WorldWideScience

Sample records for videotape cassettes portraying

  1. Scars. [Videotape].

    Science.gov (United States)

    2002

    This 40-minute videotape tackles the issue of childhood bullying and unwanted teasing and torment. This videotape features real school children handling dramatic roles, and "doing the right thing" (aka "positive modeling.") The film is divided into two distinct parts: first act themes include bullying, girl bullies, children without one or both…

  2. The Carrot Highway [Videotape].

    Science.gov (United States)

    Wyss, Ron

    "The Carrot Highway" is a 40-minute award-winning videotape that takes viewers on a whirlwind tour around the world to tell the story of the carrot. This videotape reveals the carrot in all its glory by cleverly integrating live-action, music, animation, videotape footage, and games. Viewers travel with a troupe of animated carrot characters to…

  3. Videotapes

    CERN Document Server

    Chavanne, A

    1989-01-01

    1666 : impact de la creation de l'Academie des Sciences par Colbert, trente ans apres le proces de Galile, et au moment des disparitions de Pascal, Descartes et Fermat. Elle dirigee par le hollandais Huyggens jusqu'a sa fuite de France au moment de la revocation de l'Edit de Nantes. - 1750 : l'Encyclopedie (ou "Dictionnaire raisonne des Sciences, des Arts et des Metiers") de Diderot et d'Alembert, soutenus par Malherbes, Buffon, Condorcet et Rousseau. - 1789 : Revolution francaise. - 8 aout 1793 : l'Assemblee, par une declaration de Marat, dissout l'Academie des Sciences. Celle-ci continue cependant ses travaux pour les poids et mesures jusqu'en 1795. - la Terreur : la condamnation a mort, pas au nom d'une "Revolution qui n'a pas besoin de savants", de trois grands hommes de science : Lavoisier, Bailly et Condorcet. - 1793-1794 : Au printemps 93, le Comite de Salut Publique s'inquiete du demi-million de soldats etrangers de toutes les pays frontaliers qui essaient de penetrer en France pour occuper le pays. C...

  4. Evaluation of Videotaped and Live Theatre Auditions.

    Science.gov (United States)

    Ward, William C.

    Theatre auditions by 24 semifinalists in the 1980 Scholars in the Arts program were evaluated under two conditions. Four judges ranked the live auditions, while five evaluated videotapes of the same performance of the high school seniors. The auditions were videotaped in black and white. A single camera was used, fixed at an intermediate distance…

  5. Training Parents with Videotapes: Recognizing Limitations

    Science.gov (United States)

    Foster, Brandon W.; Roberts, Mark W.

    2007-01-01

    Among the many methods of teaching skills to parents of disruptive children, videotape modeling of specific parent-child interaction sequences has been particularly effective. Given the likelihood of timeout resistance in defiant children, the authors tested the effectiveness of videotape parent training with a sample of clinic referred,…

  6. Mad scientists, compassionate healers, and greedy egotists: the portrayal of physicians in the movies.

    Science.gov (United States)

    Flores, Glenn

    2002-01-01

    Cinematic depictions of physicians potentially can affect public expectations and the patient-physician relationship, but little attention has been devoted to portrayals of physicians in movies. The objective of the study was the analysis of cinematic depictions of physicians to determine common demographic attributes of movie physicians, major themes, and whether portrayals have changed over time. All movies released on videotape with physicians as main characters and readily available to the public were viewed in their entirety. Data were collected on physician characteristics, diagnoses, and medical accuracy, and dialogue concerning physicians was transcribed. The results showed that in the 131 films, movie physicians were significantly more likely to be male (p film physicians has declined steadily in recent decades. Movie physicians are most commonly surgeons (33%), psychiatrists (26%), and family practitioners (18%). Physicians were portrayed negatively in 44% of movies, and since the 1960s positive portrayals declined while negative portrayals increased. Physicians frequently are depicted as greedy, egotistical, uncaring, and unethical, especially in recent films. Medical inaccuracies occurred in 27% of films. Compassion and idealism were common in early physician movies but are increasingly scarce in recent decades. A recurrent theme is the "mad scientist," the physician-researcher that values research more than patients' welfare. Portrayals of physicians as egotistical and materialistic have increased, whereas sexism and racism have waned. Movies from the past two decades have explored critical issues surrounding medical ethics and managed care. We conclude that negative cinematic portrayals of physicians are on the rise, which may adversely affect patient expectations and the patient-physician relationship. Nevertheless, films about physicians can serve as useful gauges of public opinion about the medical profession, as tools for medical education, and as

  7. Portrayal of Immigrants in Newsmagazines

    Directory of Open Access Journals (Sweden)

    Goran Goldberger

    2004-03-01

    Full Text Available This article analyzes how United States newsmagazines represented immigrants in the aftermath of September 11th terrorist attacks. Methodologically, the paper uses the frame analysis from a social constructivist standpoint, identifying the four functions of frame, as defined by Entman. Three months prior to the attacks, newsmagazines framed immigrants as “needed” and, in most cases, they portrayed them positively. In the period after the attacks, the frame shifted and newsmagazines started representing immigrants as “feared”, potential harborers of terrorists, and so on. Before the attacks, illegal immigrants were represented as the greatest immigration problem. After the attacks, the attention of newsmagazines shifted to legal immigrants with terrorist intentions. The results suggest that the issue of immigrants and immigration policy in the media collided with the threat of terrorism as a foreign policy issue. Thus, it became a security issue that influenced the representation of immigrants. In newsmagazines’ portrayal of immigrants, political features became more prominent than economic ones.

  8. Space Science in Action: Earth's Atmosphere [Videotape].

    Science.gov (United States)

    1999

    In this videotape recording, students learn about the layers of the atmosphere and why each is important to the survival of life on the planet. Students discover why the atmosphere is responsible for weather and see how special aircraft actually fly into hurricanes. Students build their own working barometer in a hands-on activity. Contents…

  9. Space Science in Action: Stars [Videotape].

    Science.gov (United States)

    1999

    This videotape recording shows students the many ways scientists look at the stars and how they can use what they see to answer questions such as What are stars made of?, How far away are they?, and How old are the stars? Students learn about the life span of stars and the various stages they pass through from protostar to main sequence star to…

  10. Space Science in Action: Space Exploration [Videotape].

    Science.gov (United States)

    1999

    In this videotape recording, students learn about the human quest to discover what is out in space. Students see the challenges and benefits of space exploration including the development of rocket science, a look back at the space race, and a history of manned space travel. A special section on the Saturn V rocket gives students insight into the…

  11. Videotaping the Lifespan of a Soap Bubble.

    Science.gov (United States)

    Ramme, Goran

    1995-01-01

    Describes how the use of a videotape to record the history of a soap bubble allows a study of many interesting events in considerable detail including interference fringes, convection and turbulence patterns on the surface, formation of black film, and the ultimate explosion of the bubble. (JRH)

  12. Space Science in Action: Astronomy [Videotape].

    Science.gov (United States)

    1999

    This videotape recording teaches students about constellations, star movement, and how scientists have studied celestial bodies throughout history from Ptolemy to Copernicus to the work of the Hubble Space Telescope. An interview with Kathy Thornton, one of the astronauts who repaired the Hubble while in orbit, is featured. A hands-on activity…

  13. Adding Feminist Therapy to Videotape Demonstrations.

    Science.gov (United States)

    Konrad, Jennifer L.; Yoder, Janice D.

    2000-01-01

    Provides directions for presenting a 32-minute series of four videotape segments that highlights the fundamental features of four approaches to psychotherapy, extending its reach to include a feminist perspective. Describes the approaches and included segments. Reports that students' comments demonstrate that the video sequence provided a helpful…

  14. Assessment of Waco, Texas FLIR videotape

    Science.gov (United States)

    Frankel, Donald S.

    2001-09-01

    The FLIR video recorded by the FBI on 19 April 1993, records the final assault on the Branch Davidian compound in Waco, Texas, and the fire in which some 80 members of the sect died. Attention has focused on a number of flashes recorded on the videotape. The author has examined the 1993 videotape and the recorded videotapes of the re-enactment conducted at Fort Hood, Texas on 19 March 2000. The following conclusions have been reached: 1) The flashes seen on the tape cannot be weapons muzzle flash. Their duration is far too long and their spatial extent is far too great. They are almost certainly the result of solar energy or heat energy form nearby vehicles reflected toward the FLIR by debris or puddles. 2) The FLIR video technology has a very low probability of detecting small arms muzzle flash. 3) As a consequence of 2) above, the absence of muzzle flash detection on the FLIR tape does not prove that no weapons were actually fired during the final assault. Indeed, there is ample evidence (not presented here) that the Davidians fired at the federal agents, but none of their muzzle flashes are detectable on the videotape.

  15. Copyright Handbook for Videotapes and Microcomputer Software.

    Science.gov (United States)

    Ensign, David; And Others

    Developed to help library media personnel, administrators, and educators understand and work with the copyright law as it applies to videotape and microcomputer software, this handbook provides: (1) an overview of copyright, including rights granted to copyright holders and libraries and court interpretation of the copyright law; (2) suggestions…

  16. Comparing Streaming Video and Videotapes: Can Streaming Video Convey Affective Meaning as Well as Videotape?

    Science.gov (United States)

    Cofield, Jay L.

    This study investigated whether or not low-bandwidth streaming video could be useful for affective purposes. A group of 30 students in a cinema course at a public, liberal arts university viewed a 10-minute dramatic video scene by either videotape or low-bandwidth streaming video. They also took a survey to determine their affective responses and…

  17. Waco investigation: analysis of FLIR videotapes

    Science.gov (United States)

    Klasen, Lena M.

    2001-09-01

    This paper presents some of the image processing techniques that were applied to seek an answer to the question whether agents of the Federal Bureau of Investigation (FBI) directed gunfired against the Branch Davidian complex in the tragic event that took place in Waco, Texas, U.S., 1993. The task for this investigation was to provide a scientific opinion that clarified the cause of the questioned events, or flashes, that can be seen on one of the surveillance videotapes. These flashes were by several experts, concluded to be evidence of gunfire. However, there were many reasons to question the correctness of that conclusion, such as the fact that some of the flashes appeared on a regular basis. The main hypothesis for this work was that the flashes instead were caused by specular solar reflections. The technical approach for this work was to analyze and compare the flashes appearance. By reconstructing the spatial and temporal position of the sensor, the complex and the sun, the geometrical properties was compared to the theoretical appearance of specular solar reflections. The result showed that the flashes seen on the FLIR videotape, were caused by solar or heat reflections from single or multiple objects. Consequently, they could not form evidence of gunfire. Further, the result highlights the importance of considering the characteristics of the imaging system within investigations that utilizes images as information source. This is due to the need of separating real data from other phenomena (such as solar reflections), distortions and artifacts in a correct manner.

  18. Finger-Actuated, Self-Contained Immunoassay Cassettes

    OpenAIRE

    Qiu, Xianbo; Thompson, Jason A.; Chen, Zongyuan; Liu, Changchun; Chen, Dafeng; Ramprasad, Sudhir; Mauk, Michael G; Ongagna, Serge; Barber, Cheryl; Abrams, William R.; Malamud, Daniel; Paul L A M Corstjens; Bau, Haim H.

    2009-01-01

    The building blocks for an inexpensive, disposable, luminescence-based microfluidic immunoassay cassette are described, and their integration in a point-of-care diagnostic system is demonstrated. Fluid motion in the cassette is driven by depressing finger-actuated pouches. All reagents needed for the immunoassay can be stored in the cassette in liquid form. Prior to use, the cassette consists of two separate parts. A top storage component contains pouches, sealed storage chambers, a metering ...

  19. All about Bugs. Animal Life for Children. [Videotape].

    Science.gov (United States)

    2000

    Bugs fascinate children, and each kind of bug plays a special role in the circle of life. Some bugs pollinate plants, while others help to decompose plant and animal waste. In this videotape, students learn about the similar characteristics that all bugs share and compare them to their close cousins, the arachnids. This videotape correlates to the…

  20. Critical Time: Earthquake Response Planning and Schools. [Videotape].

    Science.gov (United States)

    Federal Emergency Management Agency, Washington, DC.

    A videotape that describes what earthquakes are, and examines the disaster planning schools can develop during the first few minutes following an earthquake to assure students and staff survive. The kinds of destruction likely to happen during a damaging earthquake are highlighted. The videotape stresses the need for children and staff to know…

  1. All about Birds. Animal Life for Children. [Videotape].

    Science.gov (United States)

    2000

    Winged, feathered friends helped to inspire the airplane and have always interested human bird watchers. In this videotape, children learn about the main characteristics of birds and look at their similar needs. Students find out about the process of egg laying and hatching in some of the most common birds. This videotape correlates to the…

  2. All about Mammals. Animal Life for Children. [Videotape].

    Science.gov (United States)

    2000

    In this videotape, students learn more about the characteristics of common warm-blooded mammals and what makes them different from other animals. Children also find out how humans are more advanced in structure than other mammals, but how they still share the same basic traits. This videotape correlates to the following National Science Education…

  3. Fuel cell cassette with compliant seal

    Science.gov (United States)

    Karl, Haltiner, Jr. J.; Anthony, Derose J.; Klotzbach, Darasack C.; Schneider, Jonathan R.

    2017-11-07

    A fuel cell cassette for forming a fuel cell stack along a fuel cell axis includes a cell retainer, a plate positioned axially to the cell retainer and defining a space axially with the cell retainer, and a fuel cell having an anode layer and a cathode layer separated by an electrolyte layer. The outer perimeter of the fuel cell is positioned in the space between the plate and the cell retainer, thereby retaining the fuel cell and defining a cavity between the cell retainer, the fuel cell, and the plate. The fuel cell cassette also includes a seal disposed within the cavity for sealing the edge of the fuel cell. The seal is compliant at operational temperatures of the fuel cell, thereby allowing lateral expansion and contraction of the fuel cell within the cavity while maintaining sealing at the edge of the fuel cell.

  4. Problems Portraying Migrants in Applied Linguistics Research

    Science.gov (United States)

    Block, David

    2010-01-01

    This paper is a very personal attempt to explore the problematics of portraying migrants in Applied Linguistics research. I begin with a discussion of identity, in particular what we might mean when we use the term, and from there I go on to explore its fundamental imprecision through an analysis of a census question about ethnicity. I then…

  5. Portrayal of gay characters in Bollywood cinema.

    Science.gov (United States)

    Bhugra, Dinesh; Kalra, Gurvinder; Ventriglio, Antonio

    2015-01-01

    Hindi cinema production from India is the largest in the world. Cinema reflects the society which it portrays, and in turn influences social attitudes. Hindi cinema, often known as Bollywood, demonstrates deep roots in society. In the last decade homosexual behaviour was legalized by the Delhi High Court but subsequently deemed illegal by the Supreme Court. These changes reflect changes in social attitudes. In this paper we highlight social attitudes to homosexuality - both male and female - in the context of portrayal in Hindi cinema. Historically, cultural and social values and the attitudes towards sexuality in India have been sex positive, but over the past 200 years under the British colonial rule they became very negative and indeed punitive towards homosexuality and homosexual men and women in line with prevalent Victorian attitudes to sex and sexual activity. Various descriptions and sexual identities have been identified and described in the literature. In this paper we describe a small selection of films from Hindi cinema which depict homosexuality. These portrayals have been largely negative. We describe the possible reasons for this and the potential impact these portrayals may have on audiences.

  6. Still Photography: Can It Provide Program Portrayal?

    Science.gov (United States)

    Templin, Patricia Scheyer

    Still photography can help portray educational programs for description and evaluation. There may, however, be problems with this method. Photographic images idealize the subjects; the framing is arbitrary, lends importance and veracity at the same time it levels an event to a very thin slice of reality. The evaluator-photographer may have…

  7. Disinfection Effect of Film Cassettes by Ultraviolet Irradiation

    Energy Technology Data Exchange (ETDEWEB)

    Kweon, Dae Cheol; Park, Peom [Ajou Univ., Suwon (Korea, Republic of)

    2001-12-15

    A bacteria infection on film cassette contact surface was examined at the diagnostic radiology department. Studies have demonstrated a bactericidal effect of ultraviolet irradiation, and to assess the contamination level on film cassette contact surface as a predictor of patient prevent from nosocomial infection. The study showed that the laboratory result was identified non-pathologic and pathologic bacterial in the five different cassette size of the contact surface. Film cassettes were exposed to ultraviolet light for 1, 2 and 3 minutes. Ultraviolet light disinfection practices suitable for bacteria. The study concludes that presence of a bacterial infection will prevent a using antiseptic technique on film cassette contact surface. In conclusion, ultraviolet irradiate on film cassette over the surface more than 2 minutes. Ultraviolet dose of 1565 {mu}W {center_dot} s/cm{sup 2}Win in 30 second relative to ultraviolet dose in time.

  8. Portrayal of Women's Images in Television Dramas: A Malaysian Case

    National Research Council Canada - National Science Library

    Faridah Ibrahim; Norzita Yunus; Dil Froz Jan Sayed Halem Shah; Munirah Ilias; Amli Hazlin Ahmad Mokhtar

    2017-01-01

    ... is their centrality in the media, through media positions held or through media images portrayed. What this paper has endeavoured to show is the portrayal of women in the media particularly, television dramas...

  9. Usefulness of Videotape Instruction in an Academic Department of Neurology.

    Science.gov (United States)

    Kaufman, David M.; Kaufman, Rita G.

    1983-01-01

    Videotape instruction produced better performance in identification in only certain areas in a neurology clerkship: neuropsychologic phenomena, disorders with subtle or unique movements, and seizures. The choice and cost of equipment and some professional assurances are discussed. (Author/MLW)

  10. A study on contamination and disinfection of film cassette

    Energy Technology Data Exchange (ETDEWEB)

    Kweon, Dae Cheol; Chung, Kyung Mo [Seoul National University Hospital, Seoul (Korea, Republic of); Choi, Ji Won [University of Sydney, Sydney (Australia)

    2000-04-15

    In July 2000, a bacteria infection on film cassette contact surface was examined at the diagnostic radiology department of the S. hospital. The objective of this study was to assess the contamination level on film cassette contact surface as a predictor of patient to prevent from nosocomial infection. The study showed that the laboratory result was identified non-pathologic bacterial in the four different cassette size of the contact surface. The study concludes that presence of a bacterial infection will prevent a using antiseptic technique on film cassette contact surface. Also the education of nosocomial infection for radiographer will be required.

  11. A Cassette Based System for Hydrogen Storage and Delivery

    Energy Technology Data Exchange (ETDEWEB)

    Britton Wayne E.

    2006-11-29

    A hydrogen storage system is described and evaluated. This is based upon a cassette, that is a container for managing hydrogen storage materials. The container is designed to be safe, modular, adaptable to different chemistries, inexpensive, and transportable. A second module receives the cassette and provides the necessary infrastructure to deliver hydrogen from the cassette according to enduser requirements. The modular concept has a number of advantages over approaches that are all in one stand alone systems. The advantages of a cassette based system are discussed, along with results from model and laboratory testing.

  12. Finger-Actuated, Self-Contained Immunoassay Cassettes

    Science.gov (United States)

    Qiu, Xianbo; Thompson, Jason A.; Chen, Zongyuan; Liu, Changchun; Chen, Dafeng; Ramprasad, Sudhir; Mauk, Michael G.; Ongagna, Serge; Barber, Cheryl; Abrams, William R.; Malamud, Daniel; Corstjens, Paul L.A.M.; Bau, Haim H.

    2010-01-01

    The building blocks for an inexpensive, disposable, luminescence-based microfluidic immunoassay cassette are described, and their integration in a point-of-care diagnostic system is demonstrated. Fluid motion in the cassette is driven by depressing finger-actuated pouches. All reagents needed for the immunoassay can be stored in the cassette in liquid form. Prior to use, the cassette consists of two separate parts. A top storage component contains pouches, sealed storage chambers, a metering chamber, and needle seats. The bottom processing component contains connection needles, a mixing chamber, and a detection chamber with immobilized proteins. Subsequent to sample introduction, the storage and processing components are mated. The needles form hydraulic connections between the two parts and, in some cases, close valves. The pouches are then actuated sequentially to induce flow of various reagents and facilitate process operations. The cassette is compatible with different detection modalities. Both a cassette with immunochromatographic-based detection and a cassette with microbead-based detection were constructed and evaluated. The immunochromatographic cassette was used to detect antibodies to HIV in saliva samples. The bead-based cassette was used to detect the proinflammatory chemokine IL-8. The experimental data demonstrates good repeatability and reasonable sensitivity. PMID:19597994

  13. The Portrayal of Women in Newspapers: A Meta-Analysis.

    Science.gov (United States)

    Greenwald, Marilyn S.

    To investigate the role and portrayal of women in newspapers and to determine trends emerging from this portrayal, a study examined 15 published quantitative research studies (content analyses) on the portrayal of women in newspapers, dating from 1973 until 1988. Studies were gathered primarily from "Communication Abstracts," the…

  14. Crealivity: using creativity to portray reality

    OpenAIRE

    Palmer, Clive Alan; Creswell, Anna; Loveday, Rachel

    2015-01-01

    WARNING: The social topics dealt with in this paper are disturbing, being uncomfortably close to the surface of our everyday society; child abduction, paedophile rings, the child sex trade and child prostitution. These topics are portrayed through two creative artworks; a short story and a pictorial image. In combination they illustrate with shocking clarity how easily abuse can start and where it can end. The endeavour is to impart a message about the vulnerability of children through access...

  15. Portrayed emotions in the movie "Forrest Gump"

    OpenAIRE

    Annika Labs; Theresa Reich; Helene Schulenburg; Manuel Boennen; Gehrke Mareike; Madleen Golz; Benita Hartigs; Nico Hoffmann; Sebastian Keil; Malú Perlow; Anne Katrin Peukmann; Lea Noell Rabe; Franca-Rosa von Sobbe; Michael Hanke

    2015-01-01

    Here we present a dataset with a description of portrayed emotions in the movie ”Forrest Gump”. A total of 12 observers independently annotated emotional episodes regarding their temporal location and duration. The nature of an emotion was characterized with basic attributes, such as arousal and valence, as well as explicit emotion category labels. In addition, annotations include a record of the perceptual evidence for the presence of an emotion. Two variants of the movie were annotated sepa...

  16. Recombination between the dfrA12-orfF-aadA2 Cassette Array and an aadA1 Gene Cassette Creates a Hybrid Cassette, aadA8b

    Science.gov (United States)

    Gestal, Alicia M.; Stokes, H. W.; Partridge, Sally R.; Hall, Ruth M.

    2005-01-01

    Homologous recombination between closely related gene cassettes, such as aadA1 and aadA2, which are 89% identical, can create hybrid cassettes and hybrids of existing cassette arrays. A new cassette array, dfrA12-orfF-aadA8b, which was created by such a recombination event occurring within the aadA2 cassette in the dfrA12-orfF-aadA2 array, has been identified. PMID:16251327

  17. Associations between dru Types and SCCmec Cassettes

    DEFF Research Database (Denmark)

    Bartels, Mette D; Boye, Kit; Oliveira, Duarte C

    2013-01-01

    isolates with dru type variants indicating that dru typing is not useful as a first line epidemiological typing tool. However, MRSA isolates cultured from a single patient over a three year period exhibited a single dru type. The finding of dt10a in most SCCmec types suggests that dru and mecA originate......Molecular typing is an important tool in the investigation of methicillin resistant Staphylococcus aureus (MRSA) outbreaks and in following the evolution of MRSA. The staphylococcal cassette chromosome mec (SCCmec) contains a hypervariable region with a variable number of 40 bp repeats named direct...... repeat units (dru). The dru region has been suggested as a supplementary typing method for MRSA and an international nomenclature exists. The purpose of this study was to investigate the diversity and variability of the dru region in a diverse collection of MRSA. We studied 302 MRSA isolates harbouring...

  18. Folk musical dramas portray population issues. Gurgaon.

    Science.gov (United States)

    1994-01-01

    The State Population Education Cell of the State Council of Educational Research and Training (SCERT) is now testing the use of folk musical dramas in promoting population messages. Being the language of the hearts, it is expected that about 50,000 students, teachers and members of the community will be oriented about the problems arising from rapid population growth through folk musical dramas. These musical dramas are innovative vehicles adopted under the Village Adoption and Population Education Laboratory Schools by the SCERT. It follows three phases. The first phase required poet teachers to develop three folk musical dramas on the themes of status of women and gender disparity, importance of small family and population growth and its impact on environment. The second phase saw the staging of these folk musical dramas in two adopted schools. The presentations were video taped and recorded into audio-video cassettes. For the last phase, another workshop will be organized to convene teacher poets to develop more folk musical dramas on the themes of adolescent problem, aging problem, mother and child care, delayed marriage, status of women and population growth and environment. The video cassettes produced previously will be used in the training of teachers, students and heads of schools on the development of folk musical dramas. After editing these dramas, they will be published in the form of booklets to promote wider dissemination to schools, which can stage these ready-made folk musical dramas during school celebrations and during the Population Education Week. full text

  19. Portrayed emotions in the movie "Forrest Gump".

    Science.gov (United States)

    Labs, Annika; Reich, Theresa; Schulenburg, Helene; Boennen, Manuel; Mareike, Gehrke; Golz, Madleen; Hartigs, Benita; Hoffmann, Nico; Keil, Sebastian; Perlow, Malú; Peukmann, Anne Katrin; Rabe, Lea Noell; von Sobbe, Franca-Rosa; Hanke, Michael

    2015-01-01

    Here we present a dataset with a description of portrayed emotions in the movie "Forrest Gump". A total of 12 observers independently annotated emotional episodes regarding their temporal location and duration. The nature of an emotion was characterized with basic attributes, such as arousal and valence, as well as explicit emotion category labels. In addition, annotations include a record of the perceptual evidence for the presence of an emotion. Two variants of the movie were annotated separately: 1) an audio-movie version of Forrest Gump that has been used as a stimulus for the acquisition of a large public functional brain imaging dataset, and 2) the original audio-visual movie. We present reliability and consistency estimates that suggest that both stimuli can be used to study visual and auditory emotion cue processing in real-life like situations. Raw annotations from all observers are publicly released in full in order to maximize their utility for a wide range of applications and possible future extensions. In addition, aggregate time series of inter-observer agreement with respect to particular attributes of portrayed emotions are provided to facilitate adoption of these data.

  20. Breaststroke learning through the use of videotape feedback

    Directory of Open Access Journals (Sweden)

    Marcela de Castro Ferracioli

    2013-03-01

    Full Text Available DOI: http://dx.doi.org/10.5007/1980-0037.2013v15n2p204 People from all age groups and social backgrounds have always sought to learn swimming. However, the swimming learning process is usually considered repetitive and tiring, requiring the teacher to use methods that motivate students to join the practice without ignoring the need for improvement in their performance. This study assessed motivation during a breaststroke learning process in students who received videotape feedback, verbal feedback, and who did not receive any feedback during practice. Thirty seven swimming inexperienced students were divided into three groups: Video (n=13, which received videotape feedback; Verbal (n=15, which received verbal feedback; and Control (n=9, which did not receive any feedback during experimental phases (pre-test, acquisition (5 days, post-test and retention. Participants completed a questionnaire based on Likert scale for motivation assessment. Scores were given to their performance by a swimming teacher to assess breaststroke learning during each experimental phase. Results of motivation assessment showed that students who received feedback (videotape or verbal felt more motivated during practice than those who did not receive any feedback. Regarding the breaststroke learning, all participants improved their performance along experimental phases, but, during the retention one, Verbal group’s performance was considered superior to the Control group’s performance. This study concluded that the use of videotape and verbal feedback has motivational results on breaststroke learning, and that it is effective in the learning process.

  1. Space Science for Children: All about Stars [Videotape].

    Science.gov (United States)

    1999

    This 23-minute videotape features information, for children in grades K-4, about stars including their composition, brightness, and location. How stars are formed, why they seem to move across our sky, and how humans have used their patterns to form the constellations are discussed. A hands-on activity related to the reflecting telescope is also…

  2. All about Amphibians. Animal Life for Children. [Videotape].

    Science.gov (United States)

    2000

    This videotape teaches children about their favorite amphibious creatures, as well as amphibians' nearest cousins--toads, newts, and salamanders. Young students discover how these amazing creatures can live both in and out of water, learn about the amphibious life cycle, and compare the differences between amphibians and reptiles. This videotape…

  3. Me & Isaac Newton. [Videotape and] KIDSNET Guide for Educators.

    Science.gov (United States)

    2001

    This videotape records the stories of 7 scientists, 3 women and 4 men who range in age from 33 to 81. Beginning with their earliest scientific questions and including their most personal ponderings, the scientists reveal their histories and professional obligations to affect the world. Director Michael Apted allows the personal adventures of the…

  4. Use of Videotape Feedback with Severely Disturbed Adolescents.

    Science.gov (United States)

    Michel, Jane; Blitstein, Sheldon

    1979-01-01

    Summarizes the design and effects of a group therapy project using videotape feedback with seriously disturbed adolescents. Offers anecdotal evidence that the feedback facilitated the correction of the participants' distorted body images, low self-esteem, lack of capacity for self-observation, and poor peer relationships. (SS)

  5. All about Dinosaurs. Animal Life for Children. [Videotape].

    Science.gov (United States)

    2000

    Dinosaurs were the rulers of the land 65 million years ago. In this videotape, children learn more about the different kinds of dinosaurs by viewing vivid illustrations and fossil discoveries. Students compare the dinosaurs to their modern kin--snakes, lizards, and crocodiles. Students also listen to different theories to try to answer the big…

  6. All about Reptiles. Animal Life for Children. [Videotape].

    Science.gov (United States)

    2000

    Dinosaurs may be extinct, but reptiles are distant cousins to the beasts that once walked the earth. From snakes and lizards to iguanas and tuataras, children learn what factors make them different from other animals. In this videotape, students explore the mysterious, often misunderstood, world of reptiles and learn about their characteristics…

  7. All about Animal Behavior & Communication. Animal Life for Children. [Videotape].

    Science.gov (United States)

    2000

    Why do animals do what they do? What is the difference between instinct and learned behavior? How do animals communicate? These questions are answered as children examine animal behaviors that help them find food, protect themselves, and care for their young. This videotape correlates to the following National Science Education Standards for Life…

  8. All about Food Chains. Animal Life for Children. [Videotape].

    Science.gov (United States)

    2000

    Whether animals are herbivores, carnivores, or omnivores, each one is part of an eternal food chain that carries on from one generation to the next. In this videotape, students learn more about terms like "predator,""pre-consumer" and "producer," as well as the cycles of food chains and food webs and how they support…

  9. Observing cassette culture: user interface implications for digital music libraries

    OpenAIRE

    Toal, Jason

    2007-01-01

    Many people keep their collections of music on cassette tape even if they rarely listen to them. Images of these collections can be found online on photo sharing websites. What can we learn from such collections and what might they tell us about designing interfaces for new digital music libraries? The author conducts an online ethnographic study of over two hundred cassette tape collections, and over sixty participants with the aim of guiding future design of music collections. The author pr...

  10. Transcription, Processing, and Function of CRISPR Cassettes in Escherichia coli

    OpenAIRE

    Pougach, Ksenia; Semenova, Ekaterina; Bogdanova, Ekaterina; Datsenko, Kirill A.; Djordjevic, Marko; Wanner, Barry L.; Severinov, Konstantin

    2010-01-01

    CRISPR/Cas, bacterial and archaeal systems of interference with foreign genetic elements such as viruses or plasmids, consist of DNA loci called CRISPR cassettes (a set of variable spacers regularly separated by palindromic repeats) and associated cas genes. When a CRISPR spacer sequence exactly matches a sequence in a viral genome, the cell can become resistant to the virus. The CRISPR/Cas systems function through small RNAs originating from longer CRISPR cassette transcripts. While laborato...

  11. Producing Videotape Programs for Computer Training: An Example with AMA/NET

    OpenAIRE

    Novey, Donald W.

    1990-01-01

    To facilitate user proficiency with AMA/Net, an 80-minute training videotape has been produced. The production was designed to use videotape's advantages, where information and emotion are combined; and to accommodate its chief disadvantage, lack of resolution for fine text, with close-ups and graphics. Content of the videotape was conceived, outlined, demonstrated with simultaneous text capture, edited into script form, narration added, and scripts marked for videotaping and narrating. Video...

  12. Producing Videotape Programs for Computer Training: An Example with AMA/NET

    OpenAIRE

    Novey, Donald W.

    1990-01-01

    To facilitate user proficiency with AMA/Net, an 80-minute training videotape has been produced. The production was designed to use videotape's advantages, where information and emotion are combined; and to accommodate its chief disadvantage, lack of resolution for fine text, with close-ups and graphics. Content of the videotape was conceived, out-lined, demonstrated with simultaneous text capture, edited into script form, narration added, and scripts marked for videotaping and narrating. Vide...

  13. Teaching diagnostic reasoning: using a classroom-as-clinic methodology with videotapes.

    Science.gov (United States)

    Neistadt, M E; Smith, R E

    1997-05-01

    The purpose of this study was to examine the effect of a "classroom-as-clinic" format, using videotaped occupational therapy evaluations, on students' diagnostic reasoning skills. In the classroom-as-clinic format, students write a problem list on the basis of preliminary client information before viewing the videotape. A post-hoc experimental design was used to compare the accuracy of treatment plan problem lists for two groups of senior occupational therapy students--one group viewed two videotapes of client-therapist interactions without a classroom-as-clinic format (n = 82), and one group viewed the same videotapes within the context of a classroom-as-clinic format (n = 45). Both groups viewed the same two videotapes. Videotape 1 was of a client with a brain stem infarct, and Videotape 2 was of a client with traumatic brain injury. Subjects experiencing a classroom-as-clinic format identified significantly more occupational therapy problems for Videotape 1 than those who did not have preevaluation information. There was no significant difference between the two subject groups in the accuracy of their problem lists for Videotape 2. Only subjects in the non-classroom-as-clinic group showed a significant improvement from Videotape 1 to Videotape 2 in occupational therapy problem identification. This study suggests that to be truly effective when used videotapes, the classroom-as-clinic methodology needs to be combined with explicit coaching in problem sensing and problem definition.

  14. The portrayal of women in South African television commercials ...

    African Journals Online (AJOL)

    Much research has been conducted on the portrayals of men and women in advertising. Advertisers are often criticised for portraying stereotypical gender roles, and particularly for depicting women as dependant (such as a homemaker) or decorative (such as a sex object). The purpose of the study on which the article is ...

  15. Women as Home Caregivers: Gender Portrayal in OTC Drug Commercials.

    Science.gov (United States)

    Craig, R. Stephen

    1992-01-01

    Investigated sample of prime time network television advertisements to determine how gender portrayals differ in drug and nondrug commercials. Found that women were significantly more likely than men to appear as characters in drug advertisements than in advertisements for other products and that they were frequently portrayed in these commercials…

  16. Portrayal of Women Characters in Selected Contemporary Yoruba ...

    African Journals Online (AJOL)

    Nekky Umera

    Abstract. Contemporary Yoruba novelists and critics have done tremendous works in portraying female characters in their literary production. For example,. Okediji (1981) in Atoto Arere portrays female characters as mother, wife, sister, prostitute, harsh and malicious, who always keep malice against others. Some other ...

  17. Blacks in Research: How Shall We Be Portrayed?

    Science.gov (United States)

    Fenwick, Leslie T.

    2016-01-01

    A version of this article, "Blacks in Research? How Shall We Be Portrayed?", was delivered by the author as the 2013 W. E. B. Du Bois Distinguished Lecture to the annual conference of the American Educational Research Association (AERA) Blacks in Education Special Interest Group (Black SIG). This article examines the portrayal of Blacks…

  18. Aging and masculinity: portrayals in men's magazines.

    Science.gov (United States)

    Hurd Clarke, Laura; Bennett, Erica V; Liu, Chris

    2014-12-01

    Textual and visual representations of age are instructive as they suggest ideals towards which individuals should strive and influence how we perceive age. The purpose of our study was to investigate textual and visual representations of later life in the advertisements and interest stories of six widely read North American male-oriented magazines (namely, Esquire, GQ, Maxim, Men's Health, Men's Journal, and Zoomer). Through a content analysis and a visual textual analysis, we examined how older men were depicted in the magazine images and accompanying texts. Our findings revealed that older men were largely absent, and when portrayed, were positively depicted as experienced and powerful celebrities or as healthy and happy unknown individuals. The magazine advertisements and interest stories collectively required individuals to engage in consumer culture in order to achieve age and masculinity ideals and stave off the transition from the Third Age to the Fourth Age. We consider our findings in relation to theorizing about ageism, age relations, the Third and Fourth Ages, and idealized aging masculinity. Copyright © 2014 Elsevier Inc. All rights reserved.

  19. Media portrayals of the female condom.

    Science.gov (United States)

    Chatterjee, Karishma; Markham Shaw, Charla

    2012-01-01

    This study examines newspaper and broadcast news depictions of the female condom from 1993 to 2009 in the three major television networks (ABC, CBS, and NBC), and in 8 of 10 top daily newspapers and 3 of 4 national newspapers published in the United States, according to circulation figures as of September 30, 2008. Given the high rates of sexually transmitted infections in the United States and their effect on women, particularly those between 15 and 24 years of age, it is important to examine media sources and the health information that the public is or is not receiving through these sources. This study (a) examines whether the female condom was depicted positively or negatively in broadcast and newspaper coverage, (b) determines the ways in which the female condom was compared with the male condom and with other contraceptives, and (c) identifies the sources used by journalists in female condom portrayals. The authors discuss the implications of the coverage of the female condom by broadcast and print news.

  20. The Cape Times's portrayal of school violence

    Directory of Open Access Journals (Sweden)

    Corene de Wet

    2016-05-01

    Full Text Available This study explores the Cape Times's portrayal of school violence in the Western Cape (WC, South Africa, reporting on findings from a qualitative content analysis of 41 news articles retrieved from the SA Media database. The findings shed light on the victims and their victimisation, the perpetrators, as well as the context of the violence, identifying gangsterism, as well as school administrative and community factors as the reasons for violence in WC schools. It is argued that school violence and gangsterism are inextricably linked to the Cape Flats in particular, and that the interaction of forms of inequality and oppression such as racism, class privilege and gender oppression are structural root causes for school violence in this area of the WC. The study highlights the negative consequences of school violence on teaching and learning and on the economy. It is concluded that even if the Cape Times paints an exaggerated and atypical picture of violence in the gang-riddled parts of the WC, the detrimental effects thereof on the regions cannot be denied. The study therefore recommends a holistic approach to addressing the structural root causes of school violence where it takes place in the WC.

  1. 36 CFR 1280.50 - What will I be allowed to film, photograph, or videotape for news purposes?

    Science.gov (United States)

    2010-07-01

    ... film, photograph, or videotape for news purposes? 1280.50 Section 1280.50 Parks, Forests, and Public... film, photograph, or videotape for news purposes? (a) NARA will permit you to film, photograph, or..., photograph, or videotape if you intend to use the film, photographs, or videotape for commercial, partisan...

  2. [Integrons and resistance gene cassettes: structure and role against antimicrobials].

    Science.gov (United States)

    González, Gerardo; Mella, Sergio; Zemelman, Raúl; Bello, Helia; Domínguez, Mariana

    2004-05-01

    Bacteria have developed sophisticated and successful genetic mechanisms to evade the action of antimicrobials. Bacterial multiresistance has caused serious problems in the treatment of nosocomial infections. Integrons and gene cassettes are considered the main genetic elements in the evolution of plasmids and transposons that actively participate in the mobilization of genes, codifying different bacterial resistance mechanisms. This article reviews the historical and structural aspects of integrons and resistance gene cassettes and the presence of these structures in gram negative bacteria isolated from Chilean hospitals in the last ten years.

  3. Portrayals of Romanian migrants in ethnic media from Italy

    Directory of Open Access Journals (Sweden)

    Roxana Bratu

    2014-12-01

    Full Text Available Based on a qualitative content analysis of a small corpus of newspaper articles, the paper aims to identify the representations of Romanian migrants in the Romanian media from Italy by exploring the portrayals of the main characters and the narratives used to support these portrayals. Arguing that there are important differences between the narrative styles employed for depicting different types of characters, I seek to discuss the implications these differences may have on the conveyed message.

  4. Videotaped recording as a method of participant observation in psychiatric nursing research.

    Science.gov (United States)

    Latvala, E; Vuokila-Oikkonen, P; Janhonen, S

    2000-05-01

    This paper describes videotaped recording as a data collection method when conducting participant observation in a psychiatric nursing study. The videotaped episodes were part of the daily life of psychiatric nursing in a hospital environment. The advantages and limitations of using videotaped recording in nursing research will be discussed. This paper is based on two studies. The data consisted of 21 videotaped episodes of nursing report sessions or interdisciplinary team meetings in the psychiatric clinic of a university hospital. The participants consisted of patients, their significant others, nurses, doctors, social workers and physiotherapists. All videotaped material was transcribed verbatim. An essential advantage of videotaping is that most potentially useful interaction and behaviour can be captured. The advantage in terms of the credibility of videotaping was that the investigator was able to review the same videotaped situations again and again. Videotaped material is rich and provides several possibilities for analysing the data. In these studies data and source triangulation enabled the researchers to reduce personal influence on the results. The investigator must also be aware of the limitations concerning this method. The most essential limitations are mechanical problems and the influence of videotaping on behaviour. Careful ethical considerations are important concerning personal privacy, informed consent and respect for the self-determination of psychiatric patients.

  5. The Real World Spanish Cassette Program. Script Book.

    Science.gov (United States)

    Sternburg, Sheldon G.

    This dual cassette program, accompanied by a script book, is designed to give students listening practice in Spanish, particularly for regional differences of pronunciation and for variety in idiomatic construction. The program may be integrated with texts used in intermediate and advanced levels of instruction. The announcements, jingles, and…

  6. [Integrons and antimicrobial resistance gene cassettes in Shigella flexneri strains ].

    Science.gov (United States)

    Muñoz, Jeannette; Bello, Helia; Domínguez, Mariana; Mella, Sergio; Zemelman, Raúl; González, Gerardo

    2003-07-01

    The resistance of Shigella flexneri to antimicrobial agents can be associated to the presence of integrons that may contain and express antimicrobial resistance gene cassettes. To study antimicrobial resistance and the presence of integrons and antimicrobial gene cassettes in Shigella flexneri strains. In vitro susceptibility to 27 antimicrobials was studied in twenty four Shigella flexneri strains isolated from stools. The presence of integrons class 1, 2 and 3 and antimicrobial resistance gene cassettes was investigated by polymerase chain reaction (PCR) using specific primers for each gene. Most strains were resistant to one of the following antimicrobials: ampicillin, sulphonamide, trimethoprim, tetracycline, streptomycin, sulfamethoxazole-trimethoprim or chloramphenicol. Twenty nine percent were simultaneously resistant to all these antimicrobials. Integrons class 1 and 2 were found in 19 strains (79%). Class 3 integrons were not found. Gene cassettes dfrA1 and ant(3")I were associated to integrons class 2 in most strains (15/20, 75%). Genes cat, tetB and blarTEM were detected in 18/24 (75%), 7/24 (29%) and 4/24 (17%) of the strains, respectively and were not associated to any of the studied integrons. Genes that codify enzymes AAC(6')Ib and APH(3')VI were not detected in any strain. The high frequency of integrons found in the studied strains, could partly explain the increasing antimicrobial resistance of Shigella flexneri strains, isolated in Chile.

  7. Co-assortment in integron-associated gene cassette assemblages in environmental DNA samples

    Directory of Open Access Journals (Sweden)

    Michael Carolyn A

    2010-08-01

    Full Text Available Abstract Background It has been shown that integron-associated gene cassettes exist largely in tandem arrays of variable size, ranging from antibiotic resistance arrays of three to five cassettes up to arrays of more than 100 cassettes associated with the vibrios. Further, the ecology of the integron/gene cassette system has been investigated by showing that very many different cassettes are present in even small environmental samples. In this study, we seek to extend the ecological perspective on the integron/gene cassette system by investigating the way in which this diverse cassette metagenome is apportioned amongst prokaryote lineages in a natural environment. Results We used a combination of PCR-based techniques applied to environmental DNA samples and ecological analytical techniques to establish co-assortment within cassette populations, then establishing the relationship between this co-assortment and genomic structures. We then assessed the distribution of gene cassettes within the environment and found that the majority of gene cassettes existed in large co-assorting groups. Conclusions Our results suggested that the gene cassette diversity of a relatively pristine sampling environment was structured into co-assorting groups, predominantly containing large numbers of cassettes per group. These co-assorting groups consisted of different gene cassettes in stoichiometric relationship. Conservatively, we then attributed co-assorting cassettes to the gene cassette complements of single prokaryote lineages and by implication, to large integron-associated arrays. The prevalence of large arrays in the environment raises new questions about the assembly, maintenance and utility of large cassette arrays in prokaryote populations.

  8. Co-assortment in integron-associated gene cassette assemblages in environmental DNA samples.

    Science.gov (United States)

    Michael, Carolyn A; Andrew, Nigel R

    2010-08-10

    It has been shown that integron-associated gene cassettes exist largely in tandem arrays of variable size, ranging from antibiotic resistance arrays of three to five cassettes up to arrays of more than 100 cassettes associated with the vibrios. Further, the ecology of the integron/gene cassette system has been investigated by showing that very many different cassettes are present in even small environmental samples. In this study, we seek to extend the ecological perspective on the integron/gene cassette system by investigating the way in which this diverse cassette metagenome is apportioned amongst prokaryote lineages in a natural environment. We used a combination of PCR-based techniques applied to environmental DNA samples and ecological analytical techniques to establish co-assortment within cassette populations, then establishing the relationship between this co-assortment and genomic structures. We then assessed the distribution of gene cassettes within the environment and found that the majority of gene cassettes existed in large co-assorting groups. Our results suggested that the gene cassette diversity of a relatively pristine sampling environment was structured into co-assorting groups, predominantly containing large numbers of cassettes per group. These co-assorting groups consisted of different gene cassettes in stoichiometric relationship. Conservatively, we then attributed co-assorting cassettes to the gene cassette complements of single prokaryote lineages and by implication, to large integron-associated arrays. The prevalence of large arrays in the environment raises new questions about the assembly, maintenance and utility of large cassette arrays in prokaryote populations.

  9. Reaching Out: The Role of Audio Cassette Communication in Rural Development. Occasional Paper 19.

    Science.gov (United States)

    Adhikarya, Ronny; Colle, Royal D.

    This report describes the state-of-the-art of audio cassette technology (ACT) and reports findings from field tests, case studies, and pilot projects in several countries which demonstrate the potential of audio cassettes as a medium for communicating with rural people. Specific guidance is also offered on how a project can use cassettes as a…

  10. Hollywood on tobacco: how the entertainment industry understands tobacco portrayal.

    Science.gov (United States)

    Shields, D L; Carol, J; Balbach, E D; McGee, S

    1999-01-01

    To determine how people in the California-based entertainment industry think about the portrayal of tobacco use in movies and on television. Specifically, to explore who decides when to include tobacco in a project; how that decision is made; what issues are considered; what messages are intended; whether and how the issue of second-hand smoke is considered; and what advocacy methods might be useful in influencing future decisions about tobacco portrayal. Qualitative in-depth interviews of entertainment industry personnel,with a semi-structured interview protocol to guide the interview. 54 subjects drawn from a convenience sample of writers, actors, directors, producers, studio executives, and others involved in the film industry. Hollywood is heterogeneous with varying perspectives on rates of tobacco use portrayal; intentionality of the decision to use and the necessity to portray tobacco use; and its degree of acceptance of responsibility for influencing societal smoking. Tobacco depiction may originate with the writer, actor, or director and is included most frequently to elucidate character or portray reality. On-camera smoking is influenced by actors' off-camera tobacco use. The research presented can help advocates better understand the norms and values of those working within the entertainment industry and thereby assist them in creating more effective change strategies.

  11. Graphic Depictions: Portrayals of Mental Illness in Video Games.

    Science.gov (United States)

    Shapiro, Samuel; Rotter, Merrill

    2016-11-01

    Although studies have examined portrayals of mental illness in the mass media, little attention has been paid to such portrayals in video games. In this descriptive study, the fifty highest-selling video games in each year from 2011 to 2013 were surveyed through application of search terms to the Wikia search engine, with subsequent review of relevant footage on YouTube. Depiction categories were then assigned based on the extent of portrayal and qualitative characteristics compared against mental illness stereotypes in cinema. Twenty-three of the 96 surveyed games depicted at least one character with mental illness. Forty-two characters were identified as portraying mental illness, with most characters classified under a "homicidal maniac" stereotype, although many characters did not clearly reflect cinema stereotypes and were subcategorized based on the shared traits. Video games contain frequent and varied portrayals of mental illness, with depictions most commonly linking mental illness to dangerous and violent behaviors. © 2016 American Academy of Forensic Sciences.

  12. Impact of customized videotape education on quality of life in patients with chronic obstructive pulmonary disease.

    Science.gov (United States)

    Petty, Thomas L; Dempsey, Edward C; Collins, Timothy; Pluss, William; Lipkus, Isaac; Cutter, Gary R; Chalmers, Robin; Mitchell, Amy; Weil, Kenneth C

    2006-01-01

    To compare the impact of a library of pulmonary rehabilitation videotapes versus an older videotape and usual care on quality of life and ability to perform activities of daily living in persons with chronic obstructive pulmonary disease. Two hundred fourteen patients diagnosed with chronic obstructive pulmonary disease, emphysema, or chronic bronchitis were recruited and randomized to receive customized videotapes, standard videotapes, or usual care. Outcome measures included the Fatigue Impact Scale, Seattle Obstructive Lung Disease Questionnaire, and the SF-36(R) Health Survey. Differences in coping skills and emotional functioning on the Seattle Obstructive Lung Disease Questionnaire were found among the 174 subjects who completed the study. The customized videotape group improved by 8.6 and 4.8 points, respectively, whereas the score of the other groups decreased by less than 1 point for the coping skills, and the scores of the standard video and the control groups decreased by 3.0 and 2.1 points, respectively, for emotional functioning (P Impact Scale also improved for the customized videotape group, whereas the scores of the others remained unchanged. Videotape users demonstrated better conversion to and retention of exercise habits, with over 80% of customized videotape subjects who reported exercise habits at baseline continuing the habits as compared with 40% in the usual care group. Sedentary subjects at baseline were more likely to begin and maintain exercise if randomized to videotapes. These findings demonstrate increased quality of life, lower fatigue, and better compliance with a prescribed exercise regimen among subjects using the customized videotapes. There was a significant improvement in emotional functioning and coping skills among customized videotape subjects.

  13. The portrayal of the menstruating woman in menstrual product advertisements.

    Science.gov (United States)

    Coutts, L B; Berg, D H

    1993-01-01

    Because menstrual product advertisements act as mediators of a subset of meanings of femininity linked to menstruation, we performed a comparative conceptual analysis of these advertisements to explicate media-constructed realities of contemporary women. We sought to understand the portrayed women's definition of menstruation and their status as menstruating women. Textual and conceptual analyses led us to conclude that the portrayed women, in an attempt to avoid others' discovery of their menstruation, employed a complex menstrual management system, which often includes feminized menstrual products, to act as an antidote to a tainted state of femininity.

  14. Suicide and the media. Part II: Portrayal in fictional media.

    Science.gov (United States)

    Pirkis, J; Blood, R W

    2001-01-01

    The association between the portrayal of suicide in fictional media and actual suicide has been debated since 1774, when it was asserted that Goethe's The Sorrows of Young Werther had led people to take their own lives. Since that time, a plethora of studies considering the association has been conducted. This review considered 34 studies examining the impact of fictional portrayal of suicide (in film and television, music, and plays) on actual suicidal behavior. It asked the question: "Is there any association, and if so, can it be considered causal?" Using strict criteria to establish causality, we found that the evidence was more equivocal than was the case for nonfictional reporting.

  15. Evolutionary Origin of the Staphylococcal Cassette Chromosome mec (SCCmec)

    DEFF Research Database (Denmark)

    Rolo, Joana; Worning, Peder; Nielsen, Jesper Boye

    2017-01-01

    Several lines of evidence indicate that the most primitive staphylococcal species, those of the Staphylococcus sciuri group, were involved in the first stages of evolution of the staphylococcal cassette chromosome mec (SCCmec), the genetic element carrying the β-lactam resistance gene mecA. Howev....... aureus clones) originated in S. sciuri possibly by a recombination event in a human host or a human-created environment and later was transferred to S. aureus....

  16. Polycistronic transcription of fused cassettes and identification of translation initiation signals in an unusual gene cassette array from Pseudomonas aeruginosa [version 3; referees: 2 approved

    Directory of Open Access Journals (Sweden)

    Érica L. Fonseca

    2015-11-01

    Full Text Available The gene cassettes found in class 1 integrons are generally promoterless units composed by an open reading frame (ORF, a short 5’ untranslated region (UTR and a 3’ recombination site (attC. Fused gene cassettes are generated by partial or total loss of the attC from the first cassette in an array, creating, in some cases, a fusion with the ORF from the next cassette. These structures are rare and little is known about their mechanisms of mobilization and expression. The aim of this study was to evaluate the dynamic of mobilization and transcription of the gcu14-blaGES-1/aacA4 gene cassette array, which harbours a fused gene cassette represented by blaGES-1/aacA4. The cassette array was analyzed by Northern blot and real-time reverse transcription-polymerase chain reaction (RT-PCR in order to assess the transcription mechanism of blaGES-1/aacA4 fused cassette. Also, inverse polymerase chain reactions (PCR were performed to detect the free circular forms of gcu14, blaGES-1 and aacA4. The Northern blot and real time RT-PCR revealed a polycistronic transcription, in which the fused cassette blaGES-1/aacA4 is transcribed as a unique gene, while gcu14 (with a canonical attC recombination site has a monocistronic transcription. The gcu14 cassette, closer to the weak configuration of cassette promoter (PcW, had a higher transcription level than blaGES-1/aacA4, indicating that the cassette position affects the transcript amounts. The presence of ORF-11 at attI1, immediately preceding gcu14, and of a Shine-Dalgarno sequence upstream blaGES-1/aacA4 composes a scenario for the occurrence of array translation. Inverse PCR generated amplicons corresponding to gcu14, gcu14-aacA4 and gcu14-blaGES-1/aacA4 free circular forms, but not to blaGES-1 and aacA4 alone, indicating that the GES-1 truncated attC is not substrate of integrase activity and that these genes are mobilized together as a unique cassette. This study was original in showing the transcription

  17. Early Book Stages, 0-5 Years [and] Creciendo con Libros (Growing [up] with Books). [Videotape.

    Science.gov (United States)

    Holguin, Roxanna

    Using a lighthearted and simple approach, this 23-minute videotape in English and Spanish versions presents interactions between parents and children while reading books. The children in the videotape range in age from 0 to 5 years. The video is introduced by scenes of children enjoying books while narration discussing the impact of reading to…

  18. Camera Perspective Bias in Videotaped Confessions: Evidence that Visual Attention Is a Mediator

    Science.gov (United States)

    Ware, Lezlee J.; Lassiter, G. Daniel; Patterson, Stephen M.; Ransom, Michael R.

    2008-01-01

    Several experiments have demonstrated a "camera perspective bias" in evaluations of videotaped confessions: videotapes with the camera focused on the suspect lead to judgments of greater voluntariness than alternative presentation formats. The present research investigated potential mediators of this bias. Using eye tracking to measure visual…

  19. Videotape Helps Standardize and Increase Objectivity of Medical Exams Across the Country

    Science.gov (United States)

    Diefenbach, Robert C.

    1976-01-01

    A 90-minute videotape demonstrates how to stage six stations simulating medical emergency problems to be solved by emergency medical technicians (EMT's) taking the National Registry's 150-question written final examination. The videotape also details the logistics of arranging the entire one-hour examination, used in over 30 States. (AJ)

  20. Multimodal Analysis of Piano Performances Portraying Different Emotions

    DEFF Research Database (Denmark)

    Jensen, Kristoffer; Frimodt-Møller, Søren

    2013-01-01

    changes, and 3 times where the music was intended to portray the emotions happy, sad and angry, respectively. Motion-capture data from all of the performances was recorded alongside the audio. We analyze differences in the data for the differ- ent emotions, both with respect to the size and shape...

  1. "Hipster Freshman": Popular Culture's Portrayal of Community College Students

    Science.gov (United States)

    Hawk, Jena L.; Hill, Lilian H.

    2016-01-01

    Due to its seemingly unclear and ambiguous mission, the community college has somewhat of a stigma attached to it, as the four-year institution defines the American college experience (LaPaglia, 1994). Although only a few studies concerning media portrayals of community college students have been published within the last 20 years, the existing…

  2. The portrayal of women in South African television commercials

    African Journals Online (AJOL)

    (2007: 409) holds that gender roles in the media are authenticated through regular exposures and then adopted by the .... Product user: People are often portrayed as product users in commercials, as television is an excellent medium for ... women than men are product users in television commercials. In a study to compare.

  3. The "Cape Times"'s Portrayal of School Violence

    Science.gov (United States)

    de Wet, Corene

    2016-01-01

    This study explores the "Cape Times"'s portrayal of school violence in the Western Cape (WC), South Africa, reporting on findings from a qualitative content analysis of 41 news articles retrieved from the SA Media database. The findings shed light on the victims and their victimisation, the perpetrators, as well as the context of the…

  4. Portrayals of Reproductive and Sexual Health on Primetime Television

    Science.gov (United States)

    Pariera, Katrina L.; Hether, Heather J.; Murphy, Sheila T.; de Castro Buffington, Sandra; Baezconde-Garbanati, Lourdes

    2013-01-01

    Primetime broadcast television provides health information and establishes norms for millions of people in the United States (Beck, 2004; Brodie, et al., 2001; Murphy & Cody, 2003; Rideout, 2008). To understand what people may be learning about reproductive and sexual health, a content analysis was conducted of storylines from the 10 most popular primetime television programs in 2009, 2010 and 2011. Variables that were measured included the frequency of reproductive and sexual health issues, the level of health information, the type of information portrayed, the gain and loss frames, the presence of stigma, the tone, and the type of role model portrayed. Eighty-seven of the 589 health storylines dealt with reproductive and sexual health, and the most common issues were pre- and post-term pregnancy complications. The majority of these storylines had a moderate or weak level of information and included specifics about treatment and symptoms but not prevention. Just over half of the issues were framed in terms of losses, meaning non-adoption of a behavior change will result in negative outcomes. Twenty-four percent of reproductive and sexual health storylines involved stigma -- usually those related to sexually transmitted infections (STIs). Most storylines were portrayed as serious and the majority of issues happened to positive role models. The implications of these portrayals for the viewing public are discussed. PMID:24156468

  5. The stereotypical portrayal of female characters in the drama Mahlo ...

    African Journals Online (AJOL)

    The aim of this paper is to examine the stereotypical portrayal of female characters in Xitsonga literature with special reference to Mahlo ya Nkwahle. The drama was written in the late 20th century. The main focus of Mahlo ya Nkwahle is on how three women influence one another to enrich themselves by killing their ...

  6. Media portrayals of older employees: A success story?

    NARCIS (Netherlands)

    van Selm, M.; van der Heijden, B. I. J. M.

    2014-01-01

    Purpose - The purpose of this paper is to provide an analysis of how portrayals of older employees in mass media messages can help combating stereotypical beliefs on their employability. Design/methodology/approach - The authors conducted a systematic review of empirical studies on mass media

  7. Media portrayals of older employees: a success story?

    NARCIS (Netherlands)

    van Selm, Martine; van der Heijden, Beatrice

    2014-01-01

    Purpose – The purpose of this paper is to provide an analysis of how portrayals of older employees in mass media messages can help combating stereotypical beliefs on their employability. Design/methodology/approach – The authors conducted a systematic review of empirical studies on mass media

  8. Portrayal of Corruption as Narrative Technique in The Beautyful ...

    African Journals Online (AJOL)

    Portrayal of Corruption as Narrative Technique in The Beautyful Ones Are Not Yet Born. ... Narration can be nuncupative when it is printed and read as fiction. ... play or film” [57]. Writing on its own has an implicit notion of finding techniques of expression that will make the work accessible. The Beautyful Ones Are Not Yet ...

  9. 25 IMAGE OF THE NIGERIA POLICE AS PORTRAYED IN GEORGIE ...

    African Journals Online (AJOL)

    Prof Alex C Asigbo

    Abstract. This article presents a literary analysis of Georgie Iotema's play,. Night Duty, which portrays a negative image of the Nigeria Police. The constitutional duty of the Nigerian police is to maintain public safety and public order. In practice, many members of the Nigeria. Police are lawbreakers. There are many instances ...

  10. The Portrayal of Masculinity in Dholuo Ohangla Music | Orwa ...

    African Journals Online (AJOL)

    It's on this premise that the paper investigates the portrayal of males in Ohangla, a genre of music where gender stereotypes are rampant. The data utilized was in the form of words and expressions downloaded from You Tube. Purposive sampling was used to get a sample of ten Ohangla songs sung by male and female ...

  11. The Cape Times's portrayal of school violence | de Wet | South ...

    African Journals Online (AJOL)

    This study explores the Cape Times's portrayal of school violence in the Western Cape (WC), South Africa, reporting on findings from a qualitative content analysis of 41 news articles retrieved from the SA Media database. The findings shed light on the victims and their victimisation, the perpetrators, as well as the context of ...

  12. The portrayal of migraine in popular music: observations and implications.

    Science.gov (United States)

    Roberts, Daniel L; Vargas, Bert B

    2012-01-01

    To describe the manner in which migraine and migaineurs are depicted in popular music. Prior studies have elucidated the ways in which the popular perception of neurological disorders is shaped by popular culture, from the inflated expectations of the prognosis of coma patients in television dramas to the association of intractable headaches with demonic possession and death by violence in the cinema. searched popular online music sites for songs with the word "migraine" in their titles. Song lyrics were studied for tone, content, and the light in which they portrayed migraine sufferers. One hundred thirty-four songs met inclusion criteria, representing the work of 126 artists. The majority of the recording artists were male (112 of 126 artists, 89%). One hundred seven of the 134 songs (80%) were recorded since 2000. Of the 79 songs that contained lyrics, 16 (20%) included explicit content; 43 (54%) make reference to hopelessness, despair, or severe pain; and 27 (34%) contained references to killing or death. Only 9 songs (11%) made any reference to successful treatment, resolution, or hope of any sort, the same number that made lyrical references to explosions or bombs. The portrayal of a disease in popular music can reflect the artist's perceptions, anxieties, and prejudices about the disease and its victims. The public, including patients, may accept these portrayals as accurate. Clinicians familiar with the portrayal of headache sufferers in cinema will not be surprised that popular musicians (both migraineurs and non-migraineurs) portray migraines as intractable, violent, and all-consuming. The lack of any balancing view is disheartening, especially in light of the advances in migraine awareness and treatment over the past decade. Perhaps the most surprising finding is that the vast majority of migraine songs are written and performed by men. © 2012 American Headache Society.

  13. 36 CFR 1280.48 - How do I apply to film, photograph, or videotape on NARA property for news purposes?

    Science.gov (United States)

    2010-07-01

    ..., photograph, or videotape on NARA property for news purposes? 1280.48 Section 1280.48 Parks, Forests, and... film, photograph, or videotape on NARA property for news purposes? (a) If you wish to film, photograph... wish to film, photograph, or videotape for news purposes at a Presidential library or at a regional...

  14. p21-ras effector domain mutants constructed by "cassette" mutagenesis

    DEFF Research Database (Denmark)

    Stone, J C; Vass, W C; Willumsen, B M

    1988-01-01

    A series of mutations encoding single-amino-acid substitutions within the v-rasH effector domain were constructed, and the ability of the mutants to induce focal transformation of NIH 3T3 cells was studied. The mutations, which spanned codons 32 to 40, were made by a "cassette" mutagenesis...... technique that involved replacing this portion of the v-rasH effector domain with a linker carrying two BspMI sites in opposite orientations. Since BspMI cleaves outside its recognition sequence, BspMI digestion of the plasmid completely removed the linker, creating a double-stranded gap whose missing ras...

  15. Is this charred material from a VHS video cassette?

    Science.gov (United States)

    Fruchtenicht, Tara; Blackledge, Robert D.; Williams, Teresa R.

    2010-06-01

    At his residence, a victim in a double homicide had installed a home-built video surveillance system. The suspects either knew of or discovered this system and removed it. In a backyard at a location associated with the suspects was a barrel used for burning trash. Could charred debris recovered from a metal bowl found among the contents of the barrel be the remains of a VHS video cassette? A positive answer to the question was obtained through a combination of optical microscopy, Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and Energy Dispersive Spectroscopy (EDS).

  16. Recombinogenic engineering of conjugative plasmids with fluorescent marker cassettes

    DEFF Research Database (Denmark)

    Reisner, A.; Molin, Søren; Zechner, E.L.

    2002-01-01

    An efficient approach for the insertion of fluorescent marker genes with sequence specificity into conjugative plasmids in Escherichia coli is described. For this purpose, homologous recombination of linear double-stranded targeting DNA was mediated by the bacteriophage lambda recombination...... resistance genes and fluorescent markers. The choice of 5' non-homologous extensions in primer pairs used for amplifying the marker cassettes determines the site specificity of the targeting DNA. This methodology is applicable to the modification of all plasmids that replicate in E coli and is not restricted...

  17. Disinfection efficacy of an ultraviolet light on film cassettes for preventive of the nosocomial infection

    Energy Technology Data Exchange (ETDEWEB)

    Kweon, Dae Cheol [Seoul National Univ. Hospital, Seoul (Korea, Republic of); Jeon, Yong Woong; Cho, Am [Dongguk Univ., Seoul (Korea, Republic of)

    2001-06-01

    The bacterial infection on film cassette contact surface was examined at the diagnostic radiology department of the S. hospital. The objective of this study was to assess the contamination level on film cassette contact surface as a predictor of patient prevention from nosocomial infection and for improvement of the hospital environment. The laboratory result was identified non-pathologic bacterial in the five different cassette size of the contact surface. Film cassettes were exposed to ultraviolet light for 1, 2 and 3 minutes. Ultraviolet light disinfection is proven suitable for bacterial. The study concludes that presence of a bacterial infection will prevent a using antiseptic technique on film cassette contact surface. In addition education of nosocomial infection for radiographers will be required. In conclusion, ultraviolet is considered effective to irradiate bacterial. Additionally, two minutes are required to sterilize film cassettes.

  18. Cassettes for solid-oxide fuel cell stacks and methods of making the same

    Science.gov (United States)

    Weil, K. Scott; Meinhardt, Kerry D; Sprenkle, Vincent L

    2012-10-23

    Solid-oxide fuel cell (SOFC) stack assembly designs are consistently investigated to develop an assembly that provides optimal performance, and durability, within desired cost parameters. A new design includes a repeat unit having a SOFC cassette and being characterized by a three-component construct. The three components include an oxidation-resistant, metal window frame hermetically joined to an electrolyte layer of a multi-layer, anode-supported ceramic cell and a pre-cassette including a separator plate having a plurality of vias that provide electrical contact between an anode-side collector within the pre-cassette and a cathode-side current collector of an adjacent cell. The third component is a cathode-side seal, which includes a standoff that supports a cathode channel spacing between each of the cassettes in a stack. Cassettes are formed by joining the pre-cassette and the window frame.

  19. Portrayal of Women’s Images in Television Dramas: A Malaysian Case

    National Research Council Canada - National Science Library

    Ibrahim, Faridah; Yunus, Norzita; Halem Shah, Dil Froz Jan Sayed; Ilias, Munirah; Ahmad Mokhtar, Amli Hazlin

    2017-01-01

    ... is their centrality in the media, through media positions held or through media images portrayed. What this paper has endeavoured to show is the portrayal of women in the media particularly, television dramas...

  20. The Portrayal of Indigenous Health in Selected Australian Media

    Directory of Open Access Journals (Sweden)

    Melissa J. Stoneham

    2014-04-01

    Full Text Available It is acknowledged that health outcomes for Australian Indigenous peoples are lower than those of non-Indigenous Australians. Research suggests negative media in relation to Indigenous Australians perpetuates racist stereotypes among the wider population and impacts on the health of Indigenous Australians. This study examined the media portrayal of Indigenous Australian public health issues in selected media over a twelve month period and found that, overwhelmingly, the articles were negative in their portrayal of Indigenous health. A total of 74 percent of the coverage of Australian Indigenous related articles were negative, 15 percent were positive, and 11 percent were neutral. The most common negative subject descriptors related to alcohol, child abuse, petrol sniffing, violence, suicide, deaths in custody, and crime.

  1. Pros and Cons of Character Portrayals of Autism on TV and Film

    Science.gov (United States)

    Nordahl-Hansen, Anders; Øien, Roald A.; Fletcher-Watson, Sue

    2018-01-01

    Portrayals of characters with autism spectrum disorder (ASD) and/or with autistic traits on film and in TV-series are increasing. Such portrayals may contribute in increasing awareness of the condition but can also increase stereotypes. Thus, these character portrayals are subject to heated debate within the ASD-community, but also in the general…

  2. Portrayals of Stepfamilies in Film: Using Media Images in Remarriage Education

    Science.gov (United States)

    Leon, Kim; Angst, Erin

    2005-01-01

    Media portrayals of stepfamilies influence societal views of stepfamilies and individuals' expectations for remarriage and stepfamily life. This study examines portrayals of stepfamilies in films released in 1990 through 2003. Using content analysis to understand how stepfamilies are portrayed and to identify film clips appropriate for use in…

  3. Computer-based desktop system for surgical videotape editing.

    Science.gov (United States)

    Vincent-Hamelin, E; Sarmiento, J M; de la Puente, J M; Vicente, M

    1997-05-01

    The educational role of surgical video presentations should be optimized by linking surgical images to graphic evaluation of indications, techniques, and results. We describe a PC-based video production system for personal editing of surgical tapes, according to the objectives of each presentation. The hardware requirement is a personal computer (100 MHz processor, 1-Gb hard disk, 16 Mb RAM) with a PC-to-TV/video transfer card plugged into a slot. Computer-generated numerical data, texts, and graphics are transformed into analog signals displayed on TV/video. A Genlock interface (a special interface card) synchronizes digital and analog signals, to overlay surgical images to electronic illustrations. The presentation is stored as digital information or recorded on a tape. The proliferation of multimedia tools is leading us to adapt presentations to the objectives of lectures and to integrate conceptual analyses with dynamic image-based information. We describe a system that handles both digital and analog signals, production being recorded on a tape. Movies may be managed in a digital environment, with either an "on-line" or "off-line" approach. System requirements are high, but handling a single device optimizes editing without incurring such complexity that management becomes impractical to surgeons. Our experience suggests that computerized editing allows linking surgical scientific and didactic messages on a single communication medium, either a videotape or a CD-ROM.

  4. Artaud's Journey to Mexico and His Portrayals of the Land

    OpenAIRE

    Su, Tsu-Chung

    2012-01-01

    In his article "Artaud's Journey to Mexico and His Portrayals of the Land" Tsu-Chung Su examines Artaud's visions, visualizations, descriptions, and conceptualizations of Mexico. Su argues that Artaud's writings about Mexico were his textual appropriations and cartographical remappings of the land. They embodied both the geographic wandering of his itinerary and the bodily spasms of his thought. At once geographical and psycho-physiological embodiments, they were not only texts of a questing ...

  5. The Portrayal of Indigenous Health in Selected Australian Media

    OpenAIRE

    Melissa J. Stoneham; Jodie Goodman; Mike Daube

    2014-01-01

    It is acknowledged that health outcomes for Australian Indigenous peoples are lower than those of non-Indigenous Australians. Research suggests negative media in relation to Indigenous Australians perpetuates racist stereotypes among the wider population and impacts on the health of Indigenous Australians. This study examined the media portrayal of Indigenous Australian public health issues in selected media over a twelve month period and found that, overwhelmingly, the articles were negative...

  6. An AC electrokinetics facilitated biosensor cassette for rapid pathogen identification.

    Science.gov (United States)

    Ouyang, Mengxing; Mohan, Ruchika; Lu, Yi; Liu, Tingting; Mach, Kathleen E; Sin, Mandy L Y; McComb, Mason; Joshi, Janhvi; Gau, Vincent; Wong, Pak Kin; Liao, Joseph C

    2013-07-07

    To develop a portable point-of-care system based on biosensors for common infectious diseases such as urinary tract infection, the sensing process needs to be implemented within an enclosed fluidic system. On chip sample preparation of clinical samples remains a significant obstacle to achieving robust sensor performance. Herein AC electrokinetics is applied in an electrochemical biosensor cassette to enhance molecular convection and hybridization efficiency through electrokinetics induced fluid motion and Joule heating induced temperature elevation. Using E. coli as an exemplary pathogen, we determined the optimal electrokinetic parameters for detecting bacterial 16S rRNA in the biosensor cassette based on the current output, signal-to-noise ratio, and limit of detection. In addition, a panel of six probe sets targeting common uropathogenic bacteria was demonstrated. The optimized parameters were also validated using patient-derived clinical urine samples. The effectiveness of electrokinetics for on chip sample preparation will facilitate the implementation of point-of-care diagnosis of urinary tract infection in the future.

  7. AC Electrokinetics Facilitated Biosensor Cassette for Rapid Pathogen Identification

    Science.gov (United States)

    Ouyang, Mengxing; Mohan, Ruchika; Lu, Yi; Liu, Tingting; Mach, Kathleen E.; Sin, Mandy L. Y.; McComb, Mason; Joshi, Janhvi; Gau, Vincent

    2013-01-01

    To develop a portable point-of-care system based on biosensors for common infectious diseases such as urinary tract infection, the sensing process needs to be implemented within an enclosed fluidic system. On chip sample preparation of clinical samples remains a significant obstacle to achieve robust sensor performance. Herein AC electrokinetics is applied in an electrochemical biosensor cassette to enhance molecular convection and hybridization efficiency though electrokinetic induced fluid motion and Joule heating induced temperature elevation. Using E. coli as an exemplary pathogen, we determined the optimal electrokinetic parameters for detecting bacterial 16S rRNA in the biosensor cassette based on the current output, signal-to-noise ratio, and limit of detection. In addition, a panel of six probe sets targeting common uropathogenic bacteria was demonstrated. The optimized parameters were also validated using patient-derived clinical urine samples. The effectiveness of electrokinetic for on chip sample preparation will facilitate the implementation of point-of-care diagnosis of urinary tract infection in the future. PMID:23626988

  8. [The portrayal of multiple sclerosis in television series].

    Science.gov (United States)

    Karenberg, A

    2009-04-01

    An increasing number of television series deal with neurological disorders, including fictional portrayals of multiple sclerosis (MS). The aim of this paper was to analyze every available TV episode with an MS character. Productions were identified by film databases and by hand search. Each episode was evaluated along neurologic and cinematic lines. Between 1985 and 2006, portrayals of MS appeared in 17 episodes produced in Germany, the US, and the UK. The frequency of symptoms shown onscreen strongly differed from epidemiological data. In particular sensory, cognitive, and bladder symptoms as well as difficulties with sexual function were under-represented. The authenticity of the disease depiction was strongly dependent upon the genre. Coping stories could be identified as the most prominent genre. Television patients were often portrayed as "brave fighters", "refined characters", and "afflicted without symptoms". Television series attract millions of viewers and thus shape the public image of a disease. Sound knowledge of how symptoms, diagnosis, and therapeutic options are presented in mass media is therefore indispensable for all who deal with MS patients, relatives, and caregivers.

  9. Media portrayal of older people as illustrated in Finnish newspapers.

    Science.gov (United States)

    Koskinen, Sanna; Salminen, Leena; Leino-Kilpi, Helena

    2014-01-01

    Media portrayals of older people, such as those in newspapers, both inform and reflect public attitudes. By becoming aware of culturally influenced attitudes about older people, and how these attitudes are reflected in the ways older people are viewed, treated, and cared for in society, the healthcare profession can better understand how to provide high-quality care. By applying an ethnographic approach in textual reality, this paper explores how newspaper articles focusing on health portray older people in society, using Finland as an example. The data consist of articles selected from three of the main Finnish daily newspapers during a 3-month period in the spring of 2012. The findings show that, overall, the society regards older people and their care as important. However, there were suggestions of paternalistic attitudes towards older people. Furthermore, the perceptions regarding different groups of older people could lead to the possibility of inequality. The media portrayals of older people worldwide seem to share similarities, although the findings of this study are particularly in accordance with the cultural attributes of the Nordic countries and societies.

  10. Media portrayal of older people as illustrated in Finnish newspapers

    Directory of Open Access Journals (Sweden)

    Sanna Koskinen

    2014-09-01

    Full Text Available Media portrayals of older people, such as those in newspapers, both inform and reflect public attitudes. By becoming aware of culturally influenced attitudes about older people, and how these attitudes are reflected in the ways older people are viewed, treated, and cared for in society, the healthcare profession can better understand how to provide high-quality care. By applying an ethnographic approach in textual reality, this paper explores how newspaper articles focusing on health portray older people in society, using Finland as an example. The data consist of articles selected from three of the main Finnish daily newspapers during a 3-month period in the spring of 2012. The findings show that, overall, the society regards older people and their care as important. However, there were suggestions of paternalistic attitudes towards older people. Furthermore, the perceptions regarding different groups of older people could lead to the possibility of inequality. The media portrayals of older people worldwide seem to share similarities, although the findings of this study are particularly in accordance with the cultural attributes of the Nordic countries and societies.

  11. Extracted image analysis: a technique for deciphering mediated portrayals.

    Science.gov (United States)

    Berg, D H; Coutts, L B

    1995-01-01

    A technique for analyzing print media that we have developed as a consequence of our interest in the portrayal of women in menstrual product advertising is reported. The technique, which we call extracted image analysis, involves a unique application of grounded theory and the concomitant heuristic use of the concept of ideal type (Weber, 1958). It provides a means of heuristically conceptualizing the answer to a variant of the "What is going on here?" question asked in analysis of print communication, that is, "Who is being portrayed/addressed here?" Extracted image analysis involves the use of grounded theory to develop ideal typologies. Because the technique re-constructs the ideal types embedded in a communication, it possesses considerable potential as a means of identifying the profiles of members of identifiable groups held by the producers of the directed messages. In addition, the analysis of such portrayals over time would be particularly well suited to extracted image analysis. A number of other possible applications are also suggested.

  12. The portrayal of mental health in Australian daily newspapers.

    Science.gov (United States)

    Kenez, Stephanie; O'Halloran, Paul; Liamputtong, Pranee

    2015-12-01

    To examine how mental health is portrayed as a holistic construct in three major Australian daily newspapers. Using an unobtrusive, mixed methods approach incorporating content and thematic analyses, data were collected from hard copies of The Age, The Australian and Herald Sun newspapers and their weekend counterparts over 12 weeks in 2012. Newspaper coverage of mental health favoured stories about illness over wellbeing. While the issue was typically reported responsibly, psychotic disorders were overrepresented in discussions of illness and were often discussed in relation to deviance. Our analysis suggested that positive mental health messages are commonly presented, particularly in tabloid coverage, which appears to place greater emphasis on wellbeing. However, improvements in the representation of psychotic disorders appear to be less progressive than other disorders. This suggests mental health professionals and organisations need to continue building relationships with and providing education to journalists to ensure responsible representations continue to dominate. This study provides an updated and holistic examination of the media's portrayal of mental health within the Australian context. As this is the first study to examine the portrayal of wellbeing and illness, it may provide insight into how media representations of mental health affect society beyond those affected by illness. © 2015 Public Health Association of Australia.

  13. Media Portrayals of Female Perpetrators of Intimate Partner Violence.

    Science.gov (United States)

    Carlyle, Kellie E; Scarduzio, Jennifer A; Slater, Michael D

    2014-09-01

    Preventing intimate partner violence (IPV) is a public health priority. An important component of designing prevention programs is developing an understanding of how media portrayals of health issues influence public opinion and policy. To better understand the ways in which media images may be informing our understanding of IPV, this study content analyzed portrayals of IPV in news media articles. Stratified media outlets were used to obtain a representative sample of daily newspapers based on their designated market areas. Researchers created constructed months using weeks from each season across a 2-year period. The first part of the study investigated quantitative differences in the coverage of female and male perpetrators (n = 395) and identified several areas where coverage differed. The second part of the study qualitatively examined coverage of female perpetrators (n = 61) to provide a richer description of such coverage. This study contributes to our understanding of female perpetrators and how these portrayals may contribute to the larger gender symmetry debate surrounding female aggressors. Implications for public health policy and research are discussed. © The Author(s) 2014.

  14. Videotape review: a valuable tool for self-assessment of teaching skills.

    Science.gov (United States)

    Kleffner, J H; Hendricson, W D; Littlefield, J H; Hudepohl, N

    1985-11-01

    This paper describes the components of an instructor improvement program known as VISIT (Videotaping Instruction for Self-Assessment of Instructional Technique). Through the use of videotape recordings of classroom, laboratory or clinical teaching and observation by trained observers, faculty can pinpoint instructional strengths and problem areas, plan refinements and evaluate the impact of these refinements. Ten years of experience with VISIT indicates that post-lecture consultation sessions between the instructor and observer are vital to the success of this activity.

  15. A survey of the radiographic cassettes disinfection of university hospitals in seoul

    Energy Technology Data Exchange (ETDEWEB)

    Kweon, Dae Cheol; Park, Peom [College of School, Ajou Univ., Suwon (Korea, Republic of); Kim, Moon Sun; Kim, Dong Sung [College of Medicine, Seoul National Univ., Seoul (Korea, Republic of)

    2001-04-01

    The purpose of this study is to prevent nosocomial infection in patients through contact of radiographic cassettes. Data were collected from radiographers working in 29 university hospitals in Seoul in February and March 2001. Radiographic cassettes were disinfected daily in 5 hospitals, weekly in 4 hospitals, monthly in 5 hospitals, bimonthly in 1 hospital and once every three months in another hospital. 12 other hospitals do not practice regular disinfections of radiographic cassettes. Gauze soaked in disinfectant solution is used in 7 hospitals while 11 hospitals used cotton and cloth soaked in disinfectant solution to clean the radiographic cassettes. 26 hospitals used 99% alcohol based disinfectant solutions while 3 hospitals used 75% alcohol based disinfectant, 26 hospitals use of intercourse cassettes outpatients and in patients. In 26 hospitals, all patients shared the same set of radiographic cassettes used in the hospitals, or in 26 hospitals, separate sets of radiographic cassettes are used for outpatients and inpatients. Separate sets of cassettes are used for ICU and inpatients in 6 others hospitals. 23 hospitals used the same sets of radiographic cassettes for all their patients. radiographic cassettes are cleaned in wash area in the study room of the radiographic department in 17 hospitals. 12 other hospitals do not have designated cleaning areas for the cassettes. All radiographers practiced hands washing with soap. All 29 hospitals surveyed have infection control committee. However, only 9 out of the 29 hospitals surveyed provided Infection {center_dot} disinfections control education to radiographers. Only 3 hospitals have radiographers sitting in the infection control committee. Infection management education is conducted in 63 hospitals annually, twice a year in 1 hospital and once every 3 months in 2 hospitals.

  16. An investigation of infection control for x-ray cassettes in a diagnostic imaging department

    Energy Technology Data Exchange (ETDEWEB)

    Fox, Matthew [School of Allied Health Professions and Science, Faculty of Health, Wellbeing and Science, University Campus Suffolk, Rope Walk, Ipswich, Suffolk, IP4 1LT (United Kingdom); Harvey, Jane M. [School of Allied Health Professions and Science, Faculty of Health, Wellbeing and Science, University Campus Suffolk, Rope Walk, Ipswich, Suffolk, IP4 1LT (United Kingdom)], E-mail: j.harvey@ucs.ac.uk

    2008-11-15

    Introduction: This research was conducted to investigate if X-ray cassettes could be a possible source of pathogens capable of causing nosocomial infections, and if they could be a possible vector for cross infection within the hospital environment. Method: The research involved the swabbing of X-ray cassettes in a Diagnostic Imaging Department of a large hospital in the east of England. Two areas of the Diagnostic Imaging Department were included in the study. Research concentrated on X-ray cassettes used for mobile radiography, accident and emergency and inpatient use. Forty cassettes were swabbed in total specifically for general levels of bacterial contamination, also for the presence or absence of methicillin-resistant Staphylococcus aureus (MRSA). A mapping exercise was completed following the location of an X-ray cassette typically used in mobile radiography. The exercise noted the level of direct contact with patient's skin and other possible routes of infection. Results: The results demonstrated that there were large levels of growth of samples taken from cassettes and developed in the Microbiology Department. Coagulase-negative Staphylococcus, Micrococci, Diptheroids and species of Bacillus were all identified. The mapping exercise in which the journey of a 35/43 cm cassette used for mobile radiography was tracked found that contact with patient's skin and potential pathogens or routes of cross infection was a common occurrence whilst undertaking mobile radiography. Conclusion: The research has identified the presence of bacterial contamination on cassettes. The research established that X-ray cassettes/imaging plates are often exposed to pathogens and possible routes of cross infection; also that patient's skin often comes directly in contact with the X-ray cassette/imaging plate. The research also shows that as cassettes/imaging plates are a potential source of cross infection, the Diagnostic Imaging Department may be partly responsible

  17. Portrayal of smokeless tobacco in YouTube videos.

    Science.gov (United States)

    Bromberg, Julie E; Augustson, Erik M; Backinger, Cathy L

    2012-04-01

    Videos of smokeless tobacco (ST) on YouTube are abundant and easily accessible, yet no studies have examined the content of ST videos. This study assesses the overall portrayal, genre, and messages of ST YouTube videos. In August 2010, researchers identified the top 20 search results on YouTube by "relevance" and "view count" for the following search terms: "ST," "chewing tobacco," "snus," and "Skoal." After eliminating videos that were not about ST (n = 26), non-English (n = 14), or duplicate (n = 42), a final sample of 78 unique videos was coded for overall portrayal, genre, and various content measures. Among the 78 unique videos, 15.4% were anti-ST, while 74.4% were pro-ST. Researchers were unable to determine the portrayal of ST in the remaining 10.3% of videos because they involved excessive or "sensationalized" use of the ST, which could be interpreted either positively or negatively, depending on the viewer. The most common ST genre was positive video diaries (or "vlogs"), which made up almost one third of the videos (29.5%), followed by promotional advertisements (20.5%) and anti-ST public service announcements (12.8%). While YouTube is intended for user-generated content, 23.1% of the videos were created by professional organizations. These results demonstrate that ST videos on YouTube are overwhelmingly pro-ST. More research is needed to determine who is viewing these ST YouTube videos and how they may affect people's knowledge, attitudes, and behaviors regarding ST use.

  18. Survivors on Cancer: the portrayal of survivors in print news.

    Science.gov (United States)

    Kromm, Elizabeth Edsall; Smith, Katherine Clegg; Singer, Rachel Friedman

    2007-12-01

    This study examines the types of news stories that include comments by everyday cancer survivors and the messages or information these individuals provide. Even though these non-celebrity survivors increasingly serve on the front lines of cancer prevention and advocacy efforts and often engage with media, the role they play in the media discourse on cancer has not been a focus of research. We conducted a thematic content analysis of print news articles of non-celebrity cancer survivors in 15 leading national daily newspapers for four consecutive months starting in June 2005 to identify the issues or events that included a survivor perspective and the messages or information conveyed by the everyday survivors. Journalists included survivor commentary primarily when covering cancer fundraising events and when focusing on individual survivorship stories. In overall news coverage involving survivors, breast and prostate cancers received the greatest attention, followed by blood and lung cancers. Survivors spoke mainly about the diagnosis experience and life post-cancer. Our analysis of survivors' comments revealed that discussions of the diagnosis experience often convey fear and a lack of confidence in cancer screening practices, while cancer is portrayed as a positive life event. While evidence of a positive and hopeful portrayal of survivorship is an encouraging finding for continued efforts to decrease stigma associated with a cancer diagnosis and for the public understanding of the disease, it is important to consider potential negative implications of an idealized and restricted media discourse on survivorship. The increasing size and capacity of the survivor community offers opportunities for the cancer advocacy community to consider how news media portrayal of cancer and survivorship may contribute in both positive and potentially detrimental ways to public understanding of this disease, its survivors and life after cancer.

  19. Media portrayal of elite athletes with disability - a systematic review.

    Science.gov (United States)

    Rees, Leanne; Robinson, Priscilla; Shields, Nora

    2017-11-10

    The media plays an important role in shaping society's beliefs about disability and sport. The aim of this systematic review is to identify how elite athletes with disability are portrayed in the media. Six electronic databases were searched from 2001 to March 2017 for quantitative or qualitative content analysis of media coverage of elite athletes with disability: SportsDiscus, CINAHL, PsychInfo, Medline 1996-, Embase, and Proquest. Quality assessment and data extraction were performed by two independent assessors. Seventeen moderate quality articles were included. Six themes emerged from the data such as frequency of articles and photos about elite athletes with disability; athlete gender; athlete nationality; disability; athleticism; and Olympic Games versus Paralympic Games. Our results show that elite athletes with disability are less visible in the media than their nondisabled counterparts; female athletes received less coverage than male; the media favored domestic athletes and certain types of disability; and, although there was a focus on athleticism, this was underpinned by a "supercrip" narrative and a medicalised description of disability. Although there has been a positive shift in the narrative around elite athletes with disability in media, relative absence and differing portrayal is present. Considering the power of media shaping society's perceptions of disability, further investigation is warranted. Implications for Rehabilitation Media has a role in how elite athletes with disability are portrayed and consequently perceived by the public. Elite athletes with disability rarely feature in media. Images of disability are minimized, and certain types of disabilities are favored. An athletic narrative is emerging; however, a medicalised description of athletes remains, shifting the focus from athleticism. "Supercrip" and "Superhuman" terms are commonly used, but may negatively impact the broader disability community.

  20. Portrayal of electronic cigarettes on YouTube

    OpenAIRE

    Luo, Chuan; Zheng, Xiaolong; Zeng, Daniel Dajun; Leischow, Scott

    2014-01-01

    Background As the most popular video sharing website in the world, YouTube has the potential to reach and influence a huge audience. This study aims to gain a systematic understanding of what e-cigarette messages people are being exposed to on YouTube by assessing the quantity, portrayal and reach of e-cigarette videos. Methods Researchers identified the top 20 search results on YouTube by relevance and view count for the following search terms: “electronic cigarettes”, “e-cigarettes”, “eciga...

  1. Fake news portrayals of stem cells and stem cell research.

    Science.gov (United States)

    Marcon, Alessandro R; Murdoch, Blake; Caulfield, Timothy

    2017-10-01

    This study examines how stem cells and stem cell research are portrayed on websites deemed to be purveyors of distorted and dubious information. Content analysis was conducted on 224 articles from 2015 to 2016, compiled by searching with the keywords 'stem cell(s)' on a list of websites flagged for containing either 'fake' or 'junk science' news. Articles contained various exaggerated positive and negative claims about stem cells and stem cell science, health and science related conspiracy theories, and statements promoting fear and mistrust of conventional medicine. Findings demonstrate the existence of organized misinformation networks, which may lead the public away from accurate information and facilitate a polarization of public discourse.

  2. Prodigal daughters: portraying lesbians in Hispanic Caribbean cinema.

    Science.gov (United States)

    Martinez-Reyes, Consuelo

    2012-01-01

    During the last twenty years, Hispanic Caribbean cinema has slowly developed roles to represent lesbians. In order to draw a conceptual map and to examine the un/successfulness of this new lesbian "public image," I analyze both independent films that challenge the status quo by portraying openly lesbian characters and mainstream films that insist on denying autonomy to same-sex love. Whereas commercial markets may deem an openly lesbian role transgressive, queer female roles can be considered "appropriate." Gender-queering functions as a symbolically transitional stage toward lesbian visibility and inclusion.

  3. The effect of positive and negative movie alcohol portrayals on transportation and attitude toward the movie.

    Science.gov (United States)

    Koordeman, Renske; Anschutz, Doeschka J; Engels, Rutger C M E

    2014-07-01

    This study examined the effects of alcohol portrayals on transportation and attitude toward a movie. In addition, we examined whether positive and negative movie alcohol portrayals affect transportation into and attitude toward the movie. A within-subject design was used in which participants were exposed to 8 different movie clips containing alcohol (positive or negative context) or no alcohol portrayals in a controlled laboratory setting. A total of 159 college students (84 males and 75 females) ages 18 to 30 participated in the experiment. Transportation and attitude toward the movie were measured after each movie clip. Participants were more transported into and had a more positive attitude toward movie clips with alcohol portrayals compared to the same movie clips with no alcohol portrayal. In addition, participants were more transported into movie clips with negative alcohol (NA) portrayals compared to clips with positive alcohol (PA) portrayals. For attitude toward the movie, opposite results were found. Participants had a more positive attitudes toward clips with PA portrayals compared to clips with NA portrayals. The way alcohol is portrayed in movies may contribute to how people evaluate and get transported in movies. Copyright © 2014 by the Research Society on Alcoholism.

  4. A two-cassette reporter system for assessing target gene translation and target gene product inclusion body formation

    DEFF Research Database (Denmark)

    2016-01-01

    The present invention relates to a dual cassette reporter system capable of assessing target gene translation and target gene product folding. The present invention further relates to vectors and host cells comprising the dual cassette reporter system. In addition the invention relates to the use...... of the dual cassette reporter system for assessing target gene translation and target gene product folding....

  5. Media-portrayed idealized images, body shame, and appearance anxiety.

    Science.gov (United States)

    Monro, Fiona; Huon, Gail

    2005-07-01

    This study was designed to determine the effects of media-portrayed idealized images on young women's body shame and appearance anxiety, and to establish whether the effects depend on advertisement type and on participant self-objectification. Participants were 39 female university students. Twenty-four magazine advertisements comprised 12 body-related and 12 non-body-related products, one half of each with, and the other one half without, idealized images. Preexposure and post exposure body shame and appearance anxiety measures were recorded. Appearance anxiety increased after viewing advertisements featuring idealized images. There was also a significant interaction between self-objectification level and idealized body (presence vs. absence). No differences emerged for body-related compared with non-body-related product advertisements. The only result for body shame was a main effect for time. Participants' body shame increased after exposure to idealized images, irrespective of advertisement type. Although our findings reveal that media-portrayed idealized images detrimentally affect the body image of young women, they highlight the individual differences in vulnerability and the different effects for different components of body image. These results are discussed in terms of their implications for the prevention and early intervention of body image and dieting-related disorders. ( Copyright 2005 by Wiley Periodicals, Inc

  6. Producing Videotape Programs for Computer Training: An Example with AMA/NET

    Science.gov (United States)

    Novey, Donald W.

    1990-01-01

    To facilitate user proficiency with AMA/Net, an 80-minute training videotape has been produced. The production was designed to use videotape's advantages, where information and emotion are combined; and to accommodate its chief disadvantage, lack of resolution for fine text, with close-ups and graphics. Content of the videotape was conceived, outlined, demonstrated with simultaneous text capture, edited into script form, narration added, and scripts marked for videotaping and narrating. Videotaping was performed with actual keyboard sounds for realism. The recording was divided into four areas: office mock-up, keyboard close-ups, scan-conversion and screen close-ups. Once the footage was recorded, it was logged and rough-edited. Care was taken to balance the pace of the program with visual stimulation and amount of narration. The final edit was performed as a culmination of all scripts, video materials and rough edit, with graphics and steady change of visual information offsetting the static nature of the screen display. Carefully planned video programs can be a useful and economical adjunct in the training process for online services.

  7. Context-driven discovery of gene cassettes in mobile integrons using a computational grammar.

    Science.gov (United States)

    Tsafnat, Guy; Coiera, Enrico; Partridge, Sally R; Schaeffer, Jaron; Iredell, Jon R

    2009-09-08

    Gene discovery algorithms typically examine sequence data for low level patterns. A novel method to computationally discover higher order DNA structures is presented, using a context sensitive grammar. The algorithm was applied to the discovery of gene cassettes associated with integrons. The discovery and annotation of antibiotic resistance genes in such cassettes is essential for effective monitoring of antibiotic resistance patterns and formulation of public health antibiotic prescription policies. We discovered two new putative gene cassettes using the method, from 276 integron features and 978 GenBank sequences. The system achieved kappa = 0.972 annotation agreement with an expert gold standard of 300 sequences. In rediscovery experiments, we deleted 789,196 cassette instances over 2030 experiments and correctly relabelled 85.6% (alpha > or = 95%, E analysis demonstrated that for 72,338 missed deletions, two adjacent deleted cassettes were labeled as a single cassette, increasing performance to 94.8% (mean sensitivity = 0.92, specificity = 1, F-score = 0.96). Using grammars we were able to represent heuristic background knowledge about large and complex structures in DNA. Importantly, we were also able to use the context embedded in the model to discover new putative antibiotic resistance gene cassettes. The method is complementary to existing automatic annotation systems which operate at the sequence level.

  8. Context-driven discovery of gene cassettes in mobile integrons using a computational grammar

    Directory of Open Access Journals (Sweden)

    Schaeffer Jaron

    2009-09-01

    Full Text Available Abstract Background Gene discovery algorithms typically examine sequence data for low level patterns. A novel method to computationally discover higher order DNA structures is presented, using a context sensitive grammar. The algorithm was applied to the discovery of gene cassettes associated with integrons. The discovery and annotation of antibiotic resistance genes in such cassettes is essential for effective monitoring of antibiotic resistance patterns and formulation of public health antibiotic prescription policies. Results We discovered two new putative gene cassettes using the method, from 276 integron features and 978 GenBank sequences. The system achieved κ = 0.972 annotation agreement with an expert gold standard of 300 sequences. In rediscovery experiments, we deleted 789,196 cassette instances over 2030 experiments and correctly relabelled 85.6% (α ≥ 95%, E ≤ 1%, mean sensitivity = 0.86, specificity = 1, F-score = 0.93, with no false positives. Error analysis demonstrated that for 72,338 missed deletions, two adjacent deleted cassettes were labeled as a single cassette, increasing performance to 94.8% (mean sensitivity = 0.92, specificity = 1, F-score = 0.96. Conclusion Using grammars we were able to represent heuristic background knowledge about large and complex structures in DNA. Importantly, we were also able to use the context embedded in the model to discover new putative antibiotic resistance gene cassettes. The method is complementary to existing automatic annotation systems which operate at the sequence level.

  9. [Competent and diverse. Portrayal of older adults in Dutch television commercials ten years later].

    Science.gov (United States)

    van Selm, M; Westerhof, G J; de Vos, B

    2007-05-01

    The present study replicates our study of older adults' portrayal in Dutch television commercials conducted in 1993. The central question is whether older adults are being portrayed more visibly in Dutch television commercials and whether this portrayal has become more diverse compared to ten years ago. Based on a list of descriptions of all commercials broadcasted by public television channels in 2003 (N= 4767) 117 commercials featuring older adults were selected. By means of a quantitative content analysis it was examined whether and how older men and women are portrayed. It was concluded that although older adults are not more prevalent compared to ten years ago, their portrayal is more diverse with respect to their roles and the advertised products. Older adults were portrayed as more competent and less age-stereotypical in television commercials.

  10. Portrayals of men and women in Drum magazine (South Africa) advertisements

    OpenAIRE

    Mlenga Jere

    2014-01-01

    Orientation: The subject of gender portrayals in advertising continues to generate academicdiscussions in part because of its socialisation effects.Research purpose: The purpose of this study is to determine how print advertisements inDrum magazine portray women and men based on a number of categories including traitdescriptors, physical characteristics, role behaviours and occupational status.Motivation for the study: It is important to understand gender portrayals in advertisingbecause perc...

  11. A clinical trial of a rare earth screen/film system in a periapical cassette

    Energy Technology Data Exchange (ETDEWEB)

    Kogon, S.L.; Stephens, R.G.; Reid, J.A.; Lubus, N.J.

    1984-04-01

    In a clinical trial, a slow rare earth screen/film system (Siemens Titan 2D/Kodak XG) was used to obtain intraoral radiographs at conventional monitoring stages in endodontic treatment. The screen film image proved to be an effective substitute for the direct-exposure Ultraspeed periapical film. The intraoral cassettes, designed and fabricated for the study, were an adaptation of the flexible, vacuum-sealed cassettes used in mammography. It is believed that when a practicable periapical cassette is manufactured, many additional indications for the system are probable. Major reductions in patient exposure of at least 85% to 90% per periapical film would be effected.

  12. The Portrayal of White Organized Racists Characters on Television : How White Organized Racist Characters are Portrayed in Oz, Sons of Anarchy, and Justified

    OpenAIRE

    Halvorsen, Gina B

    2012-01-01

    The purpose of this thesis is to look at how white organized racist characters are portrayed on television by taking a closer look at the white organized racist characters portrayed in Oz, Sons of Anarchy and Justified. It will look closer at how the social class and social status of these characters have been defined and portrayed. It will also look at how the white organized racist characters perform their gender and what types of masculinity they display, and analyze the usage of racist sy...

  13. Childhood videotaped social and neuromotor precursors of schizophrenia: a prospective investigation

    DEFF Research Database (Denmark)

    Schiffman, Jason; Walker, Elaine; Ekstrøm, Morten

    2004-01-01

    OBJECTIVE: The authors examined videotaped behaviors of children who developed schizophrenia as adults and of comparison subjects to disclose possible social and neuromotor deficits foreshadowing later development of schizophrenia. METHOD: In 1972, a sample of 265 11-13-year-old Danish children...... disorder. In 1991, adult psychiatric outcome data were obtained for 91.3% (N=242). This study systematically analyzed the videotapes to determine whether the children who developed schizophrenia as adults evidenced greater social and/or neuromotor deficits than children who did not develop a psychiatric...... disorder and children who developed other psychiatric disorders. RESULTS: The findings from this study suggest that the brief videotaped footage of children eating lunch was able to discriminate between the individuals who later developed schizophrenia and those who did not. Specifically...

  14. Human ATP-binding cassette (ABC transporter family

    Directory of Open Access Journals (Sweden)

    Vasiliou Vasilis

    2009-04-01

    Full Text Available Abstract There exist four fundamentally different classes of membrane-bound transport proteins: ion channels; transporters; aquaporins; and ATP-powered pumps. ATP-binding cassette (ABC transporters are an example of ATP-dependent pumps. ABC transporters are ubiquitous membrane-bound proteins, present in all prokaryotes, as well as plants, fungi, yeast and animals. These pumps can move substrates in (influx or out (efflux of cells. In mammals, ABC transporters are expressed predominantly in the liver, intestine, blood-brain barrier, blood-testis barrier, placenta and kidney. ABC proteins transport a number of endogenous substrates, including inorganic anions, metal ions, peptides, amino acids, sugars and a large number of hydrophobic compounds and metabolites across the plasma membrane, and also across intracellular membranes. The human genome contains 49 ABC genes, arranged in eight subfamilies and named via divergent evolution. That ABC genes are important is underscored by the fact that mutations in at least I I of these genes are already known to cause severe inherited diseases (eg cystic fibrosis and X-linked adrenoleukodystrophy [X-ALD]. ABC transporters also participate in the movement of most drugs and their metabolites across cell surface and cellular organelle membranes; thus, defects in these genes can be important in terms of cancer therapy, pharmacokinetics and innumerable pharmacogenetic disorders.

  15. Pharmacogenetics of ATP-binding cassette transporters and clinical implications.

    Science.gov (United States)

    Cascorbi, Ingolf; Haenisch, Sierk

    2010-01-01

    Drug resistance is a severe limitation of chemotherapy of various malignancies. In particular efflux transporters of the ATP-binding cassette family such as ABCB1 (P-glycoprotein), the ABCC (multidrug resistance-associated protein) family, and ABCG2 (breast cancer resistance protein) have been identified as major determinants of chemoresistance in tumor cells. Bioavailability depends not only on the activity of drug metabolizing enzymes but also to a major extent on the activity of drug transport across biomembranes. They are expressed in the apical membranes of many barrier tissues such as the intestine, liver, blood-brain barrier, kidney, placenta, testis, and in lymphocytes, thus contributing to plasma, liquor, but also intracellular drug disposition. Since expression and function exhibit a broad variability, it was hypothesized that hereditary variances in the genes of membrane transporters could explain at least in part interindividual differences of pharmacokinetics of a variety of anticancer drugs and many others contributing to the clinical outcome of certain leukemias and further malignancies.

  16. Evaluating first-year nursing students' ability to self-assess psychomotor skills using videotape.

    Science.gov (United States)

    Watts, Wilda Ellen; Rush, Kathy; Wright, Marjorie

    2009-01-01

    Developing confidence in self-assessment is an important skill in becoming a self-regulated learner. This article describes the process undertaken by a group of educators of incorporating self-assessment in combination with psychomotor skill development with freshman students. Students were videotaped performing a wound-dressing change; the videotaping was immediately followed by a self-assessment of their performance using a faculty-generated checklist. Comparison of faculty and student ratings revealed the tendency for students to overrate their performance and identified discordance between students and faculty on several steps of the procedure. These evaluation findings are discussed and future directions explored.

  17. A Content Analysis of Electronic Cigarette Portrayal in Newspapers.

    Science.gov (United States)

    Yates, Katherine; Friedman, Katherine; Slater, Michael D; Berman, Micah; Paskett, Electra D; Ferketich, Amy K

    2015-04-01

    To determine how electronic cigarettes (e-cigarettes) are portrayed in newspaper informative articles and opinion pieces. A content analysis was conducted on 450 articles published in the United States from 1997 to mid-2014 and obtained by a Newsbank search. The articles were reliably coded for overall frame, type of article, first topic and main topics addressed. The article topics have changed over time and suggest significant differences between news articles and opinion pieces. Informative articles focused on e-cigarette regulation, while opinion pieces highlighted their increasing popularity and perceived health benefits. This content analysis uncovered significant interest in e-cigarettes, particularly in their regulation. The FDA should consider public perceptions of e-cigarettes when developing regulations.

  18. Portrayal of women as intimate partner domestic violence perpetrators.

    Science.gov (United States)

    Hester, Marianne

    2012-09-01

    The article explores some of the ways heterosexual women are portrayed as perpetrators of intimate partner domestic violence (IPV) in police domestic violence records in England and is the first study in the United Kingdom to examine the issue of gender and domestic violence perpetrators in any detail and over time. The article is based on a study of 128 IPV cases tracked longitudinally over 6 years, including 32 cases where women were the sole perpetrators and a further 32 cases where women were "dual" perpetrators alongside men. Women were 3 times more likely than men to be arrested when they were construed as the perpetrator. However, Pence and Dasgupta's category of "pathological violence" appeared more useful as an analytical category in the construction of women as "perpetrators" and men as "victims" than the notion of "battering."

  19. Stem cell hype: media portrayal of therapy translation.

    Science.gov (United States)

    Kamenova, Kalina; Caulfield, Timothy

    2015-03-11

    In this Perspective, we examine the portrayal of translational stem cell research in major daily newspapers in Canada, the United States, and the United Kingdom between 2010 and 2013, focusing on how timelines for stem cell therapies were represented before and after Geron terminated its pioneering stem cell program. Our content analysis reveals that press coverage has shifted from ethical, legal, and social issues to clinical translation issues, and highly optimistic timelines were provided with no substantial change in representation over time. Scientists were the dominant voice with respect to translation timelines. The findings raise questions about the degree to which the media's overly optimistic slant fosters unrealistic expectations regarding the speed of clinical translation and highlight the ethical responsibility of stem cell researchers as public communicators. Copyright © 2015, American Association for the Advancement of Science.

  20. Portrayal of electronic cigarettes on YouTube.

    Science.gov (United States)

    Luo, Chuan; Zheng, Xiaolong; Zeng, Daniel Dajun; Leischow, Scott

    2014-10-03

    As the most popular video sharing website in the world, YouTube has the potential to reach and influence a huge audience. This study aims to gain a systematic understanding of what e-cigarette messages people are being exposed to on YouTube by assessing the quantity, portrayal and reach of e-cigarette videos. Researchers identified the top 20 search results on YouTube by relevance and view count for the following search terms: "electronic cigarettes", "e-cigarettes", "ecigarettes", "ecigs", "smoking electronic cigarettes", "smoking e-cigarettes", "smoking ecigarettes", "smoking ecigs". A sample of 196 unique videos was coded for overall portrayal and genre. Main topics covered in e-cigarette videos were recorded and video statistics and viewer demographic information were documented. Among the 196 unique videos, 94% (n = 185) were "pro" to e-cigarettes and 4% (n = 8) were neutral, while there were only 2% (n = 3) that were "anti" to e-cigarettes. The top 3 most prevalent genres of videos were advertisement, user sharing and product review. 84.3% of "pro" videos contained Web links for e-cigarette purchase. 71.4% of "pro" videos claimed that e-cigarettes were healthier than conventional cigarettes. Audience was primarily from the United States, the United Kingdom and Canada and "pro" e-cigarette videos were watched more frequently and rated much more favorably than "anti" ones. The vast majority of information on YouTube about e-cigarettes promoted their use and depicted the use of e-cigarettes as socially acceptable. It is critical to develop appropriate health campaigns to inform e-cigarette consumers of potential harms associated with e-cigarette use.

  1. amdSYM, a new dominant recyclable marker cassette for Saccharomyces cerevisiae

    NARCIS (Netherlands)

    Solis-Escalante, D.; Kuijpers, N.G.A.; Bongaerts, N.; Bolat, I.; Bosman, L.; Pronk, J.T.; Daran, J.M.; Daran-Lapujade, P.A.S.

    2012-01-01

    Despite the large collection of selectable marker genes available for Saccharomyces cerevisiae, marker availability can still present a hurdle when dozens of genetic manipulations are required. Recyclable markers, counterselectable cassettes that can be removed from the targeted genome after use,

  2. Association of ATP-binding cassette transporter-A1 polymorphism ...

    Indian Academy of Sciences (India)

    binding cassette transporter-A1 polymorphism with apolipoprotein AI level in Tehranian population. Sohrab Halalkhor Seyed Alireza Mesbah-Namin Maryam Sadat Daneshpour Mehdi Hedayati Fereidoun Azizi. Research Note Volume 90 Issue 1 ...

  3. 36 CFR 1280.44 - May I film, photograph, or videotape on NARA property for commercial purposes?

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 3 2010-07-01 2010-07-01 false May I film, photograph, or videotape on NARA property for commercial purposes? 1280.44 Section 1280.44 Parks, Forests, and Public... Rules for Filming, Photographing, or Videotaping on NARA Property? § 1280.44 May I film, photograph, or...

  4. Merging the Power of the Computer and VCR: Interactive Videotape with the Mandell Instant-Active Interface.

    Science.gov (United States)

    Brown, Scott W.

    1991-01-01

    Describes the Mandell Instant-Active Device, a microcomputer-based system that enables teachers to add questions and/or statement prompts to an existing videotape without affecting the videotape itself. Applications, procedures, student feedback, and hardware requirements are discussed. Ordering information is included. (MES)

  5. A Preliminary Study on the Thermal Performance of a Ventilated Honey Cassette for Stingless Bees

    Directory of Open Access Journals (Sweden)

    Basrawi Firdaus

    2017-01-01

    Full Text Available Stingless bees are very sensitive to the changes of surrounding temperature. A report stated that fertility rate in broodcell is 0% when the broodcell temperature is higher than 34°C or lower than 26°C. In addition, propolis made honey pot in a honey cassette also could melt when temperature is high. Therefore, the objective of this research is to investigate the temperature profile of a ventilated honey cassette exposed to outdoor conditions, and to evaluate the temperature regulation in the hive using the ventilated honey cassette. To achieve these objectives, two hives with conventional and ventilated honey cassettes were exposed under sun light in cloudy and sunny day. Temperature inside each hive was measured at 3 points and was compared. It was found that there is no significant different between the hives when both hives were exposed under direct sunlight in a cloudy day. However, two significant improvements were found for ventilated hive in sunny day. It could help to reduce temperature at wall of honey cassette consistently below 33°C. This could avoid the melting of propolis around the ventilated wall area. Furthermore, it could facilitate in better temperature reduction as compared to the conventional honey cassette. However, further study when there is a colony inside the hives must also be conducted to validate the results.

  6. Characterization of the novel In1059 harbouring VIM gene cassette

    Directory of Open Access Journals (Sweden)

    Dongguo Wang

    2017-05-01

    Full Text Available Abstract Background VIM-type enzyme encodes the most widely acquired metallo-β-lactamases in Gram- negative bacteria. To obtain current epidemiological data for integrons from enterobacteriae in hospital, the study characterizes the genetic structure in In1059 by comparison with In846 integrons harbouring VIM gene and other class 1 integrons including In37, In62, In843 and In1021 with the aim of identifying the putative mechanisms involved integron mobilization and infer evolution of relevant integrons. Methods Six of 69 recombinant plasmids from clinical strains were found to be class 1 integrons by digestion with BamHI, drug susceptibility testing, conjugation experiments, PCR amplification, integron cloning and sequencing, genome comparison, and detection of carbapenemase activity. Results The sequences of the six recombinant plasmids encoding In1021, In843, In846, In37, In62, and the novel In1059 integron had approximate lengths of ~4.8-, 4.1-, 5.1-, 5.3-, 5.3- and 6.6- kb, respectively. The genetic structures of these integrons were mapped and characterized, and the carbapenemase activities of their parental strains were assessed. Conclusions Our results suggest that the six variable integron structures and regular variations that exist in the gene cassettes provide a putative mechanism for the integron changes. Our study has also shown that the genetic features in the integrons named above fall within a scheme involving the stepwise and parallel evolution of class 1 integron variation likely under antibiotic selection pressure in clinical settings.

  7. Positive Portrayals of Old Age Do Not Always Have Positive Consequences.

    Science.gov (United States)

    Fung, Helene H; Li, Tianyuan; Zhang, Xin; Sit, Iny M I; Cheng, Sheung-Tak; Isaacowitz, Derek M

    2015-11-01

    The literature on "image of aging" suggests that exposure to positive portrayals of old age has positive downstream consequences for older adults. This study examined whether these positive consequences might have limits, such that they occurred for portrayals of old age that were positive, but not those that were extremely positive. Younger and older adults were allowed to selectively view (Study 1) or were experimentally exposed to (Studies 2 and 3) portrayals of old age of different levels of positivity. Their attention (Study 1) and physiological responses (Study 2) toward the portrayals, as well as perception of personal aging (Study 1) and memory performance (Study 3) after the exposure, were assessed. Findings from 3 studies suggested that older adults have a less negative perception of personal aging (Study 1) and a stronger calming physiological response (Study 2) when being exposed to portrayals of old age that were positive, but not extremely positive. Moreover, extremely positive portrayals lowered downstream memory performance (Study 3) and attracted less attention from older adults when they found these portrayals unrealistic (Study 1). These findings pinpoint the conditions under which positive portrayals of old age may benefit older adults. © The Author 2014. Published by Oxford University Press on behalf of The Gerontological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  8. A Theoretical Framework for Studying Media Portrayal of Persons with Disabilities.

    Science.gov (United States)

    Clogston, John S.

    Arguing that little research has been done on media portrayals of persons with disabilities, this paper reviews mass communication content studies of media portrayal of women and blacks in terms of deviance and minority group theories, and also reviews studies of media treatment of persons with disabilities from the rehabilitation and education…

  9. Portrayal of Depression and Other Mental Illnesses in Australian Nonfiction Media

    Science.gov (United States)

    Francis, Catherine; Pirkis, Jane; Blood, R. Warwick; Dunt, David; Burgess, Philip; Morley, Belinda; Stewart, Andrew

    2005-01-01

    This study describes Australian media portrayal of mental illnesses, focusing on depression. A random sample of 1,123 items was selected for analysis from a pool of 13,389 nonfictional media items about mental illness collected between March 2000 and February 2001. Depression was portrayed more frequently than other mental illnesses. Items about…

  10. Images with impact : The electoral consequences of party leader portrayal in the media

    NARCIS (Netherlands)

    Aaldering, L.

    2018-01-01

    This dissertation studies how the media portray party leaders in terms of their character traits, and when and to what extent these mediated leadership images have electoral consequences. The research focusses on the media portrayal of party leaders to establish leader effects, instead of leader

  11. Prime-Time Television Portrayals of Older Adults in the Context of Family Life.

    Science.gov (United States)

    Dail, Paula W.

    1988-01-01

    Content analyzed portrayal of older adults in 12 family-oriented, prime-time television programs to determine cognitive, physical, and health status; social interaction; and emotional behavior. Among 193 characters portraying elderly adults, 3,468 verbalizations and behaviors were examined. Results suggest that persons over age 55 are more…

  12. Analysis of the Image of Scientists Portrayed in the Lebanese National Science Textbooks

    Science.gov (United States)

    Yacoubian, Hagop A.; Al-Khatib, Layan; Mardirossian, Taline

    2017-01-01

    This article presents an analysis of how scientists are portrayed in the Lebanese national science textbooks. The purpose of this study was twofold. First, to develop a comprehensive analytical framework that can serve as a tool to analyze the image of scientists portrayed in educational resources. Second, to analyze the image of scientists…

  13. The Effect of Positive and Negative Movie Alcohol Portrayals on Transportation and Attitude Toward the Movie

    NARCIS (Netherlands)

    Koordeman, R.; Anschutz, D.J.; Engels, R.C.M.E.

    2014-01-01

    Background: This study examined the effects of alcohol portrayals on transportation and attitude toward a movie. In addition, we examined whether positive and negative movie alcohol portrayals affect transportation into and attitude toward the movie. Methods: A within-subject design was used in

  14. Today's Lesson by... Roosevelt? The Use of Character Portrayal in Classroom Guidance

    Science.gov (United States)

    Young, David S.

    2005-01-01

    This article describes how counselors may use the portrayal of a historical character in the classroom to teach lessons on self-esteem, motivation, and goal setting. Keeping with a creative spirit, a classroom guidance lesson that involves the use of character portrayal can be engaging for students. This involves the counselor assuming the…

  15. “Daughters of Eve”: Portrayal of the female body in selected HIV ...

    African Journals Online (AJOL)

    “Daughters of Eve”: Portrayal of the female body in selected HIV/AIDS songs in Malawi. Emmanuel Ngwira. Chancellor College, University of Malawi. & Stellenbosch University engwira@cc.ac.mw. Abstract. This paper examines how the female body is portrayed in selected. HIV/AIDS themed songs in Malawi. Generally, the ...

  16. Animal Classification. Animal Life in Action[TM]. Schlessinger Science Library. [Videotape].

    Science.gov (United States)

    2000

    This 23-minute videotape for grades 5-8, presents the myriad of animal life that exists on the planet. Students can view and perform experiments and investigations that help explain animal traits and habits. They learn what the terms "kingdom", "phylum", and "order" mean, and discover how the 3.5 million-plus organisms found on Earth fit into…

  17. Darwin's Revolution in Thought: An Illustrated Lecture. Teaching Guide and Videotape.

    Science.gov (United States)

    Gould, Stephen Jay

    "Darwin's Revolution in Thought" is Stephen Jay Gould's definitive treatise on Charles Darwin. This 50-minute classroom edition videotaped lecture is structured in the form of a paradox and three riddles about Darwin's life. Each is designed to shed light on one of the key features of the theory of natural selection, its philosophical…

  18. Marine & Other Invertebrates. Animal Life in Action[TM]. Schlessinger Science Library. [Videotape].

    Science.gov (United States)

    2000

    This 23-minute videotape for grades 5-8, presents the myriad of animal life that exists on the planet. Students can view and perform experiments and investigations that help explain animal traits and habits. Invertebrate animals include a vast array of spineless creatures. In this video, students discover marine lifeforms such as jellyfish,…

  19. Real Talk: Interviews, Radio Broadcasts and Videotaping as Context in Advanced Italian Conversation Courses.

    Science.gov (United States)

    Orban, Clara

    1997-01-01

    Discusses the problems faced by teachers of third-year (i.e. advanced) Italian language courses, particularly in developing students' speaking skills. Stresses the importance of "real talk" and discusses relevant student activities such as interviewing native speakers, preparing live radio broadcasts, and videotaping students speeches and…

  20. Amphibians. Animal Life in Action[TM]. Schlessinger Science Library. [Videotape].

    Science.gov (United States)

    2000

    This 23-minute videotape for grades 5-8, presents the myriad of animal life that exists on the planet. Students can view and perform experiments and investigations that help explain animal traits and habits. Students find out about the world of amphibians as they examine their physical characteristics, environments, and life cycles, as well as…

  1. Camera Perspective Bias in Videotaped Confessions: Experimental Evidence of Its Perceptual Basis

    Science.gov (United States)

    Ratcliff, Jennifer J.; Lassiter, G. Daniel; Schmidt, Heather C.; Snyder, Celeste J.

    2006-01-01

    The camera perspective from which a criminal confession is videotaped influences later assessments of its voluntariness and the suspect's guilt. Previous research has suggested that this camera perspective bias is rooted in perceptual rather than conceptual processes, but these data are strictly correlational. In 3 experiments, the authors…

  2. Robert Sylwester on Social Interaction and Brain Development. Windows to the Mind, Volume 1. [Videotape].

    Science.gov (United States)

    Sylwester, Robert

    This videotape explores the relationship between the child's social world and cognitive development. The first part of the video examines why the social environment is important to brain development. This section looks at the amount of time a child spends in a social environment, and the anatomy of the developing brain. The second part of the…

  3. Insects & Other Arthropods. Animal Life in Action[TM]. Schlessinger Science Library. [Videotape].

    Science.gov (United States)

    2000

    This 23-minute videotape for grades 5-8, presents the myriad of animal life that exists on the planet. Students can view and perform experiments and investigations that help explain animal traits and habits. They also learn that there are more species of insects than any other animal class in the world. Insects are incredible creatures with many…

  4. The Importance of Child Development in Education: A Conservation with James Comer and Chip Wood. [Videotape].

    Science.gov (United States)

    Comer, James; Wood, Chip

    Taped before an audience of teachers from around the country, this 65-minute videotape presents a discussion between James Comer and Chip Wood, noted experts in child development and education, in which they converse and respond to questions about critical issues confronting educators today. During the first part of the video, Comer and Wood…

  5. Dancetime! 500 Years of Social Dance. Volume I: 15th-19th Centuries. [Videotape].

    Science.gov (United States)

    Teten, Carol

    This VHS videotape recording is the first in a two-volume series that presents 500 years of social dance, music, and fashion. It focuses on the 15th-19th centuries, including Renaissance nobility, Baroque extravagance, Regency refinement, and Victorian romanticism. Each era reflects the changing relationships between men and women through the…

  6. Dancetime! 500 Years of Social Dance. Volume II: 20th Century. [Videotape].

    Science.gov (United States)

    Teten, Carol

    This 50-minute VHS videotape is the second in a 2-volume series that presents 500 years of social dance, music, and fashion. It features dance and music of the 20th century, including; 1910s: animal dances, castle walk, apache, and tango; 1920s: black bottom and charleston; 1930s: marathon, movie musicals, big apple, and jitterbug; 1940s: rumba;…

  7. Video-Taped Instruction Creates Listening and Visual Memory Integration for Higher Reading and Math Scores.

    Science.gov (United States)

    Erland, Jan

    This pre-post quasi-experimental study was conducted in a public school 5th grade class to determine the effects of video-taped instruction in teaching analysis and pattern finding skills. Methodology included guidelines from Cognitive Behavior Modification, Suggestopedia, and Guilford's Structure of Intellect Model within the Kaufman and Kaufman…

  8. Birds. Animal Life in Action[TM]. Schlessinger Science Library. [Videotape].

    Science.gov (United States)

    2000

    This 23-minute videotape for grades 5-8, presents the myriad of animal life that exists on the planet. Students can view and perform experiments and investigations that help explain animal traits and habits. There are many different types of birds, over 9,000 different species. While not all birds take to the air, they all have feathers. Students…

  9. A system for sampling changes in blood pressure from a videotape, using Finapres(TM)

    NARCIS (Netherlands)

    Näring, G.W.B.; Wittebrood, J.; Staak, C. van der; DeMey, H.; Schaap, C.

    1996-01-01

    A system is described that measures blood pressure noninvasively and continuously during a videotaped verbal interaction. The system incorporates the use of the Finapres to nonintrusively and continuously measure BP during a verbal interaction. Segments from the interaction, in which blood pressure

  10. How To Dance through Time. Volume II: Dances of the Ragtime Era, 1910-1920. [Videotape].

    Science.gov (United States)

    Teten, Carol

    This 59-minute VHS videotape is the second in a series of "How To Dance Through Time" videos. It provides 44 step combinations and how-to instructions to help viewers learn to dance the most popular dances of the early 20th century (the ragtime era), including: the wild animal dances (fox trot, horse trot, kangaroo hop, duck waddle, squirrel,…

  11. Presenting Social Issues with Videotape [and] Teaming Up to Take a Risk.

    Science.gov (United States)

    Agosta, Diana; Jackson, Dick

    1991-01-01

    Two articles discuss the use of media in schools. One describes the use of videotapes to present social issues; the second describes the use of an integrated learning system with ninth and tenth grade at-risk students to improve their rate of attendance, academic achievement, and self-esteem. (LRW)

  12. Reptiles. Animal Life in Action[TM]. Schlessinger Science Library. [Videotape].

    Science.gov (United States)

    1999

    This 23-minute videotape for grades 5-8, presents the myriad of animal life that exists on the planet. Students can view and perform experiments and investigations that help explain animal traits and habits. The ancestors of reptiles date back to the dinosaurs. After the dinosaurs died out, it was one of the best-adapted species that survived and…

  13. A Videotape Series for Teaching Physicians to Evaluate Sexually Abused Children

    Science.gov (United States)

    Jones, Jerry G.; Garrett, Judy; Worthington, Toss

    2004-01-01

    A free videotape subscription series was utilized to increase the knowledge of general physicians in clinical practice about the medical evaluation of sexually abused children. Of the 65 physicians who requested the first tape, 39 (60%) completed it. Fourteen of the 39 physicians who completed the first tape (36%) completed the 5-tape series.…

  14. Food Chains. Animal Life in Action[TM]. Schlessinger Science Library. [Videotape].

    Science.gov (United States)

    2000

    This 23-minute videotape for grades 5-8, presents the myriad of animal life that exists on the planet. Students can view and perform experiments and investigations that help explain animal traits and habits. The food chain provides a clear example of how life continues year after year. Students learn how the cycle of energy starts with the sun,…

  15. Trends in explicit portrayal of suicidal behavior in popular U.S. movies, 1950-2006.

    Science.gov (United States)

    Jamieson, Patrick Edwin; Romer, Dan

    2011-01-01

    Trends in suicidal behavior portrayal in movies may reflect greater societal acceptance of suicide with potential adverse effects on adolescents. To assess the potential for such adverse effects, explicit portrayals of suicidal behavior and the ratings of films were coded in top-grossing U.S. movies from 1950-2006 (N = 855). Suicidal behavior portrayal in films increased linearly from 1950 to 2006. From 1968-1984, movies rated R by the Motion Picture Association of America had 5 times more highly explicit suicide behavior portrayals than did G/PG films. After the adoption of the PG-13 category in 1985, PG-13 and R films were indistinguishable on this measure. The results indicate the need for further study of the effects of suicidal behavior portrayals on adolescent movie audiences.

  16. The portrayal of single women characters in selected African literary texts

    Directory of Open Access Journals (Sweden)

    Linda Kwatsha

    2015-03-01

    Full Text Available In this article, the portrayal of single women characters in works by a number of African women authors is critically reflected upon. These women are portrayed as strong, resistant, independent and realistic characters, who boldly resist male paternalism and dominance in order to look to the future with courage. Single women characters are presented as independent individuals rather than as kinship appendages. They are portrayed as architects of their own potential happiness rather than as passive receivers of the dictates of patriarchy. The article will also show that women characters account for their own values, rather than subserviently surrender to stereotypical conventions. African women writers portray single women characters as having the professional and economic means to look after themselves. This portrayal of women characters is part of a debate that translates itself to contemporary everyday philosophical social theory.

  17. The effect of videotaping students' interviews with patients for interview skill education.

    Science.gov (United States)

    Lee, Woo Sung; Hwang, Ji Young; Lim, Ji Eun; Suh, Sang-Yeon; Park, Ki Heum; Sung, Nak-Jin

    2013-03-01

    The importance of communication between patients and physicians has been proven in many previous studies. The authors analyzed the effect of interview skill education through videotapes which recorded students' interviews with real patients in the outpatient department of family medicine. This study was conducted with all students who chose the elective course of family medicine and one randomly selected student every week from an 'infectious internal medicine' class at Dongguk University Ilsan Hospital during the period from December 2008 to March 2011. All students performed a preliminary examination of a new patient at the outpatient department of family medicine. All consultations were videotaped. Feedback to the student was given on the same day by viewing the videotape together. After feedback, all students performed another preliminary examination of one new patient at the department of family medicine the same week. Three family medicine residents scored all videotapes using 10-item interview skill checklists. Many parts of the checklists were modified using the Arizona Clinical Interview Rating Scales. Thirty-three students participated. Of 10 items, nine showed increased scores after feedback. There was a significant change in four items after feedback: 'type of question' (before 2.36 ± 0.60, after 2.73 ± 0.72), 'timeline' (before 2.82 ± 0.68, after 3.18 ± 0.73), 'positive verbal reinforcement' (before 2.24 ± 0.56, after 2.61 ± 0.90), and the total score (before 21.70 ± 2.62, after 23.39 ± 3.13) (P skills using videotapes of students' preliminary consultations with real patients in outpatient settings, was effective in improving the interview areas of 'type of question,' 'timeline,' 'positive verbal reinforcement,' and the total interview scores.

  18. Pc promoter from class 2 integrons and the cassette transcription pattern it evokes.

    Science.gov (United States)

    da Fonseca, Érica Lourenço; dos Santos Freitas, Fernanda; Vicente, Ana Carolina Paulo

    2011-04-01

    Integrons are considered expression systems due to the presence of Pc promoters that drive gene cassette transcription. The role and configurations of Pc are well known in class 1 integrons; however, this region has not yet been identified in class 2 integrons. This study aimed to characterize the Pc promoter from class 2 integrons and to determine the effect of gene cassette position on transcription driven by this promoter. The class 2 cassette arrays from Klebsiella pneumoniae and Vibrio cholerae strains were determined by sequencing. Transcription analyses were performed by real-time RT-PCR and relative quantification was carried out by comparing the transcripts of each normalized gene inserted in the integron to each other. The resistance profile was determined by the disc diffusion method. The class 2 Pc promoter was characterized by 5' rapid amplification of cDNA ends and promoter prediction programs. Sequence analysis revealed the presence of the dfrA1-sat2-aadA1-ybeA and sat2-aadA1-ybeA arrangements in K. pneumoniae and V. cholerae strains, respectively. Real-time RT-PCR showed that the transcription of the first cassettes was higher than that of distal ones in wild-type and recombinant strains. All strains were resistant, indicating cassette expression. The Pc promoter of class 2 integrons (-35 TTTAAT |16 bp| -10 TAAAAT) was determined based on in silico analyses and on the transcription start site sequence of the class 2 integron cassette array. The Pc from class 2 integrons was characterized for the first time and the cassette position effect on transcription was demonstrated.

  19. Ground Testing of the EMCS Seed Cassette for Biocompatibility with the Tardigrade, Hypsibius dujardini

    Science.gov (United States)

    Reinsch, Sigrid; Myers, Zachary Alan; DeSimone, Julia Carol; Freeman, John L.; Steele, Marianne K.; Sun, Gwo-Shing; Heathcote, David

    2014-01-01

    The European Modular Cultivation System, EMCS, was developed by ESA for plant experiments. We performed ground testing to determine whether ARC EMCS seed cassettes could be adapted for use with tardigrades for future spaceflight experiments. Tardigrades (water bears) are small invertebrates that enter the tun state in response to desiccation or other environmental stresses. Tardigrade tuns have suspended metabolism and have been shown to be survive exposure to space vacuum, high pressure, temperature and other stresses. For spaceflight experiments using the EMCS, the organisms ideally must be able to survive desiccation and storage in the cassette at ambient temperature for several weeks prior to the initiation of the experiment by the infusion of water to the cassette during spaceflight. The ability of tardigrades to survive extremes by entering the tun state make them ideal candidates for growth experiments in the EMCS cassettes. The growth substratum in the cassettes is a gridded polyether sulfone (PES) membrane. A blotter beneath the PES membrane contains dried growth medium. The goals of our study were to (1) determine whether tardigrades survive and reproduce on PES membranes, (2) develop a consistent method for dehydration of the tardigrades with high recovery rates upon rehydration, (3) to determine an appropriate food source for the tardigrades that can also be dehydrated/rehydrated and (4) successful mock rehydration experiment in cassettes with appropriate food source. We present results that show successful multigenerational growth of tardigrades on PES membranes with a variety of wet food sources. We have successfully performed a mock rehydration with tardigrades and at least one candidate food, protonema of the moss Polytrichum, that supports multigenerational growth and whose spores germinate quickly enough to match tardigrade feeding patterns post rehydration. Our results indicate that experiments on the ISS using the tardigrade, Hypsibius dujardini

  20. ATP-binding cassette transporters in reproduction: a new frontier

    Science.gov (United States)

    Bloise, E.; Ortiga-Carvalho, T.M.; Reis, F.M.; Lye, S.J.; Gibb, W.; Matthews, S.G.

    2016-01-01

    BACKGROUND The transmembrane ATP-binding cassette (ABC) transporters actively efflux an array of clinically relevant compounds across biological barriers, and modulate biodistribution of many physiological and pharmacological factors. To date, over 48 ABC transporters have been identified and shown to be directly and indirectly involved in peri-implantation events and fetal/placental development. They efflux cholesterol, steroid hormones, vitamins, cytokines, chemokines, prostaglandins, diverse xenobiotics and environmental toxins, playing a critical role in regulating drug disposition, immunological responses and lipid trafficking, as well as preventing fetal accumulation of drugs and environmental toxins. METHODS This review examines ABC transporters as important mediators of placental barrier functions and key reproductive processes. Expression, localization and function of all identified ABC transporters were systematically reviewed using PubMed and Google Scholar websites to identify relevant studies examining ABC transporters in reproductive tissues in physiological and pathophysiological states. Only reports written in English were incorporated with no restriction on year of publication. While a major focus has been placed on the human, extensive evidence from animal studies is utilized to describe current understanding of the regulation and function of ABC transporters relevant to human reproduction. RESULTS ABC transporters are modulators of steroidogenesis, fertilization, implantation, nutrient transport and immunological responses, and function as ‘gatekeepers’ at various barrier sites (i.e. blood-testes barrier and placenta) against potentially harmful xenobiotic factors, including drugs and environmental toxins. These roles appear to be species dependent and change as a function of gestation and development. The best-described ABC transporters in reproductive tissues (primarily in the placenta) are the multidrug transporters p-glycoprotein and

  1. Ethnicity, genetics, and breast cancer: media portrayal of disease identities.

    Science.gov (United States)

    Donelle, L; Hoffman-Goetz, L; Clarke, J N

    2005-08-01

    To describe, compare, and analyze how the risk of breast cancer is framed in newspapers directed towards an ethnic minority population (Jewish) with higher risk of inherited breast cancer compared with newspaper coverage for the general population (Anglo-Canadian) without this risk. This investigation utilized a mixed methods (quantitative and qualitative) approach. The design emphasized a content analysis conducted on ethnically specific and non-ethnic newspaper articles. It is noteworthy that the 'Jewish' newspapers devote a substantially larger proportion of articles on breast cancer to genetic risk as the key risk factor for this disease. Articles in the Jewish newspapers tend to link being a Jewish woman with being at risk for a diagnosis of breast cancer. This ethnic 'identity' is reinforced through the repeated association of Jewish heritage and genetic breast cancer risk at the exclusion of other known risk factors. This isolated genetic link to breast cancer is not a message that is replicated within the provincial newsprint articles. These findings assist in the facilitation of prevention and treatment of those with or at risk of breast cancer. The health policy implications of this portrayal as well as suggestions for change are considered.

  2. Being female: the portrayal of women's health in print media.

    Science.gov (United States)

    Shugg, Jo; Liamputtong, Pranee

    2002-01-01

    In this article, we examine the portrayal of women's health issues in two of Melbourne's print media: The Age and the Herald Sun. In particular, we aim to examine the patterns of coverage of women's health and the underlying message in articles on women's health issues. We employed an unobtrusive method, and the study was conducted over a three-month period: May to July 1998. We found that many of the articles and public reactions centred on women and reproduction. This implies that women are still seen in relation to their reproductive role in Australian society. In addition, there are many issues regarding women and their health missing from the media's publication during the research period. The message emerging from the analysis implies that if women do not fit into the "ideal women" framework of the society, their health needs are given little attention by the print media and, therefore, their health is likely to suffer. This can be interpreted as one form of women's oppression in Australian society.

  3. Gender-Role Portrayals in Television Advertising Across the Globe.

    Science.gov (United States)

    Matthes, Jörg; Prieler, Michael; Adam, Karoline

    Although there are numerous studies on gender-role portrayals in television advertising, comparative designs are clearly lacking. With content analytical data from a total of 13 Asian, American, and European countries, we study the stereotypical depiction of men and women in television advertisements. Our sample consists of 1755 ads collected in May 2014. Analyzing the gender of the primary character and voiceover, as well as the age, associated product categories, home- or work setting, and the working role of the primary character, we concluded that gender stereotypes in TV advertising can be found around the world. A multilevel model further showed that gender stereotypes were independent of a country's gender indices, including Hofstede's Masculinity Index, GLOBE's Gender Egalitarianism Index, the Gender-related Development Index, the Gender Inequality Index, and the Global Gender Gap Index. These findings suggest that gender stereotyping in television advertising does not depend on the gender equality prevalent in a country. The role of a specific culture in shaping gender stereotypes in television advertising is thus smaller than commonly thought.

  4. Portrayal of organ donation and transplantation on American primetime television.

    Science.gov (United States)

    Harbaugh, Calista; Afana, Majed; Burdick, Stephanie; East, Joseph; Kodali, Sindhura; Lee, Jay; Patel, Shaun; Rangrass, Govind; Ranney, David; Sood, Vikram; Lynch, Raymond; Sonnenday, Christopher J; Englesbe, Michael J; Mathur, Amit K

    2011-01-01

    Recently, both living and deceased organ donation rates have hit a plateau, despite increases in need for viable organs. One approach to improve donation rate is public education and policy; thus, it is necessary to understand the information the public is receiving regarding organ donation. We hypothesized that primetime medical dramas portray organ donation and transplantation in a negative manner. We compiled data on all primetime medical drama episodes with transplant themes from November 2008 through June 2010 and assessed depictions of organ donors and transplant candidates. Positive and negative thematic elements surrounding the process and individuals involved were also identified. One hundred and fifty-five million and 145 million households watched episodes containing any negative message and any positive message, respectively. Episodes containing only negative messages had over twice the household viewership per episode compared to episodes containing only positive messages (8.4 million vs. 4.1 million, p = 0.01). Widespread exposure to these representations may reinforce public misconceptions of transplantation. The transplant community should consider the popularity of medical dramas as an opportunity to impact the perception of organ donation and transplantation for millions of Americans. © 2011 John Wiley & Sons A/S.

  5. The portrayal of Tourette Syndrome in film and television.

    Science.gov (United States)

    Calder-Sprackman, Samantha; Sutherland, Stephanie; Doja, Asif

    2014-03-01

    To determine the representation of Tourette Syndrome (TS) in fictional movies and television programs by investigating recurrent themes and depictions. Television and film can be a source of information and misinformation about medical disorders. Tourette Syndrome has received attention in the popular media, but no studies have been done on the accuracy of the depiction of the disorder. International internet movie databases were searched using the terms "Tourette's", "Tourette's Syndrome", and "tics" to generate all movies, shorts, and television programs featuring a character or scene with TS or a person imitating TS. Using a grounded theory approach, we identified the types of characters, tics, and co-morbidities depicted as well as the overall representation of TS. Thirty-seven television programs and films were reviewed dating from 1976 to 2010. Fictional movies and television shows gave overall misrepresentations of TS. Coprolalia was overrepresented as a tic manifestation, characters were depicted having autism spectrum disorder symptoms rather than TS, and physicians were portrayed as unsympathetic and only focusing on medical therapies. School and family relationships were frequently depicted as being negatively impacted by TS, leading to poor quality of life. Film and television are easily accessible resources for patients and the public that may influence their beliefs about TS. Physicians should be aware that TS is often inaccurately represented in television programs and film and acknowledge misrepresentations in order to counsel patients accordingly.

  6. [The scientific videotape with digital processing in surgery. The new opportunities offered surgery for videotape recording and postprocessing with the use of information and digital technologies].

    Science.gov (United States)

    Picardi, N

    1999-01-01

    The facility of the tape recording of a surgical operation, by means of simple manageable apparatuses and at low costs, especially in comparison with the former cinematography, makes it possible for all surgeons to record their own operative activity. Therefore at present the demonstration in video of surgical interventions is very common, but very often the video-tapes show surgical events only in straight chronological succession, as for facts of chronicle news. The simplification of the otherwise sophisticated digital technology of informatics elaboration of images makes more convenient and advisable to assemble the more meaningful sequences for a final product of higher scientific value. The digital technology gives at the best its contribution during the phase of post-production of the video-tape, where the surgeon himself can assemble an end product of more value because aimed to a scientific and rational communication. Thanks to such an elaboration the video-tape can aim not simply to become a good documentary, but also to achieve an educational purpose or becomes a truly scientific film. The initial video will be recorded following a specific project, the script, foreseeing and programming what has to be demonstrated of the surgical operation, establishing therefore in advance the most important steps of the intervention. The sequences recorded will then be assembled not necessarily in a chronological succession but integrating the moving images with static pictures, as drawings, schemes, tables, aside the picture-in picture technique, and besides the vocal descriptive comment. The cinema language has accustomed us to a series of passages among the different sequences as fading, cross-over, "flash-back", aiming to stimulate the psychological associative powers and encourage those critical. The video-tape can be opportunely shortened, paying attention to show only the essential phases of the operation for demonstrate only the core of the problem and utilize

  7. Effect of attC structure on cassette excision by integron integrases

    Directory of Open Access Journals (Sweden)

    Larouche André

    2011-02-01

    Full Text Available Abstract Background Integrons are genetic elements able to integrate and disseminate genes as cassettes by a site-specific recombination mechanism. These elements contain a gene coding for an integrase that carries out recombination by interacting with two different target sites; the attI site in cis with the integrase and the palindromic attC site of a gene cassette. Integron integrases (IntIs bind specifically to the bottom strand of attC sites. The extrahelical bases resulting from folding of attC bottom strands are important for the recognition by integrases. These enzymes are directly involved in the accumulation and formation of new cassette arrangements in the variable region of integrons. Thus, it is important to better understand interactions between IntIs and their substrates. Results We compared the ability of five IntIs to carry out excision of several cassettes flanked by different attC sites. The results showed that for most cassettes, IntI1 was the most active integrase. However, IntI2*179E and SonIntIA could easily excise cassettes containing the attCdfrA1 site located upstream, whereas IntI1 and IntI3 had only a weak excision activity for these cassettes. Analysis of the secondary structure adopted by the bottom strand of attCdfrA1 has shown that the identity of the extrahelical bases and the distance between them (A-N7-8-C differ from those of attCs contained in the cassettes most easily excisable by IntI1 (T-N6-G. We used the attCdfrA1 site upstream of the sat2 gene cassette as a template and varied the identity and spacing between the extrahelical bases in order to determine how these modifications influence the ability of IntI1, IntI2*179E, IntI3 and SonIntIA to excise cassettes. Our results show that IntI1 is more efficient in cassette excision using T-N6-G or T-N6-C attCs while IntI3 recognizes only a limited range of attCs. IntI2*179E and SonIntIA are more tolerant of changes to the identity and spacing of extrahelical

  8. Cassettes for PCR-mediated gene tagging in Candida albicans utilizing nourseothricin resistance.

    Science.gov (United States)

    Milne, Stephen W; Cheetham, Jill; Lloyd, Deborah; Aves, Stephen; Bates, Steven

    2011-12-01

    In recent years a number of molecular tools have been reported for use in the human fungal pathogen Candida albicans, including PCR-mediated approaches for gene disruption, conditional expression and epitope tagging. Traditionally these methods have utilized auxotrophic markers; however, the availability of auxotrophic markers can be limiting and in some instances their use may also impact on the interpretation of results. As a result, the use of positive selection markers has now become more commonplace. Here we report the development and validation of a set of cassettes for PCR-mediated gene tagging and overexpression studies utilizing the nourseothricin resistance (CaNAT1) positive selection marker. In particular we have produced cassettes containing yeast-enhanced GFP, YFP, CFP, RFP and a combined V5-6xHis epitope tag. The cassettes are engineered for use in PCR-mediated gene tagging strategies where insertion is targeted to the 3' end of the gene of interest. In addition, to facilitate protein functional analysis and genetic suppression studies through the use of overexpression, we have also constructed a promoter replacement cassette containing the ENO1 promoter which is known to be expressed at a high level. These cassettes expand on the range of molecular tools available for working with C. albicans and may also be used in other Candida species that display sensitivity to nourseothricin. Copyright © 2011 John Wiley & Sons, Ltd.

  9. Fundamentals of Laparoscopic Surgery manual test: is videotaped performance assessment an option?

    Science.gov (United States)

    Rooney, Deborah M; Brissman, Inga C; Finks, Jonathan F; Gauger, Paul G

    2015-01-01

    In efforts to maintain standards required to evaluate the high-stakes assessment, Society of American Gastrointestinal and Endoscopic Surgeons Fundamentals of Laparoscopic Surgery (FLS) requires all new proctors to complete the train-the-proctor workshop. As the pool of FLS proctors expands, new methods to streamline training and quality assurance programs should be considered. We propose that videotaped performances of the FLS manual tasks may be an alternative proxy to live assessment for training of new proctors, but evaluation of proctors' measures from videotaped FLS performances is required before implementation. A 2-phased research consisted of capturing newly trained proctors' (n = 20) ratings of 3 similar FLS performances across 3 stations-live (Live), videotaped-laparoscopic only (Lap Only) view, and videotaped-dual (Dual) views, during the 2012 Society of American Gastrointestinal and Endoscopic Surgeons FLS train-the-proctor workshop. A month later, a sample of proctors (n = 9) viewed videotaped versions of live FLS performances originally observed during the workshop. Captured metrics include recognition of a predefined critical error for each task (dichotomously scored and summed) and time to complete each of the 5 tasks. Analysis of variance compared the proctors' summed ratings for similar performances across Live, Lap Only, and Dual views, whereas paired t test compared recorded times of Lap Only vs Dual views, Live vs web ratings, and proctors' recorded times across the Lap Only and Dual views. There were neither differences in ratings across Live, Lap Only, and Dual views (p = 0.49) nor in recorded times for performances viewed across Lap Only and Dual viewing options (p = 0.29 and 0.76, respectively). Mean summed performance ratings observed live (4.6) were higher than those observed via the web (4.0), although not significant (p = 0.051). There were no differences in recorded times for identical performances across Live and web observations (p

  10. The Ideal of Human Body and its Portrayal in the Media

    OpenAIRE

    Vaněk, Miroslav

    2011-01-01

    The ideal of the human body and it's portrayal in the media The bachelor thesis named "The ideal of the human body and it's portrayal in the media" compares the differences between the approach to the human body in the Holy scripture and modern media, how the media presents it, especially in women's magazines. This also points to more serious problems, which can be caused by the portrayal of the human body presented in modern media. Key words: Ideal, human body, person, media, the beauty, the...

  11. The portrayal of bone tumours in the press.

    Science.gov (United States)

    Al-Nammari, Shafic Said; Danesh, Arash; Mussa, Mohamed; Al-Hadithy, Nawfal

    2013-04-01

    The media are pivotal in educating and informing the general public. The stories they cover and how they cover them has a powerful influence on public perceptions. There have been no previous reports of the portrayal of bone tumours in the press. LexisNexis™ Professional search engine used to retrieve articles from all United Kingdom National Newspapers over one year containing terms "bone tumour/bone tumour" and 46 other named bone and joint tumours from May 2009 to May 2010. A total of 253 relevant articles were found. Seven per cent solely bone tumour related, 41% main theme and 52% mentioned in passing. 52% mentioned tumour type. These were 51% multiple myeloma, 15% Ewing's sarcoma, 9% sarcoma unspecified, 6% clear cell sarcoma, 4.5% epithelial sarcoma, 4% synovial sarcoma, 3% osteosarcoma, 3% bony metastasis and 1.5% chondrosarcoma. Benign bone tumours not mentioned. Article focus: chemotherapy 17%, surgeon/doctor 7% and new surgical technique 2%. The overall attitudinal tone of the articles were 52% negative, 32% neutral and 16% positive. Only 13% quoted an oncologist, and 1% an orthopaedic surgeon. Quality of medical information provided was limited with 90% providing no meaningful medical information and this medical information being correct only 68% of the time. Articles with quotes from a doctor were significantly more likely to contain meaningful medical information than those without-33 versus 4%, respectively (p < 0.001 Chi-squared test)-and there was a trend for them to be more factually correct overall-68 versus 50% (p = 0.192 Fisher's exact Test).

  12. Childhood videotaped social and neuromotor precursors of schizophrenia: a prospective investigation

    DEFF Research Database (Denmark)

    Schiffman, Jason; Walker, Elaine; Ekstrøm, Morten

    2004-01-01

    were filmed under standardized conditions while they were eating lunch. The examination was part of a larger study investigating early signs of schizophrenia spectrum disorders. Many of the subjects had a parent with schizophrenia, leaving them at high risk for developing a schizophrenia spectrum...... disorder and children who developed other psychiatric disorders. RESULTS: The findings from this study suggest that the brief videotaped footage of children eating lunch was able to discriminate between the individuals who later developed schizophrenia and those who did not. Specifically...... disorder. In 1991, adult psychiatric outcome data were obtained for 91.3% (N=242). This study systematically analyzed the videotapes to determine whether the children who developed schizophrenia as adults evidenced greater social and/or neuromotor deficits than children who did not develop a psychiatric...

  13. Pain, role play, and videotape. Pain management staff development in a community hospital.

    Science.gov (United States)

    Daroszewski, E B; Meehan, D A

    1997-01-01

    Videotaped role play was an effective staff development strategy used as an initial exercise in a five-part class to update the pain management skills of experienced nurses. It engaged the participants in learning and stimulated discussion and provided concrete feedback of current clinical practices for comparison with the Agency for Health Care Policy and Research pain management guidelines (Acute Pain Management Guideline Panel, 1992).

  14. Image of the Nigeria police as portrayed in Georgie Iortema's Night ...

    African Journals Online (AJOL)

    Image of the Nigeria police as portrayed in Georgie Iortema's Night Duty. ... Creative Artist: A Journal of Theatre and Media Studies ... agents caught fighting, demanding and accepting bribe, killing indiscriminately, and raping women, etc.

  15. Ideology of Post-Feminism: Portrayal through Visual Advertisements in India

    National Research Council Canada - National Science Library

    D Gowrisankar; I Ajit

    2016-01-01

    .... The post-feminist 'equality portrayals' of women are visible in cinema, electronic and mass media advertisements and also in literature in the form of avoidance from depicting a young woman as passive, inferior, weaker and subordinate to a man.

  16. Portrayal of childhood cancer in English language magazines in North America: 1970-2001.

    Science.gov (United States)

    Clarke, Juanne

    2005-01-01

    This article is a content and discourse analysis of the portrayal of childhood cancer in English language magazines in North America. In a unique specification of published research on the media portrayal of disease, magazines were divided into three market or audience groupings called (1) science, (2) news/special interest, and (3) other (women/teen/parenting/health). The predominate frames or discoursesin these three groups were compared and differences were found amongst them and discussed in the article. Considerable evidence suggests that people with cancer are stigmatized. In the analyzed media focused on children, those with cancer are highly idealized and stereotyped. On the one hand, this can be thought of as a very positive portrayal of children in this situation. Children are described as if they possess heroic and idealized character traits, appearances, social characteristics, and personalities. Possible links between this idealized, polarized, and biased portrayal of children with cancer and their documented experiences of stigma are discussed.

  17. Serum albumin promotes ATP-binding cassette transporter-dependent sterol uptake in yeast

    DEFF Research Database (Denmark)

    Marek, Magdalena; Silvestro, Daniele; Fredslund, Maria D.

    2014-01-01

    Sterol uptake in fungi is a multistep process that involves interaction between external sterols and the cell wall, incorporation of sterol molecules into the plasma membrane, and subsequent integration into intracellular membranes for turnover. ATP-binding cassette (ABC) transporters have been....... cerevisiae requires the presence of serum or albumin for efficient cholesterol uptake. These results suggest that albumin can serve as sterol donor in ABC transporter-dependent sterol uptake, a process potentially important for growth of C. glabrata inside infected humans. Serum albumin can serve as sterol...... donor in ATP-binding cassette-transporter-dependent sterol uptake, a process potentially important for growth of Candida glabrata inside infected humans....

  18. How Media Portrayal Affects Perceptions of Minorities : The case of Bulgaria

    OpenAIRE

    Georgieva, Radostina

    2013-01-01

    A connection is often drawn between the way the media represent ethnic minorities and the way majorities perceive them. This paper seeks to investigate further this link by drawing parallels between media portrayal of ethnic minorities in Bulgaria and the majority's attitude towards them. In addition it introduces the results of an online survey in which 250 people took part that directly studies the effects of negative media portrayal on the subject's attitude towards ethnic minorities in Bu...

  19. Older people's portrayal in the print media: implications for intergenerational relations

    OpenAIRE

    Roos, Vera; Sedick, Samiera

    2011-01-01

    This study explored the perceptions of older people in a residential care facility regarding how they are portrayed in the print media and the implications of such portrayal for intergenerational relations. Twenty-one older residents in a residential care facility participated voluntarily in the study (men = 9, women = 12; age range 60 to 85 years). Data were collected using exploratory interviews and focus groups followed by the thematic analysis of the data. The findings suggest that the ol...

  20. The Oppressions On Women As Portrayed In Beryonce’s Selected Songs

    OpenAIRE

    Kris, Indri Hariyanti

    2016-01-01

    This thesis entitled The Oppressions On Women As Portrayed In Beyonce Selected Songs. In those selected songs, Beyonce portrays the social phenomenon of the women who often get bad treatment of social and family. This thesis covers the analysis of oppressions or the pressures experienced by women either physically or emotionally. This thesis also covers the analysis of the solutions that Beyonce given to the oppressions that are experienced by women. The conclusion of this thes...

  1. The impact of cosmetic surgery media portrayals on body image and attitudes

    OpenAIRE

    Ashikali, Eleni-Marina

    2014-01-01

    The cosmetic surgery industry has rapidly expanded and Professional Associations for surgery in the UK and USA have expressed concern over the ways in which surgery is portrayed in the media. This thesis aimed to investigate how different portrayals of cosmetic surgery in the media impact women and adolescent girls’ body image and attitudes towards surgery. Moreover, it examined a number of moderating variables which may affect responses to such media.\\ud The first three studies examined the ...

  2. Visual portrayals of obesity in health media: promoting exercise without perpetuating weight bias.

    Science.gov (United States)

    Pearl, R L; Dovidio, J F; Puhl, R M

    2015-08-01

    Health education campaigns for preventing and reducing obesity often contain weight-stigmatizing visual content, which may have unintended negative health consequences. The goal of the present research was to identify non-stigmatizing visual content for health education materials that can promote exercise among people of diverse weight statuses. An online sample of 483 US women viewed: (i) a woman with obesity portrayed stereotypically; (ii) a woman with obesity exercising; (iii) a woman with obesity portrayed neutrally; or (iv) a lean woman exercising. Race of the models pictured was randomized (White or Black). Participants completed measures of weight bias and exercise behavior and attitudes, and provided information about their weight status. Analysis of covariance revealed that responses to stereotypical and exercise images varied by participant weight status. Across participants, neutral obesity portrayals elicited lower expressions of weight-biased attitudes and higher reports of exercise liking/comfort. Among non-overweight participants, images portraying women with obesity stereotypically or counter-stereotypically produced greater endorsement of negative stereotypes than control, lean images. No effects of model race were found. These findings suggest that the public responds differently to visual portrayals of obesity depending on weight status, and neutral portrayals may be an effective route toward promoting exercise without perpetuating stigma. © The Author 2015. Published by Oxford University Press. All rights reserved. For permissions, please email: journals.permissions@oup.com.

  3. Portrayals of men and women in Drum magazine (South Africa advertisements

    Directory of Open Access Journals (Sweden)

    Mlenga Jere

    2014-02-01

    Full Text Available Orientation: The subject of gender portrayals in advertising continues to generate academicdiscussions in part because of its socialisation effects.Research purpose: The purpose of this study is to determine how print advertisements inDrum magazine portray women and men based on a number of categories including traitdescriptors, physical characteristics, role behaviours and occupational status.Motivation for the study: It is important to understand gender portrayals in advertisingbecause perceptions of social reality are influenced by what people are exposed to insocialisation agents such as advertisements.Research approach, design and method: A quantitative content analysis was run on a sampleof 415 advertisements drawn from Drum magazine. Partial least squares analysis was used toassess the relationships between the variables.Main findings: The findings show that gender has a statistically significant effect on profiles(roles and sexual appeal but not on gender equity. Advertisers therefore treat modelsdifferently with regard to the roles assigned and the use of sexual appeal.Practical/managerial implications: Previous research evidence and theory indicate that thecontent of advertisements does influence readers’ self-perception and also their perceptionof others. Within the bounds of advertising creativity, it is important that advertisers payparticular attention to gender portrayals in advertising to avoid the creation or perpetuationof gender stereotypes.Contribution/value-add: Though the model’s gender influences portrayals in advertising,other contextual factors are also important determinants of portrayals.

  4. Provider portrayals and patient-provider communication in drama and reality medical entertainment television shows.

    Science.gov (United States)

    Jain, Parul; Slater, Michael D

    2013-01-01

    Portrayals of physicians on medical dramas have been the subject of research attention. However, such research has not examined portrayals of interactions between physicians and patients, has not compared physician portrayals on medical dramas versus on medical reality programs, and has not fully examined portrayals of physicians who are members of minority groups or who received their education internationally. This study content-analyzes 101 episodes (85 hours) of such programs broadcast during the 2006-2007 viewing season. Findings indicate that women are underrepresented as physicians on reality shows, though they are no longer underrepresented as physicians on dramas. However, they are not as actively portrayed in patient-care interactions as are male physicians on medical dramas. Asians and international medical graduates are underrepresented relative to their proportion in the U.S. physician population, the latter by almost a factor of 5. Many (but certainly not all) aspects of patient-centered communication are modeled, more so on reality programs than on medical dramas. Differences in patient-provider communication portrayals by minority status and gender are reported. Implications for public perception of physicians and expectations regarding provider-patient interaction are discussed.

  5. Novel streptomycin and spectinomycin resistance gene as a gene cassette within a class 1 integron isolated from Escherichia coli

    DEFF Research Database (Denmark)

    Sandvang, D.

    1999-01-01

    The aadA genes, encoding resistance to streptomycin and spectinomycin, have been found as gene cassettes in different gram-negative and gram-positive bacterial species. The present study has revealed the sequence of a new gene, aadA5, integrated as a gene cassette together with the trimethoprim...

  6. Fictions of Work-Related Learning: How a Hit Television Show Portrays Internship, and How Medical Students Relate to Those Portrayals

    Science.gov (United States)

    Jubas, Kaela; Knutson, Patricia

    2013-01-01

    This article proceeds from three main premises. First, we assert that popular culture functions pedagogically and helps cultural consumers learn about work, even before they enter educational programs or workplaces. Second, we argue that exploring portrayals of internship is useful in understanding the "attributes of formality and informality"…

  7. Development of a modified gentamicin resistance cassette for genetic manipulation of the oral spirochete Treponema denticola.

    Science.gov (United States)

    Bian, Jiang; Fenno, J Christopher; Li, Chunhao

    2012-03-01

    Herein, we report that a modified gentamicin cassette and a PCR-based method can be used for targeted mutagenesis of the oral spirochete Treponema denticola. This approach minimizes polar effects and spontaneous antibiotic resistance. Therefore, it can serve as a reliable tool for genetic manipulation of T. denticola.

  8. Engineered XcmI cassette-containing vector for PCR-based ...

    Indian Academy of Sciences (India)

    T-vector; direct cloning; XcmI cassette; sequencing; PCR; marine population genetics. Author Affiliations. Futoshi Aranishi1 2 Takane Okimoto1 3. Molecular Biology Division, National Institute of Fisheries Science, Yokohama 236-8648, Japan; Department of Biological and Environmental Sciences, Mirjazaki University, ...

  9. Simultaneous acquisition of storage phosphor and asymmetric screen-film chest images using a hybrid cassette

    Science.gov (United States)

    Stewart, Brent K.; Kimme-Smith, Carolyn; Johnson, Sandra L.; Johnson, Timothy; Aberle, Denise R.

    1994-05-01

    A hybrid cassette has been developed for simultaneous acquisition of storage phosphor and asymmetric screen-film chest images. This is important for the collection of images for Receiver Operating Characteristic studies comparing conventional radiography and computed radiography, without either increased exposure or non-identical imaging conditions. This hybrid radiographic cassette consists of a computed radiography imaging plate (in front) and an intact, high contrast variant of a commercially available asymmetric screen-film system (in the rear) with a speed of approximately 425. The high contrast, speed and efficiency of this screen-film system allow for positioning of the storage phosphor plate in the front of the cassette. As the imaging plate absorption is approximately 35%, the fast screen-film system provides high quality diagnostic images. There is minimal beam hardening, which is ameliorated by the high contrast of the asymmetric front screen. There is minimal differences in the Plexiglas step wedge phantom gray level values for CR and CR-hybrid images and in optical density values for InSightTM and InSightTM-hybrid films. The signal to noise ratio of either hybrid image, while fractionally less than their standard counterparts, is negligibly so. Only a slight modification in radiographic technique is required (10%) for use of this hybrid cassette, providing images that are virtually the same as those obtained through the standard CR and InSightTM ITC imaging methods.

  10. A fluorescent cassette-based strategy for engineering multiple domain fusion proteins

    Directory of Open Access Journals (Sweden)

    Khorchid Ahmad

    2003-07-01

    Full Text Available Abstract Background The engineering of fusion proteins has become increasingly important and most recently has formed the basis of many biosensors, protein purification systems, and classes of new drugs. Currently, most fusion proteins consist of three or fewer domains, however, more sophisticated designs could easily involve three or more domains. Using traditional subcloning strategies, this requires micromanagement of restriction enzymes sites that results in complex workaround solutions, if any at all. Results Therefore, to aid in the efficient construction of fusion proteins involving multiple domains, we have created a new expression vector that allows us to rapidly generate a library of cassettes. Cassettes have a standard vector structure based on four specific restriction endonuclease sites and using a subtle property of blunt or compatible cohesive end restriction enzymes, they can be fused in any order and number of times. Furthermore, the insertion of PCR products into our expression vector or the recombination of cassettes can be dramatically simplified by screening for the presence or absence of fluorescence. Conclusions Finally, the utility of this new strategy was demonstrated by the creation of basic cassettes for protein targeting to subcellular organelles and for protein purification using multiple affinity tags.

  11. ATP-binding cassette (ABC) transporters in normal and pathological lung

    NARCIS (Netherlands)

    van der Deen, M; de Vries, EGE; Timens, W; Scheper, RJ; Timmer-Bosscha, H; Postma, DS

    2005-01-01

    ATP-binding cassette ( ABC) transporters are a family of transmembrane proteins that can transport a wide variety of substrates across biological membranes in an energy-dependent manner. Many ABC transporters such as P-glycoprotein ( P-gp), multidrug resistance-associated protein 1 ( MRP1) and

  12. Identification of a novel streptococcal gene cassette mediating SOS mutagenesis in Streptococcus uberis

    NARCIS (Netherlands)

    Varhimo, Emilia; Savijoki, Kirsi; Jalava, Jari; Kuipers, Oscar P.; Varmanen, Pekka

    Streptococci have been considered to lack the classical SOS response, defined by increased mutation after UV exposure and regulation by LexA. Here we report the identification of a potential self-regulated SOS mutagenesis gene cassette in the Streptococcaceae family. Exposure to UV light was found

  13. Trinucleotide cassettes increase diversity of T7 phage-displayed peptide library

    Directory of Open Access Journals (Sweden)

    McMahon James B

    2007-10-01

    Full Text Available Abstract Background Amino acid sequence diversity is introduced into a phage-displayed peptide library by randomizing library oligonucleotide DNA. We recently evaluated the diversity of peptide libraries displayed on T7 lytic phage and M13 filamentous phage and showed that T7 phage can display a more diverse amino acid sequence repertoire due to differing processes of viral morphogenesis. Methods In this study, we evaluated and compared the diversity of a 12-mer T7 phage-displayed peptide library randomized using codon-corrected trinucleotide cassettes with a T7 and an M13 12-mer phage-displayed peptide library constructed using the degenerate codon randomization method. Results We herein demonstrate that the combination of trinucleotide cassette amino acid codon randomization and T7 phage display construction methods resulted in a significant enhancement to the functional diversity of a 12-mer peptide library. This novel library exhibited superior amino acid uniformity and order-of-magnitude increases in amino acid sequence diversity as compared to degenerate codon randomized peptide libraries. Comparative analyses of the biophysical characteristics of the 12-mer peptide libraries revealed the trinucleotide cassette-randomized library to be a unique resource. Conclusion The combination of T7 phage display and trinucleotide cassette randomization resulted in a novel resource for the potential isolation of binding peptides for new and previously studied molecular targets.

  14. Metabolism of ATP-binding cassette drug transporter inhibitors: complicating factor for multidrug resistance

    NARCIS (Netherlands)

    Cnubben, N.H.P.; Wortelboer, H.M.; Zanden, van J.J.; Rietjens, I.M.C.M.; Bladeren, van P.J.

    2005-01-01

    Membrane transport proteins belonging to the ATP-binding cassette (ABC) family of transport proteins play a central role in the defence of organisms against toxic compounds, including anticancer drugs. However, for compounds that are designed to display a toxic effect, this defence system diminishes

  15. Metabolism of ATP-binding cassette drug transporter inhibitors: complicating factor for multidrug resistance.

    NARCIS (Netherlands)

    Cnubben, N.H.; Wortelboer, H.M.; Zanden, J.J. van; Rietjens, I.M.; Bladeren, P.J. van

    2005-01-01

    Membrane transport proteins belonging to the ATP-binding cassette (ABC) family of transport proteins play a central role in the defence of organisms against toxic compounds, including anticancer drugs. However, for compounds that are designed to display a toxic effect, this defence system diminishes

  16. Functional Analysis of Detergent‐Solubilized and Membrane‐Reconstituted ATP‐Binding Cassette Transporters

    NARCIS (Netherlands)

    Poolman, Bert; Doeven, Mark K.; Geertsma, Eric R.; Biemans‐Oldehinkel, Esther; Konings, Wil N.; Rees, Douglas C.

    2005-01-01

    ATP‐binding cassette (ABC) transporters are vital to any living system and are involved in the translocation of a wide variety of substances, from ions and nutrients to high molecular weight proteins. This chapter describes methods used to purify and membrane reconstitute ABC transporters in a fully

  17. Keys: Career Exploration. Cassettes and Films, Career Guidance and the Kuder Interest Inventories, and Guide.

    Science.gov (United States)

    Lombard, Jack; Grinager, Marilyn

    This set of 17 related items, intended for Grades 6-9, consists of 10 filmstrips, 5 cassettes, and a manual. The first filmstrip explains the Kuder E. interest profile and gives an overview of the filmstrip series, and the remaining filmstrips are divided along the 10 Kuder E. General Interest Survey categories. However, the filmstrips and…

  18. ATimer-Actuated, Immunoassay Cassette for Detecting Molecular Markers in Oral Fluids

    Science.gov (United States)

    Liu, Changchun; Qiu, Xianbo; Ongagna, Serge; Chen, Dafeng; Chen, Zongyuan; Abrams, William R.; Malamud, Daniel; Corstjens, Paul L.A.M.; Bau, Haim H.

    2009-01-01

    An inexpensive, hand-held, point-of-care, disposable, self-contained, immunoassay cassette comprised of air pouches for pumping, a metering chamber, reagents storage chambers, a mixer, and a lateral flow strip was designed, constructed, and tested. The assay was carried out in a consecutive flow format. The detection was facilitated with up-converting, phosphor (UCP) reporter particles. The automated, timely pumping of the various reagents was driven by a spring-loaded timer. The utility of the cassette was demonstrated by detecting antibodies to HIV in saliva samples and further evaluated with a non-contagious, haptenized DNA assay. The cassette has several advantages over dip sticks such as sample preprocessing, integrated storage of reagents, and automated operation that reduces operator errors and training. The cassette and actuator described herein can readily be extended to detect biomarkers of other diseases in body fluids and other fluids at the point of care. The system is particularly suitable for resource poor countries, where funds and trained personnel are in short supply. PMID:19255658

  19. A novel thin NIPAM gel cassette dosimeter for photon-beam radiotherapy.

    Directory of Open Access Journals (Sweden)

    Ling-Ling Hsieh

    Full Text Available The response of thin polymer gel cassettes (called NIPAM gels to ionizing radiation was investigated in this study. The NIPAM gels were prepared from gelatin, N-isopropyl acrylamide, tetrakis (hydroxymethyl phosphoniumchloride, and N,N'-methylene-bis-acrylamide. Gel cassettes were irradiated in a phantom using a linear accelerator, and the polymerization morphology of irradiated NIPAM gel was characterized using scanning electron microscopy. The dose-response sensitivity of the NIPAM gels was evaluated using the differences in optical densities. The optical densities were obtained using a computer-controlled CCD camera that was connected to a planar illumination source for acquisition of optical transmission images. The central axis depth dose profiles of the phantom were extracted, and a comparison with ionization chamber measurements demonstrated similarities in profiles. The sensitivity, linearity of the response, accuracy, and reproducibility of the polymer gel cassettes were acceptable. However, the profiles of the half-blocked field irradiation showed no significant dispersion in the visible region. This study also extensively investigated the spatial stability of the NIPAM gel. The results showed that the gel cassette response remains stable for up to three months after irradiation.

  20. Association of ATP-binding cassette transporter-A1 polymorphism ...

    Indian Academy of Sciences (India)

    environment might be modified in a more personalized fash- ion to help prevent or delay ... Journal of Genetics, Vol. 90, No. 1, April 2011 ..... aged children. Metabolism 53, 182–186. Yin K., Liao D. F. and Tang C. K. 2010 ATP-binding mem- brane cassette transporter A1 (ABCA1): a possible link between inflammation and ...

  1. Utilizing ARC EMCS Seedling Cassettes as Highly Versatile Miniature Growth Chambers for Model Organism Experiments

    Science.gov (United States)

    Freeman, John L.; Steele, Marianne K.; Sun, Gwo-Shing; Heathcote, David; Reinsch, S.; DeSimone, Julia C.; Myers, Zachary A.

    2014-01-01

    The aim of our ground testing was to demonstrate the capability of safely putting specific model organisms into dehydrated stasis, and to later rehydrate and successfully grow them inside flight proven ARC EMCS seedling cassettes. The ARC EMCS seedling cassettes were originally developed to support seedling growth during space flight. The seeds are attached to a solid substrate, launched dry, and then rehydrated in a small volume of media on orbit to initiate the experiment. We hypothesized that the same seedling cassettes should be capable of acting as culture chambers for a wide range of organisms with minimal or no modification. The ability to safely preserve live organisms in a dehydrated state allows for on orbit experiments to be conducted at the best time for crew operations and more importantly provides a tightly controlled physiologically relevant growth experiment with specific environmental parameters. Thus, we performed a series of ground tests that involved growing the organisms, preparing them for dehydration on gridded Polyether Sulfone (PES) membranes, dry storage at ambient temperatures for varying periods of time, followed by rehydration. Inside the culture cassettes, the PES membranes were mounted above blotters containing dehydrated growth media. These were mounted on stainless steel bases and sealed with plastic covers that have permeable membrane covered ports for gas exchange. The results showed we were able to demonstrate acceptable normal growth of C.elegans (nematodes), E.coli (bacteria), S.cerevisiae (yeast), Polytrichum (moss) spores and protonemata, C.thalictroides (fern), D.discoideum (amoeba), and H.dujardini (tardigrades). All organisms showed acceptable growth and rehydration in both petri dishes and culture cassettes initially, and after various time lengths of dehydration. At the end of on orbit ISS European Modular Cultivation System experiments the cassettes could be frozen at ultra-low temperatures, refrigerated, or chemically

  2. Ground Testing of the EMCS Seed Cassette for Biocompatibility with the Cellular Slime Mold, Dictyostelium Discoideum

    Science.gov (United States)

    Hanely, Julia C.; Reinsch, Sigrid; Myers, Zachary A.; Freeman, John; Steele, Marianne K.; Sun, Gwo-Shing; Heathcote, David G.

    2014-01-01

    The European Modular Cultivation System, EMCS, was developed by ESA for plant experiments. To expand the use of flight verified hardware for various model organisms, we performed ground experiments to determine whether ARC EMCS Seed Cassettes could be adapted for use with cellular slime mold for future space flight experiments. Dictyostelium is a cellular slime mold that can exist both as a single-celled independent organism and as a part of a multicellular colony which functions as a unit (pseudoplasmodium). Under certain stress conditions, individual amoebae will aggregate to form multicellular structures. Developmental pathways are very similar to those found in Eukaryotic organisms, making this a uniquely interesting organism for use in genetic studies. Dictyostelium has been used as a genetic model organism for prior space flight experiments. Due to the formation of spores that are resistant to unfavorable conditions such as desiccation, Dictyostelium is also a good candidate for use in the EMCS Seed Cassettes. The growth substratum in the cassettes is a gridded polyether sulfone (PES) membrane. A blotter beneath the PES membranes contains dried growth medium. The goals of this study were to (1) verify that Dictyostelium are capable of normal growth and development on PES membranes, (2) develop a method for dehydration of Dictyostelium spores with successful recovery and development after rehydration, and (3) successful mock rehydration experiments in cassettes. Our results show normal developmental progression in two strains of Dictyostelium discoideum on PES membranes with a bacterial food source. We have successfully performed a mock rehydration of spores with developmental progression from aggregation to slug formation, and production of morphologically normal spores within 9 days of rehydration. Our results indicate that experiments on the ISS using the slime mold, Dictyostelium discoideum could potentially be performed in the flight verified hardware of

  3. Headless, hungry, and unhealthy: a video content analysis of obese persons portrayed in online news.

    Science.gov (United States)

    Puhl, Rebecca M; Peterson, Jamie Lee; DePierre, Jenny A; Luedicke, Joerg

    2013-01-01

    The news media has substantial influence on public perceptions of social and health issues. This study conducted a video content analysis to examine portrayals of obese persons in online news reports about obesity. The authors downloaded online news videos about obesity (N = 371) from 5 major news websites and systematically coded visual portrayals of obese and nonobese adults and youth in these videos. The authors found that 65% of overweight/obese adults and 77% of overweight/obese youth were portrayed in a negative, stigmatizing manner across multiple obesity-related topics covered in online news videos. In particular, overweight/obese individuals were significantly more likely than were nonoverweight individuals to be portrayed as headless, with an unflattering emphasis on isolated body parts, from an unflattering rear view of their excess weight, eating unhealthy foods, engaging in sedentary behavior, and dressed in inappropriately fitting clothing. Nonoverweight individuals were significantly more likely to be portrayed positively. In conclusion, obese children and adults are frequently stigmatized in online news videos about obesity. These findings have important implications for public perceptions of obesity and obese persons and may reinforce negative societal weight bias.

  4. Mental health on screen: A DSM-5 dissection of portrayals of autism spectrum disorders in film and TV

    OpenAIRE

    Nordahl-Hansen, Anders Johan; Tøndevold, Magnus; Fletcher-Watson, Sue

    2017-01-01

    Portrayals of characters with autism spectrum disorders (ASD) in films and TV series are subject to intense debate over whether such representations are accurate. Inaccurate portrayals are a concern as they may lead to increased stereotypes toward the condition. We investigate whether portrayals of characters with autism spectrum disorder in film and TV-series align with DSM-5 diagnostic criteria. Our data show that characters present a full range of characteristics described in the DSM-5. Th...

  5. Portrayal of Women’s Images in Television Dramas: A Malaysian Case

    Directory of Open Access Journals (Sweden)

    Ibrahim Faridah

    2017-01-01

    Full Text Available Current situations brought about by globalisation and sophisticated innovations in new technology not only have created a new understanding of the world around us but also conscientiously sensitised society, particularly the women towards a more active role in shaping their world. An important contributory factor that can determine the success of women is their centrality in the media, through media positions held or through media images portrayed. What this paper has endeavoured to show is the portrayal of women in the media particularly, television dramas. In today’s scenario, the role of women keeps changing, so does the portrayal of women’s images in television dramas. This paper, based on a qualitative content analysis study of five popular Malaysian television dramas, found that the stereotypical generalizations of women’s images are still existing but to a lesser degree.

  6. Watching each other: portrayals of gender and ethnicity in television advertisements.

    Science.gov (United States)

    Rubie-Davies, Christine M; Liu, Sabrina; Lee, Kai-Chi Katie

    2013-01-01

    The stereotypical depiction of men and women and of ethnic minority groups in advertisements is problematic because studies have shown that repeated exposure to selective portrayals of particular groups can lead to viewers adopting distorted beliefs about those groups. The current study examined the contemporary portrayal of men and women and of ethnic minority groups in New Zealand television advertisements. Over 3,000 advertisements were coded. Men and women were less often depicted in stereotypical roles than has previously been reported. However, White actors dominated the screen with Maori and Pacific Island people only being proportionally represented in advertisements that presented negative stereotyping. The findings were similar for Asians whose portrayal also appeared to be stereotypical. The authors call for more equitable appearances of women and ethnic minority groups.

  7. The integrated nature of the method of psychological portraying in law enforcement

    Directory of Open Access Journals (Sweden)

    Shpagina E. M.

    2016-01-01

    Full Text Available The article reveals peculiarities of using the method of psychological portraying in law enforcement, shows the current practice of using psychological knowledge in solving crimes, reveals an integration relationship of other Sciences with psychology with the use of the method of psychological portraying the disclosure of crimes. The article presents a comprehensive approach to the psychological profile of an unknown offender. Psychological portraying of an unknown offender is regarded as a form of analytical work in solving crimes, which integrate information and knowledge from various Sciences such as forensics, criminology, detective work, psychiatry, sexology, victimology, etc. Psychological science (information of its various branches is a fundamental element that helps to explain, interpret and describe the behavior of the offender and the victim to resolve the problems of law enforcement.

  8. Quantification of video-taped images in microcirculation research using inexpensive imaging software (Adobe Photoshop).

    Science.gov (United States)

    Brunner, J; Krummenauer, F; Lehr, H A

    2000-04-01

    Study end-points in microcirculation research are usually video-taped images rather than numeric computer print-outs. Analysis of these video-taped images for the quantification of microcirculatory parameters usually requires computer-based image analysis systems. Most software programs for image analysis are custom-made, expensive, and limited in their applicability to selected parameters and study end-points. We demonstrate herein that an inexpensive, commercially available computer software (Adobe Photoshop), run on a Macintosh G3 computer with inbuilt graphic capture board provides versatile, easy to use tools for the quantification of digitized video images. Using images obtained by intravital fluorescence microscopy from the pre- and postischemic muscle microcirculation in the skinfold chamber model in hamsters, Photoshop allows simple and rapid quantification (i) of microvessel diameters, (ii) of the functional capillary density and (iii) of postischemic leakage of FITC-labeled high molecular weight dextran from postcapillary venules. We present evidence of the technical accuracy of the software tools and of a high degree of interobserver reliability. Inexpensive commercially available imaging programs (i.e., Adobe Photoshop) provide versatile tools for image analysis with a wide range of potential applications in microcirculation research.

  9. The passionate 'sharing' of creative women : A Study of self-portrayal on Facebook and Instagram

    OpenAIRE

    Aerni, Marianne

    2014-01-01

    Online Self-portrayal has been attracting attention since the rise of social networks and their integration into everyday life. Social media have been said to support the idea of an “endlessly constructed self,” transporting culture and shaping people’s online experiences. Research often focused on the if and why when mostly college students portrayed themselves on social networks and in online communities. The aim of this study is to deepen the understanding of how a certain demographic of w...

  10. A Content Analysis of the Roles Portrayed by Women in Commercials: 1973 - 2008

    Directory of Open Access Journals (Sweden)

    Claudia Rosa Acevedo

    2010-12-01

    Full Text Available The principal objective of this paper was to examine female roles portrayed in advertising. More specifically, the question that stimulated this research project was: What message has been signaled to society through advertisements about women? Have these portrayals altered throughout the past decades? The study consisted of a systematic content analysis of Brazilian commercials between 1973 and 2008. A probabilistic sample procedure was adopted. 95 pieces of material were selected. Our results have revealed that certain specific images have changed over the years; however, they continue to be stereotyped and idealized. DOI: 10.5585/remark.v9i3.2201

  11. An exploration on possible correlations among perception and physical characteristics of EMOVO emotional portrayals

    Directory of Open Access Journals (Sweden)

    Carlo Giovannella

    2013-02-01

    Full Text Available This article presents the first attempt to investigate the existence of possible quantitative correlations among the physical characteristics of emotional portrayals and the emotions perceived by humans during their listening. Our aim was: a to design and develop a new investigation procedure/protocol; b to obtain information useful to recognition and synthesis of emotions conveyed by the human voice. Our results, obtained on a subset of the emotional portrayals contained in the corpus EMOVO, show that, apart from well known qualitative indications, it is also possible to observe clear quantitative trends for some couples of 'emotionsignal characteristics' as function of the recognition rate of the emotion.

  12. Rare codons effect on expression of recombinant gene cassette in Escherichia coli BL21(DE3

    Directory of Open Access Journals (Sweden)

    Aghil Esmaeili-Bandboni

    2017-11-01

    Full Text Available Objective: To demonstrate the sensitivity of expression of fusion genes to existence of a large number of rare codons in recombinant gene sequenced. Methods: Primers for amplification of cholera toxin B, Shiga toxin B and gfp genes were designed by Primer3 software and synthesized. All of these 3 genes were cloned. Then the genes were fused together by restriction sites and enzymatic method. Two linkers were used as a flexible bridge in connection of these genes. Results: Cloning and fusion of cholera toxin B, Shiga toxin B and gfp genes were done correctly. After that, expression of the recombinant gene construction was surveyed. Conclusions: According to what was seen, because of the accumulation of 12 rare codons of Shiga toxin B and 19 rare codons of cholera toxin B in this gene cassette, the expression of the recombinant gene cassette, in Escherichia coli BL21, failed.

  13. Concept design of DEMO divertor cassette remote handling: Simply supported beam approach

    Energy Technology Data Exchange (ETDEWEB)

    Mozzillo, Rocco [CREATE, University of Naples Federico II, DII, P.le Tecchio 80, 80125, Naples (Italy); Di Gironimo, Giuseppei, E-mail: peppe.digironimo@gmail.com [CREATE, University of Naples Federico II, DII, P.le Tecchio 80, 80125, Naples (Italy); Mäkinen, Harri [VTT Technical Research Centre of Finland, P.O. Box 1300, FI-33101 Tampere (Finland); Miccichè, Gioacchino [ENEA – CR Brasimone, I-40032 Camugnano, BO (Italy); Määttä, Timo [VTT Technical Research Centre of Finland, P.O. Box 1300, FI-33101 Tampere (Finland)

    2017-03-15

    Highlights: • The present work focused on a new approach to the design of DEMO Divertor Cassette Remote Handling Equipment. • The work provides an alternative approach to the design based on the concept of a simply supported beam. • The approach proposed focuses a Divertor Cassette mover that performs the maintenance of the three cassettes at each port. • First rough dimensioning of the main components has been provided and demonstrating the feasibility of the design solutions. • The main idea of the work consisted on a design capable to use knowledge already adopted in industrial contexts. - Abstract: The present work focused on the development of a new approach to the concept design of DEMO Divertor Cassette (DC) Remote Handling Equipment (RHE). The approach is based on three main assumptions: the DC remote handling activities and the equipment shall be simplified as much as possible; technologies well known and consolidated in the industrial context can be adopted also in the nuclear fusion field; the design of the RHE should be based on a simply supported beam approach instead of cantilever approach. In detail, during the maintenance activities the barycentre of the DC is centred with respect to DC supports. This solution could simplify the design of RHE with a consequent reduction of the design and development costs. Moreover also the DC remote handling tasks shall be simplified in order to better manage the DC maintenance processes. For this reason the DC assembly and disassembly process has been simplified dividing the main sequences in basic movements. For each movement a dedicated tool has been conceived.

  14. A novel multi-purpose cassette for repeated integrative epitope tagging of genes in Saccharomyces cerevisiae.

    Science.gov (United States)

    De Antoni, A; Gallwitz, D

    2000-04-04

    Gene tagging can be achieved by homologous recombination in yeast. The kan(r) marker gene plays an important role in PCR-mediated gene disruption and PCR-mediated epitope tagging experiments. In this paper, new modules containing a tag-loxP-kanMX-loxP cassette are described that allow tagging of different genes by using the kan(r) marker repeatedly.

  15. Functional Analysis of Detergent‐Solubilized and Membrane‐Reconstituted ATP‐Binding Cassette Transporters

    OpenAIRE

    Poolman, Bert; Doeven, Mark K.; Geertsma, Eric R.; Biemans‐Oldehinkel, Esther; Konings, Wil N.; Rees, Douglas C.

    2005-01-01

    ATP‐binding cassette (ABC) transporters are vital to any living system and are involved in the translocation of a wide variety of substances, from ions and nutrients to high molecular weight proteins. This chapter describes methods used to purify and membrane reconstitute ABC transporters in a fully functional state. The procedures are largely based on our experience with substrate‐binding protein‐dependent ABC uptake systems from bacteria, but the approaches should be applicable to multisubu...

  16. Energy transfer cassettes based on organic fluorophores: construction and applications in ratiometric sensing.

    Science.gov (United States)

    Fan, Jiangli; Hu, Mingming; Zhan, Peng; Peng, Xiaojun

    2013-01-07

    This tutorial review presents some recent developments in the construction and applications of cassettes based on resonance energy transfer between fluorescent dyes in the visible and infrared region. We focused on the contributions of different connections between the energy donor and acceptor according to the "through-space" and "through-bond" methods, and emphasised their applications in ratiometric sensing for the detection of ions and small molecules.

  17. Identification and Characterization of an ATP Binding Cassette l-Carnitine Transporter in Listeria monocytogenes

    OpenAIRE

    Fraser, Katy R.; Harvie, Duncan; Coote, Peter J.; O'Byrne, Conor P.

    2000-01-01

    We identified an operon in Listeria monocytogenes EGD with high levels of sequence similarity to the operons encoding the OpuC and OpuB compatible solute transporters from Bacillus subtilis, which are members of the ATP binding cassette (ABC) substrate binding protein-dependent transporter superfamily. The operon, designated opuC, consists of four genes which are predicted to encode an ATP binding protein (OpuCA), an extracellular substrate binding protein (OpuCC), and two membrane-associated...

  18. Conservation of gene cassettes among diverse viruses of the human gut.

    Directory of Open Access Journals (Sweden)

    Samuel Minot

    Full Text Available Viruses are a crucial component of the human microbiome, but large population sizes, high sequence diversity, and high frequencies of novel genes have hindered genomic analysis by high-throughput sequencing. Here we investigate approaches to metagenomic assembly to probe genome structure in a sample of 5.6 Gb of gut viral DNA sequence from six individuals. Tests showed that a new pipeline based on DeBruijn graph assembly yielded longer contigs that were able to recruit more reads than the equivalent non-optimized, single-pass approach. To characterize gene content, the database of viral RefSeq proteins was compared to the assembled viral contigs, generating a bipartite graph with functional cassettes linking together viral contigs, which revealed a high degree of connectivity between diverse genomes involving multiple genes of the same functional class. In a second step, open reading frames were grouped by their co-occurrence on contigs in a database-independent manner, revealing conserved cassettes of co-oriented ORFs. These methods reveal that free-living bacteriophages, while usually dissimilar at the nucleotide level, often have significant similarity at the level of encoded amino acid motifs, gene order, and gene orientation. These findings thus connect contemporary metagenomic analysis with classical studies of bacteriophage genomic cassettes. Software is available at https://sourceforge.net/projects/optitdba/.

  19. Optimized invertase expression and secretion cassette for improving Yarrowia lipolytica growth on sucrose for industrial applications.

    Science.gov (United States)

    Lazar, Zbigniew; Rossignol, Tristan; Verbeke, Jonathan; Crutz-Le Coq, Anne-Marie; Nicaud, Jean-Marc; Robak, Małgorzata

    2013-11-01

    Yarrowia lipolytica requires the expression of a heterologous invertase to grow on a sucrose-based substrate. This work reports the construction of an optimized invertase expression cassette composed of Saccharomyces cerevisiae Suc2p secretion signal sequence followed by the SUC2 sequence and under the control of the strong Y. lipolytica pTEF promoter. This new construction allows a fast and optimal cleavage of sucrose into glucose and fructose and allows cells to reach the maximum growth rate. Contrary to pre-existing constructions, the expression of SUC2 is not sensitive to medium composition in this context. The strain JMY2593, expressing this new cassette with an optimized secretion signal sequence and a strong promoter, produces 4,519 U/l of extracellular invertase in bioreactor experiments compared to 597 U/l in a strain expressing the former invertase construction. The expression of this cassette strongly improved production of invertase and is suitable for simultaneously high production level of citric acid from sucrose-based media.

  20. Extending the Glucosyl Ceramide Cassette Approach: Application in the Total Synthesis of Ganglioside GalNAc-GM1b

    Directory of Open Access Journals (Sweden)

    Miku Konishi

    2013-12-01

    Full Text Available The development of a novel cyclic glucosyl ceramide cassette acceptor for efficient glycolipid syntheses was investigated. p-Methoxybenzyl (PMB groups were selected as protecting groups at C2 and C3 of the glucose residue with the aim of improving the functionality of the cassette acceptor. The choice of the PMB group resulted in a loss of β-selectivity, which was corrected by using an appropriate tether to control the spatial arrangement and the nitrile solvent effect. To investigate the effect of linker structure on the β-selectivity of intramolecular glycosylation, several linkers for tethering the glucose and ceramide moiety were designed and prepared, namely, succinyl, glutaryl, dimethylmalonyl, and phthaloyl esters. The succinyl ester linker was the best for accessing the cassette form. The newly designed glucosyl ceramide cassette acceptor was then applied in the total synthesis of ganglioside GalNAc-GM1b.

  1. Enhanced performance of methamphetamine lateral flow cassettes using an electronic lateral flow reader.

    Science.gov (United States)

    Smith, Jerome P; Sammons, Deborah L; Robertson, Shirley A; Snawder, John E

    2015-01-01

    Surface contamination from methamphetamine in meth labs continues to be a problem. We had previously developed a lateral flow assay cassette for field detection of methamphetamine contamination that is commercially available and has been used by a number of groups to assess contamination. This cassette uses the complete disappearance of the test line as an end point for detection of 50 ng/100 cm2 of methamphetamine contamination for surface sampling with cotton swabs. In the present study, we further evaluate the response of the cassettes using an electronic lateral flow reader to measure the intensities of the test and control lines. The cassettes were capable of detecting 0.25 ng/ml for calibration solutions. For 100 cm2 ceramic tiles that were spiked with methamphetamine and wiped with cotton-tipped wooden swabs wetted in assay/sampling buffer, 1 ng/tile was detected using the reader. Semi-quantitative results can be produced over the range 0-10 ng/ml for calibration solutions and 0-25 ng/tile for spiked tiles using either a 4-parameter logistic fit of test line intensity versus concentration or spiked mass or the ratio of the control line to the test line intensity fit to concentration or spiked mass. Recovery from the tiles was determined to be about 30% using the fitted curves. Comparison of the control line to the test line was also examined as a possible visual detection end point and it was found that the control line became more intense than the test line at 0.5 to 1 ng/ml for calibration solutions or 1 to 2 ng/tile for spiked tiles. Thus the lateral flow cassettes for methamphetamine have the potential to produce more sensitive semi-quantitative results if an electronic lateral flow reader is used and can be more sensitive for detection if the comparison of the control line to the test line is used as the visual end point.

  2. There's alcohol in my soap: portrayal and effects of alcohol use in a popular television series.

    Science.gov (United States)

    van Hoof, Joris J; de Jong, Menno D T; Fennis, Bob M; Gosselt, Jordy F

    2009-06-01

    Two studies are reported addressing the media influences on adolescents' alcohol-related attitudes and behaviours. A content analysis was conducted to investigate the prevalence of alcohol portrayal in a Dutch soap series. The coding scheme covered the alcohol consumption per soap character, drinking situations and drinking times. Inter-coder reliability was satisfactory. The results showed that alcohol portrayal was prominent and that many instances of alcohol use reflected undesirable behaviours. To assess the influence of such alcohol cues on adolescents, a 2x2 experiment was conducted focusing on the separate and combined effects of alcohol portrayal in the soap series and surrounding alcohol commercials. Whereas the alcohol commercials had the expected effects on adolescents' attitudes, the alcohol-related soap content only appeared to have unexpected effects. Adolescents who were exposed to the alcohol portrayal in the soap series had a less positive attitude towards alcohol and lower drinking intentions. Implications of these findings for health policy and future research are discussed.

  3. Positive Portrayals of Feminist Men Increase Men's Solidarity with Feminists and Collective Action Intentions

    Science.gov (United States)

    Wiley, Shaun; Srinivasan, Ruhi; Finke, Elizabeth; Firnhaber, Joseph; Shilinsky, Alyssa

    2013-01-01

    The present study examined whether positive portrayals of feminist men could increase men's sense of solidarity with feminists and, through it, their intentions to engage in collective action in support of women. A sample of 102 mostly White men between the ages of 18 and 63 was recruited from Mechanical Turk, an online crowdsourcing marketplace.…

  4. Drench effects of media portrayal of fatal virus disease on health locus of control beliefs.

    Science.gov (United States)

    Bahk, C M

    2001-01-01

    Drawing on the notion of the drench hypothesis proposed by Greenberg (1988), the author proposes a preliminary theoretical framework to explain "drenching" effects of dramatic media. Three drench variables-perceived realism, role identification, and media involvement-were identified and tested regarding their role in mediating the impact of virus disease portrayals on health locus-of-control belief orientations. Participants in the experimental condition watched the movie Outbreak (a portrayal of an outbreak of a deadly virus disease). Perceived realism, role identification, and media involvement were measured concerning the movie depiction of the virus disease. The findings indicate that the dramatized portrayal significantly weakened the viewers' beliefs in self-controllability over health and strengthened their beliefs in chance outcomes of health. Beliefs in provider control over health were affected by the viewers' perception of realism regarding the movie portrayals. Effects of role identification were different between male and female viewers. The results are discussed in relation to drench analysis as a theoretical approach to media effects.

  5. The Aggressive Male versus the Passive Female: An Analysis of Differentials in Role Portrayal.

    Science.gov (United States)

    Higgs, Catriona T.; Weiller, Karen H.

    This study examined the role of women as portrayed in the mass media, focusing on the depiction of women in the traditionally passive role as compared with men in the traditionally aggressive role. The analysis of media coverage was highlighted by the examination of women and men in magazines, billboards, television, and newspapers between January…

  6. Portrayals of Bullying: A Content Analysis of Picture Books for Preschoolers

    Science.gov (United States)

    Oppliger, Patrice A.; Davis, Ashley

    2016-01-01

    Bullying affects a significant number of school children in the United States. Great concern for teaching children about bullying is apparent in the number of picture books published with bullying themes. The following study is a content analysis of how bullies and victims are portrayed in picture books suitable for preschoolers. Many of the…

  7. Portrayals of schizophrenia by entertainment media: a content analysis of contemporary movies.

    Science.gov (United States)

    Owen, Patricia R

    2012-07-01

    Critics of entertainment media have indicated that cinematic depictions of schizophrenia are stereotypic and characterized by misinformation about symptoms, causes, and treatment. The pervasiveness and nature of misinformation are difficult to ascertain because of the lack of empirically based studies of movies portraying schizophrenia. This study analyzed portrayals of schizophrenia in contemporary movies to ascertain prevalence of stereotypes and misinformation about schizophrenia. English-language movies featuring at least one main character with schizophrenia that were released for showing in theaters between 1990 and 2010 were analyzed for depictions of schizophrenia. Two researchers independently rated each character with a checklist that assessed demographic characteristics, symptoms and stereotypes, causation, and treatment. Forty-two characters from 41 movies were identified, a majority of whom were male and Caucasian. Most characters displayed positive symptoms of schizophrenia. Delusions were featured most frequently, followed by auditory and visual hallucinations. A majority of characters displayed violent behavior toward themselves or others, and nearly one-third of violent characters engaged in homicidal behavior. About one-fourth of characters committed suicide. Causation of schizophrenia was infrequently noted, although about one-fourth of movies implied that a traumatic life event was significant in causation. Of movies alluding to or showing treatment, psychotropic medications were most commonly portrayed. The finding that misinformation and negative portrayals of schizophrenia in contemporary movies are common underscores the importance of determining how viewers interpret media messages and how these interpretations inform attitudes and beliefs both of the general public and of people with schizophrenia.

  8. Developing Media Literacy Skills To Challenge Television's Portrayal of Older Women.

    Science.gov (United States)

    Cohen, Harriet L.

    2002-01-01

    Focus groups of 19 graduate social work students viewed two episodes of "The Golden Girls." Students had differing opinions on whether the program reinforced or challenged social stereotypes about older women. The need for positive media portrayals and for media literacy was identified. (Contains 46 references.) (SK)

  9. Effects of alcohol portrayals in movies on actual alcohol consumption: an observational experimental study

    NARCIS (Netherlands)

    Koordeman, R.; Anschutz, D.J.; van Baaren, R.B.; Engels, R.C.M.E.

    2011-01-01

    Aims This study uses an experimental design to assess the effects of movie alcohol portrayal on alcohol consumption of young adults while watching a movie. Gender, weekly alcohol use and identification with the movie actor/character were assessed as moderators. Design A two (sex) × two (movie:

  10. Effects of alcohol portrayals in movies on actual alcohol consumption: an observational experimental study

    NARCIS (Netherlands)

    Koordeman, R.; Anschutz, D.J.; Baaren, R.B. van; Engels, R.C.M.E.

    2011-01-01

    Aims - This study uses an experimental design to assess the effects of movie alcohol portrayal on alcohol consumption of young adults while watching a movie. Gender, weekly alcohol use and identification with the movie actor/character were assessed as moderators. Design - A two (sex) x two (movie:

  11. A 40-Year Portrait of the Portrayal of Industry on Prime-Time Television.

    Science.gov (United States)

    Trujillo, Nick; Ekdom, Leah R.

    1987-01-01

    Examines prime-time television's portrayals of industries from the 1950s through the 1980s. Claims that television overrepresents service and public administration industries and underrepresents manufacturing. Asserts that these representations reflect widely shared cultural values and the commercial constraints of network television. (MM)

  12. "The University for the Poor": Portrayals of Class in Translated Children's Literature

    Science.gov (United States)

    Forest, Danielle E.; Garrison, Kasey L.; Kimmel, Sue C.

    2015-01-01

    Background: Scholars of children's literature have been investigating portrayals of females and racial groups for several decades, yet few have examined depictions of social class. Research on social class depictions in children's literature is needed in order to identify books that affirm children's class identities and offer…

  13. Occupational Portrayal of Men and Women on the Most Frequently Mentioned Television Shows of Preschool Children.

    Science.gov (United States)

    Shechtman, Stephen A.

    The purpose of this study was to assess the distribution of male vs. female occupational portrayals in terms of occupational prestige on the six television shows most frequently mentioned by preschool children. The following shows were viewed and analyzed six times: Sesame Street, Bugs Bunny, Roadrunner, Batman, Flintstones, and Happy Days.…

  14. Effects of historically portrayed modeling and group treatment on self-observation: A comparison with agoraphobics

    NARCIS (Netherlands)

    Emmelkamp, Paul M.G.; Emmelkamp-Benner, Ank

    The effects of historically portrayed modeling and group treatment on self-observation were determined in a factorial design with agoraphobic patients. Group 1 saw a videofilm and was treated individually; group 2 saw the film and received group treatment; group 3 did not see the film and received

  15. Depictions and Gaps: Portrayal of U.S. Poverty in Realistic Fiction Children's Picture Books

    Science.gov (United States)

    Kelley, Jane E.; Darragh, Janine J.

    2011-01-01

    Researchers conducted a critical multicultural analysis of 58 realistic fiction children's picture books that portray people living in poverty and compared these depictions to recent statistics from the United States Census Bureau. The picture books were examined for the following qualities: main character, geographic locale and time era, focal…

  16. Developing Children and Multicultural Attitudes: The Systemic Psychosocial Influences of Television Portrayals in a Multimedia Society.

    Science.gov (United States)

    Berry, Gordon L.

    2003-01-01

    Television can be an important medium for the teaching and learning of the developing child. This article explores how social learning theory and the cross-cultural images and portrayals on television might influence the multicultural attitudes, values, and beliefs of children. (Contains 22 references.) (Author)

  17. Portrayal of Muslims in the media: “24” and the ‘Othering’ process

    OpenAIRE

    Abu Sadat Nurullah

    2010-01-01

    The Hollywood media in general depict the image of Muslims and Arabs in a negative way. Drawing from Edward Said's understanding of Orientalism, the current study critically analyzes the television serial "24", which portrays stereotypical images of Arabs and Muslims, and aggravates the 'othering process'.

  18. Effects of alcohol portrayals in movies on actual alcohol consumption: An observational experimental study

    NARCIS (Netherlands)

    Koordeman, R.; Anschutz, D.J.; Baaren, R.B. van; Engels, R.C.M.E.

    2011-01-01

    Aims - This study uses an experimental design to assess the effects of movie alcohol portrayal on alcohol consumption of young adults while watching a movie. Gender, weekly alcohol use and identification with the movie actor/character were assessed as moderators. Design - A two (sex) x two (movie:

  19. Occupational Portrayals on Television: Children's Role Schemata, Career Aspirations, and Perceptions of Reality.

    Science.gov (United States)

    Wright, John C.; And Others

    1995-01-01

    Examined whether children's schemata for occupations they observe in real life differ from schemata for occupations portrayed on television. Found that children do form separate schemata for television and real life. Compared to subjects' schemata of real life occupations, those for television versions entailed more glamour, higher income, more…

  20. Analysis of the Image of Scientists Portrayed in the Lebanese National Science Textbooks

    Science.gov (United States)

    Yacoubian, Hagop A.; Al-Khatib, Layan; Mardirossian, Taline

    2017-07-01

    This article presents an analysis of how scientists are portrayed in the Lebanese national science textbooks. The purpose of this study was twofold. First, to develop a comprehensive analytical framework that can serve as a tool to analyze the image of scientists portrayed in educational resources. Second, to analyze the image of scientists portrayed in the Lebanese national science textbooks that are used in Basic Education. An analytical framework, based on an extensive review of the relevant literature, was constructed that served as a tool for analyzing the textbooks. Based on evidence-based stereotypes, the framework focused on the individual and work-related characteristics of scientists. Fifteen science textbooks were analyzed using both quantitative and qualitative measures. Our analysis of the textbooks showed the presence of a number of stereotypical images. The scientists are predominantly white males of European descent. Non-Western scientists, including Lebanese and/or Arab scientists are mostly absent in the textbooks. In addition, the scientists are portrayed as rational individuals who work alone, who conduct experiments in their labs by following the scientific method, and by operating within Eurocentric paradigms. External factors do not influence their work. They are engaged in an enterprise which is objective, which aims for discovering the truth out there, and which involves dealing with direct evidence. Implications for science education are discussed.

  1. There's alcohol in my soap: Portrayal and effects of alcohol use in a popular television series.

    NARCIS (Netherlands)

    van Hoof, Joris Jasper; de Jong, Menno D.T.; Fennis, B.M.; Gosselt, Jordi Franciscus

    2009-01-01

    Two studies are reported addressing the media influences on adolescents’ alcohol-related attitudes and behaviours. A content analysis was conducted to investigate the prevalence of alcohol portrayal in a Dutch soap series. The coding scheme covered the alcohol consumption per soap character,

  2. Prime-Time Television's Portrayal of Women and the World of Work: A Demographic Profile.

    Science.gov (United States)

    Vande Berg, Leah R.; Streckfuss, Diane

    1992-01-01

    Reviews research on the portrayal of gender and occupation on prime-time television and describes a study that focused on representation and patterns of activity of male and female characters across the contextual variables of industry, occupational role, hierarchical position, plot function, genre, and dramatic tone. (36 references) (LRW)

  3. Korean Culture as Portrayed in Young Children's Picture Books: The Pursuit of Cultural Authenticity

    Science.gov (United States)

    Wee, Su-Jeong; Park, Soyeon; Choi, Joung Sun

    2015-01-01

    The purpose of the present study is to investigate how contemporary Korea and its culture are portrayed in children's picture books published in the United States. Our analysis of the representation of Korean culture in text and illustrations was based on a sample of 33 picture books written in English and published in the US between 1990 and…

  4. The Myth of My Widow: A Dramatistic Analysis of News Portrayals of a Terrorist Victim.

    Science.gov (United States)

    Lule, Jack

    Dramatistic analysis suggests that the "New York Times" portrayals of the 1985 terrorist killing of Leon Klinghoffer, the 69 year old American tourist on the Achille Laurs, may contain a mythic dimension. Through the myth of the hero, the news stories invoked the symbol of the self, inviting intense identification of the individual…

  5. The Portrayal of Women in Israeli Arabic Textbooks on Druse Heritage

    Science.gov (United States)

    Falah, Janan Faraj

    2013-01-01

    Teaching heritage to a nation is important. The Israeli Arab curriculum on Druse heritage helps students connect religion with modern society. The textbooks that are still used were written in the end of the 1970s and do not reflect the changing reality of Druse women. This research shows that women must be portrayed in more diverse roles in…

  6. Killing Us Softly? Investigating Portrayals of Women and Men in Contemporary Magazine Advertisements

    Science.gov (United States)

    Conley, Terri D.; Ramsey, Laura R.

    2011-01-01

    Our research aimed to systematically investigate how women and men are portrayed in magazine advertisements, deriving hypotheses from Jean Kilbourne's observed media analysis presented in her "Killing Us Softly" film series. A total of 790 advertisements in 19 magazines were coded. Results revealed support for many of Kilbourne's hypotheses. For…

  7. Magazine Portrayal of Women, 1911-1930. Journalism Monographs Number 72.

    Science.gov (United States)

    Hynes, Terry

    A total of 486 nonfiction reports and a proportional, stratified random sample of 300 short stories appearing in selected magazines from 1911 to 1930 were examined for the extent to which they portrayed or encouraged the emancipated woman. The study tested two assumptions: one, frequently made by writers commenting on the 1920s, that magazines of…

  8. How Real Is the Portrayal of Aggression in Television Entertainment Programming?

    Science.gov (United States)

    Potter, W. James; And Others

    1995-01-01

    Assesses aggressive behavior on television in terms of its realism. Replicated and contextualized reality were assessed for 100.5 hours of programming. Replicated reality compared television portrayals to real world characteristics, and was similar in seriousness to aggression and gender patterns of perpetrators and victims. Contextual reality…

  9. Correcting Media Mis-Education: The Portrayal of Smokers and Smoking in Top Grossing Films.

    Science.gov (United States)

    Bartlett, Alyssa; Brackin, Taryn; Chubb, Jamie; Covata, Sandy; Ferguson, Liz; Hinckley, Adele; Hodges, Jilda; Liberati, Cheryl; Tornetta, Jonette; Chambliss, Catherine

    Given that young people are extremely concerned with how they appear socially, beguiling and glamorous portrayals of smokers in recent films may be contributing to the continual rise in college student smoking. The pervasive positive depiction of smokers as attractive and appealing easily preys on young people who lack confidence and self esteem.…

  10. Patients, doctors, and videotape: a prescription for creating optimal healing environments?

    Science.gov (United States)

    Frankel, Richard M; Sung, Sue Hee; Hsu, John T

    2005-01-01

    Despite repeated calls for greater patient autonomy, shared decision making, and exploration of patient preferences, relatively little is known about how patients actually experience care as a face-to-face interactional process. A selected review of the literature in this area suggests that important asymmetries exist. Key among them is the tendency to report experiences from the point of view of only one member of the doctor-patient dyad. Thus, patients might report on their experiences of the system gone awry or professionals might attempt to understand the root cause(s) of an error by describing the conditions under which it occurred. Either way, information about the experience of care tends to be reported as a "my side" telling. Optimal healing environments are defined as health care contexts that are based upon mutual respect and build positive, resilient relationships among participants, using the qualities and resources of those relationships to enhance health. Understanding optimal healing environments also requires a knowledge of how doctors and patients share time and space together in the consultation (an etic or outsider perspective) and also a knowledge of the participants' experience of their time together (an emic or insider perspective). After reviewing its methodological roots, the IMPACT approach (Interactive Methodology for Preserving and Analyzing Clinical Transactions) using videotaped encounters along with independent commentaries by participants is described and applied in two different types of analyses: one in which the doctor-patient dyad is the unit of measure; the second in which physicians are stratified by having historically high or low satisfaction scores. In the latter approach, doctors' and patients' comments are compared across strata. At the dyadic level, and despite large gaps in income and social status, doctors and patients exhibit a strong tendency to cluster in terms of where they comment, so much so that in a pilot study

  11. Desperate Housewives: An Analysis of the Characterisations of Female Gamblers Portrayed in Gambling Movies in Hong Kong

    Science.gov (United States)

    Ohtsuka, Keis; Chan, Chi Chuen

    2009-01-01

    This article examines portrayals of female gamblers in recent Hong Kong movies. The authors report that the depiction of female gamblers is very different from that of male gamblers in the movies made in the same period. Whereas the male gamblers are pitching a lonely and desperate battle against an evil opponent, the female gamblers portrayed in…

  12. Trading Arms for Hostages? How the Government and Print Media "Spin" Portrayals of the United States' Policy toward Iran.

    Science.gov (United States)

    Brown, William J.; Vincent, Richard C.

    1995-01-01

    Analyzes government and print media portrayals of the Reagan administration's policy during Irangate. Finds that the print media continued to fulfill its "watchdog" function by providing critical portrayals of United States policy despite the Reagan administration's attempt to "spin" its own version of Irangate. Examines the…

  13. A Look at How Books, Reading, or Writing Are Portrayed in Children's Literature Published from 1990 through 1995.

    Science.gov (United States)

    Bushner, Diane E.

    This study examined 122 juvenile books to determine how current authors and illustrators portray books, reading, and writing and how this portrayal reflects current literacy theories and recommended instructional practices. The theoretical framework of this study is grounded theory (GLaser and Strauss, 1967). Categories which emerged from the…

  14. A Review and Critique of the Portrayal of Older Adult Learners in Adult Education Journals, 1980-2006

    Science.gov (United States)

    Chen, Li-Kuang; Kim, Young Sek; Moon, Paul; Merriam, Sharan B.

    2008-01-01

    The aging population is a worldwide challenge. Understanding how older adults have been portrayed would provide a foundation on which future scholarship can build. This study assesses and critiques the assumptions underlying the portrayal of older adults and their learning in adult education journals from 1980 to 2006. On reviewing 93 articles in…

  15. An Examination of Violence and Gender Role Portrayals in Video Games: Implications for Gender Socialization and Aggressive Behavior.

    Science.gov (United States)

    Dietz, Tracy L.

    1998-01-01

    Examines the portrayal of women and the use of violent themes in 33 popular video games. The analysis reveals that traditional gender roles and violence are central to many games. There were no female characters in 41% of games with characters, and women were portrayed as sex objects in 28% of these games. (SLD)

  16. A Study of Behavioral Change in 50 Severely Multi-Sensorily Handicapped Children Through Application of the Video-Tape Recorded Behavioral Evaluation Protocol. Final Report.

    Science.gov (United States)

    Curtis, W. Scott

    Examined with 49 deaf-blind children (under 9 years old) was the use of the Telediagnostic Behavior Evaluation Protocol, a video-tape recorded evaluation protocol. To further develop the Telediagnostic Protocol and delineate the characteristics of the Ss observed during the process of test development, Ss were video-taped in eight 3-minute…

  17. Gathering the Dreamers: The Transformation Process to a Learner-Centered School. The Reinventing School Series. Part Two and Viewing Guide. Videotape.

    Science.gov (United States)

    Burrello, Leonard C.; DiLaura, Nancy

    This videotape and viewing guide present an emerging learner-centered paradigm of teaching and learning and answer questions of why and how a staff changes its practices. The viewing guide describes the elementary school in the videotape, noting the full inclusion of 50 students identified as disabled, the team approach in which teachers are…

  18. Portrayals of branded soft drinks in popular American movies: a content analysis

    Directory of Open Access Journals (Sweden)

    Bell Robert A

    2006-03-01

    Full Text Available Abstract Background This study examines the portrayals of soft drinks in popular American movies as a potential vehicle for global marketing and an indicator of covert product placement. Methods We conducted a content analysis of America's top-ten grossing films from 1991 through 2000 that included portrayals of beverages (95 movies total. Coding reliabilities were assessed with Cohen's kappa, and exceeded 0.80. If there was at least one instance of branding for a beverage, the film was considered having branded beverages. Fisher's exact test was used to determine if soft drink portrayals were related to audience rating or genre. Data on the amount of time soft drinks appeared onscreen was log transformed to satisfy the assumption of normality, and analyzed using a repeated measures ANOVA model. McNemar's test of agreement was used to test whether branded soft drinks are as likely to appear or to be actor-endorsed compared to other branded beverages. Results Rating was not associated with portrayals of branded soft drinks, but comedies were most likely to include a branded soft drink (p = 0.0136. Branded soft drinks appeared more commonly than other branded non-alcoholic beverages (p = 0.0001, branded beer (p = 0.0004, and other branded alcoholic beverages (p = 0.0006. Actors consumed branded soft drinks in five times the number of movies compared to their consumption of other branded non-alcoholic beverages (p = 0.0126. About half the revenue from the films with portrayals of branded soft drinks come from film sales outside the U.S. Conclusion The frequent appearance of branded soft drinks provides indirect evidence that product placement is a common practice for American-produced films shown in the U.S. and other countries.

  19. Portrayals of branded soft drinks in popular American movies: a content analysis.

    Science.gov (United States)

    Cassady, Diana; Townsend, Marilyn; Bell, Robert A; Watnik, Mitchell

    2006-03-09

    This study examines the portrayals of soft drinks in popular American movies as a potential vehicle for global marketing and an indicator of covert product placement. We conducted a content analysis of America's top-ten grossing films from 1991 through 2000 that included portrayals of beverages (95 movies total). Coding reliabilities were assessed with Cohen's kappa, and exceeded 0.80. If there was at least one instance of branding for a beverage, the film was considered having branded beverages. Fisher's exact test was used to determine if soft drink portrayals were related to audience rating or genre. Data on the amount of time soft drinks appeared onscreen was log transformed to satisfy the assumption of normality, and analyzed using a repeated measures ANOVA model. McNemar's test of agreement was used to test whether branded soft drinks are as likely to appear or to be actor-endorsed compared to other branded beverages. Rating was not associated with portrayals of branded soft drinks, but comedies were most likely to include a branded soft drink (p = 0.0136). Branded soft drinks appeared more commonly than other branded non-alcoholic beverages (p = 0.0001), branded beer (p = 0.0004), and other branded alcoholic beverages (p = 0.0006). Actors consumed branded soft drinks in five times the number of movies compared to their consumption of other branded non-alcoholic beverages (p = 0.0126). About half the revenue from the films with portrayals of branded soft drinks come from film sales outside the U.S. The frequent appearance of branded soft drinks provides indirect evidence that product placement is a common practice for American-produced films shown in the U.S. and other countries.

  20. Crosstown: A Fox Family Channel Afternoon Special for Cable in the Classroom. [Videotape with] Guide for Educators.

    Science.gov (United States)

    2000

    "Crosstown," the television program featured in this videotape and teaching guide, depicts a teenager who is forced, due to her father's lack of child support payments, to leave her comfortable home four years after her parents' divorce and to move across town to an apartment in an inner city neighborhood. The teenager decides not to be…

  1. An evaluation of the effectiveness of a videotape programme on interobserver reliability in outcome assessment for osteoarthritis.

    Science.gov (United States)

    Bellamy, N; Anjema, C; Alikhan, N; Chhina, T; Dhanoa, D; Edelist, D; Esufali, Z; Ismail, F; Hill, J; Campbell, J

    1999-01-01

    A study was designed to assess the effects of a standardized instructional videotape on training senior medical students to acceptable levels of reliability in performing several commonly used observer dependent outcome measures in patients with osteoarthritis (OA). During a single day, six third-year medical students independently examined six patients with OA in predetermined order using a Latin Square design, before and after viewing a standardized videotape demonstrating 13 examination techniques. Reliability coefficients were calculated based on variance components of the analysis of variance (ANOVA) table. Preslandardization reliability coefficients were goniometry were uniformly low. Following the intervention, all but four reliability coefficients were >/= 0.93. For many measures, high levels of interobserver agreement were noted prior to viewing the instructional videotape. This may represent the success of undergraduate clinical skills training programmes, or it may be the result of having reviewed an illustrated instructional text just prior to the initial patient examinations. The notable exception was knee goniometry. Despite apparent familiarity with the technique, prestandardization reliability coefficients were very low. However, following the intervention, all coefficients improved dramatically, two-thirds achieving very high levels. These data suggest that skills development in senior medical students is not uniform and that, while reliability is high for many, the assessment of knee range of movement can be improved by viewing an instructional videotape.

  2. Natural transformation facilitates transfer of transposons, integrons and gene cassettes between bacterial species.

    Science.gov (United States)

    Domingues, Sara; Harms, Klaus; Fricke, W Florian; Johnsen, Pål J; da Silva, Gabriela J; Nielsen, Kaare Magne

    2012-01-01

    We have investigated to what extent natural transformation acting on free DNA substrates can facilitate transfer of mobile elements including transposons, integrons and/or gene cassettes between bacterial species. Naturally transformable cells of Acinetobacter baylyi were exposed to DNA from integron-carrying strains of the genera Acinetobacter, Citrobacter, Enterobacter, Escherichia, Pseudomonas, and Salmonella to determine the nature and frequency of transfer. Exposure to the various DNA sources resulted in acquisition of antibiotic resistance traits as well as entire integrons and transposons, over a 24 h exposure period. DNA incorporation was not solely dependent on integrase functions or the genetic relatedness between species. DNA sequence analyses revealed that several mechanisms facilitated stable integration in the recipient genome depending on the nature of the donor DNA; homologous or heterologous recombination and various types of transposition (Tn21-like and IS26-like). Both donor strains and transformed isolates were extensively characterized by antimicrobial susceptibility testing, integron- and cassette-specific PCRs, DNA sequencing, pulsed field gel electrophoreses (PFGE), Southern blot hybridizations, and by re-transformation assays. Two transformant strains were also genome-sequenced. Our data demonstrate that natural transformation facilitates interspecies transfer of genetic elements, suggesting that the transient presence of DNA in the cytoplasm may be sufficient for genomic integration to occur. Our study provides a plausible explanation for why sequence-conserved transposons, IS elements and integrons can be found disseminated among bacterial species. Moreover, natural transformation of integron harboring populations of competent bacteria revealed that interspecies exchange of gene cassettes can be highly efficient, and independent on genetic relatedness between donor and recipient. In conclusion, natural transformation provides a much

  3. Natural transformation facilitates transfer of transposons, integrons and gene cassettes between bacterial species.

    Directory of Open Access Journals (Sweden)

    Sara Domingues

    Full Text Available We have investigated to what extent natural transformation acting on free DNA substrates can facilitate transfer of mobile elements including transposons, integrons and/or gene cassettes between bacterial species. Naturally transformable cells of Acinetobacter baylyi were exposed to DNA from integron-carrying strains of the genera Acinetobacter, Citrobacter, Enterobacter, Escherichia, Pseudomonas, and Salmonella to determine the nature and frequency of transfer. Exposure to the various DNA sources resulted in acquisition of antibiotic resistance traits as well as entire integrons and transposons, over a 24 h exposure period. DNA incorporation was not solely dependent on integrase functions or the genetic relatedness between species. DNA sequence analyses revealed that several mechanisms facilitated stable integration in the recipient genome depending on the nature of the donor DNA; homologous or heterologous recombination and various types of transposition (Tn21-like and IS26-like. Both donor strains and transformed isolates were extensively characterized by antimicrobial susceptibility testing, integron- and cassette-specific PCRs, DNA sequencing, pulsed field gel electrophoreses (PFGE, Southern blot hybridizations, and by re-transformation assays. Two transformant strains were also genome-sequenced. Our data demonstrate that natural transformation facilitates interspecies transfer of genetic elements, suggesting that the transient presence of DNA in the cytoplasm may be sufficient for genomic integration to occur. Our study provides a plausible explanation for why sequence-conserved transposons, IS elements and integrons can be found disseminated among bacterial species. Moreover, natural transformation of integron harboring populations of competent bacteria revealed that interspecies exchange of gene cassettes can be highly efficient, and independent on genetic relatedness between donor and recipient. In conclusion, natural transformation

  4. Hygromycin B and apramycin antibiotic resistance cassettes for use in Campylobacter jejuni.

    Directory of Open Access Journals (Sweden)

    Andrew Cameron

    Full Text Available Campylobacter jejuni genetic manipulation is restricted by the limited number of antibiotic resistance cassettes available for use in this diarrheal pathogen. In this study, two antibiotic resistance cassettes were developed, encoding for hygromycin B and apramycin resistance, for use in mutagenesis or for selection of gene expression and complementation constructs in C. jejuni. First, the marker genes were successfully modified to allow for insertional mutagenesis or deletion of a gene-of-interest, and were bracketed with restriction sites for the facilitation of site-specific cloning. These hygromycin B and apramycin markers are encoded by plasmids pAC1H and pAC1A, respectively. We also modified an insertional gene-delivery vector to create pRRH and pRRA, containing the hygromycin B and apramycin resistance genes, and 3 unique restriction sites for the directional introduction of genes into the conserved multi-copy rRNA gene clusters of the C. jejuni chromosome. We determined the effective antibiotic concentrations required for selection, and established that no harmful effects or fitness costs were associated with carrying hygromycin B or apramycin resistance under standard C. jejuni laboratory conditions. Using these markers, the arylsulfatase reporter gene astA was deleted, and the ability to genetically complement the astA deletion using pRRH and pRRA for astA gene insertion was demonstrated. Furthermore, the relative levels of expression from the endogenous astA promoter were compared to that of polycistronic mRNA expression from the constitutive promoter upstream of the resistance gene. The development of additional antibiotic resistance cassettes for use in Campylobacter will enable multiple gene deletion and expression combinations as well as more in-depth study of multi-gene systems important for the survival and pathogenesis of this important bacterium.

  5. Cassette dosing for pharmacokinetic screening in drug discovery: comparison of clearance, volume of distribution, half-life, mean residence time, and oral bioavailability obtained by cassette and discrete dosing in rats.

    Science.gov (United States)

    Nagilla, Rakesh; Nord, Melanie; McAtee, Jeff J; Jolivette, Larry J

    2011-09-01

    The purpose of this investigation was to compare selected pharmacokinetic (PK) parameters obtained by cassette and discrete dosing of compounds in rats. The concordance of PK properties obtained by the two dosing strategies was evaluated for 116 compounds representing various therapeutic programs and diverse chemical structures. The correspondence between cassette- and discrete-dosing-derived PK properties was examined semiquantitatively and qualitatively. For semiquantitative comparison, compounds with cassette-to-discrete PK parameter ratios between 0.5 and 2 (inclusive) were considered to be in agreement. For qualitative comparison, compounds were divided into three categories (low, moderate, and high) based on the value of the PK parameter; compounds that fell into the same category following cassette and discrete dosing were considered to be in agreement. Of the 116 compounds evaluated, 89%, 91%, 80%, and 91% of the compounds were semiquantitatively equivalent for the intravenous PK parameters of clearance (CL), volume of distribution (Vdss), terminal elimination plasma half-life (HL), and mean residence time (MRT), respectively, whereas 79%, 80%, 79%, and 72% were qualitatively similar for CL, Vdss, MRT, and terminal elimination plasma HL, respectively. Following oral administration, bioavailability concordance was 72% when assessed qualitatively and 78% when determined semiquantitatively. Results from these analyses indicate that a cassette dosing strategy is a viable approach to screen compounds for PK properties within a drug discovery setting. Copyright © 2011 Wiley-Liss, Inc.

  6. Expression of heterologous genes from an IRES translational cassette in replication competent murine leukemia virus vectors

    DEFF Research Database (Denmark)

    Jespersen, Thomas; Duch, Mogens R.; Carrasco, M L

    1999-01-01

    We describe replication competent retroviruses capable of expressing heterologous genes during multiple rounds of infection. An internal ribosome entry site (IRES) from encephalomyocarditis virus was inserted in the U3 region of Akv- and SL3-3-murine leukemia viruses (MLV) to direct translation...... of neo or the enhanced green fluorescence protein gene (EGFP). Akv-MLV's with IRES-neo and IRES-EGFP cassettes replicated with titers of about 10(6) infectious units/ml while SL3-3-MLV with IRES-neo gave about 10(3)-fold lower titers. Interestingly, RNA analysis showed a drastic reduction in the amount...

  7. Marine integrons containing novel integrase genes, attachment sites, attI, and associated gene cassettes in polluted sediments from Suez and Tokyo Bays.

    Science.gov (United States)

    Elsaied, Hosam; Stokes, Hatch W; Kitamura, Keiko; Kurusu, Yasurou; Kamagata, Yoichi; Maruyama, Akihiko

    2011-07-01

    In order to understand the structure and biological significance of integrons and associated gene cassettes in marine polluted sediments, metagenomic DNAs were extracted from sites at Suez and Tokyo Bays. PCR amplicons containing new integrase genes, intI, linked with novel gene cassettes, were recovered and had sizes from 1.8 to 2.5 kb. This approach uncovered, for the first time, the structure and diversity of both marine integron attachment site, attI, and the first gene cassette, the most efficiently expressed integron-associated gene cassette. The recovered 13 and 20 intI phylotypes, from Suez and Tokyo Bay samples, respectively, showed a highly divergence, suggesting a difference in integron composition between the sampling sites. Some intI phylotypes showed similarity with that from Geobacter metallireducens, belonging to Deltaproteobacteria, the dominant class in both sampling sites, as determined by 16S rRNA gene analysis. Thirty distinct families of putative attI site, as determined by the presence of an attI-like simple site, were recovered. A total of 146 and 68 gene cassettes represented Suez and Tokyo Bay unsaturated cassette pools, respectively. Gene cassettes, including a first cassette, from both sampling sites encoded two novel families of glyoxalase/bleomycin antibiotic-resistance protein. Gene cassettes from Suez Bay encoded proteins similar to haloacid dehalogenases, protein disulfide isomerases and death-on-curing and plasmid maintenance system killer proteins. First gene cassettes from Tokyo Bay encoded a xenobiotic-degrading protein, cardiolipin synthetase, esterase and WD40-like β propeller protein. Many of the first gene cassettes encoded proteins with no ascribable function but some of them were duplicated and possessed signal functional sites, suggesting efficient adaptive functions to their bacterial sources. Thus, each sampling site had a specific profile of integrons and cassette types consistent with the hypothesis that the

  8. Media portrayal of nurses' perspectives and concerns in the SARS crisis in Toronto.

    Science.gov (United States)

    Hall, Linda McGillis; Angus, Jan; Peter, Elizabeth; O'Brien-Pallas, Linda; Wynn, Francine; Donner, Gail

    2003-01-01

    To describe nursing work life issues as portrayed in the media during the SARS crisis in Toronto. Content analysis of local and national news media documents in Canada. Media articles were sorted and classified by topic, and themes were identified. Themes were: (a) changing schemas of nursing practice: the new normal; (b) barriers to relational nursing work; (c) work life concerns: retention and recruitment; (d) nursing virtue: nurses as heroes and professionals; (e) paradoxical responses to nurses from the community; and (f) leadership in nursing during the SARS crisis. This analysis enhanced understanding of how nurses are portrayed in the media, but it indicated the significance of quality of work life and issues about work-home life. Some descriptions of the care and caring of nurses have made nursing seem like an important and influential profession to potential applicants who might previously have dismissed nursing as a career.

  9. How do patients at risk portray candidates for coronary heart disease? A qualitative interview study

    DEFF Research Database (Denmark)

    Frich, J.C.; Malterud, K.; Fugelli, P.

    2007-01-01

    OBJECTIVE: To explore how patients at risk of coronary heart disease (CHD) portray candidates for CHD. DESIGN: Qualitative interview study. SETTING: Norway. SUBJECTS: A total of 20 men and 20 women diagnosed with heterozygous familial hypercholesterolemia (FH) recruited through a lipid clinic. MAIN...... OUTCOME MEASURES: Participants' beliefs concerning persons who are considered candidates for CHD. RESULTS: Some participants believed that CHD could happen to anyone, while the majority conveyed detailed notions of persons they considered to be likely victims of CHD. Participants often portrayed...... the coronary candidate as someone who was different from themselves. Among those who mentioned gender, all presented the candidate as a man. Some women said that they had to reconcile themselves to being at risk of CHD, since they at first had conceived CHD as a man's disease. While some participants...

  10. The portrayal of heart disease in mass print magazines, 1991-2001.

    Science.gov (United States)

    Clarke, Juanne Nancarrow; Binns, Jeannine

    2006-01-01

    In this article we discuss the portrayal of heart disease based on a content analysis of the highest circulating English-language magazines available in Canada and published in Canada or the United States in 1991, 1996, and 2001. It includes both manifest and latent content analysis. In terms of the manifest analysis, the findings indicate the dominance of the medical frame followed by lifestyle and social structural frames. The latent analysis reveals the following frames: (a) optimism about medicine; (b) medicine as "good" and, by contrast, the body as "bad"; (c) heart disease as an "attack"; (d) heart disease as an individual responsibility; (e) contradictory information; (f) male celebrity patients and doctors; and (g) prestigious medical doctors, journals, and institutions. The medicalized portrayal of heart disease as fear generating is considered. In addition, the lack of attention to social structural causation in contrast to current epidemiological findings is discussed.

  11. Spanish Darwinian iconography: Darwin and evolutionism portrayed in Spanish press cartoons.

    Science.gov (United States)

    Domínguez, Martí; Mateu, Anna

    2013-11-01

    The theory of evolution has played a major role in the press since it was put forward by Charles Darwin in 1859. Its key role in biology and human philosophy is reflected by its presence in press cartoons, sections where the image of social reality is depicted in a more direct and satirical light. Through cartoons, artists have used their ingenuity or wit to portray one of the most controversial scientific figures of the past two centuries. This study examines the views portrayed by Spanish cartoonists about Charles Darwin and evolutionary theory in 2009, the bicentenary of the naturalist's birth and the celebration of 150 years since the publication of On the Origin of Species. These cartoons show how the controversy between Darwinism and religion remain latent in the heart of Spanish society, and how the figure of Darwin has become one of the main icons of science.

  12. Downregulation of ATP-binding cassette subfamily C member 4 increases sensitivity to neoadjuvant radiotherapy for locally advanced rectal carcinoma.

    Science.gov (United States)

    Yu, Zhi-Qi; Zhang, Chang; Wang, Hao; Lao, Xin-Yuan; Chai, Rui; Gao, Xian-Hua; Cao, Guang-Wen; Fu, Chuan-Gang

    2013-05-01

    This study was designed to verify the effect of ATP-binding cassette subfamily C member 4 on radiosensitivity of locally advanced rectal carcinoma. The expression of ATP-binding cassette subfamily C member 4 protein in 121 pretreatment tissue samples from locally advanced rectal carcinoma patients was detected by immunohistochemistry. Pathological response to radiotherapy was evaluated according to tumor regression grading by postoperative histological examinations after they received long-course preoperative neoadjuvant radiotherapy, and the association between clinicopathological data and tumor regression grading was analyzed retrospectively. For further validation, short hairpin RNA was constructed and transfected into colorectal carcinoma cell line HT29. The knockdown efficiency was confirmed at both RNA and protein levels. The altered radiosensitivity was evaluated by methylthiazolyl tetrazolium assay, colony formation assay, flow cytometry, and Hoechst 33258 staining. Univariate analysis revealed that ATP-binding cassette subfamily C member 4 expression (p member 4 expression (p member 4 expression efficiently and persistently. Downregulation of ATP-binding cassette subfamily C member 4 expression significantly enhanced inhibition of cell proliferation, decreased colony formation capacity, and increased cell apoptosis induced by irradiation, as examined by a series of experiments in vitro. In addition, radiobiological parameters calculated according to the single-hit multitarget model were also decreased significantly. Our data indicate that ATP-binding cassette subfamily C member 4 may be a useful molecular marker in predicting radiosensitivity, and a potential target in improving the response to neoadjuvant radiotherapy in locally advanced rectal carcinoma patients.

  13. Melodies and maladies: reflections on Shakespeare and his portrayal of disease and death

    Directory of Open Access Journals (Sweden)

    Pranab Chatterjee

    2014-06-01

    Full Text Available Shakespeare has long been acknowledged to be a pioneer in understanding the functions and dysfunctions of the human mind. However, very little discussion has centered on his knowledge of the physical science of medicine. In this article the authors reflect on Shakespeare’s portrayal of diseases and death in his work and conclude that he had significant knowledge of the contemporary medical sciences.

  14. "Is it realistic?" the portrayal of pregnancy and childbirth in the media.

    OpenAIRE

    Luce, Ann; Cash, Marilyn; Hundley, Vanora; Cheyne, H.; van Teijlingen, Edwin; Angell, Catherine

    2016-01-01

    BACKGROUND: Considerable debate surrounds the influence media have on first-time pregnant women. Much of the academic literature discusses the influence of (reality) television, which often portrays birth as risky, dramatic and painful and there is evidence that this has a negative effect on childbirth in society, through the increasing anticipation of negative outcomes. It is suggested that women seek out such programmes to help understand what could happen during the birth because there is ...

  15. Portrayal of Women’s Images in Television Dramas: A Malaysian Case

    OpenAIRE

    Ibrahim Faridah; Yunus Norzita; Halem Shah Dil Froz Jan Sayed; Ilias Munirah; Ahmad Mokhtar Amli Hazlin

    2017-01-01

    Current situations brought about by globalisation and sophisticated innovations in new technology not only have created a new understanding of the world around us but also conscientiously sensitised society, particularly the women towards a more active role in shaping their world. An important contributory factor that can determine the success of women is their centrality in the media, through media positions held or through media images portrayed. What this paper has endeavoured to show is t...

  16. A Critical Discourse Analysis of the media portrayal of Melania Trump as First Lady

    OpenAIRE

    Wallström, Sven

    2017-01-01

    Aim: To examine how the New York Times portrays Melania Trump in her role as First Lady. Methodology: A qualitative discourse analysis of newspaper articles from the New York Time’s online publication. The main theoretical and methodological foundation is Fairclough’s concept of critical discourse analysis (CDA) and Yvonne Hirdman’s theories about gender system and gender contract. Main results: The main results of the analysis is that Melania Trump is depicted as absent, non-traditional, irr...

  17. Portrayals of professionalism by the media: trends in etiquette and bedside manners as seen on television.

    Science.gov (United States)

    Gross, Anne F; Stern, Thomas W; Silverman, Benjamin C; Stern, Theodore A

    2012-01-01

    Critics of current healthcare argue that professionalism, as manifest by etiquette and bedside manners, has been eroding, in part as a consequence of portrayals on television (TV) and in the media. We sought to identify changing patterns of physicians' behaviors as shown on TV (as these interactions have often served as models for physicians-in-training) over the last 30 years. We selected popular TV shows that portrayed practicing physicians and analyzed doctor-family, doctor-doctor, and doctor-nurse interactions as well as methods of disclosing errors to identify changing behavioral trends. We found that difficult news was more commonly delivered while standing, and that handshakes were rarely offered to patients. Male physicians were seen raising their voices toward, disclosing errors to, as well as inappropriately touching, peers or subordinates. In comparison, female physicians were identified as raising their voices toward, disclosing errors to, as well as inappropriately touching, their supervisors. Over the past several decades, official salutations between physicians and nurses have become less common; physicians have started to address nurses solely by their first names. More recently, sexual banter and sexual activity have been portrayed as occurring predominantly between male physicians and female nurses. While shifts in behavioral patterns (in etiquette, bedside manners, and professionalism) of physicians as seen on television have not been radical, potentially concerning trends were identified. Media portrayals may change patients' perceptions of physicians, hospitals, and the health care profession as well as influence behaviors of medical trainees. Moreover, TV and the media can be used as teaching tools about professionalism in healthcare providers. Published by Elsevier Inc.

  18. Television fiction series targeted at young audience: plots and conflicts portrayed in a teen serie

    OpenAIRE

    García-Muñoz, Núria; Fedele, Maddalena

    2011-01-01

    This paper presents the main findings of a research project on teen series, which are television fiction series featuring teenagers and specifically targeted at a young audience. The analysis of the portrayal of young people in television fictional series specifically targeted at a young audience has a meaningful value both for television production and for audience reception. In fact, the potential consumers of the teen series –the teenagers– find themselves at a key moment in the constructi...

  19. Portrayals of Eating and Drinking in Popular American TV Programs: A Comparison of Scripted and Unscripted Shows.

    Science.gov (United States)

    Lee, Moon J; Gispanski, Lauren

    2016-05-01

    This study investigated portrayals of eating and drinking behaviors in popular American TV programs and compared scripted and unscripted (i.e., reality) shows. Through a content analysis of 95 episodes, the prevalence and nature of food/alcohol consumption that accompanied depictions of eating and drinking behaviors in 461 scenes were measured. Various foods were portrayed, but only 9% of foods portrayed were healthy (e.g., low in calories/fat content, such as fruits, vegetables, etc.). Approximately half of eating or drinking scenes either were accompanied by alcohol or contained solely alcoholic beverages. Significant differences between the scripted and unscripted shows were also found.

  20. Constructions of sexuality in later life: analyses of Canadian magazine and newspaper portrayals of online dating.

    Science.gov (United States)

    Wada, Mineko; Hurd Clarke, Laura; Rozanova, Julia

    2015-01-01

    Advertisements as well as contemporary literature and films often depict older adults as sexually undesirable and unattractive, which reinforces the stereotype that they are nonsexual. However, the evolving discourses of successful aging emphasize that active engagement in life is a key element of healthy aging and as such, have been influencing the ways that older adults' sexuality is represented. This paper explores how popular newspapers and magazines in Canada construct and portray later life sexuality within the context of online dating. We retrieved 144 newspaper and magazine articles about later life online dating that were published between 2009 and 2011. Our thematic and discursive analyses of the articles generated six themes. Of 144 articles, 13% idealized sexuality (sexual attractiveness and optimal sexual engagement) for older adults. The articles portrayed sexual interests and functioning as declining in later life (19%) more often than sustaining (15%). Approximately 15% of the articles suggested that older adults should explore new techniques to boost sexual pleasure, thereby medicalizing and ameliorating sexual decline. In addition, the articles challenged the stereotype of older adults as non-sexual and claimed that sexual engagement in later life was valuable as it contributed to successful aging. We address the paradox in the articles' positive portrayals of older adults' sexuality and the tensions that arise between the two distinct ideals of sexuality that they advance. Copyright © 2015 Elsevier Inc. All rights reserved.

  1. Room for improvement: mixed portrayal of young people with mental illness on Australian television news.

    Science.gov (United States)

    Henson, Connie; Chapman, Simon; McLeod, Lachlan; Johnson, Natalie; Hickie, Ian

    2010-03-01

    The aim of the present study was to review television news depiction of mental illness in children and adolescents to test the hypothesis that positive portrayals of adults with mental illness also apply to young people. Content and frame analysis of first-person depictions and overall news angle was carried out to determine whether depictions were positive, neutral or negative. The media portrayal of young people with mental illnesses was mixed. Overall, 10 items (29%) of news angles were positive, 13 items (37%) neutral and nine items (26%) negative. Positive themes were significantly less frequent for young people compared to adults (10 items, 29% compared to 151 items, 66%), and negative themes were more frequent (nine items, 26% vs 21 items, 9%). First-person depictions, however, were similar for young people and adults (24 items, 69% vs 150 items, 66%). An anti-medication sub-theme was notable. Overall the portrayal of young people with mental illnesses on television news is not as positive as that for adults. Similar to adults, however, first-person depictions resulted in a more positive representation of young people. Fewer first-person depictions in combination with an anti-medication theme and historically less access to appropriate services for young people may have contributed to the less positive results for young people with mental illnesses.

  2. Religious Orientation, Endorser Credibility, and the Portrayal of Female Nurses by the Media.

    Science.gov (United States)

    Lin, Chyong-Ling; Yeh, Jin-Tsann; Wu, Mong-Chun; Lee, Wei-Chung

    2015-10-01

    Medical consumption and media culture in Taiwan contain clear religious elements. It is common for people to believe that medicine is a supernatural treatment and to rely on thoughts of unseen power instead of rational consciousness. Religious-influenced patriarchy, seen in cultural gender roles, significantly influences religious adherents and degrades women as being part of a secondary class in society. As a contradictory tradition, women, in comparison to men, are considered best at undertaking certain jobs that require careful, detailed thought (such as nurses). Nursing and other occupations requiring a high degree of professionalism by women contradict the past religious-based concept of "ignorance is a woman's virtue." This study aims to probe female imagery in eastern and western Taiwan and explores whether religious culture and practice influences people's cognition of female nurses in advertising. The constructs are analyzed through structural equation modeling. Results reveal that religious followers do not necessarily trust female nurses more just because they are portrayed as professional medical specialists. Most consumers reflect this negative cognition through purchase intentions of products. For example, in comparing portrayals of attractiveness with portrayals of professionalism, attractiveness results in a better advertising effect. People with intrinsic or extrinsic religious orientation have gradually lowered their negative impressions of women; however, religious followers still more strongly insist on women's secondary position. Attractive female nurses are more likely judged as reliable, and this may be transferred to trust in their professional medical skills.

  3. "Is it realistic?" the portrayal of pregnancy and childbirth in the media.

    Science.gov (United States)

    Luce, Ann; Cash, Marilyn; Hundley, Vanora; Cheyne, Helen; van Teijlingen, Edwin; Angell, Catherine

    2016-02-29

    Considerable debate surrounds the influence media have on first-time pregnant women. Much of the academic literature discusses the influence of (reality) television, which often portrays birth as risky, dramatic and painful and there is evidence that this has a negative effect on childbirth in society, through the increasing anticipation of negative outcomes. It is suggested that women seek out such programmes to help understand what could happen during the birth because there is a cultural void. However the impact that has on normal birth has not been explored. A scoping review relating to the representation of childbirth in the mass media, particularly on television. Three key themes emerged: (a) medicalisation of childbirth; (b) women using media to learn about childbirth; and (c) birth as a missing everyday life event. Media appear to influence how women engage with childbirth. The dramatic television portrayal of birth may perpetuate the medicalisation of childbirth, and last, but not least, portrayals of normal birth are often missing in the popular media. Hence midwives need to engage with television producers to improve the representation of midwifery and maternity in the media.

  4. Portrayal of psychiatrists in Hindi movies released in the first decade of the 21st century.

    Science.gov (United States)

    Banwari, Girish H

    2011-09-01

    Of late, there has been an increase in the number of psychiatrists shown in Hindi movies. There is dearth of systematic research that focuses specifically upon the depiction of psychiatrists in Hindi cinema. To explore the portrayal of psychiatrists in recent Hindi cinema. Twenty-six Hindi movies released between January 2001 and March 2010 portraying 33 'psychiatrist' characters in a main or a secondary (but significant) role, were reviewed and data related to the personal attributes and professional conduct of the movie psychiatrists was collected. The portrayal of psychiatrists in Hindi movies released in the first decade of the 21st century is unflattering. Hindi movies depicted psychiatrists as most likely to be male, middle-aged, and friendly in their attitude towards the patients. 42.4% of them were clinically incompetent, and only 30.3% could make an accurate diagnosis. 39.4% of them breached professional ethics. Eight (24. 2%) transgressed non-sexual boundaries, whereas five (15.2%) violated both sexual and non-sexual boundaries. The most common clinical/treatment setting was outpatient (53.8%), and pharmacotherapy was the most common treatment modality used. Treatment outcome was depicted positive in only 23.1% of the movies analyzed. Copyright © 2011 Elsevier B.V. All rights reserved.

  5. Fully Automated Data Collection Using PAM and the Development of PAM/SPACE Reversible Cassettes

    Science.gov (United States)

    Hiraki, Masahiko; Watanabe, Shokei; Chavas, Leonard M. G.; Yamada, Yusuke; Matsugaki, Naohiro; Igarashi, Noriyuki; Wakatsuki, Soichi; Fujihashi, Masahiro; Miki, Kunio; Baba, Seiki; Ueno, Go; Yamamoto, Masaki; Suzuki, Mamoru; Nakagawa, Atsushi; Watanabe, Nobuhisa; Tanaka, Isao

    2010-06-01

    To remotely control and automatically collect data in high-throughput X-ray data collection experiments, the Structural Biology Research Center at the Photon Factory (PF) developed and installed sample exchange robots PAM (PF Automated Mounting system) at PF macromolecular crystallography beamlines; BL-5A, BL-17A, AR-NW12A and AR-NE3A. We developed and installed software that manages the flow of the automated X-ray experiments; sample exchanges, loop-centering and X-ray diffraction data collection. The fully automated data collection function has been available since February 2009. To identify sample cassettes, PAM employs a two-dimensional bar code reader. New beamlines, BL-1A at the Photon Factory and BL32XU at SPring-8, are currently under construction as part of Targeted Proteins Research Program (TPRP) by the Ministry of Education, Culture, Sports, Science and Technology of Japan. However, different robots, PAM and SPACE (SPring-8 Precise Automatic Cryo-sample Exchanger), will be installed at BL-1A and BL32XU, respectively. For the convenience of the users of both facilities, pins and cassettes for PAM and SPACE are developed as part of the TPRP.

  6. Ratiometric Matryoshka biosensors from a nested cassette of green- and orange-emitting fluorescent proteins.

    Science.gov (United States)

    Ast, Cindy; Foret, Jessica; Oltrogge, Luke M; De Michele, Roberto; Kleist, Thomas J; Ho, Cheng-Hsun; Frommer, Wolf B

    2017-09-05

    Sensitivity, dynamic and detection range as well as exclusion of expression and instrumental artifacts are critical for the quantitation of data obtained with fluorescent protein (FP)-based biosensors in vivo. Current biosensors designs are, in general, unable to simultaneously meet all these criteria. Here, we describe a generalizable platform to create dual-FP biosensors with large dynamic ranges by employing a single FP-cassette, named GO-(Green-Orange) Matryoshka. The cassette nests a stable reference FP (large Stokes shift LSSmOrange) within a reporter FP (circularly permuted green FP). GO- Matryoshka yields green and orange fluorescence upon blue excitation. As proof of concept, we converted existing, single-emission biosensors into a series of ratiometric calcium sensors (MatryoshCaMP6s) and ammonium transport activity sensors (AmTryoshka1;3). We additionally identified the internal acid-base equilibrium as a key determinant of the GCaMP dynamic range. Matryoshka technology promises flexibility in the design of a wide spectrum of ratiometric biosensors and expanded in vivo applications.Single fluorescent protein biosensors are susceptible to expression and instrumental artifacts. Here Ast et al. describe a dual fluorescent protein design whereby a reference fluorescent protein is nested within a reporter fluorescent protein to control for such artifacts while preserving sensitivity and dynamic range.

  7. CRISPR-Mediated Integration of Large Gene Cassettes Using AAV Donor Vectors

    Directory of Open Access Journals (Sweden)

    Rasmus O. Bak

    2017-07-01

    Full Text Available The CRISPR/Cas9 system has recently been shown to facilitate high levels of precise genome editing using adeno-associated viral (AAV vectors to serve as donor template DNA during homologous recombination (HR. However, the maximum AAV packaging capacity of ∼4.5 kb limits the donor size. Here, we overcome this constraint by showing that two co-transduced AAV vectors can serve as donors during consecutive HR events for the integration of large transgenes. Importantly, the method involves a single-step procedure applicable to primary cells with relevance to therapeutic genome editing. We use the methodology in primary human T cells and CD34+ hematopoietic stem and progenitor cells to site-specifically integrate an expression cassette that, as a single donor vector, would otherwise amount to a total of 6.5 kb. This approach now provides an efficient way to integrate large transgene cassettes into the genomes of primary human cells using HR-mediated genome editing with AAV vectors.

  8. Use of cassette-electrode microbial fuel cell for wastewater treatment.

    Science.gov (United States)

    Miyahara, Morio; Hashimoto, Kazuhito; Watanabe, Kazuya

    2013-02-01

    Cassette-electrode microbial fuel cells (CE-MFCs) have been developed for the conversion of biomass wastes into electric energy. The present study modified CE-MFC for its application to wastewater treatment and examined its utility in a long-term (240 days) experiment to treat a synthetic wastewater, containing starch, yeast extract, peptone, plant oil, and a detergent (approximately 500 mg of total chemical oxygen demand [COD] per liter). A test MFC reactor (1 l in capacity) was equipped with 10 cassette electrodes with total anode and cathode projection areas of 1440 cm(2), and the operation was initiated by inoculating with rice paddy-field soil. It was demonstrated that CE-MFC achieved COD removal rates of 80% at hydraulic-retention times of 6 h or greater, and electricity was generated at a maximum power density of 150 mW m(-2) and Coulombic efficiency of 20%. Microbial communities established on anodes of CEs were analyzed by pyrosequencing of PCR-amplified 16S rRNA gene fragments, showing that Geobacter, Clostridium, and Geothrix were abundantly detected in anode biofilms. These results demonstrate the utility of CE-MFC for wastewater treatment, in which Geobacter and Geothrix would be involved in the electricity generation. Copyright © 2012 The Society for Biotechnology, Japan. All rights reserved.

  9. CRISPR-Mediated Integration of Large Gene Cassettes Using AAV Donor Vectors.

    Science.gov (United States)

    Bak, Rasmus O; Porteus, Matthew H

    2017-07-18

    The CRISPR/Cas9 system has recently been shown to facilitate high levels of precise genome editing using adeno-associated viral (AAV) vectors to serve as donor template DNA during homologous recombination (HR). However, the maximum AAV packaging capacity of ∼4.5 kb limits the donor size. Here, we overcome this constraint by showing that two co-transduced AAV vectors can serve as donors during consecutive HR events for the integration of large transgenes. Importantly, the method involves a single-step procedure applicable to primary cells with relevance to therapeutic genome editing. We use the methodology in primary human T cells and CD34+ hematopoietic stem and progenitor cells to site-specifically integrate an expression cassette that, as a single donor vector, would otherwise amount to a total of 6.5 kb. This approach now provides an efficient way to integrate large transgene cassettes into the genomes of primary human cells using HR-mediated genome editing with AAV vectors. Copyright © 2017 The Author(s). Published by Elsevier Inc. All rights reserved.

  10. Electricity generation from cattle manure slurry by cassette-electrode microbial fuel cells.

    Science.gov (United States)

    Inoue, Kengo; Ito, Toshihiro; Kawano, Yoshihiro; Iguchi, Atsushi; Miyahara, Morio; Suzuki, Yoshihiro; Watanabe, Kazuya

    2013-11-01

    Cassette-electrode microbial fuel cells (CE-MFCs) are efficient and scalable devices for electricity production from organic waste. Previous studies have demonstrated that CE-MFCs are capable of generating electricity from artificial wastewater at relatively high efficiencies. In this study, a single-cassette CE-MFC was constructed, and its capacity for electricity generation from cattle manure suspended in water (solid to water ratio of 1:50) was examined. The CE-MFC reactor was operated in batch mode for 49 days; electricity generation became stable 2 weeks after initiating the operation. The maximum power density was measured at 16.3 W m⁻³ on day 26. Sequencing analysis of PCR-amplified 16S rRNA gene fragments obtained from the original manure and from anode biofilms suggested that Chloroflexi and Geobacteraceae were abundant in the anode biofilm (29% and 18%, respectively), whereas no Geobacteraceae sequences were detected in the original manure sample. The results of this study suggest that CE-MFCs can be used to generate electricity from water-suspended cattle manure in a scalable MFC system. Copyright © 2013 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

  11. Plug-and-Play Genetic Access to Drosophila Cell Types using Exchangeable Exon Cassettes

    Directory of Open Access Journals (Sweden)

    Fengqiu Diao

    2015-03-01

    Full Text Available Genetically encoded effectors are important tools for probing cellular function in living animals, but improved methods for directing their expression to specific cell types are required. Here, we introduce a simple, versatile method for achieving cell-type-specific expression of transgenes that leverages the untapped potential of “coding introns” (i.e., introns between coding exons. Our method couples the expression of a transgene to that of a native gene expressed in the cells of interest using intronically inserted “plug-and-play” cassettes (called “Trojan exons” that carry a splice acceptor site followed by the coding sequences of T2A peptide and an effector transgene. We demonstrate the efficacy of this approach in Drosophila using lines containing suitable MiMIC (Minos-mediated integration cassette transposons and a palette of Trojan exons capable of expressing a range of commonly used transcription factors. We also introduce an exchangeable, MiMIC-like Trojan exon construct that can be targeted to coding introns using the Crispr/Cas system.

  12. Self-control and the effects of movie alcohol portrayals on immediate alcohol consumption in male college students

    National Research Council Canada - National Science Library

    Koordeman, R; Anschutz, D.J; Engels, R.C.M.E

    2015-01-01

    .... Aims: The aim of the present study was to assess the immediate effects of movie alcohol portrayals on drinking of male social drinkers and to assess the moderating role of self-control in this relation...

  13. Self-portrayal concerns mediate the relationship between recalled teasing and social anxiety symptoms in adults with anxiety disorders.

    Science.gov (United States)

    Merrifield, Colleen; Balk, Daniel; Moscovitch, David A

    2013-06-01

    Previous research on individuals with anxiety disorders has demonstrated that both childhood peer maltreatment and concerns about negative self-portrayal are related to elevated symptoms of social anxiety (SA). In the present study, we examined whether concerns about negative self-portrayal might either moderate or mediate the relation between recalled childhood teasing history and current symptoms of SA in a non-treatment-seeking clinical sample of 238 individuals with anxiety disorders. Participants completed the Teasing Questionnaire-Revised (TQ-R), the Negative Self-Portrayal Scale (NSPS), and the Social Phobia Inventory (SPIN). Analyses using structural equation modeling (SEM) indicated that self-portrayal concerns mediated, but did not moderate, the relationship between recalled teasing and current SA, accounting for 51% of the total effect. Clinical implications and directions for future research are discussed. Copyright © 2013 Elsevier Ltd. All rights reserved.

  14. A videotaped intervention to enhance child control and reduce anxiety of the pain of dental injections.

    Science.gov (United States)

    Weinstein, P; Raadal, M; Naidu, S; Yoshida, T; Kvale, G; Milgrom, P

    2003-12-01

    While the psychological literature shows that perceptions of uncontrollability contribute to anxiety and other pathologies, interventions that enhance perceived control have been shown to reduce anxiety. This study attempted to assess a brief videotape to enhance child perceived control in a dental setting. 101 children aged 7-9 years completed warm-up procedures and viewed either: a) the experimental intervention, a 2 minutes video of a dentist explaining what an injection will feel like and proposing hand raising as a signal mechanism; or b) the control condition, a 2 minutes video of Disneyland. Fear of dental injections was assessed on a 10 cm visual analogue scale before and after the intervention. In the experimental group there was a significant fear reduction from pre- to post-intervention, while this was not the case in the control group. Children with higher pre-existing levels of fear benefited more from the intervention than children with lower levels of fear. The results of this pilot study suggest that intervention packages that impact child control have promise in lowering anxiety.

  15. Provider Education about Glaucoma and Glaucoma Medications during Videotaped Medical Visits

    Directory of Open Access Journals (Sweden)

    Betsy Sleath

    2014-01-01

    Full Text Available Objective. The purpose of this study was to examine how patient, physician, and situational factors are associated with the extent to which providers educate patients about glaucoma and glaucoma medications, and which patient and provider characteristics are associated with whether providers educate patients about glaucoma and glaucoma medications. Methods. Patients with glaucoma who were newly prescribed or on glaucoma medications were recruited and a cross-sectional study was conducted at six ophthalmology clinics. Patients’ visits were videotape recorded and patients were interviewed after visits. Generalized estimating equations were used to analyze the data. Results. Two hundred and seventy-nine patients participated. Providers were significantly more likely to educate patients about glaucoma and glaucoma medications if they were newly prescribed glaucoma medications. Providers were significantly less likely to educate African American patients about glaucoma. Providers were significantly less likely to educate patients of lower health literacy about glaucoma medications. Conclusion. Eye care providers did not always educate patients about glaucoma or glaucoma medications. Practice Implications. Providers should consider educating more patients about what glaucoma is and how it is treated so that glaucoma patients can better understand their disease. Even if a patient has already been educated once, it is important to reinforce what has been taught before.

  16. A Cassette Containing Thiostrepton, Gentamicin Resistance Genes, and dif sequences Is Effective in Construction of Recombinant Mycobacteria.

    Science.gov (United States)

    Mugweru, Julius; Makafe, Gaelle; Cao, Yuanyuan; Zhang, Yang; Wang, Bangxing; Huang, Shaobo; Njire, Moses; Chhotaray, Chiranjibi; Tan, Yaoju; Li, Xinjie; Liu, Jianxiong; Tan, Shouyong; Deng, Jiaoyu; Zhang, Tianyu

    2017-01-01

    The genetic manipulation of Mycobacterium tuberculosis genome is limited by the availability of selection markers. Spontaneous resistance mutation rate of M. tuberculosis to the widely used kanamycin is relatively high which often leads to some false positive transformants. Due to the few available markers, we have created a cassette containing thiostrepton resistance gene (tsr) for selection in M. tuberculosis and M. bovis BCG, and gentamicin resistance gene (aacC1) for Escherichia coli and M. smegmatis mc(2)155, flanked with dif sequences recognized by the Xer system of mycobacteria. This cassette adds to the limited available selection markers for mycobacteria.

  17. Alcohol portrayals in movies, music videos and soap operas and alcohol use of young people: Current status and future challenges

    OpenAIRE

    Koordeman, R.; Anschutz, D.J.; Engels, R.C.M.E.

    2012-01-01

    Aims: To provide an overview of studies of the effects of alcohol portrayals in movies, music videos and soap operas on alcohol consumption among young people. Moreover, we highlight important issues that need to be addressed in future research. Methods: This paper reviews the current literature on alcohol portrayals on-screen and the associated gaps and challenges in alcohol media research. Results: Thirteen longitudinal studies, 8 cross-sectional studies and 6 experimental studies examined ...

  18. Using case studies and videotaped vignettes to facilitate the development of critical thinking skills in new graduate nurses.

    Science.gov (United States)

    Hooper, Barbara L

    2014-01-01

    Critical thinking skills are an essential component of nursing and crucial to nursing practice. Case studies with videotaped vignettes were used to help facilitate the development of critical thinking skills in new graduate nurses. Results revealed a statistically significant increase (p = .041) on the overall Health Sciences Reasoning Test score. It is essential for educators to be aware of educational strategies that can affect the development of critical thinking skills.

  19. Alcohol portrayals in movies, music videos and soap operas and alcohol use of young people: current status and future challenges.

    Science.gov (United States)

    Koordeman, Renske; Anschutz, Doeschka J; Engels, Rutger C M E

    2012-01-01

    To provide an overview of studies of the effects of alcohol portrayals in movies, music videos and soap operas on alcohol consumption among young people. Moreover, we highlight important issues that need to be addressed in future research. This paper reviews the current literature on alcohol portrayals on-screen and the associated gaps and challenges in alcohol media research. Thirteen longitudinal studies, 8 cross-sectional studies and 6 experimental studies examined the effects of alcohol portrayals on-screen on alcohol consumption among young people. They showed a relation between on-screen alcohol exposure and onset and progression of alcohol consumption. A distinction can be made between long-term effects and immediate effects on alcohol consumption. Only lately, more attention has been paid to processes underlying the effects of on-screen alcohol exposure. Replication of findings and development of new research designs is essential. On-screen alcohol exposure does not affect everyone. It is important to test individual differences in susceptibility to on-screen alcohol portrayals. Further, not all media alcohol portrayal might provoke similar effects. It is therefore essential to test the effect of different types of alcohol portrayals.

  20. Jeff's Reef Oculina Banks Clelia Dive 607 2001 Digital Imagery - Captured from Videotapes taken during Submersible Dives to the Oculina Banks Deep Sea Coral Reefs (NODC Accession 0047190)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Digitial imagery, mpegs and jpegs, captured from mini-DV magnetic videotapes collected with an underwater 3-chip CCD color video camera, deployed from the research...

  1. Eau Gallie, Oculina Banks Clelia Dive 608 2001 Digital Imagery - Captured from Videotapes taken during Submersible Dives to the Oculina Banks Deep Sea Coral Reefs (NODC Accession 0047190)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Digitial imagery, mpegs and jpegs, captured from mini-DV magnetic videotapes collected with an underwater 3-chip CCD color video camera, deployed from the research...

  2. Sebastian Pinnacles Oculina Banks Clelia Dive 618 2001 Digital Imagery - Captured from Videotapes taken during Submersible Dives to the Oculina Banks Deep Sea Coral Reefs (NODC Accession 0047190)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Digitial imagery, mpegs and jpegs, captured from mini-DV magnetic videotapes collected with an underwater 3-chip CCD color video camera, deployed from the research...

  3. Cape Canaveral Oculina Banks Clelia Dive 616 2001 Digital Imagery - Captured from Videotapes taken during Submersible Dives to the Oculina Banks Deep Sea Coral Reefs (NODC Accession 0047190)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Digitial imagery, mpegs and jpegs, captured from mini-DV magnetic videotapes collected with an underwater 3-chip CCD color video camera, deployed from the research...

  4. Chapman's Reef Oculina Banks Clelia Dive 620 2001 Digital Imagery - Captured from Videotapes taken during Submersible Dives to the Oculina Banks Deep Sea Coral Reefs (NODC Accession 0047190)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Digitial imagery, mpegs and jpegs, captured from mini-DV magnetic videotapes collected with an underwater 3-chip CCD color video camera, deployed from the research...

  5. Sebastian Pinnacles, Oculina Banks Clelia Dive 614 2001 Digital Imagery - Captured from Videotapes taken during Submersible Dives to the Oculina Banks Deep Sea Coral Reefs (NODC Accession 0047190)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Digitial imagery, mpegs and jpegs, captured from mini-DV magnetic videotapes collected with an underwater 3-chip CCD color video camera, deployed from the research...

  6. Sebastian Pinnacles, Oculina Banks Clelia Dive 615 2001 Digital Imagery - Captured from Videotapes taken during Submersible Dives to the Oculina Banks Deep Sea Coral Reefs (NODC Accession 0047190)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Digitial imagery, mpegs and jpegs, captured from mini-DV magnetic videotapes collected with an underwater 3-chip CCD color video camera, deployed from the research...

  7. Eau Galllie Oculina Banks Clelia Dive 609 2001 Digital Imagery - Captured from Videotapes tken during Submersible Dives to the Oculina Banks Deep Sea Coral Reefs (NODC Accession 0047190)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Digitial imagery, mpegs and jpegs, captured from mini-DV magnetic videotapes collected with an underwater 3-chip CCD color video camera, deployed from the research...

  8. Chapman's Reef Oculina Banks Clelia Dive 621 2001 Digital Imagery - Captured from Videotapes taken during Submersible Dives to the Oculina Banks Deep Sea Coral Reefs (NODC Accession 0047190)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Digitial imagery, mpegs and jpegs, captured from mini-DV magnetic videotapes collected with an underwater 3-chip CCD color video camera, deployed from the research...

  9. Jeff's Reef Oculina Banks Clelia Dive 606 2001 Digital Imagery - Captured from Videotapes taken during Submersible Dives to the Oculina Banks Deep Sea Coral Reefs (NODC Accession 0047190)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Digitial imagery, mpegs and jpegs, captured from mini-DV magnetic videotapes collected with an underwater 3-chip CCD color video camera, deployed from the research...

  10. Cocoa Beach Oculina Banks Clelia Dive 617 2001 Digital Imagery - Captured from Videotapes taken during Submersible Dives to the Oculina Banks Deep Sea Coral Reefs (NODC Accession 0047190)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Digitial imagery, mpegs and jpegs, captured from mini-DV magnetic videotapes collected with an underwater 3-chip CCD color video camera, deployed from the research...

  11. Sebastian Pinnacles Oculina Banks Clelia Dive 619 2001 Digital Imagery - Captured from Videotapes taken during Submersible Dives to the Oculina Banks Deep Sea Coral Reefs (NODC Accession 0047190)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Digitial imagery, mpegs and jpegs, captured from mini-DV magnetic videotapes collected with an underwater 3-chip CCD color video camera, deployed from the research...

  12. Evaluation of urine-circulating cathodic antigen (Urine-CCA) cassette test for the detection of Schistosoma mansoni infection in areas of moderate prevalence in Ethiopia.

    Science.gov (United States)

    Erko, Berhanu; Medhin, Girmay; Teklehaymanot, Tilahun; Degarege, Abraham; Legesse, Mengistu

    2013-08-01

    To evaluate the diagnostic performance of antigen detecting urine-CCA cassette test for the detection of Schistosoma mansoni infection in areas of moderate prevalence in Ethiopia. Stool specimens were collected from 620 schoolchildren on three consecutive days. The samples were microscopically examined using double Kato slides; midstream urine specimens were also collected for three consecutive days and tested for S. mansoni. The sensitivity of the urine-CCA cassette test was determined using combined results of six Kato-Katz thick smears and three urine-CCA cassette tests as gold standard. The specificity of the urine-CCA cassette test was evaluated in an area where schistosomiasis is not endemic. Prevalence of S. mansoni infection as determined by single urine-CCA cassette test was 65.9%, by single Kato-Katz smear 37.3% and by six Kato-Katz thick smears 53.1% (P CCA cassette test was significantly (P CCA cassette test showed 100% specificity in endemic settings. In moderate and high prevalence areas, urine-CCA cassette test is more sensitive than the Kato-Katz method and can be used for screening and mapping of S. mansoni infection. © 2013 Blackwell Publishing Ltd.

  13. Effect of ATP-binding cassette subfamily B member 1 on bovine blastocyst implantation.

    Science.gov (United States)

    Mori, M; Kuwano, T; Kamori, T; Isozaki, Y; Nishihara, T; Yamauchi, N; Hattori, M-A

    2014-03-15

    The ATP-binding cassette subfamily B member 1 (ABCB1) is an efflux transporter that excretes xenobiotics and waste matter. High expression of ABCB1 induced by forskolin (FSK) and rifampicin (RIF) in the bovine blastocysts reportedly improves the cellular quality. In the present study, interferon-α, similar to FSK and RIF, was highly potent in inducing the expression of ABCB1 in the bovine blastocysts but did not exhibit an additive effect with FSK and RIF. Bovine blastocysts stimulated by the combined treatment with FSK, RIF, and interferon-α to express high levels of ABCB1 displayed better freezing resistance as indicated by higher cell numbers in post thawing cultures. On transfer to recipients, such embryos established pregnancies with significantly higher frequencies in repeat breeder cows rather than normal ones. Copyright © 2014 Elsevier Inc. All rights reserved.

  14. Chimeric proteins tagged with specific 3xHA cassettes may present instability and functional problems.

    Science.gov (United States)

    Saiz-Baggetto, Sara; Méndez, Ester; Quilis, Inma; Igual, J Carlos; Bañó, M Carmen

    2017-01-01

    Epitope-tagging of proteins has become a widespread technique for the analysis of protein function, protein interactions and protein localization among others. Tagging of genes by chromosomal integration of PCR amplified cassettes is a widely used and fast method to label proteins in vivo. Different systems have been developed during years in the yeast Saccharomyces cerevisiae. In the present study, we analysed systematically a set of yeast proteins that were fused to different tags. Analysis of the tagged proteins revealed an unexpected general effect on protein level when some specific tagging module was used. This was due in all cases to a destabilization of the proteins and caused a reduced protein activity in the cell that was only apparent in particular conditions. Therefore, an extremely cautious approach is required when using this strategy.

  15. Chimeric proteins tagged with specific 3xHA cassettes may present instability and functional problems.

    Directory of Open Access Journals (Sweden)

    Sara Saiz-Baggetto

    Full Text Available Epitope-tagging of proteins has become a widespread technique for the analysis of protein function, protein interactions and protein localization among others. Tagging of genes by chromosomal integration of PCR amplified cassettes is a widely used and fast method to label proteins in vivo. Different systems have been developed during years in the yeast Saccharomyces cerevisiae. In the present study, we analysed systematically a set of yeast proteins that were fused to different tags. Analysis of the tagged proteins revealed an unexpected general effect on protein level when some specific tagging module was used. This was due in all cases to a destabilization of the proteins and caused a reduced protein activity in the cell that was only apparent in particular conditions. Therefore, an extremely cautious approach is required when using this strategy.

  16. Selective substrate uptake: The role of ATP-binding cassette (ABC) importers in pathogenesis.

    Science.gov (United States)

    Tanaka, Kari J; Song, Saemee; Mason, Kevin; Pinkett, Heather W

    2018-04-01

    The uptake of nutrients, including metals, amino acids and peptides are required for many biological processes. Pathogenic bacteria scavenge these essential nutrients from microenvironments to survive within the host. Pathogens must utilize a myriad of mechanisms to acquire these essential nutrients from the host while mediating the effects of toxicity. Bacteria utilize several transport proteins, including ATP-binding cassette (ABC) transporters to import and expel substrates. ABC transporters, conserved across all organisms, are powered by the energy from ATP to move substrates across cellular membranes. In this review, we will focus on nutrient uptake, the role of ABC importers at the host-pathogen interface, and explore emerging therapies to combat pathogenesis. This article is part of a Special Issue entitled: Beyond the Structure-Function Horizon of Membrane Proteins edited by Ute Hellmich, Rupak Doshi and Benjamin McIlwain. Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.

  17. Role of family D ATP-binding cassette transporters (ABCD) in cancer.

    Science.gov (United States)

    Hlaváč, Viktor; Souček, Pavel

    2015-10-01

    ATP-binding cassette (ABC) transporters, belonging to the family D, are expressed in peroxisomes, endoplasmic reticulum or lysosomes. ABCD transporters play a role in transport of lipids, bile acids and vitamin B12 and associate with peroxisomal disorders. ABCD1 performs transport of coenzyme A esters of very-long-chain fatty acids (VLCFA) in peroxisomes and a number of mutations in ABCD1 gene were linked to an X-linked adrenoleucodystrophy (X-ALD). The role of ABCD transporters in tumour growth has not been studied in detail, but there is some evidence that ABCDs levels differ between undifferentiated stem or tumour cells and differentiated cells suggesting a possible link to tumorigenesis. In this mini-review, we discuss the available information about the role of ABCD transporters in cancer. © 2015 Authors; published by Portland Press Limited.

  18. Mechanical properties research of the cassette balance for impulse wind tunnel based on inertia compensation

    Science.gov (United States)

    Zhang, X. Q.; Lv, J. Z.; Wu, Y. C.

    2017-09-01

    The aerodynamic load signals acquired though the cassette force balance is transient while the model was tested in an impulse combustion wind tunnel. Therefore, it is necessary to explore the dynamic properties of the force balance. Firstly, it is simplified as a springs-damping-beam system, and then, the dynamic equation is deduced. Secondly, the mechanical properties of the force balance are researched through the finite element method. The output histories of the balance under step and sine loading are acquired. Results show that the mean elastic output of the balance approximates to the load exerting on it. Furthermore, the inertia compensation could not only improve the accuracy of mean output significantly, but also could reduce the transient error between output and input sharply.

  19. Cell and molecular biology of ATP-binding cassette proteins in plants.

    Science.gov (United States)

    Yazaki, Kazufumi; Shitan, Nobukazu; Sugiyama, Akifumi; Takanashi, Kojiro

    2009-01-01

    ATP-binding cassette (ABC) proteins constitute a large and diverse superfamily of membrane-bound and soluble proteins, which are involved in a wide range of biological processes in all organisms from prokaryotes to eukaryotes. Genome analyses of model plants, for example, Arabidopsis and rice, have revealed that plants have more than double numbers of this family member in their genomes compared to animals and insects. In recent years, various biochemical and physiological functions of ABC proteins in plants have been reported. Some are relevant for the defense mechanisms to biotic and abiotic stresses, whereas others are involved in the basic functions necessary for maintaining the plant life. Here, we provide an updated inventory of plant ABC proteins and summarize their tissue specificities, membrane localizations, and physiological functions.

  20. A precisely regulated gene expression cassette potently modulates metastasis and survival in multiple solid cancers.

    Directory of Open Access Journals (Sweden)

    Kun Yu

    2008-07-01

    Full Text Available Successful tumor development and progression involves the complex interplay of both pro- and anti-oncogenic signaling pathways. Genetic components balancing these opposing activities are likely to require tight regulation, because even subtle alterations in their expression may disrupt this balance with major consequences for various cancer-associated phenotypes. Here, we describe a cassette of cancer-specific genes exhibiting precise transcriptional control in solid tumors. Mining a database of tumor gene expression profiles from six different tissues, we identified 48 genes exhibiting highly restricted levels of gene expression variation in tumors (n = 270 compared to nonmalignant tissues (n = 71. Comprising genes linked to multiple cancer-related pathways, the restricted expression of this "Poised Gene Cassette" (PGC was robustly validated across 11 independent cohorts of approximately 1,300 samples from multiple cancer types. In three separate experimental models, subtle alterations in PGC expression were consistently associated with significant differences in metastatic and invasive potential. We functionally confirmed this association in siRNA knockdown experiments of five PGC genes (p53CSV, MAP3K11, MTCH2, CPSF6, and SKIP, which either directly enhanced the invasive capacities or inhibited the proliferation of AGS cancer cells. In primary tumors, similar subtle alterations in PGC expression were also repeatedly associated with clinical outcome in multiple cohorts. Taken collectively, these findings support the existence of a common set of precisely controlled genes in solid tumors. Since inducing small activity changes in these genes may prove sufficient to potently influence various tumor phenotypes such as metastasis, targeting such precisely regulated genes may represent a promising avenue for novel anti-cancer therapies.

  1. A snapshot of the UK media’s portrayal of non-ethnic citizens

    OpenAIRE

    Fallesen, Frederik; Inayat, Yasmine; Hohaus, Johanna

    2014-01-01

    This project is an analysis of the media landscape in the United Kingdom, where we analyze how the UK media is portraying its non-ethnic population – during the time period of 01.11.2014 to 30.11.2014. In order to do the analyses we operationialzed – critical discourse analysis (CDA) and Semetko’s and Valkenburg’s five different news frames: human interest, morality, conflict and responsibility – to develop our own method. We then structured the analysis in two parts: a quantitative and a qua...

  2. A Study on Gendered Portrayals in Children's Picture Books with Mathematical Content

    Directory of Open Access Journals (Sweden)

    Patricia R. Ladd

    2011-12-01

    Full Text Available This study analyzes sexism in children's picture books that incorporate mathematical problems and problem-solving into the plot to determine if children's earliest reading material is affecting the achievement gap between males and females in this subject area. The study focused not just on overall totals of male and female characters, but also analyzed which genders most often portrayed gender stereotyped behaviors and personality traits and which characters were most often shown with mathematical skills. The findings of the study show that there were twice as many male as female characters, and the math problem-solving was generally done by males in the majority of titles.

  3. Portrayal of neurological illness and physicians in the works of shakespeare.

    Science.gov (United States)

    Matthews, Brandy R

    2010-01-01

    William Shakespeare was arguably one of the most prolific writers of all time. The topics explored in his works include both physicians and neurological illnesses. In addition to a review of the portrayal of neurological diseases such as dementia, epilepsy, parkinsonism, and parasomnias, this article describes the roles of physicians in Shakespeare's plays. Furthermore, a novel hypothesis that King Lear, one of Shakespeare's more tragic figures, suffered from dementia with Lewy bodies is explored based on evidence from the dialogue of the drama. Copyright (c) 2010 S. Karger AG, Basel.

  4. Using video-taped examples of standardized patient to teach medical students taking informed consent

    Directory of Open Access Journals (Sweden)

    SHIRIN HABIBI KHORASANI

    2015-04-01

    Full Text Available Introduction: Medical student should be trained in medical ethics and one of the most essential issues in this field is taking informed consents. In this research, we compared the effect of effectiveness of teaching methods on students’ ability in taking informed consent from patients. Methods: This semi-experimental study was carried out on fifty eight subjects from the 4th-year students of Shiraz University of Medical Sciences who attended in medical ethics course before their ‘clinical clerkship’training.Method of sampling was census and students were randomly allocated into two groups of control group (n=28 was trained in traditional lecture-based class and the case groupnamed as A1 (n=22 were taught by video-taped examples of standardized patient.Then A1 group attended in traditional lecture-based classes named as A2. The groups were evaluated in terms the ability of recognition of ethical issues through the scenario based ethical examination before and after each training. Scenarios were related to the topics of informed consent. Data were analyzed by SPSS 14 software using descriptive statistics and anova test. P-value less than 0.05 was considered as significant. Results: The mean scores results of A2, A1 and B group were found to be 7.21, 5.91 and 5.73 out of 8, respectively. Comparison between the groups demonstrated that the ability of taking informed consent was significantly higher in A2 group (p<0.001, followed by A1 group (p<0.05, while was the least in the B group (p=0.875. Conclusion: According to this research, lecture-based teaching is still of great value in teaching medical ethics, but when combined with standardized patient, the outcome will be much better. It should be considered that mixed methods of teaching should be used together for better result.

  5. Integron gene cassettes and degradation of compounds associated with industrial waste: the case of the Sydney tar ponds.

    Directory of Open Access Journals (Sweden)

    Jeremy E Koenig

    Full Text Available Integrons are genetic platforms that accelerate lateral gene transfer (LGT among bacteria. They were first detected on plasmids bearing single and multiple drug resistance determinants in human pathogens, and it is abundantly clear that integrons have played a major role in the evolution of this public health menace. Similar genetic elements can be found in nonpathogenic environmental bacteria and in metagenomic environmental DNA samples, and it is reasonable to suppose that integrons have facilitated microbial adaptation through LGT in niches outside infectious disease wards. Here we show that a heavily impacted estuary, exposed for almost a century to products of coal and steel industries, has developed a rich and unique cassette metagenome, containing genes likely to aid in the catabolism of compounds associated with industrial waste found there. In addition, we report that the most abundant cassette recovered in this study is one that encodes a putative LysR protein. This autoregulatory transcriptional regulator is known to activate transcription of linked target genes or unlinked regulons encoding diverse functions including chlorocatechol and dichlorophenol catabolism. Finally, only class 1 integrase genes were amplified in this study despite using different primer sets, and it may be that the cassettes present in the Tar Ponds will prove to be associated with class 1 integrase genes. Nevertheless, our cassette library provides a snapshot of a complex evolutionary process involving integron-meditated LGT likely to be important in natural bioremediation.

  6. Molecular basis of multidrug transport by ATP-binding cassette transporters : A proposed two-cylinder engine model

    NARCIS (Netherlands)

    van Veen, HW; Higgins, CF; Konings, WN

    ATP-binding cassette multidrug transporters are probably present in all living cells, and are able to export a variety of structurally unrelated compounds at the expense of ATP hydrolysis. The elevated expression of these proteins in multidrug resistant cells interferes with the drug-based control

  7. Evidence for two interacting ligand binding sites in human multidrug resistance protein 2 (ATP binding cassette C2)

    NARCIS (Netherlands)

    Zelcer, Noam; Huisman, Maarten T.; Reid, Glen; Wielinga, Peter; Breedveld, Pauline; Kuil, Annemieke; Knipscheer, Puck; Schellens, Jan H. M.; Schinkel, Alfred H.; Borst, Piet

    2003-01-01

    Multidrug resistance protein 2 (MRP2) belongs to the ATP binding cassette family of transporters. Its substrates include organic anions and anticancer drugs. We have used transport assays with vesicles derived from Sf9 insect cells overproducing MRP2 to study the interactions of drugs, organic

  8. ROLE OF ATP BINDING CASSETTE SUB-FAMILY MEMBER 2 (ABCG2) IN MOUSE EMBRYONIC STEM CELL DEVELOPMENT.

    Science.gov (United States)

    ATP binding cassette sub-family member 2 (ABCG2), is a member of the ABC transporter superfamily and a principal xenobiotic transporter. ABCG2 is also highly expressed in certain stem cell populations where it is thought to be related to stem cell plasticity, although the role o...

  9. Design and construction of an in-plant activation cassette for transgene expression and recombinant protein production in plants.

    Science.gov (United States)

    Dugdale, Benjamin; Mortimer, Cara L; Kato, Maiko; James, Tess A; Harding, Robert M; Dale, James L

    2014-05-01

    Virus-based transgene expression systems have become particularly valuable for recombinant protein production in plants. The dual-module in-plant activation (INPACT) expression platform consists of a uniquely designed split-gene cassette incorporating the cis replication elements of Tobacco yellow dwarf geminivirus (TYDV) and an ethanol-inducible activation cassette encoding the TYDV Rep and RepA replication-associated proteins. The INPACT system is essentially tailored for recombinant protein production in stably transformed plants and provides both inducible and high-level transient transgene expression with the potential to be adapted to diverse crop species. The construction of a novel split-gene cassette, the inducible nature of the system and the ability to amplify transgene expression via rolling-circle replication differentiates this system from other DNA- and RNA-based virus vector systems used for stable or transient recombinant protein production in plants. Here we provide a detailed protocol describing the design and construction of a split-gene INPACT cassette, and we highlight factors that may influence optimal activation and amplification of gene expression in transgenic plants. By using Nicotiana tabacum, the protocol takes 6-9 months to complete, and recombinant proteins expressed using INPACT can accumulate to up to 10% of the leaf total soluble protein.

  10. The role of ATP-binding cassette (ABC) transporters in pathogenesis and multidrug resistance of the wheat pathogen Mycosphaerella graminicola

    NARCIS (Netherlands)

    Stergiopoulos, I.

    2003-01-01

    ATP-binding cassette (ABC) transporters are membrane proteins that utilise the energy derived from the hydrolysis of ATP to drive the transport of compounds over biological membranes. They are members of one of the largest protein families to date, present in both pro- and eukaryotic

  11. Portraying mental illness and drug addiction as treatable health conditions: effects of a randomized experiment on stigma and discrimination.

    Science.gov (United States)

    McGinty, Emma E; Goldman, Howard H; Pescosolido, Bernice; Barry, Colleen L

    2015-02-01

    Despite significant advances in treatment, stigma and discrimination toward persons with mental illness and drug addiction have remained constant in past decades. Prior work suggests that portraying other stigmatized health conditions (i.e., HIV/AIDS) as treatable can improve public attitudes toward those affected. Our study compared the effects of vignettes portraying persons with untreated and symptomatic versus successfully treated and asymptomatic mental illness and drug addiction on several dimensions of public attitudes about these conditions. We conducted a survey-embedded randomized experiment using a national sample (N = 3940) from an online panel. Respondents were randomly assigned to read one of ten vignettes. Vignette one was a control vignette, vignettes 2-5 portrayed individuals with untreated schizophrenia, depression, prescription pain medication addiction and heroin addiction, and vignettes 6-10 portrayed successfully treated individuals with the same conditions. After reading the randomly assigned vignette, respondents answered questions about their attitudes related to mental illness or drug addiction. Portrayals of untreated and symptomatic schizophrenia, depression, and heroin addiction heightened negative public attitudes toward persons with mental illness and drug addiction. In contrast, portrayals of successfully treated schizophrenia, prescription painkiller addiction, and heroin addiction led to less desire for social distance, greater belief in the effectiveness of treatment, and less willingness to discriminate against persons with these conditions. Portrayal of persons with successfully treated mental illness and drug addiction is a promising strategy for reducing stigma and discrimination toward persons with these conditions and improving public perceptions of treatment effectiveness. Copyright © 2014 Elsevier Ltd. All rights reserved.

  12. Older adults in prime-time television dramas in Taiwan: prevalence, portrayal, and communication interaction.

    Science.gov (United States)

    Lien, Shu-Chin; Zhang, Yan Bing; Hummert, Mary Lee

    2009-12-01

    A content and thematic analysis of 109 episodes (94.9 h) of prime-time dramas examined the portrayals of aging and the nature of intergenerational interaction involving older adults on Taiwanese television. The content analysis revealed that older characters, regardless of sex, appeared less frequently and in less prominent roles than other adult characters, but not in comparison to adolescents and children. The older characters who did appear, however, were predominantly portrayed as cognitively sound and physically healthy. The thematic analysis provided a different picture, showing that older characters talked about age explicitly, strategically linking it to death and despondence, to influence younger characters. Communication behavior themes identified included supporting, superiority, and controlling for older characters, and reverence/respect for younger characters. Findings are compared to those from similar studies of U.S. media and discussed from a Cultivation Theory perspective in terms of their reinforcement of Chinese age stereotypes and the traditional values of filial piety and age hierarchy in the context of globalization and culture change.

  13. Judicial Perceptions of Media Portrayals of Offenders with High Functioning Autistic Spectrum Disorders.

    Science.gov (United States)

    Berryessa, Colleen M

    In recent years, sensational media reporting focusing on crimes committed by those diagnosed with or thought to have High Functioning Autistic Spectrum Disorders (hfASDs) has caused societal speculation that there is a link between the disorder and violent criminality. No research exists on how and if the judiciary understands and is affected by this coverage. Therefore this study aims to examine how judges perceive and are influenced by media attention surrounding hfASDs and criminality. Semi-structured interviews were conducted with 21 California Superior Court Judges, including questions on media portrayal. Judges perceived general media portrayals of hfASDs in both positive and negative ways. However, almost all judges who had experienced media coverage surrounding hfASDs and criminality identified it as misleading and harmful to public perceptions of the disorder. These findings suggest judges are not exempt from media attention surrounding violence and hfASDs, and they recognize the potential adverse effects of this negative coverage. Although judges' report their opinions are not affected, the results demonstrate that judges are worried that the public and potentially other criminal justice actors are adversely affected and will continue to be moving forward.

  14. Internet comments elicited by media portrayal of a familicide-suicide case.

    Science.gov (United States)

    Sisask, Merike; Mark, Lauraliisa; Värnik, Airi

    2012-01-01

    Events of extraordinary violence attract media attention. Recently, much media reporting has moved to the Internet, giving readers the possibility to comment online. To analyze the content of public cognitions and the emotional tonality of attitudes expressed in spontaneous Internet comments on a familicide-suicide case reported in the Estonian media. A content analysis is based on the comments of 6,333 readers following media coverage. Descriptive statistics were used for quantitative data analysis. Of all comments, 27.6% were without manifest content. Manifest content categories were "value judgments" (35.2%), "risk and protective factors" (27.2%), and "critics of media portrayal" (10.0%). As to emotional tonality, the comments were most often angry (34.5%), ironic (24.7%), or neutral (23.3%). "Critics of media portrayal" had the most clearly pronounced emotional tonality, the majority being angry or approving. Comments about "value judgments" were most frequently anguished. An analysis of Internet comments can provide valuable information about value judgments and attitudes in society. From an ethical and preventive perspective, similar homicide and suicide stories should not be open for unmoderated public discussion. Nevertheless, the study represents an original methodological approach for such research and also provides some guidance for public relations campaigns.

  15. Media Portrayal of the Nursing Homes Sector: A Longitudinal Analysis of 51 U.S. Newspapers.

    Science.gov (United States)

    Miller, Edward Alan; Livingstone, Ian; Ronneberg, Corina R

    2017-06-01

    Most Americans' low opinion of the nursing home (NH) sector could derive, in part, from the way in which it is portrayed in the media. This study furthers understanding of media portrayal of the NH sector by identifying how NHs were depicted in 51U.S. newspapers from 1999 to 2008. Keyword searches of the LexisNexis database were performed to identify 16,280 NH-related articles. Article content was analyzed, and tone, themes, prominence, and central actor were assessed. Basic frequencies and descriptive statistics were used to examine article content across regions, market type, and over time. Findings reveal considerably less NH coverage in the Western United States and a steady decline in NH coverage nationally over time. Most articles were news stories; more than one third were located on the front page of the newspaper or section. Most articles focused on NH industry and government interests, very few on residents/family and community concerns. Most articles were neutral or negative in tone; very few were positive or mixed. Common themes included quality, financing, and legal concerns. Tone, themes, and other article attributes varied across region, market type, and over time. Overall, findings reveal changes in how newspapers framed NH coverage, not only with respect to tone but also with respect to what dimensions of this complex issue have been emphasized during the time period analyzed. Variation in media coverage may contribute to differences in government and public views toward the NH sector across regions and over time.

  16. Priority setting in health care as portrayed in South Korean and Israeli newspapers.

    Science.gov (United States)

    Factor, Roni; Kang, Minah

    2014-02-01

    Studies have reported differences in the public's understanding of, trust in, and satisfaction with its priority-setting processes and outcomes across countries. How the media frames and reports decision making processes and outcomes may both reflect and affect the public's knowledge of and attitudes toward them. Nevertheless, no studies have analyzed how priority-setting decision making processes are portrayed in the media. We analyzed 202 newspaper articles published over a decade, from January 2000 through December 2009, in leading newspapers of Israel and South Korea. The findings reveal intriguing differences between the countries in both the number and content of the reports. The issue of priority setting is much less salient in Korean than in Israeli society. While the complexity of the task was the most prevalent theme in the Israeli reports sampled, benefits package expansion decisions were most common in the Korean reports. Similarly, the Israeli reports emphasized the qualifications and backgrounds of individual members of the decision making committee, but the equivalent Korean committee was not portrayed as a major actor, and so received less attention. The least reported theme in both countries was priority-setting procedures and principles. These findings, along with results from previous studies which indicate that public satisfaction with the two systems differs between the countries, provoke several interesting future research questions. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

  17. "Forty is the new twenty": An analysis of British media portrayals of older mothers.

    Science.gov (United States)

    Mills, Tracey A; Lavender, Rebecca; Lavender, Tina

    2015-06-01

    Despite increased risks of infertility and poor outcomes, women in high-income countries are increasingly deferring pregnancy beyond age 35.The underlying causes are incompletely understood. The mass media is recognised as a powerful influence on health-related behaviour; therefore media representations warrant critical examination. Qualitative thematic analysis of portrayals of pregnancy and birth in women over 35 in UK national newspapers, popular magazines and television programmes Extensive media interest in childbearing and a preponderance of coverage related to celebrities was confirmed. Three main themes were identified; delayed childbearing was portrayed positively, as part of a life plan allowing women have the "best of both worlds". The media did not acknowledge age per sé as an obstacle to pregnancy and endorsed reproductive technologies suggesting 'it's never too late'. Images and descriptions of ideal post-pregnancy bodies in women over 35 reinforced the message that "you can regain your beautiful body". Delayed childbearing was represented positively, as it facilitated conformity with dominant ideologies surrounding motherhood. Within these boundaries, the effects of age were disregarded. This study provides evidence of restrictive framing of the issues surrounding delayed childbearing in the UK media which militate against communication of important public health messages. Copyright © 2014 Elsevier B.V. All rights reserved.

  18. The portrayal of infant feeding in British women's magazines: a qualitative and quantitative content analysis.

    Science.gov (United States)

    O'Brien, E; Myles, P; Pritchard, C

    2017-06-01

    Exclusive breastfeeding is the best start an infant can receive. However, in many high-income countries breastfeeding rates are low and this may be a reflection of social norms which in turn may be influenced by the media. This study therefore explored the portrayal of infant feeding in women's general interest magazines. The five top selling women's weekly magazines in Britain and Ireland for 2013 over a 4-month period were included. A quantitative and qualitative content analysis was conducted for both written and visual content. In 58 magazines, there were 90 references to infant feeding with an average of 1.5 (range: 0-5) per magazine. Breastfeeding and formula feeding references were present in equal number and both were predominantly portrayed positively. There was only 1 visual representation of breast feeding compared with 11 of bottle feeding. Potential drivers for breastfeeding included its role in post-pregnancy weight loss and celebrity endorsement while family routine, the role of males in the house and concerns about adverse health effects were identified as barriers to breastfeeding. An improvement in visual representations of breast feeding and factual information in women's weekly magazines may be helpful in re-defining social norms regarding infant feeding. Keywords: food and nutrition, health promotion, public health.

  19. Palliative and terminal care at home as portrayed in Dutch newspapers in 2009 compared to 2000.

    Science.gov (United States)

    Van den Berg, Rianne; Eliel, Miriam R; Meijman, Frans J

    2011-03-01

    Terminal care at home is receiving increased attention from the Dutch media, which is relevant for primary care providers. To explore the portrayal of terminal care at home in newspapers in the Netherlands in 2000 and in 2009. We performed a systematic analysis of documents retrieved from the LexisNexis Academic NL database. We compared data regarding the 'point of reference' (or 'frame') and the 'moral judgments' in articles appearing in the first six months of 2009 to the same period in 2000. In the year 2000, the framing of articles in regional newspapers appeared to be predominantly consumer-oriented (66%) and in national newspapers predominantly contextual (63%). The moral judgment in the regional articles was predominantly positive (66%), in the national newspapers predominantly negative (58%). In 2009, articles in regional and national newspapers were categorized mainly as consumer-oriented (73% and 55%, respectively), and new themes appeared, i.e. self care and medical topics. For moral judgment, again regional articles were mainly positive (76%) and national articles were mainly negative (39%) and neutral (31%). Regarding the theme 'terminal care at home' as published in Dutch newspapers in 2009 as compared to 2000, the diversity of the framing was more prominent and over all moral judgments were more positive, particularly in regional newspapers. Health care providers should realize that the portrayal of palliative and terminal care at home may differ depending on the type of newspaper--regional or national.

  20. Development of remote pipe welding tool for divertor cassettes in JT-60SA

    Energy Technology Data Exchange (ETDEWEB)

    Hayashi, Takao, E-mail: hayashi.takao@jaea.go.jp [Fusion Research and Development Directorate, Japan Atomic Energy Agency, Naka (Japan); Sakurai, Shinji; Sakasai, Akira; Shibanuma, Kiyoshi [Fusion Research and Development Directorate, Japan Atomic Energy Agency, Naka (Japan); Kono, Wataru; Ohnawa, Toshio; Matsukage, Takeshi [Toshiba Corporation, Yokohama, Kanagawa (Japan)

    2015-12-15

    Highlights: • Remote pipe welding tool accessing from inside of the pipe has been newly developed. • Cooling pipe with a jut on the edge expands the acceptable welding gap up to 0.5 mm. • Positioning accuracy of the laser beam is realized to be less than ±0.1 mm. • We have achieved robust welding for an angular misalignment of 0.5°. - Abstract: Remote pipe welding tool accessing from inside of the pipe has been newly developed for JT-60SA. Remote handling (RH) system is necessary for the maintenance and repair of the divertor cassette in JT-60SA. Because the space around the cooling pipe connected with the divertor cassette is very limited, the cooling pipe is to be remotely cut and welded from inside for the maintenance. A laser welding method was employed to perform circumferential welding by rotating the focusing mirror inside the pipe. However, the grooves of connection pipes are not precisely aligned for welding. The most critical issue is therefore to develop a reliable welding tool for pipe connection without a defect such as undercut weld due to a gap caused by angular and axial misalignments of grooves. In addition, an angular misalignment between two pipes due to inclination of pipe has to be taken into account for the positioning of the laser beam during welding. In this paper, the followings are proposed to solve the above issues: (1) Cooling pipe connected with the divertor is machined to have a jut on the edge so as to expand the acceptable welding gap up to 0.5 mm by filling the gap with welded jut. (2) Positioning accuracy of the laser beam for reliable welding is realized to be less than ±0.1 mm along the circumferential target for welding by a position control mechanism provided in the tool even in the case of up to angular misalignment of 0.5° between connection pipes. Based on the above proposals, we have achieved robust welding for a large gap up to 0.5 mm as well as the maximum angular misalignment of 0.5° between connection pipes

  1. Gender Asymmetries Encountered in the Search and Exploration of Mining Engineering Program Web Sites: a Portrayal of Posture and Roles

    Science.gov (United States)

    Banning, James H.; Sexton, Julie; Most, David E.; Maier, Shelby

    Photographs found in the search for and exploration of 13 university mining engineering department Web sites were studied for their asymmetries of power by analyzing the role (student, instructor, secretarial staff, miner, and honoree) and posture (sitting, standing) of men and women in the photographs. The Web site photographs showed a higher rate of women occupying student roles than men did. Women had a lower rate of occupying instructor and miner roles. No women were portrayed as being honored. Men exhibited a higher rate of occupying the standing posture than did women. Women were more often shown sitting than men were. Implications of portraying a nonequitable power structure between men and women in the search for and exploration of mining engineering Web sites are discussed, including a recommendation that all academic departments should examine the portrayal of gender on their Web sites.

  2. Booze, drugs, and pop music: trends in substance portrayals in the billboard top 100-1968-2008.

    Science.gov (United States)

    Christenson, Peter; Roberts, Donald F; Bjork, Nicholas

    2012-01-01

    This paper presents the results of a content analysis of alcohol and drug portrayals in the top 100 Billboard songs from each of the years 1968, 1978, 1988, 1998, and 2008, thus allowing both a characterization of substance portrayals in music generally and an analysis of changes over time. Of the final sample of 496 songs, 10.3% contained a reference to alcohol and 5.7% contained a reference to drugs. A substantial increase was found over the decades, and in particular over the last two: in 1988, 12% of songs referred to either or both classes of substance, compared to 30% in 2008. Marijuana was by far the most frequently mentioned drug. Both alcohol and drugs were much more likely to be portrayed positively than negatively, especially in recent decades. The results are discussed in terms of relevant theories of media processing and impact.

  3. The Influence of Non-Misogynous and Mixed Portrayals of Intimate Partner Violence in Music on Beliefs About Intimate Partner Violence.

    Science.gov (United States)

    Franiuk, Renae; Coleman, Jill; Apa, Bethany

    2016-03-15

    In this study, we investigated the effect of songs that offer non-misogynous and ambivalent portrayals of intimate partner violence (IPV). Participants (N = 103) were exposed to a misogynous song about IPV, a song critical of IPV, and a song that offered an ambivalent portrayal of IPV. Our results showed positive effects of the anti-IPV song, and both positive and negative effects of the ambivalent portrayal on participants' beliefs about a violent relationship. These findings suggest that the context in which IPV is portrayed should be considered when evaluating the impact of media depicting IPV. © The Author(s) 2016.

  4. A Portrayal of Victorian Men and Women as Seen in O'neill's “the Grand Match”

    OpenAIRE

    Kurnia, Nandy Intan

    2011-01-01

    This paper analyzes the portrayal of Victorian women and men in the poem of Moira O'Neill, entitled “The Grand Match”. The poem gives the description of men and women relationship related to the discussion of love and marriage. As one of Victorian female poets, O'Neill tries to satirize the relationship of women and men by portraying the love story of a stereotypical male fortune seeker, who marries a rich woman and denies his own feeling toward a young-charming woman. This study is an attemp...

  5. The portrayal of complementary and alternative medicine in mass print magazines since 1980.

    Science.gov (United States)

    Clarke, Juanne; Romagnoli, Amy; Sargent, Cristal; van Amerom, Gudrun

    2010-01-01

    The objectives of this study were to examine and describe the portrayal of complementary and alternative medicine (CAM) in mass print media magazines. The sample included all 37 articles found in magazines with circulation rates of greater than 1 million published in the United States and Canada from 1980 to 2005. The analysis was quantitative and qualitative and included investigation of both manifest and latent magazine story messages. Manifest analysis noted that CAM was largely represented as a treatment for a patient with a medically diagnosed illness or specific symptoms. Discussions used biomedical terms such as patient rather than consumer and disease rather than wellness. LATENT analysis revealed three themes: (1) CAMs were described as good but not good enough; (2) individualism and consumerism were venerated; and (3) questions of costs were raised in the context of confusion and ambivalence.

  6. Portrayal of mental illness and special hospitals in the UK press.

    Science.gov (United States)

    Dickens, Geoff

    The portrayal of mental illness in the media can be negative and stigmatizing. Mental health nurses, therefore, need to be aware of the ways in which the media frame mental illness issues, and should be prepared to challenge inaccuracy. This article examines the changing nature of the UK press's reporting of mental health issues by focusing on two areas. First, the changing terminology the press uses in their attempt to appear more sensitive, exemplified by recent growth in use of the term 'suicide watch'. Secondly, the article examines the press' reporting of the three English high-security special hospitals as an example of how the framing and personalization of stories is used to set the public agenda on mentally disordered offenders.

  7. Newspaper portrayals of health and illness among Canadian seniors : Who ages healthily and at what cost?

    Directory of Open Access Journals (Sweden)

    Julia Rozanova

    2006-12-01

    Full Text Available While media representations of health and illness receive growing atten-tion from researchers, few studies have considered the newspaper por-trayals of health and illness among the elderly. Yet, print media are one vehicle through which governments, in a climate of concern about population aging and the sustainability of the social safety net, empha-size individual responsibility for health and well-being in later life. By praising healthy aging, the media may, perhaps inadvertently, perpetu-ate new ageist stereotypes that marginalize vulnerable adults who fail to age healthily, and downplay the role of social institutions and structural inequalities (particularly gender and socio-economic status in influenc-ing individuals’ personal resources and lifestyle choices. This paper explores whether, and if so, how the media represent interrelations between health and aging, through thematic analysis of a pool of articles about seniors published in The Globe and Mail in 2005.

  8. Bollywoodization of foreign policy: How film discourse portrays tension between states

    Directory of Open Access Journals (Sweden)

    Azmat Rasul

    2015-07-01

    Full Text Available As the nexus between film industry and state apparatus has grown critical and complex in the wake of war on terror, academic circle have paid attention to identify the patterns of relationship between entertainment industry of a country and its foreign policy. To understand the role of the soft power in assisting governments to secure their interests at international level, this article examines the relationship between Bollywood and the Indian foreign policy through the lens of critical political economy of communication approach. Popular films portraying tension and cooperation between the South Asian neighbors were critically analyzed, and the results indicated that Bollywood closely followed the foreign policy initiative of the Indian government.

  9. Rejected by Family for Being Gay or Lesbian: Portrayals, Perceptions, and Resilience.

    Science.gov (United States)

    Carastathis, Geoffrey S; Cohen, Lynne; Kaczmarek, Elizabeth; Chang, Paul

    2017-01-01

    A myriad of negative psychosocial outcomes face gay men and lesbians who experience rejection by their family for not being heterosexual. However, what constitutes rejection is not clear, and, more importantly, it is not known how resilience is fostered within such a context. This qualitative study sought to examine these issues by exploring the lived experiences of Australian gay men and lesbians who were previously or were currently experiencing family rejection because of their sexuality. A total of 21 in-depth, semistructured interviews were individually conducted and analyzed. Thematic analysis of the interviews revealed several themes that provided greater understanding of the portrayals and perceptions of family rejection; the impact of family rejection on mental health; and, importantly, how these individuals came to establish resilience in the face of experiencing family rejection. Limitations, directions for future research, and clinical implications of the findings are also discussed.

  10. Enthusiastic portrayal of 3D bioprinting in the media: Ethical side effects.

    Science.gov (United States)

    Gilbert, Frederic; Viaña, John Noel M; O'Connell, Cathal D; Dodds, Susan

    2017-11-24

    There has been a surge in mass media reports extolling the potential for using three-dimensional printing of biomaterials (3D bioprinting) to treat a wide range of clinical conditions. Given that mass media is recognized as one of the most important sources of health and medical information for the general public, especially prospective patients, we report and discuss the ethical consequences of coverage of 3D bioprinting in the media. First, we illustrate how positive mass media narratives of a similar biofabricated technology, namely the Macchiarini scaffold tracheas, which was involved in lethal experimental human trials, influenced potential patient perceptions. Second, we report and analyze the positively biased and enthusiastic portrayal of 3D bioprinting in mass media. Third, we examine the lack of regulation and absence of discussion about risks associated with bioprinting technology. Fourth, we explore how media misunderstanding is dangerously misleading the narrative about the technology. © 2017 John Wiley & Sons Ltd.

  11. Representing Migration: Analysis of Media Portrayals of Syrian Migrant Women in Turkey

    Directory of Open Access Journals (Sweden)

    Ruhdan Uzun

    2017-06-01

    Full Text Available The media coverage of women migrations has played an important role in shaping public opinion and governmental policies. This article aims at exploring the media portrayals of Syrian Migrant Women in Turkey. For this purpose, the research data were collected from the three Turkish online newspaper websites, Hürriyet, Birgün and Sabah, each of which has different editorial policy in representing migration, during one year period. The findings of quantitative content analysis of the news stories regarding Syrian migrant women would provide an understanding of the gender dimension of migration in Turkey. It concludes that Syrian Migrant Women in Turkey generally are reported on crime news and are framed as victims or perpetrators. However, the newspapers rarely report their hard living conditions.

  12. Predictive Structure and Topology of Peroxisomal ATP-Binding Cassette (ABC Transporters

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    Pierre Andreoletti

    2017-07-01

    Full Text Available The peroxisomal ATP-binding Cassette (ABC transporters, which are called ABCD1, ABCD2 and ABCD3, are transmembrane proteins involved in the transport of various lipids that allow their degradation inside the organelle. Defective ABCD1 leads to the accumulation of very long-chain fatty acids and is associated with a complex and severe neurodegenerative disorder called X-linked adrenoleukodystrophy (X-ALD. Although the nucleotide-binding domain is highly conserved and characterized within the ABC transporters family, solid data are missing for the transmembrane domain (TMD of ABCD proteins. The lack of a clear consensus on the secondary and tertiary structure of the TMDs weakens any structure-function hypothesis based on the very diverse ABCD1 mutations found in X-ALD patients. Therefore, we first reinvestigated thoroughly the structure-function data available and performed refined alignments of ABCD protein sequences. Based on the 2.85  Å resolution crystal structure of the mitochondrial ABC transporter ABCB10, here we propose a structural model of peroxisomal ABCD proteins that specifies the position of the transmembrane and coupling helices, and highlight functional motifs and putative important amino acid residues.

  13. Phylogenetic analysis of the ATP-binding cassette transporter family in three mosquito species.

    Science.gov (United States)

    Lu, Hong; Xu, Yongyu; Cui, Feng

    2016-09-01

    The ATP-binding cassette (ABC) transporter family functions in the ATP-dependent transportation of various substrates across biological membranes. ABC proteins participate in various biological processes and insecticide resistance in insects, and are divided into eight subfamilies (A-H). Mosquitoes are important vectors of human diseases, but the mechanism by which the ABC transporter family evolves in mosquitoes is unknown. In this study, we classified and compared the ABC transporter families of three mosquitoes, namely, Anopheles gambiae, Aedes aegypti, and Culex pipiens quinquefasciatus. The three mosquitoes have 55, 69, and 70 ABC genes, respectively. The C. p. quinquefasciatus had approximately 40% and 65% expansion in the ABCG subfamily, mainly in ABCG1/G4, compared with the two other mosquito species. The ABCB, ABCD, ABCE, and ABCF subfamilies were conserved in the three mosquito species. The C. p. quinquefasciatus transcriptomes during development showed that the ABCG and ABCC genes were mainly highly expressed at the egg and pupal stages. The pigment-transport relative brown, white, and scarlet, as well as the ABCF subfamily, were highly expressed at the egg stage. The highly expressed genes in larvae included three ABCA3 genes. The majority of the highly expressed genes in adults were ABCG1/4 genes. These results provided insights into the evolution of the ABC transporter family in mosquitoes. Copyright © 2015 Elsevier B.V. All rights reserved.

  14. Additional cassettes for epitope and fluorescent fusion proteins in Candida albicans.

    Science.gov (United States)

    Gerami-Nejad, Maryam; Dulmage, Keely; Berman, Judith

    2009-07-01

    Epitope tags that confer specific properties, including affinity for resins or antibodies or detection by fluorescence microscopy, are highly useful for biochemical and cell biological investigations. In Candida albicans and several other related yeasts, the CUG codon specifies serine instead of leucine, requiring that molecular tools be customized for use in this important human fungal pathogen. Here we report the construction of a set of plasmids containing 13-Myc, 3HA, GST, V5 or His9 epitope cassettes that facilitate PCR-mediated construction of epitope-tagged proteins. Common primer sets amplify the different tags with two different selectable markers. In addition, we report construction of a codon-optimized Discosoma red fluorescent protein (DsRFP) gene. Like mCherryRFP, this DsRFP signal is detectable in transformants at the colony level and is useful in double-labelling experiments with green fluorescent protein (GFP). Finally, we describe a construct that directs PCR-mediated two-step insertion of GFP internal to a coding sequence, which facilitates tagging of secreted proteins, including GPI-anchor cell wall proteins that require endogenous N- and C-termini for function. These reagents expand the repertoire of molecular tools available for working with C. albicans and other members of the CUG clade of pathogenic yeasts.

  15. Expression of ATP-binding cassette membrane transporters in a HIV-1 transgenic rat model.

    Science.gov (United States)

    Robillard, Kevin R; Hoque, Md Tozammel; Bendayan, Reina

    2014-02-21

    P-glycoprotein (P-gp, product of Mdr1a and Mdr1b genes), multidrug resistance associated proteins (Mrps), and breast cancer resistance protein (Bcrp), all members of the ATP-binding cassette (ABC) membrane-associated drug transporters superfamily, can significantly restrict the entry of antiretroviral drugs (ARVs) into organs which exhibit a barrier function such as the central nervous system (CNS) and the male genital tract (MGT). In vitro, HIV-1 viral proteins such as glycoprotein-120 (gp120) and transcriptional transactivator (tat) have been shown to alter the expression of these transporters and ARVs permeability. The objective of this study was to compare mRNA expression of these transporters, in vivo, in several tissues obtained from HIV-1 transgenic rats (Tg-rat) (8 and 24 weeks) with those of age-matched wild-type rats. At 24 weeks, significant changes in several drug transporter mRNA expressions were observed, in particular, in brain, kidney, liver and testes. These findings suggest that HIV-1 viral proteins can alter the expression of ABC drug transporters, in vivo, in the context of HIV-1 and further regulate ARVs permeability in several organs including the CNS and MGT, two sites which have been reported to display very low ARVs permeability in the clinic. Copyright © 2014 Elsevier Inc. All rights reserved.

  16. Autonomous bioluminescent expression of the bacterial luciferase gene cassette (lux) in a mammalian cell line.

    Science.gov (United States)

    Close, Dan M; Patterson, Stacey S; Ripp, Steven; Baek, Seung J; Sanseverino, John; Sayler, Gary S

    2010-08-27

    The bacterial luciferase (lux) gene cassette consists of five genes (luxCDABE) whose protein products synergistically generate bioluminescent light signals exclusive of supplementary substrate additions or exogenous manipulations. Historically expressible only in prokaryotes, the lux operon was re-synthesized through a process of multi-bicistronic, codon-optimization to demonstrate for the first time self-directed bioluminescence emission in a mammalian HEK293 cell line in vitro and in vivo. Autonomous in vitro light production was shown to be 12-fold greater than the observable background associated with untransfected control cells. The availability of reduced riboflavin phosphate (FMNH(2)) was identified as the limiting bioluminescence substrate in the mammalian cell environment even after the addition of a constitutively expressed flavin reductase gene (frp) from Vibrio harveyi. FMNH(2) supplementation led to a 151-fold increase in bioluminescence in cells expressing mammalian codon-optimized luxCDE and frp genes. When injected subcutaneously into nude mice, in vivo optical imaging permitted near instantaneous light detection that persisted independently for the 60 min length of the assay with negligible background. The speed, longevity, and self-sufficiency of lux expression in the mammalian cellular environment provides a viable and powerful alternative for real-time target visualization not currently offered by existing bioluminescent and fluorescent imaging technologies.

  17. Autonomous bioluminescent expression of the bacterial luciferase gene cassette (lux in a mammalian cell line.

    Directory of Open Access Journals (Sweden)

    Dan M Close

    Full Text Available The bacterial luciferase (lux gene cassette consists of five genes (luxCDABE whose protein products synergistically generate bioluminescent light signals exclusive of supplementary substrate additions or exogenous manipulations. Historically expressible only in prokaryotes, the lux operon was re-synthesized through a process of multi-bicistronic, codon-optimization to demonstrate for the first time self-directed bioluminescence emission in a mammalian HEK293 cell line in vitro and in vivo.Autonomous in vitro light production was shown to be 12-fold greater than the observable background associated with untransfected control cells. The availability of reduced riboflavin phosphate (FMNH(2 was identified as the limiting bioluminescence substrate in the mammalian cell environment even after the addition of a constitutively expressed flavin reductase gene (frp from Vibrio harveyi. FMNH(2 supplementation led to a 151-fold increase in bioluminescence in cells expressing mammalian codon-optimized luxCDE and frp genes. When injected subcutaneously into nude mice, in vivo optical imaging permitted near instantaneous light detection that persisted independently for the 60 min length of the assay with negligible background.The speed, longevity, and self-sufficiency of lux expression in the mammalian cellular environment provides a viable and powerful alternative for real-time target visualization not currently offered by existing bioluminescent and fluorescent imaging technologies.

  18. Integrating retroviral cassette extends gene delivery of HSV-1 expression vectors to dividing cells.

    Science.gov (United States)

    de Felipe, P; Izquierdo, M; Wandosell, F; Lim, F

    2001-08-01

    Retroviral vectors have long been used in a wide variety of gene transfer applications but have certain drawbacks, such as small cargo size, limited tropism, and low titers. HSV expression vectors overcome these disadvantages, but, because they persist in target cells as nonreplicative episomes, they are not retained in all the progeny of dividing cells. Chimeric HSV/AAV products that can mediate transgene integration in human mitotic cells have been constructed, but, to date, genetic modification of dividing cells in animal models using HSV products has not been possible. Here, we report the construction of hybrid HSV/retroviral vectors that exhibit up to 50-fold higher transgene integration efficiency compared to vectors containing only HSV-1 components. Efficient integration of a retroviral transgene cassette encoding pac in human cells required expression of the Moloney murine leukemia virus gag-pol genes, but in murine cells, could also be mediated by endogenous activities, albeit at a lower level. Gene delivery was equally efficient in BHK21, a cell line resistant to retroviral infection, and transgene retention and expression were observed to be stable for least one month in Hs683 human glioma cells. These vectors have wide applications for the genetic modification of many cell types.

  19. Luminescence resonance energy transfer spectroscopy of ATP-binding cassette proteins.

    Science.gov (United States)

    Zoghbi, Maria E; Altenberg, Guillermo A

    2018-04-01

    The ATP-binding cassette (ABC) superfamily includes regulatory and transport proteins. Most human ABC exporters pump substrates out of cells using energy from ATP hydrolysis. Although major advances have been made toward understanding the molecular mechanism of ABC exporters, there are still many issues unresolved. During the last few years, luminescence resonance energy transfer has been used to detect conformational changes in real time, with atomic resolution, in isolated ABC nucleotide binding domains (NBDs) and full-length ABC exporters. NBDs are particularly interesting because they provide the power stroke for substrate transport. Luminescence resonance energy transfer (LRET) is a spectroscopic technique that can provide dynamic information with atomic-resolution of protein conformational changes under physiological conditions. Using LRET, it has been shown that NBD dimerization, a critical step in ABC proteins catalytic cycle, requires binding of ATP to two nucleotide binding sites. However, hydrolysis at just one of the sites can drive dissociation of the NBD dimer. It was also found that the NBDs of the bacterial ABC exporter MsbA reconstituted in a lipid bilayer membrane and studied at 37°C never separate as much as suggested by crystal structures. This observation stresses the importance of performing structural/functional studies of ABC exporters under physiologic conditions. This article is part of a Special Issue entitled: Beyond the Structure-Function Horizon of Membrane Proteins edited by Ute Hellmich, Rupak Doshi and Benjamin McIlwain. Copyright © 2017 Elsevier B.V. All rights reserved.

  20. ATP-binding cassette (ABC) proteins in aquatic invertebrates: Evolutionary significance and application in marine ecotoxicology.

    Science.gov (United States)

    Jeong, Chang-Bum; Kim, Hui-Su; Kang, Hye-Min; Lee, Jae-Seong

    2017-04-01

    The ATP-binding cassette (ABC) protein superfamily is known to play a fundamental role in biological processes and is highly conserved across animal taxa. The ABC proteins function as active transporters for multiple substrates across the cellular membrane by ATP hydrolysis. As this superfamily is derived from a common ancestor, ABC genes have evolved via lineage-specific duplications through the process of adaptation. In this review, we summarized information about the ABC gene families in aquatic invertebrates, considering their evolution and putative functions in defense mechanisms. Phylogenetic analysis was conducted to examine the evolutionary significance of ABC gene families in aquatic invertebrates. Particularly, a massive expansion of multixenobiotic resistance (MXR)-mediated efflux transporters was identified in the absence of the ABCG2 (BCRP) gene in Ecdysozoa and Platyzoa, suggesting that a loss of Abcg2 gene occurred sporadically in these species during divergence of Protostome to Lophotrochozoa. Furthermore, in aquatic invertebrates, the ecotoxicological significance of MXR is discussed while considering the role of MXR-mediated efflux transporters in response to various environmental pollutants. Copyright © 2017 Elsevier B.V. All rights reserved.

  1. ATP-binding cassette systems in Burkholderia pseudomallei and Burkholderia mallei

    Directory of Open Access Journals (Sweden)

    Titball Richard W

    2007-03-01

    Full Text Available Abstract Background ATP binding cassette (ABC systems are responsible for the import and export of a wide variety of molecules across cell membranes and comprise one of largest protein superfamilies found in prokarya, eukarya and archea. ABC systems play important roles in bacterial lifestyle, virulence and survival. In this study, an inventory of the ABC systems of Burkholderia pseudomallei strain K96243 and Burkholderia mallei strain ATCC 23344 has been compiled using bioinformatic techniques. Results The ABC systems in the genomes of B. pseudomallei and B. mallei have been reannotated and subsequently compared. Differences in the number and types of encoded ABC systems in belonging to these organisms have been identified. For example, ABC systems involved in iron acquisition appear to be correlated with differences in genome size and lifestyles between these two closely related organisms. Conclusion The availability of complete inventories of the ABC systems in B. pseudomallei and B. mallei has enabled a more detailed comparison of the encoded proteins in this family. This has resulted in the identification of ABC systems which may play key roles in the different lifestyles and pathogenic properties of these two bacteria. This information has the potential to be exploited for improved clinical identification of these organisms as well as in the development of new vaccines and therapeutics targeted against the diseases caused by these organisms.

  2. Molecular Characterization of LjABCG1, an ATP-Binding Cassette Protein in Lotus japonicus.

    Directory of Open Access Journals (Sweden)

    Akifumi Sugiyama

    Full Text Available LjABCG1, a full-size ABCG subfamily of ATP-binding cassette proteins of a model legume, Lotus japonicus, was reported as a gene highly expressed during the early stages of nodulation, but have not been characterized in detail. In this study we showed that the induction of LjABCG1 expression was remarkable by methyl jasmonate treatment, and reporter gene experiments indicated that LjABCG1 was strongly expressed in the nodule parenchyma and cell layers adjacent to the root vascular tissue toward the nodule. LjABCG1 was suggested to be localized at the plasma membrane based on the fractionation of microsomal membranes as well as separation via aqueous two-phase partitioning. The physiological functions of LjABCG1 in symbiosis and pathogenesis were analyzed in homologous and heterologous systems. LjABCG1 knock-down L. japonicus plants did not show clear phenotypic differences in nodule formation, and not in defense against Pseudomonas syringae, either. In contrast, when LjABCG1 was expressed in the Arabidopsis pdr8-1 mutant, the penetration frequency of Phytophthora infestans, a potato late blight pathogen, was significantly reduced in LjABCG1/pdr8-1 than in pdr8-1 plants. This finding indicated that LjABCG1, at least partially, complemented the phenotype of pdr8 in Arabidopsis, suggesting the multiple roles of this protein in plant-microbe interactions.

  3. ATP-binding cassette transporters in tumor endothelial cells and resistance to metronomic chemotherapy.

    Science.gov (United States)

    Hida, Kyoko; Kikuchi, Hiroshi; Maishi, Nako; Hida, Yasuhiro

    2017-08-01

    Drug resistance is a major problem in anticancer therapy. ATP-binding cassette (ABC) transporters have a role in the multidrug resistance. A new regimen of chemotherapy has been proposed, called "metronomic chemotherapy". Metronomic chemotherapy is the frequent, regular administration of drug doses designed to maintain low, but active, concentrations of chemotherapeutic drugs over prolonged periods of time, without causing serious toxicities. Metronomic chemotherapy regimens were developed to optimize the antitumor efficacy of agents that target the tumor vasculature instead of tumor cells, and to reduce toxicity of antineoplastic drugs [1]. Nevertheless, recent studies revealed that ABC transporters are expressed at a higher level in the endothelium in the tumor. To avoid resistance to metronomic anti-angiogenic chemotherapy, ABC transporter inhibition of tumor endothelial cells may be a promising strategy. In this mini-review, we discuss the possible mechanism of resistance to metronomic chemotherapy from the viewpoint of tumor endothelial cell biology, focusing on ABC transporters. Copyright © 2017. Published by Elsevier B.V.

  4. Melanin binding study of clinical drugs with cassette dosing and rapid equilibrium dialysis inserts.

    Science.gov (United States)

    Pelkonen, Laura; Tengvall-Unadike, Unni; Ruponen, Marika; Kidron, Heidi; Del Amo, Eva M; Reinisalo, Mika; Urtti, Arto

    2017-11-15

    Melanin pigment is a negatively charged polymer found in pigmented human tissues. In the eye, iris, ciliary body, choroid and retinal pigment epithelium (RPE) are heavily pigmented. Several drug molecules are known to bind to melanin, but larger sets of drugs have not been compared often in similar test conditions. In this study, we introduce a powerful tool for screening of melanin binding. The binding of a set of 34 compounds to isolated porcine RPE melanin was determined by cassette (n-in-one) dosing in rapid equilibrium dialysis inserts and the binding was quantitated with LC-MS/MS analytics. The compounds represented large variety in melanin binding (from 8.6%, ganciclovir) to over 95% bound (ampicillin and ciprofloxacin). The data provides information on melanin binding of small molecular weight compounds that are used for ocular (e.g. brinzolamide, ganciclovir) and systemic (e.g. tizanidine, indomethacin) therapy. Interestingly, competition among compounds was seen for melanin binding and the binding did not show any correlation with plasma protein binding. These results increase the understanding of melanin binding of ocular drugs and can be further exploited to predict pharmacokinetics in the eye. Pigment binding provides an interesting option for improved drug distribution to retina and choroid that are difficult target tissues in drug delivery. Copyright © 2017 Elsevier B.V. All rights reserved.

  5. Role of pharmacogenetics of ATP-binding cassette transporters in the pharmacokinetics of drugs.

    Science.gov (United States)

    Cascorbi, Ingolf

    2006-11-01

    Interindividual differences of drug response are an important cause of treatment failures and adverse drug reactions. The identification of polymorphisms explaining distinct phenotypes of drug metabolizing enzymes contributed in part to the understanding of individual variations of drug plasma levels. However, bioavailability also depends on a major extent from the expression and activity of drug transport across biomembranes. In particular efflux transporters of the ATP-binding cassette (ABC) family such as ABCB1 (P-glycoprotein, P-gp), the ABCC (multidrug resistance-related protein, MRP) family and ABCG2 (breast cancer resistance protein, BCRP) have been identified as major determinants of chemoresistance in tumor cells. They are expressed in the apical membranes of many barrier tissue such as the intestine, liver, blood-brain barrier, kidney, placenta, testis and in lymphocytes, thus contributing to plasma, liquor, but also intracellular drug disposition. Since expression and function exhibit a broad variability, it was hypothesized that hereditary variances in the genes of membrane transporters could explain at least in part interindividual differences of pharmacokinetics and clinical outcome of a variety of drugs. This review focuses on the functional significance of single nucleotide polymorphisms (SNP) of ABCB1, ABCC1, ABCC2, and ABCG2 in in vitro systems, in vivo tissues and drug disposition, as well as on the clinical outcome of major indications.

  6. Energy use of televisions and video cassette recorders in the U.S.

    Energy Technology Data Exchange (ETDEWEB)

    Meier, Alan; Rosen, Karen

    1999-03-01

    In an effort to more accurately determine nationwide energy consumption, the U.S. Department of Energy has recently commissioned studies with the goal of improving its understanding of the energy use of appliances in the miscellaneous end-use category. This study presents an estimate of the residential energy consumption of two of the most common domestic appliances in the miscellaneous end-use category: color televisions (TVs) and video cassette recorders (VCRs). The authors used a bottom-up approach in estimating national TV and VCR energy consumption. First, they obtained estimates of stock and usage from national surveys, while TV and VCR power measurements and other data were recorded at repair and retail shops. Industry-supplied shipment and sales distributions were then used to minimize bias in the power measurement samples. To estimate national TV and VCR energy consumption values, ranges of power draw and mode usage were created to represent situations in homes with more than one unit. Average energy use values for homes with one unit, two units, etc. were calculated and summed to provide estimates of total national TV and VCR energy consumption.

  7. Divergent action of calcium channel blockers on ATP-binding cassette protein expression.

    Science.gov (United States)

    Hasegawa, Kazuhiro; Wakino, Shu; Kanda, Takeshi; Yoshioka, Kyoko; Tatematsu, Satoru; Homma, Koichiro; Takamatsu, Ichiro; Sugano, Naoki; Hayashi, Koichi

    2005-12-01

    Calcium channel blockers (CCBs) are widely used in clinical practice, and have been reported to be effective in preventing the progression of atherosclerosis. We examined whether various types of calcium channel blockers affected the expression of ATP binding cassette transporter A1 (ABCA1), a factor contributing to anti-atherogenesis. Undifferentiated monocytic cell line, THP-1 cells were maintained in RPMI 1640 medium and treated with different kinds of calcium channel blockers. Among the calcium channel blockers tested, aranidipine and efonidipine increased ABCA1 protein expression without an increase in ABCA1 mRNA expression, whereas other calcium channel blockers (eg, nifedipine, amlodipine, and nicardipine) or T-type calcium channel blockers (eg, mibefradil and nickel chloride) failed to upregulate ABCA1 expression. H89, a protein kinase A inhibitor inhibited the aranidipine-induced ABCA1 protein expression, whereas genistein (a tyrosine kinase inhibitor), or AG490 (a JAK-2 inhibitor) had no effects. Neither of these inhibitors suppressed the efonidipine-induced ABCA1 protein expression. Intracellular cAMP levels were elevated only by aranidipine, but not by efonidipine. In conclusion, aranidipine and efonidipine have the ability to induce ABCA1 protein by distinct mechanisms; protein kinase A is involved in the aranidipine-induced ABCA1 upregulation. This non-class effect of calcium channel blockers may potentially offer beneficial action in the treatment of hypertensive subjects with atherosclerosis.

  8. Based on a True Story? The Portrayal of ECT in International Movies and Television Programs.

    Science.gov (United States)

    Sienaert, Pascal

    Movies and television (TV) programs are an important source of public information about ECT. To narratively review the portrayal of ECT in international movies and TV programs from 1948 until present. Several Internet movie databases and a database of phrases appearing in movies and TV programs were searched, supplemented with a Medline-search. No language restrictions were applied. ECT was portrayed in 52 movies (57 scenes), 21 TV programs (23 scenes), and 2 animated sitcoms (2 scenes). In movies, the main indication for ECT is behavioral control or torture (17/57, 29.8%), whereas in TV programs, the most frequent indication is erasing memories (7/25, 28%). In most scenes (47/82; 57.3%) ECT is given without consent, and without anesthesia (59/82; 72%). Unmodified ECT is depicted more frequently in American scenes (48/64, 75%), as opposed to scenes from other countries (11/18; 64.7%). Bilateral electrode placement is used in almost all (89%, 73/82) scenes. The vast majority of movies (46/57, 80.7%) and TV programs (18/25, 72%) show a negative and inaccurate image of the treatment. In the majority of scenes, ECT is used as a metaphor for repression, mind and behavior control, and is shown as a memory-erasing, painful and damaging treatment, adding to the stigma already associated with ECT. Only a few exceptions paint a truthful picture of this indispensable treatment in modern psychiatry. Copyright © 2016 Elsevier Inc. All rights reserved.

  9. The portrayal of mental health and illness in Australian non-fiction media.

    Science.gov (United States)

    Francis, Catherine; Pirkis, Jane; Blood, R Warwick; Dunt, David; Burgess, Philip; Morley, Belinda; Stewart, Andrew; Putnis, Peter

    2004-07-01

    To provide a detailed picture of the extent, nature and quality of portrayal of mental health/illness in Australian non-fiction media. Media items were retrieved from Australian newspaper, television and radio sources over a 1-year period, and identifying/descriptive data extracted from all items. Quality ratings were made on a randomly selected 10% of items, using an instrument based on criteria in Achieving the Balance (a resource designed to promote responsible reporting of mental health/illness). Reporting of mental health/illness was common, with 4351 newspaper, 1237 television and 7801 radio items collected during the study period. Media items most frequently focused on policy/program initiatives in mental health (29.0%), or on causes/symptoms/treatment of mental illnesses (23.9%). Stories about mental health issues in the context of crime were relatively uncommon, accounting for only 5.6% of items. Most media items were of good quality on eight of the nine dimensions; the exception was that details of appropriate help services were only included in 6.4% of items. In contrast to previous research, the current study found that media reporting of mental health/illness was extensive, generally of good quality and focused less on themes of crime and violence than may have been expected. This is encouraging, since there is evidence that negative media portrayal of mental health/illness can detrimentally affect community attitudes. However, there are still opportunities for improving media reporting of mental health/illness, which should be taken up in future media strategies.

  10. Violent film characters' portrayal of alcohol, sex, and tobacco-related behaviors.

    Science.gov (United States)

    Bleakley, Amy; Romer, Daniel; Jamieson, Patrick E

    2014-01-01

    To determine the extent to which movies popular with adolescents feature characters who jointly engage in violence and other risk behaviors. We hypothesized that violent characters engage in other risk behaviors equally often in films rated appropriate for children over 12 (PG-13) and Restricted (R)-rated films. Content analysis of a sample of top-grossing movies from 1985 to 2010 (n = 390). We coded movies for the presence of at least 1 main character who was involved in violence and either sex, tobacco, or alcohol use within a 5-minute movie segment and throughout a film. Approximately 90% of the movies contained a segment with a main character involved in violence, and ~77% of the films had the same character engaging in at least 1 other risk behavior. A violent character was portrayed most often partaking in alcohol-related and sexual behaviors. G and PG movies had less co-occurrence than PG-13 or R-rated movies, but there was no statistical difference between PG-13 and R-rated movies with regards to violence co-occurring with other risk behaviors. These trends did not vary over time. Popular films that contain violent characters also show those characters engaging in other risk behaviors. Similar rates of co-occurrence between PG-13 and R-rated films suggest that the Motion Picture Association of America ratings system is not sensitive to the joint portrayal of violence and alcohol, sex, and tobacco-related risk behaviors. The on-screen clustering of violence with other risk behaviors is cause for concern and worthy of additional research.

  11. Sympathy, shame, and few solutions: News media portrayals of fetal alcohol spectrum disorders.

    Science.gov (United States)

    Eguiagaray, Ines; Scholz, Brett; Giorgi, Caterina

    2016-09-01

    there is a lack of public understanding about fetal alcohol spectrum disorders (FASD), and many countries lack policies to deal with FASD concerns. Given the role of news media in disseminating a range of health information, the aim of the current study was to explore the media coverage on alcohol use during pregnancy and FASD, and to identify ways to improve associated health messages. the current study uses a framing analysis of news media reports about FASD over a 1-year period. Framing analysis seeks to better understand how media messages serve to shape the thoughts, feelings, and decisions of readers. two frames dominated the media coverage of FASD: a frame of sympathy, and a frame of shame. Some news media encouraged feelings of sympathy for children with FASD, while others encouraged sympathy towards mothers of these children. At the same time, mothers were also portrayed as deserving of shame. the interrelated frames of sympathy and shame may confuse readers, as they inconsistently hold different parties responsible for the impact of FASD. Media portrayals that encourage women to refrain from alcohol consumption during pregnancy might be more useful than stigmatising and isolating those who do. practitioners should be aware that conflicting messages about alcohol consumption during pregnancy might lead to shame and confusion, and should encourage openness with mothers to challenge stigma. Guidelines for media reporting should discourage stigmatising frames, and media articles should also consider the role that government, non-government organisations, and the alcohol industry could play for improving FASD shame. Copyright © 2016 Elsevier Ltd. All rights reserved.

  12. "Bouncing back": how Australia's leading women's magazines portray the postpartum 'body'.

    Science.gov (United States)

    Roth, Heike; Homer, Caroline; Fenwick, Jennifer

    2012-09-01

    To examine how the Australian media portrays the childbearing body through the use of celebrity stories in women's magazines. The study aimed to provide insight into socially constructed factors that might influence women's body image and expectations during pregnancy and the early postnatal period. Media content analysis was used to analyse 25 celebrity stories about the childbearing postnatal body (images and texts) collected from Australia's three leading women's magazines between January and June 2009 (n=58). A variety of persuasive textual and visual messages were elicited. The major theme representing how the postnatal body was constructed was labelled 'Bouncing back'; the focus of this paper. The social messages inherent in the magazine stories were that women need to strive towards regaining a pre-pregnant body shape with the same effort one would employ when recovering from an illness. Three specific sub-themes that promoted weight loss were identified. These were labelled 'Racing to bounce back', 'Breastfeeding to bounce back' and 'Pretending to bounce back'. A fourth sub-theme, 'Refusing to bounce back: Celebrating my new body', grouped together stories about celebrities who appeared to embrace their changed, but healthy, postnatal body. The study highlighted the expectations of the postpartum body in relation to speedy return to the pre-pregnant state. Understanding how these portrayals may contribute to women's own body image and expectations in the early postpartum period may better assist maternity health care providers to engage with women in meaningful discussions about this important time in their lives and challenge notions of ideal body types. Assisting women to accept and nurture themselves and have confidence in their ability as a new parent is a crucial element of quality maternity service provision. Copyright © 2011 Australian College of Midwives. Published by Elsevier Ltd. All rights reserved.

  13. Portrayal of Alcohol Brands Popular Among Underage Youth on YouTube: A Content Analysis.

    Science.gov (United States)

    Primack, Brian A; Colditz, Jason B; Rosen, Eva B; Giles, Leila M; Jackson, Kristina M; Kraemer, Kevin L

    2017-09-01

    We characterized leading YouTube videos featuring alcohol brand references and examined video characteristics associated with each brand and video category. We systematically captured the 137 most relevant and popular videos on YouTube portraying alcohol brands that are popular among underage youth. We used an iterative process to codebook development. We coded variables within domains of video type, character sociodemographics, production quality, and negative and positive associations with alcohol use. All variables were double coded, and Cohen's kappa was greater than .80 for all variables except age, which was eliminated. There were 96,860,936 combined views for all videos. The most common video type was "traditional advertisements," which comprised 40% of videos. Of the videos, 20% were "guides" and 10% focused on chugging a bottle of distilled spirits. While 95% of videos featured males, 40% featured females. Alcohol intoxication was present in 19% of videos. Aggression, addiction, and injuries were uncommonly identified (2%, 3%, and 4%, respectively), but 47% of videos contained humor. Traditional advertisements represented the majority of videos related to Bud Light (83%) but only 18% of Grey Goose and 8% of Hennessy videos. Intoxication was most present in chugging demonstrations (77%), whereas addiction was only portrayed in music videos (22%). Videos containing humor ranged from 11% for music-related videos to 77% for traditional advertisements. YouTube videos depicting the alcohol brands favored by underage youth are heavily viewed, and the majority are traditional or narrative advertisements. Understanding characteristics associated with different brands and video categories may aid in intervention development.

  14. Good News? A Longitudinal Analysis of Newspaper Portrayals of Mental Illness in Canada 2005 to 2015.

    Science.gov (United States)

    Whitley, Rob; Wang, JiaWei

    2017-04-01

    The overarching aim of this article is to assess media portrayals of mental illness in Canada. We hypothesise that portrayals have improved over time, related to the various antistigma activities of organisations such as the Mental Health Commission of Canada (MHCC). Specific objectives are to assess 1) overall tone and content of newspaper articles, 2) change over time, and 3) variables associated with positive or negative content. We collected newspaper articles from print and online editions of over 20 best-selling Canadian newspapers from 2005 to 2015 ( N = 24,570) that mentioned key search terms such as mental illness or schizophrenia. These were read by research assistants, who assessed tone and content for each article using preassigned codes and categories. Data were subjected to chi-squared and trend analysis. Over the study period, 21% of the articles had a positive tone and 28% had stigmatising content. Trend analysis suggested significantly improved coverage over 11 years ( P < 0.001). For example, articles with a positive tone had almost doubled from 2005 (18.9%) to 2015 (34.8%), and articles with stigmatising content had reduced by a third (22.3% vs 32.7%). Analysis also suggested that articles on the front page, as well as articles in broadsheet newspapers, had significantly more positive coverage. The study indicates that news media coverage related to mental illness has improved over the past decade. This may be related to the concerted efforts of the MHCC, which has executed a targeted strategy aimed at reducing stigma and improving media coverage since 2007.

  15. Plasmodium falciparum erythrocyte membrane protein 1 domain cassettes 8 and 13 are associated with severe malaria in children

    DEFF Research Database (Denmark)

    Lavstsen, Thomas; Turner, Louise; Saguti, Fredy

    2012-01-01

    The clinical outcome of Plasmodium falciparum infections ranges from asymptomatic parasitemia to severe malaria syndromes associated with high mortality. The virulence of P. falciparum infections is associated with the type of P. falciparum erythrocyte membrane protein 1 (PfEMP1) expressed...... amplifying var subtype-specific loci covering most var/PfEMP1 subtypes. In addition, we characterized the near-full-length sequence of the most prominently expressed var genes in three patients diagnosed with severe anemia and/or cerebral malaria. The combined analysis showed that severe malaria syndromes......, including severe anemia and cerebral malaria, are associated with high transcript levels of PfEMP1 domain cassette 8-encoding var genes. Transcript levels of group A var genes, including genes encoding domain cassette 13, were also significantly higher in patients with severe syndromes compared with those...

  16. The role of ATP-binding cassette transporter A2 in childhood acute lymphoblastic leukemia multidrug resistance

    OpenAIRE

    Aberuyi, N; S. Rahgozar; Moafi, A

    2014-01-01

    Acute lymphoblastic leukemia (ALL) is one of the most prevalent hematologic malignancies in children. Although the cure rate of ALL has improved over the past decades, the most important reason for ALL treatment failure is multidrug resistance (MDR) phenomenon. The current study aims to explain the mechanisms involved in multidrug resistance of childhood ALL, and introduces ATP-binding cassette transporterA2 (ABCA2) as an ABC transporter gene which may have a high impact on MDR. Benefiting fr...

  17. Characteriz ation of integrons and associated gene cassettes in Acinetobacter baumannii strains isolated from intensive care unit in Tehran, Iran

    Directory of Open Access Journals (Sweden)

    Hossein Goudarzi

    2016-09-01

    Full Text Available Objective: To determine the antimicrobial susceptibility patterns, the frequency of integrons and associated gene cassettes in Acinetobacter baumannii (A. baumannii strains isolated from selected hospital intensive care units. Methods: During a ten-month period, 120 A. baumannii isolates were studied. The resistance rates to different classes of antimicrobial agents were determined. PCR was used to detect different types of integrons and associated gene cassettes. Results: The resistance rates to the majority of antibiotics tested were found to be between 39.3% and 99.1%. No isolate was observed to be resistant to colistin and polymyxin B. The rate of extensive drug-resistance among these clinical isolates was 62.5%. The prevalence of class 1 and 2 integrons was found to be 74.1% and 12.5%, respectively. Seven different gene cassettes (ampC, aacA4-catB8, ISAba1-blaOXA-23-GES-14, aadA2-cm1A6-GES-14-qacF, VIM-25-GES-24-qacF, dfrA5-ISAba1-blaOXA-51-blaOXA-40 and aadA2-GES-11-IMP-1 were observed in Class 1 integron-carrying strains. Three gene cassettes (IMP-4, VIM-2-VEB-aacA4 and dfrA2-sat-2-aadA4 were detected in class 2 integron-bearing A. baumannii strains. Conclusions: A high prevalence of integron was described among multidrug resistant A. baumannii in the hospital. The findings highlighted the need for continuous surveillance in order to prevent dissemination of multidrug resistance among A. baumannii strains in Iran.

  18. High-level expression from two independent expression cassettes in replication-incompetent adenovirus type 35 vector.

    Science.gov (United States)

    Vogels, Ronald; Zuijdgeest, David; van Meerendonk, Michelle; Companjen, Arjen; Gillissen, Gert; Sijtsma, Jeroen; Melis, Irene; Holterman, Lennart; Radosevic, Katarina; Goudsmit, Jaap; Havenga, Menzo J E

    2007-11-01

    Replication-incompetent adenovirus type 35 (rAd35) represents a potent vaccine carrier that elicits strong, antigen-specific T- and B-cell responses in diverse preclinical models. Moreover, Ad35 is rare in human populations, resulting in the absence of neutralizing antibodies against this carrier, in contrast to the commonly used rAd5. Therefore, rAd35 is being investigated as a vaccine carrier for a number of diseases for which an effective vaccine is needed, including malaria, AIDS and tuberculosis. However, it can be perceived that effective immunization will require insertion of multiple antigens into adenoviral vectors. We therefore wanted to create rAd35 vectors carrying double expression cassettes, to expand within one vector the number of insertion sites for foreign DNA encoding antigenic proteins. We show that it is possible to generate rAd35 vectors carrying two cytomegalovirus promoter-driven expression cassettes, provided that the polyadenylation signals in each expression cassette are not identical. We demonstrate excellent rAd35 vector stability and show that expression of a transgene is not influenced by the presence of a second expression cassette. Moreover, by using two model vaccine antigens, i.e. the human immunodeficiency virus-derived Env-gp120 protein and the Plasmodium falciparum-derived circumsporozoite protein, we demonstrate that potent T- and B-cell responses are induced to both antigens expressed from a single vector. Such rAd35 vectors thus expand the utility of rAd35 vaccine carriers for the development of vaccines against, for example, malaria, AIDS and tuberculosis.

  19. Computational prediction of CRISPR cassettes in gut metagenome samples from Chinese type-2 diabetic patients and healthy controls

    OpenAIRE

    Mangericao, Tatiana C.; Peng, Zhanhao; Zhang, Xuegong

    2016-01-01

    Background CRISPR has been becoming a hot topic as a powerful technique for genome editing for human and other higher organisms. The original CRISPR-Cas (Clustered Regularly Interspaced Short Palindromic Repeats coupled with CRISPR-associated proteins) is an important adaptive defence system for prokaryotes that provides resistance against invading elements such as viruses and plasmids. A CRISPR cassette contains short nucleotide sequences called spacers. These unique regions retain a history...

  20. Structural design of DEMO Divertor Cassette Body: provisional FEM analysis and introductive application of RCC-MRx design rules

    Energy Technology Data Exchange (ETDEWEB)

    Frosi, Paolo, E-mail: paolo.frosi@enea.it [Unità Tecnica Fusione-ENEA C.R. Frascati, Via E. Fermi 45, 00044 Frascati (Italy); Mazzone, Giuseppe [Unità Tecnica Fusione-ENEA C.R. Frascati, Via E. Fermi 45, 00044 Frascati (Italy); You, Jeong-Ha [Max Planck Institute of Plasma Physics, Boltzmann Str. 2, 85748 Garching (Germany)

    2016-11-01

    This paper deals with the early steps in developing a structural fem model of DEMO Divertor. The study is focused on the thermal and structural analysis of the Cassette Body: a new geometry has been developed for this component: it is foreseen that the plasma facing component (PFC) will be directly placed on the cassette but for the Dome no choice has been adopted yet. For now the model contains only a suitable schematization of the Cassette Body and its objective is to analyze the effect produced by the main loads (electromagnetic loads, coolant pressure, thermal neutron and convective loads) on itself: an available estimate of loads is that one derived from ITER: for a proper translation some assumptions have been made and they are described in the paper. Now it is not a primary purpose to obtain some definitive statements about stresses, displacements, temperatures and so on; the authors want to construct a set of FEM models that will help all the decisions of DEMO Divertor design in its future development. This set is conceived as a tool that shall be improved to account for all the main enhancements that will be found in geometry, in material properties data and in load evaluations. Moreover, the main design variables (loads, material properties, some geometric items, mesh element size) are defined as parameters. This work considers also an introductive approach for future structural verification of the Divertor Cassette Body: so a concern of the Design and Construction Rules for Mechanical Components of Nuclear Installation (RCC-MRx) has been implemented. The FEM code used is Ansys rel. 15.

  1. Emergence of a multidrug-resistant Citrobacter freundii ST8 harboring an unusual VIM-4 gene cassette in Poland

    Directory of Open Access Journals (Sweden)

    Piotr Majewski

    2017-08-01

    Conclusions: All unusual gene cassettes containing VIM-DR (direct repeat described thus far have been harbored by non-fermenters, i.e., Acinetobacter and Pseudomonas, underscoring the importance of resistance determinant mobility, which may go even beyond genus, family, and order boundaries. Great efforts need to be taken to explore pathways of resistance to ‘last-resort’ antimicrobials, especially among clinically relevant pathogens.

  2. Characterisation of class 3 integrons with oxacillinase gene cassettes in hospital sewage and sludge samples from France and Luxembourg.

    Science.gov (United States)

    Simo Tchuinte, Pierrette Landrie; Stalder, Thibault; Venditti, Silvia; Ngandjio, Antoinette; Dagot, Christophe; Ploy, Marie-Cécile; Barraud, Olivier

    2016-10-01

    In this study, antibiotic resistance class 3 integrons in Gram-negative bacteria isolated from hospital sewage and sludge and their genetic contents were characterised. Two samples of hospital effluent from France and Luxembourg and one sample of sludge from a wastewater treatment plant in France were collected in 2010 and 2011. Bacteria were cultured on selective agar plates and integrons were detected in colonies by quantitative PCR. Integron gene cassette arrays and their genetic environments were analysed by next-generation sequencing. Three class 3 integron-positive isolates were detected, including Acinetobacter johnsonii LIM75 (French hospital effluent), Aeromonas allosaccharophila LIM82 (sludge) and Citrobacter freundii LIM86 (Luxembourg hospital effluent). The gene cassettes were all implicated in antibiotic (aminoglycoside and β-lactam) or antiseptic resistance. An oxacillinase gene cassette (blaOXA-10, blaOXA-368 or blaOXA-2) was found in each integron. All of the class 3 integrons were located on small mobilisable plasmids. This study highlights the role of class 3 integrons in the dissemination of clinically relevant antibiotic resistance genes, notably oxacillinase genes, in hospital effluent. Copyright © 2016 Elsevier B.V. and International Society of Chemotherapy. All rights reserved.

  3. Molecular Events Involved in a Single Cycle of Ligand Transfer from an ATP Binding Cassette Transporter, LolCDE, to a Molecular Chaperone, LolA*

    OpenAIRE

    Taniguchi, Naohiro; Tokuda, Hajime

    2008-01-01

    An ATP binding cassette transporter LolCDE complex releases lipoproteins from the inner membrane of Escherichia coli in an ATP-dependent manner, leading to the formation of a complex between a lipoprotein and a periplasmic chaperone, LolA. LolA is proposed to undergo a conformational change upon the lipoprotein binding. The lipoprotein is then transferred from the LolA-lipoprotein complex to the outer membrane via LolB. Unlike most ATP binding cassette transporters med...

  4. Media Portrayals of Love, Marriage & Sexuality for Child Audiences: A Select Content Analysis of Walt Disney Animated Family Films.

    Science.gov (United States)

    Junn, Ellen N.

    This study examined the portrayal of love, marriage, and sexuality in 11 romantic and nonromantic Disney animated films. Results showed that four out of five of the nonromantic films had male leads, with males occupying significantly more screen time than females. Half of the romantic films had female leads, who occupied significantly more screen…

  5. Deconstructing the Portrayals of Haitian Women in the Media: A Thematic Analysis of Images in the Associated Press Photo Archive

    Science.gov (United States)

    Rendon, Maria Jose; Nicolas, Guerda

    2012-01-01

    Haitian women constitute a group that is lauded within Haiti as the "pillar of society" and yet is also often silenced both within Haiti and abroad. Given the role of the media in shaping attitudes and behaviors toward Women of Color, evaluation of media portrayals is critical to challenge oppressive discourses about these groups. Therefore, in…

  6. Folk Dress, Fiestas, and Festivals: How Is Mexico Portrayed in U.S. Primary Grade Social Studies Textbooks?

    Science.gov (United States)

    Field, Sherry L.; Bauml, Michelle; Wilhelm, Ron W.; Jenkins, Joelle

    2012-01-01

    This qualitative content analysis was conducted to determine how Mexico and Mexican people are portrayed in contemporary U.S. elementary social studies textbooks. Three textbooks from each of three major publishers were analyzed. Findings are presented in five sections: images and photographs, holidays, Mexican people and heroes, contested…

  7. Women on display: the effect of portraying the self online on women’s self-objectification

    NARCIS (Netherlands)

    de Vries, D.; Peter, J.

    2013-01-01

    Objectification research has largely ignored the potential impact of Internet activities, such as online self-portrayal, on women’s self-objectification and whether this may interact with traditional objectifying stimuli. In response to these research gaps, the present study had two goals: first, to

  8. Women on display: the effect of portraying the self online on women’s self-objectification

    NARCIS (Netherlands)

    de Vries, D.A.; Peter, J.

    2013-01-01

    Objectification research has largely ignored the potential impact of Internet activities, such as online self-portrayal, on women’s self-objectification and whether this may interact with traditional sexually objectifying stimuli. In response to these research gaps, the present study had two goals:

  9. Promotion of Waterpipe Tobacco Use, Its Variants and Accessories in Young Adult Newspapers: A Content Analysis of Message Portrayal

    Science.gov (United States)

    Sterling, Kymberle L.; Fryer, Craig S.; Majeed, Ban; Duong, Melissa M.

    2015-01-01

    The objective of our study was to identify waterpipe tobacco smoking advertisements and those that promoted a range of products and accessories used to smoke waterpipe tobacco. The content of these advertisements was analyzed to understand the messages portrayed about waterpipe tobacco smoking in young adult (aged 18-30) newspapers. The study…

  10. Alcohol portrayals in movies, music videos and soap operas and alcohol use of young people: current status and future challenges

    NARCIS (Netherlands)

    Koordeman, R.; Anschutz, D.J.; Engels, R.C.M.E.

    2012-01-01

    Aims: To provide an overview of studies of the effects of alcohol portrayals in movies, music videos and soap operas on alcohol consumption among young people. Moreover, we highlight important issues that need to be addressed in future research. Methods: This paper reviews the current literature on

  11. Alcohol portrayals in movies, music videos and soap operas and alcohol use of young people: Current status and future challenges

    NARCIS (Netherlands)

    Koordeman, R.; Anschutz, D.J.; Engels, R.C.M.E.

    2012-01-01

    Aims: To provide an overview of studies of the effects of alcohol portrayals in movies, music videos and soap operas on alcohol consumption among young people. Moreover, we highlight important issues that need to be addressed in future research. Methods: This paper reviews the current literature on

  12. The Portrayal of "Foreigners" in Japanese Social Studies Textbooks: Self-Images of Mono-Ethnic Pluralism

    Science.gov (United States)

    Tsuneyoshi, Ryoko

    2007-01-01

    History and social studies textbooks have often been the object of heated political debate in various countries, since they relate directly to issues of national identity and citizenship. This article analyzes how "foreigners" are portrayed in two versions (the 2000 and 2006 versions, date of issue) of the best-selling elementary social…

  13. Old Age Portrayed by the Ages-of-Life Models from the Middle Ages to the Sixteenth Century.

    Science.gov (United States)

    Covey, Herbert C.

    1989-01-01

    The ages of life portrayed in literature and the arts from the late Middle Ages up to the sixteenth century provide valuable information on how aging and old age were perceived in earlier times. Themes related to old age include ambivalence, decay, and age-appropriate behavior. (Author)

  14. Farmhands and Factory Workers, Honesty and Humility: The Portrayal of Social Class and Morals in English Language Learner Children's Books

    Science.gov (United States)

    Sano, Joelle

    2009-01-01

    Background/Context: Although much research has evaluated children's books for depictions of gender, little has centered on the portrayal of immigrants and social class. This investigation utilizes Bourdieu's theory of capital reproduction in education, Durkheim's conception of collective conscience and morals, and Bowles and Gintis's critique of…

  15. The framing of alcohol in (non-)sponsored movies: A comparative content analysis of alcohol advertising and portrayals in sponsored and non-sponsored Dutch movies

    NARCIS (Netherlands)

    Gosselt, Jordi Franciscus; van Hoof, Joris Jasper; Kokkeler, Liesbeth

    2017-01-01

    Aims: Alcohol advertising, in the form of product placement, has been shown to influence the viewer’s alcohol consumption. However, it is not just the portrayal itself that affects behavioural outcomes; the particular message that is conveyed in an alcohol portrayal may actually influence consumer

  16. Associating with Occupational Depictions: How African American College Women Are Influenced by the Portrayals of Women in Professional Careers on Television

    Science.gov (United States)

    Vanderlinden, Mary E.

    2011-01-01

    This study examined ways portrayals of professional Black women on television influence the higher education and occupational choices of African American college women. The central research question of this study was: How do college age African American women make meaning of the portrayals of the people they see on television? Two analytic…

  17. Filling a missing link: the influence of portrayals of older characters in television commercials on the memory performance of older adults

    NARCIS (Netherlands)

    Westerhof, Gerben Johan; Harink, Karolien; van Selm, Martine; Strick, Madelijn; van Baaren, Rick

    2010-01-01

    The portrayal of older characters in television commercials has over time become more varied and positive. This study examines how different portrayals of older characters relate to self-stereotyping, a process through which older individuals apply their beliefs about older people in general to

  18. The gRNA-miRNA-gRNA Ternary Cassette Combining CRISPR/Cas9 with RNAi Approach Strongly Inhibits Hepatitis B Virus Replication

    Science.gov (United States)

    Wang, Jie; Chen, Ran; Zhang, Ruiyang; Ding, Shanlong; Zhang, Tianying; Yuan, Quan; Guan, Guiwen; Chen, Xiangmei; Zhang, Ting; Zhuang, Hui; Nunes, Frederick; Block, Timothy; Liu, Shuang; Duan, Zhongping; Xia, Ningshao; Xu, Zhongwei; Lu, Fengmin

    2017-01-01

    The CRISPR/Cas9 system is a novel genome editing technology which has been successfully used to inhibit HBV replication. Here, we described a novel gRNA-microRNA (miRNA)-gRNA ternary cassette driven by a single U6 promoter. With an anti-HBV pri-miR31 mimic integrated between two HBV-specific gRNAs, both gRNAs could be separated from the long transcript of gRNA-miR-HBV-gRNA ternary cassette through Drosha/DGCR8 processing. The results showed that the gRNA-miR-HBV-gRNA ternary cassette could efficiently express two gRNAs and miR-HBV. The optimal length of pri-miRNA flanking sequence in our ternary cassette was determined to be 38 base pairs (bp). Besides, HBV-specific gRNAs and miR-HBV in gRNA-miR-HBV-gRNA ternary cassette could exert a synergistic effect in inhibiting HBV replication and destroying HBV genome in vitro and in vivo. Most importantly, together with RNA interference (RNAi) approach, the HBV-specific gRNAs showed the potent activity on the destruction of HBV covalently closed circular DNA (cccDNA). Since HBV cccDNA is an obstacle for the elimination of chronic HBV infection, the gRNA-miR-HBV-gRNA ternary cassette may be a potential tool for the clearance of HBV cccDNA. PMID:28839466

  19. Contamination of X-ray cassettes with methicillin-resistant Staphylococcus aureus and methicillin-resistant Staphylococcus haemolyticus in a radiology department.

    Science.gov (United States)

    Kim, Jae-Seok; Kim, Han-Sung; Park, Ji-Young; Koo, Hyun-Sook; Choi, Chul-Sun; Song, Wonkeun; Cho, Hyoun Chan; Lee, Kyu Man

    2012-05-01

    We performed surveillance cultures of the surfaces of X-ray cassettes to assess contamination with methicillin-resistant Staphylococcus aureus (MRSA). The surfaces of 37 X-ray cassettes stored in a radiology department were cultured using mannitol salt agar containing 6 µg/mL oxacillin. Suspected methicillin-resistant staphylococcal colonies were isolated and identified by biochemical testing. Pulsed-field gel electrophoresis (PFGE) analysis was performed to determine the clonal relationships of the contaminants. Six X-ray cassettes (16.2%) were contaminated with MRSA. During the isolation procedure, we also detected 19 X-ray cassettes (51.4%) contaminated with methicillin-resistant Staphylococcus haemolyticus (MRSH), identified as yellow colonies resembling MRSA on mannitol salt agar. PFGE analysis of the MRSA and MRSH isolates revealed that most isolates of each organism were identical or closely related to each other, suggesting a common source of contamination. X-ray cassettes, which are commonly in direct contact with patients, were contaminated with MRSA and MRSH. In hospital environments, contaminated X-ray cassettes may serve as fomites for methicillin-resistant staphylococci.

  20. A novel flow cytometric HTS assay reveals functional modulators of ATP binding cassette transporter ABCB6.

    Directory of Open Access Journals (Sweden)

    Kishore Polireddy

    Full Text Available ABCB6 is a member of the adenosine triphosphate (ATP-binding cassette family of transporter proteins that is increasingly recognized as a relevant physiological and therapeutic target. Evaluation of modulators of ABCB6 activity would pave the way toward a more complete understanding of the significance of this transport process in tumor cell growth, proliferation and therapy-related drug resistance. In addition, this effort would improve our understanding of the function of ABCB6 in normal physiology with respect to heme biosynthesis, and cellular adaptation to metabolic demand and stress responses. To search for modulators of ABCB6, we developed a novel cell-based approach that, in combination with flow cytometric high-throughput screening (HTS, can be used to identify functional modulators of ABCB6. Accumulation of protoporphyrin, a fluorescent molecule, in wild-type ABCB6 expressing K562 cells, forms the basis of the HTS assay. Screening the Prestwick Chemical Library employing the HTS assay identified four compounds, benzethonium chloride, verteporfin, tomatine hydrochloride and piperlongumine, that reduced ABCB6 mediated cellular porphyrin levels. Validation of the identified compounds employing the hemin-agarose affinity chromatography and mitochondrial transport assays demonstrated that three out of the four compounds were capable of inhibiting ABCB6 mediated hemin transport into isolated mitochondria. However, only verteporfin and tomatine hydrochloride inhibited ABCB6's ability to compete with hemin as an ABCB6 substrate. This assay is therefore sensitive, robust, and suitable for automation in a high-throughput environment as demonstrated by our identification of selective functional modulators of ABCB6. Application of this assay to other libraries of synthetic compounds and natural products is expected to identify novel modulators of ABCB6 activity.

  1. ATP Binding Cassette Transporter Mediates Both Heme and Pesticide Detoxification in Tick Midgut Cells.

    Directory of Open Access Journals (Sweden)

    Flavio Alves Lara

    Full Text Available In ticks, the digestion of blood occurs intracellularly and proteolytic digestion of hemoglobin takes place in a dedicated type of lysosome, the digest vesicle, followed by transfer of the heme moiety of hemoglobin to a specialized organelle that accumulates large heme aggregates, called hemosomes. In the present work, we studied the uptake of fluorescent metalloporphyrins, used as heme analogs, and amitraz, one of the most regularly used acaricides to control cattle tick infestations, by Rhipicephalus (Boophilus microplus midgut cells. Both compounds were taken up by midgut cells in vitro and accumulated inside the hemosomes. Transport of both molecules was sensitive to cyclosporine A (CsA, a well-known inhibitor of ATP binding cassette (ABC transporters. Rhodamine 123, a fluorescent probe that is also a recognized ABC substrate, was similarly directed to the hemosome in a CsA-sensitive manner. Using an antibody against conserved domain of PgP-1-type ABC transporter, we were able to immunolocalize PgP-1 in the digest vesicle membranes. Comparison between two R. microplus strains that were resistant and susceptible to amitraz revealed that the resistant strain detoxified both amitraz and Sn-Pp IX more efficiently than the susceptible strain, a process that was also sensitive to CsA. A transcript containing an ABC transporter signature exhibited 2.5-fold increased expression in the amitraz-resistant strain when compared with the susceptible strain. RNAi-induced down-regulation of this ABC transporter led to the accumulation of metalloporphyrin in the digestive vacuole, interrupting heme traffic to the hemosome. This evidence further confirms that this transcript codes for a heme transporter. This is the first report of heme transport in a blood-feeding organism. While the primary physiological function of the hemosome is to detoxify heme and attenuate its toxicity, we suggest that the use of this acaricide detoxification pathway by ticks may

  2. Improving yeast strains using recyclable integration cassettes, for the production of plant terpenoids

    Directory of Open Access Journals (Sweden)

    Johnson Christopher B

    2011-01-01

    Full Text Available Abstract Background Terpenoids constitute a large family of natural products, attracting commercial interest for a variety of uses as flavours, fragrances, drugs and alternative fuels. Saccharomyces cerevisiae offers a versatile cell factory, as the precursors of terpenoid biosynthesis are naturally synthesized by the sterol biosynthetic pathway. Results S. cerevisiae wild type yeast cells, selected for their capacity to produce high sterol levels were targeted for improvement aiming to increase production. Recyclable integration cassettes were developed which enable the unlimited sequential integration of desirable genetic elements (promoters, genes, termination sequence at any desired locus in the yeast genome. The approach was applied on the yeast sterol biosynthetic pathway genes HMG2, ERG20 and IDI1 resulting in several-fold increase in plant monoterpene and sesquiterpene production. The improved strains were robust and could sustain high terpenoid production levels for an extended period. Simultaneous plasmid-driven co-expression of IDI1 and the HMG2 (K6R variant, in the improved strain background, maximized monoterpene production levels. Expression of two terpene synthase enzymes from the sage species Salvia fruticosa and S. pomifera (SfCinS1, SpP330 in the modified yeast cells identified a range of terpenoids which are also present in the plant essential oils. Co-expression of the putative interacting protein HSP90 with cineole synthase 1 (SfCinS1 also improved production levels, pointing to an additional means to improve production. Conclusions Using the developed molecular tools, new yeast strains were generated with increased capacity to produce plant terpenoids. The approach taken and the durability of the strains allow successive rounds of improvement to maximize yields.

  3. The evolution of the bacterial luciferase gene cassette (lux) as a real-time bioreporter.

    Science.gov (United States)

    Close, Dan; Xu, Tingting; Smartt, Abby; Rogers, Alexandra; Crossley, Robert; Price, Sarah; Ripp, Steven; Sayler, Gary

    2012-01-01

    The bacterial luciferase gene cassette (lux) is unique among bioluminescent bioreporter systems due to its ability to synthesize and/or scavenge all of the substrate compounds required for its production of light. As a result, the lux system has the unique ability to autonomously produce a luminescent signal, either continuously or in response to the presence of a specific trigger, across a wide array of organismal hosts. While originally employed extensively as a bacterial bioreporter system for the detection of specific chemical signals in environmental samples, the use of lux as a bioreporter technology has continuously expanded over the last 30 years to include expression in eukaryotic cells such as Saccharomyces cerevisiae and even human cell lines as well. Under these conditions, the lux system has been developed for use as a biomedical detection tool for toxicity screening and visualization of tumors in small animal models. As the technologies for lux signal detection continue to improve, it is poised to become one of the first fully implantable detection systems for intra-organismal optical detection through direct marriage to an implantable photon-detecting digital chip. This review presents the basic biochemical background that allows the lux system to continuously autobioluminesce and highlights the important milestones in the use of lux-based bioreporters as they have evolved from chemical detection platforms in prokaryotic bacteria to rodent-based tumorigenesis study targets. In addition, the future of lux imaging using integrated circuit microluminometry to image directly within a living host in real-time will be introduced and its role in the development of dose/response therapeutic systems will be highlighted.

  4. A Precisely Regulated Gene Expression Cassette Potently Modulates Metastasis and Survival in Multiple Solid Cancers

    Science.gov (United States)

    Yu, Kun; Ganesan, Kumaresan; Tan, Lay Keng; Laban, Mirtha; Wu, Jeanie; Zhao, Xiao Dong; Li, Hongmin; Leung, Carol Ho Wing; Zhu, Yansong; Wei, Chia Lin; Hooi, Shing Chuan; Miller, Lance; Tan, Patrick

    2008-01-01

    Successful tumor development and progression involves the complex interplay of both pro- and anti-oncogenic signaling pathways. Genetic components balancing these opposing activities are likely to require tight regulation, because even subtle alterations in their expression may disrupt this balance with major consequences for various cancer-associated phenotypes. Here, we describe a cassette of cancer-specific genes exhibiting precise transcriptional control in solid tumors. Mining a database of tumor gene expression profiles from six different tissues, we identified 48 genes exhibiting highly restricted levels of gene expression variation in tumors (n = 270) compared to nonmalignant tissues (n = 71). Comprising genes linked to multiple cancer-related pathways, the restricted expression of this “Poised Gene Cassette” (PGC) was robustly validated across 11 independent cohorts of ∼1,300 samples from multiple cancer types. In three separate experimental models, subtle alterations in PGC expression were consistently associated with significant differences in metastatic and invasive potential. We functionally confirmed this association in siRNA knockdown experiments of five PGC genes (p53CSV, MAP3K11, MTCH2, CPSF6, and SKIP), which either directly enhanced the invasive capacities or inhibited the proliferation of AGS cancer cells. In primary tumors, similar subtle alterations in PGC expression were also repeatedly associated with clinical outcome in multiple cohorts. Taken collectively, these findings support the existence of a common set of precisely controlled genes in solid tumors. Since inducing small activity changes in these genes may prove sufficient to potently influence various tumor phenotypes such as metastasis, targeting such precisely regulated genes may represent a promising avenue for novel anti-cancer therapies. PMID:18636107

  5. ATP Binding Cassette Transporter Mediates Both Heme and Pesticide Detoxification in Tick Midgut Cells

    Science.gov (United States)

    Lara, Flavio Alves; Pohl, Paula C.; Gandara, Ana Caroline; Ferreira, Jessica da Silva; Nascimento-Silva, Maria Clara; Bechara, Gervásio Henrique; Sorgine, Marcos H. F.; Almeida, Igor C.; Vaz, Itabajara da Silva; Oliveira, Pedro L.

    2015-01-01

    In ticks, the digestion of blood occurs intracellularly and proteolytic digestion of hemoglobin takes place in a dedicated type of lysosome, the digest vesicle, followed by transfer of the heme moiety of hemoglobin to a specialized organelle that accumulates large heme aggregates, called hemosomes. In the present work, we studied the uptake of fluorescent metalloporphyrins, used as heme analogs, and amitraz, one of the most regularly used acaricides to control cattle tick infestations, by Rhipicephalus (Boophilus) microplus midgut cells. Both compounds were taken up by midgut cells in vitro and accumulated inside the hemosomes. Transport of both molecules was sensitive to cyclosporine A (CsA), a well-known inhibitor of ATP binding cassette (ABC) transporters. Rhodamine 123, a fluorescent probe that is also a recognized ABC substrate, was similarly directed to the hemosome in a CsA-sensitive manner. Using an antibody against conserved domain of PgP-1-type ABC transporter, we were able to immunolocalize PgP-1 in the digest vesicle membranes. Comparison between two R. microplus strains that were resistant and susceptible to amitraz revealed that the resistant strain detoxified both amitraz and Sn-Pp IX more efficiently than the susceptible strain, a process that was also sensitive to CsA. A transcript containing an ABC transporter signature exhibited 2.5-fold increased expression in the amitraz-resistant strain when compared with the susceptible strain. RNAi-induced down-regulation of this ABC transporter led to the accumulation of metalloporphyrin in the digestive vacuole, interrupting heme traffic to the hemosome. This evidence further confirms that this transcript codes for a heme transporter. This is the first report of heme transport in a blood-feeding organism. While the primary physiological function of the hemosome is to detoxify heme and attenuate its toxicity, we suggest that the use of this acaricide detoxification pathway by ticks may represent a new

  6. The Evolution of the Bacterial Luciferase Gene Cassette (lux as a Real-Time Bioreporter

    Directory of Open Access Journals (Sweden)

    Gary Sayler

    2012-01-01

    Full Text Available The bacterial luciferase gene cassette (lux is unique among bioluminescent bioreporter systems due to its ability to synthesize and/or scavenge all of the substrate compounds required for its production of light. As a result, the lux system has the unique ability to autonomously produce a luminescent signal, either continuously or in response to the presence of a specific trigger, across a wide array of organismal hosts. While originally employed extensively as a bacterial bioreporter system for the detection of specific chemical signals in environmental samples, the use of lux as a bioreporter technology has continuously expanded over the last 30 years to include expression in eukaryotic cells such as Saccharomyces cerevisiae and even human cell lines as well. Under these conditions, the lux system has been developed for use as a biomedical detection tool for toxicity screening and visualization of tumors in small animal models. As the technologies for lux signal detection continue to improve, it is poised to become one of the first fully implantable detection systems for intra-organismal optical detection through direct marriage to an implantable photon-detecting digital chip. This review presents the basic biochemical background that allows the lux system to continuously autobioluminesce and highlights the important milestones in the use of lux-based bioreporters as they have evolved from chemical detection platforms in prokaryotic bacteria to rodent-based tumorigenesis study targets. In addition, the future of lux imaging using integrated circuit microluminometry to image directly within a living host in real-time will be introduced and its role in the development of dose/response therapeutic systems will be highlighted.

  7. Improving yeast strains using recyclable integration cassettes, for the production of plant terpenoids

    Science.gov (United States)

    2011-01-01

    Background Terpenoids constitute a large family of natural products, attracting commercial interest for a variety of uses as flavours, fragrances, drugs and alternative fuels. Saccharomyces cerevisiae offers a versatile cell factory, as the precursors of terpenoid biosynthesis are naturally synthesized by the sterol biosynthetic pathway. Results S. cerevisiae wild type yeast cells, selected for their capacity to produce high sterol levels were targeted for improvement aiming to increase production. Recyclable integration cassettes were developed which enable the unlimited sequential integration of desirable genetic elements (promoters, genes, termination sequence) at any desired locus in the yeast genome. The approach was applied on the yeast sterol biosynthetic pathway genes HMG2, ERG20 and IDI1 resulting in several-fold increase in plant monoterpene and sesquiterpene production. The improved strains were robust and could sustain high terpenoid production levels for an extended period. Simultaneous plasmid-driven co-expression of IDI1 and the HMG2 (K6R) variant, in the improved strain background, maximized monoterpene production levels. Expression of two terpene synthase enzymes from the sage species Salvia fruticosa and S. pomifera (SfCinS1, SpP330) in the modified yeast cells identified a range of terpenoids which are also present in the plant essential oils. Co-expression of the putative interacting protein HSP90 with cineole synthase 1 (SfCinS1) also improved production levels, pointing to an additional means to improve production. Conclusions Using the developed molecular tools, new yeast strains were generated with increased capacity to produce plant terpenoids. The approach taken and the durability of the strains allow successive rounds of improvement to maximize yields. PMID:21276210

  8. ATP-binding cassette (ABC transporters in normal and pathological lung

    Directory of Open Access Journals (Sweden)

    Timmer-Bosscha Hetty

    2005-06-01

    Full Text Available Abstract ATP-binding cassette (ABC transporters are a family of transmembrane proteins that can transport a wide variety of substrates across biological membranes in an energy-dependent manner. Many ABC transporters such as P-glycoprotein (P-gp, multidrug resistance-associated protein 1 (MRP1 and breast cancer resistance protein (BCRP are highly expressed in bronchial epithelium. This review aims to give new insights in the possible functions of ABC molecules in the lung in view of their expression in different cell types. Furthermore, their role in protection against noxious compounds, e.g. air pollutants and cigarette smoke components, will be discussed as well as the (malfunction in normal and pathological lung. Several pulmonary drugs are substrates for ABC transporters and therefore, the delivery of these drugs to the site of action may be highly dependent on the presence and activity of many ABC transporters in several cell types. Three ABC transporters are known to play an important role in lung functioning. Mutations in the cystic fibrosis transmembrane conductance regulator (CFTR gene can cause cystic fibrosis, and mutations in ABCA1 and ABCA3 are responsible for respectively Tangier disease and fatal surfactant deficiency. The role of altered function of ABC transporters in highly prevalent pulmonary diseases such as asthma or chronic obstructive pulmonary disease (COPD have hardly been investigated so far. We especially focused on polymorphisms, knock-out mice models and in vitro results of pulmonary research. Insight in the function of ABC transporters in the lung may open new ways to facilitate treatment of lung diseases.

  9. Unprecedented enhancement of transient gene expression from minimal cassettes using a double terminator.

    Science.gov (United States)

    Beyene, Getu; Buenrostro-Nava, Marco T; Damaj, Mona B; Gao, San-Ji; Molina, Joe; Mirkov, T Erik

    2011-01-01

    The potential of using vector-free minimal gene cassettes (MGCs) with a double terminator for the enhancement and stabilization of transgene expression was tested in sugarcane biolistic transformation. The MGC system used consisted of the enhanced yellow fluorescent protein (EYFP) reporter gene driven by the maize ubiquitin-1 (Ubi) promoter and a single or double terminator from nopaline synthase (Tnos) or/and Cauliflower mosaic virus 35S (35ST). Transient EYFP expression from Tnos or 35ST single terminator MGC was very low and unstable, typically peaking early (8-16 h) and diminishing rapidly (48-72 h) after bombardment. Addition of a ~260 bp vector sequence (VS) to the single MGC downstream of Tnos (Tnos + VS) or 35ST (35ST + VS) enhanced EYFP expression by 1.25- to 25-fold. However, a much more significant increase in EYFP expression was achieved when the VS in 35ST + VS was replaced by Tnos to generate a 35ST-Tnos double terminator MGC, reaching its maximum at 24 h post-bombardment. The enhanced EYFP expression from the double terminator MGC was maintained for a long period of time (168 h), resulting in an overall increase of 5- to 65-fold and 10- to 160-fold as compared to the 35ST and Tnos single terminator MGCs, respectively. The efficiency of the double terminator MGC in enhancing EYFP expression was also demonstrated in sorghum and tobacco, suggesting that the underlying mechanism is highly conserved among monocots and dicots. Our results also suggest the involvement of posttranscriptional gene silencing in the reduced and unstable transgene expression from single terminator MGCs in plants.

  10. Self-Control and the Effects of Movie Alcohol Portrayals on Immediate Alcohol Consumption in Male College Students

    Science.gov (United States)

    Koordeman, Renske; Anschutz, Doeschka J.; Engels, Rutger C. M. E.

    2015-01-01

    Background: In movies, alcohol-related cues are frequently depicted and there is evidence for a link between movie alcohol cues and immediate alcohol consumption. Less is known about factors influencing immediate effects movie alcohol exposure on drinking. The exertion of self-control is thought to be important in avoiding or resisting certain temptations. Aims: The aim of the present study was to assess the immediate effects of movie alcohol portrayals on drinking of male social drinkers and to assess the moderating role of self-control in this relation. It was hypothesized that participants would drink more when exposed to movie alcohol portrayals and that especially participants with low self-control would be affected by these portrayals. Methods: A between-subjects design comparing two movie conditions (alcohol or no portrayal of alcohol) was used, in which 154 pairs of male friends (ages 18–30) watched a 1-h movie in a semi-naturalistic living room setting. Their alcohol consumption while watching was examined. Participants completed a questionnaire assessing self-control as well as their self-reported weekly alcohol use. A multivariate regression analysis was conducted to test the effects of movie condition on alcohol comsumption. Results: Self-control moderated the relation between movie condition and alcohol consumption. Assignment to the alcohol movie condition increased alcohol consumption during the movie for males with high self-control but not for males with low self-control. Conclusion: Viewing a movie with alcohol portrayals can lead to higher alcohol consumption in a specific sample of young men while watching a movie. PMID:25691873

  11. Self-control and the Effects of Movie Alcohol Portrayals on Immediate Alcohol Consumption in Male College Students

    Directory of Open Access Journals (Sweden)

    Renske eKoordeman

    2015-02-01

    Full Text Available AbstractBackground: In movies alcohol-related cues are frequently depicted and there is evidence for a link between movie alcohol cues and immediate alcohol consumption. Less is known about factors influencing immediate effects movie alcohol exposure on drinking. The exertion of self-control is thought be important in avoiding or resisting certain temptations. Aims: The aim of the present study was to assess the immediate effects of movie alcohol portrayals on drinking of male social drinkers and to assess the moderating role of self-control in this relation. It was hypothesized that participants would drink more when exposed to movie alcohol portrayals and that especially participants with low self-control would be affected by these portrayals.Methods: A between-subjects design comparing two movie conditions (alcohol or no portrayal of alcohol was used, in which 154 pairs of male friends (ages 18-30 watched a 1-hour movie in a semi-naturalistic living room setting. Their alcohol consumption while watching was examined. Participants completed a questionnaire assessing self-control as well as their self-reported weekly alcohol use. A multivariate regression analysis was conducted to test the effects of movie condition on alcohol comsumption. Results: Self-control moderated the relation between movie condition and alcohol consumption. Assignment to the alcohol movie condition increased alcohol consumption during the movie for males with high self-control but not for males with low self-control. Conclusion: Viewing a movie with alcohol portrayals can lead to higher alcohol consumption in a specific sample of young men while watching a movie.

  12. Self-control and the effects of movie alcohol portrayals on immediate alcohol consumption in male college students.

    Science.gov (United States)

    Koordeman, Renske; Anschutz, Doeschka J; Engels, Rutger C M E

    2014-01-01

    In movies, alcohol-related cues are frequently depicted and there is evidence for a link between movie alcohol cues and immediate alcohol consumption. Less is known about factors influencing immediate effects movie alcohol exposure on drinking. The exertion of self-control is thought to be important in avoiding or resisting certain temptations. The aim of the present study was to assess the immediate effects of movie alcohol portrayals on drinking of male social drinkers and to assess the moderating role of self-control in this relation. It was hypothesized that participants would drink more when exposed to movie alcohol portrayals and that especially participants with low self-control would be affected by these portrayals. A between-subjects design comparing two movie conditions (alcohol or no portrayal of alcohol) was used, in which 154 pairs of male friends (ages 18-30) watched a 1-h movie in a semi-naturalistic living room setting. Their alcohol consumption while watching was examined. Participants completed a questionnaire assessing self-control as well as their self-reported weekly alcohol use. A multivariate regression analysis was conducted to test the effects of movie condition on alcohol comsumption. Self-control moderated the relation between movie condition and alcohol consumption. Assignment to the alcohol movie condition increased alcohol consumption during the movie for males with high self-control but not for males with low self-control. Viewing a movie with alcohol portrayals can lead to higher alcohol consumption in a specific sample of young men while watching a movie.

  13. Capturing and portraying science student teachers' pedagogical content knowledge through CoRe construction

    Science.gov (United States)

    Thongnoppakun, Warangkana; Yuenyong, Chokchai

    2018-01-01

    Pedagogical content knowledge (PCK) is an essential kind of knowledge that teacher have for teaching particular content to particular students for enhance students' understanding, therefore, teachers with adequate PCK can give content to their students in an understandable way rather than transfer subject matter knowledge to learner. This study explored science student teachers' PCK for teaching science using Content representation base methodology. Research participants were 68 4th year science student teachers from department of General Science, faculty of Education, Phuket Rajabhat University. PCK conceptualization for teaching science by Magnusson et al. (1999) was applied as a theoretical framework in this study. In this study, Content representation (CoRe) by Loughran et al. (2004) was employed as research methodology in the lesson preparation process. In addition, CoRe consisted of eight questions (CoRe prompts) that designed to elicit and portray teacher's PCK for teaching science. Data were collected from science student teachers' CoRes design for teaching a given topic and student grade. Science student teachers asked to create CoRes design for teaching in topic `Motion in one direction' for 7th grade student and further class discussion. Science student teachers mostly created a same group of science concepts according to subunits of school science textbook rather than planned and arranged content to support students' understanding. Furthermore, they described about the effect of student's prior knowledge and learning difficulties such as students' knowledge of Scalar and Vector quantity; and calculating skill. These responses portrayed science student teacher's knowledge of students' understanding of science and their content knowledge. However, they still have inadequate knowledge of instructional strategies and activities for enhance student learning. In summary, CoRes design can represented holistic overviews of science student teachers' PCK related

  14. Exercise portrayal in children’s television programs: analysis of the UK and Irish programming

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    Scully P

    2016-09-01

    Full Text Available Paul Scully,1 Orlaith Reid,1 Alan P Macken,1–3 Mark Healy,4 Jean Saunders,4 Des Leddin,3,5 Walter Cullen,3 Colum P Dunne,3 Clodagh S O’Gorman1–3,5 1The Children’s Ark, University Hospital Limerick, Limerick, 2National Children’s Research Centre, Dublin, 3Centre for Interventions in Infection, Inflammation & Immunity (4i, Graduate Entry Medical School, 4C-Star, University of Limerick, Limerick, Ireland; 5Department of Medicine, Dalhousie University, Halifax, Canada Background: Television watching is obesogenic due to its sedentary nature and programming content, which influences children. Few studies have examined exercise placement within children-specific programming. This study aimed to investigate the frequency and type of exercise placement in children-specific television broadcasts and to compare placements on the UK and Irish television channels.Methods: Content analysis for five weekdays’ worth of children-specific television broadcasting totaling 82.5 hours on both the UK (British Broadcasting Corporation and Irish (Radió Teilifís Éireann television channels was performed. For the purposes of comparing the UK and Irish placements, analysis was restricted to programming broadcast between 6 am and 11.30 am. Exercise placements were coded based on type of activity, activity context, activity motivating factors and outcome, and characters involved.Results: A total of 780 cues were recorded during the total recording period. A wide variety of sports were depicted, but dancing-related cues were most commonly seen (n=163, 23.3%, with the majority of cues being of mild (n=365, 65.9% or moderate (n=172, 31.0% intensity. The majority of cues were associated with a positive outcome (n=404, 61.4%, and social motivations were most commonly seen (n=289, 30.3%. The Irish and the UK portrayals were broadly similar.Conclusion: This study highlights the wide variety of sports portrayed and the active effort undertaken by television

  15. Impulsivity Moderates the Effects of Movie Alcohol Portrayals on Adolescents’ Willingness to Drink

    Science.gov (United States)

    Gibbons, Frederick X.; Kingsbury, John H.; Wills, Thomas A.; Finneran, Stephanie D.; Cin, Sonya Dal; Gerrard, Meg

    2016-01-01

    This study examined impulsivity as a moderator of adolescents’ reactions to positive vs. negative portrayals of drinking in American movie clips. Impulsivity, along with willingness and intentions to drink in the future, were assessed in a pretest session. In the experimental sessions, adolescents viewed a series of clips that showed drinking associated with either positive outcomes (e.g., social facilitation) or negative outcomes (fights, arguments). A third group viewed clips with similar positive or negative outcomes, but no alcohol consumption. All participants then responded to an implicit measure of attentional bias regarding alcohol (a dot probe), followed by explicit alcohol measures (self-reports of willingness and intentions to drink). Hypotheses, based on dual-processing theories, were: a) high-impulsive adolescents would respond more favorably than low-impulsive adolescents to the positive clips, but not the negative clips; and b) this difference in reactions to the positive clips would be larger on the willingness than the intention measures. Results supported the hypotheses: Adolescents high in impulsivity reported the highest willingness to drink in the positive-clip condition, but were slightly less willing than others in the negative-clip condition. In addition, results on the dot probe task indicated that reaction times to alcohol words were negatively correlated with changes in alcohol willingness, but not intention; i.e., the faster their response to the alcohol words, the more their willingness increased. The results highlight the utility of a dual-processing perspective on media influence. PMID:27099959

  16. Pseudomyxoma peritonei – two novel orthotopic mouse models portray the PMCA-I histopathologic subtype

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    Wiig Johan N

    2007-06-01

    Full Text Available Abstract Background Pseudomyxoma peritonei (PMP is a rare malignant disease, most commonly originating from appendiceal lesions and characterized by accumulation of mucinous tumor tissue in the peritoneal cavity. Since the disease is infrequent, the task of carrying out studies of treatment efficacy and disease biology in the clinical setting is challenging, warranting the development of relevant in vitro and in vivo PMP models. Methods Human tumor tissue was implanted in the peritoneal cavity of nude mice to establish two orthotopic models exhibiting noninvasive intraperitoneal growth without metastasis development. Results Xenograft tissues have retained essential properties of the original human tumors, such as macro- and microscopic growth patterns, mucin production as well as expression of carcinoembryonal antigen, cytokeratins 20 and 7 and the proliferation marker pKi67. Upon microscopic examination, the human tumors were categorized as the PMCA-I (peritoneal mucinous carcinomatosis of intermediate features subtype, which was conserved through 14 examined passages in mice, for the first time modeling this particular histopathologic category. Conclusion In conclusion, two novel orthotopic models of human PMP have been established that consistently portray a distinct histopathologic subtype and reflect essential human tumor properties. Xenografts can easily and reproducibly be transferred to new generations of mice with acceptable passage periods, rendering the models as attractive tools for further studies of PMP biology and treatment.

  17. Rural print media portrayal of secondhand smoke and smoke-free policy.

    Science.gov (United States)

    Helme, Donald W; Rayens, Mary Kay; Kercsmar, Sarah E; Adkins, Sarah M; Amundsen, Shelby J; Lee, Erin; Riker, Carol A; Hahn, Ellen J

    2012-11-01

    The purpose of this article is to describe how the print media portrays secondhand smoke and smoke-free policy in rural communities. Baseline print media clips from an ongoing 5-year study of smoke-free policy development in 40 rural communities were analyzed. The authors hypothesized that community population size would be positively associated with media favorability toward smoke-free policy. Conversely, pounds of tobacco produced and adult smoking prevalence would be negatively associated with media favorability. There was a positive correlation between population size and percentage of articles favorable toward smoke-free policy. The authors did not find a correlation between adult smoking or tobacco produced and media favorability toward smoke-free policy, but we did find a positive relationship between tobacco produced and percentage of pro-tobacco articles and a negative relationship between adult smoking prevalence and percentage of articles about health/comfort. Implications for targeting pro-health media in rural communities as well as policy-based initiatives for tobacco control are discussed.

  18. Portrayal of psychiatric genetics in Australian print news media, 1996-2009.

    Science.gov (United States)

    Wilde, Alex; Bonfiglioli, Catriona; Meiser, Bettina; Mitchell, Philip B; Schofield, Peter R

    2011-10-03

    To investigate how Australian print news media portray psychiatric genetics. Content and framing analysis of a structured sample of print news items about psychiatric genetics published in Australian newspapers between 1996 and 2009. Identify dominant discourses about aetiology of mental illness, and perceived clinical outcomes and implications of psychiatric genetics research. We analysed 406 eligible items about the genetics of psychiatric disorders. News coverage of psychiatric genetics has steadily increased since 1996. Items attributing the aetiology of psychiatric disorders to gene-environment interactions (51%) outnumbered items attributing only genetic (30%) or only environmental factors (20%). Of items that referred to heritability of mental illness, frames of genetic determinism (78%) occurred more frequently than probabilistic frames (22%). Of frames related to genetic prophesy, genetic optimism frames (78%) were used more frequently than frames of genetic pessimism (22%). Psychosocial and ethical implications of psychiatric genetics received comparatively relatively little coverage (23%). The analysis identified 22 predictions about psychiatric genetic discoveries and the availability of molecular-based interventions in psychiatry, most of which (20/22, 91%) failed to manifest by the predicted year. Excessive optimism about the power of genetic technology in psychiatric health care, perceived clinical benefits, and largely unfulfilled predictions about availability of these benefits could encourage unrealistic expectations about future molecular-based treatment options for mental health.

  19. The triumph of pharmaceuticals: the portrayal of depression from 1980 to 2005.

    Science.gov (United States)

    Clarke, Juanne; Gawley, Adele

    2009-03-01

    This paper reports on a study of the media portrayal of depression in all of the 98 articles recovered from high circulating magazines directed towards diverse audiences in Canada and the US from 1980 to 2005. Through qualitative analysis of manifest and latent messages the paper compares stories from the 1980s with those from the 1990s and 2000s with respect to how they answer the following questions about depression: What is it? Where is it? Who gets it? What can or should be done about it? It also looks at the use of metaphors, the deployment of celebrities as representatives of depression and the links made with physical illness. The answers to all questions document a trend over time towards a reductionistic and bio-medicalized notion of depression. Depression moved from a problem explained in a variety of ways in the 1980s to a primarily bio-medical phenomenon in mass magazines in the 1990s and 2000s. Theoretical explanations and practical consequences at the individual, therapeutic and societal levels are considered.

  20. Scheduling the Stork: Media Portrayals of Women's and Physicians' Reasons for Elective Cesarean Delivery.

    Science.gov (United States)

    Campo-Engelstein, Lisa; Howland, Lauren E; Parker, Wendy M; Burcher, Paul

    2015-06-01

    Media interest in cesarean delivery has grown in recent years driven both by rising cesarean delivery rates and the decision by the American College of Obstetrics and Gynecology (ACOG) to permit elective cesarean (EC) delivery. A content analysis of United States newspaper and magazine articles from 2000 to 2013 (n = 131 articles) was completed to understand how the news media portrays ECs. The majority of articles (71.8%) emphasized reasons to support women having an EC, while 38.2 percent of the articles exhibited themes of physician support for ECs. Relatively few articles mentioned reasons against ECs either from the women's perspective (11.5%) or the practitioners' (3.8%). The most common themes given for women choosing ECs were convenience/scheduling (48.9%), avoidance of pain or fear of labor (29.8%), and physical harm to women from vaginal birth (17.6%). Doctors' perspectives were less prevalent in the media than women's perspectives, but when mentioned they were almost exclusively in support of ECs for reasons including avoiding malpractice (28.2%), avoiding physical harm to the woman or baby (16.8%), and timing/scheduling (14.5%). Media coverage suggests ECs are widely accepted by both women and doctors, with women choosing an EC mainly for convenience/scheduling and fear. However, 43 percent of doctors surveyed by ACOG said they were not willing to perform the procedure, and surveys report that mothers rarely request an EC. © 2015 Wiley Periodicals, Inc.

  1. Reporting diet-related health issues through newspapers: portrayal of cardiovascular disease and Type 2 diabetes.

    Science.gov (United States)

    Hellyer, Nicole Elizabeth; Haddock-Fraser, Janet

    2011-02-01

    This study identifies (i) the extent to which newsprint media communicate to their readers the lifestyle factors associated with the development of cardiovascular disease and Type 2 diabetes and (ii) newspaper portrayal of social determinants affecting onset of disease. A content analysis of five leading UK national newspapers and their Sunday equivalents was conducted over a 3-month period between January and March 2008. This study shows that cardiovascular disease had much higher press interest than Type 2 diabetes. 'Middle-market' and 'Quality' papers had higher levels of reporting than the 'Popular' press, but the patterns were more complex when the comprehensiveness of reporting was measured within each article. Social determinants affecting disease onset were poorly reported by newspapers, supporting similar research conducted in other countries. This research identifies that there is potential for newspapers to improve their reporting of lifestyle diseases, by including individual and social determinants of disease onset. Lower social classes who read the popular press receive the lowest frequency of reporting and could benefit most from this information. While the research identifies that newspapers are missing the potential to actively communicate and reinforce government health policy, it recognises that the commercial context of the print media may counter such behaviour.

  2. Portraying Urban Functional Zones by Coupling Remote Sensing Imagery and Human Sensing Data

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    Wei Tu

    2018-01-01

    Full Text Available Portraying urban functional zones provides useful insights into understanding complex urban systems and establishing rational urban planning. Although several studies have confirmed the efficacy of remote sensing imagery in urban studies, coupling remote sensing and new human sensing data like mobile phone positioning data to identify urban functional zones has still not been investigated. In this study, a new framework integrating remote sensing imagery and mobile phone positioning data was developed to analyze urban functional zones with landscape and human activity metrics. Landscapes metrics were calculated based on land cover from remote sensing images. Human activities were extracted from massive mobile phone positioning data. By integrating them, urban functional zones (urban center, sub-center, suburbs, urban buffer, transit region and ecological area were identified by a hierarchical clustering. Finally, gradient analysis in three typical transects was conducted to investigate the pattern of landscapes and human activities. Taking Shenzhen, China, as an example, the conducted experiment shows that the pattern of landscapes and human activities in the urban functional zones in Shenzhen does not totally conform to the classical urban theories. It demonstrates that the fusion of remote sensing imagery and human sensing data can characterize the complex urban spatial structure in Shenzhen well. Urban functional zones have the potential to act as bridges between the urban structure, human activity and urban planning policy, providing scientific support for rational urban planning and sustainable urban development policymaking.

  3. Shame on Me? Shame on You! Emotional Reactions to Cinematic Portrayals of the Holocaust

    Directory of Open Access Journals (Sweden)

    Johannes Kopf-Beck

    2017-08-01

    Full Text Available The media are playing an increasingly important role in teaching the public about the history of the Holocaust. In Germany, however, Holocaust documentaries have been criticized for eliciting unintended, adverse reactions among the viewers, such as distancing from the victims or calling for closing the books on the past. This criticism stems from the concern that such reactions pose an obstacle to critical-constructive engagement and coming to terms with history. This study examines the interplay between cinematic representation of the Holocaust, film-induced defensive strategies, and group-based emotions of shame. Based on a content analysis of six different film excerpts, we investigated the mediating effects of four defensiveness strategies (distancing from victims, victim blaming, closeness to perpetrators, and rejection of the relevance of the Holocaust on group-based shame in a sample of 224 pupils from Germany’s third post-war generation in a quasi-experimental field study. The results reveal the complexity of film-portrayals which can foster as well as hinder group-based shame and thus, a constructive dealing with past injustice.

  4. A Videotaped Self-Modelling and Self-Monitoring Treatment Program to Decrease Off-Task Behaviour in Children with Autism

    Science.gov (United States)

    Coyle, Catherine; Cole, Peter

    2004-01-01

    The value of a videotaped self-modelling and self-monitoring treatment program was investigated in the present study. The focus was the effect of the treatment program on the off-task classroom behaviour of 3 male children with autism. The participants were aged between 9 and 11 years. Two of the children were described as severely autistic and…

  5. The efficacy of videotape feedback for enhancing the effects of exposure-based treatment for social anxiety disorder: A controlled investigation

    NARCIS (Netherlands)

    Smits, J.A.J.; Powers, M.B.; Buxkamper, R.; Telch, M.J.

    2006-01-01

    Correcting patients' faulty beliefs concerning social evaluative threats is the hallmark of cognitive-behavioral treatment of social anxiety disorder. The current study examined the efficacy of two videotape feedback procedures as adjuncts to exposure-based treatment. Participants suffering from

  6. Examining the content of weight, nutrition and physical activity advices provided by Dutch practice nurses in primary care: analysis of videotaped consultations

    NARCIS (Netherlands)

    Dillen, S.M. van; Noordman, J.; Dulmen, S. van; Hiddink, G.J.

    2014-01-01

    BACKGROUND/OBJECTIVE: To examine the content of Dutch practice nurses' (PNs') advices about weight, nutrition and physical activity to overweight and obese patients. SUBJECTS/METHODS: A 100 videotaped real-life PN consultations (The Netherlands, 2010/2011) with overweight or obese patients were

  7. Examining the content of weight, nutrition and physical activity advices provided by Dutch practice nurses in primary care: analysis of videotaped consultations.

    NARCIS (Netherlands)

    Dillen, S.M.E. van; Noordman, J.; Dulmen, S. van; Hiddink, G.J.

    2014-01-01

    Background/Objective: To examine the content of Dutch practice nurses’ (PNs’) advices about weight, nutrition and physical activity to overweight and obese patients. Subjects/Methods: A 100 videotaped real-life PN consultations (The Netherlands, 2010/2011) with overweight or obese patients were

  8. Crude Exploration: Portraying Industrial Disaster in Deepwater Horizon, a Film Directed By Peter Berg, 2016 Crude Exploration: Portraying Industrial Disaster in Deepwater Horizon, a Film Directed By Peter Berg, 2016 .

    Science.gov (United States)

    Dixon, Shane; Gawley, Tim

    2017-08-01

    The 2016 film Deepwater Horizon offers a rare portrayal of industrial disaster. It is novel as there are few film-based treatments of this issue. The film enables the public to learn about the disaster, the lives lost, and the stories of survival, but it also provides the opportunity to examine how industrial disaster and, by extension, occupational health and safety may be publicly framed and understood. This article presents an analysis of Deepwater Horizon. Four primary industrial disaster frames are identified in the film: profit maximization, technology and technology failure, managerial conflict, and worker portrayals. Each frame offers advantages and limitations for enhancing public understandings of industrial disaster. Missing from the film is the regulatory environment of the oil drilling industry, whose omission serves to potentially reproduce messages that privilege individualistic, isolated, views of industrial disasters and prioritize immediate over distal causes.

  9. Endothelial ATP-binding cassette G1 in mouse endothelium protects against hemodynamic-induced atherosclerosis

    Energy Technology Data Exchange (ETDEWEB)

    Xue, Shanshan [Department of Physiology and Pathophysiology, Tianjin Medical University, Tianjin, 300070 (China); Department of Pediatrics, Baodi District People’s Hospital of Tianjin City, Tianjin, 301800 (China); Wang, Jiaxing [Department of Physiology and Pathophysiology, Peking University Health Science Center, Beijing, 100191 (China); Zhang, Xu; Shi, Ying; Li, Bochuan; Bao, Qiankun [Department of Physiology and Pathophysiology, Tianjin Medical University, Tianjin, 300070 (China); Pang, Wei [Department of Physiology and Pathophysiology, Peking University Health Science Center, Beijing, 100191 (China); Ai, Ding [Department of Physiology and Pathophysiology, Tianjin Medical University, Tianjin, 300070 (China); Zhu, Yi [Department of Physiology and Pathophysiology, Tianjin Medical University, Tianjin, 300070 (China); Department of Physiology and Pathophysiology, Peking University Health Science Center, Beijing, 100191 (China); He, Jinlong, E-mail: hejinlong@tmu.edu.cn [Department of Physiology and Pathophysiology, Tianjin Medical University, Tianjin, 300070 (China)

    2016-08-19

    Activated vascular endothelium inflammation under persistent hyperlipidemia is the initial step of atherogenesis. ATP-binding cassette G1 (ABCG1) is a crucial factor maintaining sterol and lipid homeostasis by transporting cholesterol efflux to high-density lipoprotein. In this study, we investigated the protective effects of ABCG1 in endothelial inflammation activation during early-stage atherogenesis in mice and the underlying mechanisms. Endothelial cell (EC)-specific ABCG1 transgenic (EC-ABCG1-Tg) mice were generated and cross-bred with low-density lipoprotein receptor–deficient (Ldlr{sup −/−}) mice. After a 4-week Western-type diet, the mice were sacrificed for assessing atherosclerosis. Human umbilical vein ECs were treated with different flows, and ABCG1 was adenovirally overexpressed to investigate the mechanism in vitro. Compared with Ldlr{sup −/−} mouse aortas, EC-ABCG1-Tg/Ldlr{sup −/−} aortas showed decreased early-stage lesions. Furthermore, the lesion area in the EC-ABCG1-Tg/Ldlr{sup −/−} mouse aortic arch but not thoracic aorta was significantly reduced, which suggests a protective role of ABCG1 under atheroprone flow. In vitro, overexpression of ABCG1 attenuated EC activation caused by oscillatory shear stress. Overexpression of ABCG1 blunted cholesterol-activated ECs in vitro. In exploring the mechanisms of ABCG1 attenuating endothelial inflammation, we found that ABCG1 inhibited oscillatory flow-activated nuclear factor kappa B and NLRP3 inflammasome in ECs. ABCG1 may play a protective role in early-stage atherosclerosis by reducing endothelial activation induced by oscillatory shear stress via suppressing the inflammatory response. - Highlights: • EC-ABCG1-Tg mice in a Ldlr{sup −/−} background showed decreased atherosclerosis. • Overexpression of ABCG1 in ECs decreased OSS-induced EC activation. • NLRP3 and NF-κB might be an underlying mechanism of ABCG1 protective role.

  10. Optimized paired-sgRNA/Cas9 cloning and expression cassette triggers high-efficiency multiplex genome editing in kiwifruit.

    Science.gov (United States)

    Wang, Zupeng; Wang, Shuaibin; Li, Dawei; Zhang, Qiong; Li, Li; Zhong, Caihong; Liu, Yifei; Huang, Hongwen

    2018-01-13

    Kiwifruit is an important fruit crop; however, technologies for its functional genomic and molecular improvement are limited. The clustered regulatory interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) system has been successfully applied to genetic improvement in many crops, but its editing capability is variable depending on the different combinations of the synthetic guide RNA (sgRNA) and Cas9 protein expression devices. Optimizing conditions for its use within a particular species is therefore needed to achieve highly efficient genome editing. In this study, we developed a new cloning strategy for generating paired-sgRNA/Cas9 vectors containing four sgRNAs targeting the kiwifruit phytoene desaturase gene (AcPDS). Comparing to the previous method of paired-sgRNA cloning, our strategy only requires the synthesis of two gRNA-containing primers which largely reduces the cost. We further compared efficiencies of paired-sgRNA/Cas9 vectors containing different sgRNA expression devices, including both the polycistronic tRNA-sgRNA cassette (PTG) and the traditional CRISPR expression cassette. We found the mutagenesis frequency of the PTG/Cas9 system was 10-fold higher than that of the CRISPR/Cas9 system, coinciding with the relative expressions of sgRNAs in two different expression cassettes. In particular, we identified large chromosomal fragment deletions induced by the paired-sgRNAs of the PTG/Cas9 system. Finally, as expected, we found both systems can successfully induce the albino phenotype of kiwifruit plantlets regenerated from the G418-resistance callus lines. We conclude that the PTG/Cas9 system is a more powerful system than the traditional CRISPR/Cas9 system for kiwifruit genome editing, which provides valuable clues for optimizing CRISPR/Cas9 editing system in other plants. © 2018 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons

  11. Crystal structure of an integron gene cassette-associated protein from Vibrio cholerae identifies a cationic drug-binding module.

    Directory of Open Access Journals (Sweden)

    Chandrika N Deshpande

    2011-03-01

    Full Text Available The direct isolation of integron gene cassettes from cultivated and environmental microbial sources allows an assessment of the impact of the integron/gene cassette system on the emergence of new phenotypes, such as drug resistance or virulence. A structural approach is being exploited to investigate the modularity and function of novel integron gene cassettes.We report the 1.8 Å crystal structure of Cass2, an integron-associated protein derived from an environmental V. cholerae. The structure defines a monomeric beta-barrel protein with a fold related to the effector-binding portion of AraC/XylS transcription activators. The closest homologs of Cass2 are multi-drug binding proteins, such as BmrR. Consistent with this, a binding pocket made up of hydrophobic residues and a single glutamate side chain is evident in Cass2, occupied in the crystal form by polyethylene glycol. Fluorescence assays demonstrate that Cass2 is capable of binding cationic drug compounds with submicromolar affinity. The Cass2 module possesses a protein interaction surface proximal to its drug-binding cavity with features homologous to those seen in multi-domain transcriptional regulators.Genetic analysis identifies Cass2 to be representative of a larger family of independent effector-binding proteins associated with lateral gene transfer within Vibrio and closely-related species. We propose that the Cass2 family not only has capacity to form functional transcription regulator complexes, but represents possible evolutionary precursors to multi-domain regulators associated with cationic drug compounds.

  12. Novel environmental class 1 integrons and cassette arrays recovered from an on-farm bio-purification plant.

    Science.gov (United States)

    Martini, María Carla; Quiroga, María Paula; Pistorio, Mariano; Lagares, Antonio; Centrón, Daniela; Papa, María Florencia Del

    2017-12-27

    Rapid dissemination and emergence of novel antibiotic resistance genes among bacteria are rising problems worldwide. Since their discovery in clinical isolates in the late 1980s, class 1 integrons have been found in a wide range of bacterial genera and have been extensively studied as contributors to dissemination of antibiotic resistance.The present study aimed to investigate the presence and structure of class 1 integrons in plasmid-carrying bacterial isolates obtained from a biopurification system used for decontamination of pesticide-contaminated water as well as their possible role as reservoir of antimicrobial-resistance gene cassettes. A total of 35 representative isolates were screened for the presence of class 1 integron integrase encoded by intI1. PCR and DNA sequencing revealed the presence of six class 1 integrons with four variable regions: 5'CS-aadA1b-3'CS, 5'CS-aadA2-3'CS, 5'CS-aadA11cΔ-3'CS and 5'CS-dfrB3-aadA1di-catB2-aadA6k-3'CS, the last two being unseen arrays of antimicrobial resistance gene cassettes associated with novel environmental alleles of intI1. These four class 1 integrons were identified as being present in four different genera, including Ochrobactrum, and Variovorax, where class 1 integrons have not been previously reported. The results provide evidence of the biopurification systems as a tank of class 1 integron carrying strains and novel environmental class 1 integron integrases associated with antimicrobial-resistance gene cassette arrays. © FEMS 2017. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  13. Mad tales from Bollywood: the impact of social, political, and economic climate on the portrayal of mental illness in Hindi films.

    Science.gov (United States)

    Bhugra, D

    2005-10-01

    To study the portrayal of mental illness (especially psychosis) in Hindi films since 1950 and to study the influence of prevalent social, political and economic factors on each portrayal. Using two encyclopedias and one source book, films that had mental illness affecting one of the protagonists were identified. The social, economic and political factors were identified using history texts. In the 1960s after India became a Republic, the political climate was one of idealism and as a result the portrayal of mental illness was gentle, more international in its outlook, and used psychoanalytic techniques. In the 1970s and 1980s, as a result of increased political and bureaucratic corruption and an unstable political climate, the portrayals became harder and psychopaths were portrayed more often. In the 1980s, the trend continued with female psychopaths, and avenging women emerged as a major force because the political and judicial systems were seen as impotent in delivering justice. In the 1990s, following economic liberalization, the women were seen and used as possessions in society and the cinema, and portrayals of stalking and morbid jealousy increased. Hindi films since the 1950s appear to have been influenced by changing cultural norms which in turn affected the way mental illness is portrayed.

  14. Structural variations of staphylococcal cassette chromosome mec Type IVa in Staphylococcus aureus clonal complex 8 and unrelated lineages

    DEFF Research Database (Denmark)

    Damborg, Peter Panduro; Bartels, Mette Damkjær; Boye, Kit

    2011-01-01

    PCR mapping of staphylococcal cassette chromosome mec type IVa and adjacent mobile elements in 94 methicillin-resistant Staphylococcus aureus (MRSA) strains identified two primary structures (A and B) that could be further classified into two (A1 and A2) and five (B1 to B5) variants, primarily...... based on structural differences in the orfX-J3 region. While spa type t008 (USA300) invariably contained the A variants, other spa types belonging to clonal complex 8 and unrelated lineages generally contained B variants. These findings have important implications for the typing and identification...

  15. The lysis cassette of bacteriophage ϕKMV encodes a signal-arrest-release endolysin and a pinholin

    OpenAIRE

    Briers, Yves; Peeters, Liesbet M; Volckaert, Guido; Lavigne, Rob

    2011-01-01

    The lysis cassette of Pseudomonas aeruginosa phage ϕKMV encodes a holin, endolysin, Rz and Rz1 in the canonical order. It has a tight organization with a high degree of overlapping genes and is highly conserved (between 96 and 100% identity at the protein level) among several other members of the “phiKMV-like viruses.” The endolysin KMV45 exhibits characteristics as expected for a signal-arrest-release (SAR) endolysin, whereas the holin KMV44 is a typical pinholin. KMV45 is initially secreted...

  16. Structure, function, and evolution of bacterial ATP-binding cassette systems

    Energy Technology Data Exchange (ETDEWEB)

    Davidson, A.L.; Dassa, E.; Orelle, C.; Chen, J. (Purdue)

    2010-07-27

    The ATP-binding cassette (ABC) systems constitute one of the largest superfamilies of paralogous sequences. All ABC systems share a highly conserved ATP-hydrolyzing domain or protein (the ABC; also referred to as a nucleotide-binding domain [NBD]) that is unequivocally characterized by three short sequence motifs (Fig. 1): these are the Walker A and Walker B motifs, indicative of the presence of a nucleotide-binding site, and the signature motif, unique to ABC proteins, located upstream of the Walker B motif (426). Other motifs diagnostic of ABC proteins are also indicated in Fig. 1. The biological significance of these motifs is discussed in Structure, Function, and Dynamics of the ABC. ABC systems are widespread among living organisms and have been detected in all genera of the three kingdoms of life, with remarkable conservation in the primary sequence of the cassette and in the organization of the constitutive domains or subunits (203, 420). ABC systems couple the energy of ATP hydrolysis to an impressively large variety of essential biological phenomena, comprising not only transmembrane (TM) transport, for which they are best known, but also several non-transport-related processes, such as translation elongation (62) and DNA repair (174). Although ABC systems deserve much attention because they are involved in severe human inherited diseases (107), they were first discovered and characterized in detail in prokaryotes, as early as the 1970s (13, 148, 238, 468). The most extensively analyzed systems were the high-affinity histidine and maltose uptake systems of Salmonella enterica serovar Typhimurium and Escherichia coli. Over 2 decades ago, after the completion of the nucleotide sequences encoding these transporters in the respective laboratories of Giovanna Ames and Maurice Hofnung, Hiroshi Nikaido and colleagues noticed that the two systems displayed a global similarity in the nature of their components and, moreover, that the primary sequences of MalK and

  17. [Differentiation of spa types and staphylococcal cassette chromosome mec (SCCmec) in clinical methicillin-resistant Staphylococcus aureus isolated in medical sites of Gdańsk region].

    Science.gov (United States)

    Kasprzyk, Joanna; Piechowicz, Lidia; Wiśniewska, Katarzyna; Dziewit, Łukasz; Bronk, Marek; Świeć, Krystyna

    2015-01-01

    Methicillin-resistant Staphylococcus aureus bacteria are one of the key etiological factors of hospital-acquired and community-acquired infections. MRSA strains have an ability of causing a broad spectrum infections: from a relatively mild skin infections to severe life-threatening systemic infections. They are characterized by multi-drug resistance, virulence of a number of factors, may clonally spread within the hospitals and between hospitals. The study embraced a number of 75 isolates of MRSA isolated from patients of 7 medical sites of the Gdansk region within the period of six months (June to December 2013). Strains have derived from various clinical materials, both of hospitalized patients (n=59) and outpatient (n=16). The isolates were tested for the susceptibility to antimicrobial agents accordance with the guidelines EUCAST. To estimate of the variability of occurrence of S. aureus clones used were standard spa gene, consisting in the amplified polymorphic region of the X gene encoding the protein A gene (spa). After receiving the results, a spa types were identified using international database Ridom Spa Server (www.spaserver.ridom.de). To determine the polymorphism cassette carrying the inecA gene from MRSA strains, used typing five major chromosomal cassette SCCmec (I-V) by multiplex PCR. MRSA population genetic analysis carried out on the basis of typing SCCmec cassettes and spa gene has showed a predominance of strains with SCCmec type II casette (46.7%) and SCCmec IV casette (38.7%). Less frequently detected were strains containing SCCmec I cassette (12.0%) and SCCmec III cassette (2.6%). Spa typing revealed the presence of 13 gene types in MRSA. The most frequently observed spa types were: t151 (24.0%), t003 (16.0%) in strains of the SCCmec II cassette and t437 (16.0%) and t008 (14.8%) in the isolates with SCCmec cassette IV, whereas staphylococcus with the type of spa t011 (12.0%) had SCCmec cassette I. In our population most frequent strains

  18. European Identity as Perceived by Others: the Portrayal of European Political, Cultural and Economic Issues in Ukrainian media

    OpenAIRE

    Ipatenko, Ielyzaveta

    2012-01-01

    The present thesis investigates how European identity is portrayed in Ukrainian media focusing on its political, economic, and cultural dimensions as case studies. Besides the comparative aspect, the thesis also pays attention to temporal changes in the 2002-2012 period. Theoretically, the thesis is based on the social identity theory developed by Tajfel (1981) and Turner (1979). Methodologically, qualitative content analysis is employed in the evaluation of 36 articles from web archives of t...

  19. The Fountain of Stem Cell-Based Youth? Online Portrayals of Anti-Aging Stem Cell Technologies.

    Science.gov (United States)

    Rachul, Christen M; Percec, Ivona; Caulfield, Timothy

    2015-08-01

    The hype surrounding stem cell science has created a market opportunity for the cosmetic industry. Cosmetic and anti-aging products and treatments that make claims regarding stem cell technology are increasingly popular, despite a lack of evidence for safety and efficacy of such products. This study explores how stem cell-based products and services are portrayed to the public through online sources, in order to gain insight into the key messages available to consumers. A content analysis of 100 web pages was conducted to examine the portrayals of stem cell-based cosmetic and anti-aging products and treatments. A qualitative discourse analysis of one web page further examined how language contributes to the portrayals of these products and treatments to public audiences. The majority of web pages portrayed stem cell-based products as ready for public use. Very few web pages substantiated claims with scientific evidence, and even fewer mentioned any risks or limitations associated with stem cell science. The discourse analysis revealed that the framing and use of metaphor obscures the certainty of the efficacy of and length of time for stem cell-based anti-aging technology to be publicly available. This study highlights the need to educate patients and the public on the current limits of stem cell applications in this context. In addition, generating scientific evidence for stem cell-based anti-aging and aesthetic applications is needed for optimizing benefits and minimizing adverse effects for the public. Having more evidence on efficacy and risks will help to protect patients who are eagerly seeking out these treatments. © 2015 The American Society for Aesthetic Plastic Surgery, Inc. Reprints and permission: journals.permissions@oup.com.

  20. Self-Control and the Effects of Movie Alcohol Portrayals on Immediate Alcohol Consumption in Male College Students

    OpenAIRE

    Renske eKoordeman; Doeschka J. eAnschutz; Rutger C. M. E. eEngels

    2015-01-01

    AbstractBackground: In movies alcohol-related cues are frequently depicted and there is evidence for a link between movie alcohol cues and immediate alcohol consumption. Less is known about factors influencing immediate effects movie alcohol exposure on drinking. The exertion of self-control is thought be important in avoiding or resisting certain temptations. Aims: The aim of the present study was to assess the immediate effects of movie alcohol portrayals on drinking of male social drinkers...

  1. How Health Risks Are Pinpointed (or Not) on Social Media: The Portrayal of Waterpipe Smoking on Pinterest.

    Science.gov (United States)

    Guidry, Jeanine; Jin, Yan; Haddad, Linda; Zhang, Yuan; Smith, Joshua

    2016-01-01

    Waterpipe tobacco is among the most rapidly growing trends in tobacco smoking, the growing use and acceptance of which are taking place at a time when cigarette smoking is regarded in an increasingly negative manner (Martinasek, McDermott, & Martini, 2011). Given the health risks and the misperceptions associated with waterpipe smoking, this study focuses on how waterpipe smoking is portrayed and represented on the social media platform Pinterest. In total, 800 Pinterest pins were content analyzed. The vast majority of the sampled pins were primarily image based. More than half of the pins linked to a website external to Pinterest that often redirected the pinner to a commercial website. Waterpipe-focused pins portrayed waterpipe smoking more in a positive light than in a negative light, and conveyed a sense of pleasure, aesthetic, and relaxation, which tended to trigger more repins, likes, and higher levels of engagement. Overall, the risks of waterpipe smoking were not represented on Pinterest, indicating that the social media portrayal of waterpipe smoking needs improvement and could benefit from a public health perspective.

  2. Portrayal of caesarean section in Brazilian women's magazines: 20 year review.

    Science.gov (United States)

    Torloni, Maria Regina; Daher, Silvia; Betrán, Ana Pilar; Widmer, Mariana; Montilla, Pilar; Souza, Joao Paulo; Merialdi, Mario

    2011-01-25

    To assess the quality and comprehensiveness of the information on caesarean section provided in Brazilian women's magazines. Review of articles published during 1988-2008 in top selling women's magazines. Brazil, one of the countries with the highest caesarean section rates in the world. Women's magazines with the largest distribution during the study period, identified through the official national media indexing organisations. Articles with objective scientific information or advice, comments, opinions, or the experience of ordinary women or celebrities on delivery by caesarean section. Sources of information mentioned by the author of the article, the accuracy and completeness of data presented on caesarean section, and alleged reasons why women would prefer to deliver though caesarean section. 118 articles were included. The main cited sources of information were health professionals (78% (n=92) of the articles). 71% (n=84) of the articles reported at least one benefit of caesarean section, and 82% (n=97) reported at least one short term maternal risk of caesarean section. The benefits most often attributed to delivery by caesarean section were reduction of pain and convenience for family or health professionals. The most frequently reported short term maternal risks of caesarean section were increased time to recover and that it is a less natural way of giving birth. Only one third of the articles mentioned any long term maternal risks or perinatal complications associated with caesarean section. Fear of pain was the main reported reason why women would prefer to deliver by caesarean section. Most of the articles published in Brazilian women's magazines do not use optimal sources of information. The portrayal of caesarean section is mostly balanced, not explicitly in favour of one or another route of delivery, but incomplete and may be leading women to underestimate the maternal/perinatal risks associated with this route of delivery.

  3. Portrayal of genetic risk for breast cancer in ethnic and non-ethnic newspapers.

    Science.gov (United States)

    Donelle, L; Hoffman-Goetz, L; Clarke, J N

    2004-01-01

    There has been enormous attention paid to the genetics of breast cancer in this era of genomic medicine. A great deal of the interest has been generated through discourse in the public mass media. However, genetic risk is a probabilistic concept and one that requires adequate numeracy skills. The purpose of this qualitative content analysis was to describe and evaluate the portrayal of genetic risk for breast cancer in mass print media. Mass print newspapers targeting high (Ashkenazi Jews) and low (general Canadian population) genetic risk audiences and published at least monthly, available in English and accessible through public archives at the National Library of Canada, were identified and hand searched for articles on breast cancer. Approximately 47% of breast cancer articles in 6 Jewish newspapers and published between 1996-2000 identified genetics in the title, first or last paragraph compared with 17% of 145 articles in 6 provincial newspapers published in 2000. The description of breast cancer risk was equally problematic in print media targeting high and low risk audiences. Statistics were presented in complex and contradictory ways, with, for example, the confounding of individual and population based risk estimates. Inconsistent messages about the value of genetic screening for breast cancer characterized articles in both ethnic and non-ethnic newspapers. Deciphering the information into a comprehensible form is likely challenging, particularly in light of widespread numeric-literacy limitations. The publication of discrepant research findings and the perplexing statistical information consequently brought into question the credibility of the scientific process and the recommendations of health care professionals.

  4. Portrayal of caesarean section in Brazilian women’s magazines: 20 year review

    Science.gov (United States)

    Daher, Silvia; Betrán, Ana Pilar; Widmer, Mariana; Montilla, Pilar; Souza, Joao Paulo; Merialdi, Mario

    2011-01-01

    Objective To assess the quality and comprehensiveness of the information on caesarean section provided in Brazilian women’s magazines. Design Review of articles published during 1988-2008 in top selling women’s magazines. Setting Brazil, one of the countries with the highest caesarean section rates in the world. Data sources Women’s magazines with the largest distribution during the study period, identified through the official national media indexing organisations. Selection criteria Articles with objective scientific information or advice, comments, opinions, or the experience of ordinary women or celebrities on delivery by caesarean section. Main outcome measures Sources of information mentioned by the author of the article, the accuracy and completeness of data presented on caesarean section, and alleged reasons why women would prefer to deliver though caesarean section. Results 118 articles were included. The main cited sources of information were health professionals (78% (n=92) of the articles). 71% (n=84) of the articles reported at least one benefit of caesarean section, and 82% (n=97) reported at least one short term maternal risk of caesarean section. The benefits most often attributed to delivery by caesarean section were reduction of pain and convenience for family or health professionals. The most frequently reported short term maternal risks of caesarean section were increased time to recover and that it is a less natural way of giving birth. Only one third of the articles mentioned any long term maternal risks or perinatal complications associated with caesarean section. Fear of pain was the main reported reason why women would prefer to deliver by caesarean section. Conclusions Most of the articles published in Brazilian women’s magazines do not use optimal sources of information. The portrayal of caesarean section is mostly balanced, not explicitly in favour of one or another route of delivery, but incomplete and may be leading women to

  5. Impulsivity moderates the effects of movie alcohol portrayals on adolescents' willingness to drink.

    Science.gov (United States)

    Gibbons, Frederick X; Kingsbury, John H; Wills, Thomas A; Finneran, Stephanie D; Dal Cin, Sonya; Gerrard, Meg

    2016-05-01

    This study examined impulsivity as a moderator of adolescents' reactions to positive versus negative portrayals of drinking in American movie clips. Impulsivity, along with willingness and intentions to drink in the future, were assessed in a pretest session. In the experimental sessions, adolescents viewed a series of clips that showed drinking associated with either positive outcomes (e.g., social facilitation) or negative outcomes (fights, arguments). A third group viewed clips with similar positive or negative outcomes, but no alcohol consumption. All participants then responded to an implicit measure of attentional bias regarding alcohol (a dot probe), followed by explicit alcohol measures (self-reports of willingness and intentions to drink). Hypotheses, based on dual-processing theories, were: (a) high-impulsive adolescents would respond more favorably than low-impulsive adolescents to the positive clips, but not the negative clips; and (b) this difference in reactions to the positive clips would be larger on the willingness than the intention measures. Results supported the hypotheses: Adolescents high in impulsivity reported the highest willingness to drink in the positive-clip condition, but were slightly less willing than others in the negative-clip condition. In addition, results on the dot probe task indicated that RTs to alcohol words were negatively correlated with changes in alcohol willingness, but not intention; that is, the faster their response to the alcohol words, the more their willingness increased. The results highlight the utility of a dual-processing perspective on media influence. (PsycINFO Database Record (c) 2016 APA, all rights reserved).

  6. Finding Feminist Literary Reading: Portrayals Of Women In The 1920s Indonesian Literary Writings

    Directory of Open Access Journals (Sweden)

    Diah Ariani Arimbi

    2014-12-01

    Full Text Available Abstract: Modern Indonesian literature can be said to be born around 1920s with the publication of modern Indonesian literary works by Balai Pustaka. Amongst the works published by Balai Pustaka in the 1920s ; there are most popular works namely Sitti Nurbaya (1922 ; Azab dan Sengsara(1927 and Salah Asuhan (1928 representing the tone of 1920s literary productions. This paper aims to look at images of women in those three works written by male authors ; using feminist literary criticism. By means of close reading technique; the study uses feminist literary criticism to examine and (reexamine the images of women portrayed in those three works. The finding shows that on one hand some women are still trapped with the shackle of patriarchy, but, on the other hand, some women are not simply passive victims of patriarchy: these women still attempt to escape from the patriarchal chain and cut out the patriarchal oppression. Key Words: modern Indonesian literature; 1920s; Balai Pustaka; women; feminist literary criticism Abstrak: Sastra Indonesia modern dapat dikatakan lahir sekitar tahun1920-an dengan publikasi karya sastra Indonesia modern oleh Balai Pustaka. Di antara karya yang diterbitkan oleh Balai Pustaka pada tahun 1920-an; terdapat karya yang paling populer seperti Sitti Nurbaya (1922; Azab dan Sengsara (1927; dan Salah Asuhan (1928 yang mewakili suara produksi sastra tahun 1920-an. Makalah ini bertujuan untuk melihat potret perempuan dalam tiga karya yang ditulis oleh penulis laki-laki dengan menggunakan pendekatan kritik sastra feminis. Melalui teknik pembacaan yang mendalam (close reading technique; penelitian ini menggunakan kritik sastra feminis untuk menelaah potret perempuan dalam tiga karya tersebut. Temuan dalam tulisan ini menunjukkan bahwa di satu sisi perempuan masih terbelenggu oleh patriarkat; tetapi di sisi lain perempuan bukanlah korban patriarkat yang pasif: perempuan tetap berupaya untuk keluar dari belenggu ini dan memutus

  7. Portrayal of complementary and alternative medicine for cancer by top online news sites.

    Science.gov (United States)

    Price, Alexandra; Grann, Victor R

    2012-05-01

    Medical research news provided through the World Wide Web is easily accessible to the general public. Thus, it is necessary to understand how research findings released from online news sources are portrayed. The sample includes articles (n=205) published between January 1, 2010 and June 18, 2010 in top online news sites with competitive traffic rankings in the United States as determined by Alexa.com. Google Reader was used to find health-related news articles corresponding to the relevant news sources. Data analysis was performed using SPSS with two-tailed significance values of the χ(2) statistic. A content analysis (n=205 stories) revealed that the majority of complementary and alternative medicine (CAM) modalities for cancer discussed in top online news sources are classified as nutritional therapeutics, and the cancer topic that appeared most frequently was that of prevention. General oncology was the most frequently cited cancer type that was discussed with regard to CAM. Medical journals were the most frequently cited source in CAM/cancer news articles. The majority of news stories on CAM/cancer were neutral in tone, and the relationship between tone and evidence type reported was statistically significant. Observational studies rather than randomized controlled trials were the predominant form of evidence provided for research findings. Overall, the quality of how online news sources report research findings on complementary and alternative medicine therapies for cancer is fairly high. However, certain top online media sources are more reliable and informative than others when it comes to reporting about CAM remedies for cancer.

  8. The portrayal of Africa and Africans in the book of Jeremiah

    Directory of Open Access Journals (Sweden)

    David T. Adamo

    2018-01-01

    Full Text Available Since the 1980s, many Jeremianic scholars have spent much time on the study of the various contentious issues in order to resolve them. However, there has been no unanimous agreement yet. One of these contentious issues is the relationship of the prophet Jeremiah to ancient Africa and Africans which is the main focus of this article. The author of the book Jeremiah made references to Ancient Africa and Africans about 53 times in the Septuagint, and 67 times in the Masoretic Text. This indicates that the prophet Jeremiah is very familiar with ancient Africa and Africans. Using a historical–biographical and theological method of reading Jeremiah, this article examines the portrayal of ancient Africa and Africans in the book of Jeremiah. It is also part of an investigation of the African presence in the Old Testament which, to Africans, is an important moral and self–lifting scholarly exercise. It is also gratifying information in itself to know that Africa and Africans have participated in the drama of redemption which has not been recognised as such by either Eurocentric scholars or by the majority of Africentric scholars themselves. While in the Pentateuch references to Africa and Africans appear more than 577 times, in the Major Prophets there are about 180 references. What this means is that not only the author of the book of Jeremiah, but biblical authors in general are very familiar with ancient Africa and Africans, and deliberately took time to identify them. The continued recognition by scholars and non–scholars of Africa and African presence in the Bible has great implications for Christianity in Africa.

  9. Portrayal of smoking in Nigerian online videos: a medium for tobacco advertising and promotion?

    Directory of Open Access Journals (Sweden)

    Adegoke Oloruntoba Adelufosi

    2014-09-01

    Full Text Available The Nigerian home video industry, popularly known as Nollywood is a booming industry, with increasing numbers of easily accessible online videos. The aim of this study was to analyse the contents of popular Nigerian online videos to determine the prevalence of smoking imageries and their public health implications. Using specific search terms, popular English language and indigenous Yoruba language, Nigerian home videos uploaded on YouTube in 2013 were identified and sorted based on their view counts. Data on smoking related scenes such as smoking incidents, context of tobacco use, depiction of cigarette brand, gender of smokers and film rating were collected. Of the 60 online videos whose contents were assessed in this study, 26 (43.3% had scenes with cigarrete smoking imageries. The mean (SD smoking incident was 2.7 (1.6, giving an average of one smoking incident for every 26 to 27 min of film. More than half (53.8% of the films with tobacco use had high smoking imageries. An average of 2 characters per film smoked, mostly in association with acts of criminality or prostitution (57.7% and alcohol use (57.7%. There were scenes of the main protagonists smoking in 73.1% of the films with scenes of female protagonists smoking (78.9% more than the male protagonists (21.1%. Smoking imageries are common in popular Nigerian online movies. Given the wide reach of online videos, their potential to be viewed by people from different cultures and to negatively influence youngsters, it is important that smoking portrayals in online movies are controlled.

  10. Out of the dissecting room: news media portrayal of human anatomy teaching and research.

    Science.gov (United States)

    Regan de Bere, Sam; Petersen, Alan

    2006-07-01

    Radical changes in medical research and education have recently led to a number of innovative developments in terms of how human anatomy is represented and understood. New ways of introducing medical students to anatomy (including living anatomies and virtual simulations) have provoked widespread debate, with discussion of their relative merits compared to more traditional approaches that use cadaveric dissection. Outside the field of medicine, in the wider public sphere, the practice of anatomical study may often seem mysterious. The dissemination of news on anatomy, we contend, is central to the question of how medical researchers and educators engage with the public. Our analysis of news media coverage in the UK demonstrates that news-making, by giving prominence to certain facts, themes and images, serves to mask issues about anatomy and its practices that need debate. We examine the ways in which news media, through processes of selection and the 'framing' of issues, may perform an agenda-setting role. We draw attention to the use of positive 'awe and amazement' frames including 'miracles of modern science', 'medical heroes', and 'gifts of life', alongside more negative 'guts and gore' coverage including 'Frankenstein', 'Brave New World' and 'Rape of the Body' frames that concentrate on high profile scandals associated with the use and misuse of human bodies, tissues and parts. We also highlight the selective use of commentaries from members of the medical profession, which are more prevalent in positive 'awe and amazement' stories than in stories with negative coverage. We conclude by arguing for greater collaboration between journalists on the one hand, and medical educators and researchers on the other, in the making of news in order to provide portrayals of anatomy which bear a closer relationship to the everyday reality of professional work.

  11. “GLOSSY” POLITICIANS: PORTRAYING WOMEN POLITICIANS IN ROMANIAN CONSUMER MAGAZINES

    Directory of Open Access Journals (Sweden)

    ROMINA SURUGIU

    2012-05-01

    Full Text Available Women consumer magazines (glossies represent the most important part of the specialized media all over the world. The main ingredients of their editorial “recipe” are the positive tone of the articles, and the optimistic, yet shallow approach to all the theme/subjects covered. Magazines are considered to be beautiful objects that inspire people to cherish them. Women magazines have been criticized in feminist media studies for portraying women in a stereotyped way and for encouraging a consumerist behavior among them. The role models offered by these media are mainly taken from the show business and fashion industry. Women politician are rarely present in the pages of these publications, especially in countries as Romania where the political participation of women is one of the lowest in Europe. The paper presents in the first part official figures regarding the political participation of Romanian women, and it discusses the results of the most important academic studies on women and media. A previous research showed, for example, that in a four years period, three important Romanian magazines published only 9 article presenting women politicians. The general assumption in magazines desks (and in the society is that politics is a dirty business that does not match the beautiful world of magazines. The second part will focus on a case study, considered to be relevant for explaining the general image of women politicians and politics in Romanian consumer magazines. A visual analysis (from the popular culture perspective will be done to Elena Udrea’s pictorial feature for Tabu (Taboo magazine (November 2011. The choice of the case study was motivated by the following reasons: Elena Udrea is a controversial, yet successful politician, she has impersonated popular culture icons (Madonna, Jackie, Cleopatra and the feature has generated many positive and negative comments in media.

  12. Concept design of the DEMO divertor cassette-to-vacuum vessel locking system adopting a systems engineering approach

    Energy Technology Data Exchange (ETDEWEB)

    Di Gironimo, G., E-mail: giuseppe.digironimo@unina.it [Università degli Studi di Napoli “Federico II”, Dipartimento di Ingegneria Industriale, Piazzale Tecchio 80, 80135 Napoli (Italy); Carfora, D. [Tampere University of Technology, Korkeakoulunkatu 6, 33720 Tampere (Finland); VTT Technical Research Centre of Finland, Tekniikankatu 1, PO Box 1300, FI-33101 Tampere (Finland); Università degli Studi di Napoli “Federico II”, Dipartimento di Ingegneria Industriale, Piazzale Tecchio 80, 80135 Napoli (Italy); Esposito, G.; Lanzotti, A.; Marzullo, D. [Università degli Studi di Napoli “Federico II”, Dipartimento di Ingegneria Industriale, Piazzale Tecchio 80, 80135 Napoli (Italy); Siuko, M. [VTT Technical Research Centre of Finland, Tekniikankatu 1, PO Box 1300, FI-33101 Tampere (Finland)

    2015-05-15

    Highlights: • An iterative and incremental design process for cassette-to-VV locking system of DEMO divertor is presented. • Three different concepts have been developed with a systematic design approach. • The final concept has been selected with Fuzzy-Analytic Hierarchy Process in virtual reality. - Abstract: This paper deals with pre-concept studies of DEMO divertor cassette-to-vacuum vessel locking system under the work program WP13-DAS-07-T06: Divertor Remote Maintenance System pre-concept study. An iterative design process, consistent with Systems Engineering guidelines and named Iterative and Participative Axiomatic Design Process (IPADeP), is used in this paper to propose new innovative solutions for divertor locking system, which can overcome the difficulties in applying the ITER principles to DEMO. The solutions conceived have been analysed from the structural point of view using the software Ansys and, eventually, evaluated using the methodology known as Fuzzy-Analytic Hierarchy Process. Due to the lack and the uncertainty of the requirements in this early conceptual design stage, the aim is to cover a first iteration of an iterative and incremental process to propose an innovative design concept to be developed in more details as the information will be completed.

  13. A homing endonuclease and the 50-nt ribosomal bypass sequence of phage T4 constitute a mobile DNA cassette

    Science.gov (United States)

    Bonocora, Richard P.; Zeng, Qinglu; Abel, Ethan V.; Shub, David A.

    2011-01-01

    Since its initial description more than two decades ago, the ribosome bypass (or “hop”) sequence of phage T4 stands out as a uniquely extreme example of programmed translational frameshifting. The gene for a DNA topoisomerase subunit of T4 has been split by a 1-kb insertion into two genes that retain topoisomerase function. A second 50-nt insertion, beginning with an in-phase stop codon, is inserted near the start of the newly created downstream gene 60. Instead of terminating at this stop codon, approximately half of the ribosomes skip 50 nucleotides and continue translation in a new reading frame. However, no functions, regulatory or otherwise, have been imputed for the truncated peptide that results from termination at codon 46 or for the bypass sequence itself. Moreover, how this unusual mRNA organization arose and why it is maintained have never been explained. We show here that a homing endonuclease (MobA) is encoded in the insertion that created gene 60, and the mobA gene together with the bypass sequence constitute a mobile DNA cassette. The bypass sequence provides protection against self-cleavage by the nuclease, whereas the nuclease promotes horizontal spread of the entire cassette to related bacteriophages. Group I introns frequently provide protection against self-cleavage by associated homing endonucleases. We present a scenario by which the bypass sequence, which is otherwise a unique genetic element, might have been derived from a degenerate group I intron. PMID:21930924

  14. Emergence of a multidrug-resistant Citrobacter freundii ST8 harboring an unusual VIM-4 gene cassette in Poland.

    Science.gov (United States)

    Majewski, Piotr; Wieczorek, Piotr; Łapuć, Izabela; Ojdana, Dominika; Sieńko, Anna; Sacha, Paweł; Kłoczko, Janusz; Tryniszewska, Elżbieta

    2017-08-01

    The growing incidence of multidrug-resistant (MDR) bacteria is an emerging challenge in modern medicine. The utility of carbapenems, which are considered 'last-line' agents, is being diminished by the growing incidence of various resistance mechanisms in Gram-negative bacteria. A molecular investigation was performed of an MDR carbapenem-resistant Citrobacter freundii of sequence type 8 (ST8) isolated from a hematology patient with acute myeloid leukemia. Multilocus sequence typing and analysis of the nucleotide sequence of the class I integron were performed using PCR and Sanger sequencing. Transformation of the resistance plasmid isolated following the alkaline lysis method was performed using chemically competent E. coli TOP10. Molecular analysis of the carbapenem-resistant C. freundii revealed the presence of the VIM-4 isoenzyme located on the ∼55-kb transferable resistance plasmid. Interestingly, the blaVIM-4 gene was inserted into an unusual gene cassette containing a 169-bp direct repeat of the 3' segment of the blaVIM-4 gene. All unusual gene cassettes containing VIM-DR (direct repeat) described thus far have been harbored by non-fermenters, i.e., Acinetobacter and Pseudomonas, underscoring the importance of resistance determinant mobility, which may go even beyond genus, family, and order boundaries. Great efforts need to be taken to explore pathways of resistance to 'last-resort' antimicrobials, especially among clinically relevant pathogens. Copyright © 2017 The Author(s). Published by Elsevier Ltd.. All rights reserved.

  15. The thnR gene is a negative transcription regulator of the thurincin H genetic cassette in Bacillus thuringiensis subsp. morrisoni.

    Science.gov (United States)

    Casados-Vázquez, Luz E; Bideshi, Dennis K; Barboza-Corona, José E

    2017-03-01

    Thurincin H is a bacteriocin synthesized by some strains of Bacillus thuringiensis. In this study, the thurincin H genetic cassette, which contains ten genes, from a Mexican strain of B. thuringiensis subsp. morrisoni (Btm) was cloned and sequenced. To study the function of the thnR gene component in the cassette, we generated various constructs with or without thnR for expression in Btm. All transformants harboring thnR in recombinant plasmids showed a decrease of ~15 to ~90 % in inhibitory activity against the target strain, Bacillus cereus 183. Importantly, a ~90 % reduction in inhibition occurred with Btm harboring a construct containing thnR alone, suggesting that ThnR, indeed, functions as a negative transcription regulator of the thurincin H cassette. Based on sequence homology, ThnR was classified as a member of the YtrA subfamily of the GntR superfamily of transcriptional regulators.

  16. A vector system for efficient and economical switching of a ura4(+) module to three commonly used antibiotic marker cassettes in Schizosaccharomyces pombe.

    Science.gov (United States)

    Chen, Yinghui; Chen, Lihua; An, Ke; Wang, Yamei; Jin, Quanwen

    2015-11-01

    We describe here the development of a set of plasmid vectors that allow simple, efficient and economical switching of a ura4(+) module in existing Schizosaccharomyces pombe strains to any of the three routinely used antibiotic marker cassettes, kanMX6, hphMX6 and natMX6. In principle, the applications of this system can also be extended to switching ura4(+) for additional MX6 module-based cassettes, such as bleMX6, as long as the antibiotic marker has been cloned into an ura4(+) module-switching vector. We illustrate the application of this set of vectors in exchange of the ura4(+) marker in existing strains with three antibiotic marker cassettes with high efficiency. Copyright © 2015 John Wiley & Sons, Ltd.

  17. Map images portraying flight paths of low-altitude transects over the Arctic Network of national park units and Selawik National Wildlife Refuge, Alaska, July 2013

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — Maps portraying the flight paths for low altitude transects conducted from small aircraft over the National Park Service’s Arctic Network (Bering Land Bridge...

  18. Design and Construction of ctxB-gfp-stxB Gene Cassette and Investigation of Its Expression in E. coli Bl21 (DE3

    Directory of Open Access Journals (Sweden)

    Aghil Esmaeili

    2013-06-01

    Full Text Available Background & Objective: In order to enhance the expression of soluble proteins and facilitate their purification and development of multi-functional polypeptide , chimerical recombinant proteins have been invented . The purpose of this study was to construct ctxB-gfp-stxB gene cassette to measure the uptake and excretion of chimerical antigen in future studies.   Materials & Methods: After preparation of primers for gfp gene as a reporter gene , ctxB and stxB, attempts were made to amplify the genes via the PCR techniques . The amplified genes were clone d in the pGEM vector; and after confirmation of the gene fragments, they were fused as ctxB-gfp-stxB. The gene cassette was thereafter sub-cloned in the pET28a(+ expression vector. E. coli Bl21 (DE3 was transformed by the recombinant vector pET28a(+, and the expression of the recombinant protein was investigated by IPTG induction and SDS-PAGEelectrophoresis.   Results: The amplified genes were cloned in the pGEM vector, and were confirmed via PCR, restriction enzymes, and sequence analyzing system. The confirmed gene fragments were mixed together as ctxB-gfp-stxB . The existence of the gene cassette was confirmed after sub-cloning. The expression was not observed for this gene cassette in E . coli.   Conclusion: The presence of a large number of E. coli rare codons in ctxB and stxB gene sequences precluded the expression of the gene cassette in E. coli; it, therefore, requires the discovery of a suitable host cell for its expression and optimization. Given the gene cassette structure and position of restriction enzymes on the constructed fragment, this gene can be replaced with different genes and can produce a variety of gene fragments.

  19. National newspaper portrayal of U.S. nursing homes: periodic treatment of topic and tone.

    Science.gov (United States)

    Miller, Edward Alan; Tyler, Denise A; Rozanova, Julia; Mor, Vincent

    2012-12-01

    Although observers have long highlighted the relationship of public distrust, government regulation, and media depictions of nursing-home scandals, no study has systematically analyzed the way in which nursing homes have been portrayed in the national media. This study examines how nursing homes were depicted in four leading national newspapers-the New York Times, Washington Post, Chicago Tribune, and Los Angeles Times-from 1999 to 2008. We used keyword searches of the LexisNexis database to identify 1,704 articles pertaining to nursing homes. We then analyzed the content of each article and assessed its tone, themes, prominence, and central actor. We used basic frequencies and descriptive statistics to examine the articles' content, both cross-sectionally and over time. Approximately one-third of the articles were published in 1999/2000, and a comparatively high percentage (12.4%) appeared in 2005. Most were news stories (89.8%), and about one-quarter were on the front page of the newspaper or section. Most focused on government (42.3%) or industry (39.2%) interests, with very few on residents/family (13.3%) and community (5.3%) concerns. Most were negative (45.1%) or neutral (37.0%) in tone, and very few were positive (9.6%) or mixed (8.3%). Common themes were quality (57.0%), financing (33.4%), and negligence/fraud (28.1%). Both tone and themes varied across newspapers and years. Overall, our findings highlight the longitudinal variation in the four widely read newspapers' framing of nursing-home coverage, regarding not only tone but also shifts in media attention from one aspect of this complex policy area to another. The predominantly negative media reports contribute to the poor public opinion of nursing homes and, in turn, of the people who live and work in them. These reports also place nursing homes at a competitive disadvantage and may pose challenges to health delivery reform, including care integration across settings. © 2012 Milbank Memorial Fund.

  20. The Portrayal of Occupational Therapy and Occupational Science in Canadian Newspapers: A Content Analysis

    Directory of Open Access Journals (Sweden)

    Tsing-Yee (Emily Chai

    2016-05-01

    Full Text Available The primary goal of occupational therapy is to enable people to participate in the activities of everyday life. The demand for occupational therapists in Canada is expected to grow sharply at an annual growth rate of 3.2%, compared to 0.7% for all occupations. At the same time, it is believed by occupational therapists in Canada that the Canadian public does not understand the role of occupational therapy. Occupational science is an emerging basic science field that supports the practice of occupational therapy. Given that newspapers are one source the public uses to obtain information and that newspapers are seen to shape public opinions, the purpose of this study is to investigate how “occupational therapy” is covered in Canadian newspapers from the term’s first appearance in 1917 until 2016 and how “occupational science” is covered from the term’s first appearance in 1989 to 2016. We interrogated the findings through the lens of three non-newspaper sources—two academic journals: Canadian Journal of Occupational Therapy (CJOT and Journal of Occupational Science (JOS; and one Canadian magazine: Occupational Therapy Now (OTN. We found that medical terms were prevalent in the newspaper articles covering occupational therapy similar to the presence of medical terms in the CJOT and OTN. However, the newspapers missed contemporary shifts in occupational therapy as evident in the CJOT, OTN and JOS—such as the increased engagement with enablement, occupational justice and other occupational concepts. The newspapers also failed to portray the societal issues that occupational therapy engages with on behalf of and with their clients, and the newspapers did not cover many of the client groups of occupational therapy. Occupational science was only mentioned in n = 26 articles of the nearly 300 Canadian newspapers covered with no concrete content linked to occupational science. The scope of occupational therapy presented in Canadian

  1. How UK internet websites portray breast milk expression and breast pumps: a qualitative study of content.

    Science.gov (United States)

    McInnes, Rhona J; Arbuckle, Alix; Hoddinott, Pat

    2015-04-02

    Exclusive breastfeeding for six months is recommended but few parents achieve this; particularly younger and less well-educated mothers. Many parents introduce infant formula milk to manage feeding but describe a desire to express breastmilk alongside a lack of support or information. The Internet is highlighted as a key resource. This study aimed to examine UK websites on expressing breastmilk to identify key messages and how information is provided. We used search terms in Google to identify websites with information rich content on expressing breastmilk and breast pumps. Ten sites were purposively selected at two time points in 2013 and 2014 to represent 3 categories: commercial, NHS or 3(rd) sector (voluntary or not for profit). Each site was reviewed by two researchers, data and reflective analytical notes were uploaded into NVivo and thematic data analysis undertaken. Sites varied considerably in their design, use of images, videos, audio files, product placement and marketing opportunities. Three key themes emerged: depiction of expressing; reasons to express; and recommendations about expressing. Inconsistent and conflicting information was common within and between sites. Expressing was portrayed as similar to, but easier than, breastfeeding although at the same time difficult and requiring to be learned. Expressed breastmilk is promoted by mainly commercial sites as immediately available, although pumps were also presented as needing to be concealed, not heard or seen. Health benefits were the overarching reason for expressing. Although predicated on separation from the baby, commercial sites identified this as a positive choice while other sites focused on separation due to circumstance. Commercial sites emphasised restrictions related to breastfeeding, lack of sleep and bonding with the father and wider family. Non-commercial sites emphasised hand expression, with some not mentioning breast pumps. Practical information about starting expressing in

  2. South Experimental Oculina Research Reserve, Clelia Dive 610 2001 Digital Imagery - Captured from Videotapes taken during Submersible Dives to the Oculina Banks Deep Sea Coral Reefs (NODC Accession 0047190)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Digitial imagery, mpegs and jpegs, captured from mini-DV magnetic videotapes collected with an underwater 3-chip CCD color video camera, deployed from the research...

  3. Central Experimental Oculina Research Reserve, Clelia Dive 612 2001 Digital Imagery - Captured from Videotapes taken during Submersible Dives to the Oculina Banks Deep Sea Coral Reefs (NODC Accession 0047190)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Digitial imagery, mpegs and jpegs, captured from mini-DV magnetic videotapes collected with an underwater 3-chip CCD color video camera, deployed from the research...

  4. The effect of alcohol advertising, marketing and portrayal on drinking behaviour in young people: systematic review of prospective cohort studies.

    Science.gov (United States)

    Smith, Lesley A; Foxcroft, David R

    2009-02-06

    The effect of alcohol portrayals and advertising on the drinking behaviour of young people is a matter of much debate. We evaluated the relationship between exposure to alcohol advertising, marketing and portrayal on subsequent drinking behaviour in young people by systematic review of cohort (longitudinal) studies. studies were identified in October 2006 by searches of electronic databases, with no date restriction, supplemented with hand searches of reference lists of retrieved articles. Cohort studies that evaluated exposure to advertising or marketing or alcohol portrayals and drinking at baseline and assessed drinking behaviour at follow-up in young people were selected and reviewed. seven cohort studies that followed up more than 13,000 young people aged 10 to 26 years old were reviewed. The studies evaluated a range of different alcohol advertisement and marketing exposures including print and broadcast media. Two studies measured the hours of TV and music video viewing. All measured drinking behaviour using a variety of outcome measures. Two studies evaluated drinkers and non-drinkers separately. Baseline non-drinkers were significantly more likely to have become a drinker at follow-up with greater exposure to alcohol advertisements. There was little difference in drinking frequency at follow-up in baseline drinkers. In studies that included drinkers and non-drinkers, increased exposure at baseline led to significant increased risk of drinking at follow-up. The strength of the relationship varied between studies but effect sizes were generally modest. All studies controlled for age and gender, however potential confounding factors adjusted for in analyses varied from study to study. Important risk factors such as peer drinking and parental attitudes and behaviour were not adequately accounted for in some studies. data from prospective cohort studies suggest there is an association between exposure to alcohol advertising or promotional activity and

  5. Women and mass media: a critical and analytical study of the portrayal of Sudanese women in printed media.

    Science.gov (United States)

    Badri, A E; Osama, S

    1995-06-01

    This study examines how Sudanese women are portrayed in the mass media. Data are obtained from a content analysis of historical records of Sudanese daily newspapers and women's magazines and from surveys among female editors in print media. The following types of newspapers are reviewed: independent newspapers; papers for the Al-Umma Party, a communist party, a Bathist party, a Muslim Nationalist Islamic Front Party, and a National Union Democratic Party; and a current military government paper. Women's magazines are published by women. Articles focus on women as the main newsmakers, women's life issues, female authors, a female focus but a male author, and famous Sudanese women. 16 content themes are identified. Women were not extensively featured or photographed in either newspapers or magazines. The Al-Umma Party paper and Al-Sudan Al-Hadith paper (an independent paper) were the only two newspapers with at least 10 photos of women. Women were pictured as professionals, educated persons, and leaders. There were 17 female editors. These editors preferred an image of women as leaders, followed by productive workers. Only 11.76% believed that women's dual roles as producers and reproducers should be portrayed. Female editors did not want a special women's page. 52.94% (the largest percentage) preferred targeting women with substantial leadership abilities. 17.65% desired the portrayal of women as workers and housewives. 58.82% did not think that the mass media image changed behavior or attitudes, because most Sudanese women are illiterate. Women's issues in both newspapers and women's magazines were devoted to women's work, achievements, and needs. The authors recommend removal of obstacles to women's equal participation in the mass media and press and research on the effect of media images on women's self-perception and behavior.

  6. A content analysis of the portrayal of alcohol in televised music videos in New Zealand: changes over time.

    Science.gov (United States)

    Sloane, Kate; Wilson, Nick; Imlach Gunasekara, Fiona

    2013-01-01

    We aimed to: (i) document the extent and nature of alcohol portrayal in televised music videos in New Zealand in 2010; and (ii) assess trends over time by comparing with a similar 2005 sample. We undertook a content analysis for references to alcohol in 861 music videos shown on a youth-orientated television channel in New Zealand. This was compared with a sample in 2005 (564 music videos on the same channel plus sampling from two other channels). The proportion of alcohol content in the music videos was slightly higher in 2010 than for the same channel in the 2005 sample (19.5% vs. 15.7%) but this difference was not statistically significant. Only in the genre 'Rhythm and Blues' was the increase over time significant (P = 0.015). In both studies, the portrayal of alcohol was significantly more common in music videos where the main artist was international (not from New Zealand). Furthermore, in the music videos with alcohol content, at least a third of the time, alcohol was shown being consumed and the main artist was involved with alcohol. In only 2% (in 2005) and 4% (in 2010) of these videos was the tone explicitly negative towards alcohol. In both these studies, the portrayal of alcohol was relatively common in music videos. Nevertheless, there are various ways that policy makers can denormalise alcohol in youth-orientated media such as music videos or to compensate via other alcohol control measures such as higher alcohol taxes. © 2012 Australasian Professional Society on Alcohol and other Drugs.

  7. Adenosine triphosphate-binding cassette member A3 gene mutation in children from one family from Saudi Arabia.

    Science.gov (United States)

    Mukhtar, Gawahir Mohamed Ahmed; Al Otaibi, Wadha Hilal; Al-Mobaireek, Khalid Fahad Abdullah; Al-Saleh, Suhail

    2016-01-01

    Mutation in ABCA3, which is adenosine triphosphate-binding cassette member A3, a member of protein transporter family for phospholipids into the lamellar bodies during synthesis of surfactant, can cause lung disease related to surfactant dysfunction with autosomal recessive pattern. We reported three cases from same family with ABCA3 mutation, their gene, clinical course, and outcomes mentioning that one patient had successful lung transplantation, one started the process of the lung transplantation while the third one died during infancy. We concluded that the patients with ABCA3 gene mutations are increasing in numbers may be due to the availability of the genetic testing and high index of suspicion among physicians. Lung transplantation is the definitive treatment, but availability is limited in our region.

  8. Adenosine triphosphate-binding cassette member A3 gene mutation in children from one family from Saudi Arabia

    Directory of Open Access Journals (Sweden)

    Gawahir Mohamed Ahmed Mukhtar

    2016-01-01

    Full Text Available Mutation in ABCA3, which is adenosine triphosphate-binding cassette member A3, a member of protein transporter family for phospholipids into the lamellar bodies during synthesis of surfactant, can cause lung disease related to surfactant dysfunction with autosomal recessive pattern. We reported three cases from same family with ABCA3 mutation, their gene, clinical course, and outcomes mentioning that one patient had successful lung transplantation, one started the process of the lung transplantation while the third one died during infancy. We concluded that the patients with ABCA3 gene mutations are increasing in numbers may be due to the availability of the genetic testing and high index of suspicion among physicians. Lung transplantation is the definitive treatment, but availability is limited in our region.

  9. Isolated cerebellar variant of adrenoleukodystrophy with a de novo adenosine triphosphate-binding cassette D1 (ABCD1) gene mutation.

    Science.gov (United States)

    Kang, Joon Won; Lee, Sang Mi; Koo, Kyo Yeon; Lee, Young-Mock; Nam, Hyo Suk; Quan, Zhejiu; Kang, Hoon-Chul

    2014-07-01

    X-linked adrenoleukodystrophy (X-ALD) shows a wide range of phenotypic expression, but clinical presentation as an isolated lesion of the cerebellar white matter and dentate nuclei has not been reported. We report an unusual presentation of X-ALD only with an isolated lesion of the cerebellar white matter and dentate nuclei. The proband, a 37-year-old man presented with bladder incontinence, slurred speech, dysmetria in all limbs, difficulties in balancing, and gait ataxia. Brain magnetic resonance imaging showed an isolated signal change of white matter around the dentate nucleus in cerebellum. With high level of very long chain fatty acid, gene study showed a de novo mutation in exon 1 at nucleotide position c.277_296dup20 (p.Ala100Cysfs*10) of the adenosine triphosphate-binding cassette D1 gene. It is advised to consider X-ALD as a differential diagnosis in patients with isolated cerebellar degeneration symptoms.

  10. An Optimized GD2-Targeting Retroviral Cassette for More Potent and Safer Cellular Therapy of Neuroblastoma and Other Cancers.

    Directory of Open Access Journals (Sweden)

    Simon Thomas

    Full Text Available Neuroblastoma is the commonest extra cranial solid cancer of childhood. Despite escalation of treatment regimens, a significant minority of patients die of their disease. Disialoganglioside (GD2 is consistently expressed at high-levels in neuroblastoma tumors, which have been targeted with some success using therapeutic monoclonal antibodies. GD2 is also expressed in a range of other cancer but with the exception of some peripheral nerves is largely absent from non-transformed tissues. Chimeric Antigen Receptors (CARs are artificial type I proteins which graft the specificity of a monoclonal antibody onto a T-cell. Clinical data with early CAR designs directed against GD2 have shown some promise in Neuroblastoma. Here, we describe a GD2-targeting CAR retroviral cassette, which has been optimized for CAR T-cell persistence, efficacy and safety.

  11. A Study on the Measurement of the Pollution Level of Bacteria and Disinfection of Table and IP Cassette

    Energy Technology Data Exchange (ETDEWEB)

    Bae, Seok Hwan; Lee, Moo Sik; Lim, Chang Seon; Kim, Gha Jung [Koyang University, Koyang (Korea, Republic of)

    2008-09-15

    For the number of microbes and the pollution level of bacteriology of IP Cassette and Table by laboratories, after identifying the bacteria before and after using alcohol and tissue including disinfectant and statistically testing, this research was intended to provide the basic data for the prevention and the right disinfection guideline for infection management of hospitals in diagnostic radiology. The subject of this research was the general room of diagnostic radiology of a university hospital in Daejeon City. The research was conducted from Apr 5 to Apr 12, 2007. The number of microbes and the pollution level of bacteriology of IP Cassette and Table by laboratories were tested before and after using alcohol and tissue for disinfection including disinfectant. In order to collect specimens exactly, they were collected with the nurse who specialized in infection management of the hospital, and statistical processing was done with SPSS V13.0. To compare the results before and after using alcohol and tissue, T-test was implemented, and post-hoc test was conducted. Bacteria were detected in 19 cases of 24 subjects(79.2%), however, they were not detected in 5 cases(20.8%). 7 kinds of bacteria were detected as isolated bacteria, of which Methicillin Resistant coagulase-negative Staphylococci(MRCNS) were detected in 15 cases(62.5%), which was most, Methicillin Resistant Staphylococcus Aureus(MRSA) in 6 cases(16.7%), Enterococcus Faecium(EFM) in 5 cases(20.8%), Acinetobacter baumannii(ABA) in 2 cases(8.3%), and Bacillus sp, Coagulase-negative Staphylococci(CNS), and Enterococcus sp(ENT) in 1 case, respectively(4.2%). In all bacteria except ABA, Gram positive bacillia were detected in 30 cases(97%), and Gram negative bacilli were detected only in 1 case(3%). As for the kinds of bacteria and the number of groups before and after using 70% Alcohol by Groups, when the bacteria were identified after disinfecting IP Cassette and Table with 70% Alcohol, all the bacteria became

  12. Multicultural media portrayals and the changing demographic landscape: the psychosocial impact of television representations on the adolescent of color.

    Science.gov (United States)

    Berry, G L

    2000-08-01

    The developmental stage of adolescence can be a challenging period with the need for the young person to negotiate certain early psychosocial and physiologic developmental tasks. The developmental tasks that are common to most adolescents can include some unique social, economic, and educational experiences for minority youth because of the sociocultural construction of American society. This article explores some of the theories, concepts, and creative issues related to the psychosocial impact on adolescents of color and multicultural portrayals that appear on television and in other emerging media.

  13. Expression of Blf1-Stx B Gene Cassette in E. coli and Investigation Antibody Titer in Mice

    Directory of Open Access Journals (Sweden)

    Mehdi Masoudi Kerahroudi

    2017-02-01

    Full Text Available Background and Objectives: One of the ways to strengthen the effect of vaccines is using the adjuvant. STxB has a carrier and adjuvant role that we can fuse it with vaccine candidate antigens and produce efficient vaccines. BLF1 has a role in the pathogenesis and infection by Burkholderia pseudomallei, that can be studied when fused with stxB.The aim of this study expression blf1-stxB gene Cassette in E. coli and antibody production in mice. Methods:In this study the synthetic gene in pUC57 plasmid was purchased from Nedaye Fan COR. pUC57 plasmid containing blf1 with BamHI , SalI restriction enzyme sites was subcloned in pET28a(+-stxB expression vector and transformed into E. coli BL21 DE3. expression blf1-stxB gene Cassette was induced by IPTG. After extraction by affinity chromatography, the recombinant protein was injected four times to mice. Results: In this study, blf1-StxB cloned gene in pET28a(+ expression vector was approved by PCR and enzymatic analysis. Also recombinant protein confirmed by SDS-PAGE and Western blotting. Then antibody produced from the mice serum, were isolated and confirmed by ELISA. Conclusion: Given that BLF1 protein has the ability to stop protein synthesis and STxB has a carrier and adjuvant role, also with respect to antibody titers produced, it’s as a vaccine candidate against B. pseudomallei and Shigella dysenteriae.

  14. Ultrathin wear-resistant coatings for the tape bearing surface of thin-film magnetic heads for digital compact cassette

    Science.gov (United States)

    Zieren, V.; Dejongh, M.; Vangroenou, A. Broese; Vanzon, J. B. A.; Lasinski, P.; Theunissen, G. S. A. M.

    1994-03-01

    The introduction of the digital compact cassette (DCC) system into the consumer market imposed some severe requirements on the durability of the thin-film heads used in this system. The reliability of the head's record and playback functions has to be maintained under a wide variety of climatic conditions and tape types, as a consequence of the system's backward compatibility with the analog compact cassette. This paper describes the results of several accelerated wear tests as well as of durability tests against tape, which were set-up to find the best material and process conditions for a wear-resistant coating on the tape bearing surface of a DCC head. This ultrathin coating (less than 75 nm) appeared to be the best solution as a safeguard against severe pole-tip recession, which would cause an unacceptable distance loss in the recording system. The first material which showed a very high wear resistance was CrN. As CrN fulfils all requirements for wear and corrosion resistance in the home application, this material is presently used in all DCC home decks. However, under extremely abrasive conditions, such as may be encountered in a car environment (e.g. cold start at -20 C), CrN is surpassed by an even more wear-resistant material, called SPL (super protective layer). Therefore, SPL is preferred as a coating on thin-film heads for car stereo (as well as portable) DCC apparatus. Both materials are characterized by their specific wear performance, which has a superior impact on the electrical (record and playback) properties of the head during its lifetime.

  15. Computational prediction of CRISPR cassettes in gut metagenome samples from Chinese type-2 diabetic patients and healthy controls.

    Science.gov (United States)

    Mangericao, Tatiana C; Peng, Zhanhao; Zhang, Xuegong

    2016-01-11

    CRISPR has been becoming a hot topic as a powerful technique for genome editing for human and other higher organisms. The original CRISPR-Cas (Clustered Regularly Interspaced Short Palindromic Repeats coupled with CRISPR-associated proteins) is an important adaptive defence system for prokaryotes that provides resistance against invading elements such as viruses and plasmids. A CRISPR cassette contains short nucleotide sequences called spacers. These unique regions retain a history of the interactions between prokaryotes and their invaders in individual strains and ecosystems. One important ecosystem in the human body is the human gut, a rich habitat populated by a great diversity of microorganisms. Gut microbiomes are important for human physiology and health. Metagenome sequencing has been widely applied for studying the gut microbiomes. Most efforts in metagenome study has been focused on profiling taxa compositions and gene catalogues and identifying their associations with human health. Less attention has been paid to the analysis of the ecosystems of microbiomes themselves especially their CRISPR composition. We conducted a preliminary analysis of CRISPR sequences in a human gut metagenomic data set of Chinese individuals of type-2 diabetes patients and healthy controls. Applying an available CRISPR-identification algorithm, PILER-CR, we identified 3169 CRISPR cassettes in the data, from which we constructed a set of 1302 unique repeat sequences and 36,709 spacers. A more extensive analysis was made for the CRISPR repeats: these repeats were submitted to a more comprehensive clustering and classification using the web server tool CRISPRmap. All repeats were compared with known CRISPRs in the database CRISPRdb. A total of 784 repeats had matches in the database, and the remaining 518 repeats from our set are potentially novel ones. The computational analysis of CRISPR composition based contigs of metagenome sequencing data is feasible. It provides an efficient

  16. Who speaks for the climate now? Exploring portrayals of climate change through new/social media

    Science.gov (United States)

    Boykoff, M. T.

    2011-12-01

    Mass media stitch together formal science and policy with everyday activities in the public sphere. Many dynamic, contested and complex factors, along with non-nation state actors (or 'debate shapers'), contribute to how media outlets portray various facets of climate change. Against this backdrop, new and social media have become increasingly influential. The Pew Center Project for Excellence in Journalism has found that topics involving global warming have earned a much greater share of the news hole in new and social media (Internet weblogs, Twitter) than in traditional outlets (television, newspapers, radio), relative to other stories in those same media. This may be due in part to the flexibility and potentially infinite nature of the 'news hole' in new and social media, but may also mark the trends of diminishing traditional news room capabilities. Overall, new and social media have increasingly been harnessed in a variety of ways for communications about climate change around the world. However, with these shifts and developments come numerous questions. Among them: does increased visibility of climate change in new/social media translate to improved communication, or just more noise? Do these spaces provide opportunities for new forms of deliberative community regarding questions of climate mitigation and adaptation? Or has the content of this increased coverage shifted to polemics and arguments over measured traditional media analysis? In this more open space of content production, do new/social media provide more space for contrarian views to circulate? And through its interactivity, does increased consumption of news through new/social media further fragment a public discourse on climate mitigation and adaptation, through information silos where members of the public can stick to sources that help support their already held views? As new and social media representations of climate change demonstrate, the boundaries between who constitute 'authorized

  17. Identification and characterization of novel loss of function mutations in ATP-binding cassette transporter A1 in patients with low plasma high-density lipoprotein cholesterol

    NARCIS (Netherlands)

    Candini, C.; Schimmel, A. W.; Peter, J.; Bochem, A. E.; Holleboom, A. G.; Vergeer, M.; Dullaart, R. P. F.; Dallinga-Thie, G. M.; Hovingh, G. K.; Khoo, K. L.; Fasano, T.; Bocchi, L.; Calandra, S.; Kuivenhoven, J. A.; Motazacker, M. M.

    2010-01-01

    Objectives: The current literature provides little information on the frequency of mutations in the ATP-binding cassette transporter A1 (ABCA1) in patients with low high-density lipoprotein cholesterol (HDL) levels that are referred to the clinic. In 78 patients with low plasma levels of HDL

  18. A simplified multiplex PCR assay for fast and easy discrimination of globally distributed staphylococcal cassette chromosome mec types in meticillin-resistant Staphylococcus aureus

    NARCIS (Netherlands)

    E. Ghaznavi Rad (Ehsanollah); N.S. Mariana (Nor Shamsudin); Z. Sekawi (Zamberi); A.F. van Belkum (Alex); V. Neela (Vasanthakumari)

    2010-01-01

    textabstractA multiplex PCR assay was developed for the identification of major types and subtypes of staphylococcal cassette chromosome mec (SCCmec) in meticillin-resistant Staphylococcus aureus (MRSA) strains. The method uses a novel 9 valent multiplex PCR plus two primer pairs for S. aureus

  19. Endocrine Disruptors Differentially Target ATP-Binding Cassette Transporters in the Blood-Testis Barrier and Affect Leydig Cell Testosterone Secretion In Vitro

    NARCIS (Netherlands)

    Dankers, A.C.A.; Roelofs, M.J.; Piersma, A.H.; Sweep, F.C.; Russel, F.G.M.; Berg, M. van den; Duursen, M.B. van; Masereeuw, R.

    2013-01-01

    Endocrine-disrupting chemicals (EDCs) are considered to cause testicular toxicity primarily via interference with steroid hormone function. Alternatively, EDCs could possibly exert their effects by interaction with ATP-binding cassette (ABC) transporters that are expressed in the blood-testis

  20. Regulation of ATP-binding cassette transporters and cholesterol efflux by glucose in primary human monocytes and murine bone marrow-derived macrophages

    Science.gov (United States)

    Individuals with type 2 diabetes mellitus are at increased risk of developing atherosclerosis. This may be partially attributable to suppression of macrophage ATP-binding cassette (ABC) transporter mediated cholesterol efflux by sustained elevated blood glucose concentrations. Two models were used...