WorldWideScience

Sample records for variegated epipremnum aureum

  1. Analysis of plant function as bio-thermal-conditioner using Pothos (Epipremnum aureum)

    Energy Technology Data Exchange (ETDEWEB)

    Nakazato, Tadashi, E-mail: nack-art@y7.dion.ne.jp [Nakadai Junior High School, 1-56-23 Nakadai Itabashi-ku, Tokyo 174-0064 (Japan); Inagaki, Terumi, E-mail: hotaru@mx.ibaraki.ac.jp [Department of Mechanical Engineering, Ibaraki University, 4 Chome, Nakanarusawa 12-1, Hitachi, Ibaraki 316-8511 (Japan)

    2012-03-20

    Plants absorb carbon dioxide by photosynthesis using solar energy and use thermal energy in the atmosphere by transpiration. Paying attention to the excellent functions that plants perform, we have measured the daily variation of the temperature and humidity on plant circumference of an individual level using Pothos (Epipremnum aureum), and have investigated the thermal conditioning effect of the plant irradiated white LED in a closed space covered with thermal insulation at the incubator adjusted temperature. The air-cooling effect of Pothos in a closed space at the incubator adjusted 23 Degree-Sign C was about 1 Degree-Sign C. High correlation between air temperature and that of soil was shown in the air-cooling effect, and long-time white light irradiation increased the air-cooling effect.

  2. Analysis of plant function as bio-thermal-conditioner using Pothos (Epipremnum aureum)

    International Nuclear Information System (INIS)

    Nakazato, Tadashi; Inagaki, Terumi

    2012-01-01

    Plants absorb carbon dioxide by photosynthesis using solar energy and use thermal energy in the atmosphere by transpiration. Paying attention to the excellent functions that plants perform, we have measured the daily variation of the temperature and humidity on plant circumference of an individual level using Pothos (Epipremnum aureum), and have investigated the thermal conditioning effect of the plant irradiated white LED in a closed space covered with thermal insulation at the incubator adjusted temperature. The air-cooling effect of Pothos in a closed space at the incubator adjusted 23 °C was about 1 °C. High correlation between air temperature and that of soil was shown in the air-cooling effect, and long-time white light irradiation increased the air-cooling effect.

  3. Epipremnum aureum and Dracaena braunii as indoor plants for enhanced bio-electricity generation in a plant microbial fuel cell with electrochemically modified carbon fiber brush anode.

    Science.gov (United States)

    Sarma, Pranab Jyoti; Mohanty, Kaustubha

    2018-04-13

    In this study, two different unexploited indoor plants, Epipremnum aureum and Dracaena braunii were used to produce clean and sustainable bio-electricity in a plant microbial fuel cell (PMFC). Acid modified carbon fiber brush electrodes as well as bare electrodes were used in both the PMFCs. A bentonite based clay membrane was successfully integrated in the PMFCs. Maximum performance of E. aureum was 620 mV which was 188 mV higher potential than D. braunii. The bio-electricity generation using modified electrode was 154 mV higher than the bare carbon fiber, probably due to the effective bacterial attachment to the carbon fiber owing to hydrogen bonding. Maximum power output of 15.38 mW/m 2 was obtained by E. aureum with an internal resistance of 200 Ω. Higher biomass yield was also obtained in case of E. aureum during 60 days of experiment, which may correlate with the higher bio-electricity generation than D. braunii. Copyright © 2018 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

  4. An experimental evaluation of adaptogenic potential of standardized epipremnum aureum leaf extract

    Directory of Open Access Journals (Sweden)

    Sreemoy Kanti Das

    2017-01-01

    Full Text Available Background: Stress is a normal part of everyday life but chronic stress can lead to a variety of stress-related illnesses including hypertension, anxiety, and depression. In the present investigation, standardized leaf extract of Epipremnumaureum was evaluated for its anti-stress potential. Materials and Methods: For the evaluation of anti-stress activity, groups of mice (n = 6 were subjected to forced swim stress and anoxic stress tolerance test in mice 1h after daily treatment of E.aureumextract. Diazepam (5 mg/kg was taken as a reference standard. Urinary vanillylmandelic acid (VMA and ascorbic acid were selected as noninvasive biomarkers to assess the anti-stress activity and plasma cortisol, blood ascorbic acid, and weight of adrenal were measured. The 24 h urinary excretion of VMA and ascorbic acid were determined by spectrophotometric methods in all groups under normal and stressed conditions. The hematological parameters (neutrophils, lymphocytes, and eosinophils were also determined. Results: Administration of E.aureumat doses of 400 and 600 mg/kg wasfound to be effective in inhibiting the stress induced urinary biochemical changes in a dose-dependent manner. Treatment with E. aureum extract prevents the rise in blood ascorbic acid and plasma cortisol. Moreover, the extract prevented the increase in weight of adrenal gland also significantly increased the anoxia stress tolerance time. Dose-dependent significant reduction in white blood cell count was observed in anoxic stress tolerance test as compared to stressed group. Conclusion: Hence, the present study provides scientific support for the positiveadaptogenic effect of E. aureum extract.

  5. Aureum Chaos

    Science.gov (United States)

    2003-01-01

    [figure removed for brevity, see original site] Released 11 November 2003Aureum Chaos is a large crater that was filled with sediment after its formation. After the infilling of sediment, something occurred that caused the sediment to be broken up into large, slumped blocks and smaller knobs. Currently, it is believed that the blocks and knobs form when material is removed from the subsurface, creating void space. Subsurface ice was probably heated, and the water burst out to the surface, maybe forming a temporary lake. Other areas of chaos terrain have large outflow channels that emanate from them, indicating that a tremendous amount of water was released.Image information: VIS instrument. Latitude -3.2, Longitude 331 East (29 West). 19 meter/pixel resolution.Note: this THEMIS visual image has not been radiometrically nor geometrically calibrated for this preliminary release. An empirical correction has been performed to remove instrumental effects. A linear shift has been applied in the cross-track and down-track direction to approximate spacecraft and planetary motion. Fully calibrated and geometrically projected images will be released through the Planetary Data System in accordance with Project policies at a later time. NASA's Jet Propulsion Laboratory manages the 2001 Mars Odyssey mission for NASA's Office of Space Science, Washington, D.C. The Thermal Emission Imaging System (THEMIS) was developed by Arizona State University, Tempe, in collaboration with Raytheon Santa Barbara Remote Sensing. The THEMIS investigation is led by Dr. Philip Christensen at Arizona State University. Lockheed Martin Astronautics, Denver, is the prime contractor for the Odyssey project, and developed and built the orbiter. Mission operations are conducted jointly from Lockheed Martin and from JPL, a division of the California Institute of Technology in Pasadena.

  6. Gouden ribzaad (Chaerophyllum aureum L.); onopgemerkt ingeburgerd

    NARCIS (Netherlands)

    Beringen, Ruud; Dirkse, Gerard M.; Moorsel, van René C.M.J.

    2005-01-01

    In 2005 is Gouden ribzaad (Chaerophyllum aureum) op drie verschillende locaties (in vier km-hokken) binnen Nederland vastgesteld. Gouden ribzaad is op twee plaatsen waarschijnlijk al 20 jaar aanwezig. Door de gelijkenis met Fluitenkruid (Anthriscus sylvestris), Knolribzaad (C. bulbosum) en Dolle

  7. Competing Hegemonic Projects within China's Variegated Capitalism

    DEFF Research Database (Denmark)

    Mulvad, Andreas Christian Møller

    2014-01-01

    This article investigates the variegated model of capitalism emerging in China through a comparative analysis of two contrasting local government approaches developed in Chongqing and Guangdong. From 2007 to 2012, elite politicians and intellectuals articulated the Chongqing and Guangdong models...... of his Guangdong counterpart Wang Yang. Future studies should expand upon the key theoretical insight of the paper: that the emergent variegated capitalism approach to political–economic analysis needs a stronger ideational component....

  8. Investigations on gamma ray induced chlorophyll variegated mutants

    International Nuclear Information System (INIS)

    Datta, S.K.; Dwivedi, A.K.; Banerji, B.K.

    1995-01-01

    Considering economic importance of chlorophyll variegation in floriculture trade an attempt was made for cytological, anatomical and biochemical analysis of four Bougainvillea and Lantana depressa chlorophyll variegated mutants for better and clear understanding of origin of chlorophyll variegation. No cytological evidence could be detected for their origin. Anatomical and biochemical examinations revealed that chlorophyll variegation in these mutants were due to changes in biosynthesis pathways and time of chlorophyll synthesis in palisade and spongy mesophyll cells. (author). 7 refs., 3 figs., 3 tabs

  9. Development and characterization of 27 microsatellite markers for the mangrove fern, Acrostichum aureum (Pteridaceae).

    Science.gov (United States)

    Yamamoto, Takashi; Tsuda, Yoshiaki; Mori, Gustavo Maruyama; Cruz, Mariana Vargas; Shinmura, Yoshimi; Wee, Alison K S; Takayama, Koji; Asakawa, Takeshi; Yamakawa, Takeru; Suleiman, Monica; Núñez-Farfán, Juan; Webb, Edward L; Watano, Yasuyuki; Kajita, Tadashi

    2016-09-01

    Twenty-seven nuclear microsatellite markers were developed for the mangrove fern, Acrostichum aureum (Pteridaceae), to investigate the genetic structure and demographic history of the only pantropical mangrove plant. Fifty-six A. aureum individuals from three populations were sampled and genotyped to characterize the 27 loci. The number of alleles and expected heterozygosity ranged from one to 15 and 0.000 to 0.893, respectively. Across the 26 polymorphic loci, the Malaysian population showed much higher levels of polymorphism compared to the other two populations in Guam and Brazil. Cross-amplification tests in the other two species from the genus determined that seven and six loci were amplifiable in A. danaeifolium and A. speciosum, respectively. The 26 polymorphic microsatellite markers will be useful for future studies investigating the genetic structure and demographic history of of A. aureum, which has the widest distributional range of all mangrove plants.

  10. Genetics Home Reference: mosaic variegated aneuploidy syndrome

    Science.gov (United States)

    ... In MVA syndrome, growth before birth is slow (intrauterine growth restriction). After birth, affected individuals continue to grow at ... InfoSearch: Warburton Anyane Yeboa syndrome KidsHealth from Nemours: Intrauterine Growth Restriction ... mosaic variegated aneuploidy syndrome 1 MalaCards: ...

  11. Rhododendron aureum Georgi formed a special soil microbial community and competed with above-ground plants on the tundra of the Changbai Mountain, China.

    Science.gov (United States)

    Wang, Xiaolong; Li, Lin; Zhao, Wei; Zhao, Jiaxin; Chen, Xia

    2017-09-01

    Rhododendron aureum Georgi is a perennial evergreen dwarf shrub that grows at all elevations within the alpine tundra of northern China. Previous research has investigated the plant communities of R. aureum ; however, little information is available regarding interspecific competition and underground soil microbial community composition. The objective of our study was to determine whether the presence of R. aureum creates a unique soil microbiome and to investigate the relationship between R. aureum and other plant species. Our study site ranged from 1,800 to 2,600 m above sea level on the northern slope of the Changbai Mountain. The results show that the soil from sites with an R. aureum community had a higher abundance of nitrogen-fixing bacteria and a higher resistance to pathogens than soils from sites without R. aureum . We emphasize that R. aureum promotes a unique soil microbial community structure that is distinct from those associated with other plants. Elevation and microbial biomass were the main influencing factors for plant community structure. Analysis of interspecific relationships reveals that R. aureum is negatively associated with most other dominant shrubs and herbs, suggesting interspecific competition. It is necessary to focus on other dominant species if protection and restoration of the R. aureum competition is to occur. In the future, more is needed to prove whether R. aureum decreases species diversity in the tundra ecosystems of Changbai Mountain.

  12. Population ecology of variegate darter (Etheostoma variatum) in Virginia

    Science.gov (United States)

    Argentina, Jane E.; Angermeier, Paul; Hallerman, Eric M.

    2013-01-01

    Variegate darters (Etheostoma variatum) were listed as endangered in Virginia in 1992. Reasons for listing included habitat degradation and concerns about current and future impacts of coal mining throughout their Virginia range. Prior to this research, little was known about variegate darter distribution, habitat use, or populations in Virginia. Two primary goals of this research were to gain knowledge about the current population ecology and the relationship between landscape-level factors (e.g., land cover changes, watershed size, isolation from other populations) on current and past variegate darter population sizes.We investigated distribution, habitat suitability, population genetics, and population size and structure of variegate darters in the upper Big Sandy River drainage, Buchanan, Dickenson, and Wise Co., Virginia. Our results indicate variegate darters are primarily found in the Levisa Fork, with highest densities and abundances between its confluence with Dismal Creek and the Virginia-Kentucky border. Sporadic occurrences in smaller tributaries to the Levisa and Tug forks indicate they exist more widely in low densities, especially near the confluence with the Tug and Levisa mainstems. Detection of variegate darters in smaller tributaries was inconsistent, with reach-level occupancy estimates varying among years. We detected young-of-year variegate darters every year we sampled, but age 1+ darters were indistinguishable from older darters based on standard length.Variegate darter population size and stability in Virginia were estimated via multiple methods, including site occupancy surveys, mark-recapture studies, and population genetic analysis. Using mark-recapture methods at five sites, we estimated overall population size in 2011 to be approximately 12,800 individuals in the 35-km reach between the Levisa Fork - Dismal Creek confluence and the Virginia-Kentucky border. Age structure seemed stable, with breeding adults and young-of-year collected

  13. Immediate skin and mucosal symptoms from pot plants and vegetables in gardeners and greenhouse workers

    DEFF Research Database (Denmark)

    Paulsen, E; Skov, Per Stahl; Andersen, Klaus Ejner

    1998-01-01

    cacti, Stephanotis floribunda, Euphorbia pulcherrima and Gerbera reactions. Other new species implicated in immediate-type reactions included Ficus pumila, Gardenia jasminoides, Hibiscus rosa-sinensis, Campanula, Columnea, Epipremnum aureum, Pelargonium and Primula vulgaris. Because of the high...

  14. The high frequency of variegated forms after in vitro mutagenesis in Saintpaulia ionantha Wendl.

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    Marek Gaj

    2014-01-01

    Full Text Available The leaf-explants of Saintpaulia ionantha Wendl. var.'miniature' were treated by different doses of MNH and cultured on shoot regeneration medium. A strong toxic effect of some MNH doses on explant survival during the first two subcultures was noticed. The explants surviving treatment regenerated shoots with the efficiency comparable to the control. The high number of shoots regenerated from mutagenised leaves showed chlorophyll chimerism (so-called variegated forms. The use of 5 mM MNH for 1.5 or 2 h was found very effective, as 100% of survived explants regenerated variegated shoots. Besides hundreds of variegated forms also leaf-shape and flower-colour variants were observed in MNH-treated culture. Somaclonal variation was not observed in the control culture. The results indicate the great efficiency of in vitro applied MNH for in-duction of morphological variants of Saintpaulia, and especially variegated forms.

  15. Molecular Basis Underlying Leaf Variegation of a Moth Orchid Mutant (Phalaenopsis aphrodite subsp. formosana

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    Chi-Chu Tsai

    2017-07-01

    Full Text Available Leaf variegation is often the focus of plant breeding. Here, we studied a variegated mutant of Phalaenopsis aphrodite subsp. formosana, which is usually used as a parent of horticultural breeding, to understand its anatomic and genetic regulatory mechanisms in variegation. Chloroplasts with well-organized thylakoids and starch grains were found only in the mesophyll cells of green sectors but not of yellow sectors, confirming that the variegation belongs to the chlorophyll type. The two-dimensional electrophoresis and LC/MS/MS also reveal differential expressions of PsbP and PsbO between the green and yellow leaf sectors. Full-length cDNA sequencing revealed that mutant transcripts were caused by intron retention. When conditioning on the total RNA expression, we found that the functional transcript of PsbO and mutant transcript of PsbP are higher expressed in the yellow sector than in the green sector, suggesting that the post-transcriptional regulation of PsbO and PsbP differentiates the performance between green and yellow sectors. Because PsbP plays an important role in the stability of thylakoid folding, we suggest that the negative regulation of PsbP may inhibit thylakoid development in the yellow sectors. This causes chlorophyll deficiency in the yellow sectors and results in leaf variegation. We also provide evidence of the link of virus CymMV and the formation of variegation according to the differential expression of CymMV between green and yellow sectors.

  16. Early Jurassic diversification of pycnodontiform fishes (Actinopterygii, Neopterygii) after the end-Triassic extinction event: evidence from a new genus and species, Grimmenodon aureum.

    Science.gov (United States)

    Stumpf, Sebastian; Ansorge, Jörg; Pfaff, Cathrin; Kriwet, Jürgen

    2017-07-04

    A new genus and species of pycnodontiform fishes, Grimmenodon aureum , from marginal marine, marine-brackish lower Toarcian ( Harpoceras exaratum ammonite subzone) clay deposits of Grimmen in northeastern Germany is described. The single specimen represents a diagnostic left prearticular dentition characterized by unique tooth arrangement and ornamentation patterns. Grimmenodon aureum , gen. et sp. nov., is the second unambiguously identified pycnodontiform species from the Early Jurassic, in addition to Eomesodon liassicus from the early Lower Jurassic of western Europe. We also report an indeterminate pycnodontiform tooth crown from the upper Pliensbachian ( Pleuroceras apyrenum ammonite subzone) of the same site. The material expands the Early Jurassic range of pycnodontiforms significantly northwards and confirms their presence before and immediately following the onset of the Toarcian Oceanic Anoxic Event (T-OAE) in the marginal marine ecosystems south of the Fennoscandian Shield. Moreover, the new records indicate that the Early Jurassic diversity of pycnodontiform fishes was greater than previously assumed and probably equaled that of the Late Triassic. Therefore, it is hypothesized that the Triassic-Jurassic mass extinction event did not affect pycnodontiform fishes significantly. Micro-computed tomography was used to study the internal anatomy of the prearticular of Grimmenodon aureum , gen. et sp. nov. Our results show that no replacement teeth were formed within the tooth-bearing bone but rather were added posteriorly to functional teeth. http://zoobank.org/urn:lsid:zoobank.org:pub:A56BDE9C-40C4-4CFA-9C2E-F5FA35A66F2 Citation for this article: Stumpf, S., J. Ansorge, C. Pfaff, and J. Kriwet. 2017. Early Jurassic diversification of pycnodontiform fishes (Actinopterygii, Neopterygii) after the end-Triassic extinction event: Evidence from a new genus and species, Grimmenodon aureum . Journal of Vertebrate Paleontology. DOI: 10.1080/02724634.2017.1344679.

  17. Characterization of variegate porphyria mutations using a minigene approach.

    Science.gov (United States)

    Granata, Barbara Xoana; Baralle, Marco; De Conti, Laura; Parera, Victoria; Rossetti, Maria Victoria

    2015-01-01

    Porphyrias are a group of metabolic diseases that affect the skin and/or nervous system. In 2008, three unrelated patients were diagnosed with variegate porphyria at the CIPYP (Centro de Investigaciones sobre Porfirinas y Porfirias). Sequencing of the protoporphyrinogen oxidase gene, the gene altered in this type of porphyria, revealed three previously undescribed mutations: c.338+3insT, c.807G>A, and c.808-1G>C. As these mutations do not affect the protein sequence, we hypothesized that they might be splicing mutations. RT-PCRs performed on the patient's mRNAs showed normal mRNA or no amplification at all. This result indicated that the aberrant spliced transcript is possibly being degraded. In order to establish whether they were responsible or not for the patient's disease by causing aberrant splicing, we utilized a minigene approach. We found that the three mutations lead to exon skipping; therefore, the abnormal mRNAs are most likely degraded by a mechanism such as nonsense-mediated decay. In conclusion, these mutations are responsible for the disease because they alter the normal splicing pathway, thus providing a functional explanation for the appearance of disease and highlighting the use of minigene assays to complement transcript analysis.

  18. The microbial flora of the different gut regions of the variegated ...

    African Journals Online (AJOL)

    The microbial flora of the gut regions and gut contents of the variegated grasshopper Zonocerus variegatus instars was studied using the pour plate technique. The gut sections (Fore-, mid-, and hind-gut) harboured a variety organisms mainly bacteria, fungi and mould. Yeasts species isolated were Candida, ...

  19. A Study on the Feeding and Growth Patterns of the Variegated ...

    African Journals Online (AJOL)

    The feeding and growth patterns of the variegated grasshopper, Zonocerus variegatus (L) were studied in the laboratory to ascertain the amount of food intake, food assimilated and faeces excreted by its nymph and adult stages on a mixed diet of Cassava (Manihot esculenta) and Siam weed (Chromolaena odorata) leaves.

  20. DNA triplet repeats mediate heterochromatin-protein-1-sensitive variegated gene silencing.

    Science.gov (United States)

    Saveliev, Alexander; Everett, Christopher; Sharpe, Tammy; Webster, Zoë; Festenstein, Richard

    2003-04-24

    Gene repression is crucial to the maintenance of differentiated cell types in multicellular organisms, whereas aberrant silencing can lead to disease. The organization of DNA into chromatin and heterochromatin is implicated in gene silencing. In chromatin, DNA wraps around histones, creating nucleosomes. Further condensation of chromatin, associated with large blocks of repetitive DNA sequences, is known as heterochromatin. Position effect variegation (PEV) occurs when a gene is located abnormally close to heterochromatin, silencing the affected gene in a proportion of cells. Here we show that the relatively short triplet-repeat expansions found in myotonic dystrophy and Friedreich's ataxia confer variegation of expression on a linked transgene in mice. Silencing was correlated with a decrease in promoter accessibility and was enhanced by the classical PEV modifier heterochromatin protein 1 (HP1). Notably, triplet-repeat-associated variegation was not restricted to classical heterochromatic regions but occurred irrespective of chromosomal location. Because the phenomenon described here shares important features with PEV, the mechanisms underlying heterochromatin-mediated silencing might have a role in gene regulation at many sites throughout the mammalian genome and modulate the extent of gene silencing and hence severity in several triplet-repeat diseases.

  1. Genética de Coffea. XVIII - Variegação no cafeeiro Leaf variegation in coffee plants

    Directory of Open Access Journals (Sweden)

    A. Carvalho

    1954-01-01

    Full Text Available A variegação das fôlhas do cafeeiro tanto em plantas novas como em plantas adultas tem sido freqüentemente observada. Plantas variegadas podem ocorrer espontaneamente nas progénies das diversas variedades de Coffea arabica L., motivo porque não devem constituir variedade distinta dessa espécie, como foi proposto por Cramer. As plantas variegadas em estudo puderam ser agrupadas em duas categorias, isto é, aquelas em que a variegação não se acha associada com anomalias na forma e textura das folhas e aquelas nas quais a variegação é acompanhada dessas anomalias. No primeiro grupo o padrão de variegação não é uniforme para tôdas as fôlhas, enquanto no segundo grupo o padrão é semelhante para tôda a planta. Usualmente apenas alguns ramos das plantas do primeiro grupo são variegados. Observou-se algumas vezes que as duas fôlhas do mesmo verticilo têm padrões semelhantes de variegação. Encontraram-se também fôlhas variegadas nas quais a metade da lâmina é variegada ou mesmo albina, enquanto a outra metade apresenta a côr verde normal. Os dados genéticos obtidos, embora preliminares para a maioria das plantas variegadas em investigação, permitiram concluir que o padrão de variegação da planta 180, pertencente ao primeiro grupo, é herdado pelo citoplasma e não é transmitido pelo pólen. O padrão de variegação da planta 253-21, pertencente ao segundo grupo, no entanto, parece ser transmitido pelo polen.Leaf variegation in coffee plants is a relatively common abnormality, and variegated seedlings have been found to occur spontaneously in progenies of most varieties of Coffea arabica L. For this reason it is thought that variegated types should not be described as distinct varieties, as it was done before by Cramer. Variegated coffee plants can be grouped in two categories : first, those in which variegated leaves are normal in shape and texture, but do not show a uniform variegation pattern; and second, those

  2. Obervations on the Induction of Position Effect Variegation of Euchromatic Genes in Drosophila Melanogaster

    OpenAIRE

    Pokholkova, G. V.; Makunin, I. V.; Belyaeva, E. S.; Zhimulev, I. F.

    1993-01-01

    In the T(1;2)dor(var7) translocation, the 1A-2B7-8 segment of the X chromosome is brought to the vicinity of 2R-chromosome heterochromatin resulting in position effect variegation of dor, BR-C and more distal genes, as well as compaction of chromatin in this segment. By irradiation of T(1;2)dor(var7), nine reversions (rev) to a normal phenotype were recovered. In two cases (rev27, rev226), the 1A-2B7-8 section is relocated to the 19A region of the X chromosome, forming free duplications (1A-2...

  3. Arabidopsis VARIEGATED 3 encodes a chloroplasttargeted, zinc-finger protein required for chloroplast and palisade cell development

    DEFF Research Database (Denmark)

    Næsted, Henrik; Holm, A.; Jenkins, T.

    2004-01-01

    The stable, recessive Arabidopsis variegated 3 (var3) mutant exhibits a variegated phenotype due to somatic areas lacking or containing developmentally retarded chloroplasts and greatly reduced numbers of palisade cells. The VAR3 gene, isolated by transposon tagging, encodes the 85.9 kDa VAR3...... that pigment profiles are qualitatively similar in wild type and var3, although var3 accumulates lower levels of chlorophylls and carotenoids. These results indicate that VAR3 is a part of a protein complex required for normal chloroplast and palisade cell development....

  4. Homozygous variegate porphyria presenting with developmental and language delay in childhood.

    Science.gov (United States)

    Pinder, V A E; Holden, S T; Deshpande, C; Siddiqui, A; Mellerio, J E; Wraige, E; Powell, A M

    2013-10-01

    Variegate porphyria is an autosomal dominant disorder that usually presents with photosensitivity and acute neurological crises in adulthood. It is caused by heterozygous mutations in the protoporphyrinogen oxidase gene (PPOX). A rarer variant, homozygous variegate porphyria (HVP), presents in childhood with recurrent skin blisters and scarring. More variable features of HVP are short stature, brachydactyly, nystagmus, epilepsy, developmental delay and mental retardation. We describe a child who presented with nystagmus, developmental delay and ataxia, combined with a photosensitive eruption. Analysis of porphyrins in plasma, urine and stool supported a clinical diagnosis of HVP. DNA from the patient showed that he is compound heterozygous for two novel missense mutations in the PPOX coding region: c.169G>C (p.Gly57Arg) and c.1259C>G (Pro420Arg). Interestingly, cranial magnetic resonance imaging showed an absence of myelin, a feature not previously reported in HVP, which expands the differential diagnosis of childhood hypomyelinating leucoencephalopathies. © 2013 British Association of Dermatologists.

  5. Stomatal Responses to Light and Drought Stress in Variegated Leaves of Hedera helix1

    Science.gov (United States)

    Aphalo, Pedro J.; Sánchez, Rodolfo A.

    1986-01-01

    Direct and indirect mechanisms underlying the light response of stomata were studied in variegated leaves of the juvenile phase of Hedera helix L. Dose response curves of leaf conductance were measured with blue and red light in leaves kept in normal or in an inverted position. In the green portions of the leaves, the sensitivity to blue light was nearly 100 times higher than that to red light. No response to red light was observed in the white portions of the leaves up to 90 micromoles per square meter per second. Red light indirectly affected leaf conductance while blue light had a direct effect. Leaf conductance was found to be more sensitive to drought stress and showed a more persistent aftereffect in the white portions of the leaves. A differential effect of drought stress on the responses to blue and red light was also observed. PMID:16664900

  6. Characterization of a new potyvirus causing mosaic and flower variegation in Catharanthus roseus in Brazil

    Directory of Open Access Journals (Sweden)

    Sheila Conceição Maciel

    2011-12-01

    Full Text Available Catharanthus roseus is a perennial, evergreen herb in the family Apocynaceae, which is used as ornamental and for popular medicine to treat a wide assortment of human diseases. This paper describes a new potyvirus found causing mosaic symptom, foliar malformation and flower variegation in C. roseus. Of 28 test-plants inoculated mechanically with this potyvirus, only C. roseus and Nicotiana benthamiana developed systemic mosaic, whereas Chenopodium amaranticolor and C. quinoa exhibited chlorotic local lesions. The virus was transmitted by Aphis gossypii and Myzus nicotianae. When the nucleotide sequence of the CP gene (768nt was compared with other members of the Potyviridae family, the highest identities varied from 67 to 76 %. For the 3' UTR (286nt, identities varied from 16.8 to 28.6 %. The name Catharanthus mosaic virus (CatMV is proposed for this new potyvirus.

  7. Effects of Elevated Ozone on Polka Dot Plant (Hypoestes phyllostachya) with Variegated Leaves.

    Science.gov (United States)

    Sui, J X; Wen, M X; Jia, L L; Chen, Y J; Li, C H; Zhang, L

    2017-10-01

    In this study, impacts of O 3 on four cultivars ('Rose', 'Pink', 'Blush' and 'White') of the polka dot plant with variegated leaves were investigated for the first time. Ozone fumigation [(120 ± 20 ppb) for 14 days (8 h day -1 , from 8:30 to 16:30)] resulted in visible foliar injuries, decreased contents of pigments (chlorophyll a and b, and carotenoid), the inhibition of photosynthesis, the increase of quantum yield of non-regulated heat dissipation and fluorescence emission (Y(NO)), and the damage of cell membrane. Elevated O 3 increased the content of anthocyanin (Ant). 'White' showed the highest, and 'Rose' the lowest amount of injured leaf area, indicating that the former was the most sensitive, and the latter the most tolerant to O 3 stress. After O 3 exposure, the highest Ant content was found in 'Rose', followed by 'Pink', 'Blush', and 'White'. Levels of Ant were likely responsible for the different sensitivities to O 3 due to their roles in photoprotection.

  8. Herbicide-induced experimental variegate porphyria in mice: tissue porphyrinogen accumulation and response to porphyrogenic drugs.

    Science.gov (United States)

    Krijt, J; Stranska, P; Maruna, P; Vokurka, M; Sanitrak, J

    1997-01-01

    Administration of oxadiazon or oxyfluorfen (1000 ppm in the diet) to male BALB/c mice for 9 days resulted in experimental porphyria, resembling the acute phase of human variegate porphyria. Urinary concentrations of 5-aminolevulinic acid and porphobilinogen reached 1500 and 3000 mumol/L, respectively. Both herbicides caused a decrease of protoporphyrinogen oxidase activity in liver and kidney. Brain protoporphyrinogen oxidase activity was not altered. Liver and kidney porphyrin content increased to 11 and 17 nmol/g, respectively (control mice, 2 nmol/g). Over 50% of liver and kidney porphyrins were in the reduced (porphyrinogen) form. Bile of oxadiazon-treated mice contained 700 nmol/mL of protoporphyrinogen (control mice, 15 nmol/mL). Porphyrin content of the trigeminal nerve increased from 1 nmol/g in control animals to 11 nmol/g in oxadiazon-treated animals, suggesting a possible contribution of peripheral nerve porphyrins to porphyric neuropathy. Mice treated with 125 ppm of oxadiazon in the diet for 9 days excreted moderately elevated levels of porphobilinogen in urine (control mice, less than 50 mumol/L; treated mice, 330 mumol/L). Administration of phenobarbital or phenytoin (single injections on days 7, 8, and 9) increased the urinary porphobilinogen concentration to 3500 mumol/L. This response to porphyrogenic drugs resembles the response observed in human acute porphyrias.

  9. Infrared spectroscopy: a potential tool in huanglongbing and citrus variegated chlorosis diagnosis.

    Science.gov (United States)

    Cardinali, Marcelo Camponez do Brasil; Villas Boas, Paulino Ribeiro; Milori, Débora Marcondes Bastos Pereira; Ferreira, Ednaldo José; França e Silva, Marina; Machado, Marcos Antonio; Bellete, Barbara Sayuri; da Silva, Maria Fatima das Graças Fernandes

    2012-03-15

    Huanglongbing (HLB) and citrus variegated chlorosis (CVC) are serious threats to citrus production and have caused considerable economic losses worldwide, especially in Brazil, which is one of the biggest citrus producers in the world. Neither disease has a cure nor an efficient means of control. They are also generally confused with each other in the field since they share similar initial symptoms, e.g., yellowing blotchy leaves. The most efficient tool for detecting these diseases is by polymerase chain reaction (PCR). However, PCR is expensive, is not high throughput, and is subject to cross reaction and contamination. In this report, a diagnostic method is proposed for detecting HLB and CVC diseases in leaves of sweet orange trees using attenuated total reflectance Fourier transform infrared spectroscopy and the induced classifier via partial least-squares regression. Four different leaf types were considered: healthy, CVC-symptomatic, HLB-symptomatic, and HLB-asymptomatic. The results show a success rate of 93.8% in correctly identifying these different leaf types. In order to understand which compounds are responsible for the spectral differences between the leaf types, samples of carbohydrates starch, sucrose, and glucose, flavonoids hesperidin and naringin, and coumarin umbelliferone were also analyzed. The concentration of these compounds in leaves may vary due to biotic stresses. Copyright © 2012 Elsevier B.V. All rights reserved.

  10. Arabidopsis VARIEGATED 3 encodes a chloroplast-targeted, zinc-finger protein required for chloroplast and palisade cell development

    DEFF Research Database (Denmark)

    Næsted, Henrik; Holm, Agnethe; Jenkins, Tom

    2004-01-01

    The stable, recessive Arabidopsis variegated 3 (var3) mutant exhibits a variegated phenotype due to somatic areas lacking or containing developmentally retarded chloroplasts and greatly reduced numbers of palisade cells. The VAR3 gene, isolated by transposon tagging, encodes the 85.9 kDa VAR3...... that pigment profiles are qualitatively similar in wild type and var3, although var3 accumulates lower levels of chlorophylls and carotenoids. These results indicate that VAR3 is a part of a protein complex required for normal chloroplast and palisade cell development....

  11. Heredity of flake- and stripe-variegated traits and their introduction into Japanese day-neutral winter-flowering sweet pea (Lathyrus odoratus L.) cultivars.

    Science.gov (United States)

    Yagishita, Yoshimi; Hara, Yasuhide; Nakayama, Masayoshi

    2018-01-01

    Sweet pea ( Lathyrus odoratus L.) is a major cut flower in Japan, generally grown in greenhouses in winter to spring. The wild-type sweet pea is a long-day summer-flowering plant. The day-neutral winter-flowering ability, which allows cut-flower production in Japan, is a recessive phenotype that emerged by spontaneous mutation. Although Japanese winter-flowering cultivars and additionally spring-flowering cultivars, which have semi-long-day flowering ability generated by crossing the winter- and summer-flowering cultivars, have superior phenotypes for cut flowers, they have limited variation in color and fragrance. In particular, variegated phenotypes do not appear in modern winter- and spring-flowering cultivars, only in summer-flowering cultivars. We try to expand the phenotypic diversity of Japanese cut flower cultivars. In the processes, we introduced the variegated phenotypes by crossing with summer-flowering cultivars, and succeeded in breeding some excellent cultivars. During breeding, we analyzed the segregation ratios and revealed the heredity of the phenotypes. Here we review the heredity of these variegated phenotypes and winter-flowering phenotypes and their related genes. We also describe how we introduced the trait into winter-flowering cultivars, tracing their pedigrees to show both phenotypes and genotypes of the progeny at each generation. This knowledge is useful for the efficient breeding of new variegated cultivars.

  12. Sexual differences in destructive capability and midgut enzyme activities in adult variegated grasshoppers Zonocerus variegatus (LINNAEUS, 1758 (Orthoptera: Pyrgomorphidae

    Directory of Open Access Journals (Sweden)

    Ademolu Kehinde O.

    2016-12-01

    Full Text Available The variegated grasshopper Zonocerus variegatus is a polyphagous insect, feeding on numerous food and cash crops. The present study aimed to investigate the sexual variations in the destructive capability of the adult insects and the composition of leaves damaged by them, as well as in the levels of midgut microbial flora and digestive enzymes (cellulase, amylase and α-glucosidase. The results showed that females consumed and caused more damage to cassava leaves than their male congeners. The leaves damaged by males contained more nutrients than those damaged by females. The gut microbial flora and enzyme assay showed that females had significantly larger colony forming units and a non-significant difference in enzyme activities. It can thus be concluded that adult females are more destructive than males.

  13. Investigating changes in basal conditions of Variegated Glacier prior to and during its 1982–1983 surge

    Directory of Open Access Journals (Sweden)

    M. Jay-Allemand

    2011-08-01

    Full Text Available Variegated Glacier (Alaska is known to surge periodically after a sufficient amount of cumulative mass balance is reached, but this observation is difficult to link with changes in the basal conditions. Here, using a 10-yr dataset, consisting of surface topography and surface velocity observations along a flow line for 25 dates, we have reconstructed the evolution of the basal conditions prior to and during the 1982–1983 surge. The model solves the full-Stokes problem along the central flow line using the finite element method. For the 25 dates of the dataset, the basal friction parameter distribution is inferred using the inverse method proposed by Arthern and Gudmundsson (2010. This method is here slightly modified by incorporating a regularisation term in the cost function to avoid short wavelength changes in the friction parameter. Our results indicate that dramatic changes in the basal conditions occurred between 1973 to 1983. Prior to the surge, periodic changes can be observed between winter and summer, with a regular increase of the sliding from 1973 to 1982. During the surge, the basal friction decreased dramatically and an area of very low friction moved from the upper part of the glacier to its terminus. Using a more complex friction law, these changes in basal sliding are then interpreted in terms of basal water pressure. Our results support that dramatic changes took place in the subglacial drainage system of Variegated Glacier, moving from a relatively efficient drainage system prior to the surge to an inefficient one during the surge. By reconstructing the water pressure evolution at the base of the glacier it is possible to propose a scenario for the hydrological history leading to the occurrence of a surge.

  14. Mutations in circularly permuted GTPase family genes AtNOA1/RIF1/SVR10 and BPG2 suppress var2-mediated leaf variegation in Arabidopsis thaliana.

    Science.gov (United States)

    Qi, Yafei; Zhao, Jun; An, Rui; Zhang, Juan; Liang, Shuang; Shao, Jingxia; Liu, Xiayan; An, Lijun; Yu, Fei

    2016-03-01

    Leaf variegation mutants constitute a unique group of chloroplast development mutants and are ideal genetic materials to dissect the regulation of chloroplast development. We have utilized the Arabidopsis yellow variegated (var2) mutant and genetic suppressor analysis to probe the mechanisms of chloroplast development. Here we report the isolation of a new var2 suppressor locus SUPPRESSOR OF VARIEGATION (SVR10). Genetic mapping and molecular complementation indicated that SVR10 encodes a circularly permuted GTPase that has been reported as Arabidopsis thaliana NITRIC OXIDE ASSOCIATED 1 (AtNOA1) and RESISTANT TO INHIBITION BY FOSMIDOMYCIN 1 (RIF1). Biochemical evidence showed that SVR10/AtNOA1/RIF1 likely localizes to the chloroplast stroma. We further demonstrate that the mutant of a close homologue of SVR10/AtNOA1/RIF1, BRASSINAZOLE INSENSITIVE PALE GREEN 2 (BPG2), can also suppress var2 leaf variegation. Mutants of SVR10 and BPG2 are impaired in photosynthesis and the accumulation of chloroplast proteins. Interestingly, two-dimensional blue native gel analysis showed that mutants of SVR10 and BPG2 display defects in the assembly of thylakoid membrane complexes including reduced levels of major photosynthetic complexes and the abnormal accumulation of a chlorophyll-protein supercomplex containing photosystem I. Taken together, our findings suggest that SVR10 and BPG2 are functionally related with VAR2, likely through their potential roles in regulating chloroplast protein homeostasis, and both SVR10 and BPG2 are required for efficient thylakoid protein complex assembly and photosynthesis.

  15. Beach almond (Terminalia catappa, Combretaceae seed production and predation by scarlet macaws (Ara macao and variegated squirrels (Sciurus variegatoides

    Directory of Open Access Journals (Sweden)

    Jonathan J. Henn

    2014-09-01

    Full Text Available Knowledge of ecological impacts of exotic beach almond (Terminalia catappa in the central Pacific of Costa Rica are little known, but studies have found this species to be a potentially important food source for endangered scarlet macaws (Ara macao. In this study, reproductive phenology and seed predation by variegated squirrels (Sciurus variegatoides and scarlet macaws were measured during March and April 2011 on beaches of central Pacific coastal Costa Rica. Seed productivity and predation levels were quantified on a weekly basis for 111 beach almond trees to assess the importance of beach almond as a food source for scarlet macaws and the extent of resource partitioning between seed predators. Seed production of the trees was great (about 194 272 seeds and approximately 67% of seeds were predated by seed predators. Macaws consumed an estimated 49% of seeds while squirrels consumed 18%. Additionally, evidence of resource partitioning between squirrels and macaws was found. Scarlet macaws preferred to feed on the northern side and edge of the canopy while squirrels preferred to feed on the southern and inside parts of the canopy. Both species ate most seeds on the ocean side of the tree. Despite the status of this tree as an exotic species, the beach almond appears to be an important resource for scarlet macaw population recovery. The resource produced by this tree should be taken into account as reforestation efforts continue in Costa Rica. Rev. Biol. Trop. 62 (3: 929-938. Epub 2014 September 01.

  16. Citrus Functional Genomics and Molecular Modeling in Relation to Citrus sinensis (Sweet Orange) Infection with Xylella fastidiosa (Citrus Variegated Chlorosis).

    Science.gov (United States)

    Dwivedi, Upendra N; Tiwari, Sameeksha; Prasanna, Pragya; Awasthi, Manika; Singh, Swati; Pandey, Veda P

    2016-08-01

    Citrus are among the economically most important fruit tree crops in the world. Citrus variegated chlorosis (CVC), caused by Xylella fastidiosa infection, is a serious disease limiting citrus production at a global scale. With availability of citrus genomic resources, it is now possible to compare citrus expressed sequence tag (EST) data sets and identify single-nucleotide polymorphisms (SNPs) within and among different citrus cultivars that can be exploited for citrus resistance to infections, citrus breeding, among others. We report here, for the first time, SNPs in the EST data sets of X. fastidiosa-infected Citrus sinensis (sweet orange) and their functional annotation that revealed the involvement of eight C. sinensis candidate genes in CVC pathogenesis. Among these genes were xyloglucan endotransglycosylase, myo-inositol-1-phosphate synthase, and peroxidase were found to be involved in plant cell wall metabolism. These have been further investigated by molecular modeling for their role in CVC infection and defense. Molecular docking analyses of the wild and the mutant (SNP containing) types of the selected three enzymes with their respective substrates revealed a significant decrease in the binding affinity of substrates for the mutant enzymes, thus suggesting a decrease in the catalytic efficiency of these enzymes during infection, thereby facilitating a favorable condition for infection by the pathogen. These findings offer novel agrigenomics insights in developing future molecular targets and strategies for citrus fruit cultivation in ways that are resistant to X. fastidiosa infection, and by extension, with greater harvesting efficiency and economic value.

  17. A triply cloned strain of xylella fastidiosa multiplies and induces symptoms of citrus variegated chlorosis in sweet orange

    Science.gov (United States)

    Li; Zreik; Fernandes; Miranda; Teixeira; Ayres; Garnier; Bov

    1999-08-01

    Xylella fastidiosa isolate 8.1.b obtained from a sweet orange tree affected by citrus variegated chlorosis in the state of Sao Paulo, Brazil, and shown in 1993 to be the causal agent of the disease, was cloned by repeated culture in liquid and on solid PW medium, yielding triply cloned strain 9a5c. The eighth and the 16th passages of strain 9a5c were mechanically inoculated into sweet orange plants. Presence of X. fastidiosa in sweet orange leaves of shoots having grown after inoculation (first-flush shoots) was detected by DAS-ELISA and PCR. Thirty-eight days after inoculation, 70% of the 20 inoculated plants tested positive, and all plants gave strong positive reactions 90 days after inoculation. Symptoms first appeared after 3 months and were conspicuous after 5 months. X. fastidiosa was reisolated from sweet orange leaves, 44 days after inoculation. These results indicate that X. fastidiosa strain 9a5c, derived from pathogenic isolate 8.1.b by triply cloning, is also pathogenic. Strain 9a5c is now used for the X. fastidiosa genome sequencing project undertaken on a large scale in Brazil.http://link. springer-ny.com/link/service/journals/00284/bibs/39n2p106.html

  18. Aspectos anatômicos da variegação na folha do cafeeiro Anatomical features of leaf variegation in coffee plants

    Directory of Open Access Journals (Sweden)

    D. M. Dedecca

    1957-01-01

    , ao mesmo tempo que foi impedida a formação de um verdadeiro parênquima paliçádico. A grande atividade da gema apical dos cafeeiros que exibem êste tipo de variegação, tal como pode ser observada pelas freqüentes modificações na sua morfologia, conduziu essas plantas a um intenso desenvolvimento vegetativo, servindo de evidência em favor da hipótese de que distúrbios vários devem ter ocorrido durante o desenvolvimento daquelas fôlhas.Two different types of leaf variegation in coffee plants are studied on the anatomical viewpoint, and their histological differences are described, when compared with normal, green leaves. In the first case, variegated leaves are normal in shape and texture, and show different variegation patterns, being histologically identical with green leaves of normal plants. The variegation seems to be due to disturbances in the chlorophyll-producing mechanism, resulting in the formation of large, green and normal chloroplasts, aad small, yellowish and abnormal chloroplasts. The distribution at random of cells having one of these two kinds of chloroplasts can be accounted for the different variegation patterns observed. In the second example, the variegation pattern is uniform throughout the entire plant, and is a more complex phenomenon, since besides being variegated the leaves present several malformations. The anatomical study has revealed that, in this case, the chloroplasts are always normal, the variegation being rather due to the presence of an 1-3-layered hialine hypodermis, which occurs right beneath the upper epidermis. The malformations, represented by abnormalities in the shape and texture of the leaf blade, are mainly due to the lackness of a true palisade parenchyma. This tissue shows all grades of disorganization in the various portions of the leaf, being sometimes present as small patches or palisade cells or lacking completely in other instances. It is suggested that disturbances of any kind should have occurred

  19. Weakener of white (Wow), a gene that modifies the expression of the white eye color locus and that suppresses position effect variegation in Drosophila melanogaster.

    Science.gov (United States)

    Birchler, J A; Bhadra, U; Rabinow, L; Linsk, R; Nguyen-Huynh, A T

    1994-08-01

    A locus is described in Drosophila melanogaster that modifies the expression of the white eye color gene. This trans-acting modifier reduces the expression of the white gene in the eye, but elevates the expression in other adult tissues. Because of the eye phenotype in which the expression of white is lessened but not eliminated, the newly described locus is called the Weakener of white (Wow). Northern analysis reveals that Wow can exert an inverse or direct modifying effect depending upon the developmental stage. Two related genes, brown and scarlet, that are coordinately expressed with white, are also affected by Wow. In addition, Wow modulates the steady state RNA level of the retrotransposon, copia. When tested with a white promoter-Alcohol dehydrogenase reporter. Wow confers the modifying effect to the reporter, suggesting a requirement of the white regulatory sequences for mediating the response. In addition to being a dosage sensitive regulator of white, brown, scarlet and copia, Wow acts as a suppressor of position effect variegation. There are many dosage sensitive suppressors of position effect variegation and many dosage-sensitive modifiers of gene expression. The Wow mutations provide evidence for an overlap between the two types of modifiers.

  20. Etiology of three recent diseases of citrus in São Paulo State: sudden death, variegated chlorosis and huanglongbing.

    Science.gov (United States)

    Bové, Joseph Marie; Ayres, Antonio Juliano

    2007-01-01

    , citrus variegated chlorosis (CVC), was observed in 1987 in the Triangulo Mineiro of Minas Gerais State and the northern and north-eastern parts of SSP. By 2000, the disease affected already 34% of the 200 million sweet orange trees in SSP. By 2005, the percentage had increased to 43%, and CVC was present in all citrus growing regions of Brazil. Electron microscopy showed that xylem-limited bacteria were present in all symptomatic sweet orange leaves and fruit tissues tested, but not in similar materials from healthy, symptomless trees. Bacteria were consistently cultured from twigs of CVC-affected sweet orange trees but not from twigs of healthy trees. Serological analyses showed the CVC bacterium to be a strain of Xylella fastidiosa. The disease could be reproduced and Koch's postulates fulfilled, by mechanically inoculating a pure culture of X. fastidiosa isolate 8.1.b into sweet orange seedlings. The genome of a CVC strain of X. fastidiosa was sequenced in SSP in the frame of a project supported by FAPESP and Fundecitrus. X. fastidiosa is the first plant pathogenic bacterium, the genome of which has been sequenced. Until recently, America was free of huanglongbing (HLB), but in March 2004 and August 2005, symptoms of the disease were recognized, respectively in the State of São Paulo (SSP) and in Florida, USA. HLB was known in China since 1870 and in South Africa since 1928. Because of its destructiveness and its rapid spread by efficient psyllid insect-vectors, HLB is probably the most serious citrus disease. HLB is caused by a phloem sieve tube-restricted Gram negative bacterium, not yet available in culture. In the 1990s, the bacterium was characterized by molecular techniques as a member of the alpha proteobacteria designated Candidatus Liberibacter africanus for the disease in Africa, and Candidatus Liberibacter asiaticus for HLB in Asia. In SSP, Ca. L. asiaticus is also present, but most of the trees are infected with a new species, Candidatus Liberibacter

  1. Development and systematic validation of qPCR assays for rapid and reliable differentiation of Xylella fastidiosa strains causing citrus variegated chlorosis.

    Science.gov (United States)

    Li, Wenbin; Teixeira, Diva C; Hartung, John S; Huang, Qi; Duan, Yongping; Zhou, Lijuan; Chen, Jianchi; Lin, Hong; Lopes, Silvio; Ayres, A Juliano; Levy, Laurene

    2013-01-01

    The xylem-limited, Gram-negative, fastidious plant bacterium Xylella fastidiosa is the causal agent of citrus variegated chlorosis (CVC), a destructive disease affecting approximately half of the citrus plantations in the State of São Paulo, Brazil. The disease was recently found in Central America and is threatening the multi-billion U.S. citrus industry. Many strains of X. fastidiosa are pathogens or endophytes in various plants growing in the U.S., and some strains cross infect several host plants. In this study, a TaqMan-based assay targeting the 16S rDNA signature region was developed for the identification of X. fastidiosa at the species level. Another TaqMan-based assay was developed for the specific identification of the CVC strains. Both new assays have been systematically validated in comparison with the primer/probe sets from four previously published assays on one platform and under similar PCR conditions, and shown to be superior. The species specific assay detected all X. fastidiosa strains and did not amplify any other citrus pathogen or endophyte tested. The CVC-specific assay detected all CVC strains but did not amplify any non-CVC X. fastidiosa nor any other citrus pathogen or endophyte evaluated. Both sets were multiplexed with a reliable internal control assay targeting host plant DNA, and their diagnostic specificity and sensitivity remained unchanged. This internal control provides quality assurance for DNA extraction, performance of PCR reagents, platforms and operators. The limit of detection for both assays was equivalent to 2 to 10 cells of X. fastidiosa per reaction for field citrus samples. Petioles and midribs of symptomatic leaves of sweet orange harbored the highest populations of X. fastidiosa, providing the best materials for detection of the pathogen. These new species specific assay will be invaluable for molecular identification of X. fastidiosa at the species level, and the CVC specific assay will be very powerful for the

  2. Circadian variation in metabolite and enzyme activities in the femoral and thoracic muscles of adult variegated grasshoppers, Zonocerus variegatus (Linnaeus, 1758 (Orthoptera: Pyrgomorphidae

    Directory of Open Access Journals (Sweden)

    Ademolu Kehinde Olutoyin

    2018-03-01

    Full Text Available The African variegated grasshopper, Zonocerus variegatus, exhibits daily variations in its feeding and destructive activities. A study to investigate circadian variation in metabolites (lipids, protein, glucose, K+, Ca2+, Mg2+, Cl− concentrations and enzymes (lipase, amylase, proteinase, α-glucosidase activities in the femoral and thoracic muscles of adult Z. variegatus was carried out by collecting samples at 06:00, 12:00, 18:00 and 24:00 hrs GMT. The four enzymes were present throughout the day in both thoracic and femoral muscles but at varying levels. Significantly (p<0.05 higher enzymes activities were measured during the day (between 06:00 and 18:00 hours GMT (except proteinase than at night (24:00 hrs. Organic substances in the two tissues were present in significantly higher concentrations during the day than at night. Similarly, significantly more inorganic substances were recorded in the afternoon (12:00-18:00 hrs than at night in both femoral and thoracic muscles. It can thus be concluded that locomotor activities in Z. variegatus reach a peak during the day.

  3. Partial protoporphyrinogen oxidase (PPOX gene deletions, due to different Alu-mediated mechanisms, identified by MLPA analysis in patients with variegate porphyria

    Directory of Open Access Journals (Sweden)

    Barbaro Michela

    2013-01-01

    Full Text Available Abstract Variegate porphyria (VP is an autosomal dominantly inherited hepatic porphyria. The genetic defect in the PPOX gene leads to a partial defect of protoporphyrinogen oxidase, the penultimate enzyme of heme biosynthesis. Affected individuals can develop cutaneous symptoms in sun-exposed areas of the skin and/or neuropsychiatric acute attacks. The identification of the genetic defect in VP families is of crucial importance to detect the carrier status which allows counseling to prevent potentially life threatening neurovisceral attacks, usually triggered by factors such as certain drugs, alcohol or fasting. In a total of 31 Swedish VP families sequence analysis had identified a genetic defect in 26. In the remaining five families an extended genetic investigation was necessary. After the development of a synthetic probe set, MLPA analysis to screen for single exon deletions/duplications was performed. We describe here, for the first time, two partial deletions within the PPOX gene detected by MLPA analysis. One deletion affects exon 5 and 6 (c.339-197_616+320del1099 and has been identified in four families, most probably after a founder effect. The other extends from exon 5 to exon 9 (c.339-350_987+229del2609 and was found in one family. We show that both deletions are mediated by Alu repeats. Our findings emphasize the usefulness of MLPA analysis as a complement to PPOX gene sequencing analysis for comprehensive genetic diagnostics in patients with VP.

  4. Variegated operation of MAPS reactors after enmasse' coolant channel replacement: a tale-tell signature of high standard fuel bundle production quality

    International Nuclear Information System (INIS)

    Jena, J.K.; Sahu, J.K.; Arularasan, V.; Sivagurnathan, D.; Rathakrishnan, S.; Ramamurthy, K.

    2009-01-01

    After the Enmasse' Coolant Channel Replacement (EMCCR) of both the reactors of Madras Atomic Power Station (MAPS), they have put up a good performance, as far as core integrity is considered. This is a tale-tell signature of the high quality of the fuel bundles manufactured by Nuclear Fuel Complex (NFC), Hyderabad. Both the reactor cores have been loaded with various types of fuel bundles viz. Natural Uranium (NU), Depleted Uranium (DU), and Deeply Depleted Uranium (DDU) and were operated at different power level with different flux configuration at different stages of operation. Even around 1026 low burn up bundle (<2500 MWD/TeU) were transferred from MAPS-1 to MAPS-2, first time in the history of PHWRS. During all such variegated operations, the Primary Heat Transport (PHT) system 131 I activity, which is synonymous with the core integrity, was maintaining low for most of the reactor operation period. However, recently a low burn up fuel bundle failure has been observed in MAPS-1. Even though the overall failure rate is very low, the cause of such failure needs to be ascertained for taking appropriate action to maintain the high standards of quality in the manufacturing process of the fuel bundles. (author)

  5. Alternation of host plants as a survival mechanism of leafhoppers Dilobopterus costalimai and Oncometopia facialis (Hemiptera: Cicadellidae, vectors of the Citrus Variegated Chlorosis (CVC

    Directory of Open Access Journals (Sweden)

    Milanez José Maria

    2001-01-01

    Full Text Available Dilobopterus costalimai (Young and Oncometopia facialis (Signoret are two of the most important species of citrus leafhoppers, vectors of bacterium Xylella fastidiosa which causes the Citrus Variegated Chlorosis (CVC disease. To develop a rearing technique for these species under laboratory conditions, the egg laying preference and nymph development were studied in different breeding systems: Rangpur lime (Citrus limonia and "falso boldo" (Vernonia condensata as host plants. Trials were set up in a randomized block design with three treatments (n=8. Females of D. costalimai had particular preference for ovipositing on Rangpur lime leaves while O. facialis females placed a higher number of eggs on "falso boldo", but it did not differ statistically from the Rangpur lime. The nymphal viability of D. costalimai was null in Rangpur lime and 58% in "falso boldo". For O. facialis the nymphal viability was 25 and 78% in Rangpur lime and "falso boldo", respectively. "Falso boldo" is more suitable as a host plant to rear the two species of citrus leafhoppers. The alternation of host plants seems to be an important survival mechanism of the CVC-vector species, as shown in natural conditions.

  6. Molybdenite Re-Os dating of Mo-Th-Nb-REE rich marbles: pre-Variscan processes in Moldanubian Variegated Group (Czech Republic

    Directory of Open Access Journals (Sweden)

    Drábek Milan

    2015-06-01

    Full Text Available In an effort to contribute to the discussion concerning the age of rocks of the Moldanubian Variegated Group, we have undertaken Re-Os dating of molybdenite of banded carbonatite-like marbles (CLM from the graphite mine Václav at Bližná (Southern Bohemia, which belong to the metamorphic sequence of this group. The Re-Os model ages for the molybdenites range between 493 and 497 Ma and apparently correspond to the early stages of metamorphism connected with pre-Variscan rift-related tectono-metamorphic events, which affected and recrystallized sedimentary CLM material rich in Mo-Th-Nb-REE. The molybdenite bearing carbonatite like marbles situated in the footwall of Bližná graphite mine have been interpreted as carbonates with a large share of volcano-detritic material derived from contemporaneous primitive alkaline (carbonatite-like volcanism deposited in a shallow marine lagoonal environment. There is no geological evidence for the participation of fluids mobilized from host rocks in the formation of the CLM. Because the Re-Os chronometer in molybdenite is demonstrably stable through later Variscan facies metamorphism, the molybdenite chronometer has not been affected by subsequent thermal overprints associated with the Variscan orogeny.

  7. Management of chimera and in vitro mutagenesis for development of new flower color/shape and chlorophyll variegated mutants in chrysanthemum

    Energy Technology Data Exchange (ETDEWEB)

    Datta, S.K. [CSIR, Madhyamgram Experimental Farm, Bose Institute, Kolkata (India)], E-mail: subodhskdatta@rediffmail.com; Chakrabarty, D [Floriculture Laboratory, National Botanical Research Institute, Lucknow (India)

    2008-07-01

    Induced mutagenesis has played a major role in the development of many new flower color/shape mutant varieties in ornamentals. The main bottleneck with vegetatively propagated plants is that the mutation appears as a chimera whether developed through bud sport or through induced mutation. The size of the mutant sector varies from a narrow streak on a petal to the entire flower and from a portion of a branch to the entire branch. When a portion of a branch or entire branch is mutated, the mutant tissue can be isolated; on the other hand, a small sector of a mutated branch or flower cannot be isolated using the available conventional propagation techniques. A novel technique has been standardized in our laboratory for the management of chimeric tissues through direct shoot regeneration from chrysanthemum florets. 'Kasturba Gandhi', a large white flowered chrysanthemum, developed few chimeric yellow florets due to spontaneous mutation. Using in vitro protocol new yellow florets were established in pure form. In vitro mutagenesis experiments were conducted treating ray florets of chrysanthemum cultivars using gamma rays. Induced chimeric yellow, white, light yellow, light mauve and dark mauve floret color sectors and chlorophyll variegation in leaves of cv. 'Maghi' (with mauve floret and green leaves) have been established in pure form. Gamma ray induced sectorial yellow florets of cv. 'Lilith' (white floret) and yellow ray florets in both the cvs. 'Purnima' (with white florets) and 'Colchi Bahar' (with red florets) have been isolated in pure form through in vitro management. Induced sectorial flower color/shape mutations in cvs. 'Puja', 'Lalima', 'Flirt', 'Maghi' and 'Sunil' have been isolated in pure form through in vitro culture. Gamma radiation procedure and tissue culture techniques have been optimized to regenerate plants from stem internodes, stem node, shoot tip and ray florets. Present technique has opened a new way for isolating new flower color

  8. JIL-1 and Su(var)3-7 Interact Genetically and Counteract Each Other's Effect on Position-Effect Variegation in Drosophila

    Science.gov (United States)

    Deng, Huai; Cai, Weili; Wang, Chao; Lerach, Stephanie; Delattre, Marion; Girton, Jack; Johansen, Jørgen; Johansen, Kristen M.

    2010-01-01

    The essential JIL-1 histone H3S10 kinase is a key regulator of chromatin structure that functions to maintain euchromatic domains while counteracting heterochromatization and gene silencing. In the absence of the JIL-1 kinase, two of the major heterochromatin markers H3K9me2 and HP1a spread in tandem to ectopic locations on the chromosome arms. Here we address the role of the third major heterochromatin component, the zinc-finger protein Su(var)3-7. We show that the lethality but not the chromosome morphology defects associated with the null JIL-1 phenotype to a large degree can be rescued by reducing the dose of the Su(var)3-7 gene and that Su(var)3-7 and JIL-1 loss-of-function mutations have an antagonistic and counterbalancing effect on position-effect variegation (PEV). Furthermore, we show that in the absence of JIL-1 kinase activity, Su(var)3-7 gets redistributed and upregulated on the chromosome arms. Reducing the dose of the Su(var)3-7 gene dramatically decreases this redistribution; however, the spreading of H3K9me2 to the chromosome arms was unaffected, strongly indicating that ectopic Su(var)3-9 activity is not a direct cause of lethality. These observations suggest a model where Su(var)3-7 functions as an effector downstream of Su(var)3-9 and H3K9 dimethylation in heterochromatic spreading and gene silencing that is normally counteracted by JIL-1 kinase activity. PMID:20457875

  9. Testing the process of drilling - analysis of drilling performance in variegated sandstone and Keuper in Northern Germany; Der Bohrprozess auf dem Pruefstand - Analyse der Bohrleistungen im Buntsandstein und Keuper in Norddeutschland

    Energy Technology Data Exchange (ETDEWEB)

    Pust, G.; Tschaffler, H. [Mobil Erdgas Erdoel GmbH, Celle (Germany); Grunwald, R. [BEB Erdgas und Erdoel GmbH, Nienhagen (Germany); Gloth, H. [TU Bergakademie Freiberg (Germany); Marx, C. [ITE, Clausthal-Zellerfeld (Germany)

    1998-12-31

    The aim of the project launched jointly by DGMK and 5 companies from the German oil- and gas industry and two institutes for drilling technologies was to find suitable ways in which the drilling progress in formations with bad drilling conditions in Northern Germany, i.e. lower and middle variegated sandstone and Keuper can be increases in order to bring costs down. The borehole sunk in Northern Germany were surveyed and inventorised. Data were subjected to a thorough statistical analysis in order to obtain optimal drilling parameters and improve the drilling tools. Basic studies on rock damaging were also to be included in order to better understand the processes. Finally, the project aims at realising the optimal drilling parameters, increase drilling progress, use improved drilling machinery and thus cut costs. (orig.) [Deutsch] Ziel des DGMK-Gemeinschaftsprojektes mit 5 Firmen der deutschen Erdoel- und Erdgasindustrie sowie 2 bohrtechnischen Instituten war es, durch geeignete Massnahmen den Bohrfortschritt in schlechtbohrbaren Formationen in Norddeutschland, naemlich unterer und mittlerer Buntsandstein sowie Keuper, zu erhoehen und damit einen Beitrag zur Kostensenkung zu leisten. Um dieses Ziel zu erreichen, sollte eine Bestandsaufnahme der in Norddeutschland abgeteuften Bohrungen erfolgen. Die Daten sollten einer eingehenden statistischen Auswertung unterzogen werden. Hieraus sollten optimale Bohrparameter und Verbesserungen der Bohrwerkzeuge abgeleitet werden. Ferner sollten grundlegende Untersuchungen zur Gesteinszerstoerung herangezogen werden, um ein besseres Verstaendnis fuer die ablaufenden Prozesse zu bekommen. Durch die Realisierung optimaler Bohrparameter und den Einsatz verbesserter Bohrwerkszeuge sollte schliesslich die Erhoehung des Bohrfortschritts und damit eine Senkung der Kosten erreicht werden. (orig.)

  10. Enhanced reliability and accuracy for field deployable bioforensic detection and discrimination of Xylella fastidiosa subsp. pauca, causal agent of citrus variegated chlorosis using razor ex technology and TaqMan quantitative PCR.

    Science.gov (United States)

    Ouyang, Ping; Arif, Mohammad; Fletcher, Jacqueline; Melcher, Ulrich; Ochoa Corona, Francisco Manuel

    2013-01-01

    A reliable, accurate and rapid multigene-based assay combining real time quantitative PCR (qPCR) and a Razor Ex BioDetection System (Razor Ex) was validated for detection of Xylella fastidiosa subsp. pauca (Xfp, a xylem-limited bacterium that causes citrus variegated chlorosis [CVC]). CVC, which is exotic to the United States, has spread through South and Central America and could significantly impact U.S. citrus if it arrives. A method for early, accurate and sensitive detection of Xfp in plant tissues is needed by plant health officials for inspection of products from quarantined locations, and by extension specialists for detection, identification and management of disease outbreaks and reservoir hosts. Two sets of specific PCR primers and probes, targeting Xfp genes for fimbrillin and the periplasmic iron-binding protein were designed. A third pair of primers targeting the conserved cobalamin synthesis protein gene was designed to detect all possible X. fastidiosa (Xf) strains. All three primer sets detected as little as 1 fg of plasmid DNA carrying X. fastidiosa target sequences and genomic DNA of Xfp at as little as 1 - 10 fg. The use of Razor Ex facilitates a rapid (about 30 min) in-field assay capability for detection of all Xf strains, and for specific detection of Xfp. Combined use of three primer sets targeting different genes increased the assay accuracy and broadened the range of detection. To our knowledge, this is the first report of a field-deployable rapid and reliable bioforensic detection and discrimination method for a bacterial phytopathogen based on multigene targets.

  11. Avaliação de tangerinas, tangores e tangelos em relação à clorose variegada dos citros Evaluation of mandarines, tangors and tangelos in relation to the citrus variegated chlorosis (CVC

    Directory of Open Access Journals (Sweden)

    Simone Rodrigues da Silva

    2004-04-01

    Full Text Available A Clorose Variegada dos Citros (CVC, causada pela bactéria Xylella fastidiosa, é uma séria ameaça à citricultura brasileira, constituindo-se, atualmente, numa das principais doenças dos citros no Brasil. O objetivo desse trabalho foi avaliar cultivares de citros introduzidas quanto à suscetibilidade ou resistência à CVC, em condições de campo. O trabalho foi conduzido em Bebedouro-SP. Os materiais genéticos estudados foram cultivares de tangerinas e híbridos (tangores e tangelos introduzidas de bancos de germoplasma da Itália, Portugal, Espanha e Córsega. O trabalho foi constituído por 56 parcelas, com três plantas das quais uma foi inoculada, empregando-se o método de encostia, utilizando-se de mudas previamente infectadas como fontes da bactéria. Foram avaliados os sintomas da doença mediante observação visual através de notas e teste de PCR, específico para Xylella fastidiosa. Os materiais genéticos que se apresentaram positivos ao PCR, mas não apresentaram sintomas, e os que foram negativos ao PCR, possuem um potencial de utilização em programas de melhoramento genético visando à resistência e/ou à tolerância à doença.The Citrus Variegated Chlorosis (CVC, caused by the bacteria Xylella fastidiosa, is a serious threat to the Brazilian citriculture, being, actually, one of the mains diseases to the citrus in Brazil. The objective of this work was evaluate citrus cultivars, introduced according to the susceptibility or resistance to CVC, in field conditions. The research was carried in Bebedouro city - São Paulo state - Brazil. The genotypes studied were cultivars of mandarines and hybrids (tangors and tangelos, introduced from the germplasm collections of Italy, Portugal, Spain and Corsica. The work was composed by 56 portions, with 3 plants, being one of them inoculated, using the approach graft method, with infected plants. Were evaluated the symptoms of the disease by visual observation and PCR test

  12. A MORPHOMETRIC STUDY OF THE VARIEGATED GRASSHOPPER

    African Journals Online (AJOL)

    BIG TIMMY

    Department of Zoology, Obafemi Awolowo University, Ile-Ife, Nigeria. Correspondence a. Morphometry of the dry-season population of adult ... female specimens from Port-Harcourt were the most correctly classified compared to specimens from other locations . The present study demonstrated the morphological similarities ...

  13. Mechanisms And Variegated Manifestations Of Inteference In ...

    African Journals Online (AJOL)

    Nigerian bilinguals' use of English as a second language is fraught with interference from L1 to L2, a phenomenon that is commonplace world-wide as a linguistic enterprise. But the extent of this trend in bilinguals varies from one language to another based on sociolinguistic and cultural variables. In Nigeria, manifestations ...

  14. Deficiência hídrica agrava os sintomas fisiológicos da clorose variegada dos citros em laranjeira 'Natal' Water deficiency intensifies physiological symptoms of citrus variegated clorosis in 'Natal' sweet orange plants

    Directory of Open Access Journals (Sweden)

    Eduardo Caruso Machado

    2007-01-01

    Full Text Available A clorose variegada dos citros (CVC é uma doença que tem promovido sérios prejuízos aos laranjais das regiões Norte e Nordeste do Estado de São Paulo, onde a deficiência hídrica e as altas temperaturas são mais frequentes. Assim, este trabalho objetivou a avaliação do efeito da deficiência hídrica no desenvolvimento de sintomas fisiológicos em laranjeira 'Natal' com CVC. Foram realizadas medidas do potencial da água na folha, transpiração, condutância estomática e assimilação de CO2, em laranjeiras em condições naturais e submetidas à irrigação. O delineamento experimental foi em blocos ao acaso com cinco repetições. A condutância estomática, a transpiração diária e o potencial da água na folha foram menores nas plantas com CVC. A assimilação diária de CO2 foi menor nas laranjeiras com CVC mesmo quando irrigadas. De fato, a irrigação diminuiu o efeito da CVC, porém não impediu o estabelecimento da doença em laranjeiras inoculadas com Xylella fastidiosa. Em relação aos demais tratamentos, as plantas infectadas e mantidas sob condições naturais (sem irrigação apresentaram maior comprometimento das trocas gasosas, mesmo quando as avaliações fisiológicas foram feitas em período úmido (verão.Citrus variegated chlorosis (CVC is a disease that has caused serious economical losses in citrus grove located in the North and Northeastern regions of São Paulo State, where water deficiency and high temperature occur frequently. Therefore, the aim of this work was to evaluate the influence of water deficiency on the development of physiological symptoms in 'Natal' sweet orange plants with CVC. Measurements of leaf water potential, transpiration, stomatal conductance e CO2 assimilation were taken in plants under natural conditions and submitted to irrigation. The experimental design was in randomized blocks, with five replications. Stomatal conductance, daily transpiration and leaf water potential were

  15. Fluxo de seiva e fotossíntese em laranjeira 'Natal' com clorose variegada dos citros Sap flow and photosynthesis of 'Natal' sweet orange plants with citrus variegated chlorosis

    Directory of Open Access Journals (Sweden)

    Eduardo Caruso Machado

    2006-06-01

    Full Text Available O objetivo deste trabalho foi avaliar os efeitos da clorose variegada dos citros (CVC, no fluxo de seiva, trocas gasosas e atividade fotoquímica em laranjeira 'Natal', com e sem CVC, em condição de campo. O curso diário do fluxo de seiva, potencial da água na folha, assimilação de CO2, transpiração, condutância estomática e eficiência quântica máxima e efetiva do fotossistema II foram avaliados. O delineamento experimental foi em blocos ao acaso com cinco repetições. O fluxo de seiva foi 1,9 vez superior nas plantas sadias em relação às doentes. Em plantas doentes ocorreu queda de 43, 28 e 33% na assimilação de CO2, condutância estomática e transpiração, respectivamente. As plantas com CVC apresentaram fotoinibição dinâmica. Uma vez que a eficiência quântica efetiva apresentou um padrão de resposta semelhante, durante o dia, em ambos os tratamentos, o efeito protetor da fotorrespiração no aparato fotoquímico em plantas com CVC é discutido. As quedas de assimilação de CO2, transpiração e de fluxo de seiva, nas plantas com CVC, foram decorrentes do menor valor da condutância estomática, possivelmente causado pela colonização dos vasos do xilema pela Xylella fastidiosa.The objective of this work was to evaluate the effects of citrus variegated chlorosis (CVC on sap flow, gas exchanges and photochemical activity in 'Natal' sweet orange plants with and without CVC under field condition. Diurnal courses of sap flow, leaf water potential, CO2 assimilation rate and transpiration, stomatal conductance, potential and effective quantum efficiency of photosystem II were evaluated. The experiment was arranged in a random block design with five repetitions. Healthy plants showed sap flow values around 1.9 times higher than injured ones. Injured plants exhibited reductions of 43, 28 and 33% in CO2 assimilation rate, stomatal conductance and leaf transpiration, respectively. CVC-affected plants showed dynamic

  16. Variegate porphyria in South Africa, 1688 - 1996 - new ...

    African Journals Online (AJOL)

    Specific suppression of haem synthesis with intravenous ... by abdominal pain, paralysis and the passage of red urine. This was .... In the marrow haem synthesis is also Iimrted by .... serum urea levels being elevated in 53% of subjects. 80th.

  17. 7 CFR 29.1081 - Variegated red or scorched (KR).

    Science.gov (United States)

    2010-01-01

    ....1081 Agriculture Regulations of the Department of Agriculture AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE COMMODITY STANDARDS AND STANDARD CONTAINER... usually results from excessive heat in the curing process. Any leaf of which 20 percent or more of its...

  18. 7 CFR 29.1080 - Variegated dark red (KD).

    Science.gov (United States)

    2010-01-01

    ... Agriculture Regulations of the Department of Agriculture AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE COMMODITY STANDARDS AND STANDARD CONTAINER... which usually results from excessive sunbaking during the growing process or from storing cured tobacco...

  19. Pulmonary hypertension’s variegated landscape: a snapshot

    Science.gov (United States)

    Kulik, Thomas J.; Austin, Eric D.

    2017-01-01

    The many types of pulmonary hypertension (PH) are so protean in their biological origin, histological expression, and natural history that it is difficult to create a summary picture of the disease, or to easily compare and contrast characteristics of one type of PH with another. For newcomers to the field, however, such a picture would facilitate a broad understanding of PH. In this paper, we suggest that four characteristics are fundamental to describing the nature of various types of PH, and that taken together they define a number of patterns of PH expression. These characteristics are histopathology, developmental origin, associated clinical conditions, and potential for resolution. The “snapshot” is a way to concisely display the ways that these signal characteristics intersect in select specific types of PH, and is an effort to summarize these patterns in a way that facilitates a “big picture” comprehension of this disease. PMID:28680566

  20. Variegated colors of pediatric glioblastoma multiforme: what to expect?

    Directory of Open Access Journals (Sweden)

    Vivek Immanuel

    2017-08-01

    Full Text Available Malignant gliomas account for 35-45% of primary brain tumors; among these glioblastoma multiforme (GBM is the most common adult brain tumor constituting approximately 85%. Its incidence is quite less in the pediatric population and treatment of these patients is particularly challenging. Exposure to ionizing radiation is the only environmental factor found to have any significant association with GBM. Several genetic alterations associated with GBM in adults have been well documented such as epidermal growth factor receptor amplification, overexpression of mouse double minute 2 homolog also known as E3 ubiquitin-protein ligase, Phosphatase and tensin homolog gene mutation, loss of heterozygosity of chromosome 10p and isocitrate dehydrogenase-1 mutation. However, data on genetic mutations in pediatric GBM is still lacking. Exophytic brain stem gliomas are rare tumors and are usually associated with a poor prognosis. The most effective treatment in achieving long-term survival in such patients, is surgical excision of the tumor and then chemoradiotherapy followed by adjuvant chemotherapy by temozolomide. This schedule is the standard treatment for GBM patients. In view of the rarity of pediatric GBM, we report here a case of pontine GBM in a 5-year-old girl.

  1. Análise espaço-temporal da clorose variegada dos citros no Noroeste do Paraná, com uso de PCR para detecção de Xylella fastidiosa = Spatio-temporal analysis of the citrus variegated chlorosis (CVC in the Northwest of Paraná, using PCR for detection of Xylella fastidiosa

    Directory of Open Access Journals (Sweden)

    William Mário de Carvalho Nunes

    2006-07-01

    Full Text Available A citricultura é afetada por inúmeras doenças, como a clorose variegada do citros (CVC, causada pela bactéria Xylella fastidiosa. O objetivo deste trabalho foi determinar a distribuição espacial da doença dentro de pomares comerciais do Noroeste do Paraná com o uso de métodos moleculares. Foram selecionados pomares sintomáticos para CVC com as variedades ‘Pêra’, ‘Valência’ e ‘Folha Murcha’ (Citrus sinensis Osbeck. Foram marcadas para cada variedade, 4 plantas-referência positivas para CVC (por sintomas e análise molecular e 8 plantas ao redor de cada uma das plantas-referência foram amostradas, num total de 36 plantas por variedade. Realizou-se o teste da Reação da Polimerase em Cadeia (PCR para detecção da bactéria e na mesma época foram conduzidas avaliações visuais de sintomas de CVC. Os resultados da análise temporal, utilizando-se os modelos Monomolecular, Logístico e Gompertz, apontaram o modelo Logístico como o que melhor se ajustou para descrever o comportamento da doença no tempo, para todas as variedades estudadas. Observou-se que o comportamento espacial da doença diferiu quando a mesma área foiavaliada pelos métodos visual e molecular, resultando em uma diferença no padrão espacial das áreas avaliadas. Portanto, ambos os métodos empregados, sintomas e PCR, foram capazes de constatar asmudanças no padrão espacial apresentado, sendo que a análise molecular (PCR foi mais sensível para detectar as mudanças ocorridas.Countless diseases affect the citriculture, as the citrus variegated chlorosis (CVC which is caused by the bacteria Xylella fastidiosa.The aim of this work was to determine the space distribution of the disease inside commercial orchards in the Northwest of Paraná, using molecular methods. Symptomatic orchards were selected for CVC with the varieties 'Pêra', 'Valência' and 'Folha Murcha' (Citrus sinensis Osbeck. For each variety, 4 positive reference-plant for CVC

  2. Nonlinear models for describing the Citrus Variegated Chlorosis in groves of two counties at northwestern Paraná state, Brazil = Modelo não-linear para a curva de progresso de incidência da Clorose Variegada dos Citros (CVC em dois municípios da região noroeste do Estado do Paraná

    Directory of Open Access Journals (Sweden)

    Clara Matiko Ueda

    2010-10-01

    Full Text Available In Brazil, the production of sweet oranges has been threatened by the Citrus Variegated Chlorosis (CVC incited by the gram-negative bacterium Xylella fastidiosa (Wells. Commercial citrus groves in two counties at the Northwestern Paraná state were evaluated to estimate the disease progression by using parameterizations of nonlinear models. Groves of Citrus sinensis Osbeck, variety “Pêra”, “Valência”, “Natal” and “Folha Murcha” had all the plants evaluated for the presence of disease symptoms. Thereafter, different parameterizations of the Logistic and Gompertz models were fitted to these data. The goodness of fit was evaluated by the intrinsic (IN and parameter-effects (PE curvatures of Bates and Watts, the bias of Box and the Hougaard measures of skewness. In Loanda, the best model was the Fermi-Dirac, and in Nova Esperança the data were best fitted to the parameterization L5, which is also a parameterization from the Logistic model.A citricultura é afetada por diversas doenças, dentre as quais a Clorose Variegada dos Citros (CVC causada pela bactéria Xylella fastidiosa (Wells. Para a região noroeste do Estado do Paraná, onde foi avaliada a CVC, propõe-se encontrar modelos não-lineares de curvas de progresso de incidência da CVC que representem o percentual de plantas acometidas pela doença. Para avaliar o comprometimento dos pomares com relação à doença, foram escolhidos pomares comerciais em dois municípios, onde foi determinada a proporção de plantas doentes. Foram selecionados talhões de laranja doce (Citrus sinensis Osbeck nas variedades “Pêra”, “Valência”, “Natal” e “Folha Murcha” e a avaliação de todas as plantas do talhão foi realizada visualmente em relação à presença ou à ausência de sintomas de CVC. Para estimar o modelo que melhor se ajustou aos dados de progresso da proporção da doença em cada talhão, foram considerados modelos não-lineares decrescimento sigmoidal

  3. Experimental investigation of the formaldehyde removal mechanisms in a dynamic botanical filtration system for indoor air purification.

    Science.gov (United States)

    Wang, Zhiqiang; Pei, Jingjing; Zhang, Jensen S

    2014-09-15

    Botanical filtration has been proved to be effective for indoor gas pollutant removal. To understand the roles of different transport, storage and removal mechanism by a dynamic botanical air filter, a series of experimental investigations were designed and conducted in this paper. Golden Pothos (Epipremnum aureum) plants was selected for test, and its original soil or activated/pebbles root bed was used in different test cases. It was found that flowing air through the root bed with microbes dynamically was essential to obtain meaningful formaldehyde removal efficiency. For static potted plant as normally place in rooms, the clean air delivery rate (CADR), which is often used to quantify the air cleaning ability of portable air cleaners, was only ∼ 5.1m(3)/h per m(2) bed, while when dynamically with air flow through the bed, the CADR increased to ∼ 233 m(3)/h per m(2) bed. The calculated CADR due to microbial activity is ∼ 108 m(3)/h per m(2) bed. Moisture in the root bed also played an important role, both for maintaining a favorable living condition for microbes and for absorbing water-soluble compounds such as formaldehyde. The role of the plant was to introduce and maintain a favorable microbe community which effectively degraded the volatile organic compounds adsorbed or absorbed by the root bed. The presence of the plant increased the removal efficiency by a factor of two based on the results from the bench-scale root bed experiments. Copyright © 2014 Elsevier B.V. All rights reserved.

  4. Active removal of ibuprofen by Money plant enhanced by ferrous ions.

    Science.gov (United States)

    Chehrenegar, Behdad; Hu, Jiangyong; Ong, Say Leong

    2016-02-01

    In this study, the removal of ibuprofen (IBP), a pharmaceutical compound, from aqueous media by Money plant (Epipremnum aureum) was investigated. The effect of ferrous iron (Fe(2+)) on enhancing the IBP removal rate was also analyzed. The first-order removal rate constants showed higher values for lower IBP initial concentrations in the range of 0.20-0.28 d(-1) for an initial concentration of 125 μg L(-1) to 0.03-0.13 d(-1) for an initial concentration of 1000 μg L(-1). Introducing ferrous iron to the aqueous media enhanced the first-order removal rate constant up to 6.5 times in a 3 d time period. Along with the removal of IBP from the media, the endogenous concentration of H2O2 also decreased presumably by the production of hydroxyl radical (·OH). Reduction in the endogenous H2O2 concentration was recorded to be 38% and 98% in the absence and presence of Fe(2+) respectively in the first day and the H2O2 level remained considerably low until day 7 which then gradually increased slightly. Simultaneous reduction of IBP and endogenous H2O2 concentration could be due to the reaction of IBP with ·OH and presumably ·OH production itself accelerated via Fenton reaction. In addition, presence of sodium bicarbonate (NaHCO3) as ·OH scavenger in the system showed reduction of first-order removal rate constant from 1.30 d(-1) to 0.07 d(-1) which could be a possible evidence of biological advanced oxidation process which is believed to play an important role in phytoremediation. Copyright © 2015 Elsevier Ltd. All rights reserved.

  5. 75 FR 17289 - Citrus Seed Imports; Citrus Greening and Citrus Variegated Chlorosis

    Science.gov (United States)

    2010-04-06

    ... Argentina and Jamaica after the October 6, 2009, Federal Import Quarantine Order was issued, we are... differing genetic histories. Nursery stock, plants, and other propagative plant material that can be..., Murraya, Poncirus, Severinia, Swinglea, Toddalia, Triphasia, and Vepris from Argentina, Bangladesh, Belize...

  6. Secondary Metabolites of the Cuticular Abdominal Glands of Variegated Grasshopper (Zonocerus variegatus L.

    Directory of Open Access Journals (Sweden)

    O. U. Igwe

    2015-01-01

    Full Text Available Chemical compounds were extracted with petroleum ether from the cuticular abdominal glands of grasshopper (Zonocerus variegatus L. and eleven compounds were characterised using Gas Chromatography/Mass Spectrometry (GC/MS technique in combination with Fourier Transform-Infrared Spectroscopy (FT-IR. The compounds analysed were 2,7-dimethyloctane (3.21%, decane (5.33%, undecane (3.81%, tridecanoic acid methyl ester (4.76%, hexadecanoic acid (9.37%, 11-octadecenoic acid methyl ester (23.18%, pentadecanoic acid, 14-methyl-methyl ester (4.43%, (Z-13-docosenoic acid (10.71%, dodecyl pentafluoropropionate (9.52%, 2-dodecyl-1,3-propanediol (6.38%, and 1,12-tridecadiene (19.30%. FT-IR analysis of the extract showed peaks at 1270.17 (C–O and C–F, 1641.48 (C=C, 2937.68 (C–H, and 3430.51 (O–H cm−1 indicating the presence of ether, alkene, alkane, alcohol, carboxylic acid, and fluoric compounds. These compounds consisted of 32.37% ester, 31.65% hydrocarbons, 20.08% fatty acid, 9.52% halogenated ester, and 6.38% alcohol. The highest component was 11-octadecenoic acid methyl ester followed by 1,12-tridecadiene. Since behavioural bioassays were not carried out, the consideration of these compounds to be pheromone semiochemicals remains a hypothesis.

  7. Nutritional deficiency in citrus with symptoms of citrus variegated chlorosis disease

    Directory of Open Access Journals (Sweden)

    ME. Silva-Stenico

    Full Text Available It is well known that citrus plants that have been infected by Xylella fastidiosa display nutritional deficiencies, probably caused by production of extracellular polymers by the bacteria that block normal nutrient flow through the xylem. The aim of this work was to study the mineral composition of specific foliar areas in different stages of infection in citrus. Thus, the concentrations of macro and micronutrients in leaves of citrus infected by X. fastidiosa were measured. Samples from four infected citrus orchards in the State of São Paulo, Brazil, were respectively collected from Santa Rita do Passa Quatro, Neves Paulista, Gavião Peixoto and Paraíso counties. The presence of X. fastidiosa in leaves was confirmed by polymerase chain reaction (PCR using specific PCR primers. To understand the variation in leaf-nutrient content in citrus plants, we used foliar nutrient values from control (non-symptomatic plants as a reference. Chemometric analysis showed that the deficiency of P and K in symptomatic trees for all orchards and high concentrations of Fe, Mn and Zn were observed in chlorotic areas, although other studies revealed deficiency of zinc in leaves. This is the first report showing that a correlation between chlorotic citrus leaf and higher concentrations of Fe, Mn and Zn are observed when infected and healthy plants were compared.

  8. Analysis of frequency effect on variegated RAM styles and other parameters using 40 nm FPGA

    DEFF Research Database (Denmark)

    Sharma, Rashmi; Pandey, Bishwajeet; Sharma, Vaashu

    2018-01-01

    . This analysis has been performed using the XILINX 12.1 and IBM SPSS Statistics 21 software and VHDL language. Pipe_distributed style at comparatively lesser values of frequencies consumes the least power. Therefore, lesser values of frequencies should be maintained while observing the power. This would bloom up...

  9. Neoliberalization, housing institutions and variegated gentrification: how the ‘third wave’ broke in Amsterdam

    NARCIS (Netherlands)

    van Gent, W.P.C.

    2013-01-01

    Neil Smith argues that in the last two decades gentrification has become a generalized global urban phenomenon. His theory is at a high level of abstraction, as it links urban gentrification to globalization, financial capitalism and neoliberalization. With these global processes, all cities have

  10. Cendrars's Variegated Poetic Persona: Seduction and Authenticity in Prose of the Transsiberian and Nineteen Elastic Poems

    Directory of Open Access Journals (Sweden)

    Everett F. Jacobus Jr.

    1979-01-01

    Full Text Available Since Cendrars recognizes the protean nature of his personal and public self, it is not surprising that the persona of his poetry escapes easy definition. My essay studies the consequences of this fact on the relationship between the poetic persona and his reader. Seduction, set against a Freudian and transactional-analysis conceptual background, provides a methodological metaphor for my analysis. In the same way that for Freud the real event of seduction only becomes psychologically effective as a fantasy and eventually as a structural pattern for the male-female relationship, our use of the seduction metaphor takes an initial naïve event between persona and reader and transforms it into a characteristic structural relationship. The persona's initial demand of intimacy from the reader is seen to be but one position in a narrative pattern which alternates the persona's attitude vis-a-vis his own narration between intimacy and detachment (or irony. The reader's role in connection with the persona is finally not one of sympathy but one of collaboration in inflecting a reading of the persona's narrated presence. Cendrars's manipulation of the persona-reader relationship is thus a primary component of his literary appeal.

  11. 76 FR 8603 - Citrus Seed Imports; Citrus Greening and Citrus Variegated Chlorosis

    Science.gov (United States)

    2011-02-15

    .../fdmspublic/component/main?main=DocketDetail&d=APHIS-2008-0052 . Comments on the interim rule were required to... that end, we cited a 2003 study \\6\\ by W.B. Li et al. (referred to below as Li et al.). \\6\\ Li, W.B., W... out that Li et al. was conducted only on three [[Page 8605

  12. CACTA-superfamily transposable element is inserted in MYB transcription factor gene of soybean line producing variegated seeds.

    Science.gov (United States)

    Yan, Fan; Di, Shaokang; Takahashi, Ryoji

    2015-08-01

    The R gene of soybean, presumably encoding a MYB transcription factor, controls seed coat color. The gene consists of multiple alleles, R (black), r-m (black spots and (or) concentric streaks on brown seed), and r (brown seed). This study was conducted to determine the structure of the MYB transcription factor gene in a near-isogenic line (NIL) having r-m allele. PCR amplification of a fragment of the candidate gene Glyma.09G235100 generated a fragment of about 1 kb in the soybean cultivar Clark, whereas a fragment of about 14 kb in addition to fragments of 1 and 1.4 kb were produced in L72-2040, a Clark 63 NIL with the r-m allele. Clark 63 is a NIL of Clark with the rxp and Rps1 alleles. A DNA fragment of 13 060 bp was inserted in the intron of Glyma.09G235100 in L72-2040. The fragment had the CACTA motif at both ends, imperfect terminal inverted repeats (TIR), inverse repetition of short sequence motifs close to the 5' and 3' ends, and a duplication of three nucleotides at the site of integration, indicating that it belongs to a CACTA-superfamily transposable element. We designated the element as Tgm11. Overall nucleotide sequence, motifs of TIR, and subterminal repeats were similar to those of Tgm1 and Tgs1, suggesting that these elements comprise a family.

  13. Variegated Voices - power, dominance and inequality: A Critical Discourse Analysis of the 2017 general elections in Kenya

    DEFF Research Database (Denmark)

    Owiso, Michael Omondi

    2018-01-01

    Public perceptions through various social media such as the WhatsApp, Twitter and Facebook as well as general public conversations in places of worship, bars, market places, churches, streets, funerals, public gatherings and interest group meetings during the election period of 2017 are a reflect......Public perceptions through various social media such as the WhatsApp, Twitter and Facebook as well as general public conversations in places of worship, bars, market places, churches, streets, funerals, public gatherings and interest group meetings during the election period of 2017...

  14. Transcriptional Adaptor ADA3 of Drosophila melanogaster Is Required for Histone Modification, Position Effect Variegation, and Transcription▿ †

    OpenAIRE

    Grau, Benjamin; Popescu, Cristina; Torroja, Laura; Ortuño-Sahagún, Daniel; Boros, Imre; Ferrús, Alberto

    2007-01-01

    The Drosophila melanogaster gene diskette (also known as dik or dAda3) encodes a protein 29% identical to human ADA3, a subunit of GCN5-containing histone acetyltransferase (HAT) complexes. The fly dADA3 is a major contributor to oogenesis, and it is also required for somatic cell viability. dADA3 localizes to chromosomes, and it is significantly reduced in dGcn5 and dAda2a, but not in dAda2b, mutant backgrounds. In dAda3 mutants, acetylation at histone H3 K9 and K14, but not K18, and at hist...

  15. Genetic background of resistance to gall mite in Ribes species

    Directory of Open Access Journals (Sweden)

    Ingrida Mazeikiene

    2017-06-01

    Full Text Available Resistance to gall mite is an important genetic trait of Ribes. P and Ce genes, responsible for gall mite resistance, were established in Ribes species and interspecific hybrids using molecular markers. Resistance in R. americanum is determined by P gene and in R. sanguineum by Ce gene. Both molecular markers were absent in R. dikuscha genome. Molecular markers related to P and Ce genes were identified in the genome of R. aureum. Resistance to gall mite in the field conditions in R. nigrum x R. americanum, R. nigrum x R. aureum and R. nigrum x R. sanguineum F3 hybrids fitted an expected Mendelian segregation ratio of 1:1, 3:1 and 1:1, respectively. 75.0% of hybrids with a pyramidal resistance to gall mite carrying markers related to Ce and P genes were obtained in the cross combination R. nigrum x R. aureum and will be included in the future breeding programs.

  16. ENVISION: A Study to Evaluate the Efficacy and Safety of Givosiran (ALN-AS1) in Patients With Acute Hepatic Porphyrias (AHP)

    Science.gov (United States)

    2018-05-21

    Acute Hepatic Porphyria; Acute Intermittent Porphyria; Porphyria, Acute Intermittent; Acute Porphyria; Hereditary Coproporphyria (HCP); Variegate Porphyria (VP); ALA Dehydratase Deficient Porphyria (ADP)

  17. Tolerating Toxins: Grasshoppers that Feast on Pyrrolizidine Alkaloids §.

    Science.gov (United States)

    Housecroft, Catherine E

    2018-03-30

    The elegant grasshopper (Zonocerus elegans) and the variegated grasshopper (Z. variegatus) are among insects that deliberately consume and store pyrrolizidine alkaloids which are subsequently used in defence mechanisms.

  18. Fulltext PDF

    Indian Academy of Sciences (India)

    GENERAL ARTICLES. 06 The Enigma of Cosmic Rays – 1. Biman Nath. 18 Theorema Aureum – 2. Shivam Kumar. 30 Solid Acid Catalysts in Green Chemistry. Some Practical Examples. Leena Rao. 37 Silicon Photonics. Silicon Raman Lasers. P K Basu. 47 Genetic Engineering of Insects. R Asokan. 57 Odd Behaviour of ...

  19. Distribution and diversity of mangrove species in Gokana Local ...

    African Journals Online (AJOL)

    Plots 1 and 2 were dominated by Rhizophora racemosa (62.07% and 41.17% respectively). Plots 3 were dominated by R. mangle (4 1.67%) while Plots 4 had 26.56% of Acrostichum aureum and 26.56% of Phoenix reclinata. The overlapping mangrove species occurrence (Laguncularia racemosa and R. mangle) at the ...

  20. Environmental Impact Analysis Process. Final Environmental Impact Statement. Supersonic Flight Operations in the Reserve Military Operations Area, Holloman, New Mexico

    Science.gov (United States)

    1983-01-01

    Juniper, one-seed Juniperus monosperma Juniper, rocky mountain Juniperus scopulorum Oak, Gambel Querqus gambeli Oak, gray Quercus grisea Oak, silverleaf... Quercus hypoleucoides Pine, Chihuahua (localized) Pinus leiophylla Pine, pinyon Pinus edulis Pine, ponderosa Pinus ponderosa Pine, southwestern white...stansburiana Currant, Golden Ribes aureum Grape, Canyon Vitis Arizonica Groundsel, Ragwort Senecio multicapitatus Morning Glory, Red Starflower Ipomea

  1. 7 CFR 29.2408 - Rule 17.

    Science.gov (United States)

    2010-01-01

    ... Regulations of the Department of Agriculture AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing... percent of mixed color or variegated leaves or over 30 percent of mixed color and variegated leaves combined shall be classified as “mixed” and designated by the color symbol “M.” ...

  2. 7 CFR 29.2632 - Rule 16.

    Science.gov (United States)

    2010-01-01

    ... Regulations of the Department of Agriculture AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing... percent of mixed color or variegated leaves or over 30 percent of mixed color and variegated leaves combined shall be classified as “mixed” and designated by the color symbol “M.” ...

  3. Flutuação populacional de espécies de cigarrinhas transmissoras da clorose variegada dos citros (CVC em Viçosa-MG Population dynamics of sharpshooters species transmitter of citrus variegated chlorosis (CVC in Viçosa-MG

    Directory of Open Access Journals (Sweden)

    Dierlei dos Santos

    2005-08-01

    Full Text Available Os objetivos deste trabalho foram avaliar a presença e a dinâmica populacional, ao longo do ano, de cigarrinhas transmissoras da CVC, no município de Viçosa - MG. As coletas foram realizadas no período de agosto de 2003 a julho de 2004, com auxílio de armadilhas adesivas amarelas e com rede entomológica (puçá. Das onze espécies comprovadamente capazes de transmitir a CVC, foram encontradas nove. O número total de cigarrinhas capturadas foi de 2.966, sendo 2.805 nas armadilhas e 161 no puçá. As três espécies da família Cicadellidae mais expressivas em número de insetos foram Bucephalogonia xanthophis (36,6% capturados na rede entomológica e 34,7% na armadilha, Dilobopterus costalimai (9,9% capturados na rede entomológica e 11,3% na armadilha e Acrogonia citrina (6,8% capturados na rede entomológica e 7,4% na armadilha. No mês de novembro, foram coletadas 378 cigarrinhas, correspondendo ao mês de maior incidência, sendo que 292 dessas foram coletadas na primeira quinzena, concorrendo para um pico populacional. Não houve diferença significativa entre o número médio de cigarrinhas coletadas no interior e na periferia da área.The objectives of this work were to evaluate the presence and the population dynamics of Citrus CVC transmitter sharpshooters along the year in the municipality of Viçosa- MG. The sharpshooter populations were monitored in the period of August 2003 to July 2004 using yellow adhesive traps and entomologic nets. During this period 2966 sharpshooters were captured and identified. Among eleven species known as CVC transmitters, nine were found in the region. The three most expressive species in number found were Bucephalogonia xanthophis (36,6% captured in entomological net and 34,7% in the trap, Dilobopterus costalimai (9,9% captured in entomological net and 11,3% in the trap and Acrogonia citrina (6,8% captured in entomological net and 7,4% in the trap. The largest incidence of sharpshooters occurred in November (378 insects, with the population reaching the pick in the first fifteen days of the month (292 insects. There was not significant difference among the medium number of sharpshooters collected in the interior and in the periphery of the area.

  4. A preliminary study on the phytoremediation of antibiotic contaminated sediment.

    Science.gov (United States)

    Hoang, Thuy Thi Thanh; Tu, Loan Thi Cam; Le Nga, Phi; Dao, Quoc Phu

    2013-01-01

    In Vietnam's coastal wetlands, fluoroquinolones, a widely used class of antibiotics in shrimp farming, are frequently detected in sediments of former shrimp farms. This phenomenon could lead to negative impacts on the aquatic ecosystem, since the antibiotic residues could induce changes in the microorganism communities of the water body. The potential of native wetland plants (Acrostichum aureum L. and Rhizophora apiculata Blume Fl. Javae) for phytoremediation of fluoroquinolones (ciprofloxacin and norfloxacin) was investigated. The half-life for each antibiotic was estimated at approximately 10 days in the planted sediment. With respect to the accumulation of ciprofloxacin and norfloxacin in plants, these antibiotics were found mainly in roots. Antibiotic translocation from root to stem and leaves occurred at a low rate. The results showed that A. aureum and R. apiculata can be valuable for the phytoremediation of antibiotic-contaminated sediments. Additionally, the initialfindings of the presence of resistant bacteria indicated that bacteria could play a role in facilitating the phytodegradation.

  5. Mutations in ash1 and trx enhance P-element-dependent silencing in Drosophila melanogaster.

    Science.gov (United States)

    McCracken, Allen; Locke, John

    2016-08-01

    In Drosophila melanogaster, the mini-w(+) transgene in Pci is normally expressed throughout the adult eye; however, when other P or KP elements are present, a variegated-eye phenotype results, indicating random w(+) silencing during development called P-element-dependent silencing (PDS). Mutant Su(var)205 and Su(var)3-7 alleles act as haplo-suppressors/triplo-enhancers of this variegated phenotype, indicating that these heterochromatic modifiers act dose dependently in PDS. Previously, we recovered a spontaneous mutation of P{lacW}ci(Dplac) called P{lacW}ci(DplacE1) (E1) that variegated in the absence of P elements, presumably due to the insertion of an adjacent gypsy element. From a screen for genetic modifiers of E1 variegation, we describe here the isolation of five mutations in ash1 and three in trx that enhance the E1 variegated phenotype in a dose-dependent and cumulative manner. These mutant alleles enhance PDS at E1, and in E1/P{lacW}ci(Dplac), but suppress position effect variegation (PEV) at In(1)w(m)(4). This opposite action is consistent with a model where ASH1 and TRX mark transcriptionally active chromatin domains. If ASH1 or TRX function is lost or reduced, heterochromatin can spread into these domains creating a sink that diverts heterochromatic proteins from other variegating locations, which then may express a suppressed phenotype.

  6. Bacterial Endophytes Isolated from Plants in Natural Oil Seep Soils with Chronic Hydrocarbon Contamination

    OpenAIRE

    Lumactud, Rhea; Shen, Shu Yi; Lau, Mimas; Fulthorpe, Roberta

    2016-01-01

    The bacterial endophytic communities of four plants growing abundantly in soils highly contaminated by hydrocarbons were analyzed through culturable and and culture-independent means. Given their tolerance to the high levels of petroleum contamination at our study site, we sought evidence that Achillea millefolium, Solidago canadensis, Trifolium aureum and Dactylis glomerata support high levels of hydrocarbon degrading endophytes. A total of 190 isolates were isolated from four plant species....

  7. Study of propagation of Berberis thunbergii L. by cuttings, with using less-known methods of stimulation

    Directory of Open Access Journals (Sweden)

    Martin Říha

    2007-01-01

    Full Text Available The different type of own produce stimulators were tested at Berberis thunbergii L. 'Green Carpet', Berberis thunbergii 'Red Shift' and Berberis thunbergii 'Aureum'. We used the combination of growing inhibitors and quick-dip method, single quick-dip metod in solution of acetone and stimulant in form of gel. Groving inhibitors is including paclobutrazol and CCC in test. We used IBK, NAA, IAA and nicotin acid as auxins in quick-dip method. Medium was aceton solution.

  8. Disease: H00995 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available d vitamin K-dependent clotting factors deficiency (VKCFD) is a rare congenital bleeding disorder consisting ...otein S, and protein Z. VKCFD leads to a bleeding tendency with a variegate clini

  9. Somatically segregating clone of apomictic maize-tripsacum hybrid

    International Nuclear Information System (INIS)

    Yudin, B.F.; Lukina, L.A.

    1988-01-01

    The results of further study on clone AM-5, isolated in the progeny of γ-irradiated plants of the apomictic hybrid of maize with tripsacum (2n = 38) are reported. The variegated-leaf seedlings of the clone segregate somatically and produce variegated, mottled, green (phenotypically normal) plants in different ratios in the apomictic progenies. The variegated, and to a lesser degree, green segregants segregate further. The mottled apomictics as well as mottled branches of variegated seedlings maintain their phenotype on transplantation, however, these is a progressive enhancement of the characters of vegetative lethality. Lethals of two extra maize genomes to the AM-5 nucleus does not affect significantly the scope and nature of segregation. At the same time, the loss of tripsacum genome restores normal phenotype. Clone AM-5 is an example of hybrid apomictic form causing significant morphological variability, which is, nevertheless, not related with apomictic and reversion to the sexual process

  10. Diversity of endophytic bacterial populations and their interaction with Xylella fastidiosa in citrus plants

    NARCIS (Netherlands)

    Araujo, W.L.; Marcon, J.; jr. Maccheroni, W.; Elsas, van J.D.; Vuurde, van J.W.L.; Azevedo, de J.L.

    2002-01-01

    Citrus variegated chlorosis (CVC) is caused by Xylella fastidiosa, a phytopathogenic bacterium that can infect all Citrus sinensis cultivars. The endophytic bacterial communities of healthy, resistant, and CVC-affected citrus plants were studied by using cultivation as well as

  11. An ENU mutagenesis screen identifies novel and known genes involved in epigenetic processes in the mouse

    Czech Academy of Sciences Publication Activity Database

    Daxinger, L.; Harten, S.K.; Oey, H.; Epp, Trevor; Isbel, L.; Huang, E.; Whitelaw, N.; Apedaile, A.; Sorolla, A.; Yong, J.; Bharti, V.; Sutton, J.; Ashe, A.; Pang, Z.Y.; Wallace, N.; Gerhardt, D.J.; Blewitt, M.E.; Jeddeloh, J.A.; Whitelaw, E.

    2013-01-01

    Roč. 14, č. 9 (2013) ISSN 1465-6906 Institutional support: RVO:68378050 Keywords : epigenetics * variegation * forward genetics Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 10.465, year: 2013

  12. 7 CFR 331.3 - PPQ select agents and toxins.

    Science.gov (United States)

    2010-01-01

    ...) Select agents and toxins listed in paragraph (b) of this section that have been genetically modified. (d... variegated chlorosis strain). (c) Genetic elements, recombinant nucleic acids, and recombinant organisms: (1...

  13. Diet and Nutrition in Porphyria

    Science.gov (United States)

    ... The so-called acute porphyrias, which are Acute Intermittent Porphyria , Hereditary Coproporphyria , Variegate Porphyria and ALAD Porphyria ... precursor substances registers changes between the fed and fasting states. The normal fast that occurs between meals ...

  14. Antimicrobial and antioxidant activities of Cortex Magnoliae Officinalis and some other medicinal plants commonly used in South-East Asia

    Directory of Open Access Journals (Sweden)

    Weng Wanyu

    2008-11-01

    Full Text Available Abstract Background Eight medicinal plants were tested for their antimicrobial and antioxidant activities. Different extraction methods were also tested for their effects on the bioactivities of the medicinal plants. Methods Eight plants, namely Herba Polygonis Hydropiperis (Laliaocao, Folium Murraya Koenigii (Jialiye, Rhizoma Arachis Hypogea (Huashenggen, Herba Houttuyniae (Yuxingcao, Epipremnum pinnatum (Pashulong, Rhizoma Typhonium Flagelliforme (Laoshuyu, Cortex Magnoliae Officinalis (Houpo and Rhizoma Imperatae (Baimaogen were investigated for their potential antimicrobial and antioxidant properties. Results Extracts of Cortex Magnoliae Officinalis had the strongest activities against M. Smegmatis, C. albicans, B. subtilis and S. aureus. Boiled extracts of Cortex Magnoliae Officinalis, Folium Murraya Koenigii, Herba Polygonis Hydropiperis and Herba Houttuyniae demonstrated greater antioxidant activities than other tested medicinal plants. Conclusion Among the eight tested medicinal plants, Cortex Magnoliae Officinalis showed the highest antimicrobial and antioxidant activities. Different methods of extraction yield different spectra of bioactivities.

  15. Antimicrobial and antioxidant activities of Cortex Magnoliae Officinalis and some other medicinal plants commonly used in South-East Asia

    Science.gov (United States)

    Chan, Lai Wah; Cheah, Emily LC; Saw, Constance LL; Weng, Wanyu; Heng, Paul WS

    2008-01-01

    Background Eight medicinal plants were tested for their antimicrobial and antioxidant activities. Different extraction methods were also tested for their effects on the bioactivities of the medicinal plants. Methods Eight plants, namely Herba Polygonis Hydropiperis (Laliaocao), Folium Murraya Koenigii (Jialiye), Rhizoma Arachis Hypogea (Huashenggen), Herba Houttuyniae (Yuxingcao), Epipremnum pinnatum (Pashulong), Rhizoma Typhonium Flagelliforme (Laoshuyu), Cortex Magnoliae Officinalis (Houpo) and Rhizoma Imperatae (Baimaogen) were investigated for their potential antimicrobial and antioxidant properties. Results Extracts of Cortex Magnoliae Officinalis had the strongest activities against M. Smegmatis, C. albicans, B. subtilis and S. aureus. Boiled extracts of Cortex Magnoliae Officinalis, Folium Murraya Koenigii, Herba Polygonis Hydropiperis and Herba Houttuyniae demonstrated greater antioxidant activities than other tested medicinal plants. Conclusion Among the eight tested medicinal plants, Cortex Magnoliae Officinalis showed the highest antimicrobial and antioxidant activities. Different methods of extraction yield different spectra of bioactivities. PMID:19038060

  16. The iojap gene in maize

    Energy Technology Data Exchange (ETDEWEB)

    Martienssen, Robert

    2001-12-01

    The classical maize mutant iojap (Iodent japonica) has variegated green and white leaves. Green sectors have cells with normal chloroplasts whereas white sectors have cells where plastids fail to differentiate. These mutant plastids, when transmitted through the female gametophyte, do not recover in the presence of wild type Iojap. We cloned the Ij locus, and we have investigated the mechanism of epigenetic inheritance and phenotypic expression. More recently, a modifier of this type of variegation, ''Inhibitor of striate'', has also been cloned. Both the iojap and inhibitor of striate proteins have homologs in bacteria and are members of ancient conserved families found in multiple species. These tools can be used to address fundamental questions of inheritance and variegation associated with this classical conundrum of maize genetics. Since the work of Rhoades there has been considerable speculation concerning the nature of the Iojap gene product, the origin of leaf variegation and the mechanism behind the material inheritance of defective plastids. This has made Iojap a textbook paradigm for cytoplasmic inheritance and nuclear-organellar interaction for almost 50 years. Cloning of the Iojap gene in maize, and homologs in other plants and bacteria, provides a new means to address the origin of heteroplastidity, variegation and cytoplasmic inheritance in higher plants.

  17. Proteomic Analysis Reveals the Leaf Color Regulation Mechanism in Chimera Hosta "Gold Standard" Leaves.

    Science.gov (United States)

    Yu, Juanjuan; Zhang, Jinzheng; Zhao, Qi; Liu, Yuelu; Chen, Sixue; Guo, Hongliang; Shi, Lei; Dai, Shaojun

    2016-03-08

    Leaf color change of variegated leaves from chimera species is regulated by fine-tuned molecular mechanisms. Hosta "Gold Standard" is a typical chimera Hosta species with golden-green variegated leaves, which is an ideal material to investigate the molecular mechanisms of leaf variegation. In this study, the margin and center regions of young and mature leaves from Hosta "Gold Standard", as well as the leaves from plants after excess nitrogen fertilization were studied using physiological and comparative proteomic approaches. We identified 31 differentially expressed proteins in various regions and development stages of variegated leaves. Some of them may be related to the leaf color regulation in Hosta "Gold Standard". For example, cytosolic glutamine synthetase (GS1), heat shock protein 70 (Hsp70), and chloroplastic elongation factor G (cpEF-G) were involved in pigment-related nitrogen synthesis as well as protein synthesis and processing. By integrating the proteomics data with physiological results, we revealed the metabolic patterns of nitrogen metabolism, photosynthesis, energy supply, as well as chloroplast protein synthesis, import and processing in various leaf regions at different development stages. Additionally, chloroplast-localized proteoforms involved in nitrogen metabolism, photosynthesis and protein processing implied that post-translational modifications were crucial for leaf color regulation. These results provide new clues toward understanding the mechanisms of leaf color regulation in variegated leaves.

  18. Bacterial Endophytes Isolated from Plants in Natural Oil Seep Soils with Chronic Hydrocarbon Contamination.

    Science.gov (United States)

    Lumactud, Rhea; Shen, Shu Yi; Lau, Mimas; Fulthorpe, Roberta

    2016-01-01

    The bacterial endophytic communities of four plants growing abundantly in soils highly contaminated by hydrocarbons were analyzed through culturable and culture-independent means. Given their tolerance to the high levels of petroleum contamination at our study site, we sought evidence that Achillea millefolium, Solidago canadensis, Trifolium aureum, and Dactylis glomerata support high levels of hydrocarbon degrading endophytes. A total of 190 isolates were isolated from four plant species. The isolates were identified by partial 16S rDNA sequence analysis, with class Actinobacteria as the dominant group in all species except S. canadensis, which was dominated by Gammaproteobacteria. Microbacterium foliorum and Plantibacter flavus were present in all the plants, with M. foliorum showing predominance in D. glomerata and both endophytic bacterial species dominated T. aureum. More than 50% of the isolates demonstrated degradative capabilities for octanol, toluene, naphthalene, kerosene, or motor oil based on sole carbon source growth screens involving the reduction of tetrazolium dye. P. flavus isolates from all the sampled plants showed growth on all the petroleum hydrocarbons (PHCs) substrates tested. Mineralization of toluene and naphthalene was confirmed using gas-chromatography. 16S based terminal restriction fragment length polymorphism analysis revealed significant differences between the endophytic bacterial communities showing them to be plant host specific at this site. To our knowledge, this is the first account of the degradation potential of bacterial endophytes in these commonly occurring pioneer plants that were not previously known as phytoremediating plants.

  19. Bacterial endophytes isolated from plants in natural oil seep soils with chronic hydrocarbon contamination

    Directory of Open Access Journals (Sweden)

    Rhea eLumactud

    2016-05-01

    Full Text Available The bacterial endophytic communities of four plants growing abundantly in soils highly contaminated by hydrocarbons were analyzed through culturable and and culture-independent means. Given their tolerance to the high levels of petroleum contamination at our study site, we sought evidence that Achillea millefolium, Solidago canadensis, Trifolium aureum and Dactylis glomerata support high levels of hydrocarbon degrading endophytes. A total of 190 isolates were isolated from four plant species. The isolates were identified by partial 16S rDNA sequence analysis, with class Actinobacteria as the dominant group in all species except Solidago canadensis, which was dominated by Gammaproteobacteria. Microbacterium foliorum and Plantibacter flavus were present in all the plants, with M. foliorum showing predominance in D. glomerata and both endophytic bacterial species dominated T. aureum. More than 50% of the isolates demonstrated degradative capabilities for octanol, toluene, naphthalene, kerosene or motor oil based on sole carbon source growth screens involving the reduction of tetrazolium dye. P. flavus isolates from all the sampled plants showed growth on all the petroleum hydrocarbons substrates tested. Mineralization of toluene and naphthalene was confirmed using gas-chromatography. 16S based terminal restriction fragment length polymorphism analysis revealed significant differences between the endophytic bacterial communities showing them to be plant host specific at this site. To our knowledge, this is the first account of the degradation potential of bacterial endophytes in these commonly occurring pioneer plants that were not previously known as phytoremediating plants.

  20. Ethnic Diversity and Political Attitudes in a Nigerian University

    Science.gov (United States)

    Brier, Alan; Tansey, Stephen

    1974-01-01

    A survey of attitudes of the student population at Nigeria's University of Ife, at the outset of the civil war in 1967, revealed the development of a common set of political attitudes among the students which are variegated but in which variations are not related to tribal origins in any fundamental aspects. (EH)

  1. Constituting Market Citizenship: Regulatory State, Market Making and Higher Education

    Science.gov (United States)

    Jayasuriya, Kanishka

    2015-01-01

    The paper makes three claims: first that regulatory state making and market making in higher education is intertwined through a project of market citizenship that shapes the "publicness" of higher education. Second, we argue that these projects of market citizenship are variegated and in Australia has taken the form of accommodation--via…

  2. Bringing the Offshore Ashore : Transnational Production, Industrial Relations and the Reconfiguration of Sovereignty

    NARCIS (Netherlands)

    Lillie, Nathan

    This article argues that off-shore is losing its exceptionality by being absorbed into a broader process of variegation and deterritorialization of sovereignty, and that capital's search for a new "fix" is driving this process. Capital goes off-shore by exploiting non-territorial definitions of

  3. Mobile Screens: The Visual Regime of Navigation

    NARCIS (Netherlands)

    Verhoeff, N.

    2012-01-01

    In this book on screen media, space, and mobility I compare synchronically, as well as diachronically, diverse and variegated screen media - their technologies and practices – as sites for virtual mobility and navigation. Mobility as a central trope can be found on the multiple levels that are

  4. The endogenous transposable element Tgm9 is suitable for functional analyses of soybean genes and generating novel mutants for genetic improvement of soybean

    Science.gov (United States)

    In soybean, variegated flowers can be caused by somatic excision of the CACTA-type transposable element Tgm9 from intron 2 of the DFR2 gene encoding dihydroflavonol-4-reductase in the anthocyanin pigment biosynthetic pathway. DFR2 has been mapped to the W4 locus where the allele containing the elem...

  5. Author Details

    African Journals Online (AJOL)

    Ezeakacha, FN. Vol 5, No 1 (2011) - Articles A Study on the Feeding and Growth Patterns of the Variegated Grasshopper Zonocerus Variegatus (L) in the Laboratory Abstract PDF. ISSN: 2070-0083. AJOL African Journals Online. HOW TO USE AJOL... for Researchers · for Librarians · for Authors · FAQ's · More about AJOL ...

  6. Author Details

    African Journals Online (AJOL)

    Development of a trap to contaminate variegated grasshoppers (Zonocerus variegatus L.) (Orthoptera: Pyrgomorphidae) with Metarrhyzium flavo-viride Gams & Rozsypal in the field. Details · Vol 40, No 1 (2007) - Articles Yam pests in the Ashanti and Brong Ahafo regions of Ghana: A study of farmers\\' indigenous technical ...

  7. Global Journal of Pure and Applied Sciences - Vol 8, No 4 (2002)

    African Journals Online (AJOL)

    The microbial flora of the different gut regions of the variegated grasshopper Zonocerus variegatus (L) (Orthoptera :Pyrgomorphidae) · EMAIL FULL TEXT EMAIL FULL TEXT · DOWNLOAD FULL TEXT DOWNLOAD FULL TEXT. A.B. Idowu, M. O. Edema, 447-454. http://dx.doi.org/10.4314/gjpas.v8i4.15992 ...

  8. Author Details

    African Journals Online (AJOL)

    A Study on the Feeding and Growth Patterns of the Variegated Grasshopper Zonocerus Variegatus (L) in the Laboratory Abstract PDF · Vol 5, No 1 (2011) - Articles Comparative Studies on the Larvicidal Action of Novaluron (Mosquiron® 100EC) and Moringa Oliefera (LAM) Seed Oil against Aedes Aegypti (Diptera: ...

  9. Insect pests' incidence and variations due to forest landscape ...

    African Journals Online (AJOL)

    No differences were found between the intensification zones, except for weeds, which appeared to be a greater constraint in the slightly degraded area. Within the insect pest, the greatest damage to crops according to farmers originated from borers and scales, followed by variegated grasshopper. Only the termites showed ...

  10. Review: Biological imprinting: Some genetic considerations | Saad ...

    African Journals Online (AJOL)

    ... as for interpretation of possible mechanisms implicated in its occurrence. Keywords: Genetic imprinting; Mutations; Re-sense mutation; Epigenetic alterations; DNA methylation/demethylation; Parthenogenesis; Position-effect variegation; Post-fertilization genomic imprinting; microRNA; Chromatin modifications; Pyknons ...

  11. 13 Tribute Diala_Brink WEB 03.pmd

    African Journals Online (AJOL)

    Owner

    aloneness, defeat, and death, the discriminations of race are revealed to be hollow as all humans, in their full variegated complexions, men and women, are shown to be ... image in political terms as the Fanonian wretched of the earth, it is often conflated with the image of Sisyphus, irrepressibly rebellious in his servitude; ...

  12. A Conceptual Framework to Explain the Role of Social Environment and Multicultural Personality on Intercultural Communication Competency, Inter Role Congruence and Interaction Comfort

    OpenAIRE

    Omar, Rosmini; Ihtiyar, Ali; Ahmad, Fauziah Sh.

    2015-01-01

    The multicultural markets require marketers to gain strong interculturalcommunication competency (ICC). Several earlier studies have emphasized thesignificance of ICC in marketing. However, empirical investigations of the subjectare scarce and inconclusive. The exceptional characteristic of Malaysia with itsexceedingly variegated multicultural and combined ethnic mix makes it one of theproper examples for ICC research and analysis to explain its antecedents andconsequence in retailing industr...

  13. The Contribution of food plants to the growth, development and ...

    African Journals Online (AJOL)

    The Contribution of food plants to the growth, development and fecundity of Zonocerus variegatus (L) ... African Journal of Biotechnology ... The performance of the variegated grasshopper, Zonocerus variegatus (L) fed on different food plants namely cassava (Manihot esculenta), pawpaw (Carica papaya) and acalypha ...

  14. South African Medical Journal - Vol 87, No 6 (1997)

    African Journals Online (AJOL)

    Variegate porphyria in South Africa, 1688 - 1996 - new developments in an old disease · EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT · DOWNLOAD FULL TEXT DOWNLOAD FULL TEXT. Richard J. Hift, Peter N. Meissner, Anne V. Corrigall, Mel R. Ziman, Lavinia A. Petersen, Doreen M. Meissner, Brandon P.

  15. Physiological roles of plastid terminal oxidase in plant stress

    Indian Academy of Sciences (India)

    The absence of PTOX in plants usually results in photo-bleached variegated leaves and impaired adaptation to environment alteration. Although PTOX level and activity has been found to increase under a wide range of stress conditions, the functions of plant PTOX in stress responses are still disputed now. In this paper ...

  16. The histone methyltransferase and putative oncoprotein MMSET is overexpressed in a large variety of human tumors

    DEFF Research Database (Denmark)

    Hudlebusch, Heidi Rye; Santoni-Rugiu, Eric; Simon, Ronald

    2011-01-01

    Multiple myeloma SET (Suppressor of variegation, Enhancer of zeste, and Trithorax) domain (MMSET) is a histone lysine methyltransferase deregulated in a subgroup of multiple myelomas with the t(4;14)(p16;q32) translocation and poor prognosis. With the aim of understanding, if MMSET can be involved...

  17. Surfing the web and parkinson's law.

    Science.gov (United States)

    Baldwin, F D

    1996-05-01

    The World Wide Web accounts for much of the popular interest in the Internet and offers a rich and variegated source of medical information. It's where you'll find online attractions ranging from "The Visible Human" to collections of lawyer jokes, as well as guides to clinical materials. Here's a basic introduction to the Web, its features, and its vocabulary.

  18. Flowering Trees

    Indian Academy of Sciences (India)

    stems and handsome foliage. Leaves are 8–10 cm long, dull green, the two thin leathery halves of the lamina fusing or the cleft between them extending beyond the middle. Flowers are gorgeous, axillary with dark purple stamens. The pod is more or less flat. B. alba is often named as B. variegate var. alba by botanists.

  19. 7 CFR 29.3532 - Mixed (M).

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 2 2010-01-01 2010-01-01 false Mixed (M). 29.3532 Section 29.3532 Agriculture Regulations of the Department of Agriculture AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing... Type 95) § 29.3532 Mixed (M). Variegated or distinctly different colors of the type mingled together...

  20. 7 CFR 29.2481 - Key to standard grademarks.

    Science.gov (United States)

    2010-01-01

    ... Agriculture Regulations of the Department of Agriculture AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE COMMODITY STANDARDS AND STANDARD CONTAINER.... 3—Good. 4—Fair. 5—Low. Colors L—Light brown. F—Medium brown. D—Dark brown. M—Mixed or variegated. G...

  1. 7 CFR 29.2696 - Key to standard grademarks.

    Science.gov (United States)

    2010-01-01

    ... Agriculture Regulations of the Department of Agriculture AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE COMMODITY STANDARDS AND STANDARD CONTAINER.... 3—Good. 4—Fair. 5—Low. Colors L—Light brown. F—Medium brown. D—Dark brown. M—Mixed or variegates. VF...

  2. Ford's Fund for the Republic: A 1950s-Era Foundation as Educator

    Science.gov (United States)

    Walton, Andrea

    2015-01-01

    Historians have recently opened up a reconsideration of the 1950s. Long characterized as a time of stolid conformity and Cold War conservatism, the era is increasingly seen in more variegated terms. Studies exploring a range of institutions, causes, and activities have illuminated ways the intellectual and social soil of postwar America gave root…

  3. Destruction of the Phoenix/Hibiscus and Barringtonia racemosa Communities at Richards Bay, Natal, South Africa

    Directory of Open Access Journals (Sweden)

    P. J. Weisser

    1982-10-01

    Full Text Available The destruction of the Phoenix!Hibiscus and Barringtonia racemosa Communities described by Venter in 1972 on the southern shores of Richards Bay is reported. The cause was the artificial openingof a new mouth about 5,5 km south of the original mouth, which increased tidal range and salinity. These swamp communities occupied a narrow band about 6 ha in area behind the Bruguiera gymnorrhiza Community. An estimated 95 % of the communities was affected and only on the landward border were some isolated remnants of species such as Acrostichum aureum, Hibiscus tiliaceus and Phoenix reclinata detected .Young stands of  Phragmites australis, seedlings of  Bruguiera gymnorrhiza and Avicennia marina and epipelic algae are recoIonizing the affected area.

  4. Rhizospheric metagenome of the terrestrial mangrove fern Acrostichum from Indian Sunderbans

    Directory of Open Access Journals (Sweden)

    Sayak Ganguli

    2017-12-01

    Full Text Available This study reports the analyses of the rhizospheric microbiome of the terrestrial mangrove fern Acrostichum aureum Linn. from the Indian Sunderbans. Samples were collected using standard protocols and 16S rRNA gene V3–V4 region amplicon sequencing was performed to identify the microbial communities prevalent in the rhizosphere. A total of 1,931,252 quality checked reads were assembled into 204,818 contigs and were analysed using QIIME to reveal the abundance of Proteobacteria, Acidobacteria and Planctomycetes. The data is available at the NCBI - Sequence Read Archive with accession number: SRX2660456. This is the first report of the rhizospheric microbiome belonging to a fern species.

  5. Common plant toxicology: A comparison of national and Southwest Ohio data trends on plant poisonings in the 21st century

    International Nuclear Information System (INIS)

    Petersen, Dan D.

    2011-01-01

    Data from the American Association of Poison Control Centers (AAPCC) and the Cincinnati-based Drug and Poison Information Center (DPIC) were analyzed to determine the incidence and trends of human plant poisonings since the year 2000. Approximately 3.4% of the approximately 4.3 million annual calls to the AAPCC centers involved plants, with a higher fraction (4.5%) for pediatric exposures. Nearly 70% of plant exposures occurred in children under six. Only 8% of cases required treatment in a health-care facility, and only 0.1% (in 2008) were considered severe outcomes. The most prominent groups of plants involved in exposures are those containing oxalates, and the most common symptom is gastroenteritis. The top 12 identified plants (in descending order) nationally were Spathiphyllum species (peace lilly), Philodendron species (philodendron), Euphorbia pulcherrima (poinssettia), Ilex species (holly), Phytolacca americana (pokeweed), Toxicodendron radicans (poison ivy), Capsicum (pepper), Ficus (rubber tree, weeping fig), Crassula argentea (jade plant), Diffenbachia (dumb cane), Epipremnum areum (pothos) and Schlumbergera bridesii (Christmas cactus). Broad overlaps between the DPIC and the AAPCC incidence data were noted, with essentially the same plant species in each dataset. The nature of the various toxins, the symptomatology and potential treatments are discussed for the highest ranking plant species.

  6. The effects of chromosome rearrangements on the expression of heterochromatic genes in chromosome 2L of Drosophila melanogaster

    International Nuclear Information System (INIS)

    Wakimoto, B.T.; Hearn, M.G.

    1990-01-01

    The light (lt) gene of Drosophila melanogaster is located at the base of the left arm of chromosome 2, within or very near centromeric heterochromatin (2Lh). Chromosome rearrangements that move the lt + gene from its normal proximal position and place the gene in distal euchromatin result in mosaic or variegated expression of the gene. The cytogenetic and genetic properties of 17 lt-variegated rearrangements induced by X radiation are described in this report. The authors show that five of the heterochromatic genes adjacent to lt are subject to inactivation by these rearrangements and that the euchromatic loci in proximal 2L are not detectably affected. The properties of the rearrangements suggest that proximity to heterochromatin is an important regulatory requirement for at least six 2Lh genes. They discuss how the properties of the position effects on heterochromatic genes relate to other proximity-dependent phenomena such as transvection

  7. Selected Jesus sayings on materialism according to the Sermon on the Mount (Matt. 5-7) in judging the so-called prosperity theology

    OpenAIRE

    2008-01-01

    Prosperity theology is a variegated movement that overlaps both the Charismatic and non-Charismatic spectrums. This theology certainly has a Charismatic flavouring, but it is by no means limited to Pentecostalism. The prosperity message is being preached world-wide on TBN Television, radio and printed media. This gospel focuses on human potential for successful living, emphasizing health and wealth. There is a clear shift notable from theocentric providence to anthropocentric prosperity in th...

  8. Toward an African-Christian Demonology: The Demonologies of African Traditional Religion, African Christianity, and Early Christianity in Dialogue

    OpenAIRE

    Moscicke, Hans

    2017-01-01

    Christian interactions with the spirit-world of African traditional religion (ATR) have remained problematic due, in part, to the failure of Christian missionaries to understand in detail the cosmology of ATR and the angelologies and demonologies of early Christianity. In this article I suggest a way beyond this impasse. I first survey the shape of cosmology and demonology in ATR, and then examine African Christianitys variegated responses to these traditional beliefs. Finally, I submit that ...

  9. Distribution of the Sunda Colugo (Galeopterus variegatus) in Malaysia (Peninsular, Sabah, Sarawak)

    OpenAIRE

    Nasir, Muhammad Dzulhelmi Muhammad; Abdullah, Mohd Tajuddin

    2010-01-01

    There is not much information available on the distribution of the Sunda colugo (Galeopterus variegates) in Malaysia, despite it being one of only two known species in the order Dermoptera. Data on the presence of the Sunda colugo and the vernacular names used by various ethnic groups throughout Malaysia were collected and compiled from various primary and secondary sources. There were 27 locations from Peninsular, 11 locations from Sabah and 34 locations from Sarawak that reported the presen...

  10. Listener: a probe into information based material specification

    DEFF Research Database (Denmark)

    Ramsgaard Thomsen, Mette; Karmon, Ayelet

    2011-01-01

    This paper presents the thinking and making of the architectural research probe Listener. Developed as an interdisciplinary collaboration between textile design and architecture, Listener explores how information based fabrication technologies are challenging the material practices of architecture....... The paper investigates how textile design can be understood as a model for architectural production providing new strategies for material specification and allowing the thinking of material as inherently variegated and performative. The paper traces the two fold information based strategies present...

  11. ACADIA 2010 konference: listener

    DEFF Research Database (Denmark)

    Ramsgaard Thomsen, Mette; Karmon, Ayelet

    2010-01-01

    This paper presents the thinking and making of the architectural research probe Listener. Developed as an interdisciplinary collaboration between textile design and architecture, Listener explores how information based fabrication technologies are challenging the material practices of architecture....... The paper investigates how textile design can be understood as a model for architectural production providing new strategies for material specification and allowing the thinking of material as inherently variegated and performative. The paper traces the two fold information based strategies present...

  12. Beyond bureaucracy and entrepreneurialism:examining the multiple discursive codes informing the work, careers and subjectivities of management graduates

    OpenAIRE

    Loacker, Bernadette Isabel; Sliwa, Martyna

    2016-01-01

    This paper examines how discursive codes and demands associated with ‘bureaucratic and entrepreneurial regimes’ of work and career organization shape the work, careers and subjectivities of management graduates. The study is based on an analysis of 30 narratives of management professionals who graduated from an Austrian business school in the early 1970s or 2000s. Its insights suggest that variegated discursive codes manifest in the graduates’ articulated professional practices and subjectivi...

  13. Analytical Approach to Fictional Elements of Sandbadnameh

    Directory of Open Access Journals (Sweden)

    a عارفی

    2014-05-01

    Using stock and allegorical characters, fixed tone and style in dialogue tales, lack of variegated plot and logical and stable relationship between events make this work far from contemporary criteria of fiction writing in spite of its great volume, and introduce it as a symbolic and allegorical tale. On the other hand, bombastic and embellished prose as well as being loyal to ancient fiction writing have added to literary significance of this literary work.

  14. 7 CFR 29.2509 - Color symbols.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 2 2010-01-01 2010-01-01 false Color symbols. 29.2509 Section 29.2509 Agriculture...-Cured Tobacco (u.s. Types 22, 23, and Foreign Type 96) § 29.2509 Color symbols. As applied to these types, color symbols are L—light brown, F—medium brown, D—dark brown, M—mixed or variegated VF—greenish...

  15. Margie Orford’s Hybrid Narratives: Crime Fiction Subgenres and the Theme of Misogyny in Daddy’s Girl and Like Clockwork

    OpenAIRE

    Horne, Karina Sofi

    2015-01-01

    This thesis sets out to identity the different types of subgenres that constitute Margie Orford’s crime fiction novels Like Clockwork and Daddy’s Girl, and to explore their connections to the extensive and variegated descriptions of misogyny that pervades both novels. The research consists of two parts: The first part provides a general description of the different subgenres that Orford’s works make use of: Classical detective fiction, hardboiled detective fiction, the police procedural/novel...

  16. [Mercury in three species of Suillus mushroom from some sites in Poland].

    Science.gov (United States)

    Mielewska, Dominika; Stefańska, Aleksandra; Wenta, Justyna; Mazur, Michalina; Bielawski, Leszek; Danisiewicz, Dorota; Dryzałowska, Anna; Falandysz, Jerzy

    2008-01-01

    Total mercury content have been determined in fruiting bodies of Variegated Bolete (Suillus variegates), European Cow Bolete (S. bovinus) and Slippery Jack (S. luteus) and in underlying to mushroom's surface layer of soil substrate collected from several spatially distant one from another sites in Poland. All three Suillus mushroom species independent of the site characterized were by small mercury content. The arithmetic mean values of mercury concentration in caps and stipes, respectively, were: 0.17 +/- 0.07-0.22 +/- 0.12 and 0.047 +/- 0.015-0.071 +/- 0.035 microg/g dry weight for Variegated Bolete; 0.28 +/- 0.11-0.79 +/- 0.40 and 0.17 +/- 0.07- 0.51 +/- 0.22 microg/g dw for European Cow Bolete, and 0.095 +/- 0.082-0.17 +/- 0.05 and 0.045 +/- 0.026- 0.070 +/- 0.026 microg/g dw for Slippery Jack. All three species of Suillus mushrooms bio-concentrated mercury (BCF > 1). European Cow Bolete bioconcentrated mercury relatively more efficiently when compared to two other species, and means of BCF value of this element in its caps ranged from 18 +/- 10 to 45 +/- 20, and in stipes from 9.4 +/- 7.5 to 29 +/- 11. A level of surface soil pollution with mercury was low and averaged from 0.017 +/- 0.003 do 0.029 +/- 0.020 microg/g dw.

  17. Chemical resistance of the gram-negative bacteria to different sanitizers in a water purification system

    Directory of Open Access Journals (Sweden)

    Penna Thereza CV

    2006-08-01

    Full Text Available Abstract Background Purified water for pharmaceutical purposes must be free of microbial contamination and pyrogens. Even with the additional sanitary and disinfecting treatments applied to the system (sequential operational stages, Pseudomonas aeruginosa, Pseudomonas fluorescens, Pseudomonas alcaligenes, Pseudomonas picketti, Flavobacterium aureum, Acinetobacter lowffi and Pseudomonas diminuta were isolated and identified from a thirteen-stage purification system. To evaluate the efficacy of the chemical agents used in the disinfecting process along with those used to adjust chemical characteristics of the system, over the identified bacteria, the kinetic parameter of killing time (D-value necessary to inactivate 90% of the initial bioburden (decimal reduction time was experimentally determined. Methods Pseudomonas aeruginosa, Pseudomonas fluorescens, Pseudomonas alcaligenes, Pseudomonas picketti, Flavobacterium aureum, Acinetobacter lowffi and Pseudomonas diminuta were called in house (wild bacteria. Pseudomonas diminuta ATCC 11568, Pseudomonas alcaligenes INCQS , Pseudomonas aeruginosa ATCC 15442, Pseudomonas fluorescens ATCC 3178, Pseudomonas picketti ATCC 5031, Bacillus subtilis ATCC 937 and Escherichia coli ATCC 25922 were used as 'standard' bacteria to evaluate resistance at 25°C against either 0.5% citric acid, 0.5% hydrochloric acid, 70% ethanol, 0.5% sodium bisulfite, 0.4% sodium hydroxide, 0.5% sodium hypochlorite, or a mixture of 2.2% hydrogen peroxide (H2O2 and 0.45% peracetic acid. Results The efficacy of the sanitizers varied with concentration and contact time to reduce decimal logarithmic (log10 population (n cycles. To kill 90% of the initial population (or one log10 cycle, the necessary time (D-value was for P. aeruginosa into: (i 0.5% citric acid, D = 3.8 min; (ii 0.5% hydrochloric acid, D = 6.9 min; (iii 70% ethanol, D = 9.7 min; (iv 0.5% sodium bisulfite, D = 5.3 min; (v 0.4% sodium hydroxide, D = 14.2 min; (vi 0.5% sodium

  18. Multiplexed lateral flow microarray assay for detection of citrus pathogens Xylella fastidiosa and Xanthomonas axonopodis pv citri

    Energy Technology Data Exchange (ETDEWEB)

    Cary,; Bruce, R [Santa Fe, NM; Stubben, Christopher J [Los Alamos, NM

    2011-03-22

    The invention provides highly sensitive and specific assays for the major citrus pathogens Xylella fastidiosa and Xanthomonas axonopodis, including a field deployable multiplexed assay capable of rapidly assaying for both pathogens simultaneously. The assays are directed at particular gene targets derived from pathogenic strains that specifically cause the major citrus diseases of citrus variegated chlorosis (Xylella fastidiosa 9a5c) and citrus canker (Xanthomonas axonopodis pv citri). The citrus pathogen assays of the invention offer femtomole sensitivity, excellent linear dynamic range, and rapid and specific detection.

  19. Crystal structure of a small heat-shock protein from Xylella fastidiosa reveals a distinct high-order structure.

    Science.gov (United States)

    Fonseca, Emanuella Maria Barreto; Scorsato, Valéria; Dos Santos, Marcelo Leite; Júnior, Atilio Tomazini; Tada, Susely Ferraz Siqueira; Dos Santos, Clelton Aparecido; de Toledo, Marcelo Augusto Szymanski; de Souza, Anete Pereira; Polikarpov, Igor; Aparicio, Ricardo

    2017-04-01

    Citrus variegated chlorosis is a disease that attacks economically important citrus plantations and is caused by the plant-pathogenic bacterium Xylella fastidiosa. In this work, the structure of a small heat-shock protein from X. fastidiosa (XfsHSP17.9) is reported. The high-order structures of small heat-shock proteins from other organisms are arranged in the forms of double-disc, hollow-sphere or spherical assemblies. Unexpectedly, the structure reported here reveals a high-order architecture forming a nearly square cavity.

  20. Toward an African-Christian Demonology: The Demonologies of African Traditional Religion, African Christianity, and Early Christianity in Dialogue

    Directory of Open Access Journals (Sweden)

    Moscicke, Hans

    2017-11-01

    Full Text Available Christian interactions with the spirit-world of African traditional religion (ATR have remained problematic due, in part, to the failure of Christian missionaries to understand in detail the cosmology of ATR and the angelologies and demonologies of early Christianity. In this article I suggest a way beyond this impasse. I first survey the shape of cosmology and demonology in ATR, and then examine African Christianitys variegated responses to these traditional beliefs. Finally, I submit that a retrieval of the early Christian mythological narrative about demons and its associated cosmology could help theologians accommodate the spirit-world of ATR within a truly African-Christian theology.

  1. Overview of technical trend of optical fiber/cable and research and development strategy of Samsung

    Science.gov (United States)

    Kim, Jin H.

    2005-01-01

    Fiber-to-the-Premise (FTTP), a keyword in the current fiber and cable industry, leads us variegated directions of the research and development activities. In fact, this momentum of industry seems to be weak yet, since the bandwidth demand by market is still unbalanced to the capacity in the several market segments. However, the recent gradual recovery in metro and access network indicates a positive sign for FTTP deployment projects. It is the very preferable for us to optimize R&D strategy applicable to the current market trend of sequential investment.

  2. Datation U_Pb : âge de mise en place du magmatisme bimodal des Jebilet centrales (chaîne Varisque, Maroc). Implications géodynamiquesU_Pb dating: emplacement age of the bimodal magmatism of central Jebilet (Variscan Belt, Morocco). Geodynamic implications

    Science.gov (United States)

    Essaifi, Abderrahim; Potrel, Alain; Capdevila, Ramon; Lagarde, Jean-Louis

    2003-01-01

    The bimodal magmatism of central Jebilet is dated to 330.5 +0.68-0.83 Ma by UPb dating on zircons. This age, similar to that of the syntectonic Jebilet cordierite-bearing granitoids, corresponds to the age of the local major tectonometamorphic event. The syntectonic plutonism of the Jebilet massif, composed of tholeiitic, alkaline, and peraluminous calc-alkaline series, is variegated. Magmas emplacement was favoured by the local extension induced by the motion along the western boundary of the Carboniferous basins of the Moroccan Meseta. The Jebilet massif exemplifies the activation of various magmas sources during an episode of continental convergence and crustal wrenching.

  3. Constructing Confucius in the Low Countries

    Directory of Open Access Journals (Sweden)

    Trude Dijkstra

    2017-02-01

    Full Text Available The first translation of Confucius’s Analects into a European language was a Dutch book by Pieter van Hoorn. Printed in Batavia in 1675, it predated the better-known Latin translation, Confucius Sinarum Philosophus (1687. Whereas the introduction of Confucius in the West has often been regarded as a project of the Jesuit mission, an exploration of the Netherlandish situation points out that the ‘manufacturing’ of Confucianism was a variegated and multi-confessional affair. The process of transmitting, translating, publishing, explaining, and judging Confucius presented a challenge to Europeans from different backgrounds and allegiances, integrating not only Latin and vernacular scholarship but also Asian expertise.

  4. Phytochemical and Biological investigations of Erythrina Variegata

    International Nuclear Information System (INIS)

    Rahman, Mohammad Z.; Sultana, Shirin J.; Islam, Mohammad S.; Faruquee, Chowdhury F.; Ferdous, F.; Rahman, Mohammad S.; Rashid, Mohammad A.

    2007-01-01

    Five compounds were isolated from the n-hexane and chloroform soluble fractions of a methanol extract of the stem bark of Erythrina variegate. The structure of the isolated compounds were elucidated as alpinum isoflavone (1), epilupeol (2), 6-hydroxygenestein (3) 3-beta, 28-dihydroxylolean-12-ene (4) and stigmasterol (5) by extensive spectroscopic studies including high field NMR analyses. Different partitionates of the methanol extract exhibited mild to moderate antimicrobial activity and varying degrees of cytotoxicity. Although, alpinum isoflavone (1) has previously been reported from E. Variegata, this is the first report of isolation of compounds 2-4 and stigmasterol from this plant species. (author)

  5. Anisian and Ladinian Beds in the Cross-Section above Srednik Valley at Križna Gora,Central Slovenia

    Directory of Open Access Journals (Sweden)

    Matevž Demšar

    2003-06-01

    Full Text Available The Križna Gora and wider surroundings are built of the Paleozoic and Mesozoic rocks. Pretty extended among them are the carbonate Anisian and heterogeneously developed variegated Ladinian rocks wedging out laterally and vertically already at short distances as a result of active volcanism, tectonics and the fact that this region is situated on the margin between the Dinaric Carbonate Platform and the Slovenian Basin. The Ladinian rocks lie discordantly upon the Anisian dolomite. In the Ladinian sediments pelecypods Daonella lommeli (Wissmann are rather extended. The Ladinian beds are overlain by theCordevolian dolomite. The boundary between them in the Srednik Valley is strongly tektonized.

  6. Cytological study of radiation induced alterations in cytoplasmic factors controlling male sterility in corn. Progress report, February 28, 1975--December 1, 1975

    International Nuclear Information System (INIS)

    Edwardson, J.R.

    1975-01-01

    Progress is reported on the following research projects: cytoplasmic constituents of the embryo of various gymnosperms and angiosperms; cytoplasmic male sterility in corn; modification of cytoplasmic sterility factors using gamma radiation, EMS, and ethidium bromide; selection for sterile, blight-resistant corn plants; electron microscopy study of abnormal mitochondria in cytoplasm of corn; cytoplasmic male sterility in Petunia; non-Mendelian variegation in Petunia and Nicotiana; graft transmission of cytoplasmic male sterility; cytoplasmic male sterility in Vicia faba; and studies on Blakeslee's I virus in Datura

  7. The DCL gene of tomato is required for chloroplast development and palisade cell morphogenesis in leaves.

    OpenAIRE

    Keddie, J S; Carroll, B; Jones, J D; Gruissem, W

    1996-01-01

    The defective chloroplasts and leaves-mutable (dcl-m) mutation of tomato was identified in a Ds mutagenesis screen. This unstable mutation affects both chloroplast development and palisade cell morphogenesis in leaves. Mutant plants are clonally variegated as a result of somatic excision of Ds and have albino leaves with green sectors. Leaf midribs and stems are light green with sectors of dark green tissue but fruit and petals are wild-type in appearance. Within dark green sectors of dcl-m l...

  8. Reconstructing Historical Light Curves of Symbiotic Stars and Novae

    Directory of Open Access Journals (Sweden)

    Jurdana-Šepić R.

    2012-06-01

    Full Text Available We reconstructed photometric histories of symbiotic stars and novae from direct inspection and measurement of photographic plates preserved at historical archives. We have completed the digging of the rich Asiago archive, and have started working on the Harvard plate stack, while other plate collections should be added soon. For homogeneity, we use the same UBV RCIC photometric comparison sequences used in current CCD observations. This data harvest has permitted the discovery of past undetected outbursts and secular trends, or to derive previously unknown orbital periods and recurrence times, which are essential to constrain the nature of these capricious and variegated active binaries.

  9. Elemental composition changes in citrus affected by the CVC disease

    International Nuclear Information System (INIS)

    Nadai Fernandes de, A.A.; Tagliaferro, F.S.; Turra, C.; Franca de, E.J.; Bacchi, M.A.

    2008-01-01

    The citrus variegated chlorosis (CVC) disease results in serious economical losses for the Brazilian citriculture. The influence of CVC disease on the elemental composition of citrus plants was investigated. Leaves of sweet orange varieties Hamlin, Pera Rio and Valencia were collected from healthy and CVC-affected trees for chemical characterization by instrumental neutron activation analysis (INAA). Significant differences between healthy and CVC-affected leaves were identified for Ca, Ce, Co, Eu, Fe, K, La, Na, Nd, Rb, Sc and Sm. Rare earth elements presented consistently higher mass fractions in the healthy leaves. (author)

  10. Echocardiographic study of left atrial myxoma

    Directory of Open Access Journals (Sweden)

    Dalal J

    1979-01-01

    Full Text Available Four cases of left atrial myxoma were diagnosed pre-operatively by echocardiography. All cases showed characteristic echocardio-graphic features of variegated shadows behind the mitral valve in diastole and within the left atrium in systole. In two cases the my-xomas were surgically removed and confirmed on histology. In one case the post-operative echocardiogram showed complete dis-appearance of the abnormal shadows. Echocardiography is the most reliable method today for the diagnosis of a myxoma.

  11. Trophic Habits, Morphometry and Gonadal Status of Five Fish Species in Different Climatic Periods at the Rio Sogamoso (Santander, Colombia)

    International Nuclear Information System (INIS)

    Ramirez C, Angelica; Pinilla A, Gabriel

    2012-01-01

    The objective of this research was to determine some bioecological aspects for five fish species of the Rio Sogamoso, to generate baseline information that will permit analyze the impacts generated for the construction and operation of the Hidrosogamoso dam. For each specimen collected the standard length and total weight were recorded, the correlation between weight and length (W= aLb) was calculated, the gonadal status and the coefficient of vacuity were determined, and the importance of each food category was defined. The Bocachico (Prochilodus magdalenae) reached greatest abundance in the river during the dry season and the beginning of spawning was recorded in December. Its vacuity coefficient was high and its diet was based on organic matter. Pimelodus blochii and P. grosskopfii reported the greatest catches in December; their diet was based on insects and fish. The Loricariids Chaetostoma cf. thomsoni and Sturisoma aureum were captured only in the middle and upper reaches of river. Their diet was based on organic matter. It is required a more detailed and continue study to set the reproductive cycle of Pimelodus spp. and Loricariids. Given the status of threatened species and its migration dynamics, the Bocachico is one of the most sensitive species to the construction, filling and operation of the hydroelectric dam on the Rio Sogamoso. For Loricariids, the reduction of Rio Sogamoso flow may decrease their habitats and limit the connection to the tributaries through which they migrate.

  12. Cytotoxic Effects of Bangladeshi Medicinal Plant Extracts

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    Shaikh J. Uddin

    2011-01-01

    Full Text Available To investigate the cytotoxic effect of some Bangladeshi medicinal plant extracts, 16 Bangladeshi medicinal plants were successively extracted with n-hexane, dichloromethane, methanol and water. The methanolic and aqueous extracts were screened for cytotoxic activity against healthy mouse fibroblasts (NIH3T3 and three human cancer-cell lines (gastric: AGS; colon: HT-29; and breast: MDA-MB-435S using the MTT assay. Two methanolic extracts (Hygrophila auriculata and Hibiscus tiliaceous and one aqueous extract (Limnophila indica showed no toxicity against healthy mouse fibroblasts, but selective cytotoxicity against breast cancer cells (IC50 1.1–1.6 mg mL−1. Seven methanolic extracts from L. indica, Clerodendron inerme, Cynometra ramiflora, Xylocarpus moluccensis, Argemone mexicana, Ammannia baccifera and Acrostichum aureum and four aqueous extracts from Hygrophila auriculata, Bruguiera gymnorrhiza, X. moluccensis and Aegiceras corniculatum showed low toxicity (IC50 > 2.5 mg mL−1 against mouse fibroblasts but selective cytotoxicity (IC50 0.2–2.3 mg mL−1 against different cancer cell lines. The methanolic extract of Blumea lacera showed the highest cytotoxicity (IC50 0.01–0.08 mg mL−1 against all tested cell lines among all extracts tested in this study. For some of the plants their traditional use as anticancer treatments correlates with the cytotoxic results, whereas for others so far unknown cytotoxic activities were identified.

  13. Chromosome mapping of repetitive sequences in four Serrasalmidae species (Characiformes

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    Leila Braga Ribeiro

    2014-01-01

    Full Text Available The Serrasalmidae family is composed of a number of commercially interesting species, mainly in the Amazon region where most of these fishes occur. In the present study, we investigated the genomic organization of the 18S and 5S rDNA and telomeric sequences in mitotic chromosomes of four species from the basal clade of the Serrasalmidae family: Colossoma macropomum, Mylossoma aureum, M. duriventre, and Piaractus mesopotamicus, in order to understand the chromosomal evolution in the family. All the species studied had diploid numbers 2n = 54 and exclusively biarmed chromosomes, but variations of the karyotypic formulas were observed. C-banding resulted in similar patterns among the analyzed species, with heterochromatic blocks mainly present in centromeric regions. The 18S rDNA mapping of C. macropomum and P. mesopotamicus revealed multiple sites of this gene; 5S rDNA sites were detected in two chromosome pairs in all species, although not all of them were homeologs. Hybridization with a telomeric probe revealed signals in the terminal portions of chromosomes in all the species and an interstitial signal was observed in one pair of C. macropomum.

  14. Vegetation Detection in Stress of Moisture Shortage Based on Laser Speckle Recognition

    Science.gov (United States)

    Ishizawa, Hiroaki; Matsuo, Tsukasa; Miki, Takashi

    This paper describes a new measuring method of plant vigor by using Laser speckle pattern. Furthermore, this proposes a practical application of this presented measurement system. The measuring instrument is consisted by a He-Ne Laser as the light source, and a set of optics, such as reflectors, a beam expander. The speckle pattern could be measured by a CCD camera through lenses. A Pothos (Epiremnum aureum) and Japanese morning glory (Ipomoea nil) were used as the sample plant. Their intact leaves were measured the speckle pattern images. Visible but small vigor veins could be clearly observed in the images obtained by the speckle patterns. On the other hand, withered ones have shown different images. The relationship has been obtained between the feature of the images and the chlorophyll degradation. It would be expected that the symptom of plant against some stress could be detected by measuring the Laser speckle pattern. It could be used as the sensor of the field server system at every field monitoring site.

  15. Mangrove associated lignite beds of Malvan, Konkan: Evidence for higher sea-level during the Late Tertiary (Neogene) along the west coast of India

    Energy Technology Data Exchange (ETDEWEB)

    Kumaran, K.P.N.; Shindikar, M.; Limaye, R.B. [Agharkar Research Inst., Pune (India)

    2004-01-25

    Fossil pneumatophores (breathing roots) of Avicennia are recovered and reported from the lignite beds exposed in Kolamb well-section near Malvan, Konkan area of western Maharashtra. The accrued palynoflora is dominated by mangroves (Avicennia, Aegialitis, Excoecaria, Rhizophora and Sonneratia). The spores of mangrove fern (Acrostichum aureum) an estuarine fungus Cirrenalia indicate that these lignites are autochthonous and deposited in a near-shore environment. Presence of foraminiferal linings (= microforaminifera), dinoflagellate cysts, a few calcareous nannofossils and scolecodonts is an irrefutable proof of marine and brackish water influence during the deposition of lignites under intertidal/tidal swampy condition (mangrove influenced) with fair input from freshwater swamps and hinterland. Freshwater-related forms, viz. Ceratopteris thalictroides, Nymphaeaceae, Ctenolophonaceae and hinterland taxa (Cullenia/Durio) of Bombacaceae along with abundance of microthyriaceous fungi in the palynoflora imply a warm humid tropical climate with high precipitation during the depositional period. The presence of Ctenolophon englerianus (= Ctenolophonidites costatus) in Kolamb lignites suggests the Late Neogene (Late Miocene-Early Pliocene) age. The occurrence of pneumatophores and associated lignite deposits about 37 m above the present mean sea-level, and much inland, clearly indicates the higher sea-level strand during Late Neogene along the west coast of India.

  16. Investigating the Potential of Using the Spatial and Spectral Information of Multispectral LiDAR for Object Classification

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    Wei Gong

    2015-09-01

    Full Text Available The abilities of multispectral LiDAR (MSL as a new high-potential active instrument for remote sensing have not been fully revealed. This study demonstrates the potential of using the spectral and spatial features derived from a novel MSL to discriminate surface objects. Data acquired with the MSL include distance information and the intensities of four wavelengths at 556, 670, 700, and 780 nm channels. A support vector machine was used to classify diverse objects in the experimental scene into seven types: wall, ceramic pots, Cactaceae, carton, plastic foam block, and healthy and dead leaves of E. aureum. Different features were used during classification to compare the performance of different detection systems. The spectral backscattered reflectance of one wavelength and distance represented the features from an equivalent single-wavelength LiDAR system; reflectance of the four wavelengths represented the features from an equivalent multispectral image with four bands. Results showed that the overall accuracy of using MSL data was as high as 88.7%, this value was 9.8%–39.2% higher than those obtained using a single-wavelength LiDAR, and 4.2% higher than for multispectral image.

  17. Il mare della fertilità Una analisi antropologica della tetralogia di Mishima Yukio

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    Giovanni Azzaroni

    2013-05-01

    Full Text Available Abstract – IT Neve di primavera è il primo romanzo della tetralogia 'Il mare della fertilità', il capolavoro dello scrittore giapponese, che si snoda in un Giappone caratterizzato sia da una cultura ancestrale sia da una invadente modernità e prelude a quel drammatico 25 novembre 1970 quando Mishima, scritte le ultime parole della tetralogia, si suicidò con l’antico rituale del seppuku. Neve di primavera è una narrazione polisemica costruita su opposizioni binarie strutturali, le vicende dei personaggi sono calate in un preciso contesto storico e culturale e per decodificarle è proposta una metodologia antropologica, nel tentativo, al tempo stesso, di coglierne le variegate e molteplici sfumature e, nel contempo, anche le interconnessioni con il tessuto ontologico che ne ha favorito il nascere e lo svilupparsi. Abstract – EN Spring snow is the first novel of the tetralogy The sea of Fertility, the masterpiece of the Japanese writer, which takes place in ancient and modern Japan. The novel is a prelude to the dramatic 25th November 1970: Mishima finished the tetralogy and committed suicide by the ancient seppuku ritual. Spring snow is a polisemic story which is constructed by binary and structural oppositions, the plot of the characters is drawn on a exact, historical and cultural context to catch either the variegated and various shadings or the relations with the ontological substratum, which helped its birth and development.

  18. Il mare della fertilità Una analisi antropologica della tetralogia di Mishima Yukio

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    Giovanni Azzaroni

    2012-01-01

    Full Text Available Abstract – IT Neve di primavera è il primo romanzo della tetralogia Il mare della fertilità, il capolavoro dello scrittore giapponese, che si snoda in un Giappone caratterizzato sia da una cultura ancestrale sia da una invadente modernità e prelude a quel drammatico 25 novembre 1970 quando Mishima, scritte le ultime parole della tetralogia, si suicidò con l’antico rituale del seppuku. Neve di primavera è una narrazione polisemica costruita su opposizioni binarie strutturali, le vicende dei personaggi sono calate in un preciso contesto storico e culturale e per decodificarle è proposta una metodologia antropologica, nel tentativo, al tempo stesso, di coglierne le variegate e molteplici sfumature e, nel contempo, anche le interconnessioni con il tessuto ontologico che ne ha favorito il nascere e lo svilupparsi. Abstract – EN Spring snow is the first novel of the tetralogy The sea of Fertility, the masterpiece of the Japanese writer, which takes place in ancient and modern Japan. The novel is a prelude to the dramatic 25th November 1970: Mishima finished the tetralogy and committed suicide by the ancient seppuku ritual. Spring snow is a polisemic story which is constructed by binary and structural oppositions, the plot of the characters is drawn on a exact, historical and cultural context to catch either the variegated and various shadings or the relations with the ontological substratum, which helped its birth and development.

  19. The molecular basis for stability of heterochromatin-mediated silencing in mammals

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    Hiragami-Hamada Kyoko

    2009-11-01

    Full Text Available Abstract The archetypal epigenetic phenomenon of position effect variegation (PEV in Drosophila occurs when a gene is brought abnormally close to heterochromatin, resulting in stochastic silencing of the affected gene in a proportion of cells that would normally express it. PEV has been instrumental in unraveling epigenetic mechanisms. Using an in vivo mammalian model for PEV we have extensively investigated the molecular basis for heterochromatin-mediated gene silencing. Here we distinguish 'epigenetic effects' from other cellular differences by studying ex vivo cells that are identical, apart from the expression of the variegating gene which is silenced in a proportion of the cells. By separating cells according to transgene expression we show here that silencing appears to be associated with histone H3 lysine 9 trimethylation (H3K9me3, DNA methylation and the localization of the silenced gene to a specific nuclear compartment enriched in these modifications. In contrast, histone H3 acetylation (H3Ac and lysine 4 di or tri methylation (H3K4me2/3 are the predominant modifications associated with expression where we see the gene in a euchromatic compartment. Interestingly, DNA methylation and inaccessibility, rather than H3K9me3, correlated most strongly with resistance to de-repression by cellular activation. These results have important implications for understanding the contribution of specific factors involved in the establishment and maintenance of gene silencing and activation in vivo.

  20. Physiological differences and changes in global DNA methylation levels in Agave angustifolia Haw. albino variant somaclones during the micropropagation process.

    Science.gov (United States)

    Duarte-Aké, Fátima; Castillo-Castro, Eduardo; Pool, Felipe Barredo; Espadas, Francisco; Santamaría, Jorge M; Robert, Manuel L; De-la-Peña, Clelia

    2016-12-01

    Global DNA methylation changes caused by in vitro conditions are associated with the subculturing and phenotypic variation in Agave angustifolia Haw. While the relationship between the development of albinism and in vitro culture is well documented, the role of epigenetic processes in this development leaves some important questions unanswered. During the micropropagation of Agave angustifolia Haw., we found three different phenotypes, green (G), variegated (V) and albino (A). To understand the physiological and epigenetic differences among the somaclones, we analyzed several morphophysiological parameters and changes in the DNA methylation patterns in the three phenotypes during their in vitro development. We found that under in vitro conditions, the V plantlets maintained their CAM photosynthetic capacity, while the A variant showed no pigments and lost its CAM photosynthetic ability. Epigenetic analysis revealed that global DNA methylation increased in the G phenotype during the first two subcultures. However, after that time, DNA methylation levels declined. This hypomethylation correlated with the appearance of V shoots in the G plantlets. A similar correlation occurred in the V phenotype, where an increase of 2 % in the global DNA methylation levels was correlated with the generation of A shoots in the V plantlets. This suggests that an "epigenetic stress memory" during in vitro conditions causes a chromatin shift that favors the generation of variegated and albino shoots.

  1. The molecular basis for stability of heterochromatin-mediated silencing in mammals.

    Science.gov (United States)

    Hiragami-Hamada, Kyoko; Xie, Sheila Q; Saveliev, Alexander; Uribe-Lewis, Santiago; Pombo, Ana; Festenstein, Richard

    2009-11-04

    The archetypal epigenetic phenomenon of position effect variegation (PEV) in Drosophila occurs when a gene is brought abnormally close to heterochromatin, resulting in stochastic silencing of the affected gene in a proportion of cells that would normally express it. PEV has been instrumental in unraveling epigenetic mechanisms. Using an in vivo mammalian model for PEV we have extensively investigated the molecular basis for heterochromatin-mediated gene silencing. Here we distinguish 'epigenetic effects' from other cellular differences by studying ex vivo cells that are identical, apart from the expression of the variegating gene which is silenced in a proportion of the cells. By separating cells according to transgene expression we show here that silencing appears to be associated with histone H3 lysine 9 trimethylation (H3K9me3), DNA methylation and the localization of the silenced gene to a specific nuclear compartment enriched in these modifications. In contrast, histone H3 acetylation (H3Ac) and lysine 4 di or tri methylation (H3K4me2/3) are the predominant modifications associated with expression where we see the gene in a euchromatic compartment. Interestingly, DNA methylation and inaccessibility, rather than H3K9me3, correlated most strongly with resistance to de-repression by cellular activation. These results have important implications for understanding the contribution of specific factors involved in the establishment and maintenance of gene silencing and activation in vivo.

  2. A versatile optical profilometer based on conoscopic holography sensors for acquisition of specular and diffusive surfaces in artworks

    Science.gov (United States)

    Gaburro, Nicola; Marchioro, Giacomo; Daffara, Claudia

    2017-07-01

    Surface metrology of artworks requires the design of suitable devices for in-situ non-destructive measurement together with reliable procedures for an effective analysis of such non-engineered variegate objects. To advance the state-of-the-art it has been implemented a versatile optical micro-profilometry taking advantage of the adapt- ability of conoscopic holography sensors, able to operate with irregular shapes and composite materials (diffusive, specular, and polychrome) of artworks. The scanning technique is used to obtain wide field and high spatially resolved areal profilometry. The prototype has a modular scheme based on a set of conoscopic sensors, extending the typical design based on a scanning stage and a single probe with a limited bandwidth, thus allowing the collection of heights data from surface with different scales and materials with variegate optical response. The system was optimized by characterizing the quality of the measurement with the probes triggered in continuous scanning modality. The results obtained on examples of cultural heritage objects (2D paintings, 3D height-relief) and materials (pictorial, metallic) demonstrate the versatility of the implemented device.

  3. Mutations in the Arabidopsis AtMRS2-11/AtMGT10/VAR5 Gene Cause Leaf Reticulation

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    Shuang Liang

    2017-11-01

    Full Text Available In higher plants, the development of functional chloroplasts is essential for photosynthesis and many other physiological processes. With a long-term goal of elucidating the genetic regulation of chloroplast development, we identified two allelic leaf variegation mutants, variegated5-1 (var5-1 and var5-2. Both mutants showed a distinct leaf reticulation phenotype of yellow paraveinal regions and green interveinal regions, and the leaf reticulation phenotype correlated with photosynthetic defects. Through the identification of mutation sites in the two mutant alleles and the molecular complementation, we confirmed that VAR5 encodes a CorA family of Mg2+ transporters also known as AtMRS2-11/AtMGT10. Using protoplast transient expression and biochemical fractionation assays, we demonstrated that AtMRS2-11/AtMGT10/VAR5 likely localizes to the chloroplast envelope. Moreover, we established that AtMRS2-11/AtMGT10/VAR5 forms large molecular weight complexes in the chloroplast and the sizes of these complexes clearly exceed those of their bacterial counterparts, suggesting the compositions of CorA Mg2+ transporter complex is different between the chloroplast and bacteria. Our findings indicate that AtMRS2-11/AtMGT10/VAR5 plays an important role in the tissue specific regulation of chloroplast development.

  4. Induced chlorophyll variation in pineapple c v. 'queen' by gamma irradiation (60Cobalt)

    International Nuclear Information System (INIS)

    Valencia, Lolita DC.

    2012-01-01

    Irradiation using gamma rays ( 60 Cobalt) coupled with in vitro culture techniques was undertaken to induce variation or mutation in pineapple. Calli from crown meristem tips of pineapple cv 'Queen' [Ananas comosus (L.) Merr.] were initiated using Murashiege and Skoog's basal meduim supplemented with 10mg/liter 4-amino-3,5,6-trichloro-2-pyrodinecarboxylic acid (Picloram). High percentage of growth and shoot proliferation was observed in basal medium supplemented with 50 μM benzene adenine purine (BAP) and 10 μM gibberrelic acid (GA) after 8 weeks in vitro. Regenerants derived from shoots using different doses of gamma rays (0, 5, 10, 15, 20, 25 and 30 Gy) were evaluated under laboratory conditions. Of the different doses, 15 Gy produced the most variegation in young shoots (chlorophyll variants) maintained in vitro. The variants appeared to have yellow and green color combinations of the young leaves of pineapple 'Queen' variety. When transplanted inside the greenhouse, high percentage of plantlets survival was observed, ranging from 90-95%. Of the different irradiation doses, variegation in young leaves was observed at 15, 20, 25 and 30 Gy. Further assessment on the effect of irradiation is currently being unertaken under screen house conditions. The variants produced could serve a basis for selection of ornamental-type pineapple. In addition, protocols developed on the use of in vitro culture techniques could be utilized as a tool for induced mutation breeding in pineapple. (author)

  5. Chromatic illumination discrimination ability reveals that human colour constancy is optimised for blue daylight illuminations.

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    Bradley Pearce

    Full Text Available The phenomenon of colour constancy in human visual perception keeps surface colours constant, despite changes in their reflected light due to changing illumination. Although colour constancy has evolved under a constrained subset of illuminations, it is unknown whether its underlying mechanisms, thought to involve multiple components from retina to cortex, are optimised for particular environmental variations. Here we demonstrate a new method for investigating colour constancy using illumination matching in real scenes which, unlike previous methods using surface matching and simulated scenes, allows testing of multiple, real illuminations. We use real scenes consisting of solid familiar or unfamiliar objects against uniform or variegated backgrounds and compare discrimination performance for typical illuminations from the daylight chromaticity locus (approximately blue-yellow and atypical spectra from an orthogonal locus (approximately red-green, at correlated colour temperature 6700 K, all produced in real time by a 10-channel LED illuminator. We find that discrimination of illumination changes is poorer along the daylight locus than the atypical locus, and is poorest particularly for bluer illumination changes, demonstrating conversely that surface colour constancy is best for blue daylight illuminations. Illumination discrimination is also enhanced, and therefore colour constancy diminished, for uniform backgrounds, irrespective of the object type. These results are not explained by statistical properties of the scene signal changes at the retinal level. We conclude that high-level mechanisms of colour constancy are biased for the blue daylight illuminations and variegated backgrounds to which the human visual system has typically been exposed.

  6. WHITE PANICLE3, a Novel Nucleus-Encoded Mitochondrial Protein, Is Essential for Proper Development and Maintenance of Chloroplasts and Mitochondria in Rice

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    Hongchang Li

    2018-06-01

    Full Text Available Mitochondria and chloroplasts are interacting organelles that play important roles in plant development. In addition to a small number proteins encoded by their own genomes, the majority of mitochondrial and chloroplast proteins are encoded in the cell nucleus and imported into the organelle. As a consequence, coordination between mitochondria, chloroplasts, and the nucleus is of crucial importance to plant cells. Variegated mutants are chloroplast-defective mutants and are considered to be ideal models for studying the intercommunication between these organelles. Here, we report the isolation of WHITE PANICLE3 (WP3, a nuclear gene involved in variegation, from a naturally occurring white panicle rice mutant. Disrupted expression of WP3 in the mutant leads to severe developmental defects in both chloroplasts and mitochondria, and consequently causes the appearance of white-striped leaves and white panicles in the mutant plants. Further investigation showed that WP3 encodes a protein most likely targeted to mitochondria and is specifically expressed in rice panicles. Interestingly, we demonstrate that the recessive white-panicle phenotype in the wp3 mutant is inherited in a typical Mendelian manner, while the white-striped leaf phenotype in wp3 is maternally inherited. Our data collectively suggest that the nucleus-encoded mitochondrial protein, WP3, plays an essential role in the regulation of chloroplast development in rice panicles by maintaining functional mitochondria. Therefore, the wp3 mutant is an excellent model in which to explore the communication between the nucleus, mitochondria, and chloroplasts in plant cells.

  7. Myxomycetes do Parque Zoobotânico de Teresina, Piauí, Brasil Myxomycetes of the Parque Zoobotânico - Teresina, Piauí, Brazil

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    Márcia Percília Moura Parente Ponte

    2003-03-01

    Full Text Available Visando ampliar o conhecimento sobre a mixobiota do Piauí, foram realizadas coletas no Parque Zoobotânico de Teresina (05º 05' 12'' S, 42º 48' 48'' WG. A vegetação do Parque caracteriza-se como um cerradão com representantes da mata de cocais, que oferecem substratos potencialmente favoráveis ao desenvolvimento de Myxomycetes. Esporocarpos foram coletados no período chuvoso e início da estiagem (março a junho de 1999, identificados e depositados no herbário UFP (Universidade Federal de Pernambuco. Foram obtidas 101 amostras, correspondentes a 19 espécies, distribuídas nas famílias Ceratiomyxaceae (Ceratiomyxa fruticulosa (Mull. Macbr., Clastodermataceae (Clastoderma debaryanum Blytt, Cribrariaceae (Cribraria microcarpa (Schrad. Pers., C. violacea Rex, Dictydium canceilatum* (Batsch. Macbr., Enteridiaceae (Lycogala exiguum* Morgan., Physaraceae (Craterium aureum* (Schum. Rost., Physarum bogoriense* Racib., P. melleum (Berk. & Br. Massee, P. nucleatum Rex, P. pusilium* (Berk. & Curt. G. Lister, Trichiaceae (Arcyria denudata (L. Wett., A. cinerea (Bull. Pers., Hemitrichia calyculata (Speg. Farr, H. serpula (Scop. Rost., Metatrichia vesparium (Batsch Nann.-Brem., Perichaena depressa Lib. e Stemonitaceae (Comatricha typhoides (Bull. Rost., Stemonitis smithii Macbr.. Exceto H. calyculata e H. serpula, todos os táxons são referidos pela primeira vez para o município de Teresina; as espécies assinaladas com um asterisco constituem novos registros para o Piauí.Aiming to increase the knowledge about the myxobiota of Piauí State, Brazil, collections were done in the undertaken Zoobotanical Park of Teresina (05° 05' 12'' S, 42° 48' 48'' WG. The vegetation of the Park is a savanna ("cerradão" with "cocais" forest representative, offering substrates potentially favorable to the development of Myxomycetes. Sporocarps were collected in the rainy season (March to June 1999, identified and deposited at the UFP herbarium (Federal University

  8. Diversidade Estrutural de Bosques de Mangue e sua Relação com Distúrbios de Origem Antrópica: O Caso da Baía de Guanabara (Rio de Janeiro

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    Mário Luiz Gomes Soares

    2003-01-01

    Full Text Available Structural and functional characteristics of mangrove forests are primarily governedby the interaction of environmental characteristics, that act in global, regional or local 102Anuário do Instituto de Geociências - UFRJ Volume 26 / 2003scales. However, in areas submitted to strong influence of human activities, disturbanceshould be considered seriously. In this way, the structural characteristics of mangroves ofGuanabara bay were studied during three years (2000-2002. The studied forests presenteda high structural diversity, reflecting the action of disturbances, that determine differentdegradation levels and regeneration status (different phases of secondary succession of thestands. The stems density varied from zero (gaps to 52,800 stems.ha-1. The mean diameterat breast height varied from 1.33 cm to 7.83 cm and the medium tree height varied from1.80 m to 7.27 m. Several structural and dynamics patterns of the studied plants communitieswere identified, as follow: (a reverse " j " shaped, (b "normal" distribution, (c massmortality, (d overlap of "events", (e recolonization/gaps, (f regeneration failure, (ggaps and, (h invasion by Acrostichum aureum. The high structural diversity observed inthe studied forests, identified through the use of phytosociological parameters and speciescomposition, which determines a structural mosaic, is due to the overlapping of the followingcharacteristics: (1 variable nature of disturbances, (2 disturbances characteristics (chronicor acute, (3 spatial variability in disturbances action, (4 temporal variability in disturbancesaction, (5 variation in the frequency of disturbances occurrence and (6 capacity of thesystem to recover after a disturbance.

  9. INVENTARISASI KEANEKARAGAMAN ANGGREK (Orchidaceae DI HUTAN RESORT WAY KANAN BALAI AMAN NASIONAL WAY KAMBAS SEBAGAI SUMBER INFORMASI DALAM MELESTARIKAN PLASMA NUTFAH

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    Dwi Agustin Hening Widowati

    2015-10-01

    Full Text Available Abstract:  Orchid is one of the plants that have different characteristics in each species and its habitat. Orchids (Orchidaceae or nation is a group that is protected by Government Regulations  Number. 7 Th.1999. It is necessary to look at the type of data collections and save it, and look at the characteristics of tropical forests in the Resort Way Kanan. This research aims to inventory orchid the found in Forest Resort of Way Kanan. This research was conducted in December 2014 until January 2015, using purposive sampling method and using techniques Line transeck, cut the line following the path of the Post traking Right Way to the SRS (Sumatran Rhino Sanctuary, in this research using 4 stations between stations within 2km. The results of this study were obtained 23 species of orchids with 9 genera and 14 species include: the genus Dendrobium, Dendrobium creatceum, Dendrobium crumenatum, genus Grammatophyllum, Grammatophyllum scriptum BL, Agrostophyllum genus, the genus Cymbidium, Cymbidium chloranthum, Cymbidium hartinahianum, Calanthe genus, the genus Bulbophyllum, Arachnis sp, Angraecum didieri, Ascocentrum miniatum, Ascocentrum aureum, Coelogyne foerstermanni, golden shower Oncidium, Phalaenopsis amabilis, Spathoglottis sp. Having analyzed the data obtained it has a value equal to 46% of species density and relative density of 88.36% with a diversity index value of 1.3, based on the criteria of the Shannon-Wienner in Fachrul abundant species diversity can be expressed with the criteria being. The results of this study compiled into resources in conserving germplasm in the form of books and banner summary information, because there are some orchids are found in Forest Resort Way Kanan Balai Way Kambas National Park in Government Regulations  Number. 7 Th.1999 is a protected species of orchids. Orchid is a protected plant species, therefore it is necessary for us to maintain and preserve it.

  10. Spatial extent of analysis influences observed patterns of population genetic structure in a widespread darter species (Percidae)

    Science.gov (United States)

    Argentina, Jane E.; Angermeier, Paul L.; Hallerman, Eric M.; Welsh, Stuart A.

    2018-01-01

    Connectivity among stream fish populations allows for exchange of genetic material and helps maintain genetic diversity, adaptive potential and population stability over time. Changes in species demographics and population connectivity have the potential to permanently alter the genetic patterns of stream fish, although these changes through space and time are variable and understudied in small‐bodied freshwater fish.As a spatially widespread, common species of benthic freshwater fish, the variegate darter (Etheostoma variatum) is a model species for documenting how patterns of genetic structure and diversity respond to increasing isolation due to large dams and how scale of study may shape our understanding of these patterns. We sampled variegate darters from 34 sites across their range in the North American Ohio River basin and examined how patterns of genetic structure and diversity within and between populations responded to historical population changes and dams within and between populations.Spatial scale and configuration of genetic structure varied across the eight identified populations, from tributaries within a watershed, to a single watershed, to multiple watersheds that encompass Ohio River mainstem habitats. This multiwatershed pattern of population structuring suggests genetic dispersal across large distances was and may continue to be common, although some populations remain isolated despite no apparent structural dispersal barriers. Populations with low effective population sizes and evidence of past population bottlenecks showed low allelic richness, but diversity patterns were not related to watershed size, a surrogate for habitat availability. Pairwise genetic differentiation (FST) increased with fluvial distance and was related to both historic and contemporary processes. Genetic diversity changes were influenced by underlying population size and stability, and while instream barriers were not strong determinants of genetic structuring or

  11. Giant Cell Tumour of Tendon Sheath Masquerading As Trigger Finger

    Directory of Open Access Journals (Sweden)

    N Rahimawati

    2010-11-01

    Full Text Available We report a case of a 59-year-old female who presented in the general orthopaedic clinic with triggering of her right middle finger. She did not respond to conventional treatment methods; subsequently she underwent surgical open release under local anaesthesia. Five months postoperatively, the patient presented with signs and symptoms of acute flexor tenosynovitis, and was thought to have a postoperative infection. Re-examination by a hand surgeon raised the possibility of a different aetiology. Based on clinical findings and response to initial treatment, giant cell tumour of the flexor tendon sheath was suspected and later confirmed following surgical biopsy. A high index of suspicion and knowledge of the variegated presentations of giant cell tumour in the hand are beneficial in these types of cases.

  12. Geometry and Mechanics in the Opening of Chiral Seed Pods

    Science.gov (United States)

    Armon, Shahaf; Efrati, Efi; Kupferman, Raz; Sharon, Eran

    2011-09-01

    We studied the mechanical process of seed pods opening in Bauhinia variegate and found a chirality-creating mechanism, which turns an initially flat pod valve into a helix. We studied configurations of strips cut from pod valve tissue and from composite elastic materials that mimic its structure. The experiments reveal various helical configurations with sharp morphological transitions between them. Using the mathematical framework of “incompatible elasticity,” we modeled the pod as a thin strip with a flat intrinsic metric and a saddle-like intrinsic curvature. Our theoretical analysis quantitatively predicts all observed configurations, thus linking the pod’s microscopic structure and macroscopic conformation. We suggest that this type of incompatible strip is likely to play a role in the self-assembly of chiral macromolecules and could be used for the engineering of synthetic self-shaping devices.

  13. Una storia di ‘integrazione’ nella Napoli angioina

    Directory of Open Access Journals (Sweden)

    Rosa Smurra

    2011-06-01

    Full Text Available In every epoch cities have been the landing-place for immigrants. The medieval cities were also places in which have been verified numerous and variegated migratory movements that involved all classes: from economic and political elites to more humble components of social stratification. That which is presented in these pages is certainly an exemplary event, symptomatic of a general picture, all things considered, that is contradictory, giving on the one hand evidence of the persistence of phenomena such as the practice of the commerce of slaves, and on the other hand the social ransoming of an “Ethiopian” slave, by means of its inclusion in the ganglia of the Angevin kingdom and the awarding of high profile bureaucratic offices. In the urban framework of Naples in the first half of the Fourteenth century a human story is unraveled that the tangle of literary and documentary sources with architectural persistencies has made possible to reconstruct.

  14. Structure and Function of SET and MYND Domain-Containing Proteins

    Directory of Open Access Journals (Sweden)

    Nicholas Spellmon

    2015-01-01

    Full Text Available SET (Suppressor of variegation, Enhancer of Zeste, Trithorax and MYND (Myeloid-Nervy-DEAF1 domain-containing proteins (SMYD have been found to methylate a variety of histone and non-histone targets which contribute to their various roles in cell regulation including chromatin remodeling, transcription, signal transduction, and cell cycle control. During early development, SMYD proteins are believed to act as an epigenetic regulator for myogenesis and cardiomyocyte differentiation as they are abundantly expressed in cardiac and skeletal muscle. SMYD proteins are also of therapeutic interest due to the growing list of carcinomas and cardiovascular diseases linked to SMYD overexpression or dysfunction making them a putative target for drug intervention. This review will examine the biological relevance and gather all of the current structural data of SMYD proteins.

  15. Generalized nucleation and looping model for epigenetic memory of histone modifications

    Science.gov (United States)

    Erdel, Fabian; Greene, Eric C.

    2016-01-01

    Histone modifications can redistribute along the genome in a sequence-independent manner, giving rise to chromatin position effects and epigenetic memory. The underlying mechanisms shape the endogenous chromatin landscape and determine its response to ectopically targeted histone modifiers. Here, we simulate linear and looping-driven spreading of histone modifications and compare both models to recent experiments on histone methylation in fission yeast. We find that a generalized nucleation-and-looping mechanism describes key observations on engineered and endogenous methylation domains including intrinsic spatial confinement, independent regulation of domain size and memory, variegation in the absence of antagonists, and coexistence of short- and long-term memory at loci with weak and strong constitutive nucleation. These findings support a straightforward relationship between the biochemical properties of chromatin modifiers and the spatiotemporal modification pattern. The proposed mechanism gives rise to a phase diagram for cellular memory that may be generally applicable to explain epigenetic phenomena across different species. PMID:27382173

  16. Mapping china and managing the world culture, cartography and cosmology in late imperial times

    CERN Document Server

    Smith, Richard J

    2012-01-01

    From the founding of the Qin dynasty in 221 BCE to the present, the Chinese have been preoccupied with the notion of ordering their world. Efforts to create and maintain order are expressed not only in China's bureaucratic institutions and methods of social and economic organization but also in Chinese philosophy, religious and secular ritual, and comprehensive systems of classifying all natural and supernatural phenomena. Mapping China and Managing the World focuses on Chinese constructions of order (zhi) and examines the most important ways in which elites in late imperial China sought to order their vast and variegated world. This book begins by exploring the role of ancient texts and maps as the two prominent symbolic devices that the Chinese used to construct cultural meaning, and looks at how changing conceptions of 'the world' shaped Chinese cartography, whilst both shifting and enduring cartographic practices affected how the Chinese regarded the wider world. Richard J. Smith goes on to examine the si...

  17. Between the Invisible hand and the Invisible Heart. Italian Welfare Restructuring and the Quest for a New Neoliberal Hegemony

    Directory of Open Access Journals (Sweden)

    Davide Caselli

    2016-09-01

    Full Text Available The article looks at the current restructuring of Italian Welfare putting it into perspective with both the structural contradictions of contemporary capitalism and the more contingent and specific condi-tions of contemporary Italy. Relying on the approach taken by the political economy of the Welfare State, the variegated neoliberalization studies and the concept of référentiel, the process is considered and ana-lysed both in its semiotic and material elements. Looking at the transformations of the key-actors in the pre-crisis Welfare Mix arrangement, an on-going entrepreneurial and financial change is identified, open-ing the door to two, complementary, scenarios: a smart-utopia private Welfare one for those able to pay and a repressive residual Welfare state for the poor.

  18. Identifying niche-mediated regulatory factors of stem cell phenotypic state: a systems biology approach.

    Science.gov (United States)

    Ravichandran, Srikanth; Del Sol, Antonio

    2017-02-01

    Understanding how the cellular niche controls the stem cell phenotype is often hampered due to the complexity of variegated niche composition, its dynamics, and nonlinear stem cell-niche interactions. Here, we propose a systems biology view that considers stem cell-niche interactions as a many-body problem amenable to simplification by the concept of mean field approximation. This enables approximation of the niche effect on stem cells as a constant field that induces sustained activation/inhibition of specific stem cell signaling pathways in all stem cells within heterogeneous populations exhibiting the same phenotype (niche determinants). This view offers a new basis for the development of single cell-based computational approaches for identifying niche determinants, which has potential applications in regenerative medicine and tissue engineering. © 2017 The Authors. FEBS Letters published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.

  19. The epigenetic modifier Fam208a is required to maintain epiblast cell fitness

    Czech Academy of Sciences Publication Activity Database

    Bhargava, Shohag; Cox, B.; Polydorou, Ch.; Grešáková, Veronika; Kořínek, Vladimír; Strnad, Hynek; Sedláček, Radislav; Epp, Trevor Allan; Chawengsaksophak, Kallayanee

    2017-01-01

    Roč. 7, léto (2017), č. článku 9322. ISSN 2045-2322 R&D Projects: GA ČR GA15-23165S; GA MŠk LO1419; GA MŠk(CZ) ED1.1.00/02.0109; GA MŠk ED2.1.00/19.0395; GA MŠk(CZ) LM2011032; GA MŠk(CZ) LM2015040; GA MŠk(CZ) LQ1604 Institutional support: RVO:68378050 Keywords : bone morphogenetic protein -4 * position-effect variegation * gastrulating mouse embryo * anterior-posterior axis * mesoderm differentiation * expression pattern * mice lacking * postimplantation development * definitive endoderm * primitive streak Subject RIV: EB - Genetics ; Molecular Biology OBOR OECD: Biochemistry and molecular biology Impact factor: 4.259, year: 2016

  20. Race in California's prison fire camps for men: prison politics, space, and the racialization of everyday life.

    Science.gov (United States)

    Goodman, Philip

    2014-09-01

    The vast majority of social scientists agree that race is "socially constructed." Yet many scholars of punishment and prisons still treat race as static, self-evident categories. One result is that not enough is known about the production, meanings, and consequences of race as experienced by prisoners and those who guard and manage them. The author's research on California's prison fire camps uncovers the micro-level ways in which race is performed and imbued with meaning; he reveals how racial understandings color people and settings. One puzzle is that prisoners in California's fire camps will fight natural disasters side by side, sharing water and provisions, but separate into racial groups when in the camp itself. In part to answer this (and in part to develop better understandings of race and prisons more generally), the author unpacks the variegated nature of punishment and the spatialization of race and advocates for research that is faithful to the constructivist framework.

  1. [Advances in understanding Drosophila salivary gland polytene chromosome and its applications in genetics teaching].

    Science.gov (United States)

    Li, Gang; Chen, Fan-guo

    2015-06-01

    Drosophila salivary gland polytene chromosome, one of the three classical chromosomes with remarkable characteristics, has been used as an outstanding model for a variety of genetic studies since 1934. The greatest contribution of this model to genetics has been providing extraordinary angle of view in studying interphase chromosome structure and gene expression regulation. Additionally, it has been extensively used to understand some special genetic phenomena, such as dosage compensation and position-effect variegation. In this paper, we briefly review the advances in the study of Drosophila salivary gland chromosome, and try to systematically and effectively introduce this model system into genetics teaching practice in order to steer and inspire students' interest in genetics.

  2. Small cell extraskeletal osteosarcoma: a rare case report

    Directory of Open Access Journals (Sweden)

    Neelam Sood

    2014-01-01

    Full Text Available Extraskeletal osteosarcoma is a rare malignant mesenchymal neoplasm and its small cell variant is one among the rarest variant. This article describes a 60-year-old woman presenting with a large, lobulated, painful mass in left thigh with associated history of trauma since 18 months. Her magnetic resonance imaging showed a variegated mixed intensity lesion with associated cystic degeneration, necrosis and matrix arborizing nearby muscles. Fine needle aspiration cytology showed a small cell lesion with very scant osteoid. Tumor was excised and histopathological diagnosis was small cell osteosarcoma involving adjacent muscles and fat with sparing of lymph nodes. The aim of this article is to present the clinical, radiological, cyto-histological and immunohistochemical features of this extremely rare lesion.

  3. Transcriptome analysis of the phytobacterium Xylella fastidiosa growing under xylem-based chemical conditions.

    Science.gov (United States)

    Ciraulo, Maristela Boaceff; Santos, Daiene Souza; Rodrigues, Ana Claudia de Freitas Oliveira; de Oliveira, Marcus Vinícius; Rodrigues, Tiago; de Oliveira, Regina Costa; Nunes, Luiz R

    2010-01-01

    Xylella fastidiosa is a xylem-limited bacterium responsible for important plant diseases, like citrus-variegated chlorosis (CVC) and grapevine Pierce's disease (PD). Interestingly, in vitro growth of X. fastidiosa in chemically defined media that resemble xylem fluid has been achieved, allowing studies of metabolic processes used by xylem-dwelling bacteria to thrive in such nutrient-poor conditions. Thus, we performed microarray hybridizations to compare transcriptomes of X. fastidiosa cells grown in 3G10-R, a medium that resembles grape sap, and in Periwinkle Wilt (PW), the complex medium traditionally used to cultivate X. fastidiosa. We identified 299 transcripts modulated in response to growth in these media. Some 3G10R-overexpressed genes have been shown to be upregulated in cells directly isolated from infected plants and may be involved in plant colonization, virulence and environmental competition. In contrast, cells cultivated in PW show a metabolic switch associated with increased aerobic respiration and enhanced bacterial growth rates.

  4. MRI prognostic value in multiple myeloma

    International Nuclear Information System (INIS)

    Medina, G.; Mazzuco, J.; Corrado, C.; Hendler, M.

    2001-01-01

    Purpose: To evaluate the prognosis value of the bone marrow (BM) MR infiltration patterns in patients with MM, without treatment, and to assess the results altogether with other prognosis factors (clinical, laboratory, radiology). Materials and methods: MR was performed in 21 patients with MM. Bone lesions, plasmacytomas and osteoporosis were assessed with skeletal radiographs. Results: MR of patients with MM revealed 3 abnormal BM involvement patterns: focal (FP), diffuse (DP) and variegated (VP). Unlike others reported series, we identified MR studies with 2 or more MR patterns in 13 patients. We define this particular type as mixed pattern (MP). Conclusion: MR is more sensitive than conventional radiology in detecting bone lesions. The MR patterns combined with the other conventional staging factors provide a useful tool for disease prognosis. In our series, patients with DP, alone or combined with other patterns (mixed), were related with the worst prognosis. (author)

  5. Better science and better race? Social Darwinism and Chinese eugenics.

    Science.gov (United States)

    Chung, Yuehtsen Juliette

    2014-12-01

    This essay explores the variegated roles played by racial, eugenic, and Social Darwinist discourse in China over roughly the last century. Using Japan as a parallel for comparison, it analyzes the introduction of the term "eugenics" into Japanese and Chinese. It then locates the deployment of eugenics and Social Darwinism as counterimperial discourse in East Asia. It offers a brief history of eugenics thinking in China across the twentieth century, focusing on the Chinese eugenicist Pan Guangdan, who used race as a category of analysis yet without any sense of hierarchy. He was critically aware of the scientific basis of eugenics and helped craft the study of eugenics in China, from biology to sociology, from economics to ethnology.

  6. Distribution of the Sunda Colugo (Galeopterus variegatus) in Malaysia (Peninsular, Sabah, Sarawak).

    Science.gov (United States)

    Nasir, Muhammad Dzulhelmi Muhammad; Abdullah, Mohd Tajuddin

    2010-12-01

    There is not much information available on the distribution of the Sunda colugo (Galeopterus variegates) in Malaysia, despite it being one of only two known species in the order Dermoptera. Data on the presence of the Sunda colugo and the vernacular names used by various ethnic groups throughout Malaysia were collected and compiled from various primary and secondary sources. There were 27 locations from Peninsular, 11 locations from Sabah and 34 locations from Sarawak that reported the presence of the Sunda colugo throughout Malaysia. The various ethnic groups of Malaysia adopted 37 different vernacular names to describe the Sunda colugo. This baseline data can be useful for the management authorities in conducting periodic monitoring and will enhance our knowledge of the population dynamics of the Sunda colugo in Malaysia.

  7. Reading the Conjuncture

    DEFF Research Database (Denmark)

    Flohr, Mikkel; Harrison, Yannick Nehemiah Antonio

    2016-01-01

    This interview with Bob Jessop covers diverse issues ranging from the challenge of defining the state to problems of conjunctural analysis and political practice. It first explores the state and state power from a strategic-relational perspective and then addresses the relation between economics...... and politics in the context of a tendentially unified world market and a continuing plurality of national states. Attention then turns to the North Atlantic financial crisis and the Eurozone crisis and their relation to a variegated global capitalism organized in the shadow of neoliberalism. Financialization...... and the rise of political capitalism are seen as leading to a loss of temporal sovereignty, problems of crisis management, an assault on democracy, and the rise of the austerity state. Closing remarks address the role of left-wing social movements and parties, such as SYRIZA and Podemos, and the difficulties...

  8. Writings from the Margins: German-Jewish Women Poets from the Bukovina

    Directory of Open Access Journals (Sweden)

    Amy Colin

    1997-01-01

    Full Text Available Emerging at the crossroads of heterogeneous languages and cultures, German-Jewish women's poetry from the Bukovina displays the characteristics of its fascinating multilingual contextuality, yet it also bears the stigma of a double marginalization, for its representatives became time and again targets of both anti-Semitic attacks as well as gender discrimination. The present essay explores the untiring struggles of German-Jewish women authors from the Bokovina for acceptance within the Jewish and non-Jewish community. It analyzes their attempts to cope with social barriers, prejudices, and their difficult situation as both women and Jews. The essay also sets their poetry against the background of their multilingual contextuality. It is the Bukovinian biotope, where Ruthenians, Romanians, Germans, Jews, Armenians, Magyars, Poles, Lipovanes, and Hutsuls peacefully coexisted for many centuries, producing a variegated Romanian, Ruthenian, Austro-German, German-Jewish, and Yiddish literature as well as poets who were fluent in several languages.

  9. BRICS: an explanation in critical geography

    Directory of Open Access Journals (Sweden)

    Krishnendra Meena

    2013-12-01

    Full Text Available BRICS, an abbreviation for Brazil, Russia, India, China and South Africa, a group of five influential and emerging economies successfully completed its fifth annual summit in Durban during March 26-27, 2013. A significantly unique feature of the group is its geographical spread as evident from the location of these five constituent states which are situated in four continents. The paper seeks to explain the phenomenon of the disparate group BRICS through literature in Critical Geography as it is understood that the recent phase of globalization has created spatial patterns which were hitherto not experienced and therefore not clearly recognized in the literature on International Relations and traditional geography. Such spatially variegated groupings like the BRICS could be analyzed and interpreted in Critical Geography and Critical Geopolitics literature through three important concepts: a Space b Geographical and Geopolitical Imaginations and c Region. The paper seeks to explain BRICS through these conceptual tools.

  10. The morphology of the Martian surface

    International Nuclear Information System (INIS)

    Carr, M.H.

    1980-01-01

    Most of the southern hemisphere of Mars is densely cratered and stands 1-3 km above the topographic datum. The northern hemisphere is more sparsely cratered and elevations are generally below the datum. A broad rise, the Tharsis bulge, centered at 14 0 S, 101 0 W, is 8000 km across and 10 km above the datum at its summit. The densely cratered terrain has two main components; very ancient crust, nearly saturated with large craters, and younger intercrater plains. In many areas the older unit is fractured and extensively dissected by small channels. The younger intercrater plains are distinctly layered in places and less dissected, less fractured, and less cratered. Both units probably date from very early in the planet's history. Cratered plains cover much of the northern hemisphere and are highly variegated. (orig.)

  11. Lupus community panel proposals for optimising clinical trials: 2018

    Science.gov (United States)

    Merrill, Joan T; Manzi, Susan; Aranow, Cynthia; Askenase, Anca; Bruce, Ian; Chakravarty, Eliza; Chong, Ben; Costenbader, Karen; Dall’Era, Maria; Ginzler, Ellen; Hanrahan, Leslie; Kalunian, Ken; Merola, Joseph; Raymond, Sandra; Rovin, Brad; Saxena, Amit; Werth, Victoria P

    2018-01-01

    Formidable impediments stand in the way of treatment development for lupus. These include the unwieldy size of current trials, international competition for scarce patients, complex outcome measures and a poor understanding of these outcomes in the world at large. The heterogeneity of the disease itself coupled to superimposition of variegated background polypharmacy has created enough immunological noise to virtually ensure the failure of lupus treatment trials, leaving an understandable suspicion that at least some of the results in testing failed drugs over the years may not have been negative, but merely uninterpretable. The authors have consulted with many clinical trial investigators, biopharmaceutical developers and stakeholders from government and voluntary sectors. This paper examines the available evidence that supports workable trial designs and proposes approaches to improve the odds of completing interpretable treatment development programs for lupus. PMID:29657738

  12. Interpretation of lightcurves of atmosphereless bodies. I - General theory and new inversion schemes

    Science.gov (United States)

    Kaasalainen, M.; Lamberg, L.; Lumme, K.; Bowell, E.

    1992-01-01

    Theoretical models of light-curve inversion that can be used in photomorphography, i.e., determination of the 3D shape and/or the light-scattering behavior of the surface of a body from disk-integrated photometry. These methods can be applied to atmosphereless bodies, mainly asteroids and planetary satellites, in the solar system. The inversion comprises three steps. First, a function containing data on the shape and the albedo variegation of an asteroid is determined. In the second step, separate expressions for the inverse of the Gaussian curvature and the albedo distribution are derived from the information obtained in the first step. In the third step, the nontrivial problem of determining the radius vector of the surface from the Gaussian curvature is solved by using iterative optimization procedures.

  13. Incorporating Colour Information for Computer-Aided Diagnosis of Melanoma from Dermoscopy Images: A Retrospective Survey and Critical Analysis

    Directory of Open Access Journals (Sweden)

    Ali Madooei

    2016-01-01

    Full Text Available Cutaneous melanoma is the most life-threatening form of skin cancer. Although advanced melanoma is often considered as incurable, if detected and excised early, the prognosis is promising. Today, clinicians use computer vision in an increasing number of applications to aid early detection of melanoma through dermatological image analysis (dermoscopy images, in particular. Colour assessment is essential for the clinical diagnosis of skin cancers. Due to this diagnostic importance, many studies have either focused on or employed colour features as a constituent part of their skin lesion analysis systems. These studies range from using low-level colour features, such as simple statistical measures of colours occurring in the lesion, to availing themselves of high-level semantic features such as the presence of blue-white veil, globules, or colour variegation in the lesion. This paper provides a retrospective survey and critical analysis of contributions in this research direction.

  14. Die uitbouing van die Bybelse kanon in antieke Judaïsme en die vroeë Christendom

    Directory of Open Access Journals (Sweden)

    Jörg Frey

    2015-07-01

    Full Text Available The development of the biblical canon in ancient Judaism and early Christianity. A brief account of the process of the development of both the Jewish and the bipartite Christian canon is given. It is argued that due to insights gained from recent textual discoveries, especially the Dead Sea Scrolls (Qumran texts, earlier theories about the history of canonisation had to be reviewed. With the New Testament canon the authors focus on the influence of Marcion as well as the various other factors that played a role in the process of canonisation. It is shown that canonisation was the result of a complicated and variegated canonical process. But in spite of the problems of the criteria used and other factors involved, the biblical canon is theologically valuable and ‘well-chosen’.

  15. Filiform serrated adenomatous polyposis arising in a diverted rectum of an inflammatory bowel disease patient

    DEFF Research Database (Denmark)

    Klarskov, Louise; Mogensen, Anne Mellon; Jespersen, Niels

    2011-01-01

    Klarskov L, Mogensen AM, Jespersen N, Ingeholm P, Holck S. Filiform serrated adenomatous polyposis arising in a diverted rectum of an inflammatory bowel disease patient. APMIS 2011; 119: 393-8. A 54-year-old man, previously colectomized for inflammatory bowel disease, developed carcinoma in the i......Klarskov L, Mogensen AM, Jespersen N, Ingeholm P, Holck S. Filiform serrated adenomatous polyposis arising in a diverted rectum of an inflammatory bowel disease patient. APMIS 2011; 119: 393-8. A 54-year-old man, previously colectomized for inflammatory bowel disease, developed carcinoma...... during the adenoma carcinoma sequence included the acquisition of CK7 expression in the malignant portion. Gastric mucin may play a role in the initial step of the neoplastic evolution and CK7 may denote neoplastic progression. This case confirms the notion of a widely variegated morphology of precursor...

  16. Mutation induction, evaluation and utilization for development of high yielding varieties in Indian mustard and sunflower: an overview of BARC work

    International Nuclear Information System (INIS)

    Jambhulkar, S.J.; Shitre, A.S.

    2009-01-01

    Mutation breeding programme in Indian mustard and sunflower at BARC has resulted into the development of wide spectrum of mutations for seed coat colour, chlorophyll, plant height, maturity, flower morphology, seed weight and oil content. In Indian mustard, TM1 and TM50 are high yielding yellow seed coat mutants, which were exploited in hybridisation to develop bold, yellow seed coat and high yielding genotypes. Light green leaf and variegated leaf are novel mutation in mustard. Putative mutants for drought tolerance have been isolated. Variability for zero erucic acid and zero glucosinolates genotypes have been developed in B. napus and B. juncea. In sunflower, high yielding black seed coat mutant were isolated. Extreme dwarf measuring only 11 cm is novel. Three high yielding varieties namely TM2, TM4, and TPM1 in mustard and one i.e.TAS82 in sunflower have been released for cultivation in collaboration with state agricultural universities. (author)

  17. Agrogenic degradation of soils in Krasnoyarsk forest-steppe

    Science.gov (United States)

    Shpedt, A. A.; Trubnikov, Yu. N.; Zharinova, N. Yu.

    2017-10-01

    Agrogenic degradation of soils in Krasnoyarsk forest-steppe was investigated. Paleocryogenic microtopography of microlows and microhighs in this area predetermined the formation of paragenetic soil series and variegated soil cover. Specific paleogeographic conditions, thin humus horizons and soil profiles, and long-term agricultural use of the land resulted in the formation of soils unstable to degradation processes and subjected to active wind and water erosion. Intensive mechanical soil disturbances during tillage and long-term incorporation of the underlying Late Pleistocene (Sartan) calcareous silty and clay loams into the upper soil horizons during tillage adversely affected the soil properties. We determined the contents of total and labile humus and easily decomposable organic matter and evaluated the degree of soil exhaustion. It was concluded that in the case of ignorance of the norms of land use and soil conservation practices, intense soil degradation would continue leading to complete destruction of the soil cover within large areas.

  18. Crystallization and preliminary X-ray analysis of stationary phase survival protein E (SurE) from Xylella fastidiosa in two crystal forms

    International Nuclear Information System (INIS)

    Reis, Marcelo Augusto dos; Saraiva, Antonio Marcos; Santos, Marcelo Leite dos; Souza, Anete Pereira de; Aparicio, Ricardo

    2012-01-01

    The crystallization and preliminary analysis of two crystal forms of survival protein E from X. fastidiosa are reported. The bacterium Xylella fastidiosa is a phytopathogenic organism that causes citrus variegated chlorosis, a disease which attacks economically important crops, mainly oranges. In this communication, the crystallization and preliminary X-ray crystallographic analysis of XfSurE, a survival protein E from X. fastidiosa, are reported. Data were collected for two crystal forms, I and II, to 1.93 and 2.9 Å resolution, respectively. Crystal form I belonged to space group C2, with unit-cell parameters a = 172.36, b = 84.18, c = 87.24 Å, α = γ = 90, β = 96.59°, whereas crystal form II belonged to space group C2, with unit-cell parameters a = 88.05, b = 81.26, c = 72.84 Å, α = γ = 90, β = 94.76°

  19. The Genus Heliopsis: Development of Varieties and Their Use in the European Gardens After the Mid 19th Century

    Directory of Open Access Journals (Sweden)

    Jiří Uher

    2014-01-01

    Full Text Available This review summarizes data on the development of varieties in historic gardens of the once very popular Ox-eyes (Heliopsis Pers., Asteraceae: Zinniinae after the mid 19th century, with regard to the development of varietal assortments in the periods corresponding to the most important architectural styles and to their fluctuating popularity. Old varietal assortments, usually derived from large-flowered H. helianthoides var. scabra, now rapidly disappear and the oldest varieties, including the once famous Lemoine’s selections, are virtually inaccessible. Until recently the most propagated Götz’s and Förster’s varieties also disappear and are replaced by modern, relatively small-flowered selections delivered from H. helianthoides var. helianthoides or patent protected variegated varieties. Neither of these groups, however, is applicable to the restoration of historic gardens. Tables show data on the origin of about eighty both still cultivated and vanished varieties.

  20. Art technologies as possible propulsars in technological innovation

    DEFF Research Database (Denmark)

    Jørgensen, Lars Botin

    2003-01-01

    kind. This discussion, of general character, constitutes the initial part of this paper. Then it briefly looks into the final terms of the papertitle and try to relate to the diffuse and discussed technological innovation. Both aspects – art and technology and technological innovation – will be dealt...... with from a historical/hermeneutic and social constructivist point of view, as the paper moves from a general principal level to a more specific, exemplary level, where three different art technologies are presented as possible propulsars in technological innovation.......Art and technology has, as the German philosopher Martin Heidegger points out, the same etymological root. A thorough investigation of the relationship between art and technology will show how this same root has manifested itself in different times and spaces bringing results of the most variegated...

  1. Sexual dimorphism in white campion: complex control of carpel number is revealed by Y chromosome deletions

    International Nuclear Information System (INIS)

    Lardon, A.; Georgiev, S.; Aghmir, A.; Le Merrer, G.; Negrutiu, I.

    1999-01-01

    Sexual dimorphism in the dioecious plant white campion (Silene latifolia = Melandrium album) is under the control of two main regions on the Y chromosome. One such region, encoding the gynoecium-suppressing function (GSF), is responsible for the arrest of carpel initiation in male flowers. To generate chromosomal deletions, we used pollen irradiation in male plants to produce hermaphroditic mutants (bsx mutants) in which carpel development was restored. The mutants resulted from alterations in at least two GSF chromosomal regions, one autosomal and one located on the distal half of the (p)-arm of the Y chromosome. The two mutations affected carpel development independently, each mutation showing incomplete penetrance and variegation, albeit at significantly different levels. During successive meiotic generations, a progressive increase in penetrance and a reduction in variegation levels were observed and quantified at the level of the Y-linked GSF (GSF-Y). Possible mechanisms are proposed to explain the behavior of the bsx mutations: epigenetic regulation or/and second-site mutation of modifier genes. In addition, studies on the inheritance of the hermaphroditic trait showed that, unlike wild-type Y chromosomes, deleted Y chromosomes can be transmitted through both the male and the female lines. Altogether, these findings bring experimental support, on the one hand, to the existence on the Y chromosome of genic meiotic drive function(s) and, on the other hand, to models that consider that dioecy evolved through multiple mutation events. As such, the GSF is actually a system containing more than one locus and whose primary component is located on the Y chromosome

  2. Short-Term and Long-Term Biological Effects of Chronic Chemical Contamination on Natural Populations of a Marine Bivalve.

    Directory of Open Access Journals (Sweden)

    Marine Breitwieser

    Full Text Available Understanding the effects of chronic chemical contamination on natural populations of marine organisms is complex due to the combined effects of different types of pollutants and environmental parameters that can modulate the physiological responses to stress. Here, we present the effects of a chronic contamination in a marine bivalve by combining multiple approaches that provide information on individual and population health. We sampled variegated scallops (Mimachlamys varia at sites characterized by different contaminants and contamination levels to study the short and long-term (intergenerational responses of this species to physiological stress. We used biomarkers (SOD, MDA, GST, laccase, citrate synthase and phosphatases as indicators of oxidative stress, immune system alteration, mitochondrial respiration and general metabolism, and measured population genetic diversity at each site. In parallel, concentration of 14 trace metals and 45 organic contaminants (PAHs, PCBs, pesticides in tissues were measured. Scallops were collected outside and during their reproductive season to investigate temporal variability in contaminant and biomarker levels. Our analyses revealed that the levels of two biomarkers (Laccase-type phenoloxidase and malondialdehyde were significantly correlated with Cd concentration. Additionally, we observed significant seasonal differences for four of the five biomarkers, which is likely due to the scallop reproductive status at time of sampling. As a source of concern, a location that was identified as a reference site on the basis of inorganic contaminant levels presented the same level of some persistent organic pollutants (DDT and its metabolites than more impacted sites. Finally, potential long-term effects of heavy metal contamination were observed for variegated scallops as genetic diversity was depressed in the most polluted sites.

  3. Stratigraphy and structure of the Miners Mountain area, Wayne County, Utah

    Science.gov (United States)

    Luedke, Robert G.

    1953-01-01

    The Miners Mountain area includes about 85 square miles in Wayne County, south-central Utah. The area is semiarid and characterized by cliffs and deep canyons. Formations range in age from Permian to Upper Jurassic and have an aggregate thickness of about 3,500 feet. Permian formations are the buff Coconino sandstone and the overlying white, limy, shert-containing Kaibab limestone. Unconformably overlying the Kaihab is the lower Triassic Moenkopi formation of reddish-brown and yellow mudstone, siltstone, and sandstone; it contains the Sinbad limestone member (?) in the lower part. Thin, lenticular Shinarump conglomerate unconformably overlies the Moenkopi, but grades upward into the Upper Triassic Chinle formation of variegated mudstone with some interbedded sandstone and limestone lenses. Uncomformably overlying the Chinle are the Wingate sandstone, Kayenta formation, and Navajo sandstone of the Jurassic (?) Glen Canyon group, which consist of red to white sandstone. Only the lower part of the Carmel formation of the Upper Jurassic San Rafael group is exposed in the area; it consists of variegated siltstone, sandstone, limestone, and gypsum. The conspicuous structural feature in the area is the Teasdale anticline which trends northwest, is about 14 miles long, and is asymmetric with a steeper west flank. Bounding the anticline on the northeast and east is the Capitol Reef monocline, the northern part of the Waterpocket Fold. Strata in the area are broken by steeply-dipping normal faults with small displacements, except for the Teasdale fault which has a maximum displacement of over 1,000 feet. Jointing is prominent in some formations. The major orogenic movement in the area is believed to be late Upper Cretaceous to early Tertiary. Epeirogenic uplift occurred intermittently throughout Tertiary and perhaps Quaternary time.

  4. Morphological differentiation of Streptomyces viridochromogenes E-219 on solid culture

    International Nuclear Information System (INIS)

    Liang Xinle; Zhu Jing; Jin Yingyan

    2012-01-01

    The Streptomyces viridochromogenes E-219 was derived from Streptomyces viridochromogenes CGMCC4.1119 treated with 60 Co γ-rays irradiation and protoplast fusion. With the help of fluorescent probes, fluorescence microscope and electron microscopy, the morphology and development of E-219 on solid surface culture were investigated in this study. The effect of agarslant culture time on the production of Avilamycin was also studied to provide theoretical basis for industrial fermentation of selecting the appropriate seed to culture on the agarslant culture medium. The results implied that the development of colonies of Streptomyces viridochromogenes accompanied the intermittent hyhae apoptosis, and the production of spores was from the active mycelium. The colonial morphology of strain E-219 was significantly different from the original strain CGMCC4h1119. There were variegated hyphae formation in the stage of spore germination and initial hyphae development (10 h) with the live and dead segments alternated in a highly regular fashion within the same hypha. After the early single colony formation, the third phase was followed by profuse growth of the live segments derived from the variegated hypha, then the second apoptosis of the mycelia (48 h) was occurred with another quick growth, and sporulation was occurred at 96 h. Strain CGMCC4.1119 had spiral sporotrichial and round conidiophores with spike, whereas strain E-219 had linear sporotrichial, smooth and dylindrical conidiophore. The results of shake flask experiments indicated that the spores of E-219 had that highest activity when cultured on agarslant culture medium and incubated for 106 h with the production of avilamycin up to 1200 mg/L. (authors)

  5. Short-Term and Long-Term Biological Effects of Chronic Chemical Contamination on Natural Populations of a Marine Bivalve.

    Science.gov (United States)

    Breitwieser, Marine; Viricel, Amélia; Graber, Marianne; Murillo, Laurence; Becquet, Vanessa; Churlaud, Carine; Fruitier-Arnaudin, Ingrid; Huet, Valérie; Lacroix, Camille; Pante, Eric; Le Floch, Stéphane; Thomas-Guyon, Hélène

    2016-01-01

    Understanding the effects of chronic chemical contamination on natural populations of marine organisms is complex due to the combined effects of different types of pollutants and environmental parameters that can modulate the physiological responses to stress. Here, we present the effects of a chronic contamination in a marine bivalve by combining multiple approaches that provide information on individual and population health. We sampled variegated scallops (Mimachlamys varia) at sites characterized by different contaminants and contamination levels to study the short and long-term (intergenerational) responses of this species to physiological stress. We used biomarkers (SOD, MDA, GST, laccase, citrate synthase and phosphatases) as indicators of oxidative stress, immune system alteration, mitochondrial respiration and general metabolism, and measured population genetic diversity at each site. In parallel, concentration of 14 trace metals and 45 organic contaminants (PAHs, PCBs, pesticides) in tissues were measured. Scallops were collected outside and during their reproductive season to investigate temporal variability in contaminant and biomarker levels. Our analyses revealed that the levels of two biomarkers (Laccase-type phenoloxidase and malondialdehyde) were significantly correlated with Cd concentration. Additionally, we observed significant seasonal differences for four of the five biomarkers, which is likely due to the scallop reproductive status at time of sampling. As a source of concern, a location that was identified as a reference site on the basis of inorganic contaminant levels presented the same level of some persistent organic pollutants (DDT and its metabolites) than more impacted sites. Finally, potential long-term effects of heavy metal contamination were observed for variegated scallops as genetic diversity was depressed in the most polluted sites.

  6. KARAKTERISTIK TANAH DI BAWAH TEGAKAN JENIS VEGETASI MANGROVE DAN KEDALAMAN TANAH BERBEDA SEBAGAI INDIKATOR BIOLOGIS UNTUK TANAH TAMBAK DI KABUPATEN MAMUJU PROVINSI SULAWESI BARAT

    Directory of Open Access Journals (Sweden)

    Akhmad Mustafa

    2011-04-01

    Full Text Available Kondisi lahan mangrove sangat ekstrem, sehingga vegetasi yang tumbuh merupakan vegetasi yang telah beradaptasi dan berevolusi dengan kondisi tersebut. Oleh karena itu, dilakukan penelitian yang bertujuan untuk mengetahui karakteristik tanah di bawah tegakan vegetasi mangrove dan kedalaman tanah berbeda yang dapat dijadikan indikator biologis untuk memprediksi karakteristik tanah untuk budidaya tambak. Pengukuran dan pengambilan contoh tanah dilakukan di bawah tegakan paku laut (Acrostichum aureum, bakau (Rhizophora apiculata, api-api (Avicennia alba, dan nipah (Nypa fruticans masing-masing pada kedalaman tanah 0-0,25 m dan 0,50-0,75 m di hutan mangrove Kabupaten Mamuju Provinsi Sulawesi Barat. Kualitas tanah yang diukur langsung di lapangan adalah pHF, pHFOX, dan potensial redoks, sedangkan yang dianalisis di laboratorium adalah kandungan air, pHKCl, pHOX, SP, SKCl, SPOS, TPA, TAA, TSA, pirit, karbon organik, N-total, PO4, Fe, Al, tekstur, dan nilai n. Analisis ragam dilakukan untuk mengetahui perbedaan kualitas tanah antar vegetasi mangrove pada kedalaman yang sama, sedangkan Uji T dilakukan untuk mengetahui perbedaan kualitas tanah antar kedalaman pada vegetasi mangrove yang sama. Hasil penelitian menunjukkan bahwa tegakan bakau, api-api, nipah, dan paku laut yang tumbuh pada tanah sulfat masam Kabupaten Mamuju diklasifikasikan sebagai Sulfaquent dan Sulfihemits untuk kategori Kelompok Besar. pHF tanah pada vegetasi dan kedalaman tanah yang berbeda relatif sama, tetapi peubah kemasaman tanah lainnya menunjukkan bahwa tanah vegetasi paku laut memiliki potensi kemasaman yang lebih rendah dibandingkan dengan vegetasi lainnya. Kesuburan dan sifat fisik tanah vegetasi paku laut lebih mendukung untuk lahan budidaya tambak daripada vegetasi lainnya (bakau, api-api, nipah. Kualitas tanah pada setiap vegetasi relatif sama pada kedua kedalaman, kecuali tanah vegetasi paku laut dan api-api yang memiliki pH dan kandungan PO4 yang

  7. Stratigraphy and palaeoenvironment of the Lower-``middle'' Oligocene units in the northern part of the Western Taurides (İncesu area, Isparta, Turkey)

    Science.gov (United States)

    Akkiraz, Mehmet Serkan; Akgün, Funda; Örçen, Sefer

    2011-01-01

    . Terrestrial palynomorphs in more inland settings were transported by running water towards the sea. Conifers are represented by poorly preserved and rare pollen grains of Pityosporites, Cathayapollis and Piceapollis which may have been transported by wind. In this study, the terrestrial climate of the İncesu Formation is also discussed on the basis of the Coexistence Approach method. The climate was warm at the coast (over 20 °C), as evidenced by A.aureum and lepidocaryoid palms, whereas there was a mean annual temperature of 17.2-17.4 °C must be assumed for the upland environment(s).

  8. ANALISIS POTENSI SIMPANAN KARBON HUTAN MANGROVE DI AREA PT. INDOCEMENT TUNGGAL PRAKARSA, TBK P 12 TARJUN

    Directory of Open Access Journals (Sweden)

    Eva Ariani

    2016-12-01

    Full Text Available This study aims to analyze the structure of the mangrove forest stands in a conservation area PT. Indocement P12 Tarjun, South Kalimantan. To determine the potential amount of carbon stored in vegetation and soils di area mangrove forests. Merumuskah form of management in accordance with mangrove forests owned by PT Indocement Tunggal Tbk P 12 Tarjun. The results of this study show the type of vegetation that are within the plot observation is Aegicera corniculatum, Acivennia alba, Avicennia lanata, Avicennia Marina, Bruguera gymnorrhiza, Bruguiera parviflora, Ceriops decandra, Lumnitzera, Rhizophora apiculate, Rhizophora mucronata, Scyphiphora hydrophyllacea, Sonneratia alba, Xylocarpus granatum, Acanthus ebracteatus, Hibiscus tiliaceus, Nypa fruticans, Terminalia catappa and type of vegetation present in the area of research, but is not included in the observation plot is Acrostichum aureum Linn, Pandanus odoratissima. Importance Value Index (IVI lenata kind Avicennia highest growth rate of the tree that is 80,2%, Rhizophora apiculata at the level of the pole that is 57%, and Nypa fruticans at the seedling stage is 20,8%. To Nypa fruticans, though IVIs largest/highest species of plants in the seedling stage but this type is only found on two research plots only. Total carbon content was found at the study site was at 714,77 per hectare. The study was conducted on biomass carbon on the surface, lower plants, nekromassa (dead trees, and mangrove sediments (ground. Carbon sequestration at a site can be seen from the height and diameter of trees while a vegetation type had no significant effect on the absorption of carbon. Environmental management in the form of replanting are research needs to be done to avoid more severe harm than good because of logging by humans atupun affected by coastal erosion. Type Lumnitzera, Scyphiphora hydrophyllacea, Ceriops decandra should get more attention because it has a very low INP hat is necessary to manage

  9. Auream Chaos

    Science.gov (United States)

    2005-01-01

    [figure removed for brevity, see original site] The THEMIS VIS camera is capable of capturing color images of the Martian surface using five different color filters. In this mode of operation, the spatial resolution and coverage of the image must be reduced to accommodate the additional data volume produced from using multiple filters. To make a color image, three of the five filter images (each in grayscale) are selected. Each is contrast enhanced and then converted to a red, green, or blue intensity image. These three images are then combined to produce a full color, single image. Because the THEMIS color filters don't span the full range of colors seen by the human eye, a color THEMIS image does not represent true color. Also, because each single-filter image is contrast enhanced before inclusion in the three-color image, the apparent color variation of the scene is exaggerated. Nevertheless, the color variation that does appear is representative of some change in color, however subtle, in the actual scene. Note that the long edges of THEMIS color images typically contain color artifacts that do not represent surface variation. This false color image was collected during Southern Fall and shows part of the Aureum Chaos. Image information: VIS instrument. Latitude -3.6, Longitude 332.9 East (27.1 West). 35 meter/pixel resolution. Note: this THEMIS visual image has not been radiometrically nor geometrically calibrated for this preliminary release. An empirical correction has been performed to remove instrumental effects. A linear shift has been applied in the cross-track and down-track direction to approximate spacecraft and planetary motion. Fully calibrated and geometrically projected images will be released through the Planetary Data System in accordance with Project policies at a later time. NASA's Jet Propulsion Laboratory manages the 2001 Mars Odyssey mission for NASA's Office of Space Science, Washington, D.C. The Thermal Emission Imaging System (THEMIS

  10. Salt lake Laguna de Fuente de Piedra (S-Spain) as Late Quaternary palaeoenvironmental archive

    Science.gov (United States)

    Höbig, Nicole; Melles, Martin; Reicherter, Klaus

    2014-05-01

    This study deals with Late Quaternary palaeoenvironmental variability in Iberia reconstructed from terrestrial archives. In southern Iberia, endorheic basins of the Betic Cordilleras are relatively common and contain salt or fresh-water lakes due to subsurface dissolution of Triassic evaporites. Such precipitation or ground-water fed lakes (called Lagunas in Spanish) are vulnerable to changes in hydrology, climate or anthropogenic modifications. The largest Spanish salt lake, Laguna de Fuente de Piedra (Antequera region, S-Spain), has been investigated and serves as a palaeoenvironmental archive for the Late Pleistocene to Holocene time interval. Several sediment cores taken during drilling campaigns in 2012 and 2013 have revealed sedimentary sequences (up to 14 m length) along the shoreline. A multi-proxy study, including sedimentology, geochemistry and physical properties (magnetic susceptibility) has been performed on the cores. The sedimentary history is highly variable: several decimetre thick silty variegated clay deposits, laminated evaporites, and even few-centimetre thick massive gypsum crystals (i.e., selenites). XRF analysis was focussed on valuable palaeoclimatic proxies (e.g., S, Zr, Ti, and element ratios) to identify the composition and provenance of the sediments and to delineate palaeoenvironmental conditions. First age control has been realized by AMS-radiocarbon dating. The records start with approximately 2-3 m Holocene deposits and reach back to the middle of MIS 3 (GS-3). The sequences contain changes in sedimentation rates as well as colour changes, which can be summarized as brownish-beige deposits at the top and more greenish-grey deposits below as well as highly variegated lamination and selenites below ca. 6 m depth. The Younger Dryas, Bølling/Allerød, and the so-called Mystery Interval/Last Glacial Maximum have presumably been identified in the sediment cores and aligned to other climate records. In general, the cores of the Laguna de

  11. Pediatric melanoma: incidence, treatment, and prognosis

    Directory of Open Access Journals (Sweden)

    Saiyed FK

    2017-04-01

    Full Text Available Faiez K Saiyed,1 Emma C Hamilton,1 Mary T Austin,1,2 1Department of Pediatric Surgery, McGovern Medical School, 2Department of Surgical Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA Abstract: The purpose of this review is to outline recent advancements in diagnosis, treatment, and prevention of pediatric melanoma. Despite the recent decline in incidence, it continues to be the deadliest form of skin cancer in children and adolescents. Pediatric melanoma presents differently from adult melanoma; thus, the traditional asymmetry, border irregularity, color variegation, diameter >6 mm, and evolution (ABCDE criteria have been modified to include features unique to pediatric melanoma (amelanotic, bleeding/bump, color uniformity, de novo/any diameter, evolution of mole. Surgical and medical management of pediatric melanoma continues to derive guidelines from adult melanoma treatment. However, more drug trials are being conducted to determine the specific impact of drug combinations on pediatric patients. Alongside medical and surgical treatment, prevention is a central component of battling the incidence, as ultraviolet (UV-related mutations play a central role in the vast majority of pediatric melanoma cases. Aggressive prevention measures targeting sun safety and tanning bed usage have shown positive sun-safety behavior trends, as well as the potential to decrease melanomas that manifest later in life. As research into the field of pediatric melanoma continues to expand, a prevention paradigm needs to continue on a community-wide level. Keywords: melanoma, pediatric, adolescent, childhood

  12. Neuropathology and Neurochemistry of Nonmotor Symptoms in Parkinson's Disease

    Directory of Open Access Journals (Sweden)

    Isidro Ferrer

    2011-01-01

    Full Text Available Parkinson disease (PD is no longer considered a complex motor disorder characterized by Parkinsonism but rather a systemic disease with variegated non-motor deficits and neurological symptoms, including impaired olfaction, autonomic failure, cognitive impairment, and psychiatric symptoms. Many of these alterations appear before or in parallel with motor deficits and then worsen with disease progression. Although there is a close relation between motor symptoms and the presence of Lewy bodies (LBs and neurites filled with abnormal -synuclein, other neurological alterations are independent of the amount of -synuclein inclusions in neurons and neurites, thereby indicating that different mechanisms probably converge in the degenerative process. Involvement of the cerebral cortex that may lead to altered behaviour and cognition are related to several convergent factors such as (a abnormal -synuclein and other proteins at the synapses, rather than LBs and neurites, (b impaired dopaminergic, noradrenergic, cholinergic and serotoninergic cortical innervation, and (c altered neuronal function resulting from reduced energy production and increased energy demands. These alterations appear at early stages of the disease and may precede by years the appearance of cell loss and cortical atrophy.

  13. A core handling device for the Mars Sample Return Mission

    Science.gov (United States)

    Gwynne, Owen

    1989-01-01

    A core handling device for use on Mars is being designed. To provide a context for the design study, it was assumed that a Mars Rover/Sample Return (MRSR) Mission would have the following characteristics: a year or more in length; visits by the rover to 50 or more sites; 100 or more meter-long cores being drilled by the rover; and the capability of returning about 5 kg of Mars regolith to Earth. These characteristics lead to the belief that in order to bring back a variegated set of samples that can address the range of scientific objetives for a MRSR mission to Mars there needs to be considerable analysis done on board the rover. Furthermore, the discrepancy between the amount of sample gathered and the amount to be returned suggests that there needs to be some method of choosing the optimal set of samples. This type of analysis will require pristine material-unaltered by the drilling process. Since the core drill thermally and mechanically alters the outer diameter (about 10 pct) of the core sample, this outer area cannot be used. The primary function of the core handling device is to extract subsamples from the core and to position these subsamples, and the core itself if needed, with respect to the various analytical instruments that can be used to perform these analyses.

  14. Underground gas storage Uelsen: Findings from planning, building and commissioning. Part 1: Deposit; Untertagegasspeicher Uelsen: Erkenntnisse aus Planung, Bau und Inbetriebnahme. Teil 1: Lagerstaette

    Energy Technology Data Exchange (ETDEWEB)

    Wallbrecht, J.; Beckmann, H.; Reiser, H.; Wilhelm, R. [BEB Erdgas und Erdoel GmbH, Hannover (Germany)

    1998-12-31

    The underground gas storage at Uelsen which was built as a H-gas storage in a former variegated sandstone gasfield in Western Lower Saxony close to the town of Nordhorn has added to the gas supply system of the BEB Erdgas and Erdoel GmbH. The underground storage is connected to the Bunde-Rheine transport pipeline BEB-grid gas system by a 27 km pipeline and is a consequent expansion of BEB`s underground storage/transport system. Planning, building and commissioning were handled by BEB. Findings to date are described. [Deutsch] Der Untertagegasspeicher (UGS) Uelsen, der in einem ehemaligen Buntsandstein Gasfeld im westlichen Niedersachsen in der Naehe der Stadt Nordhorn als H-Gasspeicher eingerichtet wurde, hat die BEB Erdgas und Erdoel GmbH eine weitere Staerkung ihres Gasversorgungssystems erreicht. Der UGS Uelsen ist ueber eine 27 km lange Anbindungsleitung mit der zum BEB - Ferngasleitungssystems gehoerenden Bunde-Rheine Transportleitung verbunden und stellt eine konsequente Erweiterung des BEB Untertagegasspeicher-/Transportsystems dar. Planung, Bau und Inbetriebnahme erfolgten durch BEB im Rahmen einer integrierten bereichsuebergreifenden Projektbearbeitung. Die hierbei gewonnenen Erkenntnisse werden im Folgenden fuer den Untertagebereich dargestellt. (orig.)

  15. Nephroprotective effect of Bauhinia variegata (Linn.) whole stem extract against cisplatin-induced nephropathy in rats

    Science.gov (United States)

    Pani, Saumya R.; Mishra, Satyaranjan; Sahoo, Sabuj; Panda, Prasana K.

    2011-01-01

    The nephroprotective activity of the ethanolic extract of Bauhinia variegata (Linn.) whole stem against cisplatin-induced nephropathy was investigated by an in vivo method in rats. Acute nephrotoxicity was induced by i.p. injection of cisplatin (7 mg/kg of body weight (b.w.)). Administration of ethanol extract at dose levels of 400 and 200 mg/kg (b.w.) to cisplatin-intoxicated rats for 14 days attenuated the biochemical and histological signs of nephrotoxicity of cisplatin in a dose-dependent fashion. Ethanol extract at 400 mg/kg decreased the serum level of creatinine (0.65 ± 0.09; P<0.001) and urea (32.86 ± 5.88; P<0.001) associated with a significant increase in body weight (7.16 ± 1.10; P<0.001) and urine volume output (11.95 ± 0.79; P<0.05) as compared to the toxic control group. The ethanol extract of B. variegata at 400 mg/kg (b.w.) exhibited significant and comparable nephroprotective potential to that of the standard polyherbal drug cystone. The statistically (one-way-ANOVA followed by Tukey-Kramer multiple comparison) processed results suggested the protective action of B. variegate whole stem against cisplatin-induced nephropathy. PMID:21572659

  16. Micropropagation of Codiaeum variegatum (L.) Blume and regeneration induction via adventitious buds and somatic embryogenesis.

    Science.gov (United States)

    Radice, Silvia

    2010-01-01

    Codiaeum variegatum (L) Blume cv. "Corazon de oro" and cv. "Norma" are successfully micropropagated when culture are initiated with explants taken from newly sprouted shoots. The establishment and multiplication steps are possible when 1 mg/L BA or 1 mg/L IAA and 3 mg/L 2iP are added to MS medium, according to the cultivar respectively selected.Adventive organogenesis and somatic embryogenesis are induced from leaf explants taken from in vitro buds of croton. On leaf-sectioned of "Corazon de oro" cultured in vitro, 1 mg/L BA stimulates continuous somatic embryos development and induces some shoots too. Replacing BA with 1 mg/L TDZ induces up to 100% bud regeneration in the same explants. On the other hand, leaf-sectioned of C. variegatum cv. Norma does not start somatic embryo differentiation if 1 mg/L TDZ is not added to the MS basal medium. Incipient callus is observed after 30 days of culture, and then, subculture to MS with 1 mg/L BA allows the same process to show on the "Corazon de oro" cultivar. Somatic embryos show growth arrest that is partially overcome by transfer to hormone-free basal medium with activated charcoal. Root induction is possible on basal medium plus 1 mg/L IBA. Plantlets in the greenhouse have variegated leaves true-to-type.

  17. Clinical analysis of cases with nasopharyngeal carcinoma

    International Nuclear Information System (INIS)

    Sato, Katsuro; Tomita, Masahiko; Takahashi, Sugata; Matsuyama, Hiroshi

    2010-01-01

    Forty-one cases with nasopharyngeal carcinoma (NPC) treated in our department between 1991 and 2007 were clinically analyzed. The mean age of the cases was 53 years old, and the male-to female ratio was 3.6:1. The most common chief complaint was ear symptoms followed by neck, eye, and nose symptoms. The most common histology was squamous cell carcinoma, followed by undifferentiated carcinoma, adenocarcinoma, and spindle cell carcinoma. More than half of the cases were classified as clinical stage IV. For squamous cell carcinoma, undifferentiated carcinoma, spindle cell carcinoma cases, concurrent chemoradiotherapy followed by adjuvant chemotherapy was applied. For adenocarcinoma cases, transpalatal resection and postoperative radiotherapy was applied. The five-year overall survival rate was 64.1% and the disease-specific five-year survival rate was 71.2%. No significant statistical differences were seen between early stage (I, II) and late stage (III, IV), between I, II, III stage and IV stage. Recurrence occurred in 24.4% of the cases, and distant metastasis was more dominant than local recurrence. For the diagnosis and treatment of NPC, proper detection of NPC from variegated symptoms, and chemoradiotherapy for squamous cell carcinoma cases were considered to be important. (author)

  18. A comparison of social and spatial determinants of health between formal and informal settlements in a large metropolitan setting in Brazil.

    Science.gov (United States)

    Snyder, Robert E; Jaimes, Guillermo; Riley, Lee W; Faerstein, Eduardo; Corburn, Jason

    2014-06-01

    Urban informal settlements are often under-recognized in national and regional surveys. A lack of quality intra-urban data frequently contributes to a one-size-fits-all public health intervention and clinical strategies that rarely address the variegated socioeconomic disparities across and within different informal settlements in a city. The 2010 Brazilian census gathered detailed population and place-based data across the country's informal settlements. Here, we examined key socio-demographic and infrastructure characteristics that are associated with health outcomes in Rio de Janeiro with the census tract as the unit of analysis. Many of the city's residents (1.39 million people, 22 % of the population) live in informal settlements. Residents of census tracts in Rio de Janeiro's urban informal areas are younger, (median age of 26 versus 35 years in formal settlements), and have less access to adequate water (96 versus 99 % of informal households), sanitation (86 versus 96 %), and electricity (67 versus 92 %). Average per household income in informal settlement census tracts is less than one third that of non-informal tracts (US\\$708 versus US\\$2362). Even among informal settlements in different planning areas in the same city, there is marked variation in these characteristics. Public health interventions, clinical management, and urban planning policies aiming to improve the living conditions of the people residing in informal settlements, including government strategies currently underway, must consider the differences that exist between and within informal settlements that shape place-based physical and social determinants of health.

  19. Evaluation of response to immune checkpoint inhibitors: Is there a role for positron emission tomography?

    Institute of Scientific and Technical Information of China (English)

    Matteo Bauckneht; Roberta Piva; Gianmario Sambuceti; Francesco Grossi; Silvia Morbelli

    2017-01-01

    Strategies targeting intracellular negative regulators such as immune checkpoint inhibitors(ICPIs) have demonstrated significant antitumor activity across a wide range of solid tumors. In the clinical practice, the radiological effect of immunotherapeutic agents has raised several more relevant and complex challenges for the determination of their imaging-based response at single patient level. Accordingly, it has been suggested that the conventional Response Evaluation Criteria in Solid Tumors assessment alone, based on dimensional evaluation provided by computed tomography(CT), tends to underestimate the benefit of ICPIs at least in a subset of patients, supporting the need of immunerelated response criteria. Different from CT, very few data are available for the evaluation of immunotherapy by means of 18F-fluoro-2-deoxy-D-glucose positron emission tomography(FDG-PET). Moreover, since the antineoplastic activity of ICPIs is highly related to the activation of T cells against cancer cells, FDG accumulation might cause false-positive findings. Yet, discrimination between benign and malignant processes represents a huge challenge for FDG-PET in this clinical setting. Consequently, it might be of high interest to test the complex and variegated response to ICPIs by means of PET and thus it is worthwhile to ask if a similar introduction of immune-related PET-based criteria could be proposed in the future. Finally, PET might offer a new insight into the biology and pathophysiology of ICPIs thanks to a growing number of non-invasive immunediagnostic approaches based on non-FDG tracers.

  20. The MqsRA Toxin-Antitoxin System from Xylella fastidiosa Plays a Key Role in Bacterial Fitness, Pathogenicity, and Persister Cell Formation

    Science.gov (United States)

    Merfa, Marcus V.; Niza, Bárbara; Takita, Marco A.; De Souza, Alessandra A.

    2016-01-01

    Through the formation of persister cells, bacteria exhibit tolerance to multidrug and other environmental stresses without undergoing genetic changes. The toxin-antitoxin (TA) systems are involved in the formation of persister cells because they are able to induce cell dormancy. Among the TA systems, the MqsRA system has been observed to be highly induced in persister cells of Xylella fastidiosa (causal agent of citrus variegated chlorosis—CVC) activated by copper stress, and has been described in Escherichia coli as related to the formation of persister cells and biofilms. Thus, we evaluated the role of this TA system in X. fastidiosa by overexpressing the MqsR toxin, and verified that the toxin positively regulated biofilm formation and negatively cell movement, resulting in reduced pathogenicity in citrus plants. The overexpression of MqsR also increased the formation of persister cells under copper stress. Analysis of the gene and protein expression showed that this system likely has an autoregulation mechanism to express the toxin and antitoxin in the most beneficial ratio for the cell to oppose stress. Our results suggest that this TA system plays a key role in the adaptation and survival of X. fastidiosa and reveal new insights into the physiology of phytopathogen-host interactions. PMID:27375608

  1. VapD in Xylella fastidiosa Is a Thermostable Protein with Ribonuclease Activity.

    Science.gov (United States)

    Mendes, Juliano S; Santiago, André da S; Toledo, Marcelo A S; Rosselli-Murai, Luciana K; Favaro, Marianna T P; Santos, Clelton A; Horta, Maria Augusta C; Crucello, Aline; Beloti, Lilian L; Romero, Fabian; Tasic, Ljubica; de Souza, Alessandra A; de Souza, Anete P

    2015-01-01

    Xylella fastidiosa strain 9a5c is a gram-negative phytopathogen that is the causal agent of citrus variegated chlorosis (CVC), a disease that is responsible for economic losses in Brazilian agriculture. The most well-known mechanism of pathogenicity for this bacterial pathogen is xylem vessel occlusion, which results from bacterial movement and the formation of biofilms. The molecular mechanisms underlying the virulence caused by biofilm formation are unknown. Here, we provide evidence showing that virulence-associated protein D in X. fastidiosa (Xf-VapD) is a thermostable protein with ribonuclease activity. Moreover, protein expression analyses in two X. fastidiosa strains, including virulent (Xf9a5c) and nonpathogenic (XfJ1a12) strains, showed that Xf-VapD was expressed during all phases of development in both strains and that increased expression was observed in Xf9a5c during biofilm growth. This study is an important step toward characterizing and improving our understanding of the biological significance of Xf-VapD and its potential functions in the CVC pathosystem.

  2. In vitro Determination of Extracellular Proteins from Xylella fastidiosa.

    Science.gov (United States)

    Mendes, Juliano S; Santiago, André S; Toledo, Marcelo A S; Horta, Maria A C; de Souza, Alessandra A; Tasic, Ljubica; de Souza, Anete P

    2016-01-01

    The phytopathogen Xylella fastidiosa causes economic losses in important agricultural crops. Xylem vessel occlusion caused by biofilm formation is the major mechanism underlying the pathogenicity of distinct strains of X. fastidiosa . Here, we provide a detailed in vitro characterization of the extracellular proteins of X. fastidiosa . Based on the results, we performed a comparison with a strain J1a12, which cannot induce citrus variegated chlorosis symptoms when inoculated into citrus plants. We then extend this approach to analyze the extracellular proteins of X. fastidiosa in media supplemented with calcium. We verified increases in extracellular proteins concomitant with the days of growth and, consequently, biofilm development (3-30 days). Outer membrane vesicles carrying toxins were identified beginning at 10 days of growth in the 9a5c strain. In addition, a decrease in extracellular proteins in media supplemented with calcium was observed in both strains. Using mass spectrometry, 71 different proteins were identified during 30 days of X. fastidiosa biofilm development, including proteases, quorum-sensing proteins, biofilm formation proteins, hypothetical proteins, phage-related proteins, chaperones, toxins, antitoxins, and extracellular vesicle membrane components.

  3. Comparative genomic analysis of coffee-infecting Xylella fastidiosa strains isolated from Brazil.

    Science.gov (United States)

    Barbosa, Deibs; Alencar, Valquíria Campos; Santos, Daiene Souza; de Freitas Oliveira, Ana Cláudia; de Souza, Alessandra A; Coletta-Filho, Helvecio D; de Oliveira, Regina Souza; Nunes, Luiz R

    2015-05-01

    Strains of Xylella fastidiosa constitute a complex group of bacteria that develop within the xylem of many plant hosts, causing diseases of significant economic importance, such as Pierce's disease in North American grapevines and citrus variegated chlorosis in Brazil. X. fastidiosa has also been obtained from other host plants, in direct correlation with the development of diseases, as in the case of coffee leaf scorch (CLS)--a disease with potential to cause severe economic losses to the Brazilian coffee industry. This paper describes a thorough genomic characterization of coffee-infecting X. fastidiosa strains, initially performed through a microarray-based approach, which demonstrated that CLS strains could be subdivided in two phylogenetically distinct subgroups. Whole-genomic sequencing of two of these bacteria (one from each subgroup) allowed identification of ORFs and horizontally transferred elements (HTEs) that were specific to CLS-related X. fastidiosa strains. Such analyses confirmed the size and importance of HTEs as major mediators of chromosomal evolution amongst these bacteria, and allowed identification of differences in gene content, after comparisons were made with previously sequenced X. fastidiosa strains, isolated from alternative hosts. Although direct experimentation still needs to be performed to elucidate the biological consequences associated with such differences, it was interesting to verify that CLS-related bacteria display variations in genes that produce toxins, as well as surface-related factors (such as fimbrial adhesins and LPS) that have been shown to be involved with recognition of specific host factors in different pathogenic bacteria. © 2015 The Authors.

  4. The diversity of citrus endophytic bacteria and their interactions with Xylella fastidiosa and host plants

    Science.gov (United States)

    Azevedo, João Lúcio; Araújo, Welington Luiz; Lacava, Paulo Teixeira

    2016-01-01

    Abstract The bacterium Xylella fastidiosa is the causal agent of citrus variegated chlorosis (CVC) and has been associated with important losses in commercial orchards of all sweet orange [Citrus sinensis (L.)] cultivars. The development of this disease depends on the environmental conditions, including the endophytic microbial community associated with the host plant. Previous studies have shown that X. fastidiosa interacts with the endophytic community in xylem vessels as well as in the insect vector, resulting in a lower bacterial population and reduced CVC symptoms. The citrus endophytic bacterium Methylobacterium mesophilicum can trigger X. fastidiosa response in vitro, which results in reduced growth and induction of genes associated with energy production, stress, transport, and motility, indicating that X. fastidiosa has an adaptive response to M. mesophilicum. Although this response may result in reduced CVC symptoms, the colonization rate of the endophytic bacteria should be considered in studies that intend to use this endophyte to suppress CVC disease. Symbiotic control is a new strategy that uses symbiotic endophytes as biological control agents to antagonize or displace pathogens. Candidate endophytes for symbiotic control of CVC must occupy the xylem of host plants and attach to the precibarium of sharpshooter insects to access the pathogen. In the present review, we focus on interactions between endophytic bacteria from sweet orange plants and X. fastidiosa, especially those that may be candidates for control of CVC. PMID:27727362

  5. Differential gene expression in Xylella fastidiosa 9a5c during co-cultivation with the endophytic bacterium Methylobacterium mesophilicum SR1.6/6.

    Science.gov (United States)

    Dourado, Manuella Nóbrega; Santos, Daiene Souza; Nunes, Luiz Roberto; Costa de Oliveira, Regina Lúcia Batista da; de Oliveira, Marcus Vinicius; Araújo, Welington Luiz

    2015-12-01

    Xylella fastidiosa, the causal agent of citrus variegated chlorosis (CVC), colonizes plant xylem, reducing sap flow, and inducing internerval chlorosis, leaf size reduction, necrosis, and harder and smaller fruits. This bacterium may be transmitted from plant to plant by sharpshooter insects, including Bucephalogonia xanthopis. The citrus endophytic bacterium Methylobacterium mesophilicum SR1.6/6 colonizes citrus xylem and previous studies showed that this strain is also transferred from plant to plant by B. xanthopis (Insecta), suggesting that this endophytic bacterium may interact with X. fastidiosa in planta and inside the insect vector during co-transmission by the same insect vector. To better understand the X. fastidiosa behavior in the presence of M. mesophilicum, we evaluated the X. fastidiosa transcriptional profile during in vitro interaction with M. mesophilicum SR1.6/6. The results showed that during co-cultivation, X. fastidiosa down-regulated genes related to growth and up-regulated genes related to energy production, stress, transport, and motility, suggesting the existence of a specific adaptive response to the presence of M. mesophilicum in the culture medium. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  6. Functional and Evolutionary Characterization of a UDP-Xylose Synthase Gene from the Plant Pathogen Xylella fastidiosa, Involved in the Synthesis of Bacterial Lipopolysaccharide.

    Science.gov (United States)

    Alencar, Valquíria Campos; Jabes, Daniela Leite; Menegidio, Fabiano Bezerra; Sassaki, Guilherme Lanzi; de Souza, Lucas Rodrigo; Puzer, Luciano; Meneghetti, Maria Cecília Zorél; Lima, Marcelo Andrade; Tersariol, Ivarne Luis Dos Santos; de Oliveira, Regina Costa; Nunes, Luiz R

    2017-02-07

    Xylella fastidiosa is a plant-infecting bacillus, responsible for many important crop diseases, such as Pierce's disease of vineyards, citrus variegated chlorosis, and coffee leaf scorch (CLS), among others. Recent genomic comparisons involving two CLS-related strains, belonging to X. fastidiosa subsp. pauca, revealed that one of them carries a frameshift mutation that inactivates a gene encoding an oxidoreductase of the short-chain dehydrogenase/reductase (SDR) superfamily, which may play important roles in determining structural variations in bacterial glycans and glycoconjugates. However, the exact nature of this SDR has been a matter of controversy, as different annotations of X. fastidiosa genomes have implicated it in distinct reactions. To confirm the nature of this mutated SDR, a comparative analysis was initially performed, suggesting that it belongs to a subgroup of SDR decarboxylases, representing a UDP-xylose synthase (Uxs). Functional assays, using a recombinant derivative of this enzyme, confirmed its nature as XfUxs, and carbohydrate composition analyses, performed with lipopolysaccharide (LPS) molecules obtained from different strains, indicate that inactivation of the X. fastidiosa uxs gene affects the LPS structure among CLS-related X. fastidiosa strains. Finally, a comparative sequence analysis suggests that this mutation is likely to result in a morphological and evolutionary hallmark that differentiates two subgroups of CLS-related strains, which may influence interactions between these bacteria and their plant and/or insect hosts.

  7. A Simple Defined Medium for the Production of True Diketopiperazines in Xylella fastidiosa and Their Identification by Ultra-Fast Liquid Chromatography-Electrospray Ionization Ion Trap Mass Spectrometry.

    Science.gov (United States)

    Silva, Michelli Massaroli da; Andrade, Moacir Dos Santos; Bauermeister, Anelize; Merfa, Marcus Vinícius; Forim, Moacir Rossi; Fernandes, João Batista; Vieira, Paulo Cezar; Silva, Maria Fátima das Graças Fernandes da; Lopes, Norberto Peporine; Machado, Marcos Antônio; Souza, Alessandra Alves de

    2017-06-13

    Diketopiperazines can be generated by non-enzymatic cyclization of linear dipeptides at extreme temperature or pH, and the complex medium used to culture bacteria and fungi including phytone peptone and trypticase peptone, can also produce cyclic peptides by heat sterilization. As a result, it is not always clear if many diketopiperazines reported in the literature are artifacts formed by the different complex media used in microorganism growth. An ideal method for analysis of these compounds should identify whether they are either synthesized de novo from the products of primary metabolism and deliver true diketopiperazines. A simple defined medium ( X. fastidiosa medium or XFM) containing a single carbon source and no preformed amino acids has emerged as a method with a particularly high potential for the grown of X. fastidiosa and to produce genuine natural products. In this work, we identified a range of diketopiperazines from X. fastidiosa 9a5c growth in XFM, using Ultra-Fast Liquid Chromatography coupled with mass spectrometry. Diketopiperazines are reported for the first time from X. fastidiosa , which is responsible for citrus variegated chlorosis. We also report here fatty acids from X. fastidiosa , which were not biologically active as diffusible signals, and the role of diketopiperazines in signal transduction still remains unknown.

  8. The complex biogeography of the plant pathogen Xylella fastidiosa: genetic evidence of introductions and Subspecific introgression in Central America.

    Science.gov (United States)

    Nunney, Leonard; Ortiz, Beatriz; Russell, Stephanie A; Ruiz Sánchez, Rebeca; Stouthamer, Richard

    2014-01-01

    The bacterium Xylella fastidiosa is a plant pathogen with a history of economically damaging introductions of subspecies to regions where its other subspecies are native. Genetic evidence is presented demonstrating the introduction of two new taxa into Central America and their introgression into the native subspecies, X. fastidiosa subsp. fastidiosa. The data are from 10 genetic outliers detected by multilocus sequence typing (MLST) of isolates from Costa Rica. Six (five from oleander, one from coffee) defined a new sequence type (ST53) that carried alleles at six of the eight loci sequenced (five of the seven MLST loci) diagnostic of the South American subspecies Xylella fastidiosa subsp. pauca which causes two economically damaging plant diseases, citrus variegated chlorosis and coffee leaf scorch. The two remaining loci of ST53 carried alleles from what appears to be a new South American form of X. fastidiosa. Four isolates, classified as X. fastidiosa subsp. fastidiosa, showed a low level of introgression of non-native DNA. One grapevine isolate showed introgression of an allele from X. fastidiosa subsp. pauca while the other three (from citrus and coffee) showed introgression of an allele with similar ancestry to the alleles of unknown origin in ST53. The presence of X. fastidiosa subsp. pauca in Central America is troubling given its disease potential, and establishes another route for the introduction of this economically damaging subspecies into the US or elsewhere, a threat potentially compounded by the presence of a previously unknown form of X. fastidiosa.

  9. RNA-Seq analysis of Citrus reticulata in the early stages of Xylella fastidiosa infection reveals auxin-related genes as a defense response.

    Science.gov (United States)

    Rodrigues, Carolina M; de Souza, Alessandra A; Takita, Marco A; Kishi, Luciano T; Machado, Marcos A

    2013-10-03

    Citrus variegated chlorosis (CVC), caused by Xylella fastidiosa, is one the most important citrus diseases, and affects all varieties of sweet orange (Citrus sinensis L. Osb). On the other hand, among the Citrus genus there are different sources of resistance against X. fastidiosa. For these species identifying these defense genes could be an important step towards obtaining sweet orange resistant varieties through breeding or genetic engineering. To assess these genes we made use of mandarin (C. reticulata Blanco) that is known to be resistant to CVC and shares agronomical characteristics with sweet orange. Thus, we investigated the gene expression in Ponkan mandarin at one day after infection with X. fastidiosa, using RNA-seq. A set of genes considered key elements in the resistance was used to confirm its regulation in mandarin compared with the susceptible sweet orange. Gene expression analysis of mock inoculated and infected tissues of Ponkan mandarin identified 667 transcripts repressed and 724 significantly induced in the later. Among the induced transcripts, we identified genes encoding proteins similar to Pattern Recognition Receptors. Furthermore, many genes involved in secondary metabolism, biosynthesis and cell wall modification were upregulated as well as in synthesis of abscisic acid, jasmonic acid and auxin. This work demonstrated that the defense response to the perception of bacteria involves cell wall modification and activation of hormone pathways, which probably lead to the induction of other defense-related genes. We also hypothesized the induction of auxin-related genes indicates that resistant plants initially recognize X. fastidiosa as a necrotrophic pathogen.

  10. "Real-life" treatment of chronic pain: Targets and goals.

    Science.gov (United States)

    Ablin, Jacob N; Buskila, Dan

    2015-02-01

    Treating chronic pain is a complex challenge. While textbooks and medical education classically categorize pain as originating from peripheral (nociceptive), neuropathic, or centralized origins, in real life each and every patient may present a combination of various pain sources, types, and mechanisms. Moreover, individual patients may evolve and develop differing types of pain throughout their clinical follow-up, further emphasizing the necessity to maintain clinical diligence during the evaluation and follow-up of these patients. Rational treatment of patients suffering from chronic pain must attempt at deconstructing complex pain cases, identifying variegate pain generators, and targeting them with appropriate interventions, while incorporating both pharmacological and non-pharmacological strategies, rather than focusing on the total pain level, which represents an integral of all pain types. Failing to recognize the coexistence of different types of pain in an individual patient and escalating medications only on the basis of total pain intensity are liable to lead to both ineffective control of pain and increased untoward effects. In the current review, we outline strategies for deconstructing complex pain and therapeutic suggestions. Copyright © 2015 Elsevier Ltd. All rights reserved.

  11. Lithology and uranium potential of Jurassic formations in the San Ysidro--Cuba and majors ranch areas, northwestern New Mexico

    International Nuclear Information System (INIS)

    Santos, E.S.

    1975-01-01

    The aggregate thickness of sedimentary rocks of Jurassic age near the eastern and southeastern margin of the San Juan Basin in Sandoval County, N. Mex., is about 1150 feet (350 metres). The Entrada Sandstone is the base. The Entrada Sandstone, 97 to 227 feet (30 to 69 m) thick, consists of red and brown siltstone and fine-grained sandstone and brown and white sandstone. The Todilto Formation, 5 to 125 feet (1.5 to 38 m) thick, consists of a limestone unit and a massive white gypsum unit. The Summerville Formation, 0 to 50 feet (0 to 15 m) thick, consists of variegated, interstratified mudstone, claystone, siltstone, and sandstone. The Morrison Formation, 750 to 870 feet (229 to 265 m) thick, is divided into three members. The Recapture Member consists mainly of red and white color-banded fine-grained sandstone. The Westwater Canyon and Brushy Basin Members consist mainly of red and green mudstone interstratified with grayish-orange arkosic sandstone. The upper unit of the Brushy Basin Member is called the Jackpile sandstone, a name of economic usage. Most of the sandstone in the Morrison Formation above the Recapture Member in the area studied is considered to be a potential host for uranium ore deposits. (auth)

  12. Prospects of biomass energy in Bangladesh: an alternative development

    International Nuclear Information System (INIS)

    Salahuddin, Ahmed

    1998-01-01

    Biomass plays an important and complex role in the lives of the people of rural Bangladesh, where more than 80 per cent of the country's population live. The problems relating to biomass do not have to do merely with the question of supply of wood, or of food or of fuel; the problems are linked to competition in the variegations of land-use and to differing end-uses of by-products that may compete with or complement each other. The paper discusses the present pattern and amount of biomass consumption with a view to assessing the future prospect of biomass supply in meeting various needs. Regarding biomass energy supply, several important conclusions can be drawn: a) the energy consumption pattern in Bangladesh is characterized by heavy dependence on traditional fuel; b) the domestic sector uses 80 per cent of the total biomass fuel and c) in the industrial sector, about 76 per cent of the energy used consists of biomass fuel, mainly for processing agricultural products. Several observations are made pertaining to different sectors of biomass fuel demand. (author)

  13. Gamma Irradiation on Growth and Development of Amorphophallus muelleri Blume.

    Directory of Open Access Journals (Sweden)

    Edi Santosa

    2014-09-01

    Full Text Available ABSTRACT Iles-iles (Amorphophallus muelleri Blume produces apomictic seeds lead to low genetic variation. In order to induce genetic variation, germinated seeds were exposed to Gamma irradiation (Co-60 at doses of 10 to 100 Gy. Seed irradiation was conducted at Center for the Application of Isotope and Irradiation Technology -National Nuclear Energy Agency (CAIRT, Indonesia. Morphology and yield of M1 generation were observed. Results showed that irradiation at a dose of 10 Gy close to LD50 with survival rate 56%. Gamma irradiation at a dose of 10 Gy delayed seeds germination.  Germination rates gradually increased and reached maximum at 4 weeks after planting (WAP for control plants, and 14 WAP of irradiated plants. At 16 WAP, germination rate of 10 Gy irradiated plants was 56% and 84% for those of control plants. Irradiation induced chimera as indicated by short petiole, variegated and abnornal shape of leaflets. Some irradiated plants entered dormancy at 8-10 weeks later than control ones. Prolong vegetative periode lead the plants to produce heavier corms. This study revealed the possibility to induce variation of A. muelleri by using gamma irradition. Keywords: Amorphophallus muelleri, gamma irradiation (Co-60, morphological variation, mutation breeding

  14. The radioisotopically constrained Viséan onset of turbidites in the Moravian-Silesian part of the Rhenohercynian foreland basin (Central European Variscides)

    Science.gov (United States)

    Jirásek, Jakub; Otava, Jiří; Matýsek, Dalibor; Sivek, Martin; Schmitz, Mark D.

    2018-03-01

    The Březina Formation represents the initiation of siliciclastic flysch turbidite sedimentation at the eastern margin of Bohemian Massif or within the Rhenohercynian foreland basin. Its deposition started after drowning of the Devonian carbonate platform during Viséan (Mississippian) times, resulting in a significant interval of black siliceous shale and variegated fossiliferous shale deposition in a starved basin. Near the top of the Březina Formation an acidic volcanoclastic layer (tuff) of rhyolitic composition has been dated with high precision U-Pb zircon chemical abrasion isotope dilution method at 337.73 ± 0.16 Ma. This new radiometric age correlates with the previously inferred stratigraphic age of the locality and the current calibration of the Early Carboniferous geologic time scale. Shales of the Březina Formation pass gradually upwards into the siliciclastics of the Rozstání Formation of the Drahany culm facies. Thus our new age offers one of the few available radioisotopic constraints on the time of onset of siliciclastic flysch turbidites in the Rhenohercynian foreland basin of the European Variscides.

  15. Gondwana basins and their coal resources in Bangladesh

    International Nuclear Information System (INIS)

    Nehaluddin, M.; Sultan-ul-Islam, M.

    1994-01-01

    Fault bounded five Gondwana basins have been discovered in the north western Bangladesh. Among these basins show considerable amount of coal deposits. The Gondwana rocks are highly formed during the Permo-carboniferous diastrophism and later on acquired dynamic characters. In almost all basins, the Permian rocks overlie the Precambrian basement and underlie either the Tertiary or the Cretaceous sediments, structural, stratigraphic, and depositional history of these basins is more or less similar. The sedimentary sequences are composed of light to dark gray, fine to very coarse grained, sub angular to sub rounded felspathic sandstone, dark grey carbonaceous shale and sandstone, variegated conglomerate and thick coal seams (single seam max. 42.38m). The rocks are often alternated and bear the characteristics of cyclic sedimentation. The depositional environments varied from restricted drainage to open fluvial dominated low to moderate sinuous drainage system. The coal bearing basins were flanked by vegetated and swampy over bank. Age of these coals is suggested to be the late permian. Proved and probable reserves of coal in Jamalganj-Paharpur basin are 670 and 1,460 million metric tons, in Barapukuria basin 303 and 3899 million metric tons; in Barapukuria basin 303 and 389 million metric tons; and in Khalaspir basin 143 and 685 million metric tons respectively. The coal is high volatile, low sulphur, bituminous type. It can be used for different forms of thermal conversion. (author)

  16. TALEN-mediated single-base-pair editing identification of an intergenic mutation upstream of BUB1B as causative of PCS (MVA) syndrome

    Science.gov (United States)

    Ochiai, Hiroshi; Miyamoto, Tatsuo; Kanai, Akinori; Hosoba, Kosuke; Sakuma, Tetsushi; Kudo, Yoshiki; Asami, Keiko; Ogawa, Atsushi; Watanabe, Akihiro; Kajii, Tadashi; Yamamoto, Takashi; Matsuura, Shinya

    2014-01-01

    Cancer-prone syndrome of premature chromatid separation with mosaic variegated aneuploidy [PCS (MVA) syndrome] is a rare autosomal recessive disorder characterized by constitutional aneuploidy and a high risk of childhood cancer. We previously reported monoallelic mutations in the BUB1B gene (encoding BUBR1) in seven Japanese families with the syndrome. No second mutation was found in the opposite allele of any of the families studied, although a conserved BUB1B haplotype and a decreased transcript were identified. To clarify the molecular pathology of the second allele, we extended our mutational search to a candidate region surrounding BUB1B. A unique single nucleotide substitution, G > A at ss802470619, was identified in an intergenic region 44 kb upstream of a BUB1B transcription start site, which cosegregated with the disorder. To examine whether this is the causal mutation, we designed a transcription activator-like effector nuclease–mediated two-step single-base pair editing strategy and biallelically introduced this substitution into cultured human cells. The cell clones showed reduced BUB1B transcripts, increased PCS frequency, and MVA, which are the hallmarks of the syndrome. We also encountered a case of a Japanese infant with PCS (MVA) syndrome carrying a homozygous single nucleotide substitution at ss802470619. These results suggested that the nucleotide substitution identified was the causal mutation of PCS (MVA) syndrome. PMID:24344301

  17. Ticks on captive and free-living wild animals in northeastern Brazil.

    Science.gov (United States)

    Dantas-Torres, Filipe; Ferreira, Débora R A; de Melo, Louise M; Lima, Polly-Ana C P; Siqueira, Daniel B; Rameh-de-Albuquerque, Luciana C; de Melo, Adriana V; Ramos, Janaina A C

    2010-02-01

    From 2005 to 2009, 147 ticks found on 32 wild animals from or referred to two zoobotanical parks (Parque Zoobotânico Arruda Câmara and Parque Estadual Dois Irmãos) located in northeastern Brazil were identified. Ticks found on two veterinarians working in one of the parks (i.e., Parque Estadual Dois Irmãos), after return from forested areas within the park's territory, were also identified. The following tick-host associations were recorded: Amblyomma fuscum Neumann on Boa constrictor L.; Amblyomma longirostre Koch on Ramphastos vitellinus ariel Vigors and Coendou prehensilis (L.); Amblyomma varium Koch on Bradypus variegates Schinz; Amblyomma rotundatum Koch on Chelonoidis carbonaria (Spix), Chelonoidis denticulata (L.), Micrurus ibiboboca (Merrem), Python molurus bivittatus Kuhl, Iguana iguana (L.) and B. variegatus; Amblyomma nodosum Neumann on Myrmecophaga tridactyla L. and Tamandua tetradactyla (L.); and Rhipicephalus sanguineus (Latreille) on Nasua nasua (L.). The ticks found on the veterinarians were identified as nine Amblyomma larvae. The presence of Am. nodosum in Pernambuco and Am. rotundatum and Am. varium in Paraíba is recorded for the first time and the occurrence of Am. longirostre in Pernambuco is confirmed. Ramphastos vitellinus ariel is a new host record for Am. longirostre whereas M. ibiboboca and B. variegatus are new host records for Am. rotundatum. Finally, the human parasitism by Amblyomma ticks is reported for the first time in Pernambuco, highlighting the potential of tick-borne pathogen transmission in this state.

  18. Human HMG box transcription factor HBP1: a role in hCD2 LCR function.

    Science.gov (United States)

    Zhuma, T; Tyrrell, R; Sekkali, B; Skavdis, G; Saveliev, A; Tolaini, M; Roderick, K; Norton, T; Smerdon, S; Sedgwick, S; Festenstein, R; Kioussis, D

    1999-01-01

    The locus control region (LCR) of the human CD2 gene (hCD2) confers T cell-specific, copy-dependent and position-independent gene expression in transgenic mice. This LCR consists of a strong T cell-specific enhancer and an element without enhancer activity (designated HSS3), which is required for prevention of position effect variegation (PEV) in transgenic mice. Here, we identified the HMG box containing protein-1 (HBP1) as a factor binding to HSS3 of the hCD2 LCR. Within the LCR, HBP1 binds to a novel TTCATTCATTCA sequence that is higher in affinity than other recently reported HBP1-binding sites. Mice transgenic for a hCD2 LCR construct carrying a deletion of the HBP1-binding sequences show a propensity for PEV if the transgene integrates in a heterochromatic region of the chromosome such as the centromere or telomere. We propose that HBP1 plays an important role in chromatin opening and remodelling activities by binding to and bending the DNA, thus allowing DNA-protein and/or protein-protein interactions, which increase the probability of establishing an active locus. PMID:10562551

  19. Induction of mutant resistant to alternaria blotch of apple by gamma-ray irradiation

    International Nuclear Information System (INIS)

    Yoshioka, Toji; Ito, Yuji; Masuda, Tetsuo

    2000-01-01

    Apple cultivars resistant to Alternaria blotch disease have been produced by cross-breeding, but it is difficult to produce resistance by crossing without changing the properties of cultivar because the gene composition of the cultivar tree is almost heterozygous. This study aimed to investigate the resistant mutation in Alternaria blotch susceptible and semiresistant cultivars. The resistance to Alternaria blotch pathogen or AM toxin is classified into the following three groups: 1) highly sensitive group including Indo, Redgold and Starking delicious, 2) semi-resistant group including Fuji, Orin and Golden delicious and 3) resistant group including Gala and Tsugaru. After gamma ray exposure of 80 Gy (at 5 Gy/hour), AM-toxin insensitive clones were selected in the VM 6 generation. These selected mutants could be rooted and habituated under field conditions. The degree of disease resistance was assessed by AM toxin treatment and Alternaria blotch fungi spore inoculation test. The leaves of these mutants were changed to variegated at high temperature, suggesting that some mutation related to chloroplast might have occurred. Alternaria blotch resistant strains could be produced by exposing to γray and selecting with AM toxin in shoot-tip culture system, but the functional effects of the AM toxin in Alternaria blotch and also the mechanism in the mutant lines were still unclear. (M.N.)

  20. Chloroplast Redox Imbalance Governs Phenotypic Plasticity: the Grand Design of Photosynthesis Revisited

    Directory of Open Access Journals (Sweden)

    Norman eHuner

    2012-11-01

    Full Text Available Sunlight, the ultimate energy source for life on our planet, enters the biosphere as a direct consequence of the evolution of photoautotrophy. Photoautotrophs must balance the light energy absorbed and trapped through extremely fast, temperature-insensitive photochemistry with energy consumed through much slower, temperature-dependent biochemistry and metabolism. The attainment of such a balance in cellular energy flow between chloroplasts, mitochondria and the cytosol is called photostasis. Photoautotrophs sense cellular energy imbalances through modulation of excitation pressure which is a measure of the relative redox state of QA, the first stable quinone electron acceptor of PSII reaction centers. High excitation pressure constitutes a potential stress condition that can be caused either by exposure to an irradiance that exceeds the capacity of C, N and S assimilation to utilize the electrons generated from the absorbed energy or by low temperature or any stress that decreases the capacity of the metabolic pathways downstream of photochemistry to utilize photosynthetically-generated reductants. The similarities and differences in the phenotypic responses between cyanobacteria, green algae, crop plants and variegation mutants of Arabidopsis thaliana as a function of cold acclimation and photoacclimation are reconciled in terms of differential responses to excitation pressure and the predisposition of photoautotrophs to maintain photostasis. The various acclimation strategies associated with green algae and cyanobacteria versus winter cereals and Arabidopsis thaliana are discussed in terms of retrograde regulation and the grand design of photosynthesis originally proposed by Daniel Arnon in 1982.

  1. SOT1, a pentatricopeptide repeat protein with a small MutS-related domain, is required for correct processing of plastid 23S-4.5S rRNA precursors in Arabidopsis thaliana.

    Science.gov (United States)

    Wu, Wenjuan; Liu, Sheng; Ruwe, Hannes; Zhang, Delin; Melonek, Joanna; Zhu, Yajuan; Hu, Xupeng; Gusewski, Sandra; Yin, Ping; Small, Ian D; Howell, Katharine A; Huang, Jirong

    2016-03-01

    Ribosomal RNA processing is essential for plastid ribosome biogenesis, but is still poorly understood in higher plants. Here, we show that SUPPRESSOR OF THYLAKOID FORMATION1 (SOT1), a plastid-localized pentatricopeptide repeat (PPR) protein with a small MutS-related domain, is required for maturation of the 23S-4.5S rRNA dicistron. Loss of SOT1 function leads to slower chloroplast development, suppression of leaf variegation, and abnormal 23S and 4.5S processing. Predictions based on the PPR motif sequences identified the 5' end of the 23S-4.5S rRNA dicistronic precursor as a putative SOT1 binding site. This was confirmed by electrophoretic mobility shift assay, and by loss of the abundant small RNA 'footprint' associated with this site in sot1 mutants. We found that more than half of the 23S-4.5S rRNA dicistrons in sot1 mutants contain eroded and/or unprocessed 5' and 3' ends, and that the endonucleolytic cleavage product normally released from the 5' end of the precursor is absent in a sot1 null mutant. We postulate that SOT1 binding protects the 5' extremity of the 23S-4.5S rRNA dicistron from exonucleolytic attack, and favours formation of the RNA structure that allows endonucleolytic processing of its 5' and 3' ends. © 2016 The Authors The Plant Journal © 2016 John Wiley & Sons Ltd.

  2. New pathologic entities in penile carcinomas: an update of the 2004 world health organization classification.

    Science.gov (United States)

    Chaux, Alcides; Velazquez, Elsa F; Barreto, José E; Ayala, Enrique; Cubilla, Antonio L

    2012-05-01

    Most primary malignant tumors of the penis are squamous cell carcinomas (SCC) of the usual type. In recent years several variants, each with distinctive clinicopathologic features, have been described. Pseudohyperplastic carcinoma and carcinoma cuniculatum are both low-grade, extremely well-differentiated SCC variants characterized by an indolent clinical course and good prognosis. The former, which may be confused with pseudoepitheliomatous hyperplasia, preferentially affects the inner foreskin mucosa of elderly men and the latter is a verruciform tumor with an endophytic, burrow-like pattern of growth. Pseudoglandular carcinoma (featuring solid tumor nests with extensive central acantholysis simulating glandular lumina) and clear cell carcinoma (human papillomavirus [HPV]-related tumors composed of periodic acid-Schiff positive clear cells) are aggressive tumors with a high incidence of inguinal nodal metastases. Papillary carcinomas are HPV-unrelated verruciform tumors composed of complex papillae with acanthosis, hyper- and parakeratosis, absence of koilocytes, irregular fibrovascular cores, and jagged tumor base. Finally, in warty-basaloid carcinomas areas of warty (condylomatous) and basaloid carcinomas coexist in the same tumor, either separated or intermingled, giving the tumor a variegated appearance. In this review special emphasis is given to the differential diagnosis of these special variants with a discussion of the possible implications for clinical management.

  3. The diversity of citrus endophytic bacteria and their interactions with Xylella fastidiosa and host plants

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    João Lúcio Azevedo

    Full Text Available Abstract The bacterium Xylella fastidiosa is the causal agent of citrus variegated chlorosis (CVC and has been associated with important losses in commercial orchards of all sweet orange [Citrus sinensis (L.] cultivars. The development of this disease depends on the environmental conditions, including the endophytic microbial community associated with the host plant. Previous studies have shown that X. fastidiosa interacts with the endophytic community in xylem vessels as well as in the insect vector, resulting in a lower bacterial population and reduced CVC symptoms. The citrus endophytic bacterium Methylobacterium mesophilicum can trigger X. fastidiosa response in vitro, which results in reduced growth and induction of genes associated with energy production, stress, transport, and motility, indicating that X. fastidiosa has an adaptive response to M. mesophilicum. Although this response may result in reduced CVC symptoms, the colonization rate of the endophytic bacteria should be considered in studies that intend to use this endophyte to suppress CVC disease. Symbiotic control is a new strategy that uses symbiotic endophytes as biological control agents to antagonize or displace pathogens. Candidate endophytes for symbiotic control of CVC must occupy the xylem of host plants and attach to the precibarium of sharpshooter insects to access the pathogen. In the present review, we focus on interactions between endophytic bacteria from sweet orange plants and X. fastidiosa, especially those that may be candidates for control of CVC.

  4. Moving Encounters

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    Ann Deslandes

    2011-04-01

    Full Text Available This essay explores my experience of losing an authoritative speaking position – that is, ‘falling on my face’ in a research encounter with the Brazilian Landless Worker’s Movement (O Movimento dos Trabalhadores Rurais sem Terra, or MST.  My specific movements through this locale invoke Gayatri Chakravorty Spivak’s concept of ‘unlearning one’s privilege as a loss’; and Sara Ahmed’s theory of ‘stranger fetishism’.  In writing my brief loss, I also; of course, recover my speaking position, meaning that I can always efface the loss by re-writing it as a source of ethnographic authority.  This essay is written in two voices in order to reflect this paradox: one which describes the encounter, and one that critically ruminates upon it.  I note, for example, that the MST as a variegated conglomerate of people takes the form of particular ‘Others’ when they are represented in the scholarship and polemic of ‘first world’ activists in the so called ‘global justice movement’.  ‘Falling on my face in the street’ of these Others locates particular processes of fetishization within the global justice movement and the relationships across power and difference that are contained herein; processes that impact on the idea of a ‘global’ solidarity against systemic ‘global’ oppressions.

  5. mRNA Traffic Control Reviewed: N6-Methyladenosine (m6 A) Takes the Driver's Seat.

    Science.gov (United States)

    Visvanathan, Abhirami; Somasundaram, Kumaravel

    2018-01-01

    Messenger RNA is a flexible tool box that plays a key role in the dynamic regulation of gene expression. RNA modifications variegate the message conveyed by the mRNA. Similar to DNA and histone modifications, mRNA modifications are reversible and play a key role in the regulation of molecular events. Our understanding about the landscape of RNA modifications is still rudimentary in contrast to DNA and histone modifications. The major obstacle has been the lack of sensitive detection methods since they are non-editing events. However, with the advent of next-generation sequencing techniques, RNA modifications are being identified precisely at single nucleotide resolution. In recent years, methylation at the N6 position of adenine (m 6 A) has gained the attention of RNA biologists. The m 6 A modification has a set of writers (methylases), erasers (demethylases), and readers. Here, we provide a summary of interesting facts, conflicting findings, and recent advances in the technical and functional aspects of the m 6 A epitranscriptome. © 2017 WILEY Periodicals, Inc.

  6. Peculiarities of 137Cs accumulation by macromycetes in dry bors of Ukrainian Polessye

    International Nuclear Information System (INIS)

    Orlov, O.O.; Kurbet, T.B.; Kalyish, O.B.; Prishchepa, O.L.

    2001-01-01

    The main ecological peculiarities of dry bor in Ukrainian Polessye were given. Research were carried out in Central Polessie of Ukraine during 1997 - 1999. Results were obtained due to spectrometric measurement of specific activity of 137 Cs in fruit bodies of mushrooms and in the soil. Species composition of macromycetes was divided on homogeneous groups on intensity of 13 7Cs accumulation. Amanita porphyria, A. muscaria and Tricholoma portentosum belong to the group of weak 137 Cs accumulation from the soil (TF = 15 - 20); Amanita pantherina, A. phalloides, Cantharellus cibarius, Boletus edulis, Tricholoma flavovirens and Laccaria laccata - to the group of moderate radionuclide accumulation (TF = 30 - 55). The group of strong 137 Cs accumulation (TF = 100 - 180) consists of Suillus variegates, S. bovines and Hydnum imbricatum; and group of very strong accumulation (TF 200 - 280) - of Xerocomus badius, Lactarius rufus, Russula xerampelina, Cortinarius varius and Paxillus involutus. Cortinarius sanguineus (with TF 400) is an accumulator of 137 Cs. Comparative evaluation of dry bor was given from the point of view of purchase of edible macromycetes in it

  7. Expression of Xylella fastidiosa RpfF in citrus disrupts signaling in Xanthomonas citri subsp. citri and thereby its virulence.

    Science.gov (United States)

    Caserta, R; Picchi, S C; Takita, M A; Tomaz, J P; Pereira, W E L; Machado, M A; Ionescu, M; Lindow, S; De Souza, A A

    2014-11-01

    Xylella fastidiosa and Xanthomonas citri subsp. citri, that cause citrus variegated chlorosis (CVC) and citrus canker diseases, respectively, utilize diffusible signal factor (DSF) for quorum sensing. DSF, produced by RpfF, are similar fatty acids in both organisms, although a different set of genes is regulated by DSF in each species. Because of this similarity, Xylella fastidiosa DSF might be recognized and affect the biology of Xanthomonas citri. Therefore, transgenic Citrus sinensis and Carrizo citrange plants overexpressing the Xylella fastidiosa rpfF were inoculated with Xanthomonas citri and changes in symptoms of citrus canker were observed. X. citri biofilms formed only at wound sites on transgenic leaves and were thicker; however, bacteria were unable to break through the tissue and form pustules elsewhere. Although abundant growth of X. citri occurred at wound sites on inoculated transgenic leaves, little growth was observed on unwounded tissue. Genes in the DFS-responsive core in X. citri were downregulated in bacteria isolated from transgenic leaves. DSF-dependent expression of engA was suppressed in cells exposed to xylem sap from transgenic plants. Thus, altered symptom development appears to be due to reduced expression of virulence genes because of the presence of antagonists of DSF signaling in X. citri in rpfF-expressing plants.

  8. Identification of Xylella fastidiosa antivirulence genes: hemagglutinin adhesins contribute a biofilm maturation to X. fastidios and colonization and attenuate virulence.

    Science.gov (United States)

    Guilhabert, Magalie R; Kirkpatrick, Bruce C

    2005-08-01

    Xylella fastidosa, a gram-negative, xylem-limited bacterium, is the causal agent of several economically important plant diseases, including Pierce's disease (PD) and citrus variegated chlorosis (CVC). Until recently, the inability to transform or produce transposon mutants of X. fastidosa had been a major impediment to identifying X. fastidosa genes that mediate pathogen and plant interactions. A random transposon (Tn5) library of X. fastidosa was constructed and screened for mutants showing more severe symptoms and earlier grapevine death (hypervirulence) than did vines infected with the wild type. Seven hypervirulent mutants identified in this screen moved faster and reached higher populations than the wild type in grapevines. These results suggest that X. fastidosa attenuates its virulence in planta and that movement is important in X. fastidosa virulence. The mutated genes were sequenced and none had been described previously as antivirulence genes, although six of them showed similarity with genes of known functions in other organisms. One transposon insertion inactivated a hemagglutinin adhesin gene (PD2118), which we named HxfA. Another mutant in a second putative X. fastidosa hemagglutinin gene, PD1792 (HxfB), was constructed, and further characterization of these hxf mutants suggests that X. fastidosa hemagglutinins mediate contact between X. fastidosa cells, which results in colony formation and biofilm maturation within the xylem vessels.

  9. Analisis Strategi Keamanan Energi Cina dalam Upaya Penurunan Emisi Karbon melalui Pendekatan Konstruktivisme

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    Hidayat Chusnul Chotimah

    2017-05-01

    Full Text Available The Chinese government has finally committed itself to reducing carbon emissions after having for years refused to limit its energy consumption levels due to industrialising efforts and economic development needs. The primary reason behind the policy change is the severe, life-threatening environmental damage resulted from industrialisation processes. The Chinese government has now set out strategic measures to safeguard its energy security by harmonising its climate change and economic policies. This is done to achieve carbon emission reduction through energy conservation and green economy development by developing renewable energy such as nuclear energy, hydropower, wind power, solar power and other undiscovered alternative energy sources. The actions taken by the Chinese government are based on existing international norm, namely those stipulated by the climate change regime which in turn formulate China's identity as a responsible country in the collective efforts to reduce carbon emissions. Operating on the basis of climate change norms, China interacts with other actors within the international structure to gather variegated knowledge on the issues at hand. With this accumulated knowledge as guidance, China enacts strategic-instrumental and resources-instrumental deliberations in its commitment to reduce carbon emissions.

  10. Chemical characterization of Citrus sinensis grafted on C. limonia and the effect of some isolated compounds on the growth of Xylella fastidiosa.

    Science.gov (United States)

    Ribeiro, Alan Bezerra; Abdelnur, Patrícia Verardi; Garcia, Cleverson Fernando; Belini, Adriana; Severino, Vanessa G Pasqualotto; da Silva, M Fátima das G F; Fernandes, João B; Vieira, Paulo C; de Carvalho, Sérgio A; de Souza, Alessandra A; Machado, Marcos A

    2008-09-10

    Citrus sinensis grafted on C. limonia produces a considerable number of compounds that are not common in both plants developed from germination of seeds. The chemical profile of scion and rootstock differ notably for absence in the form of flavonoids and coumarins containing C5 prenyl groups attached to the carbon atoms of aromatic and heterocyclic systems or to oxygen. Only linear pyranocoumarins xanthyletin and xanthoxyletin were found in scion. This observation indicates that the prenylated compounds once biosynthesized in the roots could have been translocated to other organs. Xylella fastidiosa colonizes the xylem of plants causing diseases on several economically important crops such as citrus variegated chlorosis (CVC). A number of flavonoids, coumarins, alkaloids, dihydrocinnamic acid derivative, anacardic acid, triterpenes, and limonoids were tested for in vitro activity on the growth of Xylella fastidiosa. Azadirachtin A was the most active. Hesperidin, which occurs in great amounts in cells of the mesophyll of the affected leaves with CVC, showed a moderate activity suggesting that it can act as a good barrier for small-size colonies from X. fastidiosa.

  11. Neoliberalization by Evaluation: Explaining the Making of Neoliberal Evaluative State

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    Diego Giannone

    2016-09-01

    Full Text Available Starting from the late seventies neoliberalism has emerged as the new global hegemonic par-adigm. Several studies demonstrated that different factors facilitated the global spread of neoliberalism, but little attention has been paid to the role played by evaluation both in legitimizing the neoliberalization of the state and in explaining the resilience of neoliberalism. The article argues that evaluation is a strategy of action which is able to emphasize the adaptive capacity of neoliberalism to different socio-political con-texts and the neoliberal purpose to depoliticize public action. The contribution aims to illustrate how eval-uation works on a twofold level. On the one hand, evaluation is a tool of global governance that acts nor-matively to homogenize states’ action consistently with some neoliberal values, such as competitiveness and economic efficiency. On the other hand, to conform to such values, variegated forms of evaluation are implemented by each state in order to introduce market rationality in non-economic domains, such as ed-ucation and health system. Referring to some empirical cases, these two overlapping processes are termed as the "evaluated state" and "evaluative state". As a result, neoliberalization by evaluation is a process in-volving the elevation of market-based principles and techniques of evaluation to the level of state-endorsed norms

  12. Search for C4 developmental mutants in Panicum maximum Jacq

    International Nuclear Information System (INIS)

    Fladung, M.

    2001-01-01

    Full text: Mutant plants are useful tools for studying developmental processes in defined genetic backgrounds by comparing them with their respective wild type forms. In this sense, developmental mutants or mutations involved in the establishment of certain leaf or flower specific traits are of special interest. In particular, the evolution of C 4 photosynthesis from C 3 precursors was accompanied by severe developmental changes in leaf morphology and anatomy. Our search of such mutants was followed by the idea to approach the evolution of the C 4 syndrome from a mutagenic point of view. Variants affecting normal development of the C 4 leaf anatomy may, in fact, represent possible regressive steps in C4 photosynthesis. Seeds of the C4 grass Panicum maximum Jacq. were mutagenized using ethylmethanesulfonate (EMS) and putative variants were isolated in the M2 generation by visual inspection. Main selection characteristics were whole plant, leaf morphology and pigmentation, and growth characteristics. The choice of a polyploid species for mutagenesis experiments was based on the need of detecting rare mutants, which are possibly lethal when using a diploid plant species. These variants could be of regulatory nature, affecting both morphology and physiology of C 4 photosynthesis early in leaf development. In total, nearly 100 variants were isolated and grown to maturity. Main isolated variants, which conforms to the prediction mentioned above, were as following: large interveinal space-1 and -3 (lis1, lis3), abnormal bundle sheath (abs), midribless (mbl) and variegated leaf -1 (var1). The variant lis1 was a short plant with leaves smaller than the wild type, and had a leaf lamina with a crinkly surface. Photosynthetically, lis1 indicates a clear regression from the C 4 to the C 3 photosynthesis type, which was correlated in the leaf lamina with an increase in the distance between small veins. The variant lis3 was not similar phenotypically to lis1, but it also had very

  13. Public sphere as assemblage: the cultural politics of roadside memorialization.

    Science.gov (United States)

    Campbell, Elaine

    2013-09-01

    This paper investigates contemporary academic accounts of the public sphere. In particular, it takes stock of post-Habermasian public sphere scholarship, and acknowledges a lively and variegated debate concerning the multiple ways in which individuals engage in contemporary political affairs. A critical eye is cast over a range of key insights which have come to establish the parameters of what 'counts' as a/the public sphere, who can be involved, and where and how communicative networks are established. This opens up the conceptual space for re-imagining a/the public sphere as an assemblage. Making use of recent developments in Deleuzian-inspired assemblage theory - most especially drawn from DeLanda's (2006) 'new philosophy of society' - the paper sets out an alternative perspective on the notion of the public sphere, and regards it as a space of connectivity brought into being through a contingent and heterogeneous assemblage of discursive, visual and performative practices. This is mapped out with reference to the cultural politics of roadside memorialization. However, a/the public sphere as an assemblage is not simply a 'social construction' brought into being through a logic of connectivity, but is an emergent and ephemeral space which reflexively nurtures and assembles the cultural politics (and political cultures) of which it is an integral part. The discussion concludes, then, with a consideration of the contribution of assemblage theory to public sphere studies. (Also see Campbell 2009a). © London School of Economics and Political Science 2013.

  14. “Bugabug ang dagat” (Rough seas: Experiencing Foucault’s heterotopia in fish trading houses

    Directory of Open Access Journals (Sweden)

    Nelson N. Turgo

    2012-06-01

    Full Text Available Places in the contemporary world are subjected to the workings of differentiating logics, foremost of which is globalization and to the other end, the counter-logic of localization, which results in, amongst others, the instantiation of differing spaces. These spaces, oftentimes co-existing and overlapping, are a result of contrapuntal forces, enacting their own colonization of places by people of varying interests. This article explores the other uses of kumisyunan (fish trading houses by magririgaton (fish vendors from a small fishing community in Quezon province that “simultaneously represent, contest, and invert” the very purpose and nature of the places’ rationale: fish trading. Heterotopia will be deployed in this article to further the ends of how a particular place could be inhabited by a number of spaces or exhibit alternate spatial possibilities and display a plethora of spatial practices within one singular location at different times in a particular spatial and temporal context. The article hopes to contribute to the further understanding of how everyday life and place is lived and reproduced in the variegated geographies of globalization in a developing economy like the Philippines.

  15. Endogenous cellulases in animals: Isolation of β-1,4-endoglucanase genes from two species of plant-parasitic cyst nematodes

    Science.gov (United States)

    Smant, Geert; Stokkermans, Jack P. W. G.; Yan, Yitang; de Boer, Jan M.; Baum, Thomas J.; Wang, Xiaohong; Hussey, Richard S.; Gommers, Fred J.; Henrissat, Bernard; Davis, Eric L.; Helder, Johannes; Schots, Arjen; Bakker, Jaap

    1998-01-01

    β-1,4-Endoglucanases (EGases, EC 3.2.1.4) degrade polysaccharides possessing β-1,4-glucan backbones such as cellulose and xyloglucan and have been found among extremely variegated taxonomic groups. Although many animal species depend on cellulose as their main energy source, most omnivores and herbivores are unable to produce EGases endogenously. So far, all previously identified EGase genes involved in the digestive system of animals originate from symbiotic microorganisms. Here we report on the synthesis of EGases in the esophageal glands of the cyst nematodes Globodera rostochiensis and Heterodera glycines. From each of the nematode species, two cDNAs were characterized and hydrophobic cluster analysis revealed that the four catalytic domains belong to family 5 of the glycosyl hydrolases (EC 3.2.1, 3.2.2, and 3.2.3). These domains show 37–44% overall amino acid identity with EGases from the bacteria Erwinia chrysanthemi, Clostridium acetobutylicum, and Bacillus subtilis. One EGase with a bacterial type of cellulose-binding domain was identified for each nematode species. The leucine-rich hydrophobic core of the signal peptide and the presence of a polyadenylated 3′ end precluded the EGases from being of bacterial origin. Cyst nematodes are obligatory plant parasites and the identified EGases presumably facilitate the intracellular migration through plant roots by partial cell wall degradation. PMID:9560201

  16. Pleomorphic Hyalinizing Angiectatic Tumor of Soft Parts

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    Hui-Chin Peng

    2010-08-01

    Full Text Available Pleomorphic hyalinizing angiectatic tumor (PHAT of soft parts is a rare, nonmetastasizing tumor of uncertain lineage which was first reported in 1996. Here, we report a case of PHAT and review the literature. A 49-year-old man presented with a soft and progressively enlarging mass over the right buttock for several years. On suspicion that the mass was a right gluteal lipoma, he underwent surgical excision. The excised lesion measured 14 × 6 × 3.5 cm. It had a variegated appearance with a white-tan to yellowish color on the cut surface. Some punctate hemorrhage and vessel thrombosis were seen. Microscopically, the tumor was a PHAT characterized by clusters of ectatic, fibrin-lined, thin-walled vessels, which were surrounded by a mitotically inert, spindled, pleomorphic, neoplastic stroma that contained a variable inflammatory component. Immunohistochemical study showed that the tumor cells were positive for CD34, and negative for S-100, HMB45 and actin. The patient experienced local recurrence 6 months later. The recurrent tumor was widely excised. No evidence of metastasis was found during the 18 months after the second operation. The recurrent lesion had a microscopic appearance that was similar to the initial lesion.

  17. Lipaemic donations: truth and consequences.

    Science.gov (United States)

    Lippi, Giuseppe; Franchini, Massimo

    2013-10-01

    The problem of using material of unsuitable quality, including "nontransparent turbid milky plasma" or more simply "turbid plasma", for producing blood components is not trivial for several epidemiological, technical, analytical, clinical and economical reasons. With some exception, most national and international guidelines mandate that blood components should preferably not be produced from lipaemic donations. The origin of lipaemic blood is variegated, and includes physiological or paraphysiological causes and metabolic disorders, whereas a broad range of common diseases and drugs can also be associated with hypertriglyceridaemia. Overall, the frequency of lipaemic donations ranges between 0.31% and 0.35%, although sporadic reports have highlighted that the frequency might be much higher, up to 13%. Lipaemic donations pose two leading problems in transfusion medicine, that are interference during laboratory testing, and safety of producing blood components from hypertriglyceridaemic materials. While the former issue can be overcome by using chemical or mechanical methods, the clinical use of lipaemic blood for producing components remains an unresolved question. Transfusion medicine should thereby embark on a landmark effort to find a universal agreement of behaviours and harmonization of policies worldwide. Copyright © 2012 Elsevier Ltd. All rights reserved.

  18. Anthropology, organ transplantation and the immune system: resituating commodity and gift exchange.

    Science.gov (United States)

    Kierans, Ciara

    2011-11-01

    This article reflects on contributions from medical anthropology to our understanding of the bio-social and bio-political implications of renal transplantation. Taking up the idea of transplantation as a 'complex', a vast assemblage of people, places, practices and procedures which intersect medical, social and cultural domains, I point to a reliance in the anthropological literature on overly pre-determined conceptual frameworks, organised around a distinct polarisation between organ giving and receiving, where one side (supply) takes analytical, and indeed moral, precedence over the other (receipt). These frameworks tend to fail us when it comes to thinking about the wider social, cultural and political implications of transplant technologies. In an attempt to offer a less polarised view, I draw attention to the material and symbolic role of the immune system in transplantation and the ways in which it simultaneously shapes opportunities for procurement and the lived realities of recipiency. This helps us see the many complex ways in which suffering and inequality are constituted all along the variegated chains of supply and demand that are internal to, and made possible by, transplantation practices themselves. Copyright © 2011 Elsevier Ltd. All rights reserved.

  19. Past, Present, and Future of Molecular and Cellular Oncology

    International Nuclear Information System (INIS)

    Galluzzi, Lorenzo; Vitale, Ilio; Kroemer, Guido

    2011-01-01

    In the last 20 years, the field of cellular and molecular oncology has been born and has moved its first steps, with an increasingly rapid pace. Hundreds of oncogenic and oncosuppressive signaling cascades have been characterized, facilitating the development of an ever more refined and variegated arsenal of diagnostic and therapeutic weapons. Furthermore, several cancer-specific features and processes have been identified that constitute promising therapeutic targets. For instance, it has been demonstrated that microRNAs can play a critical role in oncogenesis and tumor suppression. Moreover, it turned out that tumor cells frequently exhibit an extensive metabolic rewiring, can behave in a stem cell-like fashion (and hence sustain tumor growth), often constitutively activate stress response pathways that allow them to survive, can react to therapy by engaging in non-apoptotic cell death programs, and sometimes die while eliciting a tumor-specific immune response. In this Perspective article, we discuss the main issues generated by these discoveries that will be in the limelight of molecular and cellular oncology research for the next, hopefully few years.

  20. Transcriptome Analysis of the Phytobacterium Xylella fastidiosa Growing under Xylem-Based Chemical Conditions

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    Maristela Boaceff Ciraulo

    2010-01-01

    Full Text Available Xylella fastidiosa is a xylem-limited bacterium responsible for important plant diseases, like citrus-variegated chlorosis (CVC and grapevine Pierce's disease (PD. Interestingly, in vitro growth of X. fastidiosa in chemically defined media that resemble xylem fluid has been achieved, allowing studies of metabolic processes used by xylem-dwelling bacteria to thrive in such nutrient-poor conditions. Thus, we performed microarray hybridizations to compare transcriptomes of X. fastidiosa cells grown in 3G10-R, a medium that resembles grape sap, and in Periwinkle Wilt (PW, the complex medium traditionally used to cultivate X. fastidiosa. We identified 299 transcripts modulated in response to growth in these media. Some 3G10R-overexpressed genes have been shown to be upregulated in cells directly isolated from infected plants and may be involved in plant colonization, virulence and environmental competition. In contrast, cells cultivated in PW show a metabolic switch associated with increased aerobic respiration and enhanced bacterial growth rates.

  1. Citrus plastid-related gene profiling based on expressed sequence tag analyses

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    Tercilio Calsa Jr.

    2007-01-01

    Full Text Available Plastid-related sequences, derived from putative nuclear or plastome genes, were searched in a large collection of expressed sequence tags (ESTs and genomic sequences from the Citrus Biotechnology initiative in Brazil. The identified putative Citrus chloroplast gene sequences were compared to those from Arabidopsis, Eucalyptus and Pinus. Differential expression profiling for plastid-directed nuclear-encoded proteins and photosynthesis-related gene expression variation between Citrus sinensis and Citrus reticulata, when inoculated or not with Xylella fastidiosa, were also analyzed. Presumed Citrus plastome regions were more similar to Eucalyptus. Some putative genes appeared to be preferentially expressed in vegetative tissues (leaves and bark or in reproductive organs (flowers and fruits. Genes preferentially expressed in fruit and flower may be associated with hypothetical physiological functions. Expression pattern clustering analysis suggested that photosynthesis- and carbon fixation-related genes appeared to be up- or down-regulated in a resistant or susceptible Citrus species after Xylella inoculation in comparison to non-infected controls, generating novel information which may be helpful to develop novel genetic manipulation strategies to control Citrus variegated chlorosis (CVC.

  2. VapD in Xylella fastidiosa Is a Thermostable Protein with Ribonuclease Activity.

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    Juliano S Mendes

    Full Text Available Xylella fastidiosa strain 9a5c is a gram-negative phytopathogen that is the causal agent of citrus variegated chlorosis (CVC, a disease that is responsible for economic losses in Brazilian agriculture. The most well-known mechanism of pathogenicity for this bacterial pathogen is xylem vessel occlusion, which results from bacterial movement and the formation of biofilms. The molecular mechanisms underlying the virulence caused by biofilm formation are unknown. Here, we provide evidence showing that virulence-associated protein D in X. fastidiosa (Xf-VapD is a thermostable protein with ribonuclease activity. Moreover, protein expression analyses in two X. fastidiosa strains, including virulent (Xf9a5c and nonpathogenic (XfJ1a12 strains, showed that Xf-VapD was expressed during all phases of development in both strains and that increased expression was observed in Xf9a5c during biofilm growth. This study is an important step toward characterizing and improving our understanding of the biological significance of Xf-VapD and its potential functions in the CVC pathosystem.

  3. Cigarrinhas dos Citros, Vetoras da Bactéria Xylella fastidiosa Wells et al.: Pragas Potenciais para a Citricultura Sergipana

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    Ruberval Azevedo

    2015-04-01

    Abstract. The citrus industry in Brazil plays a role of great economic, social, generating jobs, income and development. Brazil is the largest producer of citrus, the State of Sergipe stands out in 5th place in national production. Among the many pest problems faced by Brazilian citrus is Citrus Variegated Chlorosis (CVC, known as the yellowing caused by the bacterium Xylella fastidiosa Wells et al. The CVC was officially identified in Brazil in 1987, in orchards of “Triângulo Mineiro” and North and northwest of the state of São Paulo. In the Northeast Region of Brazil, was found in 1996 in the municipality of Boquim Sergipe, and Bahia in 1997, the municipalities of Rio Real and Itapicuru. The aim was to review the literature on the species of leafhoppers vectors of CVC, and verify that occur in the state of Sergipe. The first symptoms are seen in the leaves, then go for the fruits and end up affecting the entire plant, and to be perceived can take between five months and two years. The main vectors of X. fastidiosa in citrus are the sharpshooters of the family Cicadellidae. In Brazil 12 sharpshooters species have already been confirmed. For the state of Sergipe, is scarce information about the Cicadellidae vectors, the data are limited to the northern coast of Bahia, except for vague quote about four genus (Oncometropia, Acrogonia, Dilobopterus and Homolodisca and three species (Homolodisca ignorata Melichar, Acrogonia sp. and Homolodisca spottii Takiya, Cavichioli & McKamey.

  4. Genomics and X-ray microanalysis indicate that Ca2+ and thiols mediate the aggregation and adhesion of Xylella fastidiosa

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    Leite B.

    2002-01-01

    Full Text Available The availability of the genome sequence of the bacterial plant pathogen Xylella fastidiosa, the causal agent of citrus variegated chlorosis, is accelerating important investigations concerning its pathogenicity. Plant vessel occlusion is critical for symptom development. The objective of the present study was to search for information that would help to explain the adhesion of X. fastidiosa cells to the xylem. Scanning electron microscopy revealed that adhesion may occur without the fastidium gum, an exopolysaccharide produced by X. fastidiosa, and X-ray microanalysis demonstrated the presence of elemental sulfur both in cells grown in vitro and in cells found inside plant vessels, indicating that the sulfur signal is generated by the pathogen surface. Calcium and magnesium peaks were detected in association with sulfur in occluded vessels. We propose an explanation for the adhesion and aggregation process. Thiol groups, maintained by the enzyme peptide methionine sulfoxide reductase, could be active on the surface of the bacteria and appear to promote cell-cell aggregation by forming disulfide bonds with thiol groups on the surface of adjacent cells. The enzyme methionine sulfoxide reductase has been shown to be an auxiliary component in the adhesiveness of some human pathogens. The negative charge conferred by the ionized thiol group could of itself constitute a mechanism of adhesion by allowing the formation of divalent cation bridges between the negatively charged bacteria and predominantly negatively charged xylem walls.

  5. Comparative analysis of differentially expressed sequence tags of sweet orange and mandarin infected with Xylella fastidiosa

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    Alessandra A. de Souza

    2007-01-01

    Full Text Available The Citrus ESTs Sequencing Project (CitEST conducted at Centro APTA Citros Sylvio Moreira/IAC has identified and catalogued ESTs representing a set of citrus genes expressed under relevant stress responses, including diseases such as citrus variegated chlorosis (CVC, caused by Xylella fastidiosa. All sweet orange (Citrus sinensis L. Osb. varieties are susceptible to X. fastidiosa. On the other hand, mandarins (C. reticulata Blanco are considered tolerant or resistant to the disease, although the bacterium can be sporadically detected within the trees, but no disease symptoms or economic losses are observed. To study their genetic responses to the presence of X. fastidiosa, we have compared EST libraries of leaf tissue of sweet orange Pêra IAC (highly susceptible cultivar to X. fastidiosa and mandarin ‘Ponkan’ (tolerant artificially infected with the bacterium. Using an in silico differential display, 172 genes were found to be significantly differentially expressed in such conditions. Sweet orange presented an increase in expression of photosynthesis related genes that could reveal a strategy to counterbalance a possible lower photosynthetic activity resulting from early effects of the bacterial colonization in affected plants. On the other hand, mandarin showed an active multi-component defense response against the bacterium similar to the non-host resistance pattern.

  6. The complex biogeography of the plant pathogen Xylella fastidiosa: genetic evidence of introductions and Subspecific introgression in Central America.

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    Leonard Nunney

    Full Text Available The bacterium Xylella fastidiosa is a plant pathogen with a history of economically damaging introductions of subspecies to regions where its other subspecies are native. Genetic evidence is presented demonstrating the introduction of two new taxa into Central America and their introgression into the native subspecies, X. fastidiosa subsp. fastidiosa. The data are from 10 genetic outliers detected by multilocus sequence typing (MLST of isolates from Costa Rica. Six (five from oleander, one from coffee defined a new sequence type (ST53 that carried alleles at six of the eight loci sequenced (five of the seven MLST loci diagnostic of the South American subspecies Xylella fastidiosa subsp. pauca which causes two economically damaging plant diseases, citrus variegated chlorosis and coffee leaf scorch. The two remaining loci of ST53 carried alleles from what appears to be a new South American form of X. fastidiosa. Four isolates, classified as X. fastidiosa subsp. fastidiosa, showed a low level of introgression of non-native DNA. One grapevine isolate showed introgression of an allele from X. fastidiosa subsp. pauca while the other three (from citrus and coffee showed introgression of an allele with similar ancestry to the alleles of unknown origin in ST53. The presence of X. fastidiosa subsp. pauca in Central America is troubling given its disease potential, and establishes another route for the introduction of this economically damaging subspecies into the US or elsewhere, a threat potentially compounded by the presence of a previously unknown form of X. fastidiosa.

  7. Analysis of expressed sequence tags from Citrus sinensis L. Osbeck infected with Xylella fastidiosa

    Directory of Open Access Journals (Sweden)

    Alessandra A. de Souza

    2007-01-01

    Full Text Available In order to understand the genetic responses resulting from physiological changes that occur in plants displaying citrus variegated chlorosis (CVC symptoms, we adopted a strategy of comparing two EST libraries from sweet orange [Citrus sinensis (L. Osbeck]. One of them was prepared with plants showing typical CVC symptoms caused by Xylella fastidiosa and the other with non-inoculated plants. We obtained 15,944 ESTs by sequencing the two cDNA libraries. Using an in silico hybridization strategy, 37 genes were found to have significant variation at the transcriptional level. Within this subset, 21 were up-regulated and 16 were down-regulated in plants with CVC. The main functional categories of the down-regulated transcripts in plants with CVC were associated with metabolism, protein modification, energy and transport facilitation. The majority of the up-regulated transcripts were associated with metabolism and defense response. Some transcripts associated with adaptation to stress conditions were up-regulated in plants with CVC and could explain why plants remain alive even under severe water and nutritional stress. Others of the up-regulated transcripts are related to defense response suggesting that sweet orange plants activate their defense machinery. The genes associated with stress response might be expressed as part of a secondary response related to physiological alterations caused by the infection.

  8. Aion, Eros e Hades nei frammenti caldaici

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    Silvia Lenzi

    2006-01-01

    Full Text Available The essay deals with some aspects of the knotty question concerning the presence of Aion, Eros and Hades in the variegated universe of the Chaldean Oracles, traditionally attributed to a collaboration between two “Hellenized Magi” of the second c. AD: Julian “the Chaldean” and his son Julian “the Theurgist” (even if the theurgical expertise of the elder Julian, the repository of a more ancient Chaldean tradition, might not involve a direct eastern origin but only his knowledge of “Chaldean” sciences such as astrology and divination”. Aion, who is examined here in terms of his relationship with Chronos, as a more or less autonomous divinity, is mentioned only in the context of some fragments, but his name never appears in the oracular verses. Eros (beloging also to the triad of fr. 46, along with “Faith” and “Truth” stands for an independent cosmological principle whose task is to preserve the universe and the armony of the cosmos. The Chaldean Oracles never make any explicit mention of Hades, who anyway is cited by the Byzantine scholar Psellos as an autonomous divinity of the Chaldeans.

  9. 'I expected just to walk in, get my tablets and then walk out': on framing new community pharmacy services in the English healthcare system.

    Science.gov (United States)

    Latif, Asam; Waring, Justin; Watmough, Deborah; Boyd, Matthew J; Elliott, Rachel A

    2018-04-19

    Reconfiguration of the healthcare division of labour is becoming increasingly attractive in the context of increased patient demand and resource constraints. One example is the introduction of extended roles for pharmacists to provide patients additional support to manage their medicines, while also reducing work pressures experienced by other health professionals. Understanding how such policies are framed by those delivering and receiving care has been under-theorised. Using Goffman's frame theory, we examine one newly introduced community pharmacy service (New Medicines Service (NMS)) to illustrate how a policy intended to support patient medicine-taking through the extended roles of pharmacists is framed and where this deviates from its proposed aims. Three themes emerged: (i) the spatial-material artefacts; (ii) existing discursive culture and practice around medicine-taking; and (iii) the NMS interactions that shape and govern framing and subsequent interpretation of the NMS. Our study offers an explanatory and dynamic view of the framing process with important lessons for reconfiguring medicine management policy and practice. As well as illustrating framing as being variegated, complementary or conflicting, it also shows how this plurality and fragility had consequences for patient engagement and sense-making. The consequences for engagement and recommendations for implementing future initiatives are discussed. © 2018 The Authors. Sociology of Health & Illness published by John Wiley & Sons Ltd on behalf of Foundation for SHIL.

  10. Morphological, Physiological, and Structural Responses of Two Species of Artemisia to NaCl Stress

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    Zhi-Yong Guan

    2013-01-01

    Full Text Available Effects of salt stress on Artemisia scoparia and A. vulgaris “Variegate” were examined. A. scoparia leaves became withered under NaCl treatment, whereas A. vulgaris “Variegate” leaves were not remarkably affected. Chlorophyll content decreased in both species, with a higher reduction in A. scoparia. Contents of proline, MDA, soluble carbohydrate, and Na+ increased in both species under salt stress, but A. vulgaris “Variegate” had higher level of proline and soluble carbohydrate and lower level of MDA and Na+. The ratios of K+/Na+, Ca2+/Na+, and Mg2+/Na+ in A. vulgaris “Variegate” under NaCl stress were higher. Moreover, A. vulgaris “Variegate” had higher transport selectivity of K+/Na+ from root to stem, stem to middle mature leaves, and upper newly developed leaves than A. scoparia under NaCl stress. A. vulgaris “Variegate” chloroplast maintained its morphological integrity under NaCl stress, whereas A. scoparia chloroplast lost integrity. The results indicated that A. scoparia is more sensitive to salt stress than A. vulgaris “Variegate.” Salt tolerance is mainly related to the ability of regulating osmotic pressure through the accumulation of soluble carbohydrates and proline, and the gradient distribution of K+ between roots and leaves was also contributed to osmotic pressure adjustment and improvement of plant salt tolerance.

  11. Who speaks for the climate? Considering `expert' and `authorized' claims-makers in the media (Invited)

    Science.gov (United States)

    Boykoff, M.

    2010-12-01

    In this presentation, I analyze representations of climate change in traditional and new/social media, and examine contextual elements as well as journalistic pressures that contribute to how claims-makers become ‘experts’ and/or ‘authorities’ as well as how climate-related information becomes ‘news’. These considerations seek to help make sense of how/why particular climate-related discourses find traction in traditional and new/social media, while others remain muffled or silent. In so doing, I explore how power flows through culture, politics, and society, constructing knowledge, norms, conventions and (un)truths about variegated dimensions of climate change via processes of media portrayals. I interrogate how (un)authorized voices in mass media shape claims on ‘truth’ about various facets of present day climate challenges. I argue that these significantly meld our individual and collective ‘ways of knowing’ about climate change, and in turn, vitally shape our ongoing material and social practices. The contested and complex elements explored here contribute critically to cultural interpretations via citizen perceptions and deliberations for action, as media practices stitch together formal science and policy with everyday activities in the public sphere.

  12. Orobanchaceae in the

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    Foley, Michael J.Y.

    2000-12-01

    Full Text Available Two new Orobanche taxa are described: O. austrohispanica MJ.Y. Foley, and O. crinita Viv. var. occidentalis MJ.Y. Foley. Thirty-one species or subspecies of Orobanche and one species of Cistanche have been confirmed as being present within the Flora iberica área but some others previously recorded appear to have been so erroneously. In addition, Lathraea phelypaea L. {Cistanche phelypaea (L. Cout.] and nine species of Orobanche (O. caryophyllacea Sm., O. cernua Loefl., O. elatior Sutton, O. gracilis Sm., O. ramose L., O. reticulate Wallr., O. rosmarina Beck, O. schultzii Mutel y O. variegata Wallr. are typified.Se describen una especie y una variedad nuevas de Orobanche: O. austrohispanica M J.Y. Foley y O. crinita Viv. var. occidentalis MJ.Y. Foley. Del estudio del material de herbario disponible se concluye que las Orobanchaceae están representadas en el área de Flora iberica por una especie de Cistanche y 31 de Orobanche. Se discuten las citas de varias especies de ambos géneros que han de ser consideradas erróneas o dudosas. Además se tipifican Lathraea phelypaea L. [Cistanche phelypaea (L. Cout.] y nueve especies de Orobanche (O. caryophyllacea Sm., O. cernua Loefl., O. elatior Sutton, O. gracilis Sm., O. ramose L., O. reticulate Wallr., O. rosmarina Beck, O. schultzii Mutel y O. variegate Wallr..

  13. Care Wounds: Precarious Vulnerability and the Potential of Exposure.

    Science.gov (United States)

    Cubellis, Lauren

    2018-04-07

    What does it mean to offer care when the act of caring is wounding to its giver? For peer specialists-individuals with lived experience as patients in the psychiatric system-this question shapes how they use their own histories to provide support for individuals experiencing psychiatric crisis. Peer support is unique in the way it draws on empathetic resonance and depends on carefully deployed vulnerability; where one connects with others through the recognition of shared experience and mutual hurt. For peers, care works when this guidance, reassurance, and "being with"-all of which draw upon their own stories of traumatic history and variegated suffering-mitigate the present crisis being experienced by another. Drawing on twenty-eight months of fieldwork with a peer-staffed crisis respite center in the eastern United States, I argue that the peer specialist becomes the embodiment of a novel intersection of intimacy and compensation; one that poses vulnerability not as a consequence, casualty, or risk factor in the commodification of care, but as its principle vector of resonance and the assumption on which it is based. For peers, care that works-in that it creates a mutual resonance for the recipient-becomes simultaneously care that wounds its giver.

  14. Editorial: Body Movements

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    Carina Assuncao

    2018-01-01

    Full Text Available Today, the juxtaposition between physical bodies and the gameworld is ever more fluid. Virtual Reality headsets are available at game stores with more AAA games being created for the format. The release of the Nintendo Switch and its dynamic JoyCon controllers reintroduce haptic movement based controls.  Pokémon GO’s augmented reality took gamers outdoors and has encouraged the Harry Potter franchise to follow in its mobile footsteps. Each development encourages a step further into the digital world. At the same time, the movement of bodies always has political dimensions. We live in a world where walls seem like solutions to the movement of bodies, while the mere meeting of bodies elsewhere – for sex, marriage and other reasons – is still forbidden by many states’ rules. Games and game-like interfaces have shown the ability to bend those rules, and to sometimes project other worlds and rule systems over our world in order to make bodies move and meet. For this special issue on ‘Body Movements’, Press Start invited authors to focus on embodiment, body movements, political bodies, community bodies, virtual bodies, physical bodies, feminine, masculine, trans- bodies, agency or its lack, and anything else in between. The response to this invitation was variegated, and provocative, as outlined here.

  15. Conglomerates of Stopnik

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    Jože Čar

    2003-06-01

    Full Text Available In the area between Stopnik, [ebrelje and Pick of [ebrelje the Ladinian clastic, carbonate and volcanogenic rocks, in the thickness of more then 600 m, are very diversely developed. They are situated on diferent structural blocks bounded by the middle Triassicfaults and overlay partly eroded Anisian dolomite in the footwall. Variegated volcanogenic sedimentary rocks with conglomerate lenses comprising the middle part of the Ladinian succession in the broader sorounding of Stopnik are very important for paleogeographicinterpretation of the area. Ten conglomerate lenses were separated in the middle of volcanoclastic and volcanogenic rocks on the [ebrelje structural block. Cross sections of the conglomerate lenses are some 10 m to 550 m long and 15 to 60 m thick. Their axes arearranged in the direction NW-SE. In the lower and upper part of the lithological succession in the lenses prevail conglomerates with more ordered internal structure, denser packing of pebbles and more distinct bedding but in the middle part of the succession in lensesprevail sandy conglomerates and pebbly sandstones with less ordered internal structure, less denser packing of pebbles and less distinct bedding. The pebbles consist of diferent types of limestone, dolomite, volcanic rocks, tuffs, tuffaceous sandstone and conglomerate.They represent prevailing resedimented lower Ladinian rocks. The described sedimentary rocks are interpreted as a product of alluvial fan and fan delta sedimentary complexes, which were transgressively covered by tuffaceous muddy marine sediments passing intothe Cordevolian dolomite.

  16. Past, present and future of molecular and cellular oncology

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    Lorenzo eGalluzzi

    2011-03-01

    Full Text Available In the last twenty years, the field of cellular and molecular oncology has been born and has moved its first steps, with an increasingly rapid pace. Hundreds of oncogenic and oncosuppressive signaling cascades have been characterized, facilitating the development of an ever more refined and variegated arsenal of diagnostic and therapeutic weapons. Furthermore, several cancer-specific features and processes have been identified that constitute promising therapeutic targets. For instance, it has been demonstrated that microRNAs can play a critical role in oncogenesis and tumor suppression. Moreover, it turned out that tumor cells frequently exhibit an extensive metabolic rewiring, can behave in a stem cell-like fashion (and hence sustain tumor growth, often constitutively activate stress response pathways that allow them to survive, can react to therapy by engaging in non-apoptotic cell death programs, and sometimes die while eliciting a tumor-specific immune response. In this Perspective article, we discuss the main issues generated by these discoveries that will be in the limelight of molecular and cellular oncology research for the next, hopefully few years.

  17. The DnaJ-like zinc finger domain protein PSA2 affects light acclimation and chloroplast development in Arabidopsis thaliana

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    Yan-Wen eWang

    2016-03-01

    Full Text Available The biosynthesis of chlorophylls and carotenoids and the assembly of thylakoid membranes are critical for the photoautotrophic growth of plants. Different factors are involved in these two processes. In recent years, members of the DnaJ-like zinc finger domain proteins have been found to take part in the biogenesis and/or the maintenance of plastids. One member of this family of proteins, PSA2, was recently found to localize to the thylakoid lumen and regulate the accumulation of photosystem I. In this study, we report that the silencing of PSA2 in Arabidopsis thaliana resulted in variegated leaves and retarded growth. Although both chlorophylls and total carotenoids decreased in the psa2 mutant, violaxanthin and zeaxanthin accumulated in the mutant seedlings grown under growth condition. Lower levels of non-photochemical quenching and electron transport rate were also found in the psa2 mutant seedlings under growth condition compared with those of the wild-type plants, indicating an impaired capability to acclimate to normal light irradiance when PSA2 was silenced. Moreover, we also observed an abnormal assembly of grana thylakoids and poorly developed stroma thylakoids in psa2 chloroplasts. Taken together, our results demonstrate that PSA2 is a member of the DnaJ-like zinc finger domain protein family that affects light acclimation and chloroplast development.

  18. Information structures in economics studies in the theory of markets with imperfect information

    CERN Document Server

    Nermuth, Manfred

    1982-01-01

    This book is intended as a contribution to the theory of markets with imperfect information. The subject being nearly limitless, only certain selected topics are discussed. These are outlined in the Introduction (Ch. 0). The remainder of the book is divided into three parts. All results of economic significance are contained in Parts II & III. Part I introduces the main tools for the analysis, in particular the concept of an information structure. Although most of the material presented in Part I is not original, it is hoped that the detailed and self-contained exposition will help the reader to understand not only the following pages, but also the existing technical and variegated literature on markets with imperfect information. The mathematical prerequisites needed, but not explained in the text rarely go beyond elementary calculus and probability theory. Whenever more advanced concepts are used, I have made an effort to give an intuitive explanation as well, so that the argument can also be followed o...

  19. The Open Government Data Policy as a Strategic Use of Information to Entrench Neoliberalism? The Case of Italy

    Directory of Open Access Journals (Sweden)

    Laura Franceschetti

    2016-09-01

    Full Text Available The philosophy of Open Government provides a new paradigm of innovation in public admin-istration built around three key words: transparency, participation and collaboration. Greater transparency of information about the PA and its way of working should help to regain public confidence in the institu-tions, motivating people to take a more active part in decision making processes. It should also encourage them to support the institutions by inputting their own knowledge and abilities, consequently engendering a widespread spirit of collaboration between different public authorities and between them and the pub-lic, businesses and non profit organisations, in order to relaunching the economic value of the Public Sec-tor Information (Huijboom, Van den Broek, 2011. The hypothesis behind this contribution, starting from an approach based on an interpretation of significant elements in public action (Moini 2013 and of their conceptual framework (Fischer 2003, is that through which open government, open data, social media, collective intelligence, and connectivity are key words in a new rhetoric of administrative innovation - summed up in the label ‘’government 2.0’’ - which refers to a form of public action easily seen to be drawn from the neoliberal paradigm (Jessop, 2002, even though subject to some variegated form at na-tional level

  20. Metasomatic zoning at some stratiform rare metal deposits

    International Nuclear Information System (INIS)

    Altyntsev, Yu.V.; Bazhenov, M.I.; Bepeshov, G.V.; Komarnitskij, G.M.; Petrov, I.Ya.; Serykh, A.S.

    1985-01-01

    Metasomatic zoning of stratiform deposits of rare metals (Mo, Pb, As, V, Se, U, etc.) in intermontane depresions, deposited at the postorogenic stage of Paleozoic geosyncline region development, is considered. Geochemical and geophysical characteristics of metasomatic zoning in the case of sloping and steep rock deposition are given. It is established, that in rare metal deposits in variegated deposits of molassoid formation of Middle-Upper Paleozoic the external and internal zones of metasomatic alterations are distinctly separated. The external zone is presented by mineral association: quartz + -albile + -calcite + -epidote; the internal one - by hydromica + -chlorite + -analcite, laumontite + -hematite + -ankerite + -kaolinite. Geochemical zoning is manifested quite regularly at all the deposits and it is subjected to metasomatic zoning. Changes in physical properties of rocks reflect the metasomatic zoning. The character of metasomatic alterations of rocks, geochemical zoning of metasomatites at rare metal deposits in molassoid deposits and spatially contiguous deposits in volcanogenic complexes have common features. A supposition is made on polygenic ore formation in sedimentary rocks of the depressions

  1. China’s new-age small farms and their vertical integration: agribusiness or co-ops?

    Science.gov (United States)

    Huang, Philip C C

    2011-01-01

    The future of Chinese agriculture lies not with large mechanized farms but with small capital-labor dual intensifying family farms for livestock-poultry-fish raising and vegetable-fruit cultivation. Chinese food consumption patterns have been changing from the old 8:1:1 pattern of 8 parts grain, 1 part meat, and 1 part vegetables to a 4:3:3 pattern, with a corresponding transformation in agricultural structure. Small family-farming is better suited for the new-age agriculture, including organic farming, than large-scale mechanized farming, because of the intensive, incremental, and variegated hand labor involved, not readily open to economies of scale, though compatible with economies of scope. It is also better suited to the realities of severe population pressure on land. But it requires vertical integration from cultivation to processing to marketing, albeit without horizontal integration for farming. It is against such a background that co-ops have arisen spontaneously for integrating small farms with processing and marketing. The Chinese government, however, has been supporting aggressively capitalistic agribusinesses as the preferred mode of vertical integration. At present, Chinese agriculture is poised at a crossroads, with the future organizational mode for vertical integration as yet uncertain.

  2. Technological dispute for manufacturing population

    Directory of Open Access Journals (Sweden)

    B.M. Hevko

    2017-12-01

    Full Text Available New designs of technological equipment are presented – a variegated unit for adjusting the machine for precise manufacturing of a cut or on a tool of a tester, or on a workpiece, and also a design of a control device for measuring average diameters of taps. An important element in the manufacture of cutting tools is the manufacture of appropriate technological equipment. So for the manufacture of taps, it is necessary to develop the design of technological equipment for the adjustment of the necessary design parameters, as well as the design of control devices for their parameters. To provide these operations to instruments are the requirements of accuracy, productivity, stability, cost of production and a number of others. The new design of the technological equipment for the adjustment of the machine for precise manufacturing of the cutting is presented. The design of the control device for measuring the average diameter of the taps is also given. The advantages of the device include improving the quality of processing and working out the design on the technological.

  3. Clinically Important Features of Porphyrin and Heme Metabolism and the Porphyrias

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    Siddesh Besur

    2014-11-01

    Full Text Available Heme, like chlorophyll, is a primordial molecule and is one of the fundamental pigments of life. Disorders of normal heme synthesis may cause human diseases, including certain anemias (X-linked sideroblastic anemias and porphyrias. Porphyrias are classified as hepatic and erythropoietic porphyrias based on the organ system in which heme precursors (5-aminolevulinic acid (ALA, porphobilinogen and porphyrins are chiefly overproduced. The hepatic porphyrias are further subdivided into acute porphyrias and chronic hepatic porphyrias. The acute porphyrias include acute intermittent, hereditary copro-, variegate and ALA dehydratase deficiency porphyria. Chronic hepatic porphyrias include porphyria cutanea tarda and hepatoerythropoietic porphyria. The erythropoietic porphyrias include congenital erythropoietic porphyria (Gűnther’s disease and erythropoietic protoporphyria. In this review, we summarize the key features of normal heme synthesis and its differing regulation in liver versus bone marrow. In both organs, principal regulation is exerted at the level of the first and rate-controlling enzyme, but by different molecules (heme in the liver and iron in the bone marrow. We also describe salient clinical, laboratory and genetic features of the eight types of porphyria.

  4. The Potential Health Benefits of Polyphenol-Rich Extracts from Cichorium intybus L. Studied on Caco-2 Cells Model

    Directory of Open Access Journals (Sweden)

    Elena Azzini

    2016-01-01

    Full Text Available Phytochemicals can exert their bioactivity without reaching the systemic circulation; scarcely absorbed antioxidants might reach the large bowel contributing to protection from oxidative damage-induced gastrointestinal diseases. In the present work, we aimed to study the relationship between potential activity of polyphenol-rich extracts from Cichorium intybus L. and changes in morphological characteristics on Caco-2 cells. Phytochemicals content (carotenoids and flavonoids and total antioxidant activity of Red Chicory of Treviso and Variegated Chicory of Castelfranco were evaluated. The bioactivity of polyphenol-rich extracts from chicories was studied in in vitro Caco-2 cell monolayers model. Morphological characteristics changes to test the antioxidant and/or prooxidant effect were verified by histological analysis and observed by Electronic Scansion Microscopy (SEM. On Caco-2 cell model, the polyphenols fractions from chicories have indicated a moderate antioxidant behavior until 17 μM concentration, while 70 μM and 34 μM exert cytotoxic effects for Treviso’s and Castelfranco’s Chicory, respectively, highlighted by TEER decreasing, increased permeability, and alteration of epithelium. Our findings support the beneficial effects of these products in counteracting the oxidative stress and cellular damage, induced in vitro on Caco-2 cell model, through interaction with the mucopolysaccharide complexes in the glycocalyx, maintaining in vivo a healthy and effective intestinal barrier.

  5. Chemotherapy in the management of brain metastases: the emerging role of fotemustine for patients with melanoma and NSCLC.

    Science.gov (United States)

    Addeo, Raffaele; Zappavigna, Silvia; Luce, Amalia; Facchini, Sergio; Caraglia, Michele

    2013-09-01

    An estimated 20 - 40% of cancer patients will develop brain metastases that are the most common intracranial tumors in adults. Patients with cerebral metastases represent a variegate group where selection of the most appropriate treatment depends on many patient- and disease-related factors. The impact of therapeutic option on overall survival is lacking and it is important to consider quality of life (QOL) when treating patients with brain metastases. A considerable proportion of patients are treated with palliative approaches such as whole-brain radiotherapy. The role of chemotherapy was limited in the past. Recently, several chemotherapeutic agents have been identified as potentially useful. This article examines the pharmacokinetics, efficacy and safety and tolerability of fotemustine (FTM) for the management of patients with cerebral metastasis from melanoma and non-small cell lung cancer (NSCLC). FTM is a third-generation nitrosourea that has proved its efficacy on brain metastases of melanoma and showed promising results for the treatment of brain metastasis of NSCLC because of its ability to pass the blood-brain barrier.

  6. A Simple Defined Medium for the Production of True Diketopiperazines in Xylella fastidiosa and Their Identification by Ultra-Fast Liquid Chromatography-Electrospray Ionization Ion Trap Mass Spectrometry

    Directory of Open Access Journals (Sweden)

    Michelli Massaroli da Silva

    2017-06-01

    Full Text Available Diketopiperazines can be generated by non-enzymatic cyclization of linear dipeptides at extreme temperature or pH, and the complex medium used to culture bacteria and fungi including phytone peptone and trypticase peptone, can also produce cyclic peptides by heat sterilization. As a result, it is not always clear if many diketopiperazines reported in the literature are artifacts formed by the different complex media used in microorganism growth. An ideal method for analysis of these compounds should identify whether they are either synthesized de novo from the products of primary metabolism and deliver true diketopiperazines. A simple defined medium (X. fastidiosa medium or XFM containing a single carbon source and no preformed amino acids has emerged as a method with a particularly high potential for the grown of X. fastidiosa and to produce genuine natural products. In this work, we identified a range of diketopiperazines from X. fastidiosa 9a5c growth in XFM, using Ultra-Fast Liquid Chromatography coupled with mass spectrometry. Diketopiperazines are reported for the first time from X. fastidiosa, which is responsible for citrus variegated chlorosis. We also report here fatty acids from X. fastidiosa, which were not biologically active as diffusible signals, and the role of diketopiperazines in signal transduction still remains unknown.

  7. Silencing inhibits Cre-mediated recombination of the Z/AP and Z/EG reporters in adult cells.

    Science.gov (United States)

    Long, Michael A; Rossi, Fabio M V

    2009-01-01

    The Cre-loxP system has been used to enable tissue specific activation, inactivation and mutation of many genes in vivo and has thereby greatly facilitated the genetic dissection of several cellular and developmental processes. In such studies, Cre-reporter strains, which carry a Cre-activated marker gene, are frequently utilized to validate the expression profile of Cre transgenes, to act as a surrogate marker for excision of a second allele, and to irreversibly label cells for lineage tracing experiments. We have studied three commonly used Cre-reporter strains, Z/AP, Z/EG and R26R-EYFP and have demonstrated that although each reporter can be reliably activated by Cre during early development, exposure to Cre in adult hematopoietic cells results in a much lower frequency of marker-positive cells in the Z/AP or Z/EG strains than in the R26R-EYFP strain. In marker negative cells derived from the Z/AP and Z/EG strains, the transgenic promoter is methylated and Cre-mediated recombination of the locus is inhibited. These results show that the efficiency of Cre-mediated recombination is not only dependent on the genomic context of a given loxP-flanked sequence, but also on stochastic epigenetic mechanisms underlying transgene variegation. Furthermore, our data highlights the potential shortcomings of utilizing the Z/AP and Z/EG reporters as surrogate markers of excision or in lineage tracing experiments.

  8. Characterization of Greater Middle Eastern genetic variation for enhanced disease gene discovery.

    Science.gov (United States)

    Scott, Eric M; Halees, Anason; Itan, Yuval; Spencer, Emily G; He, Yupeng; Azab, Mostafa Abdellateef; Gabriel, Stacey B; Belkadi, Aziz; Boisson, Bertrand; Abel, Laurent; Clark, Andrew G; Alkuraya, Fowzan S; Casanova, Jean-Laurent; Gleeson, Joseph G

    2016-09-01

    The Greater Middle East (GME) has been a central hub of human migration and population admixture. The tradition of consanguinity, variably practiced in the Persian Gulf region, North Africa, and Central Asia, has resulted in an elevated burden of recessive disease. Here we generated a whole-exome GME variome from 1,111 unrelated subjects. We detected substantial diversity and admixture in continental and subregional populations, corresponding to several ancient founder populations with little evidence of bottlenecks. Measured consanguinity rates were an order of magnitude above those in other sampled populations, and the GME population exhibited an increased burden of runs of homozygosity (ROHs) but showed no evidence for reduced burden of deleterious variation due to classically theorized 'genetic purging'. Applying this database to unsolved recessive conditions in the GME population reduced the number of potential disease-causing variants by four- to sevenfold. These results show variegated genetic architecture in GME populations and support future human genetic discoveries in Mendelian and population genetics.

  9. A chimeric repressor of petunia PH4 R2R3-MYB family transcription factor generates margined flowers in torenia.

    Science.gov (United States)

    Kasajima, Ichiro; Sasaki, Katsutomo

    2016-05-03

    The development of new phenotypes is key to the commercial development of the main floricultural species and cultivars. Important new phenotypes include features such as multiple-flowers, color variations, increased flower size, new petal shapes, variegation and distinctive petal margin colourations. Although their commercial use is not yet common, the transgenic technologies provide a potentially rapid means of generating interesting new phenotypes. In this report, we construct 5 vectors which we expected to change the color of the flower anthocyanins, from purple to blue, regulating vacuolar pH. When these constructs were transformed into purple torenia, we unexpectedly recovered some genotypes having slightly margined petals. These transgenic lines expressed a chimeric repressor of the petunia PhPH4 gene under the control of Cauliflower mosaic virus 35 S RNA promoter. PhPH4 is an R2R3-type MYB transcription factor. The transgenic lines lacked pigmentation in the petal margin cells both on the adaxial and abaxial surfaces. Expressions of Flavanone 3-hydroxylase (F3H), Flavonoid 3'-hydroxylase (F3'H) and Flavonoid 3'5'-hydroxylase (F3'5'H) genes were reduced in the margins of these transgenic lines, suggesting an inhibitory effect of PhPH4 repressor on anthocyanin synthesis.

  10. Fundus autofluorescence imaging of the white dot syndromes.

    Science.gov (United States)

    Yeh, Steven; Forooghian, Farzin; Wong, Wai T; Faia, Lisa J; Cukras, Catherine; Lew, Julie C; Wroblewski, Keith; Weichel, Eric D; Meyerle, Catherine B; Sen, Hatice Nida; Chew, Emily Y; Nussenblatt, Robert B

    2010-01-01

    To characterize the fundus autofluorescence (FAF) findings in patients with white dot syndromes (WDSs). Patients with WDSs underwent ophthalmic examination, fundus photography, fluorescein angiography, and FAF imaging. Patients were categorized as having no, minimal, or predominant foveal hypoautofluorescence. The severity of visual impairment was then correlated with the degree of foveal hypoautofluorescence. Fifty-five eyes of 28 patients with WDSs were evaluated. Visual acuities ranged from 20/12.5 to hand motions. Diagnoses included serpiginous choroidopathy (5 patients), birdshot retinochoroidopathy (10), multifocal choroiditis (8), relentless placoid chorioretinitis (1), presumed tuberculosis-associated serpiginouslike choroidopathy (1), acute posterior multifocal placoid pigment epitheliopathy (1), and acute zonal occult outer retinopathy (2). In active serpiginous choroidopathy, notable hyperautofluorescence in active disease distinguished it from the variegated FAF features of tuberculosis-associated serpiginouslike choroidopathy. The percentage of patients with visual acuity impairment of less than 20/40 differed among eyes with no, minimal, and predominant foveal hypoautofluorescence (P < .001). Patients with predominant foveal hypoautofluorescence demonstrated worse visual acuity than those with minimal or no foveal hypoautofluorescence (both P < .001). Fundus autofluorescence imaging is useful in the evaluation of the WDS. Visual acuity impairment is correlated with foveal hypoautofluorescence. Further studies are needed to evaluate the precise role of FAF imaging in the WDSs.

  11. Quantification of Xylella fastidiosa from Citrus Trees by Real-Time Polymerase Chain Reaction Assay.

    Science.gov (United States)

    Oliveira, Antonio C; Vallim, Marcelo A; Semighini, Camile P; Araújo, Welington L; Goldman, Gustavo H; Machado, Marcos A

    2002-10-01

    ABSTRACT Xylella fastidiosa is the causal agent of citrus variegated chlorosis (CVC), a destructive disease of sweet orange cultivars in Brazil. Polymerase chain reaction (PCR)-based assays constitute the principal diagnostic method for detection of these bacteria. In this work, we established a real-time quantitative PCR (QPCR) assay to quantify X. fastidiosa in naturally and artificially infected citrus. The X. fastidiosa cell number detected in the leaves increased according to the age of the leaf, and bacteria were not detected in the upper midrib section in young leaves, indicating temporal and spatial distribution patterns of bacteria, respectively. In addition, the X. fastidiosa cell number quantified in leaves of 'Pera' orange and 'Murcott' tangor reflected the susceptible and resistant status of these citrus cultivars. None of the 12 endophytic citrus bacteria or the four strains of X. fastidiosa nonpathogenic to citrus that were tested showed an increase in the fluorescence signal during QPCR. In contrast, all 10 CVC-causing strains exhibited an increase in fluorescence signal, thus indicating the specificity of this QPCR assay. Our QPCR provides a powerful tool for studies of different aspects of the Xylella-citrus interactions, and can be incorporated into breeding programs in order to select CVC-resistant plants more quickly.

  12. Beyond capitalism: A critique of Max Weber’s general understanding of the Islamic discourse

    Directory of Open Access Journals (Sweden)

    Joseph Jon Kaminski

    2016-07-01

    Full Text Available This article evaluates Max Weber’s overall analysis of Islam. Despite his efforts to be objective, Weber’s analysis was entrenched within a similar Orientalist discursive framework present among other Western scholars who studied non-Western traditions during the late 19th and early 20th centuries. After a review of the existing literature on Weber’s understanding of Islam and its relation to capitalism, this article critically explores other aspects of Weber’s conclusions about Islam that are equally problematic. Weber glosses over many centuries of variegated Islamic cultural history to provide a universalist account of the Islamic civilisation that reduces it to a general meta-discourse. He regularly ignores many of the more localised, regional Islamic cultural traditions altogether. As a result, Weber fails to meaningfully account for the many nuances and idiosyncrasies constitutive of the broader Islamic tradition. This article argues that Weber’s account of Islam shows a great deal of confusion and misunderstanding on his part.

  13. Double-digest RAD sequencing outperforms microsatellite loci at assigning paternity and estimating relatedness: A proof of concept in a highly promiscuous bird.

    Science.gov (United States)

    Thrasher, Derrick J; Butcher, Bronwyn G; Campagna, Leonardo; Webster, Michael S; Lovette, Irby J

    2018-02-17

    Information on genetic relationships among individuals is essential to many studies of the behaviour and ecology of wild organisms. Parentage and relatedness assays based on large numbers of single nucleotide polymorphism (SNP) loci hold substantial advantages over the microsatellite markers traditionally used for these purposes. We present a double-digest restriction site-associated DNA sequencing (ddRAD-seq) analysis pipeline that, as such, simultaneously achieves the SNP discovery and genotyping steps and which is optimized to return a statistically powerful set of SNP markers (typically 150-600 after stringent filtering) from large numbers of individuals (up to 240 per run). We explore the trade-offs inherent in this approach through a set of experiments in a species with a complex social system, the variegated fairy-wren (Malurus lamberti) and further validate it in a phylogenetically broad set of other bird species. Through direct comparisons with a parallel data set from a robust panel of highly variable microsatellite markers, we show that this ddRAD-seq approach results in substantially improved power to discriminate among potential relatives and considerably more precise estimates of relatedness coefficients. The pipeline is designed to be universally applicable to all bird species (and with minor modifications to many other taxa), to be cost- and time-efficient, and to be replicable across independent runs such that genotype data from different study periods can be combined and analysed as field samples are accumulated. © 2018 John Wiley & Sons Ltd.

  14. Characteristics of Watermelon Mosaic Virus Transmission Occurring in Korean Ginseng

    Directory of Open Access Journals (Sweden)

    Seung-Kook Choi

    2014-09-01

    Full Text Available Korean ginseng (Panax ginseng is the most popular herb for medical purpose in Korea. Recently, viral diseases from Korean ginseng showing various degrees of severe mottling, variegation and mosaic symptoms have caused quantity losses of Korean ginseng in a large number of farms. Watermelon mosaic virus (named WMVgin was identified as a causal agent for the disease of Korean ginseng. Interestingly, WMV-gin failed to infect both Korean ginseng plant and susceptible host species including cucurbitaceous plants by mechanical inoculation. However, WMV-gin could successfully infect Korean ginseng by transmission of two aphid species (Myzus persicae and Aphis gossypii. It is likely that transmission of WMV-gin was done by both the aphid species during feeding behavior of the two aphid species on Korean ginseng, though the aphids dislike feeding in Korea ginseng. Similarly, a strain of WMV (WMV-wm isolated from watermelon was transmitted successfully to Korean ginseng plant by the two aphid species, but not by mechanical inoculations. Transmission assays using M. persicae and A. gossypii clearly showed both WMV-gin and WMV-wm were not transmitted from infected Korean ginseng plant to cucurbit species that are good host species for WMV. These results suggest WMV disease occurring in Korean ginseng plant can be controlled by ecological approaches.

  15. Mottled Neuherberg (Mo sup(N)), a new male-lethal coat colour mutation of the house mouse (Mus musculus)

    International Nuclear Information System (INIS)

    Schroeder, J.H.

    1975-01-01

    A new semidominant X-chromosomal mutation, Mottled Neuherberg (Mo sup(N)), which causes coat colour variegation is described. Mo sup(N) arose in the second postirradiation generation after 2 x 200 R of X-rays (24 hours apart) to oocytes of X/O mice. Heterozygous Mo sup(N) females have irregular patches of fully and lightly coloured fur over the whole coat with curly vibrissae. Their viability is reduced, about 3% of the heterozygotes dying prenatally and 6 to 28% dying postnatally before weaning. Survivors are fertile without externally visible abnormalities. Hemizygous Mo sup(N) males die in utero after implantation. The recombination frequency between Mo sup(N) and tabby (Ta) was 3.65 +- 3.16% (with 95% -confidence limits). Therefore, it is suggested that Mo sup(N) is a new allele of the mottled (Mo) locus of the house mouse. Mo sup(N)-bearing ova seem to have a lower chance of becoming fertilized by wild-type spermatozoa than by Ta-bearing spermatozoa. (orig.) [de

  16. Long-distance signaling within Coleus x hybridus leaves; mediated by changes in intra-leaf CO2?

    Science.gov (United States)

    Stahlberg, R.; Van Volkenburgh, E.; Cleland, R. E.

    2001-01-01

    Rapid long-distance signaling in plants can occur via several mechanisms, including symplastic electric coupling and pressure waves. We show here in variegated Coleus leaves a rapid propagation of electrical signals that appears to be caused by changes in intra-leaf CO2 concentrations. Green leaf cells, when illuminated, undergo a rapid depolarization of their membrane potential (Vm) and an increase in their apoplastic pH (pHa) by a process that requires photosynthesis. This is followed by a slower hyperpolarization of Vm and apoplastic acidification, which do not require photosynthesis. White (chlorophyll-lacking) leaf cells, when in isolated white leaf segments, show only the slow response, but when in mixed (i.e. green and white) segments, the rapid Vm depolarization and increase in pHa propagate over more than 10 mm from the green to the white cells. Similarly, these responses propagate 12-20 mm from illuminated to unilluminated green cells. The fact that the propagation of these responses is eliminated when the leaf air spaces are infiltrated with solution indicates that the signal moves in the apoplast rather than the symplast. A depolarization of the mesophyll cells is induced in the dark by a decrease in apoplastic CO2 but not by an increase in pHa. These results support the hypothesis that the propagating signal for the depolarization of the white mesophyll cells is a photosynthetically induced decrease in the CO2 level of the air spaces throughout the leaf.

  17. Position of the axillary bud and mutation induction in Chrysanthemum (Dendranthema grandiflora Tzvelev) plant lets; Posicao da gema axilar e a inducao de mutacao em mudas de crisantemo (Dendranthema grandiflora Tzvelev)

    Energy Technology Data Exchange (ETDEWEB)

    Adames, Alvis Hernan; Latado, Rodrigo Rocha; Camargo, Nalza Maria; Tulmann Neto, Augusto [Centro de Energia Nuclear na Agricultura (CENA), Piracicaba, SP (Brazil). Lab. de Melhoramento de Plantas. E-mail: rrlatado@cena.usp.br

    1999-12-01

    Mutagenic treatment of multicellular meristems from vegetatively propagated plants generally results in the formation of chimeric plants. Mutated sectors can be increased and stabilized through the cutting-back method. The objective of the present research was to study the influence of application of this method in the M{sub 1}V{sub 2} population, originated from six different axillary buds from the M{sub 1}V{sub 1} chrysanthemum branches. For this purpose, rooted plants of the cultivar Ingrid (dark ping) were irradiated with 20 Gy of gamma-rays and the prune was carried out 40 days after planting. Frequency and spectrum of flower color mutants were evaluated. No mutants were observed in the control population. In the M{sub 1}V{sub 1} population, 22.1% of the total plants were mutants (white color, dark bronze, pale pink, yellow, wine, variegated and cream). Among them, 1.8% were periclinal chimeras (with only one different color from the original) and the others showed mutated sectors. No differences were observed in mutation frequency and size of mutated sector among six M{sub 1}V{sub 1} populations. The wine colored mutant was selected, multiplied and evaluated in a yield trial. This mutant named Magali was multiplied and was released as a new cultivar. (author)

  18. Social Movements and Political Agency in the Digital Age: A Communication Approach

    Directory of Open Access Journals (Sweden)

    Anastasia Kavada

    2016-08-01

    Full Text Available Digital media pose a dual challenge to conventional understandings of political agency. First, digital media destabilize long-held assumptions about the nature of collective action, about social movements and their capacity to effect change. This is because digital media are thought to facilitate more decentralized, dispersed, temporary and individualized forms of political action that subvert the notion of the collective as singular, unified, homogeneous, coherent, and mass. One way of resolving this challenge is to view the collective in looser terms, as a process rather than as a finished product, a conceptualization that can be influence our understanding not only of social movements, but also of other political actors and of society as a whole. Second, digital media highlight the need to take communication seriously in how we conceptualize both collective action and political agency. Placing communication at the centre allows us to develop this looser and more processual understanding of the collective by studying it as a process that is constituted in and through communication. Inspired by organizational communication and particularly the work of Taylor and van Every (2000, this essay proposes a conception of collective action as emerging in conversations and solidified in texts. This conceptualization allows for a more multiplex and variegated view of political agency that takes into account the specific context where agency is exercised and the power that different actors can exert in a communicative process of negotiation, persuasion and claim-making.

  19. Medical humanitarianism, human rights and political advocacy: the case of the Israeli Open Clinic.

    Science.gov (United States)

    Gottlieb, Nora; Filc, Dani; Davidovitch, Nadav

    2012-03-01

    In the context of neo-liberal retrenchments humanitarian NGOs have become alternative healthcare providers that partially fill the vacuum left by the welfare state's withdrawal from the provision of services to migrants and other marginalized populations. In many cases they thus help to build legitimacy for the state's retreat from social responsibilities. Human rights organizations play an important role in advocating for migrants' rights, but in many cases they represent a legalistic and individualized conceptualization of the right to health that limits their claims for social justice. This paper analyzes the interactions and tensions between the discourses of medical humanitarianism, human rights and political advocacy using the example of an "Open Clinic" run by an Israeli human rights organization as a case-study: In 2007 dramatically increasing patient numbers provoked an intense internal debate concerning the proposal to temporarily close the "Open Clinic" in order to press the government to take action. Based on protocols from internal meetings and parliamentary hearings and in-depth interviews, we have analyzed divergent contextualizations of the Clinic's closure. These reflect conflicting notions regarding the Clinic's variegated spectrum of roles--humanitarian, political, legitimizing, symbolic, empowering and organizational--and underlying conceptualizations of migrants' "deservingness". Our case-study thus helps to illuminate NGOs' role in the realm of migrant healthcare and points out options for a possible fruitful relationship between the divergent paradigms of medical humanitarianism, human rights and political advocacy. Copyright © 2011. Published by Elsevier Ltd.

  20. The Principles of Tax Law Equality in The Context of Direct Taxation

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    Magdalena Jarczok-Guzy

    2017-10-01

    Full Text Available Aim/purpose - The purpose of this article is a presentation of the principles of tax law equality with the aim of establishing the significance of these rules for the system of direct taxation in Poland. Only forms of taxation have been selected to conduct this analysis because they offer the most transparent and variegated picture of the real tax burden. Design/methodology/approach - The article follows the method of economic comparative analysis and offers a review of available literature on the subject. Findings - This analysis proves that the choice of different types of income taxation for natural persons conducting business activity has influence on tax burden. Research implications/limitations - The system of direct taxation in Poland has problems with equality rules because of different tax rates and tax returns which are available in Polish law. Originality/value/contribution - This article presents the problem of tax law equality in the context of economic theories developed by selected economists.

  1. Clinical practice guidelines for the diagnosis and management of melanoma: melanomas that lack classical clinical features.

    Science.gov (United States)

    Mar, Victoria J; Chamberlain, Alex J; Kelly, John W; Murray, William K; Thompson, John F

    2017-10-16

    A Cancer Council Australia multidisciplinary working group is currently revising and updating the 2008 evidence-based clinical practice guidelines for the management of cutaneous melanoma. While there have been many recent improvements in treatment options for metastatic melanoma, early diagnosis remains critical to reducing mortality from the disease. Improved awareness of the atypical presentations of this common malignancy is required to achieve this. A chapter of the new guidelines was therefore developed to aid recognition of atypical melanomas. Main recommendations: Because thick, life-threatening melanomas may lack the more classical ABCD (asymmetry, border irregularity, colour variegation, diameter > 6 mm) features of melanoma, a thorough history of the lesion with regard to change in morphology and growth over time is essential. Any lesion that is changing in morphology or growing over a period of more than one month should be excised or referred for prompt expert opinion. Changes in management as a result of the guidelines: These guidelines provide greater emphasis on improved recognition of the atypical presentations of melanoma, in particular nodular, desmoplastic and acral lentiginous subtypes, with particular awareness of hypomelanotic and amelanotic lesions.

  2. Asteroid (21) Lutetia: Disk-resolved photometric analysis of Baetica region

    Science.gov (United States)

    Hasselmann, P. H.; Barucci, M. A.; Fornasier, S.; Leyrat, C.; Carvano, J. M.; Lazzaro, D.; Sierks, H.

    2016-03-01

    (21) Lutetia has been visited by Rosetta mission on July 2010 and observed with a phase angle ranging from 0.15° to 156.8°. The Baetica region, located at the north pole has been extensively observed by OSIRIS cameras system. Baetica encompass a region called North Pole Crater Cluster (NPCC), shows a cluster of superposed craters which presents signs of variegation at the small phase angle images. For studying the location, we used 187 images distributed throughout 14 filter recorded by the NAC (Narrow Angle Camera) and WAC (Wide Angle Camera) of the OSIRIS system on-board Rosetta taken during the fly-by. Then, we photometrically modeled the region using Minnaert disk-function and Akimov phase function to obtain a resolved spectral slope map at phase angles of 5 ° and 20 ° . We observed a dichotomy between Gallicum and Danuvius-Sarnus Labes in the NPCC, but no significant phase reddening (- 0.04 ± 0.045 % μm-1deg-1). In the next step, we applied the Hapke (Hapke, B. [2008]. Icarus 195, 918-926; Hapke, B. [2012]. Theory of Reflectance and Emittance Spectroscopy, second ed. Cambridge University Press) model for the NAC F82+F22 (649.2 nm), WAC F13 (375 nm) and WAC F17 (631.6 nm) and we obtained normal albedo maps and Hapke parameter maps for NAC F82+F22. On Baetica, at 649.2 nm, the geometric albedo is 0.205 ± 0.005 , the average single-scattering albedo is 0.181 ± 0.005 , the average asymmetric factor is - 0.342 ± 0.003 , the average shadow-hiding opposition effect amplitude and width are 0.824 ± 0.002 and 0.040 ± 0.0007 , the average roughness slope is 11.45 ° ± 3 ° and the average porosity is 0.85 ± 0.002 . We are unable to confirm the presence of coherent-backscattering mechanism. In the NPCC, the normal albedo variegation among the craters walls reach 8% brighter for Gallicum Labes and 2% fainter for Danuvius Labes. The Hapke parameter maps also show a dichotomy at the opposition effect coefficients, single-scattering albedo and asymmetric factor

  3. The use of geographic information system and 1860s cadastral data to model agricultural suitability before heavy mechanization. A case study from Malta.

    Directory of Open Access Journals (Sweden)

    Gianmarco Alberti

    Full Text Available The present study seeks to understand the determinants of land agricultural suitability in Malta before heavy mechanization. A GIS-based Logistic Regression model is built on the basis of the data from mid-1800s cadastral maps (cabreo. This is the first time that such data are being used for the purpose of building a predictive model. The maps record the agricultural quality of parcels (ranging from good to lowest, which is represented by different colours. The study treats the agricultural quality as a depended variable with two levels: optimal (corresponding to the good class vs. non-optimal quality (mediocre, bad, low, and lowest classes. Seventeen predictors are isolated on the basis of literature review and data availability. Logistic Regression is used to isolate the predictors that can be considered determinants of the agricultural quality. Our model has an optimal discriminatory power (AUC: 0.92. The positive effect on land agricultural quality of the following predictors is considered and discussed: sine of the aspect (odds ratio 1.42, coast distance (2.46, Brown Rendzinas (2.31, Carbonate Raw (2.62 and Xerorendzinas (9.23 soils, distance to minor roads (4.88. Predictors resulting having a negative effect are: terrain elevation (0.96, slope (0.97, distance to the nearest geological fault lines (0.09, Terra Rossa soil (0.46, distance to secondary roads (0.19 and footpaths (0.41. The model isolates a host of topographic and cultural variables, the latter related to human mobility and landscape accessibility, which differentially contributed to the agricultural suitability, providing the bases for the creation of the fragmented and extremely variegated agricultural landscape that is the hallmark of the Maltese Islands. Our findings are also useful to suggest new questions that may be posed to the more meagre evidence from earlier periods.

  4. N-Acetylcysteine in Agriculture, a Novel Use for an Old Molecule: Focus on Controlling the Plant–Pathogen Xylella fastidiosa

    Science.gov (United States)

    Muranaka, Lígia S.; Giorgiano, Thais E.; Takita, Marco A.; Forim, Moacir R.; Silva, Luis F. C.; Coletta-Filho, Helvécio D.; Machado, Marcos A.; de Souza, Alessandra A.

    2013-01-01

    Xylella fastidiosa is a plant pathogen bacterium that causes diseases in many different crops. In citrus, it causes Citrus Variegated Chlorosis (CVC). The mechanism of pathogenicity of this bacterium is associated with its capacity to colonize and form a biofilm in the xylem vessels of host plants, and there is not yet any method to directly reduce populations of this pathogen in the field. In this study, we investigated the inhibitory effect of N-Acetylcysteine (NAC), a cysteine analogue used mainly to treat human diseases, on X. fastidiosa in different experimental conditions. Concentrations of NAC over 1 mg/mL reduced bacterial adhesion to glass surfaces, biofilm formation and the amount of exopolysaccharides (EPS). The minimal inhibitory concentration of NAC was 6 mg/mL. NAC was supplied to X. fastidiosa-infected plants in hydroponics, fertigation, and adsorbed to organic fertilizer (NAC-Fertilizer). HPLC analysis indicated that plants absorbed NAC at concentrations of 0.48 and 2.4 mg/mL but not at 6 mg/mL. Sweet orange plants with CVC symptoms treated with NAC (0.48 and 2.4 mg/mL) in hydroponics showed clear symptom remission and reduction in bacterial population, as analyzed by quantitative PCR and bacterial isolation. Experiments using fertigation and NAC-Fertilizer were done to simulate a condition closer to that normally is used in the field. For both, significant symptom remission and a reduced bacterial growth rate were observed. Using NAC-Fertilizer the lag for resurgence of symptoms on leaves after interruption of the treatment increased to around eight months. This is the first report of the anti-bacterial effect of NAC against a phytopathogenic bacterium. The results obtained in this work together with the characteristics of this molecule indicate that the use of NAC in agriculture might be a new and sustainable strategy for controlling plant pathogenic bacteria. PMID:24009716

  5. Cystic struma ovarii: a rare ovarian teratoma

    International Nuclear Information System (INIS)

    Malik, B.A.; Ali, Z.

    2011-01-01

    Struma ovarii is a unique variant of the monodermal teratomas of the ovary, which is entirely composed of thyroid tissue. It is a rare tumor which comprises 1-4% of all benign ovarian tumors. The age of presentation ranges between 6 to 74 years. It is a benign tumor and is usually unilateral. Clinical symptoms such as pelvic mass, abdominal pain and ascities occur in one third of patients, whereas rarely patients may present with pseudo-meig syndrome. Ultrasonography and computed tomography show a solid cystic mass. Histologically benign struma ovarii contain thyroid follicles of variable sizes filled with colloid. A 53 years old female presented with one month history of lower abdominal pain. The clinical and radiological findings suggested a left ovarian mass measuring 7 x 5 x 3 cm. An exploratory laparotomy was performed and the left ovarian mass was resected. The specimen was sent to AFIP for anatomical diagnosis. On gross examination, the specimen consisted of left ovary measuring 14 x 12 x 6.5 cm and weighing 527 grams. External surface of the ovary showed many multinodular areas with few cystic areas. Largest of the cyst measured 8 x 7 x 4 cm. On opening all the cysts contained yellowish watery fluid. Maximum thickness of the largest cyst wall was 0.5 cm. The solid area in the ovary measured 5 x 4 x 3 cm. On serial slicing the solid areas had whitish variegated appearance and areas of gritty hard consistency. No fallopian tube was found. Representative sections from different areas of the specimen were prepared. Histologically, the sections revealed effacement of the normal ovarian architecture by mature thyroid follicles containing colloid (Fig. 2). Some areas showed degenerated thyroid tissue with hyalinization and areas of calcification. More than 50% of the material examined contained thyroid tissue. No evidence of atypia was seen in the material examined. (author)

  6. Regulation of chloroplast biogenesis: the immutans mutant of Arabidopsis

    Energy Technology Data Exchange (ETDEWEB)

    Rodermel, Steven

    2015-11-16

    The immutans (im) variegation mutant of Arabidopsis is an ideal model to gain insight into factors that control chloroplast biogenesis. im defines the gene for PTOX, a plastoquinol terminal oxidase that participates in control of thylakoid redox. Here, we report that the im defect can be suppressed during the late stages of plant development by gigantea (gi2), which defines the gene for GIGANTEA (GI), a central component of the circadian clock that plays a poorly-understood role in diverse plant developmental processes. imgi2 mutants are late-flowering and display other well-known phenotypes associated with gi2, such as starch accumulation and resistance to oxidative stress. We show that the restoration of chloroplast biogenesis in imgi2 is caused by a developmental-specific de-repression of cytokinin signaling that involves crosstalk with signaling pathways mediated by gibberellin (GA) and SPINDLY (SPY), a GA response inhibitor. Suppression of the plastid defect in imgi2 is likely caused by a relaxation of excitation pressures in developing plastids by factors contributed by gi2, including enhanced rates of photosynthesis and increased resistance to oxidative stress. Interestingly, the suppression phenotype of imgi can be mimicked by crossing im with the starch accumulation mutant, sex1, perhaps because sex1 utilizes pathways similar to gi. We conclude that our studies provide a direct genetic linkage between GIGANTEA and chloroplast biogenesis, and we construct a model of interactions between signaling pathways mediated by gi, GA, SPY, cytokinins, and sex1 that are required for chloroplast biogenesis.

  7. Intimal cell masses in the abdominal aortas of swine fed a low-fat, low-cholesterol diet for up to twelve years of age.

    Science.gov (United States)

    Kim, D N; Schmee, J; Lee, K T; Thomas, W A

    1985-05-01

    The normal subendothelial intima of large arteries in man, swine and most other species is a variegated structure from birth onwards. In some regions it contains only a few scattered cells; in others there may be a continuous single layer of cells; and in still others the cells pile up to form what we have called intimal cell masses (ICM). The cells in the normal ICM are mostly smooth muscle cells although there is also a small resident population of monocyte-like cells. We have been studying the ICM in swine with emphasis on the abdominal aorta. We have found that atherosclerotic lesions in the abdominal aorta of swine induced by high-fat high-cholesterol diets begin by a hyperplastic reaction of the smooth muscle cells in the ICM and progress to form large lesions characterized by extensive regions of lipid-rich calcific necrotic debris similar to advanced lesions in man. Because of the putative key role of the ICM in atherogenesis we think that it is important to learn as much as possible about their natural history under conditions as normal as possible. In this report we present data on ICM in the abdominal aortas of 34 male and female Hormel miniature swine maintained on a low-fat low-cholesterol diet for up to 12 years of age. The ICM grow slowly with aging and in the distal portion of the aorta account for an average of 9% in the male and 15% in the female of the total cells in the aortic wall (intima + media).(ABSTRACT TRUNCATED AT 250 WORDS)

  8. EZH2 Impairs Human Dental Pulp Cell Mineralization via the Wnt/β-Catenin Pathway.

    Science.gov (United States)

    Li, B; Yu, F; Wu, F; Hui, T; A, P; Liao, X; Yin, B; Wang, C; Ye, L

    2018-05-01

    The enhancer of zeste homolog 2 (EZH2) is a catalytic subunit of PRC2 (polycomb repressor complex 2). It mediates gene silencing via methyltransferase activity and is involved in the determination of cell lineage. However, the function of EZH2 and the underlying mechanisms by which it affects the differentiation of human dental pulp cell (hDPC) have remained underexplored. In this research, we found that EZH2 expression decreased during the mineralization of hDPCs, with attenuated H3K27me3 (trimethylation on lysine 27 in histone H3). Overexpression of EZH2 impaired the odontogenic differentiation of hDPCs, while EZH2 without methyltransferase activity mutation (mutation of suppressed variegation of 3 to 9, enhancer of zeste and trithorax domain, EZH2ΔSET) did not display this phenotype. In addition, siRNA knockdown studies showed that EZH2 negatively modulated hDPC differentiation in vitro and inhibited mineralized nodule formation in transplanted β-tricalcium phosphate / hDPC composites. To further investigate the underlying mechanisms, we explored the Wnt/β-catenin signaling pathway in view of the fact that previous research had documented the essential role that it plays during hDPC mineralization, as well as its links to EZH2 in other cells. We demonstrated for the first time that EZH2 depletion activated the Wnt/β-catenin signaling pathway and enhanced the accumulation of β-catenin in hDPCs. Chromatin immunoprecipitation analysis suggested that these effects are attributable to the level of the EZH2-regulated H3K27me3 on the β-catenin promoter. We conclude that EZH2 plays a negative role during the odontogenic differentiation of hDPCs. Suppression of EZH2 could promote hDPC mineralization by epigenetically regulating the expression of β-catenin and activating the Wnt canonical signaling pathway.

  9. The significance of "geothermal microzonation" for the correct planning of low-grade source geothermal systems

    Science.gov (United States)

    Viccaro, Marco; Pezzino, Antonino; Belfiore, Giuseppe Maria; Campisano, Carlo

    2016-04-01

    Despite the environmental-friendly energy systems are solar thermal technologies, photovoltaic and wind power, other advantageous technologies exist, although they have not found wide development in countries such as Italy. Given the almost absent environmental impact and the rather favorable cost/benefit ratio, low-enthalpy geothermal systems are, however, likely to be of strategic importance also in Italy during the next years. The importance of geology for a sustainable exploitation of the ground through geothermal systems from low-grade sources is becoming paramount. Specifically, understanding of the lithological characteristics of the subsurface along with structures and textures of rocks is essential for a correct planning of the probe/geo-exchanger field and their associated ground source heat pumps. The complex geology of Eastern Sicily (Southern Italy), which includes volcanic, sedimentary and metamorphic units over limited extension, poses the question of how thermal conductivity of rocks is variable at the scale of restricted areas (even within the same municipality). This is the innovative concept of geothermal microzonation, i.e., how variable is the geothermal potential as a function of geology at the microscale. Some pilot areas have been therefore chosen to test how the geological features of the subsurface can influence the low-enthalpy geothermal potential of an area. Our geologically based evaluation and micro-zonation of the low-grade source geothermal potential of the selected areas have been verified to be fundamental for optimization of all the main components of a low-enthalpy geothermal system. Saving realization costs and limiting the energy consumption through correct sizing of the system are main ambitions to have sustainable development of this technology with intensive utilization of the subsurface. The variegated territory of countries such as Italy implies that these goals can be only reached if, primarily, the geological features

  10. Genetic localization and phenotypic expression of X-linked cataract (Xcat) in Mus musculus.

    Science.gov (United States)

    Favor, J; Pretsch, W

    1990-01-01

    Linkage data relative to the markers tabby and glucose-6-phosphate dehydrogenase are presented to locate X-linked cataract (Xcat) in the distal portion of the mouse X-chromosome between jimpy and hypophosphatemia. The human X-linked cataract-dental syndrome, Nance-Horan Syndrome, also maps closely to human hypophosphatemia and would suggest homology between mouse Xcat and human Nance-Horan Syndrome genes. In hemizygous males and homozygous females penetrance is complete with only slight variation in the degree of expression. Phenotypic expression in Xcat heterozygous females ranges from totally clear to totally opaque lenses. The phenotypic expression between the two lenses of a heterozygous individual could also vary between totally clear and totally opaque lenses. However, a correlation in the degree of expression between the eyes of an individual was observed. A variegated pattern of lens opacity was evident in female heterozygotes. Based on these observations, the site of gene action for the Xcat locus is suggested to be endogenous to the lens cells and the precursor cell population of the lens is concluded to be small. The identification of an X-linked cataract locus is an important contribution to the estimate of the number of mutable loci resulting in cataract, an estimate required so that dominant cataract mutagenesis results may be expressed on a per locus basis. The Xcat mutation may be a useful marker for a distal region of the mouse X-chromosome which is relatively sparsely marked and the X-linked cataract mutation may be employed in gene expression and lens development studies.

  11. Characterization of novel virulent broad-host-range phages of Xylella fastidiosa and Xanthomonas.

    Science.gov (United States)

    Ahern, Stephen J; Das, Mayukh; Bhowmick, Tushar Suvra; Young, Ry; Gonzalez, Carlos F

    2014-01-01

    The xylem-limited bacterium Xylella fastidiosa is the causal agent of several plant diseases, most notably Pierce's disease of grape and citrus variegated chlorosis. We report the isolation and characterization of the first virulent phages for X. fastidiosa, siphophages Sano and Salvo and podophages Prado and Paz, with a host range that includes Xanthomonas spp. Phages propagated on homologous hosts had observed adsorption rate constants of ~4 × 10(-12) ml cell(-1) min(-1) for X. fastidiosa strain Temecula 1 and ~5 × 10(-10) to 7 × 10(-10) ml cell(-1) min(-1) for Xanthomonas strain EC-12. Sano and Salvo exhibit >80% nucleotide identity to each other in aligned regions and are syntenic to phage BcepNazgul. We propose that phage BcepNazgul is the founding member of a novel phage type, to which Sano and Salvo belong. The lysis genes of the Nazgul-like phage type include a gene that encodes an outer membrane lipoprotein endolysin and also spanin gene families that provide insight into the evolution of the lysis pathway for phages of Gram-negative hosts. Prado and Paz, although exhibiting no significant DNA homology to each other, are new members of the phiKMV-like phage type, based on the position of the single-subunit RNA polymerase gene. The four phages are type IV pilus dependent for infection of both X. fastidiosa and Xanthomonas. The phages may be useful as agents for an effective and environmentally responsible strategy for the control of diseases caused by X. fastidiosa.

  12. N-acetylcysteine in agriculture, a novel use for an old molecule: focus on controlling the plant-pathogen Xylella fastidiosa.

    Science.gov (United States)

    Muranaka, Lígia S; Giorgiano, Thais E; Takita, Marco A; Forim, Moacir R; Silva, Luis F C; Coletta-Filho, Helvécio D; Machado, Marcos A; de Souza, Alessandra A

    2013-01-01

    Xylella fastidiosa is a plant pathogen bacterium that causes diseases in many different crops. In citrus, it causes Citrus Variegated Chlorosis (CVC). The mechanism of pathogenicity of this bacterium is associated with its capacity to colonize and form a biofilm in the xylem vessels of host plants, and there is not yet any method to directly reduce populations of this pathogen in the field. In this study, we investigated the inhibitory effect of N-Acetylcysteine (NAC), a cysteine analogue used mainly to treat human diseases, on X. fastidiosa in different experimental conditions. Concentrations of NAC over 1 mg/mL reduced bacterial adhesion to glass surfaces, biofilm formation and the amount of exopolysaccharides (EPS). The minimal inhibitory concentration of NAC was 6 mg/mL. NAC was supplied to X. fastidiosa-infected plants in hydroponics, fertigation, and adsorbed to organic fertilizer (NAC-Fertilizer). HPLC analysis indicated that plants absorbed NAC at concentrations of 0.48 and 2.4 mg/mL but not at 6 mg/mL. Sweet orange plants with CVC symptoms treated with NAC (0.48 and 2.4 mg/mL) in hydroponics showed clear symptom remission and reduction in bacterial population, as analyzed by quantitative PCR and bacterial isolation. Experiments using fertigation and NAC-Fertilizer were done to simulate a condition closer to that normally is used in the field. For both, significant symptom remission and a reduced bacterial growth rate were observed. Using NAC-Fertilizer the lag for resurgence of symptoms on leaves after interruption of the treatment increased to around eight months. This is the first report of the anti-bacterial effect of NAC against a phytopathogenic bacterium. The results obtained in this work together with the characteristics of this molecule indicate that the use of NAC in agriculture might be a new and sustainable strategy for controlling plant pathogenic bacteria.

  13. Characterization of the LysR-type transcriptional regulator YcjZ-like from Xylella fastidiosa overexpressed in Escherichia coli.

    Science.gov (United States)

    Santiago, André S; Santos, Clelton A; Mendes, Juliano S; Toledo, Marcelo A S; Beloti, Lilian L; Souza, Alessandra A; Souza, Anete P

    2015-09-01

    The Xylella fastidiosa 9a5c strain is a xylem-limited phytopathogen that is the causal agent of citrus variegated chlorosis (CVC). This bacterium is able to form a biofilm and occlude the xylem vessels of susceptible plants, which leads to significant agricultural and economic losses. Biofilms are associated with bacterial pathogenicity because they are very resistant to antibiotics and other metal-based chemicals that are used in agriculture. The X. fastidiosa YcjZ-like (XfYcjZ-like) protein belongs to the LysR-type transcriptional regulator (LTTR) family and is involved in various cellular functions that range from quorum sensing to bacterial survival. In the present study, we report the cloning, expression and purification of XfYcjZ-like, which was overexpressed in Escherichia coli. The secondary folding of the recombinant and purified protein was assessed by circular dichroism, which revealed that XfYcjZ-like contains a typical α/β fold. An initial hydrodynamic characterization showed that XfYcjZ-like is a globular tetramer in solution. In addition, using a polyclonal antibody against XfYcjZ-like, we assessed the expression profile of this protein during the different developmental phases of X. fastidiosa in in vitro cultivated biofilm cells and demonstrated that XfYcjZ-like is upregulated in planktonic cells in response to a copper shock treatment. Finally, the ability of XfYcjZ-like to interact with its own predicted promoter was confirmed in vitro, which is a typical feature of LysR. Taken together, our findings indicated that the XfYcjZ-like protein is involved in both the organization of the architecture and the maturation of the bacterial biofilm and that it is responsive to oxidative stress. Copyright © 2015 Elsevier Inc. All rights reserved.

  14. Detecting genetic introgression: high levels of intersubspecific recombination found in Xylella fastidiosa in Brazil.

    Science.gov (United States)

    Nunney, Leonard; Yuan, Xiaoli; Bromley, Robin E; Stouthamer, Richard

    2012-07-01

    Documenting the role of novel mutation versus homologous recombination in bacterial evolution, and especially in the invasion of new hosts, is central to understanding the long-term dynamics of pathogenic bacteria. We used multilocus sequence typing (MLST) to study this issue in Xylella fastidiosa subsp. pauca from Brazil, a bacterium causing citrus variegated chlorosis (CVC) and coffee leaf scorch (CLS). All 55 citrus isolates typed (plus one coffee isolate) defined three similar sequence types (STs) dominated by ST11 (85%), while the remaining 22 coffee isolates defined two STs, mainly ST16 (74%). This low level of variation masked unusually large allelic differences (>1% divergence with no intermediates) at five loci (leuA, petC, malF, cysG, and holC). We developed an introgression test to detect whether these large differences were due to introgression via homologous recombination from another X. fastidiosa subspecies. Using additional sequencing around these loci, we established that the seven randomly chosen MLST targets contained seven regions of introgression totaling 2,172 bp of 4,161 bp (52%), only 409 bp (10%) of which were detected by other recombination tests. This high level of introgression suggests the hypothesis that X. fastidiosa subsp. pauca became pathogenic on citrus and coffee (crops cultivated in Brazil for several hundred years) only recently after it gained genetic variation via intersubspecific recombination, facilitating a switch from native hosts. A candidate donor is the subspecies infecting plum in the region since 1935 (possibly X. fastidiosa subsp. multiplex). This hypothesis predicts that nonrecombinant native X. fastidiosa subsp. pauca (not yet isolated) does not cause disease in citrus or coffee.

  15. Evaluating and validating the protocol for gamma radiation induced mutations in floral distinct rosa spp

    International Nuclear Information System (INIS)

    Qadeer, M.; Hafiz, I. A.; Abbasi, N. A.; Ahmad, T.

    2015-01-01

    Among the highly fragrant Rosa species, Rosa gruss an teplitz and Rosa centifolia have high value in terms of commercial importance and economical trade. However, the absence of floral diversification and diversity in color patterns in these species acts as limiting factors in floriculture trade of these species. In the present study, we aimed to induce mutations using gamma radiations up to 120 Gy to observe the rate and the correlated effects on the several plant traits in micropropagated shoot tips of Rosa gruss an teplitz and Rosa centifolia. Irradiated shoot tips were micropropagated for one culture cycle and were acclimatized in a green house after in vitro rooting. Plants of irradiated population at 60 and 30 Gy showed 78.12 and 38.50 percentage less culture rooting percentage age as well as 23.82 and 7 percentage less flower size as compared to non irradiated population of Rosa gruss an teplitz and Rosa centifolia respectively. Moreover, flower color component a* (+ redness - green, 36.16 and 27.16) and chroma (37.77 and 27.5) depicted minimum while L* (Lightness, 45.12 and 76.64), b* (+ yellow - blue, 10.69 and -4.14) and hue angle (17.1 and -8.58) maximum value. Apart from these variations, mutants of Rosa gruss an teplitz also produced variegated, pink color and different shape flowers. Genetic variations observed among the putative mutants of Rosa gruss an teplitz and Rosa centifolia were evaluated using twelve decamer RAPD primers. Phylogenetic inferences showed large genetic diversity in putative mutants as compared to mother plant. (author)

  16. Evolution of the Lian River coastal basin in response to Quaternary marine transgressions in Southeast China

    Science.gov (United States)

    Tang, Yongjie; Zheng, Zhuo; Chen, Cong; Wang, Mengyuan; Chen, Bishan

    2018-04-01

    The coastal basin deposit in the Lian River plain is among the thickest Quaternary sequences along the southeastern coast of China. The clastic sediment accumulated in a variety of environmental settings including fluvial, channel, estuary/coastal and marine conditions. Detailed investigation of lithofacies, grain-size distributions, magnetic susceptibility, microfossils and chronology of marine core CN01, compared with regional cores, and combined with offshore seismic reflection profiles, has allowed us to correlate the spatial stratigraphy in the inner and outer plain and the seismic units. Grain size distribution analysis of core CN-01 through compositional data analysis and multivariate statistics were applied to clastic sedimentary facies and sedimentary cycles. Results show that these methods are able to derive a robust proxy information for the depositional environment of the Lian River plain. We have also been able to reconstruct deltaic evolution in response to marine transgressions. On the basis of dating results and chronostratigraphy, the estimated age of the onset of deposition in the Lian River coastal plain was more than 260 kyr BP. Three transgressive sedimentary cycles revealed in many regional cores support this age model. Detailed lithological and microfossil studies confirm that three marine (M3, M2 and M1) and three terrestrial (T3, T2 and T1) units can be distinguished. Spatial correlation between the inner plain, outer plain (typical cores characterized by marine transgression cycles) and offshore seismic reflectors reveals coherent sedimentary sequences. Two major boundaries (unconformity and erosion surfaces) can be recognized in the seismic profiles, and these correspond to weathered reddish and/or variegated clay in the study core, suggesting that Quaternary sediment changes on the Lian River plain were largely controlled by sea-level variations and coastline shift during glacial/interglacial cycles.

  17. Rheological stratification of the Hormuz Salt Formation in Iran - microstructural study of the dirty and pure rock salts from the Kuh-e-Namak (Dashti) salt diapir

    Science.gov (United States)

    Závada, Prokop; Desbois, Guillaume; Urai, Janos; Schulmann, Karel; Rahmati, Mahmoud; Lexa, Ondrej; Wollenberg, Uwe

    2014-05-01

    Significant viscosity contrasts displayed in flow structures of a mountain namakier (Kuh-e-Namak - Dashti), between 'weak' terrestrial debris bearing rock salt types and 'strong' pure rock salt types are questioned for deformation mechanisms using detailed quantitative microstructural study including crystallographic preferred orientation (CPO) mapping of halite grains. While the solid impurity rich ("dirty") rock salts contain disaggregated siltstone and dolomite interlayers, "clean" salts (debris free) reveal microscopic hematite and remnants of abundant fluid inclusions in non-recrystallized cores of porphyroclasts. Although flow in both, the recrystallized dirty and clean salt types is accommodated by combined mechanisms of pressure-solution creep (PS), grain boundary sliding (GBS) and dislocation creep accommodated grain boundary migration (GBM), their viscosity contrasts are explained by significantly slower rates of intergranular diffusion and piling up of dislocations at hematite inclusions in clean salt types. Porphyroclasts of clean salts deform by semi-brittle and plastic mechanisms with intra-crystalline damage being induced also by fluid inclusions that explode in the crystals at high fluid pressures. Boudins of clean salt types with coarse grained and original sedimentary microstructure suggest that clean rock salts are associated with dislocation creep dominated power law flow in the source layer and the diapiric stem. Rheological contrasts between both rock salt classes apply in general for the variegated and terrestrial debris rich ("dirty") Lower Hormuz and the "clean" rock salt forming the Upper Hormuz, respectively, and suggest that large strain rate gradients likely exist along horizons of mobilized salt types of different composition and microstructure.

  18. A semantic web framework to integrate cancer omics data with biological knowledge.

    Science.gov (United States)

    Holford, Matthew E; McCusker, James P; Cheung, Kei-Hoi; Krauthammer, Michael

    2012-01-25

    The RDF triple provides a simple linguistic means of describing limitless types of information. Triples can be flexibly combined into a unified data source we call a semantic model. Semantic models open new possibilities for the integration of variegated biological data. We use Semantic Web technology to explicate high throughput clinical data in the context of fundamental biological knowledge. We have extended Corvus, a data warehouse which provides a uniform interface to various forms of Omics data, by providing a SPARQL endpoint. With the querying and reasoning tools made possible by the Semantic Web, we were able to explore quantitative semantic models retrieved from Corvus in the light of systematic biological knowledge. For this paper, we merged semantic models containing genomic, transcriptomic and epigenomic data from melanoma samples with two semantic models of functional data - one containing Gene Ontology (GO) data, the other, regulatory networks constructed from transcription factor binding information. These two semantic models were created in an ad hoc manner but support a common interface for integration with the quantitative semantic models. Such combined semantic models allow us to pose significant translational medicine questions. Here, we study the interplay between a cell's molecular state and its response to anti-cancer therapy by exploring the resistance of cancer cells to Decitabine, a demethylating agent. We were able to generate a testable hypothesis to explain how Decitabine fights cancer - namely, that it targets apoptosis-related gene promoters predominantly in Decitabine-sensitive cell lines, thus conveying its cytotoxic effect by activating the apoptosis pathway. Our research provides a framework whereby similar hypotheses can be developed easily.

  19. Saturn's Irregular Moon Ymir

    Science.gov (United States)

    Denk, Tilmann; Mottola, S.

    2012-10-01

    Ymir (diameter 18 km), Saturn's second largest retrograde outer or irregular moon, has been observed six times by the Cassini narrow-angle camera (NAC) during the first 7 months in 2012. The observations span phase angles from 2° up to 102° and were taken at ranges between 15 and 18 million kilometers. From such a distance, Ymir is smaller than a pixel in the Cassini NAC. The data reveal a sidereal rotation period of 11.93 hrs, which is 1.6x longer than the previously reported value (Denk et al. 2011, EPSC/DPS #1452). Reason for this discrepancy is that the rotational light curve shows a rather uncommon 3-maxima and 3-minima shape at least in the phase angle range 50° to 100°, which was not recognizable in earlier data. The data cover several rotations from different viewing and illumination geometries and allow for a convex shape inversion with possibly a unique solution for the pole direction. The model reproduces the observed light curves to a very good accuracy without requiring albedo variegation, thereby suggesting that the lightcurve is dominated by the shape of Ymir. Among Saturn's irregular moons, the phenomenon of more than two maxima and minima at moderate to high phase angles is not unique to Ymir. At least Siarnaq and Paaliaq also show light curves with a strong deviation from a double-sine curve. Their rotation periods, however, remain unknown until more data can be taken. The light curve of Phoebe is fundamentally different to Ymir's because it is mainly shaped by local albedo differences and not by shape. Other reliable rotation periods of irregular satellites measured by Cassini include: Mundilfari 6.74 h; Kari 7.70 h; Albiorix 13.32 h; Kiviuq 21.82 h. More uncertain values are: Skathi 12 h; Bebhionn 16 h; Thrymr 27 h; Erriapus 28 h.

  20. CT and MRI diagnosis of chondrosarcoma in sinonasal and orbital region

    International Nuclear Information System (INIS)

    Yang Bentao; Wang Zhenchang; Xian Junfang; Zhang Zhengyu; Liu Zhonglin; Lan Baosen

    2006-01-01

    Objective: To investigate the CT and MRI findings of chondrosarcoma in sinonasal and orbital region so as to promote the diagnostic accuracy. Methods: All 12 cases of chondrosarcoma were verified by pathology. CT and MRI findings were analyzed retrospectively. Results: The lesions occurred in sinonasal cavity in 9 cases and in orbit in 3 cases. Pathologically 8 cases were conventional chondrosarcoma, 2 dedifferentiated chondrosarcoma, and 2 mesenchymal chondrosarcoma. On CT, chondrosarcoma revealed oval shape in 2 cases, lobular shape in 6 cases and irregular shape in 4 cases. The lesion showed stippled, ring, nodular, patchy or amorphous calcification. Postcontrast CT showed mild inhomogeneous enhancement in 3 cases. Chondrosarcoma demonstrated well-defined margin in 9 cases and hazy margin in 3 cases. Sinonasal chondrosarcoma revealed bony destruction in 7 cases. Orbital chondrosarcoma showed bony erosion invading ipsilateral frontal region in one case. On MR T 1 WI, conventional and dedifferentiated chondrosarcoma showed hypointense signal compared to brain in 6 cases and isointense signal in 4 cases. On T 2 WI, the lesions showed heterogeneous hyperintense signal in 8 cases and isointense signal in 2 cases with marked hypointense foci. Postcontrast MR imaging demonstrated mild to moderate inhomogeneous enhancement in these cases, and showed peripheral and septal enhancement in 5 cases of conventional chondrosarcoma, showing variegated appearance in 3 cases and honeycomb-like appearance in 2 cases. Mesenchymal chondrosarcoma showed isointense signal on both T 1 WI and T 2 WI, with homogeneous and heterogeneous enhancement in one case, respectively. MRI showed the extent and the associated changes of the lesions more clearly compared to CT. Conclusion: CT is the first modality of choice in the diagnosis of chondrosarcoma in sinonasal and orbital region. The typically peripheral and septal contrast enhancement can also suggest the diagnosis of chondrosarcoma on

  1. A census of mammal populations in Punta Leona Private Wildlife Refuge, Costa Rica

    Directory of Open Access Journals (Sweden)

    Joshua Timock

    2002-12-01

    Full Text Available Population sizes of six mammal species were estimated using the King method during the late dry season (March of 1996 in the Punta Leona Private Wildlife Refuge, Costa Rica. The white-faced monkey (Cebus capucinus, coati (Nasua narica and nine-banded armadillo (Dasypus novemcinctus with 148, 46, and 8 sighted individuals, respectively, demonstrated the largest populations in the refuge. The Central American spider monkey (Ateles geoffroyi, tamandua anteater (Tamandua mexicana, and variegated squirrel (Sciurus variegatoides were also included in the census. Population densities are calculated, habitat types are describes, and habitat use and activity periods are discussed.Se estimó el tamaño poblacional de seis especies de mamíferos, usando el método de King, durante la estación seca tardía (marzo de 1996 en el Refugio de Vida Silvestre de Punta Leona, Costa Rica. El mono carablanca (Cebus capucinus, el pizote (Nasua narica y el armadillo de 9 bandas (Dasypus novecinctus presentaron las poblaciones más grandes en el refugio, con 148, 46, y 8 observaciones respectivamente. El mono araña centroamericano (Ateles geoffroyi, el tamandúa hormiguero (Tamandua mexicana y la ardilla (Sciurus variegatoides también se tomaron en cuenta durante el censo. Además se calculan las densidades poblacionales, se describen los tipos de hábitat y se discute el uso de estos, así como los periodos de actividad de las especies encontradas.

  2. Phospholipases A2: enzymatic assay for snake venom (Naja naja karachiensis) with their neutralization by medicinal plants of Pakistan.

    Science.gov (United States)

    Asad, Muhammad H H B; Durr-E-Sabih; Yaqab, Tahir; Murtaza, Ghulam; Hussain, Muhammad S; Hussain, Muhammad S; Nasir, Muhammad T; Azhar, Saira; Khan, Shujaat A; Hussain, Izhar

    2014-01-01

    Phospholipases A2 (PLA2) are the most lethal and noxious component of Naja naja karachiensis venom. They are engaged to induce severe toxicities after their penetration in victims. Present study was designed to highlight hydrolytic actions of PLA. in an egg yolk mixture and to encounter their deleterious effects via medicinal plants of Pakistan. PLA2 were found to produce free fatty acids in a dose dependent manner. Venom at concentration of 0.1 mg was found to liberate 26.6 pmoles of fatty acids with a decline in pH1 of 0.2 owing to the presence of PLA2 (133 Unit/mg). When quantity of venom was increased up to 8 mg, it caused to release 133 pmoles of free fatty acids with a decrease in 1.0 pH due to abundance in PLA, (665 Unit/mg). The rest of other doses of venom (0.3-4.0 mg) was found to liberate fatty acids between these two upper and lower limits. Twenty eight medicinal plants (0.1-0.6 mg) were tried to abort PLA, hydrolytic action, however, all were found useful (50-100%) against PLA,. Bauhinia variegate L., Citrus limon (L.). Burm.f. Enicostemnma hyssopifolium (Willd.) Verdoorn, Ocimum sanctum. Psoralea corylifolia L. and Stenolobium stans (L.) D. Don were found excellent in switching off 100% phospholipases A, at their lowest concentration (0.1 mg). Three plants extract were found useful only at lower concentration (0.1 mg), however, their higher doses were seemed to aggravate venom response. Eight medicinal plants failed to neutralize PLA, rather their higher doses were found effective. Standard antidote and rest of other plants extract were able to show maximum of 50% efficiencies. Therefore, it is necessary to identify and isolate bioactive constituent(s) from above cited six medicinal plants to eradicate the problem of snake bite in the future.

  3. upSET, the Drosophila homologue of SET3, Is Required for Viability and the Proper Balance of Active and Repressive Chromatin Marks

    Directory of Open Access Journals (Sweden)

    Kyle A. McElroy

    2017-02-01

    Full Text Available Chromatin plays a critical role in faithful implementation of gene expression programs. Different post-translational modifications (PTMs of histone proteins reflect the underlying state of gene activity, and many chromatin proteins write, erase, bind, or are repelled by, these histone marks. One such protein is UpSET, the Drosophila homolog of yeast Set3 and mammalian KMT2E (MLL5. Here, we show that UpSET is necessary for the proper balance between active and repressed states. Using CRISPR/Cas-9 editing, we generated S2 cells that are mutant for upSET. We found that loss of UpSET is tolerated in S2 cells, but that heterochromatin is misregulated, as evidenced by a strong decrease in H3K9me2 levels assessed by bulk histone PTM quantification. To test whether this finding was consistent in the whole organism, we deleted the upSET coding sequence using CRISPR/Cas-9, which we found to be lethal in both sexes in flies. We were able to rescue this lethality using a tagged upSET transgene, and found that UpSET protein localizes to transcriptional start sites (TSS of active genes throughout the genome. Misregulated heterochromatin is apparent by suppressed position effect variegation of the wm4 allele in heterozygous upSET-deleted flies. Using nascent-RNA sequencing in the upSET-mutant S2 lines, we show that this result applies to heterochromatin genes generally. Our findings support a critical role for UpSET in maintaining heterochromatin, perhaps by delimiting the active chromatin environment.

  4. The Uranian satellites and Hyperion - New spectrophotometry and compositional implications

    Science.gov (United States)

    Brown, R. H.

    1983-12-01

    New reflectance spectra at 3.5 percent resolution have been obtained for Ariel, Titania, Oberon, and Hyperion in the 0.8 to 1.6-micron spectrum region. The new spectra show no absorptions other than the 1.5 micron water-ice feature (within the precision of the data), and demonstrate extension into the 0.8- to 1.6 micron region of the 1.5- to 2.5 micron spectral similarity ofo Ariel to Hyperion (Brown and Cruikshank, 1983). The new data confirm the presence of a dark, spectrally bland component on/in the water-ice surfaces of the Uranian satellites, which, with some reservations, has spectral similarities to the dark substance on the leading side of lapetus and the dark material on/in the surface of Hyperion, as well as other dark, spectrally neutral substances such as charcoal. Attempts were made to match the spectra of Ariel, Titania, and Oberon with additive reflectance mixes (aeral coverage) of fine-grained water frost and various dark components such as charcoal, lampblack, and charcoal-water-ice mixtures. The results were broad limits on the amounts of possible areal coverage of a charcoal-like spectral component on the surfaces of the Uranian satellites, but the data are not of sufficient precision to conclusively determine whether the dominant mode of contaminant dispersal is areal or voluminal. The effect of highly variegated albedos on the diameters derived by Brown, Cruikshank, and Morrison (1982) is found to be small.

  5. Provincial Poverty Dynamics in Lao PDR: A Case Study of Savannakhet

    Directory of Open Access Journals (Sweden)

    Edo Andriesse

    2012-01-01

    Full Text Available Although the average poverty level in Lao PDR has declined substantially since the beginning of economic reforms in 1986, sub-national dynamics give rise to a discussion of the trends and issues that determine and sustain provincial poverty and the variegated processes of rural transition. It appears that migration to core areas does not always generate better living standards, as migration to Vientiane Capital and Vientiane Province also results in a relocation of poverty from peripheral to core areas. This article sheds light on these problems and discusses the implications for the spatial dimensions of poverty in core provinces located on emerging Greater Mekong Subregion corridors and peripheral provinces. A case study of Savannakhet, located along the East West Economic Corridor, shows how rural households cope with the pressures arising from increasing market forces and regionalization. Based on in-depth fieldwork in the village of Ban Gnang Pho Sy, the results indicate that a shift occurred among the rural poor, in which their livelihoods changed from being based purely on subsistence agriculture to being focused increasingly upon pluriactive (commercial farming, livelihood diversification and labour migration to Thailand. Provinces located along emerging corridors experience a complex mosaic of impacts of integration due to fragmented ethnic-linguistic geographies and the varying relevance of pull versus push factors: imports versus exports, inward versus outward investments, and in- versus out-migration. In sum, rather than the neoliberal promise of a flatter socioeconomic landscape, the human geography of the Greater Mekong Subregion remains rough, due to politicization of foreign direct investments, complex land dealings and landlessness, migration patterns and rising inequality.

  6. How supportive supervision influences immunization session site practices: a quasi-experimental study in Odisha, India

    Directory of Open Access Journals (Sweden)

    Bhuputra Panda

    2015-01-01

    Full Text Available Background: Routine immunization (RI is a key child survival intervention. Ensuring acceptable standards of RI service delivery is critical for optimal outcomes. Accumulated evidences suggest that ‘supportive supervision’ improves the quality of health care services in general. During 2009–2010, the Government of Odisha and UNICEF jointly piloted this strategy in four districts to improve RI program outcomes. The present study aims to assess the effect of this strategy on improvement of skills and practices at immunization session sites. Design: A quasi-experimental ‘post-test only’ study design was adopted to compare the opinion and practices of frontline health workers and their supervisors in four intervention districts (IDs with two control districts (CDs. Altogether, we interviewed 111 supervisor–supervisee (health worker pairs using semi-structured interview schedules and case vignettes. We also directly observed health workers’ practices during immunization sessions at 111 sites. Data were analyzed with SPSS version 16.0. Results: The mean knowledge score of supervisors in CDs was significantly higher than in intervention groups. Variegated responses were obtained on case vignettes. The control group performed better in solving certain hypothetically asked problems, whereas the intervention group scored better in others. Health workers in IDs gave a lower rating to their respective supervisors’ knowledge, skill, and frequency of supervision. Logistics and vaccine availability were better in CDs. Conclusion: Notwithstanding other limitations, supportive supervision may not have independent effects on improving the quality of immunization services. Addressing systemic issues, such as the availability of essential logistics, supply chain management, timely indenting, and financial resources, could complement the supportive supervision strategy in improving immunization service delivery.

  7. STEM employment in the new economy: A labor market segmentation approach

    Science.gov (United States)

    Torres-Olave, Blanca M.

    The present study examined the extent to which the U.S. STEM labor market is stratified in terms of quality of employment. Through a series of cluster analyses and Chi-square tests on data drawn from the 2008 Survey of Income Program Participation (SIPP), the study found evidence of segmentation in the highly-skilled STEM and non-STEM samples, which included workers with a subbaccalaureate diploma or above. The cluster analyses show a pattern consistent with Labor Market Segmentation theory: Higher wages are associated with other primary employment characteristics, including health insurance and pension benefits, as well as full-time employment. In turn, lower wages showed a tendency to cluster with secondary employment characteristics, such as part-time employment, multiple employment, and restricted access to health insurance and pension benefits. The findings also suggest that women have a higher likelihood of being employed in STEM jobs with secondary characteristics. The findings reveal a far more variegated employment landscape than is usually presented in national reports of the STEM workforce. There is evidence that, while STEM employment may be more resilient than non-STEM employment to labor restructuring trends in the new economy, the former is far from immune to secondary labor characteristics. There is a need for ongoing dialogue between STEM education (at all levels), employers, policymakers, and other stakeholders to truly understand not only the barriers to equity in employment relations, but also the mechanisms that create and maintain segmentation and how they may impact women, underrepresented minorities, and the foreign-born.

  8. Procedure for measuring the solar reflectance of flat or curved roofing assemblies

    Energy Technology Data Exchange (ETDEWEB)

    Akbari, Hashem; Levinson, Ronnen [Heat Island Group, Lawrence Berkeley National Laboratory, Berkeley, CA 94720 (United States); Stern, Stephanie [Cool Roof Rating Council, Oakland, CA 94612 (United States)

    2008-07-15

    The widely used methods to measure the solar reflectance of roofing materials include ASTM standards E903 (spectrometer), C1549 (reflectometer), and E1918 (pyranometer). Standard E903 uses a spectrometer with an integrating sphere to measure the solar spectral reflectance of an area approximately 0.1 cm{sup 2}. The solar spectral reflectance is then weighted with a solar spectral irradiance to calculate the solar reflectance. Standard C1549 uses a reflectometer to measure the solar reflectance of an area approximately 5 cm{sup 2}. Both E903 and C1549 are best suited to measurement of the solar reflectance of flat, homogeneous surfaces. Standard E1918 uses a pyranometer to measure the solar reflectance of an area approximately 10 m{sup 2}, and is best applied to large surfaces that may also be rough and/or non-uniform. We describe a technique that uses a pyranometer to measure the solar reflectance of a uniform or variegated sample with diffusely reflective surface of an area of approximately 1 m{sup 2}, and use this technique (referred to as E1918A) to measure the solar reflectances of low- and high-profile tile assemblies. For 10 large (10 m{sup 2}) tile assemblies whose E1918 solar reflectances ranged from 0.10 to 0.50, the magnitude of the difference between the E1918A and E1918 measurements did not exceed 0.02 for unicolor assemblies, and did not exceed 0.03 for multicolor assemblies. (author)

  9. Directed evolution induces tributyrin hydrolysis in a virulence factor of Xylella fastidiosa using a duplicated gene as a template [v1; ref status: indexed, http://f1000r.es/48i

    Directory of Open Access Journals (Sweden)

    Hossein Gouran

    2014-09-01

    Full Text Available Duplication of genes is one of the preferred ways for natural selection to add advantageous functionality to the genome without having to reinvent the wheel with respect to catalytic efficiency and protein stability. The duplicated secretory virulence factors of Xylella fastidiosa (LesA, LesB and LesC, implicated in Pierce's disease of grape and citrus variegated chlorosis of citrus species, epitomizes the positive selection pressures exerted on advantageous genes in such pathogens. A deeper insight into the evolution of these lipases/esterases is essential to develop resistance mechanisms in transgenic plants. Directed evolution, an attempt to accelerate the evolutionary steps in the laboratory, is inherently simple when targeted for loss of function. A bigger challenge is to specify mutations that endow a new function, such as a lost functionality in a duplicated gene. Previously, we have proposed a method for enumerating candidates for mutations intended to transfer the functionality of one protein into another related protein based on the spatial and electrostatic properties of the active site residues (DECAAF. In the current work, we present in vivo validation of DECAAF by inducing tributyrin hydrolysis in LesB based on the active site similarity to LesA. The structures of these proteins have been modeled using RaptorX based on the closely related LipA protein from Xanthomonas oryzae. These mutations replicate the spatial and electrostatic conformation of LesA in the modeled structure of the mutant LesB as well, providing in silico validation before proceeding to the laborious in vivo work. Such focused mutations allows one to dissect the relevance of the duplicated genes in finer detail as compared to gene knockouts, since they do not interfere with other moonlighting functions, protein expression levels or protein-protein interaction.

  10. Sequence/structural analysis of xylem proteome emphasizes pathogenesis-related proteins, chitinases and β-1, 3-glucanases as key players in grapevine defense against Xylella fastidiosa

    Directory of Open Access Journals (Sweden)

    Sandeep Chakraborty

    2016-05-01

    Full Text Available Background. Xylella fastidiosa, the causative agent of various plant diseases including Pierce’s disease in the US, and Citrus Variegated Chlorosis in Brazil, remains a continual source of concern and economic losses, especially since almost all commercial varieties are sensitive to this Gammaproteobacteria. Differential expression of proteins in infected tissue is an established methodology to identify key elements involved in plant defense pathways. Methods. In the current work, we developed a methodology named CHURNER that emphasizes relevant protein functions from proteomic data, based on identification of proteins with similar structures that do not necessarily have sequence homology. Such clustering emphasizes protein functions which have multiple copies that are up/down-regulated, and highlights similar proteins which are differentially regulated. As a working example we present proteomic data enumerating differentially expressed proteins in xylem sap from grapevines that were infected with X. fastidiosa. Results. Analysis of this data by CHURNER highlighted pathogenesis related PR-1 proteins, reinforcing this as the foremost protein function in xylem sap involved in the grapevine defense response to X. fastidiosa. β-1, 3-glucanase, which has both anti-microbial and anti-fungal activities, is also up-regulated. Simultaneously, chitinases are found to be both up and down-regulated by CHURNER, and thus the net gain of this protein function loses its significance in the defense response. Discussion. We demonstrate how structural data can be incorporated in the pipeline of proteomic data analysis prior to making inferences on the importance of individual proteins to plant defense mechanisms. We expect CHURNER to be applicable to any proteomic data set.

  11. In vitro mutagenesis and selection for salinity tolerance and other agronomic characters in sugarcane

    International Nuclear Information System (INIS)

    Vaidya, E.R.; Patade, V.Y.; Patil, V.D.; Suprasanna, P.; Bapat, V.A.

    2006-01-01

    Full text: Sugarcane (Saccharum spp. Hybrids) is a highly polyploid plant (2n=36-170) grown in different parts of the world from the tropics to subtropics, and accounts for around 60% of the world's sugar. It is also one of the important cash crops in many developing/developed countries, with a high trade value. In this study, in vitro mutagenesis was employed in the selection of salt tolerance in popular sugarcane (Saccharum officinarum L.) cultivars CoC- 671, Co 86032 and Co 94012. Embryogenic callus cultures were subjected to gamma irradiation at different doses (10, 20, 30, 40 and 50). Radio sensitivity estimation indicated that 20 Gy irradiated cultures exhibited almost 50 per cent survival response. Relative growth rate and cell mass accumulation were recorded after irradiation and 30 days of post- irradiation culture. The plants regenerated from the calli irradiated at 20, 30 and 40 Gy were hardened and field planted to study their performance. The embryogenic callus cultures were also exposed to inhibitory levels of NaCl (42.8, 85.6, 128.3, 171.1, 213.9, 256.7 mM). The biochemical responses to the salt stress were studied in terms of proline, glycine betaine accumulation, membrane stability and cell viability. The variegated plants were also observed following gamma irradiation and were analyzed by different molecular markers (RAPD and Ac homologous transposons). The salt selected plants were further field evaluated and the desirable clones are now being evaluated in the second generation for their genetic stability. The proper evaluation of these induced variants with desirable traits may prove useful for cultivation under stress conditions

  12. The local dark matter phase-space density and impact on WIMP direct detection

    International Nuclear Information System (INIS)

    Catena, Riccardo; Ullio, Piero

    2012-01-01

    We present a new determination of the local dark matter phase-space density. This result is obtained implementing, in the limit of isotropic velocity distribution and spherical symmetry, Eddington's inversion formula, which links univocally the dark matter distribution function to the density profile, and applying, within a Bayesian framework, a Markov Chain Monte Carlo algorithm to sample mass models for the Milky Way against a broad and variegated sample of dynamical constraints. We consider three possible choices for the dark matter density profile, namely the Einasto, NFW and Burkert profiles, finding that the velocity dispersion, which characterizes the width in the distribution, tends to be larger for the Burkert case, while the escape velocity depends very weakly on the profile, with the mean value we obtain being in very good agreement with estimates from stellar kinematics. The derived dark matter phase-space densities differ significantly — most dramatically in the high velocity tails — from the model usually taken as a reference in dark matter detection studies, a Maxwell-Boltzmann distribution with velocity dispersion fixed in terms of the local circular velocity and with a sharp truncation at a given value of the escape velocity. We discuss the impact of astrophysical uncertainties on dark matter scattering rates and direct detection exclusion limits, considering a few sample cases and showing that the most sensitive ones are those for light dark matter particles and for particles scattering inelastically. As a general trend, regardless of the assumed profile, when adopting a self-consistent phase-space density, we find that rates are larger, and hence exclusion limits stronger, than with the standard Maxwell-Boltzmann approximation. Tools for applying our result on the local dark matter phase-space density to other dark matter candidates or experimental setups are provided

  13. Prospects for direct detection of dark matter in an effective theory approach

    International Nuclear Information System (INIS)

    Catena, Riccardo

    2014-01-01

    We perform the first comprehensive analysis of the prospects for direct detection of dark matter with future ton-scale detectors in the general 11-dimensional effective theory of isoscalar dark matter-nucleon interactions mediated by a heavy spin-1 or spin-0 particle. The theory includes 8 momentum and velocity dependent dark matter-nucleon interaction operators, besides the familiar spin-independent and spin-dependent operators. From a variegated sample of 27 benchmark points selected in the parameter space of the theory, we simulate independent sets of synthetic data for ton-scale Germanium and Xenon detectors. From the synthetic data, we then extract the marginal posterior probability density functions and the profile likelihoods of the model parameters. The associated Bayesian credible regions and frequentist confidence intervals allow us to assess the prospects for direct detection of dark matter at the 27 benchmark points. First, we analyze the data assuming the knowledge of the correct dark matter nucleon-interaction type, as it is commonly done for the familiar spin-independent and spin-dependent interactions. Then, we analyze the simulations extracting the dark matter-nucleon interaction type from the data directly, in contrast to standard analyses. This second approach requires an extensive exploration of the full 11-dimensional parameter space of the dark matter-nucleon effective theory. Interestingly, we identify 5 scenarios where the dark matter mass and the dark matter-nucleon interaction type can be reconstructed from the data simultaneously. We stress the importance of extracting the dark matter nucleon-interaction type from the data directly, discussing the main challenges found addressing this complex 11-dimensional problem

  14. Telomeric trans-silencing: an epigenetic repression combining RNA silencing and heterochromatin formation.

    Directory of Open Access Journals (Sweden)

    Thibaut Josse

    2007-09-01

    Full Text Available The study of P-element repression in Drosophila melanogaster led to the discovery of the telomeric Trans-Silencing Effect (TSE, a repression mechanism by which a transposon or a transgene inserted in subtelomeric heterochromatin (Telomeric Associated Sequence or TAS has the capacity to repress in trans in the female germline, a homologous transposon, or transgene located in euchromatin. TSE shows variegation among egg chambers in ovaries when silencing is incomplete. Here, we report that TSE displays an epigenetic transmission through meiosis, which involves an extrachromosomal maternally transmitted factor. We show that this silencing is highly sensitive to mutations affecting both heterochromatin formation (Su(var205 encoding Heterochromatin Protein 1 and Su(var3-7 and the repeat-associated small interfering RNA (or rasiRNA silencing pathway (aubergine, homeless, armitage, and piwi. In contrast, TSE is not sensitive to mutations affecting r2d2, which is involved in the small interfering RNA (or siRNA silencing pathway, nor is it sensitive to a mutation in loquacious, which is involved in the micro RNA (or miRNA silencing pathway. These results, taken together with the recent discovery of TAS homologous small RNAs associated to PIWI proteins, support the proposition that TSE involves a repeat-associated small interfering RNA pathway linked to heterochromatin formation, which was co-opted by the P element to establish repression of its own transposition after its recent invasion of the D. melanogaster genome. Therefore, the study of TSE provides insight into the genetic properties of a germline-specific small RNA silencing pathway.

  15. Short-Chain Fatty Acids from Periodontal Pathogens Suppress Histone Deacetylases, EZH2, and SUV39H1 To Promote Kaposi's Sarcoma-Associated Herpesvirus Replication

    Science.gov (United States)

    Yu, Xiaolan; Shahir, Abdel-Malek; Sha, Jingfeng; Feng, Zhimin; Eapen, Betty; Nithianantham, Stanley; Das, Biswajit; Karn, Jonathan; Weinberg, Aaron; Bissada, Nabil F.

    2014-01-01

    ABSTRACT Periodontal pathogens such as Porphyromonas gingivalis and Fusobacterium nucleatum produce five different short-chain fatty acids (SCFAs) as metabolic by-products. We detect significantly higher levels of SCFAs in the saliva of patients with severe periodontal disease. The different SCFAs stimulate lytic gene expression of Kaposi's sarcoma-associated herpesvirus (KSHV) dose dependently and synergistically. SCFAs inhibit class-1/2 histone deacetylases (HDACs) and downregulate expression of silent information regulator-1 (SIRT1). SCFAs also downregulate expression of enhancer of zeste homolog2 (EZH2) and suppressor of variegation 3-9 homolog1 (SUV39H1), which are two histone N-lysine methyltransferases (HLMTs). By suppressing the different components of host epigenetic regulatory machinery, SCFAs increase histone acetylation and decrease repressive histone trimethylations to transactivate the viral chromatin. These new findings provide mechanistic support that SCFAs from periodontal pathogens stimulate KSHV replication and infection in the oral cavity and are potential risk factors for development of oral Kaposi's sarcoma (KS). IMPORTANCE About 20% of KS patients develop KS lesions first in the oral cavity, while other patients never develop oral KS. It is not known if the oral microenvironment plays a role in oral KS tumor development. In this work, we demonstrate that a group of metabolic by-products, namely, short-chain fatty acids, from bacteria that cause periodontal disease promote lytic replication of KSHV, the etiological agent associated with KS. These new findings provide mechanistic support that periodontal pathogens create a unique microenvironment in the oral cavity that contributes to KSHV replication and development of oral KS. PMID:24501407

  16. The Role of piRNA-Mediated Epigenetic Silencing in the Population Dynamics of Transposable Elements in Drosophila melanogaster.

    Directory of Open Access Journals (Sweden)

    Yuh Chwen G Lee

    2015-06-01

    Full Text Available The piwi-interacting RNAs (piRNA are small RNAs that target selfish transposable elements (TEs in many animal genomes. Until now, piRNAs' role in TE population dynamics has only been discussed in the context of their suppression of TE transposition, which alone is not sufficient to account for the skewed frequency spectrum and stable containment of TEs. On the other hand, euchromatic TEs can be epigenetically silenced via piRNA-dependent heterochromatin formation and, similar to the widely known "Position-effect variegation", heterochromatin induced by TEs can "spread" into nearby genes. We hypothesized that the piRNA-mediated spread of heterochromatin from TEs into adjacent genes has deleterious functional effects and leads to selection against individual TEs. Unlike previously identified deleterious effects of TEs due to the physical disruption of DNA, the functional effect we investigated here is mediated through the epigenetic influences of TEs. We found that the repressive chromatin mark, H3K9me, is elevated in sequences adjacent to euchromatic TEs at multiple developmental stages in Drosophila melanogaster. Furthermore, the heterochromatic states of genes depend not only on the number of and distance from adjacent TEs, but also on the likelihood that their nearest TEs are targeted by piRNAs. These variations in chromatin status probably have functional consequences, causing genes near TEs to have lower expression. Importantly, we found stronger selection against TEs that lead to higher H3K9me enrichment of adjacent genes, demonstrating the pervasive evolutionary consequences of TE-induced epigenetic silencing. Because of the intrinsic biological mechanism of piRNA amplification, spread of TE heterochromatin could result in the theoretically required synergistic deleterious effects of TE insertions for stable containment of TE copy number. The indirect deleterious impact of piRNA-mediated epigenetic silencing of TEs is a previously

  17. Directed evolution induces tributyrin hydrolysis in a virulence factor of Xylella fastidiosa using a duplicated gene as a template.

    Science.gov (United States)

    Gouran, Hossein; Chakraborty, Sandeep; Rao, Basuthkar J; Asgeirsson, Bjarni; Dandekar, Abhaya

    2014-01-01

    Duplication of genes is one of the preferred ways for natural selection to add advantageous functionality to the genome without having to reinvent the wheel with respect to catalytic efficiency and protein stability. The duplicated secretory virulence factors of Xylella fastidiosa (LesA, LesB and LesC), implicated in Pierce's disease of grape and citrus variegated chlorosis of citrus species, epitomizes the positive selection pressures exerted on advantageous genes in such pathogens. A deeper insight into the evolution of these lipases/esterases is essential to develop resistance mechanisms in transgenic plants. Directed evolution, an attempt to accelerate the evolutionary steps in the laboratory, is inherently simple when targeted for loss of function. A bigger challenge is to specify mutations that endow a new function, such as a lost functionality in a duplicated gene. Previously, we have proposed a method for enumerating candidates for mutations intended to transfer the functionality of one protein into another related protein based on the spatial and electrostatic properties of the active site residues (DECAAF). In the current work, we present in vivo validation of DECAAF by inducing tributyrin hydrolysis in LesB based on the active site similarity to LesA. The structures of these proteins have been modeled using RaptorX based on the closely related LipA protein from Xanthomonas oryzae. These mutations replicate the spatial and electrostatic conformation of LesA in the modeled structure of the mutant LesB as well, providing in silico validation before proceeding to the laborious in vivo work. Such focused mutations allows one to dissect the relevance of the duplicated genes in finer detail as compared to gene knockouts, since they do not interfere with other moonlighting functions, protein expression levels or protein-protein interaction.

  18. DNA microarray-based genome comparison of a pathogenic and a nonpathogenic strain of Xylella fastidiosa delineates genes important for bacterial virulence.

    Science.gov (United States)

    Koide, Tie; Zaini, Paulo A; Moreira, Leandro M; Vêncio, Ricardo Z N; Matsukuma, Adriana Y; Durham, Alan M; Teixeira, Diva C; El-Dorry, Hamza; Monteiro, Patrícia B; da Silva, Ana Claudia R; Verjovski-Almeida, Sergio; da Silva, Aline M; Gomes, Suely L

    2004-08-01

    Xylella fastidiosa is a phytopathogenic bacterium that causes serious diseases in a wide range of economically important crops. Despite extensive comparative analyses of genome sequences of Xylella pathogenic strains from different plant hosts, nonpathogenic strains have not been studied. In this report, we show that X. fastidiosa strain J1a12, associated with citrus variegated chlorosis (CVC), is nonpathogenic when injected into citrus and tobacco plants. Furthermore, a DNA microarray-based comparison of J1a12 with 9a5c, a CVC strain that is highly pathogenic and had its genome completely sequenced, revealed that 14 coding sequences of strain 9a5c are absent or highly divergent in strain J1a12. Among them, we found an arginase and a fimbrial adhesin precursor of type III pilus, which were confirmed to be absent in the nonpathogenic strain by PCR and DNA sequencing. The absence of arginase can be correlated to the inability of J1a12 to multiply in host plants. This enzyme has been recently shown to act as a bacterial survival mechanism by down-regulating host nitric oxide production. The lack of the adhesin precursor gene is in accordance with the less aggregated phenotype observed for J1a12 cells growing in vitro. Thus, the absence of both genes can be associated with the failure of the J1a12 strain to establish and spread in citrus and tobacco plants. These results provide the first detailed comparison between a nonpathogenic strain and a pathogenic strain of X. fastidiosa, constituting an important step towards understanding the molecular basis of the disease.

  19. Mutants with increased resistance to herbicide in Guinea corn Sorghum bicolor (L.) Moench

    International Nuclear Information System (INIS)

    Odeigah, P.G.C.; Adewoyin, A.F.; Obatayo, O.O.

    1990-01-01

    Sorghum is an important staple food in many tropical countries. In Nigeria, it is extensively cultivated for food and, in recent times, as raw material for the brewing, baking and starch-making industries. We have investigated the possibilities of breeding crop cultivars of Sorghum with improved seed protein, amylase activities and resistance to herbicide by means of induced mutation. Seeds were treated by soaking them in an aqueous solution of ethyl methane sulphonate (EMS) 8 or 64 mM at room temperature 3, 6, 9, 12, 15 or 24 hours. After the treatment, the seeds were briefly rinsed in water and transferred to petri dishes containing moist filter paper for germination. The seedlings were later transplanted to loamy sand soil in plastic trays. M, seedlings were grown to maturity in the greenhouse. The M 1 contained plants with variegated leaves and other morphological abnormalities. Only the progenies of normal plants were grown for further generations. Resistance to Igran 500 E.G. (2-tert-butylamino-4-ethylamino-6-methylthio-striazine; from Ciba Geigy) was tested in M 2 seedlings by mixing 1 part per 100 (by volume) of the herbicide with the soil a day before sowing the seeds. Preliminary screening of 2,500 M 2 plants revealed a number of morphological and leaf colour mutations. 50 seedlings were more resistant to the herbicide but no seedling resistance was observed in the parent cultivar. There was a 23.43% reduction in seedling weight of the M 2 lines grown in soil treated with Igran 500, whereas the reduction in seedling weight of the original cultivar was 42.46%. The resistant M 2 seedlings had longer and better roots

  20. How supportive supervision influences immunization session site practices: a quasi-experimental study in Odisha, India.

    Science.gov (United States)

    Panda, Bhuputra; Pati, Sanghamitra; Nallala, Srinivas; Chauhan, Abhimanyu S; Anasuya, Anita; Som, Meena; Zodpey, Sanjay

    2015-01-01

    Routine immunization (RI) is a key child survival intervention. Ensuring acceptable standards of RI service delivery is critical for optimal outcomes. Accumulated evidences suggest that 'supportive supervision' improves the quality of health care services in general. During 2009-2010, the Government of Odisha and UNICEF jointly piloted this strategy in four districts to improve RI program outcomes. The present study aims to assess the effect of this strategy on improvement of skills and practices at immunization session sites. A quasi-experimental 'post-test only' study design was adopted to compare the opinion and practices of frontline health workers and their supervisors in four intervention districts (IDs) with two control districts (CDs). Altogether, we interviewed 111 supervisor-supervisee (health worker) pairs using semi-structured interview schedules and case vignettes. We also directly observed health workers' practices during immunization sessions at 111 sites. Data were analyzed with SPSS version 16.0. The mean knowledge score of supervisors in CDs was significantly higher than in intervention groups. Variegated responses were obtained on case vignettes. The control group performed better in solving certain hypothetically asked problems, whereas the intervention group scored better in others. Health workers in IDs gave a lower rating to their respective supervisors' knowledge, skill, and frequency of supervision. Logistics and vaccine availability were better in CDs. Notwithstanding other limitations, supportive supervision may not have independent effects on improving the quality of immunization services. Addressing systemic issues, such as the availability of essential logistics, supply chain management, timely indenting, and financial resources, could complement the supportive supervision strategy in improving immunization service delivery.

  1. Correlations between potassium, rubidium and cesium (133Cs and 137Cs) in sporocarps of Suillus variegatus in a Swedish boreal forest

    International Nuclear Information System (INIS)

    Vinichuk, M.; Rosen, K.; Johanson, K.J.; Dahlberg, A.

    2011-01-01

    An analysis of sporocarps of ectomycorrhizal fungi Suillus variegatus assessed whether cesium ( 133 Cs and 137 Cs) uptake was correlated with potassium (K) or rubidium (Rb) uptake. The question was whether intraspecific correlations of Rb, K and 133 Cs mass concentrations with 137 Cs activity concentrations in sporocarps were higher within, rather than among, different fungal species, and if genotypic origin of sporocarps within a population affected uptake and correlation. Sporocarps (n = 51) from a Swedish forest population affected by the fallout after the Chernobyl accident were studied. The concentrations were 31.9 ± 6.79 g kg -1 for K (mean ± SD, dwt), 0.40 ± 0.09 g kg -1 for Rb, 8.7 ± 4.36 mg kg -1 for 133 Cs and 63.7 ± 24.2 kBq kg -1 for 137 Cs. The mass concentrations of 133 Cs correlated with 137 Cs activity concentrations (r = 0.61). There was correlation between both 133 Cs concentrations (r = 0.75) and 137 Cs activity concentrations (r = 0.44) and Rb, but the 137 Cs/ 133 Cs isotopic ratio negatively correlated with Rb concentration. Concentrations of K and Rb were weakly correlated (r = 0.51). The 133 Cs mass concentrations, 137 Cs activity concentrations and 137 Cs/ 133 Cs isotopic ratios did not correlate with K concentrations. No differences between, within or, among genotypes in S. variegatus were found. This suggested the relationships between K, Rb, 133 Cs and 137 Cs in sporocarps of S. variegatus is similar to other fungal species. - Highlights: → We studied uptake of Cs ( 133 Cs and 137 Cs), K and Rb by Suillus variegates sporocarps. → Genotypic origin of fungus did not affect uptake of studied elements (isotopes). → Genotypic origin did not affect correlation between Cs ( 133 Cs and 137 Cs), K and Rb.

  2. Increased Ac excision (iae): Arabidopsis thaliana mutations affecting Ac transposition

    International Nuclear Information System (INIS)

    Jarvis, P.; Belzile, F.; Page, T.; Dean, C.

    1997-01-01

    The maize transposable element Ac is highly active in the heterologous hosts tobacco and tomato, but shows very much reduced levels of activity in Arabidopsis. A mutagenesis experiment was undertaken with the aim of identifying Arabidopsis host factors responsible for the observed low levels of Ac activity. Seed from a line carrying a single copy of the Ac element inserted into the streptomycin phosphotransferase (SPT) reporter fusion, and which displayed typically low levels of Ac activity, were mutagenized using gamma rays. Nineteen mutants displaying high levels of somatic Ac activity, as judged by their highly variegated phenotypes, were isolated after screening the M2 generation on streptomycin-containing medium. The mutations fall into two complementation groups, iae1 and iae2, are unlinked to the SPT::Ac locus and segregate in a Mendelian fashion. The iae1 mutation is recessive and the iae2 mutation is semi-dominant. The iae1 and iae2 mutants show 550- and 70-fold increases, respectively, in the average number of Ac excision sectors per cotyledon. The IAE1 locus maps to chromosome 2, whereas the SPT::Ac reporter maps to chromosome 3. A molecular study of Ac activity in the iae1 mutant confirmed the very high levels of Ac excision predicted using the phenotypic assay, but revealed only low levels of Ac re-insertion. Analyses of germinal transposition in the iae1 mutant demonstrated an average germinal excision frequency of 3% and a frequency of independent Ac re-insertions following germinal excision of 22%. The iae mutants represents a possible means of improving the efficiency of Ac/Ds transposon tagging systems in Arabidopsis, and will enable the dissection of host involvement in Ac transposition and the mechanisms employed for controlling transposable element activity

  3. Acute porphyrias in the USA: features of 108 subjects from porphyrias consortium.

    Science.gov (United States)

    Bonkovsky, Herbert L; Maddukuri, Vinaya C; Yazici, Cemal; Anderson, Karl E; Bissell, D Montgomery; Bloomer, Joseph R; Phillips, John D; Naik, Hetanshi; Peter, Inga; Baillargeon, Gwen; Bossi, Krista; Gandolfo, Laura; Light, Carrie; Bishop, David; Desnick, Robert J

    2014-12-01

    Recent descriptions of the clinical and laboratory features of subjects with acute porphyrias in the US are lacking. Our aim was to describe clinical, biochemical, and genetic features of 108 subjects. Between September 2010 and December 2012, 108 subjects with acute porphyrias (90 acute intermittent porphyrias, 9 hereditary coproporphyrias, 9 variegate porphyrias) were enrolled into an observational study. Genetic testing was performed at a central genetic testing laboratory and clinical information entered into a central database. Selected features were compared with data for adults in the US. Most subjects (88/108, 81%) were female, with self-reported onset of symptoms in the second through fourth decades of life. The most common symptom was abdominal pain. Appendectomies and cholecystectomies were common before a diagnosis of porphyria. The diagnosis was delayed by a mean of 15 years. Anxiety and depression were common, and 18% complained of chronic symptoms, especially neuropathic and other pains. The incidences of systemic arterial hypertension, chronic kidney disease, seizure disorders, and psychiatric conditions were markedly increased. Mutations of the known causative genes were found in 102/105 of those tested, with novel mutations being found in 37, including in 7/8 subjects with hereditary coproporphyria. Therapy with intravenous hematin was the most effective therapy both for treatment of acute attacks and for prevention of recurrent attacks. Acute porphyrias often remain undiagnosed for more than a decade after first symptoms develop. Intravenous hematin is the treatment of choice, both for treatment of acute attacks and for prevention of recurrent attacks. Copyright © 2014 Elsevier Inc. All rights reserved.

  4. Functional evidence for the critical amino-terminal conserved domain and key amino acids of Arabidopsis 4-HYDROXY-3-METHYLBUT-2-ENYL DIPHOSPHATE REDUCTASE.

    Science.gov (United States)

    Hsieh, Wei-Yu; Sung, Tzu-Ying; Wang, Hsin-Tzu; Hsieh, Ming-Hsiun

    2014-09-01

    The plant 4-HYDROXY-3-METHYLBUT-2-ENYL DIPHOSPHATE REDUCTASE (HDR) catalyzes the last step of the methylerythritol phosphate pathway to synthesize isopentenyl diphosphate and its allyl isomer dimethylallyl diphosphate, which are common precursors for the synthesis of plastid isoprenoids. The Arabidopsis (Arabidopsis thaliana) genomic HDR transgene-induced gene-silencing lines are albino, variegated, or pale green, confirming that HDR is essential for plants. We used Escherichia coli isoprenoid synthesis H (Protein Data Bank code 3F7T) as a template for homology modeling to identify key amino acids of Arabidopsis HDR. The predicted model reveals that cysteine (Cys)-122, Cys-213, and Cys-350 are involved in iron-sulfur cluster formation and that histidine (His)-152, His-241, glutamate (Glu)-242, Glu-243, threonine (Thr)-244, Thr-312, serine-379, and asparagine-381 are related to substrate binding or catalysis. Glu-242 and Thr-244 are conserved only in cyanobacteria, green algae, and land plants, whereas the other key amino acids are absolutely conserved from bacteria to plants. We used site-directed mutagenesis and complementation assay to confirm that these amino acids, except His-152 and His-241, were critical for Arabidopsis HDR function. Furthermore, the Arabidopsis HDR contains an extra amino-terminal domain following the transit peptide that is highly conserved from cyanobacteria, and green algae to land plants but not existing in the other bacteria. We demonstrated that the amino-terminal conserved domain was essential for Arabidopsis and cyanobacterial HDR function. Further analysis of conserved amino acids in the amino-terminal conserved domain revealed that the tyrosine-72 residue was critical for Arabidopsis HDR. These results suggest that the structure and reaction mechanism of HDR evolution have become specific for oxygen-evolving photosynthesis organisms and that HDR probably evolved independently in cyanobacteria versus other prokaryotes. © 2014

  5. Terrestrial ecosystem collapse associated to the K-Pg boundary and dinosaur extinction: palynological evidences

    Science.gov (United States)

    Bercovici, A.; Vajda, V.; Lyson, T. R.; Chester, S. G. B.; Sargis, E. J.; Pearson, D. A.; Joyce, W. G.

    2012-04-01

    We report here the discovery of the stratigraphically youngest in situ dinosaur specimen. This ceratopsian brow horn was found in southeastern Montana, in the Western Interior of the United States in a poorly rooted, silty mudstone floodplain deposit and only 13 centimeters below the palynologically defined K-Pg boundary. The boundary is identified using three criteria: 1) substantial decrease in diversity and abundance of Cretaceous pollen and spore taxa that completely disappear from the palynological record a few meters above the boundary, 2) the presence of a "fern spike", and 3) palynostratigraphical correlation to a nearby section where primary extraterrestrial impact markers are present (e.g., iridium anomaly, spherules and shocked quartz). The palynological record in the rock sequence immediately following the K-Pg boundary consistently indicates a sudden and major loss of the Cretaceous components across the North American record. During this rapid decline, the palynological assemblages are dominated by freshwater ferns (Azolla) and algae (usually Pediastrum sp. and Penetetrapites sp.) indicating generalized flooding in the area. The onset of the Paleocene sedimentation is subsequently announced by the presence of variegated beds, multiple lignite seams and small scale meandering river systems, starting with palynological associations that attest for reworking and erosion. The destabilization of terrestrial ecosystems is coincident with the markers of the K-Pg boundary, supporting a catastrophic event taking place over a very short duration. The in situ ceratopsian brow horn demonstrates that a gap devoid of non-avian dinosaur fossils in the last meters of the Cretaceous is artificial and thus inconsistent with the hypothesis that non-avian dinosaurs were extinct prior to the K-Pg boundary asteroid impact event.

  6. Aberrant methylation and associated transcriptional mobilization of Alu elements contributes to genomic instability in hypoxia.

    Science.gov (United States)

    Pal, Arnab; Srivastava, Tapasya; Sharma, Manish K; Mehndiratta, Mohit; Das, Prerna; Sinha, Subrata; Chattopadhyay, Parthaprasad

    2010-11-01

    Hypoxia is an integral part of tumorigenesis and contributes extensively to the neoplastic phenotype including drug resistance and genomic instability. It has also been reported that hypoxia results in global demethylation. Because a majority of the cytosine-phosphate-guanine (CpG) islands are found within the repeat elements of DNA, and are usually methylated under normoxic conditions, we suggested that retrotransposable Alu or short interspersed nuclear elements (SINEs) which show altered methylation and associated changes of gene expression during hypoxia, could be associated with genomic instability. U87MG glioblastoma cells were cultured in 0.1% O₂ for 6 weeks and compared with cells cultured in 21% O₂ for the same duration. Real-time PCR analysis showed a significant increase in SINE and reverse transcriptase coding long interspersed nuclear element (LINE) transcripts during hypoxia. Sequencing of bisulphite treated DNA as well as the Combined Bisulfite Restriction Analysis (COBRA) assay showed that the SINE loci studied underwent significant hypomethylation though there was patchy hypermethylation at a few sites. The inter-alu PCR profile of DNA from cells cultured under 6-week hypoxia, its 4-week revert back to normoxia and 6-week normoxia showed several changes in the band pattern indicating increased alu mediated genomic alteration. Our results show that aberrant methylation leading to increased transcription of SINE and reverse transcriptase associated LINE elements could lead to increased genomic instability in hypoxia. This might be a cause of genetic heterogeneity in tumours especially in variegated hypoxic environment and lead to a development of foci of more aggressive tumour cells. © 2009 The Authors Journal compilation © 2010 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd.

  7. Biomarkers for Detecting Mitochondrial Disorders

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    Josef Finsterer

    2018-01-01

    Full Text Available (1 Objectives: Mitochondrial disorders (MIDs are a genetically and phenotypically heterogeneous group of slowly or rapidly progressive disorders with onset from birth to senescence. Because of their variegated clinical presentation, MIDs are difficult to diagnose and are frequently missed in their early and late stages. This is why there is a need to provide biomarkers, which can be easily obtained in the case of suspecting a MID to initiate the further diagnostic work-up. (2 Methods: Literature review. (3 Results: Biomarkers for diagnostic purposes are used to confirm a suspected diagnosis and to facilitate and speed up the diagnostic work-up. For diagnosing MIDs, a number of dry and wet biomarkers have been proposed. Dry biomarkers for MIDs include the history and clinical neurological exam and structural and functional imaging studies of the brain, muscle, or myocardium by ultrasound, computed tomography (CT, magnetic resonance imaging (MRI, MR-spectroscopy (MRS, positron emission tomography (PET, or functional MRI. Wet biomarkers from blood, urine, saliva, or cerebrospinal fluid (CSF for diagnosing MIDs include lactate, creatine-kinase, pyruvate, organic acids, amino acids, carnitines, oxidative stress markers, and circulating cytokines. The role of microRNAs, cutaneous respirometry, biopsy, exercise tests, and small molecule reporters as possible biomarkers is unsolved. (4 Conclusions: The disadvantages of most putative biomarkers for MIDs are that they hardly meet the criteria for being acceptable as a biomarker (missing longitudinal studies, not validated, not easily feasible, not cheap, not ubiquitously available and that not all MIDs manifest in the brain, muscle, or myocardium. There is currently a lack of validated biomarkers for diagnosing MIDs.

  8. Getting organized: A history of amateur astronomy in the United States

    Science.gov (United States)

    Williams, Thomas R.

    2000-10-01

    During the twentieth century, American amateur astronomers attempted to form national organizations with structures and intents similar to the British Astronomical Association (BAA), an amateur organization dedicated to the advancement of astronomy and widely admired by American amateurs and professionals alike. The Society for Practical Astronomy (1910), the American Amateur Astronomers Association (1935), and the National Astronomical Association (1945) were each intended to facilitate amateur scientific contributions in BAA-like topical sections, but each of these societies failed. Founded in 1911, the American Association of Variable Star Observers (AAVSO) and the American Meteor Society (AMS) provided an alternative for amateur astronomers who were interested in those specific topics. However, it was not until 1947, when the Association of Lunar and Planetary Observers (ALPO) formed, that another large segment of amateur astronomers found a home for their interests. A second mode of national organization succeeded at mid- century and grew to include most avocational astronomers. Founded in 1947, the Astronomical League consists of regional associations of local societies, and is oriented largely towards recreational astronomy. The League sponsors annual national and regional conventions, but contributes little to scientific programs. This study concludes that avocational astronomy cannot simply be compared with professional astronomy, and instead must be viewed on its own terms as a complex and variegated field. Although the failure of American amateurs to form a BAA-like organization was at first disappointing, the specialized associations of observers, together with a separate and larger organization devoted to recreational astronomy, have served the American astronomical community well. Professional support for both types of activity was facilitated in this mode of organization. The style in which professional support is rendered appears to be important

  9. Role in diuresis of a calcitonin receptor (GPRCAL1 expressed in a distal-proximal gradient in renal organs of the mosquito Aedes aegypti (L..

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    Hyeogsun Kwon

    Full Text Available Evolution of anthropophilic hematophagy in insects resulted in the coordination of various physiological processes for survival. In female mosquitoes, a large blood meal provides proteins for egg production and as a trade-off, rapid elimination of the excess water and solutes (Na(+, Cl(- is critical for maintaining homeostasis and removing excess weight to resume flight and avoid predation. This post-prandial excretion is achieved by the concerted action of multiple hormones. Diuresis and natriuresis elicited by the calcitonin-like diuretic hormone 31 (DH(31 are believed to be mediated by a yet uncharacterized calcitonin receptor (GPRCAL in the mosquito Malpighian tubules (MTs, the renal organs. To contribute knowledge on endocrinology of mosquito diuresis we cloned GPRCAL1 from MT cDNA. This receptor is the ortholog of the DH(31 receptor from Drosophila melanogaster that is expressed in principal cells of the fruit fly MT. Immunofluorescence similarly showed AaegGPRCAL1 is present in MT principal cells in A. aegypti, however, exhibiting an overall gradient-like pattern along the tubule novel for a GPCR in insects. Variegated, cell-specific receptor expression revealed a subpopulation of otherwise phenotypically similar principal cells. To investigate the receptor contribution to fluid elimination, RNAi was followed by urine measurement assays. In vitro, MTs from females that underwent AaegGPRcal1 knock-down exhibited up to 57% decrease in the rate of fluid secretion in response to DH(31. Live females treated with AaegGPRcal1 dsRNA exhibited 30% reduction in fluid excreted after a blood meal. The RNAi-induced phenotype demonstrates the critical contribution of this single secretin-like family B GPCR to fluid excretion in invertebrates and highlights its relevance for the blood feeding adaptation. Our results with the mosquito AaegGPRCAL1 imply that the regulatory function of calcitonin-like receptors for ion and fluid transport in renal organs

  10. Global gene expression under nitrogen starvation in Xylella fastidiosa: contribution of the σ54 regulon

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    da Silva Neto José F

    2010-08-01

    Full Text Available Abstract Background Xylella fastidiosa, a Gram-negative fastidious bacterium, grows in the xylem of several plants causing diseases such as citrus variegated chlorosis. As the xylem sap contains low concentrations of amino acids and other compounds, X. fastidiosa needs to cope with nitrogen limitation in its natural habitat. Results In this work, we performed a whole-genome microarray analysis of the X. fastidiosa nitrogen starvation response. A time course experiment (2, 8 and 12 hours of cultures grown in defined medium under nitrogen starvation revealed many differentially expressed genes, such as those related to transport, nitrogen assimilation, amino acid biosynthesis, transcriptional regulation, and many genes encoding hypothetical proteins. In addition, a decrease in the expression levels of many genes involved in carbon metabolism and energy generation pathways was also observed. Comparison of gene expression profiles between the wild type strain and the rpoN null mutant allowed the identification of genes directly or indirectly induced by nitrogen starvation in a σ54-dependent manner. A more complete picture of the σ54 regulon was achieved by combining the transcriptome data with an in silico search for potential σ54-dependent promoters, using a position weight matrix approach. One of these σ54-predicted binding sites, located upstream of the glnA gene (encoding glutamine synthetase, was validated by primer extension assays, confirming that this gene has a σ54-dependent promoter. Conclusions Together, these results show that nitrogen starvation causes intense changes in the X. fastidiosa transcriptome and some of these differentially expressed genes belong to the σ54 regulon.

  11. N-acetylcysteine in agriculture, a novel use for an old molecule: focus on controlling the plant-pathogen Xylella fastidiosa.

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    Lígia S Muranaka

    Full Text Available Xylella fastidiosa is a plant pathogen bacterium that causes diseases in many different crops. In citrus, it causes Citrus Variegated Chlorosis (CVC. The mechanism of pathogenicity of this bacterium is associated with its capacity to colonize and form a biofilm in the xylem vessels of host plants, and there is not yet any method to directly reduce populations of this pathogen in the field. In this study, we investigated the inhibitory effect of N-Acetylcysteine (NAC, a cysteine analogue used mainly to treat human diseases, on X. fastidiosa in different experimental conditions. Concentrations of NAC over 1 mg/mL reduced bacterial adhesion to glass surfaces, biofilm formation and the amount of exopolysaccharides (EPS. The minimal inhibitory concentration of NAC was 6 mg/mL. NAC was supplied to X. fastidiosa-infected plants in hydroponics, fertigation, and adsorbed to organic fertilizer (NAC-Fertilizer. HPLC analysis indicated that plants absorbed NAC at concentrations of 0.48 and 2.4 mg/mL but not at 6 mg/mL. Sweet orange plants with CVC symptoms treated with NAC (0.48 and 2.4 mg/mL in hydroponics showed clear symptom remission and reduction in bacterial population, as analyzed by quantitative PCR and bacterial isolation. Experiments using fertigation and NAC-Fertilizer were done to simulate a condition closer to that normally is used in the field. For both, significant symptom remission and a reduced bacterial growth rate were observed. Using NAC-Fertilizer the lag for resurgence of symptoms on leaves after interruption of the treatment increased to around eight months. This is the first report of the anti-bacterial effect of NAC against a phytopathogenic bacterium. The results obtained in this work together with the characteristics of this molecule indicate that the use of NAC in agriculture might be a new and sustainable strategy for controlling plant pathogenic bacteria.

  12. Caracterização da comunidade bacteriana endofítica de citros por isolamento, PCR específico e DGGE Characterization of the endophytic bacterial community from citrus by isolation, specific PCR and DGGE

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    Paulo Teixeira Lacava

    2006-04-01

    Full Text Available O objetivo deste trabalho foi caracterizar a comunidade bacteriana endofítica de plantas assintomáticas (escapes e afetadas pela clorose variegada dos citros (CVC por meio de isolamento em meio de cultura, técnica de gradiente desnaturante em gel de eletroforese (DGGE e detecção de Methylobacterium mesophilicum e Xyllela fastidiosa por meio de PCR específico, para estudar esta comunidade e sua relação com a ocorrência da CVC. A análise da comunidade bacteriana via DGGE permitiu a detecção de X. fastidiosa, bem como Klebsiella sp. e Acinetobacter sp. como endófitos de citros. Foram observados também Curtobacterium sp., Pseudomonas sp., Enterobacter sp. e Bacillus spp. Utilizando primers específicos, Methylobacterium mesophilicum e X. fastidiosa também foram observadas, reforçando hipóteses de que estas bactérias podem estar interagindo no interior da planta hospedeira.The aim of this work was to characterize endophytic bacterial community of assintomatic (escape and Citrus Variegated Chlorosis (CVC-affected citrus plants using isolation in culture medium, denaturing gradient gel electrophoresis (DGGE technique and Methylobacterium mesophilicum as well as Xylella fastidiosa specific PCR, allowing to assess this community and its interactions with CVC. The study of bacterial community by DGGE analysis allowed the detection of X. fastidiosa, as well as Klebsiella sp. e Acinetobacter sp., which were not detected previously. Curtobacterium sp., Pseudomonas sp., Enterobacter sp. and Bacillus spp. were also observed as endophyte in citrus plants. Using specific primers Methylobacterium mesophilicum and X. fastidiosa were observed, reinforcing that these bacteria could interact inside the host plant.

  13. Study on Spheroidization and Related Heat Treatments of Medium Carbon Alloy Steels

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    Harisha S. R.

    2018-01-01

    Full Text Available The importance of medium carbon steels as engineering materials is reflected by the fact that out of the vast majority of engineering grade ferrous alloys available and used in the market today, a large proportion of them are from the family of medium carbon steels. Typically medium carbon steels have a carbon range of 0.25 to 0.65% by weight, and a manganese content ranging from 0.060 to 1.65% by weight. Medium carbon steels are more resistive to cutting, welding and forming as compared to low carbon steels. From the last two decades a number of research scholars reported the use of verity of heat treatments to tailor the properties of medium carbon steels. Spheroidizing is the novel industrial heat treatment employed to improve formability and machinability of medium/high carbon low alloy steels. This exclusive study covers procedure, the effects and possible outcomes of various heat treatments on medium carbon steels. In the present work, other related heat treatments like annealing and special treatments for property alterations which serve as pretreatments for spheroidizing are also reviewed. Medium carbon steels with property alterations by various heat treatment processes are finding increased responsiveness in transportation, aerospace, space, underwater along with other variegated fields. Improved tribological and mechanical properties consisting of impact resistance, stiffness, abrasion and strength are the main reasons for the increased attention of these steels in various industries. In the present scenario for the consolidation of important aspects of various heat treatments and effects on mechanical properties of medium carbons steel, a review of different research papers has been attempted. This review may be used as a guide to provide practical data for heat treatment industry, especially as a tool to enhance workability and tool life.

  14. A History of the Discovery of Random X Chromosome Inactivation in the Human Female and its Significance

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    Sophia Balderman

    2011-07-01

    Full Text Available Genetic determinants of sex in placental mammals developed by the evolution of primordial autosomes into the male and female sex chromosomes. The Y chromosome determines maleness by the action of the gene SRY, which encodes a protein that initiates a sequence of events prompting the embryonic gonads to develop into testes. The X chromosome in the absence of a Y chromosome results in a female by permitting the conversion of the embryonic gonads into ovaries. We trace the historical progress that resulted in the discovery that one X chromosome in the female is randomly inactivated in early embryogenesis, accomplishing approximate equivalency of X chromosome gene dosage in both sexes. This event results in half of the somatic cells in a tissue containing proteins encoded by the genes of the maternal X chromosome and half having proteins encoded by the genes of the paternal X chromosome, on average, accounting for the phenotype of a female heterozygote with an X chromosome mutation. The hypothesis of X chromosome inactivation as a random event early in embryogenesis was first described as a result of studies of variegated coat color in female mice. Similar results were found in women using the X chromosome-linked gene, glucose-6-phosphate dehydrogenase, studied in red cells. The random inactivation of the X chromosome-bearing genes for isoenzyme types A and B of glucose-6-phosphate dehydrogenase was used to establish the clonal origin of neoplasms in informative women with leiomyomas. Behind these discoveries are the stories of the men and women scientists whose research enlightened these aspects of X chromosome function and their implication for medicine.

  15. Medications development for substance-use disorders: contextual influences (dis)incentivizing pharmaceutical-industry positioning.

    Science.gov (United States)

    Janero, David R

    2014-11-01

    The significant contribution of substance-use disorders (SUDs) to the global-disease burden and associated unmet medical needs has not engendered a commensurate level of pharma-industry research and development (R&D) for novel SUD therapeutics invention. Analysis of contextual factors shaping this position suggests potential routes toward incentivizing R&D commitment for that purpose. This article considers multiple primary factors that have consorted to disincentivize pharma industry's operating in the SUD space: ill-understood pathology; variegated treatments and patient profiles; involved clinical trials; and - with particular reference to SUDs-negative cultural/business stigmas and shallow commercial precedent. Industry incentivization for SUD drug innovation requires progress on several fronts, including: translational experimental data and systems; personalized, holistic SUD treatment approaches; interactions among pharma, nonindustry constituencies, and the medical profession with vested interests in countering negative stereotypes and expanding SUD treatment options; and public-private alliances focused on improving SUD pharmacotherapy. Given the well-entrenched business stance whereby the prospect of future profits in major markets largely determines drug-company R&D investment trajectory, strategic initiatives offering substantial reductions in the risks and opportunity (i.e., time and money) costs associated with SUD drug discovery are likely to be the most potent drivers for encouraging mainstream industry positioning in this therapeutic area. Such initiatives could originate from front-loaded R&D operational and back-loaded patent, regulatory, marketing and health-care policy reforms. These may be too involved and protracted for the turbulent pharmaceutical industry to entertain amid its recent retrenchment from psychiatric/CNS diseases and intense pressures to increase productivity and shareholder value.

  16. Species composition of insects in contaminated areas of Apsheron

    International Nuclear Information System (INIS)

    Guseynzadeh, G.A; Mamedova, T.G; Atakishiyeva, A.M; Hajiyeva, S.A

    2010-01-01

    Full text:The main area of the Apsheron Peninsula, semi-complete urbanization area, where as a result of human impact, in particular oil production and processing large areas of once fertile land have been ruined. As a result, formed locally contaminated sites where the oil soaks into the soil to a depth of 1m or more, the mold is completely ruined. The aim of our research was to study the species composition of bugs, beetles, butterflies, bees in these locally contaminated areas of Absheron, their relationships and nutritive degree of occurrence. Expeditionary trips were made in the following areas: Karadag, Seaside, Bibi Geybatsky, Surakhani, Sabunchu Oil Gas Mining Management, Ramaninsky iodine plant. These areas are divided into three gradations of anthropogenic strain:1) The weak degree of load-zone relatively clean; 2) The average degree of load-space of moderate economic use; A strong degree of load-immediately adjacent to the locally-contaminated sites. According to the results of environmental monitoring of terrestrial invertebrates group found: bugs, 22 species, 10 species of beetles, butterflies, eight species of bee-25, found food and the degree of occurrence of communication. Identified several types of bee-bioindicators: Zholletes similes, Schylaeus variegate, Andrew Flavipes. Identification of these species will provide an opportunity to determine in advance the impact of background radiation in this ecosystem and to conduct cleaning, remediation work. On the basis of faunal material collected in Table 1 shows the species composition of the above insects, food relations and the degree of occurrence.

  17. Isolation of Xylella fastidiosa from Citrus sinensis (L) Osb. And Vitis vinifera and study of genetic diversity in Costa Rica

    International Nuclear Information System (INIS)

    Aguilar Alvarez, Estela Yamileth

    2007-01-01

    Xylella fastidiosa is a plant pathogenic bacterium that causes diseases in different crops. Symptoms similar to those caused by citrus variegated chlorosis (CVC) were observed in sweet orange trees which served as shade and fences in coffee plantations in Costa Rica, in 2002. A total of 35 citrus trees and 24 vines from eight different districts and 3 respectively were evaluated by 'double antibody sandwich enzyme-linked immunosorbent assay' (DASELISA), resulting in 21 citrus and 19 positive vid. From four citrus trees and six of vines, were obtained six isolates and seven isolates respectively in solid medium, whose morphological and biochemical characteristics coincided with those reported in the literature as characteristic of X. fastidiosa. The identity of the isolates is confirmed by the chain polymerase reaction (PCR) using primers 272-1/272-2int and RST31/RST33. Three isolates from Grecia (Alajuela Province) amplified a band of 500pb using specific primers 272-2int/CVC-1 for strains of X. fastidiosa that cause CVC. The genetic variability of isolates from each other in comparison with isolates of coffee in Costa Rica, U.S. grapes and citrus in Brazil have been studied using techniques of random amplification polymorphism DNA (RAPD) and length polymorphisms of restriction fragments (RFLPs) of the products obtained with primers int/272-2int JB-1/JB-2 and 272-1. The results showed a clear separation between citrus isolates of Costa Rica; and, an association of three of them with the strains of citrus in Brasil. Also, an association between strains of coffee of Costa Rica with grape vines in the U.S. An association of molecular analysis confirmed the data variance. (author) [es

  18. Radar observations and shape model of asteroid 16 Psyche

    Science.gov (United States)

    Shepard, Michael K.; Richardson, James; Taylor, Patrick A.; Rodriguez-Ford, Linda A.; Conrad, Al; de Pater, Imke; Adamkovics, Mate; de Kleer, Katherine; Males, Jared R.; Morzinski, Katie M.; Close, Laird M.; Kaasalainen, Mikko; Viikinkoski, Matti; Timerson, Bradley; Reddy, Vishnu; Magri, Christopher; Nolan, Michael C.; Howell, Ellen S.; Benner, Lance A. M.; Giorgini, Jon D.; Warner, Brian D.; Harris, Alan W.

    2017-01-01

    Using the S-band radar at Arecibo Observatory, we observed 16 Psyche, the largest M-class asteroid in the main belt. We obtained 18 radar imaging and 6 continuous wave runs in November and December 2015, and combined these with 16 continuous wave runs from 2005 and 6 recent adaptive-optics (AO) images (Drummond et al., 2016) to generate a three-dimensional shape model of Psyche. Our model is consistent with a previously published AO image (Hanus et al., 2013) and three multi-chord occultations. Our shape model has dimensions 279 × 232 × 189 km (± 10%), Deff = 226 ± 23 km, and is 6% larger than, but within the uncertainties of, the most recently published size and shape model generated from the inversion of lightcurves (Hanus et al., 2013). Psyche is roughly ellipsoidal but displays a mass-deficit over a region spanning 90° of longitude. There is also evidence for two ∼50-70 km wide depressions near its south pole. Our size and published masses lead to an overall bulk density estimate of 4500 ± 1400 kgm-3. Psyche's mean radar albedo of 0.37 ± 0.09 is consistent with a near-surface regolith composed largely of iron-nickel and ∼40% porosity. Its radar reflectivity varies by a factor of 1.6 as the asteroid rotates, suggesting global variations in metal abundance or bulk density in the near surface. The variations in radar albedo appear to correlate with large and small-scale shape features. Our size and Psyche's published absolute magnitude lead to an optical albedo of pv = 0.15 ± 0.03, and there is evidence for albedo variegations that correlate with shape features.

  19. Asteroid 16 Psyche: Radar Observations and Shape Model

    Science.gov (United States)

    Shepard, Michael K.; Richardson, James E.; Taylor, Patrick A.; Rodriguez-Ford, Linda A.; Conrad, Al; de Pater, Imke; Adamkovics, Mate; de Kleer, Katherine R.; Males, Jared; Morzinski, Kathleen M.; Miller Close, Laird; Kaasalainen, Mikko; Viikinkoski, Matti; Timerson, Bradley; Reddy, Vishnu; Magri, Christopher; Nolan, Michael C.; Howell, Ellen S.; Warner, Brian D.; Harris, Alan W.

    2016-10-01

    We observed 16 Psyche, the largest M-class asteroid in the main belt, using the S-band radar at Arecibo Observatory. We obtained 18 radar imaging and 6 continuous wave runs in November and December 2015, and combined these with 16 continuous wave runs from 2005 and 6 recent adaptive-optics (AO) images to generate a three-dimensional shape model of Psyche. Our model is consistent with a previously published AO image [Hanus et al. Icarus 226, 1045-1057, 2013] and three multi-chord occultations. Our shape model has dimensions 279 x 232 x 189 km (±10%), Deff = 226 ± 23 km, and is 6% larger than, but within the uncertainties of, the most recently published size and shape model generated from the inversion of lightcurves [Hanus et al., 2013]. Psyche is roughly ellipsoidal but displays a mass-deficit over a region spanning 90° of longitude. There is also evidence for two ~50-70 km wide depressions near its south pole. Our size and published masses lead to an overall bulk density estimate of 4500 ± 1400 kg m-3. Psyche's mean radar albedo of 0.37 ± 0.09 is consistent with a near-surface regolith composed largely of iron-nickel and ~40% porosity. Its radar reflectivity varies by a factor of 1.6 as the asteroid rotates, suggesting global variations in metal abundance or bulk density in the near surface. The variations in radar albedo appear to correlate with large and small-scale shape features. Our size and Psyche's published absolute magnitude lead to an optical albedo of pv = 0.15 ± 0.03, and there is evidence for albedo variegations that correlate with shape features.

  20. Atmospheric reaction systems as null-models to identify structural traces of evolution in metabolism.

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    Petter Holme

    Full Text Available The metabolism is the motor behind the biological complexity of an organism. One problem of characterizing its large-scale structure is that it is hard to know what to compare it to. All chemical reaction systems are shaped by the same physics that gives molecules their stability and affinity to react. These fundamental factors cannot be captured by standard null-models based on randomization. The unique property of organismal metabolism is that it is controlled, to some extent, by an enzymatic machinery that is subject to evolution. In this paper, we explore the possibility that reaction systems of planetary atmospheres can serve as a null-model against which we can define metabolic structure and trace the influence of evolution. We find that the two types of data can be distinguished by their respective degree distributions. This is especially clear when looking at the degree distribution of the reaction network (of reaction connected to each other if they involve the same molecular species. For the Earth's atmospheric network and the human metabolic network, we look into more detail for an underlying explanation of this deviation. However, we cannot pinpoint a single cause of the difference, rather there are several concurrent factors. By examining quantities relating to the modular-functional organization of the metabolism, we confirm that metabolic networks have a more complex modular organization than the atmospheric networks, but not much more. We interpret the more variegated modular arrangement of metabolism as a trace of evolved functionality. On the other hand, it is quite remarkable how similar the structures of these two types of networks are, which emphasizes that the constraints from the chemical properties of the molecules has a larger influence in shaping the reaction system than does natural selection.

  1. The Effect of Human Development on Mammal Populations of the Punta Leona Private Wildlife Refuge, Costa Rica

    Directory of Open Access Journals (Sweden)

    Michael Van Hulle

    2009-06-01

    Full Text Available The effect of human development on six diurnal mammal species was studied using transects in the Punta Leona Private Wildlife Refuge, Puntarenas, Costa Rica during the dry season months of March and April 2006. Individuals/km was recorded for each species in more developed (MD (near paved roads, buildings, construction, or deforested trees and less developed areas (LD (secondary forest. The white-faced apuchin monkey (Cebus capucinus (n = 233, coatimundi (Nasua narica (n = 46, and Central American spider monkey (Ateles geoffroyi (n = 36 demonstrated a preference for less-developed habitats. The nine-banded armadillo (Dasypus novemcinctus (n = 4, tamandua (Tamandua mexicana (n = 2 and variegated squirrel (Sciurus variegatoides (n = 5 were observed infrequently. White-faced monkeys avoided construction areas, but received artificial food daily in developed areas. Coatimundis also received artificial foods daily and showed aggression towards guests. Rev. Biol. Trop. 57 (1-2: 441-449. Epub 2009 June 30.Se estudiaron seis especies de mamíferos mediante transectos durante la estación seca tardía (marzo y abril de 1996 en el Refugio de Vida Silvestre de Punta Leona, Puntarenas, Costa Rica. Se registró la cantidad de animales por km en lugares con mucho efecto humano (cerca del caminos pavimentados, edificios, construcción, y deforestación y lugares con menos efecto. El mono carablanca (Cebus capucinus (n = 233, el pizote (Nasua narica (n = 46, y el mono araña centroamericano (Ateles geoffroyi (n = 36 prefirieron lugares con menos efecto. Se observó pocos armadillos (Dasypus novemcinctus (n = 4, osos hormigueros (Tamandua mexicana (n = 2, y ardillas (Sciurus variegatoides (n = 5. Los monos carablancas y pizotes aceptan alimentos artificiales y los pizotes fueron agresivos con los turistas. Los carablancas evitan los lugares con construcciones y los caminos pavimentados.

  2. Satellite Maps and Relevant Compositional Properties of PM2.5 in Difficult Winter Situations and Comparisons to DISCOVER-AQ Airborne Sampling

    Science.gov (United States)

    Chatfield, Robert B.

    2016-01-01

    Mediterranean-climate regions like California's San Joaquin Valley are subject to severe wintertime particulate pollution affecting public health. We present maps of episodes and particulate diagnostics to aid diagnosis and amelioration. For abatement at sources, we require an understanding of sources and transport. Remote sensing should be of aid, but radiance-to-particle relationships are far different from methods which have been of use in the Eastern USA, Northern and Central Europe. Here are the problems: (a) Thin if very polluted mixed layers (MLs) yield optical depths, AOD, near the detection level, (b) bright and quite variegated surfaces (c) Unusual particle composition (e.g., predominance of NH4NO3 and fireplace buning aerosol), which complicate the relationship of AOD to PM2.5. Specialized analysis of MODIS-Aqua data to obtain AOD using the multi-angle (MAIAC) technique employed by Lyapustin and Wang. Meteorological analyses like NOAA's Rapid Analysis Product (RAP, or newer products like HRRR), which are formulated to remain close to observations (e.g. of water), provide useful ML information corroborated by DISCOVER-AQ in-situ and lidar observations. The many PM2.5 measurements allow a calibration of these products and thus maps of aerosol on many successive aerosol buildups. These calibrations also allow insight into compositional information relevant to MODIS retrievals, the product of aerosol density and specific scattering. We have found that the rich in-situ, lidar, and sun-photometer data sets of NASA'S DISCOVER-AQ data set (2013) of great aid. We will highlight the most interesting of many intercomparisons possible with this rich data set. We conclude with a description of new work to extend these insights to similar regions, e.g. the Imperial Valley of California, the Po Valley and maritime Southern Europe, and the litoral regions of Israel.

  3. Drosophila larvae synthesize the putative oncometabolite L-2-hydroxyglutarate during normal developmental growth.

    Science.gov (United States)

    Li, Hongde; Chawla, Geetanjali; Hurlburt, Alexander J; Sterrett, Maria C; Zaslaver, Olga; Cox, James; Karty, Jonathan A; Rosebrock, Adam P; Caudy, Amy A; Tennessen, Jason M

    2017-02-07

    L-2-hydroxyglutarate (L-2HG) has emerged as a putative oncometabolite that is capable of inhibiting enzymes involved in metabolism, chromatin modification, and cell differentiation. However, despite the ability of L-2HG to interfere with a broad range of cellular processes, this molecule is often characterized as a metabolic waste product. Here, we demonstrate that Drosophila larvae use the metabolic conditions established by aerobic glycolysis to both synthesize and accumulate high concentrations of L-2HG during normal developmental growth. A majority of the larval L-2HG pool is derived from glucose and dependent on the Drosophila estrogen-related receptor (dERR), which promotes L-2HG synthesis by up-regulating expression of the Drosophila homolog of lactate dehydrogenase (dLdh). We also show that dLDH is both necessary and sufficient for directly synthesizing L-2HG and the Drosophila homolog of L-2-hydroxyglutarate dehydrogenase (dL2HGDH), which encodes the enzyme that breaks down L-2HG, is required for stage-specific degradation of the L-2HG pool. In addition, dLDH also indirectly promotes L-2HG accumulation via synthesis of lactate, which activates a metabolic feed-forward mechanism that inhibits dL2HGDH activity and stabilizes L-2HG levels. Finally, we use a genetic approach to demonstrate that dLDH and L-2HG influence position effect variegation and DNA methylation, suggesting that this compound serves to coordinate glycolytic flux with epigenetic modifications. Overall, our studies demonstrate that growing animal tissues synthesize L-2HG in a controlled manner, reveal a mechanism that coordinates glucose catabolism with L-2HG synthesis, and establish the fly as a unique model system for studying the endogenous functions of L-2HG during cell growth and proliferation.

  4. A chemical specialty semantic network for the Unified Medical Language System

    Directory of Open Access Journals (Sweden)

    Morrey C

    2012-05-01

    Full Text Available Abstract Background Terms representing chemical concepts found the Unified Medical Language System (UMLS are used to derive an expanded semantic network with mutually exclusive semantic types. The UMLS Semantic Network (SN is composed of a collection of broad categories called semantic types (STs that are assigned to concepts. Within the UMLS’s coverage of the chemical domain, we find a great deal of concepts being assigned more than one ST. This leads to the situation where the extent of a given ST may contain concepts elaborating variegated semantics. A methodology for expanding the chemical subhierarchy of the SN into a finer-grained categorization of mutually exclusive types with semantically uniform extents is presented. We call this network a Chemical Specialty Semantic Network (CSSN. A CSSN is derived automatically from the existing chemical STs and their assignments. The methodology incorporates a threshold value governing the minimum size of a type’s extent needed for inclusion in the CSSN. Thus, different CSSNs can be created by choosing different threshold values based on varying requirements. Results A complete CSSN is derived using a threshold value of 300 and having 68 STs. It is used effectively to provide high-level categorizations for a random sample of compounds from the “Chemical Entities of Biological Interest” (ChEBI ontology. The effect on the size of the CSSN using various threshold parameter values between one and 500 is shown. Conclusions The methodology has several potential applications, including its use to derive a pre-coordinated guide for ST assignments to new UMLS chemical concepts, as a tool for auditing existing concepts, inter-terminology mapping, and to serve as an upper-level network for ChEBI.

  5. The role of private organisations in welfare work. The historical perspective.

    Science.gov (United States)

    Hastrup, B

    1992-06-01

    The Danish welfare measures originated in private initiatives. From the end of the last century, welfare activities have developed through private relief organisations and with support from the State, local governments, and counties. Since the 1960s, many institutions which had been built up by private relief organisations were actually taken over by local governments or counties. By way of illustration, the development of EGV DaneCare (the Danish Association for the Care of the Elderly), from its origin as a voluntary relief organisation based on voluntary work and collected funds, is described. From 1910, EGV was the pioneer within the field of, for example, old people's homes, winter shelters, and holidays in the country for elderly people. In the 1960s, EGV became a professional service organisation which assisted the local governments in developing a wide range of variegated offers within the work for elderly people. Assistance offered to the local governments became the organisation's principal project up to the 1980s. The population's confidence in the ability of the public authorities to cope with the social tasks was at the time undermined by increasing scepticism. That was why EGV founded the DaneAge Association, which soon became a national cause with more than 200,000 members. The DaneAge Association operates as an association independent of the public authorities pursuing both local and national policies concerning the elderly and implementing humanitarian initiatives based on voluntary work. Studies made by the DaneAge Association show, among other things, that 20 per cent of the people aged 60 and over need practical assistance.(ABSTRACT TRUNCATED AT 250 WORDS)

  6. Silencing inhibits Cre-mediated recombination of the Z/AP and Z/EG reporters in adult cells.

    Directory of Open Access Journals (Sweden)

    Michael A Long

    Full Text Available BACKGROUND: The Cre-loxP system has been used to enable tissue specific activation, inactivation and mutation of many genes in vivo and has thereby greatly facilitated the genetic dissection of several cellular and developmental processes. In such studies, Cre-reporter strains, which carry a Cre-activated marker gene, are frequently utilized to validate the expression profile of Cre transgenes, to act as a surrogate marker for excision of a second allele, and to irreversibly label cells for lineage tracing experiments. PRINCIPAL FINDINGS: We have studied three commonly used Cre-reporter strains, Z/AP, Z/EG and R26R-EYFP and have demonstrated that although each reporter can be reliably activated by Cre during early development, exposure to Cre in adult hematopoietic cells results in a much lower frequency of marker-positive cells in the Z/AP or Z/EG strains than in the R26R-EYFP strain. In marker negative cells derived from the Z/AP and Z/EG strains, the transgenic promoter is methylated and Cre-mediated recombination of the locus is inhibited. CONCLUSIONS: These results show that the efficiency of Cre-mediated recombination is not only dependent on the genomic context of a given loxP-flanked sequence, but also on stochastic epigenetic mechanisms underlying transgene variegation. Furthermore, our data highlights the potential shortcomings of utilizing the Z/AP and Z/EG reporters as surrogate markers of excision or in lineage tracing experiments.

  7. Drosophila larvae synthesize the putative oncometabolite L-2-hydroxyglutarate during normal developmental growth

    Science.gov (United States)

    Li, Hongde; Chawla, Geetanjali; Hurlburt, Alexander J.; Sterrett, Maria C.; Zaslaver, Olga; Cox, James; Karty, Jonathan A.; Rosebrock, Adam P.; Caudy, Amy A.

    2017-01-01

    L-2-hydroxyglutarate (L-2HG) has emerged as a putative oncometabolite that is capable of inhibiting enzymes involved in metabolism, chromatin modification, and cell differentiation. However, despite the ability of L-2HG to interfere with a broad range of cellular processes, this molecule is often characterized as a metabolic waste product. Here, we demonstrate that Drosophila larvae use the metabolic conditions established by aerobic glycolysis to both synthesize and accumulate high concentrations of L-2HG during normal developmental growth. A majority of the larval L-2HG pool is derived from glucose and dependent on the Drosophila estrogen-related receptor (dERR), which promotes L-2HG synthesis by up-regulating expression of the Drosophila homolog of lactate dehydrogenase (dLdh). We also show that dLDH is both necessary and sufficient for directly synthesizing L-2HG and the Drosophila homolog of L-2-hydroxyglutarate dehydrogenase (dL2HGDH), which encodes the enzyme that breaks down L-2HG, is required for stage-specific degradation of the L-2HG pool. In addition, dLDH also indirectly promotes L-2HG accumulation via synthesis of lactate, which activates a metabolic feed-forward mechanism that inhibits dL2HGDH activity and stabilizes L-2HG levels. Finally, we use a genetic approach to demonstrate that dLDH and L-2HG influence position effect variegation and DNA methylation, suggesting that this compound serves to coordinate glycolytic flux with epigenetic modifications. Overall, our studies demonstrate that growing animal tissues synthesize L-2HG in a controlled manner, reveal a mechanism that coordinates glucose catabolism with L-2HG synthesis, and establish the fly as a unique model system for studying the endogenous functions of L-2HG during cell growth and proliferation. PMID:28115720

  8. An Evaluation of the Genetic Diversity of Xylella fastidiosa Isolated from Diseased Citrus and Coffee in São Paulo, Brazil.

    Science.gov (United States)

    Qin, X; Miranda, V S; Machado, M A; Lemos, E G; Hartung, J S

    2001-06-01

    ABSTRACT Strains of Xylella fastidiosa, isolated from sweet orange trees (Citrus sinensis) and coffee trees (Coffea arabica) with symptoms of citrus variegated chlorosis and Requeima do Café, respectively, were indistinguishable based on repetitive extragenic palindromic polymerase chain reaction (PCR) and enterobacterial repetitive intergenic consensus PCR assays. These strains were also indistinguishable with a previously described PCR assay that distinguished the citrus strains from all other strains of Xylella fastidiosa. Because we were not able to document any genomic diversity in our collection of Xylella fastidiosa strains isolated from diseased citrus, the observed gradient of increasing disease severity from southern to northern regions of São Paulo State is unlikely due to the presence of significantly different strains of the pathogen in the different regions. When comparisons were made to reference strains of Xylella fastidiosa isolated from other hosts using these methods, four groups were consistently identified consistent with the hosts and regions from which the strains originated: citrus and coffee, grapevine and almond, mulberry, and elm, plum, and oak. Independent results from random amplified polymorphic DNA (RAPD) PCR assays were also consistent with these results; however, two of the primers tested in RAPD-PCR were able to distinguish the coffee and citrus strains. Sequence comparisons of a PCR product amplified from all strains of Xylella fastidiosa confirmed the presence of a CfoI polymorphism that can be used to distinguish the citrus strains from all others. The ability to distinguish Xylella fastidiosa strains from citrus and coffee with a PCR-based assay will be useful in epidemiological and etiological studies of this pathogen.

  9. The endophyte Curtobacterium flaccumfaciens reduces symptoms caused by Xylella fastidiosa in Catharanthus roseus.

    Science.gov (United States)

    Lacava, Paulo Teixeira; Li, Wenbin; Araújo, Welington Luiz; Azevedo, João Lúcio; Hartung, John Stephen

    2007-10-01

    Citrus variegated chlorosis (CVC) is a disease of the sweet orange [Citrus sinensis (L.)], which is caused by Xylella fastidiosa subsp. pauca, a phytopathogenic bacterium that has been shown to infect all sweet orange cultivars. Sweet orange trees have been occasionally observed to be infected by Xylella fastidiosa without evidencing severe disease symptoms, whereas other trees in the same grove may exhibit severe disease symptoms. The principal endophytic bacterial species isolated from such CVC-asymptomatic citrus plants is Curtobacterium flaccumfaciens. The Madagascar periwinkle [Citrus sinensis (L.)] is a model plant which has been used to study X. fastidiosa in greenhouse environments. In order to characterize the interactions of X. fastidiosa and C. flaccumfaciens, periwinkle plants were inoculated separately with C. flaccumfaciens, X. fastidiosa, and both bacteria together. The number of flowers produced by the plants, the heights of the plants, and the exhibited disease symptoms were evaluated. PCR-primers for C. flaccumfaciens were designed in order to verify the presence of this endophytic bacterium in plant tissue, and to complement an existing assay for X. fastidiosa. These primers were capable of detecting C. flaccumfaciens in the periwinkle in the presence of X. fastidiosa. X. fastidiosa induced stunting and reduced the number of flowers produced by the periwinkle. When C. flaccumfaciens was inoculated together with X. fastidiosa, no stunting was observed. The number of flowers produced by our doubly- inoculated plants was an intermediate between the number produced by the plants inoculated with either of the bacteria separately. Our data indicate that C. flaccumfaciens interacted with X. fastidiosa in C. roseus, and reduced the severity of the disease symptoms induced by X. fastidiosa. Periwinkle is considered to be an excellent experimental system by which the interaction of C. flaccumfaciens and other endophytic bacteria with X. fastidiosa can be

  10. Gamma background irradiation. Standards and reality

    International Nuclear Information System (INIS)

    Miloslavov, V.

    1998-01-01

    The systematic deviation of the results of measuring the power of air dose absorbed from the natural gamma background radiation in Bulgaria is inadmissibly large and variable. This in turn augments the dispersion of results as well as the mean value relative to worldwide data, to an implausible level, hardly attributable to the variegated geographical relief of the country. Thus in practice local anthropogenic increases hardly lend themselves to detection and demonstration. In the Radiation Protection Standards (RPS-92) in effect in Bulgaria, and in other documents concerning the same radiation factors as well, the maximum allowable limits for the population as a whole are clearly specified on the basis of worldwide expertise along this line. As a rule these limits are being exceeded by the actually measured values, and for this reason the cited documents contain a clause stipulating that these limits do not refer to the natural radiation background and therefore the latter may be virtually ignored. Thus the basic risk factor for the population goes beyond control at levels commensurable with the officially established limits, its twofold increase inclusive. The maximum allowable limit becomes undefinable. Bearing in mind the fact that in compliance with the cited RPS-92 elimination of the technogenic ionizing radiation sources incorporated in the environment prior to 1992 is 'freezed', it is evident that exposure of the population to anthropogenic radiation becomes legally allowable in a much wider range than the one specified by world legislators. One may anticipate radiation induced health noxae for the population directly or by anthropogenic radiation stress on biocenosis. A relatively large part of the population is susceptible to the effect of low radiation doses. Presumably this contingent will augment as a result of eventual fluctuations. The casual relationship which is difficult to establish should be given due consideration in the analysis of the causes

  11. Ultrasound assisted enzymatic hydrolysis for isolating titanium dioxide nanoparticles from bivalve mollusk before sp-ICP-MS.

    Science.gov (United States)

    Taboada-López, María Vanesa; Iglesias-López, Sara; Herbello-Hermelo, Paloma; Bermejo-Barrera, Pilar; Moreda-Piñeiro, Antonio

    2018-08-14

    Applicability of single-particle inductively coupled plasma mass spectrometry (sp-ICP-MS) using dwell times equal to or shorter than 100 μs has been tested for assessing titanium dioxide nanoparticles (TiO 2 NPs) in bivalve mollusks. TiO 2 NPs isolation from fresh mollusk tissues was achieved by ultrasound assisted enzymatic hydrolysis procedure using a pancreatin/lipase mixture. Optimum extraction conditions imply ultrasonication (60% amplitude) for 10 min, and 7.5 mL of a solution containing 3.0 g L -1 of pancreatin and lipase (pH 7.4). The developed method was found to be repeatable (repeatability of 17% for the over-all procedure, TiO 2 NPs concentration of 5.33 × 10 7  ± 8.89 × 10 6 , n = 11), showing a limit of detection of 5.28 × 10 6 NPs g -1 , and a limit of detection in size of 24.4-30.4 nm, based on the 3σ criteria, and on the 3σ/5 σ criteria, respectively. The analytical recovery within the 90-99% range (use of TiO 2 NPs standards of 50 nm at 7 and 14 μg L -1 as Ti). Several bivalve mollusks (clams, cockles, mussels, razor clams, oysters and variegated scallops) were analyzed for total titanium (ICP-MS after microwave assisted acid digestion), and for TiO 2 NPs by the proposed method. TiO 2 NPs concentrations were within the 2.36 × 10 7 -1.25 × 10 8 NPs g -1 range, and the most frequent sizes were from 50 to 70 nm. Copyright © 2018 Elsevier B.V. All rights reserved.

  12. Astrocyte physiopathology: At the crossroads of intercellular networking, inflammation and cell death.

    Science.gov (United States)

    Rossi, Daniela

    2015-07-01

    Recent breakthroughs in neuroscience have led to the awareness that we should revise our traditional mode of thinking and studying the CNS, i.e. by isolating the privileged network of "intelligent" synaptic contacts. We may instead need to contemplate all the variegate communications occurring between the different neural cell types, and centrally involving the astrocytes. Basically, it appears that a single astrocyte should be considered as a core that receives and integrates information from thousands of synapses, other glial cells and the blood vessels. In turn, it generates complex outputs that control the neural circuitry and coordinate it with the local microcirculation. Astrocytes thus emerge as the possible fulcrum of the functional homeostasis of the healthy CNS. Yet, evidence indicates that the bridging properties of the astrocytes can change in parallel with, or as a result of, the morphological, biochemical and functional alterations these cells undergo upon injury or disease. As a consequence, they have the potential to transform from supportive friends and interactive partners for neurons into noxious foes. In this review, we summarize the currently available knowledge on the contribution of astrocytes to the functioning of the CNS and what goes wrong in various pathological conditions, with a particular focus on Amyotrophic Lateral Sclerosis, Alzheimer's Disease and ischemia. The observations described convincingly demonstrate that the development and progression of several neurological disorders involve the de-regulation of a finely tuned interplay between multiple cell populations. Thus, it seems that a better understanding of the mechanisms governing the integrated communication and detrimental responses of the astrocytes as well as their impact towards the homeostasis and performance of the CNS is fundamental to open novel therapeutic perspectives. Copyright © 2015 Elsevier Ltd. All rights reserved.

  13. The First USGS Global Geologic Map of Europa

    Science.gov (United States)

    Leonard, E. J.; Patthoff, D. A.; Senske, D.; Collins, G. C.

    2017-12-01

    Understanding the global scale geology of Europa is paramount to gaining insight into the potential habitability of this icy world. To this end, work is ongoing to complete a global geological map at the scale of 1:15 million that incorporates data at all resolutions collected by the Voyager and Galileo missions. The results of this work will aid the Europa Clipper mission, now in formulation, by providing a framework for collaborative and synergistic science investigations. To understand global geologic and tectonic relations, a total of 10 geologic units have been defined. These include: Low Albedo Ridge Material (lam)—low albedo material that irregularly surrounds large (>20 km) ridge structures; Ridged plains (pr)—distributed over all latitudes and characterized by subparallel to cross-cutting ridges and troughs visible at high resolution (material (b)—linear to curvilinear zones with a distinct, abrupt albedo change from the surrounding region; Crater material (c), Continuous Crater Ejecta (ce) and Discontinuous Crater Ejecta (dce)—features associated with impact craters including the site of the impact, crater material, and the fall-out debris respectively; Low Albedo Chaos (chl), Mottled Albedo Chaos (chm) and High Albedo Chaos (chh)—disrupted terrain with a relatively uniform low albedo, patchy/variegated albedo, and uniform high albedo appearance respectively; Knobby Chaos (chk) - disrupted terrain with rough and blocky texture occurring in the high latitudes. In addition to the geologic units, our mapping also includes structural features—Ridges, Cycloids, Undifferentiated Linea, Crater Rims, Depression Margins, Dome Margins and Troughs. We also introduce a point feature (at the global scale), Microchaos, to denote small (material. The completed map will constrain the distribution of different Europa terrains and provide a general stratigraphic framework to assess the geologic history of Europa from the regional to the global scale. Here, we

  14. Evolutionary diversification of plant shikimate kinase gene duplicates.

    Directory of Open Access Journals (Sweden)

    Geoffrey Fucile

    2008-12-01

    Full Text Available Shikimate kinase (SK; EC 2.7.1.71 catalyzes the fifth reaction of the shikimate pathway, which directs carbon from the central metabolism pool to a broad range of secondary metabolites involved in plant development, growth, and stress responses. In this study, we demonstrate the role of plant SK gene duplicate evolution in the diversification of metabolic regulation and the acquisition of novel and physiologically essential function. Phylogenetic analysis of plant SK homologs resolves an orthologous cluster of plant SKs and two functionally distinct orthologous clusters. These previously undescribed genes, shikimate kinase-like 1 (SKL1 and -2 (SKL2, do not encode SK activity, are present in all major plant lineages, and apparently evolved under positive selection following SK gene duplication over 400 MYA. This is supported by functional assays using recombinant SK, SKL1, and SKL2 from Arabidopsis thaliana (At and evolutionary analyses of the diversification of SK-catalytic and -substrate binding sites based on theoretical structure models. AtSKL1 mutants yield albino and novel variegated phenotypes, which indicate SKL1 is required for chloroplast biogenesis. Extant SKL2 sequences show a strong genetic signature of positive selection, which is enriched in a protein-protein interaction module not found in other SK homologs. We also report the first kinetic characterization of plant SKs and show that gene expression diversification among the AtSK inparalogs is correlated with developmental processes and stress responses. This study examines the functional diversification of ancient and recent plant SK gene duplicates and highlights the utility of SKs as scaffolds for functional innovation.

  15. The use of geographic information system and 1860s cadastral data to model agricultural suitability before heavy mechanization. A case study from Malta.

    Science.gov (United States)

    Alberti, Gianmarco; Grima, Reuben; Vella, Nicholas C

    2018-01-01

    The present study seeks to understand the determinants of land agricultural suitability in Malta before heavy mechanization. A GIS-based Logistic Regression model is built on the basis of the data from mid-1800s cadastral maps (cabreo). This is the first time that such data are being used for the purpose of building a predictive model. The maps record the agricultural quality of parcels (ranging from good to lowest), which is represented by different colours. The study treats the agricultural quality as a depended variable with two levels: optimal (corresponding to the good class) vs. non-optimal quality (mediocre, bad, low, and lowest classes). Seventeen predictors are isolated on the basis of literature review and data availability. Logistic Regression is used to isolate the predictors that can be considered determinants of the agricultural quality. Our model has an optimal discriminatory power (AUC: 0.92). The positive effect on land agricultural quality of the following predictors is considered and discussed: sine of the aspect (odds ratio 1.42), coast distance (2.46), Brown Rendzinas (2.31), Carbonate Raw (2.62) and Xerorendzinas (9.23) soils, distance to minor roads (4.88). Predictors resulting having a negative effect are: terrain elevation (0.96), slope (0.97), distance to the nearest geological fault lines (0.09), Terra Rossa soil (0.46), distance to secondary roads (0.19) and footpaths (0.41). The model isolates a host of topographic and cultural variables, the latter related to human mobility and landscape accessibility, which differentially contributed to the agricultural suitability, providing the bases for the creation of the fragmented and extremely variegated agricultural landscape that is the hallmark of the Maltese Islands. Our findings are also useful to suggest new questions that may be posed to the more meagre evidence from earlier periods.

  16. Molecular Karyotyping and Exome Analysis of Salt-Tolerant Rice Mutant from Somaclonal Variation

    Directory of Open Access Journals (Sweden)

    Thanikarn Udomchalothorn

    2014-11-01

    Full Text Available LPT123-TC171 is a salt-tolerant (ST and drought-tolerant (DT rice line that was selected from somaclonal variation of the original Leuang Pratew 123 (LPT123 rice cultivar. The objective of this study was to identify the changes in the rice genome that possibly lead to ST and/or DT characteristics. The genomes of LPT123 and LPT123-TC171 were comparatively studied at the four levels of whole chromosomes (chromosome structure including telomeres, transposable elements, and DNA sequence changes by using next-generation sequencing analysis. Compared with LPT123, the LPT123-TC171 line displayed no changes in the ploidy level, but had a significant deficiency of chromosome ends (telomeres. The functional genome analysis revealed new aspects of the genome response to the in vitro cultivation condition, where exome sequencing revealed the molecular spectrum and pattern of changes in the somaclonal variant compared with the parental LPT123 cultivar. Mutation detection was performed, and the degree of mutations was evaluated to estimate the impact of mutagenesis on the protein functions. Mutations within the known genes responding to both drought and salt stress were detected in 493 positions, while mutations within the genes responding to only salt stress were found in 100 positions. The possible functions of the mutated genes contributing to salt or drought tolerance were discussed. It was concluded that the ST and DT characteristics in the somaclonal variegated line resulted from the base changes in the salt- and drought-responsive genes rather than the changes in chromosome structure or the large duplication or deletion in the specific region of the genome.

  17. REVITALIZATION OF THE GEOLOGICAL SITES IN THE RURAL AREAS - CASE STUDY OF THE MIECHÓW UPLAND

    Directory of Open Access Journals (Sweden)

    Małgorzata Gonera

    2017-08-01

    Full Text Available The Miechów Upland is one of the meaningful areas for study the Miocene geology. Middle Miocene (Karpatian to Early Sarmatian sediments have been recognised there. The deposits are highly variegated in regard of lithology. Brackish and fresh-water Karpatian sediments are followed by different rocks of broad marine transgression during Moravian, ended by the early Sarmatian ones. Depending on the prominent paleo relief of the Cretaceous basement, a varieties of sediments have been deposited during this time. Marly claystones, silts, sands with mudstone intercalations and detrital limestones (Rhodolith bioherms are the most common. Also the Middle Badenian sulphate evaporates are in the Miechów Upland. State of the Miocene stratotype outcrops had been checked in this area. The access to most of them is impossible cause soil and vegetation coverage within the abandoned opencasts. The only exception is the small, still working silt-pit of the local brick-yard. The solution is effective to both animate the local economy and warrant the access to stratotype section. Due to fertile Pleistocene loess soils the region is extensively use by agriculture, but touched by relevant economic and social problems. The arriving to small-scale opencast mining, accompanied with innovative manufacturing of the resources (silts, clays, sands and carbonates is proposed. Modern mining elaborates a way of working in high level of accordance with the ecological engineering principles and Corporate Social Responsibility (CSR standards so shall not generate nasty threats to the state of soils and agricultural character of the region. A development of unique, regional products on the base of local resources (e.g. unique, regional decorative stones or pottery is worth to be consider by the LEADER program activities. The initiation of innovative mining enterprises and handcraft seems to be a good strategy to improve local economy and promising solution to highlight the image

  18. Reprodução de Lytechinus variegatus (Echinodermata: Echinoidea: efeito do ciclo lunar e características da população = Reproduction of Lytechinus variegatus (Echinodermata: Echinoidea: effect of lunar cycle and population characteristics

    Directory of Open Access Journals (Sweden)

    Alexandra Mara Cruz Lage

    2011-07-01

    Full Text Available Este estudo avaliou as características reprodutivas de uma população do ouriço do mar Lytechinus variegatus e a influência do ciclo lunar na liberação de gametas. Foram realizadas oito coletas para avaliação do índice gonadossomático (IG e dos parâmetros populacionais relacionados com a reprodução. Para avaliação do efeito da Lua na liberação de gametas foram realizadas coletas em três ciclos lunares completos. Ao todo foram coletados 295 indivíduos, sendo 156 machos e 131 fêmeas (proporção 1,19:1, porém não houve diferença significativa na proporção sexual da população. O peso dos machos foisignificativamente maior que o das fêmeas, porém não houve diferença significativa no IG, peso gonadal e diâmetro entre os sexos. Com a amplitude no diâmetro dos animais coletados (5,5-8,7 cm foram estabelecidas cinco classes de tamanho. Não houve diferençassignificativas no IG entre as classes de tamanho. Houve efeito da periodicidade lunar na liberação de gametas, com menor IG na lua nova, indicando que esta ocorre no primeiro quarto lunar e em marés de sizígia.This work evaluated the spawning of sea urchin Lytechinus variegates based on lunar cycle and population characteristics. Eight monthly collections were realized to estimate the gonadosomatic index (GI and population parameters regarding reproduction. To evaluate the effect of the lunar cycle on spawning, samples were taken for each moon phase of three lunar cycles. In the entire work 295 individuals were collected, 156 males and 131 females (1.19:1 ratio. However, there was no significant difference in the sex ratio of the population. The weight of males was significantly greater than that of females. There were no differences between males and females in gonadosomatic index (GI, gonad weight and diameter. There was aneffect of the lunar periodicity on spawning, with lower GI in new moon, indicating that spawning occurs on the first lunar quarter in spring

  19. Quantification and localization of hesperidin and rutin in Citrus sinensis grafted on C. limonia after Xylella fastidiosa infection by HPLC-UV and MALDI imaging mass spectrometry.

    Science.gov (United States)

    Soares, Márcio Santos; da Silva, Danielle Fernandes; Forim, Moacir Rossi; da Silva, Maria Fátima das Graças Fernandes; Fernandes, João Batista; Vieira, Paulo Cezar; Silva, Denise Brentan; Lopes, Norberto Peporine; de Carvalho, Sérgio Alves; de Souza, Alessandra Alves; Machado, Marcos Antônio

    2015-07-01

    A high performance liquid chromatography-ultraviolet (HPLC-UV) method was developed for quantifying hesperidin and rutin levels in leaves and stems of Citrus limonia, with a good linearity over a range of 1.0-80.0 and 1.0-50.0 μg mL(-1) respectively, with r(2)>0.999 for all curves. The limits of detection (LOD) for both flavonoids were 0.6 and 0.5 μg mL(-1), respectively, with quantification (LOQ) being 2.0 and 1.0 μg mL(-1), respectively. The quantification method was applied to Citrus sinensis grafted onto C. limonia with and without CVC (citrus variegated chlorosis) symptoms after Xylella fastidiosa infection. The total content of rutin was low and practically constant in all analyses in comparison with hesperidin, which showed a significant increase in its amount in symptomatic leaves. Scanning electron microscopy studies on leaves with CVC symptoms showed vessel occlusion by biofilm, and a crystallized material was noted. Considering the difficulty in isolating these crystals for analysis, tissue sections were analyzed by matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) to confirm the presence of hesperidin at the site of infection. The images constructed from MS/MS data with a specific diagnostic fragment ion (m/z 483) also showed higher ion intensities for it in infected plants than in healthy ones, mainly in the vessel regions. These data suggest that hesperidin plays a role in the plant-pathogen interaction, probably as a phytoanticipin. This method was also applied to C. sinensis and C. limonia seedlings, and comparison with the graft results showed that the rootstock had an increased hesperidin content ∼3.6 fold greater in the graft stem than in the stem of C. sinensis seedlings. Increase in hesperidin content by rootstock can be related to induced internal defense mechanisms. Copyright © 2015 Elsevier Ltd. All rights reserved.

  20. Geology of the Severnaya Zemlya Archipelago and the North Kara Terrane in the Russian high Arctic

    Science.gov (United States)

    Lorenz, Henning; Männik, Peep; Gee, David; Proskurnin, Vasilij

    2008-05-01

    The Severnaya Zemlya Archipelago is located at 80°N near the continental shelf break, between the Kara and Laptev seas. Sedimentary successions of Neoproterozoic and Palaeozoic age dominate the bedrock geology. Together with Northern Tajmyr, Severnaya Zemlya constitutes the main land areas of the North Kara Terrane (NKT), which is inferred here to have been a part of the Timanide margin of Baltica, i.e. an integral part of Baltica at least since the Vendian. Vendian turbidites derived from the Timanide Orogen are inferred to have been deposited on Neoproterozoic greenschist facies, granite-intruded basement. Shallow-water siliclastic deposition in the Early to Mid-Cambrian was followed by highly organic-rich shales in the Late Cambrian and influx of more turbidites. An episode of folding, the Kan’on River deformation, separates these formations from the overlying Tremadocian conglomerates and sandstones. In the Early Ordovician, rift-related magmatic rocks accompanied the deposition of variegated marls, sandstones, carbonates and evaporites. Dark shales and gypsiferous limestones characterise the Mid-Ordovician. Late Ordovician quartz-sandstones mark a hiatus, followed by carbonate rocks that extend up into and through most of the Silurian. The latter give way upwards into Old Red Sandstones, which are inferred to have been deposited in a Caledonian foreland basin. Deformation, reaching the area in the latest Devonian or earliest Carboniferous and referred to as the Severnaya Zemlya episode, is thought to be Caledonian-related. The dominating E-vergent structure was controlled by décollement zones in Ordovician evaporite-bearing strata; detachment folds and thrusts developed in the west and were apparently impeded by a barrier of Ordovician igneous rocks in the east. Below the décollement zones, the Neoproterozoic to Early Ordovician succession was deformed into open to close folds. The exposed strata in the lower structural level have been juxtaposed with

  1. Medium-Sized Mammals around a Radioactive Liquid Waste Lagoon at Los Alamos National Laboratory: Uptake of Contaminants and Evaluation of Radio-Frequency Identification Technology

    Energy Technology Data Exchange (ETDEWEB)

    Leslie A. Hansen; Phil R. Fresquez; Rhonda J. Robinson; John D. Huchton; Teralene S. Foxx

    1999-11-01

    Use of a radioactive liquid waste lagoon by medium-sized mammals and levels of tritium, other selected radionuclides, and metals in biological tissues of the animals were documented at Technical Area 53 (TA-53) of Los Alamos National Laboratory during 1997 and 1998. Rock squirrel (Spermophilus variegates), raccoon (Procyon lotor), striped skunk (Mephitis mephitis), and bobcat (Lynx rufus) were captured at TA-53 and at a control site on the Santa Fe National Forest. Captured animals were anesthetized and marked with radio-frequency identification (RFD) tags and/or ear tags. We collected urine and hair samples for tritium and metals (aluminum, antimony, arsenic, barium, beryllium, cadmium, chromium, copper, lead, mercury, nickel, selenium, silver, and thallium) analyses, respectively. In addition, muscle and bone samples from two rock squirrels collected from each of TA-53, perimeter, and regional background sites were tested for tritium, {sup 137}Cs, {sup 90}Sr, {sup 238}Pu, {sup 239,240}Pu, {sup 241}Am, and total uranium. Animals at TA-53 were monitored entering and leaving the lagoon area using a RFID monitor to read identification numbers from the RFID tags of marked animals and a separate camera system to photograph all animals passing through the monitor. Cottontail rabbit (Sylvilagus spp.), rock squirrel, and raccoon were the species most frequently photographed going through the RFID monitor. Less than half of all marked animals in the lagoon area were detected using the lagoon. Male and female rock squirrels from the lagoon area had significantly higher tritium concentrations compared to rock squirrels from the control area. Metals tested were not significantly higher in rock squirrels from TA-53, although there was a trend toward increased levels of lead in some individuals at TA-53. Muscle and bone samples from squirrels in the lagoon area appeared to have higher levels of tritium, total uranium, and {sup 137}Cs than samples collected from perimeter and

  2. Más poemas sefardíes de contenido histórico

    Directory of Open Access Journals (Sweden)

    Romero, Elena

    2011-06-01

    Full Text Available In addition to her book Entre dos (o más fuegos, where the author published poetical texts concerning different events in the history of the Sephardic Jews in the Ottoman Empire and the Balkans, she offers here the edition and study of more than 20 historical poems (among them, 8 “Coplas”, written by authors such as Alexandro Pereẓ, Ya‘acob Mošeh Altaraŝ, Sa‘adi Halevi, Eliyah Carmona, etc. The texts deal with a range of variegated issues, some already previously studied, and some new, among them, the bombardment of Belgrade in 1862; the Habsburg occupation of Bosnia in 1878; the fire of Sarajevo in 1879; the Sephardic community in Constantinople in 1922; Great Britain and Palestine in 1939; the Holocaust; the independence of Israel and the tough life in the new State in 1949 and in 1952; the Cold War and the Korean War in 1952; etc.

    Como complemento a su libro Entre dos (o más fuegos, donde se publicaban textos poéticos sobre diferentes acontecimientos de la historia de los sefardíes del Imperio otomano y de los Balcanes, en el presente artículo la autora edita y estudia otros 20 poemas (8 de ellos coplas de interés histórico, escritos por autores como Alexandro Péreẓ, Ya‘acob Mošé Altaráŝ, Sa‘adi Haleví, Eliyá Carmona, etc., y que abordan variados temas, algunos ya tratados en Entre dos y otros nuevos, entre los que cabe resaltar: el bombardeo de Belgrado de 1862; la ocupación de Bosnia por los austriacos en 1878; el incendio de Sarajevo de 1879; la comunidad sefardí de Constantinopla en 1922; Inglaterra y la Palestina de 1939; el Holocausto; la independencia de Israel y la dura vida en 1949 y en 1952 en el flamante estado; la guerra fría y la de Corea de 1952; etcétera.

  3. Compositional variations of zirconolite from the Evate apatite deposit (Mozambique) as an indicator of magmatic-hydrothermal conditions during post-orogenic collapse of Gondwana

    Science.gov (United States)

    Hurai, Vratislav; Huraiová, Monika; Gajdošová, Michaela; Konečný, Patrik; Slobodník, Marek; Siegfried, Pete R.

    2018-06-01

    Zirconolite is documented from the Evate apatite-magnetite-carbonate deposit in the circular Monapo Klippe (eastern Mozambique)—a relic of Neoproterozoic nappe thrusted over the Mesoproterozoic basement of the Nampula block. Zirconolite enriched in rare earth elements—REE = Y + Lu+ΣLa-Yb (up to 24.11 wt% REE2O3, 0.596 apfu REE) creates thin rims around spinel and magnetite grains, whereas zirconolite enriched in U and Th (up to 18.88 wt% ThO2 + UO2, 0.293 apfu Th + U) replace the Late Ediacaran ( 590 Ma) zircon and baddeleyite along contacts with pyrrhotite and magnetite. Both types of zirconolite contain locally increased Nb and Ta concentrations (up to 7.58 wt% Nb2O5 + Ta2O5, 0.202 apfu Nb + Ta). Typical substitutions in zirconolite from Evate involve REE + U,Th → Ca, and M 2++ M 5+→Ti + M 3+ ( M 2+ = Fe2++Mg, M 3+ = Fe3+, M 5+ = Nb5++Ta5+). In addition, REE-zirconolite is typical of the REE + M 2+ → Ca + M 3+ substitution ( M 2+ = Mg, M 3+ = Fe3++Al3+). Hence, Fe3+ predominates over Fe2+ in all types of zirconolite, thus enabling the high REE content in Nb-poor zirconolites to be stored in locally dominant REEZrTiFe3+O7 component known so far only as a synthetic analogue of natural zirconolite. Other types of zirconolite from Evate are dominated by the common CaZrTi2O7 end member, but the aforementioned "synthetic" REEZrTiFe3+O7 accompanied by another `synthetic' (U,Th)ZrFe3 + 2O7 component are also abundant. The U,Pb,Th concentrations in U,Th-zirconolites plot discordantly to theoretical isochrons, thus indicating 440 ppm of non-radiogenic excess lead in earlier Nb-rich zirconolite contrasting with secondary Pb loss from later Nb-poor zirconolite. The non-radiogenic Pb-corrected age of the early zirconolite corresponded to 485 ± 9 Ma, within uncertainty limit identical with the 493 ± 10 Ma age of the associated uranothorianite. The variegated chemical composition of zirconolites reflects the complex history of the Evate deposit. Compositional and

  4. A report on 36 years practical work on crop improvement through induced mutagenesis

    Energy Technology Data Exchange (ETDEWEB)

    Datta, S.K. [CSIR, Madhyamgram Experimental Farm, Bose Institute, Kolkata (India)], E-mail: subodhskdatta@rediffmail.com

    2008-07-01

    Physical and/or chemical mutagens cause random changes in the nuclear DNA or cytoplasmic organelles, resulting in gene, chromosomal or genomic mutations. The author will share his life time experience and achievement on induced mutagenesis. The author initiated induced mutagenesis work in 1971 till July 2007 and used both physical (X-ray and Gamma rays) and chemical (EMS, MMS, Colchicine) mutagens for improvement of vegetables (Trichosanthes anguina L, T. cucumarina , Cucurbita maxima L, Cephalandra indica, Luffa acutangula Roxb., Lagenaria ciceraria), medicinal (Trigonella foenum-graecum L, Mentha citrate Ehrh), pulse (Winged Bean (Psophocarpus tetragonolobus L. D.C.), oil bearing (Jatropha curcas L, Rosa damascene, Cymbopogon flexuosus (Nees) Wats) and ornamental (Bougainvillea, Chrysanthemum, Dahlia, Gladiolus, Hibiscus, Lantana depressa Naud, Rose, Tuberose, Narcissus etc.) crops. All classical and advanced mutagenesis methods have been extensively used for the development of new and novel cultivars of economic importance. Early flowering, late flowering, dwarf, yellow fruit color, crinkled leaf, short thick fruit, increased branching, increased pod and seed number, seed size, seed color (green, brown, chocolate color) high fruit-, seed-, oil- and punicic acidyielding mutants have been developed in T. anguina, T. fornum-graecum, Winged Bean and in J.curcas containing 'curcas oil', an efficient substitute fuel for diesel engines. Induction of flower color and chlorophyll variegated mutants in L. depressa proved the efficiency of mutation technique for domestication of wild relatives. Author was deeply engaged for the last 30 years for improvement of ornamentals and has been most successful to produce quite a large number of new promising mutant varieties in different ornamentals. Colchicine has been successfully used to develop new flower color in chrysanthemum and rose and high yielding strains in T. anguina. A novel direct in vitro regeneration technique has

  5. [Plague in Zaire].

    Science.gov (United States)

    Janssens, P G; Pattyn, S R

    1994-01-01

    Two endemic foci of plague have been discovered in Zaïre, the first in the Ituri in 1928, the other in North-Kivu in 1938. They are situated in the region of the great East-African Rift and are adjacent to the Ugandan focus, identified in 1877. A strict surveillance of these endemic foci makes it possible to state that, between 1928 and 1959, 632 cases of plague have been diagnosed in the Ituri, or 20 a year, and 190 in the N-Kivu, or 8 a year. Since then several flare ups have been notified. This situation is very remote from the "black death" concept. Yersinia pestis presents, besides its bipolar staining, many other characteristics such as the indispensable presence of iron to produce virulence, or the fermentation of glycerine and reduction of nitrates as parameters for the identification of 3 biovars, corresponding with a specific geographic distribution: antiqua, medievalis, orientalis or maritima. The antigenic structure has been discussed and also the role of plasmids. Plague is a disease of rats, a variegated gathering of rodents with different degrees of tolerance and sensitiveness to Y.pestis, living in a frail equilibrium. The multimammate houserat was in the Ituri the principal agent until the black rat Rattus rattus invaded the region and a new balance came into being. The frequent changes in taxonomy of Mastomys caused uncertainties. The transmission is due to fleas subject to a blocking of their ventriculum by Y.pestis. Fleas play an active part in the process. Man is only a casual intruder. The pathogenicity is related to its invasiveness and its intracellular localization in macrophages and other R.E. cells, in which Y.pestis can survive. The bubo is characteristic of the disease. In Zaïre a septicaemic tendency has been observed, with a possible involvement of the C.N.S. and of the lungs. The latter may produce among the surrounding relatives primary pneumonic plague. The clinical diagnosis ought to be confirmed by bacteriologic investigation

  6. VOLUNTAD DE VENCER Y DE VENCERSE

    Directory of Open Access Journals (Sweden)

    Carla Cordua

    2005-11-01

    Full Text Available El ensayo enfoca a don Quijote desde el punto de vista del variado e intenso ejercicio de la voluntad que lo caracteriza; el protagonista de Cervantes decide por sí mismo cambiar de vida y darse una existencia nueva elegida libremente. Procede, sin vacilar, a preparar su salida al mundo como otro del que solía ser. Y, en efecto, su determinación voluntaria es tan poderosa que nada ni nadie lo separa de su determinación primera. El sentido de su brusca conversión es luchar contra diversos males y contra los agentes que lo practican impunemente. Decidido a vencer a los enemigos según el modelo caballeresco, busca también lograr la fama imperecedera del tipo que conquistan los andantes de sus novelas favoritas. La elección del modelo de existencia lo obliga a adoptar todas las reglas de vida y la moral caballeresca, sometiéndose así a su propia voluntad. Cabalgando a la ventura ha de velar sobre sí para no apartarse un ápice de los ideales que se ha autoimpuestoThe essay focuses on Don Quijote in the perspective of the intense and variegated exercise of the will that characterizes the knight-errant. Cervantes’ protagonist has decided for himself to change his life and give himself a new one freely chosen. Without hesitation, he makes ready to emerge in the world as other than he used to be. And, in fact, his voluntary determination is so strong that nothing nor any body can affect his free decision. The sense of his sudden conversión leads him to fight against different evils and their unpunished agents. His decision to overcome the enemies in accordance with the chivalry model, includes also the winning of the everlasting fame that comes to the wandering knights in his favorite novels. The election of this model of existence obliges him to adopt all the rules of living and morality proper to the chivalric code, overcoming thus his own will. Riding haphazard will be his sole protection to prevent the least slipping in the high ideals

  7. IDENTIFIKASI DINI GALUR KACANG TANAH YANG RESISTEN TERHADAP INFEKSI SCLEROTIUM ROLFSII HASIL SELEKSI IN VITRO DENGAN FILTRAT KULTUR S. ROLFSII

    Directory of Open Access Journals (Sweden)

    Yusnita, Widodo, Rita Megia, Hajrial Aswidinnoor dan Sudarsono .

    2012-02-01

    Full Text Available Early Identification of Sclerotium rolfsii Resistant Lines of Peanut Derived from In Vitro Selection Using S. rolfsii Culture Filtrates. The objectives of this experiment was to evaluate methods for early identification of Sclerotium rolfsii resistant lines derived from fungal culture filtrate (CF-insensitive somatic embryos (SE and to correlate the results of early identification with that of direct S. rolfsii inoculation in the plastic house.  In the first method, shoots regenerated from CF-insensitive SE were planted onto selective medium containing 30% of S. rolfsii CF for one month and then responses were evaluated. In the second method, leaves and leaflets of R0 peanut lines derived from CF-insensitive SE were inoculated with S. rolfsii by detached leaf-dual culture test. The damages of the inoculated leaves and leaflets were assessed 6 days after inoculation.  A number of R1 plants were grown in the plastic house from seeds harvested from R0 peanut lines, and they were inoculated with S. rolfsii at 30 days after planting (dap. Responses of the inoculated R1 plants were observed at 44 dap. Results of the experiment indicated that shoots from CF-insensitive SE showed less damage scores (DS=1.2, when cultured on CF-containing selective medium than ones from non-selected SE (DS=2.9. Most of the detached leaves and leaflets inoculated with S. rolfsii totally necrosed. However, leaves and leaflets from 7 out of 23 R0 lines showed variegation between necrosed and healthy tissues, indicating the presence of S. rolfsii resistant tissue among the tested leaves and leaflets. Direct inoculation of R1 plants in the plastic house showed 10 out of 18 progenies derived from 6 R0 lines showed less disease severity scores (DSS=2 or 3 and they were able to survive and produce seeds. On the other hand, seed derived (original peanut plants cv. Kelinci inoculated with S. rolfsii all showed DSS=5 and died after inoculation. The overall   results of the

  8. Correlations between potassium, rubidium and cesium ({sup 133}Cs and {sup 137}Cs) in sporocarps of Suillus variegatus in a Swedish boreal forest

    Energy Technology Data Exchange (ETDEWEB)

    Vinichuk, M., E-mail: Mykhailo.Vinichuk@slu.s [Department of Soil and Environment, Swedish University of Agricultural Sciences, P.O. Box 7014, SE-750 07 Uppsala (Sweden); Department of Ecology, Zhytomyr State Technological University, 103 Cherniakhovsky Str., 10005 Zhytomyr (Ukraine); Rosen, K.; Johanson, K.J. [Department of Soil and Environment, Swedish University of Agricultural Sciences, P.O. Box 7014, SE-750 07 Uppsala (Sweden); Dahlberg, A. [Department of Forest Mycology and Pathology, Swedish University of Agricultural Sciences, P.O. Box 7026, 750 07 Uppsala (Sweden)

    2011-04-15

    An analysis of sporocarps of ectomycorrhizal fungi Suillus variegatus assessed whether cesium ({sup 133}Cs and {sup 137}Cs) uptake was correlated with potassium (K) or rubidium (Rb) uptake. The question was whether intraspecific correlations of Rb, K and {sup 133}Cs mass concentrations with {sup 137}Cs activity concentrations in sporocarps were higher within, rather than among, different fungal species, and if genotypic origin of sporocarps within a population affected uptake and correlation. Sporocarps (n = 51) from a Swedish forest population affected by the fallout after the Chernobyl accident were studied. The concentrations were 31.9 {+-} 6.79 g kg{sup -1} for K (mean {+-} SD, dwt), 0.40 {+-} 0.09 g kg{sup -1} for Rb, 8.7 {+-} 4.36 mg kg{sup -1} for {sup 133}Cs and 63.7 {+-} 24.2 kBq kg{sup -1} for {sup 137}Cs. The mass concentrations of {sup 133}Cs correlated with {sup 137}Cs activity concentrations (r = 0.61). There was correlation between both {sup 133}Cs concentrations (r = 0.75) and {sup 137}Cs activity concentrations (r = 0.44) and Rb, but the {sup 137}Cs/{sup 133}Cs isotopic ratio negatively correlated with Rb concentration. Concentrations of K and Rb were weakly correlated (r = 0.51). The {sup 133}Cs mass concentrations, {sup 137}Cs activity concentrations and {sup 137}Cs/{sup 133}Cs isotopic ratios did not correlate with K concentrations. No differences between, within or, among genotypes in S. variegatus were found. This suggested the relationships between K, Rb, {sup 133}Cs and {sup 137}Cs in sporocarps of S. variegatus is similar to other fungal species. - Highlights: {yields} We studied uptake of Cs ({sup 133}Cs and {sup 137}Cs), K and Rb by Suillus variegates sporocarps. {yields} Genotypic origin of fungus did not affect uptake of studied elements (isotopes). {yields} Genotypic origin did not affect correlation between Cs ({sup 133}Cs and {sup 137}Cs), K and Rb.

  9. Etica, filosofia e mediazione linguistica: dall’Etica della filosofia occidentale al codice deontologico della mediazione linguistica

    Directory of Open Access Journals (Sweden)

    Mette Rudvin

    2016-01-01

    Full Text Available Abstract – Over the last few years, the issue of professional ethics has received much attention in the field of interpreting and translation, and in particular in the field of Community Interpreting (CI or Public Service Interpreting (PSI. (In this chapter we will refer to CI or PSI in Italian as ‘Mediazione Linguistica’. Today, ‘ethics’ figures prominently in the literature, in international conferences, in interpreting courses, in translation/interpreting mailing lists as well as in the working lives of professional interpreters. As an object of theoretical inquiry (meta-ethics as well as a guide for human conduct (normative ethics, ethics has been a prime focus of Western moral philosophy since the time of the Ancient Greeks. This chapter situates the main ethical tenets of the CI/PSI interpreting profession within the framework of the main principles of moral philosophy, namely notions of ‘good’, ‘virtue’, ‘duty’, ‘responsibility’, ‘utility’ and ‘consequence of actions’. The three principal ethical tenets of CI/PSI discussed in this chapter, Accuracy, Impartiality and Confidentiality, were identified on the basis of a general literature review and more specifically from a variegated (and to some degree representative sample of CI/PSI Codes of Ethics. The chapter argues that there is an underlying connection between the principal tenets of moral philosophy and those of the interpreting profession (which mirror similar ethical principles in other professions. The tenet of Accuracy could be seen as a ‘contract’ between interpreter and client, interpreter and source, interpreter and text/translation process/profession. The chapter situates the interpreter’s sense of duty and responsibility towards this tenet at an individual and collective level, within the Kantian tradition of Duty. The other two tenets – impartiality and confidentiality – safeguard the interpreter’s conduct towards the source

  10. Provenance analysis and thermo-dynamic studies of multi-type Holocene duricrusts (1700 BC) in the Sua Salt Pan, NE Botswana

    Science.gov (United States)

    Dill, Harald G.; Dohrmann, R.; Kaufhold, S.; Techmer, A.

    2014-08-01

    Multi-type duricrusts, composed of silcretes, calcretes, halcretes and sulcretes developed during the Holocene at the northern rim of the Sua Salt Pan, NE Botswana. They were investigated for their light (quartz/chalcedony, feldspar, analcime, clinoptilolite, calcite, kaolinite/halloysite, illite-smectite mixed-layers, halite) and heavy minerals (baryte, clinozoisite-epidote s.s.s., amphibole, corundum, tourmaline, ilmenite, rutile, sphene, kyanite, andalusite, staurolite, garnet, zircon, apatite, monazite, cassiterite, garnet, biotite) using petrographic microscopy, X-ray fluorescence and diffraction analyses, radio-carbon dating, scanning electron microscopy equipped with an EDX-system, cation exchange capacity and infrared spectroscopy. Detrital minerals predominantly derived from the erosion of rocks belonging to the Archaean Basement Complex, the Stormberg Volcanites and the Kalahari sediments. Of particular interest to exploration geologists, geikielite-enriched ilmenite fragments are a hint to kimberlitic pipes. Biodetritus was derived from invertebrates and from vertebrates (fish bones?). A man-made impact on the heavy mineral suite has to be invoked from small fragments of cassiterite fragments that derived from processing of sulfidic and pegmatitic Sn-bearing ore. In the salt-pan-derived duricrusts mainly the aeolian and to a lesser degree fluvial inputs were responsible for the concentration of clasts in these multi-type duricrusts. Moreover, their variegated mineralogy enables us to constrain the physical-chemical regime, prevalently as to the pH and the chemical composition of the major constituents. All duricrusts developed in a self-sufficient chemically closed system where quartz and feldspar provided the elements Si, Na, K, Ca, and Ba to produce the encrustations. The spatial and temporal trend in the Sua Salt Pan rim encrustations may be described as follows: (1) sulcrete-silcretes, (2) silcretes with kaolinite-group minerals towards more recent

  11. Radiation-induced mutation breeding of papaya

    Energy Technology Data Exchange (ETDEWEB)

    Chan, Y. K. [Horticulture Research Centre, Malaysian Agricultural Research and Development Institute (MARDI), Kuala Lumpur (Malaysia)

    2009-05-15

    Irradiation-induced mutation breeding of papaya commenced at the Malaysian Agricultural Research and Development Institute (MARDI) in August, 2000. This research was initiated under a Coordinated Research Project (CRP - D23023) with assistance from the International Atomic Energy Agency (IAEA). In the preliminary dosimetry study, seeds from two local papaya varieties, Sekaki and Eksotika were irradiated, either as dry seeds or as pre-soaked seeds (soaked overnight in water and surface-dried) with radiation doses ranging from 0 to 300 Gy. 100 Gy dose was lethal for all wet presoaked seeds while dry seed did not show loss of viability, even at 300 Gy. From the growth data it was estimated that dose of 525 Gy reduced shoot elongation by 50%, and this dose was recommended for mass irradiation of dry seeds. For wet, pre-soaked seeds results indicated that 42.5 Gy was the optimal dose for mass irradiation. At this dose, both seeds germination and seedlings growth were reduced by 50%. In a massive irradiation experiment 2,000 Eksotika seeds were irradiated at 42.5 Gy (pre-soaked) and another 2,000 at 525 Gy (dry). In the M2 population, numerous physiological defects were observed, including stem splitting, leaf variegation and puckering, and crinkled dwarfs. In the M3 population, a wide variability was recorded for a number of traits. M3 seedlings derived from presoaked seeds irradiated a low 42.5 Gy dose presented a high number of plants that were shorter and more vigorous in leaf development compared to those irradiated at 525 Gy and to non-irradiated control seedlings. The distribution patterns of M3 progenies for nine quantitative field characters showed great variation, often exceeding the limits of the control population. There appears to be good prospects in improving Eksotika papaya especially in the development of dwarf trees with lower fruit bearing stature, higher total soluble solids in fruits and larger fruit size. Several M2 and M3 putative mutants also

  12. Power for all? Electricity and uneven development in North Carolina

    Science.gov (United States)

    Harrison, Conor M.

    Many towns in eastern North Carolina face a number of challenges common to the rural South, including high rates of poverty and diminishing employment opportunities. However, some residents of this region also confront a unique hardship---electricity prices that are vastly higher than those of surrounding areas. This dissertation examines the origins of pricing inequalities in the electricity market of eastern North Carolina---namely how such inequalities developed and their role in the production of racial and economic disparities in the South. This dissertation examines the evolving relations between federal and state agencies, corporations, and electric utilities, and asks why these interactions produced varying social outcomes across different places and spatial settings. The research focuses on the origins and subsequent development of electric utilities in eastern North Carolina, and examines how electricity as a material technology interacted with geographies of race and class, as well as the dictates of capital accumulation. This approach enables a rethinking of several concepts that are rarely examined by scholars of electric utilities, most notably the monopoly service territory, which I argue served as a spatial fix to accumulation problems in the industry. Further, examining the way that electric utilities developed in North Carolina during the 20th century brings to the forefront the at times contradictory relationships among systems of electricity provision, Jim Crow segregation, the Progressive Era, and the New Deal. Such a focus highlights the important role that the control of electricity provision played in shaping racial inequalities that continue to persist in the region. With most urban areas were electrified in the 1930s, the research also traces the electricity distribution lines as they moved out of cities through rural electrification programs, a shift that highlights the state as a multi-scalar and variegated actor that both aided and

  13. Comparative genomic characterization of citrus-associated Xylella fastidiosa strains

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    Nunes Luiz R

    2007-12-01

    Full Text Available Abstract Background The xylem-inhabiting bacterium Xylella fastidiosa (Xf is the causal agent of Pierce's disease (PD in vineyards and citrus variegated chlorosis (CVC in orange trees. Both of these economically-devastating diseases are caused by distinct strains of this complex group of microorganisms, which has motivated researchers to conduct extensive genomic sequencing projects with Xf strains. This sequence information, along with other molecular tools, have been used to estimate the evolutionary history of the group and provide clues to understand the capacity of Xf to infect different hosts, causing a variety of symptoms. Nonetheless, although significant amounts of information have been generated from Xf strains, a large proportion of these efforts has concentrated on the study of North American strains, limiting our understanding about the genomic composition of South American strains – which is particularly important for CVC-associated strains. Results This paper describes the first genome-wide comparison among South American Xf strains, involving 6 distinct citrus-associated bacteria. Comparative analyses performed through a microarray-based approach allowed identification and characterization of large mobile genetic elements that seem to be exclusive to South American strains. Moreover, a large-scale sequencing effort, based on Suppressive Subtraction Hybridization (SSH, identified 290 new ORFs, distributed in 135 Groups of Orthologous Elements, throughout the genomes of these bacteria. Conclusion Results from microarray-based comparisons provide further evidence concerning activity of horizontally transferred elements, reinforcing their importance as major mediators in the evolution of Xf. Moreover, the microarray-based genomic profiles showed similarity between Xf strains 9a5c and Fb7, which is unexpected, given the geographical and chronological differences associated with the isolation of these microorganisms. The newly

  14. What is the Difference in Morphologic Features of the Thoracic Pedicle Between Patients With Adolescent Idiopathic Scoliosis and Healthy Subjects? A CT-based Case-control Study.

    Science.gov (United States)

    Gao, Bo; Gao, Wenjie; Chen, Chong; Wang, Qinghua; Lin, Shaochun; Xu, Caixia; Huang, Dongsheng; Su, Peiqiang

    2017-11-01

    the most variegated region of the curve with the highest prevalence of dysplastic pedicles. Our study can help surgeons perform preoperative assessments in females with adolescent idiopathic scoliosis, and with preoperative and intraoperative management for difficult pedicle screw placement. In particular, our results suggest that surgeons should exercise increased vigilance when selecting pedicle screw dimensions, especially in the concave aspect of the mid-thoracic curve, to avoid cortical breeches. Future studies should evaluate other Lenke types of adolescent idiopathic scoliosis, and males with adolescent idiopathic scoliosis.

  15. Bioassay assessment of metarhizium anisopliae (metchnikoff sorokin (deuteromycota: hyphomycetes against Oncometopia facialis (signoret (hemiptera: cicadellidae Avaliação do bioensaio de Metarhizium anisopliae (metchnikoff sorokin (deuteromycota: hyphomycetes contra Oncometopia facialis (signoret (hemiptera: cicadellidae

    Directory of Open Access Journals (Sweden)

    Wolney Dalla Pria Júnior

    2008-03-01

    Full Text Available Citrus Variegated Chlorosis (CVC is an economically important, destructive disease in Brazil and is caused by Xylella fastidiosa and transmitted by sharpshooter insects. In this study, the efficacy of the fungus Metarhizium anisopliae in controlling the sharpshooter Oncometopia facialis was studied by bioassay conditions. In the bioassay, insects were sprayed with a suspension containing 5 X 10(7 conidia mL-1. Adults captured in the field were treated in groups of 10 in a total of 11 replications per treatment. Significant differences between the natural mortality and the mortality of insects treated with the fungus were observed 6 days after inoculations (PA Clorose Variegada dos Citros (CVC é uma doença economicamente importante e destrutiva no Brasil e é causada pela bactéria Xylella fastidiosa e transmitida por insetos vetores tal como Oncometopia facialis. Nesse estudo, a eficácia do fungo Metarhizium anisopliae em controlar o inseto vetor O. facialis foi estudada em condições de bioensaio. Nesse bioensaio, insetos foram pulverizados com uma suspensão de 5 X 10(7 conídio mL-1. Insetos-adultos capturados no campo foram tratados em grupos de 10, em um total de 11 replicatas por tratamento. Diferenças significativas entre a mortalidade natural e a mortalidade dos insetos tratados com o fungo foram observadas em 6 dias após a inoculação (P<0.05. Estas diferenças significativas aumentaram antes do décimo dia após o tratamento. O fungo causou uma mortalidade de 87,1%, com uma LT50 variando entre 5 e 6 dias. A LC50 foi de 1,2 X 10(6 conídio mL-1, variando de 7,7 X 10(5 a 2 X 10(6 conídio mL-1. Estes resultados mostraram que o vetor O. facialis foi susceptível a ação entomopatogênica de M. anisopliae em condições controladas durante o bioensaio.

  16. Rooting of healthy and CVC-affected 'Valência' sweet orange stem cuttings, through the use of plant regulators

    Directory of Open Access Journals (Sweden)

    Gustavo Habermann

    2006-01-01

    Full Text Available Citrus variegated chlorosis (CVC is a disease caused by Xylella fastidiosa. Using different concentrations of plant regulators, such as auxins (indole-3-butyric acid and gibberellic acid biosynthesis-inhibitor (paclobutrazol, physiological rooting capacity of healthy and CVC-affected stem cuttings were evaluated in order to investigate the importance of plant hormone imbalance and xylem occlusion in plants with CVC. The percentages of dead, alive and rooted cuttings, cuttings with callus and mean number of roots per cuttings did not show statistical differences in response to the distinct concentrations of synthetic plant regulators. There were differences only between healthy and CVC-affected cuttings. This showed the importance of xylem occlusion and diffusive disturbances in diseased plants, in relation to root initiation capacity and hormonal translocation in the plant tissue.Clorose variegada dos citros (CVC é uma doença causada por Xylella fastidiosa, podendo determinar oclusão do xilema e desbalanço hormonal, o que por fim está relacionado ao processo de iniciação radicial em estacas. Usando diferentes concentrações de fitorreguladores, como auxinas (ácido 3-indol butírico e inibidores da biossíntese de ácido giberélico (paclobutrazol, que são promotores do enraizamento de estacas, verificou-se a capacidade fisiológica de enraizamento de estacas sadias e com CVC, a fim de investigar a importância do desbalanço hormonal e oclusão do xilema em plantas doentes. As porcentagens de estacas mortas, vivas, enraizadas e com calo e o número médio de raízes por estaca não mostraram diferenças estatísticas em resposta às diferentes concentrações dos reguladores vegetais sintéticos. Houve diferenças apenas entre estacas sadias e doentes. Isto aponta a importância da oclusão do xilema e distúrbios difusivos em plantas doentes, em relação à capacidade de iniciação radicial e à translocação hormonal no tecido

  17. Heterochromatin protein 1 (HP1a positively regulates euchromatic gene expression through RNA transcript association and interaction with hnRNPs in Drosophila.

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    Lucia Piacentini

    2009-10-01

    Full Text Available Heterochromatin Protein 1 (HP1a is a well-known conserved protein involved in heterochromatin formation and gene silencing in different species including humans. A general model has been proposed for heterochromatin formation and epigenetic gene silencing in different species that implies an essential role for HP1a. According to the model, histone methyltransferase enzymes (HMTases methylate the histone H3 at lysine 9 (H3K9me, creating selective binding sites for itself and the chromodomain of HP1a. This complex is thought to form a higher order chromatin state that represses gene activity. It has also been found that HP1a plays a role in telomere capping. Surprisingly, recent studies have shown that HP1a is present at many euchromatic sites along polytene chromosomes of Drosophila melanogaster, including the developmental and heat-shock-induced puffs, and that this protein can be removed from these sites by in vivo RNase treatment, thus suggesting an association of HP1a with the transcripts of many active genes. To test this suggestion, we performed an extensive screening by RIP-chip assay (RNA-immunoprecipitation on microarrays, and we found that HP1a is associated with transcripts of more than one hundred euchromatic genes. An expression analysis in HP1a mutants shows that HP1a is required for positive regulation of these genes. Cytogenetic and molecular assays show that HP1a also interacts with the well known proteins DDP1, HRB87F, and PEP, which belong to different classes of heterogeneous nuclear ribonucleoproteins (hnRNPs involved in RNA processing. Surprisingly, we found that all these hnRNP proteins also bind heterochromatin and are dominant suppressors of position effect variegation. Together, our data show novel and unexpected functions for HP1a and hnRNPs proteins. All these proteins are in fact involved both in RNA transcript processing and in heterochromatin formation. This suggests that, in general, similar epigenetic mechanisms

  18. Genetic characterization of Xylella fastidiosa isolated from citrus and coffee plants Caracterização genética de Xylella fastidiosa isolada de plantas de citros e café

    Directory of Open Access Journals (Sweden)

    Vicente Savonitti Miranda

    2007-10-01

    Full Text Available The Citrus Variegated Chlorosis and the Coffee Leaf Scorch are some of the many destructive diseases caused by Xylella fastidiosa, a gram-negative bacterium limited to the xylem of affected plants. As its genetic characterization is still not well established, different isolates of X. fastidiosa from citrus and coffee were evaluated through RAPD (Random Amplified Polymorphic DNA technique to characterize and classify these isolates based on similarity coefficients. Sixteen isolates of X. fastidiosa were used on this trial, obtained from citrus, coffee and almond. The genetic polymorphism evaluation was performed using six arbitrary 10-base primer pairs. It was possible to establish a dendogram in which the isolates were classified into five groups (A, B, C, D and E. A prevalence of citrus isolates in groups A and D was observed. In groups B and C, there was a prevalence of coffee isolates meanwhile the group D consisted of the almond isolate, solely.A Clorose Variegada dos Citros e a Requeima das Folhas do Cafeeiro são algumas das várias doenças destrutivas causadas pela Xylella fastidiosa, que é uma bactéria gram-negativa e limitada ao xilema de plantas afetadas. Como a sua caracterização genética ainda não está determinada, diferentes isolados da X. fastidiosa de citros e café foram avaliados pela técnica RAPD (Polimorfismo do DNA Amplificado ao Acaso para caracterizar e classificar estes isolados com base em coeficientes de similaridade. Foram utilizados 16 isolados de X. fastidiosa provenientes de citros, café e amêndoa. A avaliação do polimorfismo genético foi realizada utilizando seis iniciadores randômicos de 10 pares de base. Foi possível estabelecer um dendograma no qual os isolados foram classificados em cinco grupos (A, B, C, D e E. Nos grupos A e D existe uma forte predominância de isolados de citros. Nos grupos B e C há predominância de isolados de café enquanto no grupo E ficou apenas o isolado de amêndoa.

  19. Regional hardening of Upper Cretaceous Chalk in eastern England, UK: trace element and stable isotope patterns in the Upper Cenomanian and Turonian Chalk and their significance

    Science.gov (United States)

    Jeans, Christopher V.; Long, Dee; Hu, Xiu-Fang; Mortimore, Rory N.

    2014-12-01

    The regional hardening of the Late Cenomanian to Early Turonian Chalk of the Northern Province of eastern England has been investigated by examining the pattern of trace elements and stable carbon and oxygen isotopes in the bulk calcite of two extensive and stratigraphically adjacent units each 4 to 5 m thick of hard chalk in Lincolnshire and Yorkshire. These units are separated by a sequence, 0.3-1.3 m thick, of variegated marls and clayey marls. Modelling of the geochemistry of the hard chalk by comparison with the Standard Louth Chalk, combined with associated petrographic and geological evidence, indicates that (1) the hardening is due to the precipitation of a calcite cement, and (2) the regional and stratigraphical patterns of geochemical variation in the cement are largely independent of each other and have been maintained by the impermeable nature of the thin sequence of the clay-rich marls that separate them. Two phases of calcite cementation are recognised. The first phase was microbially influenced and did not lithify the chalk. It took place predominantly in oxic and suboxic conditions under considerable overpressure in which the Chalk pore fluids circulated within the units, driven by variations in compaction, temperature, pore fluid pressure and local tectonics. There is evidence in central and southern Lincolnshire of the loss of Sr and Mgenriched pore fluids to the south during an early part of this phase. The second phase of calcite precipitation was associated with the loss of overpressure in probably Late Cretaceous and in Cenozoic times as the result of fault movement in the basement penetrating the overlying Chalk and damaging the seal between the two chalk units. This greatly enhanced grain pressures, resulting in grain welding and pressure dissolution, causing lithification with the development of stylolites, marl seams, and brittle fractures. Associated with this loss of overpressure was the penetration of the chalk units by allochthonous

  20. The arms race between heliconiine butterflies and Passiflora plants - new insights on an ancient subject.

    Science.gov (United States)

    de Castro, Érika C P; Zagrobelny, Mika; Cardoso, Márcio Z; Bak, Søren

    2018-02-01

    Heliconiines are called passion vine butterflies because they feed exclusively on Passiflora plants during the larval stage. Many features of Passiflora and heliconiines indicate that they have radiated and speciated in association with each other, and therefore this model system was one of the first examples used to exemplify coevolution theory. Three major adaptations of Passiflora plants supported arguments in favour of their coevolution with heliconiines: unusual variation of leaf shape within the genus; the occurrence of yellow structures mimicking heliconiine eggs; and their extensive diversity of defence compounds called cyanogenic glucosides. However, the protection systems of Passiflora plants go beyond these three features. Trichomes, mimicry of pathogen infection through variegation, and production of extrafloral nectar to attract ants and other predators of their herbivores, are morphological defences reported in this plant genus. Moreover, Passiflora plants are well protected chemically, not only by cyanogenic glucosides, but also by other compounds such as alkaloids, flavonoids, saponins, tannins and phenolics. Heliconiines can synthesize cyanogenic glucosides themselves, and their ability to handle these compounds was probably one of the most crucial adaptations that allowed the ancestor of these butterflies to feed on Passiflora plants. Indeed, it has been shown that Heliconius larvae can sequester cyanogenic glucosides and alkaloids from their host plants and utilize them for their own benefit. Recently, it was discovered that Heliconius adults have highly accurate visual and chemosensory systems, and the expansion of brain structures that can process such information allows them to memorize shapes and display elaborate pre-oviposition behaviour in order to defeat visual barriers evolved by Passiflora species. Even though the heliconiine-Passiflora model system has been intensively studied, the forces driving host-plant preference in these

  1. The CNS in inbred transgenic models of 4-repeat Tauopathy develops consistent tau seeding capacity yet focal and diverse patterns of protein deposition.

    Science.gov (United States)

    Eskandari-Sedighi, Ghazaleh; Daude, Nathalie; Gapeshina, Hristina; Sanders, David W; Kamali-Jamil, Razieh; Yang, Jing; Shi, Beipei; Wille, Holger; Ghetti, Bernardino; Diamond, Marc I; Janus, Christopher; Westaway, David

    2017-10-04

    MAPT mutations cause neurodegenerative diseases such as frontotemporal dementia but, strikingly, patients with the same mutation may have different clinical phenotypes. Given heterogeneities observed in a transgenic (Tg) mouse line expressing low levels of human (2 N, 4R) P301L Tau, we backcrossed founder stocks of mice to C57BL/6Tac, 129/SvEvTac and FVB/NJ inbred backgrounds to discern the role of genetic versus environmental effects on disease-related phenotypes. Three inbred derivatives of a TgTau P301L founder line had similar quality and steady-state quantity of Tau production, accumulation of abnormally phosphorylated 64-68 kDa Tau species from 90 days of age onwards and neuronal loss in aged Tg mice. Variegation was not seen in the pattern of transgene expression and seeding properties in a fluorescence-based cellular assay indicated a single "strain" of misfolded Tau. However, in other regards, the aged Tg mice were heterogeneous; there was incomplete penetrance for Tau deposition despite maintained transgene expression in aged animals and, for animals with Tau deposits, distinctions were noted even within each subline. Three classes of rostral deposition in the cortex, hippocampus and striatum accounted for 75% of pathology-positive mice yet the mean ages of mice scored as class I, II or III were not significantly different and, hence, did not fit with a predictable progression from one class to another defined by chronological age. Two other patterns of Tau deposition designated as classes IV and V, occurred in caudal structures. Other pathology-positive Tg mice of similar age not falling within classes I-V presented with focal accumulations in additional caudal neuroanatomical areas including the locus coeruleus. Electron microscopy revealed that brains of Classes I, II and IV animals all exhibit straight filaments, but with coiled filaments and occasional twisted filaments apparent in Class I. Most strikingly, Class I, II and IV animals presented

  2. MRI findings of intracranial cavernous malformations

    International Nuclear Information System (INIS)

    Han, Byoung Hee; Kim, Dong Ik; Cho, Yong Kuk

    1995-01-01

    To analyze the variable MRI features and clinical significance of intracranial cavernous malformations. Forty patients(mean age 35.4) with cavernous malformation were evaluated by MRI. Eleven patients were surgically confirmed. Cavernous malformations were divided into four categories on the basis of the MR imaging characteristics, especially on T2-weighted image. Type I lesion was defined as an extralesional subacute hemorrhage outside the low signal rim, type II as an intralesional hemorrhage surrounded by low signal rim, type III lesion as an intralesional thrombosis with variegated central core surrounded by low signal rim, and type IV lesion as a focal old hemorrhagic core with small low signal intensity. Type IV was further divided into IVa and IVb, whether the lesion has small iso-or hypersignal central core (IVa) or not (IVb). Follow-up MRI was evaluated in 12 patients who were managed conservatively. Follow-up intervals ranged from 2 weeks to 29 months (mean 6 months). Total 80 lesions were detected in 40 patients. Multiple lesions were noted in 10 patients. The topography of the cavernous malformations was supratentorial in 75% and infratentorial in 23%. There were 10 lesions in type I, 15 in type II, 21 in type III, 14 in type IVa, and 20 in type IVb. Type I lesions mainly showed mass effect and edema. Type III lesions showed minimal contrast enhancements in 7 lesions on delayed images. Type II lesions showed the characteristics of both type I and type III lesions. On follow up images, decrease in size in 5, change of type in 7, rebleeding in 2 and no change in 12 lesions were demonstrated. Hemorrhage, edema and mass effect were combined in the cases of rebleeding. On follow-up study, the estimated risk of bleeding was 32.3%/person-year and 13.7%/lesion-year. Cavernous malformations show as variable appearance, on MR imaging suggesting variable stages of evolution. The MR morphologic classification and evaluation of secondary findings are helpful to

  3. La Llegenda del llibreter assassí de Barcelona. Tradizione e traduzione. Note al margine dell’edizione italiana

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    Inés Ravasini

    2013-12-01

    Full Text Available La recente pubblicazione in Italia della traduzione de La llegenda del llibreter assassí di Ramon Miquel i Planas offre lo spunto per un confronto con alcune precedenti traduzioni in castigliano e per una breve riflessione di metodo sulla traduzione. Le due versioni spagnole (1991 e 2011 si configurano infatti, a differenza di quella italiana, come libere rielaborazioni dell’originale dal momento che ne alterano la struttura, trasformando il testo e piegandolo a nuove finalità. L’elemento di interesse di questa «manipolazione» risiede nel fatto che essa è in qualche modo autorizzata dalla stessa natura miscellanea dell’opera di Miquel i Planas, al contempo libro erudito e antologia di materiali narrativi diversi, e dalle variegate forme di trasmissione dei materiali leggendari raccolti dallo studioso catalano. Le versioni della Llegenda consentono così d’iscrivere la riflessione sul tradurre nell’ambito della ricezione e dell’influsso esercitato dalla tradizione di un testo sulle sue modalità di traduzione. The recent publication of the Italian translation of La llegenda del llibreter assassí by Ramon Miquel i Planas provides an opportunity to make a comparison with some previous Spanish translations and to reflect briefly on translation methods. The two Spanish versions (1991 and 2011, unlike the Italian one, are free reworkings of the original, in the sense that they alter the structure, transform the text and shape it to new ends. What is interesting about this «manipulation» is that it is in some way legitimised by the heterogeneous nature of Miquel i Planas’s work, which is both an erudite volume and an anthology of different narrative materials, as well as by the varied ways in which the Catalan scholar handles materials relating to legends. The versions of Llegenda thus permit the inclusion of this reflection on translation in the sphere of the reception of a text and of the influence exerted by the tradition

  4. Web Server Embedded System

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    Adharul Muttaqin

    2014-07-01

    Full Text Available Abstrak Embedded sistem saat ini menjadi perhatian khusus pada teknologi komputer, beberapa sistem operasi linux dan web server yang beraneka ragam juga sudah dipersiapkan untuk mendukung sistem embedded, salah satu aplikasi yang dapat digunakan dalam operasi pada sistem embedded adalah web server. Pemilihan web server pada lingkungan embedded saat ini masih jarang dilakukan, oleh karena itu penelitian ini dilakukan dengan menitik beratkan pada dua buah aplikasi web server yang tergolong memiliki fitur utama yang menawarkan “keringanan” pada konsumsi CPU maupun memori seperti Light HTTPD dan Tiny HTTPD. Dengan menggunakan parameter thread (users, ramp-up periods, dan loop count pada stress test embedded system, penelitian ini menawarkan solusi web server manakah diantara Light HTTPD dan Tiny HTTPD yang memiliki kecocokan fitur dalam penggunaan embedded sistem menggunakan beagleboard ditinjau dari konsumsi CPU dan memori. Hasil penelitian menunjukkan bahwa dalam hal konsumsi CPU pada beagleboard embedded system lebih disarankan penggunaan Light HTTPD dibandingkan dengan tiny HTTPD dikarenakan terdapat perbedaan CPU load yang sangat signifikan antar kedua layanan web tersebut Kata kunci: embedded system, web server Abstract Embedded systems are currently of particular concern in computer technology, some of the linux operating system and web server variegated also prepared to support the embedded system, one of the applications that can be used in embedded systems are operating on the web server. Selection of embedded web server on the environment is still rarely done, therefore this study was conducted with a focus on two web application servers belonging to the main features that offer a "lightness" to the CPU and memory consumption as Light HTTPD and Tiny HTTPD. By using the parameters of the thread (users, ramp-up periods, and loop count on a stress test embedded systems, this study offers a solution of web server which between the Light

  5. Mathematics make microbes beautiful, beneficial, and bountiful.

    Science.gov (United States)

    Jungck, John R

    2012-01-01

    Microbiology is a rich area for visualizing the importance of mathematics in terms of designing experiments, data mining, testing hypotheses, and visualizing relationships. Historically, Nobel Prizes have acknowledged the close interplay between mathematics and microbiology in such examples as the fluctuation test and mutation rates using Poisson statistics by Luria and Delbrück and the use of graph theory of polyhedra by Caspar and Klug. More and more contemporary microbiology journals feature mathematical models, computational algorithms and heuristics, and multidimensional visualizations. While revolutions in research have driven these initiatives, a commensurate effort needs to be made to incorporate much more mathematics into the professional preparation of microbiologists. In order not to be daunting to many educators, a Bloom-like "Taxonomy of Quantitative Reasoning" is shared with explicit examples of microbiological activities for engaging students in (a) counting, measuring, calculating using image analysis of bacterial colonies and viral infections on variegated leaves, measurement of fractal dimensions of beautiful colony morphologies, and counting vertices, edges, and faces on viral capsids and using graph theory to understand self assembly; (b) graphing, mapping, ordering by applying linear, exponential, and logistic growth models of public health and sanitation problems, revisiting Snow's epidemiological map of cholera with computational geometry, and using interval graphs to do complementation mapping, deletion mapping, food webs, and microarray heatmaps; (c) problem solving by doing gene mapping and experimental design, and applying Boolean algebra to gene regulation of operons; (d) analysis of the "Bacterial Bonanza" of microbial sequence and genomic data using bioinformatics and phylogenetics; (e) hypothesis testing-again with phylogenetic trees and use of Poisson statistics and the Luria-Delbrück fluctuation test; and (f) modeling of

  6. Beyond "Monologicality"? Exploring Conspiracist Worldviews.

    Science.gov (United States)

    Franks, Bradley; Bangerter, Adrian; Bauer, Martin W; Hall, Matthew; Noort, Mark C

    2017-01-01

    journey of conversion, variegated kinds of political action, and optimistic belief in future change. These findings are at odds with the typical image of monological CT believers as paranoid, cynical, anomic and irrational. For many, the CT worldview may rather constitute the ideological underpinning of a nascent pre-figurative social movement.

  7. Beyond “Monologicality”? Exploring Conspiracist Worldviews

    Directory of Open Access Journals (Sweden)

    Bradley Franks

    2017-06-01

    representation of the outgroup, a personal journey of conversion, variegated kinds of political action, and optimistic belief in future change. These findings are at odds with the typical image of monological CT believers as paranoid, cynical, anomic and irrational. For many, the CT worldview may rather constitute the ideological underpinning of a nascent pre-figurative social movement.

  8. Beyond “Monologicality”? Exploring Conspiracist Worldviews

    Science.gov (United States)

    Franks, Bradley; Bangerter, Adrian; Bauer, Martin W.; Hall, Matthew; Noort, Mark C.

    2017-01-01

    outgroup, a personal journey of conversion, variegated kinds of political action, and optimistic belief in future change. These findings are at odds with the typical image of monological CT believers as paranoid, cynical, anomic and irrational. For many, the CT worldview may rather constitute the ideological underpinning of a nascent pre-figurative social movement. PMID:28676768

  9. Genetics and fine mapping of a purple leaf gene, BoPr, in ornamental kale (Brassica oleracea L. var. acephala).

    Science.gov (United States)

    Liu, Xiao-Ping; Gao, Bao-Zhen; Han, Feng-Qing; Fang, Zhi-Yuan; Yang, Li-Mei; Zhuang, Mu; Lv, Hong-Hao; Liu, Yu-Mei; Li, Zhan-Sheng; Cai, Cheng-Cheng; Yu, Hai-Long; Li, Zhi-Yuan; Zhang, Yang-Yong

    2017-03-14

    Due to its variegated and colorful leaves, ornamental kale (Brassica oleracea L. var. acephala) has become a popular ornamental plant. In this study, we report the fine mapping and analysis of a candidate purple leaf gene using a backcross population and an F 2 population derived from two parental lines: W1827 (with white leaves) and P1835 (with purple leaves). Genetic analysis indicated that the purple leaf trait is controlled by a single dominant gene, which we named BoPr. Using markers developed based on the reference genome '02-12', the BoPr gene was preliminarily mapped to a 280-kb interval of chromosome C09, with flanking markers M17 and BoID4714 at genetic distances of 4.3 cM and 1.5 cM, respectively. The recombination rate within this interval is almost 12 times higher than the usual level, which could be caused by assembly error for reference genome '02-12' at this interval. Primers were designed based on 'TO1000', another B. oleracea reference genome. Among the newly designed InDel markers, BRID485 and BRID490 were found to be the closest to BoPr, flanking the gene at genetic distances of 0.1 cM and 0.2 cM, respectively; the interval between the two markers is 44.8 kb (reference genome 'TO1000'). Seven annotated genes are located within the 44.8 kb genomic region, of which only Bo9g058630 shows high homology to AT5G42800 (dihydroflavonol reductase), which was identified as a candidate gene for BoPr. Blast analysis revealed that this 44.8 kb interval is located on an unanchored scaffold (Scaffold000035_P2) of '02-12', confirming the existence of assembly error at the interval between M17 and BoID4714 for reference genome '02-12'. This study identified a candidate gene for BoPr and lays a foundation for the cloning and functional analysis of this gene.

  10. DURABILTY OF 25 LOCAL SPECIFIC WOOD SPECIES FROM JAVA PRESERVED WITH CCB AGAINST MARINE BORERS ATTACK

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    Mohammad Muslich

    2010-12-01

    Full Text Available This study was conducted to provide basis information of the 25 local specific wood species indigenous from Java treated by copper bichromated boron (CCB. The full-cell process for 2 hours and 150 psi during the pressure-keeping period was employed. The IUFRO method was applied for the determination of wood treatability class. The treated and untreated wood specimens were tied together using plastic cord, arranged into a raft like assembly, and then exposed for 3, 6, and 12 months to the brackish water situated at Rambut Island’s coastal area. The Nordic Wood Preservation Council (NWPC standard No.1.4.2.2/75 was used to determine the intensity of marine borer infestation. The results revealed that 19 out of those 25 species were classified as easy to be preser ved, four species as moderate, and the remaining two were difficult to be preser ved. Those 19 species, i.e. Tamarindus indica L., Diplodiscus sp., Ficus variegate R .Br., Ehretia acuminata R .Br., Meliocope lunu-ankenda (Gaertn T.G. Hartley, Colona javanica B.L., Pouteria duclitanBachni., Stercularia oblongata R .Br., Ficus vasculosa Wall ex Miq., Callophyllum grandiflorum JJS., Turpinia sphaerocarpa Hassk., Neolitsea triplinervia Merr., Acer niveum Bl., Sloanea sigun Szysz., Castanopsis acuminatissima A.DC., Cinnamomum iners Reinw. Ex Blume., Litsea angulata Bl., Ficus nervosa Heyne., and Horsfieldia glabra Warb. were more permeable implying that the CCB retention and penetration were greater and deeper. Hymeneaecarboril.L., LitseaodoriferaVal., Gironniera subasqualisPlanch., and LinderapolyanthaBoerl. were moderately permeable. Castanopsis tunggurut A.DC. and Azadirachta indica Juss. were the least permeable judging that the CCB retention and penetration were lowest and shallowest. The treated wood specimens in this regard were able to prevent marine borers attack. Meanwhile, the untreated specimens were susceptible to marine borers attack, except Azadirachta indica. The attacking

  11. About soil cover heterogeneity of agricultural research stations' experimental fields

    Science.gov (United States)

    Rannik, Kaire; Kõlli, Raimo; Kukk, Liia

    2013-04-01

    , which landscape is characterized by till and limestone plains with thin Quaternary cover, the soil cover is more heterogeneous than in previous area. Kuusiku soil cover is more variegated by the soil texture and as well as by the genesis of soils. In addition to Cambisols, Leptosols, Gleysols and Luvisols may be found here as well. The dominating soils in Olustvere research area, which is situated on wavy upland plateau, are Albeluvisols.

  12. Drug discovery strategies in the field of tumor energy metabolism: Limitations by metabolic flexibility and metabolic resistance to chemotherapy.

    Science.gov (United States)

    Amoedo, N D; Obre, E; Rossignol, R

    2017-08-01

    The search for new drugs capable of blocking the metabolic vulnerabilities of human tumors has now entered the clinical evaluation stage, but several projects already failed in phase I or phase II. In particular, very promising in vitro studies could not be translated in vivo at preclinical stage and beyond. This was the case for most glycolysis inhibitors that demonstrated systemic toxicity. A more recent example is the inhibition of glutamine catabolism in lung adenocarcinoma that failed in vivo despite a strong addiction of several cancer cell lines to glutamine in vitro. Such contradictory findings raised several questions concerning the optimization of drug discovery strategies in the field of cancer metabolism. For instance, the cell culture models in 2D or 3D might already show strong limitations to mimic the tumor micro- and macro-environment. The microenvironment of tumors is composed of cancer cells of variegated metabolic profiles, supporting local metabolic exchanges and symbiosis, but also of immune cells and stroma that further interact with and reshape cancer cell metabolism. The macroenvironment includes the different tissues of the organism, capable of exchanging signals and fueling the tumor 'a distance'. Moreover, most metabolic targets were identified from their increased expression in tumor transcriptomic studies, or from targeted analyses looking at the metabolic impact of particular oncogenes or tumor suppressors on selected metabolic pathways. Still, very few targets were identified from in vivo analyses of tumor metabolism in patients because such studies are difficult and adequate imaging methods are only currently being developed for that purpose. For instance, perfusion of patients with [ 13 C]-glucose allows deciphering the metabolomics of tumors and opens a new area in the search for effective targets. Metabolic imaging with positron emission tomography and other techniques that do not involve [ 13 C] can also be used to evaluate tumor

  13. Diagenetic Iron Cycling in Ancient Alkaline Saline Lacustrine Sedimentary Rocks: A Case Study on the Jurassic Brushy Basin Member of the Morrison Formation, Colorado Plateau, USA

    Science.gov (United States)

    Potter-McIntyre, S. L.; Chan, M. A.; McPherson, B. J. O. L.

    2014-12-01

    The upper part of the Brushy Basin Member in the Four Corners region of the U.S. was deposited in an ephemeral alkaline saline lake system with copious input of volcanic ash. The variegated shale formation provides a setting for the study of early diagenetic iron cycling that records the action of alkaline saline fluid chemistries reacting with volcaniclastic sediments in the presence of microbes. A bull's-eye pattern of authigenic minerals with increasing alteration towards the basinal center similar to modern alkaline saline lakes provides evidence for an extreme paleoenvironmental interpretation. The purpose of this research is to document specific factors, such as reactive sediments, microbial influences, and grain size that affect concretion formation and iron cycling in an ancient extreme environment. Three broad diagenetic facies are interpreted by color and associated bioturbation features: red, green and intermediate. Diagenetic facies reflect meter-scale paleotopography: red facies represent shallow water to subaerial, oxidizing conditions; green facies reflect saturated conditions and reducing pore water chemistry shortly after deposition, and intermediate facies represent a combination of the previous two conditions. Evidence of biotic influence is abundant and trace fossils exhibit patterns associated with the diagenetic facies. Red diagenetic facies typically contain burrows and root traces and green diagenetic facies exhibit restricted biotic diversity typically limited to algal molds (vugs). Microbial fossils are well-preserved and are in close proximity to specific iron mineral textures suggesting biotic influence on the crystal morphology. Three categories of concretions are characterized based on mineralogy: carbonate, iron (oxyhydr)oxide and phosphate concretions. Concretion mineralogy and size vary within an outcrop and even within a stratigraphic horizon such that more than one main category is typically present in an outcrop. Variation in

  14. Compositional variations of zirconolite from the Evate apatite deposit (Mozambique) as an indicator of magmatic-hydrothermal conditions during post-orogenic collapse of Gondwana

    Science.gov (United States)

    Hurai, Vratislav; Huraiová, Monika; Gajdošová, Michaela; Konečný, Patrik; Slobodník, Marek; Siegfried, Pete R.

    2017-11-01

    Zirconolite is documented from the Evate apatite-magnetite-carbonate deposit in the circular Monapo Klippe (eastern Mozambique)—a relic of Neoproterozoic nappe thrusted over the Mesoproterozoic basement of the Nampula block. Zirconolite enriched in rare earth elements—REE = Y + Lu+ΣLa-Yb (up to 24.11 wt% REE2O3, 0.596 apfu REE) creates thin rims around spinel and magnetite grains, whereas zirconolite enriched in U and Th (up to 18.88 wt% ThO2 + UO2, 0.293 apfu Th + U) replace the Late Ediacaran ( 590 Ma) zircon and baddeleyite along contacts with pyrrhotite and magnetite. Both types of zirconolite contain locally increased Nb and Ta concentrations (up to 7.58 wt% Nb2O5 + Ta2O5, 0.202 apfu Nb + Ta). Typical substitutions in zirconolite from Evate involve REE + U,Th → Ca, and M 2++M 5+→Ti + M 3+ (M 2+ = Fe2++Mg, M 3+ = Fe3+, M 5+ = Nb5++Ta5+). In addition, REE-zirconolite is typical of the REE + M 2+ → Ca + M 3+ substitution (M 2+ = Mg, M 3+ = Fe3++Al3+). Hence, Fe3+ predominates over Fe2+ in all types of zirconolite, thus enabling the high REE content in Nb-poor zirconolites to be stored in locally dominant REEZrTiFe3+O7 component known so far only as a synthetic analogue of natural zirconolite. Other types of zirconolite from Evate are dominated by the common CaZrTi2O7 end member, but the aforementioned "synthetic" REEZrTiFe3+O7 accompanied by another `synthetic' (U,Th)ZrFe3 + 2O7 component are also abundant. The U,Pb,Th concentrations in U,Th-zirconolites plot discordantly to theoretical isochrons, thus indicating 440 ppm of non-radiogenic excess lead in earlier Nb-rich zirconolite contrasting with secondary Pb loss from later Nb-poor zirconolite. The non-radiogenic Pb-corrected age of the early zirconolite corresponded to 485 ± 9 Ma, within uncertainty limit identical with the 493 ± 10 Ma age of the associated uranothorianite. The variegated chemical composition of zirconolites reflects the complex history of the Evate deposit. Compositional and

  15. Oils of insects and larvae consumed in Africa: potential sources of polyunsaturated fatty acids

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    Womeni Hilaire Macaire

    2009-07-01

    Full Text Available The objective of this paper is to present the beneficial aspects of some insects consumed in sub-Saharan Africa, based on examples of insects consumed in Cameroon, to present their potential as sources of lipids and essential fatty acids. In Africa, termites, larvae of raphia weevil, caterpillars, crickets, bees, maggots, butterflies, weevil, etc. are significant sources of food. These insects belong mainly to the orders of : Isoptera, Orthoptera, Dictyoptera, Coleoptera, Hymenoptera, Lepidoptera and Diptera. Depending on the species, insects are rich in proteins, minerals (K, Ca, Mg, Zn, P, Fe and/or vitamins (thiamine/B1, riboflavine/B2, pyridoxine/B6, acid pantothenic, niacin. The composition of oils extracted from the following six insects consumed in Cameroon was investigated : larvaes of raphia weevil (Rhynchophorus phoenicis, crickets (Homorocoryphus nitidulus, grasshopper (Zonocerus variegates, termites (Macrotermes sp., a variety of caterpillars (Imbrasia sp. and an unidentified caterpillar from the forest (UI carterpillar. The extraction yields of oil were 53.75%, 67.25%, 9.12%, 49.35%, 24.44% and 20.17% respectively for raphia weevil larvae, crickets, devastating crickets, termites, Imbrasia and UI caterpillar. The oil from raphia weevil mainly contains 37.60% of palmitoleic acid and 45.46% of linoleic acid. The oil from crickets is principally made up of palmitoleic acid (27.59%, linoleic acid (45.63% and α-linolenic acid (16.19%. The oil from grasshoppers is composed of palmitoleic acid (23.83%, oleic acid (10.71%, linoleic acid (21.07%, α-linolenic acid (14.76% and γ-linolenic acid (22.54%. The main components of termite oil are : palmitic acid (30.47%, oleic acid (47.52% and linoleic acid (8.79%. Palmitic acid (36.08% and linolenic acid (38.01% are the two dominant fatty acids of Imbrasia oil. As Imbrasia oil, UI caterpillar oil is composed of palmitic acid (30.80% and linolenic acid (41.79%. Stearic acid (7.04%, oleic acid

  16. Analyzing the Dynamics of Communication in Online Social Networks

    Science.gov (United States)

    de Choudhury, Munmun; Sundaram, Hari; John, Ajita; Seligmann, Doree Duncan

    This chapter deals with the analysis of interpersonal communication dynamics in online social networks and social media. Communication is central to the evolution of social systems. Today, the different online social sites feature variegated interactional affordances, ranging from blogging, micro-blogging, sharing media elements (i.e., image, video) as well as a rich set of social actions such as tagging, voting, commenting and so on. Consequently, these communication tools have begun to redefine the ways in which we exchange information or concepts, and how the media channels impact our online interactional behavior. Our central hypothesis is that such communication dynamics between individuals manifest themselves via two key aspects: the information or concept that is the content of communication, and the channel i.e., the media via which communication takes place. We present computational models and discuss large-scale quantitative observational studies for both these organizing ideas. First, we develop a computational framework to determine the "interestingness" property of conversations cented around rich media. Second, we present user models of diffusion of social actions and study the impact of homophily on the diffusion process. The outcome of this research is twofold. First, extensive empirical studies on datasets from YouTube have indicated that on rich media sites, the conversations that are deemed "interesting" appear to have consequential impact on the properties of the social network they are associated with: in terms of degree of participation of the individuals in future conversations, thematic diffusion as well as emergent cohesiveness in activity among the concerned participants in the network. Second, observational and computational studies on large social media datasets such as Twitter have indicated that diffusion of social actions in a network can be indicative of future information cascades. Besides, given a topic, these cascades are often a

  17. Religiosidad y comunicación interespacial en la Edad Media. Los viajes celestiales en el Poema de Santa Oria

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    Cea Gutiérrez, Antonio

    1999-06-01

    Full Text Available The author discusses Gonzalo de Berceo's Poema de Santa Oria, finished around 1252, and analyzes its concept of heaven, with its stratification and hierarchical divisions according to the degree of sanctity of its inhabitants. He also examines the communication between the celestial and terrestrial worlds via the ascents to and descents from heaven that Santa Oria experiences, who otherwise lived locked up between walls from 1043 to 1070. The Vita of this saint from the región of La Rioja, based on the work of the cleric Muño and used by Berceo as a reliable source, reveals a variegated number of elements, from high as well as popular poetry, that accumulated in layers and bear upon ideas and beliefs about the heavenly world. Other themes that Berceo emphasizes are the importance of trustworthiness in the transmission —both oral and written— of the hagiographic texts used as exempla and the direct Information that the saint gave the biographer.A través del Poema de Santa Oria, de Gonzalo de Berceo, se analiza el concepto de cielo, su estratificación y divisiones jerárquicas, atendiendo al grado de santidad de sus habitantes. Se estudia, además, la comunicación entre los mundos celestial y terrenal a través de los viajes al cielo y del cielo —asunciones y descensiones— que experimenta esta santa riojana que vivió emparedada entre 1043 y 1070. Esta Vita, sacada a su vez de la escrita por el clérigo Muño, y que Berceo utiliza como fuente segura, muestra las distintas adherencias cultas y populares, acumuladas en estratos, de lo que se pensaba y creía del mundo celestial como premio. La importancia a la fidelidad en la transmisión —oral y escrita— de los textos hagiográficos que han de servir como exempla es otro de los temas, junto con el de la información directa del santo al biógrafo, en que, llamativamente, hace hincapié el escritor emilianense, que concluyó esta obra hacia el año 1252.

  18. Glacier surge mechanism based on linked cavity configuration of the basal water conduit system

    Science.gov (United States)

    Kamb, Barclay

    1987-08-01

    Based on observations of the 1982-1983 surge of Variegated Glacier, Alaska, a model of the surge mechanism is developed in terms of a transition from the normal tunnel configuration of the basal water conduit system to a linked cavity configuration that tends to restrict the flow of water, resulting in increased basal water pressures that cause rapid basal sliding. The linked cavity system consists of basal cavities formed by ice-bedrock separation (cavitation), ˜1 m high and ˜10 m in horizontal dimensions, widely scattered over the glacier bed, and hydraulically linked by narrow connections where separation is minimal (separation gap ≲ 0.1 m). The narrow connections, called orifices, control the water flow through the conduit system; by throttling the flow through the large cavities, the orifices keep the water flux transmitted by the basal water system at normal levels even though the total cavity cross-sectional area (˜200 m2) is much larger than that of a tunnel system (˜10 m2). A physical model of the linked cavity system is formulated in terms of the dimensions of the "typical" cavity and orifice and the numbers of these across the glacier width. The model concentrates on the detailed configuration of the typical orifice and its response to basal water pressure and basal sliding, which determines the water flux carried by the system under given conditions. Configurations are worked out for two idealized orifice types, step orifices that form in the lee of downglacier-facing bedrock steps, and wave orifices that form on the lee slopes of quasisinusoidal bedrock waves and are similar to transverse "N channels." The orifice configurations are obtained from the results of solutions of the basal-sliding-with-separation problem for an ice mass constituting of linear half-space of linear rheology, with nonlinearity introduced by making the viscosity stress-dependent on an intuitive basis. Modification of the orifice shapes by melting of the ice roof due to

  19. Lessons from Vesta and Ceres

    Science.gov (United States)

    Russell, C. T.; Raymond, C. A.; McSween, H. Y.; Jaumann, R.; DeSanctis, M. C.; Nathues, A.; Prettyman, T.; Capria, M. T.; Pieters, C.; McFadden, L.; Ammannito, E.; Sykes, M. V.; McCord, T. B.; Zuber, M.; Smith, D.; Hoffman, M.; Scully, J. E. C.; Buczkowski, D.

    2014-04-01

    When first discovered, the bodies in the asteroid belt were considered the missing planet(s) between Mars and Jupiter. When their small size and large number become realized, they were deemed to be minor planets and then asteroids. They soon were considered to be simply airless bodies, consisting mostly of rocky material, some having iron cores. When Dawn reached Vesta, this picture was initially largely reinforced by the extensive southern basin and the battered northern hemisphere. A more accurate picture arises, using the color filters of the Framing Camera, in coordination with the nearinfrared spectrometer, revealing a diverse surface with different minerals and processes affecting regions on the surface in various ways. The variegated light and dark material and varying thermal properties indicate a complex surface. The water (OH) content of the surface is far from uniform. Examinations of the floors of Marcia and Cornelia revealed pits, and their crater walls have possibly water-carved gullies. The parent craters appear to have been formed in a wet surface, possibly ice melted in the crater-forming event. Figure 1 shows the latest mosaic of the vestan surface with the currently approved names for the surface features. It had been expected that olivine would be excavated in the southern basin but it was not to be found there. Surprisingly, patches of olivine-rich material were discovered in the north. Doubts arose as to whether a magma ocean hypothesis applies to Vesta, in spite of quantifying the mass of its core, and new ways to explain Vesta's petrogenesis were developed. Closer examination of the surface suggested more interesting scenarios, possible excavation of early volcanic materials, odd craters that seemed impossible to form with simple impacts, and a long ribbon of material stretching diagonally across the surface, possibly originating in the Marcia ejecta blanket. The relative youth of some of these features (ca 50 Ma) suggest Vesta has had

  20. Ingestão de seiva do xilema de laranjeiras 'Pêra' e 'Valência' (Citrus sinensis (L. Osbeck sadias e infectadas por Xylella fastidiosa, pelas cigarrinhas vetoras Oncometopia facialis e Dilobopterus costalimai (Hemiptera: Cicadellidae Xylem sap ingestion form healthy "Pera" and "Valencia" sweet orange (Citrus sinensis (L. Osbeck and infected ones by Xylella fastidiosa, Oncometopia facialis and Dilobopterus costalimai (Hemiptera: Cicadellidae

    Directory of Open Access Journals (Sweden)

    Luiz Henrique Montesino

    2006-08-01

    Full Text Available Estudou-se o efeito da infecção pela bactéria Xylella fastidiosa, agente causal da Clorose Variegada dos Citros (CVC, sobre a taxa de ingestão de seiva do xilema de plantas cítricas por duas espécies de cigarrinhas vetoras (Hemiptera: Cicadellidae. Foram utilizados pés-francos de laranjeira-doce (Citrus sinensis das variedades 'Pêra' e 'Valência', infectadas por X. fastidiosa da linhagem 9a5c, por meio de inoculação mecânica. Os insetos utilizados nos experimentos foram coletados em campo, sendo um representante da Tribo Cicadellini (Dilobopterus costalimai e um da Proconiini (Oncometopia facialis. A taxa de ingestão de seiva do xilema por O. facialis foi quantificada nos ramos das plantas e a de D. costalimai nas folhas e ramos, por meio da avaliação do volume do líquido (honeydew excretado por unidade de tempo. O consumo pela cigarrinha O. facialis nas plantas doentes foi menor do que nas plantas sadias. Na variedade 'Pêra' doente, o consumo foi baixo, não permitindo a quantificação da seiva eliminada. Na 'Pêra' sadia e na 'Valência' doente e sadia, O. facialis apresentou valores expressivos de excreção, com maior alimentação no período diurno. Nas plantas sadias das duas variedades, o consumo pela cigarrinha D. costalimai foi maior do que nas plantas com CVC. Comparando-se as variedades, o consumo foi superior na variedade 'Valência', e, em relação às partes da planta, folha e ramo, a taxa de ingestão foi maior no ramo das duas variedades, apresentando consumo maior no período diurno.It was studied the effect of Xylella fastidiosa infection, causal agent of Citrus Variegated Chlorosis (CVC, on the xylem sap ingestion rate of citrus plants by two sharpshooters species (Hemiptera: Cicadellidae. Seedlings of sweet orange Pera and Valencia (Citrus sinensis were used and infected by X. fastidiosa, strain 9a5c, obtained by mechanical inoculation. The insects used in the experiments were collected in the field, one

  1. Superfícies de resposta do tangor 'Murcott' à fertilização com N, P e K Response models of 'Murcott' tangor to N, P, and K fertilization

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    Dirceu Mattos Junior

    2004-04-01

    Full Text Available O valor de comercialização no mercado de fruta fresca e a maior resistência a doenças, como o declínio e a clorose variegada dos citros, comparado às variedades de laranjas, estimularam a produção de tangerinas e de 'Murcott' na última década. Por outro lado, faltam informações seguras para o diagnóstico das necessidades de adubação para esses cítricos. Assim, o presente trabalho foi planejado com os objetivos de estudar a demanda por nutrientes e estabelecer doses de fertilizantes para maximizar a produtividade e a qualidade dos frutos de tangor 'Murcott' e definir critérios de diagnóstico da análise de folhas para o manejo nutricional dessas plantas. O experimento foi desenvolvido durante seis anos, num pomar comercial da variedade Murcott sobre limoeiro 'Cravo', com 4 anos de idade. Os tratamentos foram arranjados no delineamento fatorial fracionado, do tipo ½ (4³ e constituídos por níveis de N (30; 100; 170 e 240 kg N ha-1, de P (20; 80; 140 e 200 kg ha-1 de P2O5 e de K (30; 110; 190 e 270 kg ha-1 de K2O. O N aumentou a produção média do período, enquanto o efeito do K foi negativo. Não houve efeito significativo para doses de P. A produção máxima de frutos foi obtida com as doses 155; 20 e 30 kg ha-1, respectivamente, de N, P2O5 e K2O, enquanto, para otimizar o tamanho dos frutos, foi necessário elevar a doses de K para 270 kg ha-1 de K2O. Outras características de qualidade dos frutos também foram estudas, bem como critérios para a interpretação de resultados de análises de folhas.The Brazilian production of mandarins and 'Murcott' tangor has increased during the 1990's due to superior value of fruit for commercialization and higher resistance to citrus diseases, i.e.: citrus variegated chlorosis, compared to sweet oranges. On the other hand, there is a lack of information to establish optimum nutrient requirements for such varieties. Therefore, the present work aimed to study the response of 'Murcott

  2. CONTRIBUTO AL DIBATTITO SUL PRINCIPIO DI EGUAGLIANZA DEI MEZZI PROCESSUALI

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    Dejan Bodul

    2017-01-01

    Full Text Available Il principio di eguaglianza dei mezzi processuali presuppone l’esistenza di una ragionevole possibilità in capo ad entrambe le parti di esporre e comprovare i fatti, in un modo che non metta alcuna delle parti in posizione sfavorevole rispetto alla controparte (caso LB INTERFINANZ A.G. v. Croazia, sentenza del 27.03.2008, riscorso n. 29549/04.. Un tanto rappresenta, nella sua espressione di equilibrio tra le parti processuali, uno degli elementi costitutivi del diritto ad un giusto processo. Tuttavia, con l’entrata in vigore della (nuova legge fallimentare (GU, n. 71/15. in avanti LF la questione dell’eguaglianza sul piano processuale diviene nuovamente di attualità. Precisamene, la LF ha mantenuto una soluzione in base alla quale il creditore ha la possibilità di instaurare il procedimento fallimentare qualora renda probabile l’esistenza del proprio credito e la sussistenza del fondamento fallimentare così che è trattenuto ed eccepito il provvedimento in base al quale la probabilità della sussistenza del credito non può venire dimostrata dal creditore sulla base di una decisione dell’autorità giudiziale o amministrativa che non sia passata in giudicato. Posto che esistono diverse soluzioni con riguardo alle questioni illustrate, gli autori si limiteranno a delle constatazioni di carattere generale sulla base di esperienze comparate, senza una dettagliata disamina delle variegate e peculiari soluzioni giuridiche straniere. Nel lavoro si disaminano sia le implicazioni pratiche che quelle teoriche della soluzione giuridica di diritto positivo, analizzando la giurisprudenza della Corte europea dei diritti dell’uomo nei procedimenti in base all’art. 6 (diritto ad un giusto processo della Convenzione europea per la salvaguardia dei diritti dell’uomo e delle libertà fondamentali, poiché partiamo dal presupposto che le conoscenze sul punto possono essere fondamentali per la comprensione dell’oggetto dell’indagine condotta

  3. Arrested diatreme development: Standing Rocks East, Hopi Buttes, Navajo Nation, USA

    Science.gov (United States)

    Lefebvre, Nathalie S.; White, James D. L.; Kjarsgaard, Bruce A.

    2016-01-01

    fluidal, folded-over shapes and ropy surfaces, subordinate thermally altered wall-rock and variegated domains of lapilli tuff. SRE shows a progressive transition from fissure to diatreme, and overall evolution from more explosive to weakly explosive eruption styles recorded at the conduit-crater transition. Diatreme development was initiated by deep-quarrying explosive eruptions along a fissure to form the country rock-rich breccia. Only parts of the fissure remained active as magma feeding the highly explosive eruptions along the fissure localized into discrete point sources forming the matrix-rich lapilli tuff deposits. These superimposed deposits record the passage of multiple debris-jets and subvertical fallback from shallow cratering arising from explosions triggered by magma-water interaction at numerous, discrete sites. However, instead of continuing to build a well-formed diatreme, the system switched to weak spattering with intermittent explosive activity and near-surface dike emplacement into the unconsolidated anisotropic, pyroclastic debris of the crater floor. Dominant spatter from strombolian-style bursts accumulated on the topographically varied, evolving unstable syn-eruptive crater floor, and led to local failure and remobilization. This study demonstrates how the combination of fissure behavior and sensitivity of the shallow plumbing system to local conditions during an eruption can lead to a decrease in eruptive footprint within the diatreme structure, and an overall decrease in explosivity resulting in the arrested development of an immature diatreme.

  4. Valikuid ja võimalusi: ülevaade Uku Masingu luuletõlkimisest. Choices and possibilities: A survey of Uku Masing’s poetry translations

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    Aile Tooming

    2012-06-01

    after publication. The real “(rediscovering” and (republishing of Masing’s works (among them translations began some years after his death, in 1988 with the political liberation of Estonia, and continues to the present day. We can distinguish between those translations that Masing carried out for his own interest (works by Tagore, Shanfara, Omar Khayyam, Matsuo Bashô, Louise Labé et al. and those that were commissioned, such as contributions to anthologies (Anthology of Greek Literature, Anthology of Roman Literature and Anthology of Renaissance Literature. Through his translations, Masing was also searching for something reflected in his own poetry and discussed in his essays. Masing translated poetry from English, German, French, Italian, Ancient Greek, Latin, Hebrew, Persian, Arabic, Sanskrit, Sumerian, Syrian, Coptic and Tigre. The list can perhaps be expanded by a few more entries. The cultures and eras that are the source of the translated texts also form a variegated and extensive tapestry, including ancient Sumer, the Old Testament world, the classical culture of antiquity, the Italian and French Renaissance, and Japanese, Persian and Arabic poetry. In the case of a number of translations, Masing was a trailblazer, since traditions of translation from the given languages and cultures were lacking altogether in Estonia. As a translator, Masing represented the rare combination of scholar (theologian and linguist and poet, which is the key to his meticulousness in translation, as well as his fluid and creative use of language.

  5. Economic culture of the Tuvans within the scope of the sociocultural approach

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    Yuri V. Popkov

    2018-06-01

    Full Text Available In this article, the authors argue that for the periods of social turmoil, understanding culture as a set of norms controlling social behavior is limited and insufficient. A more adequate approach would involve interpreting culture as a specific historical version of the society’s existence in its qualitative form. This hearkens back to the sociocultural approach which postulates the culture as an ensemble, and the sociocultural construed as the social in the cultural variety. In this context, economic culture might be seen as a set of qualitative features of a specific economic entity shaped by the local conditions of development. With all its peculiarities, an economic culture appears an element of this sociocultural diversity. Studying the economic culture of Tuvans through the lens of the sociocultural approach and the notion of this diversity allows us to outline several important aspects of this issue, rarely addressed by the existing academic discourse. A focus should be made, for instance, not only on the dominant nomadic culture, but also on subdominant economic cultures which helped shape a number of skills and techniques. While nomadism helped develop unhurried and contemplative attitude to life, hunting demanded dynamic change, and agriculture and crafts called for diligence and meticulousness. A brief glance at the economic history of Tuvans allowed the authors to conclude that economic diversity both provides stability to the economic culture of Tuvan society and expresses its variegation, its capacity to develop through numerous modifications. This polymorphism acts as an important resource which helps launch the alternate routes of ethnic development. These routes rarely appear self-evident under the dominant type of economic culture which had been shaping the traditional life of Tuvan society. Tapping into this alternative potential can prove an important reserve for future development. An overview of ethnographic data helps

  6. Posição da gema axilar e a indução de mutação em mudas de crisântemo (Dendranthema grandiflora Tzvelev Position of the axillary bud and mutation inducion in chrysanthemun (Dendranthema glandiflora Tzevelev plantets

    Directory of Open Access Journals (Sweden)

    Alvis Hernán Adames

    1999-10-01

    , originated from six different axillary buds from the M1V1 chrysanthemum branches. For this purpose, rooted plants of the cultivar ‘Ingrid’ (dark pink were irradiated with 20 Gy of gamma-rays and the prune was carried out 40 days after planting. Frequency and spectrum of flower color mutants were evaluated. No mutants were observed in the control population. In the M1V2 population, 22.1% of the total plants were mutants (white color, dark bronze, pale pink, yellow, wine, variegated and cream. Among them, 1.8% were periclinal chimeras (with only one different color from the original and the others showed mutated sectors. No differences were observed in mutation frequency and size of mutated sector among six M1V2 populations. The wine colored mutant was selected, multiplied and evaluated in a yield trial. This mutant named ‘Magali’ was multiplied and was released as a new cultivar.

  7. Host colonization differences between citrus and coffee isolates of Xylella fastidiosa in reciprocal inoculation Diferenças em colonização do hospedeiro por isolados de Xylella fastidiosa de citros e cafeeiro em inoculações recíprocas

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    Simone de Souza Prado

    2008-01-01

    Full Text Available Citrus variegated chlorosis (CVC and coffee stem atrophy (CSA are important diseases in Brazil associated with closely-related strains of Xylella fastidiosa, but little is know about host aoverlappingnd importance of citrus and coffee as inoculum sources of these strains. In this study, reciprocal-inoculation experiments were performed to determine if CVC and CSA isolates are biologically similar within citrus and coffee plants. These two hosts were mechanically inoculated with a CVC and a CSA isolate of X. fastidiosa at four concentrations ranging between10³ and 10(9 colony forming units CFU mL-1. At two, four and eight months after inoculation, the infection efficiency and bacterial populations of the isolates in each host were determined by culturing. The CVC isolate infected both citrus and coffee plants, but developed lower populations in coffee. The CSA isolate did not colonize citrus. Inoculation of coffee plants with the CVC isolate resulted in low rates of infection and required an inoculum concentration ten-fold higher than that necessary to obtain a similar (25% rate of infection in citrus. The relatively low infection rates and bacterial numbers of the CVC isolate in coffee plants compared with those observed in citrus suggest that coffee is not a suitable host to serve as a source of inoculum of the CVC strain for primary spread to citrus or within coffee plantations.Clorose variegada dos citros (CVC e atrofia dos ramos do cafeeiro (ARC são doenças importantes no Brasil, associadas a estirpes de Xylella fastidiosa que são geneticamente próximas. Entretanto, pouco se sabe a respeito de plantas hospedeiras em comum e da importância de citros e cafeeiro como fontes de inóculo dessas estirpes. Neste estudo, realizaram-se experimentos de inoculação recíproca para determinar se isolados de X. fastidiosa de CVC e de ARC são biologicamente semelhantes em plantas de citros e café. Estes dois hospedeiros foram mecanicamente

  8. Supergene destruction of a hydrothermal replacement alunite deposit at Big Rock Candy Mountain, Utah: Mineralogy, spectroscopic remote sensing, stable-isotope, and argon-age evidences

    Science.gov (United States)

    Cunningham, Charles G.; Rye, Robert O.; Rockwell, Barnaby W.; Kunk, Michael J.; Councell, Terry B.

    2005-01-01

    Big Rock Candy Mountain is a prominent center of variegated altered volcanic rocks in west-central Utah. It consists of the eroded remnants of a hypogene alunite deposit that, at ∼21 Ma, replaced intermediate-composition lava flows. The alunite formed in steam-heated conditions above the upwelling limb of a convection cell that was one of at least six spaced at 3- to 4-km intervals around the margin of a monzonite stock. Big Rock Candy Mountain is horizontally zoned outward from an alunite core to respective kaolinite, dickite, and propylite envelopes. The altered rocks are also vertically zoned from a lower pyrite–propylite assemblage upward through assemblages successively dominated by hypogene alunite, jarosite, and hematite, to a flooded silica cap. This hydrothermal assemblage is undergoing natural destruction in a steep canyon downcut by the Sevier River in Marysvale Canyon. Integrated geological, mineralogical, spectroscopic remote sensing using AVIRIS data, Ar radiometric, and stable isotopic studies trace the hypogene origin and supergene destruction of the deposit and permit distinction of primary (hydrothermal) and secondary (weathering) processes. This destruction has led to the formation of widespread supergene gypsum in cross-cutting fractures and as surficial crusts, and to natrojarosite, that gives the mountain its buff coloration along ridges facing the canyon. A small spring, Lemonade Spring, with a pH of 2.6 and containing Ca, Mg, Si, Al, Fe, Mn, Cl, and SO4, also occurs near the bottom of the canyon. The 40Ar/39Ar age (21.32±0.07 Ma) of the alunite is similar to that for other replacement alunites at Marysvale. However, the age spectrum contains evidence of a 6.6-Ma thermal event that can be related to the tectonic activity responsible for the uplift that led to the downcutting of Big Rock Candy Mountain by the Sevier River. This ∼6.6 Ma event also is present in the age spectrum of supergene natrojarosite forming today, and probably

  9. Xylella fastidiosa: an examination of a re-emerging plant pathogen.

    Science.gov (United States)

    Rapicavoli, Jeannette; Ingel, Brian; Blanco-Ulate, Barbara; Cantu, Dario; Roper, Caroline

    2018-04-01

    Xylella fastidiosa is a Gram-negative bacterial plant pathogen with an extremely wide host range. This species has recently been resolved into subspecies that correlate with host specificity. This review focuses on the status of X. fastidiosa pathogenic associations in plant hosts in which the bacterium is either endemic or has been recently introduced. Plant diseases associated with X. fastidiosa have been documented for over a century, and much about what is known in the context of host-pathogen interactions is based on these hosts, such as grape and citrus, in which this pathogen has been well described. Recent attention has focused on newly emerging X. fastidiosa diseases, such as in olives. Bacteria; Gammaproteobacteria; family Xanthomonadaceae; genus Xylella; species fastidiosa. Gram-negative rod (0.25-0.35 × 0.9-3.5 μm), non-flagellate, motile via Type IV pili-mediated twitching, fastidious. Xylella fastidiosa has a broad host range that includes ornamental, ecological and agricultural plants belonging to over 300 different species in 63 different families. To date, X. fastidiosa has been found to be pathogenic in over 100 plant species. In addition, it can establish non-symptomatic associations with many plants as a commensal endophyte. Here, we list the four distinct subspecies of X. fastidiosa and some of the agriculturally relevant diseases caused by them: X. fastidiosa ssp. fastidiosa causes Pierce's disease (PD) of grapevine (Vitis vinifera); X. fastidiosa ssp. multiplex causes almond leaf scorch (ALS) and diseases on other nut and shade tree crops; X. fastidiosa ssp. pauca causes citrus variegated chlorosis (CVC) (Citrus spp.), coffee leaf scorch and olive quick decline syndrome (OQDS) (Olea europaea); X. fastidiosa ssp. sandyi causes oleander leaf scorch (OLS) (Nerium oleander). Significant host specificity seemingly exists for some of the subspecies, although this could be a result of technical biases based on the limited number of

  10. Multiannual runoff variability in the upper Danube region

    International Nuclear Information System (INIS)

    Pekarova, P.

    2009-05-01

    The main results and new knowledge of the presented Thesis can be summarized as follows: - The daily mean discharge time series of the river Danube at Bratislava has been supplemented with data from years 1876-1890. So it has been extended into the full-uninterrupted daily data series for the length of 130 years. Such series of the daily mean discharges is suitable for statistical analyses of the hydrological characteristics changes, and also for identification of the multi-annual (up to 30- years) cycles. - Using the combined periodogram method and the series filtration, in the discharge time series, the variegation cycles of dry and wet time periods were searched. For such objective, the time series were used, of more than 100 rivers of the world. From the longer cycles in the series the approximately 28-30 and 20-22 yrs. cycles of the dry and wet periods occurrence were identified. However, the cycle lengths is not exactly 28 and 21 years, but in the long term average, they come close to these values. From the shorter cycles, those with 7.8-, 6.5-, 5.2-; 4.14-, 3.65, and 2.4- years were identified. - These periods were documented for the discharge series of the analyzed rivers in various physiographic latitudes of the world. It is then possible to consider this piece of knowledge as generally valid for the whole Earth. These periods are connected with the Solar activity and with the thermohaline circulation (ocean conveyor belt). Their parts are also the El Nino, AO, NAO, and QBO phenomena. - Through the cross-correlations (two variables), time shift of the dry and wet periods was identified depending upon the geographical longitude and latitude of the river basins location. From these results it follows, that the dry and wet periods do not occur at the same time on various locations. This time shift depends upon the basins location, and it follows upon the shift in the precipitations occurrence. - The trend analysis did not indicate any significant trends in

  11. Produtividade e qualidade dos frutos da laranjeira 'Pêra' clone IAC em 16 porta-enxertos na região de Bebedouro-SP Yield and fruit quality of 'Pêra' sweet orange clone IAC on 16 rootstocks in Bebedouro region, State of São Paulo, Brazil

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    Eduardo Sanches Stuchi

    2004-08-01

    and chemical fruit characteristics such as fruit size, total soluble solids, acidity, ratio juice content and technological index of 'Pera' IAC sweet orange [Citrus sinensis (L. Osbeck], in a high inoculum pressure area of citrus variegated chlorosis (CVC. The spacement was 6.0 m between rows and 3.5 m between trees. The experimental design used was randomized blocks, with three replications and two trees per plot. The rootstocks tested were 'Sun Chu Sha Kat' mandarin (Citrus reticulata, 'Pectinífera' (C. reticulata, 'Shekwasha' (C. depressa Hayata, 'Pectinífera/Shekwasha' (C. depressa Hayata, 'Batangas' (C. reticulata, 'Oneco' (C. reticulata, citrangor [citrange (Poncirus trifoliata Raf. x C. sinensis x C. sinensis], citrandarin (C. sunki hort. ex Tanaka x Poncirus trifoliata L. Raf. cv. English, 'Sunki' (C. sunki, 'Suen-Kat' (C. sunki, Nasnaran (C. amblycarpa Ochse, 'Venezuela' mandarin (C. reticulata, 'Heen Naran' mandarin (C. lycopersicaeformis, 'Cravo' (C. limonia Osbeck x 'Cleopatra' (C. reshni hort ex Tanaka, 'Cravo' (C. limonia, 'Cleopatra' (C. reshni. CVC intensity was different due to rootstocks effects and not related with yield until the fourth year of production. With the exception of the Nasnaran mandarin the rootstocks induced initial fruit production and quality similar to 'Cravo'.

  12. Book Review:

    Science.gov (United States)

    Hudson, R. P.

    1981-04-01

    example, time and frequency) are accorded several lectures and lecturers, while most receive only one each. That choice by the conference's organizers is not explained in the Foreword. But it is not a very serious drawback; nor—for the anglophone reader, at least—is the appearance here and there of quaint inventions in English which, in fact, add to the charm. There are short articles on the Determination of Atomic Masses of Nuclides (Wapstra), some Problems in Photometry (Korte); two by A Bray on Force Standards, one dealing with Dissemination and the other with Measurement of "g"; Time Scales (Leschiutta); determining the Volume of a Sphere (Terrien); and two by Giacomo, one commenting on Mass Measurements and one discussing the Speed of Light. Of intermediate length are reviews of the Determination of Best Values of the Fundamental Physical Constants (Cohen); Length Measurement Standards (Giacomo), and Topics in Quantum Electrodynamics (Combley and Picasso). The extended treatment of time and frequency metrology includes three major articles by Audoin: a general (largely analytical) one on Frequency Metrology, followed by detailed discussions of Cesium Beam and Hydrogen Maser technology. There are, in addition, specialized treatments of Optically-Pumped Microwave Devices (Arditi) and of Optical Frequency Standards (i.e., lasers) by Chebotayev; finally, a brief note by De Marchi on Problems in Frequency Synthesis in the far Infrared Region. A long article by Petley covers the many-faceted subject of Electrical Metrology and the Fundamental Constants. Equally variegated, although belied by its simple title, is a discussion of Thermometry by Quinn. And last, but not least, is a detailed account by Deslattes of his determination of Avogadro's Constant which ranges over the topics of Infrared to Gamma-ray Reference Wavelengths, Mass and Density. In summarizing it is difficult to avoid the assertion, however hackneyed, that no physicist can afford to be without—or, at

  13. USING OF THE MATHEMATICAL STATISTICS METHODS FOR THE CHARACTERISTIC OF THE ELEMENTAL CONTAMINATION URBAN ECOSYSTEMS SOILS BY THE HEAVY METALS

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    YAKOVYSHYNA T. F.

    2017-03-01

    2010 has been used to determine the patterns of Zn distribution of the across the territory. Conclusion. The using of the mathematical statistics methods for characterizing data sets, as primary values, has been scientifically confirmed in the block for estimating the ecomonitoring system of the heavy metals contamination of the urban ecosystems soils. The intensive technogenic Zn contamination soils of the whole Dnieper territory according to the total content and the increase in the contamination variegation by the potentially available and available forms have been revealed using statistical methods and in the rationing of the sanitary- hygienic (MAC and ecological indicators (natural geochemical background. A close correlation has been established between the total Zn content in the soils of Dnieper urban ecosystem and the potentially available forms, in contrast to the available forms, where it has been not observed, which indicates a selective loss of buffer mechanisms relative to Zn.

  14. Locais e período de alimentação da cigarrinha vetora de Xylella fastidiosa, Bucephalogonia xanthophis (Berg (Hemiptera: Cicadellidae, em mudas cítricas Feeding sites and food intake of Bucephalogonia xanthophis (Berg (Hemiptera: Cicadellidae, a sharpshooter vector of Xylella fastidiosa, on citrus plants

    Directory of Open Access Journals (Sweden)

    Marcelo Pedreira de Miranda

    2008-12-01

    Full Text Available A cigarrinha Bucephalogonia xanthophis (Berg (Hemiptera: Cicadellidae é um importante vetor da bactéria Xylella fastidiosa, agente causal da clorose variegada dos citros. Este trabalho teve como objetivo identificar o local preferido de alimentação e o período de maior atividade alimentar desta cigarrinha em citros, no sentido de elucidar o comportamento alimentar relacionado à transmissão da bactéria. O local de alimentação foi estudado em ensaio de escolha, no qual 30 insetos adultos foram liberados em gaiolas de observação (n = 10 contendo uma muda de laranja-doce [Citrus sinensis (L. Osbeck]. Após 1; 15; 21; 25; 39; 45 e 49 h da liberação, contaram-se os insetos na parte superior (ramos com brotações e inferior (haste principal, até H"40 cm de altura da muda. Nos ramos da parte superior, avaliou-se a preferência entre a haste, o pecíolo e o limbo foliar. Em um segundo ensaio, 20 machos e 20 fêmeas de B. xanthophis foram confinados individualmente sobre a haste de 'seedlings' de laranja-doce para determinar os períodos de alimentação, quantificando-se a excreção de 'honeydew' (medida indireta da ingestão em períodos sucessivos de dia e noite, durante 48 h. A maioria dos indivíduos de B. xanthophis preferiu a haste dos ramos novos (62%, na parte superior da muda cítrica (91%. Nos 'seedlings', observou-se maior volume de excreção e proporção de indivíduos excretando durante a fotofase, independentemente do sexo. Portanto, em estudos de transmissão de X. fastidiosa, deve-se considerar a preferência de B. xanthophis pela haste de brotações cítricas e sua maior atividade alimentar durante a fotofase.The sharpshooter Bucephalogonia xanthophis (Berg (Hemiptera: Cicadellidae is an important vector of Xylella fastidiosa, a bacterium that causes citrus variegated chlorosis. This study aimed to identify the preferred feeding sites and periods of this vector on citrus, in order to improve our understanding of the

  15. Monitoramento populacional das cigarrinhas vetoras de Xylella fastidiosa, através de armadilhas adesivas amarelas em pomares comerciais de citros Population monitoring of leafhopper vectors of Xylella fastidiosa, through yellow sticky traps in commercial citrus orchards

    Directory of Open Access Journals (Sweden)

    Rúbia de Oliveira Molina

    2010-12-01

    Full Text Available A Clorose variegada dos citros (CVC é uma doença causada pela bactéria de xilema Xylella fastidiosa Wells. A disseminação ocorre por meio de insetos vetores pertencente à ordem Hemiptera, família Cicadellidae (subfamília Cicadellinae, os quais transmitem a bactéria depois de se alimentarem em plantas contaminadas. Neste trabalho, objetivou-se identificar e monitorar as espécies de cigarrinhas vetoras em um pomar comercial no município de Paranavaí, Paraná. O experimento foi realizado em um talhão comercial de laranja doce (Citrus sinensis (L. Osbeck, variedade Pera, com 1.000 plantas de dez anos de idade. A amostragem foi realizada por meio de armadilhas adesivas amarelas, distribuídas na área periférica e central do pomar, com duas repetições por rua amostrada. As etiquetas foram distribuídas entre a 5ª e 30ª plantas em 10 ruas, totalizando 20 armadilhas que foram renovadas no pomar, a cada trinta dias, durante o período de avaliação que foi entre junho de 2005 e setembro de 2006. As principais espécies de Cicadellinae capturadas foram Acrogonia citrina Marucci & Cavichioli, Dilobopterus costalimai Young e Macugonalia cavifrons Stal. Essas espécies apresentaram ocorrência constante e frequência de 3,97%, 4,2%, 13,0% respectivamente, em relação ao total de cigarrinhas coletadas.Citrus variegated chlorosis (CVC is the disease caused by the bacteria Xylella fastidiosa Wells. Dissemination occurs through insect vectors belonging to the order Hemiptera, family Cicadellidae (subfamily Cicadellinae, which transmit the bacteria after feeding on infected plants. The objective of this study was to identify the species of insect vectors in an orchard in the municipality of Paranavaí, in the State of Paraná. The experiment was conducted in a commercial stand of sweet orange (Citrus sinensis (L. Osbeck, Pêra variety with 1,000 10-year-old plants. Monitoring was performed using yellow sticky traps, distributed in the central

  16. Reação de variedades e clones de laranjas a Xylella fastidiosa Reaction of oranges varieties and clones to Xylella fastidiosa

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    Paulo Sergio de Souza

    2006-04-01

    Full Text Available A clorose variegada dos citros (CVC é uma doença grave, causada por Xylella fastidiosa. As medidas usuais de controle mostram-se pouco eficientes ou práticas e com alto custo. Dessa forma, o uso de variedades resistentes e/ou tolerantes desponta como a alternativa mais eficiente, razão pela qual se julgou oportuna a realização deste trabalho. O presente estudo teve como objetivo avaliar o comportamento de variedades e clones de laranjas introduzidas em relação a X. fastidiosa. Foram estudados 59 variedades e clones de laranjas doces e 2 de laranjas azedas introduzidos da França, Itália e Portugal. O delineamento experimental foi o de blocos casualizados (DBC, com 62 tratamentos e 4 repetições, incluindo a variedade 'Pêra', como padrão. Cada parcela continha duas plantas, sendo uma inoculada e a outra sem inoculação. Para a inoculação do patógeno, foi empregado o método de encostia, utilizando-se de mudas infectadas. Para a avaliação da incidência da doença, utilizou-se de dados qualitativos, positivos ou negativos, enquanto para severidade empregou-se escala de notas, que foi estabelecida baseando-se nos sintomas de CVC, confirmados através dos testes de PCR. As variedades de laranjas azedas Beja e Sr. Pinto e as laranjas doces Navelina ISA 315, Navelina SRA 332 e Newhall Navel SRA 343 não apresentaram sintomas em folhas até 27 meses após a inoculação.Citrus variegated chlorosis (CVC is a serious disease, caused by Xylella fastidiosa, being the most important to the Brazilian citriculture. The usual measures of control show less efficient or practice and they are very expensive. The use of resistant varieties is showed as the most efficient, consisting the aimed of this research. The objective of the present work was study the behavior of varieties and clones introduced in relation to X. fastidiosa. Were studied 59 varieties and clones of sweet oranges and 2 sour oranges introduced from France, Italy and Portugal

  17. Spatial-temporal distribution of sharpshooters (Hemyptera: Cicadellidae insect vectors of Xylella fastidiosa in citrus orchards = Distribuição espaço-temporal de cigarrinhas (Hemiptera: Cicadellidae vetores da Xylella fastidiosa em pomares cítricos

    Directory of Open Access Journals (Sweden)

    Rúbia de Oliveira Molina

    2016-07-01

    Full Text Available Variegated chlorosis (CVC is a citrus disease, reported initially in the northwest of São Paulo state and in the Triângulo Mineiro region of Minas Gerais state in 1987. The CVC is caused by the xylematic bacteria Xylella fastidiosa. The bacteria is spread through contaminated bubbles or by insect vectors belonging to the Hemyptera order and Cicadellidae family. The aimed of this study was to identify the species of Xylella fastidiosa insect vector and to determine its spatial and temporal distribution in commercial orchards of sweet orange [Citrus sinensis (L. Osbeck]. The experiment was conducted in a commercial area of sweet orange, Pêra variety, grafted on Rangpur lime, located in northwest Paraná. For sampling, yellow sticky traps were used, distributed in the peripheral and central area of the orchard with four replicates per street sampled (5, 30, 55 and 80th plant, each plant was considered a sample unit. Were evaluated ten plots per street, totaling 40 traps for sampling. Every thirty days during the evaluation period, the traps were renewed in the orchard. The main species caught were Acrogonia citrine and Dilobopterus costalimai. The highest incidences occurred from winter to spring, and summer to autumn of the next year. According to the geostatistical analysis, the spatial distribution of these species concentrated in the peripheral zone of the portion where a higher incidence of these species was captured. The results show that it is necessary to adopt pest management practices for the Cicadellidae vector of X. fastidiosa differentiated in space and time. = A clorose variegada dos citros (CVC é uma doença de plantas cítricas, constatada, em 1987, inicialmente nos municípios do noroeste paulista e da região do triângulo mineiro. Ela é causada por uma bactéria de xilema, denominada Xylella fastidiosa. Sua disseminação ocorre através de borbulhas contaminadas ou por meio de insetos vetores da ordem Hemiptera e fam

  18. 'I'm a consumer, I'm not a scientist': Cultivating Student Domain Identification, Agency, and Affect through Engagement in Scientific Practices

    Science.gov (United States)

    Scalone, Giovanna

    This study investigates the potential benefits of redesigning hands-on, commercial inquiry science kits for fifth grade that afford agency and the development of science identities by leveraging youth's interests, personal or shared concerns, challenges or desires. Science identification is considered in relation to learning processes of being, becoming, knowing and doing. As identities are constructed dialogically through engagement, emotion, intentionality, innovation, and solidarity, students' agency is mediated and conceptualized as it develops in practice. The study is introduced in Chapter 1 by acknowledging how agency and identity are constructed from an ideological frame, thus problematizing the current neo-liberal policies undergirding educational reform. The conceptual argument in Chapter 2 outlines a theoretical synthesis of agency and learning. Subsequently, I leveraged a theory of semiosis to highlight how these perspectives on agency and identity contribute to the meaning-making processes of language, culture, and mind. Finally, conceptualizations of agency and identity are mapped to the sociology of scientific knowledge perspective. Chapter 3 situates the study context within a design-based implementation research model where the existing science curriculum units serve as comparisons (Inquiry group) to the experimental units (Agency group). The findings first demonstrate how student and teacher positioning are revealed during the turns of exchange by using functional grammar as a method to analyze how discourse works to construe experience and enact social relationships. Secondly, I analyze youth positioning across conditions highlighting the importance of raising student consciousness to the variegated ways scientists practice science and inducts students into how scientists intentionally and purposefully employ genres to engage in scientific ways of communicating. Student's perspectives, positioning, and emotional investments are then analyzed

  19. Volcanogenic Sulfur on Earth and Io: Composition and Spectroscopy

    Science.gov (United States)

    Kargel, J.S.; Delmelle, P.; Nash, D.B.

    1999-01-01

    The causes of Io's variegated surface, especially the roles of sulfur, and the geochemical history of sulfur compounds on Io are not well understood. Suspecting that minor impurities in sulfur might be important, we have investigated the major and trace element chemistry and spectroscopic reflectance of natural sulfur from a variety of terrestrial volcanic-hydrothermal environments. Evidence suggests that Io may be substantially coated with impure sulfur. On Earth, a few tenths of a percent to a few percent of chalcophile trace elements (e.g., As and Se) comonly occur in sulfur and appear to stabilize material of yellow, brown, orange, and red hues, which may persist even at low temperatures. Percentage levels of chalcophile impurities are reasonably expected to occur on Io in vapor sublimate deposits and flows derived from such deposits. Such impurities join a host of other mechanisms that might explain Io's reds and yellows. Two-tenths to two percent opaque crystalline impurities, particularly pyrite (FeS2), commonly produces green, gray, and black volcanic sulfur on Earth and might explain areas of Io having deposits of these colors. Pyrite produces a broad absorption near 1 ??m that gradually diminishes out to 1.6 ??m - similar but not identical to the spectrum of Io seen in Galileo NIMS data. Percentage amounts of carbonaceous impurities and tens of percent SiO2 (as silicates) also strongly affect the spectral properties of Earth's sulfur. Io's broad absorption between 0.52 and 0.64 ??m remains unexplained by these data but could be due to sodium sulfides, as suggested previously by others, or to As, Se, or other impurities. These impurities and others, such as P and Cl (which could exist on Io's surface in amounts over 1% that of sulfur), greatly alter the molecular structure of molten and solid sulfur. Minor impurities could impact Io's geology, such as the morphology of sulfur lava flows and the ability of sulfur to sustain high relief. We have not found

  20. Effect of land use change on soil properties and functions

    Science.gov (United States)

    Tonutare, Tonu; Kõlli, Raimo; Köster, Tiina; Rannik, Kaire; Szajdak, Lech; Shanskiy, Merrit

    2014-05-01

    For good base of sustainable land management and ecologically sound protection of soils are researches on soil properties and functioning. Ecosystem approach to soil properties and functioning is equally important in both natural and cultivated land use conditions. Comparative analysis of natural and agro-ecosystems formed on similar soil types enables to elucidate principal changes caused by land use change (LUC) and to elaborate the best land use practices for local pedo-ecological conditions. Taken for actual analysis mineral soils' catena - rendzina → brown soils → pseudopodzolic soils → gley-podzols - represent ca 1/3 of total area of Estonian normal mineral soils. All soils of this catena differ substantially each from other by calcareousness, acidity, nutrition conditions, fabric and humus cover type. This catena (representative to Estonian pedo-ecological conditions) starts with drought-prone calcareous soils. Brown (distributed in northern and central Estonia) and pseudopodzolic soils (in southern Estonia) are the most broadly acknowledged for agricultural use medium-textured high-quality automorphic soils. Dispersedly distributed gley-podzols are permanently wet and strongly acid, low-productivity sandy soils. In presentation four complex functions of soils are treated: (1) being a suitable soil environment for plant cover productivity (expressed by annual increment, Mg ha-1 yr-1); (2) forming adequate conditions for decomposition, transformation and conversion of fresh falling litter (characterized by humus cover type); (3) deposition of humus, individual organic compounds, plant nutrition elements, air and water, and (4) forming (bio)chemically variegated active space for soil type specific edaphon. Capacity of soil cover as depositor (3) depends on it thickness, texture, calcareousness and moisture conditions. Biological activity of soil (4) is determined by fresh organic matter influx, quality and quantity of biochemical substances and humus

  1. Estonian soil classification as a tool for recording information on soil cover and its matching with local site types, plant covers and humus forms classifications

    Science.gov (United States)

    Kõlli, Raimo; Tõnutare, Tõnu; Rannik, Kaire; Krebstein, Kadri

    2015-04-01

    cover type); (iii) being compartment for deposition of humus, individual organic compounds, plant nutrition elements, air and water, and (iv) forming (bio)chemically variegated active space for soil type specific edaphon. For studying of ESC matching with others ecosystem compartments classifications the comparative analysis of corresponding classification schemas was done. It may be concluded that forest and natural grasslands site types as well the plant associations of forests and grasslands correlate (match) well with ESC and therefore these compartments may be adequately expressed on soil cover matrixes. Special interest merits humus cover (in many countries known as humus form), which is by the issue natural body between plant and soil or plant cover and soil cover. The humus cover, which lied on superficial part of soil cover, has been formed by functional interrelationships of plants and soils, reflects very well the local pedo-ecological conditions (both productivity and decomposition cycles) and, therefore, the humus cover types are good indicators for characterizing of local pedo-ecological conditions. The classification of humus covers (humus forms) should be bound with soil classifications. It is important to develop a pedocentric approach in treating of fabric and functioning of natural and agro-ecosystems. Such, based on soil properties, ecosystem approach to management and protection natural resources is highly recommended at least in temperate climatic regions. The sound matching of soil and plant cover is of decisive importance for sustainable functioning of ecosystem and in attaining a good environmental status of the area.

  2. Field Insect Pests and Crop Damage Assessment of Pigeon Pea (Cajanus cajan [L.] Huth grown under Ratoon and in Mixture with Maize Evaluación de Campo de Insectos Pestes y Daño del cultivo de Gandul (Cajanus cajan [L.] Huth creciendo bajo Rebrotes o en Mezcla con Maíz

    Directory of Open Access Journals (Sweden)

    Madang A Dasbak

    2012-03-01

    Full Text Available The widespread adoption of cropping systems that are sustainable and environmentally friendly is vital for the macroeconomic survival of civilization. Intercropping could ensure stability of insect populations in a system. A 3-yr (2005-2007 field trial was therefore carried out in Nigeria under regular and ratoon crops to evaluate five recently developed pigeon pea (Cajanus cajan [L.] Huth genotypes (ICPL 87, ICPL 161, ICPL 85063, ICP 7120, and ICPL 87119 from the International Crops Research Institute for Semi-Arid Tropics (ICRISAT and one local variety for their relative performance and susceptibility to insect pests. The pigeon pea genotypes were in a mixture with two maize genotypes (Zea mays L., open-pollinated and hybrid in regular crops for 2 yr (2005-2006 followed by a ratoon crop for 1 yr (2007. Termites (Odontotermes badius, crickets (Gymnogryllus lucens, and variegated grasshoppers (Zonocerus variegatus were the crop's seedling pests and caused minimal damage. Clavigralla spp. infestations were high at the reproductive stage causing 24% and 29% seed damage in regular and ratoon pigeon pea crops, respectively. Maize slightly suppressed insect pest incidence and damage to pigeon pea pods and seeds but significantly (P La extendida adopción de sistemas de cultivo que son sustentables y benignos para el medio ambiente es vital para la supervivencia macro-económica de la civilización. El intercultivo podría asegurar la estabilidad de población de insectos en un sistema. Un ensayo de campo de 3 anos (2005-2007 fue realizado en Nigeria bajo condiciones de cultivo regular y ratoon para evaluar cinco genotipos de poroto gandul (Cajanus cajan [L.] Huth: ICPL 87, ICPL 161, ICPL 85063, ICP 7120, e ICPL 87119 desde International Crops Research Institute for Semi-Arid Tropics (ICRISAT y una variedad local, por sus rendimientos y susceptibilidades relativas a insectos plaga. Los genotipos de poroto gandul estaban en mezcla con dos genotipos de

  3. Geology and ground-water resources of Goshen County, Wyoming; Chemical quality of the ground water

    Science.gov (United States)

    Rapp, J.R.; Visher, F.N.; Littleton, R.T.; Durum, W.H.

    1957-01-01

    Goshen County, which has an area of 2,186 square miles, lies in southeastern Wyoming. The purpose of this study was to evaluate the ground-water resources of the county by determining the character, thickness, and extent of the waterbearing materials; the source, occurrence, movement, quantity, and quality of the ground water; and the possibility of developing additional ground water. The rocks exposed in the area are sedimentary and range in age from Precambrian to Recent. A map that shows the areas of outcrop and a generalized section that summarizes the age, thickness, physical character, and water supply of these formations are included in the report. Owing to the great depths at which they lie beneath most of the county, the formations older than the Lance formation of Late Cretaceous age are not discussed in detail. The Lance formation, of Late Cretaceous age, which consists mainly of beds of fine-grained sandstone and shale, has a maximum thickness of about 1,400 feet. It yields water, which usually is under artesian pressure, to a large number of domestic and stock wells in the south-central part of the county. Tertiary rocks in the area include the Chadron and Brule formations of Oligocene age, the Arikaree formation of Miocene age, and channel deposits of Pliocene age. The Chadron formation is made up of two distinct units: a lower unit of highly variegated fluviatile deposits that has been found only in the report area; and an upper unit that is typical of the formation as it occurs in adjacent areas. The lower unit, which ranges in thickness from a knife edge to about 95 feet, is not known to yield water to wells, but its coarse-grained channel deposits probably would yield small quantities of water to wells. The upper unit, which ranges in thickness from a knife edge to about 150 feet, yields sufficient quantities of water for domestic and stock uses from channel deposits of sandstone under artesian pressure. The Brule formation, which is mainly a

  4. Vertisoles hidromórficos de la planicie costera del Río de La Plata, Argentina Hydromorphic vertisols of the Rio de La Plata coastal plain, Argentina

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    Perla Imbellone

    2011-12-01

    .Hydromorphic Vertisols are the dominant soils in the coastal plain of the la Plata river, Buenos Aires province, Argentina. Four repre-sentative soils in the districts of La Plata, Ensenada and Berazategui were studied. Pedological and sedimentological properties are described and analyzed. The purpose of this contribution is to discuss: a the grain size distribution and mineralogy of hydromorphic Vertisols in the rio de la Plata «mudflat»; b the influence of these properties on the generation of morphological features and c the associated pedogenic processes that take place. The morphology of the soil is related to vertic and hydromorphic features in the upper and middle horizons of the soil. Moderate and strong wedge-shaped and angular aggregates are abundant in the solum, with interpedal slickensides, sometimes striated, visible at various levels of observation. The matrix can be moderately or dominantly anisotropic and shows zones of optical anisotropism that run through the fine material with variegated patterns or anisotropic aureoles surrounding the pores. Hydromorphism is favoured by frequent pluvial flooding, the level landscape and the high water table, which is generally between 0.80 and 1.20 m deep. Therefore, endosa-turation or episaturation can occur at different times of the year. Soils have a variety of redoximorphic features, mainly reduced matrices (2.5Y, 5Y hues; chromas of 2 or less, redox concentrations of manganese and iron as nodules and concretions, surface impregnations of sesquioxides on the peds and some dark subsurface accumulations and ramifications through the matrix; there are also some depleted zones in the matrix. Concretions are hard in hand specimens and occur within the matrix; segregations are soft in hand specimens and they appear in thin sections as impregnations of the matrix with diffuse boundaries. They appear as mottles in the field. Soil textural class is clay. Fine clay markedly predominates over coarse clay. Soils are illitic Vertisols

  5. Obituary: Raymond Edwin White Jr., 1933-2004

    Science.gov (United States)

    Liebert, James William

    2004-12-01

    traditions. Earlier in 1971-72, Ray served as Program Officer for Stars and Stellar Evolution in the Astronomy Section of the National Science Foundation. Ray was one of the three "originators" of "The Inspiration of Astronomical Phenomena" (INSAP) Conferences. These conferences provide scholarly discussions on the many and variegated cultural impacts of the perceptions about the day- and night-time sky, thus providing a forum for a broad sampling of artists, historians, philosophers, and scientists to get together, compare notes, and ask questions of one another. The INSAP Conferences have taken place near Castel Gandolfo Italy, on the island of Malta, near Palermo Italy, and at Oxford University in England. Ray's scholarship also was manifest in his activities as editor. For some years in the 1990s, he edited two astronomy journals, The Astronomy Quarterly and Vistas in Astronomy. Raymond E. White, Jr., is survived by his wife Ruby E. (nee Fisk), his high school sweetheart at Heidelberg High in Germany. Their children include Raymond E. White III (Professor of Physics and Astronomy at the University of Alabama, Tuscaloosa), Kathleen M. (White) Wade, and Kevin D. White. Ray was proud of two beautiful granddaughters, Charlotte R. Wade and Sarah E. Wade. Ray was proud of his early role with Steward Observatory Director Bart Bok in the commissioning of the "90-inch" reflector at the University of Arizona site on Kitt Peak in 1969. He built the direct camera, and was invited by his close friend Bok to share the "first light" of this telescope, now renamed the Bok 2.3-m telescope. When Professor Bok passed away, the astronomy magazine Sky & Telescope invited Ray to write an article which was entitled "Bart J. Bok (1906-83): Personal Memoir from a Grandson." (Bok mentored Ivan R. King, who was Ray's thesis advisor.) In his concluding remarks, Ray wrote, "The aspect of Bart J. Bok I will miss the most is his exuberance for the art of astronomy." We will also miss greatly this

  6. Origin of marls from the Polish Outer Carpathians: lithological and sedimentological aspects

    Science.gov (United States)

    Górniak, Katarzyna

    2012-10-01

    evolution of the Outer Carpathian basin. Marls initiate sedimentation, indicate reconstruction stages and are closing the deposition in the Outer Carpathian basin (Fig. 1). Marls appear in the Polish part of Outer Carpathians in Upper Jurassic, initiating sedimentation in the northern Tethyan domain. Subsequently, they occur within Upper Cretaceous sandy-shaly flysch, indicating the reconstitution stage of Outer Carpathian basin and from Eocene to Oligocene are completing the deposition in successively closing basins (Fig. 1). The appearance of marls indicates the stages of tectonic evolution of the Outer Carpathians. The opening and reconstitution of a basin is accompanied by appearance of marls distinguished as preorogenic (Goleszów, Siliceous-Fucoid, Węgierka. Frydek and Żegocina Marls), their closing - synorogenic marls (Lącko, Budzów, Zembrzyce, Leluchów, Niwa, Grybów, Sub-Cergowa and Dynów Marls). Marls represent sediments redeposited from shelves to deeper parts of basins in the form of muds (M, MT and MS facies) and as olistostromes and olistoliths (MyG facies) (Tables 19, 20). Marls redeposited in the form of olistoliths appear in the stage of opening of the Outer Carpathian basin on the boundary of the Jurassic/Cretaceous period (Goleszow Marls) and in the stage of its Upper Cretaceous reconstitution (Baculite and Zegocina? Marls). In the complexes containing redeposited marls in the form of muds, submarine slumps occur (Table 19). These features indicate tectonic disquiet accompanying deposition of marls. Among the marls studied, dark coloured rocks appear (black, bluish-gray, greenish-gray) and olive and light- coloured (creamy, beige), as well as variegated and red (Table 19). The differentiation of colours indicates sedimentation of Carpathian marls both in oxygenated environments and those that are oxygen-depleted. The analysis of evolution of the Carpathian basins indicates that they were starved basins during sedimentation of marls. Limited supply

  7. Geology and fluorspar deposits, Northgate district, Colorado

    Science.gov (United States)

    Steven, Thomas A.

    1960-01-01

    The fluorspar deposits in the Northgate district, Jackson County, Colo., are among the largest in Western United States. The mines were operated intermittently during the 1920's and again during World War II, but production during these early periods of operation was not large. Mining was begun on a larger scale in 1951, and the district has assumed a prominent position among the fluorspar producers in the United States. Within the Northgate district, Precambrian metamorphic and igneous rocks crop out largely in the Medicine Bow Mountains, and later sedimentary rocks underlie North Park and fill old stream valleys in the mountains. The metamorphic rocks constitute a gneiss complex that formed under progressively changing conditions of regional metamorphism. They consist principally of hornblende-plagioclase gneiss (hornblende gneiss), quartz monzonite gneiss, pegmatite, biotite-garnet-quartz-plagioclase gneiss (biotite-garnet gneiss), hornblende-biotite-quartz-plagioclase gneiss (hornblende-biotite gneiss) and mylonite gneiss. The igneous rocks comprise some local fine-grained dacite porphyry dikes near the west margin of the district, and a quartz monzonitic stock and associated dikes in the central and eastern parts of the district. The sedimentary rocks in the district range in age from Permian to Recent. Folded Permian and Mesozoic rocks underlie the basin of North Park, and consist in sequence from oldest to youngest, of Satanka(?) shale (0-50 feet of brick-red shale) and Forelle(?) limestone (8-15 feet of pink to light-gray laminated limestone) of Permian age, Chugwater formation of Permian and Triassic age (690 feet of red silty shale and sandstone), Sundance formation of Late Jurassic age (145 feet of sandstone containing some shale and limestone), Morrison formation of Late Jurassic age (445 feet of variegated shale and minor sandstone and limestone), Dakota group as used by Lee (1927), now considered to be of Early Cretaceous age in this area (200