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Sample records for viia lepane marina

  1. Perilaku Ibu dalam Pemanfaatan Pelayanan Kesehatan Gigi dan Mulut pada Anak Usia Sekolah di SDN 054936 Wonorejo Kecamatan Sei Lepan Kabupaten Langkat Tahun 2013

    OpenAIRE

    Ningsih, Vera

    2015-01-01

    School age children are susceptible to oral health problem, SDN 054936 Sub Wonorejo Sei Lepan Langkat District located closest to The Old Village district health center services Sei Lepan. Visits to the school age children oral poly very small and there are many children who have cavities. This study aims to determine the mother’s behavior regarding the use of oral health services to school age children in Elemantary School district 054936 Sei Lepan Langkat district. This type of researc...

  2. Haemostatic effects of recombinant coagulation factor VIIa

    NARCIS (Netherlands)

    Lisman, Johannes Antonius

    2002-01-01

    Recombinant coagulation factor VIIa (rFVIIa) has recently become available for treatment of patients with inhibitor-complicated haemophilia. It has been postulated that rFVIIa could become a universal haemostatic agent. Case reports and small studies confirm efficacy and safety of rFVIIa in a

  3. Las hierbas marinas.

    OpenAIRE

    Baisre, J. A.

    2007-01-01

    Principales tipos y características distintivas de las hierbas marinas: hierba de tortuga marina Thalassia testudinum, hierba de manatí Syringodium filiforme y la hierba de bajío Halodule wrigti. Las mismas sirven como alimento a muchas especies marinas.

  4. Cargo binding activates myosin VIIA motor function in cells.

    Science.gov (United States)

    Sakai, Tsuyoshi; Umeki, Nobuhisa; Ikebe, Reiko; Ikebe, Mitsuo

    2011-04-26

    Myosin VIIA, thought to be involved in human auditory function, is a gene responsible for human Usher syndrome type 1B, which causes hearing and visual loss. Recent studies have suggested that it can move processively if it forms a dimer. Nevertheless, it exists as a monomer in vitro, unlike the well-known two-headed processive myosin Va. Here we studied the molecular mechanism, which is currently unknown, of activating myosin VIIA as a cargo-transporting motor. Human myosin VIIA was present throughout cytosol, but it moved to the tip of filopodia upon the formation of dimer induced by dimer-inducing reagent. The forced dimer of myosin VIIA translocated its cargo molecule, MyRip, to the tip of filopodia, whereas myosin VIIA without the forced dimer-forming module does not translocate to the filopodial tips. These results suggest that dimer formation of myosin VIIA is important for its cargo-transporting activity. On the other hand, myosin VIIA without the forced dimerization module became translocated to the filopodial tips in the presence of cargo complex, i.e., MyRip/Rab27a, and transported its cargo complex to the tip. Coexpression of MyRip promoted the association of myosin VIIA to vesicles and the dimer formation. These results suggest that association of myosin VIIA monomers with membrane via the MyRip/Rab27a complex facilitates the cargo-transporting activity of myosin VIIA, which is achieved by cluster formation on the membrane, where it possibly forms a dimer. Present findings support that MyRip, a cargo molecule, functions as an activator of myosin VIIA transporter function.

  5. Factor VIIa binding and internalization in hepatocytes

    DEFF Research Database (Denmark)

    Hjortoe, G; Sorensen, B B; Petersen, L C

    2005-01-01

    The liver is believed to be the primary clearance organ for coagulation proteases, including factor VIIa (FVIIa). However, at present, clearance mechanisms for FVIIa in liver are unknown. To obtain information on the FVIIa clearance mechanism, we investigated the binding and internalization...... of FVIIa in liver cells using a human hepatoma cell line (HEPG2), and primary rat and human hepatocytes as cell models. 125I-FVIIa bound to HEPG2 cells in a time- and dose-dependent manner. Anti-tissue factor antibodies reduced the binding by about 25%, whereas 50-fold molar excess of unlabeled FVIIa had...

  6. Cargo binding activates myosin VIIA motor function in cells

    OpenAIRE

    Sakai, Tsuyoshi; Umeki, Nobuhisa; Ikebe, Reiko; Ikebe, Mitsuo

    2011-01-01

    Myosin VIIA, thought to be involved in human auditory function, is a gene responsible for human Usher syndrome type 1B, which causes hearing and visual loss. Recent studies have suggested that it can move processively if it forms a dimer. Nevertheless, it exists as a monomer in vitro, unlike the well-known two-headed processive myosin Va. Here we studied the molecular mechanism, which is currently unknown, of activating myosin VIIA as a cargo-transporting motor. Human myosin VIIA was present ...

  7. Allosteric activation of coagulation factor VIIa visualized by hydrogen exchange

    DEFF Research Database (Denmark)

    Rand, Kasper Dyrberg; Jørgensen, Thomas; Olsen, Ole H

    2006-01-01

    Coagulation factor VIIa (FVIIa) is a serine protease that, after binding to tissue factor (TF), plays a pivotal role in the initiation of blood coagulation. We used hydrogen exchange monitored by mass spectrometry to visualize the details of FVIIa activation by comparing the exchange kinetics...

  8. Expression of myosin VIIA in the developing chick inner ear neurons.

    Science.gov (United States)

    Nguyen, Kristi; Hall, Amanda L; Jones, Jennifer M

    2015-01-01

    The auditory-vestibular ganglion (AVG) is formed by the division of otic placode-derived neuroblasts, which then differentiate into auditory and vestibular afferent neurons. The developmental mechanisms that regulate neuronal cell fate determination, axonal pathfinding and innervation of otic neurons are poorly understood. The present study characterized the expression of myosin VIIA, along with the neuronal markers, Islet1, NeuroD1 and TuJ1, in the developing avian ear, during Hamburger-Hamilton (HH) stages 16-40. At early stages, when neuroblasts are delaminating from the otic epithelium, myosin VIIA expression was not observed. Myosin VIIA was initially detected in a subset of neurons during the early phase of neuronal differentiation (HH stage 20). As the AVG segregates into the auditory and vestibular portions, myosin VIIA was restricted to a subset of vestibular neurons, but was not present in auditory neurons. Myosin VIIA expression in the vestibular ganglion was maintained through HH stage 33 and was downregulated by stage 36. Myosin VIIA was also observed in the migrating processes of vestibular afferents as they begin to innervate the otic epithelium HH stage 22/23. Notably, afferents targeting hair cells of the cristae were positive for myosin VIIA while afferents targeting the utricular and saccular maculae were negative (HH stage 26-28). Although previous studies have reported that myosin VIIA is restricted to sensory hair cells, our data shows that myosin VIIA is also expressed in neurons of the developing chick ear. Our study suggests a possible role for myosin VIIA in axonal migration/pathfinding and/or innervation of vestibular afferents. In addition, myosin VIIA could be used as an early marker for vestibular neurons during the development of the avian AVG. Copyright © 2015 Elsevier B.V. All rights reserved.

  9. Myosin VIIA, important for human auditory function, is necessary for Drosophila auditory organ development.

    Directory of Open Access Journals (Sweden)

    Sokol V Todi

    Full Text Available BACKGROUND: Myosin VIIA (MyoVIIA is an unconventional myosin necessary for vertebrate audition [1]-[5]. Human auditory transduction occurs in sensory hair cells with a staircase-like arrangement of apical protrusions called stereocilia. In these hair cells, MyoVIIA maintains stereocilia organization [6]. Severe mutations in the Drosophila MyoVIIA orthologue, crinkled (ck, are semi-lethal [7] and lead to deafness by disrupting antennal auditory organ (Johnston's Organ, JO organization [8]. ck/MyoVIIA mutations result in apical detachment of auditory transduction units (scolopidia from the cuticle that transmits antennal vibrations as mechanical stimuli to JO. PRINCIPAL FINDINGS: Using flies expressing GFP-tagged NompA, a protein required for auditory organ organization in Drosophila, we examined the role of ck/MyoVIIA in JO development and maintenance through confocal microscopy and extracellular electrophysiology. Here we show that ck/MyoVIIA is necessary early in the developing antenna for initial apical attachment of the scolopidia to the articulating joint. ck/MyoVIIA is also necessary to maintain scolopidial attachment throughout adulthood. Moreover, in the adult JO, ck/MyoVIIA genetically interacts with the non-muscle myosin II (through its regulatory light chain protein and the myosin binding subunit of myosin II phosphatase. Such genetic interactions have not previously been observed in scolopidia. These factors are therefore candidates for modulating MyoVIIA activity in vertebrates. CONCLUSIONS: Our findings indicate that MyoVIIA plays evolutionarily conserved roles in auditory organ development and maintenance in invertebrates and vertebrates, enhancing our understanding of auditory organ development and function, as well as providing significant clues for future research.

  10. A loop of coagulation factor VIIa influencing macromolecular substrate specificity

    DEFF Research Database (Denmark)

    Bjelke, Jais R; Persson, Egon; Rasmussen, Hanne B

    2006-01-01

    Coagulation factor VIIa (FVIIa) belongs to a family of proteases being part of the stepwise, self-amplifying blood coagulation cascade. To investigate the impact of the mutation Met(298{156})Lys in FVIIa, we replaced the Gly(283{140})-Met(298{156}) loop with the corresponding loop of factor Xa....../Met(298{156})Lys-FVIIa with almost the same activity and specificity profile. We conclude that a lysine residue in position 298{156} of FVIIa requires a hydrophilic environment to be fully accommodated. This position appears critical for substrate specificity among the proteases of the blood coagulation...

  11. Recombinant Factor VIIa-Mediated Activation of Prothrombin Complex Concentrates.

    Science.gov (United States)

    Sadeghi, Nasiredin; Iacobelli, Massimo; Vaziri, Behroz; Kahn, Daniel; Hoppensteadt, Debra; Guler, Nil; Fareed, Jawed

    2017-04-01

    Recombinant factor VIIa (rFVIIa) is used in the management of bleeding in patients with hemophilia. A generic biosimilar version of NovoSeven is also developed (AryoSeven). To compare the activation profile of NovoSeven and AryoSeven, 2 commercially available protein complex concentrates (PCCs) were used. Profilnine activated by RecombiPlasTin 2G resulted in conversions of prothrombin to prethrombin and thrombin at 5 to 30 minutes. However, addition of rFVIIa at final concentration range of 0.25 to 0.5 µg/mL to the same mixture resulted in total conversion of prothrombin to thrombin with a doublet at 36 kDa. Recombinant factor VIIa alone did not generate thrombin in native Beriplex, and the addition of rFVIIa to Beriplex failed to generate thrombin. Beriplex activated by RecombiPlasTin 2G resulted in complete conversion of prothrombin to thrombin. Both NovoSeven and AryoSeven exhibited similar activation profiles. These studies indicate that the activation of PCCs by both rFVIIa preparations results in comparable generation of thrombin.

  12. The use of recombinant factor VIIa in a pediatric septic shock patient with disseminated intravascular coagulation

    Directory of Open Access Journals (Sweden)

    Ronaldo Arkader

    2008-03-01

    Full Text Available This is a report on a pediatric patient with septic shock and disseminated intravascular coagulation, who developed life-threatening bleeding which was successfully treated with recombinant factor VIIa.

  13. Hindi tagandamine võib viia võimukoalitsiooni lagunemiseni Kohtla-Järvel / Erik Gamzejev

    Index Scriptorium Estoniae

    Gamzejev, Erik, 1967-

    2003-01-01

    Ilmunud ka: Severnoje Poberezhje 26. sept. lk. 1. Keskerakonna soov saada endale Kohtla-Järve linnapea ametikoht võib viia Keskerakonna ja endiste Res Publica liikmete vahel sõlmitud koalitsiooni lagunemiseni

  14. Tortugas marinas en aguas argentinas

    OpenAIRE

    Gonzalez Carman, V.; Mianzan, H.; Bruno, I.; Prosdocimi, L.; Albareda, D.; Campagna, C.

    2012-01-01

    Con la ayuda de los sensores remotos y los sistemas de posicionamiento geográfico, los científicos están descubriendo varias facetas sobre las vida de las tortugas marinas. Se describe el método para realizar el siguimiento satelital, sus migraciones, y se dan las características de las especies de tortugas marinas encontradas en aguas costeras de la Argentina. Este trabajo de divulgación científica hace hincapié en la importancia de su conservación, según la Unión Internacional para la Conse...

  15. Splenic embolization in a Jehovah's Witness: role of recombinant human factor VIIa.

    Science.gov (United States)

    Mindikoglu, Ayse L; Anantharaju, Abhinandana; George, Magdalene; Leone, Nancy; Bejna, Judy; Van Thiel, David H

    2003-01-01

    A case of a 50-year-old Jehovah's Witness with cryptogenic cirrhosis, severe portal hypertension and a coagulopathy, who underwent splenic embolization to improve the platelet count after receiving recombinant human Factor VIIa, is reported. Following the infusion of recombinant human Factor VIIa, the coagulopathy was rapidly corrected and it became possible to safely embolize her spleen. The changes in prothrombin time, international normalized ratio and activated partial thromboplastin time as well as thrombomodulin, tissue factor and plasminogen activator inhibitor after the infusion are presented. As a result of the splenic embolization, her platelet count normalized and she has been listed for liver transplantation.

  16. Efficacy of recombinant factor VIIa administered by continuous infusion to haemophilia patients with inhibitors

    NARCIS (Netherlands)

    Mauser-Bunschoten, EP; Koopman, MMW; Goede-Bolder, ADE; Leebeek, FWG; Van der Meer, J; Kooij, GMV; Van der Linden, PWG

    We have prospectively monitored treatment of haemophilia patients with inhibitors by recombinant factor VIIa (rFVIIa) administered by continuous infusion to obtain more insight in the underlying factors of the clinical efficacy of this administration method. At present, 43 treatment episodes of 14

  17. Mechanism of the Ca2+-induced enhancement of the intrinsic factor VIIa activity

    DEFF Research Database (Denmark)

    Bjelke, Jais R; Olsen, Ole H; Fodje, Michel

    2008-01-01

    The intrinsic activity of coagulation factor VIIa (FVIIa) is dependent on Ca(2+) binding to a loop (residues 210-220) in the protease domain. Structural analysis revealed that Ca(2+) may enhance the activity by attenuating electrostatic repulsion of Glu(296) and/or by facilitating interactions...... by a combination of charge neutralization and loop stabilization....

  18. Influence of cardiopulmonary bypass on the interaction of recombinant factor VIIa with activated platelets

    DEFF Research Database (Denmark)

    Kjalke, M.; Runge, M.; Rojkjaer, R.

    2009-01-01

    Recombinant factor VIIa (rFVIIa) interacts preferentially with coated platelets characterized by a high exposure of phosphatidyl serine (PS), FV, FVIII, FIX, and FX binding, and fibrinogen. Cardiopulmonary bypass (CPB) is known to impair platelet function. In this study, the influence of CPB...

  19. In Silico Design of Novel Anticoagulant Peptides targeting Blood Coagulation Factor VIIa.

    Science.gov (United States)

    Al-Amri, Manal S Q; Alrasadi, Khalid; Bayoumi, Riad; Banerjee, Yajnavalka

    2011-02-01

    The coagulation cascade initiated during vascular injury prevents bleeding. Unwanted clot formation is however detrimental and requires the use of anticoagulants for prophylaxis and treatment. Anticoagulants targeting a specific step or an enzyme in the clotting process are most preferred as they minimise disadvantageous side-effects. A principal step in the discovery of novel anticoagulants encompasses the in silico design of potential leads. This study depicts the in silico design of peptide anticoagulants targeting coagulation factor VIIa. APPLYING THE PROLINE BRACKET RULE AND USING VARIOUS BIOINFORMATICS TOOLS: the basic alignment search tool (BLAST) of National Center for Biotechnology Information; the T-coffee module provided by European Molecular Biology Laboratory-European Bioinformatics Institute, and several modules available on the ExPASy server, we designed five bivalent chimeric anticoagulants targeting factor VIIa, using factor VIIa inhibitors - hemextin A from Hemachatus haemachatus (African Ringhals cobra) venom and factor VIIa exosite-inhibitor peptide as templates. Six peptides were derived from hemextin A, which were concomitantly fused with factor VIIa exosite-inhibitor peptide intermediated by a polyalanine spacer, and analysed for structural stability using the SWISS-MODEL software developed at the Swiss Institute of Bioinformatics and WebLab ViewerPro (Version 4.2). Twelve chimeric peptides were obtained; only five exhibited stable structures in silico. The five peptides obtained are probable anticoagulant leads that should be further evaluated using suitable in vitro and in vivo assays. Further, this study shows how simple web-based modules can be used for the rational design of probable leads targeting specific physiological molecular targets.

  20. Louisiana Marinas and Boat Launches, Geographic NAD83, LOSCO (2004) [marinas_LOSCO_2004

    Data.gov (United States)

    Louisiana Geographic Information Center — The dataset defines the location and supplemental information for marinas and boat launches in southern Louisiana. The boat launch database includes public and...

  1. Extensive small-angle X-ray scattering studies of blood coagulation factor VIIa reveal interdomain flexibility

    DEFF Research Database (Denmark)

    Mosbæk, Charlotte Rode; Nolan, David; Persson, Egon

    2010-01-01

    Blood coagulation factor VIIa (FVIIa) is used in the treatment of replacement therapy resistant hemophilia patients, and FVIIa is normally activated upon complex formation with tissue factor (TF), potentially in context with structural rearrangements. The solution behavior of uncomplexed FVIIa...

  2. CORPORATE SOCIAL RESPONSIBILITY OF CROATIAN MARINAS

    National Research Council Canada - National Science Library

    Sara Klaric; Kristina Afric Rakitovac; Klara Trost Lesic

    2015-01-01

    .... In the modern business world, the significance of corporate social responsibility (CSR) is increasing. All marina businesses should, therefore, be measured based on their contribution to the economic progress, ecological quality and social capital...

  3. Crystal Structures of Human Group-VIIA Phospholipase A2 Inhibited by Organophosphorus Nerve Agents Exhibit Non-aged Complexes

    OpenAIRE

    Samanta, Uttamkumar; Kirby, Stephen D.; Srinivasan, Prabhavathi; Douglas M Cerasoli; Bahnson, Brian J.

    2009-01-01

    Abstract The enzyme group-VIIA phospholipase A2 (gVIIA-PLA2) is bound to lipoproteins in human blood and hydrolyzes the ester bond at the sn-2 position of phospholipid substrates with a short sn-2 chain. The enzyme belongs to a serine hydrolase superfamily of enzymes, which react with organophosphorus (OP) nerve agents. OPs ultimately exert their toxicity by inhibiting human acetycholinesterase at nerve synapses, but may additionally have detrimental effects through inhibition of o...

  4. MyosinVIIa interacts with Twinfilin-2 at the tips of mechanosensory stereocilia in the inner ear.

    Directory of Open Access Journals (Sweden)

    Agnieszka K Rzadzinska

    Full Text Available In vertebrates hearing is dependent upon the microvilli-like mechanosensory stereocilia and their length gradation. The staircase-like organization of the stereocilia bundle is dynamically maintained by variable actin turnover rates. Two unconventional myosins were previously implicated in stereocilia length regulation but the mechanisms of their action remain unknown. MyosinXVa is expressed in stereocilia tips at levels proportional to stereocilia length and its absence produces staircase-like bundles of very short stereocilia. MyosinVIIa localizes to the tips of the shorter stereocilia within bundles, and when absent, the stereocilia are abnormally long. We show here that myosinVIIa interacts with twinfilin-2, an actin binding protein, which inhibits actin polymerization at the barbed end of the filament, and that twinfilin localization in stereocilia overlaps with myosinVIIa. Exogenous expression of myosinVIIa in fibroblasts results in a reduced number of filopodia and promotes accumulation of twinfilin-2 at the filopodia tips. We hypothesize that the newly described interaction between myosinVIIa and twinfilin-2 is responsible for the establishment and maintenance of slower rates of actin turnover in shorter stereocilia, and that interplay between complexes of myosinVIIa/twinfilin-2 and myosinXVa/whirlin is responsible for stereocilia length gradation within the bundle staircase.

  5. Analüütikud soovitavad Eesti Energia börsile viia / Piret Reiljan

    Index Scriptorium Estoniae

    Reiljan, Piret, 1983-

    2008-01-01

    Kohalikud analüütikud soovitavad viia Eesti Energia börsile, kuna see elavdaks aktsiaturgu ja tõmbaks ligi välisinvestoreid. Vt. samas: Raivo Vare: raske aeg töötab Eesti Energia börsiletoomise kasuks; Analüüs ootab pääsu valitsuskabinetti; Juhid ei püsi enam Eesti Energias. Diagrammid: Majandusnäitajad; Varade maht

  6. Patrimonio Natural y Reservas Marinas

    Directory of Open Access Journals (Sweden)

    Raquel de la Cruz Modino

    2004-01-01

    Full Text Available Las reservas marinas intentan preservar ciertas zonas de especial interés biológico de los excesos de la pesca con el fin de asegurar la protección, la regeneración y el desarrollo de los recursos marinos. Con ellas se aspira a regular las diferentes actividades que en estas áreas se llevan a cabo y conjugar los usos turísticos y recreativos que se pueden realizar con la conservación de sus valores naturales. Sin embargo, por norma general, la regulación sobre los usos que pueden desarrollarse en áreas naturales especialmente frágiles como éstas, se restringe a establecer una serie de limitaciones sobre las actividades que son llevadas a cabo en la zona. La implantación de figuras como éstas, dentro de un contexto turístico, puede alentar y promover el desarrollo de actividades recreativas frente a los usos tradicionales, que han podido quedar limitados en el área protegida

  7. Tissue factor activates allosteric networks in factor VIIa through structural and dynamic changes

    DEFF Research Database (Denmark)

    Madsen, Jesper Jonasson; Persson, E.; Olsen, O. H.

    2015-01-01

    Background: Tissue factor (TF) promotes colocalization of enzyme (factorVIIa) and substrate (FX or FIX), and stabilizes the active conformation of FVIIa. Details on how TF induces structural and dynamic changes in the catalytic domain of FVIIa to enhance its efficiency remain elusive. Objective......: To elucidate the activation of allosteric networks in the catalytic domain of the FVIIa protease it is when bound to TF.MethodsLong-timescale molecular dynamics simulations of FVIIa, free and in complex with TF, were executed and analyzed by dynamic network analysis. Results: Allosteric paths of correlated...

  8. Synthesis and P1' SAR exploration of potent macrocyclic tissue factor-factor VIIa inhibitors

    Energy Technology Data Exchange (ETDEWEB)

    Ladziata, Vladimir (Uladzimir); Glunz, Peter W.; Zou†, Yan; Zhang, Xiaojun; Jiang, Wen; Jacutin-Porte, Swanee; Cheney, Daniel L.; Wei, Anzhi; Luettgen, Joseph M.; Harper, Timothy M.; Wong, Pancras C.; Seiffert, Dietmar; Wexler, Ruth R.; Priestley, E. Scott (BMS)

    2016-10-01

    Selective tissue factor-factor VIIa complex (TF-FVIIa) inhibitors are viewed as promising compounds for treating thrombotic disease. In this contribution, we describe multifaceted exploratory SAR studies of S1'-binding moieties within a macrocyclic chemotype aimed at replacing cyclopropyl sulfone P1' group. Over the course of the optimization efforts, the 1-(1H-tetrazol-5-yl)cyclopropane P1' substituent emerged as an improved alternative, offering increased metabolic stability and lower clearance, while maintaining excellent potency and selectivity.

  9. Perspectivas de la biología marina

    OpenAIRE

    Bigatti, G.

    2013-01-01

    La biología marina es una rama de las ciencias del mar de reciente desarrollo en la Argentina (oceáno Atlántico sudoccidental) que está en franco crecimiento. Se muestra un panorama del desarrollo de las diversas disciplinas, como los estudios sobre la biodiversidad marina y de la biología marina y pesquera, así como amenazas a los ambientes marinos y medidas de conservación tomadas. Se incluyen las perspectivas para futuros estudios de biología marina, los beneficios de las iniciativas de di...

  10. The Shaker-1 Mouse Myosin VIIa Deafness Mutation Results in a Severely Reduced Rate of the ATP Hydrolysis Step.

    Science.gov (United States)

    Xiong, Ailian; Haithcock, Jessica; Liu, Yingying; Eusner, Lauren; McConnell, Matthew; White, Howard D; Belknap, Betty; Forgacs, Eva

    2017-11-22

    Mutations in the MYO7A gene, encoding the motor protein myosin VIIa, can cause Usher 1B, a deafness/blindness syndrome in humans, and the shaker-1 phenotype, characterized by deafness, head tossing and circling behavior, in mice. Myosin VIIa is responsible for tension bearing and the transduction mechanism in the stereocilia and for melanosome transport in the retina, in line with the phenotypic outcomes observed in mice. However, the effect of the shaker-1 mutation, a R502P amino acid substitution, on the motor function is unclear. To explore this question, we determined the kinetic properties and the effect on the filopodial tip localization of the recombinant mouse myosin VIIa-5IQ-SAH R502P (MyoVIIa-sh1) construct. Interestingly, though residue 502 is localized to a region thought to be involved in interacting with actin, the kinetic parameters for actin binding changed only slightly for the mutant construct. However, the rate constant for ATP hydrolysis (k+H + k-H) was reduced by ~ 200 fold from 12 s-1 to 0.05 s-1 making the hydrolysis step the rate limiting step of the ATPase cycle in the presence and absence of actin. Given that wild type mouse myosin VIIa is a slow, high duty ratio, monomeric motor, this altered hydrolysis rate would reduce activity to extremely low levels. Indeed, the translocation to the filopodial tips was hampered by the diminished motor function of a dimeric construct of the shaker-1 mutant. We conclude that the diminished motor activity of this mutant is most likely responsible for impaired hearing in the shaker-1 mice. Copyright © 2017, The American Society for Biochemistry and Molecular Biology.

  11. Human recombinant factor VIIa may improve heat intolerance in mice by attenuating hypothalamic neuronal apoptosis and damage.

    Science.gov (United States)

    Hsu, Chuan-Chih; Chen, Sheng-Hsien; Lin, Cheng-Hsien; Yung, Ming-Chi

    2014-10-01

    Intolerance to heat exposure is believed to be associated with hypothalamo-pituitary-adrenocortical (HPA) axis impairment [reflected by decreases in blood concentrations of both adrenocorticotrophic-hormone (ACTH) and corticosterone]. The purpose of this study was to determine the effect of human recombinant factor VIIa (rfVIIa) on heat intolerance, HPA axis impairment, and hypothalamic inflammation, ischemic and oxidative damage, and apoptosis in mice under heat stress. Immediately after heat stress (41.2 °C for 1 h), mice were treated with vehicle (1 mL/kg of body weight) or rfVIIa (65-270 µg/kg of body weight) and then returned to room temperature (26 °C). Mice still alive on day 4 of heat exposure were considered survivors. Cellular ischemia markers (e.g., glutamate, lactate-to-pyruvate ratio), oxidative damage markers (e.g., nitric oxide metabolite, hydroxyl radials), and pro-inflammatory cytokines (e.g., interleukin-6, interleukin-1β, tumor necrosis factor-α) in hypothalamus were determined. In addition, blood concentrations of both ACTH and corticosterone were measured. Hypothalamic cell damage was assessed by determing the neuronal damage scores, whereas the hypothalamic cell apoptosis was determined by assessing the numbers of cells stained with terminal deoxynucleotidyl transferase-mediated αUTP nick-end labeling, caspase-3-positive cells, and platelet endothelial cell adhesion molecula-1-positive cells in hypothalamus. Compared with vehicle-treated heated mice, rfVIIa-treated heated mice had significantly higher fractional survival (8/10 vs 1/10), lesser thermoregulatory deficit (34.1 vs 24.8 °C), lesser extents of ischemic, oxidative, and inflammatory markers in hypothalamus, lesser neuronal damage scores and apoptosis in hypothalamus, and lesser HPA axis impairment. Human recombinant factor VIIa appears to exert a protective effect against heatstroke by attenuating hypothalamic cell apoptosis (due to ischemic, inflammatory, and oxidative damage

  12. Marina Laikjõe : palm on eestlasele puhkuse sünonüüm / Marina Laikjõe

    Index Scriptorium Estoniae

    Laikjõe, Marina, 1967-

    2003-01-01

    Reisikorraldusfirma Domina World Traveli tegevjuht Marina Laikjõe annab ülevaate eestlaste reisimisharjumustest, populaarsematest sihtkohtadest ning prognoosib võimalikke trende Eesti turismiturul.

  13. Recombinant Factor VIIa (Eptacog Alfa): A Pharmacoeconomic Review of its Use in Haemophilia in Patients with Inhibitors to Clotting Factors VIII or IX

    OpenAIRE

    Katherine A. Lyseng-Williamson; Greg L. Plosker

    2007-01-01

    Recombinant factor VIIa (NovoSeven(R); also known as recombinant activated factor VII or eptacog alfa) is indicated as an intravenous haemostatic agent in haemophilia patients with inhibitors to clotting factors VIII or IX. In noncomparative trials in haemophilia patients with inhibitors, on-demand home treatment with recombinant factor VIIa was effective in controlling episodes of mild to moderate bleeding and well tolerated, with early treatment being associated with a greater rate of succe...

  14. Marina Kaljurand : Venemaad ei tohi karta! Siin pole midagi karta! / Marina Kaljurand ; interv. Jaanus Piirsalu

    Index Scriptorium Estoniae

    Kaljurand, Marina, 1962-

    2008-01-01

    Intervjuu kolm aastat Moskvas töötanud Eesti suursaadiku Marina Kaljurannaga, kes vastab küsimustele, mis puudutavad Eesti-Vene suhteid, saadikutööd, diplomaatiat, aprillirahutusi, naiste osakaalu diplomaatide seas ja erilisi suhteid patriarh Aleksius II-ga

  15. Armastus teeb tugevaks / Marina Laikjõe ; interv. Verni Leivak

    Index Scriptorium Estoniae

    Laikjõe, Marina, 1967-

    2008-01-01

    Turismifirma Tez Tour tegevjuht ning reisifirma Travel-In juht Marina Laikjõe vastab küsimustele, mis puudutavad tema tegevust ja karjääri turisminduses, suhteid kolleegidega ning perekonna toetust raske haigusega võitlemise ajal. Kommenteerivad Reet Kivi, Tiit Pärnik. Lisa: Marina Laikjõe

  16. Contaminación marina en el Perú.

    OpenAIRE

    Guillen., Oscar; Ashtu, Victor; Aquino, Rosa

    1980-01-01

    Este informe muestra las principales causas de contaminación marina debido a los desechos domésticos e industriales, hidrocarburos de petroleo a fin de mostrar la situación actual de la contaminación marina.

  17. Postpartum hemorrhage in a Jehovah's Witness patient controlled with Tisseel, tranexamic acid, and recombinant factor VIIa.

    Science.gov (United States)

    Arab, Tarek Samir; Al-Wazzan, Ahmad Bakr; Maslow, Ken

    2010-10-01

    The management of a patient refusing blood transfusion who subsequently experiences a severe postpartum hemorrhage is a particular clinical challenge. A 30-year-old nulliparous patient (who was a Jehovah's Witness) had labour induced for post-dates at 41+4 weeks' gestational age after an uncomplicated pregnancy. She delivered by Caesarean section for dystocia and suspected chorioamnionitis, and subsequently developed postpartum hemorrhage that required management with oxytocin, ergometrine, carboprost, uterine artery ligation, and Hayman compression sutures. The patient ultimately required two additional visits to the operating room, culminating in hysterectomy. Use of tranexamic acid, recombinant factor VIIa, and Tisseel was instrumental in halting the ongoing hemorrhage. Optimal management of a patient refusing administration of blood products requires a multidisciplinary approach as well as a combination of traditional and novel therapies.

  18. Factor VIIa and tissue factor--from cell biology to animal models.

    Science.gov (United States)

    Rao, L Vijaya Mohan; Mackman, Nigel

    2010-04-01

    Binding of factor VIIa (FVIIa) to tissue factor (TF) and the subsequent initiation of the clotting cascade is essential for hemostasis. However, the aberrant expression of FVIIa-TF contributes to thrombosis. Despite the tremendous progress made in the past 25years in understanding the molecular mechanisms involved in the interaction between FVIIa and TF, there is less known about the cell biology of these proteins. Availability of hemophilic mice (by specific knock-out of FVIII or FIX genes) and novel TF transgenic mice has allowed us in recent years to investigate the importance of TF-FVIIa-induced coagulation from wound healing to sepsis. This supplement explores new aspects of TF-FVIIa biology, with a particular focus on structural biology, cell biology and animal models. Copyright (c) 2010 Elsevier Ltd. All rights reserved.

  19. Myosin VIIA mutation screening in 189 Usher syndrome type 1 patients

    Energy Technology Data Exchange (ETDEWEB)

    Weston, M.D.; Kelley, P.M.; Overbeck, L.D. [Univ. of Nebraska Medical Center, Omaha, NE (United States)] [and others

    1996-11-01

    Usher syndrome type 1b (USH1B) is an autosomal recessive disorder characterized by congenital profound hearing loss, vestibular abnormalities, and retinitis pigmentosa. The disorder has recently been shown to be caused by mutations in the myosin VIIa gene (MYO7A) located on 11q14. In the current study, a panel of 189 genetically independent Usher I cases were screened for the presence of mutations in the N-terminal coding portion of the motor domain of MYO7A by heteroduplex analysis of 14 exons. Twenty-three mutations were found segregating with the disease in 20 families. Of the 23 mutations, 13 were unique, and 2 of the 13 unique mutations (Arg212His and Arg212Cys) accounted for the greatest percentage of observed mutant alleles (8/23, 31%). Six of the 13 mutations caused premature stop codons, 6 caused changes in the amino acid sequence of the myosin VIIa protein, and 1 resulted in a splicing defect. Three patients were homozygotes or compound heterozygotes for mutant alleles; these three cases were Tyr333Stop/Tyr333Stop, Arg212His-Arg302His/Arg212His-Arg302His, and IVS13nt-8c{r_arrow}g/ G1u450Gln. All the other USH1B mutations observed were simple heterozygotes, and it is presumed that the mutation on the other allele is present in the unscreened regions of the gene. None of the mutations reported here were observed in 96 unrelated control samples, although several polymorphisms were detected. These results add three patients to a single case reported previously where mutations have been found in both alleles and raises the total number of unique mutations in MYO7A to 16. 22 refs., 4 figs., 3 tabs.

  20. Nuevos registros de clorofitas marinas para Cuba

    OpenAIRE

    Moreira, A.; Fujii, M. T.; Suárez, A. M.; Wynne, M. J.

    2013-01-01

    A partir de muestras colectadas en la bahía de Cienfuegos y de Santa Lucía (Pinar del Río), se identificaron dos nuevos registros de macroalgas marinas (clorofitas) para Cuba: Cladophora blomquistii C. Hoek y Udotea fibrosa D. S. Littler & M. M. Littler. C. blomquistii se registra por primera vez para la región del Caribe ya que se había consignado solo para lagunas costeras de la Florida, y la parte noreste del golfo de México. Se ofrecen además comentarios sobre la taxonomía, ...

  1. The Clinical and Laboratory Response to Recombinant Factor VIIa in Trauma and Surgical Patients with Acquired Coagulopathy

    Science.gov (United States)

    2006-08-01

    difficult, if not impossible, to interrupt. Standard transfusion therapy is often inadequate to deal with the challenges of the progression of acquired...reported to the FDA’s Adverse Events Reporting System ( AERS ). The focus of this article is the off-label use of rFVIIa and the number of serious...hemorrhage model. Shock. 2004;22:163-168. 11. Boffard KD, Riou B, Warren B, et al. Recombinant factor VIIa as adjunctive therapy for bleeding control

  2. Crystal structures of human group-VIIA phospholipase A2 inhibited by organophosphorus nerve agents exhibit non-aged complexes

    Energy Technology Data Exchange (ETDEWEB)

    Samanta, Uttamkumar; Kirby, Stephen D.; Srinivasan, Prabhavathi; Cerasoli, Douglas M.; Bahnson, Brian J.; (Delaware); (USAMRIID)

    2009-09-02

    The enzyme group-VIIA phospholipase A2 (gVIIA-PLA2) is bound to lipoproteins in human blood and hydrolyzes the ester bond at the sn-2 position of phospholipid substrates with a short sn-2 chain. The enzyme belongs to a serine hydrolase superfamily of enzymes, which react with organophosphorus (OP) nerve agents. OPs ultimately exert their toxicity by inhibiting human acetycholinesterase at nerve synapses, but may additionally have detrimental effects through inhibition of other serine hydrolases. We have solved the crystal structures of gVIIA-PLA2 following inhibition with the OPs diisopropylfluorophosphate, sarin, soman and tabun. The sarin and soman complexes displayed a racemic mix of P{sub R} and P{sub S} stereoisomers at the P-chiral center. The tabun complex displayed only the P{sub R} stereoisomer in the crystal. In all cases, the crystal structures contained intact OP adducts that had not aged. Aging refers to a secondary process OP complexes can go through, which dealkylates the nerve agent adduct and results in a form that is highly resistant to either spontaneous or oxime-mediated reactivation. Non-aged OP complexes of the enzyme were corroborated by trypsin digest and matrix-assisted laser desorption ionization mass spectrometry of OP-enzyme complexes. The lack of stereoselectivity of sarin reaction was confirmed by gas chromatography/mass spectrometry using a chiral column to separate and quantitate the unbound stereoisomers of sarin following incubation with enzyme. The structural details and characterization of nascent reactivity of several toxic nerve agents is discussed with a long-term goal of developing gVIIA-PLA2 as a catalytic bioscavenger of OP nerve agents.

  3. A New Heterotrophic Cryptomonad: Hemiarma marina n. g., n. sp.

    Science.gov (United States)

    Shiratori, Takashi; Ishida, Ken-Ichiro

    2016-11-01

    We report a new heterotrophic cryptomonad Hemiarma marina n. g., n. sp. that was collected from a seaweed sample from the Republic of Palau. In our molecular phylogenetic analyses using the small subunit ribosomal RNA gene, H. marina formed a clade with two marine environmental sequences, and the clade was placed as a sister lineage of the freshwater cryptomonad environmental clade CRY1. Alternatively, in the concatenated large and small subunit ribosomal RNA gene phylogeny, H. marina was placed as a sister lineage of Goniomonas. Light and electron microscopic observations showed that H. marina shares several ultrastructural features with cryptomonads, such as flattened mitochondrial cristae, a periplast cell covering, and ejectisomes that consist of two coiled ribbon structures. On the other hand, H. marina exhibited unique behaviors, such as attaching to substrates with its posterior flagellum and displaying a jumping motion. H. marina also had unique periplast arrangement and flagellar transitional region. On the basis of both molecular and morphological information, we concluded that H. marina should be treated as new genus and species of cryptomonads. © 2016 The Author(s) Journal of Eukaryotic Microbiology © 2016 International Society of Protistologists.

  4. Hematologic effects of recombinant factor VIIa combined with hemoglobin-based oxygen carrier-201 for prehospital resuscitation of swine with severe uncontrolled hemorrhage due to liver injury.

    Science.gov (United States)

    Arnaud, Françoise; Hammett, Michael; Philbin, Nora; Scultetus, Anke; McCarron, Richard; Freilich, Daniel

    2008-10-01

    The combination of traumatic injury, hemorrhage, and fluid resuscitation results in consumption and dilution of coagulation factors, adversely impacting hematology outcome in trauma patients. The hemostatic effects of escalating doses of recombinant factor VIIa added to hemoglobin-based oxygen carrier-201 were assessed as prehospital fluid resuscitation in swine with severe uncontrolled hemorrhage. Swine underwent liver injury causing severe uncontrolled hemorrhage and shock. During a 4-h prehospital phase, either hypotensive or tachycardic, or both, animals were resuscitated with hemoglobin-based oxygen carrier-201 without (0x) or with escalating doses of recombinant factor VIIa [90 microg/kg (1x), 180 microg/kg (2x), or 360 microg/kg (4x)]. The animals received one initial full dose of 10 ml/kg at 15 min and up to four doses of 5 ml/kg thereafter. From 4 to 72 h (hospital phase), animals received either transfusions or isotonic saline or both as needed. Hematology profile (complete blood count), thromboelastography, in-vitro bleeding (platelet function analyzer), and coagulation (prothrombin time) were measured and the results were compared using mixed statistical models. In all groups, dilutional coagulopathy was evidenced by reduced hematocrit, platelets, and thromboelastography-maximum amplitude, and increased platelet function analyzer closure time and thromboelastography-reaction time. During the prehospital phase, hemoglobin-based oxygen carrier-201 restored hemoglobin in all groups. Recombinant factor VIIa decreased prothrombin time in recombinant factor VIIa groups compared with the hemoglobin-based oxygen carrier-201 group (P 0.05). Compared with hemoglobin-based oxygen carrier, 1x recombinant factor VIIa tended to decrease blood loss, lactate and thromboelastography-reaction time at 24 h but the 4x group increased these parameters. Platelets and thromboelastography-maximum amplitude decreased (P hemoglobin-based oxygen carrier-201 did not change

  5. The M358R variant of α{sub 1}-proteinase inhibitor inhibits coagulation factor VIIa

    Energy Technology Data Exchange (ETDEWEB)

    Sheffield, William P., E-mail: sheffiel@mcmaster.ca [Canadian Blood Services, Centre for Innovation, Hamilton, Ontario (Canada); Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario (Canada); Bhakta, Varsha [Canadian Blood Services, Centre for Innovation, Hamilton, Ontario (Canada)

    2016-02-12

    The naturally occurring M358R mutation of the plasma serpin α{sub 1}-proteinase inhibitor (API) changes both its cleavable reactive centre bond to Arg–Ser and the efficacy with which it inhibits different proteases, reducing the rate of inhibition of neutrophil elastase, and enhancing that of thrombin, factor XIa, and kallikrein, by several orders of magnitude. Although another plasma serpin with an Arg–Ser reactive centre, antithrombin (AT), has been shown to inhibit factor VIIa (FVIIa), no published data are available with respect to FVIIa inhibition by API M358R. Recombinant bacterially-expressed API M358R and plasma-derived AT were therefore compared using gel-based and kinetic assays of FVIIa integrity and activity. Under pseudo-first order conditions of excess serpin over protease, both AT and API M358R formed denaturation-resistant inhibitory complexes with FVIIa in reactions accelerated by TF; AT, but not API M358R, also required heparin for maximal activity. The second order rate constant for heparin-independent API M358R-mediated FVIIa inhibition was determined to be 7.8 ± 0.8 × 10{sup 2} M{sup −1}sec{sup −1}. We conclude that API M358R inhibits FVIIa by forming inhibitory complexes of the serpin type more rapidly than AT in the absence of heparin. The likely 20-fold excess of API M358R over AT in patient plasma during inflammation raises the possibility that it could contribute to the hemorrhagic tendencies manifested by rare individuals expressing this mutant serpin. - Highlights: • The inhibitory specificity of the serpin alpha-1-proteinase inhibitor (API) is sharply altered in the M358R variant. • API M358R forms denaturation-resistant complexes with coagulation factor VIIa at a rate accelerated by tissue factor but unaffected by heparin. • Complex formation was shown by gel-based assays and quantified kinetically by inhibition of FVIIa-dependent amidolysis.

  6. Efficacy of Avicennia marina (Forsk.) Vierh. leaves extracts against ...

    African Journals Online (AJOL)

    Efficacy of Avicennia marina (Forsk.) Vierh. leaves extracts against some atmospheric fungi. M Afzal, FS Mehdi, FM Abbasi, H Ahmad, R Masood, J Alam, G Jan, M Islam, NU Amin, A Majid, M Fiaz, AH Shah ...

  7. Eelgrass (Zostera marina) Food Web Structure in Different Environmental Settings

    National Research Council Canada - National Science Library

    Thormar, Jonas; Hasler-Sheetal, Harald; Baden, Susanne; Boström, Christoffer; Clausen, Kevin Kuhlmann; Krause-Jensen, Dorte; Olesen, Birgit; Rasmussen, Jonas Ribergaard; Svensson, Carl Johan; Holmer, Marianne

    2016-01-01

    This study compares the structure of eelgrass (Zostera marina L.) meadows and associated food webs in two eelgrass habitats in Denmark, differing in exposure, connection to the open sea, nutrient enrichment and water transparency...

  8. A new record of Spergularia marina (Caryophyllaceae from southern Poland

    Directory of Open Access Journals (Sweden)

    Pliszko Artur

    2017-03-01

    Full Text Available Spergularia marina, a rare native halophyte and nationally vulnerable species, is reported from a new locality in southern Poland. It was found on 14 September 2016 in Mogilany, Lesser Poland Voivodeship, growing between paving stones at the Zakopianka road. Map of distribution of S. marina in Poland based on the ATPOL cartogram method is provided, and its possible pathways of introduction are discussed.

  9. Esteroles libres de la estrella marina echinaster sentus

    Directory of Open Access Journals (Sweden)

    Rosabel Segura de Correa

    2010-05-01

    Full Text Available La fracción lipídica de la estrella marina Echinaster sentus se estudió para conocer su composición esterólica. Las estrellas marinas son especies de invertebrados-que pertenecen al phylumechinodermata de la clase Asteroidea. Poseen entre otros componentes esteroides de tipo saponina, esteróles libres polihidroxilados y monohidroxilados.

  10. Myosin VIIA regulates microvillus morphogenesis and interacts with cadherin Cad99C in Drosophila oogenesis.

    Science.gov (United States)

    Glowinski, Cory; Liu, Ri-Hua Sandy; Chen, Xi; Darabie, Audrey; Godt, Dorothea

    2014-11-15

    Microvilli and related actin-based protrusions permit multiple interactions between cells and their environment. How the shape, length and arrangement of microvilli are determined remains largely unclear. To address this issue and explore the cooperation of the two main components of a microvillus, the central F-actin bundle and the enveloping plasma membrane, we investigated the expression and function of Myosin VIIA (Myo7A), which is encoded by crinkled (ck), and its interaction with cadherin Cad99C in the microvilli of the Drosophila follicular epithelium. Myo7A is present in the microvilli and terminal web of follicle cells, and associates with several other F-actin-rich structures in the ovary. Loss of Myo7A caused brush border defects and a reduction in the amount of the microvillus regulator Cad99C. We show that Myo7A and Cad99C form a molecular complex and that the cytoplasmic tail of Cad99C recruits Myo7A to microvilli. Our data indicate that Myo7A regulates the structure and spacing of microvilli, and interacts with Cad99C in vivo. A comparison of the mutant phenotypes suggests that Myo7A and Cad99C have co-dependent and independent functions in microvilli. © 2014. Published by The Company of Biologists Ltd.

  11. Successful Treatment of Intractable Hemothorax with Recombinant Factor VIIa in a Nonhemophilic Patient

    Directory of Open Access Journals (Sweden)

    Yu-Feng Wei

    2006-01-01

    Full Text Available Recombinant factor VIIa (rFVIIa was developed for the treatment of bleeding in hemophilic patients with inhibitors. It has also been used to stop bleeding in nonhemophilic patients who fail to respond to conventional treatment. We report a case of catastrophic hemothorax in which bleeding was stopped by administration of rFVIIa. A 68-year-old woman with chronic hepatitis C-related liver cirrhosis was admitted due to pneumonia and parapneumonic effusion. The patient developed hemothorax and hypovolemic shock after thoracentesis. Conventional therapies including tube thoracostomy and transarterial embolization failed to stop the life-threatening bleeding. The bleeding stopped after administration of rFVIIa 100 μg/kg/BW at 2-hour intervals for a total of two doses on the 3rd day of hospitalization. Despite intensive care, however, the patient died due to nosocomial infection and multiple organ failure on the 12th day of hospitalization. Hemothorax in a nonhemophilic patient can be successfully treated with rFVIIa.

  12. Recombinant factor VIIa (eptacog alfa): a pharmacoeconomic review of its use in haemophilia in patients with inhibitors to clotting factors VIII or IX.

    Science.gov (United States)

    Lyseng-Williamson, Katherine A; Plosker, Greg L

    2007-01-01

    Recombinant factor VIIa (NovoSeven; also known as recombinant activated factor VII or eptacog alfa) is indicated as an intravenous haemostatic agent in haemophilia patients with inhibitors to clotting factors VIII or IX. In noncomparative trials in haemophilia patients with inhibitors, on-demand home treatment with recombinant factor VIIa was effective in controlling episodes of mild to moderate bleeding and well tolerated, with early treatment being associated with a greater rate of success and the need for fewer doses than delayed treatment. Prophylactic treatment with recombinant factor VIIa was also effective in maintaining haemostasis in patients with this indication undergoing surgery. Relative to prior treatment with plasma-derived agents, treatment with recombinant factor VIIa was associated with improvements in health-related quality of life in a cost-utility study in haemophilia patients with inhibitors in Australia. In well designed decision-model cost analyses conducted from a healthcare payer perspective in several countries, on-demand treatment with recombinant factor VIIa to control mild to moderate bleeding episodes in this patient population was predicted to be cost saving or cost neutral relative to on-demand treatment with intravenous activated prothrombin complex concentrate (aPCC). Although the acquisition cost of recombinant factor VIIa was greater than that of aPCC in some studies, the greater initial efficacy of recombinant factor VIIa than aPCC resulted in lower predicted total medical costs. Results were generally robust to plausible changes in key parameters. Orthopaedic surgery with recombinant factor VIIa to maintain haemostasis in haemophilia patients with inhibitors was generally predicted to be cost saving, relative to not having surgery, over the medium to long term in modelled cost analyses from a healthcare payer perspective in the UK and US. The initial cost of surgery was high, but the difference in costs between patients

  13. The origins of enhanced activity in factor VIIa analogs and the interplay between key allosteric sites revealed by hydrogen exchange mass spectrometry

    DEFF Research Database (Denmark)

    Rand, Kasper D; Andersen, Mette D; Olsen, Ole H

    2008-01-01

    Factor VIIa (FVIIa) circulates in the blood in a zymogen-like state. Only upon association with membrane-bound tissue factor (TF) at the site of vascular injury does FVIIa become active and able to initiate blood coagulation. Here we used hydrogen exchange monitored by mass spectrometry to invest......Factor VIIa (FVIIa) circulates in the blood in a zymogen-like state. Only upon association with membrane-bound tissue factor (TF) at the site of vascular injury does FVIIa become active and able to initiate blood coagulation. Here we used hydrogen exchange monitored by mass spectrometry...

  14. A combined structural dynamics approach identifies a putative switch in factor VIIa employed by tissue factor to initiate blood coagulation

    DEFF Research Database (Denmark)

    Olsen, Ole H; Rand, Kasper D; Østergaard, Henrik

    2007-01-01

    Coagulation factor VIIa (FVIIa) requires tissue factor (TF) to attain full catalytic competency and to initiate blood coagulation. In this study, the mechanism by which TF allosterically activates FVIIa is investigated by a structural dynamics approach that combines molecular dynamics (MD......) simulations and hydrogen/deuterium exchange (HX) mass spectrometry on free and TF-bound FVIIa. The differences in conformational dynamics from MD simulations are shown to be confined to regions of FVIIa observed to undergo structural stabilization as judged by HX experiments, especially implicating activation...

  15. Bioaccumulation of trace elements by Avicennia marina

    Directory of Open Access Journals (Sweden)

    Kandasamy Kathiresan

    2014-11-01

    Full Text Available Objective: To analyze the concentrations of 12 micro-nutrients (Al, B, Cd, Co, Cr, Cu, Fe, Mg, Mn, Ni, Pb, and Zn in different plant parts of Avicennia marina and its rhizosphere soil of the south east coast of India. Methods: The samples were acid digested, then analyzed by using inductively coupled plasma system (ICP-Optical Emission Spectrophotometer. Results: Levels of metals were found in the decreasing order: Cd>Co>Ni>Pb>B >Cr>Zn>Mg>Mn>Cu>Fe>Al. The soil held more levels of metals than plant parts, but within the permissible limits of concentration. Bark and root accumulated higher levels of trace elements in a magnitude of 10-80 folds than other plant parts. The overall bioaccumulation factor in the sampling sites of Vellar, Pichavaram and Cuddalore was 2.88, 1.42 0.47 respectively. Essential elements accumulate high in mature mangroves forest while non-essential elements accumulate high in the industrially polluted mangroves. Conclusions: The ratio between essential and non-essential elements was found higher in young mangrove forest than that in mature mangrove forest and polluted mangrove areas. Thus, the ratio of accumulation can be used as an index of the growth and pollution status of mangroves.

  16. Kinetic analysis and binding studies of a new recombinant human factor VIIa for treatment of haemophilia.

    Science.gov (United States)

    Grandoni, J; Perret, G; Forier, C

    2017-03-01

    LR769 is a new second-generation recombinant human Factor VIIa (rhFVIIa) developed for haemophilia treatment. We determined enzymatic properties of LR769 and its interaction with antithrombin, tissue factor, platelets and endothelial protein C receptor (EPCR), compared with NovoSevenRT. Kinetic enzyme assays and active site titration were used for enzymatic studies. Surface Plasmon Resonance (SPR) was used for determination of binding constants. Cellular binding was determined for platelets and cultured human umbilical vein endothelial cells (HUVEC). The dissociation constant (Kd ) for activated platelet binding was in the 1 μm range for both products. At saturation, more LR769 than NovoSevenRT was bound to the platelets. Binding to HUVEC was 25-50% higher for LR769 than for NovoSevenRT. Protein C, soluble EPCR, and anti-EPCR antibody all reduced the binding, indicating specificity for EPCR. LR769 was similar to NovoSevenRT in all kinetic assays. Active site titration demonstrated 0.7 mole of active site/mole of protein. The kcat /Km values for activation of FX and FIX with purified recombinant tissue factor and phospholipids were 10.5 s(-1) /0.32 μm and 3.3 s(-1) /0.44 μm respectively. The apparent second-order rate constant for inactivation by human plasma AT was 5.9 ± 0.4 × 10(3) m(-1) s(-1) . The Kd values for binding of LR769 to soluble tissue factor and full-length tissue factor were 8.1 nm and 0.9 nm, respectively, and the Kd for binding to soluble EPCR was 41 nm. Overall, LR769 exhibited characteristics similar to NovoSevenRT, but bound EPCR on HUVEC with somewhat higher affinity than NovoSevenRT. © 2016 LFB Biotechnologies. Haemophilia Published by John Wiley & Sons Ltd.

  17. Evaluating the antibacterial and anticandidal potency of mangrove, Avicennia marina

    Directory of Open Access Journals (Sweden)

    Aseer Manilal

    2016-02-01

    Full Text Available Objective: To evaluate the antibiotic activity of mangrove plant, Avicennia marina (A. marina against human and shrimp pathogens and to delineate bioactive constituents by gas chromatography-mass spectrometer (GC-MS profiling. Methods: The antimicrobial activity of the different polar and non-polar extracts of A. marina was inspected by well diffusion technique against 16 bacterial pathogens and two fungal pathogens. Results: Of the six organic extracts examined, methanolic extract of A. marina fairly repressed the growth of all bacterial and fungal pathogenic strains tested. In general, mangrove extract was more active against the bacterial pathogens while against yeasts, the activity was lesser. The antibiotic activity was attributed to the presence of diverse bioactive secondary metabolites. The chemical profiling of the methanolic extract was performed by GC combined with mass spectrometry. The results of GC-MS showed that the main phytoconstituents were benzeneethanol,4-hydroxy- (RT = 12.173, followed by benzaldehyde,3-methyl- (RT = 6.811. Finally, the GC-MS data evinced that the antimicrobial activity of A. marina was due to the synergistic effect of all constituents or the activity of major constituents. Conclusions: Considering the urgent need of novel antibiotics, the present study brings out a new insight on the exploration of mangroves for antibiotic production in future.

  18. Tres grandes plataformas marinas Mar del Norte

    Directory of Open Access Journals (Sweden)

    Soulas, R.

    1978-03-01

    Full Text Available This article analyzes the different building stages of three large sea platforms. After studying the structure's main characteristics, preparing scale models and calculating ail the elements, platform construction was carried out in three stages: — Dry-dock construction of the hull base. — Installation in the water and finishing the structure. — Immersion and bridge installation. Finally, the necessary operations to move the platform to its definite location were carried out. The structures are formed by a parallelepiped hull of reinforced concrete, divided into compartments by means of vertical orthogonals panels on top of which 2 or 4 piles are placed to support the bridge.

    En este artículo se analizan las diversas etapas en la realización de tres grandes plataformas marinas. Después de un estudio de las características principales de la estructura, ensayos en modelo reducido y cálculo de todos los elementos, se procede a la construcción de las plataformas en tres fases: — Construcción en seco de la base del casco. — Colocación en el agua y acabado de la estructura. — Inmersión y colocación del puente. Por último, se realizan las operaciones necesarias para llevar la plataforma a su ubicación definitiva. Las estructuras están formadas por un casco paralelepipédico de hormigón armado, dividido en compartimientos mediante tabiques verticales ortogonales y sobre el que se han colocado 2 ó 4 pilas que soportan el puente.

  19. A Summary of Eelgrass (Zostera marina) Reproductive Biology with an Emphasis on Seed Biology and Ecology from the Chesapeake Bay Region

    Science.gov (United States)

    2007-12-01

    marina L. seeds. Biologia Marina Mediterranea 7:55-58. Harwell, M. C., and R. J. Orth. 1999. Eelgrass (Zostera marina L.) seed protection for field...Figure 1. Eelgrass (Zostera marina ). ERDC/TN SAV-07-1 December 2007 A Summary of Eelgrass (Zostera marina ) Reproductive Biology with an...has focused on a single species, Zostera marina (eelgrass) (Figure 1). As a result, more is known about the seed ecology of Z. marina than any

  20. Bioaccumulation of Zn and Pb in Avicennia marina (Forsk.) Vierh ...

    African Journals Online (AJOL)

    Accumulation of heavy metals (Zn, Pb) was studied in two mangrove species Avicennia marina (Forsk.)Vierh. and Sonneratia apetala Buch.Ham. under field conditions. Variation in concentration of metals was found in leaf, root and sediment samples of these two species from different localities. Both the species of ...

  1. Genetic variation of mangrove species Avicennia marina in Iran ...

    African Journals Online (AJOL)

    STORAGESEVER

    2008-09-03

    Sep 3, 2008 ... In order to ensure conservation of mangroves, genetic diversity in remaining ... Key words: Avicennia marina, genetic variation, Iran, microsatellite, population. INTRODUCTION. Mangrove forests are a feature of the intertidal zone of ... grammes, and for the conservation of genetic resources. A major ...

  2. Blue carbon stocks in Baltic Sea eelgrass (Zostera marina) meadows

    DEFF Research Database (Denmark)

    Rohr, Maria Emilia; Bostrom, Christoffer; Canal-Vergés, Paula

    2016-01-01

    Although seagrasses cover only a minor fraction of the ocean seafloor, their carbon sink capacity accounts for nearly one-fifth of the total oceanic carbon burial and thus play a critical structural and functional role in many coastal ecosystems. We sampled 10 eelgrass (Zostera marina) meadows in...

  3. Marina Abramović on kohal / Bianka Marran

    Index Scriptorium Estoniae

    Marran, Bianka

    2010-01-01

    Jugoslaavia kunstniku Marina Abramovići performance'itest. Pikemalt tema teosest "The Artist is Present" New Yorgi Moodsa Kunsti Muuseumis 14. märtsist 31. maini 2010. Koostööst saksa fotograafi ja performance'ikunstniku Ulay'ga (kodanikunimi Frank Uwe Laysiepen)

  4. Litter fall and decomposition of mangrove species Avicennia marina ...

    African Journals Online (AJOL)

    Abstract—Litter fall and decomposition of mangrove leaves were compared for different seasons, species (Avicennia marina and Rhizophora mucronata) and sites in southern Mozambique. Mangrove leaf litter fall and decomposition was estimated using small mesh collecting-baskets and litter bags respectively in 2006 and ...

  5. Reproductive anomalies in stenoglossan snails related to pollution from marinas

    Energy Technology Data Exchange (ETDEWEB)

    Smith, B.S.

    1981-02-01

    Over 3090 snails of the dioecious intertidal species Nassarius obsoletus Say were collected from a total of 71 localities. Their reproductive anatomy was examined for a superimposition of male characteristics on to the normal female anatomy, an abnormality called 'imposex'. Imposex was rated numerically in terms of the fraction of the population affected and the intensity of expression in bearer snails. An initial survey of 22 localities in Fairfield and Westport, Connecticut, led to the hypothesis that imposex was related to a substance arising from marinas. This was tested at nine pairs of marina and control localities in Long Island Sound, as well as six pairs along a transect ranging from Rhode Island to Georgia. Imposex scores were significantly higher at the marina locality in every pair. Further confirmation was found in a detailed survey of the estuarine harbor at Southport, Connecticut, which showed that adjacent populations could differ in the amount of imposex to the extent that both the snails and the waters they lived in remained separated by natural or man-made barriers. Imposex has been found in populations of N. obsoletus ranging from Damariscotta, Maine, to Savannah, Georgia, and it has been reported from San Francisco Bay, California. Similar anatomical abnormalities have been reported in at least 27 other species of taenioglossan and stenoglossan snails, extending the range to the Atlantic and Mediterranean coast of Europe and the British Isles. Concern is raised regarding the possible existence of another global pollutant with novel effects on marina biota.

  6. Biosystematic studies on Spergularia media and S. marina in the Netherlands II. The morphological of S. marina

    NARCIS (Netherlands)

    Sterk, A.A.

    1969-01-01

    In this second paper on the biosystematics of the Dutch halophilous Spergularia species, the results are reported of a study of the morphological variation of S. marina by means of population samples from all parts of the Dutch area. This study was supplemented by the rearing of plants from seed

  7. Pharmacological concentrations of recombinant factor VIIa restore hemostasis independent of tissue factor in antibody-induced hemophilia mice.

    Science.gov (United States)

    Keshava, S; Sundaram, J; Rajulapati, A; Pendurthi, U R; Rao, L V M

    2016-03-01

    ESSENTIALS: The role of tissue factor (TF) in recombinant factor VIIa (rFVIIa) therapy in hemophilia is unclear. An acquired mouse hemophilia model with very low or normal levels of human TF was used in the study. rFVIIa is equally effective in correcting the bleeding in mice expressing low or normal levels of TF. Pharmacological doses of rFVIIa restore hemostasis in hemophilia independent of TF. Recombinant factor VIIa (rFVIIa) has been used widely for treating hemophilia patients with inhibitory autoantibodies against factor VIII or IX. Its mechanism of action is not entirely known. A majority of in vitro studies suggested that pharmacological concentrations of rFVIIa restore hemostasis in hemophilia in a phospholipid-dependent manner, independent of tissue factor (TF). However, a few studies suggested that a TF-dependent mechanism has a primary role in correction of bleeding by rFVIIa in hemophilia patients. Here, we investigated the potential contribution of TF in rFVIIa-induced hemostasis in hemophilia employing a model system of FVIII antibody-induced hemophilia in TF transgenic mice. Mice expressing low levels of human TF (LTF mice), mice expressing relatively high levels of human TF (HTF mice) and wild-type mice (WT mice) had neutralizing anti-FVIII antibodies administered in order to induce hemophilia in these mice. The mice were then treated with varying concentrations of rFVIIa. rFVIIa-induced hemostasis was evaluated with the saphenous vein bleeding model. Administration of FVIII inhibitory antibodies induced the hemophilic bleeding phenotype in all three genotypes. rFVIIa administration rescued the bleeding phenotype in all three genotypes. No significant differences were observed in rFVIIa-induced correction of bleeding between LTF and HTF mice that had FVIII antibodies administered. Our results provide strong evidence supporting the suggestion that the hemostatic effect of pharmacological doses of rFVIIa stems from a TF-independent mechanism. © 2016

  8. Control of disseminated intravascular coagulation in Klippel-Trenaunay-Weber syndrome using enoxaparin and recombinant activated factor VIIa: a case report

    Directory of Open Access Journals (Sweden)

    Kecskes Susan

    2010-03-01

    Full Text Available Abstract Introduction Vascular malformation is associated with coagulopathies, especially when hemostasis is challenged. Case presentation We present the case of an 11-year-old Hispanic girl with Klippel-Trenaunay-Weber syndrome that developed disseminated intravascular coagulation after minor surgery, which was controlled by blood product transfusions and enoxaparin to address an ongoing consumptive coagulopathy. The patient, however, developed bacteremia and liver trauma that resulted in severe bleeding. To the best of our knowledge, we report here the first known instance of administering recombinant coagulation factor VIIa to control acute bleeding in a patient with Klippel-Trenaunay-Weber syndrome. Conclusions This case illustrates the concept of enoxaparin maintenance to suppress an ongoing consumptive coagulopathy and the use of recombinant coagulation factor VIIa to control its potentially fatal severe bleeding episodes.

  9. Sulfide Intrusion and Detoxification in the Seagrass Zostera marina

    DEFF Research Database (Denmark)

    Hasler-Sheetal, Harald; Holmer, Marianne

    2015-01-01

    Gaseous sulfide intrusion into seagrasses growing in sulfidic sediments causes little or no harm to the plant, indicating the presence of an unknown sulfide tolerance or detoxification mechanism. We assessed such mechanism in the seagrass Zostera marina in the laboratory and in the field with sca......Gaseous sulfide intrusion into seagrasses growing in sulfidic sediments causes little or no harm to the plant, indicating the presence of an unknown sulfide tolerance or detoxification mechanism. We assessed such mechanism in the seagrass Zostera marina in the laboratory and in the field...... of the detoxification occurred in underground tissues, where sulfide intrusion was greatest. Elemental sulfur was a major detoxification compound, precipitating on the inner wall of the aerenchyma of underground tissues. Sulfide was metabolized into thiols and entered the plant sulfur metabolism as well as being stored...

  10. Successful Off-Label Use of Recombinant Factor VIIa and Coil Embolization in an Adolescent with Massive Hemoptysis Due to Invasive Pulmonary Aspergillosis

    Directory of Open Access Journals (Sweden)

    Dilek Gürlek Gökçebay

    2015-03-01

    Full Text Available Invasive fungal infections have turned out to be a significant cause of morbidity and mortality in pediatric patients with malignant disorders. Massive hemoptysis, a rare complication of invasive pulmonary aspergillosis, may threaten the lives of patients, usually during the resolution of neutropenia. In this report, we describe a patient with massive hemoptysis due to invasive pulmonary aspergillosis whose bleeding was controlled successfully with off-label use of recombinant factor VIIa and subsequent coil embolization of the right pulmonary artery.

  11. Phenology of Avicennia marina (Forsk.) Vierh. in a Disjunctly-zoned ...

    African Journals Online (AJOL)

    Avicennia marina in Gazi Bay, Kenya, displays a disjunct zonation pattern across the intertidal zone with a seaward and a landward A. marina fringe. Earlier studies revealed significant differences in its vegetation structure, physiognomy, root system and leaf morphology, which can be attributed to salinity and tidal ...

  12. The influence of a marina canal system on the ecology of the ...

    African Journals Online (AJOL)

    The influence of a marina canal system on the ecology of the Kromme estuary, St Francis Bay. D. Baird, J.F.K. Marais and T. Wooldridge. Department of Zoology, University of Port Elizabeth, Port Elizabeth. The influence of the marina canals on the lower reaches and mouth area of the Kromme estuary was investigated.

  13. Wax and wane of eelgrass Zostera marina and water column silicon levels

    NARCIS (Netherlands)

    Herman, P.M.J.; Hemminga, M.A.; Nienhuis, P.H.; Verschuure, J.M.; Wessel, E.G.J.

    1996-01-01

    Long-term observations in an enclosed estuarine branch in The Netherlands showed a remarkably strong correlation between water column levels of dissolved silicon (DSi) and standing stock of eelgrass Zostera marina. Si levels in the leaves of Z. marina varied between 0.02 and 0.66% of tissue dry

  14. Effects of salinity and nutrient load and their interaction on Zostera marina

    NARCIS (Netherlands)

    Van Katwijk, M.; Schmitz, G.; Gasseling, A.P.; Van Avesaath, P.H.

    1999-01-01

    Generally, seagrass Zostera marina L. distribution in the Wadden Sea and south-west Netherlands is Limited to waters with low to moderate nutrient concentrations. However, it is known that Z. marina also occurs at high nutrient concentrations when growing in low salinity environments. In this study,

  15. Diurnal effects of anoxia on the metabolome of the seagrass Zostera marina

    DEFF Research Database (Denmark)

    Hasler-Sheetal, Harald; Fragner, Lena; Holmer, Marianne

    2015-01-01

    We investigated the response, adaptation and tolerance mechanisms of the temperate seagrass Zostera marina to water column anoxia. We exposed Z. marina to a diurnal light/dark cycle under anoxia and assessed the metabolic response by measuring the metabolome with gas chromatography coupled to mass...

  16. The Phenomenon of the Marina Development to Support the European Model of Economic Development

    Directory of Open Access Journals (Sweden)

    Joanna Kizielewicz

    2013-09-01

    Full Text Available The development of marinas on the seas, rivers, lakes and canals of Europe occurs in two different forms: (1 as a private investment project, and (2 as an urban municipal investment. Both forms of investment and development of marinas satisfy the criteria of entrepreneurship, which is important for each global and European economy. The purpose of this research is explanation of the possibilities for development of marinas and their immediate and distant destination which supports the development of the local economy. The scientific and research achievements were applied and, for the purpose of transparency, a case study showing several marinas and the examples of two basic models of development are presented. Structurally, this research consists of two parts, namely (1 a theoretical part, where a definition of nautical tourism and its classification are presented and (2 the development of specific indicators of marinas in Europe are explored.

  17. Role of commercial harbours and recreational marinas in the spread of non-indigenous fouling species.

    Science.gov (United States)

    Ferrario, Jasmine; Caronni, Sarah; Occhipinti-Ambrogi, Anna; Marchini, Agnese

    2017-09-01

    The role of commercial harbours as sink and source habitats for non-indigenous species (NIS) and the role of recreational boating for their secondary spread were investigated by analysing the fouling community of five Italian harbours and five marinas in the western Mediterranean Sea. It was first hypothesised that NIS assemblages in the recreational marinas were subsets of those occurring in commercial harbours. However, the data did not consistently support this hypothesis: the NIS pools of some marinas significantly diverged from harbours even belonging to the same coastal stretches, including NIS occurring only in marinas. This study confirms harbours as hotspots for marine NIS, but also reveals that numbers of NIS in some marinas is higher than expected, suggesting that recreational vessels effectively facilitate NIS spread. It is recommended that this vector of NIS introduction is taken into account in the future planning of sustainable development of maritime tourism in Europe.

  18. Comparative Biochemical and Functional Studies on a Branded Human Recombinant Factor VIIa and a Biosimilar Equivalent Product.

    Science.gov (United States)

    Sadeghi, Nasiredin; Kahn, Daniel; Syed, Daneyal; Iqbal, Omer; Abro, Schuharazad; Eshraghi, Reza; Hoppensteadt, Debra; Fareed, Jawed

    2014-09-01

    Recombinant factor VIIa (rFVIIa; NovoSeven, Novo Nordisk, Copenhagen, Denmark) is used to control bleeding in patients with hemophilia. A generic version of FVIIa was developed by AryoGen (Tehran, Iran). This study compared the composition and functional activities of AryoSeven and NovoSeven. Each product was compared at equigravimetric (1 mg/mL) stock solution for protein content. The proteomic profile was obtained using surface-enhanced laser desorption ionization mass spectrometry. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis was carried out to determine the protein profile and Western blotting was performed using a polyclonal rabbit antihuman FVIIa antibody. The FVIIa-related antigen was also measured using a commercially available enzyme-linked immunosorbent assay method. Functional assay included the prothrombin time correction in FVII-deficient plasma. The protein content was comparable in 2 products and the mass spectra analysis showed a single peak at 50 kDa in all products. The SDS-PAGE and immunoblotting studies were comparable. Both products exhibited similar coagulant properties in different assays. © The Author(s) 2014.

  19. Exposiciones. Esculturas de Marina Núñez del Prado

    Directory of Open Access Journals (Sweden)

    Eduardo Mendoza Varela

    1961-05-01

    Full Text Available Hace varios años Gabriela Mistral en unas cuantas palabras que han quedado también entre las mejores páginas, expresó lo que significaba la aparición de Marina Núñez del Prado en el arte de Latinoamérica. En efecto, no era solamente profética la voz de la gran poetisa chilena al señalar en la artista boliviana a una de las figuras más nítidas y altas de la plástica continental.

  20. Vegetative characteristics of Avicennia marina on the artificial inlet

    Directory of Open Access Journals (Sweden)

    AKBAR GHASEMI

    2012-10-01

    Full Text Available The purpose of this project is attempt to ecological development of Avicennia marina in hyper saline condition. During 2001 and 2002 a restoration mangrove project " planting A. marina in artificial inlet in Bushehr Province, Iran" was carried out in Agriculture &Natural resources research center of Bushehr province in Sabkha lands. Some the vegetative characteristics of such as height, diameter and crown diameter in two aspects were recorded in 2012. To investigate physico-chemical properties of soil were taken in inside and outside of inlet and were analyzed: salinity, soil acidity, potassium, phosphorus, nitrogen, texture of soil were measured. Both of treatments were analyzed by T test in SAS Software. The result showed that a different significant between inside and outside of Inlet in soil acidity, electrical conductivity, potassium content and sand and clay percent. But were not different significant in N and P. After ten years halophyte plants such as: Salicornia herbacea and Halocnemum strobilaceum and animals such as: mudskippers were observed into this area. Site conditions have an influence on the growth of seedling, which shows a different growth in the outside and inside of artificial inlets.

  1. Sulfide Intrusion and Detoxification in the Seagrass Zostera marina

    Science.gov (United States)

    Hasler-Sheetal, Harald; Holmer, Marianne

    2015-01-01

    Gaseous sulfide intrusion into seagrasses growing in sulfidic sediments causes little or no harm to the plant, indicating the presence of an unknown sulfide tolerance or detoxification mechanism. We assessed such mechanism in the seagrass Zostera marina in the laboratory and in the field with scanning electron microscopy coupled to energy dispersive X-ray spectroscopy, chromatographic and spectrophotometric methods, and stable isotope tracing coupled with a mass balance of sulfur compounds. We found that Z. marina detoxified gaseous sediment-derived sulfide through incorporation and that most of the detoxification occurred in underground tissues, where sulfide intrusion was greatest. Elemental sulfur was a major detoxification compound, precipitating on the inner wall of the aerenchyma of underground tissues. Sulfide was metabolized into thiols and entered the plant sulfur metabolism as well as being stored as sulfate throughout the plant. We conclude that avoidance of sulfide exposure by reoxidation of sulfide in the rhizosphere or aerenchyma and tolerance of sulfide intrusion by incorporation of sulfur in the plant are likely major survival strategies of seagrasses in sulfidic sediments. PMID:26030258

  2. Influence of neighboring plants on shading stress resistance and recovery of eelgrass, Zostera marina L.

    Directory of Open Access Journals (Sweden)

    Camilla Gustafsson

    Full Text Available Stressful environments may enhance the occurrence of facilitative interspecific interactions between plants. In several regions, Zostera marina occurs in mixed assemblages. However, the potential effects of plant diversity on stress responses and stability properties of Z. marina are poorly understood. We investigated the resistance and recovery of Z. marina subjected to shading (1 mo in a field experiment lasting 2.5 mo. We shaded Z. marina planted in mono- and polycultures (Potamogeton perfoliatus, P. pectinatus, P. filiformis in a factorial design (Shading×Richness at 2 m depth. We estimated the resistance and recovery of Z. marina by measuring four response variables. Polyculture Z. marina lost proportionally less biomass than monocultures, thus having a greater resistance to shading. In contrast, after a 1 mo recovery period, monocultures exhibited higher biomass gain, and a faster recovery than polycultures. Our results suggest that plant species richness enhances the resistance of Z. marina through facilitative mechanisms, while the faster recovery in monocultures is possibly due to interspecific competition. Our results highlight the need of a much better understanding of the effects of interspecific interactions on ecosystem processes in mixed seagrass meadows, and the preservation of diverse plant assemblages to maintain ecosystem functioning.

  3. Towards evidence-based emergency medicine: best BETs from the Manchester Royal Infirmary. BET 2: is recombinant factor VIIa beneficial in the management of acute spontaneous intracerebral haemorrhage?

    Science.gov (United States)

    Dewhirst, Edward

    2013-04-01

    A short-cut review was carried out to establish whether recombinant factor VIIa (rFVIIa) improves survival and functional outcome in acute spontaneous intracranial haemorrhage. Ninety-two papers were found using the reported searches, of which two presented the best evidence to answer the clinical question. The author, date and country of publication, patient group studied, study type, relevant outcomes, results and study weaknesses of these best papers are tabulated. It is concluded that current evidence does not support the use of rFVIIa in acute spontaneous intracranial haemorrhage.

  4. Klassiõpetaja peab särama / Ly Melesk, Kairis Kontus, Leida Talts, Viia Hang...[jt.] ; küsitles Anu Mõttus

    Index Scriptorium Estoniae

    2007-01-01

    Vestlusringis on Tallinna Kuristiku Gümnaasiumi klassiõpetajad Viia Hang ja Ly Melesk, Tallinna Ülikooli 5. kursuse üliõpilane ja Tallinna Lepistiku Lasteaed-Algkooli õpetaja Kairis Kontus, Tallinna Ülikooli algõpetuse õppetooli juhataja Leida Talts ning algõpetuse õppetooli pedagoogika ja algõpetuse metoodika õppejõud Mare Müürsepp. Kui hästi on Tallinna Ülikool ja tema eelkäijad suutnud algklassiõpetajaid ette valmistada ja mis neil igapäevatöös toime tulla aitab

  5. Recombinant human factor VIIa-induced alterations in tissue factor and thrombomodulin in patients with advanced liver cirrhosis.

    Science.gov (United States)

    Van Thiel, David H; Farr, Deborah E; Mindikoglu, Ayse L; Todo, Akira; George, Magdalene M

    2005-06-01

    Recombinant human factor VIIa (rhFVIIa) is used to treat hemophilia and occasionally individuals with liver disease. The aim of the present study was to investigate the consequences of rhFVIIa in individuals with advanced liver disease in an attempt to understand the mechanism of action of rhFVIIa in this unique population. Levels of plasma tissue factor, plasminogen activator inhibitor-1, tissue factor pathway inhibitor, fibrin split products, D-dimers and free thrombomodulin were measured following the administration of rhFVIIa in 17 subjects. The results were compared to normal controls. The prothrombin time declined from 20.2 +/- 2.8 s to 14.3 +/- 3.9 s (P < 0.01). No change in the activated partial thromboplastin time occurred. A 15.6% reduction in thrombomodulin was observed (P < 0.05). A mean 75.2% reduction in plasma tissue factor occurred (P < 0.01). Tissue factor pathway inhibitor levels declined to less than the control value (P < 0.05). No changes in plasminogen activator inhibitor-1, fibrin split products or D-dimer levels occurred. These data demonstrate that rhFVIIa administration to individuals with liver disease results in (i) a transient improvement in the prothrombin time; (ii) no change in the activated partial thromboplastin time; and (iii) a marked reduction in the levels of thrombomodulin and tissue factor. These data suggest that rhFVIIa binds tissue factor and enhances tissue factor and thrombomodulin clearance from the circulation. (c) 2005 Blackwell Publishing Asia Pty Ltd.

  6. Sobre el enfoque estocástico de la dinámica de poblaciones marinas

    OpenAIRE

    Eduardo Ferrandis

    2007-01-01

    dinámica de poblaciones marinas, evaluación de recursos marinos, análisis de supervivencia, modelos deEl presente trabajo trata de profundizar y estructurar el fundamento estadístico de la dinámica de poblaciones marinas. El punto de partida es la relación entre los conceptos de mortalidad, supervivencia y distribución del tiempo de vida. Este triángulo constituye un nido de posibilidades que las técnicas de análisis de supervivencia ofrecen a la dinámica de poblaciones marinas. Se citan...

  7. Blue carbon stocks in Baltic Sea eelgrass (Zostera marina) meadows

    DEFF Research Database (Denmark)

    Rohr, Maria Emilia; Bostrom, Christoffer; Canal-Vergés, Paula

    2016-01-01

    storage and carbon sink capacity of eelgrass meadows in Finland and Denmark, were 281 and 1809 EUR ha(-1), respectively. For a more comprehensive picture of seagrass carbon storage capacity, we conclude that future blue carbon studies should, in a more integrative way, investigate the interactions between......Although seagrasses cover only a minor fraction of the ocean seafloor, their carbon sink capacity accounts for nearly one-fifth of the total oceanic carbon burial and thus play a critical structural and functional role in many coastal ecosystems. We sampled 10 eelgrass (Zostera marina) meadows...... in Finland and 10 in Denmark to explore seagrass carbon stocks (C-org stock) and carbon accumulation rates (C-org accumulation) in the Baltic Sea area. The study sites represent a gradient from sheltered to exposed locations in both regions to reflect expected minimum and maximum stocks and accumulation...

  8. Hydroacoustic basis for detection and characterization of eelgrass (Zostera marina)

    Energy Technology Data Exchange (ETDEWEB)

    Sabol, B. [Army Engineer Waterways Experiment Station, Vicksburg, MS (United States); McCarthy, E. [Naval Undersea Warfare Center, Newport, RI (United States); Rocha, K. [EPA, Narragansett, RI (United States)

    1997-06-01

    Understanding the distribution and density of seagrasses is important for a variety of environmental applications. Physical techniques for detection and characterization are labor and cost intensive and provide little insight into spatial distribution. optical-based techniques are limited by water clarity - frequently resulting in systematic underestimation of the extent of seagrasses. Active hydroacoustic techniques have shown the ability to detect seagrasses but the phenomenology behind detection is poorly understood. Laboratory and in-situ hydroacoustic measurements are presented for eelgrass (Zostera marina), a common seagrass in the United States. Based on these data, hydroacoustic approaches for wide area detection and mapping are discussed and several are demonstrated within areas of established eelgrass beds in Narragansett Bay, Rhode Island.

  9. Eelgrass (Zostera marina) food web structure in different environmental settings

    DEFF Research Database (Denmark)

    Thormar, Jonas; Hasler-Sheetal, Harald; Baden, Susanne

    2016-01-01

    -rich site showed a pyramidal biomass distribution of consumers coupled with a more diverse consumer community. The differences in meadow and food web structure of the two seagrass habitats, suggest how physical setting may shape ecosystem response and resilience to anthropogenic pressure. We encourage......his study compares the structure of eelgrass (Zostera marina L.) meadows and associated food webs in two eelgrass habitats in Denmark, differing in exposure, connection to the open sea, nutrient enrichment and water transparency. Meadow structure strongly reflected the environmental conditions...... composition and food web structure also differed markedly between sites with the eutrophicated, enclosed site having higher biomass of consumers and less complex food web. These relationships resulted in a column shaped biomass distribution of the consumers at the eutrophicated site whereas the less nutrient...

  10. Population specific salinity tolerance in eelgrass (Zostera marina)

    DEFF Research Database (Denmark)

    Salo, Tiina Elina; Pedersen, Morten Foldager; Boström, Christoffer

    2014-01-01

    Salinity is one of the main factors impacting distribution of marine plants and sub-optimal salinities may result in increased resource use and decreased production.We studied the performance and salinity tolerance of eelgrass (Zostera marina L.) originating from two regions with different ambient...... salinities: a low saline (salinity 6) and a high saline (salinity 20) population. Plants fromboth populationswere exposed to a series of fixed salinities (2, 4, 6, 9, 12.5, 15, 20 and 25) for 5 weeks. Both plant origin and salinity affected plant performance significantly. Plant production decreasedwith...... decreasing salinity in both populations,while mortality increased in lowsalinity only in plants originating from the high saline population. The better performance of the low saline population in the lowest salinities indicates a horizontal shift in salinity tolerance among populationswith different origin...

  11. Eelgrass (Zostera marina) food web structure in different environmental settings

    DEFF Research Database (Denmark)

    Thormar, Jonas; Hasler-Sheetal, Harald; Baden, Susanne

    2016-01-01

    his study compares the structure of eelgrass (Zostera marina L.) meadows and associated food webs in two eelgrass habitats in Denmark, differing in exposure, connection to the open sea, nutrient enrichment and water transparency. Meadow structure strongly reflected the environmental conditions...... composition and food web structure also differed markedly between sites with the eutrophicated, enclosed site having higher biomass of consumers and less complex food web. These relationships resulted in a column shaped biomass distribution of the consumers at the eutrophicated site whereas the less nutrient......-rich site showed a pyramidal biomass distribution of consumers coupled with a more diverse consumer community. The differences in meadow and food web structure of the two seagrass habitats, suggest how physical setting may shape ecosystem response and resilience to anthropogenic pressure. We encourage...

  12. Marina Caffiero (Hg.: Rubare le anime. Roma: Viella 2008

    Directory of Open Access Journals (Sweden)

    Rita Unfer Lukoschik

    2009-07-01

    Full Text Available In den Akten des päpstlichen Staatsarchivs und der römischen jüdischen Gemeinde finden sich zeitgenössische Zeugnisse für die im päpstlichen Rom vom 16. bis zum 19. Jahrhundert nicht seltene Zwangskonvertierung jüdischer Mädchen und Frauen zum Katholizismus. Eines der wertvollsten Dokumente dieser Konversionspraxis liegt nunmehr in einer kommentierten Neuedition vor: das Tagebuch der 1749 sich erfolgreich der versuchten Zwangskonvertierung widersetzenden 18-jährigen Anna del Monte. In ihrer Einleitung ordnet die Herausgeberin Marina Caffiero dieses außergewöhliche Einzelschicksal in die zeitgleich und parallel laufenden Assimilations- und Emanzipationsprozesse ein, die sich in der mit aufklärerischem Gedankengut ‚infizierten‘ jüdischen Oberschicht Roms im 18. Jahrhundert abzeichneten.Contemporary reports on the forced conversion of Jewish girls and woman to Catholicism, not a rare occurrence in papal Rome from the 16th to the 19th centuries, can be found in the files of the official papal archive and the Roman Jewish community. One of the most valuable documents of this conversion practice has now been published in a new annotation edition: The diary of 18-year-old Anna del Monte, who was able to successfully resist the attempted forced conversion in 1749. In her introduction the editor Marina Caffiero places this extraordinary individual fate within the concurrent and parallel processes of assimilation and emancipation. These emerge in 18th century Rome’s Jewish upper class, which was “infected” with Enlightenment ideas.

  13. Baltoscandali avavad vigurmarss ja Schubert / Marina Steinmo ; interv. Eva Kübar

    Index Scriptorium Estoniae

    Steinmo, Marina

    2008-01-01

    Baltoscandali teatrifestivalil esineva Eesti päritolu Rootsi tantsija ja koreograafi Charlotte Engelkesi klassikalisel muusikal põhinevatest lavastustest "Forellen and Me" ja "Miss Very Wagner" (dramaturg, teksti ja etenduse kontseptsiooni autor on Marina Steinmo)

  14. Võssotski abikaasa Marina Vlady ei suuda kuulata omaaegset Vene laululegendi / Triin Tael

    Index Scriptorium Estoniae

    Tael, Triin

    2008-01-01

    Legendaarsel Vene lauljal, näitlejal ja luuletajal oleks 25. jaanuaril täitunud 70 eluaastat. Tema eraelust, naistest, pikemalt suhetest Iza Võssotskaja ja Marina Vladyga. Lisaks "Võssotski surm varjutas olümpiamängud"

  15. Eelgrass (Zostera marina) Microsatellite DNA Data; Pacific Coast of North America, 2000-2009

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This data set contains genetic information collected from eelgrass (Zostera marina) populations along the Pacific coast of North America from Alaska to Baha...

  16. Restoring Eelgrass (Zostera marina) from Seed: A Comparison of Planting Methods for Large-Scale Projects

    National Research Council Canada - National Science Library

    Orth, Robert; Marion, Scott; Granger, Steven; Traber, Michael

    2008-01-01

    Eelgrass (Zostera marina) seeds are being used in a variety of both small- and large-scale restoration activities and have been successfully used to initiate recovery of eelgrass in the Virginia seaside coastal lagoons...

  17. Algas marinas bentónicas de la Isla Gorgona, costa pacífica colombiana

    National Research Council Canada - National Science Library

    Mónica Murillo Muñoz; Enrique Javier Peña Salamanca

    2014-01-01

    Se presenta el listado de las algas marinas bénticas del Parque Nacional Natural Isla Gorgona, el inventario corresponde a la flora algal asociada a los diferentes ambientes marinos de la isla, a saber...

  18. Assessment of Marinas in the Mediterranean and the Position of Turkey

    Directory of Open Access Journals (Sweden)

    Evrim Pinar Genc

    2006-05-01

    Full Text Available The most important organizations for developing and advertisingthe yacht tourism in a country are the marinas.Yachting tourism, being a part of maritime sector tends to playa part in the tourist activities and provides important resourcesfor the general economy.In this study, the developments in yachting tourism are aplainedby considering the cu"ent status of the marinas in theMedite"anean countries. Basic characteristics of different marinasof the Medite"anean countries will be discussed and alsostatistical figures will be given. The yachting routes and the potentialof Turkey will be analyzed by emphasizing operational,infrastructural and service characteristics. The factors thatshould be taken into consideration and the methods used formarina marketing will be explained. By considering strengths,weaknesses, opportunities and threats, a SWOT analysis will bemade for Turkey marinas. Suggestions will be given fur furtherdevelopment of marina management in Turkey.

  19. Concentrations of Irgarol in selected marinas of Oahu, Hawaii and effects on settlement of coral larval.

    Science.gov (United States)

    Knutson, Sean; Downs, Craig A; Richmond, Robert H

    2012-01-01

    This study examined concentrations of Irgarol 1051(®) in selected marinas on the island of Oahu, Hawaii and used laboratory bioassays to assess effects of Irgarol on coral larval settlement. Field surveys of small boat marinas performed in 2006-2007 revealed low concentrations of Irgarol 1051(®), an antifouling paint additive, ranging from non-detected (Oahu but Irgarol was not detected in seawater samples at offshore reefs.

  20. Recombinant Factor VIIa Reduces Bleeding after Blunt Liver Injury in a Pig Model of Dilutional Coagulopathy under Severe Hypothermia.

    Directory of Open Access Journals (Sweden)

    Henri M H Spronk

    Full Text Available Recombinant factor VIIa (rFVIIa is registered for use in haemophilia with inhibitors and other rare bleeding disorders, but has also been used in various other clinical conditions to terminate life-threatening bleeding. Underlying conditions (e.g. coagulopathy and dosing may affect treatment efficacy. The objective of the present study was to evaluate the impact of increasing doses of rFVIIa on blood loss and coagulation assays in haemodiluted and hypothermic pigs undergoing blunt liver injury.A grade III blunt liver injury was induced in 28 pigs after 70% haemodilution and cooling to 32.6-33.4°C. Ten minutes after trauma, animals randomly received placebo or 90, 180 or 360 μg/kg rFVIIa. Global coagulation parameters, thromboelastometry (TEM and plasma thrombin generation (TG were determined at different time points during the observation period of 120 minutes.Total blood loss was significantly lower following 90 μg/kg rFVIIa (1206 [1138-1470] mL relative to placebo (2677 [2337-3068] mL; p<0.05, with no increased effect with higher dose levels of rFVIIa. Following trauma and haemodilution, coagulation was impaired relative to baseline in both TEM and TG analysis. At 60 and 120 minutes after trauma, TEM variables improved in the rFVIIa-treated animals compared with the placebo group. Similarly, rFVIIa improved coagulation kinetics in TG. As was observed with blood loss, no significant effect between different rFVIIa dose levels was found in TEM or TG. Macro- and microscopic post-mortem examination did not reveal any signs of thromboembolic events.Early administration of 90 μg/kg rFVIIa reduced blood loss in pigs undergoing blunt liver injury even after severe haemodilution and hypothermia, with no further effect of higher dose levels. Coagulation assays showed impaired coagulation in coagulopathic animals, with a dose-independent improvement in animals treated with rFVIIa.

  1. Ecosystem Services of Avicennia marina in the Red Sea

    KAUST Repository

    Almahasheer, Hanan

    2016-12-01

    The Red Sea is an arid environment, without riverine inputs, oligotrophic waters and extreme temperature and salinity. Avicennia marina is the dominant vegetation in the shores of the Red Sea. However, little is known about their distribution, dynamics, and services. Therefore, the aim of this Ph.D. was to obtain the basic information needed to evaluate their role in the coastal ecosystems and quantify their services. With that objective we 1) estimated the past and present distribution of mangroves in the Red Sea, 2) investigated the growth, leave production and floration 3) examined the growth limiting factors 4) measured the nutrients and heavy metal dynamics in the leaves and 5) estimated carbon sequestration. We found an increase of about 12% in the last 41 years, which contrasts with global trends of decrease. The extreme conditions in the Red Sea contributed to limit their growth resulting in stunted trees. Hence, we surveyed Central Red Sea mangroves to estimate their node production with an average of 9.59 node y-1 then converted that number into time to have a plastochrone interval of 38 days. As mangroves are taller in the southern Red Sea where both temperature and nutrients are higher than the Central Red Sea, we assessed nutrient status Avicennia marina propagules and naturally growing leaves to find the leaves low in nutrient concentrations (N < 1.5 %, P < 0.09 %, Fe < 0.06) and that nutrients are reabsorbed before shedding the leaves (69%, 72% and 35% for N, P, and Fe respectively). As a result, we conducted a fertilization experiment (N, P, Fe and combinations) to find that iron additions alone led to significant growth responses. Moreover, we estimated their leaf production and used our previous estimates of both the total cover mangrove in the Red Sea along with plastochrone interval to assess their total nutrients flux per year to be 2414 t N, 139 t P and 98 t Fe. We found them to sequester 34 g m-2 y-1, which imply 4590 tons of carbon

  2. Aves marinas de las costas e islas colombianas

    Directory of Open Access Journals (Sweden)

    Dugand Armando

    1947-06-01

    Full Text Available La lista de aves marinas que presento en este artículo se refiere en su mayor parte a especies que varios autores (véase Obras citadas han señalado en las costas e islas marítimas de Colombia o en las aguas extraterritoriales del Mar Caribe y del Océano Pacífico inmediatamente próximas a este país. Los ejemplares examinados que menciono fuera de tales referencias pertenecen casi todos a la colección ornitológica del Instituto de Ciencias Naturales y se señalan con las siglas ICN. Unos pocos son del museo de historia natural del Colegio Biffi, en Barranquilla, a cuyo custodio, el Hermano Hildeberto María, doy las gracias por haberme permitido examinarlos. Los que señalo con las palabras Exped. Askoy, seguidas de un numero (de la serie del American Museum of Natural History, forman parte de una interesante colección que nos envió en 1942 el doctor Robert Cushman Murphy, actual Director del Departamento de Aves del American Museum of Natural History, Nueva York. Estos fueron obtenidos por la expedición oceanográfica que, bajo la dirección del doctor Murphy, realizaron en la goleta "Askoy" varios miembros de aquel museo, acompañados por el Comandante Eduardo Fallon, de la Marina Colombiana, en aguas del Pacifico desde Panamá hasta el Ecuador. La Expedición de la "Askoy", que duró de febrero a mayo de 1941, exploró varias bahías y ensenadas en el litoral del Chocó y del Departamento del Valle, así como las islas de Gorgona y Gorgonilla al norte de la costa de Nariño, y el peñón inhabitado de Malpelo, posesión oceánica colombiana situada a unos 500 kilómetros al occidente de Buenaventura, en la latitud de 3° 59' 07" N. y la longitud de 81° 34' 27" W. de Greenwich, según posición determinada por Murphy (1936, I, p. 319, fig. 49 .

  3. Cellulomonas marina sp. nov., isolated from deep-sea water.

    Science.gov (United States)

    Zhang, Limin; Xi, Lijun; Qiu, Danheng; Song, Lei; Dai, Xin; Ruan, Jisheng; Huang, Ying

    2013-08-01

    A bacterial strain FXJ8.089(T) was isolated from deep-sea water collected from the southwest Indian Ocean (49° 39' E 37° 47' S) at a depth of 2800 m, and its taxonomic position was investigated by a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain FXJ8.089(T) belonged to the genus Cellulomonas and had the highest similarities with Cellulomonas oligotrophica (96.9 %) and Cellulomonas aerilata (96.6 %). It contained MK-9(H4) as the predominant menaquinone. The polar lipids were diphosphatidylglycerol and phosphatidylinositol mannosides. The cell-wall peptidoglycan type was A4β with an interpeptide bridge L-Orn-D-Glu. The cell-wall sugars were glucose, mannose and ribose. The DNA G+C content was 70.3 mol%. The strain also showed a number of physiological and biochemical characteristics that were distinct from the closely related species. Based on phenotypic and genotypic data, strain FXJ8.089(T) (= CGMCC 4.6945(T) = DSM 24960(T)) represents a novel species of the genus Cellulomonas, for which the name Cellulomonas marina sp. nov. is proposed.

  4. ESPONJAS MARINAS: ¿PRODUCCIÓN BIOTECNOLÓGICA SOSTENIBLE?

    Directory of Open Access Journals (Sweden)

    LINA BLANDÓN

    2014-07-01

    Full Text Available El cáncer es una enfermedad con amplia incidencia a nivel mundial. La mayoría de los tumores a través del tiempo se vuelven invasivos y metastásicos y los  múltiples tratamientos usados de manera intensa, facilitan la generación de células con  resistencia a éstos. Por lo anterior, en las últimas décadas, se ha estimulado la búsqueda  de nuevas sustancias con potencial bioactivo para el desarrollo de nuevos fármacos  antineoplásicos, lo cual, ha revelado que los organismos marinos,  principalmente las esponjas, ofrecen una gama amplia de compuestos con diferentes actividades, incluyendo la antiproliferativa, útiles en el desarrollo de drogas antitumorales. En la producción farmacéutica sostenible, es requisito producir gran cantidad de biomasa y evitar la sobreexplotación de las especies utilizadas, por esa razón, se requieren estrategias que permitan la producción in vitro de los metabolitos de interés. En esta revisión, se   muestran los principales intentos de producción biotecnológica utilizados en el desarrollo de fármacos provenientes de esponjas marinas y se revisan los estudios genéticos, moleculares y de ciclo celular, encontrados en la literatura científica.

  5. PENGARUH ELEKTROKINETIK TERHADAP DAYA DUKUNG PONDASI TIANG DI LEMPUNG MARINA

    Directory of Open Access Journals (Sweden)

    Daniel Tjandra

    2006-01-01

    Full Text Available Electrokinetics is one of soft ground improvement methods to improve its bearing capacity. The objective of this research is to investigate the increase of friction and end bearing resistance of an embedded instrumented model pile in marine clay after electrokinetics treatment. The initial geotechnical properties of soil surrounding the pile foundation is obtained by several soil laboratory tests. For pile bearing capacity analysis, friction and end bearing resistance of pile was measured by load cell measuring device, while un-drained shear strength analysis was measured by vane shear test. The result of this research showed that after 24 hours electrokinetics treatment, the un-drained shear strength increased closer to the pile, and the bearing capacity of pile increased 14 times. Abstract in Bahasa Indonesia : Elektrokinetik adalah salah satu metode perbaikan tanah lunak yang diaplikasikan untuk meningkatkan daya dukung tanah lunak. Tujuan dari penelitian ini adalah untuk menyelidiki peningkatan tahanan friksi dan ujung suatu model pondasi tiang di lempung marina setelah dilakukan proses elektrokinetik. Karakteristik tanah pada kondisi mula-mula di sekitar pondasi tiang didapatkan dari beberapa pengujian di laboratorium. Untuk analisa daya dukung tiang, tahanan friksi dan ujung dari pondasi tiang diukur dengan alat pengukur load cell, sedangkan analisa kuat geser undrained diukur dengan pengujian baling-baling. Hasil penelitian menunjukkan bahwa setelah pada tanah dilakukan proses elektrokinetik selama 24 jam, daya dukung dari pondasi tiang meningkat 14 kali dan semakin dekat dengan tiang, kuat geser undrained juga semakin meningkat.

  6. Eelgrass (Zostera marina Food Web Structure in Different Environmental Settings.

    Directory of Open Access Journals (Sweden)

    Jonas Thormar

    Full Text Available This study compares the structure of eelgrass (Zostera marina L. meadows and associated food webs in two eelgrass habitats in Denmark, differing in exposure, connection to the open sea, nutrient enrichment and water transparency. Meadow structure strongly reflected the environmental conditions in each habitat. The eutrophicated, protected site had higher biomass of filamentous algae, lower eelgrass biomass and shoot density, longer and narrower leaves, and higher above to below ground biomass ratio compared to the less nutrient-enriched and more exposed site. The faunal community composition and food web structure also differed markedly between sites with the eutrophicated, enclosed site having higher biomass of consumers and less complex food web. These relationships resulted in a column shaped biomass distribution of the consumers at the eutrophicated site whereas the less nutrient-rich site showed a pyramidal biomass distribution of consumers coupled with a more diverse consumer community. The differences in meadow and food web structure of the two seagrass habitats, suggest how physical setting may shape ecosystem response and resilience to anthropogenic pressure. We encourage larger, replicated studies to further disentangle the effects of different environmental variables on seagrass food web structure.

  7. Eelgrass (Zostera marina) Food Web Structure in Different Environmental Settings.

    Science.gov (United States)

    Thormar, Jonas; Hasler-Sheetal, Harald; Baden, Susanne; Boström, Christoffer; Clausen, Kevin Kuhlmann; Krause-Jensen, Dorte; Olesen, Birgit; Rasmussen, Jonas Ribergaard; Svensson, Carl Johan; Holmer, Marianne

    2016-01-01

    This study compares the structure of eelgrass (Zostera marina L.) meadows and associated food webs in two eelgrass habitats in Denmark, differing in exposure, connection to the open sea, nutrient enrichment and water transparency. Meadow structure strongly reflected the environmental conditions in each habitat. The eutrophicated, protected site had higher biomass of filamentous algae, lower eelgrass biomass and shoot density, longer and narrower leaves, and higher above to below ground biomass ratio compared to the less nutrient-enriched and more exposed site. The faunal community composition and food web structure also differed markedly between sites with the eutrophicated, enclosed site having higher biomass of consumers and less complex food web. These relationships resulted in a column shaped biomass distribution of the consumers at the eutrophicated site whereas the less nutrient-rich site showed a pyramidal biomass distribution of consumers coupled with a more diverse consumer community. The differences in meadow and food web structure of the two seagrass habitats, suggest how physical setting may shape ecosystem response and resilience to anthropogenic pressure. We encourage larger, replicated studies to further disentangle the effects of different environmental variables on seagrass food web structure.

  8. EKSPEKTASI DAN PERSEPSI WISATAWAN TERHADAP KUALITAS PELAYANAN PADA MARINA SRIKANDI TOUR & TRAVEL DI PADANGBAI

    Directory of Open Access Journals (Sweden)

    Made Natha Dwipayana

    2013-12-01

    Full Text Available Differences between expectation and perception of visitor about service quality of a travel agency were the background of this research which is entitled “Visitor’s Perception And Expectation of Service Quality At Marina Srikandi Tour And Travel In Padang Bai. Through this research, a thought to maintain professionalism of travel agency with qualified standards, understand different visitor expectations was a major thing that want to be achieved. Background of problem in this research was divided into two problems, such as (1 what are visitor expectation and perception about service quality at Marina Srikandi Tour and travel? (2 How is the visitor’s satisfaction about service quality which is given by Marina Srikandi Tour and travel? Based on the background of problems above, there were two aims of study in this research, they are: (1 to know visitor expectation and perception about service quality at Marina Srikandi Tour and travel, and (2 to know visitor’s satisfaction about service quality which is given by Marina Srikandi Tour and travel.

  9. Do small marinas drive habitat specific impacts? A case study from Mediterranean Sea.

    Science.gov (United States)

    Di Franco, Antonio; Graziano, Mariagrazia; Franzitta, Giulio; Felline, Serena; Chemello, Renato; Milazzo, Marco

    2011-05-01

    Many human activities add new structures to the marine landscape. Despite the fact that human structures cause some inevitable impacts, surprisingly little information exists on the effects of marina on natural marine assemblages. The aim of this paper is to assess habitat-specific response of benthic sessile organisms of rocky shores in relation to the presence of a small marina. Sampling was carried out at three coastal habitats (midshore, lowshore and subtidal) by means of visual censuses adopting an after-control-impact (ACI) experimental design. It appears that the marina affects the structure and composition of benthic communities of both the midshore and the lowshore. Little effect was evident on shallow subtidal assemblage structure. The results of the present study clearly show habitat-specific responses of coastal benthic assemblages to the presence of infrastructure. Copyright © 2011 Elsevier Ltd. All rights reserved.

  10. El Instituto Hidrográfico de la Marina

    Directory of Open Access Journals (Sweden)

    Pérez Carrillo de Albornoz, Francisco J.

    2002-10-01

    Full Text Available Not available

    Se expone en este artículo la amplia labor que desarrolla el Instituto Hidrográfico de la Marina (IHM y su participación en múltiples programas y proyectos nacionales e internacionales, representando a España y manteniendo relaciones permanentes de colaboración con diversas Organizaciones, Comisiones y Comités de carácter supranacional. Tras una síntesis histórica del IHM, cuyos orígenes en el Padrón Real de 1508 le otorgan la rimacía mundial como Instituto Hidrográfico, se describen detalladamente las actividades que desarrolla el Instituto en las diferentes áreas de su competencia (Hidrografia, Geodesia y Fotogrametría, Cartografía, Navegación, Oceanografía, destacando el intenso y continuado trabajo que realizan sus buques hidrográficos y el proceso de transformación que se está llevando a cabo para implantar las modernas tecnologías en todos estos campos de actuación. Haciendo honor a la cita de que «Europa aprendió a navegar en libros españoles», se recogen también en este artículo las numerosas publicaciones que continúa editando el Instituto, como responsable que es de mantener actualizada la cartografía náutica y sus correspondientes avisos y ayudas a la navegación, para finalizar con una referencia a la labor docente que desarrolla su Escuela de Hidrografía.

  11. Copper speciation survey from UK marinas, harbours and estuaries.

    Science.gov (United States)

    Jones, Bryn; Bolam, Thi

    2007-08-01

    The use of copper in antifouling paints has increased in the UK in the last 20 years as TBT and several other organic biocides have been phased out. To assess the probable impact of copper on estuarine systems a survey was undertaken to measure the different fractions of copper present in the water column at current usage. The different fractions measured were; labile copper, (LCu) considered as both the free copper ions and inorganically bound copper, the total dissolved copper (TDCu) present, and the difference between them taken as the organically bound likely non-toxic copper fraction. The survey considered sites with different levels of boat use, namely marinas, harbours and estuaries, differing physical parameters of suspended and dissolved organic matter, different seasons of the year and different depths in the water column all of which control speciation behaviour. Suspended particulate matter (SPM) values were measured at all sites and increased from West to East coast locations (5.7-34.4 mg/l). Dissolved organic matter (DOM) values ranged from 0.58 to 2.2mg/l C. The total dissolved copper concentrations ranged from 0.30 to 6.68 microg/l, with labile fraction ranging from 0.02 to 2.69 microg/l, and most labile copper concentrations below 1 microg/l. None of the yearly mean copper measurements exceeded the 76/464/EEC EQS of 5 microg/l. Of the 306 measurements, only one dissolved copper value in one season was above 5 microg/l. This ratio of labile to total copper was between 10 and 30%. The results from this survey suggest that if toxicity of copper is due to the labile fraction then using the total dissolved copper concentrations as an indicator of impact overestimate the risk by a factor of four times.

  12. Sexual Recruitment in Zostera marina: Progress toward a Predictive Model.

    Science.gov (United States)

    Furman, Bradley T; Peterson, Bradley J

    2015-01-01

    Ecophysiological stress and physical disturbance are capable of structuring meadows through a combination of direct biomass removal and recruitment limitation; however, predicting these effects at landscape scales has rarely been successful. To model environmental influence on sexual recruitment in perennial Zostera marina, we selected a sub-tidal, light-replete study site with seasonal extremes in temperature and wave energy. During an 8-year observation period, areal coverage increased from 4.8 to 42.7%. Gains were stepwise in pattern, attributable to annual recruitment of patches followed by centrifugal growth and coalescence. Recruitment varied from 13 to 4,894 patches per year. Using a multiple linear regression approach, we examined the association between patch appearance and relative wave energy, atmospheric condition and water temperature. Two models were developed, one appropriate for the dispersal of naked seeds, and another for rafted flowers. Results indicated that both modes of sexual recruitment varied as functions of wind, temperature, rainfall and wave energy, with a regime shift in wind-wave energy corresponding to periods of rapid colonization within our site. Temporal correlations between sexual recruitment and time-lagged climatic summaries highlighted floral induction, seed bank and small patch development as periods of vulnerability. Given global losses in seagrass coverage, regions of recovery and re-colonization will become increasingly important. Lacking landscape-scale process models for seagrass recruitment, temporally explicit statistical approaches presented here could be used to forecast colonization trajectories and to provide managers with real-time estimates of future meadow performance; i.e., when to expect a good year in terms of seagrass expansion. To facilitate use as forecasting tools, we did not use statistical composites or normalized variables as our predictors. This study, therefore, represents a first step toward linking

  13. Sexual Recruitment in Zostera marina: Progress toward a Predictive Model.

    Directory of Open Access Journals (Sweden)

    Bradley T Furman

    Full Text Available Ecophysiological stress and physical disturbance are capable of structuring meadows through a combination of direct biomass removal and recruitment limitation; however, predicting these effects at landscape scales has rarely been successful. To model environmental influence on sexual recruitment in perennial Zostera marina, we selected a sub-tidal, light-replete study site with seasonal extremes in temperature and wave energy. During an 8-year observation period, areal coverage increased from 4.8 to 42.7%. Gains were stepwise in pattern, attributable to annual recruitment of patches followed by centrifugal growth and coalescence. Recruitment varied from 13 to 4,894 patches per year. Using a multiple linear regression approach, we examined the association between patch appearance and relative wave energy, atmospheric condition and water temperature. Two models were developed, one appropriate for the dispersal of naked seeds, and another for rafted flowers. Results indicated that both modes of sexual recruitment varied as functions of wind, temperature, rainfall and wave energy, with a regime shift in wind-wave energy corresponding to periods of rapid colonization within our site. Temporal correlations between sexual recruitment and time-lagged climatic summaries highlighted floral induction, seed bank and small patch development as periods of vulnerability. Given global losses in seagrass coverage, regions of recovery and re-colonization will become increasingly important. Lacking landscape-scale process models for seagrass recruitment, temporally explicit statistical approaches presented here could be used to forecast colonization trajectories and to provide managers with real-time estimates of future meadow performance; i.e., when to expect a good year in terms of seagrass expansion. To facilitate use as forecasting tools, we did not use statistical composites or normalized variables as our predictors. This study, therefore, represents a first

  14. Sexual Recruitment in Zostera marina: Progress toward a Predictive Model

    Science.gov (United States)

    2015-01-01

    Ecophysiological stress and physical disturbance are capable of structuring meadows through a combination of direct biomass removal and recruitment limitation; however, predicting these effects at landscape scales has rarely been successful. To model environmental influence on sexual recruitment in perennial Zostera marina, we selected a sub-tidal, light-replete study site with seasonal extremes in temperature and wave energy. During an 8-year observation period, areal coverage increased from 4.8 to 42.7%. Gains were stepwise in pattern, attributable to annual recruitment of patches followed by centrifugal growth and coalescence. Recruitment varied from 13 to 4,894 patches per year. Using a multiple linear regression approach, we examined the association between patch appearance and relative wave energy, atmospheric condition and water temperature. Two models were developed, one appropriate for the dispersal of naked seeds, and another for rafted flowers. Results indicated that both modes of sexual recruitment varied as functions of wind, temperature, rainfall and wave energy, with a regime shift in wind-wave energy corresponding to periods of rapid colonization within our site. Temporal correlations between sexual recruitment and time-lagged climatic summaries highlighted floral induction, seed bank and small patch development as periods of vulnerability. Given global losses in seagrass coverage, regions of recovery and re-colonization will become increasingly important. Lacking landscape-scale process models for seagrass recruitment, temporally explicit statistical approaches presented here could be used to forecast colonization trajectories and to provide managers with real-time estimates of future meadow performance; i.e., when to expect a good year in terms of seagrass expansion. To facilitate use as forecasting tools, we did not use statistical composites or normalized variables as our predictors. This study, therefore, represents a first step toward linking

  15. New species of Parapharyngodon (Nematoda: Pharyngodonidae) in Rhinella marina (Anura: Bufonidae) from Grenada, West Indies.

    Science.gov (United States)

    Bursey, Charles; Drake, Michael; Cole, Rebecca; Sterner Iii, Mauritz; Pinckney, Rhonda; Zieger, Ulrike

    2013-06-01

    Parapharyngodon grenadaensis n. sp. (Nematoda: Pharyngodonidae) from the large intestine of the cane toad, Rhinella marina, is described and illustrated. Parapharyngodon grenadaensis n. sp. is the 48th species assigned to the genus and the 16th species from the Neotropical region. It differs from other species in the genus by possessing 4 pairs of caudal papillae, an echinate anterior cloacal lip, and a blunt spicule of 67-104 μm. This is only the second report of R. marina harboring a species of Parapharyngodon.

  16. Chasing the Intangible: a Conversation on Theatre, Language, and Artistic Migrations with Irish Playwright Marina Carr

    Directory of Open Access Journals (Sweden)

    Valentina Rapetti

    2014-07-01

    Full Text Available Offally born Marina Carr is amongst the most prolific, influential and internationally renowned Irish playwrights of our times. Since her debut on the avant-garde side of the Dublin theatre scene in the late Eighties, she has had  seventeen plays professionally produced, both in and outside Ireland. Her earlier work is influenced by Samuel Beckett’s Absurdist drama, while in her most mature and recent plays she draws on both classical and Irish mythology, Greek tragedies and Shakespeare’s poetics. In this interview, Marina Carr recalls and discusses some pivotal moments of her upbringing and career; she also speaks about language, landscape, dream

  17. Contrasting oxygen dynamics in the freshwater isoetid Lobelia dortmanna and the marine seagrass Zostera marina

    DEFF Research Database (Denmark)

    Sand-Jensen, Kaj; Pedersen, Ole; Binzer, Thomas

    2005-01-01

    of the sediment, and aquatic plants differ in aerenchymal volume and resistance to O2 diffusion through the plant and across leaf and root surfaces. The hypothesis that the freshwater isoetid Lobelia dortmanna and the marine seagrass Zostera marina should display pronounced contrasts in intra-plant O2 dynamics......-sections of the aerenchyma. The major exchange of O2 across roots of L. dortmanna can be accounted for by small intra-plant resistances to diffusion, larger root than leaf surfaces, and greater radial diffusive resistance of leaves than roots. In contrast, the major O2 exchange across leaves than roots of Z. marina can...

  18. Effects of the polychaetes Arenicola marina and Nereis diversicolor on microbial pyrene mineralization

    DEFF Research Database (Denmark)

    Timmermann, Karen; Banta, Gary T.; Johnsen, Anders R.

    2008-01-01

    . diversicolor and especially A. marina decreased the bioavailability of pyrene in surface sediments compared with that of surface sediments in the non-bioturbated control. In conclusion, these polychaetes enhanced microbial pyrene mineralization significantly and this enhancement seemed to be caused......The effects of 2 polychaetes, Nereis diversicolor and Arenicola marina, on the microbial mineralization of the organic contaminant pyrene, a polycyclic aromatic hydrocarbon (PAH), were followed over 44 d. We also examined whether the effect of the polychaetes was caused by enhanced oxygen supply...

  19. Marina Kaljurand: hoolimata kõigest oli möödunud aasta ikkagi hea aasta / Marina Kaljurand ; interv. Igor Taro

    Index Scriptorium Estoniae

    Kaljurand, Marina, 1962-

    2008-01-01

    Ilmunud ka: Postimees : na russkom jazõke 3. jaan., lk. 5. Postimehe poolt aasta inimeseks valitud Eesti suursaadik Venemaal vastab küsimustele, mis puudutavad hinnangut 2007. aastale, pronkssõduriga seotud sündmuste üleelamist Moskvas, Venemaa poliitikute suhtumist Eestisse, Eesti-Vene suhteid. Kommenteerivad: Urmas Paet, Tiit Matsulevitš, Toomas Hendrik Ilves. Lisa: Marina Kaljurand; Pronkssõduri-sõda

  20. A Budget Impact Model of Hemophilia Bypassing Agent Prophylaxis Relative to Recombinant Factor VIIa On-Demand.

    Science.gov (United States)

    Mehta, Darshan A; Oladapo, Abiola O; Epstein, Joshua D; Novack, Aaron R; Neufeld, Ellis J; Hay, Joel W

    2016-02-01

    Hemophilia patients use factor-clotting concentrates (factor VIII for hemophilia A and factor IX for hemophilia B) for improved blood clotting. These products are used to prevent or stop bleeding episodes. However, some hemophilia patients develop inhibitors (i.e., the patient's immune system develops antibodies against these factor concentrates). Hence, these patients do not respond well to the factor concentrates. A majority of hemophilia patients with inhibitors are managed on-demand with the following bypassing agents: recombinant factor VIIa (rFVIIa) and activated prothrombin complex concentrate (aPCC). The recently published U.S. registries Dosing Observational Study in Hemophilia (DOSE) and Hemostasis and Thrombosis Research Society (HTRS) reported higher rFVIIa on-demand use for bleed management than previously described. To estimate aPCC and rFVIIa prophylaxis costs relative to rFVIIa on-demand treatment cost based on rFVIIa doses reported in U.S. registries. A literature-based cost model was developed assuming a base case on-demand annual bleed rate (ABR) of 28.7 per inhibitor patient, which was taken from a randomized phase 3 clinical trial. The doses for rFVIIa on-demand were taken from the median dose per bleed reported by the DOSE and HTRS registries. Model inputs for aPCC and rFVIIa prophylaxis (i.e., dosing and efficacy) were derived from respective randomized clinical trials. Cost analysis was from the U.S. payer perspective, and only direct drug costs were considered. The drug cost was based on the Medicare Part B 2014 average sale price (ASP). Two-way sensitivity and threshold analyses were performed by simultaneously varying on-demand ABR, prophylaxis efficacy, and unit drug cost. In addition to studying relative costs associated with on-demand and prophylaxis treatments, relative cost per bleeding episode avoided were also calculated for aPCC and rFVIIa prophylaxis treatments. The prophylaxis efficacy reported in the trials were used to

  1. Sinkhole hazard assessment in Lesina Marina area (Apulia, Italy)

    Science.gov (United States)

    Canora, F.; Caporale, F.; D'Angella, A.; Fidelibus, D.; Gutierrez, F.; Pellicani, R.; Spilotro, G.

    2012-04-01

    In "Lesina Marina" area, located in the north-western part of the Apulia region (Italy), near the Adriatic coast, sinkhole phenomena are particularly widespread and constitute a risk for the built-up area. These phenomena are due to the structure of the evaporitic rocks located in the study area and to the groundwater regime, influenced by the presence of a channel that connects the sea to the lagoon. The complex sea-channel-lagoon system produces an inland flow towards the channel modulated by the tide with a variable width according to the rules of the coastal aquifers. Further studies have been carried out in order to clarify the context and the causes of this instability phenomenon. A procedure for the sinkhole susceptibility and hazard assessment has been performed, in order to evaluate the spatial distribution of the most unstable areas and the potential spatio-temporal evolution of the phenomenon. The sinkhole susceptibility model has been created in GIS by assessing the spatial relationship between the sinkhole inventory map and a series of thematic maps relative to instability factors. The thematic layers selected for the study are nine and cover geometrical features of the surface, of the gypsum rockhead and of the incoherent soil cover, groundwater and daily and seasonal groundwater level variations. Daily groundwater variation in a semiconfined coastal aquifer can be related to the permeability and to the void structures of the evaporitic mass. In the years subsequent to 1980, when the first reports of the presence of sinkholes are dated, the evolution of these instabilities in terms of their number and of their increase of extension has been monitored with repeated surveys. These data were used for susceptibility model validation and to define the hazard model. The selected layers revealed to be very useful in describing and mapping the hazard coming from suffusion sinkholes in the study area. The sinkhole hazard assessment is carried out, according to

  2. The cane or marine toad, Rhinella marina (Anura, Bufonidae): two genetically and morphologically distinct species.

    Science.gov (United States)

    Acevedo, Aldemar A; Lampo, Margarita; Cipriani, Roberto

    2016-04-18

    Rhinella marina is a Neotropical toad that has been introduced widely worldwide. Its toxic effects to frog-eating predators threaten the native and domestic fauna of some regions where it has been introduced. Despite previous studies suggesting two genetically distinct cryptic species within R. marina, one east and one west of the Andes, its taxonomic status remained unresolved due to the absence of morphological complementary evidence. For the first time, data from two mitochondrial genes (ND3 and CR) and 23 morphometric landmarks are combined to evaluate the taxonomic status of this species. Our results support the hypothesis of two separate evolutionary lineages within R. marina and demonstrate that these lineages have significantly diverged in skull shape. We identified two distinct morphotypes, one eastern and one Andean western, with no overlapping morphospaces. The geographic pattern of genetic variation was consistent with a stable structured population with no evidence of recent demographic or geographic expansions. The concordance between the observed geographic patterns in morphometric and genic traits calls for the recognition of two species under R. marina name.

  3. Biodynamic modelling and the prediction of accumulated trace metal concentrations in the polychaete Arenicola marina

    Energy Technology Data Exchange (ETDEWEB)

    Casado-Martinez, M. Carmen, E-mail: c.casado-martinez@nhm.ac.u [Department of Zoology, The Natural History Museum, Cromwell Rd, London SW7 5BD (United Kingdom); Smith, Brian D. [Department of Zoology, The Natural History Museum, Cromwell Rd, London SW7 5BD (United Kingdom); DelValls, T. Angel [Unesco UNITWIN Wicop Chair, Department of Physical-Chemistry, University of Cadiz, Poligono Industrial Rio San Pedro s/n, C.P. 11510 Puerto Real (Cadiz) (Spain); Luoma, Samuel N. [John Muir Institute of the Environment, University of California at Davis, Davis, CA 95616 (United States); Rainbow, Philip S. [Department of Zoology, The Natural History Museum, Cromwell Rd, London SW7 5BD (United Kingdom)

    2009-10-15

    The use of biodynamic models to understand metal uptake directly from sediments by deposit-feeding organisms still represents a special challenge. In this study, accumulated concentrations of Cd, Zn and Ag predicted by biodynamic modelling in the lugworm Arenicola marina have been compared to measured concentrations in field populations in several UK estuaries. The biodynamic model predicted accumulated field Cd concentrations remarkably accurately, and predicted bioaccumulated Ag concentrations were in the range of those measured in lugworms collected from the field. For Zn the model showed less but still good comparability, accurately predicting Zn bioaccumulation in A. marina at high sediment concentrations but underestimating accumulated Zn in the worms from sites with low and intermediate levels of Zn sediment contamination. Therefore, it appears that the physiological parameters experimentally derived for A. marina are applicable to the conditions encountered in these environments and that the assumptions made in the model are plausible. - Biodynamic modelling predicts accumulated field concentrations of Ag, Cd and Zn in the deposit-feeding polychaete Arenicola marina.

  4. 78 FR 45061 - Safety Zone; Sister Bay Marina Fest Fireworks and Ski Show, Sister Bay, WI

    Science.gov (United States)

    2013-07-26

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; Sister Bay Marina Fest Fireworks and Ski... intended to restrict vessels from a portion of Sister Bay due to a fireworks display and ski show. This... with the fireworks display and ski show in Sister Bay on August 31, 2013. DATES: This rule is effective...

  5. Physiological responses of Zostera marina and Cymodocea nodosa to light-limitation stress.

    Directory of Open Access Journals (Sweden)

    João Silva

    Full Text Available The effects of light-limitation stress were investigated in natural stands of the seagrasses Zostera marina and Cymodocea nodosa in Ria Formosa coastal lagoon, southern Portugal. Three levels of light attenuation were imposed for 3 weeks in two adjacent meadows (2-3 m depth, each dominated by one species. The response of photosynthesis to light was determined with oxygen electrodes. Chlorophylls and carotenoids were determined by high-pressure liquid chromatography (HPLC. Soluble protein, carbohydrates, malondialdehyde and phenol contents were also analysed. Both species showed evident signs of photoacclimation. Their maximum photosynthetic rates were significantly reduced with shading. Ratios between specific light harvesting carotenoids and the epoxidation state of xanthophyll cycle carotenoids revealed significantly higher light harvesting efficiency of C. nodosa, a competitive advantage in a low light environment. The contents of both soluble sugars and starch were considerably lower in Z. marina plants, particularly in the rhizomes, decreasing even further with shading. The different carbohydrate energy storage strategies found between the two species clearly favour C. nodosa's resilience to light deprivation, a condition enhanced by its intrinsic arrangement of the pigment pool. On the other hand, Z. marina revealed a lower tolerance to light reduction, mostly due to a less plastic arrangement of the pigment pool and lower carbohydrate storage. Our findings indicate that Z. marina is close to a light-mediated ecophysiological threshold in Ria Formosa.

  6. Vongola (Chamelea gallina: valutazione degli effetti dei solidi sospesi in acqua marina nel mollusco bivalve

    Directory of Open Access Journals (Sweden)

    Salvatora Angela Angioni

    2010-03-01

    Full Text Available Lo studio è finalizzato alla valutazione degli effetti dei solidi sospesi in acqua marina nella vongola (Chamelea gallina. L’obiettivo è quello di verificare la possibile correlazione tra le morie di vongole, registrate negli ultimi 5 anni, nelle acque costiere del Mare Adriatico centro-settentrionale e l’aumento della concentrazione dei solidi sospesi. La ricerca è stata effettuata mediante l’implementazione di prove sperimentali a 96 h su esemplari divongola, stabulati in acquari contenenti acqua marina filtrata. Le prove sono state precedute da una fase di adattamento dei molluschi bivalvi della durata di 7 giorni. In questo periodo le vongole sono state alimentate con alga unicellulare marina (Dunaliella tertiolecta. I molluschi sono stati esposti a particelle di solidi sospesi costituite da pool di granuli, di varie dimensioni, di silica gel (SiO2 simili a quelli costituenti il limo, la cui presenza e sospensione in mare aumentano notevolmente in seguito ad abbondanti piogge e forti mareggiate. Lo studio ha verificato come le concentrazioni utilizzate dei solidi sospesi in acqua marina, impiegata nelle prove, abbia provocato un numero di esemplari morti, statisticamente, non significativo.

  7. Differing effects of eelgrass Zostera marina on recruitment and growth of associated blue mussels Mytilus edulis

    NARCIS (Netherlands)

    Reusch, B.H.T.

    1998-01-01

    I studied the effects of habitat structure, provided by an eelgrass Zostera marina canopy, on shell growth rate and recruitment of co-occurring blue mussels Mytilus edulis in the Western Baltic Sea. M. edulis in clumps consisting of 10 and 30 individuals were tagged and placed in unvegetated areas

  8. Complete genome of the marine bacterium Wenzhouxiangella marina KCTC 42284(T).

    Science.gov (United States)

    Lee, Sang-Heon; Choe, Hanna; Kim, Byung Kwon; Nasir, Arshan; Kim, Kyung Mo

    2015-12-01

    Wenzhouxiangella marina is an obligatory aerobic, Gram-negative, non-motile, rod-shaped bacterium that was isolated from the culture broth of marine microalgae, Picochlorum sp. 122. Here we report the 3.67 MB complete genome (65.26 G+C%) of W. marina KCTC 42284(T) encoding 3,016 protein-coding genes, 43 tRNAs and one rRNA operon. The genomic information supports multiple horizontal gene transfer (HGT) events in the history of W. marina, possibly with other marine bacteria co-existing in marine habitats. Evaluation of genomic signatures revealed 19 such HGT-derived genomic islands. Of these, eight were also supported by "genomic context" that refers to the existence of integrases, transposases and tmRNA genes either inside or in near vicinity to the island. The addition of W. marina genome expands the repertoire of marine bacterial genomic diversity, especially because the strain represents the sole genomic resource of a novel taxonomic family in the bacterial order Chromatiales. Copyright © 2015 Elsevier B.V. All rights reserved.

  9. [Adaptability of mangrove Avicennia marina seedlings to simulated tide-inundated times].

    Science.gov (United States)

    Liao, Bao-wen; Qiu, Feng-ying; Zhang, Liu-en; Han, Jing; Guan, Wei

    2010-05-01

    A laboratory test on the effects of differents simulated tide-inundated times with 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24 h x d(-1) on the growth of Avicennia marina seedlings was conducted. The ten growth information indices including chlorophyll, root vigor, growth, biomass and photosynthetic rate were mensurated. The principal components analysis was made combining the ten growth information indices. The 210 d experimental results showed that the chlorophyll, root vigor, growth and biomass would rise first and then fall as the extension of the inundate time; and they changed suddenly at the threshold inundate time 16 h x d(-1). The growth and biomass of Avicennia marina seedlings with more than 16 hours tide-inundated time per day were less than them with no more than 16 hours tide-inundated time per day. The maximum value of stem increment each month, leaf blade increment each month, dry weight of stem, dry weight of root and total biomass were under the 10 hours tide-inundated time per day. It concluded that Avicennia marina seedlings would grow adaptively with less than 16 hours tide-inundated time per day, 8-12 hours of tide-inundated time per day is the most suitable for the growth of Avicennia marina seedlings, while 16 h x d(-1) is a critical tide-inundated time when the plant responded to be obviously inadaptable.

  10. North Atlantic phylogeography and large-scale population differentiation of the seagrass Zostera marina L.

    NARCIS (Netherlands)

    Olsen, JL; Stam, WT; Coyer, JA; Reusch, TBH; Billingham, M; Bostrom, C; Calvert, E; Christie, H; Granger, S; La Lumiere, R; Milchakova, N; Oudot-Le Secq, MP; Procaccini, G; Sanjabi, B; Serrao, E; Veldsink, J; Widdicombe, S; Wyllie-Echeverria, S

    As the most widespread seagrass in temperate waters of the Northern Hemisphere, Zostera marina provides a unique opportunity to investigate the extent to which the historical legacy of the last glacial maximum (LGM18000-10000 years BP) is detectable in modern population genetic structure. We used

  11. Akumulasi Logam Berat Tembaga (Cu) Dan Timbal (Pb) Pada Pohon Avicennia marina Di Hutan Mangrove

    OpenAIRE

    Panjaitan, Grace Yanti

    2009-01-01

    Tumbuhan mangrove mempunyai kecenderungan untuk mengakumulasi logam-logam berat yang terdapat dalam ekosistem tempat tumbuhnya. Kemampuan akumulasi logam berat tersebut berbeda untuk setiap species. Salah satu species tumbuhan mangrove yang mampu mengakumulasi logam berat adalah Avicennia marina. Kajian ini dilakukan di dua lokasi, yaitu Hutan Mangrove Pesisir Belawan dan Hutan Mangrove Jaring Halus. Tujuan penelitian adalah untuk menganalisis kandungan logam berat Cu dan...

  12. Atlantic Cod (Gadus morhua benefits from the availability of seagrass (Zostera marina nursery habitat

    Directory of Open Access Journals (Sweden)

    Richard J. Lilley

    2014-12-01

    Full Text Available The Atlantic cod (Gadus morhua is a species of significant economic and historic importance but infamous for its decline. Apart from overfishing, the causes of this decline and its subsequent lack of recovery remain largely unresolved. Indeed, the degree to which specific habitats are important for this species remains unquantified at the scale of North Atlantic. Here, the literature on the role of eelgrass meadows (Zostera marina as valuable nursery habitat for the Atlantic cod is reviewed and synthesized. Evidence is presented on relative densities of Atlantic cod in shallow water environments and in eelgrass meadows in comparison to alternative habitats. In addition, evidence pertaining to the ’viability gains’ attributed to the use of eelgrass meadows as nursery habitat (growth and survival by juvenile Atlantic cod is analyzed. Although juvenile Atlantic cod use of Z. marina is found to be facultative, when possible, available literatures indicates that they may select Z. marina as a nursery habitat where they are found in high density (average of at least 246 ha−1. From their use of Z. marina habitat the juvenile Atlantic cod receives viability benefits from it, improving their chances of reaching maturation. This paper provides strong evidence that eelgrass meadows are of significant importance to contributing to Atlantic cod stocks.

  13. Diurnal effects of anoxia on the metabolome of the seagrass Zostera marina

    DEFF Research Database (Denmark)

    Hasler-Sheetal, Harald; Holmer, Marianne; Weckwerth, Wolfram

    2014-01-01

    Environmental metabolomics has become interesting in marine ecological studies. One example is the revealing of new insights in stress response of Zostera marina. This is essential to understand how, at which level and to what extend aquatic plants adapt, tolerate and react to environmental stres...

  14. Leaf miner-induced morphological, physiological and molecular changes in mangrove plant Avicennia marina (Forsk.) Vierh.

    Science.gov (United States)

    Chen, Juan; Shen, Zhi-Jun; Lu, Wei-Zhi; Liu, Xiang; Wu, Fei-Hua; Gao, Gui-Feng; Liu, Yi-Ling; Wu, Chun-Sheng; Yan, Chong-Ling; Fan, Hang-Qing; Zhang, Yi-Hui; Zheng, Hai-Lei; Tsai, Chung-Jui

    2017-01-31

    Avicennia marina (Forsk.) Vierh is a widespread mangrove species along the southeast coasts of China. Recently, the outbreak of herbivorous insect, Phyllocnistis citrella Stainton, a leaf miner, have impacted on the growth of A. marina. Little is reported about the responses of A. marina to leaf miner infection at the biochemical, physiological and molecular levels. Here, we reported the responses of A. marina to leaf miner infection from the aspects of leaf structure, photosynthesis, and antioxidant system and miner responsive genes expression. A. marina leaves attacked by the leaf miner exhibited significant decreases in chlorophyll, carbon and nitrogen contents, as well as a decreased photosynthetic rate. Scanning and transmission electron microscopic observations revealed that the leaf miner only invaded the upper epidermis and destroyed the epidermal cell, which lead to the exposure of salt glands. In addition, the chloroplasts of mined leaves (ML) were swollen and the thylakoids degraded. The maximal net photosynthetic rate, stomatal conductance (Gs), carboxylation efficiency (CE), dark respiration (Rd), light respiration (Rp) and quantum yields (AQE) significantly decreased in the ML, whereas the light saturation point (Lsp), light compensation point (Lcp), water loss and CO2 compensation point (Г) increased in the ML. Moreover, chlorophyll fluorescence features also had been changed by leaf miner attacks. Interestingly, higher generation rate of O2ˉ· and lower antioxidant enzyme expression in the mined portion (MP) were found; on the contrary, higher H2O2 level and higher antioxidant enzyme expression in the non-mined portion (NMP) were revealed, implying that the NMP may be able to sense that the leaf miner attacks had happened in the MP of the A. marina leaf via H2O2 signaling. Besides, the protein expression of glutathione S-transferase (GST) and the glutathione (GSH) content were increased in the ML. In addition, insect resistance-related gene

  15. Transcriptome sequencing and analysis of leaf tissue of Avicennia marina using the Illumina platform.

    Science.gov (United States)

    Huang, Jianzi; Lu, Xiang; Zhang, Wanke; Huang, Rongfeng; Chen, Shouyi; Zheng, Yizhi

    2014-01-01

    Avicennia marina is a widely distributed mangrove species that thrives in high-salinity habitats. It plays a significant role in supporting coastal ecosystem and holds unique potential for studying molecular mechanisms underlying ecological adaptation. Despite and sometimes because of its numerous merits, this species is facing increasing pressure of exploitation and deforestation. Both study on adaptation mechanisms and conservation efforts necessitate more genomic resources for A. marina. In this study, we used Illumina sequencing of an A. marina foliar cDNA library to generate a transcriptome dataset for gene and marker discovery. We obtained 40 million high-quality reads and assembled them into 91,125 unigenes with a mean length of 463 bp. These unigenes covered most of the publicly available A. marina Sanger ESTs and greatly extended the repertoire of transcripts for this species. A total of 54,497 and 32,637 unigenes were annotated based on homology to sequences in the NCBI non-redundant and the Swiss-prot protein databases, respectively. Both Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed some transcriptomic signatures of stress adaptation for this halophytic species. We also detected an extraordinary amount of transcripts derived from fungal endophytes and demonstrated the utility of transcriptome sequencing in surveying endophyte diversity without isolating them out of plant tissues. Additionally, we identified 3,423 candidate simple sequence repeats (SSRs) from 3,141 unigenes with a density of one SSR locus every 8.25 kb sequence. Our transcriptomic data will provide valuable resources for ecological, genetic and evolutionary studies in A. marina.

  16. Transcriptome sequencing and analysis of leaf tissue of Avicennia marina using the Illumina platform.

    Directory of Open Access Journals (Sweden)

    Jianzi Huang

    Full Text Available Avicennia marina is a widely distributed mangrove species that thrives in high-salinity habitats. It plays a significant role in supporting coastal ecosystem and holds unique potential for studying molecular mechanisms underlying ecological adaptation. Despite and sometimes because of its numerous merits, this species is facing increasing pressure of exploitation and deforestation. Both study on adaptation mechanisms and conservation efforts necessitate more genomic resources for A. marina. In this study, we used Illumina sequencing of an A. marina foliar cDNA library to generate a transcriptome dataset for gene and marker discovery. We obtained 40 million high-quality reads and assembled them into 91,125 unigenes with a mean length of 463 bp. These unigenes covered most of the publicly available A. marina Sanger ESTs and greatly extended the repertoire of transcripts for this species. A total of 54,497 and 32,637 unigenes were annotated based on homology to sequences in the NCBI non-redundant and the Swiss-prot protein databases, respectively. Both Gene Ontology (GO analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG pathway analysis revealed some transcriptomic signatures of stress adaptation for this halophytic species. We also detected an extraordinary amount of transcripts derived from fungal endophytes and demonstrated the utility of transcriptome sequencing in surveying endophyte diversity without isolating them out of plant tissues. Additionally, we identified 3,423 candidate simple sequence repeats (SSRs from 3,141 unigenes with a density of one SSR locus every 8.25 kb sequence. Our transcriptomic data will provide valuable resources for ecological, genetic and evolutionary studies in A. marina.

  17. PENERAPAN MODEL PEMBELAJARAN NUMBER HEADS TOGETHER (NHT UNTUK MENINGKATKAN HASIL BELAJAR SISWA KELAS VIIA SMP NEGERI 2 TUNTANG PADA MATERI SEGITIGA

    Directory of Open Access Journals (Sweden)

    Era Destiyandani

    2016-12-01

    Full Text Available Penelitian tindakan kelas ini bertujuan untuk meningkatkan hasil belajar dan mendeskripsikan kondisi siswa kelas VIIA SMP Negeri 2 Tuntang pada materi segitiga melalui penerapan model pembelajaran Number Heads Together (NHT. Melalui model pembelajaran NHT, tugas diberikan kepada semua siswa dimana siswa telah diberi nomor berbeda didalam kelompoknya namun masing-masing kelompok menggunakan penomoran yang sama. Pemanggilan nomor siswa secara acak untuk melaporkan hasil diskusi kelompok mendorong setiap siswa secara individu bertanggung jawab atas hasil belajarnya. Instrumen penelitian yang digunakan untuk mengambil data penelitian adalah soal tes dan lembar observasi. Penelitian ini diterapkan pada 31 siswa sebagai subjek dan menggunakan model spiral Kemmis dan McTaggart. Hasil penelitian menunjukkan bahwa persentase ketuntasan siswa untuk materi segitiga tentang mengidentifikasi sifat-sifat segitiga berdasarkan sisi dan sudutnya pada siklus I mencapai 87,1% tuntas dan ketuntasan meningkat menjadi 96,77% pada siklus II dengan materi pembelajaran menghitung keliling dan luas segitiga. Hasil ini menunjukkan bahwa penggunaan model pembelajaran NHT telah meningkatkan penguasaan materi segitiga oleh siswa.

  18. A novel supplemental approach to capturing post-marketing safety information on recombinant factor VIIa in acquired hemophilia: the Acquired Hemophilia Surveillance project

    Directory of Open Access Journals (Sweden)

    Lentz SR

    2014-01-01

    Full Text Available Steven R Lentz,1 Anand Tandra,2 Robert Z Gut,3 David L Cooper31Division of Hematology, Oncology and Blood and Marrow Transplantation, Department of Internal Medicine, University of Iowa, Iowa City, IA, USA; 2Hematology, Indiana Hemophilia and Thrombosis Center, Indianapolis, IN, USA; 3Clinical, Medical, and Regulatory Affairs, Novo Nordisk, Inc, Princeton, NJ, USAAcquired hemophilia (AH is a rare (incidence is 1 per 1.5 million but often severe bleeding disorder characterized by autoantibodies to coagulation factor VIII (FVIII. It is associated with life-threatening bleeding and ∼20% mortality.1,2 Recombinant factor VIIa (rFVIIa; NovoSeven® RT, Novo Nordisk A/S, Bagsværd, Denmark received an indication from the US Food and Drug Administration (FDA in October 2006 for the treatment of bleeding episodes and the prevention of bleeding in surgical interventions or invasive procedures in patients with AH.3View original paper by Collins and colleagues.

  19. EFECTO ANTIMITÓTICO IN VITRO EN EL EXTRACTO METANÓLICO DE MACROALGAS MARINAS DE LA COSTA CARIBE COLOMBIANA

    National Research Council Canada - National Science Library

    OSPINA G, Sandra P; LÓPEZ O, Juan B; MÁRQUEZ F, María E

    2007-01-01

    En la búsqueda de nuevas posibilidades quimioterapéuticas para el tratamiento del cáncer, se han reportado numerosos hallazgos de actividad antitumoral en extractos derivados de diversas especies de macroalgas marinas...

  20. Effects of coexistence between the blue mussel Mytilus edulis and eelgrass Zostera marina on sediment biogeochemistry and plant performance

    DEFF Research Database (Denmark)

    Vinther, H.F.; Norling, P.; Kristensen, Per Sand

    2012-01-01

    The habitat-modifying suspension-feeding mussel, Mytilus edulis, may have facilitating or inhibiting effects on seagrass meadows depending on the environmental conditions. We investigated the effects of M. edulis on sediment biogeochemistry in Zostera marina meadows under eutrophic conditions...... in Flensborg fjord, Denmark. Sediment and plant samples were collected at ten stations; five with Z. marina (Eelgrass) and five with Z. marina and M. edulis (Mixed) and at two unvegetated stations; one with mussels (Mussel) and one with sand (Sand). The Mixed sediment was enriched in fine particles (2-3 times...... significantly reduced at Mixed stations suggesting inhibiting effect of M. edulis on Z. marina. Negative correlations between eelgrass measures and sediment sulphide at Mixed stations indicate that presence of mussels increase sulphide invasion in the plants. A survey of 318 stations in Danish fjords suggests...

  1. A comparative evaluation of biological activities and bioactive compounds of the seagrasses Zostera marina and Zostera noltei from southern Portugal.

    Science.gov (United States)

    Custódio, Luísa; Laukaityte, Simona; Engelen, Aschwin H; Rodrigues, Maria João; Pereira, Hugo; Vizetto-Duarte, Catarina; Barreira, Luísa; Rodríguez, Hortensia; Alberício, Fernando; Varela, João

    2016-01-01

    This work assessed the antioxidant potential, acetylcholinesterase (AChE) inhibition and the in vitro cytotoxic activity of extracts of the seagrasses Zostera marina and Zostera noltei collected from southern Portugal. The total phenolic contents (TPCs), the rosmarinic acid (RA) concentration (HPLC/DAD) and the fatty acid (FA) profile (GC/MS) are also described. Z. marina had the highest TPC, radical scavenging activity against DPPH radicals and copper chelating activity. Z. noltei had metal chelation capacity to copper and iron ions. None of the species was able to inhibit AChE. Both seagrasses had high levels of polyunsaturated FAs. Z. marina significantly and selectively reduced the viability of tumorous neuronal cells. Z. noltei was highly toxic for the three cell lines tested and was selective against hepatocarcinoma cells at the concentration of 100 μg/mL. RA was the main compound identified in Z. marina, but not in Z. noltei.

  2. ASOCIACIONES ENTRE AVES MARINAS Y SOTALIA GUIANENSIS EN EL SUR DEL GOLFO DE VENEZUELA

    Directory of Open Access Journals (Sweden)

    NÍNIVE ESPINOZA-RODRÍGUEZ

    2015-01-01

    Full Text Available Las asociaciones entre aves marinas y mamíferos marinos es un evento común en todos los mares y océanos del mundo. Muchos autores han denominado estas asociaciones como relaciones comensalistas, oportunistas o parasitarias, según el efecto que dicha interacción resulte sobre una o ambas especies relacionadas. Con la finalidad de describir la existencia de agrupaciones entre Sotalia guianensis y aves marinas en la porción sur del Golfo de Venezuela, desde junio 2011 a junio 2012 se realizaron observaciones en plataformas móviles de grupos de este cetáceo y aves marinas, utilizando el protocolo "group-follow" bajo la metodología de "Ad libitum sampling". Todos los avistamientos fueron georreferenciados, realizándose anotaciones de la ocurrencia o no-asociación con aves marinas, de igual forma, se registró la especie y el número de aves presentes al momento de la interacción. Se realizaron 721 avistamientos, de los cuales en 197 eventos se registró asociación entre aves marinas y Sotalia guianensis. Las especies de aves marinas residentes que presentaron mayor frecuencia en eventos de asociación con S. guianensis fueron: Fregata magnificens (49%; n=98, Phalacrocorax brasilianus (29,5%; n=59 y Pelecanus occidentalis (22,5%; n=45; siendo Thalasseus maxima (71%; n=142 la única especie migratoria. Durante las observaciones realizadas en el período de muestreo se notó la presencia de una especie de golondrina (Riparia riparia en un solo evento de agrupación con Sotalia guianensis. Dichas asociaciones aves-delfines, sólo fueron observadas cuando notables congregaciones de peces fueron registrados, donde el o los grupos de delfines realizaban alguna actividad con grandes movimientos de agua, lo que pudiese permitir a las aves realizar un menor gasto energético en la ubicación y la captura de la presa.

  3. Entamoeba marina n. sp.; a New Species of Entamoeba Isolated from Tidal Flat Sediment of Iriomote Island, Okinawa, Japan.

    Science.gov (United States)

    Shiratori, Takashi; Ishida, Ken-Ichiro

    2016-05-01

    The genus Entamoeba includes anaerobic lobose amoebae, most of which are parasites of various vertebrates and invertebrates. We report a new Entamoeba species, E. marina n. sp. that was isolated from a sample of tidal flat sediment collected at Iriomote Island, Okinawa, Japan. Trophozoites of E. marina were 12.8-32.1 μm in length and 6.8-15.9 μm in width, whereas the cysts were 8.9-15.8 μm in diam. and contained four nuclei. The E. marina cells contained a rounded nucleus with a small centric karyosome and uniformly arranged peripheral chromatin. Although E. marina is morphologically indistinguishable from other tetranucleated cyst-forming Entamoeba species, E. marina can be distinguished from them based on the combination of molecular phylogenetic analyses using SSU rDNA gene and the difference of collection sites. Therefore, we propose E. marina as a new species of the genus Entamoeba. © 2015 The Author(s) Journal of Eukaryotic Microbiology © 2015 International Society of Protistologists.

  4. 'A Moça Tecelã' de Marina Colasanti

    Directory of Open Access Journals (Sweden)

    Dayana da Silva Rhoden

    2012-04-01

    Full Text Available No presente trabalho, estudaremos o conto A Moça Tecelã de Marina Colasanti. Mostraremos a relação que a autora se utiliza para criar uma nova vestimenta a esta narrativa. Dessa maneira analisaremos através da retomada da mitologia, mostrando a estratégia do arquétipo representando a figura da mulher e a volta ao passado para evidenciar a mulher da atualidade. Encontramos nesta narrativa elementos que retomam a mitologia, evidenciando assim a tese que os mitos e os clássicos são constantes e primordiais nos tempos atuais. Palavras-chave: letras; Marina Colasanti; mitologia; mulher.

  5. Removal Efficiency of Different Gemini Surfactants and Related Modified Clay toChattonella marina.

    Science.gov (United States)

    Wang, Wen-Wen; Yan, Xin-Ya; Li, Yun-Hui; Yu, De-Ren; Li, Hong-Ye; Yang, Wei-Dong; Liu, Jie-Sheng

    2017-11-01

      To obtain new modified clays with excellent algae removal efficiency, three gemini surfactants including ethylene bis (dodecyl dimethyl ammonium chloride), ethylene bis (octadecyl dimethyl ammonium chloride) and ethylene bis (dodecyl dimethyl ammonium bromide) (EDAB), and a poly quaternary ammonium salt, poly dimethyl diallyl ammonium chloride, were screened with Chattonella marina. The four chemicals all exhibited high removal efficiencies against C. marina, with EDAB achieving the highest. A series of organ-clays with different ratios of EDAB were prepared, and the associated removal efficiencies were evaluated. The removal efficiencies of the organ-clays were improved by the EDAB intercalation and the organ-clay with 15% EDAB had the highest removal efficiency. The LC50 of EDAB intercalated clay for zebrafish and shrimp was much higher than the values of intercalated clay required to obtain a desirable removal efficiency of algae. Taken together, EDAB intercalated clay might be a potential alternative to control harmful algal blooms (HABs).

  6. [Impacts of digging Phascolosoma esculenta on the growth of mangrove Avicennia marina seedlings: a simulation study].

    Science.gov (United States)

    He, Qin-Fei; Fan, Hang-Qing; Mo, Zhu-Cheng; Wang, Xin; Shen, Wen-Hui

    2012-04-01

    Digging Phascolosoma esculenta to increase income is one of the main causes leading to the degradation of China mangroves. In order to understand the impact mechanisms of digging P. esculenta on the mangrove growth and to select indicators to evaluate the mangrove health, a simulative study was conducted to clarify the impacts of digging depth, radian, and frequency on the growth of 1-year old Avicennia marina seedlings, with the indices seedling height, basal diameter, single leaf area, specific leaf area, total biomass, and dead root dry mass measured. The results showed that digging activities decreased the increment of seedling height and basal diameter, single leaf area, specific leaf area, and total biomass significantly, and increased the dead root dry mass markedly. Digging depth and radian had obvious effects on the growth of A. marina seedlings, but digging frequency had minor effects. When the digging depth was radian was 5 cm, the damage was quite serious.

  7. Time-eternity as the main concept of Marina Tsvetaeva’s style

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    Bezrukov Andrei Nikolayevich

    2017-12-01

    Full Text Available The article presents the analysis of the lyric poetry by M. Tsvetaeva. The presence of the concept ‘time-eternity’ in her work is a feature of the author's individual style. Marina Tsvetaeva has a unique ability, i.e. mastery of a language that extends the semantic potential of the work. This concept forms the reality, focuses on the poetic aesthetics and deep personal experience. The essence of the analysis is to show how the concept enables fate of the whole. More attention is paid on a series of texts that merge the concept ‘time-eternity’ into some point. The article focuses on the peculiarity of Marina Tsvetaeva’s style. The analysis of her style and language gave an opportunity to see a variety of elements combined in a single art construct. In conclusion, her individual style of writing Tsvetaeva integrates into a particular concept on phonetic, lexical, and syntactic levels.

  8. Seasonal acclimation in metabolism reduces light requirements of eelgrass (Zostera marina)

    DEFF Research Database (Denmark)

    Stæhr, Peter A.; Borum, Jens

    2011-01-01

    rates, especially so for cold acclimated plants in February (36% higher Q10-values). These changes were accompanied by a reduction in chlorophyll a and nitrogen concentrations in leaves by 35% and 60% respectively from February to August. The critical light requirement (EC) of Z. marina to maintain...... a positive carbon balance increased exponentially with increasing temperature but less so for summer-acclimated than for winter-acclimated plants. However, combining EC vs temperature models for whole-plants with data on daily light availability showed that seasonal acclimation in metabolism increased......We investigated the ability of eelgrass (Zostera marina) to adjust light requirements to seasonal changes in temperature, light and nutrient conditions through changes in metabolism, pigment and nutrient content. In agreement with expectations we found that rates of respiration and light saturated...

  9. The effect of wind direction and building surroundings on a marina bay in the Black Sea

    Science.gov (United States)

    Katona, Cosmin; Safta, Carmen Anca

    2017-01-01

    The wind effect has usually a major importance in the marina bay. These environmental sites are an interplay between tourist and commercial activities, requiring a high-detailed and definition studies of the dynamic fluid in the harbor. Computational Fluid Dynamics (CFD) has been used elaborately in urban surroundings research. However, most CFD studies were performed for harbors for only a confined number of wind directions and/or without considering the building surroundings effects. This paper presents the results of different simulations based on various wind flows and the CFD simulation of coupled urban wind flow and general wind directions upon a semi-closed area. Thus the importance of wind effects on the evaluation of the marina bay will be pointed out to achieve a safe and secure mooring at the berth and eventually a good potential of renewable energy for an impending green harbor.

  10. Mild Maternal Iron Deficiency Anemia Induces Hearing Impairment Associated with Reduction of Ribbon Synapse Density and Dysregulation of VGLUT3, Myosin VIIa, and Prestin Expression in Young Guinea Pigs.

    Science.gov (United States)

    Yu, Fei; Hao, Shuai; Yang, Bo; Zhao, Yue; Zhang, Wenyue; Yang, Jun

    2016-05-01

    Mild maternal iron deficiency anemia (IDA) adversely affects the development of cochlear hair cells of the young offspring, but the mechanisms underlying the association are incompletely understood. The aim of this study was to evaluate whether mild maternal IDA in guinea pigs could interrupt inner hair cell (IHC) ribbon synapse density and outer hair cell motility of the offspring. Here, we established a dietary restriction model that allows us to study quantitative changes in the number of IHC ribbon synapses and hearing impairment in response to mild maternal IDA in young guinea pig. The offspring were weaned on postnatal day (PND) 9 and then were given the iron-sufficient diet. On PND 24, pups were examined the hearing function by auditory brainstem response (ABR) and distortion product otoacoustic emission (DPOAE) measurements. Then, the cochleae were harvested for assessment of the number of IHC ribbon synapses by immunofluorescence, the morphology of cochlear hair cells, and spiral ganglion cells (SGCs) by scanning electron microscope and hematoxylin-eosin staining, the location, and expression of vesicular glutamate transporter (VGLUT) 3, myosin VIIa, and prestin by immunofluorescence and blotting. Here, we show that mild maternal IDA in guinea pigs induced elevated ABR threshold shifts, declined DPOAE level shifts, and reduced the number of ribbon synapses, impaired the morphology of cochlear hair cells and SGCs in offsprings. In addition, downregulation of VGLUT3 and myosin VIIa, and upregulation of prestin were observed in the cochlea of offsprings from mild maternal IDA in guinea pigs. These data indicate that mild maternal IDA in guinea pigs induced hearing impairment in offsprings, and this deficit may be attributed to the reduction of ribbon synapse density and dysregulation of VGLUT3, myosin VIIa, and prestin.

  11. Biology of the Marine Heterotrophic Dinoflagellate Oxyrrhis marina: Current Status and Future Directions

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    Sheng Liu

    2013-10-01

    Full Text Available Heterotrophic dinoflagellates are prevalent protists in marine environments, which play an important role in the carbon cycling and energy flow in the marine planktonic community. Oxyrrhis marina (Dinophyceae, a widespread heterotrophic dinoflagellate, is a model species used for a broad range of ecological, biogeographic, and evolutionary studies. Despite the increasing research effort on this species, there lacks a synthesis of the existing data and a coherent picture of this organism. Here we reviewed the literature to provide an overview of what is known regarding the biology of O. marina, and identify areas where further studies are needed. As an early branch of the dinoflagellate lineage, O. marina shares similarity with typical dinoflagellates in permanent condensed chromosomes, less abundant nucleosome proteins compared to other eukaryotes, multiple gene copies, the occurrence of trans-splicing in nucleus-encoded mRNAs, highly fragmented mitochondrial genome, and disuse of ATG as a start codon for mitochondrial genes. On the other hand, O. marina also exhibits some distinct cytological features (e.g., different flagellar structure, absence of girdle and sulcus or pustules, use of intranuclear spindle in mitosis, presence of nuclear plaque, and absence of birefringent periodic banded chromosomal structure and genetic features (e.g., a single histone-like DNA-associated protein, cob-cox3 gene fusion, 5′ oligo-U cap in the mitochondrial transcripts of protein-coding genes, the absence of mRNA editing, the presence of stop codon in the fused cob-cox3 mRNA produced by post-transcriptional oligoadenylation, and vestigial plastid genes. The best-studied biology of this dinoflagellate is probably the prey and predators types, which include a wide range of organisms. On the other hand, the abundance of this species in the natural waters and its controlling factors, genome organization and gene expression regulation that underlie the unusual

  12. Impact of polychaetes (Nereis spp. and Arenicola marina) on carbon biogeochemistry in coastal marine sediments†

    OpenAIRE

    Kristensen Erik

    2001-01-01

    Known effects of bioturbation by common polychaetes (Nereis spp. and Arenicola marina) in Northern European coastal waters on sediment carbon diagenesis is summarized and assessed. The physical impact of irrigation and reworking activity of the involved polychaete species is evaluated and related to their basic biology. Based on past and present experimental work, it is concluded that effects of bioturbation on carbon diagenesis from manipulated laboratory experiments cannot be directly extr...

  13. Rhizosphere microbiome metagenomics of gray mangroves (Avicennia marina) in the Red Sea

    KAUST Repository

    Alzubaidy, Hanin S.

    2015-11-10

    Mangroves are unique, and endangered, coastal ecosystems that play a vital role in the tropical and subtropical environments. A comprehensive description of the microbial communities in these ecosystems is currently lacking, and additional studies are required to have a complete understanding of the functioning and resilience of mangroves worldwide. In this work, we carried out a metagenomic study by comparing the microbial community of mangrove sediment with the rhizosphere microbiome of Avicennia marina, in northern Red Sea mangroves, along the coast of Saudi Arabia. Our results revealed that rhizosphere samples presented similar profiles at the taxonomic and functional levels and differentiated from the microbiome of bulk soil controls. Overall, samples showed predominance by Proteobacteria, Bacteroidetes and Firmicutes, with high abundance of sulfate reducers and methanogens, although specific groups were selectively enriched in the rhizosphere. Functional analysis showed significant enrichment in ‘metabolism of aromatic compounds’, ‘mobile genetic elements’, ‘potassium metabolism’ and ‘pathways that utilize osmolytes’ in the rhizosphere microbiomes. To our knowledge, this is the first metagenomic study on the microbiome of mangroves in the Red Sea, and the first application of unbiased 454-pyrosequencing to study the rhizosphere microbiome associated with A. marina. Our results provide the first insights into the range of functions and microbial diversity in the rhizosphere and soil sediments of gray mangrove (A. marina) in the Red Sea.

  14. Utjecaj propil galata i ekstrakta ružmarina na oksidacijsku stabilnost smjese biljnih ulja

    Directory of Open Access Journals (Sweden)

    T. Moslavac

    2010-01-01

    Full Text Available U ovom radu istraživan je utjecaj sintetskog antioksidansa propil galata (0,01 % i prirodnog ekstrakta ružmarina Oxy´Less®CS (0,1 % i StabilEnhance®OSR (0,1 % na oksidacijsku stabilnost smjese suncokretovog ulja i ostalih vrsta biljnih ulja (50:50. Istraživana je smjesa suncokretovog ulja (50 % i ostalih vrsta biljnih ulja (50 %: sojino ulje, ulje kukuruzne klice, repičino ulje i ulje koštica grožđa. Oksidacijska stabilnost različitih smjesa biljnih ulja, sa i bez dodanog sintetskog i prirodnog antioksidansa, ispitivana je primjenom Rancimat testa. Rezultat oksidacije ulja izražen je sa indukcijskim periodom (IP na bazi kojih je računat i zaštitni faktor (PF. Stabilnost smjese ulja proporcionalna je indukcijskom periodu. Prirodni antioksidans ekstrakt ružmarina Oxy´Less®CS efikasnije povećava oksidacijsku stabilnost smjese suncokretovog ulja i ostalih ispitivanih ulja (50:50 u odnosu na StabilEnhance®OSR i propil galat. Rezultat istraživanja pokazuje da visoku antioksidacijsku aktivnost, mjerenu kao indukcijski period i zaštitni faktor, ima ekstrakt ružmarina Oxy´Less®CS u svim smjesama ulja. Sintetski antioksidans propil galat efikasnije povećava oksidacijsku stabilnost smjese ulja u odnosu na StabilEnhance®OSR.

  15. Characterisation of esterases as potential biomarkers of pesticide exposure in the lugworm Arenicola marina (Annelida: Polychaeta)

    Energy Technology Data Exchange (ETDEWEB)

    Hannam, Marie L. [Ecotoxicology and Stress Biology Research Centre, School of Biological Sciences, University of Plymouth, Drake Circus, Plymouth, Devon, PL4 8AA (United Kingdom)], E-mail: marie.hannam@plymouth.ac.uk; Hagger, Josephine A.; Jones, Malcolm B.; Galloway, Tamara S. [Ecotoxicology and Stress Biology Research Centre, School of Biological Sciences, University of Plymouth, Drake Circus, Plymouth, Devon, PL4 8AA (United Kingdom)

    2008-03-15

    Here, we identify and characterise cholinesterase (ChE) and carboxylesterase (CbE) activities in the body tissues of the sediment dwelling worm Arenicola marina. Exposure to the organophosphorus pesticide azamethiphos yielded an in vitro IC{sub 50} of 5 {mu}g l{sup -1} for propionylcholinesterase (PChE). PChE was significantly inhibited in vivo after a 10 day exposure to 100 {mu}g l{sup -1} azamethiphos, equivalent to the recommended aquatic application rate (ANOVA; F = 2.75, P = 0.033). To determine sensitivity to environmental conditions, A. marina were exposed for 10 days to field collected sediments. PChE activity was significantly lower in worms exposed to sediments from an estuary classified to be at high risk from point source pollution by the UK Environment Agency (ANOVA; F = 15.33, P < 0.001). Whilst causality cannot be directly attributed from these latter exposures, they provide an important illustration of the potential utility of esterase activity as a biomarker of environmental quality in this ecologically relevant sentinel species. - This paper provides a preliminary characterisation of esterase enzyme activities in the tissues and body fluids of the sediment dwelling worm Arenicola marina and explores their potential use as biomarkers of organophosphorus pesticide exposure in the marine environment.

  16. Modulation of endothelial cell inflammatory integrins and stress markers with rh-factor VIIa in patients with advanced chronic hepatitis C.

    Science.gov (United States)

    Van Thiel, D H; Anantharaju, A; Mindikoglu, A L; Shah, N; Leone, N; Bejna, J; Tarasuk, G; Todo, A; Mobarhan, S; George, M

    2003-07-01

    Individuals with chronic hepatitis C (CHC) progress to cirrhosis and hepatic cancer. Individuals with advanced CHC are coagulopathic and can manifest fibrinolysis. The coagulopathy is a consequence of hepatocytic dysfunction. The fibrinolysis represents a response to local endothelial cell injury, and is of a low-grade. Based upon this hypothesis, the effect of the infusion of recombinant human factor VIIa (rh-FVIIa) on endothelial cell inflammatory integrins and measures of endothelial stress were determined in 17 individuals with advanced CHC. Immediately prior to the infusion of rh-FVIIa, the plasma levels of tissue factor (TF), Thrombomodulin (TM), human soluble ICAM-1 (hs-ICAM-1), human soluble VCAM-1 (hs-VCAM-1), human soluble L-Selectin (hs-L-Selectin), the prothrombin time and the activated partial thromboplastin time were determined. The same parameters were assayed at 5, 10, 30, 120, 240 and 360 min after infusion. TF and TM levels were very high at baseline consistent with a vascular endothelial stress response. Similarly hs-ICAM-1, hs-VCAM-1 as well as L-Selectin levels were increased. Thirty minutes after the infusion, a marked reduction in ICAM-1 and VCAM-1 and to a lesser degree L-Selectin levels was observed. This reduction persisted for 360 min. No change in measures of fibrinolysis [plasminogen activator inhibitor-1 (PAI-1), total tissue factor pathway inhibitor (t-TFPI), activated tissue factor pathway inhibitor (TFPIa), d-dimers (DD), FSP and fibrinogen levels] occurred. In addition, no change in plasma Annexin-V was observed. Based upon these data it can be concluded that: (1) rh-FVIIa corrects the coagulopathy seen in advanced CHC; (2) reduces endothelial cell injury and/or stress as evidenced by the TF, TM, hs-ICAM-1 and hs-VCAM-1 levels in plasma; (3) these changes in coagulation occurred without inducing a propagated vascular thrombosis.

  17. Recombinant Activated Factor VIIa (rFVIIa) Treatment in Very-Low-Birth-Weight (VLBW) Premature Infants with Acute Pulmonary Hemorrhage: A Single-Center, Retrospective Study.

    Science.gov (United States)

    Cosar, Hese; Isik, Halil; Cakır, Salih Cagrı; Yar, Nese; Goksen, Bulent; Tokbay, Hakan; Kertmen, Hasan; Erdoğan, Nihal; Durak, Ikbal

    2017-02-01

    We aimed to evaluate the efficacy of intravenous administration of recombinant activated factor VIIa (rFVIIa) for acute pulmonary hemorrhage treatment in very-low-birth-weight (VLBW) premature infants. This study was carried out retrospectively in premature infants with pulmonary hemorrhage that were ≤30 weeks gestational age or premature infants with pulmonary hemorrhage who were hospitalized in our neonatal intensive care unit between 01 January 2013 and 31 December 2015 were evaluated. Group 1 (n = 21) received rFVIIa support within the first 30 min of pulmonary hemorrhage plus conventional treatment, while Group 2 (n = 21) received conventional treatment only. The number of patients whose pulmonary hemorrhage was stopped within the first 2 h was significantly higher in Group 1 than Group 2 (n = 14 vs n = 4; p = 0.002). After pulmonary hemorrhage, hemoglobin values of Group 1 were higher than Group 2 (11.12 ± 1.06 vs 10.14 ± 1.59 g/dL; p = 0.024). Erythrocyte suspension (1.43 ± 4.51 vs 5.71 ± 7.46 mL/kg; p = 0.030) and fresh frozen plasma use (5.71 ± 8.10 vs 19.52 ± 12.44 mL/kg; p premature infants.

  18. Recombinant factor VIIa is associated with an improved 24-hour survival without an improvement in inpatient survival in massively transfused civilian trauma patients

    Directory of Open Access Journals (Sweden)

    Bartolomeu Nascimento

    2011-01-01

    Full Text Available OBJECTIVE: To determine whether recombinant factor VIIa (rFVIIa is associated with increased survival and/or thromboembolic complications. INTRODUCTION: Uncontrollable hemorrhage is the main cause of early mortality in trauma. rFVIIa has been suggested for the management of refractory hemorrhage. However, there is conflicting evidence about the survival benefit of rFVIIa in trauma. Furthermore, recent reports have raised concerns about increased thromboembolic events with rFVIIa use. METHODS: Consecutive massively transfused (> 8 units of red blood cells within 12 h trauma patients were studied. Data on demographics, injury severity scores, baseline laboratory values and use of rFVIIa were collected. Rate of transfusion in the first 6 h was used as surrogate for bleeding. Study outcomes included 24-hour and in-hospital survival, and thromboembolic events. A multivariable logistic regression analysis was used to determine the impact of rFVIIa on 24-hour and in-hospital survival. RESULTS: Three-hundred and twenty-eight patients were massively transfused. Of these, 72 patients received rFVIIa. As expected, patients administered rFVIIa had a greater degree of shock than the non-rFVIIa group. Using logistic regression to adjust for predictors of death in the regression analysis, rFVIIa was a significant predictor of 24-hour survival (odds ratio (OR = 2.65; confidence interval 1.26-5.59; p = 0.01 but not of in-hospital survival (OR = 1.63; confidence interval 0.79-3.37; p = 0.19. No differences were seen in clinically relevant thromboembolic events. CONCLUSIONS: Despite being associated with improved 24-hour survival, rFVIIa is not associated with a late survival to discharge in massively transfused civilian trauma patients.

  19. Use of recombinant factor VIIa (rFVIIa as pre-hospital treatment in a swine model of fluid percussion traumatic brain injury

    Directory of Open Access Journals (Sweden)

    Bobby Kim

    2014-01-01

    Full Text Available Context: Recombinant factor VIIa (rFVIIa has been used as an adjunctive therapy for acute post-traumatic hemorrhage and reversal of iatrogenic coagulopathy in trauma patients in the hospital setting. However, investigations regarding its potential use in pre-hospital management of traumatic brain injury (TBI have not been conducted extensively. Aims: In the present study, we investigated the physiology, hematology and histology effects of a single pre-hospital bolus injection of rFVIIa compared to current clinical practice of no pre-hospital intervention in a swine model of moderate fluid percussion TBI. Materials and Methods: Animals were randomized to receive either a bolus of rFVIIa (90 μg/kg or nothing 15 minutes (T15 post-injury. Hospital arrival was simulated at T60, and animals were euthanized at experimental endpoint (T360. Results: Survival was 100% in both groups; baseline physiology parameters were similar, vital signs were comparable. Animals that received rFVIIa demonstrated less hemorrhage in subarachnoid space (P = 0.0037 and less neuronal degeneration in left hippocampus, pons, and cerebellum (P = 0.00009, P = 0.00008, and P = 0.251, respectively. Immunohistochemical staining of brain sections showed less overall loss of microtubule-associated protein 2 (MAP2 and less Flouro-Jade B positive cells in rFVIIa-treated animals. Conclusions: Early pre-hospital administration of rFVIIa in this swine TBI model reduced neuronal necrosis and intracranial hemorrhage (ICH. These results merit further investigation of this approach in pre-hospital trauma care.

  20. RECOMBINANT FACTOR VIIa – NEW TREATMENT OPTION FOR CONTROL OF INTRACTABLE BLEEDING IN SURGICAL AND TRAUMA PATIENTS AND IN OTHER HAEMOSTASIS DISORDERS

    Directory of Open Access Journals (Sweden)

    Samo Zver

    2004-12-01

    Full Text Available Background. Recombinant factor VIIa (rFVIIa, which is currently registered only for the treatment of haemophilia A and B patients with inhibitors, is seen increasingly as a possible universal haemostatic agent in untractable bleedings. One possible mechanism for the efficacy rFVIIa may be a consequence of it’s from the tissue factor (TF and from the level of disfunction in haemostatic system independant activity, which generates »thrombin burst« formation. It seems that rFVIIa remains active only at the site of tissue injury/bleeding.Conclusions. There are two components of bleeding in surgery and trauma patients. One is bleeding from large calibre arteries and veins which requires surgical intervention. The other, which goes along with the first one, is coagulopathic bleeding. The latter is a consequence of consumptional and dilutional coagulopathy, hypothermia, multitransfusion syndrom and metabolic disbalances in patients. rFVIIa effects coagulopathic component of the bleeding. For effective treatment with rFVIIa in such patients, replacement therapy with erythrocytes, platelets and fresh frozen plasma is mandatory and requires a haematologist assistance in the treatment strategy.Most reported cases of effective rFVIIa usage are from the field of traumatology. Until now, there have been no universal recommendations when to start treatment with rFVIIa in a bleeding trauma patient. Most experience with rFVIIa are from Israel and their recommendations are perhaps the most valuable ones. rFVIIa was used several times during intra-operative and post-operative bleeding episodes. There are reports of clinical studies and usage in patients with/ after prostate surgery, cardiovascular operations and liver transplants.There are data about effective rFVIIa usage in neurology and neurosurgery patients (intracranial haemorrhages, obstetrics and gynecology field. Possible future indications are thrombocytopenias, thrombocytopathias (Glanzmann

  1. De novo assembly and characterization of the transcriptome of seagrass Zostera marina using Illumina paired-end sequencing.

    Directory of Open Access Journals (Sweden)

    Fanna Kong

    Full Text Available BACKGROUND: The seagrass Zostera marina is a monocotyledonous angiosperm belonging to a polyphyletic group of plants that can live submerged in marine habitats. Zostera marina L. is one of the most common seagrasses and is considered a cornerstone of marine plant molecular ecology research and comparative studies. However, the mechanisms underlying its adaptation to the marine environment still remain poorly understood due to limited transcriptomic and genomic data. PRINCIPAL FINDINGS: Here we explored the transcriptome of Z. marina leaves under different environmental conditions using Illumina paired-end sequencing. Approximately 55 million sequencing reads were obtained, representing 58,457 transcripts that correspond to 24,216 unigenes. A total of 14,389 (59.41% unigenes were annotated by blast searches against the NCBI non-redundant protein database. 45.18% and 46.91% of the unigenes had significant similarity with proteins in the Swiss-Prot database and Pfam database, respectively. Among these, 13,897 unigenes were assigned to 57 Gene Ontology (GO terms and 4,745 unigenes were identified and mapped to 233 pathways via functional annotation against the Kyoto Encyclopedia of Genes and Genomes pathway database (KEGG. We compared the orthologous gene family of the Z. marina transcriptome to Oryza sativa and Pyropia yezoensis and 11,667 orthologous gene families are specific to Z. marina. Furthermore, we identified the photoreceptors sensing red/far-red light and blue light. Also, we identified a large number of genes that are involved in ion transporters and channels including Na+ efflux, K+ uptake, Cl- channels, and H+ pumping. CONCLUSIONS: Our study contains an extensive sequencing and gene-annotation analysis of Z. marina. This information represents a genetic resource for the discovery of genes related to light sensing and salt tolerance in this species. Our transcriptome can be further utilized in future studies on molecular adaptation to

  2. De novo assembly and characterization of the transcriptome of seagrass Zostera marina using Illumina paired-end sequencing.

    Science.gov (United States)

    Kong, Fanna; Li, Hong; Sun, Peipei; Zhou, Yang; Mao, Yunxiang

    2014-01-01

    The seagrass Zostera marina is a monocotyledonous angiosperm belonging to a polyphyletic group of plants that can live submerged in marine habitats. Zostera marina L. is one of the most common seagrasses and is considered a cornerstone of marine plant molecular ecology research and comparative studies. However, the mechanisms underlying its adaptation to the marine environment still remain poorly understood due to limited transcriptomic and genomic data. Here we explored the transcriptome of Z. marina leaves under different environmental conditions using Illumina paired-end sequencing. Approximately 55 million sequencing reads were obtained, representing 58,457 transcripts that correspond to 24,216 unigenes. A total of 14,389 (59.41%) unigenes were annotated by blast searches against the NCBI non-redundant protein database. 45.18% and 46.91% of the unigenes had significant similarity with proteins in the Swiss-Prot database and Pfam database, respectively. Among these, 13,897 unigenes were assigned to 57 Gene Ontology (GO) terms and 4,745 unigenes were identified and mapped to 233 pathways via functional annotation against the Kyoto Encyclopedia of Genes and Genomes pathway database (KEGG). We compared the orthologous gene family of the Z. marina transcriptome to Oryza sativa and Pyropia yezoensis and 11,667 orthologous gene families are specific to Z. marina. Furthermore, we identified the photoreceptors sensing red/far-red light and blue light. Also, we identified a large number of genes that are involved in ion transporters and channels including Na+ efflux, K+ uptake, Cl- channels, and H+ pumping. Our study contains an extensive sequencing and gene-annotation analysis of Z. marina. This information represents a genetic resource for the discovery of genes related to light sensing and salt tolerance in this species. Our transcriptome can be further utilized in future studies on molecular adaptation to abiotic stress in Z. marina.

  3. Late Miocene diversification and phylogenetic relationships of the huge toads in the Rhinella marina (Linnaeus, 1758) species group (Anura: Bufonidae).

    Science.gov (United States)

    Maciel, Natan Medeiros; Collevatti, Rosane Garcia; Colli, Guarino Rinaldi; Schwartz, Elisabeth Ferroni

    2010-11-01

    We investigated the phylogeny and biogeography of the Rhinella marina group, using molecular, morphological, and skin-secretion data, contributing to an understanding of Neotropical faunal diversification. The maximum-parsimony and Bayesian analyzes of the combined data recovered a monophyletic R. marina group. Molecular dating based on Bayesian inferences and fossil calibration placed the earliest phylogenetic split within the R. marina group at ∼ 10.47 MYA, in the late Miocene. Two rapid major diversifications occurred from Central Brazil, first northward (∼ 8.08 MYA) in late Miocene and later southward (∼ 5.17 MYA) in early Pliocene. These results suggest that barriers and dispersal routes created by the uplift of Brazilian Central Shield and climatic changes explain the diversification and current species distributions of the R. marina group. Dispersal-vicariance analyzes (DIVA) indicated that the two major diversifications of the R. marina group were due to vicariance, although eleven dispersals subsequently occurred. Copyright © 2010 Elsevier Inc. All rights reserved.

  4. ELIMINASI LOGAM Cu OLEH SERASAH Avicennia marina DI LINGKUNGAN TAMBAK BANDENG WILAYAH TAPAK TUGUREJO, SEMARANG (Cu Metal Elimination by Avicennia marina Litter Leaf in the Environment of Milkfish Fishpond in Tapak Village, Tugurejo Semarang

    Directory of Open Access Journals (Sweden)

    Nana Kariada Tri Martuti

    2016-09-01

    Full Text Available ABSTRAK Serasah mempunyai peran penting dalam transfer logam dari tanaman ke dalam media lingkungan hidupnya. Tujuan penelitian mengkaji tingkat eliminasi logam Cu melalui proses defoliasi serasah daun Avicennia marina di tambak bandeng. Bahan penelitian terdiri dari air dan sedimen tambak serta serasah daun mangrove dari jenis A. marina. Destruksi logam Cu pada air, sedimen dan serasah menggunakan Tefflon bomb extraction, dilanjutkan dengan pengukuran logam Cu menggunakan AAS. Penelitian dilakukan selama 90 hari pada tambak bandeng wilayah Tapak Semarang, di dalamnya terdapat tumbuhan mangrove A. marina. Kadar logam Cu pada air tambak penelitian antara 0,02±0,007 - 0,05±0,008 mg/L. Konsentrasi logam Cu pada sedimen tambak penelitian berkisar antara 15,20±1,77 - 25,03 ±4,77 mg/kg, Faktor Konsentrasi (FK air dan sedimen tambak penelitian antara 500.52 - 897,70. Serasah daun A. marina mengandung logam Cu 2,346±0,536 – 5,285±2,091 mg/kg. Hasil penelitian menunjukkan terdapat kemampuan serasah A. marina dalam mengembalikan logam Cu ke lingkungan hidupnya. Perbandingan antara serasah dengan air dan sedimen menunjukkan bahwa kemampuan serasah dalam mengembalikan Cu ke perairan (46,92 - 228,72 lebih besar dari pada ke sedimen lingkungan hidupnya (0,094 - 0,318. ABSTRACT Avicennia marina litter plays an important role in the heavy metal transfer from the plant into its environment. It occurs due to its ability in eliminating the metal from its tissue, as its adaptation to metal – polluted environment. The purpose of this research was to examine the elimination level of Cu through a defoliation process of A. marina into milkfish pond. The objects of the research were water, sediment and A. marina litter. The destruction of Cu in water, sediment and litter was accomplished using Tefflon bomb extraction, followed by measurement of Cu using AAS. The study was conducted in milkfish pond in Tapak region at Semarang city for 90 days, in which the A

  5. A tale of two seagrasses: Comparing the science and management of Zostera marina and Zostera japonica in the Pacific Northwest - CERF

    Science.gov (United States)

    On the Pacific coast of North America, at least two congeners of Zostera occur: native Z. marina, and introduced, Z. japonica. Z. marina is protected by State and Federal laws as essential fish habitat. Z. japonica is considered “invasive” and therefore, ecologicall...

  6. Ekstrak daun Avicennia marina sebagai anti jamur pada telur ikan mas, Cyprinus carpio (Avicennia marina leaf extracts as an anti fungal for common carp, Cyprinus carpio eggs

    Directory of Open Access Journals (Sweden)

    Sofyatuddin Karina

    2016-12-01

    Full Text Available The objective of this research was to determine the effect and the optimum concentration of Avicennia leaf extracts on prevalence, hatching rate and hatching time of common carp eggs (Cyprinus carpio that infected by Saprolegnia sp. The research was conducted at Balai Benih Ikan (BBI Lukup Badak, Aceh Tengah District in June 2016. The completely randomized design was used as statistical analysis method in this study with five treatments and 4 replications. The fertilized eggs of common carp were infected by Saporolegnia sp. then treated by immersing in the extract. The treatments were the different concentrations of extract, namely; 0, 5, 10, 15 and 20 ppm. The result of ANOVA test showed that the extract Avecenia leaf extracts gave the significant effect on the prevalence, hatching rate and hatching time of common carp eggs (P<0.05. The best results for all parameters were recorded at the concentration of 10 ppm. Therefore, it is concluded that the optimum concentration is 10 ppm. Penelitian ini bertujuan untuk mengetahui pengaruh dan konsentrasi optimum ekstrak daun Avicennia marina terhadap prevalensi, daya tetas serta waktu tetas telur ikan mas (Cyprinus carpio yang terinfeksi Saprolegnia sp. Penelitian dilakukan di Balai Benih Ikan (BBI Lukup Badak, Kab. Aceh Tengah pada bulan Juni 2016. Analisis statistik menggunakan metode Rancangan Acak Lengkap (RAL dengan 5 taraf perlakuan dan 4 kali ulangan. Sampel yang digunakan adalah telur ikan mas yang telah terbuahi dan terinfeksi dengan jamur Saprolegnia sp. kemudian direndam dengan ekstrak. Perlakuan yang dilakukan meliputi perlakuan konsentrasi ekstrak yaitu: 0, 5, 10, 15, 20 ppm. Hasil uji ANOVA menunjukkan bahwa ekstrak daun Avicennia marina berpengaruh nyata (P<0,05 terhadap prevalensi serangan jamur, daya tetas serta waktu tetas telur ikan mas. Nilai terbaik untuk semua parameter yang diukur ditemukan pada konsentrasi 10 ppm ekstrak. Oleh karena itu disimpulkan bahwa konsentrasi yang

  7. The Existing Condition of Mangrove Region of Avicenia marina, Its: Distribution and Functional Transformation

    Directory of Open Access Journals (Sweden)

    Ahmad Herison

    2014-04-01

    Full Text Available Mangrove ecosystem existence is important for environment and other organisms because of its ecological and economical values, so that management and preservation of mangrove ecosystem are needed. The purpose of this research was to determine the existing condition of mangrove, both its distribution and its functional transformation in Indah Kapuk Coastal Area. Avicennia marina  becomes important as wave attenuation,  a form of abrasion antidote. Transect-Square and Spot-Check methods were used to determine the existing condition of A.marina mangrove forests. Autocad program, coordinate converter, Google Earth, Google Map, and Arc View were applied in process of making mangrove distribution map. In western of research location exactly at Station 1 and Station 2, the density value of mangrove was 450 and 825 tree ha-1, respectively with  sparse category because they were contaminated by waste and litter. In eastern of research location namely Station 3, Station 4, and Station 5 the mangroves grow well with density value of 650 (sparse, 1,500 (very dense, and 1,200 tree ha-1 (fair, respectively, eventhough the contamination still happened. The mangrove forests around the stations do not function as wave attenuation because there were many waterfront constructions which have replaced the function of mangrove forests to damp the wave. In short, it can be stated that the mangrove's function has changed in a case of wave attenuation. The function of mangrove forests is not determined by mangrove forest density but it is determined by mangrove's free position.Keywords: Avicennia marina, mangrove, wave attenuation, waterfront constructions

  8. Antiproliferative activity and chemical composition of the venom from the Amazonian toad Rhinella marina (Anura: Bufonidae).

    Science.gov (United States)

    Schmeda-Hirschmann, Guillermo; Quispe, Cristina; Arana, Gabriel Vargas; Theoduloz, Cristina; Urra, Félix A; Cárdenas, César

    2016-10-01

    Little is known on the composition of Peruvian Amazon toad venoms. The large toad Rhinella marina is common in the cleared tropical forests of the Iquitos region and is regarded as poisonous. The venom from two different populations of R. marina was collected in the Departamento de Loreto, Perú. The samples were assessed for antiproliferative effect and composition. Some 29 compounds were identified or tentatively identified from the venom by spectroscopic and spectrometric means. The main free bufadienolide was marinobufagin 7 while marinobufotoxin 15 and bufalitoxin 9 were the main bufadienolide argininyl diacid derivatives. The alkaloids dehydrobufotenin 28 and bufotenidin 29 were present in both venoms. The main difference in the venoms was the relative ratio of argininyl diacids from bufadienolides to free bufadienolides. The argininyl diacids included derivatives from bufalin, marinobufagin, telocinobufagin, hellebrigenin, resibufogenin and bufotalinin. Four compounds, including undecadienoyl aginine 6 and three argininyl diacids from bufadienolides were tentatively identified for the first time in the samples. The venom showed a strong antiproliferative effect towards MRC-5 normal human lung fibroblasts (0.063-0.247 μg/mL), AGS human gastric adenocarcinoma cells (0.076-0.272 μg/mL), SK-MES-1 human lung cancer cells (0.154-0.296 μg/mL), J82 human bladder carcinoma cells (0.169-0.212 μg/mL), and HL-60 human promyelocytic leukemia (0.071-0.283 μg/mL). The antiproliferative effect is mediated by ROS production and cell cycle arrest in human breast cancer cells (MCF7 and MDA-MB-231). This is the first report on the composition of R. marina venom from the Peruvian Amazon pointing out the need to include different venom samples to get a better picture from the activity and composition of South American toad defense substances. Copyright © 2016 Elsevier Ltd. All rights reserved.

  9. Photosystem Trap Energies and Spectrally-Dependent Energy-Storage Efficiencies in the Chl d-Utilizing Cyanobacterium, Acaryochloris Marina

    Science.gov (United States)

    Mielke, Steven P.; Kiang, Nancy Y.; Blankenship, Robert E.; Mauzerall, David

    2012-01-01

    Acaryochloris marina is the only species known to utilize chlorophyll (Chl) d as a principal photopigment. The peak absorption wavelength of Chl d is redshifted approx. 40 nm in vivo relative to Chl a, enabling this cyanobacterium to perform oxygenic phototrophy in niche environments enhanced in far-red light. We present measurements of the in vivo energy-storage (E-S) efficiency of photosynthesis in A. marina, obtained using pulsed photoacoustics (PA) over a 90-nm range of excitation wavelengths in the red and far-red. Together with modeling results, these measurements provide the first direct observation of the trap energies of PSI and PSII, and also the photosystem-specific contributions to the total E-S efficiency. We find the maximum observed efficiency in A. marina (40+/-1% at 735 nm) is higher than in the Chl a cyanobacterium Synechococcus leopoliensis (35+/-1% at 690 nm). The efficiency at peak absorption wavelength is also higher in A. marina (36+/-1% at 710 nm vs. 31+/-1% at 670 nm). In both species, the trap efficiencies are approx. 40% (PSI) and approx. 30% (PSII). The PSI trap in A. marina is found to lie at 740+/-5 nm, in agreement with the value inferred from spectroscopic methods. The best fit of the model to the PA data identifies the PSII trap at 723+/-3 nm, supporting the view that the primary electron-donor is Chl d, probably at the accessory (ChlD1) site. A decrease in efficiency beyond the trap wavelength, consistent with uphill energy transfer, is clearly observed and fit by the model. These results demonstrate that the E-S efficiency in A. marina is not thermodynamically limited, suggesting that oxygenic photosynthesis is viable in even redder light environments.

  10. An assessment of site suitability for marina construction in Istanbul, Turkey, using GIS and AHP multicriteria decision analysis.

    Science.gov (United States)

    Gumusay, Mustafa Umit; Koseoglu, Gokalp; Bakirman, Tolga

    2016-12-01

    Marinas play a key role in sea transportation and tourism. The problem of an insufficient marina capacity has revealed in terms of sea traffic due to the demographic structure and increasing tourism potential of Istanbul which is the biggest metropolitan city of Turkey and has around 600-km-long coastline. Therefore, the study area is mainly focused on the Marmara Sea shoreline of Istanbul. Rather than traditional methods, a rapid and cost-effective solution which considers natural and urban environment conditions is essential to satisfy the need for a marina site selection. Thanks to the latest improvements in geographic information systems, it is convenient to perform location selection analysis of marinas taking advantages of geology, land use, demography and accessibility data sets. The goal of this study is to define the areas that are appropriate for building marinas, with the use of topographic and demographic data in a present shoreline applying analytical hierarchy process multicriteria decision-making method. In this study, erosion, landslide, tsunami, land use, geologically hazardous areas, transfer lines, sea traffic data, neighbourhood scale population, age patterns and house income data have been used. Analytical hierarchy process method is used to give a weight to each data set, and a grading system has been developed for the area selection of marinas. The result maps of the analysis that show study area as classified into four categories from good to not suitable are presented. It is possible to create a decision support system for upper scale plans that enable authorities to perform analysis accurately, cost and time effectively using the proposed methodology that integrates multiple data sets with different scales and types.

  11. Factor XIII and tranexamic acid but not recombinant factor VIIa attenuate tissue plasminogen activator-induced hyperfibrinolysis in human whole blood.

    Science.gov (United States)

    Dirkmann, Daniel; Görlinger, Klaus; Gisbertz, Caroline; Dusse, Fabian; Peters, Jürgen

    2012-06-01

    Hyperfibrinolysis is a pathological state that often results in depletion of coagulation factors and platelets and can contribute to bleeding. Factor XIII (FXIII) and thrombin activatable fibrinolysis inhibitor have key roles in protecting clots against fibrinolysis. We tested the hypotheses that FXIII concentrate, prothrombin complex concentrate (PCC), recombinant factor VIIa (rFVIIa), and tranexamic acid (TA) inhibit fibrinolysis to different degrees, and that platelets contribute to antifibrinolysis. Hyperfibrinolysis was induced by addition of recombinant tissue plasminogen activator (r-tPA) (final concentration: 100 ng · mL(-1)) to citrated whole blood obtained from 13 healthy volunteers. To assess inhibition of fibrinolysis, we added to the assays FXIII-A(2)B(2) (0.42 U · mL(-1)), PCC (0.42 U · mL(-1)), rFVIIa (final concentration: 1.6 μg · mL(-1)), TA (final concentration: 0.33 mg · mL(-1)), or saline. Coagulation was analyzed by rotational thromboelastometry (ROTEM®) using the clot lysis index (CLI) after 45 and 60 minutes in extrinsically activated assays, with (FIBTEM®) and without (EXTEM®) inhibition of platelet function by cytochalasin D. After r-tPA-evoked fibrinolysis (CLI45: median 78%; 72/85.5, 25th/75th percentile), FXIII (90%; 82.5/96, P = 0.025), PCC (89%; 74/91, P = 0.0465), and TA (94%; 92/96, P = 0.001) but not rFVIIa (79%; 72/86.5, P = 1.0) significantly attenuated the decrease in CLI. Similarly, CLI60 increased only with FXIII (66%; 33/90.5, P = 0.017) and TA (90%; 89/92, P = 0.001) compared with r-tPA alone (21%; 7/59). After abolition of platelet function by cytochalasin D, only TA (95%; 89/97.5, P = 0.0025) and PCC (84%; 70.5/90, P = 0.0305) but not FXIII or rFVIIa significantly increased CLI45 and CLI60 (TA: 89%; 84.5/96, P = 0.01 and PCC: 55%; 29.5/60, P = 0.0405) compared with r-tPA alone (CLI45: 59%; 40.5/72.5 and CLI60: 10%; 0/30). In thromboelastometric assays using whole blood, only TA, FXIII, and PCC significantly

  12. Foleyellides rhinellae sp. nov. (Nematoda, Onchocercidae) a new filaria parasitizing Rhinella marina (Anura, Bufonidae) in Mexico.

    Science.gov (United States)

    García-Prieto, Luis; Ruiz-Torres, Nallely; Osorio-Sarabia, David; Merlo-Serna, Aldo

    2014-09-01

    A new nematode species, Foleyellides rhinellae sp. nov. (Onchocercidae), is described from specimens found in the body cavity of the cane toad, Rhinella marina (Linnaeus) (Anura, Bufonidae), in the Laguna de Coyuca, Guerrero, in the Pacific slope of Mexico. The new species differs from the other nine species of Foleyellides by infecting bufonid anurans and by the number and arrangement of caudal papillae. Other distinguishing feature of the new species is the size of the left spicule (0.16-0.23 long), the smallest recorded among the species included in the genus. Foleyellides rhinellae sp. nov. is the second known species of the genus recorded from amphibians of Mexico.

  13. New Antioxidative Secondary Metabolites from the Fruits of a Beibu Gulf Mangrove, Avicennia marina

    Directory of Open Access Journals (Sweden)

    Cheng-Hai Gao

    2014-07-01

    Full Text Available Further chemical investigation of the fruits of the mangrove, Avicennia marina, afforded three new phenylethyl glycosides, marinoids J–L (1–3, and a new cinnamoyl glycoside, marinoid M (4. The structures of isolates were elucidated on the basis of extensive spectroscopic analysis and by comparison of the data with those of related secondary metabolites. The antioxidant activity of the isolates was evaluated using the cellular antioxidant assay (CAA, and compounds 1–4 showed antioxidant activities, with EC50 values ranging from 23.0 ± 0.71 μM to 247.8 ± 2.47 μM.

  14. Dispositivo para aprovechar la energía de corrientes marinas

    OpenAIRE

    López Piñeiro, Amable; Somolinos Sánchez, José Andrés; Núñez Rivas, Luis Ramón; Novoa Rojas, Eva María; Carneros Lozano, Alfonso M.

    2012-01-01

    En este articulo se expone el proceso seguido en la definición y desarrollo de un generador, denominado GESMEY, para el aprovechamiento de la energía de las corrientes marinas situadas a más de 40 metros de profundidad, lo que junto a sus características definitorias que le permiten evolucionar en el seno marino sólo utilizando fuerzas hidrodinámicas, le definen como un dispositivo con mejores prestaciones que los existentes a la fecha. Por ello puede afirmarse que GESMEY es ya un dispositivo...

  15. Obtención de energía a partir de las corrientes marinas

    OpenAIRE

    Echeverría Cabodevilla, Álvaro

    2017-01-01

    El objetivo del proyecto es evaluar la viabilidad de una granja de energía hidrocinética en los territorios marinos españoles. Como se puede observar en el desarrollo del estudio, el resultado es negativo. En España no existe una gran zona que pueda ser utilizada para crear una granja de energía hidrocinética. La energía hidrocinética es aquella que utiliza la energía cinética del agua marina para obtener electricidad, principalmente los movimientos de agua de las corrientes ma...

  16. El potencial energético útil, de las corrientes marinas en el estrecho de Gibraltar

    OpenAIRE

    Juanes González, Juan Manuel

    2011-01-01

    La necesidad de encontrar fuentes de energías renovables ha hecho que en estos últimos años se hallan desarrollado una serie de dispositivos para aprovechar las mareas y las corrientes marinas. Esta tesis trata de establecer la capacidad energética de las corrientes y mareas del estrecho de Gibraltar en una amplia zona de estudio y los emplazamientos idóneos para la instalación de dispositivos de aprovechamiento de las corrientes y mareas en la zona. Para ello se propone un método de e...

  17. Mecanismos de toxicidad y permeabilidad transepitelial de toxinas marinas y de agua dulce

    OpenAIRE

    Fernández Martínez, Diego Alberto

    2015-01-01

    La presente tesis doctoral aporta datos experimentales que permiten comprender mejor los efectos que las toxinas marinas Palitoxina (PLTX), Ácido Okadaico (OA), Dinofisistoxina 1 y 2 (DTX-1 y DTX-2) y la toxina de agua dulce Cilindrospermopsina (CYN), pueden ejercer sobre la salud humana y animal. Con esta finalidad se investiga la citotoxicidad y las alteraciones citomorfológicas que estos compuestos desencadenan en células intestinales, hepáticas y nerviosas. También se evalú...

  18. Biodisponibilidad y especiación de arsénico en las algas marinas

    OpenAIRE

    García Sartal, Cristina

    2013-01-01

    Las algas, desde un punto de vista alimenticio, constituyen una fuente de proteínas, aminoácidos esenciales, vitaminas, lípidos y minerales. Sin embargo, las algas también pueden acumular elementos no esenciales procedentes del agua marina circundante, entre ellos, el arsénico, elemento ampliamente reconocido por su toxicidad. La toxicidad del arsénico depende fundamentalmente de su especiación, resultando más toxicas las especies inorgánicas de arsénico que sus correspondie...

  19. Estudio de la influencia de factores ecológicos sobre comunidades marinas de algas

    OpenAIRE

    López Alcarria, Carolina

    2015-01-01

    Las algas utilizan distintas estrategias para adaptarse a los factores que condicionan el medio que las rodea. Dependiendo de las estrategias utilizadas se pueden clasificar en diferentes grupos funcionales. Los grupos funcionales descritos por Grime (1977) para las plantas sirven para clasificar las algas y para evaluar la calidad ecológica y de las aguas que bañan a estas comunidades. Se han estudiado distintas comunidades de algas marinas en Tabarca, Cabo de las Huertas y en Agua Amarga pa...

  20. Biosorption OF Cu (II) by pretreated marina algae Grateloupia doryophora (Rhodophyta)

    OpenAIRE

    Rivera P., Jilver; Departamento de Fisicoquímica, Facultad de Química e lng. Química, Universidad Nacional Mayor de San Marcos. Lima, Perú.; Tapia H., Nelson; Departamento de Fisicoquímica, Facultad de Química e lng. Química, Universidad Nacional Mayor de San Marcos. Lima, Perú; Córdova C., César; Facultad de Biología. Universidad Nacional Mayor de San Marcos. Lima, Perú; Yarango R., Alejandro; Departamento de Fisicoquímica, Facultad de Química e lng. Química, Universidad Nacional Mayor de San Marcos. Lima, Perú; Torres D., Francisco; Departamento de Fisicoquímica, Facultad de Química e lng. Química, Universidad Nacional Mayor de San Marcos. Lima, Perú; Rojas P., Nora; Departamento de Fisicoquímica, Facultad de Química e lng. Química, Universidad Nacional Mayor de San Marcos. Lima, Perú; Caja R., Víctor; Departamento de Fisicoquímica, Facultad de Química e lng. Química, Universidad Nacional Mayor de San Marcos. Lima, Perú; Galarreta D., Hugo; Departamento de Fisicoquímica, Facultad de Química e lng. Química, Universidad Nacional Mayor de San Marcos. Lima, Perú

    2014-01-01

    Copper (11) adsorption properties of pre-treated biomass of marina algae Grateloupia doryophora (Rhodophyta) were investigated. The native biomass was treated with 0.2 M CaCl2, solution for 24 h under slow stirring. The solution pH was kept constant at pH = 5.0 using 0.1 M NaOH. The treated biomass was dried in an oven at 40ºC for 24 h. The adsorption capacity of the biomass strongly depends on equilibrium solution pH. At solution pH between 4 and 4,5 were observed the maximum adsorptive capa...

  1. Cadmium distribution in sediment and the lugworm Arenicola marina in a low concentration exposure experiment

    Digital Repository Service at National Institute of Oceanography (India)

    Everaarts, J.M.; Devi, K.S.

    Bull. Environ. Contam. Toxicol. (1996) 57:771-778 © 1996 Springer-Verlag New York Inc. Cadmium Distribution in Sediment and the Lugworm Arenicola marina in a Low Concentration Exposure Experiment J. M. Everaarts,1 K. SaralaDevi2 1Netherlands Institute... of adhering sediment and other particles before being used in the experiment. The experiment was carried out applying a continuous flow-through filtered seawater system with tidal movements, in a 15 °C (± 0.5 °C) temperature controlled room, with 12:12 hr...

  2. Antifouling biocides in German marinas: Exposure assessment and calculation of national consumption and emission.

    Science.gov (United States)

    Daehne, Dagmar; Fürle, Constanze; Thomsen, Anja; Watermann, Burkard; Feibicke, Michael

    2017-09-01

    The authorization of biocidal antifouling products for leisure boats is the subject of the European Union Biocides Regulation 528/2012. National specifics may be regarded by the member states in their assessment of environmental risks. The aim of this survey was to collect corresponding data and to create a database for the environmental risk assessment of antifouling active substances in German surface waters. Water concentrations of current antifouling active substances and selected breakdown products were measured in a single-sampling campaign covering 50 marinas at inland and coastal areas. Increased levels were found for Zn, Cu, and cybutryne. For the latter, the maximum allowable concentration according to Directive 2013/39/EU was exceeded at 5 marinas. For Cu, local environmental quality standards were exceeded at 10 marinas. Base data on the total boat inventory in Germany were lacking until now. For that reason, a nationwide survey of mooring berths was conducted by use of aerial photos. About 206 000 mooring berths obviously used by boats with a potential antifouling application were counted. The blind spot of very small marinas was estimated at 20 000 berths. Seventy-one percent of berths were located at freshwater sites, illustrating the importance of navigable inland waterways for leisure boat activities and underlining the need for a customized exposure assessment in these areas. Moreover, the national consumption of all antifouling products for leisure boats was calculated. The total amount of 794 tonnes/annum (t/a) consisted of 179 t/a of inorganic Cu compounds, 19 t/a of organic cobiocides, and 49.5 t/a of Zn. With regard to weight proportion, 141 t/a Cu and 40 t/a Zn were consumed. Assuming an emission ratio of 50% during service life, 70.5 t/a of Cu amounted to 15% of all external sources for Cu release to German surface waters. These figures highlight the need for mitigation measures. Integr Environ Assess Manag 2017;13:892-905. © 2017 The

  3. Calidad de las aguas en el período 2003-2009 en marina Gaviota Varadero

    OpenAIRE

    López García, D.; Blanco Concepción, M.

    2010-01-01

    En el presente trabajo se exponen los resultados de diez etapas de monitoreo ambiental, según lo dispuesto en el EsIA del Dragado a Marina Gaviota Varadero. El mismo tuvo como objetivo evaluar la calidad del agua y de los sedimentos, sobre la base de las mediciones de una serie de parámetros hidroquímicos y de contaminación además de la caracterización de la biota terrestre y marina, que permitieran valorar el estado del medio una vez concluidas las acciones ambientales, en la reg...

  4. Auditoría y evaluación energética de Marina Port Vell

    OpenAIRE

    Díaz Llobet, Mari Carmen; Suárez Gallegos, Dennys

    2014-01-01

    [CASTELLÀ] Marina Port Vell quiere ser una organización activa en la protección climática y reducción de emisiones de efecto invernadero. Por éste motivo, uno de sus objetivos el presente año es establecer la norma ISO: 50001 de gestión de energía. Para conseguirlo, la empresa Equipo de Ingenieros de Edificación SCP, desde marzo del presente año, ha procedido a realizar parte imprescindible de ese objetivo, que es la evaluación energética de los edificios de la marina, colabora...

  5. Local replacement of ammophila arenaria by medicago marina after foredune trampling disturbance

    Directory of Open Access Journals (Sweden)

    Ana Ramos Pereira

    2013-06-01

    Full Text Available Human trampling is amongst the major causes of foredune disturbance. it creates a diffuse path network and the opening of bare‑ground clearings within dense vegetation patches as a result of plant removal, namely of Ammophila arenaria. in undisturbed plant communities A. arenaria becomes dominant, and strongly inhibits the co‑existence of other species, due to its phalanx growth strategy and by decreasing sand mobility near the surface. However, the dominance of this dune‑builder may be locally reversed after trampling disturbance. Data gathered in three foredune ‑sites at Vila nova de Gaia during 2006, three years after a major intervention by the Municipality to manage beach access through the dunes, suggest that a higher abundance of Medicago marina is linked to an increased availability of recruitment sites, due to death of A. arenaria individuals. acting as a powerful sand stabiliser, M. marina creates favourable conditions for annual species colonization of paths and gaps, significantly changing sand movement and deposition along the foredune gradient.

  6. The genome of the seagrass Zostera marina reveals angiosperm adaptation to the sea

    KAUST Repository

    Olsen, Jeanine L.

    2016-01-27

    Seagrasses colonized the sea1 on at least three independent occasions to form the basis of one of the most productive and widespread coastal ecosystems on the planet2. Here we report the genome of Zostera marina (L.), the first, to our knowledge, marine angiosperm to be fully sequenced. This reveals unique insights into the genomic losses and gains involved in achieving the structural and physiological adaptations required for its marine lifestyle, arguably the most severe habitat shift ever accomplished by flowering plants. Key angiosperm innovations that were lost include the entire repertoire of stomatal genes3, genes involved in the synthesis of terpenoids and ethylene signalling, and genes for ultraviolet protection and phytochromes for far-red sensing. Seagrasses have also regained functions enabling them to adjust to full salinity. Their cell walls contain all of the polysaccharides typical of land plants, but also contain polyanionic, low-methylated pectins and sulfated galactans, a feature shared with the cell walls of all macroalgae4 and that is important for ion homoeostasis, nutrient uptake and O2/CO2 exchange through leaf epidermal cells. The Z. marina genome resource will markedly advance a wide range of functional ecological studies from adaptation of marine ecosystems under climate warming5, 6, to unravelling the mechanisms of osmoregulation under high salinities that may further inform our understanding of the evolution of salt tolerance in crop plants7.

  7. Rhinella marina (Amphibia: Bufonidae) Versus Rhabdias paraensis (Nematoda: Rhabdiasidae): Expanding the View on a Natural Infection.

    Science.gov (United States)

    Santos, Jeannie Nascimento Dos; da Silva, Djane Clarys Baia; Feitosa, Lucas Aristóteles das Neves; Furtado, Adriano Penha; Giese, Elane Guerreiro; de Vasconcelos Melo, Francisco Tiago

    2016-06-01

    Amphibian and reptile lungs are frequently infected with Rhabdias parasites, and this condition ultimately leads to reduced survival, performance, and growth because of granulomatous inflammation, nodule formation, and nematodal pneumonia onset. Here we investigate the histopathological features of naturally infected Rhinella marina by the lung nematode Rhabdias paraensis. A total of 10 host animals were captured in peridomiciliar areas in the eastern Brazilian Amazon, and anatomic-histological analyses were performed on both the infected and non-infected lungs of these amphibians. Helminths were usually found within the secondary and primary septa of infected lungs whereas parasites were not detected within vessels or adhering to tissues. In addition, we observed discrete erythrocytes, diapedesis foci, few granulocytes and erythrocytes in the interseptal spaces, discrete cell infiltration, and a small number of melanomacrophages, and no granulomas or cysts were observed. New aspects related to changes in tissue and helminth-host interactions are discussed for the relationship of R. paraensis × Rhi. marina from the Amazon region.

  8. Microplastics are taken up by mussels (Mytilus edulis) and lugworms (Arenicola marina) living in natural habitats.

    Science.gov (United States)

    Van Cauwenberghe, Lisbeth; Claessens, Michiel; Vandegehuchte, Michiel B; Janssen, Colin R

    2015-04-01

    We studied the uptake of microplastics under field conditions. At six locations along the French-Belgian-Dutch coastline we collected two species of marine invertebrates representing different feeding strategies: the blue mussel Mytilus edulis (filter feeder) and the lugworm Arenicola marina (deposit feeder). Additional laboratory experiments were performed to assess possible (adverse) effects of ingestion and translocation of microplastics on the energy metabolism (cellular energy allocation) of these species. Microplastics were present in all organisms collected in the field: on average 0.2 ± 0.3 microplastics g(-1) (M. edulis) and 1.2 ± 2.8 particles g(-1) (A. marina). In a proof of principle laboratory experiment, mussels and lugworms exposed to high concentrations of polystyrene microspheres (110 particles mL(-1) seawater and 110 particles g(-1) sediment, respectively) showed no significant adverse effect on the organisms' overall energy budget. The results are discussed in the context of possible risks as a result of the possible transfer of adsorbed contaminants. Copyright © 2015 Elsevier Ltd. All rights reserved.

  9. EVALUACIÓN DE ACTITUDES FRENTE AL USO DE LAS TORTUGAS MARINAS EN PLAYA GANDOCA

    Directory of Open Access Journals (Sweden)

    M Malaver

    2008-01-01

    Full Text Available en el refugio nacional de vida silvestre Gandoca-Manzanillo, Costa rica, se creó en 1986 un programa para la protección y conservación de la población anidadora de tortugas marinas. Esta evaluación buscaba identificar los aspectos que predisponen a la población a actuar de una forma determinada utilizando el modelo metodológico denominado teoría de facetas; este modelo parte de una frase mapa que enmarca la hipótesis, y con la cual se elaboró el cuestionario de evaluación que se aplicó a 106 habitantes de la comunidad de Gandoca, lo que corresponde al 40% del total de pobladores según el instituto nacional de estadística y Censos (2000. en general, los resultados mostraron que la actitud de la población de Gandoca hacia el uso que se le debe dar a las tortugas marinas se basa en el elemento emocional, donde el turismo tiene gran aceptación, y donde existe en limitada medida la responsabilidad de las acciones hacia el objeto de la actitud.

  10. Transcriptomic resilience to global warming in the seagrass Zostera marina, a marine foundation species

    Science.gov (United States)

    Franssen, Susanne U.; Gu, Jenny; Bergmann, Nina; Winters, Gidon; Klostermeier, Ulrich C.; Rosenstiel, Philip; Bornberg-Bauer, Erich; Reusch, Thorsten B. H.

    2011-01-01

    Large-scale transcription profiling via direct cDNA sequencing provides important insights as to how foundation species cope with increasing climatic extremes predicted under global warming. Species distributed along a thermal cline, such as the ecologically important seagrass Zostera marina, provide an opportunity to assess temperature effects on gene expression as a function of their long-term adaptation to heat stress. We exposed a southern and northern European population of Zostera marina from contrasting thermal environments to a realistic heat wave in a common-stress garden. In a fully crossed experiment, eight cDNA libraries, each comprising ∼125 000 reads, were obtained during and after a simulated heat wave, along with nonstressed control treatments. Although gene-expression patterns during stress were similar in both populations and were dominated by classical heat-shock proteins, transcription profiles diverged after the heat wave. Gene-expression patterns in southern genotypes returned to control values immediately, but genotypes from the northern site failed to recover and revealed the induction of genes involved in protein degradation, indicating failed metabolic compensation to high sea-surface temperature. We conclude that the return of gene-expression patterns during recovery provides critical information on thermal adaptation in aquatic habitats under climatic stress. As a unifying concept for ecological genomics, we propose transcriptomic resilience, analogous to ecological resilience, as an important measure to predict the tolerance of individuals and hence the fate of local populations in the face of global warming. PMID:22084086

  11. EVALUACIÓN CITÓTOXICA DE FRACCIONES DE ESPONJAS MARINAS DEL CARIBE COLOMBIANO

    Directory of Open Access Journals (Sweden)

    NATALIA ESTRADA-ORTIZ

    2013-01-01

    Full Text Available En la búsqueda de actividad antitumoral en el desarrollo de fármacos, se requiere evaluar la actividad citotóxica de manera preliminar, con el fin de realizar otras potenciales actividades como antitproliferación y efecto genotóxico. En la evaluación de diferentes fracciones obtenidas de esponjas marinas del Caribe Colombiano se ha encontrado actividad antiproliferativa en algunas de ellas. Por lo anterior, en este trabajo se evaluó la actividad citotóxica de trece fracciones obtenidas de las esponjas marinas Amphimedon compressa, Cinachyrella kuekenthali, Svenzea zeai e Ircinia campana para determinar su potencial citotóxico, en las líneas celulares Jurkat clon E6-1 y CHO-K1. Se emplearon las técnicas de MTT y coloración vital de Azul de Tripano para evaluar su citotoxicidad y viabilidad para determinar su concentración inhibitoria media. En conclusión, los resultados muestran que ninguna fracción, presenta actividad citotóxica significativa.

  12. Genome sequence of the ocean sediment bacterium Saccharomonospora marina type strain (XMU15T)

    Energy Technology Data Exchange (ETDEWEB)

    Klenk, Hans-Peter [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Lu, Megan [Los Alamos National Laboratory (LANL); Lucas, Susan [U.S. Department of Energy, Joint Genome Institute; Copeland, A [U.S. Department of Energy, Joint Genome Institute; Pitluck, Sam [U.S. Department of Energy, Joint Genome Institute; Goodwin, Lynne A. [Los Alamos National Laboratory (LANL); Han, Cliff [Los Alamos National Laboratory (LANL); Tapia, Roxanne [Los Alamos National Laboratory (LANL); Brambilla, Evelyne-Marie [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Potter, Gabriele [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Land, Miriam L [ORNL; Ivanova, N [U.S. Department of Energy, Joint Genome Institute; Rohde, Manfred [HZI - Helmholtz Centre for Infection Research, Braunschweig, Germany; Goker, Markus [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Detter, J. Chris [U.S. Department of Energy, Joint Genome Institute; Li, Wen-Jun [Yunnan University, Kunming, China; Kyrpides, Nikos C [U.S. Department of Energy, Joint Genome Institute; Woyke, Tanja [U.S. Department of Energy, Joint Genome Institute

    2012-01-01

    Saccharomonospora marina Liu et al. 2010 is a member to the genomically so far poorly characterized genus Saccharomonospora in the family Pseudonocardiaceae. Members of the genus Sacharomonospora are of interest because they originate from diverse habitats, such as leaf litter, manure, compost, surface of peat, moist, over-heated grain, and ocean sediment, where they might play a role in the primary degradation of plant material by attacking hemicellulose. Organisms belonging to the genus are usually Gram-positive staining, non-acid fast, and classify among the actinomycetes. Next to S. viridis and S. azurea, S. marina is the third member in the genus Saccharomonospora for with a completely sequenced (permanent draft status) type strain genome will be published. Here we describe the features of this organism, together with the complete genome sequence, and annotation. The 5,965,593 bp long chromosome with its 5,727 protein-coding and 57 RNA genes was sequenced as part of the DOE funded Community Sequencing Program (CSP) 2010 at the Joint Genome Institute (JGI).

  13. A biliverdin-binding cyanobacteriochrome from the chlorophyll d-bearing cyanobacterium Acaryochloris marina.

    Science.gov (United States)

    Narikawa, Rei; Nakajima, Takahiro; Aono, Yuki; Fushimi, Keiji; Enomoto, Gen; Ni-Ni-Win; Itoh, Shigeru; Sato, Moritoshi; Ikeuchi, Masahiko

    2015-01-22

    Cyanobacteriochromes (CBCRs) are linear tetrapyrrole-binding photoreceptors in cyanobacteria that absorb visible and near-ultraviolet light. CBCRs are divided into two types based on the type of chromophore they contain: phycocyanobilin (PCB) or phycoviolobilin (PVB). PCB-binding CBCRs reversibly photoconvert at relatively long wavelengths, i.e., the blue-to-red region, whereas PVB-binding CBCRs reversibly photoconvert at shorter wavelengths, i.e., the near-ultraviolet to green region. Notably, prior to this report, CBCRs containing biliverdin (BV), which absorbs at longer wavelengths than do PCB and PVB, have not been found. Herein, we report that the typical red/green CBCR AM1_1557 from the chlorophyll d-bearing cyanobacterium Acaryochloris marina can bind BV almost comparable to PCB. This BV-bound holoprotein reversibly photoconverts between a far red light-absorbing form (Pfr, λmax = 697 nm) and an orange light-absorbing form (Po, λmax = 622 nm). At room temperature, Pfr fluoresces with a maximum at 730 nm. These spectral features are red-shifted by 48~77 nm compared with those of the PCB-bound domain. Because the absorbance of chlorophyll d is red-shifted compared with that of chlorophyll a, the BV-bound AM1_1557 may be a physiologically relevant feature of A. marina and is potentially useful as an optogenetic switch and/or fluorescence imager.

  14. Effects of filamentous macroalgae mats on growth and survival of eelgrass, Zostera marina, seedlings

    DEFF Research Database (Denmark)

    Rasmussen, Jonas; Olesen, Birgit; Krause-Jensen, Dorte

    2012-01-01

    A laboratory experiment was conducted to assess the effect of filamentous algae mats on the performance of seedlings of the eelgrass, Zostera marina. The seedlings were covered by three levels (3, 6 and 9 cm) of natural (Chaetomorpha linum) and imitation algae mats and it was hypothesized that th...... enriched sediments and longer duration of exposure than applied here may result in oxygen depletion thereby intensifying the negative effects of light attenuation by algal mats on seedling performance.......A laboratory experiment was conducted to assess the effect of filamentous algae mats on the performance of seedlings of the eelgrass, Zostera marina. The seedlings were covered by three levels (3, 6 and 9 cm) of natural (Chaetomorpha linum) and imitation algae mats and it was hypothesized...... that the effects of the natural algae on seedling growth may be more severe because of the metabolic demands of the algae. Results show that coverage by both C. linum and imitation algae significantly reduced seedling growth and increased allocation of resources to above ground tissues. No clear effects of algae...

  15. Endotoxin Structures in the Psychrophiles Psychromonas marina and Psychrobacter cryohalolentis Contain Distinctive Acyl Features

    Directory of Open Access Journals (Sweden)

    Charles R. Sweet

    2014-07-01

    Full Text Available Lipid A is the essential component of endotoxin (Gram-negative lipopolysaccharide, a potent immunostimulatory compound. As the outer surface of the outer membrane, the details of lipid A structure are crucial not only to bacterial pathogenesis but also to membrane integrity. This work characterizes the structure of lipid A in two psychrophiles, Psychromonas marina and Psychrobacter cryohalolentis, and also two mesophiles to which they are related using MALDI-TOF MS and fatty acid methyl ester (FAME GC-MS. P. marina lipid A is strikingly similar to that of Escherichia coli in organization and total acyl size, but incorporates an unusual doubly unsaturated tetradecadienoyl acyl residue. P. cryohalolentis also shows structural organization similar to a closely related mesophile, Acinetobacter baumannii, however it has generally shorter acyl constituents and shows many acyl variants differing by single methylene (-CH2- units, a characteristic it shares with the one previously reported psychrotolerant lipid A structure. This work is the first detailed structural characterization of lipid A from an obligate psychrophile and the second from a psychrotolerant species. It reveals distinctive structural features of psychrophilic lipid A in comparison to that of related mesophiles which suggest constitutive adaptations to maintain outer membrane fluidity in cold environments.

  16. Endotoxin structures in the psychrophiles Psychromonas marina and Psychrobacter cryohalolentis contain distinctive acyl features.

    Science.gov (United States)

    Sweet, Charles R; Alpuche, Giancarlo M; Landis, Corinne A; Sandman, Benjamin C

    2014-07-09

    Lipid A is the essential component of endotoxin (Gram-negative lipopolysaccharide), a potent immunostimulatory compound. As the outer surface of the outer membrane, the details of lipid A structure are crucial not only to bacterial pathogenesis but also to membrane integrity. This work characterizes the structure of lipid A in two psychrophiles, Psychromonas marina and Psychrobacter cryohalolentis, and also two mesophiles to which they are related using MALDI-TOF MS and fatty acid methyl ester (FAME) GC-MS. P. marina lipid A is strikingly similar to that of Escherichia coli in organization and total acyl size, but incorporates an unusual doubly unsaturated tetradecadienoyl acyl residue. P. cryohalolentis also shows structural organization similar to a closely related mesophile, Acinetobacter baumannii, however it has generally shorter acyl constituents and shows many acyl variants differing by single methylene (-CH2-) units, a characteristic it shares with the one previously reported psychrotolerant lipid A structure. This work is the first detailed structural characterization of lipid A from an obligate psychrophile and the second from a psychrotolerant species. It reveals distinctive structural features of psychrophilic lipid A in comparison to that of related mesophiles which suggest constitutive adaptations to maintain outer membrane fluidity in cold environments.

  17. Incidencias del fortalecimiento de la Marina Mercante Colombiana en la competitividad logística nacional

    Directory of Open Access Journals (Sweden)

    Diego Edison Cabuya Padilla

    2017-02-01

    Full Text Available La presente investigación se enmarca en la línea de investigación “Gestión Marítima, Fluvial y Portua-ria” específicamente en el tema de “Desarrollo de la Marina Mercante”, del grupo de investigación de la Facultad de Administración de la Escuela Naval de Cadetes “Almirante Padilla”. El propósito de la investigación fue determinar de qué manera afecta el fortalecimiento de la marina mercante a la com-petitividad logística de Colombia; estudiando los índices de competitividad mediante una revisión y análisis de la bibliografía, teoría, encuestas y datos especializados de los temas en cuestión, y un análisis estadístico de los datos de los índices de desempeño logístico a nivel internacional

  18. Potential effects of the invasive species Gracilaria vermiculophylla on Zostera marina metabolism and survival.

    Science.gov (United States)

    Martínez-Lüscher, Johann; Holmer, Marianne

    2010-06-01

    The potential threat to seagrasses of the invasive algae, Gracilaria vermiculophylla was assessed through metabolic indicators under experimental conditions. Net leaf photosynthesis (LNP) and dark respiration (LDR) were measured from leaf segments of Zostera marina shoots under different loads of G. vermiculophylla (control, low 2.2kg FW m(-2) and high 4kg FW m(-2)) in mesocosm experiments separated in tanks at four temperatures (19, 23.5, 26 and 30 degrees C). LNP decreased in the presence of the high density G. vermiculophylla mat (25% on average), being the most severe reductions at 30 degrees C (35% less in high). LDR did not respond significantly to differences in algal biomass, whereas a progressive increase was found with increasing temperatures (3.4 times higher at 30 degrees C than at 19 degrees C). Sulphide in porewater was measured weekly in order clarify the role of sediment conditions on seagrass metabolism, and increased both with algal biomass (29% in high) and temperature (from 0.5mM at 26 degrees C to 2.6mM at 30 degrees C), but changes in LNP and LDR were not correlated with sulphide concentrations. Seagrass survival rates showed decreasing trend with algal biomass at all the temperatures (from 74% to 21% survival). G. vermiculophylla showed harmful effects on Z. marina metabolism and survival with synergistic effects of temperature suggesting greater impact of invasive species under future higher water temperatures.

  19. Bohemia as the Homeland of the Soul in the Letters of Marina Tsvetaeva to Anna Teskova

    Directory of Open Access Journals (Sweden)

    Massimo Tria

    2014-03-01

    Full Text Available The article examines the letters sent by Marina Tsvetaeva to the translator and public figure Anna Teskova, who was one of the few poet’s Czech intimate friends, whom she met during her stay in Czechoslovakia (1922-1925. In the first part of the paper the Author focuses on the echoes of Prague and its culture in this specific correspondence, trying to ascertain to which extent Marina Tsvetaeva was acquainted with the cultural world she lived in: the lack of knowledge of the Czech language and the limited relationships with Prague intellectuals didn’t allow her to get a deep insight of the Czech cultural milieu. The textual analysis of the letters, though, on the basis of key concepts as “родной”, “родина”, “честь”, confirms the presence of a persistent feeling of Prague-homesickness in Tsvetaeva’s psycho- logical world, which brought her to develop an alternative model of Homeland, substitute to her native Russia and to Germany, which disappointed her after the rise of the Nazi Regime. Through this unique correspondence (including the first part of her Verses dedicated to Bohemia we gain the certainty that Tsvetaeva discovered in Prague and in the Czech lands an idealized model of a pacific, beautiful and faithful Homeland.

  20. The Existing Condition of Mangrove Region of Avicenia marina, Its: Distribution and Functional Transformation

    Directory of Open Access Journals (Sweden)

    Ahmad Herison

    2014-04-01

    Full Text Available Mangrove ecosystem existence is important for environment and other organisms because of its ecological and economical values, so that management and preservation of mangrove ecosystem are needed. The purpose of this research was to determine the existing condition of mangrove, both its distribution and its functional transformation in Indah Kapuk Coastal Area. Avicennia marina becomes important as wave attenuation, a form of abrasion antidote. Transect-Square and Spot-Check methods were used to determine the existing condition of A.marina mangrove forests. Autocad program, coordinate converter, Google Earth, Google Map, and Arc View were applied in process of making mangrove distribution map. In western of research location exactly at Station 1 and Station 2, the density value of mangrove was 450 and 825 tree ha-1, respectively with sparse category because they were contaminated by waste and litter. In eastern of research location namely Station 3, Station 4, and Station 5 the mangroves grow well with density value of 650 (sparse, 1,500 (very dense, and 1,200 tree ha-1 (fair, respectively, eventhough the contamination still happened. The mangrove forests around the stations do not function as wave attenuation because there were many waterfront constructions which have replaced the function of mangrove forests to damp the wave. In short, it can be stated that the mangrove's function has changed in a case of wave attenuation. The function of mangrove forests is not determined by mangrove forest density but it is determined by mangrove's free position.

  1. Far-red light promotes biofilm formation in the cyanobacterium Acaryochloris marina.

    Science.gov (United States)

    Hernández-Prieto, Miguel A; Li, Yaqiong; Postier, Bradley L; Blankenship, Robert E; Chen, Min

    2017-10-20

    Light quantity and quality promotes ecological-niche differentiation of photosynthetic organisms. The existence of cyanobacteria capable of performing photosynthesis using red-shifted chlorophylls, chlorophyll d and f, reduces competition between species in light-limiting environments, and permits them to thrive in niches enriched in far-red light. We examined global transcriptome changes due to changing the culture light conditions in Acaryochloris marina, a chlorophyll d-containing cyanobacterium. We identified the functional category of 'photosynthesis' as the most down-regulated and the category of 'cell wall/membrane biogenesis' as the most up-regulated through a functional enrichment analysis of genes differentially expressed. Within the category of 'cell wall/membrane biogenesis', genes encoding glycosysltransferases accumulated the most in response to far-red light. Further experimental results confirmed that cells grown under far-red light form biofilms with a significantly increased adherence compared to cells grown under white light. Taken together, these results indicate that Acaryochloris marina shifts its lifestyle from a planktonic state under white light to an immobilized state under far-red light. © 2017 Society for Applied Microbiology and John Wiley & Sons Ltd.

  2. Evaluation of antiherpetic activity of crude extract and fractions of Avicenna marina, in vitro.

    Science.gov (United States)

    Behbahani, Mandana; Zadeh, Mehrnaz Shanehsaz; Mohabatkar, Hassan

    2013-03-01

    This study was carried out to check antiherpetic substances of crude methanol leaf extract of Avicenna marina and its column chromatographic fractions. Herpes simplex virus 2 (HSV-2) is a harmful pathogen especially in highly susceptible individuals. The antiherpetic activity of crude methanol extract and sub-fractions was performed in different concentrations (20, 2, 0.2, and 0.02μg/ml) by use of plaque-forming unit (PFU) assay and real time polymerase chain reaction (PCR) assay. The most active fraction analyzed by NMR contained luteolin 7-O-methylether 3'-O-beta-d-glucoside (LMEG). The other active fraction was detected by HPLC as luteolin. The apparent effective concentrations for 50% plaque reduction (EC50) of crude methanol extract, LMEG, luteolin and ACV were 10, 5, 16.6 and 2.97μg/ml, respectively. The three extracts showed no cytotoxic effect on Vero cell line at concentrations of 32μg/ml or below. According to the consequences of time-of-addition studies, antiherpetic compound LMEG exerted an inhibitory effect on the early stage of HSV-2 infection during which it was added. In conclusion, LMEG isolated from A. marina could probably inhibit HSV attachment to the cell membrane and its entry into the cell. Copyright © 2013 Elsevier B.V. All rights reserved.

  3. Nutrient Dynamics in an Avicennia marina (Forsk. Vierh., Mangrove Forest in Vamleshwar, Gujarat, India

    Directory of Open Access Journals (Sweden)

    Isaiah Nirmal KUMAR

    2011-03-01

    Full Text Available The study was carried out to determine the nutrient budget of plants, sediments and nutrient dynamics in an Avicennia marina (Forsk. Vierh., dominated forest in Vamleshwar near Narmada estuary, West Coast of Gujarat for a period of one year from November 2008 to October 2009. The average tree height of the mangrove is 1.5 to 2 m without much vertical stratification. Allometric methodology was used to measure the biomass, and yield a figure of 86.47 t ha-1 and the litter fall rate amounted to 2.9 t ha-1. Nutrient stocks of N, P and K in this mangrove were 137.05, 14.38 and 241.29 kg ha-1, with an annual accumulation of 55.74, 12.38 and 83.94 kg ha-1, and an annual return of 51.30, 10.83 and 13.52 kg ha-1, respectively, in the form of litter. The annual uptake for N, P and K were 61.04, 14.28 and 97.46 kg ha-1, and turnover rates of N, P and K were estimated at 3, 6 and 14 years, respectively, for the study period. Flow coefficients, which reveal the dynamic processes of nutrients between mangrove plants and sediments, are also explained. The present study concluded that the A. marina dominated mangrove plantation is more efficient in nutrient use and conservation.

  4. Phenology and Growth dynamics of Avicennia marina in the Central Red Sea

    KAUST Repository

    Almahasheer, Hanan

    2016-11-28

    The formation of nodes, stem elongation and the phenology of stunted Avicennia marina was examined in the Central Red Sea, where Avicennia marina is at the limit of its distribution range and submitted to extremely arid conditions with salinity above 38 psu and water temperature as high as 35° C. The annual node production was rather uniform among locations averaging 9.59 node y−1, which resulted in a plastocron interval, the interval in between production of two consecutive nodes along a stem, of 38 days. However, the internodal length varied significantly between locations, resulting in growth differences possibly reflecting the environmental conditions of locations. The reproductive cycle lasted for approximately 12 months, and was characterized by peak flowering and propagule development in November and January. These phenological observations provide a starting point for research and restoration programs on the ecology of mangroves in the Central Red Sea, while the plastochrone index reported here would allow calculations of the growth and production of the species from simple morphological measurements.

  5. An effective seed protection method for planting Zostera marina (eelgrass) seeds: Implications for their large-scale restoration.

    Science.gov (United States)

    Zhang, Pei-Dong; Fang, Chao; Liu, Jie; Xu, Qiang; Li, Wen-Tao; Liu, Yan-Shan

    2015-06-15

    We describe an innovative method of planting Zostera marina (eelgrass) seeds in which hessian bags filled with high-silted sediments are used as a seed protecting device. Here, we evaluated the effectiveness of the method through a field seed-sowing experiment over a three year period. The suitable seed planting density required by the seeds of Z. marina in this method was also investigated. In the spring following seed distribution, seedling establishment rate of Z. marina subjected to different seed densities of 200-500seedsbag(-1) ranged from 16% to 26%. New eelgrass patches from seed were fully developed and well maintained after 2-3years following distribution. The seed planting density of 400seedsbag(-1) may be the most suitable for the establishment of new eelgrass patches. Our results demonstrate that seed-based restoration can be an effective restoration tool and the technique presented should be considered for future large-scale Z. marina restoration projects. Copyright © 2015 Elsevier Ltd. All rights reserved.

  6. EFFECTS OF RECREATIONAL CLAM HARVESTING ON EELGRASS (ZOSTERA MARINA) AND ASSOCIATED INFAUNAL INVERTEBRATES: IN SITU MANIPULATIVE EXPERIMENTS

    Science.gov (United States)

    The effect of recreational clam harvesting on eelgrass (Zostera marina) was experimentally tested by raking or digging for clams in experimental 1-m2 plots located in a Yaquina Bay (Newport, OR) eelgrass meadow. After three monthly treatments, eelgrass measures of biomass, prima...

  7. Cytotoxic Effects of Vicicitus globosus (Class Dictyochophyceae and Chattonella marina (Class Raphidophyceae on Rotifers and Other Microalgae

    Directory of Open Access Journals (Sweden)

    Fook Hoe Chang

    2015-06-01

    Full Text Available Cultures of Vicicitus globosus (previously Chattonella globosa and Chattonella marina, established during the 2010 fish kill event in Mahanga Bay, Wellington Harbour, are confirmed to be cytotoxic. The aggregate potency of lipophilic cell extracts of each species were evaluated using three species each of flagellates, dinoflagellates and diatoms, and a rotifer as test organisms. The cell extract of V. globosus destroyed cells of all nine microalgae in a matter of a few minutes to less than 15 min, while that of C. marina, destroyed all species over 10 to 30 min. The lipophilic extract of V. globosus caused partial disintegration of both theca wall and cytoplasm of cells of Alexandrium catenella in a matter of minutes. This effect, however, was not observed in cells of A. catenella exposed to that of C. marina. Tests conducted on rotifers showed similar fast-acting trends, with animals exposed to a cell extract of V. globosus died in a much shorter time (Lt50 = 80 min than those exposed to that of C. marina (20 h.

  8. Significance of salinity and silicon levels for growth of a formerly estuarine eelgrass (Zostera marina) population (Lake Grevelingen, the Netherlands)

    NARCIS (Netherlands)

    Kamermans, P.; Hemminga, M.A.; De Jong, D.J.

    1999-01-01

    Since the early 1980s, the eelgrass, Zostera marina L., population in the saline Lake Gevelingen, The Netherlands, is rapidly declining. An earlier study, in which long-term data on eelgrass coverage in this former estuary were correlated with several environmental variables, showed only one

  9. Combined toxicity of cadmium and copper in Avicennia marina seedlings and the regulation of exogenous jasmonic acid.

    Science.gov (United States)

    Yan, Zhongzheng; Li, Xiuzhen; Chen, Jun; Tam, Nora Fung-Yee

    2015-03-01

    Seedlings of Avicennia marina were exposed to single and combined metal treatments of cadmium (Cd) and copper (Cu) in a factorial design, and the combined toxicity of Cu and Cd was tested. The effects of the exogenous jasmonic acid (JA) on chlorophyll concentration, lipid peroxidation, Cd and Cu uptake, antioxidative capacity, endogenous JA concentration, and type-2 metallothionein gene (AmMT2) expression in seedlings of A. marina exposed to combined metal treatments were also investigated. A binary mixture of low-dose Cd (9 µmolL(-1)) and high-dose Cu (900 µmolL(-1)) showed toxicity to the seedlings, indicated by the significant augmentation in leaf malondialdehyde (MDA) and reduction in leaf chlorophylls. The toxicity of the combined metals was significantly alleviated by the addition of exogenous JA at 1 µmolL(-1), and the chlorophyll and MDA contents were found to be restored to levels comparable to those of the control. Compare to treatment with Cd and Cu only, 1 and 10 µmolL(-1) JA significantly enhanced the ascorbate peroxidase activity, and 10 µmolL(-1) JA significantly decreased the uptake of Cd in A. marina leaves. The relative expression of leaf AmMT2 gene was also significantly enhanced by 1 and 10 µmolL(-1) JA, which helped reduce Cd toxicity in A. marina seedlings. Copyright © 2014 Elsevier Inc. All rights reserved.

  10. Sediment Properties as Important Predictors of Carbon Storage in Zostera marina Meadows: A Comparison of Four European Areas.

    Directory of Open Access Journals (Sweden)

    Martin Dahl

    Full Text Available Seagrass ecosystems are important natural carbon sinks but their efficiency varies greatly depending on species composition and environmental conditions. What causes this variation is not fully known and could have important implications for management and protection of the seagrass habitat to continue to act as a natural carbon sink. Here, we assessed sedimentary organic carbon in Zostera marina meadows (and adjacent unvegetated sediment in four distinct areas of Europe (Gullmar Fjord on the Swedish Skagerrak coast, Askö in the Baltic Sea, Sozopol in the Black Sea and Ria Formosa in southern Portugal down to ~35 cm depth. We also tested how sedimentary organic carbon in Z. marina meadows relates to different sediment characteristics, a range of seagrass-associated variables and water depth. The seagrass carbon storage varied greatly among areas, with an average organic carbon content ranging from 2.79 ± 0.50% in the Gullmar Fjord to 0.17 ± 0.02% in the area of Sozopol. We found that a high proportion of fine grain size, high porosity and low density of the sediment is strongly related to high carbon content in Z. marina sediment. We suggest that sediment properties should be included as an important factor when evaluating high priority areas in management of Z. marina generated carbon sinks.

  11. APPARENT LACK OF VESICULAR-ARBUSCULAR MYCORRHIZA (VAM) IN SEAGRASSES ZOSTERA MARINA L. AND THALASSIA TESTUDIUM BANKS EX KONIG

    Science.gov (United States)

    We examined two populations of Zostera marina L. and one of Thalassia testudinum Banks ex Konig for presence of vesicular-arbuscular mycorrhiza (VAM). None of these plants showed any VAM colonization. In addition, we were unable to find any literature references on the presence o...

  12. A tale of two seagrasses: Comparing the science and management of Zostera marina and Zostera japonica in the Pacific Northwest

    Science.gov (United States)

    On the Pacific coast of North America, at least two congeners of Zostera occur: native Zostera marina, and introduced, Z. japonica. Z. japonica is considered “invasive” and therefore, ecologically and economically harmful by some, while others consider it benign or perhaps benef...

  13. Feeding by the heterotrophic dinoflagellate Oxyrrhis marina on the red-tide raphidophyte Heterosigma akashiwo: a potential biological method to control red tides using mass-cultured grazers.

    Science.gov (United States)

    Jeong, Hae Jin; Kim, Jae Seong; Yoo, Yeong Du; Kim, Seong Taek; Kim, Tae Hoon; Park, Myung Gil; Lee, Chang Hoon; Seong, Kyeong Ah; Kang, Nam Seon; Shim, Jae Hyung

    2003-01-01

    As part of the development of a method to control the outbreak and persistence of red tides using mass-cultured heterotrophic protist grazers, we measured the growth and ingestion rates of cultured Oxyrrhis marina (a heterotrophic dinoflagellate) on cultured Heterosigma akashiwo (a raphidophyte) in bottles in the laboratory and in mesocosms (ca. 60 liter) in nature, and those of the cultured grazer on natural populations of the red-tide organism in mesocosms set up in nature. In the bottle incubation, specific growth rates of O. marina increased rapidly with increasing concentration of cultured prey up to ca. 950 ng C ml(-1) (equivalent to 9,500 cells ml(-1)), but were saturated at higher concentrations. Maximum specific growth rate (mumax), KGR (prey concentration sustaining 0.5 mumax) and threshold prey concentration of O. marina on H. akashiwo were 1.43 d(-1), 104 ng C ml(-1), and 8.0 ng C ml(-1), respectively. Maximum ingestion and clearance rates of O. marina were 1.27 ng C grazer(-1) d(-1) and 0.3 microl grazer(-1) h(-1), respectively. Cultured O. marina grew well effectively reducing cultured and natural populations of H. akashiwo down to a very low concentration within 3 d in the mesocosms. The growth and ingestion rates of cultured O. marina on natural populations of H. akashiwo in the mesocosms were 39% and 40%, respectively, of those calculated based on the results from the bottle incubation in the laboratory, while growth and ingestion rates of cultured O. marina on cultured H. akashiwo in the mesocosms were 55% and 36%, respectively. Calculated grazing impact by O. marina on natural populations of H. akashiwo suggests that O. marina cultured on a large scale could be used for controlling red tides by H. akashiwo near aquaculture farms that are located in small ponds, lagoons, semi-enclosed bays, and large land-aqua tanks to which fresh seawater should be frequently supplied.

  14. The transcriptome of the novel dinoflagellate Oxyrrhis marina (Alveolata: Dinophyceae: response to salinity examined by 454 sequencing

    Directory of Open Access Journals (Sweden)

    Montagnes David JS

    2011-10-01

    Full Text Available Abstract Background The heterotrophic dinoflagellate Oxyrrhis marina is increasingly studied in experimental, ecological and evolutionary contexts. Its basal phylogenetic position within the dinoflagellates make O. marina useful for understanding the origin of numerous unusual features of the dinoflagellate lineage; its broad distribution has lent O. marina to the study of protist biogeography; and nutritive flexibility and eurytopy have made it a common lab rat for the investigation of physiological responses of marine heterotrophic flagellates. Nevertheless, genome-scale resources for O. marina are scarce. Here we present a 454-based transcriptome survey for this organism. In addition, we assess sequence read abundance, as a proxy for gene expression, in response to salinity, an environmental factor potentially important in determining O. marina spatial distributions. Results Sequencing generated ~57 Mbp of data which assembled into 7, 398 contigs. Approximately 24% of contigs were nominally identified by BLAST. A further clustering of contigs (at ≥ 90% identity revealed 164 transcript variant clusters, the largest of which (Phosphoribosylaminoimidazole-succinocarboxamide synthase was composed of 28 variants displaying predominately synonymous variation. In a genomic context, a sample of 5 different genes were demonstrated to occur as tandem repeats, separated by short (~200-340 bp inter-genic regions. For HSP90 several intergenic variants were detected suggesting a potentially complex genomic arrangement. In response to salinity, analysis of 454 read abundance highlighted 9 and 20 genes over or under expressed at 50 PSU, respectively. However, 454 read abundance and subsequent qPCR validation did not correlate well - suggesting that measures of gene expression via ad hoc analysis of sequence read abundance require careful interpretation. Conclusion Here we indicate that tandem gene arrangements and the occurrence of multiple transcribed

  15. Rhizobial strains isolated from nodules of Medicago marina in southwest Spain are abiotic-stress tolerant and symbiotically diverse.

    Science.gov (United States)

    Alías-Villegas, Cynthia; Cubo, M Teresa; Lara-Dampier, Victoria; Bellogín, Ramón A; Camacho, María; Temprano, Francisco; Espuny, M Rosario

    2015-10-01

    The isolation and characterisation of nitrogen-fixing root nodule bacteria from Medicago marina, a tolerant legume species, were studied in two areas from southwest Spain. A total of 30 out of 82 isolates with distinct ERIC-PCR fingerprints were analysed on the basis of molecular (PCR-RFLP of the 16S-23S rDNA intergenic spacer region (IGS) with two endonucleases, analysis of the 16S rDNA and symbiotic nodC gene sequences, plasmid profiles and SDS-PAGE of LPS, including the partial sequence of the housekeeping gene glnII and the symbiotic gene nodA of some representatives), physiological (utilisation of sole carbon sources, tolerance to antibiotics, NaCl, heavy metals, temperature and pH) and symbiotic parameters (efficacy on M. marina, M. minima, M. murex, M. orbicularis, M. polymorpha, M. sativa and M. truncatula). All the bacteria isolated from M. marina nodules belonged to Ensifer meliloti, except for one strain that belonged to E. medicae. To determine the nodulation range of M. marina, 10 different Ensifer species were tested for their ability to nodulate on this plant. E. kummerowiae CCBAU 71714 and the E. medicae control strain M19.1 were the only Ensifer species tested that developed nitrogen-fixing nodules on this plant. Most of the M. marina-nodulating strains showed tolerance to stress factors and all of them shared the presence of a gene similar to cadA, a gene that encodes for a PIB-type ATPase, which is a transporter belonging to the large superfamily of ATP-driven pumps involved in the transport of metals across cell membranes. Copyright © 2015 Elsevier GmbH. All rights reserved.

  16. Genome-wide transcriptomic responses of the seagrasses Zostera marina and Nanozostera noltii under a simulated heatwave confirm functional types.

    Science.gov (United States)

    Franssen, Susanne U; Gu, Jenny; Winters, Gidon; Huylmans, Ann-Kathrin; Wienpahl, Isabell; Sparwel, Maximiliane; Coyer, James A; Olsen, Jeanine L; Reusch, Thorsten B H; Bornberg-Bauer, Erich

    2014-06-01

    Genome-wide transcription analysis between related species occurring in overlapping ranges can provide insights into the molecular basis underlying different ecological niches. The co-occurring seagrass species, Zostera marina and Nanozostera noltii, are found in marine coastal environments throughout the northern hemisphere. Z. marina is often dominant in subtidal environments and subjected to fewer temperature extremes compared to the predominately intertidal and more stress-tolerant N. noltii. We exposed plants of both species to a realistic heat wave scenario in a common-stress-garden experiment. Using RNA-seq (~7million reads/library), four Z. marina and four N. noltii libraries were compared representing northern (Denmark) and southern (Italy) locations within the co-occurring range of the species' European distribution. A total of 8977 expressed genes were identified, of which 78 were directly related to heat stress. As predicted, both species were negatively affected by the heat wave, but showed markedly different molecular responses. In Z. marina the heat response was similar across locations in response to the heatwave at 26°C, with a complex response in functions related to protein folding, synthesis of ribosomal chloroplast proteins, proteins involved in cell wall modification and heat shock proteins (HSPs). In N. noltii the heat response markedly differed between locations, while HSP genes were not induced in either population. Our results suggest that as coastal seawater temperatures increase, Z. marina will disappear along its southern most ranges, whereas N. noltii will continue to move north. As a consequence, sub- and intertidal habitat partitioning may weaken in more northern regions because the higher thermal tolerance of N. noltii provides a competitive advantage in both habitats. Although previous studies have focused on HSPs, the present study clearly demonstrates that a broader examination of stress related genes is necessary. Copyright

  17. Avicennia marina

    African Journals Online (AJOL)

    SAM

    2014-04-23

    Apr 23, 2014 ... More recently, Song et al. (2009) reported a decline in the activity of cytochrome c oxidase in recalcitrant Antiaris toxicaria axes and orthodox Zea ... Smith et al., 2001; Huang et al., 2009; Ntuli et al., 2011). It has been proposed .... Activity was determined as the decomposition of H2O2 by the decrease in ...

  18. Avicennia marina

    African Journals Online (AJOL)

    SAM

    2014-04-23

    Apr 23, 2014 ... 1996; Leprince and Hoekstra, 1998; Ntuli et al., 2011) and hence results in metabolic imbalance (Finch-Savage et al., 1993; reviewed by Vertucci and Farrant, 1995;. Leprince et al., 2000; Ntuli et al., 2011). Of a particular interest in this regard, is respiratory metabolism. For instance, it has been shown that ...

  19. Characterisation of Major Histocompatibility Complex Class I in the Australian Cane Toad, Rhinella marina

    Science.gov (United States)

    Lillie, Mette; Shine, Richard; Belov, Katherine

    2014-01-01

    The Major Histocompatibility Complex (MHC) class I is a highly variable gene family that encodes cell-surface receptors vital for recognition of intracellular pathogens and initiation of immune responses. The MHC class I has yet to be characterised in bufonid toads (Order: Anura; Suborder: Neobatrachia; Family: Bufonidae), a large and diverse family of anurans. Here we describe the characterisation of a classical MHC class I gene in the Australian cane toad, Rhinella marina. From 25 individuals sampled from the Australian population, we found only 3 alleles at this classical class I locus. We also found large number of class I alpha 1 alleles, implying an expansion of class I loci in this species. The low classical class I genetic diversity is likely the result of repeated bottleneck events, which arose as a result of the cane toad's complex history of introductions as a biocontrol agent and its subsequent invasion across Australia. PMID:25093458

  20. Rezension zu: Marina Caffiero (Hg.: Rubare le anime. Roma: Viella 2008

    Directory of Open Access Journals (Sweden)

    Rita Unfer Lukoschik

    2009-07-01

    Full Text Available In den Akten des päpstlichen Staatsarchivs und der römischen jüdischen Gemeinde finden sich zeitgenössische Zeugnisse für die im päpstlichen Rom vom 16. bis zum 19. Jahrhundert nicht seltene Zwangskonvertierung jüdischer Mädchen und Frauen zum Katholizismus. Eines der wertvollsten Dokumente dieser Konversionspraxis liegt nunmehr in einer kommentierten Neuedition vor: das Tagebuch der 1749 sich erfolgreich der versuchten Zwangskonvertierung widersetzenden 18-jährigen Anna del Monte. In ihrer Einleitung ordnet die Herausgeberin Marina Caffiero dieses außergewöhliche Einzelschicksal in die zeitgleich und parallel laufenden Assimilations- und Emanzipationsprozesse ein, die sich in der mit aufklärerischem Gedankengut ‚infizierten‘ jüdischen Oberschicht Roms im 18. Jahrhundert abzeichneten.

  1. Contaminación marina por petróleo en aguas costeras ecuatorianas

    OpenAIRE

    Valencia, M.; Trejos de Suescum, R.

    1986-01-01

    El presente trabajo realiza un enfoque general acerca de la problemática de la contaminación marina por petróleo, el comportamiento de este contaminante en el mar, así como las fuentes más probables de contaminación por petróleo. Además se presenta un enfoque específico de la concentración y distribución de hidrocarburos del petróleo, disueltos y dispersos en aguas del mar, frente a las principales poblaciones costeras de las provincias del Guayas y el Oro, tomando en consideración dos muestr...

  2. Effects of filamentous macroalgae on growth and survival of eelgrass, Zostera marina, seedlings

    DEFF Research Database (Denmark)

    Rasmussen, Jonas; Krause-Jensen, Dorte; Olesen, Birgit

    Seedling survival and patch establishment is a major bottle-neck for eelgrass (Zostera marina) re-colonization as mortality rates among the young seedlings are high, even in areas where conditions should support survival. We here focus on the potential negative effects of drifting macroalgal mats...... on a 2-factorial laboratory experiment. Eelgrass seedlings were grown with three different heights and two different types of algae mats: Chaetomorpha linum and artificial macroalgae. The two types of mats were used to separate the physical and metabolic effects of algal presence. Concentrations...... and effects on seedling growth can thus be attributed to reduced light caused by shading of the algae. Generally, the seedlings are shown to be robust to short-term reductions in light availability, but further experiments are needed to elucidate the relative contributions of reduced light vs. anoxic...

  3. High ammonium availability amplifies the adverse effect of low salinity on eelgrass Zostera marina

    DEFF Research Database (Denmark)

    Villazán, Beatriz; Salo, Tiina Elina; Brun, Fernando G.

    2015-01-01

    enrichment was followed by an increase in pigments, photosynthesis and various growth variables and a decrease in stored carbon concentrations (sucrose and starch). Low salinity had an overall negative effect on plant fitness; pigment concentration, photosynthesis and growth were reduced while mortality......Climate change intensifies the frequency and intensity of rainfall events, which increases the discharge of freshwater and nutrients to coastal areas. This may lower salinity and increase nutrient availability and, thus, affect estuarine eelgrass populations. We studied the interactive effect...... of increasing NH4+ levels and low salinity on estuarine eelgrass Zostera marina, grown in microcosm at various combinations of NH4+ enrichment (0, 10 and 25 µM) and salinity (5, 12.5 and 20). Increasing NH4+ had a positive effect on eelgrass performance as long as salinity was kept at ambient level (20). N...

  4. Synergistic effects of altered salinity and temperature on estuarine eelgrass (Zostera marina) seedlings and clonal shoots

    DEFF Research Database (Denmark)

    Salo, Tiina Elina; Pedersen, Morten Foldager

    2014-01-01

    Salinity and temperature are among the most important factors determining eelgrass distribution and performance. Plants in estuarine environments experience large variations in both on a seasonal basis and exceptionally warm summers have caused massive die-backs of eelgrass in many areas. We...... investigated experimentally how different combinations of salinity and temperature affect the physiological performance of adult eelgrass (Zostera marina) shoots and seedlings. Plants were exposed to different combinations of salinity (salinity 5, 12.5 and 20) and temperature (15, 20 and 25 °C) in a 5-week...... aquarium experiment. Plants responded in general negatively to decreasing salinity and increasing temperature and the combination of high temperature and low salinity resulted in markedly higher mortality rates and lower leaf production when compared to plants held at more optimal combinations of salinity...

  5. Komposisi kimia substrat asosiasi Sonneratia alba Avicennia marina di Muara Sungai Lekok Kabupaten Pasuruan

    Directory of Open Access Journals (Sweden)

    Hani Sudarmanto

    1997-12-01

    Full Text Available The mangrove forest occurred in the north coast of Pasuruan Regency have not been reported about the vegetational and substrate compositions. The researches purpose were to analysis the vegetational composition and the chemical properties of the substrate. The result showed that this mangrove forest were dominated by association of Sonneratia alba-Avicennia marina. The result showed that soils had the natural reaction (pH 7.01-7.65, low in organic matter (2.18-4.27% and total nitrogen (0.48-0.51%, but high in total phosphorus (15.19-29.22 ppm and in exchangeable cation (Ca2+: 389.855-504.065 mg/100 gr, Mg2+: 409.522-441.400 mg/100 gr, K+: 228.900-390-435 mg/100 gr.

  6. Cerium Binding Activity of Pectins Isolated from the Seagrasses Zostera marina and Phyllospadix iwatensis

    Directory of Open Access Journals (Sweden)

    Valeri Kovalev

    2012-04-01

    Full Text Available Cerium binding activity of three different water soluble pectin compounds of different origin was studied in a batch sorption system. The Langmuir, Freundlich and BET sorption models were adopted to describe the binding reactions between metal ions and pectin molecules. The Langmuir model provided the best fit. Within the pH range from 4.0 to 6.0, the largest amount of the cerium ions was bound by pectin isolated from the seagrass Phylospadix iwatensis in comparison to pectin extracted from the seagrass Zostera marina and pectin obtained from citrus peel (commercial grade. The Langmuir constants were also highest for the pectin samples isolated from the seagrass P. iwatensis. The results obtained from this study suggest that pectin is a prospective source for the development of radioisotope-removing pharmaceuticals.

  7. Photosynthetic carbon fixation pathways in Zostera marina and three Florida seagrasses

    Energy Technology Data Exchange (ETDEWEB)

    Beer, S.; Wetzel, R.G.

    1982-06-01

    The photosynthetic carbon fixation pathways of four seagrass species, Zostera marina L. from Alaska and Thalassia testudinum Banks ex Konig, Syringodium filiforme Kutz. and Halodule wrightii Aschers. from the Gulf of Mexico, were investigated with a /sup 14/C pulse-chase technique. All species were found to be principally of the C/sub 3/ type. However, Thalassia and Halodule had higher initial incorporation rates into organic acids than is typical for terrestrial C/sub 3/ plants. Of 11 seagrass species investigated thus far for C/sub 3/ or C/sub 4/ metabolism using this technique, 10 were found to be principally of the C/sub 3/ type while only one exhibited C/sub 4/ metabolism.

  8. Cerium binding activity of pectins isolated from the seagrasses Zostera marina and Phyllospadix iwatensis.

    Science.gov (United States)

    Khotimchenko, Yuri; Khozhaenko, Elena; Kovalev, Valeri; Khotimchenko, Maxim

    2012-04-01

    Cerium binding activity of three different water soluble pectin compounds of different origin was studied in a batch sorption system. The Langmuir, Freundlich and BET sorption models were adopted to describe the binding reactions between metal ions and pectin molecules. The Langmuir model provided the best fit. Within the pH range from 4.0 to 6.0, the largest amount of the cerium ions was bound by pectin isolated from the seagrass Phylospadix iwatensis in comparison to pectin extracted from the seagrass Zostera marina and pectin obtained from citrus peel (commercial grade). The Langmuir constants were also highest for the pectin samples isolated from the seagrass P. iwatensis. The results obtained from this study suggest that pectin is a prospective source for the development of radioisotope-removing pharmaceuticals.

  9. Antibacterial potential of biosynthesised silver nanoparticles using Avicennia marina mangrove plant

    Science.gov (United States)

    Gnanadesigan, M.; Anand, M.; Ravikumar, S.; Maruthupandy, M.; Syed Ali, M.; Vijayakumar, V.; Kumaraguru, A. K.

    2012-06-01

    The present study was aimed to identify the antibacterial potential of biosynthesised silver nanoparticles using different plant parts (leaves, bark and root) of Avicenna marina mangrove plant. Of the selected three different parts, the leaf extract showed the maximum synthesis of silver nanoparticles. The in vitro antibacterial assay (100 μg disk-1 concentration) showed the results of maximum zone of inhibition with the E. coli (18.40 ± 0.97 mm), and minimum (10.87 ± 1.33 mm) zone of inhibition with S. aureus but the concentrations of MIC and MBC values ranged between 6.25 and 50.0 μg ml-1 between the selected bacterial strains. The FTIR results of most potent leaf extract-synthesized silver nanoparticles showed the prominent peaks (620.967; 1,061.02; 1,116.58; 1,187.94; 1,280.50; 1,353.79; 1,384.64; 1,598.50; 1,629.56; 2,854.14 and 2,927.42) in different ranges. Further, the results of XRD analysis showed the 2 θ intense values (38.11 and 70.57) within the ranges of Bragg's reflection. In addition, the AFM analysis showed the results of particle sizes (71-110 nm), particle roughness (11.8 nm), maximum height of the particle roughness (111.8 nm), and average maximum height of the particle roughness (57.5 nm). It can be concluded from the present findings that, the biosynthesis of silver nanoparticles from the leaf extract of A. marina can be used as potential antibacterial agents.

  10. Sport and Tourism: a potentially conflictual relationship. The case of Marinas in Tenerife

    Directory of Open Access Journals (Sweden)

    Manuel Eduardo González Ramallal

    2010-01-01

    Full Text Available El artículo se centra en los conflictos que pueden surgir en el ámbito del deporte y el turismo partiendo del estudio de un caso centrado en dos puertos deportivos de la isla de Tenerife (España. A través de una aproximación cualitativa, se analizó el discurso de los participantes en siete actividades deportivas marinas diferentes. Frente a la tradicional visión optimista en torno a la relación entre deporte y turismo, los resultados de la investigación muestran que pueden surgir conflictos si ésta no se gestiona adecuadamente. Los conflictos pueden darse a tres niveles. En primer lugar, a nivel de práctica deportiva, donde los problemas pueden surgir entre las diferentes actividades deportivas que se dan en torno a los puertos deportivos. En segundo lugar, entre los diferentes grupos sociales que usan los puertos deportivos. En tercer lugar, a nivel turístico, los conflictos pueden surgir entre el modelo de desarrollo turístico y el uso que se le da a las marinas como espacios deportivos. El artículo concluye enfatizando las cuestiones clave a considerar para gestionar adecuadamente la relación deporte y turismo, tales como las de la imagen de marca asociada tanto a los deportes como a los destinos turísticos, así como los grupos sociales que habitualmente practican un deporte o visitan un destino.

  11. Photoacclimatory Responses of Zostera marina in the Intertidal and Subtidal Zones.

    Directory of Open Access Journals (Sweden)

    Sang Rul Park

    Full Text Available Photoacclimatory responses of the seagrass Zostera marina in the intertidal and subtidal zones were investigated by measuring chlorophyll a fluorescence parameters, photosynthetic pigments, leaf δ13C values, and shoot morphology in two bay systems. Intertidal plants had higher carotenoid concentrations than subtidal plants to avoid photodamage under excess light conditions during the day. The maximum relative electron transport rate (rETRmax and minimum saturation irradiance (Ek of the intertidal plants were higher than those of the subtidal plants, whereas photosynthetic efficiency (α and maximum quantum yield (Fv/Fm were higher in subtidal plants. The intertidal plants also had significantly greater Stern-Volmer non-photochemical quenching (NPQ than that of the subtidal plants. These results suggest that the subtidal plants photoacclimated to use limited light more efficiently, and the intertidal plants exhibited photosynthetic responses to minimize photodamage at excess irradiance. The δ13C values of leaf tissues were more negative in the intertidal plants than those in the subtidal plants, suggesting that the intertidal plants used atmospheric or dissolved CO2 for photosynthesis during emersion. Effective quantum yield (ΔF/Fm´ in the intertidal plants decreased more slowly after emersion than that in the subtidal plants, indicating higher desiccation tolerance of the intertidal plants. The intertidal plants also recovered more rapidly from desiccation damage than the subtidal plants, suggesting photosynthetic adaptation to desiccation stress. The photosynthetic plasticity of Z. marina in response to variable environmental conditions most likely allows this species to occur in the intertidal and subtidal zones.

  12. Wild Cane Toads (Rhinella marina) Expel Foreign Matter from the Coelom via the Urinary Bladder in Response to Internal Injury, Endoparasites and Disease: e0134036

    National Research Council Canada - National Science Library

    Crystal Kelehear; Hugh I Jones; Benjamin A Wood; Richard Shine

    2015-01-01

      Dissections of >1,200 wild-caught cane toads (Rhinella marina) in tropical Australia confirm a laboratory report that anurans can expel foreign objects from the coelom by incorporating them into the urinary bladder...

  13. Wild cane toads (Rhinella marina) expel foreign matter from the coelom via the urinary bladder in response to internal injury, endoparasites and disease

    National Research Council Canada - National Science Library

    Kelehear, Crystal; Jones, Hugh I; Wood, Benjamin A; Shine, Richard

    2015-01-01

    Dissections of >1,200 wild-caught cane toads (Rhinella marina) in tropical Australia confirm a laboratory report that anurans can expel foreign objects from the coelom by incorporating them into the urinary bladder...

  14. Rhizosphere O2 dynamics in young Zostera marina and Ruppia maritima

    DEFF Research Database (Denmark)

    Jovanovic, Zeljko; Pedersen, Mia Østergaard; Larsen, Morten

    2015-01-01

    Zostera marina and Ruppia maritima often share the same habitat, but R. maritima appears more resistant to environmental stress. We investigated the impact of light intensity and water column O2 concentrations on radial oxygen loss (ROL), in young specimens of Z. marina and R. maritima. Planar...... optode imaging revealed that ROL of Z. marina was localized to the root tip, while R. maritima showed ROL along extensive root sections. The total root biomass of the 2 species was similar, but, while R. maritima had only 1 root, of which 33% of its length showed ROL, Z. marina had 2 to 5 individual...... roots, where only 2 to 3 exhibited O2 leakage, but then only at root tips. ROL resulted in an oxic volume of 4.26 ± 0.51 mm3 plant−1 for Z. marina and 5.39 ± 0.47 mm3 plant−1 for R. maritima (n = 3). ROL per plant at light saturation was 2.32 ± 0.30 and 2.89 ± 0.38 nmol h−1 for Z. marina and R. maritima...

  15. Orígenes familiares y carrera profesional de Julián de Arriaga, Secretario de Estado de Marina e Indias (1700-1776

    Directory of Open Access Journals (Sweden)

    María Baudot Monroy

    2004-01-01

    Full Text Available La Secretaria de Marina surgida de las reformas administrativas de Felipe V con la finalidad de ocuparse específicamente de los asuntos de la Marina, fue dirigida durante el siglo xviii por diez y seis secretarios de Estado de Marina. Actualmente sólo contamos con estudios solventes sobre aquellos cuya gestión reformadora de la Marina destacó sobre la de los demás. Julián de Arriaga fue uno de los secretarios de Marina de los que apenas conocíamos unos rasgos. Este trabajo nos acerca al tiombre que fue y a su familia, y da a conocer su carrera como marino de guerra, desarrollada durante la primera mitad del siglo, antes de acceder a la cartera de Marina. One of the reforms that king Philip V did to modernice the Spanish administration was to create a ministry for the Navy. During the 18th century this ministry had sixteen different ministers. For the moment we know only the biography of the most outstanding ministers, those who did fundamental reforms for the Spanish Navy. Julián de Arriaga is one of the ministers of the Navy of whom we know only some few characteristics. This article deals with his family and his career as a naval oficer before his promotion to the ministry in 1754.

  16. The role of marinas and recreational boating in the occurrence and distribution of exotic caprellids (Crustacea: Amphipoda) in the Western Mediterranean: Mallorca Island as a case study

    Science.gov (United States)

    Ros, Macarena; Vázquez-Luis, Maite; Guerra-García, José M.

    2013-10-01

    In the Mediterranean Sea, the number of alien marine crustacean species has increased over the past two decades. However, knowledge about small alien marine crustaceans, like caprellid amphipods, is still very scarce. To understand the role of marinas and recreational boating in the early step of the invasion process by non-indigenous caprellids, we studied the recreational boating pressure and the spatial distribution of caprellid species in Mallorca Island. We collected caprellids from 14 marinas and 9 exposed intertidal rocky shores between November 2011 and April 2012 and we analyzed the differences in habitat use of native and exotic caprellids. Eight caprellid species, six native and two exotic, were found. Alien caprellids were only present in marinas, reaching high densities of population. The analysis of recreational boating pressure reveals that Palma-Migjorn is the area that is subject to the highest potential risk of introduction of exotic species via ship fouling. In the secondary dispersal of alien caprellids, the study reflects that recreational boating seems effective as a secondary vector in the transport of exotic species from marinas to marinas but not from marinas to natural and exposed areas. An illustrated key of caprellids from Balearic Island is provided to differentiate native and non-indigenous species.

  17. Rhabdias paraensis sp. nov.: a parasite of the lungs of Rhinella marina (Amphibia: Bufonidae) from Brazilian Amazonia.

    Science.gov (United States)

    Santos, Jeannie Nascimento dos; Melo, Francisco Tiago de Vasconcelos; Nascimento, Luciana de Cássia Silva do; Nascimento, Daisy Esther Batista do; Giese, Elane Guerreiro; Furtado, Adriano Penha

    2011-06-01

    The nematode parasites of Rhinella marina include species of the genus Rhabdias (Rhabdiasidae: Rhabditoidea). The present study describes Rhabdias paraensis sp. nov., which parasitizes the lungs of R. marina in Brazilian Amazonia. Of the more than 70 known species of this genus, 18 are parasites of bufonids, of which, eight are Neotropical. The new species described here is similar to Rhabdias alabialis in the absence of lips is different by the presence of conspicuous cephalic papillae. We describe details of the four rows of pores, which are distributed equally along the whole of the length of the body and connected with hypodermal cells, using histology and scanning electron microscopy. Other histological aspects of the internal structure of this nematode are also described.

  18. [Marina de Vasconcellos and the social sciences in Rio de Janeiro: a study of the social circles].

    Science.gov (United States)

    Ribeiro, Adelia Maria Miglievich

    2008-06-01

    An investigation of the career of one of the "founding mothers" of the social sciences in Rio de Janeiro, Marina de Vasconcellos, successor of Arthur Ramos, is one way of understanding how anthropology was established in Rio de Janeiro. Conflicts and alliances, continuities and discontinuities, lie behind the pioneering Brazilian Society of Anthropology and Ethnology and the Institute of Social Sciences, both at Faculdade Nacional de Filosofia. Marina de Vasconcellos' professional life bore the marks of the clash between different schools of thought regarding anthropology at a time when university courses were being introduced. As a professor, she was committed to educating new professionals, and in 1968, she was steadfast in the struggle for university autonomy. The study leads to a reflection upon the criteria for success in academia, countering the view that this depends entirely on the publication of books and articles.

  19. Morphological characterization of Eustrongylides sp. larvae (Nematoda, Dioctophymatoidea parasite of Rhinella marina (Amphibia: Bufonidae from Eastern Amazonia

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    Francisco Tiago de Vasconcelos Melo

    2016-01-01

    Full Text Available Absctract Eustrongylides spp. nematodes have birds as final hosts and uses other vertebrates as intermediate/paratenic host (fish, amphibians and reptiles and have zoonotic potential. In amphibians, the larvae may be located in the subcutaneous tissues, liver and mesentery, between the muscle fibres, especially in the lower limbs. Rhinella marina, which is widely observed in Brazil, has exhibited complex diversity in its helminth fauna, reflecting the unique habitat of the Amazon biome. For the first time, this study describes the morphological aspects of third-stage larvae of Eustrongylides sp. in Rhinella marina from Santa Cruz do Ararí, Marajó Archipelago, Eastern Amazonia, using light and scanning electron microscopy.

  20. Assessment of trace metal bioaccumulation by Avicennia marina (Forsk.) in the last remaining mangrove stands in Manila Bay, the Philippines.

    Science.gov (United States)

    Gabriel, Ana Veronica S; Salmo, Severino G

    2014-12-01

    Concentrations of lead (Pb), copper (Cu), zinc (Zn), and cadmium (Cd) were evaluated in the sediments, roots and leaves of a mangrove species (Avicennia marina) in Las Piñas-Parañaque Critical Habitat and Ecotourism Area (LPPCHEA), Manila Bay. The concentrations showed a general pattern of Zn > Pb > Cu > Cd in sediments, Cu > Pb > Zn > Cd in roots and Cu > Zn > Pb > Cd in leaves. The trace metal concentrations in both sediments and plant tissues were below contamination threshold levels. Based on computed bioaccumulation indices, A. marina could be used for the phytostabilization and phytoextraction of Cu and Cd. The LPPCHEA mangrove ecosystem is an ecologically important ecosystem that will limit the spread of trace metals to the surrounding environment.

  1. EVALUACIÓN CITOTÓXICA DE FRACCIONES OBTENIDAS DE LA ESPONJA MARINA DEL CARIBE COLOMBIANO Topsentia ophiraphidites

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    LINA BLANDÓN

    2013-01-01

    Full Text Available A partir de las esponjas marinas se han obtenido gran cantidad de sustancias con potencial antitumoral. En este estudio se realizó la evaluación de la actividad citotóxica, de cinco  fracciones de la esponja marina del Caribe colombiano Topsentia ophiraphidites en las líneas celulares Jurkat y CHO, mediante las pruebas de MTT y azul de tripano. Sólo la fracción número 4 (T4 presentó actividad citotóxica significativa, obteniendo una concentración inhibitoria media (IC50 de 33 µg/mL para las células Jurkat y 58 µg/mL para las células CHO, sugiriendo la necesidad de realizar posteriores ensayos de genotoxicidad y efectos sobre el ciclo celular.

  2. Susceptibility of seagrass to oil spills: A case study with eelgrass, Zostera marina in San Francisco Bay, USA.

    Science.gov (United States)

    Fonseca, Mark; Piniak, Gregory A; Cosentino-Manning, Natalie

    2017-02-15

    Existing literature illustrates inconsistent responses of seagrasses to oil exposure, both in the field and in the laboratory. Here, we add a new study that combined morphometric, demographic and photophysiology assessments to determine the potential oiling impacts to eelgrass (Zostera marina) from the 2007 Cosco Busan event in San Francisco Bay. Shoot densities, reproductive status, and rhizome elongation of Z. marina were examined at sites with pre-spill data, and eelgrass photosynthetic efficiency was measured post-spill. Shoot densities and percent elongation of rhizome internodes formed after the oil spill varied but with no consistent relationship to adjacent shoreline cleanup assessment team (SCAT) oiling categories. Similarly, differences in seagrass photosynthetic efficiency were not consistent with SCAT oiling categories. While thresholds for negative impacts on seagrass in general remain to be defined, conclusive oiling indicators for degree and duration of exposure would be important considerations and need examination under controlled study. Copyright © 2016 Elsevier Ltd. All rights reserved.

  3. High-quality-draft genome sequence of the yellow-pigmented flavobacterium Joostella marina type strain (En5(T)).

    Science.gov (United States)

    Stackebrandt, Erko; Chertkov, Olga; Lapidus, Alla; Nolan, Matt; Lucas, Susan; Han, Cliff; Cheng, Jan-Fang; Tapia, Roxanne; Goodwin, Lynne A; Bruce, David; Pitluck, Sam; Liolios, Konstantinos; Mavromatis, Konstantinos; Pagani, Ioanna; Ivanova, Natalia; Mikhailova, Natalia; Huntemann, Marcel; Pati, Amrita; Chen, Amy; Palaniappan, Krishna; Rohde, Manfred; Tindall, Brian J; Göker, Markus; Woyke, Tanja; Detter, John C; Bristow, James; Eisen, Jonathan A; Markowitz, Victor; Hugenholtz, Philip; Klenk, Hans-Peter; Kyrpides, Nikos C

    2013-04-15

    At present, Joostella marina Quan et al. 2008 is the sole species with a validly published name in the genus Joostella, family Flavobacteriacae, phylum Bacteriodetes. It is a yellow-pigmented, aerobic, marine organism about which little has been reported other than the chemotaxonomic features required for initial taxonomic description. The genome of J. marina strain En5(T) complements a list of 16 Flavobacteriaceae strains for which complete genomes and draft genomes are currently available. Here we describe the features of this bacterium, together with the complete genome sequence, and annotation. This is the first member of the genus Joostella for which a complete genome sequence becomes available. The 4,508,243 bp long single replicon genome with its 3,944 protein-coding and 60 RNA genes is part of the G enomic E ncyclopedia of Bacteria and Archaea project.

  4. Análisis preliminar de la estructura primaria y secundaria del ARNtTrp en tortugas marinas

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    Harvey Infante-Rojas

    2015-06-01

    Full Text Available Actualmente existen siete especies de tortugas marinas, todas amenazadas o en riesgo inminente de extinción. Los estudios con ADN mitocondrial han permitido hacer acercamientos sobre filogenia, evolución, rutas migratorias y centros de dispersión, además para la identificación de polimorfismos y haplotipos, siendo base para planes de manejo y conservación. El presente estudio representa la primera descripción comparada de la estructura primaria y secundaria del ARNtTrp mitocondrial en tortugas marinas. Se realizó un alineamiento múltiple de 26 secuencias del gen que codifica para el ARNtTrp y se propuso la estructura secundaria utilizando el programa ARWEN. Se identificaron potenciales interacciones terciarias por homología comparada con el ARNtTrp de mamíferos. Los resultados mostraron una secuencia consenso de 76 bases con siete regiones conservadas que representan el 76 % de la molécula. Se identificaron polimorfismos que representan tres haplotipos para C. caretta, dos para C. mydas y uno para cada una de las demás especies. Las estructuras secundarias mostraron cambios nucleotídicos puntuales para cada especie y también mostraron que el tallo aceptor, el brazo TψC y el bucle anticodón son motivos conservados en el ARNtTrp de las tortugas marinas. Se encontró un enlace no canónico tipo A-A en el tallo DHU que podría considerarse característico de tortugas marinas. Además, se obtuvo una estructura secundaria consenso en donde se identificaron las siete regiones conservadas, seis posibles interacciones terciarias y el bucle DHU como región variable.

  5. Growth responses of the mangrove Avicennia marina to salinity: development and function of shoot hydraulic systems require saline conditions.

    Science.gov (United States)

    Nguyen, Hoa T; Stanton, Daniel E; Schmitz, Nele; Farquhar, Graham D; Ball, Marilyn C

    2015-02-01

    Halophytic eudicots are characterized by enhanced growth under saline conditions. This study combines physiological and anatomical analyses to identify processes underlying growth responses of the mangrove Avicennia marina to salinities ranging from fresh- to seawater conditions. Following pre-exhaustion of cotyledonary reserves under optimal conditions (i.e. 50% seawater), seedlings of A. marina were grown hydroponically in dilutions of seawater amended with nutrients. Whole-plant growth characteristics were analysed in relation to dry mass accumulation and its allocation to different plant parts. Gas exchange characteristics and stable carbon isotopic composition of leaves were measured to evaluate water use in relation to carbon gain. Stem and leaf hydraulic anatomy were measured in relation to plant water use and growth. Avicennia marina seedlings failed to grow in 0-5% seawater, whereas maximal growth occurred in 50-75% seawater. Relative growth rates were affected by changes in leaf area ratio (LAR) and net assimilation rate (NAR) along the salinity gradient, with NAR generally being more important. Gas exchange characteristics followed the same trends as plant growth, with assimilation rates and stomatal conductance being greatest in leaves grown in 50-75% seawater. However, water use efficiency was maintained nearly constant across all salinities, consistent with carbon isotopic signatures. Anatomical studies revealed variation in rates of development and composition of hydraulic tissues that were consistent with salinity-dependent patterns in water use and growth, including a structural explanation for low stomatal conductance and growth under low salinity. The results identified stem and leaf transport systems as central to understanding the integrated growth responses to variation in salinity from fresh- to seawater conditions. Avicennia marina was revealed as an obligate halophyte, requiring saline conditions for development of the transport systems

  6. Annual variation of biomass and photosynthesis in Zostera marina L. along the Pacific Coast of Baja California, Mexico

    Science.gov (United States)

    Cabello-Pasini, Alejandro; Munoz-Salazar, R.; Ward, D.H.

    2003-01-01

    Density, biomass, morphology, phenology and photosynthetic characteristics of Zostera marina were related to continuous measurements of in situ irradiance, attenuation coefficient and temperature at three coastal lagoons in Baja California, Mexico. In situ irradiance was approximately two-fold lower at San Quintin Bay (SQ) than at Ojo de Liebre Lagoon (OL) and San Ignacio Lagoon (SI). As a consequence of the greater irradiance plants at OL and SI were established 1 m deeper within the water column than those at SQ. At SQ, there was a four-fold variation in biomass of Z. marina caused by changes on shoot length and not shoot density, while at OL and SI biomass and shoot length did not fluctuate significantly throughout the year. Reproductive shoot density reached maximum values concomitantly with the greater irradiance during spring-summer, however, the density was approximately three-fold greater at SQ than at the southern coastal lagoons. While irradiance levels were two-fold greater at the southern lagoons, in general, photosynthetic characteristics were similar among all three lagoons. The hours of light saturated photosynthesis, calculated from their photosynthetic characteristics and irradiance measurements, suggest that photosynthesis of shoots from OL and SI are saturated for more than 6 h per day throughout the year, while shoots from SQ are likely light limited during approximately 15% of the year. Consequently, an increase in attenuation coefficient values in the water column will likely decrease light availability to Z. marina plants at SQ, potentially decreasing their survival.

  7. Effects of conventional and biodegradable microplastics on a marine ecosystem engineer (Arenicola marina) and sediment nutrient cycling.

    Science.gov (United States)

    Green, Dannielle Senga; Boots, Bas; Sigwart, Julia; Jiang, Shan; Rocha, Carlos

    2016-01-01

    Effects of microplastic pollution on benthic organisms and ecosystem services provided by sedimentary habitats are largely unknown. An outdoor mesocosm experiment was done to realistically assess the effects of three different types of microplastic pollution (one biodegradable type; polylactic acid and two conventional types; polyethylene and polyvinylchloride) at increasing concentrations (0.02, 0.2 and 2% of wet sediment weight) on the health and biological activity of lugworms, Arenicola marina (Linnaeus, 1758), and on nitrogen cycling and primary productivity of the sediment they inhabit. After 31 days, A. marina produced less casts in sediments containing microplastics. Metabolic rates of A. marina increased, while microalgal biomass decreased at high concentrations, compared to sediments with low concentrations or without microplastics. Responses were strongest to polyvinylchloride, emphasising that different materials may have differential effects. Each material needs to be carefully evaluated in order to assess their risks as microplastic pollution. Overall, both conventional and biodegradable microplastics in sandy sediments can affect the health and behaviour of lugworms and directly or indirectly reduce primary productivity of these habitats. Copyright © 2015 Elsevier Ltd. All rights reserved.

  8. Anonymous nuclear loci in the white-faced storm-petrel Pelagodroma marina and their applicability to other Procellariiform seabirds.

    Science.gov (United States)

    Silva, Mónica C; Duarte, Margarida A; Coelho, M Manuela

    2011-01-01

    Procellariiform seabirds are among the avian species with the fastest rates of extinction due to interactions with fisheries and introduction of alien predators to the breeding colonies. Conservation and management policies targeting populations of these species must include information on colony demographics and levels of isolation and genetic markers go a long way toward providing reliable estimates of these parameters. To this end, we report isolation and characterization of 14 anonymous nuclear loci, with average length of 657 bp, in the pelagic seabird White-faced Storm-petrel Pelagodroma marina, a species for which there is virtually no genetic information available. These loci, initially isolated from a genomic library built from P. marina, were further tested, for a range of conditions, in 7 other species representing all Procellariiform families. We found high levels of cross-species amplification success, varying between 79% and 86% in representatives of Diomedeidae, Procellariidae, Pelecanoididae, and other Hydrobatidae. We also sequenced 11 loci for 22 P. marina individuals and report higher levels of anonymous genetic variation (π = 0.002), with an average of 1 single nucleotide polymorphism every 100 bp surveyed, relative to the levels found on a typically variable intron in avian species. These markers will be a valuable tool in future population genetics and phylogenetic studies, particularly of nonmodel seabird species.

  9. Trace elements in the sediments of a large Mediterranean marina (Port Camargue, France): levels and contamination history.

    Science.gov (United States)

    Briant, Nicolas; Bancon-Montigny, Chrystelle; Elbaz-Poulichet, Françoise; Freydier, Rémi; Delpoux, Sophie; Cossa, Daniel

    2013-08-15

    The study of trace elements (Cu, Zn, Pb, As, Hg) and butyltin concentrations in the sediments of Port Camargue enabled assessment of the levels and history of the contamination of the largest European marina linked with the use of antifouling paints. Surface sediments near the boat maintenance area were heavily contaminated with up to 1497 μg g(-1) of Cu, 475 μg g(-1) of Zn, 0.82 μg g(-1) of Hg, 94 μg g(-1) of Pb and over 10,000 ngSn g(-1) of tributyltin (TBT). High concentrations of Hg and TBT indicate ongoing sources of these elements despite the ban on their use as biocides in paints. Sediment cores provided records of contamination since 1969. The peak concentrations of As, Hg, Pb and TBT in the sediment profile reflect their presence on boat hulls when the marina was built at the end of the 1960s. Degradation of TBT in the sediments near the boat maintenance area is slow compared to other less contaminated area of the marina. Copyright © 2013 Elsevier Ltd. All rights reserved.

  10. Bioaccumulation and distribution of metals in sediments and Avicenna marina tissues in the Hara Biosphere Reserve, Iran.

    Science.gov (United States)

    Nowrouzi, Mohsen; Pourkhabbaz, Alireza; Rezaei, Mohammadreza

    2012-10-01

    The metal pollution in Sediments and Avicenna marina tissues in the Hara Biosphere Reserve was monitored for Lead (Pb), Cadmium (Cd), and Nickel (Ni) with atomic absorption spectrometer. The results showed that the mean concentration of Pb, Cd, and Ni in the water and sediments were much higher than the recommended threshold limits in the most stations, also the highest means of Pb, Cd, and Ni were observed in Avicenna roots and it were 25.26 ± 4.86, 2.17 ± 0.74, and 26.72 ± 6.17 (μg g(-1)) respectively. Calculating BCF (bioconcentration factor) index illustrates that A. marina accumulates Pb, Cd, and Ni 1.62, 1.52 and 0.73 times greater than sediment levels respectively, So it can show that A. marina may be employed as a biological indicator exposure of Cd, Pb, and Ni with temporal monitoring, also the factories were main sources of metals contamination in the Hara Biosphere Reserve.

  11. Texture and composition of the Rosa Marina beach sands (Adriatic coast, southern Italy: a sedimentological/ecological approach

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    Moretti Massimo

    2016-06-01

    Full Text Available Beach sands from the Rosa Marina locality (Adriatic coast, southern Italy were analysed mainly microscopically in order to trace the source areas of their lithoclastic and bioclastic components. The main cropping out sedimentary units were also studied with the objective to identify the potential source areas of lithoclasts. This allowed to establish how the various rock units contribute to the formation of beach sands. The analysis of the bioclastic components allows to estimate the actual role of organisms regarding the supply of this material to the beach. Identification of taxa that are present in the beach sands as shell fragments or other remains was carried out at the genus or family level. Ecological investigation of the same beach and the recognition of sub-environments (mainly distinguished on the basis of the nature of the substrate and of the water depth was the key topic that allowed to establish the actual source areas of bioclasts in the Rosa Marina beach sands. The sedimentological analysis (including a physical study of the beach and the calculation of some statistical parameters concerning the grain-size curves shows that the Rosa Marina beach is nowadays subject to erosion.

  12. Direct and indirect effects of the herbicides Glyphosate, Bentazone and MCPA on eelgrass (Zostera marina).

    Science.gov (United States)

    Nielsen, Line Winkel; Dahllöf, Ingela

    2007-04-20

    Eelgrass beds are important habitats for many organisms, but there has been a decline in the area covered by eelgrass during the last decades due to increased eutrophication resulting in increased shading from phytoplankton. The use of herbicides in terrestrial agriculture has also increased over the last century, and while the effects of herbicides on non-target organisms have been well studied in freshwater they are overlooked in coastal waters. It is not known if herbicides have any effect on the distribution of eelgrass (Zostera marina), or how natural phytoplankton communities respond to the same herbicides. Direct and indirect effects of the herbicides Glyphosate, Bentazone and MCPA both as single toxicants and as mixtures, on the eelgrass plants were investigated in this study. The direct effects on eelgrass were examined by measuring the four different endpoints; the relatively growth rate as length and weight, the chlorophyll a and b ratio, as well as the RNA-DNA ratio, at the end of a 3 days exposure period. The indirect effect was investigated by measuring the effect on a natural phytoplankton production from Roskilde Fjord six times during 1 year. The results showed that the chlorophyll a-b and RNA-DNA ratios were the most sensitive endpoints in single herbicide experiments. The effects of herbicide mixtures on eelgrass were much larger compared to the single substances. Nearly a halving was found for both the relatively growth rate in length and weight, and the RNA-DNA as well as the chlorophyll a-b ratios were also significant reduced. This indicates a possible synergistic effect, and calculations based on the concentration addition model indicate that the low concentrations mixture has a synergistic effect, whereas the high concentration mixture has an antagonistic effect on eelgrass (Z. marina). The low concentrations mixture is the one with the highest relevance for coastal areas. The effect on phytoplankton showed some variation over the year but

  13. The effect of aqueous extract of Avicennia marina (Forsk. Vierh. leaves on liver enzymes' activity, oxidative stress parameters and liver histopathology in male diabetic rats

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    Akram Hamzevi

    2017-12-01

    Full Text Available Background: Avicennia marina has antioxidant and anti-diabetic properties. This study was conducted to examine the effect of aqueous extract of A. marina on liver enzymes' activity, oxidative stress parameters and liver histopathology in diabetic rats. Materials and Methods: In this experimental study, 28 male rats were allocated into the equal groups of control, diabetic control and experimental diabetic 1 and 2. The diabetes in diabetic control and experimental diabetic groups was induced using an intraperitoneal injection of 120 mg/kg alloxan. The experimental diabetic groups received the aqueous extract of A. marina (100 and 200 mg/kg, i.p. in alternate days for one month. Sterile distilled water was injected to the animals of control and diabetic control groups. At the end of the treatment period, serum levels of ALT, AST, GGT and ALP were measured. Then, levels of SOD, GST, CAT and MDA were measured in the liver tissue. The liver sections were prepared and examined by an optical microscope. Results: Results showed that administration of the A. marina extract (100 and 300 mg/kg, ip to the diabetic rats significantly decreased the serum levels of liver enzymes and tissue level of MDA. Also, the activity of the liver tissue's antioxidant enzymes was increased (P<0.05. The A. marina extract dose-dependently decreased liver damages in diabetic rats. Conclusion: Administration of the A. marina extract improves liver tissue oxidative stress indices and decreases the serum level of liver enzymes. Also, A. marina extract improves liver tissue injuries induced by diabetes.

  14. Disturbance of benthic infauna by sediment-reworking activities of the lugworm Arenicola marina

    Science.gov (United States)

    Flach, E. C.

    The influence of the lugworm Arenicola marina on the abundance of other benthic species was studied in the westernmost part of the Wadden Sea. Small squares (1 m 2) within depopulated 144-m 2 plots were recolonized with various (naturally-occurring) lugworm densities (0-10-20-40-80 and 0-25-50-75-100 per m 2). These plots were sampled during the summer. Lugworms were found to have a strongly negative effect on the densities of C. volutator. At 0-density lugworms, the numbers of C. volutator were high. These were halved at 17 lugworms per m 2 ( i.e. the mean density on the tidal flats of the Dutch Wadden Sea), and were further reduced at higher lugworm densities ( e.g. 20% remained at 40 lugworms per m 2). Laboratory observations of Corophium behaviour in the presence of Arenicola suggest that sediment-reworking lugworms stimulate Corophium to emigrate. Effects of lugworms on other benthic species were also studied in the same way. Lugworms were found to have strongly negative effects on the juvenile densities of various worm and bivalve species ( Nereis diversicolor, Nephtys hombergii, Heteromastus filiformis, Scoloplos armiger, Pygospio elegans, Capitella capitata and Mya arenaria, Cerastoderma edule, Macoma balthica, Angulus tenuis, respectively).

  15. A new species of Mesocoelium (Digenea: Mesocoeliidae) found in Rhinella marina (Amphibia: Bufonidae) from Brazilian Amazonia.

    Science.gov (United States)

    Gomes, Tássia F F; Melo, Francisco T V; Giese, Elane G; Furtado, Adriano P; Gonçalves, Evonnildo C; Santos, Jeannie N

    2013-04-01

    Mesocoelium lanfrediae sp. nov. (Digenea: Mesocoeliidae) inhabits the small intestine of Rhinella marina (Amphibia: Bufonidae) and is described here, with illustrations provided by light, scanning electron microscopy and molecular approachs. M. lanfrediae sp. nov. presents the typical characteristics of the genus, but is morphometrically and morphologically different from the species described previously. The main diagnostic characteristics of M. lanfrediae sp. nov. are (i) seven pairs of regularly-distributed spherical papillae on the oral sucker, (ii) ventral sucker outlined by four pairs of papillae distributed in a uniform pattern and interspersed with numerous spines, which are larger at the posterior margin and (iii) small, rounded tegumentary papillae around the opening of the oral sucker, which are morphologically different from those of the oral sucker itself, some of which are randomly disposed in the ventrolateral tegumentary region of the anterior third of the body. Addionally, based on SSU rDNA, a phylogenetic analysis including Brachycoeliidae and Mesocoeliidae taxa available on GenBank established the close relationship between M. lanfrediae sp. nov. and Mesocoelium sp.

  16. A new species of nematode (Molineidae) from Rhinella marina (Amphibia: Bufonidae) in Guerrero, México.

    Science.gov (United States)

    Ruiz-Torres, Nallely; García-Prieto, Luis; Osorio-Sarabia, David; Violante-González, Juan

    2013-06-01

    Oswaldocruzia lamotheargumedoi n. sp., inhabiting the intestine of the cane toad, Rhinella marina (L.), in Laguna de Coyuca, Guerrero, México, is described here. The new species differs from 10 congeners infecting bufonid hosts because it has a type I bursa. In contrast, 7 of these species have type II bursa and 3 more a type III bursa. The species most similar to the species described herein is Oswaldocruzia pipiens Walton, 1929 . These 2 species share traits such as body size, bursa type, presence of cervical alae, and dorsal ray morphology. Nevertheless, both species can be distinguished based on the number of synlophe ridges at mid-body (54-56 for O. lamotheargumedoi vs. 45-48 for O. pipiens) and by the presence of a chitinous support in the long, and well developed, cervical alae of O. pipiens. In the new species, these structures are short, poorly developed, and lack chitinous support. Previous records of species of Oswaldocruzia in México include Oswaldocruzia subauricularis (Rudolphi, 1819) Travassos, 1917 in the Neotropical Realm and O. pipiens in the Nearctic.

  17. Petróleo, seguridad ambiental y exploración petrolera marina en Colombia

    Directory of Open Access Journals (Sweden)

    Avellaneda Cusaría, Alfonso

    2005-01-01

    Full Text Available Este artículo reseña un siglo de las actividades petroleras en Colombia, sus consecuencias en los territorios indígenas, así como los riesgos ambientales y para la salud, destacando por -otra parte- el papel que han asumido algunas compañías para controlar esos riesgos. Se discute las implicaciones sociales y ambientales, directas e indirectas, de las distintas fases de las actividades petroleras en la parte continental del país, y sus relaciones históricas con los procesos de violencia donde ha habido explotación petrolera desde el siglo XX. Finalmente, se abre una discusión sobre la nueva política petrolera del Estado colombiano, con la exploración costa fuera y la ausencia de control ambiental para proteger los ecosistemas que subyacen la licitación de áreas para los próximos años, sin asumir el derecho internacional en la protección marina.

  18. Restoring Resiliency: Case Studies from Pacific Northwest Estuarine Eelgrass (Zostera marina L.) Ecosystems

    Energy Technology Data Exchange (ETDEWEB)

    Thom, Ronald M.; Diefenderfer, Heida L.; Vavrinec, John; Borde, Amy B.

    2012-01-01

    The purpose of many ecological restoration projects is to establish an ecosystem with fully developed structure and function that exhibits resistance to and resilience from disturbances. Coastal restoration projects in the Pacific Northwest provide opportunities to understand what is required to restore the resilience of eelgrass (Zostera marina L.) populations. Factors influencing resilience observed in three case studies of eelgrass restoration include minimum viable population, adaptations of transplant populations, and natural and anthropogenic disturbances at restoration sites. The evaluation of resiliency depends on selecting appropriate monitoring metrics and the frequency and duration of monitoring. Eelgrass area, cover and shoot density provide useful and reliable metrics for quantifying resilience of restored meadows. Further, five years of monitoring of these metrics provides data that can reasonably predict the long-term viability of a planted plot. Eelgrass appears to be a resilient ecosystem in general, though one that data suggest may exhibit tipping points brought about by compounded environmental conditions outside of its tolerance ranges. Explicit inclusion of resilience in the planning and practice of habitat restoration may reduce uncertainties and improve the performance of restored systems by increasing buffering capacity, nurturing sources of renewal (e.g., seeds and rhizomes), and managing for habitat forming and maintaining processes (e.g., sediment dynamics) at multiple scales.

  19. Invader immunology: invasion history alters immune system function in cane toads (Rhinella marina) in tropical Australia.

    Science.gov (United States)

    Brown, Gregory P; Phillips, Benjamin L; Dubey, Sylvain; Shine, Richard

    2015-01-01

    Because an individual's investment into the immune system may modify its dispersal rate, immune function may evolve rapidly in an invader. We collected cane toads (Rhinella marina) from sites spanning their 75-year invasion history in Australia, bred them, and raised their progeny in standard conditions. Evolved shifts in immune function should manifest as differences in immune responses among the progeny of parents collected in different locations. Parental location did not affect the offspring's cell-mediated immune response or stress response, but blood from the offspring of invasion-front toads had more neutrophils, and was more effective at phagocytosis and killing bacteria. These latter measures of immune function are negatively correlated with rate of dispersal in free-ranging toads. Our results suggest that the invasion of tropical Australia by cane toads has resulted in rapid genetically based compensatory shifts in the aspects of immune responses that are most compromised by the rigours of long-distance dispersal. © 2014 John Wiley & Sons Ltd/CNRS.

  20. Monitoreo de tortugas marinas Lepidochelis olivaceae (paslama en playa La Flor - Rivas

    Directory of Open Access Journals (Sweden)

    Celia María Gutiérrez

    1999-12-01

    Full Text Available La tortuga marina Lepidochelis olivaceae desova en forma masiva, solamente en seis playas del mundo. Nicaragua cuenta con dos de estas playas en los sitios geográficos llamados ' “Chacocente", en el Departamento de Carazo y "La Flor", en San Juan del Sur. Esta especie de tortuga se caracteriza por presentar un movimiento sincronizado para su anidación en la playa, el cual se ha denominado arribadas. Durante 5 años consecutivos se realizó en el refugio de vida silvestre "La Flor", un monitoreo de la población anidante y se encontró que cada año durante el período de julio a enero, visitan esta playa aproximadamente, 3,000 a 12,000 tortugas, reportándose entre septiembre y octubre el pico más alto de la población anidante. Paralelo a este estudio, se realizó un diagnóstico rápido en las comunidades aledañas al refugio, el cual refleja que es una práctica tradicional de los pobladores, la extracción de huevos de tortuga para el consumo de la familia y para el comercio. Se considera, sin embargo, que esta práctica no representa económicamente una dependencia absoluta, ya que la población tiene otros tipos de actividades agropecuarias que contribuyen a su subsistencia.

  1. Giant toads (Rhinella marina) living in agricultural areas have altered spermatogenesis.

    Science.gov (United States)

    McCoy, Krista A; Amato, Ciro M; Guillette, Louis J; St Mary, Colette M

    2017-12-31

    Across diverse taxa, germ cell development is controlled by an intricate cascade of processes that are tightly controlled by the hypothalamic-pituitary-gonadal axis. Endocrine disturbances, such as those induced by endocrine disrupting chemicals (EDCs) can negatively affect spermatogenesis. Here, we investigate whether spermatogenesis is altered in the giant toad, Rhinella marina, living in agricultural areas where EDCs are used relative to suburban areas. We also ask if reductions in spermatogenesis were associated with developmental gonadal abnormalities (intersex) found in the same frogs. We found that toads in agricultural areas exhibited reduced spermatogenesis relative to non-agricultural animals, and that those reductions were not associated with gross gonadal abnormalities. All toads living in agricultural areas had reduced spermatogenesis relative to those living in non-agricultural areas regardless of whether they had gonadal abnormalities originating during development. Similarities in reproductive dysfunction among diverse taxa living in agricultural areas, including humans, suggest that many vertebrate taxa living in agricultural areas around the globe are likely experiencing some level of reproductive dysfunction. Copyright © 2017 Elsevier B.V. All rights reserved.

  2. A new species of Mesocoelium (Digenea: Mesocoeliidae) found in Rhinella marina (Amphibia: Bufonidae) from Brazilian Amazonia

    Science.gov (United States)

    Gomes, Tássia FF; Melo, Francisco TV; Giese, Elane G; Furtado, Adriano P; Gonçalves, Evonnildo C; Santos, Jeannie N

    2013-01-01

    Mesocoelium lanfrediaesp. nov. (Digenea: Mesocoeliidae) inhabits the small intestine of Rhinella marina (Amphibia: Bufonidae) and is described here, with illustrations provided by light, scanning electron microscopy and molecular approachs. M. lanfrediae sp. nov. presents the typical characteristics of the genus, but is morphometrically and morphologically different from the species described previously. The main diagnostic characteristics of M. lanfrediae sp. nov. are (i) seven pairs of regularly-distributed spherical papillae on the oral sucker, (ii) ventral sucker outlined by four pairs of papillae distributed in a uniform pattern and interspersed with numerous spines, which are larger at the posterior margin and (iii) small, rounded tegumentary papillae around the opening of the oral sucker, which are morphologically different from those of the oral sucker itself, some of which are randomly disposed in the ventrolateral tegumentary region of the anterior third of the body. Addionally, based on SSU rDNA, a phylogenetic analysis including Brachycoeliidae and Mesocoeliidae taxa available on GenBank established the close relationship between M. lanfrediae sp. nov. and Mesocoelium sp. PMID:23579798

  3. Ortleppascaris sp. and your host Rhinella marina: A proteomic view into a nematode–amphibian relationship

    Science.gov (United States)

    Silva, Jefferson Pereira e; Furtado, Adriano Penha; Santos, Jeannie Nascimento dos

    2014-01-01

    The success of the helminth–host relationship depends on a biochemical molecular arsenal. Perhaps the proteome is the largest and most important set of this weaponry, in which the proteins have a crucial role in vital processes to the parasite/host relationship, from basic metabolism and energy production to complex immune responses. Nowadays, the bioproducts expressed by the parasites are under the “spotlight” of immunoassays and biochemical analysis in helminthology, especially in proteomic analysis, which has provided valuable information about the physiology of the infecting agent. Looking into this point of view, why not turn to the infected agent as well? This study characterised the proteomic profile of fluid-filled fibrous cysts of encapsulated Ortleppascaris sp. larvae in the hepatic parenchyma of their intermediate host, the amphibian Rhinella marina. The proteins were separated by two-dimensional electrophoresis and identified by MS with the aid of Peptide Mass Fingerprint. A total of 54 molecules were analysed in this system, revealing a complex protein profile with molecules related to basic metabolic processes of the parasite, energy production, oxi-reduction and oxidative stress processes as well as molecules related to the host response. This study contributes to proteomic studies of protein markers of the development, infectivity, virulence and co-existence of helminths and their hosts. PMID:25161910

  4. Algas marinas bentónicas de la Isla Gorgona, costa pacífica colombiana

    Directory of Open Access Journals (Sweden)

    Mónica Murillo Muñoz

    2014-02-01

    Full Text Available Se presenta el listado de las algas marinas bénticas del Parque Nacional Natural Isla Gorgona, el inventario corresponde a la flora algal asociada a los diferentes ambientes marinos de la isla, a saber: arrecifes coralinos y fondos blandos. El monitoreo de las comunidades de algas se llevó a cabo entre octubre de 2010 y mayo de 2011, se realizó una estimación de abundancia y cobertura para lo cual se establecieron transeptos paralelos a la línea de costa y se evaluó el porcentaje de cobertura en cuadrantes de 0.25m². Se reportó un total de 43 especies para los dos ambientes estudiados (coralino y fondos blandos, 24 Rhodophyta, 12 Clorophyta, 6 Ochrophyta y una Cyanobacteria. El grupo más representativo fue el perteneciente a la clase Rhodophyceae, que correspondió al 55.8% de la flora inventariada. Los resultados de nuevos registros en la isla ratifican su importancia fitogeográfica, por cuanto la composición florística insular contribuye significativamente a la biodiversidad de la costa pacífica colombiana, destacándose la presencia de arrecifes coralinos como los principales ambientes marinos para el desarrollo de la flora algal de la región.

  5. Effects of salinity and water temperature on the ecological performance of Zostera marina

    DEFF Research Database (Denmark)

    Nejrup, Lars Brammer; Pedersen, Morten Foldager

    2008-01-01

    We tested the effects of salinity and water temperature on the ecological performance of eelgrass (Zostera marina L.) in culture-experiments to identify levels that could potentially limit survival and growth and, thus, the spatial distribution of eelgrass in temperate estuaries. The experiments...... included eight levels of salinity (2.5, 5, 10, 15, 20, 25, 30 and 35%) and seven water temperatures (5, 10, 15, 20, 25, 27.5 and 30 8C). Low salinity (i.e. 5 and 2.5%) increased mortality (3-6-fold) and had a strong negative effect on shoot morphology (number of leaves per shoot reduced by 40% and shoot...... biomass reduced by 30-40%), photosynthetic capacity (Pmax-reduced by 30-80%) and growth (production of new leaves reduced by 50-60%, leaf elongation rate reduced by 60-70% and production of side-shoots reduced by 40-60%), whereas eelgrass performed almost equally well at salinities between 10 and 35...

  6. Proteasas extracelulares producidas por bacterias marinas aisladas de aguas contaminadas con efluentes pesqueros

    Directory of Open Access Journals (Sweden)

    Tito Sánchez

    2013-06-01

    Full Text Available Un total de 26 cepas de bacterias marinas con actividad proteolítica fueron aisladas de agua de mar contaminadas con efluentes pesqueros; las mismas que se evaluaron en base al crecimiento y formación de halos de actividad en Agar Marino suplementados con caseína al 1%, pH 8,0 e incubados a 25 ºC por 72 h. Cinco cepas, seleccionadas por presentar los mejores halos de actividad fueron evaluadas a su vez por su crecimiento y producción de proteasas a diferentes concentraciones de NaCl, rangos de temperatura y pH; siendo consideradas finalmente como bacterias halotolerantes, psicrotróficas y alcalófilas moderadas. Estas cepas también fueron evaluadas por su actividad proteolítica específica sobre la caseína, siendo la cepa CM48 (Pseudomonas sp. la que presentó la mejor actividad específica (17,38 U/mg a las 72 horas, y seguidas por las cepas CM45 (Alcaligenes sp. (12,09 U/mg y tres cepas de Aeromonas sp. (CM43, CM44 y CM46 con valores de 12,02; 10,07 y 10,10 U/mg respectivamente.

  7. An ultrastructural study of spermatogenesis and sperm morula breakdown in Arenicola marina (L.) (Annelida: Polychaeta)

    Science.gov (United States)

    Pacey, A. A.; Bentley, M. G.

    1992-06-01

    Spermatogenesis in the lugworm Arenicola marina, in common with other members of Arenicolidae, occurs in the coelomic fluid and results in the formation of discs of mature spermatozoa known as a morulae. Within a morula, individual spermatozoa are connected by a common mass of cytoplasm called the cytophore and therefore make up a syncitium. Immediately prior to spawning, and in response to an endocrine substance known as “Sperm Maturation Factor” (SMF), the structure of the sperm morulae breaks down and free spermatozoa are liberated. These are subsequently spawned from the body cavity. The investigation described here uses transmission electron microscopy to investigate the ultrastructural changes, which accompany spermatogenesis and the breakdown of sperm morulae in response to SMF in vitro. The study demonstrates that the cytophore appears to have a key role both during spermatogenesis and during sperm morula breakdown. The ultrastructure of sperm morulae and of mature spermatozoa is described. The structure of spermatozoa is shown to be primitive with a single flagellum which appears to be coiled at its distal end. The phagocytosis of free spermatozoa by coelomocytes is also described and it is suggested that these may play a role in the resorption of unspawned gametes in vivo.

  8. Temporal and spatial dynamics of ephemeral drift-algae in eelgrass, Zostera marina, beds

    Science.gov (United States)

    Rasmussen, Jonas Ribergaard; Pedersen, Morten Foldager; Olesen, Birgit; Nielsen, Søren Laurentius; Pedersen, Troels Møller

    2013-03-01

    Aggregations of unattached, filamentous macroalgae showed high temporal and spatial dynamics in two shallow and relatively sheltered eelgrass (Zostera marina) beds in Aarhus Bay and the Isefjord, Denmark. The changes in algal abundance were followed in permanent plots at 1-3 days intervals during three different periods of the growth season (May-September). Drift-algal assemblages were present within the 3000 m2 study areas in relatively high and constant abundance (>47% cover) throughout the study period. However, significant changes in average site cover did occur on short timescales (days) suggesting that variability in algal cover may be undetected in monthly assessments. The changes in cover were caused either by algal growth or by physical forces moving large aggregations of algae into or out of the study area. Within plots (1 m2) variability was even higher and algal cover changed regularly between observations (days). Hence, the algae were continuously rearranged within the eelgrass beds; also during periods with no change in average algal cover. The variability in cover of individual plots was negatively correlated to eelgrass cover, suggesting that algae were retained by the eelgrass leaves. This highly dynamic nature of filamentous macroalgal aggregations in eelgrass beds should be considered when evaluating implications of macroalgal blooms for seagrass growth and survival. A frequent relocation of drift-algae at small spatial scale may moderate the formation of poor oxygen conditions within mats and shorten the duration of exposure experienced by individual shoots.

  9. Potential sources of bacteriological pollution for two bays with marinas in Trinidad

    Directory of Open Access Journals (Sweden)

    Christine Ann Bullock

    2005-05-01

    Full Text Available Welcome Bay and Chaguaramas Bay in the northwest peninsula of Trinidad contain large marinas and smaller sections of bathing beaches.Bacteriological surveys were conducted at both bays to assess water quality and to determine potential sources of pollution.These surveys were conducted during the wet season of 1996 and the dry season of 1997.Eleven sample stations were established at Welcome Bay and 12 at Chaguaramas Bay.Freshwater samples were collected from rivers and drains within the survey area.Marine water samples were collected from marinas,bathing beaches and inshore and outer areas at both bays.Five water samples were collected from each sampling station during the wet season of 1996 and six during the dry season of 1997.The membrane filter technique was used to determine faecal coliform and Escherichia coli levels in all samples. There was a seasonal effect on water quality,with significantly higher faecal coliform levels in the wet season, when water quality was not in compliance with international standards.This represents a potential health risk in bathing areas.Water quality was better at the outer area of both bays.Water quality at the inner bay areas was most likely adversely affected by land-based sources of pollution identified in this study.These sources include three drains and two rivers,which discharged into the bays.Yachts were apparently not a source of sewage pollution:there was no significant relationship between yacht number and faecal coliform levels.Las bahías Welcome y Chaguaramas en la península noroeste de Trinidad tienen grandes marinas y secciones pequeñas de playas para bañistas.Se realizaron sondeos bacteriológicos en ambas bahías para determinar la calidad del agua y para señalar fuentes potenciales de contaminación.Estos sondeos fueron realizados durante la época lluviosa de 1996 y la seca de 1997.Once estaciones de muestreo se establecieron en Bahía Welcome y doce en Bahía Chaguaramas.Muestras de

  10. Com'è profondo il mare Gli uominiche lo misurano, l'Istituto Idrografico della Marina

    Directory of Open Access Journals (Sweden)

    Alessandro Nobili

    2014-03-01

    Full Text Available Measuring sea depths: the Italian hydrographic InstituteSince 1873 the Italian Hydrographic Institute (Istituto Idrografico della Marina - IIM has been involved in sea depth measuring in order to improve safety of navigation. The main mission of the institute is to provide hydrographic services for waters of Italian national responsibility required by the International Convention for the Safety of Life at Sea (SOLAS. This role requires the coordination and determination of policy and standards which covers both hydrographic surveying and charting, as well as contributing tothe coordination, exchange and standards related to geospatial data in general. IIM’s traditions and methods take their origins from the pioneers of hydrography; in this paper the author briefl y describes the main stages in IIM’s history, passing throught scientifi c and international activities and concludine with challenges and prospects of digital era.

  11. Actividad antibacteriana y antifúngica de extractos de algas marinas venezolanas

    Directory of Open Access Journals (Sweden)

    Nurby Ríos

    2011-07-01

    Full Text Available En este trabajo se evaluaron las propiedades bioactivas antibacterianas y antimicóticas de 33 extractos (etanol, diclorometano, hexano obtenidos de 11 especies de algas marinas recolectadas en las localidades de San Juan de Los Cayos y Chichiriviche, Estado Falcón, Venezuela. La actividad antibiótica y antimicótica de los extractos se evaluó mediante la aparición de halos de inhibición contra bacterias Gram positivas (Staphylococ- cus aureus, Gram negativas (Pseudomona aeruginosa, Klebsiella pneumoniae, Escherichia coli y el hongo Candida albicans. De los 33 extractos ensayados sólo 17 presentaron actividad antibacteriana (5 con etanol, 6 con diclorometano y 6 con hexano, resultando activos 14 frente a las especies Gram(- y 4 contra la especie Gram(+. Las especies algales que mostraron actividad antibacteriana fueron: Acanthophora sp., Bryothamnion triquetrum, Gracilaria sp., Gelidium sp., Caulerpa mexicana, Caulerpa sp., Caulerpa spp., Halimeda incras- sata, Ulva sp., Codium decorticatum, Sargassum sp. Ninguno de los extractos de algas ensayados presentó actividad antimicótica sobre Cándida albicans. Los resultados obtenidos permiten concluir que las algas de la costa occidental de Venezuela, presentan compuestos bioactivos con actividad antibacteriana.

  12. Composición Esterólica de la Esponja Marina Xestospongia Muta

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    Carmenza Duque

    2009-10-01

    Full Text Available De la esponja marina Xestospongia muta recolectada en la bahía de Santa Marta, Caribe Colombiano, se aislaron por Cromatografía en Columna (CC sobre Silica gel, eluyendo con Benceno: Acetato de Etilo 10:2, dos fracciones esteróiicas; XMl (Rf = 0,28,Si02/Benceno; AcOEt 10:2 y XM2 (Rf = 0,24,SiO2/Benceno : AcOEt 10:2, Los esteróles presentes en estas dos fracciones fueron sometidos a Cromatografía Liquida de Alta Eficiencia (CLAE preparativa y analizados por Cromatografía de Gases (CG y Cromatografía de Gases acoplada en Espectometrla de Masas (CG-EM, Catorce esteróles fueron separados y 13 idenficados. La mayoría de ellos fueron 3n-0H-A^-Esteróles (Aproximadamente el 87% de los esteróles totales incluyendo al (24R + 24S-5,28-Estigmastadien-3i3-24-diol, mezclaepimérica por primera vez asi reportada en la naturaleza. El porcentaje restante correspondió a Estañóles y a un 6,2% de compuestos sin identificar.

  13. Caracterización parcial y espectro antimicrobiano de substancias inhibitorias producidas por Alteromonas marinas

    Directory of Open Access Journals (Sweden)

    Jorge León

    2014-06-01

    Full Text Available Se caracterizan parcialmente substancias inhibitorias producidas por cepas marinas del género Alteromonas spp. Pruebas de precipitación de extractos crudos de sobrenadantes de las cepas N22.C y N11.6 con concentraciones saturantes de sulfato de amonio y filtrados en columnas de Sephadex G-25 muestran la producción de substancias inhibitorias (SI de naturaleza proteinácea. Otros ensayos de caracterización revelan que dichas substancias tienen un amplio espectro de actividad antibiótica frente a cepas de colección patógenas de peces, moluscos y crustáceos. Asimismo, muestran a las SI como termosensibles a 90oC en 45 min y con capacidad de mantener estabilidad inhibitoria dentro, del rango de pH de 3,0 a 9,0 hasta por 15 min. La actividad antibiótica de las substancias frente a Vibrio anguillarum NCMB 2133, V. anguillarum ATCC 19264 y Aeromonas salmonicida 67,79 fueron comparativamente equivalentes a 30 ppm del antibiótico oxitetraciclina (OTC.

  14. Offshore Energy Mapping for Northeast Atlantic and Mediterranean: MARINA PLATFORM project

    Science.gov (United States)

    Kallos, G.; Galanis, G.; Spyrou, C.; Kalogeri, C.; Adam, A.; Athanasiadis, P.

    2012-04-01

    Deep offshore ocean energy mapping requires detailed modeling of the wind, wave, tidal and ocean circulation estimations. It requires also detailed mapping of the associated extremes. An important issue in such work is the co-generation of energy (generation of wind, wave, tides, currents) in order to design platforms on an efficient way. For example wind and wave fields exhibit significant phase differences and therefore the produced energy from both sources together requires special analysis. The other two sources namely tides and currents have different temporal scales from the previous two. Another important issue is related to the estimation of the environmental frequencies in order to avoid structural problems. These are issues studied at the framework of the FP7 project MARINA PLATFORM. The main objective of the project is to develop deep water structures that can exploit the energy from wind, wave, tidal and ocean current energy sources. In particular, a primary goal will be the establishment of a set of equitable and transparent criteria for the evaluation of multi-purpose platforms for marine renewable energy. Using these criteria, a novel system set of design and optimisation tools will be produced addressing new platform design, component engineering, risk assessment, spatial planning, platform-related grid connection concepts, all focussed on system integration and reducing costs. The University of Athens group is in charge for estimation and mapping of wind, wave, tidal and ocean current resources, estimate available energy potential, map extreme event characteristics and provide any additional environmental parameter required.

  15. Imposex and tributyltin contamination as a consequence of the establishment of a marina, and increasing yachting activities at Phuket Island, Thailand.

    Science.gov (United States)

    Bech, Michael

    2002-01-01

    The objective was to investigate how tributyltin (TBT) contamination resulting from the establishment of a new marina and increasing yachting activities at Phuket Island, Thailand affects the area. A minimum of 30 specimen of the mangrove dwelling muricid Chicoreus capucinus were collected at five stations 2 months before and 5, 9, 18 and 33 months after Yacht Haven Marina was constructed. Imposex measured as RPLI and VDSI were significantly correlated to duration of time after construction and distance from the marina. All females developed imposex close to the marina after 18 months, whereas no significant increase occurred at stations more than 2 km from the marina. To evaluate the range of effects of the increasing yachting activities the muricid Thais distinguenda was used as an indicator of imposex at two popular mooring sites for yachts at Phi Phi and Raja Island in Phangna Bay, Thailand. The incidence of imposex was 100% at stations close to the mooring sites. A significant correlation existed between the distance from the sites and the incidence of imposex. The increasing incidence of imposex suggest that TBT contamination is worsening, against global trends, because regulations prohibiting the use of TBT-based paints, do not exist in Thailand.

  16. Primeros registros de babosas marinas (Gastropoda: Heterobranchia del Sistema Arrecifal Tuxpan, México, con ampliaciones de ámbito de distribución

    Directory of Open Access Journals (Sweden)

    Vicencio de la Cruz-Francisco

    2017-06-01

    Full Text Available Se actualiza el conocimiento de las babosas marinas de Veracruz a partir de registros fotográficos. Fundamentalmente se efectuaron muestreos con equipo de buceo libre para áreas someras y buceo autónomo para las pendientes profundas del Sistema Arrecifal Tuxpan (SAT durante el periodo 2014-2016. Se identificaron un total de 11 especies de babosas marinas en el SAT de los cuales Navanax gemmatus y Oxynoe antillarum son nuevos registros para el Sistema Arrecifal Lobos-Tuxpan (SALT, mientras Chelinodura hirundinina, Aplysia morio y Flabellina engeli se registran por primera vez para el estado de Veracruz. Se amplía el ámbito de distribución para el Golfo de México de Thuridilla picta, Tyrinna evelinae, y dos individuos de la familia Phyllidiidae. Estos nuevos registros incrementan el inventario faunístico a 22 especies de babosas marinas para el SALT.

  17. Changes in Organic, Inorganic contents, Carbon Nitrogen ratio in decomposing Avicennia marina and Rhizophora mucronata leaves on tidal mudf lats in Hajambro creek, Indus delta, Pakistan

    Directory of Open Access Journals (Sweden)

    Zafar Farooqui

    2014-01-01

    Full Text Available Leaf decomposition of Avicennia marina (Forskal Vierh in Denkschr and Rhizophora mucronata (Poiret was studied in situ using litterbag s in Hajambro creek, Indus delta. A single exponential model was presented, which best described the rate of decomposition for both the species. The rate of decomposition was species specific, A. marina leaves decomposed slower than the R. mucronata (p<0.001. The time in days required for 50% loss of the initial dry mass (t1/2 was 49.55 days for the A. marina and 44.43 days in case of R. mucronata. The organic content in the leaves was high initially but decresed gradually during decomposition, which is negatively correlated with inorganic contents. This study will help in the management and conservation of mangrove ecosystem of Hajambro creek, Indus delta, Pakistan.

  18. Algunas características de las corrientes marinas en el Golfo de Nicoya, Costa Rica

    Directory of Open Access Journals (Sweden)

    Omar G Lizano

    2004-12-01

    Full Text Available Se estudia la estructura espacial y vertical de las corrientes en el Golfo de Nicoya y su relación con los ciclos de marea usando correntímetros. Se encuentra que en el interior del golfo, la variación vertical de las corrientes marinas aumenta conforme aumenta la profundidad. La estación de Isla Chira muestra un menor grado de variación, tanto en corrientes como en temperatura. La estación entre Isla San Lucas y Puntarenas es la más estratificada en flujos en esta región. Se midieron magnitudes de corrientes sobre los 100 cm/seg durante mareas vivas extraordinarias. Se encontraron desfases del quiebre o cambio de flujo de las mareas entre superficie y fondo, de hasta 100 minutos. En general en esta parte interna los flujos son hacia el interior del Golfo cuando la marea está entrando y hacia el exterior, cuando la marea está bajando. En el exterior del Golfo la circulación es más compleja. A lo largo de un eje Tárcoles-Negritos, se identifican flujos que rotan de manera distinta entre superficie y fondo, cuyos patrones no pueden ser fácilmente explicados según los ciclos de la marea respecto a predicciones mareales en Puntarenas. Estos resultados demuestran que la variación espacial de las corrientes marinas del Golfo de Nicoya está relacionada no solo a la estructura termohalina, sino que también, a los ciclos y ámbitos de las mareas que se producen en este cuerpo de aguaThe spatial and vertical structure of the water currents and its relationship with the tidal cycles were studied using current meters in the Gulf of Nicoya. In the upper gulf, the vertical marine current differences increase as the depth increases. The water column at the station near Chira Island (upper gulf shows the smaller changes in currents and in temperature. The flow at the station between San Lucas Island and Puntarenas (middle gulf is the most stratified in this region. Currents with magnitudes over 100 cm/seg were measured during spring tides

  19. Annual variations of biomass and photosynthesis in Zostera marina at its southern end of distribution in the North Pacific

    Science.gov (United States)

    Cabello-Pasini, Alejandro; Munoz-Salazar, R.; Ward, D.H.

    2003-01-01

    Density, biomass, morphology, phenology and photosynthetic characteristics of Zostera marina were related to continuous measurements of in situ irradiance, attenuation coefficient and temperature at three coastal lagoons in Baja California, Mexico. In situ irradiance was approximately two-fold lower at San Quintin Bay (SQ) than at Ojo de Liebre Lagoon (OL) and San Ignacio Lagoon (SI). As a consequence of the greater irradiance, plants at OL and SI were established 1 m deeper within the water column than those at SQ. At SQ, there was a four-fold variation in biomass of Z. marina caused by changes on shoot length and not shoot density, while at OL and SI biomass and shoot length did not fluctuate significantly throughout the year. Reproductive shoot density reached maximum values concomitantly with the greatest irradiance during spring-summer, however, the density was approximately three-fold greater at SQ than at the southern coastal lagoons. While irradiance levels were two-fold greater at the southern lagoons, in general, photosynthetic characteristics were similar among all three lagoons. The hours of light saturated photosynthesis, calculated from their photosynthetic characteristics and irradiance measurements, suggest that photosynthesis of shoots from OL and SI are saturated for more than 6 h per day throughout the year, while shoots from SQ are likely light limited during approximately 15% of the year. Consequently, an increase in attenuation coefficient values in the water column will likely decrease light availability to Z. marina plants at SQ, potentially decreasing their survival. ?? 2003 Elsevier Science B.V. All rights reserved.

  20. Long-Term Field Study Reveals Subtle Effects of the Invasive Alga Sargassum muticum upon the Epibiota of Zostera marina.

    Science.gov (United States)

    DeAmicis, Stacey; Foggo, Andrew

    2015-01-01

    Invasive species can alter coastal ecosystems both directly, e.g. through competition for substratum and nutrients, and indirectly. Indirect effects may be mediated by creation of dissimilar or inimical habitats, changes in predator and/or prey assemblages, alterations in associated biota, and perturbations of water movement and thermal regimes. Previous studies have shown that invasive algae can modify native habitat architecture, disrupt intricately linked food webs and alter epibiotic assemblages. In the UK, the seagrass Zostera marina supports a diverse epibiotic assemblage, influencing key factors such as sediment dynamics, depositional regime and trophic linkages. Increasing encroachment of the invasive alga Sargassum muticum into seagrass meadows changes the physical and chemical characteristics of the local environment and creates the potential for changes in the epibionts associated with the seagrass blades, threatening the integrity of the seagrass ecosystem. We investigated the effects of S. muticum invasion upon the epibiota of Z. marina in a drowned river valley in SW England seasonally from spring to autumn over four years in an in-situ manipulative experiment, comparing permanent quadrats with and without artificially introduced S. muticum. Epibiota were weighed, identified to the most detailed operational taxonomic unit (OTU) possible, and unitary organisms were enumerated. Multivariate PERMANOVA+ analysis revealed significant differences in epibiont assemblages between Sargassum treatments. Linear mixed effects models indicated that differences in epibiota assemblage composition were not reflected as significant differences in mean biomass per sample, or number of epibiont OTUs per sample. We conclude that S. muticum invasion into Z. marina meadows may significantly alter the species composition and abundance distribution of epibiotic assemblages found on the blades of the seagrass. Thus S. muticum invasion could have more wide-reaching effects on

  1. Aerial root adaptations of Rhizophora apiculata Bl. and Avicennia marina Vierh. to the changes of water levels

    Directory of Open Access Journals (Sweden)

    Bamroongrugsa, N.

    2005-07-01

    Full Text Available Aerial root adaptations of Rhizophora apiculata Bl. and Avicennia marina Vierh. as related to the changes of water levels were carried out on abandoned shrimp ponds of Prince of Songkla University, Pattani Campus, in four water level study plots; 10 cm below soil surface, soil surface level, 10 and 20 cm above soil surface. The results revealed that survival rates of two species were 100 percent in all treatments. Growth of R. apiculata were high in three levels of flooding: soil surface level, 10 and 20 cm above soil surface in the 4th and 8th month. In one year growth of R. apiculata grown in four level of water were slightly different (height: 10 cm below soil surface = 101.35 cm, soil surface level = 108.55 cm, 10 cm above soil surface = 102.20 cm and 20 cm above soil surface = 90.24 cm. The growth of A. marina was highest in 10 cm below soil surface (height = 196.78 cm and growth was high in soil surface level and 10 cm above soil surface (height = 183.66 and 158.53 cm, respectively. The lowest growth was found in 20 cm above soil surface (height = 122.65 cm.. The stilt root of R. apiculata showed good development in two levels of flooding: soil surface level and 10 cm above soil surface, except in circumference of prop root which was biggest in the 20 cm above soil surface. The pneumatophores of A. marina had good development in three water levels: 10 cm below soil surface, soil surface level and 10 cm above soil surface, except in height which was low in 10 cm below soil surface and high in 10 and 20 cm above soil surface. The results of this investigation showed be successfully applied for two mangroves planting and restoration.

  2. Long-Term Field Study Reveals Subtle Effects of the Invasive Alga Sargassum muticum upon the Epibiota of Zostera marina.

    Directory of Open Access Journals (Sweden)

    Stacey DeAmicis

    Full Text Available Invasive species can alter coastal ecosystems both directly, e.g. through competition for substratum and nutrients, and indirectly. Indirect effects may be mediated by creation of dissimilar or inimical habitats, changes in predator and/or prey assemblages, alterations in associated biota, and perturbations of water movement and thermal regimes. Previous studies have shown that invasive algae can modify native habitat architecture, disrupt intricately linked food webs and alter epibiotic assemblages. In the UK, the seagrass Zostera marina supports a diverse epibiotic assemblage, influencing key factors such as sediment dynamics, depositional regime and trophic linkages. Increasing encroachment of the invasive alga Sargassum muticum into seagrass meadows changes the physical and chemical characteristics of the local environment and creates the potential for changes in the epibionts associated with the seagrass blades, threatening the integrity of the seagrass ecosystem. We investigated the effects of S. muticum invasion upon the epibiota of Z. marina in a drowned river valley in SW England seasonally from spring to autumn over four years in an in-situ manipulative experiment, comparing permanent quadrats with and without artificially introduced S. muticum. Epibiota were weighed, identified to the most detailed operational taxonomic unit (OTU possible, and unitary organisms were enumerated. Multivariate PERMANOVA+ analysis revealed significant differences in epibiont assemblages between Sargassum treatments. Linear mixed effects models indicated that differences in epibiota assemblage composition were not reflected as significant differences in mean biomass per sample, or number of epibiont OTUs per sample. We conclude that S. muticum invasion into Z. marina meadows may significantly alter the species composition and abundance distribution of epibiotic assemblages found on the blades of the seagrass. Thus S. muticum invasion could have more wide

  3. Biocalcarenites as construction materials in Santa Marina de Aguas Santas Church at Cordoba, Spain

    Directory of Open Access Journals (Sweden)

    Meroño, J. E.

    2009-03-01

    Full Text Available This study consisted in characterizing the materials used to build Santa Marina de Aguas Santas Church at Cordoba and locating the original quarries. The techniques used in the lithological and chemical characterization included XRD, petrographic microscopy and electron dispersive scanning microscopy. The chemical index of weathering (CIW was used to quantify the state of stone decay. The lithology and different types of alterations observed were mapped. A comparison of the material on the building to ancient quarries identified “Naranjo” as the possible site where the stone was originally quarried.Para la caracterización litológica y determinación del grado de alteración de los materiales pétreos se han empleado las siguientes técnicas: difracción de rayos X (método del polvo, microscopía petrográfica (sobre lámina delgada y microscopía de barrido con EDS (energía dispersiva de rayos X, para determinar la composición química. El estado de degradación del material pétreo se ha cuantificado a partir del índice químico de alteración (CIW. Se han realizado cartografías sobre la fachada oeste: a de las litologías presentes y b de los diferentes tipos de alteración observados. La comparación de muestras del edificio con las de antiguas canteras ha permitido identificar la del Naranjo como la posible cantera de origen.

  4. Dr. Zompo: an online data repository for Zostera marina and Posidonia oceanica ESTs

    Science.gov (United States)

    Wissler, L.; Dattolo, E.; Moore, A. D.; Reusch, T. B. H.; Olsen, J. L.; Migliaccio, M.; Bornberg-Bauer, E.; Procaccini, G.

    2009-01-01

    As ecosystem engineers, seagrasses are angiosperms of paramount ecological importance in shallow shoreline habitats around the globe. Furthermore, the ancestors of independent seagrass lineages have secondarily returned into the sea in separate, independent evolutionary events. Thus, understanding the molecular adaptation of this clade not only makes significant contributions to the field of ecology, but also to principles of parallel evolution as well. With the use of Dr. Zompo, the first interactive seagrass sequence database presented here, new insights into the molecular adaptation of marine environments can be inferred. The database is based on a total of 14 597 ESTs obtained from two seagrass species, Zostera marina and Posidonia oceanica, which have been processed, assembled and comprehensively annotated. Dr. Zompo provides experimentalists with a broad foundation to build experiments and consider challenges associated with the investigation of this class of non-domesticated monocotyledon systems. Our database, based on the Ruby on Rails framework, is rich in features including the retrieval of experimentally determined heat-responsive transcripts, mining for molecular markers (SSRs and SNPs), and weighted key word searches that allow access to annotation gathered on several levels including Pfam domains, GeneOntology and KEGG pathways. Well established plant genome sites such as The Arabidopsis Information Resource (TAIR) and the Rice Genome Annotation Project are interfaced by Dr. Zompo. With this project, we have initialized a valuable resource for plant biologists in general and the seagrass community in particular. The database is expected to grow together with more data to come in the near future, particularly with the recent initiation of the Zostera genome sequencing project. The Dr. Zompo database is available at http://drzompo.uni-muenster.de/ PMID:20157482

  5. Solution structure and excitation energy transfer in phycobiliproteins of Acaryochloris marina investigated by small angle scattering.

    Science.gov (United States)

    Golub, M; Combet, S; Wieland, D C F; Soloviov, D; Kuklin, A; Lokstein, H; Schmitt, F-J; Olliges, R; Hecht, M; Eckert, H-J; Pieper, J

    2017-04-01

    The structure of phycobiliproteins of the cyanobacterium Acaryochloris marina was investigated in buffer solution at physiological temperatures, i.e. under the same conditions applied in spectroscopic experiments, using small angle neutron scattering. The scattering data of intact phycobiliproteins in buffer solution containing phosphate can be well described using a cylindrical shape with a length of about 225Å and a diameter of approximately 100Å. This finding is qualitatively consistent with earlier electron microscopy studies reporting a rod-like shape of the phycobiliproteins with a length of about 250 (M. Chen et al., FEBS Letters 583, 2009, 2535) or 300Å (J. Marquart et al., FEBS Letters 410, 1997, 428). In contrast, phycobiliproteins dissolved in buffer lacking phosphate revealed a splitting of the rods into cylindrical subunits with a height of 28Å only, but also a pronounced sample aggregation. Complementary small angle neutron and X-ray scattering experiments on phycocyanin suggest that the cylindrical subunits may represent either trimeric phycocyanin or trimeric allophycocyanin. Our findings are in agreement with the assumption that a phycobiliprotein rod with a total height of about 225Å can accommodate seven trimeric phycocyanin subunits and one trimeric allophycocyanin subunit, each of which having a height of about 28Å. The structural information obtained by small angle neutron and X-ray scattering can be used to interpret variations in the low-energy region of the 4.5K absorption spectra of phycobiliproteins dissolved in buffer solutions containing and lacking phosphate, respectively. Copyright © 2017 Elsevier B.V. All rights reserved.

  6. The stable carbon isotope ratio of some components of an eelgrass, Zostera marina, bed.

    Science.gov (United States)

    Thayer, Gordon W; Parker, Patrick L; LaCroix, Michael W; Fry, Brian

    1978-01-01

    Living and dead Zostera marina blades, plankton samples, sediments, and several animal components of an eelgrass bed near Beaufort, N.C. were collected and analyzed for 13C/12C ratios (δ 13C). The δ 13C values of producer and consumer organisms were compared in order to examine the possible origins of organic matter present in the consumers. Living and dead eelgrass blades displayed similar δ13C values,-12.2 and-10.6 per mil ‰ respectively, while the epiphytic community growing on the grass blades had a mean isotope ratio of-16.0‰. Animal components analyzed represented five major feeding-mode categories: invertebrates living on grass blades an presumably feeding on the epibiota (-15.1‰), deposit feeding invertebrates (-15.0‰), predatory and omnivorous invertebrates (-16.7‰), suspension and surface feeding invertebrates (-18.3‰) and omnivorous fish (-16.8‰).Organisms commonly found on the grass blades appeared to feed primarily on the epibiota growing on the blades. It is hypothesized that the epibiota derive some of their carbon from DOC released by the Zostera blade. The urchin, Lytechinus variegatus, and the brittle star, Ophioderma brevispinum, both deposit feeders, appeared to derive a major proportion of their carbon from eelgrass. With the exception of the shrimp, Alpheus heterochaelis, and the pipefish, Syngnathus floridae, the majority of other organisms analyzed appeared to be linked more directly to a plankton-carbon food chanin than to a seagrass-carbon system in this relatively young eelgrass bed.

  7. Zostera marina seed burial can be enhanced by Manila clam Ruditapes philippinarum: A microcosm study

    Science.gov (United States)

    Li, Chang-Jun; Li, Wen-Tao; Liu, Jianying; Zhang, Xiumei; Zhang, Peidong

    2017-06-01

    Seagrass seed bank plays a key role in the regeneration of new vegetation when seagrasses are removed by the natural or man-made disaster. Various factors may affect the development of sediment seed bank. We conducted a microcosm experiment to test the effects of burrowing and feeding activities of Manila clam, Ruditapes philippinarum on the burial of Zostera marina seeds in sediments. The effects of lasting time (3-hour, 1-day, 3-day, 7-day, 14-day and 28-day), clam density (0, 2, 4 and 8 clams with shell length of 3 cm in each microcosm) and clam size (shell length of 2, 3 and 4 cm at 4-clam density) on seed burial were examined in plastic microcosm cores (30 cm high × 10 in inner diameter) in a 28-day period. Results showed that the seed burial depth significantly increased with time, the density and the size of clams. No seeds were buried in the sediment in the cores without clams during the whole experiment period. For the 3-cm clams, about 91.61% of the seeds were buried in the sediment at the end of the experiment in the high-density treatment (8 clams at each core); while in the medium and low-density treatments (4 and 2 clams in each core, respectively), about 76.93% and 60.61% of the seeds were buried in the sediment, respectively. For the size treatments, large (4 cm) clams buried 89.56% of the seeds at the end of the experiment, much more than those of medium (3 cm, 76.93%) and small (2 cm, 61.50%) size clams. During the whole experiment period, nearly all of the buried seeds were at a depth of from 0 cm to 5 cm. These results suggested that Manila clam Ruditapes philippinarum may play an important positive role in seagrass seed bank dynamics in the field.

  8. Indice de la microflora marina de Venezuela: diatomeas, dinoflagelados y cocolitofóridos

    Directory of Open Access Journals (Sweden)

    José Rafael Díaz-Ramos

    2000-12-01

    Full Text Available Los estudios sobre el fitoplancton marino de Venezuela han sido realizados de manera regular desde mediados del siglo XX. Sin embargo, hasta ahora no se ha realizado un compendio de las especies encontradas que sirva como marco de referencia a los trabajos taxonómicos. En este trabajo se presenta por primera vez un índice de la microflora marina de Venezuela. El índice incluye sólo las diatomeas (89 especies céntricas y 186 especies pennadas, los dinoflagelados (ocho especies atecadas y 154 especies tecadas y los cocolitóforidos (24 especies a los cuales se les realizado una diagnosis formal e ilustraciones (dibujos y/o fotografias y que han sido reportados en la literatura científica (revistas y/o tesis de licenciatura o maestría. Esta ordenado según la clase, orden, familia y especie. Además, en el se cita al autor (es de cada taxón.ha.The marine phytoplankton of Venezuela has been studied on a regular basis since the mid 20th century. However, a species checklist that can be used as a framework for taxonomic studies is lacking. In this paper, an index of the marine microflora of Venezuela is presented for the first time. The index includes only those diatoms (89 centric and 186 pennate species, dinoflagellates (eight naked and 154 thecate species and coccolithophores (24 especies for which formal diagnosis and illustrations (drawings and/or photographs have been reported in the scientific literature (journals and/or first degree or master's theses. It is ordered alphabetically according to class, order, family, and species. It includes the author (s of the taxa.

  9. Understanding Aquaporin Transport System in Eelgrass (Zostera marina L., an Aquatic Plant Species

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    S. M. Shivaraj

    2017-08-01

    Full Text Available Aquaporins (AQPs are a class of integral membrane proteins involved in the transport of water and many other small solutes. The AQPs have been extensively studied in many land species obtaining water and nutrients from the soil, but their distribution and evolution have never been investigated in aquatic plant species, where solute assimilation is mostly through the leaves. In this regard, identification of AQPs in the genome of Zostera marina L. (eelgrass, an aquatic ecological model species could reveal important differences underlying solute uptake between land and aquatic species. In the present study, genome-wide analysis led to the identification of 25 AQPs belonging to four subfamilies, plasma membrane intrinsic proteins (PIPs, tonoplast intrinsic proteins (TIPs, nodulin 26-like intrinsic proteins (NIPs, small basic intrinsic proteins (SIPs in eelgrass. As in other monocots, the XIP subfamily was found to be absent from the eelgrass genome. Further classification of subfamilies revealed a unique distribution pattern, namely the loss of the NIP2 (NIP-III subgroup, which is known for silicon (Si transport activity and ubiquitously present in monocot species. This finding has great importance, since the eelgrass population stability in natural niche is reported to be associated with Si concentrations in water. In addition, analysis of available RNA-seq data showed evidence of expression in 24 out of the 25 AQPs across four different tissues such as root, vegetative tissue, male flower and female flower. In contrast to land plants, higher expression of PIPs was observed in shoot compared to root tissues. This is likely explained by the unique plant architecture of eelgrass where most of the nutrients and water are absorbed by shoot rather than root tissues. Similarly, higher expression of the TIP1 and TIP5 families was observed specifically in male flowers suggesting a role in pollen maturation. This genome-wide analysis of AQP distribution

  10. FLORA MARINA BENTÓNICA DE LA REGIÓN AUSTRAL DE SUDAMÉRICA Y LA ANTÁRTICA

    OpenAIRE

    Ramírez,María Eliana

    2010-01-01

    El conocimiento taxonómico de la flora marina bentónica de la región austral de Sudamérica, incluida la Antártica, está basado fundamentalmente en los registros de las expediciones europeas que recorrieron los mares australes durante los siglos XVIII, XIX y principios del siglo XX. Debido a la lejanía de estos territorios y a las dificultades logísticas para la realización de nuevas recolecciones en el área, poco se ha avanzado en la revisión crítica de los taxa publicados en la literatura ha...

  11. Contribución al estudio químico de tres especies de algas marinas peruanas

    OpenAIRE

    Acha de la Cruz, Otilia

    1995-01-01

    En el presente trabajo de Investigación, se hicieron estudios químicos en tres especies de algas marinas peruanas. En la especie Macrocystis piryfera se hicieron estudios de compuestos orgánicos e inorgánicos. Respecto a los compuestos inorgánicos, se determinó un gran contenido de minerales, (esta especie es un suplemento ideal para fertilizantes en la agricultura). Respecto a los compuestos orgánicos se hizo un estudio con variación estacional del contenido del ácido algínico, mani...

  12. Niveles de plomo y cadmio en agua marina y lapas (Patella vulgata L.) de la Ría de Vigo

    OpenAIRE

    M Pérez López; M.C Nóvoa; Alonso, J.; M. A. García Fernández; M.J. Melgar

    2003-01-01

    El empleo de seres vivos para monitorizar la contaminación por metales pesados en los ecosistemas acuáticos es de extremo interés en el campo de la ecotoxicología. En el presente estudio se han recogido muestras de agua marina y de lapas (Patella vulgata L.) de distintos puntos de la Ría de Vigo, con la intención de determinar en ellos los niveles de plomo y cadmio, por medio de una técnica de voltamperometría. Los resultados obtenidos mostraron una relación estrecha entr...

  13. La industria prehispánica de conchas marinas en "Gran Coclé", Panamá

    OpenAIRE

    Mayo Torné, Julia del Carmen

    2004-01-01

    Las sociedades americanas prehispánicas sintieron desde muy temprano un aprecio especial por las conchas como materia prima. Con ellas elaboraron pequeñas y exquisitas obras de arte, adornos o ajuares funerarios, e incluso en ocasiones ciertas especies alcanzaron un alto valor simbólico. En algunos casos, cantidades significativas de conchas marinas aparecen en talleres. Los análisis y estudios de los restos abandonados en estos talleres están orientados a d efinir las técnicas de manufactura...

  14. Consumo de algas marinas: su influencia en el valor nutritivo de la dieta y en diversos parámetros fisiológicos

    OpenAIRE

    Millán Lence, Rosendo

    2016-01-01

    En general, las algas marinas son una buena fuente de fibra al mismo tiempo que proporcionan vitaminas, minerales y nutracéuticos de interés para la alimentación humana. Por ello, en este estudio se analiza cómo influye la ingesta de las algas marinas Porphyra purpurea, Undaria pinnatifida, Himanthalia elongata, Ulva rigida y Codium tomentosum en la calidad nutritiva del alimento y en diversos parámetros hemáticos. Debido a que las enzimas implicadas en los procesos digestivos se adaptan a la...

  15. Influencia dos ingredientes utilizados no processo de marinação sobre a aparencia global de lagarto (M. semitendinosus) bovino marinado

    OpenAIRE

    Bruce Fonseca Mota

    2005-01-01

    Resumo: O presente trabalho foi dividido em duas etapas. Na primeira etapa avaliou-se o efeito dos ingredientes (sal, tripolifosfato de sódio, vinagre, alho e cebola em pó e maltodextrina), presentes em diferentes formulações de salmoura de marinação sobre aparência de lagarto (M. semitendinosus) bovino injetado com estas salmouras segundo um planejamento fatorial fracionário 2 5-1. Também foramanalisados o rendimento do processo de marinação e o pH dos cortes cárneos, para que se tentasse co...

  16. Identificación de las prioridades de conservación de la biodiversidad marina y costera en Costa Rica

    Directory of Open Access Journals (Sweden)

    Juan José Alvarado

    2011-06-01

    Full Text Available Costa Rica es un país reconocido por su alta diversidad de especies y ecosistemas, tanto en sus ambientes terrestres como marinos. A pesar de esta importancia, presenta un rezago en la conservación y manejo de la biodiversidad marina y costera, con respecto a la terrestre. Para el año 2006, la superficie marina protegida era de 5 208.8km² y 331.5km de línea costera, en 20 áreas silvestres protegidas. El país ha logrado importantes avances en la selección de sitios prioritarios para la conservación de la biodiversidad terrestre y de aguas continentales, aunque pocos esfuerzos se han realizado hasta el momento en la planificación marina. En este trabajo se muestra el análisis y resultados de un proceso de identificación de vacíos en la representatividad de la biodiversidad marina y costera en el sistema de áreas protegidas de Costa Rica. El estudio se construyó a partir de la información espacial disponible sobre la presencia y distribución de la biodiversidad marina y costera, el establecimiento de metas de conservación y el análisis de amenazas sobre la integridad ecológica de la biodiversidad. Posteriormente, se llevó a cabo la selección de sitios prioritarios mediante técnicas de optimización espacial, y la sobreposición con la actual capa de áreas marinas protegidas, para finalmente identificar los vacíos en la representatividad. En total, se identificaron 19 076km² de vacíos de conservación, 1 323km² en el Caribe y 17 753km² en el Pacífico. Se plantean recomendaciones dirigidas hacia la ampliación y fortalecimiento del sistema de áreas marinas protegidas del país, en el cual se usan los vacíos identificados como marco de referencia. Se espera que los resultados presentes sean parte de la base científica necesaria para la planificación de la conservación y uso sostenible de la biodiversidad marina en el país.

  17. Liver histopathology in the cane toad, Rhinella marina (Amphibia: Bufonidae), induced by Ortleppascaris sp. larvae (Nematoda: Ascarididae).

    Science.gov (United States)

    Silva, Jefferson P E; da Silva, Djane C B; Melo, Francisco T V; Giese, Elane G; Furtado, Adriano P; Santos, Jeannie N

    2013-04-01

    Exposure to parasites is considered to be an important factor in the development of many diseases and histopathologies which are the result of the parasite-host interaction. The present study evaluated the impact of natural infection by larvae of Ortleppascaris sp. (Nematoda: Ascaridida) in the liver of the cane toad Rhinella marina (Linnaeus, 1758). Larvae were encysted in nodules delimited by collagenous fibers and fibroblasts or freely within the hepatic parenchyma, provoking a clear response from the host. The histological examination of the liver revealed viable larvae in a number of different developmental stages, as well as cysts filled with amorphous material and cell residues and surrounded by dense fibrotic tissue. The infection of the liver by these larvae induces a significant increase in the area occupied by melanomacrophages and a reduction or deficit in the vascularization of the liver, hypertrophy of the hepatocytes, vacuolar bodies, and cytoplasmatic granules. Focal concentrations of inflammatory infiltrates were observed enclosing the unencapsulated early-stage larvae. These results indicate that infection by Ortleppascaris sp. induces severe physiological problems and histopathological lesions in the liver of R. marina .

  18. Population-specificity of heat stress gene induction in northern and southern eelgrass Zostera marina populations under simulated global warming.

    Science.gov (United States)

    Bergmann, Nina; Winters, Gidon; Rauch, Gisep; Eizaguirre, Christophe; Gu, Jenny; Nelle, Peter; Fricke, Birgit; Reusch, Thorsten B H

    2010-07-01

    Summer heat waves have already resulted in mortality of coastal communities, including ecologically important seagrass meadows. Gene expression studies from controlled experiments can provide important insight as to how species/genotypes react to extreme events that will increase under global warming. In a common stress garden, we exposed three populations of eelgrass, Zostera marina, to extreme sea surface temperatures, simulating the 2003-European heat wave. Populations came from locations widely differing in their thermal regime, two northern European locations [Ebeltoft (Kattegat), Doverodde (Limfjord, Baltic Sea)], and one southern population from Gabicce Mare (Adriatic Sea), allowing to test for population specificity in the response to a realistic heat stress event. Eelgrass survival and growth as well as the expression of 12 stress associated candidate genes were assessed during and after the heat wave. Contrary to expectations, all populations suffered equally from 3 weeks of heat stress in terms of shoot loss. In contrast, populations markedly differed in multivariate measures of gene expression. While the gene expression profiles converged to pre-stress values directly after the heat wave, stress correlated genes were upregulated again 4 weeks later, in line with the observed delay in shoot loss. Target genes had to be selected based on functional knowledge in terrestrial plants, nevertheless, 10/12 genes were induced relative to the control treatment at least once during the heat wave in the fully marine plant Z. marina. This study underlines the importance of realistic stress and recovery scenarios in studying the impact of predicted climate change.

  19. Identification and kinetic characterization of a novel superoxide dismutase from Avicennia marina: An antioxidant enzyme with unique features.

    Science.gov (United States)

    Zeinali, Farrokhzad; Homaei, Ahmad; Kamrani, Ehsan

    2017-12-01

    A novel Cu/Zn-superoxide dismutase was extracted from Avicennia marina and purified. The sample was collected from Khamir port located in the north shore of Persian Gulf. The purification procedure comprised of (NH4)2SO4 precipitation followed by CM-Sephadex C-50 and DEAE-Sepharose chromatography, and gel filtration chromatography (Sephadex G-75). The enzyme with a characteristic molecular weight of 31kDa, measured by SDS-page, showed its highest catalytic efficiency at pH 8.0 and 50°C. Its activity was greatly inhibited by cyanide and hydrogen peroxide. The pH profile showed that the enzyme could maintain most of its activity at pH values ranging from 5 to 10. The temperature profile of this enzyme showed a broad range of activity compared with other superoxide dismutases. Catalytic hydrolysis rate followed Michaelis-Menten equation. The values of kcat and Km were obtained from Michaelis-Menten plot as 107000s(-1) and 11.5μmol respectively. The evidences from kinetic and thermodynamic parameters suggest that Avicennia marina superoxide dismutase (AmSOD) can be used as a suitable enzyme for biotechnological and pharmacological applications. Copyright © 2017 Elsevier B.V. All rights reserved.

  20. Characterization of a New Trioxilin and a Sulfoquinovosyl Diacylglycerol with Anti-Inflammatory Properties from the Dinoflagellate Oxyrrhis marina

    Directory of Open Access Journals (Sweden)

    Eun Young Yoon

    2017-02-01

    Full Text Available Two new compounds—a trioxilin and a sulfoquinovosyl diacylglycerol (SQDG—were isolated from the methanolic extract of the heterotrophic dinoflagellate Oxyrrhis marina cultivated by feeding on dried yeasts. The trioxilin was identified as (4Z,8E,13Z,16Z,19Z -7(S,10(S,11(S-trihydroxydocosapentaenoic acid (1, and the SQDG was identified as (2S-1-O-hexadecanosy-2-O-docosahexaenoyl-3-O-(6-sulfo-α-d-quinovopyranosyl-glycerol (2 by a combination of nuclear magnetic resonance (NMR spectra, mass analyses, and chemical reactions. The two compounds were associated with docosahexaenoic acid, which is a major component of O. marina. The two isolated compounds showed significant nitric oxide inhibitory activity on lipopolysaccharide-induced RAW264.7 cells. Compound 2 showed no cytotoxicity against hepatocarcinoma (HepG2, neuroblastoma (Neuro-2a, and colon cancer (HCT-116 cells, while weak cytotoxicity was observed for compound 1 against Neuro-2a cells.

  1. Survey of helminths, ectoparasites, and chytrid fungus of an introduced population of cane toads, Rhinella marina (Anura: Bufonidae), from Grenada

    Science.gov (United States)

    Drake, Michael C.; Zieger, Ulrike; Groszkowski, Andrew; Gallardo, Bruce; Sages, Patti; Reavis, Roslyn; Faircloth, Leslie; Jacobson, Krystin; Lonce, Nicholas; Pinckney, Rhonda D.; Cole, Rebecca A.

    2014-01-01

    One hundred specimens of Rhinella marina, (Anura: Bufonidae) collected in St. George's parish, Grenada, from September 2010 to August 2011, were examined for the presence of ectoparasites and helminths. Ninety-five (95%) were parasitized by 1 or more parasite species. Nine species of parasites were found: 1 digenean, 2 acanthocephalans, 4 nematodes, 1 arthropod and 1 pentastome. The endoparasites represented 98.9% of the total number of parasite specimens collected. Grenada represents a new locality record for Mesocoelium monas, Raillietiella frenatus, Pseudoacanthacephalus sp., Aplectana sp., Physocephalus sp., Acanthacephala cystacanth, and Physalopteridae larvae. The digenean M. monas occurred with the highest prevalence of 82%, contrasting many studies of R. marina where nematodes dominate the parasite infracommunity. Female toads were found to have a significantly higher prevalence of Amblyomma dissimile than male toads. Only 2 parasites exhibited a significant difference between wet and dry season with Parapharyngodon grenadensis prevalence highest in the wet season and A. dissimile prevalence highest during the dry season. Additionally, A. dissimilewas significantly more abundant during the dry season.

  2. The Structure of Genetic Diversity in Eelgrass (Zostera marina L.) along the North Pacific and Bering Sea Coasts of Alaska

    Science.gov (United States)

    Talbot, Sandra; Sage, Kevin; Rearick, Jolene; Fowler, Megan C.; Muñiz-Salazar, Raquel; Baibak, Bethany; Wyllie-Echeverria, Sandy; Cabello-Pasini, Alehandro; Ward, David H.

    2016-01-01

    Eelgrass (Zostera marina) populations occupying coastal waters of Alaska are separated by a peninsula and island archipelago into two Large Marine Ecosystems (LMEs). From populations in both LMEs, we characterize genetic diversity, population structure, and polarity in gene flow using nuclear microsatellite fragment and chloroplast and nuclear sequence data. An inverse relationship between genetic diversity and latitude was observed (heterozygosity: R2 = 0.738, P < 0.001; allelic richness: R2 = 0.327, P = 0.047), as was significant genetic partitioning across most sampling sites (θ = 0.302, P < 0.0001). Variance in allele frequency was significantly partitioned by region only in cases when a population geographically in the Gulf of Alaska LME (Kinzarof Lagoon) was instead included with populations in the Eastern Bering Sea LME (θp = 0.128–0.172; P < 0.003), suggesting gene flow between the two LMEs in this region. Gene flow among locales was rarely symmetrical, with notable exceptions generally following net coastal ocean current direction. Genetic data failed to support recent proposals that multiple Zostera species (i.e. Z. japonica and Z. angustifolia) are codistributed with Z. marina in Alaska. Comparative analyses also failed to support the hypothesis that eelgrass populations in the North Atlantic derived from eelgrass retained in northeastern Pacific Last Glacial Maximum refugia. These data suggest northeastern Pacific populations are derived from populations expanding northward from temperate populations following climate amelioration at the terminus of the last Pleistocene glaciation.

  3. Impact of polychaetes (Nereis spp. and Arenicola marina on carbon biogeochemistry in coastal marine sediments†

    Directory of Open Access Journals (Sweden)

    Kristensen Erik

    2001-10-01

    Full Text Available Known effects of bioturbation by common polychaetes (Nereis spp. and Arenicola marina in Northern European coastal waters on sediment carbon diagenesis is summarized and assessed. The physical impact of irrigation and reworking activity of the involved polychaete species is evaluated and related to their basic biology. Based on past and present experimental work, it is concluded that effects of bioturbation on carbon diagenesis from manipulated laboratory experiments cannot be directly extrapolated to in situ conditions. The 45–260% flux (e.g., CO2 release enhancement found in the laboratory is much higher than usually observed in the field (10–25%. Thus, the faunal induced enhancement of microbial carbon oxidation in natural sediments instead causes a reduction of the organic matter inventory rather than an increased release of CO2 across the sediment/water interface. The relative decrease in organic inventory (Gb/Gu is inversely related to the relative increase in microbial capacity for organic matter decay (kb/ku. The equilibrium is controlled by the balance between organic input (deposition of organic matter at the sediment surface and the intensity of bioturbation. Introduction of oxygen to subsurface sediment and removal of metabolites are considered the two most important underlying mechanisms for the stimulation of carbon oxidation by burrowing fauna. Introduction of oxygen to deep sediment layers of low microbial activity, either by downward irrigation transport of overlying oxic water or by upward reworking transport of sediment to the oxic water column will increase carbon oxidation of anaerobically refractory organic matter. It appears that the irrigation effect is larger than and to a higher degree dependent on animal density than the reworking effect. Enhancement of anaerobic carbon oxidation by removal of metabolites (reduced diffusion scale may cause a significant increase in total sediment metabolism. This is caused by three

  4. New evidence for habitat-specific selection in Wadden Sea Zostera marina populations revealed by genome scanning using SNP and microsatellite markers

    NARCIS (Netherlands)

    Oetjen, Katharina; Ferber, Steven; Dankert, Ilka; Reusch, Thorsten B. H.

    Eelgrass Zostera marina is an ecosystem-engineering species of outstanding importance for coastal soft sediment habitats that lives in widely diverging habitats. Our first goal was to detect divergent selection and habitat adaptation at the molecular genetic level; hence, we compared three pairs of

  5. Leaf water storage increases with salinity and aridity in the mangrove Avicennia marina: integration of leaf structure, osmotic adjustment and access to multiple water sources.

    Science.gov (United States)

    Nguyen, Hoa T; Meir, Patrick; Sack, Lawren; Evans, John R; Oliveira, Rafael S; Ball, Marilyn C

    2017-08-01

    Leaf structure and water relations were studied in a temperate population of Avicennia marina subsp. australasica along a natural salinity gradient [28 to 49 parts per thousand (ppt)] and compared with two subspecies grown naturally in similar soil salinities to those of subsp. australasica but under different climates: subsp. eucalyptifolia (salinity 30 ppt, wet tropics) and subsp. marina (salinity 46 ppt, arid tropics). Leaf thickness, leaf dry mass per area and water content increased with salinity and aridity. Turgor loss point declined with increase in soil salinity, driven mainly by differences in osmotic potential at full turgor. Nevertheless, a high modulus of elasticity (ε) contributed to maintenance of high cell hydration at turgor loss point. Despite similarity among leaves in leaf water storage capacitance, total leaf water storage increased with increasing salinity and aridity. The time that stored water alone could sustain an evaporation rate of 1 mmol m -2  s -1 ranged from 77 to 126 min from subspecies eucalyptifolia to ssp. marina, respectively. Achieving full leaf hydration or turgor would require water from sources other than the roots, emphasizing the importance of multiple water sources to growth and survival of Avicennia marina across gradients in salinity and aridity. © 2017 John Wiley & Sons Ltd.

  6. MAPPING INTERTIDAL EELGRASS (ZOSTERA MARINA L.) IN THREE COASTAL ESTUARIES OF THE PACIFIC NORTHWEST USA USING FALSE-COLOUR NEAR-INFRARED AERIAL PHOTOGRAPHY

    Science.gov (United States)

    This study describes a hybrid technique of digitally classifying aerial photography used for mapping the intertidal habitat of eelgrass (Zostera marina L.) in Pacific Northwest USA estuaries. The large tidal range (2-3 m) in this region exposes most of this seagrass community at ...

  7. Biogeografía marina de Chile continental Marine biogeography of continental Chile

    Directory of Open Access Journals (Sweden)

    PATRICIO A. CAMUS

    2001-09-01

    Full Text Available Por casi un siglo, varios autores han analizado los patrones de distribución de la fauna y flora marina del Pacífico de Sudamérica y de la costa de Chile en particular, con el propósito de establecer el número de las unidades biogeográficas presentes. Si bien los patrones generales actuales son relativamente bien conocidos, la mayoría de los estudios se ha enfocado en las discontinuidades distribucionales y en propuestas de clasificación más que en los factores y procesos dinámicos que han formado las unidades espaciales identificadas. Aun en el caso de las clasificaciones, las conclusiones publicadas se basan principalmente en el análisis de uno o pocos grupos seleccionados, y presentan diferencias importantes en los criterios de selección de los grupos, el número de especies involucrado y la metodología usada. Por otra parte, las discrepancias entre estudios específicos tienden a obscurecer un fenómeno relevante y de mayor escala como es la dinámica de las biotas, uno de los aspectos biogeográficos menos conocidos en Chile. En tal contexto, este trabajo presenta una revisión de literatura sobre la biogeografía marina de Chile y sus aspectos asociados, con los siguientes objetivos: (a resumir las características oceanográficas, climáticas y geomorfológicas de la costa continental de Chile; (b discutir 27 clasificaciones biogeográficas publicadas para la costa chilena, analizando los criterios y procedimientos usados por los autores, sus conclusiones principales, y la concordancia entre los estudios; (c evaluar los procesos dispersivos y vicariantes asociados a los desplazamientos y modificaciones de las biotas de la región, en función de los antecedentes disponibles sobre las condiciones existentes y los principales eventos ocurridos durante los períodos Terciario y Cuaternario; y (d proponer un escenario de cambio biogeográfico basado en determinantes históricas y su influencia en la formación, carácter y din

  8. REPETITION AS A SPECIAL TYPE OF SYNTACTIC RELATIONS IN REPRESENTED SPEECH: BASED ON THE PROSE BY MARINA TSVETAEVA

    Directory of Open Access Journals (Sweden)

    Olga Pavlovna Puchinina

    2017-03-01

    Full Text Available The author studies the peculiarities of repetition, being one of the ways to build syntactic relations in the represented speech structure, based on the prose by Marina Tsvetaeva. The article describes the different types of repetitions, their distinctive features and frequency of usage in the studied tests. The author describes different types of repetitions in the studied cases of represented speech from the point of view of their position. Repetition occurs at the beginning, end of sentences, beginning and end of a statement or paragraph, end of a statement and beginning of the next one; lexical items may be repeated in the middle of a statement. The morphology of repetitions, i.e., the way by what parts of speech the repeated words and structures are expressed, is of interest from the point of view of functional grammar. The author notes that Tsvetaeva repeats different parts of speech: conjunctions, prepositions, particles, nouns, pronouns, adverbs, numerals, verbs, modal words or a combination of two words. Moreover, due to her special intention, Tsvetaeva intensifies repetition through particular phonetic devices, such as alliteration, rhyme and rhythm, which make her prosaic works sound poetic. Purpose. The article is devoted to the topic of rendering another person’s speech, as it continues to be one of the most important issues of modern linguistics. The subject of analysis is repetition and its different types in the structure of represented speech on the material of prose texts by Marina Tsvetaeva. The author’s aims is to reveal the way these types of repetition (lexical, syntactic, semantic ones function in the structure of represented speech and what effect is achieved with their help. Methodology. The research has been conducted using the continuous sampling method and the quantitative estimation method, aimed to identify the frequency of using different types of repetition and repeated parts of speech and constructions in the

  9. Low absorption state of phycocyanin from Acaryochloris marina antenna system: On the interplay between ionic strength and excitonic coupling

    Science.gov (United States)

    Nganou, Collins

    2013-07-01

    This paper studies the excitonic factor in the excited state energy transfer of phycobilisome (PBS) by using a polarized time-resolved pump-probe and by changing the ionic strength of the cofactors' medium in the PBS of Acaryochloris marina (A. marina). As a result, the interplay between the surrounding medium and the closely excited adjacent cofactors is shown to be a negligible factor of the excitonic decay kinetics at 618 nm of the phycocyanin (PC), while it appears as a driving factor of an increase in excitonic delocalization at 630 nm. The obtained anisotropy values are consistent with the contribution of ionic strength in the excitonic mechanism in PBS. These values were 0.38 in high ionic strength and 0.4 in low ionic strength at 618 nm, and 0.52 in high ionic strength and 0.4 in low ionic strength at 630-635 nm. The anisotropy value of 0.52 in high phosphate is similar at 630 nm and 635 nm, which is consistent with an excitonic delocalization band at 635 nm. The 635 nm band is suggested to show the true low energy level of PC in A. marina PBS. The anisotropy decay kinetic at 630 nm suggests that the excited state population of PC is not all equilibrated in 3 ps because of the existence of the 10 ps decay kinetic component. The presence of the slow kinetic decay component in high, and low ionic strength, is consistent with a 10 and 14 ps energy transfer pathway, while the 450 fs kinetic decay component is consistent with the presence of an additional excitation energy transfer pathway between adjacent α84 and β84. Furthermore, the 450 fs decay kinetic is suggested to be trapped in the trimer, while the 400 fs decay kinetic rules out an excitonic flow from low energy level PC to allophycoyanin. This excitonic flow may occur between β84 in adjacent trimers, towards the low energy state of the PBS rod.

  10. Karl Lenz, Marina Adler: Geschlechterverhältnisse. Einführung in die sozialwissenschaftliche Geschlechterforschung Band 1. Weinheim u.a.: Juventa Verlag 2010. — Karl Lenz, Marina Adler: Geschlechterbeziehungen. Weinheim u.a.: Juventa Verlag 2011.

    Directory of Open Access Journals (Sweden)

    Heike Kahlert

    2012-05-01

    Full Text Available Karl Lenz und Marina Adler orientieren sich in ihrem zweibändigen Lehrbuch zur sozialwissenschaftlichen Geschlechterforschung an der etablierten Unterscheidung zwischen Makro- und Mikrosoziologie. Im ersten Band werden theoretische Perspektiven der Geschlechterforschung und unterschiedliche Zusammenhänge zu Kultur nachgezeichnet sowie zentrale Themenfelder einer Sozialstrukturanalyse der Geschlechterordnung fokussiert, nämlich Recht, Politik, Bildung und Arbeit. Im zweiten Band stehen neben der Sozialisation ausgewählte Erträge der mikrosoziologischen Geschlechterforschung im Zentrum, nämlich Körper, Sexualität, persönliche Beziehungen, Devianz und Gewalt. In dieser überfälligen, informativen, sorgfältig recherchierten, aber auch vergleichsweise traditionellen und empirisch orientierten Einführung wird die Geschlechterforschung zwar in einer breiteren Diversity-Forschung verortet, das Verhältnis von Diversität und Geschlecht leider aber nicht reflektiert.In their two-volume textbook on social-scientific gender studies, Karl Lenz and Marina Adler are guided by the established distinction between macro- and microsociology. The first volume traces theoretical perspectives on gender studies and various connections to culture. Furthermore, it also zeroes in on central topics of a social structure analysis of the gender order, namely law, politics, education, and work. The second volume focuses on socialization as well as selected findings of microsociological gender studies, namely body, sexuality, personal relationships, deviance, and violence. This overdue, informative, carefully researched, but comparatively traditional and empirically oriented introduction locates gender studies within the broader field of diversity research; however, the relationship between diversity and gender is unfortunately not reflected on.

  11. ANÁLISIS ESPECTRAL DE OLAS MARINAS: MODELOS UNIVARIADOS // SPECTRAL ANALYSIS OF WAVE SEA: UNIVARIATE MODELS

    Directory of Open Access Journals (Sweden)

    Nestor Escudero Mora

    2015-12-01

    Full Text Available El análisis espectral, es el proceso técnico de la descomposición de una señal compleja en partes más simples. Muchos procesos físicos se describen mejor como una suma de muchas componentes de frecuencias individuales, alternativamente una señal puede dividirse en segmentos cortos y el análisis del espectro puede aplicarse a estos segmentos individuales. Desde el punto de vista del estudio de olas marinas, el espectro juega un rol fundamental y es interpretado como la energía presente en la serie de tiempo durante el periodo. Este estudio se basa en el análisis del espectro asociado a datos del mar del Norte en el ano de 1999 en la plataforma North Alwyn almacenados en la Universidad Heriot-Watt de Edimburgo, separados en periodos de 20 minutos, el total a estudiar son 244 periodos. Para ello, se determinó que existe información que no aporta al problema, representa ruido y es el 3% de la energía, la cual no fue tomada para el estudio. Se dividió el espectro en 12 subintervalos y se ajustaron modelos autorregresivos bajo el esquema de Box-Jenskin and Reinsel, estudiados estos hasta encontrar la mejor aproximación posible para cada subintervalo mediante las propiedades estadísticas obtenidas por cada modelo, junto con los pronósticos respectivos. // ABSTRACT: Spectral analysis is the technical process of a complex signal decomposition into simpler parts. Many physical processes are better described as a sum of many individual frequency components; alternatively a signal can be divided into short segments and the spectrum analysis can be applied to these individual segments. From the point of view of the study of Waves Sea, the spectrum plays a vital role and it is interpreted as the energy in the time series during the period. This study is based on analysis of the spectrum associated to data from the North Sea in the year of 1999, taken in North Alwyn platform, stored in the Heriot-Watt University in Edinburgh, separated in

  12. Accumulation of trace metals in grey mangrove Avicennia marina fine nutritive roots: the role of rhizosphere processes.

    Science.gov (United States)

    Chaudhuri, Punarbasu; Nath, Bibhash; Birch, Gavin

    2014-02-15

    Mangrove sediment has long been recognized as being important in restricting the mobility of contaminants in estuarine environments. To investigate the role of rhizosphere processes in the accumulation of trace metals in mangrove fine nutritive roots, the mangrove sediments and associated fine nutritive roots are collected from five major embayments of Sydney estuary (Australia) for geochemical studies. In this estuary Avicennia marina sediments are accumulating large quantities of trace metals due to presence of abundant fine sediment (fine nutritive roots responds to total sediment chemistry mainly due to rhizosphere sediment geochemical processes resulting in a strong linear correlation between metal concentrations in fine nutritive roots vs. total and bio-available contents in sediments. Accumulation of trace metals in fine nutritive roots is almost always exceeds rhizosphere total sediment metal concentrations. Copyright © 2013 Elsevier Ltd. All rights reserved.

  13. El modelo turístico de la Marina Alta: apuntes para una nueva etapa desde la sostenibilidad como referencia

    Directory of Open Access Journals (Sweden)

    J. Fernando Vera Rebollo

    2000-01-01

    Full Text Available El propósito de las siguientes reflexiones no es otro que aportar algunas ideas en el marco de un debate necesario sobre el modelo territorial-turístico de la Marina Alta, en el contexto de una nueva filosofía de desarrollo que lleva implícitos, como contenidos clave, la gestión racional de los recursos naturales y culturales y la calidad de vida de las poblaciones locales. Son aspectos a los que el turismo puede aportar argumentos, en tanto que su futuro como actividad ha de estar basado en la calidad, la gestión ambiental y la diferenciación de los espacios de destino

  14. Distribution and community composition of lichens on mature mangroves (Avicennia marina subsp. australasica (Walp. J.Everett in New Zealand.

    Directory of Open Access Journals (Sweden)

    Christy L Reynolds

    Full Text Available Mangrove forests of a single trees species, Avicennia marina subsp. australasica are widespread in the upper North Island of New Zealand, but there is little available information on the diversity of epiphytes such as lichens within them. A survey of 200 trees from 20 mangrove sites recorded a total of 106 lichen species from 45 genera. Two of these species are considered to be 'Threatened', five 'At Risk' and 27 'Data Deficient'. Multiple regression indicated that tree diameter (DBH and mean annual rain days positively influenced site species richness. Multidimensional scaling showed that sites from the same geographical region generally formed distinct clusters. Redundancy analysis indicated that mean annual wet days, latitude and DBH measurably influenced species composition.

  15. Temporal and spatial dynamics of ephemeral macroalgal communities in eelgrass, Zostera marina, beds

    DEFF Research Database (Denmark)

    Rasmussen, Jonas; Pedersen, Morten Foldager; Olesen, Birgit

    permanent plots (1 m2) randomly positioned in a 2500 m2 study area during three 8-12 days periods of the growth season. Results show that the algal assemblages were highly dynamic on the small spatial scale as cover within individual plots changed regularly between subsequent observations. The change...... in algal cover was inversely correlated with the cover of eelgrass, Zostera marina, suggesting that algae were retained by the eelgrass leaves. At the larger spatial scale algal cover was less variable and significant changes occurred just a few times during the study periods. Variability was caused either......In a field study we investigated the temporal dynamics of drift-algal assemblages on both small (1 m2) and larger (2500 m2) spatial scales in two shallow (1-3 m) and relatively sheltered locations in Aarhus Bay and Isefjorden, Denmark. Drift-algal cover was estimated every second day in 40...

  16. FACILITIES PLANNING WORKSHOP FOR BLASTING SUPPORT THE ACTIVITY OF DEVELOPMENT AND REPAIR SHIP IN PT. JASA MARINA INDAH UNIT II

    Directory of Open Access Journals (Sweden)

    Samuel Samuel

    2012-07-01

    Full Text Available Blasting in the process of planning the workshop production of new building and ship repair to play a role in providing blasting and paint on the block that will be of erection. As a result of blasting workshop facilities that do not have resulted in low production capacity that can be achieved by this workshop, namely three block ships per month. Capacity blasting and paint shop in this low resulted in low productivity process stage (stage the previous workshops which of course result in a decrease in vessel productivity in general.                 In penelitiaan aims to plan for blasting and paint shop facility which has been adjusted to the planned production capacity of PT. JASA MARINA INDAH II units.                 In this study it - thing to note is to understand the data - the data field for research conducted in terms of both technical and economic terms, with the blasting and paint shop facilities on the construction or repair of ships that have been planned, then the effectiveness of the work and production flow at. Jasa Marina Indah II units can be known.                 Based on the analysis and calculation of both technical and economical it can be identified by the workshop on the process of blasting Blasting efficiency is obtained for 2.55 hours, at 10.16 hours during the painting process, while economical in terms of labor costs can be reduced blasting cost is Rp.930000    for          paint       and         Rp.1.23million

  17. Evidence for strain-specific exometabolomic responses of the coccolithophore Emiliania huxleyi to grazing by the dinoflagellate Oxyrrhis marina.

    Directory of Open Access Journals (Sweden)

    Kelsey L Poulson-Ellestad

    2016-01-01

    Full Text Available The coccolithophore Emiliania huxleyi forms massive blooms and plays a critical role in global elemental cycles, sequestering significant amounts of atmospheric carbon dioxide on geological time scales via production of calcium carbonate coccoliths and emitting dimethyl sulfoniopropionate (DMSP which has the potential for increasing atmospheric albedo. Because grazing in pelagic systems is a major top-down force structuring microbial communities, the influence of grazers on E. huxleyi populations has been of interest to researchers. Roles of DMSP (and related metabolites in interactions between E. huxleyi and protist grazers have been investigated, however, little is known about the release of other metabolites that may influence, or be influenced by, such grazing interactions. We used high-resolution mass spectrometry in an untargeted approach to survey the suite of low molecular weight compounds released by four different E. huxleyi strains in response to grazing by the dinoflagellate Oxyrrhis marina. Overall, a strikingly small number of metabolites were detected from E. huxleyi and O. marina cells, but these were distinctly informative to construct metabolic footprints. At most, E. huxleyi strains shared 25% of released metabolites. Furthermore, there appeared to be no unified metabolic response in E. huxleyi strains to grazing; rather these responses were strain specific. Concentrations of several metabolites also positively correlated with grazer activities, including grazing, ingestion, and growth rates; however, no single metabolite responded uniformly across all strains of E. huxleyi tested. Regardless, grazing clearly transformed the constituents of dissolved organic matter produced by these marine microbes. This study addresses several technical challenges, and presents a platform to further study the influence of chemical cues in aquatic systems and demonstrates the impact of strain diversity and grazing on the complexity of

  18. Patterns of Genetic Variability in Island Populations of the Cane Toad (Rhinella marina from the Mouth of the Amazon.

    Directory of Open Access Journals (Sweden)

    Adam Rick Bessa-Silva

    Full Text Available The Amazonian coast has several unique geological characteristics resulting from the interaction between drainage pattern of the Amazon River and the Atlantic Ocean. It is one of the most extensive and sedimentologically dynamic regions of the world, with a large number of continental islands mostly formed less than 10,000 years ago. The natural distribution of the cane toad (Rhinella marina, one of the world's most successful invasive species, in this complex Amazonian system provides an intriguing model for the investigation of the effects of isolation or the combined effects of isolation and habitat dynamic changes on patterns of genetic variability and population differentiation. We used nine fast-evolving microsatellite loci to contrast patterns of genetic variability in six coastal (three mainlands and three islands populations of the cane toad near the mouth of the Amazon River. Results from Bayesian multilocus clustering approach and Discriminant Analyses of Principal Component were congruent in showing that each island population was genetically differentiated from the mainland populations. All FST values obtained from all pairwise comparisons were significant, ranging from 0.048 to 0.186. Estimates of both recent and historical gene flow were not significantly different from zero across all population pairs, except the two mainland populations inhabiting continuous habitats. Patterns of population differentiation, with a high level of population substructure and absence/restricted gene flow, suggested that island populations of R. marina are likely isolated since the Holocene sea-level rise. However, considering the similar levels of genetic variability found in both island and mainland populations, it is reliable to assume that they were also isolated for longer periods. Given the genetic uniqueness of each cane toad population, together with the high natural vulnerability of the coastal regions and intense human pressures, we suggest

  19. Population genetic structure of eelgrass (Zostera marina on the Korean coast: Current status and conservation implications for future management.

    Directory of Open Access Journals (Sweden)

    Jae Hwan Kim

    Full Text Available Seagrasses provide numerous ecosystem services for coastal and estuarine environments, such as nursery functions, erosion protection, pollution filtration, and carbon sequestration. Zostera marina (common name "eelgrass" is one of the seagrass bed-forming species distributed widely in the northern hemisphere, including the Korean Peninsula. Recently, however, there has been a drastic decline in the population size of Z. marina worldwide, including Korea. We examined the current population genetic status of this species on the southern coast of Korea by estimating the levels of genetic diversity and genetic structure of 10 geographic populations using eight nuclear microsatellite markers. The level of genetic diversity was found to be significantly lower for populations on Jeju Island [mean allelic richness (AR = 1.92, clonal diversity (R = 0.51], which is located approximately 155 km off the southernmost region of the Korean Peninsula, than for those in the South Sea (mean AR = 2.69, R = 0.82, which is on the southern coast of the mainland. South Korean eelgrass populations were substantially genetically divergent from one another (FST = 0.061-0.573, suggesting that limited contemporary gene flow has been taking place among populations. We also found weak but detectable temporal variation in genetic structure within a site over 10 years. In additional depth comparisons, statistically significant genetic differentiation was observed between shallow (or middle and deep zones in two of three sites tested. Depleted genetic diversity, small effective population sizes (Ne and limited connectivity for populations on Jeju Island indicate that these populations may be vulnerable to local extinction under changing environmental conditions, especially given that Jeju Island is one of the fastest warming regions around the world. Overall, our work will inform conservation and restoration efforts, including transplantation for eelgrass populations at the

  20. Consumers' risk elimination in fuel supply chain. The Attica's Marinas yacht fuelling case study

    Energy Technology Data Exchange (ETDEWEB)

    Liapis, Nikolaos [ELINOIL-HELLENIC PETROLEUM COMPANY, S.A., Kifisia (Greece); Zannikos, Fanourios [National Technical Univ. of Athens (Greece). Lab. of Fuels Technology and Lubricants

    2013-06-01

    Oil products distribution system is a complicated supply chain and includes a lot of uncertainties. A limited but quite interesting part of this system is yachting supply in marinas. Yachts usually are equipped with high efficiency and big horsepower modem engines; sea travelling is risky itself so fuel quality contributes significant in safety; many of the yachts are chartered for hired cruises and so operators have the right to purchase tax-free marine diesel, which creates conditions for smuggling and adulteration. All above create a great need for quality control in these fuels, as Greek oil companies do not apply at the moment Total Quality Management (TQM) for securing both quality specifications and quantity paid by the end user. In this research project the Laboratory of Fuel Technology and Lubricants of NTUA collected samples and analysed fuels delivered by tank trucks to yachts in six marinas in the district of Attica. These fuels were marketed by ELINOIL SA, a medium size oil distribution company, operating in Greece. ELINOIL has a market share of 6% of the Greek fuel market but is a market leader in yachting supply in Attica, having more than 50% of the specific market. Problems concerning fuel quality were analysed, direct or indirect causes were tracked, recorded and analysed, always in comparison with data from other Quality Control procedures applied from the company. A specially developed database was updated with facts and figures from incidents and quality issues covering this activity of the company during this period. The project, as it was targeted, detected the quality problems of the specific supply chain and ended to suggestions concerning the building of Quality Assurance System in yachting fuel supply. (orig.)

  1. The MARINA Risk Assessment Strategy: A Flexible Strategy for Efficient Information Collection and Risk Assessment of Nanomaterials.

    Science.gov (United States)

    Bos, Peter M J; Gottardo, Stefania; Scott-Fordsmand, Janeck J; van Tongeren, Martie; Semenzin, Elena; Fernandes, Teresa F; Hristozov, Danail; Hund-Rinke, Kerstin; Hunt, Neil; Irfan, Muhammad-Adeel; Landsiedel, Robert; Peijnenburg, Willie J G M; Sánchez Jiménez, Araceli; van Kesteren, Petra C E; Oomen, Agnes G

    2015-11-27

    An engineered nanomaterial (ENM) may actually consist of a population of primary particles, aggregates and agglomerates of various sizes. Furthermore, their physico-chemical characteristics may change during the various life-cycle stages. It will probably not be feasible to test all varieties of all ENMs for possible health and environmental risks. There is therefore a need to further develop the approaches for risk assessment of ENMs. Within the EU FP7 project Managing Risks of Nanoparticles (MARINA) a two-phase risk assessment strategy has been developed. In Phase 1 (Problem framing) a base set of information is considered, relevant exposure scenarios (RESs) are identified and the scope for Phase 2 (Risk assessment) is established. The relevance of an RES is indicated by information on exposure, fate/kinetics and/or hazard; these three domains are included as separate pillars that contain specific tools. Phase 2 consists of an iterative process of risk characterization, identification of data needs and integrated collection and evaluation of data on the three domains, until sufficient information is obtained to conclude on possible risks in a RES. Only data are generated that are considered to be needed for the purpose of risk assessment. A fourth pillar, risk characterization, is defined and it contains risk assessment tools. This strategy describes a flexible and efficient approach for data collection and risk assessment which is essential to ensure safety of ENMs. Further developments are needed to provide guidance and make the MARINA Risk Assessment Strategy operational. Case studies will be needed to refine the strategy.

  2. The MARINA Risk Assessment Strategy: A Flexible Strategy for Efficient Information Collection and Risk Assessment of Nanomaterials

    Directory of Open Access Journals (Sweden)

    Peter M. J. Bos

    2015-11-01

    Full Text Available An engineered nanomaterial (ENM may actually consist of a population of primary particles, aggregates and agglomerates of various sizes. Furthermore, their physico-chemical characteristics may change during the various life-cycle stages. It will probably not be feasible to test all varieties of all ENMs for possible health and environmental risks. There is therefore a need to further develop the approaches for risk assessment of ENMs. Within the EU FP7 project Managing Risks of Nanoparticles (MARINA a two-phase risk assessment strategy has been developed. In Phase 1 (Problem framing a base set of information is considered, relevant exposure scenarios (RESs are identified and the scope for Phase 2 (Risk assessment is established. The relevance of an RES is indicated by information on exposure, fate/kinetics and/or hazard; these three domains are included as separate pillars that contain specific tools. Phase 2 consists of an iterative process of risk characterization, identification of data needs and integrated collection and evaluation of data on the three domains, until sufficient information is obtained to conclude on possible risks in a RES. Only data are generated that are considered to be needed for the purpose of risk assessment. A fourth pillar, risk characterization, is defined and it contains risk assessment tools. This strategy describes a flexible and efficient approach for data collection and risk assessment which is essential to ensure safety of ENMs. Further developments are needed to provide guidance and make the MARINA Risk Assessment Strategy operational. Case studies will be needed to refine the strategy.

  3. Effects of Hydrogen Sulfide (H2S) on Z. marina seedlings, seed germination and shoot density from 2013-01-16 to 2015-09-11 (NCEI Accession 0156588)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Multiple experiments were conducted to determine the effects hydrogen sulfide had on seedlings and seed germination in the seagrass Zostera marina. One study...

  4. The experimental studies of influence of hydrogen sulfide on species of eelgrass (Zostera japonica and Zostera marina) in Padilla Bay, coastal waters of southeast Alaska conducted from 2013-06-01 to 2013-09-30 (NCEI Accession 0137907)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Two species of eelgrass can be found in Padilla Bay, Washington (Zostera japonica and Zostera marina) and act as a bioindicators of ecosystem health. Many factors...

  5. Has Valkea Marina succeeded in its marketing communications? : Case study examining has a Finnish real estate company reached its clients through its marketing communications and should the company change its marketing communications for the future.

    OpenAIRE

    Räsänen, Roosa

    2012-01-01

    The purpose of this thesis was to examine a Finnish/Spanish real estate agency Valkea Marina’s marketing communications. The research was designed to identify the type of marketing communications Valkea Marina utilises, to discover whether those marketing communications strategies have been profitable, and to analyse ways Valkea Marina can improve its marketing communications in order to increase profits. The research was conducted in two dimensions: secondary research and primary researc...

  6. Nitrosomonas Nm143-like ammonia oxidizers and Nitrospira marina -like nitrite oxidizers dominate the nitrifier community in a marine aquaculture biofilm

    DEFF Research Database (Denmark)

    Foesel, Bärbel U.; Gieseke, Armin; Schwermer, Carsten

    2008-01-01

    with a recirculating marine aquaculture system are presented. Repeated rounds of the full-cycle rRNA approach were necessary to optimize DNA extraction and the probe set for FISH to obtain a reliable and comprehensive picture of the ammonia-oxidizing community. Analysis of the ammonia monooxygenase gene (amo......A) confirmed the results. The most abundant ammonia-oxidizing bacteria (AOB) were members of the Nitrosomonas sp. Nm143-lineage (6.7% of the bacterial biovolume), followed by Nitrosomonas marina-like AOB (2.2% of the bacterial biovolume). Both were outnumbered by nitrite-oxidizing bacteria of the Nitrospira...... marina lineage (15.7% of the bacterial biovolume). Although more than eight other nitrifying populations were detected, including Crenarchaeota closely related to the ammonia-oxidizer ‘Nitrosopumilus maritimus’, their collective abundance was below 1% of the total biofilm volume; their contribution...

  7. Vegetación marina en la elaboración de bloques multinutritivos para la alimentación de rumiantes

    Directory of Open Access Journals (Sweden)

    Arturo F. Castellanos Ruelas

    2010-01-01

    así como sus interacciones. Los resultados indicaron que la vegetación marina tuvo un elevado contenido en proteína y en materia mineral. Al incrementar la melaza en la formulación arriba del 35 % ó el CaOH más allá del 5 %, se redujo la carga máxima de los BM (P<0.01. Lo mismo sucedió al incrementar la urea arriba del 6 % (P<0.05. Se concluye que es factible el empleo de vegetación marina como fuente de proteína y minerales en la elaboración de BM, logrando materiales con buena dureza.

  8. Characterisation of environmental forcing on Zostera marina L. plastochrone interval dynamics in the Punta Banda Estuary, B.C. Mexico: an empirical modelling approach

    Directory of Open Access Journals (Sweden)

    Elena Solana-Arellano

    2009-03-01

    Full Text Available This paper presents a characterisation of the most significant environmental influences on plastochrone interval variation for Zostera marina L. in Punta Banda estuary B.C. Mexico. Data were collected from April 1998 to December 2001. Using correlation and principal component analysis, we found that the combination of sea surface temperature, light radiation and dissolved nutrients explains the observed variability consistently. Sea surface temperature was found to be the dominant environmental influence (r=0.89, p

  9. Modelling the growth kinetics of Kocuria marina DAGII as a function of single and binary substrate during batch production of β-Cryptoxanthin.

    Science.gov (United States)

    Mitra, Ruchira; Chaudhuri, Surabhi; Dutta, Debjani

    2017-01-01

    In the present investigation, growth kinetics of Kocuria marina DAGII during batch production of β-Cryptoxanthin (β-CRX) was studied by considering the effect of glucose and maltose as a single and binary substrate. The importance of mixed substrate over single substrate has been emphasised in the present study. Different mathematical models namely, the Logistic model for cell growth, the Logistic mass balance equation for substrate consumption and the Luedeking-Piret model for β-CRX production were successfully implemented. Model-based analyses for the single substrate experiments suggested that the concentrations of glucose and maltose higher than 7.5 and 10.0 g/L, respectively, inhibited the growth and β-CRX production by K. marina DAGII. The Han and Levenspiel model and the Luong product inhibition model accurately described the cell growth in glucose and maltose substrate systems with a R (2) value of 0.9989 and 0.9998, respectively. The effect of glucose and maltose as binary substrate was further investigated. The binary substrate kinetics was well described using the sum-kinetics with interaction parameters model. The results of production kinetics revealed that the presence of binary substrate in the cultivation medium increased the biomass and β-CRX yield significantly. This study is a first time detailed investigation on kinetic behaviours of K. marina DAGII during β-CRX production. The parameters obtained in the study might be helpful for developing strategies for commercial production of β-CRX by K. marina DAGII.

  10. Metabolic engineering of the Chl d-dominated cyanobacterium Acaryochloris marina: production of a novel Chl species by the introduction of the chlorophyllide a oxygenase gene.

    Science.gov (United States)

    Tsuchiya, Tohru; Mizoguchi, Tadashi; Akimoto, Seiji; Tomo, Tatsuya; Tamiaki, Hitoshi; Mimuro, Mamoru

    2012-03-01

    In oxygenic photosynthetic organisms, the properties of photosynthetic reaction systems primarily depend on the Chl species used. Acquisition of new Chl species with unique optical properties may have enabled photosynthetic organisms to adapt to various light environments. The artificial production of a new Chl species in an existing photosynthetic organism by metabolic engineering provides a model system to investigate how an organism responds to a newly acquired pigment. In the current study, we established a transformation system for a Chl d-dominated cyanobacterium, Acaryochloris marina, for the first time. The expression vector (constructed from a broad-host-range plasmid) was introduced into A. marina by conjugal gene transfer. The introduction of a gene for chlorophyllide a oxygenase, which is responsible for Chl b biosynthesis, into A. marina resulted in a transformant that synthesized a novel Chl species instead of Chl b. The content of the novel Chl in the transformant was approximately 10% of the total Chl, but the level of Chl a, another Chl in A. marina, did not change. The chemical structure of the novel Chl was determined to be [7-formyl]-Chl d(P) by mass spectrometry and nuclear magnetic resonance spectroscopy. [7-Formyl]-Chl d(P) is hypothesized to be produced by the combined action of chlorophyllide a oxygenase and enzyme(s) involved in Chl d biosynthesis. These results demonstrate the flexibility of the Chl biosynthetic pathway for the production of novel Chl species, indicating that a new organism with a novel Chl might be discovered in the future.

  11. Universidad y ciencias del mar: a treinta años del Instituto Interuniversitario de Biología Marina de Mar del Plata

    OpenAIRE

    Olivier, S.R.

    1990-01-01

    Hace treinta años se creaba el Instituto Interuniversitario de Biología Marina de Mar del Plata (IIBM), Argentina, posteriormente desaparecido por influjo de las vicisitudes políticas acontecidas desde entonces. Uno de sus fundadores analiza los principios institucionales y científicos que guiaron el accionar de aquella entidad, a la vez que reflexiona acerca de la situación actual de las ciencias del mar.

  12. Turismo versus pesca artesanal. A propósito de la reserva marina de la isla de La Graciosa y los islotes del norte de Lanzarote

    Directory of Open Access Journals (Sweden)

    Gloria Cabrera Socorro

    2004-01-01

    Full Text Available La Reserva Marina de La Graciosa y los Islotes del Norte de Lanzarote (RMLGINL se creó en 1995 y, desde entonces, las actividades pesqueras de la población local y su cultura del mar han ido decreciendo progresivamente mientras que las actividades turísticas siguen emergiendo como la panacea en una pequeña isla, como La Graciosa, con no más de 600 habitantes. En este artículo tratamos de analizar cómo estos dos procesos están relacionados y qué papel está jugando la reserva marina en este caso. Usamos no sólo información cualitativa de los pescadores locales y de sus grupos domésticos, sino también cuantitativa contrastando algunos parámetros concretos como el número de pescadores y los barcos de pesca activos, los negocios relacionados con el sector servicios (restaurantes, apartamentos, etc., antes y después de implementación de la reserva marina. En este sentido, un importante elemento es la percepción de esta institución desde la población local, y la evolución de su actitud a favor o en contra. Estos procesos están relacionados con el diseño institucional de las reservas marinas en las Islas Canarias, en donde la participación de las poblaciones pesqueras locales está severamente limitada.

  13. An Analysis Of Employee Fraud And Effectiveness Of Applied Management Strategies To Prevent Fraud At PT. Bank Sulutgo Marina Plaza Branch Manado

    OpenAIRE

    Djamaluddin, Olivia Mashita

    2016-01-01

    Employee fraud has been acknowledge as one of the principal threat to the development of banking sectors worldwide. No company is immune to fraud. This research analysed fraud risk factors and any applied fraud prevention management strategies at PT. Bank Sulut Marina Plaza Manado. A qualitative method was applied in this research. Data was collected by means of purposive sampling method through interview of ten selected respondents. This research revealed that factors of pressure, opportunit...

  14. Comparación ontogénica de la frecuencia de muda en Rhinella marina (Anura, Bufonidae Ontogenetic comparison of the molting frequency in Rhinella marina (Anura: Bufonidae

    Directory of Open Access Journals (Sweden)

    Teófila M. Triana

    2013-03-01

    Full Text Available La muda es un proceso de renovación de la capa externa de la epidermis (estrato corneo de los anfibios, la cual suministra protección contra daños, patógenos y pérdida de agua. Este trabajo evalúa la frecuencia de muda entre juveniles y adultos de Rhinella marina (Linnaeus, 1758 y la tasa de ocurrencia entre el día y la noche. Para esto, se realizaron dos observaciones diarias (7 am y 7 pm, entre Octubre de 2011 y Marzo de 2012, a tres grupos de individuos clasificados según su tamaño longitud rostro-cloaca, como adultos (promedio=80 mm, juveniles medianos (promedio=19 mm y juveniles pequeños (promedio=13 mm. Estos animales fueron colocados en terrarios en el laboratorio y marcados en el dorso a través de un punto hecho con un corrector de tinta. La muda se determinó por la pérdida total de la marca y una coloración brillante en el dorso. Se encontró una diferencia significativa (Kruskal-Wallis, H=19.84, pMolting is a process of constant renewal of the outer layer of epidermis (stratum corneum in amphibians, which provides a barrier against injury, pathogens and evaporative water loss. This paper evaluates the molting frequency between juveniles and adults of Rhinella marina (Linnaeus, 1758 and between the night and day time. Two daily observations, at 7 am and 7 pm, were made between October 2011 and March 2012 to three groups of individuals, classified by their size, snout vent length, as adults (mean=80 mm, medium juveniles (mean=19 mm and small juveniles (mean=13 mm. The animals were placed in terrariums in the laboratory and marked on their back with a spot of correction fluid. The molting was determined by the total loss of the mark and a bright color on the dorsal skin. We found a significant difference (Kruskal-Wallis, H=19.84, p<0.0001 in the molting frequency among the three groups: adults=7.5 days, medium juveniles=5.4 days, and small juveniles=5.3 days. Also, between the number of molting frogs during the night and day

  15. Marinas may act as hubs for the spread of the pseudo-indigenous bryozoan Amathia verticillata (Delle Chiaje, 1822 and its associates

    Directory of Open Access Journals (Sweden)

    Agnese Marchini

    2015-09-01

    Full Text Available The spaghetti bryozoan Amathia verticillata, formerly known as Zoobotryon verticillatum, was first described in 1822 from Naples, Italy, although this species was already present in 1807 at Cadiz, Spain. This ctenostome has long been considered a native species in the Mediterranean Sea but it has recently been suggested to be of Caribbean origin. It is most likely to have been introduced by vessels as hull fouling. This pseudo-indigenous species, i.e. a non-indigenous species (NIS having been perceived to be native, has been found in several marinas and harbours within the Mediterranean Sea. In November 2014, this bryozoan species was abundant in the La Grande Motte marina on the south coast of France. Several thousand colonies were estimated to be present within this marina attached to the floating pontoon units that supported a floating boardwalk. Of the berthed craft examined, 31% were fouled with this species, and it was occasionally a prominent fouling species. Several macroinvertebrate species were associated with A. verticillata colonies, including some NIS, Paracerceis sculpta, Paranthura japonica and Caprella scaura, that are recorded for the first time from the Mediterranean coast of France. A. verticillata might support their transfer elsewhere by providing a habitat and substrate when attached to vessel hulls.

  16. COVE, MARINA, and the Future of On-Board Processing (OBP) Platforms for CubeSat Science Missions

    Science.gov (United States)

    Pingree, P.; Bekker, D. L.; Bryk, M.; DeLucca, J.; Franklin, B.; Hancock, B.; Klesh, A. T.; Meehan, C.; Meshkaty, N.; Nichols, J.; Peay, C.; Rider, D. M.; Werne, T.; Wu, Y.

    2012-12-01

    The CubeSat On-board processing Validation Experiment (COVE), JPL's first CubeSat payload launched on October 28, 2011, features the Xilinx Virtex-5QV Single event Immune Reconfigurable FPGA (SIRF). The technology demonstration mission was to validate the SIRF device running an on-board processing (OBP) algorithm developed to reduce the data set by 2-orders of magnitude for the Multi-angle SpectroPolarimetric Imager (MSPI), an instrument under development at JPL (PI: D. Diner). COVE has a single data interface to the CubeSat flight computer that is used to transfer a static image taken from the CubeSat camera and store it to local memory where the FPGA then reads it to run the algorithm on it. In the next generation COVE design, called MARINA, developed for the GRIFEX CubeSat project, the OBP board is extended, using rigid-flex PCB technology, to provide an interface to a JPL-developed Read-Out Integrated Circuit (ROIC) hybridized to a detector developed by Raytheon. In this configuration the focal plane array (FPA) data can be streamed directly to the FPGA for data processing or for storage to local memory. The MARINA rigid-flex PCB design is integrated with a commercial camera lens to create a 1U instrument payload for integration with a CubeSat under development by the University of Michigan and planned for launch in 2014. In the GRIFEX technology demonstration, the limited on-board storage capacity is filled by high-rate FPA data in less than a second. The system is also limited by the CubeSat downlink data rate and several ground station passes are required to transmit this limited amount of data. While this system is sufficient to validate the ROIC technology on-orbit, the system cannot be operated in a way to perform continuous science observations due to the on-board storage and data downlink constraints. In order to advance the current platform to support sustained science observations, more on-board storage is needed. Radiation tolerant memory

  17. La corrosión en atmósferas marinas. Efecto de la distancia a la costa

    Directory of Open Access Journals (Sweden)

    Chico, B.

    1998-05-01

    Full Text Available In marine atmospheres the deposition of saline particles on the surface of metals intensifies the metallic corrosion process. However, quantitative information about the effect of atmospheric salinity on metallic corrosion is very scarce. This paper reports the relationship between salinity and metallic corrosion, where a clear linear relation (r = 0.97 has been found for a broad interval of salinities (4-500 mg Cl-/m2d, as well as the relationship between salinity (or metallic corrosion and distance from the coast. A hyperbolic function seems to be established between both variables; there is an exponential drop in salinity (or corrosion as shoreline distance increases tending towards an asymptotic value. The study has been based on information obtained from field research conducted at a marine atmosphere in Tarragona (Spain and data compiled from the literature.

    En las atmósferas marinas, el depósito de partículas salinas sobre la superficie de los metales, acelera el proceso de corrosión metálica. Sin embargo, es muy escasa la información cuantitativa acerca del efecto de la salinidad atmosférica sobre la corrosión metálica. Este trabajo presenta la relación existente entre salinidad y corrosión metálica, encontrándose una clara relación lineal (r = 0,97 para un amplio intervalo de salinidades (4-500 mg Cl-/m2d, así como la relación existente entre salinidad (o corrosión metálica y distancia a la costa. Aquí, parece establecerse una función hiperbólica entre ambas variables, existiendo un decrecimiento exponencial de la salinidad (o corrosión a medida que aumenta la distancia a la costa, tendiendo hacia un valor asintótico. Este estudio analiza la información obtenida en una zona de experimentación ubicada en una atmósfera marina en Tarragona (España junto con datos recopilados de la literatura.

  18. The Importance of Zostera marina to a Local Food Web Based on the Analysis of Compound Specific Isotopes in Maquoit Bay, Gulf of Maine

    Science.gov (United States)

    Doolittle, H. A.; Johnson, B. J.; Ambrose, W. G.; Locke, W.; Harris, C. M.

    2010-12-01

    Zostera marina (also known as eelgrass) is an important primary producer in near shore ecosystems in the Gulf of Maine, providing both habitat and nutrients for a variety of organisms (e.g., crustaceans, polychaetes, gastropods, and fish). The purpose of this study is to use compound specific δ13C analyses of essential amino acids to determine the degree to which organic matter derived from isotopically distinct primary producers (e.g., eelgrass, phytoplankton, and epiphytic algae) contribute to the diets of snails, shrimp, and fish in an eelgrass system in Casco Bay. Maquoit Bay, located in northwestern Casco Bay, in the Gulf of Maine, is a shallow estuarine system that is characterized by silt and clay sized sediments and the presence of extensive eelgrass beds. Amino acid concentrations and δ13C compositions were determined for a variety of sample-types collected in July-August, 2010, from three sites in the study area, including muscle tissue from Tautogolabrus adspersus (cunner), Gasterosteus aculeatus (3-spined stickleback), Nassarius obsoletus (snail), and Mysis spp. (shrimp), seston (i.e., phytoplankton), Z. marina, and epiphytic algae. TFAA amino acid derivatives of the total hydrolyzate were analyzed by GC-FID for amino acid concentration, and by GC-c-IRMS- for carbon isotope composition. Muscle tissue was dominated by glutamic and aspartic acids, and leucine, whereas Zostera marina was dominated by aspartic and glutamic acids, and proline. Phenylalanine and leucine in Z. marina are approximately 10 ‰ enriched in 13C relative to these same amino acids in the seston. The carbon isotope values of these essential amino acids are significantly more enriched in 13C for N. obsoletus than for T. adspersus, G. aculeatus, and Mysis spp. These data suggest that organic matter derived from Z. marina and/or epiphytic algae is more important in the diets of N. obsoletus, and organic matter derived from seston is more important for the diets of T. adspersus, G

  19. Monti lubab Itaalia kasvule viia / Hendrik Vosman

    Index Scriptorium Estoniae

    Vosman, Hendrik

    2011-01-01

    Itaalias moodustustati uus valitsus, kuhu ei kuulu ühtegi poliitikut. Vihased meeleavaldused Itaalias näitasid, et rahvas ei pruugi uue peaministri Mario Monti reformide läbisurumist rahumeelselt võtta. Monti valitsuse ettepanekud Itaalia majandusreformideks

  20. Lanfrediella amphicirrus gen. nov. sp. nov. Nematotaeniidae (Cestoda: Cyclophyllidea), a tapeworm parasite of Rhinella marina (Linnaeus, 1758) (Amphibia: Bufonidae).

    Science.gov (United States)

    Melo, Francisco Tiago de Vasconcelos; Giese, Elane Guerreiro; Furtado, Adriano Penha; Soares, Maurílio José; Gonçalves, Evonnildo Costa; Vallinoto, Antonio Carlos Rosário; Santos, Jeannie Nascimento dos

    2011-09-01

    The family Nematotaeniidae, tapeworms commonly found in the small intestines of amphibians and reptiles, includes 27 recognised species distributed among four genera: Bitegmen Jones, Cylindrotaenia Jewell, Distoichometra Dickey and Nematotaenia Lühe. The taxonomy of these cestodes is poorly defined, due in part to the difficulties of observing many anatomical traits. This study presents and describes a new genus and species of nematotaeniid parasite found in cane toads (Rhinella marina) from eastern Brazilian Amazonia. The cestodes were collected during the necropsy of 20 hosts captured in the urban area of Belém, Pará. The specimens were fixed and processed for light microscopy, scanning electron microscopy (SEM) and three-dimensional (3D) reconstruction. Samples were also collected for molecular analyses. The specimens presented a cylindrical body, two testes and paruterine organs. However, they could not be allocated to any of the four existing nematotaeniid genera due to the presence of two each of dorsal compact medullary testes, cirri, cirrus pouches, genital pores, ovaries and vitelline glands per mature segment. Lanfrediella amphicirrus gen. nov. sp. nov. is the first nematotaeniid studied using Historesin analysis, SEM and 3D reconstruction, and it is the second taxon for which molecular data have been deposited in GenBank.

  1. Identification of rosmarinic acid and sulfated flavonoids as inhibitors of microfouling on the surface of eelgrass Zostera marina.

    Science.gov (United States)

    Guan, Chi; Parrot, Delphine; Wiese, Jutta; Sönnichsen, Frank D; Saha, Mahasweta; Tasdemir, Deniz; Weinberger, Florian

    2017-11-01

    A bioassay-guided approach was used to identify defense compounds that are present on the surface of Zostera marina and which inhibit settlement of microfoulers at natural concentrations. Moderately polar eelgrass surface extracts inhibited the settlement of seven marine bacteria and one yeast that originated from non-living substrata. In contrast, five other bacterial strains that had been directly isolated from eelgrass surfaces were all insensitive, which suggested a selective effect of surface metabolites on the microbial communities present on eelgrass. Bioassay-guided isolation of active compounds from the extracts in combination with UPLC-MS and 1H-NMR spectroscopy resulted in the identification of rosmarinic acid, luteolin-7-sulfate and diosmetin-7-sulfate or its isomer chrysoeriol-7-sulfate. All three compounds are nontoxic repellents, as they did not inhibit bacterial growth, but prevented bacterial settlement in a dose-dependent manner. Between 15.6 and 106.8 μg ml-1 of rosmarinic acid were present on the eelgrass surface, enough for half maximal settlement inhibition of bacteria.

  2. [Characteristics of adhesion of epiphytic bacteria on leaves of the seagrass Zostera marina and on abiotic surfaces].

    Science.gov (United States)

    Kurilenko, V V; Ivanova, E P; Mikhaĭlov, V V

    2007-01-01

    A comparative study of the adhesion of epiphytic bacteria and marine free-living, saprophytic, and pathogenic bacteria on seagrass leaves and abiotic surfaces was performed to prove the occurrence of true epiphytes of Zostera marina and to elucidate the bacterium-plant symbiotrophic relationships. It was shown that in the course of adhesion to the seagrass leaves of two taxonomically different bacteria, Cytophaga sp. KMM 3552 and Pseudoalteromonas citrea KMM 461, isolated from the seagrass surface, the number of viable cells increased 3-7-fold after 60 h of incubation, reaching 1.0-2.0 x 10(5) cells/cm2; however, in the case of adhesion of these bacteria to abiotic surfaces, such as glass or metal, virtually no viable cells were observed after 60 h of incubation. Such selectivity of cell adhesion was not observed in the case of three other bacterial species studied, viz., Vibrio alginolyticus KMM 3551, Bacillus subtilis KMM 430, and Pseudomonas aeruginosa KMM 433. The amount of viable cells of V. alginolyticus KMM 3551 adsorbed on glass and metal surfaces increased twofold after 40 h of incubation. The cells of saprophytic B. subtilis KMM 430 and pathogenic P. aeruginosa KMM 433 adsorbed on three studied substrata remained viable for 36 h and died by the 60th hour of incubation.

  3. Actividad antibacteriana de extractos etanólicos de macroalgas marinas de la costa central del Perú

    Directory of Open Access Journals (Sweden)

    Claudio Magallanes

    2013-06-01

    Full Text Available Se evaluó la actividad antibacteriana de extractos etanólicos de 12 especies de macroalgas marinas. Los extractos fueron obtenidos de acuerdo al protocolo modificado de Vlachos et al. (1996. Cada extracto se enfrentó contra 5 cepas bacterianas de origen clínico y 6 no clínico perteneciente a los géneros Staphylococcus, Enterococcus, Pseudomonas, Escherichia, Salmonella, Aeromonas y Vibrio. De 12 especies de algas ensayadas solamente 5 (Grateloupia doryphora, Ahnfeltiopsis durvillaei, Prionitis decipiens, Petalonia fascia y Bryopsis plumosa presentaron algún efecto antibacteriano. Asimismo, de 11 cepas bacterianas probadas solamente las cepas clínicas Staphylococcus aureus ATCC 25923 y Enterococcus faecalis ATCC 29212 y la cepa no clínica Staphylococcus aureus ATCC 6633 fueron sensibles a los extractos algales. El extracto etanólico de B. plumosa presentó el mayor efecto antibacteriano contra las dos cepas de S. aureus, manifestándose en el mayor tamaño de sus halos de inhibición, mientras que el extracto de P. fascia mostró mayor espectro antibacteriano, inhibiendo a las 3 cepas mencionadas.

  4. Lanfrediella amphicirrus gen. nov. sp. nov. Nematotaeniidae (Cestoda: Cyclophyllidea, a tapeworm parasite of Rhinella marina (Linnaeus, 1758 (Amphibia: Bufonidae

    Directory of Open Access Journals (Sweden)

    Francisco Tiago de Vasconcelos Melo

    2011-09-01

    Full Text Available The family Nematotaeniidae, tapeworms commonly found in the small intestines of amphibians and reptiles, includes 27 recognised species distributed among four genera: Bitegmen Jones, Cylindrotaenia Jewell, Distoichometra Dickey and Nematotaenia Lühe. The taxonomy of these cestodes is poorly defined, due in part to the difficulties of observing many anatomical traits. This study presents and describes a new genus and species of nematotaeniid parasite found in cane toads (Rhinella marina from eastern Brazilian Amazonia. The cestodes were collected during the necropsy of 20 hosts captured in the urban area of Belém, Pará. The specimens were fixed and processed for light microscopy, scanning electron microscopy (SEM and three-dimensional (3D reconstruction. Samples were also collected for molecular analyses. The specimens presented a cylindrical body, two testes and paruterine organs. However, they could not be allocated to any of the four existing nematotaeniid genera due to the presence of two each of dorsal compact medullary testes, cirri, cirrus pouches, genital pores, ovaries and vitelline glands per mature segment. Lanfrediella amphicirrus gen. nov. sp. nov. is the first nematotaeniid studied using Historesin analysis, SEM and 3D reconstruction, and it is the second taxon for which molecular data have been deposited in GenBank.

  5. Experimental weed control of Najas marina ssp. intermedia and Elodea nuttallii in lakes using biodegradable jute matting

    Directory of Open Access Journals (Sweden)

    Markus A. Hoffmann

    2013-10-01

    Full Text Available The use of jute matting in managing the invasive aquatic macrophyte species Elodea nuttallii (Planch. H. St. John and Najas marina ssp. intermedia (Wolfg. ex Gorski Casper (Najas intermedia was studied in laboratory experiments and field trials. Four German lakes with predominant population of Najas intermedia or Elodea nuttalli were chosen for the experiment and areas between 150 and 300 m² were covered with jute textile. The effect of the matting on the growth of invasive and non-invasive macrophytes was determined through comparison with control transects. Biodegradable jute matting successfully suppressed the invasive macrophyte Najas intermedia and significantly reduced the growth of Elodea nuttalli in lakes. The results indicate that the capability of the matting to inhibit the growth of Elodea nuttallii and Najas intermedia depends on the mesh size of the jute weaving and that environmental conditions can affect its efficiency. Various indigenous species like Charales or Potamogeton pusillus L. were able to grow through the jute fabric and populate the treated areas. Until the end of the vegetation period, none of the invasive species were able to penetrate the covering and establish a stable population; in fact, in the subsequent year the jute matting affected only the spread of Najas intermedia. Jute matting proved to be an easy-to-use and cheap method to control the growth of Elodea nuttallii and Najas intermedia.

  6. Living up to its name? The effect of salinity on development, growth, and phenotype of the "marine" toad (Rhinella marina).

    Science.gov (United States)

    Wijethunga, Uditha; Greenlees, Matthew; Shine, Richard

    2016-02-01

    The highly permeable integument of amphibians renders them vulnerable to chemical characteristics of their environment, especially during the aquatic larval stage. As the cane toad (Rhinella marina, Bufonidae) invades southwards along the east coast of Australia, it is encountering waterbodies with highly variable conditions of temperature, pH, and salinity. Understanding the tolerance of toads to these conditions can clarify the likely further spread of the invader, as well as the adaptability of the species to novel environmental challenges. We measured salinity in waterbodies in the field and conducted laboratory trials to investigate the impacts of salinity on toad viability. Eggs and tadpoles from the southern invasion front tolerated the most saline conditions we found in potential spawning ponds during surveys [equivalent to 1200 ppm (3.5 % the salinity of seawater)]. Indeed, high-salinity treatments increased tadpole body sizes, accelerated metamorphosis, and improved locomotor ability of metamorphs (but did not affect metamorph morphology). At very low salinity [40 ppm (0.1 % seawater)], eggs hatched but larvae did not develop past Gosner stage 37. Our study shows that the egg and larval life stages of cane toads can tolerate wide variation in the salinity of natal ponds and that this aspect of waterbody chemistry is likely to facilitate rather than constrain continued southward expansion of the toad invasion front in eastern Australia.

  7. Spergularia marina Induces Glucagon-Like Peptide-1 Secretion in NCI-H716 Cells Through Bile Acid Receptor Activation

    Science.gov (United States)

    Kim, Kyong; Lee, Yu Mi; Rhyu, Mee-Ra

    2014-01-01

    Abstract Spergularia marina Griseb. (SM) is a halophyte that grows in mud flats. The aerial portions of SM have been eaten as vegetables and traditionally used to prevent chronic diseases in Korea. However, there has been no scientific report that demonstrates the pharmacological effects of SM. Glucagon-like peptide-1 (GLP-1) is important for the maintenance of glucose and energy homeostasis through acting as a signal in peripheral and neural systems. To discover a functional food for regulating glucose and energy homeostasis, we evaluated the effect of an aqueous ethanolic extract (AEE) of SM on GLP-1 release from enteroendocrine NCI-H716 cells. In addition, we explored the Takeda G-protein-coupled receptor 5 (TGR5) agonist activity of AEE-SM in Chinese hamster ovary (CHO)-K1 cells transiently transfected with human TGR5. As a result, treatment of NCI-H716 cells with AEE-SM increased GLP-1 secretion and intracellular Ca2+ and cyclic AMP (cAMP) levels in a dose-dependent manner. Transfection of NCI-H716 cells with TGR5-specific small interference RNA inhibited AEE-SM-induced GLP-1 secretion and the increase in Ca2+ and cAMP levels. Moreover, AEE-SM showed that the TGR5 agonist activity in CHO-K1 cells transiently transfected with TGR5. The results suggest that AEE-SM might be a candidate for a functional food to regulate glucose and energy homeostasis. PMID:25260089

  8. An economical non-destructive method for estimating eelgrass, Zostera marina (Potamogetonaceae leaf growth rates: formal development and use in northwestern Baja California

    Directory of Open Access Journals (Sweden)

    Elena Solana-Arellano

    2008-09-01

    Full Text Available Seagrass beds provide much of the primary production in estuaries; host many fishes and fish larvae, and abate erosion. The present study presents original analytical methods for estimating mean leaf-growth rates of eelgrass (Zostera marina. The method was calibrated by using data collected in a Z. marina meadow at Punta Banda estuary in Baja California, Mexico. The analytical assessments were based on measurements of leaf length and standard regression procedures. We present a detailed explanation of the formal procedures involved in the derivation of these analytical methods. The measured daily leaf-growth rate was 10.9 mm d-1 leaf-1. The corresponding value projected by our method was 10.2 mm d-1 leaf-1. The associated standard errors were of 0.53 and 0.56 mm d-1 leaf-1 respectively. The method was validated by projecting leaf-growth rates from an independent data set, which gave consistent results. The use of the method to obtain the mean leaf growth rate of a transplanted plot is also illustrated. Comparison of our leaf-growth data with previously reported assessments show the significant forcing of sea-surface temperature on eelgrass leaf dynamics. The formal constructs provided here are of general scope and can be applied to equivalent eelgrass data sets in a straightforward manner. Rev. Biol. Trop. 56 (3: 1003-1013. Epub 2008 September 30.Las praderas de pastos marinos abaten la erosión y aportan gran parte de la productividad primaria de los esteros y son refugio de muchos peces y sus larvas. El presente trabajo introduce métodos analíticos para estimar las tasas medias de crecimiento foliar de Zostera marina L. y sus varianzas. La calibración del método se llevó a cabo utilizando datos de una pradera de esta fanerógama en el Estero de Punta Banda Baja California, México. Las referidas estimaciones analíticas, se basan en medias de longitud foliar y en procedimientos estandarizados de regresión. Dichas determinaciones son por

  9. Identification of light-harvesting chlorophyll a/b-binding protein genes of Zostera marina L. and their expression under different environmental conditions

    Science.gov (United States)

    Kong, Fanna; Zhou, Yang; Sun, Peipei; Cao, Min; Li, Hong; Mao, Yunxiang

    2016-02-01

    Photosynthesis includes the collection of light and the transfer of solar energy using light-harvesting chlorophyll a/b-binding (LHC) proteins. In high plants, the LHC gene family includes LHCA and LHCB sub-families, which encode proteins constituting the light-harvesting complex of photosystems I and II. Zostera marina L. is a monocotyledonous angiosperm and inhabits submerged marine environments rather than land environments. We characterized the Lhca and Lhcb gene families of Z. marina from the expressed sequence tags (EST) database. In total, 13 unigenes were annotated as ZmLhc, 6 in Lhca family and 7 in ZmLhcb family. ZmLHCA and ZmLHCB contained the conservative LHC motifs and amino acid residues binding chlorophyll. The average similarity among mature ZmLHCA and ZmLHCB was 48.91% and 48.66%, respectively, which indicated a high degree of divergence within ZmLHChc gene family. The reconstructed phylogenetic tree showed that the tree topology and phylogenetic relationship were similar to those reported in other high plants, suggesting that the Lhc genes were highly conservative and the classification of ZmLhc genes was consistent with the evolutionary position of Z. marina. Real-time reverse transcription (RT) PCR analysis showed that different members of ZmLhca and ZmLhcb responded to a stress in different expression patterns. Salinity, temperature, light intensity and light quality may affect the expression of most ZmLhca and ZmLhcb genes. Inorganic carbon concentration and acidity had no obvious effect on ZmLhca and ZmLhcb gene expression, except for ZmLhca6.

  10. Molecular cloning of class III chitinase gene from Avicennia marina and its expression analysis in response to cadmium and lead stress.

    Science.gov (United States)

    Wang, Li-Ying; Wang, You-Shao; Zhang, Jing-Ping; Gu, Ji-Dong

    2015-10-01

    Mangrove species have high tolerance to heavy metal pollution. Chitinases have been widely reported as defense proteins in response to heavy metal stress in terrestrial plants. In this study, a full-length cDNA sequence encoding an acidic and basic class III chitinase (AmCHI III) was cloned by using RT-PCR and RACE methods in Avicennia marina. AmCHI III mRNA expression in leaf of A. marina were investigated under Cd, Pb stresses on using real-time quantitative PCR. The deduced AmCHI III protein consists of 302 amino acids, including a signal putative peptide region, and a catalytic domain. Homology modeling of the catalytic domain revealed a typical molecular structure of class III plant chitinases. Results further demonstrated that the regulation of AmCHI III mRNA expression in leaves was strongly dependent on Cd, Pb stresses. AmCHI III mRNA expressions were significantly increased in response to Cd, Pb, and peaked at 7 days Cd-exposure, 7 days Pb-exposure, respectively. AmCHI III mRNA expression exhibited more sensitive to Pb stress than Cd stress. This work was the first time cloing chitinase from A. marina, and it brought evidence on chitinase gene involving in heavy metals (Cd(2+) and Pb(2+)) resistance or detoxification in plants. Further studies including the promoter and upstream regulation, gene over-expression and the response of mangrove chitinases to other stresses will shed more light on the role of chitinase in mangrove plants.

  11. Soil and Rhizosphere Associated Fungi in Grey Mangroves (Avicennia marina) from the Red Sea - A Metagenomic Approach

    KAUST Repository

    Simoes, Marta

    2015-11-05

    Covering a quarter of the world’s tropical coastlines and being one of the most threatened ecosystems, mangroves are among the major sources of terrestrial organic matter to oceans and harbor a wide microbial diversity. In order to protect, restore, and better understand these ecosystems, researchers have extensively studied their microbiology, yet few surveys have focused on their fungal communities. Our lack of knowledge is even more pronounced for specific fungal populations, such as the ones associated with the rhizosphere. Likewise, the Red Sea grey mangroves (Avicennia marina) remain poorly characterized, and understanding of their fungal communities still relies on cultivation-dependent methods. In this study, we analyzed metagenomic datasets from grey mangrove rhizosphere and bulk soil samples collected in the Red Sea coast, to obtain a snapshot of their fungal communities. Our data indicated that Ascomycota was the dominant phylum (76%–85%), while Basidiomycota was less abundant (14%–24%), yet present in higher numbers than usually reported for such environments. Fungal communities were more stable within the rhizosphere than within the bulk soil, both at class and genus level. This finding is consistent with the intrinsic patchiness in soil sediments and with the selection of specific microbial communities by plant roots. Our study indicates the presence of several species on this mycobiome that were not previously reported as mangrove-associated. In particular, we detected representatives of several commercially-used fungi, e.g., producers of secreted cellulases and anaerobic producers of cellulosomes. These results represent additional insights into the fungal community of the grey mangroves of the Red Sea, and show that they are significantly richer than previously reported.

  12. Desiccation risk drives the spatial ecology of an invasive anuran (Rhinella marina) in the Australian semi-desert.

    Science.gov (United States)

    Tingley, Reid; Shine, Richard

    2011-01-01

    Some invasive species flourish in places that impose challenges very different from those faced in their native geographic ranges. Cane toads (Rhinella marina) are native to tropical and subtropical habitats of South and Central America, but have colonised extremely arid regions over the course of their Australian invasion. We radio-tracked 44 adult cane toads at a semi-arid invasion front to investigate how this invasive anuran has managed to expand its geographic range into arid areas that lie outside of its native climatic niche. As predicted from their low physiological control over rates of evaporative water loss, toads selected diurnal shelter sites that were consistently cooler and damper (and thus, conferred lower water loss rates) than nearby random sites. Desiccation risk also had a profound influence on rates of daily movement. Under wet conditions, toads that were far from water moved further between shelter sites than did conspecifics that remained close to water, presumably in an attempt to reach permanent water sources. However, this relationship was reversed under dry conditions, such that only toads that were close to permanent water bodies made substantial daily movements. Toads that were far from water bodies also travelled along straighter paths than did conspecifics that generally remained close to water. Thus, behavioural flexibility--in particular, an ability to exploit spatial and temporal heterogeneity in the availability of moist conditions--has allowed this invasive anuran to successfully colonize arid habitats in Australia. This finding illustrates that risk assessment protocols need to recognise that under some circumstances an introduced species may be able to thrive in conditions far removed from any that it experiences in its native range.

  13. Bioaccumulation of arsenic from water and sediment by a deposit-feeding polychaete (Arenicola marina): A biodynamic modelling approach

    Energy Technology Data Exchange (ETDEWEB)

    Casado-Martinez, M.C., E-mail: c.casado-martinez@nhm.ac.uk [Department of Zoology, Natural History Museum, Cromwell Rd, London SW7 5BD (United Kingdom); Smith, B.D. [Department of Zoology, Natural History Museum, Cromwell Rd, London SW7 5BD (United Kingdom); Luoma, S.N. [John Muir Institute of the Environment, University of California at Davis, Davis, CA 95616 (United States); Rainbow, P.S. [Department of Zoology, Natural History Museum, Cromwell Rd, London SW7 5BD (United Kingdom)

    2010-06-01

    Arsenic bioaccumulation in the deposit-feeding polychaete Arenicola marina has been investigated using biodynamic modelling. Radiotracer techniques were used to determine the rates of uptake of As as arsenate from water and sediment and its subsequent efflux in the laboratory. Lugworms accumulated As from solution linearly at concentrations of 2-20 {mu}g l{sup -1}, with a corresponding uptake rate constant of 0.1648 {+-} 0.0135 l g{sup -1} d{sup -1}. 7.8 {+-} 0.8% (assimilation efficiency) of the As ingested bound to sediments was retained after egestion of unassimilated metal. Elimination of As followed a two-compartment model, with mean efflux rate constants (from the slow pool) very similar for As accumulated from solution and ingested sediments (0.0449 {+-} 0.0034 and 0.0478 {+-} 0.0225 d{sup -1}, respectively) and a corresponding biological half-time of roughly 15 d. A biodynamic model was constructed and validated through the comparison of biodynamic model predictions against measured bioaccumulated concentrations in lugworms from five UK estuaries. The model accurately predicted bioaccumulated As concentrations in lugworms using mean values of relevant physiological parameters (uptake rate, efflux rate and growth rate constants), a site-specific ingestion rate (calculated according to mean worm size and sediment organic matter content and expressed as the rate of ingestion of the mass of fine sediment), a site-specific sediment concentration measured after HCl extraction, and a standard dissolved As concentration. This combination of parameters showed that sediment ingestion contributed 30-60% of the total As accumulated by lugworms at the studied sites, depending on the different geochemistry at each site. This study showed that it is difficult to predict accurately As bioaccumulation at sites with different chemistries, unless that chemistry is taken into account.

  14. Acute thermal stressor increases glucocorticoid response but minimizes testosterone and locomotor performance in the cane toad (Rhinella marina.

    Directory of Open Access Journals (Sweden)

    Edward J Narayan

    Full Text Available Climatic warming is a global problem and acute thermal stressor in particular could be considered as a major stressor for wildlife. Cane toads (Rhinella marina have expanded their range into warmer regions of Australia and they provide a suitable model species to study the sub-lethal impacts of thermal stressor on the endocrine physiology of amphibians. Presently, there is no information to show that exposure to an acute thermal stressor could initiate a physiological stress (glucocorticoid response and secondly, the possible effects on reproductive hormones and performance. Answering these questions is important for understanding the impacts of extreme temperature on amphibians. In this study, we experimented on cane toads from Queensland, Australia by acclimating them to mildly warm temperature (25°C and then exposing to acute temperature treatments of 30°, 35° or 40°C (hypothetical acute thermal stressors. We measured acute changes in the stress hormone corticosterone and the reproductive hormone testosterone using standard capture and handling protocol and quantified the metabolites of both hormones non-invasively using urinary enzyme-immunoassays. Furthermore, we measured performance trait (i.e. righting response score in the control acclimated and the three treatment groups. Corticosterone stress responses increased in all toads during exposure to an acute thermal stressor. Furthermore, exposure to a thermal stressor also decreased testosterone levels in all toads. The duration of the righting response (seconds was longer for toads that were exposed to 40°C than to 30°, 35° or 25°C. The increased corticosterone stress response with increased intensity of the acute thermal stressor suggests that the toads perceived this treatment as a stressor. Furthermore, the results also highlight a potential trade-off with performance and reproductive hormones. Ultimately, exposure acute thermal stressors due to climatic variability could impact

  15. Desiccation risk drives the spatial ecology of an invasive anuran (Rhinella marina in the Australian semi-desert.

    Directory of Open Access Journals (Sweden)

    Reid Tingley

    Full Text Available Some invasive species flourish in places that impose challenges very different from those faced in their native geographic ranges. Cane toads (Rhinella marina are native to tropical and subtropical habitats of South and Central America, but have colonised extremely arid regions over the course of their Australian invasion. We radio-tracked 44 adult cane toads at a semi-arid invasion front to investigate how this invasive anuran has managed to expand its geographic range into arid areas that lie outside of its native climatic niche. As predicted from their low physiological control over rates of evaporative water loss, toads selected diurnal shelter sites that were consistently cooler and damper (and thus, conferred lower water loss rates than nearby random sites. Desiccation risk also had a profound influence on rates of daily movement. Under wet conditions, toads that were far from water moved further between shelter sites than did conspecifics that remained close to water, presumably in an attempt to reach permanent water sources. However, this relationship was reversed under dry conditions, such that only toads that were close to permanent water bodies made substantial daily movements. Toads that were far from water bodies also travelled along straighter paths than did conspecifics that generally remained close to water. Thus, behavioural flexibility--in particular, an ability to exploit spatial and temporal heterogeneity in the availability of moist conditions--has allowed this invasive anuran to successfully colonize arid habitats in Australia. This finding illustrates that risk assessment protocols need to recognise that under some circumstances an introduced species may be able to thrive in conditions far removed from any that it experiences in its native range.

  16. High mortality of Zostera marina under high temperature regimes but minor effects of the invasive macroalgae Gracilaria vermiculophylla

    Science.gov (United States)

    Höffle, H.; Thomsen, M. S.; Holmer, M.

    2011-03-01

    The present study tested for density-dependent effects of the invasive drift macroalgae Gracilaria vermiculophylla (Ohmi) Papenfuss on growth and survival of the native eelgrass, Zostera marina L., under different temperature levels. Three weeks laboratory experiments were conducted in Odense, Denmark, combining three algae densities (control, low 1.9 kg WW m -2, high 4.5 kg WW m -2) with typical Danish summer temperatures (18 °C) and elevated temperatures (21 °C and 27 °C). There was a significant effect of temperature on shoot survival with on average 68% mortality in the high temperature treatment but almost no mortality at the two lower temperatures. The higher mortality was probably caused by high sulphide levels in the sediment pore water (0.6 mmol l -1 at 18 °C compared to 3.7 mmol l -1 at 27 °C). Above-ground growth of the surviving shoots was also significantly affected by temperature, with leaf elongation rates being negatively affected, while the leaf plastochrone interval increased. Relative growth rate was significantly higher at 21 °C than at 18 °C or 27 °C, whereas rhizome elongation was significantly lowest at 27 °C. Elemental sulphur content in the plant tissues increased significantly with temperature and was up to 34 times higher (S 0 in rhizomes) at 27 °C compared to the lower temperatures. In contrast to the temperature effects, cover by G. vermiculophylla did not cause significant effects on any seagrass responses. However, there was a (non-significant) negative effect of algal cover at the highest temperature, where the seagrass is already stressed. The latter results suggest that more studies should test for interaction effects between temperature and other anthropogenic stressors given that temperature is predicted to increase in the near future.

  17. Sexual selection in cane toads Rhinella marina: A male’s body size affects his success and his tactics

    Directory of Open Access Journals (Sweden)

    Haley BOWCOCK, Gregory P. BROWN, Richard SHINE

    2013-12-01

    Full Text Available Male body size can play an important role in the mating systems of anuran amphibians. We conducted laboratory-based trials with cane toads Rhinella (Bufo marina from an invasive population in the wet-dry tropics of northern Australia, to clarify the effects of a male's body size on his reproductive success and behavior (mate choice. Males were stimulated with a synthetic hormone to induce reproductive readiness. Larger body size enhanced a male toad's ability to displace a smaller rival from amplexus, apparently because of physical strength: more force was required to dislodge a larger than a smaller amplectant male. A male’s body size also affected his mate-choice criteria. Males of all body sizes were as likely to attempt amplexus with another male as with a female of the same size, and preferred larger rather than smaller sexual targets. However, this size preference was stronger in larger males and hence, amplexus was size-assortative. This pattern broke down when males were given access to already-amplectant male-female pairs: males of all body sizes readily attempted amplexus with the pair, with no size discrimination. An amplectant pair provides a larger visual stimulus, and prolonged amplexus provides a strong cue for sex identification (one of the individuals involved is almost certainly a female. Thus, a male cane toad’s body size affects both his ability to defeat rivals in physical struggles over females, and the criteria he uses when selecting potential mates, but the impacts of that selectivity depend upon the context in which mating occurs [Current Zoology 59 (6: 747–753, 2013].

  18. Hydrochemical features of groundwater in a coastal gypsum karst (Marina di Lesina, Gargano, Southern Italy) in relation to tides

    Science.gov (United States)

    Fidelibus, Maria Dolores; Campana, Claudia

    2014-05-01

    As part of a complex monitoring aimed at collecting suitable data for conceptual and numerical modelling of the coastal gypsum karst of Marina di Lesina (Gargano, Southern Italy), 64 groundwater samples were collected during three surveys at different depths from 9 monitoring wells aligned along two transects. The transects, perpendicular to the Acquarotta canal, are less of 1 Km long. The canal is directly connected to the sea and to the Lesina Lagoon and behaves as an oscillating border following sea tides. The sampling campaigns were carried out concurrently to phases of increasing, decreasing, and low tide and provide different frameworks of the chemical composition of ground waters. TDS (Total Dissolved Solids) of ground water samples ranges from 0.2 g/l to 35 g/l and increases generally along the flow lines towards the canal, and downward. The concentrations of the major ions deviate from theoretical ones defined by non-reactive mixing lines, which have as saline end-member either standard seawater or local seawater sampled offshore.Owing to the multi-component character of the hydrochemical system, the cation concentrations are controlled by competition among concurrent water-rock interaction processes that overlap to non-reactive freshwater-saltwater (FW-SW) mixing, being even triggered and/or enhanced by the same mixing, with feedback loops: gypsum and calcite dissolution, ion exchange (with direction depending on hydrodynamic conditions mainly driven by tides, and justified due to the presence of clay in the gypsum bedrock), and dedolomitization. The geochemical study also highlights the involvement of saline ground waters belonging to regional circuits that develop in the huge Mesozoic carbonate basement. The study highlights that in the saturated thickness of the gypsum coastal aquifer closer to the coast, the hydrochemical system is extremely reactive and strongly influenced by the different groundwater hydraulic conditions induced by the tide phases.

  19. Soil and Rhizosphere Associated Fungi in Gray Mangroves (Avicennia marina) from the Red Sea — A Metagenomic Approach

    Science.gov (United States)

    Simões, Marta Filipa; Antunes, André; Ottoni, Cristiane A.; Amini, Mohammad Shoaib; Alam, Intikhab; Alzubaidy, Hanin; Mokhtar, Noor-Azlin; Archer, John A.C.; Bajic, Vladimir B.

    2015-01-01

    Covering a quarter of the world’s tropical coastlines and being one of the most threatened ecosystems, mangroves are among the major sources of terrestrial organic matter to oceans and harbor a wide microbial diversity. In order to protect, restore, and better understand these ecosystems, researchers have extensively studied their microbiology, yet few surveys have focused on their fungal communities. Our lack of knowledge is even more pronounced for specific fungal populations, such as the ones associated with the rhizosphere. Likewise, the Red Sea gray mangroves (Avicennia marina) remain poorly characterized, and understanding of their fungal communities still relies on cultivation-dependent methods. In this study, we analyzed metagenomic datasets from gray mangrove rhizosphere and bulk soil samples collected in the Red Sea coast, to obtain a snapshot of their fungal communities. Our data indicated that Ascomycota was the dominant phylum (76%–85%), while Basidiomycota was less abundant (14%–24%), yet present in higher numbers than usually reported for such environments. Fungal communities were more stable within the rhizosphere than within the bulk soil, both at class and genus level. This finding is consistent with the intrinsic patchiness in soil sediments and with the selection of specific microbial communities by plant roots. Our study indicates the presence of several species on this mycobiome that were not previously reported as mangrove-associated. In particular, we detected representatives of several commercially-used fungi, e.g., producers of secreted cellulases and anaerobic producers of cellulosomes. These results represent additional insights into the fungal community of the gray mangroves of the Red Sea, and show that they are significantly richer than previously reported. PMID:26549842

  20. Soil and Rhizosphere Associated Fungi in Gray Mangroves (Avicennia marina) from the Red Sea--A Metagenomic Approach.

    Science.gov (United States)

    Simões, Marta Filipa; Antunes, André; Ottoni, Cristiane A; Amini, Mohammad Shoaib; Alam, Intikhab; Alzubaidy, Hanin; Mokhtar, Noor-Azlin; Archer, John A C; Bajic, Vladimir B

    2015-10-01

    Covering a quarter of the world's tropical coastlines and being one of the most threatened ecosystems, mangroves are among the major sources of terrestrial organic matter to oceans and harbor a wide microbial diversity. In order to protect, restore, and better understand these ecosystems, researchers have extensively studied their microbiology, yet few surveys have focused on their fungal communities. Our lack of knowledge is even more pronounced for specific fungal populations, such as the ones associated with the rhizosphere. Likewise, the Red Sea gray mangroves (Avicennia marina) remain poorly characterized, and understanding of their fungal communities still relies on cultivation-dependent methods. In this study, we analyzed metagenomic datasets from gray mangrove rhizosphere and bulk soil samples collected in the Red Sea coast, to obtain a snapshot of their fungal communities. Our data indicated that Ascomycota was the dominant phylum (76%-85%), while Basidiomycota was less abundant (14%-24%), yet present in higher numbers than usually reported for such environments. Fungal communities were more stable within the rhizosphere than within the bulk soil, both at class and genus level. This finding is consistent with the intrinsic patchiness in soil sediments and with the selection of specific microbial communities by plant roots. Our study indicates the presence of several species on this mycobiome that were not previously reported as mangrove-associated. In particular, we detected representatives of several commercially-used fungi, e.g., producers of secreted cellulases and anaerobic producers of cellulosomes. These results represent additional insights into the fungal community of the gray mangroves of the Red Sea, and show that they are significantly richer than previously reported. Copyright © 2015 The Authors. Production and hosting by Elsevier Ltd.. All rights reserved.

  1. Poliquetos (Annelida: Polychaeta como indicadores biológicos de contaminación marina: casos en Colombia

    Directory of Open Access Journals (Sweden)

    Vanessa Fernández Rodríguez

    2015-06-01

    Full Text Available El concepto de Indicador Biológico ha sido usado sin mayor precaución al momento de emplear ciertos organismos en programas de monitoreo ambiental, lo cual genera confusión. Uno de los objetivos de este trabajo fue resaltar la importancia en la consolidación de una definición precisa de este concepto a partir de información disponible, proponiendo una definición para el mismo. Las características ecológicas de los poliquetos permiten que, al estar en contacto permanente con diferentes tipos de contaminantes, respondan bioacumulando, disminuyendo o aumentando su abundancia, según sea la especie, hecho que posiciona este tipo de organismos como potenciales indicadores de contaminación marina. En este artículo se presenta de manera concreta un análisis de la literatura disponible para poliquetos en el campo de los indicadores biológicos, resaltando cómo éstos han sido usados en diferentes metodologías, con ejemplos a internacionales, así como una selección especial para Colombia. De los resultados más sobresalientes se encontró que Capitella capitata es la especie más estudiada al estar asociada con ambientes contaminados a causa del incremento de materia orgánica y es la única especie reportada en el país como indicador biológico usando las técnicas clásicas de bioindicación. Finalmente, se reitera la importancia de iniciar investigaciones sobre los aspectos ecológicos, ecotoxicológicos y bioensayos de laboratorio con otras especies de poliquetos para validar cuáles especies y por qué pueden ser consideradas como indicadores biológicos para el país.

  2. Arquitectura y poder local: los orígenes de la comandancia de marina de Las Palmas

    Directory of Open Access Journals (Sweden)

    Javier Márquez Quevedo

    2013-10-01

    Full Text Available La construcción de edificaciones representativas del poder ha sido siempre una constante. En el caso de Canarias, una región pobre y alejada del centro político de Estado, esta arquitectura tomó una gran relevancia debido a su escasez. En una etapa de creciente rivalidad entre la capital provincial, Santa Cruz de Tenerife, y Las Palmas, ciudad que despuntaba con el auge del Puerto de La Luz, el deseo de contar cuanto antes con un vistoso edificio para la Comandancia de Marina tuvo un evidente objetivo político. Este artículo es un breve repaso a los orígenes de esta construcción y sus dificultades para realizarla.The representative authority building has ever been manifest. In Canary Islands, needy and distant region from metropolis, this architecture obtains large importance owing to her shortness. In the times of increasing competition between the chief town, Santa Cruz de Tenerife, and Las Palmas, one place that expanding through Puerto de La Luz’s summit, to have a desire to count upon a showy commandership building rapidly it was an obvious political objective. In this essay, we will analyse the origins of this building and the objections in order to achieve it.

  3. COMPORTAMIENTO ESTACIONAL DE LA CAPA DE MEZCLA MARINA EN LA REGIÓN NORTE DEL GOLFO DE CALIFORNIA

    Directory of Open Access Journals (Sweden)

    Emilio Palacios Hernández

    2016-01-01

    Full Text Available Se realiza l a descripción de la profundidad de la capa de mezcla marina superficial (PCM en la Región Norte del Golfo de California (RNGC, utilizando datos hidrográficos obtenidos en 17 cruceros oceanográficos desde 1971 hasta 1996. La profundidad de la capa se estima directament e de los perfiles de densidad . El promedio de distribución espacial muestra un máximo bien definido en la salida del canal de Ballenas y un mínimo en las costas de Sonora y de Baja California donde la capa de mezcla no puede ser más profunda ya que son lugares demasiado someros y la mez cla cubre toda la profundidad del lugar. La PCM en la RNGC tiene una señal estacional con una transición en abril y otra en octubre. En verano (ju l io a septiembre la profundidad de la capa de mezcla se caracteriza por ser demasiado so mera mostrando un máx imo de 48 m y un mínimo de 10 m, mientras que en invierno (enero a marz o se prof undiza a un máximo de hasta 100 m . Existe una anomalía en el evento de ma rzo de 1973 la cual muestra estima ciones de PCM mayores que las esperadas, estas anomalías al parecer son causadas por el evento interanual El Niño (EN 1972 - 1973, ya que no se pudo encontrar evidencia similar del fenómeno en otros marzos.

  4. Evaluation of anti-HIV-1 activity of a new iridoid glycoside isolated from Avicenna marina, in vitro.

    Science.gov (United States)

    Behbahani, Mandana

    2014-11-01

    This study was carried out to check the efficacy of methanol seed extract of Avicenna marina and its column chromatographic fractions on Peripheral Blood Mono nuclear Cells (PBMCs) toxicity and HIV-1 replication. The anti-HIV-1 activities of crude methanol extract and its fractions were performed by use of real-time polymerase chain reaction (PCR) assay and HIV-1 p24 antigen kit. A time of drug addiction approach was also done to identify target of anti-HIV compound. The activity of the extracts on CD4, CD3, CD19 and CD45 expression in lymphocytes population was performed by use of flow cytometry. The most active anti-HIV agent was detected by spectroscopic analysis as 2'-O-(4-methoxycinnamoyl) mussaenosidic acid. The apparent effective concentrations for 50% virus replication (EC50) of methanol extract and iridoid glycoside were 45 and 0.1 μg/ml respectively. The iridoid glycoside also did not have any observable effect on the proportion of CD4, CD3, CD19 and CD45 cells or on the intensity of their expressions on PBMCs. In addition, the expression level of C-C chemokine receptor type 5 (CCR5) and chemokine receptor type 4 (CXCR4) on CD4(+) T cells were decreased in cells treated with this iridoid glycoside. The reduction of these two HIV coreceptors and the result of time of addition study demonstrated that this iridoid glycoside restricts HIV-1 replication on the early stage of HIV infection. Copyright © 2014 Elsevier B.V. All rights reserved.

  5. Differences in benthic fauna and sediment among mangrove ( Avicennia marina var. australasica) stands of different ages in New Zealand

    Science.gov (United States)

    Morrisey, D. J.; Skilleter, G. A.; Ellis, J. I.; Burns, B. R.; Kemp, C. E.; Burt, K.

    2003-03-01

    Management of coastal environments requires understanding of ecological relationships among different habitats and their biotas. Changes in abundance and distribution of mangroves, like those of other coastal habitats, have generally been interpreted in terms of changes in biodiversity or fisheries resources within individual stands. In several parts of their range, anthropogenically increased inputs of sediment to estuaries have led to the spread of mangroves. There is, however, little information on the relative ecological properties, or conservational values, of stands of different ages. The faunal, floral and sedimentological properties of mangrove ( Avicennia marina var. australasica) stands of two different ages in New Zealand has been compared. Older (>60 years) and younger (3-12 years) stands showed clear separation on the basis of environmental characteristics and benthic macrofauna. Numbers of faunal taxa were generally larger at younger sites, and numbers of individuals of several taxa were also larger at these sites. The total number of individuals was not different between the two age-classes, largely due to the presence of large numbers of the surface-living gastropod Potamopyrgus antipodarum at the older sites. It is hypothesized that as mangrove stands mature, the focus of faunal diversity may shift from the benthos to animals living on the mangrove plants themselves, such as insects and spiders, though these were not included in the present study. Differences in the faunas were coincident with differences in the nature of the sediment. Sediments in older stands were more compacted and contained more organic matter and leaf litter. Measurement of leaf chemistry suggested that mangrove plants in the younger stands were able to take up more N and P than those in the older stands.

  6. Soil and Rhizosphere Associated Fungi in Gray Mangroves (Avicennia marina from the Red Sea — A Metagenomic Approach

    Directory of Open Access Journals (Sweden)

    Marta Filipa Simões

    2015-10-01

    Full Text Available Covering a quarter of the world’s tropical coastlines and being one of the most threatened ecosystems, mangroves are among the major sources of terrestrial organic matter to oceans and harbor a wide microbial diversity. In order to protect, restore, and better understand these ecosystems, researchers have extensively studied their microbiology, yet few surveys have focused on their fungal communities. Our lack of knowledge is even more pronounced for specific fungal populations, such as the ones associated with the rhizosphere. Likewise, the Red Sea gray mangroves (Avicennia marina remain poorly characterized, and understanding of their fungal communities still relies on cultivation-dependent methods. In this study, we analyzed metagenomic datasets from gray mangrove rhizosphere and bulk soil samples collected in the Red Sea coast, to obtain a snapshot of their fungal communities. Our data indicated that Ascomycota was the dominant phylum (76%–85%, while Basidiomycota was less abundant (14%–24%, yet present in higher numbers than usually reported for such environments. Fungal communities were more stable within the rhizosphere than within the bulk soil, both at class and genus level. This finding is consistent with the intrinsic patchiness in soil sediments and with the selection of specific microbial communities by plant roots. Our study indicates the presence of several species on this mycobiome that were not previously reported as mangrove-associated. In particular, we detected representatives of several commercially-used fungi, e.g., producers of secreted cellulases and anaerobic producers of cellulosomes. These results represent additional insights into the fungal community of the gray mangroves of the Red Sea, and show that they are significantly richer than previously reported.

  7. Empleo de polímeros conductores para mejorar la protección contra la corrosión en pinturas marinas

    OpenAIRE

    Souto Blázquez, Marina

    2007-01-01

    El objetivo de este proyecto es el de analizar el comportamiento ante la corrosión marina en pinturas comerciales tras la adición de distintos polímeros conductores. En primer lugar se realizará la caracterización fisicoquímica de las pinturas creadas mediante termogravimetría (TGA), espectroscopia infrarroja (FTIR) y microscopía óptica (MO), así como la determinación de las propiedades mecánicas de las películas mediante ensayos tracción-deformación. Posteriormente, se apli...

  8. Uptake of curium (/sup 244/Cm) by five benthic marine species (Arenicola marina, Cerastoderma edule, Corophium volutator, Nereis diversicolor and Scrobicularia plana): comparison with americium and plutonium

    Energy Technology Data Exchange (ETDEWEB)

    Miramand, P.; Germain, P.; Arzur, J.C.

    1987-01-01

    Curium (/sup 244/Cm) uptake from contaminated sea water was studied in five benthic marine species: two bivalve molluscs (Scrobicularia plana and Cerastoderma edule), two polychaete annelids (Arenicola marina and Nereis diversicolor) and one amphidpod crustacean (Corophium volutator). The concentrations in the whole organisms relative to the concentration in the sea water (concentration factors) were: 700 for the amphipods (after 11 d of accumulation), 140 for the cockles (after 28 d), 80 for the scrobicularia (after 23d) and approx. 30 for the two annelids (after > 20 d). All species except S. plana accumulated americium and curium similarly; S. plana accumulated similar amounts of curium and plutonium.

  9. El valor económico del Blue Carbon en Colombia: Beneficios de la captura y almacenamiento de carbono provistos por las Áreas Marinas Protegidas

    OpenAIRE

    Zárate, Tatiana; Maldona, Jorge Higinio

    2014-01-01

    El Subsistema de Áreas Marinas Protegidas que se está implementando en Colombia apunta a la conservación de ecosistemas marinos y costeros claves para la provisión de diversos servicios ambientales, que son la base del desarrollo de distintas actividades económicas en el país. El objetivo de este documento es valorar los servicios asociados a captura y almacenamiento de carbono oceánico –Blue Carbon- provistos por el Subsistema. A través de la construcción de una función de beneficios y la es...

  10. Wild Cane Toads (Rhinella marina) Expel Foreign Matter from the Coelom via the Urinary Bladder in Response to Internal Injury, Endoparasites and Disease

    OpenAIRE

    Crystal Kelehear; Hugh I. Jones; Wood, Benjamin A.; Richard Shine

    2015-01-01

    Dissections of >1,200 wild-caught cane toads (Rhinella marina) in tropical Australia confirm a laboratory report that anurans can expel foreign objects from the coelom by incorporating them into the urinary bladder. The foreign objects that we found inside bladders included a diverse array of items (e.g., grass seeds, twigs, insect prey, parasites), many of which may have entered the coelom via rupture of the gut wall. In some cases, the urinary bladder was fused to other organs including liv...

  11. Estudio de las diferentes alternatives en materia de recuperación de energia para una Estación Desaladora de Agua Marina

    OpenAIRE

    Bonet de Tena, Albert

    2011-01-01

    La desalación de agua de mar tiene por objeto producir agua apta para cualquier uso a partir de agua marina. El agua de mar se caracteriza por su elevado contenido de sales en disolución, las cuales deben ser eliminadas en su práctica totalidad. Según las leyes físicas que rigen el comportamiento de las disoluciones, esta separación requiere una considerable aplicación de energía. El presente proyecto pretende sintetizar las bases físicas del consumo energético en la desalac...

  12. ENRIQUECIMIENTO AMBIENTAL DE NUTRIA MARINA Lontra felina (MOLINA 1782) EN EL PARQUE ZOOLÓGICO HUACHIPA ENTRE FEBRERO Y MARZO DEL 2007

    OpenAIRE

    Ruiz Ramos, Elisa Diana; Parque Zoológico Huachipa (Perú).; Díaz Carrillo, Edward Iván; Parque Zoológico Huachipa (Perú).

    2008-01-01

    El enriquecimiento ambiental es una herramienta empleada para mejorar situaciones de aburrimiento, estrés y así evitar enfermedades causadas por la inactividad de la vida en cautiverio en la fauna silvestre. El presente trabajo se desarrolló con 2 individuos de nutria marina Lontra felina (Molina 1782) en cautiverio, durante cinco semanas: en la primera semana se realizó la determinación de los patrones de comportamiento mediante un muestreo Ad libitum, y durante las cuatro semanas restantes ...

  13. Submissão e subversão : a complexidade dos relacionamentos entre homens e mulheres em alguns contos de Marina Colasanti

    OpenAIRE

    Angela Simone Ronqui Oliva

    2016-01-01

    Esta tese tem o objetivo de verificar a temática da complexidade dos relacionamentos entre homens e mulheres, presente em alguns contos de Marina Colasanti, escritora contemporânea da literatura brasileira. Parte-se da premissa que a autora, durante quarenta anos de produção literária (1975-2015) percorre, reiteradamente, esse tema em sua produção contística. Sendo assim, a fundamentação teórica da pesquisa foi baseada em estudos acerca da relação entre homens e mulheres, principalmente, na H...

  14. Visiones desde dentro. La insurrección cubana a través del Diario de la Marina y Bohemia (1956-1958

    Directory of Open Access Journals (Sweden)

    Patricia Calvo GONZÁLEZ

    2014-12-01

    Full Text Available El artículo reflexiona sobre el papel el papel desempeñado por la prensa cubana durante la insurrección de finales de los años cincuenta. Para ello nos hemos centrado en el análisis de dos publicaciones específicas: Diario de la Marina y Bohemia. Su diferente periodicidad y línea editorial permiten obtener una visión global del funcionamiento mediático de la isla durante el levantamiento que finalmente derrocaría a Batista y daría paso a la Revolución Cubana.

  15. Emotive language features of text as an indicator of the author's attitude in the poem “Homesickness” by Marina Tsvetaeva

    Directory of Open Access Journals (Sweden)

    Demchenko Yuriy Olegivich

    2017-10-01

    Full Text Available This article demonstrates how in Marina Tsvetaeva’s poem “Homesickness” the author’s attempt to present her mental state as an attitude of well-worn indifference to the positive and negative aspects of life transforms into an intense emotional reaction. It reveals the methods of representing a subject inside and outside of time and space. This article also discusses the forms of explication and implication of its passivity and isolation. Finally, it analyzes the components of the text which create and strengthen the all-encompassing negative assessment caused by the emotions experienced.

  16. Evaluación nutricional y propiedades biológicas de algas marinas comestibles. Estudios "in vitro" e "in vivo"

    OpenAIRE

    Gómez Ordóñez, Eva

    2012-01-01

    El objetivo principal de esta tesis doctoral es la caracterización y evaluación in vitro e in vivo de la calidad nutricional de algas marinas españolas como fuente de compuestos biológicamente activos, principalmente polisacáridos sulfatados. Se ha analizado la composición química de las algas pardas y rojas de partida y especialmente la fibra alimentaria. Además mediante una extracción química secuencial se han obtenido fracciones solubles de los principales polisacáridos de la pared celular...

  17. Lista de esponjas marinas asociadas al arrecife Tuxpan, Veracruz, México Checklist of marine sponges from Tuxpan Reef, Veracruz, Mexico

    OpenAIRE

    Carlos González-Gándara; Abelardo Patiño-García; Uribe Asís-Anastasio; Arturo Serrano; Patricia Gómez

    2009-01-01

    Se presenta la lista de esponjas marinas (Porifera: Demospongiae) del arrecife Tuxpan, Veracruz, México, colectadas en 2004, 2005 y 2006 mediante buceo libre y con equipo autónomo SCUBA. Los resultados muestran la presencia de 18 especies pertenecientes a 13 géneros y 13 familias, 17 de estas especies son nuevos registros para los arrecifes coralinos del norte de Veracruz y una (Aplysina cauliformis Carter, 1882) para el estado. La información puede auxiliar para definir las estrategias de ma...

  18. Estudio comparado de la alimentación entre algunos predadores de alto nivel trófico de la comunidad marina del norte y centro de Patagonia

    OpenAIRE

    Koen Alonso, Mariano

    1999-01-01

    El estudio comparado de la alimentación de predadores de alto nivel trófico permite evaluar la forma en que estas especies utilizan los recursos tróficos, posibilitando así el análisis de las potenciales relaciones de competencia y el papel que estos predadores pueden tener en la regulación y estructuración de la comunidad a la cuál pertenecen. La comunidad marina del norte y centro de Patagonia se estructura alrededor del sistema tritrófico conformado por la anchoita (Engraulis anchoíta), el...

  19. Turismo versus pesca artesanal. A propósito de La Reserva Marina de la Isla de La Graciosa y los Islotes del Norte de Lanzarote

    OpenAIRE

    Gloria Cabrera Socorro; Alfredo Cabrera Socorro

    2004-01-01

    La Reserva Marina de La Graciosa y los Islotes del Norte de Lanzarote (RMLGINL) se creó en 1995 y, desde entonces, las actividades pesqueras de la población local y su cultura del mar han ido decreciendo progresivamente mientras que las actividades turísticas siguen emergiendo como la panacea en una pequeña isla, como La Graciosa, con no más de 600 habitantes. En este artículo tratamos de analizar cómo estos dos procesos están relacionados y qué papel está jugando la reserva marin...

  20. La acuariofilia de especies ornamentales marinas: un mercado de retos y oportunidades Ornamental marine fishkeeping: a trade of challenges and opportunities

    Directory of Open Access Journals (Sweden)

    Fabiola Lango Reynoso

    2012-03-01

    Full Text Available La industria de la acuariofilia es un mercado que se encuentra en expansión. Aun cuando no existe información exacta respecto a cifras y valores del comercio internacional, se calcula que esta industria genera ganancias sobre US$ 300 millones con una tasa de crecimiento anual del 14%. De esta cifra, aproximadamente US$ 28 a 44 millones corresponden a peces ornamentales de origen marino. No obstante, una de las controversias que ha generado el comercio de especies marinas es que casi la totalidad de los organismos son capturados del medio natural, y en muchos casos de manera ilegal. En este trabajo se realiza una revisión que incluye: historia de la acuariofilia, principales especies ornamentales de importancia comercial, comercio internacional, riesgos del comercio de especies marinas, estrategias de conservación y situación actual en México. En este último tema, se abordan aspectos que describen el desarrollo y perspectivas del mercado de la acuariofilia marina y algunas iniciativas de instituciones académicas y privadas dirigidas al desarrollo de la acuicultura sostenible de especies marinas de ornato de importancia comercial en el país.The aquariums industry is a trade that is expanding. Although there is no accurate information regarding the figures of the aquarium industry in the international scenario, it is estimated to generate revenues over US$ 300 million with an annual growth rate of 14%. Approximately US$ 28 to 44 million of this amount is generated by the ornamental marine fish trade. However, the trade in marine species has aroused controversy regarding the extraction of almost all traded marine organisms from wild populations and, in many cases, illegally. This paper presents a review that includes: the ornamental marine fishkeeping history, the species of commercial importance, the international trade scenario, risks of the aquarium industry, conservation strategies, and the current situation of this trade in Mexico. The

  1. Proyecto constructivo de una rotonda en el cruce entre las calles Cobalto, Femades y Marina entre Hospitalet de Llobregat y Cornellà de Llobregat

    OpenAIRE

    Muñoz González, Oliver

    2012-01-01

    El objeto del proyecto es la construcción de una rotonda en el cruce entre las calles de la Marina, Cobalto y Femades ubicadas entre los municipios de Cornellà de Llobregat y Hospitalet de Llobregat. Así mismo también se proyecta la implantación de un carril bici a lo largo de la calle Femades. Esto es así ya que el Plan director de la Bicicleta de Hospitalet prevé la construcción de éste a partir de 2012. La rotonda se espera que proporcione al cruce una mayor fluidez de circu...

  2. Desarrollo de un nuevo bioensayo para detección de toxinas marinas en moluscos bivalvos utilizando el modelo del pez cebra (Danio Rerio)

    OpenAIRE

    Fellenz, Nicolás Antonio; Piñuel, Lucrecia; Boeri, Patricia; Fernández, Carolina; Barrio, Natalia; Barrio, Daniel Alejandro

    2017-01-01

    La extracción, producción y procesamiento de moluscos bivalvos presenta un inconveniente para la calidad e inocuidad agroalimentaria, dado que pueden estar contaminados con toxinas marinas. El consumo de mariscos contaminados con dichas sustancias provoca intoxicaciones graves y constituye un problema para la salud de la población. Por eso, es necesario contar con un método rápido, sensible y específico para determinar su presencia. El bioensayo en ratón, establecido como método oficial por l...

  3. Evaluacion base para una eventual Área Marina Protegida (AMP) en el norte de Chile (III Región).

    OpenAIRE

    Vásquez, Julio

    2002-01-01

    En Chile, las áreas marinas protegidas (AMP) constituyen un herramienta de conservación creada recientemente a través de la Ley General de Pesca y Acuicultura. A lo largo de más de 4000 km de costa, sólo existe una AMP en el litoral de Chile continental, localizada en la costa de la II Región-Antofagasta. Esta AMP está orientada a la conservación y preservación de Argopecten purpuratus "Ostión del Norte".

  4. El efecto de la protección en las poblaciones de peces de la reserva marina de Ses Negres (Mediterráneo noroccidental, España)

    OpenAIRE

    Marc Rius

    2007-01-01

    Este trabajo es el resultado de un programa de seguimiento de 3 años estudiando el efecto que la reserva marina de Ses Negres ha tenido sobre las poblaciones de peces. La riqueza específica (basada en especies de peces consideradas indicadoras del efecto reserva), y la estructura de tallas de 4 especies vulnerables a la pesca con caña y arpón fueron estudiadas utilizando censos visuales llevados a cabo dentro y fuera de la reserva marina. El análisis de las poblaciones de peces dentro d...

  5. Caracterización toxicológica de las macroalgas marinas Hypnea spp y Sargasun spp para la futura utilización en la alimentación y la salud animal como humana

    OpenAIRE

    Vladimir Barrios González; Roberto Castillo; Ivanhoe González Sánchez; Lourdes Savón Valdés :

    2007-01-01

    El estudio y la investigación creciente en la industria alimenticia, farmacológica así como en la cosmeticología ha provocado que se analicen variantes novedosas como las macroalgas marinas. La moderna agricultura como ciencia, además del cultivo y explotación racional de los peces, crustáceos y moluscos, contempla una importante área de investigación y desarrollo como lo son las macroalgas marinas. Las algas constituyen el primer eslabón de la cadena trófica que más eficientemente aprovec...

  6. Nuevas citas y aportaciones corológicas para la flora bentónica marina del Atlántico de la Península Ibérica

    OpenAIRE

    Bárbara, Ignacio; Díaz Tapia, Pilar; Peteiro, César; Berecibar, Estibaliz; Peña, Viviana; Sánchez, Noemí; Tavares, Ana Mafalda; Santos, Rui; Secilla, Antonio; Riera Fernández, Pablo; Bermejo, Ricardo; García, Verónica

    2012-01-01

    Se dan a conocer nuevas localizaciones y datos corológicos para 98 especies (61 Rhodophyta, 22 Ochrophyta, 15 Chlorophyta) de algas bentónicas marinas recolectadas en el intermareal y submareal de más de 80 localidades de las costas atlánticas y cantábricas de la Península Ibérica. Polysiphonia devoniensis, P. fibrata y Zonaria tournefortii son novedad para Portugal y 5 especies (Bonnemaisonia hamifera, Calosiphonia vermicularis, P. devoniensis, Hincksia intermedia y Derbesia marina stadium H...

  7. Survey of helminths, ectoparasites, and chytrid fungus of an introduced population of cane toads, Rhinella marina (Anura: Bufonidae), from Grenada, West Indies.

    Science.gov (United States)

    Drake, Michael C; Zieger, Ulrike; Groszkowski, Andrew; Gallardo, Bruce; Sages, Patti; Reavis, Roslyn; Faircloth, Leslie; Jacobson, Krystin; Lonce, Nicholas; Pinckney, Rhonda; Cole, Rebecca A

    2014-10-01

    One hundred specimens of Rhinella marina , (Anura: Bufonidae) collected in St. George's parish, Grenada, from September 2010 to August 2011, were examined for the presence of ectoparasites and helminths. Ninety-five (95%) were parasitized by 1 or more parasite species. Nine species of parasites were found: 1 digenean, 2 acanthocephalans, 4 nematodes, 1 arthropod and 1 pentastome. The endoparasites represented 98.9% of the total number of parasite specimens collected. Grenada represents a new locality record for Mesocoelium monas, Raillietiella frenatus, Pseudoacanthacephalus sp., Aplectana sp., Physocephalus sp., Acanthacephala cystacanth, and Physalopteridae larvae. The digenean M. monas occurred with the highest prevalence of 82%, contrasting many studies of R. marina where nematodes dominate the parasite infracommunity. Female toads were found to have a significantly higher prevalence of Amblyomma dissimile than male toads. Only 2 parasites exhibited a significant difference between wet and dry season with Parapharyngodon grenadensis prevalence highest in the wet season and A. dissimile prevalence highest during the dry season. Additionally, A. dissimile was significantly more abundant during the dry season.

  8. Sequence analysis and structure prediction of enoyl-CoA hydratase from Avicennia marina: implication of various amino acid residues on substrate-enzyme interactions.

    Science.gov (United States)

    Jabeen, Uzma; Salim, Asmat

    2013-10-01

    Enoyl-CoA hydratase catalyzes the hydration of 2-trans-enoyl-CoA into 3-hydroxyacyl-CoA. The present study focuses on the correlation between the functional and structural aspects of enoyl-CoA hydratase from Avicennia marina. We have used bioinformatics tools to construct and analyze 3D homology models of A. marina enoyl-CoA hydratase (AMECH) bound to different substrates and inhibitors and studied the residues involved in the ligand-enzyme interaction. Structural information obtained from the models was compared with those of the reported crystal structures. We observed that the overall folds were similar; however, AMECH showed few distinct structural changes which include structural variation in the mobile loop, formation and loss of certain interactions between the active site residues and substrates. Some changes were also observed within specific regions of the enzyme. Glu106 is almost completely conserved in sequences of the isomerases/hydratases including AMECH while Glu86 which is the other catalytic residue in most of the isomerases/hydratases is replaced by Gly and shows no interaction with the substrate. Asp114 is located within 4Å distance of the catalytic water which makes it a probable candidate for the second catalytic residue in AMECH. Another prominent feature of AMECH is the presence of structurally distinct mobile loop having a completely different coordination with the hydrophobic binding pocket of acyl portion of the substrate. Copyright © 2013 Elsevier Ltd. All rights reserved.

  9. Identificación de las prioridades de conservación de la biodiversidad marina y costera en Costa Rica

    Directory of Open Access Journals (Sweden)

    Juan José Alvarado

    2011-06-01

    Full Text Available Costa Rica es un país reconocido por su alta diversidad de especies y ecosistemas, tanto en sus ambientes terrestres como marinos. A pesar de esta importancia, presenta un rezago en la conservación y manejo de la biodiversidad marina y costera, con respecto a la terrestre. Para el año 2006, la superficie marina protegida era de 5 208.8km² y 331.5km de línea costera, en 20 áreas silvestres protegidas. El país ha logrado importantes avances en la selección de sitios prioritarios para la conservación de la biodiversidad terrestre y de aguas continentales, aunque pocos esfuerzos se han realizado hasta el momento en la planificación marina. En este trabajo se muestra el análisis y resultados de un proceso de identificación de vacíos en la representatividad de la biodiversidad marina y costera en el sistema de áreas protegidas de Costa Rica. El estudio se construyó a partir de la información espacial disponible sobre la presencia y distribución de la biodiversidad marina y costera, el establecimiento de metas de conservación y el análisis de amenazas sobre la integridad ecológica de la biodiversidad. Posteriormente, se llevó a cabo la selección de sitios prioritarios mediante técnicas de optimización espacial, y la sobreposición con la actual capa de áreas marinas protegidas, para finalmente identificar los vacíos en la representatividad. En total, se identificaron 19 076km² de vacíos de conservación, 1 323km² en el Caribe y 17 753km² en el Pacífico. Se plantean recomendaciones dirigidas hacia la ampliación y fortalecimiento del sistema de áreas marinas protegidas del país, en el cual se usan los vacíos identificados como marco de referencia. Se espera que los resultados presentes sean parte de la base científica necesaria para la planificación de la conservación y uso sostenible de la biodiversidad marina en el país.Identification of Marine and Coastal biodiversity conservation priorities in Costa Rica. Costa

  10. A highly diverse molluscan assemblage associated with eelgrass beds (Zostera marina L. in the Alboran Sea: Micro-habitat preference, feeding guilds and biogeographical distribution

    Directory of Open Access Journals (Sweden)

    José L. Rueda

    2009-12-01

    Full Text Available The fauna of molluscs associated with deep subtidal Zostera marina beds (12-16 m in southern Spain (Alboran Sea has been characterised in terms of micro-habitat preference, feeding guilds and biogeographical affinity. The species list (162 taxa is based on sampling completed before the strong eelgrass decline experienced in 2005-2006, using different methods (small Agassiz trawl covering 222 m2 and quadrates covering 0.06 m2 and different temporal scales (months, day/night. Dominant epifaunal species are Jujubinus striatus, Rissoa spp. and Smaragdia viridis in the leaf stratum and Nassarius pygmaeus, Bittium reticulatum and Calliostoma planatum on the sediment. Nevertheless, the infauna dominated the epifauna in terms of number of individuals, including mainly bivalves (Tellina distorta, T. fabula, Dosinia lupinus. The epifauna of both the sediment and leaf strata included high numbers of species, probably due to the soft transition between vegetated and unvegetated areas. The dominant feeding guilds were deposit feeders, filter feeders and peryphiton grazers, but ectoparasites (eulimids, seagrass grazers (Smaragdia viridis and an egg feeder (Mitrella minor also occurred, unlike in other eelgrass beds of Europe. The molluscan fauna of these Z. marina beds is essentially derived from the local fauna, which includes many widely distributed species along European coasts, with a low representation of strictly Mediterranean or strictly Atlantic species. This fauna is richer than that found in other eelgrass beds of Europe, and deserves important attention for conservation.

  11. Combined impact of water column oxygen and temperature on internal oxygen status and growth of Zostera marina seedlings and adult shoots

    DEFF Research Database (Denmark)

    Raun, Ane-Marie Løvendahl; Borum, Jens

    2013-01-01

    Eelgrass (Zostera marina L.) occasionally experiences severe die-offs during warm summer periods with variable water column oxygen partial pressures (pO). Eelgrass is known to be very intolerant to tissue anoxia with reduced growth and increasing mortality after ≤12h anoxia in the dark at tempera......Eelgrass (Zostera marina L.) occasionally experiences severe die-offs during warm summer periods with variable water column oxygen partial pressures (pO). Eelgrass is known to be very intolerant to tissue anoxia with reduced growth and increasing mortality after ≤12h anoxia in the dark...... at temperatures of ≥25°C. In the present study we experimentally examine the impact of combined water column oxygen and temperature on oxygen dynamics in leaf meristems of seedlings and adult shoots to better understand how stressful environmental conditions affect eelgrass oxygen dynamics and subsequent growth...... and mortality. There was a strong interaction between water column oxygen and temperature on meristem pO implying that eelgrass is rather resistant to unfavorable oxygen conditions in winter but becomes increasingly vulnerable in summer, especially at high temperatures. At 25°C meristems became anoxic...

  12. Effects of A.marina-Derived Isoquercitrin on TNF-Related Apoptosis-Inducing Ligand Receptor (TRAIL-R) Expression and Apoptosis Induction in Cervical Cancer Cells.

    Science.gov (United States)

    Arumugam, Sathishkumar; Bandil, Kapil; Proksch, Peter; Murugiyan, Kalaiselvam; Bharadwaj, Mausumi

    2017-06-01

    TNF-related apoptosis-inducing ligand (TRAIL) is an anticancer agent, which has greater apoptosis inducing capacity, but most of the cancer cells become resistant to TRAIL-induced apoptosis. The combined treatment of TRAIL with natural products could restore the cancer cell sensitivity to recombinant human TRAIL (rhTRAIL) protein and might enhance the TNF-related apoptosis-inducing ligand receptor (TRAIL-R) expression. This investigation was aimed to isolate flavonoids from leaves of Avicennia marina and evaluate their potential for sensitization of rhTRAIL in human cervical cancer cells (SiHa). The methanolic extract of A.marina leaves were purified and structure was elucidated as isoquercitrin by NMR and LC-MS analysis. Isolated isoquercitrin showed cytotoxicity against SiHa cell line at IC50 of 980 μM. Messenger RNA (mRNA) expression of TRAIL-Rs was quantified by qRT-PCR, combination of isoquercitrin, and/or rhTRAIL increased TRAIL-R1 and TRAIL-R2 gene expression by 7 folds and 4 folds, respectively. Also, FACS assay revealed that combined treatment has increased the early apoptosis up to 7.24%. In the present study, we found that isoquercitrin enhances the mRNA expression of TRAIL-Rs, but the percentage of apoptosis was meager, possibly due to the influence of other anti-apoptotic proteins.

  13. Bacterias marinas productoras de compuestos antibacterianos aisladas a partir de invertebrados intermareales Marine bacteria producing antibacterial compounds Isolated from inter-tidal invertebrates

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    Jorge León

    2010-06-01

    Full Text Available Se realizó actividades prospectivas de muestreo de invertebrados intermareales en la Bahía de Ancón (Lima - Perú con el objetivo de seleccionar bacterias marinas productoras de sustancias antimicrobianas. El estudio comprendió el aislamiento de bacterias en agar marino, pruebas de susceptibilidad antimicrobiana in vitro y observaciones de microscopía electrónica. Se reporta el aislamiento, caracterización fenotípica y propiedades antimicrobianas de diez cepas de bacterias marinas que incluyen a los géneros Vibrio, Pseudomonas y Flavobacterium y del orden Actinomycetal que inhiben a patógenos de humanos. Los resultados indicarían que los invertebrados marinos serían fuentes de bacterias productoras de sustancias antibióticas.Prospective sampling activities of intertidal invertebrates in the Ancon Bay (Lima, Peru were done in order to select marine bacteria producing antimicrobial substances. The study included the isolation of bacteria in marine agar, in vitro antimicrobial susceptibility testing and electronic microscopic observations. We report the isolation, phenotypical characterization and antimicrobial properties of 10 strains of marine bacteria including the genus Vibrio, Pseudomonas, and Flavobacterium, and the order Actinomycetae that inhibit human pathogens. The results indicate that the marine invertebrates would be sources of bacteria producing antibiotic substances.

  14. Evaluación biológica de una fracción de la esponja marina Topsentia ophiraphidites del Caribe colombiano

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    Lina Blandón G.

    2013-10-01

    Full Text Available Objetivo. Evaluar la actividad antiproliferativa y genotóxica de una fracción con actividad citotóxica obtenida de la esponja marina del Caribe colombiano Topsentia ophiraphidites (Fracción T4. Materiales y métodos. La fracción T4 de la esponja marina Topsentia ophiraphidites fue obtenida en el laboratorio de Productos Naturales Marinos de la Universidad de Antioquia. La actividad antiproliferativa se evaluó mediante ensayos de eficiencia de clonación, función de acumulación y cinética proliferativa por intercambio de cromátidas hermanas (ICH; la actividad genotóxica se evaluó mediante electroforesis en gel de células individuales (Ensayo cometa e intercambio de cromátidas hermanas (ICH. Todas las pruebas fueron realizadas sobre las líneas celulares Jurkat y CHO. Resultados. La fracción T4 afectó el ciclo celular de las células CHO y mostró daño genotóxica crónico en las células Jurkat. Conclusiones. Se recomienda la evaluación de la fracción T4 en otras líneas celulares derivadas de tumor con el fin de determinar un posible efecto diferencial, además de evaluar otras actividades de tipo antimicrobiano, antimalárico, entre otros.

  15. Red de Repositorios IODE/COI/Unesco de acceso abierto en Ciencias Marinas en América Latina y el Caribe: OceanDocs

    Directory of Open Access Journals (Sweden)

    Andrea Cristiani

    2014-03-01

    Full Text Available Se presenta la organización, funciones y evolución de la red de repositorios de acceso abierto OceanDocs, particularmente la experiencia de instituciones vinculadas a las Ciencias Marinas en América Latina y el Caribe. En el marco de las Redes de Datos e Información Oceanográfica de la Comisión Oceanográfica Intergubernamental (COI de UNESCO, surgieron iniciativas de repositorios locales y regionales, que más tarde se consolidan y dan lugar a la red internacional de repositorios en Ciencias Marinas:  OceanDocs. En la actualidad esta red está integrada por repositorios institucionales, repositorios regionales y un repositorio central alojado por la COI. OceanDocs cuenta con políticas comunes definidas, un Grupo Directivo conformado por especialistas de distintas áreas que planifica y establece las directrices para la red, coordinadores nacionales y regionales, Unidades de Información a nivel institucional responsables de la carga de documentos, y una creciente comunidad de usuarios a nivel global.

  16. Optimización de recursos hídricos y armonización de sus usos: el Consorcio de Aguas de la Marina Baja

    Directory of Open Access Journals (Sweden)

    Antonio Gil Olcina

    2010-01-01

    Full Text Available En la Marina Baja, comarca alicantina de la seca región climática del Sureste Ibérico, el difícil desafío de conjugar el extraordinario desarrollo turístico y urbano de su franja litoral, que polariza Benidorm, con disponibilidades hídricas exiguas e irregulares, ha requerido un conjunto de actuaciones del mayor interés, para la optimización de éstas y la armonización de sus usos, protagonizadas por el Consorcio de Aguas de la Marina Baja. Dichas iniciativas incluyen: mayor y mejor regulación de recursos epigeos, potenciación sostenible de los subterráneos, y reutilización de aguas depuradas, con superposición de dos sistemas independientes -aguas blancas y depuradas-, que funcionan al unísono; sin que falte, para conjurar el riesgo extremo de una sequía prolongada e intensa, la posibilidad de emplear caudales foráneos.

  17. Distribution patterns of Syllidae (Annelida: Polychaeta from seagrass (Zostera marina and Z. noltei meadows in the Ensenada de O Grove (Galicia, NW Spain

    Directory of Open Access Journals (Sweden)

    Patricia Quintas

    2013-09-01

    Full Text Available This paper describes the distribution and composition of the syllid fauna inhabiting seagrass meadows in the Ensenada de O Grove (NW Spain. Samples were collected on muddy sediments colonized by either Zostera marina L., Zostera noltei Hornemann or by a mixed meadow with both species. Syllids were dominant (13340 individuals; 37% of total polychaete abundance, including 22 species (12 genera. The mixed meadows housed the highest number of species and the Z. noltei meadow had practically no syllids. The dominant species were Exogone naidina, Parapionosyllis elegans, Parexogone hebes and Prosphaerosyllis campoyi ( > 80% of total abundance. Carnivores (mainly species of Parapionosyllis, Amblyosyllis, and Streptosyllis were dominant, especially in muddy sand with either Z. marina or Z. noltei and sandy mud with a mixed meadow. The most important abiotic variables for explaining the composition and distribution of the syllid fauna were bottom water salinity, sorting coefficient and carbonate content. The highest number of species was recorded at sites with a high salinity and carbonate content and the lowest at sites with a high sorting coefficient.

  18. Characterisation of sulphide-bearing waste-rock dumps using electrical resistivity imaging: the case study of the Rio Marina mining district (Elba Island, Italy).

    Science.gov (United States)

    Mele, Mauro; Servida, Diego; Lupis, Domenico

    2013-07-01

    Sulphide-bearing mine dumps are potential sources of pollution when acid mine drainage (AMD) occurs. Because the generation of AMD depends on the volume and composition of waste materials, their characterisation is crucial for the evaluation of geochemical hazards and for the design of remediation strategies to minimise their environmental impact. In this paper, a cost-effective strategy for the characterisation of an inactive mine dump in the Rio Marina mining district (Elba Island, Italy) using earth resistivity imaging (ERI) is presented. As no information regarding the nature of waste rocks is found in reports for the mine, five ERI profiles were acquired at the top of the waste pile. The results show that waste rocks are heterogeneous with a maximum thickness of 30 m. Due to the large amounts of dispersed sulphide minerals, the waste rocks are characterised by an electrically conductive geophysical signature in comparison to the surrounding resistive metamorphic bedrock. A geostatistical approach was adopted to estimate the elevation of the edges of the mine dump, and the net volume of the waste rocks was computed through a raster analysis of the elevations of the upper and lower boundaries of the mine dump. High-conductivity anomalies were detected within the core of the mine dump. The integration of the hydrogeological, geochemical and geological framework of the Rio Marina mining district suggests that these anomalies could be a geophysical signature of subsurface regions where AMD is currently generated or stored, thus representing sources of environmental pollution.

  19. Predicting the functionally distinct residues in the heme, cation, and substrate-binding sites of peroxidase from stress-tolerant mangrove specie, Avicennia marina.

    Science.gov (United States)

    Jabeen, Uzma; Abbasi, Atiya; Salim, Asmat

    2011-11-01

    Recent work was conducted to predict the structure of functionally distinct regions of Avicennia marina peroxidase (AP) by using the structural coordinates of barley grains peroxidase as the template. This enzyme is utilized by all living organisms in many biosynthetic or degradable processes and in defense against oxidative stress. The homology model showed some distinct structural changes in the heme, calcium, and substrate-binding regions. Val53 was found to be an important coordinating residue between distal calcium ion and the distal heme site while Ser176 is coordinated to the proximal histidine through Ala174 and Leu172. Different ionic and hydrogen-bonded interactions were also observed in AP. Analyses of various substrate-enzyme interactions revealed that the substrate-binding pocket is provided by the residues, His41, Phe70, Gly71, Asp138, His139, and Lys176; the later three residues are not conserved in the peroxidase family. We have also performed structural comparison of the A. marina peroxidase with that of two class III salt-sensitive species, peanut and soybean. Four loop regions were found to have largest structural deviation. The overall protein sequence was also analyzed for the presence of probable post-translational modification sites and the functional significance of these sites were outlined.

  20. An economical non-destructive method for estimating eelgrass, Zostera marina (Potamogetonaceae) leaf growth rates: formal development and use in northwestern Baja California.

    Science.gov (United States)

    Solana-Arellano, Elena; Echavarria-Heras, Héctor; Franco-Vizcaíno, Ernesto

    2008-09-01

    Seagrass beds provide much of the primary production in estuaries; host many fishes and fish larvae, and abate erosion. The present study presents original analytical methods for estimating mean leaf-growth rates of eelgrass (Zostera marina). The method was calibrated by using data collected in a Z. marina meadow at Punta Banda estuary in Baja California, Mexico. The analytical assessments were based on measurements of leaf length and standard regression procedures. We present a detailed explanation of the formal procedures involved in the derivation of these analytical methods. The measured daily leaf-growth rate was 10.9 mm d(-1) leaf(-1). The corresponding value projected by our method was 10.2 mm d(-1) leaf(-). The associated standard errors were of 0.53 and 0.56 mm d(-1) leaf(-1) respectively. The method was validated by projecting leaf-growth rates from an independent data set, which gave consistent results. The use of the method to obtain the mean leaf growth rate of a transplanted plot is also illustrated. Comparison of our leaf-growth data with previously reported assessments show the significant forcing of sea-surface temperature on eelgrass leaf dynamics. The formal constructs provided here are of general scope and can be applied to equivalent eelgrass data sets in a straightforward manner.

  1. Presencia de bacterias asociadas a nieve marina en arrecifes influenciados por escorrentía continental del Caribe colombiano: primer acercamiento

    Directory of Open Access Journals (Sweden)

    Hernán Alejandro Henao-Castro

    2015-06-01

    Full Text Available Parte del deterioro de los arrecifes de coral se atribuye al efecto del aporte de agua dulce cargada de nutrientes y sedimentos finos. Estas condiciones favorecen la formación de nieve marina lodosa que transporta gran carga de bacterias que pueden ser patógenos de corales. Por lo anterior, se estudió la presencia de bacterias asociadas a nieve marina lodosa en una zona arrecifal del Caribe colombiano en un gradiente de distancia a la desembocadura del canal del Dique (Cartagena. Por medio de técnicas moleculares PCR-DGGE, se registró baja riqueza, con bandas representativas de siete bacterias diferentes. El número de especies varió según el sitio, pero no hubo relación con la cercanía a la fuente de escorrentía. Como estudio pionero, sirve como punto de partida para estudios en arrecifes del Caribe colombiano impactados por escorrentía.

  2. Identification and characterization of a chitin-binding protein purified from coelomic fluid of the lugworm Arenicola marina defining a novel protein sequence family

    DEFF Research Database (Denmark)

    Vitashenkova, Nina; Moeller, Jesper Bonnet; Leth-Larsen, Rikke

    2012-01-01

    , respectively, were isolated from a coelomocyte cDNA library. The two clones designated AML-1a and AML-1b were 92% identical at the protein level, and represent a novel type of protein sequence family. Purified AML-1 induced agglutination of rabbit erythrocytes, which could be inhibited by N......-acetylated saccharides. Recombinant AML-1b showed the same band pattern as the native protein, whereas recombinant AML-1a reduced lacked a 27-kDa band. AML-1b bound GlcNAc-derivatized columns and chitin, whereas AML-1a did not bind to these matrices. Immunohistochemical analysis revealed, that AML-1 is expressed......We have isolated a novel type of lectin named Arenicola marina lectin-1 (AML-1) from the lugworm Arenicola marina. The lectin was purified from the coelomic fluid by affinity chromatography on a GlcNAc-derivatized column and eluted with GlcNAc. On SDS-PAGE, AML-1 showed an apparent molecular mass...

  3. Autophagy Inhibition Enhances the Mitochondrial-Mediated Apoptosis Induced by Mangrove (Avicennia marina) Extract in Human Breast Cancer Cells

    KAUST Repository

    Esau, Luke

    2015-01-10

    Aims: Avicennia marina (AM) is a widely distributed mangrove plant that has been used in traditional medicine for centuries for the treatment of a number of diseases. The objective of the present study was to evaluate the leaf ethyl acetate extract of AM for its cytotoxic and apoptotic potential along with in-depth investigations of its mechanism of action in breast cancer MCF-7 cells. Study Design: The ethyl acetate extract of leaves and stems of AM was tested against estrogen positive breast cancer cell line MCF-7 using various assays. Place and Duration of Study: The study was carried out at King Abdullah University of Science and Technology, Thuwal, Saudi Arabia, from July 2013-June 2014. Methodology: Dose- and time-dependent growth inhibition of cancer cells was measured using MTT assay. The mechanisms of apoptosis induction were determined using various assays: phosphatidylserine exposure, caspase-3/7 activation, mitochondrial membrane potential disruption, reactive oxygen species (ROS) production, cell cycle analysis, autophagy, and protein expression using western blotting. The modulation of apoptotic genes (p53, Mdm2, NF-kB, Bad, Bax, Bcl-2 and Casp7) was also determined using real time PCR. Results: The AM extract inhibited breast cancer cell growth and induced apoptosis in a concentration dependent manner. We demonstrated a non-classical mode of apoptosis induction in MCF-7 cells by AM extract, where ROS production altered the mitochondrial membrane potential to induce apoptosis. Breast cancer cells treated with 200 µg/ml concentration of AM extract showed increased ROS production and disrupted MMP but no PARP-1 cleavage and a marked decrease in Caspase-7 protein levels (24 and 48 h) were detected. A significant amount of autophagy was also observed at the same concentration. However, treatment of MCF-7 cells with 200 µg/ml of AM extract along with the inhibition of autophagy by chloroquine, significantly increased the apoptosis from 20% to 45

  4. COMPOSICIÓN QUIMICA Y ACTIVIDAD ANTIFOULING DE LA FRACCION LIPIDICA DE LA ESPONJA MARINA Cliona tenuis (Clionidae

    Directory of Open Access Journals (Sweden)

    Leonardo Castellanos

    2009-04-01

    Full Text Available Normal 0 21 false false false st1\\:*{behavior:url(#ieooui } /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Tabla normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Times New Roman"; mso-ansi-language:#0400; mso-fareast-language:#0400; mso-bidi-language:#0400;} Del extracto orgánico de la esponja marina Cliona tenuis, recolectada en las Islas del Rosario (Colombia, Mar Caribe, fue obtenida la fracción lipídica, la cual presentó propiedades antifouling en pruebas en campo. Esta fracción fue separada por CC sobre gel de sílice hasta obtener fracciones de ésteres metílicos, glicéridos, glicolípidos, fosfolípidos y ácidos grasos libres, las cuales fueron identificadas por CCD y técnicas de dereplicación (RMN 1H y 13C. Posteriormente, las fracciones de glicéridos, glicolípidos y fosfolípidos fueron hidrolizadas y los ácidos obtenidos, junto con los provenientes de la fracción de ácidos grasos libres, fueron transformados en ésteres metílicos y todos se analizaron por CGAR-EM. Para ubicar las insaturaciones y ramificaciones, los ésteres metílicos se transformaron luego en sus correspondientes pirrolididas, las cuales también se analizaron por CGAR-EM. El estudio cromatográfico (valores de ECL y de los espectros de masas de los ésteres metílicos y de sus derivados pirrolididas permitió identificar 81 ácidos grasos diferentes, de los cuales no habían sido previamente reportados: los ácidos 4,8-hexadecadienoico, 11-metil-4,10-octadecadienoico, 6,9,12,14-icosatetraenoico, y 6,9,12,14,17-icosapentanoico.

  5. Comparación ontogénica de la frecuencia de muda en Rhinella marina (Anura, Bufonidae

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    Teófila M. Triana

    2013-03-01

    Full Text Available La muda es un proceso de renovación de la capa externa de la epidermis (estrato corneo de los anfibios, la cual suministra protección contra daños, patógenos y pérdida de agua. Este trabajo evalúa la frecuencia de muda entre juveniles y adultos de Rhinella marina (Linnaeus, 1758 y la tasa de ocurrencia entre el día y la noche. Para esto, se realizaron dos observaciones diarias (7 am y 7 pm, entre Octubre de 2011 y Marzo de 2012, a tres grupos de individuos clasificados según su tamaño longitud rostro-cloaca, como adultos (promedio=80 mm, juveniles medianos (promedio=19 mm y juveniles pequeños (promedio=13 mm. Estos animales fueron colocados en terrarios en el laboratorio y marcados en el dorso a través de un punto hecho con un corrector de tinta. La muda se determinó por la pérdida total de la marca y una coloración brillante en el dorso. Se encontró una diferencia significativa (Kruskal-Wallis, H=19.84, p<0.0001 en el periodo de muda entre los tres grupos de estudio: adultos=7.5 días, juveniles medianos=5.4 días, juveniles pequeños=5.3 días. También, en la frecuencia de animales que mudaron en el día y la noche (Ji-cuadrado, χ2=7.891, p=0.019, particularmente en los dos grupos de juveniles, quienes lo hicieron en la noche, ya que en los adultos no hubo una diferencia clara. Posiblemente, la mayor frecuencia de la muda en los juveniles puede relacionarse con su condición ontogénica, de un menor tamaño corporal, alta tasa metabólica y mayor tasa de desarrollo.

  6. Reproductive and population parameters of Thais chocolata (Duclos, 1832) (Gastropoda, Thaididae) in La Rinconada marine reserve, Antofagasta, Chile/ Parametros reproductivos y poblacionales de Thais chocolata (Duclos, 1832) (Gastropoda, Thaididae), en la reserva marina La Rinconada, Antofagasta, Chile

    National Research Council Canada - National Science Library

    Cantillanez, Marcela; Avendano, Miguel; Rojo, Manuel; Olivares, Alberto

    2011-01-01

    ... Aulacomya ater, Tagelus dombeii, y el caracol carnivoro Thais chocolata (Ortiz et al., 2009a). Estudios recientes han demostrado que el area de la reserva marina que ocupa una superficie de 350 ha, esta sujeta a flujos alternados de corriente que resultan en una corriente predominante dirigida hacia el norte, pero que se encuentra con la barrera impues...

  7. Modelado Energético de Convertidores Primarios para el Aprovechamiento de las Energías Renovables Marinas

    Directory of Open Access Journals (Sweden)

    Amable López

    2014-04-01

    Full Text Available Resumen: El objetivo principal de este artículo es presentar los métodos más habituales de aprovechamiento de distintos tipos de energías renovables procedentes del mar y analizar los modelos energéticos de los dispositivos utilizados para su explotación. Estos modelos son necesarios para el diseño del dispositivo así como para el estudio de su comportamiento dinámico. Su conocimiento resulta imprescindible también para su simulación dinámica y para el diseño de los algoritmos de control necesarios para conseguir una optimización energética y económica. En este trabajo se presentan algunos de los diferentes tipos de energías del mar, y se j ustifica el interés en el desarrollo de dispositivos específicos para el aprovechamiento de las corrientes marinas y de las olas, junto con una pequeña clasificación en función de la profundidad del agua en la zona de instalación de estos dispositivos. Para los convertidores de energía de las olas, generalmente, de tipo resonante, se presenta una metodología de tipo general, analizando los distintos campos de fuerzas actuantes y los métodos de obtención de las respuestas temporal y frecuencial. Para los dispositivos de aprovechamiento de las corrientes, se propone una metodología simplificada de modelado dinámico que puede ser utilizada en análisis del dispositivo concreto. Para ello se tiene en cuenta los perfiles hidrodinámicos que utilizan estos dispositivos, los datos del perfil de la corriente con la profundidad y del modelo de oleaje, y la dinámica propia del conjunto multiplicadora y generador eléctrico. La metodología propuesta se aplica al estudio de un caso concreto, correspondiente a un rotor de eje horizontal de un convertidor de energía de las corrientes. Los resultados obtenidos permiten analizar los efectos del oleaje y de las condiciones de flujo de la corriente no uniforme sobre las magnitudes energéticas más importantes. Abstract: The main objective of

  8. Trama trófica de una comunidad de peces en una pradera marina en el Caribe Venezolano

    Directory of Open Access Journals (Sweden)

    Adriana López-Ordaz

    2009-12-01

    Full Text Available Las praderas marinas son consideradas sitios importantes de alimentación para una variedad de especies de peces, por lo que en estos sistemas son frecuentes las interacciones tróficas complejas. En el presente trabajo se describió la trama trófica de la ictiofauna en una pradera marina ubicada en el Caribe Venezolano. Adicionalmente, se propuso una medida de la intensidad de consumo de cada presa (IC, considerando dos variables: la abundancia y la frecuencia de uso. Se realizaron ocho muestreos (en 4 horarios: 6:00, 12:00, 18:00 y 24:00 horas en agosto 2005 y enero 2006. Se capturaron 51 especies pertenecientes a 29 familias siendo Haemulidae la más abundante y se identificaron 28 items alimenticios dentro de los cuales, Crustácea fue el más importante como recurso alimenticio. La importancia de los gremios tróficos considerando la abundancia y la riqueza taxonómica de especies fue: bentófago>herbívoro>piscívoro. Las características de la trama trófica fueron las siguientes: 1 una mayor proporción de cadenas cortas (dos eslabones, 2 un elevado número de especies intermedias. 3 un elevado consumo de bentos, 4 unas bajas intensidades de consumo y 5 un reducido número de especies involucradas en el mayor número de uniones tróficas estimadas. Se obtuvieron diferencias temporales (horarias y mensuales en el número de especies (totales, intermedias y topes, de uniones totales, en la conectividad y en la longitud máxima de cadena. No se observó una variación temporal en la conectancia y sus valores resultaron parecidos a los reportados para otros ambientes marinos con distinta riqueza de especies. Por el contrario, los valores de conectividad resultaron menores a los obtenidos en ambientes con menor riqueza de especies. Como en otros reportes, los parámetros tróficos estimados fueron poco sensibles a los cambios intracomunitarios. IC resultó bajo, a excepción de lo obtenido para los copépodos harpacticoides y las larvas de

  9. Escenarios relacionados con la intrusión marina. Caso de estudio Cuenca Artemisa – Quivicán, Provincia Habana. La Habana – Cuba Escenarios relacionados con la intrusión marina. Caso de estudio Cuenca Artemisa – Quivicán, Provincia Habana. La Habana – Cuba

    Directory of Open Access Journals (Sweden)

    Manuel Núñez Lafitte

    2008-12-01

    Full Text Available Sea water intrusion is the process by which the sea water in an aquifer displaces the sweet water as consequence of a sensitive reduction of the hydraulic head, either for natural actions or for anthropic activity Sea water intrusion takes place in the coastal aquifers mainly independently to the predominant lithology. In this work the scenario analysis is approached by studying the effects that sea water intrusion in dry years would produce to the supply of underground water to the City of Havana and Havana Province, when there would be high exploitation of the aquifers without maintenance of recharge works such as in South Dike.There would be a cartography of the scenario in danger to show the presage of the penetration expressed in the longitude of the marine intrusion wedge, taking as contour conditions the variable that may be unfavorable for their temporal variation, such as precipitation and depression. The thickness of the sheet of sweet water is considered constant.La intrusión marina es el proceso por el cual el agua de mar desplaza en un acuífero el agua dulce como consecuencia de una reducción sensible de la carga hidráulica, ya sea por acciones naturales o por actividad antrópica.La intrusión marina tiene lugar principalmente en los acuíferos costeros independientemente a la litología predominante.En este trabajo se aborda el análisis del escenario que en el abasto de agua subterránea a la Ciudad de La Habana y Provincia Habana produciría la intrusión marina durante la ocurrencias de años secos, simultaneados, con altas explotaciones del acuífero y el no mantenimiento de obras de recarga como es el caso del Dique Sur.Se cartografía el escenario de peligro que muestra el pronóstico de la penetración expresada en longitud de la cuña de intrusión marina tomando como condiciones de contorno las variables que pueden ser desfavorables por su variación temporal, tales como la precipitación, explotación y abatimiento

  10. El alga marina Sargassum (Sargassaceae: una alternativa tropical para la alimentación de ganado caprino

    Directory of Open Access Journals (Sweden)

    M Casas-Valdez

    2006-03-01

    Full Text Available Se estudió el valor nutricional de varias algas del género Sargassum. Veinte cabras hembras (Nubia en crecimiento (43 semanasse distribuyeron aleatoriamente en dos grupos de 10 cabras y se alojaron en corraletas individuales. Un grupo fue alimentado con una dieta testigo elaborada con insumos regionales y el otro con una dieta que incluyó el 25% de Sargassum spp. Durante 60 días se midieron diariamente y en forma individual, el alimento y agua consumidos, el peso corporal se cuantificó cada 15 días. La composición química proximal de Sargassum fue de: 89% materia seca, 7.7% proteína, 31% cenizas, 2% extracto etéreo y 39% de carbohidratos. También se determinaron las fracciones de fibra, minerales, vitaminas, ácidos grasos y factores antinutricios. No se encontraron diferencias significativas en el peso corporal (8.6 kg grupo control y 9 kg experimental, alimento consumido (1.31 kg grupo control y 1.6 experimentaly tasa de conversión alimenticia (11.1 grupo control y 12.6 experimental entre los tratamientos. El consumo de agua fue mayor en el grupo alimentado con la dieta que incluyó Sargassum spp (5.1 l. De los resultados se deduce que las algas marinas de este género pueden ser utilizadas como un forraje alternativo de mediana calidad para el ganado caprino.The seaweed Sargassum (Sargassaceae as tropical alternative for goats’ feeding. The nutritive value of seaweed (Sargassum spp. was studied in Baja California Sur, Mexico. Twenty female Nubian goats (43-weeks old were randomly distributed into two groups of 10 goats each and were housed in individual pens. One group was fed with a control diet and the other with a diet supplemented with 25% of Sargassum spp. Feed and water intake were recorded daily and individually for 60 days. The weight of each goat was recorded every 15 days. The nutritional content of Sargassum spp. was 89% dry mater, 8% crude protein, 31% ash, 2% ether extract, and 39% carbohydrates. Fiber fractions

  11. La familia Hemiaulaceae (Bacillariophyceae de las aguas marinas chilenas The family Hemiaulaceae (Bacillariophyceae from marine Chilean waters

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    PATRICIO RIVERA

    2003-12-01

    Full Text Available La familia Hemiaulaceae comprende cuatro géneros, señalados previamente para las aguas chilenas con las siguientes especies: Cerataulina pelagica (Cleve Hendey, Hemiaulus sinensis Greville, H. membranaceus Cleve, Climacodium biconcavum Cleve, Eucampia antarctica (Castracane Mangin, E. cornuta (Cleve Grunow y E. zodiacus Ehrenberg. Sin embargo, el análisis con microscopía fotónica y electrónica de muestras recolectadas en aguas marinas chilenas que contenían representantes de esta familia (incluyendo la mayoría de aquellas estudiadas anteriormente por otros investigadores nacionales reveló que (1 el género Cerataulina está representado por C. pelagica, distribuida a lo largo de la costa chilena entre Arica por el norte y el Estrecho de Magallanes por el sur, (2 el género Eucampia está representado por cuatro taxa: E. zodiacus f. cylindrocornis Syvertsen (señalada en trabajos anteriores como E. zodiacus f. zodiacus, E. zodiacus f. recta Rivera. Avaria & Cruces f. nov. (descrita aquí, E. cornuta y E. antarctica. Los primeros tres taxa se distribuyen en la zona central y norte de Chile, mientras que E. antarctica es propia de las aguas antárticas desde el Estrecho de Magallanes al sur, (3 las citas anteriores de Hemiaulus sinensis, H. membranaceus y Climacodium biconcavum para las aguas chilenas corresponden a determinaciones erróneas de Eucampia zodiacus f. recta, f. nov. Se entregan descripciones de los taxa encontrados y fotografías obtenidas con los microscopios fotónico y electrónicos que ilustran sus principales características morfológicasThe Family Hemiaulaceae comprises four genera, all of which have been previously reported for coastal waters off Chile, and represented by the following taxa: Cerataulina pelagica (Cleve Hendey, Hemiaulus sinensis Greville, H. membranaceus Cleve, Climacodium biconcavum Cleve, Eucampia antarctica (Castracane Mangin, E. cornuta (Cleve Grunow and E. zodiacus Ehrenberg. However, examination

  12. Revisión zoogeográfica marina del sur del Brasil A marine zoogeographical revision of southern Brazil

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    Francisco J Palacio

    1982-01-01

    Full Text Available El estúdio inicial de la fauna y distribución de cefalópodos de Brasil se convirtio en una revisión de la zoogeografía de la región Tropical-Subtropical del sur del Brasil. Observaciones iniciales sobre zoogeografía marina brasileña tuvieron lugar en la primera parte del siglo XIX por naturalistas franceses, principalmente d'Orbigny, trabajando en cefalópodos. Posteriormente, el primer patrón global de provincias zoologicas, definidas por James Dana en 1853 y basadas en zonas de temperaturas oceanicas, colocan el extremo meridional de la fauna tropical del Atlántico Occidental en una latitud de 30ºS, incluyendo una zona de transición (São Paulo entre Rio de Janeiro y Porto Alegre. El análisis detallado de numerosos estúdios subsecuentes en esta región lleva a la confirmación de la validez conceptual de los puntos de vista de d'Orbigny, Dana, Forbes, Woodward y algunos mas durante el siglo pasado. En su tratado zoogeográfico de 1953, Ekman no tuvo a su disposición información adecuada sobre Brasil y señaló vagamente a Rio de Janeiro como el extremo sur de la fauna tropical, basado en la aparente ausencia de manglares y de arrecifes de coral. Este punto de vista ha sido fuente de confusión y debate, especialmente en el siglo XX. Toda la información oceanógrafica, geológica, sedimentaria y faunística acumulada y estudiada hasta el presente señala a la región entre Rio Grande do Sul y Rio de la Plata, como la barrera ecológica fundamental (principalmente debido a cambios de temperatura, en la distribución sur de organismos tropicales. Entre Espirito Santo y Rio Grande do Sul existe una zona de transición faunística caracterizada por complejas variaciones medioambientales estacionales, bajo la influencia alterna y combina de las Corrientes del Brasil y Falkland, en la margen occindetal de la Convergencia Subtropical/Subantartica. Esta zona de transición constituye una provincia biogeografica, designada "Paulista

  13. Crecimiento y estructura demográfica de Argopecten purpuratus en la Reserva Marina La Rinconada, Antofagasta, Chile

    Directory of Open Access Journals (Sweden)

    2005-01-01

    Full Text Available Se presentan los parámetros de crecimiento L∞ = 120.38 mm y K = 0.9668 para el banco de Argopecten purpuratus de la Reserva Marina La Rinconada (Antofagasta, Chile, obtenidos entre marzo de 2001 y mayo de 2003 usando métodos de marcaje y recaptura de ejemplares. La elevada recaptura de individuos marcados (60–80% indica baja mortalidad natural y una conducta sedentaria del recurso. Las evaluaciones anuales realizadas para estimar el tamaño del banco confirmaron tal conducta, encontrándose la mayoría de los individuos agregados en el centro, con densidades que variaron entre años de 9 a 14.8 ind m–2. La abundancia de ejemplares también mostró variabilidad interanual, con cifras de 10.1 × 106 en 2001, 8.2 × 106 en 2002 y 12.4 × 106 en 2003. Estas evaluaciones mostraron, además, una disminución en la talla media de la estructura demográfica del banco entre un año y otro, reduciéndose de 75.8 mm en 2001 a 62.1 mm en 2002 y a 51.7 mm en 2003. Esta disminución también ocurrió con el número de ejemplares mayores o iguales a la talla mínima legal (90 mm, reduciéndose de 2.6 × 106 ejemplares presentes en 2001 a 3.7 × 105 ejemplares en 2003. La aplicación de los parámetros de crecimiento sobre la estructura poblacional del banco entre 2002 y 2003 señaló la integración de una nueva clase anual de 6.5 × 106 de ejemplares en 2002 y de 11.2 × 106 de individuos en 2003. El descuento de estas cifras, del número total de ejemplares presentes en ambos años, demuestra una pérdida cercana a los 8.4 × 106 de ostiones en 2002 y de cerca de 7.0 × 106 de individuos en 2003. Estas pérdidas correspondieron a ejemplares con tallas superiores a los 60 mm, lo cual es corroborado con el análisis de la curva de captura realizado con el programa Fisat, cuyos resultados señalan una mortalidad Z = 2.15 en 2002 para el segmento de la población entre 67.5 y 115.5 mm, y Z = 3.08 en 2003 para el segmento de 64.3 a 112.5 mm. Los

  14. Wild cane toads (Rhinella marina expel foreign matter from the coelom via the urinary bladder in response to internal injury, endoparasites and disease.

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    Crystal Kelehear

    Full Text Available Dissections of >1,200 wild-caught cane toads (Rhinella marina in tropical Australia confirm a laboratory report that anurans can expel foreign objects from the coelom by incorporating them into the urinary bladder. The foreign objects that we found inside bladders included a diverse array of items (e.g., grass seeds, twigs, insect prey, parasites, many of which may have entered the coelom via rupture of the gut wall. In some cases, the urinary bladder was fused to other organs including liver, fat bodies, ovaries, Bidder's organs, lungs, mesentery, stomach wall, gall bladder, and the abdominal wall. Acanthocephalan parasites (of a range of developmental stages were identified from the walls of the urinary bladders of three cane toads. This organ may play a significant role in destroying or excreting metazoan parasites, as well as inanimate objects.

  15. Wild cane toads (Rhinella marina) expel foreign matter from the coelom via the urinary bladder in response to internal injury, endoparasites and disease.

    Science.gov (United States)

    Kelehear, Crystal; Jones, Hugh I; Wood, Benjamin A; Shine, Richard

    2015-01-01

    Dissections of >1,200 wild-caught cane toads (Rhinella marina) in tropical Australia confirm a laboratory report that anurans can expel foreign objects from the coelom by incorporating them into the urinary bladder. The foreign objects that we found inside bladders included a diverse array of items (e.g., grass seeds, twigs, insect prey, parasites), many of which may have entered the coelom via rupture of the gut wall. In some cases, the urinary bladder was fused to other organs including liver, fat bodies, ovaries, Bidder's organs, lungs, mesentery, stomach wall, gall bladder, and the abdominal wall. Acanthocephalan parasites (of a range of developmental stages) were identified from the walls of the urinary bladders of three cane toads. This organ may play a significant role in destroying or excreting metazoan parasites, as well as inanimate objects.

  16. »Halfway Tradition«: Transition, Nation, Sex, and Death in the Work of Marina Abramović and Mladen Miljanović

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    Uroš Čvoro

    2017-09-01

    Full Text Available This article examines how the artists Marina Abramović and Mladen Miljanović apprehend the terms Balkan and Europe as frameworks for understanding the post-communist transition in the countries of the former Yugoslavia. Abramović’s representations of pagan sex rituals in Balkan Erotic Epic (2005 and tombstone engravings in Miljanović’s The Garden of Earthly Delights (2013 capture what I describe as “halfway traditions”: cultural practices that simultaneously problematize the normative teleology of the Balkans moving away from primitivism and toward the civilization of Europe, and act as parodies of the nationalist reinvention of tradition. By highlighting “halfway tradition” as the symbol of the post-communist transitional state and a disruptive by-product of transition, Abramović and Miljanović critique ethnonationalist politics of death and sex, and articulate an “in-between” temporality that disrupts the teleology of transition.

  17. Screening of Salt-stress, Antioxidant Enzyme, and Antimicrobial Activity of Leave extracts of Mangroves Avicennia marina L. from Hodaidah, Yemen

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    Maher A. Al Maqtari

    2014-05-01

    Full Text Available In the present study the salinity stress, antioxidant enzyme and antimicrobial activities of leaf extract of Avicennia marinawere investigated. As visualized from SDS-PAGE, no differences was found in number of protein band, but the intensities of several protein bands having apparent molecular mass by reduced severely in salt treated samples with enhanced activities of CAT, POX and GPX. Escherichia coli (ATCC8739, Staphylococus aureus (ATCC 6538, and Bacillus subtilis (ATCC6633 and fungus (Candida albicans ATCC 2091, and Aspergillus niger ATCC 16404 were used as the test pathogenic bacteria and fungi, respectively in this study. The Avicennia marina extract possessed antibacterial activity against E. coli, S. aureus, and B. subtilis (12, 6, and 7 mm respectively, with antifungal activity against C. albicans and A. niger (9 and 10 mm.

  18. Marinas e náutica no litoral brasileiro: aportes metodológicos introdutórios para a pesquisa e o planejamento territorial do turismo.

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    Daniel Hauer Queiroz Telles

    2013-01-01

    Full Text Available In this article it is analyzed the concept of territory from non-exclusive conceptions with the intention to understand the marines along the coast of Brazil. The aim is to contribute to the generation of scientific and technological knowledge on the subject, presenting a definition, a classification and a genesis, as well as, highlighting its social relevance. It presents a summarized literature review on the matter and stipulates the cross-scale perspective supported by the approaches that analyze the phenomenon in question. The approaches are based on the productive dimension and the identification of the urban environment; show the importance and necessity of new proposals on the subject matter. Classification and genesis of marinas in Brazil are presented in stages, it is emphasized the importance of tourism in territorial planning to act and narrow the gaps between knowledge and politics; whereas the nautical sector is no subject of discussion in the coastal zone of Brazil.

  19. Assessment of heavy metal and petroleum hydrocarbon contamination in the Sultanate of Oman with emphasis on harbours, marinas, terminals and ports.

    Science.gov (United States)

    Jupp, Barry P; Fowler, Scott W; Dobretsov, Sergey; van der Wiele, Henk; Al-Ghafri, Ahmed

    2017-08-15

    The assessment here includes data on levels of contaminants (petroleum hydrocarbons and heavy metals) in sediments and biomonitor organisms, including the eulittoral rock oyster Saccostrea cucullata and subtidal biomonitors, the barnacle Balanus trigonus and the antipatharian coral Antipathes sp., at harbours, marinas, terminals and large ports along the coastline of Oman. TBT levels in harbour and port sediments up to a maximum of 100ppb TBT dry weight are highlighted. Oysters contained concentrations up to 367ppm mg TPH/kg dry weight. The maximum levels of Cd, Cu, Pb and Zn were found in the subtidal sediments and barnacles at the oil tanker loading Single Buoy Mooring stations in Mina Al Fahal. In general, the levels of most of the contaminants analysed are at low to moderate concentrations compared to those in highly contaminated sites such as shipyards and dry docks, but continued monitoring is recommended especially during any dredging campaigns. Copyright © 2017 Elsevier Ltd. All rights reserved.

  20. An approach to 3D modeling of marine platforms in operation; Un enfoque al modelado 3D de plataformas marinas en operacion

    Energy Technology Data Exchange (ETDEWEB)

    Gomez Camargo, Octavio [Instituto de Investigaciones Electricas, Cuernavaca, Morelos (Mexico)

    2007-07-01

    During the useful life of the marine platforms it is of great importance to have an intelligent three-dimensional model, that faithfully represents the facilities, in order to optimize the operative and maintenance procedures as well as to have a real information of the condition of the equipment and of each one of the components to facilitate the decision making, to make a risk analysis and dangerous operation analyses (HAZOP) and to make designs of improvements, modernization, substitutions and extensions. At the moment, the marine platforms are being designed with CAD tools, reason why before its installation they have a 3D model, which is updated as the design advances, until arriving at the approved version for construction. Later, the digital model of the marine platform serves to guide in the construction and follow up the advance of the same. Similarly, the model 3D is due to update with respect to the changes that arise during the construction and installation, until the commissioning of the platform, nevertheless, many of these were constructed when these tools were not yet available, reason why it becomes necessary to construct the digital model according to the present condition they exhibit. In order to develop the digital model of the marine platforms in operation, the activities are grouped in three large stages: specification of scope, retrieval of the information and modeling. [Spanish] Durante la vida util de las plataformas marinas es de gran importancia contar con un modelo tridimensional inteligente, que represente fielmente las instalaciones, con la finalidad de optimizar los procedimientos operativos y de mantenimiento asi como de disponer de informacion real del estado de los equipos y de cada uno de los componentes para facilitar la toma de decisiones, para hacer analisis de riesgos y analisis de operaciones peligrosas (HAZOP) y para hacer disenos de mejoras, modernizaciones, sustituciones y ampliaciones. Actualmente, las plataformas

  1. Remote Sensing of Shallow Coastal Benthic Substrates: In situ Spectra and Mapping of Eelgrass (Zostera marina in the Gulf Islands National Park Reserve of Canada

    Directory of Open Access Journals (Sweden)

    Tara Sharma

    2011-05-01

    Full Text Available Eelgrass (Zostera marina is a keystone component of inter- and sub-tidal ecosystems. However, anthropogenic pressures have caused its populations to decline worldwide. Delineation and continuous monitoring of eelgrass distribution is an integral part of understanding these pressures and providing effective coastal ecosystem management. A proposed tool for such spatial monitoring is remote imagery, which can cost- and time-effectively cover large and inaccessible areas frequently. However, to effectively apply this technology, an understanding is required of the spectral behavior of eelgrass and its associated substrates. In this study, in situ hyperspectral measurements were used to define key spectral variables that provide the greatest spectral separation between Z. marina and associated submerged substrates. For eelgrass classification of an in situ above water reflectance dataset, the selected variables were: slope 500–530 nm, first derivatives (R’ at 566 nm, 580 nm, and 602 nm, yielding 98% overall accuracy. When the in situ reflectance dataset was water-corrected, the selected variables were: 566:600 and 566:710, yielding 97% overall accuracy. The depth constraint for eelgrass identification with the field spectrometer was 5.0 to 6.0 m on average, with a range of 3.0 to 15.0 m depending on the characteristics of the water column. A case study involving benthic classification of hyperspectral airborne imagery showed the major advantage of the variable selection was meeting the sample size requirements of the more statistically complex Maximum Likelihood classifier. Results of this classifier yielded eelgrass classification accuracy of over 85%. The depth limit of eelgrass spectral detection for the AISA sensor was 5.5 m.

  2. Valoración económica del buceo como estrategia de uso sostenible de la biodiversidad marina, Archipielago de San Andres y Providencia, Caribe Colombiano.

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    James Cruz. Johannie L.

    2011-11-01

    Full Text Available El buceo es un uso alternativo y no consuntivo de la biodiversidad marina que, debidamente practicado, tiene bajo impacto ambiental, estimula la conservación de los arrecifes y es una opción económica para habitantes de áreas arrecifales o con potencial para el buceo. El archipiélago de San Andrés, Providencia y Santa Catalina, declarado por UNESCO Reserva Mundial de la Biosfera Seafl ower, con 65.000 km2 de Áreas Marinas Protegidas y un extenso complejo arrecifal coralino, tiene condiciones para convertirse en un sitio de buceo de nivel mundial. Sin embargo, es poco conocido. Esta investigación analiza el potencial económico del buceo en esta región y lo compara con el de sitios muy reconocidos como Bonaire y Gran Caimán. Mediante análisis de la disponibilidad a pagar de buzos visitantes, se intenta valorar el buceo y se caracteriza el tipo de buzos que vienen al archipiélago. Se encontró disposición a pagar cifras superiores a las que se paga actualmente en otras partes del Caribe. Con un potencial estimado en $241 millones de dólares de ingresos anuales, el buceo es una interesante alternativa de desarrollo económico sostenible para estas islas que, a pesar de recibir 360.000 turistas al año, aún presentan un 66.9% de pobreza y altos niveles de desempleo.

  3. Study of protective effect of Avicennia marina hydroethanolic leaf extract on testes tissue and spermatogenesis in male rat induced with carbon tetrachloride

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    Z soleimani

    2015-11-01

    Full Text Available Aim: The toxic chemical compounds are widelyused in the world. Carbon tetrachloride which is used in hygiene industries caused tissue disorders. Medicinal plants have protective effects in body tissues. In this study the protective effect of Avicennia marina leaf extract (MLE on spermatogenesis in male rat were induced with ccl4 investigating. Method and materials: The 42 male rats with 220-250 gr body weight were divided randomly in 6 groups(n=7: control (taking normal saline,0.5ml/day, i,p , sham(taking olive oil, 0.5ml/day, i,p single dose,group induced by ccl4(carbon tetrachloride 1:1 with olive oil,0.5ml single dose, i,p, treated groups: (1,2 and3 by carbon tetrachloride 1:1 with olive oil,0.5ml single dose and 200mg/Kg, 400mg/Kg and 800mg/kg MLE /day for 96 hrs, i,p. After the examination the blood samples were collected from heart directly and testosterone , FSH, LH , sperm count, sperm motility and GSI were analyzed and the microscopic studies of testes tissue were done. All data were expressed as mean±SEM. and statistical significance differences were accepted at P<0.05. Results: Our results showed that the carbon tetrachloride has necrotic effect in testes. The number of sperm and motility were increased and microscopic study of testes tissue showed the necrosis and inflammation with decrease in spermatogonia and spermatocytes comparedwith ccl4 induced only group significantly (P<0.001 and treated groups were no changed. Conclusion: the Avicennia marina hydroethanolic extract has antioxidant and flavonoids compounds which can protect the testes tissues from toxic chemical agents.

  4. Caracterización cualitativa del zooplancton del Área de Conservación Marina Isla del Coco (ACMIC), Océano Pacífico de Costa Rica

    OpenAIRE

    Morales-Ramírez, Álvaro

    2016-01-01

    Qualitative characterization of the zooplankton of the Cocos Island Marine Conservation Area (Área de Conservación Marina Isla del Coco, ACMIC), Pacific Ocean of Costa Rica. During November 2001, zooplankton samples were obtained from sites around Isla del Coco, a protected oceanic island off the Pacific coast of Costa Rica. Vertical (0-50 m) and horizontal hauls were performed. This first qualitative analysis of the zooplankton shows a very diverse community, with copepods being dominant (68...

  5. Modelling reveals endogenous osmotic adaptation of storage tissue water potential as an important driver determining different stem diameter variation patterns in the mangrove species Avicennia marina and Rhizophora stylosa.

    Science.gov (United States)

    Vandegehuchte, Maurits W; Guyot, Adrien; Hubeau, Michiel; De Swaef, Tom; Lockington, David A; Steppe, Kathy

    2014-09-01

    Stem diameter variations are mainly determined by the radial water transport between xylem and storage tissues. This radial transport results from the water potential difference between these tissues, which is influenced by both hydraulic and carbon related processes. Measurements have shown that when subjected to the same environmental conditions, the co-occurring mangrove species Avicennia marina and Rhizophora stylosa unexpectedly show a totally different pattern in daily stem diameter variation. Using in situ measurements of stem diameter variation, stem water potential and sap flow, a mechanistic flow and storage model based on the cohesion-tension theory was applied to assess the differences in osmotic storage water potential between Avicennia marina and Rhizophora stylosa. Both species, subjected to the same environmental conditions, showed a resembling daily pattern in simulated osmotic storage water potential. However, the osmotic storage water potential of R. stylosa started to decrease slightly after that of A. marina in the morning and increased again slightly later in the evening. This small shift in osmotic storage water potential likely underlaid the marked differences in daily stem diameter variation pattern between the two species. The results show that in addition to environmental dynamics, endogenous changes in the osmotic storage water potential must be taken into account in order to accurately predict stem diameter variations, and hence growth.

  6. Human-induced hydrological changes and sinkholes in the gypsum karst of Lesina Marina area (Foggia Province, Italy)

    Science.gov (United States)

    Fidelibus, M. D.; Gutierrez, F.; Spilotro, G.

    2009-04-01

    the piezometric level and the associated bidirectional changes of the groundwater flow in the vicinity of the canal play a relevant role in the internal erosion processes. The low cohesion of the sandy cover determines to a great extent the high speed at which suffosion processes and the generation-enlargement of sinkholes are taking place. Subsidence activity has affected the canal since its construction. To our knowledge, the first account of a sinkhole occurrence in the adjacent area corresponds to an official report dating back to 1990. The great majority of the sinkholes are located within the canal and on two relatively narrow bands situated on its flanks. The sinkholes tend to form clusters and alignments with a prevalent N145E orientation. The Lesina Marina residential area, whose construction in the western side of the canal started around 1980, is currently suffering from subsidence damage, including the occurrence of collapse sinkholes in streets, destruction of pathways and cracking of walls. Boreholes and geophysical surveys performed in the area reveal the presence of abundant cavities up to 9 m in height, cave fills and collapse breccias in the strongly karstified bedrock. Most of the depressions can be classified as cover suffosion and collapse sinkholes generated by the downward migration of the loose sandy cover through voids in the bedrock. The lack of basal support caused by piping may lead to the gradual settlement of the cover and/or its collapse through the development of failure planes. These sinkholes are typically less than 1 m across and 2-3 m deep at the initial stages. However, they typically grow very rapidly by mass wasting processes acting on their edges until they reach the repose angle of the detrital mantle. Consequently, clusters of small sinkholes tend to evolve into a smaller number of large depressions up to 20 m meters across resulting from the coalescence of several dolines. Some sinkholes are related to the breakdown of

  7. Evaluación de un filtro biológico como unidad de post-tratamiento de aguas residuales utilizando conchas marinas como material de soporte

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    Andres Galindo

    2016-12-01

    Full Text Available En esta investigación se evaluó la eficiencia de un filtro biológico (FB a escala piloto, utilizando conchas marinas como material de soporte (MS para el post-tratamiento de aguas residuales municipales (ARM que provenían de un tratamiento previo anaerobio-aerobio. El FB se evaluó con diferentes cargas orgánicas, siendo los tiempos de retención hidráulicos (TRH estudiados: 12,85; 10,71; 8,57 y 6,43h. Los parámetros monitoreados fueron: pH, alcalinidad carbónica y total, temperatura, demanda química de oxígeno (DQO, demanda bioquímica de oxígeno (DBO, ácidos grasos volátiles (AGV, nitrógeno total Kjeldahl (NTK, fósforo total, sólidos suspendidos totales (SST, volátiles (SSV y fijos (SSF. Las mejores eficiencias de remoción de materia orgánica se obtuvieron a TRH de 10,71h, registrando remociones de DQOT, DQOS y DBO5,20 en el orden de 51,5; 48,6 y 39,2%. Sin embargo, la mejor eficiencia en la remoción de sólidos suspendidos se observó en el TRH de 6,43h (SST, 29%, SSV, 23,8% y SSF, 50%. A pesar de recibir tratamiento previo, el número de coliformes totales y fecales afluentes al FB fueron altos, presentando órdenes hasta de 107NMP/100ml, la mayores eficiencias en la remoción de Coliformes totales y fecales fueron de 97,24 y 94,63%, respectivamente, registradas en el TRH de 12,85h. Se evidencia que las conchas marinas (Arca zebra son MS sostenibles y con ellas se logran reducciones satisfactorias de materia orgánica en el tratamiento de ARM.

  8. Depredación de aves marinas nocturnas en islotes de isla Guadalupe, México Predation of nocturnal seabirds on islets of Isla Guadalupe, Mexico

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    Eric Mellink

    2010-08-01

    Full Text Available A principios de junio de 2000, se realizó una prospección biológica de 3 islotes en el extremo sur de isla Guadalupe: islote de Afuera (o islote Zapato, islote de Adentro (o islote Toro e islote Negro, para documentar aves marinas anidantes. Se detectaron despojos de más de 100 paíños de Leach (Oceanodroma leucorhoa, 31 mérgulos de Xantus (Synthliboramphus hypoleuca y un alcuela de Cassin (Ptychoramphus aleuticus depredados. Las evidencias indican como el depredador de los paíños al tecolote llanero (Athene cunicularia, pero debido a su tamaño posiblemente no lo haya sido de los mérgulos, ni de la alcuela. Las características de los restos de los individuos depredados indican que eran de la población de paíños que anidó el invierno inmediato anterior. La información no es suficiente para determinar si dicha depredación representa un problema de conservación de aves marinas pequeñas en los islotes de la isla Guadalupe, pero sí sugiere que se debe estudiar más a fondo.In early June 2000 we surveyed 3 islets at the southern end of Isla Guadalupe, Mexico: Islote de Afuera (or Islote Zapato, Islote de Adentro (or islote Toro, and Islote Negro, to document nesting seabirds. We found vestiges of over 100 Leach's Storm-petrels (Oceanodroma leucorhoa, 31 Xantus Murrelets (Synthliboramphus hypoleuca, and 1 Cassin's Auklet (Ptychoramphus aleuticus that had been depredated. The evidences point to Burrowing Owls (Athene cunicularia as the predator of the storm-petrels, but, because of its size, probably other birds were involved in murrelet and auklet predation. Based on the characteristics of the remains, the depredated Leach Storm-petrels belonged to the population that had nested during the previous winter. The information is not sufficient to determine whether such predation represents a conservation problem for small seabirds on Isla Guadalupe, but suggest that the issue should be studied further.

  9. Appraisal of the groundwater conceptual model of the gypsum coastal karst of Lesina Marina (Puglia, Southern Italy) aiming at density-dependent modeling.

    Science.gov (United States)

    Campana, C.; Fidelibus, M. D.

    2012-04-01

    Inhabited coastal areas are site of many risks, because in terms of geomorphology, hydraulics, hydrogeology and mass transport, they are at the lowest level of potential energy. Moreover, considering that at land-sea interface brackish/salt waters enhance in general the effects of water-rock interactions, the number of possible risks increases. This is the case of the gypsum coastal karst of Lesina Marina (Puglia, Southern Italy), where, starting from 1993, a number of sinkholes developed mostly along an artificial canal, excavated in 1927 to connect a brackish lagoon with the sea. The excavation diverted groundwater flow, with increase of hydraulic gradients and filtration velocity, amplitude of the groundwater level oscillations and, locally, tidal efficiency, causing continuous "flooding and draining" cycles, largely controlled by tides. These factors, on the whole, favored internal erosion and flushing of cave deposits, and amplified, due to increased hydraulic connectivity between fresh and brackish/salt water, gypsum dissolution. After many years it is still not likely to assess the relative role played by internal erosion and dissolutional enlargement of cavities in the sinkhole development. With the final aim of defining the subsidence risk in the Lesina Marina area, recently the Puglia River Basin Authority realized new bore-holes, geophysical logs and a continuous piezometric monitoring. To the aims of the reconstruction of the aquifer conceptual model and of the identification of key factors for a reliable numerical density-dependent modeling (of flow, solute-transport and reactions), in the period from September to December 2011 we realized Electrical Conductivity and Temperature logs, samplings (at different depths) and chemical analyses along two transects perpendicular to the canal. Apart from the recognition of the main water-rock interaction processes (as gypsum solution and inverse Na/Ca ion-exchange), and the reconstruction of the trends of

  10. Análisis multicriterio del impacto potencial del turismo en la anidación de las tortugas marinas en Chalacatepec, Jalisco

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    Y. Flores-Monter

    2015-01-01

    Full Text Available Introducción: El litoral mexicano presenta hábitats aptos para la reproducción, refugio y crianza de tortugas marinas. Sin embargo, en la costa Chalacatepec, Jalisco, esta aptitud puede estar amenazada por el Proyecto Desarrollo Vistas (PDV, un complejo turístico mixto: hotelero, residencial, comercial y deportivo. El objetivo de la presente investigación, es evaluar el impacto potencial del turismo sobre la anidación de tortugas en dos etapas: a Preparación y construcción y b Operación y mantenimiento, como una herramienta de ayuda a la toma de decisiones en la conservación de las especies Lepidochelys olivácea (tortuga golfina, Chelonia agassizii (tortuga prieta y Dermochelys coriácea (tortuga laúd. Método: Se calculó la densidad de anidación (nidos por kilómetro y se utilizó el método de evaluación multicriterio (EMC discreto Proceso Analítico Jerárquico (PAJ, para estimar el impacto de 8 amenazas turísticas: desmonte y despalme, excavaciones, rellenos, contaminación sonora, residuos sólidos, presencia de personal de obra y construcciones provisionales, iluminación artificial del frente de playa y concentración de visitantes. Se construyó una matriz de comparación, se asignó un peso a cada amenaza, y mediante el uso de un SIG se generaron los mapas de impacto potencial del turismo. Resultados: Las amenazas con mayor peso o ponderación (eigenvector total fueron los rellenos (0.36482 y la concentración de visitantes (0.38194. La construcción y operación turística puede afectar el 59.8% de las anidaciones e impactar principalmente en la costa sur el km 1 (1 142 registros de nidos colectados y, en la costa norte el km 1 (329 nidos, km 3 (311 nidos, km 4 (268 nidos y km 5 (151 nidos. Discusión o Conclusión: La playa de anidación en Chalacatepec es de gran importancia para la protección y conservación de las tortugas marinas. La presente EMC fue una herramienta adecuada para estimar las consecuencias del

  11. Genetic damage in Rhinella marina populations in habitats affected by agriculture in the middle region of the Sinú River, Colombia.

    Science.gov (United States)

    Cruz-Esquivel, Ángel; Viloria-Rivas, Jose; Marrugo-Negrete, Jose

    2017-10-03

    Contamination with pesticide residues affects the environmental health of agroecosystems, especially the amphibian fauna that lives in these environments. The objective of the present study was to determine pesticides concentrations in sediments of agroecosystems and to evaluate genetic damage in Rhinella marina populations living in these zones. A total of 91 individuals were collected, 51 in the group exposed in different areas of the middle region of the Sinú River (Irrigation District of Mocari 16, Irrigation District of Aguas Negras 21, Irrigation District of Cerete 14) and 40 in a control group; at the same time, 36 subsamples of sediments were taken at each sampled station to determine pesticides organochlorine by means of chromatography coupled with ISQ Thermo Scientific mass spectrometer. The micronucleus test was applied in erythrocytes of the individuals collected. Results showed the presence of persistent organochlorine pesticides (POPs) in the sediment samples (p,p'-DDT, p,p'-DDE, and p,p'-DDD) of agricultural soils. Two individuals were registered with abnormalities in their limbs at the Mocari station, representing 12.5% of the morphological malformations to this sector. Micronucleus analysis revealed statistically significant genetic damage in exposed individuals (Mocari 9.87 ± 5.1, Cerete 7.7 ± 1.7, Aguas Negras 5.6 ± 3.6) with respect to the control group (2.4 ± 1.9) (p < 0.05). Spearman correlation analysis revealed a positive association between genetic damage and POP concentrations (p < 0.05). In addition, cellular alterations such as nuclear buds, and pyknosis (cell death), were statistically significant in the exposed group compared to the control group (p < 0.05). This study suggests that there is evidence for morphological and genotoxic effects in R. marina populations inhabiting areas influenced by agriculture, possibly associated with the presence of p,p'-DDT, p,p'-DDD, and p,p'-DDE.

  12. Algas marinas bentónicas de la costa noroccidental de Guerrero, México Benthic marine algae of the west coast of Guerrero, Mexico

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    Luz Elena Mateo-Cid

    2012-12-01

    Full Text Available Se presentan los resultados de un estudio sobre algas marinas bentónicas en 7 localidades de la costa noroccidental de Guerrero, México. Se determinó la presencia de 163 especies de algas marinas. Se identificaron 17 especies de Cyanobacteria, 93 Rhodophyta, 28 Chlorophyta y 25 de Heterokontophyta. Se citan 54 registros nuevos para el litoral de Guerrero, 2 también nuevos, Myrionema strangulans Greville y Acrochaete ramosa (N.L.Gardner O'Kelly para la costa del Pacífico. Cada especie se acompaña de datos sobre su distribución en el área de estudio, su estado reproductivo, nivel de marea, hábitat, observaciones, epifitismo y número de herbario o de recolección. Se comparó la riqueza específica entre la estación climática de lluvias y la de secas. La división Rhodophyta dominó en términos de diversidad en relación con las 3 divisiones restantes. La ficoflora de la costa noroccidental de Guerrero es de afinidad tropical y más diversa en la época de secas.We present results on the study on benthic marine algae in 7 localities from the west coast of Guerrero, Mexico. We report 163 species: 17 Cyanobacteria, 93 Rhodophyta, 28 Chlorophyta and 25 Heterokontophyta. Fifty four are new records for Guerrero; while Myrionema strangulans Greville and Acrochaete ramosa (N.L.Gardner O'Kelly are new to the Pacific coast of Mexico. Each species includes data on its distribution, reproductive stages, tidal level, facies, epiphytism and herbarium's number. Species diversity was compared for 2 different climatic seasons. The Rhodophyta are dominant in terms of diversity in relation to the other groups. The algal flora of the northwest coast of Guerrero is tropical and the greatest diversity was found during dry seasons.

  13. ‘A Timeless Grammar of Iconoclasm?’: Kristine Kolrud and Marina Prusac (eds, Iconoclasm From Antiquity to Modernity, Farnham: Ashgate, 2014

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    Lauren Dudley

    2014-12-01

    Full Text Available This review examines Kristine Kolrud and Marina Prusac’s edited volume, Iconoclasm From Antiquity to Modernity. The collection of essays covers a broad historical and methodological scope and explores the motives and discourses related to iconoclastic acts, including written sources about iconoclasts and iconophiles. It also considers terminology associated with iconoclastic acts and, through its consideration of modern case studies, proffers various categories of intention. The examples discussed by each contributor raise questions relating to the methods of destruction as well as written accounts about acts of iconoclasm but, overall, the theoretical structure of the volume might be regarded as lacking the critical acumen evident by Bruno Latour and Peter Weibel in their larger scale Iconoclash catalogue. Nevertheless, this volume’s contributors explore themes such as memory and power struggles, while also addressing the reliability of material and written sources. This review provides an analysis of each chapter and how they relate to the overall volume and, indeed, existing scholarship on iconoclasm.

  14. Development and application of long-term sublethal whole sediment tests with Arenicola marina and Corophium volutator using Ivermectin as the test compound.

    Science.gov (United States)

    Allen, Yvonne T; Thain, John E; Haworth, Sarah; Barry, Jon

    2007-03-01

    Short-term whole sediment tests using the amphipod Corophium volutator and the polychaete Arenicola marina are now routinely used in Europe to assess the acute toxicity of marine sediments. However, there is still a need to develop longer-term assays which measure effects on sublethal endpoints that are more relevant to predicting impacts at the population level. The effect of increasing exposure times and measuring additional endpoints such as growth, on the sensitivity of these assays was investigated. The test compound used was the chemotherapeutant Ivermectin (IVM), used in aquaculture to treat sea lice infestations. IVM was found to be acutely toxic to both test organisms. Extending the lugworm test to 100 days increased sensitivity of survival by a factor of three; a significant reduction in casting rate was observed at concentrations an order of magnitude lower. This assay shows potential for detecting the sublethal effects of low concentrations of sediment contaminants. Increasing the exposure time did not seem to affect the sensitivity of the amphipod, but further method development is required.

  15. Development and application of long-term sublethal whole sediment tests with Arenicola marina and Corophium volutator using Ivermectin as the test compound

    Energy Technology Data Exchange (ETDEWEB)

    Allen, Yvonne T. [Centre for Environment, Fisheries and Aquaculture Science, Burnham Laboratory, Remembrance Avenue, Burnham-on-Crouch, Essex, CM0 8HA (United Kingdom)]. E-mail: yvonne.allen@cefas.co.uk; Thain, John E. [Centre for Environment, Fisheries and Aquaculture Science, Burnham Laboratory, Remembrance Avenue, Burnham-on-Crouch, Essex, CM0 8HA (United Kingdom); Haworth, Sarah [Centre for Environment, Fisheries and Aquaculture Science, Burnham Laboratory, Remembrance Avenue, Burnham-on-Crouch, Essex, CM0 8HA (United Kingdom); Barry, Jon [Centre for Environment, Fisheries and Aquaculture Science, Burnham Laboratory, Remembrance Avenue, Burnham-on-Crouch, Essex, CM0 8HA (United Kingdom)

    2007-03-15

    Short-term whole sediment tests using the amphipod Corophium volutator and the polychaete Arenicola marina are now routinely used in Europe to assess the acute toxicity of marine sediments. However, there is still a need to develop longer-term assays which measure effects on sublethal endpoints that are more relevant to predicting impacts at the population level. The effect of increasing exposure times and measuring additional endpoints such as growth, on the sensitivity of these assays was investigated. The test compound used was the chemotherapeutant Ivermectin (IVM), used in aquaculture to treat sea lice infestations. IVM was found to be acutely toxic to both test organisms. Extending the lugworm test to 100 days increased sensitivity of survival by a factor of three; a significant reduction in casting rate was observed at concentrations an order of magnitude lower. This assay shows potential for detecting the sublethal effects of low concentrations of sediment contaminants. Increasing the exposure time did not seem to affect the sensitivity of the amphipod, but further method development is required. - Long-term sublethal sediment tests are more sensitive than standard acute tests when assessing the toxic effects of Ivermectin.

  16. Wenzhouxiangella marina gen. nov, sp. nov, a marine bacterium from the culture broth of Picochlorum sp. 122, and proposal of Wenzhouxiangellaceae fam. nov. in the order Chromatiales.

    Science.gov (United States)

    Wang, Guanghua; Tang, Mingxing; Li, Tao; Dai, Shikun; Wu, Huanlian; Chen, Chenghao; He, Hui; Fan, Jiewei; Xiang, Wenzhou; Li, Xiang

    2015-06-01

    A Gram-stain negative, non-motile, non-phototrophic, non-alkaliphilic, obligately aerobic, chemoheterotrophic, and rod-shaped bacterium, designated strain Ma-11(T), was isolated from the culture broth of a marine microalga, Picochloruma sp. 122. Phylogenetic analyses showed that strain Ma-11(T) has less than 91 % similarity to its closest relative, Thioalkalivibrio sulfidiphilus HL-EbGR7(T), represents a distinct phylogenetic lineage in the order Chromatiales, and could not be assigned to any defined families in this order. Chemotaxonomic, genetic and physiological characteristics, including major fatty acids, genomic G+C content, lack of motility, aerophilicity and chemoheterotrophicity, could readily distinguish strain Ma-11(T) from any established members of the order Chromatiales. Based on the 16S rRNA gene sequence analysis and its signature nucleotide pattern, a new family Wenzhouxiangellaceae fam. nov. comprising the genus Wenzhouxiangella gen. nov. and species Wenzhouxiangella marina sp. nov. is proposed. The type strain is Ma-11(T) (=CGMCC 1.14936(T) = KCTC 42284(T) = MCCC 1K00261(T)).

  17. Vitamin A values of wild-caught Cuban tree frogs (Osteopilus septentrionalis) and marine toads (Rhinella marina) in whole body, liver, and serum.

    Science.gov (United States)

    Sullivan, Kathleen E; Fleming, Greg; Terrell, Scott; Smith, Dustin; Ridgley, Frank; Valdes, Eduardo V

    2014-12-01

    Recent issues surrounding captive amphibians are often nutritionally related problems, such as hypovitaminosis A. Although supplementation of frogs with vitamin A is a topic of investigation, the underlying issue is understanding vitamin A metabolism in amphibian species. To develop a range of "normal" vitamin A concentrations for captive amphibians, baseline vitamin A concentrations must be established in wild amphibian species. In this study, two species, Cuban tree frogs (Osteopilus septentrionalis; n = 59) and marine toads (Rhinella marina; n = 20) were collected from the wild as part of an invasive species control program at Zoo Miami, Miami, Florida. Serum, liver, and whole body samples were analyzed for vitamin A content. The Cuban tree frogs showed higher concentrations on average of vitamin A in serum (82.8 ppb), liver (248.3 IU/g), and whole body (5474.7 IU/kg) samples compared with marine toads (60.1 ppb; 105.3 IU/g; 940.7 IU/kg, respectively), but differences were not significant (P = 0.22). What can be considered "normal" values of vitamin A concentrations across different amphibian species requires further investigation. Although all amphibians collected in this study appeared healthy, a larger sample size of animals, with known health histories and diets, may provide stronger evidence of normal expectations.

  18. Lista de esponjas marinas asociadas al arrecife Tuxpan, Veracruz, México Checklist of marine sponges from Tuxpan Reef, Veracruz, Mexico

    Directory of Open Access Journals (Sweden)

    Carlos González-Gándara

    2009-04-01

    Full Text Available Se presenta la lista de esponjas marinas (Porifera: Demospongiae del arrecife Tuxpan, Veracruz, México, colectadas en 2004, 2005 y 2006 mediante buceo libre y con equipo autónomo SCUBA. Los resultados muestran la presencia de 18 especies pertenecientes a 13 géneros y 13 familias, 17 de estas especies son nuevos registros para los arrecifes coralinos del norte de Veracruz y una (Aplysina cauliformis Carter, 1882 para el estado. La información puede auxiliar para definir las estrategias de manejo, monitoreo y protección de estas formaciones arrecifales que recientemente han sido propuestas como área de protección de flora y fauna.A checklist of marine sponge species (Porifera: Demospongiae from Tuxpan reef, Veracruz, Mexico, collected during 2004, 2005 and 2006 by free and SCUBA diving equipment, is presented. The results show the presence of 18 species belonging to 13 genera and 13 families. 17 speices represent new records for the northern coral reefs of Veracruz, and the 18th species (Aplysina cauliformis Carter, 1882 is a new record for the state. This information may help to define appropriate management, monitoring and protection strategies for the coral reefs of the north of Veracruz, which have been proposed as a natural preserve area recently.

  19. Avicequinone C Isolated from Avicennia marina Exhibits 5α-Reductase-Type 1 Inhibitory Activity Using an Androgenic Alopecia Relevant Cell-Based Assay System

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    Ruchy Jain

    2014-05-01

    Full Text Available Avicennia marina (AM exhibits various biological activities and has been traditionally used in Egypt to cure skin diseases. In this study, the methanolic heartwood extract of AM was evaluated for inhibitory activity against 5α-reductase (5α-R [E.C.1.3.99.5], the enzyme responsible for the over-production of 5α-dihydrotestosterone (5α-DHT causing androgenic alopecia (AGA. An AGA-relevant cell-based assay was developed using human hair dermal papilla cells (HHDPCs, the main regulator of hair growth and the only cells within the hair follicle that are the direct site of 5α-DHT action, combined with a non-radioactive thin layer chromatography (TLC detection technique. The results revealed that AM is a potent 5α-R type 1 (5α-R1 inhibitor, reducing the 5α-DHT production by 52% at the final concentration of 10 µg/mL. Activity-guided fractionation has led to the identification of avicequinone C, a furanonaphthaquinone, as a 5α-R1 inhibitor with an IC50 of 9.94 ± 0.33 µg/mL or 38.8 ± 1.29 µM. This paper is the first to report anti-androgenic activity through 5α-R1 inhibition of AM and avicequinone C.

  20. Kinetic studies of peroxiredoxin 6 from Arenicola marina: rapid oxidation by hydrogen peroxide and peroxynitrite but lack of reduction by hydrogen sulfide.

    Science.gov (United States)

    Loumaye, Eléonore; Ferrer-Sueta, Gerardo; Alvarez, Beatriz; Rees, Jean-François; Clippe, André; Knoops, Bernard; Radi, Rafael; Trujillo, Madia

    2011-10-01

    Arenicola marina lives in marine environments where hydrogen peroxide concentrations reach micromolar levels. The annelid also forms reactive species through metabolic pathways. Its antioxidant systems include a cytosolic peroxiredoxin, peroxiredoxin 6 (AmPrx6 or AmPRDX6) that shows high homology to the mammalian 1-Cys peroxiredoxin. Previous work confirmed the peroxidase activity of AmPrx6 in the presence of dithiotreitol. Herein, we performed an in vitro kinetic characterization of the recombinant enzyme. AmPrx6 reduced hydrogen peroxide and peroxynitrite with rate constants of 1.1×10(7) and 2×10(6)M(-1)s(-1), respectively, at pH 7.4 and 25°C. Reduction of tert-butyl hydroperoxide was slower. The pK(a) of the peroxidatic thiol of AmPrx6 was determined as 5.1±0.2, indicating that it exists as thiolate, the reactive species, at physiological pH. The reductive part of the catalytic cycle was also explored. Hydrogen sulfide, present in millimolar concentrations in marine sediments where the annelid lives and that is able to reduce the mammalian 1-Cys peroxiredoxin, did not support AmPrx6 peroxidase activity. The enzyme was not reduced by other potential physiological reductants tested. Our data indicate that in this annelid, Prx6 could contribute to peroxide detoxification in the presence of a so far unidentified reducing counterpart. Copyright © 2011 Elsevier Inc. All rights reserved.

  1. Pollutant tracking for 3 Western North Atlantic sea grasses by remote sensing: Preliminary diminishing white light responses of Thalassia testudinum, Halodule wrightii, and Zostera marina.

    Science.gov (United States)

    Thorhaug, Anitra; Berlyn, Graeme P; Poulos, Helen M; Goodale, Uromi M

    2015-08-15

    Sea grasses are foundation species for estuarine ecosystems. The available light for sea grasses diminishes rapidly during pollutant spills, effluent releases, disturbances such as intense riverine input, and tidal changes. We studied how sea grasses' remote-sensing signatures and light-capturing ability respond to short term light alterations. In vivo responses were measured over the entire visible-light spectra to diminishing white-light on whole-living-plants' spectral reflectance, including 6h of full oceanic-light fluences from 10% to 100%. We analyzed differences by various reflectance indices. We compared the sea grasses species responses of tropical vs. temperate and intertidals (Halodule wrightii, and Zostera marina) vs. subtidal (Thalassia testudinum). Reflectance diminished with decreasing light intensity that coincided with greater accessory pigment stimulation (anthocyanin, carotenoids, xanthins). Chlorophyll a and Chlorophyll b differed significantly among species (Thalassia vs. Halodule). Photosynthetic efficiency diminished at high light intensities. The NDVI index was inadequate to perceive these differences. Our results demonstrate the leaf-level utility of data to remote sensing for mapping sea grass and sea grass stress. Copyright © 2015 Elsevier Ltd. All rights reserved.

  2. Multiple markers pyrosequencing reveals highly diverse and host-specific fungal communities on the mangrove trees Avicennia marina and Rhizophora stylosa.

    Science.gov (United States)

    Arfi, Yonathan; Buée, Marc; Marchand, Cyril; Levasseur, Anthony; Record, Eric

    2012-02-01

    Fungi are important actors in ecological processes and trophic webs in mangroves. Although saprophytic fungi occurring in the intertidal part of mangrove have been well studied, little is known about the diversity and structure of the fungal communities in this ecosystem or about the importance of functional groups like pathogens and mutualists. Using tag-encoded 454 pyrosequencing of the ITS1, ITS2, nu-ssu-V5 and nu-ssu-V7 regions, we studied and compared the fungal communities found on the marine and aerial parts of Avicennia marina and Rhizophora stylosa trees in a mangrove in New Caledonia. A total of 209,544 reads were analysed, corresponding to several thousand molecular operational taxonomic units (OTU). There is a marked zonation in the species distribution, with most of the OTU being found specifically in one of the microhabitat studied. Ascomycetes are the dominant phylum (82%), Basidiomycetes are very rare (3%), and 15% of the sequences correspond to unknown taxa. Our results indicate that host specificity is a key factor in the distribution of the highly diverse fungal communities, in both the aerial and intertidal parts of the trees. This study also validates the usefulness of multiple markers in tag-encoded pyrosequencing to consolidate and refine the assessment of the taxonomic diversity. © 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.

  3. Mixed population genomics support for the central marginal hypothesis across the invasive range of the cane toad (Rhinella marina) in Australia.

    Science.gov (United States)

    Trumbo, Daryl R; Epstein, Brendan; Hohenlohe, Paul A; Alford, Ross A; Schwarzkopf, Lin; Storfer, Andrew

    2016-09-01

    Understanding factors that cause species' geographic range limits is a major focus in ecology and evolution. The central marginal hypothesis (CMH) predicts that species cannot adapt to conditions beyond current geographic range edges because genetic diversity decreases from core to edge due to smaller, more isolated edge populations. We employed a population genomics framework using 24 235-33 112 SNP loci to test major predictions of the CMH in the ongoing invasion of the cane toad (Rhinella marina) in Australia. Cane toad tissue samples were collected along broad-scale, core-to-edge transects across their invasive range. Geographic and ecological core areas were identified using GIS and habitat suitability indices from ecological niche modelling. Bayesian clustering analyses revealed three genetic clusters, in the northwest invasion-front region, northeast precipitation-limited region and southeast cold temperature-limited region. Core-to-edge patterns of genetic diversity and differentiation were consistent with the CMH in the southeast, but were not supported in the northeast and showed mixed support in the northwest. Results suggest cold temperatures are a likely contributor to southeastern range limits, consistent with CMH predictions. In the northeast and northwest, ecological processes consisting of a steep physiological barrier and ongoing invasion dynamics, respectively, are more likely explanations for population genomic patterns than the CMH. © 2016 John Wiley & Sons Ltd.

  4. ANALISA ELECTRODE CONSUMABLE TYPE OK AUTROD 12.10 DENGAN PENGELASAN SUBMERGED ARC WELDING (SAW PADA BLOCK KAPAL DCV 18500 DWT DI PT. JASA MARINA INDAH SEMARANG

    Directory of Open Access Journals (Sweden)

    Kiryanto Kiryanto

    2012-02-01

    Full Text Available In this of globalization is growing very rapidly advancing technology. As well as in shipyard industry is toshipbuilding process. Submerged Arch Welding (SAW is as joint methode of ship construction. Purpose ofresearch know number of electrodes wich requirement on block welding the ship DCV 18500 DWT in JasaMarina Indah Shipyard Semarang.The specimen experiment used is ST 42 low carbon steel. Variation of plates tickness is 12, 13, 14, 17, 19 and24 mm. Research step is carried out by making the specimen with variation thickness respectively. Specimendimensions length x width is 500 mm x 50 mm. The number of each specimen was made of three pieces. Thendo the measurement welding length, length and weight electrodes and slag welding.The analysis result of data welding on the specimen can be known number of electrode s and fluxs inSubmerged Arch Welding (SAW. The aplication of this research is the block DB 5(p/c/s, SS5A(p/s, SS5B(p/s, UD 5C, and TB 102 (p/c/s. Weight of consumable electrodes is 2185.31 kg (73 roll and number offlux used wight is 2967.95 kg (119 bag.

  5. Trace metals in sediments and Zostera marina of San Ignacio and Ojo de Liebre lagoons in the central pacific coast of Baja California, Mexico.

    Science.gov (United States)

    Macías-Zamora, J V; Sánchez-Osorio, J L; Ríos-Mendoza, L M; Ramírez-Alvarez, N; Huerta-Díaz, M A; López-Sánchez, D

    2008-08-01

    San Ignacio and Ojo de Liebre lagoons in central Baja California, Mexico are nursery and grazing grounds for whales and turtles. Ojo de Liebre Lagoon also supports a salt mine operation. By concentrating trace metals via evaporation, this activity might harm biota. Consequently, salt mining might be incompatible with the lagoon's ecological role. Eelgrass can incorporate these elements and reroute them to other organisms. Trace metals in sediments (Cd, Co, Cu, Mn, Ni, Pb, Zn, and Fe) were measured at both lagoons. Some (Cu, Mn, Pb, and Zn) were also measured in Zostera marina patches at both lagoons. The results did not show elevated metal concentration at any lagoon, either for sediments or eelgrass. No statistically significant differences between lagoons were found. However, eelgrass at both lagoons showed larger concentration ranges than in sediments. Also, a correlation exists between sediment metal concentration and its concentration in eelgrass. Surprisingly, several sediment metal concentrations are higher than those considered as elevated for the Southern California Bight.

  6. Identification and Analysis of Red Sea Mangrove (Avicennia marina) microRNAs by High-Throughput Sequencing and Their Association with Stress Responses

    KAUST Repository

    Khraiwesh, Basel

    2013-04-08

    Although RNA silencing has been studied primarily in model plants, advances in high-throughput sequencing technologies have enabled profiling of the small RNA components of many more plant species, providing insights into the ubiquity and conservatism of some miRNA-based regulatory mechanisms. Small RNAs of 20 to 24 nucleotides (nt) are important regulators of gene transcript levels by either transcriptional or by posttranscriptional gene silencing, contributing to genome maintenance and controlling a variety of developmental and physiological processes. Here, we used deep sequencing and molecular methods to create an inventory of the small RNAs in the mangrove species, Avicennia marina. We identified 26 novel mangrove miRNAs and 193 conserved miRNAs belonging to 36 families. We determined that 2 of the novel miRNAs were produced from known miRNA precursors and 4 were likely to be species-specific by the criterion that we found no homologs in other plant species. We used qRT-PCR to analyze the expression of miRNAs and their target genes in different tissue sets and some demonstrated tissue-specific expression. Furthermore, we predicted potential targets of these putative miRNAs based on a sequence homology and experimentally validated through endonucleolytic cleavage assays. Our results suggested that expression profiles of miRNAs and their predicted targets could be useful in exploring the significance of the conservation patterns of plants, particularly in response to abiotic stress. Because of their well-developed abilities in this regard, mangroves and other extremophiles are excellent models for such exploration. © 2013 Khraiwesh et al.

  7. An Experimental Comparison of the Effects of Zostera marina and Zostera japonica on the Diurnal Variability of the Carbonate System in the Context of a Pacific Northwest Estuary

    Science.gov (United States)

    Miller, C.; Love, B. A.; Yang, S.

    2016-02-01

    Atmospheric CO2 emissions are being absorbed at an unprecedented rate by the global and coastal oceans, shifting the baseline pCO2, and inducing anthropogenic ocean acidification (OA). Recent studies have highlighted the potential benefits of near-shore vegetated habitats, such as seagrass beds as carbon sinks, potentially mitigating the effects of OA for vulnerable calcifying organisms. Seagrasses are capable of raising seawater pH and CaCO3 saturation state during times of high photosynthetic activity; however, the converse occurs during periods of dark respiration, resulting in a cyclical pattern of high and low pH and saturation state. A better understanding of diurnal seagrass induced carbonate system variability is needed to determine whether seagrass beds act as refugia from OA, and if individual species differentially affect the carbonate system. We conducted experiments to compare the effects of the native Zostera marina and non-native Zostera japonica on the carbonate system. Leaf clippings were incubated at five different light intensities (including dark) and pCO2 levels, representing the range of light and pCO2 in a given day. Induced changes in dissolved inorganic carbon (DIC) via photosynthesis and respiration were measured as well as pH and alkalinity. As irradiance and concentration of bio-available inorganic carbon are the two main drivers of photosynthetic activity, our measurements of the short-term response of photosynthesis to a spectrum of pCO2 and irradiance intensity can predict the diurnal fluctuation of pH and DIC for both species. This lab study provides a mechanistic background for building complex models based on field monitoring of carbonate chemistry in seagrass communities by comparing and integrating our results with in situ measurements. Interpretation of our findings will be placed in the context of short-term seagrass response to the spatiotemporal variability of pCO2 with respect to the progression of ocean acidification.

  8. Marine diatoms from Buenos Aires coastal waters (Argentina: II. Thalassionemataceae and Rhaphoneidaceae Diatomeas marinas de aguas costeras de la Provincia de Buenos Aires (Argentina: II. Thalassionemataceae y Rhaphoneidaceae

    Directory of Open Access Journals (Sweden)

    EUGENIA A SAR

    2007-03-01

    Full Text Available This study describes the diversity of the species belonging to the diatom families Thalassionemataceae Round and Rhaphoneidaceae Forti frequently found in the temperate marine coastal waters of Buenos Aires Province, Argentina, from October 1994 to September 2000. It comprises morphological, taxonomic and distributional analysis of species found in planktonic samples collected at San Clemente del Tuyú, Santa Teresita, La Lucila del Mar, Mar de Ajó, Nueva Atlantis, Pinamar and Villa Gesell. Raw and cleaned samples were analysed with light and scanning electron microscopy. Four taxa of the genus Thalassionema Grunow ex Mereschkowsky amend. Hallegraeff, one of the genus Lioloma Hasle, two of the genus Delphineis Andrews and one of the genus Rhaphoneis Ehrenberg, were identified. Thalassionema pseudonitzschioides (Schuette & Schrader Hasle, Thalassionema synedriforme (Greville Hasle and Lioloma pacificum (Cupp Hasle in Hasle & Syvertsen are new records for ArgentinaEste estudio describe la diversidad de especies de diatomeas pertenecientes a las familias Thalassionemataceae Round and Rhaphoneidaceae Forti encontradas frecuentemente en aguas costeras marinas de la Provincia de Buenos Aires, Argentina, entre octubre de 1994 y septiembre de 2000. En él se lleva a cabo el estudio morfológico, taxonómico y distribucional de las especies halladas en muestras planctónicas colectadas en San Clemente del Tuyú, Santa Teresita, La Lucila del Mar, Mar de Ajó, Nueva Atlantis, Pinamar y Villa Gesell. Material sin tratar y tratado fue analizado con microscopio óptico y microscopio electrónico de barrido. Cuatro taxa de Thalassionema Grunow ex Mereschkowsky amend. Hallegraeff, uno de Lioloma Hasle, dos de Delphineis Andrews y uno de Rhaphoneis Ehrenberg, fueron identificados. Thalassionema pseudonitzschioides (Schuette & Schrader Hasle, Thalassionema synedriforme (Greville Hasle y Lioloma pacificum (Cupp Hasle in Hasle & Syvertsen son reportadas por

  9. Efectividad del monitoreo de la anidación de tortugas marinas para determinar el éxito reproductivo en playas del sur de Cuba

    Directory of Open Access Journals (Sweden)

    Julia Azanza-Ricardo

    2015-07-01

    Full Text Available El monitoreo de las anidaciones es muy útil como herramienta para la conservación pero el esfuerzo de trabajo en diferentes áreas puede variar grandemente. En Cuba, se aplican cuatro enfoques diferentes: monitoreo sistemático nocturno y diurno (MSN, y monitoreo esporádico con o sin comprobación de nidos (MECC. La cantidad y exactitud de los datos tomados y la calidad de la información derivada de ellos difieren. Por esta razón, en el presente trabajo se evalúa la efectividad de cada enfoque para determinar el éxito reproductivo de tortugas marinas en Cuba. El MSN sólo se realiza en las playas de anidación de la Península de Guanahacabibes, mientras que el MECC es el más extendido en el país. La porción de la temporada de anidación de cada una de las tres especies que anidan en Cuba (Chelonia mydas, Caretta caretta y Eretmochelys imbricata que se cubre con personal de monitoreo es baja para la mayoría de las playas, y sobre todo en el caso de E. imbricata. Se detectaron diferencias entre el monitoreo sistemático y esporádico, por tanto, la capacidad de detectar rastros falsos y verdaderos depende esencialmente de la frecuencia de monitoreo. Esto afecta la capacidad para evaluar el éxito de la anidación por playas. A pesar del incremento en los esfuerzos realizados en Cuba para el seguimiento de las principales colonias de anidación, se deben identificar nuevas estrategias para garantizar la correcta toma de información, y una mayor eficiencia del programa de monitoreo para obtener la mayor información posible de cada una de las especies con un adecuado balance de costo-beneficio.

  10. Linguistic and paralinguistic marks of the emotion management and ethos-building in Ms. Marina Silva´s discourse, in the talk show Jô 11/2

    Directory of Open Access Journals (Sweden)

    Cláudio Humberto Lessa

    2015-03-01

    Full Text Available De nombreuses études montrent comment les médias ont transformé l´éloquence po­litique, par exemple, Rubim (2004 et Courtine (2006. Le hommo politicus a adopté une rhétorique marquée par un style dialogué et familial aussi. Aujourd´hui, dans les campagnes électorales, on valorise plus l´ image et la vie privée des politiciens que leurs idées. Dans cet article, j´analyse l’extrait d´une interview donnée par la ex-première ministre Marina Silva dans le talk show Jô 11/2. J´observe les indices verbaux et para-verbaux dans le discours de Mme Silva qui puissent indiquer com­ment elle cherche à contrôler ses émotions et à projeter son ethos en se basant sur une argumentation fondée sur le logos. Pour analyser la diversité pluri-sémiotique de ce discours, j´ai utilisé les concepts de modalisation et modulation selon Vion (1992; 2003. Je comprend le discours en tant qu´activité dialogique de production textuelle. Cette activité est determinée par de facteurs historiques, culturels, elle est produite aussi dans une situation de communication dans laquelle les sujets jouent de rôles sociaux, signalisent de positionnements, exercent une attitude active et responsive dans le processus communicatif: ils répondent à des énoncés anterieurs (les divers interdiscours et ils projetent ses allocutions en prévoyant des destinateurs potencials.

  11. The things they carried: The pathogenic effects of old and new parasites following the intercontinental invasion of the Australian cane toad (Rhinella marina

    Directory of Open Access Journals (Sweden)

    D. Selechnik

    2017-12-01

    Full Text Available Brought to Australia in 1935 to control agricultural pests (from French Guiana, via Martinique, Barbados, Jamaica, Puerto Rico and Hawai'i, repeated stepwise translocations of small numbers of founders enabled the cane toad (Rhinella marina to escape many parasites and pathogens from its native range. However, the infective organisms that survived the journey continue to affect the dynamics of the toad in its new environment. In Australia, the native-range lungworm Rhabdias pseudosphaerocephala decreases its host's cardiac capacity, as well as growth and survival, but not rate of dispersal. The lungworm is most prevalent in long-colonised areas within the toads' Australian range, and absent from the invasion front. Several parasites and pathogens of Australian taxa have host-shifted to cane toads in Australia; for example, invasion-front toads are susceptible to spinal arthritis caused by the soil bacterium, Ochrobactrum anthropi. The pentastome Raillietiella frenata has host-shifted to toads and may thereby expand its Australian range due to the continued range expansion of the invasive toads. Spill-over and spill-back of parasites may be detrimental to other host species; however, toads may also reduce parasite loads in native taxa by acting as terminal hosts. We review the impact of the toad's parasites and pathogens on the invasive anuran's biology in Australia, as well as collateral effects of toad-borne parasites and pathogens on other host species in Australia. Both novel and co-evolved pathogens and parasites may have played significant roles in shaping the rapid evolution of immune system responses in cane toads within their invaded range. Keywords: Rhabdias pseudosphaerocephala, Ecoimmunology, Invasion, Enemy release hypothesis, Immune function, Bufo, Pathogen-mediated selection 1

  12. Del “mar es de todos” al mar reservado: turistas, poblaciones de pescadores y reservas marinas en Canarias

    Directory of Open Access Journals (Sweden)

    José J. Pascual

    2003-01-01

    Full Text Available Los patrones de desarrollo de las últimas décadas en Canarias y en otras zonas del Estado han originado nuevos usos del espacio marítimo y litoral. Frente a los tradicionales vinculados a la pesca, ahora dominan frecuentemente los turísticos y recreativos. La naturaleza, construida o recreada, se convierte especialmente en los últimos años en un atractivo clave para muchos destinos. En este contexto, las reservas marinas intentan preservar ciertas zonas de especial valor biológico de los excesos de la pesca, a la vez que deberían conjugar los usos turísticos y recreativos a realizar en tales espacios con la conservación de sus valores naturales. En Canarias han constituido uno de los instrumentos claves de la política de conservación y gestión de pesquerías en las áreas costeras de las Islas. Actualmente hay tres en funcionamiento, siguiendo una tendencia cada vez más general en todo el mundo. Pero a la vez que con ellas se controla la extracción de recursos por los profesionales, se impulsan una serie de usos vinculados a determinadas formas de turismo que han generado importantes transformaciones en las poblaciones vinculadas a estas zonas protegidas. Otros impactos inducidos por el turismo o la acuicultura han alterado también sus estrategias económicas o el uso de los espacios tanto en tierra como en el mar

  13. Identification and analysis of red sea mangrove (Avicennia marina microRNAs by high-throughput sequencing and their association with stress responses.

    Directory of Open Access Journals (Sweden)

    Basel Khraiwesh

    Full Text Available Although RNA silencing has been studied primarily in model plants, advances in high-throughput sequencing technologies have enabled profiling of the small RNA components of many more plant species, providing insights into the ubiquity and conservatism of some miRNA-based regulatory mechanisms. Small RNAs of 20 to 24 nucleotides (nt are important regulators of gene transcript levels by either transcriptional or by posttranscriptional gene silencing, contributing to genome maintenance and controlling a variety of developmental and physiological processes. Here, we used deep sequencing and molecular methods to create an inventory of the small RNAs in the mangrove species, Avicennia marina. We identified 26 novel mangrove miRNAs and 193 conserved miRNAs belonging to 36 families. We determined that 2 of the novel miRNAs were produced from known miRNA precursors and 4 were likely to be species-specific by the criterion that we found no homologs in other plant species. We used qRT-PCR to analyze the expression of miRNAs and their target genes in different tissue sets and some demonstrated tissue-specific expression. Furthermore, we predicted potential targets of these putative miRNAs based on a sequence homology and experimentally validated through endonucleolytic cleavage assays. Our results suggested that expression profiles of miRNAs and their predicted targets could be useful in exploring the significance of the conservation patterns of plants, particularly in response to abiotic stress. Because of their well-developed abilities in this regard, mangroves and other extremophiles are excellent models for such exploration.

  14. Moving south: effects of water temperatures on the larval development of invasive cane toads (Rhinella marina) in cool-temperate Australia.

    Science.gov (United States)

    Wijethunga, Uditha; Greenlees, Matthew; Shine, Richard

    2016-10-01

    The distributional limits of many ectothermic species are set by thermal tolerances of early-developmental stages in the life history; embryos and larvae often are less able to buffer environmental variation than are conspecific adults. In pond-breeding amphibians, for example, cold water may constrain viability of eggs and larvae, even if adults can find suitable thermal conditions in terrestrial niches. Invasive species provide robust model systems for exploring these questions, because we can quantify thermal challenges at the expanding range edge (from field surveys) and larval responses to thermal conditions (in the laboratory). Our studies on invasive cane toads (Rhinella marina) at the southern (cool-climate) edge of their expanding range in Australia show that available ponds often average around 20°C during the breeding period, 10°C lower than in many areas of the toads' native range, or in the Australian tropics. Our laboratory experiments showed that cane toad eggs and larvae cannot develop successfully at 16°C, but hatching success and larval survival rates were higher at 20°C than in warmer conditions. Lower temperatures slowed growth rates, increasing the duration of tadpole life, but also increased metamorph body mass. Water temperature also influenced metamorph body shape (high temperatures reduced relative limb length, head width, and body mass) and locomotor performance (increased speed from intermediate temperatures, longer hops from high temperatures). In combination with previous studies, our data suggest that lower water temperatures may enhance rather than reduce recruitment of cane toads, at least in areas where pond temperatures reach or exceed 20°C. That condition is fulfilled over a wide area of southern Australia, suggesting that the continuing expansion of this invasive species is unlikely to be curtailed by the impacts of relatively low water temperatures on the viability of early life-history stages.

  15. Production of indoleacetic acid by strains of the epiphytic bacteria Neptunomonas spp. isolated from the red alga Pyropia yezoensis and the seagrass Zostera marina.

    Science.gov (United States)

    Matsuda, Ryuya; Handayani, Midia Lestari; Sasaki, Hiroyuki; Takechi, Katsuaki; Takano, Hiroyoshi; Takio, Susumu

    2017-10-10

    Neptunomonas sp. BPy-1 is an epiphytic bacterium isolated from in vitro culture of the red alga Pyropia yezoensis. It uses ethanol as a sole carbon source and promotes the growth of host alga. A related bacterium, Neptunomonas sp. BZm-1, was isolated from leaves of Zostera marina found in the Yatsushiro Sea (Japan). BZm-1 showed 99% 16S rRNA sequence identity with Neptunomonas sp. BPy-1. Similar to BPy-1, BZm-1 grew in artificial seawater (ASW) medium containing ethanol or butanol. When thalli were treated with a multi-enzyme cleaner, the growth of treated thalli was retarded, but the addition of BZm-1 to the medium promoted growth. To explore the benefits of epiphytic bacteria, indoleacetic acid (IAA) production by isolated bacteria was examined under conditions of limited nutrients. Salkowski assays and GC-MS analysis revealed that both BZm-1 and BPy-1 excreted IAA during growth in ASW medium containing glucose or ethanol in the presence of tryptophan. In ASW medium containing tryptophan but lacking a carbon source, neither isolate grow, but produced IAA. ASW medium includes nitrate as the sole nitrogen source. In the absence of carbon source, different nitrogen forms in the presence of tryptophan did not affect IAA production by the two isolates. These findings indicate that IAA production by the two isolates is strictly dependent on tryptophan but less affected by carbon and nitrogen sources. Based on the different origins of BPy-1 and BZm-1, this mode of IAA production seems to be conserved among relatives of BPy-1.

  16. Marina Núñez o la construcción de un cíborg. Un discurso multimedia entre la utopía y la distopía

    OpenAIRE

    Isabel Tejeda Martín

    2012-01-01

    El presente artículo se propone revisar, a partir de un número importante de entre los proyectos multimedia más importantes realizados en las dos últimas décadas por la artista española Marina Núñez Jiménez (Palencia, 1966), la construcción que ha llevado a cabo de alegorías sobre el concepto de cíborg, tanto en su sentido utópico como distópico. Se analiza, en este sentido, el reciclaje que esta...

  17. Comportamiento a la corrosión atmosférica marina de aceros autopatinables con estructura ferrítico perlítica y ferrítico martensítica

    OpenAIRE

    Artigas, Alfredo; Bustos, Oscar; Sipos, Konstantin; Garza-Montes-de-Oca, Nelson F.; Monsalve, Alberto; Mena, Juan; Seco, Rodrigo

    2015-01-01

    RESUMENLa industria del acero se encuentra constantemente enfrentada al desafío del desarrollo de nuevos y mejores aceros para diversas necesidades, tales como la necesidad de aceros estructurales con mayor resistencia a la corrosión marina que los que existen en la actualidad, y que además posean una mayor resistencia mecánica. Por ello y en pos de lo anteriormente expuesto es evidente que, investigar en forma sistemática tanto la influencia de los elementos aleantes, como las variables del ...

  18. Determinación de los factores que inciden en la captura incidental de aves marinas en la flota palangrera pelágica chilena Determination of factors affecting the bycatch of seabirds in Chilean pelagic longline fleet

    Directory of Open Access Journals (Sweden)

    Andrés González

    2012-09-01

    Full Text Available Se analizó la relación entre la variabilidad de la tasa de captura incidental de aves marinas en la pesquería palangrera pelágica de pez espada (Xiphias gladius y diversos factores temporales, ambientales, espaciales y pesqueros, entre 2007 y 2009. Los resultados muestran que las operaciones de pesca de esta flota afectan principalmente a los albatros, grupo que concentra el 83,7% de la captura incidental de aves marinas registrada en el período. Esta captura incidental se debe en primera instancia a la presencia del Frente Subtropical del Pacífico Sur, sistema que provoca la sobreposición entre la actividad de esta flota con la distribución espacial de aves marinas durante la fase residente del período no reproductivo. La variabilidad en esta captura incidental estaría fuertemente relacionada a los estímulos visuales (medido mediante el porcentaje de luces químicas y porcentaje de calamar y las condiciones de luminosidad ambiental existentes en los períodos de forrajeo diurno y nocturno de estas aves marinas durante el calado (medido a través del desfase de la hora del ocaso con respecto a la hora de inicio del calado y fase lunar.We analized the relationship between the variability in the rate of seabird bycatch in pelagic longline fishery for swordfish (Xiphias gladius, and several temporary, environmental, spatial and fisheries factors for the fishing period from 2007 to 2009. The results show that the fishing operations of this fleet mainly affect the albatross, group that accounts for 83.7% of the incidental catch of seabirds recorded in the period. This bycatch is due primarily to the presence of the South Pacific Subtropical Front, a system that causes the overlap between the activities of this fleet with the spatial distribution of these seabird species during the resident phase of the nonbreeding period. By the other hand, the variability in bycatch would be strongly linked to visual stimuli (measured by the percentage of

  19. AKUMULASI LOGAM BERAT Cr DAN Pb PADA TUMBUHAN MANGROVE AVICENNIA MARINA DI MUARA SUNGAI BABON PERBATASAN KOTA SEMARANG DAN KABUPATEN DEMAK JAWA TENGAH (Accumulation of Heavy Metals Cr and Pb in Mangrove Plant Avicennia marina On Babon River’s Estuari

    Directory of Open Access Journals (Sweden)

    Vita Kartikasari

    2002-11-01

    Full Text Available ABSTRAK Ekosistem mangrove cenderung dapat mengakumulasi unsur-unsur logam berat yang berada dalam perairan sekitar tumbuhan mengrove. Kajian ini dilakukan di muara sungai Babon, Semarang, Jawa Tengah. Tujuan penelitian untuk mengetahui kemampuan tumbuhan mangrove, Avicennia murina, dalam mengakumulasi unsur logam berat Cr dan Pb; mengetahui organ (akar, cabang, dan daun yang paling banyak mengakumulasi unsur logam berat dan mengetahui peran tumbuhan mangrove dalam mengurangi kandungan logam berat (Cr dan Pb yang ada di perairan muara sungai Babon. Penelitian ini dibagi menjadi dua tahap. Tahap observasi pendahuluan ditujukan untuk mengidentifikasi jenis-jenis tumbuhan yang hidup di ekosistem mangrove dan menentukan keberadaan unsur logam berat dalam organ tumbuhan mangrove. Setelah observasi pendahuluan dapat ditentukan lokasi sampling yang ada tumbuhan mangrove A. marina dan lokasi yang tidak ada tumbuhan tersebut. Penelitian utama ditujukan untuk memperoleh data primer konsentrasi Cr dan Pb dengan cara mencuplik dari organ tumbuhan (akar, cabang dan daun, sedimen dan air. Cuplikan dibawa ke laboratorium untuk dikeringkan dengan Microwave Digestion MLS-1200 MEGA dan ditentukan kadar Cr dan Pbnya. Data yang diperoleh kemudian dianalisis secara statistik Uji Non Parametric Kruskal Wallis dan Uji Two Test Kolmogorov Smimov. Hasil penelitian menampakkian Cr terakumulasi lebih banyak daripada Pb di tumbuhan mlangrove. Kecepatan faktor biokonsentrasi untuk Cr adalah 1052.66 dan Pb adalah 349.54. Tempat konsentrasi tertinggi Cr dan Pb dalam organ tumbuhan berturut-turut: akar, cabang dan dedaunan. Daun menyerap unsur Pb lebih besar daripada cabang. Hasil penelitian juga menunjukkan bahwa konsentrasi Cr dan Pb di sedimen yang perairannya ditumbuhi mangrove lebilr besar dari pada di sedimen yang perairannya tanpa ditumbuhi mangrove. Konsentrasi unsur logam berat dalam perairan yang ditumbuhi magrove.   ABSTRACT Mangrove have a tendency to accumulate

  20. Determinación paramétrica para el análisis de la viabilidad técnico económica de la implantación de un parque de aprovechamiento energético de corrientes marinas mediante un generador en inmersión con rotor de eje horizontal

    OpenAIRE

    Novoa Rojas, Eva Mª

    2016-01-01

    La presente Tesis se ha centrado en un análisis de la tecnología para la generación de energía eléctrica utilizando las corrientes marinas. El contenido desarrollado ha consistido en un análisis de las distintas alternativas para poder aprovechar la energía oceánica con especial interés en los desarrollos tecnológicos de las corrientes marinas. Posteriormente se ha descrito un nuevo prototipo, basado en un diseño de un generador en inmersión con rotor de eje horizontal con estructura en Y, de...

  1. Blood lead levels, δ-ALAD inhibition, and hemoglobin content in blood of giant toad (Rhinella marina) to assess lead exposure in three areas surrounding an industrial complex in Coatzacoalcos, Veracruz, Mexico.

    Science.gov (United States)

    Ilizaliturri-Hernández, César Arturo; González-Mille, Donaji Josefina; Mejía-Saavedra, Jesús; Espinosa-Reyes, Guillermo; Torres-Dosal, Arturo; Pérez-Maldonado, Iván

    2013-02-01

    The Coatzacoalcos Region in Veracruz, Mexico houses one of the most important industrial complexes in Mexico and Latin America. Lead is an ubiquitous environmental pollutant which represents a great risk to human health and ecosystems. Amphibian populations have been recognized as biomonitors of changes in environmental conditions. The purpose of this research is to measure exposure to lead and evaluate hematological and biochemical effects in specimens of giant toads (Rhinella marina) taken from three areas surrounding an industrial complex in the Coatzacoalcos River downstream. Lead levels in toads' blood are between 10.8 and 70.6 μg/dL and are significantly higher in industrial sites. We have found a significant decrease in the delta-aminolevulinic acid dehydratase (δ-ALAD) activity in blood from 35.3 to 78 % for the urban-industrial and industrial sites, respectively. In addition, we have identified a strong inverse relationship between the δ-ALAD activity and the blood lead levels (r = -0.84, p < 0.001). Hemoglobin and mean corpuscular hemoglobin levels, as well as the condition factor, are found to be lower at industrial sites compared with the reference sites. Our results suggest that the R. marina can be considered a good biomonitor of the δ-ALAD activity inhibition and hematological alterations at low lead concentrations.

  2. Biophysical characterisation of GlycoPEGylated recombinant human factor VIIa

    DEFF Research Database (Denmark)

    Plesner, Bitten; Westh, Peter; Nielsen, Anders D.

    2011-01-01

    The effects of GlycoPEGylation on the structural, kinetic and thermal stability of recombinant human FVIIa were investigated using rFVIIa and linear 10 kDa and branched 40 kDa GlycoPEGylated® recombinant human FVIIa derivatives. The secondary and tertiary structure of rFVIIa measured by circular...

  3. Kuhu riiki oma firma masust taastuma viia? / Villi Tõntson

    Index Scriptorium Estoniae

    Tõntson, Villi, 1975-

    2010-01-01

    PricewaterhouseCoopersi ja Maailmapanga koostöös valminud maksukeskkondade võrdlevast uuringust "Paying Taxes 2010", kus üks hüpoteetiline ettevõte paigutati eri riikidesse ning seejärel reastati riigid vastavalt ettevõtte maksukoormusele, riiklike ja kohalike maksude hulgale ning maksude maksmiseks kulunud ajale. Graafik

  4. Juhan Parts soovib Res Publica tagasi tippu viia / Toomas Sildam

    Index Scriptorium Estoniae

    Sildam, Toomas, 1961-

    2004-01-01

    Res Publica esimees peaminister Juhan Parts plaanib minna oma erakonnaga kohalikele valimistele ning uurib võimalusi paremerakondade ühinemiseks. Rakveres toimunud erakonna üldkogust. Lisad: Peaministri neli rasket hetke; Res Publica ministrid said parteilt toetuse. Vt. samas: Reformierakond kiitis Res Publicat hea koostöö eest; Parts: 191 vastuhäält on hea tulemus

  5. Activity of recombinant factor VIIa under different conditions in vitro

    DEFF Research Database (Denmark)

    Bladbjerg, Else-Marie; Jespersen, Jørgen

    2008-01-01

    investigated the in-vitro effects of pH, temperature, and haemodilution on the activity of recombinant activated factor VII. Samples from eight healthy volunteers were spiked with recombinant activated factor VII (final concentration 1.7 microg/ml) and adjusted to pH 6.0, 6.5, 7.0, and 7.4 or analysed at 30...... activity in plasma. Significant effects of pH were observed for clotting time, clot formation time, maximum clot firmness, and factor VII coagulant activity in the direction of longer clot formation times and less firm clots with decreasing pH. Temperature had significant effects on clotting time, clot......, but no effects on clotting time indicating that haemodilution does not affect clot formation, but the clot formed at high haemodilution may not be so firm. In conclusion, the activity of recombinant activated factor VII was affected in vitro by pH, temperature, and haemodilution. Additional studies are necessary...

  6. ASPECTOS POBLACIONALES Y ECOLÓGICOS DE PECES DEMERSALES DE LA ZONA NORTE DEL CARIBE COLOMBIANO EN RELACIÓN CON EL HÁBITAT: UNA HERRAMIENTA PARA IDENTIFICAR ÁREAS MARINAS PROTEGIDAS (AMPs PARA EL MANEJO PESQUERO

    Directory of Open Access Journals (Sweden)

    Paramo Jorge

    2009-06-01

    Full Text Available Las pesquerías marino-costeras están bajo amenaza de colapso debido al incrementodel esfuerzo pesquero y los impactos antropogénicos que directa e indirectamentedegradan el hábitat y los sistemas ecológicos de los cuales depende la actividadpesquera. La pesca acompañante (bycatch y los descartes globales equivalen a untercio (1/3 de la biomasa total desembarcada. Particularmente, el bycatch de lapesca de arrastre de camarón en aguas tropicales representa el 27 % de todos losdescartes globales, lo cual para el manejo de las pesquerías es un serio problemaecológico. Por lo tanto, hay una necesidad de medidas de manejo adicionales a lasusadas tradicionalmente, que contribuyan a proteger los recursos pesqueros y mejorarla producción pesquera. Las Áreas Marinas Protegidas (AMPs han emergido comouna herramienta para la conservación marina y el manejo de pesquerías. El propósitodel trabajo es identifi car potenciales AMPs para el manejo de los recursos pesqueros 124demersales mediante un análisis de la relación entre la distribución espacial deaspectos poblacionales y ecológicos de estos recursos en la zona norte del Caribecolombiano con las características del hábitat (temperatura, salinidad, profundidad,tipo de sedimento. Se estableció la relación de la estructura poblacional de los pecesdemersales con las condiciones del hábitat, que resultó importante cuando se quiereidentifi car AMPs como una herramienta de manejo pesquero sostenible bajo unenfoque ecosistémico. Mediante modelación espacial se calculó el área de distribuciónespacial de los peces demersales (5805.8 km2 y de ésta, los juveniles se encontraronentre Dibulla y el Río Buritaca en un área de 393.1 km2 y en La Guajira se encontróel área de mayor diversidad 297.0 km2, las cuales representan un 11.9 % del área dedistribución espacial de los peces demersales. Se plantea la implementación de ÁreasMarinas Protegidas de Múltiples Usos (AMP-MU en estos

  7. Adaptive management of marine protected areas in Chile: a method for his evaluation Gestión adaptativa en áreas marinas protegidas de Chile: un método para su evaluación

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    Andrés Guajardo

    2012-09-01

    Full Text Available The evaluation of the performance of the management is essential to fulfill the objectives in a Marine Protected Area (MPA. Therefore, Robert Pomeroy in 2004 presents a manual to evaluate the development of the marine protected areas through time, by using of indicators which measure the biophysical state, socioeconomic state and governance. The present work adopts the use of those indicators to evaluate the performance of the management in the Marine Reserve Chanaral Island, Atacama region. The results of the evaluation show a partial level of protection objectives fulfillment, suggesting the lack of efficient management of the reserve to ensure its suitable development. This work also presented a descriptive model, that interprets the behavior that area would facein front of two opposed scenarios, and the expected due to inefficient management of the area and otherwise an adaptive management.La evaluación del desempeno en la gestión es esencial para el eventual logro de los objetivos de las áreas marinas protegidas (AMP. Robert Pomeroy en 2004 presenta un manual para evaluar el desarrollo de las áreas marinas protegidas en el tiempo, por medio de la medición de indicadores de estado: biofísicos, socioeconómicos y de gobernabilidad. El presente trabajo adopta el uso de estos indicadores, para evaluar el desempeno en la gestión de la Reserva Marina Isla Chanaral, región de Atacama. Los resultados de su evaluación muestran que se cumplen parcialmente los objetivos de protección, sugiriendo la falta de una gestión efectiva que asegure el adecuado desarrollo de la reserva. En el trabajo también se presenta un modelo descriptivo que interpreta el comportamiento que tendría el área frente a dos escenarios opuestos y los resultados esperados a causa de una gestión ineficiente del área y en caso contrario una gestión adaptativa.

  8. 6a metsaretkel / Marina Poltavtseva

    Index Scriptorium Estoniae

    Poltavtseva, Marina

    2011-01-01

    Ühelt õppekäigult Tädu loodusõpperajal Viimsi vallas Randvere tee ääres Tallinna Laagna Gümnaasiumi 6a klassi õpilastega. Retkejuht oli Tallinna Ülikoolis magistrantuuris keskkonnakorraldust õppiv Daniel Juhhov

  9. Patrimonio Natural y Reservas Marinas

    Directory of Open Access Journals (Sweden)

    Cruz Modino, Raquel de la

    2004-01-01

    Full Text Available Marine reserves try to preserve some areas, with a special biologic interest, to the fish excess for to secure the protection, the regeneration and the marine recourses development. With them it is trying to regulate the different activities that are realized on these areas, and to conjugate tourist and leisure uses with the conservation their natural values. Perhaps, generality the regulation over uses that can to be developed on natural and fragile areas like these is restricted to establish limitations over the activities developed on the area. The creation of figures like this, on a tourist context, may to promote the realisation of a recreational activities in front of the traditional uses that can to stay limited in the protected area

  10. Los colores de las conchas marinas en el antiguo occidente de México. El caso del Posclásico Seashell color in ancient western Mexico. The case of the Postclassic

    Directory of Open Access Journals (Sweden)

    Luis Gómez-Gastélum

    2007-10-01

    Full Text Available A partir de los estudios sobre la cosmovisión mesoamericana -en especial aquellos relacionados con el cuerpo humano y las significaciones del color-, se analizan los patrones de uso de las conchas marinas y los objetos elaborados con éstas en las sociedades prehispánicas del occidente de México durante el periodo Posclásico (1100-1350 d.C., con la finalidad de acercarse al simbolismo que les fueron otorgados por parte de quienes las utilizaron.Based on studies of Mesoamerican cosmology, especially with regard to the human body and the meaning of color, we analyzed the use patterns of seashells and objects made from seashells in the Pre-Hispanic societies of western Mexico in the Postclassic period (1100-1530 A.D.. The goal was to understand the symbolism given to these objects by the people who utilized them.

  11. Artistas madrileños de comienzos del seiscientos en la Baja Extremadura : su intervención en el ornato litúrgico-funeraho de la iglesia conventual de Santa Marina de Zafra

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    Juan Carlos Rubio Masa

    1994-01-01

    Full Text Available Zafra, durante el último tercio del siglo XVI y el primero del XVII, merced a un ambicioso programa de mecenazgo, fue transformándose en una villa cuya apariencia debía ser un reflejo del prestigio político y de la solvencia económica de los Suárez de Figueroa, duques de Feria y señores del lugar. Hasta 1612, la dignidad ducal la ostentó doña Juana Dormer (Milora Dormer Inglesa, como era conocida en la Corte, esposa de don Gomes Suárez de Figueroa, primer duque de Feria. Su larga vida le permitirá conocer tres generaciones del linaje, sobre las que impondrá su personalidad: su temprana viudez en 1571, el testamento de su marido, la niñez del nuevo duque y el posterior absentismo de su hijo y de su nieto, ocupados en asuntos de Estado fuera de Castilla, dotarán a doña Juana de una importantísima capacidad de decisión sobre la gobernación del estado de Feria y, como consecuencia, sobre las fábricas y obras que se proyectaron. Una de las empresas que se acometen será la remodelación y ampliación del alcázar medieval para convertirlo en un palacio. Para nuestro tema son fundamentales las obras realizadas entre 1605 y 1609, consistentes en la edificación de nueva planta de una galería o pasadizo para unir el palacio a la vecina iglesia de Santa Marina, que se reconstruyó simultáneamente. En esta fase de la remodelación tuvo un especial papel el legado testamentario de doña Margarita Harrington al convento de clarisas de Santa Marina.

  12. Bibliografía sobre investigaciones marinas, oceanográficas, geológicas y atmosféricas en el Parque Nacional Isla del Coco y aguas adyacentes, Pacífico de Costa Rica

    Directory of Open Access Journals (Sweden)

    Jorge Cortés

    2012-11-01

    Full Text Available Isla del Coco, Pacífico de Costa Rica, ha sido visitada y se ha escrito sobre ella desde el Siglo XVI. Desde finales del Siglo XIX muchas expediciones han desarrollado investigaciones en el Isla. Aquí compilo las publicaciones sobre biología y geología marina, oceanografía física y química, ciencias atmosféricas y algunos temas sociales. Una lista de 599 artículos y capítulos, 41 libros, Suplementos, tesis, informes y un sitio de internet es incluida, y diez libros históricos donde se describen organismos y ambientes marinos de la Isla del Coco. La mayoría de las publicaciones (425 o 66.4% son sobre biodiversidad marina (descripciones, listas y distribuciones de especies. De los grupos que más publicaciones hay son de los gasterópodos (81 trabajos, decápodos (67 y peces óseos (66. No hay publicaciones de varios grupos de animales que han sido observados en Isla del Coco, por ejemplo, gusanos planos de vida libre, parásitos o nemertinos, entre otros. Temas con muchas publicaciones son: biogeografía con 65, ecología (61 y geología (48; los ecosistemas más estudiados son los arrecifes coralinos con 14 trabajos. Se necesitan más publicaciones en muchos tópicos, tales como el impacto sobre la flora y fauna del Parque Nacional Isla del Coco de la pesca ilegal y el cambio climático.

  13. Marina Núñez o la construcción de un cíborg. Un discurso multimedia entre la utopía y la distopía

    Directory of Open Access Journals (Sweden)

    Isabel Tejeda Martín

    2012-04-01

    Full Text Available El presente artículo se propone revisar, a partir de un número importante de entre los proyectos multimedia más importantes realizados en las dos últimas décadas por la artista española Marina Núñez Jiménez (Palencia, 1966, la construcción que ha llevado a cabo de alegorías sobre el concepto de cíborg, tanto en su sentido utópico como distópico. Se analiza, en este sentido, el reciclaje que esta artista hace en su trabajo con los referentes religiosos católicos de la historia del arte, estudiando en concreto su intervención Carne para la sala de exposiciones de Verónicas, un espacio connotado de arquitectura barroca que en su tiempo fue una iglesia conventual.Nos basaremos en sus exposiciones entendidas como site-specific, en sus instalaciones, cajas de luz, vídeos y fotografías, imágenes que serán analizadas desde una perspectiva iconográfica, técnica y lingüística casi en su totalidad. Asimismo, estos trabajos se ponen en relación con los referentes críticos más importantes de la autora, Michel Foucault, Donna Haraway, Rosi Braidotti, etc.El artículo traza un recorrido por la literatura crítica existente sobre esta Marina Núñez aparecida tanto en revistas especializadas como en forma de textos de catálogos individuales, al tiempo que refiere sus profundas relaciones formales e iconográficas con la literatura Cíberpunk y con el cine de ciencia ficción.

  14. Impacto de la luz artificial sobre la anidación de la tortuga marina Dermochelys coriacea (Testudines: Dermochelyidae, en playa Cipara, Venezuela

    Directory of Open Access Journals (Sweden)

    María Rondón Médicci

    2009-09-01

    Full Text Available En Playa Cipara, Península de Paria, Venezuela, se evaluó el impacto de la luz artificial sobre la anidación de la tortuga cardón. Se estimó el número de anidaciones y su distribución espacial a lo largo de la playa entre los años sin y con iluminación artificial y entre segmentos de playa iluminada y oscura. Se hicieron entrevistas a los residentes para conocer su percepción sobre el impacto de la luz artificial hacia las tortugas marinas. Entre el 2000 y 2005 se registraron 1 217 salidas de tortuga cardón; con 1 056 nidos. El número de nidos con huevos dependió significativamente del año (p=0.035 al igual que el número de nidos totales (p=0.015. En los años previos a la electricidad (2000-2003 se observaron 743 salidas, de las cuales 661 con nido y 374 nidadas confirmadas. En los dos años (2004-2005 con iluminación artificial, se contaron 474 salidas con 395 nidos y 232 nidadas. La proporción de salidas con construcción de nido disminuyó significativamente (p=0.005 en los años con luz eléctrica, pero no varió el éxito de desove (p=0.402. No se encontró diferencia significativa entre el número de salidas por metro de playa en los sectores oscuros y los iluminados (p=0.244, ni entre el número de nidos construidos (p=0.379, ni entre las anidaciones con desove (p=0.516. Tampoco en la proporción de las salidas totales que constituyeron anidaciones (p=0.067 entre los sectores iluminados y oscuros, ni en la proporción de nidos exitosos (p=0.833. El volumen medio de arena por metro de playa fue mayor en La Peña, Cipara y La Remate y menor en Varadero (pImpact of artificial light on nesting in the leatherback turtle Dermochelys coriacea (Testudines: Dermochelyidae at Cipara beach, Venezuela. The number of Leatherback turtle nests and their spatial distribution was compared between years with and without artificial light, and between dark and lighted beach segments, in Cipara Beach, Paria Peninsula, Venezuela. Residents

  15. El manejo del área marina y costera protegida Actam Chuleb y los beneficios económicos que genera a los usuarios del municipio de San Felipe, Yucatán, México

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    Wilian de Jesús Aguilar Cordero

    2012-01-01

    Full Text Available El área marina y costera protegida (AMyCP Actam Chuleb se ubica en la costa oriente del estado de Yucatán, en el municipio de San Felipe, donde se realizó el estudio con el fin de caracterizar el manejo de los recursos naturales y estimar los beneficios de uso directo. La metodología se desarrolló en dos etapas; en la primera se identificaron y analizaron las prácticas de manejo y conservación, los bienes y los principales usuarios por medio de entrevistas semiestructuradas y un diagnóstico participativo; en la segunda etapa, se estimó el valor de los beneficios económicos de uso directo y su relevancia en la estructura de actividades económicas de la comunidad por medio una encuesta a los usuarios directos. Los resultados mostraron que no existe un plan de manejo comunitario, sólo se cuenta con una normativa que no es cumplida a cabalidad por la comunidad y que hay una falta de conocimientos, participación y comunicación de los actores sociales para el manejo adecuado y una correcta toma de decisiones. Existe una marcada centralización de poderes, además, los usuarios perciben con mayor facilidad los beneficios de uso directo, generados por la pesca y el turismo, debido a su tangibilidad.

  16. EL JUEGO: UNA ESTRATEGIA PARA LA ENSEÑANZA-APRENDIZAJE EN AMBIENTES VIRTUALES DE LOS SUBOFICIALES DE LA ESCUELA DE FORMACIÓN DE INFANTERÍA DE MARINA DE COVEÑAS SUCRE, COLOMBIA

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    Freddy Anderson Cortés otalora

    2016-08-01

    Full Text Available El juego adquiere especial relevancia en la vida de los seres humanos en la medida que ayuda a formar nuestra identidad, personalidad y a fortalecer los lazos sociales aceptados y convencionalizados por la cultura. En este sentido, el propósito de este artículo es analizar como el juego trasciende su significación meramente lúdica y de ocio para convertirse en una herramienta que resulta útil en la enseñanza-aprendizaje tradicional, pero también en los ambientes o entornos virtuales de aprendizaje. De ahí que en la primera parte, el planteamiento del problema del juego en relación con la educación se haga explícito. El artículo también se centra en presentar algunos antecedentes del juego y su valor pragmático en la educación, conectado con la didáctica, la pedagogía y las teorías del juego. Finalmente, el artículo señala la utilidad que adquieren los videojuegos en el proceso de enseñanza-aprendizaje en ambientes virtuales para la formación de los suboficiales de la Escuela de Formación de Infantería de Marina de Coveñas, en el departamento de Sucre (Colombia.

  17. ANALISA ELECTRODE CONSUMABLE TYPE OK AUTROD 12.10 PENGELASAN SUBMERGED ARC WELDING PADA BLOK-BLOK KAPAL DCV 18500 DWT DI PT. JASA MARINA INDAH UNIT II SEMARANG

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    Sukanto Jatmiko

    2012-07-01

    Full Text Available In this globalization era technological advances growed very fast. In shipping industry of development process shipbuilding of principal feedstock to used steel plate, with construction tacking on using welder method.In this research purpose of lifted is know number of requirement (consumable electrode for welder SAW at block-block ship DCV 18500 DWT in PT. Jasa Marina Indah Semarang.At this experiment specimen applied is low carbon steel of type ST 42 with thickness of 12, 13, 14, 17, 19 dan 24 mm. Research is done by the way of making specimen at every plate thickness. Then is done path measurement of length, used electrode length, and weight flux applied at the welder.From result of gauging and data calculation welder at block DB 5(p/c/s, SS5A(p/s, SS 5B(p/s, UD 5C, and TB 102 (p/c/s will be known number of electrodes applied in welder SAW and number of flux used.

  18. Parámetros reproductivos y poblacionales de Thais chocolata (Duelos, 1832 (Gastropoda, Thaididae, en la reserva marina La Rinconada, Antofagasta, Chile Reproductive and population parameters of Thais chocolata (Duclos, 1832 (Gastropoda, Thaididae in La Rinconada marine reserve, Antofagasta, Chile

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    Marcela Cantillánez

    2011-11-01

    Full Text Available Para obtener antecedentes reproductivos y poblacionales de Thais chocolata que contribuyan a validar su actual normativa pesquera, se realizó un estudio en el área protegida de la reserva marina La Rinconada, Antofagasta, Chile, entre diciembre 2008 y enero 2010. Los resultados obtenidos indicaron a nivel reproductivo, que el desarrollo gonadal de la población es asincrónico, encontrándose ejemplares en diferentes etapas de maduración durante el año. Los individuos maduros se estratificaron entre 5 y 13 m de profundidad, y gran parte del año formaron agregaciones a 5 m de profundidad. Se determinaron períodos de mayor madurez en julio-agosto, y en noviembre-enero, manifestándose las agregaciones más importantes al final de ellos. Una relación se observó entre meses de mayor madurez y registro de agregaciones, con aquellos de mayor variación intradiaria de temperatura. A nivel poblacional los resultados permitieron estimar una población de 2,3*10(6 ejemplares, donde el 39% se encontró sobre la talla mínima legal (TML = 55 mm. Los parámetros de crecimiento mostraron crecimiento relativamente lento, que podría estar influenciado por la alta variabilidad que presenta la temperatura de fondo en este sector. Mientras que su talla crítica, y la talla de primera madurez sexual poblacional, resultaron ser mayores a la TML. Se determinó la necesidad de revisar la normativa pesquera actual de esta especie, y se demostró la efectividad de las reservas marinas propiciadas por el Estado en la conservación de los recursos marinos.Reproductive and population parameters of Thais chocolata that would contribute to the validation of the current extraction standards were obtained by performing a study in the protected area of La Rinconada Marine Reserve, Antofagasta, Chile, from December 2008 to January 2010. In terms of reproduction, the results revealed asynchronic gonad development in the population, with specimens in different stages of

  19. Captura incidental de tortugas marinas durante El Niño 1997-1998, en el norte del Perú Sea turtles by-catch during El Niño 1997-1998, in northern Perú

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    Javier Castro

    2012-11-01

    Full Text Available Se presenta evidencia del aumento de capturas incidentales de tortugas marinas en el norte del Perú, durante el fenómeno El Niño Oscilación del Sur (ENOS 1997-1998. El área de estudio se ubica frente a Lambayeque, entre 6°20'S y 7°10'S, y desde la costa hasta 35 mn mar afuera. Se analizaron y describieron los aparejos de enmalle por ser los que más interactuaban con estas tortugas, así como las características de las embarcaciones. Se registraron las tortugas capturadas por la flota artesanal entre enero 1996 y diciembre 1998; se identificó las especies capturadas y se analizó la captura por unidad de esfuerzo (CPUE; la información se correlacionó con la temperatura superficial del mar (TSM. Se analizó un total de 265 operaciones de pesca, capturándose un total de 383 tortugas, correspondiendo 80,4% a la tortuga pico de loro (Lepidochelys olivacea, 19,3% a la tortuga verde (Chelonia mydas y 0.2% a la tortuga carey (Eretmochelys imbricata. Se encontró una correlación altamente significativa entre las capturas de tortugas marinas y la TSM con un intervalo de confianza del 99% (Pearson; r = 0,787; σ = 0,000; N = 36. Se recomienda reforzar la colaboración entre entidades públicas y privadas para implementar medidas de manejo adecuadas para la conservación de estas especies amenazadas, sobre todo ante la eventualidad de un fenómeno ENOS.The main purpose of this work is to present evidence of sea turtles by-catch increase in northern Peru during the 1997-1998 El Niño Southern Oscillation (ENSO process. The study area is located off Lambayeque, between 6°20'S and 7°10'S, and from the coastline up to 35 nm offshore. The gillnet artisanal fishery was analyzed and described, since this was the fishing gear which most interact with sea turtles, the boat characteristics were evaluated as well. Sea turtle captures and species identification were registered from January 1996 until December 1998. The catch per unit effort (CPUE was

  20. Efecto de la radiación ultravioleta B en la producción de polifenoles en la microalga marina Chlorella sp. Effect of ultraviolet B radiation on the production of polyphenols in the marine microalga Chlorella sp.

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    Jaime Copia

    2012-03-01

    Full Text Available Las algas marinas son una fuente importante de compuestos antioxidantes (fenoles y polifenoles, generados como mecanismos de defensa frente a factores de estrés (radiación UV, temperatura, herbívora. El objetivo de este trabajo es evaluar la estrategia de adaptación al efecto de la radiación ultravioleta B (RUV-B, 280-315 nm en la microalga marina Chlorella sp. mediante la producción de polifenoles y capacidad antioxidante total. Se expusieron cultivos de Chlorella sp. fueron expuestos a radiación UV-B (470 μW cm-2 por periodo de tiempos ascendentes. Se evaluó la capacidad antioxidante total DPPH, polifenoles totales, clorofila-a y b así como la densidad celular en cultivos expuestos y no expuestos. Los resultados indicaron que la RUV-B genera una disminución de la densidad celular en los cultivos irradiados por primera vez (1ª etapa, existiendo un aumento significativo (P Marine algae are an important source of antioxidant compounds (phenols and polyphenols, generated as defense mechanisms against stress factors (UV radiation, temperature, herbivory. The aim of this study was to evaluate the strategy of adaptation to the effect of ultraviolet B radiation (UV-B, 280-315 nm in the marine microalga Chlorella sp. through, the production of polyphenols and total antioxidant capacity. Chlorella sp. cultures were exposed to UV-B radiation (470 μW cm-2 over increasing time periods. We evaluated the total antioxidant capacity DPPH, total polyphenols, chlorophyll-a and b, and cell densities in exposed and unexposed cultures. The results indicated that UV-B caused a decrease in cell density in cultures irradiated for the first time (1st stage, with a significant increment (P < 0.05, lower than the control in the 2nd and 3rd stages only through the 4th stage (day 7, corresponding to a dose of 16,920 J m-2. The production of total phenols increased significantly (P < 0.05 for the IVth extract with respect to the control, confirming that the

  1. Stress proteins and auxiliary anti-stress compounds in intertidal macroalgae Proteínas de estrés y compuestos anti-estrés auxiliares en algas marinas intermareales

    Directory of Open Access Journals (Sweden)

    Edgardo Cruces

    2012-11-01

    Full Text Available Intertidal macroalgae are exposed to strong variation in the physical environment and thus, diverse anti-stress mechanisms are displayed by these organisms. Stress proteins (also called heat shock proteins, HSPs have been invoked as potential protective mechanism, especially during stressful action of temperature and solar radiation. Therefore, macroalgae have not normally been used as model organisms in studies of these molecules. The present study compiles the existing information from intertidal species in the context of major factors that have been reported to induce them, e.g. temperature, enhanced solar radiation, contaminants, etc. Additionally, in order to address the question whether the expression of these proteins operates in intertidal macroalgae complementarily with other protective mechanisms, a case study of induction of HSPs after exposure to UV radiation and high temperature in two upper littoral species, Ulva sp. and Porphyra columbina, from southern Chile is presented. In parallel, two well-known responses to stress, photoinhibition of photochemical reactions (Fv/Fm and ROS scavenging were measured. The results indicated that, although stress proteins were detected in a time span between 3 and 24 h, the responses were not correlated with photochemical and antioxidative response. Overall, the study outlines a potential role of stress proteins in ecophysiological responses developed to cope mainly with high temperature and UV radiation. However, other rapid metabolic adjustments (e.g. high thermo-tolerance of photosynthesis and efficient ROS scavenging, together with other biomolecules (mycosporines, phenols, polyamines, etc. and morpho-functional adaptations to the intertidal life (e.g. small size, high area/volume ratio are also important.Las macroalgas marinas intermareales están expuestas a extrema variación en las condiciones ambientales y por ello desarrollan una serie de mecanismos anti-estrés. Las proteínas de estr

  2. De Las Vegas a Marina D'or. O como llegar desde el New Jornalism norteamericano de Hunter S. Thompson hasta la nueva narrativa española de Robert Juan-Cantavella

    Directory of Open Access Journals (Sweden)

    María Angulo Egea

    2011-12-01

    Full Text Available The north-american trend called 'New Journalism' born in the 60's, was a revolution for the USA traditional Journalism, for its use of literary elements with other ones that belong to the journalistic research. Hunter S. Thompson, author of Fear and loathing in Las Vegas (1971, with his gonzo style, was going to shed new light on the New Journalism proposals, improving the immersión process using drugs until he gets implied personally in his reports and taking all his narrative influences from the 50's beat movement. Almost 35 years later, in El Dorado, Juan-Cantavella makes a paralellism between Las Vegas and the holiday city in the mediterranean coast, Marina D'Or and he develops in this space a novel, where his alter ego, the punk journalist Escargot, plays the lead. Novel, journalism, gonzo, fiction, story, punk, essay, report, 'aportaje', discursive fragmentation, dialogs, internal monologues, descriptions, memorandums, telegrams, emails, press releases, radio news, songs, slogans, all fits to reenact genres, to re-read journalistic and literary traditions and to build something new. This work analyses this process, that goes from the gonzo journalism of Fear and loathing in Las Vegas to the current Spanish narrative of El Dorado. It tries to distinguish the literary resources that the gonzo journalist uses to build his reports and the journalistic tools that nourish Juan-Cantavella's 'punk narrative'. A particular example of literary-journalistic symbiosisLa corriente norteamericana llamada 'Nuevo periodismo', nacida en los años sesenta, supuso para la Periodística tradicional de Estados Unidos toda una revolución por la combinación de elementos literarios con otros propios de la investigación periodística. Hunter S. Thompson, autor de Miedo y asco en las Vegas (1971, con su periodismo gonzo, le iba a dar una vuelta más a la propuesta del New Journalism, llevando al extremo el proceso de inmersión, hasta implicarse personalmente

  3. Aplicación de la Tecnología SIG para la representación de información climática marina en el Atlántico Sur Application Of Gis Technology For The Representation Of Climate Marine Information In The South Atlantic Region

    Directory of Open Access Journals (Sweden)

    Elisa C. Nuré

    2011-06-01

    Full Text Available Este trabajo presenta una aplicación de la tecnología de Sistemas de Información Geográfica (SIG a las bases de datos de información meteorológica marina generadas en función de procedimientos estadísticos recomendados por la Organización Meteorológica Mundial para la elaboración de Resúmenes Climáticos Marinos. Este SIG permite una visualización dinámica de valores extremos y frecuencias en forma de mapas, tablas y gráficos, tanto temporales como espaciales, así como de la distribución de las observaciones meteorológicas marinas en el Océano Atlántico Sudoccidental. La información climática regional marina elaborada utilizando esta tecnología, es de interés para toda ciencia o disciplina relacionada con el medio ambiente y constituye un valioso soporte para la planificación de tareas en el ámbito de la seguridad náutica, tanto para el Servicio de Hidrografía Naval como para otras instituciones que desarrollen actividades afines.This article shows a Geographic Information Systems (GIS application to marine climatic information data bases. These data bases are created following the World Meteorological Organization recommendations for applying statistical procedures to marine observations in order to elaborate Marine Climatic Summaries. This GIS allows a dynamic visualization of maps, tables and graphics showing extreme values and frequencies of meteorological variables in time and space as well as the marine meteorological observations distribution over the South Atlantic Ocean. The regional climatic marine information obtained using this technology, will be a useful tool for environmental related disciplines and, it will be also of valuable support for the safety of naval operations.

  4. Análisis del desarrollo de las competencias básicas en el currículum de la Educación Física en la ESO en la Marina Baixa. Un estudio de caso

    Directory of Open Access Journals (Sweden)

    Julio Barrachina Peris

    2012-12-01

    Full Text Available Con la aprobación de la Ley Orgánica de Educación (LOE, 2006 las competencias básicas cobran un especial protagonismo en la enseñanza. Existe poco conocimiento sobre su desarrollo práctico en el currículum de la educación física en secundaria. El presente artículo resume una investigación realizada en el curso 2010 en los IES de la comarca de La Marina Baixa (Alicante. Enmarcada en la metodología cualitativa, se presenta un estudio de caso sobre una muestra de 28 profesores, con el objetivo de averiguar qué percepción tienen de éstas, qué estrategias emplean para integrarlas en la programación y cuáles son los principales obstáculos con que se encuentran para materializarlas en la práctica. Los datos se analizaron con el programa Aquad 6 y los resultados evidencian que el enfoque por competencias aún está consolidándose entre el profesorado. Las principales estrategias empleadas para su desarrollo se relacionan con la toma de decisiones en el diseño curricular, seguidas por la promoción de la participación y motivación del alumnado. En cuanto a los obstáculos, destacan la escasez y precariedad de los recursos disponibles, la falta de información y formación recibida por el profesorado, así como la escasa implicación de profesores, padres y alumnos.

  5. Exploiting COSMO-Skymed Data and Multi-Temporal Interferometry for Early Detection of Landslide Hazard: A Case of Slope Failure and Train Derailment Near Marina Di Andora, Italy.

    Science.gov (United States)

    Wasowski, J.; Chiaradia, M.; Bovenga, F.; Nutricato, R.; Nitti, D. O.; Milillo, G.; Guerriero, L.

    2014-12-01

    The improving temporal and spatial resolutions of new generation space-borne X-Band SAR sensors such as COSMO-SkyMed (CSK) constellation, and therefore their better monitoring capabilities, will guarantee increasing and more efficient use of multi-temporal interferometry (MTI) in landslide investigations. Thanks to their finer spatial resolution with respect to C-band data, X-band InSAR applications are very promising also for monitoring smaller landslides and single engineering structures sited on potentially unstable slopes. This work is focused on the detection of precursory signals of an impending slope failure from MTI time series of ground deformations obtained by exploiting 3 m resolution CSK data. We show the case of retrospectively captured pre-failure strains related to the landslide which occurred on January 2014 close to the town of Marina di Andora. The landslide caused the derailment of a train and the interruption of the railway line connecting north-western Italy to France. A dataset of 56 images acquired in STRIPMAP HIMAGE mode by CSK constellation from October 2008 to May 2014 was processed through SPINUA algorithm to derive the ground surface deformation map and the time series of displacement rates for each coherent radar target. We show that a cluster of moving targets coincides with the structures (buildings and terraces) affected by the 2014 landslide. The analysis of the MTI time series further shows that the targets had been moving since 2009, and thus could have provided a forewarning signal about ongoing slope or engineering structure instability. Although temporal landslide prediction remains difficult even via in situ monitoring, the presented case study indicates that MTI relying on high resolution radars such as CSK can provide very useful information for slope hazard mapping and possibly for early warning. Acknowledgments DIF provided contribution to data analysis within the framework of CAR-SLIDE project funded by MIUR (PON01_00536).

  6. Feasibility Study of Economics and Performance of Solar Photovoltaics at the Former Fort Ord Army Base Site in Marina, California. A Study Prepared in Partnership with the Environmental Protection Agency for the RE-Powering America's Land Initiative: Siting Renewable Energy on Potentially Contaminated Land and Mine Sites

    Energy Technology Data Exchange (ETDEWEB)

    Stoltenberg, B.; Konz, C.; Mosey, G.

    2013-05-01

    The U.S. Environmental Protection Agency (EPA), in accordance with the RE-Powering America's Land initiative, selected the Former Fort Ord Army Base (FOAB) site in Marina, California, for a feasibility study of renewable energy production. The National Renewable Energy Laboratory (NREL) provided technical assistance for this project. The purpose of this report is to assess the site for a possible photovoltaic (PV) system installation and estimate the cost, performance, and site impacts of different PV options. In addition, the report recommends financing options that could assist in the implementation of a PV system at the site.

  7. Alternativas tecnológicas que permitan la elaboración de productos conformados ricos en ácidos grasos poli-insaturados, a partir de una especie marina grasa sub-explotada (SARACA, Brevoortia aurea)

    OpenAIRE

    Pennisi Forell, Susana Carolina

    2013-01-01

    El presente trabajo de Tesis pretende realizar un aporte a la industria pesquera, en el desarrollo y utilización de especies marinas sin valor comercial. En este caso se busca darle utilidad a la especie conocida como saraca (Brevoortia aurea), la cual se pesca a lo largo del año y principalmente como fauna acompañante durante la zafra de la corvina rubia (Micropogonias furnieri); al no tener valor en el mercado se la descarta. Los volúmenes capturados son considerables por lo que se decidió ...

  8. Evaluación de la bioactividad y papel ecológico de los extractos orgánicos crudos de las esponjas marinas Cribrochalina vasculum y Biemna cribaria

    Directory of Open Access Journals (Sweden)

    Arias Hernández Jazmín

    2005-07-01

    Full Text Available La necesidad por obtener una utilidad tangible de los organismos marinos, ha generado un creciente interés por realizar estudios que permitan entender y por lo tanto aprovechar, sus características físicas, biológicas, químicas y ecológicas. La ecología química permite aproximarse a esto,
    por lo tanto, los estudios desarrollados en esta área encierran gran importancia. En el presente estudio se evaluó la actividad de los extractos orgánicos crudos de las esponjas marinas Cribrochalina vasculum y Biemna cribaria, en ensayos de antiepibiosis y disuasión de la alimentación de peces arrecifales generalistas. Estas dos especies mostraron inhibición positiva de la abundancia total, de organismos solitarios, de poliquetos e hidroides; B. cribaria inhibió adicionalmente la abundancia de cianofíceas, cobertura total y cobertura del alga parda. Así mismo, las píldoras preparadas con extracto de C. vasculum no disuadieron a los peces generalistas Stegastes partitus de alimentarse (97,5% de consumo, pero si mostraron rechazo por las píldoras con extracto de B. cribaria (37,5% de consumo. Lo que parece indicar que la esponja B. cribaria está mejor defendida químicamente contra la epibiosis y la depredación, mientras que C. vasculum parece tener un efecto atrayente sobre los peces, por lo que no mostró efectividad en este ensayo. Se propone a B. cribaria como una esponja con posibilidades en la industria de pinturas antiepibióticas, dada su mayor efectividad
    inhibitoria de epibiontes y su baja tasa de lixiviación. Ya que este es un primer acercamiento a la bioactividad de estas dos esponjas, surge la necesidad por continuar estudiando los aspectos ecológicos y químicos de los metabolitos que ellas producen.

  9. Extracellular proteases from the Antarctic marine Pseudoalteromonas sp. P96-47 strain Proteasas extracelulares de la cepa marina antártica Pseudoalteromonas sp. P96-47

    Directory of Open Access Journals (Sweden)

    S.C. Vázquez

    2008-03-01

    Full Text Available The extracellular protease-production capacity of 33 bacterial isolates taken from marine biotopes in King George Island, Antarctica, was evaluated in liquid cultures. The P96-47 isolate was selected due to its high production capacity and was identified as Pseudoalteromonas sp. The optimal growth temperature was 20 °C and the optimal for protease production was 15 °C. Proteases were purified from culture supernatants, developing a multiple-band profile in zymograms. They were classified as neutral metalloproteases and worked optimally at 45 °C with an Eact of 47 kJ/ mol. Their stability was higher at neutral pH, retaining more than 80% of activity at pH 6-10 after 3 h incubation at 4 °C. After 90 min incubation at 40 and 50 °C, the percentages of residual activities were 78% and 44%. These results contribute to the basic knowledge of Antarctic marine proteases and also help evaluate the probable industrial applications of P96-47 proteases.La capacidad productora de proteasas extracelulares de 33 aislamientos bacterianos tomados de biotopos marinos en la Isla Rey Jorge, Antártida, fue evaluada en cultivo líquido. El aislamiento P96-47 fue seleccionado debido a su alta capacidad productora y fue identificado como Pseudoalteromonas sp. La temperatura óptima de crecimiento fue de 20 °C y la de producción de 15 °C. Las proteasas fueron purificadas a partir del sobrenadante de cultivo, y en los zimogramas desarrollaron un perfil de múltiples bandas. Estas proteasas fueron clasificadas como metaloproteasas neutras y se observó que trabajan óptimamente a 45 °C, con una Eact de 47 kJ/ mol. Su estabilidad fue superior a pH neutro y retuvieron más del 80% de su actividad a pH 6-10 después de 3 h de incubación a 4 °C. Luego de 90 min de incubación a 40 y 50 °C, las actividades residuales fueron 78% y 44%, respectivamente. Los resultados que se presentan en este trabajo contribuyen al conocimiento básico de las proteasas marinas ant

  10. Molecular Basis of Enhanced Activity in Factor VIIa-Trypsin Variants Conveys Insights into Tissue Factor-mediated Allosteric Regulation of Factor VIIa Activity

    DEFF Research Database (Denmark)

    Sorensen, Anders B.; Madsen, Jesper Jonasson; Svensson, L. Anders

    2016-01-01

    -ray crystallography, we show that the introduced 170 loop from trypsin directly interacts with the FVIIa active site, stabilizing segment 215-217 and activation loop 3, leading to enhanced activity. Molecular dynamics simulations and novel fluorescence quenching studies support that segment 215-217 conformation...... is pivotal to the enhanced activity of the FVIIa variants. We speculate that the allosteric regulation of FVIIa activity by TF binding follows a similar path in conjunction with protease domain N terminus insertion, suggesting a more complete molecular basis of TF-mediated allosteric enhancement of FVIIa...

  11. Sedamoodi 2005 : moda ot shkolnikov / Marina Poltavtseva

    Index Scriptorium Estoniae

    Poltavtseva, Marina

    2005-01-01

    Tallinna koolide õpilaste moekonkursist "Sedamoodi" räägib konkursi organisaator Tallinna Kanutiaia Noortemaja direktor Mari Velleste. Sedamoodi 2005 eri vanusegruppide võitjad. Rändauhinnaks on Tauno Kangro skulptuur

  12. Tshto slutshilos v pervom otseke / Marina Tokareva

    Index Scriptorium Estoniae

    Tokareva, Marina

    2001-01-01

    Teadusliku uurimislaeva "Akademik Mstislav Keldõsh" ekspeditsioon tuvastas juba kuu pärast tuumaallveelaeva "Kursk" hukku katastroofi põhjuse, Venemaa võimud räägivad aga seni kolmestversioonist

  13. Konets stoletija / Mati Unt ; per.: Marina Tervonen

    Index Scriptorium Estoniae

    Unt, Mati, 1944-2005

    1999-01-01

    Eesti keeles ilm.: Looming, 1998, nr. 5, pealk.: "Sajandilõpud". Essees refereeritakse Hillel Schwartzi teost "Zeitenwende - Weltende : Visionen beim Wechsel der Jahnhunderte von 990-1999. Braunschweig, 1992

  14. Port: arstid kasutavad narkootikume / Marina Lohk

    Index Scriptorium Estoniae

    Lohk, Marina

    2015-01-01

    Tallinna ülikooli terviseteaduste ja spordi instituudi direktor ja spordibioloog Kristjan Port mainis ETV saates "Vabariigi kodanikud", et töövõime taastamiseks kasutatakse sageli narkootilisi aineid ning seda teevad ka arstid. Kristjan Porti selgitused

  15. Russkije idut! / Ken Livingstone ; interv. Marina Zubakova

    Index Scriptorium Estoniae

    Livingstone, Ken

    2005-01-01

    Londoni linnapea Ken Livingstone venelaste suurenevast huvist Londoni kinnisvara vastu, Londonis toimuvast Vene Majandusfoorumist ja ootustest foorumi suhtes, linnaeelarve prioriteetidest, elamuehitusest, ühistranspordi kasutamise suurenemisest linnas, Londoni kandideerimisest 2012. aasta olümpiamängude korraldajaks. Kommenteerib Nastja Reznitshenko

  16. A Guide for Marina and Harbor Managers

    Science.gov (United States)

    1991-03-01

    and provided feedback to the questionaire . Appendix C shows the names and addresses of the facilities that responded. Three out of these eight...Title 14 5L, California Harbors and Navigation Code 81, Recreational Boat and Sport Fishing Enhancement 321, Act of 1984 Private Planning and...submission of information and 6 limitations. 32L. Recreational Boating and Sport Fishing Enhancement Act of 1984, (Wallop-Breaux Act) Gives an apportionment to

  17. Biotecnología marina y acuicultura

    Directory of Open Access Journals (Sweden)

    Figueras, Antonio

    2014-08-01

    Full Text Available As a result of overfishing and the escalating cost of fishing, fisheries are unable to provide all the marine products that the world market demands. This shortfall is being met by aquaculture. After Japan and Norway, Spain is one of the world’s leading consumers of fish and seafood. To reduce the losses from disease, increase growth and reproduction rates and juveniles survival... Biotechnology can help achieve these goals. However, there is more than food production in the use of Biotechnology in the marine world. Generation of biodiesel, medicines or new materials have their roots on the application of biotechnology to the marine world.Debido a la situación de los caladeros y al encarecimiento del proceso extractivo, el consumo de pescado y marisco se apoya cada vez más en la acuicultura. España es el tercer país consumidor de pescado del mundo, por detrás tan solo de Japón y Noruega. La biotecnología puede acelerar la consecución de estos objetivos. Sin embargo, no todo es producción de alimento; gracias a la biotecnología asociada al mundo acuático podemos generar combustible, energía, medicamentos y muchas otras aplicaciones que nos permiten valorar cada vez con más la riqueza del mar y su biodiversidad.

  18. Moda kak instrument integratsii / Marina Andrejeva

    Index Scriptorium Estoniae

    Andrejeva, Marina

    2008-01-01

    Moebutiigi Fashion Palace uute kollektsioonide demonstratsioon Tallinna restoranis Gloria. Näidati mudeleid itaalia kaubamärkide P.A.R.O.S.H. ja Ermano Severino ning prantsuse kaubamärgi YSL sügis-talvistest kollektsioonidest 2008/2009. Ehted olid inglise disainerilt Stephen Websterilt. Värvifotod

  19. Moda vernulas v Tallin / Marina Poltavtseva

    Index Scriptorium Estoniae

    Poltavtseva, Marina

    2005-01-01

    Tallinnas Viru Keskuses toimuvast moeürituste sarjast FIBIT. Keskne sündmus on Kuldnõela nominentide Katrin Kuldma, Tiina Talumehe, Thea Pilveti ja Evelin Lille kollektsioonide esitamine. Lisas "Primetshai marku!" juttu moekunstnik Oksana Tanditi uuest kollektsioonist, mida näidatakse ka Viru keskuses

  20. Opening Doors for Marina and Carina

    Science.gov (United States)

    Ritchey, Margaret

    2011-01-01

    This article describes the process of becoming a more reflective practitioner in the delivery of pediatric physical therapy through attention to 3 challenges: the therapist's resistance to addressing infant-parent mental health issues, the parents' resistance to acknowledging their infants' delays or disabilities, and the therapist's realization…