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Sample records for visceral canina nota

  1. Leishmaniose visceral canina no Rio de Janeiro - Brasil

    OpenAIRE

    Marzochi,Mauro Célio de A.; Sérgio Gomes Coutinho; Paulo Chagastelles Sabroza; Miguel Alves de Souza; Pelágio Parigot Medeiros de de Souza; Luciano Toledo; Rangel Filho,Francisco B.

    1985-01-01

    No recente e mais meridional foco endêmico de leishmaniose visceral do Brasil, associado à presença de Lutzomyia longipalpis e a infecção canina, os autores discutem a importância do cão doméstico como reservatório natural da infecção, assim como sumarizam os achados epidemiológicos, clínicos, parasitológicos e terapêuticos da doença canina observados no Município do Rio de Janeiro, Brasil.Based on the recent and most southernly endemic focus of visceral leishmaniasis in Brazil, associated wi...

  2. Patologias genitais associadas à leishmaniose visceral canina Genital pathologies associated with canine visceral leishmaniasis

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    Vinícius Vasconcelos Gomes de Oliveira

    2012-09-01

    Full Text Available A leishmaniose visceral canina (LVC é uma doença parasitária crônica causada por protozoários pertencentes ao gênero Leishmania. No Brasil, a transmissão se dá, principalmente, pela ação hematófaga de insetos vetores pertencentes à subfamília Phlebotominae, particularmente, a espécie Lutzomyia longipalpis. Todavia, a trasmissão vertical e venérea da LVC está presente. Os principais sinais clínicos observados nos animais acometidos pela LVC são linfoadenomegalia, dermatopatias, hepatoesplenomegalia, onicogrifose e oftalmopatias, contudo quadros atípicos podem ser observados, inclusive com o envolvimento do sistema genital. Dessa forma, o objetivo deste artigo é realizar revisão sobre as principais patologias genitais em cães machos e fêmeas com leishmaniose visceral (LV.The canine visceral leishmaniasis (CanL is a chronic parasitic disease caused by protozoa belonging to the genus Leishmania. In Brazil, the transmission occurs mainly by the action of blood-sucking insects belonging to the subfamily Phlebotominae, particularly the Lutzomyia longipalpis species. However, the venereal and vertical transmission of the CanL is present. The main clinical signs observed in animals affected by the CanL are lymphadenopathy, skin diseases, hepatosplenomegaly, onychogryphosis and ophthalmopathy, however atypical manifestations can be observed, including the involvement of the genital system. Thus, the aim of this paper is to review on the major pathologies in genital male and female dogs with visceral leishmaniasis (VL.

  3. Canine visceral leishmaniosis: a comparative analysis of the EIE-leishmaniose-visceral-canina-Bio-Manguinhos and the IFI-leishmaniose-visceral-canina-Bio-Manguinhos kits.

    Science.gov (United States)

    Lira, R A; Cavalcanti, M Paiva; Nakazawa, M; Ferreira, A G P; Silva, E D; Abath, F G C; Alves, L C; Souza, W V; Gomes, Y M

    2006-04-15

    This study evaluated the performance of the EIE-leishmaniose-visceral-canina-Bio-Manguinhos (EIE-LVC) kit and to compare it with that of the IFI-leishmaniose-visceral-canina-Bio-Manguinhos (IFI-LVC) kit. Four groups of dogs were studied: group 1 (G1), dogs with clinical signs indicative of CVL and testing positive for the parasite (n = 25); group 2 (G2), dogs with only a presumed diagnosis of CVL (n = 62); group 3 (G3), dogs that had never lived in an area where CVL is endemic and never received a blood transfusion (n = 16); group 4 (G4), dogs carrying other parasites: such as babesiosis (n = 4), ehrlichiosis (n = 6) and demodicosis (n = 1). G1 and G3 were used for the calculation of sensitivity and specificity, respectively. The EIE-LVC showed a sensitivity of 72% (IC 95%: 50.4-87.1%) and a specificity of 87.5% (IC 95%: 60.4-97.8%). The value of the kappa index was 0.975 (CI 95%: 0.926-1.024), which represents an excellent fit. For IFI-LVC, the sensitivity was 68.0% (CI 95%: 46.4-84.3%) and the specificity 87.5% (CI 95%: 60.4-97.8%). When the tests were conducted in parallel, sensitivity was 92.0% (CI 95%: 72.5-98.6%) and specificity 75.0% (CI 95%: 47.4-91.7%). However, when conducted consecutively, the tests showed a sensitivity of 48.0% (CI 95%: 28.3-68.2%) and a specificity of 100.0% (CI 95%: 75.9-99.4%). The analysis of clinically suspected dogs using IFI-LVC and EIE-LVC kits in parallel, revealed that 26/62 animals were positive. Cross-reaction was observed in a dog with demodicosis. These results lead to the following conclusions: (1) the performance of the EIE-LVC kit is not statistically different from the IFI-LVC and (2) the kits must be used in parallel if higher sensitivity is required, reducing the number of false-negative results.

  4. Citometria de fluxo no diagnóstico da leishmaniose visceral canina Flow cytometry used in canine visceral leishmaniasis diagnosis

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    A.V. Carvalho Neta

    2006-08-01

    Full Text Available Descreve-se a padronização de nova metodologia para detecção de anticorpos antiformas promastigotas fixadas de L. (L. chagasi, por citometria de fluxo (AAPF-IgG, sua aplicabilidade e desempenho na identificação de casos de leishmaniose visceral canina (LVC. Foram avaliados dois grupos de cães classificados pela reação de imunofluorescência indireta (RIFI, como: não reatores (NR, n=10 e reatores (R, n=50 dos quais foram coletadas amostras de sangue (soro para realização dos testes laboratoriais. Os resultados relacionados ao estabelecimento, aplicabilidade e desempenho da metodologia AAPF-IgG demonstraram que essa metodologia possibilita a identificação de uma região de reatividade diferencial entre cães NR e R, no soro diluído a 1:2048 e o valor de 20% de parasitos fluorescentes positivos (PPFP como ponto de corte entre resultados positivos e negativos, mostrando que a AAPF-IgG aplica-se na identificação de casos de LVC, possibilitando distinguir 96% de cães R como positivos e 100% de cães NR como negativos. Esses resultados em conjunto sugerem que a utilização da AAPF-IgG pode ser um novo instrumento para ensaios clínicos de diagnóstico sorológico da LVC.The current study evaluated the standardization of a new methodology for detection of anti-fixed L. (L. chagasi promastigote antibodies by flow cytometry (AAPF-IgG, as well its applicability and performance in the identification of cases of Canine Visceral Leishmaniasis (CVL. Two groups of dogs were classified by RIFI (gold standard as no reactors (NR, n=10 and reactors (R, n=50. Blood samples were collected and used for the laboratorial tests (RIFI and AAPF-IgG. The results showed that the new AAPF-IgG assay makes possible the identification of an area of differential reactivity between dogs NR and R at the dilution of 1:2048 and 20% of percentage of positive fluorescent parasite as the cut point among positive and negative results. The AAPF-IgG assay was able to

  5. Leishmaniose visceral canina em três bairros de Uruguaiana - RS | Canine visceral leishmaniasis in three districts of Uruguaiana - RS

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    Laura Ilarraz Massia

    2016-02-01

    Full Text Available Este estudo teve como objetivo descrever a situação da Leishmaniose visceral canina (LCV em Uruguaiana quanto aos casos confirmados a partir de 2009 e à existência de associação entre o perfil socioeconômico e nível de conhecimento da população sobre a enfermidade em três bairros do município. Apesar da maioria dos entrevistados informar possuir conhecimento sobre a LVC, observou-se dificuldade em adotar uma das medidas preconizadas para a prevenção da doença (poder comprar a coleira. Houve associação entre renda e conhecimento sobre LVC (p = 0,04, poder comprar a coleira (p = 0,00 e limpar o pátio (p = 0,01. Entretanto, não se observou associação entre esta variável e achar que existe tratamento (p = 0,14 ou possuir pátio cercado (p = 0,13. Verificou-se também associação entre grau de escolaridade e conhecimento sobre LVC, poder comprar a coleira e achar que existe tratamento (p = 0,00. As informações resultantes da pesquisa podem auxiliar na condução de políticas públicas para a prevenção e controle da LVC. ================================================= This study aimed to describe the situation of LVC in Uruguaiana about the confirmed cases from 2009 and the existence of association between socioeconomic status and people’s level of knowledge about the disease in three districts of the municipality. Although the majority of respondents report having knowledge of the LVC, it found it difficult to adopt one of the measures recommend for the prevention of disease (to buy the collar. There was an association between income and knowledge of LVC (p = 0.04, to buy the collar (p = 0.00 and clean the patio (p = 0.01. However, there was no association between this variable and find that no treatment (p = 0.14 or own fenced yard (p = 0.13. It was also found association between level of education and knowledge of LVC, to buy the collar and you think there is treatment (p = 0.00. The resulting research information

  6. Relato de caso autóctone de leishmaniose visceral canina na zona sul do município do Rio de Janeiro

    OpenAIRE

    Fabiano Borges Figueiredo; Carlos José de Lima Barbosa Filho; Edvar Yuri Pacheco Schubach; Sandro Antonio Pereira; Lílian Dias Nascimento; Maria de Fátima Madeira

    2010-01-01

    O Brasil enfrenta uma expansão e urbanização da leishmaniose visceral americana com casos humanos e caninos em várias cidades de grande porte. O presente relato descreve um caso de leishmaniose visceral canina autóctone em uma área não endêmica no município de Rio de Janeiro.Brazil is facing expansion and urbanization of American visceral leishmaniasis, with human and canine cases in several large-sized cities. This report describes an autochthonous case of canine visceral leishmaniasis in a ...

  7. Leishmaniose visceral canina em Maricá, Estado do Rio de Janeiro: relato do primeiro caso autóctone Canine visceral leishmaniasis in Maricá, State of Rio de Janeiro: first report of an autochthonous case

    OpenAIRE

    Cíntia Cristiane de Paula; Fabiano Borges Figueiredo; Rodrigo Caldas Menezes; Eliame Mouta-Confort; Alessandra Bogio; Maria de Fátima Madeira

    2009-01-01

    Leishmaniose visceral é uma zoonose de importância em Saúde Pública, onde os cães representam um dos maiores problemas. Este trabalho visa relatar o primeiro caso autóctone da leishmaniose visceral canina no município de Maricá, fornecendo elementos relacionados à distribuição geográfica de Leishmania (Leishmania) chagasi no Estado do Rio de Janeiro.Visceral leishmaniasis is a zoonosis of public health importance, and dogs represent one of the main problems. This paper describes the first aut...

  8. Seroprevalencia de leishmaniosis visceral canina en la comuna 8 de Neiva y en cuatro municipios de Huila, Colombia

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    José Fernández

    2006-10-01

    Full Text Available Introducción. La leishmaniosis visceral canina en áreas endémicas de Colombia se constituye en un factor de riesgo para la salud pública, dado el carácter zoonótico de la enfermedad. Durante el año 2004 se registraron 96 casos de leishmaniosis visceral humana en el país, de los cuales cinco correspondieron al departamento del Huila, zona de influencia del vector Lutzomyia longipalpis. Objetivo. Determinar la prevalencia de anticuerpos IgG contra Leishmania chagasi en sueros de caninos recolectados en los municipios de Rivera, Palermo, Villavieja, Algeciras y la comuna 8 de la ciudad de Neiva, Huila, Colombia. Materiales y métodos. Se realizó una encuesta epidemiológica a los propietarios de 610 caninos y previo consentimiento se llevó a cabo examen clínico general y recolección de muestra de sangre de cada una de las mascotas. Los sueros fueron analizados mediante el test de ELISA empleando como antígeno promastigotes de Leishmania chagasi, de la cepa MHOM/CO/84/CL044B. El análisis de datos empleó estadística descriptiva. Resultados. En la población canina 85% fueron mestizos, la edad promedio fue 2,5 años y 67,3% de los animales fueron machos. Al examen clínico las manifestaciones más frecuentes fueron: onicogrifosis 24,3%, linfadenitis 10% y lesiones cutáneas 5%. La frecuencia de anticuerpos IgG contra Leishmania chagasi en la comuna 8 de la ciudad de Neiva fue 28,1%, en Villavieja 28%, en Rivera 14,9%, en Palermo 10% y en Algeciras 5,1%. Adicionalmente, se observó un promedio de cinco personas convivientes por canino seropositivo. Conclusiones. Estos datos demuestran la exposición de los caninos que habitan los ambientes rurales y urbanos de las zonas en estudio a Leishmania chagasi y deben tenerse en cuenta para el fortalecimiento de los programas de control y prevención de esta zoonosis en el Sistema General de Seguridad Social en Salud.

  9. Outbreak of autochthonous canine visceral leishmaniasis in Santa Catarina, Brazil Surto autóctone de leishmaniose visceral canina no Estado de Santa Catarina

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    Mário Steindel

    2013-04-01

    Full Text Available The present study reports the first outbreak of autochthonous canine visceral leishmaniasis in Florianópolis, Santa Catarina, southern Brazil. Following the report of two cases of CVL, the Control Center of Zoonotic Diseases conducted a serological survey by ELISA and IFAT assays in seven districts of the Santa Catarina Island. Eleven seropositive dogs of autochthonous transmission were used in the present study. Infection by Leishmania sp. was confirmed by parasitological examination of bone marrow, liver, spleen and lymph nodes, culture in Schneider's medium and PCR. Leishmania sp. isolates were characterized by PCR-RFLP and hybridization with specific probes, allowing for the identification of Leishmania infantum. Autochthonous transmission of this disease in an area with high tourist traffic presents a major public health concern and signifies the emergence of an important zoonosis in southern Brazil. Therefore, the implementation of surveillance and control measures is imperative to prevent the spread of the disease among the canine population as well as transmission to the human population.O presente estudo relata o primeiro surto autóctone de leishmaniose visceral canina (LCV em Florianópolis, Santa Catarina, Brasil. Durante levantamento soro-epidemiológico realizado pelo Centro de Controle de Doenças Zoonóticas (CCZ envolvendo 2.124 cães, 29 (1,37% foram soropositivos para VL (ELISA + RIFI. Onze cães positivos por transmissão autóctone foram utilizados no presente estudo. A confirmação da infecção por Leishmania sp. foi realizada pelo exame parasitológico da medula óssea, fígado, baço e linfonodos, cultura em meio Schneider e PCR. Os isolados de Leishmania sp. foram caracterizados por PCR-RFLP e hibridação com sondas específicas, permitindo a identificação de Leishmania infantum. A transmissão autóctone da LCV em uma área com grande fluxo turístico como Florianópolis representa um preocupante risco à saúde p

  10. Leishmaniose visceral: estudo de flebotomíneos e infecção canina em Montes Claros, Minas Gerais

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    Monteiro Érika Michalsky

    2005-01-01

    Full Text Available A leishmaniose visceral no Brasil estava inicialmente associada a áreas rurais, mas devido às diversas alterações no ambiente como, desmatamentos, urbanização e intenso processo migratório, ocorreu a expansão das áreas endêmicas, levando à urbanização da doença, principalmente nas regiões Sudeste e Centro Oeste do país. No município de Montes Claros, situado ao norte de Minas Gerais, foi feito um estudo para verificação da situação da LV. No ano de 2002 foi realizado inquérito sorológico canino e no período de setembro de 2002 a agosto de 2003 foi feito levantamento entomológico, utilizando armadilhas luminosas de CDC. A prevalência da LV canina apresentou taxa média de infecção em torno de 5%. A fauna de flebotomíneos estimada foi de 16 espécies, totalizando 1043 exemplares. Lutzomyia longipalpis foi a espécie predominante com 74%, o que sugere a sua participação na transmissão de LV em Montes Claros.

  11. Epidemiologic surveillance of canine visceral leishmaniasis in the municipality of Recife, Pernambuco Vigilância epidemiológica de leishmaniose visceral canina no município de Recife, Pernambuco

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    Filipe Dantas-Torres

    2005-10-01

    Full Text Available This paper describes an epidemiologic investigation carried out in Imbiribeira district, where a canine visceral leishmaniasis case was recorded. Despite the absence of seropositive dogs and sand flies, these findings are not sufficient to discard the occurrence of a zoonotic cycle of visceral leishmaniasis in Recife.Este artigo descreve uma investigação epidemiológica realizada no bairro da Imbiribeira, onde fora registrado um caso de leishmaniose visceral canina. Apesar da ausência de cães soropositivos e de flebotomíneos, esses achados não são suficientes para descartar a ocorrência do ciclo zoonótico da leishmaniose visceral em Recife.

  12. Avaliação da leishmaniose visceral canina em Poxoréo, Estado do Mato Grosso, Brazil Canine visceral leishmaniasis evaluation in Poxoréo, Mato Grosso State, Brazil

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    Márcia Ávila A. de Azevedo

    2008-09-01

    Full Text Available O cão doméstico desempenha importante papel como reservatório na transmissão da leishmaniose visceral ao homem, zoonose de grande importância em saúde pública. Realizou-se avaliação epidemiológica da leishmaniose visceral em 1.112 cães domiciliados no município de Poxoréo, estado do Mato Grosso e observou-se prevalência de 7,8%. Observou-se ainda associação estatisticamente significativa entre a prevalência de leishmaniose visceral canina e as variáveis faixa etária, presença de sinais clínicos e presença de outra espécie animal co-habitando com os cães avaliados, tendo sido as galinhas mais freqüentemente observadas entre os animais soropositivos. O sexo, a coleta de lixo domiciliar bem como a renda familiar não apresentaram associação estatisticamente significativa com a prevalência da leishmaniose visceral canina. A análise dos resultados sugere que cães com idade superior a sete anos e a , presença de outra espécie animal co-habitando com os cães podem ser fatores de risco para a leishmaniose visceral canina.Dogs play an important role as reservoir in the domestic cycle of visceral leishmaniasis, a serious public health problem. An epidemiological survey in 1,112 dogs was conducted at the Municipality of Poxoréo State of Mato Grosso, Brazil, using indirect immunofluorescence antibody test where the prevalence was 7.8%. Significant association was found between prevalence of canine visceral leishmaniasis and age of the dogs. Clinical signs and presence of other animals in the backyard, like chicken being more likely associated with seropositivity. Gender, garbage collection in the residence and family financial income were not associated with visceral leishmaniasis prevalence. Analysis of the results suggests that dogs aging more than 7 years and presence of another animal species co-inhabiting with the dogs may be risk factors for canine visceral leishmaniasis.

  13. Uso de Peptídeo Sintético selecionado por Phage Display para o Imunodiagnóstico da Leishmaniose Visceral Canina

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    Christina Monerat Toledo Machado

    2005-01-01

    Neste trabalho foram selecionados três antígenos através da técnica de Phage Display para o imunodiagnóstico da Leishmaniose Visceral Canina. Estes antígenos tiveram os DNAs extraídos e seqüenciados. O clone que apresentou a maior reatividade frente ao pool de soros de cães com leishmaniose visceral foi sintetizado através da síntese química em membrana de celulose e na sua forma solúvel, purificado e sua massa confirmada através de espectrometria de massa. Este peptídeo (TTDDDKLKKTLTYRS) foi...

  14. Investigação de áreas de risco como metodologia complementar ao controle da leishmaniose visceral canina Investigation of risk areas as complemental methodology for the control of canine visceral leishmaniasis

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    Fred S. Julião

    2007-08-01

    Full Text Available Foram investigadas áreas de risco de leishmaniose visceral canina no município de Camaçari, Bahia. Um total de 278 cães distribuídos em 141 residências, pertencentes a 20 áreas de risco investigadas, foi examinado sorologicamente (ELISA. A soroprevalência geral foi 21,7% (56/258 depois da exclusão dos 20 cães usados no início do estudo para delimitar a área. Os resultados respectivos das análises univariada e multivariada dos fatores relacionados à infecção do cão por Leishmania chagasi, a captura e distribuição do vetor na área e a metodologia usada para localizar os focos caninos são discutidos.Risk areas of canine visceral leishmaniasis in the city of Camaçari, Bahia, Brazil, were investigated. A total of 278 dogs from 141 homes pertaining to 20 investigated risk areas was serologically screened (ELISA. The general seroprevalence was 21.7% (56/258 after exclusion of 20 dogs used at the beginning of the survey to limit the study area. The respective results of the univariated and multivariated analysis of factors related to infection of dogs by Leishmania chagasi, to vector distribu-tion pattern in the area and to the methodology used to localize the canine focuses are discussed.

  15. Spatial distribution of human and canine visceral leishmaniasis in Belo Horizonte, Minas Gerais State, Brasil, 1994-1997 Distribuição espacial da leishmaniose visceral humana e canina em Belo Horizonte, Minas Gerais, Brasil, 1994-1997

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    Cláudia Di Lorenzo Oliveira

    2001-10-01

    Full Text Available In this paper, we present spatial analysis of the association between all incidents cases of human Visceral Leishmaniasis and seropositive dogs, from 1994 to 1997 in Belo Horizonte, a large Brazilian city. We geocoded 158 human cases and 11,048 seropositive dogs and compared canine prevalence rates with Human Bayesian Incidence rates in the same areas. We also used Knox's test to evaluate the hypothesis of space-time clustering of human cases in the period. Additionally, we used Kernel's maps for seropositive dogs distribution and located the human cases in the resulting smooth maps. We concluded that human and dog rates are correlated. Also, the Visceral Leishmaniasis in Belo Horizonte spread quickly, but apart from the rates' magnitude, it has kept the same spatial pattern through time. We believe it is possible to use this technique to choose areas to implement control measures against Visceral Leishmaniasis in a more efficient way.Neste artigo, apresentamos uma análise espacial da associação entre todos os casos incidentes de leishmaniose visceral e em cães soropositivos ocorridos em Belo Horizonte no período de 1994 a 1997. Geocodificamos 158 casos humanos e 11.048 cães positivos, comparamos as taxas de prevalência canina por área e as taxas Bayesianas de incidência da doença humana nas mesmas áreas. Usamos o teste de Knox para testar a hipótese de cluster espaço temporal entre os casos humanos no período examinado. Adicionalmente, construímos Mapas de Kernel para cães soropositivos e sobrepusemos os casos humanos em quatro áreas. Os resultados apontam para correlação entre casos humanos e caninos. Além disso, a leishmaniose visceral espalhou-se rapidamente em Belo Horizonte, embora tenha mantido o mesmo padrão durante os anos analisados. Acreditamos ser possível o uso das técnicas empregadas para priorizar áreas onde as medidas de controle devem ser implementadas.

  16. Estudo de coorte em áreas de risco para leishmaniose visceral canina em municípios da Região Metropolitana de Salvador, Bahia, Brasil.

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    Fred S. Julião

    2007-03-01

    Full Text Available Este estudo objetivou investigar a incidência de leishmaniose visceral em populações caninas de áreas de risco para a doença previamente identificados nos municípios de Lauro de Freitas e Camaçari, Bahia e estimar a associação entre variáveis de risco e a soroconversão de cães tidos como soronegativos em estudo prévio. Cerca de nove a 18 meses foi o intervalo entre a primeira sorologia (estudo prévio e os resultados descritos neste trabalho. Das 20 áreas de risco reexaminadas, foram testadas em enzime-linked immunosorbent assay amostras de 147 cães e aplicado simultaneamente um questionário epidemiológico aos respectivos proprietários. A incidência geral encontrada foi de 18,4% (27/147, sendo 17,4% (4/23 em Lauro de Freitas e 18,5% em Camaçari (23/124. A presença de galinha e suínos no peridomicílio e registro de cão com leishmaniose visceral eliminado na vizinhança resultaram em incremento do risco relativo de infecção dos cães por Leishmania sp. A eutanásia dos cães previamente detectados como soropositivos, não contribuiu efetivamente para a eliminação da transmissão do parasito nas áreas de risco. Estes dados demonstram que estas áreas permaneceram com transmissão ativa do parasito mesmo após a retirada, ainda que incompleta no município de Camaçari, dos cães soropositivos, e discute a participação de alguns fatores de risco na manutenção da endemicidade nestas áreas.

  17. Surveillance of canine visceral leishmaniasis in a disease-free area Vigilância da leishmaniose visceral canina em área indene

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    Michele Salmon Frehse

    2010-03-01

    Full Text Available Leishmaniasis is an important re-emergent parasitosis worldwide, particularly in tropical countries. There are no reports of autochthonous disease in the State of Paraná, southern Brazil. No surveillance has been carried out in the most populated areas such as the city of Curitiba and its surroundings. The purpose of the present study was to determine the seroprevalence of visceral leishmaniasis in dogs at the Center for Zoonosis Control of São José dos Pinhais, Paraná, before euthanasia. Enzyme-linked immunosorbent assay (ELISA and immunofluorescence antibody test (IFAT were used to detect antibody levels against Leishmania sp. in dog sera. Imprints of the popliteal lymph nodes that were also randomly collected from 50 dogs with suspected clinical signs of visceral leishmaniasis, and evaluated under light microscopy for the detection of amastigote forms, were negative. A total of 364 dog samples were tested. The results showed only one positive sample (0.0027% by ELISA test but negative by IFAT, however, the dog had no clinical signs. Random surveillance of dog populations from several districts of a metropolitan area may be a means of preventing Leishmania spreading. Based on our results, the city of Curitiba and its metropolitan area were considered at low risk for visceral leishmaniasis.A leishmaniose é uma importante parasitose re-emergente observada no mundo, particularmente em países tropicais. Não há ainda relatos de casos autóctones no estado do Paraná. Não há até o momento referência de vigilância no reservatório canino, tais como Curitiba e região metropolitana do estado. O objetivo do estudo foi determinar a soroprevalência da leishmaniose visceral em cães entregues ao Centro de Controle de Zoonoses de São José dos Pinhais, Paraná para eutanásia. A detecção sorológica da presença de anticorpos contra Leishmania sp. foi realizada por (ELISA indireto e pela Reação de Imunofluorescência Indireta (RIFI. Al

  18. Hepatic granulomas in canine visceral leishmaniasis and clinical status Granulomas hepáticos na leishmaniose visceral canina e classificação clínica

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    J.A.P. Sant’Ana

    2007-10-01

    Full Text Available The histopathological description of intralobular hepatic granulomas in animals with a defined clinical status (asymptomatic, oligosymptomatic and symptomatic animals was reported. Seventy-one mongrel dogs naturally infected with Leishmania chagasi were obtained from two Brazilian endemic areas: João Pessoa, PB and Belo Horizonte, MG. The hepatic parasite load was determined and compared to granuloma formation. Liver fragments from all infected animals showed remarkable leishmaniotic granulomatous inflammatory reaction. Granulomas with variable size were constituted by macrophages (parasitized or not with amastigotes of L. chagasi, some epithelioid cells, small numbers of lymphocytes, plasma cells, and rare neutrophils. Asymptomatic dogs had higher numbers of granulomas than oligosymptomatic and symptomatic animals from both geographical regions. However, the average diametric size of granulomas was very heterogeneous in all groups, independently of the geographic region (P>0.05. Parasite tissue load did not show any difference among liver fragments of all animals, especially when considering the defined clinical status and/or their geographic origin.Descreve-se a formação de granulomas hepáticos na leishmaniose canina em animais com classificação clínica definida - assintomáticos, oligossintomáticos e sintomáticos. Setenta e um animais, sem raça definida e naturalmente infectados com Leishmania chagasi, foram obtidos de duas regiões endêmicas brasileiras: João Pessoa, PB e Belo Horizonte, MG. A carga parasitária tecidual foi determinada mediante emprego do Leishmania Donovani Units (LDU e comparada com a formação de granulomas hepáticos. Fragmentos de fígado de todos os animais infectados mostraram reação granulomatosa notadamente leishmaniótica. Granulomas de variáveis tamanhos eram constituídos por macrófagos, parasitados ou não com formas amastigotas de L. chagasi, algumas células epitelióides, pequeno número de

  19. Aspectos epidemiológicos da Leishmaniose Visceral humana e canina em municípios pertencentes à Superintendência Regional de Saúde de Diamantina, Minas Gerais, Brasil (2007-2012

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    Renata Luiz URSINE

    2016-05-01

    Full Text Available O presente estudo teve como objetivo identificar as áreas de ocorrência e avaliar aspectos epidemiológicos da Leishmaniose Visceral humana e canina nos 33 municípios pertencentes à Superintendência Regional de Saúde de Diamantina no período de 2007 a 2012. Foram coletados dados de notificação dos casos de Leishmaniose Visceral humana, disponibilizados no Sistema de Informação de Agravos e de Notificação, e dados de exames sorológicos provenientes de inquéritos caninos registrados em livros da Superintendência Regional de Saúde de Diamantina. No período avaliado foram notificados 79 casos da Leishmaniose Visceral humana, sendo a ocorrência mais comum entre pessoas com idade inferior a 10 anos e igual ou acima de 60 anos, do sexo masculino, residentes em áreas rurais. Araçuaí foi o município que apresentou maior número de notificações da Leishmaniose Visceral humana, sendo classificado como área de transmissão intensa. Quanto à Leishmaniose Visceral canina, 17 municípios enviaram amostras de sangue para realização dos exames e todos apresentaram pelo menos um cão soropositivo. Foi verificado que alguns municípios notificaram a Leishmaniose Visceral em humanos e em cães, outros, notificaram a infecção apenas em cães ou em pessoas e ainda, municípios que não apresentaram nenhuma notificação no período avaliado. Entre os municípios estudados foi possível observar a condução diferenciada da vigilância da Leishmaniose Visceral. Sendo atualmente responsabilidade direta da esfera municipal, cabe ao nível regional orientar e fiscalizar os gestores nas ações de controle de acordo com a situação epidemiológica.

  20. Canine visceral leishmaniasis: seroprevalence and risk factors in Cuiabá, Mato Grosso, Brazil Leishmaniose visceral canina: soroprevalência e fatores de risco em Cuiabá, Mato Grosso, Brasil

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    Arleana do Bom Parto Ferreira de Almeida

    2012-12-01

    Full Text Available In Brazil, canine visceral leishmaniasis (CVL is endemic and the number of cases in humans and dogs has increased in the Midwest region. A transversal study was carried out in endemic areas from Cuiabá, State of Mato Grosso, to assess data on seroprevalence and risk factors associated to canine infection. Four hundred and thirty (430 dogs were randomly evaluated through indirect fluorescence antibody test (IFAT considering variables related to the animals, the environment and the knowledge by owners on CVL aspects and control. From 430 dogs, 95 (22.1% were seroreagent for leishmaniasis and animals living in rural environments present risk 1.9 times higher for acquiring the disease than those in urban environments (p = 0.01; OR 1.9. Factors related to animals' habits, such as free access to the street and guard function were considered indicators to predict infection by Leishmania sp. (p No Brasil, a leishmaniose visceral canina (CLV é endêmica e, na região Centro-Oeste, o número de casos em humanos e cães tem aumentado. Um estudo transversal foi realizado em áreas endêmicas de Cuiabá (MT com objetivo de avaliar dados sobre a soroprevalência e determinar os fatores de risco associados à infecção canina. Quatrocentos e trinta (430 cães foram aleatoriamente avaliados pelo teste de imunofluorescência indireta, considerando-se variáveis relacionadas aos animais, o ambiente e o conhecimento por parte dos proprietários sobre aspectos da CLV e seu controle. Dos 430 cães, 95 (22,1% apresentaram-se soros reagentes para leishmaniose, e os animais que viviam em ambiente rural apresentaram risco 1,9 vezes maior de adquirir a infecção dos que aqueles em ambiente urbano (p = 0,01; OR 1,9. Fatores relacionados aos hábitos dos animais, tais como o livre acesso à rua e função de guarda, foram considerados indicadores para prever a infecção por Leishmania sp. (p.< 0,05 em análise estatística univariada. A presença de atividade agr

  1. Análise TG-ROC de testes de imunofluorescência no diagnóstico de leishmaniose visceral canina Análisis TG-ROC de pruebas de inmunofluorescencia en el diagnóstico de leishmaniasis visceral canina TG-ROC analysis of immunofluorescence assays in canine visceral leishmaniasis diagnosis

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    Rita Maria da Silva

    2009-12-01

    Full Text Available OBJETIVO: Analisar a acurácia do diagnóstico de dois protocolos de imunofluorescência indireta para leishmaniose visceral canina. MÉTODOS: Cães provenientes de inquérito soroepidemiológico realizado em área endêmica nos municípios de Araçatuba e de Andradina, na região noroeste do estado de São Paulo, em 2003, e área não endêmica da região metropolitana de São Paulo, foram utilizados para avaliar comparativamente dois protocolos da reação de imunofluorescência indireta (RIFI para leishmaniose: um utilizando antígeno heterólogo Leishmania major (RIFI-BM e outro utilizando antígeno homólogo Leishmania chagasi (RIFI-CH. Para estimar acurácia utilizou-se a análise two-graph receiver operating characteristic (TG-ROC. A análise TG-ROC comparou as leituras da diluição 1:20 do antígeno homólogo (RIFI-CH, consideradas como teste referência, com as diluições da RIFI-BM (antígeno heterólogo. RESULTADOS: A diluição 1:20 do teste RIFI-CH apresentou o melhor coeficiente de contingência (0,755 e a maior força de associação entre as duas variáveis estudadas (qui-quadrado=124,3, sendo considerada a diluição-referência do teste nas comparações com as diferentes diluições do teste RIFI-BM. Os melhores resultados do RIFI-BM foram obtidos na diluição 1:40, com melhor coeficiente de contingência (0,680 e maior força de associação (qui-quadrado=80,8. Com a mudança do ponto de corte sugerido nesta análise para a diluição 1:40 da RIFI-BM, o valor do parâmetro especificidade aumentou de 57,5% para 97,7%, embora a diluição 1:80 tivesse apresentado a melhor estimativa para sensibilidade (80,2% com o novo ponto de corte. CONCLUSÕES: A análise TG-ROC pode fornecer importantes informações sobre os testes de diagnósticos, além de apresentar sugestões sobre pontos de cortes que podem melhorar as estimativas de sensibilidade e especificidade do teste, e avaliá-los a luz do melhor custo

  2. Aspectos epidemiológicos da leishmaniose visceral canina no município de Pedro Leopoldo, Minas Gerais, 2003 Epidemiologic aspects of canine visceral leishmaniasis in Pedro Leopoldo district, Minas Gerais, 2003

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    L.A.B. Naveda

    2006-12-01

    Full Text Available Determinou-se a prevalência da leishmaniose visceral canina (LVC no município de Pedro Leopoldo, MG, 2003, por meio de inquérito soro-epidemiológico. Coletaram-se amostras de sangue de 2.185 cães e aplicou-se questionário semi-estruturado em cada residência. Os animais foram divididos em quatro grupos, sendo o grupo A constituído de 1240 cães das áreas urbana e rural; o grupo B de 705 cães de áreas em torno dos focos; o grupo C, de 134 cães, cujas amostras foram coletadas durante o dia da vacinação anti-rábica oficial; e o grupo D composto de 111 cães errantes. Os dados foram armazenados em banco de dados EPIINFO 6.04b. Os 36 cães positivos foram localizados espacialmente com auxílio de GPS (Global Positioning System, e o georreferenciamento realizado por meio do programa AUTOCAD 2000. A prevalência global da LVC foi de 1,4% e variou de 1,1% na área urbana e 4,2% na rural. Estes resultados permitiram concluir que Pedro Leopoldo, de acordo com a classificação oficial do Ministério da Saúde, é área silenciosa, receptiva e vulnerável para LVC. A distribuição espacial dos casos é diferenciada e requer medidas sanitárias de acordo com cada área de risco.The prevalence of canine visceral leishmaniasis (CVL in Pedro Leopoldo district, 2003 was evaluated. A serum-epidemic inquiry was accomplished by collecting blood samples of dogs and by applying a semi structured questionnaire, according to groups, in each visited residence: group A, constituted of 1240 dogs of the urban and rural areas; group B, constituted of 705 dogs sampled around the focuses; group C, constituted of 134 dogs, wich samples were collected during the canine/feline antirabic vaccination; and group D, constituted of 111 wandering dogs. The data were stored in the EPI INFO 6.04b database. Positive dogs were spatially located using GPS (Global Positioning System and the geographycal reference was made through the AUTOCAD 2000 program. The district global

  3. Evaluación de una prueba de aglutinación directa como método alternativo para el diagnóstico de leishmaniasis visceral canina y humana en Venezuela

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    Guillermo Terán-Angel

    2007-09-01

    Resultados. Todas las muestras de los pacientes con leishmaniasis visceral activa o curada fueron positivas. Mostraron títulos anti-L. donovani por encima de 1:1.600. Tres de cuatro casos con sospecha de leishmaniasis visceral también resultaron positivos a la prueba, mientras que los sueros controles y los de los pacientes con otras patologías dieron títulos por debajo de 1:800. Se obtuvo una sensibilidad de 100% a todos los puntos de corte considerados y una especificidad de 100% al punto de corte de 1:800 (intervalo de confianza de 95%, IC95%: 90,97%-100%. Las muestras de perros mostraron una sensibilidad de 92,59% (IC95%: 75,69%-99,09% y 100% de especificidad (IC95%: 79,42%-100% al punto de corte de 1:200. Conclusión. En general, nuestros resultados indican que el uso de la prueba de aglutinación directa es apropiado para la realización de estudios epidemiológicos y para el diagnóstico serológico de leishmaniasis visceral humana y canina en las áreas rurales de Venezuela.

  4. Autochthonous canine visceral leishmaniasis in a non-endemic area: Bom Sucesso, Minas Gerais State, Brazil Ocorrência de leishmaniose visceral canina autóctone em uma área não-endêmica: Bom Sucesso, Minas Gerais, Brasil

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    Marcio Roberto Silva

    2008-02-01

    sintomas característicos de leishmaniose visceral canina. Os resultados desta investigação epidemiológica foram enviados às autoridades locais e estaduais de saúde pública requerendo medidas preventivas e de controle para leishmaniose visceral de forma a interromper a transmissão da doença e evitar a ocorrência de casos humanos.

  5. Fauna de flebotomíneos (Diptera: Psychodidae em área endêmica de leishmaniose visceral canina na região metropolitana de São Luís – MA, Brasil

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    Tatiane Aranha da Penha

    2013-01-01

    Este estudo objetivou identificar a fauna de Phlebotominae (Diptera Psychodidae em área com ocorrência de leishmaniose visceral do município de São Luís, MA. A captura dos flebotomíneos foi realizada no distrito do Tirirical, com o uso de armadilhas luminosas do tipo CDC no intradomicílio e peridomicílio (abrigo de animais das 18 às 6 horas, uma vez por mês, em quatro pontos fixos de coleta, de agosto de 2005 a julho de 2006. Um total de 4.326 flebotomíneos (machos: 2.808/64,9% e fêmeas: 1.518/35,1% foi capturado. Sete espécies foram identificadas: Lutzomyia longipalpis (Lutz & Neiva (2.317/53,5%, L. whitmani (Antunes & Coutinho (1.761/40,7%, L. antunesi (Coutinho (120/2,8%, L. evandroi (Costa Lima & Antunes (99/2,3%, L. sordellii (Shannon & Del Ponte (14/0,3%, L. flaviscutellata (Mangabeira (12/0,3% e L. richardwardi (Ready & Fraiha (3/0,1%. Lutzomyia longipalpis e L. whitmani, as espécies mais prevalentes, ocorreram durante todo o ano. A presença de L. longipalpis explica a transmissão de casos autóctones de leishmaniose visceral canina. Destaca-se, ainda, a presença de L. whitmani e L. flaviscutellata, vetores de Leishmania braziliensis and L. amazonensis, agentes etiológicos de leishmaniose cutânea e/ou mucocutânea e leishmaniose cutânea difusa, respectivamente.

  6. Phlebotomine sand flies and canine infection in areas of human visceral leishmaniasis, Cuiabá, Mato Grosso Flebotomíneos e infecção canina em áreas de leishmaniose visceral humana, Cuiabá, Mato Grosso

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    Gustavo Leandro da Cruz Mestre

    2011-09-01

    Full Text Available Visceral leishmaniasis is a systemic infectious disease that can cause to a severe, potentially life-threatening chronic condition in humans. Risk factors for infection in urban areas have been associated with poor living conditions, the presence of sand fly vectors and infected pets. This study aimed to describe sand fly and canine infection in the neighborhoods of human visceral leishmaniasis occurrence in the city of Cuiabá, Mato Grosso State, central-western Brazil, reported between January 2005 and December 2006. A total of 1,909 sand flies were collected. They were predominantly males and the most frequent species were Lutzomyia cruzi (81.25%, Lutzomyia whitmani (13.88% and Lutzomyia longipalpis (2.62%. The sand fly density was not significantly correlated with the variation of environmental factors. The prevalence of canine visceral leishmaniasis in the neighborhoods studied was 26.82% and it was found that areas with high density of vectors coincided with areas of high prevalence of dogs and those with the highest rates of human cases. The study of vectors and other potential hosts are essential for a good understanding of visceral leishmaniasis and the related public health concerns, aiming at the prevention and control of leishmaniasis in the city of Cuiabá, Mato Grosso State.A leishmaniose visceral é uma doença infecciosa sistêmica, de evolução crônica grave, potencialmente fatal para o homem. Os fatores de risco para a infecção em áreas urbanas têm sido associados às precárias condições de moradia, à presença de flebotomíneos vetores e de animais domésticos infectados. O presente trabalho objetivou descrever a fauna flebotomínica e infecção canina nos bairros de ocorrência da leishmaniose visceral humana no município de Cuiabá, Mato Grosso, notificados no período de janeiro de 2005 a dezembro de 2006. Foram coletados 1.909 flebotomíneos, as espécies mais frequentes foram Lutzomyia cruzi (81,25%, Lutzomyia

  7. Canine visceral leishmaniasis in the Krenak indigenous community, Resplendor, Minas Gerais State, Brazil, 2007 Leishmaniose visceral canina na Terra Indígena Krenak, Resplendor, Minas Gerais, Brasil, 2007

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    Eloiza Gonçalves Antônio

    2011-03-01

    Full Text Available The authors conducted a cross-sectional study of the local canine population in the Krenak indigenous community to detect parasites of the genus Leishmania and identify the circulating species and the proportion of asymptomatic dogs, while investigating associations between canine infection and the dogs' sex, age, and hair length. A seroepidemiological survey was performed, including 63 dogs. All the animals underwent clinical examination to verify the presence of characteristic signs, and serum samples were taken for serological tests (ELISA, IIF. Infected dogs culled by the health service were necropsied and the material was analyzed using molecular diagnostic techniques. The cross-sectional study detected a 46% prevalence rate, and the circulating species was Leishmania (L. chagasi. The statistical analysis showed no association between infection and the independent variables. The study generated data on the epidemiological situation with canine infection in the area, which was previously unknown.Foi realizado um estudo seccional para detectar, na população canina, a presença de parasitos do gênero Leishmania e a espécie circulante, a proporção de cães assintomáticos, investigando concomitantemente a existência de associações entre a infecção canina e as variáveis: sexo, idade e tipo de pelo dos cães. Para o estudo seccional, foi realizado um inquérito censitário, que avaliou 63 cães. Todos passaram por uma avaliação clínica para verificar a presença de sintomas característicos da infecção; amostras de soro foram coletadas para os testes sorológicos (ELISA, RIFI. Os cães positivos retirados pelo serviço de saúde foram necropsiados, e o material, analisado pelas técnicas de diagnóstico molecular. O estudo seccional realizado detectou uma prevalência de 46%, sendo a espécie circulante a Leishmania (L. chagasi. A análise estatística não detectou nenhuma associação entre infecção e as vari

  8. Leishmaniose visceral canina: avaliação da metodologia sorológica utilizada em inquéritos epidemiológicos

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    Carlos A. da Costa

    1991-03-01

    Full Text Available Foi realizado um estudo comparativo da reação de imunofluorescência indireta em eluatos de sangue de cães infectados experimentalmente com diferentes tripanosomatídeos. Utilizaram-se como antigenopromastigotas de L. mexicana, L. braziliensis e L. chagasi. Os resultados mostraram que a sensibilidade do método foi de 87,5% para o diagnóstico do calazar canino, independentemente do antigeno empregado; e que ocorre reação cruzada com Leishmaniose tegumentar em 75% dos casos e com doença de Chagas em 83,3%. Levantamento epidemiológico em área de leishmaniose confirma que a reação de imunofluorescência em eluatos de sangue canino fornece reações cruzadas em cães infectados com Leishmania braziliensis e L. chagasi. Não se verificou reação cruzada pela RFC. Sugere-se a utilização da reação de imunofluorescência nas campanhas de saúde pública, mas é de se chamar a atenção para o fato de que as taxas de positividade não devem ser utilizadas como indicadores da prevalência do calazar canino.A comparative study was made of eluates of the blood of dogs experimentally infected with different trypanosomatids. Using antigens prepared from promastigotes of Leishmania mexicana, L. braziliensis and L. chagasi, assessments were made by the indirect immunofluorescence test. The results showed a sensitivity of 87,5% in the diagnosis of canine visceral leishmaniasis, independent of antigen used. Cross-reactions occurred in 75% of cases of cutaneous leishmaniasis and 83,3% of dogs with chagas' disease. An epidemiological survey in an area of leishmaniasis confirmed that immunofluorescence tests on eluates of dogs' blood give cross-reactions between L. braziliensis and L. chagasi. The results suggest that such testing could be useful in public health campaigns but attention is drawn to thefact that the level ofpositive reactions cannot be used as an indicator of the prevalence of canine kala-azar.

  9. Flebotomíneos de áreas com notificações de casos autóctones de leishmaniose visceral canina e leishmaniose tegumentar americana em Angra dos Reis, Rio de Janeiro, Brasil Phlebotomine fauna in areas of autochthonous cases of canine visceral leishmaniasis and American cutaneous leishmaniasis in Angra dos Reis, Rio de Janeiro, Brazil

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    Marcos Barbosa de Souza

    2009-01-01

    Full Text Available Flebótomos de áreas com notificações de casos autóctones de leishmaniose visceral canina e leishmaniose tegumentar americana em Angra dos Reis, Rio de Janeiro, Brasil. O município de Angra dos Reis apresenta casos humanos de leishmaniose tegumentar americana desde 1945. Inquéritos flebotomínicos realizados em 1978 revelaram a presença de Nyssomyia intermedia e a primeira notificação de Lutzomyia longipalpis no Rio de Janeiro, Ilha Grande, Angra dos Reis. Em agosto de 2002 foi notificado o primeiro caso canino de leishmaniose visceral na Ilha Grande, Angra dos Reis. Inquéritos flebotomínicos realizados nos peridomicílios, no período de novembro de 2002 a maio de 2003, em quatro localidades de Angra dos Reis, resultaram em 12.554 flebotomíneos e a presença de nove espécies: Brumptomyia sp.; Nyssomyia intermedia, Migonemyia migonei, Micropygomyia schreiberi, Pintomyia fischeri, Psychodopygus davisi, Psychodopygus ayrosai, Evandromyia tupinambay, Psathyromyia pelloni. foi Nyssomyia intermedia, predominante em todas as localidades, seguida por M.migonei. O principal vetor da LVA, Lutzomyia longipalpis, não foi detectado nas localidades, incluindo áreas do entorno, onde um cão infectado residia.The city of Angra dos Reis presents human cases of American cutaneous leishmaniasis since 1945. Phlebotomine surveys in 1978 had disclosed the presence of Nyssomyia intermedia and the first notification of Lutzomyia longipalpis in Rio de Janeiro, Ilha Grande, Angra dos Reis. In August of 2002 the first canine case of visceral leishmaniasis in the Ilha Grande, Angra dos Reis was notified. Surveys of phlebotomines in the peridomiciliary areas, in the period of November, 2002, through May, 2003, in four localities of Angra dos Reis, resulted in 12,554 specimens belonging to nine species: Brumptomyia sp. Nyssomyia intermedia, Migonemyia migonei, Micropygomyia schreiberi, Pintomyia fischeri, Psychodopygus davisi, Psychodopygus ayrosai

  10. Aspectos epidemiológicos da leishmaniose visceral canina na zona rural do semiárido paraibano e análise de técnicas de diagnóstico

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    Raizza B.S. Silva

    Full Text Available Resumo: No Brasil, a leishmaniose visceral é causada pela Leishmania infantum. É uma zoonose crônica e, frequentemente, fatal, sendo considerada um grave problema de saúde pública no mundo e reconhecida pela Organização Mundial de Saúde como uma das dezessete "Doenças Tropicais Negligenciadas". O presente estudo foi realizado em 2012, na zona rural do município de Patos, semiárido paraibano, com o objetivo de estimar a prevalência, os fatores de risco da leishmaniose visceral canina (LVC na região e avaliar os testes de diagnóstico sorológico. Foram coletadas 362 amostras de sangue e a prevalência da infecção foi determinada através de três técnicas sorológicas (ELISA, RIFI e Teste Imunocromatográfico DPP - Dual Path Plataform, sendo consideradas positivas as amostras que apresentaram pelo menos dois testes reagentes. As amostras positivas na sorologia foram testadas na PCR e qPCR. Aplicou-se um questionário epidemiológico aos proprietários para identificação dos possíveis fatores de risco. A taxa de prevalência foi de 11,33% (41/362. Considerou-se o ELISA como padrão ouro, já que é o teste confirmatório para a LVC pelo Ministério da Saúde. O DPP (Dual Path Plataform apresentou sensibilidade de 58% (95% CI: 0,43-0,72 e especificidade de 96% (95% CI: 0,93-0,98, com índice Kappa de 0,57. A RIFI apresentou sensibilidade de 85% (95% CI: 0,72-0,94, especificidade de 99% (95% CI: 0,98-1 e valor Kappa de 0,88. O fator de risco identificado na análise multivariada por regressão logística múltipla foi o sexo (OR=2,15 [1,09-4,21] e os cães machos apresentaram 2,15 vezes mais chances de serem infectados, que pode ser justificado por serem mais utilizados para a caça e como cão de guarda, dois fatores que aumentam a exposição do animal ao flebótomo. Em função da taxa de prevalência encontrada, indica-se a aplicação de medidas de controle no município. Os resultados da comparação dos ensaios sorol

  11. Canine visceral leishmaniasis epidemiological survey in three sanitary districts of Salvador, Bahia state, Brazil Inquérito epidemiológico da leishmaniose visceral canina em três distritos sanitários do Município de Salvador, Bahia, Brasil

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    Fred da Silva Julião

    2009-06-01

    Full Text Available The goal of the present work was to investigate the occurence of canine Visceral Leishmaniasis (VL in Itapuã, Cajazeiras and Pau da Lima sanitary districts (SD, all belonging to the city of Salvador. In order to achieve it, a canine epidemiological and entomological surveys were applied as an epidemiological questionnaire to the owners of the animals sampled and to the veterinary physicians of the city. The study detected six VL seropositive dogs among the eight hundred and eleven ones randomly sampled. It was not possible to determine the autoctonous condition for L. chagasi infection. One animal was positive by culture of splenic material and in the entomologic survey of the vector, Lutzomyia longipalpis, none was captured. Veterinary clinicians from 41.7% of the clinics evaluated reported the diagnostic of, at least, one VL case in Salvador domiciliated dogs. The SD of Itapuã, Cajazeiras and Pau da Lima presented VL transmission risk once there is Leishmania parasite occurrence in the municipality, verified in this study by splenic culturing of an animal aspirative puncture, previous record of vector (L. longipalpis presence in the Itapuã district by the Bahia State Health Agency (SESAB and canine VL diagnostics by veterinary doctors from clinics all around the city of Salvador. This work is the first epidemiological analysis of this zoonosis in these SD and they point out the need to perform sampled entomological and serological surveys in all areas of the city. The development of a VL surveillance system may contribute for monitoring the disease in Salvador.Objetivou-se com este estudo investigar a ocorrência da Leishmaniose Visceral (LV canina nos Distritos Sanitários (DS de Itapuã, Cajazeiras e Pau da Lima, pertencentes ao município de Salvador, por meio da realização de inquérito sorológico canino; inquérito entomológico, aplicação de questionário epidemiológico aos proprietários dos animais amostrados e a m

  12. Allochthonous case of visceral canine leishmaniasis in Campo Mourão, Parana, BrazilCaso alóctone de leishmaniose visceral canina, no município de Campo Mourão, Paraná, Brasil

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    Sabrina Marin Rodigheri

    2012-05-01

    Full Text Available This report aims to describe the first case allochthonous of visceral leishmanisasis (VL in the municipality of Campo Mourão, Parana, Brazil, in a canine, Boxer breed, with eye and skin lesions, lymphadenomegaly and splenomegaly, attended at Veterinary Hospital at the Faculdade Integrado de Campo Mourão, after residing in the city of Campo Grande, Mato Grosso do Sul. The diagnosis of the disease was based on direct observation of amastigotes of Leishmania spp., in popliteal lymph nodes, suggesting that a case of VL, since the animal came from an endemic area for the disease. The migration of infected dogs from endemic regions to areas unaffected becomes a problem for public health, since it may allow the installation of new outbreaks by encouraging the spread of disease throughout the country.O presente relato tem por objetivo descrever o primeiro caso alóctone de leishmaniose visceral (LV no município de Campo Mourão, Paraná, Brasil, em um canino, da raça Boxer, apresentando lesões oculares e cutâneas, linfoadenomegalia e esplenomegalia, atendido no Hospital Veterinário da Faculdade Integrado de Campo Mourão, após ter residido na cidade de Campo Grande, Mato Grosso do Sul. O diagnóstico da enfermidade baseou-se na observação direta de formas amastigotas de Leishmania spp., em linfonodos poplíteos, sugerindo ser um caso de LV, uma vez que o animal era proveniente de área endêmica para a enfermidade. A migração de cães infectados de regiões endêmicas para áreas indenes torna-se um problema para a saúde pública, uma vez que poderá permitir a instalação de novos focos, favorecendo a disseminação da doença em todo o país.

  13. Canine visceral leishmaniasis: a remarkable histopathological picture of one asymptomatic animal reported from Belo Horizonte, Minas Gerais, Brazil Leishmaniose visceral canina: um caso inusitado de um animal assintomático proveniente de Belo Horizonte, Minas Gerais

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    S.C. Xavier

    2006-12-01

    Full Text Available A remarkable histopathological picture of one asymptomatic dog naturally infected with Leishmania infantum (syn. chagasi has been presented. Intracellular parasites were ease found in macrophages of all exanimated organs, especially in skin. Embedded paraffin tissues of liver, spleen, axillary and popliteal lymph nodes, and skin (ear, muzzle and abdomen were stained by hematoxylin and eosin and by immunocytochemical reaction (streptoavidin-peroxidase method to detect parasites. All organs showed an intense parasitism associated to severe pathological changes. All lymph nodes had conspicuous histological architecture alterations. Lymphocytes were replaced by macrophages stuffed with an intense number of amastigotes forms of Leishmania. The lymphoid nodules (without germinal centers and the mantle zones in the cortex that surround the follicles were markedly attenuated. Livers showed small intralobular granulomas composed by macrophages loaded with amastigotes. Spleens had an intense depression of the white pulp whereas the lymphocytes were replaced by parasitized macrophages. All fragments of different anatomical region of skin (ear, muzzle and abdomen showed a diffuse chronic inflammation. The cellular exudate was composed by macrophages, plasmocytes and lymphocytes. Macrophages loaded with amastigotes were ease found in all tissue fragments, but more intense in ear and muzzle. Thus, this fact enhances the importance of asymptomatic dogs in the epidemiology of visceral leishmaniasis.Relata-se um quadro histológico caracterizado por lesões acentuadas em tecidos de um cão assintomático naturalmente infectado por Leishmania infantum (sin. chagasi. Cortes parafinados de fígado, baço, linfonodos (cervical, axilar e poplíteo e pele (orelha, espelho nasal e abdome foram corados pela técnica de hematoxilina-eosina e pela técnica imunoistoquímica de estreptoavidina-peroxidase para detecção de formas amastigotas de Leishmania. Os linfonodos

  14. Canine visceral leishmaniasis: study of methods for the detection of IgG in serum and eluate samples Leishmaniose visceral canina: avaliação de métodos para detecção de IgG em amostras de soro e eluato

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    Fabiano B. Figueiredo

    2010-08-01

    Full Text Available The Brazilian Ministry of Health recommends the culling and euthanasia of dogs with a positive serological test for canine visceral leishmaniasis (CVL. In the Municipality of Rio de Janeiro, the technique used for the diagnosis of CVL is the indirect fluorescent antibody test (IFAT, using blood samples eluted on filter paper (eluate. A dog survey was conducted over a period of one year in the region of Carapiá, in order to evaluate the diagnosis of CVL in this region. All animals underwent clinical examination, and blood samples (serum and eluate were collected for analysis by enzyme immunoassay (ELISA and IFAT. A skin biopsy was obtained for parasitological examination (culture. A total of 305 animals were studied and Leishmania chagasi was isolated from nine animals. Sensitivity and specificity were 100% and 96.6% for ELISA, respectively, 100% and 65.5% for IFAT (cut-off at a 1:40 dilution, 100% and 83.4% for IFAT (cut-off at a 1:80 dilution, and 22.2% and 97.0% for eluate IFAT. In conclusion, ELISA was the best tool for the diagnosis of CVL among the serological techniques tested. The present results suggest the need for a better evaluation of filter paper IFAT as the only diagnostic method for CVL in the Municipality of Rio de Janeiro.O Ministério da Saúde recomenda a eutanásia de cães sororreatores como controle da leishmaniose visceral canina (LVC. No Município do Rio de Janeiro, a técnica utilizada para o diagnóstico da LVC é o teste de imunofluorescência indireta (IFI, utilizando amostras de sangue eluídas em papel de filtro (eluato. Um levantamento, durante um ano, foi conduzido na região de Carapiá, a fim de avaliar o diagnóstico da LVC nesta região. Todos os animais foram submetidos a exame clínico e coleta de sangue (soro e eluato para realização do ensaio imunoenzimático (ELISA e imunofluorescência indireta (IFI. Biópsia de pele foi obtida para o exame parasitológico (cultura. Foram avaliados 305 (89,4% animais

  15. Diagnóstico da Leishmaniose Visceral Canina pelas técnicas de imunoistoquímica e PCR em tecidos cutâneos em associação com a RIFI e ELISA-teste Canine Visceral Leishmaniasis diagnosis by immunohistochemistry and PCR in skin tissues in association with RIFI and ELISA-test

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    Nina M. G. P. de Queiroz

    2010-03-01

    Full Text Available O objetivo deste trabalho foi avaliar as técnicas de imunoistoquímica (IMIQ e de PCR (Reação em Cadeia da Polimerase em tecidos cutâneos para o diagnóstico da Leishmaniose Visceral Canina (LVC e compará-los com os exames parasitológicos em tecidos corados histoquimicamente (hematoxilina-eosina, HE e com testes sorológicos, como a Reação de Imunofluorescência Indireta (RIFI e ensaio imunoenzimático (ELISA. Dos 34 cães naturalmente infectados, classificados em assintomáticos, oligossintomáticos e polissintomáticos, foram coletadas amostras de pele sadia ou com lesão para a realização da IMIQ, HE e PCR. Não somente peles lesionadas (56,5%, mas também sadias (31,8% encontravam-se positivas pela IMIQ, confirmadas posteriormente pela PCR em 97,8% das amostras. No grupo assintomático, 87,5% estavam negativos pelos testes sorológicos, mas positivos em 50% dos casos pela IMIQ e 100% pela PCR. Entre os oligossintomáticos, 100%, 85,7% e 28,6% encontravam-se positivos, respectivamente, pela PCR, sorologia e IMIQ. Os cães polissintomáticos eram 91,7% soropositivos e tinham parasitas na pele. Em geral, a técnica PCR teve maior positividade (100%. A eficiência dos testes variou de acordo com a evolução da doença, demonstrando a necessidade da associação de técnicas, usando-se IMIQ para confirmação da sorologia e a PCR apenas nos casos suspeitos após a IMIQ. Dessa forma, pode-se aumentar os níveis de positividade e contribuir para o controle desta zoonose.The purpose of the present study was to evaluate the immunohistochemistry (IMHC and PCR (Polymerase Chain Reaction tests for Canine Visceral Leishmaniasis (CVL diagnosis and compare the results with serological tests such as the indirect fluorescence antibody test (IFAT, ELISA and a parasitological test (microscopic direct examination of the parasite stained with haematoxylin and eosin - HE. For this study, samples of healthy or lesion skin tissues were obtained from 34

  16. Desempenho do teste rápido imunocromatográfico (TRI para o diagnóstico da leishmaniose visceral canina: comparação com outros métodos sorológicos em cães suspeitos de Cuiabá, Mato Grosso, Brasil

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    Bianca De Santis

    2013-06-01

    Full Text Available O teste rápido imunocromatográfico (TRI para leishmaniose visceral canina (LVC foi testado em cães suspeitos da área urbana de Cuiabá. O desempenho do teste foi comparado com RIFI e ELISA e, como padrão ouro, o teste parasitológico direto (TPD. A amostra com 45 cães foi selecionada pelo Centro de Controle de Zoonoses, sendo 23 (51,1% machos, 34 (75,5% sem raça definida e com idade estimada inferior a três anos. De acordo com os dados clínicos e os exames laboratoriais, 10 (26,3% cães foram classificados como expostos, outros 10 (26,3% como infectados e 18 (47,4% como doentes. O TRI alcançou os melhores resultados quanto à sensibilidade, 62%, elevada especificidade, 87% e para os valores preditivos positivo e negativo: 83,30% e 81,48%, respectivamente, consolidado pelo coeficiente Kappa igual a 0,50, de concordância moderada. Os resultados confirmaram TRI como um bom preditor de doença e infecção para LVC.

  17. Reflexões sobre a qualidade do diagnóstico da leishmaniose visceral canina em inquéritos epidemiológicos: o caso da epidemia de Belo Horizonte, Minas Gerais, Brasil, 1993-1997

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    Alves Waneska Alexandre

    2004-01-01

    Full Text Available No Brasil, a leishmaniose visceral (LV apresenta quadros graves de endemias e epidemias havendo, nos últimos anos, uma propagação da doença por vários estados que antes não possuíam casos de pessoas e animais. O Ministério da Saúde recomenda, para os inquéritos caninos, o uso da reação de imunofluorescência indireta (RIFI, apresentando sensibilidade de 90-100% e especificidade de 80% para amostras de soro. A utilização da RIFI pode comprometer a efetividade do Programa de Controle do Calazar por estar deixando de detectar e sacrificar animais infectados (falsos negativos. Por outro lado, o programa estaria identificando e recomendando o sacrifício de animais não infectados (falsos positivos. Essas incertezas já geraram atitudes como a não indicação do sacrifício de animais positivos e a recomendação de tratamento desses animais por parte de clínicos veterinários. Este trabalho tem como objetivo refletir sobre a qualidade do diagnóstico de inquéritos epidemiológicos caninos, tendo como pano de fundo a epidemia de LV que ocorre no Município de Belo Horizonte, Minas Gerais, desde 1993.

  18. Avaliação da confiabilidade entre dois observadores em exames citopatológico e imunocitoquímico de aspirado de medula óssea no diagnóstico da leishmaniose visceral canina

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    T.C.B. Vasconcelos

    2016-06-01

    Full Text Available ABSTRACT Visceral leishmaniasis is a zoonosis in which the dog appears as the main source of infection in urban areas. Its diagnosis is complex and the cytopathological exam is a fast and cheap alternative to parasite direct visualization and its sensitivity can be increased by immunocytochemistry, though with a higher cost. The accuracy of such methods is dependent on the microscopist's experience and therefore, this study evaluated the reliability of such techniques between two observers, from bone marrow aspirates of 50 dogs from an endemic area for the disease. The parasitological culture in Novy-MacNeal-Nicolle medium was used as the reference standard. Among the main findings, the sensitivities obtained by observers I and II were respectively 62.5% and 37.5%, while specificities were 81.1% and 100%. On immunocytochemistry evaluation, the sensitivity was 0% for both evaluators and the specificity 97.3% and 100%. The agreement between evaluators was weak (κ = 0.167 for the cytopathological test and it could not be evaluated for immunocytochemistry, for which there was no detection by the evaluator II. The agreements among the diagnostic methods and the standard reference for the observer I were reasonable (κ = 0.364 for cytopathological examination and bad (κ = -0.041 for immunocytochemistry. For observer II, such agreement could be assessed only for the cytopathological test, being moderate (κ = 0.497. The results point to the possible expertise difference between evaluators, with the evaluator II demonstrating greater experience when interpreting the citopathological test. Although there was the expected sensitivity increase with immunocytochemistry, the technique used in this study was not effective for the diagnosis of infection, regardless of the evaluator.

  19. ANTIMICROBIAL ACTIVITY OF ROSA CANINA FLOWERS AGAINST SELECTED MICROORGANISMS

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    Katarína Rovná

    2015-02-01

    Full Text Available Rosa canina flowers were screened against various plant pathogenic microbial strains to study the antimicrobial properties of the plant. Ethanolic and methanolic extracts of flowers were screened applying agar well diffusion method against two Gram-negative bacteria including Escherichia coli CCM 3988 and Pseudomonas aeruginosa CCM 1960 and three microscopic filamentous fungi strains Aspergillus niger, Fusarium culmorum and Alternaria alternata, respectively. The best antimicrobial effect of ethanolic extract of Rosa canina flowers was found against Pseudomonas aeruginosa and the best antimicrobial effect of methanolic extract of Rosa canina flowers was found against Escherichia coli.

  20. Visceral Leishmaniasis

    Science.gov (United States)

    2011-06-01

    Autoclaved Leishmania major vaccine for prevention of visceral leishmaniasis: a randomised, double-blind, BCG -controlled trial in Sudan. Lancet...nitric oxide killing. These properties of sandfly saliva are the focus of current research on an antileishmania vaccine .11 At the site of inoculation...these campaigns, incidence has returned to high levels. No VL vaccine is currently licensed or commercially available. A variety of vaccine

  1. Nota Editorial Volume X

    OpenAIRE

    Galinha,Iolanda Costa

    2014-01-01

    Neste momento de fecho do volume X da Psique, esta nota editorial tem como primeira finalidade agradecer aos especialistas em Psicologia, envolvidos no processo de revisão, selecção, e reformulação dos artigos publicados neste volume da Psique. Referimo-nos, naturalmente, ao Conselho Editorial da Psique, que foi o responsável por nos apoiar de perto neste processo ambicioso de promover, em simultâneo, a celeridade e a qualidade dos artigos a publicar neste volume da Psique. ...

  2. Nota sobre leishmaniose canina no noroeste do Estado do Paraná, sul do Brasil

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    Maria Valdrinez Campana Lonardoni; Ueslei Teodoro; Sandra Mara Alessi Aristides Arraes; Thaís Gomes Verzignassi Silveira; Dennis Armando Bertolini; Edna A.Y. Ishikawa; Jeffrey J. Shaw

    1993-01-01

    Em área endêmica de leishmaniose tegumentar americana no Município de Jussara, Estado do Paraná, Brasil, detectaram-se três cães domésticos infectados por Leishmania (Viannia) brasiliensis.In an American cutaneous leishmaniasis endemic area in Jussara county, Paraná State, Brazil, three dogs were found to be infected by Leishmania (Viannia) braziliensis.

  3. Nota sobre leishmaniose canina no noroeste do Estado do Paraná, sul do Brasil

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    Maria Valdrinez Campana Lonardoni

    1993-10-01

    Full Text Available Em área endêmica de leishmaniose tegumentar americana no Município de Jussara, Estado do Paraná, Brasil, detectaram-se três cães domésticos infectados por Leishmania (Viannia brasiliensis.

  4. Leishmaniose Canina em Brasília, DF: Uma Revisão da Literatura.

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    Tatyere Constâncio de SOUSA

    2015-09-01

    Full Text Available A leishmaniose é uma doença infecto-parasitária que acomete seres humanos e animais, causada por protozoários do gênero Leishmania. As leishmanioses são importante problema de saúde pública em vários países e estão incluídas entre as seis endemias de maior relevância mundial. São classificadas em tegumentar (LT cutânea e mucocutânea - e visceral (LV. A transmissão ocorre através de picada de insetos flebotomíneos pertencentes aos gêneros Lutzomyia (Novo Mundo e Phlebotomus(Velho Mundo. A Leishmaniose Tegumentar Americana (LTA é uma zoonose distribuída no território brasileiro. Possui evolução crônica e acomete a pele e mucosas, isoladamente ou em associação, de seres humanos, cães, gatos e eqüinos. Nos cães, se caracteriza pela presença de lesões cutâneas ulceradas, recobertas por crostas, localizadas nas orelhas, bolsa escrotal, focinho e membros. A Leishmaniose visceral é uma zoonose que ocorre nas regiões subtropicais e tropicais, causada pela Leishmania (Leishmania chagasi, no Novo e no Velho Mundo. No Brasil, a Leishmaniose Visceral Canina (LVC coexiste com a doença humana e os cães são seu reservatório doméstico. A eutanásia do cão é criticada por isso enfrenta limitações. Apesar das ações de vigilância e controle de LV adotadas no DF, a doença permanece em áreas urbanas e rurais.

  5. Effect of ethanolic extract of Rosa canina on some serum ...

    African Journals Online (AJOL)

    The aim of the present study was to investigate the effect ethanolic extract of Rosa canina on some serum biochemical factors of diabetic male rats. Thirty two (32) male Wistar rats weighting 200 – 250 grams in 4 groups (control, diabetic control, diabetics + 250mg/kg/day extract, diabetics + 500 mg/kg/day extract) were used.

  6. Phenols, essential oils and carotenoids of Rosa canina from Tunisia ...

    African Journals Online (AJOL)

    The antioxidant activity of leaf extracts of Rosa canina from diverse localities of Tunisia were evaluated by ABTS and DPPH methods, whereas in those of essential oils and carotenoids extracts such activity was determined only by the ABTS method. Total phenols determined by the Folin method revealed that at Aindraham, ...

  7. Brazilian canine hepatozoonosis Hepatozoonose canina brasileira

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    Lucia Helena O'Dwyer

    2011-09-01

    Full Text Available The genus Hepatozoon includes hundreds of species that infect birds, reptiles, amphibians and mammals, in all continents with tropical and subtropical climates. Two species have been described in domestic dogs: H. canis, reported in Europe, Asia, Africa, South America and the United States; and H. americanum, which so far has only been diagnosed in the United States. In Brazil, the only species found infecting dogs is H. canis. The objective of this review was to detail some aspects of canine hepatozoonosis, caused by H. canis, and the main points of its biology, transmission, pathogenicity, symptoms, epidemiology and diagnostic methods, with emphasis on research developed in Brazil.O gênero Hepatozoon compreende centenas de espécies que infectam aves, répteis, anfíbios e mamíferos, em todos os continentes de clima tropical e subtropical. No cão doméstico duas espécies são descritas, H. canis e H. americanum; a primeira presente na Europa, Ásia, África, América do Sul e nos Estados Unidos da América do Norte (EUA, e a segunda, diagnosticada até o momento somente nos EUA. No Brasil, a espécie que infecta o cão foi caracterizada como H. canis. Esta revisão objetiva detalhar alguns aspectos da hepatozoose canina, causada pelo H. canis e principais pontos de biologia, transmissão, patogenia e sintomas, epidemiologia e métodos de diagnóstico, enfatizando as pesquisas desenvolvidas no Brasil.

  8. Visceral larva migrans

    Science.gov (United States)

    Parasite infection - visceral larva migrans; VLM; Toxocariasis; Ocular larva migrans; Larva migrans visceralis ... Saunders; 2016:chap 39. Nash TE. Visceral larvae migrans and other uncommon helminth infections. In: Bennett JE, ...

  9. Antioxidant activity and α-amylase inhibitory potential of Rosa canina L

    African Journals Online (AJOL)

    In this study, Rosa canina was investigated for the antioxidant and α-amylase inhibition activities. Materials and Methods: Methanolic extract of Rosa canina was investigated for its potential antioxidant activity. The extracts' total phenolic and flavonoid contents and scavenging capacity for free radicals were evaluated.

  10. [Psychophysiology of visceral pain].

    Science.gov (United States)

    Horing, B; Enck, P

    2014-06-01

    The psychophysiology of visceral pain is--different from cardiac psychophysiology--much less well investigated due to the invasiveness of its methods and problems associated with reliably and reproducibly stimulating as well as recording of the gastrointestinal tract. Despite these problems, the last 30 years have documented a number of psychophysiological phenomena such as the perception (interoception) of visceral stimuli, the effect of emotions and stress on visceral sensations, and the effect of visceral processes on cortical processing. This was mainly due to the application of neurophysiological techniques (cortical imaging and stimulation) in these investigations.

  11. A expansão da epidemia da leishmaniose visceral no Estado de Mato Grosso, 1998-2005 The spread of the visceral leishmaniasis epidemic in the State of Mato Grosso, 1998-2005

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    Gustavo Leandro da Cruz Mestre

    2007-02-01

    Full Text Available Uma epidemia de leishmaniose visceral teve início em 1998 na Região Metropolitana de Cuiabá, capital de Mato Grosso, atingindo hoje 34 (24,1% dos 141 municípios do estado. Entre janeiro de 1998 e dezembro de 2005, foram notificados 138 casos autóctones, predominando o sexo masculino (58%, crianças (51,5% de 0-9 anos e residentes (66,7% de áreas urbanas. A leishmaniose visceral canina foi identificada em 41 municípios, com soropositividade de 9% em 40.000 cães examinados. Lutzomyia longipalpis e/ou Lutzomyia cruzi foram capturadas em 14 dos 18 municípios que registraram simultaneamente leishmaniose visceral humana e canina. Os resultados indicam que a transmissão da leishmaniose visceral dissemina-se para o interior do estado, acompanhando o fluxo migratório e o processo de ocupação urbana desordenada das cidades. A presença isolada de Lutzomyia cruzi em municípios com alta incidência de casos humanos e caninos de leishmaniose visceral sugere possível participação desta espécie na cadeia de transmissão dessa parasitose em Mato Grosso.An epidemic of visceral leishmaniasis began in 1998, in the Metropolitan Region of Cuiabá, the capital of the State of Mato Grosso, Brazil. Today, it has reached 34 (24.1% of the 141 municipalities in the state. Between January 1998 and December 2005, 138 autochthonous cases were notified, mainly in males (58%, children aged 0-9 years (51.5% and inhabitants of urban areas (66.7%. Canine visceral leishmaniasis has been detected in 41 municipalities, with positive serum in 9% of the 40,000 dogs examined. Lutzomyia longipalpis and/or Lutzomyia cruzi were captured in 14 out of the 18 municipalities that simultaneously recorded both human and canine visceral leishmaniasis. These findings indicate that visceral leishmaniasis transmission has become disseminated throughout the state, following migratory flows and the process of disorderly occupation of urban areas. The presence of Lutzomyia cruzi

  12. Visceral sensitivity testing.

    Science.gov (United States)

    Andresen, Viola

    2009-01-01

    Visceral hypersensitivity is regarded as an important factor in the pathogenesis of functional gastrointestinal disorders. Assessment of visceral sensitivity has several important aims: increasing the understanding of normal and abnormal visceral sensory mechanisms and participating sensory pathways, serving as diagnostic tool to detect patients with abnormal visceral sensitivity, and evaluating therapeutic interventions directed towards modification of visceral sensitivity. Current stimulation modes in sensitivity tests include mechanical distension by barostat or tensostat, nutrient drink or water load, chemical stimulation, e.g. acid provocation or capsaicin ingestion, electrical, or thermal stimulation. Multimodal probes incorporating several stimulation modes in one device have recently been developed. Assessment of visceral sensation can be based on subjective responses of conscious perception or on objective parameters such as visceromotoric responses or central sensory processing patterns. All methods face the challenge that visceral sensitivity may be influenced by a wide spectrum of different factors, including the test techniques themselves, and improved, preferably non-invasive sensitivity tests with a greater standardisation and a broader applicability are still needed.

  13. Immunity to Visceral Leishmaniasis

    OpenAIRE

    Ali, Nahid; Mekuria, Asrat Hailu; Requena, José María; Engwerda, Christian

    2012-01-01

    Leishmaniasis is a major vector-borne parasitic disease affecting 12 million people worldwide. With a broad range of clinical manifestations, ranging from self-healing skin ulcers to disfiguring mucosal lesions to life-threatening infections of visceral organs (liver and spleen), the disease has become a serious human health issue, particularly in developing countries. Among all of its forms, visceral leishmaniasis (VL, also known as kala-azar), caused by the Leishmania donovani complex (i.e....

  14. Hepatoprotective effect of Rosa canina fruit extract against carbon tetrachloride induced hepatotoxicity in rat

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    Heibatollah Sadeghi

    2016-03-01

    Full Text Available Objective: The present study was conducted to investigate the hepatoprotective activity of hydro-ethanolic fruit extract of Rosa canina (R. canina against carbon tetrachloride (CCl4-induced hepatotoxicity in rats. Methods: Male Wistar albino rats were randomly divided into six groups of 8 animals of each, including control, toxic (CCl4, R. canina 250, 500, and 750 mg/kg + CCl4 and R. canina 750 mg/kg alone. R. canina (p.o., daily and CCl4 (1 ml/kg twice a week, 50% v/v in olive oil, i.p. were administered to animals for six weeks. Serum analysis was performed to assay the levels of aspartate aminotransferase (AST, alanine amino transaminase (ALT, alkaline phosphatase (ALP, albumin (ALB, total protein (TP and malondialdehyde (MDA. Biochemical observations were also supplemented with histopathological examination (haematoxylin and eosin staining of liver section.Results: Hepatotoxicity was evidenced by considerable increase in serum levels of AST, ALT, ALP, and lipid peroxidation (MDA and decrease in levels of ALB and TP. Injection of CCL4 also induced congestion in central vein, and lymphocyte infiltration. Treatment with hydro-alcoholic fruit extract of R. canina at doses of 500 and 750 mg/kg significantly reduced CCl4-elevated levels of ALT, AST, ALP and MDA (p

  15. CHARACTERIZATION OF ENDOPHYTIC MICROFLORA OF ROSA CANINA FRUITS

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    Katarína Rovná

    2015-02-01

    Full Text Available There aren’t a lot of studies about the bacterial communities associated with the Rosa canina and the aim of this study was to characterize endophytic bacteria from fruit of Rosa canina. The fruits of R. canina, which is growing wild in Slovakia, were collected in May 2013 from four locations: Nitra-Zobor, Vrbové-Baraní dvor, Rišňovce, Modra pažiť, Slovakia. Microbiological analyses were conducted by use of standard microbiological methods by spreading of fruits homogenates onto agar plate. Total viable count and mesophilic anaerobic sporulating bacteria were determined on Plate Count Agar after incubation for 2 days at 37 °C. Pseudomonas aeruginosa enumeration was carried out after incubation of Pseudomonas Isolation agar at 48 h at 35 °C. For members of the family Enterobacteriaceae (45 °C Violet Red Bile Glucose agar were used and incubation was carried out for 24 h at 37 °C. For determinations of fungal colonies Malt agar and Czapek-Dox agar were inoculated using the spread-plate technique and incubated at 25 °C for 5 days. The yeasts were grown in Glucose Yeast Peptone agar (aerobiosis at 25 °C during 72 hours. The total viable count of fruits ranged from 4.07 log cfu.g-1 in Rišňovce to 4.84 log cfu.g-1 in Vrbové Baraní dvor. Number of mesophilic anaerobic sporulating bacteria ranged from 4.09 in Vrbové Baraní dvor to 4.82 log cfu.g-1 in Modrá pažiť. Number of Pseudomonas aeruginosa count ranged from 2.00 in Nitra Zobor and Vrbové Baraní dvor to 3.94 log cfu.g-1 in Modrá pažiť. In our study the number of Enterobacteriaceae genera ranged from 3.38 in Nitra Zobor to 4.25 log cfu.g-1 in Vrbové Baraní dvor. Number of yeasts ranged from 3.36 in Vrbové Baraní dvor to 3.85 log cfu.g-1 in Modrá pažiť. Number of microscopic filamentous fungi ranged from 2.60 in Modrá Pažiť to 3.52 log cfu.g-1 in Nitra Zobor. Our findings indicate that Rose plant is naturally associated with a variety of endophytic microorganisms

  16. Rose hip (Rosa canina L: A functional food perspective

    Directory of Open Access Journals (Sweden)

    Cui Fan

    2014-12-01

    Full Text Available Rose hip (Rosa canina L. is the pseudo-fruit of the rose plant, which is widely known as a valuable source of polyphenols and vitamin C. Both in vivo and in vitro studies have demonstrated that this fruit exhibits anti-inflammatory, antioxidant, and antiobesogenic activities. The health benefits of Rose hip (RH have been attributed to its wide range of bioactive compounds including the anti-inflammatory galactolipid: (2S-1,2-di-O-[(9Z,12Z,15Z-octadeca-9,12,15-trienoyl]-3-O-β-D galactopyranosyl glycerol (GOPO, vitamin C, phenolics, lycopene, lutein, zeaxanthin, and other carotenoids. As cyclooxygenase inhibitors, RH compounds may reduce the risk of cancer, heart disease, and various inflammatory conditions. The aim of this review is to present an overview of the functional, medical, and physiological properties of RH.

  17. Notas de pintura rupestre solutrense

    Directory of Open Access Journals (Sweden)

    Francisco JORDÁ CERDÁ

    2009-11-01

    Full Text Available Desde hace algún tiempo venimos sosteniendo, junto con otros prehistoriadores, la necesidad de revisar las fases establecidas para el desarrollo del arte rupestre cuaternario y su cronología. El sistema preconizado por Breuil tiene la necesaria flexibilidad para permitir las rectificaciones que el tiempo necesariamente irá imponiendo a raíz de la investigación moderna. De ahí que nuestras notas o reflexiones sean más que críticas que tiendan a desvalorizar el sistema actualmente admitido, simples rectificaciones orientadas en los nuevos puntos de vista, producto de los descubrimientos más recientes. En este sentido hemos de hacer patente la importancia que la cueva del Parpalló (Gandía, con una estratigrafía clara y definida y con una serie de hallazgos de arte mobiliar perfectamente datados, tiene para el estudio del arte rupestre hispanofrancés, del Paleolítico superior.

  18. Canine atopic dermatitis / Dermatite atópica canina

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    Thalita da Costa Teles

    2008-08-01

    Full Text Available Canine Atopic Dermatitis is a skin disease of genetic origin. The affected dog becomes sensible to antigens presents in the environment, developing a severe alergic, pruriginous reaction, which intervenes in the quality of life of the patient. Because of the genetic character, that is an illness that in most of the times has no cure, just control. The treatment in general is lifetime. Thus, some used drugs, for example the corticosteroides, might cause collateral effects when used for a long time, and might decrease the lifetime of the animals. By this way, the owner of the dog with atopia, must know about the complications of the disease, and occasional return of the clinical signs during the period of treatment. Therefore, this article has an objective of a review about Canine Atopic Dermatitis that is an increasing problem in the small animal practice.A Dermatite Atópica Canina é uma dermatopatia de origem genética. Os cães acometidos tornam-se sensíveis aos antígenos presentes no meio ambiente, desenvolvendo grave reação alérgica, pruriginosa, que interferem na qualidade de vida do paciente. Devido ao seu caráter genético, esta é uma doença que na maioria das vezes não tem cura, apenas controle. O tratamento em geral é vitalício. Assim sendo, algumas drogas utilizadas, a exemplo dos corticosteróides, podem causar efeitos colaterais que em longo prazo, são capazes de diminuir o período de vida do animal. Desta maneira, o proprietário do cão portador de atopia, precisa ser esclarecido em relação às complicações e provável recidiva dos sinais clínicos, durante o período de tratamento. Portanto, presente artigo tem como objetivo uma revisão sobre Dermatite Atópica Canina, que está se tornando um problema crescente na clínica de pequenos animais.

  19. Isolation of immunomodulatory triterpene acids from a standardized rose hip powder (Rosa canina L.)

    DEFF Research Database (Denmark)

    Saaby, Lasse; Jäger, Anna Katharina; Moesby, Lise

    2011-01-01

    A previously published systematic review and a metaanalysis have concluded that the consumption of standardized rose hip powder (Rosa canina L.) can reduce pain in osteoarthritis patients. Synovial inflammation has been suggested to play an important role in the pathogenesis of osteoarthritis...... and mainly to involve infiltration of the synovial membrane by macrophages. Therefore, the immunomodulatory effect of standardized rose hip powder of Rosa canina L. was investigated and active principles isolated using the Mono Mac 6 cell line as a model for human macrophages. Treatment of Mono Mac 6 cells...

  20. Immunobiology of visceral leishmaniasis

    Directory of Open Access Journals (Sweden)

    Susanne eNylén

    2012-08-01

    Full Text Available Visceral leishmaniasis (VL, commonly known as kala-azar, is caused by Leishmania donovani and Leishmania infantum (Leishmania chagasi in the Americas. These Leishmania species infect macrophages throughout the viscera, and parasites are typically found in the spleen, liver and bone marrow. Patients with active disease typically exhibit marked immunosuppression, lack reactivity to the Leishmania skin test (LST, a delayed type hypersensitivity test, and their peripheral blood mononuclear cells (PBMC fail to respond when stimulated with leishmanial antigens in vitro. However, most people infected with visceralizing species of Leishmania never develop disease. Understanding immune failure and the underlying immune mechanism that lead to disease as well as control of infection are key questions for research in this field. In this review we discuss immunological events described in human and experimental VL and how these can affect the outcome of infection.

  1. Thuya occidentalis CH12 como tratamento alternativo da papilomatose canina

    Directory of Open Access Journals (Sweden)

    F.F. QUEIROZ

    2015-01-01

    Full Text Available RESUMO A papilomatose é uma doença de natureza crônica e pode causar tumores epiteliais e na mucosa. Não possui predileção por sexo, raça ou idade, mas comumente aparece em animais jovens. As lesões papilares ocorrem principalmente na mucosa bucal, nos lábios, língua e da faringe, além destes, também são encontradas na forma ocular e cutânea. Este estudo teve como objetivo relatar a eficácia da Thuya occidentalis CH12, uma medicação homeopática de uso comum, no tratamento de cães com papilomatose. Os resultados positivos puderam ser vistos, em alguns casos, já na primeira semana do tratamento, em outros, após vinte dias, e o desaparecimento total das lesões, na maioria dos casos, ocorreu com quinze dias. Alicerçada no princípio da similitude, a homeopatia apóia-se na observação experimental de que toda substância capaz de provocar determinados sintomas em uma pessoa sadia, é capaz de curar estes mesmos sintomas em uma pessoa enferma. A Thuya occidentalis provou ser eficiente contra lesões de papilomavírus canina, e apresentou resultados rápidos e com um valor econômico acessível.

  2. Controle da leishmaniose visceral no município de Porteirinha, estado de Minas Gerais, no período de 1998 a 2003

    Directory of Open Access Journals (Sweden)

    Ricardo Andrade Barata

    2011-06-01

    Full Text Available INTRODUÇÃO: No município de Porteirinha, Estado de Minas Gerais, foram registrados 23 casos humanos de leishmaniose visceral (LV nos anos de 1998 e 1999. MÉTODOS: Foi realizado um estudo envolvendo a tríade de ações preconizadas no controle da LV. Pacientes com leishmaniose humana foram tratados e cães sorologicamente positivos foram eutanasiados, trimestralmente. O inseticida piretróide α-cipermetrina foi aplicado nos bairros onde casos humanos foram registrados. RESULTADOS: Houve uma redução da soroprevalência canina e de flebotomíneos capturados, após a implementação das medidas de controle, refletindo na diminuição de casos humanos de leishmaniose visceral. CONCLUSÕES: Os resultados mostraram a eficiência destas medidas quando empregadas em conjunto.

  3. Notas Ornitológicas Colombianas, IV

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    Dugand Armando

    1948-06-01

    Full Text Available La lista siguiente, cuarta en la serte de mis notas ornitológicas colombianas (*, contiene noticias de algún interés relativas a elementos propios de la avifauna de Colombia y a varias especies migratorias que se encuentran de manera temporal en el territorio de este país. En total, 178 especies y subespecies se enumeran en este trabajo.

  4. Inmunohistoquimica y expresion de receptores de estrogenos y progesterona en neoplasias mamarias malignas caninas en distintos estadios clinicos

    National Research Council Canada - National Science Library

    Hermo, Guillermo; Soldati, Rocio; Wargon, Victoria; Scursoni, Alejandra; Lanari, Claudia; Gobello, Cristina

    2010-01-01

    La tecnica de inmunohistoquimica descrita por Walker y col. (1998) y Mote y col. (2001) es de utilidad para la determinacion de receptores de estrogenos y de progesterona en tumors mamarios de la hembra canina...

  5. Samenspannen tegen XTC : Tussentijdse evaluatie van de XTC-Nota

    NARCIS (Netherlands)

    Neve, R.J.M.; Ooyen-Houben, M.M.J. van; Snippe, J.; Bieleman, B.; Kruize, A.; Bijl, R.V.

    2005-01-01

    De door de nota 'Samenspannen tegen XTC' ingezette beleidsinspanning wordt geëvalueerd met drie metingen. Dit rapport is het resultaat van de tweede meting (bestaande uit twee onderzoeksprojecten) ofwel 'tussenevaluatie' van de XTC-nota. Deze evalutie richt zich op de periode tot 2004. Daaruit

  6. Samenspannen tegen XTC : Eindevaluatie van de XTC-nota

    NARCIS (Netherlands)

    Neve, R.J.M.; Ooyen-Houben, M.M.J. van; Bieleman, B.; Snippe, J.

    2007-01-01

    Van 2001 tot eind 2006 worden activiteiten uitgevoerd in het kader van de nota ‘Samenspannen tegen XTC’. Doel van het in deze nota geformuleerde beleid is om door een versterking van de betrokken instanties en van de samenwerking tussen deze instanties een substantiële reductie in de productie van

  7. Mercado laboral en Chile: Nota técnica sectorial

    OpenAIRE

    Graciana Rucci

    2011-01-01

    La presente nota técnica presenta un panorama del mercado laboral chileno y su contexto a nivel nacional. Esta nota incluye un diagnóstico del sector, la programación del BID para el período 2010-2014 y una matriz de resultados.

  8. Visceral leishmaniasis in Brazil

    Directory of Open Access Journals (Sweden)

    Mary Marcondes

    2013-10-01

    Full Text Available Visceral leishmaniasis (VL is among the most important vector-borne diseases that occur in Brazil, mainly due to its zoonotic nature. It is currently present in almost all Brazilian territory, and its control is a challenge both for veterinarians and for public health officials. The etiologic agent is Leishmania infantum (syn chagasi, and the main vector in Brazil is Lutzomyia longipalpis. Of all animals identified as reservoirs of VL, the dog is considered the most important domestic reservoir. Although the disease has already been identified in cats, the epidemiological role of this animal species is still unclear. This article presents a brief review of the epidemiological situation of the disease, its mode of transmission, clinical features in dogs and cats as well as possible risk factors associated with the occurrence of the disease in Brazil.

  9. Avaliação das atividades de controle da leishmaniose visceral em Belo Horizonte, Minas Gerais, 2006-2011

    Directory of Open Access Journals (Sweden)

    Maria Helena Franco Morais

    Full Text Available OBJETIVO: avaliar as atividades de controle da leishmaniose visceral (LV em Belo Horizonte-MG, Brasil. MÉTODOS: estudo descritivo para avaliação do Programa de Vigilância e Controle da Leishmaniose Visceral pautado em seus objetivos; utilizaram-se dados dos Sistemas de Informação de Controle de Zoonoses e de Agravos de Notificação. RESULTADOS: entre 2007 e 2011, verificou-se adequação da estratégia de controle do reservatório com aumento da cobertura de áreas priorizadas (23,4% e da população canina examinada (43,3%, eutanásia dos cães sororreagentes superior a 85,0% e redução de 47,8% na soroprevalência canina; no período 2008-2011, observou-se redução na incidência de casos humanos de 7,2 para 3,9/100 mil habitantes; não houve ampliação da cobertura de áreas priorizadas para o controle do vetor. CONCLUSÃO: os indicadores de resultados demonstraram o alcance dos objetivos do programa, com diferente adequação entre as estratégias de controle; a complexidade da intervenção, porém, indica a necessidade de revisão das ações propostas.

  10. Effects of Rosa Canina L. on Ischemia/ Reperfusion Injury in Anesthetized Rats

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    S Karimi

    2012-04-01

    Full Text Available Background: Ischemia/reperfusion induced acute renal failure causes excretory functional disorders of nephrons. Ischemia/reperfusion injury is accompanied by generation of reactive oxygen species that leads to dysfunction, injury, and death of renal cells. Antioxidants of plant origin minimize the harmful effects of reactive oxygen species. The aim of this study was to determine the possible therapeutic potentials of Rosa canina L. in preventing renal functional disturbances during the post-ischemic reperfusion period. Methods: In this experimental study undertaken for evaluating renal excretory function in 30 male Wistar rats, renal ischemia was induced by occluding both renal arteries for 45 min, followed by 24 h of reperfusion. The rats received 2 ml of tap water or a hydroalcoholic extract of Rosa canina (500 mg/kg orally for 7 days before induction of ischemia. In plasma samples, creatinine and urea nitrogen levels were measured, and in renal tissue samples, red blood cells were counted. The data were analyzed using ANOVA and Duncan tests.Results: Renal ischemia for 45 minutes increased plasma levels of creatinine (P<0.001 and nitrogen urea (P<0.01 while reducing red blood cell counts in renal glomeruli (P<0.001. Rosa canina administration diminished the increase in creatinine (P<0.001 and nitrogen urea concentrations (P<0.01, and prevented reductions in red blood cell counts in renal glomeruli (P<0.001. Conclusion: Rosa canina seems to be useful as a preventive agent against renal damages induced by ischemia/reperfusion injuries in rats.

  11. ALTERNATIVAS PARA EL DIAGNÓSTICO DE LA PARVOVIROSIS CANINA EN HECES

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    S Ariza

    2005-01-01

    Full Text Available En vista de su alta morbilidad y letalidad, se han desarrollado varios métodos para el diagnóstico de la parvovirosis canina. Los más utilizados se basan en la detección de partículas virales excretadas en las heces durante la fase aguda de la enfermedad. Algunos requieren equipos especializados, lo que aumenta los costos y el tiempo de diagnóstico. Por esto, la simplificación de los métodos de diagnóstico con alta sensibilidad y especificidad es prioritaria. Este trabajo presenta el desarrollo de la técnica de aglutinación en látex para el diagnóstico de la parvovirosis canina en heces, comparada con un ELISA comercial y una hemoaglutinación. Sobre una población de 71 caninos con signos compatibles con parvovirosis canina, utilizando la prueba ELISA (SNAP® como referencia, se seleccionaron 21 caninos positivos. Empleando las muestras de materia fecal de los animales positivos se desarrollaron y compararon las pruebas de hemoaglutinación y aglutinación en látex. Se encontró una sensibilidad del 100% y una especificidad del 100% para aglutinación en látex y una sensibilidad del 95% y una especificidad del 100% para hemoaglutinación. Dada la sencillez de la prueba, los resultados obtenidos y sus costos, se recomienda la prueba de aglutinación en látex para el diagnóstico rápido y seguro de la parvovirosis canina.

  12. Estudo descritivo da estrutura populacional canina da área urbana de Araçatuba, São Paulo, Brasil, no período de 1994 a 2004 A descriptive profile of the canine population in Araçatuba, São Paulo State, Brazil, from 1994 to 2004

    Directory of Open Access Journals (Sweden)

    Andréa Maria Andrade

    2008-04-01

    Full Text Available No período de 1994 a 2004, a população canina de Araçatuba, São Paulo, Brasil, registrou duas importantes zoonoses: a raiva e a leishmaniose visceral. Analisaram-se as mudanças ocorridas nessa população durante esse período, utilizando resultados de censos caninos e de coletas censitárias de sangue realizados em 1994, 1999 e 2004. A relação cão/10 habitantes variou significativamente, passando de 1,7 em 1994 para 2,0 em 1999 e para 1,8 em 2004. A porcentagem de cães com até um ano de idade passou de 20% para 32,5% e o número de eutanásias realizadas também aumentou após 1999, com a introdução da leishmaniose visceral. O número de cães e a estrutura etária variaram nos diversos setores do município e aqueles com maior porcentagem de animais com até dois anos de idade apresentaram maior ocorrência de casos de leishmaniose visceral humana e canina. Tais resultados decorrem de ações de controle adotadas nos setores com casos humanos de leishmaniose visceral, porém, o aumento da população canina mais jovem pode resultar em aumento da susceptibilidade destes cães à doença, favorecendo a manutenção da mesma na área.From 1994 to 2004, the canine population in Araçatuba, São Paulo State, Brazil, suffered two major canine zoonoses: rabies and visceral leishmaniasis. Changes in the dog population during this period were evaluated using canine census data from 1994 and 2004 and the results of blood samples for diagnosis of canine visceral leishmaniasis in 1999. The ratio of dogs per 10 inhabitants varied from 1.7 in 1994 to 2.0 in 1999 and 1.8 in 2004. The percentage of puppies less than 1 year of age increased from 20% to 32.5%, and the number of euthanized dogs also increased after 1999, when visceral leishmaniasis began to appear. The number of dogs and percentage of puppies varied between different areas of the city, and neighborhoods with a higher percentage of young animals showed more cases of both human and

  13. Rosa canina Extracts Have Antiproliferative and Antioxidant Effects on Caco-2 Human Colon Cancer.

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    Sandra Jiménez

    Full Text Available The in vitro antiproliferative and antioxidant effects of different fractions of Rosa canina hips on human colon cancer cell lines (Caco-2 was studied. The compounds tested were total extract (fraction 1, vitamin C (fraction 2, neutral polyphenols (fraction 3 and acidic polyphenols (fraction 4. All the extracts showed high cytotoxicity after 72 h, both low and high concentrations. The flow cytometric analysis revealed that all the fractions produce disturbances in the cell cycle resulting in a concomitant cell death by an apoptotic pathway. Changes in the redox status of Caco-2 cells in response to Rosa canina hips were determined. Cells were exposed to hydrogen peroxide in presence of plant fractions and the production of Reactive Oxygen Species (ROS was significantly decreased. Therefore, our data demonstrate that rosehip extracts are a powerful antioxidant that produces an antiproliferative effect in Caco-2 cells. Therefore, these results predict a promising future for Rosa canina as a therapeutic agent. Thus, this natural plant could be an effective component of functional foods addressed towards colorectal carcinoma.

  14. Leishmaniose visceral no Brasil: quadro atual, desafios e perspectivas Visceral Leishmaniasis in Brazil: current status, challenges and prospects

    Directory of Open Access Journals (Sweden)

    Célia Maria Ferreira Gontijo

    2004-09-01

    Full Text Available No Brasil, a importância da leishmaniose visceral reside não somente na sua alta incidência e ampla distribuição, mas também na possibilidade de assumir formas graves e letais quando associada ao quadro de má nutrição e infecções concomitantes. A crescente urbanização da doença ocorrida nos últimos 20 anos coloca em pauta a discussão das estratégias de controle empregadas. Neste artigo foram analisados os principais aspectos biológicos, ambientais e sociais que influenciaram no processo de expansão e urbanização dos focos da doença. Os métodos disponíveis para o diagnóstico e tratamento não apresentam a eficácia e aplicabilidade desejadas, embora avanços promissores tenham sido alcançados com as pesquisas de novos testes diagnósticos e drogas terapêuticas. As medidas de controle da doença até agora implementadas foram incapazes de eliminar a transmissão e impedir a ocorrência de novas epidemias. É feita uma breve análise destas medidas e dos desafios a serem enfrentados. A prevenção da doença nos cães através da imunoprofilaxia aparece como uma alternativa para o controle. Uma nova vacina para cães, já testada em campo, está sendo industrializada e será comercializada no Brasil a partir de 2004. Apesar da existência de inúmeros estudos sobre a leishmaniose visceral humana e canina, muitas lacunas ainda precisam ser preenchidas.Visceral leishmaniasis has assumed an increasing importance in Brazil due to its high incidence and wide geographical distribution. When associated with malnutrition and co-infections it can prove to be fatal. A notable increase in transmission rates related to urbanization has been observed in the past 20 years. A combination of measures is needed to define new methods for reducing transmission. This paper analyzes the main biological, environmental and social aspects that have influenced the spread and urbanization of the disease. The diagnostic tests and drugs available

  15. [Visceral leishmaniasis: an update].

    Science.gov (United States)

    Faucher, B; Piarroux, R

    2011-09-01

    During the last decade, visceral leishmaniasis has been reconsidered in its epidemiology and strategies for diagnosis, treatment and prevention. This vectorial disease, responsible for more than 50,000 deaths each year across India, East Africa, South America, the Mediterranean area, Central Asia and China, is currently spreading over new territories. This formerly rural disease has even reached cities in South America. This spreading is caused by environmental changes due to global warming or human activity, and by the movement of workers and refugees. As a consequence, the burden of HIV/Leishmania coinfection is increasing in many developing countries even though effective antiretroviral therapy has led to a marked decrease in its incidence in Europe. The disease is now handled differently than it was 10 years ago: PCR has become the most accurate tool for diagnosis and follow-up in developed countries, and field diagnostic tools have been developed (antigenuria, rK39 dipstick). While resistance to antimoniate has appeared in India and Europe, new therapies have been evaluated such as miltefosine, the first oral therapy, or short treatment with liposomal amphotericin B. In France, liposomal amphotericin B has supplanted antimoniate meglumine because of better tolerance and shorter hospitalization duration. Protecting dogs through immunization or collars impregnated with deltamethrin proved effective to prevent zoonotic leishmaniasis due to Leishmania infantum. Copyright © 2010 Société nationale française de médecine interne (SNFMI). Published by Elsevier SAS. All rights reserved.

  16. Anterior herniation of lumbar disc induces persistent visceral pain: discogenic visceral pain: discogenic visceral pain.

    Science.gov (United States)

    Tang, Yuan-Zhang; Shannon, Moore-Langston; Lai, Guang-Hui; Li, Xuan-Ying; Li, Na; Ni, Jia-Xiang

    2013-01-01

    Visceral pain is a common cause for seeking medical attention. Afferent fibers innervating viscera project to the central nervous system via sympathetic nerves. The lumbar sympathetic nerve trunk lies in front of the lumbar spine. Thus, it is possible for patients to suffer visceral pain originating from sympathetic nerve irritation induced by anterior herniation of the lumbar disc. This study aimed to evaluate lumbar discogenic visceral pain and its treatment. Twelve consecutive patients with a median age of 56.4 years were enrolled for investigation between June 2012 and December 2012. These patients suffered from long-term abdominal pain unresponsive to current treatment options. Apart from obvious anterior herniation of the lumbar discs and high signal intensity anterior to the herniated disc on magnetic resonance imaging, no significant pathology was noted on gastroscopy, vascular ultrasound, or abdominal computed tomography (CT). To prove that their visceral pain originated from the anteriorly protruding disc, we evaluated whether pain was relieved by sympathetic block at the level of the anteriorly protruding disc. If the block was effective, CT-guided continuous lumbar sympathetic nerve block was finally performed. All patients were positive for pain relief by sympathetic block. Furthermore, the average Visual Analog Scale of visceral pain significantly improved after treatment in all patients (P herniation of the lumbar disc when forming a differential diagnosis for seemingly idiopathic abdominal pain. Continuous lumbar sympathetic nerve block is an effective and safe therapy for patients with discogenic visceral pain.

  17. Retos pendientes en el periodismo de nota roja

    Directory of Open Access Journals (Sweden)

    Francesc Barata

    2010-06-01

    Full Text Available La nota roja tiene que ser dignificada y tratada profesionalmente respetando los derechos de aquellos que tienen el desprecio social como una forma de fortalecer los valores democráticos. Un periodismo responsable y comprometido con los valores humanos de todos quienes piensen en que la nota roja puede develar muchas verdades ocultas de esta sociedad, aunque estén impregnadas de lodo y miseria.

  18. Samenspannen tegen XTC: Tussentijdse evaluatie van de XTC-Nota

    OpenAIRE

    Neve, R.J.M.; Ooyen-Houben, M.M.J. van; Snippe, J.; Bieleman, B.; Kruize, A; Bijl, R.V.

    2005-01-01

    De door de nota 'Samenspannen tegen XTC' ingezette beleidsinspanning wordt geëvalueerd met drie metingen. Dit rapport is het resultaat van de tweede meting (bestaande uit twee onderzoeksprojecten) ofwel 'tussenevaluatie' van de XTC-nota. Deze evalutie richt zich op de periode tot 2004. Daaruit blijkt dat de inbeslagnames van XTC-tabletten en ontmantelingen van XTC-laboratoria in Nederland in de periode 2002-2003 een dalende tendens laten zien, evenals het aantal in het buitenland gedetineerde...

  19. Neuropatologia da cinomose canina: 70 casos (2005-2008

    Directory of Open Access Journals (Sweden)

    Marcia C. Silva

    2009-08-01

    Full Text Available Este estudo teve como objetivo realizar uma investigação anátomo-patológica detalhada das lesões e sua distribuição no sistema nervoso central (SNC de cães com cinomose. Foram avaliadas secções padronizadas do encéfalo e da medula espinhal de 70 cães. Os casos foram agrupados de acordo com a idade dos cães e classificados conforme a evolução das lesões. Os resultados permitem concluir que: (1 encefalomielite induzida pelo vírus da cinomose canina é mais prevalente em filhotes e adultos; (2 lesões macroscópicas no SNC ocorrem com baixa freqüência; (3 o encéfalo é mais acometido do que a medula espinhal; (4 as cinco regiões anatômicas mais afetadas do encéfalo são, em ordem decrescente de freqüência, o cerebelo, o diencéfalo, o lobo frontal, a ponte e o mesencéfalo; (5 a região anatômica mais afetada da medula espinhal é o segmento cervical cranial (C1-C5; (6 lesões subagudas e crônicas são mais comuns do que lesões agudas; (7 desmielinização é a lesão mais prevalente e ocorre principalmente no cerebelo, na ponte e no diencéfalo, quase sempre acompanhada de astrogliose e inflamação não-supurativa; (8 na maior parte dos casos em que há astrogliose, observam-se astrócitos gemistocíticos, freqüentemente com formação de sincícios; (9 leptomeningite não-supurativa, malacia e necrose cortical laminar são lesões relativamente freqüentes no encéfalo, mas não na medula espinhal; (10 corpúsculos de inclusão no encéfalo são muito comuns, ocorrem principalmente em astrócitos e com freqüência menor em neurônios; no entanto, independentemente da célula afetada, são vistos predominantemente no núcleo; (11 uma classificação da encefalite na cinomose com base em síndromes clínicas relacionadas com a idade do cão é imprecisa.The current study was performed to determine the pathology and distribution of lesions in the central nervous system (CNS of 70 dogs naturally affected by canine

  20. Management of chronic visceral pain

    DEFF Research Database (Denmark)

    Olesen, Anne E; Farmer, Adam D; Olesen, Søren S

    2016-01-01

    Despite marked differences in underlying pathophysiology, the current management of visceral pain largely follows the guidelines derived from the somatic pain literature. The effective management of patients with chronic visceral pain should be multifaceted, including both pharmacological...... and psychological interventions, thereby providing a mechanism-orientated approach to treatment. Patients can frequently become disenfranchised, and subsequently disengaged, with healthcare providers leading to repeated consultations. Thus, a key aspect of management is to break this cycle by validating patients......' symptoms, adopting an empathic approach and taking time to educate patients. To optimize treatment and outcomes in chronic visceral pain we need to move away from approaches exclusively based on dealing with peripheral nociceptive input toward more holistic strategies, taking into account alterations...

  1. NOTAS PARA UMA ECLESIOLOGIA LATINO AMERICANA

    Directory of Open Access Journals (Sweden)

    Jesus Hortal

    1977-01-01

    Full Text Available Como o próprio título que me foi dado sugere, não se trata de algo perfeitamente elaborado, mas de uma série de notas, de pontos que querem provocar uma reflexão ulterior. Por isso, supõem os conceitos eclesiológicos fundamentais e deveriam incentivar a continuidade do pensamento eclesiológico no nosso meio. Por isso também, não me preocupei com a acumulação de bibliografia. Do que se trata não é de repetir o que os outros disseram, mas de abrir pistas para passar além do que já está dito. Daí que o meu pensamento fique, quase por completo, no plano metodológico.

  2. Treatment of visceral leishmaniasis

    Directory of Open Access Journals (Sweden)

    E M Moore

    2010-01-01

    Full Text Available The available treatment options for visceral leishmaniasis (VL have problems relating to efficacy, adverse effects and cost, making treatment a complex issue. We review the evidence relating to the different methods of treatment in relation to - efficacy and toxicity of the drugs in different areas of the world; ability to monitor side effects, length of treatment; ability of patients to pay for and stay safe during treatment, ability of the healthcare services to give intramuscular, intravenous or oral therapy; the sex and child-bearing potential of the patient and the immune status of the patient. The high mortality of untreated/ poorly treated VL infection makes the decisions paramount, but a unified and coordinated response by each area is likely to be more effective and informative to future policies than an ad hoc response. For patients in resource-rich countries, liposomal amphotericin B appears to be the optimal treatment. In South Asia, miltefosine is being used; the combination of single dose liposomal amphotericin B and short course miltefosine looks encouraging but has the problem of potential reproductive toxicities in females. In Africa, the evidence to switch from SSG is not yet compelling. The need to monitor and plan for evolving drug failure, secondary to leishmania parasite resistance, is paramount. With a few drugs the options may be limited; however, we await key ongoing trials in both Africa and India to explore the effects of combination treatment. If safe and reliable combinations are revealed by the ongoing studies, it is far from clear as to whether this will avoid leishmania parasite resistance. The development of new drugs to add to the armamentarium is paramount. Lessons can be learnt from the management of diseases such as tuberculosis and malaria in terms of planning the switch to combination treatment. As important as establishing the best choice for specific antileishmanial agent is ensuring treatment centers

  3. Associação da carga parasitária renal com achados laboratoriais em cães com leishmaniose visceral Renal parasite load association with laboratory findings in dogs with visceral Leishmaniasis

    Directory of Open Access Journals (Sweden)

    Mariana de Medeiros Torres

    2013-05-01

    Full Text Available A leishmaniose visceral canina é uma doença grave e a morte ocorre por falência renal, considerando que os métodos diagnósticos convencionais não possibilitam a classificação clínica do animal. O objetivo deste estudo foi associar a carga parasitária renal aos achados clínicos e histopatológicos em cães com leishmaniose visceral. A análise microscópica revelou predomínio de nefrite intersticial mononuclear de graus variados em 59,3% dos cães avaliados. Entretanto, não houve diferença entre a carga parasitária renal de sintomáticos e oligossintomáticos (P= 0,35. As lesões renais foram de ordem inflamatória e a quantidade de parasitos não influenciaram na característica dessas lesões e nem nas alterações bioquímicas, mesmo em cães com diferentes classificações clínicas.Canine visceral leishmaniasis is a severe disease and the death occurs from renal failure, whereas conventional diagnostic methods do not allow the animal clinical staging. The aim of this study was to associate the renal parasite load to clinical and histopathological findings in dogs with visceral Leishmaniasis. Microscopic analysis revealed a predominance of mononuclear interstitial nephritis of varying degrees in 59, 3% of dogs evaluated. However, no difference was found between the renal parasite load of symptomatics and oligosymptomatics (P= 0,35. Renal lesions were inflammatory order and amount of parasites not influenced the characteristics of theses lesions nor biochemical changes, even in dogs with different clinical classifications.

  4. Chemical composition of the epicuticular and intracuticular wax layers on adaxial sides of Rosa canina leaves.

    Science.gov (United States)

    Buschhaus, Christopher; Herz, Hubert; Jetter, Reinhard

    2007-12-01

    The waxy cuticle is the first point of contact for many herbivorous and pathogenic organisms on rose plants. Previous studies have reported the average composition of the combined wax extract from both sides of rose leaves. Recently, the compositions of the waxes on the adaxial and abaxial surfaces of Rosa canina leaves were determined separately. In this paper, a first report is made on the compositions of the epicuticular and intracuticular wax layers of Rosa canina leaves. The methods described enable the determination of which compounds are truly available at the surface for plant-organism interactions. An adhesive was used to mechanically strip the epicuticular wax from the adaxial leaf surface and the removal was visually confirmed using scanning electron microscopy. After the epicuticular wax had been removed, the intracuticular wax was then isolated using standard chemical extraction. Gas chromatography, flame ionization detection and mass spectrometry were used to identify and quantify compounds in the separated wax mixtures. The epicuticular wax contained higher concentrations of alkanes and alkyl esters but lower concentrations of primary alcohols and alkenols when compared to the intracuticular wax. In addition, the average chain lengths of these compound classes were higher in the epicuticular wax. Secondary alcohols were found only in the epicuticular layer while triterpenoids were restricted mainly to the intracuticular wax. A gradient exists between the composition of the epi- and intracuticular wax layers of Rosa canina leaves. This gradient may result from polarity differences, in part caused by differences in chain lengths. The outer wax layer accessible to the phyllosphere showed a unique composition of wax compounds. The ecological consequences from such a gradient may now be probed.

  5. Visceral leishmaniasis: an update of laboratory diagnosis

    Directory of Open Access Journals (Sweden)

    Zineb Tlamcani

    2016-07-01

    Full Text Available Visceral leishmaniasis, is an infection due to obligate intracellular protozoa of the genus Leishmania. There exist two varieties of visceral leishmaniasis, that vary in their transmission aspects: zoonotic visceral leishmaniasis and anthroponotic visceral leishmaniasis. Their clinical features are comparable with sevral differences. Laboratory diagnosis of visceral leishmaniasis consists of microscopic observation of parasite, culture from appropriate samples, detection of antigen, serological tests, and identification of parasite DNA. In this review, we will discuss the different techniques of diagnosis and the interet of the recent methods such as rapid diagnostic test and direct agglutination test.

  6. Aspectos moleculares del virus de la parvovirosis canina y sus implicaciones en la enfermedad

    OpenAIRE

    César A. Díaz R.; Jairo Jaime Correa; Víctor Julio Vera A.

    2008-01-01

    El parvovirus canino tipo 2 (CPV-2) es el agente causal de una enfermedad infecto-contagiosa que produce una gastroenteritis aguda hemorrágica que afecta a caninos jóvenes. El CPV-2 se adaptó a la especie canina por mutación del virus de la Panleucopenia felina (FPV) luego de su paso por animales silvestres como el hurón y los zorros. La alta variabilidad de la proteína viral 2 (VP2) es la causa principal del amplio rango de hospedadores y de las reacciones cruzadas entre las variantes. En la...

  7. Formas clínicas de la demodicosis canina. No todo son alopecias

    OpenAIRE

    Saló, E

    2011-01-01

    La demodicosis canina es una conocida y frecuente enfermedad cutánea, que puede afectar a los perros, causada por ácaros del género Demodex. Su clínica cutánea suele estar caracterizada por la presencia de eritema y alopecia, debido principalmente a la localización folicular del ácaro. Sin embargo, y de manera menos habitual, la enfermedad puede presentarse también con otras formas clínicas, junto o no con alopecia, pudiendo aparecer como síntomas principales o únicos de la enfermedad pequeño...

  8. Determination of antioxidant properties of Rumex crispus and Scrophularia canina subsp. bicolor

    OpenAIRE

    Demir, Serhat; Bozkurt, Buket; Önür, Mustafa Ali; Kaya, İrem Gülen; Ünver Somer, Nehir

    2017-01-01

    Methanoland ethyl acetate extracts of Rumex crispus L. and Scrophularia canina L. subsp. bicolor (SM.) Greuter were tested fortheir antioxidant activity using the DPPH method. Extracts were prepared fromthe above-ground parts of these plants. Significant antioxidant activity wasdetermined for methanol (IC50: 4.16 µg/mL) and ethyl acetate (IC50:8.71 µg/mL) extracts of Rumex crispus.Moreover, methanol (IC50: 60.78 µg/mL) and ethyl acetate (IC50:149.33 µg/mL) extracts of Scrophulariacanina subsp...

  9. Investigação epidemiológica de, um novo caso de leishmaniose visceral ocorrido na Grande São Paulo, Brasil Epidemiological investigation of a new case of visceral leishmaniasis in Greater S. Paulo, Brazil

    Directory of Open Access Journals (Sweden)

    Lygia Busch Iversson

    1982-08-01

    Full Text Available Descreve-se uma investigação epidemiológica realizada em zona urbana do município de São Paulo, Brasil, para esclarecer um caso de leishmaniose visceral ocorrido em criança de 2 anos de idade, nascida e sempre residente no local. Afastou-se a possibilidade de transmissão por via transfusional e por vetor biológico, tendo como base os dados levantados da anamnese do doente, os resultados de inquéritos realizados na área em população humana, utilizando testes de imunofluorescência indireta, hemaglutinação passiva e intradermoreação de Montenegro, em população canina com o teste de imunofluorescência indireta, além de pesquisa entomológica em mata residual.An epidemiological investigation was made in the urban zone of S. Paulo, Brazil, to elucidate a case of visceral leishmaniasis that occurred in a two-year old child, born and always resident in that place. The possibility of congenital transmission was eliminated. The transmission by blood transfusion and biological vector was discussed on the basis of the past history of the child and the results of a serological survery in human population with indirect imunofluorescence, passive hemagglutination and leishmanin tests, serological survey on dogs and furthermore entomological research in residual forest situated in the neighbourhood of the patient's house.

  10. Leishmaniose visceral: história jornalística de uma epidemia em Belo Horizonte, Brasil

    Directory of Open Access Journals (Sweden)

    Paula Dias Bevilacqua

    Full Text Available Este trabalho busca recuperar a história jornalística da epidemia de leishmaniose visceral que acomete o município de Belo Horizonte, Brasil, desde 1993. Este resgate se fez através da sua história cronológica e das construções narrativas e discursivas sobre a epidemia em 101 matérias publicadas na grande imprensa, durante o período de 1993 a 1996. Utilizando-se como orientação metodológica a proposta de Foucault (1972, em seu trabalho "A Arqueologia do Saber", a análise aponta para a configuração de três grandes discursos, caracterizando grupos temáticos, nas matérias estudadas: o da Ciência, o do Estado e o da Sociedade. Cada um dos grupos contribui para a construção da doença no espaço jornalístico e veicula opiniões próprias de cada um destes universos. Dessa forma, a leitura dos textos jornalísticos permite o desnudamento de outro processo epidêmico, que se desenvolve paralelamente àquele que acomete as populações humana e canina, expresso nas construções narrativas sobre a leishmaniose visceral nos textos de jornal.

  11. Volatile Oil Constituents of Rosa canina L.: Quality As Affected by the Distillation Method

    Directory of Open Access Journals (Sweden)

    Karim Hosni

    2010-01-01

    Full Text Available The volatile oils of R. canina flowers were isolated by hydrodistillation (HD and traditional dry distillation (DD and analyzed by HRGC-FID and GC-MS. Compared to HD, DD at 50°C leads to the isolation of high quality oil which contains the highest content of oxygenated compounds (83%. The main components are the 2-phenethyl alcohol and eugenol. The percentage of the 2-phenethyl alcohol, a highly desirable component in rose oil, was significantly higher (58.4% in DD extract when compared to that of HD one (13.6%. As temperature increased (100°C during DD, the oil quality decreased. The most significant changes were observed in 2-phenethyl alcohol percentage (4.5%. Moreover, an increase of alkanes/alkenes and the production of furan derivatives were observed. So, DD at moderate temperature (50°C seemed more suitable to improve the volatile oil quality and hence, to make more value of R. canina.

  12. Multiple Visceral and Peritoneal Anomalies

    Directory of Open Access Journals (Sweden)

    Gayathri Prabhu S

    2016-07-01

    Full Text Available Visceral and peritoneal anomalies are frequently encountered during cadaveric dissections and surgical procedures of abdomen. A thorough knowledge of the same is required for the success of diagnostic, surgical and radiological procedures of abdomen. We report multiple peritoneal and visceral anomalies noted during dissection classes for medical undergraduates. The anomalies were found in an adult male cadaver aged approximately 70 years. The right iliac fossa was empty due to the sub-hepatic position of caecum and appendix. The sigmoid colon formed an inverted “U” shaped loop above the sacral promontory in the median position. It entered the pelvis from the right side and descended along the lateral wall of the pelvis. The sigmoid mesocolon was attached obliquely to the posterior abdominal wall, just above the sacral promontory. Further there was a cysto-colic fold of peritoneum extending from the right colic flexure. We discuss the clinical significance of the variations.

  13. SOMATIC PAIN OF VISCERAL ORIGIN

    Science.gov (United States)

    Reggars, John W.

    1994-01-01

    It is not uncommon, within general practice, for patients to present with somatic or musculoskeletal pain of visceral origin. Furthermore patients may present with two separate and co-existing conditions within the same anatomical region making the clinical diagnosis confusing and complex. The following case study describes one such case which presented to a chiropractor. A discussion of examination findings, diagnostic dilemmas in such cases, differential diagnoses considered, diagnostic tests and appropriate therapy are discussed.

  14. Work experience at the Nota Bene Translation Agency

    OpenAIRE

    Kvashevich, Dzianis

    2017-01-01

    Dissertação de mestrado em Tradução e Comunicação Multilingue Obtaining the Master’s Degree in Translation and Multilingual Communication of the University of Minho (Braga, Portugal) requires doing a three-month internship as a part of the study programme. Particularly for this purpose I have worked as a translator-editor at the Nota Bene Translation Agency located in Minsk, Republic of Belarus. The Nota Bene Translation Agency (also known as Govorun prior to 2012) is a provider of turn...

  15. Samenspannen tegen XTC: Eindevaluatie van de XTC-nota

    OpenAIRE

    Neve, R.J.M.; Ooyen-Houben, M.M.J. van; Bieleman, B.; Snippe, J.

    2007-01-01

    Van 2001 tot eind 2006 worden activiteiten uitgevoerd in het kader van de nota ‘Samenspannen tegen XTC’. Doel van het in deze nota geformuleerde beleid is om door een versterking van de betrokken instanties en van de samenwerking tussen deze instanties een substantiële reductie in de productie van en handel in XTC te bewerkstelligen. In 2002 is de eerste meting van het evaluatieonderzoek van dit beleid uitgevoerd. In 2005 is de tussenmeting afgerond. In 2005/2006 is een eindmeting uitgevoerd....

  16. Immunity and immunosuppression in experimental visceral leishmaniasis

    OpenAIRE

    Goto H; Lindoso J.A.L.

    2004-01-01

    Leishmaniasis is a disease caused by protozoa of the genus Leishmania, and visceral leishmaniasis is a form in which the inner organs are affected. Since knowledge about immunity in experimental visceral leishmaniasis is poor, we present here a review on immunity and immunosuppression in experimental visceral leishmaniasis in mouse and hamster models. We show the complexity of the mechanisms involved and differences when compared with the cutaneous form of leishmaniasis. Resistance in viscera...

  17. The Protective Effect of Hydroalcoholic Extract of Rosa canina (Dog Rose) Fruit on Liver Function and Structure in Streptozotocin-Induced Diabetes in Rats.

    Science.gov (United States)

    Taghizadeh, Mohsen; Rashidi, Ali Akbar; Taherian, Ali Akbar; Vakili, Zarichehr; Mehran, Mehdi

    2017-11-02

    This study was conducted to evaluate the hepatoprotective effects of Rosa canina (R. canina) extract on streptozotocin- (STZ-) induced diabetes in rats by measuring the fasting blood glucose (FBG), total antioxidant capacity (TAC), and liver enzyme activity, including serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Forty adult male Wistar rats were randomly divided into four groups and treated daily for 42 days as follows: group I (control) received saline as a vehicle; group II (diabetic) received saline; groups III and IV (diabetic) treated with 250 and 500 mg/kg body weight (BW) per day R. canina extract, respectively. Diabetes was induced by a single intraperitoneal (IP) injection of streptozotocin (50 mg/kg BW). At the end of the study, blood samples were collected via heart puncture and sera were used for estimation of the mentioned parameters. Then all the rats were sacrificed and their livers used for histopathological evaluations. In the untreated diabetic group, the results showed a significant increase in FBG, ALT, and AST levels compared to the other groups (p .05). In the treated groups, administration of R. canina extract significantly improved the mentioned parameters in a dose-dependent manner (p < .05). Histological evaluations indicated that R. canina extract ameliorated defective liver caused by STZ. It can be concluded that R. canina extract has a hepatoprotective effect in STZ-induced diabetes in rats.

  18. Pathophysiology of human visceral obesity: an update

    National Research Council Canada - National Science Library

    Tchernof, André; Després, Jean-Pierre

    2013-01-01

    Excess intra-abdominal adipose tissue accumulation, often termed visceral obesity, is part of a phenotype including dysfunctional subcutaneous adipose tissue expansion and ectopic triglyceride storage...

  19. Notas sobre Afiloforales colombianos (Basidiomycetes: Aphyllophoralles Notas sobre Afiloforales colombianos (Basidiomycetes: Aphyllophoralles

    Directory of Open Access Journals (Sweden)

    Henao Luis Guillermo

    1989-09-01

    Full Text Available Some notes and a brief diagnostic description are provided for 19 species of Aphyllophorales. The following 15 species are new reports for the Colombian mycota: Thelephora palmata, Coltricia perennis, C. cinnamomea. Cyclomyces tabacinus, Phellinus rimosus, Dentinum repandum, Clavaria laeticolor, C. zollingeri, Polyporus dictyopus, Trametes membranaceus, T scabrosa, T. villosa, Trichaptum nebularis, Tyromyces duracinus and T. stipticus. Se proporcionan algunas notas y una descripción diagnóstica de 19 especies de Afiloforales colombianos. Las siguientes 15 especies se registran por primera vez en la micota colombiana: Thelephora pelmata, Coltricia perennis, C. cinnamomea, Cyclomyces tabacinus, Phellinus rimosus, Dentinum repandum, Clavaria laeticolor, C. zollingeri, Polyporus dictyopus, Trametes membranaceus, T. scabrosa, T villosa, Trichaptum nebuleris, Tyromyces duracinus y T stipticus.

  20. Notas para uma pronúncia do francês

    National Research Council Canada - National Science Library

    César Reis

    2011-01-01

    ... características individuais marcantes. Apresentamos, também, algumas notas sobre a pronúncia do francês. Palavras-chave: Língua francesa; fonologia; fonética; pronúncia; lingüística aplicada.Résumé: Nous proposons, dans cet article...

  1. Breve nota sobre tipos de corynebacterium diphteriae en Caracas

    Directory of Open Access Journals (Sweden)

    L. Briceño Iragorry

    1942-01-01

    Full Text Available Esta pequeña nota trata solamente de contribuir al mejor conocimiento de nuestras cepas de Corynebacterium diphteriae desde el punto de vista de sus propiedades culturales y relaciones con las formas clínicas de Difteria observadas en nuestro medio.

  2. Therapeutic options for visceral leishmaniasis.

    Science.gov (United States)

    Monge-Maillo, Begoña; López-Vélez, Rogelio

    2013-11-01

    Visceral leishmaniasis (VL), also known as Kala-Azar, is a disseminated protozoal infection caused principally by Leishmania donovani and Leishmania infantum (known as Leishmania chagasi in South America). The therapeutic options for VL are diverse and depend on different factors, such as the geographical area of the infection, development of resistance to habitual treatments, HIV co-infection, malnourishment and other concomitant infections. This article provides an exhaustive review of the literature regarding studies published on the treatment of VL, and gives therapeutic recommendations stratified according to their level of evidence, the species of Leishmania implicated and the geographical location of the infection.

  3. Investigation of Polyhenolic Content of Rose Hip (Rosa canina L. Tea Extracts: A Comparative Study

    Directory of Open Access Journals (Sweden)

    Zeynep İlbay

    2013-02-01

    Full Text Available Three different brands of Rose hip (Rosa canina L. tea were extracted with water, ethanol (EtOH, methanol (MeOH, and aqueous mixtures (50%, v/v by ultrasound-assisted extraction (UAE and Soxhlet methods. Total phenolic content was determined according to the Folin-Ciocalteu method. The results were presented by means of the extract yields and total phenolic contents, expressed in gallic acid equivalent (GAE per g of dried matter (DM. The greatest amount of extract observed in tea samples was obtained by UAE through water with the value of 619.37 ± 0.58 mg/g DM. Regarding the phenolic content, the best result was achieved by the Soxhlet method through 50% MeOH mixture (59.69 ± 0.89 mg GAE/g DM, followed by the UAE method with water (48.59 ± 0.29 mg GAE/g DM.

  4. Gel state of the fictional body in Mario Bellatin’s 'Rosa Canina' and "Flores"

    Directory of Open Access Journals (Sweden)

    Isabel Jasinski

    2015-12-01

    Full Text Available Mario Bellatin creates a peculiar "space of sense" in fiction, related to the visual and per­formatic expressions of language. So, his literary project presents writing as body language, a terri­tory of significance constantly deterritorialized by sensations connected to the narrative game, with images and symbols in the literary actions. A sense of creative destruction constitutes the paradox expressed by fiction as body and by the body as fiction, in what we call Bellatin’s "nomadic writing", by means of the abnormality of the characters, their sexuality and religiosity, as one can note in Flo­res (2000, especially in "Rosa canina", published online by Cosac Naify in 2009. The position adopted by the protagonist in this work seems to indicate a specific "regime of signification”, as Josefina Ludmer puts it, according to which art and reality, fiction and biography blur their borders in a “gel state exchange”.

  5. Investigation of Polyhenolic Content of Rose Hip (Rosa canina L.) Tea Extracts: A Comparative Study

    Science.gov (United States)

    İlbay, Zeynep; Şahin, Selin; Kırbaşlar, Ş. İsmail

    2013-01-01

    Three different brands of Rose hip (Rosa canina L.) tea were extracted with water, ethanol (EtOH), methanol (MeOH), and aqueous mixtures (50%, v/v) by ultrasound-assisted extraction (UAE) and Soxhlet methods. Total phenolic content was determined according to the Folin-Ciocalteu method. The results were presented by means of the extract yields and total phenolic contents, expressed in gallic acid equivalent (GAE) per g of dried matter (DM). The greatest amount of extract observed in tea samples was obtained by UAE through water with the value of 619.37 ± 0.58 mg/g DM. Regarding the phenolic content, the best result was achieved by the Soxhlet method through 50% MeOH mixture (59.69 ± 0.89 mg GAE/g DM), followed by the UAE method with water (48.59 ± 0.29 mg GAE/g DM). PMID:28239095

  6. Pathophysiology of stress-induced visceral hypersensitivity

    NARCIS (Netherlands)

    Stanisor, O.I.

    2014-01-01

    Visceral hypersensitivity is observed in the majority of patients with IBS and considered a pathophysiological mechanism. The aim of the work described in this thesis was to obtain a better understanding of the stress-related pathophysiology of visceral hypersensitivity. Investigations were carried

  7. Molecular diagnosis of visceral herpes zoster

    NARCIS (Netherlands)

    de Jong, M. D.; Weel, J. F.; van Oers, M. H.; Boom, R.; Wertheim-van Dillen, P. M.

    2001-01-01

    Patients with disseminated herpes zoster may present with severe abdominal pain that results from visceral involvement of varicella-zoster-virus infection. In the absence of cutaneous eruptions of herpes zoster, visceral herpes zoster is extremely difficult to diagnose. This diagnostic difficulty

  8. Drug Resistance in Visceral Leishmaniasis

    Directory of Open Access Journals (Sweden)

    Helena C. Maltezou

    2010-01-01

    Full Text Available Visceral leishmaniasis remains a public health problem worldwide. This illness was included by the World Health Organization in the list of neglected tropical diseases targeted for elimination by 2015. The widespread emergence of resistance to pentavalent antimonials in India where half cases occur globally and the unavailability of a vaccine in clinical use constitute major obstacles in achieving this goal. The last decade new antileishmanials became available, including the oral agent miltefosine. However, in poor endemic countries their wide use was curtailed because of the high costs, and also due to concerns of toxicity and emergence of resistance. Various mechanisms of antileishmanial resistance were identified recently in field isolates. Their elucidation will boost the design of new drugs and the molecular surveillance of resistance. Combination regimens should be evaluated in large trials. Overall, the development of antileishmanials has been generally slow; new drugs are needed. In order to control visceral leishmaniasis worldwide, treatment advances should become affordable in the poorest countries, where they are needed most.

  9. [Visceral leishmaniasis. Pediatric case report].

    Science.gov (United States)

    Gomila H, Andrés; Vanzo, Carolina; Garnero, Analía; Peruzzo, Luisina; Badalotti, Mónica

    2017-08-01

    La leishmaniasis es una enfermedad causada por parásitos obligados intracelulares pertenecientes al género Leishmania y que reconoce tres formas clínicas principales: cutánea, visceral y mucocutánea. Es una patología del grupo de las "enfermedades desatendidas". Es la única enfermedad tropical transmitida a través de vectores que se ha mantenido endémica por décadas en el sur de Europa. La leishmaniasis visceral representa la forma más grave. Se caracteriza por fiebre, pérdida de peso, anemia y hepatoesplenomegalia. Su período de incubación oscila entre 2 semanas y 18 meses. La leishmaniasis se considera una enfermedad reemergente a nivel mundial. Algunos de los factores que favorecen esta situación son los cambios en las condiciones climáticas, migraciones y urbanizaciones deficitarias en saneamiento ambiental. Se presenta el caso de un niño europeo que estaba vacacionando en Córdoba y fue derivado a nuestro Hospital por fiebre y pancitopenia, lo que generó un abordaje multidisciplinario con resolución clínica favorable. Sociedad Argentina de Pediatría.

  10. NOTAS PARA UN DIÁLOGO

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    María Nieves Alonso M.

    2004-11-01

    Full Text Available La relación de Pablo Neruda con la poesía y los poetas peninsulares ha sido destacada en varios estudios. El mismo poeta ha entregado hermosas señales sobre el asunto. En esta línea, "Notas para un diálogo" propone demostrar la vinculación profunda entre el Premio Nobel chileno y Antonio Gamoneda, poeta español (leonés nacido en Oviedo contemporáneo. La intuición inicial y los indicios preliminares, surgidos por la lectura de algunos relatos de infancia poetizados por los autores, permite avanzar hasta precisar los elementos de un diálogo temático, léxico y sintáctico que revela una misma proposición estética surgida de una conciencia desolada y en constante regreso a lo deshabitado del yo y con un gran compromiso con su tiempo. La profunda comunidad de la lengua que estos poetas habitan, compleja y diáfana a tiempos, muestra las afinidades y diferencias de unas poéticas que parecen decir: "Estamos rodeados de pura muerte, vivimos rodeados de muerte y, al mismo tiempo, de luz, palabras y silencio. Somos desiertos, pero poblados de tribus, de fauna, de flores"Pablo Neruda's relation to peninsular poetry and poets has been well emphasized in diverse studies. The poet himself has left beautiful intimations on this matter. In this perspective,'Notes for a Dialogue' intends to demostrate the prolound bonds between the Chilean Nobel Prize, and the contemporary Spanish poet, Antonio Gamonal (born in Oviedo. The initial intuition and the preliminary hints, arising from the reading of some childhood memories poetized by these poets, permit us to advance towards the identification of the elements of a syntactical, lexicographic and thematic dialogue revealing an identical aesthetic proposal born out of a desolate conscience in constant return to an uninhabited self, with full commitment to the age, The profound community of the language binding these poets, shows affinities as well as differences in their respective poetics which may

  11. Índices de sensibilidade à insulina em fêmeas caninas : efeito do ciclo estral e da piometra

    OpenAIRE

    Pöppl, Alan Gomes; Lasta, Camila Serina; Diaz Gonzalez, Felix Hilario; Kucharski, Luiz Carlos Rios; Silva, Roselis Silveira Martins da

    2009-01-01

    A ocorrência do estro e diestro em fêmeas caninas está associada à presença de resistência à insulina. Além disso, estados sépticos e inflamatórios, como observado frente à piometra, também cursam com resistência à ação insulínica. O objetivo deste estudo foi verificar qual o efeito das diferentes fases do ciclo estral e da piometra sobre diferentes índices de sensibilidade à insulina. Com base em medidas de glicemia e insulinemia basal em fêmeas caninas em diferentes fases do ciclo estral, e...

  12. The fate of ribosomal RNA genes in spontaneous polyploid dogrose hybrids (Rosa L. sect. Caninae (DC.) Ser.) exhibiting non-symmetrical meiosis.

    Science.gov (United States)

    Herklotz, Veit; Kovařík, Aleš; Lunerová, Jana; Lippitsch, Susan; Groth, Marco; Ritz, Christiane M

    2018-01-31

    Dogroses represent an exceptional system for studying the effects of genome doubling and hybridization: their asymmetrical meiosis enables recombination in bi-parentally inherited chromosomes but prevents it in maternally inherited ones. We employed fluorescent in situ hybridization, genome skimming, amplicon sequencing of genomic and cDNA as well as conventional cloning of nuclear ribosomal DNA in two phylogenetically distinct pentaploid (2n = 5x = 35) species, Rosa canina and R. inodora, and their naturally occurring reciprocal hybrids, R. dumalis (5x) and R. agrestis (5x, 6x). Both progenitor species differed in composition, meiotic behaviour and expression of rDNA loci: R. canina (five 18S and 5-8 5S loci) was dominated by the Canina ribotypes but R. inodora (four 18S loci and 7-8 5S loci) by the Rubiginosa ribotype. The co-localised 5S/18S loci occurred on either bivalent-forming (R. canina) or univalent-forming (R. inodora) chromosomes. Ribosomal DNA loci were additively inherited, however, the Canina ribotypes were dominantly expressed, even in genotypes with relatively low copy number of these genes. Moreover, we observed rDNA homogenization towards the paternally transmitted Canina ribotype in 6x R. agrestis. The here observed variation in arrangement and composition of rDNA types between R. canina and R. inodora suggests involvement of different genomes in bivalent formation. We hypothesize that the asymmetrical meiosis arose at least twice by independent ancient hybridization events. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

  13. Prevalência e epidemiologia da leishmaniose visceral em cães e humanos, na cidade de Cuiabá, Mato Grosso, Brasil Prevalence and epidemiology of visceral Leishmaniasis in dogs and humans in the city Cuiaba, Mato Grosso, Brazil

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    Arleana do Bom Parto Ferreira de Almeida

    2010-07-01

    Full Text Available A leishmaniose visceral é uma doença infecciosa endêmica em diversas regiões do Brasil, incluindo o Estado de Mato Grosso. Em Cuiabá, no período de janeiro de 2006 a dezembro de 2008, foi realizada investigação dos casos caninos de infecção por Leishmania sp. por meio de sorologia e citologia, enfocando a sua distribuição geográfica e correlacionando a ocorrência de casos de leishmaniose visceral humana. De 150 cães com suspeita da infecção, 57 (38% foram sorológicos ou parasitologicamente positivos. Observou-se soroprevalência significativa (P≤0,05 nos bairros com renda per capita baixa, além de maior número de casos caninos na regional leste; no entanto, observou-se uma distribuição difusa da doença canina na cidade de Cuiabá, enquanto os casos humanos originaram principalmente na regional norte do município. Esse fato sugere que há risco em todo o município, devendo ser realizados novos estudos soroepidemiológicos, assim como a distribuição vetorial, que promovam maior conhecimento da infecção canina por Leishmania sp, de forma a se gerar medidas adequadas para o controle da doença.Visceral Leishmaniasis is an infectious disease endemic in several regions of Brazil, including the state of Mato Grosso. In Cuiabá, from January 2006 to December 2008, a research was performed in canine cases of infection with Leishmania sp. by serology and cytology, focusing on its geographical distribution, correlating to the occurrence of human visceral Leishmaniasis cases. From 150 dogs with suspected infection, 57 (38% were serological or parasitologically positive. Seroprevalence was observed (P≤0.05 in districts with low per capita income, besides a larger number of canine cases in the East regional, but there was a diffuse distribution of the canine disease in the city of Cuiabá, in contrast to cases focused mainly on human in the northern municipality. This suggests risk throughout the municipality and further

  14. Multiple and asymmetrical origin of polyploid dog rose hybrids (Rosa L. sect. Caninae (DC.) Ser.) involving unreduced gametes.

    Science.gov (United States)

    Herklotz, V; Ritz, C M

    2017-08-01

    Polyploidy and hybridization are important factors for generating diversity in plants. The species-rich dog roses ( Rosa sect. Caninae ) originated by allopolyploidy and are characterized by unbalanced meiosis producing polyploid egg cells (usually 4 x ) and haploid sperm cells (1 x ). In extant natural stands species hybridize spontaneously, but the extent of natural hybridization is unknown. The aim of the study was to document the frequency of reciprocal hybridization between the subsections Rubigineae and Caninae with special reference to the contribution of unreduced egg cells (5 x ) producing 6 x offspring after fertilization with reduced (1 x ) sperm cells. We tested whether hybrids arose by independent multiple events or via a single or few incidences followed by a subsequent spread of hybrids. Population genetics of 45 mixed stands of dog roses across central and south-eastern Europe were analysed using microsatellite markers and flow cytometry. Hybrids were recognized by the presence of diagnostic alleles and multivariate statistics were used to display the relationships between parental species and hybrids. Among plants classified to subsect. Rubigineae , 32 % hybridogenic individuals were detected but only 8 % hybrids were found in plants assigned to subsect. Caninae . This bias between reciprocal crossings was accompanied by a higher ploidy level in Rubigineae hybrids, which originated more frequently by unreduced egg cells. Genetic patterns of hybrids were strongly geographically structured, supporting their independent origin. The biased crossing barriers between subsections are explained by the facilitated production of unreduced gametes in subsect. Rubigineae . Unreduced egg cells probably provide the highly homologous chromosome sets required for correct chromosome pairing in hybrids. Furthermore, the higher frequency of Rubigineae hybrids is probably influenced by abundance effects because the plants of subsect. Caninae are much more abundant

  15. Caracterização de casos de agressão canina em Campinas, São Paulo, Brasil

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    Ricardo Conde Alves Rodrigues

    2013-06-01

    Full Text Available Foi realizado um estudo retrospectivo com os dados sobre casos de agressão canina notificados em 2009 em Campinas, São Paulo, Brasil. Foram obtidas informações de 87 (3,8% entre 2.281 casos. A incidência acumulada de agressões foi de 2,42% por ano (IC95%: 2,32-2,51 para cada mil habitantes. A maior parte das vítimas de cães agressores eram homens (53,0%, adultos (72,0% e os próprios proprietários (52,9%. Os ataques ocorreram principalmente na rua (55,2%, enquanto a vítima interagia com o cão (79,3% e os membros superiores foram a parte do corpo mais acometida (49,4%. Os cães eram majoritariamente machos (74,7%, adultos (74,3%, sem raça definida (65,5%, não castrados (98,9%, domiciliados (55,2%, sem adestramento (98,1% e a metade deles já havia causado agressão com mordedura. A agressão canina não foi atribuída a sexo, raça, estado reprodutivo, tipo de restrição, ou adestramento dos animais, pois a distribuição da frequência destas variáveis na população canina do município era desconhecida. Para desenvolver protocolos de prevenção de mordeduras, devem ser realizados estudos populacionais para avaliar as características e a prevalência da agressão canina.

  16. [A case report of visceral leishmaniasis].

    Science.gov (United States)

    Popov, G; Andreeva, N; Georgiev, A; Kuzmanova, E

    1991-01-01

    A brief review of the spread of visceral leishmaniasis in Bulgaria and data about the disease are presented. A case of probably nonautochtonous form of visceral leishmaniasis is described. The clinical evolution of the disease is followed up and the large number of examinations which were carried out for the etiological diagnosis are presented. The diagnosis is based on the characteristic clinical symptoms, epidemiological history, positive serologic tests but with a negative myelogram. The diagnosis was proved ex juvantibus by the antimony treatment and the full recovery of the patient. This is the first case of visceral leishmaniasis in the 40 year history of the hospital.

  17. Nota sobre el Ejemplar Tipo de "Bromus catharticus" Vahl

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    Pinto Escobar Polidoro

    1976-09-01

    Full Text Available EI agrostólogo inglés C. E. Hubbard y simultáneamente el agrostólogo argentino Lorenzo Parodi en sendas notas (1956 concluyeron en declarar dudosa la especie Bromus catharticus Vahl y restablecer para la planta conocida como "rescue grass" en USA y "cebadilla criolla" en la Argentina el nombre Bromus unioloides H. B. K. Este trabajo presenta nuevas evidencias que permiten restablecer la validez de Bromus catharticus Vahl.

  18. Visceral leishmaniasis complicating systemic lupus erythematosus

    OpenAIRE

    Wallis, P. J. W.; Clark, C. J. M.

    1983-01-01

    Active systemic lupus erythematosus in a 32-year-old Chinese woman was successfully controlled by plasmapheresis and steroids. However, occult visceral leishmaniasis was uncovered during therapy and responded to appropriate treatment.

  19. Juvenile cellulitis in a puppy – case reportsCelulite juvenil canina – relato de casos

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    Carlos Eduardo Fonseca-Alves

    2012-08-01

    Full Text Available Juvenile cellulitis or juvenile sterile granulomatous lymphadenitis is a rare disorder that affects puppies between three weeks to six months years old. Clinical signs include alopecia, edema, papules, pustules and crusts especially on eyelids. Definitive diagnosis requires cytological and histological evaluation and early and aggressive therapy is recommended, once scars after recovery can be severe .The choice treatment is the high dose of corticosteroids use such as prednisone. Three animals of canine species were attended at the Veterinary Hospital Clinical Small Animal Service presenting different clinical signs. Hemogram, skin lesions and submandibular lymph nodes cytological examination was collected and analyzed. The treatment was instituted, using cephalexin (22mg/kg, twice daily up to control of secondary bacterial infection, and prednisone (2mg/kg, once a day until clinical resolution. Complete cure was obtained at the end of treatment. The aim of this work is to report three clinical cases of juvenile cellulitis in dogs. A celulite juvenil ou linfadenite granulomatosa estéril juvenil, é um distúrbio raro que acomete filhotes com idade entre três semanas a seis meses. Os sinais clínicos incluem alopecia, edema, pápulas, pústulas e crostas especialmente em pálpebras. O diagnóstico definitivo requer exames citológico e histopatológico e a terapia precoce e agressiva é preconizada, pois as cicatrizes após recuperação podem ser graves. O tratamento de escolha consiste no uso de altas doses dos glicocorticoides, como prednisona. Foram atendidos no Hospital Veterinário, três animais da espécie canina, apresentando sinais clínicos variados. Foram realizados hemograma, exame citológico das lesões de pele e dos linfonodos submandibulares. O tratamento foi instituído, utilizando-se cefalexina na dose de 22 mg/kg, duas vezes ao dia, até controle de infecção bacteriana secundária e prednisona na dose de 2mg/kg, uma vez

  20. Inspannen tegen XTC : Eerste meting: stand van zaken 2002 nota 'Samenspannen tegen XTC'

    NARCIS (Netherlands)

    Snippe, J.; Kruize, A.; Bieleman, B.

    2003-01-01

    Doel van dit onderzoek is na te gaan hoe het staat met de inspanningen en de resultaten van de aanpak van synthetische drugs/XTC in 2002, het beginjaar van de intensivering van de aanpak volgens de nota “Samenspannen tegen XTC”? Het betreft de baselinemeting van het evaluatieonderzoek. De nota

  1. Mechanisms of Stress-induced Visceral Pain

    OpenAIRE

    Greenwood-Van Meerveld, Beverley; Johnson, Anthony C

    2018-01-01

    Evidence suggests that long-term stress facilitates visceral pain through sensitization of pain pathways and promotes chronic visceral pain disorders such as the irritable bowel syndrome (IBS). This review will describe the importance of stress in exacerbating IBS-induced abdominal pain. Additionally, we will briefly review our understanding of the activation of the hypothalamic-pituitary-adrenal axis by both chronic adult stress and following early life stress in the pathogenesis of IBS. The...

  2. Aspectos moleculares del virus de la parvovirosis canina y sus implicaciones en la enfermedad

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    César A. Díaz R.

    2008-06-01

    Full Text Available El parvovirus canino tipo 2 (CPV-2 es el agente causal de una enfermedad infecto-contagiosa que produce una gastroenteritis aguda hemorrágica que afecta a caninos jóvenes. El CPV-2 se adaptó a la especie canina por mutación del virus de la Panleucopenia felina (FPV luego de su paso por animales silvestres como el hurón y los zorros. La alta variabilidad de la proteína viral 2 (VP2 es la causa principal del amplio rango de hospedadores y de las reacciones cruzadas entre las variantes. En la actualidad, la secuenciación de esta proteína ha permitido identificar tres variantes del virus conocidas como 2a, 2b y 2c que conviven en el mundo con diferencias en tropismo celular, infecciocidad y patogenicidad. El virus ssADN ha presentado una gran variación génica en cortos períodos de tiempo lo que indica un alto grado de selección por evolución sólo comparable con virus RN, esta alta variabilidad no se ha aclarado totalmente. El empleo de las técnicas moleculares permitirá diferenciar entre cepas vacunales y de campo, tanto como contar con técnicas diagnósticas confiables y específicas.

  3. Visceral Adiposity and Sarcopenic Visceral Obesity are Associated with Poor Prognosis After Resection of Pancreatic Cancer.

    Science.gov (United States)

    Okumura, Shinya; Kaido, Toshimi; Hamaguchi, Yuhei; Kobayashi, Atsushi; Shirai, Hisaya; Yao, Siyuan; Yagi, Shintaro; Kamo, Naoko; Hatano, Etsuro; Okajima, Hideaki; Takaori, Kyoichi; Uemoto, Shinji

    2017-11-01

    Visceral fat accumulation and muscle depletion have been identified as poor prognostic factors for various cancers. However, the significance of visceral adiposity and sarcopenic visceral obesity on outcomes after resection of pancreatic cancer remains unclear. A retrospective analysis of 301 patients who underwent resection for localized pancreatic cancer between 2004 and 2015 was performed. The extent of visceral adiposity [visceral to subcutaneous adipose tissue area ratio (VSR)] and visceral obesity [visceral fat area (VFA)] were measured on preoperative computed tomography images, together with skeletal muscle index (SMI) and muscle attenuation (MA). The impacts of these body composition parameters on outcomes after pancreatic resection were investigated. The overall survival (OS) and recurrence-free survival (RFS) rates in patients with high VSR were significantly lower than those in patients with low VSR (P = 0.001, P = 0.007, respectively). There were no differences in OS and RFS between high VFA and low VFA group; however, when analyzed together with sarcopenic factors, OS and RFS rates of the patients with sarcopenic visceral obesity were significantly lower compared with those of the others. Multivariate analyses revealed that high VSR was an independent risk factor for mortality (hazard ratio (HR) 1.58, P = 0.009) and recurrence (HR 1.41, P = 0.026) together with low SMI, low MA, high CA19-9, microvascular invasion, and nodal metastasis. Visceral adiposity and sarcopenic visceral obesity, as well as low muscle mass and quality, were closely associated with mortality and recurrence after resection of pancreatic cancer.

  4. Aplicaciones clínicas de los agonistas dopaminérgicos en reproducción canina

    OpenAIRE

    Gobello, Cristina

    2000-01-01

    Este artículo de actualización describe las principales indicaciones de los agonistas dopaminérgicos (AD) en reproducción canina y explica brevemente sus mecanismos de acción. Los AD son alcaloides de la ergotamina, derivados de síntesis de la ergolina que inhiben la secreción de prolactina regulando la acción dopaminérgica o la serotoninérgica. Los AD con los que se cuenta con mayor experiencia en medicina veterinaria de pequeños animales son la bromocriptina, la cabergolina y la metergolina...

  5. Diagnóstico por imagen de la articulación de la rodilla en la especie canina

    OpenAIRE

    Soler Laguía, Marta

    2003-01-01

    En la especie canina, la rodilla es la articulación que más frecuentemente presenta alteraciones, siendo las patologías de los ligamentos y meniscos las más comunes, asociadas a cambios degenerativos articulares secundarios. Para evaluar las estructuras que componen la rodilla y poder diagnosticar dichas alteraciones podemos emplear numerosas técnicas de diagnóstico por imagen como la artrografia, ecografía así como estudios de imagen por resonancia magnética (IRM). La artrografia de la rodil...

  6. Estudo sero-epidemiológico da Leishmaniose canina na Região da Cova da Beira

    OpenAIRE

    Coelho, S; Cardoso,L.; Brancal, H.; Martins, M.V.; Matos, A.C.

    2005-01-01

    Poster apresentado no IX Congresso Ibérico de Parasitologia que decorreu em Coimbra, na Universidade, de 25 a 28 de Outubro de 2005. A leishmaniose canina é uma zoonose parasitária transmitida quase exclusivamente através da picada de minúsculos insectos parasitados, do género Phlebotomus. Actualmente assume-se como um importante problema não só do ponto de vista veterinário como também de saúde pública (Martínez-Moreno, et al., 1999; Cardoso et al., 2004). Dado o facto d...

  7. [Sleep, emotions and the visceral control].

    Science.gov (United States)

    Pigarev, I N; Pigareva, M L

    2013-01-01

    It is known that sleep is connected with sensory isolation of the brain, inactivation of the consciousness and reorganization of the electrical activity in all cerebral cortical areas. On the other hand, sleep deprivation leads to pathology in visceral organs and finally to the death of animals, while there are no obvious changes in the brain itself. It stays the opened question how the changes in the brain activity during sleep could be con- nected with the visceral health? We proposed that the same brain areas and the same neurons, which in wakefulness process the information coming from the distant and proprioreceptors, switch during sleep to the processing of the interoceptive information. Thus, central nervous system is involved into the regulation of the life support functions of the body during sleep. Results of our experiments supported this hypothesis, explained many observations obtained in somnology and offered the mechanisms of several pathological states connected with sleep. However, at the present level of the visceral sleep theory there were no understanding of the well known link between the emotional states of the organisms and transition from wakefulness to sleep, and sleep quality. In this study the attempt is undertaken to combine the visceral theory of sleep with the need- informational theory ofemotions, proposed by P. Simonov. The visceral theory of sleep proposes that in living organisms there is a constant monitoring of the correspondence of the visceral parameters to the genetically determined values. Mismatch signals evoke the feeling of tiredness and the need of sleep. This sleep need en- ters the competition with the other actual needs of the organism. In according with the theory of P. Simonov emotions connected with a particular need play important role in their ranking for satisfaction. We propose that emotional estimation of the sleep need, based on the visceral signals, is realized in the same brain structures which undertake this

  8. [Treatment of visceral leishmaniasis in children].

    Science.gov (United States)

    Minodier, P; Noël, G; Blanc, P; Uters, M; Retornaz, K; Garnier, J M

    2007-02-01

    Visceral Leishmania infantum leishmaniasis is endemic in the south of France. For many years the mainstay for treatment of infected children was pentavalent antimony: meglumine antimoniate (Glucantime) or sodium stibogluconate (Pentostam). However these drugs are poorly tolerated and resistance similar to that observed in the treatment of Indian visceral Leishmania donovani leishmaniasis has been reported. Currently liposomal amphotericin B is being used instead of antimony for treatment of visceral leishmaniasis in children in France. In addition to being well tolerated, liposomal amphotericin B is almost 100% effective. It can be administered in six intravenous injections of 3-4 mg/kg each (days 1 to 5 then day 10). A two-day protocol (10 mg/kg/d) that would reduce overall cost by shortening the duration of hospitalization is now being studied. Another oral drug, i.e., miltefosine, has been successfully used for treatment of visceral leishmaniasis in India. However it has not been evaluated for treatment of Mediterranean visceral leishmaniasis.

  9. Correlation between Complicated Diverticulitis and Visceral Fat

    Science.gov (United States)

    Jeong, Jong Heon; Kim, Jin Ok; Tae, Hye Jin; Jung, Suk Hyun; Lee, Kang Nyeong; Jun, Dae Won; Lee, Oh Young; Yoon, Byung Chul; Choi, Ho Soon; Hahm, Joon Soo; Song, Soon Young

    2011-01-01

    The aim of this study was to examine the relationship of complications related to diverticulitis and visceral obesity. The study was based on a retrospective case note review conducted at the Hanyang University Hospital. Patients were diagnosed with diverticulitis based on clinical symptoms and abdominal computed tomography (CT) findings and divided into two groups: those admitted with complicated diverticulitis and those with a simple diverticulitis episode. We compared the body mass index (BMI) and degree of visceral obesity, measured by abdominal CT. The study included 140 patients, 87 (62.1%) were simple diverticulitis and 53 (37.9%) were complicated diverticulitis. In the complicated diverticulitis group, 9 (6.4%) cases were recurrent, 29 (20.7%) were perforation or abscess patients, and 28 (20%) were patients with systemic inflammatory response syndrome (SIRS). Of the SIRS patients, 13 were involved in other complication groups. When comparing in the two groups, the complicated diverticulitis group had a significantly higher visceral fat area (128.57 cm2 vs 102.80 cm2, P = 0.032) and a higher ratio of visceral fat area/subcutaneous fat area (0.997 vs 0.799, P = 0.014). Visceral obesity is significantly associated with complications of diverticulitis. PMID:22022188

  10. Vaccines for visceral leishmaniasis: A review.

    Science.gov (United States)

    Jain, Keerti; Jain, N K

    2015-07-01

    Visceral leishmaniasis, which is also known as Kala-Azar, is one of the most severely neglected tropical diseases recognized by the World Health Organization (WHO). The threat of this debilitating disease continues due to unavailability of promising drug therapy or human vaccine. An extensive research is undergoing to develop a promising vaccine to prevent this devastating disease. In this review we compiled the findings of recent research with a view to facilitate knowledge on experimental vaccinology for visceral leishmaniasis. Various killed or attenuated parasite based first generation vaccines, second generation vaccines based on antigenic protein or recombinant protein, and third generation vaccines derived from antigen-encoding DNA plasmids including heterologous prime-boost Leishmania vaccine have been examined for control and prevention of visceral leishmaniasis. Vaccines based on recombinant protein and antigen-encoding DNA plasmids have given promising results and few vaccines including Leishmune®, Leishtec, and CaniLeish® have been licensed for canine visceral leishmaniasis. A systematic investigation of these vaccine candidates can lead to development of promising vaccine for human visceral leishmaniasis, most probably in the near future. Copyright © 2015 Elsevier B.V. All rights reserved.

  11. Visceral pain hypersensitivity in functional gastrointestinal disorders.

    Science.gov (United States)

    Farmer, A D; Aziz, Q

    2009-01-01

    Functional gastrointestinal disorders (FGIDs) are a highly prevalent group of heterogeneous disorders whose diagnostic criteria are symptom based in the absence of a demonstrable structural or biochemical abnormality. Chronic abdominal pain or discomfort is a defining characteristic of these disorders and a proportion of patients may display heightened pain sensitivity to experimental visceral stimulation, termed visceral pain hypersensitivity (VPH). We examined the most recent literature in order to concisely review the evidence for some of the most important recent advances in the putative mechanisms concerned in the pathophysiology of VPH. VPH may occur due to anomalies at any level of the visceral nociceptive neuraxis. Important peripheral and central mechanisms of sensitization that have been postulated include a wide range of ion channels, neurotransmitter receptors and trophic factors. Data from functional brain imaging studies have also provided evidence for aberrant central pain processing in cortical and subcortical regions. In addition, descending modulation of visceral nociceptive pathways by the autonomic nervous system, hypothalamo-pituitary-adrenal axis and psychological factors have all been implicated in the generation of VPH. Particular areas of controversy have included the development of efficacious treatment of VPH. Therapies have been slow to emerge, mainly due to concerns regarding safety. The burgeoning field of genome wide association studies may provide further evidence for the pleiotropic genetic basis of VPH development. Tangible progress will only be made in the treatment of VPH when we begin to individually characterize patients with FGIDs based on their clinical phenotype, genetics and visceral nociceptive physiology.

  12. Notas de leitura | Homenagem a Anna Rachel Machado

    OpenAIRE

    Ernica,Mauricio; Lousada, Eliane; Cristóvão, Vera Lúcia Lopes; TONELLI, Juliana Reichert Assunção; Tognato, Maria Izabel Rodrigues; Beato-Canato, Ana Paula Marques; Silva, Alessandra Augusta Pereira da; Santos, Luiza Guimarães; Rocha, Suélen Maria; Dantas-Longhi, Simone Maria

    2012-01-01

    Em 20 de maio de 2012 faleceu Anna Rachel Machado, professora da PUC-SP desde 1979 com extenso trabalho de docência, pesquisa e intervenção nos domínios do ensino de língua e da análise do trabalho docente. Em reconhecimento à importância de sua obra, Cadernos Cenpec lhe presta homenagem publicando uma breve nota biográfica e apresentando suas principais obras.Anna Rachel gostava de ser reconhecida como professora, acima de tudo. Com o esforço de guardar um pouco de seu espírito, essa homenag...

  13. Notas suicidas mexicanas. Un análisis cualitativo

    Directory of Open Access Journals (Sweden)

    Ana María Chávez Hernández

    2011-01-01

    Full Text Available Objetivo. El análisis de notas suicidas es uno de los métodos más fructíferos en el estudio del comportamiento suicida. Bajo esta premisa, el objetivo de este trabajo fue describir y analizar las características afectivas, emocionales, cognoscitivas y actitudinales, relacionadas con el momento en que las personas planean su auto-destrucción, y que fueron expresadas en sus notas suicidas. Método. Estudio de tipo ex post facto, con una muestra de 142 notas suicidas que dejaron personas que se suicidaron (2005-2008 en el Estado de Guanajuato, México. Se realizó un análisis de contenido con el método de Inter-juez (0.96 coeficiente de concordancia; así como tablas de contingencia y escalamiento multidimensional. Resultados. Se muestra que el grupo de edad, entre 15 y 29 años, representan la mitad de los sujetos que consumaron el acto de suicidio (50% y que dejaron una nota póstuma. Se encontraron 11 categorías con diferencias estadísticamente significativas para lo cual se utilizó un escalamiento multidimensional. Los problemas de relaciones interpersonales fue la razón más mencionada para cometer suicidio (45.1% tanto en hombres como mujeres (X2 c = 6.856, p = 0.009. El 97.2% de los casos mostró curso lógico de pensamiento y consciencia de su actividad cognitiva y el 83% adecuada orientación en la realidad. Conclusión. Los resultados concuerdan con los arrojados por otros estudios internacionales con relación a la comprensión etiológica psicosocial del suicidio. Las razones manifiestas para el suicidio difieren según los grupos de edad, lo cual deberá considerarse en los dispositivos de detección y tratamiento de las personas con riesgo suicida.

  14. Alterações leucocitárias como factor de prognóstico na evolução clínica da parvovirose canina: 191 casos

    OpenAIRE

    Frazão, Pedro Saraiva Gonçalves dos Santos

    2008-01-01

    Dissertação de Mestrado Integrado em Medicina Veterinária O objectivo deste trabalho foi o de caracterizar a frequência da raça, sexo e idade, assim como a sazonalidade na parvovirose canina, e investigar se existe alguma associação entre o número total de leucócitos, o leucograma e o prognóstico da parvovirose canina (alta vs. óbito). Num estudo retrospectivo observacional, em 191 canídeos com diagnóstico clínico de parvovirose canina confirmado através de teste ELISA, obte...

  15. Immunity and immunosuppression in experimental visceral leishmaniasis

    Directory of Open Access Journals (Sweden)

    Goto H.

    2004-01-01

    Full Text Available Leishmaniasis is a disease caused by protozoa of the genus Leishmania, and visceral leishmaniasis is a form in which the inner organs are affected. Since knowledge about immunity in experimental visceral leishmaniasis is poor, we present here a review on immunity and immunosuppression in experimental visceral leishmaniasis in mouse and hamster models. We show the complexity of the mechanisms involved and differences when compared with the cutaneous form of leishmaniasis. Resistance in visceral leishmaniasis involves both CD4+ and CD8+ T cells, and interleukin (IL-2, interferon (IFN- gamma, and IL-12, the latter in a mechanism independent of IFN- gamma and linked to transforming growth factor (TGF-ß production. Susceptibility involves IL-10 but not IL-4, and B cells. In immune animals, upon re-infection, the elements involved in resistance are different, i.e., CD8+ T cells and IL-2. Since one of the immunopathological consequences of active visceral leishmaniasis in humans is suppression of T-cell responses, many studies have been conducted using experimental models. Immunosuppression is mainly Leishmania antigen specific, and T cells, Th2 cells and adherent antigen-presenting cells have been shown to be involved. Interactions of the co-stimulatory molecule family B7-CTLA-4 leading to increased level of TGF-ß as well as apoptosis of CD4+ T cells and inhibition of macrophage apoptosis by Leishmania infection are other components participating in immunosuppression. A better understanding of this complex immune response and the mechanisms of immunosuppression in experimental visceral leishmaniasis will contribute to the study of human disease and to vaccine development.

  16. Mielopatía Degenerativa canina: signos clínicos, diagnóstico y terapéutica (Canine degenerative myelopathy: clinical signs, diagnosis and therapy)

    OpenAIRE

    Suraniti, A.P; LR Mira, G; Fidanza, M; Guerrero, J.; Marina, ML; M. Mercado; Gilardoni, LR; Mundo, S.

    2011-01-01

    ResumenLa mielopatía degenerativa canina es una enfermedad neurológica progresiva autoinmune que afecta principalmente a caninos adultos de talla grande sin predilección de sexo.SummaryCanine degenerative myelopathy is a progressive neurological autoimmune disease that primarily affects large size adult dogs and no sex predilection.

  17. Effect of DNA extraction in the Rosa canina L. identification under different processing temperature

    Directory of Open Access Journals (Sweden)

    Jana Žiarovská

    2017-01-01

    Full Text Available Rosa canina, L. is widely used for medicinal purposes as well as in food industry where it is a valuable source, bioactive compounds and natural colorants. Actually, no specific method is recommended as suitable one for DNA extraction from rose hips. The aim of the study was to compare three commercial and three non-commercial methods to extract total genomic DNA from rose hips hyphanthium. Four methods are based on the precipitation in principle and two methods are based on resin-binding. Extracted DNA was proved for the effectivity in following PCR. In total, six different DNA isolations was performed for differently heat processes rose hips - fresh hyphanthium, 2-weeks frozen hyphanthium, dried hyphanthium (50 °C and boiled hyphanthium (100 °C. The amplification parameters of 500 bp chloroplast gene amplicon were evaluated. Obtained amounts of extracted DNA was very variable not only for every individual method used but for individual treatment of samples, too. In general, non-commercial method provided higher amount of extracted DNA, but the A260/280 ratio was lower. When regarding the processing treatment of the samples, high differences were found among the samples untreated by heat and those that were dried or boiled for three of the used extraction methods. All the samples were positive for amplification, but different amounts of amplified product were obtained. The comparison of data for concentrations of extracted DNA and concentrations of amplified product showed large differences when regarding the achieved purity of DNA in extraction.

  18. ESTUDIO SEROLÓGICO DE BRUCELOSIS CANINA EN DOS ALBERGUES DEL MUNICIPIO DE ENVIGADO, COLOMBIA (2011

    Directory of Open Access Journals (Sweden)

    P. Agudelo

    2014-01-01

    Full Text Available El objetivo de este estudio fue buscar la presencia de anticuerpos de brucelosis en la población de dos albergues caninos del municipio de Envigado (Antioquia, Colombia y a tal fin se seleccionaron 54 perros. Se tomaron muestras sanguíneas en 10 machos y 44 hembras que fueron procesadas bajo la técnica de inmunocromatografía para Brucella canis. No hubo diferencia estadística entre albergues en los parámetros ‘sexo’ y ‘raza’ de cada grupo de estudio y tampoco se encontró evidencia serológica de la presencia de brucelosis canina en el grupo de animales muestreados. Los resultados indican que, aunque los animales permanecieron en calidad de abandono durante un período de su vida, su condición feral no era permanente debido a que en el momento del estudio se encontraban en albergues. Las políticas intensivas de recolección se orientan más a la atención de mascotas abandonadas que a aquellos animales ferales (los cuales, con mayor frecuencia, pueden ser portadores de la enfermedad. El tipo de población in - cluida en el estudio, sumado a la práctica estricta de esterilización inmediata dentro del albergue —que a su vez impide los apareamientos entre individuos—, pueden incidir en la reducción la propagación de la enfermedad en el municipio.

  19. Efecto del sacrificio de perros vagabundos en el control de la rabia canina

    Directory of Open Access Journals (Sweden)

    Ricardo Castillo-Neyra

    Full Text Available En la ciudad de Arequipa, Perú, se han detectado perros con rabia desde marzo de 2015, señal de la reintroducción del virus de la rabia (VR en esta zona previamente declarada libre de esta enfermedad. La alta densidad de perros vagabundos ha sido considerada una de las causas de la continua transmisión del VR en el brote de la ciudad de Arequipa, alentando el sacrificio de perros como medida de salud pública. Sin embargo, la efectividad de esta medida de control no ha sido evaluada. El objetivo de esta revisión fue determinar la efectividad del sacrificio de perros vagabundos como medida de control de la rabia urbana. Se realizó la búsqueda de artículos sobre control de rabia canina o urbana en las bases de datos de Medline, Scopus y LILACS. Se incluyeron estudios originales y revisiones que hayan evaluado algún efecto del sacrificio de perros en la transmisión del VR. Veinte de 21 artículos concluyen que el sacrificio de perros vagabundos es inefectivo en reducir la trasmisión del VR e, incluso, puede tener consecuencias imprevistas que empeoren el problema. En conclusión, la evidencia disponible indica que sacrificar perros vagabundos no es efectivo para controlar la rabia urbana. Además, las diversas consecuencias negativas del sacrificio de los perros vagabundos reportadas en otras partes del mundo sugieren que el sistema de rabia urbana es altamente complejo y todos sus componentes deben ser tomados en cuenta durante la implementación de actividades de control.

  20. {sup 68}Ga-labeled NOTA-RGD-BBN peptide for dual integrin and GRPR-targeted tumor imaging

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Zhaofei [Stanford University School of Medicine, The Molecular Imaging Program at Stanford (MIPS), Department of Radiology and Bio-X Program, Stanford, CA (United States); Peking University, Medical Isotopes Research Center, Beijing (China); Niu, Gang; Chen, Xiaoyuan [Stanford University School of Medicine, The Molecular Imaging Program at Stanford (MIPS), Department of Radiology and Bio-X Program, Stanford, CA (United States); Wang, Fan [Peking University, Medical Isotopes Research Center, Beijing (China)

    2009-09-15

    Radiolabeled Arg-Gly-Asp (RGD) and bombesin (BBN) peptide analogs have been extensively investigated for the imaging of tumor integrin {alpha}{sub v}{beta}{sub 3} and gastrin-releasing peptide receptor (GRPR) expression, respectively. Recently, we designed and synthesized a RGD-BBN heterodimeric peptide from c(RGDyK) and BBN(7-14) through a glutamate linker. The goal of this study was to investigate the dual receptor-targeting property and tumor diagnostic value of RGD-BBN heterodimeric peptide labeled with generator-eluted {sup 68}Ga (t{sub 1/2} 68 min, {beta}{sup +} 89% and EC 11%), {sup 68}Ga-NOTA-RGD-BBN. RGD-BBN heterodimer was conjugated with 1,4,7-triazacyclononanetriacetic acid (NOTA) and labeled with {sup 68}Ga. The dual receptor binding affinity was investigated by a radioligand competition binding assay. The in vitro and in vivo dual receptor targeting of {sup 68}Ga-NOTA-RGD-BBN was evaluated and compared with that of {sup 68}Ga-NOTA-RGD and {sup 68}Ga-NOTA-BBN. NOTA-RGD-BBN had integrin {alpha}{sub v}{beta}{sub 3} and GRPR binding affinities comparable to those of the monomeric RGD and BBN, respectively. The dual receptor targeting property of {sup 68}Ga-NOTA-RGD-BBN was validated by blocking studies in a PC-3 tumor model. {sup 68}Ga-NOTA-RGD-BBN showed higher tumor uptake than {sup 68}Ga-NOTA-RGD and {sup 68}Ga-NOTA-BBN. {sup 68}Ga-NOTA-RGD-BBN can also image tumors with either integrin or GRPR expression. {sup 68}Ga-NOTA-RGD-BBN exhibited dual receptor targeting properties both in vitro and in vivo. The favorable characterizations of {sup 68}Ga-NOTA-RGD-BBN such as convenient synthesis, high specific activity, and high tumor uptake, warrant its further investigation for clinical cancer imaging. (orig.)

  1. Fungal lectin of Peltigera canina induces chemotropism of compatible Nostoc cells by constriction-relaxation pulses of cyanobiont cytoskeleton.

    Science.gov (United States)

    Díaz, Eva Maria; Vicente-Manzanares, Miguel; Sacristan, Mara; Vicente, Carlos; Legaz, Maria-Estrella

    2011-10-01

    A glycosylated arginase acting as a fungal lectin from Peltigera canina is able to produce recruitment of cyanobiont Nostoc cells and their adhesion to the hyphal surface. This implies that the cyanobiont would develop organelles to motility towards the chemoattractant. However when visualized by transmission electron microscopy, Nostoc cells recently isolated from P. canina thallus do not reveal any motile, superficial organelles, although their surface was covered by small spindles and serrated layer related to gliding. The use of S-(3,4-dichlorobenzyl)isothiourea, blebbistatin, phalloidin and latrunculin A provide circumstantial evidence that actin microfilaments rather than MreB, the actin-like protein from prokaryota, and, probably, an ATPase which develops contractile function similar to that of myosin II, are involved in cell motility. These experimental facts, the absence of superficial elements (fimbriae, pili or flagellum) related to cell movement, and the appearance of sunken cells during of after movement verified by scanning electron microscopy, support the hypothesis that the motility of lichen cyanobionts could be achieved by contraction-relaxation episodes of the cytoskeleton induced by fungal lectin act as a chemoattractant.

  2. Neuroprotective Effects of Herbal Extract (Rosa canina, Tanacetum vulgare and Urtica dioica) on Rat Model of Sporadic Alzheimer's Disease.

    Science.gov (United States)

    Daneshmand, Parvaneh; Saliminejad, Kioomars; Dehghan Shasaltaneh, Marzieh; Kamali, Koorosh; Riazi, Gholam Hossein; Nazari, Reza; Azimzadeh, Pedram; Khorram Khorshid, Hamid Reza

    2016-01-01

    Sporadic Alzheimer's Disease (SAD) is caused by genetic risk factors, aging and oxidative stresses. The herbal extract of Rosa canina (R. canina), Tanacetum vulgare (T. vulgare) and Urtica dioica (U. dioica) has a beneficial role in aging, as an anti-inflammatory and anti-oxidative agent. In this study, the neuroprotective effects of this herbal extract in the rat model of SAD was investigated. The rats were divided into control, sham, model, herbal extract -treated and ethanol-treated groups. Drug interventions were started on the 21(st) day after modeling and each treatment group was given the drugs by intraperitoneal (I.P.) route for 21 days. The expression levels of the five important genes for pathogenesis of SAD including Syp, Psen1, Mapk3, Map2 and Tnf-α were measured by qPCR between the hippocampi of SAD model which were treated by this herbal extract and control groups. The Morris Water Maze was adapted to test spatial learning and memory ability of the rats. Treatment of the rat model of SAD with herbal extract induced a significant change in expression of Syp (p=0.001) and Psen1 (p=0.029). In Morris Water Maze, significant changes in spatial learning seen in the rat model group were improved in herbal-treated group. This herbal extract could have anti-dementia properties and improve spatial learning and memory in SAD rat model.

  3. Colorectal visceral perception in diverticular disease

    NARCIS (Netherlands)

    Clemens, C. H. M.; Samsom, M.; Roelofs, J.; van Berge Henegouwen, G. P.; Smout, A. J. P. M.

    2004-01-01

    BACKGROUND AND AIMS: The pathogenesis of asymptomatic diverticular disease (ADD) and symptomatic uncomplicated diverticular disease (SUDD) has not been elucidated. The aim of our study was to assess whether altered visceral perception or abnormal compliance of the colorectal wall play a role in

  4. Sympathetic blocks for visceral cancer pain management

    DEFF Research Database (Denmark)

    Mercadante, Sebastiano; Klepstad, Pal; Kurita, Geana Paula

    2015-01-01

    The neurolytic blocks of sympathetic pathways, including celiac plexus block (CPB) and superior hypogastric plexus block (SHPB) , have been used for years. The aim of this review was to assess the evidence to support the performance of sympathetic blocks in cancer patients with abdominal visceral...

  5. Visceral Leishmaniasis in Rural Bihar, India

    Science.gov (United States)

    Singh, Shri Prakash; Malaviya, Paritosh; Picado, Albert; Gidwani, Kamlesh; Singh, Rudra Pratap; Menten, Joris; Boelaert, Marleen; Sundar, Shyam

    2012-01-01

    To identify factors associated with incidence of visceral leishmaniasis (VL), we surveyed 13,416 households in Bihar State, India. VL was associated with socioeconomic status, type of housing, and belonging to the Musahar caste. Annual coverage of indoor residual insecticide spraying was 12%. Increasing such spraying can greatly contribute to VL control. PMID:23017164

  6. Biased Intensity Judgements of Visceral Sensations After Learning to Fear Visceral Stimuli: A Drift Diffusion Approach.

    Science.gov (United States)

    Zaman, Jonas; Madden, Victoria J; Iven, Julie; Wiech, Katja; Weltens, Nathalie; Ly, Huynh Giao; Vlaeyen, Johan W S; Van Oudenhove, Lukas; Van Diest, Ilse

    2017-10-01

    A growing body of research has identified fear of visceral sensations as a potential mechanism in the development and maintenance of visceral pain disorders. However, the extent to which such learned fear affects visceroception remains unclear. To address this question, we used a differential fear conditioning paradigm with nonpainful esophageal balloon distensions of 2 different intensities as conditioning stimuli (CSs). The experiment comprised of preacquisition, acquisition, and postacquisition phases during which participants categorized the CSs with respect to their intensity. The CS+ was always followed by a painful electrical stimulus (unconditioned stimulus) during the acquisition phase and in 60% of the trials during postacquisition. The second stimulus (CS-) was never associated with pain. Analyses of galvanic skin and startle eyeblink responses as physiological markers of successful conditioning showed increased fear responses to the CS+ compared with the CS-, but only in the group with the low-intensity stimulus as CS+. Computational modeling of response times and response accuracies revealed that differential fear learning affected perceptual decision-making about the intensities of visceral sensations such that sensations were more likely to be categorized as more intense. These results suggest that associative learning might indeed contribute to visceral hypersensitivity in functional gastrointestinal disorders. This study shows that associative fear learning biases intensity judgements of visceral sensations toward perceiving such sensations as more intense. Learning-induced alterations in visceroception might therefore contribute to the development or maintenance of visceral pain. Copyright © 2017 American Pain Society. Published by Elsevier Inc. All rights reserved.

  7. Pathophysiology of human visceral obesity: an update.

    Science.gov (United States)

    Tchernof, André; Després, Jean-Pierre

    2013-01-01

    Excess intra-abdominal adipose tissue accumulation, often termed visceral obesity, is part of a phenotype including dysfunctional subcutaneous adipose tissue expansion and ectopic triglyceride storage closely related to clustering cardiometabolic risk factors. Hypertriglyceridemia; increased free fatty acid availability; adipose tissue release of proinflammatory cytokines; liver insulin resistance and inflammation; increased liver VLDL synthesis and secretion; reduced clearance of triglyceride-rich lipoproteins; presence of small, dense LDL particles; and reduced HDL cholesterol levels are among the many metabolic alterations closely related to this condition. Age, gender, genetics, and ethnicity are broad etiological factors contributing to variation in visceral adipose tissue accumulation. Specific mechanisms responsible for proportionally increased visceral fat storage when facing positive energy balance and weight gain may involve sex hormones, local cortisol production in abdominal adipose tissues, endocannabinoids, growth hormone, and dietary fructose. Physiological characteristics of abdominal adipose tissues such as adipocyte size and number, lipolytic responsiveness, lipid storage capacity, and inflammatory cytokine production are significant correlates and even possible determinants of the increased cardiometabolic risk associated with visceral obesity. Thiazolidinediones, estrogen replacement in postmenopausal women, and testosterone replacement in androgen-deficient men have been shown to favorably modulate body fat distribution and cardiometabolic risk to various degrees. However, some of these therapies must now be considered in the context of their serious side effects. Lifestyle interventions leading to weight loss generally induce preferential mobilization of visceral fat. In clinical practice, measuring waist circumference in addition to the body mass index could be helpful for the identification and management of a subgroup of overweight or obese

  8. Determinants for the development of visceral leishmaniasis disease.

    Directory of Open Access Journals (Sweden)

    Laura-Isobel McCall

    2013-01-01

    Full Text Available Leishmaniasis is a vector-borne neglected tropical disease associated with a spectrum of clinical manifestations, ranging from self-healing cutaneous lesions to fatal visceral infections. Among the most important questions in Leishmania research is why some species like L. donovani infect visceral organs, whereas other species like L. major remain in the skin. The determinants of visceral leishmaniasis are still poorly understood, although genomic, immunologic, and animal models are beginning to provide important insight into this disease. In this review, we discuss the vector, host, and pathogen factors that mediate the development of visceral leishmaniasis. We examine the progression of the parasite from the initial site of sand fly bite to the visceral organs and its ability to survive there. The identification of visceral disease determinants is required to understand disease evolution, to understand visceral organ survival mechanisms, and potentially to develop better interventions for this largely neglected disease.

  9. Thidiazuron Triggers Morphogenesis in Rosa canina L. Protocorm-like bodies by Changing Incipient Cell Fate

    Directory of Open Access Journals (Sweden)

    Kou eYaping

    2016-05-01

    Full Text Available Thidiazuron (N-phenyl-N'-1,2,3-thiadiazol-5-ylurea; TDZ is an artificial plant growth regulator that is widely used in plant tissue culture. Protocorm-like bodies (PLBs induced by TDZ serve as an efficient and rapid in vitro regeneration system in Rosa species. Despite this, the mechanism of PLB induction remains relatively unclear. TDZ, which can affect the level of endogenous auxins and cytokinins, converts the cell fate of rhizoid tips and triggers PLB formation and plantlet regeneration in Rosa canina L. In callus-rhizoids, which are rhizoids that co-develop from callus, auxin and a Z-type cytokinin accumulated after applying TDZ, and transcription of the auxin transporter gene RcPIN1 was repressed. The expression of RcARF4, RcRR1, RcCKX2, RcCKX3, and RcLOG1 increased in callus-rhizoids and rhizoid tips while the transcription of an auxin response factor (RcARF1 and auxin transport proteins (RcPIN2, RcPIN3 decreased in callus-rhizoids but increased in rhizoid tips. In situ hybridization of rhizoids showed that RcWUS and RcSERK1 were highly expressed in columella cells and root stem cells resulting in the conversion of cell fate into shoot apical meristems or embryogenic callus. In addition, transgenic XVE::RcWUS lines showed repressed RcWUS overexpression while RcWUS had no effect on PLB morphogenesis. Furthermore, higher expression of the root stem cell marker RcWOX5 and root stem cell maintenance regulator genes RcPLT1 and RcPLT2 indicated the presence of a dedifferentiation developmental pathway in the stem cell niche of rhizoids. Viewed together, our results indicate that different cells in rhizoid tips acquired regeneration competence after induction by TDZ. A novel developmental pathway containing different cell types during PLB formation was identified by analyzing the endogenous auxin and cytokinin content. This study also provides a deeper understanding of the mechanisms underlying in vitro regeneration in Rosa.

  10. Mordedura canina e atendimento antirrábico humano em Minas Gerais

    Directory of Open Access Journals (Sweden)

    V.M.R. Oliveira

    2012-08-01

    Full Text Available O objetivo do presente trabalho foi descrever e avaliar a mordedura canina e o atendimento antirrábico humano em Minas Gerais, de 1999 a 2004, correlacionando fontes de informação e áreas de risco predeterminadas para raiva humana transmitida por cão. Realizou-se um estudo observacional descritivo retrospectivo, utilizando-se, de forma adaptada, a análise exploratória de prontuários dos atendimentos da Superintendência de Epidemiologia da Secretaria de Estado da Saúde de Minas Gerais (339.012 de atendimentos, do Sistema de Informação de Notificação de Agravos, do Sistema de Informações sobre Mortalidade, do Sistema de Informações Hospitalares e do Programa Nacional de Imunizações (132.452 fichas. Para a classificação dos agravos, usou-se o Código Internacional de Doenças (10ª revisão. Os dados foram armazenados e analisados com auxílio dos softwares Epi-Info, Tab-Win e Office®. Verificou-se que o tratamento antirrábico humano é excessivo nas áreas de baixo e médio risco para raiva e, ao contrário, reduzido nas áreas de alto risco. O perfil do paciente é estudante masculino, menor de 14 anos, residente em área urbana de baixo risco para raiva humana transmitida por cão, com mordedura única nos membros, provocada por cão sadio e observável. Os sistemas de informação não oferecem a confiabilidade necessária ao médico responsável para a prescrição do tratamento antirrábico adequado. A profilaxia da raiva deve ter um aspecto multicêntrico, com interfaces na atenção tanto à saúde humana quanto à animal, o que não tem ocorrido, propiciando falhas na vigilância e no atendimento do agravo.

  11. Contaminación fecal canina en plazas y veredas de Buenos Aires, 1991-2006

    Directory of Open Access Journals (Sweden)

    Diana Rubel

    2010-08-01

    Full Text Available El objetivo del trabajo fue presentar y analizar los datos de contaminación fecal canina y parasitaria en plazas y veredas de Buenos Aires obtenidos entre 1991 y 2006 por la Cátedra Parasitología General (Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires. Se censaron las heces en varias plazas cada año en un único día entre 1991-2006. A partir del año 2000 se censaron heces en veredas circundantes. En cada plaza se seleccionaron al azar 30 heces frescas que se conservaron en formol 5% para su análisis parasitológico por el método de flotación de Willis con solución saturada de ClNa. Los 51 censos presentaron una mediana de heces de 288; un 82% de las heces se observaron en los canteros de tierra o pasto. La contaminación fecal en las plazas fue mayor en el período 2000-06 que en el anterior (p = 0.0000. La contaminación fecal de las plazas aumentó con la densidad humana en las manzanas lindantes (p = 0.0076. Las veredas mostraron un patrón inverso, ya que la contaminación fecal fue mayor en las áreas menos densamente pobladas (p = 0.0000. Se detectaron parásitos en un número variable de las heces colectadas en todas las plazas. Los géneros más frecuentes fueron Ancylostoma (20.47%, Trichuris (2.59% y Toxocara (1.70%. Nuestros resultados indican un aumento en la contaminación fecal de las plazas, posiblemente asociado con el aumento del número de perros en la ciudad combinado con las deficiencias en la implementación y el seguimiento de las medidas de control.

  12. Thidiazuron Triggers Morphogenesis in Rosa canina L. Protocorm-Like Bodies by Changing Incipient Cell Fate.

    Science.gov (United States)

    Kou, Yaping; Yuan, Cunquan; Zhao, Qingcui; Liu, Guoqin; Nie, Jing; Ma, Zhimin; Cheng, Chenxia; Teixeira da Silva, Jaime A; Zhao, Liangjun

    2016-01-01

    Thidiazuron (N-phenyl-N'-1,2,3-thiadiazol-5-ylurea; TDZ) is an artificial plant growth regulator that is widely used in plant tissue culture. Protocorm-like bodies (PLBs) induced by TDZ serve as an efficient and rapid in vitro regeneration system in Rosa species. Despite this, the mechanism of PLB induction remains relatively unclear. TDZ, which can affect the level of endogenous auxins and cytokinins, converts the cell fate of rhizoid tips and triggers PLB formation and plantlet regeneration in Rosa canina L. In callus-rhizoids, which are rhizoids that co-develop from callus, auxin and a Z-type cytokinin accumulated after applying TDZ, and transcription of the auxin transporter gene RcPIN1 was repressed. The expression of RcARF4, RcRR1, RcCKX2, RcCKX3, and RcLOG1 increased in callus-rhizoids and rhizoid tips while the transcription of an auxin response factor (RcARF1) and auxin transport proteins (RcPIN2, RcPIN3) decreased in callus-rhizoids but increased in rhizoid tips. In situ hybridization of rhizoids showed that RcWUS and RcSERK1 were highly expressed in columella cells and root stem cells resulting in the conversion of cell fate into shoot apical meristems or embryogenic callus. In addition, transgenic XVE::RcWUS lines showed repressed RcWUS overexpression while RcWUS had no effect on PLB morphogenesis. Furthermore, higher expression of the root stem cell marker RcWOX5 and root stem cell maintenance regulator genes RcPLT1 and RcPLT2 indicated the presence of a dedifferentiation developmental pathway in the stem cell niche of rhizoids. Viewed together, our results indicate that different cells in rhizoid tips acquired regeneration competence after induction by TDZ. A novel developmental pathway containing different cell types during PLB formation was identified by analyzing the endogenous auxin and cytokinin content. This study also provides a deeper understanding of the mechanisms underlying in vitro regeneration in Rosa.

  13. DETECCIÓN DE ANTICUERPOS ANTI-Leishmania (Trypanosomatidae EN POBLACIONES CANINAS DEL DEPARTAMENTO DE SUCRE, COLOMBIA

    Directory of Open Access Journals (Sweden)

    Luís Enrique Paternina Tuirán

    2015-11-01

    Full Text Available RESUMENEl objetivo de la investigación fue determinar la prevalencia de anticuerpos anti-Leishmania en perros del departamento de Sucre, Colombia. Se analizaron 122 perros del área rural de los municipios de Ovejas, Sampués y Sincelejo, mediante la técnica de inmunofluorescencia indirecta (IFI. Anticuerpos anti-Leishmania fueron detectados en el 69,6 % (IC95 % = 61,1 – 78,2 de la población canina estudiada. En el municipio de Sincelejo la seropositividad fue del 72 % (IC95 % = 61,1 – 82,8, en el municipio de Sampués del 68,4 % (IC95 % = 52,3 – 84,5 y en el municipio de Ovejas del 55,5 % (IC95 % = 21,2 – 86,3. Los animales seropositivos fueron principalmente caninos jóvenes y adultos. El 17,6 % de los perros seropositivos mostró signos clínicos compatibles con leishmaniasis canina, entre los cuales la onicogrifosis, alopecia y caquexia fueron los más comunes. La alta frecuencia de caninos con anticuerpos a Leishmania detectada en el presente estudio, pone de manifiesto la hiperendemicidad de la leishmaniasis canina en esta zona del país, así como el alto riesgo de brotes epidémicos de la enfermedad.ABSTRACTThe aim of this survey was to determine prevalence of antibodies against Leishmania in dogs from Sucre, Colombia. We analyzed 122 dogs in rural areas from municipalities of Sampués, Ovejas and Sincelejo, by indirect immunofluorescence (IFA. Anti-Leishmania antibodies were detected in 69.6 % (95 % CI = 61.1 to 78.2 of the selected canine population. In the municipality of Sincelejo, Sampues and Ovejas, the seropositivities were 72 % (95 % CI = 61.1 to 82.8, 68.4 % (95 % CI = 52.3 to 84.5 and 55.5 % (95 % CI = 21.2 to 86.3 respectively. Seropositive animals were mainly young and adult dogs. The 17.6 % of seropositive dogs showed clinical signs compatible with canine leishmaniasis; onychogryphosis, alopecia and cachexia were the most common ones. The detected high frequency of canines with antibodies against Leishmania in the

  14. Notas para uma pronúncia do francês

    OpenAIRE

    César Reis

    2011-01-01

    Resumo: Propomos, neste artigo, elementos para discussão a respeito da questão do nível de pronúncia que se deve exigir dos estudantes brasileiros do francês. Denominamos “pronúncia aceitável” aquela que distingue os fonemas da língua, que não incorpora transferências dos processos fonológicos mais produtivos do português e, finalmente, que não apresenta características individuais marcantes. Apresentamos, também, algumas notas sobre a pronúncia do francês.Palavras-chave: Língua francesa; fon...

  15. Notas sobre el género Pithecellobium Martius

    Directory of Open Access Journals (Sweden)

    Barbosa César

    1986-12-01

    Full Text Available La presente nota se realizó como resultado parcial de la revisión que se adelantó sobre la Sección Caulanthon Bentham del género Pithecellobium en Colombia.  MOHLEMBROCK(1963, en un intento por organizar las categorías subgenéricas de Pithecellobium dio un paso de singular importancia al reunir algunos de los segregados genéricos de BRITTON &. ROSE (1928, BRITTON &. KILLIP (1936 Y KOSTERMANS (1954 entre otros; éstos, como oportunamente lo anotó  NIELSEN  (1981, son géneros basados en una sola característica diferencial, tal es el caso de Punjuba que se trató de separar  por presentar s ernillas exaridadas e inflorescencias en grandes racimos o espigas, caracteres que son válidos para separar series de especies.

  16. Bioactive ingredients of rose hips (Rosa canina L with special reference to antioxidative and anti-inflammatory properties: in vitro studies

    Directory of Open Access Journals (Sweden)

    Winther K

    2016-02-01

    Full Text Available Kaj Winther,1 Anne Sophie Vinther Hansen,1 Joan Campbell-Tofte2 1Department of Nutrition, Exercise and Sports, University of Copenhagen, Frederiksberg C, Denmark; 2Coordinating Research Unit, Frederiksberg University Hospital, Copenhagen, Denmark Abstract: Rosa canina pseudo fruits, often referred to as rose hips, have been used as herbal medicine for more than 2,000 years, yet research has only recently begun to clarify specific mechanisms by which this plant product affects human health. Numerous compounds have been identified, and speculations of their bioactivity have implicated flavonoids, carotenoids, and fatty acids (FAs. With more than 4,500 representatives, flavonoids have been subjected to comprehensive research, with results that suggest various individual structures may be health-promoting compounds, also in rose hips. The importance of carotenoids from R. canina is currently being debated, because the demonstration of specific bioactivity among this group is presently less clear. The benefits of specific FAs have been investigated for decades, and several types of FAs are termed “essential” for human health. The specific mechanisms for bioactivity associated with three FAs that are abundant in R. canina fruits have been clarified in research. For example, linoleic acid, α-linolenic acid (mostly present in the seeds from R. canina and a galactolipid ((2S-1,2-di-O-[(9Z,12Z,15Z-octadeca-9-12-15-trienoyl]-3-O-β-d-galactopyranosyl glycerol, referred to as GOPO, have been shown to have anti-inflammatory properties. The aim of this review is to critically analyze the published literature on rose hip research, with emphasis on the broadness and varying significance of the publications. Initially, we describe the chemical ingredients of R. canina pseudo fruits, with some focus on what ingredients are found in the whole pseudo fruit and what we know is confined to the seeds (achene seeds, and/or the shells (hypanthium. Then, we evaluate

  17. Acute type B aortic dissection complicated by visceral ischemia.

    Science.gov (United States)

    Jonker, Frederik H W; Patel, Himanshu J; Upchurch, Gilbert R; Williams, David M; Montgomery, Daniel G; Gleason, Thomas G; Braverman, Alan C; Sechtem, Udo; Fattori, Rossella; Di Eusanio, Marco; Evangelista, Arturo; Nienaber, Christoph A; Isselbacher, Eric M; Eagle, Kim A; Trimarchi, Santi

    2015-04-01

    Acute type B aortic dissection (ABAD) can lead to visceral malperfusion, a potentially life-threatening complication. The purpose of this study was to investigate the presentation, management, and outcomes of ABAD patients with visceral ischemia who are enrolled in the International Registry of Acute Aortic Dissection. Patients with ABAD enrolled in the registry between 1996 and 2013 were identified and stratified based on presence of visceral ischemia at admission. Demographics, medical history, imaging results, management, and outcomes were compared for patients with versus without visceral ischemia. A total of 1456 ABAD patients were identified, of which 104 (7.1%) presented with visceral ischemia. Preoperative limb ischemia (28% vs 7%, P < .001) and acute renal failure (41% vs 14%, P < .001) were more common among patients with visceral ischemia. Endovascular treatment and surgery were offered to 49% and 30% of the visceral ischemia cohort, respectively; remaining patients were managed conservatively. The in-hospital mortality was 30.8% for patients with visceral ischemia and 9.1% for those without visceral ischemia (odds ratio [OR] 4.44; 95% confidence interval [CI], 2.8-7.0, P < .0001). Mortality rates were similar after surgical and endovascular management of visceral ischemia (25.8% and 25.5%, respectively, P = not significant). Among the visceral ischemia group, medical management was a predictor of mortality in multivariate analysis (OR, 5.91; 95% CI, 1.2-31.0; P = .036). Patients with ABAD complicated by visceral ischemia have a high risk of mortality. We observed similar outcomes for patients treated by endovascular management versus surgery, whereas medical management was an independent predictor of mortality. Early diagnosis and intervention for visceral ischemia seems to be crucial. Copyright © 2015 The American Association for Thoracic Surgery. Published by Elsevier Inc. All rights reserved.

  18. Programa de control de la población canina en el distrito de Surquillo Lima, Perú

    OpenAIRE

    Arata Azcárate, Carla; Reategui Vargas, Gianina

    2016-01-01

    La falta de control en la población canina y el elevado número de perros sin propietario en las calles (denominados “perros callejeros”) es un problema que afecta a la gran mayoría de ciudades del mundo. La presencia de perros en la vía pública es una imagen común en Latinoamérica y en el Perú. Se estima que en el Perú hay más de 6 millones de perros viviendo en las calles. El 60% de las personas no creen que es importante hacerse responsables de sus mascotas porque siguen considerando qu...

  19. Characterization of biochemical traits of dog rose (Rosa canina L.) ecotypes in the central part of Iran.

    Science.gov (United States)

    Javanmard, Milad; Asadi-Gharneh, Hossein Ali; Nikneshan, Pejman

    2017-11-14

    Dog rose (Rosa canina L.) is a wild native species in Iran, with a significant genetic diversity. This plant serves as a rich source of vitamin C, anthocyanins, phenolic contents and carotenoids. Rose hips have been used in several food products, as well as perfumery and cosmetics industries. In this research, we investigate biochemical characteristics of five dog rose ecotypes (Kopehjamshid, Zarneh, Miyankish, Aghcheh and Sadeghiyeh), that were collected from the central part of Iran (Isfahan province). Amounts of vitamin C, total carotenoids, total phenolic contents, total anthocyanins, macro and micro minerals were measured. Seed oil are extracted by soxhlet method and analysed by gas chromatography. The macro and micro minerals levels in the fruit vary significantly among these regions. The results of this study demonstrate that dog rose have great diversity and can be used in breeding programmes in order to increase nutrient values as a food resource additive.

  20. Endemic transmission of visceral leishmaniasis in Bhutan.

    Science.gov (United States)

    Yangzom, Thinley; Cruz, Israel; Bern, Caryn; Argaw, Daniel; den Boer, Margriet; Vélez, Iván Dario; Bhattacharya, Sujit K; Molina, Ricardo; Alvar, Jorge

    2012-12-01

    Visceral leishmaniasis was first reported in Bhutan in 2006. We conducted studies of the parasite, possible vectors and reservoirs, and leishmanin skin test and risk factor surveys in three villages. Nineteen cases were reported from seven districts. Parasite typing yielded two novel microsatellite sequences, both related to Indian L. donovani. In one case village, 40 (18.5%) of 216 participants had positive leishmanin skin test results, compared with 3 (4.2%) of 72 in the other case village and 0 of 108 in the control village. Positive results were strongly associated with the village and increasing age. None of the tested dogs were infected. Eighteen sand flies were collected, 13 Phlebotomus species and 5 Sergentomyia species; polymerase chain reaction for leishmanial DNA was negative. This assessment suggests that endemic visceral leishmaniasis transmission has occurred in diverse locations in Bhutan. Surveillance, case investigations, and further parasite, vector, and reservoir studies are needed. The potential protective impact of bed nets should be evaluated.

  1. The Valjean effect: Visceral states and cheating.

    Science.gov (United States)

    Williams, Elanor F; Pizarro, David; Ariely, Dan; Weinberg, James D

    2016-09-01

    Visceral states like thirst, hunger, and fatigue can alter motivations, predictions, and even memory. Across 3 studies, we demonstrate that such "hot" states can also shift moral standards and increase dishonest behavior. Compared to participants who had just eaten or who had not yet exercised, hungry and thirsty participants were more likely to behave dishonestly to win a prize. Consistent with the specificity of motivation that is characteristic of visceral states, participants were only more likely to cheat for a prize that could alleviate their current deprived state (such as a bottle of water). Interestingly, this increase in dishonest behavior did not seem to be driven by an increase in the perceived monetary value of the prize. (PsycINFO Database Record (c) 2016 APA, all rights reserved).

  2. Immunity to visceral leishmaniasis: implications for immunotherapy.

    Science.gov (United States)

    Khadem, Forough; Uzonna, Jude E

    2014-01-01

    Visceral leishmaniasis, caused by Leishmania donovani, L. infantum (syn. Leishmania chagasi), is a globally widespread disease with a burden of about 400,000 new infections reported annually. It is the most dangerous form of human leishmaniasis in terms of mortality and morbidity and is spreading to several nonendemic areas because of migration, global traveling and military conflicts. The emergence of Leishmania-HIV co-infection and increased prevalence of drug-resistant strains have worsened the impact of the disease. The traditional low-cost drugs are often toxic with several adverse effects, highlighting the need for development of new therapeutic and prophylactic strategies. Therefore, a detailed understanding of mechanisms of protective immunity is extremely important in order to develop new therapeutics in the form of vaccines or immunotherapies. This review gives an overview of visceral leishmaniasis, with particular emphasis on the innate and adaptive immune responses, vaccine and vaccination strategies and their potentials for immunotherapy against the disease.

  3. Management of visceral leishmaniasis: Indian perspective

    OpenAIRE

    Agrawal S; Rai M; Sundar S

    2005-01-01

    Diagnosis and treatment of Indian visceral leishmaniasis (VL) is extremely unsatisfactory. For diagnosis, demonstration of parasites in splenic/marrow smears remains the gold standard, though k39 rapid strip test is a useful method in regions where access to parasite demonstration is difficult. pentavalent antimony remains the mainstay for the treatment of all forms of leishmaniasis globally; however, development of large-scale antimony resistance in Bihar has necessitated search for alternat...

  4. Vigilância de leishmaniose visceral americana em cães de área não endêmica, São Paulo

    Directory of Open Access Journals (Sweden)

    Savani Elisa San Martin Mouriz

    2003-01-01

    Full Text Available Realizou-se inquérito sorológico em cães domiciliados e errantes do Município de São José do Rio Preto, SP, para identificar animais infectados e detectar a possibilidade de transmissão da leishmaniose visceral americana. De novembro de 1998 a junho de 2000, 2.104 amostras de soros foram testadas por meio da reação de imunofluorescência indireta, empregando-se como antígeno formas promastigotas de Leishmania (L. chagasi. Observaram-se 2.092 amostras não reagentes e 12 reagentes. Dos cães com sorologia positiva foi possível realizar raspados de lesão em três animais. O material fixado em lâminas foi corado por Giemsa e, em apenas um, foram encontradas formas amastigotas características de Leishmania sp. Este resultado indica a necessidade de manutenção da vigilância sorológica canina e entomológica no município de São José do Rio Preto, a fim de detectar, precocemente, qualquer alteração na epidemiologia local.

  5. Vigilância de leishmaniose visceral americana em cães de área não endêmica, São Paulo

    Directory of Open Access Journals (Sweden)

    Elisa San Martin Mouriz Savani

    2003-04-01

    Full Text Available Realizou-se inquérito sorológico em cães domiciliados e errantes do Município de São José do Rio Preto, SP, para identificar animais infectados e detectar a possibilidade de transmissão da leishmaniose visceral americana. De novembro de 1998 a junho de 2000, 2.104 amostras de soros foram testadas por meio da reação de imunofluorescência indireta, empregando-se como antígeno formas promastigotas de Leishmania (L. chagasi. Observaram-se 2.092 amostras não reagentes e 12 reagentes. Dos cães com sorologia positiva foi possível realizar raspados de lesão em três animais. O material fixado em lâminas foi corado por Giemsa e, em apenas um, foram encontradas formas amastigotas características de Leishmania sp. Este resultado indica a necessidade de manutenção da vigilância sorológica canina e entomológica no município de São José do Rio Preto, a fim de detectar, precocemente, qualquer alteração na epidemiologia local.

  6. Antioxidant and cytotoxicitic effects of aqueous and alcoholic extracts of Rose Canina fruit on on the cell line U937

    Directory of Open Access Journals (Sweden)

    2015-12-01

    Full Text Available Background : Reactive oxygen species (ROS can be lethal for the cell. Edible plants due to containing antioxidants reduce risk of disease and damages resulting from ROS. The aim of this study was to determine the antioxidant and cytotoxicity effects of aqueous and alcoholic fruit extracts of Rose Canina on the cell line U937. Materials and Methods: Cell line U937 (cell leukemia , human purchased from the Pasteur Institute were incubated for 24 hours.Oxidative stress was created by iron ( 50 mM or copper ( 50 mM and H2O2 (mM1% for cells, and the selected antioxidants ( aqueous and alcoholic extract, 15 µmol of separate concentration levels of 25,50, and 100 mM were added to them (except for control samples. Glutathione levels were measured by Tietze. Lipid peroxidation was detected by MDA assay method. LDH kit was used for cytotoxicity assessments. Data were analyzed using ANOVA and Tukey’s test. Results: Mean and Sd of MDA for alcoholic extract was 0.6338 and 0.0012 µm/mg protein and for aqueous extract were 0.6389 and 0.0017 vs. for control: 0.6570 and 0.0023, respectively (p<0.05. There was no significant differentce between glutathione amounts among alcoholic and aqueous extract samples and control sample. Aqueous and alcoholic extracts 25% more than other concentrations significantly decreased the MDA. Alcoholic extract 100% had the most(62/15% and 25% had the lowest (24/1% toxicity. Conclusion: Alcholic and aqueous extracts of Rosa Canina fruit lead to decrease in lipid peroxidation. Their high concentrations have the most toxicity.

  7. Visceral adiposity, insulin resistance and cancer risk

    LENUS (Irish Health Repository)

    Donohoe, Claire L

    2011-06-22

    Abstract Background There is a well established link between obesity and cancer. Emerging research is characterising this relationship further and delineating the specific role of excess visceral adiposity, as opposed to simple obesity, in promoting tumorigenesis. This review summarises the evidence from an epidemiological and pathophysiological perspective. Methods Relevant medical literature was identified from searches of PubMed and references cited in appropriate articles identified. Selection of articles was based on peer review, journal and relevance. Results Numerous epidemiological studies consistently identify increased risk of developing carcinoma in the obese. Adipose tissue, particularly viscerally located fat, is metabolically active and exerts systemic endocrine effects. Putative pathophysiological mechanisms linking obesity and carcinogenesis include the paracrine effects of adipose tissue and systemic alterations associated with obesity. Systemic changes in the obese state include chronic inflammation and alterations in adipokines and sex steroids. Insulin and the insulin-like growth factor axis influence tumorigenesis and also have a complex relationship with adiposity. There is evidence to suggest that insulin and the IGF axis play an important role in mediating obesity associated malignancy. Conclusions There is much evidence to support a role for obesity in cancer progression, however further research is warranted to determine the specific effect of excess visceral adipose tissue on tumorigenesis. Investigation of the potential mechanisms underpinning the association, including the role of insulin and the IGF axis, will improve understanding of the obesity and cancer link and may uncover targets for intervention.

  8. Regulation of immunity during visceral Leishmania infection.

    Science.gov (United States)

    Rodrigues, Vasco; Cordeiro-da-Silva, Anabela; Laforge, Mireille; Silvestre, Ricardo; Estaquier, Jérôme

    2016-03-01

    Unicellular eukaryotes of the genus Leishmania are collectively responsible for a heterogeneous group of diseases known as leishmaniasis. The visceral form of leishmaniasis, caused by L. donovani or L. infantum, is a devastating condition, claiming 20,000 to 40,000 lives annually, with particular incidence in some of the poorest regions of the world. Immunity to Leishmania depends on the development of protective type I immune responses capable of activating infected phagocytes to kill intracellular amastigotes. However, despite the induction of protective responses, disease progresses due to a multitude of factors that impede an optimal response. These include the action of suppressive cytokines, exhaustion of specific T cells, loss of lymphoid tissue architecture and a defective humoral response. We will review how these responses are orchestrated during the course of infection, including both early and chronic stages, focusing on the spleen and the liver, which are the main target organs of visceral Leishmania in the host. A comprehensive understanding of the immune events that occur during visceral Leishmania infection is crucial for the implementation of immunotherapeutic approaches that complement the current anti-Leishmania chemotherapy and the development of effective vaccines to prevent disease.

  9. Visceral Afferent Pathways and Functional Brain Imaging

    Directory of Open Access Journals (Sweden)

    Stuart W.G. Derbyshire

    2003-01-01

    Full Text Available The application of functional imaging to study painful sensations has generated considerable interest regarding insight into brain dysfunction that may be responsible for functional pain such as that suffered in patients with irritable bowel syndrome (IBS. This review provides a brief introduction to the development of brain science as it relates to pain processing and a snapshot of recent functional imaging results with somatic and visceral pain. Particular emphasis is placed on current hypotheses regarding dysfunction of the brain-gut axis in IBS patients. There are clear and interpretable differences in brain activation following somatic as compared with visceral noxious sensation. Noxious visceral distension, particularly of the lower gastrointestinal tract, activates regions associated with unpleasant affect and autonomic responses. Noxious somatic sensation, in contrast, activates regions associated with cognition and skeletomotor responses. Differences between IBS patients and control subjects, however, were far less clear and interpretable. While this is in part due to the newness of this field, it also reflects weaknesses inherent within the current understanding of IBS. Future use of functional imaging to examine IBS and other functional disorders will be more likely to succeed by describing clear theoretical and clinical endpoints.

  10. The effects of rose hip (Rosa canina) on plasma antioxidative activity and C-reactive protein in patients with rheumatoid arthritis and normal controls

    DEFF Research Database (Denmark)

    Nielsen, Bente Kirkeskov; Christensen, Robin; Bügel, Susanne Gjedsted

    2011-01-01

    OBJECTIVES: Rose hip (Rosa canina) has been used as an herbal remedy against a wide range of ailments including inflammatory disorders. The anti-inflammatory and antioxidant properties of rose hips have been evaluated in vitro and active constituents have been isolated. Rose hip contains antioxid......OBJECTIVES: Rose hip (Rosa canina) has been used as an herbal remedy against a wide range of ailments including inflammatory disorders. The anti-inflammatory and antioxidant properties of rose hips have been evaluated in vitro and active constituents have been isolated. Rose hip contains...... reductase and catalase and the inflammatory marker C-reactive protein (CRP). The participants kept a food diary for the first 3 days and the last 3 days of the intervention period. The RA-patients completed The Stanford Health Assessment Questionnaire at baseline and follow-up. RESULTS: CRP...

  11. The role of rose hip (Rosa canina L) powder in alleviating arthritis pain and inflammation – part II animal and human studies

    OpenAIRE

    Marstrand, Kristian; Campbell-Tofte, Joan

    2016-01-01

    Kristian Marstrand,1 Joan Campbell-Tofte2 1Department of Orthopaedic Surgery, Elverum Hospital, Elverum, Norway; 2Coordinating Research Unit, Frederiksberg University Hospital, Frederiksberg, Copenhagen, Denmark Abstract: Rosa canina fruits (often known as rose hip) have been used in herbal remedies since ancient times. On the basis of anecdotal reports about the effects of rose hip preparations on human health and citations to the same in ancient texts, in vitro and in vivo studies have bee...

  12. The role of rose hip (Rosa canina L) powder in alleviating arthritis pain and inflammation – part II animal and human studies

    OpenAIRE

    Marstr; De, K.; Campbell-Tofte J

    2016-01-01

    Kristian Marstrand,1 Joan Campbell-Tofte2 1Department of Orthopaedic Surgery, Elverum Hospital, Elverum, Norway; 2Coordinating Research Unit, Frederiksberg University Hospital, Frederiksberg, Copenhagen, Denmark Abstract: Rosa canina fruits (often known as rose hip) have been used in herbal remedies since ancient times. On the basis of anecdotal reports about the effects of rose hip preparations on human health and citations to the same in ancient texts, in vitro and in vivo studies have bee...

  13. Extraction and Characterization of Phenolic Compounds from Rose Hip (Rosa canina L.) Using Liquid Chromatography Coupled with Electrospray Ionization - Mass Spectrometry

    OpenAIRE

    Andreea STĂNILĂ; Zoriţa DIACONEASA; Ioana ROMAN; Nicușor SIMA; Dănuț MĂNIUȚIU; Alin ROMAN; Rodica SIMA

    2015-01-01

    Wild berry are a rich of natural compounds which provide them high antioxidant potential. The compounds which provide them these proprieties are known to be vitamins, flavonoids, anthocyanins and phenolic acids. The aim of this study was to extract and characterize bioactive compounds from rose hip (Rosa canina L.) currently found in Romania. A qualitative high-performance liquid chromatography coupled with electrospray ionization mass spectrometric (ESI-MS) detection in positive ion mode has...

  14. AGLUTINACIÓN EN LÁTEX, ELISA Y HEMOAGLUTINACIÓN: ALTERNATIVAS PARA EL DIAGNÓSTICO DE LA PARVOVIROSIS CANINA EN HECES

    Directory of Open Access Journals (Sweden)

    Ramírez G. C.

    2005-12-01

    Full Text Available En vista de su alta morbilidad y letalidad, se han desarrollado varios métodos para el diagnóstico de la parvovirosis canina. Los más utilizados se basan en la detección de partículas virales excretadas en las heces durante la fase aguda de la enfermedad. Algunos requieren equipos especializados, lo que aumenta los costos y el tiempo de diagnóstico. Por esto, la simplificación de los métodos de diagnóstico con alta sensibilidad y especificidad es prioritaria. Este trabajo presenta el desarrollo de la técnica de aglutinación en látex para el diagnóstico de la parvovirosis canina en heces, comparada con un ELISA comercial y una hemoaglutinación. Sobre una población de 71 caninos con signos compatibles con parvovirosis canina, utilizando la prueba ELISA (SNAP® como referencia, se seleccionaron 21 caninos positivos. Empleando las muestras de materia fecal de los animales positivos se desarrollaron y compararon las pruebas de hemoaglutinación y aglutinación en látex. Se encontró una sensibili¬dad del 100% y una especificidad del 100% para aglutinación en látex y una sensibilidad del 95% y una especificidad del 100% para hemoaglutinación. Dada la sencillez de la prueba, los resultados obtenidos y sus costos, se recomienda la prueba de aglutinación en látex para el diagnóstico rápido y seguro de la parvovirosis canina.

  15. Sarcopenia and Visceral Obesity in Esophageal and Gastric Cancer

    Science.gov (United States)

    2017-02-17

    Esophageal Cancer; Gastric Cancer; Sarcopenia; Sarcopenic Obesity; Obesity; Visceral Obesity; Quality of Life; Surgery; Complication of Treatment; Chemotherapeutic Toxicity; Physical Activity; Oncology

  16. Apical constriction in distal visceral endoderm cells initiates global, collective cell rearrangement in embryonic visceral endoderm to form anterior visceral endoderm.

    Science.gov (United States)

    Shioi, Go; Hoshino, Hideharu; Abe, Takaya; Kiyonari, Hiroshi; Nakao, Kazuki; Meng, Wenxiang; Furuta, Yasuhide; Fujimori, Toshihiko; Aizawa, Shinichi

    2017-09-01

    The behavior of visceral endoderm cells was examined as the anterior visceral endoderm (AVE) formed from the distal visceral endoderm (DVE) using the mouse lines R26-H2B-EGFP and R26-PHA7-EGFP to visualize cell nuclei and adherens junction, respectively. The analysis using R26-H2B-EGFP demonstrated global cell rearrangement that was not specific to the DVE cells in the monolayer embryonic visceral endoderm sheet; each population of the endoderm cells moved collectively in a swirling movement as a whole. Most of the AVE cells at E6.5 were not E5.5 DVE cells but were E5.5 cells that were located caudally behind them, as previously reported (Hoshino et al., 2015; Takaoka et al., 2011). In the rearrangement, the posterior embryonic visceral endoderm cells did not move, as extraembryonic visceral endoderm cells did not, and they constituted a distinct population during the process of anterior-posterior axis formation. The analysis using R26-PHA7-EGFP suggested that constriction of the apical surfaces of the cells in prospective anterior portion of the DVE initiated the global cellular movement of the embryonic visceral endoderm to drive AVE formation. Copyright © 2017 Elsevier Inc. All rights reserved.

  17. Larva migrans visceral: relato de caso Visceral larva migrans: case report

    Directory of Open Access Journals (Sweden)

    Alexandre Bortoli Machado

    2003-04-01

    Full Text Available Larva migrans visceral é doença infecciosa, adquirida por ingestão de ovos provenientes dos vermes Toxocara canis e/ou Toxocara cati que infestam cães e gatos; as larvas penetram a parede intestinal e migram através dos tecidos levando a alterações diversas, conseqüentes a uma resposta inflamatória imune.¹ Os autores descrevem um caso clínico de larva migrans visceral com apresentação clínica atípica.Visceral larva migrans is an infectious human disease that occurs following ingestion of eggs from the environment originating from roundworms which commonly infect dogs and cats, Toxocara canis and Toxocara cati. The larvae penetrate the gut wall and migrate through the tissues causing disorders consequent to an inflammatory immune response¹. The authors describe a clinical case of visceral larva migrans with an unusual clinical presentation and also its clinical aspects, diagnosis and treatment are reviewed.

  18. Stress-Induced Chronic Visceral Pain of Gastrointestinal Origin

    Directory of Open Access Journals (Sweden)

    Beverley Greenwood-Van Meerveld

    2017-11-01

    Full Text Available Visceral pain is generally poorly localized and characterized by hypersensitivity to a stimulus such as organ distension. In concert with chronic visceral pain, there is a high comorbidity with stress-related psychiatric disorders including anxiety and depression. The mechanisms linking visceral pain with these overlapping comorbidities remain to be elucidated. Evidence suggests that long term stress facilitates pain perception and sensitizes pain pathways, leading to a feed-forward cycle promoting chronic visceral pain disorders such as irritable bowel syndrome (IBS. Early life stress (ELS is a risk-factor for the development of IBS, however the mechanisms responsible for the persistent effects of ELS on visceral perception in adulthood remain incompletely understood. In rodent models, stress in adult animals induced by restraint and water avoidance has been employed to investigate the mechanisms of stress-induce pain. ELS models such as maternal separation, limited nesting, or odor-shock conditioning, which attempt to model early childhood experiences such as neglect, poverty, or an abusive caregiver, can produce chronic, sexually dimorphic increases in visceral sensitivity in adulthood. Chronic visceral pain is a classic example of gene × environment interaction which results from maladaptive changes in neuronal circuitry leading to neuroplasticity and aberrant neuronal activity-induced signaling. One potential mechanism underlying the persistent effects of stress on visceral sensitivity could be epigenetic modulation of gene expression. While there are relatively few studies examining epigenetically mediated mechanisms involved in visceral nociception, stress-induced visceral pain has been linked to alterations in DNA methylation and histone acetylation patterns within the brain, leading to increased expression of pro-nociceptive neurotransmitters. This review will discuss the potential neuronal pathways and mechanisms responsible for

  19. Stress-Induced Chronic Visceral Pain of Gastrointestinal Origin

    Science.gov (United States)

    Greenwood-Van Meerveld, Beverley; Johnson, Anthony C.

    2017-01-01

    Visceral pain is generally poorly localized and characterized by hypersensitivity to a stimulus such as organ distension. In concert with chronic visceral pain, there is a high comorbidity with stress-related psychiatric disorders including anxiety and depression. The mechanisms linking visceral pain with these overlapping comorbidities remain to be elucidated. Evidence suggests that long term stress facilitates pain perception and sensitizes pain pathways, leading to a feed-forward cycle promoting chronic visceral pain disorders such as irritable bowel syndrome (IBS). Early life stress (ELS) is a risk-factor for the development of IBS, however the mechanisms responsible for the persistent effects of ELS on visceral perception in adulthood remain incompletely understood. In rodent models, stress in adult animals induced by restraint and water avoidance has been employed to investigate the mechanisms of stress-induce pain. ELS models such as maternal separation, limited nesting, or odor-shock conditioning, which attempt to model early childhood experiences such as neglect, poverty, or an abusive caregiver, can produce chronic, sexually dimorphic increases in visceral sensitivity in adulthood. Chronic visceral pain is a classic example of gene × environment interaction which results from maladaptive changes in neuronal circuitry leading to neuroplasticity and aberrant neuronal activity-induced signaling. One potential mechanism underlying the persistent effects of stress on visceral sensitivity could be epigenetic modulation of gene expression. While there are relatively few studies examining epigenetically mediated mechanisms involved in visceral nociception, stress-induced visceral pain has been linked to alterations in DNA methylation and histone acetylation patterns within the brain, leading to increased expression of pro-nociceptive neurotransmitters. This review will discuss the potential neuronal pathways and mechanisms responsible for stress

  20. Designing Visceral, Behavioural and Reflective Products

    Science.gov (United States)

    Aftab, Mersha; Rusli, Helen Agustin

    2017-09-01

    Designers and manufacturers often see consumption as the primary objective of a product - with implications such as discarded products, obsolete wastes, and ecological degradation. The paper aims to find the answer to the question, how emotional design can adapt the discarded and undesirable products into something valuable in a long term? This paper presents a framework combining Chapman's theory and Norman's theory on three levels of emotional design to highlight what long lasting connection with products entails. A design approach is presented combing the Wabi Sabi philosophy that promotes the celebration of decay and damage. This is used as one of the design principles for the experiments conducted on discarded products. Through constant user interaction before, during and after the experiments the evaluation of design as an agent of transformation is done. The user conducted the evaluation based on the Kansei elements of looks, sound, smell, and feel of the product. The experiments confirmed that a long-term value is only achieved through redesigning and reconstructing the perception of people towards products on a reflective level, rather than the visceral and behavioural elements of the product. The research found attachment to the visceral and behavioural elements of a product instead of an emotional one was causing users to discard products faster than required. The research indicated that many people, including designers and manufacturers, are unconsciously focusing on usability (behavioural level) and physical look (visceral level) of a product that are easily replaced, than on a meaningful way (reflective level) to create and maintain long-lasting emotions. The research concluded with a proposition towards digitization of products which could perhaps be an all round solution to make products more appropriate to human emotions. Digitization could give products the ability to capture, store and then communicate the stories, journey and memories back, in

  1. Flow measurements in the major visceral arteries

    Energy Technology Data Exchange (ETDEWEB)

    Schroeder, J.; Gerhardt, P.; Terwey, B.; Krastel, A.

    1982-02-01

    Arterial flow was measured by the spill-over technique in 202 coeliac arteries and 158 superior mesenteric arteries during the course of visceral angiography in 250 patients. In the coeliac artery a mean value of 15.8 +- 4.3 ml/sec was obtained, flow in the super mesenteric artery was significantly less at 12.1 +- 3.4 ml/sec. The wide variation from 4 to 26 ml/sec in the coeliac artery was remarkable, as well as flows from six to 20 ml/sec in the superior mesenteric artery.

  2. Immune Regulation during Chronic Visceral Leishmaniasis

    Science.gov (United States)

    Faleiro, Rebecca J.; Kumar, Rajiv; Hafner, Louise M.; Engwerda, Christian R.

    2014-01-01

    Visceral leishmaniasis is a chronic parasitic disease associated with severe immune dysfunction. Treatment options are limited to relatively toxic drugs, and there is no vaccine for humans available. Hence, there is an urgent need to better understand immune responses following infection with Leishmania species by studying animal models of disease and clinical samples from patients. Here, we review recent discoveries in these areas and highlight shortcomings in our knowledge that need to be addressed if better treatment options are to be developed and effective vaccines designed. PMID:25010815

  3. A etnografia das ruas: notas sobre estudar e ser estudado

    Directory of Open Access Journals (Sweden)

    Hecht, Tobias

    2002-01-01

    Full Text Available Passeando entre as notas e as lembranças de dois períodos distintos de trabalho de campo, este ensaio "impressionista" trata da dinâmica em mudança de uma relação de pesquisa com crianças de rua. Em 1992-1993, conduzi trabalho de campo para uma dissertação sobre crianças de rua no nordeste brasileiro. Retornando à região seis anos mais tarde, descobri que a maioria das crianças estudadas apenas uma fração de geração antes estavam então mortas. Estas anotações enfocam minhas conversas com um dos sobreviventes e com quem (e sobre quem estou agora escrevendo uma biografia. Esse esforço colaborativo, voltado de certo modo a reconsiderar as implicações éticas da pesquisa com crianças que levam vidas violentas e vulneráveis, levanta uma gama de novos desafios éticos, alguns dos quais inteiramente imprevistos

  4. Acuidade visual: Medidas e notações

    Directory of Open Access Journals (Sweden)

    Bicas Harley E. A.

    2002-01-01

    Full Text Available Avaliações da função visual são muito complexas por dependerem de mecanismos aferentes, eferentes e cognitivos, além de fatores externos à pessoa examinada, como o tipo de estímulo e o de sua apresentação. O exame da acuidade visual é discutido em seus aspectos formais de definições, quantificações (critérios de medição de um ângulo e tamanho dos optotipos, notações (decimal ou fracionárias, escalas (representando relações angulares, lineares, logarítmicas e unidades em que os valores são expressos (recíproca do minuto de arco, número puro, freqüência espacial, decibéis e oitavas. Como conseqüência, referências numéricas sobre a acuidade visual e operações que as envolvem (p.ex., cálculo de valores médios, determinação de variações, relações entre elas podem levar a interpretações muito diferentes e até opostas num mesmo estudo, dependendo dos critérios nele empregados.

  5. diversos factores de riesgo: nota técnica

    Directory of Open Access Journals (Sweden)

    Francisco López Herrera

    2001-01-01

    Full Text Available Dada la aparente ausencia de una solución explícita para la selección de portafolios óptimos con activos riesgosos cuando el proceso generador de datos (PGD de los rendimientos obedece a una relación lineal con múltiples factores, en esta nota se presenta una propuesta de solución. Se muestra que bajo los supuestos de un modelo multifactorial es posible recurrir a la teoría clásica de optimización para determinar la combinación óptima de activos para reducir el riesgo de un portafolio y administrar su exposición a la influencia de los factores causales de riesgo sistemático. El método de optimización se basa en la derivación de la frontera eficiente que se presenta en Merton (1972 y es consistente con los modelos multifactoriales teóricos, como el APT de Ross (1976 u otros empíricos, siempre y cuando supongan que el PGD obedece a una estructura lineal multivariada. Se presenta también un ejemplo pequeño de la aplicación de esta propuesta a la formación de un portafolios que minimiza el riesgo bajo metas del nivel de su exposición a los factores de riesgo

  6. Inspannen tegen XTC: Eerste meting: stand van zaken 2002 nota 'Samenspannen tegen XTC'

    OpenAIRE

    Snippe, J.; Kruize, A; Bieleman, B.

    2003-01-01

    Doel van dit onderzoek is na te gaan hoe het staat met de inspanningen en de resultaten van de aanpak van synthetische drugs/XTC in 2002, het beginjaar van de intensivering van de aanpak volgens de nota “Samenspannen tegen XTC”? Het betreft de baselinemeting van het evaluatieonderzoek. De nota bevat een breed scala aan beleidsvoornemens om te komen tot een aanzienlijke reductie van de productie van en handel in XTC. De volgende vragen komen in dit rapport aan de orde:Welke inspanningen (input...

  7. Intrathecal tramadol versus intrathecal fentanyl for visceral pain ...

    African Journals Online (AJOL)

    2013-10-29

    Oct 29, 2013 ... local anesthetic agents during management of visceral pain of appendicectomy in Africa in general and in Nigeria in particular. Intrathecal tramadol versus intrathecal fentanyl for visceral pain control during bupivacaine subarachnoid block for open appendicectomy. JM Afolayan, TO Olajumoke1, ...

  8. Autochthonous Outbreak and Expansion of Canine Visceral Leishmaniasis, Uruguay

    Science.gov (United States)

    Satragno, Dinora; Faral-Tello, Paula; Canneva, Bruno; Verger, Lorenzo; Lozano, Alejandra; Vitale, Edgardo; Greif, Gonzalo; Soto, Carlos

    2017-01-01

    We report an outbreak of canine visceral leishmaniasis in Uruguay. Blood specimens from 11/45 dogs tested positive for Leishmania spp. Specimens of Lutzomyia longipalpis sand flies were captured; typing revealed Leishmania infantum. Our findings document an expansion of visceral leishmaniasis to southern South America and risk for vectorborne transmission to humans. PMID:28221113

  9. Genetically modified organisms and visceral leishmaniasis.

    Science.gov (United States)

    Chhajer, Rudra; Ali, Nahid

    2014-01-01

    Vaccination is the most effective method of preventing infectious diseases. Since the eradication of small pox in 1976, many other potentially life compromising if not threatening diseases have been dealt with subsequently. This event was a major leap not only in the scientific world already burdened with many diseases but also in the mindset of the common man who became more receptive to novel treatment options. Among the many protozoan diseases, the leishmaniases have emerged as one of the largest parasite killers of the world, second only to malaria. There are three types of leishmaniasis namely cutaneous (CL), mucocutaneous (ML), and visceral (VL), caused by a group of more than 20 species of Leishmania parasites. Visceral leishmaniasis, also known as kala-azar is the most severe form and almost fatal if untreated. Since the first attempts at leishmanization, we have killed parasite vaccines, subunit protein, or DNA vaccines, and now we have live recombinant carrier vaccines and live attenuated parasite vaccines under various stages of development. Although some research has shown promising results, many more potential genes need to be evaluated as live attenuated vaccine candidates. This mini-review attempts to summarize the success and failures of genetically modified organisms used in vaccination against some of major parasitic diseases for their application in leishmaniasis.

  10. Genetically Modified Organisms and Visceral Leishmaniasis

    Directory of Open Access Journals (Sweden)

    NAHID eALI

    2014-05-01

    Full Text Available Vaccination is the most effective method of preventing infectious diseases. Since the eradication of small pox in 1976, many other potentially life compromising if not threatening diseases have been dealt with subsequently. This event was a major leap not only in the scientific world already burdened with many diseases but also in the mindset of the common man who became more receptive to novel treatment options. Among the many protozoan diseases, the leishmaniases have emerged as one of the largest parasite killers of the world, second only to malaria. There are three types of leishmaniases namely cutaneous (CL, mucocutaneous (ML and visceral (VL, caused by a group of more than 20 species of Leishmania parasites. Visceral leishmaniasis, also known as kala-azar is the most severe form and almost fatal if untreated. Since the first attempts at leishmanization, we have killed parasite vaccines, subunit protein or DNA vaccines, and now we have live recombinant carrier vaccines and live attenuated parasite vaccines under various stages of development. Although some research has shown promising results, many more potential genes need to be evaluated as live attenuated vaccine candidates. This mini-review attempts to summarize the success and failures of genetically modified organisms used in vaccination against some of major parasitic diseases for their application in leishmaniasis.

  11. American visceral Leishmaniasis: a case report

    Directory of Open Access Journals (Sweden)

    H. Langoni

    2005-09-01

    Full Text Available Visceral leishmaniasis is a zoonotic disease caused by parasites of the Leishmania genus. Dog is the major source of infection to man, especially in urban areas. The authors report a case of visceral leishmaniasis in a pit bull female dog from Bocaina, São Paulo, Brazil. The animal presented clinical signs compatible with leishmaniasis, including skin lesions in the body and partial damage of the external ears. The indirect fluorescent antibody test (IFAT demonstrated a titer of 1280, and promastigote forms of Leishmania sp were isolated by the culture of bone marrow puncture. Cytological analysis of the lymph node and smear of the bone marrow puncture revealed macrophages containing amastigote forms of Leishmania sp in their inner region. The test of Polymerase Chain Reaction (PCR utilized the primers LINR4 and LIN19, which amplify 720 base pairs, specific for Leishmania sp. The authors discuss the importance of techniques for a quick and precise diagnosis to this serious zoonosis with great impact in animal and public health.

  12. Correlación de factores pronóstico clínicos, histopatológicos e inmunohistoquímicos en lesiones neoplásicas mamarias malignas en la especie canina

    OpenAIRE

    Pastor Sirvent, Nieves

    2016-01-01

    El objetivo final de este estudio ha sido analizar si la interacción de ciertos factores clínicos, morfológicos e inmunohistoquímicos tienen influencia sobre la evolución del carcinoma mamario en la especie canina. En este trabajo se ha realizado un examen retrospectivo previo sobre 858 pacientes para determinar la incidencia e importancia de la patología oncológica mamaria en las hembras de la especie canina, mediante el análisis de variables epidemiológicas (raza, mestizaje, edad y ta...

  13. Investigation of The Physiological Changes In (Rosa Canina L. Plant Members Comprising and Not Creating The Gall

    Directory of Open Access Journals (Sweden)

    Hülya Özpınar

    2013-04-01

    Full Text Available Chlorophyl a, Chlorophyl b, Carotenoid, total chloropyhl concentrations, Kl-a/Kl-b ratio, Total Chlorophyl/Carotenoid ratio, sugar and starch and total carbohydrate amounts, dry biomass changes and proline and sugar amounts interaction at fruit galls of Diplolepis mayri Schld. On Rosa canina L. were studied. We found that Kl-a, Kl-b, carotenoid concentrations increased but Kla/Kl-b ratio decreased and total chlorophyl/carotenoid ratio did not change with gall occurunce in leaves of galled plants. Sugar content at leaves of galled plants were found to be higher than sugar content of control group with galls. In contrast, starch content decreased. As total carbohydrate amount of galled plants declined with gall occurunce but increased with gall maturation. Generally, we found that dry biomass change and sugar content changes of leaves were similar. Monthly changes of sugar levels in fruits decreased depending on the nutrition of larva in galled fruits. It was also found that there is a similarity between the changes of proline and sugar levels as galls development. Proline levels were found to be very high in galled fruits of plants studied.

  14. NOTAS DE AULA SOBRE PENSAMENTO SOCIAL NO BRASIL: UMA HOMENAGEM A GIRALDA SEYFERTH

    Directory of Open Access Journals (Sweden)

    Ellen Fensterseifer Woortmann

    2015-06-01

    Full Text Available Resenha do livro:  BAHIA, Joana; MENASCHE, Renata; ZANINI, Maria Catarina Chitolina (Org.. Pensamento Social no Brasil, por Giralda Seyferth: notas de aula. Porto Alegre:  Letra&Vida, 2015.   Clique em PDF para abrir o arquivo.

  15. Stress and visceral pain: from animal models to clinical therapies

    Science.gov (United States)

    Larauche, Muriel; Mulak, Agata; Taché, Yvette

    2011-01-01

    Epidemiological studies have implicated stress (psychosocial and physical) as a trigger of first onset or exacerbation of irritable bowel syndrome (IBS) symptoms of which visceral pain is an integrant landmark. A number of experimental acute or chronic exteroceptive or interoceptive stressors induce visceral hyperalgesia in rodents although recent evidence also points to stress-related visceral analgesia as established in the somatic pain field. Underlying mechanisms of stress-related visceral hypersensitivity may involve a combination of sensitization of primary afferents, central sensitization in response to input from the viscera and dysregulation of descending pathways that modulate spinal nociceptive transmission or analgesic response. Biochemical coding of stress involves the recruitment of corticotropin releasing factor (CRF) signaling pathways. Experimental studies established that activation of brain and peripheral CRF receptor subtype 1 plays a primary role in the development of stress-related delayed visceral hyperalgesia while subtype 2 activation induces analgesic response. In line with stress pathways playing a role in IBS, non-pharmacologic and pharmacologic treatment modalities aimed at reducing stress perception using a broad range of evidence-based mind-body interventions and centrally-targeted medications to reduce anxiety impact on brain patterns activated by visceral stimuli and dampen visceral pain. PMID:21575632

  16. Visceral leishmaniasis in zoo and wildlife.

    Science.gov (United States)

    Souza, Tayse Domingues; Turchetti, Andréia Pereira; Fujiwara, Ricardo Toshio; Paixão, Tatiane Alves; Santos, Renato Lima

    2014-03-01

    Visceral leishmaniasis (VL) is an emerging zoonosis caused by Leishmania (Leishmania) infantum. Although the domestic dog is the main vertebrate host, many zoo and wild mammal species have been diagnosed with L. infantum infection, especially in endemic areas. There are many available diagnostic approaches, including serological, parasitological and molecular tests. Among wild animals, carnivores and primates are more often clinically affected, with some species, such as the bush dog (Speothos venaticus) being especially susceptible to development of clinical signs. There are also reports and research articles of VL in felids, rodents, and marsupials. This work aims to review the occurrence of VL in zoo and wildlife and raise awareness of its importance in the field of conservational veterinary medicine. Copyright © 2014 Elsevier B.V. All rights reserved.

  17. Disseminated visceral coccidiosis in sandhill cranes

    Science.gov (United States)

    Carpenter, J.W.; Novilla, M.N.; Fayer, R.; Iverson, G.C.

    1984-01-01

    Disseminated visceral coccidiosis (DVC) caused by Eimeria spp was first recognized as a disease entity in captive sandhill cranes (Grus canadensis) and whooping cranes (G americana) at the Patuxent Wildlife Research Center. Because cranes produced at the Center are reintroduced to the wild to augment wild populations, studies involving both experimentally induced and natural infections were initiated to determine the potential or actual occurrence of DVC in wild Gruidae. Nine sandhill cranes dosed orally with eimerian oocysts of wild origin developed lesions characteristic of DVC. Extraintestinal granulomas associated with developing schizonts were found in 6 birds. Similar lesions were observed in wild sandhill cranes throughout parts of midwestern United States, Alaska, and Saskatchewan. These studies revealed the wide geographic distribution and the high frequency of occurrence of DVC in wild cranes.

  18. [Cutaneous and visceral loxoscelism: a systematic review].

    Science.gov (United States)

    Manríquez, Juan J; Silva, Sergio

    2009-10-01

    Loxoscelism represents a major public health problem for which there are no standard therapeutic interventions. To review available scientific evidence on management of Loxoscelism Systematic review of clinical studies. The search included multiple databases (Medline, Lilacs, Embase, Web of Sciences, Cinahl, Pre-Cinahl, Paperfirst, Proceedingsfirst, Dissertations and Theses, Toxline, Cochrane Library), handsearch of references, and contact with experts. Three clinical trials of poor methodological quality were identified from 5,207 references found. One trial (n = 31), concluded that the use of dapsone was associated with fewer local complications than surgical treatment. A second study (n = 46), concluded that the use of dapsone was superior to clorfenamine for skin lesions. A third study (n = 95) concluded that there was no differences between the use of oral dapsone, antivenom against anti-Loxosceles reclusa or a combination of both. There is insufficient evidence based on good quality studies to recommend treatment guidelines for individuals with skin or visceral loxoscelism.

  19. [Dislipidemia and steatohepatitis with visceral fat].

    Science.gov (United States)

    Yasuda, Daijiro; Maeda, Tomomi; Teramoto, Tamio

    2009-02-01

    The prevalence of metabolic syndrome has been increased recently because of westernized dietary habits and low physical activity in Japan. Metabolic syndrome is diagnosed by the accumulation of dislipidemia, glucose intolerance and/or high blood pressure caused by visceral obesity. The dislipidemia in metabolic syndrome is characterized by the presence of high plasma triglyceride and low HDL-cholesterol, which are associated with increase in small dense LDL and remnant lipoproteins, highly atherogenic lipoproteins. Metabolic syndrome is also often associated with fatty liver, which may be led to non-alcoholic steatohepatitis (NASH). Reduction of body weight and increase in physical activities are highly recommended in overweight patients to inhibit the development of metabolic syndrome, the dislipidemia and NASH.

  20. Stability analysis of glutamic acid linked peptides coupled to NOTA through different chemical linkages.

    Science.gov (United States)

    Lang, Lixin; Ma, Ying; Kiesewetter, Dale O; Chen, Xiaoyuan

    2014-11-03

    Glutamic acid is a commonly used linker to form dimeric peptides with enhanced binding affinity than their corresponding monomeric counterparts. We have previously labeled NOTA-Bn-NCS-PEG3-E[c(RGDyK)]2 (NOTA-PRGD2) [1] with [(18)F]AlF and (68)Ga for imaging tumor angiogenesis. The p-SCN-Bn-NOTA was attached to E[c(RGDyK)]2 [2] through a mini-PEG with a thiourea linkage, and the product [1] was stable at radiolabeling condition of 100 °C and pH 4.0 acetate buffer. However, when the same p-SCN-Bn-NOTA was directly attached to the α-amine of E[c(RGDfK)]2 [3], the product NOTA-Bn-NCS-E[c(RGDfK)]2 [4] became unstable under similar conditions and the release of monomeric c(RGDfK) [5] was observed. The purpose of this work was to use HPLC and LC-MS to monitor the decomposition of glutamic acid linked dimeric peptides and their NOTA derivatives. A c(RGDyK) [6] and bombesin (BBN) [7] heterodimer c(RGDyK)-E-BBN [8], and a dimeric bombesin E(BBN)2 [9], both with a glutamic acid as the linker, along with a model compound PhSCN-E[c(RGDfK)] [10] were also studied. All the compounds were dissolved in 0.5 M pH 4.0 acetate buffer at the concentration of 1 mg/mL, and 0.1 mL of each sample was heated at 100 °C for 10 min and the more stable compounds were heated for another 30 min. The samples at both time points were analyzed with analytical HPLC to monitor the decomposition of the heated samples. The samples with decomposition were further analyzed by LC-MS to determine the mass of products from the decomposition for possible structure elucidation. After 10 min heating, the obvious release of c(RGDfK) [5] was observed for NOTA-Bn-NCS-E[c(RGDfK)]2 [4] and Ph-SCN-E[c(RGDfK)] [10]. Little or no release of monomers was observed for the remaining samples at this time point. After further heating, the release of monomers was clearly observed for E[c(RGDyK)]2 [2], E[c(RGDfK)]2 [3], c(RGDyK)-E-BBN [8], and E(BBN)2 [9]. No decomposition or little decomposition was observed for NOTA

  1. Spironolactone increases permeability of visceral sheep peritoneum.

    Science.gov (United States)

    Karioti, Aggeliki; Hatzoglou, Chrissi; Zarogiannis, Sotirios; Deligiorgi, Triantafyllia; Kourti, Panagiota; Giannopoulou, Myrto; Arampatzis, Spyros; Liakopoulos, Vassilios; Gourgoulianis, Konstantinos; Molyvdas, Paschalis-Adam; Stefanidis, Ioannis

    2009-01-01

    Aldosterone is a key component of the renin-angiotensin-aldosterone system, and spironolactone, an aldosterone receptor blocker, shows beneficial effects in patients with end-stage renal disease and heart failure. The aim of the present study was to investigate by means of Ussing chamber technique the effect of spironolactone on the transmesothelial permeability of visceral sheep peritoneum in vitro. Peritoneal samples from the omentum of adult sheep were collected immediately after slaughter in a cooled and oxygenated Krebs-Ringer bicarbonate (KRB) solution. Isolated intact sheets of peritoneum were mounted in an Ussing-type chamber. Spironolactone (10(-5) mol/L) was added apically and basolaterally to the KRB solution. The transmesothelial resistance (R) was measured before and serially for 30 minutes after the addition of the substances. Data present the mean +/- standard error of 6 experiments in each case. The control R was 19.8 +/- 0.36 omega x cm2. The addition of spironolactone resulted in a reduction in the R, which became significant on both sides of the membrane within 10 minutes and remained significantly different thereafter. The maximum reduction of R (deltaR%) reached 24.8% +/- 2.3% (p < 0.01) apically and 26.3% +/- 3.2% (p < 0.01) basolaterally. Our data clearly show that spironolactone increases the permeability of visceral sheep peritoneum in a lasting manner. Increased peritoneal permeability could result in increased sodium removal, which has acknowledged beneficial effects both in patients undergoing peritoneal dialysis and in patients with heart failure. Further clinical studies investigating the effect of spironolactone on sodium removal in peritoneal dialysis are justified.

  2. Ancient origin of somatic and visceral neurons

    Science.gov (United States)

    2013-01-01

    Background A key to understanding the evolution of the nervous system on a large phylogenetic scale is the identification of homologous neuronal types. Here, we focus this search on the sensory and motor neurons of bilaterians, exploiting their well-defined molecular signatures in vertebrates. Sensorimotor circuits in vertebrates are of two types: somatic (that sense the environment and respond by shaping bodily motions) and visceral (that sense the interior milieu and respond by regulating vital functions). These circuits differ by a small set of largely dedicated transcriptional determinants: Brn3 is expressed in many somatic sensory neurons, first and second order (among which mechanoreceptors are uniquely marked by the Brn3+/Islet1+/Drgx+ signature), somatic motoneurons uniquely co-express Lhx3/4 and Mnx1, while the vast majority of neurons, sensory and motor, involved in respiration, blood circulation or digestion are molecularly defined by their expression and dependence on the pan-visceral determinant Phox2b. Results We explore the status of the sensorimotor transcriptional code of vertebrates in mollusks, a lophotrochozoa clade that provides a rich repertoire of physiologically identified neurons. In the gastropods Lymnaea stagnalis and Aplysia californica, we show that homologues of Brn3, Drgx, Islet1, Mnx1, Lhx3/4 and Phox2b differentially mark neurons with mechanoreceptive, locomotory and cardiorespiratory functions. Moreover, in the cephalopod Sepia officinalis, we show that Phox2 marks the stellate ganglion (in line with the respiratory — that is, visceral— ancestral role of the mantle, its target organ), while the anterior pedal ganglion, which controls the prehensile and locomotory arms, expresses Mnx. Conclusions Despite considerable divergence in overall neural architecture, a molecular underpinning for the functional allocation of neurons to interactions with the environment or to homeostasis was inherited from the urbilaterian ancestor by

  3. Eugenol derived immunomodulatory molecules against visceral leishmaniasis.

    Science.gov (United States)

    Charan Raja, Mamilla R; Velappan, Anand Babu; Chellappan, Davidraj; Debnath, Joy; Kar Mahapatra, Santanu

    2017-10-20

    Visceral leishmaniasis (VL) is a life threatening infectious disease caused by Leishmania donovani. It leads to the severe immune suppression in the host defense system. Higher cytotoxicity, rigorous side effects and lower therapeutic indexes (TI) of current antileishmanial drugs have created a necessity to develop new molecules with better antileishmanial activity and high TI value. In this study, we have synthesized 36 derivatives of eugenol and screened them for their activity against promastigote and amastigote forms of L. donovani. Among the synthesized derivatives, comp.35 showed better antileishmanial activity against extra cellular promastigotes (IC50- 20.13 ± 0.91 μM) and intracellular amastigotes (EC50-4.25 ± 0.26 μM). The TI value (82.24 ± 3.77) was found to improve by 10-13 fold compared to Amphotericin B and Miltefosine respectively. Treatment with comp.35 (5 μg/ml) enhanced the nitric oxide (NO) generation, iNOS2 mRNA expression (∼8 folds increase) and decreased the arginase-1 activity (∼4 folds) in L. donovani infected peritoneal macrophages. Comp.35 had also increased the IL-12 (∼6 folds) and decreased the IL-10 (∼3 folds) mRNA expression and release in vitro. Results of in vivo studies revealed that comp.35 treatment at 25 mg/kg body weight efficiently cleared the hepatic and splenic parasite burden with enhanced Th1 response in L. donovani infected BALB/c mice. Hence, this study clearly represents comp.35, as an immunomodulatory molecule, can induce host protective immune response against visceral leishmaniasis through enhanced NO generation and Th1 response, which are essentials against this deadly disease. Copyright © 2017 Elsevier Masson SAS. All rights reserved.

  4. Causes of pediatric visceral leishmaniasis in southeastern iran.

    Directory of Open Access Journals (Sweden)

    Ali Hosseininasab

    2014-12-01

    Full Text Available Leishmania infantum is the most frequent cause of visceral leishmaniasis and L. tropica has been rarely linked to the disease in Iran. In this study, bone marrow aspirates were collected from 10 child patients, suspected with visceral leishmaniasis referred to the Pediatric Wards of Kerman Medical Hospitals, Kerman, Iran during 2002-2011. Leishmania species were identified by using nested PCR in all slides. The PCR samples from nine patients indicated L. infantum as principal causative agent of visceral leishmaniasis and one L.tropica as a minor species.

  5. RcRR1, a Rosa canina type-A response regulator gene, is involved in cytokinin-modulated rhizoid organogenesis.

    Directory of Open Access Journals (Sweden)

    Bin Gao

    Full Text Available In vitro, a new protocol of plant regeneration in rose was achieved via protocorm-like bodies (PLBs induced from the root-like organs named rhizoids that developed from leaf explants. The development of rhizoids is a critical stage for efficient regeneration, which is triggered by exogenous auxin. However, the role of cytokinin in the control of organogenesis in rose is as yet uncharacterized. The aim of this study was to elucidate the molecular mechanism of cytokinin-modulated rhizoid formation in Rosa canina. Here, we found that cytokinin is a key regulator in the formation of rhizoids. Treatment with cytokinin reduced callus activity and significantly inhibited rhizoid formation in Rosa canina. We further isolated the full-length cDNA of a type-A response regulator gene of cytokinin signaling, RcRR1, from which the deduced amino acid sequence contained the conserved DDK motif. Gene expression analysis revealed that RcRR1 was differentially expressed during rhizoid formation and its expression level was rapidly up-regulated by cytokinin. In addition, the functionality of RcRR1 was tested in Arabidopsis. RcRR1 was found to be localized to the nucleus in GFP-RcRR1 transgenic plants and overexpression of RcRR1 resulted in increased primary root length and lateral root density. More importantly, RcRR1 overexpression transgenic plants also showed reduced sensitivity to cytokinin during root growth; auxin distribution and the expression of auxin efflux carriers PIN genes were altered in RcRR1 overexpression plants. Taken together, these results demonstrate that RcRR1 is a functional type-A response regulator which is involved in cytokinin-regulated rhizoid formation in Rosa canina.

  6. Seguridad ciudadana al nivel local: notas de trabajo de campo sobre la ejecucion y percepcion del 'Plan Cuadrante' en Cali

    National Research Council Canada - National Science Library

    Roman Munoz, Myriam Janneth

    2013-01-01

    Este articulo recoge las notas de trabajo de campo sobre las acciones que la Policia Metropolitana de Cali realiza en el marco del 'Plan Cuadrante' y la percepcion ciudadana ante dicha intervencion...

  7. ATIVIDADE ANTIMICROBIANA DO EXTRATO DE PRÓPOLIS FRENTE A ISOLADOS DE STAPHYLOCOCCUS COAGULASE POSITIVA E MALASSEZIA PACHYDERMATIS DE OTITE CANINA

    OpenAIRE

    Rosemari Laura Cardoso

    2009-01-01

    O objetivo do presente estudo foi avaliar o potencial antimicrobiano do extrato de própolis, substância natural conhecida pelas suas propriedades terapêuticas, frente a 67 isolados microbianos da otite canina. A Concentração Bactericida Mínima (CBM), do extrato de própolis, frente a isolados de Staphylococcus coagulase positiva e a Concentração Fungicida Mínima (CFM) para isolados de Malassezia pachydermatis foram determinadas utilizando-se a técnica de microdiluição em caldo. A CBM foi...

  8. Avances en nuevos marcadores de daño renal en el diagnóstico y tratamiento de la leishmaniosis canina

    OpenAIRE

    Guerrero Cabrera, Samantha

    2017-01-01

    La leishmaniosis canina (CanL, canine leishmaniosis) es una zoonosis endémica transmitida por vectores. La enfermedad renal es una de las principales complicaciones y causas de mortalidad en la CanL. En la leishmaniosis humana se ha observado disfunción renal tubular asociada con defectos en la concentración de orina y con desequilibrios electrolíticos. Las pruebas de laboratorio comúnmente utilizadas para evaluar la función renal carecen de una adecuada sensibilidad y especificidad, ...

  9. AGLUTINACIÓN EN LÁTEX, ELISA Y HEMOAGLUTINACIÓN: ALTERNATIVAS PARA EL DIAGNÓSTICO DE LA PARVOVIROSIS CANINA EN HECES

    OpenAIRE

    S Ariza; D Fuentes; VJ Vera; LC Villamil; GC Ramírez

    2005-01-01

    En vista de su alta morbilidad y letalidad, se han desarrollado varios métodos para el diagnóstico de la parvovirosis canina. Los más utilizados se basan en la detección de partículas virales excretadas en las heces durante la fase aguda de la enfermedad. Algunos requieren equipos especializados, lo que aumenta los costos y el tiempo de diagnóstico. Por esto, la simplificación de los métodos de diagnóstico con alta sensibilidad y especificidad es prioritaria. Este trabajo presenta el desarrol...

  10. Evaluación de Cuatro Protocolos de Anestesia sobre las Variaciones en las Funciones Vitales en la Ovariohisterectomía Canina

    OpenAIRE

    Huayta Huanca, Jhon David; Escuela Profesional de Medicina Veterinaria y Zootecnia, Facultad de Ciencias e Ingenierías Biológicas y Químicas, Universidad Católica de Santa María, Arequipa

    2016-01-01

    El presente estudio tuvo como objetivo la evaluación de los efectos de cuatro protocolos de anestesia sobre las funciones vitales de frecuencia cardiaca, pulso, frecuencia respiratoria, saturación de oxígeno y temperatura corporal en la ovariohisterectomía canina. Se seleccionaron 32 perras adultas (1-8 años), clínicamente sanas, sin distinción de peso ni raza, y clasificados como ASA I (84.4%) o II (15.6%) para ser sometidos a cirugía electiva (no emergencias). Los canes fueron distribuidos ...

  11. Estudio histológico, inmuno-histoquímico y de cultivo celular de mamas humana y canina normal y cancerosa

    Directory of Open Access Journals (Sweden)

    Carine Fernandes B.

    2012-08-01

    Full Text Available Objetivo. Comparar las características morfológicas de células normales y cancerosas derivadas de glándulas mamarias de las especies humana y canina. Materiales y métodos. Fueron colectadas 10 muestras de parénquima mamario normal y 14 de parénquima tumoral de la especie canina, así como seis muestras de parénquima mamario normal y tres de parénquima tumoral de la especie humana. Para el cultivo de células fue utilizada la técnica de cultivo de células de mamíferos y para la histoquímica la técnica de la avidina- biotina- peroxidasa, con utilización del anticuerpo monoclonal 32-2B (anti-desmogléina-1. Resultados. A los estudios histológicos y morfológicos las células de la glándula mamaria normal humana demostraron semejanzas con las células de la glándula mamaria normal canina. Se observó adhesión y proliferación de células normales en ambas especies por aproximadamente tres meses de cultivo. Las células normales de las especies humana y canina mostraron baja actividad de proliferación cuando se compararon con las cancerosas de las especies en estudio. Los grupos de células epiteloides que se adhirieron al sustrato de los dos cultivos tuvieron dependencia de las células estromales, porque en la medida que las células fibroblastoides se retiraron, las células epiteloides detuvieron su crecimiento. Conclusiones. Comparando los resultados obtenidos por medio de análisis inmuno-histoquímico de células normales de mujeres y perras fue posible observar cambios en el patrón de coloración en células cancerosas en ambas especies, demostrando que las estructuras relacionadas con la adhesión celular (desmosomas pueden estar alteradas.

  12. Manejo clínico y diagnóstico terapéutico de un caso presuntivo de anemia hemolítica inmunomediada canina

    OpenAIRE

    Solano-Rodríguez, José Pablo; Suárez-Esquivel, Marcela; Astorga-Miranda, Nancy

    2015-01-01

    Las enfermedades autoinmunes (EAI) continúan representando un gran reto diagnóstico en medicina veterinaria y pueden ser confundidas fácilmente con otras patologías. El objetivo de este trabajo es ilustrar dicha situación con un caso presuntivo de anemia hemolítica autoinmune canina, una de las EAI más frecuente en caninos. Este caso fue atendido en el Hospital de Especies Menores y Silvestres de la Escuela de Medicina Veterinaria de la Universidad Nacional.Se recibió una hembra Pastor Austra...

  13. Visceral pain originating from the upper urinary tract

    DEFF Research Database (Denmark)

    Pedersen, Katja Venborg; Drewes, Asbjørn Mohr; Frimodt-Møller, Poul Christian

    2010-01-01

    -visceral hyperalgesia. However, further studies are needed to better understand these visceral pain mechanisms with regard to optimising pain management. This review gives an introduction to visceral pain in general and upper urinary tract pain in particular, with special reference to pain pathways and pharmacological......Pain originating from the upper urinary tract is a common problem and stone colic is one of the most intense pain conditions that can be experienced in the clinic. The pain is difficult to alleviate and often leads to medical attention. In humans, pain mechanisms of the upper urinary tract pain...... are still poorly understood, which often leads to a trial and error approach in clinical pain management. Pain from the upper urinary tract seems to have all the characteristics of pure visceral pain, including referred pain with or without hyperalgesia/trophic changes in somatic tissues and viscero...

  14. Visceral metabolism and efficiency of energy use by ruminants

    National Research Council Canada - National Science Library

    Gilberto Vilmar Kozloski; João Batista Teixeira da Rocha; Maria de Lourdes Santorio Ciocca

    2001-01-01

    ... absorbed at the intestinal lumen. Probably, the major source of variation in the efficiency of utilization of metabolizable energy among feeds is associated mainly with visceral metabolism and it is unlikely that the ratio ketogenic...

  15. [Visceral leishmaniasis without splenomegaly. A pediatric case report].

    Science.gov (United States)

    Leblanc, C; Nouar, D; Izri, A; Brun, S; Marty, P; Gaudelus, J; De Pontual, L

    2016-04-01

    Pediatric visceral leishmaniasis is caused by Leishmania infantum, a dog parasite transmitted to humans by the bite of the female phlebotomine sand fly. The well-known clinical triad is fever, pallor, and splenomegaly. A secondary macrophage activation syndrome (MAS) can complicate this infection, which is lethal when not treated. When MAS is observed without any explanation, a visceral leishmaniasis is highly recommended. We report a case of visceral leishmaniasis in a 21-month-old child complicated by a macrophage activation syndrome without splenomegaly. No immunodeficiency was diagnosed that could explain this unusual clinical condition. To our knowledge, this is the first case of visceral leishmaniasis without splenomegaly reported to date. Copyright © 2015 Elsevier Masson SAS. All rights reserved.

  16. Congenitally transmitted visceral leishmaniasis: report of two brazilian human cases

    Directory of Open Access Journals (Sweden)

    Myrlena Regina Machado Mescouto-Borges

    2013-04-01

    Full Text Available Visceral leishmaniasis is a relevant public health problem worldwide. Most of the reported cases in Latin America are from Brazil. Herein we report two human cases of congenitally transmitted visceral leishmaniasis in two patients who developed symptoms during pregnancy. The diagnosis was made by visual examination of Leishmania parasites in bone marrow aspirates of the mothers and by detecting parasite kDNA in bone marrow samples of the newborn children using polymerase chain reaction.

  17. Role of Principal Ionotropic and Metabotropic Receptors in Visceral Pain

    OpenAIRE

    Kannampalli, Pradeep; Sengupta, Jyoti N.

    2015-01-01

    Visceral pain is the most common form of pain caused by varied diseases and a major reason for patients to seek medical consultation. It also leads to a significant economic burden due to workdays lost and reduced productivity. Further, long-term use of non-specific medications is also associated with side effects affecting the quality of life. Despite years of extensive research and the availability of several therapeutic options, management of patients with chronic visceral pain is often in...

  18. Emergency endovascular repair of ruptured visceral artery aneurysms

    Directory of Open Access Journals (Sweden)

    Tang Tjun

    2007-07-01

    Full Text Available Abstract Background Visceral artery aneurysms although rare, have very high mortality if they rupture. Case presentation An interesting case of a bleeding inferior pancreaticduodenal artery aneurysm is reported in a young patient who presented with hypovolemic shock while being treated in the hospital after undergoing total knee replacement. Endovascular embolization was successfully employed to treat this patient, with early hospital discharge. Conclusion Prompt diagnosis and endovascular management of ruptured visceral aneuryms can decrease the associated mortality and morbidity.

  19. Effects of orlistat on visceral fat after liposuction.

    Science.gov (United States)

    Montoya, Teresa; Monereo, Susana; Olivar, Juana; Iglesias, Paloma; Díaz, Patricia

    2009-03-01

    Liposuction can aggravate metabolic complications associated with obesity. It has been shown that the recovery of weight lost through these interventions is associated with body fat redistribution toward the visceral cavity, increasing metabolic risk factors for coronary heart disease such as insulin resistance and high triglyceride levels. The aim of this study was to evaluate the consequences of liposuction on body mass redistribution and metabolic parameters 6 months after surgery and to evaluate the use of orlistat treatment (tetrahydrolipstatin) in controlling these parameters. A population of 31 women with a mean body mass index of 26.17+/-3.9 kg/m(2) and undergoing liposuction of more than 1,000 cm(3), was studied. Twelve of them were treated postsurgery with 120 mg of orlistat every 8 hours for the following 6 months. Anthropometric, analytical, and radiological (computed tomography) tests were performed to quantify visceral fat area before surgery and 6 months after surgery. Despite weight loss after liposuction, visceral fat was not modified. Patients treated with orlistat showed a greater reduction in visceral fat, although not statistically significant. Orlistat use induced a reduction in low-density lipoprotein cholesterol values of 20.0+/-22.5 mg/dL, compared with an increase of 8.46+/-20.1 mg/dL in controls (p=.07). Visceral fat does not decrease despite weight loss after liposuction. Orlistat use postliposuction might be a useful tool because it shows a tendency to reduce visceral fat and improve blood lipids profile.

  20. Hyponatremia in visceral leishmaniasis Hiponatremia no calazar

    Directory of Open Access Journals (Sweden)

    Frederico A. Lima Verde

    2010-10-01

    Full Text Available There are few reports linking hyponatremia and visceral leishmaniasis (kala-azar. This is a study of 55 consecutive kala-azar patients and 20 normal individuals as a control group. Hyponatremia and serum hypo-osmolality were detected in 100% of kala-azar patients. High first morning urine osmolality (750.0 ± 52.0 vs. 894.5 ± 30.0mOsm/kg H2O, p Existem poucos relatos relacionando hiponatremia com a leshmaniose visceral (calazar. Este é um estudo de 55 pacientes portadores de calazar e um grupo controle de 20 indivíduos normais. Hiponatremia e hipo-osmolalidade sérica foram detectados em 100% dos pacientes portadores de calazar. A presença de alta osmolalidade da primeira urina da manhã (750,0 ± 52,0 vs. 894,5 ± 30 mOsm/Kg H2O, p < 0,05 e da urina de 24h (426,0 ± 167,0 vs. 514,6 ± 132,0 mOsm/Kg H2O, p < 0,05, demonstraram a presença de persistente secreção de hormônio antidiurético. A concentração de sódio urinário foi elevada (82,3 ± 44,2 vs. 110,3 ± 34,7 mEq/L, p < 0,05. Hipouricemia ocorreu em 61,8% dos pacientes e aumento da fração de excreção urinária de ácido úrico foi detectada em 74,5% dos casos. Aumento da velocidade de filtração glomerular estava presente em 25,4% dos pacientes. Não havia evidência clínica de depleção de volume extracelular. Valores normais de ADH plasmático foram observados nos pacientes com calazar. Não foi detectada disfunção renal ou endócrina. É provável, que a maioria dos pacientes com calazar apresente uma síndrome de secreção inapropriada de hormônio antidiurético.

  1. Significantly increased visceral adiposity index in prehypertension.

    Directory of Open Access Journals (Sweden)

    Yanan Ding

    Full Text Available The prevalence of prehypertension has increased in China, and prehypertension frequently progress to hypertension over a short time period; both have become public health problems. Therefore, this study was conducted to determine the relationship between the Visceral Adiposity Index (VAI and blood pressure (BP in China.A cross-sectional epidemiological survey was conducted in China using a stratified random cluster sampling method. Sex-specific VAI quartile cut-off points were used as follows: 0.88, 1.41, 2.45 in males and 0.85, 1.33, 2.22 in females. Prehypertension and hypertension were each defined according to The Seventh Report of the Joint National Committee on the Prevention, Detection, Evaluation, and Treatment of High Blood Pressure (JNC 7 guidelines. A multivariate logistic analysis was conducted to analyze the relationship among VAI, prehypertension and hypertension.The ORs for prehypertension and hypertension in the upper quartiles of the VAI were 1.514 (1.074-2.133, P=0.018 and 1.660 (1.084-2.542, P=0.020, in males, after adjusting for age, education, smoking habits, alcohol consumption, physical activity, serum creatinine, fasting glucose, and plasma insulin. Following further adjustments for the above confounders, chronic kidney disease, and diabetes, the ORs for prehypertension and hypertension in the upper quartile of the VAI were 1.660 1.533 (1.086-2.165, P=0.015, and 1.743 (1.133-2.680, P=0.011, in males. The ORs for prehypertension and hypertension in the upper quartile of the VAI were 1.691 (1.223-2.338, P=0.001, and 1.682 (1.162-2.435, P=0.006, in females, after adjusting for age, education, smoking habits, alcohol consumption, physical activity, serum creatinine, fasting glucose, and plasma insulin. Following further adjustments for the above confounders, chronic kidney disease, and diabetes, the ORs for prehypertension and hypertension in the upper quartile of the VAI were 1.688 (1.220-2.334, P=0.002, and 1.657 (1

  2. Estudo soroepidemiológico de leishmaniose em equinos na região de Araçatuba-SP, Brasil, área endêmica para leishmaniose visceral

    Directory of Open Access Journals (Sweden)

    Francisco Leydson Formiga Feitosa

    2012-12-01

    Full Text Available O presente estudo teve como objetivo pesquisar a presença de anticorpos anti-Leishmania sp. em equinos provenientes do município de Araçatuba-São Paulo, área endêmica para leishmaniose visceral canina e humana. Dos 466 equinos testados para a presença de IgG anti-Leishmania chagasi pelo método de ELISA, 68 (14,59% apresentaram sororeatividade, com títulos variando entre 0,324 e 0,813. As amostras de soro dos equinos positivos por ELISA foram testadas também por imunocromatografia, e 19/466 (4,08% apresentaram resultado positivo. Os resultados obtidos no presente estudo indicaram que esta espécie animal está em contato e deve atrair flebotomíneos transmissores da doença, e salientam a necessidade de investigação mais acurada sobre o real papel dos equinos residentes em áreas endêmicas, de forma a auxiliar nas medidas de controle da doença.

  3. Recommendations on the Fifth Policy Document on Spatial Planning; Advies Vijfde Nota Ruimtelijke Ordening

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-09-21

    In December 2000 the Dutch Cabinet issued Part 1 of the Fifth National Policy Document on Spatial Planning, 'Making space, sharing space'. This is a strategic document, containing the principal options for the future spatial development of the Netherlands. The government asked the Dutch Social and Economic Council (Sociaal-Economische Raad, SER) to advise on a number of the document's main points. The fundamental purpose of the policy should be to promote the optimal allocation of land. Spatial quality, economic development potential, accessibility, and quality of life should jointly enhance the vitality of both urban and rural areas. To this end, development-oriented planning is required. The SER sees planning policy as having three corner stones: facilitating social and economic developments (users' value), promoting the quality of land use (visual qualities), and monitoring future qualities (including sustainability) by carefully managing irreversibles. [Dutch] De Sociaal-Economische Raad (SER) is gevraagd een reactie te geven op de hoofdlijnen van deel 1 van de Nota Ruimte maken, ruimte delen, de Vijfde Nota over de Ruimtelijke Ordening. Daarbij is gevraagd om een algemene beoordeling van de inhoudelijke kern en de sturingsfilosofie van de Vijfde Nota en om aanbevelingen voor de verdere uitwerking van de uitvoeringsstrategie en voor een goede doorwerking van de Vijfde Nota in het beleid van de ruimtelijk relevante sectoren. Daarnaast wordt in het bijzonder aandacht besteed aan een drietal vraagstukken: de kwaliteit van stad en land en de uitwerking van de contourenbenadering; stedelijke netwerken als organiserend principe, en water als organiserend principe. Hoofdstuk 2 geeft in het kort een paar belangrijke hoofdlijnen van de Vijfde Nota weer. In hoofdstuk 3 ontwikkelt de SER zijn visie op ontwikkelingsplanologie. Hoofdstuk 4 gaat in het kader van ontwikkelingsbeelden en organiserende principes in op het watersysteem, op de netwerken van

  4. GC×GC-TOF/MS Chromatographic Analysis, Antioxidant Capacity and Phenolic Content of Rosa Canina L. at Different Maturities

    Directory of Open Access Journals (Sweden)

    Dilek Ozyurt

    2016-01-01

    Full Text Available The relationship between food and health has become increasingly significant as consumers now demand healthy, tasty and natural foods which perform a useful function in the body. In Turkey, rose hip (Rosa canina L. is in popular use in foodstuffs such as marmalade, pestil (a traditional Turkish sweet and syrup or in traditional medicine, mainly as herbal tea. The composition, total antioxidant capacity (TAC and total phenolic content (TPC of volatiles of rose hip samples were compared in relation to their stage of maturity and collection location. Rose hip fruit and seed were analyzed using a direct thermal desorption technique coupled with comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry (GCxGC-TOF/MS. The major components found in the volatile fraction released on extraction were free fatty acids. The various stages of maturity of rose hips (green, light yellow, yellow, orange and red coloured and the place of collection (Istanbul, Mersin, York resulted in some changes in volatile compound speciation, in TAC and TPC levels. Various extraction methods were compared; methanol extraction, traditional hot water infusion and boiling in water. Boiling in water for ten minutes was the method which demonstrated the highest extraction efficiency. TAC and TPC were determined using different electron transfer−based assays; the Cerium (IV ions reducing antioxidant capacity, Cupric Reducing Antioxidant Capacity and Folin-Ciocalteu methods. The highest TAC and TPC were found in the red coloured (fully ripe rose hip fruit, suggesting that colour is a suitable indicator for optimal harvesting time.

  5. Soroepidemiologia da leptospirose canina na região metropolitana de Natal, estado do Rio Grande do Norte

    Directory of Open Access Journals (Sweden)

    Annielle Regina da Fonseca Fernandes

    2013-06-01

    Full Text Available Objetivou-se com este estudo determinar a frequência de cães soropositivos para anticorpos anti-Leptospira spp. no município de Natal e região metropolitana, estado do Rio Grande do Norte, e identificar fatores de risco associados à infecção. Foram examinadas 365 amostras de soro sanguíneo de cães atendidos na rotina de diversas clínicas veterinárias durante o período de março a novembro de 2011. O diagnóstico sorológico da leptospirose foi realizado pela técnica de soroaglutinação microscópica (SAM, utilizando-se 24 sorovares de Leptospira spp. como antígenos. Dos 365 cães, 25 foram soropositivos para pelo menos um dos sorovares de Leptospira spp., com frequência de 6,8%. Os sorovares com reações sorológicas mais frequentes foram Shermani (40%, Sentot (36% e Copenhageni (20%. O perfil epidemiológico da leptospirose canina na região metropolitana de Natal, estado do Rio Grande do Norte, indica que a infecção ocorre com frequência baixa em comparação com outras regiões, provavelmente devido ao grande percentual de animais vacinados, bem como, sugere-se distribuição homogênea de animais soropositivos na região. Por outro lado, a presença de roedores pode ser um importante fator de risco, uma vez que sorovares mantidos por estes animais foram identificados entre os mais frequentes.

  6. Influenza delle misure preventive sulla prevalenza di leishmaniosi canina in un focolaio identificato in Nord Italia nel 2006

    Directory of Open Access Journals (Sweden)

    Rudi Cassini

    2013-06-01

    Full Text Available La leishmaniosi canina è un'infezione endemica nel bacino del Mediterraneo con una documentata tendenza a diffondersi verso nord. L'uso massiccio di misure di protezione contro i rischi del contatto con i flebotomi (collari e formulazioni spot-on è stato studiato in un focolaio registrato in una zona collinare dei Colli Euganei in Italia nord-orientale nel 2006. Nel 2006 e 2007 sono stati testati i sieri di 449 cani provenienti dalla stessa zona. Trentuno animali (6,9% sono risultati positivi al test di immunofluorescenza indiretta (IFI. L'analisi dei fattori di rischio ha chiaramente dimostrato come il focolaio fosse limitato alla località di Calaone, situata nel comune di Baone (PD. Per verificare l'efficacia delle misure preventive adottate, nel 2010, 63 cani di Calaone sono stati campionati e i loro proprietari intervistati. In base alle informazioni rilasciate dai proprietari, la protezione dei cani nei confronti della leishmaniosi ha avuto inizio nel 2006 (66,7% dei cani protetti crescendo negli anni seguenti (90% dei cani protetti. Il valore di sieroprevalenza (4,2% della classe di cani di età più giovane (<5 anni è risultato significativamente minore di quello delle altre classi, dimostrando che gli animali nati dopo il 2006 hanno avuto meno probabilità di infettarsi. Inoltre, i valori di sieroprevalenza riferiti esclusivamente ai cani di Calaone sono risultati del 32,4% (23/71 nel 2006-2007 e 20,6% (13/63 nel 2010, mostrando una tendenza decrescente. I risultati ottenuti mostrano un alto livello di sensibilizzazione dei proprietari dei cani e sembrano evidenziare come l'uso pervasivo di collari e spot-on agisca positivamente nel ridurre l'intensità di circolazione di Leishmania infantum.

  7. Diagnóstico de la ehrlichiosis monocítica canina: una revisión actualizada

    Directory of Open Access Journals (Sweden)

    Roger Iván Rodríguez-Vivas

    2015-01-01

    Full Text Available La ehrlichiosis monocítica canina (EMC es causada por la infección de una rickettsia denominada Ehrlichia canis; este microorganismo se reproduce solamente en el citoplasma de los monocitos sanguíneos y macrófagos tisulares. La EMC ha sido reportada en todo el mundo, pero las frecuencias más altas se reportan en las regiones tropicales y subtropicales. La EMC no siempre es reconocida clínicamente, pues es difícil de diagnosticar debido a que los signos clínicos son variables, a que se presenta en diferentes fases y a la existencia de coinfecciones con otros patógenos transmitidos por garrapatas y otros agentes infecciosos. Las manifestaciones clínicas y las técnicas de diagnóstico tradicionales, como el diagnóstico hematológico y la demostración típica de mórulas de E. canis en el citoplasma de monocitos en frotis sanguíneos, se han utilizado para el diagnóstico de la EMC; sin embargo, las pruebas de diagnóstico por PCR y la serología mediante el uso de antígenos específicos son las pruebas más sensibles y específicas para el diagnóstico de la enfermedad. Este artículo presenta una revisión actualizada sobre el diagnóstico de la infección por E. canis en perros.

  8. Biofabrication of zinc oxide nanoparticles using fruit extract of Rosa canina and their toxic potential against bacteria: A mechanistic approach.

    Science.gov (United States)

    Jafarirad, Saeed; Mehrabi, Meysam; Divband, Baharak; Kosari-Nasab, Morteza

    2016-02-01

    The use of plant extract in the biosynthesis of nanoparticles (NPs) can be an eco-friendly approach and have been suggested as a possible alternative to classic methods namely physical and chemical procedures. In this study, the biosynthesis of zinc oxide (ZnO) NPs by both "conventional heating" (CH) and "microwave irradiation" (MI) methods has been reported. Stable and spherical ZnONPs were produced using zinc nitrate and flesh extract of Rosa canina fruit (rosehip) which was used as a precursor. The flesh extract acts as a reducing and capping agent for generation of ZnONPs. The structural, morphological and colloidal properties of the as-synthesized NPs have been confirmed by X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM), Energy Dispersive X-ray (EDX), Fourier transform Infrared (FT-IR) and Dynamic Light Scattering (DLS). In comparison with the CH method, the MI method has some advantages such as significantly short reaction time (within 8min) owing to the high heating rate and thus the accelerated reaction rate. Both methods led to the synthesis of nearly identical NPs with respect to shape and size according to the results of DLS, XRD and SEM techniques. The possible mechanism for synthesis pathway has been proposed based on FT IR results, XRD patterns, potentiometric data and antioxidant activity. In addition, the antibacterial activity of as-prepared ZnONPs was investigated against several bacteria such as Listeria monocytogenes, Escherichia coli, Salmonella typhimurium. Moreover, the efficacy of ZnONPs to treat cancer cell lines were measured by means of cell viability test via MTT assay in which concentrations of 0.05 and 0.1mg/mL of ZnONPs induced a very low toxicity. Thus, the present investigation reveals that ZnONPs have the potential for various medical and industrial applications. Copyright © 2015 Elsevier B.V. All rights reserved.

  9. Técnica Pasteur São Paulo para dimensionamento de população canina

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    Marina Ruiz de Matos

    2002-10-01

    Full Text Available Este artigo apresenta técnica para dimensionar e classificar populações caninas, segundo graus de dependência e restrição. Foi desenvolvida pelo Instituto Pasteur de São Paulo, com a seguinte metodologia: (1 realização de um inquérito domiciliar para identificação dos cães com proprietário e do grau de restrição. Aos proprietários de cães restritos, totalmente ou parcialmente, e aos moradores que não se definiram como proprietários únicos foram entregues coleiras de três cores distintas, solicitando-lhes colocação nos cães sob seus cuidados; (2 contagem de cães que circulavam na rua e classificação conforme cor da coleira, cuja ausência identificou os cães sem proprietário. Os pesquisadores foram posicionados em locais pré-determinados, distantes em média cinqüenta metros. O tempo de cada contagem foi de trinta segundos, repetindo-se a cada 15 minutos, por uma hora, com início e término marcados por apito. A técnica, aplicada em dois setores censitários do Município de Serra Azul, Estado de São Paulo, Brasil, foi realizada por adolescentes de 13 a 17 anos, submetidos a um dia de treinamento. O procedimento foi simples e possibilitou acesso à informação de difícil obtenção, como o percentual de cães sem proprietário ou sem proprietário definido, que, no caso estudado, foi de 5%.

  10. Técnica Pasteur São Paulo para dimensionamento de população canina

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    Matos Marina Ruiz de

    2002-01-01

    Full Text Available Este artigo apresenta técnica para dimensionar e classificar populações caninas, segundo graus de dependência e restrição. Foi desenvolvida pelo Instituto Pasteur de São Paulo, com a seguinte metodologia: (1 realização de um inquérito domiciliar para identificação dos cães com proprietário e do grau de restrição. Aos proprietários de cães restritos, totalmente ou parcialmente, e aos moradores que não se definiram como proprietários únicos foram entregues coleiras de três cores distintas, solicitando-lhes colocação nos cães sob seus cuidados; (2 contagem de cães que circulavam na rua e classificação conforme cor da coleira, cuja ausência identificou os cães sem proprietário. Os pesquisadores foram posicionados em locais pré-determinados, distantes em média cinqüenta metros. O tempo de cada contagem foi de trinta segundos, repetindo-se a cada 15 minutos, por uma hora, com início e término marcados por apito. A técnica, aplicada em dois setores censitários do Município de Serra Azul, Estado de São Paulo, Brasil, foi realizada por adolescentes de 13 a 17 anos, submetidos a um dia de treinamento. O procedimento foi simples e possibilitou acesso à informação de difícil obtenção, como o percentual de cães sem proprietário ou sem proprietário definido, que, no caso estudado, foi de 5%.

  11. Obesity Takes Its Toll on Visceral Pain: High-Fat Diet Induces Toll-Like Receptor 4-Dependent Visceral Hypersensitivity.

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    Mónica Tramullas

    Full Text Available Exposure to high-fat diet induces both, peripheral and central alterations in TLR4 expression. Moreover, functional TLR4 is required for the development of high-fat diet-induced obesity. Recently, central alterations in TLR4 expression have been associated with the modulation of visceral pain. However, it remains unknown whether there is a functional interaction between the role of TLR4 in diet-induced obesity and in visceral pain. In the present study we investigated the impact of long-term exposure to high-fat diet on visceral pain perception and on the levels of TLR4 and Cd11b (a microglial cell marker protein expression in the prefrontal cortex (PFC and hippocampus. Peripheral alterations in TLR4 were assessed following the stimulation of spleenocytes with the TLR4-agonist LPS. Finally, we evaluated the effect of blocking TLR4 on visceral nociception, by administering TAK-242, a selective TLR4-antagonist. Our results demonstrated that exposure to high-fat diet induced visceral hypersensitivity. In parallel, enhanced TLR4 expression and microglia activation were found in brain areas related to visceral pain, the PFC and the hippocampus. Likewise, peripheral TLR4 activity was increased following long-term exposure to high-fat diet, resulting in an increased level of pro-inflammatory cytokines. Finally, TLR4 blockage counteracted the hyperalgesic phenotype present in mice fed on high-fat diet. Our data reveal a role for TLR4 in visceral pain modulation in a model of diet-induced obesity, and point to TLR4 as a potential therapeutic target for the development of drugs to treat visceral hypersensitivity present in pathologies associated to fat diet consumption.

  12. Visceral leishmaniasis in adults with nephropathy

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    H Kaaroud El Jeri

    2017-01-01

    Full Text Available The aim of this study is to evaluate the features of visceral leishmaniasis (VL in adults with nephropathy, who were not infected with the human immunodeficiency virus. This is a retrospective study of 14 adults hospitalized between 2000 and 2014, with VL and renal involvement. Clinical, biological, and therapeutic data were collected from the patients′ medical files. Eleven women and three men, most of whom were from the North of the country, with a mean age of 40.5 years were studied. Lupus was present in five cases, the Sicca syndrome in three cases, diabetes in one case, renal failure on dialysis in two cases, and there were three renal transplant recipients. Major clinical symptoms were fever and weakness in all cases. Enlargement of the spleen was present in eight cases and hepatomegaly in six cases. Biologic inflammatory syndrome and anemia were present in all cases, and pancytopenia was present in seven cases. Renal insufficiency was noted in all cases. Diagnosis of VL was confirmed by bone marrow examination or serology. Treatment consisted of antimoniate in 10 cases and amphotericin B in four cases. Seven deaths were recorded. Clinical symptoms of VL are atypical in patients with nephropathy and therefore, the diagnosis should be suspected in such patients because VL is still endemic in our country.

  13. Management of visceral leishmaniasis: Indian perspective.

    Science.gov (United States)

    Agrawal, S; Rai, M; Sundar, S

    2005-01-01

    Diagnosis and treatment of Indian visceral leishmaniasis (VL) is extremely unsatisfactory. For diagnosis, demonstration of parasites in splenic/marrow smears remains the gold standard, though k39 rapid strip test is a useful method in regions where access to parasite demonstration is difficult. pentavalent antimony remains the mainstay for the treatment of all forms of leishmaniasis globally; however, development of large-scale antimony resistance in Bihar has necessitated search for alternative drugs. Amphotericin B is the most effective, though toxic, drug for patients with refractory VL. Lipid formulations of amphotericin B, though safe and effective, are too expensive to be useful for poor patients of this region. These hold advantage as large quantity of the drug can safely be given over a short period of time, thus leading to a decrease in the hospital stay to a few days instead of several weeks. Oral miltefosine, an alkyl phospholipid, has recently been approved and marketed in India for the treatment of VL. Miltefosine cures 94% patients with VL if given in a daily dose of 50-100 mg for 28 days. Most common adverse events are mild vomiting and diarrhea. Paromomycin, an amino glycoside, is undergoing a pivotal phase-III clinical trial, and is likely to be approved and available to patients with VL at an affordable cost. To protect the already scarce inventory of antileishmanial drugs, it is time that combination chemotherapy is introduced for the treatment of VL in India.

  14. Management of visceral leishmaniasis: Indian perspective

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    Agrawal S

    2005-01-01

    Full Text Available Diagnosis and treatment of Indian visceral leishmaniasis (VL is extremely unsatisfactory. For diagnosis, demonstration of parasites in splenic/marrow smears remains the gold standard, though k39 rapid strip test is a useful method in regions where access to parasite demonstration is difficult. pentavalent antimony remains the mainstay for the treatment of all forms of leishmaniasis globally; however, development of large-scale antimony resistance in Bihar has necessitated search for alternative drugs. Amphotericin B is the most effective, though toxic, drug for patients with refractory VL. Lipid formulations of amphotericin B, though safe and effective, are too expensive to be useful for poor patients of this region. These hold advantage as large quantity of the drug can safely be given over a short period of time, thus leading to a decrease in the hospital stay to a few days instead of several weeks. Oral miltefosine, an alkyl phospholipid, has recently been approved and marketed in India for the treatment of VL. Miltefosine cures 94% patients with VL if given in a daily dose of 50-100 mg for 28 days. Most common adverse events are mild vomiting and diarrhea. Paromomycin, an amino glycoside, is undergoing a pivotal phase-III clinical trial, and is likely to be approved and available to patients with VL at an affordable cost. To protect the already scarce inventory of antileishmanial drugs, it is time that combination chemotherapy is introduced for the treatment of VL in India.

  15. Colorectal visceral perception in diverticular disease.

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    Clemens, C H M; Samsom, M; Roelofs, J; van Berge Henegouwen, G P; Smout, A J P M

    2004-05-01

    The pathogenesis of asymptomatic diverticular disease (ADD) and symptomatic uncomplicated diverticular disease (SUDD) has not been elucidated. The aim of our study was to assess whether altered visceral perception or abnormal compliance of the colorectal wall play a role in these clinical entities. Ten ADD patients, 11 SUDD patients, and nine healthy controls were studied. Using a dual barostat device, sensations were scored and compliance curves obtained using stepwise intermittent isobaric distensions of the rectum and sigmoid, before and after a liquid meal. In addition, the colonic response to eating was assessed by monitoring the volumes of both barostat bags at operating pressure before and after the meal. In the rectum, perception was increased in the SUDD group compared with controls (p = 0.010) and the ADD group (p = 0.030). Rectal compliance curves were not different between the groups. In the sigmoid colon, perception in the pre- and postprandial periods was increased in SUDD compared with controls (p = 0.018) but not when compared with ADD. Sigmoid volume-pressure curves had comparable slopes (compliance) in all groups but were shifted downwards in SUDD compared with ADD in the preprandial period (p = 0.026). The colonic response to eating (decrease in intrabag volume) was similar in all three groups, both in the rectum and sigmoid. Symptomatic but not asymptomatic uncomplicated diverticular disease is associated with heightened perception of distension, not only in the diverticula bearing sigmoid, but also in the unaffected rectum. This hyperperception is not due to altered wall compliance.

  16. Nanomedicines for Therapy of Visceral Leishmaniasis.

    Science.gov (United States)

    Want, Muzamil Yaqub; Yadav, Priya; Afrin, Farhat

    2016-03-01

    Visceral leishmaniasis (VL) or kala-azar is a vector borne infectious disease caused by the protozoan parasites of the genus Leishmania. VL is endemic in more than 85 countries with an estimated 0.2-0.5 million people at risk, causing high morbidity and mortality across the globe. In the absence of effective vaccines, treatment solely relies on chemotherapy and can be 100% fatal within two years, if left untreated. However, the present chemotherapeutics is limited by toxicity, non-compliance, location of parasites within the lysosomal vacuoles of macrophages, impairing the accession of many potential antileishmanial drugs, prolonged and cumbersome regimen that is unaffordable by rural population with alarming increase in unresponsiveness, complications of post kala-azar dermal leishmaniasis (PKDL) and HIV co-infections. Nanotechnology offers promising approach in the treatment of VL as it reduces toxicity, improves the therapeutic index of drugs, and can selectively deliver the antileishmanial cargos to the intracellular pathogens. In addition, nanoparticles can interact with the host immune system, modulating the immune response in a way that may favor the elimination of the Leishmania parasites. In this review, we give an overview of the strategies and delivery systems employed for the antileishmanial drugs towards the riddance of deadly VL.

  17. Neuroprotective Effects of Herbal Extract (Rosa canina, Tanacetum vulgare and Urtica dioica) on Rat Model of Sporadic Alzheimer’s Disease

    Science.gov (United States)

    Daneshmand, Parvaneh; Saliminejad, Kioomars; Dehghan Shasaltaneh, Marzieh; Kamali, Koorosh; Riazi, Gholam Hossein; Nazari, Reza; Azimzadeh, Pedram; Khorram Khorshid, Hamid Reza

    2016-01-01

    Background: Sporadic Alzheimer’s Disease (SAD) is caused by genetic risk factors, aging and oxidative stresses. The herbal extract of Rosa canina (R. canina), Tanacetum vulgare (T. vulgare) and Urtica dioica (U. dioica) has a beneficial role in aging, as an anti-inflammatory and anti-oxidative agent. In this study, the neuroprotective effects of this herbal extract in the rat model of SAD was investigated. Methods: The rats were divided into control, sham, model, herbal extract -treated and ethanol-treated groups. Drug interventions were started on the 21st day after modeling and each treatment group was given the drugs by intraperitoneal (I.P.) route for 21 days. The expression levels of the five important genes for pathogenesis of SAD including Syp, Psen1, Mapk3, Map2 and Tnf-α were measured by qPCR between the hippocampi of SAD model which were treated by this herbal extract and control groups. The Morris Water Maze was adapted to test spatial learning and memory ability of the rats. Results: Treatment of the rat model of SAD with herbal extract induced a significant change in expression of Syp (p=0.001) and Psen1 (p=0.029). In Morris Water Maze, significant changes in spatial learning seen in the rat model group were improved in herbal-treated group. Conclusion: This herbal extract could have anti-dementia properties and improve spatial learning and memory in SAD rat model. PMID:27563424

  18. Diagnóstico de criptococose canina pela citologia aspirativa por agulha fina Diagnosis of canine cryptococcosis by fine-needle aspiration cytology

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    Danieli Brolo Martins

    2008-06-01

    Full Text Available Relata-se um caso de criptococose canina de caráter sistêmico e neurológico diagnosticado com o auxílio da citologia aspirativa por agulha fina (CAAF. O paciente, da raça Labrador, 1 ano e 5 meses, macho, apresentava sinais de depressão/estupor, hiporexia, presença de uma massa cervical delimitada entre os dois linfonodos submandibulares e aumento de linfonodos. A punção aspirativa da massa cervical e dos linfonodos poplíteo, pré-escapular e submandibular revelaram presença de fungos compatíveis com Cryptococcus neoformans, o qual foi então confirmado pela cultura fúngica, e classificado como sorotipo D. A CAAF mostrou ser um método rápido, seguro e eficaz em casos de criptococose canina com presença de linfoadenomegalia.This paper aimed to described a 1.5 year-old Labrador male, diagnosed with cryptococcosis using fine-needle aspiration cytology (FNAC. The dog was showing signs of depression and hyporexia. Peripheral lymph nodes and a mass situated between the submandibular lymph nodes were aspirated. The cytology showed yeast-like structures resembling Cryptococcus sp. in all samples examined. These findings were confirmed by culture (C. neoformans, serotype D. The FNAC technique allowed a quick, safe and easy diagnosis in this case.

  19. Estudo de flebotomíneos (Diptera: Psychodidae em foco de leishmaniose visceral no Estado de Mato Grosso do Sul, Brasil Study of phlebotomines (Diptera: Psychodidae in focus of visceral leishmaniasis in Brazil

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    Eunice A. B. Galati

    1997-08-01

    Full Text Available INTRODUÇÃO: Nas Américas, Lutzomyia longipalpis tem sido incriminada como vetora da leishmaniose visceral em, praticamente, todas as áreas de ocorrência dessa parasitose. A notificação de casos humanos a partir de 1980 e a presença de cães com aspecto sugestivo de leishmaniose visceral no Município de Corumbá, Estado de Mato Grosso do Sul, Brasil, levaram a investigações entomológicas na área, com o objetivo de identificar a população de flebotomíneo vetora. MATERIAL E MÉTODO: A pesquisa foi realizada no peri e intradomicílio de três residências urbanas e em ecótopo natural, representado por uma gruta, situado fora do perímetro urbano. As capturas, semanais em sua maioria, foram realizadas com armadilhas automáticas luminosas, no período de 1984 a 1986. Os dados metereológicos desse período foram obtidos junto à Estação Metereológica da cidade e os de 1925 a 1982, de bibliografia. RESULTADOS: A fauna flebotomínea urbana, composta de oito espécies, mostrou-se semelhante à da gruta, porém nesta, a abundância das espécies foi maior. Na área urbana, Lu. cruzi predominou tanto no intra como no peridomicílio: no bairro central, representou 90,3% dos espécimens e nos dois bairros periféricos, os seus percentuais foram menores. Lu. forattinii, também, teve freqüência expressiva em um dos bairros periféricos (39,0 %. Na gruta, Lu. corumbaensis foi a espécie predominante. Comenta-se o impacto das condições climáticas e do inseticida aplicado na área urbana na freqüência das espécies, e da utilização da gruta como criadouro pelos flebotomíneos, com base na evolução da razão entre os sexos ao longo do período. Foram adicionadas informações sobre antropofilia e de coleta com isca canina de Lu. forattinii. CONCLUSÃO: O predomínio de Lu. cruzi na área urbana; a expressiva freqüência de Lu. forattinii na periferia da cidade, bem como a sua antropofilia e o estreito grau de parentesco destas esp

  20. Notas sobre el problema habitacional en 'El otro sendero'

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    1988-01-01

    Full Text Available L'article réunit des notes de lecture sur le livre 'El Otro Sendero', à propos de la construction de logements pour les secteurs à bas revenus. L'importance de ce livre pour le public national est reconnue au départ et l'on tente d'étudier ses propositions en ce qui concerne le logement (chapitre 2. Après avoir examiné les propositions du texte, on commente quelques erreurs dans l'analyse des faits. Le commentaire final dit: La profusion d'analyses erronées qui visent à mettre en évidence la 'longue marche vers la propriété privée', comme le manque d'analyses spécifiques sur le problème du logement et sur celui de la ville -qui constituent le contexte des informels dans les barriadas-, nous font penser que nous sommes confrontés à un schéma idéologique a priori plus qu'à une étude de la réalité urbaine. El artículo proporciona notas de lectura del libro 'El Otro Sendero' referentes a la producción de vivienda para los sectores de bajos ingresos. Se parte de reconocer la importancia que ha tenido este libro en la audiencia nacional y se intenta estudiar sus proposiciones sobre la vivienda (capítulo 2. Luego de examinar las propuestas presentes en el texto, se comentan algunas incorrecciones en el análisis de los hechos. El comentario final dice: La profusión de análisis erróneos que apuntan a destacar la 'larga marcha hacia la propiedad privada', así como la falta de análisis específicos sobre el problema de la vivienda y el de la ciudad -que constituyen el contexto de los informales en las barriadas-, nos hacen pensar que estamos frente a un esquema ideológico a priori más que frente a un estudio de la realidad urbana. The article consists of interpretative notes on the book 'El Otro Sendero', which refer to the production of housing for low-income sectors. It begins by recognizing the importance this book has had for the national audience, and proposes to examine its propositions about housing (chapter 2. An

  1. Excessive visceral fat accumulation in advanced chronic obstructive pulmonary disease

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    Furutate R

    2011-08-01

    Full Text Available Ryuko Furutate1, Takeo Ishii1,2, Ritsuko Wakabayashi1, Takashi Motegi1,2, Kouichi Yamada1,2, Akihiko Gemma2, Kozui Kida1,21Respiratory Care Clinic, Nippon Medical School, Kudan-Minami, Chiyoda-ku, Tokyo, Japan; 2Department of Internal Medicine, Division of Pulmonary Medicine, Infectious Diseases and Oncology, Nippon Medical School, Sendagi, Bunkyo-ku, Tokyo, JapanBackground: Previous studies have suggested links between chronic obstructive pulmonary disease (COPD, cardiovascular disease, and abdominal obesity. Although abdominal visceral fat is thought to be associated with cardiovascular risk factors, the degree of visceral fat accumulation in patients with COPD has not been directly studied. The aim of this study was to investigate the abdominal visceral fat accumulation and the association between visceral fat and the severity and changes in emphysema in COPD patients.Methods: We performed clinical and laboratory tests, including pulmonary function, dyspnea score, and the six-minute walking test in COPD patients (n = 101 and control, which included subjects with a smoking history but without airflow obstruction (n = 62. We used computed tomography to evaluate the abdominal visceral fat area (VFA, subcutaneous fat area (SFA, and the extent of emphysema.Results: The COPD group had a larger VFA than the control group. The prevalence of non-obese subjects with an increased VFA was greater in the Global Initiative for Chronic Obstructive Lung Disease Stages III and IV than in the other stages of COPD. The extent of emphysema was inversely correlated with waist circumference and SFA. However, VFA did not decrease with the severity of emphysema. VFA was positively correlated with the degree of dyspnea.Conclusion: COPD patients have excessive visceral fat, which is retained in patients with more advanced stages of COPD or severe emphysema despite the absence of obesity.Keywords: abdominal obesity, chronic obstructive pulmonary disease, emphysema

  2. Notas sobre la historia de la garza morena ardea cocoi (aves en Colombia

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    Cruz Millán Carlos A.

    1982-03-01

    Full Text Available

    Una revisión de la bibliografía sobre esta especie, demuestra que si bien existe una serie de notas relacionadas con su distribución geográfica, prácticamente nada se ha escrito en relación con sus costumbres, fuera claro está, de comentarios muy generales consignados en manuales sobre faunas regionales.

  3. Consequências da ingestão moderada de etanol na discriminação de notas musicais

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    Jandilson Avelino da Silva

    2015-03-01

    Full Text Available O objetivo deste estudo foi avaliar se existe relação entre a ingestão moderada de etanol e a discriminação das notas musicais RÉ, FÁ, e LÁ, em adultos jovens. Essas notas apresentam frequências fundamentais respectivamente de 293,665 Hz, 349,228 Hz e 440 Hz, de temperamento igual, escolhidas devido estarem em localização central na escala musical ocidental padrão. Participaram do estudo 40 voluntários de 18 a 29 anos, não músicos, saudáveis, não usuários de fármacos ou outras substâncias tóxicas, exceto o etanol de forma moderada e aguda. Por meio do software"Psysounds", criado especificamente para esse estudo, esses voluntários tiveram de escolher, entre duas notas musicais, uma das notas teste apresentada inicialmente. Os dados foram tratados com um teste X2 de aderência para comparar as frequências de acertos das notas musicais de teste nas condições de ingestão e de não ingestão de álcool. As análises mostraram diferenças significantes nas frequências de acertos para todas as notas utilizadas, sugerindo que a ingestão de etanol prejudica a discriminação das notas musicais RÉ, FÁ e LÁ.

  4. Cost of visceral leishmaniasis care in Brazil.

    Science.gov (United States)

    de Carvalho, Isis Polianna Silva Ferreira; Peixoto, Henry Maia; Romero, Gustavo Adolfo Sierra; de Oliveira, Maria Regina Fernandes

    2017-12-01

    To estimate the Brazilian direct and indirect costs of human visceral leishmaniasis (VL) in 2014. Cost-of-illness study on the Brazilian public health system and societal perspective. VL cases registered in the Notifiable Diseases Information System in the year of 2014 were considered. Direct medical costs regarding diagnostic, treatment and care provided to patients with VL were estimated through the top-down approach. The indirect costs related to productivity loss due to premature mortality and morbidity were estimated by means of the human-capital method. In 2014, 9895 suspected cases of VL were reported in the Notifiable Diseases Information System, and 3453 were later confirmed. There were 234 patients with Leishmania-HIV coinfection underwent a secondary prophylaxis. The total cost of VL in Brazil was US$ 14 190 701.50 (US$ 14 189 150.10 to 14 199 940.53) that varied according to the sensitivity analysis. The total of direct medical costs corresponded to US$ 1 873 681.96 (US$1 872 130.55 to 1 882 920.99), and the majority of costs was associated with hospitalisation (40%), followed by treatment (22%), and secondary prophylaxis (18%). Productivity loss corresponded to US$ 11 421 683.37 for premature mortality and US$ 895 336.18 for work absence due to hospitalisation by the illness. VL represents an expensive health problem for the Brazilian public health system and society, mainly because of its productivity loss due to premature mortality. Interventions to reduce VL lethality could have a great impact on decreasing the cost of illness. © 2017 John Wiley & Sons Ltd.

  5. Human visceral leishmaniasis: a picture from Italy.

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    Abdalmaula, Giuma Harun; Barbadoro, Pamela; Marigliano, Anna; Illuminati, Diego; Di Stanislao, Francesco; D'Errico, Marcello Mario; Prospero, Emilia

    2013-12-01

    The aim of our study was to describe the distribution of Visceral Leishmaniasis (VL) in Italy, focusing on HIV-infected patients, to estimate the burden of the disease and the public health actions that should be undertaken. A review of official notifications and hospitalization data has been performed. From 2006 to 2008, a total of 289 cases of VL were notified; the overall notification rate was 1.63/1,000,000 (95% CI 1.45-1.83). In total, 1192 VL-associated hospitalizations were detected, with a hospitalization rate of 6.71/1,000,000 (95% CI 6.34-7.10). For the age group "≤ 24 years", a statistically significant increase was detected (p<0.05). A total of 68.9% (n = 821) of hospitalizations were detected in HIV-positive patients. The geographic distribution of rates revealed a significant increase in the north-eastern area of the country. Our study confirms that the epidemiological pattern of VL is changing and that, in Italy, control measures and preventive strategies should be based on not only the official notification system but also hospital data. This would lead to the identification of areas of parasite spread and to the creation of awareness campaigns geared toward general practitioners in the affected areas. Easy case detection would allow for timely public health actions and strategies for the implementation of more effective interventions for reservoir control. Copyright © 2013 King Saud Bin Abdulaziz University for Health Sciences. Published by Elsevier Ltd. All rights reserved.

  6. Making Climate Change Visceral Through the Arts

    Science.gov (United States)

    Bilodeau, C.

    2016-12-01

    Through their affective power, the arts offer a more visceral understanding of our global crisis and have a greater potential to inspire people to take action than scientific data alone. In this talk, I will look at three projects that use art to translate scientific data into sensory experiences, galvanize communities around visions of a positive future, and make climate change relevant to our lives. Jill Pelto's work makes science visible. A recent graduate from the University of Maine, Pelto practices what she calls glaciogenic art. As an artist and scientist, she uses her creative skills to communicate information about extreme environmental issues. Pelto's watercolors merge scientific data commonly found on graphs with the interpretation of that data in the form of illustrations. The result is an immediate understanding of the science and its implications. The Land Art Generator Initiative provides a platform for artists, architects, landscape architects, and other creatives working with engineers and scientists to bring forward human-centered solutions for sustainable energy infrastructures that enhance the city as works of public art while cleanly powering thousands of homes. Land Art Generator works are optimistic reminders that there is still time to make positive changes. Climate Change Theatre Action was a series of 100 readings and performances of climate change plays, poems and songs, written by writers from all six continents, presented in over 25 countries in support of the United Nations 2015 Paris Climate Conference. Events ranged from informal readings in classrooms to fully-staged performances, and often included presentations and/or panel conversations with scientists. The project reached people from all walks of life (including homeless youth and refugees) and had a powerful impact on audiences.

  7. Elective visceral hybrid repair of type III thoracoabdominal aortic aneurysm

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    Marjanović Ivan

    2012-01-01

    Full Text Available Introduction. According to the classification given by Crawford et al. type III thoracoabdominal aortic aneurysm (TAAA is dilatation of the aorta from the level of the rib 6 to the separation of the aorta below the renal arteries, capturing all the visceral branch of aorta. Visceral hybrid reconstruction of TAAA is a procedure developed in recent years in the world, which involves a combination of conventional, open and endovascular aortic reconstruction surgery at the level of separation of the left subclavian artery to the level of visceral branches of aorta. Case report. We presented a 75-years-old man, with elective visceral hybrid reconstruction of type III TAAA. Computerized scanning (CT angiography of the patient showed type III TAAA with the maximum transverse diameter of aneurysm of 92 mm. Aneurysm started at the level of the sixth rib, and the end of the aneurysm was 1 cm distal to the level of renal arteries. Aneurysm compressed the esophagus, causing the patient difficulty in swallowing act, especially solid food, and frequent back pain. From the other comorbidity, the patient had been treated for a long time, due to chronic obstructive pulmonary disease and hypertension. In general endotracheal anesthesia with epidural analgesia, the patient underwent visceral hybrid reconstruction of TAAA, which combines classic, open vascular surgery and endovascular procedures. Classic vascular surgery is visceral reconstruction using by-pass procedure from the distal, normal aorta to all visceral branches: celiac trunk, superior mesenteric artery and both renal arteries, with ligature of all arteries very close to the aorta. After that, by synchronous endovascular technique a complete aneurysmal exclusion of thoracoabdominal aneurysm with thoracic stent-graft was performed. The postoperative course was conducted properly and the patient left the Clinic for Vascular Surgery on postoperative day 21. Control CT, performed 3 months after the surgery

  8. Avaliação das ações de controle da leishmaniose visceral (calazar em uma área endêmica do Estado da Bahia, Brasil (1995-2000 Evaluation of control measures for visceral leishmaniasis (kala azar in an endemic area in Bahia, Brazil (1995-2000

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    Simone Souza de Oliveira

    2003-12-01

    Full Text Available Este estudo objetivou avaliar as ações do Programa de Controle da Leishmaniose Visceral no Município de Feira de Santana, Bahia, Brasil. Foram coletadas informações das ações no período de 1995 a junho de 2000, considerando-se a periodicidade e cobertura, e a evolução da incidência nas áreas trabalhadas. A variável dependente foi a incidência de casos humanos e as variáveis independentes foram: percentual de prédios trabalhados para inquérito canino e prédios borrifados, número de ciclos de inquérito canino e borrifação e prevalência canina. Para avaliar a correlação entre as ações e a incidência de casos humanos calculou-se os coeficientes de correlação de Pearson e procedeu-se à analise de regressão linear. Não foi observada associação entre a prevalência de cães com sorologia positiva e a incidência de casos humanos. As variáveis que permaneceram no modelo final e que tiveram efetividade mais destacada no controle da doença foram: percentual de prédios trabalhados para inquérito e borrifação e número de ciclos de inquérito canino, capazes de explicar 40% da incidência de casos humanos. Os achados constituem material relevante para discussão da efetividade das ações de controle da leishmaniose visceral.This study evaluated the Control Program for Visceral Leishmaniasis in the city of Feira de Santana, Bahia, Brazil. Information was collected on control measures from 1995 to June 2000, and focused on periodicity, coverage, and incidence trends in the target areas. The dependent variable was the incidence rate for human cases, and independent variables were: percentage of buildings visited in the canine survey and buildings sprayed, number of cycles in the canine survey and spraying, and canine prevalence rate. To evaluate the correlation between control measures and the human visceral leishmaniasis incidence rate, we calculated the Pearson’s correlations coefficients and performed linear

  9. One-step radiosynthesis of {sup 18}F-AlF-NOTA-RGD{sub 2} for tumor angiogenesis PET imaging

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Shuanglong; Liu, Hongguang; Xu, Yingding; Cheng, Zhen [Stanford University, Molecular Imaging Program at Stanford (MIPS), Canary Center at Stanford for Cancer Early Detection, Bio-X Program, Department of Radiology, Stanford, CA (United States); Jiang, Han [Stanford University, Molecular Imaging Program at Stanford (MIPS), Canary Center at Stanford for Cancer Early Detection, Bio-X Program, Department of Radiology, Stanford, CA (United States); Institute of Nuclear Medicine and Molecular Imaging, and the Second Affiliated Hospital of Zhejiang University School of Medicine, Key Laboratory of Medical Molecular Imaging of Zhejiang Province, Department of Nuclear Medicine, Medical PET Center, Hangzhou, Zhejiang (China); Zhang, Hong [Institute of Nuclear Medicine and Molecular Imaging, and the Second Affiliated Hospital of Zhejiang University School of Medicine, Key Laboratory of Medical Molecular Imaging of Zhejiang Province, Department of Nuclear Medicine, Medical PET Center, Hangzhou, Zhejiang (China)

    2011-09-15

    One of the major obstacles of the clinical translation of {sup 18}F-labeled arginine-glycine-aspartic acid (RGD) peptides has been the laborious multistep radiosynthesis. In order to facilitate the application of RGD-based positron emission tomography (PET) probes in the clinical setting we investigated in this study the feasibility of using the chelation reaction between Al{sup 18}F and a macrocyclic chelator-conjugated dimeric RGD peptide as a simple one-step {sup 18}F labeling strategy for development of a PET probe for tumor angiogenesis imaging. Dimeric cyclic peptide E[c(RGDyK)]{sub 2} (RGD{sub 2}) was first conjugated with a macrocyclic chelator, 1,4,7-triazacyclononane-1,4,7-triacetic acid (NOTA), and the resulting bioconjugate NOTA-RGD{sub 2} was then radiofluorinated via Al{sup 18}F intermediate to synthesize {sup 18}F-AlF-NOTA-RGD{sub 2}. Integrin binding affinities of the peptides were assessed by a U87MG cell-based receptor binding assay using {sup 125}I-echistatin as the radioligand. The tumor targeting efficacy and in vivo profile of {sup 18}F-AlF-NOTA-RGD{sub 2} were further evaluated in a subcutaneous U87MG glioblastoma xenograft model by microPET and biodistribution. NOTA-RGD{sub 2} was successfully {sup 18}F-fluorinated with good yield within 40 min using the Al{sup 18}F intermediate. The IC{sub 50} of {sup 19}F-AlF-NOTA-RGD{sub 2} was determined to be 46 {+-} 4.4 nM. Quantitative microPET studies demonstrated that {sup 18}F-AlF-NOTA-RGD{sub 2} showed high tumor uptake, fast clearance from the body, and good tumor to normal organ ratios. NOTA-RGD{sub 2} bioconjugate has been successfully prepared and labeled with Al{sup 18}F in one single step of radiosynthesis. The favorable in vivo performance and the short radiosynthetic route of {sup 18}F-AlF-NOTA-RGD{sub 2} warrant further optimization of the probe and the radiofluorination strategy to accelerate the clinical translation of {sup 18}F-labeled RGD peptides. (orig.)

  10. AVALIAÇÃO DE INICIADORES E PROTOCOLO PARA O DIAGNÓSTICO DA PANCITOPENIA TROPICAL CANINA POR PCR

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    Daniel C. L. Linhares

    2006-10-01

    Full Text Available Este trabalho foi desenvolvido com o objetivo de padronizar um protocolo para a reação em cadeia da polimerase (PCR e selecionar oligonucleotídeos iniciadores para a detecção específica de Ehrlichia canis, em uma única etapa de reação. Inicialmente foram obtidas seqüências depositadas no Genbank, referentes ao gene que codifica o 16S rRNA das espécies E. canis (número de acesso = AF162860, E. ewingii (U96436, E. platys (AF1567844, E. chaffeensis, (U86665, E. phagocytophila genogrupo (U02521, E. bovis (AF294789 e E. risticii (M21290, as quais foram submetidas ao alinhamento genético para a construção dos iniciadores. Do alinhamento foi selecionado, a partir de uma região semiconservada, um iniciador específico para E. canis, designado EBR1 (5’-cctctggctataggaaattg- 3’ e, de uma região conservada, um iniciador genérico EBR5 (5’-ggagtgcttaacgcgttag- 3’. Paralelamente foram obtidas amostras de sangue de dez cães que apresentavam infecção aguda por E. canis, confirmado pela presença de mórulas intracitoplasmáticas, características da riquétsia, em células mononucleares sangüíneas. O DNA genômico extraído dessas amostras foi utilizado para a avaliação da reação de PCR, empregando-se um protocolo adaptado de outros autores e o par de oligos EBR1/EBR5, selecionado neste trabalho. A reação de PCR apresentou resultados positivos para os 10 isolados de E. canis, amplificando o fragmento esperado de 765 pares de bases do gene 16S rRNA. Resultados negativos verificados nas reações de PCR para amostras de DNA genômico de Babesia canis, Hepatozoon canis, Haemobartonella sp., Trypanosoma evansi e do hospedeiro (Canis familiaris livre de infecção indicaram a segurança do método quanto à especificidade para a discriminação de E. canis. PALAVRAS-CHAVE: Cães, Ehrlichia canis, erliquiose canina, hemoparasitose, reação em cadeia da polimerase

  11. Biofabrication of zinc oxide nanoparticles using fruit extract of Rosa canina and their toxic potential against bacteria: A mechanistic approach

    Energy Technology Data Exchange (ETDEWEB)

    Jafarirad, Saeed; Mehrabi, Meysam [Research Institute for Fundamental Sciences (RIFS), University of Tabriz, Tabriz (Iran, Islamic Republic of); Divband, Baharak [Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz (Iran, Islamic Republic of); Inorganic Chemistry Department, Faculty of Chemistry, University of Tabriz, Tabriz (Iran, Islamic Republic of); Kosari-Nasab, Morteza [Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz (Iran, Islamic Republic of)

    2016-02-01

    The use of plant extract in the biosynthesis of nanoparticles (NPs) can be an eco-friendly approach and have been suggested as a possible alternative to classic methods namely physical and chemical procedures. In this study, the biosynthesis of zinc oxide (ZnO) NPs by both “conventional heating” (CH) and “microwave irradiation” (MI) methods has been reported. Stable and spherical ZnONPs were produced using zinc nitrate and flesh extract of Rosa canina fruit (rosehip) which was used as a precursor. The flesh extract acts as a reducing and capping agent for generation of ZnONPs. The structural, morphological and colloidal properties of the as-synthesized NPs have been confirmed by X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM), Energy Dispersive X-ray (EDX), Fourier transform Infrared (FT-IR) and Dynamic Light Scattering (DLS). In comparison with the CH method, the MI method has some advantages such as significantly short reaction time (within 8 min) owing to the high heating rate and thus the accelerated reaction rate. Both methods led to the synthesis of nearly identical NPs with respect to shape and size according to the results of DLS, XRD and SEM techniques. The possible mechanism for synthesis pathway has been proposed based on FT IR results, XRD patterns, potentiometric data and antioxidant activity. In addition, the antibacterial activity of as-prepared ZnONPs was investigated against several bacteria such as Listeria monocytogenes, Escherichia coli, Salmonella typhimurium. Moreover, the efficacy of ZnONPs to treat cancer cell lines were measured by means of cell viability test via MTT assay in which concentrations of 0.05 and 0.1 mg/mL of ZnONPs induced a very low toxicity. Thus, the present investigation reveals that ZnONPs have the potential for various medical and industrial applications. - Highlights: • We report a green producing route from biomaterials to potentially biomedical applications. • We comprise two methods in respect

  12. Estudo comparativo dos métodos diagnósticos para Leishmaniose Visceral em cães oriundos de Ilha Solteira, SP Comparative study of diagnostic methods for visceral leishmaniasis in dogs from Ilha Solteira, SP

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    Juliana de Assis

    2010-03-01

    Full Text Available O objetivo da presente pesquisa foi avaliar comparativamente os métodos diagnósticos da Leishmaniose Visceral Canina (LVC, utilizando-se o ensaio imunoenzimático indireto (ELISA, a reação de imunofluorescência indireta (RIFI, a histoquímica (HE e a imunoistoquímica (IMIQ em tecidos de órgãos, como o baço, linfonodo e fígado. Além disso, a Reação em Cadeia pela Polimerase (PCR das amostras de sangue e dos tecidos foi utilizada para comparar e confirmar os diagnósticos negativos e não conclusivos pelos métodos acima. Para esse estudo, foram utilizados 34 cães com diferentes sintomas da LVC, classificados em polissintomáticos, oligossintomáticos e assintomáticos e eutanasiados no Centro de Controle de Zoonoses (CCZ de Ilha Solteira, SP. Os índices de positividade para os testes ELISA, IMIQ, RIFI e HE foram de 65,0, 62,0, 56,0 e 56,0%, respectivamente, sendo a maior positividade detectada nos cães polissintomáticos (92,0%, seguida pelos oligossintomáticos (57,0% e assintomáticos (12,5%. A PCR confirmou os resultados positivos pelas outras técnicas e ainda detectou DNA do parasita nos tecidos de 100% dos cães negativos e em 89,0% dos suspeitos, elevando para 97,0% a positividade. Em conclusão, a PCR demonstrou ser o método mais sensível e preciso para o diagnóstico definitivo da LVC.The purpose of the present work was a comparative study of diagnostic methods for Canine Visceral Leishmaniasis (CVL using serological methods, enzyme-linked immunosorbent assay (ELISA and indirect fluorescent antibody test (IFAT, histochemical (HE and immunohistochemical (IMHC tests using spleen, lymph node and liver canine tissues. In addition, Polymerase Chain Reaction (PCR was done in blood and in tissues in order to compare and confirm no conclusive and negative diagnosis by the methods above. For this study, 34 dogs were divided according to clinical signs in asymptomatic, oligosymptomatic and polisymptomatic Leishmania-infected dogs

  13. Mulheres e homens: diferentes também na percepção das notas musicais?

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    Jandilson Avelino da Silva

    Full Text Available O objetivo deste estudo foi avaliar a percepção das notas musicais RÉ, FÁ, e LÁ em adultos jovens. Participaram do estudo 20 homens e 20 mulheres de 18 a 29 anos, saudáveis, não músicos e não usuários de fármacos ou outras substâncias tóxicas. Realizou-se um teste de discriminação de notas musicais com escolha forçada entre duas alternativas, por meio do qual os participantes escolheram um estímulo previamente apresentado. Comparou-se a frequência de acertos na discriminação das notas musicais em relação ao sexo dos participantes. Encontraram-se diferenças estatisticamente significantes na nota musical Fá, sendo que os homens apresentaram maior quantidade de acertos que as mulheres. Esses resultados encontrados sugerem diferença na percepção das notas musicais para mulheres e homens.

  14. The Visceral Novel Reader and Novelized Medicine in Georgian Britain.

    Science.gov (United States)

    Class, Monika

    The article introduces "the visceral novel reader" as a diachronic, context-sensitive mode of novelistic reception, in which fact and fiction overlap cognitively: the mental rehearsal of the activity of seeing, hearing, smelling, tasting, and touching while reading novels and, vice versa, the mental rehearsal of novels in the act of perceiving the real world. Located at the intersection of literature, medicine and science, "the visceral novel reader" enhances our understanding of the role that novels played in the dialectic construction of erudition in English. In Georgian Britain, reading practices became a testing ground for the professionalization of physicians, natural philosophers, and men of letters. While it was in the professionals' common interest to implement protocols that taught readers to separate body from mind, and fact from fiction, novels came to stand for "debased" (visceral) reading. Novels inverted these notions by means of medicalization (regimentation, somatization, and individuation) and contributed to the professional stratification of medicine and literature.

  15. VISCERAL ABDOMINAL PAIN AND OPPORTUNITIES OF SPASMOLYTIC TREATMENT

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    E.A. Kornienko

    2008-01-01

    Full Text Available Results of treatment of 30 children with visceral abdominal pain caused by different etiological factors with neurotropic selective m9cholinergic antagonist hyoscine butilbromide (buscopan are presented in this article. Two groups of children were treated with hyoscine butilbromide and drotaverine accordingly. Administration of hyoscine butilbromide allows to stop pain in 93% of patients; mean duration of abdominal pain was 3,4 ± 1,2 days (4,2 ± 1,4 days in children treated with drotaverine, р < 0,05. Activity of dyspeptic disorders was decreased at the time of treatment. a tolerance to hyoscine butilbromide was satisfactory, and no adverse events were registered. hyoscine butilbromide is effective in treatment of visceral abdominal pain in children, allowing shortening its duration more actively then drotaverine.Key words: children, visceral abdominal pain, hyoscine butilbromide.

  16. Tuberous sclerosis with visceral leishmaniasis: a case report

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    Pandey Krishna

    2009-09-01

    Full Text Available Abstract Introduction Visceral leishmaniasis, a tropical infectious disease, is a major public health problem in India. Tuberous sclerosis, a congenital neuro-ectodermosis, is an uncommon disease which requires life long treatment. Case presentation A 15-year-old Indian patient, presented to the outpatient department of our institute with a high-grade fever for two months, splenomegaly and a history of generalized tonic-clonic convulsions since childhood. The clinical and laboratory findings suggested visceral leishmaniasis with tuberous sclerosis. The patient was treated with miltefosine and antiepileptics. Conclusion The patient responded well and in a follow up six months after presentation, she was found free of visceral leishmaniasis and seizures. Diagnosis and treatment of this rare combination of diseases is difficult.

  17. Visceral metabolism and efficiency of energy use by ruminants

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    Kozloski Gilberto Vilmar

    2001-01-01

    Full Text Available The visceral system (liver and portal-drained viscera represents an interface between diet and the animal, and it acts as the main site of regulation of nutrients that are used for maintenance, growth, lactation, reproduction, and physical activities of animals. However the functions carried out by visceral organs have, however, a significant energetic cost and are influenced by a variety of factors, such as the level of feed intake and diet composition, among others. As a result, variable quantities of substances are metabolized by them and, thus, the pattern and the quantity of nutrients available to the peripheral tissues can be quite different from those absorbed at the intestinal lumen. Probably, the major source of variation in the efficiency of utilization of metabolizable energy among feeds is associated mainly with visceral metabolism and it is unlikely that the ratio ketogenic/glucogenic of absorbed substances has determinant effect under physiological conditions.

  18. The role of rose hip (Rosa canina L powder in alleviating arthritis pain and inflammation – part II animal and human studies

    Directory of Open Access Journals (Sweden)

    Marstr

    2016-05-01

    Full Text Available Kristian Marstrand,1 Joan Campbell-Tofte2 1Department of Orthopaedic Surgery, Elverum Hospital, Elverum, Norway; 2Coordinating Research Unit, Frederiksberg University Hospital, Frederiksberg, Copenhagen, Denmark Abstract: Rosa canina fruits (often known as rose hip have been used in herbal remedies since ancient times. On the basis of anecdotal reports about the effects of rose hip preparations on human health and citations to the same in ancient texts, in vitro and in vivo studies have been conducted that have made it possible to learn about some active ingredients in rose hip, as well as how the component compounds might exert their effects. From such studies, it has been documented that there is a great variation in active ingredients when comparing different rose hip products. Factors which affect the quality and quantity of active ingredients in the rose hip products include the subspecies of the particular R. canina from which the fruits are harvested, the environment during plant growth, time of harvesting, and the amount of seeds and shells/husks incorporated into the preparation. Studies in animals and in humans are of great importance for determining the true bio-effects of rose hip. Such in vivo studies that have only been performed during the last 2 decades indicate that the treatment of patients with different types of joint disease with rose hip powder based on a subspecies of R. canina (Lito containing a certain galactolipid (GOPO can alleviate pain, improve daily activity, and even reduce the consumption of regular pain relievers. As several rose hip components have been shown to be anti-inflammatory, it is suggested that anti-inflammatory mechanisms might explain some reductions in symptoms associated with administering rose hip to arthritic conditions. Although the number of publications of randomized and placebo-controlled clinic studies with rose hip is small, the overriding evidence is that the anti-inflammatory effects from

  19. Série temporal da leishmaniose visceral em Aracaju, estado de Sergipe, Brasil (1999 a 2008: aspectos humanos e caninos Time series of visceral leishmaniasis in Aracaju, state of Sergipe, Brazil (1999 to 2008: human and canine aspects

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    Marco Aurélio de Oliveira Góes

    2012-06-01

    Full Text Available INTRODUÇÃO: Considerada doença negligenciada pela OMS, a Leishmaniose visceral (LV tem se expandido e urbanizado, sendo sua transmissão e expansão associadas a diversos fatores. OBJETIVO: Avaliar aspectos epidemiológicos da LV no município de Aracaju/SE, por meio de estudo retrospectivo da série histórica de LV humana e canina no período de 1999-2008. MÉTODOS: Foram utilizados dados secundários do SINAN para os casos humanos, e o resultado dos inquéritos caninos e atendimentos da demanda passiva do Centro de Controle de Zoonoses (CCZ. RESULTADOS: Foram notificados 192 casos autóctones de LV humana, sendo 63,5% do gênero masculino. Crianças entre 1 e 4 anos foram mais acometidas (29,2%, seguidas de adultos entre 20-29 anos (15,6% e de crianças entre 5-9 anos (15,1%. A letalidade geral foi de 8,9%, sendo mais acentuada em pessoas entre 60 a 69 anos (60%; 32,3% dos casos autóctones realizaram sorologia para HIV, com positividade de 6,9%. A confirmação laboratorial foi realizada principalmente mediante a imunofluorescência indireta isolada (71,1%. Dos 58.161 cães que realizaram sorologia a positividade foi de 5,4%, sendo 87,0% dos inquéritos realizados anualmente pelo CCZ, com uma positividade de 4,4%. Dos 7.501 cães trazidos ao CCZ por diversas queixas, o exame sorológico foi reagente em 12,0%. CONCLUSÃO: Os dados denotam o caráter endêmico da LV no município, mostrando a necessidade de ações que permitam a diminuição do risco para a população, principalmente aquela onde a incidência e a letalidade são maiores, como melhorias no diagnóstico da LV, assim como na co-infecção com HIV e no monitoramento da população canina, entre outros.INTRODUCTION: Considered as a neglected disease by the WHO, visceral leishmaniasis (VL has expanded and urbanized. Its transmission and expansion have been linked to several factors. OBJECTIVE: To evaluate the epidemiological aspects of VL in the city of Aracaju/SE, through

  20. Epidemiology of visceral leishmaniasis in Georgia.

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    Giorgi Babuadze

    2014-03-01

    Full Text Available This study investigated the transmission and prevalence of Leishmania parasite infection of humans in two foci of Visceral Leishmaniasis (VL in Georgia, the well known focus in Tbilisi in the East, and in Kutaisi, a new focus in the West of the country. The seroprevalence of canine leishmaniasis was investigated in order to understand the zoonotic transmission. Blood samples of 1575 dogs (stray and pet and 77 wild canids were tested for VL by Kalazar Detect rK39 rapid diagnostic tests. Three districts were investigated in Tbilisi and one in Kutaisi. The highest proportions of seropositive pet dogs were present in District #2 (28.1%, 82/292 and District #1 (26.9%, 24/89 in Tbilisi, compared to 17.3% (26/150 of pet dogs in Kutaisi. The percentage of seropositive stray dogs was also twice as high in Tbilisi (16.1%, n = 670 than in Kutaisi (8%, n = 50; only 2/58 wild animals screened were seropositive (2. 6%. A total of 873 Phlebotomine sand flies were collected, with 5 different species identified in Tbilisi and 3 species in Kutaisi; 2.3% of the females were positive for Leishmania parasites. The Leishmanin Skin Test (LST was performed on 981 human subjects in VL foci in urban areas in Tbilisi and Kutaisi. A particularly high prevalence of LST positives was observed in Tbilisi District #1 (22.2%, 37.5% and 19.5% for ages 5-9, 15-24 and 25-59, respectively; lower prevalence was observed in Kutaisi (0%, 3.2% and 5.2%, respectively; P<0.05. This study shows that Tbilisi is an active focus for leishmaniasis and that the infection prevalence is very high in dogs and in humans. Although exposure is as yet not as high in Kutaisi, this is a new VL focus. The overall situation in the country is alarming and new control measures are urgently needed.

  1. Management of visceral leishmaniasis with therapeutic vaccines

    Directory of Open Access Journals (Sweden)

    Rawat K

    2016-09-01

    Full Text Available Keerti Rawat,1 Narendra K Yadav,1 Sumit Joshi,1 Sneha Ratnapriya,1 Amogh A Sahasrabuddhe,2 Anuradha Dube1 1Division of Parasitology, 2Division of Molecular and Structural Biology, Council of Scientific and Industrial Research-Central Drug Research Institute, Lucknow, India Abstract: Visceral leishmaniasis (VL, a phlebotomine-borne neglected tropical disease, is caused by parasites of the Leishmania donovani complex. While L. donovani infection is restricted to the Indian subcontinent and East Africa, where transmission is anthroponotic, Leishmania infantum occurs in Europe, North Africa, and parts of Latin America, where it is zoonotic in nature with dogs as reservoir hosts. Though the incidence of VL caused by L. infantum has been on the decline, L. donovani continues to cause epidemics periodically. By and large, a small proportion of L. donovani infection manifests as clinical disease but majority of the infected individuals remain asymptomatic and contribute to the perpetuation of the VL transmission cycle via the sand fly vector. This is one of the major stumbling blocks to World Health Organization initiatives to eliminate this deadly disease by 2020. These parasites reside within the host macrophages and impair the immune system of the infected individual, which ultimately results in marked immunosuppression. In the absence of any safe and effective vector control measure, attempts have been made to design therapeutic vaccine(s that can exclusively target infected macrophages. So far, two vaccines – a glycoprotein complex from L. donovani promastigote, fucose–mannose ligand with saponin, commercialized as Leishmune®, as well as a polyprotein vaccine formulation, Leish-111f + monophosphoryl lipid A plus squalene emulsion in combination with glucantime, have been successfully evaluated for their immunotherapeutic potential against canine VL. However, encouraging results obtained from several experimental trials so far against human VL

  2. Visceral schistosomiasis among domestic ruminants slaughtered in Wayanad, South India.

    Science.gov (United States)

    Ravindran, R; Lakshmanan, B; Ravishankar, C; Subramanian, H

    2007-11-01

    This short communication reports the prevalence of visceral schistosomiasis by worm counts from the mesentery of domestic ruminants of the hilly district of Wayanad, located in Kerala, one of the states in South India. We found 57.3, 50, and 4.7% of cattle, buffaloes and goats, respectively, had visceral schistosomiasis upon slaughter at a municipal slaughter house in Kalpetta. Our findings show that the prevalence of Schistosoma spindale infection is very high in Wayanad in comparison to previous reports from this and neighboring countries.

  3. [Visceral leishmaniasis, pemphigus and immunosuppressive treatment: case report from Morocco].

    Science.gov (United States)

    Maleb, A; Messaoudi, N; Chbouki, O; Daoudi, N; Oumghar, K; Lahmadi, K; Elmoussaoui, D; Ezzahraoui, K; Ngoh, Akwa E; Benomar, F; Abi, R; Jeaidi, A; Nazih, M; Belmekki, A; Chakour, M

    2011-02-01

    Atypical forms of visceral leishmaniasis associated with immunosuppressive treatment are difficult to diagnose and cause high mortality. The purpose of this report is to describe a case involving a 42-year-old patient living in a leishmaniasis-endemic area, who was undergoing immunosuppressive treatment using corticosteroids and methotrexate for pemphigus. Despite clinical and laboratory findings consistent with visceral leishmaniasis, detection of Leishmania bodies was a coincidental finding of cytological examination of bone marrow during workup for pancytopenia and associated clinical signs. This case argues in favor of systematic screening for this opportunistic parasitic disease before undertaking immunosuppressive treatment in patients presenting risk factors and consistent clinical/laboratory findings.

  4. Visceral leishmaniasis diagnosed in a patient with MALT lymphoma

    DEFF Research Database (Denmark)

    Kaae, Jeanette; Nørgaard, Peter; Himmelstrup, B

    2007-01-01

    We report a case of visceral leishmaniasis in a 66-year-old female with a history of MALT lymphoma in the gastrointestinal tract. The patient presented with major hemorrhage per rectum and perforation of the small intestine. Due to unexplained decreasing platelets, lymphoma bone marrow involvement...... was suspected and bone marrow examination was performed. Surprisingly, Leishman-Donovan bodies were detected. The low platelet count, caused by the combination of MALT lymphoma and visceral leishmaniasis, appears to have aggravated the symptoms of the intestinal lymphoma. Leishmaniasis should be suspected even...... among asymptomatic patients with immune compromising illnesses and a travel history to areas where leishmaniasis is endemic....

  5. Cutaneous and visceral leishmaniasis during anti-TNFα therapy.

    Science.gov (United States)

    Guarneri, Claudio; Bevelacqua, Valentina; Patterson, James W; Tchernev, Georgi

    2017-03-01

    The long-term use of novel antipsoriatic systemic biotechnological drugs may increase susceptibility to opportunistic infections. Several cases of visceral leishmaniasis have been reported in immunosuppressed individuals, including those who have been treated with tumour necrosis factor alpha (TNFα) blocking agents. Simultaneous occurrence of cutaneous and visceral involvement has been more rarely recorded in the medical literature. Herein, we describe a case of mucosal leishmaniasis occurring in a farmer living in an endemic region, who was treated with golimumab because of psoriatic arthritis. This highlights the importance of recognizing cutaneous lesions as a first indicator of possible underlying kala-azar disease.

  6. Functional dyspepsia: the role of visceral hypersensitivity in its pathogenesis.

    LENUS (Irish Health Repository)

    Keohane, John

    2012-02-03

    Functional, or non-ulcer, dyspepsia (FD) is one of the most common reasons for referral to gastroenterologists. It is associated with significant morbidity and impaired quality of life. Many authorities believe that functional dyspepsia and irritable bowel syndrome represent part of the spectrum of the same disease process. The pathophysiology of FD remains unclear but several theories have been proposed including visceral hypersensitivity, gastric motor dysfunction, Helicobacter pylori infection and psychosocial factors. In this review, we look at the evidence, to date, for the role of visceral hypersensitivity in the aetiology of FD.

  7. Presentation of AIDS with Disseminated Cutaneous and Visceral Leishmaniasis in Iran

    Directory of Open Access Journals (Sweden)

    Mohammadali Davarpanah

    2015-01-01

    Full Text Available Leishmaniasis is an infectious disease in form of visceral (VL, cutaneous (CL, and mucocutaneous (MCL leishmaniasis. Immunocompromised patients have increased risk of Leishmania infection, especially in endemic areas for visceral leishmaniasis, where in the world HIV/VL coinfection has become endemic. The case here suffers from both AIDS and visceral-cutaneous leishmaniasis. We report an Iranian woman with disseminated cutaneous and visceral leishmaniasis who became positive for HIV test.

  8. Abdominal diameters as indicators of visceral fat : comparison between magnetic resonance imaging and anthropometry

    NARCIS (Netherlands)

    van der Kooy, K; Leenen, R; Seidell, J C; Deurenberg, P.; Visser, M

    The aim of the present study was to investigate the usefulness of abdominal diameters to indicate visceral fat, their relationship with serum lipids and their capability of detecting changes in visceral fat. Before and after weight loss, visceral and subcutaneous fat, and the sagittal and transverse

  9. Quantitative analysis and comparison study of [18F]AlF-NOTA-PRGD2, [18F]FPPRGD2 and [68Ga]Ga-NOTA-PRGD2 using a reference tissue model.

    Directory of Open Access Journals (Sweden)

    Ning Guo

    Full Text Available With favorable pharmacokinetics and binding affinity for α(vβ(3 integrin, (18F-labeled dimeric cyclic RGD peptide ([(18F]FPPRGD2 has been intensively used as a PET imaging probe for lesion detection and therapy response monitoring. A recently introduced kit formulation method, which uses an (18F-fluoride-aluminum complex labeled RGD tracer ([(18F]AlF-NOTA-PRGD2, provides a strategy for simplifying the labeling procedure to facilitate clinical translation. Meanwhile, an easy-to-prepare (68Ga-labeled NOTA-PRGD2 has also been reported to have promising properties for imaging integrin α(vβ(3. The purpose of this study is to quantitatively compare the pharmacokinetic parameters of [(18F]FPPRGD2, [(18F]AlF-NOTA-PRGD2, and [(68Ga]Ga-NOTA-PRGD2. U87MG tumor-bearing mice underwent 60-min dynamic PET scans following the injection of three tracers. Kinetic parameters were calculated using Logan graphical analysis with reference tissue. Parametric maps were generated using voxel-level modeling. All three compounds showed high binding potential (Bp(ND = k(3/k(4 in tumor voxels. [(18F]AlF-NOTA-PRGD2 showed comparable Bp(ND value (3.75±0.65 with those of [(18F]FPPRGD2 (3.39±0.84 and [(68Ga]Ga-NOTA-PRGD2 (3.09±0.21 (p>0.05. Little difference was found in volume of distribution (V(T among these three RGD tracers in tumor, liver and muscle. Parametric maps showed similar kinetic parameters for all three tracers. We also demonstrated that the impact of non-specific binding could be eliminated in the kinetic analysis. Consequently, kinetic parameter estimation showed more comparable results among groups than static image analysis. In conclusion, [(18F]AlF-NOTA-PRGD2 and [(68Ga]Ga-NOTA-PRGD2 have comparable pharmacokinetics and quantitative parameters compared to those of [(18F]FPPRGD2. Despite the apparent difference in tumor uptake (%ID/g determined from static images and clearance pattern, the actual specific binding component extrapolated from kinetic

  10. Perfil clínico-laboratorial da erliquiose monocítica canina em cães de Salvador e região metropolitana, Bahia

    OpenAIRE

    Meneses, Íris Daniela Santos de; Souza, Bárbara Maria Paraná da Silva; Teixeira, Carla Maria Moreira; Guimarães, José Eugênio

    2008-01-01

    p. 770-776 Este trabalho foi realizado com o objetivo de traçar o perfil clínico e laboratorial para o diagnóstico da erliquiose canina. Utilizaram-se 75 cães, de ambos os sexos, diferentes idades e raças, da cidade de Salvador e região metropolitana, Bahia, incluídos na pesquisa a partir da suspeita clínica e por apresentar infestação de carrapatos ou histórico de exposição prévia ao vetor. Anticorpos anti- Ehrlichia canis foram encontrados em 98,66% (74/75) dos...

  11. Utilização de ferramentas de análise espacial na vigilância epidemiológica de leishmaniose visceral americana - Araçatuba, São Paulo, Brasil, 1998-1999 Use of spatial analysis tools in the epidemiological surveillance of American visceral leishmaniasis, Araçatuba, São Paulo, Brazil, 1998-1999

    Directory of Open Access Journals (Sweden)

    Vera Lucia Fonseca de Camargo-Neves

    2001-10-01

    Full Text Available O controle da Leishmaniose Visceral Americana (LVA está fundamentado no combate ao vetor e na eliminação do reservatório doméstico da área de foco - 200 metros em torno do caso humano ou canino. O presente trabalho tem como objetivo discutir a utilização de técnicas de análise espacial na vigilância epidemiológica da LVA no Município de Araçatuba, São Paulo, buscando estabelecer um modelo de vigilância epidemiológica em base territorial, redirecionando as estratégias de controle atualmente adotadas. Verificou-se que a transmissão da LVA não foi homogênea no município: a transmissão humana ocorreu nas áreas com maiores taxas de prevalência canina. A dispersão do vetor parece restrita a poucos domicílios, embora não tenha sido possível estabelecer um perfil da densidade vetorial. Visando estudar a distribuição do vetor e variáveis correlacionadas está sendo realizado estudo de campo por amostragem de domicílios, que permitirá o desenvolvimento de novas ferramentas de análise espacial e, possivelmente, permitirá redefinir as propostas de controle da endemia em ambiente urbano.The control of American visceral leishmaniasis (AVL is based on combating the vector and eliminating the domestic reservoir of the focus area - defined as 200 meters around human or canine cases. This paper discusses the use of spatial analysis techniques in the epidemiological surveillance of AVL in Araçatuba, São Paulo State, in order to propose a model for territorial epidemiological surveillance, reformulating current control strategies. The results showed that AVL transmission was not homogeneous; human cases were more frequent in areas with higher canine prevalence rates. Vector dispersion appeared to be restricted to a few houses, although it was not possible to model the vector density. In order to study the vector distribution and correlated covariates, a field study based on house sampling is being conducted. The results will

  12. Leishmania amazonensis in dog with clinical diagnosis of visceral leishmaniasis in Paraná State, Brazil – a case reportLeishmania amazonensis em cão com quadro clínico de leishmaniose visceral no Estado do Paraná, Brasil – relato de caso

    Directory of Open Access Journals (Sweden)

    Patrícia Mendes Pereira

    2012-02-01

    Full Text Available Leishmaniasis are antropozoonoses involving various species of Leishmania and hosts, with variable clinical presentations and considered of great importance to public health. This article describes a case of canine leishmaniasis caused by Leishmania amazonensis, from the town of Cambé, Paraná state, Brazil. This state is considered endemic for American cutaneous leishmaniasis (ACL in humans, but this is the first report in a dog in the region, caused by L. amazonensis. The taxonomic characterization of Leishmania was performed by multilocus enzyme electrophoresis technique (MLEE. Clinical signs were similar to those observed in visceral leishmaniasis as polyarthritis and progressive weight loss. It is concluded that the related polyarthritis when related to leishmaniasis, cannot be considered a clinical sign associated only with L. chagasi in view of the L. amazonensis also has this as one of its presentations. The LTA becomes a differential diagnosis of polyarthritis in dogs.As leishmanioses são antropozoonoses que envolvem diversas espécies de Leishmania e hospedeiros, tendo apresentações clínicas variáveis e consideradas de grande importância para a saúde pública. Este artigo descreve um caso de leishmaniose canina causada por Leishmania amazonensis, proveniente da cidade de Cambé, Paraná – Brasil. Este estado é considerado endêmico para a leishmaniose tegumentar americana (LTA em humanos, porém este é o primeiro relato em cão na região, provocado pela L. amazonensis. A caracterização taxonômica da Leishmania foi realizada através da técnica multilocus enzyme electrophoresis (MLEE. Os sinais clínicos foram similares aos observados na leishmaniose visceral como poliartrite e emagrecimento progressivo. Conclui-se que a poliartrite quando relacionada à leishmaniose não pode ser considerada um sinal clínico associado somente a L. chagasi, tendo em vista que a L. amazonensis também tem esta como uma das suas formas

  13. Vigilância de leishmaniose visceral americana em cães de área não endêmica, São Paulo Surveillance of American visceral leishmaniasis in dogs from a non-endemic area, Brazil

    Directory of Open Access Journals (Sweden)

    Elisa San Martin Mouriz Savani

    2003-04-01

    Full Text Available Realizou-se inquérito sorológico em cães domiciliados e errantes do Município de São José do Rio Preto, SP, para identificar animais infectados e detectar a possibilidade de transmissão da leishmaniose visceral americana. De novembro de 1998 a junho de 2000, 2.104 amostras de soros foram testadas por meio da reação de imunofluorescência indireta, empregando-se como antígeno formas promastigotas de Leishmania (L. chagasi. Observaram-se 2.092 amostras não reagentes e 12 reagentes. Dos cães com sorologia positiva foi possível realizar raspados de lesão em três animais. O material fixado em lâminas foi corado por Giemsa e, em apenas um, foram encontradas formas amastigotas características de Leishmania sp. Este resultado indica a necessidade de manutenção da vigilância sorológica canina e entomológica no município de São José do Rio Preto, a fim de detectar, precocemente, qualquer alteração na epidemiologia local.A serological survey among domestic and stray dogs of the municipality of São José do Rio Preto, Brazil, was conducted to identify infected animals and detect the chances of transmitting the American visceral leishmaniasis . From November 1998 to June 2000, 2,104 sera samples were tested by indirect immunofluorescence reaction, using as antigen promastigotes of Leishmania (L. chagasi. There were 2,092 no reactive samples and 12 reactive ones. Of the dogs with reactive serum it was possible to prepare lesion smears in only 3. The slide was stained by Giemsa method and only in one of them it was found amastigotes forms characteristic of Leishmania sp. This finding suggests the need of canine serological and entomological surveillance in São José do Rio Preto for an early detection of any local epidemiological changes.

  14. EVOLUCIÓN DE LA SOCIALIDAD, ESTRATEGIAS ALIMENTARIAS Y ANATOMÍA CRÁNEODENTAL EN LA SUBFAMILIA CANINAE Evolution of Sociality, Diet, and Craniodental Anatomy in the Subfamly Caninae

    Directory of Open Access Journals (Sweden)

    JOAO MUÑOZ-DURÁN

    2012-04-01

    organization of ancestral and modern mammal communities. Thus, it is important to identify what extinct carnivore species may have been social and to determine when, where and under which environmental contexts they evolved. The first step is to establish if there is an association between morphological traits, susceptible of being preserved in the fossil record, and differences in the degree of sociality of present day carnivores. The aim of this research was to establish if there is an association between variation in shape and variables related to mechanical properties of the skulls, and differences in sociality level, diet and sex in modern canids. The sample included 972 specimens of 33 canid species. We analyzed the shape of the lateral side of the cranium, zygomatic arch, dentary bone and mandibular corpus, as well as 20 variables related to mechanical properties of the skull. Results suggest that hypercarnivorous social canids have shapes and mechanical properties that are different from other modern canids. We found that morphological variation is not affected by sex. Results may be used to identify extinct Caninae species that were social and hypercarnivorous. Results also suggest that the evolution of sociality is dependent on phylogenetic relationships and ecological context, particularly the abundance of large prey and the presence of sympatric carnivores.

  15. Effect of a weight cycle on visceral fat accumulation

    NARCIS (Netherlands)

    van der Kooy, K; Leenen, R; Seidell, J C; Deurenberg, P.; Hautvast, J.G.A.J.

    1993-01-01

    Magnetic resonance imaging was used to study the effect of a single weight cycle on three fat depots: the visceral and subcutaneous abdominal depots and the subcutaneous depot at the trochanter level. Obese subjects (17 men, 15 women) were examined before and after weight-loss intervention and 67 wk

  16. Antimony to Cure Visceral Leishmaniasis Unresponsive to Liposomal Amphotericin B

    Science.gov (United States)

    Morizot, Gloria; Jouffroy, Romain; Faye, Albert; Chabert, Paul; Belhouari, Katia; Calin, Ruxandra; Charlier, Caroline; Miailhes, Patrick; Siriez, Jean-Yves; Mouri, Oussama; Yera, Hélène; Gilquin, Jacques; Tubiana, Roland; Lanternier, Fanny; Mamzer, Marie-France; Legendre, Christophe; Peyramond, Dominique; Caumes, Eric; Lortholary, Olivier; Buffet, Pierre

    2016-01-01

    We report on 4 patients (1 immunocompetent, 3 immunosuppressed) in whom visceral leishmaniasis had become unresponsive to (or had relapsed after) treatment with appropriate doses of liposomal amphotericin B. Under close follow-up, full courses of pentavalent antimony were administered without life-threatening adverse events and resulted in rapid and sustained clinical and parasitological cure. PMID:26735920

  17. visceral leishmaniasis with concomittant post kala-azar dermal ...

    African Journals Online (AJOL)

    hi-tech

    2003-08-08

    Aug 8, 2003 ... province, Kenya. INTRODUCTION. Visceral Leishmaniasis or kala-azar is caused by species of Leishmania donovani complex. These are Leishmania donovani donovani in East Africa and Asia, Leishmania donovani infantum in the Mediterranean Basin and. Leishmania donovani chagasi in Latin America ...

  18. [Uncommon cutaneous presentation of visceral Leishmaniasis associated with HIV].

    Science.gov (United States)

    Cossart, C; Le Moal, G; Garcia, M; Frouin, E; Hainaut-Wierzbicka, E; Roblot, F

    2016-12-01

    Visceral leishmaniasis is not normally expressed in skin. Herein, we describe the case of an HIV-positive patient who developed two unusual skin manifestations during an episode of visceral leishmaniasis. A 48-year-old female patient consulted initially for infiltrated purpura of all four limbs. Skin biopsy revealed leukocytoclastic vasculitis with Leishman-Donovan bodies. Laboratory tests showed medullary, splenic, gastric and colic involvement, suggesting systemic disease, and enabling visceral leishmaniasis to be diagnosed. Two years later, despite prolonged treatment, the patient presented maculopapular exanthema, and histology revealed persistent Leishman-Donovan bodies. We report herein an association of two rare skin manifestations in an HIV-positive patient with visceral leishmaniasis: infiltrated purpura and maculopapular exanthema. However, the underlying mechanisms remain hypothetical. The initial leukocytoclastic exanthema could be secondary to either polyclonal hypergammaglobulinaemia or to IgA deposits, or possibly to mechanical impairment of blood vessels by the actual parasite. The maculopapular exanthema occurring later raised the possibility of post-Kala-Azar leishmaniasis due to blood-borne dissemination in an anergic subject or perhaps even immune reconstitution inflammatory syndrome. Copyright © 2016 Elsevier Masson SAS. All rights reserved.

  19. Development and application of 'simple' diagnostic tools for visceral leishmaniasis

    NARCIS (Netherlands)

    Schallig, H. D.; Schoone, G. J.; Kroon, C. C.; Hailu, A.; Chappuis, F.; Veeken, H.

    2001-01-01

    The diagnosis of visceral leishmaniasis is difficult. Due to the limitations of direct methods to detect parasites, indirect immunological methods are widely employed. The simple affordable and sensitive/specific direct agglutination test (DAT) is perhaps the most important diagnostic tool under

  20. Magnetic resonance imaging of atherosclerosis : studies in visceral obesity

    NARCIS (Netherlands)

    Alizadeh Dehnavi, Reza

    2009-01-01

    The aim of this thesis was to explore the relation between visceral obesity and the accompanying metabolic disturbances, systemic inflammation and the atherosclerotic process. A newly developed magnetic resonance vessel wall imaging technique was implemented in phenotyping patients and as a

  1. Quantification of visceral adipose tissue in polycystic ovary syndrome

    DEFF Research Database (Denmark)

    Frøssing, Signe; Nylander, Malin Chatarina; Chabanova, Elizaveta

    2018-01-01

    Background Polycystic ovary syndrome (PCOS) is associated with frequent overweight and abdominal obesity. Quantifying visceral adipose tissue (VAT) in PCOS patients can be a tool to assess metabolic risk and monitor effects of treatment. The latest dual-energy X-ray absorptiometry (DXA) technology...

  2. Imaging the Visceral Soma: A Corporeal Feminist Interpretation

    African Journals Online (AJOL)

    denise

    The Indo-Pacific Journal of Phenomenology (IPJP) can be found at www.ipjp.org. Imaging the Visceral Soma: A Corporeal Feminist Interpretation by Ingrid Richardson and Carly Harper. Abstract. Feminist philosophers of technoscience have long argued that it is vital that we question biomedical and scientific claims to an ...

  3. Association between visceral fat, IL-8 and endometrial cancer.

    Science.gov (United States)

    Ciortea, Razvan; Mihu, Dan; Mihu, Carmen Mihaela

    2014-01-01

    In endometrial cancer, visceral obesity, as a risk factor, is associated with a chronic inflammatory process, confirmed by the elevation of serum inflammatory markers in obese patients. The aim of the present study was to evaluate the correlation between visceral fat, assessed by ultrasonography, and the systemic levels of interleukin (IL)-8 in patients with endometrial cancer. This study also evaluated the usefulness of abdominal ultrasonography in assessing the visceral fat correlated with systemic inflammatory status, as an alternative method to identify patients at risk of endometrial cancer. The study was a case-control analysis including two groups of patients: Group I: 44 patients diagnosed with endometrial cancer; group II: 44 patients with no gynecological pathology. The diagnosis of endometrial cancer was performed following histopathological examination that evaluated the tissue material obtained through endometrial biopsy. These patients underwent ultrasound examination by which intraperitoneal fat was determined. IL-8 levels were determined for each patient. The Student's t-test was used for the comparison of the means and the Mann-Whitney test for rank comparison of two independent samples. In patients diagnosed with endometrial cancer, the visceral fat area evaluated by ultrasound was significantly larger (pendometrial cancer group were significantly elevated (pendometrial cancer.

  4. Blood pressure rhythmicity and visceral fat in children with hypertension.

    Science.gov (United States)

    Niemirska, Anna; Litwin, Mieczysław; Feber, Janusz; Jurkiewicz, Elżbieta

    2013-10-01

    Primary hypertension is associated with disturbed activity of the sympathetic nervous system and altered blood pressure rhythmicity. We analyzed changes in cardiovascular rhythmicity and its relation with target organ damage during 12 months of antihypertensive treatment in 50 boys with hypertension (median, 15.0 years). The following parameters were obtained before and after 12 months of antihypertensive treatment: 24-hour ambulatory blood pressure, left ventricular mass, carotid intima-media thickness, and MRI for visceral and subcutaneous adipose tissue. Amplitudes and acrophases of mean arterial pressure and heart rate rhythms were obtained for 24-, 12-, and 8-hour periods. After 1 year of treatment, 68% of patients were normotensive, and left ventricular mass and carotid intima-media thickness decreased in 60% and 62% of patients, respectively. Blood pressure and heart rate rhythmicity patterns did not change. Changes in blood pressure amplitude correlated with the decrease of waist circumference (P=0.035). Moreover, the decrease of visceral fat correlated with the decrease of 24-hour mean arterial pressure and heart rate acrophases (both Pblood pressure and heart rate rhythms between patients who achieved or did not achieve normotension and regression of left ventricular mass and carotid intima-media thickness. It was concluded that abnormal cardiovascular rhythmicity persists in children with primary hypertension despite effective antihypertensive treatment, which suggests that it may be the primary abnormality. The correlation between changes in cardiovascular rhythmicity and visceral obesity may indicate that the visceral fat plays an important role in the sympathetic activity of adolescents with hypertension.

  5. visceral leishmaniasis with concomittant post kala-azar dermal ...

    African Journals Online (AJOL)

    hi-tech

    East African Medical Journal Vol. 80 No. 8 August 2003. VISCERAL LEISHMANIASIS WITH CONCOMITTANT POST KALA-AZAR DERMAL LEISHMANIASIS RESPONDS TO ORAL SITAMAQUINE: CASE. REPORT. J. Mbui, MBChB, MMed, MSc,Senior Research Officer, R. Rashid, MBChB, MMed, Principal Research Officer, ...

  6. Visceral leishmaniasis in Northern Ethiopia | Haile | East African ...

    African Journals Online (AJOL)

    Background: Visceral leishmaniasis (VL) has been well documented by the Medecins sans Frontieres (MSF) VL treatment programmeme in the Tigray region of Ethiopia, but reports are limited from other facilities in this region where this disease continues to cause substantial morbidity and mortality. Objective: To describe ...

  7. Visceral Pedagogies: Pornography, Affect, and Safety in the University Classroom

    Science.gov (United States)

    Paasonen, Susanna

    2016-01-01

    This article addresses the pedagogical choices and the visceral reverberations involved in teaching porn in the university classroom. The author discusses different aims and goals for teaching pornography, as well as the some key pedagogical considerations and options involved in this, drawing on her own experiences teaching porn in Finnish gender…

  8. SOROPREVALÊNCIA E VARIÁVEIS EPIDEMIOLÓGICAS ASSOCIADAS À LEISHMANIOSE VISCERAL CANINA EM ÁREA ENDÊMICA NO MUNICÍPIO DE SÃO LUÍS, MARANHÃO, BRASIL

    Directory of Open Access Journals (Sweden)

    David Soeiro Barbosa

    2010-10-01

    Full Text Available This work aimed to determine the seroprevalence and epidemiological variables associated to Leishmania spp. infection in dogs from five locations of Tirirical District, in São Luís, Maranhão State, Brazil. Seventy-two houses were visited and samples of one hundred dogs were taken. A questionnaire was applied in order to investigate the possible factors related to the Leishmania infection. The following variables were used: forest proximity from the residence, livestock of domestic animals or shelter of wild animals, sex, age and breed. Clinical examination was also performed looking for clinical signs compatible to the infection. The serological analysis revealed that 67 samples were reactive. The clinical signs observed were focal lymphadenophaty, alopecia, weight loss, cutaneous ulcers, skin desquamation, keratoconjuntivitis and onycogrifosis. Animals from Cruzeiro de Santa Bárbara and Cajupari, both locations near the forest, have 3.4 and 12.0 more chances of being reactive to Leishmania spp. than the ones in the other studied locations. No statistical relation was found between seropositive dogs and any other variables.

  9. Microbiological and histopathological aspects of canine pyometra Aspectos microbiológicos e histopatológicos da piometria canina

    Directory of Open Access Journals (Sweden)

    Jennifer Anne Coggan

    2008-09-01

    Full Text Available As pyometra is recognized as one of the main causes of disease and death in the bitch the purposes of this study were to evaluate microbiological and histopathological aspects of canine pyometra and to research the virulence factors of the E. coli isolates identifying possible risks to human health. The microbiological isolation from the intrauterine contents of 100 dogs with pyometra was carried out and the virulence factors in the E. coli strains were identified using PCR method. This study also consisted of the counting of microorganisms colonies forming units in samples of intrauterine content, tests of antimicrobial susceptibility of the E. coli isolates and the histological examination of the uterus. E. coli was the most prevalent microorganism isolated (76.6% and 120 strains (79.5% were positive for sfa, 86 (56.9% were positive for cnf, 87 (57.6% were positive for pap, 52 (34.4% were positive for hly, 51 (33.8% were positive for iuc and 5 (3.3% were positive for afa genes. One observed more sensitivity of E. coli to norfloxacin, polimixin B, sulphazotrin, chloranfenicol and enrofloxacin. In 42% of the samples of uterine walls where microorganisms were isolated, the sizes of the areas of the inflammatory responses corresponded to 39-56%. Virulence factors were identified in 98.0% of the strains evaluated, demonstrating a high frequency of potentially pathogenic E. coli. It must be considered that dogs are animals that are living in close proximity to man for thousands of years and have an important role in the transmission of E. coli to other animals and to man.A piometra é uma enfermidade da cadela adulta, sendo a doença reconhecida como uma das causas mais comuns de morte desta espécie animal. Os objetivos deste trabalho foram a avaliação de aspectos microbiológicos e histopatológicos da piometra canina e pesquisa de fatores de virulência de E. coli, identificando possíveis riscos para a saúde humana. Foi realizado o exame

  10. Brucelosis canina en perros de la ciudad de Buenos Aires Canine brucellosis in dogs in the city of Buenos Aires

    Directory of Open Access Journals (Sweden)

    Eduardo Boeri

    2008-08-01

    Full Text Available La brucelosis canina, causada por Brucella canis, provoca epididimitis, atrofia testicular y esterilidad en los perros, mientras que en las hembras el síntoma principal es el aborto. La transmisión al hombre puede ser por contacto con el semen, orina, y/o fetos abortados de animales infectados. El presente estudio de tipo observacional de corte transversal, se realizó en caninos de barrios y asentamientos con alto índice de necesidades básicas insatisfechas (NBI en 8 áreas de la ciudad de Buenos Aires. Se estudiaron 219 perros, 184 hembras y 35 machos, que fueron negativos a la prueba de aglutinación con antígeno tamponado (BPAT, que descartó la infección con especies lisas del género Brucella. Sedetectaron anticuerpos anti-B. canis en 16 perros (7.3%, 9 hembras y 7 machos, este último dato es relevante ya que la orina de los machos es considerada uno de los medios de diseminación de la infección. Aunque sólo se pudieron tomar hemocultivos a 175 animales, en 3 (2 hembras y un macho se aislaron B. canis. Sólo 3 de los dueños de los perros positivos accedieron al diagnóstico serológico y dos resultaron positivos. Destacamos que la prueba de inmunodifusión en gel de agar (IGDA ha demostrado ser poco sensible, detectó sólo uno de los 16 casos positivos y ninguno de los tres confirmados por aislamiento. Concluimos que en las áreas estudiadas el hallazgo de perros serológicamente positivos y el aislamiento de B. canis en 3 casos, son indicadores del riesgo en el que se encuentra la salud de la población expuesta.Canine brucellosis, caused by Brucella canis, provokes epidydimitis, testicular atrophy and sterility in male dogs, while in females the major symptom is miscarriage. Transmission to humans may be through contact with semen, urine and/or aborted fetuses of infected animals. Our study, observational and cross-sectional, focused on dogs in lower class neighborhoods and slums with a high rate of unmet basic needs (UBN in

  11. Melostelis gen. nov., espécies novas e notas complementares sobre Anthidiini (Hymenoptera, Apidae

    Directory of Open Access Journals (Sweden)

    Danúncia Urban

    2011-06-01

    Full Text Available Melostelis gen. nov., espécies novas e notas complementares sobre Anthidiini (Hymenoptera, Apidae. Melostelis gen. nov. é proposto para um novo Anthidiini cleptoparasita. São descritas e ilustradas duas espécies novas: Melostelis amazonensis sp. nov. de Manaus, Amazonas e Larocanthidium chacoense sp. nov. de Porto Murtinho, Mato Grosso do Sul. São dados a conhecer os machos de Epanthidium bolivianum Urban, 1995 e Epanthidium araranguense Urban, 2006 e, registrados pela primeira vez no Brasil, na sub-região do chaco, Ketianthidium zanolae Urban, 2000 e Epanthidium bolivianum.

  12. Dos notas sobre los retos para el desarrollo portuario en Colombia

    OpenAIRE

    Duque Escobar, Gonzalo

    2010-01-01

    Se presentan dos artículos de importancia para enfrentar el retraso portuario del país: ¿Un megapuerto en Bahía Málaga?, y El valor estratégico de Urabá para el desarrollo portuario del Pacífico. Cuando modernizamos a Cartagena y Buenaventura no calculamos que quedaríamos retrazados un siglo al adaptar estos puertos para naves medianas, tipo pánamax. Esta razón explica la entrega del Canal de Panamá por parte de los EEUU y su actual proyecto de ampliación. En estas notas se contemplan opcione...

  13. A síndrome piramidal e o feixe piramidal: breve nota histórica

    OpenAIRE

    Fernando Rezende-Cunha; Ricardo de Oliveira-Souza

    2011-01-01

    The discovery of the pyramidal syndrome and tract is briefly reviewed with emphasis on a few key historical aspects. The pursuit of the relationship between the lateralized deficits resulting from contralateral head trauma begins in the fourth century BC with the Hippocratic School and continues until the present day. Os autores fazem uma breve nota histórica da síndrome piramidal e do feixe piramidal no homem. Os achados de deficiências motoras decorrentes de traumatismo craniano começam ...

  14. Interest in paromomycin for the treatment of visceral leishmaniasis (kala-azar

    Directory of Open Access Journals (Sweden)

    Wiwanitkit V

    2012-06-01

    Full Text Available Viroj Wiwanitkit1–31Wiwanitkit House, Bang Khae, Bangkok, Thailand; 2Hainan Medical University, Haikou, Hainan, People's Republic of China; 3Joseph Ayo Babalola University, Ikeji-Arakeji, Osun State, NigeriaAbstract: Leishmaniasis is an important vector-borne disease, and it is classified as one of the most important tropical fly-borne infections. This disease can cause two types of clinical manifestations: cutaneous forms and visceral forms. Visceral leishmaniasis, which is also called kala-azar, is a very serious infection that can be fatal. The management of visceral leishmaniasis requires informed diagnostic and therapeutic approaches. Continuous research and development regarding the treatment of visceral leishmaniasis had led to many improvements. Paromomycin is a relatively new antibiotic drug that has been used for the treatment of visceral leishmaniasis for several years. This article reviews and discusses the use of paromomycin for visceral leishmaniasis therapy.Keywords: visceral, leishmaniasis, paromomycin

  15. Visceral adipose tissue area measurement at a single level: can it represent visceral adipose tissue volume?

    Science.gov (United States)

    Noumura, Yusuke; Kamishima, Tamotsu; Sutherland, Kenneth; Nishimura, Hideho

    2017-08-01

    Measurement of visceral adipose tissue (VAT) needs to be accurate and sensitive to change for risk monitoring. The purpose of this study is to determine the CT slice location where VAT area can best reflect changes in VAT volume and body weight. 60 plain abdominal CT images from 30 males [mean age (range) 51 (41-68) years, mean body weight (range) 71.1 (101.9-50.9) kg] who underwent workplace screenings twice within a 1-year interval were evaluated. Automatically calculated and manually corrected areas of the VAT of various scan levels using "freeform curve" region of interest on CT were recorded and compared with body weight changes. The strongest correlations of VAT area with VAT volume and body weight changes were shown in a slice 3 cm above the lower margin of L3 with r values of 0.853 and 0.902, respectively. VAT area measurement at a single level 3 cm above the lower margin of the L3 vertebra is feasible and can reflect changes in VAT volume and body weight. Advances in knowledge: As VAT area at a CT slice 3cm above the lower margin of L3 can best reflect interval changes in VAT volume and body weight, VAT area measurement should be selected at this location.

  16. Adipocitos, obesidad visceral, inflamación y enfermedad cardiovascular Adipocytes, visceral obesity, inflammation and cardiovascular disease

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    Fernando Manzur

    2010-09-01

    Full Text Available La obesidad es un importante problema de salud a nivel mundial. Se considera el resultado de la combinación de factores genéticos, alimentación inadecuada y falta de actividad física regular. La ingestión de una dieta de alta densidad energética, es la principal causa de obesidad visceral o central, ya que el exceso de energía se almacena en los adipocitos, que aumentan en tamaño y en número, o ambos, en especial los viscerales, produciendo un incremento en la tasa de lipólisis, que a su vez, estimula la secreción de citoquinas por leucocitos, macrófagos y adipocitos, y conduce a estado proinflamatorio, resistencia a la insulina y disfunción endotelial. Esta última, favorecida por el proceso inflamatorio, puede ser el vínculo de unión entre la obesidad y la enfermedad cardiovascular. Así, la disfunción del tejido adiposo representa el mecanismo etiopatogénico en el desarrollo de enfermedad cardiovascular, iniciado por la obesidad visceral.Worldwide obesity is an important health problem that results from the combination of genetic factors, inadequate food intake and lack of regular physical activity. Intake of a high energy-dense diet is the main cause of visceral and central obesity, since energy excess is stored in adipocytes that increase in size and/or number, especially visceral adipocytes, causing an increment in lipolysis rate that in turn stimulates the cytokines secretion from leucocytes, macrophages and adipocytes, leading to a pro-inflammatory state, insulin resistance and endothelial dysfunction. This endothelial dysfunction favored by the inflammatory process can be the connecting bond between obesity and cardiovascular disease. Thus, adipose tisssue dysfunction constitutes the ethio-pathogenic mechanism in the development of cardiovascular disease, initiated by visceral obesity.

  17. Estudo de flebotomíneos (Diptera: Psychodidae em foco de leishmaniose visceral no Estado de Mato Grosso do Sul, Brasil

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    Galati Eunice A. B.

    1997-01-01

    Full Text Available INTRODUÇÃO: Nas Américas, Lutzomyia longipalpis tem sido incriminada como vetora da leishmaniose visceral em, praticamente, todas as áreas de ocorrência dessa parasitose. A notificação de casos humanos a partir de 1980 e a presença de cães com aspecto sugestivo de leishmaniose visceral no Município de Corumbá, Estado de Mato Grosso do Sul, Brasil, levaram a investigações entomológicas na área, com o objetivo de identificar a população de flebotomíneo vetora. MATERIAL E MÉTODO: A pesquisa foi realizada no peri e intradomicílio de três residências urbanas e em ecótopo natural, representado por uma gruta, situado fora do perímetro urbano. As capturas, semanais em sua maioria, foram realizadas com armadilhas automáticas luminosas, no período de 1984 a 1986. Os dados metereológicos desse período foram obtidos junto à Estação Metereológica da cidade e os de 1925 a 1982, de bibliografia. RESULTADOS: A fauna flebotomínea urbana, composta de oito espécies, mostrou-se semelhante à da gruta, porém nesta, a abundância das espécies foi maior. Na área urbana, Lu. cruzi predominou tanto no intra como no peridomicílio: no bairro central, representou 90,3% dos espécimens e nos dois bairros periféricos, os seus percentuais foram menores. Lu. forattinii, também, teve freqüência expressiva em um dos bairros periféricos (39,0 %. Na gruta, Lu. corumbaensis foi a espécie predominante. Comenta-se o impacto das condições climáticas e do inseticida aplicado na área urbana na freqüência das espécies, e da utilização da gruta como criadouro pelos flebotomíneos, com base na evolução da razão entre os sexos ao longo do período. Foram adicionadas informações sobre antropofilia e de coleta com isca canina de Lu. forattinii. CONCLUSÃO: O predomínio de Lu. cruzi na área urbana; a expressiva freqüência de Lu. forattinii na periferia da cidade, bem como a sua antropofilia e o estreito grau de parentesco destas esp

  18. Estudo de flebotomíneos (Diptera: Psychodidae em foco de leishmaniose visceral no Estado de Mato Grosso do Sul, Brasil

    Directory of Open Access Journals (Sweden)

    Eunice A. B. Galati

    1997-08-01

    Full Text Available INTRODUÇÃO: Nas Américas, Lutzomyia longipalpis tem sido incriminada como vetora da leishmaniose visceral em, praticamente, todas as áreas de ocorrência dessa parasitose. A notificação de casos humanos a partir de 1980 e a presença de cães com aspecto sugestivo de leishmaniose visceral no Município de Corumbá, Estado de Mato Grosso do Sul, Brasil, levaram a investigações entomológicas na área, com o objetivo de identificar a população de flebotomíneo vetora. MATERIAL E MÉTODO: A pesquisa foi realizada no peri e intradomicílio de três residências urbanas e em ecótopo natural, representado por uma gruta, situado fora do perímetro urbano. As capturas, semanais em sua maioria, foram realizadas com armadilhas automáticas luminosas, no período de 1984 a 1986. Os dados metereológicos desse período foram obtidos junto à Estação Metereológica da cidade e os de 1925 a 1982, de bibliografia. RESULTADOS: A fauna flebotomínea urbana, composta de oito espécies, mostrou-se semelhante à da gruta, porém nesta, a abundância das espécies foi maior. Na área urbana, Lu. cruzi predominou tanto no intra como no peridomicílio: no bairro central, representou 90,3% dos espécimens e nos dois bairros periféricos, os seus percentuais foram menores. Lu. forattinii, também, teve freqüência expressiva em um dos bairros periféricos (39,0 %. Na gruta, Lu. corumbaensis foi a espécie predominante. Comenta-se o impacto das condições climáticas e do inseticida aplicado na área urbana na freqüência das espécies, e da utilização da gruta como criadouro pelos flebotomíneos, com base na evolução da razão entre os sexos ao longo do período. Foram adicionadas informações sobre antropofilia e de coleta com isca canina de Lu. forattinii. CONCLUSÃO: O predomínio de Lu. cruzi na área urbana; a expressiva freqüência de Lu. forattinii na periferia da cidade, bem como a sua antropofilia e o estreito grau de parentesco destas esp

  19. O positivismo na República (Notas sobre a história do positivismo no Brasil

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    João Cruz Costa

    1953-09-01

    Full Text Available (Primeiro Parágrafo do Artigo Estas ligeiras notas para a história do positivismo no Brasil, constituem uma pequena e modesta contribuição ao estudo das história das idéias do nosso país.

  20. Mercados de notas estructuradas. Un análisis descriptivo y métodos de evaluación

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    Francisco Venegas-Martínez

    2007-01-01

    Full Text Available El presente trabajo analiza las notas estructuradas más comunes en el mercado. Se presenta una descripción detallada de dichos instrumentos financieros destacando sus características particulares y dificultades técnicas en el proceso de evaluación. Debido a que la mayoría de las notas estructuradas que se negocian en el mercado financiero mexicano son certificados de depósito con garantía del capital inicial, este trabajo proporciona los elementos básicos que se requieren para su evaluación, como son los bonos cuponados flotantes y los productos de las tasas de interés. Asimismo, para la mayoría de las notas estructuradas estudiadas aquí se desarrollan modelos teóricos de evaluación. Por último, con fines ilustrativos, varios ejemplos numéricos acerca de las notas son presentados.

  1. De gevolgen van beleidsmaatregelen uit de Nota Wonen op bewegingsarmoede in Nederland. Onderdeel van de gezondheidseffectrapportage "Mensen wensen gezond wonen"

    NARCIS (Netherlands)

    Wendel-Vos GCW; Schuit AJ; Seidell JC; CZE

    2003-01-01

    Health gains can be achieved by actively combating and decreasing physical inactivity. One of the factors probably influencing physical activity is the living environment. The Policy Document on Housing ('Nota Wonen' in Dutch) presents policy measures influencing the human living environment. The

  2. Sexually dimorphic effects of unpredictable early life adversity on visceral pain behavior in a rodent model.

    Science.gov (United States)

    Chaloner, Aaron; Greenwood-Van Meerveld, Beverley

    2013-03-01

    Visceral pain is the hallmark feature of irritable bowel syndrome (IBS), a gastrointestinal disorder, which is more commonly diagnosed in women. Female IBS patients frequently report a history of early life adversity (ELA); however, sex differences in ELA-induced visceral pain and the role of ovarian hormones have yet to be investigated. Therefore, we tested the hypothesis that ELA induces visceral hypersensitivity through a sexually dimorphic mechanism mediated via estradiol. As a model of ELA, neonatal rats were exposed to different pairings of an odor and shock to control for trauma predictability. In adulthood, visceral sensitivity was assessed via a visceromotor response to colorectal distension. Following ovariectomy and estradiol replacement in a separate group of rats, the visceral sensitivity was quantified. We found that females that received unpredictable odor-shock developed visceral hypersensitivity in adulthood. In contrast, visceral sensitivity was not significantly different following ELA in adult males. Ovariectomy reversed visceral hypersensitivity following unpredictable ELA, whereas estradiol replacement reestablished visceral hypersensitivity in the unpredictable group. This study is the first to show sex-related differences in visceral sensitivity following unpredictable ELA. Our data highlight the activational effect of estradiol as a pivotal mechanism in maintaining visceral hypersensitivity. This article directly implicates a critical role for ovarian hormones in maintaining visceral hypersensitivity following ELA, specifically identifying the activational effect of estradiol as a key modulator of visceral sensitivity. These data suggest that ELA induces persistent functional abdominal pain in female IBS patients through an estrogen-dependent mechanism. Copyright © 2013 American Pain Society. All rights reserved.

  3. KSAC, the first defined polyprotein vaccine candidate for visceral leishmaniasis.

    Science.gov (United States)

    Goto, Yasuyuki; Bhatia, Ajay; Raman, Vanitha S; Liang, Hong; Mohamath, Raodoh; Picone, Alessandro F; Vidal, Silvia E Z; Vedvick, Thomas S; Howard, Randall F; Reed, Steven G

    2011-07-01

    A subunit vaccine using a defined antigen(s) may be one effective solution for controlling leishmaniasis. Because of genetic diversity in target populations, including both dogs and humans, a multiple-antigen vaccine will likely be essential. However, the cost of a vaccine to be used in developing countries must be considered. We describe herein a multiantigen vaccine candidate comprised of antigens known to be protective in animal models, including dogs, and to be recognized by humans immune to visceral leishmaniasis. The polyprotein (KSAC) formulated with monophosphoryl lipid A, a widely used adjuvant in human vaccines, was found to be immunogenic and capable of inducing protection against Leishmania infantum, responsible for human and canine visceral leishmaniasis, and against L. major, responsible for cutaneous leishmaniasis. The results demonstrate the feasibility of producing a practical, cost-effective leishmaniasis vaccine capable of protecting both humans and dogs against multiple Leishmania species.

  4. Visceral Leishmaniasis in Latin America and therapy perspectives

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    Catalina Tovar A

    2017-05-01

    Full Text Available In Latin America, visceral leishmaniasis is caused by Leishmania infantum. In this geographical area, main vectors associated with transmission are Lutzomyia longipalpis and Lutzomyia evansi, with dogs being incriminated as the main reservoir involved in transmission of the disease. This pathology primarily affects children between 0 - 5 years, a highly susceptible population where socio-economic, environmental and nutritional factors affects the pathological outcome and increase the likelihood of vector-human contact. According to the World Health Organization (WHO recommended treatment for Visceral Leishmaniasis is liposomal amphotericin B, a drug with a limited and variable availability between countries depending on market prices, which leaves pentavalent antimonial as the most widely used treatment despite the associated toxic effects. In the Americas, evidence on the efficacy of single-dose (monotherapy and combination therapies as options for treating these parasites is required.

  5. Host resistance to visceral leishmaniasis: prevalence and prevention.

    Science.gov (United States)

    Maran, Naiara; Gomes, Pollyanna Stephanie; Freire-de-Lima, Leonardo; Freitas, Elisangela Oliveira; Freire-de-Lima, Célio Geraldo; Morrot, Alexandre

    2016-01-01

    Visceral leishmaniasis (VL) is a chronic parasitic disease caused by the vector-borne Leishmania donovani and Leishmania (L.) infantum chagasi parasites. The disease affects about 12 million humans in more than 90 countries worldwide. If not treated, the visceral form of Leishmania infection is potentially fatal, yielding about 50000 deaths per year. In the vertebrate host, the Leishmania species causing VL spread systematically to propagate in macrophage reservoirs distributed in the tissues of internal organs, primarily the liver, spleen, bone marrow and the lymph nodes. The infection is associated with evolved mechanisms from the parasite to subvert the host immune system in order to establish a persistent infection. Currently, efforts are being deployed to develop new anti-leishmanial therapies in VL combining immunomodulatory treatment regimens that burst the host immune responses together with leishmanicidal drugs that target the parasite growth. Discoveries in this field are discussed in this article.

  6. Scintigraphy usefulness in the diagnosis of visceral candidiasis

    Energy Technology Data Exchange (ETDEWEB)

    Ythier, H.; Legghe, R.; Foucher, C.

    1987-01-01

    From the features of two cases, the authors stress the usefulness of the scintigraphy as regards to the diagnosis of visceral candidial abscesses. Such fungal localisations are not unfrequent, especially in immunodeficient patients (haematologic malignancies undergoing chemotherapy, lupus, serious visceral illness...). The positive diagnosis is uneasy because of non-specific clinical features and frequent negative blood cultures. Splenic localisation is the most likely. Citrate Gallium scintigraphy together with splenic labelled RBC scan enables us to give a precise view of the splenic involvment and even of the abdominal extension of the fungal abscess. From the literature review and these two cases, the excellent adequacy of the scintigraphy to the follow-up of systemic candidiasis is underlined and is compared to other usual morphological studies such as US scan and CT examination. In both cases, the diagnosis is fully confirmed by mycological examination.

  7. Leishmaniose visceral: estudo retrospectivo de fatores associados à letalidade Visceral leishmaniasis: retrospective study on factors associated with lethality

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    Daniel Gomes de Alvarenga

    2010-04-01

    Full Text Available INTRODUÇÃO: A leishmaniose visceral é um problema de saúde pública, com grau de letalidade alcançando 10%. Para o tratamento medicamentoso, é recomendado o antimoniato de metilglucamina. Este estudo tem como objetivo avaliar o uso de medicamento em casos de leishmaniose visceral atendidos no Serviço de Infectologia do Núcleo de Hospital Universitário de Campo Grande Estado do Mato Grosso do Sul. MÉTODOS: Para coleta de dados, foram pesquisados prontuários de 76 pacientes com diagnóstico de leishmaniose visceral atendidos pelo Serviço de Infectologia do Hospital Universitário de Campo Grande. RESULTADOS: Foram analisados prontuários de 76 (28,9% pacientes (56 homens e 20 mulheres apresentavam comorbidades. Como droga de 1ª escolha, 88,2% dos pacientes utilizaram o antimoniato-N-metil glucamina com evolução para óbito de 18,4%. A análise de sobrevida mostrou diferença estatisticamente significativa em pacientes com e sem comorbidades (pINTRODUCTION: Visceral leishmaniasis is a public health problem, with lethality reaching 10%. The recommended drug treatment is methylglucamine antimoniate. This study aimed to evaluate drug use for cases of visceral leishmaniasis treated at the Infectology Clinic of the Campo Grande University Hospital Center, State of Mato Grosso do Sul. METHODS: To collect data, we examined the medical records of 76 patients with a diagnosis of visceral leishmaniasis treated at this Infectology Clinic. RESULTS: The medical files of 76 patients (56 men and 20 women; 28.9% showed comorbidities. The first choice drug for 88.2% of the patients was N-methylglucamine antimoniate, with a fatal outcome for 18.4%. Survival analysis showed a statistically significant difference between patients with and without comorbidities (p <0.0001 and with comorbidities who used Glucantime® (p < 0.0009. The fatality rate of 18.4% indicates the low efficiency of the healthcare measures used. CONCLUSIONS: The results suggest that

  8. Comparative Evaluation of Anti-HER2 Affibody Molecules Labeled with 64Cu Using NOTA and NODAGA

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    Vladimir Tolmachev

    2017-01-01

    Full Text Available Imaging using affibody molecules enables discrimination between breast cancer metastases with high and low expression of HER2, making appropriate therapy selection possible. This study aimed to evaluate if the longer half-life of 64Cu (T1/2 = 12.7 h would make 64Cu a superior nuclide compared to 68Ga for PET imaging of HER2 expression using affibody molecules. The synthetic ZHER2:S1 affibody molecule was conjugated with the chelators NOTA or NODAGA and labeled with 64Cu. The tumor-targeting properties of 64Cu-NOTA-ZHER2:S1 and 64Cu-NODAGA-ZHER2:S1 were evaluated and compared with the targeting properties of 68Ga-NODAGA-ZHER2:S1 in mice. Both 64Cu-NOTA-ZHER2:S1 and 64Cu-NODAGA-ZHER2:S1 demonstrated specific targeting of HER2-expressing xenografts. At 2 h after injection of 64Cu-NOTA-ZHER2:S1, 64Cu-NODAGA-ZHER2:S1, and 68Ga-NODAGA-ZHER2:S1, tumor uptakes did not differ significantly. Renal uptake of 64Cu-labeled conjugates was dramatically reduced at 6 and 24 h after injection. Notably, radioactivity uptake concomitantly increased in blood, lung, liver, spleen, and intestines, which resulted in decreased tumor-to-organ ratios compared to 2 h postinjection. Organ uptake was lower for 64Cu-NODAGA-ZHER2:S1. The most probable explanation for this biodistribution pattern was the release and redistribution of renal radiometabolites. In conclusion, monoamide derivatives of NOTA and NODAGA may be suboptimal chelators for radiocopper labeling of anti-HER2 affibody molecules and, possibly, other scaffold proteins with high renal uptake.

  9. Canine Visceral Leishmaniasis, United States and Canada, 2000–2003

    OpenAIRE

    Duprey, Zandra H.; Steurer, Francis J.; Rooney, Jane A.; Kirchhoff, Louis V.; Jackson, Joan E.; Rowton, Edgar D.; Peter M. Schantz

    2006-01-01

    Visceral leishmaniasis, caused by protozoa of the genus Leishmania donovani complex, is a vectorborne zoonotic infection that infects humans, dogs, and other mammals. In 2000, this infection was implicated as causing high rates of illness and death among foxhounds in a kennel in New York. A serosurvey of >12,000 foxhounds and other canids and 185 persons in 35 states and 4 Canadian provinces was performed to determine geographic extent, prevalence, host range, and modes of transmission within...

  10. Gut Chemosensing: Interactions between Gut Endocrine Cells and Visceral Afferents

    OpenAIRE

    Raybould, Helen E.

    2009-01-01

    Chemosensing in the gastrointestinal tract is less well understood than many aspects of gut mechanosensitivity; however, it is important in the overall function of the GI tract and indeed the organism as a whole. Chemosensing in the gut represents a complex interplay between the function of enteroendocrine (EEC) cells and visceral (primarily vagal) afferent neurons. In this brief review, I will concentrate on new data on endocrine cells in chemosensing in the GI tract, in particular on new fi...

  11. Reduced cortical thickness associated with visceral fat and BMI

    OpenAIRE

    Veit, Ralf; Kullmann, Stephanie; Heni, Martin; Machann, Jürgen; Häring, Hans-Ulrich; Fritsche, Andreas; Preissl, Hubert

    2014-01-01

    Structural brain imaging studies have shown that obesity is associated with widespread reductions in gray matter (GM) volume. Although the body mass index (BMI) is an easily accessible anthropometric measure, substantial health problems are more related to specific body fat compartments, like visceral adipose tissue (VAT). We investigated cortical thickness measures in a group of 72 healthy subjects (BMI range 20–35 kg/m2, age range 19–50 years). Multiple regression analyses were performed us...

  12. Visceral leishmaniasis masquerading as tuberculosis in a patient with AIDS

    Science.gov (United States)

    Yaduvanshi, A.; Jain, M.; Jain, S; Jain, S.; Arora, S.

    1999-01-01

    We report a case of visceral leishmaniasis presenting as significant lymphadenopathy in a patient with acquired immune deficiency syndrome. The lymphadenopathy was initially suspected to be tubercular in nature on pathological examination. This report highlights the increasing incidence of acquired immune deficiency syndrome and Leishmania co-infection in India, and the importance of demonstrating tubercle bacilli on culture before suggesting a diagnosis of tuberculosis.


Keywords: leishmaniasis; AIDS; tuberculosis PMID:10567601

  13. Infecções experimentaes na Leishmaniose visceral americana Experimental infections in american visceral leishmaniasis

    Directory of Open Access Journals (Sweden)

    Aristides Marques da Cunha

    1938-01-01

    Full Text Available Passando em revista as experiencias de infecção que effectuamos e que acabamos de relatar, vemos que, as culturas isoladas de casos de Leishmaniose visceral americana quer do homem quer do cão, são capazes de infectar hamsters, rhesus e cães de maneira identica ao que acontece com as outras formas de Kala-Azar. Notamos ainda que a evolução da doença e as lesões observadas nos animaes assim infectados, se assemelham ao que tem sido observado nessa enfermidade aproximando-a de maneira singular do Kala-Azar mediterraneo. É sobretudo no cão que a semelhança se torna mais patente. A infecção da pelle e as lesões por ella provocadas que observamos, não differem em nada das que tem sido descriptas na infecção natural do cão e já assignaladas tambem na infecção experimental desse animal, embora de maneira menos completa do que fazemos agora aqui. As lesões oculares com formação de placas de keratite, a infeccção massiça do intestino nos casos fataes de infecção e até mesmo as lesões do figado descriptas por Adler como peculiares á infecção experimental, (infiltração em torno da veia central do lobulo constituem outros tantos caracteres que mostram a completa analogia entre as infecções do cão que obtivemos e as já observadas no Kala-Azar mediterraneo. Além disso, a presença de Leishmanias na pelle, as vezes em grande numero e a constancia dessa localisação parasitaria, vem mostrar que o cão apresenta as condições necessarias para desempenhar o papel de depositario de virus tal como acontece no Kala-Azar mediterraneo, tanto mais que é elle encontrado naturalmente infectado, nas regiões em que grassa a doença. Mas não é só a infecção do cão que mostra essa semelhança; nos outros animaes tambem se verifica o mesmo facto e até pequenos signaes, taes como a tumefacção das patas assignalada nos hamsters infectados com Leishmania infantum, foram tambem observados aqui. Por outro lado, a reacção de

  14. Role of Nampt and Visceral Adiposity in Esophagogastric Junction Adenocarcinoma

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    Haijun Li

    2017-01-01

    Full Text Available Nampt including eNampt and iNampt may contribute to mediating obesity-associated cancers. This study investigated the role of Nampt in esophagogastric junction adenocarcinoma (EGA, a cancer strongly correlated with obesity. Visceral adiposity was defined by waist circumference or VFA. eNampt in sera were measured by enzyme-linked immunosorbent assay. iNampt expression in EGA was determined by PCR, western blot, and immunohistochemistry. Sera eNampt were significantly elevated in these overweight and obese patients, especially for viscerally obese patients, and positively correlated with BMI, waist circumference, VFA, and also primary tumor, regional lymph nodes, and TNM stage (P<0.05. iNampt expression in both the mRNA and protein levels was upregulated in EGAs (P<0.05. iNampt staining was found primarily in the cytoplasm and nuclei and significantly associated with tumor, lymph nodes, and TNM stage and also correlated positively with serum eNampt, BMI, total fat area, VFA, superficial fat area, and waist circumference (P<0.05. iNampt, eNampt, tumor, lymph nodes, and TNM stage correlated to the survival of EGAs, and iNampt expression and TNM stage affected the prognosis independently (P<0.05. This study highlighted the association of eNampt/iNampt with visceral obesity and a potential impact on the biology of EGA.

  15. Negative mood affects brain processing of visceral sensation.

    Science.gov (United States)

    Coen, Steven J; Yágüez, Lidia; Aziz, Qasim; Mitterschiffthaler, Martina T; Brammer, Mick; Williams, Steven C R; Gregory, Lloyd J

    2009-07-01

    A link between negative emotional state and abnormal visceral sensation has been frequently reported. However, the influence of negative emotion on brain processing of painful visceral sensations has not been investigated. We used functional magnetic resonance imaging (fMRI) and negative emotional stimuli to investigate the effects of negative emotion on brain processing of esophageal sensation. Twelve healthy male volunteers (age range, 21-32 years) participated in the study. Negative emotion was induced using emotionally valent music. fMRI images were acquired during 2 experimental runs; throughout these, volunteers received randomized nonpainful and painful distentions to the esophagus during neutral and negative emotion. Subjective perception of each stimulus was acquired, as were mood ratings. Sadness ratings increased significantly following negative mood induction (P .05). Following painful stimulation, brain activity increased in the right hemisphere during negative emotion and was localized to the anterior cingulate cortex (ACC; BA24/32), anterior insula, and inferior frontal gyrus. Following nonpainful stimulation during negative emotion, brain activity increased in the right anterior insula and ACC (BA24 and 32). This study provides new information about the influence of negative affect on central processing of visceral pain. Evidence of right hemispheric dominance during negative emotion indicates this hemisphere is predominately associated with sympathetic activity (arousal, negative affect) and that the right insula and right ACC are integral to subjective awareness of emotion through interoception.

  16. Effects of visceral adiposity on glycerol pathways in gluconeogenesis.

    Science.gov (United States)

    Neeland, Ian J; Hughes, Connor; Ayers, Colby R; Malloy, Craig R; Jin, Eunsook S

    2017-02-01

    To determine the feasibility of using oral 13 C labeled glycerol to assess effects of visceral adiposity on gluconeogenic pathways in obese humans. Obese (BMI ≥30kg/m 2 ) participants without type 2 diabetes underwent visceral adipose tissue (VAT) assessment and stratification by median VAT into high VAT-fasting (n=3), low VAT-fasting (n=4), and high VAT-refed (n=2) groups. Participants ingested [U- 13 C 3 ] glycerol and blood samples were subsequently analyzed at multiple time points over 3h by NMR spectroscopy. The fractions of plasma glucose (enrichment) derived from [U- 13 C 3 ] glycerol via hepatic gluconeogenesis, pentose phosphate pathway (PPP), and tricarboxylic acid (TCA) cycle were assessed using 13 C NMR analysis of glucose. Mixed linear models were used to compare 13 C enrichment in glucose between groups. Mean age, BMI, and baseline glucose were 49years, 40.1kg/m 2 , and 98mg/dl, respectively. Up to 20% of glycerol was metabolized in the TCA cycle prior to gluconeogenesis and PPP activity was minor (gluconeogenesis from glycerol in obese humans. Our findings provide preliminary evidence that excess visceral fat disrupts multiple pathways in hepatic gluconeogenesis from glycerol. Copyright © 2016 Elsevier Inc. All rights reserved.

  17. Early visceral pain predicts chronic pain after laparoscopic cholecystectomy.

    Science.gov (United States)

    Blichfeldt-Eckhardt, Morten Rune; Ording, Helle; Andersen, Claus; Licht, Peter B; Toft, Palle

    2014-11-01

    Chronic pain after laparoscopic cholecystectomy is related to postoperative pain during the first postoperative week, but it is unknown which components of the early pain response is important. In this prospective study, 100 consecutive patients were examined preoperatively, 1 week postoperatively, and 3, 6, and 12 months postoperatively for pain, psychological factors, and signs of hypersensitivity. Overall pain, incisional pain (somatic pain component), deep abdominal pain (visceral pain component), and shoulder pain (referred pain component) were registered on a 100-mm visual analogue scale during the first postoperative week. Nine patients developed chronic unexplained pain 12 months postoperatively. In a multivariate analysis model, cumulated visceral pain during the first week and number of preoperative biliary pain attacks were identified as independent risk factors for unexplained chronic pain 12 months postoperatively. There were no consistent signs of hypersensitivity in the referred pain area either pre- or postoperatively. There were no significant associations to any other variables examined. The risk of chronic pain after laparoscopic cholecystectomy is relatively low, but significantly related to the visceral pain response during the first postoperative week. Copyright © 2014 International Association for the Study of Pain. Published by Elsevier B.V. All rights reserved.

  18. Utilização de ferramentas de análise espacial na vigilância epidemiológica de leishmaniose visceral americana - Araçatuba, São Paulo, Brasil, 1998-1999

    Directory of Open Access Journals (Sweden)

    Camargo-Neves Vera Lucia Fonseca de

    2001-01-01

    Full Text Available O controle da Leishmaniose Visceral Americana (LVA está fundamentado no combate ao vetor e na eliminação do reservatório doméstico da área de foco - 200 metros em torno do caso humano ou canino. O presente trabalho tem como objetivo discutir a utilização de técnicas de análise espacial na vigilância epidemiológica da LVA no Município de Araçatuba, São Paulo, buscando estabelecer um modelo de vigilância epidemiológica em base territorial, redirecionando as estratégias de controle atualmente adotadas. Verificou-se que a transmissão da LVA não foi homogênea no município: a transmissão humana ocorreu nas áreas com maiores taxas de prevalência canina. A dispersão do vetor parece restrita a poucos domicílios, embora não tenha sido possível estabelecer um perfil da densidade vetorial. Visando estudar a distribuição do vetor e variáveis correlacionadas está sendo realizado estudo de campo por amostragem de domicílios, que permitirá o desenvolvimento de novas ferramentas de análise espacial e, possivelmente, permitirá redefinir as propostas de controle da endemia em ambiente urbano.

  19. Avaliação da infectividade parasitária a Lutzomyia longipalpis por xenodiagnóstico em cães tratados para leishmaniose visceral naturalmente adquirida

    Directory of Open Access Journals (Sweden)

    Gabriela Nery

    Full Text Available RESUMO: O efeito de um protocolo quimioterápico multidrogas contra a leishmaniose visceral (LV canina, sobre a capacidade de transmissão de Leishmania infantum ao vetor, foi analisado por meio de xenodiagnóstico. Trinta e cinco cães naturalmente infectados foram avaliados antes e durante o tratamento com a combinação de metronidazol, cetoconazol e alopurinol a cada três meses por até um ano. Em cada avaliação, os cães foram individualmente submetidos ao xenodiagnóstico e quantificação da carga parasitária por PCR quantitativa. O tratamento foi eficaz em bloquear a transmissibilidade parasitária do cão para o flebotomíneo (p= 0,011 nos cães avaliados. Houve significante correlação entre recuperação clínica e infectividade: cães com melhora clínica mais evidente apresentaram menores chances de transferir L. infantum ao Lutzomyia longipalpis via xenodiagnóstico (r=0,528, p= 0,002. Esses resultados demonstram que o tratamento canino com o protocolo proposto pode representar uma alternativa ao sacrifício de cães no Brasil como medida de controle da doença, uma vez que as drogas utilizadas não são aplicadas ao tratamento da LV humana em áreas endêmicas.

  20. Aspectos epidemiológicos sobre mordeduras caninas durante el año 2011 en la ciudad de Temuco, Chile.

    Directory of Open Access Journals (Sweden)

    Waldo Armstrong,

    2016-03-01

    Full Text Available Los accidentes causados por mordeduras de perros y gatos son un problema de salud pública y concretamente las mordeduras de perro constituyen el riesgo más grave que estos animales suponen para la salud de las personas. A los costes económicos derivados del tratamiento de las lesiones físicas debemos añadir las secuelas psicológicas que muchas veces aparecen tras las agresiones. La prevención es el único camino para resolver este problema y puede efectuarse mejor cuando se conocen los diversos factores involucrados en la génesis de este tipo de accidentedebido a lo anterior el objetivo del estudio fue describir las características epidemiológicas asociadas a las personas mordidas por perros. La metodología utilizada para la recolección de datos fue la aplicación de una encuesta a 9 centros asistenciales de la ciudad de Temuco. Se solicitó información sobre pacientes atendidos por mordeduras caninas durante el año 2011 y los datos recolectados se ingresaron en una planilla de cálculos. Los resultados indican que niños y adultos presentan 52% de las mordeduras durante el año. Los hombres presentan una mayor incidencia con un 47% a diferencia de las mujeres con un 44%. Con respecto a la región corporal afectada los miembros superiores alcanzan un 25% de frecuencia ante el ataque y los miembros inferiores un 31.3%. Por otra parte, las mordeduras por perros presentaron una marcada estacionalidad en los meses de verano con un 30% de casos. Se concluye que las mordeduras caninas son un problema frecuente y de real importancia en el ámbito de salud pública, generando en la población secuelas físicas y psicológicas por las lesiones generadas durante el ataque.

  1. Genome-wide association for abdominal subcutaneous and visceral adipose reveals a novel locus for visceral fat in women

    DEFF Research Database (Denmark)

    Fox, Caroline S; Liu, Yongmei; White, Charles C

    2012-01-01

    of European ancestry. Subcutaneous and visceral fat were quantified in 5,560 women and 4,997 men from 4 population-based studies. Genome-wide genotyping was performed using standard arrays and imputed to ~2.5 million Hapmap SNPs. Each study performed a genome-wide association analysis of subcutaneous adipose...... tissue (SAT), visceral adipose tissue (VAT), VAT adjusted for body mass index, and VAT/SAT ratio (a metric of the propensity to store fat viscerally as compared to subcutaneously) in the overall sample and in women and men separately. A weighted z-score meta-analysis was conducted. For the VAT/SAT ratio......-specific analyses. Our most significant finding was for VAT in women, rs1659258 near THNSL2 (p = 1.6 × 10-08), but not men (p = 0.75). Validation of this SNP in the GIANT consortium data demonstrated a similar sex-specific pattern, with observed significance in women (p = 0.006) but not men (p = 0.24) for BMI...

  2. Systemic arteriosclerosis and eating behavior in Japanese type 2 diabetic patients with visceral fat accumulation.

    Science.gov (United States)

    Fukuda, Shiro; Hirata, Ayumu; Nishizawa, Hitoshi; Nagao, Hirofumi; Kashine, Susumu; Kimura, Takekazu; Inoue, Kana; Fujishima, Yuya; Yamaoka, Masaya; Kozawa, Junji; Kitamura, Tetsuhiro; Yasuda, Tetsuyuki; Maeda, Norikazu; Imagawa, Akihisa; Funahashi, Tohru; Shimomura, Iichiro

    2015-01-16

    Visceral fat accumulation is a major etiological factor in the progression of type 2 diabetes mellitus and atherosclerosis. We described previously visceral fat accumulation and multiple cardiovascular risk factors in a considerable number of Japanese non-obese subjects (BMI arteriosclerosis, serum adiponectin concentration, and eating behavior in type 2 diabetic patients with and without visceral fat accumulation. The study subjects were 75 Japanese type 2 diabetes mellitus (age: 64.8 ± 11.5 years, mean ± SD). Visceral fat accumulation represented an estimated visceral fat area of 100 cm(2) using the bioelectrical impedance analysis method. Subjects were divided into two groups; with (n = 53) and without (n = 22) visceral fat accumulation. Systemic arteriosclerosis was scored for four arteries by ultrasonography. Eating behavior was assessed based on The Guideline for Obesity questionnaire issued by the Japan Society for the Study of Obesity. The visceral fat accumulation (+) group showed significantly higher systemic vascular scores and significantly lower serum adiponectin levels than the visceral fat accumulation (-) group. With respect to the eating behavior questionnaire items, (+) patients showed higher values for the total score and many of the major sub-scores than (-) patients. Type 2 diabetic patients with visceral fat accumulation showed 1) progression of systemic arteriosclerosis, 2) low serum adiponectin levels, and 3) differences in eating behavior, compared to those without visceral fat accumulation. Taken together, the findings highlight the importance of evaluating visceral fat area in type 2 diabetic patients. Furthermore, those with visceral fat accumulation might need to undergo more intensive screening for systemic arteriosclerosis and consider modifying their eating behaviors.

  3. (67/68)Ga-labeling agent that liberates (67/68)Ga-NOTA-methionine by lysosomal proteolysis of parental low molecular weight polypeptides to reduce renal radioactivity levels.

    Science.gov (United States)

    Uehara, Tomoya; Rokugawa, Takemi; Kinoshita, Mai; Nemoto, Souki; Fransisco Lazaro, Guerra Gomez; Hanaoka, Hirofumi; Arano, Yasushi

    2014-11-19

    The renal localization of gallium-67 or gallium-68 ((67/68)Ga)-labeled low molecular weight (LMW) probes such as peptides and antibody fragments constitutes a problem in targeted imaging. Wu et al. previously showed that (67)Ga-labeled S-2-(4-isothiocyanatobenzyl)-1,4,7-triazacyclononane-1,4,7-triacetic acid (SCN-Bz-NOTA)-conjugated methionine ((67)Ga-NOTA-Met) was rapidly excreted from the kidney in urine following lysosomal proteolysis of the parental (67)Ga-NOTA-Bz-SCN-disulfide-stabilized Fv fragment (Bioconjugate Chem., (1997) 8, 365-369). In the present study, a new (67/68)Ga-labeling reagent for LMW probes that liberates (67/68)Ga-NOTA-Met was designed, synthesized, and evaluated using longer-lived (67)Ga in order to reduce renal radioactivity levels. We employed a methionine-isoleucine (MI) dipeptide bond as the cleavable linkage. The amine residue of MI was coupled with SCN-Bz-NOTA for (67)Ga-labeling, while the carboxylic acid residue of MI was derivatized to maleimide for antibody conjugation in order to synthesize NOTA-MI-Mal. A Fab fragment of the anti-Her2 antibody was thiolated with iminothiolane, and NOTA-MI-Mal was conjugated with the antibody fragment by maleimide-thiol chemistry. The Fab fragment was also conjugated with SCN-Bz-NOTA (NOTA-Fab) for comparison. (67)Ga-NOTA-MI-Fab was obtained at radiochemical yields of over 95% and was stable in murine serum for 24 h. In the biodistribution study using normal mice, (67)Ga-NOTA-MI-Fab registered significantly lower renal radioactivity levels from 1 to 6 h postinjection than those of (67)Ga-NOTA-Fab. An analysis of urine samples obtained 6 h after the injection of (67)Ga-NOTA-MI-Fab showed that the majority of radioactivity was excreted as (67)Ga-NOTA-Met. In the biodistribution study using tumor-bearing mice, the tumor to kidney ratios of (67)Ga-NOTA-MI-Fab were 4 times higher (6 h postinjection) than those of (67)Ga-NOTA-Fab. Although further studies including the structure of radiometabolites and

  4. The Relation between Visceral and Subcutaneous Fat to Bone Mass among Egyptian Children and Adolescents

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    Sahar A. El-Masry

    2014-12-01

    CONCLUSIONS: Visceral and subcutaneous fat had significant positive association with bone mass in children; males and females respectively. On the contrary such association disappeared during adolescence.

  5. Visceral and subcutaneous fat have different origins and evidence supports a mesothelial source.

    Science.gov (United States)

    Chau, You-Ying; Bandiera, Roberto; Serrels, Alan; Martínez-Estrada, Ofelia M; Qing, Wei; Lee, Martin; Slight, Joan; Thornburn, Anna; Berry, Rachel; McHaffie, Sophie; Stimson, Roland H; Walker, Brian R; Chapuli, Ramon Muñoz; Schedl, Andreas; Hastie, Nick

    2014-04-01

    Fuelled by the obesity epidemic, there is considerable interest in the developmental origins of white adipose tissue (WAT) and the stem and progenitor cells from which it arises. Whereas increased visceral fat mass is associated with metabolic dysfunction, increased subcutaneous WAT is protective. There are six visceral fat depots: perirenal, gonadal, epicardial, retroperitoneal, omental and mesenteric, and it is a subject of much debate whether these have a common developmental origin and whether this differs from that for subcutaneous WAT. Here we show that all six visceral WAT depots receive a significant contribution from cells expressing Wt1 late in gestation. Conversely, no subcutaneous WAT or brown adipose tissue arises from Wt1-expressing cells. Postnatally, a subset of visceral WAT continues to arise from Wt1-expressing cells, consistent with the finding that Wt1 marks a proportion of cell populations enriched in WAT progenitors. We show that all visceral fat depots have a mesothelial layer like the visceral organs with which they are associated, and provide several lines of evidence that Wt1-expressing mesothelium can produce adipocytes. These results reveal a major ontogenetic difference between visceral and subcutaneous WAT, and pinpoint the lateral plate mesoderm as a major source of visceral WAT. They also support the notion that visceral WAT progenitors are heterogeneous, and suggest that mesothelium is a source of adipocytes.

  6. Altered Insula Activity during Visceral Interoception in Weight-Restored Patients with Anorexia Nervosa

    National Research Council Canada - National Science Library

    Kerr, Kara L; Moseman, Scott E; Avery, Jason A; Bodurka, Jerzy; Zucker, Nancy L; Simmons, W Kyle

    2016-01-01

    .... Aberrant visceral interoceptive processing within the insula has been hypothesized to be an important mechanism in AN's pathophysiology due to the theoretical link between interoception and emotional experience...

  7. Biogeochemical characteristics of Rosa canina grown in hydrothermally contaminated soils of the Gümüşhane Province, Northeast Turkey.

    Science.gov (United States)

    Vural, Alaaddin

    2015-08-01

    Kırkpavli alteration area (Gümüşhane, Northeast Turkey) is contaminated by heavy metals such as Cd, Pb, As, Cu and Zn. The quantity of accumulation of heavy metal trace elements and macroelements in 32 leaves of Rosa canina of the Kırkpavli alteration area has been studied within the scope of geochemical studies. Element contents of samples were assessed using various parameters including descriptive statistics, factor analysis, correlation coefficients and bioaccumulation factor. Concentrations were detected in the acceptable range for Mo, Cu, Pb, Ni, As, Cd, Sb, P, Ti, Na, Se and Sn. Concentrations of Co, Mn, Ba and Hg were detected close to the acceptable values, whereas Zn, Fe, Sr, V, Ca, Cr, Mg, B, Al, K, W, Sc, Cs and Rb concentrations were detected above the acceptable values. Principal component analysis was used to identify the elements that have a close relationship with each other and/or similar origins. It has been concluded that Zn, Cu, As and Mo content of the plant were related to hydrothermal alteration process and they behaved together, whereas Mn and Fe were especially products of weathering conditions, also behaved together. In terms of macroelements, Ca, Mg and Na had similar behaviour, while P and K had the same correlation.

  8. Importancia y propiedades físico química de la Rosa mosqueta (R. canina, R. rubiginosa: una revisión

    Directory of Open Access Journals (Sweden)

    T. Espinoza

    2016-01-01

    Full Text Available Los consumidores cada día exigen productos en sus dietas que no solo aporten los nutrientes requeridos para una vida sana, sino que además son de preferencia aquellos que puedan complementar con propiedades benéficas para la salud. En las últimas décadas se ha reconocido a la Rosa mosqueta, la Rosa canina y a la Rosa rubiginosa, como frutos que contienen muchas propiedades nutracéuticas. El presente trabajo hace una revisión de los artículos de investigación realizados a la Rosa mosqueta desde un punto de vista de su importancia de su consumo y sus beneficios para la salud; describiendo las características químicas, físicas y bioquímicas tales como el contenido de componentes fenólicos, ácidos grasos, ácido linoleico, minerales; y de otros compuestos importantes también desde el punto de vista industrial, tales como el contenido de antioxidantes, pigmentos, entre otros. De acuerdo a los antecedentes recopilados, la Rosa mosqueta es una fuente de micronutrientes incluyendo la vitamina c y el licopeno, entregando además un buen aporte nutricional; por lo anterior debe ser considerado como un alimento funcional. Aun siendo una especie que crece en estado salvaje, sobre suelos forestales; siendo un frutal menor y una planta medicinal su importancia en el consumo masivo debe ser considerada.

  9. Effects of Fruit of Rosa Canina L.Extract on the Level of Plasma Glucose in Male Diabet-Induced Rats

    Directory of Open Access Journals (Sweden)

    A Mohammad Eini

    2016-03-01

    Full Text Available Introduction: Diabetes mellitus is one of the most prevalent chronic and complex metabolic diseases of human , which hyperglycemia can be mentioned as its prominent characteristic. Therefore, this study aimed to evaluate the hypoglycemic effects of fruit of Rosa canina (R.c. extract in healthy and diabetic rats. Method: A total of 72 Wistar male rats were divided into six group: control,  STZ (diabetic control,  R.c. control (50 mg/kg, R.c. control (100 mg/kg and two experimental groups with 50, and 100 mg/kg of extract dose. Diabetes was induced using streptozotocine (60 mg/kg; IP, and blood collection was carried out on 0, 2 and 4 hours after the oral administration of the extracts. Results: The levels of plasma glucose were determined by spectrophotometric method. In order to statistically analyze the study data, ANOVA test was performed. There was a significant difference between groups concerning the plasma glucose concentration (P<0.0001, which the lowest concentration between diabetes groups was observed in the two experimental groups. Moreover, R.c. had a marked hypoglycemic effect on diabetes mellitus. Conclusion: R.c. extract in hyperglycemic status demands to be further studied in order to control and prevent its complications.

  10. To be or not to be the odd one out - Allele-specific transcription in pentaploid dogroses (Rosa L. sect. Caninae (DC. Ser

    Directory of Open Access Journals (Sweden)

    Theißen Günter

    2011-02-01

    Full Text Available Abstract Background Multiple hybridization events gave rise to pentaploid dogroses which can reproduce sexually despite their uneven ploidy level by the unique canina meiosis. Two homologous chromosome sets are involved in bivalent formation and are transmitted by the haploid pollen grains and the tetraploid egg cells. In addition the egg cells contain three sets of univalent chromosomes which are excluded from recombination. In this study we investigated whether differential behavior of chromosomes as bivalents or univalents is reflected by sequence divergence or transcription intensity between homeologous alleles of two single copy genes (LEAFY, cGAPDH and one ribosomal DNA locus (nrITS. Results We detected a maximum number of four different alleles of all investigated loci in pentaploid dogroses and identified the respective allele with two copies, which is presumably located on bivalent forming chromosomes. For the alleles of the ribosomal DNA locus and cGAPDH only slight, if any, differential transcription was determined, whereas the LEAFY alleles with one copy were found to be significantly stronger expressed than the LEAFY allele with two copies. Moreover, we found for the three marker genes that all alleles have been under similar regimes of purifying selection. Conclusions Analyses of both molecular sequence evolution and expression patterns did not support the hypothesis that unique alleles probably located on non-recombining chromosomes are less functional than duplicate alleles presumably located on recombining chromosomes.

  11. Canine hypertrophic osteopathy associated with extra-thoracic lesions Osteopatia hipertrófica canina associada com lesões extratorácicas

    Directory of Open Access Journals (Sweden)

    Selwyn Arlington Headley

    2005-08-01

    Full Text Available Canine hypertrophic osteopathy is described in a dog that presented extra-thoracic lesions, mainly in the liver. Hepatic lesions were characterized by necrosis, hemorrhage, severe hydropic degeneration of centrolobular hepatocytes, proliferation of epithelial cells of bile ducts, and mild biliary stasis. The disease syndrome was diagnosed based on clinical signs, radiological evaluation, and inspection of macerated bones.Osteopatia hipertrófica canina é descrita em um cão que apresentou lesões extratorácicas, principalmente no fígado. As alterações hepáticas foram caracterizadas por necrose, hemorragia, degeneração hepatocelular severa, proliferação das células epiteliais dos ductos biliares e estase biliar. O diagnóstico desta síndrome foi baseado nos sinais clínicos, a avaliação radiológica e a inspeção dos ossos macerados.

  12. Reuniones, dispersiones. Notas sobre ideas literarias de Tomás Segovia

    Directory of Open Access Journals (Sweden)

    Juan Pascual Gay

    2014-01-01

    Full Text Available Este artículo quiere dar cuenta de diferentes ideas expuestas por Tomás Segovia a lo largo de su oficio de escritor en ensayos, cuadernos de notas y entrevistas. Segovia no desarrolló un pensamiento unitario y sistemático, sino que, más bien, desarrolla esas ideas en diferentes contextos, textos y pretextos, pero esas ideas acaban por volver siem - pre a un ideario que otorga una cohesión insospechada en su obra. Además, muchas veces, sin decirlo, deja entrever la tradición literaria y cultural a la que se adscribe y que permea su obra entera.

  13. NOTA DE PESQUISA: AS MÁSCARAS DE PLATÃO E FERNANDO PESSOA

    Directory of Open Access Journals (Sweden)

    Rodolfo Lopes

    2017-06-01

    Full Text Available A proposta de pesquisa brevemente esboçada nesta nota nasceu de um modo tão insólito quanto talvez seja o seu principal objetivo. Durante uma aula que ministrei na ANPOF de 2016 (inserida no Minicurso Platão e Platonismo, em que tive o prazer de colaborar com os colegas e amigos Gabriele Cornelli e Renato Matoso sobre algumas dificuldades metodológicas resultantes do singular registo em que Platão codificou a sua filosofia, e particularmente a propósito da galeria de personagens que lhe vão dando voz(es, surgiu-me a seguinte hipótese: poderá a heteronímia de Fernando Pessoa contribuir, de algum modo, para reconstituir uma 'teoria da personagem' (implícita em Platão?

  14. Notas sobre los visitadores florales de Seemannia sylvatica (Kunth Hanstein (Gesneriaceae

    Directory of Open Access Journals (Sweden)

    Lianka Cairampoma

    2012-10-01

    Full Text Available La polinización en Gesneriaceae comúnmente se asocia a síndromes de ornitofilia o melitofilia, siendo usual además la visita a las flores por especies robadoras de néctar. Se presenta una nota sobre la fauna que visita las flores de Seemannia sylvatica en Cusco, registrándose colibríes e insectos visitando las flores. En base a las características florales y conducta de los visitadores florales se sugiere que las flores de S. sylvatica presentan un síndrome ornitofílico. Se concluye que únicamente los colibríes serian los polinizadores, mientras que los insectos visitadores no intervendrían en la polinización y serian robadores del néctar de las flores.

  15. Challenging the current paradigm of melanoma progression: brain metastasis as isolated first visceral site

    Science.gov (United States)

    Ma, Michelle W.; Qian, Meng; Lackaye, Daniel J.; Berman, Russell S.; Shapiro, Richard L.; Pavlick, Anna C.; Golfinos, John G.; Parker, Erik C.; Darvishian, Farbod; Hernando, Eva; Shao, Yongzhao; Osman, Iman

    2012-01-01

    Melanoma brain metastasis that develops as the isolated first visceral site challenges the current paradigm of tumor progression in which brain metastasis is regarded as the final stage. Here we test the hypothesis that melanoma patients who develop brain metastasis as the isolated first visceral site have distinct clinicopathological features at the time of primary melanoma diagnosis. Cutaneous melanoma patients enrolled in 2 prospectively collected databases were studied (Cohort 1: 1972–1982, Cohort 2: 2002–2009). Patients who developed brain metastasis as isolated first visceral site were compared with (1) all other patients, (2) patients who developed visceral metastasis: extracranial only or extracranial and brain, and (3) patients who progressed to other isolated visceral sites first. Two hundred seven of 2280 (9.1%) patients developed brain metastasis (median follow–up, 5.2 y). Seventy–four of 207 (35.7%) brain metastasis patients progressed to brain metastasis as the isolated first visceral site. These patients presented with primaries that were thinner and had no mitosis compared with all other visceral metastasis patients (Fisher's combined P = .02, .05, respectively), and there was a significant difference in American Joint Committee on Cancer stage distribution at initial melanoma diagnosis (combined P = .02). Post–visceral metastasis survival, however, was shorter in patients with brain metastasis as isolated first visceral site than in patients with visceral metastasis: extracranial and brain (combined P = .03). Brain metastasis as isolated first visceral site is a distinct clinicopathological entity. Studies are needed to better understand the biological factors driving this phenotype at the time of primary melanoma diagnosis and to determine its clinical implications. PMID:22561799

  16. Development and evaluation of agents for targeting visceral amyloid.

    Science.gov (United States)

    Wall, Jonathan S; Solomon, Alan; Kennel, Stephen J

    2011-12-01

    Wall, JS, Solomon A, Kennel, SJ. Development and evaluation of agents for targeting visceral amyloid. Visceral amyloidosis is a rare disease characterized by the deposition in organs and tissues of protein fibrils, heparan sulfate proteoglycan as well as serum amyloid P component and other serum proteins. Imaging these pathologic deposits aids in the clinical management of patients with amyloidosis. Whole body scintigraphic imaging of amyloid load as well as organ specific anatomic imaging provides information that can inform prognosis and can be used to monitor disease progression or response to therapy. These capabilities are limited in the USA, which has led to our development and evaluation of two new reagents that specifically target amyloid in vivo and have been used to image visceral deposits in mice and patients with AL amyloidosis. The fibril-reactive mAb 11-1F4, when labeled with iodine-124 was shown to bind AL amyloid in patients by using PET/CT imaging. These studies were performed to support the evaluation of this reagent as a novel immunotherapy for AL patients. In addition, we have identified a heparin-binding peptide that co-localizes with murine AA amyloid in vivo and can be used to image the deposits. The interaction of this peptide, designated p5, with amyloid is dependent on the net positive charge and truncated forms that would be more desirable as clinical imaging agents were found to be significantly less efficient for amyloid imaging. The development and positive preclinical validation of these two reagents offers potential new therapeutic and diagnostic tools for patients with these devastating diseases.

  17. Blood pressure and renal injury in dogs with visceral leishmaniasis

    Directory of Open Access Journals (Sweden)

    Marlos G. Sousa

    Full Text Available Abstract: Systemic hypertension is known to be a common consequence of chronic renal disease, which is frequently diagnosed in dogs with visceral leishmaniasis. Although many veterinary investigations have looked at the renal injury caused by Leishmania spp., the role played by this complication in the development of arterial hypertension documented in some animals with visceral leishmaniasis is not completely understood. In this study, 18 adult dogs with naturally-occurring visceral leishmaniasis and varying clinical signs underwent an indirect blood pressure measurement. Also, sera and spot urine were used for laboratory tests. The median systolic blood pressure was 135.2mmHg (95% confidence interval: 128.5-147.7, median mean arterial pressure was 105.8mmHg (98.3-110.4, and median diastolic arterial pressure was 88.5mmHg (77.8-92.5. No differences existed between asymptomatic and symptomatic animals regarding arterial pressure, and no correlations were documented between blood pressure and serum creatinine, blood urea, urine protein-to-creatinine ratio, urine specific gravity, and the fractional excretion of sodium and potassium. Although an association between hypertension and the identification of inflammation on histopathology could not be demonstrated in hypertensive animals, the assessment of kidney samples from 12 dogs indicated mild inflammation with a lymphoplasmacytic infiltrate (6/12, moderate inflammation with multifocal lymphoplasmacytic and histiocytic infiltrates (3/12, and multifocal degeneration and protein casts (2/12. Anti-Leishmania spp. immunohistochemistry assays stained the renal epithelium in 2/12 of the animals. Even though mild systemic hypertension was documented in a small subset of animals, no relationship between the severity of clinical signs and hypertension could be anticipated.

  18. Visceral Malperfusion in Aortic Dissection: The Michigan Experience.

    Science.gov (United States)

    Kamman, Arnoud V; Yang, Bo; Kim, Karen M; Williams, David M; Michael Deeb, George; Patel, Himanshu J

    2017-01-01

    One of the most dreaded complications of acute aortic dissection is end-organ malperfusion. We summarize current evidence and describe our treatment paradigm in the setting of malperfusion in aortic dissection. Given the difficulty with identifying isolated visceral malperfusion in aortic dissection, both in the literature as well as in our practice, we have broadened the discussion to include data examining the presentation complex of malperfusion, particularly if mesenteric ischemia is identified. The approach to treating malperfusion syndrome is different depending on whether the patient presents with type A dissection vs type B dissection with malperfusion. Although thoracic endovascular aortic repair has emerged as the dominant strategy for resolving malperfusion for complicated type B dissection, fenestration may still have a role in its treatment. In contrast, for type A aortic dissection presenting with visceral malperfusion, the concept of operative repair after restoration of end-organ perfusion has been proposed with increasing frequency in recent reports. At the University of Michigan, we apply a patient-specific algorithm, based on the presence of malperfusion with end-organ dysfunction. In those patients presenting with visceral malperfusion, we prefer to first fenestrate, await resolution of the malperfusion syndrome and then perform central aortic repair. We recognize that other groups have implemented similar algorithms to reduce the dismal results of operative procedures in this cohort. However, the most appropriate period of delay remains unknown and there is a persistent risk of rupture before repair is performed. Future studies should be performed to determine whether these various treatment paradigms have merit. Copyright © 2016 Elsevier Inc. All rights reserved.

  19. Accounting for False Positive HIV Tests: Is Visceral Leishmaniasis Responsible?

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    Leslie Shanks

    Full Text Available Co-infection with HIV and visceral leishmaniasis is an important consideration in treatment of either disease in endemic areas. Diagnosis of HIV in resource-limited settings relies on rapid diagnostic tests used together in an algorithm. A limitation of the HIV diagnostic algorithm is that it is vulnerable to falsely positive reactions due to cross reactivity. It has been postulated that visceral leishmaniasis (VL infection can increase this risk of false positive HIV results. This cross sectional study compared the risk of false positive HIV results in VL patients with non-VL individuals.Participants were recruited from 2 sites in Ethiopia. The Ethiopian algorithm of a tiebreaker using 3 rapid diagnostic tests (RDTs was used to test for HIV. The gold standard test was the Western Blot, with indeterminate results resolved by PCR testing. Every RDT screen positive individual was included for testing with the gold standard along with 10% of all negatives. The final analysis included 89 VL and 405 non-VL patients. HIV prevalence was found to be 12.8% (47/ 367 in the VL group compared to 7.9% (200/2526 in the non-VL group. The RDT algorithm in the VL group yielded 47 positives, 4 false positives, and 38 negatives. The same algorithm for those without VL had 200 positives, 14 false positives, and 191 negatives. Specificity and positive predictive value for the group with VL was less than the non-VL group; however, the difference was not found to be significant (p = 0.52 and p = 0.76, respectively.The test algorithm yielded a high number of HIV false positive results. However, we were unable to demonstrate a significant difference between groups with and without VL disease. This suggests that the presence of endemic visceral leishmaniasis alone cannot account for the high number of false positive HIV results in our study.

  20. Accounting for False Positive HIV Tests: Is Visceral Leishmaniasis Responsible?

    Science.gov (United States)

    Shanks, Leslie; Ritmeijer, Koert; Piriou, Erwan; Siddiqui, M Ruby; Kliescikova, Jarmila; Pearce, Neil; Ariti, Cono; Muluneh, Libsework; Masiga, Johnson; Abebe, Almaz

    2015-01-01

    Co-infection with HIV and visceral leishmaniasis is an important consideration in treatment of either disease in endemic areas. Diagnosis of HIV in resource-limited settings relies on rapid diagnostic tests used together in an algorithm. A limitation of the HIV diagnostic algorithm is that it is vulnerable to falsely positive reactions due to cross reactivity. It has been postulated that visceral leishmaniasis (VL) infection can increase this risk of false positive HIV results. This cross sectional study compared the risk of false positive HIV results in VL patients with non-VL individuals. Participants were recruited from 2 sites in Ethiopia. The Ethiopian algorithm of a tiebreaker using 3 rapid diagnostic tests (RDTs) was used to test for HIV. The gold standard test was the Western Blot, with indeterminate results resolved by PCR testing. Every RDT screen positive individual was included for testing with the gold standard along with 10% of all negatives. The final analysis included 89 VL and 405 non-VL patients. HIV prevalence was found to be 12.8% (47/ 367) in the VL group compared to 7.9% (200/2526) in the non-VL group. The RDT algorithm in the VL group yielded 47 positives, 4 false positives, and 38 negatives. The same algorithm for those without VL had 200 positives, 14 false positives, and 191 negatives. Specificity and positive predictive value for the group with VL was less than the non-VL group; however, the difference was not found to be significant (p = 0.52 and p = 0.76, respectively). The test algorithm yielded a high number of HIV false positive results. However, we were unable to demonstrate a significant difference between groups with and without VL disease. This suggests that the presence of endemic visceral leishmaniasis alone cannot account for the high number of false positive HIV results in our study.

  1. Visceral leishmaniasis and leishmaniasis-HIV coinfection: comparative study

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    João Victor Soares Coriolano Coutinho

    Full Text Available Abstract INTRODUCTION: This study aimed to draw clinical and epidemiological comparisons between visceral leishmaniasis (VL and VL associated with human immunodeficiency virus (HIV infection. METHOD: Retrospective study. RESULTS: Of 473 cases of VL, 5.5% were coinfected with HIV. The highest proportion of cases of both VL and VL/HIV were found among men. A higher proportion of VL cases was seen in children aged 0-10 years, whereas coinfection was more common in those aged 18-50 years. CONCLUSIONS: VL/HIV coinfected patients presented slightly differently to and had a higher mortality rate than those with VL only.

  2. Visceral leishmaniasis: immunology and prospects for a vaccine.

    Science.gov (United States)

    Kaye, P M; Aebischer, T

    2011-10-01

    Human visceral leishmaniasis (HVL) is the most severe clinical form of a spectrum of neglected tropical diseases caused by protozoan parasites of the genus Leishmania. Caused mainly by L. donovani and L. infantum/chagasi, HVL accounts for more than 50 000 deaths every year. Drug therapy is available but costly, and resistance against several drug classes has evolved. Here, we review our current understanding of the immunology of HVL and approaches to and the status of vaccine development against this disease. © 2011 The Authors. Clinical Microbiology and Infection © 2011 European Society of Clinical Microbiology and Infectious Diseases.

  3. Trace elements in sera from patients with visceral leishmaniasis

    Energy Technology Data Exchange (ETDEWEB)

    Mukhopadhyay, R.; Bhattacharya, A. [Department of Zoology, Calcutta University, Calcutta (India); Chakraborty, A.; Sudarshan, M.; Jal, P.K.; Chintalapudi, S.N. [Inter University Consortium for DAE Facilities, Calcutta Centre 3/LB-8, Bidhan Nagar, Calcutta (India); Dutta, R.K. [Schonland Research Centre for Nuclear Sciences, University of Witwatersrand, Johannesburg (South Africa)

    2000-07-01

    Trace elements are known to have pivotal role in human health and disease. Present investigation employed PIXE analysis to probe into the elemental profile of patients suffering from visceral Leishmaniasis. Remarkable alternations were observed in concentration of elements like Cl, K, Ca, Mn, Fe, Cu, Zn. The pattern of enhancement of elemental concentration corresponds to the progression of the disease. Additionally, our present data reflect probable correlation between alteration in trace elemental status and other pathological syndromes associated with Leishmaniasis. The possibility of considering trace elements as a diagnostic marker for a better understanding of the disease is discussed. (author)

  4. Longitudinal variance of visceral fat thickness in pregnant adolescents.

    Science.gov (United States)

    Dutra, Luciana P; Cisneiros, Rosangela M; Souza, Alex S; Diniz, Carolina P; Moura, Laís A; Figueiroa, Jose N; Alves, João G B

    2014-02-01

    This study aims to investigate the longitudinal change in visceral fat thickness (VFT) during normal pregnancy. A prospective cohort study with 75 primiparous adolescents was carried out in Petrolina, Brazil. VFT was evaluated by ultrasound between 12-20 weeks gestation and immediately after delivery. We noted a statistically significant increase in VFT; 1.3 cm ± 1.0. No correlation was found between VFT and maternal anthropometric variables. VFT increases about 30% from the first to the second half of pregnancy in primiparous adolescents. © 2014 The Royal Australian and New Zealand College of Obstetricians and Gynaecologists.

  5. AMTB CHILE. INFORME TÉCNICO INACAP TEMUCO 2008: MOTIVACIÓN Y NOTAS EN INGLÉS

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    María Soledad Soza Roldán

    2013-01-01

    Full Text Available La siguiente investigación trata la motivación como factor que impacta en el aprendizaje de inglés (notas en estudiantes de pregrado de Inacap Temuco, Región de la Araucanía, Chile. Para este fin, se aplicó el cuestionario AMTB (Test de Actitud y Motivación a una muestra de 305 estudiantes de carreras técnicas en Inacap Temuco (2008 para determinar el nivel de motivación según 12 variables del modelo socio-educacional de R C Gardner que sirve de paradigma en SLA (adquisición de un segundo idioma. Los hallazgos indican que la motivación explica el 41% de la nota; la ansiedad, deseo por aprender Inglés, intensidad de la motivación, el estímulo de los padres, y las actitudes frente al proceso de enseñanza tienen correlación significativa con las notas (95% de confianza. Se realizó un análisis descriptivo de las notas en Inglés desde otoño /primavera 2002 a otoño/primavera 2007 por nivel y promedio total (nivel I a VII. Se determinó que nivel promedio general de motivación es de un moderado 4.3 (escala Likert de 1 a 6 pero se dispara a 5.9 en el nivel VII permitiendo apreciar una correlación serial positiva desde el nivel I al VII. Los hallazgos son similares a los descubiertos por Gardner en Canadá (1960 y en 4 países europeos (1985. Los hallazgos fueron enviados a Gardner cuyos comentarios se detallan en el Informe.

  6. Trabajar los géneros discursivos en clase de E/LE: la nota crítica

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    Eduardo España Palop

    2013-07-01

    Full Text Available El trabajo con géneros discursivos es esencial para adquirir una correctacompetencia comunicativa. Mediante la secuencia didáctica que aquí se presenta, losalumnos adquirirán las principales herramientas para construir notas críticas, a la vezque trabajarán aspectos de la lengua española que les permitirán incrementar sucompetencia lectora y escrita.

  7. Libros grandes, libros pequeños : una nota sobre las lecturas épicas de Alonso Quijano

    OpenAIRE

    Vilà Tomàs, Lara

    2011-01-01

    Nota sobre el capítulo del escrutinio y la censura de la biblioteca de don Quijote. Se propone cómo su división en dos tipos de libros plantea una dicotomía sobre dos formas opuestas de narrar hechos de guerra. Note on the division and censorship of don Quijote's library and how it shows a dicotomy between two ways of writing military deeds.

  8. Immunoactivation and immunopathogeny during active visceral leishmaniasis Imunoativação e imunopatogenia durante leishmaniose visceral ativa

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    Hiro Goto

    2009-10-01

    Full Text Available Visceral leishmaniasis is caused by protozoan parasites of the Leishmania donovani complex. During active disease in humans, high levels of IFN-γ and TNF-α detected in blood serum, and high expression of IFN-γ mRNA in samples of the lymphoid organs suggest that the immune system is highly activated. However, studies using peripheral blood mononuclear cells have found immunosuppression specific to Leishmania antigens; this poor immune response probably results from Leishmania antigen-engaged lymphocytes being trapped in the lymphoid organs. To allow the parasites to multiply, deactivating cytokines IL-10 and TGF-β may be acting on macrophages as well as anti-Leishmania antibodies that opsonize amastigotes and induce IL-10 production in macrophages. These high activation and deactivation processes are likely to occur mainly in the spleen and liver and can be confirmed through the examination of organ samples. However, an analysis of sequential data from studies of visceral leishmaniasis in hamsters suggests that factors outside of the immune system are responsible for the early inactivation of inducible nitric oxide synthase, which occurs before the expression of deactivating cytokines. In active visceral leishmaniasis, the immune system actively participates in non-lymphoid organ lesioning. While current views only consider immunocomplex deposition, macrophages, T cells, cytokines, and immunoglobulins by diverse mechanism also play important roles in the pathogenesis.A leishmaniose visceral é causada por protozoários do gênero do complexo Leishmania donovani. Durante a doença ativa no homem são detectados altos níveis de IFN-γ e de TNF-α no soro, e elevada expressão de mRNA de IFN-γ em amostras de órgãos linfóides sugerindo um estado intensamente ativado do sistema imunológico. A visão atual, no entanto, refere-se à imunossupressão específica aos antígenos de Leishmania com base em estudos utilizando células mononucleares

  9. Aspectos epidemiológicos da dirofilariose canina no perímetro urbano de Cuiabá, Mato Grosso: emprego do "Immunoblot" e do teste de Knott modificado

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    Cláudia Gorgulho Nogueira Fernandes

    2000-12-01

    Full Text Available O presente trabalho refere-se a pesquisa dos aspectos epidemiológicos da dirofilariose canina no perímetro urbano de Cuiabá, através de exame de sangue e sorologia. Examinou-se o sangue de 822 cães: em 11,81% das amostras, detectaram-se, por meio de "Immunoblot", anticorpos contra antígenos de Dirofilaria immitis adulto, enquanto em 0,41% foram encontradas, por meio do teste de Knott modificado, microfilárias de D. immitis. A prevalência da dirofilariose canina em Cuiabá, no período de junho a novembro de 1997, e com a metodologia aplicada, foi de 120,8 casos em cada 1.000 animais. O "Immunoblot" diagnosticou como positivas 11,27% das amostras que estavam consideradas negativas pelo teste de Knott modificado. Os autores analisaram estatisticamente informações sobre raça, sexo, idade e região administrativa de origem dos cães pesquisados. Foi questionada a possibilidade de que os animais pesquisados tivessem viajado a regiões endêmicas, usado microfilaricidas, ou apresentado sintomatologia compatível, bem como estudadas as características ecológicas do local de moradia. Cães com idade variando entre um e nove anos e residentes na região Centro-Oeste apresentaram maior índice de positividade. A maioria não apresentava sintomatologia compatível com a doença, não tinha história clínica do uso de microfilaricidas e/ou preventivos contra dirofilariose canina e nunca havia saído do perímetro urbano de Cuiabá, mas residiam em bairros próximos a rios ou córregos e/ou em casas contíguas a áreas de vegetação nativa. Pela primeira vez, o "Immunoblot" foi empregado no diagnóstico de dirofilariose canina no Brasil.

  10. Synthesis of (68)Ga-labeled NOTA-RGD-GE11 heterodimeric peptide for dual integrin and epidermal growth factor receptor-targeted tumor imaging.

    Science.gov (United States)

    Yu, Hung-Man; Chen, Jyun-Hong; Lin, Kun-Liang; Lin, Wuu-Jyh

    2015-06-15

    Radiolabeled Arg-Gly-Asp (RGD) peptide analogs have been extensively studied for αvβ3 integrin-targeted angiogenesis imaging. According to recently presented evidence, the dodecapeptide GE11 has high affinity to the epidermal growth factor receptor (EGFR), which is overexpressed in many types of cancer. Dual-receptor molecular imaging probes with two different heterodimeric peptides exhibit improved cancer targeting efficacy. In the present study, the design and synthesis of a new RGD-GE11 peptide heterodimer for dual αvβ3 integrin/EGFR-targeted cancer imaging are described. The RGD-GE11 heterodimer was linked with 6-aminohexanoic acid (6-Ahx) and cysteine and conjugated with 1,4,7-triazacyclononane-N,N',N″-triacetic acid (NOTA) to form NOTA-RGD-cys-6-Ahx-GE11. The monomeric peptides, NOTA-cys-6-Ahx-GE11 and c(RGDyK), were formed by a peptide synthesizer. The peptide heterodimer NOTA-RGD-GE11 was obtained by NOTA-cys-6-Ahx-GE11 and maleimidopropyl-c(RGDyK) conjugation with a thioether linkage. The NOTA peptide conjugate was labeled with freshly eluted (68)Ga and purified using reversed-phase high-performance liquid chromatography. The (68)Ga-NOTA-RGD-cys-6-Ahx-GE11 was successfully prepared, in this study, with a radiochemical yield of 85% and a radiochemical purity of >98%. These results warrant further investigation of this heterodimeric peptide's binding affinity to the receptors. Copyright © 2015 John Wiley & Sons, Ltd.

  11. Autotomy of the Visceral mass in the feather star Himerometra robustipinna (Crinoidea, Comatulida).

    Science.gov (United States)

    Bobrovskaya, Nadezhda V; Dolmatov, Igor Yu

    2014-04-01

    The microanatomy of the attachment sites of the visceral mass to the calyx before and after visceral mass autotomy in the feather star Himerometra robustipinna was investigated. At the aboral site, the visceral mass is linked to the calyx by septa of the aboral coelom and is attached to the tegmen at the peripheral site. The connective tissue of the septa and tegmen contains cells resembling typical juxtaligamental cells of echinoderms, nerve cells, and bundles of axons. Visceral mass autotomy in H. robustipinna can be provoked by mechanical action and occurs relatively rapidly. Immediately after the visceral mass is gripped with forceps, the proximal pinnules are lowered and form a dense cluster covering the calyx. If the visceral mass is held for 20-30 s, the proximal pinnules are raised. At this time, the visceral mass has separated from the calyx and can be easily removed. During autotomy, the aboral coelomic septa are broken under the aboral wall of the subintestinal coelom, and the tegmen is ruptured at the interradii along the periphery of the calyx and at the base of the arms. The juxtaligamental cells probably participate in the alteration of the connective tissue and the breakage of the septa and tegmen. The granules of juxtaligamental cells swell, develop an electron-transparent halo, and are released into the extracellular matrix. In general, our results suggest that separation of the visceral mass in H. robustipinna is characterized by all the features of autotomy.

  12. Long term testosterone administration increases visceral fat mass in female-to-male transsexuals

    NARCIS (Netherlands)

    Elbers, J.M.H.; Asscheman, H.; Seidell, J.C.; Megens, J.A.J.; Gooren, L.J.G.

    1997-01-01

    The amount of intraabdominal (visceral) fat is an important determinant of disturbances in lipid and glucose metabolism. Cross-sectional studies in women have found associations between high androgen levels and visceral fat accumulation. The causal relation between these phenomena is unknown. We,

  13. Long-term testosterone administration increases visceral fat in female to male transsexuals

    NARCIS (Netherlands)

    Elbers, J.M.; Asscheman, H.; Seidell, J C; Megens, J.A.J.; Gooren, Louis J G

    The amount of intraabdominal (visceral) fat is an important determinant of disturbances in lipid and glucose metabolism. Cross-sectional studies in women have found associations between high androgen levels and visceral fat accumulation. The causal relation between these phenomena is unknown. We,

  14. Stress-induced visceral pain: toward animal models of irritable-bowel syndrome and associated comorbidities.

    Science.gov (United States)

    Moloney, Rachel D; O'Mahony, Siobhain M; Dinan, Timothy G; Cryan, John F

    2015-01-01

    Visceral pain is a global term used to describe pain originating from the internal organs, which is distinct from somatic pain. It is a hallmark of functional gastrointestinal disorders such as irritable-bowel syndrome (IBS). Currently, the treatment strategies targeting visceral pain are unsatisfactory, with development of novel therapeutics hindered by a lack of detailed knowledge of the underlying mechanisms. Stress has long been implicated in the pathophysiology of visceral pain in both preclinical and clinical studies. Here, we discuss the complex etiology of visceral pain reviewing our current understanding in the context of the role of stress, gender, gut microbiota alterations, and immune functioning. Furthermore, we review the role of glutamate, GABA, and epigenetic mechanisms as possible therapeutic strategies for the treatment of visceral pain for which there is an unmet medical need. Moreover, we discuss the most widely described rodent models used to model visceral pain in the preclinical setting. The theory behind, and application of, animal models is key for both the understanding of underlying mechanisms and design of future therapeutic interventions. Taken together, it is apparent that stress-induced visceral pain and its psychiatric comorbidities, as typified by IBS, has a multifaceted etiology. Moreover, treatment strategies still lag far behind when compared to other pain modalities. The development of novel, effective, and specific therapeutics for the treatment of visceral pain has never been more pertinent.

  15. Genome-wide association for abdominal subcutaneous and visceral adipose reveals a novel locus for visceral fat in women.

    Directory of Open Access Journals (Sweden)

    Caroline S Fox

    Full Text Available Body fat distribution, particularly centralized obesity, is associated with metabolic risk above and beyond total adiposity. We performed genome-wide association of abdominal adipose depots quantified using computed tomography (CT to uncover novel loci for body fat distribution among participants of European ancestry. Subcutaneous and visceral fat were quantified in 5,560 women and 4,997 men from 4 population-based studies. Genome-wide genotyping was performed using standard arrays and imputed to ~2.5 million Hapmap SNPs. Each study performed a genome-wide association analysis of subcutaneous adipose tissue (SAT, visceral adipose tissue (VAT, VAT adjusted for body mass index, and VAT/SAT ratio (a metric of the propensity to store fat viscerally as compared to subcutaneously in the overall sample and in women and men separately. A weighted z-score meta-analysis was conducted. For the VAT/SAT ratio, our most significant p-value was rs11118316 at LYPLAL1 gene (p = 3.1 × 10E-09, previously identified in association with waist-hip ratio. For SAT, the most significant SNP was in the FTO gene (p = 5.9 × 10E-08. Given the known gender differences in body fat distribution, we performed sex-specific analyses. Our most significant finding was for VAT in women, rs1659258 near THNSL2 (p = 1.6 × 10-08, but not men (p = 0.75. Validation of this SNP in the GIANT consortium data demonstrated a similar sex-specific pattern, with observed significance in women (p = 0.006 but not men (p = 0.24 for BMI and waist circumference (p = 0.04 [women], p = 0.49 [men]. Finally, we interrogated our data for the 14 recently published loci for body fat distribution (measured by waist-hip ratio adjusted for BMI; associations were observed at 7 of these loci. In contrast, we observed associations at only 7/32 loci previously identified in association with BMI; the majority of overlap was observed with SAT. Genome-wide association for visceral and subcutaneous fat revealed a

  16. Nota editorial

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    Geofísica Colombiana

    2000-08-01

    Full Text Available

    Cada nuevo día que estemos muertos, dificultara más nuestro renacer. Fue un verdadero reto seguir y esperamos que este haya sido nuestro último renacer; Geofísica Colombiana ha vuelto, con cambios y con la intención de perdurar, además de servir. El número cuatro de nuestra revista aparece ligado a la Universidad Nacional de Colombia, Facultad de Ciencias, financiado por el Programa de Posgrado en Geofísica, respaldado por el Grupo de Geofísica (escalafonado por Colciencias como Grupo de Investigación en 1999, con el propósito de colaborar en la difusión de la investigación y aplicación de temas conexos con la Geofísica, a nivel nacional e internacional, pero en particular apoyando el trabajo que la Maestría de nuestro Programa habrá de realizar, canalizando esfuerzos para contribuir al desarrollo de las Geociencias nacionales.

  17. Nota Gotica

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    Renato Gendre

    2002-12-01

    Full Text Available Dalla documentazione presentata, ancorché in modo non completo, si può constatare che ogni qual volta il testo greco presenta i1 verbo nella posizione 'in incastro', 2 anche in quello gotico troviamo la stessa situazione. E benché crediamo a una sostanziale dipendenza dal greco dalla prassi sintattica gotica, 3 tuttavia riteniamo che non si possa del tutto escludere di trovarsi in presenza di uno stesso tratto sintattico, cioé di uno stesso modo di distribuire l'informazione, secondo un preciso ordine dei costituenti di frase, di origine indoeuropea. La "spezzatura di ciò che secondo il nostro sentimento linguistico è unito [ ... ] è comune sia tra i Greci che tra i Latini e gli Indiani4 e si trova in tracce ben riconoscibili anche nell'epica germanica".5 Purtroppo, come già G. Bonfante, anche noi pur "scandagliando l'epopea germanica [non abbiamo] trovato nulla in questo senso".6 La presenza sicura di questo tratto nel gotico però e, per chi come noi gli dà valore, l 'uso della tmesi 7 in testi epici germanici 8 sono 1í ad avvalorare l'ipotesi che la posizione 'in incastro' del verbo rappresenta "il modo più antico, facilmente c omprensibile dal p unto di v ista psicologico, di ordinare le paro le n ella frase idg". 9 È ben vero che il passo del Vangelo di Luca (2, 25 "7tveuμa. Tiv &ytov E7t' a.u't6v", reso in gotico "ahma weihs was ana imma", sembrerebbe opporsi a quanto è stato appena affermato. Ma cosí non è. Infatti, "il ne faut pas perdre de vue que Wulfila a suivi un manuscrit grec du type de *K ou *Kt, mais que la version gotique présente des leçons propre à 1cxo5" .1 O E molti manoscritti appartenenti al 'Ti po I' 11 riportano la lezione "7tveuμa. &ytov Tiv". 12 Pertanto, l'unica conclusione che da questo esempio si deve trarre è che il grande Vescovo dei Goti abbia avuto sotto gli occhi un testo di questa ultima famiglia e, come è nel suo stile, ne sia stato condizionato.

  18. NOTA EDITORIAL

    Directory of Open Access Journals (Sweden)

    Mario Camacho Pinto

    1985-08-01

    Full Text Available

    Vigencia de la tradición.

    A propósito del cambio en la presentación de la portada de MEDICINA hacemos un comentario de tipo periodístico, ya que afrontamos varios aspectos que tanto por las Directivas de la Academia como por el Consejo Editorial fueron cuidadosamente analizados antes de tomar esta determinación. Sea el primero de ellos el de buscar para la Revista una identidad fiel y estable, trasunto representativo de su función académica de informar sobre la actividad que se mueve en su seno y que comprende las inquietudes de sus Directivas y miembros, traducidas en iniciativas y trabajos científicos propios u ocasionalmente también solicitados o aceptados a colaboradores notables, con la finalidad de estimular la calidad, altura y variedad para nuestra publicación.

    Otro aspecto de esa identidad no atañe el patrimonio científico y cultural, sino a la presentación, puesto que se consideró en las deliberaciones que la revista de la Academia Nacional de Medicina debe mostrar definidamente algo de la majestad inherente a su categoría auténticamente nacional que exige culto a la tradición ecuménica representada en este caso por el emblema de Esculapio y a la vez a la historia vernácula representada simbólicamente aquí. En primer lugar, por MEDICINA que es la continuidad de la original REVISTA MEDICA existente en el siglo pasado, a la sazón publicación periódica de la sociedad de MEDICINA Y CIENCIAS NATURALES cuando fue promulgada la Ley 71 de 1890 que la convirtió, por acuerdo y voluntad del Gobierno Nacional de esa época y de los siguientes, en ACADEMIA NACIONAL DE MEDICINA DE COLOMBIA Y cuyo artículo 70. reza así..."LA REVISTA MEDICA será el periódico oficial de la Academia"; y en segundo lugar por la diagramación interior ilustrada ahora con diseños de nuestro arte precolombino, cultura Tumaco para esta edición.

    Más aspectos comentables son los referibles a la en algo o en mucho ingenua aspiración de que MEDICINA pueda abrirse paso hacia el resto del mundo médico, poco a poco en busca de un equilibrio adecuado a nuestra contextura potencial y a nuestra vocación democrática. Esta por ahora imaginativa prospección, tiene algún fundamento en la compartida decisión de nuestro Presidente de elevar el tiraje a 8.000 ejemplares cuatro veces al año, sus páginas a un número promedio de 70, distribución internacional por medio de los consulados o embajadas y dos nuevas secciones de orden informativo y consistentes la una en resúmenes actualizados de los recientes adelantos médicos mundiales gracias a la colaboración de la Biblioteca Nacional de Washington, a través de un terminal de cumputador, y la otra sobre actividades nacionales en el ramo de la Salud por un acuerdo con el Ministerio.

    Acogimos el emblema de Esculapio porque se compagina mejor con lo anteriormente esbozado, ya que la más vistosa alternativa del Caduceo o heraldo, del bastón con dos alas extendidas y dos serpientes entrelazadas, fue símbolo de mercaderes en el Siglo XVI y solamente en 1856 el Hospital de la Marina de U.S.A, antecesor de la salubridad oficial actual, consideró que el Caduceo sería un buen sím bolo para significar el carácter no combatiente de la clase médica, aceptándose en 1902 por el cuerpo médico de ese país en sustitución de la cruz de San Juan. En cambio el emblema de Esculapio que adorna nuestra portada, fue médico desde la antigüedad habiendo hecho aparición unos 800 años a. de J.C. como símbolo del dios de la Medicina, Asklepio para los griegos y Aesculapius para los romanos. Actualmente es emblema del cuerpo médico del ejército de muchos países y de corporaciones civiles mayores como la American Medical Association 1912 y la Organización Mundial de la Salud desde su fundación en 1947.

    MEDICINA renueva la formal invitación a todos los Académicos a colaborar sin reservas, unidos para beneficio común.

  19. Nota editorial

    OpenAIRE

    Geofísica, Colombiana

    2012-01-01

    Cada nuevo día que estemos muertos, dificultara más nuestro renacer. Fue un verdadero reto seguir y esperamos que este haya sido nuestro último renacer; Geofísica Colombiana ha vuelto, con cambios y con la intención de perdurar, además de servir. El número cuatro de nuestra revista aparece ligado a la Universidad Nacional de Colombia, Facultad de Ciencias, financiado por el Programa de Posgrado en Geofísica, respaldado por el Grupo de Geofísica (escalafonado por Colciencias como Grupo de Inve...

  20. NOTA EDITORIAL

    Directory of Open Access Journals (Sweden)

    Armando Cuesta Santos

    2011-03-01

    Full Text Available Este nuevo número de la revista está dedicado esencialmente al diseño, en sus diferentes manifestaciones. Comprende diseños para el desarrollo del diagnóstico, el establecimiento de métodos, y de sistemas, asociados aquí indistintamente a dos vertientes fundamentales: sistemas de calidad y de gestión de la seguridad y la salud. Se inicia con un artículo sobre el diagnóstico del clima laboral en una empresa de la industria médico farmacéutica, teniendo por objetivo la determinación de las variables que inciden negativamente sobre la percepción acerca de la calidad del trabajo que realizan las personas y la actitud que asumen al respecto. Un segundo trabajo trata la ergonomía y la simulación aplicadas a la industria, con la finalidad de mostrar el impacto de los rediseños ergonómicos realizados a estaciones de trabajo. En un tercer artículo se expone una interpretación, a través de una metodología en los proyectos de desarrollo de software, del requisito ISO para el diseño.

  1. NOTA EDITORIAL

    Directory of Open Access Journals (Sweden)

    Armando Cuesta Santos

    2010-11-01

    Full Text Available

    En el primer artículo de este nuevo número de la revista Ingeniería Industrial, la investigación conduce a realizar un análisis previo a la optimización concreta de problemas reales de enrutamiento. En el siguiente se refleja una investigación relativa a los sistemas de gestión del capital humano por competencias en empresas de mantenimiento; y en el artículo que continúa, se establecen las mejores alternativas sobre las cuales debe basarse la decisión, en un proyecto dirigido a dar respuesta al creciente mercado de neumáticos.

  2. Nota editorial

    OpenAIRE

    Nestor Varela

    2014-01-01

    Se cumplen 10 años desde la fundación de la Asociación de Veterinarios de Vida Silvestre (VVS), también se cumple ya una década en la publicación de las Memorias de la CIMA, uno de los principales órganos dedicados a la divulgación de revisiones, estudios e investigaciones sobre fauna silvestre en Colombia. Ha pasado tiempo desde la concepción de esta revista como un espacio para que estudiantes y profesionales relacionados con la vida silvestre pudiesen compartir sus experiencias y o...

  3. Nota Editorial

    Directory of Open Access Journals (Sweden)

    Consejo Editorial Maestro y Sociedad

    2016-11-01

    Full Text Available La Revista Maestro y Sociedad de la Universidad de Oriente ha decidido publicar este segundo número especial, donde se incluyen los artículos más destacados presentados en el III Taller Científico “Orientación educativa y desarrollo humano”, que se desarrolló en mayo del 2016 por el Centro de Estudios Pedagógicos Juan Bautista Sagarra Blez de la Facultad de Educación Infantil en la Universidad de Oriente. En este evento se socializaron resultados de investigaciones educativas, así como experiencias científico-metodológicas y pedagógicas desarrolladas por la comunidad científica de esta institución y del territorio, relacionadas con el papel de la orientación educativa como función esencial de los educadores en la formación y desarrollo de la personalidad de niños, adolescentes, jóvenes y adultos, en relación con las transformaciones y exigencias de la contemporaneidad.

  4. NOTA EDITORIAL

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    Armando Cuesta Santos

    2008-11-01

    Full Text Available

    Se promulgó a fines del año 2007 el Decreto Ley No. 252 “Sobre la Continuidad y el Fortalecimiento del Sistema de Dirección y Gestión Empresarial Cubano”, que significa la continuidad del Proceso de Perfeccionamiento Empresarial en Cuba. Su impacto, que es estratégico para nuestra gestión empresarial, también lo es para el accionar de especialistas, investigadores, profesores, y profesionales en general dedicados a la gestión empresarial en el país.

  5. NOTA EDITORIAL

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    Armando Cuesta Santos

    2009-05-01

    Full Text Available

    Los procesos de trabajo atraviesan a todas las funciones de la gestión empresarial u organizacional en esta contemporaneidad. Hoy son la expresión mayor de la transdisciplinariedad comprendida por la actividad científica que es la Ingeniería Industrial, que desde su génesis se manifestara con un enfoque interdisciplinario mediante los estudios de métodos y tiempos de trabajo que realizara F. W. Taylor conformando su Scientific Management.

  6. Nota Editorial

    OpenAIRE

    Galinha,Iolanda Costa

    2013-01-01

    A Direcção e a Coordenação Editorial da revista Psique têm o prazer de informar os seus leitores que o volume IX da revista Psique foi enviado para paginação em Julho de 2013. Conseguindo antecipar a data de fecho deste volume, cumprimos um dos principais objectivos para este ano e que representa encurtar o caminho que leva a Psique à indexação em bases de dados internacionais.

  7. NOTA EDITORIAL

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    Armando Cuesta Santos

    2009-02-01

    Full Text Available

    En este número de la revista hay un recorrido por un espectro  actualizado de las disciplinas fundamentales de la Ingeniería Industrial de esta contemporaneidad: la calidad, la dirección y la toma de decisiones consustancial, la gestión de procesos vinculados a la logística, la investigación de operaciones, y el factor humano asociado a la gestión de recursos humanos. Destaca la consideración de esas disciplinas y sus interacciones (reafianzando el carácter interdisciplinario de la Ingeniería Industrial en el ámbito de organizaciones laborales dedicadas a los servicios. Hospitales y empresas de servicios asociados a los mismos, así como una empresa de telecomunicaciones, devienen aquí objeto de estudio destacado.

  8. Notas Editoriales

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    Leyva José Pablo

    1958-09-01

    Full Text Available I. Es justa que el Instituto de Ciencias Naturales de la Universidad Nacional se asocie al centenario del nacimiento del naturalista colombiano don Joaquín Antonio Uribe, que su tierra natal va a celebrar con solemnidad en el presente año. / II. Se completan en este año dos centurias de la publicación del Systema Naturae de Carl von Linne, en su decima edición. Aprovecha esa oportunidad CALDASIA para reproducir en sus paginas el magnifico retrato del sabio sueco, que se conserva en el Museo Histórico de Bogotá.

  9. NOTA EDITORIAL

    Directory of Open Access Journals (Sweden)

    Armando Cuesta Santos

    2009-09-01

    Full Text Available

    En este número de la revista Ingeniería Industrial: gestión del conocimiento respecto a procesos de dirección, comunicación organizacional, estrategias logísticas, redes de cooperación en el desarrollo local, planificación de la calidad, mejora de procesos, modelado de procesos (mejoramiento de flujos de trabajo y del conocimiento, gestión de calidad junto a valores culturales, y desarrollo del potencial humano con enfoque de competencias, constituyen los temas centrales del conjunto de los diez (10 artículos científicos que se presentan.

  10. Notas varias

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    Avances de Enfermería Revista

    1996-06-01

    Full Text Available

    Egresados Facultad de Enfermería Universidad Nacional II semestre 1995 I semestre 1996 / Libros


  11. Using Abdominal CT Data for Visceral Fat Evaluation

    Directory of Open Access Journals (Sweden)

    M Pop

    2013-10-01

    Full Text Available Background: Quantitative assessment of body fat is important for the diagnosis and treatment of diseases related to obesity, Computed tomography (CT becoming the standard procedure for measuring the abdominal fat distribution. Material and method: The retrospective study included 111 inpatients, who underwent routine abdominal CT exams in the Radiology Laboratory of SCJU Tg.Mures (2013. MPR MDCT (SOMATOM AS 64 data was processed using a custom written MATLAB R2009b software, ImageJ being used for tracing of the visceral fat area (VFA. Patient data (including blood glucose, cholesterol and triglycerides were analyzed using MO Excel and GraphPad Inprism5. Results: Visceral Fat percentage varied in population from 14.59-68.69 (SD = 11.83 with significant difference between sexes (male vs. female, 46.98 vs. 31.62, p 220 mg% and triglycerides >150 mg% are significantly associated with the VF percent (p <0.05. Overall there is a weak correlation between the lab variables and the measured fat, the strongest one being between triglycerides and the VFA (r = +0.23 and between age and VFA percentage (certain samples. Conclusions: The technique used should decreases the human error in marking of the fat areas providing a better estimation of the VF/VF percentage. CT measured VF relates with certain lab tests. Further analysis, is required for a better use of CT in obesity related pathology diagnosis and treatment

  12. Adipocitos, obesidad visceral, inflamación y enfermedad cardiovascular

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    Fernando Manzur, MD.,FACC.

    2010-09-01

    Full Text Available La obesidad es un importante problema de salud a nivel mundial. Se considera el resultado de la combinación de factores genéticos, alimentación inadecuada y falta de actividad física regular. La ingestión de una dieta de alta densidad energética, es la principal causa de obesidad visceral o central, ya que el exceso de energía se almacena en los adipocitos, que aumentan en tamaño y en número, o ambos, en especial los viscerales, produciendo un incremento en la tasa de lipólisis, que a su vez, estimula la secreción de citoquinas por leucocitos, macrófagos y adipocitos, y conduce a estado proinflamatorio, resistencia a la insulina y disfunción endotelial. Esta última, favorecida por el proceso inflamatorio, puede ser el vínculo de unión entre la obesidad y la enfermedad cardiovascular. Así, la disfunción del tejido adiposo representa el mecanismo etiopatogénico en el desarrollo de enfermedad cardiovascular, iniciado por la obesidad visceral.

  13. Association of visceral adiposity with oesophageal and junctional adenocarcinomas.

    LENUS (Irish Health Repository)

    Beddy, P

    2012-02-01

    BACKGROUND: Obesity is associated with an increased incidence of oesophageal and oesophagogastric junction adenocarcinoma, in particular Siewert types I and II. This study compared abdominal fat composition in patients with oesophageal\\/junctional adenocarcinoma with that in patients with oesophageal squamous cell carcinoma and gastric adenocarcinoma, and in controls. METHOD: In total, 194 patients (110 with oesophageal\\/junctional adenocarcinoma, 38 with gastric adenocarcinoma and 46 with oesophageal squamous cell carcinoma) and 90 matched control subjects were recruited. The abdominal fat area was assessed using computed tomography (CT), and the total fat area (TFA), visceral fat area (VFA) and subcutaneous fat area (SFA) were calculated. RESULTS: Patients with oesophageal\\/junctional adenocarcinoma had significantly higher TFA and VFA values compared with controls (both P < 0.001), patients with gastric adenocarcinoma (P = 0.013 and P = 0.006 respectively) and patients with oesophageal squamous cell carcinoma (both P < 0.001). For junctional tumours, the highest TFA and VFA values were seen in patients with Siewert type I tumours (respectively P = 0.041 and P = 0.033 versus type III; P = 0.332 and P = 0.152 versus type II). CONCLUSION: Patients with oesophageal\\/junctional adenocarcinoma, in particular oesophageal and Siewert type I junctional tumours, have greater CT-defined visceral adiposity than patients with gastric adenocarcinoma or oesophageal squamous cell carcinoma, or controls.

  14. The economic impact of visceral leishmaniasis on households in Bangladesh.

    Science.gov (United States)

    Anoopa Sharma, D; Bern, Caryn; Varghese, Beena; Chowdhury, Rajib; Haque, Rashidul; Ali, Mustakim; Amann, Josef; Ahluwalia, Indu B; Wagatsuma, Yukiko; Breiman, Robert F; Maguire, James H; McFarland, Deborah A

    2006-05-01

    To explore current patterns of diagnosis and treatment, quantify household economic impact and identify household strategies to cover the costs of visceral leishmaniasis (VL) care in rural Bangladesh. Structured interviews with 113 VL patients from 87 households documenting all provider visits and expenditures for health care for VL, and the ways in which the expenditures were covered. Patients paid a median of 7 visits to six different providers before beginning VL treatment. All visited the subdistrict government hospital at least once. While health care, including antileishmanial drug therapy, is officially available free of charge at government facilities, 79% of patients reported making informal payments for provider access, diagnostics and drug administration; only 14% of patients received their full drug course from this source. For the 58% of patients who purchased the full treatment course, drug cost constituted 34% of direct expenditure. Median direct expenditure for one VL patient was US$87 and median income lost was $40; median total expenditure was 1.2 times annual per capita income of our study population. Households employed multiple coping strategies to cover expenditures, most commonly sale or rental of assets (62%) and taking out loans (64%). Visceral leishmaniasis treatment causes a major economic burden in affected families. Control strategies for VL should facilitate timely, affordable diagnosis and treatment of patients to decrease the infection reservoir and to alleviate the economic burden of VL on households.

  15. Transient receptor potential cation channels in visceral sensory pathways.

    Science.gov (United States)

    Blackshaw, L Ashley

    2014-05-01

    The extensive literature on this subject is in direct contrast to the limited range of clinical uses for ligands of the transient receptor potential cation channels (TRPs) in diseases of the viscera. TRPV1 is the most spectacular example of this imbalance, as it is in other systems, but it is nonetheless the only TRP target that is currently targeted clinically in bladder sensory dysfunction. It is not clear why this discrepancy exists, but a likely answer is in the promiscuity of TRPs as sensors and transducers for environmental mechanical and chemical stimuli. This review first describes the different sensory pathways from the viscera, and on which nociceptive and non-nociceptive neurones within these pathways TRPs are expressed. They not only fulfil roles as both mechano- and chemo-sensors on visceral afferents, but also form an effector mechanism for cell activation after activation of GPCR and cytokine receptors. Their role may be markedly changed in diseased states, including chronic pain and inflammation. Pain presents the most obvious potential for further development of therapeutic interventions targeted at TRPs, but forms of inflammation are emerging as likely to benefit also. However, despite much basic research, we are still at the beginning of exploring such potential in visceral sensory pathways. © 2014 The British Pharmacological Society.

  16. Geometric and functional architecture of visceral sensory microcircuitry.

    Science.gov (United States)

    Negishi, Yoshikatsu; Kawai, Yoshinori

    2011-03-01

    Is microcircuit wiring designed deterministically or probabilistically? Does geometric architecture predict functional dynamics of a given neuronal microcircuit? These questions were addressed in the visceral sensory microcircuit of the caudal nucleus of the tractus solitarius (NTS), which is generally thought to be homogeneous rather than laminar in cytoarchitecture. Using in situ hybridization histochemistry and whole-cell patch clamp recordings followed by neuronal reconstruction with biocytin filling, anatomical and functional organization of NTS microcircuitry was quantified to determine associative relationships. Morphologic and chemical features of NTS neurons displayed different patterns of process arborization and sub-nuclear localization according to neuronal types: smaller cells featured presynaptic local axons and GABAergic cells were aggregated specifically within the ventral NTS. The results suggested both a laminar organization and a spatial heterogeneity of NTS microcircuit connectivity. Geometric analysis of pre- and postsynaptic axodendritic arbor overlap of reconstructed neurons (according to parent somal distance) confirmed a heterogeneity of microcircuit connectivity that could underlie differential functional dynamics along the dorsoventral axis. Functional dynamics in terms of spontaneous and evoked postsynaptic current patterns behaved in a strongly location-specific manner according to the geometric dimension, suggesting a spatial laminar segregation of neuronal populations: a dorsal group of high excitation and a ventral group of balanced excitation and inhibition. Recurrent polysynaptic activity was also noted in a subpopulation of the ventral group. Such geometric and functional laminar organization seems to provide the NTS microcircuit with both reverberation capability and a differentiated projection system for appropriate computation of visceral sensory information.

  17. Epidemiology of visceral leishmaniasis in Algeria: an update.

    Science.gov (United States)

    Adel, Amel; Boughoufalah, Amel; Saegerman, Claude; De Deken, Redgi; Bouchene, Zahida; Soukehal, Abdelkrim; Berkvens, Dirk; Boelaert, Marleen

    2014-01-01

    Visceral leishmaniasis (VL), a zoonotic disease caused by Leishmania infantum, is endemic in Algeria. This report describes a retrospective epidemiological study conducted on human VL to document the epidemiological profile at national level. All human VL cases notified by the National Institute of Public Health between 1998 and 2008 were investigated. In parallel all VL cases admitted to the university hospitals of Algiers were surveyed to estimate the underreporting ratio. Fifteen hundred and sixty-two human VL cases were reported in Algeria between 1998-2008 with an average annual reported incidence rate of 0.45 cases per 100,000 inhabitants, of which 81.42% were in the age range of 0-4 years. Cases were detected year-round, with a peak notification in May and June. One hundred and seventy patients were admitted to the university hospitals in Algiers in the same period, of which less than one in ten had been officially notified. Splenomegaly, fever, pallor and pancytopenia were the main clinical and laboratory features. Meglumine antimoniate was the first-line therapy for paediatric VL whereas the conventional amphotericin B was used for adult patients. Visceral leishmaniasis in Algeria shows the epidemiological profile of a paediatric disease with a decrease of the annual reported incidence rate. However, vigilance is required because of huge underreporting and an apparent propagation towards the south.

  18. Nota a «Paradosso 2012/2. Forme della vita e statuti del vivente», AA.VV. (25/07/2013. Due parole su evoluzione sincronica, diacronica, flussi di energia e big crunch epistemologico

    Directory of Open Access Journals (Sweden)

    Franco Rebuffo

    2014-03-01

    Full Text Available Nota di Franco Rebuffo del 25/07/13 in risposta alla nota di Giorgio Forti del 09/07/2013 riguardante il numero di «Paradosso 2012/2. Forme della vita e statuti del vivente» a cura di Florinda Cambria.

  19. A systematic study on the utility of CHX-A''-DTPA-NCS and NOTA-NCS as bifunctional chelators for177Lu radiopharmaceuticals.

    Science.gov (United States)

    Pandey, Usha; Gamre, Naresh; Lohar, Sharad Pandurang; Dash, Ashutosh

    2017-09-01

    This paper describes the evaluation of [(R)-2-Amino-3-(4-isothiocyanatophenyl)propyl]-trans-(S,S)-cyclohexane-1,2-diamine-pentaacetic acid (CHX-A''-DTPA-NCS) and 2-S-(4-isothiocyanatobenzyl)-1,4,7-triazacyclononane-1,4,7-triacetic acid (NOTA-NCS) as bifunctional chelators for 177 Lu. While 177 Lu-CHX-A''-DTPA-NCS could be obtained in high yields at equimolar ratios of lutetium to CHX-A''-DTPA-NCS, >95% yield of 177 Lu-NOTA-NCS could be achieved at 1:2M ratio of lutetium to NOTA-NCS. Trace metals reduced the yields of 177 Lu-NOTA-NCS significantly as compared to 177 Lu-CHX-A''-DTPA-NCS. In vitro stability of 177 Lu-CHX-A''-DTPA-NCS was also superior to 177 Lu-NOTA-NCS. It could be concluded from this study that among the two chelators evaluated, CHX-A''-DTPA-NCS is more appropriate for preparation of 177 Lu radiopharmaceuticals. Copyright © 2017 Elsevier Ltd. All rights reserved.

  20. THE TECHNOLOGY OF GROWING Rosa canina L. AND Pelargonium zonale L. SEEDLINGS IN TISSUE CULTURE AND THEIR ADAPTATION IN DIFFERENT SUBSTRATES

    Directory of Open Access Journals (Sweden)

    Monika Tkalec

    2017-01-01

    Full Text Available The aim of this study was to establish the possibility of growing Rosa canina L. and Pelargonium zonale L. in tissue culture and suitability of alternative components and their mixtures as a substrate for adaptation of in vitro seedlings of these floral species. In this research, four alternative components (cocoa shell (A, willow bark (B, spent mushroom substrate (C, sawdust (D and six of their mixtures were analysed. Klasman Potgrond P (for wild rose and Balkon – blumenerde (for pelargonium were analysed as a control substrate. Statistical analysis has revealed significant differences between all the treatments (substrates and their analysed properties. An average percentage of adapted seedlings in all of the substrates was 54.68% for wild rose, and 99.68% for pelargonium. The largest values of morphological properties for the wild rose were determined on an M2 (30% A + 20% B + 40% C + 10% D substrate mixture, where the greatest number of shoots, leaf number, fresh overground mass, total fresh mass of seedling and the ratio of fresh and dry overground mass and root were recorded. Chemical composition of wild rose seedlings varied among the treatments, but the highest content of macro and micro elements was determined in the seedlings grown on sawdust (D and control substrate (E. The largest values of almost all morphological properties of pelargonium were also recorded on substrate mixtures M4 (18% A + 22% B + 40% C + 20% D and M6 (18% A + 42% B + 20% C + 20% D. Furthermore, while the largest morphological properties of pelargonium seedlings were recorded in the mixtures, the highest content of macro and micro elements determined in the seedlings was on those grown on alternative components, especially in the seedlings grown on a willow bark substrate.

  1. Reatividade vascular da artéria mamária interna: estudos farmacológicos comparativos entre artérias caninas direita e esquerda

    Directory of Open Access Journals (Sweden)

    EVORA Paulo Roberto B.

    1999-01-01

    Full Text Available Para estudos comparativos da reatividade vascular entre artéria mamária interna (AMI canina direita e esquerda, realizaram-se experimentos "in vitro" utilizando-se banhos orgânicos ("organ chambers" e ensaios biológicos: 1 os produtos plaquetários ADP e 5-HT induziram, respectivamente, vasodilatação dependente e independente do endotélio; 2 os autacóides, bradicinina e histamina, também induziram vasodilatação, respectivamente, dependente e independente do endotélio; 3 o A23187, vasodilatador independente de receptor, induziu relaxamentos dependentes do endotélio; 4 dopamina, dobutamina, papaverina e a poli-L-arginina induziram vasodilatações independentes do endotélio; 5 a NOR induziu intensa vasoconstrição comparável à causada pelo KCI e pela endotelina; 6 em 83% de 24 ensaios, a liberação basal de NO foi maior na AMI esquerda, em comparação com a AMI direita; 7 ensaios biológicos de AMIs demonstraram a importância da PGI2 nas condições de hipóxia, uma vez que a indometacina aboliu vasodilatação adicional em resposta à hipóxia em condições de vasodilatação induzida pela liberação basal de NO; 8 não ocorreram diferenças significantes de resposta, comparando-se estudos realizados em AMIs direita e esquerda.

  2. Switching harmful visceral fat to beneficial energy combustion improves metabolic dysfunctions.

    Science.gov (United States)

    Yang, Xiaoyan; Sui, Wenhai; Zhang, Meng; Dong, Mei; Lim, Sharon; Seki, Takahiro; Guo, Ziheng; Fischer, Carina; Lu, Huixia; Zhang, Cheng; Yang, Jianmin; Zhang, Meng; Wang, Yangang; Cao, Caixia; Gao, Yanyan; Zhao, Xingguo; Sun, Meili; Sun, Yuping; Zhuang, Rujie; Samani, Nilesh J; Zhang, Yun; Cao, Yihai

    2017-02-23

    Visceral fat is considered the genuine and harmful white adipose tissue (WAT) that is associated to development of metabolic disorders, cardiovascular disease, and cancer. Here, we present a new concept to turn the harmful visceral fat into a beneficial energy consumption depot, which is beneficial for improvement of metabolic dysfunctions in obese mice. We show that low temperature-dependent browning of visceral fat caused decreased adipose weight, total body weight, and body mass index, despite increased food intake. In high-fat diet-fed mice, low temperature exposure improved browning of visceral fat, global metabolism via nonshivering thermogenesis, insulin sensitivity, and hepatic steatosis. Genome-wide expression profiling showed upregulation of WAT browning-related genes including Cidea and Dio2. Conversely, Prdm16 was unchanged in healthy mice or was downregulated in obese mice. Surgical removal of visceral fat and genetic knockdown of UCP1 in epididymal fat largely ablated low temperature-increased global thermogenesis and resulted in the death of most mice. Thus, browning of visceral fat may be a compensatory heating mechanism that could provide a novel therapeutic strategy for treating visceral fat-associated obesity and diabetes.

  3. So as we worry we weigh: Visible burrow system stress and visceral adiposity.

    Science.gov (United States)

    Foster, Michelle T

    2017-09-01

    The visible borrow system (VBS) simulates a natural rodent habitat that supports genuine stress provoking social interactions. This model allows investigation of behavioral, neural and endocrine alterations caused by chronic stress. The Sakai lab further used this model to investigate metabolic outcomes of stress in relation to dominance hierarchies formed within the VBS. Communal social conflict occurs among all VBS rats, but only the SUB rats succumb to the redistribution of lipids in the visceral cavity and consequent metabolic dysregulation, such as hyper-insulinemia. These increases in visceral adipose tissue occur after two cycles of VBS stress and recovery bouts and are associated with decreases in subcutaneous adipose tissue. Traditionally, distribution shift in lipid deposition is predominately thought to occur by characteristics specific to the visceral depot, but evidence supports that decreased subcutaneous adipose tissue deposition may be linked to enhanced visceral adipose expansion. This review will discuss VBS stress and redirection of adipose tissue in SUB rats. There will be specific focus on the enhanced adipogenic capacity of visceral adipose tissue as driven by glucocorticoid receptor density, 11-hydroxysteroid dehydrogenase type 1 (11-HSD1) and lipoprotein lipase (LPL). Additionally, the proposed contribution of decreased subcutaneous adipose expansion via stress-induced inhibition of lipid uptake, storage and cellularity will be discussed. Overall, this review will summarize how stress-induced visceral obesity may result from a combination of maladaptive responses within the visceral and subcutaneous depot. Copyright © 2017 Elsevier Inc. All rights reserved.

  4. Roles of prefrontal cortex and paraventricular thalamus in affective and mechanical components of visceral nociception.

    Science.gov (United States)

    Jurik, Angela; Auffenberg, Eva; Klein, Sabine; Deussing, Jan M; Schmid, Roland M; Wotjak, Carsten T; Thoeringer, Christoph K

    2015-12-01

    Visceral pain represents a major clinical challenge in the management of many gastrointestinal disorders, eg, pancreatitis. However, cerebral neurobiological mechanisms underlying visceral nociception are poorly understood. As a representative model of visceral nociception, we applied cerulein hyperstimulation in C57BL6 mice to induce acute pancreatitis and performed a behavioral test battery and c-Fos staining of brains. We observed a specific pain phenotype and a significant increase in c-Fos immunoreactivity in the paraventricular nucleus of the thalamus (PVT), the periaqueductal gray, and the medial prefrontal cortex (mPFC). Using neuronal tracing, we observed projections of the PVT to cortical layers of the mPFC with contacts to inhibitory GABAergic neurons. These inhibitory neurons showed more activation after cerulein treatment suggesting thalamocortical "feedforward inhibition" in visceral nociception. The activity of neurons in pancreatitis-related pain centers was pharmacogenetically modulated by designer receptors exclusively activated by designer drugs, selectively and cell type specifically expressed in target neurons using adeno-associated virus-mediated gene transfer. Pharmacogenetic inhibition of PVT but not periaqueductal gray neurons attenuated visceral pain and induced an activation of the descending inhibitory pain pathway. Activation of glutamatergic principle neurons in the mPFC, but not inhibitory neurons, also reversed visceral nociception. These data reveal novel insights into central pain processing that underlies visceral nociception and may trigger the development of novel, potent centrally acting analgesic drugs.

  5. Intra-Aortic Balloon Pump Malposition Reduces Visceral Artery Perfusion in an Acute Animal Model.

    Science.gov (United States)

    Vondran, Maximilian; Rastan, Ardawan J; Tillmann, Eugen; Seeburger, Jörg; Schröter, Thomas; Dhein, Stefan; Bakhtiary, Farhad; Mohr, Friedrich-Wilhelm

    2016-04-01

    Visceral artery perfusion can be potentially affected by intra-aortic balloon pump (IABP) catheters. We utilized an animal model to quantify the acute impact of a low balloon position on mesenteric artery perfusion. In six pigs (78 ± 7 kg), a 30-cc IABP was placed in the descending aorta in a transfemoral procedure. The celiac artery (CA) and the cranial mesenteric artery (CMA) were surgically dissected. Transit time blood flow was measured for (i) baseline, (ii) 1:1 augmentation with the balloon proximal to the visceral arteries, and (iii) 1:1 augmentation with the balloon covering the visceral arteries. Blood flow in the CMA and CA was reduced by 17 and 24%, respectively, when the balloon compromised visceral arteries compared with a position above the visceral arteries (flow in mL/min: CMA: (i) 1281 ± 512, (ii) 1389 ± 287, (iii) 1064 ± 276, P < 0.05 for 3 vs. 1 and 3 vs. 2; CA: (i) 885 ± 370, (ii) 819 ± 297, (iii) 673 ± 315; P < 0.05 for 3 vs. 1). The covering of visceral arteries by an IABP balloon causes a significant reduction of visceral artery perfusion; thus, the positioning of this device during implantation is critical for obtaining a satisfactory outcome. Copyright © 2015 International Center for Artificial Organs and Transplantation and Wiley Periodicals, Inc.

  6. Visceral obesity and psychosocial stress: a generalised control theory model

    Science.gov (United States)

    Wallace, Rodrick

    2016-07-01

    The linking of control theory and information theory via the Data Rate Theorem and its generalisations allows for construction of necessary conditions statistical models of body mass regulation in the context of interaction with a complex dynamic environment. By focusing on the stress-related induction of central obesity via failure of HPA axis regulation, we explore implications for strategies of prevention and treatment. It rapidly becomes evident that individual-centred biomedical reductionism is an inadequate paradigm. Without mitigation of HPA axis or related dysfunctions arising from social pathologies of power imbalance, economic insecurity, and so on, it is unlikely that permanent changes in visceral obesity for individuals can be maintained without constant therapeutic effort, an expensive - and likely unsustainable - public policy.

  7. Visceral leishmaniasis and HIV coinfection in the Mediterranean region.

    Directory of Open Access Journals (Sweden)

    Begoña Monge-Maillo

    2014-08-01

    Full Text Available Visceral leishmaniasis is hypoendemic in Mediterranean countries, where it is caused by the flagellate protozoan Leishmania infantum. VL cases in this area account for 5%-6% of the global burden. Cases of Leishmania/HIV coinfection have been reported in the Mediterranean region, mainly in France, Italy, Portugal, and Spain. Since highly active antiretroviral therapy was introduced in 1997, a marked decrease in the number of coinfected cases in this region has been reported. The development of new diagnostic methods to accurately identify level of parasitemia and the risk of relapse is one of the main challenges in improving the treatment of coinfected patients. Clinical trials in the Mediterranean region are needed to determine the most adequate therapeutic options for Leishmania/HIV patients as well as the indications and regimes for secondary prophylaxis. This article reviews the epidemiological, diagnostic, clinical, and therapeutic aspects of Leishmania/HIV coinfection in the Mediterranean region.

  8. The unwelcome trio: HIV plus cutaneous and visceral leishmaniasis.

    Science.gov (United States)

    Guarneri, C; Tchernev, G; Bevelacqua, V; Lotti, T; Nunnari, G

    2016-01-01

    Leishmania/Human Immunodeficiency Virus (HIV) coinfection has emerged as an extremely serious and increasingly frequent health problem in the last decades. Considering the insidious and not typical clinical picture in presence of immunosuppressive conditions, the increasing number of people travelling in endemic zones, the ability to survive, within both human and vector bodies, of the parasite, clinicians and dermatologists as the first line should be aware of these kind of "pathologic alliances," to avoid delayed diagnosis and treatment. In this setting, the occurrence of cutaneous lesions can, paradoxically, aid the physician in recognition and approaching the correct staging and management of the two (or three) diseases. Treatment of these unwelcome synergies is a challenge: apart from the recommended anti-retroviral protocols, different anti-leishmanial drugs have been widely used, according with the standard guidelines for visceral leishmaniasis (VL), with no successful treatment regimen still been established. © 2015 Wiley Periodicals, Inc.

  9. Electronic protocol for clinical data collection in chronic visceral ischemia

    Directory of Open Access Journals (Sweden)

    Adriana Buechner de Freitas Brandão

    2017-04-01

    Full Text Available Abstract Patients with vascular diseases present with a long medical history which makes for complex and confusing medical records. Electronic record systems have a large storage capacity and high information processing capabilities, and may therefore improve the quality and reliability of prospective clinical studies. In the present study, a theoretical framework for clinical data collection in chronic visceral ischemia was created containing 5706 items, organized into six major categories: medical history, physical examination, laboratory testing, diagnosis, treatment and outcome. The database was used to construct an electronic data collection protocol, which was uploaded into the Integrated Electronic Protocol System (Sistema Integrado de Protocolos Eletrônicos, SINPE© and then used to perform retrospective collection and analysis of clinical data from 10 patients using the SINPE© analysis module.

  10. Visceral Obesity is Associated with Poor Prognosis in Pancreatic Adenocarcinoma.

    Science.gov (United States)

    Kim, Bun; Chung, Moon Jae; Park, Se Woo; Park, Jeong Youp; Bang, Seungmin; Park, Seung Woo; Song, Si Young; Chung, Jae Bock

    2016-01-01

    An association between obesity and unfavorable outcomes for various types of malignancy has been established. Nevertheless, the impact of visceral obesity (VO) on outcomes in pancreatic cancer is still unknown and controversial. The aim of this study was to uncover an association between VO and pancreatic cancer outcomes. We retrospectively reviewed 499 patients with pancreatic cancer who were diagnosed and treated in Severance Hospital from January 2006 to December 2011. Compared to the low-VO group (n = 260), the high-VO group (n = 239) was mostly male (68.2% vs. 31.8%, P alcohol intake status (52.3% vs. 26.4%, P pancreatic cancer, VO at the time of diagnosis is associated with negative outcomes, such as shorter PFS and OS.

  11. [General principles of tumour biology in visceral surgery].

    Science.gov (United States)

    Emons, G; Ghadimi, M; Grade, M

    2015-02-01

    Within the last decade, there has been a tremendous progress in understanding the molecular basis of cancer. In particular, the development and the characteristic features of cancer cells are being increasingly understood. The understanding of these molecular characteristics is mandatory for the development of novel, targeted therapeutic strategies and their integration into clinical practice. In addition, tumour genetics play a critically important role for hereditary cancer syndromes, with respect to both diagnostics and clinical decision-making. The aim of this review is to highlight general principles of tumour genetics from a visceral surgeon's point of view, although a comprehensive summary of all aspects would be beyond the scope of this article due to the complexity of the topic. Georg Thieme Verlag KG Stuttgart · New York.

  12. Visceral and presumptive neural baylisascariasis in an orangutan (Pongo pygmaeus).

    Science.gov (United States)

    Hanley, Christopher S; Simmons, Heather A; Wallace, Roberta S; Clyde, Victoria L

    2006-12-01

    A 32.5-year-old female hybrid orangutan (Pongo pygmaeus) developed hind-limb stiffness that progressed to tetraparesis over 2 wk. Repeated diagnostic evaluations, including serial magnetic resonance imaging of the central nervous system, revealed nonspecific lesions involving both the deep white and gray matter with an intact blood-brain barrier. Multiple empirical treatments failed to produce improvement and the animal was humanely euthanized. Histology of a granuloma in the ileum contained a nematode parasite, most consistent with Baylisascaris procyonis. Additionally, neuropil vacuolization, rarefaction, astrocytic scarring, and an eosinophilic granuloma and lymphoeosinophilic perivascular cuffing in the brain were suggestive of nematode migration. These findings confirm the presence of visceral larval migrans and support the presence of neural larval migrans. This case report of Baylisascaris procyonis confirms the presentation for the first time in an ape and documents the difficulty in antemortem diagnosis of neural larval migrans.

  13. Visceral adipose tissue is associated with microstructural brain tissue damage.

    Science.gov (United States)

    Widya, Ralph L; Kroft, Lucia J M; Altmann-Schneider, Irmhild; van den Berg-Huysmans, Annette A; van der Bijl, Noortje; de Roos, Albert; Lamb, Hildo J; van Buchem, Mark A; Slagboom, P Eline; van Heemst, Diana; van der Grond, Jeroen

    2015-05-01

    Obesity has been associated with microstructural brain tissue damage. Different fat compartments demonstrate different metabolic and endocrine behaviors. The aim was to investigate the individual associations between abdominal visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT) and microstructural integrity in the brain. This study comprised 243 subjects aged 65.4 ± 6.7 years. The associations between abdominal VAT and SAT, assessed by CT, and magnetization transfer imaging markers of brain microstructure for gray and white matter were analyzed and adjusted for confounding factors. VAT was associated with normalized MTR peak height in gray (β -0.216) and white matter (β -0.240) (both P  0.05). Stepwise linear regression analysis showed that only VAT was associated with normalized MTR peak height in gray and white matter (both P VAT rather than SAT is associated with microstructural brain tissue damage in elderly individuals. © 2015 The Obesity Society.

  14. Early visceral pain predicts chronic pain after laparoscopic cholecystectomy

    DEFF Research Database (Denmark)

    Blichfeldt-Eckhardt, Morten Rune; Ording, Helle; Andersen, Claus

    2014-01-01

    Chronic pain after laparoscopic cholecystectomy is related to postoperative pain during the first postoperative week, but it is unknown which components of the early pain response is important. In this prospective study, 100 consecutive patients were examined preoperatively, 1week postoperatively...... during the first postoperative week. Nine patients developed chronic unexplained pain 12months postoperatively. In a multivariate analysis model, cumulated visceral pain during the first week and number of preoperative biliary pain attacks were identified as independent risk factors for unexplained...... chronic pain 12months postoperatively. There were no consistent signs of hypersensitivity in the referred pain area either pre- or postoperatively. There were no significant associations to any other variables examined. The risk of chronic pain after laparoscopic cholecystectomy is relatively low...

  15. Enzymatic degradation of kombu powder by visceral extract of shellfish

    Energy Technology Data Exchange (ETDEWEB)

    Onishi, Toshio; Suzuki, Mampei; Kikuchi, Ryo

    1986-03-01

    With an objective of energy-saving decomposition of polysaccharides of brown alage for producing monosaccharide or for utilizing as a fodder, Kombu powder was decomposed with visceral extract of shellfish. Relation between enzyme amount and decomposition rate was examined at pH 6 which was optimum for Kombu decomposition. Protein concentration and decomposition rate were proportional in the range of enzyme amount tested. When shellfish enzyme and Meicelase (commercial cellulose) were used in mixture, Kombu decomposition proceeded gradually except the initial several hours. In the test using the enzyme quantity and longer time, the production of reducing sugar kept increasing even after 24 hours if the enzyme quantity was ample enough, showing the possibility of attaining higher decomposition. On the other hand, rate of decomposition per unit protein decreased by the higher enzyme concentration. For Meicelase P, the maximum decomposition was 42 % when crystalline cellulose was used as a base material. (4 figs, 1 tab, 13 refs)

  16. Visceral fat accumulation is associated with colorectal cancer in postmenopausal women.

    Directory of Open Access Journals (Sweden)

    Jee-Yon Lee

    Full Text Available BACKGROUND: Obesity is a known risk factor for colorectal cancer (CRC, and emerging data suggest that this association is mediated by visceral fat rather than total body fat. However, there is a lack of studies evaluating the association between visceral fat area and the prevalence of CRC. METHODS: To investigate the relationship between visceral adiposity and prevalence of CRC, data of 497 women diagnosed with CRC and 318 apparently healthy women were analysed and data of well-balanced 191 pairs of women with CRC and healthy women matched based on propensity scores were additionally analysed. Diagnosis of CRC was confirmed by colonoscopy and histology. Metabolic parameters were assessed, along with body composition, using computed tomography. RESULTS: The median visceral fat area was significantly higher in the CRC group compared with the control group before and after matching. The prevalence of CRC increased significantly with increasing visceral fat tertiles after matching (p for trend <0.01. A multivariate analysis showed that mean visceral fat area of individuals in the 67th percentile or greater group was associated with an increased prevalence of CRC (adjusted odds ratio: 1.80; 95% confidence interval: 1.12-2.91 before matching and adjusted odds ratio: 2.96; 95% confidence interval: 1.38-6.33 compared with that of individuals in the 33th percentile or lower group. CONCLUSION: Thus, we conclude that visceral fat area is positively associated with the prevalence of CRC. Although we could not determine the causality, visceral adiposity may be associated with the risk of CRC. Further prospective studies are required to determine the benefits of controlling visceral obesity for reducing CRC risk.

  17. Measurement of visceral fat: should we include retroperitoneal fat?

    Directory of Open Access Journals (Sweden)

    Chi-Sheng Hung

    Full Text Available OBJECTIVE: Whether retroperitoneal fat should be included in the measurement of visceral fat remains controversial. We compared the relationships of fat areas in peritoneal, retroperitoneal, and subcutaneous compartments to metabolic syndrome, adipokines, and incident hypertension and diabetes. METHODS: We enrolled 432 adult participants (153 men and 279 women in a community-based cohort study. Computed tomography at the umbilicus level was used to measure the fat areas. RESULTS: Retroperitoneal fat correlated significantly with metabolic syndrome (adjusted odds ratio (OR, 5.651, p<0.05 and the number of metabolic abnormalities (p<0.05. Retroperitoneal fat area was significantly associated with blood pressure, plasma glycemic indices, lipid profile, C-reactive protein, adiponectin (r =  -0.244, P<0.05, and leptin (r = 0.323, p<0.05, but not plasma renin or aldosterone concentrations. During the 2.94 ± 0.84 years of follow-up, 32 participants developed incident hypertension. Retroperitoneal fat area (hazard ration (HR 1.62, p = 0.003 and peritoneal fat area (HR 1.62, p = 0.009, but not subcutaneous fat area (p = 0.14 were associated with incident hypertension. Neither retroperitoneal fat area, peritoneal fat area, nor subcutaneous fat areas was associated with incident diabetes after adjustment. CONCLUSIONS: Retroperitoneal fat is similar to peritoneal fat, but differs from subcutaneous fat, in terms of its relationship with metabolic syndrome and incident hypertension. Retroperitoneal fat area should be included in the measurement of visceral fat for cardio-metabolic studies in human.

  18. Therapeutic effect of ursolic acid in experimental visceral leishmaniasis

    Directory of Open Access Journals (Sweden)

    Jéssica A. Jesus

    2017-04-01

    Full Text Available Leishmaniasis is an important neglected tropical disease, affecting more than 12 million people worldwide. The available treatments are not well tolerated and present diverse side effects in patients, justifying the search for new therapeutic compounds. In the present study, the therapeutic potential and toxicity of ursolic acid (UA, isolated from the leaves of Baccharis uncinella C. DC. (Asteraceae, were evaluated in experimental visceral leishmaniasis. To evaluate the therapeutic potential of UA, hamsters infected with L. (L. infantum were treated daily during 15 days with 1.0 or 2.0 mg UA/kg body weight, or with 5.0 mg amphotericin B/kg body weight by intraperitoneal route. Fifteen days after the last dose, the parasitism of the spleen and liver was stimated and the main histopathological alterations were recorded. The proliferation of splenic mononuclear cells was evaluated and IFN-γ, IL-4, and IL-10 gene expressions were analyzed in spleen fragments. The toxicity of UA and amphotericin B were evaluated in healthy golden hamsters by histological analysis and biochemical parameters. Animals treated with UA had less parasites in the spleen and liver when compared with the infected control group, and they also showed preservation of white and red pulps, which correlate with a high rate of proliferation of splenic mononuclear cells, IFN-γ mRNA and iNOS production. Moreover, animals treated with UA did not present alterations in the levels of AST, ALT, creatinine and urea. Taken together, these findings indicate that UA is an interesting natural compound that should be considered for the development of prototype drugs against visceral leishmaniasis.

  19. Splenic state in surviving patients with visceral heterotaxy.

    Science.gov (United States)

    Nagel, Bert H P; Williams, Helen; Stewart, Louise; Paul, Joanne; Stümper, Oliver

    2005-10-01

    To identify patients with visceral heterotaxy who are at risk from fulminant sepsis. We studied 38 patients, 37 having undergone abdominal ultrasound, all 38 having examination of blood films to establish presence of Howell-Jolly bodies, and all 38 documented to have had pneumococcal vaccination and prophylaxis with penicillin. We checked whether the parents were aware of the splenic state of their child, and when possible, we compared current results of blood films with those obtained postnatally. Two of the 17 patients with multiple spleens, all 11 without a detectable spleen, and 1 of 9 patients with a normal spleen, showed Howell-Jolly bodies in their blood films. In 5 of 23 patients with serial blood films, Howell-Jolly bodies had not been seen postnatally, but could now be detected in current blood films. Of these patients, 2 had multiple spleens, 1 did not have a spleen, and 1 had a solitary spleen of normal size. In the other patient, ultrasound could not be performed. Only one of these patients was receiving penicillin prophylactically, and had received pneumococcal vaccination. Of the 15 patients in whom Howell-Jolly bodies were present in the blood, only 8 parents knew about the potential risk for infection. Another 7 parents were sure that their child was taking penicillin regularly, and had received pneumococcal vaccination. Howell-Jolly bodies can be found in the blood of patients with visceral heterotaxy independent of the anatomical state of the spleen. As Howell-Jolly bodies can be encountered in the blood of such patients with increasing age, those with multiple and solitary spleens should be monitored regularly to identify those at risk. Parental knowledge of the splenic state, and compliance for prophylaxis using penicillin, and pneumococcal vaccination, were unsatisfactory in our cohort.

  20. Lutzomyia longipalpis (Diptera, Psychodidae, Phlebotominae) and urbanization of visceral leishmaniasis in Brazil.

    Science.gov (United States)

    Rangel, Elizabeth F; Vilela, Maurício L

    2008-12-01

    The article discusses habits related to the vectorial competence of Lutzomyia longipalpis, along with evidence confirming the importance of this sand fly species in the epidemiological chain of visceral leishmaniasis in Brazil. A new epidemiological profile for visceral leishmaniasis is also postulated, associated with domestic environments and the role of Lu. longipalpis in this process, its sylvatic origin, and its capacity to adapt to a wide range of habitats. Another sand fly species, Lu. cruzi, is mentioned as a vector of visceral leishmaniasis in some municipalities in Central Brazil, based on studies in endemic areas of the country.

  1. Notas sobre linguagem, texto e pesquisa histórica em educação

    Directory of Open Access Journals (Sweden)

    Maria do Rosário Longo Mortatti

    2012-06-01

    Full Text Available  Com o objetivo de contribuir para a discussão acerca dos problemas e possibilidades da pesquisa histórica em educação, apresentam-se nestas notas, com base na concepção interacionista de linguagem, reflexões problematizadoras sobre as relações entre as atividades de leitura e produção de textos e os processos: de recuperação, reunião, seleção e análise de fontes documentais escritas; de produção do texto final (monografia, dissertação ou tese em que se materializa discursivamente o objeto de investigação; e de constituição do sujeito desse discurso.Palavras-chave: linguagem, texto, pesquisa histórica em educação.   Abstract In order to contribute for the discussion on problems and possibilities of the historical research in education, it is shown in this text, based upon a linguistic interactional conception, reflexions about relations among the activities of reading and writing texts and the process of: recovering, reuniting, selecting and analyzing documental sources; producing the final text; and constituting the discours subject. Keywords: language, text, historical research in education. 

  2. Funkeiros, timbaleiros e pagodeiros: notas sobre juventude e música negra na cidade de Salvador

    Directory of Open Access Journals (Sweden)

    Ari Lima

    Full Text Available Neste artigo, pretendo discutir como, num contexto racializado da associação entre juventude negra e música, surgem experiências que se desenvolvem como marcas identitárias, crítica social e ratificação de hierarquias raciais, de classe e gênero. Para tanto, trabalho com as idéias de juventude e geração como dado biológico, tanto quanto social e histórico, e com a idéia de música como sentido compartilhado. Além disso, rediscuto minhas notas etnográficas e de outros autores que refletiram sobre a relação entre música e juventude negra, cujos jovens observados, em contextos diversificados, são basicamente suburbanos, pobres e "negro-mestiços" com baixo grau de escolaridade, alguns com baixa capacidade de consumo, outros consumidores ansiosos. Concluo apontando para a importância da música como instrumento configurador de uma experiência juvenil e negra afro-diaspórica, mas também como instrumento repositor de antigas dessemelhanças que não estão nos genes.

  3. Inmigración y asociacionismo: Notas de viaje Immigration and Associationism

    Directory of Open Access Journals (Sweden)

    William Fredy Pérez Toro

    2009-07-01

    Full Text Available A partir de la información obtenida mediante observación directa y entrevistas, y en el marco del proyecto de investigación sobre las organizaciones de inmigrantes colombianos en España, el artículo ofrece una reflexión derivada de las notas de un "diario de campo" en el que convergen los lugares, las prácticas, las percepciones y las formas de organización de los inmigrantes colombianos en Cataluña, examinados a partir de los enfoques del tema migratorio y la acción colectiva.From the information obtained through direct observation and interviews, and under the research project on Colombian immigrant organizations in Spain, the article offers a reflection derived from the notes of a "field journal" in which converge places, practices, perceptions and forms of organization of Colombian immigrants in Catalonia, considered from the issue of migration approaches and collective action.

  4. In vitro and in vivo evaluation of a (18F-labeled high affinity NOTA conjugated bombesin antagonist as a PET ligand for GRPR-targeted tumor imaging.

    Directory of Open Access Journals (Sweden)

    Zohreh Varasteh

    Full Text Available Expression of the gastrin-releasing peptide receptor (GRPR in prostate cancer suggests that this receptor can be used as a potential molecular target to visualize and treat these tumors. We have previously investigated an antagonist analog of bombesin (D-Phe-Gln-Trp-Ala-Val-Gly-His-Sta-Leu-NH2, RM26 conjugated to 1,4,7-triazacyclononane-N,N',N''-triacetic acid (NOTA via a diethylene glycol (PEG2 spacer (NOTA-P2-RM26 labeled with (68Ga and (111In. We found that this conjugate has favorable properties for in vivo imaging of GRPR-expression. The focus of this study was to develop a (18F-labelled PET agent to visualize GRPR. NOTA-P2-RM26 was labeled with (18F using aluminum-fluoride chelation. Stability, in vitro binding specificity and cellular processing tests were performed. The inhibition efficiency (IC50 of the [(natF]AlF-NOTA-P2-RM26 was compared to that of the (natGa-loaded peptide using (125I-Tyr(4-BBN as the displacement radioligand. The pharmacokinetics and in vivo binding specificity of the compound were studied. NOTA-P2-RM26 was labeled with (18F within 1 h (60-65% decay corrected radiochemical yield, 55 GBq/µmol. The radiopeptide was stable in murine serum and showed high specific binding to PC-3 cells. [(natF]AlF-NOTA-P2-RM26 showed a low nanomolar inhibition efficiency (IC50=4.4±0.8 nM. The internalization rate of the tracer was low. Less than 14% of the cell-bound radioactivity was internalized after 4 h. The biodistribution of [(18F]AlF-NOTA-P2-RM26 demonstrated rapid blood clearance, low liver uptake and low kidney retention. The tumor uptake at 3 h p.i. was 5.5±0.7 %ID/g, and the tumor-to-blood, -muscle and -bone ratios were 87±42, 159±47, 38±16, respectively. The uptake in tumors, pancreas and other GRPR-expressing organs was significantly reduced when excess amount of non-labeled peptide was co-injected. The low uptake in bone suggests a high in vivo stability of the Al-F bond. High contrast PET image was obtained 3 h p

  5. Inverse association between visceral obesity and lymph node metastasis in gastric cancer.

    Science.gov (United States)

    Park, Se Woo; Lee, Hang Lak; Ju, Yong Won; Jun, Dae Won; Lee, Oh Young; Han, Dong Soo; Yoon, Byung Chul; Choi, Ho Soon; Hahm, Joon Soo

    2015-02-01

    The relationship between fat distribution and lymph node metastasis has not been well studied. The goal of this study was to determine the impact of visceral obesity on lymph node metastasis in gastric cancer. Metastatic lymph node ratio (MLR) was defined as the number of involved nodes by tumor divided by the total number of resected lymph nodes. Visceral (VFA) and subcutaneous fat areas (SFA) were determined by measuring abdominal fat volume distribution via CT scan, and visceral obesity was defined as a VFA to total fat area ratio (V/T) >0.29. With lymph node metastasis as a dependent variable, the following factors were significant in multivariate analysis among 495 patients: pathologic T stage (P obesity defined by higher visceral to total fat area ratio was significantly associated with decreased MLR.

  6. Acute large bowel pseudo-obstruction due to atrophic visceral myopathy: A case report

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    Sean M. Wrenn

    2017-01-01

    Conclusions: Atrophic visceral neuropathy is a rare cause of intestinal pseudo-obstruction. While often presenting with chronic obstruction in younger populations, we present a rare late-onset acute presentation that may have been secondary to underlying hypothyroidism.

  7. Clinical severity of visceral leishmaniasis is associated with changes in immunoglobulin g fc N-glycosylation.

    NARCIS (Netherlands)

    Gardinassi, L.G.; Dotz, V.; Hipgrave Ederveen, A.; de Almeida, R.P.; Nery Costa, C.H.; Costa, D.L.; de Jesus, A.R.; Mayboroda, O.A.; Garcia, G.R.; Wuhrer, M.; de Miranda Santos, I.K.

    2014-01-01

    Visceral leishmaniasis (VL) has a high fatality rate if not treated; nevertheless, the majority of human infections with the causative agent, Leishmania infantum chagasi, are asymptomatic. Although VL patients often present with increased levels of serum immunoglobulins, the contribution of

  8. The Gut and Energy Balance: Visceral Allies in the Obesity Wars

    National Research Council Canada - National Science Library

    Michael K. Badman; Jeffrey S. Flier

    2005-01-01

    .... The gut, the pancreatic islets of Langerhans, elements in the portal vasculature, and even visceral adipose tissue communicate with the controllers of energy balance in the brain by means of neural...

  9. Rectal Visceral Sensitivity in Women with Irritable Bowel Syndrome without Psychiatric Comorbidity Compared with Healthy Volunteers

    Directory of Open Access Journals (Sweden)

    Signe Spetalen

    2009-01-01

    Conclusions. Non-constipated IBS patients without psychiatric disorders had increased visceral sensitivity regarding volume thresholds but normal pressure thresholds. Our study suggests that the lowered volume threshold was due to increased rectal tone.

  10. Liposomal amphotericin B versus pentavalent antimony salts for visceral Leishmania in children

    National Research Council Canada - National Science Library

    Apa, Hurşit; Devrim, İlker; Bayram, Nuri; Deveci, Reyhan; Demir-Özek, Gülcihan; Cartı, Özgür Umaç

    2013-01-01

    The aim of this study was to investigate the efficacy of a 21-day schedule of liposomal amphotericin B compared to pentavalent antimony salts in the treatment of patients during a first episode of visceral leishmaniasis...

  11. Failure of miltefosine in visceral leishmaniasis is associated with low drug exposure

    NARCIS (Netherlands)

    Dorlo, Thomas P C; Rijal, Suman; Ostyn, Bart; de Vries, Peter J; Singh, Rupa; Bhattarai, Narayan; Uranw, Surendra; Dujardin, Jean-Claude; Boelaert, Marleen; Beijnen, Jos H; Huitema, Alwin D R

    2014-01-01

    BACKGROUND: Recent reports indicated high miltefosine treatment failure rates for visceral leishmaniasis (VL) on the Indian subcontinent. To further explore the pharmacological factors associated with these treatment failures, a population pharmacokinetic-pharmacodynamic study was performed to

  12. Mucosal relapse of visceral leishmaniasis in a child treated with anti-TNFα

    Directory of Open Access Journals (Sweden)

    E. Jeziorski

    2015-04-01

    Full Text Available Visceral leishmaniasis is an enzootic parasitosis present across the Mediterranean Basin. Some consider it an opportunistic parasite. We report the case of a girl treated with anti-tumour necrosis factor alpha (anti-TNFα for juvenile idiopathic arthritis who had previously presented with visceral leishmaniasis. Two and a half years later, she presented a tumour-like mass in the nasal mucous membrane caused by Leishmania parasites. Leishmania infantum is classically responsible for visceral leishmaniasis, but pure mucocutaneous leishmaniasis has also been described. To our knowledge, this is the first observation of a recurrence of visceral leishmaniasis in the mucocutaneous form. The occurrence of atypical forms and presentations in those on anti-TNF therapy should be considered.

  13. Nota [N] Roja la vibrante historia de un género y una nueva manera de informar

    Directory of Open Access Journals (Sweden)

    Blasco Fernando Checa Montúfar

    2010-06-01

    Full Text Available Presenta algunas reflexiones sobre el libro Nota[N]Roja, señala que se constituye en una guía mínima para periodistas de seguridad pública y justicia. En el se conceptualizan temas como absolución, acusación penal, daño moral querella, menores infractores, se aclaran y desarrollan temas de periodismo como autorregulación, cláusula de conciencia, derecho a la réplica, noticiabilidad, verificación de la información, etc.

  14. Fílon de Alexandria, Flaco: tradução, introdução e notas

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    Faia, Tatiana José Rodrigues

    2010-01-01

    Tese de mestrado, Estudos Clássicos, Universidade de Lisboa, Faculdade de Letras, 2011 Esta monografia consiste numa tradução de Flaco de Fílon de Alexandria, a partir do texto fixado por S. Reiter (Reimer, Berlim, 1915), acompanhada por uma introdução e notas. Abstract: The present monography consists in a translation of Philo of Alexandria's treatise Flaccus, based upon the text established by S. Reiter (Reimer, Berlin, 1915), accompanied by an introduction and notes

  15. Pseudo-obstrucción intestinal por miopatía visceral esporádica Intestinal pseudo-obstruction due to sporadic visceral myopathy

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    Pedro Montalvo

    2004-12-01

    Full Text Available Se presenta el caso infrecuente de un paciente con miopatía visceral esporádica y afectación de la totalidad del tracto gastrointestinal y de la vía urinaria. La miopatía visceral es una forma de pseudo-obstrucción intestinal crónica idiopática caracterizada por degeneración vacuolar, atrofia y fibrosis de la capa muscular propia de la pared intestinal, sin células inflamatorias. Se puede presentar en niños y adolescentes afectando la musculatura visceral digestiva y urinaria. La manifestación familiar se encuentra en aproximadamente el 30% de los casos y se transmite de forma autosómica recesiva en la mayoría de las familias. Es fundamental descartar causas secundarias de pseudo-obstrucción intestinal crónica y la realización de biopsia de todo el espesor de la pared gastrointestinal para poder arribar al diagnóstico. El tratamiento quirúrgico sólo es eficaz en los que tienen afectación de porciones aisladas del tubo digestivo.We report an unusual case of a patient with sporadic visceral myopathy and involvement of the entire gastrointestinal and urinary tract. Visceral myopathy is a form of chronic idiophatic intestinal pseudo-obstruction characterized by vacuolar degeneration, atrophy and fibrosis of the intestinal propia muscle layer without inflammatory cells. It can be found in childhood and adolescence affecting the gastrointestinal and urinary visceral muscle. The familial occurrence can be found in about 30% of cases and the mode of transmission is autosomal recessive in most families. It is crucial to exclude secondary forms of chronic intestinal pseudo-obstruction and to obtain full thickness intestinal biopsy for the diagnosis. Surgical treatment is only beneficial in cases with isolated segmental involvement of the gastrointestinal tract.

  16. Distribution and Impact of the Visceral Fat Area in Patients With Colorectal Cancer

    OpenAIRE

    Yu, Hyeon; Joh, Yong-Geul; Son, Gyung-Mo; Kim, Hyun-Sung; Jo, Hong-Jae; Kim, Hae-Young

    2016-01-01

    Purpose The purposes of this study were to investigate the distribution of the visceral fat area (VFA) and general obesity and to compare visceral and general obesity as predictors of surgical outcomes of a colorectal cancer resection. Methods The prospectively collected data of 102 patients with preoperatively-diagnosed sigmoid colon or rectal cancer who had undergone a curative resection at Pusan National University Yangsan Hospital between April 2011 and September 2012 were reviewed retros...

  17. Standardization of dot-enzyme-linked immmunosorbent assay for the diagnosis of bovine visceral schistosomiasis

    OpenAIRE

    Kommu Sudhakar; Sreenivasa Murthy, G. S.; Gaddam Rajeshwari

    2017-01-01

    Aim: Bovine visceral schistosomiasis has been reported as an important disease entity as it affects animal health, productivity, causes economic losses due to liver condemnation, and produces a high morbidity. This study was conducted to standardize an easy, reliable dot-enzyme-linked immmunosorbent assay (ELISA) for the diagnosis of visceral schistosomiasis caused by Schistosoma spindale and to know the prevalence rate in and around Hyderabad. Materials and Methods: A dot-ELISA was stand...

  18. SLC11A1 polymorphisms and susceptibility to visceral leishmaniasis in Moroccan patients.

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    Ejghal, Rajaâ; Hida, Moustapha; Idrissi, Mona Lakhdar; Hessni, Aboubaker El; Lemrani, Meryem

    2014-12-01

    Human visceral leishmaniasis is endemic in the Mediterranean basin. Since most infections are sub-clinical or asymptomatic, host genetics can provide concrete evidence in determining disease outcome. SLC11A1/NRAMP1 is a candidate gene that may be related to host susceptibility versus resistance to intracellular pathogens. This study aimed to determine possible association of SLC11A1 polymorphisms with visceral leishmaniasis among Moroccan children. A total of 106 children who developed visceral leishmaniasis due to Leishmania infantum were enrolled in this study. The control group was composed of 137 unrelated children, 97 asymptomatic subjects (DTH+) and 42 healthy individuals (DTH) who had no evidence of present or past infection. Four polymorphisms were studied by PCR-RFLP and sequencing: (GT)n microsatellite in the 5' exon 1; silent substitutions 469+14G/C in intron 4; amino acid substitution D543N in exon 15 and 823C/T polymorphism in exon 8. Thereafter, the frequencies of genotypes, alleles and haplotypes were estimated. Two polymorphisms were each significantly associated in the genotypes with visceral leishmaniasis: 823C/T in exon 8 and D543N in exon 15 when comparing visceral leishmaniasis and DTH+ groups. The results of haplotype frequencies suggested an evidence of association with resistance to visceral leishmaniasis for the "286GTG" and "288GCA" haplotypes, whereas, the "286GCG" haplotype appears to increase the risk to visceral leishmaniasis susceptibility.Our data provide insights into the possible role of SLC11A1 variation in visceral leishmaniasis susceptibility. These results must be regarded as preliminary but suggestive that further study with larger populations is worthwhile. Copyright © 2014 Elsevier B.V. All rights reserved.

  19. Associations of visceral fat, physical activity and muscle strength with the metabolic syndrome.

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    Bisschop, Charlotte N Steins; Peeters, Petra H M; Monninkhof, Evelyn M; van der Schouw, Yvonne T; May, Anne M

    2013-10-01

    We investigated the association of visceral fat with the metabolic syndrome (MetS) and its separate components; the associations of both physical activity and muscle strength with the MetS and its separate components independent of visceral fat. Furthermore, we studied these associations within participants with low and high amounts of visceral fat. 400 men (aged 40-80 years) were recruited into a cross-sectional study. Logistic regression models were used to study the individual associations in all participants (OR). The associations of physical activity (active vs inactive) and muscle strength (high vs low) within participants with low and high levels of visceral fat (assessed by ultrasonography) were tested using Univariate Analysis of Variance (difference in mean levels of the separate components of MetS) and logistic regression (risk on MetS). High levels of visceral fat were significantly associated with increased risk of MetS (OR 1.7 95%CI 1.5;1.9) and its separate components (pphysical activity or muscle strength, neither within men with low or high levels of visceral fat. High body fat levels were associated with an unhealthier metabolic risk profile and a higher risk of the MetS. Our cross-sectional data do not indicate associations for physical activity or for muscle strength with the MetS independent of visceral fat. Also no differential associations of physical activity or muscle strength in men with low or high levels of visceral fat were found. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

  20. Visceral leishmaniasis with cutaneous symptoms in a patient treated with infliximab followed by fatal consequences.

    Science.gov (United States)

    Juzlova, Katerina; Votrubova, Jana; Kacerovska, Denisa; Lukas, Milan; Bortlik, Martin; Rohacova, Hana; Nohynkova, Eva; Vojackova, Nadezda; Fialova, Jorga; Hercogova, Jana

    2014-01-01

    Leishmaniasis is an infectious disease caused by parasitic flagellates of the genus Leishmania. The authors present a case of 44-year-old man with Crohn's disease treated successfully with infliximab. This case report shows rare visceral leishmaniasis with cutaneous symptoms in an immunocompromised patient. Skin manifestations may occur before or after the visceral infection and they are often diverse. © 2013 Wiley Periodicals, Inc.

  1. The association of visceral adiposity with cardiovascular events in patients with peripheral artery disease.

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    Oliver Cronin

    Full Text Available BACKGROUND: Previous studies have suggested that patients with peripheral artery disease (PAD suffer from a high incidence of cardiovascular events (CVE. Visceral adiposity has been implicated in promoting CVEs. This study aimed to assess the association of relative visceral adipose volume with incident cardiovascular events in patients with peripheral artery disease. METHODS: This was a prospective cohort study including 260 patients with PAD who presented between 2003 and 2012. Cases were patients with diagnosed PAD including symptomatic lower limb athero-thrombosis and asymptomatic abdominal aortic aneurysm. All patients underwent computed tomography angiography (CTA. Abdominal visceral to total adipose volume ratio (relative visceral adipose volume was estimated from CTAs using a previously validated workstation protocol. Cardiovascular risk factors were recorded at entry. The association of visceral adiposity with major CVEs (death, non-fatal myocardial infarction or stroke was examined using Kaplan Meier and Cox proportional hazard analyses. RESULTS: A total of 92 major CVEs were recorded in 76 patients during a median follow-up of 2.8 (IQR 1.2 to 4.8 years, including myocardial infarction (n = 26, stroke (n = 10 and death (n = 56. At 3 years the incidence of major CVEs stratified by relative visceral adipose volume quartiles were 15% [Quartile (Q 1], 17% (Q2, 11% (Q3 and 15% (Q4 (P = 0.517. Relative visceral adipose volume was not associated with major CVEs after adjustment for other risk factors. CONCLUSION: This study suggests that visceral adiposity does not play a central role in the predisposition for major CVEs in patients with PAD.

  2. Measurement of visceral fat by abdominal bioelectrical impedance analysis is beneficial in medical checkup.

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    Shoji, Kentaro; Maeda, Kazuhisa; Nakamura, Tadashi; Funahashi, Tohru; Matsuzawa, Yuji; Shimomura, Iichiro

    2008-12-01

    In addition to the waist circumference (WC) measurement, an accurate measurement of visceral fat is very important in terms of the pathology associated with the metabolic syndrome. The only method available for an accurate measurement of a visceral fat area (VFA) is abdominal computed tomography (CT). To overcome this limitation, we estimated VFA using abdominal bioelectrical impedance analysis (BIA) with accuracy comparable to that of abdominal CT, and investigated the relationship between VFA and risk factors of the metabolic syndrome. Because abdominal BIA detects excess visceral fat accumulation that cannot be detected by a measurement of WC, the characteristics of the subjects who were identified as having excessive visceral fat using BIA, but not WC, were examined. Abdominal BIA was used to estimate VFA in male subjects (n = 1803) (age: 48 ± 10 years, body mass index: 23.9 ± 3.0 kg/m(2)) who provided informed consent. The prevalence of the metabolic syndrome diagnosed based on the diagnostic criteria established by the Japanese Society of Internal Medicine was 18%. Among the risk factors of the metabolic syndrome, WC was significantly correlated with triglycerides (TG), but estimated VFA was correlated with blood pressure and HDL-cholesterol (HDL-C), in addition to TG. Abdominal BIA detected excess visceral fat accumulation in 3% of subjects for whom WC had detected none. Elevated blood pressure, TG, and fasting plasma glucose and decreased HDL-C were also noted in these subjects, and the values of these risk factors were comparable to those of subjects in whom visceral fat accumulation was detected using WC. Abdominal BIA is a simple and safe method that is superior to WC for the detection of excessive visceral fat accumulation. Therefore, abdominal BIA will likely be used clinically to detect excessive accumulation of visceral fat, which is an important predictor of the metabolic syndrome. © 2008 Asian Oceanian Association for the Study of Obesity

  3. Evaluation of Nephroprotective and Immunomodulatory Activities of Antioxidants in Combination with Cisplatin against Murine Visceral Leishmaniasis

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    Sharma, Meenakshi; Sehgal, Rakesh; Kaur, Sukhbir

    2012-01-01

    BACKGROUND: Most available drugs against visceral leishmaniasis are toxic, and growing limitations in available chemotherapeutic strategies due to emerging resistant strains and lack of an effective vaccine against visceral leishmaniasis deepens the crisis. Antineoplastic drugs like miltefosine have in the past been effective against the parasitic infections. An antineoplastic drug, cisplatin (cis-diamminedichloroplatinum II; CDDP), is recognized as a DNA-damaging drug which also induces alte...

  4. Vulnerability to the transmission of human visceral leishmaniasis in a Brazilian urban area

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    Celina Roma Sánchez de Toledo

    Full Text Available ABSTRACT OBJECTIVE To analyze the determinants for the occurrence of human visceral leishmaniasis linked to the conditions of vulnerability. METHODS This is an ecological study, whose spatial analysis unit was the Territorial Analysis Unit in Araguaína, State of Tocantins, Brazil, from 2007 to 2012. We have carried out an analysis of the sociodemographic and urban infrastructure situation of the municipality. Normalized primary indicators were calculated and used to construct the indicators of vulnerability of the social structure, household structure, and urban infrastructure. From them, we have composed a vulnerability index. Kernel density estimation was used to evaluate the density of cases of human visceral leishmaniasis, based on the coordinates of the cases. Bivariate global Moran’s I was used to verify the existence of spatial autocorrelation between the incidence of human visceral leishmaniasis and the indicators and index of vulnerability. Bivariate local Moran’s I was used to identify spatial clusters. RESULTS We have observed a pattern of centrifugal spread of human visceral leishmaniasis in the municipality, where outbreaks of the disease have progressively reached central and peri-urban areas. There has been no correlation between higher incidences of human visceral leishmaniasis and worse living conditions. Statistically significant clusters have been observed between the incidences of human visceral leishmaniasis in both periods analyzed (2007 to 2009 and 2010 to 2012 and the indicators and index of vulnerability. CONCLUSIONS The environment in circumscribed areas helps as protection factor or increases the local vulnerability to the occurrence of human visceral leishmaniasis. The use of methodology that analyzes the conditions of life of the population and the spatial distribution of human visceral leishmaniasis is essential to identify the most vulnerable areas to the spread/maintenance of the disease.

  5. Role of transient receptor potential melastatin 2 (TRPM2) channels in visceral nociception and hypersensitivity.

    Science.gov (United States)

    Matsumoto, Kenjiro; Takagi, Kanako; Kato, Atsumi; Ishibashi, Takuya; Mori, Yasuo; Tashima, Kimihito; Mitsumoto, Atsushi; Kato, Shinichi; Horie, Syunji

    2016-11-01

    Transient receptor potential melastatin 2 (TRPM2) is a thermosensitive, Ca(2+)-permeable cation channel. TRPM2 contributes to the pathogenesis of inflammatory bowel disease, and inflammatory and neuropathic pain. We hypothesized that TRPM2 is important for visceral nociception and the development of visceral hypersensitivity. Therefore, we investigated the expression of TRPM2 channels and their involvement in visceral nociception in normal physiology and under pathological conditions that cause visceral hypersensitivity in rats. TRPM2 immunoreactivities were detected in the mucosa and muscle layer of the rat gastrointestinal tract. TRPM2 immunopositive cell bodies were almost completely co-localized with calretinin- and NeuN-positive cells in the myenteric plexus. We found that the majority of the TRPM2-immunoreactive cells were double-labeled with the retrograde marker fluorogold in lumbar 6/sacral 1 dorsal root ganglia (DRG), indicating that TRPM2 is expressed in spinal primary afferents innervating the distal colon. Subtypes of TRPM2-immunopositive DRG neurons were labeled by the A-fiber marker NF200, the C-fiber marker IB4, substance P, calcitonin gene-related peptide, or P2X3 receptor. We found that oral administration of the TRPM2 inhibitor econazole (30mg/kg) reduced the visceromotor response (VMR) to noxious colorectal distention (CRD) at 80mmHg in control rats. Expression of TRPM2 in the mucosa of the distal colon was increased in a trinitrobenzene sulfonic acid-induced colitis model. The VMR to CRD significantly increased in colitis model rats compared with control rats at 40, 60, and 80mmHg. Econazole restored visceral hypersensitivity to the control level. Furthermore, TRPM2-deficient mice showed significantly attenuated trinitrobenzene sulfonic acid induced visceral hypersensitivity compared with wild-type mice. In conclusion, TRPM2 channels contribute to visceral nociception in response to noxious stimuli under normal conditions and visceral

  6. Epidemiología molecular de epizootias de rabia en Colombia, 1994-2002: evidencia de rabia humana y canina asociada a quirópteros.

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    Andrés Páez

    2003-03-01

    Full Text Available Tres brotes de rabia canina han sido informados en Colombia durante las pasadas dos décadas, uno de los cuales aún ocurre en la Región Caribe. Los otros dos ocurrieron en el departamento de Arauca y en la Región Central (departamentos de Boyacá y Cundinamarca hasta 1997. Con la finalidad de investigar las relaciones filogenéticas existentes entre los virus rábicos aislados en las regiones mencionadas, se empleó la técnica de RT-PCR para obtener fragmentos de ADN de 902 nucleótidos complementarios a una región del ARN rábico codificante para el endodominio de la proteína G, para una parte de la proteína L, fragmentos que, además, contienen la región intergénica no codificante G-L. Los amplificados fueron secuenciados y agrupados en árboles filogenéticos. Los resultados mostraron la existencia de tres grupos de virus. Los virus rábicos pertenecientes a la variante genética colombiana I fueron aislados exclusivamente en el departamento de Arauca y la Región Central colombiana hasta 1997 y, aparentemente, se encuentran extintos. Los virus rábicos pertenecientes a la variante genética colombiana II fueron aislados exclusivamente en la Región Caribe, en donde actualmente su tranmisión continúa. Un tercer grupo se compone de variantes rábicas originarias de quirópteros, que fueron aisladas de dos murciélagos insectívoros, tres perros y un humano. Con este trabajo se estableció una asociación entre los quirópteros y la rabia en perros y humanos en Colombia, lo cual los muestra como reservorios de importancia en salud pública.

  7. Sleep and birthweight predict visceral adiposity in overweight/obese children.

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    Sokolovic, N; Kuriyan, R; Kurpad, A V; Thomas, T

    2013-06-01

    Visceral adiposity poses significant consequences for long-term health and it is important to identify methods that can be used to prevent fat deposition in visceral adipose tissue. To identify the factors contributing to differential fat distribution in overweight/obese children. Demographic, dietary and lifestyle factors potentially associated with increased visceral adipose tissue in overweight and obese South-Indian children aged 3 to 16 years. The diagnosis of visceral obesity was based on the waist-to-height ratio (WHtR) cut-off value of 0.5. Exposure variables with statistically different distributions in the two WHtR categories, when examined by Mann-Whitney and chi-square tests, were used to develop a binary logistic regression model of visceral adiposity. Increased birthweight and higher sleep duration were significant predictors of having a healthy WHtR, with odds ratios of 1.30 and 1.26 respectively. Early programming effects associated with low birthweight and current sleep deprivation could promote the storage of excess fat as visceral adipose tissue in overweight and obese children. © 2013 The Authors. Pediatric Obesity © 2013 International Association for the Study of Obesity.

  8. Early life experience shapes the functional organization of stress-responsive visceral circuits.

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    Rinaman, Linda; Banihashemi, Layla; Koehnle, Thomas J

    2011-09-26

    Emotions are closely tied to changes in autonomic (i.e., visceral motor) function, and interoceptive sensory feedback from body to brain exerts powerful modulatory control over motivation, affect, and stress responsiveness. This manuscript reviews evidence that early life experience can shape the structure and function of central visceral circuits that underlie behavioral and physiological responses to emotive and stressful events. The review begins with a general discussion of descending autonomic and ascending visceral sensory pathways within the brain, and then summarizes what is known about the postnatal development of these central visceral circuits in rats. Evidence is then presented to support the view that early life experience, particularly maternal care, can modify the developmental assembly and structure of these circuits in a way that impacts later stress responsiveness and emotional behavior. The review concludes by presenting a working hypothesis that endogenous cholecystokinin signaling and subsequent recruitment of gastric vagal sensory inputs to the caudal brainstem may be an important mechanism by which maternal care influences visceral circuit development in rat pups. Early life experience may contribute to meaningful individual differences in emotionality and stress responsiveness by shaping the postnatal developmental trajectory of central visceral circuits. Copyright © 2011 Elsevier Inc. All rights reserved.

  9. Role of sympathetic nervous system in rat model of chronic visceral pain.

    Science.gov (United States)

    Gil, D W; Wang, J; Gu, C; Donello, J E; Cabrera, S; Al-Chaer, E D

    2016-03-01

    Changes in central pain modulation have been implicated in generalized pain syndromes such as irritable bowel syndrome (IBS). We have previously demonstrated that reduced descending inhibition unveils a role of sympathoneuronal outflow in decreasing peripheral sensory thresholds, resulting in stress-induced hyperalgesia. We investigated whether sympathetic nervous system (SNS) exacerbation of pain sensation when central pain inhibition is reduced is relevant to chronic pain disorders using a rat colon irritation (CI) model of chronic visceral hypersensitivity with hallmarks of IBS. Rats were treated to a series of colorectal balloon distensions (CRD) as neonates resulting in visceral and somatic hypersensitivity and altered stool function that persists into adulthood. The visceral sensitivity was assessed by recording electromyographic (EMG) responses to CRD. Somatic sensitivity was assessed by paw withdrawal thresholds to radiant heat. The effects on the hypersensitivity of (i) inhibiting sympathoneuronal outflow with pharmacological and surgical interventions and (ii) enhancing the outflow with water avoidance stress (WAS) were tested. The alpha2-adrenergic agonist, clonidine, and the alpha1-adrenergic antagonist, prazosin, reduced the visceral hypersensitivity and WAS enhanced the pain. Chemical sympathectomy with guanethidine and surgical sympathectomy resulted in a loss of the chronic visceral hypersensitivity. The results support a role of the SNS in driving the chronic visceral and somatic hypersensitivity seen in CI rats. The findings further suggest that treatments that decrease sympathetic outflow or block activation of adrenergic receptors on sensory nerves could be beneficial in the treatment of generalized pain syndromes. © 2015 John Wiley & Sons Ltd.

  10. Influence of Visceral Adiposity on Cardiovascular Autonomic Neuropathy in Patients with Type 2 Diabetes Mellitus

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    Eun-Hee Jang

    2012-08-01

    Full Text Available BackgroundThe aim of this study was to investigate the influences of visceral adiposity on cardiovascular autonomic neuropathy (CAN in patients with type 2 diabetes mellitus.MethodsTwo hundred eleven patients with type 2 diabetes participated in this study. Anthropometric and metabolic parameters were measured, and the visceral fat area was assessed using computed tomography. CAN was diagnosed using a cardiovascular reflex test. We analyzed the correlation between the visceral fat area and each parameter in this test.ResultsThe mean age, body mass index (BMI, and duration of diabetes of the study population were 60±14 years (mean±standard deviation, 25.1±4.2 kg/m2, and 12.3±8.9 years, respectively. The visceral fat area showed positive correlations with age, BMI, waist circumference, and subcutaneous fat area. There was no statistically significant difference in the cardiovascular reflex test outcome between genders. Univariate linear regression analysis showed that an increased visceral fat area diminished good heart rate response to a Valsalva maneuver (R2=4.9%, P=0.013 in an unadjusted model, but only in women. This statistical association was preserved after adjusting for age and BMI (R2=9.8%, P=0.0072.ConclusionThe results of this study suggest that visceral adiposity contributes to an autonomic imbalance to some degree, as demonstrated by the impaired cardiovascular reflex test among women with type 2 diabetes.

  11. Arteriolar function in visceral adipose tissue is impaired in human obesity.

    Science.gov (United States)

    Farb, Melissa G; Ganley-Leal, Lisa; Mott, Melanie; Liang, Yanmei; Ercan, Bahadir; Widlansky, Michael E; Bigornia, Sherman J; Fiscale, Antonino J; Apovian, Caroline M; Carmine, Brian; Hess, Donald T; Vita, Joseph A; Gokce, Noyan

    2012-02-01

    The purpose of this study was to characterize the relationship between adipose tissue phenotype and depot-specific microvascular function in fat. In 30 obese subjects (age 42±11 years, body mass index 46±11 kg/m(2)) undergoing bariatric surgery, we intraoperatively collected visceral and subcutaneous adipose tissue and characterized depot-specific adipose phenotypes. We assessed vasomotor function of the adipose microvasculature using videomicroscopy of small arterioles (75-250 μm) isolated from different fat compartments. Endothelium-dependent, acetylcholine-mediated vasodilation was severely impaired in visceral arterioles, compared to the subcutaneous depot (Peffect on severely blunted visceral arteriolar responses. Visceral fat exhibited greater expression of proinflammatory, oxidative stress-related, hypoxia-induced, and proangiogenic genes; increased activated macrophage populations; and had a higher capacity for cytokine production ex vivo. Our findings provide clinical evidence that the visceral microenvironment may be intrinsically toxic to arterial health providing a potential mechanism by which visceral adiposity burden is linked to atherosclerotic vascular disease. Our findings also support the evolving concept that both adipose tissue quality and quantity may play significant roles in shaping cardiovascular phenotypes in human obesity.

  12. Wt1, the mesothelium and the origins and heterogeneity of visceral fat progenitors.

    Science.gov (United States)

    Chau, You-Ying; Hastie, Nick

    2015-01-01

    One major gap in adipocyte biology has been a lack of understanding of the developmental origins of the different visceral white adipose tissue (WAT) depots and subcutaneous WAT. In a recent study we showed that most visceral WAT but no subcutaneous WAT arises from cells expressing the Wilms' tumor 1 (Wt1) gene late in mouse gestation.(1) Wt1 continues to be expressed in visceral WAT progenitors into adult life. We also showed that visceral WAT is lined by a mesothelium and provided evidence that this structure is the source of adipocytes. Our study also adds to the growing body of evidence that there is heterogeneity in the visceral progenitors, such that there are Wt1-expressing and non-expressing subsets, the relative proportions of which vary between depots. This raises the enticing prospect that the adipocytes arising from these progenitor subsets may have different properties and our preliminary data support this notion. Finally, evidence from our study and one from Spiegelman's group(2) suggests that Wt1 is not just a marker but regulates visceral WAT identity and the progenitor population. We discuss the implications of this work and some of the questions and future directions that arise from it.

  13. Diagnóstico de leishmaniose canina na área urbana do município de Cuiabá, Estado de Mato Grosso, Brasil

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    Saulo Teixeira de Moura

    1999-01-01

    Full Text Available Iniciou-se um estudo sobre a ocorrência de leishmaniose visceral e tegumentar em cães da área urbana de Cuiabá, Estado de Mato Grosso. No período de agosto de 1997 a julho de 1998, foram coletadas amostras de sangue de 800 cães, de diferentes bairros de Cuiabá, escolhidos aleatoriamente, e remetidos ao Serviço de Parasitologia do Instituto Municipal de Medicina Veterinária Jorge Vaitsman, no Rio de Janeiro, para exame pelo teste de imunofluorescência indireta. Foram processadas preliminarmente 62 amostras de soro, das quais 64,5% (40 amostras estavam positivas para leishmaniose. Quanto às titulações encontradas nos soros reagentes, 92,5% (37 amostras apresentaram títulos acima de 1:160, sugerindo forte suspeita da presença de leishmaniose visceral. Deste total, 72,5% (29 amostras dos soros positivos são provenientes de cães da região Sul-Leste e 27,5% (11 amostras da região Centro-Leste de Cuiabá. Este é o primeiro registro da detecção de cães sorologicamente positivos para leishmaniose em área urbana do Estado de Mato Grosso.

  14. Sobre los libros de oro del siglo XVI : notas críticas

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    Teodoro Martín Martín

    1990-01-01

    Full Text Available De sobra es conocido que los Libros de Oro, que aparecen en el siglo XVI en el marco de la renovación pedagógica que introdujo el Humanismo y el Renacimiento, son colecciones de pensamientos, sentencias, máximas y proverbios morales entresacados de las obras de los mejores filósofos estoicos de la Antigüedad Clásica. Sin embargo, poco más es lo que se conoce de ellos fuera del marco de la Historia de la Literatura. Incidentalmente, al ocuparse de la preceptiva de príncipes, algo se ha dicho sobre los textos áureos de la vida, si bien de forma tangencial. Consciente de esta laguna historiográfica voy a redactar las siguientes notas críticas, que no tienen otro objeto más que colaborar en el debate que historiadores, filósofos, pedagogos y literatos han de sostener sobre el tema. Nuestro país aportó al pensamiento educativo del siglo xvi dos figuras señeras: Luis Vives y Pedro Simón Abril. Pero su obra quedó en el plano teórico por cuanto carecieron de capacidad u oportunidad política para implantar sus doctrinas en la sociedad española. Alguna posibilidad tuvo el segundo de los autores citados, pero las mismas se quedaron en forma de memoriales o apuntamientos dirigidos al Rey Felipe II.

  15. Recent understanding in the treatment of visceral leishmaniasis.

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    Rosenthal E

    2003-01-01

    Full Text Available Visceral leishmaniasis (VL is a severe disease associated with infection of the reticuloendothelial system by Leishmania species. The infection is acquired through sandfly bites. Recent large scale epidemics of VL in east Africa and India and the emergence of a HIV epidemic make VL a priority for the World Health Organization. Pentavalent antimonials have been cornerstone of treatment for the last six decades. The appearance of antimonial-resistance and the development of lipid formulations of amphotericin B have changed the pattern of VL treatment. Within the past five years, miltefosine has been demonstrated as the first effective and safe oral treatment against VL. The price of miltefosine is yet to be determined. However, miltefosine will certainly be cheaper than lipid formulations of amphotericin B, which are beyond the financial capacity of the poor countries. Because it can be administered orally, miltefosine is suited for the treatment of large number of patients who get affected during epidemics, particularly in regions where the parasites are resistant to the currently used agents. Here, we recommend different treatment schedules according to the resistance pattern and the region-specific socio-economical and cultural factors.

  16. Live Vaccination Tactics: Possible Approaches for Controlling Visceral Leishmaniasis

    Science.gov (United States)

    Saljoughian, Noushin; Taheri, Tahareh; Rafati, Sima

    2014-01-01

    Vaccination with durable immunity is the main goal and fundamental to control leishmaniasis. To stimulate the immune response, small numbers of parasites are necessary to be presented in the mammalian host. Similar to natural course of infection, strategy using live vaccine is more attractive when compared to other approaches. Live vaccines present the whole spectrum of antigens to the host immune system in the absence of any adjuvant. Leishmanization was the first effort for live vaccination and currently used in a few countries against cutaneous leishmaniasis, in spite of their obstacle and safety. Then, live attenuated vaccines developed with similar promotion of creating long-term immunity in the host with lower side effect. Different examples of attenuated strains are generated through long-term in vitro culturing, culturing under drug pressure, temperature sensitivity, and chemical mutagenesis, but none is safe enough and their revision to virulent form is possible. Attenuation through genetic manipulation and disruption of virulence factors or essential enzymes for intracellular survival are among other approaches that are intensively under study. Other designs to develop live vaccines for visceral form of leishmaniasis are utilization of live avirulent microorganisms such as Lactococcus lactis, Salmonella enterica, and Leishmania tarentolae called as vectored vaccine. Apparently, these vaccines are intrinsically safer and can harbor the candidate antigens in their genome through different genetic manipulation and create more potential to control Leishmania parasite as an intracellular pathogen. PMID:24744757

  17. Exenatide with Metformin Ameliorated Visceral Adiposity and Insulin Resistance

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    Xuan Du

    2018-01-01

    Full Text Available Background. To study the effectiveness of exenatide with metformin and sequential treatment with exenatide and glargine added to metformin and their influence on insulin sensitivity and adipose distribution. Methods. 20 newly diagnosed obese type 2 diabetic patients were enrolled, and 2-month washout treatment of metformin, 6-month exenatide treatment, and 6-month glargine treatment were administrated sequentially accompanied with previous metformin. Glucolipid metabolic parameters were compared among groups. Adipose distribution was quantified with computerized tomography according to anatomy, dividing into visceral adipose tissue (VAT and subcutaneous adipose tissue (SAT, adding up to total adipose tissue (TAT. Results. The 6-month exenatide treatment dramatically ameliorated the glucose and lipid profile, improved insulin sensitivity, and mainly decreased VAT and also the ratio of VAT/SAT (RVS. The following 6-month glargine treatment increased VAT. The whole 12-month sequential treatment with exenatide and glargine added to metformin basically improved the insulin sensitivity and glucolipid control though VAT rebounded at the end, however without deteriorating the other parameters. Conclusion. Exenatide is an ideal treatment for obese type 2 diabetic patients in the aspect of adipose tissue distribution. Sequential treatment of exenatide and glargine could be an alternative for low-income patients who cannot afford GLP-1 agonist for long time. This trial is registered with ChiCTR-OOC-17013679.

  18. Delayed dynamic abdominal wall closure following multi-visceral transplantation.

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    Iype, Satheesh; Butler, Andrew; Jamieson, Neville; Middleton, Stephen; Jah, Asif

    2014-01-01

    Primary closure of the abdominal wall following intestinal transplantation or multivisceral transplantation could become a challenging problem in a significant number of patients. A 38-year-old woman with familial adenomatous polyposis (FAP) underwent a multi-visceral transplantation for short gut syndrome. She subsequently developed acute graft rejection that proved resistant to conventional treatment. She was relisted and underwent re-transplantation along with kidney transplantation. Abdominal wall closure could not be achieved because of the large size of the graft and bowel oedema. The wound was initially managed with laparostomy followed by insertion of the delayed dynamic abdominal closure (DDAC) device (Abdominal Retraction Anchor - ABRA(®) system). Continuous dynamic traction to the wound edges resulted in gradual approximation and complete closure of the abdominal wound was achieved within 3 weeks. Successful abdominal closure after multivisceral transplantation or isolated intestinal transplantation often requires biological mesh, vascularised flaps or abdominal wall transplantation. DDAC eliminated the need for a prosthetic mesh or skin graft and provided an excellent cosmetic result. Adjustment of the dynamic traction at the bedside minimised the need for multiple returns to the operating theatre. It resulted in a well-healed linear scar without a hernia. Dynamic traction allows delayed closure of laparotomy resulting in strong and cosmetically sound wound healing with native tissue. Copyright © 2014 The Authors. Published by Elsevier Ltd.. All rights reserved.

  19. Diagnosis of visceral leishmaniasis: developments over the last decade.

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    Srividya, Gurumurthy; Kulshrestha, Arpita; Singh, Ruchi; Salotra, Poonam

    2012-03-01

    Diagnostic parameters for visceral leishmaniasis (VL), a potentially fatal parasitic disease caused by Leishmania donovani, have been redefined in the last decade with the development of serological and molecular tests, though a definitive diagnosis still banks on the century-old parasitological methods in many areas. Recombinant antigens have improved performance of serodiagnostic methods. Serology-based tests, rk39 antigen dipstick, and direct agglutination test commonly employed in the field are highly sensitive methods, however, fail to distinguish past infections. Molecular approaches have become increasingly relevant due to remarkable sensitivity, specificity, and flexibility in choice of samples. Quantitative polymerase chain reaction is a highly sensitive and specific tool used in referral labs for detection/assessment of parasite load in VL patients and subsequently in monitoring treatment response to antileishmanial agents. The method displays potential to provide threshold for distinguishing asymptomatics in endemic areas. Currently, improvement in VL diagnostics is required for successful decentralized (point-of-care) testing in field conditions and to detect VL-HIV co-infection. Techniques such as loop-mediated isothermal amplification offer a reliable molecular diagnostic method for field application. The diagnosis based on bioanalytics/biosensors promise frontiers for point-of-care VL detection after adequate standardization. This review summarizes the recent developments in VL diagnostics, drawing attention towards the need for standardization of the diagnostics across the affected regions.

  20. Clinical and hematological manifestations of visceral leishmaniasis in Yemeni children

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    Gamal Abdul Hamid

    2009-03-01

    Full Text Available Objective: In southeast Yemen, visceral leishmaniasis (VL is endemic in Lahj and Abyan and also in Hagga and Sadah, the areas lacking adequate diagnostic facilities. This study describes the clinical and hematological features in 64 cases of childhood VL.Material and Methods: All children below 12 years of age who were managed as inpatient cases from 1 January to 31 December 2005 were included in this study. The diagnosis of VL was established by demonstration of leishmania parasites in bone marrow aspiration. Demographic information, physical signs at presentation and results of complete blood count were recorded and bone marrow aspirations were done for LD bodies. Results: Mean age of the patients was 30 months, and there were 33 females and 31 males. Fever was seen in 100% of children with duration before diagnosis of 56 days. Splenomegaly was present in all cases and hepatomegaly in 84.4%, with mean enlargement of spleen and liver of 9.3 and 3.5 cm, respectively. Mean hemoglobin level, white blood cell and platelet counts were 6.6 g/dl, 3.58x109 /L and 71.7x109 /L, respectively. Absolute neutrophil count was <0.78x109 /L and mean reticulocyte count was 1.7%.Conclusion: Fever, hepatosplenomegaly and pancytopenia were the most common clinical and hematological manifestations in Yemeni children with VL.

  1. Immunohistochemical study of hepatic fibropoiesis associated with canine visceral leishmaniasis.

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    Madeira, Igor M V M; Pereira, Debora M O; Sousa, Aline A; Vilela, Cesar A; Amorim, Izabela F G; Caliari, Marcelo V; Souza, Carolina C; Tafuri, Wagner L

    2016-04-01

    Hepatic fibropoiesis has been confirmed in canine visceral leishmaniasis. In fibrotic disease, hepatic stellate cells (HSC) play an important role in fibropoiesis, undergoing activation by TGF-β to acquire characteristics of myofibroblasts. These cells show extensive capacity for proliferation, motility, contractility, collagen synthesis and extracellular matrix component synthesis. The aim of this work was to identify markers of HSC activation in 10 symptomatic and 10 asymptomatic dogs naturally infected with Leishmania (Leishmania) infantum. Eight uninfected dogs were used as controls. Alpha-actin (α-SMA), vimentin and cytokeratin were investigated by immunohistochemistry as HSC markers. The cytokine TGF-β in tissue was also evaluated by immunohistochemistry. All infected dogs showed higher numbers of reticular fibres than controls. Fibropoiesis found in infected dogs was always associated with the presence of parasites and chronic granulomatous hepatitis. Positive correlation was found among fibropoiesis, parasite tissue load and expression of α-SMA. There was no correlation between fibropoiesis, vimentin and cytokeratin markers. The expression of cytokine TGF-β was higher in infected dogs than in controls, but not significantly different between symptomatic and asymptomatic dogs. These results confirm previous work describing the intense hepatic fibropoiesis in dogs naturally infected with Leishmania infantum, but now associated them with overexpression of TGF-β, where α-SMA may be a superior marker for activated HSC cells in CVL. © 2016 The Authors. International Journal of Experimental Pathology © 2016 International Journal of Experimental Pathology.

  2. Gut chemosensing: interactions between gut endocrine cells and visceral afferents.

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    Raybould, Helen E

    2010-02-16

    Chemosensing in the gastrointestinal tract is less well understood than many aspects of gut mechanosensitivity; however, it is important in the overall function of the GI tract and indeed the organism as a whole. Chemosensing in the gut represents a complex interplay between the function of enteroendocrine (EEC) cells and visceral (primarily vagal) afferent neurons. In this brief review, I will concentrate on a new data on endocrine cells in chemosensing in the GI tract, in particular on new findings on glucose-sensing by gut EEC cells and the importance of incretin peptides and vagal afferents in glucose homeostasis, on the role of G protein coupled receptors in gut chemosensing, and on the possibility that gut endocrine cells may be involved in the detection of a luminal constituent other than nutrients, the microbiota. The role of vagal afferent pathways as a downstream target of EEC cell products will be considered and, in particular, exciting new data on the plasticity of the vagal afferent pathway with respect to expression of receptors for GI hormones and how this may play a role in energy homeostasis will also be discussed. Copyright 2009 Elsevier B.V. All rights reserved.

  3. Interactions between visceral afferent signaling and stimulus processing

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    Hugo D Critchley

    2015-08-01

    Full Text Available Visceral afferent signals to the brain influence thoughts, feelings and behaviour. Here we highlight the findings of a set of empirical investigations in humans concerning body-mind interaction that focus on how feedback from states of autonomic arousal shapes cognition and emotion. There is a longstanding debate regarding the contribution of the body, to mental processes. Recent theoretical models broadly acknowledge the role of (autonomically-mediated physiological arousal to emotional, social and motivational behaviours, yet the underlying mechanisms are only partially characterized. Neuroimaging is overcoming this shortfall; first, by demonstrating correlations between autonomic change and discrete patterns of evoked, and task-independent, neural activity; second, by mapping the central consequences of clinical perturbations in autonomic response and; third, by probing how dynamic fluctuations in peripheral autonomic state are integrated with perceptual, cognitive and emotional processes. Building on the notion that an important source of the brain’s representation of physiological arousal is derived from afferent information from arterial baroreceptors, we have exploited the phasic nature of these signals to show their differential contribution to the processing of emotionally-salient stimuli. This recent work highlights the facilitation at neural and behavioral levels of fear and threat processing that contrasts with the more established observations of the inhibition of central pain processing during baroreceptors activation. The implications of this body-brain-mind axis are discussed.

  4. Visceral leishmaniasis and peritoneal tuberculosis: a case report

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    Zamani A

    2009-03-01

    Full Text Available "nBackground: Leishman Denovani is an obligatory intracellular parasite that is seen such as Leishmanbody or Amustigote in intra reticolo-endothelial system. Leishmanenios is seen as sporadic-endemic or epidemic in many places in the world. In Iran in Fars state and west Azarbayjan is endemic and in other places are in sporadic form and is found in rural areas. "nCase report: A four year-old girl was admitted with visceral Leishmaniasis and Subsequently developed peritoneal tuberculosis. The patient who lived in Dashte- Moghan, complained of abdominal pain and distention and weight loss from 1.5 years ago. The titre of IFA test for leishmansis was 1/1280. Leishman body was seen in bone marrow aspiration specimen. Bone marrow culture for leishmania was negative. The specimen of acsities fluid revealed sero- fibrino- purulent exudate with lymphocyic dominancy (over 90%. No response to classic lishmanisis treatment had been started unless the patient treated with anti tuberculoid regimen.  "nConclusion: The function of the T-helper (Tht lymphocytes will decrease in Kala-azar disease. This is why there is no skin reaction to Manteaux (PPD diagnostic test the patient. The patient have been suffering from long-term malnutrition with its consequent immune defect. There was no evidences of cure in our patient during classic Kala-azar therapy. After she received anti tuberculosis therapy she revealed clinical improvement with Glucantim regimen as well.

  5. Visceral Adiposity Index: An Indicator of Adipose Tissue Dysfunction

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    Marco Calogero Amato

    2014-01-01

    Full Text Available The Visceral Adiposity Index (VAI has recently proven to be an indicator of adipose distribution and function that indirectly expresses cardiometabolic risk. In addition, VAI has been proposed as a useful tool for early detection of a condition of cardiometabolic risk before it develops into an overt metabolic syndrome. The application of the VAI in particular populations of patients (women with polycystic ovary syndrome, patients with acromegaly, patients with NAFLD/NASH, patients with HCV hepatitis, patients with type 2 diabetes, and general population has produced interesting results, which have led to the hypothesis that the VAI could be considered a marker of adipose tissue dysfunction. Unfortunately, in some cases, on the same patient population, there is conflicting evidence. We think that this could be mainly due to a lack of knowledge of the application limits of the index, on the part of various authors, and to having applied the VAI in non-Caucasian populations. Future prospective studies could certainly better define the possible usefulness of the VAI as a predictor of cardiometabolic risk.

  6. Outbreak of autochthonous canine visceral leishmaniasis in Santa Catarina, Brazil

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    Mário Steindel

    2013-04-01

    Full Text Available The present study reports the first outbreak of autochthonous canine visceral leishmaniasis in Florianópolis, Santa Catarina, southern Brazil. Following the report of two cases of CVL, the Control Center of Zoonotic Diseases conducted a serological survey by ELISA and IFAT assays in seven districts of the Santa Catarina Island. Eleven seropositive dogs of autochthonous transmission were used in the present study. Infection by Leishmania sp. was confirmed by parasitological examination of bone marrow, liver, spleen and lymph nodes, culture in Schneider's medium and PCR. Leishmania sp. isolates were characterized by PCR-RFLP and hybridization with specific probes, allowing for the identification of Leishmania infantum. Autochthonous transmission of this disease in an area with high tourist traffic presents a major public health concern and signifies the emergence of an important zoonosis in southern Brazil. Therefore, the implementation of surveillance and control measures is imperative to prevent the spread of the disease among the canine population as well as transmission to the human population.

  7. Effects of enterectomy on postoperative visceral organ glucose exchange.

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    Souba, W W; Roughneen, P T; Goldwater, D L; Reed, R L; Rowlands, B J

    1989-01-01

    The effects of a 60% small-bowel resection on postoperative visceral organ glucose exchange was studied in order to gain further understanding of the role of the intestinal tract as a supplier of gluconeogenic substrate to the liver following operative stress. We determined the flux of glucose across the gastrointestinal tract, liver, and kidneys in 20 postoperative dogs. With enterectomy portal bloodflow and total hepatic bloodflow were diminished by 33% and 25%, respectively. Arterial glucose was slightly lower in the enterectomized group 6 hr following the operation. Intestinal glucose uptake was diminished by more than 50% in the enterectomized dogs (p less than 0.01). Net hepatic glucose release fell from 22 mumole/kg/min to 8 mumole/kg/min (p less than 0.01). In control animals the kidney was an organ of slight glucose uptake while in the enterectomized group, the kidney released glucose at the rate of 4.1 mumole/kg/min (p less than 0.05). The data suggest that the gut is an important supplier of gluconeogenic precursors to the liver which are used to support gluconeogenesis in the postoperative period. The ability of the kidney to accelerate glucose production in this setting suggests that metabolic adaptation and cooperation between organs occurs during organ absence or dysfunction which helps preserve glucose homeostasis.

  8. Reduced cortical thickness associated with visceral fat and BMI.

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    Veit, Ralf; Kullmann, Stephanie; Heni, Martin; Machann, Jürgen; Häring, Hans-Ulrich; Fritsche, Andreas; Preissl, Hubert

    2014-01-01

    Structural brain imaging studies have shown that obesity is associated with widespread reductions in gray matter (GM) volume. Although the body mass index (BMI) is an easily accessible anthropometric measure, substantial health problems are more related to specific body fat compartments, like visceral adipose tissue (VAT). We investigated cortical thickness measures in a group of 72 healthy subjects (BMI range 20-35 kg/m(2), age range 19-50 years). Multiple regression analyses were performed using VAT and BMI as predictors and age, gender, total surface area and education as confounds. BMI and VAT were independently associated with reductions in cortical thickness in clusters comprising the left lateral occipital area, the left inferior temporal cortex, and the left precentral and inferior parietal area, while the right insula, the left fusiform gyrus and the right inferior temporal area showed a negative correlation with VAT only. In addition, we could show significant reductions in cortical thickness with increasing VAT adjusted for BMI in the left temporal cortex. We were able to detect widespread cortical thinning in a young to middle-aged population related to BMI and VAT; these findings show close resemblance to studies focusing on GM volume differences in diabetic patients. This may point to the influence of VAT related adverse effects, like low-grade inflammation, as a potentially harmful factor on brain integrity already in individuals at risk of developing diabetes, metabolic syndromes and arteriosclerosis.

  9. Reduced cortical thickness associated with visceral fat and BMI

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    Ralf Veit

    2014-01-01

    Full Text Available Structural brain imaging studies have shown that obesity is associated with widespread reductions in gray matter (GM volume. Although the body mass index (BMI is an easily accessible anthropometric measure, substantial health problems are more related to specific body fat compartments, like visceral adipose tissue (VAT. We investigated cortical thickness measures in a group of 72 healthy subjects (BMI range 20–35 kg/m2, age range 19–50 years. Multiple regression analyses were performed using VAT and BMI as predictors and age, gender, total surface area and education as confounds. BMI and VAT were independently associated with reductions in cortical thickness in clusters comprising the left lateral occipital area, the left inferior temporal cortex, and the left precentral and inferior parietal area, while the right insula, the left fusiform gyrus and the right inferior temporal area showed a negative correlation with VAT only. In addition, we could show significant reductions in cortical thickness with increasing VAT adjusted for BMI in the left temporal cortex. We were able to detect widespread cortical thinning in a young to middle-aged population related to BMI and VAT; these findings show close resemblance to studies focusing on GM volume differences in diabetic patients. This may point to the influence of VAT related adverse effects, like low-grade inflammation, as a potentially harmful factor on brain integrity already in individuals at risk of developing diabetes, metabolic syndromes and arteriosclerosis.

  10. Perceptions of the population and health professionals regarding visceral leishmaniasis.

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    Carmo, Rose Ferraz; da Luz, Zélia Maria Profeta; Bevilacqua, Paula Dias

    2016-02-01

    Based on theoretical qualitative research reference methodology, this study sought to investigate the perception of visceral leishmaniasis (VL) by social actors directly involved in the prevention and control of the disease. Thirty-eight semi-structured interviews were conducted with residents, focus groups were staged with 18 health workers in an endemic VL area and depositions were collected, which after being processed by content analysis revealed shortcomings and challenges. The population associated VL with dogs, acknowledged their co-responsibility in tackling the disease and demanded information. Health workers identified environmental sanitation as an essential factor for VL prevention. Among the shortcomings, the lack of information about the disease and culpability of the individual because of non-adherence to prevention measures were observed, especially environmental management. Probably, approaches emphasizing the role of the environment as a health promotion agent and the timely definition of specific environmental measures against VL, constitute a prospect for overcoming these shortcomings. The consensus is that the main challenge for enhancing the prevention and control might be the participatory and dialogical construction of these approaches between health professionals and the population.

  11. Multi-cellular rosettes in the mouse visceral endoderm facilitate the ordered migration of anterior visceral endoderm cells.

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    Georgios Trichas

    2012-02-01

    Full Text Available The visceral endoderm (VE is a simple epithelium that forms the outer layer of the egg-cylinder stage mouse embryo. The anterior visceral endoderm (AVE, a specialised subset of VE cells, is responsible for specifying anterior pattern. AVE cells show a stereotypic migratory behaviour within the VE, which is responsible for correctly orientating the anterior-posterior axis. The epithelial integrity of the VE is maintained during the course of AVE migration, which takes place by intercalation of AVE and other VE cells. Though a continuous epithelial sheet, the VE is characterised by two regions of dramatically different behaviour, one showing robust cell movement and intercalation (in which the AVE migrates and one that is static, with relatively little cell movement and mixing. Little is known about the cellular rearrangements that accommodate and influence the sustained directional movement of subsets of cells (such as the AVE within epithelia like the VE. This study uses an interdisciplinary approach to further our understanding of cell movement in epithelia. Using both wild-type embryos as well as mutants in which AVE migration is abnormal or arrested, we show that AVE migration is specifically linked to changes in cell packing in the VE and an increase in multi-cellular rosette arrangements (five or more cells meeting at a point. To probe the role of rosettes during AVE migration, we develop a mathematical model of cell movement in the VE. To do this, we use a vertex-based model, implemented on an ellipsoidal surface to represent a realistic geometry for the mouse egg-cylinder. The potential for rosette formation is included, along with various junctional rearrangements. Simulations suggest that while rosettes are not essential for AVE migration, they are crucial for the orderliness of this migration observed in embryos. Our simulations are similar to results from transgenic embryos in which Planar Cell Polarity (PCP signalling is disrupted

  12. Interleukin-1 beta: a potential link between stress and the development of visceral obesity.

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    Speaker, Kristin J; Fleshner, Monika

    2012-06-27

    A disproportionate amount of body fat within the abdominal cavity, otherwise known as visceral obesity, best predicts the negative health outcomes associated with high levels body fat. Growing evidence suggests that repeated activation of the stress response can favor visceral fat deposition and that visceral obesity may induce low-grade, systemic inflammation which is etiologically linked to the pathogenesis of obesity related diseases such as cardiovascular disease and type 2 diabetes. While the obesity epidemic has fueled considerable interest in these obesity-related inflammatory diseases, surprisingly little research is currently focused on understanding the functions of inflammatory proteins in healthy, non-obese white adipose tissue (WAT) and their possible role in modulating stress-induced shifts in body fat distribution. The current review presents evidence in support the novel hypothesis that stress-evoked interleukin-1 beta (IL-1β) signaling within subcutaneous adipose tissue, when repeatedly induced, contributes toward the development of visceral obesity. It is suggested that because acute stressor exposure differentially increases IL-1β levels within subcutaneous adipose relative to visceral adipose tissue in otherwise healthy, non-obese rats, repeated induction of this response may impair the ability of subcutaneous adipose tissue to uptake energy substrates, synthesize and retain triglycerides, and/or adapt to positive energy balance via hyperplasia. Consequently, circulating energy substrates may be disproportionately shunted to visceral adipose tissue for storage, thus driving the development of visceral obesity. This review establishes the following key points: 1) body fat distribution outweighs the importance of total body fat when predicting obesity-related disease risk; 2) repeated exposure to stress can drive the development of visceral obesity independent of changes in body weight; 3) because of the heterogeneity of WAT composition and

  13. Interleukin-1 beta: a potential link between stress and the development of visceral obesity

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    Speaker Kristin J

    2012-06-01

    Full Text Available Abstract Background A disproportionate amount of body fat within the abdominal cavity, otherwise known as visceral obesity, best predicts the negative health outcomes associated with high levels body fat. Growing evidence suggests that repeated activation of the stress response can favor visceral fat deposition and that visceral obesity may induce low-grade, systemic inflammation which is etiologically linked to the pathogenesis of obesity related diseases such as cardiovascular disease and type 2 diabetes. While the obesity epidemic has fueled considerable interest in these obesity-related inflammatory diseases, surprisingly little research is currently focused on understanding the functions of inflammatory proteins in healthy, non-obese white adipose tissue (WAT and their possible role in modulating stress-induced shifts in body fat distribution. Hypothesis The current review presents evidence in support the novel hypothesis that stress-evoked interleukin-1 beta (IL-1β signaling within subcutaneous adipose tissue, when repeatedly induced, contributes toward the development of visceral obesity. It is suggested that because acute stressor exposure differentially increases IL-1β levels within subcutaneous adipose relative to visceral adipose tissue in otherwise healthy, non-obese rats, repeated induction of this response may impair the ability of subcutaneous adipose tissue to uptake energy substrates, synthesize and retain triglycerides, and/or adapt to positive energy balance via hyperplasia. Consequently, circulating energy substrates may be disproportionately shunted to visceral adipose tissue for storage, thus driving the development of visceral obesity. Conclusions This review establishes the following key points: 1 body fat distribution outweighs the importance of total body fat when predicting obesity-related disease risk; 2 repeated exposure to stress can drive the development of visceral obesity independent of changes in body weight

  14. The Usefulness of Visceral Fat Thickness Measured by Ultrasonography as an Abdominal Obesity Index

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    Kim, Yong Kyun [Dept. of Diagnostic Radiology, Korealife Daejeon Healthcare Center, Daejeon (Korea, Republic of); Han, Man Seok [Dept. of Diagnostic Radiology, Chungnam National University Hospital, Daejeon (Korea, Republic of)

    2008-09-15

    Abdominal obesity with visceral fat accumulation have been known to be intimately associated with the development of metabolic syndrome. Therefore, it is important to estimate the precise amount of visceral fat. Ultrasonography has been reported that it is a simple and noninvasive method for visceral fat evaluation. Purpose of this study is to evaluate the association of ultrasonographic visceral fat thickness, anthropometric indexes, and risk factor of metabolic syndrome, and to investigate the cut-off value of abdominal visceral fat thickness leading to increased risk of metabolic syndrome. The subject included 200 men and 200 women who visited D healthcare center in Daejeon from January to April 2008. The subcutaneous fat thickness and visceral fat thickness were measured by ultrasonograph. As anthropometric index, we measured body mass index, waist circumference and waist/height ratio. As for the risk factor of metabolic syndrome, we measured blood pressure, high density lipoprotein cholesterol, triglyceride and fasting serum glucose. VFT was significantly correlated with waist circumference, (r=0.683/M, r=0.604/F), waist to height ratio (r=0.633/M, r=0.593/F) and BMI (r=0.621/M, r=0.534/F) in both men and women. In addition it was significantly correlated with Systolic blood pressure (r=0.229/M, r=0.232/F), Diastolic blood pressure ((r=0.285/M, r=0.254/F), high density cholesterol (r=-0.254/M, r=-0.254/F), Triglyceride (r=0.475/M, r=0.411/F), and Fasting blood sugar (r=0.158/M, r=0.234/F) in both men and women. The cut-off value of visceral fat thickness leading to the increased risk of metabolic syndrome was 4.58 cm (sensitivity 89.2%, specificity 71.2%) in men and 3.50 cm (sensitivity 61.2% specificity 80.8%) in women respectively. The odds ratio of the risk of metabolic syndrome was dramatically increased with the abdominal visceral fat thickness level over 6 cm in men and 5 cm in women. The visceral fat thickness using ultrasonography was significantly

  15. Nota técnica No. 1: Primer Encuentro Nacional de Comités de Emergencias

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    Araya Araya, Ramón

    2012-07-01

    Full Text Available La presente nota técnica se refiere al Primer Encuentro Nacional de Comités de Emergencia, realizado los días 20, 21 y 22 de marzo del 2012. Se utilizó una metodología participativa, la cual consistió en exposiciones de funcionarios de la Comisión Nacional de Prevención de Riesgos y Atención de Emergencias y de los mismos integrantes de los comités de emergencia, quienes compartieron sus experiencias exitosas. Se informa en la nota técnica que el Encuentro convocó por primera vez a todos los comités regionales y municipales del país. This technical note refers to the First National Meeting of Emergency Committees, held on March 20, 21 and 22, 2012. The methodology used was participative, with presentations from personnel of the National Commission of Risk Prevention and Emergency Response and from the Emergency Committee members. They shared their successful experiences. The technical note informs that the Meeting convened for the first time all the regional and municipal committees of the country

  16. Mujeres delincuentes e imaginarios. Criminología, cine y nota roja en México, 1940-1950

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    MARTHA SANTILLÁN ESQUEDA

    Full Text Available RESUMEN La modernización política y económica impulsada por los gobiernos tras la Revolución Mexicana, repercutió en la apertura de espacios para el desenvolvimiento de mujeres fuera del ámbito doméstico. La especialización de la criminología y la profesionalización de medios de comunicación, ligados a un fuerte conservadurismo, coadyuvaron en la conformación de un notorio temor ante tal fenómeno, asumiendo que ello pudiera provocar en las mujeres el relajamiento de la moral y, en consecuencia, conductas delictivas. El artículo analiza la conformación de imaginarios en torno a la mujer delincuente en un periodo de notorio auge tanto del cine como de la nota roja. Al presentar a las criminales como un ser monstruoso y perverso, imagen que no distaba mucho de las ideas criminológicas, se prolongaban temores existentes en torno a la delincuencia femenina pero también se fortalecía la concepción de una feminidad atada al espacio doméstico. Se estudian filmes, prensa nota roja, diarios de circulación nacional y artículos criminológicos.

  17. Compreensão de leitura: estratégias de tomar notas e da imagem mental

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    Sandra Patrícia Ataíde Ferreira

    Full Text Available Objetivou-se verificar e comparar o efeito do treinamento das estratégias de Tomar Notas e da Imagem Mental sobre a compreensão de leitura entre crianças de oito a 14 anos com dificuldades nesta área, de escolas públicas e particulares. Primeiramente, foram classificadas nos Grupos de Pouca e Muita Dificuldade de Compreensão (GD1 e GD2 e distribuídas em três grupos: dois grupos experimentais (GE1 e GE2 e um grupo controle (GC. Depois, o GE1 utilizou a atividade de Tomar Notas e o GE2, a estratégia da Imagem Mental. O GC não recebeu treinamento, mas realizou a mesma tarefa que os grupos experimentais. Os resultados demonstram um desempenho significativamente melhor do GE1 frente ao GE2 e ao GC. Verificou-se que o GD1 progrediu mais sobre as questões inferenciais do que o GD2. As crianças das escolas públicas foram as mais beneficiadas. Ambas estratégias possibilitaram a emergência de respostas às questões literais e inferenciais.

  18. Compreensão de leitura: estratégias de tomar notas e da imagem mental

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    Ferreira Sandra Patrícia Ataíde

    2002-01-01

    Full Text Available Objetivou-se verificar e comparar o efeito do treinamento das estratégias de Tomar Notas e da Imagem Mental sobre a compreensão de leitura entre crianças de oito a 14 anos com dificuldades nesta área, de escolas públicas e particulares. Primeiramente, foram classificadas nos Grupos de Pouca e Muita Dificuldade de Compreensão (GD1 e GD2 e distribuídas em três grupos: dois grupos experimentais (GE1 e GE2 e um grupo controle (GC. Depois, o GE1 utilizou a atividade de Tomar Notas e o GE2, a estratégia da Imagem Mental. O GC não recebeu treinamento, mas realizou a mesma tarefa que os grupos experimentais. Os resultados demonstram um desempenho significativamente melhor do GE1 frente ao GE2 e ao GC. Verificou-se que o GD1 progrediu mais sobre as questões inferenciais do que o GD2. As crianças das escolas públicas foram as mais beneficiadas. Ambas estratégias possibilitaram a emergência de respostas às questões literais e inferenciais.

  19. Heredabilidad y repetibilidad de la nota de tienta y de lidia en una ganadería de reses bravas en Colombia

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    Durán C. Carlos Vicente

    1995-12-01

    Full Text Available

    A study in the bullfight herd of Ernesto González Caicedo located in the Department of Cauca, Colombia, mostly of Santa Coloma lineage (a strain of bull-fighting cattle, either pure bred or up graded, evaluated between 1963 and 1994. The information arose from 948 standing records of "Nota de Tienta" (a quantitative test given to females to evaluate their potential for the bullfight. The test is used to select breeding darns and also used in males to select the future sires and "Nota de Lidia" (a quantitative test given to bulls to evaluate their overall performance in the bullfight. The Nota de Tienta and Nota de Lidia may not allow to make comparisons due the differences between both practices, but a "t" test to determinate evaluation type and sex effects over the calculate parameters showed them to be comparable (P <0.05. The repeatability was estimated as 0.18, a value inferior to the estimated heritability index h2 = 0.24. The difference may be explained by a bias in the estimated values of temporal and permanent environmental variances resulting from special environmental effects which influence on the behavior of the animal when it is tested.

    Se realizó un estudio en la ganadería de lidia de Ernesto González Caicedo, fundamentalmente de origen Santa Coloma, pura o por cruza absorbente de ganado mexicano, ubicada en el departamento del Cauca, Colombia. La información utilizada constó de 948 Notas de Tienta y de Lidia entre 1963 y 1994. Las notas de Tienta y de Lidia, que por el desarrollo un tanto diferente de las dos prácticas, pueden no permitir comparaciones, resultaron equiparables luego de realizar una prueba de "t" para medir efectos del tipo de evaluación y el sexo sobre la nota obtenida (P<0.05. La repetibilidad estimada para estas variables resultó en rxx= 0.18, un valor inferior al índice de heredabilidad obtenido durante la misma investigación, calculado en h2 = 0.24, a través de diferentes

  20. The Effect of Mini-PEG-Based Spacer Length on Binding and Pharmacokinetic Properties of a 68Ga-Labeled NOTA-Conjugated Antagonistic Analog of Bombesin

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    Zohreh Varasteh

    2014-07-01

    Full Text Available The overexpression of gastrin-releasing peptide receptor (GRPR in cancer can be used for peptide-receptor mediated radionuclide imaging and therapy. We have previously shown that an antagonist analog of bombesin RM26 conjugated to 1,4,7-triazacyclononane-N,N',N''-triacetic acid (NOTA via a diethyleneglycol (PEG2 spacer (NOTA-PEG2-RM26 and labeled with 68Ga can be used for imaging of GRPR-expressing tumors. In this study, we evaluated if a variation of mini-PEG spacer length can be used for optimization of targeting properties of the NOTA-conjugated RM26. A series of analogs with different PEG-length (n = 2, 3, 4, 6 was synthesized, radiolabeled and evaluated in vitro and in vivo. The IC50 values of natGa-NOTA-PEGn-RM26 (n = 2, 3, 4, 6 were 3.1 ± 0.2, 3.9 ± 0.3, 5.4 ± 0.4 and 5.8 ± 0.3 nM, respectively. In normal mice all conjugates demonstrated similar biodistribution pattern, however 68Ga-NOTA-PEG3-RM26 showed lower liver uptake. Biodistribution of 68Ga-NOTA-PEG3-RM26 was evaluated in nude mice bearing PC-3 (prostate cancer and BT-474 (breast cancer xenografts. High uptake in tumors (4.6 ± 0.6%ID/g and 2.8 ± 0.4%ID/g for PC-3 and BT-474 xenografts, respectively and high tumor-to-background ratios (tumor/blood of 44 ± 12 and 42 ± 5 for PC-3 and BT-474 xenografts, respectively were found already at 2 h p.i. of 68Ga-NOTA-PEG3-RM26. Results of this study suggest that variation in the length of the PEG spacer can be used for optimization of targeting properties of peptide-chelator conjugates. However, the influence of the mini-PEG length on biodistribution is minor when di-, tri-, tetra- and hexaethylene glycol are compared.

  1. Erliquiose monocítica canina subclínica, naturalmente adquirida: diagnóstico, aspectos clínico-laboratoriais, envolvimento renal e evolução com o tratamento

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    Alves, Marcelo Augusto Moraes Koury [UNESP

    2013-01-01

    A erliquiose canina é uma doença causada pela bactéria Gram-negativa Ehrlichia canis transmitida pelo carrapato Rhipicephalus sanguineus. A enfermidade é endêmica em muitas regiões do mundo e motivo de investigação científica em muitos países. Independentemente dos muitos avanços sobre a patogênese e o diagnóstico da doença existem poucos conhecimentos sobre o acometimento renal. O presente estudo teve por escopo caracterizar a condição clínico-laboratorial, com enfoque na função renal, de cã...

  2. Identificação das bactérias envolvidas na sepse grave de fêmeas caninas com piometra submetidas a ovário-histerectomia terapêutica

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    Tabatha do Amaral Kalenski

    2012-04-01

    Full Text Available A piometra é uma afecção reprodutiva comum que acomete fêmeas caninas, podendo se agravar e progredir para o quadro de sepse grave e choque séptico. A precocidade da instituição da antibioticoterapia é determinante para um melhor prognóstico. O objetivo deste estudo foi avaliar os principais microrganismos envolvidos nos casos de sepse grave em cadelas acometidas por piometra e submetidas à ovário-histerectomia terapêutica, por meio de realização de hemocultura e cultura da secreção uterina e antibiograma. Foram avaliadas 33 fêmeas caninas e o principal agente envolvido com a sepse grave secundária à piometra foi a Escherichia coli, identificada em 57,57% dos casos. Também foram identificados Staphylococcus sp., com incidência de 9,09%, Citrobacter koseri, Enterobacter cloacae, Enterobacter faecalis, Eduardsiella sp., Klebsiella pneumoniae e Streptococcus sp., com 3,03% de frequência cada. Após a realização do antibiograma pelo método de difusão, os antimicrobianos que apresentaram maior eficácia contra as cepas de Escherichia coli foram a gentamicina, a enrofloxacina, a cefalexina e a associação de amoxicilina com ácido clavulânico, nesta ordem. A cultura da secreção uterina foi mais sensível que a hemocultura para identificação do agente microbiano (p<0,0001. A identificação bacteriana é útil para direcionar a antibioticoterapia empírica mais específica, de acordo com o perfil de sensibilidade, minimizando assim o desenvolvimento de resistência, o custo do tratamento e o risco de reações adversas aos antimicrobianos utilizados.

  3. Lymphatic drainage of lung segments in the visceral pleura: a cadaveric study.

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    Fourdrain, Alex; Lafitte, Sophie; Iquille, Jules; De Dominicis, Florence; Havet, Eric; Peltier, Johann; Bagan, Patrick; Berna, Pascal

    2017-08-19

    Although peribronchial lymphatic drainage of the lung has been well characterized, lymphatic drainage in the visceral pleura is less well understood. The objective of the present study was to evaluate the lymphatic drainage of lung segments in the visceral pleura. Adult, European cadavers were examined. Cadavers with a history of pleural or pulmonary disease were excluded. The cadavers had been refrigerated but not embalmed. The lungs were surgically removed and re-warmed. Blue dye was injected into the subpleural area and into the first draining visceral pleural lymphatic vessel of each lung segment. Twenty-one cadavers (7 males and 14 females; mean age 80.9 years) were dissected an average of 9.8 day postmortem. A total of 380 dye injections (in 95 lobes) were performed. Lymphatic drainage of the visceral pleura followed a segmental pathway in 44.2% of the injections (n = 168) and an intersegmental pathway in 55.8% (n = 212). Drainage was found to be both intersegmental and interlobar in 2.6% of the injections (n = 10). Lymphatic drainage in the visceral pleura followed an intersegmental pathway in 22.8% (n = 13) of right upper lobe injections, 57.9% (n = 22) of right middle lobe injections, 83.3% (n = 75) of right lower lobe injections, 21% (n = 21) of left upper lobe injections, and 85.3% (n = 81) of left lower lobe injections. In the lung, lymphatic drainage in the visceral pleura appears to be more intersegmental than the peribronchial pathway is-especially in the lower lobes. The involvement of intersegmental lymphatic drainage in the visceral pleura should now be evaluated during pulmonary resections (and especially sub-lobar resections) for lung cancer.

  4. Sensory receptors in the visceral pleura: neurochemical coding and live staining in whole mounts.

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    Pintelon, Isabel; Brouns, Inge; De Proost, Ian; Van Meir, Frans; Timmermans, Jean-Pierre; Adriaensen, Dirk

    2007-05-01

    Today, diagnosis and treatment of chest pain related to pathologic changes in the visceral pleura are often difficult. Data in the literature on the sensory innervation of the visceral pleura are sparse. The present study aimed at identifying sensory end-organs in the visceral pleura, and at obtaining more information about neurochemical coding. The immunocytochemcial data are mainly based on whole mounts of the visceral pleura of control and vagally denervated rats. It was shown that innervation of the rat visceral pleura is characterized by nerve bundles that enter in the hilus region and gradually split into slender bundles with a few nerve fibers. Separate nerve fibers regularly give rise to characteristic laminar terminals. Because of their unique association with the elastic fibers of the visceral pleura, we decided to refer to them as "visceral pleura receptors" (VPRs). Cryostat sections of rat lungs confirmed a predominant location on mediastinal and interlobar lung surfaces. VPRs can specifically be visualized by protein gene product 9.5 immunostaining, and were shown to express vesicular glutamate transporters, calbindin D28K, Na+/K+-ATPase, and P2X3 ATP-receptors. The sensory nerve fibers giving rise to VPRs appeared to be myelinated and to have a spinal origin. Because several of the investigated proteins have been reported as markers for sensory terminals in other organs, the present study revealed that VPRs display the neurochemical characteristics of mechanosensory and/or nociceptive terminals. The development of a live staining method, using AM1-43, showed that VPRs can be visualized in living tissue, offering an interesting model for future physiologic studies.

  5. Risk factors for death in children with visceral leishmaniasis.

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    Márcia Jaqueline Alves de Queiroz Sampaio

    Full Text Available BACKGROUND: Despite the major public health importance of visceral leishmaniasis (VL in Latin America, well-designed studies to inform diagnosis, treatment and control interventions are scarce. Few observational studies address prognostic assessment in patients with VL. This study aimed to identify risk factors for death in children aged less than 15 years admitted for VL treatment in a referral center in northeast Brazil. METHODOLOGY/PRINCIPAL FINDINGS: In a retrospective cohort, we reviewed 546 records of patients younger than 15 years admitted with the diagnosis of VL at the Instituto de Medicina Integral Professor Fernando Figueira between May 1996 and June 2006. Age ranged from 4 months to 13.7 years, and 275 (50% were male. There were 57 deaths, with a case-fatality rate of 10%. In multivariate logistic regression, the independent predictors of risk of dying from VL were (adjusted OR, 95% CI: mucosal bleeding (4.1, 1.3-13.4, jaundice (4.4, 1.7-11.2, dyspnea (2.8, 1.2-6.1, suspected or confirmed bacterial infections (2.7, 1.2-6.1, neutrophil count <500/mm³ (3.1, 1.4-6.9 and platelet count <50,000/mm³ (11.7, 5.4-25.1. A prognostic score was proposed and had satisfactory sensitivity (88.7% and specificity (78.5%. CONCLUSIONS/SIGNIFICANCE: Prognostic and severity markers can be useful to inform clinical decisions such as whether a child with VL can be safely treated in the local healthcare facility or would potentially benefit from transfer to referral centers where advanced life support facilities are available. High risk patients may benefit from interventions such as early use of extended-spectrum antibiotics or transfusion of blood products. These baseline risk-based supportive interventions should be assessed in clinical trials.

  6. Epiploic appendagitis: 7-year experience and relationship with visceral obesity.

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    Nugent, James P; Ouellette, Hugue A; O'Leary, D Peter; Khosa, Faisal; Nicolaou, Savvas; McLaughlin, Patrick D

    2017-10-17

    Location, size, and local inflammatory findings in primary epiploic appendagitis (EA) have not been reported outside of small studies. The association between EA and increased adiposity is controversial. The goals of this project are to compare demographics and imaging-based measurements of adiposity between patients with EA and patients with acute abdomen without EA, and to identify CT features of EA. A consecutive sample of acute, primary EA (n = 100), and control (acute abdomen; n = 100) was selected retrospectively. Cases of suspected EA were included if they had the characteristic ovoid fatty mass and hyperattenuated ring sign on CT. Abdominal adipose volume (AAV), visceral adipose area (VAA), and subcutaneous adipose area (SAA) were quantified by CT. Location, size, and frequency of coexisting local inflammatory findings in EA patients were recorded. EA had 60% greater AAV, 117% greater VAA, and 35% greater SAA than control subjects (p < 0.0001). Males composed a great proportion of the EA group (67%) than the control group (41%) (p = 0.0002). Inflamed appendage was found in sigmoid colon in 49% of cases, descending colon in 23%, and right colon in 19%. Peritoneal thickening was frequent (76%) and bowel wall thickening was common (47%). Diverticulosis coexisted incidentally in 28%. EA is associated with increased abdominal adipose tissue. EA can occur in both sexes at any age, but occurs at age 50 on average and more frequently in males. Patient with EA exhibited central hyperdense dot (79%), peritoneal thickening (76%), and bowel wall thickening (47%).

  7. Serum cytokine profile in the subclinical form of visceral leishmaniasis

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    Gama M.E.A.

    2004-01-01

    Full Text Available The factors determining the development or not of visceral leishmaniasis (VL have not been completely identified, but a Leishmania-specific cellular immune response seems to play a fundamental role in the final control of infection. Few studies are available regarding the production of cytokines in the subclinical form of VL, with only the production of IFN-g and TNF-a known. The aim of the present study was to identify immunological markers for the oligosymptomatic or subclinical form of VL. A prospective cohort study was conducted on 784 children aged 0 to 5 years from an endemic area in the State of Maranhão, Brazil, between January 1998 and December 2001. During 30 consecutive months of follow-up, 33 children developed the oligosymptomatic form of the disease and 12 the acute form. During the clinical manifestations, serum cytokine levels were determined in 27 oligosymptomatic children and in nine patients with the acute form using a quantitative sandwich enzyme immunoassay. In the subclinical form of VL, variable levels of IL-2 were detected in 52.3% of the children, IL-12 in 85.2%, IFN-g in 48.1%, IL-10 in 88.9%, and TNF-a in 100.0%, with the last two cytokines showing significantly lower levels than in the acute form. IL-4 was not detected in oligosymptomatic individuals. Multiple discriminant analysis used to determine the profile or combination of cytokines predominating in the subclinical form revealed both a Leishmania resistance (Th1 and susceptibility (Th2 profile. The detection of both Th1 and Th2 cytokine profiles explains the self-limited evolution accompanied by the discrete alterations observed for the subclinical form of VL.

  8. Eco-epidemiology of visceral leishmaniasis in Ethiopia.

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    Gadisa, Endalamaw; Tsegaw, Teshome; Abera, Adugna; Elnaiem, Dia-Eldin; den Boer, Margriet; Aseffa, Abraham; Jorge, Alvar

    2015-07-19

    Visceral leishmaniasis (VL, Kala-azar) is one of the growing public health challenges in Ethiopia with over 3.2 million people at risk and estimated up to 4000 new cases per year. Historically, VL was known as the diseases of the lowlanders; in the lower and upper Kola agro-ecological zones of Ethiopia. The 2005-07 out breaks in highlands of Libo Kemkem and Fogera, in the Woina Degas, that affected thousands and claimed the life of hundreds misdiagnosed as drug resistance malaria marked that VL is no more the problem of the lowlanders. The Kola (lower and upper) and the Woina Dega are the most productive agroecological zones, supporting both the ongoing and planned expansions of large or small scale agriculture and/or agriculture based industries. Thus, the (re)emergence of VL is not only a public health and social problem but also have a direct implication on the country's economy and further development. Thus is high time for its control and/or elimination. Yet, the available data seem incomplete to plan for a cost-effective and efficient VL control strategy: there is a need to update data on vector behaviour in specific ecosystems and the roles of domestic animals need to be ascertained. The effectiveness and social acceptability of available vector control tools need be evaluated. There is a need for identifying animal reservoir(s), or establish the absence of zoonosis in Ethiopia. The planning of prevention of (re)emergence and spread of VL to areas adjacent to endemic foci need be supported with information from spatio-temporal mapping. In affected communities, available data showed that their knowledge about VL is generally very low. Thus, well designed studies to identify risk factors, as well as better tools for social mobilization with the understanding of their knowledge, aptitude and practice towards VL are necessary.

  9. Combined Immune Therapy for the Treatment of Visceral Leishmaniasis.

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    Rebecca J Faleiro

    2016-02-01

    Full Text Available Chronic disease caused by infections, cancer or autoimmunity can result in profound immune suppression. Immunoregulatory networks are established to prevent tissue damage caused by inflammation. Although these immune checkpoints preserve tissue function, they allow pathogens and tumors to persist, and even expand. Immune checkpoint blockade has recently been successfully employed to treat cancer. This strategy modulates immunoregulatory mechanisms to allow host immune cells to kill or control tumors. However, the utility of this approach for controlling established infections has not been extensively investigated. Here, we examined the potential of modulating glucocorticoid-induced TNF receptor-related protein (GITR on T cells to improve anti-parasitic immunity in blood and spleen tissue from visceral leishmaniasis (VL patients infected with Leishmania donovani. We found little effect on parasite growth or parasite-specific IFNγ production. However, this treatment reversed the improved anti-parasitic immunity achieved by IL-10 signaling blockade. Further investigations using an experimental VL model caused by infection of C57BL/6 mice with L. donovani revealed that this negative effect was prominent in the liver, dependent on parasite burden and associated with an accumulation of Th1 cells expressing high levels of KLRG-1. Nevertheless, combined anti-IL-10 and anti-GITR mAb treatment could improve anti-parasitic immunity when used with sub-optimal doses of anti-parasitic drug. However, additional studies with VL patient samples indicated that targeting GITR had no overall benefit over IL-10 signaling blockade alone at improving anti-parasitic immune responses, even with drug treatment cover. These findings identify several important factors that influence the effectiveness of immune modulation, including parasite burden, target tissue and the use of anti-parasitic drug. Critically, these results also highlight potential negative effects of

  10. Visceral leishmaniasis treatment outcome and its determinants in northwest Ethiopia.

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    Welay, Getachew Mebrahtu; Alene, Kefyalew Addis; Dachew, Berihun Assefa

    2016-01-01

    Poor treatment outcomes of visceral leishmaniasis (VL) are responsible for the high mortality rate of this condition in resource-limited settings such as Ethiopia. This study aimed to identify the proportion of poor VL treatment outcomes in northwest Ethiopia and to evaluate the determinants associated with poor outcomes. A hospital-based retrospective study was conducted among 595 VL patients who were admitted to Kahsay Abera Hospital in northwest Ethiopia from October 2010 to April 2013. Data were entered into Epi Info version 7.0 and exported to SPSS version 20 for analysis. Bivariate and multivariate logistic regression models were fitted to identify the determinants of VL treatment outcomes. Adjusted odds ratio (aORs) with 95% confidence intervals (CIs) were used, and p -values <0.05 were considered to indicate statistical significance. The proportion of poor treatment outcomes was 23.7%. Late diagnosis (≥29 days) (aOR, 4.34; 95% CI, 2.22 to 8.46), severe illness at admission (inability to walk) (aOR, 1.63; 95% CI, 1.06 to 2.40) and coinfection with VL and human immunodeficiency virus (HIV) (aOR, 2.72; 95% CI, 1.40 to 5.20) were found to be determinants of poor VL treatment outcomes. Poor treatment outcomes, such as death, treatment failure, and non-adherence, were found to be common. Special attention must be paid to severely ill and VL/HIV-coinfected patients. To improve VL treatment outcomes, the early diagnosis and treatment of VL patients is recommended.

  11. Visceral Inflammation and Immune Activation Stress the Brain

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    Holzer, Peter; Farzi, Aitak; Hassan, Ahmed M.; Zenz, Geraldine; Jačan, Angela; Reichmann, Florian

    2017-01-01

    Stress refers to a dynamic process in which the homeostasis of an organism is challenged, the outcome depending on the type, severity, and duration of stressors involved, the stress responses triggered, and the stress resilience of the organism. Importantly, the relationship between stress and the immune system is bidirectional, as not only stressors have an impact on immune function, but alterations in immune function themselves can elicit stress responses. Such bidirectional interactions have been prominently identified to occur in the gastrointestinal tract in which there is a close cross-talk between the gut microbiota and the local immune system, governed by the permeability of the intestinal mucosa. External stressors disturb the homeostasis between microbiota and gut, these disturbances being signaled to the brain via multiple communication pathways constituting the gut–brain axis, ultimately eliciting stress responses and perturbations of brain function. In view of these relationships, the present article sets out to highlight some of the interactions between peripheral immune activation, especially in the visceral system, and brain function, behavior, and stress coping. These issues are exemplified by the way through which the intestinal microbiota as well as microbe-associated molecular patterns including lipopolysaccharide communicate with the immune system and brain, and the mechanisms whereby overt inflammation in the GI tract impacts on emotional-affective behavior, pain sensitivity, and stress coping. The interactions between the peripheral immune system and the brain take place along the gut–brain axis, the major communication pathways of which comprise microbial metabolites, gut hormones, immune mediators, and sensory neurons. Through these signaling systems, several transmitter and neuropeptide systems within the brain are altered under conditions of peripheral immune stress, enabling adaptive processes related to stress coping and resilience

  12. Cost of Pediatric Visceral Leishmaniasis Care in Morocco.

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    Nabil Tachfouti

    Full Text Available Visceral leishmaniasis (VL is a neglected parasitic disease that is fatal if left untreated. VL is endemic in Morocco and other countries in North Africa were it mainly affects children from rural areas. In Morocco, the direct observation of Leishmania parasites in bone marrow aspirates and serological tests are used to diagnose VL. Glucantime is the first line of treatment. The objective of this study was to report the costs associated to standard clinical management of pediatric VL from the provider perspective in Morocco. As a secondary objective we described the current clinical practices and the epidemiological characteristics of pediatric VL patients.From March to June 2014 we conducted a survey in eight hospitals treating pediatric VL patients in Morocco. A pro-forma was used to collect demographic, clinical and management data from medical records. We specifically collected data on VL diagnosis and treatment. We also estimated the days of hospitalization and the time to start VL treatment. Costs were estimated by multiplying the use of resources in terms of number of days in hospital, tests performed and drugs provided by the official prices. For patients receiving part of their treatment at Primary Health Centers (PHC we estimated the cost of administering the Glucantime as outpatient. We calculated the median cost per VL patient. We also estimated the cost of managing a VL case when different treatment strategies were applied: inpatient and outpatient.We obtained data from 127 VL patients. The median total cost per pediatric VL case in Morocco is 520 US$. The cost in hospitals applying an outpatient strategy is significantly lower (307 US$ than hospitals keeping the patients for the whole treatment (636 US$. However the outpatient strategy is not yet recommended as VL treatment for children in the Moroccan guidelines. VL diagnosis and treatment regimens should be standardized following the current guidelines in Morocco.

  13. Visceral leishmaniasis in the Indian subcontinent: modelling epidemiology and control.

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    Anette Stauch

    2011-11-01

    Full Text Available In the Indian subcontinent, about 200 million people are at risk of developing visceral leishmaniasis (VL. In 2005, the governments of India, Nepal and Bangladesh started the first regional VL elimination program with the aim to reduce the annual incidence to less than 1 per 10,000 by 2015. A mathematical model was developed to support this elimination program with basic quantifications of transmission, disease and intervention parameters. This model was used to predict the effects of different intervention strategies.Parameters on the natural history of Leishmania infection were estimated based on a literature review and expert opinion or drawn from a community intervention trial (the KALANET project. The transmission dynamic of Leishmania donovani is rather slow, mainly due to its long incubation period and the potentially long persistence of parasites in infected humans. Cellular immunity as measured by the Leishmanin skin test (LST lasts on average for roughly one year, and re-infection occurs in intervals of about two years, with variation not specified. The model suggests that transmission of L. donovani is predominantly maintained by asymptomatically infected hosts. Only patients with symptomatic disease were eligible for treatment; thus, in contrast to vector control, the treatment of cases had almost no effect on the overall intensity of transmission.Treatment of Kala-azar is necessary on the level of the individual patient but may have little effect on transmission of parasites. In contrast, vector control or exposure prophylaxis has the potential to efficiently reduce transmission of parasites. Based on these findings, control of VL should pay more attention to vector-related interventions. Cases of PKDL may appear after years and may initiate a new outbreak of disease; interventions should therefore be long enough, combined with an active case detection and include effective treatment.

  14. Toll-Like Receptor 4 in Paraventricular Nucleus Mediates Visceral Hypersensitivity Induced by Maternal Separation

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    Hui-Li Tang

    2017-05-01

    Full Text Available Neonatal maternal separation (MS is a major early life stress that increases the risk of emotional disorders, visceral pain perception and other brain dysfunction. Elevation of toll-like receptor 4 (TLR4 signaling in the paraventricular nucleus (PVN precipitates early life colorectal distension (CRD-induced visceral hypersensitivity and pain in adulthood. The present study aimed to investigate the role of TLR4 signaling in the pathogenesis of postnatal MS-induced visceral hypersensitivity and pain during adulthood. The TLR4 gene was selectively knocked out in C57BL/10ScSn mice (Tlr4-/-. MS was developed by housing the offspring alone for 6 h daily from postnatal day 2 to day 15. Visceral hypersensitivity and pain were assessed in adulthood. Tlr4+/+, but not Tlr4-/-, mice that had experienced neonatal MS showed chronic visceral hypersensitivity and pain. TLR4 immunoreactivity was observed predominately in microglia in the PVN, and MS was associated with an increase in the expression of protein and/or mRNA levels of TLR4, corticotropin-releasing factor (CRF, CRF receptor 1 (CRFR1, tumor necrosis factor-α, and interleukin-1β in Tlr4+/+ mice. These alterations were not observed in Tlr4-/- mice. Local administration of lipopolysaccharide, a TLR4 agonist, into the lateral cerebral ventricle elicited visceral hypersensitivity and TLR4 mRNA expression in the PVN, which could be prevented by NBI-35965, an antagonist to CRFR1. The present results indicate that neonatal MS induces a sensitization and upregulation of microglial TLR4 signaling activity, which facilitates the neighboring CRF neuronal activity and, eventually, precipitates visceral hypersensitivity in adulthood.Highlights(1Neonatal MS does not induce chronic visceral hypersensitivity and pain in Tlr4-/- mice.(2Neonatal MS increases the expression of TLR4 mRNA, CRF protein and mRNA, CRFR1 protein, TNF-α protein, and IL-1β protein in Tlr4+/+ mice.(3TLR4 agonist LPS (i.c.v. elicits visceral

  15. Canine Visceral Leishmaniasis in Boyer Ahmad District, Kohgiluyeh & Boyer Ahmad Province, Southwest of Iran

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    V Barati

    2012-12-01

    Full Text Available Background: Mediterranean type of visceral leishmaniasis (VL is present in different parts of Iran. Several studies have identified dogs as the main reservoirs of the VL caused by Leishmania infantum in Iran and other Mediterranean regions. This study aimed to determine the seroprevalence of canine visceral leishmaniasis as animal reservoir host for human visceral leishmaniasis in Boyer Ahmad dis­trict in southwest of Iran.Methods: A seroepidemiological study was carried out to determine the seroprevalence of canine visceral leishmaniasis (CVL among ownership dogs by using direct agglutination test (DAT in 23 of 182 villages of Boyer Ahmad district, during August 2009 to August 2010. One hundred and seventy serum samples from ownership dogs were selected by multi-stage cluster sampling in villages of Boyer Ahmad district. All samples were tested by DAT and anti-Leishmania antibodies titers at ≥ 1:320 was considered as positive.Results: Of the 170 serum samples, 10% were positive by DAT at titers of 1:320 and higher. No statistical significant difference was found between male (10.7% and female (8.3% seroprevalence. The highest seroprevalence rate (15.1% was observed among the ownership dogs of four to seven years age. Altogether, seventeen (25.4% of the seropositive dogs had clinical signs and symptoms.Conclusion: It seems that Boyer Ahmad district is an endemic area for canine visceral leishmaniasis in Iran.

  16. Visceral hypersensitive rats share common dysbiosis features with irritable bowel syndrome patients.

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    Zhou, Xiao-Yan; Li, Ming; Li, Xia; Long, Xin; Zuo, Xiu-Li; Hou, Xiao-Hua; Cong, Ying-Zi; Li, Yan-Qing

    2016-06-14

    To evaluate gut microbial dysbiosis in two visceral hypersensitive models in comparison with irritable bowel syndrome (IBS) patients and to explore the extent to which these models capture the dysbiosis of IBS patients. Visceral hypersensitivity was developed using the maternal separation (MS) rat model and post-inflammatory rat model. The visceral sensitivity of the model groups and control group was evaluated using the abdominal withdraw reflex score and electromyography in response to graded colorectal distention. The 16S ribosomal RNA gene from fecal samples was pyrosequenced and analyzed. The correlation between dysbiosis in the microbiota and visceral hypersensitivity was calculated. Positive findings were compared to sequencing data from a published human IBS cohort. Dysbiosis triggered by neonatal maternal separation was lasting but not static. Both MS and post-inflammatory rat fecal microbiota deviated from that of the control rats to an extent that was larger than the co-housing effect. Two short chain fatty acid producing genera, Fusobacterium and Clostridium XI, were shared by the human IBS cohort and by the maternal separation rats and post-inflammatory rats, respectively, to different extents. Fusobacterium was significantly increased in the MS group, and its abundance positively correlated with the degree of visceral hypersensitivity. Porphyromonadaceae was a protective biomarker for both the rat control group and healthy human controls. The dysbiosis MS rat model and the post-inflammatory rat model captured some of the dysbiosis features of IBS patients. Fusobacterium, Clostridium XI and Porphyromonadaceae were identified as targets for future mechanistic research.

  17. Role of nesfatin-1 in a rat model of visceral hypersensitivity.

    Science.gov (United States)

    Jia, Fang-Yuan; Li, Xue-Liang; Li, Tian-Nv; Wu, Jing; Xie, Bi-Yun; Lin, Lin

    2013-06-14

    To explore the role of nesfatin-1 on irritable bowel syndrome (IBS)-like visceral hypersensitivity. The animal model of IBS-like visceral hypersensitivity was induced by intracolonic infusion of 0.5% acetic acid (AA) in saline once daily from postnatal days 8-21. Experiments were performed when rats became adults. The visceral sensitivity of rats was evaluated by abdominal withdrawal reflex (AWR) and electromyographic (EMG) activity of the external oblique muscle to graded colorectal distension. The content of nesfatin-1 in serum was determined using enzyme-linked immunosorbent assay. After implantation of an intracerebroventricular (ICV) cannula and two electrodes into the external oblique muscle, model rats were randomly divided into four groups. Animals then received ICV injection of 8 μg of anti-nesfatin-1/nucleobindin-2 (NUCB2), 50 μg of α-helical corticotropin releasing factor (CRF) 9-41 (non-selective CRF receptor antagonist), 50 μg of NBI-27914 (selective CRF1 receptor antagonist) or 5 μL of vehicle. After 1 h of ICV administration, visceral sensitivity of each group was measured again, and comparisons between groups were made. Rats treated with AA showed higher mean AWR scores and EMG activity at all distension pressures compared with controls (P IBS-like visceral hypersensitivity, which may be implicated in brain CRF/CRF1 signaling pathways.

  18. The monoamine reuptake inhibitor milnacipran does not affect nociception to acute visceral distension in rats.

    Science.gov (United States)

    Shin, Sang-Wook; Eisenach, James C; Rao, Srinias G; Tong, Chuanyao

    2004-05-01

    The role of antidepressants in the treatment of visceral pain has not been extensively examined. Milnacipran, an antidepressant that inhibits monoamine reuptake, is widely used in the treatment of depression and fibromyalgia. In this study, we sought to determine the activity of milnacipran against acute visceral nociception. Female virgin rats were studied 7 days after bilateral ovariectomy. For uterine cervical distension (UCD), two metal rods were inserted into the cervical osses under general anesthesia for manual distension. Colorectal distension (CRD) was performed by insertion of a balloon catheter into the descending colon and rectum, followed by manual inflation. Two electrodes were inserted into the rectus abdominus muscle for recording UCD- or CRD-induced reflex contraction, which was quantified by electromyography (EMG). A dose response for milnacipran, administered intrathecally or i.v., was obtained for UCD and CRD stimulation. Milnacipran failed to inhibit the UCD-induced EMG response, whether administered i.v. or intrathecally. Similarly, i.v. milnacipran, administered either acutely or chronically, failed to inhibit the CRD-induced EMG response. CRD and UCD are well established animal models for the study of acute visceral pain. Milnacipran, although it provides some unique advantages compared with other antidepressants, is unlikely to produce analgesia after acute administration in the setting of acute visceral pain. Neither intrathecal nor i.v. milnacipran, a monoamine reuptake inhibitor, inhibits an acute visceral pain response induced by colorectal or uterine cervical distension.

  19. Rectal visceral sensitivity in women with irritable bowel syndrome without psychiatric comorbidity compared with healthy volunteers.

    Science.gov (United States)

    Spetalen, Signe; Sandvik, Leiv; Blomhoff, Svein; Jacobsen, Morten B

    2009-01-01

    Psychiatric comorbidity and visceral hypersensitivity are common in patients with irritable bowel syndrome (IBS), but little is known about visceral sensitivity in IBS patients without psychiatric disorders. We wanted to examine rectal visceral sensitivity in IBS patients without comorbid psychiatric disorders, IBS patients with phobic anxiety and healthy volunteers. A total of thirty-eight female, non-constipated IBS patients without psychiatric disorders and eleven female IBS patients with phobic anxiety were compared to nine healthy women using a barostat double random staircase method. The non-psychiatric patients were divided into those with diarrhoea predominant symptoms and those with alternating stool habits. The IBS patients without psychiatric disorders had normal visceral pressure thresholds. However, in the diarrhoea predominant subgroup, the volume discomfort threshold was reduced while it was unchanged in those with alternating stool habits. The phobic IBS patients had similar thresholds to the healthy volunteers. The rectal tone was increased in the non-psychiatric IBS patients with diarrhoea predominant symptoms and in the IBS patients with phobic anxiety. Non-constipated IBS patients without psychiatric disorders had increased visceral sensitivity regarding volume thresholds but normal pressure thresholds. Our study suggests that the lowered volume threshold was due to increased rectal tone.

  20. Mechanisms Underlying the Analgesic Effect of Moxibustion on Visceral Pain in Irritable Bowel Syndrome: A Review

    Directory of Open Access Journals (Sweden)

    Renjia Huang

    2014-01-01

    Full Text Available Irritable bowel syndrome (IBS is a functional bowel disorder that causes recurrent abdominal (visceral pain. Epidemiological data show that the incidence rate of IBS is as high as 25%. Most of the medications may lead to tolerance, addiction and toxic side effects. Moxibustion is an important component of traditional Chinese medicine and has been used to treat IBS-like abdominal pain for several thousand years in China. As a mild treatment, moxibustion has been widely applied in clinical treatment of visceral pain in IBS. In recent years, it has played an irreplaceable role in alternative medicine. Extensive clinical studies have demonstrated that moxibustion for treatment of visceral pain is simple, convenient, and inexpensive, and it is being accepted by an increasing number of patients. There have not been many studies investigating the analgesic mechanisms of moxibustion. Studies exploring the analgesic mechanisms have mainly focused on visceral hypersensitivity, brain-gut axis neuroendocrine system, and immune system. This paper reviews the latest developments in moxibustion use for treatment of visceral pain in IBS from these perspectives. It also evaluates potential problems in relevant studies on the mechanisms of moxibustion therapy to promote the application of moxibustion in the treatment of IBS.

  1. Stress and the Microbiota-Gut-Brain Axis in Visceral Pain: Relevance to Irritable Bowel Syndrome.

    Science.gov (United States)

    Moloney, Rachel D; Johnson, Anthony C; O'Mahony, Siobhain M; Dinan, Timothy G; Greenwood-Van Meerveld, Beverley; Cryan, John F

    2016-02-01

    Visceral pain is a global term used to describe pain originating from the internal organs of the body, which affects a significant proportion of the population and is a common feature of functional gastrointestinal disorders (FGIDs) such as irritable bowel syndrome (IBS). While IBS is multifactorial, with no single etiology to completely explain the disorder, many patients also experience comorbid behavioral disorders, such as anxiety or depression; thus, IBS is described as a disorder of the gut-brain axis. Stress is implicated in the development and exacerbation of visceral pain disorders. Chronic stress can modify central pain circuitry, as well as change motility and permeability throughout the gastrointestinal (GI) tract. More recently, the role of the gut microbiota in the bidirectional communication along the gut-brain axis, and subsequent changes in behavior, has emerged. Thus, stress and the gut microbiota can interact through complementary or opposing factors to influence visceral nociceptive behaviors. This review will highlight the evidence by which stress and the gut microbiota interact in the regulation of visceral nociception. We will focus on the influence of stress on the microbiota and the mechanisms by which microbiota can affect the stress response and behavioral outcomes with an emphasis on visceral pain. © 2015 John Wiley & Sons Ltd.

  2. Canine ehrlichiosis: clinical, hematological, serological and molecular aspects Erliquiose canina: aspectos clínicos, hematológicos, sorológicos e moleculares

    Directory of Open Access Journals (Sweden)

    Andréa Cristina Higa Nakaghi

    2008-06-01

    écnicas, concluiu-se que a sorologia e a nPCR são testes adequados para a confirmação do diagnóstico da erliquiose canina. Entretanto, os resultados destas técnicas devem sempre ser complementares ao exame clínico e hematológico. A sorologia tem um importante papel nas fases subclínica e crônica da doença, por isso recomenda-se a nPCR para o diagnóstico na fase aguda e, especialmente, para a identificação da espécie de erliquia envolvida.

  3. Retrospective study (1998-2001 on canine ehrlichiosis in Belo Horizonte, MG, Brazil Estudo retrospectivo (1998 a 2001 da erliquiose canina em Belo Horizonte

    Directory of Open Access Journals (Sweden)

    S.M. Moreira

    2003-04-01

    . O número de diagnósticos de erliquiose aumentou consideravelmente a partir de 2000, sendo que 24,4% dos casos ocorreram em animais entre 13 e 24 meses de idade na cidade de Belo Horizonte e áreas peri-urbanas, verificando-se maior risco de infecção em animais que residiam em casas. Os sintomas mais freqüentes foram febre, anorexia, apatia, dor abdominal, linfadenopatia e dispnéia. Quanto às alterações hematológicas, 70,3% apresentaram anemia, 50% trombocitopenia e 30% leucopenia e a maioria das infecções por E. canis foi visualizada em monócitos. Os resultados apontam para a necessidade de maior atenção por parte dos clínicos e técnicos para a erliquiose canina uma vez que 35,9% dos cães atendidos com suspeita de hemoparasitoses apresentavam infecções por E. canis ou E. platys.

  4. Denervação capsular percutânea no tratamento da displasia coxofemoral canina Percutaneous hip denervation for the treatment of canine hip dysplasia

    Directory of Open Access Journals (Sweden)

    André Luis Selmi

    2009-04-01

    Full Text Available A displasia coxofemoral canina (DCF é uma afecção ortopédica freqüente, comumente dolorosa, decorrente de instabilidade e que leva à doença articular degenerativa. Várias formas de tratamento cirúrgico são descritas, entre elas a denervação capsular a céu aberto. Neste trabalho, é descrita a denervação capsular percutânea e seus efeitos em 92 cães com sensibilidade dolorosa na articulação do quadril atribuída à DCF. Foram avaliados os seguintes aspectos: o grau de claudicação (CLAUD, a dor por escala analógica visual, a perimetria da coxa (PC e os graus máximos de extensão (GME e flexão (GMF passiva do quadril imediatamente antes da cirurgia e aos sete, 15, 30, 180 e 360 dias de pós-operatório (p.o.. Os animais foram pré-medicados e submetidos à anestesia geral inalatória e, posteriormente, a borda acetabular crânio-dorsal foi denervada por meio da introdução percutânea de um pino de Steinman com aproximadamente 4,5mm de diâmetro. A CLAUD e a dor foram analisadas por meio da ANOVA e do teste de Friedman. A PC, a GME e a GMF foram analisadas por meio da ANOVA e do teste de Tukey. Foi observada melhora significativa na CLAUD e dor a partir dos 15 dias de p.o. A GME apresentou aumento significativo já aos 30 dias de p.o., enquanto que a PC apresentou aumento significativo após os 60 dias de p.o. Conclui-se que a denervação capsular percutânea é alternativa cirúrgica eficaz em restabelecer a movimentação do quadril displásico e promove melhora significativa da dor e claudicação.Canine hip dysplasia is a common painfull orthopedic disorder, resulting from articular instability and causing degenerative joint disease. Several treatment options are available, including capsular denervation. This study describes the results of percutaneous capsular denervation in 92 dogs diagnosed with hip dysplasia. Lameness (LAM and pain on palpation (PN were determined using a visual analogue scale (VAS, muscle girth

  5. Predictive performance of the visceral adiposity index for a visceral adiposity-related risk: Type 2 Diabetes

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    Azizi Fereidoun

    2011-05-01

    Full Text Available Abstract Background Visceral adiposity index (VAI has recently been developed based on waist circumference, body mass index (BMI, triglycerides (TGs, and high-density lipoprotein cholesterol (HDL-C. We examined predictive performances for incident diabetes of the VAI per se and as compared to the metabolic syndrome (MetS and waist-to-height-ratio (WHtR. Methods Participants free of diabetes at baseline with at least one follow-up examination (5,964 were included for the current study. Weibull regression models were developed for interval-censored survival data. Absolute and relative integrated discriminatory improvement index (IDI and cut-point-based and cut-point-free net reclassification improvement index (NRI were used as measures of predictive ability for incident diabetes added by VAI, as compared to the MetS and WHtR. Results The annual incidence rate of diabetes was 0.85 per 1000 person. Mean VAI was 3.06 (95%CIs 2.99-3.13. Diabetes risk factors levels increased in stepwise fashion across VAI quintiles. Risk gradient between the highest and lowest quintile of VAI was 4.5 (95%CIs 3.0-6.9. VAI significantly improved predictive ability of the MetS. The relative IDI and cut-point free NRI for predictive ability added to MetS by VAI were 30.3% (95%CIs 18.8-41.8% and 30.7% (95%CIs 20.8-40.7%, respectively. WHtR, outperformed VAI with cut-point-free NRI of 24.6% (95%CIs 14.1-35.2%. Conclusions In conclusion, although VAI could be a prognostic tool for incident diabetes events, gathering information on its components (WC, BMI, TGs, and HDL-C is unlikely to improve the prediction ability beyond what could be achieved by the simply assessable and commonly available information on WHtR.

  6. [The significance of stress intensity for the emotional and visceral reactivity, especially for blood pressure regulation].

    Science.gov (United States)

    Hecht, K; Hai, N V; Moritz, V; Hecht, T

    1976-01-01

    The influence of light, medium, and heavy chronic stress upon cerebro-visceral functions following 3 and 5 weeks of daily stress exposure was examined. The following results were obtained: Light stress produced no changes of cerebro-visceral functions. Medium stress restricted the learning and memory capacity, increased systolic blood pressure, fasting blood sugar and adrenal weights. The B-cells of the pancreatic islets showed degranulations of varying intensity. Heavy stress diminished the learning and memory capacity, increased adrenal weights and led to hypergranulation of the pancreatic B-cells. Blood sugar and blood pressure values fall within physiological limits. Load tests, however, revealed in these animals symptoms of premorbid states. Since throughout the period of observation, light stress stablized the regulation processes, medium stress induced early stages of pathological processes, and heavy stress caused premorbid states, no linearity could be established between the load intensity and the changes of cerebro-visceral functions.

  7. Translational pain research: evaluating analgesic effect in experimental visceral pain models

    DEFF Research Database (Denmark)

    Olesen, Anne Estrup; Andresen, Trine; Christrup, Lona Louring

    2009-01-01

    facilitate minimizing the gap between knowledge gained in animal and human clinical studies. Combining experimental pain studies and pharmacokinetic studies can improve understanding of the pharmacokinetic-pharmacodynamic relationship of analgesics and, thus, provide valuable insight into optimal clinical...... analgesics in detail. In combination with pharmacokinetic studies and objective assessment such as electroencephalography, new information regarding a given drug substance and its effects can be obtained. Results from experimental human visceral pain research can bridge the gap in knowledge between animal......Deep visceral pain is frequent and presents major challenges in pain management, since its pathophysiology is still poorly understood. One way to optimize treatment of visceral pain is to improve knowledge of the mechanisms behind the pain and the mode of action of analgesic substances. This can...

  8. Predicting pelvic visceral hypersensitivity from the discomfort of Lugol' test during colposcopy.

    Science.gov (United States)

    Indraccolo, U; Scutiero, G; Greco, P

    2016-07-01

    Assessing the pelvic visceral hypersensitivity after Lugol' staining during colposcopy. On an unselected sample of 91 patients, pelvic discomfort and discomfort during menstruation, sexual intercourse, defecation, urination were assessed (numeric rating score from 0 to 10) before colposcopy. Few minutes after Lugol' staining, it was assessed the discomforting perception provoked by iodine by using the same numeric rating scale. All those scores were increased by 1 to allow regression models (linear, logarithmic, inverse, cubic, quadratic, exponential). The only significant models were the linear, logarithmic and inverse ones for pelvic discomfort overall score and the linear one for sexual intercourse score. A discomfort score of 4.5 or less after Lugol' staining should be a normal reaction to iodine nociception in the vagina. Lugol' staining during colposcopy causes discomfort related with visceral nociception. The rise in numeric rating score after colposcopy can be used for screening pelvic visceral hypersensitivity.

  9. Visceral adipose tissue as a source of inflammation and promoter of atherosclerosis.

    Science.gov (United States)

    Alexopoulos, Nikolaos; Katritsis, Demosthenes; Raggi, Paolo

    2014-03-01

    The current epidemic of obesity with the associated increasing incidence of insulin resistance, diabetes mellitus and atherosclerosis affecting a large proportion of the North American and Western populations, has generated a strong interest in the potential role of visceral adipose tissue in the development of atherosclerosis and its complications. The intra-abdominal and epicardial space are two compartments that contain visceral adipose tissue with a similar embryological origin. These visceral fats are highly inflamed in obese patients, patients with the metabolic syndrome and in those with established coronary artery disease; additionally they are capable of secreting large quantities of pro-inflammatory cytokines and free fatty acids. There is accumulating evidence to support a direct involvement of these regional adipose tissue deposits in the development of atherosclerosis and its complicating events, as will be reviewed in this article. Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

  10. Stromal Cells Derived from Visceral and Obese Adipose Tissue Promote Growth of Ovarian Cancers.

    Directory of Open Access Journals (Sweden)

    Yan Zhang

    Full Text Available Obesity, and in particular visceral obesity, has been associated with an increased risk of developing cancers as well as higher rates of mortality following diagnosis. The impact of obesity on adipose-derived stromal cells (ASC, which contribute to the formation of tumor stroma, is unknown. Here we hypothesized that visceral source and diet-induced obesity (DIO changes the ASC phenotype, contributing to the tumor promoting effects of obesity. We found that ASC isolated from subcutaneous (SC-ASC and visceral (V-ASC white adipose tissue(WAT of lean(Le and obese(Ob mice exhibited similar mesenchymal cell surface markers expression, and had comparable effects on ovarian cancer cell proliferation and migration. Obese and visceral derived ASC proliferated slower and exhibited impaired differentiation into adipocytes and osteocytes in vitro as compared to ASC derived from subcutaneous WAT of lean mice. Intraperitoneal co-injection of ovarian cancer cells with obese or visceral derived ASC, but not lean SC-ASC, increased growth of intraperitoneal ID8 tumors as compared to controls. Obese and V-ASC increased stromal infiltration of inflammatory cells, including CD3+ T cells and F4/80+ macrophages. Obese and visceral derived ASC, but not lean SC-ASC, increased expression of chemotactic factors IL-6, MIP-2, and MCP-1 when cultured with tumor cells. Overall, these results demonstrate that obese and V-ASC have a unique phenotype, with more limited proliferation and differentiation capacity but enhanced expression of chemotactic factors in response to malignant cells which support infiltration of inflammatory cells and support tumor growth and dissemination.

  11. Runx1-deficient afferents impair visceral nociception, exacerbating dextran sodium sulfate-induced colitis.

    Science.gov (United States)

    Hung, Shih-Ping; Sheu, Ming-Jen; Ma, Ming Chieh; Hu, Jui-Ting; Sun, Ya-Yun; Lee, Chin-Cheng; Chung, Yuan-Chiang; Tsai, Yi-Ju; Wang, Jing-Yuan; Chen, Chih-Li

    2014-01-01

    Colitis is a group of inflammatory and auto-immune disorders that affect the tissue lining of the gastrointestinal (GI) system. Studies of chemically-induced animal models of colitis have indicated that nociceptive afferents or neuropeptides have differing effects on GI inflammation. However, the molecular mechanisms involved in visceral pain and the role of visceral sensory afferents involved in the modulation of colitis remains unclear. A previous study demonstrated that Runx1, a Runt domain transcription factor, is restricted to nociceptors. In these neurons, Runx1 regulates the expression of numerous ion channels and receptors, controlling the lamina-specific innervation patterns of nociceptive afferents in the spinal cord. Moreover, mice that lack Runx1 exhibit specific defects in thermal and neuropathic pain. To examine the function of Runx1 in visceral nociception, we employed double-transgenic mice (WntCre: Runx1(F/F)), in which the expression of Runx1 was specifically disrupted in the sensory neurons. To determine the role of Runx1 in visceral pain sensation, the WntCre: Runx1(F/F) mice and their control littermates (Runx1(F/F)) were treated using dextran sodium sulfate (DSS) to induce colitis. The results indicated that disrupted Runx1 in the sensory afferents resulted in: (1) impairment of the visceral pain sensation in murine DSS-induced colitis; (2) exacerbating the phenotypes in murine DSS-induced colitis; (3) a differential effect on the production of pro- and anti-inflammatory cytokines in the colon tissues isolated from mice treated using DSS and 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced colitis; and (4) alteration of the distribution of lymphocytes and mast cells in mucosa. These results show that the function of Runx1 in sensory afferents is vital for modulating visceral pain and the neuro-immune axis. Copyright © 2013 Elsevier Inc. All rights reserved.

  12. Risk factors, representations and practices associated with emerging urban human visceral leishmaniasis in Posadas, Argentina.

    Science.gov (United States)

    López, Karen; Tartaglino, Lilian Catalina; Steinhorst, Ingrid Iris; Santini, María Soledad; Salomon, Oscar Daniel

    2016-02-23

    Visceral leishmaniasis is an often overlooked disease with high lethality rates about which there is need of additional local studies to inform the design of effective control strategies. The urbanization of its transmission has already been verified in America, with domestic dogs being the primary reservoirs and vectors of the disease. Socio-economic conditions, demographics and practices of domestic groups typically present in urban settings may play a specific role in the transmission of the infection, which is still poorly understood.  To analyze the sociodemographic characteristics, risk factors and overall practices concerning prevention and coping strategies of visceral leishmaniasis, in both human beings and canines.  This study utilized a cross-sectional case-control design. Cases were defined as a domestic group where the Public Health Ministry had at least one record of a member with human visceral leishmaniasis. Control cases were defined as a domestic group without a clinical record of the disease. The populations were characterized demographically and socially using primary information sources. Measures of household quality and a ranking of knowledge and attitudes towards visceral leishmaniasis were constructed, and practices associated with the presence, and the risk for canine visceral leishmaniasis were described.  Low household quality (p≤0.001), a member of the domestic group out of the household after 6:00 pm (OR=4.4; 95% CI: 1.69-12.18), the uncontrolled racial breeding of dogs (OR=15.7; 95% CI: 3.91-63.2), and the presence of infected dogs infected in the household (OR=120.3; 95% CI: 18.51-728.3) were variables positively associated with the risk of infection.  We observed certain social risk factors, primarily low household quality and overcrowding, associated with structural poverty that could increase human-vector contact probability. The most important risk factor for human visceral leishmaniasis was the possession of infected dogs

  13. Stromal Cells Derived from Visceral and Obese Adipose Tissue Promote Growth of Ovarian Cancers.

    Science.gov (United States)

    Zhang, Yan; Nowicka, Aleksandra; Solley, Travis N; Wei, Caimiao; Parikh, Aaroh; Court, Laurence; Burks, Jared K; Andreeff, Michael; Woodward, Wendy A; Dadbin, Ali; Kolonin, Mikhail G; Lu, Karen H; Klopp, Ann H

    2015-01-01

    Obesity, and in particular visceral obesity, has been associated with an increased risk of developing cancers as well as higher rates of mortality following diagnosis. The impact of obesity on adipose-derived stromal cells (ASC), which contribute to the formation of tumor stroma, is unknown. Here we hypothesized that visceral source and diet-induced obesity (DIO) changes the ASC phenotype, contributing to the tumor promoting effects of obesity. We found that ASC isolated from subcutaneous (SC-ASC) and visceral (V-ASC) white adipose tissue(WAT) of lean(Le) and obese(Ob) mice exhibited similar mesenchymal cell surface markers expression, and had comparable effects on ovarian cancer cell proliferation and migration. Obese and visceral derived ASC proliferated slower and exhibited impaired differentiation into adipocytes and osteocytes in vitro as compared to ASC derived from subcutaneous WAT of lean mice. Intraperitoneal co-injection of ovarian cancer cells with obese or visceral derived ASC, but not lean SC-ASC, increased growth of intraperitoneal ID8 tumors as compared to controls. Obese and V-ASC increased stromal infiltration of inflammatory cells, including CD3+ T ce