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Sample records for vana windowsiga arvutid

  1. Uue Vana Teatri 2. hooaeg

    Index Scriptorium Estoniae

    2003-01-01

    Uus Vana Teater mängib sel hooajal Meistrite Hoovi uues teatrisaalis. Hakatakse ette valmistama H. von Bingeni müsteeriumi "Vooruste mäng" ja V. Huovise "Havukkaaho mõtlejat". Samuti on kavas teha improvisatsiooniteatrit

  2. Tuulelipp Vana Toomas = Weathervane Vana Toomas / Elvira Liiver Holmström

    Index Scriptorium Estoniae

    Liiver Holmström, Elvira

    2004-01-01

    1530. a. parandas Riiast pärit torniehitaja Kersten Pelser Tallinna Raekoja torni ja seadis selle tippu nüüd Vana Toomana tuntud sõjasulase figuuriga tuulelipu. 1944. a. hävinud tornikiiver taastati, vaskmehe asemele pandi Vana Toomas II. 1996. a. paigaldati uus tornikiiver koos Vana Toomas III-ga

  3. Prantsuse kunstnikud asustasid vana tehase / Johannes Saar

    Index Scriptorium Estoniae

    Saar, Johannes, 1965-

    2002-01-01

    Prantsuse kunstnike (Delphine Reist, Laurent Faulon, Sebastien Perroud) ja viiuldaja Mathieu Werchowsky Tallinnas valminud installatsiooninäitus "Neli laulu Siivile" vanas Kalinini tehasehoones 7.-12. juunini

  4. Kultuuritehas vallutab vana katlamaja / Merit Kask

    Index Scriptorium Estoniae

    Kask, Merit

    2005-01-01

    Kultuuritehase projektijuht Madis Mikkor Tallinna Põhja puiesteel asuva vana elektrijaama tööstusmiljööst ja hoonetest, kuhu kolib kultuuritehas Polymer. Katlamaja plaanitakse avada augustis mitmepäevase festivaliga

  5. Raimo Pullati XX "Vana Tallinn" / Tõnis Liibek

    Index Scriptorium Estoniae

    Liibek, Tõnis

    2009-01-01

    Ilmus professor Raimo Pullati poolt välja antava linnaajalugu ja -kultuuri tutvustava sarja "Vana Tallinn" XX köide. Tšehhi Teaduste Akadeemia raamatukogus toimus Raimo Pullati poolt välja antud entsüklopeedilise suurteose "Johann Christoph Brotze. Estonica" esitlus

  6. 11-keelset "Vana Kannelt" sõrmitsemas / Helen Kõmmus

    Index Scriptorium Estoniae

    Kõmmus, Helen, 1975-

    2014-01-01

    Arvustus: Kodavere regilaulud / Eesti Kirjandusmuuseum, Eesti Rahvaluule Arhiiv ; koostajad Liina Saarlo, Edna Tuvi. Tartu : Eesti Kirjandusmuuseum, 2014. (Monumenta Estoniae antiquae. I, Vana kannel ; 11)

  7. Determination of vehicular pollution in the road tunnel of Vana ...

    African Journals Online (AJOL)

    Determination of vehicular pollution in the road tunnel of Vana (Haraz Road) in the ... source of pollution in a road tunnel, It has a harmful impact on Human health . ... undoubtedly one of the important sources of environmental and air pollution.

  8. Vana-Võidu mõisas avastati Herculaneumi maalingute koopiad / Hilkka Hiiop

    Index Scriptorium Estoniae

    Hiiop, Hilkka, 1974-

    2016-01-01

    2016. aasta jaanuaris leiti Vana-Võidu mõisahoones maalingud, mis pärinevad mõisa ehitusajast 19. sajandi keskpaigast. Need on Vana-Rooma suvituslinnast Herculaneumist leitud maalingute tippkvaliteediga koopiad

  9. DVD. Aare Ermel soovitab : "Vana daami visiit" / Aare Ermel

    Index Scriptorium Estoniae

    Ermel, Aare, 1957-2013

    2006-01-01

    ETV egiidi all valminud mängufilm Ita Everi juubeliks Friedrich Dürrenmatti näidendi järgi "Vana daami visiit" : režissöör Roman Baskin. ETV DVD-l on lisana R. Baskini dokfilm "Ita Everi portree"

  10. Vana Baskini teater mängib suvelavastust / Andris Tammela

    Index Scriptorium Estoniae

    Tammela, Andris

    2009-01-01

    Pärnu Kalevi staadionil ja Kilingi-Nõmme suveaias etenduvast Vana Baskini teatri suvekomöödiast "Hullem kui Hollywoodis". John Patricku näidendi lavastas Ivo Eensalu ja kujundas Kustav-Agu Püüman. Peaosas Helgi Sallo

  11. Õigus Vanas Testamendis : [bakalaureusetöö] / Haide Rimmel ; Tartu Ülikool, õigusteaduskond ; juhendaja: Peeter Järvelaid

    Index Scriptorium Estoniae

    Rimmel, Haide

    1996-01-01

    Vana Testamendi õiguse üldiseloomustus (kasuistlikud normid, apodiktilised normid), Vana Testamendi õiguse allikad (lepinguraamat, deuteronoomium, Pühadusseadus ja Preestrikoodeks, muud allikad), Vana Testamendi õiguse subjektid (täieõiguslikud kodanikud, võõramaalased, orjad), Vana Testamendi õigusharud (kriminaalõigus, perekonnaõigus, pärimisõigus, võlaõigus, asjaõigus), õigusemõistmine Vanas Testamendis

  12. Sisaliku jäljed piiblitõlkes : lõunaeestikeelse Vana Testamendi saatusest / Kristiina Ross

    Index Scriptorium Estoniae

    Ross, Kristiina, 1955-

    2011-01-01

    Uuritakse Kambja õpetaja Heinrich Anreas Erxlebeni lõunaeestikeelse Vana Testamendi tõlget (1796) ning leitakse, et sõnastuslikult langeb see suures osas kokku sada aastat varasema Andreas ja Adrian Virginiuse põhjaeestikeelse käsikirjaga

  13. Polyclonal emergence of vanA vancomycin-resistant Enterococcus faecium in Australia.

    Science.gov (United States)

    van Hal, Sebastiaan J; Espedido, Björn A; Coombs, Geoffrey W; Howden, Benjamin P; Korman, Tony M; Nimmo, Graeme R; Gosbell, Iain B; Jensen, Slade O

    2017-04-01

    To investigate the genetic context associated with the emergence of vanA VRE in Australia. The whole genomes of 18 randomly selected vanA -positive Enterococcus faecium patient isolates, collected between 2011 and 2013 from hospitals in four Australian capitals, were sequenced and analysed. In silico typing and transposon/plasmid assembly revealed that the sequenced isolates represented (in most cases) different hospital-adapted STs and were associated with a variety of different Tn 1546 variants and plasmid backbone structures. The recent emergence of vanA VRE in Australia was polyclonal and not associated with the dissemination of a single 'dominant' ST or vanA -encoding plasmid. Interestingly, the factors contributing to this epidemiological change are not known and future studies may need to consider investigation of potential community sources.

  14. 'Vana' ja 'moodne' ehituskultuuris / Jaak Poom ; tõlk. Peeter Tammisto

    Index Scriptorium Estoniae

    Poom, Jaak

    1998-01-01

    Vanad majad põlu all I maailmasõja järel. Modernism arhitektuuris ja linnaehituses pärast II maailmasõda. Funktsionalismi tagaküljed. Jälle hinda tõusnud vanade hoonete säilitamise tehnilised ja majanduslikud aspektid. Arhitekt ja 'vana' säilitamine. Vana tähendus täna. Ill.: uus ärikvartal Kyrkolunden Sigtuna vanalinnas

  15. Emergence of vanA Enterococcus faecium in Denmark, 2005-15

    DEFF Research Database (Denmark)

    Hammerum, Anette M; Baig, Sharmin; Kamel, Yasmin

    2017-01-01

    Objectives: To describe the changing epidemiology of vancomycin-resistant Enterococcus faecium and Enterococcus faecalis in clinical samples in Denmark 2005-15 according to species and van type, and, furthermore, to investigate the genetic relatedness of the clinical E. faecium isolates from 2015...... for the vancomycin-resistant E. faecium isolates. Results: During 2005-15, 1043 vanA E. faecium , 25 vanB E. faecium , 4 vanA E. faecalis and 28 vanB E. faecalis were detected. The number of VRE was  200 isolates/year in 2013-15. In 2015, 368 vanA E. faecium and 1 vanB E. faecium...... were detected along with 1 vanA E. faecalis and 1 vanB E. faecalis . cgMLST subdivided the 368 vanA E. faecium isolates into 33 cluster types (CTs), whereas the vanB E. faecium isolate belonged to a different CT. ST203-CT859 was most prevalent (51%), followed by ST80-CT14 (22%), ST117-CT24 (6%), ST80...

  16. Muinas-Hellase ja Vana-Egiptuse kultuuriseosed Gustav Teichmülleri nägemuses / Sergei Stadnikov

    Index Scriptorium Estoniae

    Stadnikov, Sergei, 1956-

    2007-01-01

    Vana-Kreeka filosoofia ja vana-egiptuse teoloogia seotusest Teichmülleri käsitluses, mis oli erandlik ja omanäoline, silmas pidades 19. saj. 70ndate aastate ideoloogilist situatsiooni saksa humnitaarteaduses. Herakleitosest kui ida-lääne kommunikatsiooni esindajast.

  17. Emergence of vanA Enterococcus faecium in Denmark, 2005-15.

    Science.gov (United States)

    Hammerum, Anette M; Baig, Sharmin; Kamel, Yasmin; Roer, Louise; Pinholt, Mette; Gumpert, Heidi; Holzknecht, Barbara; Røder, Bent; Justesen, Ulrik S; Samulioniené, Jurgita; Kjærsgaard, Mona; Østergaard, Claus; Holm, Anette; Dzajic, Esad; Søndergaard, Turid Snekloth; Gaini, Shahin; Edquist, Petra; Alm, Erik; Lilje, Berit; Westh, Henrik; Stegger, Marc; Hasman, Henrik

    2017-08-01

    To describe the changing epidemiology of vancomycin-resistant Enterococcus faecium and Enterococcus faecalis in clinical samples in Denmark 2005-15 according to species and van type, and, furthermore, to investigate the genetic relatedness of the clinical E. faecium isolates from 2015. During 2005-14, all clinical VRE isolates were tested for the presence of vanA/B/C genes by PCR. In 2015, all clinical VRE isolates were whole-genome sequenced. From the WGS data, the presence of van genes and MLST STs were extracted in silico . Core-genome MLST (cgMLST) analysis was performed for the vancomycin-resistant E. faecium isolates. During 2005-15, 1043 vanA E. faecium , 25 vanB E. faecium , 4 vanA E. faecalis and 28 vanB E. faecalis were detected. The number of VRE was  200 isolates/year in 2013-15. In 2015, 368 vanA E. faecium and 1 vanB E. faecium were detected along with 1 vanA E. faecalis and 1 vanB E. faecalis . cgMLST subdivided the 368 vanA E. faecium isolates into 33 cluster types (CTs), whereas the vanB E. faecium isolate belonged to a different CT. ST203-CT859 was most prevalent (51%), followed by ST80-CT14 (22%), ST117-CT24 (6%), ST80-CT866 (4%) and ST80-CT860 (2%). Comparison with the cgMLST.org database, previous studies and personal communications with neighbouring countries revealed that the novel cluster ST203-CT859 emerged in December 2014 and spread to the south of Sweden and the Faroe Islands during 2015. VRE increased in Denmark during 2005-15 due to the emergence of several vanA E. faecium clones.

  18. Järgsilpide "o" ja "u" varieerumine ning traditsioonid vanas kirjakeeles / Külli Prillop

    Index Scriptorium Estoniae

    Prillop, Külli

    2009-01-01

    Kirjeldatakse, mida võib vanade tekstide põhjal oletada "o" ja "u" vaheldumisest ja selle põhjustest, kasutades eelkõige Georg Mülleri jutluste tekste. Selgitatakse ka probleeme vana kirjakeele tekstide põhjal keelemuutuste kohta järelduste tegemisel

  19. Käivad Ita Everi juubelifilmi "Vana daami visiit" võtted / Andris Feldmanis

    Index Scriptorium Estoniae

    Feldmanis, Andris, 1982-

    2006-01-01

    Mängufilm "Vana daami visiit" : stsenaristid Roman Baskin, Ilmar Raag : režissöör Roman Baskin : peaosas Ita Ever, kaasa lööb üle 30 tuntud eesti näitleja : MTÜ Kell Kümme koostöös ETV ja Exitfilmiga, toetab ka Kultuurkapital

  20. Millised piirid nähtuvad läänemeresoome vanasõnades? / Arvo Krikmann

    Index Scriptorium Estoniae

    Krikmann, Arvo, 1939-2017

    1997-01-01

    Soome Kirjanduse Seltsi ja Eesti Teaduste Akadeemia vahelise 1993. aasta leppega käivitatud läänemeresoome vanasõnaprojekti tulemustest. Valminud töödest, pikemalt: Arvo Krikmann. Some statistics on Balto-Finnic proverbs (1985)

  1. Host range of enterococcal vanA plasmids among Gram-positive intestinal bacteria

    DEFF Research Database (Denmark)

    Werner, Guido; Freitas, Ana R.; Coque, Teresa M.

    2010-01-01

    OBJECTIVES: The most prevalent type of acquired glycopeptide resistance is encoded by the vanA transposon Tn1546 located mainly on transferable plasmids in Enterococcus faecium. The limited occurrence in other species could be due to the lack of inter-species transferability and/or stability of T...

  2. Arco Vara nõus Kodukolde kliendi vana korteri ostma / Kadi Heinsalu

    Index Scriptorium Estoniae

    Heinsalu, Kadi, 1966-

    2007-01-01

    Ilmunud ka: Delovõje Vedomosti 24. okt. lk. 29. Arco Vara on valmis ära ostma kliendi vana korteri ja andma pangale ostja eest garantii 15protsendilisele omaosalusele. Vt. samas: Arakas: nagu autovahetus kümne aasta eest. Kommenteerivad Monica Meldo ja Olev Mait Makk

  3. Molecular analysis of diverse elements mediating VanA glycopeptide resistance in enterococci

    DEFF Research Database (Denmark)

    Palepou, M.F.I.; Adebiyi, A.M.A.; Tremlett, C.H.

    1998-01-01

    . All elements had conserved vanRSHAX genes, but variation occurred upstream of vanR and downstream of vanX. Twenty-one VanA elements had significant alterations upstream of vanR in the transposition genes orf1 and orf2: either parts of these genes were absent or they were disrupted by IS1216V or IS3...

  4. Rotermanni vana ja uus jahuladu = The Rotermann Old and New Flour Storage / Margit Mutso

    Index Scriptorium Estoniae

    Mutso, Margit, 1966-

    2010-01-01

    Tallinna Rotermanni 8 asuvast Rotermanni uuest ja vanast jahulaost, mis pälvis 2009. a. EK arhitektuuri sihtkapitali arhitektuuripreemia linnaruumi tunnetava, uut ja vana tervikuks siduva arhitektuuri eest. Arhitektid: Hanno Grossschmidt, Tomomi Hayashi, Yoko Azukawa (HG Arhitektuur). Büroode üldkujundus: Tarmo Piirmets, Raul Tiitus (OÜ Pink). Žürii liige Kalle Komissarov žürii hinnangust hoonele

  5. Grammatiseerumise ja leksikaliseerumise suhtest eesti vana kirjakeele modaalpartiklite näitel / Külli Habicht

    Index Scriptorium Estoniae

    Habicht, Külli, 1964-

    2006-01-01

    Uuritakse grammatiseerumist ja leksikaliseerumist ja püütakse jõuda jäeldusele, kas ainult nendega on võimalik hõlmata igasuguste keeleüksuste kujunemise protsessi. Näidetena kasutatakse vana kirjakeele modaalpartikleid

  6. Jõujooned ristusid vanas lennukitehases / Peetr Ora, Taago Tubin ; intervjueerinud Margus Haav

    Index Scriptorium Estoniae

    Ora, Peeter

    2009-01-01

    Ugala teater lavastab koostöös MTÜ Kultuuritsehhiga Viljandi vanas lennukitehases Rootsi kirjaniku Lotta Lotassi näidendi "Kogujad". Lavastaja Taago Tubina ja kunstnik Peeter Oraga uuest mängupaigast, näidendist, blogist Twitteris ja lavastuse sissejuhatavatest õhtutest

  7. Co-colonization of vanA and vanB Enterococcus faecium of clonal complex 17 in a patient with bacteremia due to vanA E. faecium.

    Science.gov (United States)

    Seol, Chang Ahn; Park, Jeong Su; Sung, Heungsup; Kim, Mi-Na

    2014-06-01

    A 53-year-old Vietnamese man with liver cirrhosis was transferred from a Vietnamese hospital to our tertiary care hospital in Korea in order to undergo a liver transplantation. Bacteremia due to vanA Enterococcus faecium was diagnosed, and stool surveillance cultures for vancomycin-resistant enterococci (VRE) were positive for both vanA and vanB E. faecium. Pulsed-field gel electrophoresis analysis revealed that the 2 vanA VRE isolates from the blood and stool were clonal, but the vanB VRE was unrelated to the vanA VRE. vanA and vanB VRE were ST64 and ST18, single-allele variations of clonal complex 17, respectively. This is the first case report of vanA VRE bacteremia in a Vietnamese patient and demonstrates the reemergence of vanB VRE since a single outbreak occurred 15years ago in Korea. The reemergence of vanB VRE emphasizes the importance of VRE genotyping to prevent the spread of new VRE strains. Copyright © 2014 Elsevier Inc. All rights reserved.

  8. Enterococcus gallinarum carrying the vanA gene cluster: first report in Brazil

    Directory of Open Access Journals (Sweden)

    Camargo I.L.B.C.

    2004-01-01

    Full Text Available In 2000, Enterococcus faecalis resistant to vancomycin was first reported at a tertiary hospital in Porto Alegre, southern Brazil. The resistance spread to other hospitals and surveillance programs were established by hospital infection committees to prevent the spread of vancomycin-resistant enterococci. In February 2002, an isolate initially identified at the genus level as Enterococcus was obtained by surveillance culture (rectal swab from a patient admitted to a hospital for treatment of septic arthritis in the shoulder. The isolate proved to be resistant to vancomycin by the disc diffusion method and confirmed by an E-test resulting in a minimal inhibitory concentration of > or = 256 µg/ml. This isolate was sent to a reference laboratory (Laboratório Especial de Bacteriologia e Epidemiologia Molecular, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, USP for further study and proved to be an E. gallinarum by the polymerase chain reaction (PCR using specific primers for the species. Due to the phenotype of unusually high vancomycin resistance, the isolate presumably had the resistance genes (vanA and vanB and this was confirmed by PCR, which indicated the presence of the vanA gene. A 10.8-kb Tn1546-related transposon was also identified by long-PCR. Interspecies transfer of the vancomycin-resistance gene from the donor E. gallinarum was performed in a successful conjugation experiment in vitro, using E. faecium GE-1 and E. faecalis JH22 as receptors. This is the first report of the detection of a vanA determinant naturally acquired by E. gallinarum in Brazil, indicating the importance of characterizing VRE by both phenotype and genotype methods.

  9. Enterococcus gallinarum carrying the vanA gene cluster: first report in Brazil.

    Science.gov (United States)

    Camargo, I L B C; Barth, A L; Pilger, K; Seligman, B G S; Machado, A R L; Darini, A L C

    2004-11-01

    In 2000, Enterococcus faecalis resistant to vancomycin was first reported at a tertiary hospital in Porto Alegre, southern Brazil. The resistance spread to other hospitals and surveillance programs were established by hospital infection committees to prevent the spread of vancomycin-resistant enterococci. In February 2002, an isolate initially identified at the genus level as Enterococcus was obtained by surveillance culture (rectal swab) from a patient admitted to a hospital for treatment of septic arthritis in the shoulder. The isolate proved to be resistant to vancomycin by the disc diffusion method and confirmed by an E-test resulting in a minimal inhibitory concentration of > or = 256 microg/ml. This isolate was sent to a reference laboratory (Laboratorio Especial de Bacteriologia e Epidemiologia Molecular, Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, USP) for further study and proved to be an E. gallinarum by the polymerase chain reaction (PCR) using specific primers for the species. Due to the phenotype of unusually high vancomycin resistance, the isolate presumably had the resistance genes (vanA and vanB) and this was confirmed by PCR, which indicated the presence of the vanA gene. A 10.8-kb Tn1546-related transposon was also identified by long-PCR. Interspecies transfer of the vancomycin-resistance gene from the donor E. gallinarum was performed in a successful conjugation experiment in vitro, using E. faecium GE-1 and E. faecalis JH22 as receptors. This is the first report of the detection of a vanA determinant naturally acquired by E. gallinarum in Brazil, indicating the importance of characterizing VRE by both phenotype and genotype methods.

  10. Genes homologous to glycopeptide resistance vanA are widespread in soil microbial communities

    DEFF Research Database (Denmark)

    Guardabassi, L.; Agersø, Yvonne

    2006-01-01

    The occurrence of D-Ala : D-Lac ligase genes homologous to glycopeptide resistance vanA was studied in samples of agricultural (n=9) and garden (n=3) soil by culture-independent methods. Cloning and sequencing of nested degenerate PCR products obtained from soil DNA revealed the occurrence of D......A in enterococci. Such sequences were recovered from all agricultural samples as well as from two garden samples with no history of organic fertilization. The results indicated that soil is a rich and assorted reservoir of genes closely related to those conferring glycopeptide resistance in clinical bacteria....

  11. Uudised : Pärdi festival Salzburgis. Sumera uus teos. Toomas Vana edu Macaus. Vanemuise muusikajuht-peadirigent / Jaanika Juhanson

    Index Scriptorium Estoniae

    Juhanson, Jaanika, 1977-

    1999-01-01

    T. Kaljuste ja EFK esitasid Salzburgis Pärdi festivalil teose "Kanon pokajanen". Haagis toimub L. Sumera tshellokontserdi maailmaesiettekanne. T. Vana sai Macaus toimunud pianistide konkursil "Vianna da Motta" IV koha. Vanemuise uueks muusikajuhiks ja peadirigendiks valiti M. Kütson

  12. Kiviõli vana tuhamäe all võib olla koonduslaagri ohvrite matmispaiku / Gerli Romanovitsh

    Index Scriptorium Estoniae

    Romanovitš, Gerli, 1977-

    2008-01-01

    Sihtasutus Kiviõli Seiklusturismi Keskus uurib vangi- ja koonduslaagri ohvrite võimalikke matmispaiku vana poolkoksimäe ümbruses. Ilmunud ka: Severnoje Poberezhje, 26. juuli 2008 (Pod starõm kiviõliskim zolootvalom mogut okazatsja zahhoronenija zhertv kontslagerja)

  13. American crows as carriers of vancomycin-resistant enterococci with vanA gene.

    Science.gov (United States)

    Oravcova, Veronika; Zurek, Ludek; Townsend, Andrea; Clark, Anne B; Ellis, Julie C; Cizek, Alois; Literak, Ivan

    2014-04-01

    We studied the vanA-carrying vancomycin-resistant enterococci (VRE) isolated from American crows in the United States during the winter 2011/2012. Faecal samples from crows were cultured selectively for VRE and characterized. Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) were used to examine epidemiological relationships of vanA-containing VRE. Isolates were tested in vitro for their ability to horizontally transfer the vancomycin resistance trait. VRE with the vanA gene were found in 15 (2.5%) of 590 crows samples, from which we obtained 22 different isolates. Enterococcal species were Enterococcus faecium (14) and E. faecalis (8). One, two and 19 isolates originated from Kansas, New York State and Massachusetts, respectively. Based on MLST analysis, E. faecium isolates were grouped as ST18 (6 isolates), ST555 (2), and novel types ST749 (1), ST750 (3), ST751 (1), ST752 (1). Enterococcus faecalis isolates belonged to ST6 (1), ST16 (3) and ST179 (4). All isolates were able to transfer the vancomycin resistance trait via filter mating with very high transfer range. Clinically important enterococci with the vanA gene occur in faeces of wild American crows throughout the United States. These migrating birds may contribute to the dissemination of VRE in environment over large distances. [Correction added after first online publication on 06 August 2013: The number of E. faecium ST752 isolate is now amended to '1', consistent with that shown in the 'Results' section and Figure 2.]. © 2013 Society for Applied Microbiology and John Wiley & Sons Ltd.

  14. Sõnaliik kui rakenduslik probleem : sõnaliikide märgendamine vana kirjakeele korpuses / Külli Habicht, Pille Penjam, Külli Prillop

    Index Scriptorium Estoniae

    Habicht, Külli, 1964-

    2011-01-01

    Käsitletakse sõnade liigitamisega seotud probleeme eesti vana kirjakeele korpuse märgendamise praktikas. Tutvustatakse ka kasutusel olevat märgendussüsteemi ning raskusi selle tegelikul rakendamisel

  15. Põhja-Euroopa ja Vana Iisrael : Udmurdi ja Põhja-Euroopa paralleel lepingulaeka rändamisele muistses Iisraelis / Uku Masing ; [tõlkinud Kalle Kasemaa

    Index Scriptorium Estoniae

    Masing, Uku, 1909-1985

    2005-01-01

    Vanas Testamendis kirjeldatud lepingulaeka rändamisele sarnast tava on kirjeldatud udmurtidel. Muistsete saagade põhjal võib oletada, et loomade abil pühamu koha leidmise komme esines ka kristluse-eelses Põhja-Euroopas

  16. VanA and VanB Positive Vancomycin-resistant Staphylococcus aureus Among Clinical Isolates in Shiraz, South of Iran

    Directory of Open Access Journals (Sweden)

    Sareh Saadat

    2014-09-01

    Full Text Available Objective: The purpose of this study was to determine the prevalence of vancomycin-resistant Staphylococcus aureus isolated from clinical samples in Shiraz hospitals. Methods: From March to December 2012, 100 S. aureus isolates (mainly from wound and blood were collected from three hospitals in Shiraz, south of Iran. After identification of Staphylococcus aureus by biochemical, microbiological and molecular methods, antibiotic susceptibility testing was performed by Kirby-Bauer disc diffusion test for 13 different antibiotics. Vancomycin-resistant Staphylococcus aureus isolates were determined by vancomycin agar screening test and PCR for vancomycin resistant genes (vanA and vanB. Results: The lowest and highest resistance was seen for quinupristin-dalfopristin (n=1 and ampicillin (n=95, respectively. Vancomycin agar screening test showed that 37 isolates can grow on these media. Further study by PCR also detected vanA and/or vanB genes in all of these strains. Also, 19 isolates showed either vanA or vanB but were susceptible according to vancomycin agar screening test. In total, vanA and vanB resistant genes were detected in 34% and 37% of clinical isolates, respectively. Conclusion: The results showed that the frequency of vancomycin resistance genes (vanA, vanB is very high in Staphylococcus aureus strains isolated from patients in south of Iran. Thus, urgent interventions are needed to keep the emergence and transmission of these isolates to a minimum.

  17. vanA Gene Harboring Enterococcal and Non-enterococcal Isolates Expressing High Level Vancomycin and Teicoplanin Resistance Reservoired in Surface Waters.

    Science.gov (United States)

    Nakipoğlu, Mustafa; Yilmaz, Fadime; Icgen, Bulent

    2017-05-01

    Untreated wastewaters and treated effluents even after final disinfection contain antibiotic resistant bacteria and resistance genes before they are released into surface waters. A correlation between resistant bacteria and antibiotics in surface waters has been found, as have antibiotic resistance genes. Of particular interest are vancomycin-resistant enterococci harboring vanA gene that confers high level of resistance to glycopeptide antibiotics including teicoplanin. Therefore, in this study, river water samples were analysed to investigate vancomycin- and teicoplanin-resistant bacterial isolates harboring vanA gene. Out of 290, 15 surface water isolates displayed resistance to both antibiotics. These glycopeptide resistant enterococcal and non-enterococcal isolates, identified by 16S rRNA sequencing, were found to harbor vanA gene with sequence similarities of 50 % to 100 %. The presence of D-alanine-D-lactate ligase encoded by vanA gene was also shown for all vancomycin- and teicoplanin-resistant isolates through western blot analysis. Due to reuse of treated wastewater and release of untreated wastewaters to water bodies, antibiotic resistant bacteria and resistance genes are being introduced into surface waters and present human health risks. Therefore, surface waters are not only hot spots for vanA harboring enterococcal isolates but also non-enterococcal isolates due to gene dissemination and require special scientific consideration.

  18. First description in Europe of the emergence of Enterococcus faecium ST117 carrying both vanA and vanB genes, isolated in Greece.

    Science.gov (United States)

    Papagiannitsis, C C; Malli, E; Florou, Z; Medvecky, M; Sarrou, S; Hrabak, J; Petinaki, E

    2017-12-01

    An Enterococcus faecium isolate (Efa-125) carrying both the vanA and vanB genes was recovered from a patient with bacteraemia treated in a Greek hospital. Since this is the first description in Europe of E. faecium carrying both vanA and vanB genes, the isolate was further studied. Susceptibility to several antibiotics was determined using the VITEK ® 2 automated system. The isolate was typed by multilocus sequence typing (MLST). To define the genetic units of the vanA and vanB genes, the plasmid content of Efa-125 was analysed by pulsed-field gel electrophoresis (PFGE) of total DNA digested with S1 nuclease followed by hybridisation with digoxigenin-labelled vanA and vanB probes. In addition, plasmids and chromosomes were sequenced using the Illumina MiSeq platform. E. faecium Efa-125 belonged to ST117 and expressed resistance both to vancomycin and teicoplanin, with minimum inhibitory concentrations (MICs) for both of 256mg/L. The vanA gene was carried on a 29 320-bp plasmid exhibiting high similarity to pA6981 previously characterised from Enterococcus gallinarum A6981, whereas vanB was part of a Tn1549-like transposon integrated into the chromosome. Expression of the VanA phenotype was correlated with the presence of intact vanZ and vanS genes. This is the first detection in Greece of vanA-vanB genotype/VanA phenotype E. faecium and indicates an evolving epidemiology of vancomycin-resistant enterococci. Copyright © 2017 International Society for Chemotherapy of Infection and Cancer. Published by Elsevier Ltd. All rights reserved.

  19. Enterococcus faecalis resistant to vancomycin and teicoplanin (VanA phenotype isolated from a bone marrow transplanted patient in Brazil

    Directory of Open Access Journals (Sweden)

    Rosangela F. Cereda

    Full Text Available We report for the first time in Brazil, a patient from whom an Enterococcus faecalis VanA phenotype was isolated. Glycopeptide resistance is not commonly observed in Enterococcus faecalis, so this finding is of great concern since this species is responsible for 90% of enterococcal infections in Brazil. The isolate was recovered from a surveillance rectal swab culture from a patient with acute lymphocytic leukemia (ALL. Identification to the species level was performed by conventional biochemical tests and Vitek GPI cards. Antimicrobial susceptibility testing was evaluated by use of broth microdilution and Etest (AB BIODISK, Solna, Sweden methods. The isolate was identified as E. faecalis and was considered resistant to both vancomycin (MIC, > 256 mug/mL and teicoplanin (MIC, 256 mug/mL. The isolate also showed high level resistance to gentamicin and streptomycin (MICs, > 1024 mug/mL, but was considered susceptible to ampicillin (MIC, 4 mug/mL. Although the frequency of enterococcal infections is very low in most Latin America countries, the finding of glycopeptide (VanA resistance in E. faecalis increases concern about apreading antimicrobial resistance in this region.

  20. Enterococcus faecalis resistant to vancomycin and teicoplanin (VanA phenotype) isolated from a bone marrow transplanted patient in Brazil.

    Science.gov (United States)

    Cereda, R; Sader, H; Sejas, L; Machado, A; Zanatta, Y; Rego, S; Medeiros, E

    2001-02-01

    We report for the first time in Brazil, a patient from whom an Enterococcus faecalis VanA phenotype was isolated. Glycopeptide resistance is not commonly observed in Enterococcus faecalis, so this finding is of great concern since this species is responsible for 90% of enterococcal infections in Brazil. The isolate was recovered from a surveillance rectal swab culture from a patient with acute lymphocytic leukemia (ALL). Identification to the species level was performed by conventional biochemical tests and Vitek GPI cards. Antimicrobial susceptibility testing was evaluated by use of broth microdilution and Etest (AB BIODISK, Solna, Sweden) methods. The isolate was identified as E. faecalis and was considered resistant to both vancomycin (MIC, > 256 microg/mL) and teicoplanin (MIC, 256 microg/mL). The isolate also showed high level resistance to gentamicin and streptomycin (MICs, > 1024 microg/mL), but was considered susceptible to ampicillin (MIC, 4 microg/mL). Although the frequency of enterococcal infections is very low in most Latin America countries, the finding of glycopeptide (VanA) resistance in E. faecalis increases concern about apreading antimicrobial resistance in this region.

  1. Enterococcus faecalis resistant to vancomycin and teicoplanin (VanA phenotype isolated from a bone marrow transplanted patient in Brazil

    Directory of Open Access Journals (Sweden)

    Cereda Rosangela F.

    2001-01-01

    Full Text Available We report for the first time in Brazil, a patient from whom an Enterococcus faecalis VanA phenotype was isolated. Glycopeptide resistance is not commonly observed in Enterococcus faecalis, so this finding is of great concern since this species is responsible for 90% of enterococcal infections in Brazil. The isolate was recovered from a surveillance rectal swab culture from a patient with acute lymphocytic leukemia (ALL. Identification to the species level was performed by conventional biochemical tests and Vitek GPI cards. Antimicrobial susceptibility testing was evaluated by use of broth microdilution and Etest (AB BIODISK, Solna, Sweden methods. The isolate was identified as E. faecalis and was considered resistant to both vancomycin (MIC, > 256 mug/mL and teicoplanin (MIC, 256 mug/mL. The isolate also showed high level resistance to gentamicin and streptomycin (MICs, > 1024 mug/mL, but was considered susceptible to ampicillin (MIC, 4 mug/mL. Although the frequency of enterococcal infections is very low in most Latin America countries, the finding of glycopeptide (VanA resistance in E. faecalis increases concern about apreading antimicrobial resistance in this region.

  2. Arheozooloogilised leiud inimese elutegevuse uurimise allikana : Vana-Pärnu jäätmeauk 17. sajandist / Ingrid Õunapuu, Liina Maldre

    Index Scriptorium Estoniae

    Õunapuu, Ingrid

    2010-01-01

    Vana-Pärnu muinsuskaitsealal avastatud 17. sajandi jäätmeaugus sisaldunud arheozooloogilisest materjalist. Leitud luude määrangu ja analüüsi põhjal saab teha järeldusi selle ajastu inimtegevuse ja koduloomade kohta

  3. Mis juhtus Puhja kiriklas? : ühe vana tõlke algust otsides / Annika Kilgi, Inge Käsi, Aivar Põldvee ... [jt.

    Index Scriptorium Estoniae

    2014-01-01

    Otsitakse vastust küsimusele, kumb keel oli isa-poja Andreas ja Adrian Virginiuse tõlgitud Vana Testamendi sihtkeel, kas lõunaeesti või põhjaeesti keel ning kas Puhjas toimus midagi tõlketalgute taolist. Artikkel fikseerib uurimise hetkeseisu

  4. Moderate-Level Resistance to Glycopeptide LY333328 Mediated by Genes of the vanA and vanB Clusters in Enterococci

    Science.gov (United States)

    Arthur, Michel; Depardieu, Florence; Reynolds, Peter; Courvalin, Patrice

    1999-01-01

    Three of five natural plasmids carrying a wild-type vanA gene cluster did not confer LY333328 glycopeptide resistance on Enterococcus faecalis JH2-2 (MIC = 2 μg/ml). The two remaining plasmids conferred resistance to the drug (MIC, 8 μg/ml). The vanB gene cluster did not confer resistance to LY333328, since this antibiotic was not an inducer. Mutations in the vanSB sensor gene that allowed induction by teicoplanin or constitutive expression of the vanB cluster led to LY333328 resistance (MIC, 8 to 16 μg/ml). Overproduction of the VanH, VanA, and VanX proteins for d-alanyl-d-lactate (d-Ala-d-Lac) synthesis and d-Ala-d-Ala hydrolysis was sufficient for resistance to LY333328 (MIC, 16 μg/ml). Mutations in the host d-Ala:d-Ala ligase contributed to LY333328 resistance in certain VanA- and VanB-type strains, but the MICs of the antibiotic did not exceed 16 μg/ml. Addition of d-2-hydroxybutyrate in the culture medium of mutants that did not produce the VanH d-lactate dehydrogenase led to incorporation of this d-2-hydroxy acid at the C-terminal ends of the peptidoglycan precursors and to LY333328 resistance (MIC, 64 μg/ml). The vanZ gene of the vanA cluster conferred resistance to LY333328 (MIC, 8 μg/ml) by an unknown mechanism. These data indicate that VanA- and VanB-type enterococci may acquire moderate-level resistance to LY333328 (MIC ≤ 16 μg/ml) in a single step by various mechanisms. PMID:10428906

  5. Prevalance of vanA Gene in Vancomycine Resistant Staphylococcus Aureus (VRSA) Isolated from Chicken and Turkey Meat in Tehran, Iran

    OpenAIRE

    Amini, M; Hosseini Doust, SR. (PhD); Mohabati Mobarez, A. (PhD)

    2015-01-01

    Background and Objective: Staphylococcus aureus have been isolated frequently from the infection of blood, skin and soft tissue, and is one of the causative agents of food-borne illnesses. The purpose of this study was to investigate the occurrence of vanA in raw meat samples in Tehran, Iran. Material and Methods: Totally, 119 samples of chicken and turkey raw meat were cultured for Staphylococcus aureus. The Resistance to vancomycin was determined and Van A Gene detected by PCR method. Resul...

  6. Biochemical and Biophysical Characterization of an Unexpected Bacteriolytic Activity of VanX, a Member of the Vancomycin-resistance vanA Gene Cluster

    Science.gov (United States)

    Sohya, Shihori; Kamioka, Tetsuya; Fujita, Chisako; Maki, Tei; Ohta, Yoshihiro; Kuroda, Yutaka

    2014-01-01

    VanX is a d-alanyl-d-alanine (d-Ala–d-Ala) dipeptidase encoded in the vancomycin-resistance vanA gene cluster. Here we report that strong bacteriolysis occurred when isolated VanX was expressed in Escherichia coli at temperatures lower than 30 °C, which was unexpected because the vanA operon confers vancomycin resistance by protecting the cell wall. Therefore, we monitored cell lysis by measuring sample turbidity with absorbance at 590 nm and VanX expression using SDS-PAGE. No cell lysis was observed when VanX was expressed, even in large quantities, in the cell inclusion bodies at 37 °C, suggesting that a natively folded VanX is required for lysis. In addition, VanX mutants with suppressed dipeptidase activity did not lyse E. coli cells, confirming that bacteriolysis originated from the dipeptidase activity of VanX. We also observed shape changes in E. coli cells undergoing VanX-mediated lysis with optical microscopy and classified these changes into three classes: bursting, deformation, and leaking fluid. Optical microscopic image analysis fully corroborated our interpretation of the turbidity changes in the samples. From a practical perspective, the finding that VanX expressed in isolation induces cell lysis suggests that inhibitors of VanA and VanH that act downstream from VanX could provide a new class of therapeutic chemicals against bacteria expressing the vancomycin-resistance gene cluster. PMID:25294880

  7. Kuidas meie kasutatud arvutid Aafrikasse jõuavad / Aarne Ruben

    Index Scriptorium Estoniae

    Ruben, Aarne, 1971-

    2008-01-01

    Ettevõttest 3 Step IT, mis Soomes, Eestis, Suurbritannias, Rootsis, Taanis, Lätis, Norras ja Leedus klientidele arvutiparki rendib, paigaldab ja hoolitseb seadmete elutsükli eest. Reportaaž 3 Step IT Grupi ladustamisüksusest Vantaas. Vt. samas: Käive kasvab hüppeliselt

  8. Emergence of vanA vancomycin-resistant Enterococcus faecium in a hospital in Porto Alegre, South Brazil.

    Science.gov (United States)

    Resende, Mariah; Caierão, Juliana; Prates, Juliana Gil; Narvaez, Gabriel Azambuja; Dias, Cícero Armídio Gomes; d'Azevedo, Pedro Alves

    2014-02-13

    In Porto Alegre (South Brazil), since the first VRE isolation in 2000 until the middle of the last decade, the epidemiology of enterococcal infections presented the peculiarity that, as opposed to other regions of the country, almost all VRE were E. faecalis. The aim of this study was to investigate the microbiological and epidemiological characteristics of a VRE outbreak that occurred between August 2010 and September 2011 in Porto Alegre, South Brazil. Twenty-nine isolates from inpatients of Mãe de Deus Hospital that were identified and characterized for their susceptibility profile, vancomycin genotype, presence of esp gene, biofilm production, and clonal relationship were collected.  Patients' records were reviewed for clinical information. All isolates were identified as vancomycin/ampicillin resistant E. faecium carrying the vanA gene. Almost all were susceptible to gentamicin and streptomycin. Most patients died and were associated with a hemodialysis unit stay. All but the first isolate were clustered in a main clone. An important change in vancomycin-resistant enterococci was observed. Studies like this are necessary to monitor the dissemination of VRE, especially of some individual clones.

  9. Vancomycin-dependent Enterococcus faecium vanA: characterization of the first case isolated in a university hospital in Brazil

    Directory of Open Access Journals (Sweden)

    G. Kerbauy

    2011-03-01

    Full Text Available In this study, we report the characterization of a strain of Enterococcus faecium vanA, which grows only in the presence of vancomycin (VDEfm-UEL. The bacterium was isolated from the feces of a female patient who had undergone surgical treatment of Reinke’s edema and was receiving intravenous vancomycin therapy for infection with methicillin/oxacillin-resistant Staphylococcus aureus, a postoperative complication. Antimicrobial dependence was further confirmed by the vancomycin E-test. VDEfm-UEL was also shown to be resistant to ampicillin, ciprofloxacin, chloramphenicol, erythromycin, levofloxacin, penicillin, rifampicin, and teicoplanin. The putative virulence genes efaA, gelE and esp were detected by PCR. The ddl gene from VDEfm-UEL was cloned and sequenced. Vancomycin dependence seems to be associated with the insertion of a nucleotide in that sequence, which results in a frame-shift mutation, introducing a premature stop codon. This is the first report of vancomycin-dependent E. faecium isolation in a university hospital in Brazil.

  10. Genomic analysis of 495 vancomycin-resistant Enterococcus faecium reveals broad dissemination of a vanA plasmid in more than 19 clones from Copenhagen, Denmark

    DEFF Research Database (Denmark)

    Pinholt, Mette; Gumpert, Heidi; Bayliss, Sion

    2017-01-01

    OBJECTIVES: From 2012 to 2014, there has been a huge increase in vancomycin-resistant (vanA) Enterococcus faecium (VREfm) in Copenhagen, Denmark, with 602 patients infected or colonized with VREfm in 2014 compared with just 22 in 2012. The objective of this study was to describe the genetic......A plasmid was highly covered by reads from isolates containing the type 4 transposon. CONCLUSIONS: This study suggests that it was the dissemination of the type 4 Tn1546-like transposon and plasmid via horizontal transfer to multiple populations of E. faecium, followed by clonal spread of new VREfm clones...

  11. Multilevel population genetic analysis of vanA and vanB Enterococcus faecium causing nosocomial outbreaks in 27 countries (1986-2012)

    DEFF Research Database (Denmark)

    Freitas, Ana R; Tedim, Ana P; Francia, Maria V

    2016-01-01

    -2012). In total, 53 VanA and 18 VanB isolates (27 countries, 5 continents) were analysed and 82 vancomycin-susceptible E. faecium (VSEfm) were included for comparison. Clonal relatedness was established by PFGE and MLST (goeBURST/Bayesian Analysis of Population Structure, BAPS). Characterization of van...... transposons (PCR mapping, RFLP, sequencing), plasmids (transfer, ClaI-RFLP, PCR typing of relaxases, replication-initiation proteins and toxin-antitoxin systems, hybridization, sequencing), bacteriocins and virulence determinants (PCR, hybridization, sequencing) was performed. VREfm were mainly associated...

  12. Analysis of VanA vancomycin-resistant Enterococcus faecium isolates from Saudi Arabian hospitals reveals the presence of clonal cluster 17 and two new Tn 1546 lineage types

    NARCIS (Netherlands)

    M.A. Khan (Mushtaq); M. van der Wal (Martin); D.J. Farrell (David); L. Cossins (Luke); A.F. van Belkum (Alex); A. Alaidan (Alwaleed); J.P. Hays (John)

    2008-01-01

    textabstractObjectives; The aim of this study was to characterize 34 vancomycin-resistant VanA Enterococcus faecium isolates obtained from two hospitals in Saudi Arabia and to assess Tn 1546 variation within these isolates. Methods: PFGE and multilocus sequence typing (MLST) genotypes, antibiotic

  13. Metal. Uut ja vana

    Index Scriptorium Estoniae

    2001-01-01

    22. septembril esinesid Von Krahli Teatris neli eesti kolemuusikabändi. Heliplaatidest Forgotten Sunrise "a.Nimal f.Lesh - looma liha", Scavenger "Goattrend", Bestia "Demo", Celestial Crown "Invasion of Suicidal Angels"

  14. Uus pianistide põlvkond : seitsmes Eesti pianistide konkurss / Arbo Valdma, Lauri Väinmaa, Toomas Vana, Age Juurikas...[jt.] ; küsitlenud Nele-Eva Steinfeld

    Index Scriptorium Estoniae

    2008-01-01

    Intervjuu noorte pianistide konkursi žürii liikmete Arbo Valdma, Lauri Väinmaa, Toomas Vana, Age Juurikase ja Leelo Kõlariga Eesti Muusika- ja Teatriakadeemia ning Eesti Klaveriõpetajate Ühingu korraldatud VII Eesti pianistide konkursist. Konkursi võitjaks tuli Eesti Muusika- ja Teatriakadeemia üliõpilane Maksim Štšura. Auhinnalistele kohtadele tulid veel Ruslan Strogij, Margus Riimaa, Andre Hinn, Jaan Ots, Johan Randvere ja Aleksandra Kamenskaja

  15. Multilevel population genetic analysis of vanA and vanB Enterococcus faecium causing nosocomial outbreaks in 27 countries (1986-2012).

    Science.gov (United States)

    Freitas, Ana R; Tedim, Ana P; Francia, Maria V; Jensen, Lars B; Novais, Carla; Peixe, Luísa; Sánchez-Valenzuela, Antonio; Sundsfjord, Arnfinn; Hegstad, Kristin; Werner, Guido; Sadowy, Ewa; Hammerum, Anette M; Garcia-Migura, Lourdes; Willems, Rob J; Baquero, Fernando; Coque, Teresa M

    2016-12-01

    Vancomycin-resistant Enterococcus faecium (VREfm) have been increasingly reported since the 1980s. Despite the high number of published studies about VRE epidemiology, the dynamics and evolvability of these microorganisms are still not fully understood. A multilevel population genetic analysis of VREfm outbreak strains since 1986, representing the first comprehensive characterization of plasmid content in E. faecium, was performed to provide a detailed view of potential transmissible units. From a comprehensive MeSH search, we identified VREfm strains causing hospital outbreaks (1986-2012). In total, 53 VanA and 18 VanB isolates (27 countries, 5 continents) were analysed and 82 vancomycin-susceptible E. faecium (VSEfm) were included for comparison. Clonal relatedness was established by PFGE and MLST (goeBURST/Bayesian Analysis of Population Structure, BAPS). Characterization of van transposons (PCR mapping, RFLP, sequencing), plasmids (transfer, ClaI-RFLP, PCR typing of relaxases, replication-initiation proteins and toxin-antitoxin systems, hybridization, sequencing), bacteriocins and virulence determinants (PCR, hybridization, sequencing) was performed. VREfm were mainly associated with major human lineages ST17, ST18 and ST78. VREfm and VSEfm harboured plasmids of different families [RCR, small theta plasmids, RepA_N (pRUM/pLG1) and Inc18] able to yield mosaic elements. Tn1546-vanA was mainly located on pRUM/Axe-Txe (USA) and Inc18-pIP186 (Europe) plasmids. The VanB2 type (Tn5382/Tn1549) was predominant among VanB strains (chromosome and plasmids). Both strains and plasmids contributed to the spread and persistence of vancomycin resistance among E. faecium. Horizontal gene transfer events among genetic elements from different clonal lineages (same or different species) result in chimeras with different stability and host range, complicating the surveillance of epidemic plasmids. © The Author 2016. Published by Oxford University Press on behalf of the British

  16. Diversity of Tn1546 in vanA-positive Enterococcus faecium clinical isolates with VanA, VanB, and VanD phenotypes and susceptibility to vancomycin.

    Science.gov (United States)

    Cha, J O; Yoo, J I; Kim, H K; Kim, H S; Yoo, J S; Lee, Y S; Jung, Y H

    2013-10-01

    To investigate diversity in the vanA cluster in Enterococcus faecium isolates from nontertiary hospitals. We identified 43 vanA-positive Ent. faecium isolates, including two vancomycin-susceptible isolates, from hospitals between 2003 and 2006. Of these isolates, >85% were resistant to ampicillin, erythromycin and ciprofloxacin. The vanA cluster was classified into six types using overlapping PCR, but the prototype transposon Tn1546 was not found. Most vanA-positive vancomycin-resistant Enterococcus (VRE) carried IS1216V and belonged to Type III (58·1%) or Type II (20·9%). vanY, vanZ and IS1216V were observed in the left and right ends of Type III with long-range PCR. IS1216V was also observed within vanS and vanX in the two vancomycin-susceptible isolates and in two vancomycin-resistant isolates. No VRE isolates with VanB and VanD phenotypes contained point mutations in vanS, unlike in previous reports. Sequence types (STs) of all isolates belonged to clonal complex 17, and ST78 was predominant. Insertion sequences, especially IS1216V, cause structural variation in the vanA cluster. We report the first observation of vanY and vanZ at the left end of Tn1546 in clinical isolates. This is the first report of the frequency of vancomycin resistance and diversity of Tn1546 in vanA-positive Ent. faecium isolates from nontertiary hospitals. © 2013 The Society for Applied Microbiology.

  17. Dispersion of the vancomycin resistance genes vanA and vanC of Enterococcus isolated from Nile tilapia on retail sale: A public health hazard

    Directory of Open Access Journals (Sweden)

    Kamelia Mahmoud Osman

    2016-08-01

    Full Text Available Although normally regarded harmless commensals, enterococci may cause a range of different infections in humans, including urinary tract infections, sepsis, and endocarditis. The acquisition of vancomycin resistance by enterococci (VRE has seriously affected the treatment and infection control of these organisms. VRE are frequently resistant to all antibiotics that are effective treatment for vancomycin-susceptible enterococci, which leaves clinicians treating VRE infections with limited therapeutic options. With VRE emerging as a global threat to public health, we aimed to isolate, identify enterococci species from tilapia and their resistance to van-mediated glycopeptide (vanA and vanC as well as the presence of enterococcal surface protein (esp using conventional and molecular methods. The cultural, biochemical (Vitek 2 system and PCR results revealed eight Enterococcus isolates from the 80 fish samples (10% to be further identified as E. faecalis (6/8, 75% and E gallinarum (2/8, 25%. Intraperitoneal injection of healthy Nile tilapia with the eight Enterococcus isolates caused significant morbidity (70% within 3 days and 100% mortality at 6 days post injection with general signs of septicemia. All of the eight Enterococcus isolates were found to be resistant to tetracycline. The 6/6 E. faecalis isolates were susceptible for penicillin, nitrofurantoin, gentamicin, and streptomycin. On the other hand 5/6 were susceptible for ampicillin, vancomycin, chloramphenicol and ciprofloxacin. The two isolates of E. gallinarum were sensitive to rifampicin and ciprofloxacin and resistant to vancomycin, chloramphenicol and erythromycin. Molecular characterization proved that they all presented the prototypic vanC element. On the whole, one of the two vancomycin resistance gene was present in 3/8 of the enterococci isolates, while the esp virulence gene was present in 1/8 of the enterococci isolates. The results in this study emphasise the potential role

  18. Vana Maailma tegevuskunstimaastikel / Al Paldrok

    Index Scriptorium Estoniae

    Paldrok, Al, 1969-

    2011-01-01

    Rahvusvahelise tegevuskunstifestivali "Diverse Universe" toimumise kohad ja ajad Euroopas 2011. aastal. Euroopa tegevuskunstist Pariisis toimunud tänavafestivali "Linnakeskkonna kaos" ("Urban Disorder"), Poolas Piotrków Trybunalski nimelises linnas toimunud festivali (kuraator Arti Grabowski, Non Grata esines aktsiooniga "Force majeure") ja Berliinis toimunud festivali "Nälg" ("Hunger") näitel

  19. Vana kinematograafia kaitseks / Peeter Riba

    Index Scriptorium Estoniae

    Riba, Peeter, 1967-

    1999-01-01

    Raoul Ruiz'i 1997.a. ilmunud raamatu "Filmikunsti poeetika" ("Poétique du cinéma") arvustus. Lisa : Artikkel Raoul Ruiz'i (1941-2011) kohta Ephraim Katzi entsüklopeediast "The Film Encyclopedia": Harper Perennial, 1998

  20. Vana õpetaja meenutab / Valter Voole

    Index Scriptorium Estoniae

    Voole, Valter

    2000-01-01

    Meenutusi koolielust pärast Teist maailmasõda. Autorid: Ilmar Oja, Ella Laaneorg, Laine Mägi, Liia Toom, Volli Mäeumbaed, Milvi Mett, Aune Ork, Aavo Lind, Rein Väljas, Toivo Pruul, Leo Tikerpuu, Lembit Sauer, Erna Kivi (Raavel), Salme Raave-Sõeruer, Ervin Norgan, Peeter Luiga, Maimo Hõbessaar, Heino Topp, Eha Remmelkoor (Türnpuu), Hermi Vain, Peeter Laredei. Meenutatakse ka koolinõunik Ernst Ennot ; Ülevaade Käinast ja president Pätsi külaskäigust. Algus: 1999, 20. nov. Järgneb 11.,18.,22.,25.,29. jaan., 1;5;8;10;15;19;26;29. veebr., 2;4;7;11;21;25. märts, 1;4;8;11;18;25;29. apr., 4;6;9;11;13;16;20;23;27;30. mai, 3;6;10;13;17;20;27. juuni, 1;4;8. juuli, 12. aug

  1. Arvutid asuvad elu jäljendama / Ayad Royyuru ; tekst: Arko Olesk

    Index Scriptorium Estoniae

    Royyury, Ayad

    2009-01-01

    Bioloogia on muutumas üha enam arvuti abil tehtavaks teaduseks. Rahvusvahelisest projektist Genographic, mis püüab DNA abil kaardistada, milliseid teid pidi inimkond Aafrikast väljudes maailma vallutas. Vestlus IBM arvutusliku bioloogia uurimiskeskuse juhiga

  2. Pop : Kogumikud. Vana kool. Funk / Siim Nestor

    Index Scriptorium Estoniae

    Nestor, Siim, 1974-

    2004-01-01

    Heliplaatidest: Erinevad "The Third Unheard: Connecticut Hip Hop 1979-1983". Erinevad "Funk Drops 3: Breaks, Nuqqets and Rarities", George Duke "The Essential George Duke", The Jacksons "The Very Best of The Jacksons"

  3. Cifrar vanas esperanzas para descifrar la realidad

    Directory of Open Access Journals (Sweden)

    Mariluz Vallejo Mejía

    2007-01-01

    Full Text Available Se aborda el debate que han dado los medios impresos al manejo acomodaticio de las cifras en el gobierno del presidente Uribe. La vulnerabilidad del sistema colombiano de estadística, sujeto a una política que exige guarismos favorables, se demuestra con voces autorizadas de la opinión y se analiza desde la perspectiva de la teoría de la argumentación.

  4. Mis juhtub vanas elektrijaamas? / Harda Roosna

    Index Scriptorium Estoniae

    Roosna, Harda, 1956-

    2009-01-01

    Hea Energia Festivalil 22.-26. juulil Hiiumaal Kärdla elektrijaamas mängitavast DRAKAdeemia lavastusest "Ei teki ega kao" (autorid Kerli Adov, Jaan Aps, Liis Lukk, Hiri Müüripeal, Siim Nurklik, Kaur Riismaa). Lavastaja Siret Paju

  5. Vana arm ei roosteta / Maarja Vaino

    Index Scriptorium Estoniae

    Vaino, Maarja

    2005-01-01

    A. H. Tammsaare romaani "Ma armastasin sakslast" ilmumisest möödub 70 aastat. Tammsaare muuseum korraldab selle tähistamiseks 7. novembril koos Tallinna Ülikooli eesti kirjanduse õppetooliga konverentsi. Vt. ka sõnum lk. 19

  6. Viljandi Vana kalmistu kuulsused / Kadi Sassi

    Index Scriptorium Estoniae

    Sassi, Kadi

    2005-01-01

    Kunstnik F. B. Dörbeck (1799-1835), kunstnik G. Mooste (1885-1957), kunstnik V. Ormisson (1892-1941), kunstnik P. Kondas (1900-1985), kirjanik Fr. Kuhlbars (1841-1924), kirjanik A. Rennit (1860-1936), lavastaja ja näitleja H. Gleser (1893-1932), tantsija I. Urbel (1900-1983), fotograaf J. Riet (1873-1952), skulptor A. L. Weizenberg (1837-1921), professor Fr. Dryer (1879-1934)

  7. Uljas uus vana maailm / Raivo Kelomees

    Index Scriptorium Estoniae

    Kelomees, Raivo, 1960-

    2004-01-01

    Meediakunstniku staatusest postsotsialistlikus ühiskonnas, sõltuvusest ja ebamäärasusest transformeeruvas kunstitehnoloogilises keskkonnas, kus vastuolud ja üksteisemõistmine on lähemal vaatlusel paiguti ületamatud, arvamusfoon on muutuv ning kaootiline, kohati kanoniseeruv ning kommunikatsioon tihti negatiivne

  8. Korter-ateljee vanas Londoni laos

    Index Scriptorium Estoniae

    1999-01-01

    Londonis Swanyardis asuva kahekorruselise laohoone ümberehitus. Eraldatav klaasfassaad on asetatud vanale telliskiviküljele ning mõjub ekraanina. Osa laest on asendatud toasuuruse valguskatusega. Arhitektid: A. Carus & P. Saint-John

  9. Vana-aasta filmiõhtu

    Index Scriptorium Estoniae

    2002-01-01

    Kanal2s oli sügisel telesari eesti tuntud arstidest, keda on filmitud aasta vältel. Helle Karise portreefilmide sarja "Contra mortem" näidatakse ka 30. detsembril Kinomajas filmiõhtul. Kohtuda saab ka arstide endiga

  10. Vana tuuletallaja”: August Kitzbergi mälestused eesti autobiograafia tüvitekstina. ”The Windhover in Youth and Age”: August Kitzberg and the Founding Texts of Estonian Autobiography

    Directory of Open Access Journals (Sweden)

    Tiina Ann Kirss

    2012-04-01

    Full Text Available In 1924, renowned Estonian author August Kitzberg (1855-1927, whose “village tales”, plays, and journalistic pieces had been favourite reading material among the people for some 50 years, published his autobiography, entitled Ühe vana “tuuletallaja” noorpõlve mälestused (”An Old Windhover’s Memories of Youth”. Ostensibly, as Kitzberg claims in his foreword, the text was never meant to be published, and constituted his attempt, in 1916, to write “something like a family chronicle”. Compositionally heteroclite, Kitzberg’s life story is a sampling of a wide range of narrative strategies and styles, always hovering on the borders of written and oral discourse and not pretending to achieve stringent coherence. This is by no means to deny the text its “artfulness”: its very rusticality and simplicity is an effective ruse. Skilfully manoeuvring a ”tailor’s needle,” the author threads the piecework of his own life story into the heftier canvas of “History” (recalling the subtitle of Märt Mitt’s life story from the 1870s. More importantly, Kitzberg’s text is authored by a consummate journalist who astutely and deliberately makes visible broader dynamics driving social and cultural history in Estonia in the second half of the 19th century. ”The windhover” as autobiographical persona thus complements the public figure of the author, who had made his mark in the world of Estonian letters through his journalism, literary criticism, and theatre, as well as his original prose. In this paper I argue that Kitzberg’s autobiography is a foundation text of a ”national” autobiographical canon, and explore the implications of that statement. First, Kitzberg’s “Windhover” offered a formal “sampler” for narrative techniques at the threshold of oral and written culture. Also, the text makes visible models for telling a life: behind Kitzberg’s text one senses his own reading culture, which includes

  11. Hitler, Stalin ja Vana Toomas koos / Helen Eelrand

    Index Scriptorium Estoniae

    Eelrand, Helen, 1971-

    2004-01-01

    Ajalooliste esemete, eelkõige kommunistliku ja natsionaalsotsialistliku sümboolika müügist. Kommentaarid Riigikogu liikmelt Helir-Valdor Seederilt, okupatsioonimuuseumi töötajalt Rasmus Rausbergilt ja siseministeeriumi pressinõunikult Katrin Videselt. Lisa: Lenin läheb idablokis kaubaks

  12. Michelini vana juht hukkus, uus toob reformid / Peggy Hollinger

    Index Scriptorium Estoniae

    Hollinger, Peggy

    2006-01-01

    Pärast Michelini perefirma juhi Edouard Michelini surma uueks juhiks saanud Michel Rollier tekitab võimalike muutuste tõttu firma töötajates ärevust. Lisad: Michelini uus juht: suuri muutusi ei tule. Tabel: Tulemused

  13. Vana Euroopa, noor maailm / David Bloom, Bo Malmberg

    Index Scriptorium Estoniae

    Bloom, David

    2007-01-01

    Ilmunud ka: Molodjozh Estonii 5. juuni lk. 11. Euroopa rahvastik on vananemas ja seetõttu on paljud riigid teinud poliitilisi otsuseid, mille eesmärk on tõsta iivet, et Euroopa suudaks säilitada kõrge elatustaseme järgnevaikski aastakümneiks

  14. Vana foto vajab päästmist / Kristel Kirss

    Index Scriptorium Estoniae

    Kirss, Kristel, 1967-

    2008-01-01

    Fotod on osa perekonnaloost ja neid tasub säilitada. Fotograaf Mati Saksladu, AS Maksifoto müügijuht Tanel Trump ja Agnes Mesak Eurofotost vanade fotode taastamise ja koopiate tegemise võimalustest. Vanade fotode taastamisega tegelevate firmade loetelu

  15. Kursi koolkond juba 15 aastat vana / Reet Varblane

    Index Scriptorium Estoniae

    Varblane, Reet, 1952-

    2003-01-01

    Kursi koolkonna - P. Allik, A. Gulk, I. Kruusamäe, M. Mäetamm, P. Pangsepp, P. Pajos, K. Suitso, I. Suumann, R. Tüür - uute tööde väljapanek Tallinna Kunstihoones (22. III-21. IV) ja retrospektiivnäitus Kunstihoone galeriis (22. III-28. IV). 14. IV esinevad noored luuletajad

  16. Kui vana on su keha? / Evelin Kivilo-Paas

    Index Scriptorium Estoniae

    Kivilo-Paas, Evelin, 1979-

    2012-01-01

    Keha vanuse testimisest Polar BodyAge'i programmi abil. Treener Kristi Roosimägi analüüsib osalejate, TV3 uudisteankur Marek Lindmaa, ilmateadustaja Liis Lusmäe ja reporter Feliks Unduski tulemusi

  17. Determination of vehicular pollution in the road tunnel of Vana ...

    African Journals Online (AJOL)

    The combustion of fuels (gasoline, LPG, diesel oil, and so on ...) in vehicle engines produces a lot of substances and gases. Depending on their concentration, their chemical stability and their adverse effects on environment and human being, the most relevant compounds in the exhaust gas are SO2, CO, NOx, H2S and O3.

  18. Vana mees tahab koju / Ester Šank

    Index Scriptorium Estoniae

    Šank, Ester, 1956-

    1998-01-01

    Riigikohus arutab 1932. a. lapsendatud (1941. a. lapsendamine tühistatud) Ants Tederi lapseõiguste taastamist Jürgensite perekonnas. Kui kohus ennistaks Ants Tederi lapsendamise perek. Jürgensite poolt, kaotaksid D. Visnapuu ja T. Sildmäe õiguse hoonele Tartu mnt. 39

  19. Vanas stiilis väitekiri ja kaitsmine / Urve Lippus

    Index Scriptorium Estoniae

    Lippus, Urve, 1950-2015

    2014-01-01

    Arvustus: Tyrväinen, Helena. Kohti Kalevala-sarjaa : identiteetti, eklektisyys ja Ranskan jälki Uuno Klamin musiikissa. Helsinki : Suomen musiikkitieteellinen seura : Suomen musiikkikirjastoyhdistys, 2013. (Acta musicologica Fennica ; 30)

  20. Vana palkonni uus võimalus / Gea Velthut-Sokka

    Index Scriptorium Estoniae

    Velthut-Sokka, Gea, 1977-

    1999-01-01

    Aastaid tühjalt seisnud palkmaja suvilaks ja aastaringselt kasutatavaks elamuks renoveerimise võimalustest. Lõuna-Eestis asuvat palkmaja vaatavad üle arhitekt Andres Kadarik ja Eviko Ehituse juhataja Rein Murumägi, nende kommentaarid.

  1. Uus Euroopa on surnud, elagu vana Euroopa / Ahto Lobjakas

    Index Scriptorium Estoniae

    Lobjakas, Ahto, 1970-

    2008-01-01

    Autor leiab, et postmodernsesse Euroopasse uskujatele oli NATO tippkohtumine Bukarestis pettumus, Prantsusmaa ja Saksamaa keeldusid andmast Ukrainale ja Gruusiale NATO-liikmesuse tegevuskava (MAP), sest nende hinnangul rikkunuks see Euroopa jõudude tasakaalu

  2. Sama vana rumalus / John Kay ; tõlk. Erik Aru

    Index Scriptorium Estoniae

    Kay, John

    2007-01-01

    Autor meenutab juhtumit Inglise legendaarse Lloyd'siga paarkümmend aastat tagasi, kui Lloydsi sündikaatide üksteiselt edasikindlustuse ostmine viis selle kindlustusturu peaaegu kokkuvarisemiseni. Ta leiab, et uue aastatuhande struktureeritud laenuturud on korranud Lloyd'si juhtumit

  3. Vana Rootsi-Mihkli kiriku uus hingamine / Egle Tamm

    Index Scriptorium Estoniae

    Tamm, Egle, 1969-

    2002-01-01

    Rootsi-Mihkli kiriku kujunemisest. 5. V 2002. a. restaureeritud kiriku taaspühitsemine. Altarist (Joachim Ambrust), altarimaalist "Püha õhtusöömaaeg", kantslist (Christian Ackermann), sisekujundusest (Tiiu Lõhmus), kirikutekstiilidest jm. tagasijõudnud pärandist

  4. Vana hea tuttav korruptsioon / Avo Üprus

    Index Scriptorium Estoniae

    Üprus, Avo, 1954-

    2004-01-01

    Ilmunud ka: Meie Maa, 25. aug. 2004, lk. 2; Nädaline, 26. aug. 2004, lk. 4; Järva Teataja, 26. aug. 2004, lk. 2; Koit, 26. aug. 2004, lk. 6; Põhjarannik, 26. aug. 2004, lk. 2; Severnoje Poberezhje, 26. aug. 2004, lk. 2; Harjumaa, 27. aug. 2004, lk. 2; Valgamaalane, 28. aug. 2004, lk. 2; Pärnu Postimees, 3. sept. 2004, lk. 15; Vali Uudised, 3. sept. 2004, lk. 2. Vastukaja Viktor Vassiljevi artiklitele 19. juuli ja 17. aug. Postimehes

  5. "Vana hea aeg" baltisaksa mälestusteraamatus / Ahti Saares

    Index Scriptorium Estoniae

    Saares, Ahti

    2000-01-01

    Baltisakslastest memuariste iseloomustab romantilis-patriootiline suhtumine kodumaasse ja sellest lähtuv kultuuritoojalik, kõrgemat missiooni täitev hoiak. Võrdlus Stefan Zweigi, Sven Danelli ja Kaljo Villako mälestusteraamatutega

  6. Karje Vana Maailma äärelt / Sven Vabar

    Index Scriptorium Estoniae

    Vabar, Sven

    2005-01-01

    IX Pimedate Ööde Filmifestivali filme - dokumentaalfilm Islandi muusikast "Karjuv meistriteos" ("Screaming Masterpiece") : režissöör Alexander Ergis : Island 2005, mängufilm "Otsides Alexander'it" ("Looking for Alexander") : režissöör Francis Leclerc : Kanada 2004

  7. Galicia peab valima vana või uue / Allan Espenberg

    Index Scriptorium Estoniae

    Espenberg, Allan

    2005-01-01

    19. juunil toimuvad valimised Hispaania autonoomses piirkonnas Galicias. Arvamusküsitluste tulemused näitavad, et konservatiivset Rahvaparteid ja tema liidrit Manuel Fraga Iribarnet toetab 45 protsenti piirkonna valijatest

  8. Visionäär : internet hukutab vana kapitalismi / Joichi Ito ; interv. Kristjan Otsmann

    Index Scriptorium Estoniae

    Ito, Joichi

    2007-01-01

    Maailma juhtivaid riskikapitaliste vastab küsimustele, mis puudutavad ebavajaliku info probleemi internetis, tema kujutlust tuleviku veebiteenustest, võrkude avamisega kaasnevaid muudatusi, jagamismajanduse tulumudeleid

  9. Prügilill, vana hõng ja minimalism / Ene Läkk

    Index Scriptorium Estoniae

    Läkk, Ene, 1957-

    2000-01-01

    Kahest äärmusest praeguses aiakujunduses: minimalismist ja vanahõngulisest, hästi organiseeritud segadusest. Erinevatest materjalidest terrassist, "woodland'ist", moesolevatest prügitaimedest. Trendinäiteks Saksa saatkonna haljasalast Tallinnas (arhitekt R. Herms, teostus Ü. ja U. Grishakov). Soovitusi maastikuarhitekt Ü. Grishakovilt

  10. Vana Lada Euroopa idaosa endiselt müüduim / Tõnu Tramm

    Index Scriptorium Estoniae

    Tramm, Tõnu, 1968-

    2005-01-01

    Ilmunud ka: Delovõje Vedomosti 9. märts lk. 19. Lada Riva ehk VAZ 2105 ja 2107 müügiedust Kesk- ja Ida-Euroopas. Diagramm ja tabel: Müüduimad automargid Kesk- ja Ida-Euroopas 2003. ja 2004. aastal

  11. Villem Reiman ja eesti vana kirjakeel / Villem Reiman und das ältere Schriftestnische

    Directory of Open Access Journals (Sweden)

    Valve-Liivi Kingisepp

    2012-01-01

    Full Text Available Im vorliegenden Beitrag wird auf einen Aspekt des vielfältigen Lebenswerkes der nationalen Koryphäe Villem Reiman (1861–1917 – die Publikation der alten estnischsprachigen Texte und deren Kommentare – eingegangen. Zugleich wird eine Übersicht über die Tätigkeit der Forschungsgruppe für die altestnische Schriftsprache des Instituts für estnische und allgemeine Sprachwissenschaft der Universität Tartu bei der weiteren Bearbeitung und Analyse der Texte, die von Reiman präsentiert wurden, gegeben. Es werden in erster Linie die deutschsprachigen Aufsätze aus der Feder von Villem Reiman betrachtet. Am bedeutendsten davon sind die ausführlichen Einleitungen zu den gedruckten Predigten von Georg Müller und den gedruckten Kirchenbüchern von Joachim Rossihnius, die als Verhandlungen der Gelehrten Estnischen Gesellschaft [Õpetatud Eesti Seltsi toimetised] in den Jahren 1891 und 1898 erschienen sind. Aus den Texten der oben genannten Autoren haben die Mitglieder der Forschungsgruppe für die altestnische Schriftsprache die Untersuchungen „Georg Mülleri jutluste sõnastik“ [Das Vokabular der Predigten von Georg Müller] (2000 und „Joachim Rossihniuse kirikumanuaalide leksika“ [Lexik der Kirchenmanuale von Joachim Rossihnius] (2002 zusammengestellt.Des Weiteren werden in der vorliegenden Abhandlung die ältesten Archivfunde zu den estnischsprachigen Texten, die auf Anregung Villem Reimans in Druck gegeben und kommentiert wurden, präsentiert. Diese Archivfunde wurden von den Forschern der alten Schriftsprache im Sammelband „Eesti keele vanimad tekstid ja sõnastik“ [Die ältesten Texte und das älteste Vokabular der estnischen Sprache] (1997 zusammengefasst.Dank Villem Reiman wurden die Texte aus der Entstehungszeit des estnischsprachigen gedruckten Wortes den Zeitgenossen zugänglich gemacht. Reimans Abhandlungen über die alte Schriftsprache sind für die heutigen Forscher sehr wertvolle Quellen bei der Darstellung der Geschichte der estnischen Schriftsprache.

  12. Tallinna Lennujaama vana terminali renoveerimine = Renovation of Tallinn Airport's old terminal / Epp Lankots

    Index Scriptorium Estoniae

    Lankots, Epp, 1976-

    2005-01-01

    Arhitektid: Andres Põime, Reet Põime, Tiiu Truus. Tallinna Lennujaama reisijateterminali projekteerisid 1939. a. arhitektid Roman Koolmar ja Artur Jürvetson, hoone valmis Paula Koido rekonstrueerimisprojekti järgi. Ill.: 2 värv. sisevaadet, välisvaade

  13. Pädaste mõis pakub nii uut kui vana! / Aale Kask

    Index Scriptorium Estoniae

    Kask, Aale

    2008-01-01

    Renoveeritud Pädaste mõisa peahoonest (1875). Omanikud Imre Sooäär ja Martin Breuer. Mõisaansambli restaureerimine toimus aastail 1996-2008. Restaureerimisarhitekt Katrin Etverk. Sisearhitektuur: Imre Sooäär, Kärt Kukkur. Restoranisaal kasvab üle talveaiaks. Kärt Kukkuri, Katrin Etverki ja Ülle Grishakovi kommentaarid. Värv. välisvaade, 14 sisevaadet

  14. Junāgadhi raidkiri: kommunaalpropaganda Vana-India moodi / Martti Kalda

    Index Scriptorium Estoniae

    Kalda, Martti, 1973-

    2016-01-01

    Rudradāmani raidkirjast. Raidkiri on rajatud Sudarśana veehoidla parandamise ja renoveerimise auks. Raidkirja ajaloolisest taustast. Raidkirja teksti tõlge ja kommentaarid. Tegemist on (peale müntide) ainsa säilinud tekstiga vürst Rudradāmani ajast

  15. 50 aastat uut ja vana (mööblit) / Piret Õunapuu

    Index Scriptorium Estoniae

    Õunapuu, Piret, 1955-

    2005-01-01

    Tartu Kõrgema Kunstikooli mööbliosakonna näitusest "Mööbel. Uksed. Pööning. Vanad tehnikad ja nende uus elu" Eesti Rahva Muuseumi näitusemajas. Eksponeeritakse austria disaineri Koloman Moseri tooli, Edgar Johan Kuusiku rahvusromantilisi toole, Nigul Espe lauda, Priit Verlini, Rain Pluutuse, Ahti Lille jt. töid. 6 ill

  16. Eesti ja läti vana kirjakeele seminar / Väino Klaus

    Index Scriptorium Estoniae

    Klaus, Väino, 1949-

    2003-01-01

    Seminar "Läti ja eesti kirjakeele ühised juured" 18. 10. 2002 Eesti Keele Instituudis, kus räägiti varase kirjakeele tekke sarnastest mõjutajatest, Piibli ja muu vaimuliku kirjanduse tõlkimisest ning latgale kirjakeelest

  17. Vana kaader järel-eesti ajal võimalusi proovimas / Maie Kalda

    Index Scriptorium Estoniae

    Kalda, Maie, 1929-2013

    2001-01-01

    Artikkel põhineb konverentsil "Kohandumise märgid" (20. apr. 2001) peetud ettekandel. Kirjanike elu- ja loominguloost a-tel 1944-1948, lähemalt Mait Metsanurgast, Hugo Raudsepast, Friedebert Tuglasest ja Oskar Lutsust

  18. Väga suur ja vana, aga ikka pooleli / Alexander Birnbaum

    Index Scriptorium Estoniae

    Birnbaum, Alexander

    2007-01-01

    Bologna San Petronio Basilica ehk Püha Petroniuse katedraalist. Kiriku fassaadist, interjöörist, seal asuvast päikesekellast. Lühidalt Strasbourgi, Kölni, Siena, Milano ja Barcelona Sagrada Familia katedraalide ehitamisest

  19. Aasta parimad interjöörid muutsid vana trendikaks / Piret Tali

    Index Scriptorium Estoniae

    Tali, Piret, 1972-

    2001-01-01

    Eesti Sisearhitektide Liidu aastapreemiad: eramu sisekujunduse preemia - Tüüne-Kristin Vaikla ja Urmas Vaikla, meelelahutusliku interjööri preemia - kohvik "Pegasus" (Gert Sarv, Margus Tammik, Tarvo H. Varres osaühisusest Front Arhitektid), ajaloolise interjööri preemia - Tallinna Reaalkooli renoveerimisprojekt Tiina Lõhmuse juhtimisel, unikaaleseme preemia - Leila Pärtelpoja kujundatud riigikogu esimehe puhkeruumi mööbel Toompeal. Nimetatud žürii koosseis

  20. Vana platelsitsija juhtum: kapo töö kogemustepagas / Arvi Tap-Tapver

    Index Scriptorium Estoniae

    Tap-Tapver, Arvi

    2009-01-01

    Eesti Kaitsepolitsei uuris 1990-ndatel tollase Koonderakonna juhi ja Riigikogu riigikaitsekomisjoni esimehe Peeter Lorentsi seotust Soomest relvade salakaubana sissetoomisega mässulisele Läänemaa Vabatahtlike Jäägrikompaniile 1993. a., vahetult enne Pullapää kriisi

  1. Teatripinge vanas diiselelektrijaamas / Siret Paju, Liis Lukk, Veiko Tubin ; intervjueerinud Harda Roosna

    Index Scriptorium Estoniae

    Paju, Siret, 1982-

    2009-01-01

    Hea Energia Festivalil Hiiumaal Kärdla elektrijaamas mängitavast DRAKAdeemia lavastusest "Ei teki ega kao" (autorid Kerli Adov, Jaan Aps, Liis Lukk, Hiri Müüripeal, Siim Nurklik, Kaur Riismaa). Lavastaja Siret Paju

  2. Uus kui hästi unustatud vana : Raamaturida / Arno Oja

    Index Scriptorium Estoniae

    Oja, Arno, 1950-

    1995-01-01

    Waugh, Evelyn. Must murjan; Mirbeau, Octave. Toaneitsi päevik; Ödmann, Malin. Mõisa saladus; Dragt, Tonke. Kiri kuningale; Duncker, Dora. Louis XIV armuidüll; Lessing, Doris. Viies laps; Bullock, Michael. Kaev ja teisi jutte; Kaksteist Eesti Kirja

  3. Tähistaeval põhinevatest uskumustest III Vana-Hiina

    OpenAIRE

    Enn Kasak

    2002-01-01

    The article concerns Chinese astral myths, calendar and astrology. Chinese creation myths concern also the creation of the Sun, Moon and stars. The heavenly bodies are constantly endangered by the sky dragon that now and then catches them, probably a reference to eclipses. Cosmology existed in Ancient China in the same sense as in Ancient Greede, though many ancient books were burned on the emperor's orders in 213 BC. Teaching of the endless sky emerged in the Han era, while in 1 century AD t...

  4. "Baltoscandal" - jätkem vanas urgitsemine ja suunakem energia uue loomisele! / Evelin Lagle

    Index Scriptorium Estoniae

    Lagle, Evelin

    2008-01-01

    10. Baltoscandalil nähtust. Lavastustest: "50 Lovely Ways to Die", "Why Don't You Dance?", "I Apologize", "Anira", "Kool", "Võluflööt. Remix ja selgitus", "While We Were Holding It Together", "HarS"

  5. Rotermanni vana jahuladu ja uus jahuladu = Rotermann old and new flour storage / Hanno Grossschmidt, Tomomi Hayashi

    Index Scriptorium Estoniae

    Grossschmidt, Hanno, 1973-

    2009-01-01

    Tallinna endise Rotermanni tööstuskvartali arhitektuuri kaasajastamine. Arhitektid Yoko Azukawa, Hanno Grossschmidt ja Tomoni Hayashi, projekteerija HG Arthitektuur, konstruktor Neoprojekt. Projekt ja ehitus 2006-2008

  6. Gidon Kremer ja Tatjana Grindenko : vana arm ei roosteta! / Tatjana Grindenko ; interv. Aleksandra Moorast

    Index Scriptorium Estoniae

    Grindenko, Tatjana

    2007-01-01

    Viiuldajatest Gidon Kremerist ja Tatjana Grindenkost. Kontserdist Tallinna Polütehnilise Instituudi aulas 30 aasta eest. Kontserdist "Jubilate: Gidon Kremer 60 ja Kremerata Baltica 10" 11. veebr. Estonia kontserdisaalis

  7. Vana vimm lõi Maltal välja / Katrin Pauts

    Index Scriptorium Estoniae

    Pauts, Katrin, 1977-

    2003-01-01

    President Arnold Rüütli Malta riigivisiidi ajal ütles presidendi protokolliülem Andrei Birov välisministeeriumi protokolliosakonna juhatajale Tiina Nirgile "Fuck you!". Riigivisiit Malta Vabariiki 1.-3.10.2003

  8. Vana ja tuttav, aga liiga turvaline / Alar Niineväli

    Index Scriptorium Estoniae

    Niineväli, Alar

    2008-01-01

    Steven Spielbergi neljas Indiana Jones'i film Harrison Fordiga nimiosas "Indiana Jones ja kristallpealuu kuningriik" ("Indiana Jones and the Kingdom of the Crystal Skull") : Ameerika Ühendriigid 2008

  9. Uue ja vana vastaspinge Erich Jacoby arhitektuuris / Carl-Dag Lige

    Index Scriptorium Estoniae

    Lige, Carl-Dag

    2011-01-01

    Näitus "Arhitekt Erich Jacoby 125" Eesti Arhitektuurimuuseumis 15.12.2010-13.02.2011, kuraator Mait Väljas, kujundaja Marge Pervik-Kaal, fotograaf Martin Siplane. Erich Jacobyst, tema tegevusest ja loomingust

  10. Kunstiakadeemia uus õppehoone kõrgub õhuliselt vana maja kohale / Ants Juske, Mari Kodres

    Index Scriptorium Estoniae

    Juske, Ants, 1956-2016

    2008-01-01

    Eesti Kunstiakadeemia uue hoone arhitektuurivõistlusest ja võidutööst "Art Plaza"(Taani arhitektuuribüroo Effekt). Võistlustöödest "TLN247" (II koht), "Arts Factory" (III koht), "Public Section" (ergutuspreemia) ja "Sashimi" (ergutuspreemia)

  11. USA-s tõsteti terroriohtu kolm aastat vana info peale

    Index Scriptorium Estoniae

    2004-01-01

    Al-Qaida arvutieksperdilt Muhammad Naeem Noor Khaanilt, kes tabati Pakistanis, saadi kätte mitmeid dokumente, luureinfot ja skeeme. Enamik infot oli kogutud enne 11. septembril 2001. aastal toimunud terrorirünnakuid

  12. ML Arvutite aktsiakapital suurenes / Anne Oja

    Index Scriptorium Estoniae

    Oja, Anne, 1970-

    2006-01-01

    ML Arvutid omanik Aivar Paalberg tõstis aktsiakapitali seniselt 10 miljonilt 24 miljonile eesmärgiga tugevdada oma positsioone Eesti turul ja kasvada kiiremas tempos kui kogu turg. Diagramm: Majandusnäitajad

  13. 500 tudengiga aines kontrollib eksamitöid õppejõu eest arvuti / Anu Mõistlik

    Index Scriptorium Estoniae

    Mõistlik, Anu

    2003-01-01

    Tartu Ülikooli selle semestri rahvarohkeima, 500 kuulajaga sotsiaalkultuurilise psühholoogia loengukursuse lõpus kirjutasid tudengid eksamitööks valikvastustega testi, mida hakkavad kontrollima arvutid

  14. Art Depoo : äri- ja eluhoone Jahu tn. 12 / Vana-Kalamaja tn. 34, Tallinn = Art Depoo : apartment building on Jahu St. 12 / Vana-Kalamaja St. 34, Tallinn / Epp Lankots

    Index Scriptorium Estoniae

    Lankots, Epp, 1976-

    2005-01-01

    "Norma" vanasse tootmishoonesse on rajatud kunstigalerii ja 51 korteriga elamu. Projekteerija: On Arhitektid OÜ. Autor Harry Klaar. Projekt 2004, valmis 2005. Ill.: I, II ja III korruse plaan, 5 välis- ja 4 sisevaadet

  15. Aastaraamatu Vana Tallinn köidetes I-XXV (XXIX) ilmunud tekstid=In den Bänden I-XXV (XXIX) des Jahrbuches Vana Tallinn publizierte Texten / Helve Russak

    Index Scriptorium Estoniae

    Russak, Helve

    2014-01-01

    Artiklid kokkuvõtetega=Beiträge mit den Zusammenfassungen; Ülevaated, retsensioonid, annotatsioonid, arvamused=Übersichten, Rezensionen, Annotationen, Ansichten; Ex Archivo Civitatis Revaliensis; Mistsellid=Miszellen; Kroonika=Chronik; Järelhüüded=Nekrologen; Sündmused=Ereignisse; Mälestused ja ülevaated=Ereignisse und Übersichte; Saate- ja eessõnad=Zum Geleit und Vorwort; Vähetuntud pilte=Rare Bilder; Lisad=Anhänge

  16. Multiple hospital outbreaks of vanA Enterococcus faecium in Denmark, 2012-13, investigated by WGS, MLST and PFGE.

    Science.gov (United States)

    Pinholt, Mette; Larner-Svensson, Hanna; Littauer, Pia; Moser, Claus E; Pedersen, Michael; Lemming, Lars E; Ejlertsen, Tove; Søndergaard, Turid S; Holzknecht, Barbara J; Justesen, Ulrik S; Dzajic, Esad; Olsen, Stefan S; Nielsen, Jesper B; Worning, Peder; Hammerum, Anette M; Westh, Henrik; Jakobsen, Lotte

    2015-09-01

    In Denmark, the incidence of vancomycin-resistant Enterococcus faecium (VREfm) has increased since 2012. The aim of this study was to investigate the epidemiology and clonal relatedness of VREfm isolates in Danish hospitals in 2012-13 using WGS. The second aim was to evaluate if WGS-based typing could replace PFGE for typing of VREfm. A population-based study was conducted including all VREfm isolates submitted for national surveillance from January 2012 to April 2013. All isolates were investigated by WGS, MLST and PFGE. One-hundred and thirty-two isolates were included. The majority of the isolates were from clinical samples (77%). Gastroenterology/abdominal surgery (29%) and ICUs (29%) were the predominant departments with VREfm. Genomics revealed a polyclonal structure of the VREfm outbreak. Seven subgroups of 3-44 genetically closely related isolates (separated by WGS. Direct or indirect transmission of VREfm between patients and intra- and inter-regional spreading clones was observed. We identified 10 STs. PFGE identified four major clusters (13-43 isolates) and seven minor clusters (two to three isolates). The results from the typing methods were highly concordant. However, WGS-based typing had the highest discriminatory power. This study emphasizes the importance of infection control measures to limit transmission of VREfm between patients. However, the diversity of the VREfm isolates points to the fact that other important factors may also affect the VREfm increase in Denmark. Finally, WGS is suitable for typing of VREfm and has replaced PFGE for typing of VREfm in Denmark. © The Author 2015. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

  17. Vana hea arhitektuur : Villa-V Nõmmel = Good Old Architecture : Villa-V in Nõmme / Siiri Vallner

    Index Scriptorium Estoniae

    Vallner, Siiri, 1972-

    2001-01-01

    Projekteerija: arhitektibüroo Kolm Pluss Üks. Arhitektid Markus Kaasik, Andres Ojari, Ilmar Valdur. Projekt 1998, valmis 2001. 11 ill.: pindade diagramm, korruste plaanid, lõige, sise- ja välisvaated

  18. Multiple hospital outbreaks of vanA Enterococcus faecium in Denmark, 2012-13, investigated by WGS, MLST and PFGE

    DEFF Research Database (Denmark)

    Pinholt, Mette; Larner-Svensson, Hanna; Littauer, Pia

    2015-01-01

    OBJECTIVES: In Denmark, the incidence of vancomycin-resistant Enterococcus faecium (VREfm) has increased since 2012. The aim of this study was to investigate the epidemiology and clonal relatedness of VREfm isolates in Danish hospitals in 2012-13 using WGS. The second aim was to evaluate if WGS...

  19. Peapiiskop pühitses Anija valla Pikva külla toodud vana kabeli / Külli Koppelmaa

    Index Scriptorium Estoniae

    Koppelmaa, Külli, 1970-

    2005-01-01

    1891. a. arhitekt Erwin von Bernardti projekti järgi valminud puitkabel Tallinnast toodi Pikva mõisa ja sai nimeks Pickwa Miikaeli kabel. Taastamisprojekti autor Hillar Kannelmäe OÜ Kuukaarest, altarimaali autor Lauri Sillak, tekstiilide autor Marit Kuivonen. 1851. aastal Hans Holmi ehitatud kapporel on pärit Kolga mõisast. Anija vallavalitsuse andmeil puudub kabeli ehitusluba

  20. Nad ikka said selle Kärdla vana elektrijaama tööle / Bianca Mikovitš

    Index Scriptorium Estoniae

    Mikovitš, Bianca

    2009-01-01

    Siret Paju lavastusest "Ei teki ega kao" Kärdla elektrijaamas. Näidendi autorid Kerli Adov, Jaan Aps, Liis Lukk, Hiri Müüripeal, Siim Nurklik ja Kaur Riismaa. Projekti käivitas MTÜ Arhipelaag eesotsas Ere Naadiga

  1. Sky Plus maksuvõlglaste esikümnes vana võlaga / Ralf-Martin Soe

    Index Scriptorium Estoniae

    Soe, Ralf-Martin

    2008-01-01

    Sky Plusi poolest tuntud raadiofirma First Media maksuvõlg oli 2007. aasta lõpuks 44,5 miljonit krooni. Vt. samas: Nõue aastatetaguse agendilepingu eest. Diagramm: Majandusnäitajad; Maksuvõlglaste edetabel

  2. "Tartu Athena" tarib viimase hetke majja kunsti / Aigi Viira

    Index Scriptorium Estoniae

    Viira, Aigi

    1999-01-01

    Kunstiprojekt ("Vana Athena" muudeti "Vanaks Anatoomikumiks") Tartu Vanas Anatoomikumis. Kuraator Paul Rodgers. Osalejad Inglismaalt, Soomest, Saksamaalt, Leedust, Rumeeniast, Venemaalt, Šveitsist, Eestist

  3. Arvutitootjad ootavad innuga sügist / Alar Pardla

    Index Scriptorium Estoniae

    Pardla, Alar, 1970-

    2004-01-01

    Ilmunud ka: Delovõje Vedomosti 1. sept. lk. 17. Arvutite tootmisest Eestis juulis. Diagramm: Viis suuremat võtavad endale 89,3% turust. Tabelid: ML Arvutid jätkab arvutite tootmises esikohal; Ordi on endiselt suurim sülearvutitootja

  4. Eesti koolid ei jaksa enam Microsoftilt tarkvara osta / Teele Tammeorg ; kommenteerinud Rain Laane

    Index Scriptorium Estoniae

    Tammeorg, Teele

    2013-01-01

    Eesti koolide arvutid on nii vananenud, et ei saa kasutada uuemaid operatsioonisüsteeme, Windowsi operatsioonisüsteem ja MS Office'i kasutamine aga kallineb vähemalt kümme korda. Arvamust avaldab ka Tallinna ülikooli haridustehnoloogia keskuse juht Mart Laanpere

  5. Mööbel vajab nišše ja ekspordiauke. Vallutamist ootab vana Euroopa / Ain Alvela ; kommenteerinud Ari Lustila, Heido Vitsur, Priit Tamm

    Index Scriptorium Estoniae

    Alvela, Ain, 1967-

    2011-01-01

    Olukorrast mööbliturul: edukamad on olnud korpusmööbliga tegelevad ettevõtted. Arvamust avaldavad ASi Suwem turundusjuht Mart Nilson ja OÜ Bellus Furnitur tootmisjuht Rolf Kristian Relander. Diagrammid

  6. Moodsad ajad vanas majas : Raekoja 1975. aasta sisekujundus = Modern Times at an Old Building : 1975 Interior Design of Tallinn Town Hall / Kai Lobjakas

    Index Scriptorium Estoniae

    Lobjakas, Kai, 1975-

    2004-01-01

    1973. a. Tallinna Raekojale uue sisekujunduslahenduse saamiseks korraldatud kutsutud konkursi võitis töö Annunciation, mille põhiautoriks oli Leila Pärtelpoeg, kaasautoriteks Aate-Heli Õun, Anu Raud, Ilme-Anu Neemre. Tööd jätkas Leila Pärtelpoeg koos Udo Umbergiga. Rippvaipade galerii autor Valve Ilveste

  7. Kirjanike majas lehvis vana biitniku vaim / Marko Mägi ; komment. Jürgen Rooste, Tõnu Õnnepalu, Contra, Lauri Kitsnik

    Index Scriptorium Estoniae

    Mägi, Marko

    2003-01-01

    Ginsberg, Allen. Ameerika : valik luuletusi 1947-1996 / koostanud ja toimetanud Hasso Krull ; inglise keelest tõlkinud Contra, Lauri Kitsnik, Hasso Krull, Andres Langemets, Margit Langemets, Jürgen Rooste, Tõnu Õnnepalu. Tallinn : Perioodika, 2003

  8. Et saada riigireformi 1. juuliks / Mart Raudsaar

    Index Scriptorium Estoniae

    Raudsaar, Mart, 1973-

    2016-01-01

    Võro Seltsi ettepanekust, et üheks võiks liituda kõik Vana Võromaa territooriumile jäävad 25 omavalitsust ning Vana Võromaa kunagised kaheksa kihelkonda moodustaksid uue omavalitsuse osavallad

  9. Mõiste saddiq/dikaios arengust : katse rakendada Lazar Gulkowitschi mõisteloolist meetodit algkristluse uurimisel / Urmas Nõmmik

    Index Scriptorium Estoniae

    Nõmmik, Urmas, 1975-

    2009-01-01

    Mõiste "õige inimene" kasutusest Vanas ja Uues Testamendis, apokrüüfses kirjanduses ning Qumrani kogudes. Septuaginta (ladina keeles 'seitsekümmend') on heebreakeelse Vana Testamendi vanim teadaolev täielik tõlge kreeka keelde

  10. Vladimir Etush nossil brilliantõv lombard / Oleg Peranov

    Index Scriptorium Estoniae

    Peranov, Oleg

    2004-01-01

    Muusikaline muinasjutt Hans Christian Anderseni ainetel "Vana, vana muinasjutt" ("Staraja, staraja skazka") : režissöör Nadezhda Kosheverova : Nõukogude Liit 1970. Tegijad meenutavad filmi ja selle tegemist

  11. Egiptuse kultused Kreeka-Rooma maailmas / Jaan Lahe

    Index Scriptorium Estoniae

    Lahe, Jaan, 1971-

    2004-01-01

    Vana-Egiptuse, Vana-Kreeka ja Vana-Rooma kultuurikontaktidest ning nende laienemisest peale Aleksander Suure vallutusi. Egiptuse lülitumisest hellenistlikku kultuurisüsteemi, Serapise, Isis, Osirise, Harpokratese kultustest Kreekas ja Roomas. Herodotose egiptuse kultuuri kirjeldustest "Historia" II raamatus.

  12. Puderkapsaatika ajaloomälestistega : uus kaitsenimekiri on vastu võetud, vana tühistamata. Segadus ajaloomälestistega. Milline on ajaloomälestiste staatus? / Jüri Kuuskemaa

    Index Scriptorium Estoniae

    Kuuskemaa, Jüri, 1942-

    1997-01-01

    Järg Mar/5;12 lk. 20. Kommentaar kultuurimälestiste loetelule, ilmunud Riigi Teataja Lisas . 119/120 1. nov. 1996 art. 628 kultuuri- ja haridusministri määruse (n. 19/2 19.VII 1995) põhjal 'Kultuurimälestiseks tunnistamine'. Ajaloomälestised. Kunstimälestised

  13. Arvutimaailm aastal 2009

    Index Scriptorium Estoniae

    2008-01-01

    Eesti info- ja kommunikatsioonitehnoloogia sektori tulevikku prognoosivad Webmedia Eesti tegevjuht Taavi Kotka, Mandatori nõukogu esimees Niilo Saard, Microsoft Eesti avaliku sektori suurkliendihaldur Rasmus Reimo, Telegrupi juhatuse esimees Ivo Remmelg, 3 Step IT Baltikumi juht Aivar Kraus, MicroLinki juhatuse esimees Enn Saar, Linxtelecom Estonia tegevjuht Erki Urva, Norby juhatuse esimees Oleg Shvaikovski, ML Arvutid juhatuse esimees Mait Rahi, Tele2 juhatuse esimees Toomas Tiivel, Elisa juhatuse liige Andrus Hiiepuu, IT Grupi nõukogu esimees Jaak Ennuste, HP Tallinna kontori ja konsultatsiooniteenuste juht Tõnis Mäe ja EMT arendus- ja tehnoloogiadirektor Tõnu Grünberg

  14. Dva soldata - nemetski i sovetski - risovali Narvu / Irina Tokareva

    Index Scriptorium Estoniae

    Tokareva, Irina

    2003-01-01

    Vana-Narva kujutisi: Carl Sarapi fotoalbum "Vana Narva" (1939), Anton Weiss-Wendti koostatud Vana-Narva fotoajalugu "Must-valge linn", saksa sõduri-kunstniku Senke Nisse joonistused 1944. aastast, vene sõduri Nikolai Tihhonovi joonistused varemetes Narvast. Kahe sõduri tööde koopiatest koostas muinsuskaitsja Jüri Tõnisson näituse Narva gümnaasiumis. Jüri Tõnissoni kommentaarid

  15. Data turbiini monoloog / Kai Lobjakas

    Index Scriptorium Estoniae

    Lobjakas, Kai, 1975-

    2007-01-01

    Kristiina Uslari võrgutaolisest klaasskulptuurist, mis valmistatud sulatatud klaasipurust ja mille algtõukeks on olnud Vana-Rooma topeltseinalised klaasanumad oma seniavastamata ülikeerulise tehnoloogiaga

  16. Protest calls for Ignalina reprieve / Kimberly Kweder

    Index Scriptorium Estoniae

    Kweder, Kimberly

    2007-01-01

    Vilniuses toimus protest vana Ignalina tuumaelektrijaama sulgemise vastu. Kardetakse sulgemise mõju majanduse stabiilsusele. Euroopa Komisjon kahtleb Ignalina tuumajaama reaktori sulgemise ajakavas muutuste tegemise vajalikkuses

  17. Tshelovek iz Ganzõ / Anna Litvinjuk

    Index Scriptorium Estoniae

    Litvinjuk, Anna

    2002-01-01

    Volgogradist pärit Boris Gavrilovi elust ja tema harrastusest -Tallinna vanalinna (ka teiste linnade) hoonete keraamiliste mini-koopiate valmistamisest, Hansalandi (Vana-Tallinn miniatuuris) projektist

  18. Leonardo da Vinci programmiga Euroopasse / Maie Üürike

    Index Scriptorium Estoniae

    Üürike, Maie

    2009-01-01

    Elukestva õppe allprogrammist, mis on suunatud kutsehariduse ja -koolituse edendamisele Euroopas. Vana-Vigala Tehnika- ja Teeninduskooli õpilased viibisid programmi raames erialasel praktikal Soomes

  19. Saalivalvurid varastavad ka Barcelonas

    Index Scriptorium Estoniae

    2000-01-01

    Hispaania politsei tabas Barcelona Arheoloogiamuuseumi saalivalvuri Manuel Gasca, kes varastas muuseumi hoidlaist umbes 35 miljoni Eesti krooni ulatuses vana-egiptuse, foiniikia, etruski ja vana-rooma arheoloogilisi leide (mündid, keraamika, skulptuur jt.) ning itaalia graafiku Giambattista Piranesi 150 gravüüri.

  20. Tartu toomhärrade päritolu = Social origins of Tartu canons / Tõnis Lukas

    Index Scriptorium Estoniae

    Lukas, Tõnis, 1962-

    1993-01-01

    Lisana tabelid: Vana-Liivimaalt pärit toomhärrade jaotumine ja osakaal. Lk. 23 ; Väljastpoolt Vana-Liivimaad pärinevad toomhärrad Tartu toomkapiitlis. Lk. 24 ; Tartu toomhärrade sotsiaalne päritolu. Lk. 25

  1. Aga missugused silmad tal olid? / Pille-Riin Purje

    Index Scriptorium Estoniae

    Purje, Pille-Riin, 1963-

    2010-01-01

    VAT Teatri lavastusest "... viimane lint / Saare esimene lint", kus põimuvad Tanel Saare esitus ja Jüri Järvet vana Krappina ("Krappi viimane lint") ekraanil. Tanel Saar lindistab S. Becketti "Krappi viimase lindi" salvestatud osa, mille kuulamine taaslavastatakse 30 aasta pärast, kui T. Saar ise on juba vana Krappi eas

  2. Tanel Saare omapärane duett Jüri Järvetiga / Tiiu Laks

    Index Scriptorium Estoniae

    Laks, Tiiu, 1984-

    2010-01-01

    VAT Teatri lavastusest "... viimane lint / Saare esimene lint", kus põimuvad Tanel Saare esitus ja Jüri Järvet vana Krappina ekraanil. Tanel Saar lindistab S. Becketti "Krappi viimase lindi" salvestatud osa, mille kuulamine taaslavastatakse 30 aasta pärast, kui T. Saar ise on juba vana Krappi eas

  3. Teatritükk, mille järgmist osa näeb 2040. aastal / Mari Hiiemäe

    Index Scriptorium Estoniae

    Hiiemäe, Mari

    2010-01-01

    VAT Teatri lavastusest "... viimane lint / Saare esimene lint", kus põimuvad Tanel Saare esitus ja Jüri Järvet vana Krappina ("Krappi viimane lint") ekraanil. Tanel Saar lindistab S. Becketti "Krappi viimase lindi" salvestatud osa, mille kuulamine taaslavastatakse 30 aasta pärast, kui T. Saar ise on juba vana Krappi eas

  4. Koomas ärimees ostis väärtusliku kinnistu? / Ülo Russak

    Index Scriptorium Estoniae

    Russak, Ülo

    2008-01-01

    Siseministeerium müüs enampakkumisel Kuusalu vallas Turbuneeme külas oleva vana piirivalvekordoni ärimees Lembit Kirsmele, kuigi vana kordon plaaniti merehariduskeskuseks ümber ehitada. Noortejuhi Linda Metsaorgi ja Harju maavanema Värner Lootsmanni kommentaare

  5. Talu tuulte teel / Merle Kares

    Index Scriptorium Estoniae

    Kares, Merle

    2005-01-01

    Taluümbruse korraldamisel on oluline tuuli vaigistada. Perekond Rummu Kuusalu vallas oleva aia rajamiseks koostas näidisprojekti Merle Kares. Krundil on kaks elamut, ühes elab vana- ja teises noorpere. Ill.: vana aia vaade, uus aiaplaan

  6. Kolm vaadet Tallinna vanalinnale / Villem Tomiste, Martin Aunin, Robert Treufeldt ; intervjueerinud Ingrid Ruudi

    Index Scriptorium Estoniae

    Tomiste, Villem, 1975-

    2009-01-01

    Tallinna vanalinna ja kesklinna piiril asuv kvartal 57B, mis piirneb Aia, Vana-Viru, Inseneri tänava ja Mere Puiesteega, ajendas vanalinna hoonestuspõhimõtete ja tulevikulahenduste üle arutama arhitekte Villem Tomistet, Martin Auninit ning arhitektuuriajaloolast, ICOMOSi Eesti komitee esimeest Robert Treufeldti. Pikemalt korterelamust Aia 4/Vana-Viru 13, Aia 6 ja 8 detailplaneeringu eskiisist

  7. Narva v miniatjure / Jevgeni Ashihmin

    Index Scriptorium Estoniae

    Ashihmin, Jevgeni

    2008-01-01

    2010. aastaks on kavas rajada Narvas mini-park "Vana-Narva miniatuuris". Puust ja kivist sõjaeelse Narva makett ehitatakse mastaabis 1:13. Projekti juhib Vadim Orlov. Narva raekojas avati Vana-Narva hoonete makettide (1:25) näitus. Valmistas Fjodor Shantsin

  8. 108aastase Liviko tooted koguvad tunnustust nii kodus kui välismaal / Tiit Reinart

    Index Scriptorium Estoniae

    Reinart, Tiit, 1964-

    2006-01-01

    Tänavu tunnustati AS-i Liviko tooteid Vana Tallinna Liköör, Üx Vodka ja Vana Tallinn Cream ühel mainekamal rahvusvahelisel konkursil International Wine & Spirit Competition (IWSC), ühtlasi tegeleb ettevõte jätkuvalt tootearenduse ja kvaliteetveinide importimisega. Vt. samas: Maksumärgita alkohol on illegaalne

  9. Võitlus tori tõuraamatute pärast jätkub / Aive Sarjas

    Index Scriptorium Estoniae

    Sarjas, Aive

    2012-01-01

    Eesti Hobusekasvatajate Seltsi (EHS) kaebusest kohtule, et seoses Veterinaar- ja toiduameti otusega tunnustda Vana-Tori Hobuse Ühingut tori tõugu hobuste vana-tori suuna tõuraamatu pidajana ei saa EHS täita aretus- ja säilitusprogrammiga võetud kohustusi

  10. Liivimaa maapäev Wolter von Plettenbergi ajal (1494-1535) / Pärtel Piirimäe

    Index Scriptorium Estoniae

    Piirimäe, Pärtel, 1972-

    2008-01-01

    Vana-Liivimaa poliitilise olukorra senistest käsitlustest. Radziwilli memorandumile rajatud maapäeva käsitluse kriitika. Vana-Liivimaa maapäevast kui huvitavast sünteesist ühelt poole territooriumide läbirääkimistest ning teiselt poolt klassikalisest seisuslikust kogunemisest. Maapäeva toimumisaegadest ja kokkukutsumistest. Lähemalt 1498. aasta maapäevast. Maapäeva otsustamisprintsiipidest ja esindajate mandaatidest. Maapäeva funktsioonidest ja rollist Vana-Liivimaa poliitilises süsteemis

  11. Through the Baltic States in a 2011 Jaguar XJ / Jamie Kitman

    Index Scriptorium Estoniae

    Kitman, Jamie

    2011-01-01

    Artikli autor külastab vana tuttavat, kellest nüüd on saanud Eesti president. Toomas Hendrik Ilves meenutab oma noorusaega ja elu kuni presidendiks saamiseni. President annab ülevaate elust Eestis ning riigi kohast maailmas

  12. Keele salaelu / Kristiina Ross

    Index Scriptorium Estoniae

    Ross, Kristiina

    2004-01-01

    Vana Testamendi eestikeelsest tõlkest, mille tegid ajavahemikul 1687-1690 isa ja poeg Andreas ja Adrian Virginius. Rmt.: Esimene Moosese raamat. Iiobi raamat / tõlkinud 1687-1690 Andreas ja Adrian Virginius. Tallinn, 2003

  13. Aserisse kerkib 250 miljonit krooni maksev tellisetehas / Gerli Romanovitsh

    Index Scriptorium Estoniae

    Romanovitš, Gerli, 1977-

    2005-01-01

    Ilmunud ka: Põhjarannik 19. mai 2005, lk. 1, Severnoje Poberezhje 19. mai 2005, lk. 1,3. AS Wienerburger kavatseb rajada Aserisse 250 miljonit krooni maksva tellisetehase, vanas vabrikus hakatakse tootma keraamilisi plokke

  14. Modus Vivendi ehk elulaad / Ivar Leimus

    Index Scriptorium Estoniae

    Leimus, Ivar, 1953-

    2003-01-01

    Rahvusvahelise konverentsi "Modus vivendi: linlaste igapäevaelu, mentaliteet, kultuur" ettekannetest : 14.-16. juuni 2002. Rets. rmt.: Vana Tallinn XII (XVII). Modus vivendi / vastutav toimetaja Raimo Pullat. Tallinn, 2002

  15. Terror USAs andis hoobi Eesti kõurikute koolile / Kaarel Kaas

    Index Scriptorium Estoniae

    Kaas, Kaarel, 1978-

    2002-01-01

    Ameerika rahvuskaardi egiidi all tegutsevalt programmilt "Rahvusvaheline oliivioks" oleks pidanud tulema ligi 10 mln kr. 5-kuuliste sõjaväelise sisekorraga kursuste jaoks Vana-Vigalas, kuid praegu on see projekt lahtine

  16. Translating ancient Greek aspect: Sappho's Fr. 1 Voigt / Janika Päll

    Index Scriptorium Estoniae

    Päll, Janika, 1965-

    2007-01-01

    Vana-kreeka keele aspektist, selle kasutamisest ja tõlkimisprobleemidest Sappho luuletuse tõlkeid analüüsides. Lisas toodud ka Sappho luuletus originaalkeeles, tõlked ladina, eesti, inglise, saksa, itaalia, prantsuse ja vene keelde

  17. Libahundi kujutelmade teoloogilis-demonoloogilised tõlgendused / Tiina Vähi

    Index Scriptorium Estoniae

    Vähi, Tiina

    2006-01-01

    Libahundikujutelmade lähtekohtadest. Libahundipildist vana- ja keskaja teoloogias ja demonoloogias. Lükantroopiast kui doktriinist varauusaegsetes teoloogilis-demonloogilistes traktaatides. XVII sajandi Liivi- ja Kuramaa vaimulike Herman von Samsoni ja Paul Einhorni lükantroopiakäsitlustest

  18. Von Krahl toob lavale poeetilise loomisloo / Merit Kask

    Index Scriptorium Estoniae

    Kask, Merit

    2007-01-01

    9. apr. Tallinna vana sadama tollilaos esietenduvast Peeter Jalaka lavastatud "Õnne valemist", muusika briti heliloojalt Gavin Bryarsilt, lavastus põhineb Stephen Hawkingi raamatul "Aja lühiajalugu"

  19. V kakoi zemle - nevedomo ... / Eteri Kekelidze

    Index Scriptorium Estoniae

    Kekelidze, Eteri, 1944-

    2007-01-01

    9. apr. Tallinna vana sadama tollilaos esietenduvast Peeter Jalaka lavastatud "Õnne valemist", muusika briti heliloojalt Gavin Bryarsilt, lavastus põhineb Stephen Hawkingi raamatul "Aja lühiajalugu"

  20. Kosmoloogia ja Gavin Bryarsi muusika ruum / Gavin Bryars ; interv. Immo Mihkelson

    Index Scriptorium Estoniae

    Bryars, Gavin

    2007-01-01

    9. apr. esietendub Tallinna vana sadama tollilaos Peeter Jalaka lavastatud "Õnne valem", mille muusika on briti heliloojalt Gavin Bryarsilt ning mis põhineb Stephen Hawkingi raamatul "Aja lühiajalugu"

  1. Prantsuse sotsialistid pole ikka Royaliga rahul / Hent Kalmo

    Index Scriptorium Estoniae

    Kalmo, Hent, 1979-

    2007-01-01

    Prantsusmaa sotsialistide presidendikandidaat Segolene Royal kutsus oma valimiskampaanias osalema partei nn. vana kaardiväe. Paljud sotsialistide juhid on Royali seisukohti pidanud liiga parempoolseiks, Royal on püüdnud oma valijaid veenda oma sotsialistlikus maailmavaates

  2. Carriage frequency, phenotypic, and genotypic characteristics of methicillin-resistant Staphylococcus aureus isolated from dental health-care personnel, patients, and environment

    National Research Council Canada - National Science Library

    Ahmed S Khairalla; Reham Wasfi; Hossam M Ashour

    2017-01-01

    .... Antibiotic susceptibility, biofilm formation, Staphylococcal protein A (spa) typing, SCCmec typing, and PCR-based assays were used to detect mecA, mecC, vanA, Panton-Valentine Leukocidin toxin (PVL...

  3. Maja kui diagnoos ja kommentaar = House as a diagnosis and a comment / Ingrid Ruudi

    Index Scriptorium Estoniae

    Ruudi, Ingrid, 1978-

    2009-01-01

    Korterelamust Tallinna vanalinnas Aia 4 / Vana-Viru 13, hoone sobivusest vanalinna. Autorid Ott Kadarik, Villem Tomiste ja Mihkel Tüür, projekteerija AB Kosmos, konstruktor Maru Ehitus. Projekt 2003-2009, valmis 2009

  4. Muutuv kommunikatsioon kultuuri ei ohusta / Annika Poldre

    Index Scriptorium Estoniae

    Poldre, Annika

    2011-01-01

    Tallinna Konverentside poolt Sokos Hotel Virus 28. aprillil 2011 korraldatud kommunikatsiooni aastakonverentsist "Muutuv kommunikatsioon: vana ei taha ja uut ei oska. Kuidas edasi?", kus esinesid Ahto Lobjakas, Priit Põiklik, Piia Tamm ja Marju Lauristin

  5. "Kohtuniku ees seisab kaks jumalat, kaks õigust ja kibe tarvidus ühte kui teist ehk mõlemaid korraga teenida." / Olja Kivistik

    Index Scriptorium Estoniae

    Kivistik, Olja, 1989-

    2012-01-01

    20. saj. alguses kohaldatud kriminaalseadustikest Tsaari-Venemaal (ka Eestis), nendes sätestatud usuvastaste süütegude koosseisude rakendamisest. Tsaariaegse Tallinna Ringkonnakohtu lahenditest ajal, kui kehtisid samaaegselt vana ja uus nuhtlusseadustik

  6. Hekiringide ruum / Merle Kares

    Index Scriptorium Estoniae

    Kares, Merle

    2004-01-01

    Suure lagedavõitu aia saab põnevaks ja varjuliseks liigendada hekkidega. Suure-Jaanis oleva aia ümberkujundamine. Näidisprojekti koostas Merle Kares. Ill.: vana aia vaade, uus aiaplaan, sillutise näidis

  7. Norma lõikab kasu Venemaa abipaketist / Sirje Rank

    Index Scriptorium Estoniae

    Rank, Sirje, 1966-

    2010-01-01

    Venemaa valitsus käivitas kohaliku autotööstuse toetuseks vana auto uue Venemaal valmistatud auto vastu vahetamise programmi, millest loodab kasu saada ka Norma, kuna toodab tarvikuid firmale AvtoVaz

  8. Ilusad vanad majad / Ivi-Els Schneider

    Index Scriptorium Estoniae

    Schneider, Ivi-Els, 1945-

    2003-01-01

    Sisearhitekti nõuandeid neile, kel on soov soetada endale või hakata põhjalikult remontima vana maja. Illustratsiooniks Tartu linnapea Andrus Ansipi pereelamu Tammelinnas (1923, arhitekt Anatoli Podtshekajev) renoveerimise algstaadiumist valmimiseni

  9. Preemiaga pärjati meremeeste romaan / Sirje Semm

    Index Scriptorium Estoniae

    Semm, Sirje, 1950-

    2007-01-01

    Eesti Kultuurkapitali kirjanduse sihtkapitali tõlke aastapreemia sai Tartu ülikooli usuteaduskonna Vana Testamendi prof. Kalle Kasemaa Nikos Kavvadiase kreekakeelse romaani "Vahikord" (Tallinn : Olion, 2006) tõlkimise eest

  10. Soodusrenoveerimine kogub hoogu / Selene-Rete Rebane

    Index Scriptorium Estoniae

    Rebane, Selene-Rete

    2002-01-01

    Tallinna kultuuriväärtuste ameti poolt koos erafirmadega käivitatud projekti "Vana maja korda" raames restaureeritakse puitmajde uksi ning väljastatkse värvipasse, mille alusel saab soodushinnaga fassaadivärve osta

  11. Irina Starzhenetskaja Vene galeriis

    Index Scriptorium Estoniae

    1998-01-01

    26. märtsist Vene saatkonna galeriis Moskva kunstniku Irina Starzhenetskaja maalide näitus. Kunstnik eksponeerib oma viimase aja maale, kus ta on ainest leidnud vana-vene ikoonidest ja argistest natüürmortidest

  12. Открылся выставка "Нежность" художников из Беларуси и Эстонии

    Index Scriptorium Estoniae

    2010-01-01

    Tallinna Vana Wiru hotellis avati Eesti ja Valgevene kunstnike ühisnäitus "Õrnus". Näituse korraldas galerii SED Arte eesotsas Olga Ljubaskinaga. Eesti kunstnikest osaleb Svetlana Aleksejeva Tallinnast

  13. Kontserdipeegel / Igor Garshnek

    Index Scriptorium Estoniae

    Garšnek, Igor, 1958-

    1999-01-01

    T. Vana soolokontserdist Estonia kontserdisaalis 18.mail. Kavas Mozart, Beethoven, Brahms, Chopin. Eesti Kontserdi hooaja lõppkontserdist Estonia kontserdisaalis 22.mail. E. Klasi dirigeerimisel kanti ette G. Mahleri Sümfoonia nr.2 c-moll

  14. Kuidas planeerida elamisväärset korterit? / Aet Piel ; intervjueerinud Karen Jagodin

    Index Scriptorium Estoniae

    Piel, Aet, 1966-

    2011-01-01

    Sisearhitekt Aet Piel halva planeeringuga vana korteri planeeringu muutmisest ja korteri kujundamisest. Hruštšovka-tüüpi korteri ümberplaneerimise võimalustest. Muudatuste tegemisest uues korteris. Köökidest

  15. Карикатура в газете IRL возмутила еврейскую общину / Алексей Гюнтер

    Index Scriptorium Estoniae

    Гюнтер, Алексей, 1979-

    2009-01-01

    Isamaa ja Res Publica Liidu ajalehes Eesti Eest ilmunud Gori vana karikatuur solvas Eesti juudi kogukonda. Pilapilt on Gori originaallooming, võetud 2003. aastal ilmunud raamatust "Tuntud ja tundmatu Gori". Partei pressiesindaja Rauno Veri selgitused

  16. Meenutades Albert Uustulndi rahvateatri tegemistes / Rein Rooväli

    Index Scriptorium Estoniae

    Rooväli, Rein

    2005-01-01

    A. Uustulndi kalurite elu kujutavad näidendid "Traalid udus", Mere peremehed", Vana paat", "Kaugused kutsuvad", "Neptuni pärijad", Naistele mehed, meestele meri", "Soolane suudlus" on tähtsat osa etendanud rahvateatrite repertuaaris

  17. Suurde tühja ruumi mahub palju kunsti / Margus Kiis

    Index Scriptorium Estoniae

    Kiis, Margus

    2006-01-01

    "tARTu kunstikuu 2006" näitustest Tartu vanas kaubamajas. Lühidalt plakatinäitusest "Plakat kunsticool", rühmituse JAK installatsioonist "-50%", Kaisa Eiche ja Taavi Piibemanni aktifotode näitusest

  18. Ilmar Külveti mälestusteose esitlus Tartus / Riina Kindlam

    Index Scriptorium Estoniae

    Kindlam, Riina, 1969-

    2005-01-01

    7. novembril 2005 Eesti Kirjandusmuuseumis. Kogumik pealkirjaga "Vana arm ei roosteta" ilmus ajakirjaniku 85. sünnipäevaks. Raamatuesitlusel esinesid Anne Valmas, Janika Kronberg, Vaike Külvet, Piret Noorhani

  19. "Krokodillis" ilmutab end lõbus Dostojevski / Mark Rozovski ; interv. Rednar Annus

    Index Scriptorium Estoniae

    Rozovski, Mark

    2006-01-01

    Vene lavastaja ja teatrijuht Mark Rozovski kirjutas Fjodor Dostojevski pamfletist "Krokodill" teatriteksti ja lavastab seda Vana Baskini Teatris. Esietendus 28. okt. Salme Kultuurikeskuses. Lisa : 1 küsimus Eino Baskinile

  20. Tolko ljubov! / Irina Butjajeva

    Index Scriptorium Estoniae

    Butjajeva, Irina

    2008-01-01

    Tallinna Vana Viru hotelli fuajees avati maali- ja skulptuurinäitus. Korraldajaks on SED ARTE Gallery, galerist Olga Ljubaskina. 16 osaleja hulgas on Anatoli Strahhov, Sergei Jantshenko, Iris Uuk, Aivar Rihkrand, Vera Stanishevskaja, Vladimir Baciu ja skulptor Tauno Kangro

  1. Raamat sõja seadustest / Linnart Mäll

    Index Scriptorium Estoniae

    Mäll, Linnart

    2001-01-01

    Vana-Hiina sõjateoreetikute Sun Zi ja Sun Bini raamatust: Sun, Zi ; Sun, Bin. Sõja seadused / klassikalisest hiina keelest tõlkinud ja kommenteerinud Märt Läänemets. Tln. : Eesti Keele Sihtasutus, 2001. (Sõjandusvaramu. Klassika)

  2. Kaks hõbekeelset kannelt helisemas taas ... / Liina Saarlo

    Index Scriptorium Estoniae

    Saarlo, Liina, 1974-

    2012-01-01

    Eesti regilaulukogude "Vana Kannel" 9. ja 10. osa ilmumise puhul vaadatakse tagasi sarja ilmumise ajalukku - Jakob Hurda esimestest kogudest ja nende koostamispõhimõtetest alates, visandatakse ka tulevikuplaane

  3. Arendajad asuvad julgelt uute projektide kallale / Tea Taruste ; kommenteerinud Andres Teder

    Index Scriptorium Estoniae

    Taruste, Tea

    2010-01-01

    Arco Vara ehitab kortermaju Tallinnas Kodukolde elurajooni ning on alustanud Kodukolde uue etapi korterite müüki, NCC alustas Vana-Kuuli III etapi ehitust ning jätkab Pärnaõue elamuprojekti arendust

  4. Kuidas olla suurem oma saatusest / Aivar Kull

    Index Scriptorium Estoniae

    Kull, Aivar, 1955-

    1995-01-01

    Arvustus: Wallis, Velma. Kaks vana naist : Alaska legend reetmisest, vaprusest ja ellujäämisest / Tlk. Arvo Valton. Tln.: Kupar, 1995. Vaata ka: Kull, Aivar. Kulli pilk. - Tartu : Ilmamaa, 2005, lk. 49-50

  5. Eesti-Soome tandem pistab sportlastele pihku suusa-Ferrarid / Priit Pullerits

    Index Scriptorium Estoniae

    Pullerits, Priit, 1965-

    2008-01-01

    Kunagine Soome murdmaasuusataja Marko Kantaneva ja eestlanna Terje Muuli loodavad innovatiivsete Sträp-keppidega järgmise kümnendi jooksul vallutada viiendiku maailmaturust. Lisaks: Vana ja uus käerihm. Spordikeppide turg

  6. "Mikess sinilind ..." : [luuletused] / Siim Kärner

    Index Scriptorium Estoniae

    Kärner, Siim

    2002-01-01

    Sisu: "Mikess sinilind ..." ; "Üü uss vajje ..." ; "Näitsik ütsinde ..." ; "Tullive nuure ..." ; "Piläkun põlep pird ..." ; "Vana-Kuusik tõstap teretusess ...". Eluloolisi andmeid autori kohta lk. 373-374

  7. Positiivse programmiga Pidulas / Karin Paulus

    Index Scriptorium Estoniae

    Paulus, Karin, 1975-

    2006-01-01

    Saare maakonnas Kihelkonna vallas asuvast Pidula mõisast, omanik Vesa Lappalainen. Mõisa restaureerimisprojekti koostas Aet Maasik arhitektuuribüroost Vana Tallinn, sisustuskavandite autor on Leila Pärtelpoeg. Restaureerimistöid juhendas Tõnu Sepp

  8. Poljarnõi ekspress protiv Plohhogo Santõ / Jevgeni Levik

    Index Scriptorium Estoniae

    Levik, Jevgeni

    2005-01-01

    Kaks vastandlikku jõulufilmi - Robert Zemeckise animeeritud jõulumuinasjutt "Polaarekspress" ja Terry Zwigoffi must jõuluvanakomöödia "Vastik vana" ("Bad Santa"). Lisa : Näitleja Tom Hanksi mõtteid oma rollide kohta "Polaarekspressis"

  9. Bussijaama bluus Helsingis / Harry Liivrand

    Index Scriptorium Estoniae

    Liivrand, Harry, 1961-

    2006-01-01

    Kohaspetsiifiline näitus "Saapumisen ja lähtemisen paikat" (Saabumise ja lahkumise kohad) Helsingi vanas bussijaamas ja Kamppi keskuses, kuraator Sirje Helme. Pikemalt Jouko Lehtola ja Helena Sandmani fotodest

  10. Sisyphose pruut ja teised libaolendid / Reet Varblane

    Index Scriptorium Estoniae

    Varblane, Reet, 1952-

    2011-01-01

    Terje Ojaveri loomingust : autoportreeline video "Sisyphose pruut" (2009), performatiivne teos "Insignia" (2000), videoinstallatsioon "Hoia end" (2002), installatiivne objekt "Vana" (2005) ning naisfiguurid "Sõbra õlg", "Pime", "Nägija", "Naine lemmikloomanahast kraega" (2011)

  11. Mudaravila kinnistu arendajaks Alvarelli Invest

    Index Scriptorium Estoniae

    2007-01-01

    Pärnu mudaravila kinnistu 50-aastasest hoonestusõigusest oli huvitatud Andres Tiigi firma Alvarelli Invest. Firma peab 2009. a. suveks vana mudaravila restaureerima ja rajama sellele mere poole juurdeehitise

  12. Alte Harfe. Vollständige Sammlung alter estnischer Volkslieder : Vorrede / Jakob Hurt

    Index Scriptorium Estoniae

    Hurt, Jakob, 1839-1907

    2005-01-01

    Eessõna rmt. : Hurt, Jakob. Vana kannel. 1. kogu : täieline kogu vanu eesti rahvalaulusid = Alte Harfe : vollständige Sammlung alter estnischen Volkslieder. Tartu, 1875-1886. - (Eesti Kirjameeste Seltsi toimetised ; 3)

  13. Märkmeid uusima Preisi tsensuuriinstruktsiooni kohta / Karl Marx

    Index Scriptorium Estoniae

    Marx, Karl

    2000-01-01

    Preisi uue (1841. aasta) tsensuuriinstruktsiooni tõusikliku vaimu vastuolust vana (1818. aasta) tsensuurimääruse ratsionalismiga. Moraali ja religiooni vastuolust. Vt. ka Ain Kaalepi artiklit "Veel Karl Marxist ja tsensuurist

  14. Morrise müstiline maailm / Jaanika Palm

    Index Scriptorium Estoniae

    Palm, Jaanika, 1973-

    2008-01-01

    Retr. rmt.: Birck, Jan. Salaagent Morris ja vana tuuleveski saladus : põnevik piltides ; Salaagent Morris : hommikusöök võib oodata! : põnevik piltides (saksa keelest tõlkinud Kaja Ziegler. Tallinn : Grenader, 2007)

  15. Sardiinia kirjandus kui hea idee

    Index Scriptorium Estoniae

    2007-01-01

    Toimetuse artikkel Elsbeth Gut Bozzetti artikli "Vana maailm ja uus laine" (Alte Welt und neue Welle. - Neue Zürcher Zeitung 16.12. 2006) ning internetis avaldatud materjali põhjal. Gavino Ledda, Salvatore Satta, Salvatore Niffoi jt.

  16. Новый корпус средней школы, Вильянди = Extension of Viljandi Secondary School / Михкель Кару

    Index Scriptorium Estoniae

    Кару, Михкель, 1983-

    2013-01-01

    Viljandi Riigigümnaasiumi uuest hoonest. Arhitektid Maarja Kask, Karli Luik, Ralf Lõoke, Andro Mänd, Margus Tamm (Salto Arhitektid), sisekujunduse autor Pille Lausmäe. Vana maja fassaadi renoveerimisprojekti autor Eva Laarmann

  17. Rootsi rangus kimbutab Tallinkit lisanõuetega / Lauri Linnamäe

    Index Scriptorium Estoniae

    Linnamäe, Lauri

    2007-01-01

    Rootsi mereinspektorid leidsid puudusi veekindlate uste elektrisüsteemides Tallinki laevadel Regina Baltica ja Vana Tallinn ning Viking Line'i Mariellal, kirjutab Rootsi meedia. Vt. samas: Galaxy vahetab lippu

  18. Taastatava kiriku alla tuleb krüpt ja kolumbaarium : Pauluse kirik valmistub suureks remondiks / Vilja Kohler

    Index Scriptorium Estoniae

    Kohler, Vilja, 1966-

    2008-01-01

    Tartu Pauluse kiriku (arhitekt Eliel Saarinen) remondist. Restaureerimise arhitektuurikonkursi võitis soome arhitektide Merja Niemise, Kari Järvise ja Markku Norsi töö "Eli El". Vana krohvikorra all peidus olnud seinamaalingutest. Kommenteerib Priit Humal

  19. Viis raamatutäit poola näidendeid / Riina Mägi

    Index Scriptorium Estoniae

    Mägi, Riina, 1957-

    2006-01-01

    Tõlkija Hendrik Lindepuult ilmus viies tõlkekogumik poola dramaturgia tippteoseid tutvustavast sarjast. Seekord said kaante vahele Tadeusz Rozewiczi näidendid "Valge abielu", "Lõks" ja "Vana naine haub"

  20. Eesti rohelised juured: vitsik, pütt ja lännik polikliinikus / Eha Järv

    Index Scriptorium Estoniae

    Järv, Eha, 1954-

    2010-01-01

    Avinurme puidutoodete firmast E. Strauss, kus tehti korda autori kolm vana puunõud. Lisa: Väike illustreeritud vitsikute entsüklopeedia (toober ja vann, ankur, pesupali, astja, tõrs, kapp ja kibu, lännik)

  1. Mennoniidid - alternatiiv rahvusriigile : vestlus Tallinna ülikooli antropoloogiaprofessori Lorenzo Cañás Bottosega / Lorenzo Cañás Bottos ; küsitles Maarja Kaaristo

    Index Scriptorium Estoniae

    Cañás Bottos, Lorenzo

    2008-01-01

    Lorenzo Cañás Bottos avaldas oma Manchesteri ülikoolis kaitstud doktoritöö ingliskeelse monograafiana "Vana koloonia mennoniidid Argentinas ja Boliivias. Riigiloome, religioossed konfliktid ja tuleviku kujutlemine"

  2. Kes kardab Park Chan-Wooki? / Karlo Funk

    Index Scriptorium Estoniae

    Funk, Karlo, 1971-

    2007-01-01

    Vägivalla ajendite teemadel. USA meedia süüdistas peale Virginia koolitulistamist filmikunsti. Seoseid ja/või põhjusi otsiti korealase Park Chan-Wooki filmist "Vana poiss" ("Old Boy") ja John Woo filmidest

  3. Интеграция бывает и виртуальной / Алина Зелимханова

    Index Scriptorium Estoniae

    Зелимханова, Алина

    2005-01-01

    Projektist "AVAsta", milles osalevad Tallinna Kesklinna Vene gümnaasiumi, Vana-Kalamaja Täiskasvanute Gümnaasiumi ja Sindi Gümnaasiumi õpilased. Projekti sisuks on eesti ja vene õpilaste integratsioon ja keeleõpe

  4. Presence of Glycopeptide-Encoding Plasmids in Enterococcal Isolates from Food and Humans in Denmark

    DEFF Research Database (Denmark)

    Migura, Lourdes Garcia; Valenzuela, Antonio Jesus Sanchez; Jensen, Lars Bogø

    2011-01-01

    developed techniques for classification of plasmids. Replicons associated with sex pheromone-inducible plasmids were detected in all GR E. faecalis, whereas GR Enterococcus faecium contained plasmids known to be widely distributed among enterococci. vanA resistance is common in E. faecium isolates from meat...... and animals in Europe and is rarely found in E. faecalis. This article describes the first characterization of MGE from vanA mediated E. faecalis, thus linking this resistance genotype to pheromone responding plasmids....

  5. Molecular characterization of van genes found in vancomycin-resistant Enterococcus spp. isolated from Hospital das Clínicas, FMUSP, São Paulo, Brazil

    Directory of Open Access Journals (Sweden)

    H.H. Caiaffa Filho

    Full Text Available Vancomycin-resistant enterococci strains (VRE is an important pathogen related with hospital infections in many countries, presenting limited or no therapeutic options for treating serious infections. VRE has presented some different genotypes been VanA and VanB considered to be the most important in hospital environments. In the present study the authors investigated the prevalence of van genes (A, B an C among clinical isolates of VRE in a five month period at a large tertiary hospital in Sao Paulo, Brazil. The results showed the presence of vanA, but not vanB or vanC in all 43 strains of E. faecalis and five E. faecium studied. The results bring an important issue, due to the possibility of resistance spread of vanA genes, to be monitored and solved by the hospital infection control team and the microbiology and molecular biology laboratories at tertiary Hospitals.

  6. Nasha zhizn stanovitsja eksperimentom / Virkko Lepassalu

    Index Scriptorium Estoniae

    Lepassalu, Virkko, 1971-

    2007-01-01

    Venemaa üks tuntuim teisitimõtleja Sergei Haritonov peab Sosnovõi Bori tuumajaama puhul ohtlikuks korrumpeerunud ja mittekvalifitseeritud tööjõudu ning radioaktiivsete jäätmete basseine tuumajaama juures. Kuritarvitustest Sosnovõi Bori tuumajaamas, probleemidest seoses vana reaktori demonteerimise ja uute energiablokkide rajamisega

  7. Härra viina maailmarännak / Raul Ranne

    Index Scriptorium Estoniae

    Ranne, Raul

    2005-01-01

    AS Liviko kasutab toorainena USA-st ostetud viina. Liviko juht Udo Themas ja turundusdirektor Janek Kalvi põhjendavad toorme sissetoomist kohaliku piirituse madala kvaliteediga. Võimalikust skeemist, kuidas toorainet konkurentsivõimelise hinnaga kätte saada. Skeem: Viin rändab kui vana aja maadeavastajad

  8. Mõõtke kulusid õigesti - tehke õigeid otsuseid / Robin Cooper, Robert S Kaplan, Leonard L Berry...[jt.

    Index Scriptorium Estoniae

    2001-01-01

    Tegevuspõhise kuluarvestussüsteemi tutvustus. Kulude jaotumine tegevuspõhist kuluarvestussüsteemi arvesse võttes. Tabelid: Kuidas tegevuspõhine kuluarvestus muudab toote kasumlikkust; kasumi jagunemine kommerts-turustuskanalite kaupa: vana ja uus süsteem

  9. Kuressaare 2008. aasta parimad ehitised selgunud / kommenteerinud Hannes Koppel

    Index Scriptorium Estoniae

    2009-01-01

    Parim terviklahendus golfiala (golfiväljaku projekteerija soome arhitekt Lassi Pekka Tilander, klubihoone projekteeris arhitektuuristuudio Siim & Kreis arhitekt Andres Siim). Parim uusehitis eramu Merikotka 45 (projekteerija Ösel Plan arhitekt Terje Truumaa) ja parim renoveeritud ehitis vana pritsumaja (Kuressaare Kommunaalprojekt projekteerija Galina Kanemägi)

  10. "Nu, pogodi!" vozvrashtshajetsja / Tatjana Rostova

    Index Scriptorium Estoniae

    Rostova, Tatjana

    2005-01-01

    Populaarsele vene animafilmile "Oota sa!" ("Nu, pogodi!") valmis stuudios Christmas Films uus, 19. osa, mis tuleb ekraanile novembris. Filmi stsenarist on Aleksandr Kurljandski ja režissöör vana multifilmi tegija režissöör Vjatsheslav Kotjonotshkini poeg Aleksei

  11. Pahaendeline pragmatism / Enn Soosaar

    Index Scriptorium Estoniae

    Soosaar, Enn, 1937-2010

    2008-01-01

    Vana Maailma kõlbeline kollaps oli see, mis lasi Euroopas tekkida ja võimust võtta XX sajandi totalitaarsetel režiimidel. Ohtu peame tajuma siis, kui huvide taha peidetakse riigi imperialistlikud ambitsioonid või merkantiilne saamahimu. Ilmunud ka Lääne Elu 18. okt. lk. 2

  12. Tallinna 21. kool : Tallinn, Raua tn. 6 = Tallinn School No. 21 : Raua Street 6, Tallinn / Piret Lindpere

    Index Scriptorium Estoniae

    Lindpere, Piret, 1963-

    2003-01-01

    1923. a. valminud koolihoone renoveerimine ja juurdeehitus.Vana ja uut maja ühendab klaaskatusega läbi korruste paiknev aatrium. Projekteerija: Raul Järg, AS Kommunaalprojekt. Autor Raul Järg. Sisekujundaja Toomas Korb (Pink). Konstruktsioonid: AS Kommunaalprojekt, OÜ Civen. Projekt 2002, valmis 2003. Uue osa I ja II korruse plaan, 7 vaadet

  13. Tartu Ülikool Narva linna vastu / Jüri Kuuskemaa

    Index Scriptorium Estoniae

    Kuuskemaa, Jüri, 1942-

    2007-01-01

    Tartu Ülikooli Narva Kolledži uue hoone projektist ja paiknemisest, selle vastuoludest muinsuskaitsega ning Narva linna sooviga taastada Raekoja platsi hoonestus ajaloolisel kujul. TÜ Narva Kolledži uue hoone projekt viis Ülikooli kohtusse. Fotodel vaateid vana Narva Raekoja platsist

  14. Männiku sotsiaalelamu = Social housing in Männiku / Peeter Pere

    Index Scriptorium Estoniae

    Pere, Peeter, 1957-

    2012-01-01

    2012. a. Tallinnas Männiku tee 92 valminud inva- ja peremajutamiseks mõeldud sotsiaalelamu, vana ühiselamu ümberprojekteerimine. Arhitektid: Siiri Vallner, Indrek Peil, Kaire Nõmm, kaasautorid: Ragnar Põllukivi, Andro Mänd, Kadri Klementi (Kavakava). Sisearhitekt Tarmo Piirmets (Pink). Projekt: 2010-2011

  15. Pustõr za Pevtsheskim polem vernjotsja k zhizni / Urmas Tooming

    Index Scriptorium Estoniae

    Tooming, Urmas

    2006-01-01

    Vana-Kuuli elurajooni rajamisest Tallinnas Lasnamäe linnaosas. Kavas on kolme 8-korruselise elamu ja kahe parkimismaja ehitamine ning ühe paekiviehitise renoveerimine. Kvartali detailplaneeringu koostas ja maju projekteerib Kalle Rõõmuse arhitektuuribüroo. Kommenteerivad Andres Madalik, Vello Alurand ja Kalle Rõõmus

  16. Tallink Silja AB juht Kadri Land : eelistan entusiastlikke eksimusi lõputule ja laisale halale / Kadri Land ; interv. Andres Reimer

    Index Scriptorium Estoniae

    Land, Kadri

    2008-01-01

    Tallink Silja AB juht vastab küsimustele, miks Tallink ei huvitu Sillamäe-Kotka või Saaremaa laevaliinist, mille poolest erineb Tallinki juhtimismeetod vana Silja omast, mida on eestlastel rootslastele õpetada, kas meie turismisektor suudab Eestit rootslastele ahvatlevaks muuta, miks Tallink Silja pole veel avanud Stockholm-Peterburi liini. Diagramm: Tallink Grupp

  17. Linnast maale ja edasi = From city to country and beyond / Jaan Tiidemann

    Index Scriptorium Estoniae

    Tiidemann, Jaan

    2012-01-01

    Tähevälja eramu (593 m²) Kiiu-Aabla külas Lahemaal. Vana puhkebaasi hoonete rekonstrueerimine. Autor ja projekteerija Martin Aunin. Sisearhitekt Tiit Jürna. Vaatetorn: Martin Aunin, Martin Melioranski. Arhitektuurivõistlus: 2009, projekt: 2011, valmis: 2013. Hoone sobivusest rannakülla

  18. Suvelõpu performance Karepal / Hannes Varblane

    Index Scriptorium Estoniae

    Varblane, Hannes, 1949-

    2009-01-01

    15. augustil 2009 Karepal Richard Sagritsa majamuuseumis toimunud performance'ist "Vana riist", milles osalesid Hannes Varblane, Jüri Ojaver, Alex Plutzer, Ilja Sundelevitš, Jaan Paavle, Üllar Saaremäe, Leonhard Lapin, väljapanekutega lisandusid Valeri Vinogradov ja Jaan Toomik. Projektijuht Teet Veispak

  19. Restoran Museum = Museum Restaurant

    Index Scriptorium Estoniae

    2009-01-01

    Tallinnas Vana-Viru 14 asuva restorani Museum sisekujundusest. Sisearhitekt Toomas Korb, tema tähtsamate tööde loetelu. Varem paiknes nendes ruumides tuletõrjemuuseum, endiseid asukaid meenutavad raamitud mustvalged fotod. Ruumi ilmestavad Tom Dixoni loodud kuulvalgustid

  20. Tagasivaade veebruarikuu tegevusele Ehatares / Linda Püssa ; fotod: Linda Püssa

    Index Scriptorium Estoniae

    Püssa, Linda

    2009-01-01

    tegevusjuhi Rosemarie Lindau sünnipäevast, sõbrapäevast, EV aastapäeva tähistamisest Ehatare kirikusaalis ja Vana-Andrese kirikus, Inna ja Aadu Randpalule Eesti Punase Risti III klassi teenetemärgi andmisest

  1. Üits pilt : [luuletused] / Milvi Panga

    Index Scriptorium Estoniae

    Panga, Milvi

    2002-01-01

    Sisu: Üits pilt ; Latsõ ja pini lugu ; Laut ; Jahvikivi ; Vanaesäle ; Valuvahtsõnõ ja verinuur ; Ku saat säänest üskä võtta ; Lugu juurildõ puust ; Lepp ; Piknõ. Eluloolisi andmeid autori kohta lk. 375

  2. Pärnu sanatooriumi 'Tervis' kultuurikompleks. Pärnu, Seedri 6 / Siim Sultson

    Index Scriptorium Estoniae

    Sultson, Siim

    1998-01-01

    Sööklakompleksi (vanas hoones) ja kultuurikeskuse (uues hoones) rekonstrueerimine. Kultuurikeskuses ja selle baaris panid kujundajad kõige rohkem rõhku valgustusele. Kasutatud on rohkesti peidetud hajutatud valgustust koos suunatud valgusega. Projekteerija: Stuudio GaDIS. Sisekujundajad Helle ja Taevo Gans. Ehitaja: Eston AS, AS Jart. Projekt alates 1996, teostus alates 1997.

  3. Planeerimine algab kultuuriloo tundmisest / Riin Alatalu

    Index Scriptorium Estoniae

    Alatalu, Riin, 1968-

    2011-01-01

    Viljandimaal Õisu mõisas 10.11.2011 toimunud seminarist "Kultuuriloost lähtuv planeerimine". Ettekannetest, 1. juunil jõustunud muinsuskaitseseaduse muudatustest. Õisu mõisa tulevikuplaanidest. Uuringute ja projektidega tegeleb OÜ Vana Tallinn, pargiga OÜ Artes Terrae

  4. Suur alternatsioon Pärnus

    Index Scriptorium Estoniae

    1999-01-01

    18. juunil kunstiüritus "Suur alternatsioon'99" Pärnus. Peakorraldaja Taje Tross. Pärnu vanal turuplatsil esinesid poeedid Märka, Ruitlane, Contra, ansamblid Safan Sih, Crying Harmony. Pärnu jõel vana silla juures toimus Sorge klaverikontsert. Academia Non Gratas avati nongratalaste näitus.

  5. "Minagi olin Arkaadia teel..." / Juhan Maiste

    Index Scriptorium Estoniae

    Maiste, Juhan, 1952-

    2008-01-01

    Pargist kui mõtlemise kahest kategooriast (Platon ja Aristoteles), selle semiootilisest tähendusest alates tsivilisatsiooni algusest, parkide ajaloost vanas Kreekas, Roomas, Araabias ja Euroopas 19. sajandini, ka mõisatest ja mõisaparkidest kui kultuurinähtusest Baltimaadel

  6. Kunstnikuks pühitsemine Hype Gallery's / Margit Tõnson

    Index Scriptorium Estoniae

    Tõnson, Margit, 1978-

    2004-01-01

    Londonis Old Truman Brewery galeriihoones Hewlett Packardi toetusel kunstiprojekt kuni 28. veebrarini 2004, mille raames loomingulised inimesed saavad end määratleda kunstnikuna. Ainus limiteeriv kriteerium on tähtede HP olemasolu pealkirjas. Kübergaleriisse saadetud töö prinditakse ja riputatakse vana õlletehase seintele

  7. [Lexikon des alten Handwerks. Vom späten Mittelalter bis ins 20. Jahrhundert] / Raimo Pullat

    Index Scriptorium Estoniae

    Pullat, Raimo, 1935-

    2009-01-01

    Arvustus: Lexikon des alten Handwerks. Vom späten Mittelalter bis ins 20. Jahrhundert. Hrsg. von Reinhold Reith. München, 1991. Leksikoni autorid on piirdunud geograafiliselt saksa keeleruumiga. Kirjeldatud on peamiselt neid elukutseid, mis kajastavad nn vana käsitöö töö- ja elulaadi põhielemente.

  8. Elav monument : [luuletused] / Johannes Vares-Barbarus

    Index Scriptorium Estoniae

    Barbarus, Johannes, pseud., 1890-1946

    1998-01-01

    Sisu: Elav monument ; Multiplitseerit hommik ; Hall reisikiri ; Põrsad ; Värssjutt surijast tütarlapsest ; Tulipunkt ; Ämblik koob võrku... ; Luule klassivõitlus ; Nurgakivi panek ; Maja tellingutes ; Vana tuulik ; Värss rajus ; Dionysos ; Klassika ; Pürgimus ; Leningrad kevadõites ; Jälle kodupinnal ; Ühe kaotuse puhul

  9. Journal of Applied Sciences and Environmental Management - Vol ...

    African Journals Online (AJOL)

    ... impacted with crude oil · EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT ... of changes in Aerial Extent of Lake Chad using Satellite Remote Sensing Data ... Determination of vehicular pollution in the road tunnel of Vana (Haraz Road) in ...

  10. Tallinna Tehnikakõrgkooli Arhitektuuri Instituudi rahvusvaheline töötuba 10.-14. november 2008

    Index Scriptorium Estoniae

    2009-01-01

    Õpikoja "Uus elu vanas kestas" juhendajad: arhitektuuriteadlane Anke Zalivako (Saksamaa), arhitekt Irina Raud. Osalesid 3. kursuse arhitektuuri eriala tudengid. Tuli leida uus kasutusfunktsioon endisele gaasihoidlale Tallinnas Põhja puiesteel ja veetornile Ülemiste City-s, võimalus Nõmmel Nurme t. 40 asuva villa (arhitekt E. J. Kuusik, 1935) taastamiseks esialgselt kavandatud kujul

  11. Commensuration and policy comparison: How the use of standardized indicators affects the rankings of pension system / Hans Peeters, Gert Verschraegen, Annelies Debels

    Index Scriptorium Estoniae

    Peeters, Hans

    2014-01-01

    Artikkel käsitleb standardiseertud indikaatorite konstrueerimist ja kasutamist pensionipoliitikas, mis aitavad võrrelda ja hinnata eri OECD liikmesriikide pensionisüsteemide sotsiaalset tulemuslikkust, nt vaesusriski vähendamine või elustandardi säilitamine vanas eas. Teiste seas on vaatluse all ka Eesti. Joonised

  12. Archaeological excavations on the plot of the old department store in Tartu, Riia Street 2 / Rünno Vissak, Eero Heinloo, Silja Möllits

    Index Scriptorium Estoniae

    Vissak, Rünno, 1962-

    2015-01-01

    Vana Kaubamaja kvartali kasutus intensiivistus 14. saj. lõpus, mil uuendati olemasolevaid ja rajati uusi piirikraave. Lühiajaline paus piirkonna asustuses toimus Vene-Liivi sõja ajal. 17. sajandil tastatud puithoone viitab asustuse järjepidevusele. Hiljemalt aastaks 1732 on kinnistule ehitatud uus puithoone, mis püsib kasutusel vähemalt 19. sajandi alguseni

  13. Üleajateenijate elamu vaated, u 1935 = Soldier's quarters, ca 1935 / Roman Koolmar

    Index Scriptorium Estoniae

    Koolmar, Roman, 1904-1971

    2015-01-01

    Lasnamäe lennubaasi elumajad valmisid ca 1936 (projekt 1935). Välisvaated Tallinnas Narva mnt ja Vana-Kuuli tänava nurgal paiknenud üleajateenijate ja ohvitseride elumajadest, korruseplaanid. Hooned on lammutatud. Lk. 49 aerofoto lennubaasist 1930-ndate lõpus

  14. Antonio Machado y Ruiz (1875-1939) / koost. ja tlk. Andres Ehin

    Index Scriptorium Estoniae

    2006-01-01

    Hispaania luuletajast Antonio Machadost. Sisaldab ka autori luuletusi "Vanasõnad ja laulud XXIX", "Kas mu süda on uinunud?", "Kõrgmaa laulud", "Tuul, toreda päeva tuul", "Eile öösel nägin und", "Neli luuletust"

  15. Valge loss Pedja kaldal : [Puurmani loss] / Meeli Müüripeal

    Index Scriptorium Estoniae

    Müüripeal, Meeli, 1963-

    2010-01-01

    Puurmani lossi restaureerimine hakkab lõpule jõudma. Maja avamispidu on novembris 2010. Restaureerimiskontseptsiooni ja põhiprojekti koostas Anu Kulbach, ehitaja AS Tevo, restaureerijad OÜ Vana Tallinn, Tartu Kõrgem Kunstikool, esimese korruse sisekujundus AS Restor, autor Reet Saluvere

  16. Ohtlik enesetsensuur? / Reet Varblane

    Index Scriptorium Estoniae

    Varblane, Reet, 1952-

    1999-01-01

    18.-20. VIII Pärnus peetud konverentsist "Tsensuur ja enesetsensuur", mis keskendus totalitaristliku tsensuuri headele ja halbadele külgedele, enesealgatusliku tsensuuri tekkimisele vabaturu tingimustes ja enesetsensuurile vanas ja uues olukorras. Ettevõtte algataja M. Soosaar. J. Vidal-Halli, E. Lucie-Smithi, M. Lõhmuse, P. Linnapi, L. Lapini ja M. Soosaare ettekannetest

  17. Meie maa - Rehemaa / Terje Alas

    Index Scriptorium Estoniae

    Alas, Terje

    2005-01-01

    Aiakujundaja Toomas Türk ning käsitööõpetaja ja aiakujundaja Terje Alas on kolinud oma perega elama Raplamaale Rehemaa tallu ja vahendavad vana talu elluäratamist oma uueks koduks. Talukoha valikust, hoonetest, aiast ja esimestest sammudest aia uuendamisel. 7 ill

  18. Karjamõisast käsitöökojaks / Helju Koger

    Index Scriptorium Estoniae

    Koger, Helju, 1943-

    2009-01-01

    Sulu karjamõisa ajaloost ja omanikest. Mõisa renoveerisid ja moodustasid MTÜ Sulu Käsitöökoda soomlased Hannele ja Juha Paunonen. Restaureeriti vana tellisepõletusahi ja ehitati keraamikaahi, eesmärgiks käsitööoskuste edendamine külas

  19. Eesti arhitektuuriuudised / Aale Kask

    Index Scriptorium Estoniae

    Kask, Aale

    2007-01-01

    "Eesti betoonehitis 2006" preemia saanud Hindrek Kesleri projekteeritud Luku-Experdi maja Tartu maanteel (Büroo AB Zero), Aasa külalistemajaks Raivo Mändmaa poolt renoveeritud vana maja Viljandis, skulptor Jass Kaselaane kavandite järgi valminud maasikad Viljandi linnas, mis tähistavad teed Paul Kondase keskusesse, põhumaja Nina külas

  20. 7.-16. juunini toimub Eesti Kunstiakadeemia skulptuuri õppehoones...

    Index Scriptorium Estoniae

    2004-01-01

    Rahvusvaheline malmivalu sümpoosion. Osalevad Helsingi Kunstiakadeemia, East-Carolina Ülikooli (USA), Buffalo State College'i (USA), Gerrit Rietveld Academie (Holland) ja Lissaboni Ülikooli skulptuuriosakonnad. Juhendavad Carl Billingsley, Kenneth Payne, Villu Jaanisoo, Jaak Soans. Valminud töödest avatakse näitus Vana Dominiiklaste kloostris

  1. "Welcome to Estonia" tuleb kinolinale / Maria Ulfsak

    Index Scriptorium Estoniae

    Ulfsak, Maria, 1981-

    2002-01-01

    Poliitikuid pilavad Rain Tolk ja Ken Saan uues filmis "Welcome To Estonia" : režissöör Andres Maimik. Film esilinastus 6. detsembril kinos Sõprus Pimedate Ööde filmifestivali raames. Kanal2 näitab seda vana-aastaõhtul

  2. Kulturu delajut na zavodah / Alina Zelimhanova

    Index Scriptorium Estoniae

    Zelimhanova, Alina

    2005-01-01

    Kultuuritehase projektijuht Madis Mikkor Tallinnas Põhja puiesteel asuvast muinsuskaitse all olevast elektrijaama katlamajast, kuhu peatselt kolib kultuuritehas Polymer. Katlamaja plaanitakse avada augustis. Selleks ajaks seatakse sisse valgustus. Hoonete põhjalikum renoveerimine algab 2007. a., kusjuures säilitatakse vana tööstusmiljöö

  3. Põrgulikud piinad ja tingimusteta armastus / Margit Tõnson

    Index Scriptorium Estoniae

    Tõnson, Margit, 1978-

    2004-01-01

    Helsingis 17. korda toimuva festivali "Armastus ja Anarhia" kavas olnud filme - Takashi Miike "Üks vastamata kõne" ("One Missed Call"), korealase Park Chan-Wooki "Vana poiss" ("Old Boy") ja Jean-Luc Godardi "Meie muusika" ("Notre Musique")

  4. Ita Ever saab pojalt kingiks filmi / Ada Maltseva

    Index Scriptorium Estoniae

    Maltseva, Ada, 1970-

    2006-01-01

    Näitleja Ita Everi 75-ndaks sünnipäevaks jõuab ekraanile mängufilm "Vana daami visiit" : stsenaristid Roman Baskin, Ilmar Raag : režissöör Roman Baskin : MTÜ Kell Kümme koostöös ETV ja Exitfilmiga, Kultuurkapital. Filmi võtteplatsil toimunust

  5. Miljardär Ita Ever / Einar Ellermaa

    Index Scriptorium Estoniae

    Ellermaa, Einar, 1960-

    2006-01-01

    Näitleja Ita Everi 75. sünnipäevaks esilinastus mängufilm (või telelavastus) "Vana daami visiit" : režissöör Roman Baskin : stsenaristid Roman Baskin, Ilmar Raag : peaosades Ita Ever ja Aarne Üksküla

  6. No Bananas! / Eiki Nestor

    Index Scriptorium Estoniae

    Nestor, Eiki, 1953-

    2008-01-01

    Riigikogu SDE fraktsiooni esimees viitab asjaolule, et Eestis sotsiaalkaitseks tehtavate kulude osakaal on natuke üle 12%, EL-i keskmine aga 26%. Tema sõnul peab üks vana hea EL-i liikmesriik kõrgeid sotsiaalkulutusi ja töötasusid eelisteks, mitte puudusteks

  7. Kuus, kes kuidagi kokku ei saa / Veiko Märka

    Index Scriptorium Estoniae

    Märka, Veiko, 1964-

    1999-01-01

    Arvustus: Merca [Jääger, Merle]. Vana libu hommik; Ruitlane, Olavi. Inemise sisu; Contra [Konnula, Margus]. Päike ja lamp; Ilves, Aapo. Üks pedajas; Pai, Tiina. Armastab mõrvatud haldjaid; Tamme, Villu. Sorlimeki plät. Kõik : Tartu : Eesti Kostabi $eltsi Kaasaegse Kirjanduse Keskus, 1998

  8. Lutheri mööblivabriku renoveerimine korterelamuks = Renovation of the Luther furniture factory into an apartment building / Margit Mutso

    Index Scriptorium Estoniae

    Mutso, Margit, 1966-

    2009-01-01

    Tallinnas Vana-Lõuna 39 ja 39a asuva hoone (Aleksei Bubõr, Nikolai Vassiljev, 1912) muinsuskaitse eritingimused koostas Monika Eensalu, vastutav arhitekt Rasmus Tamme, arhitektid Andrei Pleskatšov, Jaanus Saarepera ja Evelin Eelma, sisearhitektid Reio Raudsepp, Eero Jürgenson (projektijuht) ja Liina Kink. Žürii hinnang kultuurkapitali aastapreemiale esitatud hoonele

  9. Der Wiederaufbau Narvas nach 1944 und die Utopie der "sozialistischen Stadt" / Karsten Brüggemann

    Index Scriptorium Estoniae

    Brüggemann, Karsten, 1965-

    2004-01-01

    Nõukoguliku arhitektuuripoliitika põhimõtetest, "sotsialistliku linna" teoreetilisest ideaalist ja selle ideaali praktilisest teostusest Narvas. Narva vanalinna saatusest. 1944. aastal asuti vana Narva kohale rajama sisuliselt uut linna, Narva ehitamist mõjutas eelkõige asjaolu, et seal sai alguse uraanitootmine NSV Liidus

  10. Prantsuse külastiilis kööginurk / Tertit Linturi

    Index Scriptorium Estoniae

    Linturi, Tertit

    2004-01-01

    Lahtine kööginurk vana eramu katusealusesse ehitatud elutoas (9 mø). Kööginurka eraldab muust ruumist kõrge töölett. Köögikapid on roheliseks värvitud puitkiudplaadist ustega, tööpinnad õlitatud massiivtammepuidust. 5 ill

  11. Punase diivani kodu / Mari-Liis Raudjärv

    Index Scriptorium Estoniae

    Raudjärv, Mari-Liis

    2008-01-01

    Sisearhitekt Maarja Mikkini ja kujundusgraafik Dan Mikkini läbi kahe korruse kulgev korter (114 m2) Tallinna kesklinnas. Kaht korrust eraldab klaaspõrand. Korter on sisustatud vana ja uue mööbliga, elutoas on firma Mang punane diivan. Suurt rõhku on pandud valguslahendusele. 13 värv. vaadet

  12. Comparison of eight methods to detect vancomycin resistance in enterococci

    NARCIS (Netherlands)

    H.P. Endtz (Hubert); N.P.W.C.J. van den Braak (Nicole); A.F. van Belkum (Alex); W.H.F. Goessens (Wil); D. Kreft; A.B. Stroebel; H.A. Verbrugh (Henri)

    1998-01-01

    textabstractA collection of genetically unrelated vancomycin-resistant enterococci (VRE) including 50 vanA, 15 vanB, 50 vanC1, and 30 vanC2 VRE were used to evaluate the accuracy of eight currently available susceptibility test methods (agar dilution, disk diffusion,

  13. Links of the population of western Finland and Estonia with the central Ilmen region in the context of international trade in the Baltic Sea region in the 9th-10th century / Evgeny N. Nosov, Natalia V. Khvoshchiskaya

    Index Scriptorium Estoniae

    Nosov, Evgeny N.

    2006-01-01

    Ilmeni piirkonnast (peamiselt Rjuriku linnusest) leitud soomeugri maailma erinevate piirkondadega seotud leidudest. Vaadeldavad leiud liikusid mööda Rootsit, Lääne-Soomet, Põhja-Eestit, Vana-Laadogat ja Volhovi ülemjooksu ühendavat teed, mis on teada 13. saj. kirjalikest allikatest. Kõnesoleval kaubamagistraalil etendas erilist kohta Iru linnus

  14. Interventsiooni paratamatus kaheksa aastat hiljem = The Inevitability of Intervention Eight Years Later / Epp Lankots

    Index Scriptorium Estoniae

    Lankots, Epp, 1976-

    2008-01-01

    Uue ning vana arhitektuuri koostoimimisest Eestis. Näiteks toodud hotelli "Telegraaf" juurdeehitus (arhitekt Martin Aunin), nelikmaja (AB Kosmos, 2007) Rotermanni kvartalis, Fahle Maja (KOKO Arhitektid, 2002-2006) Tallinnas, Pärnu kesklinna koolide võimla (Kavakava, 2004-2006), TÜ Narva Kolledzhi 2006. a. arhitektuurikonkursi võidutöö (arhitektid Siiri Vallner, Katrin Koov, Indrek Peil). Bibliograafia lk. 19

  15. Metro Plaza za fasadom Rotermanna / Viktoria Jürmann

    Index Scriptorium Estoniae

    Jürmann, Viktoria

    2008-01-01

    Pandi nurgakivi Tallinnas Viru väljaku äärde rajatavale büroohoonele Metro Plaza. Säilitatakse vana fassaad, mille tõttu kujuneb hoone Viru väljaku ja Mere puiestee poolsel küljel kaetud galerii jalakäijatele. Projekteerisid Raivo Kotov ja Kaur Stöör

  16. Vene draamateatri maja avatakse mais endisaegses hiilguses / Andres Laasik

    Index Scriptorium Estoniae

    Laasik, Andres, 1960-2016

    2006-01-01

    Vene draamateatri, endise esinduskino "Gloria Palace'i" rekonstrueerimisest ja juurdeehitusest. Saali rekonstrueerimistöid teeb OÜ Vana Tallinn koostöös KAR-Grupiga, juurdeehituse projekteeris arhitekt Ra Luhse büroost Luhse ja Tuhal. Läti arhitekti Fridrihs Skujinsi projekteeritud "Gloria Palace" valmis 1926. a. Kommentaar "Pealinna esinduskino mall võeti Riiast"

  17. B nulevoi totshke Tallina vozdvigajetsja delovoje zdanije elitnogo klassa / Lauri Leet

    Index Scriptorium Estoniae

    Leet, Lauri

    2008-01-01

    Tallinnas 2007. a. sügisest käiva Metro Plaza ärihoone ehitust tutvustab projektijuht Kuldar Gil. Vastavalt muinsuskaitselistele nõudmistele säilitati vana hoone fassaadiosa. Hoone klaasfassaadi valguskujundusest. Ehitab Facio Ehituse AS. Arhitektuurne projekt: KOKO Arhitektid OÜ. Konstruktiivse osa projekteerija: Neoprojekt. Hoone kavandamist tutvustab arhitekt Raivo Kotov

  18. Nullpunkti kerkib eliitklassi ärihoone / Lauri Leet

    Index Scriptorium Estoniae

    Leet, Lauri

    2008-01-01

    Tallinnas 2007. a. sügisest käiva Metro Plaza ärihoone ehitust tutvustab projektijuht Kuldar Gil. Vastavalt muinsuskaitselistele nõudmistele säilitati vana hoone fassaadiosa. Hoone klaasfassaadi valguskujundusest. Ehitab Facio Ehituse AS. Arhitektuurne projekt: KOKO Arhitektid OÜ. Konstruktiivse osa projekteerija: Neoprojekt. Hoone kavandamist tutvustab arhitekt Raivo Kotov

  19. Raikküla mõisa ait-kuivatist ja selle taastamisest / Karen Klandorf

    Index Scriptorium Estoniae

    Klandorf, Karen

    2014-01-01

    Täpne valmimisaeg ei ole teada, kuid 1864. aastal on mainitud seda kui vana hoonet. 1909. aastal ehitati juurde kuivati. Mõisa ait-kuivati on traditsiooniline ehitis, mille muudavad erandlikuks pikkkaaristu ja hoone katusel ilutsev kellatorn. Kuivati esindab üht levinumat kuivatitüüpi. Restaureerimistööd kestsid 2008-2013.

  20. GenBank blastx search result: AK062244 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK062244 001-047-F02 AF009672.1 Acinetobacter sp. ADP1 VanR (vanR), vanillate demethylase (vanB), van...illate demethylase (vanA), and VanK (vanK) genes, complete cds; and unknown genes.|BCT BCT 2e-14 +3 ...

  1. О знаке "на кирпичах из Киева", опубликованном Б. А. Рыбаковым / Сергей Васильевич Белецкий

    Index Scriptorium Estoniae

    Белецкий, Сергей Васильевич

    2014-01-01

    Boris Rõbakovi artiklist ajakirjas "Советская археология" 1940. a.; kolmharkmärgiga tellisel puudub seos Kiievi ja üldse Vana-Vene arhitektuurimälestiste ja vürstide heraldikaga (S. Beletski)

  2. Uus Testament ja Piibel / Gustav Suits

    Index Scriptorium Estoniae

    Suits, Gustav, 1883-1956

    1999-01-01

    Varem ilmunud: Suits, Gustav. Eesti kirjanduslugu I. Lund : Eesti Kirjanike Kooperatiiv, 1953. Meie Issanda Jesusse Kristusse Uus Testament Ehk Jummala Ue Sädusse Sanna (1715). Eeltööd Vana Testamendi tõlkimiseks põhjaeesti keelde eesotsas Anton Thor Hellega. Ilmus täispiibel: Piibli Ramat, se on keik se Jummala Sanna...(1739)

  3. Käänuline ja kitsas Postitee taaselustab piirkonna põneva ajaloo / Signe Kalberg

    Index Scriptorium Estoniae

    Kalberg, Signe, 1959-

    2009-01-01

    Projekti "Postitee viidasüsteem ja puhkekohtade rajamine" raames 31. mail avatud viidastatud Postiteest (vana Tartu-Võru maantee teelõik), mille triibulised viidad, bussipeatuse pingid, objektitähised, mänguväljakud, infotarad jm. on kujundanud disainibüroo Laika, Belka & Strelka. Konkursi "Kaunis Eesti kodu 2009" Põlva maakondlik komisjon esitas Postitee viidaprojekti EV presidendile autasustamiseks

  4. Otel "priparkovalsja" / Ljudmila Pribõlskaja

    Index Scriptorium Estoniae

    Pribõlskaja, Ljudmila

    2005-01-01

    Riigikogu liikme Mark Soosaare sõnul on 80% küsitletud Pärnu elanikest vastu hotelli ehitamisele Pärnu vana pargi asemele. Kogutud on 5000 allkirja nimetatud pargiala kaitseks, M. Soosaar on pöördunud keskkonnaministri poole, kuid pole toetust leidnud

  5. Püha Siimeoni ja Naisprohvet Hanna kiriku restaureerimine. Tallinn, Ahtri 3 / Eva Mölder, Juhan Kilumets, Tõnu Parmakson...[jt.

    Index Scriptorium Estoniae

    2005-01-01

    3 sise- ja 3 välisvaadet; uuringud: J. Kilumets, E. Mölder; arhitektuurne projekt: T. Parmakson, I. Kannelmäe; konstruktiivne projekt: A. Danil, teostus: J. Kilumets (Rändmeister), T. Parmakson (Frantsiskus), E. Mölder (Vana Tallinn)

  6. Võru Gümnaasiumi restaureerimine ja juurdeehitus / Riho Arusoo

    Index Scriptorium Estoniae

    Arusoo, Riho

    2016-01-01

    Võru Gümnaasium asub kunagises Võru mõisa härrastemajas. Põhjalikud restaureerimistööd algasid hoones 2014. aasta juulis ja jõudsid valmis 2015. aasta augustis. Projekt hõlmas hoone kaht osa: üks täiesti uus ja teine uues kuues vana. Fassaadilahendusena võeti aluseks 1910. aasta projekt

  7. Vanalinna Hariduskolleegiumi Gümnaasiumi õppehoone (Tallinn, Vene 22) restaureerimine / Aleksandr Pantelejev, Helve Ilves, Villu Kadakas...[jt.

    Index Scriptorium Estoniae

    2009-01-01

    Ill.: situatsiooniplaan, lõige, 3 korruse plaani, värv. välisvaade, 7 sisevaadet; fotod: Kaido Haagen; OÜ Vana Tallinn; arhitektuuri sihtkapitali aastapreemia 2008 pälvisid: M. Hammer, T. Lõhmus, H. Ilves ja V. Kadakas

  8. Tallinna moenädala staarid

    Index Scriptorium Estoniae

    2008-01-01

    Tallinna moenädalal (Tallinn Fashion Week) 01.-08. nov. 2008. a. Rotermanni kvartali uues ja vanas jahulaos esitlevad oma rõivakollektsioone Kirill Safonov, Triinu Pungits, Ketlin Bachmann-Põldroos, Aldo Järvsoo, Ksenia Jedomskihh, Kalle Aasamäe. Moekunstnikud tutvustavad oma kollektsioonide ideed. Samal teemal lk. 6 sõnum "Tallinnal oma moenädal"

  9. Sisustusmess annab ideid / Kätlin Viksne

    Index Scriptorium Estoniae

    Viksne, Kätlin

    2011-01-01

    Sisustusmessist Interjöör 2011 ning aiandus- ja haljastusmessist Aiandus 2011 Eesti Näituste messikeskuses. Pikemalt projektidest "Vana hea"Hruštšovka"" (noore pere kodu ja modernse kodu kujundas Aet Piel), "Kirgas suvemaja" ja Eesti Kunstiakadeemia disainitudengite "Välknõuandla"

  10. Elo Viidingu kommentaarid Berk Vaheri kommentaarile / Elo Viiding

    Index Scriptorium Estoniae

    Viiding, Elo, 1974-

    2001-01-01

    Vt. ka Vaher, Berk. Mis teeb tuul müürile? : repriis Elo Viidingu sõnavõtule "Uued tuuled, vana vaev" (Looming 12/2000) // Sirp (2001) 12. jaan., lk. 6. Vastukaja: Vaher, Berk. Berk Vaheri kommentaarid Elo Viidingu kommentaaridele // Sirp (2001) 19. jaan., lk. 13

  11. Kallis reostuse avastamise seade ähvardab jääda tolmu koguma / Ulvar Käärt

    Index Scriptorium Estoniae

    Käärt, Ulvar, 1982-

    2009-01-01

    Eelarvekärbete tõttu puudub siseministeeriumil raha, et saada luba hakata pärast piirivalve lennusalga vana kopteri MI-8 kasutusaja täis saamist edaspidi õlireostuste avastamiseks muretsetud Lidar-seadet vedama olemasolevate Augusta Westland 139 kopterite või lennuki L-410 pardal

  12. Medieval socialist realism : Representations of Tallinn Old Town in Soviet Estonian Feature Films, 1969-1972 / Eva Näripea

    Index Scriptorium Estoniae

    Näripea, Eva

    2004-01-01

    Tallinna keskaegse vanalinna kujutamine Nõukogude Eesti mängufilmides : "Viimne reliikvia" (režii Grigori Kromanov, Tallinnfilm 1969), "Varastati Vana Toomas" (režii Semjon Shkolnikov, Tallinnfilm 1970), "Kolme katku vahel" (režii Virve Aruoja, Eesti Telefilm 1970), "Don Juan Tallinnas" (režii Arvo Kruusement, Tallinnfilm 1971), "Verekivi" (režii Madis Ojamaa, Tallinnfilm 1972)

  13. President Toomas Hendrik Ilves Will Speak At Columbia University

    Index Scriptorium Estoniae

    2010-01-01

    President Toomas Hendrik Ilves esineb 22. septembril 2010 Columbia Ülikoolis kõnega ""Vana ja uus" Euroopa pärast Lissaboni lepingu jõustumist". Töövisiit Ameerika Ühendriikidesse 20.-27.09.2010

  14. Maalide vahendusel sõjaeelsesse Narva

    Index Scriptorium Estoniae

    2005-01-01

    Näitus "Narva - enne ja pärast sõda" Narva kunstigaleriis. Sõjaeelset Narvat kujutavad Ardo Sivadi, Roman Vaheri ja Aleksander Normaku maalid ja graafika, sõjajärgset linna kujutavad Feliks Randeli ja Nikolai Tihhonovi joonistused 1944. aastast. Väljas on ka Carl Sarapi fotod 1939. a. ilmunud "Vana Narva" albumist. Karin Taidre kommentaar

  15. Posredstvom kartin - v dovojennuju Narvu

    Index Scriptorium Estoniae

    2005-01-01

    Näitus "Narva - enne ja pärast sõda" Narva kunstigaleriis. Sõjaeelset Narvat kujutavad Ardo Sivadi, Roman Vaheri ja Aleksander Normaku maalid ja graafika, sõjajärgset linna kujutavad Feliks Randeli ja Nikolai Tihhonovi joonistused 1944. aastast. Väljas on ka Carl Sarapi fotod 1939. a. ilmunud "Vana Narva" albumist. Karin Taidre kommentaar

  16. Improvizatsija v svetlõh tonah... / Irina Butjajeva

    Index Scriptorium Estoniae

    Butjajeva, Irina

    2008-01-01

    Kohtla-Järvel elava kunstniku Sergei Jatsenko maalinäitus "Improvisatsioon" Tallinnas Vana Viru hotellis. Näha on natüürmordid, maastikud ja naiste portreed. Kuraator on Olga Ljubaskina (galerii Sed Arte). Sergei Jatsenko on Vene Kunstnike Ühenduse liige. Kunstnikust

  17. Kaheksas maailmaime asus Sri Lanka saarel / Heiki Suurkask

    Index Scriptorium Estoniae

    Suurkask, Heiki, 1972-

    2000-01-01

    Sigiriya Lõvikalju on 370 meetri kõrgune kaljurahn keset dzunglit, mille tipus asunud lossist on säilinud kuninglik trooniiste vaatega kümnete kilomeetrite kaugusele ning 1500 aastat vana ujumisbassein siiani töökorras veevärgiga

  18. Tubakamüüja süüdistab riiki kahju tekitamises / Kadrin Karner ; kommenteerinud Hille Reinhold

    Index Scriptorium Estoniae

    Karner, Kadrin

    2010-01-01

    British American Tobacco Estonia ja Eesti Kaupmeeste Liit taotlesid riigilt vana maksumärgiga tubakatoodete müümistähtaja pikendamist kolmelt kuult kuuele. Kuna valitsus ettepanekut ei toetanud, leiab firma, et riik tekitas neile kahju 6,6 miljonit krooni

  19. Taevalikku rõõmu maisest sisustusest / Shanti Broeng

    Index Scriptorium Estoniae

    Broeng, Shanti

    2005-01-01

    Taani graafilise disaineri Rikke Larseni, tema abikaasa Larsi ja tütre Camille kodust 200-aastases maamajas Skibbys. Maja on sisustatud vana mööbli ja vanade esemetega. R. Larsen asutas koos emaga sisustuskaupluse Honning & Flora. 13 värv. ill

  20. Molecular characterization of vanA-containing Enterococcus from migratory birds: song thrush (Turdus philomelos

    Directory of Open Access Journals (Sweden)

    Nuno Silva

    2012-09-01

    Full Text Available Vancomycin-resistant enterococci (VRE were detected in two faecal samples (1.3% of song thrush in Portugal. vanA isolates showed high level vancomycin/teicoplanin resistance, as well as resistance to ciprofloxacin, quinupristin-dalfopristin and cloranfenicol. Thrush can be a reservoir of VRE and transmit these resistant bacteria to other animals including humans.

  1. Antibiotic resistance: methods and protocols

    National Research Council Canada - National Science Library

    Gillespie, S. H

    2001-01-01

    ... of the level and inducibility of resistance to vancomycin and teicoplanin. VanA type glycopeptide resistance is characterized by acquired inducible resistance to both vancomycin and teicoplanin and has been described for Enterococcus faecalis, Enterococcus faecium, Enterococcus gallinarum, Enterococcus casseliflavus, Enterococcus durans, Enteroco...

  2. Emergence of VanB phenotype-vanA genotype in vancomycin-resistant enterococci in Brazilian hospital

    Directory of Open Access Journals (Sweden)

    Zanella Rosemeire Cobo

    2006-01-01

    Full Text Available In Brazil, vancomycin-resistant enterococci (VRE have been reported as nosocomial pathogens since 1998. Recently, in a VRE surveillance in a hospital, we detected two Enterococcus faecalis isolates with vanA genotype and susceptible to teicoplanin. This is the first report of VanB phenotype-vanA genotype enterococci isolated from humans in Brazil.

  3. Meelelahutuslikkusele ei, teaduslikkusele jaa! / Peter van den Brink ; intervjueerinud Kadi Polli, Greta Koppel ; üles kirjutanud Greta Koppel

    Index Scriptorium Estoniae

    Brink, Peter van den

    2011-01-01

    Vestlus Kadrioru Kunstimuuseumis loengu pidanud Madalmaade kunsti spetsialisti Peter van den Brinkiga. Heast vana kunsti näitusest, uurimistöö mahust näituste ettevalmistamisel, monograafianäitustest, Michel Sittowi näituse vajalikkusest, näitusemaailma trendidest, Kadrioru Kunstimuuseumi maali "Õhtusöök Emmauses" autorsusest, kunstiteose tehnilisest uuringust jm.

  4. Aiamaja = Residential building in Aia street / Margit Mutso

    Index Scriptorium Estoniae

    Mutso, Margit, 1966-

    2010-01-01

    Tallinnas vanalinna piiril Vana-Viru 13/Aia 4 asuva elamu arhitektuursest lahendusest. Arhitektid: Villem Tomiste, Ott Kadarik, Mihkel Tüür (AB Kosmos). Ajaloolane Liivi Künnapu. Žürii liige Kalle Komissarovi žürii hinnangust kultuurkapitali aastapreemiale esitatud hoonele

  5. Soola päritolu / Andri Ksenofontov

    Index Scriptorium Estoniae

    Ksenofontov, Andri, 1962-

    2011-01-01

    Iiri kunstnike rühmituse Outrider Artists (Jackie Askew, Maeve Collins, Fiona Woods, Maria Kerin, Fiona O'Dwyer, Fergus Tighe) näitus "Salt" ("Sool") Tallinnas East Galeriis 20. märtsini 2011. Eesti kunstnike rühmitusest Vedelik, 6. märtsil peetud 9. märtsi tegevuskunsti festivalist Tallinnas galeriis Metropol (Vana-Kalamaja 46)

  6. Ida-igatsuse põhjapiir / Raivo Paltser

    Index Scriptorium Estoniae

    Paltser, Raivo

    2006-01-01

    Hiina traditsiooni järgija R. Paltseri nägemus Lääne inimese ihalusest Vana-Hiina kultuuri järele, kung-fust. Toodud mõningaid näiteid Soome ja Hiina mütoloogiat ühendavast fantaasiafilmist "Igavese armastuse sõdalane - Jade Warrior"

  7. Uus õppekava valmib 2010/2011. õppeaastaks / Kristi Helme

    Index Scriptorium Estoniae

    Helme, Kristi

    2008-01-01

    Riikliku õppekava nõukogu arutas uue õppekava lähteülesande eelnõu. Peeter Kreitzberg on arvamusel, et asutakse vana õppekava ümber tegema, mitte ei alustata päris algusest. Põhikooli õppekava kehtestatakse eraldi gümnaasiumi õppekavast. Õppekava nõukogust

  8. Suvelõpu performance Karepal / Hannes Varblane

    Index Scriptorium Estoniae

    Varblane, Hannes, 1949-

    2015-01-01

    15. augustil 2009 Karepal Richard Sagritsa majamuuseumis toimunud performance'ist "Vana riist", milles osalesid Hannes Varblane, Jüri Ojaver, Alex Plutzer, Ilja Sundelevitš, Jaan Paavle, Üllar Saaremäe, Leonhard Lapin, väljapanekutega lisandusid Valeri Vinogradov ja Jaan Toomik. Projektijuht Teet Veispak

  9. Soome-ugri rahvaste proosa jõudis eesti lugejate ette / Nikolai Kuznetsov, Vasli Nikolajev, Muš Nadii ; intervjueerinud Üve Maloverjan

    Index Scriptorium Estoniae

    Kuznetsov, Nikolai

    2013-01-01

    Autorid tutvustavad proosakogumikke: Vana maja monoloog : valik mari proosat : 26 lugu 23-lt autorilt aastatest 1927–2010. Tallinn, 2012 ; Seal kaugel-kaugel : valik komi proosat : 30 lugu 27-lt autorilt aastatest 1924-2010. Tallinn, 2011 ; Varsti on jälle kevad : valik udmurdi proosat : 39 lugu 34-lt autorilt aastatest 1890-2010. Tallinn, 2012

  10. Linnapea aktsiaid hoiab inkassofirma / Teet Roosaar

    Index Scriptorium Estoniae

    Roosaar, Teet, 1963-

    2003-01-01

    Pärnu linnapea Väino Hallikmägi müüs Via Ponti aktsiad inkassofirmale Pector, kes on varem kohtuvaidlustes Via Ponti esindanud. Linn ei ole rahul Via Ponti poolt Vana-Pärnu kaubasadama juurdepääsutee ehitamise tempoga

  11. Raamat / Matis Rodin

    Index Scriptorium Estoniae

    Rodin, Matis

    2012-01-01

    Raamatututvustus: Paulus, Karin. Tootedisain : asjad minu elus. Tallinn : Eesti Kunstiakadeemia, 2011; Vainküla, Tõnu, Pere, René. Vana mööbel korda oma kätega. [Tallinn] : Ajakirjade Kirjastus, 2012; Ruumipilt 2011 : Eesti parimad interjöörid ja disain / Eesti Sisearhitektide Liit ; toimetaja ja vastutav väljaandja Priit Põldme. Tallinn, 2012

  12. GenBank blastx search result: AK243477 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK243477 J100072I17 AF009672.1 AF009672 Acinetobacter sp. ADP1 VanR (vanR), vanilla...te demethylase (vanB), vanillate demethylase (vanA), and VanK (vanK) genes, complete cds; and unknown genes. BCT 1e-19 1 ...

  13. Kaduvusele kalduvad alternatiivid majas nr. 0 / Andreas Trossek

    Index Scriptorium Estoniae

    Trossek, Andreas, 1980-

    2002-01-01

    Prantsuse kunstnikekoloonia (Delphine Reist, Laurent Faulon, Sebastien Perroud) ja viiuldaja Mathieu Werchowsky Tallinnas valminud installatsiooninäitus "Neli laulu Siivile" vanas Kalinini tehasehoones 7.-12. juunini. Kunstnikud jätavad installatsiooni saatuse hooleks ja lähevad mujale

  14. Vanalinna Hariduskolleegiumi Gümnaasiumi õppehoone restaureerimine = Restoration of the academic building of the Old Town Educational College / Margit Mutso

    Index Scriptorium Estoniae

    Mutso, Margit, 1966-

    2009-01-01

    Tallinnas Vene 22 asuva hoone muinsuskaitse eritingimused koostas Aleksandr Pantelejev. Arhitekt Marie Hammer, sisearhitekt Tiiu Lõhmus (Vana Tallinn), arheoloog Villu Kadakas ja muinsuskaitselist järelevalvet teostanud Helve Ilves pälvisid restaureerimistöö eest arhitektuuri sihtkapitali aastapreemia 2008. Žürii hinnang

  15. Characterization of glycopeptide-resistant Enterococcus faecium (GRE) from broilers and pigs in Denmark: Genetic evidence that persistence of GRE in pig herds is associated with coselection by resistance to macrolides

    DEFF Research Database (Denmark)

    Aarestrup, Frank Møller

    2000-01-01

    Glycopeptide-resistant enterococci (GRE) from broilers and pigs were characterized to investigate the background for the persistence of GRE in pig herds. All porcine isolates belonged to closely related pulsed-field gel electrophoretic (PFGE) types, with the ermB and vanA genes located on the sam...

  16. Surveillance for vancomycin resistant enterococci in a tertiary ...

    African Journals Online (AJOL)

    Results: Thirteen of 319 patients surveyed were colonized with VRE; one with vanA E. faecium, two with vanB E. faecium, ten with E. gallinarum and one with E. casseliflavus. Univariate analysis for risk factors associated with VRE colonization was only significant for the ward of admission. Only one VRE isolate showed full ...

  17. Kunst on vaimu avardumise üks keskseid koldeid / Peeter Maria Laurits ; interv. Laura Kuusk

    Index Scriptorium Estoniae

    Laurits, Peeter, 1962-

    2006-01-01

    Peeter Laurits oma loomingust, näitustest "Voolav labürint" (koostöö Katzi ja Hollandi Pavlov Medialabiga) SEB Ühispanga galeriis, "Meistriteoste varjus" (koos Laurentsiusega) Kadrioru Kunstimuuseumis, isikunäitusest "Vana labürint" ArtDepoos, autorikaitsest jm.

  18. Pärtlipäeva mõtteid / Ants Piip

    Index Scriptorium Estoniae

    Piip, Ants, 1884-1942

    2007-01-01

    Vanas Testamendis kirjeldatavast "pärtliööst" Assüürias ja 1572. aasta sündmustest Prantsusmaal. Autorit köidavad ühelt poolt võimuahnuse ja teiselt poolt immateriaalsete kõrgemate ideede teenistuses oleva paindumatu meelekindluse probleemid.

  19. Pri svete vetra : Tjomnõi tunnel; Kogda-to na jarmarke; Daljoki, zabõtõi...; 'Hriplõi...'; Grustnaja pessenka; Bõla bõ metla...; Kannel; 'Pri svete vetra...'; Odnazhdõ; 'Kipariss...'; 'Na starom kostrisht'e...'; 'Vot spjashtshaja ja...'; 'Ser�

    Index Scriptorium Estoniae

    Vaarandi, Debora, 1916-2007

    1996-01-01

    Orig.: Tume tunnel; Vana laadalugu; Kauge unustatud; "See on..."; Kurb lauluke; Luuata; Kandlemängija; "Tuule valgel..."; Üks; "Küpress..."; "Magan tormis mis mujal..."; "Ööliblikaid halle ja maokaid..."; "naised on need..."; "Krestomaatsed luuletused..."; Sisevaade

  20. Tjomnõi tunnel : [luuletused] / Debora Vaarandi ; tlk. ja lühibiograafia: S. S. [Svetlan Semenenko

    Index Scriptorium Estoniae

    Vaarandi, Debora, 1916-2007

    1999-01-01

    Sisu: Tjomnõi tunnel ; Prostõje veshtshi ; Kogda-to na jarmarke ; Daljoki, zabõtõi... ; Grustnaja pessenka ; Bõla bõ metla ; Kannel ; "Pri svete vetra..." ; "Hrestomatiinõje virshi..." ; Vnutrenni vid. Orig.: Tume tunnel ; Lihtsad asjad ; Vana laadalugu ; Kauge unustatud ; Kurb lauluke ; Luuata ; Kandlemängija ; "Tuule valgel..." ; "Krestomaatilised luuletused..." ; Sisevaade

  1. Ohver vabadussambale : aimamatud müürid Harjumäel / Jüri Kuuskemaa

    Index Scriptorium Estoniae

    Kuuskemaa, Jüri, 1942-

    2008-01-01

    Vabadussõja võidusamba ehitamisega Tallinnasse lõhutakse vana kultuuri säilmeid. 1825. aastal koostatud Tallinna linnaplaanil ning Steingrübeli litograafial "Harju värav"on näha Ingeri bastion. Harjumäel Vabadussambale ette jäänud ning lammutatud müüristik ei olnud üllatus

  2. Inimlikumat keskkonda otsima / Hendrik Lindepuu

    Index Scriptorium Estoniae

    Lindepuu, Hendrik, 1958-

    1986-01-01

    Mark Soosaare "Lasnamäe" ("Tallinnfilm", 1985), Andres Söödi "Exegi monumentum" ("Tallinnfilm", 1985), Heini Drui "Elu vanas linnas" ("Tallinnfilm", 1985), Indrek Kangru "Alevimaastikud" ("Eesti Telefilm", 1985) ja Mati Põldre "Väike-Õismäe - rõngaslinn" ("Eesti Telefilm", 1985)

  3. Nooruslik kaupmeheemand kavatseb äri laiendada / Hille Tänavsuu

    Index Scriptorium Estoniae

    Tänavsuu, Hille, 1941-2014

    2006-01-01

    Vt. ka Postimees : na russkom jazõke 23. märts lk. 8. Tallinnas Vana-Kalamaja tänaval paikneva Alma Äri omanik Alma Sooäär avas oma esimese poe enam kui 15 aastat tagasi Nõmmel, praegu plaanib poeomanik majapidamistarvete äri laiendamist. Kaart: Alma Äri

  4. Kiht-kihilt tapeetidest / Kadri Kallaste

    Index Scriptorium Estoniae

    Kallaste, Kadri, 1983-

    2009-01-01

    Restauraator Kadri Kallaste ajalooliste tapeetide väärtusest, vanade tapeetide uurimise ja materjali kogumise vajalikkusest Eestis. Inglismaa tapeedifirmast Cole & Son, mis toodab endiselt tapeete käsitsi vanu meetodeid kasutades. Firma arhiivist, mille teenuseid saavad kasutada kõik, kellel soov taastada vana interjöör ajastukohast seinakatet kasutades

  5. Gut Alp im Kirchspiel St. Matthäi, Jerwen : Albu mõis, Järva-Madise kihelkond, Järvamaa / Konrad Heilmann

    Index Scriptorium Estoniae

    Heilmann, Konrad

    2006-01-01

    Albu mõis on vanim ordumõis Järvamaal, esmakordselt mainitud 1282. Mõisast, selle lähiümbrsusest ja omanikest läbi ajaloo. 1995-2000 restaureerimise käigus 200 aastat vana krohvikorra alt avastatud seinamaalingutest

  6. Filmikunsti kolm palet / Andres Maimik

    Index Scriptorium Estoniae

    Maimik, Andres, 1970-

    1999-01-01

    Tallinna filmifestival linastunud mängufilmid "Reekviem" : režissöör Alain Tanner : Šveits - Prantsusmaa - Portugal 1998, "Õnnelikult koos" : režissöör Wong Kar-Wai : Hongkong 1997 ja "Tuntud vana laul" : režissöör Alain Resnais : Prantsusmaa - Šveits - Suurbritannia 1997

  7. Tallinna Pisa torn on kaubahalli ees asuv viltuvajunud puumaja / Askur Alas

    Index Scriptorium Estoniae

    Alas, Askur, 1973-

    2005-01-01

    Ehitusekspert Heino Uuetalu hinnang Tallinna ühe vanima viltuvajunud puumaja seisukorrale. Heino Uuetalu on hoone rekonstrueerimisprojekti autor. Inseneri ja Aia tänava ristmikul asuv puumaja on ehitatud 18. saj. lõpus vana täidetud vallikraavi servale. Pisa tornist ja viltuvajunud vaatamisväärsustest Eestis

  8. Ralf Tamm - unistaja tehismaastikul / Ralf Tamm ; interv. Aino Ingrid Sepp

    Index Scriptorium Estoniae

    Tamm, Ralf

    2008-01-01

    Eramuile spetsialiseerunud arhitekt Ralf Tamm sellest, miks ta eelistab projekteerida eramuid, eramust visiooni kujunemisest, betoonist, hea arhitektuuri loomise eeldustest, eesti arhitektuuri lähitulevikust, tuleviku töövahenditest, enda tööstiilist jm. Abikaasa disainer Angela Orgusaar Ralf Tammest. Eramu "Vana laev" tellija Tarmo Ojakääru kommentaar. 13 ill

  9. Teatrihuviline kisub end täna õhtul lõhki / Raimu Hanson

    Index Scriptorium Estoniae

    Hanson, Raimu, 1957-

    2006-01-01

    26. jaan. on Tartus võimalik vaadata järgmisi lavastusi - Sagar Sagitta "Ajatud" Tampere majas, Vanemuise "Adolf" Püssirohukeldris, Võru Teatriateljee "Ilma luuminõ @ muu äri" Sadamateatris ja Emajõe Suveteatri "Frankenstein" vanas anatoomikumis

  10. Järva-Jaanis tulevad eriliselt helisevad jõulud / Bianca Micovitš

    Index Scriptorium Estoniae

    Mikovitš, Bianca

    2011-01-01

    Järva-Jaani kirik sai kaks uut Saksamaal valatud kirikukella. Kellad paigaldas Mäevali Orelitöökoda OÜ. Koos unikaalse vana kellaga (1478) läks vahetamisele väiksem kell, mis polnudki mõeldud tornis helistamiseks

  11. Eesti maalikunsti väljasuremist pole küll karta...

    Index Scriptorium Estoniae

    1999-01-01

    Eesti Maalikunstnike Liidu näitused. 17. juunil seinamaali konkurss Tallinna Kunstihoone hoovis. 18. juunist Kursi koolkonna uudislooming Raatuse galeriis, Eha Komissarovi kureeritud "Maal'99" Tallinna Kunstihoones, Ants Juske projekt "Uus ja vana narratsioon", mis ühendab maalilisuse, figuratiivsuse ja jutustuse eri kunstiliikides, Rotermanni soolalaos. Avamisel trio "Fragile" happening

  12. На Модной улице Baltika можно купить новинки эстонских брендов / Ксения Репсон

    Index Scriptorium Estoniae

    Репсон, Ксения

    2009-01-01

    Kontsern Baltika Grupp avas vanas tehasehoones Veerenni tänaval kaupluse Moetänav, kus müüakse firma kõigi kaubamärkide tooteid ja on avatud ka ReUse Republik kauplus. Tutvustatakse kaubamärkide Monton, Mosaic, Ivo Nikkolo ja Baltman uudistoodangut

  13. Tallinna linn premeeris 2006. aastal teostatud paremaid restaureerimistöid ja miljöösõbralikku uusarhitektuuri ajaloolises linnaruumis... / Riin Alatalu

    Index Scriptorium Estoniae

    Alatalu, Riin, 1968-

    2007-01-01

    Parim restaureeritud hoone vanalinna muinsuskaitsealal: Lai 43, omanik Kullaoru OÜ, restaureerimisettevõte Vana Tallinn. Paremini restaureeritud mälestis ajaloolises äärelinnas: Õie t. 21, omanikud Andres Nathan Kukk, Toomas Juhan Kukk, IB H. Uuetalu, Tondi kasarmute tellisfassaadide restaureerimine, Lindebergi Fassaadi OÜ. Parim uushoonestus ajaloolises keskkonnas: Koidu ja Loite t. vaheline kvartal, arendaja OÜ Hansakinnisvara, arhitektid Anu Tammemägi ja Erkki Ristoja. Projekti "Vana maja korda!" raames kõige paremini korrastatud majad: L. Koidula 2, KÜ Koidula 2, Kirde 10, omanik Ülle Mõistlik, värvilahenduse autorid Kristin-Mari Looveer ja Ene Odraks, Hiiu-Maleva 22, omanikud Jaak Margus Klaar ja Kristel Klaar, välisviimistluspassi koostajad K.-M. Looveer ja E. Odraks, Tähe t. 39/Lootuse pst. 107, omanik Meelika Maasik

  14. Increased conjugation frequencies in clinical Enterococcus faecium strains harbouring the enterococcal surface protein gene esp.

    Science.gov (United States)

    Lund, B; Billström, H; Edlund, C

    2006-06-01

    This study compared the in-vitro ability of Enterococcus faecium isolates of different origin to acquire vanA by conjugation in relation to the occurrence of the esp gene. In total, 29 clinical isolates (15/29 esp+), 30 normal intestinal microflora isolates (2/30 esp+) and one probiotic strain (esp-) were studied with a filter-mating assay. Conjugation events were confirmed by PCR and pulsed-field gel electrophoresis. Among the infection-derived isolates, the esp+ isolates had higher conjugation frequencies compared with esp- isolates (p < 0.001), with a median value of 6.4 x 10(-6) transconjugants/donor. The probiotic strain was shown to acquire vanA vancomycin resistance in in-vitro filter mating experiments.

  15. Marek Strandberg : "Estonija - ne Kuveit i ne dolzhna delat stavku na eksport svoihh energonossitelei" / Marek Strandberg ; interv. Aleksei Starkov

    Index Scriptorium Estoniae

    Strandberg, Marek, 1965-

    2007-01-01

    Riigikogu erakorralise, energeetikateemalise istungjärgu eel antud intervjuus ütleb EER-i üks juhte, et meie ühiskond elab 50 aastat vana energeetikaprogrammi järgi, praegu on vaja teha plaane järgmiseks 50 aastaks, et garanteerida endale energeetiline julgeolek. Põlevkivi põletamise asemel võiksime sellest teha sünteetilist gaasi

  16. Bacteriocin Production in Vancomycin-Resistant and Vancomycin-Susceptible Enterococcus Isolates of Different Origins

    OpenAIRE

    del Campo, Rosa; Tenorio, Carmen; Jiménez-Díaz, Rufino; Rubio, Carmen; Gómez-Lus, Rafael; Baquero, Fernando; Torres, Carmen

    2001-01-01

    Bacteriocin production was determined for 218 Enterococcus isolates (Enterococcus faecalis [93] and E. faecium [125]) obtained from different origins (human clinical samples [87], human fecal samples [78], sewage [28], and chicken samples [25]) and showing different vancomycin susceptibility patterns (vancomycin resistant, all of them vanA positive [56], and vancomycin susceptible [162]). All enterococcal isolates were randomly selected except for the vancomycin-resistant ones. A total of 33 ...

  17. Londoni aruanne / Jaan Elken

    Index Scriptorium Estoniae

    Elken, Jaan, 1954-

    2009-01-01

    Näitus "Uus kunst Lähis-Idast" Saatchi galeriis. Prantsuse installatsioonikunstniku Anette Messageri näitus "The Messengers" ("Sõnumitoojad") ja Mark Wallingeri kureeritud näitus "Russian linesman" ("Vene piirikohtunik") Haywardis Southbank Centre's. Tate triennaali näitus "Altermodern" ("Vana modernism"), kuraator Nicolas Bourriaud. Valik Pablo Picasso töödest "Challenging the past" ("Minevikku vaidlustades") The National Gallery's. Saksa maalikunstniku Gerhard Richteri näitus National Portrait Gallery's

  18. Säästva renoveerimise meistrikursused : SRIK-Tallinn (Väike-Patarei 3, Tallinn) / Toomas Tallo

    Index Scriptorium Estoniae

    Tallo, Toomas, 1940-

    2005-01-01

    Rein Tedrekulli koolitustsükkel (loengud Väike-Patarei 3, töötoad Liivalaia 19a): vanade uste restaureerimine - 1. III loeng, 5. III töötuba; vanade akende restaureerimine - 8. III loeng, 12. III töötuba; vana mööbli restaureerimine - 15. III loeng, 19. III töötuba. Siidimaali sissejuhatav kursus: 18. III Väike-Patarei 3 töötuba, mida juhendab kunstnik Evi Vares

  19. Säästva renoveerimise meistrikursused

    Index Scriptorium Estoniae

    2004-01-01

    Vanade uste korrastamine: 2. XI Tarmo Elvisto ja Jaan Sotteri loeng Väike-Patarei 3, 4. XI töötuba Liivalaia 19a Tallinnas. Vanade majade soklikorruste korrashoid ja renoveerimine (11. XI) ja vanade majade välisseinte, vahelagede, pööningute renoveerimine ja korrashoid (25. XI) - Heino Uuetalu loengud Väike-Patarei 3. Vana mööbli korrastamine ja restaureerimine: 27. XI töötuba Liivalaia 19a

  20. Sarviline must Gulliver heledate kääbikute keskel / Jüri Kuuskemaa

    Index Scriptorium Estoniae

    Kuuskemaa, Jüri, 1942-

    2009-01-01

    Arhitektuuribüroo Kosmos arhitektide Villem Tomiste, Ott Kadariku ja Mihkel Tüüri kavandatud valmimisjärgus äripindadega kortermaja Aia ja Vana-Viru t. nurgal Tallinna vanalinnas ei sulandu traditsioonilisse keskkonda. Eesti Muinsuskaitse Selts andis sümboolselt Karuteene medali isikutele ja institutsioonidele, kelle tõttu sai võimalikuks selle mastaabilt ja kujunduselt sobimatu uusehitise püstitamine vanalinna. Vastuseks Liina Tamme artiklile (Eesti Päevaleht, 2009, 16. juuli, lk. 7)

  1. 1. X kaitses Helsingi kunsti ja disainiülikoolis...

    Index Scriptorium Estoniae

    2005-01-01

    Ehtekunstnik Kärt Summatavet kaitses oma doktoritöö "Folk Tradition and Artistic Inspiration. A Woman's Life in Traditional Estonian Jewelry and Crafts as Told by Anne and Roosi", mis koosneb raamatust ning kolmest ehtenäitusest: "Lapsepõlv ja tüdrukuiga" (2000), "Noor naine. Emadus" (2001) ja "Vana tark naine" (2003). Oponendid olid Anzori Barkalaja ja Olli Tamminen

  2. Kangelane ja kaabakas: kuulsad kuningad muistse LähisIda ja Kreeka pärimustes / Mait Kõiv

    Index Scriptorium Estoniae

    Kõiv, Mait, 1961-

    2015-01-01

    Mesopotaamia, Pärsia ja Vana-Kreeka kolmest olulisest dünastiast. Akkadi kuningatest - Sargon'ist ja Naram-Sin'ist, Pärsia kuningatest Kyros'est ja Kambyses'est. Korintose türannidest Kypselos'est ja Periandros'est. Pärimuse tagamaadest: müütidest, propagandast ja reaalsusest. Dünastia rajaja kujutamisest kangelasena ja dünastia viimase esindaja demoniseerimisest

  3. Film Ita Everi juubeliks / Erika Klaats

    Index Scriptorium Estoniae

    Klaats, Erika, 1954-

    2006-01-01

    Näitleja Ita Everi 75-ndaks sünnipäevaks 6. aprillil peab ekraanile jõudma töös olev mängufilm "Vana daami visiit" : stsenaristid Roman Baskin, Ilmar Raag : režissöör Roman Baskin : peaosas Ita Ever, kaasa lööb üle 30 tuntud eesti näitleja : MTÜ Kell Kümme koostöös ETV ja Exitfilmiga, toetab ka Kultuurkapital

  4. Köök, mis polegi köök / Varje Talivee

    Index Scriptorium Estoniae

    Talivee, Varje

    2005-01-01

    Paar aastat tagasi valminud köök-söögituba Nõmmel vanas puumajas. Köögis on puupliit. Köögimööbli kavandas Kalle Pruuden, valmistas termotöödeldud puidust Hansanova. Skulptuurina mõjuva nagi kasvandasid K. Pruuden ja Uus-Meremaa mööblidisainer Benjamin Gilbert, valmistas Hansanova. 8 värv. ill

  5. Õllevabriku esiisa Saku mõis / Helju Koger

    Index Scriptorium Estoniae

    Koger, Helju, 1943-

    2004-01-01

    Saku mõisa ajaloost, omanikest, veneetsia akendega häärberist, maalitud ustest, lagedest, seintest. Mõisa praegune omanik on Saku vallavalitsus. Peahoone restaureerimisest, mille võttis ette 2002. a. hoone rentinud AS Saku Maja. Restaureeris OÜ Vana Tallinn, arhitekt Ive Punger, restauraator ja sisekujundaja Aet Maasik, kes kujundas ka pubi Sackenmeck. Fotod koridoride seintele aitas valida Ants Hein. Häärberis on puhke- ja seminarikeskus, allkorrusel Saku valla raamatukogu. 10 ill

  6. Koos kodus ja tööl / Milla Mägi

    Index Scriptorium Estoniae

    Mägi, Milla, 1949-

    2005-01-01

    Külaskäigust sisearhitektidest abielupaari Helle ja Taevo Gansi koju Nõmmel. Arhitekt August Volbergi projekti järgi 1937. a. valminud funktsionalistlik üherpereelamu on ümber ehitatud kahe pere elamuks. 1988. a. H. ja T. Gansi loodud perefirmast AS Stuudio Gadis, kus töötab ka üks lastest sisearhitekt Riia Oja. H. ja T. Gans oma perekonnast, tööst perefirmas, vana maja kujundamisest jm. 6 värv. ill

  7. Kui sind ei ole siin, siis...

    Index Scriptorium Estoniae

    Käesel, Tiina, 1943-

    2010-01-01

    Münchenis 62. rahvusvahelise käsitöömessi raames toimunud rahvusvahelisest konkurssnäitusest "Ehe" (Schmuck"), kuhu Eestist oli valitud Tanel Veenre tööga "Flora talv", konkurssnäitusest "Talendid" ("Talente"), Eesti ehtekunstnike rühmituse ÕhuLoss näitusest Müncheni Vana põhjakalmistu surnuaiavahi majas jt. ehtenäitustest

  8. Tarbekunst kaheksakümnendatel = Applied art in the eighties / Inge Teder

    Index Scriptorium Estoniae

    Teder, Inge, 1931-

    2010-01-01

    Eesti tarbekunsti arengut peegeldasid sel perioodil iga-aastased ülevaatenäitused, mitmed väljapanekud ja mahukad ruumikujundusliku teosed, seda eriti olümpiaregati uusehitistele. Osaliselt püüdis tarbekunst vabastada end materjali ja funktsiooni diktaadist ning astuda nn. vabade kunstide valdkonda, mis tekitas tuliseid diskussioone ja jäika vastuseisu. Üldpilt oli mitmetahuline - seal oli nii vana head tarbekunsti, kontseptuaalseid objekte ning kompositsioone, aga ka rahvusromantismi ja loodustemaatikat

  9. Pop nädal : Popuudised. POPtabel MTV.ru TOP 20

    Index Scriptorium Estoniae

    2002-01-01

    25. okt. esineb Eestis lansambel Saint Etienne, septembris tuleb müügile nende album "Finisterre". 7. sept. jõuab oma turnee käigus Tallinnasse norra grupp A-Ha. 19.-20. juulini kestva Hard Rock Laagri kohaks on Vana-Vigala. Restoranis L'Artiste (Hotell Olümpia) saab septembrini nautida Läti maalikunstniku Ritums Ivanovsi maale ansamblist Depeche Mode.

  10. Juhan Parts : näoga tuleviku poole / Juhan Parts ; interv. Märt Kivine

    Index Scriptorium Estoniae

    Parts, Juhan, 1966-

    2005-01-01

    Res Publica esimehe kohalt lahkuv Juhan Parts ütleb, et erakond on jäänud endaks - ausaks. Res Publica praeguse seisu üks põhjusi on tema arvates see, et erakond pole võtnud omaks vana poliitika lähenemisviise, ükski olemasolev erakond ei taha näha Res Publicat Eesti poliitikas. Lisa: Res Publica valib uue juhi

  11. Eesti firmad olid esmakordselt messil Denkmal 2000 / üles kirjut. Ingrid Eylandt-Kuure

    Index Scriptorium Estoniae

    2000-01-01

    Leipzigi messikeskuses 25.-28. X 2000. a. toimuva 4. muinsuskaitse ja linnauuenduse-alase messil oli ühisekspositsiooniga (põhiteemaks Tallinna tüüpiline elamu XV sajandist 1930. aastateni, boksi kujundaja Krista Veermets) väljas Eesti esindus: Tallinna Kultuuriväärtuste Amet, OÜ Arhitektuuribüroo Adrikorn & Rets, OÜ Vana Tallinn, AS KAR-Grupp. Messimuljeid

  12. Igrõ s klassikoi : vesseltshaki Turgenev i Dostojevski i bezzhalostnõi Tshehhov / Boris Tuch

    Index Scriptorium Estoniae

    Tuch, Boris, 1946-

    2006-01-01

    Aljona Aljohhina lavastus "Palat nr 6" Anton Tshehhovi jutustuse järgi Vene Teatris. Mark Rozovski lavastus "Krokodill" Vana Baskini Teatris. Teatriteksti kirjutas lavastaja Fjodor Dostojevski samanimelise pamfleti aineil. Ivan Turgenevi "Kuu aega maal" Eesti Draamateatris, lavastaja Mihhail Mokejev Moskvast. Mattias Anderssoni "Kuritöö, kodutööd, karistus, raha, pensionäri tapmine" Fjodor Dostojevski ainetel Teises Teatris, lavastaja Erki Aule. Artikkel järgneb nr. 47, 24. nov. 2006, lk. 38

  13. Vihterpalu mõisa restaureerimine / Silja Konsa

    Index Scriptorium Estoniae

    Konsa, Silja

    2006-01-01

    Lühidalt mõisa ajaloost. 2001. a. omandas mõisa soomlasest ärimees Timo Lemberg. Peahoone remondi-restaureerimistöödest aastail 2001-2005. Uuringud, projekt, muinsuskaitseline järelevalve: OÜ Vana Tallinn. Teostus: AS Suntee, krohvitööd Lindebergi Fassaadi OÜ, stuki ja maalingute restaureerimine AS KAR Grupp. Mõisas on puhke- ja konverentsikeskus. Välisvaade, 2 sisevaadet

  14. Buddolog Otton Ottonovitsh Rozenberg i Estonia / Mart Läänemets

    Index Scriptorium Estoniae

    Läänemets, Mart

    2006-01-01

    Vana-Liivimaal sündinud ülemaailmse tuntusega orientalist Otto Rosenberg arendas edasi Vassili Vassiljevi ideed "graafilisest alfabeedist" - hieroglüüfide järjestatusest nende graafiliste elementide järgi, temast sai Peterburi ülikooli esimene doktor jaapani keele ja kirjanduse alal, ta suri Tallinnas 26. novembril 1919 ja maeti Kopli kalmistule. Lisatud dokumendid, mis tõestavad tema surma 1919. aasta sügisel Tallinnas

  15. Sotsiaalselt pime ja poliitiliselt impotentne teater / Alvar Loog

    Index Scriptorium Estoniae

    Loog, Alvar

    2006-01-01

    Poliitilisest ja sotsiaalsest teatrist Eestis 2005. aasta lavastuste põhjal : Pip Upponi "Adolf" Tartu Püssirohukeldris, lavastaja Ervin Õunapuu; Merle Karusoo "Misjonärid" Rakvere Teatris; Alistair Beatoni "Õnnetunne" Ugalas, lavastaja Jaak Allik; Toomas Kalli " Viimane sent" Vana Baskini Teatris, lavastaja Eino Baskin; George Orwelli "Loomade farm" Tallinna Linnateatris, lavastaja Mart Koldits; Kai Henseli "Klammi sõda" VAT Teatris, lavastaja Aare Toikka

  16. Pesa moodi kodu / Liina Jänes

    Index Scriptorium Estoniae

    Jänes, Liina, 1977-

    2004-01-01

    Tallinnas kivitrepikojaga kortermajas asuva noore tekstiilikunstniku kööktoa (16 mø) kujundusest ja sisustusest. Toas on vana mööbel, klaasiga eraldatud duširuum, puupliit, ahi. Köögiosa riiulid ja laud on ise kavandatud. Duširuumi, kööginurga ja välisukse esine põrand on plaaditud, magamis- ja elupoolele jääb laudpõrand. 10 ill

  17. Professor Raimo Pullat võistleb ajalooteaduse raskekaalus / Rein Veidemann

    Index Scriptorium Estoniae

    Veidemann, Rein, 1946-

    2004-01-01

    Rets. rmt.: Pullat, Raimo. Lootuste linn Peterburi ja eesti haritlaskonna kujunemine kuni 1917. Tallinn, 2004 ; Briefwechsel zwischen Jan Bauduoin de Courtenay und Jooseppi J. Mikkola aus den jahren 1898-1926. Krakow, 2004 ; Die Nachlassverzeichnisse der deutschen Kaufleute in Tallinn 1777-1800. Band 3 = Tallinna saksa kaupmeeste varandusinventarid 1777-1800. 3. köide. Tallinn, 2004 ; Vana Tallinn XV (XIX). Tallinn, 2004

  18. Arhitektuuriuudised / Karen Jagodin

    Index Scriptorium Estoniae

    Jagodin, Karen, 1982-

    2007-01-01

    Radikaalne kortermaja Itaalias Bolzanos, kus terrassid ja korteri vahelduvad fassaadil ja lähevad sujuvalt üksteiseks üle. Pouya Khazaeli Parsa projekteeritud villa Teherani lähedal, mis valminud Le Corbusier' loomingu eeskujul. Bitumi ja Pekka Manneri ülimoodne avalik tualett Suomenlinnas. Wolfgang Feyferliki ja Susi Fritzeri projekteeritud eramu, mis sobitub edukalt tammede vahele. Mätaskatusega pargipaviljon Lääne-Holsteinis. Šveitsi Alpidesse projekteeritud elamiskompleks, kus leitud vanaeuroopalik tasakaal vana ja uue vahel

  19. Lohu mõis / Christian Gottlieb Welté

    Index Scriptorium Estoniae

    Welté, Christian Gottlieb, 1748/49-1792

    1996-01-01

    lk. 186 (härrastemaja, foto 20. saj. algusest), lk. 187 (fragment 1915. a. plaanist), lk. 188-189 (vana härrastemaja, hilisem valitsejamaja), värv., lk. 190-191 (illusionistlikud seinamaalingud saalis, 1791, G. Chr. Welté), värv., lk. 192-193 (saali Don Quijote teemalised pilttapeedid, 19. saj. I veerand), värv.

  20. Lipophilic teicoplanin pseudoaglycon derivatives are active against vancomycin- and teicoplanin-resistant enterococci.

    Science.gov (United States)

    Szűcs, Zsolt; Bereczki, Ilona; Csávás, Magdolna; Rőth, Erzsébet; Borbás, Anikó; Batta, Gyula; Ostorházi, Eszter; Szatmári, Réka; Herczegh, Pál

    2017-05-01

    A selection of nine derivatives of teicoplanin pseudoaglycon were tested in vitro against clinical vancomycin-resistant Enterococcus strains possessing vanA, vanB or both genes. The bacteria were characterized by PCR for the identification of their resistance genes. The tested compounds contain lipoic acid, different carbohydrates and aryl groups as lipophilic moieties. About one-third of the teicoplanin-resistant strains were shown to be susceptible to one or more of the glycopeptide derivatives.

  1. Uuskasutus ja kompromissid / Triin Talk

    Index Scriptorium Estoniae

    Talk, Triin

    2015-01-01

    Ajalooliste hoonete kasutuses hoidmisest, neile uue funktsiooni andmisest. Vana-Võidu mõisa karjakastellist, mis kohandati Viljandi Kutseõppekeskuse õppehooneks. OÜ Paalakalda korrastatud Pajusi mõisa kuivatist. Väimela mõisa lauta rajatud Võrumaa Kutsehariduskeskuse puidutöötlemise ja mööblitootmise kompetentsikeskusest. Mooste mõisa meiereisse rajatud tervisekeskusest. Olustvere mõisakompleksi meiereisse rajatud Olustvere Teenindus- ja Maamajanduskooli meemajast

  2. Dostojevski aeg / Boris Tuch ; tõlk. Madis Kolk

    Index Scriptorium Estoniae

    Tuch, Boris, 1946-

    2007-01-01

    Dostojevski kangelased Eesti teatrilaval: "Onu unenägu" Vene Teatris (lavastaja Mihhail Tshumatshenko), Matthias Andersoni "Kuritöö, kodutööd, karistus, raha, pensionäri tapmine" Drugoi Teatr (lavastajad Erki Aule ja Artjom Garejev), Eesti - Läti - Leedu vene teatrite koostööprojekt "Russki smehh" (lavastaja Roman Kozak), "Sortsid" Eesti Draamateatris (lavastaja Hendrik Toompere jun.), "Krokodill" Vana Baskini Teatris (lavastaja Mark Rozovski)

  3. Ago Soots tuli Võrru "Peole" / Ago Soots ; intervjueerinud Kaile Kabun

    Index Scriptorium Estoniae

    Soots, Ago

    2009-01-01

    Intervjuu Võrumaalt pärit VAT Teatri näitleja ja lavastaja Ago Sootsiga, kes mängib peaosa Võru linnateatri suvelavastuses "Pidu". Slawomir Mrožeki näidendi lavastab Uku Uusberg, esietendus 17. juunil Võru vanas viinavabrikus. Ago Soots oma kooliajast, tööst VAT Teatris, huvist muusika ja spordi vastu, näitlejaametist, lavastusest

  4. Severstaltrans muutub Eesti kütuse- ja transiidiäri valitsejaks / Peeter Raidla

    Index Scriptorium Estoniae

    Raidla, Peeter, 1955-

    2004-01-01

    Ilmunud ka: Delovõje Vedomosti 10. nov. lk. 6. Venemaa transiidihiid Severstaltrans omandas AS-i E.O.S. enamusosaluse. Diagramm: E.O.S.-i majandustulemused. Lisad: Vana E.O.S. muutus uueks E.O.S.-iks; Eelmine omanikuvahetus jättis E.O.S.-i omaniku samaks; Severstal on tõeline Venemaa tööstushiid

  5. Püha Siimeoni ja Naisprohvet Hanna kiriku restaureerimine : Ahtri 5, Tallinn = Restoration of the Church of Righteous Symeon and Prophetess Ann : Ahtri 5, Tallinn / Epp Lankots

    Index Scriptorium Estoniae

    Lankots, Epp, 1976-

    2005-01-01

    18. sajandil ehitatud, 2001. a. Eesti Apostlik-Õigeusu Kirikule tagastatud kirikuhoone restaureerimine. Ees on interjööride konserveerimistööd. Uuringud: Juhan Kilumets, Eva Mölder. Arhitektuurne projekt: Tõnu Parmakson, Ilmar Kannelmäe. Konstruktiivne projekt: Aleksander Danil. Teostus: J. Kilumets (Rändmeister), T. Parmakson (Frantsiskus), E. Mölder (Vana Tallinn). Ill.: 3 värv. välis- ja 3 sisevaadet

  6. Fitness costs of various mobile genetic elements in Enterococcus faecium and Enterococcus faecalis

    Science.gov (United States)

    Starikova, Irina; Al-Haroni, Mohammed; Werner, Guido; Roberts, Adam P.; Sørum, Vidar; Nielsen, Kaare M.; Johnsen, Pål J.

    2013-01-01

    Objectives To determine the fitness effects of various mobile genetic elements (MGEs) in Enterococcus faecium and Enterococcus faecalis when newly acquired. We also tested the hypothesis that the biological cost of vancomycin resistance plasmids could be mitigated during continuous growth in the laboratory. Methods Different MGEs, including two conjugative transposons (CTns) of the Tn916 family (18 and 33 kb), a pathogenicity island (PAI) of 200 kb and vancomycin-resistance (vanA) plasmids (80–200 kb) of various origins and classes, were transferred into common ancestral E. faecium and E. faecalis strains by conjugation assays and experimentally evolved (vanA plasmids only). Transconjugants were characterized by PFGE, S1 nuclease assays and Southern blotting hybridization analyses. Single specific primer PCR was performed to determine the target sites for the insertion of the CTns. The fitness costs of various MGEs in E. faecium and E. faecalis were estimated in head-to-head competition experiments, and evolved populations were generated in serial transfer assays. Results The biological cost of a newly acquired PAI and two CTns were both host- and insertion-locus-dependent. Newly acquired vanA plasmids may severely reduce host fitness (25%–27%), but these costs were rapidly mitigated after only 400 generations of continuous growth in the absence of antibiotic selection. Conclusions Newly acquired MGEs may impose an immediate biological cost in E. faecium. However, as demonstrated for vanA plasmids, the initial costs of MGE carriage may be mitigated during growth and beneficial plasmid–host association can rapidly emerge. PMID:23833178

  7. Suvi 2007 tõotab tulla rokisõpradele magus / Kadri Ratt

    Index Scriptorium Estoniae

    Ratt, Kadri

    2007-01-01

    5. juulil Tallinnas A.Le Coq Arenal esinevast ameerika bändist Aerosmith, festivalidest "Rabarock" 15. ja 16. juunil Järvakandis ning "Hard Rock Laager" 29. ja 30. juunil Vana-Vigalas, ameerika bändi Type O Negative kontserdist 10. juunil Tallinnas Rock Cafés, brasiilia bändi Sepultura kontserdist 22. juulil Tallinnas Rock Cafés

  8. Suur üllataja Albu mõis / Helju Koger

    Index Scriptorium Estoniae

    Koger, Helju, 1943-

    2004-01-01

    Restaureeritud Albu mõisakoolist, kivisillast ja toiduaidast, pargi rekonstrueerimisest jm. Arhitekt Jaan Jõgi. Sisekujundaja Nele Rohtla OÜst Mõisaprojekt. Maalingud restaureeris OÜ Vana Tallinn eesotsas restauraator Aet Maasikuga, kes tegi ka seinte marmoreeringud. Toiduaida mööbli ja mõisamotiividega serviisi kavandas Tõnu Reeveer. Mõisa omanikest. 2004. a. oli häärberis endiste omanike järeltulija, Kanadas elava Gerhard von Harpe maalide näitus. 12 ill

  9. "Olen viimane Napoleoni sõdur..." : [luuletused] / Charles Simic ; inglise keelest tõlkinud Andres Ehin

    Index Scriptorium Estoniae

    Simic, Charles

    2007-01-01

    Sisu: "Olen viimane Napoleoni sõdur..." ; "Luuletus koosviibimisest jahedal sügisõhtul..." ; "Kas vene inimsööjad on hullemad kui inglise omad?..." ; "Vana tunkedes talumees on enda laudalae aampalgi külge üles poonud..." ; "Ta oli segi ajanud tegelaskujud selles pikas romaanis..." / luulekogust "Hirmuäratavad mänguasjad" ; Mu hääletu kaaskond ; Õlenukk ; Tapamaja kärbsed ; Amelia pärast

  10. Tavatu kodu : ruum ruumis

    Index Scriptorium Estoniae

    2004-01-01

    Arhitekt Paula Butterfieldi ja kujundusgraafik Gary Macphersoni loft-stiilis kodu vanas renoveeritud telliskivihoones Manchesteris. Vaheseinteta korter moodustab avatud ruumi, mille sisse on ehitatud boks. Boksi peal on magamislavats külalistele, sees magamisruum, vannituba ja panipaik, seina taga köök, põranda all torustik. Pesemisruumi konstruktsioonid on valatud klaasplastist ja kaetud veekindla SIKA-krohviga. Tualettruumi seinu katab roostevaba teras. 1 välis- ja 12 sisevaadet

  11. Uued mälestised aastal 2015 ja kadunud mälestised 2013-2015 / Triin Talk

    Index Scriptorium Estoniae

    Talk, Triin

    2016-01-01

    Kolmest uuest ehitusmälestisest: Rapla KEK'i haldushoonest, Kulla leerimajast ja kultuuriseltsi Koit majast. Kultuurimälestiste hulgast on välja arvatud: Vana-Saaluse park, Roti vesiveski küün, Sangaste mõisa rehe 2 varemed, Maidla mõisa teenijatemaja ja valitsejamaja, Porkuni mõisa aednikumaja ja Sindis Kooli 8 asuv ouidust tööliselamu

  12. Linnamüür säilib

    Index Scriptorium Estoniae

    2007-01-01

    Ehitusfirma Restor plaan ehitada Tallinna linnamüüri äärde korterelamud jäi viimasel hetkel katki, sest Tallinna linnal õnnestus vahetada osa Suurtüki 4c asuvast kinnistust Vana-Viru t. 3 kinnistu vastu. Restor ostis Suurtüki t. krundi ja ehitised riigilt 1997. a. ja sai linnalt loa need korterelamuteks ümber ehitada

  13. Maja merega rahujalal / Jana Rand

    Index Scriptorium Estoniae

    Rand, Jana, 1963-

    2005-01-01

    Suurema osa elust Rootsis elanud Gerti, kelle vanemad lahkusid Eestist sõja eel, ja Raija Hirschi Eestis vana taluaseme kohal mere ääres asuvast, Anu Kotli projekteeritud majast, mis on oma proportsioonid saanud talurahvaarhitektuurist. Hoone on puitlaudisega, läänepoolne ots on suures osas klaasist. Sisekujundaja Vello Liiv. Kasepuust köögi- ja vannitoamööbel on valmistatud eritellimuse järgi Saaremaa mööblifirmas Kalla. 10 ill

  14. Ülemiste järve esitlev elegantne hotell : Lennujaama tee 2, Tallinn = An Elegant Hotel that Presents Lake Ülemiste : Lennujaama tee 2, Tallinn / Urmas Oja

    Index Scriptorium Estoniae

    Oja, Urmas, 1981-2012

    2004-01-01

    Aerofloti hotelli kohale ja osalt vana maja konstruktsioonidele ehitatud Ülemiste hotellist. Hotelli ees on parkla ja purskkaevuga väljak. Projekteerija: EA Reng, projektijuht Taavi Turvas. Arhitekt Martin Aunin. Sisekujundus: Meelis Press, Hillar Mänd (M. Pressi Arhitektuuribüroo). Konstruktsioonid: Inseneribüroo Pike. Ehitaja: FKSM. I, II ja III korruse plaan, 3 välis- ja 5 sisevaadet

  15. Monika Salu imemaja püsib ka külma ilmaga soe / Bianca Mikovitš

    Index Scriptorium Estoniae

    Mikovitš, Bianca

    2011-01-01

    Monika Salu juhitav OÜ Inotex Grupp taastab Vilsandi tuletorni vahimaja näidismajaks. Eesmärk on kõige optimaalsem ja energiasäästlikum lahendus, mida on võimalik teha, säilitades vana ja tagades muinsuskaitseväärtuse säilimise. Töö käib samuti merepäästejaama restaureerimisega

  16. Antibiotic resistance and molecular characterization of probiotic and clinical Lactobacillus strains in relation to safety aspects of probiotics.

    Science.gov (United States)

    Klein, Günter

    2011-02-01

    The evaluation of the safety of probiotic strains includes the exclusion of antibiotic resistance of clinical importance. Ninety-two strains from the genus Lactobacillus isolated from probiotics, food, and clinical sources were included in the investigation. Species tested were the L. acidophilus group, L. casei group, L. reuteri/fermentum group, and L. sakei/curvatus group. Cell and colony morphology, fermentation patterns, and growth characteristics as well as soluble whole cell proteins were analyzed. Antibiotic resistance against clinically important agents was determined by broth dilution tests. The vanA and tet genes were confirmed. Resistances occurred mainly against gentamicin, ciprofloxacin, clindamycin, sulfonamides, and, in some cases, glycopeptides. The natural glycopeptide resistance within the L. casei group and L. reuteri appears to be not of clinical relevance, as there was no vanA gene present. Therefore, the transfer of this resistance is very unlikely. Tet-(A), -(B), -(C), -(M), or -(O) gene could not be detected. The protein fingerprinting within the L. casei group proved that L. rhamnosus strains of clinical origin clustered together with probiotic strains. For safety evaluations resistance patterns of a broad range of strains are a useful criterion together with the exclusion of known resistance genes (like the vanA gene) and can be used for decision making on the safety of probiotics, both by authorization bodies and manufacturers.

  17. [Epidemiological analysis for enterococci isolated in Aichi prefecture].

    Science.gov (United States)

    Sakanashi, Daisuke; Yamagishi, Yuka; Suzuki, Takayoshi; Ohno, Tomoko; Yamada, Atsuko; Suematsu, Hiroyuki; Mikamo, Hiroshige

    2014-04-01

    We investigated vancomycin-resistant genes for clinical isolates of 353 vancomycin-resistant enterococci in the Aichi Medical University hospital and 120 vancomycin-resistant enterococci from the 8 facilities in Aichi prefecture between April 2008 and January 2013. We detected 8, 105, 21 and 4 strains of enterococci with vanA, vanB, vanC1 and vanC2/C3, respectively. Among enterococci with vancomycin-resistant genes, we detected 4, 3 and 2 enterococci of vancomycin MIC level 4 microg/mL with vanB, vanC1 and vanC2/C3, respectively. According to molecular analysis using repetitive-sequence-based PCR (rep-PCR) for enterococci with vanA or vanB genes, although there have been no similarity for Enterococcus faecium with vanA and Enterococcus faecalis with vanB, high similarity was shown among E. faecium with vanB, which might be nosocomial spread in each hospital. These results showed that molecular analysis for vancomycin-resistant genes would be useful for the management of healthcare-associated infections.

  18. vanA-targeted oligonucleotide DNA probe designed to monitor vancomycin- and teicoplanin-resistant bacteria in surface waters.

    Science.gov (United States)

    Nakipoglu, Mustafa; Yilmaz, Fadime; Icgen, Bulent

    2016-10-01

    The glycopeptide antibiotics teicoplanin and vancomycin are common to treat severe Gram-positive bacterial infections. The gene vanA confers high-level resistance to these antibiotics, and these phenomena have been shown to be transferable. Release of vancomycin- and teicoplanin-resistant bacteria to surface waters is, therefore, of particular concern since they might proliferate and spread in different environments. Monitoring of the fate of vanA gene in these waters provides information on the exposure and potential threats of those bacteria for the environment and public health. Therefore, this study aimed at preparing a 25-mer-oligonucleotide DNA probe based on the 909 bp BamHI-ClaI fragment from Enterococcus faecium plasmids pVEF1 and pVEF2 through the use of Vector NTI Express Software. The newly designed vanA probe displayed highly specific hybridization with vanA-positive Enterococcus faecalis tested at 46 °C, 55 % formamide, and 0.020 M NaCl stringency conditions. In situ fluorescein hybridizations under the same stringency conditions were also used to monitor river water samples by using fluorescein microscopy. The results showed that the vanA-targeted oligonucleotide DNA probe prepared was not only highly specific but also quantitative tool for monitoring vancomycin- and teicoplanin-resistant bacteria in surface waters.

  19. Epidemiologic evaluation of Vancomycin Resistant genes in Enterococcus spp. isolated from clinical samples

    Directory of Open Access Journals (Sweden)

    Omid Teymournejad

    2011-09-01

    Full Text Available Background & Objectives: Isolation of vancomycin resistant Enterococcus from clinical samples is very important. The aim of this study was evaluation of phenotype and genotype of van genes in vancomycine resistant Enterococcus. Materials and Methods: 411 Enterococcus isolates were collected from selected Tehran’s hospitals between March 2004 and December 2007. The enterococcal isolates were identified by biochemical confirmation tests. Resistance of each isolate to vancomycin determined by disk diffusion and agar dilution test. The presence of the vanA, B, C, D, E resistance gene was assessed by PCR. Results: 185(45% and 23(5.6% with disc-diffusion method and agar-dilution method were resistant to vancomucin (VRE and all of VREs were Enterococcus faecium. 12 (52.2%, 7(30.4% of the VRE isolates had vanA, vanB and 3(13% had both of vanA and vanB gene. Conclusion: Most important mechanism for high level resistance to vancomycin is presence of van genes and these genes can transfer between Enterococci. Significance of investigation in molecular level of resistance to vancomycin was due to relation between phenotypic resistant and presence of van genes.

  20. Faster and economical screening for vancomycin-resistant enterococci by sequential use of chromogenic agar and real-time polymerase chain reaction.

    Science.gov (United States)

    Tan, Thean Yen; Jiang, Boran; Ng, Lily Siew Yong

    2017-08-01

    Screening for vancomycin-resistant enterococci (VRE) by culture takes days to generate results, while polymerase chain reaction (PCR) testing directly from clinical specimens lacks specificity. The aims of this study were to develop a real-time PCR to detect and identify Enterococcus faecium, Enterococcus faecalis, and vanA and vanB genes, and to evaluate the impact of this PCR on test-reporting times when performing it directly from suspect VRE isolates present on screening chromogenic media. The tetraplex PCR primers were designed to amplify E. faecium, E. faecalis, and vanA and vanB genes, with melt-curve analysis of PCR products. Following analytical and clinical validation of the molecular assay, PCR testing was performed for target colonies present on VRE chromogenic media. PCR results were evaluated against conventional phenotypic identification and susceptibility testing, with the time to result being monitored for both modalities. A total of 519 colonies from clinical specimens were tested concurrently by real-time PCR and phenotypic methods. In all, 223 isolates were identified with phenotypic vancomycin resistance (vanA, n = 108; vanB, n = 105; non-vanA/vanB = 10), with complete agreement between PCR and phenotypic testing for vancomycin-resistant E. faecium and E. faecalis. The majority (88.6%) of PCR results were reported, on average, 24.8 hours earlier than those of phenotypic testing, with 68% reduction in total costs. The use of culture on selective media, followed by direct colony PCR confirmation allows faster and economical VRE screening. Copyright © 2015. Published by Elsevier B.V.

  1. Molecular characteristics of vancomycin-resistant Enterococcus faecium from a tertiary care hospital in Chengdu, China: molecular characteristics of VRE in China.

    Science.gov (United States)

    Kang, M; Xie, Y; He, C; Chen, Z X; Guo, L; Yang, Q; Liu, J Y; Du, Y; Ou, Q S; Wang, L L

    2014-06-01

    The aim of this study was to characterize vancomycin-resistant Enterococcus faecium (VREfm) isolates phenotypically and molecularly, and investigate associations between the virulence factors enterococcal surface protein (esp), hyaluronidase (hyl), and collagen adhesin (acm) and colonization/infection. A total of 126 E. faecium [66 VREfm and 60 vancomycin-susceptible (VSEfm)] were collected in West China Hospital. Nine E. faecium isolates (7 VREfm and 2 VSEfm) were selected at random for comparative study in a large region from China. Minimum inhibitory concentrations (MICs) were measured by Etest and agar dilution, vancomycin resistance genes (vanA, vanB, and vanC) and virulence genes (esp, acm, and hyl) were detected by polymerase chain reaction (PCR). Thirty-four VREfm underwent repetitive sequence-based PCR (rep-PCR) and multi-locus sequence typing (MLST). One linezolid-resistant isolate (MIC = 8 μg/ml) was found; none were tigecycline resistant. All 73 VREfm (28 infective strains and 45 intestinal colonizers) had the vanA gene and VanA phenotype. Positivity for esp, hyl, and acm in VREfm was 79.5, 46.6, and 86.3%, respectively, which was higher than in VSEfm (54.8, 27.4, and 56.5%, respectively). Among VSEfm, positivity for acm in isolates from pleural or cerebrospinal fluid (84.6%) was higher than that from blood (32.4%). There were 11 rep-PCR types (similarity >95%) and MLST revealed nine sequence types (STs) among the selected isolates. Most VREfm and all VSEfm belonged to clonal complex 17. A new ST was found, with allele sequence (15, 1, 38, 1, 1, 1, 1). In China, most VREfm seem to belong to the classical nosocomial CC17 clone, and many of them have acquired virulence genes, further strengthening a hospital-adapted type.

  2. Molecular characterization of resistance, virulence and clonality in vancomycin-resistant Enterococcus faecium and Enterococcus faecalis: A hospital-based study in Beijing, China.

    Science.gov (United States)

    Yang, Jing-xian; Li, Tong; Ning, Yong-zhong; Shao, Dong-hua; Liu, Jing; Wang, Shu-qin; Liang, Guo-wei

    2015-07-01

    The incidence of vancomycin-resistant enterococcus (VRE) in China is increasing, the molecular epidemiology of VRE in China is only partly known. This study was conducted to assess the molecular characterization of resistance, virulence and clonality of 69 vancomycin-resistant Enterococcus faecium (VREfm) and seven vancomycin-resistant Enterococcus faecalis (VREfs) isolates obtained from a Chinese hospital between July 2011 and July 2013. The glycopeptide resistance genes (VanA and VanB) were screened by multiplex PCR. The presence of five putative virulence genes (esp, gelE, asa1, hyl and cylA) were evaluated by another multiplex PCR. Multilocus sequence typing (MLST) scheme was used to assess the clonality. All 76 VRE isolates exhibited VanA phenotype and harbored VanA gene. Esp was the only gene detected both in VREfm and VREfs strains, accounting for 89.9% and 42.9%, respectively. The hyl gene was merely positive in 27.5% of VREfm strains. MLST analysis demonstrated three STs (ST6, ST4 and ST470) in VREfs and twelve STs (ST78, ST571, ST17, ST564, ST389, ST18, ST547, ST341, ST414, ST343, ST262 and ST203) in VREfm, which were all designated as CC17 by eBURST algorithm. An outbreak of VREfm belonging to ST571 was found to happen within the neurology ward in this hospital. To our knowledge, this is the first report of ST6 (CC2) VREfs strains in China and the first outbreak report of VREfm strains belonging to ST571 around the world. Our data could offer important information for understanding the molecular features of VRE in China. Copyright © 2015 Elsevier B.V. All rights reserved.

  3. Inactivation Effect of Antibiotic-Resistant Gene Using Chlorine Disinfection

    Directory of Open Access Journals (Sweden)

    Takashi Furukawa

    2017-07-01

    Full Text Available The aim of this study was to elucidate the inactivation effects on the antibiotic-resistance gene (vanA of vancomycin-resistant enterococci (VRE using chlorination, a disinfection method widely used in various water treatment facilities. Suspensions of VRE were prepared by adding VRE to phosphate-buffered saline, or the sterilized secondary effluent of a wastewater treatment plant. The inactivation experiments were carried out at several chlorine concentrations and stirring time. Enterococci concentration and presence of vanA were determined. The enterococci concentration decreased as chlorine concentrations and stirring times increased, with more than 7.0 log reduction occurring under the following conditions: 40 min stirring at 0.5 mg Cl2/L, 20 min stirring at 1.0 mg Cl2/L, and 3 min stirring at 3.0 mg Cl2/L. In the inactivation experiment using VRE suspended in secondary effluent, the culturable enterococci required much higher chlorine concentration and longer treatment time for complete disinfection than the cases of suspension of VRE. However, vanA was detected in all chlorinated suspensions of VRE, even in samples where no enterococcal colonies were present on the medium agar plate. The chlorine disinfection was not able to destroy antibiotic-resistance genes, though it can inactivate and decrease bacterial counts of antibiotic-resistant bacteria (ARB. Therefore, it was suggested that remaining ARB and/or antibiotic-resistance gene in inactivated bacterial cells after chlorine disinfection tank could be discharged into water environments.

  4. Vananemise ilu ehk restaureerimisarhitekti roll / Ove Hidemark

    Index Scriptorium Estoniae

    Hidemark, Ove

    1997-01-01

    Restaureerimisarhitekti ülesannetest - ta ei pea otsustama üksnes tehniliste küsimuste üle, vaid arvestama ka sajandite vältel muutunud maitset, vana käsitöö erilist atmosfääri, toetama ehitisse koondatud ajaloolist elamust, nähes selles aja ja muutumise tervikut ning inimkäe mõju ja ajaloo sünteesi. Artikkel "Att behärska åldrandets skönhet eller om restaureringsarkitektens roll" rootsi ajakirjast "Kulturmiljövård", 1990, nr.4, lk.13-16

  5. Kujundusideid

    Index Scriptorium Estoniae

    2005-01-01

    Aet Pieli projekteeritud minimalistlik ja eestiaegses majas asuv köök. Vana muljega köök, mille mööbel on valmistatud firmas Moons. Kadri Pärtelpoja projekteeritud ehitusfirma NCC kinnisvaraosakonna paekiviseintega näidisköök. Köök Ivi-Els Schneideri kujundatud elamus. Hansperdis disainitud ja valmistatud noore perekonna erksates toonides köögimööbel. Arensis disainitud ja valmistatud seriaali "Kodu keset linna" köök. 22 värv. ill

  6. Tühjast pööningust ateljeekorteriks / Mari Koger

    Index Scriptorium Estoniae

    Koger, Mari

    2003-01-01

    Sisearhitekt M. Koger Nõmmel asuva 1920. aastatel ehitatud kivitrepikojaga kahekordse puumaja renoveerimistööde ajal kõrgemale tõstetud katuse alla ehitatud rõduga korterist. Maja renoveerimisprojekti tegi arhitekt Hannes Niineväli. Vannitoa valamu voolis kasepahast kunstnik Endel Saarepuu. Tema pliiatsijoonistused on elutoa seintel. Vana mööbli restaureeris Janno Jõe. Vanutatud puidust kööginurga valmistas käsitööna Kalmer Jäärats. Korteri plaan, 9 vaadet

  7. IBBY Eesti osakond 2007

    Index Scriptorium Estoniae

    2007-01-01

    IBBY Eesti osakond esitas Astrid Lindgreni mälestusauhinna kandidaatideks kirjanik Aino Perviku, illustraatorid Regina Lukk-Toompere ja Ilon Wiklandi. Paabeli Torni auhinna pälvis Arvo Valton komi kirjaniku Vladimir Timini noorsooromaani "Vana-Permi poisi Tikö seiklused" eesti keelde tõlkimise eest. IBBY aunimekirja esitati A. Kivirähk raamatuga "Leiutajateküla Lotte", kunstnik Jüri Mildeberg illustratsioonidega raamatule "Ruttu tuttu" ja tõlkija Piret Saluri Hannu Mäkela raamatu "Härra Huu teeb aiatööd" tõlkimise eest

  8. El pensamiento médico del doctor José Celestino Mutis. La Escuela de Medicina del Colegio Mayor de Nuestra Señora del Rosario en 1802.

    Directory of Open Access Journals (Sweden)

    Max Olaya Restrepo

    1968-03-01

    Full Text Available "Resulta muy difícil escribir Historia. Nunca se averigua con certeza de qué modo tuvieron lugar los sucesos, y las incertidumbres del historiador aumentan con la abundancia de documentos. Cuando un hecho es conocido por una referencia única, lo admitimos sin vacilación, pero al ofrecerse varios testimonios del mismo suceso, empiezan las perplejidades, pues no halla manera de armonizar las contradicciones evidentes. Para escribir Historia, se recurre a la vana imaginación".

  9. Occurrence and spread of antibiotic resistances in Enterococcus faecium.

    Science.gov (United States)

    Klare, Ingo; Konstabel, Carola; Badstübner, Dietlinde; Werner, Guido; Witte, Wolfgang

    2003-12-01

    Enterococci are the second to third most important bacterial genus in hospital infections. Especially Enterococcus (E.) faecium possesses a broad spectrum of natural and acquired antibiotic resistances which are presented in detail in this paper. From medical point of view, the transferable resistances to glycopeptides (e.g., vancomycin, VAN, or teicoplanin, TPL) and streptogramins (e.g., quinupristin/dalfopristin, Q/D) in enterococci are of special interest. The VanA type of enterococcal glycopeptide resistance is the most important one (VAN-r, TPL-r); its main reservoir is E. faecium. Glycopeptide-resistant E. faecium (GREF) can be found in hospitals and outside of them, namely in European commercial animal husbandry in which the glycopeptide avoparcin (AVO) was used as growth promoter in the past. There are identical types of the vanA gene clusters in enterococci from different ecological origins (faecal samples of animals, animal feed, patients in hospitals, persons in the community, waste water samples). Obviously, across the food chain (by GREF-contaminated meat products), these multiple-resistant bacteria or their vanA gene clusters can reach humans. In hospital infections, widespread epidemic-virulent E. faecium isolates of the same clone with or without glycopeptide resistance can occur; these strains often harbour different plasmids and the esp gene. This indicates that hospital-adapted epidemic-virulent E. faecium strains have picked up the vanA gene cluster after they were already widely spread. The streptogramin virginiamycin was also used as feed additive in commercial animal husbandry in Europe for more than 20 years, and it created reservoirs for streptogramin-resistant E. faecium (SREF). In 1998/1999, SREF could be isolated in Germany from waste water of sewage treatment plants, from faecal samples and meat products of animals that were fed virginiamycin (cross resistance to Q/D), from stools of humans in the community, and from clinical samples

  10. [19.-21. juulini korraldas Eesti Ulmeühing viiendat korda Eesti ulmehuviliste kokkutuleku "Estcon 2002". Tehti teatavaks ka aastaauhinnad Stalker laureaadid

    Index Scriptorium Estoniae

    2002-01-01

    Stalkeri laureaadid. Parim tõlkeromaan Roger Zelazny "Üksildane oktoobriöö" (Varrak, tlk. Juhan Habicht); parim kogumik Norman Spinradi "Suur lõõsk" (Skarabeus, tlk., koost. ja toim. Arvi Nikkarev); parim tõlkejutt "Katkuaastate päevikud" samast raamatust; parim algupärane raamat Indrek Hargla (õieti Marat Faizijev) kogumik "Pan Grpowski üheksa juhtumit" (Kuldsulg); parimaks algupärane jutustus Marat Faizijevi "Väendru" (Algernon, 2001, dets.); parim lühijutt Erkki Kõlu "Õnn kaalule, vana!" (Algernon, 2001, dets.)

  11. Lutikat : [luuletused] / Viktor Shibanov

    Index Scriptorium Estoniae

    Shibanov, Viktor

    2005-01-01

    Sisu: Lutikat / udmurdi keelest soome keelde tõlkinud Viktor Shibanov, Sara Hännikäinen ; Ylösalaisin kasvava puu / udmurdi keelest soome keelde tõlkinud Sara Hännikäinen, Hannu Oittinen ; Ovi / udmurdi keelest soome keelde tõlkinud Kari Sallamaa ; Lumest / udmurdi keelest eesti keelde tõlkinud koostöös autoriga Mihkel Kera ; Ringikujuline lõks ; Nulg ; Maja ; "Maa ühest otsast teise..." ; Vana ajaleht ; Neiu ja karu / udmurdi keelest eesti keelde tõlkinud Irina Orehhova, Kristiina Ehin

  12. Effect of Dietary Vanaspati Alone and in Combination with Stressors on Sero-biochemical Profile and Immunity in White Leghorn Layers

    OpenAIRE

    Raj, M. Alpha; Reddy, A. Gopala; Reddy, A. Rajasekhar; Adilaxmamma, K.

    2011-01-01

    A total of 160 White Leghorns of 20 wk age were divided randomly into eight groups. Groups 1, 3, 4 and 5 were fed basal feed and the rest were fed 5% vanaspati supplemented feed until 42 wk of age. From 42 to 54 wk, groups 3, 4 and 5 were fed 1% ferrous sulfate, 100 ppm chlorpyrifos (CPS) and 100 ppm cadmium, respectively, along with basal feed and groups 6, 7 and 8 were fed similar stressors, respectively, along with 5% vanaspati. Groups 1 and 2 served as controls for basal feed and 5% vanas...

  13. Uudised : Tüür Frankfurdi "Auftaktil". Motörhead ja Manson Soomes

    Index Scriptorium Estoniae

    2007-01-01

    Frankfurdi Vana Ooperi uut hooaega traditsiooniliselt sissejuhatava festivali "Auftakt" tänavusteks külalisteks on Erkki-Sven Tüür ja inglise tšellist Steven Isserlis, 28. aug.-1. okt. kestval festivalil mängitakse Tüüri 6. ja 7. (4. sümfooniat dirigeerib Paavo Järvi), 12. sept. kavas "Fata Morgana" ja "Oxymoron" ning Helena Tulve "Lumineux..." Olari Eltsi juhatusel. Inglise ansambli Motörhead kontserdist 11. dets. ja USA rokkari Marilyn Mansoni kontserdist 20. dets. Helsingis

  14. Neljatunnine teatrietendus Haimres / Kaupo Suuster, Tõnis Blank

    Index Scriptorium Estoniae

    Suuster, Kaupo

    2008-01-01

    Raplamaa harrastusteatrite festivalil Haimre kultuurimajas nähtud etendustest. Hageri näitering : A. Kivirähk "Atendaat", Valgu näitetrupp M.O.T.T. : A. Kitzbergi ainetel "Kosjasõit Valgu moodi", Kivi-Vigala näitering Sõbra-vägi : A. Liives "Need kauged ajad", Kodila näitetrupp : E. Bornhöhe "Tasuja", Rapla Teatritegemise Selts : "Lastejutt inetutele", Vana-Vigala näitetrupp Topelt-Kiiks : A. Kivirähk "Kalevipoeg", Kuimetsa näitetrupp : J. Orgussaar "Suured vaimud", Varbola näitetrupp V90 : "Eesti läbi aja"

  15. Rectal cancer presenting tumor thrombosis in the inferior vena cava and common iliac vein: case report

    Energy Technology Data Exchange (ETDEWEB)

    Rhee, Sun Jung; Park, Seong Jin; Lee, Hae Kyung; Yi, Boem Ha; Park, Sung Il; Hong, Soo Jin; Kim, Hee Kyung; Park, Jeong Mi [Soonchunhyang University Hospital, Bucheon (Korea, Republic of)

    2008-10-15

    We report the radiologic findings of a rectal carcinoma case with tumor thrombus in the inferior vana cava and left common iliac vein of a 48-year-old woman. The patient complained of swelling in the left leg and consequently underwent a lymphoscintigraphy, CT venography, abdominal CT, PET-CT, pelvis MRI, and ultrasound doppler. The rectal cancer was determined via a colonoscopy. The tissue biopsy of tumor thrombus in the IVC was done during insertion of IVC filter and poorly differentiated adenocarcinoma was revealed by pathology.

  16. Genetic characterization of glycopeptide-resistant enterococci of human and animal origin from mixed pig and poultry farms

    DEFF Research Database (Denmark)

    Jensen, Lars Bogø; Willems, R.J.L.; Van den Bogaard, A.E.

    2003-01-01

    Glycopeptide resistant enterococci (GRE) isolated from animals and humans were characterised using both AFPL typing and genetic characterisation of the glycopeptide resistance transposon Tn1546. All isolates were collected in 1997 when the glycopeptide avoparcin was still being used as growth...... promoter. All investigated animal isolates were from mixed pig and poultry farms in the Netherlands and the human isolated from the farmers of these farms. A total of 24 isolates were investigated. AFLP and Tn1546 typing revealed that both pig and poultry related enterococcal and vanA transposon genotypes...

  17. 17 eesti keraamikut Arkaadia teel / Airi Ligi

    Index Scriptorium Estoniae

    Ligi, Airi

    2003-01-01

    1. detsembril avatud ühisprojekt-näitusest Tartus "Põletatud savi antiigist tänapäevani", mille ekspositsioon koosnes Berliini Riigimuuseumi Vana-Kreeka originaalkeraamikast ja eesti keraamikute valdavalt selleks näituseks loodud taiestest. Illustratsioonidena Tartu Kultuurkapitali preemia pälvinud Annika Tederi vaasid "Santorini", punasefiguuriline pelike Apuuliast (4. saj. eKr.) ja mustafiguuriline amfora Atikast (510 eKr.). Viimane on foto TÜ Kunstimuuseumi varadest, mis tänaseni asuvad Vorone̓i Oblasti Kunstimuuseumis

  18. Typing of vancomycin-resistant enterococci from Danish hospitals

    DEFF Research Database (Denmark)

    Lester, Camilla H.; Olsen, Stefan S.; Schønheyder, Henrik Carl

    2009-01-01

    complex 17 (CC17) has been associated with nosocomial outbreaks in five continents. The aim of the present study was to elucidate the molecular epidemiology of VRE in Denmark. Methods: From January 2005 through October 2008, 61 vancomycin resistant enterococcal isolates causing invasive as well as non......-invasive infections were referred by seven of the 15 Danish departments of clinical microbiology to Statens Serum Institut. All isolates were identified to species level by PCR, MICs of vancomycin were determined (Trek Diagnostic Systems, UK), and the presence of vanA, vanB and vanC genes were detected by PCR. Multi...

  19. Books / Kadri Steinbach

    Index Scriptorium Estoniae

    Steinbach, Kadri

    2004-01-01

    Uutest muusikaraamatutest Elmar Arro "Vana aja muusikud", "Neeme Järvi. Encore!", J. Jürisson "Muusikud Kapid ja Suure-Jaani", V. Ojakäär "Omad viisid võõras väes", K. Pappel "Ooper Tallinnas 19. sajandil", V. Rumessen "Eduard Tubin", "Eduard Tubin. Rändavate vete ääres", A. Vahter "Eestikeelse muusikaterminoloogia kujunemine ja areng", A. Vahter "Kui mina alles nor veel olin", "A composition as a problem", "Cyrillus Kreek. helilooja", "Lepo Sumera", "Vardo Rumessen. Bibliograafia"

  20. Kurgiden aig = Kurgede aeg : [luuletused] / Nikolai Abramov

    Index Scriptorium Estoniae

    Abramov, Nikolai, 1961-

    2006-01-01

    Sisu: Kurgiden aig = Kurgede aeg ; Varastan sindai = Ootan sind ; Keväz̀ tuli = Kevad tuli ; Kibu = Valu ; "Miksh minä elän, miksh?..." = "Miks mina elan, miks?..." ; Vanh koir = Vana koer ; Ojat̀-randal = Ojati kaldal ; "Kül̀mi järven, kül̀mi jogen..." ; "Külmand järved, külmand jõed on..." ; "Uhostadud külä..." ; "Täis tuisand küla..." ; "Vaikastuiba lindud..." = "Vaikseks jäänud linnud..."

  1. Tagasi algusesse : Raekoja restaureerimise viis aastakümmet = Back to the Start : Five Decades of Restoration at the Town Hall / Teddy Böckler

    Index Scriptorium Estoniae

    Böckler, Teddy, 1930-2005

    2004-01-01

    1944. a. hävinud tornikiivri taastamisest. 1959. a. alanud raekoja arhitektuuri taastamisest. Kaaristu avati 1960. a. Interjööri taastamisest. 1971. a. alanud restaureerimisperioodist (sisekujundus: Leila Pärtelpoeg, Udo Umberg). 1991. a. alanud töödest: võlvkeldri taastamine, kohviku kujundamine. Tornikiivri ja tuulelipu Vana Toomas uuega asendamisest (1996). Lääneviilu tuulelipu uuendamisest (1999). 2000. a. alanud raekoja välispindade restaureerimisest, pööningu koristamisest. 2004. aastaks toimunud muudatustest interjööris

  2. Kultuurkapitali aastapreemiad 2009 / Liina Jänes

    Index Scriptorium Estoniae

    Jänes, Liina, 1977-

    2010-01-01

    Žürii liige kandidaatidest, preemia saanud objektidest, preemia määramise põhjendus. Peapreemia said Andres Alver, Veljo Kaasik ja Tiit Trummal Tallinna Vabaduse väljaku eest. Arhitektuuri objektipreemia võitis Uus- ja Vana Jahuladu Tallinnas (HG Arhitektuur, Hanno Grossschmidt, Tomomi Hayashi, Yoko Azukava), väikeobjekti preemia Lasva veetorn (põhiautor Veronika Valk), restaureerimise preemia Nõmme Saksa Lunastaja kirik (Fredi-Armand Tomps, Anne Koppel), sisearhitektuuri preemia AS Baltika büroo (Studio 3, Kolm Pluss Üks), tegevuspreemia Ilona Gurjanova

  3. Enterococcus faecalis as multidrug resistance strains in clinical isolates in Imam Reza Hospital in Kermanshah, Iran.

    Science.gov (United States)

    Mohammadi, F; Ghafourian, S; Mohebi, R; Taherikalani, M; Pakzad, I; Valadbeigi, H; Hatami, V; Sadeghifard, N

    2015-01-01

    The current study aimed to investigate the prevalence of vancomycin-resistant Enterococcus in E. faecalis and E. faecium and antimicrobial susceptibility patterns, then dominant genes responsible for vancomycin resistance were determined. For this propose, 180 clinical isolates of Enterococcus were subjected for identification and antibiotic susceptibility assay. Then, the gene responsible vancomycin resistant strains were determined. The results demonstrated the E. faecalis as a dominant Enterococcus. Resistance to erythromycin was dominant and multidrug resistance strains observed in E. faecalis. vanA was responsible for vancomycin resistance. In conclusion, a high rate of resistance to antibiotics in Enterococcus is clearly problematic, and a novel strategy is needed to decrease resistance in Enterococcus.

  4. Gastrointestinal Colonization with Vancomycin-Resistant Enterococci in Hospitalized and Outpatients

    Directory of Open Access Journals (Sweden)

    Elena Trajkovska-Dokic

    2015-10-01

    CONCLUSIONS: The prevalence of vancomycin resistant enterococci in Republic of Macedonia was 2-fold higher in hospitalized than in outpatients. VanA genotype was dominant in isolates of E. faecium and it was highly associated with the MIC values above the 256 mg/ml. Since most of the enterococcal infections are endogenous, there is a need for screening the colonization of patient’s intestinal flora with VRE at the hospital entry. Identification and genotyping of faecal enterococci, together with their susceptibility testing to vancomycin, could be useful marker for the infection control.

  5. Intestinal carriage of vancomycin-resistant enterococci in a community setting in Casablanca, Morocco.

    Science.gov (United States)

    Hannaoui, Imane; Barguigua, Abouddihaj; Serray, Bahija; El Mdaghri, Naima; Timinouni, Mohammed; Ait Chaoui, Ahmed; El Azhari, Mohamed

    2016-09-01

    This study aimed to determine the rate of intestinal carriage of vancomycin-resistant enterococci (VRE) and to perform a phenotypic and genotypic characterisation of VRE isolates in the community in Casablanca, Morocco. During 6 months in 2014, 113 faecal samples were examined for the presence of enterococci. Antibiotic susceptibility of the isolates was determined by the disk diffusion method. Phenotypic and genotypic species identification was performed, and the vanA, vanB and vanC genes were detected by PCR. Each bacterial isolate resistant to vancomycin was subjected to amplification and sequencing of the 16S rRNA gene. In total, 100 strains were collected from a community population of 80 persons; 55% of the isolates were identified as Enterococcus faecium and 45% as Enterococcus faecalis. The resistance profile showed that 88% of the strains were multiresistant. The rate of faecal carriage of VRE was 21% (n=21), among which 8 strains were E. faecalis (17.8% of all E. faecalis) and 13 strains were E. faecium (23.6% of all E. faecium). PCR analysis revealed that all of the strains were resistant to vancomycin owing to possession of the vanA gene. The emergence of VRE and the high rate of colonisation by multiresistant enterococci are alarming. Strict measures are required to control the further spread of these strains in the Moroccan community. Copyright © 2016 International Society for Chemotherapy of Infection and Cancer. Published by Elsevier Ltd. All rights reserved.

  6. Characterization of SCCmec and spa types of methicillin-resistant Staphylococcus aureus isolates from health-care and community-acquired infections in Kerman, Iran.

    Science.gov (United States)

    Fasihi, Yaser; Kiaei, Somayeh; Kalantar-Neyestanaki, Davood

    2017-12-01

    Spread of methicillin-resistant Staphylococcus aureus (MRSA) isolates is a worldwide problem. Molecular typing is a useful tool to understand MRSA epidemiology. Herein, we determined vancomycin-resistant, SCCmec and spa types among MRSA isolates recovered from healthcare and community-acquired infections in Kerman, Iran. A total of 170 S. aureus isolates were collected from different patients who were admitted to affiliated hospitals of Kerman University of Medical science. MRSA and vancomycin-resistant S. aureus (VRSA) isolates were detected by phenotypic methods. Polymerase chain reaction (PCR) technique was used for detection of mecA, vanA and vanB genes. Staphylococcal cassette chromosomemec (SCCmec) and spa typing were used for molecular typing of among MRSA isolates. Overall, 53% of isolates were considered as MRSA. Two MRSA isolates were resistant to vancomycin and vanA was detected in only one of VRSA isolates. SCCmec type III belonged to spa types t030 and t459 which they were the dominant spa types among community-associated MRSA (CA-MRSA) and healthcare-acquired MRSA (HA-MRSA) isolates. Our findings showed that the SCCmec type I and III spread from hospital settings to community, although the SCCmec type IV spread from community to healthcare systems. We have also reported VRSA isolates from hospitalized patients, therefore, appropriate policies should be enforced in order to prevent the spread of antibiotic resistance isolates in hospitals settings. Copyright © 2017 Ministry of Health, Saudi Arabia. Published by Elsevier Ltd. All rights reserved.

  7. Biological conversion of the aqueous wastes from hydrothermal liquefaction of algae and pine wood by Rhodococci

    Energy Technology Data Exchange (ETDEWEB)

    He, Yucai; Li, Xiaolu; Xue, Xiaoyun; Swita, Marie S.; Schmidt, Andrew J.; Yang, Bin

    2017-01-01

    In this study, R. opacus PD630, R. jostii RHA1, R. jostii RHA1 VanA-, and their co-culture were employed to convert hydrothermal liquefaction aqueous waste (HTLAW) into lipids. After 11 days, the COD reduction of algal-HTLAW reached 93.4% and 92.7% by R. jostii RHA1 and its mutant VanA-, respectively. Woody-HTLAW promoted lipid accumulation of 0.43 g lipid/g cell dry weight in R. opacus PD630 cells. Additionally, the total number of chemicals in HTLAW decreased by over 1/3 after 7 days of coculture, and 0.10 g/L and 0.46 g/L lipids were incrementally accumulated in the cellular mass during the fermentation of wood- and algal-HTLAW, respectively. The GC-MS data supported that different metabolism pathways were followed when these Rhodococci strains degraded algae- and woody-HTLAW. These results indicated promising potential of bioconversion of under-utilized carbon and toxic compounds in HTLAW into useful products by selected Rhodococci.

  8. Transferable Antibiotic Resistances in Marketed Edible Grasshoppers (Locusta migratoria migratorioides).

    Science.gov (United States)

    Osimani, Andrea; Garofalo, Cristiana; Aquilanti, Lucia; Milanović, Vesna; Cardinali, Federica; Taccari, Manuela; Pasquini, Marina; Tavoletti, Stefano; Clementi, Francesca

    2017-05-01

    Grasshoppers are the most commonly eaten insects by humans worldwide, as they are rich in proteins and micronutrients. This study aimed to assess the occurrence of transferable antibiotic resistance genes in commercialized edible grasshoppers. To this end, the prevalence of 12 selected genes [aac(6')-Ie aph(2″)-Ia, blaZ, erm(A), erm(B), erm(C), mecA, tet(M), tet(O), tet(S), tet(K), vanA, vanB] coding for resistance to antibiotics conventionally used in clinical practice was determined. The majority of samples were positive for tet(M) (70.0%), tet(K) (83.3%) and blaZ (83.3%). A low percentage of samples were positive for erm(B) (16.7%), erm(C) (26.7%), and aac(6')-Ie aph(2″)-Ia (13.3%), whereas no samples were positive for erm(A), vanA, vanB, tet(O), and mecA. Cluster analysis identified 4 main clusters, allowing a separation of samples on the basis of their country of origin. © 2017 Institute of Food Technologists®.

  9. Mixed Single/Double Precision in OpenIFS: A Detailed Study of Energy Savings, Scaling Effects, Architectural Effects, and Compilation Effects

    Science.gov (United States)

    Fagan, Mike; Dueben, Peter; Palem, Krishna; Carver, Glenn; Chantry, Matthew; Palmer, Tim; Schlacter, Jeremy

    2017-04-01

    It has been shown that a mixed precision approach that judiciously replaces double precision with single precision calculations can speed-up global simulations. In particular, a mixed precision variation of the Integrated Forecast System (IFS) of the European Centre for Medium-Range Weather Forecasts (ECMWF) showed virtually the same quality model results as the standard double precision version (Vana et al., Single precision in weather forecasting models: An evaluation with the IFS, Monthly Weather Review, in print). In this study, we perform detailed measurements of savings in computing time and energy using a mixed precision variation of the -OpenIFS- model. The mixed precision variation of OpenIFS is analogous to the IFS variation used in Vana et al. We (1) present results for energy measurements for simulations in single and double precision using Intel's RAPL technology, (2) conduct a -scaling- study to quantify the effects that increasing model resolution has on both energy dissipation and computing cycles, (3) analyze the differences between single core and multicore processing, and (4) compare the effects of different compiler technologies on the mixed precision OpenIFS code. In particular, we compare intel icc/ifort with gnu gcc/gfortran.

  10. A prism of excellence: The Charleston Veterans Administration Nursing Academic Partnership.

    Science.gov (United States)

    Coxe, D Nicole; Conner, Brian T; Lauerer, Joy; Skipper, Janice; York, Janet; Fraggos, Mary; Stuart, Gail W

    2016-01-01

    The Veterans Administration (VA) has been committed to academic affiliate training partnerships for nearly 70 years in efforts to enhance veteran-centric health care. One such effort, the VA Nursing Academy (VANA) program, was developed in 2007 in response to the nationwide nursing shortage and began as a five-year pilot with funding competitively awarded to 15 partnerships between local VA medical centers and schools of nursing. The VANA program evolved into the VA Nursing Academic Partnership (VANAP) program following the initial pilot. This article describes the development and evolution of the Charleston VANAP, which includes the Ralph H Johnson VA Medical Center (RHJ VAMC) and the Medical University of South Carolina College of Nursing (MUSC CON). The VA Office of Academic Affiliations (OAA) funded a large portion of the initial five years of the Charleston VANAP. Once the national funding source ceased, the RHJ VAMC and the MUSC CON entered into a Memorandum of Understanding (MOU) to offer in-kind contributions to the partnership. The Charleston VANAP is the only program in the nation to offer three different nurse trainee programs and this article highlights some of the more notable achievements from each program. The Charleston VANAP is a comprehensive partnership between the RHJ VAMC and the MUSC CON that truly demonstrates a commitment to assure that the very best care be provided to Veterans, our Nation's heroes. Copyright © 2016 Elsevier Inc. All rights reserved.

  11. Absence of vancomycin-resistant enterococci among highly ESBL-positive crows (Corvus splendens foraging on hospital waste in Bangladesh

    Directory of Open Access Journals (Sweden)

    Badrul Hasan

    2015-12-01

    Full Text Available Background: Vancomycin-resistant enterococci (VRE have emerged as a growing problem in hospitals; however, domesticated animals, poultry, and wild birds are acting as potential reservoirs. There is a knowledge gap in the Epidemiology of VRE from Bangladesh. Methods: To study the prevalence of VRE and the mechanisms of resistance implicated among wild birds, 238 fecal samples were collected in 2010 from house crows (Corvus splendens foraging on hospital waste in Bangladesh. Fecal samples were screened by analyzing color change in broth and screening for vanA and vanB resistant genes by PCR. Results: Neither vanA nor vanB genes were detected from the fecal samples. The house crow does not seem to constitute a reservoir for VRE. Conclusion: The zero prevalence is an indication that foraging on hospital waste does not constitute a major risk of VRE carriage in house crows and this is the first study to focus on the prevalence of VRE from wild birds in Bangladesh.

  12. Clonal dissemination of VanA-type glycopeptide-resistant Enterococcus faecalis between hospitals of two cities located 100 km apart

    Directory of Open Access Journals (Sweden)

    M.L. Moretti

    2004-09-01

    Full Text Available Nosocomial dissemination of glycopeptide-resistant enterococci represents a major problem in hospitals worldwide. In Brazil, the dissemination among hospitals in the city of São Paulo of polyclonal DNA profiles was previously described for vancomycin-resistant Enterococcus faecium. We describe here the dissemination of VanA phenotype E. faecalis between two hospitals located in different cities in the State of São Paulo. The index outbreak occurred in a tertiary care university hospital (HCUSP in the city of São Paulo and three years later a cluster caused by the same strain was recognized in two patients hospitalized in a private tertiary care hospital (CMC located 100 km away in the interior of the state. From May to July 1999, 10 strains of vancomycin-resistant E. faecalis were isolated from 10 patients hospitalized in the HCUSP. The DNA genotyping using pulsed-field gel electrophoresis (PFGE showed that all isolates were originated from the same clone, suggesting nosocomial dissemination. From May to July 2002, three strains of vancomycin-resistant E. faecalis were isolated from two patients hospitalized in CMC and both patients were colonized by the vancomycin-resistant Enterococcus in skin lesions. All isolates from CMC and HCUSP were highly resistant to vancomycin and teicoplanin. The three strains from CMC had minimum inhibitory concentration >256 µg/ml for vancomycin, and 64 (CMC 1 and CMC 2 and 96 µg/ml (CMC 3 for teicoplanin, characterizing a profile of VanA resistance to glycopeptides. All strains had the presence of the transposon Tn1546 detected by PCR and were closely related when typed by PFGE. The dissemination of the E. faecalis VanA phenotype among hospitals located in different cities is of great concern because E. faecalis commonly colonizes the gastrointestinal tract of patients and healthy persons for periods varying from weeks to years, which, together with the persistence of vancomycin-resistant Enterococcus in

  13. Clonal dissemination of VanA-type glycopeptide-resistant Enterococcus faecalis between hospitals of two cities located 100 km apart

    Directory of Open Access Journals (Sweden)

    Moretti M.L.

    2004-01-01

    Full Text Available Nosocomial dissemination of glycopeptide-resistant enterococci represents a major problem in hospitals worldwide. In Brazil, the dissemination among hospitals in the city of São Paulo of polyclonal DNA profiles was previously described for vancomycin-resistant Enterococcus faecium. We describe here the dissemination of VanA phenotype E. faecalis between two hospitals located in different cities in the State of São Paulo. The index outbreak occurred in a tertiary care university hospital (HCUSP in the city of São Paulo and three years later a cluster caused by the same strain was recognized in two patients hospitalized in a private tertiary care hospital (CMC located 100 km away in the interior of the state. From May to July 1999, 10 strains of vancomycin-resistant E. faecalis were isolated from 10 patients hospitalized in the HCUSP. The DNA genotyping using pulsed-field gel electrophoresis (PFGE showed that all isolates were originated from the same clone, suggesting nosocomial dissemination. From May to July 2002, three strains of vancomycin-resistant E. faecalis were isolated from two patients hospitalized in CMC and both patients were colonized by the vancomycin-resistant Enterococcus in skin lesions. All isolates from CMC and HCUSP were highly resistant to vancomycin and teicoplanin. The three strains from CMC had minimum inhibitory concentration >256 µg/ml for vancomycin, and 64 (CMC 1 and CMC 2 and 96 µg/ml (CMC 3 for teicoplanin, characterizing a profile of VanA resistance to glycopeptides. All strains had the presence of the transposon Tn1546 detected by PCR and were closely related when typed by PFGE. The dissemination of the E. faecalis VanA phenotype among hospitals located in different cities is of great concern because E. faecalis commonly colonizes the gastrointestinal tract of patients and healthy persons for periods varying from weeks to years, which, together with the persistence of vancomycin-resistant Enterococcus in

  14. Tammsaare kirjanduspreemiate laureaadid / šaržid: Hugo Hiibus

    Index Scriptorium Estoniae

    1996-01-01

    Preemiat on antud välja alates 1978. a. Preemia saajad : Helene Siimisker. 'A. H. Tammsaare pildikroonika eluteest ja tööst'. (1977); Juhan Peegel. 'Ma langesin esimesel sõjasuvel'. (1978); Ilmar Sikemäe. 'Kirjad Vargamäelt'. (1979); Vello Lattik. 'Kuus head inimest'. (1980); Veera Saar. 'Aprillipäev'. (1981); Mats Traat. 'Karukell, kurvameelsuse rohi'. (1982); Raimond Kaugver. 'Vana mees tahab koju'. (1983); Mari Saat. 'Õun valguses ja varjus'. (1984); Viivi Luik. 'Seitsmes rahukevad'. (1985); Heino Kiik. 'Elupadrik'. (1986); Luise Vaher. 'Põlev pärisosa'. (1987); Hando Runnel. 'Laulud eestiaegsetele meestele'. (1988); Arvo Valton. 'Masendus ja lootus'. (1989); Kalju Lepik. 'Rukkilille murdmise laul'. (1990); Illar Mõttus. Dramatiseering 'Jumalaga Vargamäe'. (1991); Leelo Tungal. 'Barbara ja suvekoerad'. (1992); Aili Viitar. 'Kummelimuru'. (1993); Albert Uustulnd. 'Rajud ei rauge', 'Lummav meri'. (1994)

  15. "Seal ta nüüd lamabki, ümmarguses hauas..." : [luuletused] / Paavo Haavikko

    Index Scriptorium Estoniae

    Haavikko, Paavo, 1931-

    2008-01-01

    Sisu: "Seal ta nüüd lamabki, ümmarguses hauas..." ; "See, kes ütleb, et mees läheb sinna, kuhu tubakaski, ei tea..." ; Ootamatu toost ; "Moskva..." ; "Inimese parim omadus on tema lühiajalisus..." ; "Ööbik ei suuda laulda. Ta ei suuda olla laulmata..." ; "Hääletan kevade poolt, sügis valitakse, talv moodustab valitsuse..." ; "Ümmargune kuu, lahtiriietuv naine, püstine voolav vesi..." / tlk. Hasso Krull. "Kuulen lõbusat lugu, see teeb mind kurvaks..." ; "Tuleb sügis, õunapirukad, mälestused, mille pääl on..." ; "Vana mees on rääkimisest väsinud..." ; "Siin julmas maailmas on asjatu paludagi..." ; "Võid kindel olla, et see on tema ise..." ; "On palju tarku mehi, aga samas ei ühtki..." / tlk. Lauri Kitsnik

  16. Mis tuleb

    Index Scriptorium Estoniae

    2004-01-01

    Säästva Renoveerimise Infokeskuses Tallinnas Liivalaia 19a 4. XI vanade uste kordategemise ja 27. XI vana mööbli korrastamise ja restaureerimise töötuba; Tallinnas Väike-Patarei 3 insener Heino Uuetalu loengud: 11. XI "Vanade majade soklikorruste korrashoid ja renoveerimine", 25. XI "Vanade majade välisseinte, vahelagede, pööningute renoveerimine ja korrashoid". Tartu Kunstimajas Eesti Nahakunstnike Liidu korraldatud ehtenäitus "Ehe on teade maailmale meist endist". Viinistu Kunstimuuseumi tünnigaleriis Aarne Vasara mälestusnäitus. Viljandi Muuseumis näitus "Meleski klaas", mille koostasid Urmas Leemet, Heiti Hakmann ja Ville Dreving

  17. Eesti Kultuurkapital : 1997. aasta toetuse teine jaotus

    Index Scriptorium Estoniae

    1997-01-01

    Helikunsti sihtkapitalilt Aurika Gergelezhiule osalemiseks IAMLi aastakonverentsil Genfis; Kirjanduse sihtkapitalilt EKM Arhiivraamatukogule ajakirjanduse sisu retrospektiivse bibliograafia koostamiseks, TÜ Raamatukogule maali "Pent Nurmekund" (1977) ostmiseks; Valdkondadevaheliselt sihtkapitalilt Urve Koldele restaureerimisalaseks praktikaks Saksamaal, Kaie Kuslapile ja Anneli Sepale osalemiseks IAMLi aastakonverentsi Genfis, EAR-ile noorte infotöötajate täiendõppeks Helsingis ja kataloogi "Köiteuunikume EARis" trükikuludeks, ERÜ-le ajakirja "Raamatukogu" honorarifondiks, Eesti Raamatuühingule VI Viljandi vanaraamatupäevade läbiviimiseks, Holstre ja Rõngu Raamatukogule arvuti ja tarkvara ostmiseks, Räpina Linna Keskraamatukogule raamatukogutarkvara ostmiseks, Vana-Vigala Raamatukogule paljundusmasina ostmiseks, Varbola Raamatukogule arvuti ja printeri ning Vastemõisa Külaraamatukogule arvuti ja modemi ostmiseks

  18. Molecular characterization of vancomycin-resistant Enterococcus faecium isolates from Bermuda.

    Directory of Open Access Journals (Sweden)

    Patrick Eberechi Akpaka

    Full Text Available Molecular characteristics of vancomycin resistant enterococci isolates from Bermuda Island is currently unknown. This study was conducted to investigate phenotypic and genotypic characteristics of VRE isolates from Bermuda Island using the chromogenic agar, E-tests, polymerase chain reaction (PCR, pulsed-field gel electrophoresis (PFGE and multilocus sequence typing (MLST. Eighteen E. faecium isolates were completely analyzed and were all resistant to vancomycin, susceptible to linezolid and quinupristin/dalfopristin, positive for vanA and esp genes. The MLST analysis confirmed most isolates were of the sequence types linked to clonal complex 17 (CC17 that is widely associated with outbreaks in hospitals. Infection control measures, antibiotic stewardship, and surveillance activities will continue to be a priority in hospital on the Island.

  19. El rodeo de la huella en la filosofía de Paul Ricœur

    OpenAIRE

    Mena Malet, Patricio; Universidad Alberto Hurtado

    2005-01-01

    El carácter itinerante de la obra filosófica de Ricœur nos encamina por un largo rodeo de proyectos abovedados que, remitiéndose unos a otros, anteponen a la vana ilusión de quedar bien definidos y acabados, una muy esperanzadora experiencia fenomenológica y hermenéutica: el trabajo de desentrañar las más variadas huellas de la alteridad; la posibilidad de inventar nuevas vías de acceso a lo insólito. La figura laberíntica con la que el filósofo francés ha revolucionado la filosofía reflexiva...

  20. 2006. aasta arhitektuurikonkursid / Karen Jagodin

    Index Scriptorium Estoniae

    Jagodin, Karen, 1982-

    2006-01-01

    Toodud ka 2006. a. konkursside tulemused: Eesti Rahva Muuseumi uus hoone, Tartu Ida t. lastead, Kiviõli linnaväljak, Tartu Lutsu t. korterelamu fassaadilahendus, Paide spordihall, Pärnu piirivalvekordoni hoone, Tartu Rebase t. kortermajade kompleks, Viljandi Maagümnaasiumi juurdeehitus, Veneetsia X arhitektuuribiennaali Eesti kuraatorinäitus, betoonehitis 2005, Estonia Seltsi vana teatrimaja asukoha tähistamine, TTÜ uus raamatukogu, Maakri kvartali arhitektuurikonkursi I etapp, Viljandi Uku t. 1A Lidli kauplusehoone ja südalinna kvartali detailplaneering, Pärnu jõeäärse keskuse ideeplaneering, Karksi-Nuia keskväljak ja roheala, terasehitis 2006, Edela-Eesti loodus- ja keskkonnahariduskeskuse ehk ökomaja eskiisprojekt, puitehitis 2006

  1. Identification and molecular characterization of Van A-type vancomycin-resistant Enterococcus faecalis in Northeast of Brazil

    Directory of Open Access Journals (Sweden)

    Marinalda Anselmo Vilela

    2006-11-01

    Full Text Available The isolation of vancomycin resistant enterococci (VRE in Brazil has rapidly increased, following the world wide tendency. We report in the present study the first isolation of vancomycin resistant Enterococcus faecalis (VRE in the Northeast of Brazil. The four VRE isolates were characterized for antimicrobial susceptibility, genotypic typing by macro restriction of chromosomal DNA followed by pulsed-field gel electrophoresis and for characterization of the Tn1546-like element and plasmid contents. The isolates showed resistance to multiple antibiotics and a single genotype profile, suggesting the dissemination of a single clone among the patients. Tn1546 associated to genetic elements as plasmids shows the importance of infection control measures to avoid the spreading of glycopetide resistance by conjugative transfer of VanA elements.

  2. Semblanza de un maestro: Alfredo Carrea Henao

    Directory of Open Access Journals (Sweden)

    Tiberio Alvarez Echeverri

    1994-02-01

    Full Text Available

    Fue maestro a pesar de su timidez y retraimiento. Prefería los silencios y el ejemplo a la palabra vana. Se mantuvo alejado de los vaivenes sociales y de las conveniencias publicitarias. Sin buscarlos recibió placas y reconocimientos: por el retrato de la Pola, cum laude por su tesis, año sabático por su investigación en fiebre amarilla, Cruz de Boyacá y Premio Nacional de Medicina por su trabajo, Profesor Distinguido de la Universidad por su labor formativa, Orden del Arriero por sus conocimientos de mulas y enjalmas.

  3. Characterization of vancomycin-resistant and vancomycin-susceptible Enterococcus faecium isolates from humans, chickens and pigs by RiboPrinting and pulsed-field gel electrophoresis

    DEFF Research Database (Denmark)

    Hammerum, Anette Marie; Fussing, Vivian; Aarestrup, Frank Møller

    2000-01-01

    Forty-eight vancomycin-resistant and 35 vancomycin-sensitive Danish Enterococcus faecium isolates obtained from pigs, chickens and humans, as well as the human vanA reference isolate BM4147, were characterized by EcoRI RiboPrinting and Smal pulsed-field gel electrophoresis. RiboPrinting of the 84...... isolates yielded 40 types whereas PFGE-typing yielded 57 types discriminated by differences in more than three bands. By molecular typing, both clonal spread of E. faecium as well as horizontal transmission of Tn1546 between animals and humans was supported. Furthermore, it was found that the population...... of E. faecium spreads freely between the animal and human reservoir....

  4. Use of antimicrobial growth promoters in food animals and Enterococcus faecium resistance to therapeutic antimicrobial drugs in Europe

    DEFF Research Database (Denmark)

    Wegener, Henrik Caspar; Aarestrup, Frank Møller; Jensen, Lars Bogø

    1999-01-01

    , clear evidence of a health risk was not available. Accumulating evidence now indicates that the use of the glycopeptide avoparcin as a growth promoter has created in food animals a major reservoir of Enterococcus faecium, which contains the high level glycopeptide resistance determinant vanA, located......Supplementing animal feed with antimicrobial agents to enhance growth has been common practice for more than 30 years and is estimated to constitute more than half the total antimicrobial use worldwide. The potential public health consequences of this use have been debated; however, until recently...... among the growth promoters, and a huge animal reservoir of resistant E. faecium has already been created, posing a new public health problem....

  5. Short outlines of books by Estonian authors / Rutt Hinrikus, Janika Kronberg

    Index Scriptorium Estoniae

    Hinrikus, Rutt, 1946-

    2001-01-01

    Arvo Valton. Leidik; Mats Traat. Valge lind; Mats Traat. Harala elulood; Kärt Hellerma. Kassandra; Uku Masing. Luule 1.; Ilmar Vene. Trotsija : katse mõista Uku Masingut; Eesti identiteet ja iseseisvus / koost. A. Bertricau; Ene Mihkelson. Kaalud ei kõnele : valitud luuletusi 1967-1977; Mehis Heinsaar. Vanameeste näppaja; Eesti kirjanduslugu / Epp Annus, Luule Epner, Ants Järv jt.; Aarne Ruben. Volta annab kaeblikku vilet; Mart Kivastik. Näidendid; Kaardipakk / Jürgen Rooste, Karl Martin Sinijärv, Triin Soomets, Elo Viiding, Asko Künnap; Hasso Krull. Kornukoopia : sada luuletust; Tiia Toomet. Vana aja koolilood; Aidi Vallik. Kuidas elad, Ann?; Enn Vetemaa. Neitsist sündinud

  6. Is Bitcoin here to stay?- A case study on cryptocurrency, Bitcoin

    OpenAIRE

    Abdi, Brittemira

    2014-01-01

    Begreppet pengar har funnits sedan vi började använda varor och råvaror som betalningsmedel. I tusentals år har människor tagit för vana att använda pengar till att handla med varor och tjänster. Utvecklingen av pengar som ett verktyg för handel började med användandet av kakao frön, snäckor och andra föremål som ansågs värdefulla, som sen har utvecklats till användningen av ädla metaller, som guld och silver. Under de senaste århundradena har vi använt fiat valuta och nu, med utvecklingen av...

  7. Antimicrobial Resistance Genes in Pigeons from Public Parks in Costa Rica.

    Science.gov (United States)

    Blanco-Peña, K; Esperón, F; Torres-Mejía, A M; de la Torre, A; de la Cruz, E; Jiménez-Soto, M

    2017-11-01

    Antimicrobial resistance is known to be an emerging problem, but the extent of the issue remains incomplete. The aim of this study was to determine the presence or absence of nine resistance genes (bla TEM , catI, mecA, qnrS, sulI, sulII, tet(A), tet(Q), vanA) in the faeces of 141 pigeons from four urban parks in Alajuela, Guadalupe, Tres Ríos and San José in Costa Rica. The genes were identified by real-time PCR directly from enema samples. About 30% of the samples were positive for genes catI and sulI; between 13% and 17% were positive for qnrS, sulII, tet(A) and tet(Q); and 4% were positive for bla TEM . The mecA and vanA genes were not detected. The average of antimicrobial resistance genes detected per pigeon was 2. Eight different patterns of resistance were identified, without differences in the sampling areas, being the most common pattern 2 (sulII positive samples). During rainy season, the genes more frequently found were sulI and tet(A). In conclusion, the urban inhabiting pigeons tested are currently carrying antimicrobial resistance genes, potentially acting as reservoirs of resistant bacteria and vectors to humans. To the authors' knowledge, this is the first study carried out on direct detection of resistance genes in the digestive metagenomes of pigeons. © 2017 The Authors. Zoonoses and Public Health Published by Blackwell Verlag GmbH.

  8. El tema de la ceguera en la literatura religiosa de Lope

    Directory of Open Access Journals (Sweden)

    Lezcano Tosca, Hugo

    2004-12-01

    Full Text Available In baroque literature, el desengaño is a commonplace that gets a large development. El desengaño means that man realizes that everything he can see is «vanas sombras». In this article, the question of blindness in Lope de Vega's religious poetry is studied through three different aspects: first, blindness of the self in religious poetry is considered as a transformation «a lo divino» of petrarchist lover blindness. Secondly, the divine call is studied in some literary and religious texts, like Saint August Confessions, or Lope de Vega's Soliloquios. Finally, the different methods used by the Society of Jesus to finish with man's blindness (compositio loci, emblems are analysed.En la literatura barroca, el desengaño se convierte en un lugar común que recibe un amplio desarrollo. El desengaño consiste en que el hombre se da cuenta de que lo que ve con sus ojos es falso, puesto que las bellezas de la tierra sólo son «vanas sombras». En este artículo se estudia el tema de la ceguera en la literatura religiosa de Lope, teniendo en cuenta tres aspectos diferentes: en primer lugar, se analiza la ceguera del yo de la literatura religiosa como transformación «a lo divino» de la ceguera del enamorado petrarquista. En segundo lugar, se estudia en qué consiste la llamada divina en algunos textos religiosos y literarios, como las Confesiones de San Agustín o los Soliloquios de Lope. Por último, se estudian los métodos que emplea la Compañía de Jesús (composición de lugar, libros de emblemas para acabar con la ceguera del hombre.

  9. Amores intertextuales y parodias posmodernistas: Vicente Molina Foix y la poesía

    Directory of Open Access Journals (Sweden)

    Gala, Candelas

    2010-12-01

    Full Text Available Taking as a point of departure the two poetry books, Los espías del realista y Vanas penas de amor, that Vicente Molina Foix published in 1990 and 1998, respectively, this essay analyzes the role of the ironic glance the poetic speaker casts on the literary, artistic and cultural models firmly rooted in the analytical-referential system of tradition. A careful reading of specific poems from both collections reveals Molina Foix’s use of parodic and inter-textual strategies to debunk the monumentality of such discourses, not so much to ridicule or do away with them, but to show their incongruence in the modern context and at the level of the individual person.Tomando como punto de partida los dos libros de poesía, Los espías del realista y Vanas penas de amor, que Vicente Molina Foix publica en 1990 y 1998, respectivamente, este ensayo analiza el papel de la mirada irónica que el hablante poético dirige a los modelos literarios, artísticos y culturales sólidamente establecidos en el sistema analítico y referencial de la tradición. La lectura atenta de poemas específicos procedentes de ambas colecciones revela el uso que hace Molina Foix de estrategias paródicas e intertextuales para desbancar la monumentalidad de dichos discursos con el fin no tanto de descartarlos y ridiculizarlos, sino más bien de mostrar su incongruencia en el contexto moderno y a nivel de la persona individual.

  10. Two-tier approach combining molecular and culture-based techniques for optimized detection of vancomycin-resistant enterococci.

    Science.gov (United States)

    Both, Anna; Franke, Gefion C; Mirwald, Nadine; Lütgehetmann, Marc; Christner, Martin; Klupp, Eva-Maria; Belmar Campos, Cristina; Büttner, Henning; Aepfelbacher, Martin; Rohde, Holger

    2017-08-24

    Given constantly high or even rising incidences of both colonization and infection with vancomycin-resistant enterococci (VRE), timely and accurate identification of carriers in high-risk patient populations is of evident clinical importance. In this study, a two-tier approach consisting of PCR-based screening and cultural confirmation of positive results is compared to the conventional approach solely based on culture on selective media. The 2-tier strategy was highly consistent with the conventional approach, and was found to possess high sensitivity and specificity (93.1% and 100%, respectively). The introduction of the PCR-based combined VRE screening approach significantly (P<0.0001) reduced median overall time to result by 44.3hours. The effect was found to be most pronounced in VRE negative samples. Positive vanA PCR was highly consistent with culture (PPV: 92.0%, 95% CI: 72.5-98.6%, NPV: 99.6%, 95% CI: 98.9-99.6%), thus allowing for preliminary reporting of VRE detection. In contrast, a vanB positive PCR does not allow for preliminary reporting (PPV: 58.5%, 95% CI: 44.2-71.6%, NPV: 99.8%, 95% CI: 99.2-100%). The introduction of a molecular assay for rapid detection of VRE from rectal swabs combined with cultural confirmation proved to be reliable and time saving, especially in a setting of low VRE prevalence and predominance of vanA positive strains. Copyright © 2017 Elsevier Inc. All rights reserved.

  11. Multidrug-resistant enterococci in animal meat and faeces and co-transfer of resistance from an Enterococcus durans to a human Enterococcus faecium.

    Science.gov (United States)

    Vignaroli, Carla; Zandri, Giada; Aquilanti, Lucia; Pasquaroli, Sonia; Biavasco, Francesca

    2011-05-01

    Forty-eight isolates resistant to at least two antibiotics were selected from 53 antibiotic-resistant enterococci from chicken and pig meat and faeces and analysed for specific resistance determinants. Of the 48 multidrug-resistant (MDR) strains, 31 were resistant to two antibiotics (29 to erythromycin and tetracycline, 1 to erythromycin and vancomycin, 1 to vancomycin and tetracycline), 14 to three (erythromycin, tetracycline and vancomycin or ampicillin) and 3 to four (erythromycin, vancomycin, ampicillin and gentamicin). erm(B), tet(M), vanA and aac (6')-Ie aph (2'')-Ia were the antibiotic resistance genes most frequently detected. All 48 MDR enterococci were susceptible to linezolid and daptomycin. Enterococcus faecalis (16), Enterococcus faecium (8), Enterococcus mundtii (2) and Enterococcus gallinarum (1) were identified in meat, and E. faecium (13) and Enterococcus durans (13) in faeces. Clonal spread was not detected, suggesting a large role of gene transfer in the dissemination of antibiotic resistance. Conjugative transfer of resistance genes was more successful when donors were enterococcal strains isolated from faeces; co-transfer of vanA and erm(B) to a human E. faecium occurred from both E. faecium and E. durans pig faecal strains. These data show that multidrug resistance can be found in food and animal species other than E. faecium and E. faecalis, and that these species can efficiently transfer antibiotic resistance to human strains in inter-specific matings. In particular, the occurrence of MDR E. durans in the animal reservoir could have a role in the emergence of human enterococcal infections difficult to eradicate with antibiotics.

  12. Molecular characterization of vancomycin-resistant Enterococcus faecium strains isolated from carriage and clinical samples in a tertiary hospital, Turkey.

    Science.gov (United States)

    Gozalan, Aysegul; Coskun-Ari, Fatma Filiz; Ozdem, Birsen; Unaldi, Ozlem; Celikbilek, Nevreste; Kirca, Fisun; Aydogan, Sibel; Muderris, Tuba; Guven, Tumer; Acikgoz, Ziya Cibali; Durmaz, Riza

    2015-07-01

    This study aimed to determine the presence of vancomycin resistance (vanA and vanB) and virulence genes (esp, asa1, gelE, ace, hyl, cylA, cpd and ebpA) in vancomycin-resistant Enterococcus faecium (VREfm) strains and to analyse the clonal relationships among the strains. E. faecium strains were identified from rectal and clinical specimens by biochemical tests and the API-20 Strep kit. Susceptibility testing was performed using disc-diffusion and broth-dilution methods. PFGE was used for molecular typing of the VREfm strains. The vancomycin resistance and virulence genes were amplified by two-step multiplex PCR. All 55 VREfm isolates were resistant to penicillin G, ampicillin and high-level gentamicin but were susceptible to quinupristin/dalfopristin and linezolid. Multiplex PCR analysis indicated that all isolates harboured vanA and that 41 (75 %) were positive for virulence genes. The esp gene was the most common virulence factor and was detected in nine (41 %) invasive and 32 (96.7 %) non-invasive isolates. Multiple virulence genes were observed only in two non-invasive isolates; one harboured esp and ebpA and the other harboured esp, ebpA, asa1, gelE and cpd. PFGE typing yielded 16 different types, seven of which were clusters with two to 14 strains each. The clustering rates of the rectal swab, blood and urine isolates were 72.7 %, 61.5 % and 87.5 %, respectively. The genetic similarity observed among the VREfm isolates indicated cross-transmission in the hospital. Further studies on the virulence factors present in the strains might provide insight into the acquisition of these traits and their contribution to increased prevalence of VREfm.

  13. Clinical relevance of molecular identification of microorganisms and detection of antimicrobial resistance genes in bloodstream infections of paediatric cancer patients.

    Science.gov (United States)

    Carlesse, Fabianne; Cappellano, Paola; Quiles, Milene Gonçalves; Menezes, Liana Carballo; Petrilli, Antonio Sérgio; Pignatari, Antonio Carlos

    2016-09-01

    Bloodstream infections (BSIs) are the major cause of mortality in cancer patients. Molecular techniques are used for rapid diagnosis of BSI, allowing early therapy and improving survival. We aimed to establish whether real-time quantitative polymerase chain reaction (qPCR) could improve early diagnosis and therapy in paediatric cancer patients, and describe the predominant pathogens of BSI and their antimicrobial susceptibility. Blood samples were processed by the BACTEC system and microbial identification and susceptibility tests were performed by the Phoenix system. All samples were screened by multiplex 16 s rDNA qPCR. Seventeen species were evaluated using sex-specific TaqMan probes and resistance genes blaSHV, blaTEM, blaCTX, blaKPC, blaIMP, blaSPM, blaVIM, vanA, vanB and mecA were screened by SYBR Green reactions. Therapeutic efficacy was evaluated at the time of positive blood culture and at final phenotypic identification and antimicrobial susceptibility results. We analyzed 69 episodes of BSI from 64 patients. Gram-positive bacteria were identified in 61 % of the samples, Gram-negative bacteria in 32 % and fungi in 7 %. There was 78.2 % of agreement between the phenotypic and molecular methods in final species identification. The mecA gene was detected in 81.4 % of Staphylococcus spp., and 91.6 % were concordant with the phenotypic method. Detection of vanA gene was 100 % concordant. The concordance for Gram-negative susceptibilities was 71.4 % for Enterobacteriaceae and 50 % for Pseudomonas aeruginosa. Therapy was more frequently inadequate in patients who died, and the molecular test was concordant with the phenotypic susceptibility test in 50 %. qPCR has potential indication for early identification of pathogens and antimicrobial resistance genes from BSI in paediatric cancer patients and may improve antimicrobial therapy.

  14. Prevalence of Diverse Clones of Vancomycin-Resistant Enterococcus faecium ST78 in a Chinese Hospital.

    Science.gov (United States)

    Yang, Jiyong; Jiang, Yufeng; Guo, Ling; Ye, LIyan; Ma, Yanning; Luo, Yanping

    2016-06-01

    Vancomycin-resistant Enterococcus (VRE) has been identified in China. However, little is known about the spread of VRE isolates. The genetic relatedness of vancomycin-resistant Enterococcus faecium (VREfm) isolates was analyzed by pulsed-field gel electrophoresis (PFGE), their antimicrobial susceptibilities were analyzed by E-test and the VITEK 2 AST-GP67 test Kit, and their sequence types (STs) were investigated by multilocus sequence typing (MLST). S1-PFGE was used for plasmid profiling, and PCR and subsequent sequencing were performed to identify the virulence genes. A total of 96 nonduplicated VREfm isolates were obtained and categorized into 38 PFGE types (type 1-38). The predominant MLST type was ST78, while ST17, ST341, and ST342 were also sporadically identified. All types of clinical VREfm strains harbored the vanA gene; however, they carried plasmids of different sizes. While 92.1%, 71.1%, and 60.5% of VREfm strains carried hyl, scm, and ecbA genes, respectively, all of them were positive for esp, acm, sgrA, pilA, and pilB genes. Clonal VREfm spread was observed, and nonplasmid-mediated horizontal transfer of vancomycin-resistant gene might have conveyed resistance to some vancomycin-susceptible E. faecium strains. E. faecium ST78 carrying vanA gene was the most prevalent clone in this study. The high prevalence of virulence genes, including esp, hyl, acm, scm, ecbA, sgrA, pilA, and pilB, confirmed their important roles in the emergence of VREfm ST78 in nosocomial infections.

  15. Complex Routes of Nosocomial Vancomycin-Resistant Enterococcus faecium Transmission Revealed by Genome Sequencing.

    Science.gov (United States)

    Raven, Kathy E; Gouliouris, Theodore; Brodrick, Hayley; Coll, Francesc; Brown, Nicholas M; Reynolds, Rosy; Reuter, Sandra; Török, M Estée; Parkhill, Julian; Peacock, Sharon J

    2017-04-01

    Vancomycin-resistant Enterococcus faecium (VREfm) is a leading cause of nosocomial infection. Here, we describe the utility of whole-genome sequencing in defining nosocomial VREfm transmission. A retrospective study at a single hospital in the United Kingdom identified 342 patients with E. faecium bloodstream infection over 7 years. Of these, 293 patients had a stored isolate and formed the basis for the study. The first stored isolate from each case was sequenced (200 VREfm [197 vanA, 2 vanB, and 1 isolate containing both vanA and vanB], 93 vancomycin-susceptible E. faecium) and epidemiological data were collected. Genomes were also available for E. faecium associated with bloodstream infections in 15 patients in neighboring hospitals, and 456 patients across the United Kingdom and Ireland. The majority of infections in the 293 patients were hospital-acquired (n = 249) or healthcare-associated (n = 42). Phylogenetic analysis showed that 291 of 293 isolates resided in a hospital-associated clade that contained numerous discrete clusters of closely related isolates, indicative of multiple introductions into the hospital followed by clonal expansion associated with transmission. Fine-scale analysis of 6 exemplar phylogenetic clusters containing isolates from 93 patients (32%) identified complex transmission routes that spanned numerous wards and years, extending beyond the detection of conventional infection control. These contained both vancomycin-resistant and -susceptible isolates. We also identified closely related isolates from patients at Cambridge University Hospitals NHS Foundation Trust and regional and national hospitals, suggesting interhospital transmission. These findings provide important insights for infection control practice and signpost areas for interventions. We conclude that sequencing represents a powerful tool for the enhanced surveillance and control of nosocomial E. faecium transmission and infection.

  16. Antibiotic Susceptibility Pattern of Gram-positive Cocci Cultured from Patients in Three University Hospitals in Tehran, Iran during 2001-2005

    Directory of Open Access Journals (Sweden)

    Aligholi Marzieh

    2009-10-01

    Full Text Available Bacterial resistance to antibiotics is a serious problem and is increasing in prevalence world-wide at an alarming rate. The antimicrobial susceptibility patterns of 1897 gram-positive bacterial Isolates were evaluated. The minimum inhibitory concentration (MIC of isolates which comprised Staphylococcus aureus (927 isolates, coagulase-negative staphylococci (CNS; 425 isolates, Enterococcus faecalis (320 isolates, Enterococcus faecium (157 isolates, and pneumococci (50 isolates collected from 3 teaching hospitals in Tehran were determined by agar dilution method according to Clinical and Laboratory Standards Institute (CLSI guidelines. The presence of mecA gene was investigated in methicillin-resistant staphylococci by PCR method and vanA and vanB genes were targeted in enterococcal isolates by Multiplex PCR method. The resistance rate to methicillin among S. aureus and CNS isolates were 33% and 49%, respectively. All S. aureus isolates were susceptible to vancomycin .The lowest rate of resistance in all S. aureus isolates was found for rifampicin (<4%. The vancomycin resistance rate in enterococci isolates was 11% which was more frequent among E. faecium (19% than E. faecalis (4%, all resistant isolates carrying vanA. High-level resistance to gentamicin and streptomycin, were detected in 47% and 87% of enterococcal isolates respectively. The rate of penicillin resistance in pneumococci was 3% and about 27% of isolates had reduced susceptibility to penicillin. The prevalence of erythromycin resistant among pneumococci was 58%. All pneumococcal isolates were susceptible to ceftriaxone, rifampicin and vancomycin. Our data highlight the importance of access to updated bacterial susceptibility data regarding commonly prescribed agents for clinicians in Iran.

  17. Comparison of PCR/Electron spray Ionization-Time-of-Flight-Mass Spectrometry versus Traditional Clinical Microbiology for active surveillance of organisms contaminating high-use surfaces in a burn intensive care unit, an orthopedic ward and healthcare workers

    Directory of Open Access Journals (Sweden)

    Yun Heather C

    2012-10-01

    Full Text Available Abstract Background Understanding nosocomial pathogen transmission is restricted by culture limitations. Novel platforms, such as PCR-based electron spray ionization-time-of-flight-mass spectrometry (ESI-TOF-MS, may be useful as investigational tools. Methods Traditional clinical microbiology (TCM and PCR/ESI-TOF-MS were used to recover and detect microorganisms from the hands and personal protective equipment of 10 burn intensive care unit (ICU healthcare workers providing clinical care at a tertiary care military referral hospital. High-use environmental surfaces were assessed in 9 burn ICU and 10 orthopedic patient rooms. Clinical cultures during the study period were reviewed for pathogen comparison with investigational molecular diagnostic methods. Results From 158 samples, 142 organisms were identified by TCM and 718 by PCR/ESI-TOF-MS. The molecular diagnostic method detected more organisms (4.5 ± 2.1 vs. 0.9 ± 0.8, p S. aureus in 13 samples vs. 21 by PCR/ESI-TOF-MS. Gram-negative organisms were less commonly identified than gram-positive by both methods; especially by TCM. Among all detected bacterial species, similar percentages were typical nosocomial pathogens (18-19% for TCM vs. PCR/ESI-TOF-MS. PCR/ESI-TOF-MS also detected mecA in 112 samples, vanA in 13, and KPC-3 in 2. MecA was associated (p S. aureus. No vanA was codetected with enterococci; one KPC-3 was detected without Klebsiella spp. Conclusions In this pilot study, PCR/ESI-TOF-MS detected more organisms, especially gram-negatives, compared to TCM, but the current assay format is limited by the number of antibiotic resistance determinants it covers. Further large-scale assessments of PCR/ESI-TOF-MS for hospital surveillance are warranted.

  18. Absence of intestinal colonization by vancomycin-resistant enterococci in nonhuman primates Ausência de enterococos resistentes à vancomicina na microbiota intestinal de primatas não-humanos

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    Diego Batista Xavier

    2010-06-01

    Full Text Available The animal reservoirs of vancomycin-resistant enterococci (VRE have important role in the epidemiology of the bacteria and resistant genes. The present work searched fecal samples taken off nonhuman primates for the presence of VRE. Resistance profiles, virulence traits, and genetic variability among enterococci isolates were also analyzed. The samples included Capuchin monkeys (Cebus apella, n=28 and Common marmoset (Callithrix penicillata, n=37 housed in the Primate Center of the University of Brasília, Brazil. Most individuals were captive monkeys from the Central-West and South-East regions of Brazil (n=48. We collected rectal swabs and carried out selective isolation followed by multiplex Polymerase Chain Reaction (PCR to identify species and resistance genes. No vanA or vanB-containing enterococci were found. The carriage rates ranged from 1.5% for the VanC-type E. casseliflavus and E. gallinarum until 12.3% (n=8 for Enterococcus faecalis. All E. faecalis isolates showed susceptibility to vancomycin, teicoplanin, ampicillin, gentamicin, and streptomycin. The virulence genes ace and esp were prevalent (100.0%, 87.5%. Multilocus variable number of tandem repeats (MLVA revealed diversity in the number of repeats among E. faecalis isolates and targets, which was higher for espC, efa5, and efa6. We identified six different MLVA genotypes that were divergent from those described in human beings. Also, they were clustered into two genogroups that showed host-specificity for the species Cebus apella or Callithrix penicillata. In conclusion, no vanA- or vanB-containing enterococci were found colonizing those primate individuals. This finding suggested that the primate individuals investigated in our study are not directly involved in the epidemiological chain of high-level vancomycin-resistant genes vanA or vanB in Brazil. Our study also showed that E. faecalis isolated from nonhuman primates carry virulence traits and have ability to spread their

  19. Development of a heptaplex PCR assay for identification of Staphylococcus aureus and CoNS with simultaneous detection of virulence and antibiotic resistance genes.

    Science.gov (United States)

    Okolie, Charles Emeka; Wooldridge, Karl G; Turner, David P J; Cockayne, Alan; James, Richard

    2015-08-05

    Staphylococcal toxicity and antibiotic resistance (STAAR) have been menacing public health. Although vancomycin-resistant Staphylococcus aureus (VRSA) is currently not as widespread as methicillin-resistant S. aureus (MRSA), genome evolution of MRSA into VRSA, including strains engineered within the same patient under anti-staphylococcal therapy, may build up to future public health concern. To further complicate diagnosis, infection control and anti-microbial chemotherapy, non-sterile sites such as the nares and the skin could contain both S. aureus and coagulase-negative staphylococci (CoNS), either of which could harbour mecA the gene driving staphylococcal methicillin-resistance and required for MRSA-VRSA evolution. A new heptaplex PCR assay has been developed which simultaneously detects seven markers for: i) eubacteria (16S rRNA), ii) Staphylococcus genus (tuf), iii) Staphylococcus aureus (spa), iv) CoNS (cns), v) Panton-Valentine leukocidin (pvl), vi) methicillin resistance (mecA), and vii) vancomycin resistance (vanA). Following successful validation using 255 reference bacterial strains, applicability to analyse clinical samples was evaluated by direct amplification in spiked blood cultures (n = 89) which returned 100 % specificity, negative and positive predictive values. The new assay has LoD of 1.0x10(3) CFU/mL for the 16S rRNA marker and 1.0x10(4) CFU/mL for six other markers and completes cycling in less than one hour. The speed, sensitivity (100 %), NPV (100 %) and PPV (100 %) suggest the new heptaplex PCR assay could be easily integrated into a routine diagnostic microbiology workflow. Detection of the cns marker allows for unique identification of CoNS in mono-microbial and in poly-microbial samples containing mixtures of CoNS and S. aureus without recourse to the conventional elimination approach which is ambiguous. In addition to the SA-CoNS differential diagnostic essence of the new assay, inclusion of vanA primers will allow

  20. Análisis clínico-epidemiológico de la portación intestinal de enterococos resistentes a vancomicina en una unidad de terapia intensiva Clinical and epidemiologic analysis of intestinal tract colonization with vancomycin-resistant enterococci in an intensive care unit

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    A.M. Togneri

    2005-03-01

    Full Text Available En un período de cinco meses y 25 días se investigó la portación intestinal de enterococos resistentes a vancomicina (EVR. Se estudiaron 124 pacientes (73% de 171 admitidos en la unidad de terapia intensiva (UTI, 35 de los cuales (28% resultaron ser portadores. Los aislamientos de EVR (n=35 fueron identificados como Enterococcus faecium (n=18, Enterococcus gallinarum (n=16 y Enterococcus raffinosus (n=1. Todos los aislamientos estudiados fueron resistentes a vancomicina (VAN (CIM90= 512 µg/ml y teicoplanina (CIM90= 32 µg/ml y portaban el gen vanA. Los estudios de tipificación molecular mostraron un alto grado de homología entre los aislamientos de E. faecium (un clon dominante y E. gallinarum (dos tipos clonales, sugiriendo su diseminación a modo de brote. No se encontraron diferencias significativas con la edad y el sexo de los pacientes no portadores (p>0,05, pero si con el tiempo de hospitalización y el uso de esquemas antibióticos de amplio espectro (pIntestinal tract colonization with vancomycin resistant enterococci (VRE was studied during five months and 25 days. Out of 171 patients hospitalized in the intensive care unit, 124 (73% were included in this study. Thirty five of them (28% were recognized as colonized with VRE. VRE isolates (n = 35 were identified as Enterococcus faecium (n=18, Enterococcus gallinarum (n=16, and Enterococcus raffinosus (n=1. All of them were resistant to vancomycin (MIC90= 512 µg/ml and to teicoplanin (MIC90= 32 µg/ml, having the vanA gene. By means of molecular methods a high homology was found among E. faecium and E. gallinarum isolates, respectively, suggesting their spread as a kind of outbreak. No significant differences in age or sex were found among colonized and non-colonized patients (p>0.05. On the other hand, the hospitalization time and the use of broad-spectrum antibiotics were associated with colonization. From this study we highlight the importance of enhancing all measures of

  1. Antibiotic resistance of faecal enterococci in poultry, poultry farmers and poultry slaughterers.

    Science.gov (United States)

    van den Bogaard, A E; Willems, R; London, N; Top, J; Stobberingh, E E

    2002-03-01

    The prevalence of resistance in enterococci to antibiotics, commonly used for therapy in poultry or as antimicrobial growth promoters (AMGPs), was determined in faecal samples of two chicken populations: broilers in which antibiotic and AMGP use is common and laying-hens with a low antibiotic usage. In addition faecal samples were examined from three human populations: broiler farmers, laying-hen farmers and poultry slaughterers. MICs of an extended panel of antibiotics for a randomly chosen gentamicin- or vancomycin-resistant enterococcal isolate from each faecal specimen were also determined. The prevalence of resistance for all antibiotics tested was higher in broilers than in laying-hens. Resistance in faecal enterococci of broiler farmers was for nearly all antibiotics higher than those observed in laying-hen farmers and poultry slaughterers. The overall resistance in broilers was correlated with the resistance in broiler farmers and in poultry slaughterers. No correlation between the results obtained in the laying-hens with any of the other populations was found. The 27 gentamicin-resistant isolates all showed high-level resistance to gentamicin and two of these isolates, both Enterococcus faecium, were resistant to all antibiotics tested, except vancomycin. The 73 vancomycin-resistant enterococci (VRE) isolated from the five populations belonged to four different species and in all isolates the vanA gene cluster was detected by blot hybridization. The pulsed-field gel electrophoresis (PFGE) patterns of these vancomycin-resistant enterococci were quite heterogeneous, but Enterococcus hirae isolates with the same or a closely related PFGE pattern were isolated at two farms from the broiler farmer and from broilers. Molecular characterization of vanA-containing transposons of these isolates showed that similar transposon types, predominantly found in poultry, were present. Moreover, similar vanA elements were not only found in isolates with the same PFGE

  2. Comparison of PCR/Electron spray Ionization-Time-of-Flight-Mass Spectrometry versus Traditional Clinical Microbiology for active surveillance of organisms contaminating high-use surfaces in a burn intensive care unit, an orthopedic ward and healthcare workers

    Science.gov (United States)

    2012-01-01

    Background Understanding nosocomial pathogen transmission is restricted by culture limitations. Novel platforms, such as PCR-based electron spray ionization-time-of-flight-mass spectrometry (ESI-TOF-MS), may be useful as investigational tools. Methods Traditional clinical microbiology (TCM) and PCR/ESI-TOF-MS were used to recover and detect microorganisms from the hands and personal protective equipment of 10 burn intensive care unit (ICU) healthcare workers providing clinical care at a tertiary care military referral hospital. High-use environmental surfaces were assessed in 9 burn ICU and 10 orthopedic patient rooms. Clinical cultures during the study period were reviewed for pathogen comparison with investigational molecular diagnostic methods. Results From 158 samples, 142 organisms were identified by TCM and 718 by PCR/ESI-TOF-MS. The molecular diagnostic method detected more organisms (4.5 ± 2.1 vs. 0.9 ± 0.8, p < 0.01) from 99% vs. 67% of samples (p < 0.01). TCM detected S. aureus in 13 samples vs. 21 by PCR/ESI-TOF-MS. Gram-negative organisms were less commonly identified than gram-positive by both methods; especially by TCM. Among all detected bacterial species, similar percentages were typical nosocomial pathogens (18-19%) for TCM vs. PCR/ESI-TOF-MS. PCR/ESI-TOF-MS also detected mecA in 112 samples, vanA in 13, and KPC-3 in 2. MecA was associated (p < 0.01) with codetection of coagulase negative staphylococci but not S. aureus. No vanA was codetected with enterococci; one KPC-3 was detected without Klebsiella spp. Conclusions In this pilot study, PCR/ESI-TOF-MS detected more organisms, especially gram-negatives, compared to TCM, but the current assay format is limited by the number of antibiotic resistance determinants it covers. Further large-scale assessments of PCR/ESI-TOF-MS for hospital surveillance are warranted. PMID:23050585

  3. ANTIBIOTIC RESISTANCE IN LACTIC ACID BACTERIA ISOLATED FROM FERMENTED DAIRY PRODUCTS AND BOZA

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    Gamze Başbülbül

    2015-06-01

    Full Text Available In this study, the resistance of 83 strains of lactic acid bacteria isolated from Turkish cheese, yogurt, kefir and boza samples to 6 antibiotics (gentamicin, tetracycline, chloramphenicol, erythromycin, vancomycin and ciprofloxacin was evaluated. The 83 isolates were identified by 16S rRNA gene sequencing and according to BLAST comparisons with sequences in the data banks, those strains showing the highest similarities with the isolates were Enterococcus faecium (10, Lactococcus lactis subsp. Lactis (10, Lactobacillus fermentum (6, Lactobacillus plantarum (6, Lactobacillus coryniformis (7, Lactobacillus casei (13, Leuconostoc mesenteroides (14, Pediococcus pentosaceus (10, Weisella confusa (7. Antimicrobial resistance of strains to 6 antibiotics was determined using the agar dilution method. The antibiotic resistance among all the isolates was detected against chloramphenicol (31,3 % of the isolates, tetracycline (30,1 %, erythromycin (2,4 %, ciprofloxacin (2,41%, vancomycin (73,5 %, intrinsic resistance. Overall 19,3 % of the isolates showed resistance against multiple antibiotics. Antibiotic resistance genes were studied by PCR and the following genes were detected; tet(M gene in Lactobacillus fermentum (1, Lactobacillus plantarum (1, Pediococcus pentosaceus (5, Enterococcus faecium (2, Weisella confusa (4 and the vancomycin resistance gene van(A in one Weisella confusa strain.

  4. Streptomyces polyantibioticus sp. nov., isolated from the banks of a river.

    Science.gov (United States)

    le Roes-Hill, Marilize; Meyers, Paul R

    2009-06-01

    As part of an antibiotic-screening programme, an actinomycete, designated strain SPR(T), was isolated from soil collected from the banks of the Umgeni River, KwaZulu-Natal Province, South Africa. The isolate produced branching vegetative mycelia with sporangiophores bearing sporangia developing at a late stage of growth. The sporangia contained smooth, almond-shaped, non-motile spores. Strain SPR(T) exhibited antibiosis against various Gram-positive and Gram-negative bacteria, including Enterococcus faecium VanA (a vancomycin-resistant strain), Mycobacterium aurum A+ and Escherichia coli ATCC 25922. The chemotaxonomic characteristics of the strain, with the exception of the phospholipid pattern, corresponded with those of the members of the family Streptomycetaceae Waksman and Henrici 1943. Furthermore, phylogenetic analysis based on 16S rRNA genes showed that the strain was closely related to members of the genus Streptomyces, which supports its classification in the family Streptomycetaceae. Thus strain SPR(T) represents a novel species of the genus Streptomyces, for which the name Streptomyces polyantibioticus sp. nov. is proposed. The type strain is SPR(T) (=DSM 44925(T)=NRRL B-24448(T)).

  5. Evaluation of Verigene Blood Culture Test Systems for Rapid Identification of Positive Blood Cultures

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    Jae-Seok Kim

    2016-01-01

    Full Text Available The performance of molecular tests using the Verigene Gram-Positive and Gram-Negative Blood Culture nucleic acid tests (BC-GP and BC-GN, resp.; Naosphere, Northbrook, IL, USA was evaluated for the identification of microorganisms detected from blood cultures. Ninety-nine blood cultures containing Gram-positive bacteria and 150 containing Gram-negative bacteria were analyzed using the BC-GP and BC-GN assays, respectively. Blood cultures were performed using the Bactec blood culture system (BD Diagnostic Systems, Franklin Lakes, NJ, USA and conventional identification and antibiotic-susceptibility tests were performed using a MicroScan system (Siemens, West Sacramento, CA, USA. When a single strain of bacteria was isolated from the blood culture, Verigene assays correctly identified 97.9% (94/96 of Gram-positive bacteria and 93.8% (137/146 of Gram-negative bacteria. Resistance genes mecA and vanA were correctly detected by the BC-GP assay, while the extended-spectrum β-lactamase CTX-M and the carbapenemase OXA resistance gene were detected from 30 cases cultures by the BC-GN assay. The BC-GP and BC-GN assays showed high agreement with conventional identification and susceptibility tests. These tests are useful for rapid identification of microorganisms and the detection of clinically important resistance genes from positive Bactec blood cultures.

  6. Reductions of bacterial antibiotic resistance through five biological treatment processes treated municipal wastewater.

    Science.gov (United States)

    Yuan, Qing-Bin; Guo, Mei-Ting; Wei, Wu-Ji; Yang, Jian

    2016-10-01

    Wastewater treatment plants are hot spots for antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs). However, limited studies have been conducted to compare the reductions of ARB and ARGs by various biological treatment processes. The study explored the reductions of heterotrophic bacteria resistant to six groups of antibiotics (vancomycin, gentamicin, erythromycin, cephalexin, tetracycline, and sulfadiazine) and corresponding resistance genes (vanA, aacC1, ereA, ampC, tetA, and sulI) by five bench-scale biological reactors. Results demonstrated that membrane bioreactor (MBR) and sequencing batch reactor (SBR) significantly reduced ARB abundances in the ranges of 2.80∼3.54 log and 2.70∼3.13 log, respectively, followed by activated sludge (AS). Biological filter (BF) and anaerobic (upflow anaerobic sludge blanket, UASB) techniques led to relatively low reductions. In contrast, ARGs were not equally reduced as ARB. AS and SBR also showed significant potentials on ARGs reduction, whilst MBR and UASB could not reduce ARGs effectively. Redundancy analysis implied that the purification of wastewater quality parameters (COD, NH4 (+)-N, and turbidity) performed a positive correlation to ARB and ARGs reductions.

  7. El teatro Español en Ljubljana entre 1945-1990

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    Rastko Rafael Djordjević

    1991-12-01

    Full Text Available Una mañana pasada en el archivo del Thatro Nacional de Ljubljana resulta más que suficiente para poder constatar que las obras teatrales españolas han sido estrenadas raramente durante los últimos cuarenta años y esto con mucha irregularidad. Los años cincuenta (el período después de la Segunda Guerra Mundial se mostraron favorables al teatro español; el público de Ljubljana encuentra en las piezas de Lorca y en las del Siglo de Oro algo diferente. Los espectadores viven el destino trágico, la espera vana, el camino irrealizado, Ia muerte con Ia que concluye el anhelo de vivir de los héroes de Lorca; los autores del Siglo de Oro con sus temas caballerescos de honor y de venganza y con su sentido del humor sorprenden y entusiasman. De este modo, se estrenan desde 1949 hasta 1959 tres obras maestras de Lorca:1950 La casa de BemardaAlba,1955 Bodas de sangre y finalmente en 1959 Yerma.

  8. Mapas en escenarios de cambio: el caso de la provincia de Río Negro, Argentina

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    Paula Gabriela Núñez

    2015-01-01

    Full Text Available En este trabajo se analiza la forma en que se fue graficando la provincia de Río Negro en Argentina,desde su formación en 1958 hasta el golpe de Estado de 1976. Se contextualizan los esquemasterritoriales y mapas de la provincia en los modos precedentes de graficar el territorio patagónico,indagando en las diversas tensiones y dinámicas que incidieron en su formación como provincia,y las rupturas y continuidades presentes en el modo de caracterizar el espacio. Estos elementospermitirán evidenciar el carácter coyuntural de las representaciones gráficas del territorio.También muestran la pretensión de objetividad y neutralidad de los mismos, dando cuenta dela comprensión espacial como parte de una planificación política antes que de una observacióndirecta, que se observa en las diferentes formas de dibujar la superficie afectada a la jurisdicciónprovincial.Como resultado encontramos que los sucesivos gobiernos provinciales, entre 1958 y 1976, vana fundamentar una política distributiva desigual usando un discurso geográfico que justificó elestablecimiento de marginalidades internas.

  9. Elva, Viljandi,Tallinna ja Kuremäe võistlused / Karin Bachmann, Peeter Pere, Endrik Mänd, Veljo Kaasik

    Index Scriptorium Estoniae

    2016-01-01

    Eesti Vabariik 100 võistlussarja raames korraldatud arhitektuurivõistlustest Elvas, Viljandis, Tallinnas ja Kuremäel. Elva linn valis võistlusobjektiks kaubanduskeskuse ja parkla laienduse. Esimese preemia sai võistlustöö "Sõstrapõõsaste vahel" (autorid: Ülle Maiste, Diana Taalfeld, Anne Saarniit, Roomet Helbre ja Taavi Kuningas). Viljandi linna võistluse eesmärk oli leida terviklik ruumiline lahendus Vabaduse platsi ja selle lähialade ning Tallinna, Vaksali ja Vana-Vaksali tänava arenduseks. Esimese preemia sai võistlustöö "Tasa sõuab" (autorid: Ülle Maiste, Diana Taalfeld, Anne Saarniit, Roomet Helbre, Taavi Kuningas ja Mihkel Urmet). Tallinna linn valis võistlusobjektiks peatänava. Esimese preemia sai võistlustöö "Kevad linnas" (autorid: Toomas Paaver, Indrek Kustavus, Indrek Peil, Siiri Vallner, Kristel Niisuke, Valdis Linde ja Riin-Kärt Ranne). Kuremäe küla valis võistlusobjektiks küla keskuse kujundamise koos uute funktsioonide lisamisega. Esimese preemia sai võistlustöö "Rännak" (autorid: Helen Rebane, Kaie Kuldkepp ja Pelle-Sten Viiburg)

  10. Lastekirjandus vallutab uusi tippe : eesti lastekirjandus 2009 / Jaanus Vaiksoo

    Index Scriptorium Estoniae

    Vaiksoo, Jaanus, 1967-

    2010-01-01

    Ülevaade 2009. aasta eesti lastekirjandusest. Pikemalt käsitletakse järgmisi autoreid ja nende teoseid: Kadi Hinrikus "Kui emad olid väikesed", Kristiina Kass "Peeter ja mina", Mika Keränen "Varastatud oranž jalgratas", Andrus Kivirähk "Kaka ja kevad", Aino Pervik "Ühes väikses veidras linnas", Maarja Undusk "Päkapikk Ingo", Ilmar Tomusk "Vend Johannes", Indrek Koff "Enne kooli", Piret Raud "Härra Linnu lugu", Wimberg "Suur pidusöök", Artur Jurin "Puru kuningriigi lood II", Joonas Sildre "Maailma naba", kogumik "Uued eesti muinasjutud", Aino Pervik "Tirilinna lood", Aimeé Beekman "Päästekoer Walter", Eve Ernis "Karupoeg Nummi seiklused", Priit Aimla "Torisev vanatoi", Juhani Püttsepp "Väikese hundi lood" ja "Lauajupi Madonna", Epp Petrone "Siis, kui seened veel rääkisid", Heiki Vilep "Habemega nali", "Nõiutud linn", Leelo Tungal "Naljatilgad lähevad laulupeole", "Kama üks ja kama kaks", koos Peep Pedmansoniga Miriami lood"(esimene 3D-piltidega lasteraamat), Aidi Vallik "Pints ja Tutsik" 2. osa, Mare Müürsepp "Viis vaba kutsikat", Tiia Selli "Kass Kriibik", Aleksei Turovski "Kassipoeg Võilill", Kaider Vardja "Vana maja lugu", Merca "Mullivesi", Ülle Kütsen "Väike puu ja rändur kuu", Lehte Hainsalu "Mängiks midagi", Peep Veedla "Pöialpoiss"

  11. [Antimicrobial spectrum of dalbavancin. Mechanism of action and in vitro activity against Gram-positive microorganisms].

    Science.gov (United States)

    Cercenado, Emilia

    2017-01-01

    Because of the increase in bacterial resistance, there is a need for new antimicrobial agents. Dalbavancin is a semisynthetic glycopeptide that inhibits the late stages of bacterial cell wall synthesis in the same way as vancomycin, but in addition, its lipophilic side chain anchors dalbavancin to the cellular membrane and allows enhanced activity compared with that of vancomycin. Dalbavancin possesses a broad spectrum of in vitro activity against Gram-positive aerobic and anaerobic microorganisms, being 4-8 times more potent than vancomycin. The spectrum of dalbavancin includes staphylococci, enterococci, streptococci, and anaerobic Gram-positive cocci and bacilli. It is active against different species of multiresistant microorganisms, including methicillin-resistant Staphylococcus aureus and penicillin-resistant viridans streptococci and Streptococcus pneumoniae. Although it shows in vitro activity against Enterococcus spp., it is inactive against isolates expressing the VanA phenotype of vancomycin resistance. It also shows slow bactericidal activity against S. aureus, coagulase-negative staphylococci, and Streptococcus pyogenes. In general, the MIC90 (minimum inhibitory concentration 90%) against the majority of the microorganisms is 0.06mg/L and, more than 98% of the isolates that have been tested are inhibited at concentrations of ≤ 0.12mg/L. Dalbavancin is an interesting addition to the therapeutic armamentarium for the treatment of infections caused by Gram-positive microorganisms, including multidrug-resistant isolates. Copyright © 2017 Elsevier España, S.L.U. All rights reserved.

  12. Getting insight into the prevalence of antibiotic resistance genes in specimens of marketed edible insects.

    Science.gov (United States)

    Milanović, Vesna; Osimani, Andrea; Pasquini, Marina; Aquilanti, Lucia; Garofalo, Cristiana; Taccari, Manuela; Cardinali, Federica; Riolo, Paola; Clementi, Francesca

    2016-06-16

    This study was aimed at investigating the occurrence of 11 transferable antibiotic resistance (AR) genes [erm(A), erm(B), erm(C), vanA, vanB, tet(M), tet(O), tet(S), tet(K), mecA, blaZ] in 11 species of marketed edible insects (small crickets powder, small crickets, locusts, mealworm larvae, giant waterbugs, black ants, winged termite alates, rhino beetles, mole crickets, silkworm pupae, and black scorpions) in order to provide a first baseline for risk assessment. Among the AR genes under study, tet(K) occurred with the highest frequency, followed by erm(B), tet(S) and blaZ. A high variability was seen among the samples, in terms of occurrence of different AR determinants. Cluster Analysis and Principal Coordinates Analysis allowed the 11 samples to be grouped in two main clusters, one including all but one samples produced in Thailand and the other including those produced in the Netherlands. Copyright © 2016 Elsevier B.V. All rights reserved.

  13. First outbreak of colonization by linezolid- and glycopeptide-resistant Enterococcus faecium harbouring the cfr gene in a UK nephrology unit.

    Science.gov (United States)

    Inkster, T; Coia, J; Meunier, D; Doumith, M; Martin, K; Pike, R; Imrie, L; Kane, H; Hay, M; Wiuff, C; Wilson, J; Deighan, C; Hopkins, K L; Woodford, N; Hill, R

    2017-07-08

    To describe an outbreak of colonization by linezolid- and glycopeptide-resistant Enterococcus faecium harbouring the cfr gene in a UK nephrology unit. Isolates of linezolid-resistant E. faecium were typed by pulsed-field gel electrophoresis (PFGE), and examined by polymerase chain reaction (PCR) and sequencing for the transmissible cfr gene that confers resistance to linezolid. Enhanced environmental cleaning, initial and weekly screening of all patients, and monitoring of adherence to standard infection control precautions were implemented. Five patients with pre-existing renal disease were found to have rectal colonization with linezolid-resistant E. faecium over a two-week period. The index case was a 57-year-old male from India who had travelled to the UK. One patient also had a linezolid-resistant E. faecium of a different PFGE profile isolated from a heel wound. All isolates were confirmed to harbour the cfr gene by PCR and Sanger sequencing, and all were resistant to glycopeptides (VanA phenotype). This article describes the first UK outbreak with a single strain of linezolid- and glycopeptide-resistant E. faecium harbouring the cfr gene, affecting five patients in a nephrology unit. Following the implementation of aggressive infection control measures, no further cases were detected beyond a two-week period. Crown Copyright © 2017. Published by Elsevier Ltd. All rights reserved.

  14. Genetic characterisation of antibiotic resistance and virulence factors in vanA-containing enterococci from cattle, sheep and pigs subsequent to the discontinuation of the use of avoparcin.

    Science.gov (United States)

    Ramos, Sónia; Igrejas, Gilberto; Rodrigues, Jorge; Capelo-Martinez, José-Luis; Poeta, Patrícia

    2012-07-01

    The prevalence of vancomycin resistant-enterococci (VRE) in faecal samples from cattle, sheep and pigs slaughtered for human consumption was evaluated. Enterococci containing the vanA gene were detected in 25.3% and 2.7% of the porcine and ovine samples, respectively, and were identified as Enterococcus faecium. No vanA-containing enterococcal strains were detected in bovine samples. Enterococcal strains with intrinsic vancomycin resistance were detected in seven (9.9%) faecal samples from pigs and in two samples from both cattle and sheep (3.7% and 2.7%, respectively). All vanA-positive isolates from pigs were resistant to tetracycline and erythromycin, and the mobile element Tn916/Tn1545-like transposon was detected in 90.5% of the tetracycline-resistant isolates that contained the tet(M) gene. Although gelatinase and haemolytic activity were not detected, the hyl and cylB virulence genes were found within the VRE strains isolated. Copyright © 2011 Elsevier Ltd. All rights reserved.

  15. Leuconostoc mesenteroides SJRP55: A Bacteriocinogenic Strain Isolated from Brazilian Water Buffalo Mozzarella Cheese.

    Science.gov (United States)

    de Paula, Aline Teodoro; Jeronymo-Ceneviva, Ana Beatriz; Silva, Luana Faria; Todorov, Svetoslav Dimitrov; Franco, Bernadette Dora Gombossy de Melo; Choiset, Yvan; Haertlé, Thomas; Chobert, Jean-Marc; Dousset, Xavier; Penna, Ana Lúcia Barretto

    2014-12-01

    The production of bacteriocins by Leuconostoc mesenteroides represents an important opportunity for exploration of their potential use for industrial purpose. The antimicrobial compounds produced by L. mesenteroides subsp. mesenteroides SJRP55 strain were characterized and purified. Cell-free supernatant of Leuc. mesenteroides subsp. mesenteroides SJRP55 produced antibacterial compounds against Listeria spp. strains and not inhibiting against Lactobacillus spp. The antimicrobial substances were stable at high temperatures (100 °C for 2 h and 121 °C for 20 min) and low pH (pH 2-4) values, but sensitive to proteolytic enzymes and resistant to α-amylase, lipase and catalase enzymes. The optimal temperature for active peptides production was 25 °C. The antimicrobial compounds were purified by ammonium sulfate precipitation, affinity column and reverse-phase chromatography. Mass spectrometry and amino acids analyses showed that the bacteriocins were identical to mesentericin Y105 and B105. The producer strain's DNA analysis revealed presence of open reading frames possibly coding for virulence factors, such as enterococcal surface protein (esp), collagen adhesion (ace) and intrinsic vancomycin resistance (vanA); however, biogenic amines encoding genes were not observed. Leuc. mesenteroides subsp. mesenteroides SJRP55 is a promising biopreservative culture in fermented milk, and the purified bacteriocins can also be applied in food preservation.

  16. Bacteriocinogenic potential and safety evaluation of non-starter Enterococcus faecium strains isolated from home made white brine cheese.

    Science.gov (United States)

    Favaro, Lorenzo; Basaglia, Marina; Casella, Sergio; Hue, Isabelle; Dousset, Xavier; Gombossy de Melo Franco, Bernadette Dora; Todorov, Svetoslav Dimitrov

    2014-04-01

    Four LAB strains, isolated from Bulgarian home made white brine cheese, were selected for their effective inhibition against Listeria monocytogenes. According to their biochemical and physiological characteristics, the strains were classified as members of Enterococcus genus, and then identified as Enterococcus faecium by 16S rDNA sequencing. Their bacteriocin production and inhibitory spectrum were evaluated together with the occurrence of several bacteriocin genes (entA, entB, entP, entL50B). Their virulence potential and safety was assessed both using PCR targeted to the genes gelE, hyl, asa1, esp, cylA, efaA, ace, vanA, vanB, hdc1, hdc2, tdc and odc and by phenotypical tests for antibiotic resistance, gelatinase, lipase, DNAse and α- and β-haemolysis. The E. faecium strains harboured at least one enterocin gene while the occurrence of virulence, antibiotic resistance and biogenic amines genes was limited. Considering their strong antimicrobial activity against L. monocytogenes strains, the four E. faecium strains exhibited promising potential as bio-preservatives cultures for fermented food productions. Copyright © 2013 Elsevier Ltd. All rights reserved.

  17. RNA isolation for small RNA Next-Generation Sequencing from acellular biofluids.

    Science.gov (United States)

    Burgos, Kasandra L; Van Keuren-Jensen, Kendall

    2014-01-01

    There are a number of considerations when choosing protocols both upstream and downstream of Next-Generation Sequencing experiments. On the front end, purification methods, additives, and residuum can often inhibit the sensitive chemistries by which sequencing-by-synthesis is performed. On the back end, data handling, analysis software packages, and pipelines can also impact sequencing outcomes. The current chapter will describe stepwise how acellular biofluid samples are prepared for small RNA sequencing. With regard to purification methods, we found that small RNA yield can be improved considerably by following the total RNA isolation protocol included with Ambion's mirVana PARIS Kit but modifying the organic extraction step. Specifically, after transferring the upper aqueous phase to a fresh tube, water is added to the residual material (interphase and lower organic layer) and again phase-separated. In contrast, all the protocols provided with the commercially available kits at the time of this chapter publication require only one organic extraction. This simple yet, as it turns out, quite useful modification allows access to previously inaccessible material. Potential benefits from these changes are a more comprehensive sample profiling of small RNA, as well as wider access to small volume samples, such as is typically available for acellular biofluids, which now can be prepared for small RNA sequencing on the Illumina platform.

  18. Mu süda on mägedes : [luuletused] / Robert Burns ; tlk. Ralf Parve, Ants Oras, Urmas Tõnisson, Kullo Vende, Mart Mäger, Georg Eduard Luiga, Friedrich Reinhold Kreutzwald, Karl August Hermann, Johannes Vau, Lydia Koidula, Ado Grenzstein

    Index Scriptorium Estoniae

    Burns, Robert, 1759-1796

    2006-01-01

    Sisu: Mu süda on mägedes ; Hing mägises Shotis ; Mu isa oli farmer ; Künnipoiss ; Noor neiu vanal mehel ; Vana Rob Morris ; Meg veskilt ; Veski-Meg ; Willie pruulis õlut ; John Odratera ; Hägisele ; Shotlased, nüüd seiske kindlad! ; Invernessi neid ; Kuradi tants aktsiisiametnikuga ; Macphersoni hüvastijätt / tlk. Ralf Parve ; Macphersoni hüvastijätt / / tlk. Ants Oras ; Nii ja naa ; Hiirele ; Eleegia Peg Nicholsoni surma puhul ; Vaga Willie palve ; Epitaaf vagale Willièle ; Värsid poeet Fergussoni portree alla ; Lõikuskuu laul ; On kaldal kõrkjad reas ; Mu arm on nagu ruske roos ; Mu arm... ; Oo, mu arm on verev, verev roos ; Esimene suudlus lahkudes ; Mõte karmist saatusest ; Oh näeksin tühja nõmme peal ; Kui näeksin ; "Kui üksi olles näeksid, neid..." ; Käies pikas rohus ; Värsiread ; Mind naine tihti taob ; Rõõmus lesk ; Willie Wastleì naine ; Mul on naine ; John Anderson / tlk. Urmas Tõnisson ; Jaan Anderson / tlk. Karl August Hermann ; Sügiselaul ; Kündja ja lill ; Kodu ; Ometi ; Kõige kiuste ; Kui õis, mis õitseb heinamaal ; Põlev armastus ; Hans ja Krõõt : [luuletused]. Epigramme: Raamatukoi ; Impromptu ; Puudus. Ammustele aegadele : [luuletus]. Autori kohta eluloolisi andmeid lk. 179

  19. "Ringlemise viis, millesse me kinni jäime : kurvad, heitlikud, lootusrikkad..." : [luuletused] / Joni Pyysalo ; soome keelest tõlkinud Marko Martinson

    Index Scriptorium Estoniae

    Pyysalo, Joni

    2007-01-01

    Sisu: "Ringlemise viis, millesse me kinni jäime: kurvad, heitlikud, lootusrikkad..." ; "Tundmatu linnu huige öisel nõmmel, kordub saamata vastust, vannitoa..." ; "Aga armastus on umbrohi: leiab üles iga võimaluse, iga prao, iga nõrga koha." ; "Oma nõrkuse asetad sõrmeotsa ta silmalaule..." ; "Sa magad kaua, äratad ikka veel selle igatsuse..." ; "Mitu korda ütleb ta lihtsat su nime ja ei jõua lõpuni..." ; "Su hingamine on meister, kui on kiirus peal..." ; "Haige männi latv nagu riidepuu konks..." ; "Aga mingid rahustid ei rahusta enam..." ; "Harva nii ärkvel, et saad aru, mis lahti..." ; "Kui 90-ndate muusikat mängitakse raadios nagu minevikku..." ; "Hommikud peavad olema õrnad siin, kus magatakse üksi..." ; "Kõik on võimalik..." ; "Laps uninub lõpuks..." ; "Maailm, et oleks ometi, millega kalibreerida..." ; "Naine püüab pimeduses nutta nii tasakesi..:" ; "Õunapuu all istub tool..." ; "Mälu tahab meile öelda..." ; "Nii nagu väikeste tissidega naisel ei lähe vaja rinnahoidjaid..." ; "Ma sätin su tekki paremini, nagu kohendaksin tuld..." ; "Mitte keegi pole mu tahtele niimoodi vastu hakanud..." ; "Kolgin vana maja keldris mutrivõtmega..." ; "Naine magab, nägu seina poole, mees tuleb teki alla, kõverdab põlved..." I-III ; "Katus sulab ööga kokku..."

  20. Terpenoid bioactive compound from Streptomyces rochei (M32): taxonomy, fermentation and biological activities.

    Science.gov (United States)

    Pazhanimurugan, Raasaiyah; Radhakrishnan, Manikkam; Shanmugasundaram, Thangavel; Gopikrishnan, Venugopal; Balagurunathan, Ramasamy

    2016-10-01

    The present study emphasized the production of biologically active terpenoid compound from Streptomyces rochei M32, which was isolated from Western Ghats ecosystem, South India. The presence of resistant genes like mecA, vanA of Staphylococcus aureus and bla SHV, bla TEM of Pseudomonas aeruginosa was confirmed by molecular studies. The isolated compound from Streptomyces rochei M32 inhibited wide range of standard and clinical drug resistant pathogens and enteric pathogens. The rice bran supplemented basal medium influenced the active compound production on 8th day of fermentation and yielded 1875 mg of crude extract from 10 g of rice bran substrate. Purification and characterization of crude ethyl acetate extract was achieved by preparative thin layer chromatography. The active fraction was identified as terpenoid class compound by chemical screening. Based on the results of spectral studies (NMR, LC-MS, FTIR, etc.), the active compound was tentatively identified as 1, 19-bis (3-hydroxyazetidin-1-yl) nonadeca-5, 14-diene-1, 8, 12, 19-tetraone with molecular weight 462.41 g/mol. Minimum inhibitory concentration value ranges between 7.6 and 31.2 µg/mL against test organisms was observed. The cytotoxicity results on cervical cancer (HeLa) cell line showed IC50 value of 2.034 µg/mL. The corresponding compound is not previously reported from any microbial resources.

  1. Evaluation of Verigene Blood Culture Test Systems for Rapid Identification of Positive Blood Cultures.

    Science.gov (United States)

    Kim, Jae-Seok; Kang, Go-Eun; Kim, Han-Sung; Kim, Hyun Soo; Song, Wonkeun; Lee, Kyu Man

    2016-01-01

    The performance of molecular tests using the Verigene Gram-Positive and Gram-Negative Blood Culture nucleic acid tests (BC-GP and BC-GN, resp.; Naosphere, Northbrook, IL, USA) was evaluated for the identification of microorganisms detected from blood cultures. Ninety-nine blood cultures containing Gram-positive bacteria and 150 containing Gram-negative bacteria were analyzed using the BC-GP and BC-GN assays, respectively. Blood cultures were performed using the Bactec blood culture system (BD Diagnostic Systems, Franklin Lakes, NJ, USA) and conventional identification and antibiotic-susceptibility tests were performed using a MicroScan system (Siemens, West Sacramento, CA, USA). When a single strain of bacteria was isolated from the blood culture, Verigene assays correctly identified 97.9% (94/96) of Gram-positive bacteria and 93.8% (137/146) of Gram-negative bacteria. Resistance genes mecA and vanA were correctly detected by the BC-GP assay, while the extended-spectrum β-lactamase CTX-M and the carbapenemase OXA resistance gene were detected from 30 cases cultures by the BC-GN assay. The BC-GP and BC-GN assays showed high agreement with conventional identification and susceptibility tests. These tests are useful for rapid identification of microorganisms and the detection of clinically important resistance genes from positive Bactec blood cultures.

  2. The frequency of resistance to antibiotics of most frequently isolated bacteria from blood cultures during the period 1997-2002

    Directory of Open Access Journals (Sweden)

    Mirović Veljko

    2004-01-01

    Full Text Available The aim of this study was to determine the frequency of resistance to antibiotics of the most frequently isolated bacteria from blood cultures of hospitalized patients during the period 1997-2002. The resistance to antibiotics was determined by disk diffusion method according to National Committee for Clinical Laboratory Standards procedures. The majority of staphylococci isolates were resistant to methicillin, and the proportion of methicillin-resistant Staphylococcus aureus was stable (76.8-81.6%, during the follow-up period. None of the staphylococci isolates were resistant to vancomycin, but there was a very high incidence of high-level resistance of enterococci to aminoglycosides (47.2-72.2%. In 1998, only one strain among enterococci was resistant to vancomycin (Enterococcus faecium, VanA fenotype. Enterococcus spp isolates expressed variable frequency of resistance to ampicillin (15-40.1% during the follow-up period. Among Enterobacteriaceae there were no isolates resistant to imipenem, but dramatic increase of the resistance to ceftriaxone was found from 35.9% in 1997 to 95.9% in 2002 (p<0.001. Extended spectrum beta-lactamases production was found in all the species of enterobacteria isolates. Resistance to imipenem was observed in Acinetobacter spp isolates in 2002 for the first time. Pseudomonas spp isolates expressed high and very variable resistance to all antibiotics tested during the follow-up period.

  3. Prevalence, outcome and risk factor associated with vancomycin-resistant Enterococcus faecalis and Enterococcus faecium at a Tertiary Care Hospital in Northern India.

    Science.gov (United States)

    Tripathi, A; Shukla, S K; Singh, A; Prasad, K N

    2016-01-01

    To determine the prevalence, genotype, risk factors and mortality in patients having vancomycin-resistant Enterococcus faecalis (VR E. faecalis) and Enterococcus faecium (VR E. faecium) infection or colonisation. A total of 1488 clinical isolates of E. faecalis and E. faecium were tested for vancomycin resistance by phenotypic (disk diffusion, E-test and broth micro-dilution test) and genotypic polymerase chain reaction methods. Records of all 1488 patients who had E. faecalis or E. faecium infection or colonisation were reviewed for the identification of host, hospital and medication related risk factors associated with VR E. faecalis and VR E. faecium. Of 1488 isolates, 118 (7.9%) were vancomycin-resistant and their distributions were as follows: E. faecalis=72 (61%) and E. faecium=46 (39%). All 118 vancomycin-resistant isolates were vanA genotype (minimum inhibitory concentration [MIC] to vancomycin ≥64 μg/ml and MIC to teicoplanin≥32 μg/ml) and none of the isolates was vanB genotype. Multivariate logistic regression analysis identified ventilator support and hospital stay for ≥48 h as independent risk factors associated with VR E. faecalis and VR E. faecium infection or colonisation. Hospital stay≥48 h was the only independent risk factor for mortality in patients infected with vancomycin-resistant enterococci. Strategies to limit the nosocomial infection especially in patients on ventilator support can reduce VRE incidence and related mortality.

  4. Prevalence of Virulence Factors and Vancomycin-resistant Genes among Enterococcus faecalis and E. faecium Isolated from Clinical Specimens.

    Science.gov (United States)

    Nasaj, Mona; Mousavi, Seyed Masoud; Hosseini, Seyed Mostafa; Arabestani, Mohammad Reza

    2016-06-01

    The aim of this study was to determine the occurrence of virulence determinants and vancomycin-resistant genes among Enterococcus faecalis and E. faecium obtained from various clinical sources. The study was performed on the 280 enterococcal isolated from clinical specimens in Hamadan hospitals, western Iran in 2012-14. Antibiotic susceptibility testing was performed using disk diffusion and Minimal Inhibitory Concentration (MIC) methods. The presence of vancomycin-resistant genes and virulence genes was investigated using PCR. Totally 280 enterococcal isolates were identified as follows: E. faecalis (62.5%), E. faecium (24%) and Enterococcus spp (13.5%). The results of antibiotic susceptibility testing showed that resistance rates to vancomycin and teicoplanin in E. faecalis and E. faecium isolates were 5% and 73%, respectively. Of Sixty vancomycin-resistant Enterococci strains, fifty-one isolates were identified as E. faecium (VREfm) and nine as E. faecalis (VREfs). Prevalence of esp, hyl, and asa1 genes were determined as 82%, 71.6%, and 100%, respectively in E. faecium strains; and 78%, 56/6%, and 97%, respectively in E. faecalis strains. The increased frequency of VREF, as seen with rapid rise in the number of vanA isolates should be considered in infection control practices.

  5. A polyclonal outbreak of bloodstream infections by Enterococcus faecium in patients with hematologic malignancies.

    Science.gov (United States)

    Alatorre-Fernández, Pamela; Mayoral-Terán, Claudia; Velázquez-Acosta, Consuelo; Franco-Rodríguez, Cecilia; Flores-Moreno, Karen; Cevallos, Miguel Ángel; López-Vidal, Yolanda; Volkow-Fernández, Patricia

    2017-03-01

    Enterococcus faecium causes bloodstream infection (BSI) in patients with hematologic malignancies (HMs). We studied the clinical features and outcomes of patients with HM with vancomycin-sensitive E faecium (VSE) and vancomycin-resistant E faecium (VRE) BSI and determined the genetic relatedness of isolates and circumstances associated with the upsurge of E faecium BSI. Case-control study of patients with HM and E faecium-positive blood culture from January 2008-December 2012; cases were patients with VRE and controls were VSE isolates. The strains were tested for Van genes by polymerase chain reaction amplification and we performed pulsed-field gel electrophoresis to determine genetic relatedness. Fifty-eight episodes of E faecium BSI occurred: 35 sensitive and 23 resistant to vancomycin. Mortality was 46% and 57%, attributable 17% and 40%, respectively. Early stage HM was associated with VSE (P = .044), whereas an episode of BSI within the 3 months before the event (P = .039), prophylactic antibiotics (P = .013), and vancomycin therapy during the previous 3 months (P = .001) was associated with VRE. The VanA gene was identified in 97% of isolates studied. E faecium isolates were not clonal. E faecium BSI was associated with high mortality. This outbreak of VRE was not clonal; it was associated with antibiotic-use pressure and highly myelosuppressive chemotherapy. Copyright © 2017 Association for Professionals in Infection Control and Epidemiology, Inc. Published by Elsevier Inc. All rights reserved.

  6. Proosa 1977. Kirjanduse argipäev / Mall Jõgi

    Index Scriptorium Estoniae

    Jõgi, Mall, 1947-

    2008-01-01

    Sisaldab joonealuseid märkusi ja bibliograafiat. - Varem ilmunud Looming 1978, nr. 2. - Ülevaade 1977. aastal ilmunud eesti proosast. Käsitletakse järgmisi autoreid ja nende teoseid: Ustav Mikelsaar "Ohver", Lembit Kivisaar "Vana diivan", Albert Uustulnd "Avali väraval", Asta Põldmäe "Me", Lilli Promet "Tüdrukud taevast", Nikolai Baturin "Leiud kajast", Aadu Hint "Oma saar", Paul Kuusberg "Linnukesega", Aimée Beekman "Sugupuu", Mari Saat "Mida teha emaga?", Veera Saar "Isa niinepuu", Villem Gross "Vaikimise motiivid", Jüri Tuulik "Meretagune asi", Aira Kaal "Teedla Riia aknas põleb tuli", Tõnis Lehtmets "Tume laas", Väino Ilus "Kinsli peremehed", Einar Maasik "Hispaania tants", Aino Müür "Linnuvere lugu", Jaan Kross "Kolme katku vahel" 3. osa, Endel Nirk "Kaanekukk", Paul Kuusberg "Võõras või õige mees", Toomas Vint "Perekondlikud mängud", Ine Viiding "Kümme käsku kaaskodanikele", Mats Traat "Irdinimene. Türgi oad", Teet Kallas "Õhtuvalgus", Teet Kallas "Insener Paberiti juhtum", Einar Maasik "Üksinda detsembris", Enn Vetemaa "Hõbedaketrajad", Herta Laipaik "Kummitus muusikatoas. Doktor Vallaku mõistatus", Raimond Kaugver "Külalisteraamat"

  7. Biocidal activity of metalloacid-coated surfaces against multidrug-resistant microorganisms.

    Science.gov (United States)

    Tétault, Nathalie; Gbaguidi-Haore, Houssein; Bertrand, Xavier; Quentin, Roland; van der Mee-Marquet, Nathalie

    2012-11-14

    The antimicrobial effects of a coating of molybdenum trioxide (MoO3) has been recently described. The metalloacid material produces oxonium ions (H3O+), which creates an acidic pH that is an effective, non specific antimicrobial. We determined the in vitro antimicrobial activity of molybdenum trioxide metalloacid-coated surfaces. Metalloacid-coated and non-coated (control) surfaces were contaminated by exposing them for 15 minutes to microbial suspensions containing 105 cfu/mL. Eleven microorganisms responsible for nosocomial infections were tested: two Staphylococcus aureus strains (the hetero-vancomycin intermediate MRSA Mu50 strain and a ST80-PVL-producing MRSA strain); a vancomycin-resistant vanA Enterococcus faecium strain; three extended-spectrum beta-lactamase-producing Enterobacteriaceae strains; a MBL-producing Pseudomonas aeruginosa strain; a multidrug-resistant Acinetobacter baumannii strain; a toxin-producing Clostridium difficile strain; and two fungi (Candida albicans and Aspergillus fumigatus). The assay tested the ability of the coated surfaces to kill microorganisms. Against all non-sporulating microorganisms tested, metalloacid-coated surfaces exhibited significant antimicrobial activity relative to that of the control surfaces within two to six hours after contact with the microorganisms (p biocidal effect of hydroxonium oxides against multidrug-resistant microorganisms may help limit environmental contamination between consecutive cleaning procedures.

  8. Biocidal activity of metalloacid-coated surfaces against multidrug-resistant microorganisms

    Directory of Open Access Journals (Sweden)

    Tétault Nathalie

    2012-11-01

    Full Text Available Abstract Background The antimicrobial effects of a coating of molybdenum trioxide (MoO3 has been recently described. The metalloacid material produces oxonium ions (H3O+, which creates an acidic pH that is an effective, non specific antimicrobial. We determined the in vitro antimicrobial activity of molybdenum trioxide metalloacid-coated surfaces. Methods Metalloacid-coated and non-coated (control surfaces were contaminated by exposing them for 15 minutes to microbial suspensions containing 105 cfu/mL. Eleven microorganisms responsible for nosocomial infections were tested: two Staphylococcus aureus strains (the hetero-vancomycin intermediate MRSA Mu50 strain and a ST80-PVL-producing MRSA strain; a vancomycin-resistant vanA Enterococcus faecium strain; three extended-spectrum beta-lactamase-producing Enterobacteriaceae strains; a MBL-producing Pseudomonas aeruginosa strain; a multidrug-resistant Acinetobacter baumannii strain; a toxin-producing Clostridium difficile strain; and two fungi (Candida albicans and Aspergillus fumigatus. The assay tested the ability of the coated surfaces to kill microorganisms. Results Against all non-sporulating microorganisms tested, metalloacid-coated surfaces exhibited significant antimicrobial activity relative to that of the control surfaces within two to six hours after contact with the microorganisms (p  Conclusions We suggest that, facing the continuing shedding of microorganisms in the vicinity of colonized or infected patients, the continuous biocidal effect of hydroxonium oxides against multidrug-resistant microorganisms may help limit environmental contamination between consecutive cleaning procedures.

  9. [Evaluation of the usefulness of selective chromogenic agar medium (chromID VRE) and multiplex PCR method for the detection of vancomycin-resistant enterococci].

    Science.gov (United States)

    Kim, Do-Hoon; Lee, Jae-Hee; Ha, Jung-Sook; Ryoo, Nam-Hee; Jeon, Dong-Seok; Kim, Jae-Ryong

    2010-12-01

    Accurate and early detection of vancomycin-resistant enterococci (VRE) is critical for controlling nosocomial infection. In this study, we evaluated the usefulness of a selective chromogenic agar medium and of multiplex PCR for detection of VRE, and both these techniques were compared with the conventional culture method for VRE detection. We performed the following 3 methods for detecting VRE infection in stool specimens: the routine culture method, culturing in selective chromogenic agar medium (chromID VRE, bioMérieux, France), and multiplex PCR using the Seeplex® VRE ACE Detection kit (Seegene Inc., Korea) with additional PCR for vanC genes. We isolated 109 VRE strains from 100 stool specimens by the routine culture method. In chromID VRE, all the isolates showed purple colonies, including Enterococcus gallinarum and E. raffinosus, which were later identified using the Vitek card. All VRE isolates were identified by the multiplex PCR method; 100 were vanA-positive E. faecium, 8 were vanA- and vanC-1-positive E. gallinarum, and 1 was vanA-positive E. raffinosus. For VRE surveillance, culturing the isolates in chromID VRE after broth enrichment appears to be an accurate, rapid, and easy method for routine screening test. Multiplex PCR is relatively expensive and needs skilled techniques for detecting VRE, but it can be an auxiliary tool for rapid detection of genotype during a VRE outbreak.

  10. Draama 2007. Töörahu / Ene Paaver

    Index Scriptorium Estoniae

    Paaver, Ene, 1963-

    2008-01-01

    Ülevaade 2007. aasta eesti draamast. Pikemalt käsitletakse järgmisi autoreid ja nende näidendeid: Mart Kivastik "Tõnu võitlused", Mart Kivastik "Eesti asi", Mart Kivastik "Sõdur", Mart Kivastik "Kuninga viimane lahing ja ülikooli algus", Mart Kivastik, "Nokia 3310", Andrus Kivirähk "Voldemar", Andrus Kivirähk "Uljas neitsi", Hendrik Toompere "Kommunisti surm", Andres Ehin "Saatuslik kihlvedu ehk Volquin Varbola all", Jan Rahman "Jõujaam", Jaak Kõdar "Muinassaar", Karl Berg "Teekond valguse riiki", Angela Randmets "Lembelood vanas linnas", Toomas Kall "Tähtede sadu", Lauri Vahtre "Muna EÜE", Urmas Espenberg "Kestvad kiiduavaldused", Kalju Saaber "Koduvõõrad", Indrek Hirv "Pauluse kiriku kellad", Katrin Saukas "Võlg", Heidi Sarapuu "Austatud Riigikogu liikmed!", Heidi Sarapuu "Helisev viis", Heidi Sarapuu "Ankur hiivata", Jakob Karu "Vanaema juures", Jim Ashilevi "Nagu poisid vihma käes", Jim Ashilevi "Portselansuits", Eva Koff "Mirr", Jaak Prints "Karaoke", Kati Murutar "Mina, naine!", Ott Aardam "Börs ja Börsitar", Andrus Kivirähk, Jan Rahman, Contra "Kõrts", Mart Kase "Perekond", Villu Tamme "Haned võlgu"; Tiit Ojasoo ja Ene-Liis Semperi "GEP ehk Gorjatshije estonskije parni"

  11. Enterococos resistentes a vancomicina: prevalencia y factores asociados a la colonización intestinal en pacientes oncológicos del Hospital de Niños de Córdoba Vancomycin-resistant enterococci: prevalence and factors associated with intestinal colonization in oncology patients from Hospital de Niños de Córdoba

    Directory of Open Access Journals (Sweden)

    A. L. Reale

    2009-06-01

    Full Text Available Los enterococos resistentes a vancomicina (EVR tienen un importante impacto sobre la población pediátrica oncológica. Los objetivos del presente estudio fueron conocer la prevalencia de colonización intestinal por EVR en pacientes oncológicos, identificar los factores de riesgo que predisponen a la colonización durante la hospitalización y determinar el perfil de resistencia de los EVR a diferentes agentes antimicrobianos. Se estudiaron todos los niños de entre un mes y 16 años de edad con enfermedad oncológica que ingresaron en el protocolo, de los internados desde octubre de 2006 hasta abril de 2007 en la Unidad de Oncohematología del Hospital de Niños la Santísima Trin idad de Córdoba (Argentina. Se investigó la colonización intestinal con EVR al ingreso, a las 72 h y semanalmente durante la internación. Se obtuvieron 333 muestras de 67 pacientes y se aislaron EVR de 12 pacientes, lo que arroja una prevalencia de 17,9%. De los 28 aislamientos estudiados (uno por paciente, 10 fueron Enterococcus faecium y 2 Enterococcus faecalis, ambos con fenotipo de resistencia VanA (CIM90 512 μg/ml a vancomicina y CIM90 256 μg/ml a teicoplanina. El uso de vancomicina (p=0,02, la duración de la neutropenia mayor de 7 días (p=0,03 y la internación prolongada (media 42,8 días (p=0,0001 fueron factores de riesgo asociados significativamente a la colonización con EVR. En función de estos resultados consideramos necesaria la vigilancia epidemiológica y la implementación de medidas de prevención y control.Vancomycin-resistant enterococci (VRE have an important impact on pediatric oncology population. The objectives of this study were: to know the prevalence of VRE intestinal colonization in oncology patients, to identify the risk factors that predispose hospitalized patients to VRE intestinal colonization , and to determine the VRE resistance profile to different antimicrobial agents. We studied all children with oncological disease

  12. Possible Connection between a Widely Disseminated Conjugative Gentamicin Resistance (pMG1-Like) Plasmid and the Emergence of Vancomycin Resistance in Enterococcus faecium

    Science.gov (United States)

    Tomita, Haruyoshi; Pierson, Carl; Lim, Suk Kyung; Clewell, Don B.; Ike, Yasuyoshi

    2002-01-01

    A total of 640 vancomycin-resistant Enterococcus faecium (VRE) isolates, which were obtained between 1994 and 1999 from the Medical School Hospital of the University of Michigan, Ann Arbor, were used in this study. Of the 640 strains, 611 and 29 were VanA and VanB VRE, respectively, based on PCR analysis. Four hundred ninety-two (77%) of the strains exhibited resistance to concentrations of gentamicin from 64 μg/ml (MIC) to more than 1,024 μg/ml (MIC). The gentamicin resistance of each of 261 (53%) of the 492 gentamicin-resistant strains was transferred to E. faecium at a frequency of about 10−5 to 10−6 per donor cell in broth mating. More than 90% of vancomycin resistances of the 261 strains cotransferred with the gentamicin resistances to E. faecium strains by filter mating. The conjugative gentamicin resistance plasmids were identified and were classified into five types (A through E) with respect to their EcoRI restriction profiles. The transfer frequencies of each type of plasmid between E. faecium strains or Enterococcus faecalis strains were around 10−3 to 10−5 per donor cell or around 10−6 to 10−7 per donor cell, respectively, in broth mating. Type A and type B were the most frequently isolated, at an isolation frequency of about 40% per VRE isolate harboring the gentamicin resistance conjugative plasmid. The plasmids did not show any homology in Southern hybridization with the pheromone-responsive plasmids and broad-host-range plasmids pAMβ1 and pIP501. The EcoRI or NdeI restriction fragments of each type of plasmids hybridized to the conjugative gentamicin resistance plasmid pMG1 (65.1 kb), which was originally isolated from an E. faecium clinical isolate, and transfer efficiently in broth mating. PMID:12202574

  13. Emergence of linezolid- and vancomycin-resistant Enterococcus faecium in a department for hematologic stem cell transplantation

    Directory of Open Access Journals (Sweden)

    M. Krull

    2016-09-01

    Full Text Available Abstract Background Prevalence of vancomycin-resistant enterococci has increased in Germany. Here, we report the cluster of linezolid- and vancomycin-resistant Enterococcus faecium (LVRE in a German department for hematologic stem cell transplantation (HSCT. Methods In this retrospective analysis we included all patients with LVRE in a university-based department for HSCT in 2014 and 2015. Patients chart reviews were used to investigate the epidemiology and clinical outcome. Available LVRE isolates underwent detailed microbiological characterization and genotyping by pulsed-field gel electrophoresis (PFGE. Results In total, 20 patients with LVRE were identified within the observed time period. All except two patients underwent allogeneic HSCT. Surveillance culture results from incoming patients and chart review revealed that 10 of 20 patients were colonized at hospital admission. Eight of 10 patients with in-hospital acquired LVRE had previous linezolid treatment. Analysis of spatio-temporal patterns showed no evidence for LVRE patient-to-patient or environment-to-patient transmission within the HSCT department. In five cases (25 % LVRE bloodstream infection occurred. Nine LVRE isolates could be saved for characterization. Eight isolates carried vanA, one isolate vanB. PFGE analysis showed that four different LVRE clones were responsible for the cluster. One single genotype was present in six LVRE isolates whereupon the corresponding patients were all referred from the same hospital to the HSCT department. Conclusions This is the first report demonstrating the emergence of LVRE in a German HSCT department. (LVRE screening at patients’ admission and appropriate infection control strategies were sufficient to prevent any transmission. Further studies in this predisposed patient collective are warranted.

  14. Lactobacillus pentosus B231 Isolated from a Portuguese PDO Cheese: Production and Partial Characterization of Its Bacteriocin.

    Science.gov (United States)

    Guerreiro, Joana; Monteiro, Vitor; Ramos, Carla; Franco, Bernadette Dora Gombossy de Melo; Martinez, Rafael Chacon Ruiz; Todorov, Svetoslav Dimitrov; Fernandes, Paulo

    2014-06-01

    Bacteriocin B231 produced by Lactobacillus pentosus, isolated from an artisanal raw cow's milk protected designation of origin Portuguese cheese, is a small protein with an apparent relative mass of about 5 kDa and active against a large number of Listeria monocytogenes wild-type strains, Listeria ivanovii and Listeria innocua. Bacteriocin B231 production is highly dependent on the type of the culture media used for growth of Lact. pentosus B231. Replacement of glucose with maltose yielded the highest bacteriocin production from eight different carbon sources. Similar results were recorded in the presence of combination of glucose and maltose or galactose. Production of bacteriocin B231 reached maximal levels of 800 AU/ml during the stationary phase of growth of Lact. pentosus B231 in MRS broth at 30 °C. Bacteriocin B231 (in cell-free supernatant) was sensitive to treatment with trypsin and proteinase K, but not affected by the thermal treatment in range of 55-121 °C, or freezing (-20 °C). Bacteriocin production and inhibitory spectrum were evaluated. Gene encoding plantaricin S has been detected in the genomic DNA. Virulence potential and safety of Lact. pentosus B231 were assessed by PCR targeted the genes gelE, hyl, asa1, esp, cylA, efaA, ace, vanA, vanB, hdc1, hdc2, tdc and odc. The Lact. pentosus B231 strains harbored plantaricin S gene, while the occurrence of virulence, antibiotic resistance and biogenic amine genes was limited to cytolysin, hyaluronidase, aggregation substance, adhesion of collagen protein, gelatinase, tyrosine decarboxylase and vancomycin B genes.

  15. Resistance mechanism and clinical characteristics of linezolid-resistant Enterococcus faecium: a single center study in Korea.

    Science.gov (United States)

    Cho, Sun Young; Kim, Hye Mee; Chung, Doo Ryeon; Kim, So Hyun; Huh, Hee Jae; Kang, Cheol-In; Peck, Kyong Ran; Lee, Nam Yong; Song, Jae-Hoon

    2017-09-20

    This study aimed to determine the prevalence of linezolid-resistant vancomycin-resistant Enterococcus (LR-VRE) and investigate the mechanisms for linezolid resistance with clinical and microbiological characterization. All vancomycin-resistant Enterococcus faecium (VREF) isolates from blood and rectal swab cultures during 2012-2015 were tested for linezolid resistance. LR-VREF isolates were tested for antimicrobial susceptibility, genes for glycopeptide resistance, and virulence genes. Multilocus sequence typing (MLST) and pulsed field gel electrophoresis (PFGE) were performed. The isolates were tested for the known mechanisms of linezolid resistance. Among 389 VREF isolates, seven (1.8%) were found to be resistant to linezolid. All LR-VREF isolates carried the vanA gene. Five isolates had both hyl and esp. The isolates were susceptible to tigecycline, daptomycin and quinupristin-dalfopristin except one with daptomycin resistance. Two LR-VREF isolates recovered from patients with previous linezolid exposure contained the G2576T mutation in 23S rRNA and exhibited high-level resistance to linezolid (MIC >64mg/L). The other five isolates recovered from linezolid-naive patients revealed no known linezolid resistance mechanism and exhibited low-level resistance to linezolid (MIC 8 to 16mg/L). Plasmid-mediated genes encoding cfr or optrA were not detected. LR-VREF isolates were represented by six different sequence types, which belonged to hospital lineages, and were assigned to seven PFGE types. The prevalence of LR-VREF in our center was low. Both linezolid exposure and horizontal transmission appear to be responsible for acquisition of LR-VREF in hospitalized patients. Prudent use of linezolid and improved infection control strategies are needed to limit the spread of LR-VREF. Copyright © 2017. Published by Elsevier Ltd.

  16. Detection of vancomycin-resistant enterococci from faecal samples of Iberian wolf and Iberian lynx, including Enterococcus faecium strains of CC17 and the new singleton ST573.

    Science.gov (United States)

    Gonçalves, Alexandre; Igrejas, Gilberto; Radhouani, Hajer; López, María; Guerra, Ana; Petrucci-Fonseca, Francisco; Alcaide, Eva; Zorrilla, Irene; Serra, Rodrigo; Torres, Carmen; Poeta, Patrícia

    2011-12-01

    The aim of this study was to perform the molecular characterization of vancomycin resistant enterococci (VRE) within the faecal flora of Iberian wolf and Iberian lynx. The association with other resistance genes and the detection of virulence genes were also analysed. From 2008 to 2010, 365 faecal samples from Iberian wolf and Iberian lynx were collected and tested for VRE recovery. Mechanisms of resistance to vancomycin and other antibiotics, as well as genes encoding virulence factors were detected through PCR. Multilocus Sequence Typing (MLST) was performed for Enterococcus faecium strains. VRE were recovered in 8 of the 365 analysed samples. The vanA gene was identified in two E. faecium isolates recovered from Iberian wolf faecal samples and the remaining six showed intrinsic resistance (3 vanC1-E. gallinarum and 3 vanC2-E. casseliflavus, from Iberian wolf and Iberian lynx faecal samples, respectively). One vanA-containing isolate showed tetracycline and erythromycin resistance [with erm(B) and tet(L) genes] and the other one also exhibited ampicillin and kanamycin resistance [with erm(B), tet(M) and aph(3')-III genes]. One of the vanA-isolates revealed a new sequence type named ST573 and the other one belonged to the CC17 clonal complex (ST18). The hyl gene was detected in one E. casseliflavus and three E. gallinarum but not among vanA-positive isolates, and the occurrence of cylA and cylL genes was confirmed in two E. casseliflavus isolates. A low prevalence of VRE has been detected in faecal samples of Iberian wolf and Iberian lynx and strains with an acquired mechanism of resistance to vancomycin have not been detected among Iberian lynx. Copyright © 2011 Elsevier B.V. All rights reserved.

  17. 212ʻ Fahrenheiti : eesti luule 2006 / Piret Bristol

    Index Scriptorium Estoniae

    Bristol, Piret, 1968-

    2008-01-01

    Järgmistest 2006. a. ilmunud luulekogudest: Ryytle, Indrek. Inglid rokijaamas. Puhja : I. Rüütle ; Runnel, Hando. Viru veri ei värise. Tartu : Ilmamaa ; Vabat, Martin. Mina olengi kirjandusklassik. Tartu : Eesti Kirjanduse Selts ; Korts, Eliina. Lööklaused murravad metsi. Tartu : Eesti Kirjanduse Selts ; Koff, Indrek. Vana laul. Luige : Verb ; Teede, Andra. Takso Tallinna taevas. Luige : Verb ; Vusser, Kaspar. Vusserduste võnked. Tallinn : P. Kukk ; Elbing, Andrus. Siin Beebilõust, tere! : häired pimelinna tänavalt = Ciao, it's Babyface : trouble from the streets of a blind city. Tallinn : Epifanio ; Hint, Miina. Vabaduse vang, ehk, Põrandaalune kirjandus. Tallinn : MR ; Jüriado, Tiiu. Luule on ime. Habaja : Kentaur ; Ligi, Katre. Naabrivalve. Tartu : Ilmamaa ; Krull, Hasso. Talv. Tallinn : Tuum ; Mets, Mae. Pühapäeval on reede. Tartu : Ilmamaa ; Ploom, Ülar. Porr ja sorry. Tallinn : Tuum ; Hirv, Indrek, Surmapõletaja. Tallinn : Tuum ; Piir, Milvi Martina. Kõrkjavaas. Tartu : Fantaasia ; Soomets, Triin. Väljas. Tallinn : Tuum ; Mathura. Kohalolu. Rapla : Allikäärne ; Afanasjev, Vahur. Katedraal Emajões. Tartu ; Brüssel : ID Salong//Sild, Ivar. Spermaga ja puha. Pärnu : Jumalikud Ilmutused ; Meiel, Kaupo. Polügrafisti käsiraamat. Pärnu : Jumalikud Ilmutused ; Kivisildnik, Sven. Vägistatud jäämägi. Pärnu : Jumalikud Ilmutused ; Arder, Ott. Luule sünnib kus sünnib kui sünnib / koost. Leelo Tungal. Tallinn : Tänapäev ; Kompus, Marko. Vallaliste jõgede tõkkejooksja. Tartu : M. Kompus

  18. Žanr : suvelavastus? / Jaak Allik

    Index Scriptorium Estoniae

    Allik, Jaak, 1946-

    2007-01-01

    Kahekümnest suvisest teatriõhtust: Carlo Gozzi "Ronk" Tallinna Linnateatri lavaaugus (lavastaja Elmo Nüganen), Komöödiateatri suvetuuri lavastus "Elu parim puhkus" (lavastaja Ago-Endrik Kerge), muusikal "Tutvumiskuulutus" (lavastaja Mait Malmsten), Bernard Kangermanni laulumäng "Naabri Mari" (lavastaja Margus Vaher) ja Mara Zalite "Sirelikassid" (lavastaja Peeter Tammearu) Ugalas, Paul Barzi komöödia "Võimalik kohtumine" (lavastaja Raivo Trass) Vanemuise teatri suvelavastus Muhumaal Pädaste mõisas, muusikaline estraadietendus "Tähtede sadu", Endla "Tsirkus!!!" Paikuse-Reiu vabaõhulaval (autor ja lavastaja Tiit Palu), Andrus Kivirähki näidend "Uljas Neitsi" Laulasmaa Resort' rannametsas (lavastaja Taavi Teplenkov), Marti Kivastik "Eesti asi" (lavastaja Ingomar Vihmar) MTÜ Ühendus R.A.A.A.M. Viinistu kunstimuuseumi vabaõhulaval, Emajõe Suveteatri suvelavastus "Jumalaema kiriku kellamees" Victor Hugo romaani järgi (lavastaja Andres Dvinjaninov), "Muna EÜE ehk Viimane kava" (autor Lauri Vahtre, lavastaja Andrus Vaarik) Suure Munamäe vabaõhulaval, Aino Kalda "Reigi õpetaja" Kuressaares (lavastaja ja dramatiseerija Aare Toikka), Vana Baskini Teateris Pierre Chesnot "Kes aevastas?" (lavastaja Eino Baskin), Eduard Vilde romaani "Prohvet Maltsvet" ja vene kirjaniku Viktor Pelevini romaani aineil sündinud lavastus "Proffet" Albu vallas Kukenoosi rehealuses (lavastaja Mart Koldits), Enn Vetemaa "Roosiaed" Raasiku vallas Kiviloo mõisas (lavastaja Eva Klemets), Jim Ashilevi "Portselansuits" (lavastaja Lauri Lagle) Eesti Teatriliidu saalis, Andres Keili "Rooside sõda" Leigo Järveteatris (lavastaja Tõnu Lensment), Võru Teatriateljee suvelavastus Madis Kõivu "Castrozza ehk illusionist" (lavastaja Taago Tubin), Villu Tamme "Haned võlgu" (lavastaja ja kunstnik Jaanika Juhanson) Teatrilabor Von Krahli teatris

  19. "Daños, engaños y desengaños, frutos son de los años": the semantic value of rhyme in the Spanish baroque sonnets on the transience of life

    Directory of Open Access Journals (Sweden)

    Špela Oman

    2012-12-01

    Full Text Available One of the most recurring Baroque themes is without a doubt the topic of tempus fugit, which inspired numerous contemplations about the ephemeral nature of life, the brevity of the earthly existence and the omnipresence of death. The obsession with the passing of time in the Spanish Baroque sonnets on the ephemerality of life can also be observed on the level of rhyme, which far from being merely an ornamental item, actually contributes important semantic value to the poem. Rhymes such as años/daños/engaños/desengaños or mañana/vana, jornada/nada reappear almost obsessively throughout the corpus of Baroque poetry and form semantic knots that highlight the principal theme of the sonnet. Similarly, pairs of words such as encina/ruina, vida/desvanecida, humano/gusano, besos/huesos, are closely related despite different meanings, drawing attention to the devastating eff ects of the passage of time. The insatiable quest for suggestive rhymes by Golden Age poets is doubtless due to the aesthetic orientation of Baroque art that tends to combine decorative virtuosity with the intellectual subtlety. The semantic rhyme thus functions as one of the axis of the meaning, as an echo resonating the poetic message, and likewise as a means of fulfilling the Baroque search for thematic unity. The rhyme in the Baroque sonnets is integrated thematically with the text, in the same manner as a pillar supporting a Baroque facade is subjected to the effect of absolute architectural unity.

  20. Pyrosequencing-based comparative genome analysis of the nosocomial pathogen Enterococcus faecium and identification of a large transferable pathogenicity island

    Directory of Open Access Journals (Sweden)

    Bonten Marc JM

    2010-04-01

    Full Text Available Abstract Background The Gram-positive bacterium Enterococcus faecium is an important cause of nosocomial infections in immunocompromized patients. Results We present a pyrosequencing-based comparative genome analysis of seven E. faecium strains that were isolated from various sources. In the genomes of clinical isolates several antibiotic resistance genes were identified, including the vanA transposon that confers resistance to vancomycin in two strains. A functional comparison between E. faecium and the related opportunistic pathogen E. faecalis based on differences in the presence of protein families, revealed divergence in plant carbohydrate metabolic pathways and oxidative stress defense mechanisms. The E. faecium pan-genome was estimated to be essentially unlimited in size, indicating that E. faecium can efficiently acquire and incorporate exogenous DNA in its gene pool. One of the most prominent sources of genomic diversity consists of bacteriophages that have integrated in the genome. The CRISPR-Cas system, which contributes to immunity against bacteriophage infection in prokaryotes, is not present in the sequenced strains. Three sequenced isolates carry the esp gene, which is involved in urinary tract infections and biofilm formation. The esp gene is located on a large pathogenicity island (PAI, which is between 64 and 104 kb in size. Conjugation experiments showed that the entire esp PAI can be transferred horizontally and inserts in a site-specific manner. Conclusions Genes involved in environmental persistence, colonization and virulence can easily be aquired by E. faecium. This will make the development of successful treatment strategies targeted against this organism a challenge for years to come.

  1. Occurrence of transferable antibiotic resistances in commercialized ready-to-eat mealworms (Tenebrio molitor L.).

    Science.gov (United States)

    Osimani, Andrea; Cardinali, Federica; Aquilanti, Lucia; Garofalo, Cristiana; Roncolini, Andrea; Milanović, Vesna; Pasquini, Marina; Tavoletti, Stefano; Clementi, Francesca

    2017-12-18

    The present study aimed to assess the occurrence of transferable determinants conferring resistance to tetracyclines, macrolide-lincosamide-streptogramin B, vancomycin, beta-lactams, and aminoglycosides in 40 samples of commercialized edible mealworms (Tenebrio molitor L.) purchased from European Union (EU) and non-EU producers. A high prevalence of tet(K) was observed in all of the samples assayed, with percentages of PCR-based positivity that ranged from 80% (samples from Thailand) to 100% (samples from the Netherlands, Belgium and France). For macrolides, erm(B) prevailed, being detected in 57.5% of the samples assayed, whereas erm(A) and erm(C) were detected with lower frequencies. Genes for resistance to vancomycin were only detected in samples produced in France and Belgium, with 90% and 10% of the samples being positive for vanA, respectively. Beta-lactamase genes were found with low occurrence, whereas the gene aac-aph, conferring high resistance to aminoglycosides, was found in 40% of the samples produced in the Netherlands and Belgium and 20% of the samples produced in Thailand. The results of Principal Coordinate Analysis and Principal Component Analysis depicted a clean separation of the samples collected from the four producers based on the distribution of the 12 AR determinants considered. Given the growing interest on the use of mealworms as a novel protein source, AR detection frequencies found in the present study suggest further investigation into the use of antibiotics during rearing of this insect species and more extensive studies focused on the factors that can affect the diffusion of transferable ARs in the production chain. Until such studies are completed, prudent use of antibiotics during rearing of edible insects is recommended. Copyright © 2017 Elsevier B.V. All rights reserved.

  2. Performance Evaluation of the Verigene Gram-Positive and Gram-Negative Blood Culture Test for Direct Identification of Bacteria and Their Resistance Determinants from Positive Blood Cultures in Hong Kong.

    Science.gov (United States)

    Siu, Gilman K H; Chen, Jonathan H K; Ng, T K; Lee, Rodney A; Fung, Kitty S C; To, Sabrina W C; Wong, Barry K C; Cheung, Sherman; Wong, Ivan W F; Tam, Marble M P; Lee, Swing S W; Yam, W C

    2015-01-01

    A multicenter study was conducted to evaluate the diagnostic performance and the time to identifcation of the Verigene Blood Culture Test, the BC-GP and BC-GN assays, to identify both Gram-positive and Gram-negative bacteria and their drug resistance determinants directly from positive blood cultures collected in Hong Kong. A total of 364 blood cultures were prospectively collected from four public hospitals, in which 114 and 250 cultures yielded Gram-positive and Gram-negative bacteria, and were tested with the BC-GP and BC-GN assay respectively. The overall identification agreement for Gram-positive and Gram-negative bacteria were 89.6% and 90.5% in monomicrobial cultures and 62.5% and 53.6% in polymicrobial cultures, respectively. The sensitivities for most genus/species achieved at least 80% except Enterococcus spp. (60%), K.oxytoca (0%), K.pneumoniae (69.2%), whereas the specificities for all targets ranged from 98.9% to 100%. Of note, 50% (7/14) cultures containing K.pneumoniae that were missed by the BC-GN assay were subsequently identified as K.variicola. Approximately 5.5% (20/364) cultures contained non-target organisms, of which Aeromonas spp. accounted for 25% and are of particular concern. For drug resistance determination, the Verigene test showed 100% sensitivity for identification of MRSA, VRE and carbapenem resistant Acinetobacter, and 84.4% for ESBL-producing Enterobacteriaceae based on the positive detection of mecA, vanA, blaOXA and blaCTXM respectively. Overall, the Verigene test provided acceptable accuracy for identification of bacteria and resistance markers with a range of turnaround time 40.5 to 99.2 h faster than conventional methods in our region.

  3. Molecular analysis and distribution of multidrug-resistant Enterococcus faecium isolates belonging to clonal complex 17 in a tertiary care center in Mexico City

    Science.gov (United States)

    2013-01-01

    Background Enterococcus faecium has recently emerged as a multidrug-resistant nosocomial pathogen involved in outbreaks worldwide. A high rate of resistance to different antibiotics has been associated with virulent clonal complex 17 isolates carrying the esp and hyl genes and the purK1 allele. Results Twelve clinical vancomycin-resistant Enterococcus faecium (VREF) isolates were obtained from pediatric patients at the Hospital Infantil de México Federico Gómez (HIMFG). Among these VREF isolates, 58.3% (7/12) were recovered from urine, while 41.7% (5/12) were recovered from the bloodstream. The VREF isolates showed a 100% rate of resistance to ampicillin, amoxicillin-clavulanate, ciprofloxacin, clindamycin, chloramphenicol, streptomycin, gentamicin, rifampicin, erythromycin and teicoplanin. In addition, 16.7% (2/12) of the isolates were resistant to linezolid, and 66.7% (8/12) were resistant to tetracycline and doxycycline. PCR analysis revealed the presence of the vanA gene in all 12 VREF isolates, esp in 83.3% (10/12) of the isolates and hyl in 50% (6/12) of the isolates. Phylogenetic analysis via molecular typing was performed using pulsed-field gel electrophoresis (PFGE) and demonstrated 44% similarity among the VREF isolates. MLST analysis identified four different sequence types (ST412, ST757, ST203 and ST612). Conclusion This study provides the first report of multidrug-resistant VREF isolates belonging to clonal complex 17 from a tertiary care center in Mexico City. Multidrug resistance and genetic determinants of virulence confer advantages among VREF in the colonization of their host. Therefore, the prevention and control of the spread of nosocomial infections caused by VREF is crucial for identifying new emergent subclones that could be challenging to treat in subsequent years. PMID:24330424

  4. Isolation of secreted microRNAs (miRNAs) from cell-conditioned media.

    Science.gov (United States)

    Glynn, Claire L; Khan, Sonja; Kerin, Michael J; Dwyer, Roisin M

    2013-01-01

    MicroRNAs (miRNAs) have been found to be stable in the circulation of cancer patients raising their potential as circulating biomarkers of disease. The specific source and role, however, of miRNAs in the circulation is unknown and requires elucidation to determine their true potential. In this study, along with primary tissue explants and primary stromal cells, three breast cancer cell lines were employed, including T47D, MDA-MB-231 and SK-BR-3. Tissue explants were harvested in theatre, with informed patient consent, and included tumour, tumour associated normal, and diseased lymph node samples. Cell-conditioned media containing all factors secreted by the cells were harvested. MiRNAs were extracted from samples using 5 different extraction techniques including the blood protocol, RNeasy® (Qiagen), miRNeasy®mini kit (Qiagen), mirVana™ isolation kit (Ambion) and RNAqueous® kit (Ambion). MiRNAs were successfully isolated from all media samples collected from cell lines, primary cells and fresh tissue explants. However, there was remarkable variation in yield depending on the extraction method used. Aliquots of the same samples were extracted, revealing the two column extraction protocol of the mirVana® miRNA isolation kit to be the most suitable approach. A range of miRNAs, including miR-16, miR-195, miR-497 and miR-10b, were successfully amplified. While miR-16 and miR-195 were detected in media from both cell lines and tissue explants, miR-497 and miR-10b were only detected in secretions from whole tissue explants. The ability to achieve reliable and reproducible miRNA yields from cell-conditioned media is vital for the successful amplification of miRNAs by RQ-PCR.

  5. Comparison of RNA extraction kits and histological stains for laser capture microdissected prostate tissue.

    Science.gov (United States)

    Kolijn, Kimberley; van Leenders, Geert J L H

    2016-01-07

    Laser capture microdissection offers unique possibilities for the isolation of specific cell populations or histological structures. However, isolation of RNA from microdissected tissue is challenging due to degradation and minimal yield of RNA during laser capture microdissection (LCM). Our aim was to optimize the isolation of high-quality RNA from laser capture microdissected fresh frozen prostate tissue on the level of staining and RNA extraction. Cresyl violet and haematoxylin were compared as histological stains for LCM. While RNA quality was similar for cresyl violet (median RIN 7.4) and haematoxylin (median RIN 7.6), tissue morphology was more detailed with cresyl violet as compared to haematoxylin. RNA quality from the following kits was compared: RNeasy(®) Micro (median RIN 7.2), miRNeasy Mini (median RIN 6.6), Picopure(®) (median RIN 6.0), mirVana™ miRNA (median RIN 6.5) and RNAqueous(®)-Micro (median RIN 6.3). RNA quality from microdissected samples with either the RNeasy Micro or miRNeasy Mini kit, was comparable to RNA isolated directly from whole tissue slices (median RIN 7.5, p = 0.09). Isolated RNA from benign and prostate cancer microdissected tissue demonstrated that RNA quality can vary between regions from the same clinical sample. Additionally, RNA quality (r = 0.89), but not quantity (r = 0.69) could be precisely measured with the Agilent Bioanalyzer. We demonstrate that staining with cresyl violet results in the isolation of high quality RNA from laser capture microdissected tissue with high discriminative morphology. The RNeasy Micro and miRNeasy Mini RNA extraction kits generated the highest quality RNA compared to Picopure, mirVana and RNAqueous with minimal loss of RNA quality during LCM.

  6. Ma tean, et päike liigub : [luuletused] / Hando Runnel

    Index Scriptorium Estoniae

    Runnel, Hando, 1938-

    1998-01-01

    Sisu: Ma tean et päike liigub ; Ma tean, et ma tulen kord jälle ; Jaanipäevaks kõrgeks kasvab rohi ; Luigelaul ; Pühapäev lastepargis ; Kui päike loojub ; Kohvik ; "Kui veel..." ; Muruks kummel, muruks linnurohi ; Ei mullast sul olegi enam suurt lugu ; Vanaema ; Hauakiri ; Testament ; Üks veski seisab vete pääl ; Kui sa tuled ; Töölispoiss ; Sõbrad ; Mõnitus ; Naine naturaalne ; Kõigevägevam ja kõigelahjem ; Kehv kõhn poiss ; Keldrikakand ; Loogikaloksed ; Tume rahvas ; Võllalaul ; Kodu ; Ei ole ma käinud Komorras ; Mäletaja ; Hingede aeg ; Kui pidud on peetud ; Muusikat olgu ; Kaua sa kannatad ; Üle maa, üle mäda ; Jutt ; "Mis on me pääl, kas jumalate viha..." ; Jälle hakkab ; Müüt ; Vähid ; Muuda ennast ; Mõistmine ; Lindprii ; Kolm külameest 1-3 ; Meie õnn on kindel ; Ei saa me läbi Lätita ; Saadik ; Jälle ; Unistus ; Mets on kõrge ; "Marssis majja majuline..." ; "Üks väga vana rahvas..." ; "Ma polegi isa näinud..." ; "Ilus on ikkagi isamaa pale ; "Kiri algab kirikust..." ; "Mehepoegi läheb pätiks..." ; "Isa oli joodik..." "Hiired veavad pabereid..." ; "Talisman on Talli-Mannil..." ; "Täna ma luuletan läbi öö..." ; "Küll ma ootasin sind tollel tuulisel õhtul..." ; "Me liigume loogeldes loojangu poole... 1-10"

  7. Rapid detection of Gram-positive organisms by use of the Verigene Gram-positive blood culture nucleic acid test and the BacT/Alert Pediatric FAN system in a multicenter pediatric evaluation.

    Science.gov (United States)

    Sullivan, K V; Turner, N N; Roundtree, S S; Young, S; Brock-Haag, C A; Lacey, D; Abuzaid, S; Blecker-Shelly, D L; Doern, C D

    2013-11-01

    Assays that expedite the reporting of organism identification and antibiotic susceptibility status in positive blood cultures can fast track interventions that improve clinical outcomes. We evaluated the Verigene Gram-positive blood culture nucleic acid test (BC-GP) in two pediatric hospitals. Positive BacT/Alert Pediatric FAN blood cultures with Gram-positive organisms were tested using the BC-GP in tandem with routine laboratory procedures. To test organisms underrepresented in the clinical blood culture evaluation, blood culture bottles were spiked with diluted organism suspensions at concentrations of 10 to 100 CFU per milliliter. A total of 249 Gram-positive bacterial isolates were recovered from 242 blood cultures. The BC-GP detected Staphylococcus aureus, methicillin-susceptible S. aureus, and methicillin-resistant S. aureus with sensitivities of 100%, 99%, and 100% and specificities of 100%, 100%, and 99.5%, respectively. The BC-GP detected Staphylococcus epidermidis, methicillin-susceptible S. epidermidis, and methicillin-resistant S. epidermidis with sensitivities of 95%, 80%, and 96%, respectively, and 100% specificity. The BC-GP correctly identified 14/15 cases of Enterococcus faecalis and Enterococcus faecium bacteremia and 9 cases of Streptococcus pneumoniae. It misidentified 5/15 clinical blood cultures with Streptococcus mitis/Streptococcus oralis and 1/3 blood cultures spiked with Streptococcus anginosus group as S. pneumoniae. The BC-GP detected a case of Streptococcus pyogenes bacteremia but failed to detect 2/3 clinical blood cultures with Streptococcus agalactiae. BC-GP's rapid accurate detection of Staphylococcus spp., E. faecium, and E. faecalis and its ability to ascertain mecA, vanA, and vanB status may expedite clinical decisions pertaining to optimal antibiotic use. False-positive S. pneumoniae results may warrant reporting of only "Streptococcus spp." when this organism is reported by the BC-GP.

  8. Longitudinal Association between Short Sleep, Body Weight, and Emotional and Learning Problems in Hispanic and Caucasian Children

    Science.gov (United States)

    Silva, Graciela E.; Goodwin, James L.; Parthasarathy, Sairam; Sherrill, Duane L.; Vana, Kimberly D.; Drescher, Amy A.; Quan, Stuart F.

    2011-01-01

    Study Objective: To determine the impact of lower amounts of childhood sleep assessed by polysomnogram on development of obesity, being anxious or depressed, or having learning problems 5 years later. Design: Prospective cohort. Participants: Subjects were 304 community participants from the Tucson Children's Assessment of Sleep Apnea study, aged 6–12 years old at baseline. Measurements and Results: Children were classified according to baseline sleep as those who slept ≥ 9 h/night, those who slept > 7.5 to obese (≥ 85th BMI percentile), obese (≥ 95th BMI percentile), anxious or depressed, and learning problems at follow-up were assessed according to baseline sleep categories. Children who slept ≤ 7.5 h/night had higher odds of being obese (OR = 3.3, P children who slept ≥ 9 h/night. Borderline significance for overweight/obese (OR = 2.2, P children who slept ≤ 7.5 h/night as compared to those who slept ≥ 9 h/night. A mean increase in BMI of 1.7 kg/m2 (P = 0.01) over the 5 years of follow-up was seen for children who slept ≤ 7.5 h/night compared to those who slept ≥ 9 h/night. These relationships did not differ between Hispanic and Caucasian children. Conclusions: Children with reduced amounts of sleep (≤ 7.5 h/night) had an increased risk for higher body weight in early adolescence. Similarly, children who slept ≤ 7.5 h/night had higher risk of being anxious or depressed or having learning problems in early adolescence. Citation: Silva GE; Goodwin JL; Parthasarathy S; Sherrill DL; Vana KD; Drescher AA; Quan SF. Longitudinal association between short sleep, body weight, and emotional and learning problems in Hispanic and Caucasian children. SLEEP 2011;34(9):1197-1205. PMID:21886357

  9. Detection of vancomycin-resistant enterococci (VRE in stool specimens submitted for Clostridium difficile toxin testing

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    Sevim Özsoy

    Full Text Available Abstract The aim of this study was to determine the association between Clostridium difficile (C. difficile and vancomycin-resistant Enterococcus (VRE and efficacy of screening stools submitted for C. difficile toxin assay for prevalence of VRE. Between April 2012 and February 2014, 158 stool samples submitted for C. difficile toxin to the Marmara University Microbiology Laboratory, were included in the study. Stool samples were analyzed by enzyme immuno assay test; VIDAS (bioMerieux, France for Toxin A&B. Samples were inoculated on chromID VRE (bioMerieux, France and incubated 24 h at 37 °C. Manuel tests and API20 STREP (bioMerieux, France test were used to identify the Enterococci species. After the species identification, vancomycin and teicoplanin MIC's were performed by E test and molecular resistance genes for vanA vs vanB were detected by polymerase chain reaction (PCR. Of the 158 stool samples, 88 were toxin positive. The prevalence of VRE was 17%(n:19 in toxin positives however, 11.4% in toxin negatives(n:70. All VRE isolates were identified as Enterococcus faecium. These results were evaluated according to Fischer's exact chi-square test and p value between VRE colonization and C. difficile toxin positivity was detected 0.047 (p < 0.05. PPV and NPV were 79% and 47% respectively. In our study, the presence of VRE in C. difficile toxin positives is statistically significant compared with toxin negatives (p < 0.05. Screening for VRE is both additional cost and work load for the laboratories. Therefore VRE screening among C. difficile toxin positive samples, will be cost effective for determination of high risk patients in the hospitals especially for developing countries.

  10. Resistance to Glycopeptide Antibiotics in the Teicoplanin Producer Is Mediated by van Gene Homologue Expression Directing the Synthesis of a Modified Cell Wall Peptidoglycan▿

    Science.gov (United States)

    Beltrametti, Fabrizio; Consolandi, Arianna; Carrano, Lucia; Bagatin, Francesca; Rossi, Roberta; Leoni, Livia; Zennaro, Elisabetta; Selva, Enrico; Marinelli, Flavia

    2007-01-01

    Glycopeptide resistance has been studied in detail in enterococci and staphylococci. In these microorganisms, high-level resistance is achieved by replacing the C-terminal d-alanyl-d-alanine of the nascent peptidoglycan with d-alanyl-d-lactate or d-alanyl-d-serine, thus reducing the affinities of glycopeptides for cell wall targets. Reorganization of the cell wall is directed by the expression of the van gene clusters. The identification of van gene homologs in the genomes of several glycopeptide-producing actinomycetes suggests the involvement of a similar self-resistance mechanism to avoid suicide. This report describes a comprehensive study of self-resistance in Actinoplanes teichomyceticus ATCC 31121, the producer of the clinically relevant glycopeptide teicoplanin. A. teichomyceticus ATCC 31121 showed a MIC of teicoplanin of 25 μg/ml and a MIC of vancomycin of 90 μg/ml during vegetative growth. The vanH, vanA, and vanX genes of A. teichomyceticus were found to be organized in an operon whose transcription was constitutive. Analysis of the UDP-linked peptidoglycan precursors revealed the presence of UDP-glycomuramyl pentadepsipeptide terminating in d-alanyl-d-lactate. No trace of precursors ending in d-alanyl-d-alanine was detected. Thus, the van gene complex was transcribed and expressed in the genetic background of A. teichomyceticus and conferred resistance to vancomycin and teicoplanin through the modification of cell wall biosynthesis. During teicoplanin production (maximum productivity, 70 to 80 μg/ml), the MIC of teicoplanin remained in the range of 25 to 35 μg/ml. Teicoplanin-producing cells were found to be tolerant to high concentrations of exogenously added glycopeptides, which were not bactericidal even at 5,000 μg/ml. PMID:17220405

  11. Prevalence and antimicrobial resistance pattern of multidrug-resistant enterococci isolated from clinical specimens

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    M M Salem-Bekhit

    2012-01-01

    Full Text Available Purpose: Vancomycin-resistant enterococci (VRE pose an emerging problem in hospitals worldwide. The present study was undertaken to determine the occurrence, species prevalence, antibacterial resistance, and phenotypic and genetic characteristics of VRE isolated in Riyadh hospitals, KSA. Materials and Methods: Two hundred and six isolates of enterococcal species were obtained from clinical samples. The antibiotic susceptibility of isolates and minimum inhibitory concentration (MIC tests for vancomycin and teicoplanin were determined. Molecular typing of VRE isolates was carried out by using pulsed field gel electrophoresis (PFGE and the resistance genotype was determined by polymerase chain reaction (PCR. Results: VRE accounted for 3.9% of the isolates and were detected mostly in urine, wound and blood specimens isolated from ICU, internal medicine and surgical wards. All strains were identified to species level and were found to consist of E. faecalis (69.2%, E. faecium (11.3%, E. avium (2.1%, E. hirae (0.8%, E. casseliflavus (1.3% and E. gallinarum (1.3% species. According to the susceptibility data obtained, 8 (3.9% out of 206 isolates were found to be VRE (MICs > 32 μg/ml. The vanA, vanB and vanC gene fragments of E. faecalis, E. faecium and E. gallinarum were amplified from isolates and were detected. PFGE patterns of the VRE isolates revealed homogenous patterns with dominant clone suggesting that the strains intrinsic resistance is independent. Conclusions: This study shows an emergence of VRE along with increased rate of multidrug-resistant enterococci in the area of the study. Regular surveillance of antimicrobial susceptibilities should be done regularly and the risk factors should be determined.

  12. Molecular Epidemiology of Enterococcal Bacteremia in Australia

    Science.gov (United States)

    Pearson, Julie C.; Daley, Denise A.; Le, Tam; Robinson, Owen J.; Gottlieb, Thomas; Howden, Benjamin P.; Johnson, Paul D. R.; Bennett, Catherine M.; Stinear, Timothy P.; Turnidge, John D.

    2014-01-01

    Enterococci are a major cause of health care-associated infections and account for approximately 10% of all bacteremias globally. The aim of this study was to determine the proportion of enterococcal bacteremia isolates in Australia that are antimicrobial resistant, with particular emphasis on susceptibility to ampicillin and the glycopeptides, and to characterize the molecular epidemiology of the Enterococcus faecalis and Enterococcus faecium isolates. From 1 January to 31 December 2011, 1,079 unique episodes of bacteremia were investigated, of which 95.8% were caused by either E. faecalis (61.0%) or E. faecium (34.8%). The majority of bacteremias were health care associated, and approximately one-third were polymicrobial. Ampicillin resistance was detected in 90.4% of E. faecium isolates but was not detected in E. faecalis isolates. Vancomycin nonsusceptibility was reported in 0.6% and 36.5% of E. faecalis and E. faecium isolates, respectively. Unlike Europe and the United States, where vancomycin resistance in E. faecium is predominately due to the acquisition of the vanA operon, 98.4% of E. faecium isolates harboring van genes carried the vanB operon, and 16.1% of the vanB E. faecium isolates had vancomycin MICs at or below the susceptible breakpoint of the CLSI. Although molecular typing identified 126 E. faecalis pulsed-field gel electrophoresis pulsotypes, >50% belonged to two pulsotypes that were isolated across Australia. E. faecium consisted of 73 pulsotypes from which 43 multilocus sequence types were identified. Almost 90% of the E. faecium isolates were identified as CC17 clones, of which approximately half were characterized as ST203, which was isolated Australia-wide. In conclusion, the Australian Enterococcal Sepsis Outcome Programme (AESOP) study has shown that although they are polyclonal, enterococcal bacteremias in Australia are frequently caused by ampicillin-resistant vanB E. faecium. PMID:24391201

  13. Pyrosequencing-based comparative genome analysis of the nosocomial pathogen Enterococcus faecium and identification of a large transferable pathogenicity island

    Science.gov (United States)

    2010-01-01

    Background The Gram-positive bacterium Enterococcus faecium is an important cause of nosocomial infections in immunocompromized patients. Results We present a pyrosequencing-based comparative genome analysis of seven E. faecium strains that were isolated from various sources. In the genomes of clinical isolates several antibiotic resistance genes were identified, including the vanA transposon that confers resistance to vancomycin in two strains. A functional comparison between E. faecium and the related opportunistic pathogen E. faecalis based on differences in the presence of protein families, revealed divergence in plant carbohydrate metabolic pathways and oxidative stress defense mechanisms. The E. faecium pan-genome was estimated to be essentially unlimited in size, indicating that E. faecium can efficiently acquire and incorporate exogenous DNA in its gene pool. One of the most prominent sources of genomic diversity consists of bacteriophages that have integrated in the genome. The CRISPR-Cas system, which contributes to immunity against bacteriophage infection in prokaryotes, is not present in the sequenced strains. Three sequenced isolates carry the esp gene, which is involved in urinary tract infections and biofilm formation. The esp gene is located on a large pathogenicity island (PAI), which is between 64 and 104 kb in size. Conjugation experiments showed that the entire esp PAI can be transferred horizontally and inserts in a site-specific manner. Conclusions Genes involved in environmental persistence, colonization and virulence can easily be aquired by E. faecium. This will make the development of successful treatment strategies targeted against this organism a challenge for years to come. PMID:20398277

  14. First nosocomial outbreak of vanA-type vancomycin-resistant Enterococcus raffinosus in France.

    Science.gov (United States)

    Jolivet, S; Fines-Guyon, M; Nebbad, B; Merle, J C; Le Pluart, D; Brun-Buisson, C; Decousser, J-W; Cattoir, V

    2016-12-01

    Vancomycin-resistant Enterococcus raffinosus has rarely been associated with nosocomial infection and outbreaks. To report the successful control of a nosocomial outbreak of vanA-type vancomycin-resistant E. raffinosus in a surgical intensive care unit. The investigation of the outbreak is reported with control measures taken. Molecular typing of vancomycin-resistant E. raffinosus isolates was performed by repetitive sequence-based polymerase chain reaction (PCR). Between September and October 2014, vancomycin-resistant E. raffinosus isolates were isolated from four patients. The index patient had been hospitalized previously in Portugal, and was not found to be colonized by vancomycin-resistant enterococci on screening cultures obtained at admission. However, vancomycin-resistant E. raffinosus was isolated from a bile sample 19 days after hospital admission. All four isolates were resistant to both vancomycin and teicoplanin due to the presence of the vanA gene, while remaining susceptible to daptomycin and linezolid. Repetitive sequence-based PCR confirmed the spread of a single vanA-positive E. raffinosus clone. Infection control measures including direct PCR screening on rectal specimens, contact precautions, and cohorting of patients and personnel led to successful control of the outbreak. This is the first reported outbreak of vanA-type vancomycin-resistant E. raffinosus in France in both clinical and screening specimens among hospitalized patients. The inability of routine selective screening media to detect the vancomycin-resistant E. raffinosus in the index case likely contributed to the outbreak. Copyright © 2016 The Healthcare Infection Society. Published by Elsevier Ltd. All rights reserved.

  15. Vancomycin Resistance in Staphylococcus aureus
.

    Science.gov (United States)

    McGuinness, Will A; Malachowa, Natalia; DeLeo, Frank R

    2017-06-01

    The evolution of Staphylococcus aureus during the modern antibiotic era has been delineated by distinct strain emergence events, many of which include acquisition of antibiotic resistance. The relative high burden of methicillin-resistant S. aureus (MRSA) in healthcare and community settings is a major concern worldwide. Vancomycin, a glycopeptide antibiotic that inhibits cell wall biosynthesis, remains a drug of choice for treatment of severe MRSA infections. S. aureus strains exhibiting increased resistance to vancomycin, known as vancomycin intermediate-resistant S. aureus (VISA) (MIC = 4-8 µg/mL), were discovered in the 1990s. The molecular basis of resistance in VISA is polygenic and involves stepwise mutations in genes encoding molecules predominantly involved in cell envelope biosynthesis. S. aureus isolates with complete resistance to vancomycin (MIC ≥ 16 µg/mL) are termed vancomycin-resistant S. aureus (VRSA)-they were first reported in the U.S. in 2002. Resistance in VRSA is conferred by the vanA gene and operon, which is present on a plasmid. Although treatment of VRSA infections is challenging, the total number of human VRSA infections to date is limited (14 in the U.S.). By comparison, the burden of VISA is relatively high and the molecular mechanisms of resistance are less well-defined. VISA are associated with persistent infections, vancomycin treatment failure, and poor clinical outcomes. Here, we review in brief progress made toward understanding the acquisition of antibiotic resistance in S. aureus, with an emphasis on the molecular mechanisms underlying vancomycin resistance.

  16. Within-host evolution of Enterococcus faecium during longitudinal carriage and transition to bloodstream infection in immunocompromised patients.

    Science.gov (United States)

    Moradigaravand, Danesh; Gouliouris, Theodore; Blane, Beth; Naydenova, Plamena; Ludden, Catherine; Crawley, Charles; Brown, Nicholas M; Török, M Estée; Parkhill, Julian; Peacock, Sharon J

    2017-12-27

    Enterococcus faecium is a leading cause of hospital-acquired infection, particularly in the immunocompromised. Here, we use whole genome sequencing of E. faecium to study within-host evolution and the transition from gut carriage to invasive disease. We isolated and sequenced 180 E. faecium from four immunocompromised patients who developed bloodstream infection during longitudinal surveillance of E. faecium in stool and their immediate environment. A phylogenetic tree based on single nucleotide polymorphisms (SNPs) in the core genome of the 180 isolates demonstrated several distinct clones. This was highly concordant with the population structure inferred by Bayesian methods, which contained four main BAPS (Bayesian Analysis of Population Structure) groups. The majority of isolates from each patient resided in a single group, but all four patients also carried minority populations in stool from multiple phylogenetic groups. Bloodstream isolates from each case belonged to a single BAPS group, which differed in all four patients. Analysis of 87 isolates (56 from blood) belonging to a single BAPS group that were cultured from the same patient over 54 days identified 30 SNPs in the core genome (nine intergenic, 13 non-synonymous, eight synonymous), and 250 accessory genes that were variably present. Comparison of these genetic variants in blood isolates versus those from stool or environment did not identify any variants associated with bloodstream infection. The substitution rate for these isolates was estimated to be 128 (95% confidence interval 79.82 181.77) mutations per genome per year, more than ten times higher than previous estimates for E. faecium. Within-patient variation in vancomycin resistance associated with vanA was common and could be explained by plasmid loss, or less often by transposon loss. These findings demonstrate the diversity of E. faecium carriage by individual patients and significant within-host diversity of E. faecium, but do not provide

  17. Resistência antimicrobiana em Enterococcus faecalis e Enterococcus faecium isolados de carcaças de frango

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    Ana Claudia F. Borges de Campos

    2013-05-01

    Full Text Available O objetivo deste trabalho foi realizar o isolamento e analisar o perfil de resistência antimicrobiana de Enterococcus de carcaças de frango resfriadas e congeladas comercializadas no Distrito Federal, detectando genes de resistência antimicrobiana e identificando as espécies Enterococcus faecalis e Enterococcus faecium por reação polimerase em cadeia. Foram analisadas 100 carcaças de frangos, das quais foram isoladas 50 cepas de Enterococcus spp., sendo 42% de E. faecalis e 2% de E. faecium. O teste de susceptibilidade antimicrobiana demonstrou que todas as cepas isoladas apresentaram resistência a pelo menos um antimicrobiano, dos quais 90,47% das cepas de E. faecalis, 100% das cepas de E. Faecium e 82,14% dos Enterococcus spp. apresentaram resistência à Tetraciclina; 80,95% das cepas de E. faecalis e 35,71% das cepas de Enterococcus spp. foram resistentes à Eritromicina; 39,28% dos Enterococcus spp. e 23,80% dos E. faecalis à Ciprofloxacina e 28,57% dos E. faecalis apresentaram resistência ao Cloranfenicol. Foram detectados os genes de resistência antimicrobiana erm(B, vanC-1, aph(3'-llla, ant(6-la, vanB, vanA, aac(6'-le-aph(2''-la, erm(A e tet(M - este último mais frequente. Estes resultados sugerem sérios problemas para a Saúde Pública, uma vez que esses microrganismos podem possuir a capacidade de transmitir genes de resistência antimicrobiana para outros microrganismos presentes na microbiota intestinal de humanos e animais, podendo inviabilizar o uso destas drogas para tratamentos clínicos.

  18. Chlorhexidine Induces VanA-Type Vancomycin Resistance Genes in Enterococci

    Science.gov (United States)

    Bhardwaj, Pooja; Ziegler, Elizabeth

    2016-01-01

    Chlorhexidine is a bisbiguanide antiseptic used for infection control. Vancomycin-resistant E. faecium (VREfm) is among the leading causes of hospital-acquired infections. VREfm may be exposed to chlorhexidine at supra- and subinhibitory concentrations as a result of chlorhexidine bathing and chlorhexidine-impregnated central venous catheter use. We used RNA sequencing to investigate how VREfm responds to chlorhexidine gluconate exposure. Among the 35 genes upregulated ≥10-fold after 15 min of exposure to the MIC of chlorhexidine gluconate were those encoding VanA-type vancomycin resistance (vanHAX) and those associated with reduced daptomycin susceptibility (liaXYZ). We confirmed that vanA upregulation was not strain or species specific by querying other VanA-type VRE. VanB-type genes were not induced. The vanH promoter was found to be responsive to subinhibitory chlorhexidine gluconate in VREfm, as was production of the VanX protein. Using vanH reporter experiments with Bacillus subtilis and deletion analysis in VREfm, we found that this phenomenon is VanR dependent. Deletion of vanR did not result in increased chlorhexidine susceptibility, demonstrating that vanHAX induction is not protective against chlorhexidine. As expected, VanA-type VRE is more susceptible to ceftriaxone in the presence of sub-MIC chlorhexidine. Unexpectedly, VREfm is also more susceptible to vancomycin in the presence of subinhibitory chlorhexidine, suggesting that chlorhexidine-induced gene expression changes lead to additional alterations in cell wall synthesis. We conclude that chlorhexidine induces expression of VanA-type vancomycin resistance genes and genes associated with daptomycin nonsusceptibility. Overall, our results indicate that the impacts of subinhibitory chlorhexidine exposure on hospital-associated pathogens should be further investigated in laboratory studies. PMID:26810654

  19. The current MLVA typing scheme for Enterococcus faecium is less discriminatory than MLST and PFGE for epidemic-virulent, hospital-adapted clonal types

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    Klare Ingo

    2007-04-01

    Full Text Available Abstract Background MLVA (multiple-locus variable-number tandem repeat analysis is a reliable typing technique introduced recently to differentiate also isolates of Enterococcus faecium. We used the established VNTR (variable number of tandem repeats scheme to test its suitability to differentiate 58 E. faecium isolates representing mainly outbreaks and clusters of infections and colonizations among patients from 31 German hospitals. All isolates were vancomycin-resistant (vanA type. Typing results for MLVA are compared with results of macrorestriction analysis in PFGE (pulsed-field gel electrophoresis and MLST (multi-locus sequence typing. Results All 51 but one hospital isolates from 1996–2006 were assigned to the clonal complex (CC of epidemic-virulent, hospital-adapted lineages (MLST CC-17; MLVA CC-1 and differed from isolates of sporadic infections and colonizations (n = 7; 1991–1995 and other non-hospital origins (n = 27. Typing of all 58 hospital VRE revealed MLVA as the least discriminatory method (Simpson's diversity index 0.847 when compared to MLST (0.911 and PFGE (0.976. The two most common MLVA types MT-1 (n = 16 and MT-159 (n = 14 combined isolates of several MLST types including also major epidemic, hospital-adapted, clonal types (MT-1: ST-17, ST-18, ST-280, ST-282; MT-159: ST-78, ST-192, ST-203. These data clearly indicate that non-related E. faecium could possess an identical MLVA type being especially critical when MLVA is used to elucidate supposed outbreaks with E. faecium within a single or among different hospitals. Stability of a given MLVA profile MT-12 (ST-117 during an outbreak over a period of five years was also shown. Conclusion MLVA is a suitable method to assign isolates of E. faecium into distinct clonal complexes. To investigate outbreaks the current MLVA typing scheme for E. faecium does not discriminate enough and cannot be recommended as a standard superior to PFGE.

  20. Colonization with vancomycin-resistant enterococci (VRE in intensive care unit patients in Cordoba City: Argentina Colonización con enterococos vancomicina-resistentes (EVR en una unidad de cuidados intensivos en la Ciudad de Córdoba: Argentina

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    A. M. Littvik

    2006-03-01

    Full Text Available The purpose of this study was to determine the prevalence of colonization with vancomycin-resistant enterococci (VRE among intensive care unit (ICU patients in a hospital in Córdoba, Argentina. We collected 235 rectal swab specimens from 147 ICU patients. Resistance to vancomycin was screened with the disk diffusion method, and MICs were determined with the E-test method. Vancomycin-resistant genotypes were determined by PCR. The VRE strains were isolated from 18/147 patients (12.2%. The isolates were identified as Enterococcus faecium (94.4%, and Enterococcus gallinarum (5.6%. PCR showed that the E. faecium strains carried the vanAgene, and the E. gallinarum strain carried the vanC1gene. Our study indicated that at least 12.2% of ICU patients were VRE carriers.El propósito de este estudio fue determinar la prevalencia de colonización con EVR en pacientes de una unidad de cuidados intensivos (UCI en un hospital de la Ciudad de Córdoba, Argentina. Se recolectaron 235 muestras por hisopado rectal de 147 pacientes. La resistencia a vancomicina fue estudiada por el método de difusión con discos y las CIMs fueron determinadas por E-test. Los genotipos de resistencia fueron determinados por PCR. Se aislaron cepas de EVR en 18/147 pacientes (12,2 %. Los aislamientos fueron identificados como Enterococcus faecium (94,4% y Enterococcus gallinarum (5,6%. Todas las cepas de E. faecium fueron portadoras del gen vanA y la cepa de E. gallinarum del gen vanC1 de resistencia intrínseca. Este estudio mostró que el 12,2% de los pacientes internados en la UCI fueron portadores de EVR.

  1. vanA-containing E. faecium isolates of clonal complex CC17 in clinical and environmental samples in a Tunisian hospital.

    Science.gov (United States)

    Elhani, Dalèle; Klibi, Naouel; Dziri, Raoudha; Ben Hassan, Meriem; Asli Mohamed, Selim; Ben Said, Laila; Mahjoub, Aouini; Ben Slama, Karim; Jemli, Boutheina; Bellaj, Ridha; Barguellil, Farouk; Torres, Carmen

    2014-05-01

    Twenty-eight vancomycin (VA)-resistant enterococci isolated from different patients (n = 16) and also from the environment (n = 12) were recovered in a Tunisian military hospital during 2012-2013. The mechanisms of resistance to VA and to other antibiotics as well as the presence of esp and hyl virulence genes were determined in these isolates by PCR, being their clonal relationship analyzed by pulsed-field gel electrophoresis (PFGE). VA resistance mechanisms detected were as follows (species-patient/environment): vanA (Enterococcus faecium, 13/5), vanC1 (Enterococcus gallinarum, 3/0), and vanC2 (Enterococcus casseliflavus, 0/7). Most of the VA-resistant enterococci presented a multiresistance phenotype and harbored different resistance genes (erm(B), tet(M), tet(L), ant(6)-Ia, aac(6')-aph(2"), aph(3')-IIIa, and catA). The PFGE revealed the presence of 3 clones (A, B, C) and 1 closely related pattern (A1) among the 13 vanA-containing E. faecium isolates of patients showing 11 of them the A-A1 patterns. The clone A was also detected in all 5 environmental vanA-containing E. faecium isolates. Strains did not contain esp or hyl virulence genes. Multilocus sequence typing was performed in 4 E. faecium isolates representative of the 4 detected pulsotypes (A, A1, B, and C), and 2 different sequence types were identified (ST18 and ST80), both of them included in clonal complex CC17. These strains contained the IS16 element and showed ampicillin and ciprofloxacin resistance. VA resistance could be an emerging problem in Tunisia, and this is one of the first cases described so far in this country. Copyright © 2014 Elsevier Inc. All rights reserved.

  2. Phenotypic and genotypic characterization of vancomycin-resistant Enterococcus faecium clinical isolates from two hospitals in Mexico: First detection of VanB phenotype-vanA genotype.

    Science.gov (United States)

    Bocanegra-Ibarias, Paola; Flores-Treviño, Samantha; Camacho-Ortiz, Adrián; Morfin-Otero, Rayo; Villarreal-Treviño, Licet; Llaca-Díaz, Jorge; Martínez-Landeros, Erik Alan; Rodríguez-Noriega, Eduardo; Calzada-Güereca, Andrés; Maldonado-Garza, Héctor Jesús; Garza-González, Elvira

    2016-01-01

    Enterococcus faecium has emerged as a multidrug-resistant nosocomial pathogen involved in outbreaks worldwide. Our aim was to determine the antimicrobial susceptibility, biofilm production, and clonal relatedness of vancomycin-resistant E. faecium (VREF) clinical isolates from two hospitals in Mexico. Consecutive clinical isolates (n=56) were collected in two tertiary care hospitals in Mexico from 2011 to 2014. VREF isolates were characterized by phenotypic and molecular methods including pulsed-field gel electrophoresis (PFGE). VREF isolates were highly resistant to vancomycin, erythromycin, norfloxacin, high-level streptomycin, and teicoplanin, and showed lower resistance to tetracycline, nitrofurantoin and quinupristin-dalfopristin. None of the isolates were resistant to linezolid. The vanA gene was detected in all isolates. Two VanB phenotype-vanA genotype isolates, highly resistant to vancomycin and susceptible to teicoplanin, were detected. Furthermore, 17.9% of the isolates were classified as biofilm producers, and the espfm gene was found in 98.2% of the isolates. A total of 37 distinct PFGE patterns and 6 clones (25% of the isolates as clone A, 5.4% as clone B, and 3.6% each as clone C, D, E, and F) were detected. Clone A was detected in 5 different wards of the same hospital during 14 months of surveillance. The high resistance to most antimicrobial agents and the moderate cross-transmission of VREF detected accentuates the need for continuous surveillance of E. faecium in the hospital setting. This is also the first reported incidence of the E. faecium VanB phenotype-vanA genotype in the Americas. Copyright © 2015 Elsevier España, S.L.U. and Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica. All rights reserved.

  3. Reclaimed water as a reservoir of antibiotic resistance genes: distribution system and irrigation implications

    Directory of Open Access Journals (Sweden)

    Nicole L Fahrenfeld

    2013-05-01

    Full Text Available Treated wastewater is increasingly being reused to achieve sustainable water management in arid regions. The objective of this study was to quantify the distribution of antibiotic resistance genes (ARGs in recycled water, particularly after it has passed through the distribution system, and to consider point-of-use implications for soil irrigation. Three separate reclaimed wastewater distribution systems in the western U.S. were examined. Quantitative polymerase chain reaction (qPCR was used to quantify ARGs corresponding to resistance to sulfonamides (sul1, sul2, macrolides (ermF, tetracycline (tet(A, tet(O, glycopeptides (vanA, and methicillin (mecA, in addition to genes present in waterborne pathogens Legionella pneumophila (Lmip, Escherichia coli (gadAB, and Pseudomonas aeruginosa (ecfx, gyrB. In a parallel lab study, the effect of irrigating an agricultural soil with secondary, chlorinated, or dechlorinated wastewater effluent was examined in batch microcosms. A broader range of ARGs were detected after the reclaimed water passed through the distribution systems, highlighting the importance of considering bacterial re-growth and the overall water quality at the point of use. Screening for pathogens with qPCR indicated presence of Lmip and gadAB genes, but not ecfx or gyrB. In the lab study, chlorination was observed to reduce 16S rRNA and sul2 gene copies in the wastewater effluent, while dechlorination had no apparent effect. ARGs levels did not change with time in soil slurries incubated after a single irrigation event with any of the effluents. However, when irrigated repeatedly with secondary wastewater effluent (not chlorinated or dechlorinated, elevated levels of sul1 and sul2 were observed. This study suggests that reclaimed water may be an important reservoir of ARGs, especially at the point of use, and that attention should be directed towards the fate of ARGs in irrigation water and the implications for human health.

  4. Characterization and risk factors of vancomycin-resistant Enterococci (VRE) among animal-affiliated workers in Malaysia.

    Science.gov (United States)

    Getachew, Y; Hassan, L; Zakaria, Z; Zaid, C Z M; Yardi, A; Shukor, R A; Marawin, L T; Embong, F; Aziz, S A

    2012-11-01

    This study determined the risk factors and characteristics of vancomycin-resistant Enterococci (VRE) among individuals working with animals in Malaysia. Targeted cross-sectional studies accompanied with laboratory analysis for the identification and characterization of resistance and virulence genes and with genotype of VRE were performed. VRE were detected in 9·4% (95% CI: 6·46-13·12) of the sampled populations. Enterococcus faecium, Enterococcus faecalis and Enterococcus gallinarum were isolated, and vanA was detected in 70% of the isolates. Enterococcus faecalis with vanB was obtained from one foreign poultry worker. At least one virulence gene was detected in >50% of Ent. faecium and Ent. faecalis isolates. The esp and gelE genes were common among Ent. faecium (58·3%) and Ent. faecalis (78%), respectively. The VRE species showed diverse RAPD profiles with some clustering of strains based on the individual's background. However, the risk factors found to be significantly associated with the prevalence of VRE were age (OR: 5·39, 95% CI: 1·98-14·61) and previous hospitalization (OR: 4·06, 95% CI: 1·33-12·35). VRE species isolated from individuals in this study have high level of vancomycin resistance, were genetically diverse and possessed the virulence traits. Age of individuals and history of hospitalization rather than occupational background determined VRE colonization. This study provides comprehensive findings on the epidemiological and molecular features of VRE among healthy individuals working with animals. © 2012 The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology.

  5. Investigation of biofilm formation ability, antimicrobial resistance and the staphylococcal cassette chromosome mec patterns of methicillin resistant Staphylococcus epidermidis with different sequence types isolated from children.

    Science.gov (United States)

    Soroush, Setareh; Jabalameli, Fereshteh; Taherikalani, Morovat; Amirmozafari, Nour; Fooladi, Abbas Ali Imani; Asadollahi, Khairollah; Beigverdi, Reza; Emaneini, Mohammad

    2016-04-01

    This study investigated the molecular characterizations of 80 methicillin resistant Staphylococcus epidermidis (MRSE) collected during 2012-2013 in Tehran Children's Medical Center, Iran. About 90% of MRSE isolates were multi-drug resistant (MDR) and the highest resistance was observed to cotrimoxazole and they were quite sensitive to quinupristin-dalfopristin and linezolid. Though vanA gene was not detected, the majority of isolates showed intermediate resistance to vancomycin (MIC90 16 μg/ml). Resistance to mupirocin was observed in 18 isolates. Staphylococcal cassette chromosome mec (SCCmec) types V, III, IV and II were detected in 23.75%, 7.5%, 6.25% and 5% of isolates respectively, in some of which the additional parts of mec or ccr complexes were observed. In 57.5% MRSE isolates SCCmec types were not classified. 41.2% of MRSE isolates were carrying intercellular adhesion (ica) operon and 40% had strong or intermediate biofilm. The types of arginine catabolic mobile element (ACME) were limited to type I and II. Nine sequence types (STs) were seen in mupirocin resistant MRSE isolates. The common STs were ST2, ST5 and ST22 with 27.7% (5/18), 22.2% (4/18) and 16.6% (3/18) frequencies, respectively. ST23, ST54 and ST179 plus three novels STs 580, 581,588 were also observed. The majority of STs, 83.3% (15/18) belonged to clonal complex 2 (CC2). The spread of antibiotic resistance and virulence factors among MRSE species is an alarming sign in Children's Hospitals. The combination of these two issues leads to increase the chance of successfully establishing of common STs in hospital environments, and promotes the device-related infections and bacteremia. Copyright © 2016 Elsevier Ltd. All rights reserved.

  6. Genetic relatedness and risk factor analysis of ampicillin-resistant and high-level gentamicin-resistant enterococci causing bloodstream infections in Tanzanian children.

    Science.gov (United States)

    Aamodt, Håvard; Mohn, Stein Christian; Maselle, Samuel; Manji, Karim P; Willems, Rob; Jureen, Roland; Langeland, Nina; Blomberg, Bjørn

    2015-02-28

    While enterococci resistant to multiple antimicrobials are spreading in hospitals worldwide, causing urinary tract, wound and bloodstream infections, there is little published data on these infections from Africa. We assessed the prevalence, susceptibility patterns, clinical outcome and genetic relatedness of enterococcal isolates causing bloodstream infections in children in a tertiary hospital in Tanzania, as part of a prospective cohort study of bloodstream infections among 1828 febrile children admitted consecutively from August 2001 to August 2002. Enterococcal bacteraemia was identified in 2.1% (39/1828) of admissions, and in 15.3% (39/255) of cases of culture-confirmed bloodstream infections. The case-fatality rate in children with Enterococcus faecalis septicaemia (28.6%, 4/14) was not significantly different from those with Enterococcus faecium septicaemia (6.7%, 1/15, p = 0.12). E. faecium isolates commonly had combined ampicillin-resistance and high-level gentamicin resistance (HLGR), (9/17), while E. faecalis frequently displayed HLGR (6/15), but were ampicillin susceptible. None of the tested enterococcal isolates displayed vancomycin resistance by Etest or PCR for vanA and vanB genes. Multi-locus sequence-typing (MLST) showed that the majority of E. faecium (7/12) belonged to the hospital associated Bayesian Analysis of Population Structure (BAPS) group 3-3. Pulsed-field gel electrophoresis (PFGE) indicated close genetic relationship particularly among E. faecium isolates, but also among E. faecalis isolates. There was also correlation between BAPS group and PFGE results. Risk factors for enterococcal bloodstream infection in univariate analysis were hospital-acquired infection and clinical diagnosis of sepsis with unknown focus. In multivariate analysis, neonates in general were relatively protected from enterococcal infection, while both prematurity and clinical sepsis were risk factors. Malnutrition was a risk factor for enterococcal

  7. Trends of Bloodstream Infections in a University Greek Hospital during a Three-Year Period: Incidence of Multidrug-Resistant Bacteria and Seasonality in Gram-negative Predominance.

    Science.gov (United States)

    Kolonitsiou, Fevronia; Papadimitriou-Olivgeris, Matthaios; Spiliopoulou, Anastasia; Stamouli, Vasiliki; Papakostas, Vasileios; Apostolopoulou, Eleni; Panagiotopoulos, Christos; Marangos, Markos; Anastassiou, Evangelos D; Christofidou, Myrto; Spiliopoulou, Iris

    2017-07-06

    The aim of the study was to assess the epidemiology, the incidence of multidrug-resistant bacteria and bloodstream infections' (BSIs) seasonality in a university hospital. This retrospective study was carried out in the University General Hospital of Patras, Greece, during 2011-13 y. Blood cultures from patients with clinical presentation suggestive of bloodstream infection were performed by the BacT/ALERT System. Isolates were identified by Vitek 2 Advanced Expert System. Antibiotic susceptibility testing was performed by the disk diffusion method and E-test. Resistance genes (mecA in staphylococci; vanA/vanB/vanC in enterococci; blaKPC/blaVIM/blaNDM in Klebsiella spp.) were detected by PCR. In total, 4607 (9.7%) blood cultures were positive from 47451 sets sent to Department of Microbiology, representing 1732 BSIs. Gram-negative bacteria (52.3%) were the most commonly isolated, followed by Gram-positive (39.5%), fungi (6.6%) and anaerobes bacteria (1.8%). The highest contamination rate was observed among Gram-positive bacteria (42.3%). Among 330 CNS and 150 Staphylococcus aureus, 281 (85.2%) and 60 (40.0%) were mecA-positive, respectively. From 113 enterococci, eight were vanA, two vanB and two vanC-positives. Of the total 207 carbapenem-resistant Klebsiella pneumoniae (73.4%), 202 carried blaKPC, four blaKPC and blaVIM and one blaVIM. A significant increase in monthly BSIs' incidence was shown (R2: 0.449), which may be attributed to a rise of Gram-positive BSIs (R2: 0.337). Gram-positive BSIs were less frequent in spring (P Gram-negative bacteria (P Gram-negative bacteria increased during the study period. The increasing incidence of BSIs can be attributed to an increase of Gram-positive BSI incidence, even though Gram-negative bacteria remained the predominant ones. Seasonality may play a role in the predominance of Gram-negative's BSI.

  8. Characterization of fecal vancomycin-resistant enterococci with acquired and intrinsic resistance mechanisms in wild animals, Spain.

    Science.gov (United States)

    Lozano, Carmen; Gonzalez-Barrio, David; Camacho, Maria Cruz; Lima-Barbero, Jose Francisco; de la Puente, Javier; Höfle, Ursula; Torres, Carmen

    2016-11-01

    The objectives were to evaluate the presence of vancomycin-resistant enterococci with acquired (VRE-a) and intrinsic (VRE-i) resistance mechanisms in fecal samples from different wild animals, and analyze their phenotypes and genotypes of antimicrobial resistance. A total of 348 cloacal/rectal samples from red-legged partridges (127), white storks (81), red kites (59), and wild boars (81) (June 2014/February 2015) were inoculated in Slanetz-Bartley agar supplemented with vancomycin (4 μg/mL). We investigated the susceptibility to 12 antimicrobials and the presence of 19 antimicrobial resistance and five virulence genes. In addition, we performed multilocus sequence typing, detection of IS16 and studied Tn1546 structure. One VRE-a isolate was identified in one wild boar. This isolate was identified as Enterococcus faecium, harbored vanA gene included into Tn1546 (truncated with IS1542/IS1216), and belonged to the new ST993. This isolate contained the erm(A), erm(B), tet(M), dfrG, and dfrK genes. Neither element IS16 nor the studied virulence genes were detected. Ninety-six VRE-i isolates were identified (89 Enterococcus gallinarum and seven Enterococcus casseliflavus), with the following prevalence: red kites (71.2 %), white storks (46.9 %), red-legged partridges (7.9 %), and wild boars (4.9 %). Most E. gallinarum isolates showed resistance to tetracycline (66.3 %) and/or erythromycin (46.1 %). High-level resistance to aminoglycosides was present among our VRE-i isolates: kanamycin (22.9 %), streptomycin (11.5 %), and gentamicin (9.4 %). In general, VRE-i isolates of red kites showed higher rates of resistance for non-glycopeptide agents than those of other animal species. The dissemination of acquired resistance mechanisms in natural environments could have implications in the global spread of resistance with public health implications.

  9. MicroRNA-627 mediates the epigenetic mechanisms of vitamin D to suppress proliferation of human colorectal cancer cells and growth of xenograft tumors in mice.

    Science.gov (United States)

    Padi, Sathish K R; Zhang, Qunshu; Rustum, Youcef M; Morrison, Carl; Guo, Bin

    2013-08-01

    Vitamin D protects against colorectal cancer through unclear mechanisms. We investigated the effects of calcitriol (1α,25-dihydroxyvitamin D3; the active form of vitamin D) on levels of different microRNAs (miRNAs) in colorectal cancer cells from humans and xenograft tumors in mice. Expression of miRNAs in colorectal cancer cell lines was examined using the Ambion mirVana miRNA Bioarray. The effects of calcitriol on expression of miR-627 and cell proliferation were determined by real-time polymerase chain reaction and WST-1 assay, respectively; growth of colorectal xenograft tumors was examined in nude mice. Real-time polymerase chain reaction was used to analyze levels of miR-627 in human colon adenocarcinoma samples and nontumor colon mucosa tissues (controls). In HT-29 cells, miR-627 was the only miRNA significantly up-regulated by calcitriol. Jumonji domain containing 1A (JMJD1A), which encodes a histone demethylase, was found to be a target of miR-627. By down-regulating JMJD1A, miR-627 increased methylation of histone H3K9 and suppressed expression of proliferative factors, such as growth and differentiation factor 15. Calcitriol induced expression of miR-627, which down-regulated JMJD1A and suppressed growth of xenograft tumors from HCT-116 cells in nude mice. Overexpression of miR-627 prevented proliferation of colorectal cancer cell lines in culture and growth of xenograft tumors in mice. Conversely, blocking the activity of miR-627 inhibited the tumor suppressive effects of calcitriol in cultured colorectal cancer cells and in mice. Levels of miR-627 were decreased in human colon adenocarcinoma samples compared with controls. miR-627 mediates tumor-suppressive epigenetic activities of vitamin D on colorectal cancer cells and xenograft tumors in mice. The messenger RNA that encodes the histone demethylase JMJD1A is a direct target of miR-627. Reagents designed to target JMJD1A or its messenger RNA, or increase the function of miR-627, might have the same

  10. Emergence of vancomycin resistant Staphylococcus aureus (VRSA from a tertiary care hospital from northern part of India

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    Sen Malay

    2006-10-01

    Full Text Available Abstract Background Glycopeptides such as vancomycin are frequently the antibiotics of choice for the treatment of infections caused by methicillin resistant Staphylococcus aureus (MRSA. For the last 7 years incidence of vancomycin intermediate S. aureus and vancomycin resistant S. aureus (VISA and VRSA respectively has been increasing in various parts of the world. The present study was carried out to find out the presence of VISA and VRSA in the northern part of India. Methods A total 1681 staphylococcal isolates consisting of 783 S. aureus and 898 coagulase negative staphylococci (CoNS were isolated from different clinical specimens from various outpatient departments and wards. All S. aureus and 93 CoNS were subjected to MIC testing (against vancomycin, teicolplanin and oxacillin; Brain Heart Infusion (BHI vancomycin screen agar test; disc diffusion testing, and PCR for mecA, vanA and vanB genes detection. Results Out of 783 S. aureus two S. aureus strains were found to be vancomycin and teicoplanin resistant (one strain with MIC 32 μg/ml and the other strain with MIC 64 μg/ml; six strains of S. aureus have shown to be vancomycin intermediate (two strains with MIC 16 μg/ml and four strains with MIC 8 μg/ml; and two strains with teicoplanin intermediate (MIC 16 μg/ml. One CoNS strain was resistant to vancomycin and teicoplanin (MIC 32 μg/ml, and two CoNS strains were intermediate to vancomycin and teicoplanin (MIC 16 μg/ml. All VRSA, VISA and vancomycin resistant CoNS had shown growth on BHI vancomycin screen agar (vancomycin 6 μg/ml and were mecA PCR positive. None of these isolates have demonstrated vanA/vanB gene by PCR. Conclusion The present study reveals for the first time emergence of VISA/VRSA from this part of world and indicates the magnitude of antibiotic resistance in and around the study area. The major cause of this may be unawareness and indiscriminate use of broad-spectrum antibiotics.

  11. Evaluation of the iNtRON VRE vanA/vanB real-time PCR assay for detection of vancomycin-resistant enterococci.

    Science.gov (United States)

    Huh, Hee Jae; Jang, Mi-Ae; Seo, Ja Young; Kim, Ji-Youn; Ki, Chang-Seok; Kim, Jong-Won; Lee, Nam Yong

    2015-01-01

    Recently, the iNtRON VRE vanA/vanB real-time PCR (iNtRON; iNtRON Biotechnology, Korea) assay, a multiplex real-time PCR method, was introduced. In this prospective study, we compared the iNtRON assay with the Seeplex VRE ACE detection kit (Seeplex; Seegene, Korea), a conventional multiplex PCR assay. A chromogenic agar-based culture, in which pre-selected vancomycin-resistant enterococci (VRE) was grown and subsequently plated on blood agar with vancomycin disks, was regarded as the reference method. A total of 304 consecutive rectal swab specimens were tested for VRE by culture and by iNtRON and Seeplex PCR assays. For the PCR assays, specimens were enriched for 16-24 hr before PCR. VRE were isolated from 44 (14.5%) specimens by chromogenic agar-based culture. The clinical sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of the iNtRON assay were 100% (95% confidence interval: 89.8%-100%), 99.2% (96.9%-99.9%), 95.6% (83.6%-99.2%), and 100% (98.2%-100%), respectively, while those of the Seeplex assay were 97.7% (86.2%-99.9%), 99.6% (97.5%-99.9%), 97.7% (86.2%-99.9%), and 99.6% (97.5%-99.9%), respectively. The iNtRON assay had a detection limit of 3,159 copies/µL and 13,702 copies/µL for the vanA and vanB genes, respectively. No cross-reactivity was observed in 11 non-VRE bacterial culture isolates. The overall performance of the iNtRON assay was comparable to that of a chromogenic agar-based culture method for prompt identification of VRE-colonized patients in hospitals. This assay could be an alternative or supportive method for the effective control of nosocomial VRE infection.

  12. Antibiotic resistance of Lactobacillus pentosus and Leuconostoc pseudomesenteroides isolated from naturally-fermented Aloreña table olives throughout fermentation process.

    Science.gov (United States)

    Casado Muñoz, María del Carmen; Benomar, Nabil; Lerma, Leyre Lavilla; Gálvez, Antonio; Abriouel, Hikmate

    2014-02-17

    of the present study demonstrate that all Lb. pentosus and Lc. pseudomesenteroides strains lack transferable resistance-related genes (cat, bla, blaZ, ermA, ermB, ermC, msrA/B, ereA, ereB, mphA, mefA, tet(M), tet(O), tet(S), tet(W), tet(L), tet(K), aad(E), aac(6')-Ie-aph(2')-Ia, aph(2')-Ib, aph(2')-Ic, aph(2')-Id, aph(3')-IIIa, ant(4')-Ia, dfrA, dfrD, vanA, vanB, vanC and vanE) and should therefore, according to Qualified Presumption of Safety criteria, be considered safe for future application as starter cultures or as probiotics. Copyright © 2013 Elsevier B.V. All rights reserved.

  13. Lluvia de semillas y emergencia de plántulas de Fagus grandifolia subsp. mexicana en La Mojonera, Hidalgo, México Seed rain and seedling emergence of Fagus grandifolia subsp. mexicana at La Mojonera, Hidalgo, Mexico

    Directory of Open Access Journals (Sweden)

    Oliva Godínez-Ibarra

    2007-06-01

    Full Text Available Fagus grandifolia subsp. mexicana es una especie restringida a pequeñas poblaciones y sujeta a fuerte presión antropogénica. Con el objetivo de obtener información relevante que permita proponer alternativas de conservación, se analizó la producción de semillas y la demografía de plántulas de primer año en la Mojonera, Hidalgo. Se estableció una parcela de observación de 4 800 m² dividida en cuadrantes de 10 X 10 m. Se utilizaron trampas de 0.5 m² para estimar la producción de semillas, así como subparcelas de 1 m² para registrar la emergencia y supervivencia de plántulas. La producción fue de 521 667 semillas ha-1, de las que sólo el 24.44% estaban llenas; el 46.01% vanas, y el 29.55% dañadas. La densidad de plántulas emergidas varió de 1 a 33 plántulas por m². El porcentaje de supervivencia de plántulas de primer año fue de 2.8% después de 10 meses de observación, siendo las de mayor supervivencia las que emergieron durante las primeras fechas. El 34.44% de las plántulas murieron por herbivoría, el 24.07% por damping-off y 23.65% por causa desconocida. La especie presenta el patrón general de supervivencia de especies arbóreas con alta mortalidad durante el primer año de vida.Fagus grandifolia subsp. mexicana is a species restricted to small populations under high anthropogenic pressure. With the aim to attain information to propose conservation strategies of this species, the seed rain and demography of current-year seedlings were analyzed at La Mojonera, Hidalgo, Mexico. A 4 800 m² plot divided into 10 X 10 m quadrants was established. The seed rain, seedling emergence, and survival were analyzed using seed traps of 0.5 m² and adjacent 1 m² sub-plots. The total seed rain was 521 667 seeds ha-1. A high proportion of seeds were unsound (46.01%, followed by damaged seeds (29.5% and only 24.44% were sound. Emerged seedlings fluctuated from 1 to 33 seedlings m². Alter 10 months, 2.8% of emerged seedlings were

  14. CRUZAMIENTOS INTRA E INTERESPECIFICOS EN TURNERA , SERIE CANALIGERAE

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    María Mercedes Arbo

    1987-01-01

    Full Text Available Canaligerae es una de las 9 series del género Turnera. Las especies de esta serie tienen la morfología floral más avanzada, y son la única serie conocida hasta ahora con número básico x = 5. Un programa de cruzamientos controlados se llevó a cabo entre  13 especies, cuatro de ellos con citotipos diploides y tetraploides,totalizando 17 accesiones de las regiones tropicales y subtropicales de América. La producción de híbridos se utilizó para determinar el grado de afinidad entre las entidades. La producción de los híbridos no viables, buenas semillas que no germinan, semillas vanas y el éxito de los cruces no fueron considerados como fracasos Sólo la producción de híbridos viables fue consideradas como éxitos. De las 136 combinaciones posibles (contando cada cruce y su recíproco a ser una cruz 53 híbridos y fracasos 83 fueron recuperados. La capacidad de cruzamiento entre las especies diploides indica que las especies con flores de color azul-blanca (T.caerulea, T.sunnamensis y T.grandiflora son un grupo estrechamente relacionado, y lo mismo ocurre con las especies de flores amarillas (T.scabra, T.subulata, T. Krapovickasii y T.concinna. Las especies con flores de color azul-blanco son genéticamente aisladas no sólo de las especies con flores amarillas, sino también del resto de las especies estudiadas aquí. T.coriacea y T.hermannioides parecen estar también genéticamente aisladas del resto de las especies consideradas aquí. T.aff.coriacea y T.hermannioides parecen también estar genéticamente aisladas del resto de las especies consideradas aquí. Por otro lado, los híbridos intraespecíficos entre citotipos señala la continuidad genética entre los individuos 2x y 4x que pertenecen a la misma especie. Alopolyploids (T.grandidentata, T.orientalis, T.ulmifolia y T. Aurelii tener una gama más amplia de cruzabilidad probablemente debido a la presencia de genomas diferentes en sus cromosomas

  15. Molecular characterization of antibiotic-resistant Staphylococcus aureus from livestock (bovine and swine

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    Asima Zehra

    2017-06-01

    Full Text Available Aim: The aim of this study was to figure the prevalence, phenotypic and genotypic antibiotic resistance (AR pattern of Staphylococcus aureus isolated from bovine and swine nares. Materials and Methods: Colonies with typical morphology on Baird-Parker agar supplemented with egg-yolk tellurite emulsion were selected and biochemically/genotypically identified as S. aureus. These strains were further subjected to epsilometer test for their sensitivity to various clinically important antibiotics and antibiotic susceptibility testing for amoxicillin/clavulanic acid, and double-disk diffusion testing was performed by the standard disc diffusion method following CLSI guidelines. S. aureus strains were also tested for the presence of AR genes, viz., blaZ, mecA, aacA-aphD, erm (ermA, ermB, ermC, tet (efflux genes tetK and tetL, tetM and tetO of the ribosomal protection family, and vanA. Results: The nasal cavities of 17 out of 47 randomly selected bovine and 20 out of 28 randomly selected swine were positive for S. aureus, representing the prevalence of 36.2% (95% confidence interval [CI]: 22.5-49.9 and 71.4% (95% CI: 54.7-88.1, respectively. Most of the S. aureus strains showed higher resistance to penicillin (94.6%, minimal inhibitory concentration [MIC] =1.5 μg/ml followed by ciprofloxacin (56.7%, MIC =32 μg/ml and tetracycline (18.9%, MIC =32 μg/ml. About 10-15% of the strains were resistant to gentamicin (MIC 16 μg/ml and oxacillin (MIC 6-8 μg/ml. None of the strains were resistant to vancomycin (MIC 0.25-1.5 μg/ml. In this study, 32.4% strains were resistant to three or more than three antibiotics and prevalence of this multi-drug resistant S. aureus was 45% (95% CI: 26.6-63.4 and 17.6% (95% CI: 6.7- 28.5 in swine and bovine nasal samples, respectively. Four strains from pigs were borderline oxacillin-resistant S. aureus MIC 6-8 μg/ml, but none were mecA positive. Two of these strains were β-lactamase hyperproducers. Among the resistance

  16. Respuesta del girasol a diferentes profundidades de incorporación del fertilizante Response of sunflower to deep fertilization

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    Peña C. Jorge

    1989-12-01

    Full Text Available El estudio se realizó con el objetivo de analizar la respuesta del girasol a la fertilización a diferentes profundidades. Los tratamientos fueron: incorporación del fertilizante a 10 cm con rastrillo pulidor, a 15 cm con rastra-arado, a 25 cm con arado de discos y a 30 cm con cincel-abonador; testigo absoluto sin fertilizar. Se presentaron incrementos en rendimiento a la incorporación del fertilizante a 30 cm, mostrando diferencias altamente significativas con la fertilización a 10 cm y el testigo, los incrementos en rendimiento fueron de 64.9% y 109.7% en promedio para cada uno de los semestres. Igualmente, se observaron aumentos en el diámetro del capítulo, en el peso de 1.000 semillas, en la altura de planta y disminución en el porcentaje de semillas vanas en el capitulo. Los porcentajes de cáscara, almendra, aceite y proteína no respondieron a la fertilización profunda. Desde el punto de vista económico, la mejor alternativa correspondió a la fertilización a 30 cm con cincel-abonador; la incorporación a 15 cm, con la rastra-arado, se presentó como según da opción con una tasa de retorno marginal aceptable, la cual para la región se estimó en 100 % como valor mínimo.The objective of the study was to analize the response in yield and other agronomic characteristics of sunflower based on different depths of fertilizer application. The treatments were application of fertilizer 10 cm depth tandem disk harrow, 15 cm depth with disk harrow, 25 cm depth with disk plow and 30 cm depth with chisel; an absolut control was also included. The incorporation with chisel was significantly higher than disk harrow and the control, but not with tandem disk harrow and disk plow. Increments of the chisel treatment with respect to disk harrow for the two consecutive semester were thus: for yield 64.9% and 109.7%; for capitulum diameter 20.1% and 29.9%: for weight of 1.000 seeds 34.7% and 37.1 % for plant height 15.6 % and 27.7 %, and a

  17. "Kes kaunis on, peab andma vastse võsu..." : [sonetid] / William Shakespeare ; tlk. Harald Rajamets, Ants Oras

    Index Scriptorium Estoniae

    Shakespeare, William, 1564-1616

    2006-01-01

    Sisu: "Kes kaunis on, peab andma vastse võsu..." ; "Sa peeglist näed: need palgejooned nüüd..." ; "Seesama aeg, mis kunstipeenelt voolib..." ; "Miks muusika teeb nukraks sind, kes ise..." ; "Ei, armastust ei tunne sinu rind..." ; "Nii ruttu, kui sa hääbud, uueks sirgud..." ; "Kui kuulen, kuidas aega mõõdab kell..." ; "Kuid miks sa ise tõhusamal moel..." ; "Kas suvepäeva sarnaseks sind kiita?..." ; "Aeg, õgard, küüned lõvil tömbiks kääna... ; "Ei veena peegel mind, et olen vana..." ; "Just nagu näitleja, kel kinni keel..." ; "Mu silm kui taidur võttis pilti teha..." ; "Mu armu isand, kelle truu vasall..." ; "Ma reisist väsinuna heidan sängi..." ; "Kui õnnest hüljatuna nutan sala..." ; "Kui vaikse leebe mõtte kohtu ette..." ; "Ma olen näinud küll, kuis tõusev päike..." ; "Ma tunnistan, et meid on siiski kaks..." ; "Nii nagu isa, vanadusest nõrka..." ; "Kui sulen laud, mu silmad hästi näevad..." ; "Nii ränk on reisida mul tüütul teel..." ; "Mis on su olemus, mis tuum su, miks..." ; "Ei kuningate uhked marmorsambad..." ; "Arm, uuenda end, nii et öelda sust..." ; "Kui uut ei olegi, vaid kõik on sama..." ; "Nii nagu lained ulgumerelt randa..." ; "Neiks päeviks, kui kord aeg mu armsal palge..." ; "Et näen, kuis aja käsi kõike lõhub..." ; "Kui pronksi, kivi, maad ja merd võib neelda..." ; "Mu tusk on ränk, nüüd surma kutsuks küll..." ; "Kui suren, ära leina mind sa kaua..." ; "Sa minus seda aastaaega näed..." ; "Mul sinust kirjutades arg on meel..." ; "Oo hüvasti! Liig kallis mul su hind..." ; "Siis vihka mind, kui pead, ja kohe praegu..." ; "Mõnd uhkeks teeb ta kunst, mõnd sünnipära..." ; "Sust eemal olin kevadel, mu arm..." ; "Kui möödaläinud aja kroonikais..." ; "Mis on veel ajul paberile kanda..." ; "Jah, tõsi, olen käinus siin ja seal..." ; "Ma uskuda ei saa, et murda suudab..." ; "On parem olla halb kui halvaks peetud..." ; "Ei, aeg, sa ära hoople, et mind muudad..." ; "Mu muusika, kui muusikat sa teed

  18. Cúpula Metálica en Vitoria - España

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    Buxade, C.

    1976-05-01

    Full Text Available This self-supporting dome, in the shape of a spheric cap and with a 5.850 m2 surface, rests upon 36 concrete pillars, placed at an equal distance from each other in a circle with a diameter of 76 m and a corbeling of another 3 m. The clear height between the horizontal level of the supports up to the centre of the dome is 15 m and the total weight of the structure is 102 t. The dome is defined by a two layer tetrahedral grid framework. The supporting nodes belong to the inner layer which has a variable depth and it diminishes towards the crown as the loads it has to support become smaller. This is a logical result of the adopted triangulation which is made possible by the construction with sucessive rings. Consequently the assembly has been simplified and the construction time has been reduced. The assembly has been done constructing the first two rings in their final position and the rest of the dome in two parts. These were constructed on the ground and were then lifted by means of telescopic crane. The structure has been designed to stand a load of 120 kp/m2 and was calculated by means of the assimilation method. This method determines the elastic constants of a continuous shell, with the same dimensions, shape and boundary conditions as those of the real dome, assuming the same deflections. From these forces in the ficticious assimilated dome, the tensile and compressive forces in the real grid framework can be worked out.Esta cúpula autoportante, con forma de casquete esférico de 5.850 m2 de superficie, apoya sobre 36 pilares de hormigón equidistantes y dispuestos en vana, circunferencia de 76 m de diámetro, con vuelo de otros 3 m. La altura libre desde el plano horizontal de los apoyos hasta la clave es de 15 m, siendo el peso total de la estructura de 102 t. La cúpula está definida, desde su arranque, por una malla tetraédrica de dos capas, perteneciendo los nudos de apoyo a la capa interior, cuyo canto es variable

  19. Prevalence and Antimicrobial Resistance of Enterococcus Species: A Hospital-Based Study in China

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    Wei Jia

    2014-03-01

    exhibited various resistances to the same antimicrobial agent, while reserpine treatment reduced the resistance of Enterococcus species to ciprofloxacin, gatifloxacin and levofloxacin. The β-lactamase gene TEM, aminoglycoside-modifying-enzyme genes aac(6'-aph(2", aph(3'-III, ant(6-I and ant(2"-I, tetracycline resistance gene tetM, erythromycin resistance gene ermB, vancomycin resistance gene vanA and the enterococcal multidrug resistance efflux emeA gene were detected in 77%, 62%, 26%, 13%, 36%, 31%, 66%, 5% and 55% of the 100 multiple-drug resistant enterococcal isolates. Conclusions: similar to previous findings, E. faecium and E. faecalis are predominant conditionally pathogenic bacteria that cause hospital-acquired infections that can cause urinary and respiratory system infections. Multiple and high-level antimicrobial resistance is highly prevalent in the hospital isolates of Enterococcus species. Reserpine treatment inhibits the active efflux of Enterococcus species to ciprofloxacin, gatifloxacin and levofloxacin in vitro and reduces the MIC of Enterococcus species to these three fluoroquinolones. The presence of the enterococcal multidrug resistance efflux emeA gene is associated with the resistance to antibiotics in Enterococcus species. The monitoring of the prevalence and antimicrobial resistance of Enterococcus species is of great significance to guide the control and prevention of enterococcal infections.

  20. Trends in antimicrobial resistance among clinical isolates of enterococci in a Brazilian tertiary hospital: a 4-year study Evolução da resistência aos antimicrobianos entre isolados clínicos de enterococos em um hospital terciário brasileiro: um estudo de 4 anos

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    Natália Conceição

    2011-04-01

    Full Text Available INTRODUCTION: In the past two decades members of the genus Enterococcus have emerged as important nosocomial pathogens worldwide. This study prospectively analyzed the distribution of species and trends in antimicrobial resistance among clinical isolates of enterococci in a Brazilian tertiary hospital from 2006-2009. METHODS: Enterococcal species were identified by conventional biochemical tests. The antimicrobial susceptibility profile was performed by disk diffusion in accordance with the Clinical and Laboratory Standards Institute (CLSI. A screening test for vancomycin was also performed. Minimal inhibitory concentration (MIC for vancomycin was determined using the broth dilution method. Molecular assays were used to confirm speciation and genotype of vancomycin-resistant enterococci (VRE. RESULTS: A total of 324 non-repetitive enterococcal isolates were recovered, of which 87% were E. faecalis and 10.8% E. faecium. The incidence of E. faecium per 1,000 admissions increased significantly (p 256µg/ mL and harbored vanA genes. The majority (89.5% of VRE belonged to E. faecium species, which were characteristically resistant to ampicillin and quinolones. Overall, ampicillin resistance rate increased significantly from 2.5% to 21.4% from 2006-2009. Resistance rates for gentamicin, chloramphenicol, tetracycline, and erythromycin significantly decreased over time, although they remained high. Quinolones resistance rates were high and did not change significantly over time. CONCLUSIONS: The data obtained show a significant increasing trend in the incidence of E. faecium resistant to ampicillin and vancomycin.INTRODUÇÃO: Nas últimas duas décadas, os enterococos emergiram como importantes patógenos nosocomiais no mundo inteiro. Neste estudo, foi analisada a distribuição das espécies e a evolução da resistência aos antimicrobianos entre isolados clínicos de enterococos obtidos em um hospital terciário, no período de 2006 a 2009. M