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Sample records for variable speed single

  1. Measure Guideline. Replacing Single-Speed Pool Pumps with Variable Speed Pumps for Energy Savings

    Energy Technology Data Exchange (ETDEWEB)

    Hunt, A. [Building Media and the Building America Retrofit Alliance (BARA), Wilmington, DE (United States); Easley, S. [Building Media and the Building America Retrofit Alliance (BARA), Wilmington, DE (United States)

    2012-05-01

    This measure guideline evaluates potential energy savings by replacing traditional single-speed pool pumps with variable speed pool pumps, and provides a basic cost comparison between continued uses of traditional pumps verses new pumps. A simple step-by-step process for inspecting the pool area and installing a new pool pump follows.

  2. Measure Guideline: Replacing Single-Speed Pool Pumps with Variable Speed Pumps for Energy Savings

    Energy Technology Data Exchange (ETDEWEB)

    Hunt, A.; Easley, S.

    2012-05-01

    The report evaluates potential energy savings by replacing traditional single-speed pool pumps with variable speed pool pumps, and provide a basic cost comparison between continued uses of traditional pumps verses new pumps. A simple step-by-step process for inspecting the pool area and installing a new pool pump follows.

  3. Variable speed generators

    CERN Document Server

    Boldea, Ion

    2005-01-01

    With the deregulation of electrical energy production and distribution, says Boldea (Polytechnical Institute, Timisoara, Romania) producers are looking for ways to tailor their electricity for different markets. Variable-speed electric generators are serving that purpose, up to the 400 megavolt ampere unit size, in Japan since 1996 and Germany sinc

  4. Variable speed wind turbine control system

    Science.gov (United States)

    Conley, E.

    Variable speed wind turbine operation offers potential increased energy production if the turbine rotor is controlled to operate at constant blade tip speed to wind speed ratio. Two variable speed control systems are compared to a constant speed control system during field tests of a 5m Darrieus type wind turbine generator. Data indicates that a simple variable speed control scheme using wind rotor RPM as the single input signal can control the Darrieus test machine to operate at roughly constant blade tip to wind speed ratio and thus maximize energy production.

  5. Variable Speed Rotor System Project

    Data.gov (United States)

    National Aeronautics and Space Administration — Variable speed rotors will give helicopters several advantages: higher top speed, greater fuel efficiency, momentary emergency over-power, resonance detuning...

  6. Speed control variable rate irrigation

    Science.gov (United States)

    Speed control variable rate irrigation (VRI) is used to address within field variability by controlling a moving sprinkler’s travel speed to vary the application depth. Changes in speed are commonly practiced over areas that slope, pond or where soil texture is predominantly different. Dynamic presc...

  7. Increased speed and image quality in single-shot fast spin echo imaging via variable refocusing flip angles.

    Science.gov (United States)

    Loening, Andreas M; Saranathan, Manojkumar; Ruangwattanapaisarn, Nichanan; Litwiller, Daniel V; Shimakawa, Ann; Vasanawala, Shreyas S

    2015-12-01

    To develop and validate clinically a single-shot fast spin echo (SSFSE) sequence utilizing variable flip angle refocusing pulses to shorten acquisition times via reductions in specific absorption rate (SAR) and improve image quality. A variable refocusing flip angle SSFSE sequence (vrfSSFSE) was designed and implemented, with simulations and volunteer scans performed to determine suitable flip angle modulation parameters. With Institutional Review Board (IRB) approval/informed consent, patients referred for 3T abdominal magnetic resonance imaging (MRI) were scanned with conventional SSFSE and either half-Fourier (n = 25) or full-Fourier vrfSSFSE (n = 50). Two blinded radiologists semiquantitatively scored images on a scale from -2 to 2 for contrast, noise, sharpness, artifacts, cardiac motion-related signal loss, and the ability to evaluate the pancreas and kidneys. vrfSSFSE demonstrated significantly increased speed (∼2-fold, P parameters with full-Fourier vrfSSFSE included increased contrast, sharpness, and visualization of pancreatic and renal structures with higher bandwidth technique (mean scores 0.37, 0.83, 0.62, and 0.31, respectively, P ≤ 0.001), and decreased image noise and improved visualization of renal structures when used with an equal bandwidth technique (mean scores 0.96 and 0.35, respectively, P < 0.001). Increased cardiac motion-related signal loss with full-Fourier vrfSSFSE was seen in the pancreas but not the kidney. vrfSSFSE increases speed at 3T over conventional SSFSE via reduced SAR, and when combined with full-Fourier acquisition can improve image quality, although with some increased sensitivity to cardiac motion-related signal loss. © 2015 Wiley Periodicals, Inc.

  8. Motor Integrated Variable Speed Drives

    OpenAIRE

    Singh, Yash Veer

    2015-01-01

    A new trend in the variable speed drives (VSDs) is to develop fully integrated systems, which lead to low-cost products with shorter design cycles. Motor Integrated design of VSDs will reduce cable length to connect drive with machine windings and installation time for end user. The electric drives are expected to have minimum effect on grid and motor connected to it, i.e. currents drawn from grid should be within specified limits and currents injecting in to machine should not overheat the m...

  9. Motor Integrated Variable Speed Drives

    DEFF Research Database (Denmark)

    Singh, Yash Veer

    A new trend in the variable speed drives (VSDs) is to develop fully integrated systems, which lead to low-cost products with shorter design cycles. Motor Integrated design of VSDs will reduce cable length to connect drive with machine windings and installation time for end user. The electric drives...... are expected to have minimum effect on grid and motor connected to it, i.e. currents drawn from grid should be within specified limits and currents injecting in to machine should not overheat the machine windings to avoid insulation failure due to harmonics. It is also necessary that electric drives should...... when it comes to the development of any kind of power converter topology for power electronic applications. Concerning the use of a power converter in motor integrated VSDs, the first two mentioned aspects receive an even greater im-portance. Power converter design for integrated drives poses a host...

  10. Using Variable Speed Control on Pump Application

    Directory of Open Access Journals (Sweden)

    Dr.Sc. Aida Spahiu

    2012-06-01

    Full Text Available Pumps are one of the most common variable speed drive (VSD system applications and special interest has focused on improving their energy efficiency by using variable speed control instead of throttling or other less efficient flow control methods. Pumps are the single largest user of electricity in industry in the European Union, consuming 160 TWh per annum of electricity and accounting for 79 million tonnes of carbon dioxide (CO2 emissions [1]. Centrifugal pumps are the most likely pump style to provide a favorable return based on energy savings when applied with a variable speed drive. To help illustrate this, are conducted benchmark testing to document various head and flow scenarios and their corresponding effect on energy savings. Paper shows the relationship of static and friction head in the energy efficiency equation and the effect of motor, pump and VSD efficiencies. The received results are good reference points for engineers and managers of water sector in Albania to select the best prospects for maximizing efficiency and energy savings.

  11. Variable-speed, portable routing skate

    Science.gov (United States)

    Pesch, W. A.

    1967-01-01

    Lightweight, portable, variable-speed routing skate is used on heavy metal subassemblies which are impractical to move to a stationary machine. The assembly, consisting of the housing with rollers, router, and driving mechanism with transmission, weighs about forty pounds. Both speed and depth of cut are adjustable.

  12. Telegrapher's equation with variable propagation speeds

    OpenAIRE

    Masoliver, Jaume, 1951-; Weiss, George H. (George Herbert), 1930-

    1994-01-01

    All derivations of the one-dimensional telegraphers equation, based on the persistent random walk model, assume a constant speed of signal propagation. We generalize here the model to allow for a variable propagation speed and study several limiting cases in detail. We also show the connections of this model with anomalous diffusion behavior and with inertial dichotomous processes.

  13. About Variable Speed Heating and Cooling Pumps

    Directory of Open Access Journals (Sweden)

    Cătălin Popovici

    2009-01-01

    Full Text Available The present work has the purpose of underlying the advantages of variable speed heating and cooling pumps use for the perspective of general and particular pumping costs and efficiency. The study approaches comparisons between constant flow pumps and variable flow pumps in different given situations and comparatively analyses the pumping costs.

  14. About Variable Speed Heating and Cooling Pumps

    OpenAIRE

    Popovici, Cătălin; Ignat, Jan

    2009-01-01

    The present work has the purpose of underlying the advantages of variable speed heating and cooling pumps use for the perspective of general and particular pumping costs and efficiency. The study approaches comparisons between constant flow pumps and variable flow pumps in different given situations and comparatively analyses the pumping costs.

  15. Handbook of asynchronous machines with variable speed

    CERN Document Server

    Razik, Hubert

    2013-01-01

    This handbook deals with the asynchronous machine in its close environment. It was born from a reflection on this electromagnetic converter whose integration in industrial environments takes a wide part. Previously this type of motor operated at fixed speed, from now on it has been integrated more and more in processes at variable speed. For this reason it seemed useful, or necessary, to write a handbook on the various aspects from the motor in itself, via the control and while finishing by the diagnosis aspect. Indeed, an asynchronous motor is used nowadays in industry where variation speed a

  16. Miniature, Variable-Speed Control Moment Gyroscope

    Science.gov (United States)

    Bilski, Steve; Kline-Schoder, Robert; Sorensen, Paul

    2011-01-01

    The Miniature Variable-Speed Control Moment Gyroscope (MVS-CMG) was designed for small satellites (mass from less than 1 kg up to 500 kg). Currently available CMGs are too large and heavy, and available miniature CMGs do not provide sufficient control authority for use on practical satellites. This primarily results from the need to greatly increase the speed of rotation of the flywheel in order to reduce the flywheel size and mass. This goal was achieved by making use of a proprietary, space-qualified, high-speed (100,000 rpm) motor technology to spin the flywheel at a speed ten times faster than other known miniature CMGs under development. NASA is supporting innovations in propulsion, power, and guidance and navigation systems for low-cost small spacecraft. One of the key enabling technologies is attitude control mechanisms. CMGs are particularly attractive for spacecraft attitude control since they can achieve higher torques with lower mass and power than reaction wheels, and they provide continuous torque capability that enables precision pointing (in contrast to on-off thruster control). The aim of this work was to develop a miniature, variable-speed CMG that is sized for use on small satellites. To achieve improved agility, these spacecraft must be able to slew at high rate, which requires attitude control actuators that can apply torques on the order of 5 N-m. The MVS-CMG is specifically designed to achieve a high-torque output with a minimum flywheel and system mass. The flywheel can be run over a wide range of speeds, which is important to help reduce/eliminate potential gimbal lock, and can be used to optimize the operational envelope of the CMG.

  17. Virtual inertia for variable speed wind turbines

    DEFF Research Database (Denmark)

    Zeni, Lorenzo; Rudolph, Andreas Jakob; Münster-Swendsen, Janus

    2013-01-01

    Inertia provision for frequency control is among the ancillary services that different national grid codes will likely require to be provided by future wind turbines. The aim of this paper is analysing how the inertia response support from a variable speed wind turbine (VSWT) to the primary...... frequency control of a power system can be enhanced. Unlike fixed speed wind turbines, VSWTs do not inherently contribute to system inertia, as they are decoupled from the power system through electronic converters. Emphasis in this paper is on how to emulate VSWTs inertia using control of the power...... power with wind is also assessed and investigated. The intrinsic problems related to the implementation of virtual inertia are illustrated, addressing their origin in the action of pitch and power control. A solution is proposed, which aims at obtaining the same response as for the system with only...

  18. Control of Variable Speed Variable Pitch Wind Turbine at Above and Below Rated Wind Speed

    Directory of Open Access Journals (Sweden)

    Saravanakumar Rajendran

    2014-01-01

    Full Text Available The paper presents a nonlinear approach to wind turbine (WT using two-mass model. The main aim of the controller in the WT is to maximize the energy output at varying wind speed. In this work, a combination of linear and nonlinear controllers is adapted to variable speed variable pitch wind turbines (VSVPWT system. The major operating regions of the WT are below (region 2 and above rated (region 3 wind speed. In these regions, generator torque control (region 2 and pitch control (region 3 are used. The controllers in WT are tested for below and above rated wind speed for step and vertical wind speed profile. The performances of the controllers are analyzed with nonlinear FAST (Fatigue, Aerodynamics, Structures, and Turbulence WT dynamic simulation. In this paper, two nonlinear controllers, that is, sliding mode control (SMC and integral sliding mode control (ISMC, have been applied for region 2, whereas for pitch control in region 3 conventional PI control is used. In ISMC, the sliding manifold makes use of an integral action to show effective qualities of control in terms of the control level reduction and sliding mode switching control minimization.

  19. DESIGN METHODOLOGY FOR LOW SPEED VARIABLE RELUCTANCE MOTORS

    OpenAIRE

    Suriano, John R.

    1992-01-01

    Lowering the gear reduction in actuators by utilizing high-torque low-speed motors enables the use of less expensive and simpler gear systems and decreases the overall system inertia. Variable reluctance machines can produce high torque at low speeds. Their static torque, a critical quantity for determination of low speed operation, is colnpared for three variable reluctance motor design variations using linear analysis. Saturation effects, which are crucial to the accurate determination of s...

  20. Homogenization Effects of Variable Speed Limits

    National Research Council Canada - National Science Library

    Alvaro Garcia-Castro; Andres Monzon

    2014-01-01

    ... and their results This study also presents a key indicator which measures the speed homogeneity and a methodology to obtain the data based on floating cars and GPS technology applying it to a case study on a section of the M30 urban motorway in Madrid (Spain). It also presents the relation between this indicator and road performance and emissions values.

  1. Investigation of load reduction for a variable speed, variable pitch, and variable coning wind turbine

    Energy Technology Data Exchange (ETDEWEB)

    Pierce, K. [Univ. of Utah, Salt Lake City, UT (United States)

    1997-12-31

    A two bladed, variable speed and variable pitch wind turbine was modeled using ADAMS{reg_sign} to evaluate load reduction abilities of a variable coning configuration as compared to a teetered rotor, and also to evaluate control methods. The basic dynamic behavior of the variable coning turbine was investigated and compared to the teetered rotor under constant wind conditions as well as turbulent wind conditions. Results indicate the variable coning rotor has larger flap oscillation amplitudes and much lower root flap bending moments than the teetered rotor. Three methods of control were evaluated for turbulent wind simulations. These were a standard IPD control method, a generalized predictive control method, and a bias estimate control method. Each control method was evaluated for both the variable coning configuration and the teetered configuration. The ability of the different control methods to maintain the rotor speed near the desired set point is evaluated from the RMS error of rotor speed. The activity of the control system is evaluated from cycles per second of the blade pitch angle. All three of the methods were found to produce similar results for the variable coning rotor and the teetered rotor, as well as similar results to each other.

  2. Temperature variability, intensity of wind speed and visibility during ...

    African Journals Online (AJOL)

    The study assessed the connections between temperature variability, intensity of wind speed and their effect on visibility of Makurdi town during the harmattan season. Data on mean monthly temperature of harmattan months of November, December, January and February (2001 to 2011), Wind speed and visibility records ...

  3. Miniature, Variable-Speed Control Moment Gyroscope Project

    Data.gov (United States)

    National Aeronautics and Space Administration — The overall goal of this project is to design, develop, demonstrate, and deliver a miniature, variable speed control moment gyroscope (MVS CMG) for use on small...

  4. Variable speed generator technology options for wind turbine generators

    Science.gov (United States)

    Lipo, T. A.

    1995-01-01

    The electrical system options for variable speed operation of a wind turbine generator are treated in this paper. The key operating characteristics of each system are discussed and the major advantages and disadvantages of each are identified

  5. Critical Speed Control for a Fixed Blade Variable Speed Wind Turbine

    Directory of Open Access Journals (Sweden)

    Morgan Rossander

    2017-10-01

    Full Text Available A critical speed controller for avoiding a certain rotational speed is presented. The controller is useful for variable speed wind turbines with a natural frequency in the operating range. The controller has been simulated, implemented and tested on an open site 12 kW vertical axis wind turbine prototype. The controller is based on an adaptation of the optimum torque control. Two lookup tables and a simple state machine provide the control logic of the controller. The controller requires low computational resources, and no wind speed measurement is needed. The results suggest that the controller is a feasible method for critical speed control. The skipping behavior can be adjusted using only two parameters. While tested on a vertical axis wind turbine, it may be used on any variable speed turbine with the control of generator power.

  6. Small variable speed hermetic reciprocating compressors for domestic refrigerators

    DEFF Research Database (Denmark)

    Rasmussen, Bjarne D.

    1996-01-01

    This paper contains both a theoretical and experimental investigation of some of the fundamental characteristics of a smal variable speed hermetic reciprocating compressor intended for application in domestic refrigeration. The results of a previously published simulation model for variable speed...... compressors are compared with experimental results obtained in a compressor test bench. The influence of speed on compressor performance is discussed with focus on valve modelling and internal thermal phenomena. Further plans for development and validation of the model as well as experimental investigations...

  7. Pitch Angle Control for Variable Speed Wind Turbines

    DEFF Research Database (Denmark)

    Chen, Zhe; Zhang, Jianzhong; Cheng, M

    2008-01-01

    Pitch angle control is the most common means for adjusting the aerodynamic torque of the wind turbine when wind speed is above rated speed and various controlling variables may be chosen, such as wind speed, generator speed and generator power. As conventional pitch control usually use PI...... controller, the mathematical model of the system should be known well. A fuzzy logic pitch angle controller is developed in this paper, in which it does not need well known about the system and the mean wind speed is used to compensate the non-linear sensitivity. The fuzzy logic control strategy may have...... the potential when the system contains strong non-linearity, such as wind turbulence is strong, or the control objectives include fatigue loads. The design of the fuzzy logic controller and the comparisons with conversional pitch angle control strategies with various controlling variables are carried out...

  8. Variable speed drives principles and applications for energy cost savings

    CERN Document Server

    Spitzer, David William

    2014-01-01

    Simply put, a variable speed drive is a controller that allows a motor and its associated equipment to run at different speeds depending upon automated input from an industrial process. That in turn provides the ability to provide smoother operations, and most importantly, energy savings by slowing down machinery when a process does not have to run at full speed. Long a leading book on this technology, this new edition by industry authority David William Spitzer provides insights to improving the applications of variable speed drives. Whether you have basic knowledge or advanced knowledge, you will find this book to be an extremely useful introduction to how variable speed drivers work, how they are best applied, and what to do and what to avoid when employing them as part of an overall automated industrial enterprise, all with an eye on energy savings. Inside, you will find: A basic overview of electrical, hydraulic, and instrumentation principles of variable speed drives. Coverage of the role that variabl...

  9. Survey of variable speed operation of wind turbines

    Energy Technology Data Exchange (ETDEWEB)

    Carlson, Ola; Hylander, J.; Thorborg, K. [Chalmers Univ. of Technology, Goeteborg (Sweden). Dept. of Electric Power Engineering

    1996-12-01

    During the last five years the production and operation of variable-speed wind turbines have advanced from a few experimental machines to a serial production of at least 10 MW of installed capacity of variable speed machines per week. The rated power of serial wind turbines is today around 600 kW and for the prototypes up to 3000 kW. Variable speed operation of wind turbines can be obtained with several different types of electrical generating systems, such as synchronous generators with diode rectifiers and thyristor inverters or induction generators with IGBT-converters, for the wide speed range. For the narrow speed range the wound motor induction generator with a rotor cascade or a controlled rotor resistance is preferable. The development of permanent magnetic material and the reduction of costs of the power electronic components have opened a possibility of designing cost-effective wind turbines with a directly driven generator. Pitch control together with variable speed will make it possible to limit the power variation within a few percent, 2 to 5 %, of the rated power. 7 refs, 4 figs, 2 tabs

  10. Improving transition between power optimization and power limitation of variable speed/variable pitch wind turbines

    Energy Technology Data Exchange (ETDEWEB)

    Hansen, A.D.; Bindner, H. [Risoe National Lab., Wind Energy and Atmospheric Physics Dept., Roskilde (Denmark); Rebsdorf, A. [Vestas Wind Systems A/S, Lem (Denmark)

    1999-03-01

    The paper summarises and describes the main results of a recently performed study of improving the transition between power optimization and power limitation for variable speed/variable pitch wind turbines. The results show that the capability of varying the generator speed also can be exploited in the transition stage to improve the quality of the generated power. (au)

  11. Application of variable speed operation on Francis Turbines

    Directory of Open Access Journals (Sweden)

    G. P. Heckelsmueller

    2015-04-01

    Full Text Available Francis turbines that are directly coupled to a synchronous generator operate at constant rotational speed around a design point characterized by a given water head, flow and guide vane aperture. When important changes occur in headwater level in power stations with large reservoirs, the turbines suffer a significant loss of efficiency. By applying variable speed technology it may be possible to adapt the runner speed and to operate with a higher efficiency over a wide range of water heads. This investigation is intended to reveal the possible benefits of using variable speed operation in regard to gains in efficiency and power output. Based on model test data it is possible to determine the characteristic curves of unitary speed and unitary flow of the respective prototype turbine for varying guide vane apertures. By varying rotor speed it is possible to maintain values that correspond to maximum efficiency. An analysis is made keeping guide vane aperture constant and introducing a proportionality factor of water flow to corresponding power output. The results show that for guide vane apertures and heads different from the design point, best efficiencies can be kept by adjusting rotor speed. At heads lower than the design head, significant efficiency gains can be achieved. Consequently, a significant proportion of the flow can be saved while generating the same amount of power.

  12. Flicker Mitigation by Speed Control of Permanent Magnet Synchronous Generator Variable-Speed Wind Turbines

    DEFF Research Database (Denmark)

    Hu, Weihao; Zhang, Yunqian; Chen, Zhe

    2013-01-01

    Grid-connected wind turbines are fluctuating power sources that may produce flicker during continuous operation. This paper presents a simulation model of a MW-level variable speed wind turbine with a full-scale back-to-back power converter and permanent magnet synchronous generator (PMSG......) developed in the simulation tool of PSCAD/EMTDC. Flicker emission of this system is investigated. The 3p (three times per revolution) power oscillation due to wind shear and tower shadow effects is the significant part in the flicker emission of variable speed wind turbines with PMSG during continuous...... operation. A new method of flicker mitigation by controlling the rotational speed is proposed. It smoothes the 3p active power oscillations from wind shear and tower shadow effects of the wind turbine by varying the rotational speed of the PMSG. Simulation results show that damping the 3p active power...

  13. Flicker Mitigation by Speed Control of Permanent Magnet Synchronous Generator Variable-Speed Wind Turbines

    Directory of Open Access Journals (Sweden)

    Yanting Hu

    2013-07-01

    Full Text Available Grid-connected wind turbines are fluctuating power sources that may produce flicker during continuous operation. This paper presents a simulation model of a MW-level variable speed wind turbine with a full-scale back-to-back power converter and permanent magnet synchronous generator (PMSG developed in the simulation tool of PSCAD/EMTDC. Flicker emission of this system is investigated. The 3p (three times per revolution power oscillation due to wind shear and tower shadow effects is the significant part in the flicker emission of variable speed wind turbines with PMSG during continuous operation. A new method of flicker mitigation by controlling the rotational speed is proposed. It smoothes the 3p active power oscillations from wind shear and tower shadow effects of the wind turbine by varying the rotational speed of the PMSG. Simulation results show that damping the 3p active power oscillation by using the flicker mitigation speed controller is an effective means for flicker mitigation of variable speed wind turbines with full-scale back-to-back power converters and PMSG during continuous operation.

  14. Review of Test Procedure for Determining HSPFs of Residential Variable-Speed Heat Pumps

    Energy Technology Data Exchange (ETDEWEB)

    Rice, C. Keith [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Munk, Jeffrey D. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Shrestha, Som S. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

    2015-08-01

    This report reviews the suitability of the existing Heating Seasonal Performance Factor (HSPF) ratings and testing requirements for the current generation of variable-speed (VS) air-source heat pumps. Recent field test results indicate larger discrepancies between rated HSPF and field-observed HSPF for VS models than for single-speed models in the same houses. These findings suggest that the heating season test and ratings procedure should be revisited for VS heat pumps. The ratings and testing procedures are described in ANSI/AHRI 210/240 (2008) for single-speed, two-capacity, and variable-speed units. Analysis of manufacturer and independent test performance data on VS units reveals why the current VS testing/ratings procedure results in overly optimistic HSPF ratings for some VS units relative to other types of heat pumps. This is due to a combination of extrapolation of low speed test data beyond the originally anticipated ambient temperature operating range and the constraints of unit controls, which prevent low speed operation over the range of ambient temperatures assumed in the procedure for low speed. As a result, the HSPFs of such units are being overpredicted relative to those for single- and two-capacity designs. This overprediction has been found to be significantly reduced by use in the HSPF ratings procedure of an alternative higher-load heating load line, described in a companion report (Rice et al., 2015).

  15. Implementation of Temperature Sequential Controller on Variable Speed Drive

    Science.gov (United States)

    Cheong, Z. X.; Barsoum, N. N.

    2008-10-01

    There are many pump and motor installations with quite extensive speed variation, such as Sago conveyor, heating, ventilation and air conditioning (HVAC) and water pumping system. A common solution for these applications is to run several fixed speed motors in parallel, with flow control accomplish by turning the motors on and off. This type of control method causes high in-rush current, and adds a risk of damage caused by pressure transients. This paper explains the design and implementation of a temperature speed control system for use in industrial and commercial sectors. Advanced temperature speed control can be achieved by using ABB ACS800 variable speed drive-direct torque sequential control macro, programmable logic controller and temperature transmitter. The principle of direct torque sequential control macro (DTC-SC) is based on the control of torque and flux utilizing the stator flux field orientation over seven preset constant speed. As a result of continuous comparison of ambient temperature to the references temperatures; electromagnetic torque response is particularly fast to the motor state and it is able maintain constant speeds. Experimental tests have been carried out by using ABB ACS800-U1-0003-2, to validate the effectiveness and dynamic respond of ABB ACS800 against temperature variation, loads, and mechanical shocks.

  16. Active surge control for variable speed axial compressors.

    Science.gov (United States)

    Lin, Shu; Yang, Chunjie; Wu, Ping; Song, Zhihuan

    2014-09-01

    This paper discusses active surge control in variable speed axial compressors. A compression system equipped with a variable area throttle is investigated. Based on a given compressor model, a fuzzy logic controller is designed for surge control and a proportional speed controller is used for speed control. The fuzzy controller uses measurements of the change of pressure rise as well as the change of mass flow to determine the throttle opening. The presented approach does not require the knowledge of system equilibrium or the surge line. Numerical simulations show promising results. The proposed fuzzy logic controller performs better than a backstepping controller and is capable to suppress surge at different operating points. Copyright © 2014 ISA. Published by Elsevier Ltd. All rights reserved.

  17. Design Methodology for Low-Speed Variable Reluctance Motors

    Science.gov (United States)

    Suriano, John Riden

    Lowering the gear reduction in actuators by utilizing high-torque low-speed motors enables the use of less expensive and simpler gear systems and decreases the overall system inertia. Variable reluctance machines can produce high torque at low speeds. Their static torque, a critical quantity for determination of low speed operation, is compared for three variable reluctance motor design variations using linear analysis. Saturation effects, which are crucial to the accurate determination of static torque, are modeled using a dual energy technique first proposed by Lord Rayleigh. Dual energy techniques utilizing flux tubes and magnetomotive force slices are developed into a numerical method for predicting nonlinear three-dimensional magnetostatic field parameters. The dual energy method offers a compromise between the accurate but laborious finite element method and the speed of simplified lumped parameter magnetic circuit calculations. A two-dimensional dual energy model of a variable reluctance motor is developed. Results of calculations on a 4 kW Oulton machine are compared to measurements and other calculation methods. Finally, as a demonstration, the model is used to evaluate two competing variable reluctance motors for use as replacements for a DC windshield wiper motor.

  18. Considering Variable Road Geometry in Adaptive Vehicle Speed Control

    Directory of Open Access Journals (Sweden)

    Xinping Yan

    2013-01-01

    Full Text Available Adaptive vehicle speed control is critical for developing Advanced Driver Assistance Systems (ADAS. Vehicle speed control considering variable road geometry has become a hotspot in ADAS research. In this paper, first, an exploration of intrinsic relationship between vehicle operation and road geometry is made. Secondly, a collaborative vehicle coupling model, a road geometry model, and an AVSC, which can respond to variable road geometry in advance, are developed. Then, based on H∞ control method and the minimum energy principle, a performance index is specified by a cost function for the proposed AVSC, which can explicitly consider variable road geometry in its optimization process. The proposed AVSC is designed by the Hamilton-Jacobi Inequality (HJI. Finally, simulations are carried out by combining the vehicle model with the road geometry model, in an aim of minimizing the performance index of the AVSC. Analyses of the simulation results indicate that the proposed AVSC can automatically and effectively regulate speed according to variable road geometry. It is believed that the proposed AVSC can be used to improve the economy, comfort, and safety effects of current ADAS.

  19. Systematic Controller Design Methodology for Variable-Speed Wind Turbines

    Energy Technology Data Exchange (ETDEWEB)

    Hand, M. M.; Balas, M. J.

    2002-02-01

    Variable-speed, horizontal axis wind turbines use blade-pitch control to meet specified objectives for three operational regions. This paper provides a guide for controller design for the constant power production regime. A simple, rigid, non-linear turbine model was used to systematically perform trade-off studies between two performance metrics. Minimization of both the deviation of the rotor speed from the desired speed and the motion of the actuator is desired. The robust nature of the proportional-integral-derivative controller is illustrated, and optimal operating conditions are determined. Because numerous simulation runs may be completed in a short time, the relationship between the two opposing metrics is easily visualized.

  20. An Analysis of Variable-Speed Wind Turbine Power-Control Methods with Fluctuating Wind Speed

    Directory of Open Access Journals (Sweden)

    Seung-Il Moon

    2013-07-01

    Full Text Available Variable-speed wind turbines (VSWTs typically use a maximum power-point tracking (MPPT method to optimize wind-energy acquisition. MPPT can be implemented by regulating the rotor speed or by adjusting the active power. The former, termed speed-control mode (SCM, employs a speed controller to regulate the rotor, while the latter, termed power-control mode (PCM, uses an active power controller to optimize the power. They are fundamentally equivalent; however, since they use a different controller at the outer control loop of the machine-side converter (MSC controller, the time dependence of the control system differs depending on whether SCM or PCM is used. We have compared and analyzed the power quality and the power coefficient when these two different control modes were used in fluctuating wind speeds through computer simulations. The contrast between the two methods was larger when the wind-speed fluctuations were greater. Furthermore, we found that SCM was preferable to PCM in terms of the power coefficient, but PCM was superior in terms of power quality and system stability.

  1. Design Optimization of a Variable-Speed Power Turbine

    Science.gov (United States)

    Hendricks, Eric S.; Jones, Scott M.; Gray, Justin S.

    2014-01-01

    NASA's Rotary Wing Project is investigating technologies that will enable the development of revolutionary civil tilt rotor aircraft. Previous studies have shown that for large tilt rotor aircraft to be viable, the rotor speeds need to be slowed significantly during the cruise portion of the flight. This requirement to slow the rotors during cruise presents an interesting challenge to the propulsion system designer as efficient engine performance must be achieved at two drastically different operating conditions. One potential solution to this challenge is to use a transmission with multiple gear ratios and shift to the appropriate ratio during flight. This solution will require a large transmission that is likely to be maintenance intensive and will require a complex shifting procedure to maintain power to the rotors at all times. An alternative solution is to use a fixed gear ratio transmission and require the power turbine to operate efficiently over the entire speed range. This concept is referred to as a variable-speed power-turbine (VSPT) and is the focus of the current study. This paper explores the design of a variable speed power turbine for civil tilt rotor applications using design optimization techniques applied to NASA's new meanline tool, the Object-Oriented Turbomachinery Analysis Code (OTAC).

  2. Optimal Control Structure For Variable Speed Wind Power System

    Directory of Open Access Journals (Sweden)

    Emil CEANGA

    2002-12-01

    Full Text Available This paper presents an optimal control structure for variable speed, fixed pitch wind turbine. The control objective results from the optimization criterion that includes two contradictory demands: maximization of the energy captured from the wind and minimization of the damage caused by mechanical fatigue. We admit, as a modeling assumption, that wind speed has two components: a slowly varying component, named seasonal and a rapidly varying component, named turbulence. Hence, two control structures, which should function simultaneously, are identified in the optimal control problem.The first optimization structure aims to maximize the wind turbine energetic efficiency by maintaining operational point on optimal regime characteristics (ORC. According to the slowly varying seasonal component of wind speed, a control loop adjusts system operating point in order to maintain it on ORC. In the second optimization structure, the system is considered to be operating on “static” optimal point assured by the above mentioned control loop, with the turbulence component being the input variable. The second control loop aims dynamic optimization that implies the minimization of the tip speed ratio variations around its optimal value, while minimizing the torque variations, thus the mechanical stress. Mathematically, this objective is defined as an integral criterion, belonging to linear quadratic optimization.

  3. Dynamic Performance of a Single Machine Brushless DFIG during Wind Speed Variation

    OpenAIRE

    Mona N. Eskander; Saleh, Mahmoud A.; Maged N. F. Nashed

    2015-01-01

    In this paper, the dynamic performance of the specially-designed Single Machine Brushless Doubly-Fed Induction Generator "SM-BDFIG" coupled to variable speed wind turbine is investigated. The rotor voltage of the SM-BDFIG during super-synchronous operation is used to charge a number of batteries, connected to the rotor via a 3- phase bridge rectifier. The number of charged battery cells are changed, by parallel and series connections, according to the wind speed variat...

  4. Variable speed hermetic reciprocating compressors for domestic refrigerators

    DEFF Research Database (Denmark)

    Rasmussen, Bjarne D.

    1998-01-01

    This article describes the results of a both theoretical and experimental investigation of the performance of variable speed hermetic reciprocating compressors for domestic refrigerators. The investigation was performed as a part of a larger research project with the objective of reducing...... the energy consumption of domestic refrigerators by increasing the efficiency of the refrigeration system. The improvement of the system efficiency was to be obtained by introducing continuous operation and use variable speed compressors for controlling the capacity of the refrigeration system....... It was the aim of the project to double the efficiency of the refrigeration system and thereby reduce the energy consumption of the refrigerator by 50% compared to a standard refrigerator available on the market to day....

  5. Load flow analysis for variable speed offshore wind farms

    DEFF Research Database (Denmark)

    Chen, Zhe; Zhao, Menghua; Blaabjerg, Frede

    2009-01-01

    A serial AC-DC integrated load flow algorithm for variable speed offshore wind farms is proposed. It divides the electrical system of a wind farm into several local networks, and different load flow methods are used for these local networks sequentially. This method is fast, more accurate, and many...... factors such as the different wind farm configurations, the control of wind turbines and the power losses of pulse width modulation converters are considered. The DC/DC converter model is proposed and integrated into load flow algorithm by modifying the Jacobian matrix. Two iterative methods are proposed...... and integrated into the load flow algorithm: one takes into account the control strategy of converters and the other considers the power losses of converters. In addition, different types of variable speed wind turbine systems with different control methods are investigated. Finally, the method is demonstrated...

  6. Adaptive Torque Control of Variable Speed Wind Turbines

    Energy Technology Data Exchange (ETDEWEB)

    Johnson, K. E.

    2004-08-01

    The primary focus of this work is a new adaptive controller that is designed to resemble the standard non-adaptive controller used by the wind industry for variable speed wind turbines below rated power. This adaptive controller uses a simple, highly intuitive gain adaptation law designed to seek out the optimal gain for maximizing the turbine's energy capture. It is designed to work even in real, time-varying winds.

  7. Tip Speed Ratio Based Maximum Power Tracking Control of Variable Speed Wind Turbines; A Comprehensive Design

    Directory of Open Access Journals (Sweden)

    Murat Karabacak

    2017-08-01

    Full Text Available The most primitive control method of wind turbines used to generate electric energy from wind is the fixed speed control method. With this method, it is not possible that turbine input power is transferred to grid at maximum rate. For this reason, Maximum Power Tracking (MPT schemes are proposed. In order to implement MPT, the propeller has to rotate at a different speed for every different wind speed. This situation has led MPT based systems to be called Variable Speed Wind Turbine (VSWT systems. In VSWT systems, turbine input power can be transferred to grid at rates close to maximum power. When MPT based control of VSWT systems is the case, two important processes come into prominence. These are instantaneously determination and tracking of MPT point. In this study, using a Maximum Power Point Tracking (MPPT method based on tip speed ratio, power available in wind is transferred into grid over a back to back converter at maximum rate via a VSWT system with permanent magnet synchronous generator (PMSG. Besides a physical wind turbine simulator is modelled and simulated. Results show that a time varying MPPT point is tracked with a high performance.

  8. Variable speed rotary compressor and adjustable speed drive efficiencies measured in the laboratory

    Science.gov (United States)

    Miller, W. A.

    Two state-of-the-art variable-speed rotary compressors, of nominal one ton cooling capacity, were instrumented and tested in the laboratory. Both compressors were identical except for their respective variable-speed drive systems (i.e., motor and inverter). One compressor had an inverter driven induction motor (IDIM) drive, and the other had a permanent magnet electronically commutated motor drive (PM-ECM). The laboratory study evaluated the compressor's efficiency under representative variable-speed conditions. Testing was conducted as a function of compressor drive frequency and of refrigerant condensing and evaporating conditions. Saturated refrigerant conditions, inlet superheat, and subcooling were controlled using a secondary refrigerant calorimeter. Spectrum analysis was conducted on the current input to one phase of the three-phase drive systems to measure motor speed and characterize harmonic content of the inverters. An optimal volt per Hz ratio was determined at 120-, 90-, 60-, and 30-Hz drive frequencies and at different load conditions for the rotary with induction motor as driven by a PWM inverter and also by a motor generator set (ideal induction motor drive). Variation of voltage input to the compressor had the largest effect at the lowest drive frequency (30Hz). A 5 percent variation about the optimal voltage at 30 Hz frequency caused a roughly 5 percent drop in compressor isentropic efficiency. Calorimeter data were used to develop modulating compressor and drive system performance maps. Performances of the two compressors were compared and the rotary with PM-ECM drive showed better efficiency trends at 30-Hz drive frequency. Above the 30-Hz drive frequency no clear advantage was observed for the PM-ECM vs the IDIM, possibly due to oversizing of the PM-ECM inverter.

  9. Pitch link loads reduction of variable speed rotors by variable tuning frequency fluidlastic isolators

    Directory of Open Access Journals (Sweden)

    Han Dong

    2015-10-01

    Full Text Available To reduce the pitch link loads of variable speed rotors, variable tuning frequency fluidlastic isolators are proposed. This isolator utilizes the variation of centrifugal force due to the change of rotor speed to change the tuning port area ratio, which can change the tuning frequency of the isolator. A rotor model including the model of fluidlastic isolator is coupled with a fuselage model to predict the steady responses of the rotor system in forward flight. The aeroelastic analyses indicate that distinct performance improvement in pitch link load control can be achieved by the utilization of variable frequency isolators compared with the constant tuning frequency isolators. The 4/rev (per revolution pitch link load is observed to be reduced by 87.6% compared with the increase of 56.3% by the constant frequency isolator, when the rotor speed is reduced by 16.7%. The isolation ability at different rotor speeds in different flight states is investigated. To achieve overall load reduction within the whole range of rotor speed, the strategy of the variation of tuning frequency is adjusted. The results indicate that the 4/rev pitch link load within the whole rotor speed range is decreased.

  10. LQG Controller Design for Pitch Regulated Variable Speed Wind Turbine

    DEFF Research Database (Denmark)

    Imran, Raja Muhammed; Hussain, Dil Muhammad Akbar; Chen, Zhe

    2014-01-01

    the effect of disturbance acting on its rotor blades by wind. CART2 (Control Advanced Research Turbine) linear model is produced/generated by FAST (Fatigue, Aerodynamics, Structures and Turbulence) code to test its simulation on MATLAB/Simulink and various results are compared. The designed controller......Variable speed wind turbine is a complex and nonlinear system, a sophisticated control is required to meet the challenges posed by these systems. This paper is presenting a pitch regulation strategy based on LQG (Linear Quadratic Gaussian) to regulate turbine at its rated power and to reject...

  11. Grid impact of variable-speed wind turbines

    Energy Technology Data Exchange (ETDEWEB)

    Larsson, Aa. [Chalmers Univ. of Technology, Dept. of Electric Power Engineering, Goeteborg (Sweden); Soerensen, P. [Risoe National Lab., Roskilde (Denmark); Santjer, F. [German Wind Energy Inst., DEWI, Wilhelmshaven (Germany)

    1999-03-01

    In this paper the power quality of variable-speed wind turbines equipped with forced-commutated inverters is investigated. Measurements have been taken on the same type of variable-speed wind turbines in Germany and Sweden. The measurements have been analysed according to existing IEC standards. Special attention has been paid to the aggregation of several wind turbines on flicker emission and harmonics. The aggregation has been compared with the summation laws used in the draft IEC 61400-21 `Power Quality Requirements for Grid Connected wind turbines`. The methods for calculating and summing flicker proposed by IEC Standards are reliable. Harmonics and inter-harmonics are treated in IEC 61000-4-7 and IEC 61000-3-6. The methods for summing harmonics and inter-harmonics in IEC 61000-3-6 are applicable to wind turbines. In order to obtain a correct magnitude of the frequency components, the use of a well-defined window width, according to IEC 61000-4-7 Amendment 1 is of a great importance. (au)

  12. Control of variable speed variable pitch wind turbine based on a disturbance observer

    Science.gov (United States)

    Ren, Haijun; Lei, Xin

    2017-11-01

    In this paper, a novel sliding mode controller based on disturbance observer (DOB) to optimize the efficiency of variable speed variable pitch (VSVP) wind turbine is developed and analyzed. Due to the highly nonlinearity of the VSVP system, the model is linearly processed to obtain the state space model of the system. Then, a conventional sliding mode controller is designed and a DOB is added to estimate wind speed. The proposed control strategy can successfully deal with the random nature of wind speed, the nonlinearity of VSVP system, the uncertainty of parameters and external disturbance. Via adding the observer to the sliding mode controller, it can greatly reduce the chattering produced by the sliding mode switching gain. The simulation results show that the proposed control system has the effectiveness and robustness.

  13. Comparison between OpenFOAM CFD & BEM theory for variable speedvariable pitch HAWT

    Directory of Open Access Journals (Sweden)

    ElQatary Islam

    2014-01-01

    Full Text Available OpenFoam is used to compare computational fluid dynamics (CFD with blade element momentum theory (BEM for a variable speed - variable pitch HAWT (Horizontal Axis Wind Turbine. The wind turbine is first designed using the BEM to determine the blade chord, twist and operating conditions. The wind turbine blade has an outer diameter of 14 m, uses a NACA 63–415 profile for the entire blade and root to tip twist distribution of 15deg (Figure 3. The RPM varies from 20–75 for freestream velocities varying between 3–10.5 m/s (variable speed and a constant RPM of 78.78 for velocities ranging between 11–25 m/s (variable pitch. OpenFOAM is used to investigate the wind turbine performance at several operating points including cut-in wind speed (3 m/s, rated wind speed (10.5 m/s and in the variable pitch zone. Simulation results show that in the variable-speed operating range, both CFD and BEM compare reasonably well. This agreement can be attributed to the fact that the complex three-dimensional flow around the turbine blades can be split into two radial segments. For radii less than the mid-span, the flow is three-dimensional, whereas for radii greater than the mid-span, the flow is approximately two-dimensional. Since the majority of the power is produced from sections beyond the mid-span, the agreement between CFD and BEM is reasonable. For the variable-pitch operating range the CFD results and BEM deviate considerably. In this case the majority of the power is produced from the inner sections in which the flow is three-dimensional and can no longer be predicted by the BEM. The results show that differences in pitch angles up to 10deg can result to regulate the power for high wind speeds in the variable-pitch operation zone.

  14. Fuzzy logic based variable speed wind generation system

    Energy Technology Data Exchange (ETDEWEB)

    Simoes, M.G. [Sao Paulo Univ., SP (Brazil). Escola Politecnica. PMC - Mecatronica; Bose, B.K. [Tennessee Univ., Knoxville, TN (United States). Dept. of Electrical Engineering; Spiegel, Ronal J. [Environmental Protection Agency, Research Triangle Park, NC (United States). Air and Energy Engineering Research Lab.

    1996-12-31

    This work demonstrates the successful application of fuzzy logic to enhance the performance and control of a variable speed wind generation system. A maximum power point tracker control is performed with three fuzzy controllers, without wind velocity measurement, and robust to wind vortex and turbine torque ripple. A squirrel cage induction generator feeds the power to a double-sided PWM converter system which pumps the power to a utility grid or supplies to an autonomous system. The fuzzy logic controller FLC-1 searches on-line the generator speed so that the aerodynamic efficiency of the wind turbine is optimized. A second fuzzy controller FLC-2 programs the machine flux by on-line search so as to optimize the machine-converter system wind vortex. Detailed analysis and simulation studies were performed for development of the control strategy and fuzzy algorithms, and a DSP TMS320C30 based hardware with C control software was built for the performance evaluation of a laboratory experimental set-up. The theoretical development was fully validated and the system is ready to be reproduced in a higher power installation. (author) 7 refs., 3 figs., 1 tab.

  15. Smart Algorithms to Control a Variable Speed Wind Turbine

    Directory of Open Access Journals (Sweden)

    Nabil Farhane

    2017-12-01

    Full Text Available In this paper, a robust adaptive fuzzy neural network sliding mode (AFNNSM control design is proposed to maximize the captured energy for a variable speed wind turbine and to minimize the efforts of the drive shaft. Fuzzy neural network (FNN is used to improve the mathematical system model, by the prediction of model unknown function, which is used by the Sliding mode control approach (SMC and enables a lower switching gain to be used despite the presence of large uncertainties. As a result, the used robust control action did not exhibit any chattering behavior. This FNN is trained on-line using the backpropagation algorithm (BP. The particle swarm optimization (PSO algorithm is used in this study to optimize the learning rate of BP algorithm in order to improve the network performance in term of the speed of convergence. The stability is shown by the Lyapunov theory and the trajectory tracking errors converge to zero without any oscillatory behavior. Simulations illustrate the effectiveness of the designed method.

  16. Variable-Speed, Robust Synchronous Reluctance Machine Drive Systems

    DEFF Research Database (Denmark)

    Wang, Dong

    premium efficiency machine and commercial products are available in the market. The research work in this dissertation aims at developing a simple, compact and robust synchronous reluctance machine drive system that can provide satisfactory performance with optimized system energy efficiency at various...... cooperate with the sensorless control well. Thus, the flux linkage based position sensorless control with adaptive PI controllers and “virtual positive impedance” active damping technique is recommended as the solution of the simple and robust variable-speed syn-chronous reluctance machine drive system.......The synchronous reluctance machine drive is getting more and more interests from the industrial side, since it can provide higher system energy efficiency than traditional inverter-fed induction machine drive systems with similar production cost. It is considered as a good candidate for super...

  17. Computer Aided Design Of Electrical Machines For Variable Speed Applications.

    Science.gov (United States)

    Krishnan, R.; Aravind, S.; Materu, P.

    1987-10-01

    In recent years, the product life cycle has decreased and demands for new products have emerged due to competition, modern industrial needs and rapidly changing technology. This has necessitated changes in design, development and manufacturing processes so as to improve quality and efficiency as well as reducing costs. Computer Aided Design (CAD) helps to meet this challenge in the design evaluation and final product design stages. This paper presents the development of an interactive software for the optimal design of a motor intended for variable speed applications. The use of finite element analysis methods is proposed as an indispensable part of the CAD system for electrical machine design. An illustration of the method is given for the design of a switched reluctance motor.

  18. Rasch's model for reading speed with manifest explanatory variables

    NARCIS (Netherlands)

    Jansen, G.G.H.

    In educational and psychological measurement we find the distinction between speed and power tests. Although most tests are partially speeded, the speed element is usually neglected. Here we consider a latent trait model developed by Rasch for the response time on a (set of) pure speed test(s),

  19. Preliminary Assessment of Variable Speed Power Turbine Technology on Civil Tiltrotor Size and Performance

    Science.gov (United States)

    Snyder, Christopher A.; Acree, Cecil W., Jr.

    2012-01-01

    A Large Civil Tiltrotor (LCTR) conceptual design was developed as part of the NASA Heavy Lift Rotorcraft Systems Investigation in order to establish a consistent basis for evaluating the benefits of advanced technology for large tiltrotors. The concept has since evolved into the second-generation LCTR2, designed to carry 90 passengers for 1,000 nm at 300 knots, with vertical takeoff and landing capability. This paper performs a preliminary assessment of variable-speed power turbine technology on LCTR2 sizing, while maintaining the same, advanced technology engine core. Six concepts were studied; an advanced, single-speed engine with a conventional power turbine layout (Advanced Conventional Engine, or ACE) using a multi-speed (shifting) gearbox. There were five variable-speed power turbine (VSPT) engine concepts, comprising a matrix of either three or four turbine stages, and fixed or variable guide vanes; plus a minimum weight, twostage, fixed-geometry VSPT. The ACE is the lightest engine, but requires a multi-speed (shifting) gearbox to maximize its fuel efficiency, whereas the VSPT concepts use a lighter, fixed-ratio gearbox. The NASA Design and Analysis of Rotorcraft (NDARC) design code was used to study the trades between rotor and engine efficiency and weight. Rotor performance was determined by Comprehensive Analytical Model of Rotorcraft Aerodynamics and Dynamics (CAMRAD II), and engine performance was estimated with the Numerical Propulsion System Simulation (NPSS). Design trades for the ACE vs. VSPT are presented in terms of vehicle gross and empty weight, propulsion system weight and mission fuel burn for the civil mission. Because of its strong effect on gearbox weight and on both rotor and engine efficiency, rotor speed was chosen as the reference design variable for comparing design trades. Major study assumptions are presented and discussed. Impressive engine power-to-weight and fuel efficiency reduced vehicle sensitivity to propulsion system choice

  20. High-speed single-pixel digital holography

    Science.gov (United States)

    González, Humberto; Martínez-León, Lluís.; Soldevila, Fernando; Araiza-Esquivel, Ma.; Tajahuerce, Enrique; Lancis, Jesús

    2017-06-01

    The complete phase and amplitude information of biological specimens can be easily determined by phase-shifting digital holography. Spatial light modulators (SLMs) based on liquid crystal technology, with a frame-rate around 60 Hz, have been employed in digital holography. In contrast, digital micro-mirror devices (DMDs) can reach frame rates up to 22 kHz. A method proposed by Lee to design computer generated holograms (CGHs) permits the use of such binary amplitude modulators as phase-modulation devices. Single-pixel imaging techniques record images by sampling the object with a sequence of micro-structured light patterns and using a simple photodetector. Our group has reported some approaches combining single-pixel imaging and phase-shifting digital holography. In this communication, we review these techniques and present the possibility of a high-speed single-pixel phase-shifting digital holography system with phase-encoded illumination. This system is based on a Mach-Zehnder interferometer, with a DMD acting as the modulator for projecting the sampling patterns on the object and also being used for phase-shifting. The proposed sampling functions are phaseencoded Hadamard patterns generated through a Lee hologram approach. The method allows the recording of the complex amplitude distribution of an object at high speed on account of the high frame rates of the DMD. Reconstruction may take just a few seconds. Besides, the optical setup is envisaged as a true adaptive system, which is able to measure the aberration induced by the optical system in the absence of a sample object, and then to compensate the wavefront in the phasemodulation stage.

  1. AC-DC integrated load flow calculation for variable speed offshore wind farms

    DEFF Research Database (Denmark)

    Zhao, Menghua; Chen, Zhe; Blaabjerg, Frede

    2005-01-01

    This paper proposes a sequential AC-DC integrated load flow algorithm for variable speed offshore wind farms. In this algorithm, the variable frequency and the control strategy of variable speed wind turbine systems are considered. In addition, the losses of wind turbine systems and the losses...... of converters are also integrated into the load flow algorithm. As a general algorithm, it can be applied to different types of wind farm configurations, and the load flow is related to the wind speed....

  2. Regulation of variable speed pumps in air conditioning; Regulation des pompes a vitesse variable en climatisation

    Energy Technology Data Exchange (ETDEWEB)

    Crassard, J.J. [Pompes Grundfos Distribution, 38 - Saint Quentin Fallavier (France)

    1998-03-01

    The reduction of the costs of variable speed pumps used in air-conditioning systems and the energy savings generated by such systems have made this technique attractive and adaptable to any size of installations. This paper presents the different regulation modes (constant differential pressure regulation, differential pressure regulation proportional to the flow rate, temperature regulation), the technology of cold water loops (primary and secondary circuits), and the regulation of the condenser loop (cooling tower, air flow and water flow regulation). (J.S.)

  3. Overall control strategy of variable speed doubly-fed induction generator wind turbine

    DEFF Research Database (Denmark)

    Hansen, A. D.; Soerensen, P.; Iov, Florin

    2004-01-01

    turbine. The present control method is designed for normal continuous operations. The strongest feature of the implemented control method is that it allows the turbine to operate with the optimum power efficiency over a wider range of wind speeds. The variable speed/variable pitch wind turbine with doubly......The variable speed doubly-fed induction generator wind turbine is today the most widely used concept. The paper presents an overall control system of the variable speed DFIG wind turbine, with focus on the control strategies and algorithms applied at each hierarchical control level of the wind...

  4. An examination of loads and responses of a wind turbine undergoing variable-speed operation

    Energy Technology Data Exchange (ETDEWEB)

    Wright, A.D.; Buhl, M.L. Jr.; Bir, G.S.

    1996-11-01

    The National Renewable Energy Laboratory has recently developed the ability to predict turbine loads and responses for machines undergoing variable-speed operation. The wind industry has debated the potential benefits of operating wind turbine sat variable speeds for some time. Turbine system dynamic responses (structural response, resonance, and component interactions) are an important consideration for variable-speed operation of wind turbines. The authors have implemented simple, variable-speed control algorithms for both the FAST and ADAMS dynamics codes. The control algorithm is a simple one, allowing the turbine to track the optimum power coefficient (C{sub p}). The objective of this paper is to show turbine loads and responses for a particular two-bladed, teetering-hub, downwind turbine undergoing variable-speed operation. The authors examined the response of the machine to various turbulent wind inflow conditions. In addition, they compare the structural responses under fixed-speed and variable-speed operation. For this paper, they restrict their comparisons to those wind-speed ranges for which limiting power by some additional control strategy (blade pitch or aileron control, for example) is not necessary. The objective here is to develop a basic understanding of the differences in loads and responses between the fixed-speed and variable-speed operation of this wind turbine configuration.

  5. Design and Construction of Variable Direct Current Speed Drive ...

    African Journals Online (AJOL)

    controlled rectifiers from the viewpoint of simplicity and cost effectiveness to act as power converter and controller. Design and construction of constituent circuits such as acceleration/deceleration, speed and current amplifier and the trigger ...

  6. Variable speed ac motor - A continuous pole printing technology

    Science.gov (United States)

    Morash, R. T.; Barber, R. J.; Roesel, J. F., Jr.

    A description is given of a simply constructed, brushless, commutatorless ac motor that offers significant improvements over conventional induction or synchronous motors. The design makes it possible for the speed to be precisely and readily varied and for the motor to operate with ac currents of any reasonable frequency over a wide range of voltages. In essence, it is a synchronous motor that can operate at speeds well above and below 'normal speed' synchronous conditions. When operated as a constant speed machine, its efficiency is such that it holds promise for reducing electrical energy consumption in the U.S. by 3 to 4 percent. Underlying the design is a magnetizable layer on the surface of the rotor that can be continuously remagnetized while the machine is in operation. A 25-hp motor developed for use with high inertia loads is discussed.

  7. Speed Sensorless Variable Structure Torque Control of Induction Motor

    OpenAIRE

    Jezernik, Karel; Šabanović, Asif; Rodič, Miran

    2010-01-01

    Induction motor speed sensorless torque control, which allows operation at low and zero speed, optimizing both torque response and efficiency, is proposed. The control is quite different than the conventional field-oriented or direct torque controls. A new discontinuous stator current FPGA based controller and rotor flux observer based on sliding mode and Lyapunov theory are developed, analyzed and experimentally verified. A smooth transition into the field weakening region and the full utili...

  8. Comprehensive Modeling and Analysis of Rotorcraft Variable Speed Propulsion System With Coupled Engine/Transmission/Rotor Dynamics

    Science.gov (United States)

    DeSmidt, Hans A.; Smith, Edward C.; Bill, Robert C.; Wang, Kon-Well

    2013-01-01

    This project develops comprehensive modeling and simulation tools for analysis of variable rotor speed helicopter propulsion system dynamics. The Comprehensive Variable-Speed Rotorcraft Propulsion Modeling (CVSRPM) tool developed in this research is used to investigate coupled rotor/engine/fuel control/gearbox/shaft/clutch/flight control system dynamic interactions for several variable rotor speed mission scenarios. In this investigation, a prototypical two-speed Dual-Clutch Transmission (DCT) is proposed and designed to achieve 50 percent rotor speed variation. The comprehensive modeling tool developed in this study is utilized to analyze the two-speed shift response of both a conventional single rotor helicopter and a tiltrotor drive system. In the tiltrotor system, both a Parallel Shift Control (PSC) strategy and a Sequential Shift Control (SSC) strategy for constant and variable forward speed mission profiles are analyzed. Under the PSC strategy, selecting clutch shift-rate results in a design tradeoff between transient engine surge margins and clutch frictional power dissipation. In the case of SSC, clutch power dissipation is drastically reduced in exchange for the necessity to disengage one engine at a time which requires a multi-DCT drive system topology. In addition to comprehensive simulations, several sections are dedicated to detailed analysis of driveline subsystem components under variable speed operation. In particular an aeroelastic simulation of a stiff in-plane rotor using nonlinear quasi-steady blade element theory was conducted to investigate variable speed rotor dynamics. It was found that 2/rev and 4/rev flap and lag vibrations were significant during resonance crossings with 4/rev lagwise loads being directly transferred into drive-system torque disturbances. To capture the clutch engagement dynamics, a nonlinear stick-slip clutch torque model is developed. Also, a transient gas-turbine engine model based on first principles mean

  9. Variable speed pumped storage system fed by large-scale cycloconverter

    Energy Technology Data Exchange (ETDEWEB)

    T, Taguchi. (The Tokyo Electric Power Co. Inc., Tokyo (Japan)); Mukai, K.; Yanagisawa, T.; Kanai, T. (Toshiba Corp., Tokyo (Japan))

    1992-01-01

    The world{prime}s first variable speed pumped storage system fed by large-scale cycloconverter was brought into operation at the Yagisawa power station Unit 2 of Tokyo Electric Power Co. in December, 1990. The present paper introduces an outline and operation results of this system. This remarkable system incorporates the latest large-scale, large-capacity power electronics technology, ultrahigh-speed digital control technology, and large-scale, large-capacity generator-motor technology. From the actual machine tests conducted at the Yagisawa power station, various functions of the variable speed pumped storage system were verified. The variable speed system enabled to regulate the input of the pump within the range of about 50 to 85MW. Additionally, in the turbine operation, vibration of the pump-turbine was improved by operating with the optimum rotating speed. Furthermore, the variable speed system did not cause unstable condition of power swing. 4 refs., 16 figs., 2 tabs.

  10. Simulation Analysis of SPWM Variable Frequency Speed Based on Simulink

    Directory of Open Access Journals (Sweden)

    Min-Yan DI

    2014-01-01

    Full Text Available This article is studied on currently a very active field of researching sinusoidal pulse width modulation (SPWM frequency speed control system, and strengthen researched on the simulation model of speed control system with MATLAB / Simulink / Power System simulation tools, thus we can find the best way to simulation. We apply it to the actual conveyor belt, frequency conversion motor, when the obtained simulation results are compared with the measured data, we prove that the method is practical and effective. The results of our research have a guiding role for the future engineering and technical personnel in asynchronous motor SPWM VVVF CAD design.

  11. Effect of running speed and leg prostheses on mediolateral foot placement and its variability.

    Directory of Open Access Journals (Sweden)

    Christopher J Arellano

    Full Text Available This study examined the effects of speed and leg prostheses on mediolateral (ML foot placement and its variability in sprinters with and without transtibial amputations. We hypothesized that ML foot placement variability would: 1. increase with running speed up to maximum speed and 2. be symmetrical between the legs of non-amputee sprinters but asymmetrically greater for the affected leg of sprinters with a unilateral transtibial amputation. We measured the midline of the body (kinematic data and center of pressure (kinetic data in the ML direction while 12 non-amputee sprinters and 7 Paralympic sprinters with transtibial amputations (6 unilateral, 1 bilateral ran across a range of speeds up to maximum speed on a high-speed force measuring treadmill. We quantified ML foot placement relative to the body's midline and its variability. We interpret our results with respect to a hypothesized relation between ML foot placement variability and lateral balance. We infer that greater ML foot placement variability indicates greater challenges with maintaining lateral balance. In non-amputee sprinters, ML foot placement variability for each leg increased substantially and symmetrically across speed. In sprinters with a unilateral amputation, ML foot placement variability for the affected and unaffected leg also increased substantially, but was asymmetric across speeds. In general, ML foot placement variability for sprinters with a unilateral amputation was within the range observed in non-amputee sprinters. For the sprinter with bilateral amputations, both affected legs exhibited the greatest increase in ML foot placement variability with speed. Overall, we find that maintaining lateral balance becomes increasingly challenging at faster speeds up to maximum speed but was equally challenging for sprinters with and without a unilateral transtibial amputation. Finally, when compared to all other sprinters in our subject pool, maintaining lateral balance

  12. Effect of running speed and leg prostheses on mediolateral foot placement and its variability.

    Science.gov (United States)

    Arellano, Christopher J; McDermott, William J; Kram, Rodger; Grabowski, Alena M

    2015-01-01

    This study examined the effects of speed and leg prostheses on mediolateral (ML) foot placement and its variability in sprinters with and without transtibial amputations. We hypothesized that ML foot placement variability would: 1. increase with running speed up to maximum speed and 2. be symmetrical between the legs of non-amputee sprinters but asymmetrically greater for the affected leg of sprinters with a unilateral transtibial amputation. We measured the midline of the body (kinematic data) and center of pressure (kinetic data) in the ML direction while 12 non-amputee sprinters and 7 Paralympic sprinters with transtibial amputations (6 unilateral, 1 bilateral) ran across a range of speeds up to maximum speed on a high-speed force measuring treadmill. We quantified ML foot placement relative to the body's midline and its variability. We interpret our results with respect to a hypothesized relation between ML foot placement variability and lateral balance. We infer that greater ML foot placement variability indicates greater challenges with maintaining lateral balance. In non-amputee sprinters, ML foot placement variability for each leg increased substantially and symmetrically across speed. In sprinters with a unilateral amputation, ML foot placement variability for the affected and unaffected leg also increased substantially, but was asymmetric across speeds. In general, ML foot placement variability for sprinters with a unilateral amputation was within the range observed in non-amputee sprinters. For the sprinter with bilateral amputations, both affected legs exhibited the greatest increase in ML foot placement variability with speed. Overall, we find that maintaining lateral balance becomes increasingly challenging at faster speeds up to maximum speed but was equally challenging for sprinters with and without a unilateral transtibial amputation. Finally, when compared to all other sprinters in our subject pool, maintaining lateral balance appears to be the

  13. Effect of Running Speed and Leg Prostheses on Mediolateral Foot Placement and Its Variability

    Science.gov (United States)

    Arellano, Christopher J.; McDermott, William J.; Kram, Rodger; Grabowski, Alena M.

    2015-01-01

    This study examined the effects of speed and leg prostheses on mediolateral (ML) foot placement and its variability in sprinters with and without transtibial amputations. We hypothesized that ML foot placement variability would: 1. increase with running speed up to maximum speed and 2. be symmetrical between the legs of non-amputee sprinters but asymmetrically greater for the affected leg of sprinters with a unilateral transtibial amputation. We measured the midline of the body (kinematic data) and center of pressure (kinetic data) in the ML direction while 12 non-amputee sprinters and 7 Paralympic sprinters with transtibial amputations (6 unilateral, 1 bilateral) ran across a range of speeds up to maximum speed on a high-speed force measuring treadmill. We quantified ML foot placement relative to the body’s midline and its variability. We interpret our results with respect to a hypothesized relation between ML foot placement variability and lateral balance. We infer that greater ML foot placement variability indicates greater challenges with maintaining lateral balance. In non-amputee sprinters, ML foot placement variability for each leg increased substantially and symmetrically across speed. In sprinters with a unilateral amputation, ML foot placement variability for the affected and unaffected leg also increased substantially, but was asymmetric across speeds. In general, ML foot placement variability for sprinters with a unilateral amputation was within the range observed in non-amputee sprinters. For the sprinter with bilateral amputations, both affected legs exhibited the greatest increase in ML foot placement variability with speed. Overall, we find that maintaining lateral balance becomes increasingly challenging at faster speeds up to maximum speed but was equally challenging for sprinters with and without a unilateral transtibial amputation. Finally, when compared to all other sprinters in our subject pool, maintaining lateral balance appears to be the

  14. Demonstration of variable speed permanent magnet generator at small, low-head hydro site

    Energy Technology Data Exchange (ETDEWEB)

    Brown Kinloch, David [Shaker Landing Hydro Associates, Inc., Louisville, KY (United States)

    2015-12-18

    Small hydro developers face a limited set of bad choices when choosing a generator for a small low-head hydro site. Direct drive synchronous generators are expensive and technically complex to install. Simpler induction generators are higher speed, requiring a speed increaser, which results in inefficiencies and maintenance problems. In addition, both induction and synchronous generators turn at a fixed speed, causing the turbine to run off its peak efficiency curve whenever the available head is different than the designed optimum head.The solution to these problems is the variable speed Permanent Magnet Generators (PMG). At the Weisenberger Mill in Midway, KY, a variable speed Permanent Magnet Generator has been installed and demonstrated. This new PMG system replaced an existing induction generator that had a HTD belt drive speed increaser system. Data was taken from the old generator before it was removed and compared to data collected after the PMG system was installed. The new variable speed PMG system is calculated to produce over 96% more energy than the old induction generator system during an average year. This significant increase was primarily due to the PMG generator operating at the correct speed at the maximum head, and the ability for the PMG generator to reduce its speed to lower optimum speeds as the stream flow increased and the net head decreased.This demonstration showed the importance of being able to adjust the speed of fixed blade turbines. All fixed blade turbines with varying net heads could achieve higher efficiencies if the speed can be matched to the optimum speed as the head changes. In addition, this demonstration showed that there are many potential efficiencies that could be realized with variable speed technology at hydro sites where mismatched turbine and generator speeds result in lower power output, even at maximum head. Funding for this project came from the US Dept. of Energy, through Award Number DE-EE0005429.

  15. Power Smoothing of a Variable-Speed Wind Turbine Generator in Association With the Rotor-Speed-Dependent Gain

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Yeonhee; Kang, Moses; Muljadi, Eduard; Park, Jung-Wook; Kang, Yong Cheol

    2017-07-01

    This paper proposes a power-smoothing scheme for a variable-speed wind turbine generator (WTG) that can smooth out the WTG's fluctuating power caused by varying wind speeds, and thereby keep the system frequency within a narrow range. The proposed scheme employs an additional loop based on the system frequency deviation that operates in conjunction with the maximum power point tracking (MPPT) control loop. Unlike the conventional, fixed-gain scheme, its control gain is modified with the rotor speed. In the proposed scheme, the control gain is determined by considering the ratio of the output of the additional loop to that of the MPPT loop. To improve the contribution of the scheme toward maintaining the frequency while ensuring the stable operation of WTGs, in the low rotor speed region, the ratio is set to be proportional to the rotor speed; in the high rotor speed region, the ratio remains constant. The performance of the proposed scheme is investigated under varying wind conditions for the IEEE 14-bus system. The simulation results demonstrate that the scheme successfully operates regardless of the output power fluctuation of a WTG by adjusting the gain with the rotor speed, and thereby improves the frequency-regulating capability of a WTG.

  16. Bearing Fault Diagnosis under Variable Speed Using Convolutional Neural Networks and the Stochastic Diagonal Levenberg-Marquardt Algorithm.

    Science.gov (United States)

    Tra, Viet; Kim, Jaeyoung; Khan, Sheraz Ali; Kim, Jong-Myon

    2017-12-06

    This paper presents a novel method for diagnosing incipient bearing defects under variable operating speeds using convolutional neural networks (CNNs) trained via the stochastic diagonal Levenberg-Marquardt (S-DLM) algorithm. The CNNs utilize the spectral energy maps (SEMs) of the acoustic emission (AE) signals as inputs and automatically learn the optimal features, which yield the best discriminative models for diagnosing incipient bearing defects under variable operating speeds. The SEMs are two-dimensional maps that show the distribution of energy across different bands of the AE spectrum. It is hypothesized that the variation of a bearing's speed would not alter the overall shape of the AE spectrum rather, it may only scale and translate it. Thus, at different speeds, the same defect would yield SEMs that are scaled and shifted versions of each other. This hypothesis is confirmed by the experimental results, where CNNs trained using the S-DLM algorithm yield significantly better diagnostic performance under variable operating speeds compared to existing methods. In this work, the performance of different training algorithms is also evaluated to select the best training algorithm for the CNNs. The proposed method is used to diagnose both single and compound defects at six different operating speeds.

  17. A Rotor Flux and Speed Observer for Sensorless Single-Phase Induction Motor Applications

    Directory of Open Access Journals (Sweden)

    Massimo Caruso

    2012-01-01

    Full Text Available It is usual to find single-phase induction motor (SPIM in several house, office, shopping, farm, and industry applications, which are become each time more sophisticated and requiring the development of efficient alternatives to improve the operational performance of this machine. Although the rotor flux and rotational speed are essential variables in order to optimize the operation of a SPIM, the use of conventional sensors to measure them is not a viable option. Thus, the adoption of sensorless strategies is the more reasonable proposal for these cases. This paper presents a rotor flux and rotational speed observer for sensorless applications involving SPIMs. Computer simulations and the experimental results are used to verify the performance of the proposed observer.

  18. Variable speed control for Vertical Axis Wind Turbine

    DEFF Research Database (Denmark)

    Galinos, Christos; Larsen, Torben J.

    and used after a first order low pass filtering with a certain time-constant. The controller has been developed and coded by Torben Larsen and it is compiled as an external DLL file. The simulations are done in the HAWC2 aero-servo-elastic code using a 3-bladed H-type VAWT which has been built within...... response on rotor speed and the produced power with a small overshoot in the power when the rated wind speed is reached for a wide range of PI gains for both the deterministic and the turbulent wind field. Lastly, it is not affected from the inherent variation in blade loading of VAWTs for each rotor...

  19. Bearing Stake Exercise: Sound Speed and other Environmental Variability

    Science.gov (United States)

    1978-09-01

    BEARING STAKE acoustics. DR. RALPH GOODMAN TECHNICAL DIRECTOR NORDA CONFIDENTIAL CONFIDENTIAL EXECUTIVE SUMMARY (C) This report contains an...acknowledgements are given to Dr. Robert R. Gardner of NOSC (BEARING STAKE Technical Director ) and to I)r. Aubrey L. Anderson of NORDA (Head of the...8217ISOUNDI I ý,INTERMEDIA TE SPEEM SOUN MINMUMCORRECTED MAXMU DEEP SOUND 20- CHA4NNELAXIS -2 NOTE - SOUND SPEED FROFILES FOR KPXBT 175,182 184 AND KP

  20. Variable-Speed Simulation of a Dual-Clutch Gearbox Tiltrotor Driveline

    Science.gov (United States)

    DeSmidt, Hans; Wang, Kon-Well; Smith, Edward C.; Lewicki, David G.

    2012-01-01

    This investigation explores the variable-speed operation and shift response of a prototypical two-speed dual-clutch transmission tiltrotor driveline in forward flight. Here, a Comprehensive Variable-Speed Rotorcraft Propulsion System Modeling (CVSRPM) tool developed under a NASA funded NRA program is utilized to simulate the drive system dynamics. In this study, a sequential shifting control strategy is analyzed under a steady forward cruise condition. This investigation attempts to build upon previous variable-speed rotorcraft propulsion studies by 1) including a fully nonlinear transient gas-turbine engine model, 2) including clutch stick-slip friction effects, 3) including shaft flexibility, 4) incorporating a basic flight dynamics model to account for interactions with the flight control system. Through exploring the interactions between the various subsystems, this analysis provides important insights into the continuing development of variable-speed rotorcraft propulsion systems.

  1. Energy Implications of Retrofitting Retail Sector Rooftop Units with Stepped-Speed and Variable-Speed Functionality

    Energy Technology Data Exchange (ETDEWEB)

    Studer, D.; Romero, R.; Herrmann, L.; Benne, K.

    2012-04-01

    Commercial retailers understand that retrofitting constant-speed RTU fan motors with stepped- or variable-speed alternatives could save significant energy in most U.S. climate zones. However, they lack supporting data, both real-world and simulation based, on the cost effectiveness and climate zone-specific energy savings associated with this measure. Thus, building managers and engineers have been unable to present a compelling business case for fan motor upgrades to upper management. This study uses whole-building energy simulation to estimate the energy impact of this type of measure so retailers can determine its economic feasibility.

  2. The History and State of the Art of Variable-Speed Wind Turbine Technology

    Energy Technology Data Exchange (ETDEWEB)

    Carlin, P.W.; Laxson, A.S.; Muljadi, E.B.

    2001-03-09

    The National Renewable Energy Laboratory's (NREL's) National Wind Technology Center (NWTC), directed by the Department of Energy (DOE) is pursuing several research projects in variable speed. In the near future the laboratory will be reevaluating its ongoing experiments and plans. The starting point for this reevaluation will be a sound understanding of the current state of the art in design and application of variable-speed technology. This report, which outlines current technologies and historical applications of variable-speed, will provide a baseline in deciding the course of research in the upcoming years.

  3. Periodic speed ripples minimization in PM synchronous motors using repetitive learning variable structure control.

    Science.gov (United States)

    Qian, Weizhe; Panda, S K; Xu, J X

    2003-10-01

    In this paper, we propose a simple repetitive learning variable structure control (RLVSC) scheme to reduce periodic speed ripples in a permanent magnet synchronous motor (PMSM). These speed ripples are induced by parasitic torque pulsations that vary periodically with rotor position. The conventional PI speed controller is able to reduce speed ripples to a certain extent but not sufficient enough for many high performance applications. During steady state, the RLVSC generates a reference compensation current that together with the outer loop PI speed controller is used to minimize the speed ripples. Being a plug-in module, the proposed RLVSC controller can be easily integrated to any of the existing PMSM drive systems. Experimental evaluation of the proposed scheme is carried out on a DSP-controlled PMSM drive platform. Test results obtained demonstrate the effectiveness of the proposed control scheme in reducing speed ripples by a factor of approximately 3 under various operating conditions.

  4. Advantages of adjustable speed drives; Les avantages des moteurs a vitesse variable

    Energy Technology Data Exchange (ETDEWEB)

    Menz, R. [Holcim Group Support Ltd., Holderbank AG (Switzerland); Opprecht, F. [Holcim Zement AG, Untervaz (Switzerland)

    2002-08-01

    The use of adjustable speed drives for process fan applications in our industry is already well established. However, a variety of solutions are possible due to different requirements and influences in a particular application. The paper describes the project concerning a conversion of a fan drive from fixed speed to variable speed. The different variants, which have been investigated, are stated and the final solution is shown in details. Furthermore, all relevant concerns for the final decision making such as costs, reliability and efficiency were analysed. The energy savings and process advantages by means of applying an adjustable speed drive are pointed out and a ROI calculation justifies the expenditures. (authors)

  5. Electromagnetic Design of a New Electrically Controlled Magnetic Variable-Speed Gearing Machine

    Directory of Open Access Journals (Sweden)

    Chunhua Liu

    2014-03-01

    Full Text Available This paper proposes a new electrically controlled magnetic variable-speed gearing (EC-MVSG machine, which is capable of providing controllable gear ratios for hybrid electric vehicle (HEV applications. The key design feature involves the adoption of a magnetic gearing structure and acceptance of the memory machine flux-mnemonic concept. Hence, the proposed machine can not only offer a gear-shifting mechanism for torque and speed transmission, but also provide variable gear ratios for torque and speed variation. The electromagnetic design is studied and discussed. The finite-element method is developed with the hysteresis model to verify the validity of the machine design.

  6. STATIC STRETCHING DOES NOT REDUCE VARIABILITY, JUMP AND SPEED PERFORMANCE.

    Science.gov (United States)

    de Oliveira, Fábio Carlos Lucas; Rama, Luís Manuel Pinto Lopes

    2016-04-01

    Stretching is often part of the warm-up routine prior to athletic participation; however, controversial evidence exists on the effects of stretching on countermovement jump (CMJ) and sprint performance. Additionally, analysis of variability between repeated tasks is useful for monitoring players, to analyze factors that could affect the performance, and to guide clinical decisions for training strategies. The purpose of this study was to examine whether static stretching (SS) prior to CMJ and 20-meter (20-m) sprint would affect performance, and to investigate whether SS affects an athlete's ability to perform these tasks consistently. Twenty-two trained healthy athletes (23.2 ± 5.0 years) attended, randomly, two testing sessions, separated by 48 hours. At session one, all participants underwent 10 minutes of dynamic running warm-up followed by the experimental tasks (three CMJ and three 20-m sprint), whereas five minutes of stretching was added after the warm-up routine at session two. All participants performed the same experimental tasks in both sessions. The stretching protocol consisted of five stretching exercises for each lower limb. The paired-samples t-test revealed no significant differences between the stretching protocol condition and no stretching condition for the 20-m sprint (t(21)=.920; p=.368) and CMJ (t(21)=.709; p=.486). There were no significant differences in trial-by-trial variability on 20-m sprint (t(21)=1.934; p=.067) and CMJ scores (t(21)=.793; p=.437) as result of SS. The SS protocol did not modify jumping and running ability in trained healthy athletes. The SS prior to training or competition may not cause detrimental effects to athletic performance. Level III, Nonrandomized controlled trial.

  7. Modelling and control of variable speed wind turbines for power system studies

    DEFF Research Database (Denmark)

    Michalke, Gabriele; Hansen, Anca Daniela

    2010-01-01

    and implemented in the power system simulation tool DIgSILENT. Important issues like the fault ride-through and grid support capabilities of these wind turbine concepts are addressed. The paper reveals that advanced control of variable speed wind turbines can improve power system stability. Finally......Modern wind turbines are predominantly variable speed wind turbines with power electronic interface. Emphasis in this paper is therefore on the modelling and control issues of these wind turbine concepts and especially on their impact on the power system. The models and control are developed......, it will be shown in the paper that wind parks consisting of variable speed wind turbines can help nearby connected fixed speed wind turbines to ride-through grid faults. Copyright © 2009 John Wiley & Sons, Ltd....

  8. Very-low-speed variable-structure control of sensorless induction machine drives without signal injection

    DEFF Research Database (Denmark)

    Lascu, Christian; Boldea, Ion; Blaabjerg, Frede

    2005-01-01

    A sensorless induction machine drive is presented, in which the principles of variable-structure control and direct torque control (DTC) are combined to ensure high-performance operation in the steady state and under transient conditions. The drive employs a new torque and flux controller......, the "linear and variable-structure control", which realizes accurate and robust control in a wide speed range. Conventional DTC transient merits are preserved, while the steady-state behavior is significantly improved. The full-order state observer is a sliding-mode one, which does not require the rotor speed...... adaptation and provides accurate state estimation in the entire speed range. The proposed scheme is a complete variable-structure solution that allows persistent sensorless operation of the drive at very low speeds, including zero and 3 r/min, with full load. Simulations and extensive experimental results...

  9. A Variable Single Photon Plasmonic Beamsplitter

    DEFF Research Database (Denmark)

    Israelsen, Niels Møller; Kumar, Shailesh; Huck, Alexander

    Plasmonic structures can both be exploited for scaling down optical components beyond the diffraction limit and enhancing andcollecting the emission from a single dipole emitter. Here, we experimentally demonstrate adiabatic coupling between two silvernanowires using a nitrogen vacancy center as ...

  10. High Performance Variable Speed Drive System and Generating System with Doubly Fed Machines

    Science.gov (United States)

    Tang, Yifan

    Doubly fed machines are another alternative for variable speed drive systems. The doubly fed machines, including doubly fed induction machine, self-cascaded induction machine and doubly excited brushless reluctance machine, have several attractive advantages for variable speed drive applications, the most important one being the significant cost reduction with a reduced power converter rating. With a better understanding, improved machine design, flexible power converters and innovated controllers, the doubly fed machines could favorably compete for many applications, which may also include variable speed power generations. The goal of this research is to enhance the attractiveness of the doubly fed machines for both variable speed drive and variable speed generator applications. Recognizing that wind power is one of the favorable clean, renewable energy sources that can contribute to the solution to the energy and environment dilemma, a novel variable-speed constant-frequency wind power generating system is proposed. By variable speed operation, energy capturing capability of the wind turbine is improved. The improvement can be further enhanced by effectively utilizing the doubly excited brushless reluctance machine in slip power recovery configuration. For the doubly fed machines, a stator flux two -axis dynamic model is established, based on which a flexible active and reactive power control strategy can be developed. High performance operation of the drive and generating systems is obtained through advanced control methods, including stator field orientation control, fuzzy logic control and adaptive fuzzy control. System studies are pursued through unified modeling, computer simulation, stability analysis and power flow analysis of the complete drive system or generating system with the machine, the converter and the control. Laboratory implementations and tested results with a digital signal processor system are also presented.

  11. Measurement of reading speed with standardized texts: a comparison of single sentences and paragraphs.

    Science.gov (United States)

    Altpeter, Elke Karin; Marx, Tobias; Nguyen, Nhung Xuan; Naumann, Aline; Trauzettel-Klosinski, Susanne

    2015-08-01

    We examined the influence of text length (single sentence versus a paragraph of several sentences) on the repeatability of reading speed measurements in normal-sighted subjects. We compared reading speeds for the German versions of the Radner charts (single sentences of 14 words each) and the International Reading Speed Texts (IReST) charts (paragraphs, on average 132 words) in 30 normal-sighted elderly subjects aged 51-81 years (mean 64.5 years ± 7.2 SD). Three texts each of both lengths were read aloud in random order. The influence of text length (single sentence or paragraph) and text sample (each single text) on reading speed was calculated by a regression model and Bland-Altman analysis. Mean reading speed (words per minute) showed no significant difference for single sentences (170 wpm ± 33 SD) and paragraphs (167 wpm ±31 SD). Differences in reading speeds within one type of reading material were higher between single sentences than between paragraphs. Correlation coefficients between speeds were higher for paragraphs (r = 0.96-0.98) than for single sentences (r = 0.69-0.78). Variations between reading speeds for three texts of each length were markedly lower for paragraphs than for single sentences: (median, interquartile range [IQR]): 6.7, IQR 13.9; 3.0, IQR 8.3; -2.0, IQR 9.7 versus -8.8, IQR 29.6; 15.6, IQR 29.4; 22.7, IQR 19.4, respectively. Since reading speeds assessed with paragraphs show lower variance among texts than those for single sentences, they are better suited for repeated measurements, especially for long-term monitoring of the course of reading performance and for assessing effects of interventions in subjects with reading disorders.

  12. Quantifying and optimizing single-molecule switching nanoscopy at high speeds.

    Directory of Open Access Journals (Sweden)

    Yu Lin

    Full Text Available Single-molecule switching nanoscopy overcomes the diffraction limit of light by stochastically switching single fluorescent molecules on and off, and then localizing their positions individually. Recent advances in this technique have greatly accelerated the data acquisition speed and improved the temporal resolution of super-resolution imaging. However, it has not been quantified whether this speed increase comes at the cost of compromised image quality. The spatial and temporal resolution depends on many factors, among which laser intensity and camera speed are the two most critical parameters. Here we quantitatively compare the image quality achieved when imaging Alexa Fluor 647-immunolabeled microtubules over an extended range of laser intensities and camera speeds using three criteria - localization precision, density of localized molecules, and resolution of reconstructed images based on Fourier Ring Correlation. We found that, with optimized parameters, single-molecule switching nanoscopy at high speeds can achieve the same image quality as imaging at conventional speeds in a 5-25 times shorter time period. Furthermore, we measured the photoswitching kinetics of Alexa Fluor 647 from single-molecule experiments, and, based on this kinetic data, we developed algorithms to simulate single-molecule switching nanoscopy images. We used this software tool to demonstrate how laser intensity and camera speed affect the density of active fluorophores and influence the achievable resolution. Our study provides guidelines for choosing appropriate laser intensities for imaging Alexa Fluor 647 at different speeds and a quantification protocol for future evaluations of other probes and imaging parameters.

  13. Method for estimating energy saving by variable-speed control of centrifugal pump

    Energy Technology Data Exchange (ETDEWEB)

    Chantasiriwan, S. [Thammasat Univ., Pathum Thani (Thailand). Faculty of Engineering

    2009-03-11

    Flow rates are often varied in centrifugal pump applications. This paper presented a simplified model of a centrifugal pump that used a minimal number of pump and system parameters. The model was designed to calculate energy savings caused by using variable speed controls and to determine accurate payback periods. The model assumed that the pump head was a quadratic function of flow rate, and that pump efficiency was a cubic function of flow rate. Dimensionless pump characteristics were characterized as functions of dimensionless flow rate at an arbitrary pump speed in order to prepare a pump power construction chart. The chart was then used to compute the power consumption of variable speed pumps. Results of the study demonstrated that as the static head of the pumping system increases, the deviation rate from the affinity laws increased. The study demonstrated that the model can be used to compute the power required to operate a pump at arbitrary speeds. 9 refs., 6 figs.

  14. A control strategy for a variable-speed wind energy conversion system

    Science.gov (United States)

    Jacob, A.; Rajagopalan, V.; Veillette, D.

    1980-01-01

    In this article, a method of calculating an optimal control strategy for a variable-speed wind power generation scheme incorporating a squirrel cage induction machine and operating in a self-excited induction generator mode is discussed. This scheme also uses a conventional three-phase thyristor rectifier, a line-commutated inverter and an economical auxiliary commutated-voltage-source inverter. The three regulated variables are: (1) drive speed as a function of available mechanical energy by manipulating the resistive torque developed by induction generator; (2) induction motor power consumption during start-up of the wind machine of vertical axis type; (3) operating slip of the induction machine, thereby limiting start-up and braking currents. The developed strategy is also suitable for any other variable-speed drive system incorporating an induction machine.

  15. Supervisory control of a variable speed wind turbine with doubly fed induction generator

    Directory of Open Access Journals (Sweden)

    C. Viveiros

    2015-11-01

    Full Text Available This paper is on an onshore variable speed wind turbine with doubly fed induction generator and under supervisory control. The control architecture is equipped with an event-based supervisor for the supervision level and fuzzy proportional integral or discrete adaptive linear quadratic as proposed controllers for the execution level. The supervisory control assesses the operational state of the variable speed wind turbine and sends the state to the execution level. Controllers operation are in the full load region to extract energy at full power from the wind while ensuring safety conditions required to inject the energy into the electric grid. A comparison between the simulations of the proposed controllers with the inclusion of the supervisory control on the variable speed wind turbine benchmark model is presented to assess advantages of these controls.

  16. Variable speed wind turbine generator system with current controlled voltage source inverter

    Energy Technology Data Exchange (ETDEWEB)

    Muyeen, S.M., E-mail: muyeen0809@yahoo.co [Dept. of Electrical Engineering, Petroleum Institute, P.O. Box 2533, Abu Dhabi (United Arab Emirates); Al-Durra, Ahmed [Dept. of Electrical Engineering, The Petroleum Institute, P.O. Box 2533, Abu Dhabi (United Arab Emirates); Tamura, J. [Dept. of EEE, Kitami Institute of Technology, 165 Koen-cho, Kitami 090-8507 (Japan)

    2011-07-15

    highlights: {yields} Current controlled voltage source inverter scheme for wind power application. {yields} Low voltage ride through of wind farm. {yields} Variable speed wind turbine driven permanent magnet synchronous generator-operation and control. -- Abstract: The present popular trend of wind power generation is to use variable speed wind turbine (VSWT) driving a doubly fed induction generator (DFIG), wound field synchronous generator (WFSG) or permanent magnet synchronous generator (PMSG). Among them, stability analyses of DFIG type of VSWT have already been reported in many literatures. However, transient stability and low voltage ride through (LVRT) characteristics analyses for synchronous generator type of VSWT is not sufficient enough. This paper focuses on detailed LVRT characteristic analysis of variable speed wind turbine driving a PMSG (VSWT-PMSG) with current controlled voltage source inverter (CC-VSI). Modeling and suitable control strategies for overall system are developed to augment the low voltage ride through capability of variable speed wind generator, considering recent wind farm grid code. Both symmetrical and unsymmetrical faults are analyzed as network disturbances in this paper. The permanent fault due to unsuccessful reclosing of circuit breakers is taken into consideration, which is a salient feature of this study. Moreover, the dynamic characteristic is analyzed using real wind speed data measured in Hokkaido Island, Japan. The proposed control scheme is simulated by using the standard power system simulation package PSCAD/EMTDC and results are verified by comparing that of voltage controlled voltage source inverter scheme available in power system literature.

  17. Comparative analysis of single- and continuously variable-capacity heat pump concepts

    Energy Technology Data Exchange (ETDEWEB)

    Rice, C.K.; Fischer, S.K.

    1984-01-01

    This work is an initial assessment of the potential benefits of continuous-capacity-modulation in electric-driven, air-to-air heat pumps for residential application. The purpose of the project was to provide a quantitative estimate of the possible annual performance gains of advanced continuously modulating heat pumps relative to single-speed designs at comparable levels of development. Previous analytical design work in this area at ORNL dealt with single-design-point, heating-mode optimization of single-speed heat pumps. For that work the ORNL Steady State Heat Pump Design Model was connected to a constrained numerical optimization code. The present work represents an extension of the earlier work in two directions. First, seasonal (heating and cooling) and annual performance factor (APF) analysis capability was added to allow direct evaluation of annual energy use from heat pump performance data generated by the ORNL heat pump model. Secondly, a modulating version of the heat pump model was developed to provide a means for simulating the steady state performance of continuously variable-speed (CVS) systems. With these tools, the APFs of both single- and continuously variable-capacity (CVC) concepts could be studied as basic heat pump design variables were varied. Based on this initial evaluation of CVS systems and considering the potential for electronics costs to further decrease as electricity prices rise, we see such advanced CVS systems as a strong future competitor to single-speed systems and as decidedly superior in energy conservation potential. To achieve this position, it seems especially important that such systems be computer optimized to take full advantage of the increased design flexibility available. Further, development of PM-ECMs or equivalent technology must continue to the point of providing speed controllers of: moderate to low cost, moderate to high performance, and high reliability. 16 refs., 12 figs.

  18. Automated Bearing Fault Diagnosis Using 2D Analysis of Vibration Acceleration Signals under Variable Speed Conditions

    Directory of Open Access Journals (Sweden)

    Sheraz Ali Khan

    2016-01-01

    Full Text Available Traditional fault diagnosis methods of bearings detect characteristic defect frequencies in the envelope power spectrum of the vibration signal. These defect frequencies depend upon the inherently nonstationary shaft speed. Time-frequency and subband signal analysis of vibration signals has been used to deal with random variations in speed, whereas design variations require retraining a new instance of the classifier for each operating speed. This paper presents an automated approach for fault diagnosis in bearings based upon the 2D analysis of vibration acceleration signals under variable speed conditions. Images created from the vibration signals exhibit unique textures for each fault, which show minimal variation with shaft speed. Microtexture analysis of these images is used to generate distinctive fault signatures for each fault type, which can be used to detect those faults at different speeds. A k-nearest neighbor classifier trained using fault signatures generated for one operating speed is used to detect faults at all the other operating speeds. The proposed approach is tested on the bearing fault dataset of Case Western Reserve University, and the results are compared with those of a spectrum imaging-based approach.

  19. Flicker Study on Variable Speed Wind Turbines with Permanent Magnet Synchronous Generator

    DEFF Research Database (Denmark)

    Hu, Weihao; Chen, Zhe; Wang, Yue

    2008-01-01

    Grid connected wind turbines are fluctuating power sources that may produce flicker during continuous operation. This paper presents a simulation model of a MW-level variable speed wind turbines with a permanent magnet synchronous generator (PMSG) and a full-scale converter developed in the simul......Grid connected wind turbines are fluctuating power sources that may produce flicker during continuous operation. This paper presents a simulation model of a MW-level variable speed wind turbines with a permanent magnet synchronous generator (PMSG) and a full-scale converter developed...

  20. Intelligent Multiobjective Slip and Speed Ratio Control of a Novel Dual-Belt Continuously Variable Transmission for Automobiles

    Directory of Open Access Journals (Sweden)

    Zhengchao Xie

    2014-01-01

    Full Text Available Van Doorne’s continuously variable transmission (CVT is the most popular CVT design for automotive transmission, but it is only applicable to low-power passenger cars because of its low torque capacity. To overcome this limitation of traditional single-belt CVT, a novel dual-belt Van Doorne’s CVT (DBVCVT system, which is applicable to heavy-duty vehicles, has been previously proposed by the authors. This paper, based on the published analytical model and test rig of DBVCVT, further proposes an intelligent multiobjective fuzzy controller for slip and speed ratio control of DBVCVT. The controller aims to safely control the clamping forces of both the primary and the secondary pulleys in order to improve the transmission efficiency, achieve the accurate speed ratio, and avoid the belt slip under different engine loads and vehicle speeds. The slip, speed ratio, and transmission efficiency dynamics of DBVCVT are firstly analyzed and modeled in this paper. With the aid of a flexible objective function, the analytical model, and fuzzy logic, a Pareto rule base for fuzzy controller is developed for multiobjective DBVCVT control. Experimental results show that the proposed controller for slip and speed ratio regulation of DBVCVT is effective and performs well under different user-defined weights.

  1. Intraindividual variability in neurocognitive speed: a comparison of Parkinson's disease and normal older adults.

    Science.gov (United States)

    de Frias, Cindy M; Dixon, Roger A; Fisher, Nancy; Camicioli, Richard

    2007-06-18

    We examined whether intraindividual variability of neurocognitive speed, or inconsistency, is greater in stages of Parkinson's disease (PD) as compared to a matched group of normal older adults. Intraindividual variability was assessed using four reaction time (RT) (simple and complex) tasks. We examined three sets of correlates: executive functioning (Stroop (interference index), Trail Making Test (Part B), and Digit Ordering Test), finger tapping speed, and gait speed. The participants were matched on age, sex, and education, and did not differ in global cognitive functioning. There were 50 patients with a clinical diagnosis of idiopathic PD (29 men and 21 women) who ranged from 65 to 84 years (M=71.5, S.D.=4.7) and 48 matched healthy older adults who ranged from 65 to 84 years (M=71.5, S.D.=4.9). Multiple analyses of variance showed that the PD patients were slower on all three complex RT tasks, and more inconsistent than healthy older adults on the most complex (eight-choice) RT task. Individuals with advanced disease had slower neurocognitive speed and more inconsistency than patients with earlier stage PD. Poorer executive functioning was associated with slower neurocognitive performance in healthy older adults, mild PD patients, and especially severe PD patients. Greater inconsistency in speed was related to poorer executive functioning in late stage PD (for the most complex task) and in healthy older adults (for the simplest task), indicating that motor and cognitive domains have functional coupling (i.e., as one becomes compromised so does the other). Intraindividual variability was not correlated with tapping speed and gait speed in any group. Executive functioning and neurocognitive speed may be valid and distinct clinical markers of disease progression in PD.

  2. Influence of Global Climate Indices and Inter-annual Wind Speed Variability in the UAE

    Science.gov (United States)

    Naizghi, M. S.; Ouarda, T.

    2016-12-01

    Wind is becoming one of the world's most favourable source of sustainable energy. Its stochastic nature, however, is a challenge to fully exploit its potential. To overcome this limitation, a number of studies focusing on modelling the character of wind were conducted but few explored the effect of large-scale climatological factors on wind distribution and variation. The present study aims to: first, examine the variability and change points in wind speed time series data. Second, identify the teleconnections between the wind speed and climate indices by applying wavelet coherence analysis. Third, develop multiple linear regression models to represent wind speed as a function of climate indices. The study is carried out using wind speed data collected from six ground stations in the UAE and a reanalysis gridded dataset. Continuous wavelet analysis on the wind speed indicated annual periodicity in all stations and biannual in three. Trend analysis using Modified Mann-Kendall test and linear regression showed that half of the stations have a significant trend at the 5% level. Cumulative sum and Bayesian change detection analysis revealed all stations, except one, exhibited change points with statistical significance level of 5%. Wavelet coherence analysis demonstrated that wind speed in the UAE is mainly associated with the North Atlantic Oscillation (NAO), East Atlantic Oscillation (EAO), El Niño Southern Oscillation (ENSO) and the Indian Ocean Dipole (IOD). NAO and EAO simultaneously modulate wind speed in the summer while ENSO and IOD influence winter and autumn wind speeds. Step-wise multiple linear regression models developed to select appropriate predictors among the various climate indices resulted in limited success. Further work on predictive models using non-linear models and generalized additive model are being explored. Keywords: Climate indices, teleconnections, wavelet analysis, wind speed variability, trend analysis, change point, UAE.

  3. The high-speed operation of single phase switched reluctance motor considering magnetic saturation

    Science.gov (United States)

    Ahn, Joonseon; Won, Sung Hong; Lee, Ju

    2006-04-01

    In the high-speed operation of SRM, the conventional pulse width modulation (PWM) drive method is not available because of the limitation of switching speed; therefore the single-pulse drive method is commonly employed. On the contrary, the use of the single-pulse drive method cannot avoid the overcurrent in the low-speed operation because of the insufficient back emf and the difficulty of duty control. With these reasons, the switching method is commonly changed from PWM at the low-speed operation to the single-pulse method at the high-speed operation. In the fan application, the required load torque increases as a square of the fan speed; it requires more current for the torque generation. Therefore at the mode transition between PWM and single-pulse drive, it is unavoidable that the phase current rapidly increases if the nonlinearity of inductance to the current is not considered. In this paper, by using finite element method (FEM) which is considered with the nonlinearity of the inductance with respect to the current, the speed of mode transition is calculated (18 000 rpm) and verified by the experiment.

  4. Robust Active Disturbance Rejection Control Approach to Maximize Energy Capture in Variable-Speed Wind Turbines

    Directory of Open Access Journals (Sweden)

    Horacio Coral-Enriquez

    2013-01-01

    Full Text Available This paper proposes an alternative robust observer-based linear control technique to maximize energy capture in a 4.8 MW horizontal-axis variable-speed wind turbine. The proposed strategy uses a generalized proportional integral (GPI observer to reconstruct the aerodynamic torque in order to obtain a generator speed optimal trajectory. Then, a robust GPI observer-based controller supported by an active disturbance rejection (ADR approach allows asymptotic tracking of the generator speed optimal trajectory. The proposed methodology controls the power coefficient, via the generator angular speed, towards an optimum point at which power coefficient is maximum. Several simulations (including an actuator fault are performed on a 4.8 MW wind turbine benchmark model in order to validate the proposed control strategy and to compare it to a classical controller. Simulation and validation results show that the proposed control strategy is effective in terms of power capture and robustness.

  5. Spatial and temporal variability of mean daily wind speeds in the Czech Republic, 1961-2015

    Czech Academy of Sciences Publication Activity Database

    Brázdil, Rudolf; Zahradníček, Pavel; Řezníčková, Ladislava; Tolasz, R.; Štěpánek, Petr; Dobrovolný, Petr

    2017-01-01

    Roč. 72 (2017), s. 197-216 ISSN 0936-577X R&D Projects: GA MŠk(CZ) LO1415; GA ČR(CZ) GA15-11805S Institutional support: RVO:67179843 Keywords : mean daily wind speed * spatial variability * temporal variability * wind stilling * Czech Republic Subject RIV: DG - Athmosphere Sciences, Meteorology Impact factor: 1.578, year: 2016

  6. Low Voltage Ride-Through of Variable Speed Wind Turbines with Permanent Magnet Synchronous Generator

    DEFF Research Database (Denmark)

    Hu, Weihao; Chen, Zhe; Wang, Yue

    2009-01-01

    This paper presents a simulation model of a MW-level variable speed wind turbine with a permanent magnet synchronous generator (PMSG) and a full-scale converter developed in the simulation tool of PSCAD/EMTDC. The low voltage ride-through (LVRT) capability of the wind turbine is investigated. A new...

  7. Dynamic Analysis of Fluid Power Drive-trains for Variable Speed Wind Turbines : A Parameter Study

    NARCIS (Netherlands)

    Jarquin Laguna, A.; Diepeveen, N.F.B.

    2013-01-01

    In the pursuit of making wind energy technology more economically attractive, the application of fluid power technology for the transmission of wind energy is being developed by several parties all over the world. This paper presents a dynamic model of a fluid power transmission for variable speed

  8. FUZZY LOGIC BASED INTELLIGENT CONTROL OF A VARIABLE SPEED CAGE MACHINE WIND GENERATION SYSTEM

    Science.gov (United States)

    The report gives results of a demonstration of the successful application of fuzzy logic to enhance the performance and control of a variable-speed wind generation system. A squirrel cage induction generator feeds the power to either a double-sided pulse-width modulation converte...

  9. Wind tunnel experiments to prove a hydraulic passive torque control concept for variable speed wind turbines

    NARCIS (Netherlands)

    Diepeveen, N.F.B.; Jarquin-Laguna, A.

    2014-01-01

    In this paper the results are presented of experiments to prove an innovative concept for passive torque control of variable speed wind turbines using fluid power technology. It is demonstrated that by correctly configuring the hydraulic drive train, the wind turbine rotor operates at or near

  10. Models for Numerical Evaluation of Variable Speed Different Wind Generator Systems

    DEFF Research Database (Denmark)

    Li, Hui; Chen, Zhe; Polinder, H.

    2007-01-01

    of different wind generator systems, the other presents the optimization results and evaluation of variable speed wind generator systems. In this report, firstly, it gives an overview of various wind generator topologies, including their advantages and disadvantages, market status and developing trends. Next...

  11. Variability in the Speed of the Brewer-Dobson Circulation as Observed by Aura/MLS

    Science.gov (United States)

    Flury, Thomas; Wu, Dong L.; Read, W. G.

    2013-01-01

    We use Aura/MLS stratospheric water vapour (H2O) measurements as tracer for dynamics and infer interannual variations in the speed of the Brewer-Dobson circulation (BDC) from 2004 to 2011. We correlate one-year time series of H2O in the lower stratosphere at two subsequent pressure levels (68 hPa, approx.18.8 km and 56 hPa, approx 19.9 km at the Equator) and determine the time lag for best correlation. The same calculation is made on the horizontal on the 100 hPa (approx 16.6 km) level by correlating the H2O time series at the Equator with the ones at 40 N and 40 S. From these lag coefficients we derive the vertical and horizontal speeds of the BDC in the tropics and extra-tropics, respectively. We observe a clear interannual variability of the vertical and horizontal branch. The variability reflects signatures of the Quasi Biennial Oscillation (QBO). Our measurements confirm the QBO meridional circulation anomalies and show that the speed variations in the two branches of the BDC are out of phase and fairly well anti-correlated. Maximum ascent rates are found during the QBO easterly phase. We also find that transport of H2O towards the Northern Hemisphere (NH) is on the average two times faster than to the Southern Hemisphere (SH) with a mean speed of 1.15m/s at 100 hPa. Furthermore, the speed towards the NH shows much more interannual variability with an amplitude of about 21% whilst the speed towards the SH varies by only 10 %. An amplitude of 21% is also observed in the variability of the ascent rate at the Equator which is on the average 0.2mm/s.

  12. Development of Permanent Magnet Reluctance Motor Suitable for Variable-Speed Drive for Electric Vehicle

    Science.gov (United States)

    Sakai, Kazuto; Takahashi, Norio; Shimomura, Eiji; Arata, Masanobu; Nakazawa, Yousuke; Tajima, Toshinobu

    Regarding environmental and energy issues, increasing importance has been placed on energy saving in various systems. To save energy, it would be desirable if the total efficiency of various types of equipment were increased.Recently, a hybrid electric vehicle (HEV) and an electric vehicle (EV) have been developed. The use of new technologies will eventually lead to the realization of the new- generation vehicle with high efficiency. One new technology is the variable-speed drive over a wide range of speeds. The motor driving systems of the EV or the HEV must operate in the variable-speed range of up to 1:5. This has created the need for a high-efficiency motor that is capable of operation over a wide speed range. In this paper, we describe the concept of a novel permanent magnet reluctance motor (PRM) and discuss its characteristics. We developed the PRM, which has the capability of operating over a wide speed range with high efficiency. The PRM has a rotor with a salient pole, which generates magnetic anisotropy. In addition, the permanent magnets embedded in the rotor core counter the q-axis flux by the armature reaction. Then, the power density and the power factor increase. The PRM produces reluctance torque and torque by permanent magnet (PM) flux. The reluctance torque is 1 to 2 times larger than the PM torque. When the PRM operates over a constant-power speed range, the field component of the current will be regulated to maintain a constant voltage. The output power of the developed PRM is 8 to 250kW. It is clarified that the PRM operates at a wide variable-speed range (1:5) with high efficiency (92-97%). It is concluded that the PRM has high performance over a wide constant-power speed range. In addition, the PRM is constructed using a small PM, so that we can solve the problem of cost. Thus, the PRM is a superior machine that is suited for variable-speed drive applications.

  13. Overuse Injuries Associated with Mountain Biking: Is Single-Speed Riding a Predisposing Factor?

    Directory of Open Access Journals (Sweden)

    Michael T. Lebec

    2014-01-01

    Full Text Available Though mountain bikers are at significant risk for overuse injury, there is minimal quality research describing this relationship. Single-speed mountain biking, in which participants pedal a bike with only a single gear, may place riders at even greater risk for overuse problems due to the disproportionate physical effort associated with this type of riding. The focus of this study was to provide additional perspective on overuse injuries sustained by mountain bikers and to determine if single-speed mountain biking places participants at greater risk for overuse conditions. Four hundred and four (404 mountain bikers were surveyed concerning overuse injuries sustained during the previous year. Findings indicate that 63% of respondents reported an overuse injury affecting at least one area with the most commonly reported areas being the lumbar spine, knees, hand/wrist, and cervical spine. Individuals riding single-speed mountain bikes did not have a higher incidence of overuse injuries than riders of multiple-geared bikes. However, respondents who split time between riding single-speed and multiple-geared bikes were significantly more likely to report an overuse syndrome than those only riding single-speed or multiple-geared bikes (p = 0.0104. This group of riders may be at greater risk for overuse injury due to excessive fatigue and poor biomechanics.

  14. High transonic speed transport aircraft study. [aerodynamic characteristics of single-fuselage, yawed-wing configuration

    Science.gov (United States)

    Kulfan, R. M.; Neumann, F. D.; Nisbet, J. W.; Mulally, A. R.; Murakami, J. K.; Noble, E. C.; Mcbarron, J. P.; Stalter, J. L.; Gimmestad, D. W.; Sussman, M. B.

    1973-01-01

    An initial design study of high-transonic-speed transport aircraft has been completed. Five different design concepts were developed. These included fixed swept wing, variable-sweep wing, delta wing, double-fuselage yawed-wing, and single-fuselage yawed-wing aircraft. The boomless supersonic design objectives of range=5560 Km (3000 nmi), payload-18 143 kg (40 000lb), Mach=1.2, and FAR Part 36 aircraft noise levels were achieved by the single-fuselage yawed-wing configuration with a gross weight of 211 828 Kg (467 000 lb). A noise level of 15 EPNdB below FAR Part 36 requirements was obtained with a gross weight increase to 226 796 Kg (500 000 lb). Although wing aeroelastic divergence was a primary design consideration for the yawed-wing concepts, the graphite-epoxy wings of this study were designed by critical gust and maneuver loads rather than by divergence requirements. The transonic nacelle drag is shown to be very sensitive to the nacelle installation. A six-degree-of-freedom dynamic stability analysis indicated that the control coordination and stability augmentation system would require more development than for a symmetrical airplane but is entirely feasible. A three-phase development plan is recommended to establish the full potential of the yawed-wing concept.

  15. Image Reconstruction in Magnetoacoustic Tomography With Magnetic Induction With Variable Sound Speeds.

    Science.gov (United States)

    Zhang, Wei; Ma, Ren; Zhang, Shunqi; Yin, Tao; Liu, Zhipeng

    2016-12-01

    Acoustic and electrical characteristics of biological tissue are important factors in magnetoacoustic tomography with magnetic induction (MAT-MI). Acoustic inhomogeneity significantly affects the propagations of sound waves. Differences in sound speed lead to distortions of the sound sources in the reconstruction process. The objective of this study is to develop a novel algorithm to reconstruct the sound source distribution in an acoustically inhomogeneous medium. The proposed algorithm is developed on the basis of the finite-difference time-domain method and time-reversal acoustic theory; it combines the relationship among symmetrical transducers with the back-projection algorithm. An acoustically inhomogeneous model with different regions of variable sound speeds is established to validate the proposed algorithm. From the data collected by a rotated focused transducer, first, the sound speed distribution is reconstructed, and then, the sound sources of the model are reconstructed. The reconstructed sound sources are obviously distorted when the speed differences are not considered. In contrast, the proposed algorithm yields reconstructed sound sources that are consistent with the model in terms of shape and size. Thus, the proposed algorithm is capable of accurately reconstructing the acoustic sources distribution in an acoustically inhomogeneous medium. This method provides a solution reducing the influence of acoustic inhomogeneity in MAT-MI. The distributions of sound speed can be obtained during the process of reconstructing the sound source. Consequently, the imaging of the acoustic speed and the electrical conductivity of biological tissues can be implemented simultaneously in MAT-MI.

  16. Energy and economic analysis of an ICE-based variable speed-operated micro-cogenerator

    Energy Technology Data Exchange (ETDEWEB)

    Caresana, Flavio; Bartolini, Carlo Maria [Universita Politecnica delle Marche, Dipartimento di Energetica, Via Brecce Bianche, Ancona (AN) 60100 (Italy); Brandoni, Caterina [Universita Telematica e-Campus, Ingegneria Energetica, Via Isimbardi 10, Novedrate (CO) 22060 (Italy); Feliciotti, Petro [Universita Politecnica delle Marche, Dipartimento di Ingegneria Informatica, Gestionale e dell' Automazione, Via Brecce Bianche, Ancona (AN) 60100 (Italy)

    2011-03-15

    Micro-combined heat and power (CHP) systems are a key resource to meet the EUCO{sub 2} reduction agreed in the Kyoto Protocol. In the near future they are likely to spread significantly through applications in the residential and service sectors, since they can provide considerably higher primary energy efficiencies than plants generating electricity and heat separately. A 28 kW{sub e} natural gas, automotive-derived internal combustion engine CHP system was modeled with a view to comparing constant and variable speed operation modes. Besides their energy performances, the paper addresses the major factors involved in their economic evaluation and describes a method to assess their economic feasibility. Typical residential and service sector applications were chosen as test cases and the results discussed in terms of energy performances and of profitability. They showed that interesting savings can be obtained with respect to separate generation, and that they are higher for the household application in variable speed operating conditions. In fact the plant's energy performance is greatly enhanced by the possibility, for any given power, to regulate the engine's rotational speed. From the economic viewpoint, despite the higher initial cost of the variable speed concept, the system involves a shorter pay-back period and ensures greater profit. (author)

  17. Resonant vibrations resulting from the re-engineering of a constant-speed 2-bladed turbine to a variable-speed 3-bladed turbine

    NARCIS (Netherlands)

    Fleming, P.A.; Wright, A.D.; Fingersh, L.J.; Van Wingerden, J.W.

    2011-01-01

    The CART3 (Controls Advanced Research Turbine, 3-bladed) at the National Wind Technology Center has recently been converted from a 2-bladed constant speed machine to a 3-bladed variable speed machine designed specifically for controls research. The purpose of this conversion was to develop an

  18. Variable speed induction motor operation from a 20-kHz power bus

    Science.gov (United States)

    Hansen, Irving G.

    Induction motors are recognized for their simple rugged construction to date, however, their application to variable speed or servo drives has been hampered by limitations on their control. Induction motor drives tend to be complex and to display troublesome low speed characteristics due in part to nonsinusoidal driving voltages. A technique was developed which involves direct synthesis of sinusoidal driving voltages from a high frequency power bus and independent control of frequency and voltages. Separation offrequency and voltage allows independent control of rotor and stator flux, full four-quadrant operation, and instantaneous torque control. Recent test results, current status of the technology, and proposed aerospace applications will be discussed.

  19. Power Control of Permanent Magnet Generator Based Variable Speed Wind Turbines

    DEFF Research Database (Denmark)

    Deng, Fujin; Chen, Zhe

    2009-01-01

    When the wind power accounts for a large portion of the grid, it will be required to regulate the active power and reactive power. This paper investigates a MWlevel variable speed wind turbine with a permanent magnet synchronous generator (PMSG). The power control capabilities of two kinds...... of control schemes conducted respectively on this wind turbine under two conditions, including rapid wind speed change and grids faults, are compared. The simulation study of the wind turbine system is conducted using PSCAD/EMTDC, and the results show the different power control capabilities of the two...

  20. Novel Position and Speed Estimator for PM Single Phase Brushless D.C. Motor Drives

    DEFF Research Database (Denmark)

    Lepure, Liviu I.; Andreescu, Gheorghe-Daniel; Iles, Doris

    2010-01-01

    A novel position and speed estimator for single phase permanent magnet brushless d.c. (PMBLDC) motor drives, based on flux integration and prior knowledge of ΨPM (θ) is proposed here and an adequate correction algorithm is adopted in order to increase the robustness to noise and to reduce...... the sensitivity to accuracy of flux linkage estimation. A speed and current close loop control is employed based on the Hall signal and the motor is controlled at different speeds in order to validate the proposed estimation algorithm with satisfying results. The position correction effect is analyzed...

  1. A Neural Network Controller for Variable-Speed Variable-Pitch Wind Energy Conversion Systems Using Generalized Minimum Entropy Criterion

    Directory of Open Access Journals (Sweden)

    Mifeng Ren

    2014-01-01

    Full Text Available This paper considers the neural network controller design problem for variable pitch wind energy conversion systems (WECS with non-Gaussian wind speed disturbances in the stochastic distribution control framework. The approach here is used to directly model the unknown control law based on a fixed neural network (the number of layers and nodes in a neural network is fixed without the need to construct a separate model for the WECS. In order to characterize the randomness of the WECS, a generalized minimum entropy criterion is established to train connection weights of the neural network. For the train purpose, both kernel density estimation method and sliding window technique are adopted to estimate the PDF of tracking error and entropies. Due to the unknown process dynamics, the gradient of the objective function in a gradient-descent-type algorithm is estimated using an incremental perturbation method. The proposed approach is illustrated on a simulated WECS with non-Gaussian wind speed.

  2. Variable speed limit strategies’ analysis with cell transmission model on freeway

    Science.gov (United States)

    Li, Shubin; Cao, Danni

    2017-08-01

    Mainline freeway traffic flow control is one of the primary methods of traffic management, which can present the best network situation. In this paper, we integrate variable speed limit (VSL) strategy into the cell transmission model (CTM). Then the implementation of the integrated model on freeway traffic network is discussed. A novel optimal model of controlling freeway traffic flow is proposed for minimizing the total travelling time in the network. A solution algorithm is designed by using a simulation method. Considering the main purpose of the speed limit strategy is to control the mainstream flow, we compare the case where the VSL is used with the one without VSL. A simulation is implemented to show that the control strategy is efficient in describing system’s dynamic performance and the dynamic speed limit strategy significantly alleviates congestion.

  3. Modeling and Design Optimization of Variable-Speed Wind Turbine Systems

    Directory of Open Access Journals (Sweden)

    Ulas Eminoglu

    2014-01-01

    Full Text Available As a result of the increase in energy demand and government subsidies, the usage of wind turbine system (WTS has increased dramatically. Due to the higher energy production of a variable-speed WTS as compared to a fixed-speed WTS, the demand for this type of WTS has increased. In this study, a new method for the calculation of the power output of variable-speed WTSs is proposed. The proposed model is developed from the S-type curve used for population growth, and is only a function of the rated power and rated (nominal wind speed. It has the advantage of enabling the user to calculate power output without using the rotor power coefficient. Additionally, by using the developed model, a mathematical method to calculate the value of rated wind speed in terms of turbine capacity factor and the scale parameter of the Weibull distribution for a given wind site is also proposed. Design optimization studies are performed by using the particle swarm optimization (PSO and artificial bee colony (ABC algorithms, which are applied into this type of problem for the first time. Different sites such as Northern and Mediterranean sites of Europe have been studied. Analyses for various parameters are also presented in order to evaluate the effect of rated wind speed on the design parameters and produced energy cost. Results show that proposed models are reliable and very useful for modeling and optimization of WTSs design by taking into account the wind potential of the region. Results also show that the PSO algorithm has better performance than the ABC algorithm for this type of problem.

  4. A New Fault Diagnosis Algorithm for PMSG Wind Turbine Power Converters under Variable Wind Speed Conditions

    Directory of Open Access Journals (Sweden)

    Yingning Qiu

    2016-07-01

    Full Text Available Although Permanent Magnet Synchronous Generator (PMSG wind turbines (WTs mitigate gearbox impacts, they requires high reliability of generators and converters. Statistical analysis shows that the failure rate of direct-drive PMSG wind turbines’ generators and inverters are high. Intelligent fault diagnosis algorithms to detect inverters faults is a premise for the condition monitoring system aimed at improving wind turbines’ reliability and availability. The influences of random wind speed and diversified control strategies lead to challenges for developing intelligent fault diagnosis algorithms for converters. This paper studies open-circuit fault features of wind turbine converters in variable wind speed situations through systematic simulation and experiment. A new fault diagnosis algorithm named Wind Speed Based Normalized Current Trajectory is proposed and used to accurately detect and locate faulted IGBT in the circuit arms. It is compared to direct current monitoring and current vector trajectory pattern approaches. The results show that the proposed method has advantages in the accuracy of fault diagnosis and has superior anti-noise capability in variable wind speed situations. The impact of the control strategy is also identified. Experimental results demonstrate its applicability on practical WT condition monitoring system which is used to improve wind turbine reliability and reduce their maintenance cost.

  5. Feasibility of a Simple Small Wind Turbine with Variable-Speed Regulation Made of Commercial Components

    Directory of Open Access Journals (Sweden)

    Jesús Peláez Vara

    2013-07-01

    Full Text Available The aim of this study was to propose and evaluate a very small wind turbine (VSWT that competes with commercial grid-connected VSWTs in terms of simplicity, robustness and price. Its main components are a squirrel-cage induction generator (SCIG driven by a frequency converter. The system has a direct-drive shaft, and may be constructed with commercial equipment. Simulation of the wind turbine effect is done with a motor. A control program regulates the variable-speed of rotation through three operational modes: (i to drive the turbine to its optimum operation point; (ii to limit its maximum rotational speed; and (iii to limit the maximum power it generates. Two tests were performed, in order to evaluate the dynamic response of this system under variable wind speeds. The tests demonstrate that the system operates at the optimum operational point of the turbine, and within the set limits of maximum rotational speed and maximum generated power. The drop in performance in relation to its nominal value is about 75%, when operating at 50% of the nominal power. In summary, this VSWT with its proposed control program is feasible and reliable for operating direct-shaft grid-connected VSWTs.

  6. Variable Speed Wind Turbine Based on Multiple Generators Drive-Train Configuration

    DEFF Research Database (Denmark)

    Deng, Fujin; Chen, Zhe

    2010-01-01

    A variable speed wind turbine is presented in this paper, where multiple permanent magnet synchronous generators (MPMSGs) drive-train configuration is employed in the wind turbine. A cascaded multilevel converter interface based on the MPMSGs is developed to synthesize a desired high ac sinusoidal...... reduce the fluctuation of the electromagnetic torque sum and results in a good performance for the MPMSGs structure. The simulation study is conducted using PSCAD/EMTDC, and the results verify the feasibility of this variable speed wind turbine based on multiple generators drive-train configuration....... output voltage, which could be directly connected to the grids. What is more, such arrangement has been made so that the output ac voltage having a selected phase angle difference among the stator windings of multiple generators. A phase angle shift strategy is proposed in this paper, which effectively...

  7. A New Structure Based on Cascaded Multilevel Converter for Variable Speed Wind Turbine

    DEFF Research Database (Denmark)

    Deng, Fujin; Chen, Zhe

    2010-01-01

    An alternative structure for variable speed wind turbine, using multiple permanent magnet synchronous generators (PMSGs) drive-train configuration and cascaded multilevel converter is proposed in this paper. This study presents a power electronic solution for the wind turbine. A transformer......-less cascaded multilevel converter interface based on PMSGs is developed to synthesize a desired high ac sinusoidal output voltage. The benefits of high power and high ac voltage make this structure possible to be applied in the wind power generation. In addition, the bulky transformer could be omitted....... A simulation model of 10 MW variable speed wind turbine based on PMSGs developed in PSCAD/EMTDC is presented. The dynamic performance of grid-connected wind turbine is analyzed. Simulation results shows that the proposed structure may be attractive in wind power generation....

  8. Modelling and control algorithms of the cross conveyors line with multiengine variable speed drives

    Science.gov (United States)

    Cheremushkina, M. S.; Baburin, S. V.

    2017-02-01

    The paper deals with the actual problem of developing the control algorithm that meets the technical requirements of the mine belt conveyors, and enables energy and resource savings taking into account a random sort of traffic. The most effective method of solution of these tasks is the construction of control systems with the use of variable speed drives for asynchronous motors. The authors designed the mathematical model of the system ‘variable speed multiengine drive - conveyor - control system of conveyors’ that takes into account the dynamic processes occurring in the elements of the transport system, provides an assessment of the energy efficiency of application the developed algorithms, which allows one to reduce the dynamic overload in the belt to 15-20%.

  9. Perfection of badminton players’ speed-power fitness with the help of training means’ variable modules

    Directory of Open Access Journals (Sweden)

    I.V. Karatnyk

    2016-06-01

    Full Text Available Purpose: to determine effectiveness of badminton players’ speed power fitness program’s perfection at stage of specialized basic training with different variants of training means modules’ combination. Material: in experiment badminton players of 15-17 years’ age (from 1st sports grade to master of sports participated. The sportsmen were divided into three experimental groups (10 persons in each. The trainings were being conducted during 24 weeks by different variants of program. Results: we created different complexes of exercises, combined in three modules (every of each lasted eight week micro-cycles. Every module has more expressed meaningful parts (1 – speed, 2 – power, 3 – jumping. All modules were combined in program of badminton players’ speed power fitness perfection. For every experimental group we worked out distinguishing variant of modules’ combination in program (first variant – 1-2-3 modules; second – 2-3-1; third – 3-1-2. General duration of program was 24 week micro-cycles. Conclusions: we recommended some variants of variable modules’ combination for badminton players’ speed-power fitness perfection. With it, we can regard total influence on the following: speed-power endurance, work with support on own body, quick movements of different body links.

  10. Perfection of badminton players’ speed-power fitness with the help of training means’ variable modules

    Directory of Open Access Journals (Sweden)

    Karatnyk I.V.

    2016-06-01

    Full Text Available Purpose: to determine effectiveness of badminton players’ speed power fitness program’s perfection at stage of specialized basic training with different variants of training means modules’ combination. Material: in experiment badminton players of 15-17 years’ age (from 1st sports grade to master of sports participated. The sportsmen were divided into three experimental groups (10 persons in each. The trainings were being conducted during 24 weeks by different variants of program. Results: we created different complexes of exercises, combined in three modules (every of each lasted eight week micro-cycles. Every module has more expressed meaningful parts (1 - speed, 2 - power, 3 - jumping. All modules were combined in program of badminton players’ speed power fitness perfection. For every experimental group we worked out distinguishing variant of modules’ combination in program (first variant - 1-2-3 modules; second - 2-3-1; third - 3-1-2. General duration of program was 24 week micro-cycles. Conclusions: we recommended some variants of variable modules’ combination for badminton players’ speed-power fitness perfection. With it, we can regard total influence on the following: speed-power endurance, work with support on own body, quick movements of different body links.

  11. Variable speed actuators enhance SBR wastewater treatment; Drehzahlveraenderbare Stellantriebe optimieren SBR-Abwasserreinigungsverfahren

    Energy Technology Data Exchange (ETDEWEB)

    Koegel, Ottmar; Koehler, Steffen [SIPOS Aktorik GmbH, Altdorf (Germany)

    2008-03-15

    Sequencing batch reactors (SBRs) became established as an effective option to conventional activated sludge systems at wastewater treatment plants. Today, the SBR's ''fill-and-draw'' process of treating and discharging undesirable components had been widely adopted by the municipal and industrial sector. In this article the Intermittent Cyclic Extended Aeration System (ICEAS) approach to SBR technology developed by ITT Sanitaire is reviewed. The contribution of variable speed actuation technology from SIPOS Aktorik is outlined. (orig.)

  12. Variable-speed wind power system with improved energy capture via multilevel conversion

    Science.gov (United States)

    Erickson, Robert W.; Al-Naseem, Osama A.; Fingersh, Lee Jay

    2005-05-31

    A system and method for efficiently capturing electrical energy from a variable-speed generator are disclosed. The system includes a matrix converter using full-bridge, multilevel switch cells, in which semiconductor devices are clamped to a known constant DC voltage of a capacitor. The multilevel matrix converter is capable of generating multilevel voltage wave waveform of arbitrary magnitude and frequencies. The matrix converter can be controlled by using space vector modulation.

  13. Improving Fault Ride-Through Capability of Variable Speed Wind Turbines in Distribution Networks

    DEFF Research Database (Denmark)

    Mokryani, Geev; Siano, P.; Piccolo, Antonio

    2013-01-01

    In this paper, a fuzzy controller for improving the fault ride-through (FRT) capability of variable speed wind turbines (WTs) equipped with a doubly fed induction generator (DFIG) is presented. DFIGs can be used as reactive power sources to control the voltage at the point of common coupling (PCC...... on a real 37-bus weak distribution system confirmed that the proposed controller can enhance the FRT capability....

  14. On the Profitability of Variable Speed Pump-Storage-Power in Frequency Restoration Reserve

    Science.gov (United States)

    Filipe, Jorge; Bessa, Ricardo; Moreira, Carlos; Silva, Bernardo

    2017-04-01

    The increase penetration of renewable energy sources (RES) into the European power system has introduced a significant amount of variability and uncertainty in the generation profiles raising the needs for ancillary services as well as other tools like demand response, improved generation forecasting techniques and changes to the market design. While RES is able to replace energy produced by the traditional centralized generation, it cannot displace its capacity in terms of ancillary services provided. Therefore, centralized generation capacity must be retained to perform this function leading to over-capacity issues and underutilisation of the assets. Large-scale reversible hydro power plants represent the majority of the storage solution installed in the power system. This technology comes with high investments costs, hence the constant search for methods to increase and diversify the sources of revenue. Traditional fixed speed pump storage units typically operate in the day-ahead market to perform price arbitrage and, in some specific cases, provide downward replacement reserve (RR). Variable speed pump storage can not only participate in RR but also contribute to FRR, given their ability to control its operating point in pumping mode. This work does an extended analysis of a complete bidding strategy for Pumped Storage Power, enhancing the economic advantages of variable speed pump units in comparison with fixed ones.

  15. High-speed limnology: using advanced sensors to investigate spatial variability in biogeochemistry and hydrology.

    Science.gov (United States)

    Crawford, John T; Loken, Luke C; Casson, Nora J; Smith, Colin; Stone, Amanda G; Winslow, Luke A

    2015-01-06

    Advanced sensor technology is widely used in aquatic monitoring and research. Most applications focus on temporal variability, whereas spatial variability has been challenging to document. We assess the capability of water chemistry sensors embedded in a high-speed water intake system to document spatial variability. This new sensor platform continuously samples surface water at a range of speeds (0 to >45 km h(-1)) resulting in high-density, mesoscale spatial data. These novel observations reveal previously unknown variability in physical, chemical, and biological factors in streams, rivers, and lakes. By combining multiple sensors into one platform, we were able to detect terrestrial-aquatic hydrologic connections in a small dystrophic lake, to infer the role of main-channel vs backwater nutrient processing in a large river and to detect sharp chemical changes across aquatic ecosystem boundaries in a stream/lake complex. Spatial sensor data were verified in our examples by comparing with standard lab-based measurements of selected variables. Spatial fDOM data showed strong correlation with wet chemistry measurements of DOC, and optical NO3 concentrations were highly correlated with lab-based measurements. High-frequency spatial data similar to our examples could be used to further understand aquatic biogeochemical fluxes, ecological patterns, and ecosystem processes, and will both inform and benefit from fixed-site data.

  16. High-speed limnology: Using advanced sensors to investigate spatial variability in biogeochemistry and hydrology

    Science.gov (United States)

    Crawford, John T.; Loken, Luke C.; Casson, Nora J.; Smith, Collin; Stone, Amanda G.; Winslow, Luke A.

    2015-01-01

    Advanced sensor technology is widely used in aquatic monitoring and research. Most applications focus on temporal variability, whereas spatial variability has been challenging to document. We assess the capability of water chemistry sensors embedded in a high-speed water intake system to document spatial variability. This new sensor platform continuously samples surface water at a range of speeds (0 to >45 km h–1) resulting in high-density, mesoscale spatial data. These novel observations reveal previously unknown variability in physical, chemical, and biological factors in streams, rivers, and lakes. By combining multiple sensors into one platform, we were able to detect terrestrial–aquatic hydrologic connections in a small dystrophic lake, to infer the role of main-channel vs backwater nutrient processing in a large river and to detect sharp chemical changes across aquatic ecosystem boundaries in a stream/lake complex. Spatial sensor data were verified in our examples by comparing with standard lab-based measurements of selected variables. Spatial fDOM data showed strong correlation with wet chemistry measurements of DOC, and optical NO3 concentrations were highly correlated with lab-based measurements. High-frequency spatial data similar to our examples could be used to further understand aquatic biogeochemical fluxes, ecological patterns, and ecosystem processes, and will both inform and benefit from fixed-site data.

  17. Power maximization of variable-speed variable-pitch wind turbines using passive adaptive neural fault tolerant control

    Science.gov (United States)

    Habibi, Hamed; Rahimi Nohooji, Hamed; Howard, Ian

    2017-09-01

    Power maximization has always been a practical consideration in wind turbines. The question of how to address optimal power capture, especially when the system dynamics are nonlinear and the actuators are subject to unknown faults, is significant. This paper studies the control methodology for variable-speed variable-pitch wind turbines including the effects of uncertain nonlinear dynamics, system fault uncertainties, and unknown external disturbances. The nonlinear model of the wind turbine is presented, and the problem of maximizing extracted energy is formulated by designing the optimal desired states. With the known system, a model-based nonlinear controller is designed; then, to handle uncertainties, the unknown nonlinearities of the wind turbine are estimated by utilizing radial basis function neural networks. The adaptive neural fault tolerant control is designed passively to be robust on model uncertainties, disturbances including wind speed and model noises, and completely unknown actuator faults including generator torque and pitch actuator torque. The Lyapunov direct method is employed to prove that the closed-loop system is uniformly bounded. Simulation studies are performed to verify the effectiveness of the proposed method.

  18. Production of a phage-displayed single chain variable fragment ...

    African Journals Online (AJOL)

    Purpose: To develop specific single chain variable fragments (scFv) against infectious bursal disease virus (IBDV) via phage display technology. Methods: Purified viruses were initially applied for iterative panning rounds of scFv phage display libraries. The binding ability of the selected scFv antibody fragments against the ...

  19. Modeling the Power Variability of Core Speed Scaling on Homogeneous Multicore Systems

    Directory of Open Access Journals (Sweden)

    Zhihui Du

    2017-01-01

    Full Text Available We describe a family of power models that can capture the nonuniform power effects of speed scaling among homogeneous cores on multicore processors. These models depart from traditional ones, which assume that individual cores contribute to power consumption as independent entities. In our approach, we remove this independence assumption and employ statistical variables of core speed (average speed and the dispersion of the core speeds to capture the comprehensive heterogeneous impact of subtle interactions among the underlying hardware. We systematically explore the model family, deriving basic and refined models that give progressively better fits, and analyze them in detail. The proposed methodology provides an easy way to build power models to reflect the realistic workings of current multicore processors more accurately. Moreover, unlike the existing lower-level power models that require knowledge of microarchitectural details of the CPU cores and the last level cache to capture core interdependency, ours are easier to use and scalable to emerging and future multicore architectures with more cores. These attributes make the models particularly useful to system users or algorithm designers who need a quick way to estimate power consumption. We evaluate the family of models on contemporary x86 multicore processors using the SPEC2006 benchmarks. Our best model yields an average predicted error as low as 5%.

  20. Variable population exposure and distributed travel speeds in least-cost tsunami evacuation modelling

    Science.gov (United States)

    Fraser, Stuart A.; Wood, Nathan J.; Johnston, David A.; Leonard, Graham S.; Greening, Paul D.; Rossetto, Tiziana

    2014-01-01

    Evacuation of the population from a tsunami hazard zone is vital to reduce life-loss due to inundation. Geospatial least-cost distance modelling provides one approach to assessing tsunami evacuation potential. Previous models have generally used two static exposure scenarios and fixed travel speeds to represent population movement. Some analyses have assumed immediate departure or a common evacuation departure time for all exposed population. Here, a method is proposed to incorporate time-variable exposure, distributed travel speeds, and uncertain evacuation departure time into an existing anisotropic least-cost path distance framework. The method is demonstrated for hypothetical local-source tsunami evacuation in Napier City, Hawke's Bay, New Zealand. There is significant diurnal variation in pedestrian evacuation potential at the suburb level, although the total number of people unable to evacuate is stable across all scenarios. Whilst some fixed travel speeds approximate a distributed speed approach, others may overestimate evacuation potential. The impact of evacuation departure time is a significant contributor to total evacuation time. This method improves least-cost modelling of evacuation dynamics for evacuation planning, casualty modelling, and development of emergency response training scenarios. However, it requires detailed exposure data, which may preclude its use in many situations.

  1. Variable Speed Limits: Strategies to Improve Safety and Traffic Parameters for a Bottleneck

    Directory of Open Access Journals (Sweden)

    M. Z. Hasanpour

    2017-04-01

    Full Text Available The primary purpose of the speed limit system is to enforce reasonable and safe speed. To reduce secondary problems such as accidents and queuing, Variable Speed Limits (VSL has been suggested. In this paper VSL is used to better safety and traffic parameters. Traffic parameters including speed, queue length and stopping time have been pondering. For VLS, an optimization decision tree algorithm with the function of microscopic simulation was used. The results in case of sub saturated, saturated and supersaturated at a bottleneck are examined and compared with the Allaby logic tree. The results show that the proposed decision tree shows an improved performance in terms of safety and comfort along the highway. The VSL pilot project is part of the Road Safety Improvement Program included in Iran’s road safety action plan that is in the research process in the BHRC Research Institute, Road and Housing & Urban Development Research that is planned for next 10-year Transportation safety view Plan.

  2. A hybrid approach to fault diagnosis of roller bearings under variable speed conditions

    Science.gov (United States)

    Wang, Yanxue; Yang, Lin; Xiang, Jiawei; Yang, Jianwei; He, Shuilong

    2017-12-01

    Rolling element bearings are one of the main elements in rotating machines, whose failure may lead to a fatal breakdown and significant economic losses. Conventional vibration-based diagnostic methods are based on the stationary assumption, thus they are not applicable to the diagnosis of bearings working under varying speeds. This constraint limits the bearing diagnosis to the industrial application significantly. A hybrid approach to fault diagnosis of roller bearings under variable speed conditions is proposed in this work, based on computed order tracking (COT) and variational mode decomposition (VMD)-based time frequency representation (VTFR). COT is utilized to resample the non-stationary vibration signal in the angular domain, while VMD is used to decompose the resampled signal into a number of band-limited intrinsic mode functions (BLIMFs). A VTFR is then constructed based on the estimated instantaneous frequency and instantaneous amplitude of each BLIMF. Moreover, the Gini index and time-frequency kurtosis are both proposed to quantitatively measure the sparsity and concentration measurement of time-frequency representation, respectively. The effectiveness of the VTFR for extracting nonlinear components has been verified by a bat signal. Results of this numerical simulation also show the sparsity and concentration of the VTFR are better than those of short-time Fourier transform, continuous wavelet transform, Hilbert–Huang transform and Wigner–Ville distribution techniques. Several experimental results have further demonstrated that the proposed method can well detect bearing faults under variable speed conditions.

  3. Improving voltage stability by utilizing reactive power injection capability of variable speed wind turbines

    Energy Technology Data Exchange (ETDEWEB)

    Ullah, N.R.; Thiringer, T. [Chalmer Univ. of Technology, Goteborg (Sweden). Dept. of Energy and Environment

    2008-07-01

    The effect of wind power generation on voltage stability was investigated by studying various reactive power control algorithms in combination with different wind power systems. The impact of reactive power injection by the systems on steady-state power transfer limits was analyzed. Critical load and wind generation situations were also determined. A power system model based on the Swedish transmission system was used. The wind farm installation was connected to ta 400 kV transmission grid. Four different wind turbine systems were considered: (1) traditional fixed speed; (2) variable speed wind turbine producing active power at unity power factor; (3) a variable speed turbine with a full power grid side converter; and (4) a wind turbine system with a large grid side converter. Simulations were conducted to consider the tap changing action of the transformer for all 4 types of wind turbine. Results of the study showed that use of the reactive power injection capability of wind turbine systems can increase the grid voltage stability limit. The wind farm also assisted in delaying voltage collapse events and prevented the transformer's tap from operating. It was concluded that the use of grid side converters substantially improved maximum deliverable loads. 14 refs., 2 tabs., 15 figs.

  4. Design and Analysis of an Axially Laminated Reluctance Motor for Variable-Speed Applications

    Directory of Open Access Journals (Sweden)

    BESER, E. K.

    2013-02-01

    Full Text Available In this paper, an axially laminated reluctance motor is presented. First, a set of a finite element analysis (FEA on three different axially laminated rotor geometries was carried out and torque profiles of the rotors were predicted. The effect of the stator slot skewing on the torque profiles were also examined in the analysis. After deciding the rotor geometry, the mathematical model of the proposed motor was formed in terms of a,b,c variables and simulations were performed. Motor prototype and motor drive were introduced. Torque profiles of the motor were measured for different current values and load test were realized. Experimental results were compared to analysis and simulation results. There is a good accordance between experimental and simulation results. When the proposed motor is operated with electrical 120? mode as a brushless DC motor, the torque versus speed characteristic shows a DC series motor characteristic and speed of the motor can be easily controlled by regulating the bus voltage. These features make the proposed motor convenient for variable-speed applications such as electrical vehicles.

  5. Mathematical Model of Variable Speed Refrigerant Compressor to Predict its Transient Behavior

    Science.gov (United States)

    Yanagisawa, Tadashi; Shimizu, Takashi; Fukuta, Mitsuhiro; Ikeda, Kiyoharu

    To predict performance of a refrigerating cycle it is effective to use a computer simulation program which employs mathematical models of the cycle components. This paper describes a mathematical model of a rotary compressor which can expersses transient behavior of the compressor with variable operting speed. The compressor is divided into two control volumes and two control bodies, and the model is expressed by six governing differential equations on specific enthalpies of refrigerant in the control volumes, mass flow rates of refrigerant delivered from the volumes and temperatures of the bodies. Simulation results and experimental data are favorably compared for transient behaviors of the compressor under wide operating speed range. The model developed will be effectively integrated into cycle simulation of dynamic responses of inverter driven heat pumps.

  6. Variable speed wind turbine control by discrete-time sliding mode approach.

    Science.gov (United States)

    Torchani, Borhen; Sellami, Anis; Garcia, Germain

    2016-05-01

    The aim of this paper is to propose a new design variable speed wind turbine control by discrete-time sliding mode approach. This methodology is designed for linear saturated system. The saturation constraint is reported on inputs vector. To this end, the back stepping design procedure is followed to construct a suitable sliding manifold that guarantees the attainment of a stabilization control objective. It is well known that the mechanisms are investigated in term of the most proposed assumptions to deal with the damping, shaft stiffness and inertia effect of the gear. The objectives are to synthesize robust controllers that maximize the energy extracted from wind, while reducing mechanical loads and rotor speed tracking combined with an electromagnetic torque. Simulation results of the proposed scheme are presented. Copyright © 2016 ISA. Published by Elsevier Ltd. All rights reserved.

  7. Control Method for Variable Speed Wind Turbines to Support Temporary Primary Frequency Control

    DEFF Research Database (Denmark)

    Wang, Haijiao; Chen, Zhe; Jiang, Quanyuan

    2014-01-01

    This paper develops a control method for variable speed wind turbines (VSWTs) to support temporary primary frequency control of power system. The control method contains two parts: (1) up-regulate support control when a frequency drop event occurs; (2) down-regulate support control when a frequency...... rise event occurs. The up-regulate support is achieved by adaptively utilizing the wind energy curtailed by the pitch control and the kinetic energy stored in the rotating mass of the turbine blades. The down-regulate support is achieved by the pitch control. Furthermore, the up- and down......-regulate capabilities are quantified under different wind speeds. Finally, the whole control method is verified in a test power system established in MATLAB/Simulink, which contains a wind farm of 180 VSWTs....

  8. Dual stator winding variable speed asynchronous generator: magnetic equivalent circuit with saturation, FEM analysis and experiments

    Science.gov (United States)

    Tutelea, L. N.; Muntean, N.; Deaconu, S. I.; Cunţan, C. D.

    2016-02-01

    The authors carried out a theoretical and experimental study of dual stator winding squirrel cage asynchronous generator (DSWA) behaviour in the presence of saturation regime (non-sinusoidal) due to the variable speed operation. The main aims are the determination of the relations of calculating the equivalent parameters of the machine windings, FEM validation of parameters and characteristics with free FEMM 4.2 computing software and the practice experimental tests for verifying them. Issue is limited to three phase range of double stator winding cage-asynchronous generator of small sized powers, the most currently used in the small adjustable speed wind or hydro power plants. The tests were carried out using three-phase asynchronous generator having rated power of 6 [kVA].

  9. An adaptive feedback linearization strategy for variable speed wind energy conversion systems

    Energy Technology Data Exchange (ETDEWEB)

    Valenciaga, F.; Puleston, P.F.; Battaiotto, P.E.; Mantz, R.J. [Universidad Nacional de La Plata, Depto. de Electrotecnia, La Plata (Argentina)

    2000-07-01

    This paper presents a control strategy based on adaptive feedback linearization intended for variable speed grid-connected wind energy conversion systems (WECS). The proposed adaptive control law accomplishes energy capture maximization by tracking the wind speed fluctuations. In addition, it linearizes the system even in the presence of turbine model uncertainties, allowing the closed-loop dynamic behaviour to be determined by a simple tuning of the controller parameters. Particularly, the attention is focused on WECS with slip power recovery, which use a power conversion stage as a rotor-controlled double-output induction generator. However, the concepts behind the proposed control strategy are general and can be easily extended to other WECS configurations. (Author)

  10. Speed and Cardiac Recovery Variables Predict the Probability of Elimination in Equine Endurance Events.

    Directory of Open Access Journals (Sweden)

    Mohamed Younes

    Full Text Available Nearly 50% of the horses participating in endurance events are eliminated at a veterinary examination (a vet gate. Detecting unfit horses before a health problem occurs and treatment is required is a challenge for veterinarians but is essential for improving equine welfare. We hypothesized that it would be possible to detect unfit horses earlier in the event by measuring heart rate recovery variables. Hence, the objective of the present study was to compute logistic regressions of heart rate, cardiac recovery time and average speed data recorded at the previous vet gate (n-1 and thus predict the probability of elimination during successive phases (n and following in endurance events. Speed and heart rate data were extracted from an electronic database of endurance events (80-160 km in length organized in four countries. Overall, 39% of the horses that started an event were eliminated--mostly due to lameness (64% or metabolic disorders (15%. For each vet gate, logistic regressions of explanatory variables (average speed, cardiac recovery time and heart rate measured at the previous vet gate and categorical variables (age and/or event distance were computed to estimate the probability of elimination. The predictive logistic regressions for vet gates 2 to 5 correctly classified between 62% and 86% of the eliminated horses. The robustness of these results was confirmed by high areas under the receiving operating characteristic curves (0.68-0.84. Overall, a horse has a 70% chance of being eliminated at the next gate if its cardiac recovery time is longer than 11 min at vet gate 1 or 2, or longer than 13 min at vet gates 3 or 4. Heart rate recovery and average speed variables measured at the previous vet gate(s enabled us to predict elimination at the following vet gate. These variables should be checked at each veterinary examination, in order to detect unfit horses as early as possible. Our predictive method may help to improve equine welfare and

  11. A Comparison Study between Two MPPT Control Methods for a Large Variable-Speed Wind Turbine under Different Wind Speed Characteristics

    Directory of Open Access Journals (Sweden)

    Dongran Song

    2017-05-01

    Full Text Available Variable speed wind turbines (VSWTs usually adopt a maximum power point tracking (MPPT method to optimize energy capture performance. Nevertheless, obtained performance offered by different MPPT methods may be affected by the impact of wind turbine (WT’s inertia and wind speed characteristics and it needs to be clarified. In this paper, the tip speed ratio (TSR and optimal torque (OT methods are investigated in terms of their performance under different wind speed characteristics on a 1.5 MW wind turbine model. To this end, the TSR control method based on an effective wind speed estimator and the OT control method are firstly presented. Then, their performance is investigated and compared through simulation test results under different wind speeds using Bladed software. Comparison results show that the TSR control method can capture slightly more wind energy at the cost of high component loads than the other one under all wind conditions. Furthermore, it is found that both control methods present similar trends of power reduction that is relevant to mean wind speed and turbulence intensity. From the obtained results, we demonstrate that, to further improve MPPT capability of large VSWTs, other advanced control methods using wind speed prediction information need to be addressed.

  12. Comparison of simulators for variable-speed wind turbine transient analysis

    DEFF Research Database (Denmark)

    Seman, S.; Iov, Florin; Niiranen, J.

    2006-01-01

    This paper presents a comparison of three variable-speed wind turbine simulators used for a 2 MW wind turbine short-term transient behaviour study during a symmetrical network disturbance. The simulator with doubly fed induction generator (DFIG) analytical model, the simulator with a finite element...... method (FEM) DFIG model and the wind turbine simulator with an analytical model of DFIG are compared. The comparison of the simulation results shows the influence of the different modelling approaches on the short-term transient simulation accuracy...

  13. A Fuzzy Logic Controller to Increase Fault Ride-Through Capability of Variable Speed Wind Turbines

    Directory of Open Access Journals (Sweden)

    Geev Mokryani

    2012-01-01

    Full Text Available A fuzzy controller for improving Fault Ride-Through (FRT capability of Variable Speed Wind Turbines (WTs equipped with Doubly Fed Induction Generator (DFIG is presented. The controller is designed in order to compensate the voltage at the Point of Common Coupling (PCC by regulating the reactive and active power generated by WTs. The performances of the controller are evaluated in some case studies considering a different number of wind farms in different locations. Simulations, carried out on a real 37-bus Italian weak distribution system, confirmed that the proposed controller can enhance the FRT capability in many cases.

  14. Variable frequency inverter for ac induction motors with torque, speed and braking control

    Science.gov (United States)

    Nola, F. J. (Inventor)

    1975-01-01

    A variable frequency inverter was designed for driving an ac induction motor which varies the frequency and voltage to the motor windings in response to varying torque requirements for the motor so that the applied voltage amplitude and frequency are of optimal value for any motor load and speed requirement. The slip frequency of the motor is caused to vary proportionally to the torque and feedback is provided so that the most efficient operating voltage is applied to the motor. Winding current surge is limited and a controlled negative slip causes motor braking and return of load energy to a dc power source.

  15. Second order sliding power control for a variable speed-constant frequency energy conversion system

    Energy Technology Data Exchange (ETDEWEB)

    Valenciaga, Fernando, E-mail: fval@ing.unlp.edu.a [CONICET, Laboratorio de Electronica Industrial Control e Instrumentacion (LEICI), Facultad de Ingenieria, Universidad Nacional de La Plata, CC.91, C.P. 1900 La Plata (Argentina)

    2010-12-15

    This paper presents a decoupled active and reactive power control for a variable speed-constant frequency generation system based on a brushless doubly fed reluctance machine. The control design is approached using multi-input second order sliding techniques which are specially appropriate to deal with nonlinear system models in presence of external disturbances and model inaccuracies. The controller synthesized through this theoretical framework presents very good robustness features, a finite reaching time and a chattering-free behavior. The performance of the closed loop system is assessed through representative computer simulations.

  16. Strategy for the Operation of Cooling Towers with variable Speed Fans

    CERN Document Server

    Iñigo-Golfín, J

    2001-01-01

    Within the SPS Cooling Water Project at CERN aimed at the reduction of water consumption, this primary open cooling loop will be closed and all the primary cooling circuit components will be upgraded to the new required duty and brought to the necessary safety and operability standards. In particular the tower fans will be fitted with variable frequency drives to replace the existing two speed motors. This paper presents a study to optimize the operation of SPS cooling towers taking into account outdoor conditions (wet and dry bulb temperatures) and the entirety of the primary circuit in which they will operate.

  17. Production of a phage-displayed single chain variable fragment ...

    African Journals Online (AJOL)

    Purpose: To develop specific single chain variable fragments (scFv) against infectious bursal disease ... application of vitamins as well as antibiotics can .... D in. 4. 0. 5 n m. Clone number. 1 4 7 10 13 16 19 22 25 28 31 34 37 40 43 46 49 52 55 58 61 64 67 70 73 76 79 82 85 88 91 94. Figure 1: Indirect ELISA analysis for ...

  18. Non-Linear and Linear Model Based Controller Design for Variable-Speed Wind Turbines

    Energy Technology Data Exchange (ETDEWEB)

    Hand, M. M. (National Renewable Energy Laboratory); Balas, M. J. (Department of Aerospace Engineering Sciences, University of Colorado)

    1999-04-07

    Variable-speed, horizontal axis wind turbines use blade-pitch control to meet specified objectives for three regions of operation. This paper focuses on controller design for the constant power production regime. A simple, rigid, non-linear turbine model was used to systematically perform trade-off studies between two performance metrics. Minimization of both the deviation of the rotor speed from the desired speed and the motion of the actuator is obtained through systematic selection of proportional-integral-derivative controller gain values. The gain design is performed using a non-linear turbine model and two linear models. The linear models differ only in selection of linearization point. The gain combinations resulting from design based upon each of the three models are similar. Performance under each of the three gain combinations is acceptable according to the metrics selected. The importance of operating point selection for linear models is illustrated. Because the simulation runs efficiently, the non-linear model provides the best gain design, but careful selection of the linearization point can produce acceptable gain designs from linear models.

  19. Impact of the vibrations on the environment caused by passages of trains at variable speed

    Directory of Open Access Journals (Sweden)

    Kożuch Barbara

    2016-01-01

    Full Text Available The paper deals with negative environmental impact caused by the passages of different kinds of trains at variable speed. The study is based on the measurement results which took place in Poland in 2013 on the railway line no. 4. The effect of the traction unit – Pendolino (EMU 250 on the vibration climate was analysed. The impact of passages of new trains was compared to currently operated rolling stock. The speed of trains was varying between 40 and 250 km/h. Vibration measurements were conducted by stuff of an accredited Laboratory of Structural Mechanics at Cracow University of Technology (Accreditation No. AB 826. The influence of the indicated vibrations due to passages of the trains on the building in the neighbourhood of the line was investigated. The vibration assessment was done for horizontal components of vibrations according to Polish standard code. Assessment of environmental impact was presented by indicator of perceptibility of vibration through construction (WODB, which refers to the Scales of Dynamic Influences (SDI scales. The limits specified by standards in any of the passages have not been exceeded. The change of speed or rolling stock resulted in a change in the characteristic of the vibration spectrum.

  20. Development of a variable speed limit strategy to reduce secondary collision risks during inclement weathers.

    Science.gov (United States)

    Li, Zhibin; Li, Ye; Liu, Pan; Wang, Wei; Xu, Chengcheng

    2014-11-01

    Inclement weather reduces traveler's sight distance and increases vehicle's stopping distance. Once a collision occurred during inclement weather and resulted in a slow traffic, approaching vehicles may not have adequate time to make emergency responses to the hazardous traffic, resulting in increased potentials of secondary collisions. The primary objective of this study is to develop a control strategy of variable speed limits (VSL) to reduce the risks of secondary collisions during inclement weathers. By analyzing the occurrence condition of secondary collision, the VSL strategy is proposed to dynamically adjust the speed limits according to the current traffic and weather conditions. A car-following model is modified to simulate the vehicle maneuvers with the VSL control. Two surrogate safety measures, based on the time-to-collision notion, are used to evaluate the control effects of VSL. Five weather scenarios are evaluated in simulation. The results show that the VSL strategy effectively reduces the risks of secondary collisions in various weather types. The time exposed time-to-collision (TET) is reduced by 41.45%-50.74%, and the time integrated time-to-collision (TIT) is reduced by 38.19%-41.19%. The safety effects are compared to those with a previous VSL strategy. The results show that in most cases our strategy outperforms the previous one. We also evaluate how driver's compliance to speed limit affects the effectiveness of VSL control. Copyright © 2014 Elsevier Ltd. All rights reserved.

  1. Analysis And Synthesis Of Model Reference Controller For Variable Speed Wind Generators Inertial Support

    Science.gov (United States)

    Bećirović, Elvisa; Osmić, Jakub; Kušljugić, Mirza; Perić, Nedjeljko

    2015-01-01

    Model Reference Controller (MRC) for contribution of Variable Speed Wind Generators (VSWG) in inertial response of Electrical Power System (EPS) is presented and analyzed in this paper. MRC is synthesized based on a model of Generating Unit With non-Reheat Steam Turbine (GUNRST) thus enabling VSWG to emulate GUNRST response during the initial stage of dynamic frequency response ie inertial phase. Very important property of conventional steam generating units is that its contribution to inertial phase response is independent from the initial generating power. By using MRC in VSWG it is accomplished that in most common wind speed region (3-12 m/s) VSWG inertial support is almost independent from wind speed. Since in most EPSs VSWG replaces conventional steam generators, application of MRC algorithm provides that the characteristics of EPS in terms of inertial response are preserved, regardless of the growing trend of introducing VSWG. Evaluation analysis of the proposed MRC is performed on modified nine bus power system when VSWG with MRC is connected to one of the power system buses.

  2. HIGH-SPEED SINGLE QUANTUM DOT IMAGING OF IN LIVE CELLS REVEAL HOP DIFFUSION

    DEFF Research Database (Denmark)

    Lagerholm, B. Christoffer; Clausen, Mathias P.

    2011-01-01

    Ultra high-speed single particle tracking (image frame rates 40-50 kHz) experiments with 40 nm gold particles has indicated that lipids and proteins in the plasma membrane undergo hop-diffusion between nanometer sized compartments (Fujiwara et al. (2002) J Cell Biol. 157:1071-81). These findings...... have yet to be independently confirmed. In this work, we show that high-speed single particle tracking with quantum dots (QDs) and using a standard wide-field fluorescence microscope and an EMCCD is possible at image acquisition rates of up to ~2000 Hz. The spatial precision in these experiments is ~40...... nm (as determined from the standard deviation of repeated position measurements of an immobile QD on a cell). Using this system, we show that membrane proteins and lipids, which have been exogenously labeled with functionalized QDs, show examples of three types of motion in the plasma membrane...

  3. High-speed atomic force microscopy: Structure and dynamics of single proteins.

    Science.gov (United States)

    Casuso, Ignacio; Rico, Felix; Scheuring, Simon

    2011-10-01

    For surface analysis of biological molecules, atomic force microscopy (AFM) is an appealing technique combining data acquisition under physiological conditions, for example buffer solution, room temperature and ambient pressure, and high resolution. However, a key feature of life, dynamics, could not be assessed until recently because of the slowness of conventional AFM setups. Thus, for observing bio-molecular processes, the gain of image acquisition speed signifies a key progress. Here, we review the development and recent achievements using high-speed atomic force microscopy (HS-AFM). The HS-AFM is now the only technique to assess structure and dynamics of single molecules, revealing molecular motor action and diffusion dynamics. From this imaging data, watching molecules at work, novel and direct insights could be gained concerning the structure, dynamics and function relationship at the single bio-molecule level. Copyright © 2011 Elsevier Ltd. All rights reserved.

  4. Inter-annual variability of sea surface temperature, wind speed and sea surface height anomaly over the tropical Indian Ocean

    Digital Repository Service at National Institute of Oceanography (India)

    Muraleedharan, P.M.; Pankajakshan, T.; Sathe, P.V.

    have made an attempt to study the annual and inter-annual variability of certain prominent processes occurring over the tropical Indian Ocean. The monthly mean values of Wind Speed (FSU), Sea Surface Temperature (REYNOLDS) and Sea Surface Height Anomaly...

  5. Full-frame, high-speed 3D shape and deformation measurements using stereo-digital image correlation and a single color high-speed camera

    Science.gov (United States)

    Yu, Liping; Pan, Bing

    2017-08-01

    Full-frame, high-speed 3D shape and deformation measurement using stereo-digital image correlation (stereo-DIC) technique and a single high-speed color camera is proposed. With the aid of a skillfully designed pseudo stereo-imaging apparatus, color images of a test object surface, composed of blue and red channel images from two different optical paths, are recorded by a high-speed color CMOS camera. The recorded color images can be separated into red and blue channel sub-images using a simple but effective color crosstalk correction method. These separated blue and red channel sub-images are processed by regular stereo-DIC method to retrieve full-field 3D shape and deformation on the test object surface. Compared with existing two-camera high-speed stereo-DIC or four-mirror-adapter-assisted singe-camera high-speed stereo-DIC, the proposed single-camera high-speed stereo-DIC technique offers prominent advantages of full-frame measurements using a single high-speed camera but without sacrificing its spatial resolution. Two real experiments, including shape measurement of a curved surface and vibration measurement of a Chinese double-side drum, demonstrated the effectiveness and accuracy of the proposed technique.

  6. Integration of Variable Speed Pumped Hydro Storage in Automatic Generation Control Systems

    Science.gov (United States)

    Fulgêncio, N.; Moreira, C.; Silva, B.

    2017-04-01

    Pumped storage power (PSP) plants are expected to be an important player in modern electrical power systems when dealing with increasing shares of new renewable energies (NRE) such as solar or wind power. The massive penetration of NRE and consequent replacement of conventional synchronous units will significantly affect the controllability of the system. In order to evaluate the capability of variable speed PSP plants participation in the frequency restoration reserve (FRR) provision, taking into account the expected performance in terms of improved ramp response capability, a comparison with conventional hydro units is presented. In order to address this issue, a three area test network was considered, as well as the corresponding automatic generation control (AGC) systems, being responsible for re-dispatching the generation units to re-establish power interchange between areas as well as the system nominal frequency. The main issue under analysis in this paper is related to the benefits of the fast response of variable speed PSP with respect to its capability of providing fast power balancing in a control area.

  7. Control and Health Monitoring of Variable Speed Wind Power Generation Systems; Period of Performance: 10 July 1997 - 10 July 2000

    Energy Technology Data Exchange (ETDEWEB)

    Song, Y. D.; Bikdash, M.; Schulz, M. J.

    2001-09-01

    This document reports accomplishments on variable speed control, furling analysis, and health monitoring of wind turbines. There are three parts, prepared by Song, Bikdash, and Schulz, respectively. The first part discusses variable-speed control of wind turbines, exploring a memory-based method for wind speed prediction and wind turbine control. The second part addresses the yaw dynamics of wind turbines, including modeling, analysis, and control. The third part of the report discusses new analytical techniques that were developed and tested to detect initial damage to prevent failures of wind turbine rotor blades.

  8. LABORATORY INVESTIGATION OF VARIABLE SPEED CONTROL OF SYNCHRONOUS GENERATOR WITH A BOOST CONVERTER FOR WIND TURBINE APPLICATIONS

    DEFF Research Database (Denmark)

    Sharma, Ranjan; Rasmussen, Tonny Wederberg

    2008-01-01

    This paper includes the experimental and simulated results of variable speed control of a synchronous generator. To achieve controlled variable speed operation, the synchronous generator is loaded with a three phase rectifier and a boost converter. The terminal voltage of the generator can...... be controlled from the converter’s duty cycle if output voltage of the converter is kept constant. This constant voltage is achieved with the help of a grid side inverter. As the speed and the terminal voltage of the generator are directly related to each other, its speed can be controlled for a given torque....... An experimental setup of a 7.5kVA generator is prepared to verify system response. Some interesting aspects concerning distributed capacitance of the generator winding and non-linear speed vs duty-cycle response are observed. Cause and effect of such problems are discussed in this paper. Efficiency measurement...

  9. Face liveness detection from a single image via diffusion speed model.

    Science.gov (United States)

    Wonjun Kim; Sungjoo Suh; Jae-Joon Han

    2015-08-01

    Spoofing using photographs or videos is one of the most common methods of attacking face recognition and verification systems. In this paper, we propose a real-time and nonintrusive method based on the diffusion speed of a single image to address this problem. In particular, inspired by the observation that the difference in surface properties between a live face and a fake one is efficiently revealed in the diffusion speed, we exploit antispoofing features by utilizing the total variation flow scheme. More specifically, we propose defining the local patterns of the diffusion speed, the so-called local speed patterns, as our features, which are input into the linear SVM classifier to determine whether the given face is fake or not. One important advantage of the proposed method is that, in contrast to previous approaches, it accurately identifies diverse malicious attacks regardless of the medium of the image, e.g., paper or screen. Moreover, the proposed method does not require any specific user action. Experimental results on various data sets show that the proposed method is effective for face liveness detection as compared with previous approaches proposed in studies in the literature.

  10. Generating single photons at gigahertz modulation-speed using electrically controlled quantum dot microlenses

    Energy Technology Data Exchange (ETDEWEB)

    Schlehahn, A.; Schmidt, R.; Hopfmann, C.; Schulze, J.-H.; Strittmatter, A.; Heindel, T., E-mail: tobias.heindel@tu-berlin.de; Reitzenstein, S. [Institut für Festkörperphysik, Technische Universität Berlin, 10623 Berlin (Germany); Gantz, L.; Schmidgall, E. R.; Gershoni, D. [The Physics Department and the Solid State Institute, Technion-Israel Institute of Technology, 32000 Haifa (Israel)

    2016-01-11

    We report on the generation of single-photon pulse trains at a repetition rate of up to 1 GHz. We achieve this speed by modulating the external voltage applied on an electrically contacted quantum dot microlens, which is optically excited by a continuous-wave laser. By modulating the photoluminescence of the quantum dot microlens using a square-wave voltage, single-photon emission is triggered with a response time as short as (281 ± 19) ps, being 6 times faster than the radiative lifetime of (1.75 ± 0.02) ns. This large reduction in the characteristic emission time is enabled by a rapid capacitive gating of emission from the quantum dot, which is placed in the intrinsic region of a p-i-n-junction biased below the onset of electroluminescence. Here, since our circuit acts as a rectifying differentiator, the rising edge of the applied voltage pulses triggers the emission of single photons from the optically excited quantum dot. The non-classical nature of the photon pulse train generated at GHz-speed is proven by intensity autocorrelation measurements with g{sup (2)}(0) = 0.3 ± 0.1. Our results combine optical excitation with fast electrical gating and thus show promise for the generation of indistinguishable single photons at rates exceeding the limitations set by the intrinsic radiative lifetime.

  11. Generating single photons at gigahertz modulation-speed using electrically controlled quantum dot microlenses

    Science.gov (United States)

    Schlehahn, A.; Schmidt, R.; Hopfmann, C.; Schulze, J.-H.; Strittmatter, A.; Heindel, T.; Gantz, L.; Schmidgall, E. R.; Gershoni, D.; Reitzenstein, S.

    2016-01-01

    We report on the generation of single-photon pulse trains at a repetition rate of up to 1 GHz. We achieve this speed by modulating the external voltage applied on an electrically contacted quantum dot microlens, which is optically excited by a continuous-wave laser. By modulating the photoluminescence of the quantum dot microlens using a square-wave voltage, single-photon emission is triggered with a response time as short as (281 ± 19) ps, being 6 times faster than the radiative lifetime of (1.75 ± 0.02) ns. This large reduction in the characteristic emission time is enabled by a rapid capacitive gating of emission from the quantum dot, which is placed in the intrinsic region of a p-i-n-junction biased below the onset of electroluminescence. Here, since our circuit acts as a rectifying differentiator, the rising edge of the applied voltage pulses triggers the emission of single photons from the optically excited quantum dot. The non-classical nature of the photon pulse train generated at GHz-speed is proven by intensity autocorrelation measurements with g(2)(0) = 0.3 ± 0.1. Our results combine optical excitation with fast electrical gating and thus show promise for the generation of indistinguishable single photons at rates exceeding the limitations set by the intrinsic radiative lifetime.

  12. Reliability of the Single-Visit Field Test of Critical Speed in Trained and Untrained Adolescents

    Directory of Open Access Journals (Sweden)

    Alfred Nimmerichter

    2015-12-01

    Full Text Available Recent studies in adults have shown that the critical intensity during running and cycling estimated from three prediction trials interspersed by 30 min is valid and reliable. To establish the reliability of the single-visit field test to determine critical speed (CS and the distance above critical speed (D′ in adolescents, 29 trained and 14 untrained participants (mean ± SD age: 17.5 ± 0.5 years performed three tests on a 400-m outdoor track separated by 48 h. Each test consisted of three distances selected to result in finishing times between 2 and 15 min that must be completed as fast as possible. CS and D′ were modeled using the linear 1/time model (Speed = D′(1/t + CS. While the coefficient of variation (CV of CS was between 2.4% and 4.3%, the CV of D′ was 9.3% to 13.6%. Also the intraclass correlation coefficient ranged from 0.919 to 0.983 for CS and from 0.325 to 0.828 for D′. The results show that the single-visit field test provides reliable estimates of CS but not D′ in trained and untrained adolescents.

  13. Torque control of synchronous and induction generators for variable speed operation of wind turbines

    Energy Technology Data Exchange (ETDEWEB)

    Carlson, Ola; Ulen, E. [Chalmers Univ. of Technology, Goeteborg (Sweden). Dept. of Electric Power Engineering

    1996-12-01

    The aim of this paper is to investigate variable speed electrical systems. Synchronous generators with diode rectifiers and line-commutated thyristor converters are compared with induction generators with force commutated transistor converters and scalar control. The system characteristics are examined regarding possible speed of response (bandwidth) of the torque control, including the sensitivity to disturbances for the drive train and also the possibility to get damping of the drive train resonance. Analyses, simulations and laboratory tests with a 40 kW machine set-up have been performed. The investigation shows that the system with synchronous generator is well suited for wind power applications. A rapid standard DC-current regulator is included in the torque control and can be used for damping of the resonance. The torque control has a bandwidth up to about 3 Hz and the DC-voltage controller up to about 1 Hz. The system with induction generator with scalar control (no transformations) is more difficult to control. A linear approach is only possible up to about 1.5 Hz. In this region it turns out that the behaviour can be visualized as an added inertia on the generator side that can be rather big. 4 refs, 9 figs

  14. Variable-Speed Induction Motor Drives for Aircraft Environmental Control Compressors

    Science.gov (United States)

    Mildice, J. W.; Hansen, I. G.; Schreiner, K. E.; Roth, M. E.

    1996-01-01

    New, more-efficient designs for aircraft jet engines are not capable of supplying the large quantities of bleed air necessary to provide pressurization and air conditioning for the environmental control systems (ECS) of the next generation of large passenger aircraft. System analysis and engineering have determined that electrically-driven ECS can help to maintain the improved fuel efficiencies; and electronic controllers and induction motors are now being developed in a NASA/NPD SBIR Program to drive both types of ECS compressors. Previous variable-speed induction motor/controller system developments and publications have primarily focused on field-oriented control, with large transient reserve power, for maximum acceleration and optimum response in actuator and robotics systems. The application area addressed herein is characterized by slowly-changing inputs and outputs, small reserve power capability for acceleration, and optimization for maximum efficiency. This paper therefore focuses on the differences between this case and the optimum response case, and shows the development of this new motor/controller approach. It starts with the creation of a new set of controller requirements. In response to those requirements, new control algorithms are being developed and implemented in an embedded computer, which is integrated into the motor controller closed loop. Buffered logic outputs are used to drive the power switches in a resonant-technology, power processor/motor-controller, at switching/resonant frequencies high enough to support efficient high-frequency induction motor operation at speeds up to 50,000-RPA

  15. Speed-variable Switched Differential Pump System for Direct Operation of Hydraulic Cylinders

    DEFF Research Database (Denmark)

    Schmidt, Lasse; Roemer, Daniel Beck; Pedersen, Henrik Clemmensen

    2015-01-01

    Efforts to overcome the inherent loss of energy due to throttling in valve driven hydraulic systems are many, and various approaches have been proposed by research communities as well as the industry. Recently, a so-called speed-variable differential pump was proposed for direct drive of hydraulic...... may seriously influence the dynamics and hence the performance during operation. This paper presents an analysis of these properties, and a redesign of the hydraulic system concept is proposed. Here the area- and displacement ratios are deliberately mismatched, causing inherent pressure build...... features are verified by comparison with the original pump concept and a conventional valve concept. Furthermore, significant improvement in energy efficiency is demonstrated under certain load conditions....

  16. Analysis of the short-term overproduction capability of variable speed wind turbines

    DEFF Research Database (Denmark)

    Hansen, Anca Daniela; Altin, Müfit; Margaris, Ioannis D.

    2014-01-01

    Emphasis in this article is on variable speed wind turbines (VSWTs) capability to provide short-term overproduction and better understanding of VSWTs’ mechanical and electrical limits to deliver such support. VSWTs’ short-term overproduction capability is of primary concern for the transmission...... system operators (TSOs) in the process of restoring critical situations during large frequency excursions in power systems with high wind power penetration. This study is conducted on a simplified generic model for VSWTs with full scale power converter (Type IV), which includes several adjustments...... and extensions of the Type IV standard wind turbine model proposed by the IEC Committee in IEC 61400-27-1. This modified standard model is able to account for dynamic features relevant for integrating active power ancillary services in wind power plants, such as frequency support capabilities. The performance...

  17. Modular Multilevel Converters Based Variable Speed Wind Turbines for Grid Faults

    DEFF Research Database (Denmark)

    Deng, Fujin; Liu, Dong; Wang, Yanbo

    2016-01-01

    The modular multilevel converter (MMC) becomes attractive in the medium- and high-power application with high modularity. In this paper, the MMC is proposed to be applied in the variable speed wind turbine (VSWT) based on the full-scale back-to-back (BTB) power converter, where the generator......-side converter is the three-level neutral-point-clamped (NPC) converter and the grid-side converter is the three-phase MMC. A control strategy is also presented for the MMC based VSWT for the second-order harmonic current elimination. The proposed MMC based VSWT and control not only mitigate the voltage ripple...... of the proposed MMC based VSWT and control are conducted with a detailed PSCAD/EMTDC model, and the results show their effectiveness....

  18. Investigation of variable spindle speed in slow tool servo-based turning of noncircular optical components

    Science.gov (United States)

    Huang, Weihai; Yu, Deping; Chen, Dongsheng; Zhang, Min; Liu, Jinguang; Yao, Jin

    2016-10-01

    Ultra-precision noncircular optical components, e.g. hyperbolic quadrupole in mass spectrometer, can be machined by diamond turning assisted by slow tool servo (STS). However, the bandwidth of STS is usually small, which limits the STS's capability in following the required tool path, leading to a large form error. To reduce the form error, this paper proposes an approach to apply variable spindle speed (VSS) to STS-based turning. Design of the VSS trajectory based on the noncircular profile of the optical component was investigated in detail. To validate the proposed approach, simulation on the application of VSS in the STS-based turning process was established and applied to the machining of typical noncircular optical components. Simulation results show that the proposed approach is effective in reducing the requirement on the bandwidth of the STS, resulting in higher form accuracy of the machined noncircular optical components.

  19. Mitigation of Wind Power Fluctuation by Active Current Control of Variable Speed Wind Turbines

    DEFF Research Database (Denmark)

    Zhang, Yunqian; Chen, Zhe; Hu, Weihao

    2013-01-01

    Wind shear and tower shadow are the sources of power fluctuation of grid connected wind turbines during continuous operation. This paper presents a simulation model of a MW-level doubly fed induction generator (DFIG) based variable speed wind turbine with a partial-scale back-to-back power...... converter in Simulink. A simple and effective method of wind power fluctuations mitigation by active current control of DFIG is proposed. It smoothes the generator output active power oscillations by adjusting the active current of the DFIG, such that the power oscillation is stored as the kinetic energy...... of the wind turbine. The simulations are performed on the NREL 1.5MW upwind reference wind turbine model. The simulation results are presented and discussed to demonstrate the validity of the proposed control method....

  20. Variable Speed Drive Characterization: Review of Measurement Techniques and Future Trends

    Directory of Open Access Journals (Sweden)

    Edoardo Fiorucci

    2013-01-01

    Full Text Available The significant advances in power electronics have permitted the implementation of sophisticated methods for control of electric motors. Each innovative electrical apparatus for industrial and automotive application must be correctly and exhaustively tested, both during the developing process and finally for the compliance test. The development of a new electrical system should be associated with a parallel design of an ad hoc measurement system, whose performance should be defined according to the features of the system under test. In recent years, the increasing interest for sensorless electric motor drives involved the development and implementation of a wide set of control techniques. This paper reviews the state and the trends of measurement techniques and instruments applied for the experimental characterization of variable speed drives.

  1. Residential Cold Climate Heat Pump (CCHP) w/Variable Speed Technology

    Energy Technology Data Exchange (ETDEWEB)

    Messmer, Craig S. [Unico, Inc., Arnold, MO (United States)

    2016-09-30

    This report summarizes the results of a three year program awarded to Unico, Inc. to commercialize a residential cold climate heat pump. Several designs were investigated. Compressors were selected using analysis from Oakridge National Laboratories followed by prototype construction and lab testing in a specially built environmental chamber capable of reaching -30°F. The initial design utilized two variable speed compressors in series with very good capacity results and acceptable efficiency at very cold temperatures. The design was then modified to reduce cost and complexity by redesigning the system using three dual-stage compressors: two in parallel followed by one in series. Extensive testing found significant challenge with oil management, reliability, weight and cost which prevented the system from being fully commercialized. Further analysis of other conceptual designs indicated that these challenges could be overcome in the future.

  2. Temporal and spatial multiplexed infrared single-photon counter based on high-speed avalanche photodiode.

    Science.gov (United States)

    Chen, Xiuliang; Ding, Chengjie; Pan, Haifeng; Huang, Kun; Laurat, Julien; Wu, Guang; Wu, E

    2017-03-15

    We report on a high-speed temporal and spatial multiplexed single-photon counter with photon-number-resolving capability up to four photons. The infrared detector combines a fiber loop to split, delay and recombine optical pulses and a 200 MHz dual-channel single-photon detector based on InGaAs/InP avalanche photodiode. To fully characterize the photon-number-resolving capability, we perform quantum detector tomography and then reconstruct its positive-operator-valued measure and the associated Wigner functions. The result shows that, despite of the afterpulsing noise and limited system detection efficiency, this temporal and spatial multiplexed single-photon counter can already find applications for large repetition rate quantum information schemes.

  3. Variable Speed Heat Pump Sizing Guide for Mixed-Humid Climates

    Energy Technology Data Exchange (ETDEWEB)

    Munk, Jeffrey D [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Odukomaiya, Adewale [Georgia Inst. of Technology, Atlanta, GA (United States); Jackson, Roderick K [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Boudreaux, Philip R [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

    2015-03-01

    The similarities and differences between different capacity units in a model family will vary depending on the manufacturer. These unit specific details are critical to choosing the VSHP capacity that will yield the highest energy savings without compromising comfort. In addition, the house construction, climate, and occupant behavior will influence the balance of the heating and cooling load on the house as well as the sensible and latent cooling demand. All of these factors need to be considered when trying to select the proper unit for the highest energy savings. Based on the simulations performed in this study, it is likely that VSHPs with an enhanced dehumidification mode can be sized up to two times the cooling load of the house without any reduction in comfort when compared to a single speed heat pump assuming a typical home and occupant behavior in the mixed-humid and cold climates.

  4. Computational Assessment of the Aerodynamic Performance of a Variable-Speed Power Turbine for Large Civil Tilt-Rotor Application

    Science.gov (United States)

    Welch, Gerard E.

    2011-01-01

    The main rotors of the NASA Large Civil Tilt-Rotor notional vehicle operate over a wide speed-range, from 100% at take-off to 54% at cruise. The variable-speed power turbine offers one approach by which to effect this speed variation. Key aero-challenges include high work factors at cruise and wide (40 to 60 deg.) incidence variations in blade and vane rows over the speed range. The turbine design approach must optimize cruise efficiency and minimize off-design penalties at take-off. The accuracy of the off-design incidence loss model is therefore critical to the turbine design. In this effort, 3-D computational analyses are used to assess the variation of turbine efficiency with speed change. The conceptual design of a 4-stage variable-speed power turbine for the Large Civil Tilt-Rotor application is first established at the meanline level. The design of 2-D airfoil sections and resulting 3-D blade and vane rows is documented. Three-dimensional Reynolds Averaged Navier-Stokes computations are used to assess the design and off-design performance of an embedded 1.5-stage portion-Rotor 1, Stator 2, and Rotor 2-of the turbine. The 3-D computational results yield the same efficiency versus speed trends predicted by meanline analyses, supporting the design choice to execute the turbine design at the cruise operating speed.

  5. High-speed bridge circuit for InGaAs avalanche photodiode single-photon detector

    Science.gov (United States)

    Hashimoto, Hirofumi; Tomita, Akihisa; Okamoto, Atsushi

    2014-02-01

    Because of low power consumption and small footprint, avalanche photodiodes (APD) have been commonly applied to photon detection. Recently, high speed quantum communication has been demonstrated for high bit-rate quantum key distribution. For the high speed quantum communication, photon detectors should operate at GHz-clock frequencies. We propose balanced detection circuits for GHz-clock operation of InGaAs-APD photon detectors. The balanced single photon detector operates with sinusoidal wave gating. The sinusoidal wave appearing in the output is removed by the subtraction from APD signal without sharp band-elimination filters. Omission of the sharp filters removes the constraint on the operating frequency of the single photon detector. We present two designs, one works with two identical APDs, the other with one APD and a low-pass filter. The sinusoidal gating enables to eliminate the gating noise even with the simple configuration of the latter design. We demonstrated the balanced single photon detector operating with 1.020GHz clock at 233 K, 193 K, and 186.5 K. The dark count probability was 4.0 x 10-4 counts/pulse with the quantum efficiency of 10% at 233K, and 1.6 x 10-4 counts/pulse at 186.5 K. These results were obtained with easily available APDs (NR8300FP-C.C, RENESASS) originally developed for optical time-domain reflectmeters.

  6. Transient and steady state performance analysis of power flow control in a DFIG variable speed wind turbine

    Science.gov (United States)

    Nwosu, Cajethan M.; Oti, Stephen E.; Ogbuka, Cosmas U.

    2017-01-01

    This paper presents transient and steady state performance analysis of power flow control in a 5.0 kW Doubly-Fed Induction Generator (DFIG) Variable Speed Wind Turbine (VSWT) under sub synchronous speed, super synchronous speed and synchronous speed modes of operation. Stator flux orientation is used for the control of the rotor-side converter (RSC) and DFIG whereas the grid (or stator) voltage orientation is the preferred choice for the control of the grid-side converter (GSC). In each of the three speeds modes, power is always supplied to the grid through the stator of the DFIG. The magnitude of net power (stator power plus rotor power) is less than stator power during the sub synchronous speed mode; it is greater than stator power during the super synchronous speed mode while it is equal to the stator power during the synchronous speed mode. In synchronous speed mode, the rotor power is zero indicating that power is neither supplied to the grid from the rotor nor supplied to the rotor from the grid; here the magnitude of net power is equal to stator power. The simulation results thus obtained in a MATLAB/SIMULINK environment laid credence to the controllability of power flow reversal in a DFIG-VSWT through back-to-back power electronic converter.

  7. Development of a high-speed single-photon pixellated detector for visible wavelengths

    Energy Technology Data Exchange (ETDEWEB)

    Raighne, Aaron Mac [Department of Physics and Astronomy, University of Glasgow, England (United Kingdom)], E-mail: aaron.macraighne@gmail.com; Teixeira, Antonio; Mathot, Serge [CERN, Geneve (Switzerland); McPhate, Jason; Vallerga, John [University of California, Berkeley (United States); Jarron, Pierre [CERN, Geneve (Switzerland); Brownlee, Colin [Marine Biological Association of the United Kingdom, Plymouth (United Kingdom); O' Shea, Val [Department of Physics and Astronomy, University of Glasgow, England (United Kingdom)

    2009-08-01

    We present the development of a high-speed, single-photon counting, Hybrid Photo Detector (HPD). The HPD consists of a vacuum tube, containing the detector assembly, sealed with a transparent optical input window. Photons incident on the photocathode eject a photoelectron into a large electric field, which accelerates the incident electron onto a silicon detector. The silicon detector is bump bonded to a Medipix readout chip. This set-up allows for the detection and readout of low incident photon intensities at rates that are otherwise unattainable with current camera technology. Reported is the fabrication of the camera that brings together a range of sophisticated design and fabrication techniques and the expected theoretical imaging performance. Applications to cellular and molecular microscopy are also described in which single-photon-counting abilities at high frame rates are crucial.

  8. Development of a high-speed single-photon pixellated detector for visible wavelengths

    CERN Document Server

    Mac Raighne, Aaron; Mathot, Serge; McPhate, Jason; Vallerga, John; Jarron, Pierre; Brownlee, Colin; O’Shea, Val

    2009-01-01

    We present the development of a high-speed, single-photon counting, Hybrid Photo Detector (HPD). The HPD consists of a vacuum tube, containing the detector assembly, sealed with a transparent optical input window. Photons incident on the photocathode eject a photoelectron into a large electric field, which accelerates the incident electron onto a silicon detector. The silicon detector is bump bonded to a Medipix readout chip. This set-up allows for the detection and readout of low incident photon intensities at rates that are otherwise unattainable with current camera technology. Reported is the fabrication of the camera that brings together a range of sophisticated design and fabrication techniques and the expected theoretical imaging performance. Applications to cellular and molecular microscopy are also described in which single-photon-counting abilities at high frame rates are crucial

  9. Assessment of power step performances of variable speed pump-turbine unit by means of hydro-electrical system simulation

    Science.gov (United States)

    Béguin, A.; Nicolet, C.; Hell, J.; Moreira, C.

    2017-04-01

    The paper explores the improvement in ancillary services that variable speed technologies can provide for the case of an existing pumped storage power plant of 2x210 MVA which conversion from fixed speed to variable speed is investigated with a focus on the power step performances of the units. First two motor-generator variable speed technologies are introduced, namely the Doubly Fed Induction Machine (DFIM) and the Full Scale Frequency Converter (FSFC). Then a detailed numerical simulation model of the investigated power plant used to simulate power steps response and comprising the waterways, the pump-turbine unit, the motor-generator, the grid connection and the control systems is presented. Hydroelectric system time domain simulations are performed in order to determine the shortest response time achievable, taking into account the constraints from the maximum penstock pressure and from the rotational speed limits. It is shown that the maximum instantaneous power step response up and down depends on the hydro-mechanical characteristics of the pump-turbine unit and of the motor-generator speed limits. As a results, for the investigated test case, the FSFC solution offer the best power step response performances.

  10. Unexpected recovery after robotic locomotor training at physiologic stepping speed: a single-case design.

    Science.gov (United States)

    Spiess, Martina R; Jaramillo, Jeffrey P; Behrman, Andrea L; Teraoka, Jeffrey K; Patten, Carolynn

    2012-08-01

    To investigate the effect of walking speed on the emergence of locomotor electromyogram (EMG) patterns in an individual with chronic incomplete spinal cord injury (SCI), and to determine whether central pattern generator activity during robotic locomotor training (RLT) transfers to volitional EMG activity during overground walking. Single-case (B-A-B; experimental treatment-withdrawal-experimental treatment) design. Freestanding rehabilitation research center. A 50-year-old man who was nonambulatory for 16 months after incomplete SCI (sub-T11). The participant completed two 6-week blocks of RLT, training 4 times per week for 30 minutes per session at walking speeds up to 5km/h (1.4m/s) over continuous bouts lasting up to 17 minutes. Surface EMG was recorded weekly during RLT and overground walking. The Walking Index for Spinal Cord Injury (WISCI-II) was assessed daily during training blocks. During week 4, reciprocal, patterned EMG emerged during RLT. EMG amplitude modulation revealed a curvilinear relationship over the range of walking speeds from 1.5 to 5km/h (1.4m/s). Functionally, the participant improved from being nonambulatory (WISCI-II 1/20), to walking overground with reciprocal stepping using knee-ankle-foot orthoses and a walker (WISCI-II 9/20). EMG was also observed during overground walking. These functional gains were maintained greater than 4 years after locomotor training (LT). Here we report an unexpected course of locomotor recovery in an individual with chronic incomplete SCI. Through RLT at physiologic walking speeds, it was possible to activate the central pattern generator even 16 months postinjury. Further, to a certain degree, improvements from RLT transferred to overground walking. Our results suggest that LT-induced changes affect the central pattern generator and allow supraspinal inputs to engage residual spinal pathways. Copyright © 2012 American Congress of Rehabilitation Medicine. Published by Elsevier Inc. All rights reserved.

  11. Investigation of a lattice Boltzmann model with a variable speed of sound

    OpenAIRE

    Buick, J.M.; Cosgrove, J. A.

    2007-01-01

    A lattice Boltzmann model is considered in which the speed of sound can be varied independently of the other parameters. The range over which the speed of sound can be varied is investigated and good agreement is found between simulations and theory. The onset of nonlinear effects due to variations in the speed of sound is also investigated and good agreement is again found with theory. It is also shown that the fluid viscosity is not altered by changing the speed of sound.

  12. On the modelling and partial-load control of variable-speed wind turbines

    Energy Technology Data Exchange (ETDEWEB)

    Novak, P. [Chalmers Univ. of Technology, Goeteborg (Sweden). School of Electrical and Computer Engineering

    1995-12-31

    The focus of this thesis is on modelling and variable-speed control of wind turbines. A physical model structure including the fundamental drive-train mode is derived and validated by system-identification experiments on a full-scale wind turbine. The resulting, parametrized model has been used as a basis for an evaluation of controllers for partial-load operation, validated by non-linear simulations. This evaluation, including several controller concepts, verifies that a sophisticated controller becomes necessary, when stretching the limits in power-loss minimization. This control strategy also demands the sampling frequency to be pushed to a high level. As a consequence, the angular-position measurements become time correlated and, in the limit, periodic. It is shown in the thesis how the resulting, operating-point-dependent effects on the measurement errors influence the estimation quality, using a stationary Kalman filter as an example. A gain-scheduling estimation approach is shown to improve the performance. 39 refs, 63 figs, 2 tabs

  13. Requirements for Large Eddy Simulation Computations of Variable-Speed Power Turbine Flows

    Science.gov (United States)

    Ameri, Ali A.

    2016-01-01

    Variable-speed power turbines (VSPTs) operate at low Reynolds numbers and with a wide range of incidence angles. Transition, separation, and the relevant physics leading to them are important to VSPT flow. Higher fidelity tools such as large eddy simulation (LES) may be needed to resolve the flow features necessary for accurate predictive capability and design of such turbines. A survey conducted for this report explores the requirements for such computations. The survey is limited to the simulation of two-dimensional flow cases and endwalls are not included. It suggests that a grid resolution necessary for this type of simulation to accurately represent the physics may be of the order of Delta(x)+=45, Delta(x)+ =2 and Delta(z)+=17. Various subgrid-scale (SGS) models have been used and except for the Smagorinsky model, all seem to perform well and in some instances the simulations worked well without SGS modeling. A method of specifying the inlet conditions such as synthetic eddy modeling (SEM) is necessary to correctly represent the inlet conditions.

  14. Ocean Current Energy Conversion System in Wallacea Region Using Variable Speed Control Approach

    Directory of Open Access Journals (Sweden)

    Aditya Sukma Nugraha

    2012-03-01

    Full Text Available Ocean Current Energy Conversion System (OCECS is a promising green energy resource in this globe. The Thermohaline circulation data indicates that the Wallacea region has the potential of ocean current energy resources. This paper is aimed to propose research and development of OCECSs to be implemented in the Wallacea region. Firstly, four types of green energy conversion systems extracted from ocean are reviewed. Their advantages and disadvantages are discussed. Secondly, the potential of OCECS in the Wallacea region is described. Third, many types of turbines used for OCECS are reviewed and the turbine type for OCECS is selected to be implemented in the Wallacea region. Fourth, control strategy is proposed.From the work reported in this paper it is concluded that it is appropriate to implement OCECSs using axial flow water turbines in the Wallacea region, and that to maximize energy conversion variable speed control approach is selected together with control of mechanism to move the turbine vertically as well as to rotate the turbine in yaw direction. 

  15. Sliding mode direct power control of RSC for DFIGs driven by variable speed wind turbines

    Directory of Open Access Journals (Sweden)

    E.G. Shehata

    2015-12-01

    Full Text Available In spite of its several advantages, a classic direct power control (DPC of doubly fed induction generators (DFIGs driven by variable speed wind turbines has some drawbacks. In this paper, a simple and robust total sliding mode controller (TSMC is designed to improve the classical DPC performance without complicating the overall scheme. The TSMC is designed to regulate the DFIG stator active and reactive powers. Two integral switching functions are selected for describing the switching surfaces of the active and reactive powers. Reaching phase stability problem of the classical sliding mode controller is avoided in the proposed TSMC. Neither current control loops nor accurate values of machine parameters are required in the proposed scheme. In addition, axes transformation of the stator voltage and current are eliminated. The grid side converter is controlled based on DPC principle to regulate both DC-link voltage and total reactive power. The feasibility of the proposed DPC scheme is validated through simulation studies on a 1.5 MW wind power generation system. The performance of the proposed and conventional DPC schemes is compared under different operating conditions.

  16. GeneratorSE: A Sizing Tool for Variable-Speed Wind Turbine Generators

    Energy Technology Data Exchange (ETDEWEB)

    Sethuraman, Latha [National Renewable Energy Laboratory (NREL), Golden, CO (United States); Dykes, Katherine L [National Renewable Energy Laboratory (NREL), Golden, CO (United States)

    2017-09-22

    This report documents a set of analytical models employed by the optimization algorithms within the GeneratorSE framework. The initial values and boundary conditions employed for the generation of the various designs and initial estimates for basic design dimensions, masses, and efficiency for the four different models of generators are presented and compared with empirical data collected from previous studies and some existing commercial turbines. These models include designs applicable for variable-speed, high-torque application featuring direct-drive synchronous generators and low-torque application featuring induction generators. In all of the four models presented, the main focus of optimization is electromagnetic design with the exception of permanent-magnet and wire-wound synchronous generators, wherein the structural design is also optimized. Thermal design is accommodated in GeneratorSE as a secondary attribute by limiting the winding current densities to acceptable limits. A preliminary validation of electromagnetic design was carried out by comparing the optimized magnetic loading against those predicted by numerical simulation in FEMM4.2, a finite-element software for analyzing electromagnetic and thermal physics problems for electrical machines. For direct-drive synchronous generators, the analytical models for the structural design are validated by static structural analysis in ANSYS.

  17. Investigating temporal trends in the explanatory variables related to the severity of drivers' injuries in single-vehicle collisions

    Directory of Open Access Journals (Sweden)

    Essam Dabbour

    2017-02-01

    Full Text Available This study identifies and quantifies the effects of different explanatory variables that increase the severity of drivers' injuries related to single-vehicle collisions involving light-duty vehicles. The research is based on utilizing logistic regression to analyze records of all traffic collisions that occurred in North Carolina for the years from 2007 to 2013. The study also investigates temporal stability of the identified explanatory variables throughout the analysis period. The identified explanatory variables include those related to the roadway, vehicle, driver, and environmental conditions. The explanatory variables related to the roadway include whether the roadway is divided or undivided, and whether it is in an urban or rural area. The explanatory variables related to the vehicle include vehicle's age, travel speed, and the type of the light-duty vehicle. The explanatory variables related to the driver include driver's age, gender, influence by alcohol or illicit drugs, and the use of seatbelt. The explanatory variables related to the environmental conditions include weather, lighting, and road surface conditions. Three of the investigated explanatory variables were found to be temporally unstable with significantly varying effects on the severity of drivers' injuries. Those temporally unstable variables include the travel speed, the type of the light-duty vehicle, and the age of the driver. All other investigated variables were found to be consistently significant throughout the analysis period. The findings of this research have the potential to help decision makers develop policies and countermeasures that reduce the severity of drivers' injuries by focusing on explanatory variables that consistently exhibit significant effects on the severity of drivers' injuries. The findings of this research also provide quantitative measures that may be used to determine the feasibility of implementing those countermeasures in reducing the

  18. Correlation between sea surface temperature and wind speed in Greenland Sea and their relationships with NAO variability

    Directory of Open Access Journals (Sweden)

    Bo Qu

    2012-09-01

    Full Text Available The North Atlantic Oscillation (NAO is one of the major causes of many recent changes in the Arctic Ocean. Generally, it is related to wind speed, sea surface temperature (SST, and sea ice cover. In this study, we analyzed the distributions of and correlations between SST, wind speed, NAO, and sea ice cover from 2003 to 2009 in the Greenland Sea at 10°W to 10°E, 65°N to 80°N. SST reached its peak in July, while wind speed reached its minimum in July. Seasonal variability of SST and wind speed was different for different regions. SST and wind speed mainly had negative correlations. Detailed correlation research was focused on the 75°N to 80°N band. Regression analysis shows that in this band, the variation of SST lagged three months behind that of wind speed. Ice cover and NAO had a positive correlation, and the correlation coefficient between ice cover and NAO in the year 2007 was 0.61. SST and NAO also had a positive correlation, and SST influenced NAO one month in advance. The correlation coefficients between SST and NAO reached 0.944 for the year 2005, 0.7 for the year 2008, and 0.74 for the year 2009 after shifting SST one month later. NAO also had a positive correlation with wind speed, and it also influenced wind speed one month in advance. The correlation coefficients between NAO and wind speed reached 0.783, 0.813, and 0.818 for the years 2004, 2005, and 2008, respectively, after shifting wind speed one month earlier.

  19. The relation between executive functioning, reaction time, naming speed, and single word reading in children with typical development and language impairments.

    Science.gov (United States)

    Messer, David; Henry, Lucy A; Nash, Gilly

    2016-09-01

    Few investigations have examined the relationship between a comprehensive range of executive functioning (EF) abilities and reading. Our investigation identified components of EF that independently predicted single word reading, and determined whether their predictive role remained when additional variables were included in the regression analyses. This provided information about the EF processes that are related to reading, and the unity and diversity of EF. This study consisted of 160 children: 88 were typically developing with no language difficulties; 72 had language impairments. The assessments involved decoding, 10 measures of EF, reaction time, naming speed, non-verbal and verbal age-equivalent scores. In the first regression analysis, which only concerned the EF variables, the following verbal forms of EF had significant relationships with decoding: working memory, fluency, planning, and inhibition. Further regression analyses included additional predictor variables: reaction time, naming speed, and age-equivalent scores. These analyses indicated that most of the EF variables continued to predict decoding even when entered with competitor variables. Furthermore, after the entry of EF variables, there were no group differences in decoding (typical vs. language difficulties). We discuss the contribution of EF and other variables to reading abilities. © 2016 The British Psychological Society.

  20. State-Space Modeling and Performance Analysis of Variable-Speed Wind Turbine Based on a Model Predictive Control Approach

    Directory of Open Access Journals (Sweden)

    H. Bassi

    2017-04-01

    Full Text Available Advancements in wind energy technologies have led wind turbines from fixed speed to variable speed operation. This paper introduces an innovative version of a variable-speed wind turbine based on a model predictive control (MPC approach. The proposed approach provides maximum power point tracking (MPPT, whose main objective is to capture the maximum wind energy in spite of the variable nature of the wind’s speed. The proposed MPC approach also reduces the constraints of the two main functional parts of the wind turbine: the full load and partial load segments. The pitch angle for full load and the rotating force for the partial load have been fixed concurrently in order to balance power generation as well as to reduce the operations of the pitch angle. A mathematical analysis of the proposed system using state-space approach is introduced. The simulation results using MATLAB/SIMULINK show that the performance of the wind turbine with the MPC approach is improved compared to the traditional PID controller in both low and high wind speeds.

  1. Variability Extraction and Synthesis via Multi-Resolution Analysis using Distribution Transformer High-Speed Power Data

    Energy Technology Data Exchange (ETDEWEB)

    Chamana, Manohar [National Renewable Energy Laboratory (NREL), Golden, CO (United States); Mather, Barry A [National Renewable Energy Laboratory (NREL), Golden, CO (United States)

    2017-10-19

    A library of load variability classes is created to produce scalable synthetic data sets using historical high-speed raw data. These data are collected from distribution monitoring units connected at the secondary side of a distribution transformer. Because of the irregular patterns and large volume of historical high-speed data sets, the utilization of current load characterization and modeling techniques are challenging. Multi-resolution analysis techniques are applied to extract the necessary components and eliminate the unnecessary components from the historical high-speed raw data to create the library of classes, which are then utilized to create new synthetic load data sets. A validation is performed to ensure that the synthesized data sets contain the same variability characteristics as the training data sets. The synthesized data sets are intended to be utilized in quasi-static time-series studies for distribution system planning studies on a granular scale, such as detailed PV interconnection studies.

  2. Prediction of walking speed using single stance force or pressure measurements in healthy subjects

    NARCIS (Netherlands)

    Keijsers, N.L.W.; Stolwijk, N.M.; Renzenbrink, G.J.; Duysens, J.

    2016-01-01

    Walking speed is one of the best measures of overall walking capacity. In plantar pressure measurements, walking speed can be assessed using contact time, but it is only moderately correlated with walking speed. The center of pressure might be of more value to indicate walking speed since walking

  3. Scour around a single Marine Hydrokinetic turbine of varying tip speed ratio and Reynolds number

    Science.gov (United States)

    Volpe, M. A.; Beninati, M. L.; Krane, M.; Fontaine, A.

    2012-12-01

    Experiments are presented to explore how the sediment scour around a single Marine Hydrokinetic (MHK) turbine varies with both tip speed ratio (TSR) and flow Reynolds number. The sediment size (d50 = 790 microns) and the turbine blade diameter (0.1016 m) were held constant for all tests. Three tip speed ratios (TSR = 5, 6, and 7) and three Reynolds numbers, based on turbine blade diameter, (ReD = 31500, 35800, and 40000) were used. The MHK device is a two-bladed horizontal axis turbine and the rotating shaft is loaded using a metal brush motor. The experiments were performed in the small-scale testing platform in the hydraulic flume facility (9.8 m long, 1.2 m wide and 0.4 m deep) at Bucknell University. For each test case, bed form topology was measured after a three-hour time interval using a traversing two-dimensional bed profiler. During the experiment, scour depth measurements at the front face of the cylindrical support structure were taken to estimate a scour rate. Measurements of the bed form were taken in 1/8 diameter increments across the width of the test section. Results show that the scour hole dimensions (depth, width, length) and deposition behind the turbine increase with both TSR and Reynolds number.

  4. Influence of travel speed on spray deposition uniformity from an air-assisted variable-rate sprayer

    Science.gov (United States)

    A newly developed LiDAR-guided air-assisted variable-rate sprayer for nursery and orchard applications was tested at various travel speeds to compare its spray deposition and coverage uniformity with constant-rate applications. Spray samplers, including nylon screens and water-sensitive papers (WSP)...

  5. An improved synchronous reference frame phase-locked loop for stand-alone variable speed constant frequency power generation systems

    DEFF Research Database (Denmark)

    Liu, Yi; Xu, Wei; Ke, Longzhang

    2017-01-01

    The phase-locked loop (PLL) based on conventional synchronous reference frame, i.e. dqPLL, is usually employed in grid-connected variable speed constant frequency (VSCF) power generation systems (PGSs). However, the voltage amplitude drop of stand-alone PGSs is often greater than that of the grid...

  6. Wave propagation speeds and source term influences in single and integral porosity shallow water equations

    Directory of Open Access Journals (Sweden)

    Ilhan Özgen

    2017-10-01

    Full Text Available In urban flood modeling, so-called porosity shallow water equations (PSWEs, which conceptually account for unresolved structures, e.g., buildings, are a promising approach to addressing high CPU times associated with state-of-the-art explicit numerical methods. The PSWE can be formulated with a single porosity term, referred to as the single porosity shallow water model (SP model, which accounts for both the reduced storage in the cell and the reduced conveyance, or with two porosity terms: one accounting for the reduced storage in the cell and another accounting for the reduced conveyance. The latter form is referred to as an integral or anisotropic porosity shallow water model (AP model. The aim of this study was to analyze the differences in wave propagation speeds of the SP model and the AP model and the implications of numerical model results. First, augmented Roe-type solutions were used to assess the influence of the source terms appearing in both models. It is shown that different source terms have different influences on the stability of the models. Second, four computational test cases were presented and the numerical models were compared. It is observed in the eigenvalue-based analysis as well as in the computational test cases that the models converge if the conveyance porosity in the AP model is close to the storage porosity. If the porosity values differ significantly, the AP model yields different wave propagation speeds and numerical fluxes from those of the BP model. In this study, the ratio between the conveyance and storage porosities was determined to be the most significant parameter.

  7. Single step high-speed printing of continuous silver lines by laser-induced forward transfer

    Energy Technology Data Exchange (ETDEWEB)

    Puerto, D., E-mail: puerto@lp3.univ-mrs.fr [Aix-Marseille University, CNRS, LP3 laboratory Campus de Luminy, C.917, Marseille (France); Biver, E. [Aix-Marseille University, CNRS, LP3 laboratory Campus de Luminy, C.917, Marseille (France); Oxford Lasers Ltd., Unit 8, Moorbrook Park, Didcot, OX11 7HP (United Kingdom); Alloncle, A.-P.; Delaporte, Ph. [Aix-Marseille University, CNRS, LP3 laboratory Campus de Luminy, C.917, Marseille (France)

    2016-06-30

    Highlights: • We have performed an experimental study on laser micro-printing of silver nanoparticle inks. • We have achieved the printing of lines in a single pass at velocities of 17 m/s (1 MHz laser). • The ejection dynamics has been investigated by means of a time-resolved imaging technique. • The control of the donor film properties is of prime importance to print lines at high velocities. • Continuous conductive lines of silver inks are laser-printed on PET flexible substrates. - Abstract: The development of high-speed ink printing process by Laser-Induced Forward Transfer (LIFT) is of great interest for the printing community. To address the problems and the limitations of this process that have been previously identified, we have performed an experimental study on laser micro-printing of silver nanoparticle inks by LIFT and demonstrated for the first time the printing of continuous conductive lines in a single pass at velocities of 17 m/s using a 1 MHz repetition rate laser. We investigated the printing process by means of a time-resolved imaging technique to visualize the ejection dynamics of single and adjacent jets. The control of the donor film properties is of prime importance to achieve single step printing of continuous lines at high velocities. We use a 30 ps pulse duration laser with a wavelength of 343 nm and a repetition rate from 0.2 to 1 MHz. A galvanometric mirror head controls the distance between two consecutives jets by scanning the focused beam along an ink-coated donor substrate at different velocities. Droplets and lines of silver inks are laser-printed on glass and PET flexible substrates and we characterized their morphological quality by atomic force microscope (AFM) and optical microscope.

  8. On higher ground: how well can dynamic body acceleration determine speed in variable terrain?

    Directory of Open Access Journals (Sweden)

    Owen R Bidder

    Full Text Available INTRODUCTION: Animal travel speed is an ecologically significant parameter, with implications for the study of energetics and animal behaviour. It is also necessary for the calculation of animal paths by dead-reckoning. Dead-reckoning uses heading and speed to calculate an animal's path through its environment on a fine scale. It is often used in aquatic environments, where transmission telemetry is difficult. However, its adoption for tracking terrestrial animals is limited by our ability to measure speed accurately on a fine scale. Recently, tri-axial accelerometers have shown promise for estimating speed, but their accuracy appears affected by changes in substrate and surface gradients. The purpose of the present study was to evaluate four metrics of acceleration; Overall dynamic body acceleration (ODBA, vectorial dynamic body acceleration (VDBA, acceleration peak frequency and acceleration peak amplitude, as proxies for speed over hard, soft and inclined surfaces, using humans as a model species. RESULTS: A general linear model (GLM showed a significant difference in the relationships between the metrics and speed depending on substrate or surface gradient. When the data from all surface types were considered together, VeDBA had the highest coefficient of determination. CONCLUSIONS: All of the metrics showed some variation in their relationship with speed according to the surface type. This indicates that changes in the substrate or surface gradient during locomotion by animals would produce errors in speed estimates, and also in dead-reckoned tracks if they were calculated from speeds based entirely on a priori calibrations. However, we describe a method by which the relationship between acceleration metrics and speed can be corrected ad hoc, until tracks accord with periodic ground truthed positions, obtained via a secondary means (e.g. VHF or GPS telemetry. In this way, dead-reckoning provides a means to obtain fine scale movement data

  9. Speed Sensorless vector control of parallel-connected three-phase two-motor single-inverter drive system

    OpenAIRE

    Gunabalan, Ramachandiran; Sanjeevikumar, Padmanaban; Blaabjerg, Frede; Wheeler, Patrick; Ojo, Joseph Olorunfemi; Ertas, Ahmet H.

    2016-01-01

    This paper presents the characteristic behavior of direct vector control of two induction motors with sensorless speed feedback having the same rating parameters, paralleled combination, and supplied from a single current-controlled pulse-width-modulated voltage-source inverter drive. Natural observer design technique is known for its simple construction, which estimates the speed and rotor fluxes. Load torque is estimated by load torque adaptation and the average rotor flux was maintained co...

  10. RESEARCH REGARDING THE ACTUAL STAGE OF USING THE MICROCONTROLLERS AND THE MICROPROCESSORS IN THE VARIABLE SPEED CONSTRUCTION

    Directory of Open Access Journals (Sweden)

    Mihai USCĂTESCU

    2013-05-01

    Full Text Available The variable speed drives industry is an aggressive and rapidly growing sector. Likeany industry, in order to remain competitive its products must have a low cost and also to addressthe need to reduce the energy consumption, of the spurious emissions introduced in the energysystem. As a result of these constraining factors it was necessary the use of smart devices capableof solving complex algorithms. This paper presents a case study on the use of microprocessors andmicrocontrollers in the construction of the speed variating devices, materialized in accomplishingof an experimental stand

  11. Variable Depth Bragg Peak Method for Single Event Effects Testing

    Science.gov (United States)

    Buchner, S.; Kanyogoro, N.; Foster, C.; O'Neill, P.

    2011-01-01

    Traditionally, accelerator SEE testing is accomplished by removing the tops of packages so that the IC chips are accessible to heavy ions. However, ICs in some advanced packages cannot be de-lidded so a different approach is used that involves grinding and/or chemically etching away part of the package and the chip from the back side. The parts are then tested from the back side with ions having sufficient range to reach the sensitive volume. More recently, the entire silicon substrate in an SOI/SRAM was removed, making it possible to use low-energy ions with shorter ranges. Where removal of part of the package is not possible, facilities at Michigan State, NASA Space Radiation Laboratory, GANIL (France) and GSI (Germany) offer high-energy heavy ions with long ranges so that the ions can reach the devices' sensitive volumes without much change in the LET. Unfortunately, a run will typically involve only one ion species having a single energy and LET due to the long time it takes to tune a new energy. The Variable Depth Bragg Peak (VDBP) method is similar to the above method in that it involves the use of high-energy heavy ions that are able to pass through the packaging material and reach the device, obviating the need to remove the package. However, the method provides a broad range of LETs from a single ion by inserting degraders in the beam that modify the ion energy and, therefore, the LET. The crux of the method involves establishing a fiduciary point for degrader thickness, i.e., where the Bragg peak is located precisely at the sensitive volume in the device, for which the measured SEU cross-section and the ion LET are both also maxima and can be calculated using a Monte-Carlo program, TRIM. Once the fiduciary point has been established, calibrated high density polyethylene (HDPE) degraders are inserted into or removed from the beam to vary the ion LET at the device in a known manner. After each change of degrader thickness, the SEU cross-section is measured

  12. Mode Transition Variable Geometry for High Speed Inlets for Hypersonic Aircraft Project

    Data.gov (United States)

    National Aeronautics and Space Administration — Hypersonic propulsion research has been a focus of the NASA aeronautics program for years. Previous high-speed cruise and space access programs have examined the...

  13. Small wind turbine for variable speed with flexible blades and automatically mechanical pitching

    DEFF Research Database (Denmark)

    Rasmussen, Tonny Wederberg; Johansen, Arne

    2017-01-01

    Wind energy have for many years been a growing alternative to fossil energy. The sizes of turbines are increasing to megawatt level. Wind turbines operate in a wide range of wind speeds but are shot down for high wind speeds. Traditional concepts are relatively stiff and have a cause stress...... power production and measurements from the sites. At the end of the paper conclusions are described....

  14. Assessing gait variability in transtibial amputee fallers based on spatial-temporal gait parameters normalized for walking speed.

    Science.gov (United States)

    Hordacre, Brenton G; Barr, Christopher; Patritti, Benjamin L; Crotty, Maria

    2015-06-01

    To determine whether normalizing spatial-temporal gait data for walking speed obtained from multiple walking trials leads to differences in gait variability parameters associated with a history of falling in people with transtibial amputations. Cross-sectional study. Rehabilitation center. People with unilateral transtibial amputations (N=45; mean age ± SD, 60.5±13.7y; 35 men [78%]) were recruited. Not applicable. Participants completed 10 consecutive walking trials using an instrumented walkway system. Primary gait parameters were walking speed and step-length, step-width, step-time, and swing-time variability. A retrospective 12-month fall history was obtained from participants. Sixteen amputees (36%) were classified as fallers. Variation in gait speed across the 10 walking trials was 2.9% (range, 1.1%-12.1%). Variability parameters of normalized gait data were significantly different from variability parameters of nonnormalized data (all Pamputees with a fall history. Copyright © 2015 American Congress of Rehabilitation Medicine. Published by Elsevier Inc. All rights reserved.

  15. Use of an expert system for the choice of variable speed drives; Application des techniques d`intelligence artificielle pour le choix des systemes d`entrainement a vitesse variable

    Energy Technology Data Exchange (ETDEWEB)

    Chetate, B.; Khaldi, T.; Boudjennah, B.; Kara, C. [Institut National des Hydrocarbures et de la Chimie de Boumerdes (Algeria). Laboratoire des Economies d`Energie Electrique

    1998-06-01

    The electrical motors variable speed is a factor of energy saving. The interest of variable speed is justified by the fact that the investment is quickly redeemable. In this study, the authors present an expert system for the rational choice of variable speed drives (IES/VSD). This system allows to take into account the varieties of electrical motors, electronics converters and complex control systems. (authors) 12 refs.

  16. Control model design to limit DC-link voltage during grid fault in a dfig variable speed wind turbine

    Science.gov (United States)

    Nwosu, Cajethan M.; Ogbuka, Cosmas U.; Oti, Stephen E.

    2017-08-01

    This paper presents a control model design capable of inhibiting the phenomenal rise in the DC-link voltage during grid- fault condition in a variable speed wind turbine. Against the use of power circuit protection strategies with inherent limitations in fault ride-through capability, a control circuit algorithm capable of limiting the DC-link voltage rise which in turn bears dynamics that has direct influence on the characteristics of the rotor voltage especially during grid faults is here proposed. The model results so obtained compare favorably with the simulation results as obtained in a MATLAB/SIMULINK environment. The generated model may therefore be used to predict near accurately the nature of DC-link voltage variations during fault given some factors which include speed and speed mode of operation, the value of damping resistor relative to half the product of inner loop current control bandwidth and the filter inductance.

  17. On control strategies for power optimization and regulation of variable speed wind turbines; Sur les strategies de commande pour l'optimisation et la regulation de puissance des eoliennes a vitesse variable

    Energy Technology Data Exchange (ETDEWEB)

    Boukhezzar, B

    2006-02-15

    The research work is dealing with variable speed wind turbines modelling and control design, in order to achieve the objectives of maximizing the extracted energy from the wind, below the rated power area in the one hand and in the other hand regulating the electric power production, above the rated power area, while reducing mechanical transient loads. For this purpose, we have studied various control strategies from linear to nonlinear based. some of the controllers that we have developed, herein appear for the first time in the relevant domain, the remaining others are an adaptation of well know controllers to the adopted wind turbine models. as matter of fact, we have derived two wind turbine models as well as a wind speed estimator. Indeed, the estimator allows obtaining the effective wind speed which cannot be measured, since the wind profile around the rotor is variable in time and space. As results, it has been shown that single input control by means of pitch angle or generator control cannot succeed to simultaneously drive the electric power output regulation and the rotor speed reference tracking. So then, our idea is to combine nonlinear dynamic state feedback torque control and pitch linear based control which turns out to be the best strategy. In addition, the validation of the controllers performance, using a high turbulence wind speed profile, has been performed through wind turbine simulators provided by nrel (national renewable energy laboratory, golden, co), has confirmed the theoretical results and has led to quite satisfactory conclusions in terms of energy capture optimization, power regulation and disturbances strong rejection as well. (author)

  18. Variable-Speed Generation Subsystem Using the Doubly-Fed Generator; Period of Performance February 9, 1994 - April 30, 1999

    Energy Technology Data Exchange (ETDEWEB)

    Weigand, C.H.; Lauw, H.K.; Marckx, D.A. (Electronic Power Conditioning Incorporated)

    2000-12-18

    Over the past decade, fixed-speed, utility-scale wind turbines have technically advanced to a point where they can economically complete against nuclear and fossil-fuel-based power plants in geographical areas with a sufficient wind resource. The objective of this subcontract was to compare various electrical topologies allowing variable-speed turbine operation, identify the most suitable for a 275-kW (or larger) utility-scale wind turbine, and then design, build, lab test, and field test this variable-speed generation subsystem based on the previously identified optimum approach. Preliminary tests of the controls for a doubly fed variable-speed generation system rated at 750 kW were performed on a wind turbine. A 275-kW VSGS was thoroughly tested in the laboratory and on a wind turbine. Using field-oriented control, excellent dynamic behavior of the drive train was demonstrated, acoustic tests revealed an 11 dB reduction in turbine noise in low-wind, low-RPM operation compared to fixed-speed operation. The overall efficiency of the electrical system suffered from inadequate efficiency of the power converter at low power. Consequently, a different converter topology has been proposed that will satisfy both efficiency and power quality requirements for future use. This report provides information on all aspects of the project, including events that were unanticipated at the outset. A great deal of information is available in the references, comprised of NREL reports, journal articles, and conference papers on specific project results.

  19. Characterization of gear faults in variable rotating speed using Hilbert-Huang Transform and instantaneous dimensionless frequency normalization

    Science.gov (United States)

    Wu, T. Y.; Chen, J. C.; Wang, C. C.

    2012-07-01

    The objective of this research is to investigate the feasibility of utilizing the instantaneous dimensionless frequency (DLF) normalization and Hilbert-Huang Transform (HHT) to characterize the different gear faults in case of variable rotating speed. The normalized DLF of the vibration signals are calculated based on the rotating speed of shaft and the instantaneous frequencies of Intrinsic Mode Functions (IMFs) which are decomposed by Empirical Mode Decomposition (EMD) process. The faulty gear features on DLF-energy distribution of vibration signal can be extracted without the presence of shaft rotating speed, so that the proposed approach can be applied for characterizing the malfunctions of gearbox system under variable shaft rotating speed. A test rig of gear transmission system is performed to illustrate the gear faults, including worn tooth, broken tooth and gear unbalance. Different methods to determine the instantaneous frequency are employed to verify the consistence of characterization results. The DLF-energy distributions of vibration signals are investigated in different faulty gear conditions. The analysis results demonstrate the capability and effectiveness of the proposed approach for characterizing the gear malfunctions at the DLFs corresponding to the meshing frequency as well as the shaft rotating frequency. The support vector machine (SVM) is then employed to classify the vibration patterns of gear transmission system at different malfunctions. Using the energy distribution at the characteristic DLFs as the features, the different fault types of gear can be identified by SVM with high accuracy.

  20. Single-quadrature continuous-variable quantum key distribution

    DEFF Research Database (Denmark)

    Gehring, Tobias; Jacobsen, Christian Scheffmann; Andersen, Ulrik Lund

    2016-01-01

    Most continuous-variable quantum key distribution schemes are based on the Gaussian modulation of coherent states followed by continuous quadrature detection using homodyne detectors. In all previous schemes, the Gaussian modulation has been carried out in conjugate quadratures thus requiring two...... commercialization of continuous-variable quantum key distribution, provided that the low noise requirement can be achieved....

  1. Predicting Word Reading and Comprehension with Executive Function and Speed Measures Across Development: A Latent Variable Analysis

    Science.gov (United States)

    Christopher, Micaela E.; Miyake, Akira; Keenan, Janice M.; Pennington, Bruce; DeFries, John C.; Wadsworth, Sally J.; Willcutt, Erik; Olson, Richard K.

    2012-01-01

    The present study explored whether different executive control and speed measures (working memory, inhibition, processing speed, and naming speed) independently predict individual differences in word reading and reading comprehension. Although previous studies suggest these cognitive constructs are important for reading, we analyze the constructs simultaneously to test whether each is a unique predictor. We used latent variables from 483 participants (ages 8 to 16) to portion each cognitive and reading construct into its unique and shared variance. In these models we address two specific issues: (a) given that our wide age range may span the theoretical transition from “learning to read” to “reading to learn,” we first test whether the relation between word reading and reading comprehension is stable across two age groups (ages 8 to 10 and 11 to 16); and (b) the main theoretical question of interest: whether what is shared and what is separable for word reading and reading comprehension are associated with individual differences in working memory, inhibition, and measures of processing and naming speed. The results indicated that: (a) the relation between word reading and reading comprehension is largely invariant across the age groups; (b) working memory and general processing speed, but not inhibition or the speeded naming of non-alphanumeric stimuli, are unique predictors of both word reading and comprehension, with working memory equally important for both reading abilities and processing speed more important for word reading. These results have implications for understanding why reading comprehension and word reading are highly correlated yet separable. PMID:22352396

  2. Predicting word reading and comprehension with executive function and speed measures across development: a latent variable analysis.

    Science.gov (United States)

    Christopher, Micaela E; Miyake, Akira; Keenan, Janice M; Pennington, Bruce; DeFries, John C; Wadsworth, Sally J; Willcutt, Erik; Olson, Richard K

    2012-08-01

    The present study explored whether different executive control and speed measures (working memory, inhibition, processing speed, and naming speed) independently predict individual differences in word reading and reading comprehension. Although previous studies suggest these cognitive constructs are important for reading, the authors analyze the constructs simultaneously to test whether each is a unique predictor. Latent variables from 483 participants (ages 8-16 years) were used to portion each cognitive and reading construct into its unique and shared variance. In these models 2 specific issues are addressed: (a) Given that the wide age range may span the theoretical transition from "learning to read" to "reading to learn," the authors first test whether the relation between word reading and reading comprehension is stable across 2 age groups (ages 8-10 and 11-16); and (b) the main theoretical question of interest: whether what is shared and what is separable for word reading and reading comprehension are associated with individual differences in working memory, inhibition, and measures of processing and naming speed. The results indicated that (a) the relation between word reading and reading comprehension is largely invariant across the age groups, and (b) working memory and general processing speed, but not inhibition or the speeded naming of non-alphanumeric stimuli, are unique predictors of both word reading and comprehension, with working memory equally important for both reading abilities and processing speed more important for word reading. These results have implications for understanding why reading comprehension and word reading are highly correlated yet separable . (PsycINFO Database Record (c) 2012 APA, all rights reserved).

  3. Active Power and Flux Control of a Self-Excited Induction Generator for a Variable-Speed Wind Turbine Generation

    Energy Technology Data Exchange (ETDEWEB)

    Na, Woonki; Muljadi, Eduard; Leighty, Bill; Kim, Jonghoon

    2017-05-11

    A Self-Excited Induction Generation (SEIG) for a variable speed wind turbine generation(VS-WG) is normally considered to be a good candidate for implementation in stand-alone applications such as battery charging, hydrogenation, water pumping, water purification, water desalination, and etc. In this study, we have examined a study on active power and flux control strategies for a SEIG for a variable speed wind turbine generation. The control analysis for the proposed system is carried out by using PSCAD software. In the process, we can optimize the control design of the system, thereby enhancing and expediting the control design procedure for this application. With this study, this control design for a SEIG for VS-WG can become the industry standard for analysis and development in terms of SEIG.

  4. Single event upset studies under neutron radiation of a high speed digital optical data link

    CERN Document Server

    Andrieux, M L; Evans, G; Gallin-Martel, L; Lundqvist, J M; Pearce, M; Rethore, F; Rydström, S; Stroynowski, R; Ye, J

    2000-01-01

    The results from a series of neutron irradiation tests of a high speed digital optical data link based on a commercial serialiser, commonly known as 'G-link', and a vertical cavity surface emitting laser are described. The link was developed as a candidate for the front-end readout of the ATLAS electromagnetic calorimeter. The components at the emitting end of the link were unaffected by neutron irradiation levels exceeding those expected during 10 years of LHC running. However, the link suffered from single event upsets (SEU) when irradiated with energetic neutrons. A very general method based on the Burst Generation Rate (BGR) model has been developed and is used to extrapolate the error rate observed during tests to that expected at the LHC. A model independent extrapolation was used to check the BGR approach and the results were consistent. To reduce the SEU rate and the deadtime it implies, a Dual G-link system was built and tested with neutron radiation. 17 Refs.

  5. Robust control for variable-speed two-bladed horizontal-axis wind turbines via chattering control

    OpenAIRE

    Acho Zuppa, Leonardo; Vidal Seguí, Yolanda; Pozo Montero, Francesc

    2009-01-01

    A robust controller with chattering is proposed for variable speed control of a two-bladed horizontal axis wind turbine similar to the DOE MOD-0 model. The chattering term provides robustness against un-modeled non-linearities. To validate the performance of the proposed controller, a dynamic wind model has also been developed. This model is based on using an harmonic oscillator with an additive white noise to capture some stochastic behavior of the wind perturbation.

  6. Full-shipload tramp ship routing and scheduling with variable speeds

    DEFF Research Database (Denmark)

    Wen, M.; Røpke, Stefan; Petersen, Hanne Løhmann

    2016-01-01

    This paper investigates the simultaneous optimization problem of routing and sailing speed in the context of full-shipload tramp shipping. In this problem, a set of cargoes can be transported from their load to discharge ports by a fleet of heterogeneous ships of different speed ranges and load...... in the tanker business. To solve the problem, a three-index mixed integer linear programming formulation as well as a set packing formulation is presented. A novel Branch-and-Price algorithm with efficient data preprocessing and heuristic column generation is proposed. The computational results on the test...

  7. Gait Speed and Variability for Usual Pace and Pedestrian Crossing Conditions in Older Adults Using the GAITRite Walkway

    Directory of Open Access Journals (Sweden)

    Kristin C. Brown BSc

    2015-12-01

    Full Text Available Objectives: To determine gait characteristics of community-dwelling older adults at different speeds and during a crosswalk simulation. Methods: Twenty-two older adults completed walking trials at self-selected slow, usual, and fast paces, and at a crosswalk simulation, using the GAITRite walkway. These objective measures were complemented by self-report health and mobility questionnaires. Results: Gait speeds at self-selected slow, usual, and fast paces were 98.7 (18.1 cm/s, 140.9 (20.4 cm/s, and 174.0 (20.6 cm/s, respectively, and at simulated crosswalk conditions was 144.2 (22.3 cm/s. For usual pace, right step length variability was 2.0 (1.4 cm and step time variability was 13.6 (7.2 ms, compared with 2.4 (1.3 cm and 17.3 (9.7 ms, respectively, for crosswalk conditions. Discussion: Our sample of healthy older adults walked at a speed exceeding standards for crossing urban streets; however, in response to a crosswalk signal, participants adopted a significantly faster and more variable gait.

  8. Flicker Mitigation by Individual Pitch Control of Variable Speed Wind Turbines With DFIG

    DEFF Research Database (Denmark)

    Zhang, Yunqian; Chen, Zhe; Hu, Weihao

    2014-01-01

    generatorto investigate the flicker emission and mitigation issues. An individual pitch control (IPC) strategy is proposed to reduce the flicker emission at different wind speed conditions. The IPC scheme is proposed and the individual pitch controller is designed according to the generator active power...

  9. Design of Stirrer Impeller with Variable Operational Speed for a Food Waste Homogenizer

    Directory of Open Access Journals (Sweden)

    Idris A. Kayode

    2016-05-01

    Full Text Available A conceptualized impeller called KIA is designed for impact agitation of food waste in a homogenizer. A comparative analysis of the performance of KIA is made with three conventional impeller types, Rushton, Anchor, and Pitched Blade. Solid–liquid mixing of a moisture-rich food waste is simulated under various operational speeds, in order to compare the dispersions and thermal distributions at homogenous slurry conditions. Using SolidWorks, the design of the impellers employs an Application Programming Interface (API which acts as the canvas for creating a graphical user interface (GUI for automation of its assembly. A parametric analysis of the homogenizer, at varying operational speeds, enables the estimation of the critical speed of the mixing shaft diameter and the deflection under numerous mixing conditions and impeller configurations. The numerical simulation of the moisture-rich food waste (approximated as a Newtonian carrot–orange soup is performed with ANSYS CFX v.15.0. The velocity and temperature field distribution of the homogenizer for various impeller rotational speeds are analyzed. It is anticipated that the developed model will help in the selection of a suitable impeller for efficient mixing of food waste in the homogenizer.

  10. Variable-frequency inverter controls torque, speed, and braking in ac induction motors

    Science.gov (United States)

    Nola, F. J.

    1974-01-01

    Dc to ac inverter provides optimum frequency and voltage to ac induction motor, in response to different motor-load and speed requirements. Inverter varies slip frequency of motor in proportion to required torque. Inverter protects motor from high current surges, controls negative slip to apply braking, and returns energy stored in momentum of load to dc power source.

  11. Control design for a pitch-regulated, variable speed wind turbine

    Energy Technology Data Exchange (ETDEWEB)

    Hansen, M.H.; Hansen, A.; Larsen, T.J.; Oeye, S.; Soerensen, P.; Fuglsang, P.

    2005-01-01

    The three different controller designs presented herein are similar and all based on PI-regulation of rotor speed and power through the collective blade pitch angle and generator moment. The aeroelastic and electrical modelling used for the time-domain analysis of these controllers are however different, which makes a directly quantitative comparison difficult. But there are some observations of similar behaviours should be mentioned: 1) Very similar step responses in rotor speed, pitch angle, and power are seen for simulations with steps in wind speed. 2) All controllers show a peak in power for wind speed step-up over rated wind speed, which can be almost removed by changing the parameters of the frequency converter. 3) Responses of rotor speed, pitch angle, and power for different simulations with turbulent inflow are similar for all three controllers. Again, there seems to be an advantage of tuning the parameters of the frequency converter to obtain a more constant power output. The dynamic modelling of the power controller is an important result for the inclusion of generator dynamics in the aeroelastic modelling of wind turbines. A reduced dynamic model of the relation between generator torque and generator speed variations is presented; where the integral term of the inner PI-regulator of rotor current is removed be-cause the time constant is very small compared to the important aeroelastic frequencies. It is shown how the parameters of the transfer function for the remaining control system with the outer PI-regulator of power can be derived from the generator data sheet. The main results of the numerical optimisation of the control parameters in the pitch PI-regulator performed in Chapter 6 are the following: 1) Numerical optimization can be used to tune controller parameters, especially when the optimization is used as refinement of a qualified initial guess. 2) The design model used to calculate the initial value parameters, as described in Chapter 3

  12. Speed Sensorless vector control of parallel-connected three-phase two-motor single-inverter drive system

    DEFF Research Database (Denmark)

    Gunabalan, Ramachandiran; Sanjeevikumar, Padmanaban; Blaabjerg, Frede

    2016-01-01

    This paper presents the characteristic behavior of direct vector control of two induction motors with sensorless speed feedback having the same rating parameters, paralleled combination, and supplied from a single current-controlled pulse-width-modulated voltage-source inverter drive. Natural obs...

  13. Single-mode temperature and polarisation-stable high-speed 850nm vertical cavity surface emitting lasers

    Science.gov (United States)

    Nazaruk, D. E.; Blokhin, S. A.; Maleev, N. A.; Bobrov, M. A.; Kuzmenkov, A. G.; Vasil'ev, A. P.; Gladyshev, A. G.; Pavlov, M. M.; Blokhin, A. A.; Kulagina, M. M.; Vashanova, K. A.; Zadiranov, Yu M.; Fefelov, A. G.; Ustinov, V. M.

    2014-12-01

    A new intracavity-contacted design to realize temperature and polarization-stable high-speed single-mode 850 nm vertical cavity surface emitting lasers (VCSELs) grown by molecular-beam epitaxy is proposed. Temperature dependences of static and dynamic characteristics of the 4.5 pm oxide aperture InGaAlAs VCSEL were investigated in detail. Due to optimal gain-cavity detuning and enhanced carrier localization in the active region the threshold current remains below 0.75 mA for the temperature range within 20-90°C, while the output power exceeds 1 mW up to 90°C. Single-mode operation with side-mode suppression ratio higher than 30 dB and orthogonal polarization suppression ratio more than 18 dB was obtained in the whole current and temperature operation range. Device demonstrates serial resistance less than 250 Ohm, which is rather low for any type of single-mode short- wavelength VCSELs. VCSEL demonstrates temperature robust high-speed operation with modulation bandwidth higher than 13 GHz in the entire temperature range of 20-90°C. Despite high resonance frequency the high-speed performance of developed VCSELs was limited by the cut-off frequency of the parasitic low pass filter created by device resistances and capacitances. The proposed design is promising for single-mode high-speed VCSEL applications in a wide spectral range.

  14. Non Linear, Time Variant Speed Control of a Single Phase Hybrid Switched Reluctance Motor

    DEFF Research Database (Denmark)

    Jakobsen, Uffe; Ahn, Jin Woo

    2009-01-01

    A high torque ripple in a given motor always presents a challenge for the speed control, since this ripple may lead to excessive actuation and ultimately may even lead to instability. The conventional solution is to low pass filter the measured speed, but this lowers dynamic control performance...

  15. Individual Pitch Control for Mitigation of Power Fluctuation of Variable Speed Wind Turbines

    DEFF Research Database (Denmark)

    Zhang, Yunqian; Hu, Weihao; Chen, Zhe

    2012-01-01

    Grid connected wind turbines are the sources of power fluctuations during continuous operation due to wind speed variation, wind shear and tower shadow effects. This paper presents an individual pitch control (IPC) strategy to mitigate the wind turbine power fluctuation at both above and below...... the rated wind speed conditions. Three pitch angles are adjusted separately according to the generator output power and the azimuth angle of the wind turbine. The IPC strategy scheme is proposed and the individual pitch controller is designed. The simulations are performed on the NREL (National Renewable...... Energy Laboratory) 1.5MW upwind reference wind turbine model. The simulation results are presented and discussed to show the validity of the proposed control method....

  16. Control design for a pitch-regulated, variable speed wind turbine

    DEFF Research Database (Denmark)

    Hansen, M.H.; Hansen, Anca Daniela; Larsen, Torben J.

    2005-01-01

    The three different controller designs presented herein are similar and all based on PI-regulation of rotor speed and power through the collective blade pitch angle and generator moment. The aeroelastic and electrical modelling used for the time-domainanalysis of these controllers are however...... torque and generator speed variations is presented;where the integral term of the inner PI-regulator of rotor current is removed be-cause the time constant is very small compared to the important aeroelastic frequencies. It is shown how the parameters of the transfer function for the remaining...... controlsystem with the outer PI-regulator of power can be derived from the generator data sheet. The main results of the numerical optimisation of the control parameters in the pitch PI-regulator performed in Chapter 6 are the following: • Numericaloptimization can be used to tune controller parameters...

  17. Vibrational behavior of a soundbox in an atmosphere with a variable speed of sound.

    Science.gov (United States)

    Chen, Mo; Kotlicki, Andrzej; Waltham, Chris; Wolfe, Nathan; Yu, Jing Fei; Zhu, Chenchong

    2012-03-01

    This paper describes a semi-quantitative method, suitable for a student laboratory exercise that shows that the acoustic properties of the soundbox of a musical instrument depend on the sound speed of the atmosphere surrounding and filling the instrument. A gas tent was constructed and used to enclose instruments in helium, carbon dioxide and mixtures thereof, allowing the sound speed to be varied from 250 to 1000 m/s. Soundboard admittance data were taken using a guitar and a violin as examples. The data, expressed as contour plots, show clearly the qualitative relationship between air and wood modes, and the guitar data are compared with a simple mechanical model. Experimental details of the construction and operation of gas tent are given, with attention paid to safety issues. © 2012 Acoustical Society of America

  18. Verification and Quantification of Single Event Effects on High Speed SRAM in Terrestrial Environments

    Science.gov (United States)

    Huff, H.; You, Z.; Williams, T.; Nichols, T.; Attia, J.; Fogarty, T. N.; Kirby, K.; Wilkins, R.; Lawton, R.

    1998-01-01

    As integrated circuits become more sensitive to charged particles and neutrons, anomalous performance due to single event effects (SEE) is a concern and requires experimental verification and quantification. The Center for Applied Radiation Research (CARR) at Prairie View A&M University has developed experiments as a participant in the NASA ER-2 Flight Program, the APEX balloon flight program and the Student Launch Program. Other high altitude and ground level experiments of interest to DoD and commercial applications are being developed. The experiment characterizes the SEE behavior of high speed and high density SRAM's. The system includes a PC-104 computer unit, an optical drive for storage, a test board with the components under test, and a latchup detection and reset unit. The test program will continuously monitor the stored checkerboard data pattern in the SW and record errors. Since both the computer and the optical drive contain integrated circuits, they are also vulnerable to radiation effects. A latchup detection unit with discrete components will monitor the test program and reset the system when necessary. The first results will be obtained from the NASA ER-2 flights, which are now planned to take place in early 1998 from Dryden Research Center in California. The series of flights, at altitudes up to 70,000 feet, and a variety of flight profiles should yield a distribution of conditions for correlating SEES. SEE measurements will be performed from the time of aircraft power-up on the ground throughout the flight regime until systems power-off after landing.

  19. Offline identification of imagined speed of wrist movements in paralyzed ALS patients from single-trial EEG

    Directory of Open Access Journals (Sweden)

    Ying Gu

    2009-08-01

    Full Text Available The study investigated the possibility of identifying the speed of an imagined movement from EEG recordings in amyotrophic lateral sclerosis (ALS patients. EEG signals were acquired from four ALS patients during imagination of wrist extensions at two speeds (fast and slow, each repeated up to 100 times in random order. The movement-related cortical potentials (MRCPs and averaged sensorimotor rhythm associated with the two tasks were obtained from the EEG recordings. Moreover, offline single-trial EEG classification was performed with discrete wavelet transform for feature extraction and support vector machine for classification. The speed of the task was encoded in the time delay of peak negativity in the MRCPs, which was shorter for faster than for slower movements. The average single-trial misclassification rate between speeds was 30.4 ± 3.5 % when the best scalp location and time interval were selected for each individual. The scalp location and time interval leading to the lowest misclassification rate varied among patients. The results indicate that the imagination of movements at different speeds is a viable strategy for controlling a brain-computer interface system by ALS patients.

  20. Relationship of Speed, Agility, Neuromuscular Power, and Selected Anthropometrical Variables and Performance Results of Male and Female Junior Tennis Players.

    Science.gov (United States)

    Munivrana, Goran; Filipčić, Aleš; Filipčić, Tjaša

    2015-07-01

    The aim of the study was to analyses the relation between the selected speed, agility, and neuromuscular power test items. The sample of subjects consisted of 154 male and 152 female young tennis players. Using six motor and three anthropometrical tests we investigate differences between males and females and between two age categories. Finally, we analyzed the relation between motor and anthropometrical tests and a player's tennis performance. The correlation between the two agility test items and 5-m sprint is very large in male players, while only moderate with 20-m sprint in female category. Male tennis players have higher correlations between speed test items and neuromuscular test items. The speed test item (5-m sprint) has large correlation with a player's tennis performance. One-way analysis of variance results indicated that young male tennis players performed significantly better than females in all motor test items. Significant differences between genders have not been revealed only in the body mass index. Differences between the males aged 18& under and 16& under have been noted as significant in all test items, except the vertical jump, while differences between the females have been noted as significant in three anthropometrical tests, quarter jump, and the fan-drill test. Regression analyses have shown that the system of prediction variables explains a relatively small part of variance (46%--males and 40%--females). In both genders, it has been revealed that test items measuring speed significantly influence a player's tennis performance.

  1. Computational Study of the Impact of Unsteadiness on the Aerodynamic Performance of a Variable- Speed Power Turbine

    Science.gov (United States)

    Welch, Gerard E.

    2012-01-01

    The design-point and off-design performance of an embedded 1.5-stage portion of a variable-speed power turbine (VSPT) was assessed using Reynolds-Averaged Navier-Stokes (RANS) analyses with mixing-planes and sector-periodic, unsteady RANS analyses. The VSPT provides one means by which to effect the nearly 50 percent main-rotor speed change required for the NASA Large Civil Tilt-Rotor (LCTR) application. The change in VSPT shaft-speed during the LCTR mission results in blade-row incidence angle changes of as high as 55 . Negative incidence levels of this magnitude at takeoff operation give rise to a vortical flow structure in the pressure-side cove of a high-turn rotor that transports low-momentum flow toward the casing endwall. The intent of the effort was to assess the impact of unsteadiness of blade-row interaction on the time-mean flow and, specifically, to identify potential departure from the predicted trend of efficiency with shaft-speed change of meanline and 3-D RANS/mixing-plane analyses used for design.

  2. Advantages of variable-speed operation of hydraulic turbo-engines; Vorteile durch den drehzahlvariablen Betrieb von hydraulischen Stroemungsmaschinen

    Energy Technology Data Exchange (ETDEWEB)

    Harbort, T. [Stuttgart Univ. (Germany). Inst. fuer Stroemungsmechanik und Hydraulische Stroemungsmaschinen

    1997-12-31

    The performance of current hydraulic turbo-engines in the variable speed sector is monitored and judged. The study covers radial and axial engines as well as Pelton turbines. Variable-speed operation of hydraulic turbo-engines can be realized by means of different combinations of electrical rotating machines and frequency converters. The operating range of the frequency converter plays an important role in the optimization of performance and is taken into account. The smoothness of run of reaction turbines and their cavitation performance can be enhanced by speed regulation. But above all, efficiency is more or less substantially enhanced during partial load or in the case of greatly varying heights of drop. The latter holds true also of Pelton turbines. (orig.) [Deutsch] Das Betriebsverhalten der gaengigen hydraulischen Stroemungsmaschinen wird in Hinblick auf den drehzahlvariablen Betrieb erfasst und beurteilt. Die Untersuchung erfolgt fuer Radialmaschinen, Axialmaschinen und Peltonturbinen. Der drehzahlvariable Betrieb hydraulischer Stroemungsmaschinen kann mit verschiedenen Kombinationen von elektrischen Maschinen und Frequenzumrichtern realisiert werden. Der Arbeitsbereich des Frequenzumrichters spielt eine wichtige Rolle fuer die Optimierung des Betriebsverhaltens und wird beruecksichtigt. Bei Ueberdruckturbinen kann man durch Drehzahlregelung eine groessere Laufruhe sowie ein guenstigeres Kavitationsverhalten erreichen. Vor allem aber sind im Teillastbereich oder bei stark schwankenden Fallhoehen mehr oder weniger grosse Wirkungsgradgewinne erzielbar. Das letztere gilt auch fuer Peltonturbinen. (orig.)

  3. Examining Multiple Variables within a Single ER Setting

    Science.gov (United States)

    Karlin, Omar; Romanko, Rick

    2010-01-01

    This study examined the gains in student affect, vocabulary, and reading fluency for 110 university students in an extensive reading program in Japan. It was important to measure all of these dimensions within a single study and teaching methodology, so gains could be appropriately compared against each other. The adopted teaching methodology was…

  4. EEG Single-Trial Detection of Gait Speed Changes during Treadmill Walk.

    Directory of Open Access Journals (Sweden)

    Giuseppe Lisi

    Full Text Available In this study, we analyse the electroencephalography (EEG signal associated with gait speed changes (i.e. acceleration or deceleration. For data acquisition, healthy subjects were asked to perform volitional speed changes between 0, 1, and 2 Km/h, during treadmill walk. Simultaneously, the treadmill controller modified the speed of the belt according to the subject's linear speed. A classifier is trained to distinguish between the EEG signal associated with constant speed gait and with gait speed changes, respectively. Results indicate that the classification performance is fair to good for the majority of the subjects, with accuracies always above chance level, in both batch and pseudo-online approaches. Feature visualisation and equivalent dipole localisation suggest that the information used by the classifier is associated with increased activity in parietal areas, where mu and beta rhythms are suppressed during gait speed changes. Specifically, the parietal cortex may be involved in motor planning and visuomotor transformations throughout the online gait adaptation, which is in agreement with previous research. The findings of this study may help to shed light on the cortical involvement in human gait control, and represent a step towards a BMI for applications in post-stroke gait rehabilitation.

  5. Novel HDD-type SNDM ferroelectric data storage system aimed at high-speed data transfer with single probe operation.

    Science.gov (United States)

    Hiranaga, Yoshiomi; Uda, Tomoya; Kurihashi, Yuichi; Tanaka, Kenkou; Cho, Yasuo

    2007-12-01

    In this study, several read/write tests were conducted using a novel ferroelectric data storage test system equipped with a spindle motor, targeted at high-speed data transfer using a single probe head. A periodically inverted signal can be read out correctly with a bit rate of 100 kbps using this test system, and 10 Mbps data transfer is also possible during writing operations. The effect of a dc-offset voltage applied to the writing waveform with high-speed probe scanning is discussed. In addition, a novel noncontact probe height control technique was adopted to solve the problem of tip abrasion.

  6. Effect of trotting speed on kinematic variables measured by use of extremity-mounted inertial measurement units in nonlame horses performing controlled treadmill exercise.

    Science.gov (United States)

    Cruz, Antonio M; Vidondo, Beatriz; Ramseyer, Alessandra A; Maninchedda, Ugo E

    2018-02-01

    OBJECTIVE To assess effects of speed on kinematic variables measured by use of extremity-mounted inertial measurement units (IMUs) in nonlame horses performing controlled exercise on a treadmill. ANIMALS 10 nonlame horses. PROCEDURES 6 IMUs were attached at predetermined locations on 10 nonlame Franches Montagnes horses. Data were collected in triplicate during trotting at 3.33 and 3.88 m/s on a high-speed treadmill. Thirty-three selected kinematic variables were analyzed. Repeated-measures ANOVA was used to assess the effect of speed. RESULTS Significant differences between the 2 speeds were detected for most temporal (11/14) and spatial (12/19) variables. The observed spatial and temporal changes would translate into a gait for the higher speed characterized by increased stride length, protraction and retraction, flexion and extension, mediolateral movement of the tibia, and symmetry, but with similar temporal variables and a reduction in stride duration. However, even though the tibia coronal range of motion was significantly different between speeds, the high degree of variability raised concerns about whether these changes were clinically relevant. For some variables, the lower trotting speed apparently was associated with more variability than was the higher trotting speed. CONCLUSIONS AND CLINICAL RELEVANCE At a higher trotting speed, horses moved in the same manner (eg, the temporal events investigated occurred at the same relative time within the stride). However, from a spatial perspective, horses moved with greater action of the segments evaluated. The detected changes in kinematic variables indicated that trotting speed should be controlled or kept constant during gait evaluation.

  7. Encoderless Model Predictive Control of Doubly-Fed Induction Generators in Variable-Speed Wind Turbine Systems

    Science.gov (United States)

    Abdelrahem, Mohamed; Hackl, Christoph; Kennel, Ralph

    2016-09-01

    In this paper, an encoderless finite-control-set model predictive control (FCS-MPC) strategy for doubly-fed induction generators (DFIGs) based on variable-speed wind turbine systems (WTSs) is proposed. According to the FCS-MPC concept, the discrete states of the power converter are taken into account and the future converter performance is predicted for each sampling period. Subsequently, the voltage vector that minimizes a predefined cost function is selected to be applied in the next sampling instant. Furthermore, a model reference adaptive system (MRAS) observer is used to estimate the rotor speed and position of the DFIG. Estimation and control performance of the proposed encoderless control method are validated by simulation results for all operation conditions. Moreover, the performance of the MRAS observer is tested under variations of the DFIG parameters.

  8. Single mode variable-sensitivity fiber optic sensors

    Science.gov (United States)

    Murphy, K. A.; Fogg, B. R.; Gunther, M. F.; Claus, R. O.

    1992-01-01

    We review spatially-weighted optical fiber sensors that filter specific vibration modes from one dimensional beams placed in clamped-free and clamped-clamped configurations. The sensitivity of the sensor is varied along the length of the fiber by tapering circular-core, dual-mode optical fibers. Selective vibration mode suppression on the order of 10 dB was obtained. We describe experimental results and propose future extensions to single mode sensor applications.

  9. Variable speed and constant frequency control of hydraulic wind turbine with energy storage system

    National Research Council Canada - National Science Library

    Liu, Zengguang; Yang, Guolai; Wei, Liejiang; Yue, Daling

    2017-01-01

    ... hydraulic system of hydraulic wind turbine for the first time. The whole hydraulic system consists of a fixed displacement pump, a variable displacement motor, two proportional control valves and an energy storage system...

  10. Estimation of the high-spatial-resolution variability in extreme wind speeds for forestry applications

    Science.gov (United States)

    Venäläinen, Ari; Laapas, Mikko; Pirinen, Pentti; Horttanainen, Matti; Hyvönen, Reijo; Lehtonen, Ilari; Junila, Päivi; Hou, Meiting; Peltola, Heli M.

    2017-07-01

    The bioeconomy has an increasing role to play in climate change mitigation and the sustainable development of national economies. In Finland, a forested country, over 50 % of the current bioeconomy relies on the sustainable management and utilization of forest resources. Wind storms are a major risk that forests are exposed to and high-spatial-resolution analysis of the most vulnerable locations can produce risk assessment of forest management planning. In this paper, we examine the feasibility of the wind multiplier approach for downscaling of maximum wind speed, using 20 m spatial resolution CORINE land-use dataset and high-resolution digital elevation data. A coarse spatial resolution estimate of the 10-year return level of maximum wind speed was obtained from the ERA-Interim reanalyzed data. Using a geospatial re-mapping technique the data were downscaled to 26 meteorological station locations to represent very diverse environments. Applying a comparison, we find that the downscaled 10-year return levels represent 66 % of the observed variation among the stations examined. In addition, the spatial variation in wind-multiplier-downscaled 10-year return level wind was compared with the WAsP model-simulated wind. The heterogeneous test area was situated in northern Finland, and it was found that the major features of the spatial variation were similar, but in some locations, there were relatively large differences. The results indicate that the wind multiplier method offers a pragmatic and computationally feasible tool for identifying at a high spatial resolution those locations with the highest forest wind damage risks. It can also be used to provide the necessary wind climate information for wind damage risk model calculations, thus making it possible to estimate the probability of predicted threshold wind speeds for wind damage and consequently the probability (and amount) of wind damage for certain forest stand configurations.

  11. Estimation of the high-spatial-resolution variability in extreme wind speeds for forestry applications

    Directory of Open Access Journals (Sweden)

    A. Venäläinen

    2017-07-01

    Full Text Available The bioeconomy has an increasing role to play in climate change mitigation and the sustainable development of national economies. In Finland, a forested country, over 50 % of the current bioeconomy relies on the sustainable management and utilization of forest resources. Wind storms are a major risk that forests are exposed to and high-spatial-resolution analysis of the most vulnerable locations can produce risk assessment of forest management planning. In this paper, we examine the feasibility of the wind multiplier approach for downscaling of maximum wind speed, using 20 m spatial resolution CORINE land-use dataset and high-resolution digital elevation data. A coarse spatial resolution estimate of the 10-year return level of maximum wind speed was obtained from the ERA-Interim reanalyzed data. Using a geospatial re-mapping technique the data were downscaled to 26 meteorological station locations to represent very diverse environments. Applying a comparison, we find that the downscaled 10-year return levels represent 66 % of the observed variation among the stations examined. In addition, the spatial variation in wind-multiplier-downscaled 10-year return level wind was compared with the WAsP model-simulated wind. The heterogeneous test area was situated in northern Finland, and it was found that the major features of the spatial variation were similar, but in some locations, there were relatively large differences. The results indicate that the wind multiplier method offers a pragmatic and computationally feasible tool for identifying at a high spatial resolution those locations with the highest forest wind damage risks. It can also be used to provide the necessary wind climate information for wind damage risk model calculations, thus making it possible to estimate the probability of predicted threshold wind speeds for wind damage and consequently the probability (and amount of wind damage for certain forest stand configurations.

  12. Frequency support capability of variable speed wind turbine based on electromagnetic coupler

    DEFF Research Database (Denmark)

    You, Rui; Barahona Garzón, Braulio; Chai, Jianyun

    2015-01-01

    frequency which is the input signal for Type 3 and Type 4 wind turbine frequency support controller, is used for the calculation of WT-EMC supplementary torque command. The integrated simulation environment based on the aeroelastic code HAWC2 and software Matlab/Simulink is used to build a 2 MW WT-EMC model...... capability has to be enhanced. In this paper, the frequency support capability of WT-EMC is studied at three typical wind conditions and with two control strategies-droop control and inertial control to enhance its frequency support capability. The synchronous generator speed, more stable than the grid...

  13. VALIDITY OF SINGLE VARIABLES AND COMPOSITE INDEXES FOR MEASURING DISEASE-ACTIVITY IN RHEUMATOID-ARTHRITIS

    NARCIS (Netherlands)

    VANDERHEIJDE, DMFM; VANTHOF, MA; VANRIEL, PLCM; VANLEEUWEN, MA; VANRIJSWIJK, MH; VANDEPUTTE, LBA

    There is no agreement as to which variable best mirrors disease activity in rheumatoid arthritis (RA) and no studies have been performed on the validity of disease activity variables. In this study the validity of 10 commonly used single variables and three composite indices was tested. All patients

  14. Modelling and Design of a 3 kW Permanent Magnet Synchronous Generator suitable for Variable Speed Small Wind Turbines

    Directory of Open Access Journals (Sweden)

    Acharya Parash

    2016-01-01

    Full Text Available This paper presents the modeling and design of a 3 kW Permanent Magnet Synchronous Generator (PMSG used for a variable speed wind turbine. Initially, the PMSG is modeled in the d-q reference frame. Different optimized parameters of the generator are extracted from the design and used in simulation of the PMSG. The generator output power is matched with the power of the turbine such that the generator is not either over-sized or under-sized.

  15. Using LDR as Sensing Element for an External Fuzzy Controller Applied in Photovoltaic Pumping Systems with Variable-Speed Drives

    Science.gov (United States)

    Maranhão, Geraldo Neves De A.; Brito, Alaan Ubaiara; Leal, Anderson Marques; Fonseca, Jéssica Kelly Silva; Macêdo, Wilson Negrão

    2015-01-01

    In the present paper, a fuzzy controller applied to a Variable-Speed Drive (VSD) for use in Photovoltaic Pumping Systems (PVPS) is proposed. The fuzzy logic system (FLS) used is embedded in a microcontroller and corresponds to a proportional-derivative controller. A Light-Dependent Resistor (LDR) is used to measure, approximately, the irradiance incident on the PV array. Experimental tests are executed using an Arduino board. The experimental results show that the fuzzy controller is capable of operating the system continuously throughout the day and controlling the direct current (DC) voltage level in the VSD with a good performance. PMID:26402688

  16. Performance analysis of variable speed multiphase induction motor with pole phase modulation

    Directory of Open Access Journals (Sweden)

    Liu Huijuan

    2016-09-01

    Full Text Available The pole phase modulation (PPM technique is an effective method to extend speed range and torque capabilities for an integrated starter and hybrid electric vehicles applications. In this paper, the five pole-phase combination types of a multiphase induction motor (IM with 36 stator slots and 36 stator conductors are presented and compared quantitatively by using the time-stepping finite element method (TS-FEM. The 36 stator conductors of the proposed multiphase IM are fed by a 36 leg inverter and the current phase angle and amplitude of each stator conductor can be controlled independently. This paper focuses on the winding connection, the PPM technique and the performance comparative analysis of each pole-phase combination types of the proposed multiphase IM. The flux distribution, air-gap flux density, output torque, core losses and efficiency of five pole-phase combination types have been investigated.

  17. An Energy Efficient Hydraulic Winch Drive Concept Based on a Speed-variable Switched Differential Pump

    DEFF Research Database (Denmark)

    Schmidt, Lasse; Andersen, Torben O.; Pedersen, Henrik Clemmensen

    2017-01-01

    The application of valve driven hydraulic winch drives is related to substantial power losses, primarily due to throttle generated valve flows. More energy efficient solutions are also commonly applied in terms of conventional hydrostatic closed circuit drives as well as so-called secondary......, originally designed for direct drive of hydraulic differential cylinders. This concept utilizes three pumps, driven by a single electric servo drive. The concept is redesigned for usage in winch drives, driven by flow symmetric hydraulic motors and single directional loads as commonly seen in e.g. active...

  18. High-Speed Single Quantum Dot Imaging of Artificial Lipids in Live Cells Reveal Partial Hop Diffusion

    DEFF Research Database (Denmark)

    Lagerholm, B. Christoffer; Clausen, Mathias P.; Christensen, Eva Arnspang

    2011-01-01

    Ultra high-speed single particle tracking (image frame rates 40-50,000 Hz) experiments with 40 nm gold particles has indicated that lipids and proteins in the plasma membrane undergo hop-diffusion between nanometer sized compartments (Fujiwara et al. (2002) J Cell Biol. 157: 1071......-81). These findings have yet to be independently confirmed. In this work, we show that high-speed single particle tracking with quantum dots(QDs)and using a standard wide-field fluorescence microscope and an EMCCD is possible at image acquisition rates of up to ~2000 Hz with an image integration time of ~0.5 msec....... The spatial precision in these experiments is ~40 nm (as determined from the standard deviation of repeated position measurements of an immobile QD on a cell). Using this system, we further show that an artificial lipid, biotin-cap-DPPE, inserted in a mouse embryo fibroblast (MEF), labeled with sAv-QD655...

  19. An Electro-Thermal Analysis of a Variable-Speed Doubly-Fed Induction Generator in a Wind Turbine

    Directory of Open Access Journals (Sweden)

    Yingning Qiu

    2015-04-01

    Full Text Available This paper focuses on the electro-thermal analysis of a doubly-fed induction generator (DFIG in a wind turbine (WT with gear transmission configuration. The study of the thermal mechanism plays an important role in the development of cost-effective fault diagnostic techniques, design for reliability and premature failure prevention. Starting from an analysis of the DFIG system control and its power losses mechanism, a model that synthesizes the thermal mechanism of the DFIG and a WT system principle is developed to study the thermodynamics of generator stator winding. The transient-state and steady-state temperature characteristics of stator winding under constant and step-cycle patterns of wind speed are studied to show an intrinsic thermal process within a variable-speed WT generator. Thermal behaviors of two failure modes, i.e., generator ventilation system failure and generator stator winding under electric voltage unbalance, are examined in details and validated by both simulation and data analysis. The effective approach presented in this paper for generator fault diagnosis using the acquired SCADA data shows the importance of simulation models in providing guidance for post-data analysis and interpretation. WT generator winding lifetime is finally estimated based on a thermal ageing model to investigate the impacts of wind speed and failure mode.

  20. Evaluation of different inertial control methods for variable-speed wind turbines simulated by fatigue, aerodynamic, structures and turbulence (FAST)

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Xiao; Gao, Wenzhong; Scholbrock, Andrew; Muljadi, Eduard; Gevorgian, Vahan; Wang, Jianhui; Yan, Weihang; Zhang, Huaguang

    2017-10-18

    To mitigate the degraded power system inertia and undesirable primary frequency response caused by large-scale wind power integration, the frequency support capabilities of variable-speed wind turbines is studied in this work. This is made possible by controlled inertial response, which is demonstrated on a research turbine - controls advanced research turbine, 3-bladed (CART3). Two distinct inertial control (IC) methods are analysed in terms of their impacts on the grids and the response of the turbine itself. The released kinetic energy in the IC methods are determined by the frequency measurement or shaped active power reference in the turbine speed-power plane. The wind turbine model is based on the high-fidelity turbine simulator fatigue, aerodynamic, structures and turbulence, which constitutes the aggregated wind power plant model with the simplified power converter model. The IC methods are implemented over the baseline CART3 controller, evaluated in the modified 9-bus and 14-bus testing power grids considering different wind speeds and different wind power penetration levels. The simulation results provide various insights on designing such kinds of ICs. The authors calculate the short-term dynamic equivalent loads and give a discussion about the turbine structural loadings related to the inertial response.

  1. Accurate prediction of complex free surface flow around a high speed craft using a single-phase level set method

    Science.gov (United States)

    Broglia, Riccardo; Durante, Danilo

    2017-11-01

    This paper focuses on the analysis of a challenging free surface flow problem involving a surface vessel moving at high speeds, or planing. The investigation is performed using a general purpose high Reynolds free surface solver developed at CNR-INSEAN. The methodology is based on a second order finite volume discretization of the unsteady Reynolds-averaged Navier-Stokes equations (Di Mascio et al. in A second order Godunov—type scheme for naval hydrodynamics, Kluwer Academic/Plenum Publishers, Dordrecht, pp 253-261, 2001; Proceedings of 16th international offshore and polar engineering conference, San Francisco, CA, USA, 2006; J Mar Sci Technol 14:19-29, 2009); air/water interface dynamics is accurately modeled by a non standard level set approach (Di Mascio et al. in Comput Fluids 36(5):868-886, 2007a), known as the single-phase level set method. In this algorithm the governing equations are solved only in the water phase, whereas the numerical domain in the air phase is used for a suitable extension of the fluid dynamic variables. The level set function is used to track the free surface evolution; dynamic boundary conditions are enforced directly on the interface. This approach allows to accurately predict the evolution of the free surface even in the presence of violent breaking waves phenomena, maintaining the interface sharp, without any need to smear out the fluid properties across the two phases. This paper is aimed at the prediction of the complex free-surface flow field generated by a deep-V planing boat at medium and high Froude numbers (from 0.6 up to 1.2). In the present work, the planing hull is treated as a two-degree-of-freedom rigid object. Flow field is characterized by the presence of thin water sheets, several energetic breaking waves and plungings. The computational results include convergence of the trim angle, sinkage and resistance under grid refinement; high-quality experimental data are used for the purposes of validation, allowing to

  2. Design and Analysis of an Axially Laminated Reluctance Motor for Variable-Speed Applications

    OpenAIRE

    BESER, E. K.; CAMUR, S.; ARIFOGLU, B.; BESER, E.

    2013-01-01

    In this paper, an axially laminated reluctance motor is presented. First, a set of a finite element analysis (FEA) on three different axially laminated rotor geometries was carried out and torque profiles of the rotors were predicted. The effect of the stator slot skewing on the torque profiles were also examined in the analysis. After deciding the rotor geometry, the mathematical model of the proposed motor was formed in terms of a,b,c variables and simulations were performed. Motor prot...

  3. Variable-speed belt conveyor systems improve economy in operation; Drehzahlgeregelte Bandanlagen verbessern die betriebswirtschaftlichen Kennzahlen

    Energy Technology Data Exchange (ETDEWEB)

    Keller, C.; Neuffer, I.; Weber, D. [Siemens AG, Erlangen (Germany). Bereich Anlagenbau und Technische Dienstleistungen (ATD)

    1999-03-01

    Previous large conveyor system solutions were a compromise between mechanical design and electrical drive technology, usually based on slipring motors for cost reasons. Today`s power electronics systems now make it possible to use medium-voltage frequency converters in large conveyor systems. This new technology opens up design solutions with torque control that yield distinctly better behaviour under load fluctuation conditions, thereby reducing wear and tear. Experience gained from completed projects has shown that a higher-level load-dependent conveyor speed control can achieve energy savings of up to 20%, since material flows tend to be stochastic rather than constant. Maximum speed is required for short periods only and can therefore be obtained by means of field weakening, a higher gear ratio and short-time overshooting of the rated motor current. This permits the use of motors with smaller frame sizes. Furthermore, in view of the controlled torque and shorter belt runs, it is conceivable that built-in safety margins could be reduced. The solution presented here shows how a distinct improvement in cost efficiency can be attained. (orig.) [Deutsch] Bisherige Loesungen fuer grosse Bandanlagen sind ein Kompromiss zwischen mechanischer Konstruktion und elektrischer Antriebstechnik, bei der aus Kostengruenden meist Schleifringlaeufermotoren eingesetzt werden. Aufgrund der Moeglichkeiten, die die Leistungselektronik heute bietet, ist ein Einsatz von Mittelspannungsumrichtern auch auf diesem Gebiet realisierbar. Damit sind Loesungen moeglich, die besonders durch ein gefuehrtes Moment ein deutlich verbessertes Verhalten bei Momenten- oder Lastaenderungen herbeifuehren und damit eine materialschonende Betriebsweise ermoeglichen. Ausserdem hat sich bei ausgefuehrten Anlagen gezeigt, dass sich durch eine uebergeordnete foerderleistungsabhaengige Bandgeschwindigkeitsregelung auch Energie bis zu 20% einsparen laesst, da der Materialfluss nicht stetig, sondern eher

  4. able utilizando redes neuronales artificiales; UTILIZATION OF ARTIFICIAL NEURAL NETWORK IN THE SIMULATION AND CONTROL OF WIND TURBINE GENERATORS WITH VARIABLE SPEED AND VARIABLE PITCH.

    Directory of Open Access Journals (Sweden)

    Osley López González

    2011-02-01

    , considered as a whole, must be able of respond with anadequate precision and speed in response to the randomness and variability of the wind.The relationship between the wind speed, the blade pitch and the generator speed in order to produce themaximum power and also be able to limit the output power for large wind speeds is a very complicated oneand it is very difficult to find its mathematical function.In this paper, the authors, utilizing the MATLABSIMULINK toolboxes, propose representing this functional relation by means of an Artificial Neural Network(ANN. The parameters and characteristics of an existing wind turbine generator are utilized and it isdemonstrated that it is possible to use an ANN in the simulation and control of a variable speed, variablepitch wind turbine that capture the maximum power from the wind.

  5. An Energy Efficient Hydraulic Winch Drive Concept Based on a Speed-variable Switched Differential Pump

    DEFF Research Database (Denmark)

    Schmidt, Lasse; Andersen, Torben O.; Pedersen, Henrik Clemmensen

    2017-01-01

    , originally designed for direct drive of hydraulic differential cylinders. This concept utilizes three pumps, driven by a single electric servo drive. The concept is redesigned for usage in winch drives, driven by flow symmetric hydraulic motors and single directional loads as commonly seen in e.g. active......The application of valve driven hydraulic winch drives is related to substantial power losses, primarily due to throttle generated valve flows. More energy efficient solutions are also commonly applied in terms of conventional hydrostatic closed circuit drives as well as so-called secondary...... heave compensation applications. A general drive configuration approach is presented, along with a proper control strategy and design. The resulting concept is evaluated when applied for active heave compensation. Results demonstrate control performance on level with conventional valve solutions...

  6. Evaluation of the Inertial Response of Variable-Speed Wind Turbines Using Advanced Simulation: Preprint

    Energy Technology Data Exchange (ETDEWEB)

    Scholbrock, Andrew K [National Renewable Energy Laboratory (NREL), Golden, CO (United States); Muljadi, Eduard [National Renewable Energy Laboratory (NREL), Golden, CO (United States); Gevorgian, Vahan [National Renewable Energy Laboratory (NREL), Golden, CO (United States); Scholbrock, Andrew K [National Renewable Energy Laboratory (NREL), Golden, CO (United States); Wang, Xiao [Northeastern University; Gao, Wenzhong [University of Denver; Yan, Weihang [University of Denver; Wang, Jianhui [Northeastern University

    2017-08-09

    In this paper, we focus on the temporary frequency support effect provided by wind turbine generators (WTGs) through the inertial response. With the implemented inertial control methods, the WTG is capable of increasing its active power output by releasing parts of the stored kinetic energy when the frequency excursion occurs. The active power can be boosted temporarily above the maximum power points, but the rotor speed deceleration follows and an active power output deficiency occurs during the restoration of rotor kinetic energy. In this paper, we evaluate and compare the inertial response induced by two distinct inertial control methods using advanced simulation. In the first stage, the proposed inertial control methods are analyzed in offline simulation. Using an advanced wind turbine simulation program, FAST with TurbSim, the response of the researched wind turbine is comprehensively evaluated under turbulent wind conditions, and the impact on the turbine mechanical components are assessed. In the second stage, the inertial control is deployed on a real 600-kW wind turbine, the three-bladed Controls Advanced Research Turbine, which further verifies the inertial control through a hardware-in-the-loop simulation. Various inertial control methods can be effectively evaluated based on the proposed two-stage simulation platform, which combines the offline simulation and real-time hardware-in-the-loop simulation. The simulation results also provide insights in designing inertial control for WTGs.

  7. Structural dynamics of single molecules studied with high-speed atomic force microscopy.

    Science.gov (United States)

    Henderson, Robert M

    2015-03-01

    Atomic force microscopy (AFM) is a scanning probe technique that has been in use in biology to generate sub-nanometre resolution images in near-physiological environments for over 20 years. Most AFM work uses instruments that take several minutes to generate each image but instruments that can produce real-time images have recently become available and there is now a reasonable body of work published on this technique. The importance of this high-speed AFM is that dynamic events of individual macromolecules can be studied. This review focuses on specific examples that demonstrate the potential of the technique. It covers four areas in which high-speed AFM has been used to elucidate mechanisms that are either unstudied or not clearly understood. These areas are: protein-protein interactions; DNA-protein interactions; quantification of biological processes; the use of DNA origami scaffolds as nanostructures to build and study dynamic molecular events. High-speed AFM shares advantages and disadvantages with conventional AFM, but it compares well in quality of data generated and in ease of use with other currently available techniques of high-resolution biological imaging. As the instruments become more widespread, the value of high-speed AFM and its potential to complement other techniques in molecular and cell biology should become more appreciated.

  8. Motion stability of high-speed maglev systems in consideration of aerodynamic effects: a study of a single magnetic suspension system

    Science.gov (United States)

    Wu, Han; Zeng, Xiao-Hui; Yu, Yang

    2017-12-01

    In this study, the intrinsic mechanism of aerodynamic effects on the motion stability of a high-speed maglev system was investigated. The concept of a critical speed for maglev vehicles considering the aerodynamic effect is proposed. The study was carried out based on a single magnetic suspension system, which is convenient for proposing relevant concepts and obtaining explicit expressions. This study shows that the motion stability of the suspension system is closely related to the vehicle speed when aerodynamic effects are considered. With increases of the vehicle speed, the stability behavior of the system changes. At a certain vehicle speed, the stability of the system reaches a critical state, followed by instability. The speed corresponding to the critical state is the critical speed. Analysis reveals that when the system reaches the critical state, it takes two forms, with two critical speeds, and thus two expressions for the critical speed are obtained. The conditions of the existence of the critical speed were determined, and the effects of the control parameters and the lift coefficient on the critical speed were analyzed by numerical analysis. The results show that the first critical speed appears when the aerodynamic force is upward, and the second critical speed appears when the aerodynamic force is downward. Moreover, both critical speeds decrease with the increase of the lift coefficient.

  9. Are there different factors affecting walking speed and gait cycle variability between men and women in community-dwelling older adults?

    Science.gov (United States)

    Inoue, Wakako; Ikezoe, Tome; Tsuboyama, Tadao; Sato, Ikuya; Malinowska, Katarzyna Bronislawa; Kawaguchi, Takahisa; Tabara, Yasuharu; Nakayama, Takeo; Matsuda, Fumihiko; Ichihashi, Noriaki

    2017-04-01

    Few studies have examined the relationships between walking speed and gait cycle variability, and muscle strength and postural stability, with a focus on gender differences. The aim of this study was to examine whether there are different factors affecting walking speed and gait cycle variability between men and women in community-dwelling older adults. The subjects comprised 712 community-dwelling older adults (252 men, 460 women, aged 68.7 ± 4.8 years). Walking speed and coefficient of variation (CV) of step time at a comfortable walking pace were measured. The maximal isometric strength of six lower limb muscles and postural stability were evaluated. Stepwise regression analysis was performed, using lower limb muscle strength and postural stability as independent variables, to investigate the association with walking speed or CV. For older men, age, body mass index (BMI) and quadriceps setting (QS) strength were significant and independent determinants of walking speed. No variables were identified as significant determinants of CV. For older women, BMI and hip flexion, hip abduction, QS muscle strength were significant determinants of walking speed. Only hip abduction strength was a significant determinant of CV. The results of this study suggest that QS strength is related to walking speed in both men and women, whereas hip flexion and abduction muscle strength are related to walking speed, and hip abduction muscle strength is related to gait cycle variability in older women. Gender differences exist in factors affecting walking speed and gait cycle variability in community-dwelling older adults.

  10. The DC Motor Speed Controller Using AT89S52 Microcontroller to Rotate Stepper Motor Attached into Potentiometer in Variable Regulated Power Supply

    Directory of Open Access Journals (Sweden)

    Marhaposan Situmorang

    2011-06-01

    Full Text Available The DC motor speed controller using AT89S52 microcontroller with stepper motor attached into potentiometer in variable regulated power supply had been evaluated. The voltage across DC motor is varied using program subroutine in microcontroller. The reference speed was determined using keypad and actual speed measured using rotating disc with holes in optocoupler sensor. The actual speed in rpm was determined after running time base 1 second and substracted with reference speed. The error was used to turn right stepper motor if actual speed less than reference speed and vice versa. The number of step of stepper motor rotation in one cycle execution was varied using subroutine starting from 1 step, 3 step, 5 step and using approximation of difference value between actual speed and reference speed. It was observed that the best performance of controller was achieved if number of step of turning stepper motor was not constant but depending on the difference between actual speed and reference speed.

  11. Comparative Analysis of Decoupling Control Methodologies and H∞ Multivariable Robust Control for Variable-Speed, Variable-Pitch Wind Turbines: Application to a Lab-Scale Wind Turbine

    Directory of Open Access Journals (Sweden)

    Sergio Fragoso

    2017-04-01

    Full Text Available This work is focused on the improvement of variable-speed variable-pitch wind turbine performance by means of its control structure. This kind of systems can be considered as multivariable nonlinear processes subjected to undesired interactions between variables and presenting different dynamics at different operational zones. This interaction level and the dynamics uncertainties complicate the control system design. The aim of this work is developing multivariable controllers that cope with such problems. The study shows the applicability of different decoupling methodologies and provides a comparison with a H∞ controller, which is an appropriate strategy to cope with uncertainties. The methodologies have been tested in simulation and verified experimentally in a lab-scale wind turbine. It is demonstrated that the wind turbine presents more interaction at the transition zone. Then, this operational point is used as the nominal one for the controller designs. At this point, decoupling controllers obtain perfect decoupling while the H∞ control presents important interaction in the generated power loop. On the other hand, they are slightly surpassed by the robust design at other points, where perfect decoupling is not achieved. However, decoupling controllers are easier to design and implement, and specifically dynamic simplified decoupling achieve the best global response. Then, it is concluded that the proposed methodologies can be considered for implantation in industrial wind turbines to improve their performance.

  12. Fluctuations Mitigation of Variable Speed Wind Turbine through Optimized Centralized Controller

    Directory of Open Access Journals (Sweden)

    Saeed Tavakoli

    2012-12-01

    Full Text Available A wind energy conversion system (WECS including a variable wind turbine in grid-connected mode is considered to control. In this paper, each component of WECS model is systematically presented and then the integrated overall model is validated.Regarding to nonlinear nature of WECS and the complex system structure as multiple-input- multiple-output (MIMO,control procedure counters the problem which strategy to handle.To simplify the control policy, a centralized controller which is compatible with systematic modelling introduced, is employed. In other hand to augment the centralized controller performance, an optimization based on genetic algorithm (GA is accomplished. Simulation results demonstate the proper efficiency in fluctuations mitigation.

  13. Intercommunications of indexes of speed and power qualities of sportsmen single combat on the stage of the specialized base preparation

    Directory of Open Access Journals (Sweden)

    Nykytenko A.O.

    2013-01-01

    Full Text Available The purpose of work is a study of interdependence between indexes specially-preparatory and general preparatory exercises of sportsmen (boxing, sporting hand-to-hand fight hopak, fencing. 42 sportsmen took part in researches. Statistical reliable intercommunications are set: a indexes of force of shots by hands with the indexes of rate of single movement; b indexes of force of shots by feet with indexes from maximal force of muscles (tractive forces; c indexes of speed of shots by hands (pricking with the indexes of distance of shove of balls by mass 300 gramme; d indexes of speed of movement a step in a battle bar with indexes: speeds of shots by hands (pricking; e indexes of frequency of shots of boxers and sportsmen hand-to-hand fight hopak with the indexes of frequency of movement (manoeuvring on feet. Facilities which it is expedient to apply in the training process of single combats on this stage of long-term preparation are certain.

  14. Steady State Analysis Of The Variable Speed Switched Reluctance Motor Drive

    Science.gov (United States)

    Materu, P.; Krishnan, R.; Farzanehfard, H.

    1987-10-01

    The switched reluctance motor (SRM) drive has recently received attention mainly because of its simple motor construction and unidirectional converter requirement. The principle of operation of the motor drive demands that the motor and converter be treated as one unit. Little has been done to develop a complete analysis of this motor-converter combination 1'2. This paper presents an approach to the steady state analysis of the SRM drive including the effects of stator winding resistance, input filter dynamics and snubber circuits which are often neglected. The analysis yields phase current waveforms providing guidelines to the optimal design of the converter and motor. A novel single-switch-per phase converter developed by one of the authors is used. The approach can be used for any other motor-converter combination.

  15. Step-to-step spatiotemporal variables and ground reaction forces of intra-individual fastest sprinting in a single session.

    Science.gov (United States)

    Nagahara, Ryu; Mizutani, Mirai; Matsuo, Akifumi; Kanehisa, Hiroaki; Fukunaga, Tetsuo

    2017-10-07

    We aimed to investigate the step-to-step spatiotemporal variables and ground reaction forces during the acceleration phase for characterising intra-individual fastest sprinting within a single session. Step-to-step spatiotemporal variables and ground reaction forces produced by 15 male athletes were measured over a 50-m distance during repeated (three to five) 60-m sprints using a long force platform system. Differences in measured variables between the fastest and slowest trials were examined at each step until the 22nd step using a magnitude-based inferences approach. There were possibly-most likely higher running speed and step frequency (2nd to 22nd steps) and shorter support time (all steps) in the fastest trial than in the slowest trial. Moreover, for the fastest trial there were likely-very likely greater mean propulsive force during the initial four steps and possibly-very likely larger mean net anterior-posterior force until the 17th step. The current results demonstrate that better sprinting performance within a single session is probably achieved by 1) a high step frequency (except the initial step) with short support time at all steps, 2) exerting a greater mean propulsive force during initial acceleration, and 3) producing a greater mean net anterior-posterior force during initial and middle acceleration.

  16. Speed Sensorless vector control of parallel-connected three-phase two-motor single-inverter drive system

    DEFF Research Database (Denmark)

    Gunabalan, Ramachandiran; Sanjeevikumar, Padmanaban; Blaabjerg, Frede

    2016-01-01

    This paper presents the characteristic behavior of direct vector control of two induction motors with sensorless speed feedback having the same rating parameters, paralleled combination, and supplied from a single current-controlled pulse-width-modulated voltage-source inverter drive. Natural...... as average and difference. Rotor flux is maintained constant by the rotor flux control scheme with feedback, and the estimation of rotor angle is carried out by the direct vector control technique. Both balanced and unbalanced load conditions are investigated for the proposed AC motor drive system...... observer design technique is known for its simple construction, which estimates the speed and rotor fluxes. Load torque is estimated by load torque adaptation and the average rotor flux was maintained constant by rotor flux feedback control. The technique’s convergence rate is very fast and is robust...

  17. Intrinsic imperfection of self-differencing single-photon detectors harms the security of high-speed quantum cryptography systems

    Science.gov (United States)

    Jiang, Mu-Sheng; Sun, Shi-Hai; Tang, Guang-Zhao; Ma, Xiang-Chun; Li, Chun-Yan; Liang, Lin-Mei

    2013-12-01

    Thanks to the high-speed self-differencing single-photon detector (SD-SPD), the secret key rate of quantum key distribution (QKD), which can, in principle, offer unconditionally secure private communications between two users (Alice and Bob), can exceed 1 Mbit/s. However, the SD-SPD may contain loopholes, which can be exploited by an eavesdropper (Eve) to hack into the unconditional security of the high-speed QKD systems. In this paper, we analyze the fact that the SD-SPD can be remotely controlled by Eve in order to spy on full information without being discovered, then proof-of-principle experiments are demonstrated. Here, we point out that this loophole is introduced directly by the operating principle of the SD-SPD, thus, it cannot be removed, except for the fact that some active countermeasures are applied by the legitimate parties.

  18. Increased single-photon emission computed tomography image processing speed achieved in personal computers with memory-intensive algorithms.

    Science.gov (United States)

    Pratt, J P; Lear, J L

    1993-11-01

    Recent dramatic reductions in the cost of computer random access memory (RAM) and the ability of newer microprocessors and associated personal computer operating systems to address large amounts of memory make novel strategies for high-speed image processing possible. We developed image processing algorithms that use this newly available memory to achieve increases in effective processing speed. These algorithms rely on the use of precomputed lookup tables to avoid repeated use of relatively expensive machine instructions, such as multiplications and divisions. Programs using this strategy to perform single photon emission computer tomography (SPECT) analysis were written in C and assembly language and tested on a Macintosh Quadra 950 (Apple Computer, Cupertino, CA) having 64 megabytes of RAM. The measured processing times are competitive with most dedicated nuclear medicine computers. A general implementation of such programs will allow personal computers to compete with dedicated imaging systems, at a substantial reduction in cost.

  19. Complementary Aerodynamic Performance Datasets for Variable Speed Power Turbine Blade Section from Two Independent Transonic Turbine Cascades

    Science.gov (United States)

    Flegel, Ashlie B.; Welch, Gerard E.; Giel, Paul W.; Ames, Forrest E.; Long, Jonathon A.

    2015-01-01

    Two independent experimental studies were conducted in linear cascades on a scaled, two-dimensional mid-span section of a representative Variable Speed Power Turbine (VSPT) blade. The purpose of these studies was to assess the aerodynamic performance of the VSPT blade over large Reynolds number and incidence angle ranges. The influence of inlet turbulence intensity was also investigated. The tests were carried out in the NASA Glenn Research Center Transonic Turbine Blade Cascade Facility and at the University of North Dakota (UND) High Speed Compressible Flow Wind Tunnel Facility. A large database was developed by acquiring total pressure and exit angle surveys and blade loading data for ten incidence angles ranging from +15.8deg to -51.0deg. Data were acquired over six flow conditions with exit isentropic Reynolds number ranging from 0.05×106 to 2.12×106 and at exit Mach numbers of 0.72 (design) and 0.35. Flow conditions were examined within the respective facility constraints. The survey data were integrated to determine average exit total-pressure and flow angle. UND also acquired blade surface heat transfer data at two flow conditions across the entire incidence angle range aimed at quantifying transitional flow behavior on the blade. Comparisons of the aerodynamic datasets were made for three "match point" conditions. The blade loading data at the match point conditions show good agreement between the facilities. This report shows comparisons of other data and highlights the unique contributions of the two facilities. The datasets are being used to advance understanding of the aerodynamic challenges associated with maintaining efficient power turbine operation over a wide shaft-speed range.

  20. Continuous Transmission Frequency Modulation Detection under Variable Sonar-Target Speed Conditions

    Directory of Open Access Journals (Sweden)

    Jun Yang

    2013-03-01

    Full Text Available As a ranging sensor, a continuous transmission frequency modulation (CTFM sonar with its ability for range finding and range profile formation works effectively under stationary conditions. When a relative velocity exists between the target and the sonar, the echo signal is Doppler-shifted. This situation causes the output of the sensor to deviate from the actual target range, thus limiting its applications to stationary conditions only. This work presents an approach for correcting such a deviation. By analyzing the Doppler effect during the propagation process, the sensor output can be corrected by a Doppler factor. To obtain this factor, a conventional CTFM system is slightly modified by adding a single tone signal with a frequency that locates out-of-sweep range of the transmitted signal. The Doppler factor can be extracted from the echo. Both verification experiments and performance tests are carried out. Results indicate the validity of the proposed approach. Moreover, ranging precision under different processing setups is discussed. For adjacent multiple targets, the discrimination ability is influenced by displacement and velocity. A discrimination boundary is provided through an analysis.

  1. Investigations of some aspects of the spray process in a single wire arc plasma spray system using high speed camera.

    Science.gov (United States)

    Tiwari, N; Sahasrabudhe, S N; Tak, A K; Barve, D N; Das, A K

    2012-02-01

    A high speed camera has been used to record and analyze the evolution as well as particle behavior in a single wire arc plasma spray torch. Commercially available systems (spray watch, DPV 2000, etc.) focus onto a small area in the spray jet. They are not designed for tracking a single particle from the torch to the substrate. Using high speed camera, individual particles were tracked and their velocities were measured at various distances from the spray torch. Particle velocity information at different distances from the nozzle of the torch is very important to decide correct substrate position for the good quality of coating. The analysis of the images has revealed the details of the process of arc attachment to wire, melting of the wire, and detachment of the molten mass from the tip. Images of the wire and the arc have been recorded for different wire feed rates, gas flow rates, and torch powers, to determine compatible wire feed rates. High speed imaging of particle trajectories has been used for particle velocity determination using time of flight method. It was observed that the ripple in the power supply of the torch leads to large variation of instantaneous power fed to the torch. This affects the velocity of the spray particles generated at different times within one cycle of the ripple. It is shown that the velocity of a spray particle depends on the instantaneous torch power at the time of its generation. This correlation was established by experimental evidence in this paper. Once the particles leave the plasma jet, their forward speeds were found to be more or less invariant beyond 40 mm up to 500 mm from the nozzle exit.

  2. Numerical Analysis of the Transient Behaviour of a Variable Speed Pump-Turbine during a Pumping Power Reduction Scenario

    Directory of Open Access Journals (Sweden)

    Giorgio Pavesi

    2016-07-01

    Full Text Available To achieve the carbon free electricity generation target for 2050, the penetration of renewable energy sources should further increase. To address the impacts of their unpredictable and intermittent characteristics on the future electricity grid, Pumped Hydro Energy Storage (PHES plants should enhance their regulation capability by extending their continuous operating range far beyond the optimal normal working range. However, for the time being, the regulation capability of the new generation of PHES, equipped with reversible pump-turbines due to their cost-effectiveness, is limited at part load by instability problems. The aim of this paper is to analyse, during a pumping power reduction scenario, the onset and development of unsteady phenomena leading to unstable behaviour. A 3D transient numerical simulation was carried out on the first stage of a variable-speed two-stage pump-turbine from full load to the unstable operating zone by progressively reducing the speed from 100% to 88% rpm corresponding to a power reduction from full load to about 60% with a ramp rate of 1.5% per s. Two three-dimensional unsteady flow structures affecting the return channel and the wicket gates at the end of the first stage were identified and their evolution in the power regulation scenario was fluid-dynamically and spectrally characterized to determine the fluid-dynamical conditions causing the head drop in the hump zone.

  3. Variable-speed systems for higher efficiencies. ALSTOM Power Conversion; Drehzahlvariable Systeme verbessern die Effizienz. ALSTOM Power Conversion

    Energy Technology Data Exchange (ETDEWEB)

    Wilke, S. [ALSTOM Power Conversion GmbH, Berlin (Germany)

    2002-07-01

    Variable-speed generators can enhance power generation efficiencies depending on the plant type and site. For example, an offshore wind power system in the North Sea can utilize stronger and more constant winds than a plant in a low inland mountain range, i.e. the demands on the plant itself will differ. A variable-speed drive is recommended which adapts the plant to the available wind and provides optimum power quality with maximum energy yield and availability. Optimum adjustment of the system components is a key prerequisite for this. The central components of the energy conversion system, i.e. the generators and current inverters, convert rotational energy of the rotors into electric power which is supplied to the public grid. Both components have been optimized continually by ALSTOM for many years and have been used successfully in many different systems. [German] Ein drehzahlgeregelter Generator ermoeglicht je nach Anlagentyp und Standort die Steigerung der Energieausbeute. Eine Offshore-Windkraftanlage in der Nordsee kann staerkere und dauerhaftere Winde als eine Anlage, die im Mittelgebirge aufgestellt ist, nutzen. Daraus ergeben sich unterschiedliche Anforderungen an die Windkraftanlage selbst. Gefragt ist eine Steuerung, die das gesamte Antriebssystem, einschliesslich der Rotorblaetter, optimal an die gegebene Windsituation anpasst. Als Loesung bietet sich ein drehzahlgeregeltes Antriebssystem an, das sich auf die jeweilige Windsituation einstellt und bei maximaler Energieausbeute und Verfuegbarkeit die bestmoegliche Stromqualitaet zur Verfuegung stellt. Eine wichtige Voraussetzung dafuer ist die optimale Abstimmung der Systemkomponenten. Die zentralen Komponenten des Energieumwandlungssystems - Generatoren und Stromrichter - sorgen in Windkraftanlagen fuer die Umwandlung der Rotationsenergie in elektrischen Storm, der ins Netz gespeist wird. Beide Komponenten werden von ALSTOM schon seit Jahren kontinuierlich optimiert und haben sich in einer Vielzahl

  4. Adaptive robust control of a class of non-affine variable-speed variable-pitch wind turbines with unmodeled dynamics.

    Science.gov (United States)

    Bagheri, Pedram; Sun, Qiao

    2016-07-01

    In this paper, a novel synthesis of Nussbaum-type functions, and an adaptive radial-basis function neural network is proposed to design controllers for variable-speed, variable-pitch wind turbines. Dynamic equations of the wind turbine are highly nonlinear, uncertain, and affected by unknown disturbance sources. Furthermore, the dynamic equations are non-affine with respect to the pitch angle, which is a control input. To address these problems, a Nussbaum-type function, along with a dynamic control law are adopted to resolve the non-affine nature of the equations. Moreover, an adaptive radial-basis function neural network is designed to approximate non-parametric uncertainties. Further, the closed-loop system is made robust to unknown disturbance sources, where no prior knowledge of disturbance bound is assumed in advance. Finally, the Lyapunov stability analysis is conducted to show the stability of the entire closed-loop system. In order to verify analytical results, a simulation is presented and the results are compared to both a PI and an existing adaptive controllers. Copyright © 2016 ISA. Published by Elsevier Ltd. All rights reserved.

  5. Analysis of the Association Between Motor and Anthropometric Variables with Change of Direction Speed and Reactive Agility Performance.

    Science.gov (United States)

    Sattler, Tine; Sekulić, Damir; Spasić, Miodrag; Perić, Mia; Krolo, Ante; Uljević, Ognjen; Kondrič, Miran

    2015-09-29

    There is an evident lack of studies examining the factors associated with reactive agility performances. The aim of this study was to evaluate the association between anthropometrics, body composition, jumping capacity, reactive strength, and balance with a stop-and-go change of direction speed (CODS) and reactive agility. The total sample comprised 39 male (body height: 182.95 ± 5.19 cm; body mass: 80.66 ± 7.69 kg) and 34 female (body height: 171.45 ± 6.81 cm; body mass: 61.95 ± 6.70 kg) college-level athletes (21.9 ± 1.9 years of age). The variables included body height, body mass, the percentage of body fat (BF%), balance as measured by an overall-stability index, the countermovement jump (CMJ), a reactive-strength index (RSI), stop-and-go reactive agility, and stop-and-go CODS. To define the associations between motor and anthropometric variables with CODS and reactive agility, the participants were clustered into three achievement groups based on their CODS and reactive agility performances. The ANOVA showed a significant difference between the CODS-based achievement groups for the CMJ (F test = 3.45 and 3.60 for males and females, respectively; p agility achievement groups differed significantly in the RSI (F test = 6.46; p agility performance in sports.

  6. High-speed atomic force microscope combined with single-molecule fluorescence microscope.

    Science.gov (United States)

    Fukuda, Shingo; Uchihashi, Takayuki; Iino, Ryota; Okazaki, Yasutaka; Yoshida, Masato; Igarashi, Kiyohiko; Ando, Toshio

    2013-07-01

    High-speed atomic force microscopy (HS-AFM) and total internal reflection fluorescence microscopy (TIRFM) have mutually complementary capabilities. Here, we report techniques to combine these microscopy systems so that both microscopy capabilities can be simultaneously used in the full extent. To combine the two systems, we have developed a tip-scan type HS-AFM instrument equipped with a device by which the laser beam from the optical lever detector can track the cantilever motion in the X- and Y-directions. This stand-alone HS-AFM system is mounted on an inverted optical microscope stage with a wide-area scanner. The capability of this combined system is demonstrated by simultaneous HS-AFM∕TIRFM imaging of chitinase A moving on a chitin crystalline fiber and myosin V walking on an actin filament.

  7. High-Speed AFM Reveals the Dynamics of Single Biomolecules at the Nanometer Scale

    NARCIS (Netherlands)

    Katan, A.J.; Dekker, C.

    2011-01-01

    Atomic force microscopy allows visualization of biomolecules with nanometer resolution under physiological conditions. Recent advances have improved the time resolution of the technique from minutes to tens of milliseconds, meaning that it is now possible to watch single biomolecules in action in

  8. High speed rail : challenges for the high speed rail project in Norway

    OpenAIRE

    Ringstad, Vidar

    2012-01-01

    This Master Thesis has focus on parts of the public transport system in Norway. The main topic in this thesis is: What variables must be calculated for the decision concerning the construction and implementation of the Norwegian High Speed Rail project, and how are the variables calculated? High Speed Rail does not have a single standard definition. High Speed Rail definition, given in the European Union definition, Directive 96/48 is suitable for many different systems of rolling stock...

  9. Low Wind Speed Turbine Project Phase II: The Application of Medium-Voltage Electrical Apparatus to the Class of Variable Speed Multi-Megawatt Low Wind Speed Turbines; 15 June 2004--30 April 2005

    Energy Technology Data Exchange (ETDEWEB)

    Erdman, W.; Behnke, M.

    2005-11-01

    Kilowatt ratings of modern wind turbines have progressed rapidly from 50 kW to 1,800 kW over the past 25 years, with 3.0- to 7.5-MW turbines expected in the next 5 years. The premise of this study is simple: The rapid growth of wind turbine power ratings and the corresponding growth in turbine electrical generation systems and associated controls are quickly making low-voltage (LV) electrical design approaches cost-ineffective. This report provides design detail and compares the cost of energy (COE) between commercial LV-class wind power machines and emerging medium-voltage (MV)-class multi-megawatt wind technology. The key finding is that a 2.5% reduction in the COE can be achieved by moving from LV to MV systems. This is a conservative estimate, with a 3% to 3.5% reduction believed to be attainable once purchase orders to support a 250-turbine/year production level are placed. This evaluation considers capital costs as well as installation, maintenance, and training requirements for wind turbine maintenance personnel. Subsystems investigated include the generator, pendant cables, variable-speed converter, and padmount transformer with switchgear. Both current-source and voltage-source converter/inverter MV topologies are compared against their low-voltage, voltage-source counterparts at the 3.0-, 5.0-, and 7.5-MW levels.

  10. Detecting single graphene layer by using fluorescence from high-speed Ar^7+ ion

    Science.gov (United States)

    Miyamoto, Yoshiyuki; Zhang, Hong

    2008-03-01

    A highly-charged-ion interacting with graphite causes structural change in nano-scales [1]. While when the ion's kinetic energy reaches few MeVs, the induced is not the structural change but electronic excitation. An experiment [2] showed fluorescence from Ar^7+ ions penetrating through carbon foil with kinetic energy of 2 MeV. Motivated by this experiment, we tested interaction between an Ar^7+ ion and a graphene sheet by the time-dependent density functional approach, and found that the electronic excitation in the Ar^ 7+ ion is also the case even when the incident kinetic energy is 500 KeV and the target thickness is only mono-atomic layer. This simulation suggests the possibility of detecting a suspended mono-atomic layer of graphene [3] by monitoring fluorescence from the penetrated Ar^7+ ions. We will discuss its importance for analyzing bombardment of solids by highly charged, high-speed ions and possible experiments according to the present result. References: [1] T. Meguro, et al., Appl. Phys. Lett 79, 3866 (2001). [2] S. Bashkin, H. Oona, E. Veje, Phys, Rev. A25, 417 (1982). [3] J. Mayer et al., Nature (London), 446, 60 (2007).

  11. Computing with a single qubit faster than the computation quantum speed limit

    Science.gov (United States)

    Sinitsyn, Nikolai A.

    2018-02-01

    The possibility to save and process information in fundamentally indistinguishable states is the quantum mechanical resource that is not encountered in classical computing. I demonstrate that, if energy constraints are imposed, this resource can be used to accelerate information-processing without relying on entanglement or any other type of quantum correlations. In fact, there are computational problems that can be solved much faster, in comparison to currently used classical schemes, by saving intermediate information in nonorthogonal states of just a single qubit. There are also error correction strategies that protect such computations.

  12. Variable dose rate single-arc IMAT delivered with a constant dose rate and variable angular spacing

    Energy Technology Data Exchange (ETDEWEB)

    Tang, Grace; Earl, Matthew A; Yu, Cedric X [Department of Radiation Oncology, University of Maryland School of Medicine, Baltimore, MD (United States)], E-mail: cyu002@umaryland.edu

    2009-11-07

    Single-arc intensity-modulated arc therapy (IMAT) has gained worldwide interest in both research and clinical implementation due to its superior plan quality and delivery efficiency. Single-arc IMAT techniques such as the Varian RapidArc(TM) deliver conformal dose distributions to the target in one single gantry rotation, resulting in a delivery time in the order of 2 min. The segments in these techniques are evenly distributed within an arc and are allowed to have different monitor unit (MU) weightings. Therefore, a variable dose-rate (VDR) is required for delivery. Because the VDR requirement complicates the control hardware and software of the linear accelerators (linacs) and prevents most existing linacs from delivering IMAT, we propose an alternative planning approach for IMAT using constant dose-rate (CDR) delivery with variable angular spacing. We prove the equivalence by converting VDR-optimized RapidArc plans to CDR plans, where the evenly spaced beams in the VDR plan are redistributed to uneven spacing such that the segments with larger MU weighting occupy a greater angular interval. To minimize perturbation in the optimized dose distribution, the angular deviation of the segments was restricted to {<=}{+-} 5 deg. This restriction requires the treatment arc to be broken into multiple sectors such that the local MU fluctuation within each sector is reduced, thereby lowering the angular deviation of the segments during redistribution. The converted CDR plans were delivered with a single gantry sweep as in the VDR plans but each sector was delivered with a different value of CDR. For four patient cases, including two head-and-neck, one brain and one prostate, all CDR plans developed with the variable spacing scheme produced similar dose distributions to the original VDR plans. For plans with complex angular MU distributions, the number of sectors increased up to four in the CDR plans in order to maintain the original plan quality. Since each sector was

  13. High interindividual variability in dose-dependent reduction in speed of movement after exposing C. elegans to shock waves

    Directory of Open Access Journals (Sweden)

    Nicholas Baker Angstman

    2015-02-01

    Full Text Available In blast-related mild traumatic brain injury (br-mTBI little is known about the connections between initial trauma and expression of individual clinical symptoms. Partly due to limitations of current in vitro and in vivo models of br-mTBI, reliable prediction of individual short- and long-term symptoms based on known blast input has not yet been possible. Here we demonstrate a dose-dependent effect of shock wave exposure on C. elegans using shock waves that share physical characteristics with those hypothesized to induce br-mTBI in humans. Increased exposure to shock waves resulted in decreased mean speed of movement while increasing the proportion of worms rendered paralyzed. Recovery of these two behavioral symptoms was observed during increasing post-traumatic waiting periods. Although effects were observed on a population-wide basis, large interindividual variability was present between organisms exposed to the same highly controlled conditions. Reduction of cavitation by exposing worms to shock waves in polyvinyl alcohol resulted in reduced effect, implicating primary blast effects as damaging components in shock wave induced trauma. Growing worms on NGM agar plates led to the same general results in initial shock wave effect in a standard medium, namely dose-dependence and high interindividual variability, as raising worms in liquid cultures. Taken together, these data indicate that reliable prediction of individual clinical symptoms based on known blast input as well as drawing conclusions on blast input from individual clinical symptoms is not feasible in br-mTBI.

  14. Utility-scale variable-speed wind turbines using a doubly-fed generator with a soft-switching power converter

    Energy Technology Data Exchange (ETDEWEB)

    Weigand, C.H.; Lauw, H.K.; Marckx, D.A. [Electronic Power Conditioning, Inc., Corvallis, OR (United States)

    1996-12-31

    Utility-scale wind turbines operating at variable RPM have been studied for a considerable period of time. Whereas the increase in energy output originally has been considered the principal benefit of variable-speed operation, the ability to tightly control the drive-train torque by electronic means is becoming another very important cost factor, especially for turbine ratings above 500 kilowatts. This cost benefit becomes even more significant as optimum turbine ratings today are approaching (and surpassing) 1 Megawatt. Having identified the benefits for the turbine, the designer is confronted with the task of finding the most cost-effective variable-speed generation system which allows him to make use of the benefits, yet does not introduce well-known electrical problems associated with state-of-the-art variable-speed generator controls, such as drastically reduced generator winding life, excessive harmonics on the utility, and poor utility power factor. This paper will indicate that for high-power (> 500 kW), utility-scale wind turbines a doubly-fed generator system in connection with a soft-switching resonant power converter is the least-cost variable-speed generation system offering all of the desired benefits, yet avoids the introduction of the potential electrical problems stated above. 3 refs., 3 figs., 1 tab.

  15. Fault-Ride through Strategy for Permanent-Magnet Synchronous Generators in Variable-Speed Wind Turbines

    Directory of Open Access Journals (Sweden)

    Mohamed Abdelrahem

    2016-12-01

    Full Text Available Currently, the electric power production by wind energy conversion systems (WECSs has increased significantly. Consequently, wind turbine (WT generators are requested to fulfill the grid code (GC requirements stated by network operators. In case of grid faults/voltage dips, a mismatch between the generated active power from the wind generator and the active power delivered to the grid is produced. The conventional approach is using a braking chopper (BC in the DC-link to dissipate this active power. This paper proposes a fault-ride through (FRT strategy for variable-speed WECSs based on permanent magnet synchronous generators (PMSGs. The proposed strategy exploits the rotor inertia of the WECS (inertia of the WT and PMSG to store the surplus active power during the grid faults/voltage dips. Thus, no additional hardware components are requested. Furthermore, a direct model predictive control (DMPC scheme for the PMSG is proposed in order to enhance the dynamic behavior of the WECS. The behavior of the proposed FRT strategy is verified and compared with the conventional BC approach for all the operation conditions by simulation results. Finally, the simulation results confirm the feasibility of the proposed FRT strategy.

  16. Validation of Generic Models for Variable Speed Operation Wind Turbines Following the Recent Guidelines Issued by IEC 61400-27

    Directory of Open Access Journals (Sweden)

    Andrés Honrubia-Escribano

    2016-12-01

    Full Text Available Considerable efforts are currently being made by several international working groups focused on the development of generic, also known as simplified or standard, wind turbine models for power system stability studies. In this sense, the first edition of International Electrotechnical Commission (IEC 61400-27-1, which defines generic dynamic simulation models for wind turbines, was published in February 2015. Nevertheless, the correlations of the IEC generic models with respect to specific wind turbine manufacturer models are required by the wind power industry to validate the accuracy and corresponding usability of these standard models. The present work conducts the validation of the two topologies of variable speed wind turbines that present not only the largest market share, but also the most technological advances. Specifically, the doubly-fed induction machine and the full-scale converter (FSC topology are modeled based on the IEC 61400-27-1 guidelines. The models are simulated for a wide range of voltage dips with different characteristics and wind turbine operating conditions. The simulated response of the IEC generic model is compared to the corresponding simplified model of a wind turbine manufacturer, showing a good correlation in most cases. Validation error sources are analyzed in detail, as well. In addition, this paper reviews in detail the previous work done in this field. Results suggest that wind turbine manufacturers are able to adjust the IEC generic models to represent the behavior of their specific wind turbines for power system stability analysis.

  17. High-speed and low-distortion solution for time-correlated single photon counting measurements: A theoretical analysis

    Science.gov (United States)

    Cominelli, A.; Acconcia, G.; Peronio, P.; Ghioni, M.; Rech, I.

    2017-12-01

    In this paper, we describe a novel solution to increase the speed of Time-Correlated Single Photon Counting (TCSPC) measurements by almost an order of magnitude while providing, in principle, zero distortion regardless of the experimental conditions. Typically, the relatively long dead time associated with the conversion electronics requires a proper tune of the excitation power in order to avoid distortions of the reconstructed waveform due to pileup and counting loss. As a result, the maximum operating rate of a TCSPC channel is now limited between 1% and 5% of the excitation frequency, thus leading to relatively long acquisition times. We show that negligible distortion (below 1%) is guaranteed if the dead time associated with the converter is kept below the dead time of the detector, and at the same time the detector dead time is matched to the duration of the excitation period. In this way, unprecedented high-speed operation is possible. In this paper, we provide a theoretical analysis of the technique, including the main non-idealities which are introduced by a generic physical implementation. The results are supported by both numerical simulations and analytical calculations.

  18. Comparison of Computational and Experimental Results for a Transonic Variable-speed Power-Turbine Blade Operating with Low Inlet Turbulence Levels

    Science.gov (United States)

    Booth, David T.; Flegel, Ashlie B.

    2015-01-01

    A computational assessment of the aerodynamic performance of the midspan section of a variable-speed power-turbine blade is described. The computation comprises a periodic single blade that represents the 2-D Midspan section VSPT blade that was tested in the NASA Glenn Research Center Transonic Turbine Blade Cascade Facility. A commercial, off-the-shelf (COTS) software package, Pointwise and CFD++, was used for the grid generation and RANS and URANS computations. The CFD code, which offers flexibility in terms of turbulence and transition modeling options, was assessed in terms of blade loading, loss, and turning against test data from the transonic tunnel. Simulations were assessed at positive and negative incidence angles that represent the turbine cruise and take-off design conditions. The results indicate that the secondary flow induced at the positive incidence cruise condition results in a highly loaded case and transitional flow on the blade is observed. The negative incidence take-off condition is unloaded and the flow is very two-dimensional. The computational results demonstrate the predictive capability of the gridding technique and COTS software for a linear transonic turbine blade cascade with large incidence angle variation.

  19. AN ALGORITHM TO MINIMIZE SINGLE VARIABLE POLYNOMIAL, FUNCTIONS FROM ANY STARTING POINT WITH QUADRATIC CONVERGENCE

    Directory of Open Access Journals (Sweden)

    James Thorne

    2012-01-01

    Full Text Available Search methods often display non-convergence or excessive convergence time on certain classes of nonlinear functions arising in engineering design. The authors will define a new geometric -programming based search method for single variable polynomials that displays quadratic convergence from any starting point Comparison over a group of test problems is made with a version of Newton's method

  20. Single-camera high-speed stereo-digital image correlation for full-field vibration measurement

    Science.gov (United States)

    Yu, Liping; Pan, Bing

    2017-09-01

    A low-cost, easy-to-implement single-camera high-speed stereo-digital image correlation (SCHS stereo-DIC) method using a four-mirror adapter is proposed for full-field 3D vibration measurement. With the aid of the four-mirror adapter, surface images of calibration target and test objects can be separately imaged onto two halves of the camera sensor through two different optical paths. These images can be further processed to retrieve the vibration responses on the specimen surface. To validate the effectiveness and accuracy of the proposed approach, dynamic parameters including natural frequencies, damping ratios and mode shapes of a rectangular cantilever plate were extracted from the directly measured vibration responses using the established system. The results reveal that the SCHS stereo-DIC is a simple, practical and effective technique for vibration measurements and dynamic parameters identification.

  1. Transient Simulation Study of Slip-Frequency Vector Control for Variable Speed Doubly-Fed Brushless Motor with Magnetic Barrier Rotor

    Directory of Open Access Journals (Sweden)

    Jingxiong ZHANG

    2014-01-01

    Full Text Available In this paper, a transient simulation model of a variable speed doubly fed brushless motor (DFBM using back-to-back converter is described. Based on analysis of rotor flux oriented vector control theory of doubly fed induction motor, the control of the currents in DFBM that produce the magnetic flux and the torque is achieved by a digital controller, the speed is regulated by a PI controller which is tuned by a genetic algorithm. According to the state equation of DFBM and the control schemes, the system simulation module is established in MATLAB/ SIMULINK. An extensive simulation study is performed to examine the control characteristics of the machine-side converter under different operation conditions in variable-speed DFBM driver system.

  2. Families of exact solutions for linear and nonlinear wave equations with a variable speed of sound and their use in solving initial boundary value problems

    Science.gov (United States)

    Trifonov, E. V.

    2017-07-01

    We propose a procedure for multiplying solutions of linear and nonlinear one-dimensional wave equations, where the speed of sound can be an arbitrary function of one variable. We obtain exact solutions. We show that the functional series comprising these solutions can be used to solve initial boundary value problems. For this, we introduce a special scalar product.

  3. Aerodynamic Measurements of a Variable-Speed Power-Turbine Blade Section in a Transonic Turbine Cascade

    Science.gov (United States)

    Flegel, Ashlie B.

    2014-01-01

    The purpose of this thesis is to document the impact of incidence angle and Reynolds number variations on the three-dimensional flow field and midspan loss and turning of a two-dimensional section of a variable-speed power-turbine (VSPT) rotor blade. Aerodynamic measurements were obtained in a transonic linear cascade at NASA Glenn Research Center in Cleveland, Ohio. Steady-state data were obtained for 10 incidence angles ranging from +15.8deg to -51.0deg. At each angle, data were acquired at five flow conditions with the exit Reynolds number (based on axial chord) varying over an order-of-magnitude from 2.12×105 to 2.12×106. Data were obtained at the design exit Mach number of 0.72 and at a reduced exit Mach number of 0.35 as required to achieve the lowest Reynolds number. Midspan tota lpressure and exit flow angle data were acquired using a five-hole pitch/yaw probe surveyed on a plane located 7.0 percent axial-chord downstream of the blade trailing edge plane. The survey spanned three blade passages. Additionally, three-dimensional half-span flow fields were examined with additional probe survey data acquired at 26 span locations for two key incidence angles of +5.8deg and -36.7deg. Survey data near the endwall were acquired with a three-hole boundary-layer probe. The data were integrated to determine average exit total-pressure and flow angle as functions of incidence and flow conditions. The data set also includes blade static pressures measured on four spanwise planes and endwall static pressures.

  4. Design Of Single-Axis And Dual-Axis Solar Tracking Systems Protected Against High Wind Speeds

    Directory of Open Access Journals (Sweden)

    Mai Salaheldin Elsherbiny

    2017-09-01

    Full Text Available Solar energy is rapidly gaining ground as an important mean of expanding renewable energy use. Solar tracking is employed in order to maximize collected solar radiation by a photovoltaic panel. In this paper we present a prototype for Automatic solar tracker that is designed using Arduino UNO with Wind sensor to Cease Wind effect on panels if wind speed exceeds certain threshold. The Proposed solar tracker tracks the location of the sun anywhere in any time by calculating the position of the sun. For producing the maximum amount of solar energy a solar panel must always be perpendicular to the source of light. Because the sun motion plane varies daily and during the day it moves from east to west one needs two axis tracking to follow the suns position. Maximum possible power is collected when two axis tracking is done. However two axis tracking is relatively costly and complex. A compromise between maximum power collection and system simplicity is obtained by single axis tracking where the plane North south axis is fixed while the east west motion is accomplished. This work deals with the design of both single and two axis tracking systems. Automatic trackers is also compared to Fixed one in terms of Energy generated Efficiency Cost and System reliability.

  5. Evaluation of single tracks of 17-4PH steel manufactured at different power densities and scanning speeds by selective laser melting

    Directory of Open Access Journals (Sweden)

    Makoana, N. W.

    2016-11-01

    Full Text Available In Selective Laser Melting, the initial units produced are single tracks that overlap to create a single layer; from the sequence of layers, a 3D object is manufactured. The properties of the parts produced by SLM depend heavily on the properties of each single track and each layer formed by these tracks. This study evaluates the effect of processing parameters on the geometrical characteristics of single tracks manufactured from 17-4PH stainless steel powder. A single-mode continuous-wave ytterbium fibre laser was used to manufacture single tracks at laser powers in the range of 100-300 W with a constant spot size of ∼80μm. The single tracks produced were subjected to standard metallographic preparation techniques for further analysis with an optical microscope. Deep molten pool shapes were observed at low scan speeds, while shallow molten pool shapes were observed at high scan speeds. At higher laser power densities, under-cutting and humping effects were also observed. The dimensions of single tracks processed without powder generally decrease with increasing scan speed at constant laser power. However, the geometrical features of the single tracks processed with powder revealed pronounced irregularities believed to be caused by non-homogeneity in the deposited powder layer.

  6. A single adaptive controller with one variable for synchronization of fractional-order chaotic systems

    Science.gov (United States)

    Zhang, Ruo-Xun; Yang, Shi-Ping

    2012-08-01

    In this paper we investigate the synchronization of a class of three-dimensional fractional-order chaotic systems. Based on the Lyapunov stability theory and adaptive control technique, a single adaptive—feedback controller is developed to synchronize a class of fractional-order chaotic systems. The presented controller which only contains a single driving variable is simple both in design and in implementation. Numerical simulation and circuit experimental results for fractional-order chaotic system are provided to illustrate the effectiveness of the proposed scheme.

  7. Graphical user interface for input output characterization of single variable and multivariable highly nonlinear systems

    Directory of Open Access Journals (Sweden)

    Shahrukh Adnan Khan M. D.

    2017-01-01

    Full Text Available This paper presents a Graphical User Interface (GUI software utility for the input/output characterization of single variable and multivariable nonlinear systems by obtaining the sinusoidal input describing function (SIDF of the plant. The software utility is developed on MATLAB R2011a environment. The developed GUI holds no restriction on the nonlinearity type, arrangement and system order; provided that output(s of the system is obtainable either though simulation or experiments. An insight to the GUI and its features are presented in this paper and example problems from both single variable and multivariable cases are demonstrated. The formulation of input/output behavior of the system is discussed and the nucleus of the MATLAB command underlying the user interface has been outlined. Some of the industries that would benefit from this software utility includes but not limited to aerospace, defense technology, robotics and automotive.

  8. An Electro-Thermal Analysis of a Variable-Speed Doubly-Fed Induction Generator in a Wind Turbine

    National Research Council Canada - National Science Library

    Qiu, Yingning; Zhang, Wenxiu; Cao, Mengnan; Feng, Yanhui; Infield, David

    2015-01-01

    ... the thermodynamics of generator stator winding. The transient-state and steady-state temperature characteristics of stator winding under constant and step-cycle patterns of wind speed are studied to show an intrinsic thermal process within...

  9. Marked and variable inhibition by chemical fixation of cytochrome oxidase and succinate dehydrogenase in single motoneurons

    Science.gov (United States)

    Chalmers, G. R.; Edgerton, V. R.

    1989-01-01

    The effect of tissue fixation on succinate dehydrogenase and cytochrome oxidase activity in single motoneurons of the rat was demonstrated using a computer image processing system. Inhibition of enzyme activity by chemical fixation was variable, with some motoneurons being affected more than others. It was concluded that quantification of enzymatic activity in chemically fixed tissue provides an imprecise estimate of enzyme activities found in fresh-frozen tissues.

  10. Single cell Hi-C reveals cell-to-cell variability in chromosome structure

    Science.gov (United States)

    Schoenfelder, Stefan; Yaffe, Eitan; Dean, Wendy; Laue, Ernest D.; Tanay, Amos; Fraser, Peter

    2013-01-01

    Large-scale chromosome structure and spatial nuclear arrangement have been linked to control of gene expression and DNA replication and repair. Genomic techniques based on chromosome conformation capture assess contacts for millions of loci simultaneously, but do so by averaging chromosome conformations from millions of nuclei. Here we introduce single cell Hi-C, combined with genome-wide statistical analysis and structural modeling of single copy X chromosomes, to show that individual chromosomes maintain domain organisation at the megabase scale, but show variable cell-to-cell chromosome territory structures at larger scales. Despite this structural stochasticity, localisation of active gene domains to boundaries of territories is a hallmark of chromosomal conformation. Single cell Hi-C data bridge current gaps between genomics and microscopy studies of chromosomes, demonstrating how modular organisation underlies dynamic chromosome structure, and how this structure is probabilistically linked with genome activity patterns. PMID:24067610

  11. High-speed measurement of single-electron circuits at low temperatures with bolometric and calorimetric applications

    Science.gov (United States)

    Swenson, Loren

    2007-12-01

    This thesis consists primarily of the description of two single-electron circuits that I fabricated and measured utilizing radio-frequency techniques. A short summary of the background material necessary for understanding this area of condensed matter experiment is included as well as a discussion of the utility of these devices as charge-sensing or energy-sensing circuit elements. In the first measurement, by configuring a radio-frequency single-electron transistor as a mixer, we demonstrate a unique implementation of this device, that achieves good charge sensitivity with large bandwidth about a tunable center frequency. In our implementation we achieve a measurement bandwidth of 16 MHz, with a tunable center frequency from 0 to 1.2 GHz, demonstrated with the transistor operating at 300 mK. Ultimately this device is limited in center frequency by the RC time of the transistor's center island, which for our device is ˜1.6 GHz, close to the measured value. The measurement bandwidth is determined by the quality factor of the readout tank circuit. In the second measurement, we detect the energy-selective diffusion of electrons through a tunnel junction to perform a new type of passive, low-power dissipation thermometry. The thermometer is based on a novel, three-junction single electron transistor, which is made with a superconducting nanoscale metal island, coupled to two tunnel junctions and a capacitive gate in the standard single-electron transistor configuration, and in addition a third tunnel junction couples the transistor island to a normal metal thin-film volume, which serves as a calorimeter. Passive diffusion of electrons from the calorimeter through the third junction changes the transistor conductance, providing a thermometric signal. This device dissipates minimal power in the calorimeter, removing a stringent limit on the minimum temperatures and energy sensitivities achievable in nanoscale calorimeters and bolometers. High speed measurements were

  12. A compact single-camera system for high-speed, simultaneous 3-D velocity and temperature measurements.

    Energy Technology Data Exchange (ETDEWEB)

    Lu, Louise; Sick, Volker; Frank, Jonathan H.

    2013-09-01

    The University of Michigan and Sandia National Laboratories collaborated on the initial development of a compact single-camera approach for simultaneously measuring 3-D gasphase velocity and temperature fields at high frame rates. A compact diagnostic tool is desired to enable investigations of flows with limited optical access, such as near-wall flows in an internal combustion engine. These in-cylinder flows play a crucial role in improving engine performance. Thermographic phosphors were proposed as flow and temperature tracers to extend the capabilities of a novel, compact 3D velocimetry diagnostic to include high-speed thermometry. Ratiometric measurements were performed using two spectral bands of laser-induced phosphorescence emission from BaMg2Al10O17:Eu (BAM) phosphors in a heated air flow to determine the optimal optical configuration for accurate temperature measurements. The originally planned multi-year research project ended prematurely after the first year due to the Sandia-sponsored student leaving the research group at the University of Michigan.

  13. Research on the speed of light transmission in a dual-frequency laser pumped single fiber with two directions

    Science.gov (United States)

    Qiu, Wei; Liu, Jianjun; Wang, Yuda; Yang, Yujing; Gao, Yuan; Lv, Pin; Jiang, Qiuli

    2018-01-01

    In this article a general theory of the coherent population oscillation effect in an erbium-doped fiber at room temperature is presented. We use dual pumping light waves with a simplified two-level system. Thus the time delay equations can be calculated from rate equations and the transmission equation. Using numerical simulation, in the case of dual-frequency pump light waves (1480 nm and 980 nm) with two directions, we analyze the influence of the pump power ratio on the group speed of light propagation. In addition, we compare slow light propagation with a single-pumping light and slow light propagation with a dual-pumping light at room temperature. The discussion shows that a larger time delay of slow light propagation can be obtained with a dual-frequency pumping laser. Compared to previous research methods, a dual-frequency laser pumped fiber with two directions is more controllable. Moreover, we conclude that the group velocity of light can be varied by changing the pump ratio.

  14. Variable speed electrical driving systems. 2, power electronics and automation recalls: the electronic speed variators; Entrainements electriques a vitesse variable. 2, rappels d`electronique de puissance et d`automatique: les variateurs electroniques

    Energy Technology Data Exchange (ETDEWEB)

    Bonal, J.; Seguier, G.

    1998-12-31

    This second volume deals first with the characteristics of power semi-conductors, their cooling and connection. Then it analyzes the structure of converters that use these semi-conductors and which corresponds to the power stage of speed variators. (J.S.)

  15. Improving the reliability of single-subject fMRI by weighting intra-run variability.

    Science.gov (United States)

    de Bertoldi, F; Finos, L; Maieron, M; Weis, L; Campanella, M; Ius, T; Fadiga, L

    2015-07-01

    At present, functional magnetic resonance imaging (fMRI) is one of the most useful methods of studying cognitive processes in the human brain in vivo, both for basic science and clinical goals. Although neuroscience studies often rely on group analysis, clinical applications must investigate single subjects (patients) only. Particularly for the latter, issues regarding the reliability of fMRI readings remain to be resolved. To determine the ability of intra-run variability (IRV) weighting to consistently detect active voxels, we first acquired fMRI data from a sample of healthy subjects, each of whom performed 4 runs (4 blocks each) of self-paced finger-tapping. Each subject's data was analyzed using single-run general linear model (GLM), and each block was then analyzed separately to calculate the IRV weighting. Results show that integrating IRV information into standard single-subject GLM activation maps significantly improved the reliability (p=0.007) of the single-subject fMRI data. This suggests that taking IRV into account can help identify the most constant and relevant neuronal activity at the single-subject level. Copyright © 2015. Published by Elsevier Inc.

  16. Video-rate fluorescence lifetime imaging camera with CMOS single-photon avalanche diode arrays and high-speed imaging algorithm

    NARCIS (Netherlands)

    Li, D.D.U.; Arlt, J.; Tyndall, D.; Walker, R.; Richardson, J.; Stoppa, D.; Charbon, E.; Henderson, R.K.

    2011-01-01

    A high-speed and hardware-only algorithm using a center of mass method has been proposed for single-detector fluorescence lifetime sensing applications. This algorithm is now implemented on a field programmable gate array to provide fast lifetime estimates from a 32 × 32 low dark count 0.13 ?m

  17. [One-step methylation variable position analysis technology in single-tube].

    Science.gov (United States)

    Yue, Yang-Yang; Zhao, Gui-Sen; Zhang, Qian; Lu, Di; Zhai, Xian-Dun; Mo, Yao-Nan

    2013-12-01

    To develop the single-tube one-step methylation variable position (MVP) analysis technology-single-tube post-digestion PCR-melting curve analysis (PDP-MCA). Based on differentially methylated region (DMR) reported previously as the model, a set of primers with different melting temperatures of products in the two sides of MVP were designed. By using the FastDigest methylation-sensitive restriction enzyme (MSRE), DNA digestion, multiplex amplification, MCA detection and MCA profiles were performed in a single reaction tube. Same samples (peripheral venous blood, semen, and vaginal fluid, 5 samples each type) were tested by single-tube one step MVP and traditional MSRE-PCR MCA technology. To verify the feasibility of this method, the results were compared with that of the traditional technology. The MCA/HRM profiles of different samples were analyzed and compared. When the melting temperature of the fragments had a differential of 2 degrees C, the MCA melting peaks separated well, and MCA detection after multiplex amplification was successful. The single-tube PDP-MCA assay was developed, which integrated multiple reactions (digestion, amplification and detection) into one tube. By this method, the sample-specific profiles and data were analyzed in 2 h, which is similar to that of the traditional method. The rapid classifications of the samples were also realized. Multiplex MVPs can be analyzed in a single closed-tube. The single-tube PDP-MCA technology is a simple, fast, and automatable method. It can be used for detection of DNA methylation variations.

  18. Improving single-variable and multivariable techniques for estimating missing hydrological data

    Science.gov (United States)

    Bennis, S.; Berrada, F.; Kang, N.

    1997-04-01

    A highly efficient technique is developed to obtain the best least-squares approximation of the missing hydrological data in the single-variable case, and this is presented here. The technique is based on an appropriate weighing of the estimated values generated by two autoregressive processes operating, respectively, in the forward and backward directions of time. For the multivariable case, the originally of the work presented here consists in the use of the linear regression model with variable coefficients to estimate missing data. As for the single-variable case, two multivariable regression models are calibrated recursively on available data preceding and following the period of missing data. The use of Kalman filter (KF) has improved the accuracy in the estimation of the first missing data including the peak flow. For subsequent missing data the confidence of the estimates is greater when using a static model identified by the ordinary least squares (OLS) technique. It has been found that there is a critical rank for which there is an inversion of performance between the KF and OLS technique. When the period of missing data is smaller than the critical rank we use only KF technique. When the period of missing data extends past the critical rank, it is recommended that KF be used to estimate the first missing data and then use OLS technique to estimate data coming after the critical rank.

  19. Energy cost of walking: solving the paradox of steady state in the presence of variable walking speed.

    Science.gov (United States)

    Plasschaert, Frank; Jones, Kim; Forward, Malcolm

    2009-02-01

    Measurement of the energy cost of walking in children with cerebral palsy is used for baseline and outcome assessment. However, such testing relies on the establishment of steady state that is deemed present when oxygen consumption is stable. This is often assumed when walking speed is constant but in practice, speed can and does vary naturally. Whilst constant speed is achievable on a treadmill, this is often impractical clinically, thus rendering an energy cost test to an element of subjectivity. This paper attempts to address this issue by presenting a new method for calculating energy cost of walking that automatically applies a mathematically defined threshold for steady state within a (non-treadmill) walking trial and then strips out all of the non-steady state events within that trial. The method is compared with a generic approach that does not remove non-steady state data but rather uses an average value over a complete walking trial as is often used in the clinical environment. Both methods were applied to the calculation of several energy cost of walking parameters of self-selected walking speed in a cohort of unimpaired subjects and children with cerebral palsy. The results revealed that both methods were strongly correlated for each parameter but showed systematic significant differences. It is suggested that these differences are introduced by the rejection of non-steady state data that would otherwise have incorrectly been incorporated into the calculation of the energy cost of walking indices during self-selected walking with its inherent speed variation.

  20. Genetic analysis of glucosinolate variability in broccoli florets using genome-anchored single nucleotide polymorphisms.

    Science.gov (United States)

    Brown, Allan F; Yousef, Gad G; Reid, Robert W; Chebrolu, Kranthi K; Thomas, Aswathy; Krueger, Christopher; Jeffery, Elizabeth; Jackson, Eric; Juvik, John A

    2015-07-01

    The identification of genetic factors influencing the accumulation of individual glucosinolates in broccoli florets provides novel insight into the regulation of glucosinolate levels in Brassica vegetables and will accelerate the development of vegetables with glucosinolate profiles tailored to promote human health. Quantitative trait loci analysis of glucosinolate (GSL) variability was conducted with a B. oleracea (broccoli) mapping population, saturated with single nucleotide polymorphism markers from a high-density array designed for rapeseed (Brassica napus). In 4 years of analysis, 14 QTLs were associated with the accumulation of aliphatic, indolic, or aromatic GSLs in floret tissue. The accumulation of 3-carbon aliphatic GSLs (2-propenyl and 3-methylsulfinylpropyl) was primarily associated with a single QTL on C05, but common regulation of 4-carbon aliphatic GSLs was not observed. A single locus on C09, associated with up to 40 % of the phenotypic variability of 2-hydroxy-3-butenyl GSL over multiple years, was not associated with the variability of precursor compounds. Similarly, QTLs on C02, C04, and C09 were associated with 4-methylsulfinylbutyl GSL concentration over multiple years but were not significantly associated with downstream compounds. Genome-specific SNP markers were used to identify candidate genes that co-localized to marker intervals and previously sequenced Brassica oleracea BAC clones containing known GSL genes (GSL-ALK, GSL-PRO, and GSL-ELONG) were aligned to the genomic sequence, providing support that at least three of our 14 QTLs likely correspond to previously identified GSL loci. The results demonstrate that previously identified loci do not fully explain GSL variation in broccoli. The identification of additional genetic factors influencing the accumulation of GSL in broccoli florets provides novel insight into the regulation of GSL levels in Brassicaceae and will accelerate development of vegetables with modified or enhanced GSL

  1. Third-Body Perturbation Using a Single Averaged Model: Application in Nonsingular Variables

    Directory of Open Access Journals (Sweden)

    Carlos Renato Huaura Solórzano

    2007-01-01

    Full Text Available The Lagrange's planetary equations written in terms of the classical orbital elements have the disadvantage of singularities in eccentricity and inclination. These singularities are due to the mathematical model used and do not have physical reasons. In this paper, we studied the third-body perturbation using a single averaged model in nonsingular variables. The goal is to develop a semianalytical study of the perturbation caused in a spacecraft by a third body using a single averaged model to eliminate short-period terms caused by the motion of the spacecraft. This is valid if no resonance occurs with the moon or the sun. Several plots show the time histories of the Keplerian elements of equatorial and circular orbits, which are the situations with singularities. In this paper, the expansions are limited only to second order in eccentricity and for the ratio of the semimajor axis of the perturbing and perturbed bodies and to the fourth order for the inclination.

  2. In-line single-mode fiber variable optical attenuator based on electrically addressable microdroplets

    Science.gov (United States)

    Duduś, A.; Blue, R.; Zagnoni, M.; Stewart, G.; Uttamchandani, D.

    2014-07-01

    We report an in-line, fiber optic, broadband variable optical attenuator employing a side-polished, single-mode optical fiber integrated on a digital microfluidics platform. The system is designed to electrically translate a liquid droplet along the polished surface of an optical fiber using electrowetting forces. This fiber optic device has the advantage of no moving mechanical parts and lends itself to miniaturization. A maximum attenuation of 25 dB has been obtained in the wavelength range between 1520 nm and 1560 nm.

  3. Generalized local test for local extrema in single-variable functions

    Science.gov (United States)

    Gkioulekas, Eleftherios

    2014-01-01

    We give a detailed derivation of a generalization of the second derivative test of single-variable calculus which can classify critical points as local minima or local maxima (or neither), whenever the traditional second derivative test fails, by considering the values of higher-order derivatives evaluated at the critical points. The enhanced test is local, in the sense that it is only necessary to evaluate all relevant derivatives at the critical point itself, and it is reasonably robust. We illustrate an application of the generalized test on a trigonometric function where the second derivative test fails to classify some of the critical points.

  4. Enumeration of Combinatorial Classes of Single Variable Complex Polynomial Vector Fields

    DEFF Research Database (Denmark)

    Dias, Kealey

    in a valid way. We first enumerate all combinatorial classes with respect to degree d, and then we enumerate the combinatorial classes having a specific dimension q in parameter space. In both cases, a recursion equation and implicit expressions for the algebraic generating functions are calculated......A vector field in the space of degree d monic, centered single variable complex polynomial vector fields has a combinatorial structure which can be fully described by a combinatorial data set consisting of an equivalence relation and a marked subset on the integers mod 2d-2, satisfying certain...

  5. Single cell adhesion strength assessed with variable-angle total internal reflection fluorescence microscopy

    Directory of Open Access Journals (Sweden)

    Marcelina Cardoso Dos Santos

    2017-06-01

    Full Text Available We propose a new strategy to evaluate adhesion strength at the single cell level. This approach involves variable-angle total internal reflection fluorescence microscopy to monitor in real time the topography of cell membranes, i.e. a map of the membrane/substrate separation distance. According to the Boltzmann distribution, both potential energy profile and dissociation energy related to the interactions between the cell membrane and the substrate were determined from the membrane topography. We have highlighted on glass substrates coated with poly-L-lysine and fibronectin, that the dissociation energy is a reliable parameter to quantify the adhesion strength of MDA-MB-231 motile cells.

  6. Speed limit of frozen pellets (H{sub 2}, D{sub 2}, and Ne) through single-loop and multiloop tubes and implications for fusion plasma research

    Energy Technology Data Exchange (ETDEWEB)

    Combs, S. K.; Griffith, A. E.; Foust, C. R.

    2001-01-01

    Frozen pellets (H{sub 2}, D{sub 2}, and Ne at 8 K) of nominal 2.7 mm diam were shot through a coiled tube (single loop of {approx}0.6 m diam and 8.5 mm bore), and the speed limit for survival was recorded for each pellet type. Intact H{sub 2} pellets were observed at speeds approaching 500 m/s; but neon pellets could not survive much more than 100 m/s. The speed limit for D{sub 2} pellets fell in the middle at {approx}300 m/s. Some D{sub 2} pellets were also shot through a 30 m coiled tube consisting of 11 loops (average loop diameter of {approx}0.8 m), and a speed limit of {approx}100 m/s was observed. Injection of frozen H{sub 2} or D{sub 2} pellets is commonly used for core fueling of magnetically confined plasmas, and frozen neon pellets are sometimes used for impurity transport studies in similar experiments. The results from these tests add to a pellet database for injection lines with single- and complex multiple-curved guide tubes. All of the information to date suggests that frozen pellets can be delivered reliably from a pellet source to any accessible plasma location on a fusion device via ''roller-coaster'' tubes as long as the pellet speed is maintained below a threshold limit.

  7. The effects of movement speed on kinematic variability and dynamic stability of the trunk in healthy individuals and low back pain patients.

    Science.gov (United States)

    Asgari, Morteza; Sanjari, Mohammad Ali; Mokhtarinia, Hamid Reza; Moeini Sedeh, Samaneh; Khalaf, Kinda; Parnianpour, Mohamad

    2015-08-01

    Comparison of the kinematic variability and dynamic stability of the trunk between healthy and low back pain patient groups can contribute to gaining valuable information about the movement patterns and neuromotor strategies involved in various movement tasks. Fourteen chronic low back pain patients with mild symptoms and twelve healthy male volunteers performed repeated trunk flexion-extension movements in the sagittal plane at three different speeds: 20 cycles/min, self-selected, and 40 cycles/min. Mean standard deviations, coefficient of variation and variance ratio as variability measures; maximum finite-time Lyapunov exponents and maximum Floquet multipliers as stability measures were computed from trunk kinematics. Higher speed significantly reduced the kinematic variability, while it increased short-term Lyapunov exponents. Long-term Lyapunov exponents were higher at self-selected speed and lower in low back pain patients as compared to control volunteers. Floquet multipliers were larger at self-selected speed and during higher pace trunk movements. Our findings suggest that slower pace flexion-extension trunk movements are associated with more motor variation as well as local and orbital stability, implying less potential risk of injury for the trunk. Individuals with and without low back pain consistently recruited a closed-loop control strategy towards achieving trunk stability. Chronic low back pain patients exhibited more stable trunk movements over long-term periods, indicating probable temporary pain relief functional adaption strategies. These results may be used towards the development of more effective personalized rehabilitation strategies and quantitative spinal analysis tools for low back pain detection, diagnosis and treatment, as well as improvement of workspace and occupational settings. Copyright © 2015 Elsevier Ltd. All rights reserved.

  8. DAC to Mitigate the Effect of Periodic Disturbances on Drive Train using Collective Pitch for Variable Speed Wind Turbine

    DEFF Research Database (Denmark)

    Imran, Raja Muhammad; Hussain, Dil Muhammad Akbar; Soltani, Mohsen

    2015-01-01

    scheme to mitigate the effect of 3p flicker on drive train. 5MW wind turbine of the National Renewable Laboratories (NREL) is used as research object and results are simulated in MATLAB/Simulink. We designed the controller based on linearized model of the wind turbine generated for above rated wind speed......DAC is a linear control technique used to mitigate the effect of disturbance on the plant. It is a superposition of full state feedback and disturbance feedback. This paper presents a control technique based on Disturbance Accommodation Control (DAC) to reduce fatigue on drive train generated...... by wind which is combination of step and periodic disturbances (generated by wind shear and tower shadow ) of the turbine for above rated wind speed (Region III). We have presented a wind modeling for wind shear and tower shadow effect for the wind turbine control simulation and also developed a control...

  9. Single-end-access distributed strain sensing with wide dynamic range using higher-speed Brillouin optical correlation-domain reflectometry

    Science.gov (United States)

    Mizuno, Yosuke; Hayashi, Neisei; Fukuda, Hideyuki; Nakamura, Kentaro

    2017-07-01

    Single-end-access real-time fiber-optic distributed strain sensing has recently been demonstrated using an ultrahigh-speed configuration of Brillouin optical correlation-domain reflectometry (BOCDR). Its extremely high sampling rate was, however, achieved at the cost of a limited strain dynamic range (cost-effective higher-speed configuration of BOCDR with a wide strain dynamic range (up to 2.0%; evaluated by static strain measurement). This value is larger than that of any other BOCDR configuration. Using this configuration, we demonstrate some fundamental distributed strain measurements and breakage detection.

  10. Relationship of Speed, Agility, Neuromuscular Power, and Selected Anthropometrical Variables and Performance Results of Male and Female Junior Tennis Players

    OpenAIRE

    Munivrana, Goran; Filipčić, Aleš; Filipčić, Tjaša

    2015-01-01

    The aim of the study was to analyses the relation between the selected speed, agility, and neuromuscular power test items. The sample of subjects consisted of 154 male and 152 female young tennis players. Using six motor and three anthropometrical tests we investigate differences between males and females and between two age categories. Finally, we analyzed the relation between motor and anthropometrical tests and a player’s tennis performance. The correlation between the two agil...

  11. Design optimization under uncertainty and speed variability for a piezoelectric energy harvester powering a tire pressure monitoring sensor

    Science.gov (United States)

    Toghi Eshghi, Amin; Lee, Soobum; Kazem Sadoughi, Mohammad; Hu, Chao; Kim, Young-Cheol; Seo, Jong-Ho

    2017-10-01

    Energy harvesting (EH) technologies to power small sized electronic devices are attracting great attention. Wasted energy in a vehicle’s rotating tire has a great potential to enable self-powered tire pressure monitoring sensors (TPMS). Piezoelectric type energy harvesters can be used to collect vibrational energy and power such systems. Due to the presence of harsh acceleration in a rotating tire, a design tradeoff needs to be studied to prolong the harvester’s fatigue life as well as to ensure sufficient power generation. However, the design by traditional deterministic design optimization (DDO) does not show reliable performance due to the lack of consideration of various uncertainty factors (e.g., manufacturing tolerances, material properties, and loading conditions). In this study, we address a new EH design formulation that considers the uncertainty in car speed, dimensional tolerances and material properties, and solve this design problem using reliability-based design optimization (RBDO). The RBDO problem is formulated to maximize compactness and minimize weight of a TPMS harvester while satisfying power and durability requirements. A transient analysis has been done to measure the time varying response of EH such as power generation, dynamic strain, and stress. A conservative design formulation is proposed to consider the expected power from varied speed and stress at higher speed. When compared to the DDO, the RBDO results show that the reliability of EH is increased significantly by scarifying the objective function. Finally, experimental test has been conducted to demonstrate the merits of RBDO design over DDO.

  12. High performance SPWM frequency converter three-phase cage induction motor's synchronous modulation variable frequency speed regulation system

    Energy Technology Data Exchange (ETDEWEB)

    Zhang Xiaomei; Chen Yaozhong [Taiyuan University of Technology, College of Information Engineering, Shanxi (China)

    2000-08-01

    The paper discusses the synchronous modulation model of SPWM frequency converter at the carrier ratio N=27, and presents the interval values of a small period pulse at modulation depth M=0.1{approx} 0.7 and the line-voltage u{sub AB(t)} express formula of double-pole modulation at M=0.1. Basing on the parameters of a practical three-phase cage induction motor the fundamental frequency f{sub 1} and mechanical characteristic parameters are calculated. The system's control part is simple, the mechanical characteristic is hard and running steadily at a low speed. So it can constitute high performance system with variable frequency and speed regulation. (orig.)

  13. Randomised clinical trial on the effect of a single oral administration of l-tryptophan, at three dose rates, on reaction speed, plasma concentration and haemolysis in horses.

    Science.gov (United States)

    Noble, Glenys K; Li, Xiuhua; Zhang, Dagong; Sillence, Martin N

    2016-07-01

    Tryptophan (TRP) is marketed as a calmative for horses despite reservations about its efficacy. The aim of this study was to measure the effect of oral TRP administration on the reaction speed of horses. Sixty mature horses were used in a two stage randomised, blind, cross-over study, receiving a placebo and an oral dose of TRP (30, 60 or 120 mg/kg body weight), before undergoing a reaction speed test. Blood samples were taken up to 96 h after TRP administration, to identify signs of acute haemolytic anaemia. Plasma TRP concentrations were increased (P reaction speed of horses when startled. There was no evidence of alterations in clinical pathology parameters in 432 blood samples. While the safety of these doses of TRP can be confirmed, there was no evidence to suggest that a single dose of TRP is an effective calmative for horses. Copyright © 2016 Elsevier Ltd. All rights reserved.

  14. THE EFFECTS OF SINGLE LEG HOP PROGRESSION AND DOUBLE LEGS HOP PROGRESSION EXERCISE TO INCREASE SPEED AND EXPLOSIVE POWER OF LEG MUSCLE

    Directory of Open Access Journals (Sweden)

    Nining W. Kusnanik

    2015-05-01

    Full Text Available The main purpose of this study was to determine the effect of single leg hop progression and double legs hop progression exercise to increase speed and explosive power of leg muscles. Plyometric is one of the training methods that can increase explosive power. There are many models of plyometric training including single leg hop progression and double leg hop progression. This research was experimental using match subject design techniques. The subjects of this study were 39 students who joined basketball school club. There were 3 groups in this study: Group 1 were 13 students who given sin¬gle leg hop progression exercise, Group 2 were 13 students who given double legs hop progression exercise, Group 3 were 13 students who given conventional exercise. The data was collected during pre test and post test by testing 30m speed running and vertical jump. The data was analyzed using Analysis of Varians (Anova. It was found that there were significantly increased on speed and explosive power of leg muscles of Group 1 and Group 2. It can be stated that single leg hop progression exercise was more effective than double leg hop progression exercise. The recent findings supported the hypothesis that single leg hop progression and double legs hop progression exercise can increase speed and explosive power of leg muscles. These finding were supported by some previous studies (Singh, et al, 2011; Shallaby, H.K., 2010. The single leg hop progression is more effective than double legs hop progression. This finding was consistent with some previous evidences (McCurdy, et al, 2005; Makaruk et al, 2011.

  15. Variable-Speed Wind Turbine Controller Systematic Design Methodology: A Comparison of Non-Linear and Linear Model-Based Designs

    Energy Technology Data Exchange (ETDEWEB)

    Hand, M. M.

    1999-07-30

    Variable-speed, horizontal axis wind turbines use blade-pitch control to meet specified objectives for three regions of operation. This paper focuses on controller design for the constant power production regime. A simple, rigid, non-linear turbine model was used to systematically perform trade-off studies between two performance metrics. Minimization of both the deviation of the rotor speed from the desired speed and the motion of the actuator is desired. The robust nature of the proportional-integral-derivative (PID) controller is illustrated, and optimal operating conditions are determined. Because numerous simulation runs may be completed in a short time, the relationship of the two opposing metrics is easily visualized. Traditional controller design generally consists of linearizing a model about an operating point. This step was taken for two different operating points, and the systematic design approach was used. A comparison of the optimal regions selected using the n on-linear model and the two linear models shows similarities. The linearization point selection does, however, affect the turbine performance slightly. Exploitation of the simplicity of the model allows surfaces consisting of operation under a wide range of gain values to be created. This methodology provides a means of visually observing turbine performance based upon the two metrics chosen for this study. Design of a PID controller is simplified, and it is possible to ascertain the best possible combination of controller parameters. The wide, flat surfaces indicate that a PID controller is very robust in this variable-speed wind turbine application.

  16. Older adults must hurry at pedestrian lights! A cross-sectional analysis of preferred and fast walking speed under single- and dual-task conditions.

    Directory of Open Access Journals (Sweden)

    Patrick Eggenberger

    Full Text Available Slow walking speed is strongly associated with adverse health outcomes, including cognitive impairment, in the older population. Moreover, adequate walking speed is crucial to maintain older pedestrians' mobility and safety in urban areas. This study aimed to identify the proportion of Swiss older adults that didn't reach 1.2 m/s, which reflects the requirements to cross streets within the green-yellow phase of pedestrian lights, when walking fast under cognitive challenge. A convenience sample, including 120 older women (65% and men, was recruited from the community (88% and from senior residences and divided into groups of 70-79 years (n = 59, 74.8 ± 0.4 y; mean ± SD and ≥80 years (n = 61, 85.5 ± 0.5 y. Steady state walking speed was assessed under single- and dual-task conditions at preferred and fast walking speed. Additionally, functional lower extremity strength (5-chair-rises test, subjective health rating, and retrospective estimates of fall frequency were recorded. Results showed that 35.6% of the younger and 73.8% of the older participants were not able to walk faster than 1.2 m/s under the fast dual-task walking condition. Fast dual-task walking speed was higher compared to the preferred speed single- and dual-task conditions (all p < .05, r = .31 to .48. Average preferred single-task walking speed was 1.19 ± 0.24 m/s (70-79 y and 0.94 ± 0.27 m/s (≥80 y, respectively, and correlated with performance in the 5-chair-rises test (rs = -.49, p < .001, subjective health (τ = .27, p < .001, and fall frequency (τ = -.23, p = .002. We conclude that the fitness status of many older people is inadequate to safely cross streets at pedestrian lights and maintain mobility in the community's daily life in urban areas. Consequently, training measures to improve the older population's cognitive and physical fitness should be promoted to enhance walking speed and safety of older pedestrians.

  17. Coping with Harmonics in Smart Grid: variable speed driveswith Back-to-Back Voltage Source Converter versus the matrix converter

    OpenAIRE

    Ali, Imran

    2013-01-01

    In this master thesis it has been investigated to utilize the matrix as a shunt active power filter and an adjustable speed drive and then compare the matrix converter with the Back-to-Back voltage source converter in terms of active filtering capability and reactive power compensation.In first steps to utilize the matrix converter as a motor drive and a shunt active power filter the control systems for the Permanent magnet synchronous machine, shunt active power filter and 3-phase LC-filter ...

  18. Self-Calibrating Wave-Encoded Variable-Density Single-Shot Fast Spin Echo Imaging.

    Science.gov (United States)

    Chen, Feiyu; Taviani, Valentina; Tamir, Jonathan I; Cheng, Joseph Y; Zhang, Tao; Song, Qiong; Hargreaves, Brian A; Pauly, John M; Vasanawala, Shreyas S

    2017-09-14

    It is highly desirable in clinical abdominal MR scans to accelerate single-shot fast spin echo (SSFSE) imaging and reduce blurring due to T2 decay and partial-Fourier acquisition. To develop and investigate the clinical feasibility of wave-encoded variable-density SSFSE imaging for improved image quality and scan time reduction. Prospective controlled clinical trial. With Institutional Review Board approval and informed consent, the proposed method was assessed on 20 consecutive adult patients (10 male, 10 female, range, 24-84 years). A wave-encoded variable-density SSFSE sequence was developed for clinical 3.0T abdominal scans to enable high acceleration (3.5×) with full-Fourier acquisitions by: 1) introducing wave encoding with self-refocusing gradient waveforms to improve acquisition efficiency; 2) developing self-calibrated estimation of wave-encoding point-spread function and coil sensitivity to improve motion robustness; and 3) incorporating a parallel imaging and compressed sensing reconstruction to reconstruct highly accelerated datasets. Image quality was compared pairwise with standard Cartesian acquisition independently and blindly by two radiologists on a scale from -2 to 2 for noise, contrast, confidence, sharpness, and artifacts. The average ratio of scan time between these two approaches was also compared. A Wilcoxon signed-rank tests with a P value under 0.05 considered statistically significant. Wave-encoded variable-density SSFSE significantly reduced the perceived noise level and improved the sharpness of the abdominal wall and the kidneys compared with standard acquisition (mean scores 0.8, 1.2, and 0.8, respectively, P variable-density sampling SSFSE achieves improved image quality with clinically relevant echo time and reduced scan time, thus providing a fast and robust approach for clinical SSFSE imaging. 1 TECHNICAL EFFICACY: Stage 6 J. Magn. Reson. Imaging 2017. © 2017 International Society for Magnetic Resonance in Medicine.

  19. Power quality control and design of power converter for variable-speed wind energy conversion system with permanent-magnet synchronous generator.

    Science.gov (United States)

    Oğuz, Yüksel; Güney, İrfan; Çalık, Hüseyin

    2013-01-01

    The control strategy and design of an AC/DC/AC IGBT-PMW power converter for PMSG-based variable-speed wind energy conversion systems (VSWECS) operation in grid/load-connected mode are presented. VSWECS consists of a PMSG connected to a AC-DC IGBT-based PWM rectifier and a DC/AC IGBT-based PWM inverter with LCL filter. In VSWECS, AC/DC/AC power converter is employed to convert the variable frequency variable speed generator output to the fixed frequency fixed voltage grid. The DC/AC power conversion has been managed out using adaptive neurofuzzy controlled inverter located at the output of controlled AC/DC IGBT-based PWM rectifier. In this study, the dynamic performance and power quality of the proposed power converter connected to the grid/load by output LCL filter is focused on. Dynamic modeling and control of the VSWECS with the proposed power converter is performed by using MATLAB/Simulink. Simulation results show that the output voltage, power, and frequency of VSWECS reach to desirable operation values in a very short time. In addition, when PMSG based VSWECS works continuously with the 4.5 kHz switching frequency, the THD rate of voltage in the load terminal is 0.00672%.

  20. Power Quality Control and Design of Power Converter for Variable-Speed Wind Energy Conversion System with Permanent-Magnet Synchronous Generator

    Science.gov (United States)

    Oğuz, Yüksel; Güney, İrfan; Çalık, Hüseyin

    2013-01-01

    The control strategy and design of an AC/DC/AC IGBT-PMW power converter for PMSG-based variable-speed wind energy conversion systems (VSWECS) operation in grid/load-connected mode are presented. VSWECS consists of a PMSG connected to a AC-DC IGBT-based PWM rectifier and a DC/AC IGBT-based PWM inverter with LCL filter. In VSWECS, AC/DC/AC power converter is employed to convert the variable frequency variable speed generator output to the fixed frequency fixed voltage grid. The DC/AC power conversion has been managed out using adaptive neurofuzzy controlled inverter located at the output of controlled AC/DC IGBT-based PWM rectifier. In this study, the dynamic performance and power quality of the proposed power converter connected to the grid/load by output LCL filter is focused on. Dynamic modeling and control of the VSWECS with the proposed power converter is performed by using MATLAB/Simulink. Simulation results show that the output voltage, power, and frequency of VSWECS reach to desirable operation values in a very short time. In addition, when PMSG based VSWECS works continuously with the 4.5 kHz switching frequency, the THD rate of voltage in the load terminal is 0.00672%. PMID:24453905

  1. Aerodynamic Effects of High Turbulence Intensity on a Variable-Speed Power-Turbine Blade With Large Incidence and Reynolds Number Variations

    Science.gov (United States)

    Flegel, Ashlie B.; Giel, Paul W.; Welch, Gerard E.

    2014-01-01

    The effects of high inlet turbulence intensity on the aerodynamic performance of a variable speed power turbine blade are examined over large incidence and Reynolds number ranges. These results are compared to previous measurements made in a low turbulence environment. Both high and low turbulence studies were conducted in the NASA Glenn Research Center Transonic Turbine Blade Cascade Facility. The purpose of the low inlet turbulence study was to examine the transitional flow effects that are anticipated at cruise Reynolds numbers. The current study extends this to LPT-relevant turbulence levels while perhaps sacrificing transitional flow effects. Assessing the effects of turbulence at these large incidence and Reynolds number variations complements the existing database. Downstream total pressure and exit angle data were acquired for 10 incidence angles ranging from +15.8deg to -51.0deg. For each incidence angle, data were obtained at five flow conditions with the exit Reynolds number ranging from 2.12×10(exp 5) to 2.12×10(exp 6) and at a design exit Mach number of 0.72. In order to achieve the lowest Reynolds number, the exit Mach number was reduced to 0.35 due to facility constraints. The inlet turbulence intensity, Tu, was measured using a single-wire hotwire located 0.415 axial-chord upstream of the blade row. The inlet turbulence levels ranged from 8 to 15 percent for the current study. Tu measurements were also made farther upstream so that turbulence decay rates could be calculated as needed for computational inlet boundary conditions. Downstream flow field measurements were obtained using a pneumatic five-hole pitch/yaw probe located in a survey plane 7 percent axial chord aft of the blade trailing edge and covering three blade passages. Blade and endwall static pressures were acquired for each flow condition as well. The blade loading data show that the suction surface separation that was evident at many of the low Tu conditions has been eliminated. At

  2. Aerodynamic Effects of Turbulence Intensity on a Variable-Speed Power-Turbine Blade with Large Incidence and Reynolds Number Variations

    Science.gov (United States)

    Flegel, Ashlie Brynn; Giel, Paul W.; Welch, Gerard E.

    2014-01-01

    The effects of inlet turbulence intensity on the aerodynamic performance of a variable speed power turbine blade are examined over large incidence and Reynolds number ranges. Both high and low turbulence studies were conducted in the NASA Glenn Research Center Transonic Turbine Blade Cascade Facility. The purpose of the low inlet turbulence study was to examine the transitional flow effects that are anticipated at cruise Reynolds numbers. The high turbulence study extends this to LPT-relevant turbulence levels while perhaps sacrificing transitional flow effects. Downstream total pressure and exit angle data were acquired for ten incidence angles ranging from +15.8 to 51.0. For each incidence angle, data were obtained at five flow conditions with the exit Reynolds number ranging from 2.12105 to 2.12106 and at a design exit Mach number of 0.72. In order to achieve the lowest Reynolds number, the exit Mach number was reduced to 0.35 due to facility constraints. The inlet turbulence intensity, Tu, was measured using a single-wire hotwire located 0.415 axial-chord upstream of the blade row. The inlet turbulence levels ranged from 0.25 - 0.4 for the low Tu tests and 8- 15 for the high Tu study. Tu measurements were also made farther upstream so that turbulence decay rates could be calculated as needed for computational inlet boundary conditions. Downstream flow field measurements were obtained using a pneumatic five-hole pitchyaw probe located in a survey plane 7 axial chord aft of the blade trailing edge and covering three blade passages. Blade and endwall static pressures were acquired for each flow condition as well. The blade loading data show that the suction surface separation that was evident at many of the low Tu conditions has been eliminated. At the extreme positive and negative incidence angles, the data show substantial differences in the exit flow field. These differences are attributable to both the higher inlet Tu directly and to the thinner inlet endwall

  3. Natural Language Search Interfaces: Health Data Needs Single-Field Variable Search

    Science.gov (United States)

    Smith, Sam; Sufi, Shoaib; Goble, Carole; Buchan, Iain

    2016-01-01

    Background Data discovery, particularly the discovery of key variables and their inter-relationships, is key to secondary data analysis, and in-turn, the evolving field of data science. Interface designers have presumed that their users are domain experts, and so they have provided complex interfaces to support these “experts.” Such interfaces hark back to a time when searches needed to be accurate first time as there was a high computational cost associated with each search. Our work is part of a governmental research initiative between the medical and social research funding bodies to improve the use of social data in medical research. Objective The cross-disciplinary nature of data science can make no assumptions regarding the domain expertise of a particular scientist, whose interests may intersect multiple domains. Here we consider the common requirement for scientists to seek archived data for secondary analysis. This has more in common with search needs of the “Google generation” than with their single-domain, single-tool forebears. Our study compares a Google-like interface with traditional ways of searching for noncomplex health data in a data archive. Methods Two user interfaces are evaluated for the same set of tasks in extracting data from surveys stored in the UK Data Archive (UKDA). One interface, Web search, is “Google-like,” enabling users to browse, search for, and view metadata about study variables, whereas the other, traditional search, has standard multioption user interface. Results Using a comprehensive set of tasks with 20 volunteers, we found that the Web search interface met data discovery needs and expectations better than the traditional search. A task × interface repeated measures analysis showed a main effect indicating that answers found through the Web search interface were more likely to be correct (F 1,19=37.3, Psearch interface (F 1,19=18.0, Psearch interface received significantly higher ratings than the traditional

  4. Natural Language Search Interfaces: Health Data Needs Single-Field Variable Search.

    Science.gov (United States)

    Jay, Caroline; Harper, Simon; Dunlop, Ian; Smith, Sam; Sufi, Shoaib; Goble, Carole; Buchan, Iain

    2016-01-14

    Data discovery, particularly the discovery of key variables and their inter-relationships, is key to secondary data analysis, and in-turn, the evolving field of data science. Interface designers have presumed that their users are domain experts, and so they have provided complex interfaces to support these "experts." Such interfaces hark back to a time when searches needed to be accurate first time as there was a high computational cost associated with each search. Our work is part of a governmental research initiative between the medical and social research funding bodies to improve the use of social data in medical research. The cross-disciplinary nature of data science can make no assumptions regarding the domain expertise of a particular scientist, whose interests may intersect multiple domains. Here we consider the common requirement for scientists to seek archived data for secondary analysis. This has more in common with search needs of the "Google generation" than with their single-domain, single-tool forebears. Our study compares a Google-like interface with traditional ways of searching for noncomplex health data in a data archive. Two user interfaces are evaluated for the same set of tasks in extracting data from surveys stored in the UK Data Archive (UKDA). One interface, Web search, is "Google-like," enabling users to browse, search for, and view metadata about study variables, whereas the other, traditional search, has standard multioption user interface. Using a comprehensive set of tasks with 20 volunteers, we found that the Web search interface met data discovery needs and expectations better than the traditional search. A task × interface repeated measures analysis showed a main effect indicating that answers found through the Web search interface were more likely to be correct (F1,19=37.3, Pserendipity as part of the refinement. The results provide clear evidence that data science should adopt single-field natural language search interfaces for

  5. The Effects of Heart Rate Versus Speed-Based High-Intensity Interval Training on Heart Rate Variability in Young Females

    Directory of Open Access Journals (Sweden)

    Maryam Rabbani

    2017-06-01

    Full Text Available Introduction: The aim of this study was to compare the effects of high-intensity interval training (HIT prescription by heart rate (HR-based and running speed (speed-based methods on natural logarithm of the square root of the mean of the sum of the squares of differences between adjacent normal R-R intervals (Ln rMSSD as a measure of heart rate variability (HRV in young female student athletes. Methods: Seventeen female student athletes participated in this study and were divided into HR-based (n=9, age: 16.7 years and speed-based (n=8, age: 16.9 years HIT groups. 30-15 Intermittent Fitness Test was used for the speed-based group to detect the reference maximum speed (VIFT for prescribing the HIT intensity accordingly. Age predicted maximal HR was used for the HR-based group as the reference value. All subjects performed similar training protocol for 5 weeks, except the method of individualizing HIT sessions (2 weekly sessions of HIT=3 sets of 3 minutes work interspersed with 3 minutes passive recovery with the 15-15 seconds format during each working set; either according to 90%-95% of maximal HR or VIFT. Results: HR- and speed-based HIT groups showed the most likely large improvements in Ln rMSSD of +7.9%, 90% confidence limits [CL] (5.9; 10.0; standardized change: +1.75 (1.32; 2.19 and +5.5%, (2.8; 8.3; +1.41 (0.72; 2.09, respectively. In between group analyses, HR-based HIT produced likely a small greater improvement in Ln rMSSD than speed-based HIT (+1.9%, [-5.0; 4.4]; +0.50 [-0.14; 1.14], chances for greater/similar/lower values of 79/17/4. Conclusion: It is concluded that both HIT prescription strategies were effective in Ln rMSSD elevation, but using maximal HR as a reference may elicit higher parasympathetic dominance with small effect in young female student athletes.

  6. Variable Light Speed Cosmology(VSL) for the Kalbermann-Halevi Ansatz Under The Concept Of The Macroscopic Spacetime Shortcuts In The Manyfold Universe

    CERN Document Server

    Loup, F

    2004-01-01

    We present the Manyfold Universe as the unique scientific explanation for the Mistery of Dark Matter.We present the fact that the exotic particles such as Photinos,Axions,WIMPS,Neutralinos etc all created to explain the Mistery of Dark Matter dont have physical existance because these were not found by a precise instrument as the NASA Chandra Satellite and NASA seems to agree with the Manyfold Universe. Also we study the consequences of this model and the connections between the Manyfold Universe and Variable Light Speed Cosmology.

  7. Retargeted oncolytic adenovirus displaying a single variable domain of camelid heavy-chain-only antibody in a fiber protein

    Directory of Open Access Journals (Sweden)

    Elisabeth A van Erp

    2015-01-01

    Full Text Available Conditionally replicative adenoviruses are promising agents for oncolytic virotherapy. Various approaches have been attempted to retarget adenoviruses to tumor-specific antigens to circumvent deficiency of receptor for adenoviral binding and to provide an additional level of tumor specificity. Functional incorporation of highly specific targeting molecules into the viral capsid can potentially retarget adenoviral infection. However, conventional antibodies are not compatible with the cytoplasmic adenovirus capsid synthesis. The goal of this study was to evaluate the utility of single variable domains derived from heavy chain camelid antibodies for retargeting of adenovirus infection. We have combined transcriptional targeting using a tumor-specific promoter with transductional targeting through viral capsid incorporation of antihuman carcinoembryonic antigen single variable domains. Obtained data demonstrated that employment of a single variable domain genetically incorporated into an adenovirus fiber increased specificity of infection and efficacy of replication of single variable domain-targeted oncolytic adenovirus. The double targeting, both transcriptional through the C-X-C chemokine receptor type 4 promoter and transductional using the single variable domain, is a promising means to improve the therapeutic index for these advanced generation conditionally replicative adenoviruses. A successful strategy to transductional retargeting of oncolytic adenovirus infection has not been shown before and therefore we believe this is the first employment of transductional targeting using single variable domains derived from heavy chain camelid antibodies to enhance specificity of conditionally replicative adenoviruses.

  8. Single-Machine Group Scheduling Problems with Variable Job Processing Times

    Directory of Open Access Journals (Sweden)

    Ping Ji

    2015-01-01

    Full Text Available This paper considers two resource constrained single-machine group scheduling problems. These problems involve variable job processing times (general position-dependent learning effects and deteriorating jobs; that is, the processing time of a job is defined by the function that involves its starting time and position in the group, and groups’ setup time is a positive strictly decreasing continuous function of the amount of consumed resource. Polynomial time algorithms are proposed to optimally solve the makespan minimization problem under the constraint that the total resource consumption does not exceed a given limit and the total resource consumption minimization problem under the constraint that the makespan does not exceed a given limit, respectively.

  9. Bit-rate-variable and order-switchable optical multiplexing of high-speed pseudorandom bit sequence using optical delays.

    Science.gov (United States)

    Wu, Xiaoxia; Wang, Jian; Yilmaz, Omer F; Nuccio, Scott R; Bogoni, Antonella; Willner, Alan E

    2010-09-15

    We experimentally demonstrate high-speed optical pseudorandom bit sequence (PRBS) multiplexing with coarse and fine bit-rate tuning capability and a switchable order using optical delays. Data multiplexing of 80 Gbit/s and 160 Gbit/s is shown, each with a tunable rate using a conversion/dispersion-based continuously tunable optical delay and tunable PRBS order with large switchable fiber delays. A 7% bit-rate tunability, i.e., 80-85.6 Gbit/s and 160-171.2 Gbit/s, is shown for both 2(7)-1 and 2(15)-1 PRBS. The rf spectra before and after multiplexing are measured in each case and show a suppression ratio of >30 dB, exhibiting the expected PRBS spectral characteristics.

  10. Calibration of ultra high speed laser engraving processes by correlating influencing variables including correlative evaluation with SEM and CLSM

    Science.gov (United States)

    Bohrer, Markus; Vaupel, Matthias; Nirnberger, Robert; Weinberger, Bernhard

    2016-03-01

    Laser engraving is used for decades as a well-established process e. g. for the production of print and embossing forms for many goods in daily life, e. g. decorated cans and printed bank notes. Up to now it is more or less a so-called fire-and-forget process. From the original artist's plan to the digitization, then from the laser source itself (with electronic signals, RF and plasma discharge regarding CO2 lasers) to the behavior of the optical beam delivery — especially if an AOM is used — to the interaction of the laser beam with the material itself is a long process chain. The most recent results using CO2 lasers with AOMs and the research done with scanning electron microscope (SEM) and confocal laser scanning microscope (CLSM) — as a set for correlative microscopy to evaluate the high speed engraving characteristics — are presented in this paper.

  11. Comparison of Sliding Mode Control and Fuzzy Logic control applied to Variable Speed Wind Energy Conversion Systems

    Directory of Open Access Journals (Sweden)

    Souhila Rached Zine

    2015-08-01

    Full Text Available wind energy features prominently as a supplementary energy booster. It does not pollute and is inexhaustible. However, its high cost is a major constraint, especially on the less windy sites. The purpose of wind energy systems is to maximize energy efficiency, and extract maximum power from the wind speed. In This case, the MPPT control becomes important. To realize this control, strategy conventional Proportional and Integral (PI controller is usually used. However, this strategy cannot achieve better performance. This paper proposes other control methods of a turbine which optimizes its production such as fuzzy logic, sliding mode control. These methods improve the quality and energy efficiency. The proposed Sliding Mode Control (SMC strategy and the fuzzy controllers have presented attractive features such as robustness to parametric uncertainties of the turbine, simplicity of its design and good performances. The simulation result under Matlab\\Simulink has validated the performance of the proposed MPPT strategies.

  12. Heart Rate Variability Biofeedback Stress Relief Program for Depression*. A Replicated Single-Subject Design.

    Science.gov (United States)

    Hartogs, Bregje M A; Bartels-Velthuis, Agna A; Van der Ploeg, Karen; Bos, Elske H

    2017-07-19

    Depressive disorders often have a chronic course and the efficacy of evidence-based treatments may be overestimated. To examine the effectiveness of the Heart Rate Variability Stress Reduction Program (SRP) as a supplement to standard treatment in patients with depressive disorders. The SRP was individually administered in eight weekly sessions. Seven participants completed the full protocol and were enrolled in a single-subject ABA multiple baseline experimental design. To perform interrupted time-series analyses, daily measures were completed in a diary (depression, resilience, happiness, heart coherence and a personalized outcome measure). Five out of seven patients improved in depressed mood and/or a personalized outcome measure. The effect of treatment was reversed in four patients during the withdrawal phase. One patient reliably improved on depression, whereas two patients recovered on autonomy and one on social optimism. No consistent relationship was found between the heart rate variability-related level of coherence and self-reported mood levels. The SRP is beneficial in some domains and for some patients. A prolonged treatment or continued home practice may be required for enduring effects. The intervention had more clinical impact on resilience-related outcome measures than on symptoms. The small sample size does not permit generalization of the results. We recommend future investigation of the underlying mechanisms of the SRP.

  13. Variability and Reliability of Single-Walled Carbon Nanotube Field Effect Transistors

    Directory of Open Access Journals (Sweden)

    Ahmad Ehteshamul Islam

    2013-09-01

    Full Text Available Excellent electrical performance and extreme sensitivity to chemical species in semiconducting Single-Walled Carbon NanoTubes (s-SWCNTs motivated the study of using them to replace silicon as a next generation field effect transistor (FET for electronic, optoelectronic, and biological applications. In addition, use of SWCNTs in the recently studied flexible electronics appears more promising because of SWCNTs’ inherent flexibility and superior electrical performance over silicon-based materials. All these applications require SWCNT-FETs to have a wafer-scale uniform and reliable performance over time to a level that is at least comparable with the currently used silicon-based nanoscale FETs. Due to similarity in device configuration and its operation, SWCNT-FET inherits most of the variability and reliability concerns of silicon-based FETs, namely the ones originating from line edge roughness, metal work-function variation, oxide defects, etc. Additional challenges arise from the lack of chirality control in as-grown and post-processed SWCNTs and also from the presence of unstable hydroxyl (–OH groups near the interface of SWCNT and dielectric. In this review article, we discuss these variability and reliability origins in SWCNT-FETs. Proposed solutions for mitigating each of these sources are presented and a future perspective is provided in general, which are required for commercial use of SWCNT-FETs in future nanoelectronic applications.

  14. In-process tool rotational speed variation with constant heat input in friction stir welding of AZ31 sheets with variable thickness

    Science.gov (United States)

    Buffa, Gianluca; Campanella, Davide; Forcellese, Archimede; Fratini, Livan; Simoncini, Michela

    2017-10-01

    In the present work, friction stir welding experiments on AZ31 magnesium alloy sheets, characterized by a variable thickness along the welding line, were carried out. The approach adapted during welding consisted in maintaining constant the heat input to the joint. To this purpose, the rotational speed of the pin tool was increased with decreasing thickness and decreased with increasing thickness in order to obtain the same temperatures during welding. The amount by which the rotational speed was changed as a function of the sheet thickness was defined on the basis of the results given by FEM simulations of the FSW process. Finally, the effect of the in-process variation of the tool rotational speed on the mechanical and microstructural properties of FSWed joints was analysed by comparing both the nominal stress vs. nominal strain curves and microstructure of FSWed joints obtained in different process conditions. It was observed that FSW performed by keeping constant the heat input to the joint leads to almost coincident results both in terms of the curve shape, ultimate tensile strength and ultimate elongation values, and microstructure.

  15. Serial correlation in neural spike trains: experimental evidence, stochastic modeling, and single neuron variability.

    Science.gov (United States)

    Farkhooi, Farzad; Strube-Bloss, Martin F; Nawrot, Martin P

    2009-02-01

    The activity of spiking neurons is frequently described by renewal point process models that assume the statistical independence and identical distribution of the intervals between action potentials. However, the assumption of independent intervals must be questioned for many different types of neurons. We review experimental studies that reported the feature of a negative serial correlation of neighboring intervals, commonly observed in neurons in the sensory periphery as well as in central neurons, notably in the mammalian cortex. In our experiments we observed the same short-lived negative serial dependence of intervals in the spontaneous activity of mushroom body extrinsic neurons in the honeybee. To model serial interval correlations of arbitrary lags, we suggest a family of autoregressive point processes. Its marginal interval distribution is described by the generalized gamma model, which includes as special cases the log-normal and gamma distributions, which have been widely used to characterize regular spiking neurons. In numeric simulations we investigated how serial correlation affects the variance of the neural spike count. We show that the experimentally confirmed negative correlation reduces single-neuron variability, as quantified by the Fano factor, by up to 50%, which favors the transmission of a rate code. We argue that the feature of a negative serial correlation is likely to be common to the class of spike-frequency-adapting neurons and that it might have been largely overlooked in extracellular single-unit recordings due to spike sorting errors.

  16. Serial correlation in neural spike trains: Experimental evidence, stochastic modeling, and single neuron variability

    Science.gov (United States)

    Farkhooi, Farzad; Strube-Bloss, Martin F.; Nawrot, Martin P.

    2009-02-01

    The activity of spiking neurons is frequently described by renewal point process models that assume the statistical independence and identical distribution of the intervals between action potentials. However, the assumption of independent intervals must be questioned for many different types of neurons. We review experimental studies that reported the feature of a negative serial correlation of neighboring intervals, commonly observed in neurons in the sensory periphery as well as in central neurons, notably in the mammalian cortex. In our experiments we observed the same short-lived negative serial dependence of intervals in the spontaneous activity of mushroom body extrinsic neurons in the honeybee. To model serial interval correlations of arbitrary lags, we suggest a family of autoregressive point processes. Its marginal interval distribution is described by the generalized gamma model, which includes as special cases the log-normal and gamma distributions, which have been widely used to characterize regular spiking neurons. In numeric simulations we investigated how serial correlation affects the variance of the neural spike count. We show that the experimentally confirmed negative correlation reduces single-neuron variability, as quantified by the Fano factor, by up to 50%, which favors the transmission of a rate code. We argue that the feature of a negative serial correlation is likely to be common to the class of spike-frequency-adapting neurons and that it might have been largely overlooked in extracellular single-unit recordings due to spike sorting errors.

  17. Missing data and technical variability in single-cell RNA-sequencing experiments.

    Science.gov (United States)

    Hicks, Stephanie C; Townes, F William; Teng, Mingxiang; Irizarry, Rafael A

    2017-11-06

    Until recently, high-throughput gene expression technology, such as RNA-Sequencing (RNA-seq) required hundreds of thousands of cells to produce reliable measurements. Recent technical advances permit genome-wide gene expression measurement at the single-cell level. Single-cell RNA-Seq (scRNA-seq) is the most widely used and numerous publications are based on data produced with this technology. However, RNA-seq and scRNA-seq data are markedly different. In particular, unlike RNA-seq, the majority of reported expression levels in scRNA-seq are zeros, which could be either biologically-driven, genes not expressing RNA at the time of measurement, or technically-driven, genes expressing RNA, but not at a sufficient level to be detected by sequencing technology. Another difference is that the proportion of genes reporting the expression level to be zero varies substantially across single cells compared to RNA-seq samples. However, it remains unclear to what extent this cell-to-cell variation is being driven by technical rather than biological variation. Furthermore, while systematic errors, including batch effects, have been widely reported as a major challenge in high-throughput technologies, these issues have received minimal attention in published studies based on scRNA-seq technology. Here, we use an assessment experiment to examine data from published studies and demonstrate that systematic errors can explain a substantial percentage of observed cell-to-cell expression variability. Specifically, we present evidence that some of these reported zeros are driven by technical variation by demonstrating that scRNA-seq produces more zeros than expected and that this bias is greater for lower expressed genes. In addition, this missing data problem is exacerbated by the fact that this technical variation varies cell-to-cell. Then, we show how this technical cell-to-cell variability can be confused with novel biological results. Finally, we demonstrate and discuss how batch

  18. A new approach to assessing intra-subject variability in single-subject designs

    Directory of Open Access Journals (Sweden)

    Katy Borodkin

    2014-04-01

    Full Text Available Research Aim: One of the methodological challenges of single-subject designs is accounting for intra-individual variability in performance, which is commonly assessed by applying the same testing materials on multiple sessions (McReynolds & Thompson, 1986. This approach might be less feasible in some individuals, such as bilingual speakers with aphasia, who would need to be tested, using the same materials, on several occasions within as well as across languages. Repetitive exposure to the same testing materials can increase practice effects and further reduce the validity of the testing. In the present study, we explored an alternative approach to measuring stability in performance by comparing the use of identical vs. different (but comparable testing materials. Method: Participants were five monolinguals with non-fluent aphasia following a single left CVA. Participants performed an action-naming task and two narrative tasks: a picture sequence and a personal narrative. There were two testing times, several weeks apart using identical materials. Each testing time included three consecutive sessions, using different materials. Action-naming performance was assessed by the number of correct verbs produced. The verbal output in the narrative tasks was scored for amount (number of utterances, grammaticality (percentage of grammatical sentences, and verb diversity (number of different verbs. Pearson correlation coefficients (r were computed to establish the intra-subject variability across testing times and across sessions. The magnitude of the correlations was evaluated using published guidelines (Strauss, Sherman, & Spreen, 2006. Results: For action naming, the correlations ranged from high to very high (.87 to .98 across testing times and from adequate to very high across sessions (.74 to .96. For the narrative tasks, the correlations between the number of utterances across testing times ranged from adequate to very high (.76 to .97 and from low

  19. Long Latency and High Variability in Accuracy-Speed of Prosaccades in Alzheimer’s Disease at Mild to Moderate Stage

    Directory of Open Access Journals (Sweden)

    Qing Yang

    2011-10-01

    Full Text Available Background: Studying saccades is a useful tool to investigate brain function. There is still controversy regarding deficits in prosaccades in Alzheimer’s disease (AD, and a study of saccades in subjects with mild cognitive impairment (MCI has not been published to date. Methods: We examined horizontal saccades in 10 healthy elderly, and 9 MCI and 9 AD patients. Two tasks were used: gap (fixation target extinguishes prior to target onset and overlap (fixation stays on after target onset. Eye movements were recorded with the Skalar IRIS system. Results: (1 Latencies were shorter in the gap than in the overlap task (a gap effect in all three groups of subjects: healthy elderly, MCI and AD; (2 for both tasks, latency of saccades was longer for AD patients than for healthy and MCI subjects, and (3 accuracy and mean velocity were normal in MCI and AD subjects, however, variability in accuracy-speed was higher for AD patients than for healthy and MCI subjects in the overlap task. Conclusions: Abnormalities in reaction time and accuracy-speed variability reflect deficits in cerebral areas involved in the triggering and execution of saccades; a saccade test can be useful to follow up the evolution of MCI subjects as some of them may develop AD disease.

  20. Wind Turbine Diagnosis under Variable Speed Conditions Using a Single Sensor Based on the Synchrosqueezing Transform Method.

    Science.gov (United States)

    Guo, Yanjie; Chen, Xuefeng; Wang, Shibin; Sun, Ruobin; Zhao, Zhibin

    2017-05-18

    The gearbox is one of the key components in wind turbines. Gearbox fault signals are usually nonstationary and highly contaminated with noise. The presence of amplitude-modulated and frequency-modulated (AM-FM) characteristics compound the difficulty of precise fault diagnosis of wind turbines, therefore, it is crucial to develop an effective fault diagnosis method for such equipment. This paper presents an improved diagnosis method for wind turbines via the combination of synchrosqueezing transform and local mean decomposition. Compared to the conventional time-frequency analysis techniques, the improved method which is performed in non-real-time can effectively reduce the noise pollution of the signals and preserve the signal characteristics, and hence is suitable for the analysis of nonstationary signals with high noise. This method is further validated by simulated signals and practical vibration data measured from a 1.5 MW wind turbine. The results confirm that the proposed method can simultaneously control the noise and increase the accuracy of time-frequency representation.

  1. Computer program for analysis of high speed, single row, angular contact, spherical roller bearing, SASHBEAN. Volume 2: Mathematical formulation and analysis

    Science.gov (United States)

    Aggarwal, Arun K.

    1993-01-01

    Spherical roller bearings have typically been used in applications with speeds limited to about 5000 rpm and loads limited for operation at less than about 0.25 million DN. However, spherical roller bearings are now being designed for high load and high speed applications including aerospace applications. A computer program, SASHBEAN, was developed to provide an analytical tool to design, analyze, and predict the performance of high speed, single row, angular contact (including zero contact angle), spherical roller bearings. The material presented is the mathematical formulation and analytical methods used to develop computer program SASHBEAN. For a given set of operating conditions, the program calculates the bearings ring deflections (axial and radial), roller deflections, contact areas stresses, depth and magnitude of maximum shear stresses, axial thrust, rolling element and cage rotational speeds, lubrication parameters, fatigue lives, and rates of heat generation. Centrifugal forces and gyroscopic moments are fully considered. The program is also capable of performing steady-state and time-transient thermal analyses of the bearing system.

  2. Flexible reaction norms to environmental variables along the migration route and the significance of stopover duration for total speed of migration in a songbird migrant.

    Science.gov (United States)

    Schmaljohann, Heiko; Lisovski, Simeon; Bairlein, Franz

    2017-01-01

    Predicting the consequences of continuing anthropogenic changes in the environment for migratory behaviours such as phenology remains a major challenge. Predictions remain particularly difficult, because our knowledge is based on studies from single-snapshot observations at specific stopover sites along birds' migration routes. However, a general understanding on how birds react to prevailing environmental conditions, e.g. their 'phenotypic reaction norm', throughout the annual cycle and along their entire migration routes is required to fully understand how migratory birds respond to rapid environmental change. Here, we provide direct evidence that northern wheatears (Oenanthe oenanthe) from a breeding population in Alaska adjusted their probability to resume migration as well as the distance covered per night, i.e. travel speed, to large-scale environmental conditions experienced along their 15,000 km migratory route on both northwards and southwards migrations. These adjustments were found to be flexible in space and time. At the beginning of autumn migration, northern wheatears showed high departure probabilities and high travel speeds at low surface air temperatures, while far away from Alaska both traits decreased with increasing air temperatures. In spring, northern wheatears increasingly exploited flow assistance with season, which is likely a behavioural adjustment to speed up migration by increasing the distance travelled per night. Furthermore, the variation in total stopover duration but not in travel speed had a significant effect on the total speed of migration, indicating the prime importance of total stopover duration in the overall phenology of bird migration. Northern wheatears from Alaska provide evidence that the phenotypic reaction norm to a set of environmental conditions cannot be generalized to universal and persistent behavioural reaction pattern across entire migratory pathways. This highlights the importance of full annual-cycle studies

  3. Generalized P-phased Regression Estimators with Single and Two Auxiliary Variables

    Directory of Open Access Journals (Sweden)

    Farhan Hameed

    2017-09-01

    Full Text Available Multiphase sampling has been the concept not being utilized is estimation of ratio and regression estimator widely. In the recent study we have proposed new dimension of sampling survey of estimations by proposing two generalized p-phase regression estimators with single and two auxiliary variables for estimating population mean. The proposed estimators are the generalized p-phase cases of Hanif et al (2015 and Hanif (2007 respectively. Both the estimators from which we took motivation are now special cases of our proposed estimators. We have derived unbiasedness, expression of Mean Square Errors along with family of estimators based upon p-phased generalization. We have derived expression of MSE in such a way that these expression can be used to obtained results for every phase we desire. By conducting empirical study on proposed estimators we have shown many situation in which MSE can be reduced by increasing number of phases. Hence, our study will open new horizon in the field of multiphase sampling where a lot of challenges are waiting to be resolved by proposing new estimators for phases above 2nd phase.

  4. Display of single-chain variable fragments on bacteriophage MS2 virus-like particles.

    Science.gov (United States)

    Lino, Christopher A; Caldeira, Jerri C; Peabody, David S

    2017-02-13

    Virus-like particles (VLPs) of the RNA bacteriophage MS2 have many potential applications in biotechnology. MS2 VLPs provide a platform for peptide display and affinity selection (i.e. biopanning). They are also under investigation as vehicles for targeted drug delivery, using display of receptor-specific peptides or nucleic acid aptamers to direct their binding to specific cell-surface receptors. However, there are few molecules more suited to the precise targeting and binding of a cellular receptor than antibodies. Here we describe a strategy for display of four different functional single-chain variable fragments (scFvs) on the surface of the MS2 VLP. Each scFv is validated both for its presence on the surface of the VLP and for its ability to bind its cognate antigen. This work demonstrates the suitability of the MS2 VLP platform to display genetically fused scFvs, allowing for many potential applications of these VLPs and paving the way for future work with libraries of scFvs displayed in a similar manner on the VLP surface. These libraries can then be biopanned and novel scFv binders to targets can be readily discovered.

  5. Optical coherence elastography based on high speed imaging of single-hot laser-induced acoustic waves at 16 kHz frame rate

    Science.gov (United States)

    Song, Shaozhen; Hsieh, Bao-Yu; Wei, Wei; Shen, Tueng; Pelivanov, Ivan; O'Donnell, Matthew; Wang, Ruikang K.

    2016-03-01

    Shear wave OCE (SW-OCE) is a novel technique that relies on the detection of the localized shear wave speed to map tissue elasticity. In this study, we demonstrate high speed imaging to capture single-shot transient shear wave propagation for SW-OCE. The fast imaging speed is achieved using a Fourier domain mode-locked (FDML) high-speed swept-source OCT (SS-OCT) system. The frame rate of shear wave imaging is 16 kHz, at an A-line rate of ~1.62 MHz, enabling the detection of high-frequency shear waves up to 8 kHz in bandwidth. Several measures are taken to improve the phase-stability of the SS-OCT system, and the measured displacement sensitivity is ~10 nanometers. To facilitate non-contact elastography, shear waves are generated with the photo-thermal effect using an ultra-violet pulsed laser. High frequency shear waves launched by the pulsed laser contain shorter wavelengths and carry rich localized elasticity information. Benefiting from single-shot acquisition, each SWI scan only takes 2.5 milliseconds, and the reconstruction of the elastogram can be performed in real-time with ~20 Hz refresh rate. SW-OCE measurements are demonstrated on porcine cornea ex vivo. This study is the first demonstration of an all-optical method to perform real-time 3D SW-OCE. It is hoped that this technique will be applicable in the clinic to obtain high-resolution localized quantitative measurements of tissue biomechanical properties.

  6. The Impacts of Wind Speed Trends and 30-Year Variability in Relation to Hydroelectric Reservoir Inflows on Wind Power in the Pacific Northwest.

    Directory of Open Access Journals (Sweden)

    Benjamin D Cross

    Full Text Available In hydroelectric dominated systems, the value and benefits of energy are higher during extended dry periods and lower during extended or extreme wet periods. By accounting for regional and temporal differences in the relationship between wind speed and reservoir inflow behavior during wind farm site selection, the benefits of energy diversification can be maximized. The goal of this work was to help maximize the value of wind power by quantifying the long-term (30-year relationships between wind speed and streamflow behavior, using British Columbia (BC and the Pacific Northwest (PNW as a case study. Clean energy and self-sufficiency policies in British BC make the benefits of increased generation during low streamflow periods particularly large. Wind density (WD estimates from a height of 10m (North American Regional Reanalysis, NARR were correlated with cumulative usable inflows (CUI for BC (collected from BC Hydro for 1979-2010. The strongest WD-CUI correlations were found along the US coast (r ~0.55, whereas generally weaker correlations were found in northern regions, with negative correlations (r ~ -0.25 along BC's North Coast. Furthermore, during the lowest inflow years, WD anomalies increased by up to 40% above average values for the North Coast. Seasonally, high flows during the spring freshet were coincident with widespread negative WD anomalies, with a similar but opposite pattern for low inflow winter months. These poorly or negatively correlated sites could have a moderating influence on climate related variability in provincial electricity supply, by producing greater than average generation in low inflow years and reduced generation in wet years. Wind speed and WD trends were also analyzed for all NARR grid locations, which showed statistically significant positive trends for most of the PNW and the largest increases along the Pacific Coast.

  7. The Impacts of Wind Speed Trends and 30-Year Variability in Relation to Hydroelectric Reservoir Inflows on Wind Power in the Pacific Northwest.

    Science.gov (United States)

    Cross, Benjamin D; Kohfeld, Karen E; Bailey, Joseph; Cooper, Andrew B

    2015-01-01

    In hydroelectric dominated systems, the value and benefits of energy are higher during extended dry periods and lower during extended or extreme wet periods. By accounting for regional and temporal differences in the relationship between wind speed and reservoir inflow behavior during wind farm site selection, the benefits of energy diversification can be maximized. The goal of this work was to help maximize the value of wind power by quantifying the long-term (30-year) relationships between wind speed and streamflow behavior, using British Columbia (BC) and the Pacific Northwest (PNW) as a case study. Clean energy and self-sufficiency policies in British BC make the benefits of increased generation during low streamflow periods particularly large. Wind density (WD) estimates from a height of 10m (North American Regional Reanalysis, NARR) were correlated with cumulative usable inflows (CUI) for BC (collected from BC Hydro) for 1979-2010. The strongest WD-CUI correlations were found along the US coast (r ~0.55), whereas generally weaker correlations were found in northern regions, with negative correlations (r ~ -0.25) along BC's North Coast. Furthermore, during the lowest inflow years, WD anomalies increased by up to 40% above average values for the North Coast. Seasonally, high flows during the spring freshet were coincident with widespread negative WD anomalies, with a similar but opposite pattern for low inflow winter months. These poorly or negatively correlated sites could have a moderating influence on climate related variability in provincial electricity supply, by producing greater than average generation in low inflow years and reduced generation in wet years. Wind speed and WD trends were also analyzed for all NARR grid locations, which showed statistically significant positive trends for most of the PNW and the largest increases along the Pacific Coast.

  8. Modeling the uptake of neutral organic chemicals on XAD passive air samplers under variable temperatures, external wind speeds and ambient air concentrations (PAS-SIM).

    Science.gov (United States)

    Armitage, James M; Hayward, Stephen J; Wania, Frank

    2013-01-01

    The main objective of this study was to evaluate the performance and demonstrate the utility of a fugacity-based model of XAD passive air samplers (XAD-PAS) designed to simulate the uptake of neutral organic chemicals under variable temperatures, external wind speeds and ambient air concentrations. The model (PAS-SIM) simulates the transport of the chemical across the air-side boundary layer and within the sampler medium, which is segmented into a user-defined number of thin layers. Model performance was evaluated using data for polychlorinated biphenyls (PCBs) and polycyclic aromatic hydrocarbons (PAHs) from a field calibration study (i.e., active and XAD-PAS data) conducted in Egbert, Ontario, Canada. With some exceptions, modeled PAS uptake curves are in good agreement with the empirical PAS data. The results are highly encouraging, given the uncertainty in the active air sampler data used as input and other uncertainties related to model parametrization (e.g., sampler-air partition coefficients, the influence of wind speed on sampling rates). The study supports the further development and evaluation of the PAS-SIM model as a diagnostic (e.g., to aid interpretation of calibration studies and monitoring data) and prognostic (e.g., to inform design of future passive air sampling campaigns) tool.

  9. The Relation between Executive Functioning, Reaction Time, Naming Speed, and Single Word Reading in Children with Typical Development and Language Impairments

    Science.gov (United States)

    Messer, David; Henry, Lucy A.; Nash, Gilly

    2016-01-01

    Background: Few investigations have examined the relationship between a comprehensive range of executive functioning (EF) abilities and reading. Aims: Our investigation identified components of EF that independently predicted single word reading, and determined whether their predictive role remained when additional variables were included in the…

  10. Improving estimates of regional vegetation: Using pre-settlement vegetation data and variable wind speed to quantify pollen dispersal and source area

    Science.gov (United States)

    Burke, K. D.; Goring, S. J.; Williams, J. W.; Holloway, T.

    2014-12-01

    Pollen-based vegetation reconstructions are the primary source of information about spatio-temporal trends in vegetation dynamics at timescales of centuries to millennia. Pollen samples from individual lakes, bogs, and small hollows provide information about the vegetation within their respective source areas, which when mapped together generate vegetation reconstructions across spatial and temporal scales. Climate histories also can be reconstructed using empirical taxa-climate relationships. A fundamental need in these reconstructions is to estimate the pollen source area and account for the effects of intertaxonomic differences in pollen production and dispersal. Mechanistic pollen-vegetation models have continuously evolved for nearly a century, with recent advances culminating in the Landscape Reconstruction Algorithm. However, most applications of the LRA do not account for anisotropies in pollen source area introduced by atmospheric variations. Given that wind direction is often anisotropic and variable we investigate the effects of wind speed, wind direction, and a simple treatment of wet versus dry deposition on vegetation reconstruction from pollen. We obtained long term estimates of dominant wind patterns using North American Regional Reanalysis (NARR) weather data for 1979-2012. However, because contemporary vegetation and pollen distributions have been heavily affected by Euro-American land use, we use pre-settlement (ca. 1810-1904) forest composition across the prairie-forest ecotone in the upper Midwestern United States and a dataset of settlement-era pollen samples compiled by the PalEON project to model pollen-vegetation relationships. Comparisons of our modelled results with those of a unidirectional, homogenous model shows a substantial effect of regionally varying winds on vegetation reconstructions. In addition to variable wind speed and direction, other atmospheric effects including precipitation and instability play an important role in

  11. Very High Fuel Economy, Heavy Duty, Constant Speed, Truck Engine Optimized Via Unique Energy Recovery Turbines and Facilitated High Efficiency Continuously Variable Drivetrain

    Energy Technology Data Exchange (ETDEWEB)

    Bahman Habibzadeh

    2010-01-31

    The project began under a corporative agreement between Mack Trucks, Inc and the Department of Energy starting from September 1, 2005. The major objective of the four year project is to demonstrate a 10% efficiency gain by operating a Volvo 13 Litre heavy-duty diesel engine at a constant or narrow speed and coupled to a continuously variable transmission. The simulation work on the Constant Speed Engine started on October 1st. The initial simulations are aimed to give a basic engine model for the VTEC vehicle simulations. Compressor and turbine maps are based upon existing maps and/or qualified, realistic estimations. The reference engine is a MD 13 US07 475 Hp. Phase I was completed in May 2006 which determined that an increase in fuel efficiency for the engine of 10.5% over the OICA cycle, and 8.2% over a road cycle was possible. The net increase in fuel efficiency would be 5% when coupled to a CVT and operated over simulated highway conditions. In Phase II an economic analysis was performed on the engine with turbocompound (TC) and a Continuously Variable Transmission (CVT). The system was analyzed to determine the payback time needed for the added cost of the TC and CVT system. The analysis was performed by considering two different production scenarios of 10,000 and 60,000 units annually. The cost estimate includes the turbocharger, the turbocompound unit, the interstage duct diffuser and installation details, the modifications necessary on the engine and the CVT. Even with the cheapest fuel and the lowest improvement, the pay back time is only slightly more than 12 months. A gear train is necessary between the engine crankshaft and turbocompound unit. This is considered to be relatively straight forward with no design problems.

  12. An Approach to Speed up Single-Frequency PPP Convergence with Quad-Constellation GNSS and GIM.

    Science.gov (United States)

    Cai, Changsheng; Gong, Yangzhao; Gao, Yang; Kuang, Cuilin

    2017-06-06

    The single-frequency precise point positioning (PPP) technique has attracted increasing attention due to its high accuracy and low cost. However, a very long convergence time, normally a few hours, is required in order to achieve a positioning accuracy level of a few centimeters. In this study, an approach is proposed to accelerate the single-frequency PPP convergence by combining quad-constellation global navigation satellite system (GNSS) and global ionospheric map (GIM) data. In this proposed approach, the GPS, GLONASS, BeiDou, and Galileo observations are directly used in an uncombined observation model and as a result the ionospheric and hardware delay (IHD) can be estimated together as a single unknown parameter. The IHD values acquired from the GIM product and the multi-GNSS differential code bias (DCB) product are then utilized as pseudo-observables of the IHD parameter in the observation model. A time varying weight scheme has also been proposed for the pseudo-observables to gradually decrease its contribution to the position solutions during the convergence period. To evaluate the proposed approach, datasets from twelve Multi-GNSS Experiment (MGEX) stations on seven consecutive days are processed and analyzed. The numerical results indicate that the single-frequency PPP with quad-constellation GNSS and GIM data are able to reduce the convergence time by 56%, 47%, 41% in the east, north, and up directions compared to the GPS-only single-frequency PPP.

  13. Thermohaline circulation: Energetics and variability in a single-hemisphere basin model

    Science.gov (United States)

    Huang, Rui Xin

    1994-06-01

    A series of numerical experiments have been carried out to explore the variability of the thermohaline circulation and the energetics of halocline catastrophe. It is found that when the amplitude of surface freshwater flux is smaller than a critical value, the thermohaline circulation is in a thermal mode, with deep water formed in the north. When the freshwater flux amplitude is supercritical, the thermohaline circulation does not reach a single steady state. Instead, the model ocean is in a continuous transition between a slow, quasi-steady saline mode and an energetic, unsteady thermal mode. For cases with no wind stress, the saline mode is characterized by sinking along the equator. For cases with wind stress, the saline mode is characterized by intermediate water formation at midlatitude. During the saline mode phase, the deep water gradually becomes warm and salty. Thus at the end of the saline mode phase, within the northern basin there is cold and relatively fresh water lying on top of warm and salty water. Such a vertical structure is potentially very unstable because small perturbations can grow, supported by the release of potential energy during strong cooling. A quantity called the diabatic available potential energy index is introduced as an indicator for such convective instability. Thus a final equilibrium in the saline mode cannot be reached; instead, the model ocean flips to a very energetic thermal mode in which violent overturning destroys the vertical stratification. After the energy is released, the saline cell supported by precipitation in the subpolar basin advances southward and the model ocean returns to the saline mode. The whole cycle will be repeated.

  14. Isolation and characterization of anti c-met single chain fragment variable (scFv) antibodies.

    Science.gov (United States)

    Qamsari, Elmira Safaie; Sharifzadeh, Zahra; Bagheri, Salman; Riazi-Rad, Farhad; Younesi, Vahid; Abolhassani, Mohsen; Ghaderi, Sepideh Safaei; Baradaran, Behzad; Somi, Mohammad Hossein; Yousefi, Mehdi

    2017-12-01

    The receptor tyrosine kinase (RTK) Met is the cell surface receptor for hepatocyte growth factor (HGF) involved in invasive growth programs during embryogenesis and tumorgenesis. There is compelling evidence suggesting important roles for c-Met in colorectal cancer proliferation, migration, invasion, angiogenesis, and survival. Hence, a molecular inhibitor of an extracellular domain of c-Met receptor that blocks c-Met-cell surface interactions could be of great thera-peutic importance. In an attempt to develop molecular inhibitors of c-Met, single chain variable fragment (scFv) phage display libraries Tomlinson I + J against a specific synthetic oligopeptide from the extracellular domain of c-Met receptor were screened; selected scFv were then characterized using various immune techniques. Three c-Met specific scFv (ES1, ES2, and ES3) were selected following five rounds of panning procedures. The scFv showed specific binding to c-Met receptor, and significantly inhibited proliferation responses of a human colorectal carcinoma cell line (HCT-116). Moreover, anti- apoptotic effects of selected scFv antibodies on the HCT-116 cell line were also evaluated using Annexin V/PI assays. The results demonstrated rates of apoptotic cell death of 46.0, 25.5, and 37.8% among these cells were induced by use of ES1, ES2, and ES3, respectively. The results demonstrated ability to successfully isolate/char-acterize specific c-Met scFv that could ultimately have a great therapeutic potential in immuno-therapies against (colorectal) cancers.

  15. Variable myopathic presentation in a single family with novel skeletal RYR1 mutation.

    Directory of Open Access Journals (Sweden)

    Ruben Attali

    Full Text Available We describe an autosomal recessive heterogeneous congenital myopathy in a large consanguineous family. The disease is characterized by variable severity, progressive course in 3 of 4 patients, myopathic face without ophthalmoplegia and proximal muscle weakness. Absence of cores was noted in all patients. Genome wide linkage analysis revealed a single locus on chromosome 19q13 with Zmax = 3.86 at θ = 0.0 and homozygosity of the polymorphic markers at this locus in patients. Direct sequencing of the main candidate gene within the candidate region, RYR1, was performed. A novel homozygous A to G nucleotide substitution (p.Y3016C within exon 60 of the RYR1 gene was found in patients. ARMS PCR was used to screen for the mutation in all available family members and in an additional 150 healthy individuals. This procedure confirmed sequence analysis and did not reveal the A to G mutation (p.Y3016C in 300 chromosomes from healthy individuals. Functional analysis on EBV immortalized cell lines showed no effect of the mutation on RyR1 pharmacological activation or the content of intracellular Ca(2+ stores. Western blot analysis demonstrated a significant reduction of the RyR1 protein in the patient's muscle concomitant with a reduction of the DHPRα1.1 protein. This novel mutation resulting in RyR1 protein decrease causes heterogeneous clinical presentation, including slow progression course and absence of centrally localized cores on muscle biopsy. We suggest that RYR1 related myopathy should be considered in a wide variety of clinical and pathological presentation in childhood myopathies.

  16. Variable myopathic presentation in a single family with novel skeletal RYR1 mutation.

    Science.gov (United States)

    Attali, Ruben; Aharoni, Sharon; Treves, Susan; Rokach, Ori; Becker Cohen, Michal; Fellig, Yakov; Straussberg, Rachel; Dor, Talya; Daana, Muhannad; Mitrani-Rosenbaum, Stella; Nevo, Yoram

    2013-01-01

    We describe an autosomal recessive heterogeneous congenital myopathy in a large consanguineous family. The disease is characterized by variable severity, progressive course in 3 of 4 patients, myopathic face without ophthalmoplegia and proximal muscle weakness. Absence of cores was noted in all patients. Genome wide linkage analysis revealed a single locus on chromosome 19q13 with Zmax = 3.86 at θ = 0.0 and homozygosity of the polymorphic markers at this locus in patients. Direct sequencing of the main candidate gene within the candidate region, RYR1, was performed. A novel homozygous A to G nucleotide substitution (p.Y3016C) within exon 60 of the RYR1 gene was found in patients. ARMS PCR was used to screen for the mutation in all available family members and in an additional 150 healthy individuals. This procedure confirmed sequence analysis and did not reveal the A to G mutation (p.Y3016C) in 300 chromosomes from healthy individuals. Functional analysis on EBV immortalized cell lines showed no effect of the mutation on RyR1 pharmacological activation or the content of intracellular Ca(2+) stores. Western blot analysis demonstrated a significant reduction of the RyR1 protein in the patient's muscle concomitant with a reduction of the DHPRα1.1 protein. This novel mutation resulting in RyR1 protein decrease causes heterogeneous clinical presentation, including slow progression course and absence of centrally localized cores on muscle biopsy. We suggest that RYR1 related myopathy should be considered in a wide variety of clinical and pathological presentation in childhood myopathies.

  17. Impact of injection speed and volume on perceived pain during subcutaneous injections into the abdomen and thigh: a single-centre, randomized controlled trial.

    Science.gov (United States)

    Heise, T; Nosek, L; Dellweg, S; Zijlstra, E; Præstmark, K A; Kildegaard, J; Nielsen, G; Sparre, T

    2014-10-01

    The aim of this study was to assess pain associated with subcutaneous injection into the abdomen and thigh of different combinations of injection speeds and volumes. The study was a single-centre, one-visit, double-blinded, randomized controlled trial in 82 adults with type 1 or type 2 diabetes receiving daily injections of insulin or glucagon-like peptide-1 (GLP-1) agonists. Participants received 17 subcutaneous injections (12 in abdomen, 5 in thigh) of saline at different injection speeds (150, 300 and 450 µl/s), with different volumes (400, 800, 1200 and 1600 µl), and two needle insertions without any injection. Pain was evaluated on a 100-mm visual analogue scale (VAS) (0 mm no pain, 100 mm worst pain) and on a yes/no scale for pain acceptability. Injection speed had no impact on injection pain (p = 0.833). Injection of larger volumes caused significantly more pain [VAS least square mean differences 1600 vs. 400 µl, 7 · 2 mm (95% confidence interval - CI; 4.6-9.7; p pain occurred in the thigh versus the abdomen [9.0 mm (6.7-11.3; p pain, whereas higher injection volumes caused more pain. The results of this study may be of value for guiding patients to use the appropriate injection site and technique to reduce their injection pain. Furthermore, these findings may have important implications for the development of new injection devices and drug formulations for clinical practice. © 2014 John Wiley & Sons Ltd.

  18. Effect of speed endurance training and reduced training volume on running economy and single muscle fiber adaptations in trained runners

    DEFF Research Database (Denmark)

    Skovgaard, Casper; Christiansen, Danny; Christensen, Peter Møller

    2018-01-01

    The aim of the present study was to examine whether improved running economy with a period of speed endurance training and reduced training volume could be related to adaptations in specific muscle fibers. Twenty trained male (n = 14) and female (n = 6) runners (maximum oxygen consumption (VO2 -max.......05) in ST muscle fibers, and sarcoplasmic reticulum calcium ATPase 1 (SERCA1) was lower (P economy at 60% vVO2 -max (11.6 ± 0.2 km/h) and at v10-km (13.7 ± 0.3 km/h) was ~2% better (P ....3 ± 0.3 vs. 18.9 ± 0.3 km/h) after than before the intervention. Thus, improved running economy with intense training may be related to changes in expression of proteins linked to energy consuming processes in primarily ST muscle fibers....

  19. Estimation of Hedonic Single-Family House Price Function Considering Neighborhood Effect Variables

    Directory of Open Access Journals (Sweden)

    Chihiro Shimizu

    2014-05-01

    Full Text Available In the formulation of hedonic models, in addition to locational factors and building structures which affect the house prices, the generation of the omitted variable bias is thought to occur in cases when local environmental variables and the individual characteristics of house buyers are not taken into consideration. However, since it is difficult to obtain local environmental information in a small neighborhood unit and to observe individual characteristics of house buyers, these variables have not been sufficiently considered in previous studies. We demonstrated that non-negligible levels of omitted variable bias are generated if these variables are not considered.

  20. A single position loop control strategy for high-speed voice coil motor based on active disturbance rejection control

    DEFF Research Database (Denmark)

    Wang, Zhongxu; Wang, Huai; Li, Yong

    2017-01-01

    To improve the dynamic performance of voice coil motor, a single position loop control method based on active disturbance rejection control is proposed in this paper. The feedback law is sufficiently analyzed and well designed considering both sinusoidal input and step reference input. Moreover...

  1. Towards single-valued polylogarithms in two variables for the seven-point remainder function in multi-Regge kinematics

    Directory of Open Access Journals (Sweden)

    Johannes Broedel

    2017-02-01

    Full Text Available We investigate single-valued polylogarithms in two complex variables, which are relevant for the seven-point remainder function in N=4 super-Yang–Mills theory in the multi-Regge regime. After constructing these two-dimensional polylogarithms, we determine the leading logarithmic approximation of the seven-point remainder function up to and including five loops.

  2. A 12-bit high-speed column-parallel two-step single-slope analog-to-digital converter (ADC) for CMOS image sensors.

    Science.gov (United States)

    Lyu, Tao; Yao, Suying; Nie, Kaiming; Xu, Jiangtao

    2014-11-17

    A 12-bit high-speed column-parallel two-step single-slope (SS) analog-to-digital converter (ADC) for CMOS image sensors is proposed. The proposed ADC employs a single ramp voltage and multiple reference voltages, and the conversion is divided into coarse phase and fine phase to improve the conversion rate. An error calibration scheme is proposed to correct errors caused by offsets among the reference voltages. The digital-to-analog converter (DAC) used for the ramp generator is based on the split-capacitor array with an attenuation capacitor. Analysis of the DAC's linearity performance versus capacitor mismatch and parasitic capacitance is presented. A prototype 1024 × 32 Time Delay Integration (TDI) CMOS image sensor with the proposed ADC architecture has been fabricated in a standard 0.18 μm CMOS process. The proposed ADC has average power consumption of 128 μW and a conventional rate 6 times higher than the conventional SS ADC. A high-quality image, captured at the line rate of 15.5 k lines/s, shows that the proposed ADC is suitable for high-speed CMOS image sensors.

  3. Origins and characteristics of the threshold voltage variability of quasiballistic single-walled carbon nanotube field-effect transistors.

    Science.gov (United States)

    Cao, Qing; Han, Shu-jen; Penumatcha, Ashish V; Frank, Martin M; Tulevski, George S; Tersoff, Jerry; Haensch, Wilfried E

    2015-02-24

    Ultrascaled transistors based on single-walled carbon nanotubes are identified as one of the top candidates for future microprocessor chips as they provide significantly better device performance and scaling properties than conventional silicon technologies. From the perspective of the chip performance, the device variability is as important as the device performance for practical applications. This paper presents a systematic investigation on the origins and characteristics of the threshold voltage (VT) variability of scaled quasiballistic nanotube transistors. Analysis of experimental results from variable-temperature measurement as well as gate oxide thickness scaling studies shows that the random variation from fixed charges present on the oxide surface close to nanotubes dominates the VT variability of nanotube transistors. The VT variability of single-tube transistors has a figure of merit that is quantitatively comparable with that of current silicon devices; and it could be reduced with the adoption of improved device passivation schemes, which might be necessary for practical devices incorporating multiple nanotubes, whose area normalized VT variability becomes worse due to the synergic effects from the limited surface coverage of nanotubes and the nonlinearity of the device off-state leakage current, as predicted by the Monte Carlo simulation.

  4. A computational investigation of vibration attenuation of a rigid rotor turning at a variable speed by means of short magnetorheological dampers

    Directory of Open Access Journals (Sweden)

    Zapoměl J.

    2009-12-01

    Full Text Available Rotors of all rotating machines are always slightly imbalanced. When they rotate, the imbalance induces their lateral vibration and forces that are transmitted via the bearings into the foundations. These phenomena are significant if the rotor accelerates or decelerates and especially if it passes over the critical speeds. The vibration can be reduced if the rotor supports are equipped with damping elements. To achieve optimum performance of the damper, the damping effect must be controllable. At present time, semiactive magnetorheological squeeze film dampers are a subject of intensive research. They work on a principle of squeezing a thin film of magnetorheological liquid. If magnetic field is applied, the magnetorheological liquid starts to flow only if the shear stress between two neighbourhood layers exceeds a limit value which depends on intensity of the magnetic field. Its change enables to control the damping force. In the mathematical models, the magnetorheological liquid is usually considered as Bingham one. Application of the computer modelling method for analysis of rotors supported by rolling element bearings and magnetorheological squeeze film dampers and turning at variable angular speed requires to set up the equations of motion of the rotor and to develop a procedure for calculation of the damping force. Derivation of the equations of motion starts from the first and second impulse theorems. The pressure distribution in the thin lubricating film can be described by a Reynolds equation modified for the case of Bingham liquid. In cavitated areas, it is assumed that pressure of the medium remains constant. The hydraulic force acting on the rotor journal is then obtained by integration of the pressure distribution around the circumference and along the length of the damper. Applicability of the developed procedures was tested by means of computer simulations and influence of the control of the damping force on vibration of the rotor

  5. Bio-mathematical analysis for the peristaltic flow of single wall carbon nanotubes under the impact of variable viscosity and wall properties.

    Science.gov (United States)

    Shahzadi, Iqra; Sadaf, Hina; Nadeem, Sohail; Saleem, Anber

    2017-02-01

    The main objective of this paper is to study the Bio-mathematical analysis for the peristaltic flow of single wall carbon nanotubes under the impact of variable viscosity and wall properties. The right and the left walls of the curved channel possess sinusoidal wave that is travelling along the outer boundary. The features of the peristaltic motion are determined by using long wavelength and low Reynolds number approximation. Exact solutions are determined for the axial velocity and for the temperature profile. Graphical results have been presented for velocity profile, temperature and stream function for various physical parameters of interest. Symmetry of the curved channel is disturbed for smaller values of the curvature parameter. It is found that the altitude of the velocity profile increases for larger values of variable viscosity parameter for both the cases (pure blood as well as single wall carbon nanotubes). It is detected that velocity profile increases with increasing values of rigidity parameter. It is due to the fact that an increase in rigidity parameter decreases tension in the walls of the blood vessels which speeds up the blood flow for pure blood as well as single wall carbon nanotubes. Increase in Grashof number decreases the fluid velocity. This is due to the reason that viscous forces play a prominent role that's why increase in Grashof number decreases the velocity profile. It is also found that temperature drops for increasing values of nanoparticle volume fraction. Basically, higher thermal conductivity of the nanoparticles plays a key role for quick heat dissipation, and this justifies the use of the single wall carbon nanotubes in different situations as a coolant. Exact solutions are calculated for the temperature and the velocity profile. Symmetry of the curved channel is destroyed due to the curvedness for velocity, temperature and contour plots. Addition of single wall carbon nanotubes shows a decrease in fluid temperature. Trapping

  6. Evaluation of range and distortion tolerance for high Mach number transonic fan stages. Task 2: Performance of a 1500-foot-per-second tip speed transonic fan stage with variable geometry inlet guide vanes and stator

    Science.gov (United States)

    Bilwakesh, K. R.; Koch, C. C.; Prince, D. C.

    1972-01-01

    A 0.5 hub/tip radius ratio compressor stage consisting of a 1500 ft/sec tip speed rotor, a variable camber inlet guide vane and a variable stagger stator was designed and tested with undistorted inlet flow, flow with tip radial distortion, and flow with 90 degrees, one-per-rev, circumferential distortion. At the design speed and design IGV and stator setting the design stage pressure ratio was achieved at a weight within 1% of the design flow. Analytical results on rotor tip shock structure, deviation angle and part-span shroud losses at different operating conditions are presented. The variable geometry blading enabled efficient operation with adequate stall margin at the design condition and at 70% speed. Closing the inlet guide vanes to 40 degrees changed the speed-versus-weight flow relationship along the stall line and thus provided the flexibility of operation at off-design conditions. Inlet flow distortion caused considerable losses in peak efficiency, efficiency on a constant throttle line through design pressure ratio at design speed, stall pressure ratio, and stall margin at the 0 degrees IGV setting and high rotative speeds. The use of the 40 degrees inlet guide vane setting enabled partial recovery of the stall margin over the standard constant throttle line.

  7. Wind speed variability over the Canary Islands, 1948-2014: focusing on trend differences at the land-ocean interface and below-above the trade-wind inversion layer

    Science.gov (United States)

    Azorin-Molina, Cesar; Menendez, Melisa; McVicar, Tim R.; Acevedo, Adrian; Vicente-Serrano, Sergio M.; Cuevas, Emilio; Minola, Lorenzo; Chen, Deliang

    2017-08-01

    This study simultaneously examines wind speed trends at the land-ocean interface, and below-above the trade-wind inversion layer in the Canary Islands and the surrounding Eastern North Atlantic Ocean: a key region for quantifying the variability of trade-winds and its response to large-scale atmospheric circulation changes. Two homogenized data sources are used: (1) observed wind speed from nine land-based stations (1981-2014), including one mountain weather station (Izaña) located above the trade-wind inversion layer; and (2) simulated wind speed from two atmospheric hindcasts over ocean (i.e., SeaWind I at 30 km for 1948-2014; and SeaWind II at 15 km for 1989-2014). The results revealed a widespread significant negative trend of trade-winds over ocean for 1948-2014, whereas no significant trends were detected for 1989-2014. For this recent period wind speed over land and ocean displayed the same multi-decadal variability and a distinct seasonal trend pattern with a strengthening (late spring and summer; significant in May and August) and weakening (winter-spring-autumn; significant in April and September) of trade-winds. Above the inversion layer at Izaña, we found a predominance of significant positive trends, indicating a decoupled variability and opposite wind speed trends when compared to those reported in boundary layer. The analysis of the Trade Wind Index (TWI), the North Atlantic Oscillation Index (NAOI) and the Eastern Atlantic Index (EAI) demonstrated significant correlations with the wind speed variability, revealing that the correlation patterns of the three indices showed a spatio-temporal complementarity in shaping wind speed trends across the Eastern North Atlantic.

  8. Extending the Linear Modulation Range to the Full Base Speed Using a Single DC-Link Multilevel Inverter With Capacitor-Fed H-Bridges for IM Drives

    DEFF Research Database (Denmark)

    Rahul, Arun; Pramanick, Sumit; Kaarthik, R. Sudharshan

    2017-01-01

    In this paper, a new space vector pulse width modulation method to extend the linear modulation range of a cascaded five level inverter topology with a single dc supply is presented. Using this method, the inverter can be controlled linearly and the peak phase fundamental output voltage...... of the inverter can be increased from 0.577 to 0.637Vdc without increasing the dc bus voltage and without exceeding the induction motor voltage rating. This new technique makes use of cascaded inverter pole voltage redundancy and property of the space vector structure for its operation. Using this, the induction...... motor drive can be operated till the full speed range (0-50 Hz) with the elimination of lower order harmonics in the phase voltage and phase current. The five-level topology presented in this paper is realized by cascading a two-level inverter and two full bridge modules with floating capacitors...

  9. Energy-optimal speed control of fans and compressor in a refrigeration system

    DEFF Research Database (Denmark)

    Jakobsen, Arne; Rasmussen, Bjarne D.

    1998-01-01

    Use of variable speed compressors and variable speed fans for both the evaporator and the condenser makes the refrigeration system more flexible, adds to the degree of freedom of the control system and therefore makes it possible to (on-line) optimise the various speeds involved. Say, for example...... that the cooling capacity must be increased due to increased cooling load. This can be done by increasing the compressor speed and/or the evaporator fan speed and also to some extent by increasing the condenser fan speed. The general control problem is to obtain the desired temperature of the cooled air, while...... choosing the combination of compressor speed, evaporator fan and condenser fan speeds which minimises the total power consumption of the motors involved. A system and integrated control viewpoint is necessary as a “component-oriented” and “single-loop-control” approach will not lead to the discovery...

  10. FRAS1-related extracellular matrix 3 (FREM3 single-nucleotide polymorphism effects on gene expression, amygdala reactivity and perceptual processing speed

    Directory of Open Access Journals (Sweden)

    Yuliya eNikolova

    2015-09-01

    Full Text Available The A allele of the Fras1-related extracellular matrix protein 3 (FREM3 rs7676614 single nucleotide polymorphism (SNP was linked to major depressive disorder (MDD in an early genome-wide association study (GWAS, and to symptoms of psychomotor retardation in a follow-up investigation. In line with significant overlap between age- and depression-related molecular pathways, parallel work has shown that FREM3 expression in postmortem human brain decreases with age. Here we probe the effect of rs7676614 on amygdala reactivity and perceptual processing speed, both of which are altered in depression and aging. Amygdala reactivity was assessed using a face-matching BOLD fMRI paradigm in 365 Caucasian participants in the Duke Neurogenetics Study (192 women, mean age 19.7±1.2. Perceptual processing speed was indexed by reaction times in the same task and the Trails Making Test (TMT. The effect of rs7676614 on FREM3 mRNA brain expression levels was probed in a postmortem cohort of 169 Caucasian individuals (44 women, mean age 50.8±14.9. The A allele of rs7676614 was associated with blunted amygdala reactivity to faces, slower reaction times in the face-matching condition (p<0.04, as well as marginally slower performance on TMT Part B (p=0.056. In the postmortem cohort, the T allele of rs6537170 (proxy for the rs7676614 A allele, was associated with trend-level reductions in gene expression in Brodmann areas 11 and 47 (p=0.066, reminiscent of patterns characteristic of older age. The low-expressing allele of another FREM3 SNP (rs1391187 was similarly associated with reduced amygdala reactivity and slower TMT Part B speed, in addition to reduced BA47 activity and Extraversion (p<0.05. Together, these results suggest common genetic variation associated with reduced FREM3 expression may confer risk for a subtype of depression characterized by reduced reactivity to environmental stimuli and slower perceptual processing speed, possibly suggestive of

  11. Aerodynamic Measurements of a Variable-Speed Power-Turbine Blade Section in a Transonic Turbine Cascade at Low Inlet Turbulence

    Science.gov (United States)

    Flegel-McVetta, Ashlie B.; Giel, Paul W.; Welch, Gerard E.

    2013-01-01

    Aerodynamic measurements obtained in a transonic linear cascade were used to assess the impact of large incidence angle and Reynolds number variations on the 3-D flow field and midspan loss and turning of a 2-D section of a variable-speed power-turbine (VSPT) rotor blade. Steady-state data were obtained for ten incidence angles ranging from +15.8 deg to -51.0 deg. At each angle, data were acquired at five flow conditions with the exit Reynolds number (based on axial chord) varying over an order-of-magnitude from 2.12×10(exp 5) to 2.12×10(exp 6). Data were obtained at the design exit Mach number of 0.72 and at a reduced exit Mach number of 0.35 as required to achieve the lowest Reynolds number. Midspan total-pressure and exit flow angle data were acquired using a five-hole pitch/yaw probe surveyed on a plane located 7.0 percent axial chord downstream of the blade trailing edge plane. The survey spanned three blade passages. Additionally, three-dimensional half-span flow fields were examined with additional probe survey data acquired at 26 span locations for two key incidence angles of +5.8 deg and -36.7 deg. Survey data near the endwall were acquired with a three-hole boundary-layer probe. The data were integrated to determine average exit total-pressure and flow angle as functions of incidence and flow conditions. The data set also includes blade static pressures measured on four spanwise planes and endwall static pressures. Tests were conducted in the NASA Glenn Transonic Turbine Blade Cascade Facility. The measurements reflect strong secondary flows associated with the high aerodynamic loading levels at large positive incidence angles and an increase in loss levels with decreasing Reynolds number. The secondary flows decrease with negative incidence as the blade becomes unloaded. Transitional flow is admitted in this low inlet turbulence dataset, making it a challenging CFD test case. The dataset will be used to advance understanding of the aerodynamic

  12. Optical dating of single sand-sized grains of quartz: Sources of variability

    DEFF Research Database (Denmark)

    Duller, G.A.T.; Bøtter-Jensen, L.; Murray, A.S.

    2000-01-01

    Optically stimulated luminescence (OSL) measurements have been made of over 3000 sand-sized grains of quartz. Analysis at this scale highlights the variability in the luminescence sensitivity and the dose saturation characteristics of individual quartz grains. Using a new instrument capable of me...... intensity, dose saturation characteristics and instrument uncertainty in equivalent dose calculation. (C) 2000 Elsevier Science Ltd. All rights reserved....

  13. Using Statistical Process Control Charts to Study Stuttering Frequency Variability during a Single Day

    Science.gov (United States)

    Karimi, Hamid; O'Brian, Sue; Onslow, Mark; Jones, Mark; Menzies, Ross; Packman, Ann

    2013-01-01

    Purpose: Stuttering varies between and within speaking situations. In this study, the authors used statistical process control charts with 10 case studies to investigate variability of stuttering frequency. Method: Participants were 10 adults who stutter. The authors counted the percentage of syllables stuttered (%SS) for segments of their speech…

  14. Modelling the variability of lag times and the first generation times of single cells of E. coli.

    Science.gov (United States)

    Métris, A; Le Marc, Y; Elfwing, A; Ballagi, A; Baranyi, J

    2005-04-15

    A mathematical model combining deterministic and stochastic elements describes the growth and division of single cells. Its deterministic part is based on the model of Baranyi and Roberts [International Journal of Food Microbiology 23 (1994) 277] modelling the gradual adjustment of the cells to a new environment. The stochastic part assumes a random threshold size for the division of a single cell, which accounts for the variability of the individual generation times. Experimental results of the first division times of thousands of single cells using a microscopic flow system could be reproduced with this model, and it has the potential to be used to study the effects of different stress and environmental factors on the distribution of the lag and generation times of individual cells.

  15. Inter-individual variability of oscillatory responses to subject's own name. A single-subject analysis.

    Science.gov (United States)

    Höller, Yvonne; Kronbichler, Martin; Bergmann, Jürgen; Crone, Julia Sophia; Schmid, Elisabeth Verena; Golaszewski, Stefan; Ladurner, Gunther

    2011-06-01

    In previous studies event-related potentials and oscillations in response to subject's own name have been analyzed extensively on group-level in healthy subjects and in patients with a disorder of consciousness. Subject's own name as a deviant produces a P3. With equiprobable stimuli, non-phase-locked alpha oscillations are smaller in response to subject's own name compared to other names or subject's own name backwards. However, little is known about replicability on a single-subject level. Seventeen healthy subjects were assessed in an own-name paradigm with equiprobable stimuli of subject's own name, another name, and subject's own name backwards. Event-related potentials and non-phase locked oscillations were analyzed with single-subject, non-parametric statistics. No consistent results were found either for ERPs or for the non-phase locked changes of oscillatory activities. Only 4 subjects showed a robust effect as expected, that is, a lower activity in the alpha-beta range to subject's own name compared to other conditions. Four subjects elicited a higher activity for subject's own name. Thus, analyzing the EEG reactivity in the own-name paradigm with equiprobable stimuli on a single-subject level yields a high variance between subjects. In future research, single-subject statistics should be applied for examining the validity of physiologic measurements in other paradigms and for examining the pattern of reactivity in patients. Copyright © 2011 Elsevier B.V. All rights reserved.

  16. Production Variability and Single Word Intelligibility in Aphasia and Apraxia of Speech

    Science.gov (United States)

    Haley, Katarina L.; Martin, Gwenyth

    2011-01-01

    This study was designed to estimate test-retest reliability of orthographic speech intelligibility testing in speakers with aphasia and AOS and to examine its relationship to the consistency of speaker and listener responses. Monosyllabic single word speech samples were recorded from 13 speakers with coexisting aphasia and AOS. These words were…

  17. Variability of writing disorders in Wernicke's aphasia underperforming different writing tasks: A single-case study.

    Science.gov (United States)

    Kozintseva, Elena; Skvortsov, Anatoliy

    2016-03-01

    The aim of our study was to evolve views on writing disorders in Wernicke's agraphia by comparing group data and analysis of a single patient. We showed how a single-case study can be useful in obtaining essential results that can be hidden by averaging group data. Analysis of a single patient proved to be important for resolving contradictions of the "holistic" and "elementaristic" paradigms of psychology and for the development of theoretical knowledge with the example of a writing disorder. The implementation of a holistic approach was undertaken by presenting the tasks differing in functions in which writing had been performed since its appearance in human culture (communicative, mnestic, and regulatory). In spite of the identical composition of involved psychological components, these differences were identified when certain types of errors were analyzed in the single subject. The results are discussed in terms of used writing strategy, resulting in a way of operation of involved components that lead to qualitative and quantitative changes of writing errors within the syndrome of Wernicke's agraphia. © 2016 The Institute of Psychology, Chinese Academy of Sciences and John Wiley & Sons Australia, Ltd.

  18. Sources of variability in OSL dose measurements using single grains of quartz

    DEFF Research Database (Denmark)

    Thomsen, Kristina Jørkov; Murray, A.S.; Bøtter-Jensen, L.

    2005-01-01

    spread. In this preliminary study, dose distributions have been studied using single grains of heated and laboratory irradiated quartz. By heating the sample, the contribution from incomplete zeroing was excluded and at the same time the sample was sensitised. The laboratory gamma irradiation...

  19. High-fidelity teleportation of continuous-variable quantum States using delocalized single photons

    DEFF Research Database (Denmark)

    Andersen, Ulrik L; Ralph, Timothy C

    2013-01-01

    states as in traditional teleportation but on an ensemble of single photon entangled states. We characterize the teleportation scheme with coherent states, mesoscopic superposition states, and two-mode squeezed states and we find several situations in which near-unity teleportation fidelity can...

  20. Repetition rates of specific interval patterns in single spike train reflect excitation level of specific receptor types, shown by high-speed favored-pattern detection method.

    Science.gov (United States)

    Ku, Yun-Hui; Wang, Ming; Li, Yao-Hua; Sun, Ze-Jin; Guo, Tao; Wu, Jin-Sheng

    2006-10-03

    Interval patterns in single spike train, e.g. "favored patterns (FPs, the FP is a sequence of successive intervals of action potentials that occur more often than what is reasonably expected at random.)", may represent neural codes containing information. The present study developed a "high-speed FP-detection method" which could qualitatively and quantitatively analyze FPs. By using this method, single spike trains of nucleus paraventricularis (NPV) and rostral ventrolateral medulla (RVL) having different firing patterns, being involved in regulation of arterial pressure, and controlled by different transmitters, were chosen for analysis. (1) Corticotropin releasing factor, substance P and agonists of alpha-, beta- and M-receptor microinjected into these brain areas, respectively, induced dominant change of specific FP. Repetition rates of specific FPs reflect excitation level of specific receptor types. It shows that chemical codes (different transmitters with their receptor types or subtypes) are transformed into electrical codes (different FPs). (2) When alpha-, beta- and M-receptors of RVL neurons were activated simultaneously by intrinsic excitatory transmitters released due to activation of input pathway, only repetition rate of the specific FP that represented the predominant activity of the receptor type (alpha-adrenergic receptor) markedly increased. The activities of other receptor types (beta- and M-receptors) were masked. (3) Intrinsic inhibitory transmitters (GABA, beta-endorphin) in the RVL all decreased specific FP repetition rate of dominant receptor type. These results may provide a new way to further explore how information in the CNS is conveyed and processed.

  1. Efficient noise-tolerant estimation of heart rate variability using single-channel photoplethysmography.

    Science.gov (United States)

    Firoozabadi, Reza; Helfenbein, Eric D; Babaeizadeh, Saeed

    2017-08-18

    The feasibility of using photoplethysmography (PPG) for estimating heart rate variability (HRV) has been the subject of many recent studies with contradicting results. Accurate measurement of cardiac cycles is more challenging in PPG than ECG due to its inherent characteristics. We developed a PPG-only algorithm by computing a robust set of medians of the interbeat intervals between adjacent peaks, upslopes, and troughs. Abnormal intervals are detected and excluded by applying our criteria. We tested our algorithm on a large database from high-risk ICU patients containing arrhythmias and significant amounts of artifact. The average difference between PPG-based and ECG-based parameters is SDSD and RMSSD. Our performance testing shows that the pulse rate variability (PRV) parameters are comparable to the HRV parameters from simultaneous ECG recordings. Copyright © 2017 Elsevier Inc. All rights reserved.

  2. Functional groups in a single pteridosperm species: Variability and circumscription (Pennsylvanian, Nova Scotia, Canada)

    Energy Technology Data Exchange (ETDEWEB)

    Mastalerz, Maria [Indiana Geological Survey, Indiana University, 611 North Walnut Grove, Bloomington, IN 47405-2208 (United States); Zodrow, Erwin L.

    2007-05-01

    Multiple foliar specimens of the Late Pennsylvanian fossil pteridosperm [gymnosperm] Alethopteris zeilleri (Ragot) Wagner were collected from one restricted stratigraphical horizon in the Canadian Sydney Coalfield. Variability of functional-group distribution using FTIR technique was studied in compressions, adaxial versus abaxial cuticles, and in unseparated cuticles as a function of maceration time from 48 to 168 h. The results obtained document spectral variability that could be expected within specimens of one species. For example, CH{sub 2}/CH{sub 3} and Al/ox ratios can differ by as much as 20% of the values. Moreover, the experiments performed confirm that by using a previously established maceration protocol, long maceration periods do not bias FTIR spectra in terms of oxygenation overprinting. The inference that this cuticle is robust, under the given diagenetic level, probably reflects a reassuring degree of chemical fidelity of the Pennsylvanian plant to support Carboniferous chemotaxonomic observations. (author)

  3. HiPERCAM: a high-speed quintuple-beam CCD camera for the study of rapid variability in the universe

    Science.gov (United States)

    Dhillon, Vikram S.; Marsh, Thomas R.; Bezawada, Naidu; Black, Martin; Dixon, Simon; Gamble, Trevor; Henry, David; Kerry, Paul; Littlefair, Stuart; Lunney, David W.; Morris, Timothy; Osborn, James; Wilson, Richard W.

    2016-08-01

    HiPERCAM is a high-speed camera for the study of rapid variability in the Universe. The project is funded by a ɛ3.5M European Research Council Advanced Grant. HiPERCAM builds on the success of our previous instrument, ULTRACAM, with very significant improvements in performance thanks to the use of the latest technologies. HiPERCAM will use 4 dichroic beamsplitters to image simultaneously in 5 optical channels covering the u'g'r'I'z' bands. Frame rates of over 1000 per second will be achievable using an ESO CCD controller (NGC), with every frame GPS timestamped. The detectors are custom-made, frame-transfer CCDs from e2v, with 4 low noise (2.5e-) outputs, mounted in small thermoelectrically-cooled heads operated at 180 K, resulting in virtually no dark current. The two reddest CCDs will be deep-depletion devices with anti-etaloning, providing high quantum efficiencies across the red part of the spectrum with no fringing. The instrument will also incorporate scintillation noise correction via the conjugate-plane photometry technique. The opto-mechanical chassis will make use of additive manufacturing techniques in metal to make a light-weight, rigid and temperature-invariant structure. First light is expected on the 4.2m William Herschel Telescope on La Palma in 2017 (on which the field of view will be 10' with a 0.3"/pixel scale), with subsequent use planned on the 10.4m Gran Telescopio Canarias on La Palma (on which the field of view will be 4' with a 0.11"/pixel scale) and the 3.5m New Technology Telescope in Chile.

  4. A novel variable antibody fragment dimerized by leucine zippers with enhanced neutralizing potency against rabies virus G protein compared to its corresponding single-chain variable antibody fragment.

    Science.gov (United States)

    Li, Zhuang; Cheng, Yue; Xi, Hualong; Gu, Tiejun; Yuan, Ruosen; Chen, Xiaoxu; Jiang, Chunlai; Kong, Wei; Wu, Yongge

    2015-12-01

    Fatal rabies can be prevented effectively by post-exposure prophylactic (PEP) with rabies immunoglobulin (RIG). Single-chain variable fragments (scFv), which are composed of a variable heavy chain (VH) and a variable light chain (VL) connected by a peptide linker, can potentially be used to replace RIG. However, in our previous study, a scFv (scFV57S) specific for the rabies virus (RV) G protein showed a lower neutralizing potency than that of its parent IgG due to lower stability and altered peptide assembly pattern. In monoclonal antibodies, the VH and VL interact non-covalently, while in scFvs the VH is connected covalently with the VL by the artificial linker. In this study, we constructed and expressed two peptides 57VL-JUN-HIS and 57VH-FOS-HA in Escherichia coli. The well-known Fos and Jun leucine zippers were utilized to dimerize VH and VL similarly to the IgG counterpart. The two peptides assembled to form zipFv57S in vitro. Due to the greater similarity in structure with IgG, the zipFv57S protein showed a higher binding ability and affinity resulting in notable improvement of in vitro neutralizing activity over its corresponding scFv. The zipFv57S protein was also found to be more stable and showed similar protective rate as RIG in mice challenged with a lethal dose of RV. Our results not only indicated zipFv57S as an ideal alternative for RIG in PEP but also offered a novel and efficient hetero-dimerization pattern of VH and VL leading to enhanced neutralizing potency. Copyright © 2015. Published by Elsevier Ltd.

  5. Anesthesia effect on single local field potentials variability in rat barrel cortex: Preliminary results.

    Science.gov (United States)

    Cecchetto, Claudia; Mahmud, Mufti; Vassanelli, Stefano

    2015-01-01

    The rat barrel cortex is a widely used model of information processing in the somatosensory area, thanks to its precise and easily recognizable organization. However, evoked Local Field Potentials (LFPs) generated in the barrel cortex by repetitive deflections of rat whiskers show large variability in shapes and timings. Moreover, anesthetics can deeply affect the profile of evoked responses. This paper presents preliminary report on the variability and the effect of commonly used anesthetics on these signals. We studied representative signal shape characteristics (e.g., latency and amplitude of events) extracted from evoked responses acquired by means of standard Ag/AgCl electrodes from different cortical layers. As an early result, we found significant difference in the latency of the first principal peak of the responses. Under Tiletamine-Xylazine anesthetic, the responses or events of the evoked LFPs occurred later than the ones recorded while urethane was administered. Furthermore, the distributions of the peak latencies in all cortical layers were narrower in case of Urethane. This behavior should be attributed to the different effects of these two anesthetics on specific synaptic receptors and thus on the processing of neural information and the encoding of sensory input along the cortical pathway.

  6. Variable contact gap single-molecule conductance determination for a series of conjugated molecular bridges

    DEFF Research Database (Denmark)

    Haiss, W.; Wang, Christian; Jitchati, R.

    2008-01-01

    It is now becoming clear that the characteristics of the whole junction are important in determining the conductance of single molecules bound between two metal contacts. This paper shows through measurements on a series of seven conjugated molecular bridges that contact separation is an importan...... that conductance increases rather dramatically at higher tilt angle away from the normal for conformationally rigid molecular wires and that this increase in conductance arises from increased electronic coupling between the molecular bridge and the gold contacts.......It is now becoming clear that the characteristics of the whole junction are important in determining the conductance of single molecules bound between two metal contacts. This paper shows through measurements on a series of seven conjugated molecular bridges that contact separation is an important......-distance curves and knowledge of the terminal to terminal length of the molecular wire. The contact gap separation dependence is interpreted as arising from tilting of these molecules in the junction and this model is underpinned by ab initio transport computations. In this respect we make the general observation...

  7. Simultaneous effects of single wall carbon nanotube and effective variable viscosity for peristaltic flow through annulus having permeable walls

    Directory of Open Access Journals (Sweden)

    Iqra Shahzadi

    Full Text Available The current article deals with the combine effects of single wall carbon nanotubes and effective viscosity for the peristaltic flow of nanofluid through annulus. The nature of the walls is assumed to be permeable. The present theoretical model can be considered as mathematical representation to the motion of conductive physiological fluids in the existence of the endoscope tube which has many biomedical applications such as drug delivery system. The outer tube has a wave of sinusoidal nature that is travelling along its walls while the inner tube is rigid and uniform. Lubrication approach is used for the considered analysis. An empirical relation for the effective variable viscosity of nanofluid is proposed here interestingly. The viscosity of nanofluid is the function of radial distance and the concentration of nanoparticles. Exact solution for the resulting system of equations is displayed for various quantities of interest. The outcomes show that the maximum velocity of SWCNT-blood nanofluid enhances for larger values of viscosity parameter. The pressure gradient in the more extensive part of the annulus is likewise found to increase as a function of variable viscosity parameter. The size of the trapped bolus is also influenced by variable viscosity parameter. The present examination also revealed that the carbon nanotubes have many applications related to biomedicine. Keywords: Variable nanofluid viscosity, SWCNT, Annulus, Permeable walls, Exact solution

  8. Reducing the risk of rear-end collisions with infrastructure-to-vehicle (I2V) integration of variable speed limit control and adaptive cruise control system.

    Science.gov (United States)

    Li, Ye; Wang, Hao; Wang, Wei; Liu, Shanwen; Xiang, Yun

    2016-08-17

    Adaptive cruise control (ACC) has been investigated recently to explore ways to increase traffic capacity, stabilize traffic flow, and improve traffic safety. However, researchers seldom have studied the integration of ACC and roadside control methods such as the variable speed limit (VSL) to improve safety. The primary objective of this study was to develop an infrastructure-to-vehicle (I2V) integrated system that incorporated both ACC and VSL to reduce rear-end collision risks on freeways. The intelligent driver model was firstly modified to simulate ACC behavior and then the VSL strategy used in this article was introduced. Next, the I2V system was proposed to integrate the 2 advanced techniques, ACC and VSL. Four scenarios of no control, VSL only, ACC only, and the I2V system were tested in simulation experiments. Time exposed time to collision (TET) and time integrated time to collision (TIT), 2 surrogate safety measures derived from time to collision (TTC), were used to evaluate safety issues associated with rear-end collisions. The total travel times of each scenario were also compared. The simulation results indicated that both the VSL-only and ACC-only methods had a positive impact on reducing the TET and TIT values (reduced by 53.0 and 58.6% and 59.0 and 65.3%, respectively). The I2V system combined the advantages of both ACC and VSL to achieve the most safety benefits (reduced by 71.5 and 77.3%, respectively). Sensitivity analysis of the TTC threshold also showed that the I2V system obtained the largest safety benefits with all of the TTC threshold values. The impact of different market penetration rates of ACC vehicles in I2V system indicated that safety benefits increase with an increase in ACC proportions. Compared to VSL-only and ACC-only scenarios, this integrated I2V system is more effective in reducing rear-end collision risks. The findings of this study provide useful information for traffic agencies to implement novel techniques to improve

  9. Single genome retrieval of context-dependent variability in mutation rates for human germline.

    Science.gov (United States)

    Sahakyan, Aleksandr B; Balasubramanian, Shankar

    2017-01-13

    Accurate knowledge of the core components of substitution rates is of vital importance to understand genome evolution and dynamics. By performing a single-genome and direct analysis of 39,894 retrotransposon remnants, we reveal sequence context-dependent germline nucleotide substitution rates for the human genome. The rates are characterised through rate constants in a time-domain, and are made available through a dedicated program (Trek) and a stand-alone database. Due to the nature of the method design and the imposed stringency criteria, we expect our rate constants to be good estimates for the rates of spontaneous mutations. Benefiting from such data, we study the short-range nucleotide (up to 7-mer) organisation and the germline basal substitution propensity (BSP) profile of the human genome; characterise novel, CpG-independent, substitution prone and resistant motifs; confirm a decreased tendency of moieties with low BSP to undergo somatic mutations in a number of cancer types; and, produce a Trek-based estimate of the overall mutation rate in human. The extended set of rate constants we report may enrich our resources and help advance our understanding of genome dynamics and evolution, with possible implications for the role of spontaneous mutations in the emergence of pathological genotypes and neutral evolution of proteomes.

  10. SELF-ALIGNED SINGLE CRYSTAL CONTACTED HIGH-SPEED SILICON BIPOLAR TRANSISTOR UTILIZING SELECTIVE (SEG) AND CONFINED SELECTIVE EPITAXIAL GROWTH (CLSEG)

    OpenAIRE

    Siekkinen, James W.; Neudeck, Gerold W.

    1992-01-01

    A new high-speed bipolar transistor structure, the ELOBJT-3, is proposed as a novel application of selective epitaxy technology. The new structure is greatly suited to high-speed ECL circuits, where Ccb, C,, and Rbx are of prime importance. The reduction of these parasitics to their nearly theoretical minimums is accomplished through the use of dielectric isolation and concentric contacting. For extremely high speed operation, dimensions can be scaled to sub-micron size due to the completely ...

  11. Individual variability in response to a single sickling event for normal, sickle cell, and sickle trait erythrocytes.

    Science.gov (United States)

    Tarasev, Michael; Muchnik, Marina; Light, Lucia; Alfano, Kenneth; Chakraborty, Sumita

    2017-03-01

    Hemoglobin S (Hb-S) polymerization is the primary event in sickle cell disease causing irreversible damage to red blood cell (RBC) membranes over repeated polymerization cycles. A single polymerization triggered by a hypoxic environment was reported to result in reversibly (upon reoxygenation) decreased RBC deformability and increased mechanical fragility (MF). Individualized responses have not been reported, although RBC fragility can vary significantly even among healthy individuals. This study evaluates individual variability in response to a single hypoxia-induced sickling event, through changes in RBC MF. Blood was drawn from 10 normal (AA), 11 sickle cell (SS), and 7 sickle trait (AS) subjects-with Hb-S fraction, osmotic fragility, and medical history also collected. Mechanical stress was applied using a bead mill at 50-Hz oscillation for 0.5-30 minutes. MF profiles here give percent hemolysis upon successive durations of stressing. MF was measured for AA, SS, and AS cells-each equilibrated (1) with air, (2) with nitrogen in an anaerobic chamber, and (3) with air after the hypoxic event. While AA subjects exhibited significantly different changes in fragility upon hypoxia, in all cases there was recovery to close to the initial MF values on reoxygenation. For AS subjects, recovery at reoxygenation was observed only in about half of the cases. Fragility of SS cells increased in hypoxia and decreased with reoxygenation, with significantly variable magnitude of recovery. The variability of response for individual AS and SS subjects indicates that some are potentially at higher risk of irreversible hypoxia-induced membrane damage. Copyright © 2016 Elsevier Inc. All rights reserved.

  12. Performance analysis of a novel planetary speed increaser used in single-rotor wind turbines with counter-rotating electric generator

    Science.gov (United States)

    Saulescu, R.; Neagoe, M.; Munteanu, O.; Cretescu, N.

    2016-08-01

    The paper presents a study on the kinematic and static performances of a new type of 1DOF (Degree Of Freedom) planetary speed increaser to be implemented in wind turbines, a transmission with three operating cases: a) one input and one output, b) one input and two outputs, in which the speed of the secondary output is equal to the input speed, and c) with one input and two outputs, where the secondary output speed is higher than the input speed. The proposed speed increaser contains two sun gears and a double satellite, allowing operation with an output connected to the fixed stator of a classic generator (case I) or with two counterrotating outputs that drive a counter-rotating generator (with a mobile stator). A new variant of planetary transmission capable of providing the speed increase of the generator stator and, thus, the increase of the relative speed between the generator rotor and stator is obtained by the parallel connection of the speed increaser with a planetary gear. The three conceptual variants of planetary transmission are analytically modelled and comparatively analysed based on a set of kinematic and static parameters. The proposed transmission has higher performances compared to the same transmission with one input and one output, the increase of the kinematic amplification ratio and efficiency being achieved simultaneously.

  13. Population-Level Neural Codes Are Robust to Single-Neuron Variability from a Multidimensional Coding Perspective.

    Science.gov (United States)

    Montijn, Jorrit S; Meijer, Guido T; Lansink, Carien S; Pennartz, Cyriel M A

    2016-08-30

    Sensory neurons are often tuned to particular stimulus features, but their responses to repeated presentation of the same stimulus can vary over subsequent trials. This presents a problem for understanding the functioning of the brain, because downstream neuronal populations ought to construct accurate stimulus representations, even upon singular exposure. To study how trial-by-trial fluctuations (i.e., noise) in activity influence cortical representations of sensory input, we performed chronic calcium imaging of GCaMP6-expressing populations in mouse V1. We observed that high-dimensional response correlations, i.e., dependencies in activation strength among multiple neurons, can be used to predict single-trial, single-neuron noise. These multidimensional correlations are structured such that variability in the response of single neurons is relatively harmless to population representations of visual stimuli. We propose that multidimensional coding may represent a canonical principle of cortical circuits, explaining why the apparent noisiness of neuronal responses is compatible with accurate neural representations of stimulus features. Copyright © 2016 The Author(s). Published by Elsevier Inc. All rights reserved.

  14. SINGLE CHAIN VARIABLE FRAGMENTS OF ANTIBODIES AGAINST DIPHTHERIA TOXIN B-SUBUNIT ISOLATED FROM PHAGE DISPLAY HUMAN ANTIBODY LIBRARY

    Directory of Open Access Journals (Sweden)

    Oliinyk O. S.

    2014-02-01

    Full Text Available Diphtheria toxin is an exoantigen of Corynebacterium diphtheriae that inhibits protein synthesis and kills sensitive cells. The aim of this study was to obtain human recombinant single-chain variable fragment (scFv antibodies against receptor-binding B subunit of diphtheria toxin. 12 specific clones were selected after three rounds of a phage display naїve (unimmunized human antibody library against recombinant B-subunit. scFv DNA inserts from these 12 clones were digested with MvaI, and 6 unique restriction patterns were found. Single-chain antibodies were expressed in Escherichia coli XL1-blue. The recombinant proteins were characterized by immunoblotting of bacterial extracts and detection with an anti-E-tag antibody. The toxin B-subunit-binding function of the single-chain antibody was shown by ELISA. The affinity constants for different clones were found to be from 106 to 108 М–1. Due to the fact, that these antibody fragments recognized epitopes in the receptor-binding Bsubunit of diphtheria toxin, further studies are interesting to evaluate their toxin neutralization properties and potential for therapeutic applications. Obtained scFv-antibodies can also be used for detection and investigation of biological properties of diphtheria toxin.

  15. Population-Level Neural Codes Are Robust to Single-Neuron Variability from a Multidimensional Coding Perspective

    Directory of Open Access Journals (Sweden)

    Jorrit S. Montijn

    2016-08-01

    Full Text Available Sensory neurons are often tuned to particular stimulus features, but their responses to repeated presentation of the same stimulus can vary over subsequent trials. This presents a problem for understanding the functioning of the brain, because downstream neuronal populations ought to construct accurate stimulus representations, even upon singular exposure. To study how trial-by-trial fluctuations (i.e., noise in activity influence cortical representations of sensory input, we performed chronic calcium imaging of GCaMP6-expressing populations in mouse V1. We observed that high-dimensional response correlations, i.e., dependencies in activation strength among multiple neurons, can be used to predict single-trial, single-neuron noise. These multidimensional correlations are structured such that variability in the response of single neurons is relatively harmless to population representations of visual stimuli. We propose that multidimensional coding may represent a canonical principle of cortical circuits, explaining why the apparent noisiness of neuronal responses is compatible with accurate neural representations of stimulus features.

  16. Comprehensive Performance Evaluation of a High Speed Brushless DC Motor Using an Improved Phase Variable Model(Special Issue to the Asia-Pacific Symposium on Applied Electromagnetics and Mechanics (APSAEM06))

    OpenAIRE

    Jiaxin, Chen; Youguang, Guo; Jianguo, Zhu; College of Electromechanical Engineering, Donghua University : Faculty of Engineering, University of Technology; Faculty of Engineering, University of Technology; Faculty of Engineering, University of Technology

    2007-01-01

    This paper presents the performance evaluation of a high-speed surface mounted PM brushless DC motor by using an improved phase variable model. Magnetic field finite element analyses are conducted to accurately calculate the key motor parameters such as air gap flux, back electromotive force and inductance, and their dependence on rotor position and magnetic saturation. Based on the numerical magnetic field solutions, a modified incremental energy method is applied to effectively calculate th...

  17. Effect of water injection and off scheduling of variable inlet guide vanes, gas generator speed and power turbine nozzle angle on the performance of an automotive gas turbine engine

    Science.gov (United States)

    Warren, E. L.

    1980-01-01

    The Chrysler/ERDA baseline automotive gas turbine engine was used to experimentally determine the power augmentation and emissions reductions achieved by the effect of variable compressor and power engine geometry, water injection downstream of the compressor, and increases in gas generator speed. Results were dependent on the mode of variable geometry utilization. Over 20 percent increase in power was accompanied by over 5 percent reduction in SFC. A fuel economy improvement of at least 6 percent was estimated for a vehicle with a 75 kW (100 hp) engine which could be augmented to 89 kW (120 hp) relative to an 89 Kw (120 hp) unaugmented engine.

  18. Wind-Tunnel and Flight Test Results for the Measurements of Flow Variables at Supersonic Speeds Using Improved Wedge and Conical Probes

    Science.gov (United States)

    Bobbitt, Percy J.; Maglieri, Domenic J.; Banks, Daniel W.; Frederick, Michael A.; Fuchs, Aaron W.

    2012-01-01

    The results of supersonic wind-tunnel tests on three probes at nominal Mach numbers of 1.6, 1.8 and 2.0 and flight tests on two of these probes up to a Mach number of 1.9 are described. One probe is an 8 deg. half-angle wedge with two total-pressure measurements and one static. The second, a conical probe, is a cylinder that has a 15 deg., semi-angle cone tip with one total-pressure orifice at the apex and four static-pressure orifices on the surface of the cone, 90 deg. apart, and about two-thirds of the distance from the cone apex to the base of the cone. The third is a 2 deg. semi-angle cone that has two static ports located 180 deg. apart about 1.5 inches behind the apex of the cone. The latter probe was included since it has been the "probe of choice" for wind-tunnel flow-field pressure measurements (or one similar to it) for the past half-century. The wedge and 15 deg. conical probes used in these tests were designed for flight diagnostic measurements for flight Mach numbers down to 1.35 and 1.15 respectively, and have improved capabilities over earlier probes of similar shape. The 15. conical probe also has a temperature sensor that is located inside the cylindrical part of the probe that is exposed to free-stream flow through an annulus at the apex of the cone. It enables the determination of free-stream temperature, density, speed of sound, and velocity, in addition to free-stream pressure, Mach number, angle of attack and angle of sideslip. With the time-varying velocity, acceleration can be calculated. Wind-tunnel tests of the two probes were made in NASA Langley Research Center fs Unitary Plan Wind Tunnel (UPWT) at Mach numbers of 1.6, 1.8, and 2.0. Flight tests were carried out at the NASA Dryden Flight Research Center (DFRC) on its F-15B aircraft up to Mach numbers of 1.9. The probes were attached to a fixture, referred to as the Centerline Instrumented Pylon (CLIP), under the fuselage of the aircraft. Problems controlling the velocity of the flow

  19. Development of anti-CD47 single-chain variable fragment targeted magnetic nanoparticles for treatment of human bladder cancer.

    Science.gov (United States)

    Rezaei, Gashin; Habibi-Anbouhi, Mahdi; Mahmoudi, Morteza; Azadmanesh, Kayhan; Moradi-Kalbolandi, Shima; Behdani, Mahdi; Ghazizadeh, Leila; Abolhassani, Mohsen; Shokrgozar, Mohammad Ali

    2017-03-01

    To develop a novel anti-CD47 single-chain variable fragment (scFv) functionalized magnetic nanoparticles (MNPs) for targeting bladder cell lines and its applicability in thermotherapy. An immunized murine antibody phage display library was constructed and screened to isolate anti-CD47 binders. A scFv was selected and conjugated to MNPs which was then utilized to discriminate CD47+ bladder cells along with assessing its efficacy in thermotherapy. An scFv with high affinity to bladder cells was efficiently conjugated to MNPs. Following a hyperthermia treatment, the function of scFv-MNP conjugates led to a considerable reduction in cell viability. The anti-CD47 scFv-MNP conjugate was an effective cancer cell thermotherapy tool that might pave the way for development of bionano-based targeting techniques in both early detection and treatment of cancer.

  20. Construction of Recombinant Single Chain Variable Fragment (ScFv) Antibody Against Superantigen for Immunodetection Using Antibody Phage Display Technology.

    Science.gov (United States)

    Singh, Pawan Kumar; Agrawal, Ranu; Kamboj, D V; Singh, Lokendra

    2016-01-01

    Superantigens are a class of antigens that bind to the major histocompatibility complex class (MHC) II and T-cell receptor (TCR) and cause the nonspecific activation of T cells, resulting in a massive release of pro-inflammatory mediators. They are produced by the gram-positive organisms Staphylococcus aureus and Streptococcus pyogenes, and by a variety of other microbes such as viruses and mycoplasma, and cause toxic shock syndrome (TSS) and even death in some cases. The immunodetection of superantigens is difficult due to the polyclonal activation of T-cells leading to nonspecific antibody production. The production of recombinant monoclonal antibodies against superantigens can solve this problem and are far better than polyclonal antibodies in terms of detection. Here, we describe the construction of recombinant single chain variable fragments (ScFv) antibodies against superantigens with specific reference to SEB (staphylococcal enterotoxin B) using antibody phage display technology.

  1. Large Intra-subject Variability in Caffeine Pharmacokinetics: Randomized Cross-over Study of Single Caffeine Product.

    Science.gov (United States)

    Hammami, Muhammad M; Alvi, Syed N

    2017-09-01

    Background Average bioequivalence has been criticized for not adequately addressing individual variations. Importance of subjects' blinding in bioequivalence studies has not been well studied. We explored the extent of intra-subject pharmacokinetic variability and effect of drug-ingestion unawareness in subjects taking single caffeine product. Methods A single-dose randomized cross-over design was used to compare pharmacokinetics of 200 mg caffeine, described as caffeine (overt) or as placebo (covert). Maximum concentration (Cmax), Cmax first time (Tmax), area-under-the-concentration-time-curve, to last measured concentration (AUCT), extrapolated to infinity (AUCI), or to Tmax of overt caffeine (AUCOverttmax), and Cmax/AUCI were calculated blindly using standard non-compartmental method. Percentages of individual covert/overt ratios that are outside the ±25% range were determined. Covert-vs-overt effect on caffeine pharmacokinetics was evaluated by 90% confidence interval (CI) and 80.00-125.00% bioequivalence range. Results 32 healthy subjects (6% females, mean (SD) age 33.3 (7.2) year) participated in the study (28 analysed). Out of the 28 individual covert/overt ratios, 23% were outside the ±25% range for AUCT, 30% for AUCI, 20% for AUCOverttmax, 30% for Cmax, and 43% for Tmax. There was no significant covert-vs-overt difference in any of the pharmacokinetic parameters studied. Further, the 90% CIs for AUCT, AUCI, Cmax, AUCOverttmax, and Cmax/AUCI were all within the 80.00-125.00% bioequivalence range with mean absolute deviation of covert/overt ratios of 3.31%, 6.29%, 1.43%, 1.87%, and 5.19%, respectively. Conclusions Large intra-subject variability in main caffeine pharmacokinetic parameters was noted when comparing an oral caffeine product to itself. Subjects' blinding may not be important in average bioequivalence studies. © Georg Thieme Verlag KG Stuttgart · New York.

  2. A single-chain variable fragment intrabody prevents intracellular polymerization of Z α1-antitrypsin while allowing its antiproteinase activity

    Science.gov (United States)

    Ordóñez, Adriana; Pérez, Juan; Tan, Lu; Dickens, Jennifer A.; Motamedi-Shad, Neda; Irving, James A.; Haq, Imran; Ekeowa, Ugo; Marciniak, Stefan J.; Miranda, Elena; Lomas, David A.

    2015-01-01

    Mutant Z α1-antitrypsin (E342K) accumulates as polymers within the endoplasmic reticulum (ER) of hepatocytes predisposing to liver disease, whereas low levels of circulating Z α1-antitrypsin lead to emphysema by loss of inhibition of neutrophil elastase. The ideal therapy should prevent polymer formation while preserving inhibitory activity. Here we used mAb technology to identify interactors with Z α1-antitrypsin that comply with both requirements. We report the generation of an mAb (4B12) that blocked α1-antitrypsin polymerization in vitro at a 1:1 molar ratio, causing a small increase of the stoichiometry of inhibition for neutrophil elastase. A single-chain variable fragment (scFv) intrabody was generated based on the sequence of mAb4B12. The expression of scFv4B12 within the ER (scFv4B12KDEL) and along the secretory pathway (scFv4B12) reduced the intracellular polymerization of Z α1-antitrypsin by 60%. The scFv4B12 intrabody also increased the secretion of Z α1-antitrypsin that retained inhibitory activity against neutrophil elastase. MAb4B12 recognized a discontinuous epitope probably located in the region of helices A/C/G/H/I and seems to act by altering protein dynamics rather than binding preferentially to the native state. This novel approach could reveal new target sites for small-molecule intervention that may block the transition to aberrant polymers without compromising the inhibitory activity of Z α1-antitrypsin.—Ordóñez, A., Pérez, J., Tan, L., Dickens, J. A., Motamedi-Shad, N., Irving, J. A., Haq, I., Ekeowa, U., Marciniak, S. J., Miranda, E., Lomas, D. A. A single-chain variable fragment intrabody prevents intracellular polymerization of Z α1-antitrypsin while allowing its antiproteinase activity. PMID:25757566

  3. Periodic population codes: From a single circular variable to higher dimensions, multiple nested scales, and conceptual spaces.

    Science.gov (United States)

    Herz, Andreas Vm; Mathis, Alexander; Stemmler, Martin

    2017-10-01

    Across the nervous system, neurons often encode circular stimuli using tuning curves that are not sine or cosine functions, but that belong to the richer class of von Mises functions, which are periodic variants of Gaussians. For a population of neurons encoding a single circular variable with such canonical tuning curves, computing a simple population vector is the optimal read-out of the most likely stimulus. We argue that the advantages of population vector read-outs are so compelling that even the neural representation of the outside world's flat Euclidean geometry is curled up into a torus (a circle times a circle), creating the hexagonal activity patterns of mammalian grid cells. Here, the circular scale is not set a priori, so the nervous system can use multiple scales and gain fields to overcome the ambiguity inherent in periodic representations of linear variables. We review the experimental evidence for this framework and discuss its testable predictions and generalizations to more abstract grid-like neural representations. Copyright © 2017 Elsevier Ltd. All rights reserved.

  4. Development and validation of a single-tube multiple-locus variable number tandem repeat analysis for Klebsiella pneumoniae.

    Directory of Open Access Journals (Sweden)

    Antoinette A T P Brink

    Full Text Available Genotyping of Klebsiella pneumoniae is indispensable for management of nosocomial infections, monitoring of emerging strains--including extended-spectrum beta-lactamase (ESBL producers-, and general epidemiology. Such objectives require a high-resolution genotyping method with a fixed scheme that allows (1 long-term retrospective and prospective assessment, (2 objective result readout and (3 library storage for database development and exchangeable results. We have developed a multiple-locus variable number tandem repeat analysis (MLVA using a single-tube fluorescently primed multiplex PCR for 8 Variable Number Tandem Repeats (VNTRs and automated fragment size analysis. The type allocation scheme was optimized using 224 K. pneumoniae clinical isolates, which yielded 101 MLVA types. The method was compared to the gold standard multilocus sequence typing (MLST using a subset of these clinical isolates (n = 95 and found to be highly concordant, with at least as high a resolution but with considerably less hands-on time. Our results position this MLVA scheme as an appropriate, high-throughput and relatively low-cost tool for K. pneumoniae epidemiology.

  5. The effect of varying the peptide linker length in a single chain variable fragment antibody against wogonin glucuronide.

    Science.gov (United States)

    Paudel, Madan Kumar; Sakamoto, Seiichi; Van Huy, Le; Tanaka, Hiroyuki; Miyamoto, Tomofumi; Morimoto, Satoshi

    2017-06-10

    Peptide linkers of three different lengths were constructed to join the variable regions of the heavy chain (VH) and the light chain (VL) in a single-chain variable fragment antibody (scFv) specific for wogonin glucuronide (Wgn) that has the structure VH-(GGGGS) n -VL (n=3, 5, or 7). The scFv antibodies, which were expressed in Escherichia coli, were derived from an anti-Wgn monoclonal antibody (315A). An indirect competitive enzyme-linked immunosorbent assay (icELISA) was used to evaluate their reactivity and sensitivity, which is also used for quantitative analysis of Wgn. Our results, showed that the reactivity and specificity of the three different scFvs were, in fact, similar. Subsequently, the scFv having a VH-(GGGGS) 3 -VL linker which was slightly better that other two scFvs against Wgn, was applied to indirect competitive ELISA (icELISA) to analyze Scutellariae Radix (S. Radix). The utility of the icELISA was demonstrated for quality control and analysis of S. Radix in this report. Copyright © 2017 Elsevier B.V. All rights reserved.

  6. Anti-oligomeric Abeta single-chain variable domain antibody blocks Abeta-induced toxicity against human neuroblastoma cells.

    Science.gov (United States)

    Zameer, Andleeb; Kasturirangan, Srinath; Emadi, Sharareh; Nimmagadda, Sridevi V; Sierks, Michael R

    2008-12-26

    The Amyloid-beta (Abeta) peptide is a major component of the amyloid plaques associated with Alzheimer's disease (AD). Recent studies suggest that the most toxic forms of Abeta are small, soluble oligomeric aggregates. Here, we report the isolation and characterization of a single-chain variable domain (scFv) antibody isolated against oligomeric Abeta using a protocol developed in our laboratory that combines phage display technology and atomic force microscopy (AFM). Starting with a randomized, single framework phage display library, after three rounds of selection against oligomeric Abeta, we identified an scFv that bound oligomeric Abeta specifically, but not monomeric or fibrillar forms. The anti-oligomeric scFv inhibits Abeta aggregation and toxicity, and reduces the toxicity of preformed oligomeric Abeta towards human neuroblastoma cells. When used to probe samples of human brain tissue, the scFv reacted with AD tissue but not a healthy control or Parkinson's disease brain samples. The anti-oligomeric Abeta scFv therefore has potential therapeutic and diagnostic applications in specifically targeting or identifying the toxic morphologies of Abeta in AD brains.

  7. Rattlesnakes are extremely fast and variable when striking at kangaroo rats in nature: Three-dimensional high-speed kinematics at night

    OpenAIRE

    Higham, Timothy E; Rulon W. Clark; Collins, Clint E.; Whitford, Malachi D.; Grace A. Freymiller

    2017-01-01

    Predation plays a central role in the lives of most organisms. Predators must find and subdue prey to survive and reproduce, whereas prey must avoid predators to do the same. The resultant antagonistic coevolution often leads to extreme adaptations in both parties. Few examples capture the imagination like a rapid strike from a venomous snake. However, almost nothing is known about strike performance of viperid snakes under natural conditions. We obtained high-speed (500?fps) three-dimensiona...

  8. [Construction and application of a lentiviral vector of single-chain variable fragment antibody against human hepatocyte growth factor receptor].

    Science.gov (United States)

    Chen, Yonghua; Guo, Jia; Yin, Yanxin; Jiang, Ming; Zhu, Hongsheng; Zhang, Guodong; Li, Bingyu

    2014-09-01

    To construct a lentiviral expression vector carrying the single-chain variable fragment (scFv) antibody against human hepatocyte growth factor receptor (HGFR), express it in transfected HEK293 cells, and observe its biological function of specific binding to antigen. The variable regions of the heavy chain (VH) and light chain (VL) genes were amplified directly from the cDNA of hybridoma cell line 8E8 secreting mouse anti-human HGFR antibody and assembled using the splice overlap extension-PCR (SOE-PCR). The constructed HGFR-scFv gene with the signal peptide SP-VH-linker-VL was ligated into the cloning vector pCR-Blunt. After cut off from pCR-Blunt using enzyme digestion, HGFR-scFv gene was subcloned into the lentiviral transfer vector pRRL-CMV, which was identified by the restriction enzyme digestion and sequencing. The lentiviral expression vector pRRL HGFR-scFv was then tansfected together with the packaging plasmids into HEK293T cells to obtain virus particles, and green fluorescent protein (GFP) expression was detected under a fluorescent microscope. Then the virus particles were used to infect HEK293 cells. The scFv expression was detected by RT-PCR and its biological affinity as antibody was measured by ELISA. The lentiviral expression vector pRRL HGFR-scFv was constructed correctly. After HEK293T cells were transfected with the pRRL HGFR-scFv plasmid, the GFP was visible. After HEK293 cells were infected with virus particles, the scFv antibody expressed could bind to HGFR specifically. The lentiviral expression vector of HGFR-scFv was constructed successfully, which would help to study the important role of HGFR in following experiments.

  9. Optimized extraction of a single-chain variable fragment of antibody by using aqueous micellar two-phase systems.

    Science.gov (United States)

    Malpiedi, Luciana P; Nerli, Bibiana B; Taqueda, Maria E S; Abdalla, Dulcineia S P; Pessoa, Adalberto

    2015-07-01

    In this work, the purification of a single-chain variable fragment (scFv) of an antibody by using liquid-liquid extraction in aqueous micellar two-phase systems was optimized by means of central composite design. Protein partitioning assays were performed by using the selected system composition in previous works: Triton X-114 at 4% wt/wt, yeast fermentation supernatant at 60% wt/wt, McIlvaine buffer pH 7.00. The other system component concentrations, Cibacron Blue F3GA (CB), Fabsorbent™ F1P HF (HF) and NaCl, were selected as independent variables. ScFv recovery percentage (%R) and purification factor (PF) were selected as the responses. According to the optimization process both, scFv recovery percentage and purification factor were favored with the addition of HF and NaCl in a range of concentrations around the central point of the second central composite design (HF 0.0120% w/w, CB 0.0200% w/w, NaCl 0.200% w/w). These experimental conditions allowed the concentration and pre-purification of scFv in the micelle-rich bottom phase of the systems with a recovery percentage superior to 88% and a purification factor of approximately 3.5. These results improved the previously presented works and demonstrated the convenience of using aqueous micellar two-phase systems as a first step in the purification of scFv molecules. Copyright © 2015 Elsevier Inc. All rights reserved.

  10. Dynamic variability of the heading-flowering stages of single rice in China based on field observations and NDVI estimations.

    Science.gov (United States)

    Zhang, Zhao; Song, Xiao; Chen, Yi; Wang, Pin; Wei, Xing; Tao, Fulu

    2015-05-01

    Although many studies have indicated the consistent impact of warming on the natural ecosystem (e.g., an early flowering and prolonged growing period), our knowledge of the impacts on agricultural systems is still poorly understood. In this study, spatiotemporal variability of the heading-flowering stages of single rice was detected and compared at three different scales using field-based methods (FBMs) and satellite-based methods (SBMs). The heading-flowering stages from 2000 to 2009 with a spatial resolution of 1 km were extracted from the SPOT/VGT NDVI time series data using the Savizky-Golay filtering method in the areas in China dominated by single rice of Northeast China (NE), the middle-lower Yangtze River Valley (YZ), the Sichuan Basin (SC), and the Yunnan-Guizhou Plateau (YG). We found that approximately 52.6 and 76.3 % of the estimated heading-flowering stages by a SBM were within ±5 and ±10 days estimation error (a root mean square error (RMSE) of 8.76 days) when compared with those determined by a FBM. Both the FBM data and the SBM data had indicated a similar spatial pattern, with the earliest annual average heading-flowering stages in SC, followed by YG, NE, and YZ, which were inconsistent with the patterns reported in natural ecosystems. Moreover, diverse temporal trends were also detected in the four regions due to different climate conditions and agronomic factors such as cultivar shifts. Nevertheless, there were no significant differences (p > 0.05) between the FBM and the SBM in both the regional average value of the phenological stages and the trends, implying the consistency and rationality of the SBM at three scales.

  11. Multisource Single-Tree Inventory in the Prediction of Tree Quality Variables and Logging Recoveries

    Directory of Open Access Journals (Sweden)

    Mikko Vastaranta

    2014-04-01

    Full Text Available The stem diameter distribution, stem form and quality information must be measured as accurately as possible to optimize cutting. For a detailed measurement of the stands, we developed and demonstrated the use of a multisource single-tree inventory (MS-STI. The two major bottlenecks in the current airborne laser scanning (ALS-based single-tree-level inventory, tree detection and tree species recognition, are avoided in MS-STI. In addition to airborne 3D data, such as ALS, MS-STI requires an existing tree map with tree species information as the input information. In operational forest management, tree mapping would be carried out after or during the first thinning. It should be highlighted that the tree map is a challenging prerequisite, but that the recent development in mobile 2D and 3D laser scanning indicates that the solution is within reach. In our study, the tested input tree map was produced by terrestrial laser scanning (TLS and by using a Global Navigation Satellite System. Predictors for tree quality attributes were extracted from ALS data or digital stereo imagery (DSI and used in the nearest-neighbor estimation approach. Stem distribution was compiled by summing the predicted single-tree measures. The accuracy of the MS-STI was validated using harvester data (timber assortments and field measures (stem diameter, tree height. RMSEs for tree height, diameter, saw log volume and pulpwood volume varied from 4.2% to 5.3%, from 10.9% to 19.9%, from 28.7% to 43.5% and from 125.1% to 134.3%, respectively. Stand-level saw log recoveries differed from −2.2% to 1.3% from the harvester measurements, as the respective differences in pulpwood recovery were between −3.0% and 10.6%. We conclude that MS-STI improves the predictions of stem-diameter distributions and provides accurate estimates for tree quality variables if an accurate tree map is available.

  12. Modulation of plumbagin production in Plumbago zeylanica using a single-chain variable fragment antibody against plumbagin.

    Science.gov (United States)

    Sakamoto, Seiichi; Putalun, Waraporn; Pongkitwitoon, Benyakan; Juengwatanatrakul, Thaweesak; Shoyama, Yukihiro; Tanaka, Hiroyuki; Morimoto, Satoshi

    2012-01-01

    A single-chain variable fragment antibody (scFv) against plumbagin (PL) accumulated the PL production in the hairy roots of Plumbago zeylanica. Recombinant Agrobacterium rhizogenes (ATCC 15834) containing an scFv gene against PL (PL-scFv) were obtained through triparental mating and transformed into P. zeylanica to induce PL-scFv protein in the hairy roots. Up to 40 μg recombinant PL-scFv were expressed per milligram of soluble protein in transgenic P. zeylanica hairy root cultures. The mean PL content obtained from transgenic hairy roots (12.24 μg/100 mg dry weight) exhibited 2.2 times higher than those obtained from wild-type (5.48 μg/100 mg dry weight). The high correlation between the PL-scFv expression level and PL content of the recombinant plants suggested that the PL biosynthesis pathway had been modulated by the expression of PL-scFv protein in the hairy roots of P. zeylanica.

  13. Single chain variable fragment antibodies block aggregation and toxicity induced by familial ALS-linked mutant forms of SOD1.

    Science.gov (United States)

    Ghadge, Ghanashyam D; Pavlovic, John D; Koduvayur, Sujatha P; Kay, Brian K; Roos, Raymond P

    2013-08-01

    Approximately 10% of amyotrophic lateral sclerosis (ALS) cases are familial (known as FALS) with an autosomal dominant inheritance pattern, and ~25% of FALS cases are caused by mutations in Cu/Zn superoxide dismutase (SOD1). There is convincing evidence that mutant SOD1 (mtSOD1) kills motor neurons (MNs) because of a gain-of-function toxicity, most likely related to aggregation of mtSOD1. A number of recent reports have suggested that antibodies can be used to treat mtSOD1-induced FALS. To follow up on the use of antibodies as potential therapeutics, we generated single chain fragments of variable region antibodies (scFvs) against SOD1, and then expressed them as 'intrabodies' within a motor neuron cell line. In the present study, we describe isolation of human scFvs that interfere with mtSOD1 in vitro aggregation and toxicity. These scFvs may have therapeutic potential in sporadic ALS, as well as FALS, given that sporadic ALS may also involve abnormalities in the SOD1 protein or activity. Copyright © 2013 Elsevier Inc. All rights reserved.

  14. The role of visual processing speed in reading speed development.

    Science.gov (United States)

    Lobier, Muriel; Dubois, Matthieu; Valdois, Sylviane

    2013-01-01

    A steady increase in reading speed is the hallmark of normal reading acquisition. However, little is known of the influence of visual attention capacity on children's reading speed. The number of distinct visual elements that can be simultaneously processed at a glance (dubbed the visual attention span), predicts single-word reading speed in both normal reading and dyslexic children. However, the exact processes that account for the relationship between the visual attention span and reading speed remain to be specified. We used the Theory of Visual Attention to estimate visual processing speed and visual short-term memory capacity from a multiple letter report task in eight and nine year old children. The visual attention span and text reading speed were also assessed. Results showed that visual processing speed and visual short term memory capacity predicted the visual attention span. Furthermore, visual processing speed predicted reading speed, but visual short term memory capacity did not. Finally, the visual attention span mediated the effect of visual processing speed on reading speed. These results suggest that visual attention capacity could constrain reading speed in elementary school children.

  15. The role of visual processing speed in reading speed development.

    Directory of Open Access Journals (Sweden)

    Muriel Lobier

    Full Text Available A steady increase in reading speed is the hallmark of normal reading acquisition. However, little is known of the influence of visual attention capacity on children's reading speed. The number of distinct visual elements that can be simultaneously processed at a glance (dubbed the visual attention span, predicts single-word reading speed in both normal reading and dyslexic children. However, the exact processes that account for the relationship between the visual attention span and reading speed remain to be specified. We used the Theory of Visual Attention to estimate visual processing speed and visual short-term memory capacity from a multiple letter report task in eight and nine year old children. The visual attention span and text reading speed were also assessed. Results showed that visual processing speed and visual short term memory capacity predicted the visual attention span. Furthermore, visual processing speed predicted reading speed, but visual short term memory capacity did not. Finally, the visual attention span mediated the effect of visual processing speed on reading speed. These results suggest that visual attention capacity could constrain reading speed in elementary school children.

  16. A 9-Week Jaques-Dalcroze Eurhythmics Intervention Improves Single and Dual-Task Gait Speed in Community-Dwelling Older People.

    Science.gov (United States)

    Ferguson-Stegall, Lisa; Vang, Mandy; Wolfe, Anthony S; Thomsen, Kathy M

    2017-09-01

    Falls are a major public health concern among older adults, and most occur while walking, especially under dualtask conditions. Jaques-Dalcroze eurhythmics (JDE) is a music-based movement training program that emphasizes multitask coordinated movement. A previous 6-mo JDE study in older people demonstrated improved gait and balance; however, the effects of short-term JDE interventions on fall risk-related outcomes are largely unknown. We conducted a preliminary investigation on whether a 9-week JDE intervention improved gait and stability in a community-dwelling older cohort, hypothesizing that improvements would occur in all outcome measures. Nine participants (78.9 ± 12.3 y) completed the supervised JDE intervention (once/week for 60 min). Gait speed was determined by the 6-m timed walk test (6MTW); dual-task gait speed was determined by another 6MTW while counting backward from 50 aloud; and coordinated stability was assessed using a Swaymeter-like device. Gait speed (0.92 ± 0.11 vs 1.04 ± 0.12 m/sec, P = .04) and dual-task gait speed (0.77 ± 0.09 vs 0.92 ± 0.11 m/sec, P = .0005) significantly improved. This novel intervention is an effective short-term physical activity option for those that plan physical activity or fall-risk reduction programs for the older people.

  17. Speed mathematics

    CERN Document Server

    Handley, Bill

    2012-01-01

    This new, revised edition of the bestselling Speed Mathematics features new chapters on memorising numbers and general information, calculating statistics and compound interest, square roots, logarithms and easy trig calculations. Written so anyone can understand, this book teaches simple strategies that will enable readers to make lightning-quick calculations. People who excel at mathematics use better strategies than the rest of us; they are not necessarily more intelligent. With Speed Mathematics you'll discover methods to make maths easy and fun. This book is perfect for stud

  18. Heat Generation During Bony Decompression of Lumbar Spinal Stenosis Using a High-Speed Diamond Drill with or without Automated Irrigation and an Ultrasonic Bone-Cutting Knife: A Single-Blinded Prospective Randomized Controlled Study.

    Science.gov (United States)

    Matthes, Marc; Pillich, Dirk Thomas; El Refaee, Ehab; Schroeder, Henry W S; Müller, Jan-Uwe

    2017-12-08

    The main objective of the present prospective, randomized, single-blinded controlled study was to measure heat during bony decompression of lumbar spinal stenosis with high-speed drills and an ultrasonic bone-cutting knife. Ninety patients diagnosed with lumbar spinal stenosis were included in this study and randomized for lumbar spinal canal decompression using either a high-speed drill with automatic irrigation, high-speed drill with manual irrigation, or an ultrasonic bone-cutting knife with automatic irrigation (USBCD). For evaluation of group homogeneity, a visual analog scale pain score and neurologic findings were measured preoperatively and postoperatively. Temperatures during bony decompression were measured using a forward-looking infrared camera system. Clinical results among the 3 groups did not differ in pain reduction, improvement of neurologic findings, or the rate of complications. However, significantly lower values were found for absolute and mean maximal temperatures during bony decompression in the USBCD group compared with the groups of patients who received the high-speed drill with automatic irrigation and the high-speed drill with manual irrigation, indicating this technique to be less aggressive in terms of thermal induction of bone necrosis. USBCD allows more precise bone removal compared with high-speed drills, and despite increased device time, no significant difference in the overall decompression time was observed. All methods examined produced short temperature peaks with possible, at least temporary, damage to bone and neural tissue. Automatic irrigation was associated with lower heat development compared with manual irrigation. Despite evidence of critical temperatures, no clinical correlation (e.g., neurologic deficits) was encountered in this study. Clinically, all 3 methods presented equally good results. Copyright © 2017 Elsevier Inc. All rights reserved.

  19. Construction and molecular characterization of mouse single-chain variable fragment antibodies against Burkholderia mallei and Burkholderia pseudomallei.

    Science.gov (United States)

    Kim, Ho San; Tsai, Shien; Zou, Nianxiang; Lo, Shyh-Ching; Wear, Douglas J; Izadjoo, Mina J

    2011-02-28

    We have selected two lipopolysaccharide (LPS) specific Burkholderia mallei mouse monoclonal antibodies (mAbs) and four anti-capsular B. pseudomallei-specific mAbs to generate mouse single-chain variable fragment (scFv) antibodies. This selection was made through extensive in vitro and in vivo assay from our library of mAbs against B. mallei and B. pseudomallei. We initially generated the mouse immunoglobulin variable heavy chain (VH) and light chain (VL) regions from each of these six selected mAbs using a phage display scFv technology. We determined the coding sequences of the VH and VL regions and successfully constructed two B. mallei-specific scFv phage antibodies consisting of two different VH (VH1 and VH2) and one Vλ1 families. Four scFvs constructed against B. pseudomallei had two VH (VH1 and VH6) and two VL (Vκ4/5 and Vκ21) families. All of six scFv antibodies constructed demonstrated good binding activity without any rounds of biopanning against B. mallei (M5D and M18F were 0.425 and 0.480 at OD405nm) and B. pseudomallei (P1E7, P2I67, P7C6, and P7F4 were 0.523, 0.859, 0.775, and 0.449 at OD405nm) by ELISA, respectively. A comparison of the immunoglobulin gene segments revealed that the gene sequences in complementarity-determining regions (CDRs) of three out of four B. pseudomallei-specific scFvs are highly conserved. We determined that the two B. mallei-specific scFvs have different CDRs in the VH, but the amino acid sequences of CDRs in the VL are conserved. This high sequence homology found in CDRs of VH or VL of these mAbs contributes to our better understanding and determination of binding to the specific antigenic epitope(s). The scFv phage display technology may be a valuable tool to develop and engineer mAbs with improved antigen-binding affinity. Copyright © 2010 Elsevier B.V. All rights reserved.

  20. Cloning approach and functional analysis of anti-intimin single-chain variable fragment (scFv

    Directory of Open Access Journals (Sweden)

    Elias Waldir P

    2011-02-01

    Full Text Available Abstract Background Intimin is an important virulence factor involved in the pathogenesis of enteropathogenic Escherichia coli (EPEC and enterohemorrhagic Escherichia coli (EHEC. Both pathogens are still important causes of diarrhea in children and adults in many developing and industrialized countries. Considering the fact that antibodies are important tools in the detection of various pathogens, an anti-intimin IgG2b monoclonal antibody was previously raised in immunized mice with the conserved sequence of the intimin molecule (int388-667. In immunoblotting assays, this monoclonal antibody showed excellent specificity. Despite good performance, the monoclonal antibody failed to detect some EPEC and EHEC isolates harboring variant amino acids within the 338-667 regions of intimin molecules. Consequently, motivated by its use for diagnosis purposes, in this study we aimed to the cloning and expression of the single-chain variable fragment from this monoclonal antibody (scFv. Findings Anti-intimin hybridoma mRNA was extracted and reversely transcripted to cDNA, and the light and heavy chains of the variable fragment of the antibody were amplified using commercial primers. The amplified chains were cloned into pGEM-T Easy vector. Specific primers were designed and used in an amplification and chain linkage strategy, obtaining the scFv, which in turn was cloned into pAE vector. E. coli BL21(DE3pLys strain was transformed with pAE scFv-intimin plasmid and subjected to induction of protein expression. Anti-intimin scFv, expressed as inclusion bodies (insoluble fraction, was denatured, purified and submitted to refolding. The protein yield was 1 mg protein per 100 mL of bacterial culture. To test the functionality of the scFv, ELISA and immunofluorescence assays were performed, showing that 275 ng of scFv reacted with 2 mg of purified intimin, resulting in an absorbance of 0.75 at 492 nm. The immunofluorescence assay showed a strong reactivity with

  1. Functional Characterization of AAV-Expressed Recombinant Anti-VEGF Single-Chain Variable Fragments In Vitro.

    Science.gov (United States)

    Wimmer, Tobias; Lorenz, Birgit; Stieger, Knut

    2015-06-01

    Most retinal neovascular disorders are caused by upregulation of vascular endothelial growth factor (VEGF) expression. These disorders are treated with repeated injections of anti-VEGF molecules, which may have severe side effects. The expression of anti-VEGF molecules by the retina itself in a controlled manner following adeno-associated viral (AAV) gene transfer could be a replacement of this therapy. The open reading frames (orf) of the light and the heavy chain of ranibizumab were cloned into an expression plasmid separated by an internal ribosomal entry site (IRES). The construct was mutated to generate ranibizumab single-chain variable fragments (scFv). Expression was verified by western blotting and the concentrations were measured with a custom-made ranibizumab ELISA. Biological activity, VEGF-binding properties, and the doxycycline-dependent induction of anti-VEGF expression were tested. An AAV2/5 vector was generated containing the optimal variant Ra02. Ra01-Ra05 molecules were detected in the cell culture medium. While the VEGF-binding affinity was significantly lower for Ra01 and Ra02 compared to Lucentis(®), the inhibition of cell migration was comparable and the maximum inhibition of Ra01 and Ra02 was reached at lower doses. The expression of Ra01 and Ra02 was shown to be regulable with the TetOn-system(®) as plasmid (Ra01, Ra02) and AAV vector construct (Ra02). Ra01 and Ra02 can be produced in eukaryotic cells after AAV-mediated gene transfer in a regulable manner in vitro and display comparable biological activity as Lucentis. These results are the basis for in vivo studies in human VEGF-overexpressing mice, a model for human neovascular disorders.

  2. A conjugate of an anti-midkine single-chain variable fragment to doxorubicin inhibits tumor growth

    Energy Technology Data Exchange (ETDEWEB)

    Zhao, Shuli [Immunology and Reproductive Biology Laboratory, Medical School & State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing (China); Nanjing Affiliated First Hospital, Nanjing Medical University, Nanjing (China); Zhao, Guangfeng; Xie, Hao; Huang, Yahong [Immunology and Reproductive Biology Laboratory, Medical School & State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing (China); Hou, Yayi [Immunology and Reproductive Biology Laboratory, Medical School & State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing (China); Jiangsu Key Laboratory of Molecular Medicine, Nanjing University, Nanjing (China)

    2012-01-27

    Doxorubicin (DOX) was conjugated to a single-chain variable fragment (scFv) against human midkine (MK), and the conjugate (scFv-DOX) was used to target the chemotherapeutic agent to a mouse solid tumor model in which the tumor cells expressed high levels of human MK. The His-tagged recombinant scFv was expressed in bacteria, purified by metal affinity chromatography, and then conjugated to DOX using oxidative dextran (Dex) as a linker. The molecular formula of this immunoconjugate was scFv(Dex){sub 1.3}(DOX){sub 20}. In vitro apoptosis assays showed that the scFv-DOX conjugate was more cytotoxic against MK-transfected human adenocarcinoma cells (BGC823-MK) than untransfected cells (55.3 ± 2.4 vs 22.4 ± 3.8%) for three independent experiments. Nude mice bearing BGC823-MK solid tumors received scFv-DOX or equivalent doses of scFv + DOX for 2 weeks and tumor growth was more effectively inhibited by the scFv-DOX conjugate than by scFv + DOX (51.83% inhibition vs 40.81%). Histological analysis of the tumor tissues revealed that the highest levels of DOX accumulated in tumors from mice treated with scFv-DOX and this resulted in more extensive tumor cell death than in animals treated with the equivalent dose of scFv + DOX. These results show that the scFv-DOX conjugate effectively inhibited tumor growth in vivo and suggest that antigen-specific scFv may be competent drug-carriers.

  3. Evaluation of single tracks of 17-4PH steel manufactured at different power densities and scanning speeds by selective laser melting

    CSIR Research Space (South Africa)

    Moller, Hein

    2016-11-01

    Full Text Available In Selective Laser Melting, the initial units produced are single tracks that overlap to create a single layer; from the sequence of layers, a 3D object is manufactured. The properties of the parts produced by SLM depend heavily on the properties...

  4. Nonlinear Speed Control of Switched Reluctance Motor Drives Taking into Account Mutual Inductance

    OpenAIRE

    Alrifai, M.; Zribi, M.; Krishnan, R.; Rayan, M.

    2008-01-01

    A speed control algorithm is proposed for variable speed switched reluctance motor (SRM) drives taking into account the effects of mutual inductances. The control scheme adopts two-phase excitation; exciting two adjacent phases can overcome the problems associated with single-phase excitation such as large torque ripple, increased acoustic noise, and rotor shaft fatigues. The effects of mutual coupling between two adjacent phases and their contribution to the generated electromagnetic torque ...

  5. Spatial layout affects speed discrimination

    Science.gov (United States)

    Verghese, P.; Stone, L. S.

    1997-01-01

    We address a surprising result in a previous study of speed discrimination with multiple moving gratings: discrimination thresholds decreased when the number of stimuli was increased, but remained unchanged when the area of a single stimulus was increased [Verghese & Stone (1995). Vision Research, 35, 2811-2823]. In this study, we manipulated the spatial- and phase relationship between multiple grating patches to determine their effect on speed discrimination thresholds. In a fusion experiment, we merged multiple stimulus patches, in stages, into a single patch. Thresholds increased as the patches were brought closer and their phase relationship was adjusted to be consistent with a single patch. Thresholds increased further still as these patches were fused into a single patch. In a fission experiment, we divided a single large patch into multiple patches by superimposing a cross with luminance equal to that of the background. Thresholds decreased as the large patch was divided into quadrants and decreased further as the quadrants were maximally separated. However, when the cross luminance was darker than the background, it was perceived as an occluder and thresholds, on average, were unchanged from that for the single large patch. A control experiment shows that the observed trend in discrimination thresholds is not due to the differences in perceived speed of the stimuli. These results suggest that the parsing of the visual image into entities affects the combination of speed information across space, and that each discrete entity effectively provides a single independent estimate of speed.

  6. Impact of loaded sit-to-stand exercises at different speeds on the physiological cost of walking in children with spastic diplegia: A single-blind randomized clinical trial.

    Science.gov (United States)

    Kusumoto, Yasuaki; Nitta, Osamu; Takaki, Kenji

    2016-10-01

    In the present study, we aimed to determine whether similarly loaded sit-to-stand exercises at different speeds improve the physiological cost of walking in children with spastic diplegia. This design was a single-blind randomized clinical trial. Sixteen children with cerebral palsy (CP), aged 12-18 years, with a diagnosis of spastic diplegia, were randomly allocated to a slow loaded sit-to-stand exercise group (n=8) and a self-paced loaded sit-to-stand exercise group (n=8). Loaded sit-to-stand exercise was conducted at home for 15min, 4 sets per day, 3-4days per week, for 6 weeks. The patients were evaluated immediately before the intervention and after the training. Lower limb muscle strength using a hand-held dynamometer, selective voluntary motor control using SCALE, 6-min walk distance (6MWD), and Physiological Cost Index (PCI) were measured. The 6MWD showed a significant difference before and after intervention. PCI showed a significant difference between the two groups and the two time points. 6MWD and the PCI improved after intervention in the slow sit-to-stand exercise group. Compared to loaded sit-to-stand exercise at a regular speed, slow low-loaded sit-to-stand exercise improved the 6MWD and PCI in children with CP, suggesting that this decrease in speed during exercise improves the physiological cost of walking in these children. Copyright © 2016 Elsevier Ltd. All rights reserved.

  7. Which angiographic variable best describes functional status 6 months after successful single-vessel coronary balloon angiopasty?

    NARCIS (Netherlands)

    B.J.W.M. Rensing (Benno); W.R.M. Hermans (Walter); P.J. de Feyter (Pim); P.W.J.C. Serruys (Patrick); J.W. Deckers (Jaap)

    1993-01-01

    textabstractOBJECTIVES. The aim of this study was to determine which quantitative angiographic variable best describes functional status 6 months after coronary balloon angioplasty. BACKGROUND. Several angiographic restenosis criteria have been developed. These can be divided into those that

  8. Speed Optimization in Liner Shipping Network Design

    DEFF Research Database (Denmark)

    Brouer, Berit Dangaard; Karsten, Christian Vad; Pisinger, David

    for the bunker consumption in the network as well as the transit time of cargo. Speed optimization has been considered for tramp shipping showing significant reductions in fuel consumption. However, variable speeds has not been considered for post optimization of the LSNDP, where speed optimization could result...

  9. Eye-Movement Parameters and Reading Speed.

    Science.gov (United States)

    Sovik, Nils; Arntzen, Oddvar; Samuelstuen, Marit

    2000-01-01

    Addresses the relationship between four eye movement parameters and reading speed of 20 twelve-year-old children during silent and oral reading. Predicts reading speed by the following variables: recognition span, average fixation duration, and number of regressive saccades. Indicates that in terms of reading speed, significant interrelationships…

  10. Spread spectrum communication and its circuit implementation using fractional-order chaotic system via a single driving variable

    Science.gov (United States)

    Cao, Hefei; Zhang, Ruoxun; Yan, Fengli

    2013-02-01

    A one-driving-variable adaptive controller for synchronization of a kind of fractional order chaotic system is designed. Based on the theory of spread spectrum communication and the synchronization of one-driving-variable fractional order chaotic system, we propose a new scheme for general spread spectrum communication. Numerical simulation and circuit experiment results are provided to illustrate the effectiveness of the proposed scheme.

  11. Intelligent Compact Drive System with a Synchronous Variable Reluctance Motor

    Directory of Open Access Journals (Sweden)

    Ioan Adrian Viorel

    2004-01-01

    Full Text Available The increase of energy costs underlines the need to use more efficient drive systems. One of the best solutions to reduce energy costs is the use of variable speed drives. They can easily match motor torque and speed to the load, saving energy when load requirements are reduced. In almost all the cases in a variable speed drive system the motor is fed by a variable frequency electronics power converter. In the last years a tendency of integrating the motor and the frequency converter into a single unit could be observed. A prototype of such a compact variable drive system with a synchronous variable reluctance motor will be presented in this paper.

  12. Increased intra-participant variability in children with autistic spectrum disorder: Evidence from single trial analyses of evoked EEG.

    Directory of Open Access Journals (Sweden)

    Elizabeth eMilne

    2011-03-01

    Full Text Available Intra-participant variability in clinical conditions such as autistic spectrum disorder (ASD is an important indicator of pathophysiological processing. The data reported here illustrate that trial-by-trial variability can be reliably measured from EEG, and that intra-participant EEG variability is significantly greater in those with ASD than in neuro-typical matched controls. EEG recorded at the scalp is a linear mixture of activity arising from muscle artifacts and numerous concurrent brain processes. To minimise these additional sources of variability, EEG data were subjected to two different methods of spatial filtering. (i The data were decomposed using infomax Independent Component Analysis (ICA, a method of blind source separation which un-mixes the EEG signal into components with maximally independent time-courses, and (ii a surface Laplacian transform was performed (Current Source Density interpolation in order to reduce the effects of volume conduction. Data are presented from thirteen high functioning adolescents with ASD without co-morbid ADHD, and twelve neuro-typical age- IQ- and gender-matched controls. Comparison of variability between the ASD and neuro-typical groups indicated that intra-participant variability of P1 latency and P1 amplitude was greater in the participants with ASD, and inter-trial α-band phase coherence was lower in the participants with ASD. These data support the suggestion that individuals with ASD are less able to synchronise the activity of stimulus-related cell assemblies than neuro-typical individuals, and provide empirical evidence in support of theories of increased neural noise in ASD.

  13. Single-step purification of dracorhodin from dragon's blood resin of Daemonorops draco using high-speed counter-current chromatography combined with pH modulation.

    Science.gov (United States)

    Shi, Jianmei; Hu, Ruilin; Lu, Yanbin; Sun, Cuirong; Wu, Tianxing

    2009-12-01

    Dracorhodin is a major constituent found in "Dragon's blood" resin of Daemonorops draco Willd. Blume. This natural flavylium compound is a potent pharmaceutical substance due to its biological and pharmacological activities such as antimicrobial, antiviral, antitumor and cytotoxic activity. An effective high-speed counter-current chromatography method was successfully established for the isolation and purification of dracorhodin directly from extract of D. draco by using a two-phase solvent system composed of n-hexane/ethyl acetate/methanol/water (2:3:2:3 v/v). Under the optimal conditions, 6.6 mg dracorhodin was obtained from 100 mg crude resin. The isolated fraction of counter-current chromatography was determined by HPLC, NMR, UV/visible and ESI/MS combined with pH modulation, since dracorhodin is unstable in solution which exists in different forms depending on pH values. The data were compared with those of the reference substance, and the literatures as well. The purity of dracorhodin was over 98% based on the HPLC result.

  14. Variability in disease phenotypes within a single PRNP genotype suggests the existence of multiple natural sheep scarpie strains within Europe

    NARCIS (Netherlands)

    González, L.; Sisó, S.; Monleón, E.; Casalone, C.; Keulen, van L.J.M.; Balkema-Buschmann, A.; Ortiz-Peláez, A.; Lulini, B.; Langeveld, J.P.M.; Hoffmann, C.; Badiola, J.J.; Jeffrey, M.; Acín, C.

    2010-01-01

    Variability of pathological phenotypes within classical sheep scrapie cases has been reported for some time, but in many instances it has been attributed to differences in the PRNP genotype of the host. To address this issue we have examined by immunohistochemistry (IHC) and Western blotting (WB)

  15. [Influence of gait speed on plantar pressure in subjects with unilateral knee osteoarthritis].

    Science.gov (United States)

    Fernandes, Wagner Costa; Machado, Alvaro; Borella, Caio; Carpes, Felipe P

    2014-01-01

    Changing gait speed is a common strategy to manipulate exercise intensity during physical exercise, but may elicit higher impact forces and consequent joint loading. Here we analyzed the effects of increasing walking velocity on plantar pressure and asymmetries in elderly with knee osteoarthritis (OA). Our hypothesis was that the contralateral limb could receive higher loading compared to the OA limb in the different walking speeds tested. Twelve elderly with unilateral knee OA walked at different self-selected speeds along a 10 m pass way stepping on an instrumented mat for measurement of plantar pressure at preferred, slow and fast gait speeds. Five steps were recorded for each speed. Plantar pressure data were compared between the speeds and legs. speeds were significantly different between them (p<0.05). Mean and peak plantar pressure increased when speed changed from slow to fast (p<0.05). Velocity of the center of pressure increased and the single stance time decreased when walk speed was increased (p<0.05). Any asymmetries were observed. Increasing gait speed from slow to fast in subjects with unilateral knee OA significantly affected variables of plantar pressure, but asymmetries between committed and contralateral leg were not detected. Copyright © 2014 Elsevier Editora Ltda. All rights reserved.

  16. Speed enforcement in Norway

    DEFF Research Database (Denmark)

    Elvik, Rune

    2015-01-01

    the game-theoretic model empirically. Testing the model rigorously is difficult, mainly because some of the relevant variables are not reliably measured and are endogenous. Two models were developed: one to identify sources of changes in the rate of violations, one to identify sources of changes......This paper probes the relationship between changes in the risk of apprehension for speeding in Norway and changes in the amount of speeding. The paper is based on a game-theoretic model of how the rate of violations and the amount of enforcement is determined by the interaction between drivers...... and the police. This model makes predictions both about how drivers will adapt to changes in the amount of enforcement (the more enforcement, the less violations) as well as how the police will adapt to changes in the rate of violations (the less violations, the less enforcement). The paper attempts to test...

  17. Pre-stimulus Alpha Oscillations and Inter-subject Variability of Motor Evoked Potentials in Single- and Paired-Pulse TMS Paradigms.

    Science.gov (United States)

    Iscan, Zafer; Nazarova, Maria; Fedele, Tommaso; Blagovechtchenski, Evgeny; Nikulin, Vadim V

    2016-01-01

    Inter- and intra-subject variability of the motor evoked potentials (MEPs) to TMS is a well-known phenomenon. Although a possible link between this variability and ongoing brain oscillations was demonstrated, the results of the studies are not consistent with each other. Exploring this topic further is important since the modulation of MEPs provides unique possibility to relate oscillatory cortical phenomena to the state of the motor cortex probed with TMS. Given that alpha oscillations were shown to reflect cortical excitability, we hypothesized that their power and variability might explain the modulation of subject-specific MEPs to single- and paired-pulse TMS (spTMS, ppTMS, respectively). Neuronal activity was recorded with multichannel electroencephalogram. We used spTMS and two ppTMS conditions: intracortical facilitation (ICF) and short-interval intracortical inhibition (SICI). Spearman correlations were calculated within and across subjects between MEPs and the pre-stimulus power of alpha oscillations in low (8-10 Hz) and high (10-12 Hz) frequency bands. Coefficient of quartile variation was used to measure variability. Across-subject analysis revealed no difference in the pre-stimulus alpha power among the TMS conditions. However, the variability of high-alpha power in spTMS condition was larger than in the SICI condition. In ICF condition pre-stimulus high-alpha power variability correlated positively with MEP amplitude variability. No correlation has been observed between the pre-stimulus alpha power and MEP responses in any of the conditions. Our results show that the variability of the alpha oscillations can be more predictive of TMS effects than the commonly used power of oscillations and we provide further support for the dissociation of high and low-alpha bands in predicting responses produced by the stimulation of the motor cortex.

  18. Numerical modelling of a high-speed rigid rotor in a single-aerostatic bearing using modified Euler equations of motion

    Science.gov (United States)

    Frew, D. A.; Scheffer, C.

    2008-01-01

    Accurate rotordynamic analysis is critical in the achievement of efficient rotary machine design, however the majority of models concern flexible shafts with concentrated supports. The modified Euler equations of motion are used in a numerical model to calculate the natural frequencies and whirl amplitudes of a rigid rotor supported by a single-aerostatic bearing. The bearing is modelled with a non-constant stiffness distribution along its length and a non-symmetric centre of gravity. The results are compared with experimental modal analysis (EMA).

  19. A Comparative Analysis of Speed Profile Models for Ankle Pointing Movements: Evidence that Lower and Upper Extremity Discrete Movements are controlled by a Single Invariant Strategy

    Directory of Open Access Journals (Sweden)

    Konstantinos eMichmizos

    2014-11-01

    Full Text Available Little is known about whether our knowledge of how the central nervous system controls the upper extremities, can generalize, and to what extent to the lower limbs. Our continuous efforts to design the ideal adaptive robotic therapy for the lower limbs of stroke patients and children with cerebral palsy highlighted the importance of analyzing and modeling the kinematics of the lower limbs, in general, and those of the ankle joints, in particular. We recruited 15 young healthy adults that performed in total 1,386 visually-evoked, visually-guided and target-directed discrete pointing movements with their ankle in dorsal–plantar and inversion–eversion directions. Using a nonlinear, least-squares error-minimization procedure, we estimated the parameters for 19 models which were initially designed to capture the dynamics of upper limb movements of various complexity. We validated our models based on their ability to reconstruct the experimental data. Our results suggest a remarkable similarity between the top performing models that described the speed profiles of ankle pointing movements and the ones previously found for the upper extremities both during arm reaching and wrist pointing movements. Among the top performers were the support-bounded lognormal and the beta models that have a neurophysiological basis and have been successfully used in upper extremity studies with normal subjects and patients. Our findings suggest that the same model can be applied to different human hardware, perhaps revealing a key invariant in human motor control. These findings have a great potential to enhance our rehabilitation efforts in any population with lower extremity deficits by, for example, assessing the level of motor impairment and improvement as well as informing the design of control algorithms for therapeutic ankle robots.

  20. Simultaneous 10 Gbps data and polarization-based pulse-per-second clock transmission using a single VCSEL for high-speed optical fibre access networks

    Science.gov (United States)

    Isoe, G. M.; Wassin, S.; Gamatham, R. R. G.; Leitch, A. W. R.; Gibbon, T. B.

    2017-01-01

    Access networks based on vertical cavity surface emitting laser (VCSEL) transmitters offer alternative solution in delivering different high bandwidth, cost effective services to the customer premises. Clock and reference frequency distribution is critical for applications such as Coordinated Universal Time (UTC), GPS, banking and big data science projects. Simultaneous distribution of both data and timing signals over shared infrastructure is thus desirable. In this paper, we propose and experimentally demonstrate a novel, cost-effective technique for multi-signal modulation on a single VCSEL transmitter. Two signal types, an intensity modulated 10 Gbps data signal and a polarization-based pulse per second (PPS) clock signal are directly modulated onto a single VCSEL carrier at 1310 nm. Spectral efficiency is maximized by exploiting inherent orthogonal polarization switching of the VCSEL with changing bias in transmission of the PPS signal. A 10 Gbps VCSEL transmission with PPS over 11 km of G.652 fibre introduced a transmission penalty of 0.52 dB. The contribution of PPS to this penalty was found to be 0.08 dB.

  1. Rapid assessment of different oxygenic phototrophs and single-cell photosynthesis with multicolour variable chlorophyll fluorescence imaging

    DEFF Research Database (Denmark)

    Trampe, Erik Christian Løvbjerg; Kolbowski, J.; Schreiber, U.

    2011-01-01

    , red or white light. Automated sequential exposure of microscopic samples to the three excitation colours enables subsequent deconvolution of the resulting fluorescence signals and colour marking of cells with different photopigmentation, i.e., cyanobacteria, green algae, red algae and diatoms......We present a new system for microscopic multicolour variable chlorophyll fluorescence imaging of aquatic phototrophs. The system is compact and portable and enables microscopic imaging of photosynthetic performance of individual cells and chloroplasts using different combinations of blue, green...

  2. Variability of tsunami inundation footprints considering stochastic scenarios based on a single rupture model: Application to the 2011 Tohoku earthquake

    KAUST Repository

    Goda, Katsuichiro

    2015-06-30

    The sensitivity and variability of spatial tsunami inundation footprints in coastal cities and towns due to a megathrust subduction earthquake in the Tohoku region of Japan are investigated by considering different fault geometry and slip distributions. Stochastic tsunami scenarios are generated based on the spectral analysis and synthesis method with regards to an inverted source model. To assess spatial inundation processes accurately, tsunami modeling is conducted using bathymetry and elevation data with 50 m grid resolutions. Using the developed methodology for assessing variability of tsunami hazard estimates, stochastic inundation depth maps can be generated for local coastal communities. These maps are important for improving disaster preparedness by understanding the consequences of different situations/conditions, and by communicating uncertainty associated with hazard predictions. The analysis indicates that the sensitivity of inundation areas to the geometrical parameters (i.e., top-edge depth, strike, and dip) depends on the tsunami source characteristics and the site location, and is therefore complex and highly nonlinear. The variability assessment of inundation footprints indicates significant influence of slip distributions. In particular, topographical features of the region, such as ria coast and near-shore plain, have major influence on the tsunami inundation footprints.

  3. VARIABLE SPEED INTEGRATED INTELLIGENT HVAC BLOWER

    Energy Technology Data Exchange (ETDEWEB)

    Shixiao Wang; Herman Wiegman; Wilson Wu; John Down; Luana Iorio; Asha Devarajan; Jing Wang; Ralph Carl; Charlie Stephens; Jeannine Jones; Paul Szczesny

    2001-11-14

    This comprehensive topical report discusses the key findings in the development of a intelligent integrated blower for HVAC applications. The benefits of rearward inclined blades over that of traditional forward inclined blades is well documented and a prototype blower design is presented. A comparison of the proposed blower to that of three typical units from the industry is presented. The design of the blower housing is also addressed and the impact of size limitations on static efficiency is discussed. Issues of air flow controllability in the rearward inclined blower is addressed and a solution to this problem is proposed. Several motor design options are discussed including inside-out radial flux designs and novel axial flux designs, all are focused on the various blower needs. The control of the motor-blower and airflow through the use of a high density inverter stage and modern digital signal processor is presented. The key technical challenges of the approach are discussed. The use of the motor as a sensor in the larger heating/ventilating system is also discussed. Diagnostic results for both the motor itself and the blower system are presented.

  4. Exploiting the intra-subject latency variability from single-trial event-related potentials in the P3 time range: A review and comparative evaluation of methods.

    Science.gov (United States)

    Ouyang, Guang; Hildebrandt, Andrea; Sommer, Werner; Zhou, Changsong

    2017-04-01

    The intra-subject variability (ISV) in brain responses during cognitive processing across experimental trials has been recognised as an important facet of neural functionality reflecting an intrinsic neurophysiological characteristic of the brain. In recent decades, ISV in behaviour has been found to be significantly associated with cognitive functioning varying across individuals, development, ages, and pathological conditions. Event-related potentials (ERPs) measured in single trials are important tools for characterizing ISV at the neural level. However, due to the overlapping spectra of noise and signals, the retrieval of information from single-trial ERPs related to cognitive processing has been a challenge. We review the major problems that researchers face in the estimation of ISV in single-trial ERPs. Then, we present an extensive evaluation of several methods of single-trial latency estimation based on both simulated and real data. The relationships of ISV in ERPs and reaction times are compared between the different single-trial methods to assess their relative efficiency in predicting task performance from neural signals. The pros and cons of the methods are discussed. Copyright © 2017 Elsevier Ltd. All rights reserved.

  5. Efficacy and speed of onset of pain relief of fast-dissolving paracetamol on postsurgical dental pain: two randomized, single-dose, double-blind, placebo-controlled clinical studies.

    Science.gov (United States)

    Yue, Yong; Collaku, Agron; Brown, Jean; Buchanan, William L; Reed, Kenneth; Cooper, Stephen A; Otto, James

    2013-09-01

    Paracetamol (APAP), also known as acetaminophen, is the most commonly used over-the-counter analgesic for the treatment of mild-to-moderate pain. However, the speed of onset of pain relief is limited mainly to the standard, immediate-release formulation. Efficacy and speed of onset of pain relief are critical in acute pain situations such as postsurgical dental pain, because reducing pain can improve clinical outcome and reduce the risk of transition from acute pain to more chronic pain. Efficacy and rapid onset also reduce the risk of excessive dosing with the analgesic. We sought to investigate the dose-response efficacy and speed of onset of pain relief of a fast-dissolving APAP formulation compared with lower doses of APAP and placebo in dental patients after impacted third molar extraction. Two single-center, single-dose, randomized, placebo-controlled, double-blind, double-dummy, parallel-group studies (Study I and Study II) were conducted to evaluate the efficacy and speed of onset of pain relief of different doses of a fast-dissolving APAP tablet (FD-APAP), standard APAP, and placebo in patients with postsurgical dental pain following third molar extraction. In Study I, a single dose of FD-APAP 1000 mg, FD-APAP 500 mg, or placebo was given to 300 patients; in Study II, a single dose of FD-APAP 1000 mg, standard APAP 650 mg, or placebo was given to 401 patients. All 701 patients from both studies were included in the analysis and safety assessment. FD-APAP 1000 mg demonstrated significantly greater effect compared with FD-APAP 500 mg, APAP 650 mg, and placebo for all efficacy measurements, including sum of pain relief and pain intensity difference, total pain relief, sum of pain intensity difference, pain intensity difference, and pain relief score during 6 hours after the dose. Onset of confirmed first perceptible relief in subjects treated with FD-APAP 1000 mg was 15 minutes, which was 32% and 25% significantly shorter than onset of pain relief of FD

  6. Statistical frequency-dependent analysis of trial-to-trial variability in single time series by recurrence plots

    Directory of Open Access Journals (Sweden)

    Tamara eTosic

    2016-01-01

    Full Text Available For decades, research in neuroscience has supported the hypothesis that brain dynamics exhibits recurrent metastable states connected by transients, which together encode fundamental neural information processing. To understand the system’s dynamics it is important to detect such recurrence domains, but it is challenging to extract them from experimental neuroscience datasets due to the large trial-to-trial variability. The proposed methodology extracts recurrent metastable states in univariate time series by transforming datasets into their time-frequency representations and computing recurrence plots based on instantaneous spectral power values in various frequency bands. Additionally, a new statistical inference analysis compares different trial recurrence plots with corresponding surrogates to obtain statistically significant recurrent structures. This combination of methods is validated by applying it to two artificial datasets. In a final study of visually-evoked Local Field Potentials in partially anesthetized ferrets, the methodology is able to reveal recurrence structures of neural responses with trial-to-trial variability. Focusing on different frequency bands, the delta-band activity is much less recurrent than alpha-band activity. Moreover, alpha-activity is susceptible to pre-stimuli, while delta-activity is much less sensitive to pre-stimuli. This difference in recurrence structures in different frequency bands indicates diverse underlying information processing steps in the brain.

  7. Design Optimization and the Limits of Steady-State Heating Efficiency for Conventional Single-Speed Air-Source Heat Pumps

    Energy Technology Data Exchange (ETDEWEB)

    Rice, C.K.

    2001-06-06

    The ORNL Heat Pump Model and an optimizing program were used to explore the limits of steady-state heating efficiency for conventional air-source heat pumps. The method used allows for the simultaneous optimization of ten selected design variables, taking proper account of their interactions, while constraining other parameters to chosen limits or fixed values. Designs were optimized for a fixed heating capacity, but the results may be scaled to other capacities. Substantial performance improvement is predicted compared to today's state of the art heat pump. With increased component efficiencies that are expected in the near future and with modest increases in heat exchanger area, a 28% increase in heating efficiency is predicted; for long-term improvements with considerably larger heat exchangers, a 56% increase is possible. The improved efficiencies are accompanied by substantial reductions in the requirements for compressor and motor size. The predicted performance improvements are attributed not only to improved components and larger heat exchangers but also to the use of an optimizing design procedure. Deviations from the optimized design may be necessary to make use of available component sizes and to maintain good cooling-mode performance while improving the heating efficiency. Sensitivity plots (i.e., COP as a function of one or more design parameters) were developed to explore design flexibilities and to evaluate their consequences. The performance of the optimized designs was compared to that of modified ideal cycles to assess the factors that limit further improvement. It is hoped that the design methods developed will be useful to designers in the heat pump industry.

  8. Combined Flux Observer With Signal Injection Enhancement for Wide Speed Range Sensorless Direct Torque Control of IPMSM Drives

    DEFF Research Database (Denmark)

    Blaabjerg, Frede; Andreescu, G.-D.; Pitic, C.I.

    2008-01-01

    This paper proposes a motion-sensorless control system using direct torque control with space vector modulation for interior permanent magnet synchronous motor (IPMSM) drives, for wide speed range operation, including standstill. A novel stator flux observer with variable structure uses a combined...... correction depending on estimated torque, is proposed and tested. Extensive simulation results and significant experimental results provided good performance for the proposed IPMSM sensorless system in more than 1:1000 speed range, under full-load operation, from very low speeds (1 r/min experimental) up...... voltage-current model with PI compensator for low-speed operations. As speed increases, the observer switches gradually to a PI compensated closed-loop voltage model, which is solely used at high speeds. High-frequency rotating-voltage injection with a single D-module bandpass vector filter and a phase...

  9. Using machine learning to speed up manual image annotation: application to a 3D imaging protocol for measuring single cell gene expression in the developing C. elegans embryo

    Directory of Open Access Journals (Sweden)

    Waterston Robert H

    2010-02-01

    Full Text Available Abstract Background Image analysis is an essential component in many biological experiments that study gene expression, cell cycle progression, and protein localization. A protocol for tracking the expression of individual C. elegans genes was developed that collects image samples of a developing embryo by 3-D time lapse microscopy. In this protocol, a program called StarryNite performs the automatic recognition of fluorescently labeled cells and traces their lineage. However, due to the amount of noise present in the data and due to the challenges introduced by increasing number of cells in later stages of development, this program is not error free. In the current version, the error correction (i.e., editing is performed manually using a graphical interface tool named AceTree, which is specifically developed for this task. For a single experiment, this manual annotation task takes several hours. Results In this paper, we reduce the time required to correct errors made by StarryNite. We target one of the most frequent error types (movements annotated as divisions and train a support vector machine (SVM classifier to decide whether a division call made by StarryNite is correct or not. We show, via cross-validation experiments on several benchmark data sets, that the SVM successfully identifies this type of error significantly. A new version of StarryNite that includes the trained SVM classifier is available at http://starrynite.sourceforge.net. Conclusions We demonstrate the utility of a machine learning approach to error annotation for StarryNite. In the process, we also provide some general methodologies for developing and validating a classifier with respect to a given pattern recognition task.

  10. Variability of virgin olive oil phenolic compounds in a segregating progeny from a single cross in Olea europaea L. and sensory and nutritional quality implications.

    Science.gov (United States)

    Pérez, Ana G; León, Lorenzo; Pascual, Mar; Romero-Segura, Carmen; Sánchez-Ortiz, Araceli; de la Rosa, Raúl; Sanz, Carlos

    2014-01-01

    Virgin olive oil phenolic compounds are responsible for its nutritional and sensory quality. The synthesis of phenolic compounds occurs when enzymes and substrates meet as olive fruit is crushed during the industrial process to obtain the oil. The genetic variability of the major phenolic compounds of virgin olive oil was studied in a progeny of the cross of Picual x Arbequina olive cultivars (Olea europaea L.). They belong to four different groups: compounds that included tyrosol or hydroxytyrosol in their molecules, lignans, flavonoids, and phenolic acids. Data of phenolics in the oils showed that the progeny displayed a large degree of variability, widely transgressing the genitor levels. This high variability can be of interest on breeding programs. Thus, multivariate analysis allowed to identify genotypes within the progeny particularly interesting in terms of phenolic composition and deduced organoleptic and nutritional quality. The present study has demonstrated that it is possible to obtain enough degree of variability with a single cross of olive cultivars for compounds related to the nutritional and organoleptic properties of virgin olive oil.

  11. Variability of Virgin Olive Oil Phenolic Compounds in a Segregating Progeny from a Single Cross in Olea europaea L. and Sensory and Nutritional Quality Implications

    Science.gov (United States)

    Pérez, Ana G.; León, Lorenzo; Pascual, Mar; Romero-Segura, Carmen; Sánchez-Ortiz, Araceli; de la Rosa, Raúl; Sanz, Carlos

    2014-01-01

    Virgin olive oil phenolic compounds are responsible for its nutritional and sensory quality. The synthesis of phenolic compounds occurs when enzymes and substrates meet as olive fruit is crushed during the industrial process to obtain the oil. The genetic variability of the major phenolic compounds of virgin olive oil was studied in a progeny of the cross of Picual x Arbequina olive cultivars (Olea europaea L.). They belong to four different groups: compounds that included tyrosol or hydroxytyrosol in their molecules, lignans, flavonoids, and phenolic acids. Data of phenolics in the oils showed that the progeny displayed a large degree of variability, widely transgressing the genitor levels. This high variability can be of interest on breeding programs. Thus, multivariate analysis allowed to identify genotypes within the progeny particularly interesting in terms of phenolic composition and deduced organoleptic and nutritional quality. The present study has demonstrated that it is possible to obtain enough degree of variability with a single cross of olive cultivars for compounds related to the nutritional and organoleptic properties of virgin olive oil. PMID:24651694

  12. Development of single chain variable fragment (scFv) antibodies against Xylella fastidiosa subsp. pauca by phage display.

    Science.gov (United States)

    Yuan, Qing; Jordan, Ramon; Brlansky, Ronald H; Istomina, Olga; Hartung, John

    2015-10-01

    Xylella fastidiosa is a member of the gamma proteobacteria. It is fastidious, insect-vectored and xylem-limited and causes a variety of diseases, some severe, on a wide range of economically important perennial crops, including grape and citrus. Antibody based detection assays are commercially available for X. fastidiosa, and are effective at the species, but not at the subspecies level. We have made a library of scFv antibody fragments directed against X. fastidiosa subsp. pauca strain 9a5c (citrus) by using phage display technology. Antibody gene repertoires were PCR-amplified using 23 primers for the heavy chain variable region (V(H)) and 21 primers for the light chain variable region (V(L)). The V(H) and V(L) were joined by overlap extension PCR, and then the genes of the scFv library were ligated into the phage vector pKM19. The library contained 1.2×10(7) independent clones with full-length scFv inserts. In each of 3cycles of affinity-selection with 9a5c, about 1.0×10(12) phage were used for panning with 4.1×10(6), 7.1×10(6), 2.1×10(7) phage recovered after the first, second and third cycles, respectively. Sixty-six percent of clones from the final library bound X. fastidiosa 9a5c in an ELISA. Some of these scFv antibodies recognized strain 9a5c and did not recognize X. fastidiosa strains that cause Pierce's disease of grapevine. Published by Elsevier B.V.

  13. Variable temperature and high-pressure crystal chemistry of perovskite formamidinium lead iodide: a single crystal X-ray diffraction and computational study.

    Science.gov (United States)

    Sun, Shijing; Deng, Zeyu; Wu, Yue; Wei, Fengxia; Halis Isikgor, Furkan; Brivio, Federico; Gaultois, Michael W; Ouyang, Jianyong; Bristowe, Paul D; Cheetham, Anthony K; Kieslich, Gregor

    2017-07-04

    We investigate the variable temperature (100-450 K) and high-pressure (p = ambient - 0.74 GPa) crystal chemistry of the black perovskite formamidinium lead iodide, [(NH2)2CH]PbI3, using single crystal X-ray diffraction. In both cases we find a phase transition to a tetragonal phase. Our experimental results are combined with first principles calculations, providing information about the electronic properties of [(NH2)2CH]PbI3 as well as the most probable orientation of the [(NH2)2CH](+) cations.

  14. Ptychographic ultrahigh-speed imaging.

    Science.gov (United States)

    Sidorenko, Pavel; Lahav, Oren; Cohen, Oren

    2017-05-15

    We propose and demonstrate numerically a simple method for ultrahigh-speed imaging of complex (amplitude and phase) samples. Our method exploits redundancy in single-shot ptychography (SSP) for reconstruction of multiple frames from a single camera snapshot. We term the method Time-resolved Imaging by Multiplexed Ptychography (TIMP). We demonstrate TIMP numerically-reconstructing 15 frames of a complexed-valued dynamic object from a single noisy camera snapshot. Experimentally, we demonstrate SSP with single pulse illumination with pulse duration of 150 psec, where its spectral bandwidth can support 30 fsec pulses.

  15. High-Speed Electrochemical Imaging.

    Science.gov (United States)

    Momotenko, Dmitry; Byers, Joshua C; McKelvey, Kim; Kang, Minkyung; Unwin, Patrick R

    2015-09-22

    The design, development, and application of high-speed scanning electrochemical probe microscopy is reported. The approach allows the acquisition of a series of high-resolution images (typically 1000 pixels μm(-2)) at rates approaching 4 seconds per frame, while collecting up to 8000 image pixels per second, about 1000 times faster than typical imaging speeds used up to now. The focus is on scanning electrochemical cell microscopy (SECCM), but the principles and practicalities are applicable to many electrochemical imaging methods. The versatility of the high-speed scan concept is demonstrated at a variety of substrates, including imaging the electroactivity of a patterned self-assembled monolayer on gold, visualization of chemical reactions occurring at single wall carbon nanotubes, and probing nanoscale electrocatalysts for water splitting. These studies provide movies of spatial variations of electrochemical fluxes as a function of potential and a platform for the further development of high speed scanning with other electrochemical imaging techniques.

  16. Generation and characterization of a human single-chain fragment variable (scFv antibody against cytosine deaminase from Yeast

    Directory of Open Access Journals (Sweden)

    Tombesi Marina

    2008-09-01

    Full Text Available Abstract Background The ability of cytosine deaminase (CD to convert the antifungal agent 5-fluorocytosine (5-FC into one of the most potent and largely used anticancer compound such as 5-fluorouracil (5-FU raised considerable interest in this enzyme to model gene or antibody – directed enzyme-prodrug therapy (GDEPT/ADEPT aiming to improve the therapeutic ratio (benefit versus toxic side-effects of cancer chemotherapy. The selection and characterization of a human monoclonal antibody in single chain fragment (scFv format represents a powerful reagent to allow in in vitro and in vivo detection of CD expression in GDEPT/ADEPT studies. Results An enzymatic active recombinant CD from yeast (yCD was expressed in E. coli system and used as antigen for biopanning approach of the large semi-synthetic ETH-2 antibody phage library. Several scFvs were isolated and specificity towards yCD was confirmed by Western blot and ELISA. Further, biochemical and functional investigations demonstrated that the binding of specific scFv with yCD did not interfere with the activity of the enzyme in converting 5-FC into 5-FU. Conclusion The construction of libraries of recombinant antibody fragments that are displayed on the surface of filamentous phage, and the selection of phage antibodies against target antigens, have become an important biotechnological tool in generating new monoclonal antibodies for research and clinical applications. The scFvH5 generated by this method is the first human antibody which is able to detect yCD in routinary laboratory techniques without interfering with its enzymatic function.

  17. Continental pollution in the Western Mediterranean basin: large variability of the aerosol single scattering albedo and influence on the direct shortwave radiative effect

    Directory of Open Access Journals (Sweden)

    C. Di Biagio

    2016-08-01

    Full Text Available Pollution aerosols strongly influence the composition of the Western Mediterranean basin, but at present little is known on their optical properties. We report in this study in situ observations of the single scattering albedo (ω of pollution aerosol plumes measured over the Western Mediterranean basin during the TRAQA (TRansport and Air QuAlity airborne campaign in summer 2012. Cases of pollution export from different source regions around the basin and at different altitudes between  ∼  160 and 3500 m above sea level were sampled during the flights. Data from this study show a large variability of ω, with values between 0.84–0.98 at 370 nm and 0.70–0.99 at 950 nm. The single scattering albedo generally decreases with the wavelength, with some exception associated to the mixing of pollution with sea spray or dust particles over the sea surface. The lowest values of ω (0.84–0.70 between 370 and 950 nm are measured in correspondence of a fresh plume possibly linked to ship emissions over the basin. The range of variability of ω observed in this study seems to be independent of the source region around the basin, as well as of the altitude and aging time of the plumes. The observed variability of ω reflects in a large variability for the complex refractive index of pollution aerosols, which is estimated to span in the large range 1.41–1.77 and 0.002–0.097 for the real and the imaginary parts, respectively, between 370 and 950 nm. Radiative calculations in clear-sky conditions were performed with the GAME radiative transfer model to test the sensitivity of the aerosol shortwave Direct Radiative Effect (DRE to the variability of ω as observed in this study. Results from the calculations suggest up to a 50 and 30 % change of the forcing efficiency (FE, i.e. the DRE per unit of optical depth, at the surface (−160/−235 W m−2 τ−1 at 60° solar zenith angle and at the Top-Of-Atmosphere (−137/−92

  18. "Dial-an-ROI?" changing basic variables impacts cost trends in single-population pre-post ("DMAA type") savings analysis.

    Science.gov (United States)

    Juster, Iver A; Rosenberg, Stephen N; Senapati, Deeptimayee; Shah, Mayur R

    2009-02-01

    Disease management (DM) programs claim to achieve cost savings by reducing clinical adverse events. While measuring changes in adverse events is straightforward, plausibly demonstrating savings has been contentious, especially absent an external comparison population. In this situation, a single-population methodology is often used, in which the cost trend for those with no program conditions ("non-chronics"--NC) forms the expected trend for those who have at least 1 program condition ("chronics"--C). The methodology's fundamental assumption is that--absent DM--C and NC trends would be identical (or bear a constant relationship over time). We assessed this assumption by altering the values of key variables used to identify C and NC, and to calculate trend. We compared C and NC baseline trends for a 23-condition telephonic DM multiemployer program representing nearly 300,000 members. Trends were calculated for 16 combinations of values for 4 key variables: identification look-back frame (12 vs. 24 months); identification threshold (high vs. lower specificity); claims runout (3 vs. 6 months); and minimum required insurance eligibility (any 6 months vs. 12 months continuous eligibility in the measurement year). Identification was performed by annual qualification. Changes in values for the 4 key variables markedly impacted baseline C and NC trends. C trends varied between 10.1% and 13.1%; NC trends between 5.2% and 12.8%. C-NC trend differences ranged between -1.9% and +7.0%. The combination of 24 months identification look-back, high identification threshold, 6 months runout, and any-6-months eligibility gave the most convergent C and NC trends (10.4% and 10.7%). Seemingly minor changes in key variables impact C and NC trends in single-population pre-post DM savings methodologies. When a suitable comparison population is not available, at least 1 year of baseline C and NC trends should be reported-as recommended by the DMAA--and values of key variables used should be

  19. Continental pollution in the Western Mediterranean basin: large variability of the aerosol single scattering albedo and influence on the direct shortwave radiative effect

    Science.gov (United States)

    Di Biagio, Claudia; Formenti, Paola; Doppler, Lionel; Gaimoz, Cécile; Grand, Noel; Ancellet, Gerard; Attié, Jean-Luc; Bucci, Silvia; Dubuisson, Philippe; Fierli, Federico; Mallet, Marc; Ravetta, François

    2016-08-01

    Pollution aerosols strongly influence the composition of the Western Mediterranean basin, but at present little is known on their optical properties. We report in this study in situ observations of the single scattering albedo (ω) of pollution aerosol plumes measured over the Western Mediterranean basin during the TRAQA (TRansport and Air QuAlity) airborne campaign in summer 2012. Cases of pollution export from different source regions around the basin and at different altitudes between ˜ 160 and 3500 m above sea level were sampled during the flights. Data from this study show a large variability of ω, with values between 0.84-0.98 at 370 nm and 0.70-0.99 at 950 nm. The single scattering albedo generally decreases with the wavelength, with some exception associated to the mixing of pollution with sea spray or dust particles over the sea surface. The lowest values of ω (0.84-0.70 between 370 and 950 nm) are measured in correspondence of a fresh plume possibly linked to ship emissions over the basin. The range of variability of ω observed in this study seems to be independent of the source region around the basin, as well as of the altitude and aging time of the plumes. The observed variability of ω reflects in a large variability for the complex refractive index of pollution aerosols, which is estimated to span in the large range 1.41-1.77 and 0.002-0.097 for the real and the imaginary parts, respectively, between 370 and 950 nm. Radiative calculations in clear-sky conditions were performed with the GAME radiative transfer model to test the sensitivity of the aerosol shortwave Direct Radiative Effect (DRE) to the variability of ω as observed in this study. Results from the calculations suggest up to a 50 and 30 % change of the forcing efficiency (FE), i.e. the DRE per unit of optical depth, at the surface (-160/-235 W m-2 τ-1 at 60° solar zenith angle) and at the Top-Of-Atmosphere (-137/-92 W m-2 τ-1) for ω varying between its maximum and minimum value

  20. Assessing the Impact of Forest Change and Climate Variability on Dry Season Runoff by an Improved Single Watershed Approach: A Comparative Study in Two Large Watersheds, China

    Directory of Open Access Journals (Sweden)

    Yiping Hou

    2018-01-01

    Full Text Available Extensive studies on hydrological responses to forest change have been published for centuries, yet partitioning the hydrological effects of forest change, climate variability and other factors in a large watershed remains a challenge. In this study, we developed a single watershed approach combining the modified double mass curve (MDMC and the time series multivariate autoregressive integrated moving average model (ARIMAX to separate the impact of forest change, climate variability and other factors on dry season runoff variation in two large watersheds in China. The Zagunao watershed was examined for the deforestation effect, while the Meijiang watershed was examined to study the hydrological impact of reforestation. The key findings are: (1 both deforestation and reforestation led to significant reductions in dry season runoff, while climate variability yielded positive effects in the studied watersheds; (2 the hydrological response to forest change varied over time due to changes in soil infiltration and evapotranspiration after vegetation regeneration; (3 changes of subalpine natural forests produced greater impact on dry season runoff than alteration of planted forests. These findings are beneficial to water resource and forest management under climate change and highlight a better planning of forest operations and management incorporated trade-off between carbon and water in different forests.

  1. IM Based Speed Servodrive with Luenberger Observer

    Science.gov (United States)

    Gacho, Juraj; Žalman, Milan

    2010-05-01

    The article concerns observing states of the Induction Motor (IM) using a Luenberger observer in the speed servo drive. The movement of the motor and observer roots is analyzed for a variable speed. Following the analysis, a new method for gain evaluation of the IM magnetic flux observer is presented. This structure is extended by including an adaptive speed observer. The functionality of the presented method is proved in simulations using MATLAB Simulink.

  2. IG and TR single chain fragment variable (scFv) sequence analysis: a new advanced functionality of IMGT/V-QUEST and IMGT/HighV-QUEST.

    Science.gov (United States)

    Giudicelli, Véronique; Duroux, Patrice; Kossida, Sofia; Lefranc, Marie-Paule

    2017-06-26

    IMGT®, the international ImMunoGeneTics information system® ( http://www.imgt.org ), was created in 1989 in Montpellier, France (CNRS and Montpellier University) to manage the huge and complex diversity of the antigen receptors, and is at the origin of immunoinformatics, a science at the interface between immunogenetics and bioinformatics. Immunoglobulins (IG) or antibodies and T cell receptors (TR) are managed and described in the IMGT® databases and tools at the level of receptor, chain and domain. The analysis of the IG and TR variable (V) domain rearranged nucleotide sequences is performed by IMGT/V-QUEST (online since 1997, 50 sequences per batch) and, for next generation sequencing (NGS), by IMGT/HighV-QUEST, the high throughput version of IMGT/V-QUEST (portal begun in 2010, 500,000 sequences per batch). In vitro combinatorial libraries of engineered antibody single chain Fragment variable (scFv) which mimic the in vivo natural diversity of the immune adaptive responses are extensively screened for the discovery of novel antigen binding specificities. However the analysis of NGS full length scFv (~850 bp) represents a challenge as they contain two V domains connected by a linker and there is no tool for the analysis of two V domains in a single chain. The functionality "Analyis of single chain Fragment variable (scFv)" has been implemented in IMGT/V-QUEST and, for NGS, in IMGT/HighV-QUEST for the analysis of the two V domains of IG and TR scFv. It proceeds in five steps: search for a first closest V-REGION, full characterization of the first V-(D)-J-REGION, then search for a second V-REGION and full characterization of the second V-(D)-J-REGION, and finally linker delimitation. For each sequence or NGS read, positions of the 5'V-DOMAIN, linker and 3'V-DOMAIN in the scFv are provided in the 'V-orientated' sense. Each V-DOMAIN is fully characterized (gene identification, sequence description, junction analysis, characterization of mutations and amino

  3. Variable Myocardial Response to Load Stresses in Infants with Single Left Ventricular Anatomy: Influence of Initial Physiology and Surgical Palliative Strategy.

    Science.gov (United States)

    Horriat, Narges L; Deatsman, Sara L; Stelter, Jessica; Frommelt, Peter C; Hill, Garick D

    2016-12-01

    Initial surgical strategies in neonates with single left ventricular (LV) anatomy vary based on adequacy of pulmonary and systemic blood flow. Differing myocardial responses to these strategies, as reflected in indices of systolic function, ventricular size, and mass have not been well defined. We sought to evaluate single LV myocardial response to varied physiology and initial palliation and determine whether the response is consistent and predictable. Infants with single LV physiology were divided based on neonatal palliation: no palliation/PA band (NO); BT shunt only (BT); or Norwood procedure (NP). Echo measures were obtained at presentation, early post-bidirectional Glenn (BDG), late post-BDG follow-up, and post-Fontan procedure. Measures included ejection fraction, LV mass indexed to height(2.7) and end diastolic volume indexed to body surface area, and mass/volume ratio. The cohort included 38 children (13 NO, 13 BT, 12 NP). Ejection fraction was similar but depressed in all groups at all stages. LV mass was higher in the NP group than the BT group at early post-BDG (p = 0.03) and higher than both BT and NO groups (p < 0.01) at late post-BDG, but the difference was resolved by post-Fontan follow-up. The NP group had the most remarkable remodeling in LV size from BDG to Fontan, suggesting that volume unloading is most valuable in this subgroup. Ventricular remodeling can be identified by echocardiography in children with single LV physiology, despite variable initial surgical palliative strategies. Importantly, these initial surgical strategies do not result in significant differences after Fontan palliation during early childhood.

  4. High speed data converters

    CERN Document Server

    Ali, Ahmed MA

    2016-01-01

    This book covers high speed data converters from the perspective of a leading high speed ADC designer and architect, with a strong emphasis on high speed Nyquist A/D converters. For our purposes, the term 'high speed' is defined as sampling rates that are greater than 10 MS/s.

  5. Moderation of effects of AAC based on setting and types of aided AAC on outcome variables: an aggregate study of single-case research with individuals with ASD.

    Science.gov (United States)

    Ganz, Jennifer B; Rispoli, Mandy J; Mason, Rose Ann; Hong, Ee Rea

    2014-06-01

    The purpose of this meta-analysis was to evaluate the potential moderating effects of intervention setting and type of aided augmentative and alternative communication (AAC) on outcome variables for students with autism spectrum disorders. Improvement rate difference, an effect size measure, was used to calculate aggregate effects across 35 single-case research studies. Results indicated that the largest effects for aided AAC were observed in general education settings. With respect to communication outcomes, both speech generating devices (SGDs) and the Picture Exchange Communication System (PECS) were associated with larger effects than other picture-based systems. With respect to challenging behaviour outcomes, SGDs produced larger effects than PECS. This aggregate study highlights the importance of considering intervention setting, choice of AAC system and target outcomes when designing and planning an aided AAC intervention.

  6. A Single Sweep AGE Algorithm on a Variable Mesh Based on Off-Step Discretization for the Solution of Nonlinear Burgers’ Equation

    Directory of Open Access Journals (Sweden)

    R. K. Mohanty

    2014-01-01

    Full Text Available We discuss a new single sweep alternating group explicit iteration method, along with a third-order numerical method based on off-step discretization on a variable mesh to solve the nonlinear ordinary differential equation y′′=f(x,y,y′ subject to given natural boundary conditions. Using the proposed method, we have solved Burgers’ equation both in singular and nonsingular cases, which is the main attraction of our work. The convergence of the proposed method is discussed in detail. We compared the results of the proposed iteration method with the results of the corresponding double sweep alternating group explicit iteration methods to demonstrate computationally the efficiency of the proposed method.

  7. Evaluation of the effect of process variables on the fatty acid profile of single cell oil produced by Mortierella using solid-state fermentation.

    Science.gov (United States)

    Asadi, Seyedeh Zeinab; Khosravi-Darani, Kianoush; Nikoopour, Houshang; Bakhoda, Hossein

    2015-03-01

    This article reviews some of the aspects of single cell oil (SCO) production using solid-state fermentation (SSF) by fungi of the genus Mortierella. This article provides an overview of the advantages of SSF for SCO formation by the aforementioned fungus and demonstrates that the content of the polyunsaturated fatty acids (PUFA) depend on the type of fermentation media and culture conditions. Process variables that influence lipid accumulation by Mortierella spp. and the profile of the fatty acids are discussed, including incubation temperature, time, aeration, growth phase of the mycelium, particle size of the substrate, carbon to nitrogen ratio, initial moisture content and pH as well as supplementation of the substrate with nitrogen and oil. Finally, the article highlights future research trends for the scaled-up production of PUFAs in SSF.

  8. Single-chain variable fragment antibody against ginsenoside Re as an effective tool for the determination of ginsenosides in various ginsengs.

    Science.gov (United States)

    Pongkitwitoon, Benyakan; Sakamoto, Seiichi; Morinaga, Osamu; Juengwatanatrakul, Thaweesak; Shoyama, Yukihiro; Tanaka, Hiroyuki; Morimoto, Satoshi

    2011-01-01

    A single-chain variable fragment antibody (scFv) against ginsenoside Re (G-Re) was constructed and applied to an enzyme-linked immunosorbent assay (ELISA) for determining the total concentration of ginsenosides in various ginsengs. The variable heavy and light chain genes were cloned directly from the cDNA of the 4G10 hybridoma cell line and assembled by means of splicing by overlapping extension PCR (SOE-PCR) using specific primers designed to have flexible peptide (Gly(4)Ser)(3) between the variable heavy chain and light chain domains. The constructed scFv gene was ligated into the pET28a expression vector and transformed into E. coli BL21 (DE3). The recombinant scFv against G-Re (GRe-scFv) was expressed as a chimera protein containing the His6-tag at its N-termini, purified by immobilized metal ion affinity chromatography (IMAC), and refolded by a stepwise dialysis method. The yield of GRe-scFv after purification was 1.7 mg per liter of culture medium. Characterization of GRe-scFv revealed that it retained the characteristics of the parental monoclonal antibody (MAb) against G-Re (MAb-4G10) which has wide cross-reactivity with 20(S)-protopanaxadiol- and 20(S)-protopanaxatriol-type ginsenosides. The detectable range for G-Re in ELISA using scFv antibody was 0.02-10 µg/ml. Based on validation analysis, the use of GRe-scFv in ELISA is a precise, accurate, and sensitive method. In light of the time-consuming and labor-intensive procedures for the preparation of MAb, speedy bacterial expression of GRe-scFv is a powerful alternative tool for producing MAb to use in ELISA for quantitative analysis of total ginsenoside concentrations.

  9. Therapeutic anti-IgE monoclonal antibody single chain variable fragment (scFv) safety and immunomodulatory effects after one time injection in four dogs.

    Science.gov (United States)

    Hammerberg, Bruce; Eguiluz-Hernandez, Sitka

    2017-02-01

    The therapeutic monoclonal antibody omalizumab that is specific for IgE has proven to be an effective addition to the treatment of allergic disease in humans. The aims of this study were to demonstrate the safety and immunomodulating effects of a single injection of a monoclonal antibody single chain variable fragments (scFv) specific for canine IgE in normal dogs. Three normal dogs were bled for EDTA whole blood samples for 112 days post-injection (dpi). A fourth dog was monitored for 28 days. Anti-IgE scFv was pegylated to minimize scFv dimerization. Four normal dogs were injected once subcutaneously with anti-IgE scFv at 1 mg/kg. Flow cytometry was performed on whole blood. Plasma levels of IgE were measured by ELISA. None of the four dogs showed signs of anaphylaxis. All dogs demonstrated decreases in IgE(+) cells in lymphocyte-gated events by 14 dpi. Dogs C and D returned to pre-injection levels by 21 days, whereas dogs A and B remained below pre-injection levels until Day 112. Similar differences were seen in IgE-bearing granulocyte-gated cells. Free plasma IgE decreased below pre-injection levels by 47% in Dog A and by 52% in Dog B at 112 days. Dogs C and D did not change by more than 32% from preinjection levels. A single injection of monomeric, pegylated scFv with high affinity for dog IgE was demonstrated to be safe. Marked reduction in IgE-bearing lymphocytes and granulocytes accompanied by reduced "free" plasma IgE level in two of four dogs is analogous to omalizumab in humans. © 2016 ESVD and ACVD.

  10. AN APPLICATION OF MULTICRITERIA OPTIMIZATION TO THE TWO-CARRIER TWO-SPEED PLANETARY GEAR TRAINS

    Directory of Open Access Journals (Sweden)

    Jelena Stefanović-Marinović

    2017-04-01

    Full Text Available The objective of this study is the application of multi-criteria optimization to the two-carrier two-speed planetary gear trains. In order to determine mathematical model of multi-criteria optimization, variables, objective functions and conditions should be determined. The subject of the paper is two-carrier two-speed planetary gears with brakes on single shafts. Apart from the determination of the set of the Pareto optimal solutions, the weighted coefficient method for choosing an optimal solution from this set is also included in the mathematical model.

  11. An evaluation of the robustness of organ-at-risk recommendations made by GEC/ESTRO according to interobserver variability: a single-center experience

    Directory of Open Access Journals (Sweden)

    Rodolfo Chicas-Sett

    2016-08-01

    Full Text Available Purpose : Groupe Européen de Curiethérapie (GEC and European Society for Radiotherapy & Oncology (ESTRO has proposed a rectal dose constraint of the most exposed 2-cc volume (D 2cc of ≤ 75 Gy EQD 2α/β = 3 during external- beam plus high-dose-rate brachytherapy (HDR-BT in localized prostate cancer patients. This study aimed to evaluate D 2cc for rectal contouring via interobserver variability. Material and methods: Four blinded observers contoured rectums of 5 patients. Rectal contouring anatomical limits were determined through previous consensus. Dose-volume histogram (DVH dosimetric parameters (D 0.1cc , D 1cc , and D 2cc were analyzed according to GEC/ESTRO recommendations and subjected to intra- and interobserver comparisons. Latter comparisons involved coefficients of variation. For each parameter, the mean, standard deviation (SD, and range were evaluated. The effect of interobserver variation on total dose was analyzed by estimating the biologically equivalent rectal dose (EQD 2α/β = 3. Results : Interobserver coefficients of variation for D 0.1cc , D 1cc , and D 2cc were 5.7%, 4.5%, and 4%, respectively. The high­est interobserver rectal delineation variation yielded a rectal dose difference up to 5.8 Gy EQD2. Estimated intraobserver variation for the reported D 2cc was 5.5% in the worst-case scenario (non-significant. Conclusions : We observed acceptable interobserver variability in EQD2 for D 2cc , with strong impacts on clinical threshold levels (D 2cc ≤ 75 Gy EQD2 in some cases. This small, single-center analysis will be extended in a multicenter study.

  12. Expression and Functional Properties of an Anti-Triazophos High-Affinity Single-Chain Variable Fragment Antibody with Specific Lambda Light Chain

    Directory of Open Access Journals (Sweden)

    Rui Liu

    2016-06-01

    Full Text Available Triazophos is a widely used organophosphorous insecticide that has potentially adverse effects to organisms. In the present study, a high-affinity single-chain variable fragment (scFv antibody with specific lambda light chain was developed for residue monitoring. First, the specific variable regions were correctly amplified from a hybridoma cell line 8C10 that secreted monoclonal antibody (mAb against triazophos. The regions were then assembled as scFv via splicing by overlap extension polymerase chain reaction. Subsequently, the recombinant anti-triazophos scFv-8C10 was successfully expressed in Escherichia coli strain HB2151 in soluble form, purified through immobilized metal ion affinity chromatography, and verified via Western blot and peptide mass fingerprinting analyses. Afterward, an indirect competitive enzyme-linked immunosorbent assay was established based on the purified anti-triazophos scFv-8C10 antibody. The assay exhibited properties similar to those based on the parent mAb, with a high sensitivity (IC50 of 1.73 ng/mL to triazophos and no cross reaction for other organophosphorus pesticides; it was reliable in detecting triazophos residues in spiked water samples. Moreover, kinetic measurement using a surface plasmon resonance biosensor indicated that the purified scFv-8C10 antibody had a high affinity of 1.8 × 10−10 M and exhibited good binding stability. Results indicated that the recombinant high-affinity scFv-8C10 antibody was an effective detection material that would be promising for monitoring triazophos residues in environment samples.

  13. Speed management program plan.

    Science.gov (United States)

    2014-05-01

    Changing public attitudes regarding speeding and speed management will require a comprehensive and concerted effort, involving a wide variety of strategies. This plan identifies six primary focus areas: : A. Data and Data-Driven Approaches, : B. Rese...

  14. Characterization of a single-chain variable fragment recognizing a linear epitope of aβ: a biotechnical tool for studies on Alzheimer's disease?

    Directory of Open Access Journals (Sweden)

    Silke Dornieden

    Full Text Available Alzheimer's disease (AD is a progressive neurodegenerative disorder with devastating effects. Currently, therapeutic options are limited to symptomatic treatment. For more than a decade, research focused on immunotherapy for the causal treatment of AD. However, clinical trials with active immunization using Aβ encountered severe complications, for example meningoencephalitis. Consequently, attention focused on passive immunization using antibodies. As an alternative to large immunoglobulins (IgGs, Aβ binding single-chain variable fragments (scFvs were used for diagnostic and therapeutic research approaches. scFvs can be expressed in E. coli and may provide improved pharmacokinetic properties like increased blood-brain barrier permeability or reduced side-effects in vivo. In this study, we constructed an scFv from an Aβ binding IgG, designated IC16, which binds the N-terminal region of Aβ (Aβ(1-8. scFv-IC16 was expressed in E. coli, purified and characterized with respect to its interaction with different Aβ species and its influence on Aβ fibril formation. We were able to show that scFv-IC16 strongly influenced the aggregation behavior of Aβ and could be applied as an Aβ detection probe for plaque staining in the brains of transgenic AD model mice. The results indicate potential for therapy and diagnosis of AD.

  15. Expression of anti-tumor necrosis factor alpha (TNFα) single-chain variable fragment (scFv) in Spirodela punctata plants transformed with Agrobacterium tumefaciens.

    Science.gov (United States)

    Balaji, Parthasarathy; Satheeshkumar, P K; Venkataraman, Krishnan; Vijayalakshmi, M A

    2016-05-01

    Therapeutic antibodies against tumor necrosis factor alpha (TNFα) have been considered effective for some of the autoimmune diseases such as rheumatoid arthritis, Crohn's diseases, and so on. But associated limitations of the current therapeutics in terms of cost, availability, and immunogenicity have necessitated the need for alternative candidates. Single-chain variable fragment (scFv) can negate the limitations tagged with the anti-TNFα therapeutics to a greater extent. In the present study, Spirodela punctata plants were transformed with anti-TNFα through in planta transformation using Agrobacterium tumefaciens strain, EHA105. Instead of cefotaxime, garlic extract (1 mg/mL) was used to remove the agrobacterial cells after cocultivation. To the best of our knowledge, this report shows for the first time the application of plant extracts in transgenic plant development. 95% of the plants survived screening under hygromycin. ScFv cDNA integration in the plant genomic DNA was confirmed at the molecular level by PCR. The transgenic protein expression was followed up to 10 months. Expression of scFv was confirmed by immunodot blot. Protein expression levels of up to 6.3% of total soluble protein were observed. β-Glucuronidase and green fluorescent protein expressions were also detected in the antibiotic resistant plants. The paper shows the generation of transgenic Spirodela punctuata plants through in planta transformation. © 2015 International Union of Biochemistry and Molecular Biology, Inc.

  16. Characterization of the native and denatured herceptin by enzyme linked immunosorbent assay and quartz crystal microbalance using a high-affinity single chain fragment variable recombinant antibody.

    Science.gov (United States)

    Shang, Yuqin; Mernaugh, Ray; Zeng, Xiangqun

    2012-10-02

    Herceptin/Trastuzumab is a humanized IgG1κ light chain antibody used to treat some forms of breast cancer. A phage-displayed recombinant antibody library was used to obtain a single chain fragment variable (scFv, designated 2B4) to a linear synthetic peptide representing Herceptin's heavy chain CDR3. Enzyme linked immunosorbent assays (ELISAs) and piezoimmunosensor/quartz crystal microbalance (QCM) assays were used to characterize 2B4-binding activity to both native and heat denatured Herceptin. The 2B4 scFv specifically bound to heat denatured Herceptin in a concentration dependent manner over a wide (35-220.5 nM) dynamic range. Herceptin denatures and forms significant amounts of aggregates when heated. UV-vis characterization confirms that Herceptin forms aggregates as the temperature used to heat Herceptin increases. QCM affinity assay shows that binding stoichiometry between 2B4 scFv and Herceptin follows a 1:2 relationship proving that 2B4 scFv binds strongly to the dimers of heat denatured Herceptin aggregates and exhibits an affinity constant of 7.17 × 10(13) M(-2). The 2B4-based QCM assay was more sensitive than the corresponding ELISA. Combining QCM with ELISA can be used to more fully characterize nonspecific binding events in assays. The potential theoretical and clinical implications of these results and the advantages of the use of QCM to characterize human therapeutic antibodies in samples are also discussed.

  17. Speed in Acquisitions

    DEFF Research Database (Denmark)

    Meglio, Olimpia; King, David R.; Risberg, Annette

    2017-01-01

    The advantage of speed is often invoked by academics and practitioners as an essential condition during post-acquisition integration, frequently without consideration of the impact earlier decisions have on acquisition speed. In this article, we examine the role speed plays in acquisitions across...... the acquisition process using research organized around characteristics that display complexity with respect to acquisition speed. We incorporate existing research with a process perspective of acquisitions in order to present trade-offs, and consider the influence of both stakeholders and the pre......-deal-completion context on acquisition speed, as well as the organization’s capabilities to facilitating that speed. Observed trade-offs suggest both that acquisition speed often requires longer planning time before an acquisition and that associated decisions require managerial judgement. A framework for improving...

  18. Ship speed optimization: Concepts, models and combined speed-routing scenarios

    DEFF Research Database (Denmark)

    Psaraftis, Harilaos N.; Kontovas, Christos A.

    2014-01-01

    The purpose of this paper is to clarify some important issues as regards ship speed optimization at the operational level and develop models that optimize ship speed for a spectrum of routing scenarios in a single ship setting. The paper's main contribution is the incorporation of those fundament...

  19. From Romance to Rocket Science: Speed Dating in Higher Education

    Science.gov (United States)

    Muurlink, Olav; Poyatos Matas, Cristina

    2011-01-01

    This article is the first comprehensive review of speed dating in the tertiary sector. While speed dating has its origins as a networking technique to connect singles, it has only more recently made its way into the academy. Since 2005 universities world-wide have begun to adopt speed dating protocols as a tool for building research culture. An…

  20. Development of a single-chain variable fragment-alkaline phosphatase fusion protein and a sensitive direct competitive chemiluminescent enzyme immunoassay for detection of ractopamine in pork

    Energy Technology Data Exchange (ETDEWEB)

    Dong Jiexian; Li Zhenfeng; Lei Hongtao; Sun Yuanming [Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642 (China); Ducancel, Frederic [CEA, iBiTec-S, Service de Pharmacologie et d' Immnoanalyse (SPI), CEA Saclay, F-91191 Gif sur Yvette (France); Xu Zhenlin [Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642 (China); Boulain, Jean-Claude [CEA, iBiTec-S, Service de Pharmacologie et d' Immnoanalyse (SPI), CEA Saclay, F-91191 Gif sur Yvette (France); Yang Jinyi; Shen Yudong [Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642 (China); Wang Hong, E-mail: gzwhongd@63.com [Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642 (China)

    2012-07-29

    Graphical abstract: Detection model of dc-CLEIA based on anti-RAC scFv-AP fusion protein. Highlights: Black-Right-Pointing-Pointer The scFv-AP fusion protein against ractopamine (RAC) was produced. Black-Right-Pointing-Pointer A dc-CLEIA for RAC was developed based on the purified scFv-AP fusion protein. Black-Right-Pointing-Pointer The sensitivity of dc-CLEIA was 10 times as sensitive as dc-ELISA for RAC. Black-Right-Pointing-Pointer Recovery tests from pork samples were studied. Black-Right-Pointing-Pointer Good accuracy was obtained. - Abstract: A rapid, sensitive chemiluminescent enzyme immunoassay (CLEIA) for ractopamine (RAC) based on a single-chain variable fragment (scFv)-alkaline phosphatase (AP) fusion protein was developed. The scFv gene was prepared by cloning the heavy- and light-chain variable region genes (V{sub H} and V{sub L}) from hybridoma cell line AC2, which secretes antibodies against RAC, and assembling V{sub H} and V{sub L} genes with a linker by means of splicing overlap extension polymerase chain reaction. The resulting scFv gene was inserted into the expression vector pLIP6/GN containing AP to produce the fusion protein in Escherichia coli strain BL21. The purified scFv-AP fusion protein was used to develop a direct competitive CLEIA (dcCLEIA) protocol for detection of RAC. The average concentration required for 50% inhibition of binding and the limit of detection of the assay were 0.25 {+-} 0.03 and 0.02 {+-} 0.004 ng mL{sup -1}, respectively, and the linear response range extended from 0.05 to 1.45 ng mL{sup -1}. The assay was 10 times as sensitive as the corresponding enzyme-linked immunosorbent assay based on the same fusion protein. Cross-reactivity studies showed that the fusion protein did not cross react with RAC analogs. DcCLEIA was used to analyze RAC spiked pork samples, and the validation was confirmed by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS). The results showed a good correlation between