia sobre Moniliophthora roreri (Cif. y Par. Evans et al., agente causante de la moniliasis del cacao (Theobroma cacao L. In vitro effect of essential oils of three Lippia species on Moniliophthora roreri (Cif. and Par. Evans et al., causative agent of moniliasis of cocoa (Theobroma cacao L.

    Directory of Open Access Journals (Sweden)

    Betty Stefany Lozada

    2012-04-01

    Full Text Available Se evaluó el efecto antifúngico in vitro de cinco aceites esenciales (AEs (AE1, AE2, AE3, AE4 y AE5 extraídos de Lippia origanoides, L. citriodora y L. alba sobre aislados de monilia (Moniliophthora roreri obtenidos de frutos de cacao infectados provenientes de San Vicente de Chucurí, Santander, Colombia. Las plantas de Lippia fueron colectadas en cinco localidades colombianas. Los aislados de monilia (M1, M2, M3, M4 y M5 fueron caracterizados por su morfología, germinación y crecimiento en medios de cultivo. La actividad antifúngica de diferentes concentraciones de los AEs fue evaluada contra el aislado M2 y la cepa de M. roreri (ATCC 64239, para determinar su efecto sobre la germinación y la inhibición del crecimiento micelial. Los AEs estudiados inhibieron 100% de la germinación y del crecimiento micelial cuando fueron utilizados en concentraciones de 800 - 1000 µg/ml. Concentraciones de 200 µg/ml también mostraron efecto sobre los aislamientos fúngicos, siendo los AEs obtenidos de L. origanoides (AE2 y AE3 los más activos. Estos estaban compuestos principalmente por timol, p-cimeno, g-terpineno, acetato de timilo, carvacrol, b-mirceno, trans-b-cariofileno. Diferencias significativas (P The in vitro antifungal effect of five essential oils (EOs (EO1, EO2, EO3, EO4 and EO5 extracted from Lippia origanoides, L. citriodora and L. alba on isolates of Monilia (Moniliophthora roreri was evaluated. Lippia plants were collected at five locations in Colombia, and monilia isolates were obtained from infected cocoa fruits collected in San Vicente de Chucurí, Santander, Colombia. The fungi strains (M1, M2, M3, M4 and M5 were characterized by morphology, germination and growth in culture media. Antifungal activity of different concentrations of EOs was evaluated against the M2 and the isolated strain of M. roreri (ATCC 64239 determining their effect on germination and mycelial growth inhibition. The five essential oils studied inhibited

  19. Comparative recruitment success of pine provenances (Pinus sylvestris, Pinus nigra) under simulated climate change in the Swiss Rhone valley

    Science.gov (United States)

    Richter, Sarah; Moser, Barbara; Ghazoul, Jaboury; Wohlgemuth, Thomas

    2010-05-01

    Low elevation Scots pine forests of European inner-alpine dry valleys may potentially disappear under continued climate warming, largely in response to increased warming and drought effects. In the upper Rhone valley, the driest region in Switzerland, increased Scots pine mortality in mature forest stands and sparse tree establishment after a large-scale forest fire already give evidence for ongoing climate change. Furthermore, vegetation models predict a decline of Scots pine (Pinus sylvestris) and Pubescent oak (Quercus pubescens) even under a moderate temperature increase of 2-3°C. A decline of tree species in the region may lead to a transition from forest to a steppe-like vegetation. Such a change is of considerable concern for both biodiversity and natural hazard protection. Although changing climate conditions affect all life stages of a tree, its most vulnerable stage is recruitment. We tested P. sylvestris and P. nigra seedlings to simulated temperature increase and water stress, using seeds from the upper Rhone valley, Switzerland (CH), and from Peñyagolosa, Spain (ES). The experiment was located outdoors at the bottom of the Rhone Valley. Treatments consisted of factorial combinations of 3 precipitation regimes (‘wet spring-wet summer', ‘dry spring-dry summer' and ‘wet spring-dry summer') and 3 soil heating levels (+0 °C, +2.5 °C, +5 °C). Automatically operated shelters intercepted natural rainfall and different precipitation regimes were simulated by manual irrigation. We found significantly lower germination rates under dry conditions compared to wet conditions, whereas soil temperature affected germination rates only for P. nigra and when elevated by 5°C. Contrastingly, an increase of soil temperatures by 2.5 °C already caused a substantial decrease of survival rates under both ‘dry spring-dry summer' and ‘wet spring-dry summer' conditions. Precipitation regime was more important for survival than temperature increase. Seasonality of

  20. Estudi dels mecanismes de mort cel·lular induïts per un model d’isquèmia cerebral in vitro: implicació dels antagonistes dels receptors de mortJosé Rodríguez Álvarez

    OpenAIRE

    Sánchez Opazo, Guillem

    2014-01-01

    L'ictus o accident cerebrovascular és la segona causa de mort en els països industrialitzats i constitueix la primera causa de discapacitat en adults. L'únic tractament aprovat en l'actualitat és el trombolític activador del plasminògen tissular (tPA), el qual només es pot aplicar en un nombre molt reduït de pacients i dintre d'una estreta finestra terapèutica. Els mecanismes de mort cel·lular en la isquèmia cerebral són amplis i venen provocats per l'interrupció del flux sanguini al cervell,...

  1. Reconstrucción paleoambiental del Pleistoceno Superior en la desembocadura del río Millars a través del análisis palinológico

    Directory of Open Access Journals (Sweden)

    Manuel Casas Gallego

    2011-10-01

    Full Text Available Se presentan los resultados del análisis palinológico efectuado sobre sedimentos del Pleistoceno superior en la desembocadura del río Millars, que han permitido detectar un episodio frío que interrumpió la vegetación presente en la zona y ejerció un efecto de debilitamiento sobre el caudal del río. La presencia de polen de arbustos termófilos constata la importancia del litoral castellonense como zona refugio durante el último máximo glacial. La interpretación paleoclimática está basada en la variación de los porcentajes de Pinus, Juniperus, arbustos mesotermófilos y elementos acuáticos.

  2. Association of Pinus banksiana Lamb. and Populus tremuloides Michx. seedling fine roots with Sistotrema brinkmannii (Bres.) J. Erikss. (Basidiomycotina)

    Science.gov (United States)

    Lynette R. Potvin; Dana L. Richter; Martin F. Jurgensen; R. Kasten. Dumroese

    2012-01-01

    Sistotrema brinkmannii (Bres.) J. Erikss. (Basidiomycotina, Hydanaceae), commonly regarded as a wood decay fungus, was consistently isolated from bareroot nursery Pinus banksiana Lamb. seedlings. S. brinkmannii was found in ectomycorrhizae formed by Thelephora terrestris Ehrh., ...

  3. Usage of the pruned log index for loblolly pine (Pinus taeda and slash pine (Pinus elliottii Aplicação do Índice de Tora Podada para Pinus taeda e Pinus elliottii

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    Denise Jeton Cardoso

    2010-10-01

    Full Text Available

    The assessment of the quality of clearwood produced in pruned plantations of pine is necessary, especially to set price and know the utilization potential. The pruned log index (PLI, index used in Chile and New Zealand to characterize the quality of the logs, is a function of measurable variables of each log as diameter with defects, diameter 1.3 m from the largest end and the ratio between the cylinder volume common to the entire length of the log and the scaling volume through the method Smalian. This study aims at evaluating the ITP usage for slash pine (Pinus elliottii logs at the age 24 years and loblolly pine (Pinus taeda at 19, harvested
    in the regions of Ibaiti, Paraná, and Itapeva, São Paulo. The PLI values did not exceed 2.3, which  indicates that there is little clearwood on the logs. This has been proven through the veneering results, in which the potentially clear volume in relation to the log volume ranged between 52% and 55%, but 10.3% at the maximum, was turned into clearwood veneer. The slicing procedure in the lathe proved to be suitable, since it allowed the diameter of the knotty core to be measured as soon as the knot came out. The PLI showed itself as applicable for the Brazilian conditions.

    doi: 10.4336/2010.pfb.30.62.119

  1. Pinus mugo Krummholz Dynamics During Concomitant Change in Pastoralism and Climate in the Central Apennines

    OpenAIRE

    Li Dai; Caterina Palombo; Hein van Gils; David G. Rossiter; Roberto Tognetti; Geping Luo

    2017-01-01

    The dynamics of Pinus mugo krummholz during concomitant change in pastoral land use and climate in central Italy since the mid-20th century was investigated. Krummholz dynamics were detected using sequential aerial photography and fitted to a logistic regression model with elevation, grazing, proximity to beech forest, and proximity to krummholz as explanatory variables. Dendrochronological series were correlated with temperature and precipitation and fitted to a linear model. During this per...

  2. Adaptation of stone pines Pinus sibirica Du Tour and Pinus koraiensis Siebold et Zucc. to various environmental factors in the testing sites

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    G. V. Kuznetsova

    2016-10-01

    Full Text Available Provenance trials of Pinus sibirica Du Tour and Pinus koraiensis Siebold et Zucc. (here after «studied species» were studied. In our study we assessed the growth parameters as well as anatomical and morphological parameters of the studied species corresponding to different provenances of their testing in the south of the Krasnoyarsk and Khabarovsk territories.We determined that the growth rate of trees corresponding to different provenances is determined not only by the inherited characteristics, but also by adaptation. At both experimental regions the offspring of trees corresponding to local provenance are clearly better adapted. Nevertheless, at Krasnoyarsk Krai provenance trials, we found that the phenotypic indicators and degree of preservation of the offspring of two Korean pine corresponding to Obluchensky and Chuguevsky provenances are at the same level as for the local Siberian pine. Tree rings widths have been measured for the Siberian pine corresponding to different provenances at both plantations. We conclude that at the Ermakovskoe plantation there is a positive impact of the environmental conditions on tree-ring width for Korean pine corresponding to different provenances, and in Khabarovsk Krai there is a negative impact of the environmental conditions on tree-ring width for the Siberian pine corresponding to different provenances.

  3. Spatial Genetic Structure within and among Seed Stands of Pinus engelmannii Carr. and Pinus leiophylla Schiede ex Schltdl. & Cham, in Durango, Mexico

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    María Elena Ortiz-Olivas

    2017-01-01

    Full Text Available Studies of spatial genetic structure (SGS are important because they offer detailed insights into historical demographic and evolutionary processes and provide important information regarding species conservation and management. Pinus engelmannii and P. leiophylla var. leiophylla are two important timber tree species in Mexico, covering about 2.5 and 1.9 million hectares, respectively. However, studies in relation to population genetics are unfortunately scant. The aim of this research was to use amplified fragment length polymorphisms (AFLP analysis to identify potential differences in spatial genetic structure within and among seven Pinus engelmannii and nine P. leiophylla var. leiophylla seed stands in Durango, Mexico. Within the 16 seed stands of the two tested pine species, no significant SGS was detected, although SGS was detected among the seed stands. We concluded that the collection of seed in only some seed stands should not significantly alter the degree of genetic differentiation within the (collected seed. Distances between seed orchards and pollen propagators of more than 24 km for P. engelmannii and 7 km for P. leiophylla may be sufficient to limit contamination. Finally, local seeds should be used for (reforestation.

  4. Differences in defence responses of Pinus contorta and Pinus banksiana to the mountain pine beetle fungal associate Grosmannia clavigera are affected by water deficit.

    Science.gov (United States)

    Arango-Velez, Adriana; El Kayal, Walid; Copeland, Charles C J; Zaharia, L Irina; Lusebrink, Inka; Cooke, Janice E K

    2016-04-01

    We tested the hypotheses that responses to the mountain pine beetle fungal associate Grosmannia clavigera will differ between the evolutionarily co-evolved host lodgepole pine (Pinus contorta var. latifolia) and the naïve host jack pine (Pinus banksiana) and that these responses will be influenced by water availability. G. clavigera inoculation resulted in more rapid stem lesion development in lodgepole than in jack pine; water deficit delayed lesion development in both species. Decreased hydraulic conductivity was observed in inoculated lodgepole pine seedlings, likely because of tracheid occlusion by fungal hyphae and/or metabolite accumulation. Drought but not inoculation significantly impacted bark abscisic acid levels. Jasmonic and salicylic acid were implicated in local and systemic responses of both species to G. clavigera, with salicylic acid appearing to play a greater role in jack pine response to G. clavigera than lodgepole pine. Water deficit increased constitutive levels and/or attenuated induced responses to G. clavigera for several monoterpenes in lodgepole but not jack pine. Instead, inoculation of well-watered but not water deficit jack pine resulted in a greater number of xylem resin ducts. These findings reveal mechanisms underlying differences in G. clavigera-induced responses between lodgepole and jack pine hosts, and how water availability modulates these responses. © 2015 John Wiley & Sons Ltd.

  5. Strenght of the glue line of edge glued panels of Pinus taeda made with different adhesives

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    Merielen de Carvalho Lopes

    2013-12-01

    Full Text Available The study compares the strength of the glue line of edge glued panels of Pinus taeda made with different industrial adhesives. Three types of adhesives (poly(vinyl acetate (PVAc, emulsion polymer isocyanate (EPI and polyurethane (PU glued in two side gluing orientation (radial and tangential and analyzed after the conditioning tests (dry and wet were analyzed. The 360 specimens were prepared for determining the shear strength of edge glued panels of Pinus taeda, and these were tested in a universal testing machine called EMIC. The shear strength of the glue line at dry condition was lower in the tangential side for the three types of adhesives analyzed. The PU adhesive showed the highest values of shear strength in the applied conditions, differing from PVAc and EPI adhesive when tested on dry condition and did not differ from the others adhesives when tested on wet condition. The highest percentage of wood failure was observed after the shear strength test at dry condition to EPI adhesive glued in the radial side. And in the wet condition, the EPI adhesive and PU adhesive glued in tangential side showed higher mean values of wood failure. Thus, it was possible to conclude that the strength of the glue line of Pinus taeda wood was influenced by the type of adhesive and side gluing orientation, and showed differently behavior according to the condition of use (wet or dry.

  6. Effects Of Drought Stress on Germination in Fourteen Provenances of Pinus Brutia Ten. Seeds in Turkey

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    Hakan Şevik

    2015-02-01

    Full Text Available Pinus brutia Ten., Red pine, known to be tough drought resistant pine specie, could effectively be used for afforestation of disturbed areas. It is of great interest for the afforestation in arid zones. Appropriate seed sources for the specific areas guarantees reforestation success. Away from its native areas Pinus brutia Ten. is planted for its ornamental value and timber production purposes. Selection of drought resistant provenances can very well increase the survival success. In this study, the effects of water potential on germination were studied in fourteen provenances of Pinus brutia Ten. from Turkey. Water potentials between 0 and -8 bars were obtained using polyethylene glycol 6000 (PEG-6000 solutions. Seeds were kept for 35 day at 20 ± 0.5°C. A decrease in water potential produced a marked reduction in germination percentage and germination value. As a result, significant variations between the provenances were found. It was determined that, under a -8 bar water stress, Isparta-Bucak and Mersin-Silifke, respectively corresponding to 58% and 57% of the control group, were the least water stress affected provenances.

  7. Experimental study of Pinus densiflora Siebold et Zuccarini on Hyperlipidemia and lipid in Rats

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    Kim, Dae-Hyun

    2007-02-01

    Full Text Available In order to study the effects of Pinus densiflora on hyperlipidemia and lipid in rats, we divided the rats into groups(Normal group, Control group and Sample group and perfomed the experimental research. Hyperlipidemia and lipid in rats were induced by high fat diets for 8weeks. The sample group was administerd the extract of Pinus densiflora for 14 days and control group was administerd equal dose of oral. And then we measured the amount of serum triglyceride, Total cholesterol, LDL-cholesterol, HDL-cholesterol, Free Fatty Acid, phospholipid, Insuline, Laptin, Body weight, epididymis fat weight & rate, epididymis fat cell, Cardiac Risk Factor(CRF. The results were as followers : 1. Sample Group showed decreasing effects on Total cholesterol, Trigylceride, LDL-cholesterol, and Phospholipid levels in serum and CRF significantly. 2. Sample Group showed increasing effects on HDL-cholesterol level in serum significantly. 3. Sample Group showed decreasing effects on Insuline in serum significantly. 4. Sample Group showed increasing effects on Laptin in serum significantly. 5. Sample Group showed decreasing effects on Body weight, epididymis fat weight & rate, epididymis fat cell significantly. According to the above results, Pinus densiflora showed significant decreasing effects on hyperlipidemia and lipid in rats, it is considered that it is appropriate to apply for hyperlipidemia, obesity.

  8. Dissipation of excess excitation energy of the needle leaves in Pinus trees during cold winters

    Science.gov (United States)

    Zhang, AO; Cui, Zhen-Hai; Yu, Jia-Lin; Hu, Zi-Ling; Ding, Rui; Ren, Da-Ming; Zhang, Li-Jun

    2016-12-01

    Photooxidative damage to the needle leaves of evergreen trees results from the absorption of excess excitation energy. Efficient dissipation of this energy is essential to prevent photodamage. In this study, we determined the fluorescence transients, absorption spectra, chlorophyll contents, chlorophyll a/ b ratios, and relative membrane permeabilities of needle leaves of Pinus koraiensis, Pinus tabulaeformis, and Pinus armandi in both cold winter and summer. We observed a dramatic decrease in the maximum fluorescence ( F m) and substantial absorption of light energy in winter leaves of all three species. The F m decline was not correlated with a decrease in light absorption or with changes in chlorophyll content and chlorophyll a/ b ratio. The results suggested that the winter leaves dissipated a large amount of excess energy as heat. Because the cold winter leaves had lost normal physiological function, the heat dissipation depended solely on changes in the photosystem II supercomplex rather than the xanthophyll cycle. These findings imply that more attention should be paid to heat dissipation via changes in the photosystem complex structure during the growing season.

  9. Mycorrhization of containerised Pinus nigra seedlings with Suillus granulatus under open field conditions

    Energy Technology Data Exchange (ETDEWEB)

    Lazarevic, J.; Keca, N.; Martinovie, A.

    2012-07-01

    Seedling mycorrhization acts as an efficient tool for improving the quality of seedlings. In this study, the effectiveness of Suillus granulatus, originating from Pinus heldreichii forests (Montenegro), to produce containerized ectomycorrhizal seedlings of autochthonous Pinus nigra in open field conditions was investigated. Spore (106, 107, 108) and vegetative (1:16, 1:8, 1:4) inoculation on ectomycorrhizal formation and seedling growth were tested. Spore and vegetative inoculums of autochthonous Pisolithus arhizus were used in the same trial as additional control treatments. The utilization of vegetative and spore inoculums of autochthonous S. granulatus has proven to be an effective method of obtaining containerized ectomycorrhizal P. nigra seedlings under open field conditions after 11 months. S. granulatus spore inoculations resulted in well developed ectomycorrhiza, decreasing the growth of the P. nigra seedlings in the first growing season. Mycelial inoculations resulted in slightly developed S. granulatus ectomycorrhiza, which increased the growth of the seedlings. Therefore, it would be feasible to use spore inocula of S. granulatus, with 10{sup 6} spores per plant, to produce ectomycorrhizal P. nigra plants on a large scale. Controlled mycorrhizal inoculation of seedlings is not a common practice in Montenegrin and Serbian nurseries; as such, the obtained results will contribute to the enhancement of nursery production of Pinus nigra and other conifers. This also could be assumed as a starting point for many further efforts and investigations with autochthonous fungal and plant material in this region. (Author) 47 refs.

  10. Antimicrobial activity of needle essential oil of Pinus peuce Griseb. (Pinaceae from Macedonian flora

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    Marija Karapandzova

    2012-03-01

    Full Text Available Chemical composition and antimicrobial activity of needle essential oil, obtained by hydrodistillation from wild Pinus peuce Griseb. (Pinaceae, growing on three different locations in R. Macedonia were investigated in period 2008/2009. Carried out GC/FID/MS analysis, one hundred and three constituents were identified belonging to the six different classes of components: monoterpene hydrocarbons, oxygenated monoterpenes, sesquiterpene hydrocarbons, oxygenated sesquiterpenes, diterpenes and other non-terpene components, representing 88.61/94.04% of the entire oil. The most abundant constituents were α-pinene (12.89/27.34%, β-pinene (6.16/13.13%, limonene + β-phellandrene (2.09/6.64% and bornyl acetate (2.92/11.67% as well as trans-(E-caryophyllene (4.63/7.13% and germacrene D (8.75/20.14%. Antimicrobial screening of Pinus peuce needle essential oil was made by hole-plate diffusion and broth dilution method against 13 bacterial isolates of Gram positive and Gram negative bacteria and one strain of Candida albicans. The most sensitive bacteria against tested Pinus peuce essential oils were Streptococcus pneumonia encompassing Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus agalactiae, Acinetobacter spp. and Streptococcus pyogenes. Minimal inhibitory concentrations (MICs of the oils ranged from 7.5- 62.5 µl/ml.

  11. Chloroplast evolution in the Pinus montezumae complex: a coalescent approach to hybridization.

    Science.gov (United States)

    Matos, J A; Schaal, B A

    2000-08-01

    This study addresses the evolutionary history of the chloroplast genomes of two closely related pine species, Pinus hartwegii Lindl. and P. montezumae Lamb (subsect. Ponderosae) using coalescent theory and some of the statistical tools that have been developed from it during the past two decades. Pinus hartwegii and P. montezumae are closely related species in the P. montezumae complex (subsect. Ponderosae) of Mexico and Central America. Pinus hartwegii is a high elevation species, whereas P. montezumae occurs at lower elevations. The two species occur on many of the same mountains throughout Mexico. A total of 350 individuals of P. hartwegii and P. montezumae were collected from Nevado de Colima (Jalisco), Cerro Potosí (Nuevo León), Iztaccihuatl/Popocatepetl (México), and Nevado de Toluca (México). The chloroplast genome of P. hartwegii and P. montezumae was mapped using eight restriction enzymes. Fifty-one different haplotypes were characterized; 38 of 160 restriction sites were polymorphic. Clades of most parsimoniously related chloroplast haplotypes are geographically localized and do not overlap in distribution, and the geographically localized clades of haplotypes include both P. hartwegii and P. montezumae. Some haplotypes in the clades occur in only one of the two species, whereas other haplotypes occur in both species. These data strongly suggest ancient and/or ongoing hybridization between P. hartwegii and P. montezumae and a shared chloroplast genome history within geographic regions of Mexico.

  12. Clinal differentiation and putative hybridization in a contact zone of Pinus ponderosa and P. arizonica (Pinaceae).

    Science.gov (United States)

    Epperson, B K; Telewski, F W; Plovanich-Jones, A E; Grimes, J E

    2001-06-01

    The widely distributed Pinus subsection Ponderosae is a species complex that has a transition zone among taxa in the southwestern United States. In southern Arizona and New Mexico at least two recognized taxa, Pinus ponderosa var. scopulorum and Pinus arizonica or P. ponderosa var. arizonica, are known to coexist in close proximity. In this study, we report the existence of populations where the taxa are sympatric. One of the key characteristics distinguishing taxa is the number of needles per fascicle; P. ponderosa typically has three, P. arizonica has five. We examined the spatial distribution of needle-number types in a belt transect that covers a transition zone from nearly pure three-needle types at the top of Mount Lemmon to five-needle types downslope, in the Santa Catalina Mountains, Arizona. The spatial distribution is inconsistent with there being both free interbreeding among types and selective neutrality of types. Trees with intermediate types, having combinations of three, four, and five needles and/or mean numbers of needles between 3.0 and 5.0, are spatially concentrated in the middle of the transition zone. The spatial distribution supports the occurrence of hybridization and introgression, and this is consistent with reported crossabilities of the types. The results suggest that selection is acting, either on needle number per se or on other traits of the ecotype with which it may be in linkage disequilibrium, to maintain the observed steep clinal differentiation.

  13. Soil water balance in different densities of Pinus taeda in Southern Brazil

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    Jorge Luiz Moretti Souza

    2016-04-01

    Full Text Available This study aimed to quantify and compare water balance components over the course of a year for different Pinus taeda planting densities in an oxisol in southern Brazil. This experiment was conducted on 6-year-old trees in a clay oxisol at the Monte Alegre Farm, a property of the Klabin Company. The experimental design was a randomized block with four replicates and five treatments with different amounts of soil coverage: T100 (100% coverage - standard planting coverage; (T75, 75; T50, 50; and T25, 25%, and; T0 (without cover - clearcutting. The soil water storage and actual evapotranspiration under non-standard conditions were determined in a weekly estimated soil water balance (SWB with measured components. By the end of the year, the treatments had not reached field capacity or wilting point storage. The average value of total downward drainage was 100.2 mm, and the highest values occurred in the T75 and T100 treatments. The lowest population density (T25 had the highest actual evapotranspiration (ETr, due to the growth of the remaining Pinus taeda trees. The highest evapotranspiration occurred in September, due to the resumption of Pinus taeda growth.

  14. Evaluación de la actividad antibacteriana in vitro del aceite esencial de hierba luisa (cymbopogon citratus (dc) stapf), poaceae en una formulación cosmética con finalidad antiacneíca.

    OpenAIRE

    Meza Angos, Katherine Lourdes; Vargas Duque, Gabriela Geoconda

    2013-01-01

    The present research had as objectives to evaluate the in vitro antibacterial activity of the essential oil of Lemongrass (Cymbopogon citratus) against Propionibacterium acnes, bacteria that causes acne, to make a lotion and to evaluate the effectiveness in vitro and in vivo of the lotion formulated from this oil. The essential oil obtained from the leaves of the selected species, using a hydrodistillation system with steam entrainment; with the oil obtained miscibility tests were performe...

  15. Distribution of chemical compartments of soil organic matter and c stocks of a cambisol from south Brazil as affected by Pinus afforestation

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    Henrique Cesar Almeida

    2012-01-01

    Full Text Available Distribution and stocks of soil organic matter (SOM compartments after Pinus monoculture introduction in a native pasture area of a Cambisol, Santa Catarina, Brazil, were investigated. Pinus introduction increased soil acidity, content of exchangeable Al+3 and diminished soil nutrients. Nevertheless, soil C stock increased in all humic fractions of the 0-5 cm layer after Pinus afforestation. In the subsurface, the vegetation change only promoted SOM redistribution from the NaOH-extractable humic substances to a less hydrophobic humin fraction. Under Pinus, soil organo-mineral interactions were relevant up to a 15 cm depth, while in pasture environment, this mechanism occurred mainly in the surface layer.

  16. Bioecology of the fungus Sphaeropsis sapinea dyko & Sutton - agents of pinus species decline

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    Milijašević Tanja

    2002-01-01

    Full Text Available Sphaeropsis sapinea is a cosmopolitan fungus, identified in more than 50 countries of the world, on all continents, but it is primarily the species of warm lands. It is also a polyphagous fungus recorded from 11 coniferous genera. The most endangered and the most frequent host plants are Pinus species - it occurs on 48 pine species, among which the most susceptible are Pinus Radiata, P. nigra, P. sylvestris, P. ponderosa, P. resinosa, P. mugo, P. pinaster and P. elliotti. The greatest damage is caused on the introduced Pinus species and on those cultivated in artificial plantations, shelterbelts and in urban environments. In Yugoslavia S. sapinea is widely distributed both in the continental and in the Mediterranean parts. It was identified from ten pine species and six hosts from other coniferous genera. By the study in our country, the new hosts of this fungus were detected - Pinus jeffrey, P. peuce and P. heldreichii. The most endangered species in our country is Austrian pine, both in urban environments, and in plantations The symptoms of the disease are bud wilt, curling, stunting and necrosis of current year shoots and needles, dieback of top shoots, parts of crown or tree tops, branch and stem bark canker, root collar rot on the young plants in nurseries and their dying. This fungus also prevents seed germination of Pinus species and causes blue sap stain of the freshly cut wood, although sap stain was also observed on standing trees. More rarely it causes root rot and crown wilt of Pinus species. The main symptoms of infection, both of young plants and older trees, are the dieback of current year shoots S. sapinea can penetrate through buds, bark of young shoots and needles. The critical time of infection is the period from mid April to mid May. Then infection mainly penetrates through the bark of young shoots, which results in their dying. Infection through the needles occurs mainly at the time of their sudden growth or during summer

  17. In vitro

    Science.gov (United States)

    Augustine, Dominic; Rao, Roopa S; Anbu, Jayaraman; Chidambara Murthy, K N

    2017-12-01

    The earthworm coelomic fluid (ECF) has shown proven antiproliferative effect against breast, liver, gastrointestinal, and brain cancer, but it is least explored in oral cancer. The present in vitro study is an attempt to investigate the antiproliferative activity of ECF on oral cancer cell line squamous cell carcinoma (SCC)-9. ECF was collected from the species Eudrilus eugeniae (EE), Eisenia foetida (EF), and Perionyx excavatus (PE) stored at -80°C. Percentage inhibition of ECF on squamous cell carcinoma-9 cells in vitro was recorded at 24 h. Protein estimation was done using Bradford protein assay validated by the biuret method. Cytotoxicity was tested at 2.5, 5, 10, 20, 40, and 80 μg/ml concentrations by 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay in SCC-9 cells in vitro . GraphPad Prism 7.0 software was used to calculate the inhibitory concentration (IC 50 ). Chi-square test was used to analyze the difference between samples. The test samples EE, EF, and PE inhibited the growth of SCC-9 cells significantly in a dose-dependent manner, and the IC 50 values were found to be 4.6, 44.69, and 5.27 μg/ml, respectively. The antiproliferative effect was found to be variable among the three earthworm species with EE showing the most promising effect followed by PE and EF. Establishing the antiproliferative effect of ECF on oral cancer cells could be an initial step toward drug development and future anticancer research. The preliminary investigation has shown that ECF has a promising antiproliferative effect on oral cancer cells in vitro . The present pilot study evaluated the in vitro antiproliferative effect of earthworm coelomic fluid (ECF) of Eudrilus eugeniae (EE), Eisenia foetida (EF), and Perionyx excavatus (PE) on squamous cell carcinoma-9 cell line. The ECF inhibitory activity was promising at inhibitory concentration values of 4.6, 44.69, and 5.27 μg/ml, respectively. Further studies pertaining to antiproliferative mechanism of EE

  18. BIODIVERSIDAD VEGETAL ASOCIADA A PLANTACIONES FORESTALES DE Pinus caribaea MORELET Y Eucalyptus pellita F. MUELL ESTABLECIDAS EN VILLANUEVA, CASANARE, COLOMBIA PLANT BIODIVERSITY ASSOCIATED TO FOREST PLANTATIONS WITH Pinus caribaea MORELET AND Eucalyptus pellita F. MUELL. ESTABLISHED IN VILLANUEVA, CASANARE, COLOMBIA

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    Fernando Fernández Méndez

    2012-06-01

    Full Text Available Resumen. Se analizó en una plantación ubicada en el bosque húmedo tropical la posibilidad que tienen las especies nativas de formar un sotobosque bajo plantaciones de Pinus caribaea y Eucalyptus pellita. Se establecieron parcelas permanentes en las plantaciones por especie, edad y tratamiento silvicultural; parcelas similares se instauraron en bosque natural y sabana. Se registraron individuos clasificados por tamaño y hábito. Se determinaron 49 familias botánicas y 102 especies. La mayor y menor diversidad se presentó en el bosque natural y en la sabana con 53 y 18 especies, respectivamente. Entre plantaciones, se encontró mayor diversidad en P. caribaea, con 46 especies, que en E. pellita con 38 especies. El cociente de mezcla indicó una vegetación heterogénea en todos los usos. Los índices Margalef y Menhinick mostraron que el bosque es más diverso, seguido de las plantaciones de mayor edad y por último la sabana. Los índices de Shannon y Simpson califican todos los sitios con vegetación heterogénea. Los tratamientos tuvieron diferencias estadísticas significativas en número de individuos, especies y categorías de tamaño, a excepción de las herbáceas. En cuanto a composición y abundancias se destacan tres grandes grupos: bosque con plantaciones maduras, plantaciones de edades intermedias y plantaciones jóvenes con sabana. Se observó una alta betadiversidad entre los tratamientos, que compartían menos del 50% de las especies y abundancias, según los índices Jaccard y Sorensen. Entre plantaciones se presentó el mayor número de especies compartidas. Se concluye que las plantaciones albergan buena cantidad de biodiversidad vegetal de sotoboque y no impiden el establecimiento de especies nativas.Abstract. It was analized in a plantation located in the tropical rainforest, the possibility that native species forming an understory in Pinus caribaea and Eucalyptus pellita plantations. Were established permanent plots

  19. Consideraciones paleoambientales del Tardiglaciar y Holoceno inicial en el Levante español: macrorrestos vegetales de El Tossal de la Roca (Vall d' Alcalà, Alicante

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    Uzquiano, Paloma

    1997-06-01

    Full Text Available Charred macrobotanical remains from El Tossal de la Roca permit a better understanding of the Tardiglacial-Postglacial (Upper Palaeolithic levels transition in Valencia region, showing evidence of a supramediterranean environment with Pinus nigra, Juniperus t. phoenicea and Quercus t. faginea. During the early Holocene (Epipaleolithic levels Quercus ilex-coccifera is dominant, followed by Quercus t. faginea.El análisis de los macrorrestos vegetales carbonizados procedentes del Tossal de la Roca ha permitido conocer la transición Tardiglaciar-Postglaciar (niveles del Paleolítico superior en la región de Valencia, constatando una vegetación de carácter supramediterráneo con Pinus sylvestris-nigra. Juniperus t. phoenicea y Quercus t. faginea. A inicios del Holoceno (niveles epipaleolíticos el taxon dominante es Quercus ilex seguido de Quercus t. faginea.

  20. Intra- and interpopulation relationships and taxonomic status of Pinus nigra Arnold in Croatia according to morphology and anatomy of needles

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    Zlatko Liber

    2014-01-01

    Full Text Available Intra- and interpopulational relationships and taxonomic status of six natural black pine populations (Pinus nigra Arnold from Croatia were analyzed. Special attention in this research was paid to the establishment of taxonomic status and distribution of stenoendemic Dalmatian black pine (Pinus nigra Arnold subsp. dalmatica (Vis. Franco. Three morphological and twenty-one anatomical traits of needles were used as well as analysis by the methods of multivariate statistics. Most of the researched individuals showed strong population affiliation, whereas a clinal transition of morphological and anatomical traits of needles was determined among the populations. The opinion of the existence of three subspecies of black pine on the territory of Republic of Croatia (Pinus nigra Arnold subsp. nigra, Pinus nigra Arnold subsp. dalmatica (Vis. Franco and Pinus nigra Arnold subsp. illyrica (Vid. Fukarek was confirmed. For each of the three subspecies new ranges were represented. Four Croatian populations were included in the stenoendemic subspecies of the Dalmatian black pine (Brač, Hvar, Grobnik and Pelješac, and a new distribution, broken in two, was suggested. Since the Dalmatian black pine has been reduced to several populations only, and there are some signs of continuous diminishing of its range, this research points out the need of making an action plan to preserve all natural populations of this subspecies.

  1. Efecto de la inclusión de un forraje tanífero sobre las poblaciones metanogénicas del ecosistema ruminal en condiciones in vitro e in vivo

    OpenAIRE

    Angarita Amaya, Erika Andrea

    2013-01-01

    La producción de metano en rumiantes está estrechamente ligada a la composición de los forrajes y metabolitos secundarios como taninos, mostrando efectos directos e indirectos sobre las poblaciones microbianas del rumen. El impacto de leguminosas taníferas sobre las poblaciones metanogénicas en condiciones del trópico de altura en Colombia, es desconocida a la fecha. Por lo anterior, este estudio buscó evaluar el efecto de la inclusión de la leguminosa tanífera Lotus uliginosus (Lotus) sobre ...

  2. DETERMINACIÓN DE LA CALIDAD NUTRITIVA, FERMENTACIÓN In Vitro Y METABOLITOS SECUNDARIOS EN ARVENSES Y RASTROJO DE MAÍZ UTILIZADOS PARA LA ALIMENTACIÓN DEL GANADO LECHERO

    OpenAIRE

    R. Martínez-Loperena

    2011-01-01

    En los sistemas campesinos del altiplano central mexicano en la época de lluvias existe una gran disponibilidad de recursos naturales forrajeros, tal es el caso de las arvenses (plantas que crecen dentro de los cultivos de maíz), que son ampliamente utilizadas para la alimentación del ganado lechero. El objetivo fue determinar la calidad nutritiva, metabolitos secundarios de las arvenses y el efecto que tienen en la cinética de fermentación ruminal al ser mezcladas con el rastrojo de maíz en ...

  3. In vitro

    Science.gov (United States)

    Zhao, Wei; Li, Jianke; He, Xiaoye; Lv, Ou; Cheng, Yujiang; Liu, Run

    2014-01-01

    This study was aimed to compare the relative activities of the purified pomegranate peels polyphenols (PPPs) with some other plant polyphenols including punicalagin, ellagic acid, gallic acid, phlorizin, and epigallocatechin gallate (EGCG) on the lipid metabolism regulation, and the cholesterol efflux mechanisms of PPPs and punicalagin were also investigated. In this paper, a convenient and accurate in vitro HL7702 steatosis hepatic cell model was applied to evaluate the lipid-lowering effects of the tested polyphenols. The results showed that PPPs possessed the strongest lipid-lowering effects. Prevention group (treated with polyphenols when establishing of steatosis model) was more effective than treatment group (treated with polyphenols after establishment of steatosis model). Punicalagin displayed the strongest lipid-lowering effects among all the tested components of pomegranate peel polyphenols. Moreover, PPPs and punicalagin (10, 20, 40 μg/mL) significantly increased the mRNA expression of LXRα (Liver X receptor alpha) and its target genes-ABCA1 (ATP-binding cassette transporter A1) in a dose-dependent manner in HL7702 steatosis hepatic cells. The high mRNA expression of LXRα and ABCA1, next to lovastatin, was observed in cells treated with 40 μg/mL of PPPs. These in vitro findings suggested that PPPs might have great potential in the clinic treatment of hyperlipemia.

  4. In vitro

    Science.gov (United States)

    Youssefi, M R; Abouhosseini Tabari, M; Moghadamnia, A A

    2017-01-01

    In Iranian folk medicine Artemisia sieberi has been used for treatment of parasite infections in human and animals. The present study was designed to evaluate the in vitro and in vivo effects of A. sieberi essential oil (EO) against Trichomonas gallinae . Trichomonas gallinae were recovered by wet mount method from infected native pigeons. The in vitro assays were accomplished in multi-well plates containing metronidazole (MTZ) as a standard antitrichomonal and EO in final concentrations of 2.5, 5, 10, 20, 50, and 100 μg/ml of culture medium containing 10 4 parasites. The in vivo assay was performed on 40 experimentally infected pigeons receiving 25 and 50 mg/kg of MTZ and EO for 7 successive days. Gas chromatographic (GC) analysis was performed to reveal chemical constituents of the EO. At 20 µg/ml, MTZ resulted in no viable trophozoite in culture medium after 24 h incubation period. While the 24 h MIC of EO was 10 µg/ml. Treatment with EO at dose of 50 mg/kg after 4 days led to full recovery of infected pigeons but for MTZ at the same dose 5 days were spent. Major constituents of EO were α-thujone (31.5%) and β-thujone (11.92%). Data of the present study introduced A. sieberi as a natural potent antitrichomonal agent effective against T. gallinae .

  5. The Influence of Pinus brutia on the Water Balance of Fractured Mediterranean Mountain Environments

    Science.gov (United States)

    Eliades, Marinos; Bruggeman, Adriana; Lubczynski, Maciek; Christou, Andreas

    2016-04-01

    In dry Mediterranean environments, both rainfall and temperature vary throughout the year and frequent droughts occur. The mountainous topography is characterized by steep slopes, often leading to shallow soil layers with limited water storage capacity. While for most of the tree species, these conditions can be characterized as unfavourable, Pinus brutia trees manage to survive and thrive. The main objective of this study is to define and quantify the water balance components of a Pinus brutia forest at tree level. Our study was conducted from 30/12/2014 until 31/09/2015 in an 8966-m2 fenced area of Pinus brutia forest. The site is located on the northern foothills of Troodos mountain at 620 m elevation, in Cyprus. The slope of the site ranged between 0 and 82%. The average daily minimum temperature is 5 0C in January and the average daily maximum temperature is 35 oC in August. The mean annual rainfall is 425 mm. We measured the diameter at breast height (DBH) from a total of 122 trees. Based on the average DBH, four trees were selected for monitoring (two were above the average DBH and two were below). We measured soil depth in a 1-m grid around each of the four selected trees. We processed soil depths in ArcGIS software (ESRI) to create a soil depth map. We used a Total Station and a differential GPS for the creation of a high resolution DEM of the area covering the four selected trees. We installed soil moisture sensors at 15-cm depth at distances of 1 and 2 m from the selected trees and a second sensor at 30-cm depth when the soil was deeper than 20 cm.. We randomly installed four metric manual rain gauges under each trees' canopy to measure throughfall and for stemflow we installed a plastic tube around each tree trunk and connected it to a manual rain gauge. We used six sap flow heat ratio method instruments to determine sap flow rates of the Pinus brutia trees. Two trees had one sensor installed at 1.3 m height facing north. The remaining trees had two sap

  6. Impact of Pinus Afforestation on Soil Chemical Attributes and Organic Matter in South Brazilian highlands

    Science.gov (United States)

    Pinheiro Dick, D.; Benvenuti Leite, S.; Dalmolin, R.; Almeida, H.; Knicker, H.; Martinazzo, R.

    2009-04-01

    The region known as Campos de Cima da Serra, located at 800 to 1400 m above sea level in the northeas of Rio Grande do Sul State, Brazil, is covered by a mosaic of natural grassland and Araucaria forest. Cattle raising, introduced by the first European settlers about 200 years ago, is the traditional economic activity in the region, occurring extensively and continuously on the natural pasture. In the last 30 years, while seeking for higher profits, local farmers have introduced agricultural crops and Pinus Taeda plantations in the original pasture lands. Pinus plantations are established in this area as dense monocultures and not as a sylvipastoral system, representing, thus, a severe threaten to the Campos' biodiversity. The soils are shallow, though very acidic (pH 4.2) and rich in exchangeable Al (28 to 47% of Al saturation), and present high contents of SOM in the surface layer (in general, higher than 4 %), which shows a low decomposition degree, as indicated by its high proportion of C-O alkyl groups (51 to 59 %). Considering that the biome sustainability of this region is being progressively affected by the change of land use and that systematic studies about exotic trees afforestation in that region are very scarce, our main objective was to investigate the impact of the introduction of Pinus on the SOM composition and chemical attributes of highland soils in 8 (Pi8) and 30 (Pi30) years old plantations, using as reference the original condition under native pasture (NP). In each studied Leptosol, soil samples were collected from three layers down to 15 cm ( 0-5 cm, 5-10 cm and 10-15 cm). Contents of exchangeable cations and of micronutrients and soil pH were determined. The SOM composition was investigated by means of elemental analyses, FTIR and fluorescence spectroscopy (three replicates). Prior to the spectroscopic analyses, samples were demineralized with 10% HF solution and organic matter loss was monitored. From the FTIR spectra, an aromaticity index

  7. Influencia de la selección de planta hospedadora, de los parasitoides y de la competencia intraespecífica en el desarrollo y mortalidad de la procesionaria del pino (Thaumetopoea pityocampa)

    OpenAIRE

    Pérez-Contreras, Tomás

    2013-01-01

    Se han estudiado diversos aspectos de la ecológia de la procesionaria del pino (Thaumetopoea pityocampa), un lepidóptero heterócero, de la familia Thaumetopoidaes, que constituye la principal plaga defoliadora de distintas especies de pino en el área mediterranea. El trabajo de campo se realizó durante el período 1993-1996, en un pinar de repoblación compuesto por pino de Alepo (pinus halepensis) y pino marítimo (pinus pinaster), localizado al este de la provincia de Granada. Debido a las dif...

  8. Drought Tolerance in Pinus halepensis Seed Sources As Identified by Distinctive Physiological and Molecular Markers

    Directory of Open Access Journals (Sweden)

    Khaled Taïbi

    2017-07-01

    Full Text Available Drought is one of the main constraints determining forest species growth, survival and productivity, and therefore one of the main limitations for reforestation or afforestation. The aim of this study is to characterize the drought response at the physiological and molecular level of different Pinus halepensis (common name Aleppo pine seed sources, previously characterized in field trials as drought-sensitive or drought-tolerant. This approach aims to identify different traits capable of predicting the ability of formerly uncharacterized seedlings to cope with drought stress. Gas-exchange, water potential, photosynthetic pigments, soluble sugars, free amino acids, glutathione and proteomic analyses were carried out on control and drought-stressed seedlings in greenhouse conditions. Gas-exchange determinations were also assessed in field-planted seedlings in order to validate the greenhouse experimental conditions. Drought-tolerant seed sources presented higher values of photosynthetic rates, water use efficiency, photosynthetic pigments and soluble carbohydrates concentrations. We observed the same pattern of variation of photosynthesis rate and maximal efficiency of PSII in field. Interestingly drought-tolerant seed sources exhibited increased levels of glutathione, methionine and cysteine. The proteomic profile of drought tolerant seedlings identified two heat shock proteins and an enzyme related to methionine biosynthesis that were not present in drought sensitive seedlings, pointing to the synthesis of sulfur amino acids as a limiting factor for drought tolerance in Pinus halepensis. Our results established physiological and molecular traits useful as distinctive markers to predict drought tolerance in Pinus halepensis provenances that could be reliably used in reforestation programs in drought prone areas.

  9. Drought Tolerance in Pinus halepensis Seed Sources As Identified by Distinctive Physiological and Molecular Markers.

    Science.gov (United States)

    Taïbi, Khaled; Del Campo, Antonio D; Vilagrosa, Alberto; Bellés, José M; López-Gresa, María Pilar; Pla, Davinia; Calvete, Juan J; López-Nicolás, José M; Mulet, José M

    2017-01-01

    Drought is one of the main constraints determining forest species growth, survival and productivity, and therefore one of the main limitations for reforestation or afforestation. The aim of this study is to characterize the drought response at the physiological and molecular level of different Pinus halepensis (common name Aleppo pine) seed sources, previously characterized in field trials as drought-sensitive or drought-tolerant. This approach aims to identify different traits capable of predicting the ability of formerly uncharacterized seedlings to cope with drought stress. Gas-exchange, water potential, photosynthetic pigments, soluble sugars, free amino acids, glutathione and proteomic analyses were carried out on control and drought-stressed seedlings in greenhouse conditions. Gas-exchange determinations were also assessed in field-planted seedlings in order to validate the greenhouse experimental conditions. Drought-tolerant seed sources presented higher values of photosynthetic rates, water use efficiency, photosynthetic pigments and soluble carbohydrates concentrations. We observed the same pattern of variation of photosynthesis rate and maximal efficiency of PSII in field. Interestingly drought-tolerant seed sources exhibited increased levels of glutathione, methionine and cysteine. The proteomic profile of drought tolerant seedlings identified two heat shock proteins and an enzyme related to methionine biosynthesis that were not present in drought sensitive seedlings, pointing to the synthesis of sulfur amino acids as a limiting factor for drought tolerance in Pinus halepensis. Our results established physiological and molecular traits useful as distinctive markers to predict drought tolerance in Pinus halepensis provenances that could be reliably used in reforestation programs in drought prone areas.

  10. Transcriptional analysis of Pinus sylvestris roots challenged with the ectomycorrhizal fungus Laccaria bicolor

    Science.gov (United States)

    Heller, Gregory; Adomas, Aleksandra; Li, Guosheng; Osborne, Jason; van Zyl, Len; Sederoff, Ron; Finlay, Roger D; Stenlid, Jan; Asiegbu, Frederick O

    2008-01-01

    Background Symbiotic ectomycorrhizal associations of fungi with forest trees play important and economically significant roles in the nutrition, growth and health of boreal forest trees, as well as in nutrient cycling. The ecology and physiology of ectomycorrhizal associations with Pinus sp are very well documented but very little is known about the molecular mechanisms behind these mutualistic interactions with gymnosperms as compared to angiosperms. Results Using a micro-array approach, the relative abundance of 2109 EST transcripts during interaction of Pinus sylvestris roots with the ectomycorrhizal fungus was profiled. The results reveal significant differential expression of a total of 236 ESTs, 96 transcripts differentially abundant after 1 day of physical contact with the fungus, 134 transcripts after 5 days and only 6 after 15 days at early stages of mantle formation on emerging lateral roots. A subset of cell wall modification and stress related genes was further assessed by quantitative reverse transcription PCR at late stages of mycorrhizal development coinciding with Hartig net formation. The results reveal down regulation of gene transcripts involved in general defence mechanism (e.g. antimicrobial peptide) as well as those involved in cell wall modification (e.g. glycine rich protein, xyloglucan endo transglycosylase). Conclusion This study constitutes the first attempt to characterize the transcriptome of the plant partner in the Pinus sylvestris – Laccaria bicolor model system. We identified 236 ESTs which are potentially important for molecular regulation of a functional symbiotic association in conifer host. The results highlight similarities with other studies based on angiosperm model systems, nevertheless some differences were found in the timing and spatial scale of gene regulation during ectomycorrhiza development in gymnosperms. The present study has identified a number of potentially important molecular events responsible for the

  11. Molecular phylogenetics and evolutionary history of sect. Quinquefoliae (Pinus): implications for Northern Hemisphere biogeography.

    Science.gov (United States)

    Hao, Zhen-Zhen; Liu, Yan-Yan; Nazaire, Mare; Wei, Xiao-Xin; Wang, Xiao-Quan

    2015-06-01

    Climatic changes and tectonic events in the Cenozoic have greatly influenced the evolution and geographic distribution of the temperate flora. Such consequences should be most evident in plant groups that are ancient, widespread, and diverse. As one of the most widespread genera of trees, Pinus provides a good model for investigating the history of species diversification and biogeographic disjunction in the Northern Hemisphere. In this study, we reconstructed the phylogeny and investigated the evolutionary and biogeographic history of sect. Quinquefoliae (Pinus), a species-rich lineage disjunctly distributed in Asia, Europe and North America, based on complete taxon sampling and by using nine DNA fragments from chloroplast (cp), mitochondrial (mt) and nuclear genomes. The monophyly of the three subsections, Krempfianae, Gerardianae, and Strobus, is well-supported by cpDNA and nuclear gene phylogenies. However, neither subsect. Gerardianae nor subsect. Strobus forms a monophyletic group in the mtDNA phylogeny, in which sect. Quinquefoliae was divided into two major clades, one consisting of the North American and northeastern Asian species as well as the European P. peuce of subsect. Strobus, and the other comprising the remaining Eurasian species belonging to three subsections. The significant topological incongruence among the gene trees, in conjunction with divergence time estimation and ancestral area reconstruction, indicates that both ancient and relatively recent introgressive hybridization events occurred in the evolution of sect. Quinquefoliae, particularly in northeastern Asia and northwestern North America. In addition, the phylogenetic analysis suggests that the species of subsect. Strobus from subtropical eastern Asia and neighboring areas may have a single origin, although species non-monophyly is very widespread in the nuclear gene trees. Moreover, our study seems to support a Tethyan origin of sect. Quinquefoliae given the distributions and

  12. Transcriptional analysis of Pinus sylvestris roots challenged with the ectomycorrhizal fungus Laccaria bicolor

    Directory of Open Access Journals (Sweden)

    Sederoff Ron

    2008-02-01

    Full Text Available Abstract Background Symbiotic ectomycorrhizal associations of fungi with forest trees play important and economically significant roles in the nutrition, growth and health of boreal forest trees, as well as in nutrient cycling. The ecology and physiology of ectomycorrhizal associations with Pinus sp are very well documented but very little is known about the molecular mechanisms behind these mutualistic interactions with gymnosperms as compared to angiosperms. Results Using a micro-array approach, the relative abundance of 2109 EST transcripts during interaction of Pinus sylvestris roots with the ectomycorrhizal fungus was profiled. The results reveal significant differential expression of a total of 236 ESTs, 96 transcripts differentially abundant after 1 day of physical contact with the fungus, 134 transcripts after 5 days and only 6 after 15 days at early stages of mantle formation on emerging lateral roots. A subset of cell wall modification and stress related genes was further assessed by quantitative reverse transcription PCR at late stages of mycorrhizal development coinciding with Hartig net formation. The results reveal down regulation of gene transcripts involved in general defence mechanism (e.g. antimicrobial peptide as well as those involved in cell wall modification (e.g. glycine rich protein, xyloglucan endo transglycosylase. Conclusion This study constitutes the first attempt to characterize the transcriptome of the plant partner in the Pinus sylvestris – Laccaria bicolor model system. We identified 236 ESTs which are potentially important for molecular regulation of a functional symbiotic association in conifer host. The results highlight similarities with other studies based on angiosperm model systems, nevertheless some differences were found in the timing and spatial scale of gene regulation during ectomycorrhiza development in gymnosperms. The present study has identified a number of potentially important molecular events

  13. Interspecific phylogenetic analysis enhances intraspecific phylogeographical inference: a case study in Pinus lambertiana.

    Science.gov (United States)

    Liston, Aaron; Parker-Defeniks, Mariah; Syring, John V; Willyard, Ann; Cronn, Richard

    2007-09-01

    Pinus lambertiana (sugar pine) is an economically and ecologically important conifer with a 1600-km latitudinal range extending from Oregon, USA, to northern Baja California, Mexico. Like all North American white pines (subsect. Strobus), sugar pine is highly susceptible to white pine blister rust, a disease caused by the fungus Cronartium ribicola. We conducted a chloroplast DNA (cpDNA) survey of Pinus subsect. Strobus with comprehensive geographical sampling of P. lambertiana. Sequence analysis of 12 sugar pine individuals revealed strong geographical differentiation for two chloroplast haplotypes. A diagnostic restriction site survey of an additional 72 individuals demarcated a narrow 150-km contact zone in northeastern California. In the contact zone, maternal (megagametophtye) and paternal (embryo) haplotypes were identified in 31 single seeds, demonstrating bidirectional pollen flow extending beyond the range of maternal haplotypes. The frequencies of the Cr1 allele for white pine blister rust major gene resistance, previously determined for 41 seed zones, differ significantly among seed zones that are fixed for the alternate haplotypes, or contain a mixture of both haplotypes. Interspecific phylogenetic analysis reveals that the northern sugar pine haplotype belongs to a clade that includes Pinus albicaulis (whitebark pine) and all of the East Asian white pines. Furthermore, there is little cpDNA divergence between northern sugar pine and whitebark pine (dS = 0.00058). These results are consistent with a Pleistocene migration of whitebark pine into North America and subsequent chloroplast introgression from whitebark pine to sugar pine. This study demonstrates the importance of placing phylogeographical results in a broader phylogenetic context.

  14. Resistance to Fungal Diseases of the Siberian (Pinus sibirica Du Tour and Korean (Pinus koraiensis Siebold et Zucc. Stone Pines in the Provenance Trails at the South of Krasnoyarsk Territory

    Directory of Open Access Journals (Sweden)

    I. D. Grodnitskaya

    2014-06-01

    Full Text Available The reason of the epiphytoty in the provenance trials of the Siberian (Pinus sibirica Du Tour and Korean (Pinus koraiensis Siebold et Zucc. stone pines in the foothills of the Western Sayan (south of Krasnoyarsk territory was established. The cause of stone pine needles drying was disease – grey pine-leaf cast (grey Schutte. The causative agent was pathogenic fungus Lophodermella sulcigena. The Siberian stone pine trees of Tashtagolskii climate type were drying (fatally to the greatest extent (21 %. The populations of the Korean stone pine showed higher resistance to fungal infections comparing to the Siberian stone pine climate type.

  15. Prospects for the biological control of invasive Pinus species (Pinaceae) in South Africa

    CSIR Research Space (South Africa)

    Hoffmann, JH

    2011-09-01

    Full Text Available Zealand. Proceedings of the New Zealand Plant Protection Conference 51: 216?223. BROCKERHOFF, E.G., HOFFMANN, J.H. & ROQUES, A. 2004. Is biological control an option for the management of wilding pines (Pinus spp.) in New Zealand? In: Hill, R....L., Zydenbos, S.M.& Bezar, C.M. (Eds) Managing Wilding Conifers in New Zealand: Present and Future. 65?78. New Zealand Plant Protection Society, Christchurch. COUTINHO, T.A., STEENKAMP, E.T., MONGWAKETSI, K., WILMOT, M. & WINGFIELD, M.J. 2007. First...

  16. Essential oils composition of Pinus peuce Griseb. (Pinaceae growing on Pelister Mtn., Republic of Macedonia

    Directory of Open Access Journals (Sweden)

    Marija Karapandzova

    2011-06-01

    Full Text Available The composition of essential oils obtained by hydrodistillation from needles, from branches without needles and from branches with needles of Pinus peuce Griseb. (Pinaceae from Pelister Mtn. (R. Macedonia was analyzed by GC/FID/MS. One hundred and seven components (40 monoterpenes, 37 sesquiterpenes, 9 diterpenes and 21 other components - aliphatic and cyclic hydrocarbons; aliphatic alcohols, aldehydes, and acids; phenols and other oxygenated benzene derivates were identified. The most abundant constituents were terpene hydrocarbons, encompassing the monoterpenes: α-pinene, β-pinene, limonene + β-phellandrene and bornyl acetate and the sesquiterpenes: trans (E-caryophyllene and germacrene D.

  17. PLANTAS NODRIZA EN LA REFORESTACIÓN CON Pinus hartwegii Lindl.

    OpenAIRE

    A. Ramírez-Contreras; D. A. Rodríguez-Trejo

    2009-01-01

    En el Ajusco, Distrito Federal, fueron plantados Pinus hartwegii en cinco tratamientos de plantas nodriza: al noreste (NE) de Lupinus montanus, al NE de Penstemon gentianoides, entre Festuca tolucensis, entre F. tolucensis con chaponeo, y en pequeños claros naturales. Se evaluó supervivencia, diámetro, altura, biomasa y concentración de N, P y K a seis (temporada de sequía) y doce meses (lluvias) de establecida la plantación. Se midió la radiación fotosintéticamente activa (RFA) en los distin...

  18. Alternativas de mejora de Pinus pinaster Ait. en la zona interior de Galicia.

    OpenAIRE

    Mata Pombo, Raúl de la

    2012-01-01

    El pino marítimo (Pinus pinaster Ait.) se distribuye por una amplia variedad de ambientes en el SO de Europa, desde los típicos climas Mediterráneos hasta áreas en Francia y en el Norte de España con marcada influencia oceánica. Dentro de su área de distribución natural las poblaciones aparecen altamente diferenciadas, mostrando adaptaciones locales a las condiciones ambientales. Ambos climas se encuentran en Galicia, donde se pueden distinguir dos regiones bioclimáticas: la costera y la inte...

  19. Ultrastructure of paraquat-treated pine cells (Pinus elliottii Engelm. ) in suspension culture

    Energy Technology Data Exchange (ETDEWEB)

    Birchem, R.; Henk, W.G.; Brown, C.L.

    1979-01-01

    The ultrastructure of liquid suspension cultures of Pinus elliottii was studied, noting characteristics of dividing and senescent cells. The cultures were treated with 0.01, 0.1, 1.0 and 10.0 mg 1/sup -1/ paraquat, an herbicide which stimulates oleoresin synthesis and resinosis in the xylem of treated pine trees. The ultrastructural effects of the toxin were studied at each paraquat concentration over a period of 24 days. The ultrastructural observations are correlated with physiological studies in suspension culture and in living trees.

  20. Influência da intensidade do desbaste sobre o crescimento em altura de Pinus elliottii E.

    Directory of Open Access Journals (Sweden)

    Paulo Renato Schneider

    2009-09-01

    Full Text Available Normal 0 21 false false false MicrosoftInternetExplorer4 O presente trabalho estudou a influência da intensidade de desbaste sobre o desenvolvimento da altura média e dominante de povoamentos de Pinus elliottii E., submetidos  a  4 (quatro  tratamentos de desbastes 25, 50 e 75 % da área basal da testemunha sem desbaste, além da testemunha sem desbaste. Os resultados obtidos permitiram concluir que a intensidade do desbaste influi no desenvolvimento da altura média e não no da altura dominante.

  1. Interannual variations in needle and sapwood traits of Pinus edulis branches under an experimental drought

    DEFF Research Database (Denmark)

    Guerin, Marceau; Martin-Benito, Dario; von Arx, Georg

    2018-01-01

    In the southwestern USA, recent large-scale die-offs of conifers raise the question of their resilience and mortality under droughts. To date, little is known about the interannual structural response to droughts. We hypothesized that pinon pines (Pinus edulis) respond to drought by reducing...... the drop of leaf water potential in branches from year to year through needle morphological adjustments. We tested our hypothesis using a 7-year experiment in central New Mexico with three watering treatments (irrigated, normal, and rain exclusion). We analyzed how variation in evaporative structure...

  2. Pinus roxburghii plant needles as a three-season biomonitor for elemental air pollution monitoring along roadside

    Energy Technology Data Exchange (ETDEWEB)

    Daud, Muhammad; Wasim, Mohammad; Khalid, Nasir; Waheed, Shahida [Pakistan Institute of Nuclear Science and Technology, Islamabad (Pakistan). Chemistry Div.

    2015-07-01

    The present study deals with the determination of 36 elements in Pinus roxburghii plant needles to assess their potential as biomonitor for elemental air pollution monitoring. The elemental quantification was made by employing Instrumental Neutron Activation Analysis (INAA) and Atomic Absorption Spectrometric (AAS) techniques. The needles of Pinus roxburghii were collected in spring, summer and winter seasons from various sites in Islamabad. The method validation was performed by analyzing two certified reference materials i.e., Citrus leaves - NIST-SRM-1572 and IAEA-Soil-7. Pollution level was assessed by using three indicators: enrichment factor, pollution load index and average toxic element concentration. The results indicated that the needles of Pinus roxburghii plant had potential to monitor the extent of air pollution in the vicinity of high traffic areas.

  3. Expresión de las moléculas del complejo mayor de histocompatibilidad clases I y II en pacientes con tuberculosis: efecto de la infección i n vitro con Mycobacterium tuberculosis H37Rv

    Directory of Open Access Journals (Sweden)

    Luis Fernando Barrera

    2001-04-01

    Full Text Available

    Es ampliamente conocido que pacientes con tuberculosis (TB
    activa pueden presentar alteraciones en la respuesta inmunológica, incluyendo defectos en la presentación antigénica. Dado el papel crítico del IFNγ y de las moléculas clase II en la inmunidad antimicrobiana, una estrategia desarrollada por microorganismos intracelulares, incluyendo virus, protozoos y bacterias, para evadir la respuesta inmune del hospedero, es la inhibición de la expresión de las moléculas del CMH. La mayor parte de las evidencias obtenidas hasta la fecha indican que estas infecciones intracelulares conducen a diferentes alteraciones en la vía de transducción de señales mediada por Jak1,2-Stat1, las
    cuales se han asociado con una disminución en la expresión superficial del CMH.
    Evidencias obtenidas en nuestro laboratorio, utilizando la línea
    celular de macrófagos murinos B10R infectada con Mtb, sugiere que esta infección altera la vía de transduccíon de señales mediada por Jak- Stat, la cual a su vez conduce a la disminución en la expresión de CIITA, un coactivador esencial para la expresión del CMH. De otro lado, se ha observado que la presencia de TGFβ e IL-10 también puede conducir a una disminución en la expresión del CMH II, a través de mecanismos
    dependientes e independientes de la vía JAK-Stat. Dado que una fracción apreciable de pacientes con TB muestran niveles elevados de estas citoquinas, ellas pudieran participar en la disminución de la expresión de las moléculas del CMH observada en algunos pacientes.

     

  4. Identification of microorganisms of Phytophthora genre associated to Quercus sp. and Pinus sp. species in the provinces of Guatemala and Sacatepéquez

    Directory of Open Access Journals (Sweden)

    José Humberto Calderón Díaz

    2015-07-01

    Full Text Available The mean purpose of this research was to identify microorganism belonging to Phytophthora genera which are affecting mixed natural forests and nurseries of Pinus sp. and Quercus sp. These species have economic and social impact in provinces such as Guatemala and Sacatepéquez. Soil and vegetal tissue were used to isolate Phytophthora from natural forest of Guatemala, 45 Pinus sp. and 10 Quercus sp. were sampled and from nurseries 88 Pinus sp. and 11 Quercus sp. From Sacatepéquez province, from natural forest system were sampled 48 Pinus sp. and 15 Quercus sp. From nurseries were sampled 58 Pinus sp. and 25 Quercus sp. After processing the samples from soil and roots 13 were found positive to Phytophthora sp. in Pinus maximinoi, 10 from Guatemala and three from Sacatepéquez provinces. The culture of Phytophthora sp. on PDA produced two colonies without define form and five stoloniferous, five semipetaloid, one stelade type colonies. VP16 isolate was inoculated in five species of pine for pathogenicity test, causing high percentages of incidence and severity on Pinus caribaea, P. oocarpa, P. pseudostrobus and P. maximinoi and low rates of incidence and severity on Pinus tecunumanii.

  5. The role of geographical barriers in shaping long-lived Pinus greggii varieties with incomplete lineage sorting and high levels of gene flow

    OpenAIRE

    Aguirre-Dugua, Xitlali; Gernandt, David Sebastian; Moreno-Letelier, Alejandra

    2016-01-01

    This poster was presented in Evolution 2016 conference, held in Austin, TX. We present data on the application of the hyb-seq method to Pinus subsetion Australes. Based on 117 SNPs we evaluate the differentiation of Pinus greggii varieties in two disjunct regions on the Sierra Madre Oriental, in Mexico.

  6. Evaluación económica de cáscara de nuez como sustrato para producción de plántulas de Pinus patula Schl. et Cham. en vivero

    OpenAIRE

    O. Romero-Arenas; M. A. Damián; Hernández, I.; C. Parraguirre; M. Márquez; Huerta, M.

    2013-01-01

    Se evaluó la rentabilidad de un vivero forestal para la producción de plántulas de Pinus patula en la sustitución del sustrato turba, por la composta de cáscara de nuez de castilla en una superficie de 1,000 m2, ubicado en Zitlalcuautla Tetela de Ocampo, en el estado de Puebla. Se utilizaron los indicadores de evaluación económica: valor actual neto (van), relación beneficio- costo (B/C), relación beneficio-inversión neta (n/k) y tasa interna de retorno (tir) para realizar el análisis de rent...

  7. In vitro

    Science.gov (United States)

    Maierl, Mario; Jörger, Michael; Rosker, Patrik; Reisner, Andreas

    2015-01-20

    Biofilm formation on catheters is thought to contribute to persistence of catheter-associated urinary tract infections (CAUTI) which represent the most frequent nosocomial infections. Understanding of factors relevant for CAUTI pathogenesis and evaluation of new therapeutics or interference strategies requires a model system that mirrors the physico-chemical conditions prevailing in a catheterized human bladder. The described in vitro dynamic model of a catheterized bladder enables to emulate many of the characteristics of a catheterized human bladder albeit in the absence of a bladder epithelium. A minor modification compared to the original model system (Stickler, et al. , 1999) allows temperature maintenance of the top 10 cm of the catheter, thereby enabling reproducible monitoring of biofilm formation on the internal catheter surface.

  8. In vitro

    Science.gov (United States)

    LuIs, Henrique Soares; Luis, Luis Soares; Bernardo, Mário

    2016-01-01

    Mouthrinses are used, by many of our patients, as a complement to daily dental hygiene routine. The use of a toothbrush and an interproximal cleaning method may not be enough to control dental plaque. Essential oils and delmopinol mouth rinses are effective for the prevention of dental caries and gingivitis. To study the effect of an essential oil and a delmopinol mouth rinse on dental plaque bacteria, an in vitro study was developed. The objective of this study was to determine the antibacterial activity of an essential oil and a delmopinol mouth rinse on Streptococcus mutans, Lactobacilli, and aerobic and anaerobic dental plaque nonspecific bacteria. Samples of human dental plaque were collected from consenting participants and bacteria isolated. Disk-diffusion tests were performed to obtain the minimum concentration of the mouth rinses necessary to inhibit bacterial growth. The ability of the commercial mouth rinses to inhibit bacterial growth was studied in comparison to a positive control (0.2% chlorhexidine) and a negative laboratorial control (sterilized water). The minimum inhibitory concentration was found to be inferior to the commercial essential oils and delmopinol mouth rinses concentrations. Delmopinol and essential oils have significant antibacterial properties shown in vitro only for aerobic bacteria, and for S. mutans, Lactobacillus, and anaerobic bacteria, the results were not statistically significant. Essential oils and chlorhexidine are statistically similar and better than delmopinol for aerobic bacteria growth inhibition. For the other bacteria, essential oils and delmopinol are not statistically promising. Results show that essential oils only may help patients to maintain good oral health as a complement to daily brushing and interproximal cleaning.

  9. In Vitro

    Science.gov (United States)

    Brown-Elliott, Barbara A; Wallace, Richard J

    2017-12-01

    There is a paucity of efficacious antimicrobials (especially oral) against clinically relevant species of Nocardia To date, all species of Nocardia have been susceptible to linezolid, the first commercially available oxazolidinone. Tedizolid is a new oxazolidinone with previously reported improved in vitro and in vivo (intracellular) potency against multidrug-resistant strains of Mycobacterium sp. and Nocardia brasiliensis Using the current Clinical and Laboratory Standards Institute-recommended broth microdilution method, 101 isolates of Nocardia spp., including 29 Nocardia cyriacigeorgica , 17 Nocardia farcinica , 13 Nocardia nova complex, 21 Nocardia brasiliensis , 5 Nocardia pseudobrasiliensis , and 5 Nocardia wallacei isolates and 11 isolates of less common species, were tested for susceptibility to tedizolid and linezolid. For the most common clinically significant species of Nocardia , tedizolid MIC 50 values were 0.25 μg/ml for N. nova complex, N. brasiliensis , N. pseudobrasiliensis , and N. wallacei , compared to linezolid MIC 50 values of 1, 2, 0.5, and 1 μg/ml, respectively. Tedizolid and linezolid MIC 90 values were 2 μg/ml for N. nova complex and N. brasiliensis Tedizolid MIC 50 and MIC 90 values for both N. cyriacigeorgica and N. farcinica were 0.5 μg/ml and 1 μg/ml, respectively, compared to linezolid MIC 50 and MIC 90 values of 2 and 4 μg/ml, respectively. Based on MIC 90 values, this study showed that tedizolid was 2- to 3-fold more active than linezolid in vitro against most common species of Nocardia , with the exception of the N. nova complex and N. brasiliensis , for which values were the same. These results may warrant evaluation of tedizolid as a potential treatment option for Nocardia infections. Copyright © 2017 American Society for Microbiology.

  10. Potencial del Agua del suelo

    Directory of Open Access Journals (Sweden)

    Bustamante Heliodoro

    1986-12-01

    Full Text Available La energía potencial del agua presenta diferencias de un punto del suelo a otro; esas diferencias son las que originan el movimiento del agua de acuerdo a la tendencia universal de la materia en el sentido de moverse de donde la energía potencial es mayor a donde dicha energía es menor. En el suelo el agua en consecuencia se mueve hacia donde su energía decrece hasta lograr su estado de equilibrio. Se desprende entonces que la cantidad de energía potencial absoluta contenida en el agua, no es importante por sí misma, sino por su relación con la energía en diferentes lugares dentro del suelo. El concepto Potencial de agua del suelo es un criterio para esta energía.

  11. Cultivo de células de Schwann, un modelo del microambiente del sistema nervioso

    OpenAIRE

    Vilma C. Muñetón; Zayra V. Garavito; Hernán Hurtado

    1998-01-01

    Algunos aspectos de la fisiopatología del sistema nervioso periférico pueden ser ampliamente estudiados en un modelo celular in vitro, enriquecido en células de Schwann. La célula de Schwann como glía del sistema nervioso periférico produce la mielina responsable de la transmisión saltatoria del impulso, influye en la actividad neuronal y da soporte y protección axonal. A su vez es blanco de procesos que alteran la normalidad del sistema nervioso periférico como neuropatías congénitas y 10 de...

  12. Índice de sitio para Pinus chiapensis (Martínez Andresen, en los estados de Veracruz y Puebla, México

    Directory of Open Access Journals (Sweden)

    M. Rodríguez-Acosta

    2005-01-01

    Full Text Available Se realizó una estimación del índice de sitio para Pinus chiapensis (Martínez Andresen, en el bosque natural de los municipios de Atzalan, Veracruz, Tlatlauquitepec y Yaonáhuac, Puebla, México. La base de datos se generó con 31 árboles dominantes y codominantes utilizando la metodología de análisis troncal, para obtener un total de 358 pares de datos de edad-altura. Los modelos exponenciales: poli-anamórfico con cambio continuo y poli-anamórfico con cambio discreto; los cuales fueron ajustados con procedimientos no lineales utilizando el sistema de análisis estadístico SAS y se compararon en sus versiones polimórficas utilizando el método de la diferencia algebraica. Los modelos de Chapman-Richards, Payandeh y Wang, Schumacher, mostraron un ajuste aceptable obteniendo un pseudo coeficiente de determinación de 0.98, 0.98 y 0.96 respectivamente. Se construyeron curvas polimórficas con dos de los modelos seleccionados y se fijaron tres índices de sitio: 20, 25 y 30 m a una edad base de 25 años.

  13. TRABAJABILIDAD DE LA MADERA DE Pinus oaxacana Mirov. PROVENIENTE DE UNA PLANTACIÓN EN EL ESTADO DE MÉXICO

    Directory of Open Access Journals (Sweden)

    Roberto Machuca-Velasco

    2012-01-01

    Full Text Available La calidad de maquinado es diferente en cada especie, por tanto, es necesario conocer las características y propiedades que intervienen en este proceso, tales como; la estructura anatómica, contenido de humedad, densidad básica, velocidad de crecimiento, número de anillos de crecimiento por pulgada y dirección del grano. El objetivo de este trabajo fue determinar las características de los ensayos de cepillado, lijado, moldurado, torneado y barrenado en la madera de Pinus oaxacana Mirov. El material de estudio se obtuvo en la plantación localizada a un costado de la División de Ciencias Forestales de la Universidad Autónoma Chapingo. Los ensayos de maquinado, la evaluación de los defectos y clasificación se realizaron según la Norma ASTM-D 1666-87, con algunas modificaciones realizadas en función de la maquinaria y contenidos de humedad. En general, la madera de P. oaxacana fue clasificada como una especie con excelente trabajabilidad, exceptuando el ensayo de moldurado en el corte preliminar que fue clasificado como bueno. Este ensayo mejoró en el segundo corte, por lo tanto, la especie puede ser utilizada en la elaboración de productos con mayor valor agregado, por ejemplo; en puertas, ventanas, marcos, piezas torneadas, molduras, lambrín o recubrimientos y muebles en general.

  14. Preliminary assessment for conservation of Pinus cernua (Pinaceae with a brief synopsis of related taxa in eastern Indochina

    Directory of Open Access Journals (Sweden)

    L. V. Averyanov

    2015-05-01

    Full Text Available The assessment of a recently discovered Laotian-Vietnamese endemic pine – Pinus cernua (Pinaceae is presented including new information about species morphology, taxonomy, ecology, distribution, as well as a proposed identification of conservation status according to the IUCN Red List criteria. An annotated list of related pine species known from eastern Indochina also needed updated conservation assessments is provided as well. Four nomenclature combinations – Pinus dalatensis var. anemophila (Businský Aver., P. dalatensis var. procera (Businský Aver., P. wangii var. varifolia (Nan Li et Y.C. Zhang Aver. and P. wangii var. eremitana (Businský Aver. are proposed.

  15. Resistência das madeiras de pinus, cedro australiano e seus produtos derivados ao ataque de Cryptotermes brevis

    OpenAIRE

    Ribeiro, Marcelo Xisto; Bufalino, Lina; Mendes, Lourival Marin; Sá, Vania Aparecida de; Santos, Alexandre dos; Tonoli, Gustavo Henrique Denzin

    2014-01-01

    Neste trabalho, objetivou-se comparar a resistência das madeiras de cedro australiano (Toona ciliata) e pinus (Pinus sp.) e de painéis aglomerados produzidos com essas espécies ao ataque do cupim de madeira seca Cryptotermes brevis, bem como quantificar a mortalidade dos insetos. Foram colocados 30 pseudo-operários em placas de Petri de acrílico de 9,5 cm de diâmetro, contendo as amostras (1,5 x 1,5 x 0,5 cm) e folhas de algodão posicionadas sobre as tampas perfuradas umedecidas diariamente c...

  16. A Comparative Study on Turpentine Oils of Oleoresins of Pinus sylvestris L. from Three Districts of Denizli

    Directory of Open Access Journals (Sweden)

    İbrahim Tümen

    2010-10-01

    Full Text Available Oleoresin samples collected from Pinus sylvestris L. trees from Acıpayam, Çal and Çamlıbel, three different locations in Denizli-Turkey. The constituents of the turpentine oil, obtained by hydrodistillation of oleoresin of Pinus sylvestris L., were identified by GC-MS. Fifty four constituents were detected from the turpentine oil, which constituted about between 96.2% and 98.2% of the total amount. Major constituents of the oil were a-pinene, b-pinene, camphene, longifolene, D3-carene, limonene and b-caryophyllene

  17. Herbal composition of Cinnamomum cassia, Pinus densiflora, Curcuma longa and Glycyrrhiza glabra prevents atherosclerosis by upregulating p27 (Kip1) expression.

    Science.gov (United States)

    Lee, Jung-Jin; Lee, Ji-Hye; Cho, Won-Kyung; Han, Joo-Hui; Ma, Jin Yeul

    2016-07-28

    Kiom-18 is a novel composition of Cinnamomum cassia, Pinus densiflora, Curcuma longa and Glycyrrhiza glabra. Curcuma longa and Glycyrrhiza glabra, which are traditional medicines in Asia, have been reported to demonstrate preventive effects against atherosclerosis; however, they have not yet been developed into functional atherosclerosis treatments. We therefore studied the anti-atherosclerotic effects and possible molecular mechanisms of Kiom-18 using vascular smooth muscle cells (VSMCs). To assess the anti-proliferative effect of Kiom-18 in vitro, we performed thymidine incorporation, cell cycle progression, immunoblotting and immunofluorescence assays in VSMCs stimulated by platelet derived-growth factor (PDGF)-BB. In addition, we used LDLr knockout mice to identify the effects of Kiom-18 as a preliminary result in an atherosclerosis animal model. Kiom-18 inhibited platelet-derived growth factor (PDGF)-BB-stimulated-VSMC proliferation and DNA synthesis. Additionally, Kiom-18 arrested the cell cycle transition of G0/G1 stimulated by PDGF-BB and its cell cycle-related proteins. Correspondingly, the level of p27(kip1) expression was upregulated in the presence of the Kiom-18 extract. Moreover, in an atherosclerosis animal model of LDLr knockout mice, Kiom-18 extract showed a preventive effect for the formation of atherosclerotic plaque and suppressed body weight, fat weight, food treatment efficiency, neutrophil count, and triglyceride level. These results indicate that Kiom-18 exerts anti-atherosclerotic effects by inhibiting VSMC proliferation via G0/G1 arrest, which upregulates p27(Kip1) expression.

  18. Chemical Composition of Pinus roxburghii Bark Volatile Oil and Validation of Its Anti-Inflammatory Activity Using Molecular Modelling and Bleomycin-Induced Inflammation in Albino Mice

    Directory of Open Access Journals (Sweden)

    Rola M. Labib

    2017-08-01

    Full Text Available The chemical composition of Pinus roxburghii bark essential oil (PRO was qualitatively and quantitatively determined using GC/FID and GC/MS. The anti-inflammatory activity was assessed in vitro by evaluating the binding percentages on the cannabinoids and opioids receptors. Bleomycin (BLM-induced pulmonary inflammation in albino mice was adopted to assess PRO anti-inflammatory efficacy in vivo. In silico molecular modelling of its major components was performed on human glucocorticoids receptor (GR. Seventy-five components were identified in which longifolene (33.13% and palmitic acid (9.34% constituted the predominant components. No binding was observed on cannabinoid receptor type 1 (CB1, whereas mild binding was observed on cannabinoid receptor type 2 (CB2, delta, kappa, and mu receptors accounting for 2.9%, 6.9%, 10.9% and 22% binding. A significant in vivo activity was evidenced by reduction of the elevated malondialdehyde (MDA, nitric oxide (NO, myeloperoxidase (MPO, interleukin-6 (IL-6, and tumor necrosis factor-α (TNF-α levels by 55.56%, 55.66%, 64.64%, 58.85% and 77.78% with concomitant elevation of superoxide dismutase (SOD and catalase (CAT activities comparable to BLM-treated group at 100 mg/kg body weight. In silico studies showed that palmitic acid exerted the fittest binding. PRO could serve as a potent anti-inflammatory natural candidate that should be supported by further clinical trials.

  19. Actividad antifungica del quitosano y aceites esenciales sobre Rhizopus stolonifer Vuill., agente causal de la pudricion blanda del tomate

    National Research Council Canada - National Science Library

    Alvarado Hernandez, Alejandra Maria; Barrera Necha, Laura Leticia; Hernandez Lauzardo, Ana Niurka; Velazquez del Valle, Miguel Gerardo

    2011-01-01

    .... Actualmente se buscan alternativas naturales para el control de las pudriciones poscosecha. Se evaluo in vitro e in situ el efecto antifungico del quitosano y de los aceites esenciales de canela...

  20. Unique features of the loblolly pine (Pinus taeda L.) megagenome revealed through sequence annotation.

    Science.gov (United States)

    Wegrzyn, Jill L; Liechty, John D; Stevens, Kristian A; Wu, Le-Shin; Loopstra, Carol A; Vasquez-Gross, Hans A; Dougherty, William M; Lin, Brian Y; Zieve, Jacob J; Martínez-García, Pedro J; Holt, Carson; Yandell, Mark; Zimin, Aleksey V; Yorke, James A; Crepeau, Marc W; Puiu, Daniela; Salzberg, Steven L; Dejong, Pieter J; Mockaitis, Keithanne; Main, Doreen; Langley, Charles H; Neale, David B

    2014-03-01

    The largest genus in the conifer family Pinaceae is Pinus, with over 100 species. The size and complexity of their genomes (∼20-40 Gb, 2n = 24) have delayed the arrival of a well-annotated reference sequence. In this study, we present the annotation of the first whole-genome shotgun assembly of loblolly pine (Pinus taeda L.), which comprises 20.1 Gb of sequence. The MAKER-P annotation pipeline combined evidence-based alignments and ab initio predictions to generate 50,172 gene models, of which 15,653 are classified as high confidence. Clustering these gene models with 13 other plant species resulted in 20,646 gene families, of which 1554 are predicted to be unique to conifers. Among the conifer gene families, 159 are composed exclusively of loblolly pine members. The gene models for loblolly pine have the highest median and mean intron lengths of 24 fully sequenced plant genomes. Conifer genomes are full of repetitive DNA, with the most significant contributions from long-terminal-repeat retrotransposons. In depth analysis of the tandem and interspersed repetitive content yielded a combined estimate of 82%.