Arabidopsis thaliana as a tool to identify traits involved in Verticillium dahliae biocontrol by the olive root endophyte Pseudomonas fluorescens PICF7

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M. Mercedes eMaldonado-González

2015-04-01

Full Text Available The effective management of Verticillium wilts, diseases affecting many crops and caused by some species of the soil-borne fungus Verticillium, is problematic. The use of microbial antagonists to control these pathologies fits modern sustainable agriculture criteria. Pseudomonas fluorescens PICF7 is an endophytic bacterium isolated from olive roots with demonstrated ability to control Verticillium wilt of olive caused by the highly-virulent, defoliating (D pathotype of Verticillium dahliae Kleb. However, the study of the PICF7-V.dahliae-olive tripartite interaction poses difficulties because of the inherent characteristics of woody, long-living plants. To overcome these problems we explored the use of the model plant Arabidopsis thaliana. Results obtained in this study showed that: (i olive D and non-defoliating (ND V. dahliae pathotypes produce differential disease severity in A. thaliana plants; (ii strain PICF7 is able to colonize and persist in the A. thaliana rhizosphere but is not endophytic in Arabidopsis; and (iii strain PICF7 controls Verticillium wilt (VW in Arabidopsis. Additionally, as previously observed in olive, neither swimming motility nor siderophore production by PICF7 are required for VW control in A. thaliana, whilst cysteine auxotrophy decreased the effectiveness of PICF7. Moreover, when applied to the roots PICF7 controlled Botrytis cinerea infection in the leaves of Arabidopsis, suggesting that this strain is able to induce systemic resistance. Arabidopsis thaliana is therefore a suitable alternative to olive bioassays to unravel biocontrol traits involved in biological control of V. dahliae by P. fluorescens PICF7.

VdCYC8, encoding CYC8 glucose repression mediator protein, is required for microsclerotia formation and full virulence in Verticillium dahliae

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Verticillium dahliae is the primary causal agent for Verticillium wilt disease on a diverse array of economically important crops, including cotton. In previous research, we screened a T-DNA insertional mutant library of the highly virulent isolate Vd080 derived from cotton. In this study, the targ...

Nachweis von Verticillium dahliae an Pfefferminze (Mentha x piperita L.

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Gärber, Ute

2014-09-01

Full Text Available In Beständen von Pfefferminze wurden Krankheitssymptome beobachtet, die sich in Wuchshemmungen, Stängelverbräunungen, Blattchlorosen und Welke zeigten. Teilweise starben die Pflanzen bzw. einzelne Triebe ab. Als Ursache konnte Verticillium dahliae nachgewiesen werden. In einem Infektionsversuch im Gewächshaus wurden sieben Wochen alte Pfefferminzpflanzen der Sorte Multimentha durch Angießen mit einer Konidiensuspension (106 Konidien/ml Suspension inokuliert. Erste Krankheitssymptome erschienen acht Wochen nach Inokulation. Innerhalb kurzer Zeit nahm der Befall stark zu. Aus den kranken Pflanzenteilen wie Stängel und Blatt konnte der Erreger reisoliert werden.

Media for efficient generating nitrate- - nonutilizing (NIT) mutants of Verticillium dahliae, Colletotrichum gloeosporioides, Colletotrichum lindemuthianum and Fusarium oxysporum.

NARCIS (Netherlands)

Rataj-Guranowska, M.; Pieczul, K.; Nowak, E.; Hiemstra, J.A.; Drapikowska, M.

2001-01-01

The effect of several media amended wit potassium chlorate (1.5% and 6%) on generation of nit mutants, especially nit M mutants from Verticillium dahliae, Colletotrichum gloeosporioides, Colletotrichum lindemuthianum and Fusarium oxysporum were studied. For all species minimal medium with 6%

An Improved Single-Step Cloning Strategy Simplifies the Agrobacterium tumefaciens-Mediated Transformation (ATMT)-Based Gene-Disruption Method for Verticillium dahliae.

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Wang, Sheng; Xing, Haiying; Hua, Chenlei; Guo, Hui-Shan; Zhang, Jie

2016-06-01

The soilborne fungal pathogen Verticillium dahliae infects a broad range of plant species to cause severe diseases. The availability of Verticillium genome sequences has provided opportunities for large-scale investigations of individual gene function in Verticillium strains using Agrobacterium tumefaciens-mediated transformation (ATMT)-based gene-disruption strategies. Traditional ATMT vectors require multiple cloning steps and elaborate characterization procedures to achieve successful gene replacement; thus, these vectors are not suitable for high-throughput ATMT-based gene deletion. Several advancements have been made that either involve simplification of the steps required for gene-deletion vector construction or increase the efficiency of the technique for rapid recombinant characterization. However, an ATMT binary vector that is both simple and efficient is still lacking. Here, we generated a USER-ATMT dual-selection (DS) binary vector, which combines both the advantages of the USER single-step cloning technique and the efficiency of the herpes simplex virus thymidine kinase negative-selection marker. Highly efficient deletion of three different genes in V. dahliae using the USER-ATMT-DS vector enabled verification that this newly-generated vector not only facilitates the cloning process but also simplifies the subsequent identification of fungal homologous recombinants. The results suggest that the USER-ATMT-DS vector is applicable for efficient gene deletion and suitable for large-scale gene deletion in V. dahliae.

Agrobacterium-mediated transformation of verticillium dahliae with gfr gene to study cotton-pathogen interaction using a novel inoculation method

International Nuclear Information System (INIS)

Li, F.; Bibi, N.; Fan, K.; Wang, M.

2016-01-01

Verticillium dahliae is a soil-born fungal pathogen which causes Verticillium wilt in economically important crops including cotton. We conducted a study to monitor the interaction between the fungus and cotton. V. dahliae was transformed with the gene encoding green fluorescent protein. The gene can be constitutively expressed and fluorescence was clearly visible in both hyphae and spores. Due to heterogeneous gene insertion, the growth rate, colony morphology and pathogenicity of fungus transformants showed differences compared with corresponding wild type. Similarly, quantitative real-time PCR analysis also indicated significant differences in the gene expression among different V. dahliae transformants. To study cotton-pathogen interaction, we devised a novel inoculation method and developed a successful infection by keeping GFP-expressed mycelial plug along with aseptic cotton seedlings. After 6-day inoculation, the LSM microscopic image showed that the fungus rapidly formed a mycelial network on the surface of the stems and colonized into plant tissue, displayed an intercellular infection pattern. The early events during cotton colonization by V. dahliae can be successfully observed in 10 days including the plant growth period. Besides, pathological changes of seedlings like tissue discoloration, wilting, stem dehiscence and necrosis can be clearly observed without the influences of soil and other microbes. This inoculation method provides a rapid, effective and environmental friendly technique for the study of cotton-pathogen interaction and identification of resistant plant cultivars. (author)

Temporal impact of the vascular wilt pathogen Verticillium dahliae on tomato root proteome.

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Witzel, Katja; Buhtz, Anja; Grosch, Rita

2017-10-03

The soil-borne fungus Verticillium dahliae is the causal agent of wilting disease and affects a wide range of plant species worldwide. Here, we report on the time-resolved analysis of the tomato root proteome in response to fungal colonization. Tomato (Solanum lycopersicum cv. Hildares) was inoculated with V. dahliae at the two-leaf stage and roots were harvested at 7, 14 and 21 days post inoculation (dpi). In order to identify proteins related to the fungal spread at the different time points, a subsequent proteome analysis by two-dimensional differential gel electrophoresis (2D-DIGE) was conducted on samples from three independent experiments. Hierarchical clustering and k-means clustering of identified proteins distinguished early and late responses to fungal colonization. The results underline that plant defense and adaptation responses are timely coordinated. Proteins involved in oxidative stress were down-regulated at 7 dpi but induced 21 dpi indicating versatile reactive oxygen species signaling interacting with salicylic acid defence signaling at that stage of infection. Drought-stress proteins were induced at 21 dpi, reflecting the beginning of wilting symptoms. Notably, two proteins involved in energy-generating pathways were induced throughout all sampling dates and may reflect the increase in metabolic activity to maintain root growth and, concurrently, activate defense responses. Mounting of defense responses requires a substantial flux of carbon and nitrogen from primary to secondary metabolites. In-depth understanding of these key metabolic pathways required for growth and defense responses, especially at proteome level, will allow the development of breeding strategies for crops where Verticillium tolerance is absent. Our data show early and late responses of tomato root proteins towards pathogen infection and identify primary metabolism enzymes affected by V. dahliae. Those proteins represent candidates for plant improvement. Copyright © 2017

Identification of pathogenicity-related genes in the vascular wilt fungus verticillium dahliae by agrobacterium tumefaciens-mediated t-DNA insertional mutagenesis.

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Verticillium dahliae is the causal agent of vascular wilt in many economically important crops worldwide. Identification of genes that underpin pathogenicity or virulence may suggest targets for alternative control methods for this fungus. In this study, Agrobacterium tumefaciens-mediated transform...

Characterization of two homeodomain transcription factors with critical but distinct roles in virulence in the vascular pathogen Verticillium dahliae

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Vascular wilt caused by Verticillium dahliae is a destructive disease that represents a chronic economic problem for crop production worldwide. In this work, we characterized two new regulators of pathogenicity in this species. Vph1 (VDAG_06555) was identified in a candidate gene approach as a putat...

Fontes de resistência em acessos de Solanum (secção Lycopersicon a Verticillium dahliae raças 1 e 2 Sources of resistance to Verticillium dahliae races 1 and 2 in accessions of Solanum (section Lycopersicon

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Bruno Eduardo C de Miranda

2010-12-01

Full Text Available A murcha-de-verticílio (Verticillium dahliae é uma das doenças mais severas do tomateiro (Solanum lycopersicum L.. Duas raças fisiológicas de V. dahliae foram caracterizadas infectando o tomateiro e ambas estão presentes no Brasil. A estratégia de controle mais efetiva para isolados da raça 1 tem sido a utilização de cultivares resistentes em associação com práticas culturais. Entretanto, não existem cultivares comerciais de tomate resistentes à raça 2 do patógeno. Neste contexto, torna-se importante a identificação de novas fontes de resistência que sejam efetivas contra as duas raças de V. dahliae. Buscou-se fontes de resistência a isolados de V. dahliae (raças 1 e 2. Cem acessos de uma coleção de germoplasma contendo espécies cultivadas e selvagens de Solanum (secção Lycopersicon foram inicialmente inoculados pelo método de imersão de raízes com um isolado de V. dahliae raça 1 (5 mL de uma suspensão de 10(6 conídios/mL em condições de casa de vegetação. A avaliação foi feita aos 30 dias após a inoculação, usando uma escala de notas variando de 1 (planta sadia a 5 (planta morta. Foram considerados resistentes os acessos que apresentaram nota média entre 1 e 2. Um grupo composto por 38 acessos classificados como resistentes neste primeiro ensaio foi reavaliado para resistência a outros quatro isolados de V. dahliae pertencentes às raças 1 e 2. As cultivares Ponderosa e Floradade foram utilizadas como controles. O ensaio foi conduzido em casa de vegetação em delineamento experimental de blocos ao acaso em arranjo fatorial 5 x 40, com três repetições (vasos com quatro plantas. A avaliação foi feita baseada nos parâmetros epidemiológicos: período de incubação e índice de doença. Foram identificados acessos com resistência raça-específica e também com resistência a ambas as raças. Estes acessos podem ser indicados para futuros programas de melhoramento genético visando incorporar

Maintenance of sex-related genes and the co-occurrence of both mating types in Verticillium dahliae.

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Dylan P G Short

Full Text Available Verticillium dahliae is a cosmopolitan, soilborne fungus that causes a significant wilt disease on a wide variety of plant hosts including economically important crops, ornamentals, and timber species. Clonal expansion through asexual reproduction plays a vital role in recurring plant epidemics caused by this pathogen. The recent discovery of recombination between clonal lineages and preliminary investigations of the meiotic gene inventory of V. dahliae suggest that cryptic sex appears to be rare in this species. Here we expanded on previous findings on the sexual nature of V. dahliae. Only 1% of isolates in a global collection of 1120 phytopathogenic V. dahliae isolates contained the MAT1-1 idiomorph, whereas 99% contained MAT1-2. Nine unique multilocus microsatellite types comprised isolates of both mating types, eight of which were collected from the same substrate at the same time. Orthologs of 88 previously characterized sex-related genes from fungal model systems in the Ascoymycota were identified in the genome of V. dahliae, out of 93 genes investigated. Results of RT-PCR experiments using both mating types revealed that 10 arbitrarily chosen sex-related genes, including MAT1-1-1 and MAT1-2-1, were constitutively expressed in V. dahliae cultures grown under laboratory conditions. Ratios of non-synonymous (amino-acid altering to synonymous (silent substitutions in V. dahliae MAT1-1-1 and MAT1-2-1 sequences were indistinguishable from the ratios observed in the MAT genes of sexual fungi in the Pezizomycotina. Patterns consistent with strong purifying selection were also observed in 18 other arbitrarily chosen V. dahliae sex-related genes, relative to the patterns in orthologs from fungi with known sexual stages. This study builds upon recent findings from other laboratories and mounts further evidence for an ancestral or cryptic sexual stage in V. dahliae.

Phenological and phytochemical changes correlate with differential interactions of Verticillium dahliae with broccoli and cauliflower.

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Njoroge, S M C; Vallad, G E; Park, S-Y; Kang, S; Koike, S T; Bolda, M; Burman, P; Polonik, W; Subbarao, K V

2011-05-01

Cauliflower (Brassica oleracea var. botrytis subvar. cauliflora) is susceptible to wilt caused by Verticillium dahliae but broccoli (B. oleracea var. italica subvar. cyamosa) is not. Infection of broccoli and cauliflower by a green fluorescent protein-expressing isolate of V. dahliae was examined using epifluorescence and confocal laser-scanning microscopy to follow infection and colonization in relation to plant phenology. Plant glucosinolate, phenolic, and lignin contents were also assayed at 0, 4, 14, and 28 days postinoculation. V. dahliae consistently infected and colonized the vascular tissues of all cauliflower plants regardless of age at inoculation, with the pathogen ultimately appearing in the developing seed; however, colonization decreased with plant age. In broccoli, V. dahliae infected and colonized root and stem xylem tissues of plants inoculated at 1, 2, or 3 weeks postemergence. However, V. dahliae colonized only the root xylem and the epidermal and cortical tissues of broccoli plants inoculated at 4, 5, and 6 weeks postemergence. The frequency of reisolation of V. dahliae from the stems (4 to 22%) and roots (10 to 40%) of mature broccoli plants was lower than for cauliflower stems (25 to 64%) and roots (31 to 71%). The mean level of aliphatic glucosinolates in broccoli roots was 6.18 times higher than in the shoots and did not vary with age, whereas it was 3.65 times higher in cauliflower shoots than in the roots and there was a proportional increase with age. Indole glucosinolate content was identical in both cauliflower and broccoli, and both indole and aromatic glucosinolates did not vary with plant age in either crop. Qualitative differences in characterized glucosinolates were observed between broccoli and cauliflower but no differences were observed between inoculated and noninoculated plants for either broccoli or cauliflower. However, the phenolic and lignin contents were significantly higher in broccoli following inoculation than in

Deciphering the role of the phenylpropanoid metabolism in the tolerance of Capsicum annuum L. to Verticillium dahliae Kleb.

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Novo, Marta; Silvar, Cristina; Merino, Fuencisla; Martínez-Cortés, Teresa; Lu, Fachuang; Ralph, John; Pomar, Federico

2017-05-01

Verticillium dahliae is an economically relevant soilborne pathogen that causes vascular wilt in several crops, including pepper (Capsicum annuum). Fungal infection is usually visualized as a vascular browning, likely due to the onset of phenylpropanoid metabolism, which also seems to play a crucial role in the tolerance of some pepper varieties. In the current work, the potential function of distinct phenylpropanoid derivatives (suberin, lignin and phenolic compounds) in the pepper tolerance response against V. dahliae, was investigated. Histochemical and biochemical analyses ruled out suberin as a key player in the pepper-fungus interaction. However, changes observed in lignin composition and higher deposition of bound phenolics in infected stems seemed to contribute to the reinforcement of cell walls and the impairment of V. dahliae colonization. Most importantly, this is the first time that the accumulation of the hydroxycinnamic acid amide N-feruloyltyramine was reported in pepper stems in response to a vascular fungus. Fungitoxic activity for that hydroxycinnamate-tyramine conjugate was demonstrated as well. Copyright © 2017 Elsevier B.V. All rights reserved.

Screening of wild and cultivated Capsicum germplasm reveals new sources of Verticillium wilt resistance

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Verticillium wilt caused by Verticillium dahliae is an important soilborne disease of pepper (Capsicum species) worldwide. Most commercial pepper cultivars lack resistance to this pathogen. Our objective was to identify resistance to multiple V. dahliae isolates in wild and cultivated Capsicum acces...

The role of the β-1,6-endoglucanase gene vegB in physiology and virulence of Verticillium dahliae

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Lugard EBOIGBE

2014-05-01

Full Text Available The β-1,6-endoglucanase gene (vegB of Verticillium dahliae was isolated using a genome walking technique. Nucleotide and deduced amino acid sequences of the gene showed high identity with the PAN1 sequence deposited at the Verticillium genome database (Broad Institute, but significant differences in intron numbers and sites of insertion. Detailed in silico analysis, accompanied by sequencing of both genomic and cDNA, as well as RT-PCR experiments, provided the correct size of the gene and the exact number, length and positions of introns. The putative protein of this gene was compared with corresponding β-1,6-endoglucanases from other fungi, and sequences were used to construct a phylogenetic tree. A clear differentiation between enzymes derived from plant pathogenic and mycoparasitic fungi was observed, fully supported by bootstrap data. An internal fragment (1.2kb of vegB was used to disrupt the wild-type gene of a V. dahliae tomato race 2 strain, and the mutant strain, vegB-, was tested for pathogenicity on tomato plants. Results showed a small but constant reduction in disease symptoms only on eggplants for the vegB- strain in comparison with the wild type. Growth on minimal medium supplemented with different carbon sources showed reduced ability of the mutant to breakdown cellulose, whereas growth on glucose, pectin and sucrose was similar to the wild type.

In vitro analysis of the role of glucose oxidase from Talaromyces flavus in biocontrol of the plant pathogen Verticillium dahliae.

OpenAIRE

Stosz, S K; Fravel, D R; Roberts, D P

1996-01-01

Culture filtrates from Talaromyces flavus grown on glucose contained high levels of glucose oxidase activity, while culture filtrates from T. flavus grown on xylan contained negligible glucose oxidase activity. Culture filtrates from T-flavus grown on both media contained complex protein profiles. However, only culture filtrates from T. flavus grown on glucose inhibited germination of microsclerotia of Verticillium dahliae in in vitro inhibition assays. A polyclonal antiserum preparation, pAB...

Identification of miRNAs and Their Targets in Cotton Inoculated with Verticillium dahliae by High-Throughput Sequencing and Degradome Analysis

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Yujuan Zhang

2015-06-01

Full Text Available MicroRNAs (miRNAs are a group of endogenous small non-coding RNAs that play important roles in plant growth, development, and stress response processes. Verticillium wilt is a vascular disease in plants mainly caused by Verticillium dahliae Kleb., the soil-borne fungal pathogen. However, the role of miRNAs in the regulation of Verticillium defense responses is mostly unknown. This study aimed to identify new miRNAs and their potential targets that are involved in the regulation of Verticillium defense responses. Four small RNA libraries and two degradome libraries from mock-infected and infected roots of cotton (both Gossypium hirsutum L. and Gossypium barbadense L. were constructed for deep sequencing. A total of 140 known miRNAs and 58 novel miRNAs were identified. Among the identified miRNAs, many were differentially expressed between libraries. Degradome analysis showed that a total of 83 and 24 genes were the targets of 31 known and 14 novel miRNA families, respectively. Gene Ontology analysis indicated that many of the identified miRNA targets may function in controlling root development and the regulation of Verticillium defense responses in cotton. Our findings provide an overview of potential miRNAs involved in the regulation of Verticillium defense responses in cotton and the interactions between miRNAs and their corresponding targets. The profiling of these miRNAs lays the foundation for further understanding of the function of small RNAs in regulating plant response to fungal infection and Verticillium wilt in particular.

Late summer disease symptoms in western Washington red raspberry fields associated with co-occurrence of Phytophthora rubi, Verticillium dahliae, and Pratylenchus penetrans, but not Raspberry bushy dwarf virus

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60% of the $109 million processed by the U.S. red raspberry industry is in northern Washington. In 2012, late summer disease symptoms were observed in many raspberry fields. These symptoms were initially attributed to Verticillium dahliae, but other soilborne pathogens (Phytophthora rubi, Pratylench...

Verticillium dahliae-Arabidopsis Interaction Causes Changes in Gene Expression Profiles and Jasmonate Levels on Different Time Scales

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Sandra S. Scholz

2018-02-01

Full Text Available Verticillium dahliae is a soil-borne vascular pathogen that causes severe wilt symptoms in a wide range of plants. Co-culture of the fungus with Arabidopsis roots for 24 h induces many changes in the gene expression profiles of both partners, even before defense-related phytohormone levels are induced in the plant. Both partners reprogram sugar and amino acid metabolism, activate genes for signal perception and transduction, and induce defense- and stress-responsive genes. Furthermore, analysis of Arabidopsis expression profiles suggests a redirection from growth to defense. After 3 weeks, severe disease symptoms can be detected for wild-type plants while mutants impaired in jasmonate synthesis and perception perform much better. Thus, plant jasmonates have an important influence on the interaction, which is already visible at the mRNA level before hormone changes occur. The plant and fungal genes that rapidly respond to the presence of the partner might be crucial for early recognition steps and the future development of the interaction. Thus they are potential targets for the control of V. dahliae-induced wilt diseases.

Identification and Differentiation of Verticillium Species and V. longisporum Lineages by Simplex and Multiplex PCR Assays

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Inderbitzin, Patrik; Davis, R. Michael; Bostock, Richard M.; Subbarao, Krishna V.

2013-01-01

Accurate species identification is essential for effective plant disease management, but is challenging in fungi including Verticillium sensu stricto (Ascomycota, Sordariomycetes, Plectosphaerellaceae), a small genus of ten species that includes important plant pathogens. Here we present fifteen PCR assays for the identification of all recognized Verticillium species and the three lineages of the diploid hybrid V. longisporum. The assays were based on DNA sequence data from the ribosomal internal transcribed spacer region, and coding and non-coding regions of actin, elongation factor 1-alpha, glyceraldehyde-3-phosphate dehydrogenase and tryptophan synthase genes. The eleven single target (simplex) PCR assays resulted in amplicons of diagnostic size for V. alfalfae, V. albo-atrum, V. dahliae including V. longisporum lineage A1/D3, V. isaacii, V. klebahnii, V. nonalfalfae, V. nubilum, V. tricorpus, V. zaregamsianum, and Species A1 and Species D1, the two undescribed ancestors of V. longisporum. The four multiple target (multiplex) PCR assays simultaneously differentiated the species or lineages within the following four groups: Verticillium albo-atrum, V. alfalfae and V. nonalfalfae; Verticillium dahliae and V. longisporum lineages A1/D1, A1/D2 and A1/D3; Verticillium dahliae including V. longisporum lineage A1/D3, V. isaacii, V. klebahnii and V. tricorpus; Verticillium isaacii, V. klebahnii and V. tricorpus. Since V. dahliae is a parent of two of the three lineages of the diploid hybrid V. longisporum, no simplex PCR assay is able to differentiate V. dahliae from all V. longisporum lineages. PCR assays were tested with fungal DNA extracts from pure cultures, and were not evaluated for detection and quantification of Verticillium species from plant or soil samples. The DNA sequence alignments are provided and can be used for the design of additional primers. PMID:23823707

Starch Hydrolysis and Vessel Occlusion Related to Wilt Symptoms in Olive Stems of Susceptible Cultivars Infected by Verticillium dahliae

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Trapero, Carlos; Alcántara, Esteban; Jiménez, Jaime; Amaro-Ventura, María C.; Romero, Joaquín; Koopmann, Birger; Karlovsky, Petr; von Tiedemann, Andreas; Pérez-Rodríguez, Mario; López-Escudero, Francisco J.

2018-01-01

This study investigated starch content, amount of pathogen DNA and density of occluded vessels in healthy and Verticillium dahliae infected olive shoots and stems. Starch hydrolysis is considered a mechanism to refill xylem vessels that suffered cavitation by either, drought conditions or pathogen infections. The main objective of this work was to evaluate this mechanism in olive plants subjected to V. dahliae infection or to drought conditions, in order to know the importance of cavitation in the development of wilting symptoms. In initial experiments starch content in the shoots was studied in trees of cultivars differing in the level of resistance growing in fields naturally infested with V. dahliae. The starch content, esteemed by microscopic observation of stem transversal sections stained with lugol, decreased with the level of symptom severity. Results were confirmed in a new experiment developed with young plants of cultivars ‘Picual’ (highly susceptible), ‘Arbequina’ (moderately susceptible) and ‘Frantoio’ (resistant), growing in pots under greenhouse conditions, either inoculated or not with V. dahliae. In this experiment, the pathogen DNA content, quantified by real-time PCR, and the density of occluded vessels, recorded by microscopic observations of transversal sections stained with toluidine blue, were related to the symptoms severity caused by the pathogen. Finally, a drought experiment was established with young plants of the cultivar ‘Picual’ grown in pots under greenhouse conditions in order to compare the effects caused by water deficit with those caused by the pathogen infection. In both cases, results show that starch hydrolysis occurred, what indirectly evidence the importance of xylem cavitation in the development of the symptoms caused by V. dahliae but in the water stressed plants no vessel occlusion was detected. PMID:29445388

Starch Hydrolysis and Vessel Occlusion Related to Wilt Symptoms in Olive Stems of Susceptible Cultivars Infected by Verticillium dahliae

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Carlos Trapero

2018-01-01

Full Text Available This study investigated starch content, amount of pathogen DNA and density of occluded vessels in healthy and Verticillium dahliae infected olive shoots and stems. Starch hydrolysis is considered a mechanism to refill xylem vessels that suffered cavitation by either, drought conditions or pathogen infections. The main objective of this work was to evaluate this mechanism in olive plants subjected to V. dahliae infection or to drought conditions, in order to know the importance of cavitation in the development of wilting symptoms. In initial experiments starch content in the shoots was studied in trees of cultivars differing in the level of resistance growing in fields naturally infested with V. dahliae. The starch content, esteemed by microscopic observation of stem transversal sections stained with lugol, decreased with the level of symptom severity. Results were confirmed in a new experiment developed with young plants of cultivars ‘Picual’ (highly susceptible, ‘Arbequina’ (moderately susceptible and ‘Frantoio’ (resistant, growing in pots under greenhouse conditions, either inoculated or not with V. dahliae. In this experiment, the pathogen DNA content, quantified by real-time PCR, and the density of occluded vessels, recorded by microscopic observations of transversal sections stained with toluidine blue, were related to the symptoms severity caused by the pathogen. Finally, a drought experiment was established with young plants of the cultivar ‘Picual’ grown in pots under greenhouse conditions in order to compare the effects caused by water deficit with those caused by the pathogen infection. In both cases, results show that starch hydrolysis occurred, what indirectly evidence the importance of xylem cavitation in the development of the symptoms caused by V. dahliae but in the water stressed plants no vessel occlusion was detected.

The Verticillium dahliae SnodProt1-Like Protein VdCP1 Contributes to Virulence and Triggers the Plant Immune System

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Yi Zhang

2017-10-01

Full Text Available During pathogenic infection, hundreds of proteins that play vital roles in the Verticillium dahliae-host interaction are secreted. In this study, an integrated proteomic analysis of secreted V. dahliae proteins was performed, and a conserved secretory protein, designated VdCP1, was identified as a member of the SnodProt1 phytotoxin family. An expression analysis of the vdcp1 gene revealed that the transcript is present in every condition studied and displays elevated expression throughout the infection process. To investigate the natural role of VdCP1 in V. dahliae, two vdcp1 knockout mutants and their complementation strains were generated. Bioassays of these mutants revealed no obvious phenotypic differences from the wild-type (WT in terms of mycelial growth, conidial production or mycelial/spore morphology. However, compared with the WT, the vdcp1 knockout mutants displayed attenuated pathogenicity in cotton plants. Furthermore, treating plants with purified recombinant VdCP1 protein expressed in Pichia pastoris induced the accumulation of reactive oxygen species (ROS, expression of several defense-related genes, leakage of ion electrolytes, enhancement of defense-related enzyme activity and production of salicylic acid. Moreover, VdCP1 conferred resistance to Botrytis cinerea and Pseudomonas syringae pv. tabaci in tobacco and to V. dahliae in cotton. Further research revealed that VdCP1 possesses chitin-binding properties and that the growth of vdcp1 knockout mutants was more affected by treatments with chitinase, indicating that VdCP1 could protect V. dahliae cell wall from enzymatic degradation, which suggests an effector role of VdCP1 in infecting hosts.

Influence of soil type, cultivar and Verticillium dahliae on the structure of the root and rhizosphere soil fungal microbiome of strawberry.

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Nallanchakravarthula, Srivathsa; Mahmood, Shahid; Alström, Sadhna; Finlay, Roger D

2014-01-01

Sustainable management of crop productivity and health necessitates improved understanding of the ways in which rhizosphere microbial populations interact with each other, with plant roots and their abiotic environment. In this study we examined the effects of different soils and cultivars, and the presence of a soil-borne fungal pathogen, Verticillium dahliae, on the fungal microbiome of the rhizosphere soil and roots of strawberry plants, using high-throughput pyrosequencing. Fungal communities of the roots of two cultivars, Honeoye and Florence, were statistically distinct from those in the rhizosphere soil of the same plants, with little overlap. Roots of plants growing in two contrasting field soils had high relative abundance of Leptodontidium sp. C2 BESC 319 g whereas rhizosphere soil was characterised by high relative abundance of Trichosporon dulcitum or Cryptococcus terreus, depending upon the soil type. Differences between different cultivars were not as clear. Inoculation with the pathogen V. dahliae had a significant influence on community structure, generally decreasing the number of rhizosphere soil- and root-inhabiting fungi. Leptodontidium sp. C2 BESC 319 g was the dominant fungus responding positively to inoculation with V. dahliae. The results suggest that 1) plant roots select microorganisms from the wider rhizosphere pool, 2) that both rhizosphere soil and root inhabiting fungal communities are influenced by V. dahliae and 3) that soil type has a stronger influence on both of these communities than cultivar.

Breeding and genetics of lettuce for resistance against race 2 Verticillium wilt

Science.gov (United States)

Verticillium wilt, caused by Verticillium dahliae Kleb., is an economically important disease of lettuce in central coastal California. Most isolates of the pathogen detected in the Salinas Valley belong to race 1 for which complete resistance exists. However, adequate level of resistance is not ava...

The economics of managing Verticillium wilt, an imported disease in California lettuce

OpenAIRE

Carroll, Christine L; Carter, Colin A; Goodhue, Rachael E; Lin Lawell, C.-Y. Cynthia; Subbarao, Krishna V

2017-01-01

Verticillium dahliae is a soilborne fungus that is introduced to the soil via infested spinach seeds and that causes lettuce to be afflicted with Verticillium wilt. This disease has spread rapidly through the Salinas Valley, the prime lettuce production region of California. Verticillium wilt can be prevented or controlled by the grower by fumigating, planting broccoli, or not planting spinach. Because these control options require long-term investment for future gain, renters might not take ...

Ação antagônica e de metabólitos bioativos de Trichoderma spp. contra os patógenos Sclerotium rolfsii e Verticillium dahliae

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Carolina Oliveira Isaias

2014-03-01

Full Text Available O efeito antagônico exercido por fungos do gênero Trichoderma foi avaliado contra os fitopatógenos Sclerotium rolfsii e Verticillium dahliae, isolados de áster ornamental e morangueiro, respectivamente. Utilizaram-se 20 isolados do antagonista, pertencentes às espécies T. asperellum, T. hamatum, T. harzianum, T. koningiopsis, T. spirale e Trichoderma sp. O antagonismo foi verificado por meio de testes de culturas pareadas e produção de metabólitos bioativos pelo método de sobreposição de placas. A maioria dos isolados exibiu efeito inibitório sobre o crescimento micelial de S. rolfsii e V. dahliae quando comparado com as testemunhas. Excetuando-se os testes de produção de metabólitos não-voláteis, verificou-se maior ação inibitória contra V. dahliae. Mesmo os metabólitos não-voláteis termoestáveis apresentaram efeito inibitório contra ambos os patógenos testados, mostrando que os metabólitos secundários do antagonista exercem efeito significativo sobre essas espécies patogênicas.

Successful strategy for the selection of new strawberry-associated rhizobacteria antagonistic to Verticillium wilt.

Science.gov (United States)

Berg, G; Kurze, S; Buchner, A; Wellington, E M; Smalla, K

2000-12-01

In order to isolate and characterize new strawberry-associated bacteria antagonistic to the soil-borne pathogenic fungus Verticillium dahliae Kleb., rhizobacterial populations from two different strawberry species, Greenish Strawberry (Fragaria viridis) and Garden Strawberry (F. x ananassa) obtained after plating onto King's B and glycerol-arginine agar, were screened for in vitro antagonism toward V. dahliae. The proportion of isolates with antifungal activity determined in in vitro assay against V. dahliae was higher for the Garden Strawberry than for the Greenish Strawberry. From 300 isolates, 20 isolates with strong antifungal activity were selected characterized by physiological profiling and molecular fingerprinting methods. Diversity among the isolates was characterized with molecular fingerprints using amplified ribosomal DNA restriction analysis (ARDRA) and the more discriminating BOX-PCR fingerprint method. The physiological profiles were well correlated with molecular fingerprinting pattern analysis. Significant reduction of Verticillium wilt by bacterial dipping bath treatment was shown in the greenhouse and in fields naturally infested by V. dahliae. The relative increase of yield ranged from 117% (Streptomyces albidoflavus S1) to 344% (Pseudomonas fluorescens P10) in greenhouse trials, and 113% (Streptomyces albidoflavus S1) to 247% (Pseudomonas fluorescens P6) in field trials. Evaluation resulted in the selection of three effective biocontrol agents (Pseudomonas fluorescens P6, P10, and Streptomyces diastatochromogenes S9) antagonistic to the Verticillium wilt pathogen.

Proteomic analysis of the phytopathogenic soilborne fungus Verticillium dahliae reveals differential protein expression in isolates that differ in aggressiveness.

Science.gov (United States)

El-Bebany, Ahmed F; Rampitsch, Christof; Daayf, Fouad

2010-01-01

Verticillium dahliae is a soilborne fungus that causes a vascular wilt disease of plants and losses in a broad range of economically important crops worldwide. In this study, we compared the proteomes of highly (Vd1396-9) and weakly (Vs06-14) aggressive isolates of V. dahliae to identify protein factors that may contribute to pathogenicity. Twenty-five protein spots were consistently observed as differential in the proteome profiles of the two isolates. The protein sequences in the spots were identified by LC-ESI-MS/MS and MASCOT database searches. Some of the identified sequences shared homology with fungal proteins that have roles in stress response, colonization, melanin biosynthesis, microsclerotia formation, antibiotic resistance, and fungal penetration. These are important functions for infection of the host and survival of the pathogen in soil. One protein found only in the highly aggressive isolate was identified as isochorismatase hydrolase, a potential plant-defense suppressor. This enzyme may inhibit the production of salicylic acid, which is important for plant defense response signaling. Other sequences corresponding to potential pathogenicity factors were identified in the highly aggressive isolate. This work indicates that, in combination with functional genomics, proteomics-based analyses can provide additional insights into pathogenesis and potential management strategies for this disease.

The Ve-mediated resistance response of the tomato to Verticillium dahliae involves H2O2, peroxidase and lignins and drives PAL gene expression

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Merino Fuencisla

2010-10-01

Full Text Available Abstract Background Verticillium dahliae is a fungal pathogen that infects a wide range of hosts. The only known genes for resistance to Verticillium in the Solanaceae are found in the tomato (Solanum lycopersicum Ve locus, formed by two linked genes, Ve1 and Ve2. To characterize the resistance response mediated by the tomato Ve gene, we inoculated two nearly isogenic tomato lines, LA3030 (ve/ve and LA3038 (Ve/Ve, with V. dahliae. Results We found induction of H2O2 production in roots of inoculated plants, followed by an increase in peroxidase activity only in roots of inoculated resistant plants. Phenylalanine-ammonia lyase (PAL activity was also increased in resistant roots 2 hours after inoculation, while induction of PAL activity in susceptible roots was not seen until 48 hours after inoculation. Phenylpropanoid metabolism was also affected, with increases in ferulic acid, p-coumaric acid, vanillin and p-hydroxybenzaldehyde contents in resistant roots after inoculation. Six tomato PAL cDNA sequences (PAL1 - PAL6 were found in the SolGenes tomato EST database. RT-PCR analysis showed that these genes were expressed in all organs of the plant, albeit at different levels. Real-time RT-PCR indicated distinct patterns of expression of the different PAL genes in V. dahliae-inoculated roots. Phylogenetic analysis of 48 partial PAL cDNAs corresponding to 19 plant species grouped angiosperm PAL sequences into four clusters, suggesting functional differences among the six tomato genes, with PAL2 and PAL6 presumably involved in lignification, and the remaining PAL genes implicated in other biological processes. An increase in the synthesis of lignins was found 16 and 28 days after inoculation in both lines; this increase was greater and faster to develop in the resistant line. In both resistant and susceptible inoculated plants, an increase in the ratio of guaiacyl/syringyl units was detected 16 days after inoculation, resulting from the lowered amount

Fungal diversity associated with verticillium wilt of cotton

International Nuclear Information System (INIS)

Khaskheli, M.I.; Sun, J.L.; Li, F.

2014-01-01

The association of fungal diversity with Verticillium wilt is rarely known, which is important to know for the control of this detrimental disease. Our study is the preliminary attempt to find the associations of fungal diversity with Verticillium wilt and provides the baseline information for biological control. About 30 different fungi from soil and 23 from cotton plants were isolated and confirmed through molecular characterization. The colony forming unit (CFU)/g dry soil of fungi before and after planting cotton showed significant variation among all the fungi. The overall frequency of all fungi for soil after sowing was significantly higher than before sowing. A. alternata, F. equiseti, F. concentricum, A. flavus, F. proliferatum, and Chaetomium sp. associated with high resistance (Arcot-1) to Verticillium wilt, whereas, V. dahliae, A.niger and Paecilomyces sp., with high susceptible (Arcot-438) germplasm. However, T. basicola, C. ramotenellum and G. intermedia were isolated from both. Soil plating was comparatively easiest than soil dilution method for the determination of frequency percentage, however, later method is useful for the screening of single spore isolation. Most of the antagonistic species were screened from soil; nevertheless, Paecilomyces and Chaetomium spp. were screened from plant and soil. In vitro test of T. longibrachiatum. T. atroviride, Paecilomyces and T. viride showed the strongest efficacy against V. dahliae. These efficient bio-agents can be used as an effective tool for other future studies regarding to Verticillium wilt of cotton. (author)

Indigenous Pseudomonas spp. Strains from the Olive (Olea europaea L.) Rhizosphere as Effective Biocontrol Agents against Verticillium dahliae: From the Host Roots to the Bacterial Genomes

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Gómez-Lama Cabanás, Carmen; Legarda, Garikoitz; Ruano-Rosa, David; Pizarro-Tobías, Paloma; Valverde-Corredor, Antonio; Niqui, José L.; Triviño, Juan C.; Roca, Amalia; Mercado-Blanco, Jesús

2018-01-01

The use of biological control agents (BCA), alone or in combination with other management measures, has gained attention over the past decades, driven by the need to seek for sustainable and eco-friendly alternatives to confront plant pathogens. The rhizosphere of olive (Olea europaea L.) plants is a source of bacteria with potential as biocontrol tools against Verticillium wilt of olive (VWO) caused by Verticillium dahliae Kleb. A collection of bacterial isolates from healthy nursery-produced olive (cultivar Picual, susceptible to VWO) plants was generated based on morphological, biochemical and metabolic characteristics, chemical sensitivities, and on their in vitro antagonistic activity against several olive pathogens. Three strains (PIC25, PIC105, and PICF141) showing high in vitro inhibition ability of pathogens' growth, particularly against V. dahliae, were eventually selected. Their effectiveness against VWO caused by the defoliating pathotype of V. dahliae was also demonstrated, strain PICF141 being the rhizobacteria showing the best performance as BCA. Genotypic and phenotypic traits traditionally associated with plant growth promotion and/or biocontrol abilities were evaluated as well (e.g., phytase, xylanase, catalase, cellulase, chitinase, glucanase activities, and siderophore and HCN production). Multi-locus sequence analyses of conserved genes enabled the identification of these strains as Pseudomonas spp. Strain PICF141 was affiliated to the “Pseudomonas mandelii subgroup,” within the “Pseudomonas fluorescens group,” Pseudomonas lini being the closest species. Strains PIC25 and PIC105 were affiliated to the “Pseudomonas aeruginosa group,” Pseudomonas indica being the closest relative. Moreover, we identified P. indica (PIC105) for the first time as a BCA. Genome sequencing and in silico analyses allowed the identification of traits commonly associated with plant-bacteria interactions. Finally, the root colonization ability of these olive

Transcription factor VdCmr1 is required for pigment production, protection from UV irradiation, and regulates expression of melanin biosynthetic genes in Verticillium dahliae.

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Wang, Yonglin; Hu, Xiaoping; Fang, Yulin; Anchieta, Amy; Goldman, Polly H; Hernandez, Gustavo; Klosterman, Steven J

2018-04-01

Verticillium dahliae is a soilborne fungus that causes vascular wilt diseases on numerous plant species worldwide. The production of darkly melanized microsclerotia is crucial in the disease cycle of V. dahliae, as these structures allow for long-term survival in soil. Previously, transcriptomic and genomic analysis identified a cluster of genes in V. dahliae that encodes some dihydroxynaphthalene (DHN) melanin biosynthetic pathway homologues found in related fungi. In this study, we explored the roles of cluster-specific transcription factor VdCmr1, as well as two other genes within the cluster encoding a polyketide synthase (VdPKS1) and a laccase (VdLac1), enzymes at initial and endpoint steps in DHN melanin production. The results revealed that VdCmr1 and VdPKS1 are required for melanin production, but neither is required for microsclerotia production. None of the three genes were required for pathogenesis on tobacco and lettuce. Exposure of ΔVdCmr1 and wild-type strains to UV irradiation, or to high temperature (40 °C), revealed an approx. 50 % reduction of survival in the ΔVdCmr1 strain, relative to the wild-type strain, in response to either condition. Expression profiles revealed that expression of some melanin biosynthetic genes are in part dependent on VdCmr1. Combined data indicate VdCmr1 is a key regulator of melanin biosynthesis, and that via regulation of melanogenesis, VdCmr1 affects survival of V. dahliae in response to abiotic threats. We conclude with a model showing regulation of VdCmr1 by a high osmolarity glycerol response (Hog)-type MAP kinase pathway.

Heterologous Expression of the Cotton NBS-LRR Gene GbaNA1 Enhances Verticillium Wilt Resistance in Arabidopsis

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Nan-Yang Li

2018-02-01

Full Text Available Verticillium wilt caused by Verticillium dahliae results in severe losses in cotton, and is economically the most destructive disease of this crop. Improving genetic resistance is the cleanest and least expensive option to manage Verticillium wilt. Previously, we identified the island cotton NBS-LRR-encoding gene GbaNA1 that confers resistance to the highly virulent V. dahliae isolate Vd991. In this study, we expressed cotton GbaNA1 in the heterologous system of Arabidopsis thaliana and investigated the defense response mediated by GbaNA1 following inoculations with V. dahliae. Heterologous expression of GbaNA1 conferred Verticillium wilt resistance in A. thaliana. Moreover, overexpression of GbaNA1 enabled recovery of the resistance phenotype of A. thaliana mutants that had lost the function of GbaNA1 ortholog gene. Investigations of the defense response in A. thaliana showed that the reactive oxygen species (ROS production and the expression of genes associated with the ethylene signaling pathway were enhanced significantly following overexpression of GbaNA1. Intriguingly, overexpression of the GbaNA1 ortholog from Gossypium hirsutum (GhNA1 in A. thaliana did not induce the defense response of ROS production due to the premature termination of GhNA1, which lacks the encoded NB-ARC and LRR motifs. GbaNA1 therefore confers Verticillium wilt resistance in A. thaliana by the activation of ROS production and ethylene signaling. These results demonstrate the functional conservation of the NBS-LRR-encoding GbaNA1 in a heterologous system, and the mechanism of this resistance, both of which may prove valuable in incorporating GbaNA1-mediated resistance into other plant species.

Effect of solarization with fresh chicken manure on verticillium wilt ...

African Journals Online (AJOL)

The present study was carried out to evaluate the effect of reducing wilt disease through the medium of fresh chicken manure (FCM) mixed with soil before solarized and then artificial Verticillium dahliae (V.d) inoculation on yield of eggplant (Solanum melongena L.) under field conditions. According to the splitplot design, ...

The economics of managing Verticillium wilt, an imported disease in California lettuce

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Christine L. Carroll

2017-09-01

Full Text Available Verticillium dahliae is a soilborne fungus that is introduced to the soil via infested spinach seeds and that causes lettuce to be afflicted with Verticillium wilt. This disease has spread rapidly through the Salinas Valley, the prime lettuce production region of California. Verticillium wilt can be prevented or controlled by the grower by fumigating, planting broccoli, or not planting spinach. Because these control options require long-term investment for future gain, renters might not take the steps needed to control Verticillium wilt. Verticillium wilt can also be prevented or controlled by a spinach seed company through testing and cleaning the spinach seeds. However, seed companies are unwilling to test or clean spinach seeds, as they are not affected by this disease. We discuss our research on the externalities that arise with renters, and between seed companies and growers, due to Verticillium wilt. These externalities have important implications for the management of Verticillium wilt in particular, and for the management of diseases in agriculture in general.

The ascomycete Verticillium longisporum is a hybrid and a plant pathogen with an expanded host range.

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Patrik Inderbitzin

Full Text Available Hybridization plays a central role in plant evolution, but its overall importance in fungi is unknown. New plant pathogens are thought to arise by hybridization between formerly separated fungal species. Evolution of hybrid plant pathogens from non-pathogenic ancestors in the fungal-like protist Phytophthora has been demonstrated, but in fungi, the most important group of plant pathogens, there are few well-characterized examples of hybrids. We focused our attention on the hybrid and plant pathogen Verticillium longisporum, the causal agent of the Verticillium wilt disease in crucifer crops. In order to address questions related to the evolutionary origin of V. longisporum, we used phylogenetic analyses of seven nuclear loci and a dataset of 203 isolates of V. longisporum, V. dahliae and related species. We confirmed that V. longisporum was diploid, and originated three different times, involving four different lineages and three different parental species. All hybrids shared a common parent, species A1, that hybridized respectively with species D1, V. dahliae lineage D2 and V. dahliae lineage D3, to give rise to three different lineages of V. longisporum. Species A1 and species D1 constituted as yet unknown taxa. Verticillium longisporum likely originated recently, as each V. longisporum lineage was genetically homogenous, and comprised species A1 alleles that were identical across lineages.

Formation of microsclerotia of Verticillium dahliae on various crops.

NARCIS (Netherlands)

Mol, L.

1995-01-01

In 2 pot experiments, potato (cultivars Element, Mirka, Ostara and Astarte), pea, sugarbeet, onion, flax, spring barley, faba beans, spring wheat and spring rape were inoculated with V. dahliae by root dipping or by growing the plants in artificially infested soil. In both treatments the dry matter

Dynamics of Verticillium species microsclerotia in field soils in response to fumigation, cropping patterns, and flooding

Science.gov (United States)

Many soil-inhabiting fungi are capable of surviving the dynamic soil microenvironment through the formation of resilient resting structures, such as thick-walled spores, melanized hyphae, and sclerotia. Verticillium dahliae is a soil-inhabiting, economically significant plant pathogenic fungus that ...

Comparative genomics reveals cotton-specific virulence factors in flexible genomic regions in Verticillium dahliae and evidence of horizontal gene transfer from Fusarium.

Science.gov (United States)

Chen, Jie-Yin; Liu, Chun; Gui, Yue-Jing; Si, Kai-Wei; Zhang, Dan-Dan; Wang, Jie; Short, Dylan P G; Huang, Jin-Qun; Li, Nan-Yang; Liang, Yong; Zhang, Wen-Qi; Yang, Lin; Ma, Xue-Feng; Li, Ting-Gang; Zhou, Lei; Wang, Bao-Li; Bao, Yu-Ming; Subbarao, Krishna V; Zhang, Geng-Yun; Dai, Xiao-Feng

2018-01-01

Verticillium dahliae isolates are most virulent on the host from which they were originally isolated. Mechanisms underlying these dominant host adaptations are currently unknown. We sequenced the genome of V. dahliae Vd991, which is highly virulent on its original host, cotton, and performed comparisons with the reference genomes of JR2 (from tomato) and VdLs.17 (from lettuce). Pathogenicity-related factor prediction, orthology and multigene family classification, transcriptome analyses, phylogenetic analyses, and pathogenicity experiments were performed. The Vd991 genome harbored several exclusive, lineage-specific (LS) genes within LS regions (LSRs). Deletion mutants of the seven genes within one LSR (G-LSR2) in Vd991 were less virulent only on cotton. Integration of G-LSR2 genes individually into JR2 and VdLs.17 resulted in significantly enhanced virulence on cotton but did not affect virulence on tomato or lettuce. Transcription levels of the seven LS genes in Vd991 were higher during the early stages of cotton infection, as compared with other hosts. Phylogenetic analyses suggested that G-LSR2 was acquired from Fusarium oxysporum f. sp. vasinfectum through horizontal gene transfer. Our results provide evidence that horizontal gene transfer from Fusarium to Vd991 contributed significantly to its adaptation to cotton and may represent a significant mechanism in the evolution of an asexual plant pathogen. © 2017 The Authors. New Phytologist © 2017 New Phytologist Trust.

Real-Time PCR Detection and QUantification of Soilborne Fungal Pathogens : the Case of Rosellinia necatrix, Phytophthora nicotianae, P. citrophthora and Verticillium dahliae

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L. Schena

2004-08-01

Full Text Available Conventional and Scorpion primers were designed from the ITS regions to identify Rosellinia necatrix, Phytophthora nicotianae, and P. citrophthora and from the IGS regions to identify Verticillium dahliae and V. alboatrum. Specificity of primers and probes was assessed using genomic DNA from a large number of fungi from several hosts and by means of BLAST analyses, to exclude the presence of similar sequences in other micro-organisms among available DNA databases (GenBank. Simple and rapid procedures for DNA extraction from naturally infected matrices (soils, roots, bark, and/or woody tissues were utilised to yield DNA of a purity and quality suitable for PCR assays. Combining these protocols with a double amplification (nested Scorpion-PCR, the real-time detection of these pathogens was possible from naturally infested soils and from infected citrus roots (P. nicotianae and P. citrophthora, from the roots and bark of stone fruits and olive (R. necatrix and from olive branches (V. dahliae. For target pathogens, the limit of detection was 1 pg µl-1 in Scorpion-PCR and 1 fg µl-1 in nested Scorpion-PCR. High and significant correlations between pathogen propagule concentrations and real-time PCR cycle thresholds (Ct were obtained. Moreover, specific tests with R. necatrix seem to indicate that its DNA is quite rapidly degraded in the soil, excluding the risk of false positives due to the presence of dead cells.

Management of Soilborne Diseases in Strawberry Using Vegetable Rotations

OpenAIRE

Subbarao, Krishna V

2007-01-01

The influence of crop rotation on soilborne diseases and yield of strawberry (Fragaria × ananassa) was determined at a site infested with Verticillium dahliae microsclerotia and at another with no known history of V. dahliae infestation during 1997 to 2000. The rotations studied at the V. dahliae-infested site were (i) broccoli-broccoli-strawberry, (ii) Brussels sproutsstrawberry, and (iii) lettuce-lettuce-strawberry; the treatments at the site with no history of V. dahliae were (i) broccoli-...

SSH reveals a linkage between a senescence-associated protease and Verticillium wilt symptom development in lettuce (Lactuca sativa)

Science.gov (United States)

Suppression subtractive hybridization (SSH) was employed to identify lettuce (Lactuca sativa) genes that are differentially expressed in symptomatic leaves infected with Verticillium dahliae. Genes expressed only in symptomatic leaves included those with homology to pathogenesis-related (PR) protei...

Arbuscular mycorrhizal fungi (AMF) as bio protector agents against wilt induced by Verticillium spp. in pepper

Energy Technology Data Exchange (ETDEWEB)

Goicoechea, N.; Garmendia, I.; Sanchez-Diaz, M.; Aguirreolea, J.

2010-07-01

Verticillium dahliae Kleb. is a vascular pathogen that alters water status and growth of pepper plants and causes drastic reductions in yield. Its control is difficult because it can survive in field soil for several years. The application of arbuscular mycorrhizal fungi (AMF) as bio protector agents against V. dahliae is an alternative to the use of chemicals which, in addition, is more respectful with the environment. The establishment of the mutualistic association of plant roots and AMF involves a continuous cellular and molecular dialogue between both symbionts that includes the pre activation of plant defense responses that may enhance the resistance or tolerance of mycorrhizal plants to soil-borne pathogens. Some AMF can improve the resistance of Capsicum annuum L. against V. dahliae. This is especially relevant for pepper cultivars (i.e. cv. Piquillo) that exhibit high susceptibility to this pathogen. Compared with non-mycorrhizal plants, mycorrhizal pepper can exhibit more balanced antioxidant metabolism in leaves along the first month after pathogen inoculation, which may contribute to delay both the development of disease symptoms and the decrease of photosynthesis in Verticillium-inoculated plants with the subsequent benefit for yield. In stems, mycorrhizal pepper show earlier and higher deposition of lignin in xylem vessels than non mycorrhizal plants, even in absence of the pathogen. Moreover, AMF can induce new isoforms of acidic chitinases and superoxide dismutase in roots. Mycorrhizal-specific induction of these enzymatic activities together with enhanced peroxidase and phenylalanine ammonia-lyase in roots may also be involved in the bio protection of Verticillium-induced wilt in pepper by AMF. (Author) 81 refs.

A model for multiseasonal spread of verticillium wilt of lettuce.

Science.gov (United States)

Wu, B M; Subbarao, K V

2014-09-01

Verticillium wilt, caused by Verticillium dahliae, is a destructive disease in lettuce, and the pathogen is seedborne. Even though maximum seed infestation rates of lettuce seed lots, it is necessary to establish acceptable contamination thresholds to prevent introduction and establishment of the pathogen in lettuce production fields. However, introduction of inoculum into lettuce fields for experimental purposes to determine its long term effects is undesirable. Therefore, we constructed a simulation model to study the spread of Verticillium wilt following pathogen introduction from seed. The model consists of four components: the first for simulating infection of host plants, the second for simulating reproduction of microsclerotia on diseased plants, the third for simulating the survival of microsclerotia, and the fourth for simulating the dispersal of microsclerotia. The simulation results demonstrated that the inoculum density-disease incidence curve parameters and the dispersal gradients affect disease spread in the field. Although a steep dispersal gradient facilitated the establishment of the disease in a new field with a low inoculum density, a long-tail gradient allowed microsclerotia to be dispersed over greater distances, promoting the disease spread in fields with high inoculum density. The simulation results also revealed the importance of avoiding successive lettuce crops in the same field, reducing survival rate of microsclerotia between crops, and the need for breeding resistance against V. dahliae in lettuce cultivars to lower the number of microsclerotia formed on each diseased plant. The simulation results, however, suggested that, even with a low seed infestation rate, the pathogen would eventually become established if susceptible lettuce cultivars were grown consecutively in the same field for many years. A threshold for seed infestation can be established only when two of the three drivers of the disease-(i) low microsclerotia production per

Improvement of Verticillium Wilt Resistance by Applying Arbuscular Mycorrhizal Fungi to a Cotton Variety with High Symbiotic Efficiency under Field Conditions

Science.gov (United States)

Zhang, Qiang; Gao, Xinpeng; Ren, Yanyun; Ding, Xinhua; Qiu, Jiajia; Li, Ning; Zeng, Fanchang

2018-01-01

Arbuscular mycorrhizal fungi (AMF) play an important role in nutrient cycling processes and plant stress resistance. To evaluate the effect of Rhizophagus irregularis CD1 on plant growth promotion (PGP) and Verticillium wilt disease, the symbiotic efficiency of AMF (SEA) was first investigated over a range of 3% to 94% in 17 cotton varieties. The high-SEA subgroup had significant PGP effects in a greenhouse. From these results, the highest-SEA variety of Lumian 1 was selected for a two-year field assay. Consistent with the performance from the greenhouse, the AMF-mediated PGP of Lumian 1 also produced significant results, including an increased plant height, stem diameter, number of petioles, and phosphorus content. Compared with the mock treatment, AMF colonization obviously inhibited the symptom development of Verticillium dahliae and more strongly elevated the expression of pathogenesis-related genes and lignin synthesis-related genes. These results suggest that AMF colonization could lead to the mycorrhiza-induced resistance (MIR) of Lumian 1 to V. dahliae. Interestingly, our results indicated that the AMF endosymbiont could directly inhibit the growth of phytopathogenic fungi including V. dahliae by releasing undefined volatiles. In summary, our results suggest that stronger effects of AMF application result from the high-SEA. PMID:29342876

Improvement of Verticillium Wilt Resistance by Applying Arbuscular Mycorrhizal Fungi to a Cotton Variety with High Symbiotic Efficiency under Field Conditions

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Qiang Zhang

2018-01-01

Full Text Available Arbuscular mycorrhizal fungi (AMF play an important role in nutrient cycling processes and plant stress resistance. To evaluate the effect of Rhizophagus irregularis CD1 on plant growth promotion (PGP and Verticillium wilt disease, the symbiotic efficiency of AMF (SEA was first investigated over a range of 3% to 94% in 17 cotton varieties. The high-SEA subgroup had significant PGP effects in a greenhouse. From these results, the highest-SEA variety of Lumian 1 was selected for a two-year field assay. Consistent with the performance from the greenhouse, the AMF-mediated PGP of Lumian 1 also produced significant results, including an increased plant height, stem diameter, number of petioles, and phosphorus content. Compared with the mock treatment, AMF colonization obviously inhibited the symptom development of Verticillium dahliae and more strongly elevated the expression of pathogenesis-related genes and lignin synthesis-related genes. These results suggest that AMF colonization could lead to the mycorrhiza-induced resistance (MIR of Lumian 1 to V. dahliae. Interestingly, our results indicated that the AMF endosymbiont could directly inhibit the growth of phytopathogenic fungi including V. dahliae by releasing undefined volatiles. In summary, our results suggest that stronger effects of AMF application result from the high-SEA.

Fosfito de potássio na indução de resistência a Verticillium dahliae Kleb., em mudas de cacaueiro (Theobroma cacao L. Effect of potassium phosphite on the induction of resistance in cocoa seedlings (Theobroma cacao L. against Verticillium dahliae Kleb

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Pedro Martins Ribeiro Júnior

2006-08-01

Full Text Available Há vários relatos da utilização de fosfitos no controle de doenças de plantas, por meio de ação direta, antifúngica e indireta por indução de resistência. Essa atuação como indutor é questionada, não sendo encontradas, em muitos desses trabalhos, evidências de respostas de defesa ativadas pelos sais de fosfito. Com o presente trabalho, objetivou-se estudar o efeito de doses (0,62; 1,25; 2,5 e 5 mL.L-1 de água de fosfito de potássio na indução de resistência em mudas de cacaueiro a V. dahliae, além de investigar os possíveis mecanismos envolvidos na resposta de defesa. Foram realizados experimentos no Laboratório de Fisiologia do Parasitismo e casa-de-vegetação do Departamento de Fitopatologia UFLA. A aplicação foliar do fosfito foi realizada 7 dias antes das inoculações e as avaliações de severidade foram realizadas aos 20, 30, 40, 50 e 60 dias após a inoculação. Foi realizado também um experimento para verificar o efeito tóxico direto e outro para avaliar a atividade das enzimas peroxidases e polifenoloxidases e a concentração de lignina. O tratamento com o fosfito de potássio (1,25 mL.L-1 de água proporcionou 10% de redução na área abaixo da curva de progresso da severidade da murcha-de-Verticillium (AACPD, 60 dias após aplicação foliar, não diferindo de nenhuma das doses, nem da testemunha. Todas as doses utilizadas apresentaram efeito fungitóxico, inibindo a germinação de V. dahliae. A aplicação do fosfito de potássio (1,25 mL.L-1 de água não induziu aumento na atividade das enzimas peroxidases e polifenoloxidases em relação à testemunha. Plantas tratadas com esse produto apresentaram um pequeno incremento na concentração de lignina, não apresentando diferença significativa da testemunha absoluta.There are several reports concerning with the use of phosphites for controlling of plant diseases, through direct action, antifungal, and indirect, by resistance induction. The

The Three Lineages of the Diploid Hybrid Verticillium longisporum Differ in Virulence and Pathogenicity.

Science.gov (United States)

Novakazi, Fluturë; Inderbitzin, Patrik; Sandoya, German; Hayes, Ryan J; von Tiedemann, Andreas; Subbarao, Krishna V

2015-05-01

Verticillium longisporum is an economically important vascular pathogen of Brassicaceae crops in different parts of the world. V. longisporum is a diploid hybrid that consists of three different lineages, each of which originated from a separate hybridization event between two different sets of parental species. We used 20 isolates representing the three V. longisporum lineages and the relative V. dahliae, and performed pathogenicity tests on 11 different hosts, including artichoke, cabbage, cauliflower, cotton, eggplant, horseradish, lettuce, linseed, oilseed rape (canola), tomato, and watermelon. V. longisporum was overall more virulent on the Brassicaceae crops than V. dahliae, which was more virulent than V. longisporum across the non-Brassicaceae crops. There were differences in virulence between the three V. longisporum lineages. V. longisporum lineage A1/D1 was the most virulent lineage on oilseed rape, and V. longisporum lineage A1/D2 was the most virulent lineage on cabbage and horseradish. We also found that on the non-Brassicaceae hosts eggplant, tomato, lettuce, and watermelon, V. longisporum was more or equally virulent than V. dahliae. This suggests that V. longisporum may have a wider potential host range than currently appreciated.

Allelopathy of root exudates from different resistant eggplants to ...

African Journals Online (AJOL)

Three eggplant cultivars were inoculated with Verticillium dahliae Kleb. to assess their resistance to Verticillium wilt. Solanum tor was resistant, “Liyuanziqie” was tolerant, and “Xi'anlvqie” susceptible. The disease incidence and disease index of Verticillium wilt and the amount of V. dahliae in rhizospheric soil, variation of ...

Transposable elements in phytopathogenic Verticillium spp.: insights into genome evolution and inter- and intra-specific diversification

Directory of Open Access Journals (Sweden)

Amyotte Stefan G

2012-07-01

Full Text Available Abstract Background Verticillium dahliae (Vd and Verticillium albo-atrum (Va are cosmopolitan soil fungi causing very disruptive vascular diseases on a wide range of crop plants. To date, no sexual stage has been identified in either microorganism suggesting that somatic mutation is a major force in generating genetic diversity. Whole genome comparative analysis of the recently sequenced strains VdLs.17 and VaMs.102 revealed that non-random insertions of transposable elements (TEs have contributed to the generation of four lineage-specific (LS regions in VdLs.17. Results We present here a detailed analysis of Class I retrotransposons and Class II “cut-and-paste” DNA elements detected in the sequenced Verticillium genomes. We report also of their distribution in other Vd and Va isolates from various geographic origins. In VdLs.17, we identified and characterized 56 complete retrotransposons of the Gypsy-, Copia- and LINE-like types, as well as 34 full-length elements of the “cut-and-paste” superfamilies Tc1/mariner, Activator and Mutator. While Copia and Tc1/mariner were present in multiple identical copies, Activator and Mutator sequences were highly divergent. Most elements comprised complete ORFs, had matching ESTs and showed active transcription in response to stress treatment. Noticeably, we found evidences of repeat-induced point mutation (RIP only in some of the Gypsy retroelements. While Copia-, Gypsy- and Tc1/mariner-like transposons were prominent, a large variation in presence of the other types of mobile elements was detected in the other Verticillium spp. strains surveyed. In particular, neither complete nor defective “cut-and-paste” TEs were found in VaMs.102. Conclusions Copia-, Gypsy- and Tc1/mariner-like transposons are the most wide-spread TEs in the phytopathogens V. dahliae and V. albo-atrum. In VdLs.17, we identified several retroelements and “cut-and-paste” transposons still potentially active. Some of these

The endophyte Verticillium Vt305 protects cauliflower against Verticillium wilt.

Science.gov (United States)

Tyvaert, L; França, S C; Debode, J; Höfte, M

2014-06-01

To investigate the interaction between cauliflower and the isolate VerticilliumVt305, obtained from a field suppressive to Verticillium wilt of cauliflower, and to evaluate the ability of VerticilliumVt305 to control Verticillium wilt of cauliflower caused by V. longisporum. Single and combined inoculations of VerticilliumVt305 and V. longisporum were performed on cauliflower seedlings. Symptom development was evaluated, and fungal colonization was measured in the roots, hypocotyl and stem with real-time PCR. No symptoms were observed after single inoculation of VerticilliumVt305, although it colonized the plant tissues. Pre-inoculation of VerticilliumVt305 reduced symptom development and colonization of plant tissues by V. longisporum. VerticilliumVt305 is an endophyte on cauliflower plants and showed effective biological control of V. longisporum in controlled conditions. This work can contribute to the development of a sustainable control measure of V. longisporum in Brassicaceae hosts, which is currently not available. Additionally, this study provides evidence for the different roles of Verticillium species present in the agro-ecosystem. © 2014 The Society for Applied Microbiology.

Detecting Pathogens of Verticillium Wilt in Winter Oilseed Rape Using ELISA and PCR – Comparison of the Two Methods and With Visual Stand Evaluation

Directory of Open Access Journals (Sweden)

Tomáš Spitzer

2017-01-01

Full Text Available During 2013–2015, oilseed rape samples from various locations in the Czech Republic were analysed for the presence of Verticillium wilt. Samples were evaluated in the lab using ELISA and PCR as well as by visual evaluation during the samples collection. A comparison of detection match in individual methods also was made. ELISA and PCR matched in detecting Verticillium wilt in 60 % of cases. For practical use, a higher match rate would be necessary in cases where samples were to be analysed in various laboratories using only one of these techniques. The possibility was demonstrated to use kits as well as primers not targeted specifically to just Verticillium longisporum but recording both main species on oilseed rape (V. longisporum and V. dahliae. The match rates of lab analyses with visual evaluation of stand infection were surprisingly high at 62 % for ELISA and 77 % for PCR. All three stand evaluation methods matched in 56 % of cases.

The Verticillium-specific protein VdSCP7 localizes to the plant nucleus and modulates immunity to fungal infections.

Science.gov (United States)

Zhang, Lisha; Ni, Hao; Du, Xuan; Wang, Sheng; Ma, Xiao-Wei; Nürnberger, Thorsten; Guo, Hui-Shan; Hua, Chenlei

2017-07-01

Fungal pathogens secrete effector proteins to suppress plant basal defense for successful colonization. Resistant plants, however, can recognize effectors by cognate R proteins to induce effector-triggered immunity (ETI). By analyzing secretomes of the vascular fungal pathogen Verticillium dahliae, we identified a novel secreted protein VdSCP7 that targets the plant nucleus. The green fluorescent protein (GFP)-tagged VdSCP7 gene with either a mutated nuclear localization signal motif or with additional nuclear export signal was transiently expressed in Nicotiana benthamiana, and investigated for induction of plant immunity. The role of VdSCP7 in V. dahliae pathogenicity was characterized by gene knockout and complementation, and GFP labeling. Expression of the VdSCP7 gene in N. benthamiana activated both salicylic acid and jasmonate signaling, and altered the plant's susceptibility to the pathogens Botrytis cinerea and Phytophthora capsici. The immune response activated by VdSCP7 was highly dependent on its initial extracellular secretion and subsequent nuclear localization in plants. Knockout of the VdSCP7 gene significantly enhanced V. dahliae aggressiveness on cotton. GFP-labeled VdSCP7 is secreted by V. dahliae and accumulates in the plant nucleus. We conclude that VdSCP7 is a novel effector protein that targets the host nucleus to modulate plant immunity, and suggest that plants can recognize VdSCP7 to activate ETI during fungal infection. © 2017 The Authors. New Phytologist © 2017 New Phytologist Trust.

Recovery of Microsclerotia of Verticilium dahliae from Soil as Subjected to Various Treatments

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Loekas Soesanto

2002-07-01

Full Text Available The influence of various conditions on the survival of microsclerotia of Verticilium dahliae Kleb. was studied using three sources of field-collected potato stems densely covered with naturally-formed microsclerotia. Microsclerotia were found to survive for up 2 years in potato stems not incorporated in soil. The effects of temperature, pF, including weekly variations in temperature and or pF, and various modes of incorporating potato stem tissue on the survival of microsclerotia for up to 1 year in a sandy unsterilised soil was also studied for different microsclerotia sources. Inoculum source had no signicant effect. Remarkably few microslcerotia were recovered one day after the start of experiments varying between 5.5 and 31%. Recovery remained at this level or even decreased for another month and for several treatments, also after 3 and 6 months. Only after 3 to 12 months, recovery increased to values up to 5 times higher than that of one day after start of the experiment, but recoveries did not exceed the number of microsclerotia initially incorporated into the soil. Changes in recovery may be due to variation in the level of soil mycostasis which is affected by rate of nutrient exudation from microsclerotia. Key words: survival, microsclerotia, Verticillium dahliae

Transcriptomic analysis of the Olea europaea L. roots during the Verticillium dahliae early infection process

DEFF Research Database (Denmark)

Jiménez-Ruiz, Jaime; Pérez, Maria De la O Leyva; Schilirò, Elisabetta

2017-01-01

Olive cultivation is affected by a wide range of biotic constraints. Verticillium wilt of olive is one of the most devastating diseases affecting this woody crop, inflicting major economic losses in many areas, particularly within the Mediterranean Basin. Little is known about gene-expression cha...

Distribution of Tomato spotted wilt virus in dahlia plants.

Science.gov (United States)

Asano, S; Hirayama, Y; Matsushita, Y

2017-04-01

Tomato spotted wilt virus (TSWV) causes significant losses in the production of the ornamental plant Dahlia variabilis in Japan. The purpose of this study was to examine the distribution of TSWV in dahlia plants and identify plant parts that can be used in the selection of TSWV-free plants. The distribution of TSWV was investigated using reverse transcriptional polymerase chain reaction (RT-PCR) and tissue blot immunoassay. The detection rate of TSWV in latent infected compound leaves was the highest in the petiole, and it decreased from the veins and rachis to the lamina. The tissue blot immunoassays of the leaflets showed an uneven distribution of TSWV, especially along the edge of the leaf blade. In stems, the detection rate of TSWV was high partway up the stem compared to that in the upper and the lower parts of the stem during the vegetative growth stage. A highly uneven distribution was observed in the bulb. Our results indicated that middle parts of the stem as well as the petioles, rachis, and veins of compound leaves are suitable for detection of TSWV in dahlias. This study is the first to report uneven distribution of TSWV in dahlia plants. In this study, the distribution of Tomato spotted wilt virus (TSWV) in various parts of dahlia plants was investigated for the first time. The distribution of TSWV was uneven in compound leaves, leaflets, stems, and bulbs. The middle parts of the stem or the petiole and leaf veins should be sampled to detect TSWV when selecting healthy plants. © 2017 The Society for Applied Microbiology.

Cepa de Trichoderma útil para el tratamiento y/o prevención de infecciones provocadas por hongos pertenecientes al género Verticillium

OpenAIRE

Barroso-Albarracín, Juan Bautista; Chaki, Mounira; Mercado-Blanco, Jesús; Valverde-Corredor, Antonio; López García, Antonio Alejandro

2011-01-01

La invención se refiere al uso de la cepa de hongos Trichoderma harzianum CECT 2413 para la prevención y/o tratamiento de infecciones de plantas y/o suelos provocadas por hongos fitopatógenos pertenecientes al género Verticíllium, preferiblemente por los patotipos defoliante o no defoliante de Verticillium dahliae, agente causante de la Verticilosis del olivo. Además, la invención propone el uso de esta cepa de hongos en combinación con otros hongos del género Trichoderma.

Detection of Verticillium wilt of olive trees and downy mildew of opium poppy using hyperspectral and thermal UAV imagery

Science.gov (United States)

Calderón Madrid, Rocío; Navas Cortés, Juan Antonio; Montes Borrego, Miguel; Landa del Castillo, Blanca Beatriz; Lucena León, Carlos; Jesús Zarco Tejada, Pablo

2014-05-01

The present study explored the use of high-resolution thermal, multispectral and hyperspectral imagery as indicators of the infections caused by Verticillium wilt (VW) in olive trees and downy mildew (DM) in opium poppy fields. VW, caused by the soil-borne fungus Verticillium dahliae, and DM, caused by the biotrophic obligate oomycete Peronospora arborescens, are the most economically limiting diseases of olive trees and opium poppy, respectively, worldwide. V. dahliae infects the plant by the roots and colonizes its vascular system, blocking water flow and eventually inducing water stress. P. arborescens colonizes the mesophyll, appearing the first symptoms as small chlorotic leaf lesions, which can evolve to curled and thickened tissues and systemic infections that become deformed and necrotic as the disease develops. The work conducted to detect VW and DM infection consisted on the acquisition of time series of airborne thermal, multispectral and hyperspectral imagery using 2-m and 5-m wingspan electric Unmanned Aerial Vehicles (UAVs) in spring and summer of three consecutive years (2009 to 2011) for VW detection and on three dates in spring of 2009 for DM detection. Two 7-ha commercial olive orchards naturally infected with V. dahliae and two opium poppy field plots artificially infected by P. arborescens were flown. Concurrently to the airborne campaigns, olive orchards and opium poppy fields were assessed "in situ" to assess actual VW severity and DM incidence. Furthermore, field measurements were conducted at leaf and crown level. The field results related to VW detection showed a significant increase in crown temperature (Tc) minus air temperature (Ta) and a decrease in leaf stomatal conductance (G) as VW severity increased. This reduction in G was associated with a significant increase in the Photochemical Reflectance Index (PRI570) and a decrease in chlorophyll fluorescence. DM asymptomatic leaves showed significantly higher NDVI and lower green/red index

A cupin domain-containing protein with a quercetinase activity (VdQase regulates Verticillium dahliae’s pathogenicity and contributes to counteracting host defenses

Directory of Open Access Journals (Sweden)

Abdel eElHadrami

2015-06-01

Full Text Available We previously identified rutin as part of potato root responses to its pathogen Verticillium dahliae. Rutin was directly toxic to the pathogen at doses greater than 160 μM, a threshold below which many V. dahliae pathogenicity-related genes were up-regulated. We identified and characterized a cupin domain-containing protein (VdQase with a dioxygenase activity and a potential role in V. dahliae-potato interactions. The pathogenicity of VdQase knock-out mutants generated through Agrobacterium tumefasciens-mediated transformation was significantly reduced on susceptible potato cultivar Kennebec compared to wild type isolates. Fluorescence microscopy revealed a higher accumulation of flavonols in the stems of infected potatoes and a higher concentration of rutin in the leaves in response to the VdQase mutants as compared to wild type isolates. This, along with the HPLC characterization of high residual and non-utilized quercetin in presence of the knockout mutants, indicates the involvement of VdQase in the catabolism of quercetin and possibly other flavonols in planta. Quantification of Salicylic and Jasmonic Acids (SA, JA in response to the mutants versus wild type isolates revealed involvement of VdQase in the interference with signaling, suggesting a role in pathogenicity. It is hypothesized that the by-product of dioxygenation 2-protocatechuoylphloroglucinolcarboxylic acid, after dissociating into phloroglucinol and protocatechuoyl moieties, becomes a starting point for benzoic acid and SA, thereby interfering with the JA pathway and affecting the interaction outcome. These events may be key factors for V. dahliae in countering potato defenses and becoming notorious in the rhizosphere.

Unravelling aspects of spatial and temporal distribution of Verticillium dahliae in olive, maple and ash trees and improvement of detection methods

NARCIS (Netherlands)

Keykhasaber, Mojtaba

2017-01-01

Vascular wilts caused by xylem-colonizing pathogens are among the most devastating plant diseases that affect a wide range of plant species worldwide. Information on the distribution of V. dahliae in infected trees helps to design an appropriate and efficient sampling method for reliable

Arsenal of elevated defense proteins fails to protect tomato against Verticillium dahliae.

Science.gov (United States)

Robb, Jane; Shittu, Hakeem; Soman, Kizhake V; Kurosky, Alexander; Nazar, Ross N

2012-08-01

Although the hypersensitive reaction in foliar plant diseases has been extensively described, little is clear regarding plant defense strategies in vascular wilt diseases affecting numerous economically important crops and trees. We have examined global genetic responses to Verticillium wilt in tomato (Lycopersicon esculentum Mill.) plants differing in Ve1 resistance alleles. Unexpectedly, mRNA analyses in the susceptible plant (Ve1-) based on the microarrays revealed a very heroic but unsuccessful systemic response involving many known plant defense genes. In contrast, the response is surprisingly low in plants expressing the Ve1+ R-gene and successfully resisting the pathogen. Similarly, whole-cell protein analyses, based on 2D gel electrophoresis and mass spectrometry, demonstrate large systemic increases in a variety of known plant defense proteins in the stems of susceptible plants but only modest changes in the resistant plant. Taken together, the results indicate that the large systemic increases in plant defense proteins do not protect the susceptible plant. Indeed, since a number of the highly elevated proteins are known to participate in the plant hypersensitive response as well as natural senescence, the results suggest that some or all of the disease symptoms, including ultimate plant death, actually may be the result of this exaggerated plant response.

Multimodel Inference for the Prediction of Disease Symptoms and Yield Loss of Potato in a Two-Year Crop Rotation Experiment

Directory of Open Access Journals (Sweden)

Wim Van den Berg

2012-01-01

Full Text Available The second order Akaike information criterion was used for the assessment of 139 regression models for three responses of potato test crops: (a incidence of Spongospora subterranea on the harvested tubers, (b percentage of haulms infected with Verticillium dahliae, and (c tuber yield. Six variables that are likely related to the response variables were taken into consideration: soil infestations of the fungus Verticillium dahliae and of three nematode species (Globodera pallida, Trichodoridae, and Meloidogyne spp. and, furthermore, soil pH and water soluble phosphor (P. Interactions between V. dahliae and the three nematode species were included as well. Based on multimodelling, predictors are given a weight from which one may decide about the need to include them in a prediction of crop yield. The most important predictors were soil infestation levels of V. dahliae and G. pallida and soil pH. The outcome also showed that tubers suffered more from S. subterranea for higher soil pH values. Finally, yield reduction from the presence of V. dahliae was enhanced by the presence of higher densities of G. pallida.

Association mapping of resistance to Verticillium wilt in Gossypium ...

African Journals Online (AJOL)

Verticillium wilt is a major disease affecting the growth of cotton. For screening the resistant genes, 320 Gossypium hirsutum germplasms were evaluated in Verticillium nursery, and association mapping was used to detect the markers associated with the Verticillium wilt resistance. 106 microsatellite marker primer pairs ...

Antimicrobial Activity of Extracts from Leaves, Stems and Flowers of Euphorbia macroclada against plant pathogenic fungi

OpenAIRE

K. Al-Mughrabi

2003-01-01

Extracts drawn from dried and powdered flowers, stems and leaves of Euphorbia macroclada with some organic solvents were tested for antimicrobial effect against the fungi Verticillium dahliae, Fusarium oxysporum, Rhizopus stolonifer, Penicillium italicum, Rhizoctonia solani, Alternaria solani, Stemphylium solani, Cladosporium sp., Mucor sp., and Pythium sp. The strongest inhibitory effect of the extracts was observed against R. solani, V. dahliae, F. oxysporum, Pythium sp. and R. ...

Improvement of some vegetatively propagated ornamentals by gamma radiation

International Nuclear Information System (INIS)

Das, P.K.; Dube, S.; Ghosh, P.; Dhua, S.D.

1977-01-01

Studies were made to induce mutations in some vegetatively propagated ornamentals like, Chrysanthemum, Dahlia, Hibiscus, Acalpha, Iresine, etc. by acute as well as chronic gamma irradiation. In both the types of irradiation experiments, apart from growth reduction and various physiological anomalies in growth and leaf characters, a total of 27 somatic mutants have been isolated in Chrysanthemum, Dahlia, Hibiscus, Acalypha and Iresine. Some of the mutants have been established by perpetuation over 3-5 vegetative generations while few could not be maintained on account of initially small chimeric changes of failure of regeneration of the propagable materials. However, most of the mutants hold as practical improvements and will find place in ornamental improvement programme in the country. (author)

Arabidopsis wat1 (walls are thin1)-mediated resistance to the bacterial vascular pathogen, Ralstonia solanacearum, is accompanied by cross-regulation of salicylic acid and tryptophan metabolism

NARCIS (Netherlands)

Denancé, N.; Ranocha, P.; Oria, N.; Barlet, X.; Rivière, M.P.; Yadeta, K.A.; Hoffmann, L.; Perreau, F.; Clément, G.; Maia-Grondard, A.; Berg, van den G.C.M.; Savelli, B.; Fournier, S.; Aubert, Y.; Pelletier, S.; Thomma, B.P.H.J.; Molina, A.; Jouanin, L.; Marco, Y.; Goffner, D.

2013-01-01

Inactivation of Arabidopsis WAT1 (Walls Are Thin1), a gene required for secondary cell-wall deposition, conferred broad-spectrum resistance to vascular pathogens, including the bacteria Ralstonia solanacearum and Xanthomonas campestris pv. campestris, and the fungi Verticillium dahliae and

Biologische grondontsmetting bij de teelt van trekheesters: Implementatie van een alternatieve ontsmettingsmerhode in de praktijk

NARCIS (Netherlands)

Ludeking, D.J.W.; Hamelink, R.; Bloemhard, C.M.J.; Slooten, van M.A.

2011-01-01

This report is about biological soil disinfestation with an organic product named Herbie 7022. The effect has been investigated against Verticillium dahliae, Meloidogyne incognita and larvae of Serica brunnea. All larvae of Serica brunnea are killed after application of a period of anaerobic

Pseudomonas community structure and antagonistic potential in the rhizosphere : insights gained by combining phylogenetic and functional gene-based analyses

NARCIS (Netherlands)

Costa, Rodrigo; Gomes, Newton C. M.; Kroegerrecklenfort, Ellen; Opelt, Katja; Berg, Gabriele; Smalla, Kornelia

The Pseudomonas community structure and antagonistic potential in the rhizospheres of strawberry and oilseed rape (host plants of the fungal phytopathogen Verticillium dahliae) were assessed. The use of a new PCR-DGGE system, designed to target Pseudomonas-specific gacA gene fragments in

Host range of Verticillium isaacii and Verticillium klebahnii from artichoke, spinach, and lettuce

Science.gov (United States)

Verticillium is a genus that includes major vascular wilt pathogens. The recent multilocus phylogenetic analyses of the genus identified 5 new species including V. isaacii and V. klebahnii, both of which occur in agricultural soils in coastal California, and have been isolated from asymptomatic and ...

Comparative Genomics Yields Insights into Niche Adaptation of Plant Vascular Wilt Pathogens

NARCIS (Netherlands)

Klosterman, S.J.; Subbarao, K.V.; Kang, S.; Veronese, P.; Gold, S.E.; Thomma, B.P.H.J.; Chen, Z.J.; Henrissat, B.; Lee, Y.H.; Park, J.; Garcia-Pedrajas, M.D.; Barbara, D.J.; Anchieta, A.; Jonge, de R.; Santhanam, P.; Maruthachalam, K.; Atallah, Z.; Amyotte, S.G.; Paz, Z.; Inderbitzin, P.; Hayes, R.J.; Heiman, D.I.; Young, S.; Zeng, Q.; Engels, R.; Galagan, J.; Cuomo, C.; Dobinson, K.F.; Ma, L.J.

2011-01-01

The vascular wilt fungi Verticillium dahliae and V. albo-atrum infect over 200 plant species, causing billions of dollars in annual crop losses. The characteristic wilt symptoms are a result of colonization and proliferation of the pathogens in the xylem vessels, which undergo fluctuations in

Multimodel Inference for the Prediction of Disease Symptoms and Yield Loss of Potato in a Two-Year Crop Rotation Experiment

NARCIS (Netherlands)

Berg, van den W.; Vos, J.; Grasman, J.

2012-01-01

The second order Akaike information criterion was used for the assessment of 139 regression models for three responses of potato test crops: (a) incidence of Spongospora subterranea on the harvested tubers, (b) percentage of haulms infected with Verticillium dahliae, and (c) tuber yield. Six

Tomato immune receptor Ve1 recognizes effector of multiple fungal pathogens uncovered by genome and RNA sequencing

NARCIS (Netherlands)

Jonge, de R.; Esse, van H.P.; Maruthachalam, K.; Bolton, M.D.; Santhanam, P.; Keykha Saber, M.; Zhang, Z.; Usami, T.; Lievens, B.; Subbarao, K.V.; Thomma, B.

2012-01-01

Fungal plant pathogens secrete effector molecules to establish disease on their hosts, and plants in turn use immune receptors to try to intercept these effectors. The tomato immune receptor Ve1 governs resistance to race 1 strains of the soil-borne vascular wilt fungi Verticillium dahliae and

Isolation and propagation of mutations in dahlia by in vitro culture

International Nuclear Information System (INIS)

Asahira, T.; Yamagata, H.; Inagaki, M.; Osuga, S.

1975-01-01

The present study was undertaken to search for a successful method for in vitro culture of mutated tissues in dahlia. Preceding the objective, the features of induced mutations and the effects of cutting propagation in dahlia were investigated, and the tissues easily regenerating plantlets in vitro were searched following the examination of effective condition of medium. Induction of mutations: Tuberous roots of two cultivars, 'Kosei' and 'Sunlight', were irradiated with 1,000 - 2,000 R of X-rays. Chlorophyll and flower-color mutations were successfully induced in both cultivars, but the frequency differed with genotypic constitution. The maximum frequency was observed at leaves and shoots on or from the fourth to fifth nodes from the base of plant. The use of M 1 tuberous roots seemed a way for isolating mutations though not so much efficient. Tissue culture: In vitro cultured basal parts of ray florets, exactly the ovary, differentiated shoots. No shoot formation occurred in receptacle and leaf cultures, while roots were differentiated in leaf culture. Supplements of auxin and adenine to the medium besides cytokinin appeared to be necessary for inducing shoots. It is a serious problem in the tissue culture of dahlia that a large number of explants are endogenously comtaminated with bacteria. Taking into consideration low rates of surviving and regenerating explants, it seems difficult at present for dahlia to conclude whether or not the tissue culture may become efficient in mutation breeding as compared with cutting propagation. (author)

Erwinia chrysanthemi ook bij ploffers in Dahlia boosdoener

NARCIS (Netherlands)

Leeuwen, van P.J.; Trompert, J.P.T.

2006-01-01

Sinds een aantal jaren komt bij de stekproduktie van Dahlia veel uitval voor door ploffers. Na het verhogen van de kastemperatuur vallen de knollen natrot weg. Bovendien kan verdere besmetting snel om zich heen grijpen. Onderzoek heeft aangetoond dat de bacterie Erwinia chrysanthemi de veroorzaker

Biological control agent against verticillium wilt

CSIR Research Space (South Africa)

Gumede, WHN

2006-04-01

Full Text Available Verticillium wilt Presented at the 14th Biennial congress of the South African Society for Microbiology WHN Gumede CSIR Biosciences 11-04-06 Slide 2 © CSIR 2006 www.csir.co.za INTRODUCTION • Historical background • Application...

Determination of long-term effects of consecutive effective fresh ...

African Journals Online (AJOL)

The aim of the study was to determine the weed density and the most economical way of weed control in eggplant (Solanum melongena) fields contaminated with Verticillium dahliae (Kleb) after the application of fresh chicken manure and solarization in the second year as the same crop was grown. The effect of solarization ...

Voltammogram of stainless steel/Fe-Co-Ni electrode on water electrolysis in base condition with dahlia pinnata tuber starch media

Science.gov (United States)

Isana S. Y., L.; Yuanita, Dewi; Sulistyani, Al, Heru Pratomo

2017-08-01

Hydrogen production in a safe, enviromentally friendly, and inexpensive is an attempt to realize energy needs commercially, one of them is electrolysis. Many attempts which relate with water electrolysis had been conducted to produce hydrogen, for example by using wastewater as water substitution. The research is to study the effect of dahlia pinnata tuber starch to stainless steel/Fe-Co-Ni electrode activity on water electrolysis in base condition. Stainless steel/Fe-Co-Ni electrode activity for breaking the water molecules eventually is better than stainless steel electrode, either there is existance of dahlia pinnata tuber starch or not. The presence of dahlia pinnata tuber starch apparently makes the covering on surface of the electrode so the catalytic activity of the electrode is reduced. Covering is mostly affected by dahlia pinnata tuber starch concentration. Wastewater which contains starch, especially dahlia pinnata tuber starch, obviously is not good enough because hydrogen production rate becomes obstructed.

Identification of molecular markers associated with Verticillium wilt resistance in alfalfa (Medicago sativa L.) using high-resolution melting.

Science.gov (United States)

Zhang, Tiejun; Yu, Long-Xi; McCord, Per; Miller, David; Bhamidimarri, Suresh; Johnson, David; Monteros, Maria J; Ho, Julie; Reisen, Peter; Samac, Deborah A

2014-01-01

Verticillium wilt, caused by the soilborne fungus, Verticillium alfalfae, is one of the most serious diseases of alfalfa (Medicago sativa L.) worldwide. To identify loci associated with resistance to Verticillium wilt, a bulk segregant analysis was conducted in susceptible or resistant pools constructed from 13 synthetic alfalfa populations, followed by association mapping in two F1 populations consisted of 352 individuals. Simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers were used for genotyping. Phenotyping was done by manual inoculation of the pathogen to replicated cloned plants of each individual and disease severity was scored using a standard scale. Marker-trait association was analyzed by TASSEL. Seventeen SNP markers significantly associated with Verticillium wilt resistance were identified and they were located on chromosomes 1, 2, 4, 7 and 8. SNP markers identified on chromosomes 2, 4 and 7 co-locate with regions of Verticillium wilt resistance loci reported in M. truncatula. Additional markers identified on chromosomes 1 and 8 located the regions where no Verticillium resistance locus has been reported. This study highlights the value of SNP genotyping by high resolution melting to identify the disease resistance loci in tetraploid alfalfa. With further validation, the markers identified in this study could be used for improving resistance to Verticillium wilt in alfalfa breeding programs.

Identification of molecular markers associated with Verticillium wilt resistance in alfalfa (Medicago sativa L. using high-resolution melting.

Directory of Open Access Journals (Sweden)

Tiejun Zhang

Full Text Available Verticillium wilt, caused by the soilborne fungus, Verticillium alfalfae, is one of the most serious diseases of alfalfa (Medicago sativa L. worldwide. To identify loci associated with resistance to Verticillium wilt, a bulk segregant analysis was conducted in susceptible or resistant pools constructed from 13 synthetic alfalfa populations, followed by association mapping in two F1 populations consisted of 352 individuals. Simple sequence repeat (SSR and single nucleotide polymorphism (SNP markers were used for genotyping. Phenotyping was done by manual inoculation of the pathogen to replicated cloned plants of each individual and disease severity was scored using a standard scale. Marker-trait association was analyzed by TASSEL. Seventeen SNP markers significantly associated with Verticillium wilt resistance were identified and they were located on chromosomes 1, 2, 4, 7 and 8. SNP markers identified on chromosomes 2, 4 and 7 co-locate with regions of Verticillium wilt resistance loci reported in M. truncatula. Additional markers identified on chromosomes 1 and 8 located the regions where no Verticillium resistance locus has been reported. This study highlights the value of SNP genotyping by high resolution melting to identify the disease resistance loci in tetraploid alfalfa. With further validation, the markers identified in this study could be used for improving resistance to Verticillium wilt in alfalfa breeding programs.

Variety of plant pathogens of ornamental shrubs of the genus Rosa L.

Directory of Open Access Journals (Sweden)

Marchenko Alla Borisovna

2015-12-01

In the Ukraine’s forest-steppe environment, over the years of research, the most common disease among different representatives of the genus Rosa L. is Diplocarpon rosae FA Wolf, about 52.7%. Diseases caused by Sphaerotheca pannosa var. rosae Woron account for 28.3%: Coniothyrium wernsdorffiae Laubert―19.2%; Botrytis cinerea Pers.―16.9%; Phragmidium mucronatum (Pers. Schltdl., Phragmidium tuberculatum Müll. Hal., Nectria cinnabarina (Tode Fr.―from 7.2 to 6.7%. Pathogens Alternaria alternata (Fr. Keissl., Alternaria tenuissima (Kunze Wiltshire, Fusarium oxysporum Schltdl., Fusarium solani (Mart. Sacc., Phytophthora cactorum J. Schröt., Verticillium dahliae Kleb., Verticillium albo-atrum Reinke Berthold are less common―5%.

Horizontal transfer generates genetic variation in an asexual pathogen

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Xiaoqiu Huang

2014-10-01

Full Text Available There are major gaps in the understanding of how genetic variation is generated in the asexual pathogen Verticillium dahliae. On the one hand, V. dahliae is a haploid organism that reproduces clonally. On the other hand, single-nucleotide polymorphisms and chromosomal rearrangements were found between V. dahliae strains. Lineage-specific (LS regions comprising about 5% of the genome are highly variable between V. dahliae strains. Nonetheless, it is unknown whether horizontal gene transfer plays a major role in generating genetic variation in V. dahliae. Here, we analyzed a previously sequenced V. dahliae population of nine strains from various geographical locations and hosts. We found highly homologous elements in LS regions of each strain; LS regions of V. dahliae strain JR2 are much richer in highly homologous elements than the core genome. In addition, we discovered, in LS regions of JR2, several structural forms of nonhomologous recombination, and two or three homologous sequence types of each form, with almost each sequence type present in an LS region of another strain. A large section of one of the forms is known to be horizontally transferred between V. dahliae strains. We unexpectedly found that 350 kilobases of dynamic LS regions were much more conserved than the core genome between V. dahliae and a closely related species (V. albo-atrum, suggesting that these LS regions were horizontally transferred recently. Our results support the view that genetic variation in LS regions is generated by horizontal transfer between strains, and by chromosomal reshuffling reported previously.

Eindrapportage onderzoek naar ontwikkeling Verticillium resistente Acer

NARCIS (Netherlands)

Hiemstra, J.A.; Sluis, van der B.J.

2013-01-01

Dit project is de laatste fase in het onderzoek naar de ontwikkeling van Verticillium-resistente Acer platanoides (Noorse esdoorn) onderstammen wat in 1993 van start ging op het toenmalige CPRO-DLO (nu PRI) met grootschalige selectie-experimenten. PPO-Bomen heeft vanaf 1999 onderzoek gedaan naar

Real-time PCR for the quantification of fungi in planta.

Science.gov (United States)

Klosterman, Steven J

2012-01-01

Methods enabling quantification of fungi in planta can be useful for a variety of applications. In combination with information on plant disease severity, indirect quantification of fungi in planta offers an additional tool in the screening of plants that are resistant to fungal diseases. In this chapter, a method is described for the quantification of DNA from a fungus in plant leaves using real-time PCR (qPCR). Although the method described entails quantification of the fungus Verticillium dahliae in lettuce leaves, the methodology described would be useful for other pathosystems as well. The method utilizes primers that are specific for amplification of a β-tubulin sequence from V. dahliae and a lettuce actin gene sequence as a reference for normalization. This approach enabled quantification of V. dahliae in the amount of 2.5 fg/ng of lettuce leaf DNA at 21 days following plant inoculation.

Usage of the Heterologous Expression of the Antimicrobial Gene afp From Aspergillus giganteus for Increasing Fungal Resistance in Olive

Science.gov (United States)

Narvaez, Isabel; Khayreddine, Titouh; Pliego, Clara; Cerezo, Sergio; Jiménez-Díaz, Rafael M.; Trapero-Casas, José L.; López-Herrera, Carlos; Arjona-Girona, Isabel; Martín, Carmen; Mercado, José A.; Pliego-Alfaro, Fernando

2018-01-01

The antifungal protein (AFP) produced by Aspergillus giganteus, encoded by the afp gene, has been used to confer resistance against a broad range of fungal pathogens in several crops. In this research, transgenic olive plants expressing the afp gene under the control of the constitutive promoter CaMV35S were generated and their disease response against two root infecting fungal pathogens, Verticillium dahliae and Rosellinia necatrix, was evaluated. Embryogenic cultures derived from a mature zygotic embryo of cv. ‘Picual’ were used for A. tumefaciens transformation. Five independent transgenic lines were obtained, showing a variable level of afp expression in leaves and roots. None of these transgenic lines showed enhanced resistance to Verticillium wilt. However, some of the lines displayed a degree of incomplete resistance to white root rot caused by R. necatrix compared with disease reaction of non-transformed plants or transgenic plants expressing only the GUS gene. The level of resistance to this pathogen correlated with that of the afp expression in root and leaves. Our results indicate that the afp gene can be useful for enhanced partial resistance to R. necatrix in olive, but this gene does not protect against V. dahliae. PMID:29875785

Functional Analysis of the Tomato Immune Receptor Ve1 through Domain Swaps with Its Non-Functional Homolog Ve2

Science.gov (United States)

Rovenich, Hanna; Song, Yin; Liebrand, Thomas W. H.; Masini, Laura; van den Berg, Grardy C. M.; Joosten, Matthieu H. A. J.; Thomma, Bart P. H. J.

2014-01-01

Resistance in tomato against race 1 strains of the fungal vascular wilt pathogens Verticillium dahliae and V. albo-atrum is mediated by the Ve locus. This locus comprises two closely linked inversely oriented genes, Ve1 and Ve2, which encode cell surface receptors of the extracellular leucine-rich repeat receptor-like protein (eLRR-RLP) type. While Ve1 mediates Verticillium resistance through monitoring the presence of the recently identified V. dahliae Ave1 effector, no functionality for Ve2 has been demonstrated in tomato. Ve1 and Ve2 contain 37 eLRRs and share 84% amino acid identity, facilitating investigation of Ve protein functionality through domain swapping. In this study it is shown that Ve chimeras in which the first thirty eLRRs of Ve1 were replaced by those of Ve2 remain able to induce HR and activate Verticillium resistance, and that deletion of these thirty eLRRs from Ve1 resulted in loss of functionality. Also the region between eLRR30 and eLRR35 is required for Ve1-mediated resistance, and cannot be replaced by the region between eLRR30 and eLRR35 of Ve2. We furthermore show that the cytoplasmic tail of Ve1 is required for functionality, as truncation of this tail results in loss of functionality. Moreover, the C-terminus of Ve2 fails to activate immune signaling as chimeras containing the C-terminus of Ve2 do not provide Verticillium resistance. Furthermore, Ve1 was found to interact through its C-terminus with the eLRR-containing receptor-like kinase (eLRR-RLK) interactor SOBIR1 that was recently identified as an interactor of eLRR-RLP (immune) receptors. Intriguingly, also Ve2 was found to interact with SOBIR1. PMID:24505431

Complete genome sequence of Bacillus subtilis BSD-2, a microbial germicide isolated from cultivated cotton.

Science.gov (United States)

Liu, Hongwei; Yin, Shuli; An, Likang; Zhang, Genwei; Cheng, Huicai; Xi, Yanhua; Cui, Guanhui; Zhang, Feiyan; Zhang, Liping

2016-07-20

Bacillus subtilis BSD-2, isolated from cotton (Gossypium spp.), had strong antagonistic activity to Verticillium dahlia Kleb and Botrytis cinerea. We sequenced and annotated the BSD-2 complete genome to help us the better use of this strain, which has surfactin, bacilysin, bacillibactin, subtilosin A, Tas A and a potential class IV lanthipeptide biosynthetic pathways. Copyright © 2016 Elsevier B.V. All rights reserved.

Biological control of Rhizoctonia solani on potato by Verticillium ...

African Journals Online (AJOL)

STORAGESEVER

2009-06-03

Jun 3, 2009 ... V. biguttatum isolates affected R. solani by antibiosis and parasitism. All isolates of V. ... After the coiling around of R. solani hyphae, V. ... is an important fungal pathogen ... Gliocladium spp., Trichoderma spp. and Verticillium.

Improving protein mass and cumulative body weight gain of local chicken fed ration fortified with a combination of Lactobacillus sp. and dahlia inulin

Science.gov (United States)

Wahyuni, H. I.; Suthama, N.; Mangisah, I.; Krismiyanto, L.

2018-01-01

The research aimed to evaluate meat calcium and protein content of local chicken fed diet fortified with a combination of Lactobacillus sp and Dahlia Inulin. One hundred and twenty birds of 4 months old local chicken with average body weight of 1001 g were assigned in a completely randomized design with 4 treatments and 5 replications. The treatments were the farmer formulated ration (FF) and the improved ration (IR), fortified with 1.2% inulin and 1.2 ml Lactobacillus sp. (FFIL and IRIL). Parameters were calcium retention, protein coefficient digestibility, meat calcium and protein mass, and cumulative body weight gain. The results showed that all parameters were significantly affected by dietary treatments. The improved ration resulted in higher calcium retention and protein coefficient digestibility than the farmer formulated ration when fed by both with and without fortification of dahlia inulin and Lactobacillus sp. Meat protein mass of chicken fed by both FR and IR fortified with dahlia inulin and Lactobacillus sp. showed higher value than chicken fed by unfortified FR and IR. Cumulative body weight gain of chicken fed by both FR and IR fortified with dahlia inulin and Lactobacillus sp. also showed higher value than chicken fed by without fortification. In conclusion, both FR and IR fortified with dahlia inulin and Lactobacillus sp. improved meat protein mass and cumulative body weight gain, especially the farmer formulated ration was pronouncedly improved by fortification of Lactobacillus sp. and dahlia inulin.

Catalytic Synthesis and Antifungal Activity of New Polychlorinated Natural Terpenes

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Hana Ighachane

2017-01-01

Full Text Available Various unsaturated natural terpenes were selectively converted to the corresponding polychlorinated products in good yields using iron acetylacetonate in combination with nucleophilic cocatalyst. The synthesized compounds were evaluated for their in vitro antifungal activity. The antifungal bioassays showed that 2c and 2d possessed significant antifungal activity against Fusarium oxysporum f. sp. albedinis (Foa, Fusarium oxysporum f. sp. canariensis (Foc, and Verticillium dahliae (Vd.

Performance evaluation of extractor cutting blade configuration in Inulin extraction process from Dahlia sp. L tuber

Science.gov (United States)

Sundari, E.; Praputri, E.; Marthiana, W.; Jaya, M.

2018-03-01

Inulin, a polysaccharide plant-based nutrient, can be isolated from dahlia flower tubers by liquid-solid extraction processes and is generally carried out in an extractor tank equipped with an agitator. To accelerate the diffusion rate of solute from the solid phase (bulk phase) to the external surface (boundary layer) in order to increase yield of inulin, the size reduction of material is required. The purpose of this research was to design the cutting blade needed for dahlia tuber size reduction and investigate the effect of blade types, agitator speed (350, 700, 1050, and 1400 rpm), and configuration of cutting blade to material fineness at 90 minutes of contacting time. The results showed that higher cutting blade speed results in higher cut material fineness rate. The best conditions was achieved by the configuration of two four-blade turbine combined with one three-blade turbine with fineness rate more than 90% in 30 minutes of contacting time at every variation of agitator speed. The cutting blade designed in this study can be used for size reduction purpose of tubers other than dahlia tubers.

Lactic acid fermentation of dahlia tuber starch and waste using Lactobacillus bulgaricus: A comparative study

Science.gov (United States)

Praputri, E.; Sundari, E.; Martynis, M.; Agenta, P.

2018-03-01

Lactic acid fermentation of dahlia tuber starch and waste was performed by means of Lactobacillus bulgaricus through enzymatic hydrolysis followed by fermentation process. The effect of pH condition on lactic acid production was investigated during the process. The selected bacteria produced lactic acid after 24 hours of fermentation and the productivity was increase after 24 hours of fermentation. After 120 hours of fermentation, it was found that dahlia tuber starch can produce up to 16.18% of lactic acid, whereas lactic acid produced from dahlia tuber waste was only 0.40% at pH of 4. The lactic acid production increase significantly for pH 3.5 and 4 until 96 hours of fermentation, then slowed down. On the other hand, for pH 4.5 the lactic acid production increase until 48 hours of fermentation and then slowed down. The identification of fermentation product indicated that the lactic acid produced in this study was 16.20%, acidic, yellow and cloudy with pH 3.4 – 4.2. The density of lactic acid produced ranged between 1.21 to 1.25 gr/ml.

Induction of somatic mutations in some vegetatively Propagated ornamentals by gamma radiation

International Nuclear Information System (INIS)

Das, P.K.; Ghosh, P.; Dube, S.; Dhua, S.P.

1974-01-01

Attempts were made to produce mutations in some vegetatively propagated ornamentals by acute and chronic gamma irradiation. Rooted cutting of Chrysanthemums and dormant bulbs of Dahlias were exposed to 1.5, 2.5, 3.5 and 2.0, 3.0 kR of acute doses respectively. In chronic irradiation, potted plants of Bougainvillea, Hibiscus, Allamanda, Achania, Jasminum, Chrysanthemum were exposed to several dose rates at different isodose arcs in the gamma field. Isolated mutants are being vegetatively multiplied and some of them have already been stabilized as pure forms. (M.G.B.)

Methylation-sensitive amplified polymorphism analysis of Verticillium wilt-stressed cotton (Gossypium).

Science.gov (United States)

Wang, W; Zhang, M; Chen, H D; Cai, X X; Xu, M L; Lei, K Y; Niu, J H; Deng, L; Liu, J; Ge, Z J; Yu, S X; Wang, B H

2016-10-06

In this study, a methylation-sensitive amplification polymorphism analysis system was used to analyze DNA methylation level in three cotton accessions. Two disease-sensitive near-isogenic lines, PD94042 and IL41, and one disease-resistant Gossypium mustelinum accession were exposed to Verticillium wilt, to investigate molecular disease resistance mechanisms in cotton. We observed multiple different DNA methylation types across the three accessions following Verticillium wilt exposure. These included hypomethylation, hypermethylation, and other patterns. In general, the global DNA methylation level was significantly increased in the disease-resistant accession G. mustelinum following disease exposure. In contrast, there was no significant difference in the disease-sensitive accession PD94042, and a significant decrease was observed in IL41. Our results suggest that disease-resistant cotton might employ a mechanism to increase methylation level in response to disease stress. The differing methylation patterns, together with the increase in global DNA methylation level, might play important roles in tolerance to Verticillium wilt in cotton. Through cloning and analysis of differently methylated DNA sequences, we were also able to identify several genes that may contribute to disease resistance in cotton. Our results revealed the effect of DNA methylation on cotton disease resistance, and also identified genes that played important roles, which may shed light on the future cotton disease-resistant molecular breeding.

Antimicrobial Activity of Extracts from Leaves, Stems and Flowers of Euphorbia macroclada against plant pathogenic fungi

Directory of Open Access Journals (Sweden)

K. Al-Mughrabi

2003-12-01

Full Text Available Extracts drawn from dried and powdered flowers, stems and leaves of Euphorbia macroclada with some organic solvents were tested for antimicrobial effect against the fungi Verticillium dahliae, Fusarium oxysporum, Rhizopus stolonifer, Penicillium italicum, Rhizoctonia solani, Alternaria solani, Stemphylium solani, Cladosporium sp., Mucor sp., and Pythium sp. The strongest inhibitory effect of the extracts was observed against R. solani, V. dahliae, F. oxysporum, Pythium sp. and R. stolonifer. The weakest effect was against A. solani. Extracts from the stems had a stronger inhibitory effect than those from the flowers or leaves. Butanol was the best solvent to extract antimicrobial compounds from leaves, stems and flowers and was superior to chloroform, water and petroleum ether. Results clearly indicate that E. macroclada is a promising source of antimicrobial compounds.

Preventing an increase in Verticillium wilt incidence in spinach seed production

DEFF Research Database (Denmark)

Olesen, Merete Halkjær; Deleuran, Lise Christina; Gislum, René

2014-01-01

A semifield assay was conducted from 2009 to 2011 to distinguish between different preventive methods of reducing Verticillium spp. in spinach seed production. The seed treatments for controlling seed infection levels included Thiram, Signum, Trichoderma harzianum, Gliocladium roseum and Natural II...

PHYTOPATHOLOGICAL ASSESMENT OF CAPSICUM ANNUUM L. (SWEET PEPPER AND CHILLI GERMPLASM FROM THE GENE POOL OF THE WORLD VEGETABLE CENTER (AVRDC UNDER THE CONDITIONS OF ARARAT VALLEY IN ARMENIA

Directory of Open Access Journals (Sweden)

K. M. Sarikyan

2015-01-01

Full Text Available On an experimental basis of the Scientific Center of vegetable, melon and industrial crops (Republic of Armenia 20 accessions of the world germplasm of Capsicum annuum L. (sweet pepper, chili provided by the World Vegetable Center (AVRDC have been studied. The accessions resistant to such diseases as verticillium wilt (Verticillium albo-atrum Reincke, bacterial wilt (Ralstonia solanacearum (= Pseudomonas solanacearum, tobacco mosaic virus (TMV and cucumber mosaic virus (CMV, blossom end rot of fruit, and tip-burn have been selected. These genotypes of chili and sweet pepper are the promising initial material in breeding for resistance to common diseases. In collaboration with other breeders, five varieties of peppers were developed and released: sweet pepper cultivars Natalie, Emily, Miles, and chili cultivars Zita and Gita.

FEEDING EFFECT OF INULIN DERIVED FROM DAHLIA TUBER COMBINED WITH Lactobacillus sp. ON MEAT PROTEIN MASS OF CROSSBRED KAMPONG CHICKEN

Directory of Open Access Journals (Sweden)

Z. H. Abdurrahman

2016-03-01

Full Text Available The objective of the study was to determine the effects of feeding Lactobacillus species (Lactobacillus sp. and inulin derived from dahlia tuber powder on antioxidant activity, calcium mass, and protein mass of crossbred kampong chicken meat. A total of  168 birds of 21 days old crossbred kampong chickens were randomly allocated into 6 treatments with four replications per treatment. The present experiment was assigned in  a completely randomized design with 2 x 3 factorial scheme. The first factor was levels of dahlia tuber powder, namely 0.8% (A1 and 1.2% (A2, and the second factor was levels of Lactobacillus sp., namely none (B0, 1.2 mL (108 cfu/mL/B1 and 2.4 mL (108 cfu/mL/B2. The parameters measured were antioxidant activity, meat calcium and protein mass. Data were subjected to analysis of variance and followed by Duncan multiple range test (P<0.05 when the treatment indicated significant effect. The supplementation of dahlia tuber powder and Lactobacillus sp. significantly (P<0.05 increased antioxidant activity and protein mass of meat. However, calcium mass of meat was not significantly affected by treatments. In conclusion, feeding dahlia tuber powder at the level of 1.2% combined with Lactobacillus sp. at 1.2 mL (108 cfu/mL, can be categorized as the best combination based on the increase in antioxidant activity and meat protein mass.  

FERMENTATION OF INULIN BY A NEW STRAIN OF CLOSTRIDIUM-THERMOAUTOTROPHICUM ISOLATED FROM DAHLIA TUBERS

NARCIS (Netherlands)

DRENT, WJ; GOTTSCHAL, JC

1991-01-01

A new inulin-fermenting strain of Clostridium thermoautotrophicum was isolated through enrichment on dahlia tubers, and subsequent plating on agar media with undissolved inulin. Both the cell-bound and cell-free inulinase(s) functioned optimally at 60-degrees-C and at neutral pH. This new strain I1

Molecular and physiological diversity among Verticillium fungicola var. fungicola and var. aleophilum

NARCIS (Netherlands)

Largeteau, M.L.; Baars, J.J.P.; Savoie, J.M.

2006-01-01

The genetic and physiological variability of Verticillium fungicola var. aleophilum responsible for Agaricus bisporus dry bubble disease in North America is well documented but little is known about the var. fungicola affecting European crops. Variability was assessed within this variety and

Mutagenesis and breeding for disease resistance in capsicum

International Nuclear Information System (INIS)

Saccardo, F.; Sree Ramulu, K.

1977-01-01

The principal diseases, for which no sources have so far been found within the cultivars of Capsicum annuum in Italy, are caused by Verticillium dahliae, Phytophthora capsici and cucumber mosaic virus (CMV). The wild species C. pendulum, C. frutescens, C. chinense, C. chacoense, C. pubescens and C. eximium were analysed to find out if the sources for resistance to the three diseases are available. It was observed that particularly the species C. frutescens and C. chinense had good sources of resistance to V. dahliae and Ph. capsici. However, the occurrence of reproductive barriers between the wild and cultivated species appears to be a problem for the transfer of disease-resistant genes. For CMV, none of the wild species showed good resistance; so in this case a screening technique was set up using mutagenic agents to isolate resistant types in the prominent agronomic cultivars of C. annuum. Also, for V. dahliae and Ph. capsici, mutation screening techniques were set up to induce disease resistance character directly in the cultivars of C. annuum, without causing any changes in the most important agronomic characters of the cultivars. (author)

Optimization of Verticillium lecanii spore production in solid-state fermentation on sugarcane bagasse

NARCIS (Netherlands)

Shi, Y.; Xu, X.; Zhu, Y.

2009-01-01

Verticillium lecanii is an entomopathogen with high potential in biological control of pests. We developed a solid-state fermentation with sugarcane bagasse as carrier absorbing liquid medium to propagate V. lecanii spores. Using statistical experimental design, we optimized the medium composition

Plant host range of Verticillium longisporum and microsclerotia density in Swedisch soils

NARCIS (Netherlands)

Johansson, A.; Goud, J.C.; Dixelius, C.

2006-01-01

Verticillium longisporum is a soil-borne fungal pathogen causing vascular wilt of Brassica crops. This study was conducted to enhance our knowledge on the host range of V. longisporum. Seven crop species (barley, oat, oilseed rape, pea, red clover, sugar beet and wheat) and five weed species (barren

Draft Genome Sequence and Gene Annotation of the Entomopathogenic Fungus Verticillium hemipterigenum

OpenAIRE

Horn, Fabian; Habel, Andreas; Scharf, Daniel H.; Dworschak, Jan; Brakhage, Axel A.; Guthke, Reinhard; Hertweck, Christian; Linde, J?rg

2015-01-01

Verticillium hemipterigenum (anamorph Torrubiella hemipterigena) is an entomopathogenic fungus and produces a broad range of secondary metabolites. Here, we present the draft genome sequence of the fungus, including gene structure and functional annotation. Genes were predicted incorporating RNA-Seq data and functionally annotated to provide the basis for further genome studies.

Functional characterization of the recombinant antimicrobial peptide Trx-Ace-AMP1 and its application on the control of tomato early blight disease.

Science.gov (United States)

Wu, Yin; He, Yue; Ge, Xiaochun

2011-05-01

Ace-AMP1 is a potent antifungal peptide found in onion (Allium cepa) seeds with sequence similarity to plant lipid transfer proteins. Transgenic plants over-expressing Ace-AMP1 gene have enhanced disease resistance to some fungal pathogens. However, mass production in heterologous systems and in vitro application of this peptide have not been reported. In this study, Ace-AMP1 was highly expressed in a prokaryotic Escherichia coli system as a fusion protein. The purified protein inhibited the growth of many plant fungal pathogens, especially Alternaria solani, Fusarium oxysporum f. sp. vasinfectum, and Verticillium dahliae. The inhibitory effect was accompanied by hyphal hyperbranching for V. dahliae but not for F. oxysporum f. sp. vasinfectum and A. solani, suggesting that the mode of action of Ace-AMP1 on different fungi might be different. Application of Ace-AMP1 on tomato leaves showed that the recombinant protein conferred strong resistance to the tomato pathogen A. solani and could be used as an effective fungicide.

Fungal Competitors Affect Production of Antimicrobial Lipopeptides in Bacillus subtilis Strain B9-5.

Science.gov (United States)

DeFilippi, Stefanie; Groulx, Emma; Megalla, Merna; Mohamed, Rowida; Avis, Tyler J

2018-04-01

Bacillus subtilis has shown success in antagonizing plant pathogens where strains of the bacterium produce antimicrobial cyclic lipopeptides (CLPs) in response to microbial competitors in their ecological niche. To gain insight into the inhibitory role of these CLPs, B. subtilis strain B9-5 was co-cultured with three pathogenic fungi. Inhibition of mycelial growth and spore germination was assessed and CLPs produced by B. subtilis B9-5 were quantified over the entire period of microbial interaction. B. subtilis B9-5 significantly inhibited mycelial growth and spore germination of Fusarium sambucinum and Verticillium dahliae, but not Rhizopus stolonifer. LC-MS analysis revealed that B. subtilis differentially produced fengycin and surfactin homologs depending on the competitor. CLP quantification suggested that the presence of Verticillium dahliae, a fungus highly sensitive to the compounds, caused an increase followed by a decrease in CLP production by the bacterium. In co-cultures with Fusarium sambucinum, a moderately sensitive fungus, CLP production increased more gradually, possibly because of its slower rate of spore germination. With co-cultures of the tolerant fungus Rhizopus stolonifer, B. subtilis produced high amounts of CLPs (per bacterial cell) for the duration of the interaction. Variations in CLP production could be explained, in part, by the pathogens' overall sensitivities to the bacterial lipopeptides and/or the relative growth rates between the plant pathogen and B. subtilis. CLP production varied substantially temporally depending on the targeted fungus, which provides valuable insight concerning the effectiveness of B. subtilis B9-5 protecting its ecological niche against the ingress of these pathogens.

In vitro inhibition of pathogenic Verticillium dahliae, causal agent of ...

African Journals Online (AJOL)

SAM

2014-08-13

Aug 13, 2014 ... In addition, plant pathogens directly affected through antibiosis and ... Trichoderma strains for antagonistic activity on the fungal pathogen V. ... Five soil sub samples were taken from the area around the healthy potato roots ...

Meat Characteristic of Crossbred Local Chicken Fed Inulin of Dahlia Tuber and Lactobacillus sp.

Directory of Open Access Journals (Sweden)

Zakaria Husein Abdurrahman

2016-08-01

Full Text Available This study was conducted to evaluate the meat characteristic of crossbred local chicken fed diet containing both dahlia tuber powder as inulin source and probiotic Lactobacillus sp. The experimental animals were 168 crossbred local chickens which were randomly divided into 6 groups of treatment (4 replications each when they were 21-d old. A completely randomized design with 2 x 3 factorial pattern consisted of 2 levels of prebiotic [(0.8% (D1 and 1.2% (D2] and 3 levels of probiotic [without probiotic (L0, 1.2 mL (L1, and 2.4 mL (L2] was arranged in the present study. One mL probiotic (Lactobacillus sp. was equal to 108 cfu. Results showed that the supplementation of prebiotic and probiotic significantly (P<0.05 affected breast meat color in terms of L* (lightness and b* (yellowness. The meat fat mass and cholesterol was significantly (P<0.05 decreased by the combination of prebiotic and probiotic. The hardness of meat was not affected significantly by all treatments. The conclusion is that breast meat color could be improved, and both meat fat mass and cholesterol content could be decreased by feeding a combination of 1.2% dahlia tuber powder as inulin source and 1.2 mL probiotic Lactobacillus sp.

Host respostas transcricionais para vasculares e foliar fungos fitopatogênicos

OpenAIRE

Almeida, Robson Ferreira de

2009-01-01

O presente trabalho estudou mecanismos genéticos que controlam a resposta de plantas à uma espécie fitopatogênica do gênero Verticillium. V. dahliae (Vd) e V. longisoporum (Vl) são fungos de solo causadores de doenças vasculares de plantas herbáceas e plantas arbóreas, estas duas espécies são responsáveis por perdas anuais que somam bilhões de dólares. Este patógneo perde um alto grau de sua especificidade quanto ao hospedeiro e viabiliza a colonização e multiplicação em diferentes espécies ...

Cotton plants export microRNAs to inhibit virulence gene expression in a fungal pathogen.

Science.gov (United States)

Zhang, Tao; Zhao, Yun-Long; Zhao, Jian-Hua; Wang, Sheng; Jin, Yun; Chen, Zhong-Qi; Fang, Yuan-Yuan; Hua, Chen-Lei; Ding, Shou-Wei; Guo, Hui-Shan

2016-09-26

Plant pathogenic fungi represent the largest group of disease-causing agents on crop plants, and are a constant and major threat to agriculture worldwide. Recent studies have shown that engineered production of RNA interference (RNAi)-inducing dsRNA in host plants can trigger specific fungal gene silencing and confer resistance to fungal pathogens 1-7 . Although these findings illustrate efficient uptake of host RNAi triggers by pathogenic fungi, it is unknown whether or not such an uptake mechanism has been evolved for a natural biological function in fungus-host interactions. Here, we show that in response to infection with Verticillium dahliae (a vascular fungal pathogen responsible for devastating wilt diseases in many crops) cotton plants increase production of microRNA 166 (miR166) and miR159 and export both to the fungal hyphae for specific silencing. We found that two V. dahliae genes encoding a Ca 2+ -dependent cysteine protease (Clp-1) and an isotrichodermin C-15 hydroxylase (HiC-15), and targeted by miR166 and miR159, respectively, are both essential for fungal virulence. Notably, V. dahliae strains expressing either Clp-1 or HiC-15 rendered resistant to the respective miRNA exhibited drastically enhanced virulence in cotton plants. Together, our findings identify a novel defence strategy of host plants by exporting specific miRNAs to induce cross-kingdom gene silencing in pathogenic fungi and confer disease resistance.

Data integration and knowledge management to facilitate research on plant-pathogen interactions: case study Solanum tuberosum – Verticillium

Science.gov (United States)

Verticillium wilt is an important disease causing considerable economic losses in potato production globally. Soil and environmental conditions affect symptom expression and the effect of the disease on yield. A ten year field trial has been conducted in order to better understand the dynamics of wi...

Paecilomyces lilacinus and Verticillium chlamydosporium Fungi as Biological Control of Fasciolosis

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Riza Zainuddin Ahmad

2013-09-01

Full Text Available Fasciolosis is a worm disease caused of Fasciola gigantica and an important problem in husbandry especially for cattle. Controlling of this worm disease can be conducted by prevention and treatment. The use of antihelminthic is commonly causes a resistance problem. Natural control by mold such as Paecilomyces lilacinus and Verticillium chlamydosporium can be applied to reduce egg of F. gigantica. Although it was recently found, in vitro study gave satisfied result. This gives a new hope in controlling the disease although the extend application still needs to be studied. This paper discussed about the use of P. lilacinus and V. chlamydosporium for reducing F. gigantica population.

The Emerging British Verticillium longisporum Population Consists of Aggressive Brassica Pathogens.

Science.gov (United States)

Depotter, Jasper R L; Rodriguez-Moreno, Luis; Thomma, Bart P H J; Wood, Thomas A

2017-11-01

Verticillium longisporum is an economically important fungal pathogen of brassicaceous crops that originated from at least three hybridization events between different Verticillium spp., leading to the hybrid lineages A1/D1, A1/D2, and A1/D3. Isolates of lineage A1/D1 generally cause stem striping on oilseed rape (Brassica napus), which has recently been reported for the first time to occur in the United Kingdom. Intriguingly, the emerging U.K. population is distinct from the north-central European stem striping population. Little is known about the pathogenicity of the newly emerged U.K. population; hence, pathogenicity tests were executed to compare British isolates to previously characterized reference strains. In addition to the model plant Arabidopsis thaliana, the pathogenicity of four British isolates was assessed on four cultivars of three Brassica crop species: oilseed rape (Quartz and Incentive), cauliflower (Clapton), and Chinese cabbage (Hilton). To this end, vascular discoloration of the roots, plant biomass accumulations, and fungal stem colonization upon isolate infection were evaluated. The British isolates appeared to be remarkably aggressive, because plant biomass was significantly affected and severe vascular discoloration was observed. The British isolates were successful stem colonizers and the extent of fungal colonization negatively correlated with plant biomass of cauliflower and Quartz oilseed rape. However, in Quartz, the fungal colonization of A1/D1 isolates was significantly lower than that of the virulent reference isolate from lineage A1/D3, PD589. Moreover, despite levels of stem colonization similar to those of A1/D1 strains, PD589 did not cause significant disease on Incentive. Thus, A1/D1 isolates, including British isolates, are aggressive oilseed rape pathogens despite limited colonization levels in comparison with a virulent A1/D3 isolate.

Expression of chloroperoxidase from Pseudomonas pyrrocinia in tobacco plastids for fungal resistance.

Science.gov (United States)

Ruhlman, Tracey A; Rajasekaran, Kanniah; Cary, Jeffrey W

2014-11-01

The chloroperoxidase (cpo) gene from Pseudomonas pyrrocinia was transformed into the plastid genome (plastome) of Nicotiana tabacum var. Petit Havana and transplastomic lines were compared with a nuclear transformant for the same gene. Southern analysis confirmed integration in the plastome and western blotting confirmed the presence of the chloroperoxidase protein (CPO) in higher abundance in transplastomic plants than in cpo nuclear transformants. Northern analysis of primary plastome transformants for cpo showed 15-fold higher transcript abundance than in the nuclear transformant, yet this extent of enhancement was not observed in western blot, enzyme or bioassay, indicating a bottleneck at the post-transcriptional level. Representative plants from the two transplastomic lines showed resistance to fungal pathogens in vitro (Aspergillus flavus, Fusarium verticillioides, and Verticillium dahliae) and in planta (Alternaria alternata). Published by Elsevier Ireland Ltd.

Potential antagonism of some Trichoderma strains isolated from Moroccan soil against three phytopathogenic fungi of great economic importance

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Wafaa MOKHTARI

2017-09-01

Full Text Available In this study, 17 Trichoderma strains were isolated from different soils (crop fields and Argan forests in Morocco. Purified monospore cultures were identified using molecular methods and tested for their potential antagonism against three phytopathogenic fungi (Fusarium oxyxporum, verticillium dahlia and rhizoctonia solani. After DNA extraction, translation elongation factor (tef1 was amplified in extracts of 17 strains, sequenced and compared with their ex-types. As a result, three species were identified among the strains, which clustered in two different subclades of Trichoderma: the species T. afroharzianum, and T. guizhouense belong to the Harzianum clade, while T. longibrachiatum belongs to the Longibrachiatum clade. Investigation of potential antagonistic effects of these strains against the soil-borne phytopathogens F. oxysporum, R. solani and V. dahliae was conducted in a dual culture plate assay, using 17 promising Trichoderma strains that have been selected based on a polymerase chain reaction (PCR screening approach. In vitro, Trichoderma isolates showed effective antagonistic performance by decreasing soil borne pathogens mycelium radial growth. Trichoderma afroharzianum showed the highest Percentage of Radial Inhibition Growth (PRIG %. The highest PRIG% = 98% was for 8A2.3 isolate against R. solani and the lowest PRIG%= 67% for T9i10 against F. oxysporum. On the other hand, T9i12, which is T. reesei species, led to a high radial inhibition of pathogens’ mycelium.

Isolation of Corynebacterium Xerosis from Jordanian Soil and a Study on its Antimicrobial Activity against a Range of Bacteria and Fungi

International Nuclear Information System (INIS)

El-Banna, Nasser

2004-01-01

A bacterial strain which has been identified as Corneybacterium Xerosis NB-2 was isolated from a soil sample from Jerash Private University, Jerash, Jordan. This isolate was found to produce an antimicrobial substance active only against filamentous fungi and yeasts (Aspergillus niger SQ 40, Fusarium oxysporium SQ11, Verticillium dahliae SQ 42, Saccharomyces SQ 46 and Candida albicans SQ 47). However, all tested gram-positive bacteria and gram negative bacteria (Bacillus megaterium SQ5, Bacillus cereus SQ6, Staphylococcus aureus SQ9, Streptococcus pyogens SQ10, Eschericshia coli SQ 22, Klepsiella spp SQ33 and SQ33 and Pseudonomas mallei SQ 34) were found to be resistant. In batch culture, the isolated NB-2 produced the antimicrobial substance late in the growth phase and antimicrobial activity of Corynebacterium Xerosis against filamentous fungi and yeasts which was not previously described. (author)

Endophytic colonization and biocontrol performance of Pseudomonas fluorescens PICF7 in olive (Olea europaea L.) are determined neither by pyoverdine production nor swimming motility.

Science.gov (United States)

Maldonado-González, M Mercedes; Schilirò, Elisabetta; Prieto, Pilar; Mercado-Blanco, Jesús

2015-09-01

Pseudomonas fluorescens PICF7 is an indigenous inhabitant of olive (Olea europaea L.) rhizosphere, able to display endophytic lifestyle in roots, to induce a wide range of defence responses upon colonization of this organ and to exert effective biological control against Verticillium wilt of olive (VWO) (Verticillium dahliae). We aimed to evaluate the involvement of specific PICF7 phenotypes in olive root colonization and VWO biocontrol effectiveness by generating mutants impaired in swimming motility (fliI) or siderophore pyoverdine production (pvdI). Besides, the performance of mutants with diminished in vitro growth in potato dextrose agar medium (gltA) and cysteine (Cys) auxotrophy was also assessed. Results showed that olive root colonization and VWO biocontrol ability of the fliI, pvdI and gltA mutants did not significantly differ from that displayed by the parental strain PICF7. Consequently, altered in vitro growth, swimming motility and pyoverdine production contribute neither to PICF7 VWO suppressive effect nor to its colonization ability. In contrast, the Cys auxotroph mutant showed reduced olive root colonization capacity and lost full biocontrol efficacy. Moreover, confocal laser scanning microscopy revealed that all mutants tested were able to endophytically colonize root tissue to the same extent as wild-type PICF7, discarding these traits as relevant for its endophytic lifestyle. © 2014 Society for Applied Microbiology and John Wiley & Sons Ltd.

Isolation and characterization of a chitinase gene from entomopathogenic fungus Verticillium lecanii Isolamento e caracterização de um gene de quitinase do fungo entomopatogênico Verticillium lecanii

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Yanping Zhu

2008-06-01

Full Text Available Entomopathogenic fungus Verticillium lecanii is a promising whitefly and aphid control agent. Chitinases secreted by this insect pathogen have considerable importance in the biological control of some insect pests. An endochitinase gene Vlchit1 from the fungus was cloned and overexpressed in Escherichia coli. The Vlchit1 gene not only contains an open reading frame (ORF which encodes a protein of 423 amino acids (aa, but also is interrupted by three short introns. A homology modelling of Vlchit1 protein showed that the chitinase Vlchit1 has a (α/β8 TIM barrel structure. Overexpression test and Enzymatic activity assay indicated that the Vlchit1 is a functional enzyme that can hydrolyze the chitin substrate, so the Vlchit1 gene can service as a useful gene source for genetic manipulation leading to strain improvement of entomopathogenic fungi or constructing new transgenic plants with resistance to various fungal and insects pests.O fungo entomopatogênico Verticillium lecanii é um agente promissor no controle da mosca-branca e do pulgão. As quitinases secretadas por esse patógeno de insetos têm uma grande importância no controle biológico de doenças causadas por insetos. Um gene de endoquitinase Vlchit1 desse fungo foi clonado e expresso em Escherichia coli. O gene Vlchit contém não apenas um ORF que codifica uma proteína de 423 aminoácidos, mas também é interrompido por três pequenos introns. A modelagem de homologia da proteína Vlchit1indicou que a quitinase Vlchit1 tem uma estrutura (α/β 8 TIM barrel. Testes de expressão e de atividade enzimática indicaram que Vlchit1 é uma enzima funcional que hidroliza quitina, portanto o gene Vlchit pode ser um gene útil para manipulação genética para melhoramento de cepas de fungos entomopatogênicos ou para a construção de novas plantas transgênicas com resistência a várias doenças causadas por fungos e insetos.

The endochitinase VDECH from Verticillium dahliae inhibits spore germination and activates plant defense responses

Science.gov (United States)

Chitinases function in the digestion of chitin molecules, which are present principally in insects and fungi. In plants, chitinase genes play important roles in defense, and their expression can be triggered in response to both biotic and abiotic stresses. In this study, we cloned and characterized ...

Biochemical indices of hop resistance to Verticillium albo-atrum and Fusarium sambucinum

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Jan Piotrowski

2013-12-01

Full Text Available The contents of total phenolic compounds, chlorogenic acid and peroxidase activity as well as monophenols to polyphenols ratio were studies in the suckers of the hops as indices of resistance to Verticillium albo-atrum and Fusarium sambucinum. The suckers of hop taken in the early spring from the healthy and infected plots were used in the experiments. As a research material were included cv. 'Northern Brewer' - a wilt tolerant variety, two wild susceptible varieties - cv. 'Lubelski' and cv. 'Brewers Gold', four breeding clones and one male plant. It was found that, 'Northern Brewer' contains more total phenolic compounds, rnonophenols and chlorogenic acid, and in particular considerably higher peroxidase activity as compared to cv. 'Lubelski'. Taking into consideration the contents of these compounds, in the majority of cases, the new breeding clones were similar to the mother variety 'Northern Brewer'. It seems resonable to assume, that the new clones should be more wilt tolerant than varieties and populations cultivated in our country.

COMPLEX OF PATHOGENES ON VEGETABLE CROPS IN CONDITION OF CENTRAL REGION OF RUSSIA

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L. T. Timina

2015-01-01

Full Text Available As a result of monitoring of causative agents of diseases of vegetable crops and studying of its species specification, the genus and species of fungi and bacteria, were found. Previously unknown in the Central region of Russia pathogens of carrot were identified: Sclerotinia nevales, Gleocladium roseum, Verticillium spp, Trichotecium roseum, Streptomyces scabies, F. nivale, F. chlamidosporum, F. equiseti, F. proliferatum, Chaetomium spp., Erysiphe umbelliferum, Erwinia carotovora. Main causative agents of diseases  of carrot during storage were also described: Alternaria infectoria, A. alternatа, A. arborescens, A. radicina, A. cheiranthi, A. corotiincultae, A. cinerariae, Embellisia spp., Nimbia spp., Cladosporium spp. It was found new pathogen for onion (Aspergillus niger, garlic (Fusarium semitectum, F. subglutinans, F. proliferatum, F.avenacium, red beet (Typhula ishikariensis, and radish (Drechslera Bondartseva.

Gene cloning: exploring cotton functional genomics and genetic improvement

Institute of Scientific and Technical Information of China (English)

Diqiu LIU; Xianlong ZHANG

2008-01-01

Cotton is the most important natural fiber plant in the world. The genetic improvement of the quality of the cotton fiber and agricultural productivity is imperative under the situation of increasing consumption and rapid development of textile technology. Recently, the study of cotton molecular biology has progressed greatly. A lot of specifically or preferentially expressed cotton fiber genes were cloned and analyzed. On the other hand, identification of stress response genes expressed in cotton was performed by other research groups. The major stress factors were studied including the wilt pathogens Verticillium dahliae, Fusarium oxy-sporum f. sp. vasinfectum, bacterial blight, root-knot nematode, drought, and salt stress. What is more, a few genes related to the biosynthesis of gossypol, other sesquiterpene phytoalexins and the major seed oil fatty acids were isolated from cotton. In the present review, we focused on the major advances in cotton gene cloning and expression profiling in the recent years.

The Vascular Pathogen Verticillium longisporum Does Not Affect Water Relations and Plant Responses to Drought Stress of Its Host, Brassica napus.

Science.gov (United States)

Lopisso, Daniel Teshome; Knüfer, Jessica; Koopmann, Birger; von Tiedemann, Andreas

2017-04-01

Verticillium longisporum is a host-specific vascular pathogen of oilseed rape (Brassica napus L.) that causes economic crop losses by impairing plant growth and inducing premature senescence. This study investigates whether plant damage through Verticillium stem striping is due to impaired plant water relations, whether V. longisporum affects responses of a susceptible B. napus variety to drought stress, and whether drought stress, in turn, affects plant responses to V. longisporum. Two-factorial experiments on a susceptible cultivar of B. napus infected or noninfected with V. longisporum and exposed to three watering levels (30, 60, and 100% field capacity) revealed that drought stress and V. longisporum impaired plant growth by entirely different mechanisms. Although both stresses similarly affected plant growth parameters (plant height, hypocotyl diameter, and shoot and root dry matter), infection of B. napus with V. longisporum did not affect any drought-related physiological or molecular genetic plant parameters, including transpiration rate, stomatal conductance, photosynthesis rate, water use efficiency, relative leaf water content, leaf proline content, or the expression of drought-responsive genes. Thus, this study provides comprehensive physiological and molecular genetic evidence explaining the lack of wilt symptoms in B. napus infected with V. longisporum. Likewise, drought tolerance of B. napus was unaffected by V. longisporum, as was the level of disease by drought conditions, thus excluding a concerted action of both stresses in the field. Although it is evident that drought and vascular infection with V. longisporum impair plant growth by different mechanisms, it remains to be determined by which other factors V. longisporum causes crop loss.

Response of Verticillium fungicola var. fungicola, Mycogone perniciosa_and Cladobotrym sp. Mushroom Pathogens to Some Essential Oils

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Brankica Tanović

2006-01-01

Full Text Available Antifungal activity of 18 essential oils was evaluated against Verticillium fungicola var. fungicola, Mycogone perniciosa and Cladobotryum sp., the causal agents of button mushroom diseases. Essential oils including: turpentine, basil, lemon, mint, fenchel, rose geranium, anise, cinnamon, scots pine, clove, thyme, juniper, lavender, orange, eucalyptus, rosemary, bergamot orange and tea tree, were screened for their effectiveness against the pathogens in vitro. In order to investigate fungicidal activity, isolates were exposed to the volatile phase of the oils for seven days. Of the 18 essential oils analyzed, cinnamon, clove, thyme, and tea tree showed the highest antifungal activity against all investigated mycopathogens,with Minimum Fungicidal Concentration (MFC being 0.02 μl/ml of air. Turpentine essential oil expressed the lowest antifungal effect to all isolates tested.

Entomopathogenic fungi associated with Ixodes ricinus ticks

DEFF Research Database (Denmark)

Kalsbeek, Vibeke; Frandsen, F.; Steenberg, Tove

1995-01-01

to the Hyphomycetes. Paecilomyces farinosus and Verticillium lecanii were the predominant species. Other species, found only on engorged females were: Beauveria bassiana, B. brongniartii, P. fumosoroseus and V. araneanrm. Eight out of 1833 ticks collected from the vegetation and three out of 269 engorged nymphs were...

Extracellular Alkalinization as a Defense Response in Potato Cells.

Science.gov (United States)

Moroz, Natalia; Fritch, Karen R; Marcec, Matthew J; Tripathi, Diwaker; Smertenko, Andrei; Tanaka, Kiwamu

2017-01-01

A quantitative and robust bioassay to assess plant defense response is important for studies of disease resistance and also for the early identification of disease during pre- or non-symptomatic phases. An increase in extracellular pH is known to be an early defense response in plants. In this study, we demonstrate extracellular alkalinization as a defense response in potatoes. Using potato suspension cell cultures, we observed an alkalinization response against various pathogen- and plant-derived elicitors in a dose- and time-dependent manner. We also assessed the defense response against a variety of potato pathogens, such as protists ( Phytophthora infestans and Spongospora subterranea ) and fungi ( Verticillium dahliae and Colletotrichum coccodes ). Our results show that extracellular pH increases within 30 min in proportion to the number of pathogen spores added. Consistently with the alkalinization effect, the higher transcription level of several defense-related genes and production of reactive oxygen species was observed. Our results demonstrate that the alkalinization response is an effective marker to study early stages of defense response in potatoes.

Isolation and characterization of putative endophytic bacteria antagonistic to Phoma tracheiphila and Verticillium albo-atrum.

Science.gov (United States)

Kalai-Grami, Leila; Saidi, Sabrine; Bachkouel, Sarra; Ben Slimene, Imen; Mnari-Hattab, Monia; Hajlaoui, Mohamed Rebah; Limam, Ferid

2014-09-01

A collection of 200 bacterial isolates recovered from citrus plants (Citrus limon, Citrus sinensis, and Citrus reticulata), Medicago truncatula and Laurus nobilis, was established. In vitro screening indicated that 28 isolates exhibited an inhibitory activity against the vascular pathogens Phoma tracheiphila and Verticillium albo-atrum. Isolates were screened according to their hydrolytic activities, plant growth-promoting bacteria (PGPB) abilities, as well as for the presence of nonribosomal peptide synthetase (NRPS) genes responsible of the lipopeptide biosynthesis. The results were positive for 16 isolates which exhibited at least two PGPB activities and a single NRPS gene. Genetic diversity of the selected isolates was studied using random amplified polymorphic DNA (RAPD) and repetitive element PCR (REP) tools that showed clustering of strains into three major groups (I, II, and III) (i, ii, and iii), respectively. Clustering was further confirmed by the 16S rDNA sequencing that assigned nine isolates to Bacillus velezensis, four isolates to Bacillus methyltrophicus, one isolate to Bacillus amyloliquefaciens, and two isolates to Bacillus mojavensis. Organ-bacterial genotype interaction as well as positive correlation with NRPS genes are discussed.

In vitro effect of essential oils from aromatic and medicinal plants on mushroom pathogens: Verticillium fungicola var. fungicola, Mycogone perniciosa, and Cladobotryum sp.

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Tanović Brankica

2009-01-01

Full Text Available Lavender, anise, chamomile, fennel, geranium, oregano, parsley, and sage essential oils were tested for their effectiveness against mushroom pathogens: Verticillium fungicola var. fungicola, Mycogone perniciosa, and Cladobotryum sp. Isolates were exposed to the volatile phase of the oils and then ventilated in order to determine if the effect of the oil was lethal to the pathogen. Oregano and geranium oils were the most toxic, having a fungicidal effect at 0.02-0.08 μl/ml of air, depending on the pathogen. Oregano oil was characterized by high content of carvacrol and thymol, while citranelol and geraniol were the main components of geranium oil.

Growth of Verticillium longisporum in Xylem Sap of Brassica napus is Independent from Cultivar Resistance but Promoted by Plant Aging.

Science.gov (United States)

Lopisso, Daniel Teshome; Knüfer, Jessica; Koopmann, Birger; von Tiedemann, Andreas

2017-09-01

As Verticillium stem striping of oilseed rape (OSR), a vascular disease caused by Verticillium longisporum, is extending into new geographic regions and no control with fungicides exists, the demand for understanding mechanisms of quantitative resistance increases. Because V. longisporum is strictly limited to the xylem and resistance is expressed in the systemic stage post root invasion, we investigated a potential antifungal role of soluble constituents and nutritional conditions in xylem sap as determinants of cultivar resistance of OSR to V. longisporum. Assessment of biometric and molecular genetic parameters applied to describe V. longisporum resistance (net area under disease progress curve, stunting, stem thickness, plant biomass, and V. longisporum DNA content) showed consistent susceptibility of cultivar 'Falcon' in contrast to two resistant genotypes, 'SEM' and 'Aviso'. Spectrophotometric analysis revealed a consistently stronger in vitro growth of V. longisporum in xylem sap extracted from OSR compared with the water control. Further comparisons of fungal growth in xylem sap of different cultivars revealed the absence of constitutive or V. longisporum induced antifungal activity in the xylem sap of resistant versus susceptible genotypes. The similar growth of V. longisporum in xylem sap, irrespective of cultivar, infection with V. longisporum and xylem sap filtration, was correlated with about equal amounts of total soluble proteins in xylem sap from these treatments. Interestingly, compared with younger plants, xylem sap from older plants induced significantly stronger fungal growth. Growth enhancement of V. longisporum in xylem sap of aging plants was reflected by increased contents of carbohydrates, which was consistent in mock or V. longisporum-infected plants and independent from cultivar resistance. The improved nutritional conditions in the xylem of more mature plants may explain the late appearance of disease symptoms, which are observed only in

Purification and characterization of a CkTLP protein from Cynanchum komarovii seeds that confers antifungal activity.

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Qinghua Wang

Full Text Available BACKGROUND: Cynanchum komarovii Al Iljinski is a desert plant that has been used as analgesic, anthelminthic and antidiarrheal, but also as a herbal medicine to treat cholecystitis in people. We have found that the protein extractions from C. komarovii seeds have strong antifungal activity. There is strong interest to develop protein medication and antifungal pesticides from C. komarovii for pharmacological or other uses. METHODOLOGY/PRINCIPAL FINDINGS: An antifungal protein with sequence homology to thaumatin-like proteins (TLPs was isolated from C. komarovii seeds and named CkTLP. The three-dimensional structure prediction of CkTLP indicated the protein has an acid cleft and a hydrophobic patch. The protein showed antifungal activity against fungal growth of Verticillium dahliae, Fusarium oxysporum, Rhizoctonia solani, Botrytis cinerea and Valsa mali. The full-length cDNA was cloned by RT-PCR and RACE-PCR according to the partial protein sequences obtained by nanoESI-MS/MS. The real-time PCR showed the transcription level of CkTLP had a significant increase under the stress of abscisic acid (ABA, salicylic acid (SA, methyl jasmonate (MeJA, NaCl and drought, which indicates that CkTLP may play an important role in response to abiotic stresses. Histochemical staining showed GUS activity in almost the whole plant, especially in cotyledons, trichomes and vascular tissues of primary root and inflorescences. The CkTLP protein was located in the extracellular space/cell wall by CkTLP::GFP fusion protein in transgenic Arabidopsis. Furthermore, over-expression of CkTLP significantly enhanced the resistance of Arabidopsis against V. dahliae. CONCLUSIONS/SIGNIFICANCE: The results suggest that the CkTLP is a good candidate protein or gene for contributing to the development of disease-resistant crops.

Genome-wide identification of mitogen-activated protein kinase gene family in Gossypium raimondii and the function of their corresponding orthologs in tetraploid cultivated cotton.

Science.gov (United States)

Zhang, Xueying; Wang, Liman; Xu, Xiaoyang; Cai, Caiping; Guo, Wangzhen

2014-12-10

Mitogen-activated protein kinase (MAPK) cascades play a crucial role in plant growth and development as well as biotic and abiotic stress responses. Knowledge about the MAPK gene family in cotton is limited, and systematic investigation of MAPK family proteins has not been reported. By performing a bioinformatics homology search, we identified 28 putative MAPK genes in the Gossypium raimondii genome. These MAPK members were anchored onto 11 chromosomes in G. raimondii, with uneven distribution. Phylogenetic analysis showed that the MAPK candidates could be classified into the four known A, B, C and D groups, with more MAPKs containing the TEY phosphorylation site (18 members) than the TDY motif (10 members). Furthermore, 21 cDNA sequences of MAPKs with complete open reading frames (ORFs) were identified in G. hirsutum via PCR-based approaches, including 13 novel MAPKs and eight with homologs reported previously in tetraploid cotton. The expression patterns of 23 MAPK genes reveal their important roles in diverse functions in cotton, in both various developmental stages of vegetative and reproductive growth and in the stress response. Using a reverse genetics approach based on tobacco rattle virus-induced gene silencing (TRV-VIGS), we further verified that MPK9, MPK13 and MPK25 confer resistance to defoliating isolates of Verticillium dahliae in cotton. Silencing of MPK9, MPK13 and MPK25 can significantly enhance cotton susceptibility to this pathogen. This study presents a comprehensive identification of 28 mitogen-activated protein kinase genes in G. raimondii. Their phylogenetic relationships, transcript expression patterns and responses to various stressors were verified. This study provides the first systematic analysis of MAPKs in cotton, improving our understanding of defense responses in general and laying the foundation for future crop improvement using MAPKs.

Diversity and Antimicrobial Properties of Lactic Acid Bacteria Isolated from Rhizosphere of Olive Trees and Desert Truffles of Tunisia

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Imene Fhoula

2013-01-01

Full Text Available A total of 119 lactic acid bacteria (LAB were isolated, by culture-dependant method, from rhizosphere samples of olive trees and desert truffles and evaluated for different biotechnological properties. Using the variability of the intergenic spacer 16S-23S and 16S rRNA gene sequences, the isolates were identified as the genera Lactococcus, Pediococcus, Lactobacillus, Weissella, and Enterococcus. All the strains showed proteolytic activity with variable rates 42% were EPS producers, while only 10% showed the ability to grow in 9% NaCl. In addition, a low rate of antibiotic resistance was detected among rhizospheric enterococci. Furthermore, a strong antibacterial activity against plant and/or pathogenic bacteria of Stenotrophomonas maltophilia, Pantoea agglomerans, Pseudomonas savastanoi, the food-borne Staphylococcus aureus, and Listeria monocytogenes was recorded. Antifungal activity evaluation showed that Botrytis cinerea was the most inhibited fungus followed by Penicillium expansum, Verticillium dahliae, and Aspergillus niger. Most of the active strains belonged to the genera Enterococcus and Weissella. This study led to suggest that environmental-derived LAB strains could be selected for technological application to control pathogenic bacteria and to protect food safety from postharvest deleterious microbiota.

Induced mutation of new cotton lines tolerant to verticillium wilt with improved characters

International Nuclear Information System (INIS)

Rastegary, G.; Hoseiny Neghad, Z.

1998-01-01

Induction of mutation for genetic variation has been used in crop improvement for many years. The mutant lines can be used either directly or as a new genetic source in cross breeding. In cotton 'eleven' and 'two' mutant varieties as new genetic sources have been evolved directly and indirectly, respectively. One of the major obstacles in cotton production in northern region of Iran, Gorgan and Gonbad (where they are known as the main cultivation area of this crop), is the presence of verticillium wilt fungal disease. Since this fungus is soil-born, and can not be controlled chemically, the most efficient way of combating against the disease is to breed for the tolerance/resistance of the species. For this purpose, a mutation breeding technique was applied using gamma radiation as mutagen. The seeds of four varieties (Shirpan, Tashkand, Bakhtegan, and Sahel) were irradiated after reaching a proper absorbed humidity. The radiation doses of 150 to 350 Gy were applied and the seeds were cultivated in two different locations (Varamin and Kordkuy) as M1 generation. The cotton balls of each individual healthy plant was harvested to attain the seeds of M2 rows. In M2, the plants with different degrees of tolerance to the disease were compared to the selected parents (taking into consideration that the soil was contaminated). The good yielding lines with different level of tolerance were taken up to the 5th generation, yielding 70 lines of superior qualitative and quantitative traits. (author)

The role of carotenoids in protection of verticillium agaricinum against lethal effects of gamma radiation

International Nuclear Information System (INIS)

Osman, M.; Adam, Y.M.; Elwy, E.E.A.; Attaby, H.S.H.

1991-01-01

Gamma irradiation markedly inhibited growth activities of non-pigmented and pigmented cultures of verticillium agaricinum. however, the survival of the spores from the latter was higher than that of the former group especially in the case of high doses. The spores of both types of cultures failed to develop mycelial growth on solid and liquid media especially after irradiation with 1000 krad. Some of the spores form pigmented cultures treated with gamma irradiation at level 800 krad were able to survive and produce growth on solid and liquid media while the same treatment of non-pigmented cultures resulted in very weak growth on liquid medium only. Gamma irradiation caused an increase in DNA content of both types of cultures, while RNA decreased in cultures developed from non-pigmented cultures treated with gamma rays of 400 krad. In cultures originated from pigmented cultures and treated with gamma irradiation of different doses, RNA content showed no significant difference in the dose range from 100-400 krad. On the other hand, 800 krad increased significantly RNA content. Total protein of cultures developed from pigmented ones and irradiated with various doses of gamma rays was more or less the same as the controls, while in the case of non-pigmented cultures protein content increased significantly when irradiated with 800 krad

The Fusarium oxysporum effector Six6 contributes to virulence and suppresses I-2-mediated cell death.

Science.gov (United States)

Gawehns, F; Houterman, P M; Ichou, F Ait; Michielse, C B; Hijdra, M; Cornelissen, B J C; Rep, M; Takken, F L W

2014-04-01

Plant pathogens secrete effectors to manipulate their host and facilitate colonization. Fusarium oxysporum f. sp. lycopersici is the causal agent of Fusarium wilt disease in tomato. Upon infection, F. oxysporum f. sp. lycopersici secretes numerous small proteins into the xylem sap (Six proteins). Most Six proteins are unique to F. oxysporum, but Six6 is an exception; a homolog is also present in two Colletotrichum spp. SIX6 expression was found to require living host cells and a knockout of SIX6 in F. oxysporum f. sp. lycopersici compromised virulence, classifying it as a genuine effector. Heterologous expression of SIX6 did not affect growth of Agrobacterium tumefaciens in Nicotiana benthamiana leaves or susceptibility of Arabidopsis thaliana toward Verticillium dahliae, Pseudomonas syringae, or F. oxysporum, suggesting a specific function for F. oxysporum f. sp. lycopersici Six6 in the F. oxysporum f. sp. lycopersici- tomato pathosystem. Remarkably, Six6 was found to specifically suppress I-2-mediated cell death (I2CD) upon transient expression in N. benthamiana, whereas it did not compromise the activity of other cell-death-inducing genes. Still, this I2CD suppressing activity of Six6 does not allow the fungus to overcome I-2 resistance in tomato, suggesting that I-2-mediated resistance is independent from cell death.

Evaluación del Entomopatogeno Verticillium lecanii (Zimm Viegas En el control de la escama blanda Philephedra tuberculosa Nakahara & Gill en Guanábana (Anona muricata L. Evaluation of the entomopatogenic Verticillium lecanii (Zimm. Viega5 in the control of the soft scale Philephedra tuberculosa Nakahara & Gil! in the guanabana (Anona muricata

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Cavallazi Gina

1998-12-01

Full Text Available Una de las alternativas del control de la escama blanda Philephedra tuberculosa Nakahara & Gillen el cultivo de la Guanábana (Anona muricata L., como parte del manejo integrado, la constituye el uso del hongo entomopatógeno Verticillium lecanii (Zimm. Viegas, el cual se consideracomo controlador natural. El presente estudio se realizó con el fin de evaluar la eficacia del entomopatógeno Verticillium lecanii, como agente controlador de esta escama. La evaluación de la eficacia del hongo entomopatógeno, aislado a partir de material coleccionado en campo,se realizó mediante ensayos bajo condiciones de laboratorio y de campo. La producción masiva del hongo estuvo a cargo de una casa especializada y productora,además del establecimiento de dosis para recomendar. La dosisde 1,8 x 107conidiaspor mililitro de suspensión resultó ser la más eficaz en el control de la escama. El análisisde los resultados permitió, de igual forma, determinar que los individuos pertenecientes al segundo  instar ninfal presentan la mayor susceptibilidad al ataque del hongo.One the alternatives in the control of the soft scale Phílephedra tuberculosa Nakahara & Gill in the crop of Guanabana (Anona murícata. within the integral management, is the enthomopathogenic fungus Vertícillium lecaníí (Zimm. Viegas, as a natural control of this scale. This study had as objective, to evaluate the efficacy of Vertícíllíum lecanií as control of this scale. The evaluation of the efficacy of the enthomopatogenic fungus, isolated from material colected in the crop, was done by means assays in laboratory and crop conditions. The masive production of the entomopatogenic fungus was done by a producer, moreover the establishment of a recomended dose (1,8 x 107 conidias/ml that resulted be the most effective in the control of the scale. The analysis of the results permited to determine that the individuals in the second nymphal instar, present most susceptibility to the

Bacterial diversity on the surface of potato tubers in soil and the influence of the plant genotype.

Science.gov (United States)

Weinert, Nicole; Meincke, Remo; Gottwald, Christine; Heuer, Holger; Schloter, Michael; Berg, Gabriele; Smalla, Kornelia

2010-10-01

The surface of tubers might be a reservoir for bacteria that are disseminated with seed potatoes or that affect postharvest damage. The numbers of culturable bacteria and their antagonistic potential, as well as bacterial community fingerprints were analysed from tubers of seven field-grown potato genotypes, including two lines with tuber-accumulated zeaxanthin. The plant genotype significantly affected the number of culturable bacteria only at one field site. Zeaxanthin had no effect on the bacterial plate counts. In dual culture, 72 of 700 bacterial isolates inhibited at least one of the potato pathogens Rhizoctonia solani, Verticillium dahliae or Phytophthora infestans, 12 of them suppressing all three. Most of these antagonists were identified as Bacillus or Streptomyces. From tubers of two plant genotypes, including one zeaxanthin line, higher numbers of antagonists were isolated. Most antagonists showed glucanase, cellulase and protease activity, which could represent mechanisms for pathogen suppression. PCR-DGGE fingerprints of the 16S rRNA genes of bacterial communities from the tuber surfaces revealed that the potato genotype significantly affected the Pseudomonas community structure at one site. However, the genotypes showed nearly identical fingerprints for Bacteria, Actinobacteria, Alphaproteobacteria, Betaproteobacteria, Bacillus and Streptomycetaceae. In conclusion, tuber-associated bacteria were only weakly affected by the plant genotype. © 2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.

Molecular cloning and characterisation of a pathogenesis-related protein CsPR10 from Crocus sativus.

Science.gov (United States)

Gómez-Gómez, L; Rubio-Moraga, A; Ahrazem, O

2011-03-01

Plants have developed many mechanisms to protect themselves against most potential microbial pathogens and diseases. Among these mechanisms, pathogenesis-related proteins are produced as part of the active defence to prevent attack. In this study, a full-length cDNA encoding the CsPR10 protein was identified in fresh saffron stigmas (Crocus sativus). The deduced amino acid sequence from the nucleotide sequence of the coding region showed homology with PR10 proteins. The clone expressed as a protein in fusion with a GST tag produced a 47-kDa protein in E. coli. CsPR10 had ribonuclease activity, with features common to class II-type ribonucleases; its specific activity was quantified as 68.8 U·mg(-1) protein, thus falling within the range of most PR10 proteins exhibiting RNase activity. Antifungal activity of CsPR10 was assayed against Verticillium dahliae, Penicillium sp. and Fusarium oxysporum. CsPR10 inhibited only F. oxysporum growth, and antifungal potency was reflected in a IC(50) of 8.3 μm. Expression analysis showed the presence of high transcript levels in anther and tepal tissues, low levels in stigmas and roots, and no signal detected in leaves. This protein seems to be involved in the active defence response through activation of the jasmonic acid pathway. © 2010 German Botanical Society and The Royal Botanical Society of the Netherlands.

Metabolic patterns of bacterial communities in aerobic compost teas associated with potential biocontrol of soilborne plant diseases

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Catello PANE

2014-09-01

Full Text Available Aerated compost teas (ACTs are organic products obtained by forced aeration of composts suspended in liquid phase. These products may be biological control tools alternative to synthetic fungicides, because ACTs contain antagonistic microorganisms. In this study, soilborne disease suppressive ability of seven water ACTs, extracted from five horticultural residue-based composts, from an animal waste anaerobic solid digestate and from a commercial municipal waste compost, was assessed using in vitro and in vivo systems. All the ACTs inhibited in vitro growth of Verticillium dahliae, Fusarium oxysporum f. sp. lycopersici, Rhizoctonia solani, Sclerotinia minor, Sclerotium rolfsii and Botrytis cinerea. Filter or thermal sterilization eliminated in vitro suppression, suggesting that microorganisms play key roles in pathogen inhibition. Drenching applications of raw ACTs have potential to reduced disease symptoms caused by R. solani on savoy cabbage, S. minor on lettuce and S. rolfsii on pepper, improved the biomass production and did not show any sign of phytotoxicity. Both in vitro and in vivo suppressiveness of ACTs may be explained by antagonistic  bacterial communities that provide general suppression activities. The metabolic BIOLOG GN and GP profiles reflected the functional potential of the numerically dominant members of the microbial communities used as inoculum. This study has demonstrated that useful resident microorganisms, including mainly Gram-positive and Gram-negative antagonistic bacteria, are likely to be responsible for biological control activity of ACTs.

Induced resistance by cresotic acid (3-hydroxy-4-methyl methylbenzoic acid) against wilt disease of melon and cotton

International Nuclear Information System (INIS)

Dong, H.; Li, Z.; Zhang, D.; Li, W.; Tang, W.

2004-01-01

Cresotic acid (3-hydroxy-4-methylbenzoic acid) was proved be active in controlling wilt diseases of melon and cotton plants grown in the house. Soil drench with 200-1000 ppm cresotic acid induced 62-77 %, 69-79 % and 50-60 % protection against Fusarium oxysporum f.sp melonis (FOM) in melon, Fusarium oxysporum f.sp vasinfectum (FOV) and Verticillium dahliae in cotton, respectively. Since no inhibitory effect of cresotic acid on mycelial growth of these three fungual pathogens was observed in vitro, it is suggested that control of these wilt diseases with cresotic acid resulted from induced resistance. Cresotic acid induced resistance in melon plants not only against race 0, race 1, race 2 and race 1,2, but also against a mixture of these four races of FOM, suggesting a non-race- specific resistance. Level of induced resistance by cresotic acid against FOM depended on inoculum pressure applied to melon plants. At 25 day after inoculation with FOM, percentage protection induced by cresotic acid under low inoculum pressure retained a level of 51 %, while under high inoculum pressure percentage protection decreased to only 10 %. High concentrations of cresotic acid significantly reduced plant growth. Reduction in fresh weight of melon (36-51%) and cotton (42-71%) was obtained with 500-1000 ppm cresotic acid, while only less than 8% reduction occurred with 100-200 ppm. (author)

Produção in vitro de enzimas extracelulares por fungos e sua relação com os sintomas descritos em planta hospedeira

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Alice Elias

2015-12-01

Full Text Available RESUMOOs fungos fitopatogênicos habitantes de solo causam perdas econômicas em muitas culturas e são difíceis de serem controlados. Esses fungos podem ser agrupados pelos sintomas comuns que causam nas plantas, bem como pelas enzimas extracelulares que podem produzir. O objetivo do trabalho foi verificar a produção in vitro de enzimas extracelulares por fungos de solo e tentar relacionar essas enzimas com os sintomas que cada fungo causa em planta hospedeira. O ensaio foi delineado em esquema inteiramente casualizado, com dois fatores, sete fungos (Cylindrocladium spathiphylli, Rhizoctonia solani, Sclerotium rolfsii, Ceratocystis fimbriata, Sclerotinia sclerotiorum, Fusarium oxysporum f. sp. dianthi e Verticillium dahliae mais testemunha e seis enzimas (amilase, carboximetilcelulase, lipase, lacase, catalase e gelatinase com 10 repetições. Catalase e gelatinase foram mensuradas por escala de notas, enquanto que as demais pelo cálculo da área da coroa circular. O ensaio foi repetido e a análise foi realizada com os dados de dois ensaios. Os fungos que causam podridão na raiz ou no colo da planta apresentaram maior produção de lacase, enquanto os que causam obstrução, fendas ou até a destruição do sistema vascular demonstraram a prevalência da lipase.

The rhizospheres of traditional medicinal plants in Panxi, China, host a diverse selection of actinobacteria with antimicrobial properties.

Science.gov (United States)

Zhao, Ke; Penttinen, Petri; Chen, Qiang; Guan, Tongwei; Lindström, Kristina; Ao, Xiaoling; Zhang, Lili; Zhang, Xiaoping

2012-06-01

Actinobacteria are a prolific source of antibiotics. Since the rate of discovery of novel antibiotics is decreasing, actinobacteria from unique environments need to be explored. In particular, actinobacterial biocontrol strains from medicinal plants need to be studied as they can be a source of potent antibiotics. We combined culture-dependent and culture-independent methods in analyzing the actinobacterial diversity in the rhizosphere of seven traditional medicinal plant species from Panxi, China, and assessed the antimicrobial activity of the isolates. Each of the plant species hosted a unique set of actinobacterial strains. Out of the 64 morphologically distinct isolates, half were Streptomyces sp., eight were Micromonospora sp., and the rest were members of 18 actinobacterial genera. In particular, Ainsliaea henryi Diels. hosted a diverse selection of actinobacteria, although the 16S ribosomal RNA (rRNA) sequence identity ranges of the isolates and of the 16S rRNA gene clone library were not congruent. In the clone library, 40% of the sequences were related to uncultured actinobacteria, emphasizing the need to develop isolation methods to assess the full potential of the actinobacteria. All Streptomyces isolates showed antimicrobial activity. While the antimicrobial activities of the rare actinobacteria were limited, the growth of Escherichia coli, Verticillium dahliae, and Fusarium oxysporum were inhibited only by rare actinobacteria, and strains related to Saccharopolyspora shandongensis and Streptosporangium roseum showed broad antimicrobial activity.

Biological control of wilt disease complex on tomato crop caused by Meloidogyne javanica and Fusarium oxysporum f.sp. lycopersici by Verticillium leptobactrum.

Science.gov (United States)

Hajji-Hedfi, Lobna; Regaieg, Hajer; Larayedh, Asma; Chihani, Noura; Horrigue-Raouani, Najet

2017-09-23

The efficacy of Verticillium leptobactrum isolate (HR1) was evaluated in the control of root-knot nematode and Fusarium wilt fungus under laboratory and greenhouse conditions. Five concentrations of V. leptobactrum (HR1) isolate were tested for their nematicidal and fungicidal activities against Meloidogyne javanica and Fusarium oxysporum f.sp. lycopersici in vitro. Laboratory trials showed that mycelium growth inhibition of Fusarium wilt fungus was correlated to the increase of the concentration of culture filtrate. All dilutions showed efficiency in reducing the growth of Fusarium oxysporum f.sp. lycopersici. The greatest nematicidal activity was observed at 50, 75, and 100% filtrate dilutions. The egg hatching percentage reached 42%, and the juvenile's corrected mortality registered 90% for the above treatments. In greenhouse experiment, the biocontrol agent fungus enhanced significantly tomato growth components (height and weight of plant and root). The multiplication rate of root-knot nematode and the Fusarium wilt disease incidence declined significantly with soil application of V. leptobactrum as with chemical treatments. The isolate HR1 was efficient to control wilt disease complex caused by M. javanica and Fusarium oxysporum f.sp. lycopersici.

Susceptibility to Verticillium longisporum is linked to monoterpene production by TPS23/27 in Arabidopsis.

Science.gov (United States)

Roos, Jonas; Bejai, Sarosh; Mozūraitis, Raimondas; Dixelius, Christina

2015-02-01

The fungus Verticillium longisporum is a soil-borne plant pathogen of increasing economic importance, and information on plant responses to it is limited. To identify the genes and components involved in the early stages of infection, transcripts in roots of V. longisporum-challenged Arabidopsis Col-0 and the susceptible NON-RACE SPECIFIC DISEASE RESISTANCE 1 (ndr1-1) mutant were compared using ATH1 gene chips. The analysis revealed altered transcript levels of several terpene biosynthesis genes, including the monoterpene synthase TPS23/27. When transgenic 35S:TPS23/27 and TPS23/27-amiRNA plants were monitored the over-expresser line showed enhanced fungal colonization whereas the silenced genotype was indistinguishable from Col-0. Transcript analysis of terpene biosynthesis genes suggested that only the TPS23/27 pathway is affected in the two transgenic genotypes. To confirm changes in monoterpene production, emitted volatiles were determined using solid-phase microextraction and gas chromatography-mass spectrometry. Levels of all identified TPS23/27 monoterpene products were significantly altered in the transgenic plants. A stimulatory effect on conidial germination and hyphal growth of V. longisporum was also seen in co-cultivation with 35S:TPS23/27 plants and upon exposure to 1,8-cineole, the main product of TPS23/27. Methyl jasmonate treatments of myc2-1 and myc2-2 mutants and analysis of TPS23/27:uidA in the myc2-2 background suggested a dependence on jasmonic acid mediated by the transcription factor MYC2. Taken together, our results show that TPS23/27-produced monoterpenes stimulate germination and subsequent invasion of V. longisporum in Arabidopsis roots. © 2014 The Authors The Plant Journal © 2014 John Wiley & Sons Ltd.

Crescimento e esporulação de isolados de Verticillium lecanii sob diferentes condições nutricionais

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Barbosa Claudio Camargo

2002-01-01

Full Text Available O objetivo deste trabalho foi determinar as melhores condições de crescimento e esporulação de dois isolados de Verticillium lecanii (Zimm. Viégas, cultivados em vários meios de cultura, fontes de C, fontes orgânicas e inorgânicas de N e relações C:N. Após 20 dias de cultivo, BDA e Meio Completo mostraram ser os melhores meios de cultura, e a lactose e o amido foram as fontes de C que mais favoreceram o crescimento dos isolados JAB 02 e JAB 45, respectivamente; baixa esporulação foi apresentada por JAB 02, enquanto JAB 45 obteve boa esporulação em glicose e maltose. JAB 02 desenvolveu melhor quando a fonte orgânica de N era casitona; JAB 45 obteve bom desenvolvimento em presença de casitona e peptona, mas houve pouca esporulação em presença de triptona e caseína hidrolisada. As fontes inorgânicas de N que favoreceram o melhor desenvolvimento dos isolados foram (NH42HPO4 e NaNO3, porém, JAB 02 apresentou baixa esporulação. Entre as relações C:N analisadas, JAB 02 obteve melhor crescimento na relação 60:1 e JAB 45 nas relações 5:1, 10:1 e 20:1; baixa esporulação foi obtida por JAB 02 nas relações testadas, mas não se verificou efeito da relação C:N do meio sobre a esporulação dos isolados.

VEGETATION MAPPING IN WETLANDS

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F. PEDROTTI

2004-01-01

Full Text Available The current work examines the main aspects of wetland vegetation mapping, which can be summarized as analysis of the ecological-vegetational (ecotone gradients; vegetation complexes; relationships between vegetation distribution and geomorphology; vegetation of the hydrographic basin lo which the wetland in question belongs; vegetation monitoring with help of four vegetation maps: phytosociological map of the real and potential vegetation, map of vegetation dynamical tendencies, map of vegetation series.

Canopy Modeling of Aquatic Vegetation: Construction of Submerged Vegetation Index

Science.gov (United States)

Ma, Z.; Zhou, G.

2018-04-01

The unique spectral characteristics of submerged vegetation in wetlands determine that the conventional terrestrial vegetation index cannot be directly employed to species identification and parameter inversion of submerged vegetation. Based on the Aquatic Vegetation Radiative Transfer model (AVRT), this paper attempts to construct an index suitable for submerged vegetation, the model simulated data and a scene of Sentinel-2A image in Taihu Lake, China are utilized for assessing the performance of the newly constructed indices and the existent vegetation indices. The results show that the angle index composed by 525 nm, 555 nm and 670 nm can resist the effects of water columns and is more sensitive to vegetation parameters such as LAI. Furthermore, it makes a well discrimination between submerged vegetation and water bodies in the satellite data. We hope that the new index will provide a theoretical basis for future research.

Vegetation dynamics and dynamic vegetation science

NARCIS (Netherlands)

Van der Maarel, E

1996-01-01

his contribution presents a review of the development of the study of vegetation dynamics since 1979, in the framework of a jubilee meeting on progress in the study of vegetation. However, an exhaustive review is both impossible and unnecessary. It is impossible within the few pages available

Vegetation survey: a new focus for Applied Vegetation Science

NARCIS (Netherlands)

Chytry, M.; Schaminee, J.H.J.; Schwabe, A.

2011-01-01

Vegetation survey is an important research agenda in vegetation science. It defines vegetation types and helps understand differences among them, which is essential for both basic ecological research and applications in biodiversity conservation and environmental monitoring. In this editorial, we

Estimation of cancerolytic properties of thionine from plants seeds by inclusion of C14-thymidine in tumoral cells

International Nuclear Information System (INIS)

Pshenichnov, E.A.; Sultanova, E.M.; Kuznetsova, N.N.; Khashimova, Z.S.; Veshkurova, O.N.; Sadikov, A.A.; Salikhov, Sh.I.

2004-01-01

Full text: It has been earlier shown that cysteine rich peptides - thionine from seeds of various plants possess expressed fungitoxic activity. It is connected to influence of thionine on cellular membranes of fungi. It was possible to assume that the substances showing cytotoxic activity will be active in relation to tumoral cells. We isolated peptide fractions from seeds bamia (Hibiscus esculentus), kenaf (Hibiscus cannabinus), abutilon (Abutilon theophrasti), euphorbia (Euphorbia virgata), palma Christi (Ricinus communis) and horse sorrel (Rumex confertus) and studied their antineoplastic and fungitoxic activity. Antiproliferative action of peptides to melanoma cells of mice was estimated in cytotoxic test by inclusion of C 14 -thymidine to DNA. This researches have shown that peptides from seeds of horse sorrel and palma Christi did not change a level of synthesis of DNA while peptides from euphorbia and bamia considerably reduced inclusion of labeled nucleotide to DNA and suppressed growth of tumoral cells on 14 and 39 % accordingly. Parallel tests of these peptides on fungitoxic activity in relation to virulent strains of Verticillium dahliae have shown suppression of conidial growth on 17 and 26 % accordingly. Thus, peptides from seeds of bamia and euphorbia possess the expressed property to suppress growth of tumoral cells and can be used at creation a new cancerolytic preparations for treatment of human cancer. Work is executed under the financial support of fundamental grants F - 4.19 and F-4.1.44

Fumigant dosages below maximum label rate control some soilborne pathogens

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Shachaf Triky-Dotan

2016-08-01

Full Text Available The activity of commercial soil fumigants on some key soilborne pathogens was assessed in sandy loam soil under controlled conditions. Seven soil fumigants that are registered in California or are being or have been considered for registration were used in this study: dimethyl disulfide (DMDS mixed with chloropicrin (Pic (79% DMDS and 21% Pic, Tri-Con (50% methyl bromide and 50% Pic, Midas Gold (33% methyl iodide [MI] and 67% Pic, Midas Bronze (50% MI and 50% Pic, Midas (MI, active ingredient [a.i.] 97.8%, Pic (a.i. 99% trichloronitromethane and Pic-Clor 60 (57% Pic and 37% 1,3-dichloropropene [1–3,D]. Dose-response models were calculated for pathogen mortality after 24 hours of exposure to fumigants. Overall, the tested fumigants achieved good efficacy with dosages below the maximum label rate against the tested pathogens. In this study, Pythium ultimum and citrus nematode were sensitive to all the fumigants and Verticillium dahliae was resistant. For most fumigants, California regulations restrict application rates to less than the maximum (federal label rate, meaning that it is possible that the fumigants may not control major plant pathogens. This research provides information on the effectiveness of these alternatives at these lower application rates. The results from this study will help growers optimize application rates for registered fumigants (such as Pic and 1,3-D and will help accelerate the adoption of new fumigants (such as DMDS if they are registered in California.

Improved antifungal activity of barley derived chitinase I gene that overexpress a 32 kDa recombinant chitinase in Escherichia coli host

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Nida Toufiq

Full Text Available Abstract Agricultural crops suffer many diseases, including fungal and bacterial infections, causing significant yield losses. The identification and characterisation of pathogenesis-related protein genes, such as chitinases, can lead to reduction in pathogen growth, thereby increasing tolerance against fungal pathogens. In the present study, the chitinase I gene was isolated from the genomic DNA of Barley (Hordeum vulgare L. cultivar, Haider-93. The isolated DNA was used as template for the amplification of the ∼935 bp full-length chitinase I gene. Based on the sequence of the amplified gene fragment, class I barley chitinase shares 93% amino acid sequence homology with class II wheat chitinase. Interestingly, barley class I chitinase and class II chitinase do not share sequence homology. Furthermore, the amplified fragment was expressed in Escherichia coli Rosetta strain under the control of T7 promoter in pET 30a vector. Recombinant chitinase protein of 35 kDa exhibited highest expression at 0.5 mM concentration of IPTG. Expressed recombinant protein of 35 kDa was purified to homogeneity with affinity chromatography. Following purification, a Western blot assay for recombinant chitinase protein measuring 35 kDa was developed with His-tag specific antibodies. The purified recombinant chitinase protein was demonstrated to inhibit significantly the important phytopathogenic fungi Alternaria solani, Fusarium spp, Rhizoctonia solani and Verticillium dahliae compared to the control at concentrations of 80 µg and 200 µg.

Serving vegetables first: A strategy to increase vegetable consumption in elementary school cafeterias.

Science.gov (United States)

Elsbernd, S L; Reicks, M M; Mann, T L; Redden, J P; Mykerezi, E; Vickers, Z M

2016-01-01

Vegetable consumption in the United States is low despite the wealth of evidence that vegetables play an important role in reducing risk of various chronic diseases. Because eating patterns developed in childhood continue through adulthood, we need to form healthy eating habits in children. The objective of this study was to determine if offering vegetables before other meal components would increase the overall consumption of vegetables at school lunch. We served kindergarten through fifth-grade students a small portion (26-33 g) of a raw vegetable (red and yellow bell peppers) while they waited in line to receive the rest of their lunch meal. They then had the options to take more of the bell peppers, a different vegetable, or no vegetable from the lunch line. We measured the amount of each vegetable consumed by each child. Serving vegetables first greatly increased the number of students eating vegetables. On intervention days most of the vegetables consumed came from the vegetables-first portions. Total vegetable intake per student eating lunch was low because most students chose to not eat vegetables, but the intervention significantly increased this value. Serving vegetables first is a viable strategy to increase vegetable consumption in elementary schools. Long-term implementation of this strategy may have an important impact on healthy eating habits, vegetable consumption, and the health consequences of vegetable intake. Copyright © 2015 Elsevier Ltd. All rights reserved.

Cadmium and zinc response of the fungi Heliscus lugdunensis and Verticillium cf. alboatrum isolated from highly polluted water

International Nuclear Information System (INIS)

Jaeckel, Petra; Krauss, Gerd-Joachim; Krauss, Gudrun

2005-01-01

The aquatic hyphomycete Heliscus lugdunensis and the terrestrial fungus Verticillium cf. alboatrum, both isolated from a highly polluted surface water, were investigated for their tolerance against Cd and Zn. Hl-H4 showed a 50% growth inhibition at 0.1 mM Cd, whereas at 0.7 mM Cd the growth of Va-H4 was only reduced by 30%. The fungi also showed a remarkable difference in their Zn-tolerance. The growth of Va-H4 was not inhibited at 1 mM Zn, whereas for Hl-H4 no growth occurred above 0.3 mM Zn. The biosorption and accumulation capacities for Cd or Zn of both fungi differed between the fungal species. In a 0.1 mM Cd-medium Hl-H4 biosorbed 15-fold and accumulated 39-fold more Cd than Va-H4. Exposure to 0.3 mM Zn resulted in a 13-fold higher biosorption and 11-fold higher accumulation for Hl-H4 than Va-H4. As glutathione (GSH) is known to be involved in the phytochelatin synthesis and other stress related processes we investigated its synthesis. Both fungi increased their synthesis of GSH in response to Cd. For Hl-H4 a concentration of 0.0125 mM Cd, corresponding to an intracellular Cd content of 2.1 nmol Cd mg -1 dw, increased the GSH content, whereas Va-H4 only responded with a higher production of GSH at 1 mM Cd and a concomitant intracellular Cd content of 22.5 nmol Cd mg -1 dw. An increased GSH synthesis under Zn-stress was only detectable for Va-H4 (20 mM)

Post-fire vegetation recovery in Portugal based on spot/vegetation data

Science.gov (United States)

Gouveia, C.; Dacamara, C. C.; Trigo, R. M.

2010-04-01

A procedure is presented that allows identifying large burned scars and the monitoring of vegetation recovery in the years following major fire episodes. The procedure relies on 10-day fields of Maximum Value Composites of Normalized Difference Vegetation Index (MVC-NDVI), with a 1 km×1 km spatial resolution obtained from the VEGETATION instrument. The identification of fire scars during the extremely severe 2003 fire season is performed based on cluster analysis of NDVI anomalies that persist during the vegetative cycle of the year following the fire event. Two regions containing very large burned scars were selected, located in Central and Southwestern Portugal, respectively, and time series of MVC-NDVI analysed before the fire events took place and throughout the post-fire period. It is shown that post-fire vegetation dynamics in the two selected regions may be characterised based on maps of recovery rates as estimated by fitting a monoparametric model of vegetation recovery to MVC-NDVI data over each burned scar. Results indicated that the recovery process in the region located in Central Portugal is mostly related to fire damage rather than to vegetation density before 2003, whereas the latter seems to have a more prominent role than vegetation conditions after the fire episode, e.g. in the case of the region in Southwestern Portugal. These differences are consistent with the respective predominant types of vegetation. The burned area located in Central Portugal is dominated by Pinus Pinaster whose natural regeneration crucially depends on the destruction of seeds present on the soil surface during the fire, whereas the burned scar in Southwestern Portugal was populated by Eucalyptus that may quickly re-sprout from buds after fire. Besides its simplicity, the monoparametric model of vegetation recovery has the advantage of being easily adapted to other low-resolution satellite data, as well as to other types of vegetation indices.

The Impact of the Rise in Vegetable Prices on Vegetable Producer Behavior–Based on the survey of vegetable producers in Jiayu, Hubei Province

Directory of Open Access Journals (Sweden)

Liu Pan

2015-01-01

Full Text Available In order to study the impact of the rise in prices of vegetables on vegetable producers, and to increase the revenue of vegetable producers, this paper does a survey by anonymous sampling questionnaire. Results shows that: most vegetable growers think that vegetable prices should rise and would continue to rise, and that vegetable prices would increase their revenue, thus in the coming year they would expand the planting scale of vegetable variety whose increase rate is the largest in this year. But because of the increase of logistics costs and production costs, some farmers benefit very little from the rising trend of vegetable prices. Most farmers expect too much in the trend estimation of the prices of vegetables and also lack of planning and forward-looking in production, thus the planting area of single variety is often decided by the market of previous year. According to analysis of the impact of the rise in vegetable prices on vegetable producer behavior, this paper gives the following suggestions to increase revenue of vegetable producers: change the mode of thinking, improve rural information platform, and increase capital investment for vegetable production base.

Isolation and propagation of mutations by in vitro culture. Part of a coordinated programme on improvement of vegetatively propagated crops and tree crops through radiation induced mutations

International Nuclear Information System (INIS)

Asahira, T.

1974-01-01

Tuberous roots of two Dahlia cultivars were irradiated with 1000 - 2000 R of x-rays. Chlorophyll and flower colour mutations were scored on M 1 plants and the subsequent vegetatively propagated generations. The project aimed at the development of experimental methods suitable for easy isolation of mutated tissue chimeras deriving from mutagen treatment. In comparing in vivo methods such as leaf bud cutting and root propagation with in vitro methods using explants from leaves and florets, the problems encountered by in vitro culture were so manifold, that this method is not considered to be at present of much promise. These experimental results should not discourage from developing and using in vitro culture methods for mutant isolation in other plant species, particularly those were in vivo adventitious bud techniques are not available. Besides the main result obtained, the following valuable observations were made: a) The genotype used for mutation induction is not only relevant for the number and the spectrum of mutations, but also size of chimerical sectors. b) Flower colour changes, which were investigated in some detail, revealed that pigments were lost as groups e.g. all buteins in one case and all cyanins plus pelargonins in another case. c) When using the leaf bud cutting method the greatest frequency of mutations was recovered from the axillary bud of the leaf, that is at the youngest primordial stage at the time of mutagen treatment

Correlation between resistance of eggplant and defense-related ...

African Journals Online (AJOL)

ajl user 1

2012-09-13

Sep 13, 2012 ... verticillium wilt, the activities of defense-related enzymes, and the contents of some biochemical substances of ... mainly divided into blocking theory and toxin theory ..... and researchers have paid attention to verticillium wilt.

Garlic, from Remedy to Stimulant: Evaluation of Antifungal Potential Reveals Diversity in Phytoalexin Allicin Content among Garlic Cultivars; Allicin Containing Aqueous Garlic Extracts Trigger Antioxidants in Cucumber

Science.gov (United States)

Hayat, Sikandar; Cheng, Zhihui; Ahmad, Husain; Ali, Muhammad; Chen, Xuejin; Wang, Mengyi

2016-01-01

Garlic has the charisma of a potent remedy and holds its repute of a therapeutic panacea since the dawn of civilization. An integrated approach was adopted to evaluate the genetic diversity among Chinese garlic cultivars for their antifungal potency as well as allicin content distribution and, furthermore; a bioassay was performed to study the bio-stimulation mechanism of aqueous garlic extracts (AGE) in the growth and physiology of cucumber (Cucumis sativus). Initially, 28 garlic cultivars were evaluated against four kinds of phytopathogenic fungi; Fusarium oxysporum, Botrytis cinerea, Verticillium dahliae and Phytophthora capsici, respectively. A capricious antifungal potential among the selected garlic cultivars was observed. HPLC fingerprinting and quantification confirmed diversity in allicin abundance among the selected cultivars. Cultivar G025, G064, and G074 had the highest allicin content of 3.98, 3.7, and 3.66 mg g-1, respectively, whereas G110 was found to have lowest allicin content of 0.66 mg g-1. Cluster analysis revealed three groups on the basis of antifungal activity and allicin content among the garlic cultivars. Cultivar G025, G2011-4, and G110 were further evaluated to authenticate the findings through different solvents and shelf life duration and G025 had the strongest antifungal activity in all conditions. minimum inhibitory concentration and minimum fungicidal concentration of Allicin aqueous standard (AAS) and AGE showed significant role of allicin as primary antifungal substance of AGE. Leaf disk bioassay against P. capsici and V. dahliae to comparatively study direct action of AGE and AAS during infection process employing eggplant and pepper leaves showed a significant reduction in infection percentage. To study the bioactivity of AGE, a bioassay was performed using cucumber seedlings and results revealed that AGE is biologically active inside cucumber seedlings and alters the defense mechanism of the plant probably activating reactive

European Vegetation Archive (EVA): an integrated database of European vegetation plots

DEFF Research Database (Denmark)

Chytrý, M; Hennekens, S M; Jiménez-Alfaro, B

2015-01-01

vegetation- plot databases on a single software platform. Data storage in EVA does not affect on-going independent development of the contributing databases, which remain the property of the data contributors. EVA uses a prototype of the database management software TURBOVEG 3 developed for joint management......The European Vegetation Archive (EVA) is a centralized database of European vegetation plots developed by the IAVS Working Group European Vegetation Survey. It has been in development since 2012 and first made available for use in research projects in 2014. It stores copies of national and regional...... data source for large-scale analyses of European vegetation diversity both for fundamental research and nature conservation applications. Updated information on EVA is available online at http://euroveg.org/eva-database....

Multispectral imaging as a potential tool for seed health testing of spinach (Spinacia oleracea L.)

DEFF Research Database (Denmark)

Olesen, M. Halkjaer; Carstensen, Jens Michael; Boelt, B.

2011-01-01

University. Our study demonstrates that multispectral imaging with wavelengths ranging from 395-970 nm can be used to distinguish between uninfected spinach seeds and seeds infected with Verticillium spp., Fusarium spp., Stemphylium botryosum, Cladosporium spp. and Alternaria alternata. Analytical separation...... using only NIR gave a separation of 26-88% between uninfected and Fusarium spp. infected seeds. Alternaria alternata and Fusarium spp. could be distinguished from each other and from Cladosporium spp., Verticillium spp. and Stemphylium spp. Separation of Cladosporium spp., Verticillium spp....... and Stemphylium spp. needs further development before practical application....

Post-fire vegetation recovery in Portugal based ewline on spot/vegetation data

Directory of Open Access Journals (Sweden)

C. Gouveia

2010-04-01

Full Text Available A procedure is presented that allows identifying large burned scars and the monitoring of vegetation recovery in the years following major fire episodes. The procedure relies on 10-day fields of Maximum Value Composites of Normalized Difference Vegetation Index (MVC-NDVI, with a 1 km×1 km spatial resolution obtained from the VEGETATION instrument. The identification of fire scars during the extremely severe 2003 fire season is performed based on cluster analysis of NDVI anomalies that persist during the vegetative cycle of the year following the fire event. Two regions containing very large burned scars were selected, located in Central and Southwestern Portugal, respectively, and time series of MVC-NDVI analysed before the fire events took place and throughout the post-fire period. It is shown that post-fire vegetation dynamics in the two selected regions may be characterised based on maps of recovery rates as estimated by fitting a monoparametric model of vegetation recovery to MVC-NDVI data over each burned scar. Results indicated that the recovery process in the region located in Central Portugal is mostly related to fire damage rather than to vegetation density before 2003, whereas the latter seems to have a more prominent role than vegetation conditions after the fire episode, e.g. in the case of the region in Southwestern Portugal. These differences are consistent with the respective predominant types of vegetation. The burned area located in Central Portugal is dominated by Pinus Pinaster whose natural regeneration crucially depends on the destruction of seeds present on the soil surface during the fire, whereas the burned scar in Southwestern Portugal was populated by Eucalyptus that may quickly re-sprout from buds after fire. Besides its simplicity, the monoparametric model of vegetation recovery has the advantage of being easily adapted to other low-resolution satellite data, as well as to other types of vegetation

Method of producing vegetable puree

DEFF Research Database (Denmark)

2004-01-01

A process for producing a vegetable puree, comprising the sequential steps of: a)crushing, chopping or slicing the vegetable into pieces of 1 to 30 mm; b) blanching the vegetable pieces at a temperature of 60 to 90°C; c) contacted the blanched vegetable pieces with a macerating enzyme activity; d......) blending the macerated vegetable pieces and obtaining a puree....

Estimating vegetation dryness to optimize fire risk assessment with spot vegetation satellite data in savanna ecosystems

Science.gov (United States)

Verbesselt, J.; Somers, B.; Lhermitte, S.; van Aardt, J.; Jonckheere, I.; Coppin, P.

2005-10-01

The lack of information on vegetation dryness prior to the use of fire as a management tool often leads to a significant deterioration of the savanna ecosystem. This paper therefore evaluated the capacity of SPOT VEGETATION time-series to monitor the vegetation dryness (i.e., vegetation moisture content per vegetation amount) in order to optimize fire risk assessment in the savanna ecosystem of Kruger National Park in South Africa. The integrated Relative Vegetation Index approach (iRVI) to quantify the amount of herbaceous biomass at the end of the rain season and the Accumulated Relative Normalized Difference vegetation index decrement (ARND) related to vegetation moisture content were selected. The iRVI and ARND related to vegetation amount and moisture content, respectively, were combined in order to monitor vegetation dryness and optimize fire risk assessment in the savanna ecosystems. In situ fire activity data was used to evaluate the significance of the iRVI and ARND to monitor vegetation dryness for fire risk assessment. Results from the binary logistic regression analysis confirmed that the assessment of fire risk was optimized by integration of both the vegetation quantity (iRVI) and vegetation moisture content (ARND) as statistically significant explanatory variables. Consequently, the integrated use of both iRVI and ARND to monitor vegetation dryness provides a more suitable tool for fire management and suppression compared to other traditional satellite-based fire risk assessment methods, only related to vegetation moisture content.

Evaluation of vegetation cover using the normalized difference vegetation index (NDVI

Directory of Open Access Journals (Sweden)

Gabriela Camargos Lima

2013-08-01

Full Text Available Soil loss by water erosion is the main cause of soil degradation in Brazil. However, erosion can be reduced by the presence of vegetation. The Normalized Difference Vegetation Index (NDVI makes it possible to identify the vegetative vigor of crops or natural vegetation which facilities the identification of areas with vegetation covers. This information is very important in identifying the phenomena which might be occurring in a particular area, especially those related to soil degradation by water erosion. Thus, the aim of this work was to assess the canopy cover by using NDVI, checking the image accuracy using the Coverage Index (CI based on the Stocking method, in the Sub-basin of Posses, which belongs to the Cantareira System, located in the Extrema municipality, Minas Gerais, Brazil. Landsat-5 TM images were used. The sub-basin of Posses was very altered in comparison to the surrounding areas. The NDVI technique proved to be a suitable tool to assess the uses that occur in the sub-basin of Posses, as validated by the Stocking methodology. The map derived from NDVI allowed the geographic distribution of different land uses to be observed and allowed for the identification of critical areas in relation to vegetation cover as well. This finding can be used to optimize efforts to recover and protect soil in areas with bare soil and degraded pasture, in order to reduce environmental degradation. The CI has not exceeded 40% for land use classes that occur in the majority of the sub-basin (91%, except in areas of woody vegetation.

European Vegetation Archive (EVA)

NARCIS (Netherlands)

Chytrý, Milan; Hennekens, S.M.; Jiménez-Alfaro, Borja; Schaminée, J.H.J.; Haveman, Rense; Janssen, J.A.M.

2016-01-01

The European Vegetation Archive (EVA) is a centralized database of European vegetation plots developed by the IAVS Working Group European Vegetation Survey. It has been in development since 2012 and first made available for use in research projects in 2014. It stores copies of national and

Children and vegetables: strategies to increase children’s liking and intake of vegetables

NARCIS (Netherlands)

Wild, de V.W.T.

2015-01-01

Background and aim

Children’s vegetable intake is far below that recommended. Despite increased awareness of the importance of vegetable consumption for health, it remains challenging to improve children’s vegetable intake. Since food preferences are central to

A New Synthetic Amphiploid (AADDAA) between Gossypium hirsutum and G. arboreum Lays the Foundation for Transferring Resistances to Verticillium and Drought

Science.gov (United States)

Chen, Yu; Wang, Yingying; Zhao, Ting; Yang, Jianwei; Feng, Shouli; Nazeer, Wajad; Zhang, Tianzhen; Zhou, Baoliang

2015-01-01

Gossypium arboreum, a cultivated cotton species (2n = 26, AA) native to Asia, possesses invaluable characteristics unavailable in the tetraploid cultivated cotton gene pool, such as resistance to pests and diseases and tolerance to abiotic stresses. However, it is quite difficult to transfer favorable traits into Upland cotton through conventional methods due to the cross-incompatibility of G. hirsutum (2n = 52, AADD) and G. arboreum. Here, we improved an embryo rescue technique to overcome the cross-incompatibility between these two parents for transferring favorable genes from G. arboreum into G. hirsutum. Our results indicate that MSB2K supplemented with 0.5 mgl-1 kinetin and 250 mg-1 casein hydrolysate is an efficient initial medium for rescuing early (3 d after pollination) hybrid embryos. Eight putative hybrids were successfully obtained, which were further verified and characterized by cytology, molecular markers and morphological analysis. The putative hybrids were subsequently treated with different concentrations of colchicine solution to double their chromosomes. The results demonstrate that four putative hybrid plants were successfully chromosome-doubled by treatment with 0.1% colchicine for 24 h and become amphiploid, which were confirmed by cytological observation, self-fertilization and backcrossing. Preliminary assessments of resistance at seedling stage indicate that the synthetic amphiploid showed highly resistant to Verticillium and drought. The synthetic amphiploid between G. hirsutum × G. arboreum would lay the foundation for developing G. arboreum-introgressed lines with the uniform genetic background of G. hirsutum acc TM-1, which would greatly enhance and simplify the mining, isolation, characterization, cloning and use of G. arboreum-specific desirable genes in future cotton breeding programs. PMID:26061996

Formation of banded vegetation patterns resulted from interactions between sediment deposition and vegetation growth.

Science.gov (United States)

Huang, Tousheng; Zhang, Huayong; Dai, Liming; Cong, Xuebing; Ma, Shengnan

2018-03-01

This research investigates the formation of banded vegetation patterns on hillslopes affected by interactions between sediment deposition and vegetation growth. The following two perspectives in the formation of these patterns are taken into consideration: (a) increased sediment deposition from plant interception, and (b) reduced plant biomass caused by sediment accumulation. A spatial model is proposed to describe how the interactions between sediment deposition and vegetation growth promote self-organization of banded vegetation patterns. Based on theoretical and numerical analyses of the proposed spatial model, vegetation bands can result from a Turing instability mechanism. The banded vegetation patterns obtained in this research resemble patterns reported in the literature. Moreover, measured by sediment dynamics, the variation of hillslope landform can be described. The model predicts how treads on hillslopes evolve with the banded patterns. Thus, we provide a quantitative interpretation for coevolution of vegetation patterns and landforms under effects of sediment redistribution. Copyright © 2018. Published by Elsevier Masson SAS.

Irradiation of dehydrated vegetables

International Nuclear Information System (INIS)

Esterhuyse, A; Esterhuizen, T.

1985-01-01

The reason for radurization was to decreased the microbial count of dehydrated vegetables. The average absorbed irradiation dose range between 2kGy and 15kGy. The product catagories include a) Green vegetables b) White vegetables c) Powders of a) and b). The microbiological aspects were: Declining curves for the different products of T.P.C., Coliforms, E. Coli, Stap. areus, Yeast + Mold at different doses. The organoleptical aspects were: change in taste, flavour, texture, colour and moisture. The aim is the marketing of irradiated dehydrated vegetables national and international basis

Vegetable Genetic Resources in China

Directory of Open Access Journals (Sweden)

Haiping WANG

2018-03-01

Full Text Available China is recognized as an important region for plant biodiversity based on its vast and historical collection of vegetable germplasm. The aim of this review is to describe the exploration status of vegetable genetic resources in China, including their collection, preservation, evaluation, and utilization. China has established a number of national-level vegetable genetic resources preservation units, including the National Mid-term Genebank for Vegetable Germplasm Resources, the National Germplasm Repository for Vegetatively-Propagated Vegetables, and the National Germplasm Repository for Aquatic Vegetables. In 2015, at least 36 000 accessions were collected and preserved in these units. In the past decade, 44 descriptors and data standards for different species have been published, and most accessions have been evaluated for screening the germplasms for specific important traits such as morphological characteristics, disease resistance, pest resistance, and stress tolerance. Moreover, the genetic diversity and evolution of some vegetable germplasms have been evaluated at the molecular level. Recently, more than 1 000 accessions were distributed to researchers and breeders each year by various means for vegetable research and production. However, additional wild-relative and abroad germplasms from other regions need to be collected and preserved in the units to expand genetic diversity. Furthermore, there is a need to utilize advanced techniques to better understand the background and genetic diversity of a wide range of vegetable genetic resources. This review will provide agricultural scientists’ insights into the genetic diversity in China and provide information on the distribution and potential utilization of these valuable genetic resources. Keywords: vegetable, genetic resource, preservation, evaluation, utilization

Heavy metals in green vegetables and soils from vegetable gardens ...

African Journals Online (AJOL)

Edible portions of five varieties of green vegetables, namely amaranth, chinese cabbage, cowpea leaves, leafy cabbage and pumpkin leaves, collected from several areas in Dar es Salaam, were analyzed for lead, cadmium, chromium, zinc, nickel and copper. Except for zinc, the levels of heavy metals in the vegetables ...

Kandungan Inulin Dari Umbi Dahliasp Yang Ditanam Pada Jenis Tanah Vertisol, Inceptisol Dan Andisol

OpenAIRE

Iskandar, Yetti Mulyati; Pudjiraharti, Sri; Ratnaningrum, Diah

2014-01-01

Tujuan dari penelitian ini adalah menentukan kandungan inulin dari umbi Dahlia sp yang ditanam didaerah sejuk seperti Cianjur, Lembang dan Sukabumi pada jenis tanah yang berbeda dalam rangka rencana produksi di Jawa Barat. Inulin adalah oligosacharida yang terjadi secara alami dengan komposisi gabungan fruktooligosacharida dari oligomer dengan derajat polimerisasi yang berbeda. Umbi dahlia dari bunga berwarna ungu, merah, kuning dan putih yang ditanam di tiga lokasi digunaka...

The Role of IDO in Muc1 Targeted Immunotherapy

Science.gov (United States)

2013-01-01

Besmer*, Teresa L. Tinder , Lopamudra Das Roy, Joseph Lustgarten, Sandra J. Gendler, Pinku Mukherjee Intratumoral Delivery of CpG-Conjugated Anti...3073-87. Epub 2012 Jan 11. PMID: 22238308 3. Mahnaz Sahraei , Lopamudra Das Roy , Jennifer Curry , Teresa Tinder , Sritama Nath , Dahlia M...10.1038/onc.2011.651 4. Dahlia M. Besmer , Dr. Jennifer M. Curry , Dr. Lopamudra D. Roy , Ms. Teresa L. Tinder , Ms. Mahnaz M. Sahraei , Dr

Review of Vegetable Market Development in China

Institute of Scientific and Technical Information of China (English)

Chaoping; LUO; Yuandong; NI; Qiong; ZHAI

2013-01-01

This paper has reviewed vegetable market development from vegetable circulation system, the develop history of the liberalize vegetable market and the growth of the vegetable wholesale market in China. From the development of vegetables market in China and its characteristics: the development of vegetable market in China is related to vegetable market system, the change of institution, some technology development and infrastructure. this paper has put forward some related measures to perfect the vegetable market and improve the vegetable circulation efficiency in China.

Monitoring of vegetation dynamics and assessing vegetation response to drought in the Iberian Peninsula

Energy Technology Data Exchange (ETDEWEB)

Garcia-Haro, F. J.; Moreno, A.; Perez-Hoyos, A.; Gilabert, M. A.; Melia, J.; Belda, F.; Poquet, D.; Martinez, B.; Verger, A.

2009-07-01

Monitoring the vegetation activity over long time-scales is necessary to discern ecosystem response to climate variability. Spatial and temporally consistent estimates of the biophysical variables such as fractional vegetation cover (FVC) and leaf area index (LAI) have been obtained in the context of DULCINEA Project. We used long-term monthly climate statistics to build simple climatic indices (SPI, moisture index) at different time scales. From these indices, we estimated that the climatic disturbances affected both the growing season and the total amount of vegetation. This implies that the anomaly of vegetation cover is a good indicator of moisture condition and can be an important data source when used for detecting an monitoring drought in the Iberian Peninsula. The impact of climate variability on the vegetation dynamics has shown not to be the same for every region. We concluded that the relationships between vegetation anomaly and moisture availability are significant for the arid and semiarid areas. (Author) 6 refs.

Monitoring of vegetation dynamics and assessing vegetation response to drought in the Iberian Peninsula

International Nuclear Information System (INIS)

Garcia-Haro, F. J.; Moreno, A.; Perez-Hoyos, A.; Gilabert, M. A.; Melia, J.; Belda, F.; Poquet, D.; Martinez, B.; Verger, A.

2009-01-01

Monitoring the vegetation activity over long time-scales is necessary to discern ecosystem response to climate variability. Spatial and temporally consistent estimates of the biophysical variables such as fractional vegetation cover (FVC) and leaf area index (LAI) have been obtained in the context of DULCINEA Project. We used long-term monthly climate statistics to build simple climatic indices (SPI, moisture index) at different time scales. From these indices, we estimated that the climatic disturbances affected both the growing season and the total amount of vegetation. This implies that the anomaly of vegetation cover is a good indicator of moisture condition and can be an important data source when used for detecting an monitoring drought in the Iberian Peninsula. The impact of climate variability on the vegetation dynamics has shown not to be the same for every region. We concluded that the relationships between vegetation anomaly and moisture availability are significant for the arid and semiarid areas. (Author) 6 refs.

The Efficacy of Performance Appraisal to Increase Employees’ Job Satisfaction

Directory of Open Access Journals (Sweden)

Iranita Hervi Mahardayani

2016-08-01

Full Text Available This research aims to find out the effectiveness of implementation performance appraisal toward job satisfaction of employees UMKM Bordir Dahlia in Kabupaten Kudus. Sampling was done by using population studies to 16 employees of UMKM embroidery Dahlia Kudus. The type of this research is quantitative with pra experiment approached by One group pre and posttest design method. Data analysis on this research using non parametric with different test techniques Wilcoxon t-tes. The analysis result of different test scale job satisfaction before and after handling obtained Z = - 1,268, with p = 0,205 (p>0,05, so that there is no differences on job satisfaction on employees of embroidery Dahlia before and after treatment by giving performance appraisal. It means treatment by giving performance appraisal unable to increase employees job satisfaction. Therefore, the hypothesis on this research is rejected.

Balkan Vegetation Database

NARCIS (Netherlands)

Vassilev, Kiril; Pedashenko, Hristo; Alexandrova, Alexandra; Tashev, Alexandar; Ganeva, Anna; Gavrilova, Anna; Gradevska, Asya; Assenov, Assen; Vitkova, Antonina; Grigorov, Borislav; Gussev, Chavdar; Filipova, Eva; Aneva, Ina; Knollová, Ilona; Nikolov, Ivaylo; Georgiev, Georgi; Gogushev, Georgi; Tinchev, Georgi; Pachedjieva, Kalina; Koev, Koycho; Lyubenova, Mariyana; Dimitrov, Marius; Apostolova-Stoyanova, Nadezhda; Velev, Nikolay; Zhelev, Petar; Glogov, Plamen; Natcheva, Rayna; Tzonev, Rossen; Boch, Steffen; Hennekens, Stephan M.; Georgiev, Stoyan; Stoyanov, Stoyan; Karakiev, Todor; Kalníková, Veronika; Shivarov, Veselin; Russakova, Veska; Vulchev, Vladimir

2016-01-01

The Balkan Vegetation Database (BVD; GIVD ID: EU-00-019; http://www.givd.info/ID/EU-00- 019) is a regional database that consists of phytosociological relevés from different vegetation types from six countries on the Balkan Peninsula (Albania, Bosnia and Herzegovina, Bulgaria, Kosovo, Montenegro

Microbiological Spoilage of Fruits and Vegetables

Science.gov (United States)

Barth, Margaret; Hankinson, Thomas R.; Zhuang, Hong; Breidt, Frederick

Consumption of fruit and vegetable products has dramatically increased in the United States by more than 30% during the past few decades. It is also estimated that about 20% of all fruits and vegetables produced is lost each year due to spoilage. The focus of this chapter is to provide a general background on microbiological spoilage of fruit and vegetable products that are organized in three categories: fresh whole fruits and vegetables, fresh-cut fruits and vegetables, and fermented or acidified vegetable products. This chapter will address characteristics of spoilage microorganisms associated with each of these fruit and vegetable categories including spoilage mechanisms, spoilage defects, prevention and control of spoilage, and methods for detecting spoilage microorganisms.

Lake Bathymetric Aquatic Vegetation

Data.gov (United States)

Minnesota Department of Natural Resources — Aquatic vegetation represented as polygon features, coded with vegetation type (emergent, submergent, etc.) and field survey date. Polygons were digitized from...

Effects of vegetation structure on biomass accumulation in a Balanced Optimality Structure Vegetation Model (BOSVM v1.0

Directory of Open Access Journals (Sweden)

Z. Yin

2014-05-01

Full Text Available A myriad of interactions exist between vegetation and local climate for arid and semi-arid regions. Vegetation function, structure and individual behavior have large impacts on carbon–water–energy balances, which consequently influence local climate variability that, in turn, feeds back to the vegetation. In this study, a conceptual vegetation structure scheme is formulated and tested in the new Balanced Optimality Structure Vegetation Model (BOSVM to explore the importance of vegetation structure and vegetation adaptation to water stress on equilibrium biomass states. Surface energy, water and carbon fluxes are simulated for a range of vegetation structures across a precipitation gradient in West Africa and optimal vegetation structures that maximize biomass for each precipitation regime are determined. Two different strategies of vegetation adaptation to water stress are included. Under dry conditions vegetation tries to maximize the water use efficiency and leaf area index as it tries to maximize carbon gain. However, a negative feedback mechanism in the vegetation–soil water system is found as the vegetation also tries to minimize its cover to optimize the surrounding bare ground area from which water can be extracted, thereby forming patches of vertical vegetation. Under larger precipitation, a positive feedback mechanism is found in which vegetation tries to maximize its cover as it then can reduce water loss from bare soil while having maximum carbon gain due to a large leaf area index. The competition between vegetation and bare soil determines a transition between a "survival" state to a "growing" state.

Add More Vegetables to Your Day: 10 Tips to Help You Eat More Vegetables

Science.gov (United States)

... sip on some vegetable soup Heat it and eat it. Try tomato, butternut squash, or garden vegetable soup. Look for reduced- or low-sodium soups. Make your own soups with a low-sodium broth and your favorite vegetables. 8 while you’re out If dinner is away from home, no need to worry. ...

Vegetation index anomaly response to varying lengths of drought across vegetation and climatic gradients in Hawaii

Science.gov (United States)

Lucas, M.; Miura, T.; Trauernicht, C.; Frazier, A. G.

2017-12-01

A drought which results in prolonged and extended deficit in naturally available water supply and creates multiple stresses across ecosystems is classified as an ecological drought. Detecting and understanding the dynamics and response of such droughts in tropical systems, specifically across various vegetation and climatic gradients is fairly undetermined, yet increasingly important for better understandings of the ecological effects of drought. To understanding the link between what lengths and intensities of known meteorological drought triggers detectable ecological vegetation responses, a landscape scale regression analysis evaluating the response (slope) and relationship strength (R-squared) of several cumulative SPI (standard precipitation index) lengths(1, 3, 6, 12, 18, 24, 36, 48, and 60 month), to various satellite derived monthly vegetation indices anomalies (NDVI, EVI, EVI2, and LSWI) was performed across a matrix of dominant vegetation covers (grassland, shrubland, and forest) and climatic moisture zones (arid, dry, mesic, and wet). The nine different SPI lags across these climactic and vegetation gradients was suggest that stronger relationships and steeper slopes were found in dryer climates (across all vegetation covers) and finer vegetation types (across all moisture zones). Overall NDVI, EVI and EVI2 showed the best utility in these dryer climatic zones across all vegetation types. Within arid and dry areas "best" fits showed increasing lengths of cumulative SPI were with increasing vegetation coarseness respectively. Overall these findings suggest that rainfall driven drought may have a stronger impact on the ecological condition of vegetation in water limited systems with finer vegetation types ecologically responding more rapidly to meteorological drought events than coarser woody vegetation systems. These results suggest that previously and newly documented trends of decreasing rainfall and increasing drought in Hawaiian drylands may have

Vegetation extraction from high-resolution satellite imagery using the Normalized Difference Vegetation Index (NDVI)

Science.gov (United States)

AlShamsi, Meera R.

2016-10-01

Over the past years, there has been various urban development all over the UAE. Dubai is one of the cities that experienced rapid growth in both development and population. That growth can have a negative effect on the surrounding environment. Hence, there has been a necessity to protect the environment from these fast pace changes. One of the major impacts this growth can have is on vegetation. As technology is evolving day by day, there is a possibility to monitor changes that are happening on different areas in the world using satellite imagery. The data from these imageries can be utilized to identify vegetation in different areas of an image through a process called vegetation detection. Being able to detect and monitor vegetation is very beneficial for municipal planning and management, and environment authorities. Through this, analysts can monitor vegetation growth in various areas and analyze these changes. By utilizing satellite imagery with the necessary data, different types of vegetation can be studied and analyzed, such as parks, farms, and artificial grass in sports fields. In this paper, vegetation features are detected and extracted through SAFIY system (i.e. the Smart Application for Feature extraction and 3D modeling using high resolution satellite ImagerY) by using high-resolution satellite imagery from DubaiSat-2 and DEIMOS-2 satellites, which provide panchromatic images of 1m resolution and spectral bands (red, green, blue and near infrared) of 4m resolution. SAFIY system is a joint collaboration between MBRSC and DEIMOS Space UK. It uses image-processing algorithms to extract different features (roads, water, vegetation, and buildings) to generate vector maps data. The process to extract green areas (vegetation) utilize spectral information (such as, the red and near infrared bands) from the satellite images. These detected vegetation features will be extracted as vector data in SAFIY system and can be updated and edited by end-users, such as

Vegetables and other core food groups

NARCIS (Netherlands)

Poelman, Astrid A.M.; Delahunty, Conor M.; Graaf, de Kees

2017-01-01

Vegetables are the food category least liked by children. This research investigated the sensory properties of vegetables vis-a-vis other core foods that comprise children's diets, to determine to what degree low acceptance of vegetables can be attributed to sensory properties. Vegetables (n =

18 CFR 1304.203 - Vegetation management.

Science.gov (United States)

2010-04-01

... 18 Conservation of Power and Water Resources 2 2010-04-01 2010-04-01 false Vegetation management...-Owned Residential Access Shoreland § 1304.203 Vegetation management. No vegetation management shall be approved on TVA-owned Residential Access Shoreland until a Vegetation Management Plan meeting the...

Recall of vegetable eating affects future predicted enjoyment and choice of vegetables in British University undergraduate students.

Science.gov (United States)

Robinson, Eric; Blissett, Jackie; Higgs, Suzanne

2011-10-01

Predictions about enjoyment of future experiences are influenced by recalling similar past experiences. However, little is known about the relationship between hedonic memories of past eating episodes and future eating behavior. We investigated recall of previous experiences of eating vegetables and the effect of recall on future predicted liking for and consumption of vegetables. British University undergraduate students were asked to retrieve memories of previous occasions when they ate vegetables and were asked to rate how enjoyable those experiences were (Study 1, n=54). The effect of different types of memory recall (including vegetable eating recall) and visualization of someone else eating vegetables (to control for priming effects) on predicted likelihood of choosing vegetables and predicted enjoyment of eating vegetables was examined (Study 2, n=95). Finally, the effect of recalling vegetable eating memories on actual food choice from a buffet was assessed (Study 3, n=63). It is reported that people recall positive memories of past vegetable consumption (Precall of a personal nonfood memory, a nonvegetable food memory, or visualization of someone else enjoying eating vegetables (increase of approximately 70% in vegetable portion size compared to controls). The results suggest that recall of previous eating experiences could be a potential strategy for altering food choices. Copyright © 2011 American Dietetic Association. Published by Elsevier Inc. All rights reserved.

Comparison of vegetation roughness descriptions

NARCIS (Netherlands)

Augustijn, Dionysius C.M.; Huthoff, Freek; van Velzen, E.H.; Altinakar, M.S.; Kokpinar, M.A.; Aydin, I.; Cokgor, S.; Kirkgoz, S.

2008-01-01

Vegetation roughness is an important parameter in describing flow through river systems. Vegetation impedes the flow, which affects the stage-discharge curve and may increase flood risks. Roughness is often used as a calibration parameter in river models, however when vegetation is allowed to

Vegetable Production System (Veggie)

Data.gov (United States)

National Aeronautics and Space Administration — The Vegetable Production System (Veggie) was developed to be a simple, easily stowed, high growth volume, low resource facility capable of producing fresh vegetables...

Hidden vegetables: an effective strategy to reduce energy intake and increase vegetable intake in adults.

Science.gov (United States)

Blatt, Alexandria D; Roe, Liane S; Rolls, Barbara J

2011-04-01

The overconsumption of energy-dense foods leads to excessive energy intakes. The substitution of low-energy-dense vegetables for foods higher in energy density can help decrease energy intakes but may be difficult to implement if individuals dislike the taste of vegetables. We investigated whether incorporating puréed vegetables to decrease the energy density of entrées at multiple meals reduced daily energy intakes and increased daily vegetable intakes. In this crossover study, 20 men and 21 women ate ad libitum breakfast, lunch, and dinner in the laboratory once a week for 3 wk. Across conditions, entrées at meals varied in energy density from standard versions (100% condition) to reduced versions (85% and 75% conditions) by the covert incorporation of 3 or 4.5 times the amount of puréed vegetables. Entrées were accompanied by unmanipulated side dishes. Participants rated their hunger and fullness before and after meals. Subjects consumed a consistent weight of foods across conditions of energy density; thus, the daily energy intake significantly decreased by 202 ± 60 kcal in the 85% condition (P kcal in the 75% condition (P Daily vegetable consumption significantly increased from 270 ± 17 g of vegetables in the 100% condition to 487 ± 25 g of vegetables in the 75% condition (P < 0.0001). Despite the decreased energy intake, ratings of hunger and fullness did not significantly differ across conditions. Entrées were rated as similar in palatability across conditions. Large amounts of puréed vegetables can be incorporated into various foods to decrease the energy density. This strategy can lead to substantial reductions in energy intakes and increases in vegetable intakes. This trial was registered at clinicaltrials.gov as NCT01165086.

Development of indicators of vegetation recovery based on time series analysis of SPOT Vegetation data

Science.gov (United States)

Lhermitte, S.; Tips, M.; Verbesselt, J.; Jonckheere, I.; Van Aardt, J.; Coppin, Pol

2005-10-01

Large-scale wild fires have direct impacts on natural ecosystems and play a major role in the vegetation ecology and carbon budget. Accurate methods for describing post-fire development of vegetation are therefore essential for the understanding and monitoring of terrestrial ecosystems. Time series analysis of satellite imagery offers the potential to quantify these parameters with spatial and temporal accuracy. Current research focuses on the potential of time series analysis of SPOT Vegetation S10 data (1999-2001) to quantify the vegetation recovery of large-scale burns detected in the framework of GBA2000. The objective of this study was to provide quantitative estimates of the spatio-temporal variation of vegetation recovery based on remote sensing indicators. Southern Africa was used as a pilot study area, given the availability of ground and satellite data. An automated technique was developed to extract consistent indicators of vegetation recovery from the SPOT-VGT time series. Reference areas were used to quantify the vegetation regrowth by means of Regeneration Indices (RI). Two kinds of recovery indicators (time and value- based) were tested for RI's of NDVI, SR, SAVI, NDWI, and pure band information. The effects of vegetation structure and temporal fire regime features on the recovery indicators were subsequently analyzed. Statistical analyses were conducted to assess whether the recovery indicators were different for different vegetation types and dependent on timing of the burning season. Results highlighted the importance of appropriate reference areas and the importance of correct normalization of the SPOT-VGT data.

Encouraging children to eat vegetables

OpenAIRE

Buh, Alenka

2014-01-01

It is important for children to maintain a healthy and balanced diet throughout their childhood and youth. Children tend to skip vegetables in their meals as they are not much liked; the tastes of vegetables are also highly specific and each individual has to get used to them by repeated tasting. The aim of this undergraduate thesis was to analyse how often children eat vegetables, which types of vegetables they like and which they do not, to determine if the executed method of pedagogica...

The Effector SPRYSEC-19 of Globodera rostochiensis Suppresses CC-NB-LRR-Mediated Disease Resistance in Plants1[C][W][OA

Science.gov (United States)

Postma, Wiebe J.; Slootweg, Erik J.; Rehman, Sajid; Finkers-Tomczak, Anna; Tytgat, Tom O.G.; van Gelderen, Kasper; Lozano-Torres, Jose L.; Roosien, Jan; Pomp, Rikus; van Schaik, Casper; Bakker, Jaap; Goverse, Aska; Smant, Geert

2012-01-01

The potato cyst nematode Globodera rostochiensis invades roots of host plants where it transforms cells near the vascular cylinder into a permanent feeding site. The host cell modifications are most likely induced by a complex mixture of proteins in the stylet secretions of the nematodes. Resistance to nematodes conferred by nucleotide-binding-leucine-rich repeat (NB-LRR) proteins usually results in a programmed cell death in and around the feeding site, and is most likely triggered by the recognition of effectors in stylet secretions. However, the actual role of these secretions in the activation and suppression of effector-triggered immunity is largely unknown. Here we demonstrate that the effector SPRYSEC-19 of G. rostochiensis physically associates in planta with the LRR domain of a member of the SW5 resistance gene cluster in tomato (Lycopersicon esculentum). Unexpectedly, this interaction did not trigger defense-related programmed cell death and resistance to G. rostochiensis. By contrast, agroinfiltration assays showed that the coexpression of SPRYSEC-19 in leaves of Nicotiana benthamiana suppresses programmed cell death mediated by several coiled-coil (CC)-NB-LRR immune receptors. Furthermore, SPRYSEC-19 abrogated resistance to Potato virus X mediated by the CC-NB-LRR resistance protein Rx1, and resistance to Verticillium dahliae mediated by an unidentified resistance in potato (Solanum tuberosum). The suppression of cell death and disease resistance did not require a physical association of SPRYSEC-19 and the LRR domains of the CC-NB-LRR resistance proteins. Altogether, our data demonstrated that potato cyst nematodes secrete effectors that enable the suppression of programmed cell death and disease resistance mediated by several CC-NB-LRR proteins in plants. PMID:22904163

The effector SPRYSEC-19 of Globodera rostochiensis suppresses CC-NB-LRR-mediated disease resistance in plants.

Science.gov (United States)

Postma, Wiebe J; Slootweg, Erik J; Rehman, Sajid; Finkers-Tomczak, Anna; Tytgat, Tom O G; van Gelderen, Kasper; Lozano-Torres, Jose L; Roosien, Jan; Pomp, Rikus; van Schaik, Casper; Bakker, Jaap; Goverse, Aska; Smant, Geert

2012-10-01

The potato cyst nematode Globodera rostochiensis invades roots of host plants where it transforms cells near the vascular cylinder into a permanent feeding site. The host cell modifications are most likely induced by a complex mixture of proteins in the stylet secretions of the nematodes. Resistance to nematodes conferred by nucleotide-binding-leucine-rich repeat (NB-LRR) proteins usually results in a programmed cell death in and around the feeding site, and is most likely triggered by the recognition of effectors in stylet secretions. However, the actual role of these secretions in the activation and suppression of effector-triggered immunity is largely unknown. Here we demonstrate that the effector SPRYSEC-19 of G. rostochiensis physically associates in planta with the LRR domain of a member of the SW5 resistance gene cluster in tomato (Lycopersicon esculentum). Unexpectedly, this interaction did not trigger defense-related programmed cell death and resistance to G. rostochiensis. By contrast, agroinfiltration assays showed that the coexpression of SPRYSEC-19 in leaves of Nicotiana benthamiana suppresses programmed cell death mediated by several coiled-coil (CC)-NB-LRR immune receptors. Furthermore, SPRYSEC-19 abrogated resistance to Potato virus X mediated by the CC-NB-LRR resistance protein Rx1, and resistance to Verticillium dahliae mediated by an unidentified resistance in potato (Solanum tuberosum). The suppression of cell death and disease resistance did not require a physical association of SPRYSEC-19 and the LRR domains of the CC-NB-LRR resistance proteins. Altogether, our data demonstrated that potato cyst nematodes secrete effectors that enable the suppression of programmed cell death and disease resistance mediated by several CC-NB-LRR proteins in plants.

Isolation and characterization of terpene synthases in cotton (Gossypium hirsutum).

Science.gov (United States)

Yang, Chang-Qing; Wu, Xiu-Ming; Ruan, Ju-Xin; Hu, Wen-Li; Mao, Yin-Bo; Chen, Xiao-Ya; Wang, Ling-Jian

2013-12-01

Cotton plants accumulate gossypol and related sesquiterpene aldehydes, which function as phytoalexins against pathogens and feeding deterrents to herbivorous insects. However, to date little is known about the biosynthesis of volatile terpenes in this crop. Herein is reported that 5 monoterpenes and 11 sesquiterpenes from extracts of a glanded cotton cultivar, Gossypium hirsutum cv. CCRI12, were detected by gas chromatography-mass spectrometry (GC-MS). By EST data mining combined with Rapid Amplification of cDNA Ends (RACE), full-length cDNAs of three terpene synthases (TPSs), GhTPS1, GhTPS2 and GhTPS3 were isolated. By in vitro assays of the recombinant proteins, it was found that GhTPS1 and GhTPS2 are sesquiterpene synthases: the former converted farnesyl pyrophosphate (FPP) into β-caryophyllene and α-humulene in a ratio of 2:1, whereas the latter produced several sesquiterpenes with guaia-1(10),11-diene as the major product. By contrast, GhTPS3 is a monoterpene synthase, which produced α-pinene, β-pinene, β-phellandrene and trace amounts of other monoterpenes from geranyl pyrophosphate (GPP). The TPS activities were also supported by Virus Induced Gene Silencing (VIGS) in the cotton plant. GhTPS1 and GhTPS3 were highly expressed in the cotton plant overall, whereas GhTPS2 was expressed only in leaves. When stimulated by mechanical wounding, Verticillium dahliae (Vde) elicitor or methyl jasmonate (MeJA), production of terpenes and expression of the corresponding synthase genes were induced. These data demonstrate that the three genes account for the biosynthesis of volatile terpenes of cotton, at least of this Upland cotton. Copyright © 2013 Elsevier Ltd. All rights reserved.

Non-edible Oil Cakes as a Novel Substrate for DPA Production and Augmenting Biocontrol Activity of Paecilomyces variotii

Directory of Open Access Journals (Sweden)

Ashwani Kumar

2017-05-01

Full Text Available The present study investigated the use of waste non-edible oil cakes (Jatropha, Karanja, Neem, and Mahua as a substrate for the growth of Paecilomyces variotii and dipicolinic acid (DPA production. Previous researches proved the efficacy of DPA in suppressing certain pathogens that are deleterious to the plants in the rhizosphere. DPA production was statistical optimized by amending non-edible oil cakes in growing media as nitrogen and sugars (Dextrose, Glucose, and Lactose as carbon source. Plackett-Burman design (PBD, indicated that Jatropha cake, Karanja cake, and Dextrose were the most significant components (p < 0.05 of the media and were further optimized using response surface methodology (RSM. Jatropha cake, Karanja cake, and Dextrose at the concentration of 12.5, 4.5, and 10 g/l, respectively, yielded 250 mg/l of DPA, which was 2.5 fold more than that obtained from basal medium. HPLC analysis of the optimized medium (peak at retention time of 30 min confirmed the enhanced DPA production by P. variotii. The scanning electron microscopy (SEM images showed that optimized medium impose a stress like condition (due to less C:N ratio for the fungus and generated more spores as compared to the basal medium in which carbon source is easily available for the mycelial growth. The antimicrobial activity of the fungal extract was tested and found to be effective even at 10−2 dilution after 72 h against two plant pathogens, Fusarium oxysporum and Verticillium dahlia. Statistical experimental design of this study and the use of non-edible oil cakes as a substrate offer an efficient and viable approach for DPA production by P. variotii.

Special study on vegetative covers

International Nuclear Information System (INIS)

1988-11-01

This report describes the findings of a special study on the use of vegetative covers to stabilize tailings piles for the Uranium Mill Tailings Remedial Action (UMTRA) Project. The principal rationale for using plants would be to establish a dynamic system for controlling water balance. Specifically, vegetation would be used to intercept and transpire precipitation to the atmosphere, rather than allowing water to drain into the tailings and mobilize contaminants. This would facilitate compliance with groundwater standards proposed for the UMTRA Project by the Environmental Protection Agency. The goals of the study were to evaluate the feasibility of using vegetative covers on UMTRA Project piles, define the advantages and disadvantages of vegetative covers, and develop general guidelines for their use when such use seems reasonable. The principal method for the study was to analyze and apply to the UMTRA Project the results of research programs on vegetative covers at other US Department of Energy (DOE) waste management facilities. The study also relied upon observations made of existing stabilized piles at UMTRA Project sites where natural vegetation is growing on the rock-covered surfaces. Water balance and erosion models were also used to quantify the long-term performance of vegetative covers planned for the topslopes of stabilized piles at Grand Junction and Durango, Colorado, two UMTRA Project sites where the decision was made during the course of this special study to use vegetative covers. Elements in the design and construction of the vegetative covers at these two sites are discussed in the report, with explanations of the differing features that reflect differing environmental conditions. 28 refs., 18 figs., 9 tabs

Health Benefits of Fruits and Vegetables1

Science.gov (United States)

Slavin, Joanne L.; Lloyd, Beate

2012-01-01

Fruits and vegetables are universally promoted as healthy. The Dietary Guidelines for Americans 2010 recommend you make one-half of your plate fruits and vegetables. Myplate.gov also supports that one-half the plate should be fruits and vegetables. Fruits and vegetables include a diverse group of plant foods that vary greatly in content of energy and nutrients. Additionally, fruits and vegetables supply dietary fiber, and fiber intake is linked to lower incidence of cardiovascular disease and obesity. Fruits and vegetables also supply vitamins and minerals to the diet and are sources of phytochemicals that function as antioxidants, phytoestrogens, and antiinflammatory agents and through other protective mechanisms. In this review, we describe the existing dietary guidance on intake of fruits and vegetables. We also review attempts to characterize fruits and vegetables into groups based on similar chemical structures and functions. Differences among fruits and vegetables in nutrient composition are detailed. We summarize the epidemiological and clinical studies on the health benefits of fruits and vegetables. Finally, we discuss the role of fiber in fruits and vegetables in disease prevention. PMID:22797986

Fruits and vegetables (image)

Science.gov (United States)

A healthy diet includes adding vegetables and fruit every day. Vegetables like broccoli, green beans, leafy greens, zucchini, cauliflower, cabbage, carrots, and tomatoes are low in calories and high in fiber, vitamins, and minerals. ...

Relishes: The new pickled vegetables

Directory of Open Access Journals (Sweden)

Tepić Aleksandra N.

2006-01-01

Full Text Available There have been an increasing interest of consumers for a ide variety of pickled vegetable products worldwide. Regarding the regional vegetable supplies and relatively poor assortment of ready-to-use products, the need to broaden the offer of domestic pickled vegetables at the market came out. In this work recipes for different vegetables, spices and condiments were developed. The best graded samples were analyzed for their main chemical composition (dry matter, proteins, oils and fats, total acidity, total sugars, sucrose, starch, cellulose, pH and energy- values.

Analysis of Post-Fire Vegetation Recovery in the Mediterranean Basin using MODIS Derived Vegetation Indices

Science.gov (United States)

Hawtree, Daniel; San Miguel, Jesus; Sedano, Fernando; Kempeneers, Pieter

2010-05-01

The Mediterranean basin region is highly susceptible to wildfire, with approximately 60,000 individual fires and half a million ha of natural vegetation burnt per year. Of particular concern in this region is the impact of repeated wildfires on the ability of natural lands to return to a pre-fire state, and of the possibility of desertification of semi-arid areas. Given these concerns, understanding the temporal patterns of vegetation recovery is important for the management of environmental resources in the region. A valuable tool for evaluating these recovery patterns are vegetation indices derived from remote sensing data. Previous research on post-fire vegetation recovery conducted in this region has found significant variability in recovery times across different study sites. It is unclear what the primary variables are affecting the differences in the rates of recovery, and if any geographic patterns of behavior exist across the Mediterranean basin. This research has primarily been conducted using indices derived from Landsat imagery. However, no extensive analysis of vegetation regeneration for large regions has been published, and assessment of vegetation recovery on the basis of medium-spatial resolution imagery such as that of MODIS has not yet been analyzed. This study examines the temporal pattern of vegetation recovery in a number of fire sites in the Mediterranean basin, using data derived from MODIS 16 -day composite vegetation indices. The intent is to develop a more complete picture of the temporal sequence of vegetation recovery, and to evaluate what additional factors impact variations in the recovery sequence. In addition, this study evaluates the utility of using MODIS derived vegetation indices for regeneration studies, and compares the findings to earlier studies which rely on Landsat data. Wildfires occurring between the years 2000 and 2004 were considered as potential study sites for this research. Using the EFFIS dataset, all wildfires

ERECTA, salicylic acid, abscisic acid, and jasmonic acid modulate quantitative disease resistance of Arabidopsis thaliana to Verticillium longisporum.

Science.gov (United States)

Häffner, Eva; Karlovsky, Petr; Splivallo, Richard; Traczewska, Anna; Diederichsen, Elke

2014-04-01

Verticillium longisporum is a soil-borne vascular pathogen infecting cruciferous hosts such as oilseed rape. Quantitative disease resistance (QDR) is the major control means, but its molecular basis is poorly understood so far. Quantitative trait locus (QTL) mapping was performed using a new (Bur×Ler) recombinant inbred line (RIL) population of Arabidopsis thaliana. Phytohormone measurements and analyses in defined mutants and near-isogenic lines (NILs) were used to identify genes and signalling pathways that underlie different resistance QTL. QTL for resistance to V. longisporum-induced stunting, systemic colonization by the fungus and for V. longisporum-induced chlorosis were identified. Stunting resistance QTL were contributed by both parents. The strongest stunting resistance QTL was shown to be identical with Erecta. A functional Erecta pathway, which was present in Bur, conferred partial resistance to V. longisporum-induced stunting. Bur showed severe stunting susceptibility in winter. Three stunting resistance QTL of Ler origin, two co-localising with wall-associated kinase-like (Wakl)-genes, were detected in winter. Furthermore, Bur showed a much stronger induction of salicylic acid (SA) by V. longisporum than Ler. Systemic colonization was controlled independently of stunting. The vec1 QTL on chromosome 2 had the strongest effect on systemic colonization. The same chromosomal region controlled the level of abscisic acid (ABA) and jasmonic acid (JA) in response to V. longisporum: The level of ABA was higher in colonization-susceptible Ler than in colonization-resistant Bur after V. longisporum infection. JA was down-regulated in Bur after infection, but not in Ler. These differences were also demonstrated in NILs, varying only in the region containing vec1. All phytohormone responses were shown to be independent of Erecta. Signalling systems with a hitherto unknown role in the QDR of A. thaliana against V. longisporum were identified: Erecta mediated

Predicting gender differences in liking for vegetables and preference for a variety of vegetables among 11-year-old children.

Science.gov (United States)

Lehto, Elviira; Ray, Carola; Haukkala, Ari; Yngve, Agneta; Thorsdottir, Inga; Roos, Eva

2015-12-01

We studied the factors that predict liking for vegetables and preference for a variety of vegetables among schoolchildren. Additionally, we examined if there were gender differences in the predictors that explain the hypothesized higher scores in liking vegetables and preferences among girls. The data from the PRO GREENS project included 424 Finnish children (response rate 77%) aged 11 to 12. The children completed validated measures about social and environmental factors related to their liking for vegetables and preferences both at baseline 2009 and follow-up 2010. The associations were examined with regression and mediation analyses. The strongest predictors of both girls' and boys' liking and preferences were higher levels of eating vegetables together with the family, previous vegetable intake and a lower level of perceived barriers. Liking was additionally predicted by a lower level of parental demand that their child should eat vegetables. Girls reported higher levels of liking and preferences in the follow-up. This gender difference was mainly explained by girls' lower level of perceived barriers related to vegetable intake and girls' higher previous vegetable intake. Interventions that aim to increase the low vegetable intake among boys by increasing their liking for vegetables and preference for a variety of vegetables could benefit from targeting perceived barriers, namely boys' perception and values concerning the consumption of vegetables. Copyright © 2015 Elsevier Ltd. All rights reserved.

Consumer attitudes towards vegetable attributes: potential buyers of pesticide-free vegetables in Accra and Kumasi, Ghana.

Science.gov (United States)

Probst, Lorenz; Aigelsperger, Lisa; Hauser, Michael

2010-01-01

Considering the inappropriate use of synthetic pesticides on vegetables in West Africa, the rationale behind this research was to assess the extent to which consumers can function as demanders of risk reduced vegetables and hence act as innovators towards vegetable safety. Using the cases of Kumasi and Accra in Ghana, the study examined possible consumer responses to product certification that communicates freedom from pesticides (e.g., organic certification). Generally, search attributes such as the fresh and healthy appearance of a vegetable were found to be central to consumer choice. While consumers stress the importance of health value, they are mostly unaware of agro-chemical risks related to vegetable consumption.

Microscale vegetation-soil feedback boosts hysteresis in a regional vegetation-climate system

NARCIS (Netherlands)

Janssen, R.H.H.; Meinders, M.B.J.; Nes, van E.H.; Scheffer, M.

2008-01-01

It has been hypothesized that a positive feedback between vegetation cover and monsoon circulation may lead to the existence of two alternative stable states in the Sahara region: a vegetated state with moderate precipitation and a desert state with low precipitation. This could explain the sudden

Vegetation Description, Rare Plant Inventory, and Vegetation Monitoring for Craig Mountain, Idaho.

Energy Technology Data Exchange (ETDEWEB)

Mancuso, Michael; Moseley, Robert

1994-12-01

The Craig Mountain Wildlife Mitigation Area was purchased by Bonneville Power Administration (BPA) as partial mitigation for wildlife losses incurred with the inundation of Dworshak Reservoir on the North Fork Clearwater River. Upon completion of the National Environmental Protection Act (NEPA) process, it is proposed that title to mitigation lands will be given to the Idaho Department of Fish and Game (IDFG). Craig Mountain is located at the northern end of the Hells Canyon Ecosystem. It encompasses the plateau and steep canyon slopes extending from the confluence of the Snake and Salmon rivers, northward to near Waha, south of Lewiston, Idaho. The forested summit of Craig Mountain is characterized by gently rolling terrain. The highlands dramatically break into the canyons of the Snake and Salmon rivers at approximately the 4,700 foot contour. The highly dissected canyons are dominated by grassland slopes containing a mosaic of shrubfield, riparian, and woodland habitats. During the 1993 and 1994 field seasons, wildlife, habitat/vegetation, timber, and other resources were systematically inventoried at Craig Mountain to provide Fish and Game managers with information needed to draft an ecologically-based management plan. The results of the habitat/vegetation portion of the inventory are contained in this report. The responsibilities for the Craig Mountain project included: (1) vegetation data collection, and vegetation classification, to help produce a GIS-generated Craig Mountain vegetation map, (2) to determine the distribution and abundance of rare plants populations and make recommendations concerning their management, and (3) to establish a vegetation monitoring program to evaluate the effects of Fish and Game management actions, and to assess progress towards meeting habitat mitigation goals.

Vegetation description, rare plant inventory, and vegetation monitoring for Craig Mountain, Idaho

International Nuclear Information System (INIS)

Mancuso, M.; Moseley, R.

1994-12-01

The Craig Mountain Wildlife Mitigation Area was purchased by Bonneville Power Administration (BPA) as partial mitigation for wildlife losses incurred with the inundation of Dworshak Reservoir on the North Fork Clearwater River. Upon completion of the National Environmental Protection Act (NEPA) process, it is proposed that title to mitigation lands will be given to the Idaho Department of Fish and Game (IDFG). Craig Mountain is located at the northern end of the Hells Canyon Ecosystem. It encompasses the plateau and steep canyon slopes extending from the confluence of the Snake and Salmon rivers, northward to near Waha, south of Lewiston, Idaho. The forested summit of Craig Mountain is characterized by gently rolling terrain. The highlands dramatically break into the canyons of the Snake and Salmon rivers at approximately the 4,700 foot contour. The highly dissected canyons are dominated by grassland slopes containing a mosaic of shrubfield, riparian, and woodland habitats. During the 1993 and 1994 field seasons, wildlife, habitat/vegetation, timber, and other resources were systematically inventoried at Craig Mountain to provide Fish and Game managers with information needed to draft an ecologically-based management plan. The results of the habitat/vegetation portion of the inventory are contained in this report. The responsibilities for the Craig Mountain project included: (1) vegetation data collection, and vegetation classification, to help produce a GIS-generated Craig Mountain vegetation map, (2) to determine the distribution and abundance of rare plants populations and make recommendations concerning their management, and (3) to establish a vegetation monitoring program to evaluate the effects of Fish and Game management actions, and to assess progress towards meeting habitat mitigation goals

Consumer Acceptance Comparison Between Seasoned and Unseasoned Vegetables.

Science.gov (United States)

Feng, Yiming; Albiol Tapia, Marta; Okada, Kyle; Castaneda Lazo, Nuria Blanca; Chapman-Novakofski, Karen; Phillips, Carter; Lee, Soo-Yeun

2018-02-01

Recent findings show that approximately 87% of the U.S. population fail to meet the vegetable intake recommendations, with unpleasant taste of vegetables being listed as the primary reason for this shortfall. In this study, spice and herb seasoning was used to enhance palatability of vegetables, in order to increase consumer acceptance. In total, 749 panelists were screened and recruited as specific vegetable likers of the vegetable being tested or general vegetable likers. Four sessions were designed to evaluate the effect of seasoning within each type of vegetable, including broccoli, cauliflower, carrot, and green bean. Each panelist was only allowed to participate in one test session to evaluate only one vegetable type, so as to mitigate potential learning effect. Overall, the results showed that seasoned vegetables were significantly preferred over unseasoned vegetables (P trend of seasoned vegetable being preferred remained. The findings from this study demonstrate the effect of seasoning in enhancing consumer liking of vegetables, which may lead to increased consumption to be assessed in future studies. To improve the sensory properties of vegetables, masking the bitter taste of vegetables using spice and herb seasoning are gaining increasing attention. Our findings suggest that the overall liking of vegetables could be improved by incorporating spice and herb seasonings that are specifically formulated for each vegetable. Ultimately, developing and commercializing spice and herb seasonings may aid to increase vegetable consumption, as well as expanding the vegetable seasoning market. © 2018 The Authors Journal of Food Science published by Wiley Periodicals, Inc. on behalf of Institute of Food Technologists.

[Differences of vegetation phenology monitoring by remote sensing based on different spectral vegetation indices.

Science.gov (United States)

Zuo, Lu; Wang, Huan Jiong; Liu, Rong Gao; Liu, Yang; Shang, Rong

2018-02-01

Vegetation phenology is a comprehensive indictor for the responses of terrestrial ecosystem to climatic and environmental changes. Remote sensing spectrum has been widely used in the extraction of vegetation phenology information. However, there are many differences between phenology extracted by remote sensing and site observations, with their physical meaning remaining unclear. We selected one tile of MODIS data in northeastern China (2000-2014) to examine the SOS and EOS differences derived from the normalized difference vegetation index (NDVI) and the simple ratio vegetation index (SR) based on both the red and near-infrared bands. The results showed that there were significant differences between NDVI-phenology and SR-phenology. SOS derived from NDVI averaged 18.9 days earlier than that from SR. EOS derived from NDVI averaged 19.0 days later than from SR. NDVI-phenology had a longer growing season. There were significant differences in the inter-annual variation of phenology from NDVI and SR. More than 20% of the pixel SOS and EOS derived from NDVI and SR showed the opposite temporal trend. These results caused by the seasonal curve characteristics and noise resistance differences of NDVI and SR. The observed data source of NDVI and SR were completely consistent, only the mathematical expressions were different, but phenology results were significantly different. Our results indicated that vegetation phenology monitoring by remote sensing is highly dependent on the mathematical expression of vegetation index. How to establish a reliable method for extracting vegetation phenology by remote sensing needs further research.

Handling Procedures of Vegetable Crops

Science.gov (United States)

Perchonok, Michele; French, Stephen J.

2004-01-01

The National Aeronautics and Space Administration (NASA) is working towards future long duration manned space flights beyond low earth orbit. The duration of these missions may be as long as 2.5 years and will likely include a stay on a lunar or planetary surface. The primary goal of the Advanced Food System in these long duration exploratory missions is to provide the crew with a palatable, nutritious, and safe food system while minimizing volume, mass, and waste. Vegetable crops can provide the crew with added nutrition and variety. These crops do not require any cooking or food processing prior to consumption. The vegetable crops, unlike prepackaged foods, will provide bright colors, textures (crispy), and fresh aromas. Ten vegetable crops have been identified for possible use in long duration missions. They are lettuce, spinach, carrot, tomato, green onion, radish, bell pepper, strawberries, fresh herbs, and cabbage. Whether these crops are grown on a transit vehicle (e.g., International Space Station) or on the lunar or planetary surface, it will be necessary to determine how to safely handle the vegetables while maintaining acceptability. Since hydrogen peroxide degrades into water and oxygen and is generally recognized as safe (GRAS), hydrogen peroxide has been recommended as the sanitizer. The objective of th is research is to determine the required effective concentration of hydrogen peroxide. In addition, it will be determined whether the use of hydrogen peroxide, although a viable sanitizer, adversely affects the quality of the vegetables. Vegetables will be dipped in 1 % hydrogen peroxide, 3% hydrogen peroxide, or 5% hydrogen peroxide. Treated produce and controls will be stored in plastic bags at 5 C for up to 14 days. Sensory, color, texture, and total plate count will be measured. The effect on several vegetables including lettuce, radish, tomato and strawberries has been completed. Although each vegetable reacts to hydrogen peroxide differently, the

The use of a commercial vegetable juice as a practical means to increase vegetable intake: a randomized controlled trial.

Science.gov (United States)

Shenoy, Sonia F; Kazaks, Alexandra G; Holt, Roberta R; Chen, Hsin Ju; Winters, Barbara L; Khoo, Chor San; Poston, Walker S C; Haddock, C Keith; Reeves, Rebecca S; Foreyt, John P; Gershwin, M Eric; Keen, Carl L

2010-09-17

Recommendations for daily dietary vegetable intake were increased in the 2005 USDA Dietary Guidelines as consumption of a diet rich in vegetables has been associated with lower risk of certain chronic health disorders including cardiovascular disease. However, vegetable consumption in the United States has declined over the past decade; consequently, the gap between dietary recommendations and vegetable intake is widening. The primary aim of this study is to determine if drinking vegetable juice is a practical way to help meet daily dietary recommendations for vegetable intake consistent with the 2005 Dietary Guidelines and the Dietary Approaches to Stop Hypertension (DASH) diet. The secondary aim is to assess the effect of a vegetable juice on measures of cardiovascular health. We conducted a 12-week, randomized, controlled, parallel-arm study consisting of 3 groups of free-living, healthy volunteers who participated in study visits at the Ragle Human Nutrition Research Center at the University of California, Davis. All subjects received education on the DASH diet and 0, 8 or 16 fluid ounces of vegetable juice daily. Assessments were completed of daily vegetable servings before and after incorporation of vegetable juice and cardiovascular health parameters including blood pressure. Without the juice, vegetable intake in all groups was lower than the 2005 Dietary Guidelines and DASH diet recommendations. The consumption of the vegetable juice helped participants reach recommended intake. In general, parameters associated with cardiovascular health did not change over time. However, in the vegetable juice intervention groups, subjects who were pre-hypertensive at the start of the study showed a significant decrease in blood pressure during the 12-week intervention period. Including 1-2 cups of vegetable juice daily was an effective and acceptable way for healthy adults to close the dietary vegetable gap. Increase in daily vegetable intake was associated with a

The use of a commercial vegetable juice as a practical means to increase vegetable intake: a randomized controlled trial

Directory of Open Access Journals (Sweden)

Reeves Rebecca S

2010-09-01

Full Text Available Abstract Background Recommendations for daily dietary vegetable intake were increased in the 2005 USDA Dietary Guidelines as consumption of a diet rich in vegetables has been associated with lower risk of certain chronic health disorders including cardiovascular disease. However, vegetable consumption in the United States has declined over the past decade; consequently, the gap between dietary recommendations and vegetable intake is widening. The primary aim of this study is to determine if drinking vegetable juice is a practical way to help meet daily dietary recommendations for vegetable intake consistent with the 2005 Dietary Guidelines and the Dietary Approaches to Stop Hypertension (DASH diet. The secondary aim is to assess the effect of a vegetable juice on measures of cardiovascular health. Methods We conducted a 12-week, randomized, controlled, parallel-arm study consisting of 3 groups of free-living, healthy volunteers who participated in study visits at the Ragle Human Nutrition Research Center at the University of California, Davis. All subjects received education on the DASH diet and 0, 8 or 16 fluid ounces of vegetable juice daily. Assessments were completed of daily vegetable servings before and after incorporation of vegetable juice and cardiovascular health parameters including blood pressure. Results Without the juice, vegetable intake in all groups was lower than the 2005 Dietary Guidelines and DASH diet recommendations. The consumption of the vegetable juice helped participants reach recommended intake. In general, parameters associated with cardiovascular health did not change over time. However, in the vegetable juice intervention groups, subjects who were pre-hypertensive at the start of the study showed a significant decrease in blood pressure during the 12-week intervention period. Conclusion Including 1-2 cups of vegetable juice daily was an effective and acceptable way for healthy adults to close the dietary vegetable

[Correlation analysis on normalized difference vegetation index (NDVI) of different vegetations and climatic factors in Southwest China].

Science.gov (United States)

Zhang, Yuan-Dong; Zhang, Xiao-He; Liu, Shi-Rong

2011-02-01

Based on the 1982-2006 NDVI remote sensing data and meteorological data of Southwest China, and by using GIS technology, this paper interpolated and extracted the mean annual temperature, annual precipitation, and drought index in the region, and analyzed the correlations of the annual variation of NDVI in different vegetation types (marsh, shrub, bush, grassland, meadow, coniferous forest, broad-leaved forest, alpine vegetation, and cultural vegetation) with corresponding climatic factors. In 1982-2006, the NDVI, mean annual temperature, and annual precipitation had an overall increasing trend, and the drought index decreased. Particularly, the upward trend of mean annual temperature was statistically significant. Among the nine vegetation types, the NDVI of bush and mash decreased, and the downward trend was significant for bush. The NDVI of the other seven vegetation types increased, and the upward trend was significant for coniferous forest, meadow, and alpine vegetation, and extremely significant for shrub. The mean annual temperature in the areas with all the nine vegetation types increased significantly, while the annual precipitation had no significant change. The drought index in the areas with marsh, bush, and cultural vegetation presented an increasing trend, that in the areas with meadow and alpine vegetation decreased significantly, and this index in the areas with other four vegetation types had an unobvious decreasing trend. The NDVI of shrub and coniferous forest had a significantly positive correlation with mean annual temperature, and that of shrub and meadow had significantly negative correlation with drought index. Under the conditions of the other two climatic factors unchanged, the NDVI of coniferous forest, broad-leaved forest, and alpine vegetation showed the strongest correlation with mean annual temperature, that of grass showed the strongest correlation with annual precipitation, and the NDVI of mash, shrub, grass, meadow, and cultural

Effects of metals on vegetation

Energy Technology Data Exchange (ETDEWEB)

Press, N P

1972-01-01

Prospectors have long known that abnormal concentrations of metals in the soil overlying an ore deposit can affect the vegetation rooting in this soil; mineral deposits have even been discovered because of such vegetation changes. Recently, many people have become interested in the possibility of remote sensing such vegetation changes, and perhaps using the results in conjunction with airborne geophysics and photogeological interpretation in integrated prospecting programs.

Vegetation and acidification, Chapter 5

Science.gov (United States)

David R. DeWalle; James N. Kochenderfer; Mary Beth Adams; Gary W. Miller

2006-01-01

In this chapter, the impact of watershed acidification treatments on WS3 at the Fernow Experimental Forest (FEF) and at WS9 on vegetation is presented and summarized in a comprehensive way for the first time. WS7 is used as a vegetative reference basin for WS3, while untreated plots within WS9 are used as a vegetative reference for WS9. Bioindicators of acidification...

Pairing vegetables with a liked food and visually appealing presentation: promising strategies for increasing vegetable consumption among preschoolers.

Science.gov (United States)

Correia, Danielle C S; O'Connell, Meghan; Irwin, Melinda L; Henderson, Kathryn E

2014-02-01

Vegetable consumption among preschool children is below recommended levels. New evidence-based approaches to increase preschoolers' vegetable intake, particularly in the child care setting, are needed. This study tests the effectiveness of two community-based randomized interventions to increase vegetable consumption and willingness to try vegetables: (1) the pairing of a vegetable with a familiar, well-liked food and (2) enhancing the visual appeal of a vegetable. Fifty-seven preschoolers enrolled in a Child and Adult Care Food Program-participating child care center participated in the study; complete lunch and snack data were collected from 43 and 42 children, respectively. A within-subjects, randomized design was used, with order of condition counterbalanced. For lunch, steamed broccoli was served either on the side of or on top of cheese pizza. For a snack, raw cucumber was served either as semicircles with chive and an olive garnish or arranged in a visually appealing manner (in the shape of a caterpillar). Paired t-tests were used to determine differences in consumption of meal components, and McNemar's test was performed to compare willingness to taste. Neither visual appeal enhancement nor pairing with a liked food increased vegetable consumption. Pairing increased willingness to try the vegetable from 79% to 95% of children (p=0.07). Greater vegetable intake occurred at snack than at lunch. Further research should explore the strategy of pairing vegetables with liked foods. Greater consumption at snack underscores snack time as a critical opportunity for increasing preschool children's vegetable intake.

Toxicología Vegetal

OpenAIRE

García Fernández, Antonio Juan

2010-01-01

Presentaciones de clase de los temas de Toxicología Vegetal de la licenciatura de Veterinaria de la Universidad de Murcia del curso 2011/12. Presentaciones de Toxicología Vegetal de la asignatura de Toxicología de la Licenciatura de Veterinaria del curso 2011/12

Variation of strontium stable isotope ratios and origins of strontium in Japanese vegetables and comparison with Chinese vegetables.

Science.gov (United States)

Aoyama, Keisuke; Nakano, Takanori; Shin, Ki-Cheol; Izawa, Atsunobu; Morita, Sakie

2017-12-15

To evaluate the utility of 87 Sr/ 86 Sr ratio for determining the geographical provenance of vegetables, we compared 87 Sr/ 86 Sr ratios and Sr concentrations in five vegetable species grown in Japan and China, and we also examined the relationships between 87 Sr/ 86 Sr ratios in vegetables, the soil-exchangeable pool, irrigation water, and fertilizer from 20 Japanese agricultural areas. The vegetable 87 Sr/ 86 Sr ratios in Japan were similar for all species within a given agricultural area, but tended to be low in northeast Japan and high in southwest Japan. The median 87 Sr/ 86 Sr ratio in Japanese vegetables was similar to that in fertilizer, suggesting that in addition to rock-derived Sr, vegetables contain Sr derived from fertilizers. In most cases, the 87 Sr/ 86 Sr ratios for the Japanese and Chinese vegetables differed by approximately 0.710. Linear discriminant analysis using both 87 Sr/ 86 Sr and the Sr concentration allowed more accurate discrimination between vegetables from the two countries. Copyright © 2017 Elsevier Ltd. All rights reserved.

Vegetable communities and degradation patterns and succession in the vegetation of the Western Hills of Chia, Cundinamarca - Colombia

International Nuclear Information System (INIS)

Cortes S, Sandra P; Van der Hammen, Thomas; Rangel Ch, J Orlando

1999-01-01

Two physiognomic plant communities were characterized: open vegetation which include the associations Dichondro repentis - Cupheetum serpyllifoliae (meadows), Baccharido rupicolae - Dodonaeetum viscosae (low shrubby vegetation), Andropogono aequatoriensis - Epidendretum elongatae (casmophytic vegetation) and Chaetolepido microphyllae-Espeletiopsietum corymbosae (shrubby vegetation-type with rosette plants). The closed vegetation includes two kinds of forests, Miconio ligustrinae- Weinmannietum tomentosae and Daphnopsio caracasanae - Xylosmetum spiculiferae. According to the floristic composition, depth of the soil, slope climate, relief and the anthropic influence (including degradation and natural succession), two trends in plant succession were defined

Hiding vegetables to reduce energy density: an effective strategy to increase children's vegetable intake and reduce energy intake.

Science.gov (United States)

Spill, Maureen K; Birch, Leann L; Roe, Liane S; Rolls, Barbara J

2011-09-01

Strategies are needed to increase children's intake of a variety of vegetables, including vegetables that are not well liked. We investigated whether incorporating puréed vegetables into entrées to reduce the energy density (ED; in kcal/g) affected vegetable and energy intake over 1 d in preschool children. In this crossover study, 3- to 5-y-old children (n = 40) were served all meals and snacks 1 d/wk for 3 wk. Across conditions, entrées at breakfast, lunch, dinner, and evening snack were reduced in ED by increasing the proportion of puréed vegetables. The conditions were 100% ED (standard), 85% ED (tripled vegetable content), and 75% ED (quadrupled vegetable content). Entrées were served with unmanipulated side dishes and snacks, and children were instructed to eat as much as they liked. The daily vegetable intake increased significantly by 52 g (50%) in the 85% ED condition and by 73 g (73%) in the 75% ED condition compared with that in the standard condition (both P daily energy intake decreased by 142 kcal (12%) from the 100% to 75% ED conditions (P daily vegetable intake and decrease the energy intake in young children. This trial was registered at clinicaltrials.gov as NCT01252433.

Vegetation of the Hantam-Tanqua-Roggeveld subregion, South Africa Part 2: Succulent Karoo Biome related vegetation

Directory of Open Access Journals (Sweden)

Helga van der Merwe

2009-03-01

Full Text Available The Hantam-Tanqua-Roggeveld subregion lies within the Succulent Karoo Hotspot that stretches along the western side of the Republic of South Africa and Namibia. This project, carried out to document the botanical diversity in the Hantam-Tanqua-Roggeveld subregion, was part of a project identified as a priority during the SKEP (Succulent Karoo Ecosystem Programme initiative in this Hotspot. Botanical surveys were conducted in an area covering over three million hectares. Satellite images of the area and topocadastral, land type and geology maps were used to stratify the area into relatively homogeneous units. An analysis of the floristic data of 390 sample plots identified two major floristic units, i.e. the Fynbos Biome related vegetation and the Succulent Karoo Biome related vegetation. A description of the vegetation related to the Succulent Karoo Biome is presented in this article. Seven associations, 16 subassociations and several mosaic vegetation units, consisting of more than one vegetation unit, were identified and mapped. Various threats to the vegetation in the region were identified during the survey and are briefly discussed.

Vegetation and soils

Science.gov (United States)

Burke, M.K.; King, S.L.; Eisenbies, M.H.; Gartner, D.

2000-01-01

Intro paragraph: Characterization of bottomland hardwood vegetation in relatively undisturbed forests can provide critical information for developing effective wetland creation and restoration techniques and for assessing the impacts of management and development. Classification is a useful technique in characterizing vegetation because it summarizes complex data sets, assists in hypothesis generation about factors influencing community variation, and helps refine models of community structure. Hierarchical classification of communities is particularly useful for showing relationships among samples (Gauche 1982).

Phenological characteristics of the main vegetation types on the Tibetan Plateau based on vegetation and water indices

International Nuclear Information System (INIS)

Peng, D L; Huang, W J; Zhou, B; Li, C J; Wu, Y P; Yang, X H

2014-01-01

Plant phenology is considered one of the most sensitive and easily observable natural indicators of climate change, though few studies have focused on the heterogeneities of phenology across the different vegetation types. In this study, we tried to find the phenological characteristics of the main vegetation types on the Tibetan Plateau. MCD12Q1 images over the Tibetan Plateau from 2001 to 2010 were used to extract the main vegetation types. The Normalized Difference Vegetation Index (NDVI), Enhanced Vegetation Index (EVI), and Land Surface Water Index (LSWI) were calculated using surface reflectance values from the blue, red, near-infrared, short-wave infrared (SWIR) 6 (for LSIW6), and SWIR7 (for LSIW7) bands derived from MOD09A1 and used to explore the phenological characteristics of the main vegetation types on the Tibetan Plateau. The results showed that there were eight constant vegetation types on the Tibetan Plateau from 2001 to 2010 demonstrating multiple phenological characteristics. Evergreen needleleaf forest, evergreen broadleaf forest, and permanent wetland had the minimum NDVI values during the summer season, while open shrubland and grassland had the maximum NDVI/EVI values during this period. NDVI and EVI of cropland/natural vegetation had two peaks for their seasonal variations. EVI showed a more significant correlation with LSWI6/LSWI7 than NDVI. Compared to LSWI7, larger EVI values occurred in evergreen needleleaf forest, evergreen broadleaf forest, mixed forest, and permanent wetland, while smaller values occurred in shrubland and barren or sparsely vegetated cover, and nearly equal values occurred in grassland and cropland

Chapter 3: Status and trends of vegetation

Science.gov (United States)

James M. Guldin; Frank R. Thompson; Lynda L. Richards; Kyra C. Harper

1999-01-01

This chapter provides information about the vegetation cover of the Assessment area. The types and areal extent of vegetation in the Highlands are of interest for many reasons. Vegetation cover largely determines the availability of habitat for terrestrial animals, plants, and other organisms. Vegetation cover strongly influences what uses {e.g., timber, forage,...

Investigation on the Patterns of Global Vegetation Change Using a Satellite-Sensed Vegetation Index

Directory of Open Access Journals (Sweden)

Ainong Li

2010-06-01

Full Text Available The pattern of vegetation change in response to global change still remains a controversial issue. A Normalized Difference Vegetation Index (NDVI dataset compiled by the Global Inventory Modeling and Mapping Studies (GIMMS was used for analysis. For the period 1982–2006, GIMMS-NDVI analysis indicated that monthly NDVI changes show homogenous trends in middle and high latitude areas in the northern hemisphere and within, or near, the Tropic of Cancer and Capricorn; with obvious spatio-temporal heterogeneity on a global scale over the past two decades. The former areas featured increasing vegetation activity during growth seasons, and the latter areas experienced an even greater amplitude in places where precipitation is adequate. The discussion suggests that one should be cautious of using the NDVI time-series to analyze local vegetation dynamics because of its coarse resolution and uncertainties.

Accumulation and health risk of heavy metals in vegetables from harmless and organic vegetable production systems of China.

Science.gov (United States)

Chen, Yong; Hu, Wenyou; Huang, Biao; Weindorf, David C; Rajan, Nithya; Liu, Xiaoxiao; Niedermann, Silvana

2013-12-01

Heavy metal accumulation in vegetables is a growing concern for public health. Limited studies have elucidated the heavy metal accumulation characteristics and health risk of different vegetables produced in different facilities such as greenhouses and open-air fields and under different management modes such as harmless and organic. Given the concern over the aforementioned factors related to heavy metal accumulation, this study selected four typical greenhouse vegetable production bases, short-term harmless greenhouse vegetable base (SHGVB), middle-term harmless greenhouse vegetable base (MHGVB), long-term harmless greenhouse vegetable base (LHGVB), and organic greenhouse vegetable base (OGVB), in Nanjing City, China to study heavy metal accumulation in different vegetables and their associated health risks. Results showed that soils and vegetables from SHGVB and OGVB apparently accumulated fewer certain heavy metals than those from other bases, probably due to fewer planting years and special management, respectively. Greenhouse conditions significantly increased certain soil heavy metal concentrations relative to open-air conditions. However, greenhouse conditions did not significantly increase concentrations of As, Cd, Cu, Hg, and Zn in leaf vegetables. In fact, under greenhouse conditions, Pb accumulation was effectively reduced. The main source of soil heavy metals was the application of large amounts of low-grade fertilizer. There was larger health risk for producers' children to consume vegetables from the three harmless vegetable bases than those of residents' children. The hazard index (HI) over a large area exceeded 1 for these two kinds of children in the MHGVB and LHGVB. There was also a slight risk in the SHGVB for producers' children solely. However, the HI of the whole area of the OGVB for two kinds of children was below 1, suggesting low risk of heavy metal exposure through the food chain. Notably, the contribution rate of Cu and Zn to the HI were

Mutation breeding in vegetable crops

Energy Technology Data Exchange (ETDEWEB)

Yamaguchi, Takashi

1984-03-01

Vegetables breed by seeds and vegetative organs. In main vegetables, the differentiation of clopping types, the adoption of monoculture and year-round production and shipment are carried out, adapting to various socio-economic and cultivation conditions. Protected agriculture has advanced mainly for fruit vegetables, and the seeds for sale have become almost hybrid varieties. Reflecting this situation, the demand for breeding is diversified and characteristic. The present status of mutation breeding of vegetables is not yet well under way, but reports of about 40 raised varieties have been published in the world. The characters introduced by induced mutation and irradiation are compact form, harvesting aptitude, the forms and properties of stems and leaves, anti-lodging property, the size, form and uniformity of fruits, male sterility and so on. The radiation sources used were mostly gamma ray or X-ray, but sometimes, combined irradiation was used. Results obtained in Japan include: burdocks as an example to gamma ray irradiation of seeds; tomatoes as an example of inducing compound resistance against disease injury; and lettuce as an example of internal beta irradiation. (Kako, I.).

Review of Alternative Management Options of Vegetable Crop Residues to Reduce Nitrate Leaching in Intensive Vegetable Rotations

Directory of Open Access Journals (Sweden)

Laura Agneessens

2014-12-01

Full Text Available Vegetable crop residues take a particular position relative to arable crops due to often large amounts of biomass with a N content up to 200 kg N ha−1 left behind on the field. An important amount of vegetable crops are harvested during late autumn and despite decreasing soil temperatures during autumn, high rates of N mineralization and nitrification still occur. Vegetable crop residues may lead to considerable N losses through leaching during winter and pose a threat to meeting water quality objectives. However, at the same time vegetable crop residues are a vital link in closing the nutrient and organic matter cycle of soils. Appropriate and sustainable management is needed to harness the full potential of vegetable crop residues. Two fundamentally different crop residue management strategies to reduce N losses during winter in intensive vegetable rotations are reviewed, namely (i on-field management options and modifications to crop rotations and (ii removal of crop residues, followed by a useful and profitable application.

CONSIDERATIONS ON ROMANIA’S VEGETABLE MARKET

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Agatha POPESCU

2013-12-01

Full Text Available The paper aimed to present the situation of Romania’s vegetable market in the period 2007-2011 based on the statistical data regarding the main vegetables: tomatoes, onion, garlic, cabbage, green peppers and melons. The vegetable production increased by 33.99 from 3,166.8 tons in 2007 to 4,176.3 tons in 2011.This was due to the yield gain as follows: 58.55 % for melons, 27.62 % for green peppers, 27.05 % for tomatoes, 25.99 % for dry garlic, 24.96 % for dry onion, 12.61 % for white cabbage. In 2011, the contribution of various categories of vegetables to production was: 24.55 % white cabbage, 21.81 % tomatoes, 15.45 % melons, 9.44 % onion, 6.06 % green pepper, 1.59 % garlic and 21.1 % other vegetables. The contribution of the micro regions to vegetable production in 2011 was: 19.46 % South Muntenia, 18.95 % South East Romania, 17.30 % South West Oltenia, 15.92 % North East Romania, 10.43 % West Romania, 8.47 % North West Romania, 6.54 % Central Romania, 2.93 % Bucharest Ilfov. Vegetable production per inhabitant is higher in Romania compared to the average production per capita in the EU. The average consumption increased as a postive aspect reflecting the obtained production and import. Vegetable production should increase in order to cover much better the doestic market needs and support export to the EU market.

Wetland vegetation establishment in L-Lake

International Nuclear Information System (INIS)

Kroeger, S.R.

1990-07-01

Wetland vegetation was transplanted from PAR Pond to L-Lake between January and August, 1987. Approximately 100,000 individual plants representing over 40 species were transplanted along the southern shoreline. Three zones of vegetation were created: (1) submersed/floating-leaved, (2) emergent, (3) upper emergent/shrub. During the summers of 1987, 1988, 1989, the Savannah River Ecology Laboratory sampled the vegetation in 54 permanent transects located in planted (N=32) and unplanted areas (N=22). The 1989 vegetation data from L-Lake were compared to 1985 data from PAR Pond

Cruciferous Vegetables and Cancer Prevention

Science.gov (United States)

... case-control studies have found that people who ate greater amounts of cruciferous vegetables had a lower ... Professionals’ Follow-up Study—showed that women who ate more than 5 servings of cruciferous vegetables per ...

componente vegetal

Directory of Open Access Journals (Sweden)

Fabio Moscovich

2005-01-01

Full Text Available In order to determine environmental impact, indicators based on vegetation characteristics that would generate the forestry monoculture with the adjacent native forest, 32 sample unit were installed in an area of LIPSIA private enterprise, Esperanza Department, Misiones with those characteristics. The plots of 100 m2 were distributed systematically every 25 meters. The vegetation was divided in stratum: superior (DBH ≥ 10 cm, middle (1,6 cm ≤ DBH > 10 cm and inferior (DBH< cm. There were installed 10 plots in a logged native forest, 10 plots in a 18 years old Pinus elliottii Engelm. with approximately 400 trees/ha., 6 plots in a 10 – 25 years old Araucaria angustifolia (Bertd. Kuntze limiting area with approximately 900 trees/ha., and 6 plots located in this plantation. In the studied area were identified 150 vegetation species. In the inferior stratum there were found differences as function of various floristic diversity indexes. In all the cases the native forest showed larger diversity than plantations, followed by Pinus elliottii, Araucaria plantation and Araucaria limiting area. All the studied forest fitted to a logarithmical series of species distributions, that would indicate the incidence of a environmental factor in this distribution.

Soil-vegetation-atmosphere transfer modeling

Energy Technology Data Exchange (ETDEWEB)

Ikonen, J P; Sucksdorff, Y [Finnish Environment Agency, Helsinki (Finland)

1997-12-31

In this study the soil/vegetation/atmosphere-model based on the formulation of Deardorff was refined to hour basis and applied to a field in Vihti. The effect of model parameters on model results (energy fluxes, temperatures) was also studied as well as the effect of atmospheric conditions. The estimation of atmospheric conditions on the soil-vegetation system as well as an estimation of the effect of vegetation parameters on the atmospheric climate was estimated. Areal surface fluxes, temperatures and moistures were also modelled for some river basins in southern Finland. Land-use and soil parameterisation was developed to include properties and yearly variation of all vegetation and soil types. One classification was selected to describe the hydrothermal properties of the soils. Evapotranspiration was verified against the water balance method

Soil-vegetation-atmosphere transfer modeling

Energy Technology Data Exchange (ETDEWEB)

Ikonen, J.P.; Sucksdorff, Y. [Finnish Environment Agency, Helsinki (Finland)

1996-12-31

In this study the soil/vegetation/atmosphere-model based on the formulation of Deardorff was refined to hour basis and applied to a field in Vihti. The effect of model parameters on model results (energy fluxes, temperatures) was also studied as well as the effect of atmospheric conditions. The estimation of atmospheric conditions on the soil-vegetation system as well as an estimation of the effect of vegetation parameters on the atmospheric climate was estimated. Areal surface fluxes, temperatures and moistures were also modelled for some river basins in southern Finland. Land-use and soil parameterisation was developed to include properties and yearly variation of all vegetation and soil types. One classification was selected to describe the hydrothermal properties of the soils. Evapotranspiration was verified against the water balance method

Use of Different Vegetable Products to Increase Preschool-Aged Children's Preference for and Intake of a Target Vegetable

NARCIS (Netherlands)

Wild, de Victoire W.T.; Graaf, de Kees; Jager, Gerry

2017-01-01

Background: Children's low vegetable consumption requires effective strategies to enhance preference for and intake of vegetables. Objective: The study compared three preparation practices for a target vegetable (spinach) on their effectiveness in increasing preschool-aged children's preference

Radiocesium cycling in vegetation and soil

International Nuclear Information System (INIS)

Dahlman, R.C.; Francis, C.W.; Tamura, T.

1975-01-01

Data on cesium dynamics in vegetation and soils are reviewed. Special emphasis is placed on typical environments in the southeastern United States. Clay minerals of soil, especially micaceous types, effectively fix cesium and remove it from biotic components of ecosystems. Fallout 137 Cs enters food chains principally by direct deposition on vegetation; uptake by the root pathway is usually less than direct contamination of plant foliage. Cesium-137 levels in vegetation of the southeastern U. S. Coastal Plain are estimated on the basis of direct deposition from the atmosphere and root uptake from soil. Estimated concentrations, based on current concepts of 137 Cs dynamics in vegetation and fixation in soil, are in good agreement with observed values of 137 Cs in vegetation collected in 1969 and 1970. Mechanisms of direct deposition and of increased uptake by roots due to the absence of micaceous clays adequately explain the higher levels of 137 Cs in vegetation of the Coastal Plain and thus are responsible for the elevated 137 Cs reported for milk of the Tampa, Fla., milkshed

[Effects of road construction on regional vegetation types].

Science.gov (United States)

Liu, Shi-Liang; Liu, Qi; Wang, Cong; Yang, Jue-Jie; Deng, Li

2013-05-01

As a regional artificial disturbance component, road exerts great effects on vegetation types, and plays a substantial role in defining vegetation distribution to a certain extent. Aiming at the tropical rainforest degradation and artificial forest expansion in Yunnan Province of Southwest China, this paper analyzed the effects of road network extension on regional vegetation types. In the Province, different classes of roads had different effects on the vegetation types, but no obvious regularity was observed in the effects on the patch areas of different vegetation types due to the great variations of road length and affected distance. However, the vegetation patch number was more affected by lower class roads because of their wide distribution. As for different vegetation types, the vegetations on cultivated land were most affected by roads, followed by Castanopsis hystrix and Schima wallichii forests. Road network formation contributed most to the vegetation fragmentation, and there existed significant correlations between the human disturbance factors including village- and road distributions.

The marsh vegetation of Kleinmond Lagoon

Directory of Open Access Journals (Sweden)

M. O'Callaghan

1994-10-01

Full Text Available The vegetation of Kleinmond Lagoon suggests that this system is in transition from an estuary to a coastal lake. Two major types of vegetation were recognized, one which is subjected to soil and water conditions of marine origin and the other which is subjected to conditions of terrestrial origin. These vegetation types are discussed and compared to the vegetation of other estuarine systems. Artificial manipulations of the mouth seem to have resulted in sediment deposition and a freshening of the system. These unseasonable manipulations also threaten the continued existence of a number of species in the system.

Vegetable Grafting: The Implications of a Growing Agronomic Imperative for Vegetable Fruit Quality and Nutritive Value

Directory of Open Access Journals (Sweden)

Marios C. Kyriacou

2017-05-01

Full Text Available Grafting has become an imperative for intensive vegetable production since chlorofluorocarbon-based soil fumigants were banned from use on grounds of environmental protection. Compelled by this development, research into rootstock–scion interaction has broadened the potential applications of grafting in the vegetable industry beyond aspects of soil phytopathology. Grafting has been increasingly tapped for cultivation under adverse environs posing abiotic and biotic stresses to vegetable crops, thus enabling expansion of commercial production onto otherwise under-exploited land. Vigorous rootstocks have been employed not only in the open field but also under protected cultivation where increase in productivity improves distribution of infrastructural and energy costs. Applications of grafting have expanded mainly in two families: the Cucurbitaceae and the Solanaceae, both of which comprise major vegetable crops. As the main drives behind the expansion of vegetable grafting have been the resistance to soilborne pathogens, tolerance to abiotic stresses and increase in yields, rootstock selection and breeding have accordingly conformed to the prevailing demand for improving productivity, arguably at the expense of fruit quality. It is, however, compelling to assess the qualitative implications of this growing agronomic practice for human nutrition. Problems of impaired vegetable fruit quality have not infrequently been associated with the practice of grafting. Accordingly, the aim of the current review is to reassess how the practice of grafting and the prevalence of particular types of commercial rootstocks influence vegetable fruit quality and, partly, storability. Physical, sensorial and bioactive aspects of quality are examined with respect to grafting for watermelon, melon, cucumber, tomato, eggplant, and pepper. The physiological mechanisms at play which mediate rootstock effects on scion performance are discussed in interpreting the

Soil and vegetation surveillance

Energy Technology Data Exchange (ETDEWEB)

Antonio, E.J.

1995-06-01

Soil sampling and analysis evaluates long-term contamination trends and monitors environmental radionuclide inventories. This section of the 1994 Hanford Site Environmental Report summarizes the soil and vegetation surveillance programs which were conducted during 1994. Vegetation surveillance is conducted offsite to monitor atmospheric deposition of radioactive materials in areas not under cultivation and onsite at locations adjacent to potential sources of radioactivity.

Mutation breeding in vegetable crops

International Nuclear Information System (INIS)

Yamaguchi, Takashi

1984-01-01

Vegetables breed by seeds and vegetative organs. In main vegetables, the differentiation of clopping types, the adoption of monoculture and year-round production and shipment are carried out, adapting to various socio-economic and cultivation conditions. Protected agriculture has advanced mainly for fruit vegetables, and the seeds for sale have become almost hybrid varieties. Reflecting the situation like this, the demand for breeding is diversified and characteristic, and the case of applying mutation breeding seems to be many. The present status of the mutation breeding of vegetables is not yet well under way, but about 40 raised varieties have been published in the world. The characters introduced by induced mutation and irradiation were compact form, harvesting aptitude, the forms and properties of stems and leaves, anti-lodging property, the size, form and uniformity of fruits, male sterility and so on. The radiation sources used were mostly gamma ray or X-ray, but sometimes, combined irradiation was used. As the results obtained in Japan, burdocks as an example of gamma ray irradiation to seeds, tomatoes as an example of inducing the compound resistance against disease injury and lettuces as an example of internal beta irradiation are reported. (Kako, I.)

The Vegetables Turned:

DEFF Research Database (Denmark)

Carter, Dale

2009-01-01

in the relationship between creative artists and the Anglo-American popular music industry in the mid-1960s. Finally, and in retrospect, the figure of the vegetable cast into relief the counter-culture's utopian and dystopian dynamics as manifested in these song-writers' personal lives, now rendered as contemporary...... lyricist Van Dyke Parks, the incongruous, semantically complex figure of the vegetable came to illuminate aspects of psychedelic consciousness and - part by design, part by accident - the link between LSD and Anglo-American popular music. It threw light, too, on the scope and limits of changes...

Climatic drivers of vegetation based on wavelet analysis

Science.gov (United States)

Claessen, Jeroen; Martens, Brecht; Verhoest, Niko E. C.; Molini, Annalisa; Miralles, Diego

2017-04-01

Vegetation dynamics are driven by climate, and at the same time they play a key role in forcing the different bio-geochemical cycles. As climate change leads to an increase in frequency and intensity of hydro-meteorological extremes, vegetation is expected to respond to these changes, and subsequently feed back on their occurrence. This response can be analysed using time series of different vegetation diagnostics observed from space, in the optical (e.g. Normalised Difference Vegetation Index (NDVI), Solar Induced Fluorescence (SIF)) and microwave (Vegetation Optical Depth (VOD)) domains. In this contribution, we compare the climatic drivers of different vegetation diagnostics, based on a monthly global data-cube of 24 years at a 0.25° resolution. To do so, we calculate the wavelet coherence between each vegetation-related observation and observations of air temperature, precipitation and incoming radiation. The use of wavelet coherence allows unveiling the scale-by-scale response and sensitivity of the diverse vegetation indices to their climatic drivers. Our preliminary results show that the wavelet-based statistics prove to be a suitable tool for extracting information from different vegetation indices. Going beyond traditional methods based on linear correlations, the application of wavelet coherence provides information about: (a) the specific periods at which the correspondence between climate and vegetation dynamics is larger, (b) the frequencies at which this correspondence occurs (e.g. monthly or seasonal scales), and (c) the time lag in the response of vegetation to their climate drivers, and vice versa. As expected, areas of high rainfall volumes are characterised by a strong control of radiation and temperature over vegetation. Furthermore, precipitation is the most important driver of vegetation variability over short terms in most regions of the world - which can be explained by the rapid response of leaf development towards available water content

Kuchler Vegetation

Data.gov (United States)

California Natural Resource Agency — Digital version of potential natural plant communites as compiled and published on 'Map of the Natural Vegetation of California' by A. W. Kuchler, 1976. Source map...

SPINIFICI-SCAEVOLETEA SERICEAE, A NEW VEGETATION CLASS FOR PSAMMOPHYTIC DUNE VEGETATION IN THAILAND

Directory of Open Access Journals (Sweden)

S. PIGNATII

1996-04-01

Full Text Available This is a short account on the coastal dune vegetation of the Gulf of Siam in Thailand. Vegetation is mainly composed by succulent creeping plants with herbaceous habit as to Canavalia maritima (Papilionaceae and Iponwea pes-caprae (Convolvulaceae and the robust stoloniferous grass Spinijex littoreus, the last having an important function for the fonnation of coastal dunes.

SPINIFICI-SCAEVOLETEA SERICEAE, A NEW VEGETATION CLASS FOR PSAMMOPHYTIC DUNE VEGETATION IN THAILAND

Directory of Open Access Journals (Sweden)

S. PIGNATII

1996-01-01

Full Text Available This is a short account on the coastal dune vegetation of the Gulf of Siam in Thailand. Vegetation is mainly composed by succulent creeping plants with herbaceous habit as to Canavalia maritima (Papilionaceae and Iponwea pes-caprae (Convolvulaceae and the robust stoloniferous grass Spinijex littoreus, the last having an important function for the fonnation of coastal dunes.

Oscillations in a simple climate–vegetation model

Directory of Open Access Journals (Sweden)

J. Rombouts

2015-05-01

Full Text Available We formulate and analyze a simple dynamical systems model for climate–vegetation interaction. The planet we consider consists of a large ocean and a land surface on which vegetation can grow. The temperature affects vegetation growth on land and the amount of sea ice on the ocean. Conversely, vegetation and sea ice change the albedo of the planet, which in turn changes its energy balance and hence the temperature evolution. Our highly idealized, conceptual model is governed by two nonlinear, coupled ordinary differential equations, one for global temperature, the other for vegetation cover. The model exhibits either bistability between a vegetated and a desert state or oscillatory behavior. The oscillations arise through a Hopf bifurcation off the vegetated state, when the death rate of vegetation is low enough. These oscillations are anharmonic and exhibit a sawtooth shape that is characteristic of relaxation oscillations, as well as suggestive of the sharp deglaciations of the Quaternary. Our model's behavior can be compared, on the one hand, with the bistability of even simpler, Daisyworld-style climate–vegetation models. On the other hand, it can be integrated into the hierarchy of models trying to simulate and explain oscillatory behavior in the climate system. Rigorous mathematical results are obtained that link the nature of the feedbacks with the nature and the stability of the solutions. The relevance of model results to climate variability on various timescales is discussed.

Oscillations in a simple climate-vegetation model

Science.gov (United States)

Rombouts, J.; Ghil, M.

2015-05-01

We formulate and analyze a simple dynamical systems model for climate-vegetation interaction. The planet we consider consists of a large ocean and a land surface on which vegetation can grow. The temperature affects vegetation growth on land and the amount of sea ice on the ocean. Conversely, vegetation and sea ice change the albedo of the planet, which in turn changes its energy balance and hence the temperature evolution. Our highly idealized, conceptual model is governed by two nonlinear, coupled ordinary differential equations, one for global temperature, the other for vegetation cover. The model exhibits either bistability between a vegetated and a desert state or oscillatory behavior. The oscillations arise through a Hopf bifurcation off the vegetated state, when the death rate of vegetation is low enough. These oscillations are anharmonic and exhibit a sawtooth shape that is characteristic of relaxation oscillations, as well as suggestive of the sharp deglaciations of the Quaternary. Our model's behavior can be compared, on the one hand, with the bistability of even simpler, Daisyworld-style climate-vegetation models. On the other hand, it can be integrated into the hierarchy of models trying to simulate and explain oscillatory behavior in the climate system. Rigorous mathematical results are obtained that link the nature of the feedbacks with the nature and the stability of the solutions. The relevance of model results to climate variability on various timescales is discussed.

Estimating fractional vegetation cover and the vegetation index of bare soil and highly dense vegetation with a physically based method

Science.gov (United States)

Song, Wanjuan; Mu, Xihan; Ruan, Gaiyan; Gao, Zhan; Li, Linyuan; Yan, Guangjian

2017-06-01

Normalized difference vegetation index (NDVI) of highly dense vegetation (NDVIv) and bare soil (NDVIs), identified as the key parameters for Fractional Vegetation Cover (FVC) estimation, are usually obtained with empirical statistical methods However, it is often difficult to obtain reasonable values of NDVIv and NDVIs at a coarse resolution (e.g., 1 km), or in arid, semiarid, and evergreen areas. The uncertainty of estimated NDVIs and NDVIv can cause substantial errors in FVC estimations when a simple linear mixture model is used. To address this problem, this paper proposes a physically based method. The leaf area index (LAI) and directional NDVI are introduced in a gap fraction model and a linear mixture model for FVC estimation to calculate NDVIv and NDVIs. The model incorporates the Moderate Resolution Imaging Spectroradiometer (MODIS) Bidirectional Reflectance Distribution Function (BRDF) model parameters product (MCD43B1) and LAI product, which are convenient to acquire. Two types of evaluation experiments are designed 1) with data simulated by a canopy radiative transfer model and 2) with satellite observations. The root-mean-square deviation (RMSD) for simulated data is less than 0.117, depending on the type of noise added on the data. In the real data experiment, the RMSD for cropland is 0.127, for grassland is 0.075, and for forest is 0.107. The experimental areas respectively lack fully vegetated and non-vegetated pixels at 1 km resolution. Consequently, a relatively large uncertainty is found while using the statistical methods and the RMSD ranges from 0.110 to 0.363 based on the real data. The proposed method is convenient to produce NDVIv and NDVIs maps for FVC estimation on regional and global scales.

Plant genotype-specific archaeal and bacterial endophytes but similar Bacillus antagonists colonize Mediterranean olive trees

Directory of Open Access Journals (Sweden)

Henry eMueller

2015-03-01

Full Text Available Endophytes have an intimate and often symbiotic interaction with their hosts. Less is known about the composition and function of endophytes in trees. In order to evaluate our hypothesis that plant genotype and origin have a strong impact on both, endophytes of leaves from 10 Olea europaea L. cultivars from the Mediterranean basin growing at a single agricultural site in Spain and from nine wild olive trees located in natural habitats in Greece, Cyprus and on Madeira Island were studied. The composition of the bacterial endophytic communities as revealed by 16S rRNA gene amplicon sequencing and the subsequent PCoA analysis showed a strong correlation to the plant genotypes. The bacterial distribution patterns were congruent with the plant origins in Eastern and Western areas of the Mediterranean basin. Subsequently, the endophytic microbiome of wild olives was shown to be closely related to those of cultivated olives of the corresponding geographic origins. The olive leaf endosphere harbored mostly Proteobacteria, followed by Firmicutes, Actinobacteria and Bacteroidetes. The detection of a high portion of archaeal taxa belonging to the phyla Thaumarchaeota, Crenarchaeota and Euryarchaeota in the amplicon libraries was an unexpected discovery, which was confirmed by quantitative real-time PCR revealing an archaeal portion of up to 35.8%. Although the function of these Archaea for their host plant remains speculative, this finding suggests a significant relevance of archaeal endophytes for plant-microbe interactions. In addition, the antagonistic potential of culturable endophytes was determined; all isolates with antagonistic activity against the olive-pathogenic fungus Verticillium dahliae Kleb. belong to Bacillus amyloliquefaciens. In contrast to the specific global structural diversity, BOX-fingerprints of the antagonistic Bacillus isolates were highly similar and independent of the olive genotype from which they were isolated.

Impacts of Vegetation Growth on Reach-scale Flood Hydraulics in a Sand-bed River and the Implications for Vegetation-morphology Coevolution

Science.gov (United States)

Box, S.; Wilcox, A. C.

2017-12-01

Vegetation alters flood hydraulics and geomorphic response, yet quantifying and predicting such responses across spatial and temporal scales remains challenging. Plant- and patch-scale studies consistently show that vegetation increases local hydraulic variability, yet reach-scale hydrodynamic models often assume vegetation has a spatially homogeneous effect on hydraulics. Using Nays2DH in iRIC (International River Interface Cooperative), we model the effect of spatially heterogeneous vegetation on a series of floods with varying antecedent vegetation conditions in a sand-bed river in western Arizona, taking advantage of over a decade of data on a system that experienced substantial geomorphic, hydrologic, and ecosystem changes. We show that pioneer woody seedlings (Tamarix, Populus, Salix) and cattail (Typha) increase local hydraulic variability, including velocity and bed shear stress, along individual cross sections, predominantly by decreasing velocity in zones of vegetation establishment and growth and increasing velocity in unvegetated areas, with analogous effects on shear stress. This was especially prominent in a study reach where vegetation growth contributed to thalweg incision relative to a vegetated bar. Evaluation of these results in the context of observed geomorphic response to floods elucidates mechanisms by which vegetation and channel morphology coevolve at a reach scale. By quantifying the influence of spatially heterogeneous vegetation on reach-scale hydraulics, we demonstrate that plant- and patch-scale research on vegetation hydraulics is applicable to ecogeomorphology at the reach scale.

Assessment of vegetable production practices in Qwaqwa within ...

African Journals Online (AJOL)

In this study, the information such as biographical information of the vegetable farmers, information about the vegetable gardens or farms, the current vegetable farming practices, irrigation practices on the vegetable soil and the farmers' physical and financial records were assessed. The assessment was conducted through ...

Radiation and the vegetable industry

International Nuclear Information System (INIS)

Roberts, P.B.

1984-01-01

The possible uses of irradiation in the vegetable industry are considered. Interest has been increasing because of possible bans on chemical fumigants and clearance of irradiation as an acceptable process, up to certain dose limits, by Codex Alimentarius and the US FDA. Inhibition of sprouting in potatoes and onions would be one possibility for exploitation. However, the main incentive for vegetable irradiation would be as a quarantine treatment for exported products. The shelf-life of a few vegetables could also be increased by combining radiation with heat treatments. Costs in New Zealand and consumer attitudes are briefly considered

Post Fire Vegetation Recovery in Portugal

Science.gov (United States)

Gouveia, Celia; Bastos, Ana; DaCamara, Carlos; Trigo, Ricardo M.

2011-01-01

Fires in Portugal, as in the Mediterranean ecosystems, have a complex effect on vegetation regeneration due to the different responses of vegetation to the variety of fire regimes and to the complexity of landscape structures. A thorough evaluation of vegetation recovery after fire events becomes therefore crucial in land management. In 2005, Portugal suffered a strong damage from forest fires that damaged an area of 300 000 ha of forest and shrub. This year are particularly interesting because it is associated the severe drought of 2005. The aim of the present study is to identify large burnt scars in Portugal during the 2005 fire seasons and monitoring vegetation behaviour throughout the pre and the post fire periods. The mono-parametric model developed by Gouveia et al. (2010), based on monthly values of NDVI, at the 1km×1km spatial scale, as obtained from the VEGETATION-SPOT5 instrument, from 1999 to 2009, was used.

Vegetable oil spills : oil properties and behaviour

International Nuclear Information System (INIS)

Fingas, M.; Fieldhouse, B.; Jokuty, P.

2001-01-01

In 1997, the United States Environmental Protection Agency conducted a thorough review of the issue regarding vegetable oil spills. Recent attention has refocused on this issue as a result of an incident where 20 tons of canola oil was spilled in the Vancouver Harbour in 2000. In the past, vegetable oils were suggested to be a useful test material because they were thought to be innocuous. It was even suggested they be used to remove petroleum oil residues from beaches. However, recent studies have shown that spills of vegetable oils can have major environmental consequences, equivalent to those of petroleum oil spills. The spills have devastating effects on birds and intertidal organisms. This paper presented a summary of historical vegetable spills from around the world. In this study, specific behaviour tests were examined for several oils including canola, soy bean, olive, castor and corn oils. Evaporation, water-in-oil emulsification and chemical dispersion were measured and were found to be nearly zero, suggesting that vegetable oil spills are not very soluble in water. The aquatic toxicity of vegetable oil is low, but their fate is quite different from petroleum. Vegetable oils do not evaporate to a significant degree, they do not form water-in-oil emulsions, nor do they disperse in water. The physical properties of vegetable oils were also measured, including density and viscosity. This paper presented the aquatic toxicity of several vegetable oils along with other environmental data including the degradation rates noted in the literature. Most environmental damage reported in the literature is by contact with birds feathers resulting in hypothermia and secondly by smothering of intertidal organisms. The effect of vegetable oil on fish has not been well studied, but it is expected that there will be little destructive effect except where smothering can occur. 35 refs., 3 tabs

Vegetable oil spills : oil properties and behaviour

Energy Technology Data Exchange (ETDEWEB)

Fingas, M.; Fieldhouse, B.; Jokuty, P. [Environment Canada, Ottawa, ON (Canada). Emergencies Science Div

2001-07-01

In 1997, the United States Environmental Protection Agency conducted a thorough review of the issue regarding vegetable oil spills. Recent attention has refocused on this issue as a result of an incident where 20 tons of canola oil was spilled in the Vancouver Harbour in 2000. In the past, vegetable oils were suggested to be a useful test material because they were thought to be innocuous. It was even suggested they be used to remove petroleum oil residues from beaches. However, recent studies have shown that spills of vegetable oils can have major environmental consequences, equivalent to those of petroleum oil spills. The spills have devastating effects on birds and intertidal organisms. This paper presented a summary of historical vegetable spills from around the world. In this study, specific behaviour tests were examined for several oils including canola, soy bean, olive, castor and corn oils. Evaporation, water-in-oil emulsification and chemical dispersion were measured and were found to be nearly zero, suggesting that vegetable oil spills are not very soluble in water. The aquatic toxicity of vegetable oil is low, but their fate is quite different from petroleum. Vegetable oils do not evaporate to a significant degree, they do not form water-in-oil emulsions, nor do they disperse in water. The physical properties of vegetable oils were also measured, including density and viscosity. This paper presented the aquatic toxicity of several vegetable oils along with other environmental data including the degradation rates noted in the literature. Most environmental damage reported in the literature is by contact with birds feathers resulting in hypothermia and secondly by smothering of intertidal organisms. The effect of vegetable oil on fish has not been well studied, but it is expected that there will be little destructive effect except where smothering can occur. 35 refs., 3 tabs.

Formalized classification of species-poor vegetation: a proposal of a consistent protocol for aquatic vegetation

Czech Academy of Sciences Publication Activity Database

Landucci, F.; Tichý, L.; Šumberová, Kateřina; Chytrý, M.

2015-01-01

Roč. 26, č. 4 (2015), s. 791-803 ISSN 1100-9233 R&D Projects: GA ČR GB14-36079G Institutional support: RVO:67985939 Keywords : vegetation classification * vegetation database * Coctail method Subject RIV: EF - Botanics Impact factor: 3.151, year: 2015

Wieslander Vegetation

Data.gov (United States)

California Natural Resource Agency — Digital version of the 1945 California Vegetation Type Maps by A. E. Wieslander of the U.S. Forest Service. Source scale of maps are 1:100,000. These compiled maps...

Vegetation - McKenzie Preserve [ds703

Data.gov (United States)

California Natural Resource Agency — The California Native Plant Society (CNPS) Vegetation Program produced a vegetation map and classification for approximately 11,600 acres primarily within Millerton...

Increasing portion sizes of fruits and vegetables in an elementary school lunch program can increase fruit and vegetable consumption.

Science.gov (United States)

Miller, Nicole; Reicks, Marla; Redden, Joseph P; Mann, Traci; Mykerezi, Elton; Vickers, Zata

2015-08-01

Increasing portion size can increase children's consumption of food. The goal of this study was to determine whether increasing the portion sizes of fruits and vegetables in an elementary school cafeteria environment would increase children's consumption of them. We measured each child's consumption of the fruit and vegetables served in a cafeteria line on a control day (normal cafeteria procedures) and on two intervention days. When we increased the portion size of 3 of the 4 fruits and vegetables by about 50%, children who took those foods increased their consumption of them. Although this was an effective strategy for increasing fruit and vegetable consumption among students who took those foods, many children chose not to take any fruits or vegetables. Further efforts are needed to increase children's selection and consumption of fruits and vegetables in an environment of competing foods of higher palatability. Copyright © 2015 Elsevier Ltd. All rights reserved.

East African Cenozoic vegetation history.

Science.gov (United States)

Linder, Hans Peter

2017-11-01

The modern vegetation of East Africa is a complex mosaic of rainforest patches; small islands of tropic-alpine vegetation; extensive savannas, ranging from almost pure grassland to wooded savannas; thickets; and montane grassland and forest. Here I trace the evolution of these vegetation types through the Cenozoic. Paleogene East Africa was most likely geomorphologically subdued and, as the few Eocene fossil sites suggest, a woodland in a seasonal climate. Woodland rather than rainforest may well have been the regional vegetation. Mountain building started with the Oligocene trap lava flows in Ethiopia, on which rainforest developed, with little evidence of grass and none of montane forests. The uplift of the East African Plateau took place during the middle Miocene. Fossil sites indicate the presence of rainforest, montane forest and thicket, and wooded grassland, often in close juxtaposition, from 17 to 10 Ma. By 10 Ma, marine deposits indicate extensive grassland in the region and isotope analysis indicates that this was a C 3 grassland. In the later Miocene rifting, first of the western Albertine Rift and then of the eastern Gregory Rift, added to the complexity of the environment. The building of the high strato-volcanos during the later Mio-Pliocene added environments suitable for tropic-alpine vegetation. During this time, the C 3 grassland was replaced by C 4 savannas, although overall the extent of grassland was reduced from the mid-Miocene high to the current low level. Lake-level fluctuations during the Quaternary indicate substantial variation in rainfall, presumably as a result of movements in the intertropical convergence zone and the Congo air boundary, but the impact of these fluctuations on the vegetation is still speculative. I argue that, overall, there was an increase in the complexity of East African vegetation complexity during the Neogene, largely as a result of orogeny. The impact of Quaternary climatic fluctuation is still poorly understood

Internalisation of microbes in vegetables: microbial load of Ghanaian vegetables and the relationship with different water sources of irrigation.

Science.gov (United States)

Donkor, Eric S; Lanyo, R; Kayang, Boniface B; Quaye, Jonathan; Edoh, Dominic A

2010-09-01

The occurrence of pathogens in the internal parts of vegetables is usually associated with irrigation water or contaminated soil and could pose risk to consumers as the internalised pathogens are unaffected by external washing. This study was carried out to assess the rate of internalisation of microbes in common Ghanaian vegetables. Standard microbiological methods were employed in microbial enumeration of vegetables collected at the market and farm levels, as well as irrigation water and soil samples. The overall mean counts of vegetables were 4.0 x 10(3) cfu g(-1); 8.1 x 10(2) cfu g(-1); 2.0 x 10(2) cfu g(-1); 3.5 x 10(2) cfu g(-1) for total bacteria, coliform counts, faecal coliform counts and yeast counts, respectively. The rate of internalisation of coliforms in vegetables irrigated with stream/well water was 2.7 times higher than those irrigated with pipe water. The mean coliform counts (4.7 x 10(7) cfu g(-1)) and faecal coliform counts (1.8 x 10(6) cfu g(-1)) of soil samples were similar to those of stream water suggesting both sources exerted similar contamination rates on the vegetables. Generally, there were no significant variations between the rates of internalisation of microbes at the market and farm levels at p vegetables mainly occurred at the farm level. The study has shown that microbial contamination of vegetables in Ghana is not limited to the external surface, but internal vegetable parts could harbour high microbial loads and pose risk to consumers. Safety practices associated with the commodity should therefore not be limited to external washing only. There is the additional need of heating vegetables to eliminate microbes both externally and internally before consumption.

Discharge/Stage Relations in Vegetated Danish Streams

DEFF Research Database (Denmark)

Larsen, Torben; Frier, Jens-Ole; Vestergaard, Kristian

1990-01-01

This paper describes how the friction in danish streams varies as function of the vegetation. The major species of vegetation are represented. A series of laboratory and field experiments are described, and a hypothesis for the influence of the vegetation on the Manning's n is discussed....

Mapping and characterizing the vegetation types of the Democratic Republic of Congo using SPOT VEGETATION time series

Science.gov (United States)

Vancutsem, C.; Pekel, J.-F.; Evrard, C.; Malaisse, F.; Defourny, P.

2009-02-01

The need for quantitative and accurate information to characterize the state and evolution of vegetation types at a national scale is widely recognized. This type of information is crucial for the Democratic Republic of Congo, which contains the majority of the tropical forest cover of Central Africa and a large diversity of habitats. In spite of recent progress in earth observation capabilities, vegetation mapping and seasonality analysis in equatorial areas still represent an outstanding challenge owing to high cloud coverage and the extent and limited accessibility of the territory. On one hand, the use of coarse-resolution optical data is constrained by performance in the presence of cloud screening and by noise arising from the compositing process, which limits the spatial consistency of the composite and the temporal resolution. On the other hand, the use of high-resolution data suffers from heterogeneity of acquisition dates, images and interpretation from one scene to another. The objective of the present study was to propose and demonstrate a semi-automatic processing method for vegetation mapping and seasonality characterization based on temporal and spectral information from SPOT VEGETATION time series. A land cover map with 18 vegetation classes was produced using the proposed method that was fed by ecological knowledge gathered from botanists and reference documents. The floristic composition and physiognomy of each vegetation type are described using the Land Cover Classification System developed by the FAO. Moreover, the seasonality of each class is characterized on a monthly basis and the variation in different vegetation indicators is discussed from a phenological point of view. This mapping exercise delivers the first area estimates of seven different forest types, five different savannas characterized by specific seasonality behavior and two aquatic vegetation types. Finally, the result is compared to two recent land cover maps derived from

The Hydromechanics of Vegetation for Slope Stabilization

Science.gov (United States)

Mulyono, A.; Subardja, A.; Ekasari, I.; Lailati, M.; Sudirja, R.; Ningrum, W.

2018-02-01

Vegetation is one of the alternative technologies in the prevention of shallow landslide prevention that occurs mostly during the rainy season. The application of plant for slope stabilization is known as bioengineering. Knowledge of the vegetative contribution that can be considered in bioengineering was the hydrological and mechanical aspects (hydromechanical). Hydrological effect of the plant on slope stability is to reduce soil water content through transpiration, interception, and evapotranspiration. The mechanical impact of vegetation on slope stability is to stabilize the slope with mechanical reinforcement of soils through roots. Vegetation water consumption varies depending on the age and density, rainfall factors and soil types. Vegetation with high ability to absorb water from the soil and release into the atmosphere through a transpiration process will reduce the pore water stress and increase slope stability, and vegetation with deep root anchoring and strong root binding was potentially more significant to maintain the stability of the slope.

Parasitic Contamination of Raw Vegetables in Shahroud, Semnan

Directory of Open Access Journals (Sweden)

Saeid Nazemi

2012-08-01

Full Text Available Background: Given the importance of healthy vegetables, the present study was conducted to determine parasitic infection of vegetable consumed in Shahroud.Materials and Methods: This cross-sectional study has been conducted on 92 samples of various vegetables collected from 16 vegetable growing farms and 1 vegetable process workshop. Results: Sixty two percent of tested vegetables lacked parasites and the highest amount of parasites observed (34.78% was related to Giardia lamblia. A significant relationship was observed between parasite and having toilets in the farms as well as the extent of farms.Conclusion: safety of fertilizers consumed by farms and healthy fruits can have an effective role in reducing the parasitic infections.

Effects of Telecoupling on Global Vegetation Dynamics

Science.gov (United States)

Viña, A.; Liu, J.

2016-12-01

With the ever increasing trend in telecoupling processes, such as international trade, all countries around the world are becoming more interdependent. However, the effects of this growing interdependence on vegetation (e.g., shifts in the geographic extent and distribution) remain unknown even though vegetation dynamics are crucially important for food production, carbon sequestration, provision of other ecosystem services, and biodiversity conservation. In this study we evaluate the effects of international trade on the spatio-temporal trajectories of vegetation at national and global scales, using vegetation index imagery collected over more than three decades by the Advanced Very High Resolution Radiometer (AVHRR) satellite sensor series together with concurrent national and international data on international trade (and its associated movement of people, goods, services and information). The spatio-temporal trajectories of vegetation are obtained using the scale of fluctuation technique, which is based on the decomposition of the AVHRR image time series to obtain information on its spatial dependence structure over time. Similar to the correlation length, the scale of fluctuation corresponds to the range over which fluctuations in the vegetation index are spatially correlated. Results indicate that global vegetation has changed drastically over the last three decades. These changes are not uniform across space, with hotspots in active trading countries. This study not only has direct implications for understanding global vegetation dynamics, but also sheds important insights on the complexity of human-nature interactions across telecoupled systems.

9 CFR 319.311 - Chow mein vegetables with meat, and chop suey vegetables with meat.

Science.gov (United States)

2010-01-01

... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Chow mein vegetables with meat, and chop suey vegetables with meat. 319.311 Section 319.311 Animals and Animal Products FOOD SAFETY AND INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE AGENCY ORGANIZATION AND TERMINOLOGY; MANDATORY MEAT AND POULTRY...

Ecosystems past: prehistory of California vegetation

Science.gov (United States)

C.I. Millar; W.B. Woolfenden

2016-01-01

The history of California's vegetation, from origins in the Mesozoic through Quaternary is outlined. Climatic and geologic history and the processes driving changes in vegetation over time are also described. 

Simulating vegetation dynamics in Chile from 21ka BP to present: Effects of climate change on vegetation functions and cover

Science.gov (United States)

Werner, Christian; Liakka, Johan; Schmid, Manuel; Fuentes, Juan-Pablo; Ehlers, Todd A.; Hickler, Thomas

2017-04-01

Vegetation composition and establishment is strongly dependent on climate conditions but also a result of vegetation dynamics (competition for light, water and nutrients). In addition, vegetation exerts control over the development of landscapes as it mediates the climatic and hydrological forces shaping the terrain via hillslope and fluvial processes. At the same time, topography as well as soil texture and soil depth affect the microclimate, soil water storage and rooting space that is defining the environmental envelope for vegetation development. Within the EarthShape research program (www.earthshape.net) we evaluate these interactions by simulating the co-evolution of landscape and vegetation with a dynamic vegetation model (LPJ-GUESS) and a landscape evolution model (LandLab). LPJ-GUESS is a mechanistic model driven by daily or monthly weather data and explicitly simulates vegetation physiology, succession, competition and water and nutrient cycling. Here we present the results of first transient vegetation simulations from 21kyr BP to present-day using the TraCE-21ka climate dataset for four focus sites along the coastal cordillera of Chile that are exposed to a substantial meridional climate gradient (ranging from hyper-arid to humid-temperate conditions). We show that the warming occurring in the region from LGM to present, in addition to the increase of atmospheric CO2 concentrations, led to a shift in vegetation composition and surface cover. Future work will show how these changes resonate in the dynamics of hillslope and fluvial erosion and ultimately bi-directional feedback mechanisms of vegetation development and landscape evolution/ soil formation (see also companion presentation by Schmid et al., this session).

Seasonally asymmetric enhancement of northern vegetation productivity

Science.gov (United States)

Park, T.; Myneni, R.

2017-12-01

Multiple evidences of widespread greening and increasing terrestrial carbon uptake have been documented. In particular, enhanced gross productivity of northern vegetation has been a critical role leading to observed carbon uptake trend. However, seasonal photosynthetic activity and its contribution to observed annual carbon uptake trend and interannual variability are not well understood. Here, we introduce a multiple-source of datasets including ground, atmospheric and satellite observations, and multiple process-based global vegetation models to understand how seasonal variation of land surface vegetation controls a large-scale carbon exchange. Our analysis clearly shows a seasonally asymmetric enhancement of northern vegetation productivity in growing season during last decades. Particularly, increasing gross productivity in late spring and early summer is obvious and dominant driver explaining observed trend and variability. We observe more asymmetric productivity enhancement in warmer region and this spatially varying asymmetricity in northern vegetation are likely explained by canopy development rate, thermal and light availability. These results imply that continued warming may facilitate amplifying asymmetric vegetation activity and cause these trends to become more pervasive, in turn warming induced regime shift in northern land.

Vegetation - San Felipe Valley [ds172

Data.gov (United States)

California Natural Resource Agency — This Vegetation Map of the San Felipe Valley Wildlife Area in San Diego County, California is based on vegetation samples collected in the field in 2002 and 2005 and...

Vegetation study in support of the design and optimization of vegetative soil covers, Sandia National Laboratories, Albuquerque, New Mexico.

Energy Technology Data Exchange (ETDEWEB)

Peace, Gerald (Jerry) L.; Goering, Timothy James (GRAM inc., Albuquerque, NM); Knight, Paul J. (Marron and Associates, Albuquerque, NM); Ashton, Thomas S. (Marron and Associates, Albuquerque, NM)

2004-11-01

A vegetation study was conducted in Technical Area 3 at Sandia National Laboratories, Albuquerque, New Mexico in 2003 to assist in the design and optimization of vegetative soil covers for hazardous, radioactive, and mixed waste landfills at Sandia National Laboratories/New Mexico and Kirtland Air Force Base. The objective of the study was to obtain site-specific, vegetative input parameters for the one-dimensional code UNSAT-H and to identify suitable, diverse native plant species for use on vegetative soil covers that will persist indefinitely as a climax ecological community with little or no maintenance. The identification and selection of appropriate native plant species is critical to the proper design and long-term performance of vegetative soil covers. Major emphasis was placed on the acquisition of representative, site-specific vegetation data. Vegetative input parameters measured in the field during this study include root depth, root length density, and percent bare area. Site-specific leaf area index was not obtained in the area because there was no suitable platform to measure leaf area during the 2003 growing season due to severe drought that has persisted in New Mexico since 1999. Regional LAI data was obtained from two unique desert biomes in New Mexico, Sevilletta Wildlife Refuge and Jornada Research Station.

On the characterization of vegetation recovery after fire disturbance using Fisher-Shannon analysis and SPOT/VEGETATION Normalized Difference Vegetation Index (NDVI) time series

Science.gov (United States)

Lasaponara, Rosa; Lanorte, Antonio; Lovallo, Michele; Telesca, Luciano

2015-04-01

Time series can fruitfully support fire monitoring and management from statistical analysis of fire occurrence (Tuia et al. 2008) to danger estimation (lasaponara 2005), damage evaluation (Lanorte et al 2014) and post fire recovery (Lanorte et al. 2014). In this paper, the time dynamics of SPOT-VEGETATION Normalized Difference Vegetation Index (NDVI) time series are analyzed by using the statistical approach of the Fisher-Shannon (FS) information plane to assess and monitor vegetation recovery after fire disturbance. Fisher-Shannon information plane analysis allows us to gain insight into the complex structure of a time series to quantify its degree of organization and order. The analysis was carried out using 10-day Maximum Value Composites of NDVI (MVC-NDVI) with a 1 km × 1 km spatial resolution. The investigation was performed on two test sites located in Galizia (North Spain) and Peloponnese (South Greece), selected for the vast fires which occurred during the summer of 2006 and 2007 and for their different vegetation covers made up mainly of low shrubland in Galizia test site and evergreen forest in Peloponnese. Time series of MVC-NDVI have been analyzed before and after the occurrence of the fire events. Results obtained for both the investigated areas clearly pointed out that the dynamics of the pixel time series before the occurrence of the fire is characterized by a larger degree of disorder and uncertainty; while the pixel time series after the occurrence of the fire are featured by a higher degree of organization and order. In particular, regarding the Peloponneso fire, such discrimination is more evident than in the Galizia fire. This suggests a clear possibility to discriminate the different post-fire behaviors and dynamics exhibited by the different vegetation covers. Reference Lanorte A, R Lasaponara, M Lovallo, L Telesca 2014 Fisher-Shannon information plane analysis of SPOT/VEGETATION Normalized Difference Vegetation Index (NDVI) time series to

Exploring vegetation in the fourth dimension.

Science.gov (United States)

Mitchell, Fraser J G

2011-01-01

Much ecological research focuses on changes in vegetation on spatial scales from stands to landscapes; however, capturing data on vegetation change over relevant timescales remains a challenge. Pollen analysis offers unrivalled access to data with global coverage over long timescales. Robust techniques have now been developed that enable pollen data to be converted into vegetation data in terms of individual taxa, plant communities or biomes, with the possibility of deriving from those data a range of plant attributes and ecological indicators. In this review, I discuss how coupling pollen with macrofossil, charcoal and genetic data opens up the extensive pollen databases to investigation of the drivers of vegetation change over time and also provides extensive data sets for testing hypotheses with wide ecological relevance. © 2010 Elsevier Ltd. All rights reserved.

Theory, methods and tools for determining environmental flows for riparian vegetation: Riparian vegetation-flow response guilds

Science.gov (United States)

Merritt, D.M.; Scott, M.L.; Leroy, Poff N.; Auble, G.T.; Lytle, D.A.

2010-01-01

Riparian vegetation composition, structure and abundance are governed to a large degree by river flow regime and flow-mediated fluvial processes. Streamflow regime exerts selective pressures on riparian vegetation, resulting in adaptations (trait syndromes) to specific flow attributes. Widespread modification of flow regimes by humans has resulted in extensive alteration of riparian vegetation communities. Some of the negative effects of altered flow regimes on vegetation may be reversed by restoring components of the natural flow regime. 2. Models have been developed that quantitatively relate components of the flow regime to attributes of riparian vegetation at the individual, population and community levels. Predictive models range from simple statistical relationships, to more complex stochastic matrix population models and dynamic simulation models. Of the dozens of predictive models reviewed here, most treat one or a few species, have many simplifying assumptions such as stable channel form, and do not specify the time-scale of response. In many cases, these models are very effective in developing alternative streamflow management plans for specific river reaches or segments but are not directly transferable to other rivers or other regions. 3. A primary goal in riparian ecology is to develop general frameworks for prediction of vegetation response to changing environmental conditions. The development of riparian vegetation-flow response guilds offers a framework for transferring information from rivers where flow standards have been developed to maintain desirable vegetation attributes, to rivers with little or no existing information. 4. We propose to organise riparian plants into non-phylogenetic groupings of species with shared traits that are related to components of hydrologic regime: life history, reproductive strategy, morphology, adaptations to fluvial disturbance and adaptations to water availability. Plants from any river or region may be grouped

Heavy Metals Accumulation Characteristics of Vegetables in Hangzhou City, China

Directory of Open Access Journals (Sweden)

GU Yan-qing

2015-08-01

Full Text Available A field survey of heavy metal concentrations in soils and vegetables grown in 30 vegetable farmlands of Hangzhou City were carried out. Through calculating the bioconcentration factor(BCFand transfer factor(TFfor different heavy metals(Cu, Zn, Cd, Cr and Pbin 27 kinds of different vegetables which belong to leafy vegetables, root vegetables or eggplant fruit vegetables, assessing their accumulation characteristics of heavy metals according to the differences of the bio-concentration factor, the reasonable proposals were put forward to optimize the planting structure of vegetables in mild and middle-level heavy metal contamination soils. The experimental results were as follows: In soils with mild and middle-level heavy metal contamination, leafy vegetables, such as crown daisy, cabbage, celery and Chinese long cabbage, had relatively low enrichment ability of heavy metals, so as the root and fruit vegetables like white radish, carrot, tomatoes, hence these vegetables could be planted preferentially. In contrast, some kinds of vegetables, including white amaranth, red amaranth, tatsoi, broccoli, gynura, brassica juncea and lettuce of leafy vegetables, lactuca sativa, taro, red radish and cherry radish of rhizome vegetables and sweet pepper of fruit vegetables, had relatively high accumulation ability of heavy metal, which should be avoided to be planted in soils with mild and middle-level heavy metal contamination.

Nigerian women reap benefits from indigenous vegetables | IDRC ...

International Development Research Centre (IDRC) Digital Library (Canada)

2016-06-06

Jun 6, 2016 ... Demand for fresh indigenous vegetables in Nigeria has increased ... greater returns from indigenous vegetables compared to conventional vegetables. ... In Kolli Hills, Tamil Nadu, monocropping of a single, non-edible variety ...

Determination of zinc contents in vegetables

International Nuclear Information System (INIS)

Salah-ud-Din; Salariya, A.M.; Yasin, M.

1996-01-01

Zinc content of three groups of vegetables (roots and tuber, leaves and fruits) collected from local markets was determined and are reported here. The determination was made by Atomic Absorption Spectrophotometer. The results obtained show that the zinc content of different vegetables ranged from 6.26-36.80 ppm, 8.80-70-70 ppm and 7.20-35.10 ppm for roots and tubers, fruits of vegetables respectively on dry weight basis. Generally, the values obtained in majority are not above, the maximum permissible limits. (author)

Radionuclide interception and loss processes in vegetation

International Nuclear Information System (INIS)

Proehl, G.; Hoffman, F.O.

1996-01-01

Data available since the Chernobyl accident have strengthened the view that the transfer of radionuclides from air to vegetation is a primary area of uncertainty in the estimation of the contamination of food chains leading to human exposure. The processes affecting the overall transfer from air to vegetation involve wet and dry deposition, interception and initial retention, and post-deposition retention of radioactive substances by vegetation. During the growing season, the time-integrated concentrations of radionuclides on vegetation in the first few months after initial deposition are dominated by the direct foliar interception of deposited material. Chapter 2 contains a review of data for modelling the direct foliar interception and initial retention of radioactivity deposited by dry and wet processes, together with data on the factors affecting post-deposition retention of radioactivity on the vegetation. 82 refs, 9 figs, 11 tabs

Mineral composition of non-conventional leafy vegetables.

Science.gov (United States)

Barminas, J T; Charles, M; Emmanuel, D

1998-01-01

Six non-conventional leafy vegetables consumed largely by the rural populace of Nigeria were analyzed for mineral composition. Mineral contents appeared to be dependent on the type of vegetables. Amaranthus spinosus and Adansonia digitata leaves contained the highest level of iron (38.4 mg/100 g and 30.6 mg/100 g dw, respectively). These values are low compared to those for common Nigerian vegetables but higher than those for other food sources. All the vegetables contained high levels of calcium compared to common vegetables, thus they could be a rich source of this mineral. Microelement content of the leaves varied appreciably. Zinc content was highest in Moringa oleifera, Adansonia digitata and Cassia tora leaves (25.5 mg/100 g, 22.4 mg/100 g and 20.9 mg/100 g dw, respectively) while the manganese content was comparatively higher in Colocasia esculenta. The concentrations of the mineral elements in the vegetables per serving portion are presented and these values indicate that the local vegetables could be valuable and important contributors in the diets of the rural and urban people of Nigeria. The mean daily intake of P, Mg, Ca, Fe, Cu and Zn were lower than their recommended dietary allowances (RDAs). However, the manganese daily intake was found not to differ significantly (p = 0.05) from the RDA value.

Transfer of 137Cs to wild vegetables

International Nuclear Information System (INIS)

Ito, Nobuhiko; Natsuhori, Masahiro; Mezawa, Akane; Kawakami, Akira

1998-01-01

For the evaluation of internal radiation dose, it is needed to estimate the amount of radionuclide incorporated to human body using a simulation model. 137 Cesium (Cs) is easily transferred associating with food intake as well as potassium and so, Cs is an important nuclide for evaluation of internal radiation. 137 Cs concentrations in wild vegetables are higher than those of cultured vegetables and milk. Therefore, the transfer coefficients of 137 Cs from soil to wild vegetables were estimated in this study. Wild vegetables and soils of their farms were collected in the Hakkoda Mountain range of Aomori Prefecture. The levels of 137 Cs in wild vegetables were 0.42-18.35 (Bq/kg), whereas those in cabbage and spinach were 0.08 and 0.01 (Bq/kg), respectively, indicating that the Cs level is dozens to several hundreds times higher in wild vegetables than cultured ones. And the transfer coefficient was estimated as 0.003-0.94 for the former and 0.001-0.8 for the latter. On the other hand, 1 37 Cs levels of the soils on which wild vegetables grew was 28.0 Bq/kg and it was 3.9 Bq/kg for the farm soil. Furthermore, the effects of water content and pH of the soil on the transfer coefficient were studied. (M.N.)

Vegetation geographical patterns as a key to the past, with emphasis on the dry vegetation types of South Africa

Directory of Open Access Journals (Sweden)

M. J. A. Werger

1983-11-01

Full Text Available Southern Africa is characterized by a highly diversified vegetational cover with extremes as contrasting as desert, tropical forest, alpine grassland, or mediterranean type scrub, and many other types in between. This vegetational pattern is strongly correlated to the climatological pattern. It is therefore likely that past changes in climate can still be partly traced in the vegetational pattern, particularly in geographical anomalies, and that study of these patterns provides complementary evidence to palynological research. The following anomalies in the vegetational pattern are briefly discussed: 1. island-wise occurrence of Afro-montane vegetation on mesic, sheltered sites in the southern Sudano- Zambezian Region, in particular in the Highveld grassland/False Karoo transition area; 2. similar westward occurrence of Sudano-Zambezian scrub patches in the Karoo-Namib Region near the Orange/Vaal confluence; 3. scattered occurrence of Sudano-Zambezian woody species in a matrix of Karoo-Namib vegetation in the marginal Karoo-Namib Region; 4. island-wise occurrence of frost-tolerant, dry, karroid dwarf shrub vegetation of predominantly C,-plants on isolated peaks in the winter rainfall area of Namaqualand; 5. peculiar patchy distribution of some succulents in wide areas of climatically rather homogeneous, succulent dwarf shrub vegetation of predominantly CAM-plants in the escarpment area of Namaqualand. a pattern reminiscent of that in many Cape fynbos species. Interpretation of these patterns logically leads to the conclusion that these result from a previously wetter, a previously cooler, or a previously wetter and cooler climate, respectively, over the parts of southern Africa under discussion. This conclusion is compared with published palynological views.

Desinfestação de substratos com a utilização de coletor solar Utilization of solar collector for treatment of plant growth substrates

Directory of Open Access Journals (Sweden)

Raquel Ghini

1992-01-01

Full Text Available Coletores solares planos constituídos de caixas de madeira com canaletas de chapa de alumínio, onde se coloca o substrato e se cobre com plástico transparente, foram testados quanto ao controle de Sclerotium rolfsii, Rhizoctonia solani, Verticillium sp., Meloidogyne arenaria e Cyperus rotundus (tiririca. Dependendo da intensidade de radiação solar, é necessário um dia para desinfestação do substrato com S. rolfsii e dois dias para R. solani, Verticillium sp. e M. arenaria.Flat solar collectors were tested for the control of Sclerotium rolfsii, Rhizoctonia solani, VerticiUium sp., Meloidogyne arenaria and Cyperus rotundus (nut sedge. The equipment developed comprises, basically, gutters of aluminum with termic liner of glass wool and transparent plastic cover. The results showed that, depending upon the solar radiation, one day is required for the disinfestations of substrate infested with S. rolfsii and nut sedge, and two days for R. solani, Verticillium sp. and M. arenaria.

Biodegradation of14 C-lindane by some soil fungi

International Nuclear Information System (INIS)

Attaby, H.S.H.; Adam, Y.M.

1991-01-01

In a culture medium incorporated with 14 C-labelled lindane, the 3 soil fungi helminthosporium sp., alternaria brassicola, and verticillium agaricinum were incubated for 10 days. Most of the recovered radioactivity was found to be maintained mainly in the medium of helminthosporium and alternaria but in case of verticillium the majority was associated with the mat. The 3 fungal species showed degradation capacity of 6.1, 5.1 and 3.2% of the total recovered radioactivity radioactivity as hydrolytic products. TLC chromatographic analysis of chloroform fractions revealed the presence of only unchanged lindane either in medium of mat. The aqueous fractions of the 3 fungal species, demonstrated three degraded products (unknown ι,π and tri) having Rf values of 0.40 - 0.47, 0.66 - 0.73, and 0.87 in addition to a polar compound (Rf = 0.07) with the major radioactivity. A certain percentage of radiocarbon 6.7, 5.7 and 30.3% of the mats of helminthosporium, alternria and verticillium, respectively.2 tab

Australian consumer awareness of health benefits associated with vegetable consumption.

Science.gov (United States)

Rekhy, Reetica; Khan, Aila; Eason, Jocelyn; Mactavish-West, Hazel; Lister, Carolyn; Mcconchie, Robyn

2017-04-01

The present study investigated the perceived health benefits of specific vegetable consumption to guide the use of nutrition and health claims on vegetable marketing collateral. Free elicitation and consumer ranking data were collected through an online survey of 1000 adults from across Australia and analysed for the perceived importance of vegetables in the daily diet, number of serves consumed per day, knowledge about health-related benefits of specific vegetables and perceived health benefits of vegetable consumption. The importance of vegetables in the diet and daily vegetable consumption was higher in people from an English-speaking background, females, people aged 45 years and over and people living in non-metropolitan areas. Digestion was selected as the major health benefit from consumption of specific vegetables. However, understanding of the health benefits of specific vegetable consumption was relatively low among consumers. Half of the respondents were not sure of the health benefits associated with specific vegetables, except for carrots and spinach. Some respondents volunteered nutrient content or other information. There was no clear indication that consumers understand the specific health benefits conferred by consumption of vegetables. Nutrient and health benefit labelling therefore has the capacity to enhance knowledge of vegetable consumers. It is recommended that health benefit labelling be tailored to promote greater consumption of vegetables in those demographic groups where vegetable consumption was lower. The present study assists the Australian vegetable industry in helping consumers make more informed consumption choices. © 2016 Dietitians Association of Australia.

Identification of Distribution Channels to Create Sustainable Vegetable Prices

Directory of Open Access Journals (Sweden)

Aflit Nuryulia Praswati

2017-12-01

Full Text Available The price of vegetables has a role as a contributor to the rate of inflation. Currently the number of vegetable production in Boyolali region can no longer meet the needs of local communities. The limited amount of vegetable production and the inhibition of the vegetable distribution channel creates a scarcity of vegetables that result in price increases. This study aims to identify the distribution channel and the formation of vegetable prices derived from Boyolali area. The method used in this research is quantitative and qualitative. Respondents from this study consisted of farmers, wholesalers, small trader and end consumers. The type of distribution channel prevailing in Boyolali area are traditional and modern distribution channels. Intermediate distribution channels play a greater role in determining vegetable prices. If farmers want to improve their economic condition, it needs innovation and creativity in the process of planting, harvesting, packaging and marketing vegetable products.

Effects of repeated exposure to either vegetables or fruits on infant's vegetable and fruit acceptance at the beginning of weaning

NARCIS (Netherlands)

Barends, C.; Vries, de J.; Mojet, J.; Graaf, de C.

2013-01-01

This study investigated the effects of repeated exposure to either vegetables or fruits on an infant's vegetable and fruit acceptance during the first 18 days of weaning. We hypothesized that repeated exposure to a type of vegetable or fruit, would increase its intake. Furthermore, we expected that

A morphometric analysis of vegetation patterns in dryland ecosystems

Science.gov (United States)

Mander, Luke; Dekker, Stefan C.; Li, Mao; Mio, Washington; Punyasena, Surangi W.; Lenton, Timothy M.

2017-02-01

Vegetation in dryland ecosystems often forms remarkable spatial patterns. These range from regular bands of vegetation alternating with bare ground, to vegetated spots and labyrinths, to regular gaps of bare ground within an otherwise continuous expanse of vegetation. It has been suggested that spotted vegetation patterns could indicate that collapse into a bare ground state is imminent, and the morphology of spatial vegetation patterns, therefore, represents a potentially valuable source of information on the proximity of regime shifts in dryland ecosystems. In this paper, we have developed quantitative methods to characterize the morphology of spatial patterns in dryland vegetation. Our approach is based on algorithmic techniques that have been used to classify pollen grains on the basis of textural patterning, and involves constructing feature vectors to quantify the shapes formed by vegetation patterns. We have analysed images of patterned vegetation produced by a computational model and a small set of satellite images from South Kordofan (South Sudan), which illustrates that our methods are applicable to both simulated and real-world data. Our approach provides a means of quantifying patterns that are frequently described using qualitative terminology, and could be used to classify vegetation patterns in large-scale satellite surveys of dryland ecosystems.

Parasitic Contamination of Raw Vegetables in Zanjan Markets, Iran

Directory of Open Access Journals (Sweden)

Negin Torabi

2016-09-01

Full Text Available Background: Complex surface of vegetables facilitate attachment and transmission of several pathogens. No previous study has been conducted in survey of parasitic contamination of vegetables in Zanjan. This study aimed to detect the parasitic contamination in common raw vegetables in Zanjan markets. Methods: A total of 352 raw vegetable samples, including leek, parsley, basil, mint, radish, cress and dill were collected from grocery stores using cluster sampling in different regions of the city during 2014. The edible parts of vegetables were separated and immersed in normal saline solution. Floating vegetables were removed and the solution was allowed to sediment at room temperature for 24 hours. The pellet was examined following sedimentation and floatation methods. Results:Various Organisms were detected in 54% (190 of the 352 samples, but only 2.8% of samples had pathogenic parasites including; Trichostrongylus eggs (3, Hookworm eggs (2, Eimeria oocysts (2, Sarcocystis oocyst (1, Strongyloides larvae (1, and Fasciola eggs (1. The contamination rate of vegetables was highest (90.4% in the fall (p˂0.05. Conclusion: Vegetable contamination with parasitic organisms in this area was low, maybe due to irrigation of vegetables with sources other than sewage water, but it is still necessary to improve sanitary conditions of vegetables.

Farming of Vegetables in Space-Limited Environments

Science.gov (United States)

He, Jie

2015-10-01

Vegetables that contain most of the essential components of human nutrition are perishable and cannot be stocked. To secure vegetable supply in space limited cities such as Singapore, there are different farming methods to produce vegetables. These include low-cost urban community gardening and innovative rooftop and vertical farms integrated with various technologies such as hydroponics, aquaponics and aeroponics. However, for large-scale vegetable production in space-limited Singapore, we need to develop farming systems that not only increase productivity many-fold per unit of land but also produce all types of vegetable, all year-round for today and the future. This could be resolved through integrated vertical aeroponic farming system. Manipulation of root-zone (RZ) environments such as cooling the RZ, modifying mineral nutrients and introducing elevated RZ CO2 using aeroponics can further boost crop productivity beyond what can be achieved from more efficient use of land area. We could also adopt energy saving light emitting diodes (LEDs) for vertical aeroponic farming system to promote uniform growth and to improve the utilisation of limited space via shortening the growth cycle, thus improving vegetable production in a cost-effective manner.

Curtimento de peles de peixe com taninos vegetal e sintético = Tanning fish skins using vegetable and synthetic tannins

Directory of Open Access Journals (Sweden)

Ariana Martins Vieira

2008-07-01

Full Text Available No presente experimento objetivou-se avaliar a resistência das peles de peixes submetidas ao curtimento com taninos vegetal e sintético. As peles foram distribuídas em um delineamento inteiramente casualizado, com seis tratamentos aplicados na etapa decurtimento (T1 = 10% tanino vegetal; T2=10% tanino sintético; T3 = 5% tanino vegetal + 5% sintético; T4 = 12% tanino vegetal; T5 = 12% tanino sintético; T6 = 6% tanino vegetal + 6% sintético e dez repetições por tratamento, sendo o couro a unidade experimental.Para os testes de resistência à tração, alongamento e rasgamento, foi utilizado o dinamômetro EMIC. O curtimento com tanino vegetal, sintético e/ou sua combinação, assim como os níveis utilizados dentro de cada técnica de curtimento, interferiu no teste de tração, no entanto não influenciou No teste de alongamento e rasgamento progressivo. Portanto, a técnica de curtimento interfere na resistência dos couros de peixes. As peles de tilápia, quando curtidas e recurtidas apenas com tanino sintético, independentemente da concentração (10 ou 12%, apresentaram pior resistência à tração. Quando comparados com os que receberam 10% de taninos (vegetal e a combinação dos taninos, esses couros não apresentaram diferença na resistência.The objective of the present experiment was to evaluate the resistance of fish skins submitted to tanning using vegetable and synthetic tannins. Skins were distributed in a completelyrandomized design, with six treatments applied in the tanning stage (T1 = 10% vegetable tannin; T2 = 10% synthetic tannin; T3 = 5% vegetable tannin + 5% synthetic tannin; T4 = 12% vegetable tannin; T5 = 12% synthetic tannin; T6 = 6% vegetable tannin + 6% synthetic. Ten repetitions per treatment were conducted, and the leather was theexperimental unit. For the tests of tensile strength, lengthening and progressive tearing resistance, an EMIC dynamometer was used. Tanning with vegetable tannin, synthetic

Nutritional potentials of some tropical vegetable leaf meals ...

African Journals Online (AJOL)

Three tropical vegetable species (Talium triangulare, Amaranthus cruentus and Telfairia occidentalis) were selected based on their availability and agronomic desirability. The freshly harvested vegetable leaves were subjected to 2 processing techniques (shredding and sundrying) before milling into the vegetable leaf ...

Terrestrial transect study on driving mechanism of vegetation changes

Institute of Scientific and Technical Information of China (English)

2008-01-01

In terms of Chinese climate-vegetation model based on the classification of plant functional types, to- gether with climatic data from 1951 to 1980 and two future climatic scenarios (SRES-A2 and SRES-B2) in China from the highest and the lowest emission scenarios of greenhouse gases, the distribution patterns of vegetation types and their changes along the Northeast China Transect (NECT) and the North-South Transect of Eastern China (NSTEC) were simulated in order to understand the driving mechanisms of vegetation changes under climatic change. The results indicated that the vegetation distribution patterns would change significantly under future climate, and the major factors driving the vegetation changes were water and heat. However, the responses of various vegetation types to the changes in water and heat factors were obviously different. The vegetation changes were more sensi- tive to heat factors than to water factors. Thus, in the future climate warming will significantly affect vegetation distribution patterns.

Modelling post-fire vegetation recovery in Portugal

Science.gov (United States)

Bastos, A.; Gouveia, C. M.; Dacamara, C. C.; Trigo, R. M.

2011-12-01

Wildfires in Mediterranean Europe have been increasing in number and extension over the last decades and constitute one of the major disturbances of these ecosystems. Portugal is the country with more burnt area in the last decade and the years of 2003 and 2005 were particularly devastating, the total burned areas of 425 000 and 338 000 ha being several times higher than the corresponding average. The year of 2005 further coincided with one of the most severe droughts since early 20th century. Due to different responses of vegetation to diverse fire regimes and to the complexity of landscape structures, fires have complex effects on vegetation recovery. Remote sensing has revealed to be a powerful tool in studying vegetation dynamics and in monitoring post-fire vegetation recovery, which is crucial to land-management and to prevent erosion. The main goals of the present work are (i) to assess the accuracy of a vegetation recovery model previously developed by the authors; (ii) to assess the model's performance, namely its sensitivity to initial conditions, to the temporal length of the input dataset and to missing data; (iii) to study vegetation recovery over two selected areas that were affected by two large wildfire events in the fire seasons of 2003 and 2005, respectively. The study relies on monthly values of NDVI over 11 years (1998-2009), at 1 km × 1 km spatial resolution, as obtained by the VEGETATION instrument. According to results from sensitivity analysis, the model is robust and able to provide good estimations of recovery times of vegetation when the regeneration process is regular, even when missing data is present. In respect to the two selected burnt scars, results indicate that fire damage is a determinant factor of regeneration, as less damaged vegetation recovers more rapidly, which is mainly justified by the high coverage of Pinus pinaster over the area, and by the fact that coniferous forests tend to recover slower than transitional woodland

Projected future vegetation changes for the northwest United States and southwest Canada at a fine spatial resolution using a dynamic global vegetation model.

Science.gov (United States)

Shafer, Sarah; Bartlein, Patrick J.; Gray, Elizabeth M.; Pelltier, Richard T.

2015-01-01

Future climate change may significantly alter the distributions of many plant taxa. The effects of climate change may be particularly large in mountainous regions where climate can vary significantly with elevation. Understanding potential future vegetation changes in these regions requires methods that can resolve vegetation responses to climate change at fine spatial resolutions. We used LPJ, a dynamic global vegetation model, to assess potential future vegetation changes for a large topographically complex area of the northwest United States and southwest Canada (38.0–58.0°N latitude by 136.6–103.0°W longitude). LPJ is a process-based vegetation model that mechanistically simulates the effect of changing climate and atmospheric CO2 concentrations on vegetation. It was developed and has been mostly applied at spatial resolutions of 10-minutes or coarser. In this study, we used LPJ at a 30-second (~1-km) spatial resolution to simulate potential vegetation changes for 2070–2099. LPJ was run using downscaled future climate simulations from five coupled atmosphere-ocean general circulation models (CCSM3, CGCM3.1(T47), GISS-ER, MIROC3.2(medres), UKMO-HadCM3) produced using the A2 greenhouse gases emissions scenario. Under projected future climate and atmospheric CO2 concentrations, the simulated vegetation changes result in the contraction of alpine, shrub-steppe, and xeric shrub vegetation across the study area and the expansion of woodland and forest vegetation. Large areas of maritime cool forest and cold forest are simulated to persist under projected future conditions. The fine spatial-scale vegetation simulations resolve patterns of vegetation change that are not visible at coarser resolutions and these fine-scale patterns are particularly important for understanding potential future vegetation changes in topographically complex areas.

Exploiting differential vegetation phenology for satellite-based mapping of semiarid grass vegetation in the southwestern United States and northern Mexico

Science.gov (United States)

Dye, Dennis G.; Middleton, Barry R.; Vogel, John M.; Wu, Zhuoting; Velasco, Miguel G.

2016-01-01

We developed and evaluated a methodology for subpixel discrimination and large-area mapping of the perennial warm-season (C4) grass component of vegetation cover in mixed-composition landscapes of the southwestern United States and northern Mexico. We describe the methodology within a general, conceptual framework that we identify as the differential vegetation phenology (DVP) paradigm. We introduce a DVP index, the Normalized Difference Phenometric Index (NDPI) that provides vegetation type-specific information at the subpixel scale by exploiting differential patterns of vegetation phenology detectable in time-series spectral vegetation index (VI) data from multispectral land imagers. We used modified soil-adjusted vegetation index (MSAVI2) data from Landsat to develop the NDPI, and MSAVI2 data from MODIS to compare its performance relative to one alternate DVP metric (difference of spring average MSAVI2 and summer maximum MSAVI2), and two simple, conventional VI metrics (summer average MSAVI2, summer maximum MSAVI2). The NDPI in a scaled form (NDPIs) performed best in predicting variation in perennial C4 grass cover as estimated from landscape photographs at 92 sites (R2 = 0.76, p landscapes of the Southwest, and potentially for monitoring of its response to drought, climate change, grazing and other factors, including land management. With appropriate adjustments, the method could potentially be used for subpixel discrimination and mapping of grass or other vegetation types in other regions where the vegetation components of the landscape exhibit contrasting seasonal patterns of phenology.

Suppression of vegetation in LANDSAT ETM+ remote sensing images

Science.gov (United States)

Yu, Le; Porwal, Alok; Holden, Eun-Jung; Dentith, Michael

2010-05-01

Vegetation cover is an impediment to the interpretation of multispectral remote sensing images for geological applications, especially in densely vegetated terrains. In order to enhance the underlying geological information in such terrains, it is desirable to suppress the reflectance component of vegetation. One form of spectral unmixing that has been successfully used for vegetation reflectance suppression in multispectral images is called "forced invariance". It is based on segregating components of the reflectance spectrum that are invariant with respect to a specific spectral index such as the NDVI. The forced invariance method uses algorithms such as software defoliation. However, the outputs of software defoliation are single channel data, which are not amenable to geological interpretations. Crippen and Blom (2001) proposed a new forced invariance algorithm that utilizes band statistics, rather than band ratios. The authors demonstrated the effectiveness of their algorithms on a LANDSAT TM scene from Nevada, USA, especially in open canopy areas in mixed and semi-arid terrains. In this presentation, we report the results of our experimentation with this algorithm on a densely to sparsely vegetated Landsat ETM+ scene. We selected a scene (Path 119, Row 39) acquired on 18th July, 2004. Two study areas located around the city of Hangzhou, eastern China were tested. One of them covers uninhabited hilly terrain characterized by low rugged topography, parts of the hills are densely vegetated; another one covers both inhabited urban areas and uninhabited hilly terrain, which is densely vegetated. Crippen and Blom's algorithm is implemented in the following sequential steps: (1) dark pixel correction; (2) vegetation index calculation; (3) estimation of statistical relationship between vegetation index and digital number (DN) values for each band; (4) calculation of a smooth best-fit curve for the above relationships; and finally, (5) selection of a target average DN

Tundra vegetation effects on pan-Arctic albedo

International Nuclear Information System (INIS)

Loranty, Michael M; Goetz, Scott J; Beck, Pieter S A

2011-01-01

Recent field experiments in tundra ecosystems describe how increased shrub cover reduces winter albedo, and how subsequent changes in surface net radiation lead to altered rates of snowmelt. These findings imply that tundra vegetation change will alter regional energy budgets, but to date the effects have not been documented at regional or greater scales. Using satellite observations and a pan-Arctic vegetation map, we examined the effects of shrub vegetation on albedo across the terrestrial Arctic. We included vegetation classes dominated by low shrubs, dwarf shrubs, tussock-dominated graminoid tundra, and non-tussock graminoid tundra. Each class was further stratified by bioclimate subzones. Low-shrub tundra had higher normalized difference vegetation index values and earlier albedo decline in spring than dwarf-shrub tundra, but for tussock tundra, spring albedo declined earlier than for low-shrub tundra. Our results illustrate how relatively small changes in vegetation properties result in differences in albedo dynamics, regardless of shrub growth, that may lead to differences in net radiation upwards of 50 W m -2 at weekly time scales. Further, our findings imply that changes to the terrestrial Arctic energy budget during this important seasonal transition are under way regardless of whether recent satellite observed productivity trends are the result of shrub expansion. We conclude that a better understanding of changes in vegetation productivity and distribution in Arctic tundra is essential for accurately quantifying and predicting carbon and energy fluxes and associated climate feedbacks.

Vegetative buffer strips for reducing herbicide transport in runoff: effects of buffer width, vegetation, and season

Science.gov (United States)

The effect of vegetative buffer strip (VBS) width, vegetation, and season of the year on herbicide transport in runoff has not been well documented for runoff prone soils. A multi-year replicated plot-scale study was conducted on an eroded claypan soil with the following objectives: 1) assess the ef...

The technology of fish-vegetable feed production

Directory of Open Access Journals (Sweden)

Mukatova M. D.

2016-09-01

Full Text Available Perspective direction of the Volga-Caspian basin fisheries is increasing the productivity of aquaculture production which requires the availability of sufficient quantities of feed. The cutting waste of carp and crucian carp, crayfish processing (cephalothorax, wheat bran, soy isolate, freshwater plants – pondweed perfoliate, fish-vegetable ration, produced feeding staffs have been investigated. In researching samples of manufactured pelleted feeds the standard methods adopted in the animal feed industry have been used. The number of nitrogen-free extractives and energy value has been determined by calculation. The composition of fish-vegetable ration has been worked out. Some manufacturing inspection of fish-vegetable feed technology using proofing process has been carried out. The possibility of manufacturing on the basis of crushed fish waste of the company LLC "VES" and dry ingredients of fish-vegetable feed has been determined; the output of feed at water content of not more than 10 % is 43 % of feed mix based on the mass of directed waste equal to 84 %. The pilot batch of dry fish-vegetable feed has been investigated to establish quality indicators. It has been determined that fish-vegetable feed meets the requirements of GOST 10385–2014 "Combined feeding staffs for fishes. General specifications" as for main quality indicators and refers to economic grower for catfish and carp fish weighing more than 50 g. This reveals good palatability of the experimental batch of floating feed by carp fish species and African catfish. Thus, fish-vegetable feed manufacturing technology can be implemented in the production for processing secondary raw materials: waste from butchering fish by grinding, cooking, mixing with selected vegetable fillings which is waste of flour or grain processing industries and freshwater plants mowed annually during the reclamation works on the Volga delta.

Man-influenced vegetation of North Korea

Czech Academy of Sciences Publication Activity Database

Kolbek, Jiří; Jarolímek, I.

2008-01-01

Roč. 40, č. 1 (2008), s. 381-404 ISSN 0253-116X R&D Projects: GA ČR(CZ) GA206/05/0119 Institutional research plan: CEZ:AV0Z60050516 Keywords : vegetation classification * weed communities * man-depending vegetation Subject RIV: EF - Botanics

Natural Vegetation of the Flora area

DEFF Research Database (Denmark)

Sebsebe, Demissew; Friis, Ib

2009-01-01

A review article summarising the recent ideas about the natural vegetation in the area covered by the Flora of Ethiopia and Eritrea......A review article summarising the recent ideas about the natural vegetation in the area covered by the Flora of Ethiopia and Eritrea...

Serving large portions of vegetable soup at the start of a meal affected children's energy and vegetable intake.

Science.gov (United States)

Spill, Maureen K; Birch, Leann L; Roe, Liane S; Rolls, Barbara J

2011-08-01

This study tested whether varying the portion of low-energy-dense vegetable soup served at the start of a meal affects meal energy and vegetable intakes in children. Subjects were 3- to 5-year-olds (31 boys and 41 girls) in daycare facilities. Using a crossover design, children were served lunch once a week for four weeks. On three occasions, different portions of tomato soup (150, 225, and 300 g) were served at the start of the meal, and on one occasion no soup was served. Children had 10 min to consume the soup before being served the main course. All foods were consumed ad libitum. The primary outcomes were soup intake as well as energy and vegetable intake at the main course. A mixed linear model tested the effect of soup portion size on intake. Serving any portion of soup reduced entrée energy intake compared with serving no soup, but total meal energy intake was only reduced when 150 g of soup was served. Increasing the portion size increased soup and vegetable intake. Serving low-energy-dense, vegetable soup as a first course is an effective strategy to reduce children's intake of a more energy-dense main entrée and increase vegetable consumption at the meal. Copyright © 2011 Elsevier Ltd. All rights reserved.

Forest Vegetation Simulator translocation techniques with the Bureau of Land Management's Forest Vegetation Information system database

Science.gov (United States)

Timothy A. Bottomley

2008-01-01

The BLM uses a database, called the Forest Vegetation Information System (FORVIS), to store, retrieve, and analyze forest resource information on a majority of their forested lands. FORVIS also has the capability of easily transferring appropriate data electronically into Forest Vegetation Simulator (FVS) for simulation runs. Only minor additional data inputs or...

Symptoms of main Callistephus chinensis L. Nees. diseases under Ukrainian urban ecosystem conditions of the forest-steppe zone

Directory of Open Access Journals (Sweden)

Marchenko Alla

2017-12-01

Full Text Available Phytopathological monitoring of C. chinensis (L. Nees. has proven withering and root rot to be the dominating diseases in agrobiocenoses under Ukrainian urban ecosystem conditions of the forest-steppe zone. Their spread was 5,1 and 4 times more than one of spotting. The complex of plant pathogenic overground and underground microflora consists of 24 causative agents. B. cinerea, F. oxysporum, V. albo-atrum have been found on all the vegetative and reproductive parts of Callistephus chinensis (L. Nees., , Ph. cactorum – on plant overground and underground parts, Rh. solani – on underground parts and seeds, A. zinniae – on overground parts and seeds. The main C. chinensis (L. Nees. disease symptoms have been diagnosed (leaf spots, powdery mildew, verticillium wilt, rust, ramularia spot, septoria spots, botrytis blight, grey mold rot, late blight, fusarium blight, black stem.

Plant functional diversity affects climate-vegetation interaction

Science.gov (United States)

Groner, Vivienne P.; Raddatz, Thomas; Reick, Christian H.; Claussen, Martin

2018-04-01

We present how variations in plant functional diversity affect climate-vegetation interaction towards the end of the African Humid Period (AHP) in coupled land-atmosphere simulations using the Max Planck Institute Earth system model (MPI-ESM). In experiments with AHP boundary conditions, the extent of the green Sahara varies considerably with changes in plant functional diversity. Differences in vegetation cover extent and plant functional type (PFT) composition translate into significantly different land surface parameters, water cycling, and surface energy budgets. These changes have not only regional consequences but considerably alter large-scale atmospheric circulation patterns and the position of the tropical rain belt. Towards the end of the AHP, simulations with the standard PFT set in MPI-ESM depict a gradual decrease of precipitation and vegetation cover over time, while simulations with modified PFT composition show either a sharp decline of both variables or an even slower retreat. Thus, not the quantitative but the qualitative PFT composition determines climate-vegetation interaction and the climate-vegetation system response to external forcing. The sensitivity of simulated system states to changes in PFT composition raises the question how realistically Earth system models can actually represent climate-vegetation interaction, considering the poor representation of plant diversity in the current generation of land surface models.

Fruits, vegetables, 100% juices, and cognitive function.

Science.gov (United States)

Lamport, Daniel J; Saunders, Caroline; Butler, Laurie T; Spencer, Jeremy Pe

2014-12-01

Although reviews of the association between polyphenol intake and cognition exist, research examining the cognitive effects of fruit, vegetable, and juice consumption across epidemiological and intervention studies has not been previously examined. For the present review, critical inclusion criteria were human participants, a measure of fruit, vegetable, or 100% juice consumption, an objective measure of cognitive function, and a clinical diagnosis of neuropsychological disease. Studies were excluded if consumption of fruits, vegetables, or juice was not assessed in isolation from other food groups, or if there was no statistical control for education or IQ. Seventeen of 19 epidemiological studies and 3 of 6 intervention studies reported significant benefits of fruit, vegetable, or juice consumption for cognitive performance. The data suggest that chronic consumption of fruits, vegetables, and juices is beneficial for cognition in healthy older adults. The limited data from acute interventions indicate that consumption of fruit juices can have immediate benefits for memory function in adults with mild cognitive impairment; however, as of yet, acute benefits have not been observed in healthy adults. Conclusions regarding an optimum dietary intake for fruits, vegetables, and juices are difficult to quantify because of substantial heterogeneity in the categorization of consumption of these foods. © 2014 International Life Sciences Institute.

Fruit and vegetable consumption: benefits and barriers.

Science.gov (United States)

Maclellan, Debbie L; Gottschall-Pass, Katherine; Larsen, Roberta

2004-01-01

Few people on Prince Edward Island meet the goal of consuming five or more servings of vegetables and fruit a day. The main objective of this qualitative study was to explore the perceptions of the nutritional benefits and barriers to vegetable and fruit intake among adult women in Prince Edward Island. Participants were 40 women aged 20-49, with or without children at home, who were or were not currently meeting the objective of eating five or more fruit and vegetable servings a day. In-home, one-on-one interviews were used for data collection. Thematic analysis was conducted on the transcribed interviews. Data were examined for trustworthiness in the context of credibility, transferability, and dependability. Most participants identified one or more benefits of eating fruit and vegetables; however, comments tended to be non-specific. The main barriers that participants identified were effort, lack of knowledge, sociopsychological and socioenvironmental factors, and availability. Internal influences, life events, and food rules were identified as encouraging women to include vegetables and fruit in their diets. Given the challenges of effecting meaningful dietary change, dietitians must look for broader dietary behavioural interventions that are sensitive to women's perceptions of benefits and barriers to fruit and vegetable intake.

Vegetation

DEFF Research Database (Denmark)

Epstein, H.E.; Walker, D.A.; Bhatt, U.S.

2012-01-01

increased 20-26%. • Increasing shrub growth and range extension throughout the Low Arctic are related to winter and early growing season temperature increases. Growth of other tundra plant types, including graminoids and forbs, is increasing, while growth of mosses and lichens is decreasing. • Increases...... in vegetation (including shrub tundra expansion) and thunderstorm activity, each a result of Arctic warming, have created conditions that favor a more active Arctic fire regime....

Assessment of human impacts on landuse and vegetation cover ...

African Journals Online (AJOL)

This paper is an assessment of the impact of man's activities on the landuse and vegetation cover of Mubi region. Landsat MSS Landuse/vegetation image of 1978 and Spot XS landuse/vegetation image of 1995 were used to study the landuse/vegetation cover changes of the region between 1978 and 1995 – a period of 17 ...

Conveyance estimation in channels with emergent bank vegetation ...

African Journals Online (AJOL)

Emergent vegetation along the banks of a river channel influences its conveyance considerably. The total channel discharge can be estimated as the sum of the discharges of the vegetated and clear channel zones calculated separately. The vegetated zone discharge is often negligible, but can be estimated using ...

Utility of Rice Bran Mixed with Fermentation Extract of Vegetable Waste Unconditioned as Probiotics from Vegetable Market

Directory of Open Access Journals (Sweden)

Cahya Setya Utama

2013-06-01

Full Text Available The aimed of the study was to enhance utilization of Lactobacillus Sp. and Saccharomyces sp. as starter for fermentation of waste cabbage and green mustard to enhance the utility of rice bran as an ingredient of poultry feed. The first stage of the study was to characterize the extract of fermented wasted vegetable with difference concentration of glucose and different period of time for incubation. Completely randomized design using 3 x 3 factorial with three replications was used for the first stage of the study. The second stage of the study was to evaluate the effectiveness of the extract of fermented wasted vegetable to improve the quality and utility of rice bran. Completely randomized design with four treatments and 4 replications. The third stage of the study was to obtain the value of nitrogen, calcium and phosphorus retention, as well as metabolic energy of rice bran after fermentation in broiler hatching. The third stage analysis was conducted by t-test analysis. The results of the study showed that the microbial characteristics on the extract of fermented wasted vegetable in two days incubation time and 2% concentration of glucose was able to produce pH of 3.80, log of total lactic acid bacteria 7.38 and types of microbes that grow predominantly were Lactobacillus Sp. and Saccharomyces Sp. The effectiveness of the extract fermented wasted vegetable for the fermentation of rice bran were shown in additional concentration of extract wasted vegetable of 40% with a moisture content of  toluene 65.24%, 13.36% ash, 14.93% protein, 7.61% crude fat, 12.39% crude fiber, nitrogen free extract material (NFE 51.59% and 0.07% biomass. The biological value and the availability of fermented rice bran were higher than unfermented rice bran on the broiler chickens after hatching. The values were justified from nitrogen, calcium and phosphorus retention also metabolic energy. The study concluded that the benefits and utility of rice bran could

Serving large portions of vegetable soup at the start of a meal affected children’s energy and vegetable intake

Science.gov (United States)

Spill, Maureen K.; Birch, Leann L.; Roe, Liane S.; Rolls, Barbara J.

2011-01-01

This study tested whether varying the portion of low-energy-dense vegetable soup served at the start of a meal affects meal energy and vegetable intakes in children. Subjects were 3- to 5-year-olds (31 boys and 41 girls) in daycare facilities. Using a crossover design, children were served lunch once a week for four weeks. On three occasions, different portions of tomato soup (150, 225, and 300 g) were served at the start of the meal, and on one occasion no soup was served. Children had 10 minutes to consume the soup before being served the main course. All foods were consumed ad libitum. The primary outcomes were soup intake as well as energy and vegetable intake at the main course. A mixed linear model tested the effect of soup portion size on intake. Serving any portion of soup reduced entrée energy intake compared with serving no soup, but total meal energy intake was only reduced when 150 g of soup was served. Increasing the portion size increased soup and vegetable intake. Serving low-energy-dense, vegetable soup as a first course is an effective strategy to reduce children’s intake of a more energy-dense main entrée and increase vegetable consumption at the meal. PMID:21596073

Water quality function of an extensive vegetated roof.

Science.gov (United States)

Todorov, Dimitar; Driscoll, Charles T; Todorova, Svetoslava; Montesdeoca, Mario

2018-06-01

In this paper we present the results of a four-year study of water quality in runoff from an extensive, sedum covered, vegetated roof on an urban commercial building. Monitoring commenced seven months after the roof was constructed, with the first growing season. Stormwater drainage quality function of the vegetated roof was compared to a conventional (impermeable, high-albedo) membrane roof in addition to paired measurements of wet and bulk depositions at the study site. We present concentrations and fluxes of nutrients and major solutes. We discuss seasonal and year-to-year variation in water quality of drainage from the vegetated roof and how it compares with atmospheric deposition and drainage from the impermeable roof. Drainage waters from the vegetated roof exhibited a high concentration of nutrients compared to atmospheric deposition, particularly during the warm temperature growing season. However, nutrient losses were generally low because of the strong retention of water by the vegetated roof. There was marked variation in the retention of nutrients by season due to variations in concentrations in drainage from the vegetated roof. The vegetated roof was a sink of nitrogen, total phosphorus and chloride, and a source of phosphate and dissolved inorganic and organic carbon. Chloride exhibited elevated inputs and leaching during the winter. The drainage from the vegetated and impermeable roofs met the United States Environmental Protection Agency freshwater standards for all parameters, except for total phosphorus. Copyright © 2017 Elsevier B.V. All rights reserved.

Listeria monocytogenes - Danger for health safety vegetable production.

Science.gov (United States)

Kljujev, Igor; Raicevic, Vera; Jovicic-Petrovic, Jelena; Vujovic, Bojana; Mirkovic, Milica; Rothballer, Michael

2018-04-22

The microbiologically contaminated vegetables represent a risk for consumers, especially vegetables without thermal processing. It is known that human pathogen bacteria, such as Listeria monocytogenes, could exist on fresh vegetables. The fresh vegetables could become Listeria-contaminated if they come in touch with contaminated soil, manure, irrigation water. The aim of this work was to investigate the presence of Listeria spp. and L. monocytogenes in different kind of vegetables grown in field and greenhouse condition as well as surface and endophytic colonization plant roots of different vegetables species by L. monocytogenes in laboratory conditions. The detection of Listeria spp. and L. monocytogenes in vegetable samples was done using ISO and PCR methods. The investigation of colonization vegetable roots and detection Listeria-cells inside plant root tissue was done using Fluorescence in situ hybridization (FISH) method in combination with confocal laser scanning microscopy (CLSM). The results showed that 25.58% vegetable samples were positive for Listeria spp. and only one sample (carrot) was positive for L. monocytogenes out of 43 samples in total collected from field and greenhouse. The strain L. monocytogenes EGD-E surface and endophytic colonized carrot root in highest degree while strain L. monocytogenes SV4B was the most represented at leafy vegetable plants, such at lettuce (1.68 × 10 6  cells/mm 3 absolutely dry root) and spinach (1.39 × 10 6  cells/mm 3 absolutely dry root) root surface. The cells of L. monocytogenes SV4B were visible as single cells in interior tissue of plant roots (celery and sweet corn roots) as well as in the interior of the plant root cell at sweet corn root. The cells of L. monocytogenes EGD-E bind to the surface of the plant root and they were less commonly found out on root hair. In the inner layers of the root, those bacterial cells were inhabited intercellular spaces mainly as single cells very close to the

24 GHz cmWave Radio Propagation Through Vegetation

DEFF Research Database (Denmark)

Rodriguez, Ignacio; Abreu, Renato Barbosa; Portela Lopes de Almeida, Erika

2016-01-01

This paper presents a measurement-based analysis of cm-wave radio propagation through vegetation at 24 GHz. A set of dedicated directional measurements were performed with horn antennas located close to street level inside a densely-vegetated area illuminated from above. The full azimuth was exam......This paper presents a measurement-based analysis of cm-wave radio propagation through vegetation at 24 GHz. A set of dedicated directional measurements were performed with horn antennas located close to street level inside a densely-vegetated area illuminated from above. The full azimuth...

Investigation of NPK in fertilized and unfertilized vegetables

International Nuclear Information System (INIS)

Deeba, F.; Butt, M.T.; Iqubal, K.; Shafiq, T.

2010-01-01

Contents of selected minerals and moisture in Ridge Gourd, Gourd and Brinjal vegetables, collected from house and market of different localities, were determined. In house vegetables from kitchen garden of PCSIR colony the moisture contents were high (90%) as compared to market vegetables of Taj Bagh, Railway workshop Mandy and Singpura Lahore Mandy was 87%, 86.5% and 87.5% respectively. Regarding nitrogen, nitrate, nitrite, phosphate, sodium and potassium was high in market vegetables as compared to house samples due to the accumulation of nutrients in soil and crop. (author)

Ecogeomorphology of Sand Dunes Shaped by Vegetation

Science.gov (United States)

Tsoar, H.

2014-12-01

Two dune types associated with vegetation are known: Parabolic and Vegetated Linear Dunes (VLDs), the latters are the dominant dune type in the world deserts. Parabolic dunes are formed in humid, sub-humid and semi-arid environments (rather than arid) where vegetation is nearby. VLDs are known today in semiarid and arid lands where the average yearly rainfall is ≥100 mm, enough to support sparse cover of vegetation. These two dune types are formed by unidirectional winds although they demonstrate a different form and have a distinct dynamics. Conceptual and mathematical models of dunes mobility and stability, based on three control parameters: wind power (DP), average annual precipitation (p), and the human impact parameter (μ) show that where human impact is negligible the effect of wind power (DP) on vegetative cover is substantial. The average yearly rainfall of 60-80 mm is the threshold of annual average rainfall for vegetation growth on dune sand. The model is shown to follow a hysteresis path, which explains the bistability of active and stabilized dunes under the same climatic conditions with respect to wind power. We have discerned formation of parabolic dunes from barchans and transverse dunes in the coastal plain of Israel where a decrease in human activity during the second half of the 20th century caused establishment of vegetation on the crest of the dunes, a process that changed the dynamics of these barchans and transverse dunes and led to a change in the shape of the windward slope from convex to concave. These dunes gradually became parabolic. It seems that VLDs in Australia or the Kalahari have always been vegetated to some degree, though the shrubs were sparser in colder periods when the aeolian erosion was sizeable. Those ancient conditions are characterized by higher wind power and lower rainfall that can reduce, but not completely destroy, the vegetation cover, leading to the formation of lee (shadow) dunes behind each shrub. Formation of

Modelling post-fire vegetation recovery in Portugal

Directory of Open Access Journals (Sweden)

A. Bastos

2011-12-01

Full Text Available Wildfires in Mediterranean Europe have been increasing in number and extension over the last decades and constitute one of the major disturbances of these ecosystems. Portugal is the country with more burnt area in the last decade and the years of 2003 and 2005 were particularly devastating, the total burned areas of 425 000 and 338 000 ha being several times higher than the corresponding average. The year of 2005 further coincided with one of the most severe droughts since early 20th century. Due to different responses of vegetation to diverse fire regimes and to the complexity of landscape structures, fires have complex effects on vegetation recovery. Remote sensing has revealed to be a powerful tool in studying vegetation dynamics and in monitoring post-fire vegetation recovery, which is crucial to land-management and to prevent erosion.

The main goals of the present work are (i to assess the accuracy of a vegetation recovery model previously developed by the authors; (ii to assess the model's performance, namely its sensitivity to initial conditions, to the temporal length of the input dataset and to missing data; (iii to study vegetation recovery over two selected areas that were affected by two large wildfire events in the fire seasons of 2003 and 2005, respectively.

The study relies on monthly values of NDVI over 11 years (1998–2009, at 1 km × 1 km spatial resolution, as obtained by the VEGETATION instrument. According to results from sensitivity analysis, the model is robust and able to provide good estimations of recovery times of vegetation when the regeneration process is regular, even when missing data is present. In respect to the two selected burnt scars, results indicate that fire damage is a determinant factor of regeneration, as less damaged vegetation recovers more rapidly, which is mainly justified by the high coverage of Pinus pinaster over the area, and by the fact that coniferous forests tend to

Semi-Dried Fruits and Vegetables

Directory of Open Access Journals (Sweden)

Gamze Uysal Seçkin

2015-12-01

Full Text Available Since ancient times, the preservation of fruit and vegetables is an ancient method of drying. Sun drying method has been used more widely. In general, consumer-ready products are dried fruits, while the dried vegetables are the foods subjected to the rehydration processes such as boiling, heating and baking before consumption. In recent years, new products with high eating quality have been attempted to achieve without losing characteristic of raw material. With the improving of food technology, using developed methods (pH reduction with reducing aw, slight heating, preservatives use etc. as protective agent, and using a combination of a low rate as an alternative to traditional food preservation process, products have been obtained without changing original characteristics of food. ‘Semi-dried 'or 'medium moist 'products with little difference between the taste and texture of the product with a damp have gained importance in recent years in terms of consumer preferences. Vegetables or fruits, which have water activity levels between 0.50 and 0.95 and the moisture content of between 26% and 60%, are called 'medium moist fruit or vegetables'. Two different manufacturing process to obtain a semi-dried or intermediate moisture products are applied. First, fully dried fruits and vegetables to be rehydrated with water are brought to the desired level of their moisture content. Second, in the first drying process, when the product moisture content is reduced to the desired level, the drying process is finished. The semi-dried products are preferred by consumers because they have a softer texture in terms of eating quality and like fresh products texture.

Vegetation Change in Blue Oak Woodlands in California

Science.gov (United States)

Barbara A. Holzman; Barbara H. Allen-Diaz

1991-01-01

A preliminary report of a statewide project investigating vegetation change in blue oak (Quercus douglasii) woodlands in California is presented. Vegetation plots taken in the 1930s, as part of a statewide vegetation mapping project, were relocated and surveyed. Species composition, cover and tree stand structure data from the earlier study were...

Terrestrial Water Storage and Vegetation Resilience to Drought

Science.gov (United States)

Meyer, V.; Reager, J. T., II; Konings, A. G.

2017-12-01

The expected increased occurrences of hydrologic extreme events such as droughts in the coming decades motivates studies to better understand and predict the response of vegetation to such extreme conditions. Previous studies have addressed vegetation resilience to drought, defined as its ability to recover from a perturbation (Hirota et al., 2011; Vicente-Serrano et al., 2012), but appear to only focus on precipitation and a couple of vegetation indices, hence lacking a key element: terrestrial water storage (TWS). In this study, we combine and compare multiple remotely-sensed hydro-ecological datasets providing information on climatic and hydrological conditions (Tropical Rainfall Measuring Mission (TRMM), Gravity Recovery and Climate Experiment (GRACE)) and indices characterizing the state of the vegetation (vegetation water content using Vegetation Optical Depth (VOD) from SMAP (Soil Moisture Active and Passive), Gross Primary Production (GPP) from FluxCom and Specific Fluorescence Intensity (SFI, from GOSat)) to assess the ability of vegetation to face and recover from droughts across the globe. Our results suggest that GRACE hydrological data bridge the knowledge gap between precipitation deficit and vegetation response. All products are aggregated at a 0.5º spatial resolution and a monthly temporal resolution to match the GRACE Mascon product. Despite these coarse spatiotemporal resolutions, we find that the relationship between existing remotely-sensed eco-hydrologic data varies spatially, both in terms of strength of relationship and time lag, showing the response time of vegetation characteristics to hydrological changes and highlighting the role of water storage. A special attention is given to the Amazon river basin, where two well documented droughts occurred in 2005 and 2010, and where a more recent drought occurred in 2015/2016. References : Hirota, Marina, et al. "Global resilience of tropical forest and savanna to critical transitions." Science

ROOT VEGETABLES, BREEDING TRENDS, RESULTS

Directory of Open Access Journals (Sweden)

M. I. Fedorova

2017-01-01

Full Text Available The main advantage of root vegetables is their unique specificity and high economic importance. The benefits and medicinal properties of root vegetables being highly demanded by the market requirements to the commodity are highlighted in the article. The main directions of breeding program for root vegetable crops, including species of Apiaceae family with carrot, parsnips; Chenopodioideae family with red beet; Brassicaceae family with radish, Daikon, Raphanus sativus L. var. lobo Sazonova & Stank, turnip and rutabaga. Initial breeding accessions of carrot, red beet, radish, Daikon, Raphanus sativus L. var. lobo Sazonova & Stank, turnip and rutabaga have been selected out to be used for breeding program for heterosis. The mf and ms breeding lines were developed, and with the use of them the new gene pool was created. Variety supporting breeding program and methods were also proposed. 

Temporal reflectance changes in vegetables

DEFF Research Database (Denmark)

Dissing, Bjørn Skovlund; Clemmensen, Line Katrine Harder; Ersbøll, Bjarne Kjær

2009-01-01

Quality control in the food industry is often performed by measuring various chemical compounds of the food involved. We propose an imaging concept for acquiring high quality multispectral images to evaluate changes of carrots and celeriac over a period of 14 days. Properties originating...... in the surface chemistry of vegetables may be captured in an integrating sphere illumination which enables the creation of detailed surface chemistry maps with a good combination of spectral and spatial resolutions. Prior to multispectral image recording, the vegetables were prefried and frozen at -30Â......°C for four months. During the 14 days of image recording, the vegetables were kept at +5°C in refrigeration. In this period, surface changes and thereby reflectance properties were very subtle. To describe this small variation we employed advanced statistical techniques to search a large featurespace...

NEW GREENHOUSE TECHNOLOGIES FOR VEGETABLE PRODUCTION

Directory of Open Access Journals (Sweden)

S. M. Sirota

2016-01-01

Full Text Available First decade of XXI century is characterized by significant augmentation in vegetable world’s production. Average annual vegetable production has been 346 million tons, and it has exceeded the average annual potato production (318 million tons. It has occurred due to the use of up-to-date technologies for vegetable production and, particularly, in greenhouses. In Russian Federation, the total production of vegetables was 5 275.6 thousand tons in 2015 that was 13.3% more than in 2014. But the total vegetable production in greenhouses was only 722.8 thousand tons, that was 0.7% less than in 2014 (728.1 thousand tons. It can be explained that the old technologies have been used for many greenhouses around Russia. Up-to-date technologies for greenhouses are described in the article. Small-volume hydroponics. Plants are grown in mineral wadding, packed up in the special chutes. Mineral nutrition and water are supplied through special pipe with many branch pipes toward each plant. Advantage: pH and nutrition are maintained, consumption of water and mineral nutrition are optimized, and that improves plants grow control. Expenditures of labor decreased, quality of fruit became better and the yield increased significantly by 45-50 kg/m2 comparing with growing on the soil (25-30 kg/m2. Hydroponics with flowing water (salad production lines. Conveyor for salad and vegetable growing on horizontal moving chutes with flowing water and nutrition was developed. Advantage: high level of automation and mechanization of all processes of growing increased the effectiveness of the use of greenhouse areas (we can place 30% plants more at the same area. Seedling production lines. Production lines for seedlings enable to grow vegetables and leafy vegetables on stationary benches, being furnished with periodical nutrition and water supply at times. Advantage: 700 seedlings additionally on each m2 a year. Future technologies are

Coastwide Reference Monitoring System (CRMS) Vegetation Volume Index: An assessment tool for marsh habitat focused on the three-dimensional structure at CRMS vegetation monitoring stations

Science.gov (United States)

Wood, William B.; Visser, Jenneke M.; Piazza, Sarai C.; Sharp, Leigh A.; Hundy, Laura C.; McGinnis, Tommy E.

2015-12-04

A Vegetation Volume (VV) variable and Vegetation Volume Index (VVI) have been developed for the Coastwide Reference Monitoring System (CRMS). The VV is a measure of the amount of three-dimensional vegetative structure present at each CRMS site and is based on vegetation data collected annually. The VV uses 10 stations per CRMS site to quantify four vegetation layers: carpet, herbaceous, shrub, and tree. For each layer an overall live vegetation percent cover and height are collected to create a layer volume; the individual layer volumes are then summed to generate a site vegetation volume profile. The VV uses the two-dimensional area of live vegetative cover (in square meters) multiplied by the height (in meters) of each layer to produce a volume (in cubic meters) for each layer present in a 2-meter by 2-meter station. These layers are additive, yielding a total volume for each of the 10 herbaceous vegetation stations and an overall CRMS marsh site average.

A qualitative and quantitative analysis of vegetable pricing in supermarket

Science.gov (United States)

Miranda, Suci

2017-06-01

The purpose of this study is to analyze the variables affecting the determination of the sale price of vegetable which is constant over time in a supermarket qualitatively and quantitavely. It focuses on the non-organic vegetable with a fixed selling price over time such as spinach, beet, and parsley. In qualitative analysis, the sale price determination is influenced by the vegetable characteristics: (1) vegetable segmentation (low to high daily consumed); (2) vegetable age (how long it can last related to freshness); which both characteristic relates to the inventory management and ultimately to the sale price in supermarket. While quantitatively, the vegetables are divided into two categories: the leaf vegetable group that the leaves are eaten as a vegetable with the aging product (a) = 0 and the shelf life (t) = 0, and the non-leafy vegetable group with the aging group (a) = a+1 and the shelf life (t) = t+1. The vegetable age (a) = 0 means they only last for one day when they are ordered then they have to terminate. Whereas a+1 is that they have a longer life for more than a day such as beet, white radish, and string beans. The shelf life refers to how long it will be placed in a shelf in supermarket in line with the vegetable age. According to the cost plus pricing method using full price costing approach, production costs, non-production costs, and markup are adjusted differently for each category. There is a holding cost added to the sale price of the non-leafy vegetable, yet it is assumed a 0 holding cost for the leafy vegetable category. The amount of expected margin of each category is correlated to the vegetable characteristics.

New options for conversion of vegetable oils to alternative fuels

Energy Technology Data Exchange (ETDEWEB)

Demirbas, A.; Kara, H. [Selcuk University, Konya (Turkey). Department of Chemical Engineering

2006-05-15

Biodiesel from transesterification of vegetable oils is an excellent alternative fuel. There is, however, a need to develop a direct process for conversion of vegetable oils into gasoline-competitive biodiesel and other petroleum products. Methyl esters of vegetable oils have several outstanding advantages among other new-renewable and clean engine fuel alternatives. The purpose of the transesterification process is to lower the viscosity of vegetable oil. Compared to No. 2 diesel fuel, all of the vegetable oils are much more viscous, whereas methyl esters of vegetable oils are slightly more viscous. The methyl esters are more volatile than those of the vegetable oils. Conversion of vegetable oils to useful fuels involves the pyrolysis and catalytic cracking of the oils into lower molecular products. Pyrolysis produces more biogasoline than biodiesel fuel. Soap pyrolysis products of vegetable oils can be used as alternative diesel engine fuel. The soaps obtained from the vegetable oils can be pyrolyzed into hydrocarbon-rich products. Zinc chloride catalyst contributed greatly to high amounts of hydrocarbons in the liquid product. The yield of ZnCl2 catalytic conversion of the soybean oil reached the maximum 79.9% at 660 K. (author)

Does urban vegetation mitigate air pollution in northern conditions?

International Nuclear Information System (INIS)

Setälä, Heikki; Viippola, Viljami; Rantalainen, Anna-Lea; Pennanen, Arto; Yli-Pelkonen, Vesa

2013-01-01

It is generally accepted that urban vegetation improves air quality and thereby enhances the well-being of citizens. However, empirical evidence on the potential of urban trees to mitigate air pollution is meager, particularly in northern climates with a short growing season. We studied the ability of urban park/forest vegetation to remove air pollutants (NO 2 , anthropogenic VOCs and particle deposition) using passive samplers in two Finnish cities. Concentrations of each pollutant in August (summer; leaf-period) and March (winter, leaf-free period) were slightly but often insignificantly lower under tree canopies than in adjacent open areas, suggesting that the role of foliage in removing air pollutants is insignificant. Furthermore, vegetation-related environmental variables (canopy closure, number and size of trees, density of understorey vegetation) did not explain the variation in pollution concentrations. Our results suggest that the ability of urban vegetation to remove air pollutants is minor in northern climates. -- Highlights: ► The ability of northern urban vegetation to remove air pollutants is minor. ► Vegetation-related environmental variables had no effect on air pollution levels. ► The ability of vegetation to clean air did not differ between summer and winter. ► Dry deposition passive samplers proved applicable in urban air pollution study. -- The ability of urban vegetation to remove air pollutants seems to be minor in northern climates

Diesel fuel from vegetable oil via transesterification and soap pyrolysis

Energy Technology Data Exchange (ETDEWEB)

Demirbas, A.

2002-09-15

Transesterifications of 6 vegetable oil samples in supercritical methanol (SC MeOH) were studied without using any catalyst. Methyl esters of vegetable oils have several outstanding advantages among other new-renewable and clean engine fuel alternatives. The variables affecting the methyl ester yielded during the transesterification reaction, such as the molar ratio of alcohol to vegetable oil and reaction temperature, were investigated. Compared to No. 2 diesel fuel, all of the vegetable oils are much more viscous, while methyl esters of vegetable oils are the slightly more viscous. The methyl esters are more volatile than those of the vegetable oils. The soaps obtained from the vegetable oils can be pyrolyzed into hydrocarbon-rich products. (author)

Predicting children's fussiness with vegetables:the role of feeding practices

OpenAIRE

Holley, Clare E.; Haycraft, Emma; Farrow, Claire

2017-01-01

Vegetables are commonly rejected by children, making it is important to consider factors that are associated with children's fussiness with vegetables. The current study aimed to investigate whether fussiness with vegetables is associated with a number of factors including caregiver and child vegetable consumption; caregivers' general feeding practices; and caregivers' vegetable-specific feeding practices. Caregivers (N = 297) of preschool children completed questionnaire measures of their ch...

Dynamics of climatic characteristics influencing vegetation in Siberia

International Nuclear Information System (INIS)

Shulgina, Tamara M; Genina, Elena Yu; Gordov, Evgeny P

2011-01-01

The spatiotemporal pattern of the dynamics of surface air temperature and precipitation and those bioclimatic indices that are based upon factors which control vegetation cover are investigated. Surface air temperature and precipitation data are retrieved from the ECMWF ERA Interim reanalysis and APHRODITE JMA datasets, respectively, which were found to be the closest to the observational data. We created an archive of bioclimatic indices for further detailed studies of interrelations between local climate and vegetation cover changes, which include carbon uptake changes related to changes of vegetation types and amount, as well as with spatial shifts of vegetation zones. Meanwhile, analysis reveals significant positive trends of the growing season length accompanied by a statistically significant increase of the sums of the growing degree days and precipitation over the south of West Siberia. The trends hint at a tendency for an increase of vegetation ecosystems' productivity across the south of West Siberia (55°–60°N, 59°–84°E) in the past several decades and (if sustained) may lead to a future increase of vegetation productivity in this region.

Purification of Anti-Verticilfium dahliae Protein from Antagonistic Bacterium BDT-25%棉花黄萎菌拮抗细菌BDT-25抑菌蛋白的分离纯化

Institute of Scientific and Technical Information of China (English)

孙瑶; 李宝庆; 马平

2011-01-01

拮抗细菌BDT-25是从根际土壤中分离得到的1株芽孢杆菌，其菌株发酵液产生的抑菌物质对棉花黄萎菌具有较强的抑制作用。为了明确其抑菌活性组分，本研究通过硫酸铵沉淀的方法从BDT-25菌株去菌体发酵液中提取得到对棉花黄萎病菌具有抑制作用的抑菌蛋白，分别采用阴离子交换层析和凝胶过滤层析的方法对其进行了纯化，抑菌活性检测结果显示，P1具有抑菌活性，为有效抑菌活性组分。经SDS—PAGE电泳纯化为单一蛋白带，分子量约为30kDa。%Bacillus subtilis BDT-25 was collected from the soil of cotton land. Its fermentation broth could produce antifungal substances, with the effect of inhibiting the activity of V. dahliae Kleb. In order to determine the antifungal compound, saturation ammonium sulfate method was used to ectract antifungal protein. The protein was purified by anion exchange chromatography and gel filtration chromatography. The results showed that protein P1 had strong antifungal activity. One single band was collected through SDS-PAGE and the molecular weight of P1 was about 30 kDa.

Simulations of Vegetation Impacts on Arctic Climate

Science.gov (United States)

Bonfils, C.; Phillips, T. J.; Riley, W. J.; Post, W. M.; Torn, M. S.

2009-12-01

Because global warming disproportionately influences high-latitude climate, changes in arctic vegetation are in progress. These land-cover changes include redistribution of local vegetation types as well as northward migration of lower-latitude species in response to the increasing warming. The resulting displacement of low-lying tundra vegetation by shrubs and trees darkens the surface, thus accelerating regional warming. As participants in the U.S. Department of Energy IMPACTS Project, we are investigating the potential for abrupt arctic climatic change resulting from such variations in vegetation, among other mechanisms. To estimate the relative magnitudes of effects to be expected from changes in high-latitude land cover, we are conducting several numerical experiments with the Community Climate System Model (CCSM). These experiments include: 1) A “present-day-climate” control experiment with current atmospheric greenhouse-gas concentrations and climatological monthly sea surface temperatures and sea ice extents prescribed, and with “standard” CLM plant functional types (PFTs) specified; 2) A “changed-vegetation-type” experiment that is the same as 1), except that the “standard” PFTs are augmented by additional vegetation types (forbs, sedges, shrubs, mosses, and lichens) that are not presently represented in CLM. This experiment will require information on the location, fractional cover, and physiological parameterizations of these new PFTs. 3) A “changed-vegetation-extent experiment” that is the same as 2), except that the spatial extents of selected PFTs (e.g. shrubs or boreal forest PFTs) are shifted northward from their present locations in the CLM. We will report on the atmospheric climate and land-surface feedbacks associated with these vegetation changes, with emphasis on local and regional surface energy and moisture fluxes and near-surface temperature, humidity, and clouds. Acknowledgments This work was performed under the auspices

Identification and characterization of microRNAs in oilseed rape (Brassica napus) responsive to infection with the pathogenic fungus Verticillium longisporum using Brassica AA (Brassica rapa) and CC (Brassica oleracea) as reference genomes.

Science.gov (United States)

Shen, Dan; Suhrkamp, Ina; Wang, Yu; Liu, Shenyi; Menkhaus, Jan; Verreet, Joseph-Alexander; Fan, Longjiang; Cai, Daguang

2014-11-01

Verticillium longisporum, a soil-borne pathogenic fungus, causes vascular disease in oilseed rape (Brassica napus). We proposed that plant microRNAs (miRNAs) are involved in the plant-V. longisporum interaction. To identify oilseed rape miRNAs, we deep-sequenced two small RNA libraries made from V. longisporum infected/noninfected roots and employed Brassica rapa and Brassica oleracea genomes as references for miRNA prediction and characterization. We identified 893 B. napus miRNAs representing 360 conserved and 533 novel miRNAs, and mapped 429 and 464 miRNAs to the AA and CC genomes, respectively. Microsynteny analysis with the conserved miRNAs and their flanking protein coding sequences revealed 137 AA-CC genome syntenic miRNA pairs and 61 AA and 42 CC genome-unique miRNAs. Sixty-two miRNAs were responsive to the V. longisporum infection. We present data for specific interactions and simultaneously reciprocal changes in the expression levels of the miRNAs and their targets in the infected roots. We demonstrate that miRNAs are involved in the plant-fungus interaction and that miRNA168-Argonaute 1 (AGO1) expression modulation might act as a key regulatory module in a compatible plant-V. longisporum interaction. Our results suggest that V. longisporum may have evolved a virulence mechanism by interference with plant miRNAs to reprogram plant gene expression and achieve infection. © 2014 The Authors. New Phytologist © 2014 New Phytologist Trust.

Biota - 2009 Vegetation Inventory - Lake Ashtabula, ND

Data.gov (United States)

Army Corps of Engineers, Department of the Army, Department of Defense — 2009 Vegetation Classification for Lake Ashtabula, ND Vegetation Project Report, OMBIL Environmental Stewardship - Level 1 Inventory, U.S. Army Corps of Engineers...

Biota - 2011 Vegetation Inventory - Marsh Lake, MN

Data.gov (United States)

Army Corps of Engineers, Department of the Army, Department of Defense — 2011 Vegetation Classification for Marsh Lake, MN Vegetation Project Report, OMBIL Environmental Stewardship - Level 1 Inventory. Marsh Lake is located on the...

Marketing African Leafy Vegetables: Challenges and Opportunities ...

African Journals Online (AJOL)

Marketing African Leafy Vegetables: Challenges and Opportunities in the Kenyan Context. ... The market share of ALVs vis-à-vis other vegetable species, particularly kales, cabbages and ... EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT

Hydraulic and Vegetative Models of Historic Environmental Conditions Isolate the Role of Riparian Vegetation in Inducing Channel Change

Science.gov (United States)

Manners, R.; Schmidt, J. C.; Wheaton, J. M.

2011-12-01

An enduring question in geomorphology is the role of riparian vegetation in inducing or exacerbating channel narrowing. It is typically difficult to isolate the role of vegetation in causing channel narrowing, because narrowing typically occurs where there are changes in stream flow, sediment supply, the invasion of non-native vegetation, and sometimes climate change. Therefore, linkages between changes in vegetation communities and changes in channel form are often difficult to identify. We took a mechanistic approach to isolate the role of the invasive riparian shrub tamarisk (Tamarix spp) in influencing channel narrowing in the Colorado River basin. Detailed geomorphic reconstructions of two sites on the Yampa and Green Rivers, respectively, in Dinosaur National Monument show that channel narrowing has been progressive and that tamarisk encroachment has also occurred; at the same time, dams have been constructed, diversions increased, and spring snowmelt runoff has been occurring earlier in spring. We simulated hydraulic and sediment transport conditions during the two largest floods of record -- 1984 and 2011. Two-dimensional hydraulic models were built to reflect these conditions and allowed us to perform sensitivity tests to determine the dominant determinants of the observed patterns of erosion and deposition. Channel and floodplain topography were constrained through detailed stratigraphic analysis, including precise dating of deposits based on dating of buried tamarisk plants in a series of floodplain trenches and pits. We also used historical air photos to establish past channel topography. To parameterize the influence of riparian vegetation, we developed a model that links detailed terrestrial laser scan (TLS) measurements of stand structure and its corresponding hydraulic roughness at the patch scale to reach-scale riparian vegetation patterns determined from airborne LiDaR (ALS). This model, in conjunction with maps of the ages and establishment

How Much Do Americans Pay for Fruits and Vegetables?

OpenAIRE

Reed, Jane; Frazao, Elizabeth; Itskowitz, Rachel

2004-01-01

Many Americans do not consume the recommended amount of fruits and vegetables. Almost half of Americans think eating more fruits and vegetables would make their diets healthier, so why don't they? One argument is that fruits and vegetables are expensive, especially when purchased fresh. According to an ERS study, a consumer can meet the recommendation of three servings of fruits and four servings of vegetables daily for 64 cents.

Self-crafting vegetable snacks: testing the IKEA-effect in children.

Science.gov (United States)

Raghoebar, Sanne; van Kleef, Ellen; de Vet, Emely

2017-01-01

The purpose of this paper is to test whether the IKEA-effect (Norton et al. , 2012) - better liking for self-crafted products than for identical products crafted by others - can be exploited to increase liking and consumption of vegetable snacks in children. A between-subjects experiment was conducted at an after school care facility. In total, 86 children aged four to six either crafted a peacock with vegetables or with non-food objects following an example. After the task, children ate snack vegetables ad libitum, and rated their liking for the vegetables and pride in crafting the peacock. No significant main effect of the vegetable snack creation on consumption and liking was observed. Also, perceived pride did not mediate the effect of self-crafting vegetable snacks on consumption of and liking for vegetables. Vegetable consumption did not differ between children who were either simply exposed to vegetable snacks while crafting or those who were crafting the vegetable snacks themselves. The equal consumption might suggest that this is caused by simple exposure, but more research is needed comparing self-crafting and exposure to a condition where there is no initial exposure to vegetables. Although the IKEA-effect has been demonstrated in adults, this is one of the first studies evaluating the IKEA-effect in children and as a means to increase liking for a generally disliked product in this target group, i.e. vegetables. The IKEA-effect could not be replicated under these more stringent conditions, where the experimental set-up enabled disentangling exposure and crafting effects.

Intake of Raw Fruits and Vegetables Is Associated With Better Mental Health Than Intake of Processed Fruits and Vegetables

Science.gov (United States)

Brookie, Kate L.; Best, Georgia I.; Conner, Tamlin S.

2018-01-01

Background: Higher intakes of fruits and vegetables, rich in micronutrients, have been associated with better mental health. However, cooking or processing may reduce the availability of these important micronutrients. This study investigated the differential associations between intake of raw fruits and vegetables, compared to processed (cooked or canned) fruits and vegetables, and mental health in young adults. Methods: In a cross-sectional survey design, 422 young adults ages 18–25 (66.1% female) living in New Zealand and the United States completed an online survey that assessed typical consumption of raw vs. cooked/canned/processed fruits and vegetables, negative and positive mental health (depressive symptoms, anxiety, negative mood, positive mood, life satisfaction, and flourishing), and covariates (including socio-economic status, body mass index, sleep, physical activity, smoking, and alcohol use). Results: Controlling for covariates, raw fruit and vegetable intake (FVI) predicted reduced depressive symptoms and higher positive mood, life satisfaction, and flourishing; processed FVI only predicted higher positive mood. The top 10 raw foods related to better mental health were carrots, bananas, apples, dark leafy greens like spinach, grapefruit, lettuce, citrus fruits, fresh berries, cucumber, and kiwifruit. Conclusions: Raw FVI, but not processed FVI, significantly predicted higher mental health outcomes when controlling for the covariates. Applications include recommending the consumption of raw fruits and vegetables to maximize mental health benefits. PMID:29692750

Intake of Raw Fruits and Vegetables Is Associated With Better Mental Health Than Intake of Processed Fruits and Vegetables

Directory of Open Access Journals (Sweden)

Kate L. Brookie

2018-04-01

Full Text Available Background: Higher intakes of fruits and vegetables, rich in micronutrients, have been associated with better mental health. However, cooking or processing may reduce the availability of these important micronutrients. This study investigated the differential associations between intake of raw fruits and vegetables, compared to processed (cooked or canned fruits and vegetables, and mental health in young adults.Methods: In a cross-sectional survey design, 422 young adults ages 18–25 (66.1% female living in New Zealand and the United States completed an online survey that assessed typical consumption of raw vs. cooked/canned/processed fruits and vegetables, negative and positive mental health (depressive symptoms, anxiety, negative mood, positive mood, life satisfaction, and flourishing, and covariates (including socio-economic status, body mass index, sleep, physical activity, smoking, and alcohol use.Results: Controlling for covariates, raw fruit and vegetable intake (FVI predicted reduced depressive symptoms and higher positive mood, life satisfaction, and flourishing; processed FVI only predicted higher positive mood. The top 10 raw foods related to better mental health were carrots, bananas, apples, dark leafy greens like spinach, grapefruit, lettuce, citrus fruits, fresh berries, cucumber, and kiwifruit.Conclusions: Raw FVI, but not processed FVI, significantly predicted higher mental health outcomes when controlling for the covariates. Applications include recommending the consumption of raw fruits and vegetables to maximize mental health benefits.

Is repeated exposure the holy grail for increasing children's vegetable intake? Lessons learned from a Dutch childcare intervention using various vegetable preparations

NARCIS (Netherlands)

Zeinstra, Gertrude G.; Vrijhof, Milou; Kremer, Stefanie

2018-01-01

Children's failure to eat enough vegetables highlights the need for effective interventions encouraging this behaviour. The aim of this study was to investigate the effect of repeated exposure to three a priori unfamiliar vegetables, each prepared in two ways, on children's vegetable acceptance in a

Biological effects of fruit and vegetables.

Science.gov (United States)

Dragsted, Lars O; Krath, Britta; Ravn-Haren, Gitte; Vogel, Ulla B; Vinggaard, Anne Marie; Bo Jensen, Per; Loft, Steffen; Rasmussen, Salka E; Sandstrom, The late BrittMarie; Pedersen, Anette

2006-02-01

A strong and persistent effect of plant-derived foods on the prevention of lifestyle diseases has emerged from observational studies. Several groups of constituents in plants have been identified as potentially health promoting in animal studies, including cholesterol-lowering factors, antioxidants, enzyme inducers, apoptosis inducers etc. In human intervention studies the dose levels achieved tend to be lower than the levels found to be effective in animals and sampling from target organs is often not possible. A controlled dietary human intervention study was performed with forty-three volunteers, providing 600 g fruit and vegetables/d or in the controls a carbohydrate-rich drink to balance energy intake. Surrogate markers of oxidative damage to DNA, protein and lipids, enzymic defence and lipid metabolism were determined in blood and urine. It was found that a high intake of fruit and vegetables tends to increase the stability of lipids towards oxidative damage. Markers of oxidative enzymes indicate a steady increase in glutathione peroxidase (GPX1) activity in erythrocytes during intervention with fruit and vegetables but there is no effect on GPX1 transcription levels in leucocytes. No change occurs in glutathione-conjugating or -reducing enzyme activities in erythrocytes or plasma, and there are no effects on the transcription of genes involved in phase 2 enzyme induction or DNA repair in leucocytes. Fruit and vegetable intake decreases the level of total cholesterol and LDL-cholesterol, but does not affect sex hormones. In conclusion, it has been shown that total cholesterol and LDL-cholesterol, markers of peripheral lipid oxidation, and erythrocyte GPX1 activity are affected by high intakes of fruit and vegetables. This finding provides support for a protective role of dietary fruit and vegetables against CVD.

Sensitivity Analysis of a Riparian Vegetation Growth Model

Directory of Open Access Journals (Sweden)

Michael Nones

2016-11-01

Full Text Available The paper presents a sensitivity analysis of two main parameters used in a mathematic model able to evaluate the effects of changing hydrology on the growth of riparian vegetation along rivers and its effects on the cross-section width. Due to a lack of data in existing literature, in a past study the schematization proposed here was applied only to two large rivers, assuming steady conditions for the vegetational carrying capacity and coupling the vegetal model with a 1D description of the river morphology. In this paper, the limitation set by steady conditions is overcome, imposing the vegetational evolution dependent upon the initial plant population and the growth rate, which represents the potential growth of the overall vegetation along the watercourse. The sensitivity analysis shows that, regardless of the initial population density, the growth rate can be considered the main parameter defining the development of riparian vegetation, but it results site-specific effects, with significant differences for large and small rivers. Despite the numerous simplifications adopted and the small database analyzed, the comparison between measured and computed river widths shows a quite good capability of the model in representing the typical interactions between riparian vegetation and water flow occurring along watercourses. After a thorough calibration, the relatively simple structure of the code permits further developments and applications to a wide range of alluvial rivers.

Biofilm formation enhances Helicobacter pylori survivability in vegetables.

Science.gov (United States)

Ng, Chow Goon; Loke, Mun Fai; Goh, Khean Lee; Vadivelu, Jamuna; Ho, Bow

2017-04-01

To date, the exact route and mode of transmission of Helicobacter pylori remains elusive. The detection of H. pylori in food using molecular approaches has led us to postulate that the gastric pathogen may survive in the extragastric environment for an extended period. In this study, we show that H. pylori prolongs its survival by forming biofilm and micro-colonies on vegetables. The biofilm forming capability of H. pylori is both strain and vegetable dependent. H. pylori strains were classified into high and low biofilm formers based on their highest relative biofilm units (BU). High biofilm formers survived longer on vegetables compared to low biofilm formers. The bacteria survived better on cabbage compared to other vegetables tested. In addition, images captured on scanning electron and confocal laser scanning microscopes revealed that the bacteria were able to form biofilm and reside as micro-colonies on vegetable surfaces, strengthening the notion of possible survival of H. pylori on vegetables for an extended period of time. Taken together, the ability of H. pylori to form biofilm on vegetables (a common food source for human) potentially plays an important role in its survival, serving as a mode of transmission of H. pylori in the extragastric environment. Copyright © 2016 Elsevier Ltd. All rights reserved.

Understory vegetation

Science.gov (United States)

Steve Sutherland; Todd F. Hutchinson; Jennifer L. Windus

2003-01-01

This chapter documents patterns of species composition and diversity within the understory vegetation layer and provides a species list for the four study areas in southern Ohio. Within each of 108 plots, we recorded the frequency of all vascular plant species in sixteen 2-m² quadrats. We recorded 297 species, including 187 forbs (176 perennials, 9 annuals, 2...

Advances on Modelling Riparian Vegetation-Hydromorphology Interactions

NARCIS (Netherlands)

Solari, L.; Van Oorschot, M.; Belletti, B.; Hendriks, D.; Rinaldi, M.; Vargas-Luna, A.

2016-01-01

Riparian vegetation actively interacts with fluvial systems affecting river hydrodynamics, morphodynamics and groundwater. These interactions can be coupled because both vegetation and hydromorphology (i.e. the combined scientific study of hydrology and fluvial geomorphology) involve dynamic

[Simulation of vegetation indices optimizing under retrieval of vegetation biochemical parameters based on PROSPECT + SAIL model].

Science.gov (United States)

Wu, Ling; Liu, Xiang-Nan; Zhou, Bo-Tian; Liu, Chuan-Hao; Li, Lu-Feng

2012-12-01

This study analyzed the sensitivities of three vegetation biochemical parameters [chlorophyll content (Cab), leaf water content (Cw), and leaf area index (LAI)] to the changes of canopy reflectance, with the effects of each parameter on the wavelength regions of canopy reflectance considered, and selected three vegetation indices as the optimization comparison targets of cost function. Then, the Cab, Cw, and LAI were estimated, based on the particle swarm optimization algorithm and PROSPECT + SAIL model. The results showed that retrieval efficiency with vegetation indices as the optimization comparison targets of cost function was better than that with all spectral reflectance. The correlation coefficients (R2) between the measured and estimated values of Cab, Cw, and LAI were 90.8%, 95.7%, and 99.7%, and the root mean square errors of Cab, Cw, and LAI were 4.73 microg x cm(-2), 0.001 g x cm(-2), and 0.08, respectively. It was suggested that to adopt vegetation indices as the optimization comparison targets of cost function could effectively improve the efficiency and precision of the retrieval of biochemical parameters based on PROSPECT + SAIL model.

Intensity of competition in the market of greenhouse vegetables

Directory of Open Access Journals (Sweden)

Oleg Ivanovich Botkin

2012-03-01

Full Text Available This paper reviews the competitive environment of the market greenhouse vegetables. Revealed specific features of the industry, determining the level of intensity of competition in the market greenhouse vegetables. Classified factors internal and external environment, identify indicators that affect the state of the market. The factors that determine the intensity of competition in the market greenhouse vegetables.The main competitors on the Russian market of greenhouse production.Identified indicators of the intensity level of competition, in particular: the level of monopolization of the market greenhouse vegetables, the level of concentration of production in the industry, the generalized index of the intensity of the competitive environment.Shows a comparative analysis of competitors’ market greenhouse vegetables in Udmurtia.Revealed competitive advantages which can help local producers to reduce the pressure of competition and intra-industry to occupy a leading position in the Russian market of greenhouse vegetable production.The dynamics of economic performance of Russian producers. Ways of improving the competitiveness of enterprises for the production of greenhouse vegetables

Local vegetables in Cameroon: Corchorus species used as a vegetable.

NARCIS (Netherlands)

Westphal-Stevels, J.M.C.

1986-01-01

An agro-botanical study of local vegetables in Cameroon is in preparation, including the taxonomy, identity, morphology, agronomy and nutritional value of about 70 species. Corchorus olitorius L. and other edible species of the genus Corchorus L. (Tiliaceae) are part of this study. The wide

Rendering Future Vegetation Change across Large Regions of the US

Science.gov (United States)

Sant'Anna Dias, Felipe; Gu, Yuting; Agarwalla, Yashika; Cheng, Yiwei; Patil, Sopan; Stieglitz, Marc; Turk, Greg

2015-04-01

We use two Machine Learning techniques, Decision Trees (DT) and Neural Networks (NN), to provide classified images and photorealistic renderings of future vegetation cover at three large regions in the US. The training data used to generate current vegetation cover include Landsat surface reflectance images, USGS Land Cover maps, 50 years of mean annual temperature and precipitation for the period 1950 - 2000, elevation, aspect and slope data. Present vegetation cover was generated on a 100m grid. Future vegetation cover for the period 2061- 2080 was predicted using the 1 km resolution bias corrected data from the NASA Goddard Institute for Space Studies Global Climate Model E simulation. The three test regions encompass a wide range of climatic gradients, topographic variation, and vegetation cover. The central Oregon site covers 19,182 square km and includes the Ochoco and Malheur National Forest. Vegetation cover is 50% evergreen forest and 50% shrubs and scrubland. The northwest Washington site covers 14,182 square km. Vegetation cover is 60% evergreen forest, 14% scrubs, 7% grassland, and 7% barren land. The remainder of the area includes deciduous forest, perennial snow cover, and wetlands. The third site, the Jemez mountain region of north central New Mexico, covers 5,500 square km. Vegetation cover is 47% evergreen forest, 31% shrubs, 13% grasses, and 3% deciduous forest. The remainder of the area includes developed and cultivated areas and wetlands. Using the above mentioned data sets we first trained our DT and NN models to reproduce current vegetation. The land cover classified images were compared directly to the USGS land cover data. The photorealistic generated vegetation images were compared directly to the remotely sensed surface reflectance maps. For all three sites, similarity between generated and observed vegetation cover was quite remarkable. The three trained models were then used to explore what the equilibrium vegetation would look like for

Stages of change to increase fruit and vegetable intake and its relationships with fruit and vegetable intake and related psychosocial factors.

Science.gov (United States)

Chee Yen, Wong; Mohd Shariff, Zalilah; Kandiah, Mirnalini; Mohd Taib, Mohd Nasir

2014-06-01

Understanding individual's intention, action and maintenance to increase fruit and vegetable intake is an initial step in designing nutrition or health promotion programs. This study aimed to determine stages of change to increase fruit and vegetable intake and its relationships with fruit and vegetable intake, self-efficacy, perceived benefits and perceived barriers. This cross-sectional study was conducted among 348 public university staff in Universiti Putra Malaysia. A pre-tested self-administered questionnaire and two days 24-hour diet recall were used. Half of the respondents (50%) were in preparation stage, followed by 43% in action/maintenance, 7% in pre-contemplation/contemplation stages. Respondents in action/maintenance stages had significantly higher self-efficacy (F = 9.17, P diet high in fruits and vegetables in order to promote healthy changes in having high fruit and vegetable intake.

Quantitative indicators of fruit and vegetable consumption

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Dagmar Kozelová

2015-12-01

Full Text Available The quantitative research of the market is often based on surveys and questionnaires which are finding out the behavior of customers in observed areas. Before purchasing process consumers consider where they will buy fruit and vegetables, what kind to choose and in what quantity of goods. Consumers' behavior is affected by the factors as: regional gastronomic traditions, price, product appearance, aroma, place of buying, own experience and knowledge, taste preferences as well as specific health issues of consumers and others. The consumption of fruit and vegetables brings into the human body biological active substances that favorably affect the health of consumers. In the presented research study we were interested in differences of consumers' behavior in the consumption of fruit and vegetables according to the place of residence and gender. In the survey 200 respondents has participated; their place of residence was city or village. The existence of dependences and statistical significance were examined by selected statistical testing methods. Firstly we analyzed the responses via statistical F-test whether observed random samples have the same variance. Then we applied two-sample unpaired t-test with equal variance and χ2-test of statistical independence. The statistical significance was tested by corresponding p values. Correlations were proved by the Cramer's V coefficient. We found that place of residence has no impact on the respondents' consumption of fruit. The gender of respondents does not affect their consumption of fruit. Equally, the gender does not affect the respondents' consumption of vegetables. Only in one observed case the significant differences proved that the place of respondent residence has impact on the consumption of vegetables. Higher consumption of vegetables is due to the fact that the majority of citizens, who live in villages, have a possibility to grow their own vegetables and, thus, the demand for it in village

Study on the air quality near vegetation along the highway

International Nuclear Information System (INIS)

Weijers, E.P.; Kos, G.P.A.; Van den Bulk, W.C.M.; Vermeulen, A.T.

2007-08-01

The results of downwind measurements of air quality around vegetation near a motorway are presented and discussed. The study (first ever in the Netherlands) was performed by order of the Innovation Programme Air Quality. At a carefully selected location measurements of PM and NOx were carried out downwind from a motorway in front of and (at various distances) behind a parallel orientated vegetation strip. Results are compared with synchronized data collected at a nearby location without vegetation (the 'reference' situation) and at the same distances from the motorway. Major findings are: Comparing measurements in front of and behind the vegetation strip show that the presence of vegetation tends to lower PM10 and PM2.5 concentrations immediately behind the vegetation. However, no such change is apparent in the concentrations of NO and NO2. The comparison with measurements executed without vegetation ('reference') indicate that concentration levels close to the vegetation strip are similar or somewhat higher. At a larger distance from motorway and vegetation (>30 m) NO and NO2 concentrations tend to be lower than what is observed in the reference situation. This could not be determined for PM [nl

Levels of lead, cadmium and zinc in vegetables

Energy Technology Data Exchange (ETDEWEB)

Fuchs, G.; Haegglund, J.; Jorhem, L.

1976-01-01

The concentrations of lead, cadmium and zinc have been determined in 455 samples of fresh fruit, vegetables and mushrooms by dry ashing and atomic absorption spectrophotometry. The lead content in all samples was in the range < 0.001-0.288 mg/kg, the mean being 0.02 mg/kg. Leaf vegetables (lettuce and spinach) showed higher values, mean 0.04 mg/kg. The mean values of the cadmium content in fruit, green vegetables, potatoes and root vegetables were 0.003, 0.013, 0.016 and 0.038 mg/kg respectively. The zinc contents were in the ppm range. The ratio Zn/Cd was also determined in some samples. All values concern edible parts and are calculated on wet weight basis. The fruit and vegetables were estimated to constitute about 2 percent and 8 percent respectively of the provisional tolerable weekly intake of these metals recommended by an FAO/WHO Expert Committee.

SEBARAN DAN EFIKASI BERBAGAI GENUS CENDAWAN ENTOMOPATOGEN TERHADAP Riptortus linearis PADA KEDELAI DI LAMPUNG DAN SUMATRA SELATAN

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Yusmani Prayogo .

2012-02-01

Full Text Available Distribution and efficacy on various entomopathogenic fungi at Lampung and South Sumatra as a biological control agent against Riptortus linearis. This study was conducted from June to September 2005.  The fungi were isolated from insect cadavers, insect bait, and soil sample from the soybean land. Each fungal sample was identified  base on their morphology using determination keys.  The fungal isolates were inoculated to the pod sucking bug Riptortus linearis.  The results showed that there were six genera of entomopathogenic fungi that can be isolated, i.e.  Fusarium sp., Penicillium sp., Metarhizium sp., Verticillium sp., Paecilomyces sp., and  Beauveria sp.  The fungus-induced  mortality of R. linearis varied between 5 - 30%.  Paecilomyces sp. isolated from Lebak Batang Baru induced 25%; Beauveria sp. isolated from Pulung Kencana 25%; Verticillium sp. isolated from Kaliungu 20%. Metarhizium sp. isolated from Terbanggi Subing 20% and Verticillium sp. isolated from Lebak batang Baru 20% mortality. It was suggested that these fungi have potential as biological control agents  for the pod sucking bug in dry acid land.

IgE Sensitization to Bacterial and Fungal Biopesticides in a Cohort of Danish Greenhouse Workers

DEFF Research Database (Denmark)

Doekes, G.; Larsen, Preben; Sigsgaard, T.

2004-01-01

BACKGROUND: The use of biopesticides in agriculture may implicate new risks of work-related allergic reactions. METHODS: Sera were tested from the BIOGART project, a longitudinal respiratory health study among >300 Danish greenhouse workers. IgE was measured by enzyme immunoassay (EIA) with extra......BACKGROUND: The use of biopesticides in agriculture may implicate new risks of work-related allergic reactions. METHODS: Sera were tested from the BIOGART project, a longitudinal respiratory health study among >300 Danish greenhouse workers. IgE was measured by enzyme immunoassay (EIA......) with extracts of biopesticide products containing Bacillus thuringiensis (BT) or Verticillium lecanii (Vert). RESULTS: Many sera had detectable IgE to BT (23-29%) or Vert (9-21%). IgE titers from the 2- and 3-year follow-up (n=230) were highly correlated, with discordant results in ... BT, or to different Verticillium products were also significantly correlated (both r >0.70), whereas IgE anti-BT and anti-Verticillium showed no correlation at all. CONCLUSIONS: Exposure to these microbial biopesticides may confer a risk of IgE-mediated sensitization. In future research...

Biologically active matters in soy vegetative mass

OpenAIRE

GNOEVYY I.V.

2013-01-01

Maintenance of row of bioactive matters of phenic nature in particular, isoflavonoids is investigational at to soy-bean cakes to vegetative mass of soy in the phases of vegetation of 8 the most widespread sorts of soy in Ukraine.

Fresh Fruit and Vegetable Program and Requests for Fruits and Vegetables Outside School Settings.

Science.gov (United States)

Ohri-Vachaspati, Punam; Dachenhaus, Elizabeth; Gruner, Jessie; Mollner, Kristina; Hekler, Eric B; Todd, Michael

2018-01-08

Consumption of fruits and vegetables (F/V) among elementary school-aged children remains inadequate, especially among low-income children. The US Department of Agriculture's Fresh Fruit and Vegetable Program (FFVP) provides F/V as snacks to children during the school day, outside of school meals. School-based initiatives are successful in changing behaviors in school settings; however, their influence on behaviors outside of schools needs investigation. To examine whether FFVP participation is associated with F/V requests at stores, self-efficacy to ask for and choose F/V at home, and F/V consumption. Cross-sectional study. Fourth graders in six classrooms (n=296) from three urban, low-income school districts in Phoenix, AZ, were surveyed during 2015; one FFVP and one non-FFVP school from each district that were similar in school size, percent free/reduced-price meal eligibility, and race/ethnicity of enrolled students were selected. Children's self-reported F/V requests during shopping, their self-efficacy to ask for and choose F/V at home, and F/V consumption on the previous day (non-FFVP school day) were measured using questions adapted from validated surveys. Multivariable mixed-effect regression models, adjusting for clustering of students within classes and classes within schools were explored. In models adjusting for individual-level factors (ie, age and sex) only, several significant positive associations were observed between school FFVP participation and healthier F/V outcomes. After additionally adjusting for school-level factors (ie, total enrollment and % Hispanic/Latino students) significant associations were observed between school FFVP participation and more requests for vegetables during shopping (Pvegetables at home (P=0.004), stronger preferences for vegetables (Pfruit (P=0.006). School FFVP participation was associated with more requests for vegetables during shopping and higher self-efficacy to make healthy choices at home, suggesting the

Estimation of vegetation cover resilience from satellite time series

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T. Simoniello

2008-07-01

Full Text Available Resilience is a fundamental concept for understanding vegetation as a dynamic component of the climate system. It expresses the ability of ecosystems to tolerate disturbances and to recover their initial state. Recovery times are basic parameters of the vegetation's response to forcing and, therefore, are essential for describing realistic vegetation within dynamical models. Healthy vegetation tends to rapidly recover from shock and to persist in growth and expansion. On the contrary, climatic and anthropic stress can reduce resilience thus favouring persistent decrease in vegetation activity.

In order to characterize resilience, we analyzed the time series 1982–2003 of 8 km GIMMS AVHRR-NDVI maps of the Italian territory. Persistence probability of negative and positive trends was estimated according to the vegetation cover class, altitude, and climate. Generally, mean recovery times from negative trends were shorter than those estimated for positive trends, as expected for vegetation of healthy status. Some signatures of inefficient resilience were found in high-level mountainous areas and in the Mediterranean sub-tropical ones. This analysis was refined by aggregating pixels according to phenology. This multitemporal clustering synthesized information on vegetation cover, climate, and orography rather well. The consequent persistence estimations confirmed and detailed hints obtained from the previous analyses. Under the same climatic regime, different vegetation resilience levels were found. In particular, within the Mediterranean sub-tropical climate, clustering was able to identify features with different persistence levels in areas that are liable to different levels of anthropic pressure. Moreover, it was capable of enhancing reduced vegetation resilience also in the southern areas under Warm Temperate sub-continental climate. The general consistency of the obtained results showed that, with the help of suited analysis

An ergonomic approach for designing indian traditional vegetable cutter.

Science.gov (United States)

Dhara, Prakash C; De, Sujaya; Sengupta, Piyali; Maity, Payel; Pal, Amitava

2015-01-01

In India varieties of hand tools have been used to cut the vegetables. Traditional vegetable cutter is a commonly used hand tool which has been used for years in the kitchen. The tool may have some design related problems. The present study was undertaken to reduce those problems. The study objective was to evaluate a new design of traditional vegetable cutters for use in the Indian kitchen. One hundred and fifty Indian women who regularly used a vegetable cutter for cooking purposes participated in this study. The design of the vegetable cutter was modified based on the postural preference of the users and other anthropometric factors including the blade angle, length, breadth and width of the sitting area. The prevalence of musculoskeletal disorders was assessed by means of a questionnaire for subjects' feedback. New concepts of the design were proposed and a few prototypes were made and were tested by paired comparison using the EMG system. A large number of subjects (61%) used the vegetable cutter while sitting on the floor with folded knees and the prevalence of MSD in most of the body parts was comparatively lower in this posture than that in squatting posture. In the new design, a broad platform was suggested to provide a more comfortable sitting when a subject sits on it with folded knees. For the vegetable cutter, the blade angle was made at 120° with a broad folded wooden base as the final prototype of the cutter. The length, breadth, and thickness of the base were selected based on the results of the anthropometric measurements among the prototypes of the cutters. The selected vegetable cutter showed the least myoelectric activity among the prototypes during cutting vegetables. The modified vegetable cutter appeared to be ergonomically effective, less prone to muscular stress, and compatible for preferred posture of the users.

Monitoring temporal Vegetation changes in Lao tropical forests

International Nuclear Information System (INIS)

Phompila, Chittana; Lewis, Megan; Clarke, Kenneth; Ostendorf, Bertram

2014-01-01

Studies on changes in vegetation are essential for understanding the interaction between humans and the environment. These studies provide key information for land use assessment, terrestrial ecosystem monitoring, carbon flux modelling and impacts of global climate change. The primary purpose of this study was to detect temporal vegetation changes in tropical forests in the southern part of Lao PDR from 2001-2012. The study investigated the annual vegetation phenological response of dominant land cover types across the study area and relationships to seasonal precipitation and temperature. Improved understanding of intra-annual patterns of vegetation variation was useful to detect longer term changes in vegetation. The breaks for additive season and trend (BFAST) approach was implemented to detect changes in these land cover types throughout the 2001-2012 period. We used the enhanced vegetation index (EVI) data from the Moderate Resolution Imaging Spectroradiometer (MODIS) (MOD13Q1 products) and monthly rainfall and temperature data obtained from the Meteorology and Hydrology Department, Ministry of Agriculture-Forestry, published by Lao National Statistical Centre in this research. EVI well documented the annual seasonal growth of vegetation and clearly distinguished the characteristic phenology of four different land use types; native forest, plantation, agriculture and mixed wooded/cleared area. Native forests maintained high EVI throughout the year, while plantations, wooded/cleared areas and agriculture showed greater inter-annual variation, with minimum EVI at the end of the dry season in April and maximum EVI in September-October, around two months after the wet season peak in rainfall. The BFAST analysis detected abrupt temporal changes in vegetation in the tropical forests, especially in a large conversion of mixed wooded/cleared area into plantation. Within the study area from 2001-2012 there has been an overall decreasing trend of vegetation cover for

Post-fire vegetation dynamics in Portugal

Science.gov (United States)

Gouveia, C.; Dacamara, C. C.; Trigo, R. M.

2009-04-01

The number of fires and the extent of the burned surface in Mediterranean Europe have increased significantly during the last three decades. This may be due either to modifications in land-use (e.g. land abandonment and fuel accumulation) or to climatic changes (e.g. reduction of fuel humidity), both factors leading to an increase of fire risk and fire spread. As in the Mediterranean ecosystems, fires in Portugal have an intricate effect on vegetation regeneration due to the complexity of landscape structures as well as to the different responses of vegetation to the variety of fire regimes. A thorough evaluation of vegetation recovery after fire events becomes therefore crucial in land management. In the above mentioned context remote sensing plays an important role because of its ability to monitor and characterise post-fire vegetation dynamics. A number of fire recovery studies, based on remote sensing, have been conducted in regions characterised by Mediterranean climates and the use of NDVI to monitor plant regeneration after fire events was successfully tested (Díaz-Delgado et al., 1998). In particular, several studies have shown that rapid regeneration occurs within the first 2 years after the fire occurrences, with distinct recovery rates according to the geographical facing of the slopes (Pausas and Vallejo, 1999). In 2003 Portugal was hit by the most devastating sequence of large fires, responsible by a total burnt area of 450 000 ha (including 280 000 ha of forest), representing about 5% of the Portuguese mainland (Trigo et al., 2006). The aim of the present work is to assess and monitor the vegetation behaviour over Portugal following the 2003 fire episodes. For this purpose we have used the regional fields of the Normalized Difference Vegetation Index (NDVI) as obtained from the VEGETATION-SPOT5 instrument, from 1999 to 2008. We developed a methodology to identify large burnt scars in Portugal for the 2003 fire season. The vegetation dynamics was then

Global vegetation change predicted by the modified Budyko model

Energy Technology Data Exchange (ETDEWEB)

Monserud, R.A.; Tchebakova, N.M.; Leemans, R. (US Department of Agriculture, Moscow, ID (United States). Intermountain Research Station, Forest Service)

1993-09-01

A modified Budyko global vegetation model is used to predict changes in global vegetation patterns resulting from climate change (CO[sub 2] doubling). Vegetation patterns are predicted using a model based on a dryness index and potential evaporation determined by solving radiation balance equations. Climate change scenarios are derived from predictions from four General Circulation Models (GCM's) of the atmosphere (GFDL, GISS, OSU, and UKMO). All four GCM scenarios show similar trends in vegetation shifts and in areas that remain stable, although the UKMO scenario predicts greater warming than the others. Climate change maps produced by all four GCM scenarios show good agreement with the current climate vegetation map for the globe as a whole, although over half of the vegetation classes show only poor to fair agreement. The most stable areas are Desert and Ice/Polar Desert. Because most of the predicted warming is concentrated in the Boreal and Temperate zones, vegetation there is predicted to undergo the greatest change. Most vegetation classes in the Subtropics and Tropics are predicted to expand. Any shift in the Tropics favouring either Forest over Savanna, or vice versa, will be determined by the magnitude of the increased precipitation accompanying global warming. Although the model predicts equilibrium conditions to which many plant species cannot adjust (through migration or microevolution) in the 50-100 y needed for CO[sub 2] doubling, it is not clear if projected global warming will result in drastic or benign vegetation change. 72 refs., 3 figs., 3 tabs.

Global changes in dryland vegetation dynamics (1988–2008 assessed by satellite remote sensing: comparing a new passive microwave vegetation density record with reflective greenness data

Directory of Open Access Journals (Sweden)

N. Andela

2013-10-01

Full Text Available Drylands, covering nearly 30% of the global land surface, are characterized by high climate variability and sensitivity to land management. Here, two satellite-observed vegetation products were used to study the long-term (1988–2008 vegetation changes of global drylands: the widely used reflective-based Normalized Difference Vegetation Index (NDVI and the recently developed passive-microwave-based Vegetation Optical Depth (VOD. The NDVI is sensitive to the chlorophyll concentrations in the canopy and the canopy cover fraction, while the VOD is sensitive to vegetation water content of both leafy and woody components. Therefore it can be expected that using both products helps to better characterize vegetation dynamics, particularly over regions with mixed herbaceous and woody vegetation. Linear regression analysis was performed between antecedent precipitation and observed NDVI and VOD independently to distinguish the contribution of climatic and non-climatic drivers in vegetation variations. Where possible, the contributions of fire, grazing, agriculture and CO2 level to vegetation trends were assessed. The results suggest that NDVI is more sensitive to fluctuations in herbaceous vegetation, which primarily uses shallow soil water, whereas VOD is more sensitive to woody vegetation, which additionally can exploit deeper water stores. Globally, evidence is found for woody encroachment over drylands. In the arid drylands, woody encroachment appears to be at the expense of herbaceous vegetation and a global driver is interpreted. Trends in semi-arid drylands vary widely between regions, suggesting that local rather than global drivers caused most of the vegetation response. In savannas, besides precipitation, fire regime plays an important role in shaping trends. Our results demonstrate that NDVI and VOD provide complementary information and allow new insights into dryland vegetation dynamics.

A method for climate and vegetation reconstruction through the inversion of a dynamic vegetation model

Energy Technology Data Exchange (ETDEWEB)

Garreta, Vincent; Guiot, Joel; Hely, Christelle [CEREGE, UMR 6635, CNRS, Universite Aix-Marseille, Europole de l' Arbois, Aix-en-Provence (France); Miller, Paul A.; Sykes, Martin T. [Lund University, Department of Physical Geography and Ecosystems Analysis, Geobiosphere Science Centre, Lund (Sweden); Brewer, Simon [Universite de Liege, Institut d' Astrophysique et de Geophysique, Liege (Belgium); Litt, Thomas [University of Bonn, Paleontological Institute, Bonn (Germany)

2010-08-15

Climate reconstructions from data sensitive to past climates provide estimates of what these climates were like. Comparing these reconstructions with simulations from climate models allows to validate the models used for future climate prediction. It has been shown that for fossil pollen data, gaining estimates by inverting a vegetation model allows inclusion of past changes in carbon dioxide values. As a new generation of dynamic vegetation model is available we have developed an inversion method for one model, LPJ-GUESS. When this novel method is used with high-resolution sediment it allows us to bypass the classic assumptions of (1) climate and pollen independence between samples and (2) equilibrium between the vegetation, represented as pollen, and climate. Our dynamic inversion method is based on a statistical model to describe the links among climate, simulated vegetation and pollen samples. The inversion is realised thanks to a particle filter algorithm. We perform a validation on 30 modern European sites and then apply the method to the sediment core of Meerfelder Maar (Germany), which covers the Holocene at a temporal resolution of approximately one sample per 30 years. We demonstrate that reconstructed temperatures are constrained. The reconstructed precipitation is less well constrained, due to the dimension considered (one precipitation by season), and the low sensitivity of LPJ-GUESS to precipitation changes. (orig.)

Vegetation carbon sequestration in Chinese forests from 2010 to 2050.

Science.gov (United States)

He, Nianpeng; Wen, Ding; Zhu, Jianxing; Tang, Xuli; Xu, Li; Zhang, Li; Hu, Huifeng; Huang, Mei; Yu, Guirui

2017-04-01

Forests store a large part of the terrestrial vegetation carbon (C) and have high C sequestration potential. Here, we developed a new forest C sequestration (FCS) model based on the secondary succession theory, to estimate vegetation C sequestration capacity in China's forest vegetation. The model used the field measurement data of 3161 forest plots and three future climate scenarios. The results showed that logistic equations provided a good fit for vegetation biomass with forest age in natural and planted forests. The FCS model has been verified with forest biomass data, and model uncertainty is discussed. The increment of vegetation C storage in China's forest vegetation from 2010 to 2050 was estimated as 13.92 Pg C, while the average vegetation C sequestration rate was 0.34 Pg C yr -1 with a 95% confidence interval of 0.28-0.42 Pg C yr -1 , which differed significantly between forest types. The largest contributor to the increment was deciduous broadleaf forest (37.8%), while the smallest was deciduous needleleaf forest (2.7%). The vegetation C sequestration rate might reach its maximum around 2020, although vegetation C storage increases continually. It is estimated that vegetation C sequestration might offset 6-8% of China's future emissions. Furthermore, there was a significant negative relationship between vegetation C sequestration rate and C emission rate in different provinces of China, suggesting that developed provinces might need to compensate for undeveloped provinces through C trade. Our findings will provide valuable guidelines to policymakers for designing afforestation strategies and forest C trade in China. © 2016 John Wiley & Sons Ltd.

An analysis on vegetation pattern of ecotone in North China

Energy Technology Data Exchange (ETDEWEB)

Jia, J.C.; Zhang, H.Y. [North China Electric Power Univ., Beijing (China). Energy and Environmental Research Center

2008-07-01

Vegetation pattern is influenced by several natural factors, including climatic elements, elevation factors and soil conditions. Since soil formation and soil types are influenced by water-temperature conditions, much can be learned about vegetation distribution patterns by studying the relationship between water-temperature conditions and vegetation distribution. This paper presented the results of a study whose purpose was to provide scientific evidence for exploiting natural resources, planting trees, and restoring grassland from cropland. A warmth index (WI ) and humidity index (HI) were used to examine the relation between the distribution of vegetation and the water-temperature condition in North China's ecotone, the transition area between two adjacent but different plant communities, including steppe, bush and forest ecosystems. A vegetation map of the study site was digitized and then converted into a vegetation grid map from which 17 different vegetation types were chosen as the study object. A monthly mean temperature grid map and precipitation grid map of the study site were made based on the method of spatial interpolation, by using 119 meteorological data for 50 years during the period from 1951 to 2000. The thermal distribution curves and humidity distribution curves of 17 vegetation types in North China, determined the whole range and optimum range of WI and HI of 17 vegetation types. The relative proportion of each vegetation type distributed in the optimum range of WI and HI were calculated. The vegetation pattern was analyzed according to the WI and HI standard, and was described by species and their relative amount. 10 refs., 5 tabs., 3 figs.

The role of vegetation in shaping dune morphology

Science.gov (United States)

Duran Vinent, O.; Moore, L. J.; Young, D.

2012-12-01

Aeolian dunes naturally emerge under strong winds and sufficient sand supply. They represent the most dynamical feature of the arid and/or coastal landscape and their evolution has the potential to either increase desertification or reduce coastal vulnerability to storms. Although large-scale dune morphology mainly depends on the wind regime and sand availability, vegetation plays an important role in semiarid and/or coastal areas. It is well known that under certain conditions vegetation is able to stabilize dunes, driving a morphological transformation from un-vegetated mobile crescent dunes to static vegetated "parabolic" dunes, de facto paralyzing desertification and initiating land recovery. Furthermore, vegetation is also the primary ingredient in the formation of coastal foredunes, which determine vulnerability to storms, as low dunes are prone to storm-induced erosion and overwash. In both cases, the coupling of biological and geomorphic (physical) processes, in particular vegetation growth and sand transport, governs the evolution of morphology. These processes were implemented in a computational model as part of a previous effort. It was shown that, for a migrating dune, this coupling leads to a negative feedback for dune motion, where an ever denser vegetation implies ever lesser sand transport. The model also predicted the existence of a "mobility index", defined by the vegetation growth rate to sand erosion rate ratio, that fully characterizes the morphological outcome: for indices above a certain threshold biological processes are dominant and dune motion slows after being covered by plants; for lower indices, the physical processes are the dominant ones and the dune remains mobile while vegetation is buried or rooted out. Here, we extend this model to better understand the formation of coastal dunes. We include new physical elements such as the shoreline and water table, as well as different grass species and potential competition among them

Assessment of nutritional quality of cooked Swazi leafy vegetables ...

African Journals Online (AJOL)

Brassica oleracea and Spinacia oleracea were also analysed and compared with other vegetables. Sampled vegetables were divided into two categories. All vegetables in category one were cooked without any additives. In category two Hibiscus escolentus and Corchorius olitorus were cooked with bicarbonate of soda ...

Vegetative regeneration

Science.gov (United States)

George A. Schier; John R. Jones; Robert P. Winokur

1985-01-01

Aspen is noted for its ability to regenerate vegetatively by adventitious shoots or suckers that arise on its long lateral roots. It also produces sprouts from stumps and root collars; but they are not common. In a survey of regeneration after clearcutting mature aspen in Utah. Baker (1918b) found that 92% of the shoots originated from roots, 7% from root collars, and...

Incineration of Low Level Radioactive Vegetation for Waste Volume Reduction

International Nuclear Information System (INIS)

Malik, N.P.S.; Rucker, G.G.; Looper, M.G.

1995-01-01

The DOE changing mission at Savannah River Site (SRS) are to increase activities for Waste Management and Environmental Restoration. There are a number of Comprehensive Environmental Response, Compensation and Liability Act (CERCLA) locations that are contaminated with radioactivity and support dense vegetation, and are targeted for remediation. Two such locations have been studied for non-time critical removal actions under the National Contingency Plan (NCP). Both of these sites support about 23 plant species. Surveys of the vegetation show that radiation emanates mainly from vines, shrubs, and trees and range from 20,000 to 200,000 d/m beta gamma. Planning for removal and disposal of low-level radioactive vegetation was done with two principal goals: to process contaminated vegetation for optimum volume reduction and waste minimization, and for the protection of human health and environment. Four alternatives were identified as candidates for vegetation removal and disposal: chipping the vegetation and packing in carbon steel boxes (lined with synthetic commercial liners) and disposal at the Solid Waste Disposal Facility at SRS; composting the vegetation; burning the vegetation in the field; and incinerating the vegetation. One alternative 'incineration' was considered viable choice for waste minimization, safe handling, and the protection of the environment and human health. Advantages and disadvantages of all four alternatives considered have been evaluated. For waste minimization and ultimate disposal of radioactive vegetation incineration is the preferred option. Advantages of incineration are that volume reduction is achieved and low-level radioactive waste are stabilized. For incineration and final disposal vegetation will be chipped and packed in card board boxes and discharged to the rotary kiln of the incinerator. The slow rotation and longer resident time in the kiln will ensure complete combustion of the vegetative material

Survival, growth, and body residues of hyalella azteca (Saussure) exposed to fipronil contaminated sediments from non-vegetated and vegetated microcosms.

Science.gov (United States)

Kröger, Robert; Lizotte, Richard E; Moore, Matthew T

2009-09-01

We assessed chronic effects of fipronil and metabolite contaminated sediments from non-vegetated and Thallia dealbata vegetated wetland microcosms on Hyalella azteca during wet and dry exposures. Mean sediment concentrations (ng g(-1)) ranged from 0.72-1.26, 0.01-0.69, 0.07-0.23, and 0.49-7.87 for fipronil, fipronil-sulfide, fipronil-sulfone, and fipronil-desulfinyl, respectively. No significant differences in animal survival or growth were observed between non-vegetated and vegetated microcosms during wet or dry exposures. Mean animal body residue concentrations (ng g(-1)) ranged from 28.4-77.6, 0-30.7, and 8.3-43.8 for fipronil, fipronil-sulfide, and fipronil-sulfone. Fipronil-desulfinyl was not detected in any animal samples.

Climate change and spatial distribution of vegetation in Colombia

Directory of Open Access Journals (Sweden)

Juan Carlos Alarcon Hincapie

2013-12-01

Full Text Available Vegetation change under two climate change scenarios in different periods of the 21st Century are modeled for Colombia. Vegetation for the years 1970 to 2000 was reproduced using the Holdridge model with climate data with a spatial resolution of 900 meters. The vegetation types that occupied the most territory were sub-humid tropical forest, tropical dry forest and Andean wet forest. These results were validated by comparing with the Colombian ecosystem map (SINA, 2007, which confirmed a high degree of similarity between the modeled spatial vegetation patterns and modern ecosystem distributions. Future vegetation maps were simulated using data generated by a regional climate model under two scenarios (A2 and B2; IPCC, 2007 for the periods 2011-2040 and 2070-2100. Based on our predictions high altitude vegetation will convert to that of lower altitudes and drier provinces with the most dramatic change occurring in the A2 scenario from 2070-2100. The most affected areas are the páramo and other high Andean vegetation types, which in the timeframe of the explored scenarios will disappear by the middle of the 21st Century.

Snow effects on alpine vegetation in the Qinghai-Tibetan Plateau

Science.gov (United States)

Wang, Kun; Zhang, Li; Qiu, Yubao; Ji, Lei; Tian, Feng; Wang, Cuizhen; Wang, Zhiyong

2013-01-01

Understanding the relationships between snow and vegetation is important for interpretation of the responses of alpine ecosystems to climate changes. The Qinghai-Tibetan Plateau is regarded as an ideal area due to its undisturbed features with low population and relatively high snow cover. We used 500 m Moderate Resolution Imaging Spectroradiometer (MODIS) datasets during 2001–2010 to examine the snow–vegetation relationships, specifically, (1) the influence of snow melting date on vegetation green-up date and (2) the effects of snow cover duration on vegetation greenness. The results showed that the alpine vegetation responded strongly to snow phenology (i.e., snow melting date and snow cover duration) over large areas of the Qinghai-Tibetan Plateau. Snow melting date and vegetation green-up date were significantly correlated (p growth was influenced by different seasonal snow cover durations (SCDs) in different regions. Generally, the December–February and March–May SCDs played a significantly role in vegetation growth, both positively and negatively, depending on different water source regions. Snow's positive impact on vegetation was larger than the negative impact.

Improvement in remote sensing of low vegetation cover in arid regions by correcting vegetation indices for soil ''noise''

International Nuclear Information System (INIS)

Escadafal, R.; Huete, A.

1991-01-01

The variations of near-infrared red reflectance ratios of ten aridic soil samples were correlated with a ''redness index'' computed from red and green spectral bands. These variations have been shown to limit the performances of vegetation indices (NDVI and SAVI) in discriminating low vegetation covers. The redness index is used to adjust for this ''soil noise''. Dala simulated for vegetation densities of 5 to 15% cover showed that the sensitivity of the corrected vegetation indices was significantly improved. Specifically, the ''noise-corrected'' SAVI was able to assess vegetation amounts with an error four times smaller than the uncorrected NDVI. These promising results should lead to a significant improvement in assessing biomass in arid lands from remotely sensed data. (author) [fr

Risk elements in selected types of vegetables

Directory of Open Access Journals (Sweden)

Ľuboš Harangozo

2016-12-01

Full Text Available Vegetable has an important role in human nutrition. Various parts of the plants have been part of the human diet since the beginning. Vegetables have a number of properties that make its consumption very healthful. It not only is a good source of vitamins, minerals and fiber but also contains protective components so called phytonutrients, has an antioxidant and antimicrobial effects. Daily intake of vegetables offers many health benefits, helps to improve health for example the function of digestive and immune system, reduces the risk of various diseases and so we should take care to its regular consumption. It is widely used, except that it is the basic raw material for the preparation of foods and is also an important raw material for the processing industry. Nowadays has become environmental pollution by heavy metals as a big problem. The contamination of water, soil as well as air pollution by heavy metals negatively affects agricultural production and production of non-harmful to health, safe and quality food, which may be adverse effects on human health. Therefore, it is important that we devote this issue more attention. The aim of this work was to identify and determine content of heavy metals in selected vegetables. Defined objectives have been achieved by analyzing of selected species samples of root from brassica vegetables: carrot (Daucus carota L. ssp. sativus, parsley (Petroselinum hortesne HOFFM conv. radicosum, kohlrabi (Brassica oleracea L. var. gongylodes, celery (Apium graveolens L. var. rapaceum and beetroot (Beta vulgaris L. var. conditiva ssp. vulgaris. The crops were bought in local market. The obtained results were compared with the results obtained from analyzes of vegetables that were grown in home conditions respectively from markets of local growers. All crops were grown in Slovak Republic. By using Varian AA 240FS and AAS method were analyzed the contents of risk metals in selected vegetables. It was confirmed that

The vegetation of Yucca Mountain: Description and ecology

Energy Technology Data Exchange (ETDEWEB)

NONE

1996-03-29

Vegetation at Yucca Mountain, Nevada, was monitored over a six-year period, from 1989 through 1994. Yucca Mountain is located at the northern limit of the Mojave Desert and is the only location being studied as a potential repository for high-level nuclear waste. Site characterization consists of a series of multidisciplinary, scientific investigations designed to provide detailed information necessary to assess the suitability of the Yucca Mountain Site as a repository. This vegetation description establishes a baseline for determining the ecological impact of site characterization activities; it porvides input for site characterization research and modeling; and it clarifies vegetation community dynamics and relationships to the physical environment. A companion study will describe the impact of site characterization of vegetation. Cover, density, production, and species composition of vascular plants were monitored at 48 Ecological Study Plots (ESPs) stratified in four vegetation associations. Precipitation, soil moisture, and maximum and minimum temperatures also were measured at each study plot.

The vegetation of Yucca Mountain: Description and ecology

International Nuclear Information System (INIS)

1996-01-01

Vegetation at Yucca Mountain, Nevada, was monitored over a six-year period, from 1989 through 1994. Yucca Mountain is located at the northern limit of the Mojave Desert and is the only location being studied as a potential repository for high-level nuclear waste. Site characterization consists of a series of multidisciplinary, scientific investigations designed to provide detailed information necessary to assess the suitability of the Yucca Mountain Site as a repository. This vegetation description establishes a baseline for determining the ecological impact of site characterization activities; it porvides input for site characterization research and modeling; and it clarifies vegetation community dynamics and relationships to the physical environment. A companion study will describe the impact of site characterization of vegetation. Cover, density, production, and species composition of vascular plants were monitored at 48 Ecological Study Plots (ESPs) stratified in four vegetation associations. Precipitation, soil moisture, and maximum and minimum temperatures also were measured at each study plot

Effects of aquatic vegetation type on denitrification

NARCIS (Netherlands)

Veraart, A.J.; Bruijne, de W.J.J.; Peeters, E.T.H.M.; Klein, de J.J.M.; Scheffer, M.

2011-01-01

In a microcosm 15N enrichment experiment we tested the effect of floating vegetation (Lemna sp.) and submerged vegetation (Elodea nuttallii) on denitrification rates, and compared it to systems without macrophytes. Oxygen concentration, and thus photosynthesis, plays an important role in regulating

Understanding and changing children’s sensory acceptance for vegetables

NARCIS (Netherlands)

Poelman, A.A.M.

2016-01-01

Vegetable intake of children is well below recommendations in Australia and in most other western countries. Vegetables are the food category least liked by children. As acceptance is a key driver of intake, strategies are needed to increase children’s acceptance of vegetables. The present thesis

The contribution of vegetation to riverbed morphology (Invited)

Science.gov (United States)

Bertoldi, W.; Gurnell, A. M.

2010-12-01

The occurrence, form and species composition of riparian and aquatic vegetation are all strongly affected by the flow energy regime, sediment calibre and dimensions of river systems. In this paper, we build on field examples to conceptualise how interactions between vegetation and fluvial processes may affect river form across a gradient of river types from high-energy gravel-bed braided rivers to lowland single-thread silt-bed rivers. We explore how different vegetation types (e.g. riparian trees, shrubs, emergent macrophytes), and in some cases particular plant species, can produce similar impacts on the bed topography of rivers of different size, because of their effect on sediment transport flux and sediment cohesion, and a resultant positive feedback that increases the bar or bank height. We illustrate these concepts using two case studies representing extremes of river size and energy. Field and remotely sensed data are used to identify and quantify impacts of vegetation density on the bed morphology of the >1km wide, gravel-bed, braided Tagliamento River (Italy). Analysis of airborne LiDAR data is used to compute a highly detailed digital elevation model, along with data on tree height and density. The comparison between reaches with different tree height and density clearly shows the active role of vegetation in determining river pattern and form, with tree growth rate being the main parameter determining the vegetation effect. Analysis of field measurements of flow patterns and mechanical properties of emergent aquatic macrophytes on the <10m wide, silt-bed, single-thread River Blackwater (England) illustrate the close correspondence of the bed topography with vegetation structures, with position along an energy gradient dictating changes in the structure of the vegetation-bed morphology interaction.

Dietary fruits and vegetables and cardiovascular diseases risk.

Science.gov (United States)

Alissa, Eman M; Ferns, Gordon A

2017-06-13

Diet is likely to be an important determinant of cardiovascular disease (CVD) risk. In this article, we will review the evidence linking the consumption of fruit and vegetables and CVD risk. The initial evidence that fruit and vegetable consumption has a protective effect against CVD came from observational studies. However, uncertainty remains about the magnitude of the benefit of fruit and vegetable intake on the occurrence of CVD and whether the optimal intake is five portions or greater. Results from randomized controlled trials do not show conclusively that fruit and vegetable intake protects against CVD, in part because the dietary interventions have been of limited intensity to enable optimal analysis of their putative effects. The protective mechanisms of fruit and vegetables may not only include some of the known bioactive nutrient effects dependent on their antioxidant, anti-inflammatory, and electrolyte properties, but also include their functional properties, such as low glycemic load and energy density. Taken together, the totality of the evidence accumulated so far does appear to support the notion that increased intake of fruits and vegetables may reduce cardiovascular risk. It is clear that fruit and vegetables should be eaten as part of a balanced diet, as a source of vitamins, fiber, minerals, and phytochemicals. The evidence now suggests that a complicated set of several nutrients may interact with genetic factors to influence CVD risk. Therefore, it may be more important to focus on whole foods and dietary patterns rather than individual nutrients to successfully impact on CVD risk reduction. A clearer understanding of the relationship between fruit and vegetable intake and cardiovascular risk would provide health professionals with significant information in terms of public health and clinical practice.

Attribution of trends in global vegetation greenness from 1982 to 2011

Science.gov (United States)

Zhu, Z.; Xu, L.; Bi, J.; Myneni, R.; Knyazikhin, Y.

2012-12-01

Time series of remotely sensed vegetation indices data provide evidence of changes in terrestrial vegetation activity over the past decades in the world. However, it is difficult to attribute cause-and-effect to vegetation trends because variations in vegetation productivity are driven by various factors. This study investigated changes in global vegetation productivity first, and then attributed the global natural vegetation with greening trend. Growing season integrated normalized difference vegetation index (GSI NDVI) derived from the new GIMMS NDVI3g dataset (1982-2011was analyzed. A combined time series analysis model, which was developed from simper linear trend model (SLT), autoregressive integrated moving average model (ARIMA) and Vogelsang's t-PST model shows that productivity of all vegetation types except deciduous broadleaf forest predominantly showed increasing trends through the 30-year period. The evolution of changes in productivity in the last decade was also investigated. Area of greening vegetation monotonically increased through the last decade, and both the browning and no change area monotonically decreased. To attribute the predominant increase trend of productivity of global natural vegetation, trends of eight climate time series datasets (three temperature, three precipitation and two radiation datasets) were analyzed. The attribution of trends in global vegetation greenness was summarized as relaxation of climatic constraints, fertilization and other unknown reasons. Result shows that nearly all the productivity increase of global natural vegetation was driven by relaxation of climatic constraints and fertilization, which play equally important role in driving global vegetation greenness.; Area fraction and productivity change fraction of IGBP vegetation land cover classes showing statistically significant (10% level) trend in GSI NDVIt;

Be-7 concentration in garden and wild vegetables in Japan

International Nuclear Information System (INIS)

Nobuhiko Ito; Iwao Kunugijama; Yoshinori Furukawa.

1996-01-01

Beryllium-7 is a natural radionuclide produced by cosmic rays. Be-7 is found vividly in the atmosphere, so the concentrations in airborne particles were analyzed by many investigators. It is known that airborne particles with Be-7 in the atmosphere fall slowly to the ground and adhere to plant surface. However, Be-7 concentrations of foods were not measured too much. So we measured Be-7 concentrations of garden vegetables, wild vegetables, grasses, beef and milk, and calculated internal exposure dose from Be-7. Beryllium-7 concentrations of plant samples are shown. Beryllium-7 concentrations of garden vegetables were from 0.2 to 25.3 Bq/kg, and concentrations of wild vegetables were from 0.8 to 23.5 Bq/kg. There is no difference in Be-7 concentrations between garden vegetables and wild vegetables. Leaf vegetables have almost higher concentration of Be-7. Though Matteuccia Struthiopteris and Pteridium aquilinum are ferns, their eatable stages are sprouts. The relationship between surface area and Be-7 concentration in some vegetable leaves gathered simultaneously at the same farm is shown. Beryllium-7 concentrations of leaves correlate significantly with the surface area/weight ratios. What high concentration vegetables have proportionately broad leaves suggests that atmospheric Be-7 particles adhere to surface of leaves. (author)

Effects of Vegetables on Cardiovascular Diseases and Related Mechanisms

Directory of Open Access Journals (Sweden)

Guo-Yi Tang

2017-08-01

Full Text Available Epidemiological studies have shown that vegetable consumption is inversely related to the risk of cardiovascular diseases. Moreover, research has indicated that many vegetables like potatoes, soybeans, sesame, tomatoes, dioscorea, onions, celery, broccoli, lettuce and asparagus showed great potential in preventing and treating cardiovascular diseases, and vitamins, essential elements, dietary fibers, botanic proteins and phytochemicals were bioactive components. The cardioprotective effects of vegetables might involve antioxidation; anti-inflammation; anti-platelet; regulating blood pressure, blood glucose, and lipid profile; attenuating myocardial damage; and modulating relevant enzyme activities, gene expression, and signaling pathways as well as some other biomarkers associated to cardiovascular diseases. In addition, several vegetables and their bioactive components have been proven to protect against cardiovascular diseases in clinical trials. In this review, we analyze and summarize the effects of vegetables on cardiovascular diseases based on epidemiological studies, experimental research, and clinical trials, which are significant to the application of vegetables in prevention and treatment of cardiovascular diseases.

Map of mixed prairie grassland vegetation, Rocky Flats, Colorado

Energy Technology Data Exchange (ETDEWEB)

Clark, S J.V.; Webber, P J; Komarkova, V; Weber, W A

1980-01-01

A color vegetation map at the scale of 1:12,000 of the area surrounding the Rocky Flats, Rockwell International Plant near Boulder, Colorado, provides a permanent record of baseline data which can be used to monitor changes in both vegetation and environment and thus to contribute to future land management and land-use policies. Sixteen mapping units based on species composition were identified, and characterized by two 10-m/sup 2/ vegetation stands each. These were grouped into prairie, pasture, and valley side on the basis of their species composition. Both the mapping units and these major groups were later confirmed by agglomerative clustering analysis of the 32 vegetation stands on the basis of species composition. A modified Bray and Curtis ordination was used to determine the environmental factor complexes controlling the distribution of vegetation at Rocky flats. Recommendations are made for future policies of environmental management and predictions of the response to environmental change of the present vegetation at the Rocky Flats site.

Occurrence and source analysis of typical veterinary antibiotics in manure, soil, vegetables and groundwater from organic vegetable bases, northern China

International Nuclear Information System (INIS)

Hu Xiangang; Zhou Qixing; Luo Yi

2010-01-01

The residue of antibiotics is becoming an intractable environmental problem in many organic vegetable bases. However, their residual levels and distribution are still obscure. This work systematically analyzed the occurrence and migration of typical veterinary antibiotics in organic vegetable bases, northern China. The results showed that there was no obvious geographical difference in antibiotic distribution between soil and manure. A simple migration model can be easy and quick to predict the accumulation of antibiotics in soil. Antibiotics were mainly taken up through water transport and passive absorption in vegetables. The distribution of antibiotics in a plant was in the sequence leaf > stem > root, and performed biological accumulation. The residues of antibiotics in all samples in winter were significantly higher than those in summer. Overall, this work can lay the foundation for understanding ecological risk of antibiotics and their potential adverse effects on human health by food chain. - The residues of typical veterinary antibiotics from manure were detected and migrated in soil, vegetables and groundwater of organic vegetable bases.

Effects of Warming Hiatuses on Vegetation Growth in the Northern Hemisphere

Directory of Open Access Journals (Sweden)

Hong Wei

2018-04-01

Full Text Available There have been hiatuses in global warming since the 1990s, and their potential impacts have attracted extensive attention and discussion. Changes in temperature not only directly affect the greening of vegetation but can also indirectly alter both the growth state and the growth tendency of vegetation by altering other climatic elements. The middle-high latitudes of the Northern Hemisphere (NH constitute the region that has experienced the most warming in recent decades; therefore, identifying the effects of warming hiatuses on the vegetation greening in that region is of great importance. Using satellite-derived Normalized Difference Vegetation Index (NDVI data and climatological observation data from 1982–2013, we investigated hiatuses in warming trends and their impact on vegetation greenness in the NH. Our results show that the regions with warming hiatuses in the NH accounted for 50.1% of the total area and were concentrated in Mongolia, central China, and other areas. Among these regions, 18.8% of the vegetation greenness was inhibited in the warming hiatus areas, but 31.3% of the vegetation grew faster. Because temperature was the main positive climatic factor in central China, the warming hiatuses caused the slow vegetation greening rate. However, precipitation was the main positive climatic factor affecting vegetation greenness in Mongolia; an increase in precipitation accelerated vegetation greening. The regions without a warming hiatus, which were mainly distributed in northern Russia, northern central Asia, and other areas, accounted for 49.9% of the total area. Among these regions, 21.4% of the vegetation grew faster over time, but 28.5% of the vegetation was inhibited. Temperature was the main positive factor affecting vegetation greenness in northern Russia; an increase in temperature promoted vegetation greening. However, radiation was the main positive climatic factor in northern central Asia; reductions in radiation

Abrupt vegetation transitions characterise long-term Amazonian peatland development

Science.gov (United States)

Roucoux, K. H.; Baker, T. R.; Gosling, W. D.; Honorio Coronado, E.; Jones, T. D.; Lahteenoja, O.; Lawson, I. T.

2012-04-01

Recent investigations of wetlands in western Amazonia have revealed the presence of extensive peatlands with peat deposits of up to 8 m-thick developing under a variety of vegetation types (Lähteenoja et al. 2012). Estimated to cover 150,000 km2 (Schulman et al. 1999), these peatlands make a valuable contribution to landscape and biological diversity and represent globally important carbon stores. In order to understand the processes leading to peat formation, and the sensitivity of these environments to future climatic change, it is necessary to understand their long-term history. The extent to which peatland vegetation changes over time, the stability of particular communities, the controls on transitions between vegetation types and how these factors relate to the accumulation of organic matter are not yet known. We report the first attempt to establish the long-term (millennial scale) vegetation history of a recently-described peatland site: Quistococha, a palm swamp, or aguajal, close to Iquitos in northern Peru. The vegetation is dominated by Mauritia flexuosa and Mauritiella armata and occupies a basin which is thought to be an abandoned channel of the River Amazon. We obtained a 4 m-long peat sequence from the deepest part of the basin. AMS-radiocarbon dating yielded a maximum age of 2,212 cal yr BP for the base of the peat, giving an average accumulation rate of 18 cm per century. Below the peat are 2 m of uniform, largely inorganic pale grey clays of lacustrine origin, which are underlain by an unknown thickness of inorganic sandy-silty clay of fluvial origin. Pollen analysis, carried out at c. 88-year intervals, shows the last 2,212 years to be characterised by the development of at least four distinct vegetation communities, with peat accumulating throughout. The main phases were: (1) Formation of Cyperaceae (sedge) fen coincident with peat initiation; (2) A short-lived phase of local Mauritia/Mauritiella development; (3) Development of mixed wet

Field vegetable production in the Lake Zone of Tanzania

NARCIS (Netherlands)

Everaarts, A.P.; Putter, de H.; Maerere, A.P.; Amon, W.

2014-01-01

In November 2012 and in August 2014 surveys were carried out in field vegetable producing areas in the Lake Zone of Tanzania. The aim of the surveys was to learn the conditions for field vegetable production and marketing in these areas. Recommendations for the development of vegetable production

Production and action pattern of inulinase from Aspergillus Niger-245: hydrolysis of inulin from several sources

Directory of Open Access Journals (Sweden)

Cruz Vinícius D?Arcadia

1998-01-01

Full Text Available A strain of Aspergillus niger isolated from soil samples showed great capacity to produce extracellular inulinase. Although the enzyme has been synthesized in presence of monosaccharides, sucrose and sugar cane molasse, the productivity was significantly higher (p<0.05 when the microorganism was inoculated in media formulated with dahlia extract and pure inulin, as carbon sources. With regard to the nitrogen source, the best results were obtained with casein and other sources of proteic nitrogen, comparatively to the mineral nitrogen. However, statistic significance (p<0.01 only was found between the productivity obtained in the medium prepared with casein and ammonium sulphate. The optimum pH of the purified enzyme for inulin hydrolysis was found between 4.0 and 4.5 and the optimun temperature at 60oC. When treated by 30 minutes in this temperature no loss of activity was observed. The enzyme showed capacity to hydrolyse sucrose, raffinose and inulin from which it liberated only fructose units showing, therefore, an exo-action mechanism. Acting on inulins from several sources, the enzyme showed larger hydrolysis speed on the polissaccharide from chicory (Cichorium intibus, comparatively, to the inulins from dahlia (Dahlia pinnata and Jerusalem artichoke (Helianthus tuberosus roots.

Golden Gate National Recreation Area Vegetation Inventory Project

Data.gov (United States)

California Natural Resource Agency — High resolution vegetation polygons mapped by the National Park Service. The vegetation units of this map were determined through stereoscopic interpretation of...

Health-promoting components of fruits and vegetables in the diet.

Science.gov (United States)

Liu, Rui Hai

2013-05-01

Regular consumption of fruits, vegetables, whole grains, and other plant foods has been negatively correlated with the risk of the development of chronic diseases. There is a huge gap between the average consumption of fruits and vegetables in Americans and the amount recommended by the 2010 Dietary Guidelines for Americans. The key is to encourage consumers to increase the total amount to 9 to 13 servings of fruits and vegetables in all forms available. Fresh, processed fruits and vegetables including frozen and canned, cooked, 100% fruit juices and 100% vegetable juices, as well as dry fruits are all considered as servings of fruits and vegetables per day. A wide variety of fruits, vegetables, whole grains, and other plant foods provide a range of nutrients and different bioactive compounds including phytochemicals, vitamins, minerals, and fibers. Potatoes serve as one of the low-fat foods with unique nutrients and phytochemical profiles, particularly rich in vitamin C, vitamin B-6, potassium, manganese, and dietary fibers. Potatoes provide 25% of vegetable phenolics in the American diet, the largest contributors among the 27 vegetables commonly consumed in the United States, including flavonoids (quercetin and kaempferol), phenolic acids (chlorogenic acid and caffeic acid), and carotenoids (lutein and zeaxanthin). More and more evidence suggests that the health benefits of fruits, vegetables, whole grains, and other plant foods are attributed to the synergy or interactions of bioactive compounds and other nutrients in whole foods. Therefore, consumers should obtain their nutrients, antioxidants, bioactive compounds, and phytochemicals from a balanced diet with a wide variety of fruits, vegetables, whole grains, and other plant foods for optimal nutrition, health, and well-being, not from dietary supplements.

Effect of vegetation on rock and soil type discrimination

Science.gov (United States)

Siegal, B. S.; Goetz, A. F. H.

1977-01-01

The effect of naturally occurring vegetation on the spectral reflectance of earth materials in the wavelength region of 0.45 to 2.4 microns is determined by computer averaging of in situ acquired spectral data. The amount and type of vegetation and the spectral reflectance of the ground are considered. Low albedo materials may be altered beyond recognition with only ten per cent green vegetation cover. Dead or dry vegetation does not greatly alter the shape of the spectral reflectance curve and only changes the albedo with minimum wavelength dependency. With increasing amounts of vegetation the Landsat MSS band ratios 4/6, 4/7, 5/6, and 5/7 are significantly decreased whereas MSS ratios 4/5 and 6/7 remain entirely constant.

Biodiversity of Terrestrial Vegetation during Past Warm Periods

Science.gov (United States)

Davies-Barnard, T.; Valdes, P. J.; Ridgwell, A.

2016-12-01

Previous modelling studies of vegetation have generally used a small number of plant functional types to understand how the terrestrial biosphere responds to climate changes. Whilst being useful for understanding first order climate feedbacks, this climate-envelope approach makes a lot of assumptions about past vegetation being very similar to modern. A trait-based method has the advantage for paleo modelling in that there are substantially less assumptions made. In a novel use of the trait-based dynamic vegetation model JeDi, forced with output from climate model HadCM3, we explore past biodiversity and vegetation carbon changes. We use JeDi to model an optimal 2000 combinations of fifteen different traits to enable assessment of the overall level of biodiversity as well as individual growth strategies. We assess the vegetation shifts and biodiversity changes in past greenhouse periods to better understand the impact on the terrestrial biosphere. This work provides original insights into the response of vegetation and terrestrial carbon to climate and hydrological changes in high carbon dioxide climates over time, including during the Late Permian and Cretaceous. We evaluate how the location of biodiversity hotspots and species richness in past greenhouse climates is different to the present day.

Food design strategies to increase vegetable intake

NARCIS (Netherlands)

Oliviero, Teresa; Fogliano, Vincenzo

2016-01-01

Background: Public campaigns promoting consumption of fruits and vegetables had limited results as consumers habits are difficult to modify. The incorporation of fruits and vegetables into regularly eaten products is a food design strategy that leads to several advantages. Pasta is a staple food

Use of Plastic Mulch for Vegetable Production

OpenAIRE

Maughan, Tiffany; Drost, Dan

2016-01-01

Plastic mulches are used commercially for both vegetables and small fruit crops. Vegetable crops well suited for production with plastic mulch are typically high value row crops. This fact sheet describes the advantages, disadvantages, installation, and planting considerations. It includes sources for plastic and equipment.

STOVE: Seed treatments for organic vegetable production

NARCIS (Netherlands)

Schmitt, A.; Jahn, M.; Kromphardt, C.; Krauthausen, H.J.; Roberts, S.J.; Wright, S.A.I.; Amein, T.; Forsberg, G.; Tinivella, F.; Gullino, M.L.; Wikström, M.; Wolf, van der J.M.; Groot, S.P.C.; Werner, S.; Koch, E.

2008-01-01

The aim of the EU-financed research project „STOVE“ (Seed Treatments for Organic Vegetable Production) is to evaluate different methods potentially suited for seed treatment of vegetables in organic farming regarding their efficacy, to optimise these methods, and where feasible to combine them with

Analysis of vegetation recovery surrounding a restored wetland using the normalized difference infrared index (NDII) and normalized difference vegetation index (NDVI)

Science.gov (United States)

Wilson, Natalie R.; Norman, Laura

2018-01-01

Watershed restoration efforts seek to rejuvenate vegetation, biological diversity, and land productivity at Cienega San Bernardino, an important wetland in southeastern Arizona and northern Sonora, Mexico. Rock detention and earthen berm structures were built on the Cienega San Bernardino over the course of four decades, beginning in 1984 and continuing to the present. Previous research findings show that restoration supports and even increases vegetation health despite ongoing drought conditions in this arid watershed. However, the extent of restoration impacts is still unknown despite qualitative observations of improvement in surrounding vegetation amount and vigor. We analyzed spatial and temporal trends in vegetation greenness and soil moisture by applying the normalized difference vegetation index (NDVI) and normalized difference infrared index (NDII) to one dry summer season Landsat path/row from 1984 to 2016. The study area was divided into zones and spectral data for each zone was analyzed and compared with precipitation record using statistical measures including linear regression, Mann– Kendall test, and linear correlation. NDVI and NDII performed differently due to the presence of continued grazing and the effects of grazing on canopy cover; NDVI was better able to track changes in vegetation in areas without grazing while NDII was better at tracking changes in areas with continued grazing. Restoration impacts display higher greenness and vegetation water content levels, greater increases in greenness and water content through time, and a decoupling of vegetation greenness and water content from spring precipitation when compared to control sites in nearby tributary and upland areas. Our results confirm the potential of erosion control structures to affect areas up to 5 km downstream of restoration sites over time and to affect 1 km upstream of the sites.

Contributions of seed bank and vegetative propagules to vegetation composition on prairie dog colonies in western South Dakota

Science.gov (United States)

Emily R. Helms; Lan Xu; Jack L. Butler

2012-01-01

Characterizing the contributions of the seed bank and vegetative propagules will enhance our understanding of community resiliency associated with prairie dog disturbances. Our objective was to determine the effects of ecological condition (EC) and distance from burrows on the soil seed bank and vegetative propagules. Based on species composition of the extant...

Influence of seasoning on vegetable selection, liking and intent to purchase.

Science.gov (United States)

Manero, Joanna; Phillips, Carter; Ellison, Brenna; Lee, Soo-Yeun; Nickols-Richardson, Sharon M; Chapman-Novakofski, Karen M

2017-09-01

Low vegetable intake continues to be a health concern, and strategies to increase vegetable intake have resulted in only small increases. One strategy that has received less attention is the use of seasonings. This study's objective was to determine the impact of seasoning on vegetable selection, liking, and intent to purchase. We conducted a 3-week study in a public café on a university campus. Customers buying a main dish could select a vegetable side (seasoned [SS] or steamed [ST]) at no cost. Based on café data and power analysis (alpha 0.05, 80% power), 2 days per vegetable pair were conducted with carrot, broccoli, and green bean pairs randomized 3 days/week 1 and 3, with normal service week 2. Selection was greater for SS vs ST, n = 335 vs. 143 for all 3 vegetables combined; n = 97 vs 47 for carrots; n = 114 vs. 55 for broccoli; n = 124 vs. 41 for green beans (p purchase the vegetable that they selected. More customers chose the 'somewhat likely' and 'very likely' (n = 353) than the 'not likely' and 'definitely would not' (n = 121) purchase responses. Regression showed that people who did not often consume a vegetable with lunch while dining out were 1.59 times more likely to select the SS vegetables over the ST (p = 0.007). Given a choice, consumers were more likely to select a seasoned vegetable. With low vegetable consumption as a predictor of seasoned vegetable choice, offering seasoned vegetables may increase intake in those with poor vegetable intake in a café setting. Copyright © 2017 The Authors. Published by Elsevier Ltd.. All rights reserved.

Nutrient Density and the Cost of Vegetables from Elementary School Lunches.

Science.gov (United States)

Ishdorj, Ariun; Capps, Oral; Murano, Peter S

2016-01-01

Vegetables are the major source of the dietary fiber, magnesium, potassium, and vitamins A and C that are crucial in the diets of children. This study assessed the nutrient content of vegetables offered through the National School Lunch Program and examined the relation between the overall nutrient density of vegetable subgroups and the costs of nutrients offered and wasted before and after the changes in school meal standards. Using data collected from 3 elementary schools before and after the changes in school meal standards, we found that vegetable plate waste increased from 52% to 58%. Plate waste for starchy vegetables, exclusive of potatoes, was relatively high compared with other subgroups; however, plate waste for white potatoes was the lowest among any type of vegetable. Energy density; cost per 100 g, per serving, and per 100 kcal; and percentage daily value were calculated and used to estimate nutrient density value and nutrient density per dollar. Cost per 100 kcal was highest for red/orange vegetables followed by dark green vegetables; however, nutrient density for red/orange vegetables was the highest in the group and provided the most nutrients per dollar compared with other subgroups. Given that many vegetables are less energy dense, measuring vegetable costs per 100 g and per serving by accounting for nutrient density perhaps is a better way of calculating the cost of vegetables in school meals. © 2016 American Society for Nutrition.

[Progress in inversion of vegetation nitrogen concentration by hyperspectral remote sensing].

Science.gov (United States)

Wang, Li-Wen; Wei, Ya-Xing

2013-10-01

Nitrogen is the necessary element in life activity of vegetation, which takes important function in biosynthesis of protein, nucleic acid, chlorophyll, and enzyme etc, and plays a key role in vegetation photosynthesis. The technology about inversion of vegetation nitrogen concentration by hyperspectral remote sensing has been the research hotspot since the 70s of last century. With the development of hyperspectral remote sensing technology in recent years, the advantage of spectral bands subdivision in a certain spectral region provides the powerful technology measure for correlative spectral characteristic research on vegetation nitrogen. In the present paper, combined with the newest research production about monitoring vegetation nitrogen concentration by hyperspectral remote sensing published in main geography science literature in recent several years, the principle and correlated problem about monitoring vegetation nitrogen concentration by hyperspectral remote sensing were introduced. From four aspects including vegetation nitrogen spectral index, vegetation nitrogen content inversion based on chlorophyll index, regression model, and eliminating influence factors to inversion of vegetation nitrogen concentration, main technology methods about inversion of vegetation nitrogen concentration by hyperspectral remote sensing were detailedly introduced. Correlative research conclusions were summarized and analyzed, and research development trend was discussed.

SMAP Multi-Temporal Soil Moisture and Vegetation Optical Depth Retrievals in Vegetated Regions Including Higher-Order Soil-Canopy Interactions

Science.gov (United States)

Feldman, A.; Akbar, R.; Konings, A. G.; Piles, M.; Entekhabi, D.

2017-12-01

The Soil Moisture Active Passive (SMAP) mission utilizes a zeroth order radiative transfer model, known as the tau-omega model, to retrieve soil moisture from microwave brightness temperature observations. This model neglects first order scattering which is significant at L-Band in vegetated regions, or 30% of land cover. Previous higher order algorithms require extensive in-situ measurements and characterization of canopy layer physical properties. We propose a first order retrieval algorithm that approximately characterizes the eight first order emission pathways using rough surface reflectivity, vegetation optical depth (VOD), and scattering albedo terms. The recently developed Multi-Temporal Dual Channel Algorithm (MT-DCA) then retrieves these three parameters in a forward model without ancillary information under the assumption of temporally static albedo and constant vegetation water content between three day SMAP revisits. The approximated scattering terms are determined to be conservative estimates of analytically derived first order scattering terms. In addition, we find the first order algorithm to be more sensitive to surface emission than the tau-omega model. The simultaneously retrieved VOD, previously demonstrated to be proportional to vegetation water content, can provide insight into vegetation dynamics in regions with significant phenology. Specifically, dry tropical forests exhibit an increase in VOD during the dry season in alignment with prior studies that suggest that certain vegetative species green up during the dry season despite limited water availability. VOD retrieved using the first order algorithm and MT-DCA framework can therefore contribute to understanding of tropical forests' role in the carbon, energy, and water cycles, which has yet to be fully explained.

Genetic resources of cultivated and volunteer vegetables in Serbia

Directory of Open Access Journals (Sweden)

Lazić Branka

2017-01-01

Full Text Available Number of plant species and populations in the diet and agriculture, and knowledge about them, their cultivation and use, represent the biological, cultural and social heritage of the humankind. Due to favourable geographic, soil, and climate conditions, and large diversity of flora in Serbia, there is a rich tradition of vegetable use in the diet, cultivated and volunteer, autochthonous. More than 70 botanical vegetable species that are accepted for long-term preservation are mentioned nowadays in Serbia. Besides that, some plant species that are classified as industrial, medicinal and aromatic plants are being used as vegetables. Vegetables include legumes used as stewing vegetables. Serbia has three vegetable regions: lowlands (along the rivers Danube, Tisa and Sava, Moravian (along three Morava Rivers and their tributaries and mountainous (Eastern, South-eastern and Western areas, Kosovo and Metohija. In these regions, genetic resources of vegetables should be looked for in cultivated and volunteering flora of different domestic and domesticated populations developed due to adaptation to the specific abiotic and biotic factors, including breeders' selection. International and national projects have been organised in the field of preserving genetic resources of vegetables in the past, as well as nowadays in Serbia. Collected samples are stored in Plant Gene Bank of Serbia and in gene banks all over the world. Significant part is located in the collections of national institutes and faculties. Samples are described with passport data, while those in collections even in more detail. However, none of this is sufficient. In the last decades in Serbia, many populations and vegetable species have threatened to disappear. Many villages are disappearing, there are few growers of vegetables, and extinction of numerous valuable genotypes is accelerated by unfavourable weather conditions. Large number of cultivars and vegetable species that were

Nutrición vegetal (III).

OpenAIRE

Moyeja Santana, Juan de Jesús

2007-01-01

Editorial. Suniaga Q., José La codorniz fuente proteica para consumo humano. Díaz Cuellar, Doraida R. y González, Diomary Diagnóstico de parásitos gastrointestinales en bovinos y su importancia en la productividad de los sistemas ganaderos. Castillo O., Mayela; Hernández, Javier A.; Betancourt, Arquímedes y Suniaga Q., José Nutrición vegetal (II). Moyeja Santana, Juan de Jesús Nutrición vegetal (III). Moyeja Santana, Juan de Jesús Plagas chupadoras de las cítrica...

Nutrición vegetal (II).

OpenAIRE

Moyeja Santana, Juan de Jesús

2007-01-01

Editorial. Suniaga Q., José La codorniz fuente proteica para consumo humano. Díaz Cuellar, Doraida R. y González, Diomary Diagnóstico de parásitos gastrointestinales en bovinos y su importancia en la productividad de los sistemas ganaderos. Castillo O., Mayela; Hernández, Javier A.; Betancourt, Arquímedes y Suniaga Q., José Nutrición vegetal (II). Moyeja Santana, Juan de Jesús Nutrición vegetal (III). Moyeja Santana, Juan de Jesús Plagas chupadoras de las cítrica...

Genetically engineered plants with increased vegetative oil content

Science.gov (United States)

Benning, Christoph

2017-05-23

The invention relates to genetically modified agricultural plants with increased oil content in vegetative tissues, as well as to expression systems, plant cells, seeds and vegetative tissues related thereto.

Gelation and interfacial behaviour of vegetable proteins

NARCIS (Netherlands)

Vliet, T. van; Martin, A.H.; Bos, M.A.

2002-01-01

Recent studies on gelation and interfacial properties of vegetable protiens are reviewed. Attention is focused on legume proteins, mainly soy proteins, and on wheat proteins. The rheological properteis of vegetable protein gels as a function of heating time or temperature is discussed as well as the

Gelation and interfacial behaviour of vegetable proteins

NARCIS (Netherlands)

Vliet, van T.; Martin, A.H.; Bos, M.A.

2002-01-01

Recent studies on gelation and interfacial properties of vegetable proteins are reviewed. Attention is focused on legume proteins, mainly soy proteins, and on wheat proteins. The rheological properties of vegetable protein gels as a function of heating time or temperature is discussed as well as the

Technical efficiency of irrigated vegetable production among ...

African Journals Online (AJOL)

This study was carried out to analyse the technical efficiency of irrigated vegetable production among smallholder farmers in the guinea savannah, Nigeria, and determine the cost and returns on irrigated vegetable production. Two-stage sampling technique was used, purposive selection of two states and three Local ...

Spectrophotometric Determination of Nitrate in Vegetables Using ...

African Journals Online (AJOL)

DR. MIKE HORSFALL

ABSTRACT: A rapid and sensitive spectrophotometric method for the determination of nitrate in vegetables is described. The method is based on the measurement of the absorbance of yellow sodium nitrophenoxide formed via the reaction of phenol with the vegetable-based nitrate in presence of sulphuric acid.

Role of vegetation on river bank accretion

NARCIS (Netherlands)

Vargas Luna, A.

2016-01-01

There is rising awareness of the need to include the effects of vegetation in studies dealing with the morphological response of rivers. Vegetation growth on river banks and floodplains alters the river bed topography, reduces the bank erosion rates and enhances the development of new floodplains

Karoo biome: a preliminary synthesis. Part 2 - vegetation and history

CSIR Research Space (South Africa)

Cowling, RM

1987-01-01

Full Text Available and soil erosion. The focus of this volume is vegetation and its history. Included are chapters on vegetation physiognomy, plant growth, vegetation change, phytogeography, palaeo-ecology, palaeontology and archaeology...

Arsenic uptake and speciation in vegetables grown under greenhouse conditions.

Science.gov (United States)

Smith, E; Juhasz, A L; Weber, J

2009-04-01

The accumulation of arsenic (As) by vegetables is a potential human exposure pathway. The speciation of As in vegetables is an important consideration due to the varying toxicity of different As species. In this study, common Australian garden vegetables were hydroponically grown with As-contaminated irrigation water to determine the uptake and species of As present in vegetable tissue. The highest concentrations of total As were observed in the roots of all vegetables and declined in the aerial portions of the plants. Total As accumulation in the edible portions of the vegetables decreased in the order radish > mung bean > lettuce = chard. Arsenic was present in the roots of radish, chard, and lettuce as arsenate (As(V)) and comprised between 77 and 92% of the total As present, whereas in mung beans, arsenite (As(III)) comprised 90% of the total As present. In aerial portions of the vegetables, As was distributed equally between both As(V) and As(III) in radish and chard but was present mainly as As(V) in lettuce. The presence of elevated As in vegetable roots suggests that As species may be complexed by phytochelatins, which limits As translocation to aerial portions of the plant.

Bonneville - Hood River Vegetation Management Environmental Assessment

Energy Technology Data Exchange (ETDEWEB)

N/A

1998-08-01

To maintain the reliability of its electrical system, BPA, in cooperation with the U.S. Forest Service, needs to expand the range of vegetation management options used to clear unwanted vegetation on about 20 miles of BPA transmission line right-of-way between Bonneville Dam and Hood River; Oregon, within the Columbia Gorge National Scenic Area (NSA). We propose to continue controlling undesirable vegetation using a program of Integrated Vegetation Management (IVM) which includes manual, biological and chemical treatment methods. BPA has prepared an Environmental Assessment (EA) (DOE/EA-1257) evaluating the proposed project. Based on the analysis in the EA, BPA has determined that the proposed action is not a major Federal action significantly affecting the quality of the human environment, within the meaning of the National Environmental Policy Act (NEPA) of 1969. Therefore, the preparation of an Environmental Impact Statement (EIS) is not required and BPA is issuing this FONSI.

Broad-Scale Environmental Conditions Responsible for Post-Fire Vegetation Dynamics

Directory of Open Access Journals (Sweden)

Stuart E. Marsh

2010-11-01

Full Text Available Ecosystem response to disturbance is influenced by environmental conditions at a number of scales. Changes in climate have altered fire regimes across the western United States, and have also likely altered spatio-temporal patterns of post-fire vegetation regeneration. Fire occurrence data and a vegetation index (NDVI derived from the NOAA Advanced Very High Resolution Radiometer (AVHRR were used to monitor post-fire vegetation from 1989 to 2007. We first investigated differences in post-fire rates of vegetation regeneration between ecoregions. We then related precipitation, temperature, and elevation records at four temporal scales to rates of post-fire vegetation regeneration to ascertain the influence of climate on post-fire vegetation dynamics. We found that broad-scale climate factors are an important influence on post-fire vegetation regeneration. Most notably, higher rates of post-fire regeneration occurred with warmer minimum temperatures. Increases in precipitation also resulted in higher rates of post-fire vegetation growth. While explanatory power was slight, multiple statistical approaches provided evidence for real ecological drivers of post-fire regeneration that should be investigated further at finer scales. The sensitivity of post-disturbance vegetation dynamics to climatic drivers has important ramifications for the management of ecosystems under changing climatic conditions. Shifts in temperature and precipitation regimes are likely to result in changes in post-disturbance dynamics, which could represent important feedbacks into the global climate system.

Corrosividade causada por soluções produzidas com creosoto vegetal

Directory of Open Access Journals (Sweden)

Paes Juarez Benigno

2002-01-01

Full Text Available Esta pesquisa teve como objetivo avaliar a corrosividade de soluções preservativas preparadas com creosoto vegetal. Por destilação do alcatrão vegetal, obteve-se o creosoto vegetal bruto, recuperado à temperatura de 110-255 masculineC. Uma fração deste destilado foi lavada com solução a 9% de bicarbonato de sódio, obtendo-se o creosoto vegetal purificado. Ambas as frações foram enriquecidas com 3% de naftenato de cobre; 3% de naftenato de zinco; 3% de naftenato de cobalto; 2% de TBTO; 2% de tribromofenato de tributil-estanho; 2% de pentaclorofenol; ou 0,4% de trióxido de arsênico. Foram preparadas 16 soluções preservativas, sendo 14 enriquecidas, além do creosoto vegetal bruto e do creosoto vegetal purificado. Placas de aço SAE 1006 foram expostas por 6 horas às temperaturas de 25, 45 e 100 masculineC, à ação corrosiva dessas soluções. A corrosividade das soluções de creosoto vegetal foi comparada à corrosividade causada pelo creosoto mineral. As soluções preparadas com creosoto vegetal purificado foram menos corrosivas que suas similares preparadas com creosoto vegetal bruto, sem, no entanto, atingir a baixa corrosividade do creosoto mineral.

Vegetation (MCV / NVCS) Mapping Projects - California [ds515

Data.gov (United States)

California Natural Resource Agency — This metadata layer shows the footprint of vegetation mapping projects completed in California that have used the Manual California of Vegetation ( MCV 1st edition)...

Vegetation - Anza-Borrego Desert State Park [ds165

Data.gov (United States)

California Natural Resource Agency — The Anza Borrego Desert State Park (ABDSP) Vegetation Map depicts vegetation within the Park and its surrounding environment. The map was prepared by the Department...

Osmotic dehydration of fruits and vegetables: a review

OpenAIRE

Yadav, Ashok Kumar; Singh, Satya Vir

2012-01-01

The main cause of perishability of fruits and vegetables are their high water content. To increase the shelf life of these fruits and vegetables many methods or combination of methods had been tried. Osmotic dehydration is one of the best and suitable method to increase the shelf life of fruits and vegetables. This process is preferred over others due to their vitamin and minerals, color, flavor and taste retention property. In this review different methods, treatments, optimization and effec...

Measurement Of Technical Efficiency In Irrigated Vegetable ...

African Journals Online (AJOL)

This study measured technical efficiency and identified its determinants in irrigated vegetable production in Nasarawa State of Nigeria using a stochastic frontier model. A complete enumeration of 193 NADP-registered vegetable farmers was done. The predicted farm technical efficiency ranges from 25.94 to 96.24 per cent ...

Classification and mapping of rangeland vegetation physiognomic ...

African Journals Online (AJOL)

Plot vegetation species growth form, cover and height data were collected from 450 sampling sites based on eight spectral strata generated using unsupervised image classification. Field data were grouped at four levels of seven, six, three and two vegetation physiognomic classes which were subjected to both ML and ...

Weed Identification and Control in Vegetable Crops.

Science.gov (United States)

Ferretti, Peter A., Comp.

This agriculture extension service publication from Pennsylvania State University examines weed control and identification in vegetable crops. Contents include: (1) Types of weeds; (2) Reducing losses caused by weeds, general control methods and home garden weed control; (3) How herbicides are used; (4) Specific weeds in vegetable plantings; and…

Radiation processing of foods: fruits and vegetables

International Nuclear Information System (INIS)

Thomas, Paul

1990-01-01

Post-harvest irradiation of fruits and vegetables improves their shelf-life by: (1) delaying ripening and senescence of fruits, (2) controlling fungal diseases, (3) inhibiting sprouting, and (4) disinfestation. Nutritional and quality aspects of irradiated fruits and vegetables are discussed. Commercial prospects are briefly described. (M.G.B.)

Transmission System Vegetation Management Program. Draft Environmental Impact Statement

International Nuclear Information System (INIS)

1999-01-01

Bonneville is responsible for maintaining a network of 24,000 kilometers (km) or 15,000 miles (mi.) of electric transmission lines and 350 substations in a region of diverse vegetation. This vegetation can interfere with electric power flow, pose safety problems for Bonneville and the public, and interfere with their ability to maintain these facilities. They need to (1) keep vegetation away from the electric facilities; (2) increase their program efficiency and consistency; (3) review herbicide use (under increased public scrutiny); and (4) maximize the range of tools they can use while minimizing environmental impact (Integrated Vegetation Management). This DEIS establishes Planning Steps for managing vegetation for specific projects (to be tiered to this EIS). In addition to No Action (current practice), alternatives are presented for Rights-of-way, Electric Yards, and Non-electric Facilities (landscaping, work yards). Four vegetation control methods are analyzed: manual, mechanical, herbicide, and biological. Also evaluated are 24 herbicide active ingredients and 4 herbicide application techniques (spot, localized, broadcast, and aerial). For rights-of-way, they consider three sets of alternatives: alternative management approaches (time-driven or establishing low-growing plant communities); alternative method packages; and, if herbicides are in a methods package, alternative vegetation selections (noxious weeds, deciduous, or any vegetation). For electric yards, one herbicide-use alternative is considered. For non-electric facilities, two method package alternatives are considered. For rights-of-way, the environmentally preferred alternative(s) would use manual, mechanical, and biological control methods, as well as spot and localized herbicide applications for noxious and deciduous plant species; the BPA-preferred alternative(s) would add broadcast and aerial herbicide applications, and would use herbicides on any vegetation. Both would favor a management

Vegetation Parameter Extraction Using Dual Baseline Polarimetric SAR Interferometry Data

Science.gov (United States)

Zhang, H.; Wang, C.; Chen, X.; Tang, Y.

2009-04-01

For vegetation parameter inversion, the single baseline polarimetric SAR interferometry (POLinSAR) technique, such as the three-stage method and the ESPRIT algorithm, is limited by the observed data with the minimum ground to volume amplitude ration, which effects the estimation of the effective phase center for the vegetation canopy or the surface, and thus results in the underestimated vegetation height. In order to remove this effect of the single baseline inversion techniques in some extend, another baseline POLinSAR data is added on vegetation parameter estimation in this paper, and a dual baseline POLinSAR technique for the extraction of the vegetation parameter is investigated and improved to reduce the dynamic bias for the vegetation parameter estimation. Finally, the simulated data and real data are used to validate this dual baseline technique.

Advances in Studies on Natural Preservativesfor Fruits and Vegetables

Science.gov (United States)

Gao, Haisheng; Shi, Pengbao; Zhao, Yuhua

The author introduced g eneral research and application situations of natural preservatives for fruits and vegetables all over the world these years, and summarized application of vegetation of Murraya in Rutaceae, Cinnamomum in Lauraceae, Artemisia in Compositae and other families and genera on fruits and vegetables preservation and fresh-keeping. Decoction or extraction of Chinese traditional medicine, such as Alpinia Officinarum, Amarphalus Konjac K., stemona etc, could be used in fresh-keeping for orange, apple, strawberry, edible fungi and so on. Garlic could be used in fresh-keeping for orange. Phytic acid and fresh-keeping agents compounded with Phytic acid could extend storage periods of easily rotting fruits and vegetables, such as strawberry, banana, cantaloup, edible fungi and so on, and better keep original fresh condition. Extraction of Snow Fresh, Semper Fresh, Arthropod shell extraction, and halite also had better effect on preservation and fresh-keeping for fruits and vegetables. Main problems exsited in the application of natural preservatives for fruits and vegetables were showed in this article and the applying prospect were discussed too.

Application of a Coupled Vegetation Competition and Groundwater Simulation Model to Study Effects of Sea Level Rise and Storm Surges on Coastal Vegetation

Directory of Open Access Journals (Sweden)

Su Yean Teh

2015-09-01

Full Text Available Global climate change poses challenges to areas such as low-lying coastal zones, where sea level rise (SLR and storm-surge overwash events can have long-term effects on vegetation and on soil and groundwater salinities, posing risks of habitat loss critical to native species. An early warning system is urgently needed to predict and prepare for the consequences of these climate-related impacts on both the short-term dynamics of salinity in the soil and groundwater and the long-term effects on vegetation. For this purpose, the U.S. Geological Survey’s spatially explicit model of vegetation community dynamics along coastal salinity gradients (MANHAM is integrated into the USGS groundwater model (SUTRA to create a coupled hydrology–salinity–vegetation model, MANTRA. In MANTRA, the uptake of water by plants is modeled as a fluid mass sink term. Groundwater salinity, water saturation and vegetation biomass determine the water available for plant transpiration. Formulations and assumptions used in the coupled model are presented. MANTRA is calibrated with salinity data and vegetation pattern for a coastal area of Florida Everglades vulnerable to storm surges. A possible regime shift at that site is investigated by simulating the vegetation responses to climate variability and disturbances, including SLR and storm surges based on empirical information.

Study of microbiological background of herbal ingredients and dairy-vegetable compositions

Directory of Open Access Journals (Sweden)

D. V. Kharitonov

2016-01-01

Full Text Available The rates of microbiological safety of powdery vegetables, vegetable-milk compositions, compound desserts have been studied. No pathogenic germs (incl. salmonella, Escherichia coli, yeast, nonspore-forming bacteria B cereus have been detected in powdery vegetable samples. The number of mesophilic aerobic and facultative anaerobic microorganisms as well as amount of molds does not exceed safety index normalized by the legislation. Proteolytic microorganisms compose the basic microflora of powdery vegetables. Microbiological background of vegetable and milk basis is characterized by the presence of microorganisms differed by different resistance to the medium conditions – рН value, presence of oxygen and high temperatures impact. Enrichment of milk base by vegetable components necessitates to adjust the thermal effect regimes prescribed for milk treatment without additional ingredients. Introduction of vegetable ingredients into milk base is accompanied by polysemantic effect of high temperatures on microorganisms of polycomponent milk – vegetable base. On the one hand introduction of vegetable raw material into milk enhances inhibitory temperature effect on microbial cells due to transition of the medium рН into sour side; on the other hand presence of vegetable raw material particles protects microorganisms against sensitive effect of high temperature. Microflora of vegetable-milk compositions after heat treatment as well as ready-made desserts on their base was presented by spore-forming bacillus the number of which is correlated by their number in the initial raw material. In order to choose the optimal regime of heat treatment all processes running during heat treatment and particularly microbiological and physical-chemical degradation of polysaccharides of vegetables cell structures.

Will European agricultural policy for school fruit and vegetables improve public health? A review of school fruit and vegetable programmes.

Science.gov (United States)

de Sa, Joia; Lock, Karen

2008-12-01

For the first time, public health, particularly obesity, is being seen as a driver of EU agricultural policy. In 2007, European Ministers of Agriculture were asked to back new proposals for school fruit and vegetable programmes as part of agricultural reforms. In 2008, the European Commission conducted an impact assessment to assess the potential impact of this new proposal on health, agricultural markets, social equality and regional cohesion. A systematic review of the effectiveness of interventions to promote fruit and/or vegetable consumption in children in schools, to inform the EC policy development process. School schemes are effective at increasing both intake and knowledge. Of the 30 studies included, 70% increased fruits and vegetables (FV) intake, with none decreasing intake. Twenty-three studies had follow-up periods >1 year and provide some evidence that FV schemes can have long-term impacts on consumption. Only one study led to both increased fruit and vegetable intake and reduction in weight. One study showed that school fruit and vegetable schemes can also help to reduce inequalities in diet. Effective school programmes have used a range of approaches and been organized in ways which vary nationally depending on differences in food supply chain and education systems. EU agriculture policy for school fruits and vegetables schemes should be an effective approach with both public health and agricultural benefits. Aiming to increase FV intake amongst a new generation of consumers, it will support a range of EU policies including obesity and health inequalities.

Substitution or addition? How overweight and obese adults incorporate vegetables into their diet during a randomized controlled vegetable feeding trial

Science.gov (United States)

Objective: When attempting to eat healthier, individuals may add vegetables to their diet (addition) without changing other eating behaviors. Alternatively, individuals adding vegetables may decrease consumption of other foods (substitution). Distinguishing between the two means of incorporation of ...

Waste vegetable oil survey report

Energy Technology Data Exchange (ETDEWEB)

MacLeod, R. [Science enterprise Algoma seA, Sault Ste. Marie, ON (Canada)

2009-02-06

This study was conducted to estimate potential sources of feedstock waste oils for biodiesel production in the Sault Ste. Marie region of Ontario. Two feedstocks were investigated over a period of several months, notably cooking oil and waste vegetable oil. The study was conducted to examine oil throughput, collection practices, and to gauge interest in local initiatives. A distribution list of commercial restaurant listings was developed, and surveys were conducted with members of private enterprises, city government, and non-profit stakeholders in the region. Average volumes of waste vegetable oil were presented for different types of restaurants. The various types of oil used in the restaurants were also quantified. Results of the study showed a positive public response to the idea of a local biodiesel initiative. Steak house, fast food, and Italian establishments generated the largest portion of waste vegetable oil amongst survey respondents. However, the highest response rates came from establishments with little or no oil consumption. Many franchise fast food restaurants are already in contracts with waste oil removal companies. 3 tabs., 3 figs.

THEORETICAL MODELLING STUDY ON THE RELATIONSHIP BETWEEN MULTI-FREQUENCY MICROWAVE VEGETATION INDEX AND VEGETATION PROPERTIES (OPTICAL DEPTH AND SINGLE SCATTERING ALBEDO

Directory of Open Access Journals (Sweden)

S. Talebi

2018-04-01

Full Text Available This paper presents a theoretical study of derivation Microwave Vegetation Indices (MVIs in different pairs of frequencies using two methods. In the first method calculating MVI in different frequencies based on Matrix Doubling Model (to take in to account multi scattering effects has been done and analyzed in various soil properties. The second method was based on MVI theoretical basis and its independency to underlying soil surface signals. Comparing the results from two methods with vegetation properties (single scattering albedo and optical depth indicated partial correlation between MVI from first method and optical depth, and full correlation between MVI from second method and vegetation properties. The second method to derive MVI can be used widely in global microwave vegetation monitoring.

Effects of sand fences on coastal dune vegetation distribution

Science.gov (United States)

Grafals-Soto, Rosana

2012-04-01

Sand fences are important human adjustments modifying the morphology of developed shores. The effects of sand fences on sediment transport and deposition in their initial stages have been well studied, but little is known about the effect of deteriorated sand fences that have become partially buried low scale barriers within the dune, potentially benefiting vegetation growth by protecting it from onshore stress. Data on vegetation, topography and fence characteristics were gathered at three dune sites in Ocean City, New Jersey on September 2007 and March 2008 to evaluate the effect of fences within the dune on vegetation distribution. Variables include: distance landward of dune toe, degree of sheltering from onshore stressors, net change in surface elevation (deposition or erosion), vegetation diversity and density, presence of remnant fence, and distance landward of fence. Results for the studied environment reveal that 1) vegetation diversity or density does not increase near remnant fences because most remnants are lower than average vegetation height and can not provide shelter; but 2) vegetation distribution is related to topographic variables, such as degree of sheltering, that are most likely the result of sand accretion caused by fence deployment. Fence deployment that prioritizes the creation of topographically diverse dunes within a restricted space may increase the diversity and density of the vegetation, and the resilience and value of developed dunes. Managers should consider the benefits of using sand fences on appropriately wide beaches to create a protective dune that is also diverse, functional and better able to adapt to change.

Whole grain gluten-free vegetable spicy snacks

Science.gov (United States)

Four kinds of spicy snacks (gluten-free, whole grains with fresh vegetables, low in fat, sugar and salt) were evaluated. Acceptance of spicy snacks tested were Carrot-Garlic 77%, Broccoli-Garlic 68%, Spinach-Garlic 61% and Red Onion 53%. This is the first report of spicy gluten-free, 50% vegetable...