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Sample records for vai tr ca

  1. Validation of the Turkish Version of the Montreal Cognitive Assessment Scale (MoCA-TR) in patients with Parkinson's disease.

    Science.gov (United States)

    Ozdilek, Betul; Kenangil, Gulay

    2014-01-01

    The study aimed to examine the reliability and validity of the Turkish version of the Montreal Cognitive Assessment Scale (MoCA-TR) as a screening tool for cognitive dysfunction in Parkinson's disease (PD). A total of 50 patients with PD and 50 healthy controls were included. The screening instruments-MoCA-TR followed by the Mini-Mental Status Examination (MMSE-TR) and MoCA-TR retest within 1 month-and detailed neuropsychological testing were administered to the PD patients. MoCA-TR and MMSE-TR were also administered to controls. The discriminant validities of the MoCA-TR and MMSE-TR as screening and diagnostic instruments were ascertained. The concurrent and criterion validity, test-retest reliability, and internal consistency of the MoCA-TR and MMSE-TR were examined. The Cronbach's alpha of the MoCA-TR as an index of internal consistency was 0.664, and the test-retest reliability of MoCA-TR was 0.742. With a cut-off score of TR showed sensitivity of 59% and specificity of 89% in the detection of cognitive dysfunction in PD. The area under the receiver-operating characteristics curve (95% confidence interval) for MoCA-TR was 0.794 (0.670-0.918), pTR has acceptable psychometric properties and it should be used to assess mild cognitive impairment and early dementia in PD patients, whereas the MMSE-TR should remain the instrument of choice to assess cognitive impairment in PD dementia.

  2. Synthesis and structural characterization of the Zintl phases Na{sub 3}Ca{sub 3}TrPn{sub 4}, Na{sub 3}Sr{sub 3}TrPn{sub 4}, and Na{sub 3}Eu{sub 3}TrPn{sub 4} (Tr=Al, Ga, In; Pn=P, As, Sb)

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Yi [Department of Chemistry & Biochemistry, University of Delaware, 304A Drake Hall, Newark, DE 19716 (United States); Suen, Nian-Tzu [Department of Chemistry & Biochemistry, University of Delaware, 304A Drake Hall, Newark, DE 19716 (United States); College of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002 (China); Kunene, Thabiso; Stoyko, Stanislav [Department of Chemistry & Biochemistry, University of Delaware, 304A Drake Hall, Newark, DE 19716 (United States); Bobev, Svilen, E-mail: bobev@udel.edu [Department of Chemistry & Biochemistry, University of Delaware, 304A Drake Hall, Newark, DE 19716 (United States)

    2017-05-15

    15 new quaternary Zintl phases have been synthesized by solid-state reactions from the respective elements, and their structures have been determined by single-crystal X-ray diffraction. Na{sub 3}E{sub 3}TrPn{sub 4} (E=Ca, Sr, Eu; Tr=Al, Ga, In; Pn=P, As, Sb) crystallize in the hexagonal crystal system with the non-centrosymmetric space group P6{sub 3}mc (No. 186). The structure represents a variant of the K{sub 6}HgS{sub 4} structure type (Pearson index hP22) and features [TrPn{sub 4}]{sup 9–} tetrahedral units, surrounded by Na{sup +} and Ca{sup 2+}, Sr{sup 2+}, Eu{sup 2+} cations. The nominal formula rationalization [Na{sup +}]{sub 3}[E{sup 2+}]{sub 3}[TrPn{sub 4}]{sup 9–} follows the octet rule, suggesting closed-shell configurations for all atoms and intrinsic semiconducting behavior. However, structure refinements for several members hint at disorder and mixing of cations that potentially counteract the optimal valence electron count. - Graphical abstract: The hexagonal, non-centrosymmetric structure of Na{sub 3}E{sub 3}TrPn{sub 4} (E=Ca, Sr, Eu; Tr=Al, Ga, In; Pn=P, As, Sb) features [TrPn{sub 4}]{sup 9–} tetrahedral units, surrounded by Na{sup +} and Ca{sup 2+}, Sr{sup 2+}, Eu{sup 2+} cations. - Highlights: • 15 quaternary phosphides, arsenides, and antimonides are synthesized and structurally characterized. • The structure is a variant of the hexagonal K{sub 6}HgS{sub 4}-type, with distinctive pattern for the cations. • Occupational and/or positional disorder of yet unknown origin exists for some members of the series.

  3. Magnesium-Dependent Interaction of PKR with Adenovirus VAI

    Energy Technology Data Exchange (ETDEWEB)

    K Launer -Felty; C Wong; A Wahid; G Conn; J Cole

    2011-12-31

    Protein kinase R (PKR) is an interferon-induced kinase that plays a pivotal role in the innate immunity pathway for defense against viral infection. PKR is activated to undergo autophosphorylation upon binding to RNAs that contain duplex regions. Activated PKR phosphorylates the {alpha}-subunit of eukaryotic initiation factor 2, thereby inhibiting protein synthesis in virus-infected cells. Viruses have evolved diverse PKR-inhibitory strategies to evade the antiviral response. Adenovirus encodes virus-associated RNA I (VAI), a highly structured RNA inhibitor that binds PKR but fails to activate. We have characterized the stoichiometry and affinity of PKR binding to define the mechanism of PKR inhibition by VAI. Sedimentation velocity and isothermal titration calorimetry measurements indicate that PKR interactions with VAI are modulated by Mg{sup 2+}. Two PKR monomers bind in the absence of Mg{sup 2+}, but a single monomer binds in the presence of divalent ion. Known RNA activators of PKR are capable of binding multiple PKR monomers to allow the kinase domains to come into close proximity and thus enhance dimerization. We propose that VAI acts as an inhibitor of PKR because it binds and sequesters a single PKR in the presence of divalent cation.

  4. Interjectia vai in limba romana vorbita

    DEFF Research Database (Denmark)

    Sauciuc, Gabriela-Alina

    2003-01-01

    ). Schiţă de tipologie) are analysed, using conversation analysis methods and concepts from pragmatics and cognitive linguistics. The interjection is found to be predominant in female speech, and in interactions involving story telling and evaluation. The sequential distribution being relevant...... various types of shifts: in the participation framework, mental spaces, stance, from objective to subjective discourse etc. To my knowledge, no other detailed study or analysis of the Romanian interjection vai, based on authentic language data, has been attempted before. Along with the rest...

  5. Generative Graph Grammar of Neo-Vaiśeṣika Formal Ontology (NVFO)

    Science.gov (United States)

    Tavva, Rajesh; Singh, Navjyoti

    NLP applications for Sanskrit so far work within computational paradigm of string grammars. However, to compute 'meanings', as in traditional śā bdabodha prakriyā-s, there is a need to develop suitable graph grammars. Ontological structures are fundamentally graphs. We work within the formal framework of Neo-Vaiśeṣika Formal Ontology (NVFO) to propose a generative graph grammar. The proposed formal grammar only produces well-formed graphs that can be readily interpreted in accordance with Vaiśeṣ ika Ontology. We show that graphs not permitted by Vaiśeṣ ika ontology are not generated by the proposed grammar. Further, we write Interpreter of these graphical structures. This creates computational environment which can be deployed for writing computational applications of Vaiśeṣ ika ontology. We illustrate how this environment can be used to create applications like computing śā bdabodha of sentences.

  6. Reliability and Validity of the Valued Activity Inventory for Adults with Cancer (VAI-AC)

    Science.gov (United States)

    Lyons, Kathleen Doyle; Hegel, Mark T.; Hull, Jay G.; Li, Zhongze; Balan, Stefan; Bartels, Stephen

    2011-01-01

    Objective To assess the psychometric properties of the Valued Activity Inventory for Adults with Cancer (VAI-AC), a self-report instrument measuring activity limitations. Participants Fifty older adults undergoing chemotherapy. Methods Participants completed the VAI-AC and measures of physical and mental function, symptom intensity, and mood three days before and on the day of chemotherapy. Test-retest reliability was assessed by determining the average number of items for which the importance of an activity was rated consistently and by calculating the intraclass correlation coefficient (ICC) for the first and second VAI-AC scores. Convergent validity was assessed by correlating the VAI-AC scores with the other measures. Results Participants consistently rated the importance of 90% of the items. Seventy-two hour test-retest reliability was ICC = 0.67. Participants with fewer activity limitations indicated better physical function (r = 0.58, p< 0.001), better mental function (r = 0.55, p< 0.001), lower symptom intensity (r = −0.57, p< 0.001), and fewer depressive symptoms (r = −0.68, p< 0.001). Conclusion The VAI-AC demonstrated evidence of test-retest reliability and convergent validity in this convenience sample of older adults undergoing chemotherapy for cancer. PMID:22826691

  7. Ecologia trófica e reprodução do cação-frango Rhizoprionodon Ialandii (Elasmobranchii, Carcharhinidae), capturado no Sul do Brasil

    OpenAIRE

    Bornatowski, Hugo

    2010-01-01

    Resumo: O cação-frango Rhizoprionodon lalandii é um dos principais elasmobrânquios capturados por pescarias artesanais ao longo da costa brasileira. Entre junho/2006 e julho/ 2007 foram observados desembarques pesqueiros em uma comunidade pesqueira artesanal do balneário de Ipanema, costa central do Paraná. Exemplares do cação-frango Rhizoprionodon lalandii foram obtidos para estudos de limentação e reprodução. Esta espécie apresentou uma dieta generalista, consumindo presas pelágicas e deme...

  8. E o Oscar vai para... o México

    Directory of Open Access Journals (Sweden)

    Jeferson Ferro

    2016-06-01

    Full Text Available Nas últimas três edições da premiação mais popular da indústria cinematográfica, o Oscar, três profissionais mexicanos foram colocados em evidência: os diretores Alfonso Cuarón e Alejandro Iñarritu, e o diretor de fotografia Emmanuel Lubezki. Esta ascensão mexicana coincide com um momento político em que o discurso xenófobo se intensifica nos EUA, ao mesmo tempo em que a indústria cinematográfica se vê ameaçada pela TV e internet, que vêm cada vez mais conquistando audiência, reconhecimento artístico e dinheiro no mercado da ficção audiovisual. Estaríamos diante de um momento de crise em Hollywood? Propomos aqui uma breve reflexão sobre os dois últimos filmes de Iñarritu, Birdman e O Regresso, dentro deste contexto de turbulência que envolve a indústria cinematográfica norte-americana, buscando identificar algumas características no seu trabalho que o destacam no cenário atual. Palavras-chave: Iñarritu; Birdman; O Regresso; Oscar; Lubezki.DOI: http://dx.doi.org/10.21882/ruc.v4i6.631 And the Oscar goes to... Mexico!ABSTRACTIn the last three editions of the most popular prize of the cinema industry, the Oscars, three Mexican professionals stood out: directors Alfonso Cuarón and Alejandro Iñarritu, and cinematographer Emmanuel Lubezki. This Mexican uprising coincides with a turbulent moment in USA’s politics in which the xenophobic discourse is intensified, at the same time the cinema industry feels threatened by the growth in importance of internet and TV, which have been gaining artistic relevance and revenues in the audiovisual market. Does it mean that Hollywood is facing a critical moment? In this paper we look at Iñarritu’s two last films, Birdman and The Revenant, under the light of some issues that involve the cinema industry in North America today, as we try to highlight specific characteristics of his work that make it stand out in this scenario. Key-words: Iñarritu, Birdman, The Revenant, Oscar

  9. Discriminatory Ability of Visceral Adiposity Index (VAI) in Diagnosis of Metabolic Syndrome: A Population Based Study.

    Science.gov (United States)

    Motamed, N; Khonsari, M R; Rabiee, B; Ajdarkosh, H; Hemasi, G R; Sohrabi, M R; Maadi, M; Zamani, F

    2017-03-01

    Background Visceral adiposity index (VAI) has been suggested as an index of visceral adiposity. This study was conducted to determine the discriminatory ability of VAI in diagnosis of metabolic syndrome (MetS). Methods and materials We used the data of 5 312 subjects aged 18-74 years of a cohort study conducted among 6 140 individuals aged 10-90 years in Amol, northern Iran. The city population was divided into 16 strata based on gender and age groups in 10-year intervals. The subjects were randomly selected from each stratum. MetS was defined based on National Cholesterol Education Program Adult Treatment Panel III (NCEP/ATPIII), American Heart Association/National Heart, Lung and Blood Institute (AHA/NHLBI) update of Adult Treatment Panel III (ATPIII), International Diabetes Federation (IDF) and joint interim statement (JIS) definitions. The discriminatory ability of VAI and other obesity measures were evaluated using receiver operating characteristic (ROC) curves. Results While waist circumference (WC) showed the highest discriminatory ability for MetS in IDF definition in men (AUC=0.899 [CI=0.888-0.910]), VAI had the greatest discriminatory ability according to other definitions in men and women. The related AUCs of VAI were 0.866 (95%CI: 0.850-0.881), 0.829 (95%CI: 0.813-0.846), 0.859 (95%CI: 0.844-0.873) and 0.876 (95%CI: 0.863-0.889) based on NCEP/ATPIII, AHA/NHLBI update of ATPIII, IDF and JIS definition in men, and also 0.888 (95%CI: 0.875-0.902), 0.894 (95%CI: 0.881-0.907), 0.883 (95%CI: 0.869-0.897) and 0.879 (95%CI: 0.864-0.894) in women, respectively. Conclusion VAI showed an excellent discriminatory ability in diagnosis of MetS. Considering its relatively simple calculation, this index could be suggested as a reliable tool in medical practice. © Georg Thieme Verlag KG Stuttgart · New York.

  10. Fra TR til HR?

    DEFF Research Database (Denmark)

    Hagedorn-Rasmussen, Peter; Gleerup, Janne; Bojesen, Anders

    2013-01-01

    Introduktion til temanummer 'Fra TR til HR? Arbejdsmiljøets rolle i moderne arbejdspladsudvikling'......Introduktion til temanummer 'Fra TR til HR? Arbejdsmiljøets rolle i moderne arbejdspladsudvikling'...

  11. Ketterät menetelmät ja CMMI: yhteensopivia vai -sopimattomia?

    OpenAIRE

    Kuhno, Hanna

    2009-01-01

      Kuhno, Hanna Maria Ketterät menetelmät ja CMMI: yhteensopivia vai -sopimattomia?/Hanna Kuhno Jyväskylä: Jyväskylän yliopisto, 2009 47 s. Kandidaatintutkielma Tässä tutkielmassa tutustutaan ketterien menetelmien soveltamiseen CMMI (Capability Maturity Model Integration) nimisen prosessien kypsyystasomallin yhteydessä. Tavoitteena on aihealueeseen tutustumisen lisäksi selvittää CMMI:n ja ketterien menetelmien yleisimmät yhteensopivuusongelmat sekä tuoda esille myös ...

  12. Impacto del tráfico de equipos durante la cosecha de caña de azúcar (Saccharum officinarum Impact of traffic equipment during sugarcane (Saccharum officinarum harvest

    Directory of Open Access Journals (Sweden)

    Luís A. Rodríguez

    2012-10-01

    Full Text Available Para determinar el impacto del tráfico sobre el suelo, el cultivo y el consumo energético durante la cosecha de caña de azúcar en el valle del río Cauca (Colombia, se establecieron experimentos de cuatro repeticiones con diferentes sistemas de cosecha. En cada sitio se cosecharon mecánicamente parcelas con vagones de auto volteo, HD8000, HD12000 y HD20000, se evaluaron por la intensidad de tráfico (IT, el pisoteo directo sobre la cepa, la resistencia a la penetración y el consumo energético. Vagones grandes y pesados causaron mayor IT y mayor efecto por compactación y pisoteo. La IT varió entre 241 y 317 Mg km ha-1. El pisoteo en la cabecera varió de 8 a 18 m por surco y sobre la cepa los vagones pisaron entre 5 y 24% de su ancho. Hubo diferencias no significativas en productividad hasta de 13,9% favorable a los vagones livianos. En cosecha semimecánica, realizada con trenes de vagones, disminuyó la IT al rango 60-113 Mg km ha-1; pero aumentó el pisoteo en las cabeceras hasta 39 m por surco, hubo diferencias no significativas en productividad hasta del 4% entre sistemas de vagones livianos y pesados. Además, los vagones livianos con manejo adecuado de la cosecha, llegan a ser favorables por menor consumo de combustible y emisiones.This study was carried out to determine the impact of traffic on soil compaction, crop and energy consumption during the sugarcane harvest in the Cauca river valley (Colombia. Four experiments with four replicates were harvested with different systems. Plots were mechanically harvested with self tipping, HD8000, HD12000 and HD20000 trailers and evaluated by traffic intensity (IT, direct stool traffic, penetration resistance and fuel consumption. Heavy trailers caused a greater effect due to a greater IT and direct stool traffic. IT varied between 241 and 317 Mg km ha-1. Stool traffic at the end of field varied from 8 to 18 m per furrow, meanwhile stool traffic along the furrow varied from 5 to 24

  13. Trækonstruktioner

    DEFF Research Database (Denmark)

    Larsen, H.J.; Riberholt, H.

    Denne anvisning behandler forbindelser i trækonstruktioner i henhold til de normer for bærende konstruktioner, der er udkommet i 1982. Den knytter sig til SBI-anvisning 135: Trækonstruktioner, Beregning. Anvisningen er tænkt anvendt både af projekterende og som lærebog på ingeniørskolerne....

  14. Tämän päivän johtajuus : Leadership vai Management

    OpenAIRE

    Satokangas, Kim

    2014-01-01

    Opinnäytetyön aiheena on Tämän päivän johtajuus, Leadership vai Management. Työn tavoitteena on tutkia mitä, tämänpäivän johtajuus on ja kuinka management ja leadership ilmenevät tämän päivän johtamistyön arjessa. Tarkoituksena on selvittää milloin ja missä asioissa tarvitaan leadership-tyylistä johtamista ja milloin ja missä asioissa tarvitaan management-tyylin johtajuutta. Opinnäytetyö on toteutettu laadullisena opinnäytetyönä. Se lähtee liikkeelle käsittelemällä johtamista yleisesti j...

  15. Nikolā Abanjāno pozitīvais eksistenciālisms

    OpenAIRE

    Sprudzāne, Kate

    2010-01-01

    Pozitīvais eksistenciālisms ir nozīmīgs 20. gadsimta filosofijas pieteikums, kas Latvijā ir aizmirsts pilnībā. Eksistenciālisma, tāpat kā eksistences filosofijas, nozīme nav pārvērtējama: tas ir ietekmējis 20. gadsimta norises un transformējis cilvēku pašizpratnes un pasaules apjēguma fornas. Pozitīvā eksistenciālisma pamatlicējs, itāliešu pirmais eksistenciālists, Nikolā Abanjāno ir izceļams ar to, ka paver pozitīvu (iespējamu) eksistences attiecību ar esamību izveidi. Darba mērķis ir atsegt...

  16. Yrittäjä opettaja vai opettava yrittäjä?

    OpenAIRE

    Silvennoinen-Lampinen, Minna

    2006-01-01

    Yrittäjyys on tärkeä yhteiskunnallinen ilmiö. Työni tavoitteena on ollut perehtyä Euroopan Unionin ja Suomen tavoitteisiin yrittäjyyden lisäämiseksi koulutuksen avulla. Käsittelen työssäni yrittäjyyden edistämiseksi tehtyjä toimia, yrittäjyyskasvatuksen lähtökohtaa ja - määritelmää sekä yrittäjyyden oppimisprosessia. Pohdin työni aikana samalla suhdettani omaan opettajuuteeni. Olenko yrittäjämäinen opettaja vai opettava yrittäjä. Yrittäjyyden kannalta ammatillisessa koulutuksessa keskeisiä te...

  17. A acupuntura vai além da agulha: trajetórias de formação e atuação de acupunturistas

    Directory of Open Access Journals (Sweden)

    Marcelo Felipe Nunes

    Full Text Available Resumo O objetivo deste estudo foi analisar as trajetórias de formação e a atuação profissional de acupunturistas. Trata-se de estudo de natureza qualitativa, baseado no referencial metodológico das narrativas de histórias de vida. Participaram oito profissionais de saúde - cinco homens e três mulheres -, graduados em enfermagem, fisioterapia e medicina, que utilizavam a acupuntura a partir da perspectiva da medicina chinesa (MC e que responderam a entrevistas semiestruturadas. A análise das narrativas resultou em três categorias centrais: busca por novas racionalidades em saúde; adentrando no universo da acupuntura; a acupuntura vai além da agulha. Observou-se que a busca pela acupuntura foi motivada pela insatisfação com a formação inicial no paradigma biomédico. A trajetória de formação na acupuntura foi gradativa e envolveu profunda inserção na racionalidade da MC, devido à complexidade de sua proposta terapêutica, o que os levou a entender que a inserção da agulha representa a confluência de diversos aspectos da racionalidade, não podendo ser reduzida a mera aplicação técnica. Nesse sentido, aponta-se a necessidade de discutir a formação desses profissionais e sua atuação no Sistema Único de Saúde, bem como a aplicabilidade da perspectiva biomédica de pesquisa nos estudos sobre acupuntura.

  18. Changes of Benthic Macroinvertebrates in Thi Vai River and Cai Mep Estuaries Under Polluted Conditions with Industrial Wastewater

    Directory of Open Access Journals (Sweden)

    Huong Nguyen Thi Thanh

    2017-06-01

    Full Text Available The pollution on the Thi Vai River has been spreading out rapidly over the two lasted decades caused by the wastewater from the industrial parks in the left bank of Thi Vai River and Cai Mep Estuaries. The evaluation of the benthic macroinvertebrate changes was very necessary to identify the consequences of the industrial wastewater on water quality and aquatic ecosystem of Thi Vai River and Cai Mep Estuaries. In this study, the variables of benthic macroinvertebrates and water quality were investigated in Thi Vai River and Cai Mep Estuaries, Southern Vietnam. The monitoring data of benthic macroinvertebrates and water quality parameters covered the period from 1989 to 2015 at 6 sampling sites in Thi Vai River and Cai Mep Estuaries. The basic water quality parameters were also tested including pH, dissolved oxygen (DO, total nitrogen, and total phosphorus. The biodiversity indices of benthic macroinvertebrates were applied for water quality assessment. The results showed that pH ranged from 6.4 – 7.6 during the monitoring. The DO concentrations were in between 0.20 - 6.70 mg/L. The concentrations of total nitrogen and total phosphorous ranged from 0.03 - 5.70 mg/L 0.024 - 1.380 mg/L respectively. Macroinvertebrate community in the study area consisted of 36 species of polychaeta, gastropoda, bivalvia, and crustacea, of which, species of polychaeta were dominant in species number. The benthic macroinvertebartes density ranged from 0 - 2.746 individuals/m−1 with the main dominant species of Neanthes caudata, Prionospio malmgreni, Paraprionospio pinnata, Trichochaeta carica, Maldane sarsi, Capitella capitata, Terebellides stroemi, Euditylia polymorpha, Grandidierella lignorum, Apseudes vietnamensis. The biodiversity index values during the monitoring characterized for aquatic environmental conditions of mesotrophic to polytrophic. Besides, species richness positively correlated with DO, total nitrogen, and total phosphorus. The results

  19. TR YPANOSOMA VIVAX

    African Journals Online (AJOL)

    TR YPANOSOMA VIVAX. IJAGBONE, I. F., ESURUOSO, G. O. AND AGBEDE, S. A. Department of Veterinary Public Health and Preventive Medicine. University of Ibadan, Ibadan, Nigeria. SUMMARY 5. A group of West African Dwarf goats was experimentally challenged with an isolate of Trypanosoma vivax. 'Y58' stock.

  20. Visceral adiposity index (VAI is predictive of an altered adipokine profile in patients with type 2 diabetes.

    Directory of Open Access Journals (Sweden)

    Marco C Amato

    Full Text Available AIMS: Although there is still no clear definition of "adipose tissue dysfunction" or ATD, the identification of a clinical marker of altered fat distribution and function may provide the needed tools for early identification of a condition of cardiometabolic risk. Our aim was to evaluate the correlations among various anthropometric indices [BMI, Waist Circumference (WC, Hip Circumference (HC, Waist/Hip ratio (WHR, Body Adiposity Index (BAI and Visceral adiposity Index (VAI] and several adipocytokines [Visfatin, Resistin, Leptin, Soluble leptin receptors (sOB-R, Adiponectin, Ghrelin, Adipsin, PAI-1, vascular endothelial growth factor (VEGF, Hepatocyte growth factor (HGF TNF-α, hs-CRP, IL-6, IL-18] in patients with type 2 diabetes (DM2. MATERIALS AND METHODS: Ninety-one DM2 patients (age: 65.25 ± 6.38 years; 42 men and 49 women in stable treatment for the last six months with metformin in monotherapy (1.5-2 g/day were cross-sectionally studied. Clinical, anthropometric, and metabolic parameters were evaluated. Serum adipocytokine levels were assayed with Luminex based kits. RESULTS: At the Pearson's correlation, among all the indices investigated, VAI showed a significant correlation with almost all adipocytokines analyzed [Visfatin, Resistin and hsCRP (all p<0.001; Adiponectin, sOb-R, IL-6, IL-18, HGF (all p<0.010; Ghrelin and VEGF (both p<0.05]. Through a two-step cluster analysis, 55 patients were identified with the most altered adipocytokine profile (patients with ATD. At a ROC analysis, VAI showed the highest C-statistic [0.767 (95% CI 0.66-0.84] of all the indices. CONCLUSIONS: Our study suggests that the VAI, among the most common indexes of adiposity assessment, shows the best correlation with the best known adipocytokines and cardiometabolic risk serum markers. Although to date we are still far from clearly identifying an ATD, the VAI would be an easy tool for clearly mirroring a condition of cardiometabolic risk, in the absence of an

  1. Francisco Carlos Teixeira da Silva, Karl Shurster Sousa Leão, Igor Lapsky (organizadores), O Cinema Vai à Guerra

    OpenAIRE

    Santiago Júnior, Francisco das Chagas F.

    2016-01-01

    O Cinema vai à Guerra, libro curato da Francisco Carlos T. da Silva, Karl Schurster Leão e Igor Lapsky si inserisce nel solco della tradizione storiografica brasiliana attenta alla relazione fra storia e cinema. Il tema della guerra nel cinema non è nuovo né nello scenario internazionale, né in Brasile, non essendo, peraltro, monopolio della ricerca storica. La maggior parte delle monografie precedenti, infatti sono state scritte – in ambito brasiliano – da studiosi o critici cinematografici....

  2. Peletização do lodo de esgoto e adição de CaCO3 na produção de matéria seca e absorção de Zn, Cu e Ni pelo milho em três latossolos

    Directory of Open Access Journals (Sweden)

    R. S. Berton

    1997-12-01

    Full Text Available Em experimento de casa de vegetação, realizado em Campinas (SP, no período de janeiro a maio de 1980, estudaram-se os efeitos da peletização do lodo de esgoto, nas doses 0, 1 e 5% (v/v do material seco, na produção de matéria seca e na absorção de Zn, Cu e Ni pela parte aérea do milho (Zea mays L., em latossolo roxo (LR, latossolo vermelho-escuro (LE e latossolo vermelho-amarelo (LV, que receberam ou não adição de CaCO3 suficiente para elevação do pH em água para 6,0. O experimento foi realizado em vasos com dois litros de capacidade, delineados em blocos ao acaso, com três repetições, e os tratamentos arranjados num esquema fatorial. Após 200 dias de incubação dos solos com o lodo e CaCO3, cultivaram-se quatro plantas de milho em cada vaso. A parte aérea foi quantificada 56 dias após a germinação, sendo cortada, seca, pesada e analisada para os elementos Zn, Cu e Ni. Amostras de terra de cada tratamento foram retiradas e tiveram os metais extraídos pelo DTPA. A peletização do lodo de esgoto resultou em diminuição significativa na produção de matéria seca pela parte aérea do milho, nos três solos estudados, em comparação com o lodo não peletizado. A adição de CaCO3 proporcionou aumento significativo na produção de matéria seca do milho apenas nos dois solos mais ácidos (LE e LV e diminuiu o acúmulo de Zn na parte aérea desse vegetal cultivado no LE, sem distinção quanto ao tipo de lodo aplicado, não se observando diferenças significativas na absorção de Ni, em todos os solos analisados. A incorporação do lodo peletizado , que continha 19% menos Zn e Ni que o não peletizado, resultou em menor absorção de Zn, Cu e Ni nos três solos, exceto para o Ni no LR. Para todos os solos investigados, o método do DTPA mostrou-se mais adequado em prognosticar as quantidades disponíveis de Zn e de Cu para o milho, independentemente do tipo de lodo de esgoto aplicado e da elevação ou não do pH do

  3. AS IMPLICAÇÕES DA CULTURA ORGANIZACIONAL NO PROCESSO DE INOVAÇÃO NAS ESCOLAS DE SAMBA DA CIDADE DE SÃO PAULO: O CASO DA ESCOLA DE SAMBA VAI VAI

    Directory of Open Access Journals (Sweden)

    Márcio Cardoso Santos

    2011-12-01

    Full Text Available Já se tornou notória a mobilização de milhares de pessoas para prestigiar o carnaval paulistano. Como o evento é atrativo turístico da cidade, é considerado motivo para investimentos do governo em obras públicas. Diante da relevância do evento carnavalesco, da estrutura complexa das agremiações e o alto nível de competitividade nos desfiles, este trabalho tem como propósito explorar o carnaval como fonte de inovação e organização. Acredita-se que o evento carnavalesco deve ser analisado não só como espetáculo, mas como fonte de teorias para a área dos estudos organizacionais. Como a investigação compreende um objeto pouco comum, as Escolas de Samba, o procedimento metodológico que melhor se enquadrou foi uma pesquisa exploratória de cunho empírico, por meio de estudo de caso e levantamento bibliográfico. A liderança é responsável por um ambiente de incentivo a interação e o aprendizado contínuo, enfatizando as competências e habilidades dos integrantes, como meio de promover ações inovadoras. Compreende-se que a inovação exige um processo metodológico para ser adotada e desenvolvida nas organizações, mas já foi possível notar que a Escola de Samba Vai Vai, dispõe de ambiente e histórico propício para ações de inovação, o que provavelmente influencia o seu bom desempenho nos desfiles.

  4. Exercício e restrição alimentar aumentam o RNAm de proteínas do trânsito de Ca2+ miocárdico em ratos

    Directory of Open Access Journals (Sweden)

    Mário Mateus Sugizaki

    2011-07-01

    Full Text Available FUNDAMENTO: Treinamento físico (TF aumenta a sensibilidade dos hormônios tireoidianos (HT e a expressão gênica de estruturas moleculares envolvidas no movimento intracelular de cálcio do miocárdio, enquanto a restrição alimentar (RIA promove efeitos contrários ao TF. OBJETIVO: Avaliar os efeitos da associação TF e RIA sobre os níveis plasmáticos dos HT e a produção de mRNA dos receptores HT e estruturas moleculares do movimento de cálcio do miocárdio de ratos. MÉTODOS: Utilizaram-se ratos Wistar Kyoto divididos em: controle (C, n = 7, RIA (R50, n = 7, exercício físico (EX, n = 7 e exercício físico + RIA (EX50, n = 7. A RIA foi de 50% e o TF foi natação (1 hora/dia, cinco sessões/semana, 12 semanas consecutivas. Avaliaram-se as concentrações séricas de triiodotironina (T3, tiroxina (T4 e hormônio tireotrófico (TSH. O mRNA da bomba de cálcio do retículo sarcoplasmático (SERCA2a, fosfolamban (PLB, trocador Na+/Ca+2 (NCX, canal lento de cálcio (canal-L, rianodina (RYR, calsequestrina (CQS e receptor de HT (TRα1 e TRβ1 do miocárdio foram avaliados por reação em cadeia da polimerase (PCR em tempo real. RESULTADOS: RIA reduziu o T4, TSH e mRNA do TRα1 e aumentou a expressão da PLB, NCX e canal-L. TF aumentou a expressão do TRβ1, canal-L e NCX. A associação TF e RIA reduziu T4 e TSH e aumentou o mRNA do TRβ1, SERCA2a, NCX, PLB e correlação do TRβ1 com a CQS e NCX. CONCLUSÃO: Associação TF e RIA aumentou o mRNA das estruturas moleculares cálcio transiente, porém o eixo HT-receptor não parece participar da transcrição gênica dessas estruturas.

  5. Exercício e restrição alimentar aumentam o RNAm de proteínas do trânsito de Ca2+ miocárdico em ratos Ejercicio y restricción alimenticia aumentan el rnam de proteínas del tránsito de Ca2+ miocárdico en ratones Upregulation of mRNA myocardium calcium handling in rats submitted to exercise and food restriction

    Directory of Open Access Journals (Sweden)

    Mário Mateus Sugizaki

    2011-07-01

    Full Text Available FUNDAMENTO: Treinamento físico (TF aumenta a sensibilidade dos hormônios tireoidianos (HT e a expressão gênica de estruturas moleculares envolvidas no movimento intracelular de cálcio do miocárdio, enquanto a restrição alimentar (RIA promove efeitos contrários ao TF. OBJETIVO: Avaliar os efeitos da associação TF e RIA sobre os níveis plasmáticos dos HT e a produção de mRNA dos receptores HT e estruturas moleculares do movimento de cálcio do miocárdio de ratos. MÉTODOS: Utilizaram-se ratos Wistar Kyoto divididos em: controle (C, n = 7, RIA (R50, n = 7, exercício físico (EX, n = 7 e exercício físico + RIA (EX50, n = 7. A RIA foi de 50% e o TF foi natação (1 hora/dia, cinco sessões/semana, 12 semanas consecutivas. Avaliaram-se as concentrações séricas de triiodotironina (T3, tiroxina (T4 e hormônio tireotrófico (TSH. O mRNA da bomba de cálcio do retículo sarcoplasmático (SERCA2a, fosfolamban (PLB, trocador Na+/Ca+2 (NCX, canal lento de cálcio (canal-L, rianodina (RYR, calsequestrina (CQS e receptor de HT (TRα1 e TRβ1 do miocárdio foram avaliados por reação em cadeia da polimerase (PCR em tempo real. RESULTADOS: RIA reduziu o T4, TSH e mRNA do TRα1 e aumentou a expressão da PLB, NCX e canal-L. TF aumentou a expressão do TRβ1, canal-L e NCX. A associação TF e RIA reduziu T4 e TSH e aumentou o mRNA do TRβ1, SERCA2a, NCX, PLB e correlação do TRβ1 com a CQS e NCX. CONCLUSÃO: Associação TF e RIA aumentou o mRNA das estruturas moleculares cálcio transiente, porém o eixo HT-receptor não parece participar da transcrição gênica dessas estruturas.FUNDAMENTO: Entrenamiento físico (EF aumenta la sensibilidad de las hormonas tiroideas (HT y la expresión génica de estructuras moleculares envueltas en el movimiento intracelular de calcio del miocardio, mientras que la restricción alimenticia (RA promueve efectos contrarios al EF. OBJETIVO: Evaluar los efectos de la asociación EF y RA sobre los niveles

  6. Neuralgia trójdzielna

    Directory of Open Access Journals (Sweden)

    Jan Kochanowski

    2016-06-01

    Full Text Available Neuralgia trójdzielna należy do najcięższych bólów w obrębie twarzy. Choć została opisana przez wielu badaczy, dzisiaj nazywa się ją chorobą Fothergilla, co upamiętnia przedstawienie neuralgii trójdzielnej przez tego uczonego w 1773 roku. Zachorowalność jest oceniana na 2–5/100 tys. osób, częściej chorują kobiety. Nerw trójdzielny to nerw czuciowo-ruchowy, który wspólnie z nerwami językowo-gardłowym i błędnym, nerwami podpotylicznym, potylicznymi mniejszymi i większymi oraz nerwem usznym wielkim unerwia skórę głowy. Włókna przewodzące bodźce bólowe przez zwój nerwu trójdzielnego kończą się w jądrze rdzeniowym, skąd przez jądra wzgórza docierają do kory czuciowej, układu limbicznego i wyspy. Przyczyn bólu w neuralgii upatruje się w uszkodzeniu włókien czuciowych grubych Aβ i powstałych ogniskach ektopowego pobudzenia – przenoszonego na uszkodzone strukturalnie włókna cienkie Aδ i C w obrębie połączeń zwanych efapsami. Neuralgię trójdzielną dzieli się na klasyczną i objawową. Obie postacie charakteryzują się napadami bólu o typie rażenia prądem elektrycznym, trwającymi od kilku sekund do dwóch minut. Ból najczęściej dotyczy obszaru unerwionego przez drugą i trzecią gałąź nerwu trójdzielnego, czyli nerw szczękowy i żuchwowy. Cechą charakterystyczną są strefy spustowe, których drażnienie przez bodźce niebólowe wywołuje napad neuralgii. Leczenie neuralgii trójdzielnej obejmuje postępowanie zachowawcze i chirurgiczne. W leczeniu zachowawczym złotym standardem jest stosowanie karbamazepiny lub okskarbazepiny. W przypadku niepowodzenia można włączyć baklofen, lamotryginę albo inny lek używany w leczeniu padaczki. Spośród metod chirurgicznych najczęściej stosuje się: odbarczenie konfliktu naczyniowo-nerwowego, ucisk zwoju troistego balonem, przezskórną blokadę zwoju Gassera glicerolem, przecięcie włókien zazwojowych nerwu tr

  7. [On a comparison between the theory of movement and the theory of Karma in Vaiśesika system in ancient India].

    Science.gov (United States)

    Ohami, I

    1993-01-01

    The action of a human body is composed of a series of momentary movements caused by a volitional effort on the human in the Vaiśesika system. According to the theory of Karma in the Vaiśesika system, when an action is done by a person, it leaves an ethical potency, so called karmā'saya on one's mind. When the potency, karmā'saya is accompanied with the other potency, affliction so called kle'sa in one's mind, it grows up as the seed of a plant and bears the fruit of it. Any person who has the fruit of karmā'saya in one's mind, is made to experience joy or sorrow in the future in accordance with the judgement of the Creator about the fruit of karmā'saya. The affliction is the emotional potency caused by ignorance, e.g. avarice. On the other hand, the first movement of matter is produced by the movement of a mover through the special contact, so called nodana. The first movement produces the vega (the power of motion) in the moved matter. Through nodana, the movement and the vega are correlatively increased. The nodana is impulsive contact, acting between a mover and the moved matter. As I have shown in my paper, "On the theory of movement in Vaiśesika system iin ancient India (II)", the above process of movements of matter through nodana is very similar to the mental process of remembrance of a person. The growing process of the ethical potency, karmā'saya in a person's mind is, however, different to the above process of movements of matter. The intensity of the vega is "temporarily" increased through the contact, nodana, but the karmā'saya is "perpetually" grown by the potency, affliction until the karmā'saya bears the fruit of it.

  8. Luonnonkosmetiikkaa vai ei?

    OpenAIRE

    Kiuttu, Katja

    2010-01-01

    Tämän opinnäytetyön tavoitteena oli selvittää, mitä luonnonkosmetiikalla tarkoitetaan. Työssä pohdittiin, miksi kosmetiikkaa mainostetaan luonnolla. Työssä tutustuttiin luonnonkosmetiikan sertifikaatteihin sekä niiden eroihin. Lisäksi paneuduttiin luonnonkosmetiikan sallittuihin ja kiellettyihin raaka-aineisiin ja pohdittiin kiellettyjen aineiden etuja. Opinnäytetyössä ei haettu vastausta kysymykseen, onko luonnonkosmetiikka tavallista kosmetiikkaa parempaa. Opinnäytetyö toteutettiin asia...

  9. MARANHÃO FILHO, Eduardo Meinberg de Albuquerque. A grande onda vai te pegar: Marketing, espetáculo e ciberespaço na Bola de Neve Church.

    Directory of Open Access Journals (Sweden)

    Manuela Lowenthal Ferreira

    2014-03-01

    Full Text Available MARANHÃO FILHO, Eduardo Meinberg de Albuquerque. A grande onda vai te pegar: Marketing, espetáculo e ciberespaço na Bola de Neve Church. São Paulo: Fonte Editorial, 2013.A resenha diz respeito ao livro "A grande onda vai te pegar, espetáculo e ciberespaço na Bola de Neve Church's", na qual trata das transformações no campo religioso hoje, a partir do estudo de caso com a igreja Neopentecostal "Bola de Neve Church1s".

  10. New targetry possibilities from the TR-24

    OpenAIRE

    Zyuzin, A.; Sader, J.; Jenei, E.; Tremblay, S.; Thibault, P.; Guerin, B

    2015-01-01

    Introduction The TR-24 is relatively new to the cyclotron market and its advantages over lower energy PET cyclotrons have not yet been fully realized. A new high current [18F] fluoride production target that takes advantage of the higher energy and current afforded by the TR-24 has been developed. Material and Methods The TR-24 cyclotron presents challenges of producing conventional PET isotopes even with its variable energy capability (18–25 MeV). Simultaneous irradiation of two ta...

  11. Friluftsinstallationer i træer

    DEFF Research Database (Denmark)

    Skov, Simon; Thomsen, Iben Margrete

    2015-01-01

    Abebaner, treetop walking, high rope adventure, slackline, træhuse og parkour-baner er alle installationer, som bruger træerne som støtte, enten midlertidigt eller permanent. Den nye sport, turistattraktionen eller team building-redskabet sætter i alle tilfælde træerne på prøve. Der følger positiv...

  12. Fram träder arbetaren

    DEFF Research Database (Denmark)

    Christensen, Lars K.

    2011-01-01

    Anmeldelse af Bosdotter og Isacsson: Fram träder arbetaren: Arbetarkonst och industrisamhällets bilder i Norden. Stockholm 2009.......Anmeldelse af Bosdotter og Isacsson: Fram träder arbetaren: Arbetarkonst och industrisamhällets bilder i Norden. Stockholm 2009....

  13. The Novel Antitubulin Agent TR-764 Strongly Reduces Tumor Vasculature and Inhibits HIF-1α Activation.

    Science.gov (United States)

    Porcù, Elena; Persano, Luca; Ronca, Roberto; Mitola, Stefania; Bortolozzi, Roberta; Romagnoli, Romeo; Oliva, Paola; Basso, Giuseppe; Viola, Giampietro

    2016-06-13

    Tubulin binding agents (TBAs) are commonly used in cancer therapy as antimitotics. It has been described that TBAs, like combretastatin A-4 (CA-4), present also antivascular activity and among its derivatives we identified TR-764 as a new inhibitor of tubulin polymerization, based on the 2-(alkoxycarbonyl)-3-(3',4',5'-trimethoxyanilino)benzo[b]thiophene molecular skeleton. The antiangiogenic activity of TR-764 (1-10 nM) was tested in vitro on human umbilical endothelial cells (HUVECs), and in vivo, on the chick embryo chorioallantoic membrane (CAM) and two murine tumor models. TR-764 binding to tubulin triggers cytoskeleton rearrangement without affecting cell cycle and viability. It leads to capillary tube disruption, increased cell permeability, and cell motility reduction. Moreover it disrupts adherens junctions and focal adhesions, through mechanisms involving VE-cadherin/β-catenin and FAK/Src. Importantly, TR-764 is active in hypoxic conditions significantly reducing HIF-1α. In vivo TR-764 (1-100 pmol/egg) remarkably blocks the bFGF proangiogenic activity on CAM and shows a stronger reduction of tumor mass and microvascular density both in murine syngeneic and xenograft tumor models, compared to the lead compound CA-4P. Altogether, our results indicate that TR-764 is a novel TBA with strong potential as both antivascular and antitumor molecule that could improve the common anticancer therapies, by overcoming hypoxia-induced resistance mechanisms.

  14. Landbrugets trædemølle

    DEFF Research Database (Denmark)

    Hansen, Henning Otte

    2016-01-01

    Teorien om landbrugets trædemølle siger, at teknologi medfører stigende produktivitet, stigende udbud og dermed faldende priser. Dermed øges behovet for ny teknologi. Det vedvarende teknologipres gavner de innovative landmænd, mens de mere afventende landmænd kun oplever de negative virkninger i...... form af prisfald. I denne artikel beskrives nærmere de enkelte elementer i trædemøllen. Samtidig vurderes trædemøllens betydning og mulige påvirkning. Det konkluderes, at trædemøllen, dens forudsætninger og afledte virkninger stadig er fuldt gældende. Det er ikke muligt for et enkelt land eller region...... af bremse trædemøllen på lang sigt. På lokalt plan kan man løse nogle sociale og økonomiske problemer skabt af trædemøllen gennem nemmere afvandring....

  15. A acupuntura vai além da agulha: trajetórias de formação e atuação de acupunturistas

    National Research Council Canada - National Science Library

    Marcelo Felipe Nunes; José Roque Junges; Tonantzin Ribeiro Gonçalves; Monique Adriane Motta

    .... Participaram oito profissionais de saúde - cinco homens e três mulheres -, graduados em enfermagem, fisioterapia e medicina, que utilizavam a acupuntura a partir da perspectiva da medicina chinesa (MC...

  16. "Raamatusalongis" briti kaasaegne trükikunst

    Index Scriptorium Estoniae

    2002-01-01

    7.-17. XI Tallinna Linnagaleriis Briti Nõukogu näitus "Raamatusalong", mis annab ülevaate briti kaasaegsest trükikunstist. Kuraator Emily King on valinud näitusele 30 ajakirja ja 300 raamatut (kunst, disain jm.). Näituse graafilise kujunduse tegi ¿bäke, mööbli kujundas Martino Gamper

  17. Naturlig ventilation og træk

    DEFF Research Database (Denmark)

    Nielsen, Peter Vilhelm

    2002-01-01

    Nye ventilationsprincipper som naturlig ventilation er med til at sætte fokus på de strømningselementer, der skal anvendes til dimensionering af luftfordelingen i et rum. Artiklen anviser, hvordan træk fra vinduer og tagåbninger i rum med naturlig ventilation kan beregnes ved hjælp af...

  18. Blog.tr.ee ei anna alla / Raigo Neudorf

    Index Scriptorium Estoniae

    Neudorf, Raigo

    2007-01-01

    Veebikeskkonna blog.tr.ee arengust ja majandamisest räägivad keskkonna looja Andris Reinmann ja firma OÜ Tr.ee osaniku Mobi Solutions'i esindaja Rain Rannu. Vt. samas: Mis on blog.tr.ee; Kaljundi: blog.tr.ee on hobiprojekt. Kommenteerib nagi.ee juhataja Jüri Kaljundi. Lisatud joonis: Blog.tr.ee unikaalsete külastajate arv nädalas

  19. VIDA-træningsprogram til ledelsesfacilitering af læreprocesser

    DEFF Research Database (Denmark)

    Ravn, Ib; Tange, Nina

    2013-01-01

    Som led i VIDA-projektet blev de ca. 80 deltagende ledere af daginstitutioner tilbudt træningsforløb i facilitering. Facilitering er en tilgang, der sætter ledere i stand til at guide og støtte medarbejderinddragelsen på en sådan måde, at alle føler sig hørt, og der bliver skabt resultater på...

  20. Neuroblastomicose: registro de três casos

    Directory of Open Access Journals (Sweden)

    Ehrenfried O. Wittig

    1968-03-01

    Full Text Available São relatados três casos de neuroblastomicose de sintomatologia variada (urna forma tumoral cerebelar, urna forma abscedante troncular e cerebral e urna forma granulomatosa difusa. No caso da forma tumoral cerebelar havia associação com cisticercose cerebral. Alterações medulares não foram evidenciadas no único caso em que foi possível examinar êste setor do sistema nervoso central (caso 2.

  1. Vejledningens terminologiske trængsler

    DEFF Research Database (Denmark)

    Lystbæk, Christian Tang

    2014-01-01

    Vejledning har vundet stor udbredelse. Men mange oplever, at vejledning bruges i flæng og til tider misbruges, eksempelvis som led i en fagpolitisk kamp eller en økonomisk spareøvelse, eksempelvis i forbindelse med reformer på uddannelses- og arbejdsmarkedsområdet. Artiklen peger på, at oplevelsen...... af vilkår brug og til tider decideret misbrug af begreber om vejledning skyldes, at der er en voldsom trængsel af begreber om vejledning, som forskellige interessenter i vejledningsfeltet konkurrerer om at give mening til. Vejledning har ikke én fast betydning eller én grundlæggende idé, men sætter...... forskellige pædagogiske og politiske idéer og rationaler på en fælles formel. Som sådan skyldes den voldsomme trængsel af begreber i vejledningsfeltet, at vejledning er gennemsyret af, hvad man kan kalde ”terminologiske trængsler”, dvs. af omstridte begreber, som forskellige pædagogiske og politiske...

  2. TR-644 a novel potent tubulin binding agent induces impairment of endothelial cells function and inhibits angiogenesis.

    Science.gov (United States)

    Porcù, Elena; Viola, Giampietro; Bortolozzi, Roberta; Persano, Luca; Mitola, Stefania; Ronca, Roberto; Presta, Marco; Romagnoli, Romeo; Baraldi, Pier Giovanni; Basso, Giuseppe

    2013-07-01

    TR-644 is a novel combretastatin A-4 (CA-4) analogue endowed with potent microtubule depolymerizing activity superior to that of the lead compound and it also has high affinity to colchicines binding site of tubulin. We tested TR-644 anti-angiogenic effects in human umbilical endothelial cells (HUVEC). It showed no significant effects on the growth of HUVEC cells at concentrations below 1,000 nM, but at much lower concentrations (10-100 nM) it induced inhibition of capillary tube formation, inhibition of endothelial cell migration and affected endothelial cell morphology as demonstrated by the disruption of the microtubule network. TR-644 also increased permeability of HUVEC cells in a time dependent manner. The molecular mechanism for the anti-vascular activity of TR-644 was investigated in detail. TR-644 caused G2/M arrest in endothelial cells and this effect correlated with downregulation of the expression of Cdc25C and Cdc2(Tyr15). Moreover TR-644 inhibited VEGF-induced phosphorylation of VE-cadherin but did not prevent the VEGF-induced phosphorylation of FAK. In chick chorioallantoic membrane in vivo assay, TR-644 (0.1-1.0 pmol/egg) efficiently counteracted the strong angiogenic response induced by FGF. Also CA-4, used as reference compound, caused an antagonistic effect, but in contrast, it induced per se, a remarkable angiogenic response probably due to an inflammatory reaction in the site of treatment. In a mice allogenic tumor model, immunohistochemical staining of tumors with anti-CD31 antibody showed that TR-644 significantly reduced the number of vessel, after 24 h from the administration of a single dose (30 mg/Kg).

  3. Aonde vai o Trabalho Humano?

    Directory of Open Access Journals (Sweden)

    Henrique Rattner

    2010-07-01

    Full Text Available

    Foi este o título de um livro baseado em pesquisa na indústria francesa, do sociólogo Georges Friedmann, publicado no início da década dos cinqüenta do século passado. Logo depois, Friedmann publicou outra pesquisa em livro intitulado “O trabalho em migalhas” (Le travail em mièttes, no qual analisa criticamente o fracionamento das operações de trabalho e o começo da automação, ambos ilustrados no inesquecível filme de Charles Chaplin, “Tempos Modernos”.

  4. Para onde vai a Geografia?

    Directory of Open Access Journals (Sweden)

    Angélica Karina Dillenburg Horii

    2015-12-01

    Full Text Available O presente artigo aborda a evolução da Geografia enquanto pensamento científico, analisando seu objeto de estudo e suas categorias de análise durante todo o percurso da criação desse saber. Observa primeiramente a formação da Geografia Clássica, que teve seus princípios já instituídos na Antiguidade com Ptolomeu e Estrabão, desdobrando-se no período Iluminista com Ritter e Humboldt que fornecerão uma nova temporalidade a essa ciência. Atinge seu ápice na Modernidade Industrial com a racionalidade de Kant, onde Ratzel e La Blache utilizarão o determinismo e o possibilismo para constituir um discurso científico para a Geografia. Finaliza apontando a nova compreensão do mundo, conhecida como pós-modernismo por David Harvey (1992 ou por Milton Santos (1996 como Globalização, que junto a outros geógrafos e pesquisadores, indicam caminhos de análise dessa ciência na compreensão do seu objeto de estudo. Um diálogo entre os autores na construção do pensamento geográfico.

  5. 3rd MeTrApp Conference

    CERN Document Server

    Lovasz, Erwin-Christian; Hüsing, Mathias

    2015-01-01

    This volume deals with topics such as mechanism and machine design, biomechanics and medical engineering, gears, mechanical transmissions, mechatronics, computational and experimental methods, dynamics of mechanisms and machines, micromechanisms and microactuators, and history of mechanisms and transmissions. Following MeTrApp 2011 and 2013, held under the auspices of the IFToMM, these proceedings of the 3rd Conference on Mechanisms, Transmissions and Applications offer a platform for original research presentations for researchers, scientists, industry experts and students in the fields of mechanisms and transmissions with special emphasis on industrial applications in order to stimulate the exchange of new and innovative ideas.

  6. Atresia de tráquea

    Directory of Open Access Journals (Sweden)

    Rafael Amador Morán

    2010-12-01

    Full Text Available La atresia de tráquea es una afección infrecuente en el recién nacido y la mayoría de las veces se asocia a otras malformaciones complejas sobre las cuales se han registrado en la literatura médica no más de 100 casos. Se documenta el caso de un recién nacido varón con atresia traqueal y fístula traqueoesofágica, anomalías cardíacas y gastrointestinales, que tuvo una supervivencia de 81 h.

  7. Trükikoda Edes (Holland) / Triin Ojari

    Index Scriptorium Estoniae

    Ojari, Triin, 1974-

    1998-01-01

    Veenmani trükikoja uus peakontor. Arhitekt Jan Willem Neutelings. Siseõuega kastmaja mahutab administratsiooni ja trükikoja ruumid. Hoone fassaad on kaetud trükitähtedega, mis moodustavad luuletuse. Valge fassaad kujundatakse igal aastal ümber.

  8. Trükitööstuste TOP 50

    Index Scriptorium Estoniae

    2002-01-01

    Trükitööstuste TOP 50. Käibe TOP 30. Käibe kasum TOP 30. Kasvu TOP 30. Kasumi kasvu TOP 30. Rentaabluse TOP. Varade tootlikkuse TOP. Trükitööstusettevõtete üldandmed. Trükitööstusettevõtete finantsandmed

  9. In vivo recognition of cyclopentadienyltricarbonylrhenium (CpTR) derivatives

    Energy Technology Data Exchange (ETDEWEB)

    Uehara, Tomoya; Koike, Miho; Nakata, Hideo; Miyamoto, Shigehiko; Motoishi, Shoji; Hashimoto, Kazuyuki; Oku, Naoto; Nakayama, Morio; Arano, Yasushi E-mail: arano@p.chiba-u.ac.jp

    2003-04-01

    In vivo metabolism of [{sup 188}Re]tricarbonyl(carboxycyclopentadienyl)rhenium ([{sup 188}Re]CpTR-COOH) and its glycine conjugate ([{sup 188}Re]CpTR-Gly) was investigated to estimate the applicability of cyclopentadienyltricarbonylrhenium (CpTR) compounds to {sup 186/188}Re-labeling reagents for polypeptides and peptides. Both [{sup 188}Re]CpTR derivatives were stable after incubation in a buffered-solution and in murine plasma at 37 deg. C for 6 h. Plasma protein binding was hardly observed with the two derivatives. However, different biodistribution and metabolic fates were observed with the two CpTR derivatives. While more lipophilic [{sup 188}Re]CpTR-COOH was excreted by both hepatobiliary and urinary excretion, the majority of less lipophilic [{sup 188}Re]CpTR-Gly was excreted by urinary excretion. In addition, while [{sup 188}Re]CpTR-Gly was rapidly excreted into urine as its intact structure, [{sup 188}Re]CpTR-COOH was metabolized to more hydrophilic compounds including its glycine conjugate, [{sup 188}Re]CpTR-Gly. Renal excretion of [{sup 188}Re]CpTR-Gly was significantly reduced in probenecid retreated mice. The present studies reinforced that CpTR core remained stable under biological environment. CpTR-COOH was partially recognized as an aromatic acid and was metabolized as such. However, glycine conjugation rendered CpTR-COOH hydrophilic enough to be excreted into urine without further metabolism. These findings suggested that radiolabeling reagents that liberate [{sup 186/188}Re]CpTR-Gly from covalently conjugated {sup 186/188}Re-labeled polypeptides and peptides by the action of renal brush border enzymes would be useful to reduce renal radioactivity levels.

  10. Design and development of Transrapid TR 09; Konstruktion und Entwicklung des Transrapid TR 09

    Energy Technology Data Exchange (ETDEWEB)

    Tum, M. [ThyssenKrupp Transrapid GmbH, Kassel (Germany); Huhn, G. [Deutsche Bahn AG, Berlin (Germany); Harbeke, C. [Nose Applied Intelligence, Zuerich (Switzerland)

    2007-07-01

    Compared with its TR 08 predecessor, the Transrapid 09 had to be modified in many respects in light of the functional specifications of Deutsche Bahn AG and the particular features of the Munich airport link. Thus, in addition to the coach body structure, the access doors and air-conditioning, but also the styling and finally the interior had to be adapted. (orig.)

  11. TR4 nuclear receptor different roles in prostate cancer progression

    Directory of Open Access Journals (Sweden)

    Shin-Jen eLin

    2015-05-01

    Full Text Available Nuclear receptors are important to maintain the tissue homeostasis. Each receptor is tightly controlled and under a very complicated balance. In this review, we summarize the current findings regarding the nuclear receptor TR4 and its role in prostate cancer (PCa progression. In general, TR4 can inhibit the PCa carcinogenesis. However, when PPARγ is knocked out, activation of TR4 can have an opposite effect to promote the PCa carcinogenesis. Clinical data also indicates that higher TR4 expression is found in PCa tissues with high Gleason scores compared to those tissues with low Gleason scores. In vitro and in vivo studies show that TR4 can promote PCa progression. Mechanism dissection indicates that TR4 inhibits PCa carcinogenesis through regulating the tumor suppressor ATM to reduce DNA damages. On the other hand, in the absence of PPARγ, TR4 tends to increase the stem cell population and epithelial-mesenchymal transition (EMT via regulating CCL2, Oct4, EZH2, and miRNA-373-3p expression that results in increased PCa carcinogenesis. In opposition to PCa initiation, TR4 can increase PCa metastasis via modulating the CCL2 signals. Finally, targeting TR4 enhances the chemotherapy and radiation therapy sensitivity in PCa. Together, these data suggest TR4 is a key player to control PCa progression and targeting TR4 with small molecules may provide us a new and better therapy to suppress PCa progression.

  12. A acupuntura vai além da agulha: trajetórias de formação e atuação de acupunturistas

    OpenAIRE

    Marcelo Felipe Nunes; José Roque Junges; Tonantzin Ribeiro Gonçalves; Monique Adriane Motta

    2017-01-01

    Resumo O objetivo deste estudo foi analisar as trajetórias de formação e a atuação profissional de acupunturistas. Trata-se de estudo de natureza qualitativa, baseado no referencial metodológico das narrativas de histórias de vida. Participaram oito profissionais de saúde - cinco homens e três mulheres -, graduados em enfermagem, fisioterapia e medicina, que utilizavam a acupuntura a partir da perspectiva da medicina chinesa (MC) e que responderam a entrevistas semiestruturadas. A análise das...

  13. NR TrA (Nova TrA 2008) monitoring in support of XMM observations

    Science.gov (United States)

    Waagen, Elizabeth O.

    2017-03-01

    Dr. Fred Walter (Stony Brook University) has requested AAVSO observers' assistance in monitoring NR TrA (Nova TrA 2008) in support of upcoming XMM Newton observations. The XMM observations will take place 2017 March 13 06:21 through March 14 10:34 UT. Walter writes: "NR TrA (Nova TrA 2008) is a compact eclipsing system with a 5.5 hour period. It was a normal Fe II nova that, upon reaching quiescence, took on the appearance of a super-soft source in the optical high state, which suggests an extremely high mass accretion rate. The optical spectrum is dominated by hot permitted lines of O VI, N V, C IV, and He II. Some nova-like variables have similar spectra, though generally without the hot emission lines. Primary eclipse is broad - nearly 40% of the orbit - and deeper at shorter wavelengths, which suggests the eclipse of a hot accretion disk. Primary eclipse depth is about 1 mag at V. There appears to be a shallow secondary eclipse.The primary aim [of the XMM observations] is to detect and characterize the eclipse at X-ray and UV wavelengths. We will obtain low cadence BVRI/JHK observations with SMARTS/Andicam. We request AAVSO support to obtain continuous photometric time series simultaneous with the XMM observation. Any filters are acceptable, but standard Johnson B, V or Cousins R, I are preferred. Clear filters are acceptable. Time resolution better than 5 minutes and uncertainties (outside of eclipse) <0.02 mag are preferred. The best ephemeris I have is: minimum light at JD 55956.822 + 0.219109E. This is based on data from 2013-2015." Finder charts with sequence may be created using the AAVSO Variable Star Plotter (https://www.aavso.org/vsp). Observations should be submitted to the AAVSO International Database. See full Alert Notice for more details.

  14. Kallis ID-kaardi trükiliin / Kristina Traks

    Index Scriptorium Estoniae

    Traks, Kristina, 1976-

    2004-01-01

    Ilmunud ka: Delovõje Vedomosti 7. juuli lk. 4. Väidetavalt maksis Eesti riik Trüb Baltic AS-ile ID-kaartide tootmisliini eest üle 100 miljoni krooni enam kui kaartide trükkimise seadmete väärtus. Diagramm: Käive kasvas poole võrra. Vt. samas Villy Paimetsa intervjuud Tarmo Loodusega; Trübil edukas aasta; ID-kaarti kasutab regulaarselt vaid paar protsenti selle omanikest; Villy Paimets. ID-kaarte trükitakse Hansapanga peamajas asuvas üliturvalises keldris

  15. Trækonstruktioner + Tillæg 1, 2005

    DEFF Research Database (Denmark)

    Larsen, H.J.; Riberholt, H.

    Denne anvisning behandler beregning og udførelse af forbindelser i trækonstruktioner i henhold til de normer for bygningskonstruktioner, der udkom i 1998. Materialer generelt og beregning af sædvanlige elementer og konstruktioner er behandlet i SBI-anvisning 193 Trækonstruktioner, Beregning. Anvi...

  16. El tráfico y la trata de mujeres rumanas en la Comunidad de Madrid

    Directory of Open Access Journals (Sweden)

    Silvia Marcu

    2008-01-01

    Full Text Available Tras la caída de los regímenes comunistas y el desplome soviético en el Este de Europa, ocurrieron transformaciones trascendentales. Con el telón de fondo de las transiciones económicas, políticas y sociales, aumentó de la emigración procedente de estos países, hacia los países desarrollados de la UE. Entre los múltiples tipos de emigraciones que se realizaron a partir de los años noventa, apareció en el escenario, la cara más oscura de la inmigración irregular: el tráfico de mujeres. El artículo analiza desde una perspectiva social el fenómeno del tráfico de mujeres procedentes en Rumanía, con destino España, Comunidad de Madrid. Se analiza el contexto, la situación en el origen, la organización del tráfico y la dramática situación de las víctimas en el destino. La autora considera que a pesar de las dificultades a la hora de medir y cuantificar el tráfico de mujeres, es importante realizar la aproximación a la víctima para conocer la realidad sobre esta cara negra y oculta de la inmigración internacional.

  17. Businnes Model of Cors-Tr Tusaga-Aktif

    Science.gov (United States)

    Bakici, S.; Erkek, B.; İlbey, A.; Kulaksiz, E.

    2017-11-01

    CORS-TR (TUSAGA-Aktif (Turkish National Permanent GNSS Network - Active)), serves location information at cm level accuracy in Turkey and TR Northern Cyprus in few seconds, where adequate numbers of GNSS satellites are observed and communication possibilities are present. No ground control points and benchmarks are necessary. There are 146 permanent GNSS stations within the CORS-TR System. Station data are transferred online to the main control center located in the Mapping Department of the General Directorate of Land Registry and Cadastre. CORS-TR System was established in 2008 and has been updated in software, hardware, communication and pricing areas from technical and administrative point of view in order to improve the system and provide better service to the users. Thus, the added value obtained from the CORS-TR System has been increased and contributed to the more efficient use of country resources. In this paper, how the technical, administrative and financial studies in the operation of the CORS-TR System were managed with a sustainable business model, studies for solving problems encountered in operating of the system, the cost / benefit analysis of the system and the sharing of experience gained from the perspective of how web-based applications are managed and the business model of the CORS-TR System are explained in detail.

  18. Spectroscopic studies of Cepheids in Circinus (AV Cir, BP Cir) and Triangulum Australe (R TrA, S TrA, U TrA, LR TrA)

    Science.gov (United States)

    Usenko, I. A.; Kniazev, A. Yu.; Berdnikov, L. N.; Kravtsov, V. V.

    2014-12-01

    Based on high-resolution spectra taken with the 1.9-m telescope of the South African Astronomical Observatory, we have determined the atmospheric parameters and chemical composition for three small-amplitude (AV Cir, BP Cir, and LR TrA), two classical (R TrA and S TrA), and one double-mode (U TrA) Cepheids. The averaged atmospheric parameters have been estimated for three Cepheids (AV Cir, BP Cir, and U TrA) observed at various pulsation phases. In all Cepheids, except U TrA, the metallicity has turned out to be higher than the solar one by 0.1-0.2 dex. The abundances of the key elements of the evolution of yellow supergiants (C, O, Na, Mg, Al) show that these objects have already passed the first dredge-up, while those of the remaining elements are nearly solar. Comparison of our results on the Cepheids from the list (except U TrA) with those of other authors shows significant differences in C and O abundance estimates for AV Cir, R TrA, S TrA, and LR TrA. For AV Cir and BP Cir, the H α line profiles are symmetric but with a slight asymmetry in the core at approximately the same phase near 0{·/ P } 7: on the "blue" side for AV Cir and on the "red" one for BP Cir. BP Cir exhibits a distinct asymmetry in the absorption lines of neutral atoms and ions at various pulsation phases, which can be explained by nonradial first-overtone pulsations. The constancy of the H α absorption line profiles with pulsation phase for AV Cir and BP Cir may suggest the presence of a hydrogen envelope around them. For the double-mode Cepheid U TrA, an asymmetry is observed in the cores of the H α line and the absorption lines of neutral atoms and ions at various pulsation phases, which can be explained by nonradial pulsations in the Cepheid's atmosphere. The absorption lines of neutral atoms and ions of metals in LR TrA closely resemble those in the well-known Cepheid BG Cru: secondary "blue" and "red" components whose line depths vary with pulsation phase are noticeable. This Cepheid

  19. L and P Band MMIC T/R Module Project

    Data.gov (United States)

    National Aeronautics and Space Administration — This proposal is specifically written to address the need for improved L and P band T/R modules for use in active microwave surveillance of earth surface and...

  20. Ka-Band MMIC T/R Module Project

    Data.gov (United States)

    National Aeronautics and Space Administration — This Phase II proposal is presented as the follow on to the Phase I SBIR contract number NNC06CB21C entitled "Ka-band MMIC T/R Module" For active microwave...

  1. L and P Band MMIC T/R Module Project

    Data.gov (United States)

    National Aeronautics and Space Administration — This proposal is specifically written to address the need for improved L and P band T/R modules. The solicitation calls for investigation and development of core...

  2. Ka-Band MMIC T/R Module Project

    Data.gov (United States)

    National Aeronautics and Space Administration — This proposal is specifically written to address the need for improved Ka-band T/R modules. The solicitation calls for investigation and development of core...

  3. A Cuca vai pegar! Medidas do corpo no caldeirão discursivo do medo = Cuca will catch you! body measures in the discursive fear cauldron

    Directory of Open Access Journals (Sweden)

    Nilton Milanez

    2011-07-01

    Full Text Available Este artigo discutirá a lenda da Cuca, bruxa brasileira, que será investigada a partir de três modalidades: a canção infantil “Nana, nenê” de tradição oral, a partitura corporal da Cuca no livro “O Saci” de Monteiro Lobato e a imagem da Cuca em um episódio da edição televisiva do “Sítio do Pica-Pau Amarelo”, em 2001, pela Rede Globo.Tomando os postulados de Michel Foucault na análise do discurso da maneira como a praticamos no Brasil, discutirei a forma como o discurso do medo atravessa o corpo no corpus acima mencionado. Para tanto, identificarei a constituição das monstruosidades, as formas disciplinares que dela surgem, os lugares sociais e históricos que ela evidencia e a produção dos sentidos na rede dessas enunciações.This article will discuss the legend of Cuca, a Brazilian witch, which will be investigated from three modalities: the child song “Nana, nenê” on oral tradition, Cuca’s body composition from the book “O Saci” by Monteiro Lobato and Cuca’s image from a TV series episode called “Sítio do Pica-Pau Amarelo” in 2001 by Rede Globo. Considering the postulates of Michel Foucault within the discourse analysis likewise we practice it in Brazil, I will discuss the way how the discourse about fear crosses the body concerning the corpus below mentioned. Therefore, I will identify the constitution of the monstruosities, the disciplinary way it comes up, the social and historical places it highlights, and the web meaning production of these enunciations.

  4. Detected Timing for Exoplanet TrES-5b. Possible Existence of Exoplanet TrES-5c

    Science.gov (United States)

    Sokov, E. N.; Sokova, I. A.; Dyachenko, V. V.; Rastegaev, D. A.; Rusov, S. A.

    2017-06-01

    In this paper, we present timing variations detected for the TrES-5b exoplanet. To obtain necessary photometric data for this exoplanet, we have organized an international campaign for exoplanet searching based on the Transit Timing Variation (TTV) method. We managed to collect N light curves for TrEs-5b. On the basis of the obtained data, we detected timing variations with the period P ≍ 100 days. We carried out the N-body modelling by means of the three-body problem. We detected a perturbation of TrES-5b which can be caused by a second exoplanet in the TrES-5 system. We calculated possible masses and resonances of the objects: M ˜ 0.24 Mjup on the 1:2 Resonance and M ˜ 3.15 Mjup on the 1:3 Resonance.

  5. A ironia trágica de Machado de Assis

    Directory of Open Access Journals (Sweden)

    Patrick Pessoa

    2007-04-01

    Full Text Available O texto defende, a partir de uma análise das Memórias póstumas de Brás Cubas, de Machado de Assis, que a ironia do autor deve ser lida como uma espécie de ironia trágica. A fundamentação dessa hipótese é realizada em três etapas: na primeira, apresenta-se o conceito de ironia trágica, como tematizado por Christoph Menke e Wayne Booth; na segunda, mostra-se como a noção de ironia trágica permite compreender a célebre definição de Lukács de que “a ironia é a objetividade do romance”; finalmente, o texto discute como o conceito de ironia trágica ao mesmo tempo esclarece e subverte as interpretações tradicionais da obra de Machado de Assis.

  6. Ecological risk assessment of a coastal zone in Southern Vietnam: Spatial distribution and content of heavy metals in water and surface sediments of the Thi Vai Estuary and Can Gio Mangrove Forest.

    Science.gov (United States)

    Costa-Böddeker, Sandra; Hoelzmann, Philipp; Thuyên, Lê Xuân; Huy, Hoang Duc; Nguyen, Hoang Anh; Richter, Otto; Schwalb, Antje

    2017-01-30

    Enrichment of heavy metals was assessed in the Thi Vai Estuary and in the Can Gio Mangrove Forest (SE, Vietnam). Cd, Co, Cr, Cu, Mn, Ni, Pb and Zn contents in water and in sediments were measured. Total organic carbon, nitrogen, phosphorus and C/N ratios were determined. Cu and Cr values were higher than threshold effect level of toxicity, while Ni exceeded probable effect level, indicating the risk of probable toxicity effects. Enrichment factors (EF), contamination factor (CF) and Geo-accumulation index (I-geo) were determined. CF reveals moderate to considerable pollution with Cr and Ni. EF suggests anthropogenic sources of Cr, Cu and Ni. I-geo indicates low contamination with Co, Cu and Zn and moderate contamination with Cr and Ni. Overall metal contents were lower than expected for this highly industrialized region, probably due to dilution, suggesting that erosion rates and hydrodynamics may also play a role in metal contents distribution. Copyright © 2016 Elsevier Ltd. All rights reserved.

  7. Trópicos tristes y paradisíacos

    Directory of Open Access Journals (Sweden)

    Vicente Francisco Torres

    2011-09-01

    Full Text Available Este artículo continúa las indagatorias del autor sobre la visión de la selva que han dado los hombres de letras en Hispanoamérica. Como un estudio de esta naturaleza no estaría completo sin la presencia de los naturalistas, que coincidieron en equiparar el trópico con el paraíso, el presente trabajo entrega una lectura de ellos y le da cita al antropólogo Claude Lévy-Strauss, quien fue una voz discordante en la visión paradisíaca del trópico.

  8. INTRACELLULAR Ca2+ HOMEOSTASIS

    Directory of Open Access Journals (Sweden)

    Shahdevi Nandar Kurniawan

    2015-01-01

    Full Text Available Ca2+ signaling functions to regulate many cellular processes. Dynamics of Ca2+ signaling or homeostasis is regulated by the interaction between ON and OFF reactions that control Ca2+ flux in both the plasma membrane and internal organelles such as the endoplasmic reticulum (ER and mitochondria. External stimuli activate the ON reactions, which include Ca2+ into the cytoplasm either through channels in the plasma membrane or from internal storage like in ER. Most of the cells utilize both channels/sources, butthere area few cells using an external or internal source to control certain processes. Most of the Ca2+ entering the cytoplasm adsorbed to the buffer, while a smaller part activate effect or to stimulate cellular processes. Reaction OFF is pumping of cytoplasmic Ca2+ using a combination mechanism of mitochondrial and others. Changes in Ca2+ signal has been detected in various tissues isolated from animals induced into diabetes as well as patients with diabetes. Ca2+ signal interference is also found in sensory neurons of experimental animals with diabetes. Ca2+ signaling is one of the main signaling systems in the cell.

  9. Understanding TR binding to pMHC complexes: how does a TR scan many pMHC complexes yet preferentially bind to one.

    Directory of Open Access Journals (Sweden)

    Javed Mohammed Khan

    Full Text Available Understanding the basis of the binding of a T cell receptor (TR to the peptide-MHC (pMHC complex is essential due to the vital role it plays in adaptive immune response. We describe the use of computed binding (free energy (BE, TR paratope, pMHC epitope, molecular surface electrostatic potential (MSEP and calculated TR docking angle (θ to analyse 61 TR/pMHC crystallographic structures to comprehend TR/pMHC interaction. In doing so, we have successfully demonstrated a novel/rational approach for θ calculation, obtained a linear correlation between BE and θ without any "codon" or amino acid preference, provided an explanation for TR ability to scan many pMHC ligands yet specifically bind one, proposed a mechanism for pMHC recognition by TR leading to T cell activation and illustrated the importance of the peptide in determining TR specificity, challenging the "germline bias" theory.

  10. Integrated L-Band T/R Module Project

    Data.gov (United States)

    National Aeronautics and Space Administration — The goal of this Phase II project is to deliver an integrated L-band transmit/receive (T/R) module which will be fabricated from a GaAs-based combined HBT/PHEMT...

  11. Korroderede trådbindere i murværk

    DEFF Research Database (Denmark)

    Hansen, Klavs Feilberg

    Denne SBi-anvisning omdhandler korroderede trådbindere i hule mure og skalmure, og den nedstyrtningsfare som korrosionen kan medføre. Anvisningen beskriver metoder til at undersøge og vurdere problemet, og hvordan man kan eftermontere nye bindere. Anvisningen er rettet mod rådgivende ingeniører....

  12. Ajast ja arust trükikoda Kultuuritehases / Merit Kask

    Index Scriptorium Estoniae

    Kask, Merit

    2006-01-01

    Tallinna Kultuuritehase trükikojas toimunud kõrgtrükitehnikas käsilao õpikojast. Juhendaja inglise kunstnik Pete Nevin, osa võtsid Eesti Kunstiakadeemia tudengid (nimed), kelle tehtud tööd on samas näitusel

  13. Recurrent nodular haemangiomas in Klippel-Trénaunay syndrome

    DEFF Research Database (Denmark)

    Illum, N; Winther Nielsen, H; Guldhammer Skov, B

    1992-01-01

    . Microscopic examination of these elements showed haemangiomas with capillaries, cavernous channels and lymphangiomatous components. The benign nature of transient nodular elements located to the trunk and the lack of associated visceral vascular malformations in the Klippel-Trénaunay syndrome are documented....

  14. Trükitud majad : korterelamu Pariisis / Triin Ojari

    Index Scriptorium Estoniae

    Ojari, Triin, 1974-

    1998-01-01

    Tänaste fassaadide keerukusest, paindlikkusest, vastuolulisusest. Trükitud klaasist fassaadi näiteks toodud elamu uue Pariisi Rahvusraamatukogu kõrval. Hoone pinna moodustavad suured värvitrükis klaaspaneelid, molle motiivid on laenatud renessanssmaalidelt. Katkematu lindina jooksevad ümber maja etteulatuvad metallrõdud.

  15. Counsellors Respond to the DSM-IV-TR

    Science.gov (United States)

    Strong, Tom; Gaete, Joaquin; Sametband, Ines N.; French, Jared; Eeson, Jen

    2012-01-01

    The Diagnostic and Statistical Manual of Mental Disorders (DSM-IV-TR) is an administrative fact for many counsellors. This psychiatric approach to formulating client concerns runs counter to those used by counsellors of many approaches (e.g., systemic, feminist). Using an online survey of counsellors (N = 116), invited contributions to a website…

  16. TrED: the Trichophyton rubrum Expression Database

    Directory of Open Access Journals (Sweden)

    Liu Tao

    2007-07-01

    Full Text Available Abstract Background Trichophyton rubrum is the most common dermatophyte species and the most frequent cause of fungal skin infections in humans worldwide. It's a major concern because feet and nail infections caused by this organism is extremely difficult to cure. A large set of expression data including expressed sequence tags (ESTs and transcriptional profiles of this important fungal pathogen are now available. Careful analysis of these data can give valuable information about potential virulence factors, antigens and novel metabolic pathways. We intend to create an integrated database TrED to facilitate the study of dermatophytes, and enhance the development of effective diagnostic and treatment strategies. Description All publicly available ESTs and expression profiles of T. rubrum during conidial germination in time-course experiments and challenged with antifungal agents are deposited in the database. In addition, comparative genomics hybridization results of 22 dermatophytic fungi strains from three genera, Trichophyton, Microsporum and Epidermophyton, are also included. ESTs are clustered and assembled to elongate the sequence length and abate redundancy. TrED provides functional analysis based on GenBank, Pfam, and KOG databases, along with KEGG pathway and GO vocabulary. It is integrated with a suite of custom web-based tools that facilitate querying and retrieving various EST properties, visualization and comparison of transcriptional profiles, and sequence-similarity searching by BLAST. Conclusion TrED is built upon a relational database, with a web interface offering analytic functions, to provide integrated access to various expression data of T. rubrum and comparative results of dermatophytes. It is devoted to be a comprehensive resource and platform to assist functional genomic studies in dermatophytes. TrED is available from URL: http://www.mgc.ac.cn/TrED/.

  17. Desulfurizing Ability of the CaOsatd.-CaCl2-CaF2 Slags

    Science.gov (United States)

    Liu, Jiazhan; Kobayashi, Yoshinao

    2017-04-01

    Desulfurizing ability of the CaO-CaCl2-CaF2 slags saturated with CaO has been investigated from the viewpoint of the sulfide capacity and CaO solubility. The CaO-CaCl2-CaF2 slags containing small amounts of Cu2O and CaS were inserted in a CaO crucible with metallic copper. The CaO crucible was sealed in a nickel holder to prevent the evaporation of CaCl2, then heated up and kept at temperatures from 1573 K (1300 °C) to 1673 K (1400 °C) for 24 hours, which enabled the system inside the CaO crucible to reach the equilibrium. As expected, the sulfide capacity derived from the data obtained as well as CaO solubility of the slag increase with an increase in temperature at a constant ratio of CaCl2/CaF2. The solubility of CaO increases by the replacement of CaF2 with CaCl2, whereas the sulfide capacity slightly decreases and the activity coefficient of CaS ( γ CaS) increases. This suggests that CaF2 has stronger interaction with CaS than CaCl2. The sulfur distribution ratio between carbon-saturated iron melts and the CaO-CaCl2 slag has been calculated to be about 10 000 at 1573 K (1300 °C) using the sulfide capacity obtained, which value is still large enough even with the replacement of CaF2 by CaCl2.

  18. EBCO Technologies TR Cyclotrons, Dynamics, Equipment, and Applications

    Science.gov (United States)

    Johnson, R. R.; Erdman, K. L.; Gyles, Wm.; Burbee, J.; VanLier, E.; Kovacs, M.; Perron, F.

    2003-08-01

    The Ebco Technologies TR cyclotrons have a common parent in the 500 MeV negative ion cyclotron at TRIUMF in Vancouver. As such, the TR cyclotrons have features that can be adapted for specific application. The cyclotron design is modularized into ion source and injection system, central region and then extraction. The cyclotron ion source is configured for cyclotron beam currents ranging from 50 microAmps to 2 milliAmps. The injection line can be operated in either continuous (CW) or in pulsed mode. The center region of the cyclotron is configured to match the ion source configuration. The extracted beams are directed either to a local target station or to beam lines and thence to target stations. There has been development both in solid, liquid and gas targets. There has been development in radioisotope handling techniques, target material recovery and radiochemical synthesis.

  19. Paracoccidioidomicose palpebral: relato de três casos

    Directory of Open Access Journals (Sweden)

    Rocha Guilherme José Nunes Marques

    2002-01-01

    Full Text Available Objetivo: Relato clínico-patológico de três casos de paracoccidioidomicose multifocal comprometendo pálpebra e secundariamente a conjuntiva em dois deles. Métodos: Revisão dos prontuários e preparações histopatológicas obtidas das biópsias. Resultado: O estudo histopatológico realizado nos três casos, evidenciou reação inflamatória de tipo granulomatoso. A coloração pelo método de Grocott demonstrou a presença do Paracoccidioides brasiliensis. Conclusão: É importante a inclusão da paracoccidioidomicose no diagnóstico diferencial de lesões tumorais acometendo as pálpebras.

  20. Teale CA. Counties

    Data.gov (United States)

    California Department of Resources — California Spatial Information System (CaSIL) is a project designed to improve access to geo-spatial and geo-spatial related data information throughout the state of...

  1. Efficient inverse position transformation for TR 4000S robot manipulator

    Directory of Open Access Journals (Sweden)

    Kesheng Wang

    1989-04-01

    Full Text Available An efficient method is developed for computing the inverse kinematic position solution with a closed form for the TR 4000S spray painting robot manipulator with five degrees of freedom and non-spherical wrist construction. The inverse kinematic problem is defined as the transformation from Cartesian space to the joint space. The solution is based on the geometrical separation of the arm and wrist of a robot manipulator and shows that it is very systematic, efficient and easily derived.

  2. Deconstructing the DSM-IV-TR: a critical perspective.

    Science.gov (United States)

    Warelow, Philip; Holmes, Colin A

    2011-12-01

    This paper examines and offers a critique of the Diagnostic and Statistical Manual of Mental Disorders, 4th Edition, Text Revision (DSM-IV-TR), underlying principles and assumptions, and the nature and consequences of its nosological framework. The reason for this critique is to look at the rationale for some of the diagnostic categories and also why some categories are retained, including some of the long-standing diagnostic groups, such as schizophrenia. It is not the intention here to rehearse the problems of biological psychiatric thinking, nor argue the strengths and weaknesses of the DSM-IV-TR in its definitions and descriptions of particular syndromes and illnesses. The ideas presented here derive from a range of previous research that argued that the DSM-IV-TR colludes in a system of psychiatric care in which all people, by virtue of characteristically human foibles and idiosyncrasies, are potentially classifiable into a variety of diagnostic mental health categories. In the present study, it was argued that because of resource constraints, professional dispute, and public concern, the major criterion for attracting a formal diagnosis is not classifiability according to the DSM-IV-TR, but rather, that of 'social risk', defined in terms of risk to oneself and/or others and embodying obvious social control functions. Here, we expand and develop some of these ideas, and relate them more specifically to insights offered by critical or deconstructive psychology and the development of the forthcoming the DSM-V. © 2011 The Authors. International Journal of Mental Health Nursing © 2011 Australian College of Mental Health Nurses Inc.

  3. Trauma Informed Guilt Reduction (TrIGR) Intervention

    Science.gov (United States)

    2016-10-01

    AWARD NUMBER: W81XWH-15-1-0331 TITLE: Trauma -Informed Guilt Reduction (TrIGR) Intervention PRINCIPAL INVESTIGATOR: Christy Capone, PhD...ABOVE ADDRESS. 1. REPORT DATE October 2016 2. REPORT TYPE Annual 3. DATES COVERED 30 Sep 2015 - 29 Sep 2016 4. TITLE AND SUBTITLE Trauma -Informed...disorders. There is a pressing need for effective treatments targeting transdiagnostic mechanisms such as guilt. We developed Trauma Informed Guilt

  4. Forebyggende fysisk træning til ældre

    DEFF Research Database (Denmark)

    Termansen, Tina; Scavenius Sonne-Schmidt, Christoffer

    Vores fysiske funktionsevne bliver ringere med alderen. Det er en væsentlig årsag til, at ældre har en øget risiko for at komme ud for ulykker på grund af fald. Denne rapport præsenterer resultaterne fra en undersøgelse om faldforebyggende træning til ældre hjemmeboende borgere over 65 år, bosat ...

  5. La Universidad de Costa Rica en tránsito

    Directory of Open Access Journals (Sweden)

    Badilla Saxe, Eleonora

    2012-02-01

    Full Text Available Resumen. La Universidad de Costa Rica en Tránsito es un artículo que pretende dar cuenta del tránsito que ha iniciado la institución en su camino hacia la transdisciplinariedad. Se presenta, en primera instancia, un contexto histórico y referentes teóricos que apuntan a que la Universidad en el Siglo XXI debe iniciar un tránsito, por una parte, de regreso a reflejar el significado de su origen: UNIVERSUS-A-UM (“todo”, “entero”, “universal” superando fragmentaciones y departamentalizaciones y, por otro, hacia una visión transdisciplinar, un pensamiento complejo en sintonía con las realidades biológicas, sociales y culturales del mundo en el siglo XXI. Y, ya que la transdisciplinariedad no se puede llevar a cabo más que en la acción y en la interacción con otros, se reporta sobre una serie de estrategias interconectadas que se están promoviendo Universidad de Costa Rica para ayudar a la institución a iniciar ese tránsito.Abstract. University of Costa Rica in Transit is an article that reports on the journey the institution has started on its path towards transdisciplinarity. On one way, back to the origen: universus (all, whole, universal, overcoming fragmentation and departamentalization. On the other towards a transdisciplinary vision and complex thinking in accordance with the new biological, social and cultural realities of our world. Interactive and interrelated strategies that are currently beeing promoted to stimulate the institution towards transdisciplinarity are reported here. It is important to remember that transdisciplinarity can only be reflected in action, and in the interaction with others.

  6. UWB channel estimation using new generating TR transceivers

    Science.gov (United States)

    Nekoogar, Faranak [San Ramon, CA; Dowla, Farid U [Castro Valley, CA; Spiridon, Alex [Palo Alto, CA; Haugen, Peter C [Livermore, CA; Benzel, Dave M [Livermore, CA

    2011-06-28

    The present invention presents a simple and novel channel estimation scheme for UWB communication systems. As disclosed herein, the present invention maximizes the extraction of information by incorporating a new generation of transmitted-reference (Tr) transceivers that utilize a single reference pulse(s) or a preamble of reference pulses to provide improved channel estimation while offering higher Bit Error Rate (BER) performance and data rates without diluting the transmitter power.

  7. TrSDB: a proteome database of transcription factors

    Science.gov (United States)

    Hermoso, Antoni; Aguilar, Daniel; Aviles, Francesc X.; Querol, Enrique

    2004-01-01

    TrSDB—TranScout Database—(http://ibb.uab.es/trsdb) is a proteome database of eukaryotic transcription factors based upon predicted motifs by TranScout and data sources such as InterPro and Gene Ontology Annotation. Nine eukaryotic proteomes are included in the current version. Extensive and diverse information for each database entry, different analyses considering TranScout classification and similarity relationships are offered for research on transcription factors or gene expression. PMID:14681387

  8. Fysisk træning på psykomotorikuddannelsen

    DEFF Research Database (Denmark)

    Flammild, Manja

    2012-01-01

    rolle i sundhedsfremme og forebyggelse, og ønsket er, at viden fra faget kan rettes mod forskellige målgrupper? Problemformuleringen undersøges med fokus på forskellige målgrupper, om end at læringsmålet i fysisk træning primært er rettet mod voksne inden for normalområdet. Der kigges særligt på...

  9. Marinization concept for the TRICEPT TR600 robot

    Energy Technology Data Exchange (ETDEWEB)

    Meyer, A.; Aust, E.; Niemann, H.R.; Santos, J.F. dos [GKSS-Forschungszentrum Geesthacht GmbH (Germany). Inst. fuer Materialforschung; Hammerin, R.; Neumann, K.E. [Neos Robotics AB, Taeby (Sweden); Gibson, D. [National Hyperbaric Centre, Aberdeen (United Kingdom)

    1998-11-01

    The need for automated welding repair systems of marine structures, ship hulls and nuclear installations had lead to an increasing demand for subsea robots. Considering the application of friction welding to perform underwater repairs, a TRICEPT TR600 robot has been identified as the most suitable system to withstand the high reaction forces characteristic of this process. This study reviews initially the research and development work carried out at GKSS to modify and test a Siemens-MANUTEC robot. After a description of the TRICEPT TR600 robot a marinization concept is presented and discussed in detail. Problems of galvanic corrosion in seawater are addressed in a separate chapter. The deflection of the robot in subsea water currents is estimated with a worst-case calculation. (orig.) [Deutsch] Der Wunsch, Roboter auch unter Wasser einsetzen zu koennen, waechst mit steigendem Interesse nach automatisierten Schweissverfahren fuer Reparaturen an marinen Bauwerken, Schiffsruempfen und in Kernenergieanlagen. Fuer den Einsatz von Reibschweissverfahren fuer diese Reparaturen wurde der TRICEPT TR600-Roboter ausgewaehlt, da dieser auch den charakteristisch hohen Prozesskraeften widerstehen kann. Die notwendigen Modifikationen und Pruefungen werden beispielhaft anhand des bei der GKSS modifizierten Siemens-MANUTEC-Roboters vorgestellt. Nach einer Beschreibung des TRICEPT-Roboters werden die notwendigen Umbaumassnahmen detailliert dargestellt und diskutiert. Auf die Problematik der galvanischen Korrosion in Seewasser wird in einem gesonderten Kapitel naeher eingegangen. Zusaetzlich wird eine moegliche Ablenkung des Roboters durch Wasserstroemung ueberschlaegig berechnet. (orig.)

  10. Fermentation of Foc TR4-infected bananas and Trichoderma spp.

    Science.gov (United States)

    Yang, J; Li, B; Liu, S W; Biswas, M K; Liu, S; Wei, Y R; Zuo, C W; Deng, G M; Kuang, R B; Hu, C H; Yi, G J; Li, C Y

    2016-10-17

    Fusarium wilt (also known as Panama disease) is one of the most destructive banana diseases, and greatly hampers the global production of bananas. Consequently, it has been very detrimental to the Chinese banana industry. An infected plant is one of the major causes of the spread of Fusarium wilt to nearby regions. It is essential to develop an efficient and environmentally sustainable disease control method to restrict the spread of Fusarium wilt. We isolated Trichoderma spp from the rhizosphere soil, roots, and pseudostems of banana plants that showed Fusarium wilt symptoms in the infected areas. Their cellulase activities were measured by endoglucanase activity, β-glucosidase activity, and filter paper activity assays. Safety analyses of the Trichoderma isolates were conducted by inoculating them into banana plantlets. The antagonistic effects of the Trichoderma spp on the Fusarium pathogen Foc tropical Race 4 (Foc TR4) were tested by the dual culture technique. Four isolates that had high cellulase activity, no observable pathogenicity to banana plants, and high antagonistic capability were identified. The isolates were used to biodegrade diseased banana plants infected with GFP-tagged Foc TR4, and the compost was tested for biological control of the infectious agent; the results showed that the fermentation suppressed the incidence of wilt and killed the pathogen. This study indicates that Trichoderma isolates have the potential to eliminate the transmission of Foc TR4, and may be developed into an environmentally sustainable treatment for controlling Fusarium wilt in banana plants.

  11. Canal de Mensagens de Trânsito

    Directory of Open Access Journals (Sweden)

    Egon Cervieri Guterres

    2010-05-01

    Full Text Available Informe Setorial: O Canal de Mensagens de Trânsito (Traffic Message Channel - TMC é uma padronização internacional para a distribuição de informações (conteúdo sobre a situação do trânsito urbano local e inter-regional, em tempo real, de forma eletrônica e diretamente aos motoristas. Traz para os cidadãos, em mensagens eletrônicas simples e acessíveis, informações sobre congestionamentos, acidentes, obras na pista, problemas climáticos (alagamentos, asfalto escorregadio, nevoeiro... e desvios de rota, além de muitos outros eventos de trânsito, oferecendo, em linguagem padronizada de fácil compreensão, a descrição do incidente, área de efeito, duração esperada e, quando possível, caminhos alternativos para evitá-lo.

  12. DW4TR: A Data Warehouse for Translational Research.

    Science.gov (United States)

    Hu, Hai; Correll, Mick; Kvecher, Leonid; Osmond, Michelle; Clark, Jim; Bekhash, Anthony; Schwab, Gwendolyn; Gao, De; Gao, Jun; Kubatin, Vladimir; Shriver, Craig D; Hooke, Jeffrey A; Maxwell, Larry G; Kovatich, Albert J; Sheldon, Jonathan G; Liebman, Michael N; Mural, Richard J

    2011-12-01

    The linkage between the clinical and laboratory research domains is a key issue in translational research. Integration of clinicopathologic data alone is a major task given the number of data elements involved. For a translational research environment, it is critical to make these data usable at the point-of-need. Individual systems have been developed to meet the needs of particular projects though the need for a generalizable system has been recognized. Increased use of Electronic Medical Record data in translational research will demand generalizing the system for integrating clinical data to support the study of a broad range of human diseases. To ultimately satisfy these needs, we have developed a system to support multiple translational research projects. This system, the Data Warehouse for Translational Research (DW4TR), is based on a light-weight, patient-centric modularly-structured clinical data model and a specimen-centric molecular data model. The temporal relationships of the data are also part of the model. The data are accessed through an interface composed of an Aggregated Biomedical-Information Browser (ABB) and an Individual Subject Information Viewer (ISIV) which target general users. The system was developed to support a breast cancer translational research program and has been extended to support a gynecological disease program. Further extensions of the DW4TR are underway. We believe that the DW4TR will play an important role in translational research across multiple disease types. Copyright © 2011 Elsevier Inc. All rights reserved.

  13. O rato Sniffy vai à escola

    Directory of Open Access Journals (Sweden)

    Gerson Yukio Tomanari

    Full Text Available Sniffy Pro - O Rato Virtual é um programa de computador, acompanhado de um manual de laboratório, que tem como proposta servir de recurso didático aplicado ao ensino introdutório de Análise Experimental do Comportamento, em especial às atividades práticas normalmente desenvolvidas em laboratórios de condicionamento operante que empregam ratos como sujeitos e caixas de Skinner como equipamento experimental. O objetivo do presente artigo é analisar Sniffy Pro no tocante aos aspectos concernentes à sua pertinência e adequação como material de ensino. Segundo os autores de Sniffy Pro, este aplicativo seria atraente pelo que representa em termos de tecnologia informatizada, pelo seu baixo custo, pela sua facilidade de uso e pela economia de tempo que propicia. Entretanto, tomados vários aspectos da concepção de Sniffy Pro, seu conteúdo e estrutura, bem como alguns aspectos éticos relacionados, demonstra-se que a sua utilização como recurso didático deve muito provavelmente ocorrer às custas de prejuízos na formação científica do estudante de Psicologia.

  14. Tuomiokapitulin vai seurakuntalaisten valtaa? : Tapaustutkimus kirkkoherranvaaliprosessista

    OpenAIRE

    Tuisku, Jaakko

    2014-01-01

    The authority of a Cathedral chapter or of the parishioners? A case study of election process for vicars. A vicar s post as a leader in a Finnish Evangelical-Lutheran parish is a significant post. Selection to this position has been a multi-phased process since the Middle Ages. The election of a vicar has been an important event for parishioners for centuries. At the turn of the decade (2010s), the election system for vicars came under strong pressure for change. In this study the e...

  15. Biomarker CA125

    DEFF Research Database (Denmark)

    Kargo, Anette Stolberg

    Background: The majority of patients with ovarian cancer (OC) are diagnosed with advanced disease (70-80 %) and will experience disease relapse with only limited curative potential. Early initiation of relapse treatment based on rising CA125 alone does not improve survival. Increasing CA125 can...... be detected months before symptoms arise and recurrence is visible on imaging. Therefore, biochemical detection of potential relapse by CA125 assessment can cause significant distress. A decision aid (DA) is a tool that provides information and describes advantages and disadvantages of a specific intervention...... patient organisations and cancer societies. First, a focus group of seven former OC patients was performed followed by a quantitative rating of the DA pilot version. The DA was adapted accordingly and then tested in 14 OC patients with recurrence using a structured interview guide (alpha testing). A final...

  16. Trængselskommissionen - og hva' så?

    DEFF Research Database (Denmark)

    Jespersen, Per Homann

    2014-01-01

    Trængselskommissionens betænkning udkom for snart et år siden. Den indeholder en masse gode forslag til forbedring af trafiksystemet i Hovedstadsområdet. Den er også på mange måder nytænkende i forhold til mål og midler, der er nødvendige for at mobiliteten i et storbyområde kan opretholdes og ud......, hvad jeg mener, er de vigtigste resultater, og gennem nogle eksempler på, hvordan kommissionen har arbejdet, kommer jeg med mine personlige refleksioner om samspillet mellem politikerne, embedsværket og kommissioner...

  17. Berenice: la mujer, figura trágico-fantasmal

    OpenAIRE

    Alvarado Vega, Óscar Gerardo

    2009-01-01

    La mujer desempeña un rol fundamental en nuestra sociedad. Sin embargo, en diferentes discursos, del cual la literatura es claramente uno, su condición de alienación o de marginalidad la ha llevado a un plano secundario. Extendiendo tal apreciación a un relato de Poe, creemos que ella ha sido abordada, citando el texto "Berenice" en particular, de una manera más bien simbólica, poseedora (¿o receptora?) de un carácter trágico, propio de su condición como mujer.

  18. Datasets from an interaction proteomics screen for substrates of the SCFβTrCP ubiquitin ligase

    NARCIS (Netherlands)

    Magliozzi, Roberto; Peng, Mao; Mohammed, Shabaz; Guardavaccaro, Daniele; Heck, Albert J R; Low, Teck Yew

    2015-01-01

    An affinity purification-mass spectrometry (AP-MS) method was employed to identify novel substrates of the SCFβTrCP ubiquitin ligase. A FLAG-HA tagged version of the F-box protein βTrCP2, the substrate recognition subunit of SCFβTrCP, was used as bait. βTrCP2 wild type and the two mutants

  19. The roles of testicular nuclear receptor 4 (TR4 in male fertility-priapism and sexual behavior defects in TR4 knockout mice

    Directory of Open Access Journals (Sweden)

    Bao Bo-Ying

    2011-10-01

    Full Text Available Abstract Background Successful reproductive efforts require the establishment of a situation favorable for reproduction that requires integration of both behavior and internal physiological events. TR4 nuclear receptor is known to be involved in male fertility via controlling spermatogenesis, yet its roles in regulating other biological events related to reproduction have not been completely revealed. Methods Male TR4 knockout (TR4-/- and wild type mice were used for the sexual behavior and penile dysfunction studies. Mice were sacrificed for histological examination and corresponding genes profiles were analyzed by quantitative RT-PCR. Reporter gene assays were performed. Results We describe an unexpected finding of priapism in TR4-/- mice. As a transcriptional factor, we demonstrated that TR4 transcriptionally modulates a key enzyme regulating penis erection and neuronal nitric oxide synthese NOS (nNOS. Thereby, elimination of TR4 results in nNOS reduction in both mRNA and protein levels, consequently may lead to erectile dysfunction. In addition, male TR4-/- mice display defects in sexual and social behavior, with increased fear or anxiety, as well as reduced mounting, intromission, and ejaculation. Reduction of ER alpha, ER beta, and oxytocin in the hypothalamus may contribute to defects in sexual behavior and stress response. Conclusions Together, these results provide in vivo evidence of important TR4 roles in penile physiology, as well as in male sexual behavior. In conjunction with previous finding, TR4 represents a key factor that controls male fertility via regulating behavior and internal physiological events.

  20. Posttranslational Control of ALA Synthesis Includes GluTR Degradation by Clp Protease and Stabilization by GluTR-Binding Protein.

    Science.gov (United States)

    Apitz, Janina; Nishimura, Kenji; Schmied, Judith; Wolf, Anja; Hedtke, Boris; van Wijk, Klaas J; Grimm, Bernhard

    2016-04-01

    5-Aminolevulinic acid (ALA) is the first committed substrate of tetrapyrrole biosynthesis and is formed from glutamyl-tRNA by two enzymatic steps. Glutamyl-tRNA reductase (GluTR) as the first enzyme of ALA synthesis is encoded by HEMA genes and tightly regulated at the transcriptional and posttranslational levels. Here, we show that the caseinolytic protease (Clp) substrate adaptor ClpS1 and the ClpC1 chaperone as well as the GluTR-binding protein (GBP) interact with the N terminus of GluTR Loss-of function mutants of ClpR2 and ClpC1 proteins show increased GluTR stability, whereas absence of GBP results in decreased GluTR stability. Thus, the Clp protease system and GBP contribute to GluTR accumulation levels, and thereby the rate-limiting ALA synthesis. These findings are supported with Arabidopsis (Arabidopsis thaliana) hema1 mutants expressing a truncated GluTR lacking the 29 N-terminal amino acid residues of the mature protein. Accumulation of this truncated GluTR is higher in dark periods, resulting in increased protochlorophyllide content. It is proposed that the proteolytic activity of Clp protease counteracts GBP binding to assure the appropriate content of GluTR and the adequate ALA synthesis for chlorophyll and heme in higher plants. © 2016 American Society of Plant Biologists. All Rights Reserved.

  1. ”Tusinder af vingeskudte Trækfugle”. Soldatergrave og dansk-franske erindringssteder 1915-1925 ca.

    Directory of Open Access Journals (Sweden)

    Ning de Coninck-Smith

    2012-05-01

    Full Text Available Thousands of Wing-Shot Migratory Birds. Soldier Graves and Danish-French Places of Remembrance Approx. 1915-1925During the months following the end of the First World War in November 1918, some 100,000 prisoners of war passed through Denmark on their way home from the camps on the Eastern Front. Some did not make it all the way, but died from exhaustion and the Spanish flu during their stay in Denmark. The present article deals with the part that these dead soldiers came to play in the formation of a remembrance culture in a country which had not itself taken part in the war. More specifically, it deals with the monuments which a small group of nationally-conservative men and women with ties to the armed forces and the social elite erected between 1919 and 1925 in remembrance of the dead French soldiers. To their minds, France had been the sole serious ally in the struggle for the return of North Schleswig to Denmark. For that reason, they were also behind two monuments in France to commemorate the fallen Danish-minded Schleswigers and the fallen Danes of the French Foreign Legion. Their national-conservative engagementand criticism of the policy of neutrality pursued during the war by the Danish government largely determined the creation and the form of the cemeteries.

  2. Topological organization of CA3-to-CA1 excitation.

    Science.gov (United States)

    Hongo, Yoshie; Ogawa, Koichi; Takahara, Yuji; Takasu, Keiko; Royer, Sebastien; Hasegawa, Minoru; Sakaguchi, Gaku; Ikegaya, Yuji

    2015-09-01

    The CA1-projecting axons of CA3 pyramidal cells, called Schaffer collaterals, constitute one of the major information flow routes in the hippocampal formation. Recent anatomical studies have revealed the non-random structural connectivity between CA3 and CA1, but little is known regarding the functional connectivity (i.e. how CA3 network activity is functionally transmitted downstream to the CA1 network). Using functional multi-neuron calcium imaging of rat hippocampal slices, we monitored the spatiotemporal patterns of spontaneous CA3 and CA1 burst activity under pharmacological GABAergic blockade. We found that spatially clustered CA3 activity patterns were transformed into layered CA1 activity sequences. Specifically, synchronized bursts initiated from multiple hot spots in CA3 ensembles, and CA1 neurons located deeper in the pyramidal cell layer were recruited during earlier phases of the burst events. The order of these sequential activations was maintained across the bursts, but the sequence velocity varied depending on the inter-burst intervals. Thus, CA3 axons innervate CA1 neurons in a highly topographical fashion. © 2015 Federation of European Neuroscience Societies and John Wiley & Sons Ltd.

  3. Macrolide resistance gene erm(TR) and erm(TR)-carrying genetic elements in Streptococcus agalactiae: characterization of ICESagTR7, a new composite element containing IMESp2907.

    Science.gov (United States)

    Mingoia, Marina; Morici, Eleonora; Marini, Emanuela; Brenciani, Andrea; Giovanetti, Eleonora; Varaldo, Pietro E

    2016-03-01

    The objective of this study was to investigate macrolide-resistant Streptococcus agalactiae isolates harbouring erm(TR), an erm(A) gene subclass, with emphasis on their erm(TR)-carrying genetic elements. Four erm(TR)-carrying elements have been described to date: three closely related (ICE10750-RD.2, Tn1806 and ICESp1108) in Streptococcus pyogenes, Streptococcus pneumoniae and S. pyogenes, respectively; and one completely different (IMESp2907, embedded in ICESp2906 to form ICESp2905) in S. pyogenes. Seventeen macrolide-resistant erm(TR)-positive S. agalactiae isolates were phenotypically and genotypically characterized. Their erm(TR)-carrying elements were explored by analysing the distinctive recombination genes of known erm(TR)-carrying integrative and conjugative elements (ICEs) and by PCR mapping. The new genetic context and organization of IMESp2907 in S. agalactiae were explored using several experimental procedures and in silico analyses. Five isolates harboured ICE10750-RD.2/Tn1806, five isolates harboured ICESp1108 and five isolates bore unknown erm(TR)-carrying elements. The remaining two isolates, exhibiting identical serotypes and pulsotypes, harboured IMESp2907 in a new genetic environment, which was further investigated in one of the two isolates, SagTR7. IMESp2907 was circularizable in S. agalactiae, as described in S. pyogenes. The new IMESp2907 junctions were identified based on its site-specific integration; the att sites were almost identical to those in S. pyogenes. In strain SagTR7, erm(TR)-carrying IMESp2907 was embedded in an erm(TR)-less internal element related to ICE10750-RD.2/Tn1806, which, in turn, was embedded in an ICESde3396-like element. The resulting whole ICE, ICESagTR7 (∼129 kb), was integrated into the chromosome downstream of the rplL gene, and was excisable in circular form and transferable by conjugation. This is the first study exploring erm(TR)-carrying genetic elements in S. agalactiae. © The Author 2015. Published by

  4. Trauma Informed Guilt Reduction (TrIGR) Intervention

    Science.gov (United States)

    2016-10-01

    posttraumatic distress and a range of adverse outcomes, including posttraumatic stress disorder (PTSD), depression and suicidality , and alcohol...outcomes, including posttraumatic stress disorder (PTSD), depression and suicidality , and alcohol/substance use disorders. There is a pressing need for...RECIPIENT: Veterans Medical Research Foundation San Diego, CA 92161 REPORT DATE: October 2016 TYPE OF REPORT: Annual PREPARED FOR: U.S. Army

  5. Tallinna linna- ja gümnaasiumi trükikoda ehk 380 aastat trükikunsti Tallinnas / Aija Sakova-Merivee

    Index Scriptorium Estoniae

    Sakova-Merivee, Aija, 1980-

    2015-01-01

    Alanud aasta alguses esitlesid Tallinna Ülikooli Akadeemiline Raamatukogu ja Tallinna Linnaarhiiv ühist trükist „Tallinna linna- ja gümnaasiumi trükikoda (1634–1828). Näituse kataloog”. Autor annab ülevaate nii näitusest kui selle kataloogist

  6. Tanel Trööbil hea klapp välispartneritega / Tanel Trööp ; interv. Marika Tomberg

    Index Scriptorium Estoniae

    Trööp, Tanel, 1971-

    2004-01-01

    Reklaamifirma Jaa Disain juht Tanel Trööp oma senisest karjäärist, juhtimismeetoditest, tegevusest ja eesmärkidest ettevõtte eesotsas. Kommenteerivad Taavi Reigam ja Eno Juul. Lisa: Tanel Trööp

  7. Riso imotivado: relato de três casos

    Directory of Open Access Journals (Sweden)

    Paulo Henrique F. Bertolucci

    1985-03-01

    Full Text Available Os autores fazem uma revisão das causas e fisiopatologia do riso imoti-vado e de sua classificação. São apresentados três casos de riso imotivado. Um em que o distúrbio está associado a alterações motoras e sensitivas ocorrendo em crises diferentes entre si e sempre seguidas de recuperação integral, no qual foi feito o diagnóstico de esclerose múltipla. No segundo caso o riso estava associado a déficit motor e livedo reticular, tendo sido feito o diagnóstico de vasculite. No terceiro caso havia associação com paresia facial central à direita, em paciente diabético e hipertenso, com evidência de comprometimento vascular em outras áreas, tendo sido feito o diagnóstico de doença cerebrovascular.

  8. Colombia: ¿una sociedad trágica?

    Directory of Open Access Journals (Sweden)

    Sandro Romero Rey

    2014-10-01

    Full Text Available El texto forma parte de una reflexión sobre las especificidades de las ideas de tragedia y lo trágico dentro del conflicto colombiano contemporáneo. A partir de los elementos esenciales del teatro griego asociados al género y a la dimensión de la fatalidad, el presente texto establece relaciones entre los referentes de los mitos antiguos y sus equivalencias en una sociedad específica de América Latina. Los personajes de la tragedia griega se convierten en una suerte de metáforas vivas que continúan interrogando al hombre más allá de los límites de su tiempo.

  9. Træ og Stål i Havvand

    DEFF Research Database (Denmark)

    Jensen, Jens Kristian Jehrbo

    Dette kompendium behandler træ- og stålkonstruktioners holdbarhed i marint miljø. Materialerne bliver udsat for kraftige påvirkninget· i form af bølgeslag, strø ninger, slid, nedbrydning og korrosion. Relevante mekanismer og beskyttelsessysleaer behandles . J~ftet tænkes anvendt ved undervisninge...... , spec ielt på anlægssektorens 6. semester, men andre interesserede er velkomne til at benytte det. Renskrivningen er foretaget af Tove Jensen, og Ingrid Christensen og Poul Skørbæk Sørensen har udført tegnearbejdet. Tak for veludført arbejde! Jens Kr . Jehcbo Jensen...

  10. Ground-state correlations in 40Ca and 48Ca

    Science.gov (United States)

    Takayanagi, K.; Lipparini, E.

    1992-01-01

    Second-order perturbation theory with a G-matrix is adopted to examine from a unified point of view the effects of two-particle-two-hole correlations on the matter and momentum distributions and the occupation numbers in 40Ca and 48Ca. Polarization effects induced by the neutron excess in 48Ca are investigated in detail.

  11. Fabrication of PVDF-TrFE based bilayered PbTiO{sub 3}/PVDF-TrFE films capacitor

    Energy Technology Data Exchange (ETDEWEB)

    Nurbaya, Z., E-mail: nurbayazainal@gmail.com [NANO-ElecTronic Centre (NET), Faculty of Electrical Engineering, Universiti Teknologi MARA, 40450 Shah Alam, Selangor (Malaysia); Razak School of Engineering and Advanced Technology, Universiti Teknologi Malaysia, 54100 Kuala Lumpur (Malaysia); Wahid, M. H.; Rozana, M. D. [Faculty of Applied Sciences, Department of Polymer, Universiti Teknologi MARA, 40450 Shah Alam, Selangor (Malaysia); Annuar, I. [UiTM Sarawak Kampus Kota Samarahan, Jalan Meranek, 94300 Sarawak (Malaysia); Alrokayan, S. A. H.; Khan, H. A. [Department of Biochemistry, College of Science, Bldg. 5, King Saud University (KSU) P.O: 2454 Riyadh 11451 (Saudi Arabia); Rusop, M., E-mail: nanouitm@gmail.com [NANO-ElecTronic Centre (NET), Faculty of Electrical Engineering, Universiti Teknologi MARA, 40450 Shah Alam, Selangor (Malaysia); NANO-Sci Tech Centre (NST), Institute of Science, Universiti Teknologi MARA, 40450 Shah Alam, Selangor (Malaysia)

    2016-07-06

    Development of high performance capacitor is reaching towards new generation where the ferroelectric materials take places as the active dielectric layer. The motivation of this study is to produce high capacitance device with long life cycle. This was configured by preparing bilayered films where lead titanate as an active dielectric layer and stacked with the top dielectric layer, poly(vinyledenefluoride-trifluoroethylene). Both of them are being referred that have one in common which is ferroelectric behavior. Therefore the combination of ceramic and polymer ferroelectric material could perform optimum dielectric characteristic for capacitor applications. The fabrication was done by simple sol-gel spin coating method that being varied at spinning speed property for polymer layers, whereas maintaining the ceramic layer. The characterization of PVDF-TrFE/PbTiO3 was performed according to metal-insulator-metal stacked capacitor measurement which includes structural, dielectric, and ferroelectric measurement.

  12. Administrateur de la trésorerie (h/f) | CRDI - Centre de recherches ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    Relevant du comptable trésorerie, l'administrateur de la trésorerie prépare les lots de paiements pour tous les modes de paiement (télévirements internationaux, transferts électroniques de fonds et chambres de compensation automatisées, chèques). Il établit le plan de trésorerie quotidien pour les opérations du siège et ...

  13. RXR agonist modulates TR: corepressor dissociation upon 9-cis retinoic acid treatment.

    Science.gov (United States)

    Fattori, Juliana; Campos, Jéssica L O; Doratioto, Tábata R; Assis, Lucas M; Vitorino, Mariela T; Polikarpov, Igor; Xavier-Neto, José; Figueira, Ana Carolina M

    2015-02-01

    Transcriptional regulation controlled by thyroid hormone receptor (TR) drives events such as development, differentiation, and metabolism. TRs may act either as homodimers or as heterodimers with retinoid X receptor (RXR). Thyroid hormone T3 preferentially binds TR-RXR heterodimers, which activate transcription through coactivator recruitment. However, it is unclear whether TR-RXR heterodimers may also be responsive to the canonical RXR agonist 9-cis retinoic acid (9C) in the context of physiological gene regulation. New structural studies suggest that 9C promotes the displacement of bound coactivators from the heterodimer, modifying TR-RXR activity. To shed light on the molecular mechanisms that control TR-RXR function, we used biophysical approaches to characterize coregulator recruitment to TR-TR or to TR-RXR in the presence of T3 and/or 9C as well as cell-based assays to establish the functional significance of biophysical findings. Using cell-based and fluorescence assays with mutant and wild-type TR, we show that 9C does indeed have a function in the TR-RXR heterodimer context, in which it induces the release of corepressors. Furthermore, we show that 9C does not promote detectable conformational changes in the structure of the TR-RXR heterodimer and does not affect coactivator recruitment. Finally, our data support the view that DNA binding domain and Hinge regions are important to set up NR-coactivator binding interfaces. In summary, we showed that the RXR agonist 9C can regulate TR function through its modulation of corepressor dissociation.

  14. Disfunção miocárdica e alterações no trânsito de cálcio intracelular em ratos obesos

    Directory of Open Access Journals (Sweden)

    Ana Paula Lima-Leopoldo

    2011-09-01

    Full Text Available FUNDAMENTO: Vários mecanismos têm sido propostos contribuir para a disfunção cardíaca em modelos de obesidade, tais como alterações nas proteínas do trânsito de cálcio (Ca+2 e nos receptores beta-adrenérgicos. Todavia, o papel desses fatores no desenvolvimento da disfunção miocárdica induzida pela obesidade ainda não está claro. OBJETIVO: Este estudo pretende investigar se a obesidade induzida por um ciclo de dieta hipercalóricas resulta em disfunção cardíaca. Além disso, foi avaliado se essa alteração funcional em ratos obesos está relacionada com o prejuízo do trânsito de Ca+2 e do sistema beta-adrenérgico. MÉTODOS: Ratos Wistar machos, 30 dias de idade, foram alimentados com ração padrão (C e um ciclo de cinco dietas hipercalóricas (Ob por 15 semanas. A obesidade foi definida pelo aumento da porcentagem de gordura corporal dos ratos. A função cardíaca foi avaliada mediante análise isolada do músculo papilar do ventrículo esquerdo em condições basais e após manobras inotrópicas e lusitrópicas. RESULTADOS: Em comparação com o grupo controle, os ratos obesos apresentaram aumento da gordura corporal e intolerância a glicose. Os músculos dos ratos obesos desenvolveram valores basais semelhantes; entretanto, as respostas miocárdicas ao potencial pós-pausa e aumento de Ca+2 extracelular foram comprometidas. Não houve alterações na função cardíaca entre os grupos após a estimulação beta-adrenérgica. CONCLUSÃO: A obesidade promove disfunção cardíaca relacionada com alterações no trânsito de Ca+2 intracelular. Esse prejuízo funcional é provavelmente ocasionado pela redução da atividade da bomba de Ca+2 do retículo sarcoplasmático (SERCA2a via Ca+2 calmodulina-quinase.

  15. Divergence of Ca(2+) selectivity and equilibrium Ca(2+) blockade in a Ca(2+) release-activated Ca(2+) channel.

    Science.gov (United States)

    Yamashita, Megumi; Prakriya, Murali

    2014-03-01

    Prevailing models postulate that high Ca(2+) selectivity of Ca(2+) release-activated Ca(2+) (CRAC) channels arises from tight Ca(2+) binding to a high affinity site within the pore, thereby blocking monovalent ion flux. Here, we examined the contribution of high affinity Ca(2+) binding for Ca(2+) selectivity in recombinant Orai3 channels, which function as highly Ca(2+)-selective channels when gated by the endoplasmic reticulum Ca(2+) sensor STIM1 or as poorly Ca(2+)-selective channels when activated by the small molecule 2-aminoethoxydiphenyl borate (2-APB). Extracellular Ca(2+) blocked Na(+) currents in both gating modes with a similar inhibition constant (Ki; ~25 µM). Thus, equilibrium binding as set by the Ki of Ca(2+) blockade cannot explain the differing Ca(2+) selectivity of the two gating modes. Unlike STIM1-gated channels, Ca(2+) blockade in 2-APB-gated channels depended on the extracellular Na(+) concentration and exhibited an anomalously steep voltage dependence, consistent with enhanced Na(+) pore occupancy. Moreover, the second-order rate constants of Ca(2+) blockade were eightfold faster in 2-APB-gated channels than in STIM1-gated channels. A four-barrier, three-binding site Eyring model indicated that lowering the entry and exit energy barriers for Ca(2+) and Na(+) to simulate the faster rate constants of 2-APB-gated channels qualitatively reproduces their low Ca(2+) selectivity, suggesting that ion entry and exit rates strongly affect Ca(2+) selectivity. Noise analysis indicated that the unitary Na(+) conductance of 2-APB-gated channels is fourfold larger than that of STIM1-gated channels, but both modes of gating show a high open probability (Po; ~0.7). The increase in current noise during channel activation was consistent with stepwise recruitment of closed channels to a high Po state in both cases, suggesting that the underlying gating mechanisms are operationally similar in the two gating modes. These results suggest that both high affinity Ca

  16. Nietzsche e o renascimento do trágico

    Directory of Open Access Journals (Sweden)

    Roberto Machado

    2005-12-01

    Full Text Available O objetivo deste artigo é expor como a proposta de renascimento do trágico existente em O nascimento da tragédia insere o primeiro livro de Nietzsche no projeto cultural iniciado por Winckelmann, Goethe e Schiller, na segunda metade do século XVIII, que privilegia a arte grega como modelo da arte alemã. Destacarei, para isso, tanto as continuidades entre os dois momentos quanto as descontinuidades que Nietzsche introduz nesse projeto clássico da intelectualidade alemã ao pensar a cultura grega a partir da filosofia de Schopenhauer e da música de Wagner.The purpose of this article is to discuss Nietzsche's theory on the rebirth of the tragic presented in his early writings as part of the German cultural project introduced by Winckelmann, Goethe and Schiller in the second half of the 18th century. Departing from the idea that this project established Greece as a model to be followed by Germany, I will point out Nietzsche's supporting views to it as well as his innovative idea of bringing together the philosophy of Schopenhauer and the music of Wagner to think the Greek culture.

  17. TR-EDB: Test Reactor Embrittlement Data Base, Version 1

    Energy Technology Data Exchange (ETDEWEB)

    Stallmann, F.W.; Wang, J.A.; Kam, F.B.K. [Oak Ridge National Lab., TN (United States)

    1994-01-01

    The Test Reactor Embrittlement Data Base (TR-EDB) is a collection of results from irradiation in materials test reactors. It complements the Power Reactor Embrittlement Data Base (PR-EDB), whose data are restricted to the results from the analysis of surveillance capsules in commercial power reactors. The rationale behind their restriction was the assumption that the results of test reactor experiments may not be applicable to power reactors and could, therefore, be challenged if such data were included. For this very reason the embrittlement predictions in the Reg. Guide 1.99, Rev. 2, were based exclusively on power reactor data. However, test reactor experiments are able to cover a much wider range of materials and irradiation conditions that are needed to explore more fully a variety of models for the prediction of irradiation embrittlement. These data are also needed for the study of effects of annealing for life extension of reactor pressure vessels that are difficult to obtain from surveillance capsule results.

  18. Georges et les trésors du cosmos

    CERN Document Server

    Hawking, Lucy; Parsons, Gary

    2011-01-01

    Les voisins excentriques de Georges, Annie et Eric, ont déménagé en Floride à l'agence Spatiale Globale. Là-bas, Eric s'occupe de son nouveau robot, Homer, qui doit détécter les signes de vie sur Mars. Mais bientôt, Georges reçoit un e-mail : Annie lui demande de la rejoindre au plus vite pour une " mission cosmique " secrète. La jeune fille est persuadée qu'il se passe de drôles de choses sur Mars, car Homer vient de recevoir un message extraterrestre ! Georges et Anne (avec l'aide d'Emmett, un petit garçon bizarre) décident de réparer Cosmos, leur super-ordinateur, et d'aller voir par eux-mêmes... Emmaillotés dans des combinaisons spéciales, ils se lancent alors dans une fabuleuse chasse aux trésors sur Mars, sur les lunes de Saturne, puis sur Titan, avant de se perdre en orbite autour d'Alpha Centauri B... Mais avant de pouvoir élucider le mystère du message extraterrestre, Georges et Annie devront se livrer à une bataille spatiale acharnée qui mettra leur vie en danger, ainsi que ce...

  19. El tráfico de órganos en Brasil: Civilconstitucional enderece contra la Mercantilización persona

    Directory of Open Access Journals (Sweden)

    Mara Conceição Vieira de Oliveira

    2016-06-01

    Full Text Available Pese haya regulación en el sistema jurídico brasileño, el Tráfico de órganos es problema serio, y medidas de prevención son poco discutidas y/o presentadas. La atrocidad del cri- men se vuelve poco creíble ante la sociedad, en la cual muchos entienden el tráfico de ór- ganos sólo como más una leyenda urbana. Este estudio cuestiona: ¿Cuál la efectividad de la regulación de la dignidad de la persona humana, cuando el cuerpo asume valor de “cosa”?, es decir, ¿Cuánto cuesta una persona o parte suya cuando transformada en producto en una sociedad de mercado? ¿Cuáles los límites jurídicos y morales son violados cuando un órgano está puesto a la venta? De esta manera, se objetiva, aquí, describir el principio de la dignidad de la persona humana y su efectividad en relación a este crimen, señalando el incumplimiento del Código Civil Brasileiro. Para eso, el procedimiento metodológico ele- gido se orientará por la investigación teórico bibliográfica, al realizar lecturas legislativas y doctrinarias; lectura crítica filosófica con Michael Sandel sobre la moralidad del mercado; y recopilación de datos sobre la base de la CPI del Tráfico de órganos – “CPIORGAO”. Los resultados obtenidos, hasta el momento actual, presentan recopilación teórico bibliográfi- ca; aún no se realizó análisis aplicada a los casos específicos citados por la comisión, a pesar de que puedan venir a ser estudiados en otras etapas del desarrollo de la investigación. La temática de este artículo, al relacionar la problemática del tráfico de órganos, la dignidad de la persona humana y la mercantilización del cuerpo, contribuye para el tema del evento: - Participación, Democracia y Ciudadanía en la perspectiva del Derecho Iberoameri- cano -, una vez que nuestro grupo de investigación también entiende la cuestión en este escenario de creciente y complejo proceso de globalización.

  20. The Significance of the Trömner Sign in Cervical Spondylotic Myelopathy Patient.

    Science.gov (United States)

    Chaiyamongkol, Weera; Laohawiriyakamol, Teeranan; Tangtrakulwanich, Boonsin; Tanutit, Pramot; Bintachitt, Piyawat; Siribumrungwong, Koopong

    2017-11-01

    This study is a diagnostic analysis. To investigate the diagnostic accuracy of Trömner sign in cervical spondylotic myelopathy (CSM), and how its presence correlates with the severity of myelopathy. A clinical presentation of myelopathy corresponding with image findings is a current standard to diagnose CSM. Trömner sign is an alternative of well-known Hoffmann sign to detect CSM. Little is known about its diagnostic accuracy and how its presence correlates with the severity of CSM. Consecutive patients with clinical diagnosis of CSM and other cervical spondylosis-related problems were enrolled in either CSM group, cervical spondylotic radiculopathy group, or axial pain group. Normal volunteers and patients without spine-related issues were used as a control. All participants were examined for the presence of myelopathic signs. Magnetic resonance imaging studies of all participants were reviewed by a radiologist. There were 85 participants included in the study. Diagnostic sensitivity was 76%, 94%, 76%, and 36% for Hoffmann sign, Trömner sign, inverted radial reflex, and Babinski sign, respectively. Trömner sign had relatively high sensitivity (95%) despite of mild degree of myelopathy. Negative predictive value was 60%, 85%, 59%, and 38% for Hoffmann sign, Trömner sign, inverted radial reflex, and Babinski sign, respectively. There were 63%-71% of patients in either axial pain group or cervical spondylotic radiculopathy group had positive Trömner sign. Most of CSM patients with cord signal changed had positive myelopathic sign. Regarding CSM patient without cord signal change, most of tests were negative except Trömner sign. High sensitivity (94%) and relatively high negative predictive value (85%) for Trömner sign indicate the usefulness of Trömner sign in ruling out CSM. High incidence of positive Trömner sign in presymptomatic cervical cord compression patients suggests Trömner sign could have a useful role in early detection of presymptomatic

  1. Gas Metal Arc Welding Using Novel CaO-Added Mg Alloy Filler Wire

    OpenAIRE

    Minjung Kang; Youngnam Ahn; Cheolhee Kim

    2016-01-01

    Novel “ECO Mg” alloys, i.e., CaO-added Mg alloys, which exhibit oxidation resistance during melting and casting processes, even without the use of beryllium or toxic protection gases such as SF6, have recently been introduced. Research on ECO Mg alloys is still continuing, and their application as welding filler metals was investigated in this study. Mechanical and metallurgical aspects of the weldments were analysed after welding, and welding behaviours such as fume generation and droplet tr...

  2. Rompendo com a criminalidade: saída de jovens do tráfico de drogas em favelas na cidade do Rio de Janeiro Stopping with the criminality: a way out of youths involved in traffic of drugs in slums in the city of Rio de Janeiro

    Directory of Open Access Journals (Sweden)

    Zilah Vieira Meirelles

    2009-12-01

    Full Text Available O presente artigo aborda a vida de jovens egressos do tráfico de drogas nas favelas da cidade do Rio de Janeiro. Seu principal objetivo consiste em analisar as circunstâncias e condições específicas que impulsionam os jovens a abandonarem essa atividade, ilícita, de trabalho. O método utilizado para a coleta de dados foi a história de vida tópica que permitiu conhecer o percurso de entrada e saída de trinta jovens do tráfico, moradores de sete favelas. Os dados revelam que os jovens, ao entrarem para o tráfico, apresentam uma expectativa de encantamento, poder e dinheiro. Com o tempo, essa perspectiva vai desaparecendo devido às situações de traição, punição e medo. O processo de saída ocorre quando o jovem começa a questionar este estilo de trabalho e procura visualizar outras possibilidades de vida, mais condizentes com as suas aspirações. Concluiu-se que o fato de os jovens estarem entrando precocemente no crime organizado leva-os a um desgaste físico e emocional, visto que a venda de drogas passou a ser um coadjuvante frente aos constantes episódios de conflitos armados e tráfico de armas.This article deals with the lives of young egress of drug traffic in the slums of the city of Rio de Janeiro. Its main objective is to examine the circumstances and the specific conditions that drive young people to abandon this illegal work activity. The method used to collect data was the topical life story in order to find out how 30 youths, from seven slums, had got involved until their departure from the "movement". Data shows that youths, entering trafficking, had an expectation of empowerment. With time, this attitude will wane, as they realize they are living with situations of betrayal, punishment and fear. The process for getting out occurs when the youth begins to question this type of work and sees other ways of life, more consistent with their aspirations. The facts conclude that when getting an early involvement in

  3. Optimization and Verification of the TR-MAC Protocol for Wireless Sensor Networks

    NARCIS (Netherlands)

    Morshed, S.; Heijenk, Geert

    2015-01-01

    Energy-efficiency is an important requirement in the design of communication protocols for wireless sensor networks (WSN). TR-MAC is an energy-efficient medium access control (MAC) layer protocol for low power WSN that exploits transmitted-reference (TR) modulation in the physical layer. The

  4. Samlingsdetaljers indflydelse på fugtniveauet i træfacader

    DEFF Research Database (Denmark)

    Nicolajsen, Asta; Hansen, Morten Hjorslev

    Fugtophobningen i en række træfacadeelementer med forskellige konstruktionsopbygninger med eller uden fejl i samlingerne er bestemt under laboratorieforhold. Rapporten dokumenterer, at fejl i samlinger mellem træfacadeelementer medfører højere fugtindhold i konstruktionstræet, end når samlingerne...

  5. Initial code exchange for asynchronous TR-UWB ad hoc networks

    NARCIS (Netherlands)

    Djapic, R.; Shi, N.; Leus, G.

    2007-01-01

    In this paper we present an initial code exchange scheme for a transmit-reference ultra wide-band (TR-UWB) system. We exploit the facts that a TR-UWB transceiver scheme allows for simultaneous transmission and reception as well as detection and separation of distinct users with the same code even if

  6. The Orphan Nuclear Receptor TR4 Is a Vitamin A-activated Nuclear Receptor

    Energy Technology Data Exchange (ETDEWEB)

    Zhou, X. Edward; Suino-Powell, Kelly M.; Xu, Yong; Chan, Cee-Wah; Tanabe, Osamu; Kruse, Schoen W.; Reynolds, Ross; Engel, James Douglas; Xu, H. Eric (Michigan-Med); (Van Andel)

    2015-11-30

    Testicular receptors 2 and 4 (TR2/4) constitute a subgroup of orphan nuclear receptors that play important roles in spermatogenesis, lipid and lipoprotein regulation, and the development of the central nervous system. Currently, little is known about the structural features and the ligand regulation of these receptors. Here we report the crystal structure of the ligand-free TR4 ligand binding domain, which reveals an autorepressed conformation. The ligand binding pocket of TR4 is filled by the C-terminal half of helix 10, and the cofactor binding site is occupied by the AF-2 helix, thus preventing ligand-independent activation of the receptor. However, TR4 exhibits constitutive transcriptional activity on multiple promoters, which can be further potentiated by nuclear receptor coactivators. Mutations designed to disrupt cofactor binding, dimerization, or ligand binding substantially reduce the transcriptional activity of this receptor. Importantly, both retinol and retinoic acid are able to promote TR4 to recruit coactivators and to activate a TR4-regulated reporter. These findings demonstrate that TR4 is a ligand-regulated nuclear receptor and suggest that retinoids might have a much wider regulatory role via activation of orphan receptors such as TR4.

  7. The anti-inflammatory effect of TR6 on LPS-induced mastitis in mice.

    Science.gov (United States)

    Hu, Xiaoyu; Fu, Yunhe; Tian, Yuan; Zhang, Zecai; Zhang, Wenlong; Gao, Xuejiao; Lu, Xiaojie; Cao, Yongguo; Zhang, Naisheng

    2016-01-01

    [TRIAP]-derived decoy peptides have anti-inflammatory properties. In this study, we synthesized a TRIAP-derived decoy peptide (TR6) containing, the N-terminal portion of the third helical region of the [TIRAP] TIR domain (sequence "N"-RQIKIWFQNRRMKWK and -KPGFLRDPWCKYQML-"C"). We evaluated the effects of TR6 on lipopolysaccharide-induced mastitis in mice. In vivo, the mastitis model was induced by LPS administration for 24h, and TR6 treatment was initiated 1h before or after induction of LPS. In vitro, primary mouse mammary epithelial cells and neutrophils were used to investigate the effects of TR6 on LPS-induced inflammatory responses. The results showed that TR6 significantly inhibited mammary gland hisopathologic changes, MPO activity, and LPS-induced production of TNF-α, IL-1β and IL-6. In vitro, TR6 significantly inhibited LPS-induced TNF-α and IL-6 production and phosphorylation of NF-κB and MAPKs. In conclusion, this study demonstrated that the anti-inflammatory effect of TR6 against LPS-induced mastitis may be due to its ability to inhibit TLR4-mediated NF-κB and MAPK signaling pathways. TR6 may be a promising therapeutic reagent for mastitis treatment. Copyright © 2015. Published by Elsevier B.V.

  8. Xylan oligosaccharides and cellobiohydrolase I (TrCeI7A) interaction and effect on activity

    DEFF Research Database (Denmark)

    Baumann, Martin Johannes; Borch, Kim; Westh, Peter

    2011-01-01

    and an enzyme variant without the cellulose-binding domain (CBM). Results We studied the binding of XOSs to TrCel7A by isothermal titration calorimetry. We found that XOSs bind to TrCel7A and that the affinity increases commensurate with XOS length. The CBM, on the other hand, did not affect the affinity...

  9. /sup 40/Ca-/sup 48/Ca isotope shift

    Energy Technology Data Exchange (ETDEWEB)

    Khodel, V.A.; Platonov, A.P.; Saperstein, E.E. (Gosudarstvennyj Komitet po Ispol' zovaniyu Atomnoj Ehnergii SSSR, Moscow. Inst. Atomnoj Ehnergii)

    1982-07-01

    Contributions of the virtual low-lying collective excitations to the nuclear density are calculated within the framework of the theory of self-consistent finite Fermi systems. It is shown that this effect is responsible for the /sup 40/Ca-/sup 48/Ca isotope shift.

  10. TR146 cells grown on filters as a model of human buccal epithelium

    DEFF Research Database (Denmark)

    Nielsen, H M; Rassing, M R; Nielsen, Hanne Mørck

    2000-01-01

    The objective of the present study was to evaluate the TR146 cell culture model as an in vitro model of human buccal epithelium. For this purpose, the permeability of water, mannitol and testosterone across the TR146 cell culture model was compared to the permeability across human, monkey...... (logD(oct; 7.4)) and capacity factor (k') and to their polar water accessible surface area (PWASA). For water, mannitol, testosterone and some of the beta-adrenoceptor antagonists, the permeability enhancement across the TR146 cell culture model in the presence of sodium glycocholate (GC......) was determined. The mannitol and testosterone permeability across the TR146 cell culture model could be related to the permeability across porcine and human buccal mucosa. The permeability of the beta-adrenoceptor antagonists across the TR146 cell culture model varied between 2.2 x 10(-6) cm/s (atenolol) and 165...

  11. TR146 cells grown on filters as a model of human buccal epithelium

    DEFF Research Database (Denmark)

    Mørck Nielsen, H; Rømer Rassing, M; Nielsen, Hanne Mørck

    2000-01-01

    The objective of the present study was to characterise the TR146 cell culture model as an in vitro model of human buccal mucosa with respect to the enzyme activity in the tissues. For this purpose, the contents of aminopeptidase, carboxypeptidase and esterase in homogenate supernatants of the TR146...... cell culture model, and human and porcine buccal epithelium were compared. The esterase activity in the intact cell culture model and in the porcine buccal mucosa was compared. Further, the TR146 cell culture model was used to study the permeability rate and metabolism of leu-enkephalin. The activity...... of the three enzymes in the TR146 homogenate supernatants was in the same range as the activity in homogenate supernatants of human buccal epithelium. In the TR146 cell culture model, the activity of aminopeptidase (13.70+/-2.10 nmol/min per mg protein) was approx. four times the activity of carboxypeptidase...

  12. Mitochondrial Ca2+ homeostasis during Ca2+ influx and Ca2+ release in gastric myocytes from Bufo marinus

    Science.gov (United States)

    Drummond, Robert M; Mix, T Christian H; Tuft, Richard A; Walsh, John V; Fay, Fredric S

    2000-01-01

    The Ca2+-sensitive fluorescent indicator rhod-2 was used to monitor mitochondrial Ca2+ concentration ([Ca2+]m) in gastric smooth muscle cells from Bufo marinus. In some studies, fura-2 was used in combination with rhod-2, allowing simultaneous measurement of cytoplasmic Ca2+ concentration ([Ca2+]i) and [Ca2+]m, respectively. During a short train of depolarizations, which causes Ca2+ influx from the extracellular medium, there was an increase in both [Ca2+]i and [Ca2+]m. The half-time (t½) to peak for the increase in [Ca2+]m was considerably longer than the t½ to peak for the increase in [Ca2+]i. [Ca2+]m remained elevated for tens of seconds after [Ca2+]i had returned to its resting value. Stimulation with caffeine, which causes release of Ca2+ from the sarcoplasmic reticulum (SR), also produced increases in both [Ca2+]i and [Ca2+]m. The values of t½ to peak for the increase in [Ca2+] in both cytoplasm and mitochondria were similar; however, [Ca2+]i returned to baseline values much faster than [Ca2+]m. Using a wide-field digital imaging microscope, changes in [Ca2+]m were monitored within individual mitochondria in situ, during stimulation of Ca2+ influx or Ca2+ release from the SR. Mitochondrial Ca2+ uptake during depolarizing stimulation caused depolarization of the mitochondrial membrane potential. The mitochondrial membrane potential recovered considerably faster than the recovery of [Ca2+]m. This study shows that Ca2+ influx from the extracellular medium and Ca2+ release from the SR are capable of increasing [Ca2+]m in smooth muscle cells. The efflux of Ca2+ from the mitochondria is a slow process and appears to be dependent upon the amount of Ca2+ in the SR. PMID:10713963

  13. Distance Mapping in Proteins Using Fluorescence Spectroscopy: The Tryptophan-Induced Quenching (TrIQ) Method

    Science.gov (United States)

    Mansoor, Steven E.; DeWitt, Mark A.; Farrens, David L.

    2014-01-01

    Studying the interplay between protein structure and function remains a daunting task. Especially lacking are methods for measuring structural changes in real time. Here we report our most recent improvements to a method that can be used to address such questions. This method, which we now call Tryptophan induced quenching (TrIQ), provides a straightforward, sensitive and inexpensive way to address questions of conformational dynamics and short-range protein interactions. Importantly, TrIQ only occurs over relatively short distances (~5 to 15 Å), making it complementary to traditional fluorescence resonance energy transfer (FRET) methods that occur over distances too large for precise studies of protein structure. As implied in the name, TrIQ measures the efficient quenching induced in some fluorophores by tryptophan (Trp). We present here our analysis of the TrIQ effect for five different fluorophores that span a range of sizes and spectral properties. Each probe was attached to four different cysteine residues on T4 lysozyme and the extent of TrIQ caused by a nearby Trp was measured. Our results show that for smaller probes, TrIQ is distance dependent. Moreover, we also demonstrate how TrIQ data can be analyzed to determine the fraction of fluorophores involved in a static, non-fluorescent complex with Trp. Based on this analysis, our study shows that each fluorophore has a different TrIQ profile, or "sphere of quenching", which correlates with its size, rotational flexibility, and the length of attachment linker. This TrIQ-based "sphere of quenching" is unique to every Trp-probe pair and reflects the distance within which one can expect to see the TrIQ effect. It provides a straightforward, readily accessible approach for mapping distances within proteins and monitoring conformational changes using fluorescence spectroscopy. PMID:20886836

  14. Thin filament Ca2+ binding properties and regulatory unit interactions alter kinetics of tension development and relaxation in rabbit skeletal muscle.

    Science.gov (United States)

    Kreutziger, Kareen L; Piroddi, Nicoletta; Scellini, Beatrice; Tesi, Chiara; Poggesi, Corrado; Regnier, Michael

    2008-08-01

    The influence of Ca(2+) binding properties of individual troponin versus cooperative regulatory unit interactions along thin filaments on the rate tension develops and declines was examined in demembranated rabbit psoas fibres and isolated myofibrils. Native skeletal troponin C (sTnC) was replaced with sTnC mutants having altered Ca(2+) dissociation rates (k(off)) or with mixtures of sTnC and D28A, D64A sTnC, that does not bind Ca(2+) at sites I and II (xxsTnC), to reduce near-neighbour regulatory unit (RU) interactions. At saturating Ca(2+), the rate of tension redevelopment (k(TR)) was not altered for fibres containing sTnC mutants with decreased k(off) or mixtures of sTnC:xxsTnC. We examined the influence of k(off) on maximal activation and relaxation in myofibrils because they allow rapid and large changes in [Ca(2+)]. In myofibrils with M80Q sTnC(F27W) (decreased k(off)), maximal tension, activation rate (k(ACT)), k(TR) and rates of relaxation were not altered. With I60Q sTnC(F27W) (increased k(off)), maximal tension, k(ACT) and k(TR) decreased, with no change in relaxation rates. Surprisingly, the duration of the slow phase of relaxation increased or decreased with decreased or increased k(off), respectively. For all sTnC reconstitution conditions, Ca(2+) dependence of k(TR) in fibres showed Ca(2+) sensitivity of k(TR) (pCa(50)) shifted parallel to tension and low-Ca(2+) k(TR) was elevated. Together the data suggest the Ca(2+)-dependent rate of tension development and the duration (but not rate) of relaxation can be greatly influenced by k(off) of sTnC. This influence of sTnC binding kinetics occurs primarily within individual RUs, with only minor contributions of RU interactions at low Ca(2+).

  15. Adenovirus platform enhances transduction efficiency of human mesenchymal stem cells: An opportunity for cellular carriers of targeted TRAIL-based TR3 biologics in ovarian cancer.

    Directory of Open Access Journals (Sweden)

    Lindsay M Kuroki

    Full Text Available Clinical application of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL-based cancer therapeutics has not reached optimal potencies in part due to inadequate drug stability and inefficiencies in cancer-selective drug delivery. As such, innovative strategies regarding drug design and delivery are of utmost importance to achieve improved treatment results. With our current study, we aimed at exploring the groundwork for a two-stage targeting concept, which is based on the intrinsic tumor homing capacity of mesenchymal stem cells (MSCs as cellular drug factories for the in situ production of our newly designed and biomarker-targeted TRAIL-based TR3 therapeutics. Since MSCs are primary cells, capable in vitro of only a limited number of cell divisions, identification of suitable strategies for their efficient genetic manipulation is of critical importance. We chose adenoviral (Ad vectors as a transduction vehicle due to its ability to infect dividing and non-dividing cells and because of their limited restrictions regarding the packaging capacity of their genetic payload. In order to enhance the transduction efficacy of MSCs using Ad5 wild-type-based vectors, we tested a variety of fiber knob modifications on a panel of patient-derived MSC lines established from adipose tissue. We identified Ad5pK7, an Ad5 vector containing a polylysine fiber knob modification, exhibiting the highest transduction rates across a panel of 16 patient-derived MSC lines. We further demonstrated that MSCs could be efficiently transduced with an Ad5pK7 vector containing membrane-anchored and secreted TR3 expression units, including the MUC16 (CA125-targeted variant Meso64-TR3. In both in vitro and in vivo experiments, MSC-derived Meso64-TR3 was far more potent on MUC16-expressing ovarian cancer compared to its non-targeted TR3 counterpart. Our findings thus provide the foundation to initiate further preclinical investigations on MSC-mediated treatment

  16. In-situ hybridization based quantification of hTR: a possible biomarker in malignant melanoma

    DEFF Research Database (Denmark)

    Vagner, Josephine; Steiniche, Torben; Stougaard, Magnus

    2015-01-01

    Aims Telomerase is reactivated in most cancers and there is accumulating evidence for it being a driver event in malignant melanoma (MM). Thus, our aim was to evaluate if in situ hybridisation (ISH)-based quantification of the telomerase RNA (hTR) could be used to distinguish MM from nevi...... thickness suggesting that hTR might be a valuable biomarker in MM. Furthermore, as ISH-based detection requires presence of both hTR and the reverse transcriptase (hTERT) it might be an indicator of active telomerase and thus have future relevance as a predictive biomarker for anti-telomerase treatment....

  17. Subchronic Exposure to Arsenic Represses the TH/TRβ1-CaMK IV Signaling Pathway in Mouse Cerebellum

    Directory of Open Access Journals (Sweden)

    Huai Guan

    2016-01-01

    Full Text Available We previously reported that arsenic (As impaired learning and memory by down-regulating calmodulin-dependent protein kinase IV (CaMK IV in mouse cerebellum. It has been documented that the thyroid hormone receptor (TR/retinoid X receptor (RXR heterodimer and thyroid hormone (TH may be involved in the regulation of CaMK IV. To investigate whether As affects the TR/RXR heterodimer and TH, we determined As concentration in serum and cerebellum, 3,5,3’-triiodothyronine (T3 and thyroxin (T4 levels in serum, and expression of CaMK IV, TR and RXR in cerebellum of mice exposed to As. Cognition function was examined by the step-down passive avoidance task and Morris water maze (MWM tests. Morphology of the cerebellum was observed by Hematoxylin-Eosin staining under light microscope. Our results showed that the concentrations of As in the serum and cerebellum of mice both increased with increasing As-exposure level. A significant positive correlation was found between the two processes. Adeficit in learning and memory was found in the exposed mice. Abnormal morphologic changes of Purkinje cells were observed in cerebellum of the exposed mice. Moreover, the cerebellar expressions of CaMK IV protein and the TRβ gene, and TRβ1 protein were significantly lower in As-exposed mice than those in controls. Subchronic exposure to As appears to increase its level in serum and cerebella of mice, impairing learning and memory and down-regulating expression of TRβ1 as well as down-stream CaMK IV. It is also suggested that the increased As may be responsible for down-regulation of TRβ1 and CaMK IV in cerebellum and that the down-regulated TRβ1 may be involved in As-induced impairment of learning and memory via inhibiting CaMK IV and its down-stream pathway.

  18. Subchronic Exposure to Arsenic Represses the TH/TRβ1-CaMK IV Signaling Pathway in Mouse Cerebellum.

    Science.gov (United States)

    Guan, Huai; Li, Shuangyue; Guo, Yanjie; Liu, Xiaofeng; Yang, Yi; Guo, Jinqiu; Li, Sheng; Zhang, Cong; Shang, Lixin; Piao, Fengyuan

    2016-01-26

    We previously reported that arsenic (As) impaired learning and memory by down-regulating calmodulin-dependent protein kinase IV (CaMK IV) in mouse cerebellum. It has been documented that the thyroid hormone receptor (TR)/retinoid X receptor (RXR) heterodimer and thyroid hormone (TH) may be involved in the regulation of CaMK IV. To investigate whether As affects the TR/RXR heterodimer and TH, we determined As concentration in serum and cerebellum, 3,5,3'-triiodothyronine (T3) and thyroxin (T4) levels in serum, and expression of CaMK IV, TR and RXR in cerebellum of mice exposed to As. Cognition function was examined by the step-down passive avoidance task and Morris water maze (MWM) tests. Morphology of the cerebellum was observed by Hematoxylin-Eosin staining under light microscope. Our results showed that the concentrations of As in the serum and cerebellum of mice both increased with increasing As-exposure level. A significant positive correlation was found between the two processes. Adeficit in learning and memory was found in the exposed mice. Abnormal morphologic changes of Purkinje cells were observed in cerebellum of the exposed mice. Moreover, the cerebellar expressions of CaMK IV protein and the TRβ gene, and TRβ1 protein were significantly lower in As-exposed mice than those in controls. Subchronic exposure to As appears to increase its level in serum and cerebella of mice, impairing learning and memory and down-regulating expression of TRβ1 as well as down-stream CaMK IV. It is also suggested that the increased As may be responsible for down-regulation of TRβ1 and CaMK IV in cerebellum and that the down-regulated TRβ1 may be involved in As-induced impairment of learning and memory via inhibiting CaMK IV and its down-stream pathway.

  19. CA125 in ovarian cancer

    DEFF Research Database (Denmark)

    Duffy, M J; Bonfrer, J M; Kulpa, J

    2005-01-01

    CA125 is currently the most widely used tumor marker for ovarian epithelial cancer. The aim of this article is to provide guidelines for the routine clinical use of CA125 in patients with ovarian cancer. Due to lack of sensitivity for stage I disease and lack of specificity, CA125 is of little...... value in the detection of early ovarian cancer. At present, therefore, CA125, either alone or in combination with other modalities, cannot be recommended for screening for ovarian cancer in asymptomatic women outside the context of a randomized controlled trial. Preoperative levels in postmenopausal...... women, however, may aid the differentiation of benign and malignant pelvic masses. Serial levels during chemotherapy for ovarian cancer are useful for assessing response to treatment. Although serial monitoring following initial chemotherapy can lead to the early detection of recurrent disease...

  20. Sonoma County, CA, 2013 Lidar

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Sonoma County Vegetation Mapping and LiDAR Consortium retained WSI to provide lidar and Orthophoto data and derived products in Sonoma County, CA. A classified LAS...

  1. Trémaux on species: a theory of allopatric speciation (and punctuated equilibrium) before Wagner.

    Science.gov (United States)

    Wilkins, John S; Nelson, Gareth J

    2008-01-01

    Pierre Trémaux's 1865 ideas on speciation have been unjustly derided following his acceptance by Marx and rejection by Engels, and almost nobody has read his ideas in a charitable light. Here we offer an interpretation based on translating the term sol as "habitat," in order to show that Trémaux proposed a theory of allopatric speciation before Wagner and a punctuated equilibrium theory before Gould and Eldredge, and we translate the relevant discussion from the French. We believe he may have influenced Darwin's revision to the third edition of the Origin on rates of evolution. We also suggest that Gould's dismissal of Trémaux is motivated by concern that others might think punctuated equilibrium theory was tainted by a connection with Trémaux.

  2. InGaP DHBT for High Efficiency L-band T/R Module Project

    Data.gov (United States)

    National Aeronautics and Space Administration — A fully monolithically integrated L-band T/R module using InGaP/GaAs-based HBTs (heterojunction bipolar transistors) for both the transmit and receive functions is...

  3. Draft Genome Sequence of Clostridium mangenotii TR, Isolated from the Fecal Material of a Timber Rattlesnake

    Science.gov (United States)

    Cochran, Philip A.; Dowd, Scot E.; Andersen, Kylie; Anderson, Nichole; Brennan, Rachel; Brook, Nicole; Callaway, Tracie; Diamante, Kimberly; Duberstine, Annie; Fitch, Karla; Freiheit, Heidi; Godlewski, Chantel; Gorman, Kelly; Haubrich, Mark; Hernandez, Mercedes; Hirtreiter, Amber; Ivanoski, Beth; Jaminet, Xochitl; Kirkpatrick, Travis; Kratowicz, Jennifer; Latus, Casey; Leable, Tiegen; Lingafelt, Nicole; Lowe, DeAnna; Lowrance, Holly; Malsack, Latiffa; Mazurkiewicz, Julie; Merlos, Persida; Messley, Jamie; Montemurro, Dawn; Nakitare, Samora; Nelson, Christine; Nye, Amber; Pazera, Valerie; Pierangeli, Gina; Rellora, Ashley; Reyes, Angelica; Roberts, Jennifer; Robins, Shadara; Robinson, Jeshannah; Schultz, Alissa; Seifert, Sara; Sigler, Elona; Spangler, Julie; Swift, Ebony; TenCate, Rebecca; Thurber, Jessica; Vallee, Kristin; Wamboldt, Jennifer; Whitten, Shannon; Woods, De’andrea; Wright, Amanda; Yankunas, Darin

    2014-01-01

    Here, we report the draft genome sequence of Clostridium mangenotii strain TR, which was isolated from the fecal material of a timber rattlesnake. This bacterium is nonpathogenic but contains 68 genes involved in virulence, disease, and defense. PMID:24407632

  4. Hüpertekst vs trükitekst / Kärt Matiisen

    Index Scriptorium Estoniae

    Matiisen, Kärt

    2003-01-01

    Eksperimendist Kose Gümnaasiumi 8. klasside õpilaste hulgas tõestamaks arvutiõppe eeliseid. Ilmnes, et 24 tundi hiljem mäletavad hüperteksti lugenud õpilased seda paremini kui klassikalist trükiteksti

  5. Graphene-P(VDF-TrFE) multilayer film for flexible applications.

    Science.gov (United States)

    Bae, Sang-Hoon; Kahya, Orhan; Sharma, Bhupendra K; Kwon, Junggou; Cho, Hyoung J; Özyilmaz, Barbaros; Ahn, Jong-Hyun

    2013-04-23

    A flexible, transparent acoustic actuator and nanogenerator based on graphene/P(VDF-TrFE)/graphene multilayer film is demonstrated. P(VDF-TrFE) is used as an effective doping layer for graphene and contributes significantly to decreasing the sheet resistance of graphene to 188 ohm/sq. The potentiality of graphene/P(VDF-TrFE)/graphene multilayer film is realized in fabricating transparent, flexible acoustic devices and nanogenerators to represent its functionality. The acoustic actuator shows good performance and sensitivity over a broad range of frequency. The output voltage and the current density of the nanogenerator are estimated to be ∼3 V and ∼0.37 μAcm(-2), respectively, upon the application of pressure. These values are comparable to those reported earlier for ZnO- and PZT-based nanogenerators. Finally, the possibility of rollable devices based on graphene/P(VDF-TrFE)/graphene structure is also demonstrated under a dynamic mechanical loading condition.

  6. Flexible T/R Modules for Large-Aperture, Space-Based SAR Project

    Data.gov (United States)

    National Aeronautics and Space Administration — SI2 Technologies, Inc (SI2) proposes to develop membrane compatible transmit/receive (T/R) modules for flexible, space-deployable synthetic aperture radar (SAR)...

  7. X Congreso Panamericano de Ingeniería de Tránsito y Transporte

    OpenAIRE

    Luis Antonio Lindau; Orlando Strambi

    2010-01-01

    No texto o autor apresenta os temas que foram apresentados e discutidos no X PANAM, Congreso Panamericano de Ingeniería de Tránsito y Transporte, realizado em setembro de 1998 em Santander, na Espanha.

  8. TR146 cells grown on filters as a model of human buccal epithelium

    DEFF Research Database (Denmark)

    Nielsen, Hanne Mørck; Rassing, M R

    1999-01-01

    (app)) of the hydrophilic marker mannitol due to exposure to solutions with pH values or osmolality values different from the physiological values was studied. As in studies with solutions of either taurocholate (TC), glycocholate (GC) or glycodeoxycholate (GDC) the results were compared to the increase in P......(app) of mannitol obtained in analog studies using porcine buccal mucosa in an Ussing chamber. The effect of the exposure on the electrical resistance of the TR146 cell culture model and the porcine buccal mucosa was measured, and the degree of protein leakage due to GC exposure was investigated in the TR146 cell...... culture model. The porcine buccal mucosa was approximately ten times less permeable to mannitol than the TR146 cell culture model. The P(app)TC. Increased P(app) values correlated with a decrease in the electrical resistance of the TR146 cell culture model and the porcine buccal mucosa. GC was shown...

  9. Datasets from an interaction proteomics screen for substrates of the SCF(βTrCP) ubiquitin ligase

    NARCIS (Netherlands)

    Magliozzi, Roberto; Peng, Mao; Mohammed, Shabaz; Guardavaccaro, Daniele; Heck, Albert J R; Low, Teck Yew

    An affinity purification-mass spectrometry (AP-MS) method was employed to identify novel substrates of the SCF(βTrCP) ubiquitin ligase. A FLAG-HA tagged version of the F-box protein βTrCP2, the substrate recognition subunit of SCF(βTrCP), was used as bait. βTrCP2 wild type and the two mutants

  10. Herpesvirus telomerase RNA (vTR with a mutated template sequence abrogates herpesvirus-induced lymphomagenesis.

    Directory of Open Access Journals (Sweden)

    Benedikt B Kaufer

    2011-10-01

    Full Text Available Telomerase reverse transcriptase (TERT and telomerase RNA (TR represent the enzymatically active components of telomerase. In the complex, TR provides the template for the addition of telomeric repeats to telomeres, a protective structure at the end of linear chromosomes. Human TR with a mutation in the template region has been previously shown to inhibit proliferation of cancer cells in vitro. In this report, we examined the effects of a mutation in the template of a virus encoded TR (vTR on herpesvirus-induced tumorigenesis in vivo. For this purpose, we used the oncogenic avian herpesvirus Marek's disease virus (MDV as a natural virus-host model for lymphomagenesis. We generated recombinant MDV in which the vTR template sequence was mutated from AATCCCAATC to ATATATATAT (vAU5 by two-step Red-mediated mutagenesis. Recombinant viruses harboring the template mutation replicated with kinetics comparable to parental and revertant viruses in vitro. However, mutation of the vTR template sequence completely abrogated virus-induced tumor formation in vivo, although the virus was able to undergo low-level lytic replication. To confirm that the absence of tumors was dependent on the presence of mutant vTR in the telomerase complex, a second mutation was introduced in vAU5 that targeted the P6.1 stem loop, a conserved region essential for vTR-TERT interaction. Absence of vTR-AU5 from the telomerase complex restored virus-induced lymphoma formation. To test if the attenuated vAU5 could be used as an effective vaccine against MDV, we performed vaccination-challenge studies and determined that vaccination with vAU5 completely protected chickens from lethal challenge with highly virulent MDV. Taken together, our results demonstrate 1 that mutation of the vTR template sequence can completely abrogate virus-induced tumorigenesis, likely by the inhibition of cancer cell proliferation, and 2 that this strategy could be used to generate novel vaccine candidates

  11. Light curve analysis of beta Lyrae type binary star EM TrA

    Science.gov (United States)

    Özkardeş, B.

    2017-02-01

    An analysis of photometric observations of the eclipsing binary system EM TrA (TYC 9258-211-1=CD-67 1660) is presented in this study. The V light curve of the system from All Sky Automated Survey (ASAS) was solved using the Wilson-Devinney code. The final solution describes EM TrA as a detached system. The absolute parameters of the components of the system were estimated.

  12. Standardized test for anti-Tr/DNER in patients with paraneoplastic cerebellar degeneration.

    Science.gov (United States)

    Probst, Christian; Komorowski, Lars; de Graaff, Esther; van Coevorden-Hameete, Marleen; Rogemond, Veronique; Honnorat, Jerome; Sabeter, Lidia; Graus, Francesc; Jarius, Sven; Voltz, Raymond; Wildemann, Brigitte; Franciotta, Diego; Blöcker, Inga M; Schlumberger, Wolfgang; Stöcker, Winfried; Sillevis Smitt, Peter A E

    2015-04-01

    To determine sensitivity and specificity of a standardized recombinant cell-based indirect immunofluorescence assay (RC-IFA) for anti-Tr antibodies in comparison to a reference procedure. Delta/Notch-like epidermal growth factor-related receptor (DNER) was expressed in HEK293 and used as a substrate for RC-IFA. HEK293 control cells expressing CDR2/Yo and CDR2L as well as mock-transfected HEK293 cells were used as controls. Serum samples from 38 patients with anti-Tr antibodies (33 with paraneoplastic cerebellar degeneration [PCD] and Hodgkin lymphoma), 66 patients with anti-Tr-negative PCD, 53 patients with Hodgkin lymphoma without neurologic symptoms, 40 patients with rheumatic diseases, and 42 healthy blood donors were tested for anti-DNER reactivity in the RC-IFA. In addition, RC-IFA results were compared to those from a commercial tissue-based IFA using monkey cerebellum. Using the RC-IFA, anti-DNER was detected in all anti-Tr-positive patients but in none of the controls (sensitivity 100%, 95% confidence interval [CI] 92.8%-100%; specificity 100%, 95% CI 98.7%-100%). In comparison, anti-Tr was not detected in 4 samples with low-titer autoantibodies using the commercial tissue-based assay. Preadsorption of sera with either recombinant full-length DNER or its extracellular domain selectively abolished anti-Tr reactivity. Anti-Tr antibodies bind to the extracellular domain of DNER and can be detected by RC-IFA using HEK293 cells expressing the recombinant receptor. The new method performs better than a frequently used commercial tissue-based indirect immunofluorescence assay (IFA) in samples with low-titer antibodies. This study provides Class II evidence that RC-IFA accurately detects anti-Tr as compared to conventional IFA.

  13. Kirjastamine 21. sajandil - trüki oma raamat ise / Rivo Sarapik, Andres Kütt, Stanislav Sinyagin

    Index Scriptorium Estoniae

    Sarapik, Rivo, 1981-

    2008-01-01

    Väikesetiraa̓iliste trükiste kirjastamise etappidest ja soovitusi trükifirmade valikul internetis ning erinevate inimeste kogemusi fotoraamatu või fotoajakirja trükkimisel, kasutades Blurb.com või Lulu.com teenust. Kuni väljatrükini on teenus valdavalt tasuta

  14. 77 FR 73456 - Update to the TR-12 Fuel Related Rate Adjustment Policy (SDDC Fuel Surcharge Policy)

    Science.gov (United States)

    2012-12-10

    ... Department of the Army Update to the TR-12 Fuel Related Rate Adjustment Policy (SDDC Fuel Surcharge Policy...: Reference: TR-12 Fuel Related Rate Adjustment Policy. Background: The following FRA policy applies to... increases in diesel fuel prices. Miscellaneous: A copy of the TR-12 FRA Policy can be accessed via the SDDC...

  15. Leser-Trélat syndrome in patients affected by six multiple metachronous primitive cancers

    Directory of Open Access Journals (Sweden)

    Giannetti Alberto

    2010-01-01

    Full Text Available Abstract Leser-Trélat syndrome is characterized by the eruptive appearance of multiple seborrheic keratoses in association with underlying malignant disease. Usually, the sign of Leser-Trélat is associated with adenocarcinoma, most frequently of the colon, breast, or stomach, but also of the lung, kidney, liver, and pancreas. Herein, we present a case that we believe is the first report of the sign of Leser-Trélat in association with occult gastric adenocarcinoma and the anamnestic oncologic history of five other multiple primitive cancers. Epidermal growth factor receptor (EGFR immunohistochemical expression analysis of multiple seborrheic keratoses revealed an intense membranous staining in the basal keratinocytes and in all the upper epidermal layers. Patients with the sign of Leser-Trélat should undergo a diagnostic screening programme for malignant disease along with patients with known Leser-Trélat syndrome who present with a recent acute and florid eruption of their seborrheic keratoses. We propose the importance of combining the molecular features of multiple seborrheic keratoses with EGFR immunohistochemistry analyses to determine the likelihood of Leser-Trélat syndrome and the consequent high risk of underlying multiple visceral malignancies.

  16. TR-BREATH: Time-Reversal Breathing Rate Estimation and Detection.

    Science.gov (United States)

    Chen, Chen; Han, Yi; Chen, Yan; Lai, Hung-Quoc; Zhang, Feng; Wang, Beibei; Liu, K J Ray

    2017-04-28

    In this paper, we introduce TR-BREATH, a timereversal (TR) based contact-free breathing monitoring system. It is capable of breathing detection and multi-person breathing rate estimation within a short period of time using off-the-shelf WiFi devices. The proposed system exploits the channel state information (CSI) to capture the miniature variations in the environment caused by breathing. To magnify the CSI variations, TRBREATH projects CSIs into the TR resonating strength (TRRS) feature space and analyzes the TRRS by the Root-MUSIC and affinity propagation algorithms. Extensive experiment results indoor demonstrate a perfect detection rate of breathing. With only 10 seconds of measurement, a mean accuracy of 99% can be obtained for single-person breathing rate estimation under the non-line-of-sight (NLOS) scenario. Furthermore, it achieves a mean accuracy of 98:65% in breathing rate estimation for a dozen people under the line-of-sight (LOS) scenario and a mean accuracy of 98:07% in breathing rate estimation of 9 people under the NLOS scenario, both with 63 seconds of measurement. Moreover, TR-BREATH can estimate the number of people with an error around 1. We also demonstrate that TR-BREATH is robust against packet loss and motions. With the prevailing of WiFi, TR-BREATH can be applied for in-home and real-time breathing monitoring.

  17. Azole-resistant Aspergillus fumigatus harboring TR34/L98H, TR46/Y121F/T289A and TR53 mutations related to flower fields in Colombia

    Science.gov (United States)

    Alvarez-Moreno, Carlos; Lavergne, Rose-Anne; Hagen, Ferry; Morio, Florent; Meis, Jacques F.; Le Pape, Patrice

    2017-03-01

    Resistance to triazoles in Aspergillus fumigatus has been reported in azole-naive patients in Europe, Asia, Australia and North America. This resistance has been linked to fungicide-driven mutations in the cyp51A gene and its promoter region. We investigated the presence of environmental azole-resistant A. fumigatus strains related to the use of azole fungicides in Colombia. Soil samples were collected from flower beds, flower fields and public gardens from the outskirts, suburbs and city centre of Bogotá. Out of the 86 soil samples taken, 17 (19.8%) grew A. fumigatus of whom eight (9.3%) contained 40 strains able to grow on azole-containing itraconazole and/or voriconazole supplemented media. All but one triazole-resistant strains were isolated from soil samples collected from flower fields and flower beds (39/40). Importantly, the majority had the TR46/Y121F/T289A, TR34/L98H, and TR53 molecular resistance mechanisms and one azole resistant strain had a wild-type cyp51A gene. Soil samples from flower fields and beds contained 4 azole fungicides (penconazole, difenoconazole, tetraconazole and tebuconazole) above the limit of detection. Our findings underline the need for extensive investigations to determine azole-resistant A. fumigatus prevalence in both clinical and environmental samples in other regions of Latin America.

  18. Near-UV and optical observations of the transiting exoplanet TrES-3b

    Science.gov (United States)

    Turner, Jake D.; Smart, Brianna M.; Hardegree-Ullman, Kevin K.; Carleton, Timothy M.; Walker-LaFollette, Amanda M.; Crawford, Benjamin E.; Smith, Carter-Thaxton W.; McGraw, Allison M.; Small, Lindsay C.; Rocchetto, Marco; Cunningham, Kathryn I.; Towner, Allison P. M.; Zellem, Robert; Robertson, Amy N.; Guvenen, Blythe C.; Schwarz, Kamber R.; Hardegree-Ullman, Emily E.; Collura, Daniel; Henz, Triana N.; Lejoly, Cassandra; Richardson, Logan L.; Weinand, Michael A.; Taylor, Joanna M.; Daugherty, Michael J.; Wilson, Ashley A.; Austin, Carmen L.

    2013-01-01

    We observed nine primary transits of the hot Jpiter TrES-3b in several optical and near-UV photometric bands from 2009 June to 2012 April in an attempt to detect its magnetic field. Vidotto, Jardine and Helling suggest that the magnetic field of TrES-3b can be constrained if its near-UV light curve shows an early ingress compared to its optical light curve, while its egress remains unaffected. Predicted magnetic field strengths of Jpiter-like planets should range between 8 G and 30 G. Using these magnetic field values and an assumed B* of 100 G, the Vidotto et al. method predicts a timing difference of 5-11 min. We did not detect an early ingress in our three nights of near-UV observations, despite an average cadence of 68 s and an average photometric precision of 3.7 mmag. However, we determined an upper limit of TrES-3b's magnetic field strength to range between 0.013 and 1.3 G (for a 1-100 G magnetic field strength range for the host star, TrES-3) using a timing difference of 138 s derived from the Nyquist-Shannon sampling theorem. To verify our results of an abnormally small magnetic field strength for TrES-3b and to further constrain the techniques of Vidotto et al., we propose future observations of TrES-3b with other platforms capable of achieving a shorter near-UV cadence. We also present a refinement of the physical parameters of TrES-3b, an updated ephemeris and its first published near-UV light curve. We find that the near-UV planetary radius of Rp = 1.386+ 0.248- 0.144 RJup is consistent with the planet's optical radius.

  19. NF-κB inhibitor reverses temozolomide resistance in human glioma TR/U251 cells.

    Science.gov (United States)

    Wang, Xuan; Jia, Lili; Jin, Xiaohua; Liu, Qian; Cao, Wei; Gao, Xiangdong; Yang, Mingfeng; Sun, Baoliang

    2015-06-01

    Glioblastoma multiforme (GBM) demonstrates an unsatisfactory clinical prognosis due to the intrinsic or acquired resistance to temozolomide (TMZ) exhibited by the tumors. One possible cause of TMZ resistance in GBM is the overexpression of O6-methylguanine-DNA methyltransferase (MGMT), which can repair the TMZ-induced guanine damage in DNA. Additionally, excessive activated NF-κB is reported to be a component of the major inflammatory transcription pathway that is associated with TMZ resistance in GBM. However, the association between the NF-κB pathway and MGMT expression in GBM cells is unknown. Therefore, in the present study, the TMZ resistant (TR) U251 cell line (TR/U251) was successfully constructed to detect how the TR/U251 cell line and the parental U251 cell line each interact with TMZ in vitro. The TR/U251 cells were approximately five times more resistant to TMZ compared with the parental cells. Furthermore, it was found that the NF-κB inhibitor BAY 11-7082 suppressed the expression of MGMT in TR/U251 cells and enhanced TMZ-induced cytotoxicity and apoptosis, thereby indicating that the NF-κB pathway and MGMT interact to promote TMZ resistance. The inhibition of NF-κB may be a promising strategy to reverse drug resistance in TR glioma cells. The present results propose a potential mechanism for using the NF-κB inhibitor BAY 11-7082 as a potential therapy for the treatment of TR glioma. Although BAY 11-7082 is a well-known NF-κB inhibitor, the present study further investigated its underlying mechanisms through a series of new experiments.

  20. Dėl keleto retų vietovardžių kilmės (Greikónys, Grìkapėdis, Plasapnỹkai, Pušė́nai, Silgiónys, Tauliùkai, Vaĩsodžiai

    Directory of Open Access Journals (Sweden)

    Jonas Palionis

    2011-12-01

    Full Text Available ZUR ENSTEHUNG EINIGER SELTENER ORTSNAMEN (Greikónys, Grìkapėdis, Plasapnỹkai, Pušė́nai, Silgiónys, Tauliùkai, VaĩsodžiaiZusammenfassungIm vorliegenden Artikel werden sieben seltene Dorfnamen aus dem südlitauischen Gebiet behandelt. Die Analyse der Entstehung dieser Ortsnamen stützt sich vorwiegend auf das handschriftliche Material der Tauf- und Trauungsurkunden der zweiten Hälfte des 17. und der ersten Hälfte des 18. Jhs der Gemeinde Punià, die gegenwärtig dem Bezirks Alytùs angehört.Greikónys, die ältere Form ist bereits 1659 in einer Taufurkunde verzeichnet (de Gireykancow; das scheint eine Ableitung von dem Familiennamen *Gireika zu sein. Dieser Name läßt sich mit dem Verb gìrti ‘rühmen, loben’ in Verbindung setzen: *gireika- einer, der eine besondere Neigung sich zu rühmen aufweist, Prahlhans ist.Grìkapėdis ist, ohne Zweifel, ebenfalls anthroponymischen Ursprungs, da der Familienname Grìkpėdis im nordwestlichen Teil von Žemaitija vorkommt. Die Bedeutung der Komponenten dieses zusammengesetzten Familiennamens (grìkas ‘Buchweizen’ + pė́das ‘die Getreidegarbe’ zeigt eine metaphorische Basis der Entstehung des Kompositums. Die gegenwärtige Singularform ist wahrscheinlich eine spätere Bildung: in den Taufurkunden der zweiten Hälfte des 17. Jhs findet man Pluralformen (de Grikopedziow, de Grikiepedzow.Plasapnỹkai, änlich wie Greikonys und Grikapėdis, läßt ebenfalls einen anthroponymischen Ursprung vermuten, obwohl dies nicht so augenscheinlich ist. Dieser Ortsname konnte vom Familiennamen *Plasapnỹkas entstanden sein: *Plasapnỹkas < *plasa +  *upinykas, d. h. ‘ein an dem Fluß Plasãupė Wohnender’. Das erste Glied dieser kontrahierter Zusammensetzung könnte man auf eine Form des Verbs plàsti ‘breiter werden’ zurückführen.Pušė́nai kommt in der zweiten Hälfte des 17. und der ersten Hälfte des 18. Jhs als Puišėnai (de Puyszany vor. Diese ältere Form weist

  1. High-efficiency L-band T/R Module: Development Results

    Science.gov (United States)

    Edelstein, Wendy N.; Andricos, Constantine; Wang, Feiyu; Rutled, David B.

    2005-01-01

    Future interferometric synthetic aperture radar (InSAR) systems require electronically scanned phased-array antennas, where the transmit/receive (T/R) module is a key component. The T/R module efficiency is a critical figure of merit and has direct implications on the power dissipation and power generation requirements of the system. Significant improvements in the efficiency of the T/R module will make SAR missions more feasible and affordable. The results of two high-efficiency T/R modules are presented, each based on different power amplifier technologies. One module uses a 30W GaAs Class-AlB power amplifier and the second module uses a 70W LD-MOS Class-ElF power amplifier, where both modules use a common low power section. Each module operates over an 80MHz bandwidth at L-band (1.2GHz) with an overall module efficiency greater than 58%. We will present the results of these two T/R modules that have been designed, built and tested.

  2. Spanish Validation of the School Anxiety Scale-Teacher Report (SAS-TR).

    Science.gov (United States)

    Orgilés, Mireia; Fernández-Martínez, Iván; Lera-Miguel, Sara; Marzo, Juan Carlos; Medrano, Laura; Espada, José Pedro

    2017-10-01

    This study aimed to examine the factorial structure and psychometric properties of the School Anxiety Scale-Teacher Report (SAS-TR) in a community sample of 315 Spanish children aged 5 to 12 years. Thirty-seven teachers from eleven schools completed the SAS-TR and the Strengths and Difficulties Questionnaire (SDQ) for each child. Confirmatory factor analysis supported the original two-factor structure, but a better fit model was obtained after removing four items. The scale was found to have high internal consistency (α = 0.91) and satisfactory test-retest reliability (ICC = 0.87) for the Spanish sample. Convergent validity was supported by positive significant correlations between the SAS-TR and the Emotional Symptoms subscale of the SDQ. Lower correlations between the SAS-TR and the SDQ Conduct Problems subscale supported the divergent validity. Overall, the findings suggest that the Spanish version of the SAS-TR is a reliable and valid instrument for teachers to assess anxiety in Spanish children.

  3. Nano-structured variable capacitor based on P(VDF-TrFE) copolymer and carbon nanotubes

    Science.gov (United States)

    Lakbita, I.; El-Hami, K.

    2018-02-01

    A newly organic capacitor was conceived with a variable capacitance using the inverse piezoelectric effect. The device consists of two parallel plates of carbon nanotubes (CNTs), known for their large surface area, high sensitivity and high electric conductivity, separated by a thin film of a dielectric layer of Polyinylidene fluoride and trifluoroehtylene (P(VDF-TrFE)) promising material for piezoelectric and ferroelectric properties. The obtained architecture is the CNT/PVDF-TrFE/CNT capacitor device. In this study, an ultra-thin film of P(VDF-TrFE) (54/46) with thickness of 20 nm was elaborated on highly oriented pyrolytic graphite (HOPG) by spin-coating. The morphology of the ultra-thin film and the mechanical behavior of CNT/P(VDF-TrFE)/CNT system were studied using the atomic force microscopy (AFM) combined with a lock-in amplifier in contact mode. All changes in applied voltage induce a change in thin film thickness according to the inverse piezoelectric effect that affect, consequently the capacitance. The results showed that the ratio of capacitance change ΔC to initial capacitance C0 is ΔC/C0=5%. This value is sufficient to use P(VDF-TrFE) as variable organic capacitor.

  4. Reconstruction of sound source signal by analytical passive TR in the environment with airflow

    Science.gov (United States)

    Wei, Long; Li, Min; Yang, Debin; Niu, Feng; Zeng, Wu

    2017-03-01

    In the acoustic design of air vehicles, the time-domain signals of noise sources on the surface of air vehicles can serve as data support to reveal the noise source generation mechanism, analyze acoustic fatigue, and take measures for noise insulation and reduction. To rapidly reconstruct the time-domain sound source signals in an environment with flow, a method combining the analytical passive time reversal mirror (AP-TR) with a shear flow correction is proposed. In this method, the negative influence of flow on sound wave propagation is suppressed by the shear flow correction, obtaining the corrected acoustic propagation time delay and path. Those corrected time delay and path together with the microphone array signals are then submitted to the AP-TR, reconstructing more accurate sound source signals in the environment with airflow. As an analytical method, AP-TR offers a supplementary way in 3D space to reconstruct the signal of sound source in the environment with airflow instead of the numerical TR. Experiments on the reconstruction of the sound source signals of a pair of loud speakers are conducted in an anechoic wind tunnel with subsonic airflow to validate the effectiveness and priorities of the proposed method. Moreover the comparison by theorem and experiment result between the AP-TR and the time-domain beamforming in reconstructing the sound source signal is also discussed.

  5. Excess Ca(2+) does not alleviate but increases the toxicity of Hg(2+) to photosystem II in Synechocystis sp. (Cyanophyta).

    Science.gov (United States)

    Zhang, Daoyong; Deng, Chunnuan; Pan, Xiangliang

    2013-11-01

    This study demonstrated that excess Ca(2+) increased the toxicity of Hg(2+) to PSII of cyanobacterium Synechocystis sp. using fast rise chlorophyll fluorescence test. Excess Ca(2+) increased the inhibitory effect of Hg(2+) on O2 evolution. Exposure to Hg(2+) caused increase in functional antenna size (ABS/RC), trapping rate of reaction center (TR0/RC), dissipated energy flux per reaction center (DI0/RC) and maximum quantum yield of non-photochemical deexcitation ( [Formula: see text] ), indicating that some reaction centers were transformed to dissipation sinks under Hg(2+) stress. Hg(2+) stress slowed down electron transport on both donor side and acceptor side and caused accumulation of P680(+). Excess Ca(2+) intensified all the Hg(2+) toxic effects on PSII function and led to dysfunction of PSII. The number of reaction centers that were transformed into dissipation sinks increased with increasing Ca(2+) concentration. Copyright © 2013 Elsevier Inc. All rights reserved.

  6. Avaliação dos parametros termo-fisicos e cinetica de congelamento de cação

    OpenAIRE

    Haiko Hense

    1990-01-01

    Resumo: Foram determinadas experimentalmente curvas de congelamento de filé de cação (Machote, Anjo, Manteiga e Asul), em um sistema que simulava um congelador a placas. Ele consistia em um módulo que possibilitava transferência de calor unidirecional através de uma face não isolada termicamente, submersa no banho de um criostato, mantido a três níveis de temperatura, -25, -35 e -45 ºC. A carne de cação, crua ou cosida, com um contendo de umidade em base úmida na faixa entre 73,5% e 85%, era ...

  7. Ultrasonic investigation close to quantum critical point in YbTr{sub 2}Zn{sub 20} (Tr: Co, Rh, and Ir)

    Energy Technology Data Exchange (ETDEWEB)

    Nakanishi, Y; Kamiyama, T; Ito, K; Nakamura, M; Yoshizawa, M [Graduate School of Engineering, Iwate University, Morioka 020-8551 (Japan); Saiga, Y [Graduate School of Advanced Sciences of Matter, Hiroshima University, HigashiHiroshima 739-8530 (Japan); Kosaka, M [Department of Physics, Saitama University, Saitama 338-8570 (Japan); Uwatoko, Y, E-mail: yoshiki@iwate-u.ac.j [Institute for Solid State Physics, University of Tokyo, Kashiwa 227-8581 (Japan)

    2010-01-15

    We performed ultrasonic measurements on high quality single crystals of the Yb-based heavy fermion compounds YbTr{sub 2}Zn{sub 20} (Tr: Co, Rh and Ir) over a temperature range from 200 K to 0.5 K, which seem to be close to a quantum critical point (QCP). A sharp contrast of the temperature dependence of elastic constants was found at low temperature among the three compounds, reflecting the 4f electronic state stemmed from Yb ion. The results indicate that a crystalline electric field (CEF) effect seems to be dominant in the systems YbRh{sub 2}Zn{sub 20} and YbIr{sub 2}Zn{sub 20} at low temperatures. On the other hand, the CEF effect is much less, but an additional effect would be dominant which is most probably ascribable to non Fermi liquid characteristics formed close to the QCP. We discuss briefly each 4f electronic state developed at the low temperatures and physical parameters relating to a renormalized band model in YbTr{sub 2}Zn{sub 20} in the framework of a deformation potential approximation.

  8. Clinical Significance of Serum HE4, CA125, CA724, and CA19-9 in Patients With Endometrial Cancer.

    Science.gov (United States)

    Bian, Jing; Sun, Xiaoxu; Li, Bo; Ming, Liang

    2017-08-01

    Serum markers with increased sensitivity and specificity for endometrial cancer are required. To date, no good marker has met this standard. The aims of our study were to evaluate the utility of tumor markers HE4, CA125, CA724, and CA19-9 as potential markers in patients diagnosed with endometrial cancer. Blood samples from 105 patients with endometrial cancer and 87 healthy women were analyzed by Roche electrochemiluminescent immunoassay, and serum values were measured for the following biomarkers: HE4, CA125, CA724, and CA19-9. Serum HE4, CA125, CA724, and CA19-9 concentrations were significantly higher in patients with endometrial cancer, compared with controls ( P endometrial cancer, HE4 had higher sensitivity (58%), positive predictive value (60%), and negative predictive value (67%) than any other single tumor marker, and in the combination of HE4, CA125, CA724, and CA19-9, the sensitivity and positive predictive values reached 59.1% and 88%, respectively. Meanwhile, the receiver operating characteristic area under the curve of the combination of the 4 markers was significantly increased than any other group, either in stage I or in stage II to IV cases. HE4 and CA125 both correlate with advanced age; in addition, HE4 was related to pathology subtypes and positive adnexal involvement, CA125 was related to International Federation of Gynecology and Obstetrics stage, CA19-9 was related to International Federation of Gynecology and Obstetrics stage, and CA724 was correlated with positive lymph node. Combination of HE4, CA125, CA724, and CA19-9 has the highest value in diagnosing endometrial cancer, and they can be a useful tissue immune marker for patients with endometrial cancer.

  9. Descobertas de exoplanetas pelo método do trânsito

    OpenAIRE

    Santos, W.C.; Amorim, R. G. G.

    2017-01-01

    Apresentamos neste trabalho uma breve revisão sobre o método de trânsito na detecção de exoplanetas. O trânsito é uma técnica baseada na redução do brilho de uma estrela quando o exoplaneta passa na sua frente percorrendo o seu disco, com respeito à linha de visada. O método de trânsito é responsável por 23% das detecções de exoplanetas. Nesse sentido, discutimos de forma pedagógica os detalhes da técnica, deduzindo as principais equações. Por fim, a aplicação do método na detecção do exoplan...

  10. Psychometric Properties of the Lesbian, Gay, and Bisexual Identity Scale-Turkish (LGBIS-TR).

    Science.gov (United States)

    Kemer, Gülşah; Demirtaş, Ezgi Toplu; Pope, Amber L; Ummak, Esra

    2017-01-01

    The aim of the current study was to examine the psychometric properties of the Lesbian, Gay, and Bisexual Identity Scale (LGBIS; Mohr & Kendra, 2011) in a Turkish sample. Three hundred and ten LGB individuals participated and completed the Turkish version of the LGBIS (LGBIS-TR) along with the Satisfaction with Life, Positive and Negative Affect, and Self-Compassion Scales. Confirmatory factor analysis results yielded a good-fit for eight subscales of the LGBIS-TR. We also obtained satisfactory criterion validity and internal consistency reliability. Based on this initial study, the LGBIS-TR appears to be a psychometrically sound instrument to gauge LGB individuals' identity experiences in Turkey. We discuss the results along with previous findings and limitations of the study, and we provide implications for further research and practice.

  11. Fysisk aktivitet og træning til patienter i hæmodialysebehandling

    DEFF Research Database (Denmark)

    Dè Hòra, Nette Solskov; Brincks, Ulrikke Kodal; Steffensen, Ida Toftlund

    træningskonceptet blandt patienter i HD og personale. Fysisk funktionsevne (evnen og kapaciteten til at udføre hverdagslivets aktiviteter) er alvorligt reducerede hos patienter med kronisk nyresvigt, og studier har påvist en reduktion på 50 % af den forventede kapacitet ved start på dialyse behandling. Fysisk...... informere og støtte alle patienter med kronisk nyresvigt i at gennemføre fysisk træning. På trods af evidens for effekten af fysisk træning og aktivitet for patienter med kronisk nyresvigt i dialyse, er det kun indført i enkelte danske dialyseafdelinger. Projektet er designet inden for det britiske Medical...

  12. Personalidad e infracciones frecuentes de normas de tránsito

    Directory of Open Access Journals (Sweden)

    Nicolasa María Durán Palacio

    2016-01-01

    Full Text Available La Organización Mundial de la Salud (2013, alerta a los gobiernos a tomar medidas urgentes para prevenir accidentes de tránsito. Si no se intervienen, en el 2030 serán la quinta causa de muerte. Este estudio establece el perfil psicológico de conductores de vehículo infractores frecuentes de normas de tránsito, en una muestra de 295 adultos de ambos sexos. Utilizando el cuestionario factorial de personalidad 16PF-5 y una escala de creencias, conocimientos, y comportamientos sobre seguridad vial, se identificaron relaciones significativas entre conducta infractora y creencias, además de los factores de personalidad y variables específicas que predicen la propensión a las infracciones frecuentes de normas de tránsito.

  13. Tráfico de drogas: uma opção entre escolhas escassas

    Directory of Open Access Journals (Sweden)

    Ana Amélia Cypreste Faria

    2011-12-01

    Full Text Available Este trabalho procura compreender os aspectos psicossociais que permeiam a adesão de pessoas ao tráfico de drogas em seu contexto histórico e econômico-social, por meio de pesquisa realizada no ambiente carcerário, onde foram recolhidas histórias de vida de pessoas envolvidas com o tráfico. Em um ambiente socioeconômico caracterizado pela precarização das relações de trabalho, pelo desemprego e pelo apelo consumista afinados com as premissas econômicas neoliberais tem-se uma situação de exclusão social e de cidadania. Assim, o tráfico se mostra como uma atividade econômica possibilitadora de inclusão, mesmo que marginal, na ordem capitalista. Uma opção a ser feita entre escolhas limitadas.

  14. Comparing Diagnostic Outcomes of Autism Spectrum Disorder Using DSM-IV-TR and DSM-5 Criteria.

    Science.gov (United States)

    Harstad, Elizabeth B; Fogler, Jason; Sideridis, Georgios; Weas, Sarah; Mauras, Carrie; Barbaresi, William J

    2015-05-01

    Controversy exists regarding the DSM-5 criteria for ASD. This study tested the psychometric properties of the DSM-5 model and determined how well it performed across different gender, IQ, and DSM-IV-TR sub-type, using clinically collected data on 227 subjects (median age = 3.95 years, majority had IQ > 70). DSM-5 was psychometrically superior to the DSM-IV-TR model (Comparative Fit Index of 0.970 vs 0.879, respectively). Measurement invariance revealed good model fit across gender and IQ. Younger children tended to meet fewer diagnostic criteria. Those with autistic disorder were more likely to meet social communication and repetitive behaviors criteria (p DSM-5 is a robust model but will identify a different, albeit overlapping population of individuals compared to DSM-IV-TR.

  15. Vloga grafičnega oblikovanja v tržni komunikaciji

    OpenAIRE

    Praznik, Daša

    2015-01-01

    Urejena celostna grafična podoba je tisti del trženja, ki na naše poslovne partnerje, potrošnike naredi prvi vtis. Pravijo, da obleka naredi človeka in tako je tudi z grafičnim oblikovanjem ter trženjem. Uspešno grafično oblikovanje, uporaba vizualnih komunikacij nam omogoči komunikacijo, ki naš produkt predstavi publiki v umetniški luči in poskrbi za bolj topel odnos. Tega se tržno komuniciranje še kako dobro zaveda, saj povezanost z družbo in s tem kulturo pripelje do dobrih rezultatov in d...

  16. The design of an asynchronous Tiny RISC TM/TR4101 microprocessor core

    DEFF Research Database (Denmark)

    Christensen, Kåre Tais; Jensen, P.; Korger, P.

    1998-01-01

    This paper presents the design of an asynchronous version of the TR4101 embedded microprocessor core developed by LSI Logic Inc. The asynchronous processor, called ARISC, was designed using the same CAD tools and the same standard cell library that was used to implement the TR4101. The paper...... reports on the design methodology, the architecture, the implementation, and the performance of the ARISC. This includes a comparison with the TR4101, and a detailed breakdown of the power consumption in the ARISC. ARISC is our first attempt at an asynchronous implementation and a number of simplifying...... decisions were made up front. Throughout the entire design we use four-phase handshaking in combination with a normally opaque latch controller. All logic is implemented using static logic standard cells. Despite this the ARISC performs surprisingly well: In 0.35 μm CMOS performance is 74-123 MIPS depending...

  17. Regulation of mitosis-meiosis transition by the ubiquitin ligase β-TrCP in male germ cells.

    Science.gov (United States)

    Nakagawa, Tadashi; Zhang, Teng; Kushi, Ryo; Nakano, Seiji; Endo, Takahiro; Nakagawa, Makiko; Yanagihara, Noriko; Zarkower, David; Nakayama, Keiko

    2017-11-15

    The mitosis-meiosis transition is essential for spermatogenesis. Specific and timely downregulation of the transcription factor DMRT1, and consequent induction of Stra8 expression, is required for this process in mammals, but the molecular mechanism has remained unclear. Here, we show that β-TrCP, the substrate recognition component of an E3 ubiquitin ligase complex, targets DMRT1 for degradation and thereby controls the mitosis-meiosis transition in mouse male germ cells. Conditional inactivation of β-TrCP2 in male germ cells of β-TrCP1 knockout mice resulted in sterility due to a lack of mature sperm. The β-TrCP-deficient male germ cells did not enter meiosis, but instead underwent apoptosis. The induction of Stra8 expression was also attenuated in association with the accumulation of DMRT1 at the Stra8 promoter in β-TrCP-deficient testes. DMRT1 contains a consensus β-TrCP degron sequence that was found to bind β-TrCP. Overexpression of β-TrCP induced the ubiquitylation and degradation of DMRT1. Heterozygous deletion of Dmrt1 in β-TrCP-deficient spermatogonia increased meiotic cells with a concomitant reduction of apoptosis. Collectively, our data indicate that β-TrCP regulates the transition from mitosis to meiosis in male germ cells by targeting DMRT1 for degradation. © 2017. Published by The Company of Biologists Ltd.

  18. Conjugal Transfer of Bacteriophage Resistance Determinants on pTR2030 into Streptococcus cremoris Strains.

    Science.gov (United States)

    Sing, W D; Klaenhammer, T R

    1986-06-01

    Agar surface conjugal matings were used to introduce heat-sensitive phage resistance (Hsp) determinants carried on the conjugal plasmid pTR2030 into Streptococcus cremoris KH, HP, 924, and TDM1. Lactose-fermenting (Lac) transconjugants were selected from matings of Lac variants of S. cremoris KH, HP, 924, and TDM1 with Streptococcus lactis ME2 or a high-frequency donor, S. lactis T-EK1 (pTR1040, Lac; pTR2030, Hsp). For all of the S. cremoris strains examined, select Lac transconjugants were completely resistant to plaquing by their homologous lytic phages. In all cases the plaquing efficiencies were less than 10. Acquisition of a 30-megadalton plasmid (pTR2030) in the S. cremoris phage-resistant transconjugants was demonstrated by direct plasmid analysis, by hybridization with P-labeled probes, or by conjugal transfer of pTR2030 out of the phage-resistant transconjugants into a plasmid-cured recipient, S. lactis LM2302. Acid production, coagulation ability, and proteolytic activity of phage-resistant transconjugants in milk were comparable to those of their phage-sensitive parents. Further, S. cremoris phage-resistant transconjugants were not attacked by phage in starter culture activity tests, which included a 40 degrees C incubation period. The results demonstrated that phage resistance determinants on pTR2030 could be conjugally transferred to a variety of S. cremoris strains and confer resistance to phage under conditions encountered during cheese manufacture. Phage-resistant transconjugants of S. cremoris M43 and HP were also constructed without the use of antiblotic markers to select conjugal recipients from mating mixtures.

  19. Solid State compatibilities in CaO-CaO∙Al2O3-CaF2 system

    Directory of Open Access Journals (Sweden)

    Jiménez Molina, S.

    1998-03-01

    Full Text Available In open atmosphere the two phases containing fluorine, C11A7∙CaF2 and 3CA∙CaF2, laying in the CaO-CaO∙Al2O3-CaF2 were synthesized. The synthesis of the 3CA∙CaF2 at 1.100°C was possible without loss of F, when the stoichiometric mixture of CA and CaF2 was pressed into tablets and was applied vacuum, avoiding in such a way the presence of moisture. According to TGA studies, the vapour pressure of phases containing fluorine, at 1.100°C is very low and follows this sequence: CaF2 2 11A7∙CaF2. At 1.000°C the system behaves as condensated. Verification of some compatibilities in solid state in the system CaO-CaO∙Al2O3-CaF2 at 1000°C has been done. Compatibility triangles found are the same described by Chaterjee, and different from the compatibilities given by Brisi and Rolando.Se han sintetizado en atmósfera abierta las dos fases fluoradas del sistema CaO-CaO∙Al2O3-CaF2: C11A7∙CaF2 y 3CA∙CaF2. La síntesis del 3CACaF2 a 1.100°C ha sido posible sin pérdida de F, cuando la mezcla estequiométrica de CA y CaF2 se empastilló a presión y aplicando vacío, como modo de evitar la presencia de humedad. De acuerdo con los resultados de Análisis Termogravimétrico, la presión de vapor de las fases fluoradas, a 1.100°C, es muy baja y sigue el orden: CaF2 2 11A7∙CaF2. A 1.000°C, el sistema se comporta como condensado. Se han verificado algunas relaciones de fases compatibles en estado sólido, en el sistema CaO-CaO∙Al2O3-CaF2, a 1.000°C. Las relaciones de fases compatibles que se han encontrado coinciden con aquéllas descritas por Chaterjee, y son diferentes a las encontradas por Brisi y Rolando.

  20. Los puertos del Estado y el tráfico de cruceros marítimos

    Directory of Open Access Journals (Sweden)

    Estepa Montero, Manuel

    2013-01-01

    Full Text Available El presente artículo analiza la realidad del sector mundial de cruceros en relación con los conceptos de puerto y de tráfico marítimo quea coge la ley de puertos. Describe la evolución del tráfico de cruceros en Norteamérica y Europa. Su incidencia en los puertos españoles y su influenciaen el crecimiento del sector turístico español

  1. Costes de operación del tráfico en la carretera

    OpenAIRE

    Cañadas Salas, Javier

    2007-01-01

    El coste de operación del tráfico en la carretera es el parámetro fundamental que se debe evaluar para la selección de rutas y para comprobar la eficiencia económica de un itinerario. El coste de operación se define como el conjunto de costes que genera el tráfico al circular por un trazado determinado. Los costes se dividen en tres subgrupos. El primer grupo se denomina coste directo, y engloba el coste por tiempo de recorrido, el coste por consumo de combustible y el coste de lo...

  2. A Case of Leser-Trélat Syndrome Associated with a Renal Cell Carcinoma

    Directory of Open Access Journals (Sweden)

    Dietrich Barth

    2015-09-01

    Full Text Available Seborrheic keratoses can often be found in elderly people. In general, they appear gradually. In cases of a sudden eruption with itching it might be paraneoplastic. Although some authors doubt the existence of the paraneoplastic Leser-Trélat syndrome, we present a case of sudden eruption of seborrheic keratoses connected with a newly diagnosed renal cell carcinoma. As far as we know, this is the first case report of a Leser-Trélat syndrome with a malignancy of the kidney.

  3. An introduction to TR-X: a simplified tool for standardized analysis

    Energy Technology Data Exchange (ETDEWEB)

    Johns, Russell C [Los Alamos National Laboratory; Waters, Laurie S [Los Alamos National Laboratory; Fallgren, Andrew J [Los Alamos National Laboratory; Ghoreson, Gregory G [UNIV OF TEXAS

    2010-09-09

    TR-X is a multi-platform program that provides a graphical interface to Monte Carlo N-Particle transport (MCNP) and Monte Carlo N-Particle transport eXtended (MCNPX) codes. Included in this interface are tools to reduce the tedium of input file creation, provide standardization of model creation and analysis, and expedite the execution of the created models. TR-X provides tools to make the rapid testing of multiple permutations of these models easier, while also building in standardization that allows multiple solutions to be compared.

  4. Sistem Peringatan Dini untuk Mendeteksi Lifetime Sparepart pada Mesin TR OSAWA

    Directory of Open Access Journals (Sweden)

    Adi Nugroho

    2017-10-01

    Full Text Available Maintenance is a very important activitiy to keep an equipment always at its best condition. This research aims to create an early warning system/ lifetime spare parts detector of Nissin Kogyo Batam (NKB Company’s TR Osawa machine by adopting PLC (Programmable Logic Controller. PLC is used to automatically calculate the lifetime spare parts of TR Osawa machine. PLC will shut down the machine if it detects that lifetime spare parts have reached the specified setting standard (380-400 k pcs. As a result, PLC machine can provide more accurate results than manual counting.

  5. Nichos tróficos em dípteros caliptrados, no Rio de Janeiro, RJ

    OpenAIRE

    d'ALMEIDA, J. M.; ALMEIDA, J. R.

    1998-01-01

    Foram calculadas a extensão e sobreposição de nichos tróficos de dípteros caliptrados, criados em variados ambientes do Rio de Janeiro (área rural, urbana e florestal), utilizando-se diferentes substratos de criação (banana amassada, carcaça de camundongo, peixe (sardinha), fígado bovino, camarão e fezes humanas frescas. Foram criadas 14.294 moscas, pertencentes às famílias Calliphoridae, Fanniidae, Muscidae e Sarcophagidae. As maiores sobreposições de nichos tróficos, entre as espécies, fora...

  6. Adopting Results Based Management in the Non-Profit Sector: Trócaire’s Experience

    Directory of Open Access Journals (Sweden)

    Eoghan McKernan

    2016-11-01

    Full Text Available The increasing emphasis on aid effectiveness, accountability and   impact measurement in international development and humanitarian work has generated a requirement for high quality internal systems for the management of programmes. To help to address this requirement, Trócaire adopted Results Based Management in the 20 countries in which it works. This paper provides an overview of Trócaire’s RBM journey, including the process of embedding the new approach in the organisation, lessons learnt from this process, the subsequent benefits that are emerging at field programme level and the challenges going forward.

  7. Space-charge-mediated anomalous ferroelectric switching in P(VDF-TrEE) polymer films

    KAUST Repository

    Hu, Weijin

    2014-11-12

    We report on the switching dynamics of P(VDF-TrEE) copolymer devices and the realization of additional substable ferroelectric states via modulation of the coupling between polarizations and space charges. The space-charge-limited current is revealed to be the dominant leakage mechanism in such organic ferroelectric devices, and electrostatic interactions due to space charges lead to the emergence of anomalous ferroelectric loops. The reliable control of ferroelectric switching in P(VDF-TrEE) copolymers opens doors toward engineering advanced organic memories with tailored switching characteristics.

  8. KWU-werkersklasdramas in Afrikaans (ca. 1930 - ca. 1950

    Directory of Open Access Journals (Sweden)

    J. L. Coetser

    1999-05-01

    Full Text Available GWU working class theatre in Afrikaans (ca. 1930 - ca. 1950In 1984 Elsabé Brink drew attention to plays, prose and poetry written between 1930 and 1950 in Afrikaans by members of the Garment Workers’ Union (GWU. Scholars such as Stander and Willemse (1992, Van Niekerk (1996 and Van Wyk (1995, 1997 have also referred to GWU plays. Apart from these overviews, GWU plays as such have not yet received the attention they deserve. This article presents a revaluation, initially by providing an overview of their contents, followed by an examination of cultural, economic and political influences. It is argued that - retrospectively - the GWU plays reflected a unique cultural specificity from the framework established by Sitas (1986 for more contemporary working class theatre.

  9. Betydningen af ”Virtuel Genoptræning” for motivation og fastholdelse af træning

    DEFF Research Database (Denmark)

    Mogensen, Finn Riiskjær; Jakobsen, Pernille

    2016-01-01

    , at helbredet bliver dårligere, og at der ikke er supervision, når genoptræningsforløbet er afsluttet og træningsudstyret hentet. Specifikt i forhold til Virtuel Genoptræning virker det motiverende, at der er feedback og en opfølgning på træningen, samt at borgerne kan tilpasse træningen til egne rutiner...

  10. Raamatute trükkimine : tiraazhid langevad, nimetuste arv kasvab / Margo Kokerov

    Index Scriptorium Estoniae

    Kokerov, Margo, 1978-

    2001-01-01

    Tallinna Raamatutrükikoja tootmisdirektor Valdur Rebane ja tegevdirektor Jüri Simseli trükikodade majanduslikust olukorrast ja hinnapoliitikast. Diagrammid: raamatu 1 eks. väljaandmiskulude sõltuvus tiraazhist. Kommenteerib Silva Tomingas. Erinevate nimetuste, arvu ja kogutiraazhi sõltuvus keskmisest tiraazhist

  11. Integreret ulykkesforebyggelse i træindustrien gennem fokus på kommunikation

    DEFF Research Database (Denmark)

    Pedersen, Louise

    2009-01-01

    interventionsafdeling, mens virksomhed B’s træ- og montageafdeling optræder som kontrolafdelinger. I efteråret 2008 er der gennemført en førmåling af sikkerhedsniveauet i begge virksomheder ud fra både kvantitative (spørgeskemaer, audit og sikkerhedsrunderinger) og kvalitative metoder (interviews, observation og...

  12. Data of evolutionary structure change: 1CERP-2B4TR [Confc[Archive

    Lifescience Database Archive (English)

    Full Text Available 1CERP-2B4TR 1CER 2B4T P R MKVGINGFGRIGRQVFRILHSRG-VEVALINDLT-DNKTLAHLLKYDSIYHRFPGE...1CER P 1CERP LHSRG-VEVAL ...1CER P 1CERP INDLT-DNKTL ...1CER P 1CERP DLPHK---DLRRA...1CER P 1CERP DAKLT-KALGN

  13. Tumor-infiltrating plasmacytoid dendritic cells promote immunosuppression by Tr1 cells in human liver tumors

    NARCIS (Netherlands)

    Pedroza-Gonzalez, A.; Zhou, G.; Vargas-Mendez, E.; Boor, P.P.; Mancham, S.; Verhoef, C.; Polak, W.G.; Grunhagen, D.; Pan, Q.; Janssen, H.; Garcia-Romo, G.S.; Biermann, K.; Tjwa, E.T.; Ijzermans, J.N.M.; Kwekkeboom, J.; Sprengers, D.

    2015-01-01

    CD4+ type 1 T regulatory (Tr1) cells have a crucial role in inducing tolerance. Immune regulation by these cells is mainly mediated through the secretion of high amounts of IL-10. Several studies have suggested that this regulatory population may be involved in tumor-mediated immune-suppression.

  14. Stratigraphie et structure de Trás-os-Montes oriental (Portugal)

    NARCIS (Netherlands)

    Ribeiro, Antonio; Almeida Rebelo, Jose

    1967-01-01

    Rocks in the eastern part of the province of Trás-os-Montes, N. Portugal belong to five units: 1: a complex of pre-Ordovician schists and greywackes; 2: Ordovician and Silurian sediments; 3: a low-grade metamorphic scries of Silurian age; 4: two complexes of probably Precambrian, predominantly meso-

  15. Data for forurenende træer i Europa samlet og tilpasset model for luftforurening

    DEFF Research Database (Denmark)

    Voigt, Steen

    2008-01-01

    Træer forurener. Nogle arter mere end andre. De afgiver både pollen og flere kemiske forbindelser, der påvirker menneskers sundhed - ofte efter transport med vinden over store afstande. Nu har forskere fra Danmarks Miljøundersøgelser ved Aarhus Universitet kortlagt forekomst og udbredelse af 39 t...

  16. August 2003 Registered with Registrc:tr of Newspapers in India

    Indian Academy of Sciences (India)

    Page 1. CPMG/KAlBGE-340/2003-05. Licenced to post WPP(E) NO.6. Resonance - August 2003. YJa~~. (1902 - 1984). Registered with Registrc:tr of Newspapers in India vide Regn. No. 66273/96. ISSN 0971-8044.

  17. Codon Optimization Leads to Functional Impairment of RD114-TR Envelope Glycoprotein

    Directory of Open Access Journals (Sweden)

    Eleonora Zucchelli

    2017-03-01

    Full Text Available Lentiviral vectors (LVs are a highly valuable tool for gene transfer currently exploited in basic, applied, and clinical studies. Their optimization is therefore very important for the field of vectorology and gene therapy. A key molecule for LV function is the envelope because it guides cell entry. The most commonly used in transiently produced LVs is the vesicular stomatitis virus glycoprotein (VSV-G envelope, whose continuous expression is, however, toxic for stable LV producer cells. In contrast, the feline endogenous retroviral RD114-TR envelope is suitable for stable LV manufacturing, being well tolerated by producer cells under constitutive expression. We have previously reported successful, transient and stable production of LVs pseudotyped with RD114-TR for good transduction of T lymphocytes and CD34+ cells. To further improve RD114-TR-pseudotyped LV cell entry by increasing envelope expression, we codon-optimized the RD114-TR open reading frame (ORF. Here we show that, despite the RD114-TRco precursor being produced at a higher level than the wild-type counterpart, it is unexpectedly not duly glycosylated, exported to the cytosol, and processed. Correct cleavage of the precursor in the functional surface and transmembrane subunits is prevented in vivo, and, consequently, the unprocessed precursor is incorporated into LVs, making them inactive.

  18. Validation of a new box trainer-related tracking device: the TrEndo

    NARCIS (Netherlands)

    van Empel, Pieter J.; van Rijssen, Lennart B.; Commandeur, Joris P.; Verdam, Mathilde G. E.; Huirne, Judith A.; Scheele, Fedde; Jaap Bonjer, H.; Jeroen Meijerink, W.

    2012-01-01

    There is an increasing demand for structured objective ex vivo training and assessment of laparoscopic psychomotor skills prior to implementation of these skills in practice. The aim of this study was to establish the internal validity of the TrEndo, a motion-tracking device, for implementation on a

  19. Standardized test for anti-Tr/DNER in patients with paraneoplastic cerebellar degeneration

    NARCIS (Netherlands)

    Probst, Christian; Komorowski, Lars; de Graaff, Esther; van Coevorden-Hameete, Marleen; Rogemond, Veronique; Honnorat, Jerome; Sabeter, Lidia; Graus, Francesc; Jarius, Sven; Voltz, Raymond; Wildemann, Brigitte; Franciotta, Diego; Blöcker, Inga M; Schlumberger, Wolfgang; Stöcker, Winfried; Sillevis Smitt, Peter A E

    OBJECTIVE: To determine sensitivity and specificity of a standardized recombinant cell-based indirect immunofluorescence assay (RC-IFA) for anti-Tr antibodies in comparison to a reference procedure. METHODS: Delta/Notch-like epidermal growth factor-related receptor (DNER) was expressed in HEK293 and

  20. Switching dynamics in ferroelectric P(VDF-TrFE) thin films

    NARCIS (Netherlands)

    Zhao, D.; Katsouras, I.; Asadi, K.; Blom, P.W.M.; Leeuw, D.M. de

    2015-01-01

    Switching, i.e., polarization reversal, of ferroelectric P(VDF-TrFE) thin films has been investigated in a wide range of applied electric field and temperature. The measured polarization transients can be quantitatively described by a compressed exponential function as originally formulated by

  1. Nicotine permeability across the buccal TR146 cell culture model and porcine buccal mucosa in vitro

    DEFF Research Database (Denmark)

    Nielsen, Hanne Mørck; Rassing, Margrethe Rømer

    2002-01-01

    comparable for bi-directional and uni-directional transport in the presence of a transmembrane pH gradient. Nicotine concentrations between 10(-5) and 10(-2) M were applied to the apical side of the TR146 cell culture model or the mucosal side of porcine buccal mucosa. Buffers with pH values of 5.5, 7...

  2. Comparing Personality Disorder Models: Cross-Method Assessment of the FFM and DSM-IV-TR

    Science.gov (United States)

    Samuel, Douglas B.; Widiger, Thomas A.

    2010-01-01

    The current edition of the Diagnostic and Statistical Manual of Mental Disorders (DSM-IV-TR; American Psychiatric Association, 2000) defines personality disorders as categorical entities that are distinct from each other and from normal personality traits. However, many scientists now believe that personality disorders are best conceptualized using a dimensional model of traits that span normal and abnormal personality, such as the Five-Factor Model (FFM). However, if the FFM or any dimensional model is to be considered as a credible alternative to the current model, it must first demonstrate an increment in the validity of the assessment offered within a clinical setting. Thus, the current study extended previous research by comparing the convergent and discriminant validity of the current DSM-IV-TR model to the FFM across four assessment methodologies. Eighty-eight individuals receiving ongoing psychotherapy were assessed for the FFM and the DSM-IV-TR personality disorders using self-report, informant report, structured interview, and therapist ratings. The results indicated that the FFM had an appreciable advantage over the DSM-IV-TR in terms of discriminant validity and, at the domain level, convergent validity. Implications of the findings and directions for future research are discussed. PMID:21158596

  3. Print Best jahib trükiteenuste turul liidrirolli / Toivo Tänavsuu

    Index Scriptorium Estoniae

    Tänavsuu, Toivo

    2004-01-01

    Konkursil Ettevõtluse auhind 2004 piirkonna edendaja nominentide hulka kuuluv Print Best on tõusnud viimase aastaga mahtudelt viie suurema poogentrükikoja hulka Eestis. Firma prioriteediks on Venemaa, mõeldud on müügiüksuse loomisele Moskvas. Lisa: Print Best Trükikoda

  4. Hjemmebaseret fysisk træning for voksne patienter med kronisk hjertesvigt

    DEFF Research Database (Denmark)

    Svensson, Malene; Klenske, Frederikke; Støier, Louise

    Formålet med denne kliniske retningslinje er at undersøge, hvorvidt der er evidens for at hjemmebaseret træning har en gavnlig effekt på det fysiske funktionsniveau og/eller livskvaliteten hos voksne patienter med kronisk hjertesvigt NYHA klasse II-III og dermed kan anbefales til denne...

  5. Deploying the Win TR-20 computational engine as a web service

    Science.gov (United States)

    Despite its simplicity and limitations, the runoff curve number method remains a widely-used hydrologic modeling tool, and its use through the USDA Natural Resources Conservation Service (NRCS) computer application WinTR-20 is expected to continue for the foreseeable future. To facilitate timely up...

  6. Hans Georg Trüper (1936–2016 and His Contributions to Halophile Research

    Directory of Open Access Journals (Sweden)

    Aharon Oren

    2016-05-01

    Full Text Available Prof. Hans Georg Trüper, one of the most important scientists in the field of halophile research, passed away on 9 March 2016 at the age of 79. I here present a brief obituary with special emphasis on Prof. Trüper’s contributions to our understanding of the halophilic prokaryotes and their adaptations to life in hypersaline environments. He has pioneered the study of the halophilic anoxygenic phototrophic sulfur bacteria of the Ectothiorhodospira—Halorhodospira group. Some of the species he and his group isolated from hypersaline and haloalkaline environments have become model organisms for the study of the mechanisms of haloadaptation: the functions of three major organic compounds – glycine betaine, ectoine, and trehalose – known to serve as “compatible solutes” in halophilic members of the Bacteria domain, were discovered during studies of these anoxygenic phototrophs. Prof. Trüper’s studies of hypersaline alkaline environments in Egypt also led to the isolation of the first known extremely halophilic archaeon (Natronomonas pharaonis. The guest editors dedicate this special volume of Life to the memory of Prof. Hans Georg Trüper.

  7. “shoWing resPecT” in BiBLe TrAnsLATion

    African Journals Online (AJOL)

    ABsTrAcT. This article focuses on the way respect is shown by using 2nd person personal pronouns in languages that distinguish between you plural (you all), you honorific (polite) and you singular (familiar) forms. it discusses the likely influence of the well known rus sian synodal Translation on other translations in the ...

  8. TR146 cells grown on filters as a model of human buccal epithelium

    DEFF Research Database (Denmark)

    Nielsen, Hanne Mørck; Verhoef, J C; Ponec, M

    1999-01-01

    The aim of the present study was to characterize the TR146 cell culture model as an in vitro model of human buccal epithelium with respect to the permeability of test substances with different molecular weights (M(w)). For this purpose, the apparent permeability (P(app)) values for mannitol...

  9. Rezension: Träume von Räumen von Georges Perec

    Directory of Open Access Journals (Sweden)

    Thomas Ballhausen

    2015-12-01

    Full Text Available Mit "Träume von Räumen" liegt ein zentraler Text aus dem umfangreichen Œuvre Geroges Perecs vor, der zuletzt 1990 als Übersetzung greifbar war. Der ursprünglich 1974 erschienene Band ist in mehrfacher Hinsicht wesentlich für das Verständnis Perecs und seiner literarisch-philosophische Wunderwelt ...

  10. Codon Optimization Leads to Functional Impairment of RD114-TR Envelope Glycoprotein.

    Science.gov (United States)

    Zucchelli, Eleonora; Pema, Monika; Stornaiuolo, Anna; Piovan, Claudia; Scavullo, Cinzia; Giuliani, Erica; Bossi, Sergio; Corna, Stefano; Asperti, Claudia; Bordignon, Claudio; Rizzardi, Gian-Paolo; Bovolenta, Chiara

    2017-03-17

    Lentiviral vectors (LVs) are a highly valuable tool for gene transfer currently exploited in basic, applied, and clinical studies. Their optimization is therefore very important for the field of vectorology and gene therapy. A key molecule for LV function is the envelope because it guides cell entry. The most commonly used in transiently produced LVs is the vesicular stomatitis virus glycoprotein (VSV-G) envelope, whose continuous expression is, however, toxic for stable LV producer cells. In contrast, the feline endogenous retroviral RD114-TR envelope is suitable for stable LV manufacturing, being well tolerated by producer cells under constitutive expression. We have previously reported successful, transient and stable production of LVs pseudotyped with RD114-TR for good transduction of T lymphocytes and CD34+ cells. To further improve RD114-TR-pseudotyped LV cell entry by increasing envelope expression, we codon-optimized the RD114-TR open reading frame (ORF). Here we show that, despite the RD114-TRco precursor being produced at a higher level than the wild-type counterpart, it is unexpectedly not duly glycosylated, exported to the cytosol, and processed. Correct cleavage of the precursor in the functional surface and transmembrane subunits is prevented in vivo, and, consequently, the unprocessed precursor is incorporated into LVs, making them inactive.

  11. Comparing Diagnostic Outcomes of Autism Spectrum Disorder Using "DSM-IV-TR" and "DSM-5" Criteria

    Science.gov (United States)

    Harstad, Elizabeth B.; Fogler, Jason; Sideridis, Georgios; Weas, Sarah; Mauras, Carrie; Barbaresi, William J.

    2015-01-01

    Controversy exists regarding the "DSM-5" criteria for ASD. This study tested the psychometric properties of the "DSM-5" model and determined how well it performed across different gender, IQ, and "DSM-IV-TR" sub-type, using clinically collected data on 227 subjects (median age = 3.95 years, majority had IQ > 70).…

  12. Pseudosubstrate regulation of the SCF(beta-TrCP) ubiquitin ligase by hnRNP-U

    DEFF Research Database (Denmark)

    Davis, Matti; Hatzubai, Ada; Andersen, Jens S

    2002-01-01

    beta-TrCP/E3RS (E3RS) is the F-box protein that functions as the receptor subunit of the SCF(beta-TrCP) ubiquitin ligase (E3). Surprisingly, although its two recognized substrates, IkappaB(alpha) and beta-catenin, are present in the cytoplasm, we have found that E3RS is located predominantly...... by competition with a pIkappaB(alpha) peptide, or by a specific point mutation in the E3RS WD region, indicating an E3-substrate-type interaction. However, unlike pI(kappa)Balpha, which is targeted by SCF(beta-TrCP) for degradation, the E3-bound hnRNP-U is stable and is, therefore, a pseudosubstrate....... Consequently, hnRNP-U engages a highly neddylated active SCF(beta-TrCP), which dissociates in the presence of a high-affinity substrate, resulting in ubiquitination of the latter. Our study points to a novel regulatory mechanism, which secures the localization, stability, substrate binding threshold...

  13. Energetska tržišta na početku druge dekade XXI. stoljeća

    OpenAIRE

    Dekanić, Igor

    2011-01-01

    U radu se analiziraju prilike na energetskim tržištima početkom druge dekade XXI. stoljeća kao posljedica razvitka tržišta, globalizacije svih tržišta, a u prvom redu energetskih tržišta, kao i utjecaj geopolitike na energiju. Povećanje cijena energije izazvano je prije svega porastom potrošnje, do kojeg je došlo na valu industrijalizacije zemalja u Aziji i Južnoj Americi, u prvom redu Kine, Indije i Brazila, kao i povremenim geopolitičkim nestabilnostima, koje u energetici redovito izazivaju...

  14. As “Geometrias" do Tráfico: o Comércio Metropolitano e o Tráfico de Escravos em Angola (1796-1807

    Directory of Open Access Journals (Sweden)

    Maximiliano M. Menz

    2012-06-01

    Full Text Available O artigo representa uma análise comparada dos registros alfandegários de Portugal e Angola entre 1796 e 1807 e demonstra que a metrópole portuguesa era responsável por mais da metade do financiamento do tráfico em Angola neste período. Além disso, discute aspectos relacionados ao financiamento do resgate e do transporte de escravos, reavaliando a participação dos mercados coloniais no negócio.

  15. Assessment of lnternational Space Station (ISS) Lithium-ion Battery Thermal Runaway (TR)

    Science.gov (United States)

    Graika, Jason

    2017-01-01

    This task was developed in the wake of the Boeing 787 Dreamliner lithium-ion battery TR incidents of January 2013 and January 2014. The Electrical Power Technical Discipline Team supported the Dreamliner investigations and has followed up by applying lessons learned to conduct an introspective evaluation of NASA's risk of similar incidents in its own lithium-ion battery deployments. This activity has demonstrated that historically NASA, like Boeing and others in the aerospace industry, has emphasized the prevention of TR in a single cell within the battery (e.g., cell screening) but has not considered TR severity-reducing measures in the event of a single-cell TR event. center dotIn the recent update of the battery safety standard (JSC 20793) to address this paradigm shift, the NASA community included requirements for assessing TR severity and identifying simple, low-cost severity reduction measures. This task will serve as a pathfinder for meeting those requirements and will specifically look at a number of different lithium-ion batteries currently in the design pipeline within the ISS Program batteries that, should they fail in a Dreamliner-like incident, could result in catastrophic consequences. This test is an abuse test to understand the heat transfer properties of the cell and ORU in thermal runaway, with radiant barriers in place in a flight like test in on orbit conditions. This includes studying the heat flow and distribution in the ORU. This data will be used to validate the thermal runaway analysis. This test does not cover the ambient pressure case. center dotThere is no pass/ fail criteria for this test.

  16. Continuously Tunable Ca2+ Regulation of RNA-Edited CaV1.3 Channels

    Directory of Open Access Journals (Sweden)

    Hojjat Bazzazi

    2013-10-01

    Full Text Available CaV1.3 ion channels are dominant Ca2+ portals into pacemaking neurons, residing at the epicenter of brain rhythmicity and neurodegeneration. Negative Ca2+ feedback regulation of CaV1.3 channels (CDI is therefore critical for Ca2+ homeostasis. Intriguingly, nearly half the CaV1.3 transcripts in the brain are RNA edited to reduce CDI and influence oscillatory activity. It is then mechanistically remarkable that this editing occurs precisely within an IQ domain, whose interaction with Ca2+-bound calmodulin (Ca2+/CaM is believed to induce CDI. Here, we sought the mechanism underlying the altered CDI of edited channels. Unexpectedly, editing failed to attenuate Ca2+/CaM binding. Instead, editing weakened the prebinding of Ca2+-free CaM (apoCaM to channels, which proves essential for CDI. Thus, editing might render CDI continuously tunable by fluctuations in ambient CaM, a prominent effect we substantiate in substantia nigral neurons. This adjustability of Ca2+ regulation by CaM now looms as a key element of CNS Ca2+ homeostasis.

  17. Imaging Ca2+ with a Fluorescent Rhodol.

    Science.gov (United States)

    Contractor, Alisha A; Miller, Evan W

    2018-01-16

    Ca2+ mediates a host of biochemical and biophysical signaling processes in cells. The development of synthetic, Ca2+-sensitive fluorophores has played an instrumental role in our understanding of the temporal and spatial dynamics of Ca2+. Coupling Ca2+-selective ligands to fluorescent reporters has provided a wealth of excellent indicators that span the visible excitation and emission spectrum and possess Ca2+ affinities suited to a variety of cellular contexts. One underdeveloped area is the use of hybrid rhodamine/fluorescein fluorophores, or rhodols, in the context of Ca2+ sensing. Rhodols are bright and photostable and have good two-photon absorption cross sections (σTPA), making them excellent candidates for incorporation into Ca2+-sensing scaffolds. Here, we present the design, synthesis, and application of rhodol Ca2+ sensor 1 (RCS-1), a chlorinated pyrrolidine-based rhodol. RCS-1 possesses a Ca2+ binding constant of 240 nM and a 10-fold turn response to Ca2+. RCS-1 effectively absorbs infrared light and has a σTPA of 76 GM at 840 nm, 3-fold greater than that of its fluorescein-based counterpart. The acetoxy-methyl ester of RCS-1 stains the cytosol of live cells, enabling observation of Ca2+ fluctuations and cultured neurons using both one- and two-photon illumination. Together, these results demonstrate the utility of rhodol-based scaffolds for Ca2+ sensing using two-photon illumination in neurons.

  18. Ca2+ Dependence of Synaptic Vesicle Endocytosis.

    Science.gov (United States)

    Leitz, Jeremy; Kavalali, Ege T

    2016-10-01

    Ca(2+)-dependent synaptic vesicle recycling is essential for structural homeostasis of synapses and maintenance of neurotransmission. Although, the executive role of intrasynaptic Ca(2+) transients in synaptic vesicle exocytosis is well established, identifying the exact role of Ca(2+) in endocytosis has been difficult. In some studies, Ca(2+) has been suggested as an essential trigger required to initiate synaptic vesicle retrieval, whereas others manipulating synaptic Ca(2+) concentrations reported a modulatory role for Ca(2+) leading to inhibition or acceleration of endocytosis. Molecular studies of synaptic vesicle endocytosis, on the other hand, have consistently focused on the roles of Ca(2+)-calmodulin dependent phosphatase calcineurin and synaptic vesicle protein synaptotagmin as potential Ca(2+) sensors for endocytosis. Most studies probing the role of Ca(2+) in endocytosis have relied on measurements of synaptic vesicle retrieval after strong stimulation. Strong stimulation paradigms elicit fusion and retrieval of multiple synaptic vesicles and therefore can be affected by several factors besides the kinetics and duration of Ca(2+) signals that include the number of exocytosed vesicles and accumulation of released neurotransmitters thus altering fusion and retrieval processes indirectly via retrograde signaling. Studies monitoring single synaptic vesicle endocytosis may help resolve this conundrum as in these settings the impact of Ca(2+) on synaptic fusion probability can be uncoupled from its putative role on synaptic vesicle retrieval. Future experiments using these single vesicle approaches will help dissect the specific role(s) of Ca(2+) and its sensors in synaptic vesicle endocytosis. © The Author(s) 2015.

  19. An Inhibitory Effect of Extracellular Ca2+ on Ca2+-Dependent Exocytosis

    Science.gov (United States)

    Wang, Yeshi; Chen, Xiaowei; Sun, Lei; Guo, Ning; Zheng, Hui; Zheng, Lianghong; Ruat, Martial; Han, Weiping; Zhang, Claire Xi; Zhou, Zhuan

    2011-01-01

    Aim Neurotransmitter release is elicited by an elevation of intracellular Ca2+ concentration ([Ca2+]i). The action potential triggers Ca2+ influx through Ca2+ channels which causes local changes of [Ca2+]i for vesicle release. However, any direct role of extracellular Ca2+ (besides Ca2+ influx) on Ca2+-dependent exocytosis remains elusive. Here we set out to investigate this possibility on rat dorsal root ganglion (DRG) neurons and chromaffin cells, widely used models for studying vesicle exocytosis. Results Using photolysis of caged Ca2+ and caffeine-induced release of stored Ca2+, we found that extracellular Ca2+ inhibited exocytosis following moderate [Ca2+]i rises (2–3 µM). The IC50 for extracellular Ca2+ inhibition of exocytosis (ECIE) was 1.38 mM and a physiological reduction (∼30%) of extracellular Ca2+ concentration ([Ca2+]o) significantly increased the evoked exocytosis. At the single vesicle level, quantal size and release frequency were also altered by physiological [Ca2+]o. The calcimimetics Mg2+, Cd2+, G418, and neomycin all inhibited exocytosis. The extracellular Ca2+-sensing receptor (CaSR) was not involved because specific drugs and knockdown of CaSR in DRG neurons did not affect ECIE. Conclusion/Significance As an extension of the classic Ca2+ hypothesis of synaptic release, physiological levels of extracellular Ca2+ play dual roles in evoked exocytosis by providing a source of Ca2+ influx, and by directly regulating quantal size and release probability in neuronal cells. PMID:22028769

  20. Puente Cañas

    OpenAIRE

    Luis Sáenz

    2017-01-01

    En la carretera de Trinidad a Cienfuegos, provincia de Las Villas (Cuba), se ha construido un puente isostático sobre el río Cañas, de hormigón pretensado, de tres tramos, cuya sección transversal es celular de tres tabiques formando un doble cajón. Los dos tramos laterales o accesos al central, de 15,5 m de luz cada uno, se han construido en voladizo respecto a los dos apoyos centrales. El tramo central salva un vano de 76 m.

  1. Puente Cañas

    Directory of Open Access Journals (Sweden)

    Luis Sáenz

    2017-05-01

    Full Text Available En la carretera de Trinidad a Cienfuegos, provincia de Las Villas (Cuba, se ha construido un puente isostático sobre el río Cañas, de hormigón pretensado, de tres tramos, cuya sección transversal es celular de tres tabiques formando un doble cajón. Los dos tramos laterales o accesos al central, de 15,5 m de luz cada uno, se han construido en voladizo respecto a los dos apoyos centrales. El tramo central salva un vano de 76 m.

  2. A inclusão de surdos no trânsito

    Directory of Open Access Journals (Sweden)

    Vânia Mendes de Souza

    Full Text Available RESUMO: Objetivo: investigar como se dá o processo de inclusão de surdos no cenário do trânsito. Métodos: participaram do estudo 20 sujeitos, divididos em três grupos: G1, composto por 10 surdos; G2 composto por cinco profissionais de uma instituição de referência em assistência a sujeitos com necessidades educacionais específicas e G3 composto por cinco profissionais de um Departamento Estadual de Trânsito. Foram aplicadas entrevistas gravadas em áudio e vídeo, cujas respostas foram analisadas por meio do software Qualiquantisoft e aquelas com sentido semelhante foram reunidas para a construção de discursos do sujeito coletivo, tendo como base o método proposto por Lefèvre e Lefèvre. Resultados: os discursos do sujeito coletivo apontam para dificuldades na acessibilidade de surdos no trânsito e no processo de obtenção e renovação da carteira nacional de habilitação. De uma forma geral, todos os grupos apontaram sugestões como: a necessidade de intérprete para mediar o processo, a capacitação de profissionais para atenderem surdos e a realização da prova teórica em Libras, utilizando recursos de vídeo. Conclusão: embora haja avanços, há inúmeras falhas no que se trata da acessibilidade de pessoas surdas tanto no processo de obtenção/renovação da carteira nacional de habilitação quanto no trânsito. Portanto, é urgente que seja discutida a realidade enfrentada por essa população, visando soluções que possam garantir aos surdos não apenas o acesso ao trânsito, mas também a construção e adequada execução de políticas públicas que venham a lhes fornecer acessibilidade e segurança.

  3. Transport and Fatigue Properties of Ferroelectric Polymer P(VDF-TrFE) For Nonvolatile Memory Applications

    KAUST Repository

    Hanna, Amir

    2012-06-01

    Organic ferroelectrics polymers have recently received much interest for use in nonvolatile memory devices. The ferroelectric copolymer poly(vinylidene fluoride- trifluoroethylene) , P(VDF-TrFE), is a promising candidate due to its relatively high remnant polarization, low coercive field, fast switching times, easy processability, and low Curie transition. However, no detailed study of charge injection and current transport properties in P(VDF-TrFE) have been reported in the literature yet. Charge injection and transport are believed to affect various properties of ferroelectric films such as remnant polarization values and polarization fatigue behavior.. Thus, this thesis aims to study charge injection in P(VDF-TrFE) and its transport properties as a function of electrode material. Injection was studied for Al, Ag, Au and Pt electrodes. Higher work function metals such as Pt have shown less leakage current compared to lower work function metals such as Al for more than an order of magnitude. That implied n-type conduction behavior for P(VDF-TrFE), as well as electrons being the dominant injected carrier type. Charge transport was also studied as a function of temperature, and two major transport regimes were identified: 1) Thermionic emission over a Schottky barrier for low fields (E < 25 MV/m). 2) Space-Charge-Limited regime at higher fields (25 < E <120 MV/m). We have also studied the optical imprint phenomenon, the polarization fatigue resulting from a combination of broad band optical illumination and DC bias near the switching field. A setup was designed for the experiment, and validated by reproducing the reported effect in polycrystalline Pb(Zr,Ti)O3 , PZT, film. On the other hand, P(VDF-TrFE) film showed no polarization fatigue as a result of optical imprint test, which could be attributed to the large band gap of the material, and the low intensity of the UV portion of the arc lamp white light used for the experiment. Results suggest using high work

  4. Trænings- og habiliteringstilbud til børn og unge med handicap - et vidensoverblik

    DEFF Research Database (Denmark)

    Wendt, Rikke Eline; Jørgensen, Anne-Marie Klint

    2015-01-01

    Der er i dag ikke et aktuelt billede af gode trænings- og habiliteringstilbud, som iværksættes i og udenfor de specialiserede tilbud til børn og unge med betydelige funktionsnedsættelser. Foreliggende projekt tilbyder et vidensoverblik over ikke-hjemmebaserede trænings og habiliteringsindsatser, ...

  5. Atención de Enfermería al adulto en accidentes de tráfico

    OpenAIRE

    García Méndez, Beatriz

    2015-01-01

    Realizar una revisión de los cuidados de enfermería que deben ser conocidas por los profesionales a la hora de atender un accidente de tráfico. - Explicar los conceptos fundamentales sobre accidentes de tráfico. - Revisar artículos y bases de datos relacionados con el tema.

  6. Autism Spectrum Disorders According to "DSM-IV-TR" and Comparison with "DSM-5" Draft Criteria: An Epidemiological Study

    Science.gov (United States)

    Mattila, Marja-Leena; Kielinen, Marko; Linna, Sirkka-Liisa; Jussila, Katja; Ebeling, Hanna; Bloigu, Risto; Joseph, Robert M.; Moilanen, Irma

    2011-01-01

    Objective: The latest definitions of autism spectrum disorders (ASDs) were specified in "DSM-IV-TR" in 2000. "DSM-5" criteria are planned for 2013. Here, we estimated the prevalence of ASDs and autism according to "DSM-IV-TR," clarified confusion concerning diagnostic criteria, and evaluated "DSM-5" draft…

  7. La mise en patrimoine sur ONF.ca

    Directory of Open Access Journals (Sweden)

    Michèle Garneau

    2015-05-01

    Full Text Available Résumé Ce texte propose une approche à la fois descriptive, analytique et critique de la très active production patrimoniale actuellement en cours sur ONF.ca. En se donnant comme mission de rendre accessible son patrimoine audiovisuel, l’Office se soucie d’en orienter la réception, de proposer aux internautes l’histoire de son action audiovisuelle depuis sa fondation en 1939. Par le relevé d’un certain nombre de valeurs exhibées (authenticité, représentativité, renommée et proximité, et de registres privilégiés (dont celui de la commémoration, l’auteur tente de circonscrire une axiologie du patrimoine sur ONF.ca. Dans sa seconde partie, le texte se consacre à la question de l’articulation (et de l’écart entre mémoire patrimoniale et mémoires particulières (des films afin de mieux cerner les contours politiques et les enjeux idéologiques de l’encadrement discursif des collections. Abstract This article offers a descriptive, analytical and critical approach to the very active production of audiovisual heritage currently underway at NFB.ca. Designed to make the National Film Board (NFB’s audiovisual heritage more accessible, the website carefully guides its users through the history-memory of audiovisual production at the Film Board from its foundation in 1939 through to the present. By analyzing how the website showcases its films, endorsing specific values (authenticity, representativeness, recognition and proximity and privileging certain registers (such as commemoration, the article first attempts to define an axiology of heritage in NFB.ca. The article then focuses on questions of the articulation of (and the variation between history-memory and particular memories (of films in order to better outline the political contours and ideological stakes of the discursive framework of the NFB’s collections.

  8. Diagnósticos de enfermagem em pós-operatório imediato de cirurgia bariátríca em terapia intensiva

    OpenAIRE

    Santos, Bruna Nogueira dos [UNESP; Castro, Meire Cristina Novelli e [UNESP; Fontes, Cassiana Mendes Bertoncello [UNESP; Dell'Acqua, Magda Cristina Queiroz [UNESP

    2015-01-01

    Objective: characterizing the clinical profile of patients in post-operatory of bariatric surgery in intensive care and describing the nursing diagnoses. Method: a descriptive, cross-sectional and prospective study with 13 patients in post-operatory of bariatric surgery in public and teaching hospitals. Through the history of Nursing and Nursing Diagnoses were formulated according to the NANDA Taxonomy II from June to August 2010. Quantitative data analysis was performed. The research project...

  9. Multiple Ca2+ sensors in secretion

    DEFF Research Database (Denmark)

    Walter, Alexander M; Groffen, Alexander J; Sørensen, Jakob Balslev

    2011-01-01

    Regulated neurotransmitter secretion depends on Ca(2+) sensors, C2 domain proteins that associate with phospholipids and soluble N-ethylmaleimide-sensitive fusion attachment protein receptor (SNARE) complexes to trigger release upon Ca(2+) binding. Ca(2+) sensors are thought to prevent spontaneous...... fusion at rest (clamping) and to promote fusion upon Ca(2+) activation. At least eight, often coexpressed, Ca(2+) sensors have been identified in mammals. Accumulating evidence suggests that multiple Ca(2+) sensors interact, rather than work autonomously, to produce the complex secretory response...... observed in neurons and secretory cells. In this review, we present several working models to describe how different sensors might be arranged to mediate synchronous, asynchronous and spontaneous neurotransmitter release. We discuss the scenario that different Ca(2+) sensors typically act on one shared...

  10. Porto de Trás: etnicidade, turismo e patrimonialização

    Directory of Open Access Journals (Sweden)

    Patrícia de Araújo Brandão Couto

    2011-05-01

    Full Text Available The article describes the process by which cultural heritage of Porto de Trás, a neigborhood in Itacaré on the southern coast of Bahia, Brazil, has gained recognition. Porto de Trás is an ethnic community of African Brazilians with a tradition of artisanal fishing. Historically, its residents, organized in family groupings, have been segregated for their race and socioeconomic status.Even with the arrival of the tourist industry in the 1990s and concomitant urban developments, it maintained its traditional cultural practices, gaining recognition as an enclave of “authenticity” referred to local culture. Interactions between the different spheres of heritage operating in this space are discussed especially the construction of ethnicity by local residents.

  11. En rød tråd i forfatterskabet

    DEFF Research Database (Denmark)

    Toft, Herdis

    2003-01-01

    Med fokus på stilistiske og tematiske træk i Snøvsen-bøger og -film gennemgås Benny Andersens produktion for børn. Om forfatterens forhold til sprogets, fantasiens, rolleudførelsens og rollefordelingens underfundige spilleregler. Og om naivitetens særlige form for livsklogskab.......Med fokus på stilistiske og tematiske træk i Snøvsen-bøger og -film gennemgås Benny Andersens produktion for børn. Om forfatterens forhold til sprogets, fantasiens, rolleudførelsens og rollefordelingens underfundige spilleregler. Og om naivitetens særlige form for livsklogskab....

  12. AN EFFICIENT DATA MINING METHOD TO FIND FREQUENT ITEM SETS IN LARGE DATABASE USING TR- FCTM

    Directory of Open Access Journals (Sweden)

    Saravanan Suba

    2016-01-01

    Full Text Available Mining association rules in large database is one of most popular data mining techniques for business decision makers. Discovering frequent item set is the core process in association rule mining. Numerous algorithms are available in the literature to find frequent patterns. Apriori and FP-tree are the most common methods for finding frequent items. Apriori finds significant frequent items using candidate generation with more number of data base scans. FP-tree uses two database scans to find significant frequent items without using candidate generation. This proposed TR-FCTM (Transaction Reduction- Frequency Count Table Method discovers significant frequent items by generating full candidates once to form frequency count table with one database scan. Experimental results of TR-FCTM shows that this algorithm outperforms than Apriori and FP-tree.

  13. Tráfico internacional de mulheres: violência e representações cotidianas

    OpenAIRE

    Peixoto, Maria Angélica

    2016-01-01

    O tráfico de pessoas aparece, contemporaneamente, como um dos mais graves problemas sociais de âmbito internacional. Ele é inseparável da violência e, por isso, a análise desse fenômeno social é muito importante, pois a sociedade vive em um momento contraditório no qual avanços convivem com retrocessos, em que há o aumento da violência e da percepção de sua existência. Há um crescimento do tráfico de pessoas e esse fenômeno atinge certos setores da população, especialmente as m...

  14. Predlog načrta trženja za Mladinski hotel in Hotel Bistra

    OpenAIRE

    Markovič, Barbara

    2016-01-01

    Za manjše kraje, kot je Vrhnika, je turizem ena izmed razvojnih priložnosti za večjo prepoznavnost med ponudniki. Mladinski hotel in Hotel Bistra, ki je odprl svoja vrata v drugi polovici leta 2014 in se trži tudi pod imenom Gostišče s prenočitvami. Zaradi hitre menjave lastništva nastanitveni objekt še vedno nima načrta trženja, ki predstavlja sicer ključen sestavni del razvojnega in operativnega načrta delujočega podjetja. Brez prave strategije in skrbno določenih ciljev podjetje nima zagot...

  15. Factores tróficos en neuronas dopaminérgicas mesencefálicas

    Directory of Open Access Journals (Sweden)

    G. Pinzón

    1994-12-01

    Full Text Available Desde su descubrimiento, se ha postulado, y en algunos casos comprobado, que los factores tróficos juegan un papel importante en el desarrollo, mantenimiento y regeneración del sistema nervioso. El conocimiento y la manipulación de estas sustancias ha servido para: a formular nuevas teorías acerca de la etiología de varios desórdenes neurodebeneraivos y b desarrollar nuevas altemativas terapéuticas para estas entidades. En la presente revisión, resumimos los principales efectos de diferentes factores tróficos sobre las neuronas dopaminérgicas de la substantia nigra en desarrollo, cuya degeneración en el adulto ocasiona la mayoría de los síntomas observados en la enfermedad de Parkinson.

  16. A TR-induced algorithm for hot spots elimination through CT-scan HIFU simulations

    Science.gov (United States)

    Leduc, Nicolas; Okita, Kohei; Sugiyama, Kazuyasu; Takagi, Shu; Matsumoto, Yoichiro

    2011-09-01

    Although nowadays widely spread for imaging and treatments uses, HIFU techniques are still limited by the distortion of the wavefront due to refraction and reflection on the inhomogeneous media inside the human body. CT-scan Time Reversal (TR) procedure has risen as a promising candidate for focus control. A finite difference time domain parallelized code is used to provide simulations of TR-enhanced propagation through elements of the human body and implement a simple algorithm to address the issue of grating lobes, i.e secondary peaks of pressure due to natural diffraction by phased arrays and enhanced by medium heterogeneity. Using an iterative, progressive process combining secondary sound sources and independent signal summation, the primary peak is strengthened while secondary peaks are increasingly obliterated. This method supports the feasibility of precise modification and enhancement of the pressure profile in the targeted area through Time Reversal based solutions.

  17. A case of pancreatic cancer with the sign of Leser-Trélat.

    Science.gov (United States)

    Hirano, T; Yoshioka, H; Manabe, T

    1993-07-01

    A case of 79-year-old man with pancreatic cancer associated with the sign of Leser-Trélat was presented. Abdominal CT scanning was performed and a pancreatic tail tumor involving the spleen and the greater omentum as well as ascitic retention were detected. Ba-enema study showed the stenosis of the sigmoid colon, suggesting the peritoneal disseminations. Absolute non-curative operation of side-to-side transverso-sigmoidostomy was performed for colonic stenosis, and biopsy of the disseminated omental tumor was performed, which was histologically diagnosed as well differentiated papillary adenocarcinoma compatible with pancreatic cancer. The postoperative course was uneventful and at present the patient was followed at outpatient clinic. To our knowledge, this case is the first report with pancreatic cancer associated with the sign of Leser-Trélat in Japan.

  18. Sensitivity of diamond-capped impedance transducer to Tröger’s base derivatives

    Czech Academy of Sciences Publication Activity Database

    Stehlík, Štěpán; Ižák, Tibor; Kromka, Alexander; Dolenský, B.; Havlík, M.; Rezek, Bohuslav

    2012-01-01

    Roč. 4, č. 8 (2012), s. 3860-3865 ISSN 1944-8244 R&D Projects: GA ČR(CZ) GBP108/12/G108 Institutional research plan: CEZ:AV0Z10100521 Keywords : Tröger’s base derivative * nanocrystalline diamond * chemical sensor * impedance spectroscopy * surface conductivity Subject RIV: BM - Solid Matter Physics ; Magnetism Impact factor: 5.008, year: 2012

  19. Um Problema de Três Corpos Analiticamente Solúvel

    Directory of Open Access Journals (Sweden)

    Figuerêdo Elysandra

    2001-01-01

    Full Text Available Analisamos um problema de três corpos interagindo mutuamente via forças harmônicas no contexto do formalismo newtoniano. Uma solução analítica exata para este problema é encontrada por meio de uma abordagem didática e os caminhos para a análise do problema de N corpos são indicados.

  20. Discordance gradient-surface dans le rétrécissement mitral: le ...

    African Journals Online (AJOL)

    Il s'agit d'une étude transversale monocentrique incluant tous les patients admis au service de Cardiologie du CHU Ibn Rochd de Casablanca pour un RM serré ou très serré, sur une période d'une année (Janvier 2014 à Décembre 2014). Nous avons analysés séparément deux groupes de patients : ceux avec un gradient ...

  1. El tránsito vehículo-peatonal desde un punto de vista psicocultural

    Directory of Open Access Journals (Sweden)

    Gilbert Ulloa Brenes

    2011-12-01

    Full Text Available El objetivo de este artículo es proponer una serie de insumos epistemológicos y teóricos, como aproximación psicocultural, desde la cual se entienda el tránsito de vehículos y peatones en calidad de campo cultural propio de la sociedad moderna industrializada. Dicha propuesta sintetiza parte de los resultados de una investigación básica previa. La idea central de este trabajo supone que, en el tránsito vehículo-peatonal, se expresan aspectos como el ideal del maquinismo, algunos elementos de la ideología capitalista y una forma particular de constitución de los sujetos que participan en él. La construcción teórica se basa en las tesis de Max Horkheimer sobre las determinaciones sociales en procesos como la percepción y la formación de conceptos, las formulaciones de Pierre Bourdieu respecto a la constitución de los campos sociales y la perspectiva de Jacques Lacan en torno a lo simbólico y la identificación. A partir de esta propuesta, se concluye que una perspectiva psicocultural del tránsito de vehículos y peatones se basaría en la indagación sobre la racionalidad y la ideología imperante en los fenómenos asociados al tránsito vehículo peatonal, en la consideración de la estructura particular de este campo y en la forma como lo simbólico ubica a los sujetos participantes en él.

  2. Trådbindere til forankring af skalmure og hule mure

    DEFF Research Database (Denmark)

    Høffding Knutsson, H.

    Anvisningen er et hjælpemiddel for projekterende, udførende og kontrollerende parter ved dimensionering, montering og vurdering af trådbindere. Den indeholder bæreevnediagrammer for de gængse størrelser bindere i tinbronze og rustfri stål, både bæreevnen af retlinede bindere, men også den...

  3. Klippel-Trénaunay Syndrome with Intracranial Arteriovenous Malformation: A Rare Presentation

    Directory of Open Access Journals (Sweden)

    Mahniya F. Sadiq

    2014-01-01

    Full Text Available Klippel-Trénaunay syndrome (KTS is a rare vascular congenital anomaly affecting less than 200,000 people in the United States. Vascular malformations associated with KTS tend to affect slow flow systems: venous, capillary, and lymphatic systems. The nature of the syndrome leads to a higher risk for the development of arteriovenous malformations. Our case presentation describes a patient with KTS and an associated rare presentation of intraventricular arteriovenous malformation (AVM.

  4. ¿Puede la música expresar lo trágico?

    Directory of Open Access Journals (Sweden)

    Enrico Fubini

    2013-12-01

    Full Text Available [ES] Este trabajo parte de la pregunta sobre si el arte musical es capaz de expresar realmente el sentimiento trágico, e intenta clarificar la relación que existe (si existe entre el concepto de lo trágico y el lenguaje de la música. En concreto, se trata de reconstruir la historia de esta relación empezando por sus raíces, hundidas en la tragedia griega, para llegar a sus más cercanas expresiones en el siglo XX. Se pone el acento en el valor trágico del propio contenido musical, destacando con atención, en el caso del melodrama, la relación que surge entre una emoción expresada en palabras y aquella que nace de la música misma, así como la contradicción aparente entre la belleza de la armonía de la música polifónica con el sentimiento de lo verdaderamente trágico. ; [EN] This writing raises the question if the musical art is able to really express the feeling of the tragic, and tries to clarify the relation that exist (if it exists between the concept of the tragic and the language of music. In particular, it aims to reconstruct the history of this relation beginning by its roots, sunk in Greek tragedy, to arrive at its nearer expressions in the XX century. The accent is on the tragic value of the musical content itself, emphasizing in particular, in the case of the melodrama, the relation that arise between an emotion expressed in words and that that is born of music itself, as well as the apparent contradiction between the beauty of the harmony of polyphonic music and the feeling of the truly tragic.

  5. La socialisation sexospécifique des très jeunes adolescents à l ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    Les adolescents de l'Afrique subsaharienne, en particulier ceux issus de zones urbaines à faible revenu, sont très vulnérables en ce qui concerne la santé sexuelle et reproductive. La plupart des interventions visant à améliorer la santé sexuelle et reproductive des adolescents sont axées sur les adolescents plus âgés et ...

  6. Schwannome benin primitif retrovesical : une tumeur très rare à ...

    African Journals Online (AJOL)

    Le schwannome est une tumeur le plus souvent bénigne, d'origine nerveuse se développant à partir des cellules de la gaine de Schwann. C'est une tumeur très rare tant par sa fréquence que par sa localisation rétrovésicale. L'examen anatomopathologique et immunohistochimique confirme le type histologique ainsi que ...

  7. Analyste financier, Trésorerie et opérations comptables (h/f) | CRDI ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    Sous la direction du Comptable trésorerie, l'analyste financier s'occupe des créances, des frais payés d'avance et des engagements opérationnels, rédige les notes afférentes aux états financiers et traite les factures et les demandes de paiement. Pour ce faire, il doit notamment veiller au respect des échéanciers de fin de ...

  8. Immunoglobulin (IG and T cell receptor (TR genes: IMGT® and the birth and rise of immunoinformatics

    Directory of Open Access Journals (Sweden)

    Marie-Paule eLefranc

    2014-02-01

    Full Text Available IMGT®, the international ImMunoGeneTics information system®, http://www.imgt.org (CNRS and Université Montpellier 2 is the global reference in immunogenetics and immunoinformatics. By its creation in 1989, IMGT® marked the advent of immunoinformatics, which emerged at the interface between immunogenetics and bioinformatics. IMGT® is specialized in the immunoglobulins (IG or antibodies, T cell receptors (TR, major histocompatibility (MH, and proteins of the IgSF and MhSF superfamilies. IMGT® has been built on the IMGT-ONTOLOGY axioms and concepts, which bridged the gap between genes, sequences and three-dimensional (3D structures. The concepts include the IMGT® standardized keywords (concepts of identification, IMGT® standardized labels (concepts of description, IMGT® standardized nomenclature (concepts of classification, IMGT unique numbering and IMGT Colliers de Perles (concepts of numerotation. IMGT® comprises 7 databases, 15,000 pages of web resources and 17 tools, and provides a high-quality and integrated system for the analysis of the genomic and expressed IG and TR repertoire of the adaptive immune responses. Tools and databases are used in basic, veterinary and medical research, in clinical applications (mutation analysis in leukemia and lymphoma and in antibody engineering and humanization. They include, as examples: IMGT/V-QUEST and IMGT/JunctionAnalysis for nucleotide sequence analysis and their high-throughput version IMGT/HighV-QUEST for next generation sequencing (500,000 sequences per batch, IMGT/DomainGapAlign for amino acid sequence analysis of IG and TR variable and constant domains and of MH groove domains, IMGT/3Dstructure-DB for 3D structures, contact analysis and paratope/epitope interactions of IG/antigen and TR/peptide-MH complexes and IMGT/mAb-DB interface for therapeutic antibodies and fusion proteins for immunological applications (FPIA.

  9. Non–Ca2+-conducting Ca2+ channels in fish skeletal muscle excitation-contraction coupling

    Science.gov (United States)

    Schredelseker, Johann; Shrivastav, Manisha; Dayal, Anamika; Grabner, Manfred

    2010-01-01

    During skeletal muscle excitation-contraction (EC) coupling, membrane depolarizations activate the sarcolemmal voltage-gated L-type Ca2+ channel (CaV1.1). CaV1.1 in turn triggers opening of the sarcoplasmic Ca2+ release channel (RyR1) via interchannel protein–protein interaction to release Ca2+ for myofibril contraction. Simultaneously to this EC coupling process, a small and slowly activating Ca2+ inward current through CaV1.1 is found in mammalian skeletal myotubes. The role of this Ca2+ influx, which is not immediately required for EC coupling, is still enigmatic. Interestingly, whole-cell patch clamp experiments on freshly dissociated skeletal muscle myotubes from zebrafish larvae revealed the lack of such Ca2+ currents. We identified two distinct isoforms of the pore-forming CaV1.1α1S subunit in zebrafish that are differentially expressed in superficial slow and deep fast musculature. Both do not conduct Ca2+ but merely act as voltage sensors to trigger opening of two likewise tissue-specific isoforms of RyR1. We further show that non-Ca2+ conductivity of both CaV1.1α1S isoforms is a common trait of all higher teleosts. This non-Ca2+ conductivity of CaV1.1 positions teleosts at the most-derived position of an evolutionary trajectory. Though EC coupling in early chordate muscles is activated by the influx of extracellular Ca2+, it evolved toward CaV1.1-RyR1 protein–protein interaction with a relatively small and slow influx of external Ca2+ in tetrapods. Finally, the CaV1.1 Ca2+ influx was completely eliminated in higher teleost fishes. PMID:20212109

  10. Temporal monitoring of intracellular Ca2+ signaling and origins of Ca2+ oscillations

    OpenAIRE

    Webb, Dominic-Luc

    2006-01-01

    This thesis examined parameters influencing stimulated cytoplasmic free Ca2+ concentration ([Ca2+]i) oscillations in hepatocytes and pancreatic beta-cells. Hepatic glucose output is regulated in part by hormones such as vasopressin that act through [Ca 2+]i oscillations. Pulsatile [Ca2+]i in beta-cells parallels insulin secretion and this results in potently controlled blood glucose homeostasis. Employing temporal [Ca2+]i measurements and related biochemical assays, efforts ...

  11. Mortalidad por accidentes de tránsito en Bayamo, Cuba 2011

    Directory of Open Access Journals (Sweden)

    Arlines Piña-Tornés

    Full Text Available Con el objetivo de describir la mortalidad por accidentes de tránsito en Bayamo, Cuba, en el año 2011 se realizó una revisión de los pacientes lesionados y fallecidos a causa de accidentes de tránsito, registrados en Hospital Carlos M. de Céspedes. Se atendieron en emergencias 1365 lesionados, predominando el grupo etario de 25 a 44 años con 372 pacientes (27,3%, y el sexo masculino con 1071 (78,5%. Fallecieron 46 personas, en su mayoría del mismo grupo de edad y de sexo masculino. Los traumatismos múltiples (52,6% y cráneofaciales (34,2% fueron las localizaciones predominantes. Se destacaron los atropellos por vehículo de motor con mortalidad del 26,3%. En conclusión, la mortalidad por accidentes de tránsito predomina en adultos jóvenes masculinos; cuyas consecuencias fatales son debido a traumatismos múltiples por atropellos.

  12. Enhanced pyroelectric properties of PZT/PVDF-TrFE composites using calcined PZT ceramic powders

    Science.gov (United States)

    Wu, Chuan-Gui; Cai, Guang-Qiang; Luo, Wen-Bo; Peng, Qiang-Xiang; Sun, Xiang-Yu; Zhang, Wan-Li

    2013-04-01

    The effects of calcined lead-zirconate-titanate (PZT) powders on the electric properties of PZT/polyvinylidene-trifluorethylene copolymer (PVDF-TrFE) composites thick films were studied in this paper. Firstly, the PZT powders synthesized by hydrothermal method were calcined at different temperatures ranging from 300°C to 900°C, and then the PZT/PVDF-TrFE composites films were produced by casting PZT/PVDF-TrFE suspension onto the indium-tin-oxide (ITO)-coated glass substrates. Electric properties, including dielectric and pyroelectric performances of thick films consisting of PZT powders calcined at different temperatures were tested. The highest pyroelectric coefficient obtained in the sample using 700°C calcined PZT powders was 96 μCm-2K-1, which was 20% higher than the composites made of uncalcined powders. Additionally, the highest detectivity figure-of-merit (FOM) (FD) of the composite was 1.36 × 10-5Pa-1/2, which increased about 13.5% compared to the one using uncalcined powders.

  13. Mortes no trânsito do Rio de Janeiro, Brasil

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    Carlos Henrique Klein

    Full Text Available A evolução urbana do município do Rio de Janeiro durante o século XX, especialmente a partir da sua segunda metade, é fortemente marcada por crescentes privilégios concedidos ao crescimento da utilização dos meios de transporte de massa e, principalmente, individuais, feitos por veículos a motor de explosão. Uma das conseqüências desta política é a ascenção da mortalidade por acidentes de trânsito, verificada durante a década de 80, entre homens e mulheres de todas as idades. Neste trabalho demonstra-se também que, em 1990, apenas cerca de 1/3 das vítimas fatais nos acidentes de trânsito estavam "embarcadas" nos veículos. Portanto, a maioria dos óbitos por este tipo de acidente, cerca de 2/3, ocorreu por atropelamentos. Isto indica a necessidade de o poder público reverter a prioridade na prevenção das mortes por acidentes de trânsito em favor de medidas eficazes que protejam os pedestres.

  14. Mortes no trânsito do Rio de Janeiro, Brasil

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    Klein Carlos Henrique

    1994-01-01

    Full Text Available A evolução urbana do município do Rio de Janeiro durante o século XX, especialmente a partir da sua segunda metade, é fortemente marcada por crescentes privilégios concedidos ao crescimento da utilização dos meios de transporte de massa e, principalmente, individuais, feitos por veículos a motor de explosão. Uma das conseqüências desta política é a ascenção da mortalidade por acidentes de trânsito, verificada durante a década de 80, entre homens e mulheres de todas as idades. Neste trabalho demonstra-se também que, em 1990, apenas cerca de 1/3 das vítimas fatais nos acidentes de trânsito estavam "embarcadas" nos veículos. Portanto, a maioria dos óbitos por este tipo de acidente, cerca de 2/3, ocorreu por atropelamentos. Isto indica a necessidade de o poder público reverter a prioridade na prevenção das mortes por acidentes de trânsito em favor de medidas eficazes que protejam os pedestres.

  15. Composites PVDF-TrFE/BT used as bioactive membranes for enhancing bone regeneration

    Science.gov (United States)

    Gimenes, Rossano; Zaghete, Maria A.; Bertolini, Marcio; Varela, Jose A.; Coelho, Luciane O.; Silva, Nelson F., Jr.

    2004-07-01

    In this paper a piezoelectric composite membranes were developed for charge generator to promoter bone regeneration on defects sites. Is known that the osteogenesis process is induced by interactions between biological mechanisms and electrical phenomena. The membranes were prepared by mixing Barium Titanate (BT) powders and PVDF-TrFE (PVDF:TrFE = 60:40 mol%) on dimethylformamide medium. This precursor solution was dried and crystallized at 100oC for 12 hours. Composites membranes were obtained by following methods: solvent casting (SC), spincoating (SP), solvent extraction by water addition (WS) and hot pressing (HP). The microstructural analysis performed by SEM showed connectivity type 3-0 and 3-1 with high homogeneity for samples of ceramic volume fraction major than 0.50. Powder agglomerates within the polymer matrix was evidenced were observed for composites with the BT volume fraction major than 40%. The composite of ceramic fraction of 0.55 presented the best values of remanent polarization (~33mC/cm2), but the flexibility of these composites with the larger ceramic fraction was significantly affected. For in vivo evaluation PVDF-TrFE/BT 90/10 membranes with 3cm larger were longitudinally implanted under tibiae of male rabbit. After 21 days the animals were sacrificed. By histological analyses were observed neo formed bone with a high mitotic activity. In the interface bone-membrane was evidenced a pronounced callus formation. These results encourage further applications of these membranes in bone-repair process.

  16. Physical Properties of the Transiting Planetary System TrES-3

    Science.gov (United States)

    Lee, Jae Woo; Youn, Jae-Hyuck; Kim, Seung-Lee; Lee, Chung-Uk; Koo, Jae-Rim

    2011-02-01

    We present four new transits of the planetary system TrES-3 observed between 2009 May and 2010 June. Among these, the third transit by itself indicates possible evidence for brightness disturbance, which might be a result of the planet blocking a cool starspot on the stellar surface. A total of 109 transit times, including our measurements, were used to determine the improved ephemeris with a transit epoch of 2454185.910944±0.000072 HJED and an orbital period of 1.30618700±0.00000015 d. We analyzed the transit light curves using the JKTEBOP code and adopting the quadratic limb-darkening law. In order to derive the physical properties of the TrES-3 system, the transit parameters were combined with the empirical relations from eclipsing binary stars and stellar evolutionary models. The stellar mass and radius obtained from a calibration using TA, logρA, and [Fe/H] are consistent with those from an isochrone analysis. We found that the exoplanet TrES-3b has a mass of 1.93±0.07MJup, a radius of 1.30±0.04RJup, a surface gravity of log gb = 3.45±0.02, a density of 0.82±0.06ρJup, and an equilibrium temperature of 1641±23K. The results are in good agreement with theoretical models for gas giant planets.

  17. As três categorias peircianas e os três registros lacanianos The three Peirce’s categories and the three Lacan’s registers

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    Maria Lucia Santaella Braga

    1999-01-01

    Full Text Available A primeira parte deste artigo propõe uma comparação geral entre as categorias fenomonológicas universais de Peirce, primeiridade, secundidade e terceiridade, de um lado, e os três registros lacanianos, também chamados de categorias conceituais da realidade humana, as categorias do imaginário, real e simbólico, de outro lado. A segunda parte avança alguns passos na análise comparativa entre Peirce e Lacan, ao discutir que a lógica da terceira categoria, que é a lógica do signo, pode funcionar como um mapa de orientação para o entendimento das interações complexas que os três registros, imaginário, real e simbólico, mantêm entre si.The first part of this paper proposes a general comparison between Peirce’s three universal phenomenological categories of firstness, secondness, and thirdness on one hand and Lacan’s three registers, also called the conceptual categories of human reality, the categories of the imaginary, the real, and the symbolic on the other hand. The second part develops the comparative analysis between Peirce and Lacan even further, showing that the logic of the third category, which is the logic of the sign, can function as a guiding map towards the understanding of the complex interactions that the three registers, the imaginary, the real, and the simbolic, have with each other.

  18. Peter Szondi e as visões do trágico na modernidade

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    Markus Lasch

    2014-09-01

    Full Text Available Partindo de uma das perguntas centrais do Ensaio sobre o trágico, de Peter Szondi, — se as filosofias do trágico, que perpassam o pensamento alemão de 1795 a 1915, de Schelling a Scheler, teriam tomado o lugar da própria tragédia — e articulando essa ideia com outros textos sobre tragédia e trágico, como os de George Steiner, Raymond Williams e Christoph Menke, o artigo discute a pertinência da manutenção do conceito do trágico para se pensar os tempos moderno e contemporâneo. Abstract: Starting from one of the main points of Peter Szondi’s An Essay on the Tragic — whether the philosophies of the tragic, which are proper to nineteenth-century German philosophy, from Schelling to Scheler, take the place of tragic poetry — and articulating this idea with other texts about tragedy and the tragic, such as the essays of George Steiner, Raymond Williams and Christoph Menke, this article discusses the pertinence of maintaining the concept of the tragic to reflect on modern and contemporary times.Keywords: tragedy; the tragic; Peter Szondi; modernity Markus Lasch é Professor Adjunto da Universidade Federal de São Paulo. Graduou-se em Letras (1994 e Linguística (1993 pela Universidade Estadual de Campinas e obteve mestrado em Teoria e História Literária pela mesma intstituição (1997. É doutor em Literatura Geral e Comparada pela Universidade Livre de Berlim (2005 e fez estágio de pós-doutorado novamente pela Universidade Estadual de Campinas. Atua predominantemente nos campos de Teoria Literária e Literatura Comparada, notadamente das literaturas em língua alemã e portuguesa. Seus enfoques de pesquisa envolvem a relação entre literatura e psicanálise, questões de sujeito e de subjetividade, tragédia e trágico, a literatura de internato, a representação da violência e do outsider na literatura, bem como a literatura e(m fontes primárias (manuscritos autógrafos e demais documentos encontráveis em arquivos

  19. CaMKIIdelta Subtypes: Localization and Function

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    Charles Burdis Burns Gray

    2014-02-01

    Full Text Available In this review we will discuss the localization and function of the known subtypes of calcium/calmodulin dependent protein kinase IIδ (CaMKIIδ and their role in cardiac physiology and pathophysiology. The CaMKII holoenzyme is comprised of multiple subunits that are encoded by four different genes called CaMKIIα, β, γ, and δ. While these four genes have a high degree of sequence homology, they are expressed in different tissues. CaMKIIα and β are expressed in neuronal tissue while γ and δ are present throughout the body, including in the heart. Both CaMKIIγ and δ are alternatively spliced in the heart to generate multiple subtypes. CaMKIIδ is the predominant cardiac isoform and is alternatively spliced in the heart to generate the CaMKIIδB subtype or the slightly less abundant δC subtype. The CaMKIIδB mRNA sequence contains a 33bp insert not present in δC that codes for an 11-amino acid nuclear localization sequence (NLS. This review will focus on the localization and function of the CaMKIIδ subtypes δB and δC and the role of these subtypes in arrhythmias, contractile dysfunction, gene transcription, and the regulation of Ca2+ handling.

  20. Ca2+ homeostasis regulates Xenopus oocyte maturation.

    Science.gov (United States)

    Sun, Lu; Hodeify, Rawad; Haun, Shirley; Charlesworth, Amanda; MacNicol, Angus M; Ponnappan, Subramaniam; Ponnappan, Usha; Prigent, Claude; Machaca, Khaled

    2008-04-01

    In contrast to the well-defined role of Ca2+ signals during mitosis, the contribution of Ca2+ signaling to meiosis progression is controversial, despite several decades of investigating the role of Ca2+ and its effectors in vertebrate oocyte maturation. We have previously shown that during Xenopus oocyte maturation, Ca2+ signals are dispensable for entry into meiosis and for germinal vesicle breakdown. However, normal Ca2+ homeostasis is essential for completion of meiosis I and extrusion of the first polar body. In this study, we test the contribution of several downstream effectors in mediating the Ca2+ effects during oocyte maturation. We show that calmodulin and calcium-calmodulin-dependent protein kinase II (CAMK2) are not critical downstream Ca2+ effectors during meiotic maturation. In contrast, accumulation of Aurora kinase A (AURKA) protein is disrupted in cells deprived of Ca2+ signals. Since AURKA is required for bipolar spindle formation, failure to accumulate AURKA may contribute to the defective spindle phenotype following Ca2+ deprivation. These findings argue that Ca2+ homeostasis is important in establishing the oocyte's competence to undergo maturation in preparation for fertilization and embryonic development.

  1. Kissankello vai kissan kello – Suomen yhdyssanojen prosodisista ominaisuuksista

    OpenAIRE

    Virkkunen, Päivi

    2015-01-01

    Tavoitteet: Tutkimuksen tavoitteena on selvittää suomen kielen yhdyssanojen tuottamista. Suomen kielen prosodiaa ja kontrastiivisen lausepainon vaikutusta puheentuottoon on tutkittu runsaasti, mutta yhdyssanojen tuottamista ei ole tutkittu tämänkaltaisilla koeasetelmilla. Tässä työssä tutkittiin, oliko puheen prosodisissa ominaisuuksissa eroja yhdyssanojen ja vertailuaineistona olleiden sanaliittojen välillä. Lisäksi tarkasteltiin lausepainon aiheuttaman prosodisen fokuksen vaikutusta yhdyssa...

  2. Aonde vai a teoria política?

    Directory of Open Access Journals (Sweden)

    Terence Ball

    2004-11-01

    Full Text Available Neste texto o autor propõe uma reavaliação da Teoria Política, a partir da constatação de sua "morte" e, ao mesmo tempo, da revalorização da disciplina a partir de meados dos anos 1970. A resposta do autor consiste em estabelecer uma distinção entre teorizações de primeira e segunda ordens, isto é, entre reflexões sobre as condições gerais de vida política em comum e o estudo dessas reflexões. Em seguida, o autor indica como os fatores que desvalorizaram a teorização normativa em décadas anteriores - em particular a Ciência Política comportamentalista - declinaram, ao mesmo tempo em que novas questões relativas à vida política em comum (nos Estados Unidos: a Guerra do Vietnã, o movimento pelos direitos civis, o movimento feminista puseram na ordem do dia a disciplina da Teoria Política. O autor encerra o texto lembrando a importância do trabalho em conjunto dos cientistas políticos "empíricos" e dos teóricos políticos "normativos", como sendo relativos a dois aspectos estreitamente relacionados de um mesmo fenômeno - como cientistas e como cidadãos.

  3. Populaarimusiikki elokuvassa : tarinankerronnan vai markkinoinnin työkalu?

    OpenAIRE

    Puurtinen, Ville-Eemeli

    2017-01-01

    Aihe tähän opinnäytetyöhön syntyi henkilökohtaisesta kiinnostuksesta elokuviin, populaarimusiikkiin sekä populaarimusiikin käyttöön elokuvien tarinankerronnassa. Tavoitteena oli tutkia ja selvittää miten populaarimusiikkia on käytetty ja käytetään nykyään elokuvien musiikkisuunnittelussa, sekä selvittää miten musiikkia lisensoidaan käytettäväksi elokuvassa. Työssä käydään läpi populaarimusiikin roolia länsimaisissa elokuvatuotannoissa, aloittaen sen historiasta ja päätyen nykypäivään. Aiheese...

  4. Vedestä vai Hengestä? : Uuras Saarnivaaran kasteteologia

    OpenAIRE

    Väisänen, Matti Kalevi

    2007-01-01

    Of water or the Spirit? Uuras Saarnivaara s theology of baptism The aim of the study was to investigate PhD and ThD Uuras Saarnivaara s views on baptism as well as their possible changes and the reasons for them. Dr Saarnivaara said himself that he searched for the truth about the relationship between baptism and faith for decades, and had faltered in his views. The method of this research is systematic analysis. A close study of the source material shows that Dr Saarnivaara s vi...

  5. KNX-tekniikka vai perinteinen sähkö

    OpenAIRE

    Reunanen, Joni

    2012-01-01

    Tässä työssä suunniteltiin kolmelle eri sähköluokitustasolle esimerkkisuunnitelma KNX-tekniikalla. Kaikkia kolmea on verrattu perinteiseen sähköasennusmenetelmään. Työstä selviävät KNX-tekniikan hyödyt ja mahdollisuudet kiinteistöautomaatiossa. Eri säh-kötasoille tehdyissä suunnitelmissa lähtökohtana on ollut hintaluokka ja tasoluokille annetut ohjeistukset. Työssä on myös esitetty esimerkkisuunnitelma KNX- tekniikalla tehdyn noin 150 m2 omakotitalon sähköistyksestä. Omakotitalo suunnitel...

  6. Heittopussi vai asiakas? : lastensuojelulaitokseen sijoitettujen nuorten kokemuksia osallisuudesta ja vaikutusmahdollisuuksista

    OpenAIRE

    Kaarto, Taru; Mäkelä, Sanna

    2009-01-01

    Tämän opinnäytetyön tarkoituksena oli selvittää lastensuojelulaitokseen sijoitettujen nuorten kokemuksia siitä, kuinka paljon he kokevat saavansa osallistua itseään koskeviin asioihin, sekä kokivatko he sen riittäväksi ja millaista vaikuttaminen ja osallistuminen oli. Lisäksi halusimme selvittää nuorten kokemuksia nuoren edun toteutumisesta. Tutkimusote oli kvalitatiivinen eli laadullinen. Tutkimusaineiston tiedonkeruumenetelmänä käytettiin puolistrukturoitua teemahaastattelua. Tutkimusa...

  7. Purgailis – putns, augs vai pārpratums?

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    Ojārs Bušs

    2012-04-01

    Full Text Available Purgailis – A BIRD, A PLANT OR A MISUNDERSTANDING?SummaryPurgailis is a quite common Latvian surname, Purgaiļi is a frequently used place-name (mostly a name of a farmstead. The names are widely associated with Latv. purvs ‘a marsh’ and gailis ‘a cock’, however, in Latvian there is no common name *purgailis. The Estonian borrowing of the Baltic origin (sookail ‘Ledum palustre’ (cf. Est. soo ‘a marsh’ and the calque sookikas ‘id.’ (cf. Est. kikas ‘a cock’ is a result of the Latvian linguistic influence (although cf. Lith. gailiai ‘Ledum palustre’, thus there is a real possibility that Latvian might have had (a quite long time ago a common name *purgaiļi ‘Ledum palustre’.

  8. Cloning goes to the movies A clonagem vai ao cinema

    Directory of Open Access Journals (Sweden)

    Craig Cormick

    2006-10-01

    Full Text Available Public attitude research conducted by Biotechnology Australia shows that one of the major sources of information on human reproductive cloning is movies. Traditionally, understanding of new and emerging technologies has come through the mass media but human cloning, being so widely addressed through the popular culture of movies, is more effectively defined by Hollywood than the news media or science media. But how well are the science and social issues of cloning portrayed in box office hits such as The Island, Multiplicity, Star Wars: Attack of the Clones and Jurassic Park? These movies have enormous reach and undoubted influence, and are therefore worth analyzing in some detail. This study looks at 33 movies made between 1971 and 2005 that address human reproductive cloning, and it categorizes the films based on their genre and potential influence. Yet rather than simply rating the quality of the science portrayed, the study compares the key messages in these movies with public attitudes towards cloning, to examine the correlations.Pesquisa de opinião pública conduzida pela Biotechnology Australia mostra que o cinema é uma das maiores fontes de informação sobre clonagem humana. Tradicionalmente, o conhecimento de tecnologias emergentes se dá através dos meios de comunicação, mas a clonagem humana, sendo tão amplamente difundida pela popular cultura cinematográfica, é definida por Hollywood de forma faz eficaz do que pelos noticiários ou publicações científicas. Mas como é que as questões sociais e científicas da clonagem são retratadas em sucessos de bilheteria como A Ilha, Multiplicidade, Guerra nas Estrelas: Ataque dos Clones e Parque dos Dinossauros? Esses filmes tiveram grande alcance e influência inquestionável e, portanto, merecem uma análise mais detalhada. O presente estudo examina 33 filmes sobre clonagem humana feitos entre 1971 e 2005 e classifica os filmes por gênero e potencial de influência. No entanto, em vez de simplesmente avaliar a qualidade da ciência retratada, o estudo compara as mensagens-chave desses filmes com a opinião pública frente a clonagem, a fim de examinar correlações.

  9. Ammattikorkeakoulut Facebookissa. Yhteisöllisyyttä vai markkinointia.

    OpenAIRE

    Lehtipää, Jenna

    2011-01-01

    Tämä opinnäytetyö on selvitys Facebookista ja sen mahdollisuuksista markkinoin-tiviestinnän välineenä. Tarkoituksena on selvittää Facebookin hyödyntämistä yh-teisöllisestä ja markkinoinnillisesta näkökulmasta ja tarjota tutkimukseni avulla havainnoimiani kehittämisehdotuksia työni toimeksiantajalle, Vaasan ammatti-korkeakoululle. Sosiaalisen median hyödyntäminen ammattikorkeakouluissa on lisääntymässä ja Vaasan ammattikorkeakoulu on liittynyt Facebookiin työni te-kemisen aikana, joten aiheeni...

  10. Ca2+ cycling in heart cells from ground squirrels: adaptive strategies for intracellular Ca2+ homeostasis.

    Directory of Open Access Journals (Sweden)

    Xiao-Chen Li

    Full Text Available Heart tissues from hibernating mammals, such as ground squirrels, are able to endure hypothermia, hypoxia and other extreme insulting factors that are fatal for human and nonhibernating mammals. This study was designed to understand adaptive mechanisms involved in intracellular Ca(2+ homeostasis in cardiomyocytes from the mammalian hibernator, ground squirrel, compared to rat. Electrophysiological and confocal imaging experiments showed that the voltage-dependence of L-type Ca(2+ current (I(Ca was shifted to higher potentials in ventricular myocytes from ground squirrels vs. rats. The elevated threshold of I(Ca did not compromise the Ca(2+-induced Ca(2+ release, because a higher depolarization rate and a longer duration of action potential compensated the voltage shift of I(Ca. Both the caffeine-sensitive and caffeine-resistant components of cytosolic Ca(2+ removal were more rapid in ground squirrels. Ca(2+ sparks in ground squirrels exhibited larger amplitude/size and much lower frequency than in rats. Due to the high I(Ca threshold, low SR Ca(2+ leak and rapid cytosolic Ca(2+ clearance, heart cells from ground squirrels exhibited better capability in maintaining intracellular Ca(2+ homeostasis than those from rats and other nonhibernating mammals. These findings not only reveal adaptive mechanisms of hibernation, but also provide novel strategies against Ca(2+ overload-related heart diseases.

  11. Ca isotope fractionation on the moon

    Science.gov (United States)

    Russell, W. A.; Papanastassiou, D. A.; Tombrello, T. A.; Epstein, S.

    1977-01-01

    Ca has been measured in a lunar soil in order to establish the presence of isotopically mass-fractionated components. Ca was extracted by a series of water leaches after the soils were 'activated' by brief exposures to fluorine gas. The O2 obtained by this fluorination is found to have delta (O-18) of +21 per mil and to be, therefore, significantly mass-fractionated. Ca obtained in the leaches was analyzed using the double-spike technique. Very small Ca isotope fractionation is found in the leaches of this soil of up to 1 per mil per mass unit difference. The small Ca effects are in marked contrast to the measured delta (O-18) for the same sample and to large effects observed in many soils for oxygen, silicon, sulfur, and potassium. The data on Ca provide stringent constraints on models which attempt to explain the isotope mass-fractionation effects in lunar soils.

  12. Cell Biology of Ca2+-Triggered Exocytosis

    OpenAIRE

    Pang, Zhiping P.; Südhof, Thomas C.

    2010-01-01

    Ca2+ triggers many forms of exocytosis in different types of eukaryotic cells, for example synaptic vesicle exocytosis in neurons, granule exocytosis in mast cells, and hormone exocytosis in endocrine cells. Work over the last two decades has shown that synaptotagmins function as the primary Ca2+-sensors for most of these forms of exocytosis, and that synaptotagmins act via Ca2+-dependent interactions with both the fusing phospholipid membranes and the membrane fusion machinery. However, some...

  13. SR-targeted CaMKII inhibition improves SR Ca2+ handling, but accelerates cardiac remodeling in mice overexpressing CaMKIIδC

    Science.gov (United States)

    Huke, Sabine; DeSantiago, Jaime; Kaetzel, Marcia A.; Mishra, Shikha; Brown, Joan H.; Dedman, John R.; Bers, Donald M.

    2010-01-01

    Cardiac myocyte overexpression of CaMKIIδC leads to cardiac hypertrophy and heart failure (HF) possibly caused by altered myocyte Ca2+ handling. A central defect might be the marked CaMKII-induced increase in diastolic sarcoplasmic reticulum (SR) Ca2+ leak which decreases SR Ca2+ load and Ca2+ transient amplitude. We hypothesized that inhibition of CaMKII near the SR membrane would decrease the leak, improve Ca2+ handling and prevent the development of contractile dysfunction and HF. To test this hypothesis we crossbred CaMKIIδC overexpressing mice (CaMK) with mice expressing the CaMKII-inhibitor AIP targeted to the SR via a modified phospholamban (PLB)-transmembrane-domain (SR-AIP). There was a selective decrease in the amount of activated CaMKII in the microsomal (SR/membrane) fraction prepared from these double-transgenic mice (CaMK/SR-AIP) mice. In ventricular cardiomyocytes from CaMK/SR-AIP mice, SR Ca2+ leak, assessed both as diastolic Ca2+ shift into SR upon tetracaine in intact myocytes or integrated Ca2+ spark release in permeabilized myocytes, was significantly reduced. The reduced leak was accompanied by enhanced SR Ca2+ load and twitch amplitude in double-transgenic mice (vs. CaMK), without changes in SERCA expression or NCX function. However, despite the improved myocyte Ca2+ handling, cardiac hypertrophy and remodeling was accelerated in CaMK/SR-AIP and cardiac function worsened. We conclude that while inhibition of SR localized CaMKII in CaMK mice improves Ca2+ handling, it does not necessarily rescue the HF phenotype. This implies that a non-SR CaMKIIδC exerts SR-independent effects that contribute to hypertrophy and HF, and this CaMKII pathway may be exacerbated by the global enhancement of Ca transients. PMID:20971119

  14. Protein Phosphatase 2A Dephosphorylates CaBP4 and Regulates CaBP4 Function

    Science.gov (United States)

    Haeseleer, Françoise; Sokal, Izabela; Gregory, Frederick D.; Lee, Amy

    2013-01-01

    Purpose. CaBP4 is a neuronal Ca2+-binding protein that is expressed in the retina and in the cochlea, and is essential for normal photoreceptor synaptic function. CaBP4 is phosphorylated by protein kinase C zeta (PKCζ) in the retina at serine 37, which affects its interaction with and modulation of voltage-gated Cav1 Ca2+ channels. In this study, we investigated the potential role and functional significance of protein phosphatase 2A (PP2A) in CaBP4 dephosphorylation. Methods. The effect of protein phosphatase inhibitors, light, and overexpression of PP2A subunits on CaBP4 dephosphorylation was measured in in vitro assays. Pull-down experiments using retinal or transfected HEK293 cell lysates were used to investigate the association between CaBP4 and PP2A subunits. Electrophysiologic recordings of cotransfected HEK293 cells were performed to analyze the effect of CaBP4 dephosphorylation in modulating Cav1.3 currents. Results. PP2A inhibitors, okadaic acid (OA), and fostriecin, but not PP1 selective inhibitors, NIPP-1, and inhibitor 2, block CaBP4 dephosphorylation in retinal lysates. Increased phosphatase activity in light-dependent conditions reverses phosphorylation of CaBP4 by PKCζ. In HEK293 cells, overexpression of PP2A enhances the rate of dephosphorylation of CaBP4. In addition, inhibition of protein phosphatase activity by OA increases CaBP4 phosphorylation and potentiates the modulatory effect of CaBP4 on Cav1.3 Ca2+ channels in HEK293T cells. Conclusions. This study provides evidence that CaBP4 is dephosphorylated by PP2A in the retina. Our findings reveal a novel role for protein phosphatases in regulating CaBP4 function in the retina, which may fine tune presynaptic Ca2+ signals at the photoreceptor synapse. PMID:23341017

  15. Supralinear dendritic Ca2+ signalling in young developing CA1 pyramidal cells

    Science.gov (United States)

    Pohle, Jörg; Bischofberger, Josef

    2014-01-01

    Although Ca2+ is critically important in activity-dependent neuronal development, not much is known about the regulation of dendritic Ca2+ signals in developing neurons. Here, we used ratiometric Ca2+ imaging to investigate dendritic Ca2+ signalling in rat hippocampal pyramidal cells during the first 1–4 weeks of postnatal development. We show that active dendritic backpropagation of Nav channel-dependent action potentials (APs) evoked already large dendritic Ca2+ transients in animals aged 1 week with amplitudes of ∼150 nm, similar to the amplitudes of ∼160 nM seen in animals aged 4 weeks. Although the AP-evoked dendritic Ca2+ load increased about four times during the first 4 weeks, the peak amplitude of free Ca2+ concentration was balanced by a four-fold increase in Ca2+ buffer capacity κs (∼70 vs. ∼280). Furthermore, Ca2+ extrusion rates increased with postnatal development, leading to a slower decay time course (∼0.2 s vs. ∼0.1 s) and more effective temporal summation of Ca2+ signals in young cells. Most importantly, during prolonged theta-burst stimulation dendritic Ca2+ signals were up to three times larger in cells at 1 week than at 4 weeks of age and much larger than predicted by linear summation, which is attributable to an activity-dependent slow-down of Ca2+ extrusion. As Ca2+ influx is four-fold smaller in young cells, the larger Ca2+ signals are generated using four times less ATP consumption. Taken together, the data suggest that active backpropagations regulate dendritic Ca2+ signals during early postnatal development. Remarkably, during prolonged AP firing, Ca2+ signals are several times larger in young than in mature cells as a result of activity-dependent regulation of Ca2+ extrusion rates. PMID:25239458

  16. Electrochemical performance in the hydrogen evolution reaction of Ni-TR (TR= La, Ce) materials synthesized using the solid state reaction method; Desempeno electroquimico en la reaccion de evolucion de hidrogeno de materiales de electrodo Ni-TR (TR = La, Ce) sintetizados por el metodo de reaccion de estado solido

    Energy Technology Data Exchange (ETDEWEB)

    Torres-Huerta, A. M.; Dominguez-Crespo, M. A.; Ramirez-Meneses, E.; Yanez-Zamora, C. [CICATA, IPN, Altamira, Tamaulipas (Mexico); Avila-Garcia, I. [IPN, ESIQIE, UPALM, Mexico, D.F. (Mexico)]. E-mail: mdominguezc@ipn.mx; adcrespo2000@yahoo.com.mx

    2009-09-15

    At the industrial level, the use of fuel cell technology is still limited because of the high costs of its parts and costs related to its operations. Although the electrode material with greater electroactivity is Pt, because of its high cost, alternative electrocatalysts have been sought that balance cost and activity. One of the materials that have been most widely used is nickel, along with some of its alloys. This material has shown good performance using low overpotentials in traditional reactions such as hydrogen (HER) and oxygen (OER) evolution, as well as high resistance to corrosion and low costs. In particular, binary and ternary alloys have shown significant increases in HER activity when compared to materials in the pure or massive state. Therefore, in the search for new alternatives with acceptable efficiency and low-cost, this work obtained Ni-TR (TR = La, Ce) using solid-state reaction with metallic acetylacetonates and metallic powder. These materials were synthesized for 3 h at different temperatures (795 or 920, 1000 and 1200 degrees Celsius) in order to evaluate the effect on the electrochemical performance of the electrocatalysts. The structural and morphological characterization of materials was performed with XRD and SEM techniques, respectively. In addition, the electrochemical performance of electrode materials was evaluated with HER using cyclic voltametry (CV) and potentiodynamic curves. The results obtained show that a combination of oxides was obtained (NiO, CeO{sub 2} and LaNiO{sub 3}) at low temperatures; nonetheless, as the synthesis temperatures increase, NiO-CeO{sub 2} and NiO-LaNiO{sub 3} alloys are formed, respectively. A clear dependence was also observed between electrocatalytic activity and the source for obtaining these materials(Ni-TR). [Spanish] A nivel industrial, el uso de la tecnologia de celdas de combustible esta todavia limitada debido sobre todo a los altos costos de las partes que la constituyen y los costos

  17. Gümnaasiumi trükkal ja keskvõimu korralduste publitseerimine 17. sajandi lõpu Tallinnas / Kaarel Vanamölder

    Index Scriptorium Estoniae

    Vanamölder, Kaarel, 1981-

    2011-01-01

    Trükkal Christoph Brendekenist. Trükkali sissetulekutest ja töö est küsitavast tasust. Plakatite tiraažist. Kindralkuberneri kantselei publitseerimisrutiinist ning korralduste edastamise kiirusest

  18. GPS data til undersøgelse af trængsel

    DEFF Research Database (Denmark)

    Andersen, Ove; Krogh, Benjamin Bjerre; Torp, Kristian

    GPS data fra køretøjer er i en årrække blevet brugt som grundlag for beregning af køretider [1] [2] og estimering af trængsel [3] . Fælles for de tidligere tiltag er, at der typisk er anvendt både kommercielt software og et kommercielt digitalt kort. Sådanne kommercielle løsninger kræver, at brug......GPS data fra køretøjer er i en årrække blevet brugt som grundlag for beregning af køretider [1] [2] og estimering af trængsel [3] . Fælles for de tidligere tiltag er, at der typisk er anvendt både kommercielt software og et kommercielt digitalt kort. Sådanne kommercielle løsninger kræver....... Open-source og creative-commons licenserne gør, at information frit kan distribueres. Som et eksempel anvendes køretidskortet til at estimere køretider for flex-transport for store dele af Danmark. Artiklen er struktureret som følgende. Først præsenteres, hvorledes GPS data og OSM kortet er anvendt til...... at skabe et hastigheds- og trængselskort for hele Danmark. Herefter vises det, hvorledes GPS data inddelt i ture kan anvendes til at analysere kortere strækninger (nogle kilometer) f.eks. hvor der skal være grønne bølger. Slutteligt vises, hvordan GPS data kan anvendes til at nærstudere et enkelt kryds...

  19. A Spitzer five-band analysis of the Jupiter-sized planet TrES-1

    Energy Technology Data Exchange (ETDEWEB)

    Cubillos, Patricio; Harrington, Joseph; Foster, Andrew S. D.; Lust, Nate B.; Hardy, Ryan A.; Bowman, M. Oliver [Planetary Sciences Group, Department of Physics, University of Central Florida, Orlando, FL 32816-2385 (United States); Madhusudhan, Nikku, E-mail: pcubillos@fulbrightmail.org [Department of Physics and Department of Astronomy, Yale University, New Haven, CT 06511 (United States)

    2014-12-10

    With an equilibrium temperature of 1200 K, TrES-1 is one of the coolest hot Jupiters observed by Spitzer. It was also the first planet discovered by any transit survey and one of the first exoplanets from which thermal emission was directly observed. We analyzed all Spitzer eclipse and transit data for TrES-1 and obtained its eclipse depths and brightness temperatures in the 3.6 μm (0.083% ± 0.024%, 1270 ± 110 K), 4.5 μm (0.094% ± 0.024%, 1126 ± 90 K), 5.8 μm (0.162% ± 0.042%, 1205 ± 130 K), 8.0 μm (0.213% ± 0.042%, 1190 ± 130 K), and 16 μm (0.33% ± 0.12%, 1270 ± 310 K) bands. The eclipse depths can be explained, within 1σ errors, by a standard atmospheric model with solar abundance composition in chemical equilibrium, with or without a thermal inversion. The combined analysis of the transit, eclipse, and radial-velocity ephemerides gives an eccentricity of e=0.033{sub −0.031}{sup +0.015}, consistent with a circular orbit. Since TrES-1's eclipses have low signal-to-noise ratios, we implemented optimal photometry and differential-evolution Markov Chain Monte Carlo (MCMC) algorithms in our Photometry for Orbits, Eclipses, and Transits pipeline. Benefits include higher photometric precision and ∼10 times faster MCMC convergence, with better exploration of the phase space and no manual parameter tuning.

  20. El espectro de KX TrA entre 1990 y 1996

    Science.gov (United States)

    Brandi, E.; García, L.; Ferrer, O.; Barbá, R.

    La estrella simbiótica KX TrA = He2-177 fue observada espectroscópicamente con el telescopio de 2.15 m del CASLEO entre los años 1990 y 1996, utilizándose resoluciones intermedia y alta. El rango espectral estudiado se extiende desde 4400 Åa 7200 Å. La historia fotométrica de KX TrA presenta explosiones del mismo tipo que las mostradas por la nova lenta RR Tel y su espectro de alta excitación, muy rico en líneas de emisión, es también similar al de RR Tel. Por lo tanto, es importante analizar la evolución espectral de KX TrA en el tiempo, prestando especial atención a los posibles cambios en los niveles de excitación. Las emisiones presentes corresponden a transiciones permitidas y prohibidas con un amplio rango de ionización, incluyéndose las anchas e intensas líneas originadas por scattering Raman de O VI en λλ 6825 y 7082 Å. En el período cubierto por nuestras observaciones se estudia la variación de la intensidad relativa de las emisiones, el comportamiento de las velocidades radiales y los cambios de perfiles de las líneas, especialmente en Hα y Hβ. El contínuo de la región roja observada indica un tipo espectral no más tardío que M3 para la componente gigante fría del sistema.

  1. Preferred deposition of phospholipids onto ferroelectric P(VDF-TrFE) films via polarization patterning

    Energy Technology Data Exchange (ETDEWEB)

    Heredia, A; Machado, M; Kholkin, A L [Department of Ceramics and Glass Engineering and CICECO, University of Aveiro, 3810-193Aveiro (Portugal); Bdikin, I K; Gracio, J [Nanotechnology Research Division, Centre for Mechanical Technology and Automation, University of Aveiro, 3810-193 Aveiro (Portugal); Yudin, S; Fridkin, V M [Institute of Crystallography, RAS, 933333 Moscow (Russian Federation); Delgadillo, I, E-mail: alejandro.heredia@ua.p [Department of Chemistry and QOPNA, University of Aveiro, 3810-193 Aveiro (Portugal)

    2010-08-25

    Ferroelectric polarization can be used to assemble various organic and inorganic species and to create nanostructures with controlled properties. In this work, we used poly(vinylidene fluoride-co-trifluoroethylene) P(VDF-TrFE), ultrathin films deposited by the Langmuir-Blodgett technique as templates for the assembly of various phospholipids, which are the essential components of cell membranes. It was observed that 1,2-di-O-hexadecyl-sn-glycero-3-phosphocholine phospholipids (DHPC) form self-assembled structures (molecular domains) on bare P(VDF-TrFE) surfaces. These were revealed by the formation of homogeneous and stable rounded blobs with diameters in the range 0.5-3 {mu}m. Further, ferroelectric polymer films were polarized by the application of various voltages via a conducting tip using a piezoresponse force microscopy (PFM) setup and PFM images were obtained showing controlled polarization distribution. After this, the phospholipid molecules were deposited from the solution. Conventional atomic force microscopy experiments were then performed to assess the selectivity of the deposition process. It was observed that the deposition process is very sensitive to the concentration of the solution. The selective deposition was observed mainly at the polarization boundaries where the selectivity reached a maximum value of about 20-40%. In this way, the controlled assembly of organic molecules on the polymer surfaces could be achieved. In addition, the PFM tips could be functionalized by the phospholipids and switchable lines of the DHPC molecules on the P(VDF-TrFE) surface were then visualized by PFM.

  2. Preferred deposition of phospholipids onto ferroelectric P(VDF-TrFE) films via polarization patterning

    Science.gov (United States)

    Heredia, A.; Machado, M.; Bdikin, I. K.; Gracio, J.; Yudin, S.; Fridkin, V. M.; Delgadillo, I.; Kholkin, A. L.

    2010-08-01

    Ferroelectric polarization can be used to assemble various organic and inorganic species and to create nanostructures with controlled properties. In this work, we used poly(vinylidene fluoride-co-trifluoroethylene) P(VDF-TrFE), ultrathin films deposited by the Langmuir-Blodgett technique as templates for the assembly of various phospholipids, which are the essential components of cell membranes. It was observed that 1,2-di-O-hexadecyl-sn-glycero-3-phosphocholine phospholipids (DHPC) form self-assembled structures (molecular domains) on bare P(VDF-TrFE) surfaces. These were revealed by the formation of homogeneous and stable rounded blobs with diameters in the range 0.5-3 µm. Further, ferroelectric polymer films were polarized by the application of various voltages via a conducting tip using a piezoresponse force microscopy (PFM) setup and PFM images were obtained showing controlled polarization distribution. After this, the phospholipid molecules were deposited from the solution. Conventional atomic force microscopy experiments were then performed to assess the selectivity of the deposition process. It was observed that the deposition process is very sensitive to the concentration of the solution. The selective deposition was observed mainly at the polarization boundaries where the selectivity reached a maximum value of about 20-40%. In this way, the controlled assembly of organic molecules on the polymer surfaces could be achieved. In addition, the PFM tips could be functionalized by the phospholipids and switchable lines of the DHPC molecules on the P(VDF-TrFE) surface were then visualized by PFM.

  3. Nonvolatile transtance change random access memory based on magnetoelectric P(VDF-TrFE)/Metglas heterostructures

    Science.gov (United States)

    Lu, Peipei; Shang, Dashan; Shen, Jianxin; Chai, Yisheng; Yang, Chuansen; Zhai, Kun; Cong, Junzhuang; Shen, Shipeng; Sun, Young

    2016-12-01

    Transtance change random access memory (TCRAM) is a type of nonvolatile memory based on the nonlinear magnetoelectric coupling effects of multiferroics. In this work, ferroelectric P(VDF-TrFE) thin films were prepared on Metglas foil substrates by the sol-gel technique to form multiferroic heterostructures. The magnetoelectric voltage coefficient of the heterostructure can be switched reproducibly to different levels between positive and negative values by applying selective electric-field pulses. Compared with bulk multiferroic heterostructures, the polarization switching voltage was reduced to 7 V. Our facile technological approach enables this organic magnetoelectric heterostructure as a promising candidate for the applications in multilevel TCRAM devices.

  4. Sexo, gênero e comportamento no trânsito

    OpenAIRE

    Carvalho, Marcos Roberto Alves de

    2015-01-01

    Orientadora: Profª Drª Alessandra Sant'Anna Bianchi Dissertação (mestrado) - Universidade Federal do Paraná, Setor de Ciências Humanas, Programa de Pós-Graduação em Psicologia. Defesa: Curitiba, 25/09/2015 Inclui referências Resumo: Os dados de acidentalidade e mortalidade no contexto do trânsito demonstram a relevância deste campo de estudo e sua configuração como um problema de saúde pública, principalmente nos países ditos em desenvolvimento. Os números falam por si só, no Brasil,...

  5. Industriellt träbyggande förenar ekonomi och byggbarhet

    OpenAIRE

    Erikshammar, Jarkko; Meiling, John; Jansson, Gustav; Levander, Erika

    2010-01-01

    Industriellt träbyggande baseras på en återupprepning av givna byggprocesser jämfört med traditionellt byggande där affären och kunden ordnas in i unika projekt. Eftersom byggprocessen består av återkommande aktiviteter kan de industriella metoderna förbättra inte bara projektering och produktion, utan de positiva effekterna stäcker sig ända in i förvaltningsfasen. Marknaden kan redan idag erbjudas kostnadseffektivare bostäder, med högre flexibilitet och bättre kvalitet genom att beställaren,...

  6. Effekt af postoperativ træning af patienter med lumbal diskusprotese

    OpenAIRE

    Svendsen, Marijke de Koning

    2010-01-01

    Design: Et randomiseret kontrolleret forsøg. Formål: At undersøge om der er forskel mellem effekt af postoperativ vejledning og effekt af postoperativ vejledning + fysioterapeut-ledet træning af patienter med 1-2 lumbale diskusproteser, målt på smerte og funktion, 3 måneder postoperativt i forhold til præoperativt. Baggrund: Operation med indsættelse af diskusprotese til patienter med non-specifikke kroniske lænderygsmerter har været en anvendt behandlingsmodalitet i Europa s...

  7. Medida de la congestión del tráfico en ciudades

    OpenAIRE

    Campos Cacheda, Jose Magin; Robusté Antón, Francesc

    2011-01-01

    La congestión del tráfico en entornos urbanos y metropolitanos perturba la eficiencia de la logística de la ciudad contemplada como “fábrica de movilidad”, afectando a todos los colectivos sociales de forma directa e indirecta, empeorando la calidad de vida y penalizando las actividades comerciales y el acceso y entrega de productos en “la última milla”. Esta investigación define indicadores objetivos simples y sus homólogos subjetivos (a partir de la percepción de los usuarios) d...

  8. Ultra-širokopojasni sustav s ortogonalno kodiranim diferencijalnim TR prijemnikom

    OpenAIRE

    Milanović, Josip; Rimac-Drlje, Snježana; Rupčić, Slavko

    2015-01-01

    U ovom je radu predstavljen kodirani diferencijalni TR (cDTR) prijemnik koji mapiranjem informacije u K različitih ortogonalnih kodova omogućuje prijenos dodatnog bita. Svojstva kodiranog DTR-a su analizirana i uspoređena s konvencionalnim DTR-om. Kroz analizu je dana ocjena svojstva prijemnika u smislu vjerojatnosti pogreške bita (BEP), brzine prijenosa i složenosti prijemnika. Rezultati su pokazali da kodirani DTR uz isti BEP omogućuje veću brzinu prijenosa od konvencionalnog DTR-a. Osim to...

  9. Gåsetrekket i Vesterålen og Nord-Trøndelag 2004

    DEFF Research Database (Denmark)

    Tombre, I.; Madsen, J.; Bakken, J.

    After several years of conflicts between spring staging geese and agricultural interests in Norway, 1 million NOK was provided by the authorities in order to carry out various alleviating initiatives in 2004. Hence, the Governors of Nord-Trøndelag and Nordland initiated a pilot project for the go...... neighbouring properties practice the same kind of action (scaring or free-areas). This will allow a more efficient scaring regime at the same time as the geese are able to fulfil their energetic needs on larger coherent and undisturbed areas...

  10. Reposição de nutrientes durante três cultivos de alface em hidroponia

    Directory of Open Access Journals (Sweden)

    Backes Fernanda Alice A.L.

    2003-01-01

    Full Text Available O experimento foi realizado em estufa plástica na UFSM, de agosto a novembro/99. Avaliou-se cinco formas de reposição de nutrientes na solução nutritiva com base na condutividade elétrica (CE, no sistema 'NFT' (Nutrient Film Technique e duas cultivares de alface: Regina e Deisy, cultivadas em bancadas de sustentação formadas por telhas de fibro-cimento revestidas com tinta betuminosa Neutrolâ. Foram dispostas 14 plantas por canal para cada repetição e uma cultivar em cada três canais, totalizando 84 plantas por bancada de produção. Comparou-se a eficiência de métodos de reposição de nutrientes na produção de alface, assim como a utilização da mesma solução, com reposição de nutrientes, durante três cultivos consecutivos. O delineamento experimental foi de blocos casualizados, com três repetições, em esquema fatorial 5x2. Os resultados demonstraram que o desempenho das cultivares avaliadas não foi influenciado pelos métodos de reposição de nutrientes. A ausência de reposição de nutrientes na solução nutritiva, durante um cultivo, permitiu maior produtividade da cultura, não se recomendando a reposição quando o objetivo for renovar a solução ao final do cultivo. As formas de reposição de nutrientes na solução nutritiva, durante o primeiro e segundo cultivos, não alteraram a produtividade da cultura em relação à renovação completa da solução nutritiva ao final do cultivo hidropônico. Para a reutilização de solução nutritiva, recomenda-se a reposição de nutrientes sempre que a CE diminuir 50% da inicial, possibilitando a produção por pelo menos três cultivos.

  11. Hemorragia subendocárdica en fallecidos debido a accidentes de tránsito

    OpenAIRE

    Daisy Virgilí Jiménez; Yadira Leal Rivero; Daisy Ferrer Marrero

    1998-01-01

    En una revisión de 13 autopsias médico legales realizadas en el Instituto de Medicina Legal en casos de fallecidos por causas violentas, específicamente vinculados con hechos de tránsito, se encontró que 5 presentaban zonas de hemorragia subendocárdica a nivel de las paredes septal y libre del ventrículo izquierdo del corazón. El resultado de estos estudios es compatible con la hipótesis de la existencia de lesiones cardíacas secundarias a enfermedades no cardíacas, particularmente mediadas p...

  12. Resultados del tratamiento a pacientes con tríada terrible del codo

    OpenAIRE

    Héctor Gabriel Díaz Carrillo; William Álvarez Consuegra; Michel Quevedo Pérez

    2015-01-01

    La luxación del codo, asociada a la fractura del proceso coronoideo y a la fractura de la cabeza radial, es denominada “tríada terrible del codo”, debido a la complejidad al abordar el tratamiento correcto y lo desalentador de los resultados que se obtienen con el mismo. Se realizó un estudio prospectivo, donde se recogen los resultados postoperatorios, obtenidos en cuatro pacientes con inestabilidad traumática aguda del codo, tratados entre enero de 2012 y mayo de 2013, que acudieron inicial...

  13. Chef, Opérations de trésorerie | CRDI - Centre de recherches pour ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    Le chef des opérations de trésorerie veille à ce que l'ensemble des rapports internes et externes (états financiers, plaques de société d'État, déclaration de renseignements à titre d'organisme de bienfaisance) soient préparés de manière précise et rapide. Le chef est chargé de la gestion des liquidités pour le siège social ...

  14. Trânsito religioso e reinvenções femininas do sagrado na modernidade

    Directory of Open Access Journals (Sweden)

    Sandra Duarte de Souza

    2009-05-01

    Full Text Available O trânsito religioso é indicador de que os “modelos oficiais e religião” têm sido substituídos, ou coexistem com  oferenciais religiosos cambiantes, respondendo à demanda dos sujeitos de fé. Os anseios de significação imediata dos sujeitos de fé encontram na “vagabundagem religiosa” sua atitude mais coerente. A vagabundagem semântica dos crentes infiéis é o elemento dinamizador do campo religioso moderno. No presente texto nos propomos a discutir o trânsito religioso de mulheres, suas motivações e as implicações dessa “andança religiosa” para a concepção que estas têm a respeito do sagrado. Para tanto nos utilizamos e dados de nossa pesquisa de campo realizada para a Universidade Católica de Goiás no ano 2000 em Alto Paraíso – GO, bem como de dados obtidos em pesquisa que realizamos para a Universidade Metodista de São Paulo na cidade de São Bernardo do Campo – SP em 2003, como parte do projeto intitulado: “Religiosidade caleidoscópica – nomadismo espiritual: implicações de gênero”. Os resultados da pesquisa indicaram um trânsito maior das mulheres por força das representações de gênero.Palavras-chave: Trânsito religioso; Secularização; Gênero.ABSTRACTReligious transit indicates that ‘official models of religion’ have been replaced, or coexist with mutable religious references, answering the believers’ demand. Their desire for immediate signification finds their most coherent attitude in ‘religious nomadism’. Unfaithful believers’ semantic nomadism is the dynamic element of modern religion. This text discusses women’s religious transit, their motivations and the implications of that ‘religious walk’ to their conception of the sacred. The date used are from a field research for the Universidade Católica de Goiás, developed in Alto Paraíso - State of Goiás, in 2000, and from a research for the Universidade Metodista de São Paulo, carried out in São Bernardo do

  15. Trådbindere til forankring af skalmure og hule mure

    DEFF Research Database (Denmark)

    Høffding Knutsson, H.

    Anvisningen er et hjælpemiddel for projekterende, udførende og kontrollerende parter ved dimensionering, montering og vurdering af trådbindere. Den indeholder bæreevnediagrammer for de gængse størrelser bindere i tinbronze og rustfri stål, både bæreevnen af retlinede bindere, men også den...... reducerede bæreevne af krumme og bøjede bindere og ved bindere i fabriksfremstillede bagvægge af beton eller letbeton....

  16. Selección trófica en Anemonia sulcata

    OpenAIRE

    Hernández Marrero, Yurena Arántzazu

    2012-01-01

    [ES] Anemonia sulcata no muestra una selección trófica en función del color del alimento, aunque sí establece una tendencia clara a alimentarse sobre restos de crustáceos frente al pescado. Además, se observó que cuanto mayor es la talla del individuo, menor tiempo invierte en desplazarse hasta el alimento. La velocidad de desplazamiento se incrementa en relación directa al tiempo transcurrido en ayunas [EN] Anemonia sulcata shows no trophic selection based on the color of the food items, ...

  17. Alergeni u mesnim proizvodima s hrvatskog tržišta

    OpenAIRE

    Poljanec, Ivna; Vahčić, Nada; Krešić, Greta; Kolarić Kravar, Sanja; Kudumija, Nina; Pleadin, Jelka

    2017-01-01

    Cilj ovog rada je utvrditi prisutnost sljedećih alergena: glutena, soje, mlijeka i gorušice u različitim vrstama mesnih proizvoda te istražiti sukladnost sa oznakama na proizvodu odnosno udovoljavanje zahtjevima zakonodavstva s obzirom na utvrđene količine. Ispitivanje je provedeno na 51 uzorku mesnih proizvoda proizvedenih od strane različitih mesnih industrija i zastupljenih na hrvatskom tržištu. Količina alergena (mg/kg) određivana je primjenom validiranih ELISA (enzyme-linked immunosorben...

  18. Prevención de accidentes de tráfico

    OpenAIRE

    Ocampo Suárez-Valdés, Gonzalo

    2010-01-01

    Uno de los objetivos prioritarios en los países industrializados consiste en lograr para los ciudadanos el mayor grado de bienestar posible. La prevención de accidentes de tráfico constituye una tarea importante dado su enorme impacto en nuestra sociedad actual, pues son conocidas las altas cifras de mortalidad y discapacidad derivadas de este tipo de accidentes en España. A pesar del alto índice de siniestros, en nuestro país se ha conseguido en los últimos años una disminución de un 31% de ...

  19. Prevención de los accidentes de tráfico infantiles

    OpenAIRE

    Rivera Menor, Estrella

    2003-01-01

    En España, aproximadamente el 3% de los muertos en accidente de tráfico es un niño de hasta 14 años. Sin embargo, con independencia de la importancia cuantitativa, es evidente la importancia cualitativa y sentimental que la pérdida de una vida que apenas comienza, o su sufrimiento, tiene sobre una sociedad que, de forma natural, acepta y asume como una de sus obligaciones, con entronque en sus valores primarios y por tanto más fuertemente arraigados, el salvaguardar y proteger a s...

  20. Triptycene induced enhancement of membrane gas selectivity for microporous Tröger's base polymers.

    Science.gov (United States)

    Carta, Mariolino; Croad, Matthew; Malpass-Evans, Richard; Jansen, Johannes C; Bernardo, Paola; Clarizia, Gabriele; Friess, Karel; Lanč, Marek; McKeown, Neil B

    2014-06-04

    A highly gas permeable polymer with exceptional size selectivity is prepared by fusing triptycene units together via a poly-merization reaction involving Tröger's base formation. The extreme rigidity of this polymer of intrinsic microporosity (PIM-Trip-TB) facilitates gas permeability data that lie well above the benchmark 2008 Robeson upper bounds for the important O2 /N2 and H2 /N2 gas pairs. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  1. Forme très amyotrophique et invalidante d'une sclérodermie ...

    African Journals Online (AJOL)

    Forme très amyotrophique et invalidante d'une sclérodermie masculine. Olfa Berriche, Zohra Elati. Abstract. La sclérodermie systémique (SS) est une maladie rare qui se caractérise par des anomalies de la microcirculation et des lésions desclérose cutanée et/ou viscérale. Les lésions de sclérose cutanée peuvent être ...

  2. Desempenho de vacas Charolês e Nelore desterneiradas aos três ou sete meses

    Directory of Open Access Journals (Sweden)

    Restle João

    2001-01-01

    Full Text Available Foi avaliado o desempenho de vacas Charolês (C e Nelore (N, agrupadas em três classes de idade, jovens (3 e 4 anos, adultas (5 a 7 anos e velhas (8 ou mais anos, desmamadas aos três (precoce ou sete meses no outono (tradicional. O peso no outono das vacas desterneiradas aos três meses (T3 foi 45 kg superior ao das vacas com remoção do bezerro aos sete meses (T7. O estado corporal aos sete meses também foi melhor nas vacas do T3 (3,3 contra 2,1 pontos. Vacas do T3 apresentaram maior ganho de peso do parto ao final do período reprodutivo e apresentaram maiores porcentagem de cio (81 contra 51% e prenhez (67,2 contra 37,3% e menor intervalo do parto ao primeiro cio pós-parto (102 contra 114 dias que vacas do T7. Vacas adultas apresentaram melhor estado corporal aos sete meses e tiveram melhor desempenho reprodutivo do que vacas velhas e jovens. A diferença na porcentagem de prenhez entre o T3 e T7 foi mais evidente nas vacas jovens (42,11 contra 12,5% e velhas (51,72 contra 35,71% que nas adultas (62,50 contra 53,33%. Vacas C foram mais pesadas que as N, ao parto, aos três e sete meses pós-parto e apresentaram melhor estado corporal aos sete meses. O efeito do desmame precoce no desempenho reprodutivo foi mais evidente nas vacas C. A porcentagem de fêmeas prenhes nas C foi de 80,60% para o T3 e 41,90% para o T7, já nas N as porcentagens foram de 45,50 e 30,00%, respectivamente, para o T3 e T7. Nas vacas C, a produção de leite e a amamentação apresentaram efeito inibidor, sobre a reprodução, mais marcante que nas vacas N.

  3. High Efficiency, Digitally Calibrated TR Modules Enabling Lightweight SweepSAR Architectures for DESDynI-Class Radar Instruments Project

    Data.gov (United States)

    National Aeronautics and Space Administration — Develop and demonstrate a next-generation digitally calibrated, highly scalable, L-band Transmit/Receive (TR) module to enable a precision beamforming SweepSAR...

  4. Behov for træning af dokumentation i sygepleje giver synergi mellem grunduddannelser i et University College

    DEFF Research Database (Denmark)

    Kollerup, Mette Geil; Nielsen, Tina

    2014-01-01

    Sygeplejerskeuddannelsen og Datamatikeruddannelsen på University College Nordjylland har samarbejdet om udvikling af en elektronisk patientjournal, som sygeplejestuderende kan anvende til at træne elektronisk dokumentation af sygeplejen i færdighedslaboratoriet. Baggrunden for projektet er...

  5. A Comparison of DSM-IV-TR and DSM-5 Diagnostic Classifications in the Clinical Diagnosis of Autistic Spectrum Disorder.

    Science.gov (United States)

    Yaylaci, Ferhat; Miral, Suha

    2017-01-01

    Aim of this study was to compare children diagnosed with Pervasive Developmental Disorder (PDD) according to DSM-IV-TR and DSM-5 diagnostic systems. One hundred fifty children aged between 3 and 15 years diagnosed with PDD by DSM-IV-TR were included. PDD symptoms were reviewed through psychiatric assessment based on DSM-IV-TR and DSM-5 criteria. Clinical severity was determined using Childhood Autism Rating Scale (CARS) and Autism Behavior Checklist (ABC). A statistically significant decrease (19.3 %) was detected in the diagnostic ratio with DSM-5. Age and symptom severity differed significantly between those who were and were not diagnosed with PDD using DSM-5. B4 criteria in DSM-5 was most common criterion. Results indicate that individuals diagnosed with PDD by DSM-IV-TR criteria may not be diagnosed using DSM-5 criteria.

  6. sA Comparison of DSM-IV-TR and DSM-5 Diagnostic Classifications in the Clinical Diagnosis of Autistic Spectrum Disorder

    Science.gov (United States)

    Yaylaci, Ferhat; Miral, Suha

    2017-01-01

    Aim of this study was to compare children diagnosed with Pervasive Developmental Disorder (PDD) according to DSM-IV-TR and DSM-5 diagnostic systems. One hundred fifty children aged between 3 and 15 years diagnosed with PDD by DSM-IV-TR were included. PDD symptoms were reviewed through psychiatric assessment based on DSM-IV-TR and DSM-5 criteria.…

  7. Ca(2+) signalling in the Golgi apparatus.

    Science.gov (United States)

    Pizzo, Paola; Lissandron, Valentina; Capitanio, Paola; Pozzan, Tullio

    2011-08-01

    The Golgi apparatus plays a central role in lipid and protein post-translational modification and sorting. Morphologically the organelle is heterogeneous and it is possible to distinguish stacks of flat cysternae (cis- and medial Golgi), tubular-reticular networks and vesicles (trans-Golgi). These morphological differences parallel a distinct functionality with a selective distribution and complementary roles of the enzymes found in the different compartments. The Golgi apparatus has been also shown to be involved in Ca(2+) signalling: it is indeed endowed with Ca(2+) pumps, Ca(2+) release channels and Ca(2+) binding proteins and is thought to participate in determining the spatio-temporal complexity of the Ca(2+) signal within the cell, though this role is still poorly understood. Recently, it has been demonstrated that the organelle is heterogeneous in terms of Ca(2+) handling and selective reduction of Ca(2+) concentration, both in vitro and in a genetic human disease, within one of its sub-compartment results in alterations of protein trafficking within the secretory pathway and of the entire Golgi morphology. In this paper we review the available information on the Ca(2+) toolkit within the Golgi, its heterogeneous distribution in the organelle sub-compartments and discuss the implications of these characteristics for the physiopathology of the Golgi apparatus. 2011 Elsevier Ltd. All rights reserved.

  8. Cuento: Sangre de caña

    Directory of Open Access Journals (Sweden)

    Segundo Benjamín Corredor

    1967-09-01

    Full Text Available El viento se convertía en murmullo contra las hojas de los cañaverales y contra los techos semipelados del rancho. También menguaba el calor que maduraba los plantíos de caña y que hacía más efusiva la sangre de los moradores.

  9. The delicate bistability of CaMKII.

    Science.gov (United States)

    Michalski, P J

    2013-08-06

    Calcium/calmodulin-dependent protein kinase II (CaMKII) is a synaptic, autophosphorylating kinase that is essential for learning and memory. Previous models have suggested that CaMKII functions as a bistable switch that could be the molecular correlate of long-term memory, but experiments have failed to validate these predictions. These models involved significant approximations to overcome the combinatorial complexity inherent in a multisubunit, multistate system. Here, we develop a stochastic particle-based model of CaMKII activation and dynamics that overcomes combinatorial complexity without significant approximations. We report four major findings. First, the CaMKII model system is never bistable at resting calcium concentrations, which suggests that CaMKII activity does not function as the biochemical switch underlying long-term memory. Second, the steady-state activation curves are either laserlike or steplike. Both are characterized by a well-defined threshold for activation, which suggests that thresholding is a robust feature of this system. Third, transiently activated CaMKII can maintain its activity over the time course of many experiments, and such slow deactivation may account for the few reports of bistability in the literature. And fourth, under in vivo conditions, increases in phosphatase activity can increase CaMKII activity. This is a surprising and counterintuitive effect, as dephosphorylation is generally associated with CaMKII deactivation. Copyright © 2013 Biophysical Society. Published by Elsevier Inc. All rights reserved.

  10. Kliniske retningslinjer for træning af aerob kapacitet og muskelstyrke til patienter med reumatoid artrit

    DEFF Research Database (Denmark)

    Larsen, Birgit Tine; Læssøe, Uffe; Kjær, Thomas

    -intensitets træning af patienter med reumatoid artrit. Evidensen forsikkerhed og effekt af høj-intensitetstræning af patienter med reumatoid artrit i funktionsklasse III er CENTER FOR KLINISKE RETNINGSLINJER- CLEARINGHOUSESide 2uklar. Der bør derfor tages ekstra forholdsregler i forbindelse med træningen af disse......Baggrund: Patienter med reumatoid artrit har en forhøjet risiko for udviklingen af cardio-vaskulære lidelser (CVD) ogtidlig død. Inflammation, smerter og generel træthed resulterer i nedsat muskelstyrke og lavt fysiskfunktions- og aktivitetsniveau hos mange af disse patienter, hvilket yderligere...... III, i primær eller sekundær sektoren. Intervention/er: Kortvarig land- eller vandbaseret højintensitets træning af aerob kapacitet, kortvarig eller længerevarendeland-baseret høj-intensitets træning af aerob kapacitet i kombination med muskelstyrke træning oglængerevarende land-baseret høj...

  11. Ground-state correlations in sup 40 Ca and sup 48 Ca

    Energy Technology Data Exchange (ETDEWEB)

    Takayanagi, K. (Tokyo Denki Univ., Dept. of Mathematical Sciences, Saitama (Japan)); Lipparini, E. (Dipt. di Fisica, Univ. di Trento, Povo (Italy))

    1992-01-13

    Second-order perturbation theory with a G-matrix is adopted to examine from a unified point of view the effects of two-particle-two-hole correlations on the matter and momentum distributions and the occupation numbers in {sup 40}Ca and {sup 48}Ca. Polarization effects induced by the neutron excess in {sup 48}Ca are investigated in detail. (orig.).

  12. Mechanical Strain Regulates Osteoblast Proliferation Through Ca(2+)-CaMK-CREB Signal Pathway.

    Science.gov (United States)

    Guo, Yong; Lv, Qi; Zou, Xian-Qiong; Yan, Zhi-Xiong; Yan, Yu-Xian

    2016-06-20

    Objective To investigate the effects of mechanical strain on Ca(2+)-calmodulin dependent kinase (CaMK)-cAMP response element binding protein (CREB) signal pathway and proliferation of osteoblasts.Methods Using a four-point bending device, MC3T3-E1 cells were exposed to mechanical tensile strains of 2500 µs and 5000 µs at 0.5 Hz respectively. The intracellular free Ca(2+) ([Ca(2+)]i) concentration and calmodulin activity were assayed by fluorospectrophotometry, CaMK II β, CREB, and phosphorylated (activated) CREB (p-CREB) were assessed by Western blot, and cells proliferation was assayed with MTT. Pretreatment with verapamil was carried out to block Ca(2+) channel, and inhibitor U73122 was used to inhibit phospholipase C (PLC).Results Mechanical strains of 2500 µs and 5000 µs for 1 to 10 minutes both increased [Ca(2+)]i level of the cells. The 2500 µs strain, a periodicity of 1 h/d for 3 days, activated calmodulin, elevated protein levels of CaMK II β and p-CREB, and promoted cells proliferation, which were attenuated by pretreatment of verapamil or U73122. The effects of 5000 µs strain on calmodulin, CaMK II β, p-CREB and proliferation were contrary to 2500 µs strain.Conclusion The mechanical strain regulates osteoblasts proliferation through Ca(2+)-CaMK-CREB signal pathway via Ca(2+) channel and PLC/IP3 transduction cascades.

  13. AMP-activated protein kinase-mediated feedback phosphorylation controls the Ca(2+)/calmodulin (CaM) dependence of Ca(2+)/CaM-dependent protein kinase kinase β.

    Science.gov (United States)

    Nakanishi, Akihiro; Hatano, Naoya; Fujiwara, Yuya; Bin Shari, Arian; Takabatake, Shota; Akano, Hiroki; Kanayama, Naoki; Magari, Masaki; Nozaki, Naohito; Tokumitsu, Hiroshi

    2017-10-03

    The Ca(2+)/calmodulin-dependent protein kinase kinase β(CaMKKβ)/5'AMP-activated protein kinase (AMPK) phosphorylation cascade affects various Ca(2+)-dependent metabolic pathways and cancer growth. Unlike recombinant CaMKKβ that exhibits higher basal activity (autonomous activity), activation of the CaMKKβ/AMPK signaling pathway requires increased intracellular Ca(2+) concentrations. Moreover, the Ca(2+)/CaM dependence of CaMKKβ appears to arise from multiple phosphorylation events, including autophosphorylation and activities furnished by other protein kinases. However, the effects of proximal downstream kinases on CaMKKβ activity have not yet been evaluated. Here, we demonstrate feedback phosphorylation of CaMKKβ at multiple residues by CaMKKβ-activated AMPK in addition to autophosphorylation in vitro, leading to reduced autonomous, but not Ca(2+)/CaM-activated, CaMKKβ activity. MS analysis and site-directed mutagenesis of AMPK phosphorylation sites in CaMKKβ indicated that Thr144 phosphorylation by activated AMPK converts CaMKKβ into a Ca(2+)/CaM-dependent enzyme, as shown by completely Ca(2+)/CaM-dependent CaMKK activity of a phosphomimetic Thr144Glu CaMKKβ mutant. CaMKKβ mutant analysis indicated that the C-terminal domain (residues 471-587) including the autoinhibitory region plays an important role in stabilizing an inactive conformation in a Thr144 phosphorylation-dependent manner. Furthermore, immunoblot analysis with antiphospho-Thr144 antibody revealed phosphorylation of Thr144 in CaMKKβ in transfected COS-7 cells that was further enhanced by exogenous expression of AMPKα. These results indicate that AMPK-mediated feedback phosphorylation of CaMKKβ regulates the CaMKKβ/AMPK signaling cascade and may be physiologically important for intracellular maintenance of Ca(2+)-dependent AMPK activation by CaMKKβ. Copyright © 2017, The American Society for Biochemistry and Molecular Biology.

  14. Ca-Dependent Folding of Human Calumenin

    Science.gov (United States)

    Mazzorana, Marco; Hussain, Rohanah; Sorensen, Thomas

    2016-01-01

    Human calumenin (hCALU) is a six EF-hand protein belonging to the CREC family. As other members of the family, it is localized in the secretory pathway and regulates the activity of SERCA2a and of the ryanodine receptor in the endoplasmic reticulum (ER). We have studied the effects of Ca2+ binding to the protein and found it to attain a more compact structure upon ion binding. Circular Dichroism (CD) measurements suggest a major rearrangement of the protein secondary structure, which reversibly switches from disordered at low Ca2+ concentrations to predominantly alpha-helical when Ca2+ is added. SAXS experiments confirm the transition from an unfolded to a compact structure, which matches the structural prediction of a trilobal fold. Overall our experiments suggest that calumenin is a Ca2+ sensor, which folds into a compact structure, capable of interacting with its molecular partners, when Ca2+ concentration within the ER reaches the millimolar range. PMID:26991433

  15. CaMKII in the Cardiovascular System: Sensing Redox States

    Science.gov (United States)

    Erickson, Jeffrey R.; He, B. Julie; Grumbach, Isabella M.; Anderson, Mark E

    2013-01-01

    The multifunctional Ca2+ and calmodulin-dependent protein kinase II (CaMKII) is now recognized to play a central role in pathological events in the cardiovascular system. CaMKII has diverse downstream targets that promote vascular disease, heart failure and arrhythmias, so improved understanding of CaMKII signaling has the potential to lead to new therapies for cardiovascular disease. CaMKII is a multimeric serine-threonine kinase that is initially activated by binding calcified calmodulin (Ca2+/CaM). Under conditions of sustained exposure to elevated Ca2+/CaM CaMKII transitions into a Ca2+/CaM-autonomous enzyme by two distinct but parallel processes. Autophosphorylation of threonine 287 in the CaMKII regulatory domain ‘traps’ CaMKII into an open configuration even after Ca2+/CaM unbinding. More recently, our group identified a pair of methionines (281/282) in the CaMKII regulatory domain that undergo a partially reversible oxidation which, like autophosphorylation, prevents CaMKII from inactivating after Ca2+/CaM unbinding. Here we review roles of CaMKII in cardiovascular disease with an eye to understanding how CaMKII may act as a transduction signal to connect pro-oxidant conditions into specific downstream pathological effects that are relevant to rare and common forms of cardiovascular disease. PMID:21742790

  16. Local mechanical and electromechanical properties of the P(VDF-TrFE)-graphene oxide thin films

    Science.gov (United States)

    Silibin, M. V.; Bystrov, V. S.; Karpinsky, D. V.; Nasani, N.; Goncalves, G.; Gavrilin, I. M.; Solnyshkin, A. V.; Marques, P. A. A. P.; Singh, Budhendra; Bdikin, I. K.

    2017-11-01

    Recently, many organic materials, including carbon materials such as carbon nanotubes (CNTs) and graphene (single-walled carbon sheet structure) were studied in order to improve their mechanical and electrical properties. In particular, copolymers of poly (vinylidene fluoride) and poly trifluoroethylene [P(VDF-TrFE)] are promising materials, which can be used as probes, sensors, actuators, etc. Composite thin film of the copolymer P(VDF-TrFE) with graphene oxide (GO) were prepared by spin coating. The obtained films were investigated using piezoresponse force microscopy (PFM). The switching behavior, piezoelectric response, dielectric permittivity and mechanical properties of the films were found to depend on the presence of GO. For understanding the mechanism of piezoresponse evolution of the composite we used models of PVDF chain, its behavior in electrical field and computed the data for piezoelectric coefficients using HyperChem software. The summarized models of graphene oxide based on graphene layer from 96 carbon atoms C: with oxygen and OH groups and with COOH groups arranged by hydrogen were used for PVDF/Graphene oxide complex: 1) with H-side (hydrogen atom) connected from PVDF to graphene oxide, 2) with F-side (fluorine atom) connected from PVDF graphene oxide and 3) Graphene Oxide/PVDF with both sides (sandwich type). Experimental results qualitatively correlate with those obtained in the calculations.

  17. The Present and Future Statues of Animal Presence in Turkey; The Declination of TR83 Area

    Directory of Open Access Journals (Sweden)

    Gülistan Erdal

    2016-08-01

    Full Text Available In this study, the cattle and small cattle presence in Turkey and in the area of TR83 which consists of Amasya, Çorum, Samsun and Tokat provinces were analysed. Trend analysis were carried out using the data obtained from cattle and small cattle presence with the help of the values of the period of 1996 – 2014 and estimated values for 2020 were specified. It is seen in the projections for the 2020 that, domestic cattle breeds are expected to be decreased in number whereas it is expected to be increased in the TR83 area. Also it is estimated that the number of the whole animal existence which are investigated by the study will increase by the year 2020. It is beyond question that the positive effects of the present policies supporting stockbreeding on this increase is apparent. In this sense, the determination of the contribution of present stockbreeding support policies to the sector and their effectiveness and the investigation of their faulting aspects are important in order to develop more accurate and effective policies.

  18. Serial correlations in single-subject fMRI with sub-second TR.

    Science.gov (United States)

    Bollmann, Saskia; Puckett, Alexander M; Cunnington, Ross; Barth, Markus

    2017-10-21

    When performing statistical analysis of single-subject fMRI data, serial correlations need to be taken into account to allow for valid inference. Otherwise, the variability in the parameter estimates might be under-estimated resulting in increased false-positive rates. Serial correlations in fMRI data are commonly characterized in terms of a first-order autoregressive (AR) process and then removed via pre-whitening. The required noise model for the pre-whitening depends on a number of parameters, particularly the repetition time (TR). Here we investigate how the sub-second temporal resolution provided by simultaneous multislice (SMS) imaging changes the noise structure in fMRI time series. We fit a higher-order AR model and then estimate the optimal AR model order for a sequence with a TR of less than 600 ms providing whole brain coverage. We show that physiological noise modelling successfully reduces the required AR model order, but remaining serial correlations necessitate an advanced noise model. We conclude that commonly used noise models, such as the AR(1) model, are inadequate for modelling serial correlations in fMRI using sub-second TRs. Rather, physiological noise modelling in combination with advanced pre-whitening schemes enable valid inference in single-subject analysis using fast fMRI sequences. Copyright © 2017 Elsevier Inc. All rights reserved.

  19. Digital Calibration of TR Modules for Real-time Digital Beamforming SweepSAR Architectures

    Science.gov (United States)

    Hoffman, James Patrick; Perkovic, Dragana; Shaffer, Scott; Veilleux, Louise; Peral, Eva

    2011-01-01

    Real-time digital beamforming, combined with lightweight, large aperture reflectors, enable SweepSAR architectures such as that of the proposed DESDynI [Deformation, Ecosystem Structure, and Dynamics of Ice] SAR [Synthetic Aperture Radar] Instrument (or DSI). These new instrument concepts require new methods for calibrating the multiple channels, which must be combined on-board, in real-time. The calibration of current state-of-the-art Electronically Steered Arrays typically involves pre-flight TR (Transmit/Receive) module characterization over temperature, and in-flight correction based on temperature, which ignores the effects of element aging and drifts unrelated to temperature. We are developing new methods for digitally calibrating digital beamforming arrays to reduce development time, risk and cost of precision calibrated TR modules for array architectures by accurately tracking modules' characteristics through closed-loop Digital Calibration, thus tracking systematic changes regardless of temperature. The benefit of this effort is that it would enable a new class of lightweight radar architecture, Digital Beamforming with SweepSAR, providing significantly larger swath coverage than conventional SAR architectures for solid earth and biomass remote sensing, while reducing mission mass and cost. This new instrument concept requires new methods for calibrating the multiple channels, which must be combined on-board, in real-time.

  20. Digital Calibration of TR Modules for Real-Tme Digital Beamforming SweepSAR Architectures

    Science.gov (United States)

    Hoffman, James Patrick; Perkovic, Dragana; Shaffer, Scott; Veilleux, Louise; Peral, Eva

    2012-01-01

    Real-time digital beamforming, combined with lightweight, large aperture reflectors, enable a new architecture, which is the baseline for the proposed DESDynI [Deformation, Ecosystem Structure, and Dynamics of Ice] SAR [Synthetic Aperture Radar] Instrument (or DSI). This new instrument concept requires new methods for calibrating multiple simultaneous channels. The calibration of current state-of-the-art Electronically Steered Arrays typically involves pre-flight TR (Transmit/Receive) module characterization over temperature, and in-flight correction based on measured temperatures. This method ignores the effects of element aging and any drifts unrelated to temperature. We are developing new digital calibration of digital beamforming arrays, which helps to reduce development time, risk and cost. Precision calibrated TR modules enable real-time beamforming architectures by accurately tracking modules' characteristics through closed-loop digital calibration, which tracks systematic changes regardless of temperature. The benefit of this effort is that it would enable a new, lightweight radar architecture, with on-board digital beamforming. This provides significantly larger swath coverage than conventional SAR architectures

  1. SoilTrEC: a global initiative on critical zone research and integration.

    Science.gov (United States)

    Menon, Manoj; Rousseva, Svetla; Nikolaidis, Nikolaos P; van Gaans, Pauline; Panagos, Panos; de Souza, Danielle Maia; Ragnarsdottir, Kristin Vala; Lair, Georg J; Weng, Liping; Bloem, Jaap; Kram, Pavel; Novak, Martin; Davidsdottir, Brynhildur; Gisladottir, Gudrun; Robinson, David A; Reynolds, Brian; White, Tim; Lundin, Lars; Zhang, Bin; Duffy, Christopher; Bernasconi, Stefano M; de Ruiter, Peter; Blum, Winfried E H; Banwart, Steven A

    2014-02-01

    Soil is a complex natural resource that is considered non-renewable in policy frameworks, and it plays a key role in maintaining a variety of ecosystem services (ES) and life-sustaining material cycles within the Earth's Critical Zone (CZ). However, currently, the ability of soil to deliver these services is being drastically reduced in many locations, and global loss of soil ecosystem services is estimated to increase each year as a result of many different threats, such as erosion and soil carbon loss. The European Union Thematic Strategy for Soil Protection alerts policy makers of the need to protect soil and proposes measures to mitigate soil degradation. In this context, the European Commission-funded research project on Soil Transformations in European Catchments (SoilTrEC) aims to quantify the processes that deliver soil ecosystem services in the Earth's Critical Zone and to quantify the impacts of environmental change on key soil functions. This is achieved by integrating the research results into decision-support tools and applying methods of economic valuation to soil ecosystem services. In this paper, we provide an overview of the SoilTrEC project, its organization, partnerships and implementation.

  2. Diagnosis and management of the venous malformations of Klippel-Trénaunay syndrome.

    Science.gov (United States)

    Wang, S Keisin; Drucker, Natalie A; Gupta, Alok K; Marshalleck, Francis E; Dalsing, Michael C

    2017-07-01

    A dearth of information exists in the literature regarding current practice in the management of Klippel-Trénaunay syndrome (KTS), a rare condition. We review and describe the etiology, diagnosis, and treatment of KTS. Relevant data were synthesized from a Medline review using a combination of the keyterms "Klippel" and "Trénaunay." The majority of hits described singular case reports and were subsequently excluded. The remaining papers were then reviewed and included on the basis of the quality of evidence and the authors' discretion. KTS is characterized by a clinical triad of extremity varicosities, cutaneous vascular malformations, and hypertrophy of soft tissues and long bones. The diagnosis is clinically supplemented with magnetic resonance imaging and computed tomography. Although this syndrome is associated with significant comorbidities, such as pain, edema, ulcerations, and pruritus, it is rarely the cause of death. The backbone of treatment is nonoperative in nature but should be supplemented with minimally invasive, endovascular, and rarely open surgical procedures for refractory cases. Published by Elsevier Inc.

  3. Synthesis, physical and microwave absorption properties of Barium ferrite - P(VDF-TrFE) nanocomposites

    Science.gov (United States)

    Gutiérrez, Jon; Miren Gutiérrez Muto, Ane; Peña, Alazne; San Sebastián, Maria; Catarina Lopes, Ana; Lezama, Luis; Gil de Muro, Izaskun; Larrañaga, A.; Orue, I.

    2017-12-01

    We present results concerning the synthesis, magnetic, dielectric and microwave absorption properties of BaFe12O19 / P(VDF-TrFE) nanocomposites. First, barium ferrite nanopowders were prepared by the hydrothermal synthesis method. Structural and morphological properties of the prepared powders were investigated by X-ray diffraction (XRD) and transmission electron microscopy (TEM), showing as result a good quality spherical plus platelet-like shaped nanoparticles. Afterwards, composite films with filler nanoparticles of barium ferrite BaFe12O19 (BaFO, nominal 5-20 wt.%) dispersed within P(VDF-TrFE) acting as polymeric matrix have been prepared by solvent evaporation. Magnetic properties were examined by the vibrating sample magnetometry (VSM), and a direct comparison of results obtained for the composites respect to the pure nanopowder allow us to obtain the true nanofiller content value, different from the initially nominal one. Dielectric properties have been measured up to 2 MHz and show the typical behaviour of Maxwell-Wagner type interfacial polarization. From 10 kHz up, permittivity of the composites remains almost unchanged, with values that show a smooth increase as the %wt. of BaFe12O19 does. Finally, microwave absorption properties were analyzed by using ESR technique operating at X-band. All obtained results are discussed in terms of size and quantity of the composites BaFe12O19 filler nanoparticles.

  4. QoS Provisioning for TT-TR Based OBS Ring Networks

    Science.gov (United States)

    Peng, Limei; Kim, Young-Chon; Tang, Wan; Youn, Chan-Hyun

    2011-06-01

    Optical burst switching (OBS) is one of the most promising switching technologies. This paper studies the efficient OBS ring network using the TT-TR based node architecture. The TT-TR based OBS ring network can use the network resources very efficiently, but the network performance is restricted due to the limited network resources. Moreover, different network demands have different network resource requirements. It is very important to satisfy their different requirements while using the limited network resources efficiently. In this paper, Quality of Service (QoS) provisioning mechanisms are studied to solve the above mentioned problems. Two kinds of network services, namely the real-time traffic and the best-effort traffic, are considered in this paper. The real-time service is delay-sensitive that should be transmitted in a real-time manner and cannot tolerate high transmission delay; while the best-effort traffic has no strict limitation in transmission delay or loss, but the best effort are made to improve their transmission reliability. According to their different servicing requirements, different QoS provisioning mechanisms are proposed to serve them in the OBS unidirectional and bidirectional ring networks, respectively. The proposed mechanisms are evaluated and analyzed through simulation. The simulation results show that even though the proposed mechanisms cannot get absolute zero-delay or zero-loss for real-time traffic or best-effort traffic, their different requirements for delay or loss are satisfied to some significant degree.

  5. A viagem de Lévi-Strauss aos trópicos

    Directory of Open Access Journals (Sweden)

    Silviano Santiago

    2001-03-01

    Full Text Available O ódio à viagem e aos exploradores, confessado por Claude Lévi-Strauss desde a abertura de Tristes trópicos, fundamenta a ambivalência do conceito de etnocentrismo na sua teoria etnográfica. Pureza e distância original entre culturas diferentes delimitam o campo da análise feita pelo autor. No entanto, graças a um golpe do acaso, como ele também confessa, sua viagem se realiza. A presença intelectual de Lévi-Strauss na formação da USP, na década de 30, fundamenta uma faseoutra da discussão sobre o transplante das idéias progressistas européias para os trópicos, cujo rebento mais recente é a polêmica entre Maria Sylvia de Carvalho Franco e Roberto Schwarz sobre as idéias “fora do lugar”.

  6. NORE1A Regulates MDM2 Via β-TrCP

    Directory of Open Access Journals (Sweden)

    M. Lee Schmidt

    2016-03-01

    Full Text Available Mouse Double Minute 2 Homolog (MDM2 is a key negative regulator of the master tumor suppressor p53. MDM2 regulates p53 on multiple levels, including acting as an ubiquitin ligase for the protein, thereby promoting its degradation by the proteasome. MDM2 is oncogenic and is frequently found to be over-expressed in human tumors, suggesting its dysregulation plays an important role in human cancers. We have recently found that the Ras effector and RASSF (Ras Association Domain Family family member RASSF5/NORE1A enhances the levels of nuclear p53. We have also found that NORE1A (Novel Ras Effector 1A binds the substrate recognition component of the SCF-ubiquitin ligase complex β-TrCP. Here, we now show that NORE1A regulates MDM2 protein levels by targeting it for ubiquitination by SCF-β-TrCP. We also show the suppression of NORE1A protein levels enhances MDM2 protein expression. Finally, we show that MDM2 can suppress the potent senescence phenotype induced by NORE1A over-expression. Thus, we identify a mechanism by which Ras/NORE1A can modulate p53 protein levels. As MDM2 has several important targets in addition to p53, this finding has broad implications for cancer biology in tumor cells that have lost expression of NORE1A due to promoter methylation.

  7. Detection of fast oscillating magnetic fields using dynamic multiple TR imaging and Fourier analysis.

    Directory of Open Access Journals (Sweden)

    Ki Hwan Kim

    Full Text Available Neuronal oscillations produce oscillating magnetic fields. There have been trials to detect neuronal oscillations using MRI, but the detectability in in vivo is still in debate. Major obstacles to detecting neuronal oscillations are (i weak amplitudes, (ii fast oscillations, which are faster than MRI temporal resolution, and (iii random frequencies and on/off intervals. In this study, we proposed a new approach for direct detection of weak and fast oscillating magnetic fields. The approach consists of (i dynamic acquisitions using multiple times to repeats (TRs and (ii an expanded frequency spectral analysis. Gradient echo echo-planar imaging was used to test the feasibility of the proposed approach with a phantom generating oscillating magnetic fields with various frequencies and amplitudes and random on/off intervals. The results showed that the proposed approach could precisely detect the weak and fast oscillating magnetic fields with random frequencies and on/off intervals. Complex and phase spectra showed reliable signals, while no meaningful signals were observed in magnitude spectra. A two-TR approach provided an absolute frequency spectrum above Nyquist sampling frequency pixel by pixel with no a priori target frequency information. The proposed dynamic multiple-TR imaging and Fourier analysis are promising for direct detection of neuronal oscillations and potentially applicable to any pulse sequences.

  8. β-TrCP1 Is a Vacillatory Regulator of Wnt Signaling.

    Science.gov (United States)

    Long, Marcus John; Lin, Hong-Yu; Parvez, Saba; Zhao, Yi; Poganik, Jesse Richard; Huang, Paul; Aye, Yimon

    2017-08-17

    Simultaneous hyperactivation of Wnt and antioxidant response (AR) are often observed during oncogenesis. However, it remains unclear how the β-catenin-driven Wnt and the Nrf2-driven AR mutually regulate each other. The situation is compounded because many players in these two pathways are redox sensors, rendering bolus redox signal-dosing methods uninformative. Herein we examine the ramifications of single-protein target-specific AR upregulation in various knockdown lines. Our data document that Nrf2/AR strongly inhibits β-catenin/Wnt. The magnitude and mechanism of this negative regulation are dependent on the direct interaction between β-catenin N terminus and β-TrCP1 (an antagonist of both Nrf2 and β-catenin), and independent of binding between Nrf2 and β-TrCP1. Intriguingly, β-catenin positively regulates AR. Because AR is a negative regulator of Wnt regardless of β-catenin N terminus, this switch of function is likely sufficient to establish a new Wnt/AR equilibrium during tumorigenesis. Copyright © 2017 Elsevier Ltd. All rights reserved.

  9. Experimental Analysis Of 80 Tr Capacity Air Cooled Scroll Chiller Using R-22 & R-407c.

    Directory of Open Access Journals (Sweden)

    Mr. Bhikhu B,

    2014-04-01

    Full Text Available In air conditioning systems, chilled water is typically distributed to heat exchangers, or coils, in air handling units or other types of terminal devices which cool the air in their respective space(s, and then the water is recirculated back to the chiller to be cooled again. These cooling coils transfer sensible heat and latent heat from the air to the chilled water, thus cooling and usually dehumidifying the air stream. The experiment works on 80 TR capacity Air Cooled Scroll Chiller systems. Vapour compression refrigeration cycle is used for cooling chilling water. Capacity of compressor is taken same for the different refrigerants used for experimental analysis. During experimental work used R-22 and R-407C as refrigerants. Theoretical COP of system with R- 22 refrigerant is 4.166 and actual COP is 2.227. For 80 TR capacity scroll air cooled chiller, theoretical COP of system with R-407c refrigerant is 3.465 and actual COP is 2.745 respectively. Based on the result analysis the same capacity of air cooling system with scroll compressor Actual COP of R-407C is higher than R-22. It means R-407C is also a alternative refrigerant for air cooled chilling system and also for HVAC system.

  10. Dom casmurro - romance, trágico, romântico ou realista

    Directory of Open Access Journals (Sweden)

    Kathrin H. Rosenfield

    2001-03-01

    Full Text Available Dom Casmurro é freqüentemente apresentado como um romance que desenvolve a temática da infidelidade. Outras abordagens otratam como um romance “trágico”, comparável a Em busca do tempo perdido, porém demunido do consolo metafísico que fornece a obra deProust. Propomos aqui uma leitura que mostra como Machado se inscreve na dissolução das estruturas trágicas, segundo a tradição narrativa de Flaubert e de Maupassant. Em vez de focalizar um “antes” idílico, idade de ouro perdida “depois” da descoberta da suposta infidelidade, procuramos mostrar que o “Mal” machadiano se prepara nas constelações da timidez, da moleza dos sentidos e do espírito. Nessa perspectiva, Dom Casmurro é uma versão moderna da temida acedia melancólica e Machado desenha, com um misto de ironia e de ternura, a fisionomía moderna do vício que Dürer fixou na sua famosa litografia “A Melancolia”. No acanhamento tímido desaparece a transgressão positiva ou passional, e no lugar de uma ação fatal surge a entrega passiva ao que é dado – o gosto indulgente da repetição e o ressentimento.

  11. Amyloid formation and disaggregation of {alpha}-synuclein and its tandem repeat ({alpha}-TR)

    Energy Technology Data Exchange (ETDEWEB)

    Bae, Song Yi; Kim, Seulgi; Hwang, Heejin; Kim, Hyun-Kyung; Yoon, Hyun C.; Kim, Jae Ho [Department of Molecular Science and Technology, Graduate School of Interdisciplinary Programs, Ajou University, Suwon 443-749 (Korea, Republic of); Lee, SangYoon, E-mail: sangyoon@ajou.ac.kr [Chronic Inflammatory Disease Research Center, School of Medicine, Ajou University, Suwon 443-749 (Korea, Republic of); Kim, T. Doohun, E-mail: doohunkim@ajou.ac.kr [Department of Molecular Science and Technology, Graduate School of Interdisciplinary Programs, Ajou University, Suwon 443-749 (Korea, Republic of)

    2010-10-01

    Research highlights: {yields} Formation of the {alpha}-synuclein amyloid fibrils by [BIMbF{sub 3}Im]. {yields} Disaggregation of amyloid fibrils by epigallocatechin gallate (EGCG) and baicalein. {yields} Amyloid formation of {alpha}-synuclein tandem repeat ({alpha}-TR). -- Abstract: The aggregation of {alpha}-synuclein is clearly related to the pathogenesis of Parkinson's disease. Therefore, detailed understanding of the mechanism of fibril formation is highly valuable for the development of clinical treatment and also of the diagnostic tools. Here, we have investigated the interaction of {alpha}-synuclein with ionic liquids by using several biochemical techniques including Thioflavin T assays and transmission electron microscopy (TEM). Our data shows a rapid formation of {alpha}-synuclein amyloid fibrils was stimulated by 1-butyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide [BIMbF{sub 3}Im], and these fibrils could be disaggregated by polyphenols such as epigallocatechin gallate (EGCG) and baicalein. Furthermore, the effect of [BIMbF{sub 3}Im] on the {alpha}-synuclein tandem repeat ({alpha}-TR) in the aggregation process was studied.

  12. Immortalization of chicken preadipocytes by retroviral transduction of chicken TERT and TR.

    Directory of Open Access Journals (Sweden)

    Wei Wang

    Full Text Available The chicken is an important agricultural animal and model for developmental biology, immunology and virology. Excess fat accumulation continues to be a serious problem for the chicken industry. However, chicken adipogenesis and obesity have not been well investigated, because no chicken preadipocyte cell lines have been generated thus far. Here, we successfully generated two immortalized chicken preadipocyte cell lines through transduction of either chicken telomerase reverse transcriptase (chTERT alone or in combination with chicken telomerase RNA (chTR. Both of these cell lines have survived >100 population doublings in vitro, display high telomerase activity and have no sign of replicative senescence. Similar to primary chicken preadipocytes, these two cell lines display a fibroblast-like morphology, retain the capacity to differentiate into adipocytes, and do not display any signs of malignant transformation. Isoenzyme analysis and PCR-based analysis confirmed that these two cell lines are of chicken origin and are free from inter-species contamination. To our knowledge, this is the first report demonstrating the generation of immortal chicken cells by introduction of chTERT and chTR. Our established chicken preadipocyte cell lines show great promise as an in vitro model for the investigation of chicken adipogenesis, lipid metabolism, and obesity and its related diseases, and our results also provide clues for immortalizing other avian cell types.

  13. Sporadic Ca and Ca+ layers at mid-latitudes: Simultaneous observations and implications for their formation

    Directory of Open Access Journals (Sweden)

    M. Gerding

    2001-01-01

    Full Text Available We report on the observations of 188 sporadic layers of either Ca atoms and/or Ca ions that we have observed during 112 nights of lidar soundings of Ca, and 58 nights of Ca+ soundings, at Kühlungsborn, Germany (54° N, 12° E. The Ca+ soundings have been performed simultaneously and in a common volume with the Ca soundings by two separate lidars. Correlations between sporadic neutral and ionized metal layers are demonstrated through four case studies. A systematic study of the variations of occurrence of sporadic Ca and Ca+ layers reveals that neutral and ionized Ca layers are not as closely correlated as expected earlier: (a The altitude distribution shows the simultaneous occurrence of both sporadic Ca and Ca+ layers to be most likely only in the narrow altitude range between 90 and 95 km. Above that region, in the lower thermosphere, the sporadic ion layers are much more frequent than atom layers. Below 90 km only very few sporadic layers have been observed; (b The seasonal variation of sporadic Ca layers exhibits a minimum of occurrence in summer, while sporadic Ca+ layers do not show a significant seasonal variation (only the dense Ca+ layers appear to have a maximum in summer. At mid-latitudes sporadic Ca layers are more frequent than sporadic layers of other atmospheric metals like Na or K. For the explanation of our observations new formation mechanisms are discussed.Key words. Ionosphere (ion chemistry and composition; ionosphere-atmosphere interactions; mid-latitude ionosphere

  14. Results of measuring internal Ca absorption and Ca kinetics with /sup 47/Ca in patients suffering from chronically recurrent urolithiasis

    Energy Technology Data Exchange (ETDEWEB)

    Hoffmann, L.; Esther, G.; Serfling, D.; Bast, R. (Rostock Univ. (German Democratic Republic). Radiologische Klinik)

    1983-07-01

    47 patients with chronically recurrent calcium lithiasis (with between 3 and over 100 stone episodes) and an average age of 43.2 years have been examined. The Ca absorption lay between 42.7% and 90.0% of the dose, the average was 59.3% with a standard deviation of +- 12.9. This is higher than in persons with healthy kidneys. The Ca absorption is significantly lower in patients with renal insufficiency. Studies on Ca kinetics revealed turnover rates and pool sizes within the normal range.

  15. Influência de três modos de fotopolimerização sobre a microdureza de três resinas compostas

    Directory of Open Access Journals (Sweden)

    Andréa Cristina Schneider

    2016-01-01

    Full Text Available Resumo A adequada fotopolimerização das resinas compostas é fundamental para obtenção de uma boa dureza capaz de resistir aos esforços mastigatórios. Assim, o objetivo deste estudo foi avaliar a influência das técnicas de fotopolimerização - Convencional (CONV, Soft-start (SS e Pulso atrasado (PA na microdureza Knoop de três resinas compostas. Para a confecção dos corpos-de-prova foram utilizadas as resinas compostas Filtek Z350 (3M ESPE, Empress Direct (IvoclarVivadent e P90 (3M ESPE. Foram obtidos nove grupos experimentais em função da técnica de polimerização e resina composta. Vinte e quatro horas após a fotopolimerização foi realizado o teste de microdureza Knoop nas superfícies de base e topo de cada corpo-de-prova. Assim, os valores obtidos foram submetidos ao teste de Kruskall Wallis, seguido do teste de Dunn, p < 0,05. A eficácia da polimerização na superfície de topo das resinas compostas testadas não foi afetada pelos diferentes modos de polimerização. A resina composta Empress Direct apresentou os mais baixos valores de dureza para todos os modos de polimerização quando comparada às demais resinas compostas avaliadas.

  16. Ischemic damage in hippocampal CA1 is dependent on glutamate release and intact innervation from CA3

    DEFF Research Database (Denmark)

    Benveniste, H; Jørgensen, M B; Sandberg, M

    1989-01-01

    The removal of glutamatergic afferents to CA1 by destruction of the CA3 region is known to protect CA1 pyramidal cells against 10 min of transient global ischemia. To investigate further the pathogenetic significance of glutamate, we measured the release of glutamate in intact and CA3-lesioned CA1...... hippocampal tissue. In intact CA1 hippocampal tissue, glutamate increased sixfold during ischemia; in the CA3-lesioned CA1 region, however, glutamate only increased 1.4-fold during ischemia. To assess the neurotoxic potential of the ischemia-induced release of glutamate, we injected the same concentration...... of glutamate into the CA1 region as is released during ischemia in normal, CA3-lesioned, and ischemic CA1 tissue. We found that this particular concentration of glutamate was sufficient to destroy CA1 pyramids in the vicinity of the injection site in intact and CA3-lesioned CA1 tissue when administered during...

  17. Superconductivity in Ca-intercalated bilayer graphene

    Science.gov (United States)

    Mazin, I. I.; Balatsky, A. V.

    2010-10-01

    Recent observation of proximity effect [H.B. Heersche, P. Jarillo-Herrero, J.B. Oostinga, L.M.K. Vandersypen, and A.F. Morpurgo, Nature, bf 446 (2007) p. 05555.] has ignited interest in superconductivity in graphene and its derivatives. We consider Ca-intercalated graphene bilayer and argue that it is a superconductor, and likely with a sizeable T c . We find substantial and suggestive similarities between Ca-intercalated bilayer (C6CaC6), and CaC6, an established superconductor with T c = 11.5 K. In particular, the nearly free electron band, proven to be instrumental for superconductivity in intercalated graphites, does cross the chemical potential in (C6CaC6), despite the twice smaller doping level, satisfying the so-called "Cambridge criterion". Calculated properties of zone-center phonons are very similar to those of CaC6. This suggests that the critical temperature would probably be on the same scale as in CaC6.

  18. Juvenile Hippocampal CA2 Region Expresses Aggrecan

    Directory of Open Access Journals (Sweden)

    Asako Noguchi

    2017-05-01

    Full Text Available Perineuronal nets (PNNs are distributed primarily around inhibitory interneurons in the hippocampus, such as parvalbumin-positive interneurons. PNNs are also present around excitatory neurons in some brain regions and prevent plasticity in these neurons. A recent study demonstrated that PNNs also exist around mouse hippocampal pyramidal cells, which are the principle type of excitatory neurons, in the CA2 subregion and modulate the excitability and plasticity of these neurons. However, the development of PNNs in the CA2 region during postnatal maturation was not fully investigated. This study found that a main component of PNNs, aggrecan, existed in the pyramidal cell layer of the putative CA2 subarea prior to the appearance of the CA2 region, which was defined by the CA2 marker protein regulator of G protein signaling 14 (RGS14. We also found that aggrecan immunoreactivity was more evident in the anterior sections of the CA2 area than the posterior sections, which suggests that the function of CA2 PNNs varies along the anterior-posterior axis.

  19. Inhibition of CaMKK β and CaMK IV is detrimental in cerebral ischemia

    Science.gov (United States)

    McCullough, Louise D.; Tarabishy, Sami; Benashski, Sharon; Xu, Yan; Ribar, Thomas; Means, Anthony; Li, Jun

    2013-01-01

    Background and Purpose Elevation of intracellular calcium was traditionally thought to be detrimental in stroke pathology. However, clinical trials testing treatments which block calcium signaling have failed to improve outcomes in ischemic stroke. Emerging data suggest that calcium may also trigger endogenous protective pathways following stroke. CaMKK (calcium/calmodulin-dependent protein kinase kinase) is a major kinase activated by rising intracellular calcium. Compelling evidence has suggested that CaMKK and its downstream kinase CaMK IV are critical in neuronal survival when cells are under ischemic stress. We examined the functional role of CaMKK/CaMK IV signaling in stroke. Methods We utilized middle cerebral artery occlusion model in mice. Results Our data demonstrated that pharmacological and genetic inhibition of CaMKK aggravated stroke injury. Additionally, deletion of CaMKK β, one of the two CaMKK isoforms, reduced CaMK IV activation and CaMK IV deletion in mice worsened stroke outcome. Finally, CaMKK β or CaMK IV KO mice had exacerbated BBB (blood brain barrier) disruption evidenced by increased hemorrhagic transformation rates and activation of matrix metalloproteinase. We observed transcriptional inactivation including reduced levels of BCL2 (B-cell lymphoma 2) and HDAC4 (histone deacetylase 4) phosphorylation in those KO mice after stroke. Conclusions Our data has established that the CaMKK/CaMK IV pathway is a key endogenous protective mechanism in ischemia. Our results suggest that this pathway serves as important regulator of BBB integrity and transcriptional activation of neuroprotective molecules in stroke. PMID:23868268

  20. Variability among research diagnostic interview instruments in the application of DSM-IV-TR criteria for pediatric bipolar disorder.

    Science.gov (United States)

    Galanter, Cathryn A; Hundt, Stephanie R; Goyal, Parag; Le, Jenna; Fisher, Prudence W

    2012-06-01

    The DSM-IV-TR criteria for a manic episode and bipolar disorder (BD) were developed for adults but are used for children. The manner in which clinicians and researchers interpret these criteria may have contributed to the increase in BD diagnoses given to youth. Research interviews are designed to improve diagnostic reliability and validity, but vary in how they incorporate DSM-IV-TR criteria for pediatric BD. We examined DSM-IV-TR criteria and the descriptive text for a manic episode and the mania sections of six commonly used pediatric diagnostic research interviews focusing on the following: interpretation of DSM-IV-TR, recommendations for administration, and scoring methods. There are differences between the DSM-IV-TR manic episode criteria and descriptive text. Instruments vary in several ways including in their conceptualization of the mood criterion, whether symptoms must represent a change from the child's usual state, and whether B-criteria are required to co-occur with the A-criterion. Instruments also differ on recommendations for administration and scoring methods. Given the differences between DSM-IV-TR manic episode criteria and explanatory text, it is not surprising that there is considerable variation between diagnostic instruments based on DSM-IV-TR. These differences likely lead to dissimilarities in subjects included in BD research studies and inconsistent findings across studies. The field of child psychiatry would benefit from more uniform methods of assessing symptoms and determining pediatric BD diagnoses. We discuss recommendations for changes to future instruments, interviews, assessment, and the DSM-5. Copyright © 2012 American Academy of Child and Adolescent Psychiatry. Published by Elsevier Inc. All rights reserved.

  1. Jovens no trânsito: do sentido do dirigir ao desenvolvimento de consciência

    Directory of Open Access Journals (Sweden)

    Sonia Chébel Mercado Sparti

    2008-12-01

    Full Text Available Introdução: a complexa situação relativa à circulação humana tem suscitado muitas indagações. O advento do automóvel, ao lado das dimensões positivas, também trouxe consigo um conjunto de graves problemas, destacando-se pela sua magnitude e importância, os acidentes de trânsito. Objetivo: esta pesquisa buscou conhecer como a reflexão sobre o sentido do dirigir possibilita o desenvolvimento de consciência de jovens universitários/as. Metodologia: foram utilizadas duas Entrevistas Reflexivas, realizadas de maneira Coletiva, com dez universitários/as da PUC-São Paulo/campus Sorocaba, seguidas de devolução (com pré-análise dos dados. A opção pela Entrevista Reflexiva deveu-se, dentre outros fatores, ao seu caráter de intervenção, uma vez que a escuta atenta e respeitosa pode ser um momento de ajuda. A Grounded Theory foi utilizada para a organização dos dados. Resultados: este procedimento reflexivo possibilitou a compreensão dos aspectos positivos e negativos relativos à circulação humana e levantou fatores indicativos de prevenção: afirmações categóricas a respeito das próprias habilidades ao volante cederam lugar ao reconhecimento de que ainda há espaço para seu aperfeiçoamento; o olhar relativo ao outro foi se modificando, de maneira a considerá-lo parceiro, e não adversário; a preservação da vida sendo considerada um valor, em direção a um que fazer mais prudente, no espaço compartilhado do trânsito. Conclusão: estes resultados ratificam a proposta de que é possível (e desejável pensar a educação para o trânsito na perspectiva do desenvolvimento de consciência.

  2. O enfrentamento ao tráfico de pessoas no âmbito do Mercosul

    Directory of Open Access Journals (Sweden)

    Mário Lúcio Quintão Soares

    2011-12-01

    Full Text Available Human trafficking is a serious problemthat, in its various forms – sexual exploitation,forced labor and removal of organs – isin the form of transnational organized crime,affecting every country in the world. Apparently,the increasing political integration, economicand social activity is one way to attempt to minimizethe human trafficking. In the case of theSouthern Cone Common Market – Mercosur,efforts have been made to both by its States Parties(Argentina, Brazil, Paraguay and Uruguay,as they ratified the Palermo Protocol. Within thisframework, this study aimed to examine the measuresadopted within Mercosur in a way to protectand promote the rights of trafficked persons.To accomplish this work, the emphasis has beendescriptive and analytical study, by means of literatureand documents. Note that only Brazil andArgentina have adopted specific policies on traffickingin persons. Given this, it was found thatthe States Parties of Mercosur develop actions toprotect and promote human rights of traffickedpersons, not counting even some specific publicpolicy.O tráfico de pessoas é um problema graveque, nas suas diversas modalidades – exploraçãosexual, trabalhos forçados e remoção de órgãos –constitui-se em forma de crime organizado transnacional,atingindo todos os países do mundo. Ao queparece, a crescente integração política, econômica esocial constitui caminho para uma tentativa de minimizaçãodo tráfico de pessoas. Em se tratando deMercado Comum do Cone Sul – Mercosul, esforçostêm sido envidados para tanto por parte dos seus EstadosPartes (Argentina, Brasil, Paraguai e Uruguai,pois esses países ratificaram o Protocolo de Palermo.Nesse marco, esta pesquisa teve por objetivo analisaras medidas adotadas no âmbito do Mercosul para aproteção e promoção dos direitos das pessoas traficadas.Para a concretização deste trabalho, privilegiou-se o estudo descritivo e analítico, por meio depesquisa bibliográfica e

  3. Indsatsen over for børn med handicap og træningsbehov

    DEFF Research Database (Denmark)

    Wüst, Miriam; Thorsager, Linda; Bengtsson, Steen

    Formålet med denne rapport er at kortlægge den offentlige trænings- og behandlingsindsats over for børn med handicap i førskolealderen, herunder at undersøge forældrenes tilfredshed med indsatsen. Undersøgelsen bygger på en spørgeskemaundersøgelse foretaget i 2007 blandt forældre, fagpersoner i...... daginstitutioner og kommunale sagsbehandlere til i alt 317 børn med handicap. Børnene er alle født i 2001/2002 og har modtaget eller modtager en offentlig hjælpeindsats i forbindelse med deres handicap. Rapporten inddrager endvidere fokusgruppeinterview fra 2008 med bl.a. pædagoger, terapeuter/behandlere, ledelse...

  4. Media och Multitasking : En studie om utbildningspodcast och träning

    OpenAIRE

    Björklund, Rickard; Weinz, Axel

    2017-01-01

    I och med ny medieteknik samt en allt mer uppkopplad och stressad värld har det blivit vanligare med multitasking. Tidigare forskning visar att 90 procent av befolkningen multitaskar någon gång varje dag. Detta trots att multitasking visats leda till både stress och försämrad prestationsförmåga i de aktiviteter man arbetar med, särskilt i samband med inlärning. Samtidigt har tidigare forskning visat att träning förbättrar flera funktioner i hjärnan kopplat till inlärning. I denna studie under...

  5. Tromboembolia pulmonar masiva, trombo en tránsito y disfunción ventricular derecha

    OpenAIRE

    Santos Martínez,Luis Efrén; Uriona Villarroel,Juan Eddy; Exaire Rodríguez,José Emilio; Mendoza,David; Martínez Guerra,María Luisa; Pulido,Tomás; Bautista,Edgar; Castañón,Alicia; Sandoval,Julio

    2007-01-01

    La tromboembolia pulmonar masiva se asocia con mortalidad alta. Es consecuencia de la migración de trombos del sistema venoso, aurícula o ventrículo derecho (trombo en tránsito) a la circulación pulmonar. Su repercusión hemodinámica dependerá del estado cardiopulmonar previo del sujeto y la magnitud de la obstrucción. La disfunción ventricular derecha aparecerá a mayor obstrucción y deterioro hemodinámico. El tratamiento de elección es la trombólisis periférica, asociada o no a la fragmentaci...

  6. Três Durações: Nelson, Glauber e Bressane

    Directory of Open Access Journals (Sweden)

    Alexandre Rocha da Silva

    2011-01-01

    Full Text Available Três Durações: Glauber, Bressane e Nelson identifica formas de brasilidade expressas pelo Cinema Novo. Recupera de Bergson os conceitos de  imagem-lembrança,  imagem-ação eduração para expressar indícios de brasilidade que ora se como identidade nacional ora como devir de um outro país imaginado pelo cinema. O Cinema Novo oferece formas de expressão remidiatizadas em que se visibilizam as estratégias micropolíticas que configuram a nacionalidade brasileira não mais somente como identidade, mas, sobretudo como memória.

  7. Core-free rolled actuators for Braille displays using P(VDF-TrFE-CFE)

    Science.gov (United States)

    Levard, Thomas; Diglio, Paul J.; Lu, Sheng-Guo; Rahn, Christopher D.; Zhang, Q. M.

    2012-01-01

    Refreshable Braille displays require many small diameter actuators to move the pins. The electrostrictive P(VDF-TrFE-CFE) terpolymer can provide the high strain and actuation force under modest electric fields that are required for this application. In this paper, we develop core-free tubular actuators and integrate them into a 3 × 2 Braille cell. The terpolymer films are solution cast, stretched to 6 μm thick, electroded, laminated into a bilayer, rolled into a 2 mm diameter tube, bonded, and provided with top and bottom contacts. Experimental testing of 17 actuators demonstrates significant strains (up to 4%) and blocking forces (1 N) at moderate electric fields (100 MV m-1). A novel Braille cell is designed and fabricated using six of these actuators.

  8. Discrete wavelet transform coupled with ANN for daily discharge forecasting into Três Marias reservoir

    Science.gov (United States)

    Santos, C. A. G.; Freire, P. K. M. M.; Silva, G. B. L.; Silva, R. M.

    2014-09-01

    This paper proposes the use of discrete wavelet transform (DWT) to remove the high-frequency components (details) of an original signal, because the noises generally present in time series (e.g. streamflow records) may influence the prediction quality. Cleaner signals could then be used as inputs to an artificial neural network (ANN) in order to improve the model performance of daily discharge forecasting. Wavelet analysis provides useful decompositions of original time series in high and low frequency components. The present application uses the Coiflet wavelets to decompose hydrological data, as there have been few reports in the literature. Finally, the proposed technique is tested using the inflow records to the Três Marias reservoir in São Francisco River basin, Brazil. This transformed signal is used as input for an ANN model to forecast inflows seven days ahead, and the error RMSE decreased by more than 50% (i.e. from 454.2828 to 200.0483).

  9. Síndrome de Sneddon relato de três casos

    OpenAIRE

    Zaccariotti,Vladimir Arruda; Martins,Luiz Fernando; Costa,Valter da; Silva,Nilzio A. da; Casas,Alberto A. Las; Melo-Souza,Sebastião Eurico de

    1995-01-01

    Descrita por Sneddon (196S) a síndrome consiste na ocorrência de acidente vascular encefálico (AVE) isquêmico em pacientes com livedo reticular. Trata-se de doença vascular sistêmica de causa desconhecida, em que há comprometimento de artérias de médio e pequeno calibres. Pode haver positividade dos anticorpos antifosfolípides. São apresentados três casos desta entidade, todos do sexo masculino com 7, 16 e 54 anos de idade. Em todos a instalação do quadro deu-se por convulsões focais seguidas...

  10. A TR-UWB Downconversion Autocorrelation Receiver for Wireless Body Area Network

    Directory of Open Access Journals (Sweden)

    Islam SMRiazul

    2009-01-01

    Full Text Available Low power UWB receiver architecture is proposed for a Wireless Body Area Network (WBAN. This receiving technology is a synergy of existing downconversion-based narrowband rejection mechanism in RF front end and signal processing in frequency domain. Frequency components of converted and filtered UWB pulses are separated into real and imaginary parts, independently correlated and effectively combined to achieve an improved output Signal to noise ratio (SNR. An extensive mathematical analysis has been performed to formulate the close-form expressions for SNRs in order to compare system performances toward favorable BER under BPSK modulation scheme. Analysis shows that optimal rotation of coordination plays an important role for the enhancement of receiving SNR which is further confirmed by computer simulation. A wide range of link level simulation (LLS urges that the proposed system is more power efficient in higher-order modulation (HOM schemes. Transmitted Reference (TR scheme has been considered as the basis for wideband communication.

  11. Violência em três quadros. Como falar sobre?

    OpenAIRE

    SOUZA, Carla Damêane P.

    2015-01-01

    Resumo: As relações entre comunicação e representações iconográficas nos dias atuais superam o esperado desde o descobrimento da fotografia e do cinema. Existem artistas que utilizam a arte para colaborar com a política da exploração de assuntos que estão na moda, outros tratam de questionar a maneira com que a arte é utilizada no que tange a disseminar pensamentos em relação a tais assuntos. Cotejando três objetos artísticos diferentes este texto pretende discorrer de que maneira a “violênci...

  12. T/R Multi-Chip MMIC Modules for 150 GHz

    Science.gov (United States)

    Samoska, Lorene A.; Pukala, David M.; Soria, Mary M.; Sadowy, Gregory A.

    2009-01-01

    Modules containing multiple monolithic microwave integrated-circuit (MMIC) chips have been built as prototypes of transmitting/receiving (T/R) modules for millimeter-wavelength radar systems, including phased-array radar systems to be used for diverse purposes that could include guidance and avoidance of hazards for landing spacecraft, imaging systems for detecting hidden weapons, and hazard-avoidance systems for automobiles. Whereas prior landing radar systems have operated at frequencies around 35 GHz, the integrated circuits in this module operate in a frequency band centered at about 150 GHz. The higher frequency (and, hence, shorter wavelength), is expected to make it possible to obtain finer spatial resolution while also using smaller antennas and thereby reducing the sizes and masses of the affected systems.

  13. Thermally Induced Chain Orientation for Improved Thermal Conductivity of P(VDF-TrFE) Thin Films

    Energy Technology Data Exchange (ETDEWEB)

    Green, Peter [National Renewable Energy Laboratory (NREL), Golden, CO (United States); Zhao, Junnan [University of Michigan; Tan, Aaron C. [University of Michigan

    2017-09-29

    The potential for polymer thin films to be used as coatings or dielectrics is limited by their low thermal conductivity, ..kappa... However, there have been very limited studies on ..kappa.. enhancement for polymer thin films. In this work, we show that the out-of-plane ..kappa.. of a poly(vinylidene fluoride-trifluoroethylene) (P(VDF-TrFE)) thin film can be enhanced when it undergoes the appropriate thermal annealing conditions. When the film is annealed above its melting temperature, we achieve a 300-400% increase in ..kappa.. across the measured temperature range, compared to the as-cast film. We attribute this enhancement to the extensive ordering of polymer backbone chains perpendicular to the substrate during the melt-recrystallization process.

  14. As três versões do neo-institucionalismo

    OpenAIRE

    Hall Peter A.; Taylor Rosemary C. R.

    2003-01-01

    O neo-institucionalismo não constitui uma corrente de pensamento unificada. Ao contrário, pelo menos três métodos de análise diferentes apareceram nessa área no último quarto de século: o institucionalismo histórico, o institucionalismo da escolha racional e o institucionalismo sociológico. Todas elas tratam, por ângulos diferentes, do papel desempenhado pelas instituições na determinação de resultados sociais e políticos. Expõe-se e examina-se a gênese de cada uma dessas variantes do "neo-in...

  15. Perspectivas de género en las condenas por tráfico de drogas

    Directory of Open Access Journals (Sweden)

    Luz M. Puente Aba

    2012-12-01

    Full Text Available This article pretends to examine sentences for drug trafficking from a gender perspective. An in-depth analysis of sentences and prison statistics from this point of view reveals that women are most affected by this repressive policy. The most remarkable fact is that while the number of convicted women in general is low, a large percentage of female offenders’ sentences are related to drug offences, thus representing a higher percentage in this group than in the male group. Yet if we analyze the prison population from a gender perspective, the results are even more striking: whereas women make up an insignificant percentage of the total, they nonetheless represent a large part of the total number of sentences for drug trafficking. Furthermore, a study of the characteristics of women sentenced for these crimes allows us to trace a specific profile for this group, which in turn provides an insight into why women are the main victims of the repressive drug policy, and the consequences they face. It will be analyzed whether penal treatment contributes to an excessive representation of the feminine gender in prisons; which gender sectors are particularly affected; and how we could find the causes and consequences of this situation. To conclude, new ways to solve this problem are suggested: reconsidering criminal policy on drugs, or strengthening alternatives to prison and different measures to support people serving sentences in prison. Este trabajo pretende analizar las condenas por tráfico de drogas desde una perspectiva de género. Partiendo de las estadísticas relativas al número y tipología de condenas por delitos, y asimismo de las estadísticas relativas a la población de los centros penitenciarios, se puede extraer que las mujeres son las más afectadas por la política penal en materia de tráfico de drogas, de carácter marcadamente represivo, puesto que suponen un porcentaje ínfimo de la población penitenciaria, y en cambio

  16. Research on Fault Rate Prediction Method of T/R Component

    Science.gov (United States)

    Hou, Xiaodong; Yang, Jiangping; Bi, Zengjun; Zhang, Yu

    2017-07-01

    T/R component is an important part of the large phased array radar antenna array, because of its large numbers, high fault rate, it has important significance for fault prediction. Aiming at the problems of traditional grey model GM(1,1) in practical operation, the discrete grey model is established based on the original model in this paper, and the optimization factor is introduced to optimize the background value, and the linear form of the prediction model is added, the improved discrete grey model of linear regression is proposed, finally, an example is simulated and compared with other models. The results show that the method proposed in this paper has higher accuracy and the solution is simple and the application scope is more extensive.

  17. A Crônica em Drummond: um gênero em trânsito

    OpenAIRE

    Pinho, Maria Helena da Silva Corrêa

    2011-01-01

    Esta dissertação de Mestrado analisa a obra de Carlos Drummond de Andrade, em particular a crônica - um gênero em trânsito. Para análise do corpus, escolhemos as crônicas: Divagação sobre as ilhas , Carta aos nascidos em maio , do livro Passeios na ilha(1962); Lembra-se de maio, Caso de escolha e Caso de ceguinho , do livro Cadeira de Balanço(1966) e Surge o poeta da flor do livro Caminhos de João Brandão(1970). Como objetivos específicos procuramos demonstrar a maestria com que ...

  18. Mulheres e tráfico de drogas: aprisionamento e criminologia feminista

    OpenAIRE

    Cortina,Monica Ovinski de Camargo

    2015-01-01

    O artigo examina o fenômeno das altas taxas do aprisionamento feminino no Brasil e sua relação ao crime de tráfico de drogas, sob a ótica da criminologia feminista e a da feminização da pobreza. O perfil das mulheres presas atende à seleção discriminatória do sistema penal, pois submete à prisão mulheres jovens, mães de mais de um filho ou de mais de uma filha, em vulnerabilidade social, com relatos de abuso de drogas e chefes de famílias monoparentais. Os resultados apontam para a necessidad...

  19. Isoflurane inhibits synaptic vesicle exocytosis through reduced Ca2+ influx, not Ca2+-exocytosis coupling.

    Science.gov (United States)

    Baumgart, Joel P; Zhou, Zhen-Yu; Hara, Masato; Cook, Daniel C; Hoppa, Michael B; Ryan, Timothy A; Hemmings, Hugh C

    2015-09-22

    Identifying presynaptic mechanisms of general anesthetics is critical to understanding their effects on synaptic transmission. We show that the volatile anesthetic isoflurane inhibits synaptic vesicle (SV) exocytosis at nerve terminals in dissociated rat hippocampal neurons through inhibition of presynaptic Ca(2+) influx without significantly altering the Ca(2+) sensitivity of SV exocytosis. A clinically relevant concentration of isoflurane (0.7 mM) inhibited changes in [Ca(2+)]i driven by single action potentials (APs) by 25 ± 3%, which in turn led to 62 ± 3% inhibition of single AP-triggered exocytosis at 4 mM extracellular Ca(2+) ([Ca(2+)]e). Lowering external Ca(2+) to match the isoflurane-induced reduction in Ca(2+) entry led to an equivalent reduction in exocytosis. These data thus indicate that anesthetic inhibition of neurotransmitter release from small SVs occurs primarily through reduced axon terminal Ca(2+) entry without significant direct effects on Ca(2+)-exocytosis coupling or on the SV fusion machinery. Isoflurane inhibition of exocytosis and Ca(2+) influx was greater in glutamatergic compared with GABAergic nerve terminals, consistent with selective inhibition of excitatory synaptic transmission. Such alteration in the balance of excitatory to inhibitory transmission could mediate reduced neuronal interactions and network-selective effects observed in the anesthetized central nervous system.

  20. A Model-Independent Algorithm to Derive Ca2+ Fluxes Underlying Local Cytosolic Ca2+ Transients

    Science.gov (United States)

    Ventura, Alejandra C.; Bruno, Luciana; Demuro, Angelo; Parker, Ian; Ponce Dawson, Silvina

    2005-01-01

    Local intracellular Ca2+ signals result from Ca2+ flux into the cytosol through individual channels or clusters of channels. To gain a mechanistic understanding of these events we need to know the magnitude and spatial distribution of the underlying Ca2+ flux. However, this is difficult to infer from fluorescence Ca2+ images because the distribution of Ca2+-bound dye is affected by poorly characterized processes including diffusion of Ca2+ ions, their binding to mobile and immobile buffers, and sequestration by Ca2+ pumps. Several methods have previously been proposed to derive Ca2+ flux from fluorescence images, but all require explicit knowledge or assumptions regarding these processes. We now present a novel algorithm that requires few assumptions and is largely model-independent. By testing the algorithm with both numerically generated image data and experimental images of sparklets resulting from Ca2+ flux through individual voltage-gated channels, we show that it satisfactorily reconstructs the magnitude and time course of the underlying Ca2+ currents. PMID:15681645

  1. Why live recording sounds better: A case study of Schumann’s Träumerei

    Directory of Open Access Journals (Sweden)

    Haruka eShoda

    2015-01-01

    Full Text Available We explore the concept that artists perform best in front of an audience. The negative effects of performance anxiety are much better known than their related cousin on the other shoulder: the positive effects of social facilitation. The present study, however, reveals a listener's preference for performances recorded in front of an audience. In Study 1, we prepared two types of recordings of Träumerei performed by 13 pianists: recordings in front of an audience and those with no audience. According to the evaluation by 153 listeners, the recordings performed in front of an audience sounded better, suggesting that the presence of an audience enhanced or facilitated the performance. In Study 2, we analyzed pianists' durational and dynamic expressions. According to the functional principal components analyses, we found that the expression of Träumerei consisted of three components: the overall quantity, the cross-sectional contrast between the final and the remaining sections, and the control of the expressive variability. Pianists' expressions were targeted more to the average of the cross-sectional variation in the audience-present than in the audience-absent recordings. In Study 3, we explored a model that explained listeners' responses induced by pianists' acoustical expressions, using path analyses. The final model indicated that the cross-sectional variation of the duration and that of the dynamics determined listeners' evaluations of the quality and the emotionally moving experience, respectively. In line with human's preferences for commonality, the more average the durational expressions were in live recording, the better the listeners' evaluations were regardless of their musical experiences. Only the well-experienced listeners (at least 16 years of musical training were moved more by the deviated dynamic expressions in live recording, suggesting a link between the experienced listener's emotional experience and the unique dynamics in

  2. TrAnsFuSE refines the search for protein function: oxidoreductases.

    Science.gov (United States)

    Harel, Arye; Falkowski, Paul; Bromberg, Yana

    2012-07-01

    Non-equilibrium catalysis of electron transfer reactions (i.e. redox) regulates the flux of key elements found in biological macromolecules. The enzymes responsible, oxidoreductases, contain specific transition metals in poorly sequence-conserved domains. These domains evolved ∼2.4 billion years ago in microbes and spread across the tree of life. We lack understanding of how oxidoreductases evolved; divergence of sequences makes identification difficult. We developed a method to recognise the various versions of these enzyme-domains in unannotated sequence-space. Often, homology is used to transfer function annotations from experimentally resolved domains to unannotated sequences. Unreliability of inferring homology below 30% sequence identity limits single-sequence based searches. Misaligned functional sites may compromise annotation transfer from even very similar sequences. Combining profile-based searches with knowledge of functional sites could improve domain detection accuracy. Here we present an approach that enhances the search for redox domains using catalytic site annotations. From the scientific literature, we validated annotations of 104 InterPro domains indicated as using "transition metals in redox reactions." These domains mediate electron transfer in 20% of oxidoreductases, primarily employing iron, copper and molybdenum. We used the experimentally identified catalytic residues in these domains to validate sequence alignment-based protein function annotations. Our method, TrAnsFuSE, is 11% and 14% more accurate than PSI-BLAST and InterPro, respectively. Moreover, it is robust for use with other functional residues-we attain higher accuracy at comparable coverage using metal binding, in addition to catalytic, sites. TrAnsFuSE can be used to focus the study of the vast amounts of unannotated sequencing data from meta-/genome projects.

  3. Actividades intersectoriales en la prevención de accidentes de tráfico

    Directory of Open Access Journals (Sweden)

    Peiró R.

    2003-01-01

    Full Text Available Se describen las líneas de trabajo priorizadas y algunos resultados obtenidos en la implantación del programa de prevención y reducción de lesiones por accidentes de tráfico en un área de salud. A partir de 1999 el Centro de Salud Pública priorizó la identificación y búsqueda de aliados en otros sectores (Asociación de policías locales y educación, la construcción de la capacidad de trabajo conjunta y la formación de un grupo intersectorial. Se llevaron a cabo actividades docentes y jornadas. Se formó una red de personas en 17 de un total de 39 ayuntamientos que participan con actividades en el grupo intersectorial. Un total de 10 de estos ayuntamientos participaron con actividades educativas y siete, además, exigieron al cumplimiento de la legislación. El grupo intersectorial potenció la visibilidad de sus actividades a través de los medios de comunicación local. Estas actividades no están dirigidas a reducir las lesiones por tráfico, sino a crear un marco de trabajo para la movilización de los sectores implicados. Se pretende potenciar más el compromiso entre los niveles políticos, técnicos y civiles incidiendo en valores sociales de respeto para una vida más saludable.

  4. Why live recording sounds better: a case study of Schumann's Träumerei.

    Science.gov (United States)

    Shoda, Haruka; Adachi, Mayumi

    2014-01-01

    We explore the concept that artists perform best in front of an audience. The negative effects of performance anxiety are much better known than their related cousin on the other shoulder: the positive effects of "social facilitation." The present study, however, reveals a listener's preference for performances recorded in front of an audience. In Study 1, we prepared two types of recordings of Träumerei performed by 13 pianists: recordings in front of an audience and those with no audience. According to the evaluation by 153 listeners, the recordings performed in front of an audience sounded better, suggesting that the presence of an audience enhanced or facilitated the performance. In Study 2, we analyzed pianists' durational and dynamic expressions. According to the functional principal components analyses, we found that the expression of "Träumerei" consisted of three components: the overall quantity, the cross-sectional contrast between the final and the remaining sections, and the control of the expressive variability. Pianists' expressions were targeted more to the "average" of the cross-sectional variation in the audience-present than in the audience-absent recordings. In Study 3, we explored a model that explained listeners' responses induced by pianists' acoustical expressions, using path analyses. The final model indicated that the cross-sectional variation of the duration and that of the dynamics determined listeners' evaluations of the quality and the emotionally moving experience, respectively. In line with human's preferences for commonality, the more "average" the durational expressions were in live recording, the better the listeners' evaluations were regardless of their musical experiences. Only the well-experienced listeners (at least 16 years of musical training) were moved more by the "deviated" dynamic expressions in live recording, suggesting a link between the experienced listener's emotional experience and the unique dynamics in music.

  5. The Historical X-ray Transient KY TrA in quiescence

    Science.gov (United States)

    Zurita, C.; Corral-Santana, J. M.; Casares, J.

    2015-12-01

    We present deep optical images of the historical X-ray Transient KY TrA in quiescence from which we confirm the identification of the counterpart reported by Murdin et al. and derive an improved position of α = 15:28:16.97 and δ = -61:52:57.8. In 2007 June we obtained I, R and V images, where the counterpart seems to be double indicating the presence of an interloper at ˜1.4 arcsec NW. After separating the contribution of KY TrA we calculate I = 21.47 ± 0.09, R = 22.3 ± 0.1 and V = 23.6 ± 0.1. Similar brightness in the I-band was measured in 2004 May and 2010 June. Variability was analysed from series of images taken in 2004, spanning 0.6 h, and in two blocks of 6 h during 2007. We find that the target is not variable in any data set above the error levels ˜0.07 mags. The presence of the interloper might explain the non-detection of the classic ellipsoidal modulation; our data indicates that it contributes around half of the total flux, which would make a variation <0.15 mag not detectable. A single spectrum obtained in 2004 May shows the H α emission characteristic of X-ray transients in quiescence with a full width at half-maximum FWHM = 2700 ± 280 km s-1. If the system follows the FWHM-K2 correlation found by Casares, this would correspond to a velocity semi-amplitude of the donor star of K2 = 630 ± 74 km s-1. Based on the outburst amplitude and colours of the optical counterpart in quiescence we derive a crude estimate of the orbital period of 8 h and an upper limit of 15 h which would lead to mass function estimates of ≈9 M⊙ and <16 M⊙, respectively.

  6. O mapa íntimo: três telas de Frida Kahlo Frida Kahlo's intimate map

    Directory of Open Access Journals (Sweden)

    Fabiano Seixas Fernandes

    2008-04-01

    Full Text Available O artigo apresenta análises de três telas da pintora mexicana Frida Kahlo - Las dos Fridas, Autorretrato en la frontera entre México y Estados Unidos e Lo que el agua me dio - e está dividido em três partes, cada qual tratando de uma tela em particular, para, no momento seguinte, ligar seu estudo ao das demais. Norteia a leitura das primeiras a discussão da relação nacional/estrangeiro no trabalho de Kahlo; a análise da terceira mostra a superação da dicotomia através do conceito de mapa íntimo, no qual entra em cena uma tênue e instável territorialização da memória através da representação pictórica.This article analyses three works by Mexican painter Frida Kahlo: Las dos Fridas (The Two Fridas, Autorretrato en la frontera entre México y Estados Unidos (Self-Portrait on the Borderline between Mexico and the United States, and Lo que el agua me dio (What the Water Gave Me. Each work is first analyzed separately and then connected with the others. The analysis of the first two is guided by the national/foreign dichotomy present in Kahlo; the analysis of the third shows the overcoming of the dichotomy through the concept of intimate map - in which a feeble and ever-changing territorialization of memory via plastic expression comes to show.

  7. Potencial discriminatório de três testadores em "topcrosses" de milho

    Directory of Open Access Journals (Sweden)

    Duarte Iran de Azevedo

    2003-01-01

    Full Text Available O objetivo deste trabalho foi determinar o potencial de três testadores endogâmicos elites a fim de avaliar e classificar 15 materiais genéticos para a síntese de híbridos. Quarenta e cinco híbridos mais quatro testemunhas foram avaliados em cinco ambientes, no ano agrícola 2000/2001, utilizando delineamento em blocos ao acaso, com três repetições. Foram avaliados os caracteres peso de grãos corrigido, altura de planta e altura de espiga. As estimativas de capacidade geral e específica de combinação foram obtidas pelo modelo de Griffing, adaptado por Vencovsky e Barriga, para análise em delineamento genético fatorial. O rendimento médio obtido entre os cinco ambientes variou de 8,97 t ha-1 a 12,21 t ha-1. Vinte e sete tratamentos superaram a média geral absoluta da melhor testemunha. A análise de variância conjunta revelou efeitos significativos de ambientes, capacidade geral de combinação dos parentais e testadores, capacidade específica de combinação e das interações ambiente x capacidade geral de combinação dos parentais e testadores. Os testadores promoveram uma classificação diferenciada para a base genética avaliada, com destaque para os cruzamentos entre parentais HS1, HS2, HS5 e HT4 com o testador LF e parentais L10 e HS5 com o testador L05.

  8. Role of Ca++ in Shoot Gravitropism. [avena

    Science.gov (United States)

    Rayle, D. L.

    1985-01-01

    A cornerstone in the argument that Ca(2+) levels may regulate growth is the finding the EGTA promotes straight growth. The usual explanation for these results is that Ca(2+) chelation from cell walls results in wall loosening and thus accelerated straight growth. The ability of frozen-thawed Avena coleoptile tissue (subjected to 15g tension) to extend in response to EGTA and Quin II was examined. The EGTA when applied in weakly buffered (i.e., 0.1mM) neutral solutions initiates rapid extension. When the buffer strength is increased, similar concentrations of EGTA produce no growth response. This implies when EGTA liberated protons are released upon Ca(2+) chelation they can either initiate acid growth (low buffer conditions) or if consumed (high buffer conditions) have no effect. Thus Ca(2+) chelation in itself apparently does not result in straight growth.

  9. Pygmy dipole resonance in stable Ca isotopes

    Energy Technology Data Exchange (ETDEWEB)

    Tertychny, G. [Institut fuer Kernphysik, Forschungszentrum Juelich, 52425 Juelich (Germany); Institute of Physics and Power Engineering, 249020 Obninsk (Russian Federation); Tselyaev, V. [Institut fuer Kernphysik, Forschungszentrum Juelich, 52425 Juelich (Germany); Institute of Physics S.Petersburg University (Russian Federation); Kamerdzhiev, S. [Institut fuer Kernphysik, Forschungszentrum Juelich, 52425 Juelich (Germany); Institute of Physics and Power Engineering, 249020 Obninsk (Russian Federation); Gruemmer, F. [Institut fuer Kernphysik, Forschungszentrum Juelich, 52425 Juelich (Germany); Krewald, S. [Institut fuer Kernphysik, Forschungszentrum Juelich, 52425 Juelich (Germany); Speth, J. [Institut fuer Kernphysik, Forschungszentrum Juelich, 52425 Juelich (Germany); Litvinova, E. [Institute of Physics and Power Engineering, 249020 Obninsk (Russian Federation); Avdeenkov, A. [Institute of Physics and Power Engineering, 249020 Obninsk (Russian Federation); Institut fuer Kernphysik, Forschungszentrum Juelich, 52425 Juelich (Germany)

    2007-05-15

    The properties of the low-lying electric dipole strength in the stable {sup 40}Ca,{sup 44}Ca and {sup 48}Ca isotopes have been calculated within the Extended Theory of Finite Fermi Systems (ETFFS). This approach is based on the random phase approximation (RPA) and includes the single-particle continuum as well as the coupling to low-lying collective states which are considered in a consistent microscopic way. For {sup 44}Ca we also include pairing correlations. A good agreement with the existing experimental data for the gross properties of the low-lying strength has been obtained. We conclude that for the detailed understanding of the low-lying dipole strength an approach like the present one is absolutely necessary.

  10. T-R CYCLE CHARACTERIZATION AND IMAGING: ADVANCED DIAGNOSTIC METHODOLOGY FOR PETROLEUM RESERVOIR AND TRAP DETECTION AND DELINEATION

    Energy Technology Data Exchange (ETDEWEB)

    Ernest A. Mancini

    2004-09-24

    The principal research effort for Year 1 of the project has been T-R cycle characterization and modeling. The research focus for the first nine (9) months of Year 1 was on outcrop study, well log analysis, seismic interpretation and data integration and for the remainder of the year the emphasis has been on T-R cycle model development. Information regarding the characteristics of T-R cycles has been assembled from the study of outcrops, from well log analyses, and from seismic reflection interpretation. From these studies, stratal boundaries separating T-R cycles have been found to be useful for the recognition and delineation of these cycles. The key stratal surfaces include subaerial unconformities, shoreface ravinement surfaces, transgressive surfaces, surfaces of maximum regression, and surfaces of maximum transgression. These surfaces can be identified and mapped in surface exposures and can be recognized in well log signatures and seismic reflection profiles as discontinuities. The findings from the study of outcrop, well log, and seismic reflection data are being integrated into a database for use in constructing a model for T-R cycle development.

  11. beta-TrCP inhibition reduces prostate cancer cell growth via upregulation of the aryl hydrocarbon receptor.

    Directory of Open Access Journals (Sweden)

    Udi Gluschnaider

    2010-02-01

    Full Text Available Prostate cancer is a common and heterogeneous disease, where androgen receptor (AR signaling plays a pivotal role in development and progression. The initial treatment for advanced prostate cancer is suppression of androgen signaling. Later on, essentially all patients develop an androgen independent stage which does not respond to anti hormonal treatment. Thus, alternative strategies targeting novel molecular mechanisms are required. beta-TrCP is an E3 ligase that targets various substrates essential for many aspects of tumorigenesis.Here we show that beta-TrCP depletion suppresses prostate cancer and identify a relevant growth control mechanism. shRNA targeted against beta-TrCP reduced prostate cancer cell growth and cooperated with androgen ablation in vitro and in vivo. We found that beta-TrCP inhibition leads to upregulation of the aryl hydrocarbon receptor (AhR mediating the therapeutic effect. This phenomenon could be ligand independent, as the AhR ligand 2,3,7,8-Tetrachlorodibenzo-p-Dioxin (TCDD did not alter prostate cancer cell growth. We detected high AhR expression and activation in basal cells and atrophic epithelial cells of human cancer bearing prostates. AhR expression and activation is also significantly higher in tumor cells compared to benign glandular epithelium.Together these observations suggest that AhR activation may be a cancer counteracting mechanism in the prostate. We maintain that combining beta-TrCP inhibition with androgen ablation could benefit advanced prostate cancer patients.

  12. Modelo ARIMA para pronosticar valores de tráfico en una red de datos Wi-Fi

    Directory of Open Access Journals (Sweden)

    Cesar Augusto Hernández Suarez

    2009-05-01

    Full Text Available El presente artículo de investigación científica y tecnológica tiene por objetivo demostrar que las series de tiempo son una exce- lente herramienta para el modelamiento de tráfico de datos en redes Wi-Fi. Para lograr este objetivo se utilizó la metodología de Box-Jenkins, la cual se describe. El modelamiento de tráfico Wi-Fi a través de modelos correlacionados como las series de tiem- po, permiten ajustar gran parte de la dinámica del comportamiento de los datos en una ecuación y con base en esto estimar va- lores futuros de tráfico. Lo anterior es una ventaja para la planeación de cobertura, reservación de recursos y la realización de un control más oportuno y eficiente en forma integrada a diferentes niveles de la jerarquía funcional de la red de datos Wi-Fi. Como resultado de la investigación se obtuvo un modelo de tráfico ARIMA de orden 6, el cual realizó pronósticos de tráfico con valores del error cuadrático medio relativamente pequeños, para un periodo de 18 días.

  13. Assessment of documentation of DSM-IV-TR Criteria A for diagnosis of schizophrenia in psychiatric unit, tertiary hospital, Malaysia.

    Science.gov (United States)

    Maung, K; Ohnmar, H; Than, W; Ramli, M; Najwa Hanim, M R; Ali Sabri, R; Ahmad Zafri, A B

    The purposes of this study were to investigate the documentation of the DSM-IV-TR- Criteria A in diagnoses of schizophrenia and to identify the symptoms associated with over diagnosis of schizophrenia. This study involved a retrospective review and analysis of data from case notes. Data of 107 newly diagnosed patients with schizophrenia were keyed in and analyzed using SPSS v 19. The cases were then evaluated for the use of the DSM-IV-TR- Criteria A. Over diagnosis was noted in 37.39% of the patients. Disorganised behaviour (12.5%), affective flattening (12.5%), hallucination (16%) and non-bizarre delusion (18.3%) significantly contributed to the over-diagnosis of schizophrenia. Symptoms such as non-bizarre delusion and hallucination were the most commonly used in over-diagnosing schizophrenia and were statistically significant with p ≤0.05. There was a significant lack of DSM-IV-TR Criteria A among the data documented to diagnose schizophrenia and non-bizarre delusion and hallucination were the most commonly used in over-diagnosing schizophrenia. This key problem needs to be addressed. The reliability of a diagnosis is indispensable and achievable with the proper clinical application of DSM-IV-TR Criteria A. The DSM-IV-TR Criteria have been perceived to be useful and reliable and is most widely used throughout the world.

  14. YouthCaN 2001 / Sirje Janikson

    Index Scriptorium Estoniae

    Janikson, Sirje

    2001-01-01

    Aprillis 2001 toimus keskkonnateemaline õpilaskonverents YouthCaN 2001 Ameerika Loodusajaloo Muuseumis New Yorkìs. 35 seminarist ühe viis läbi Tartu Noorte Loodusmaja geoloogia ja keskkonnaringi esindus, tutvustati loodusmaja keskkonnaprojekte ja räägiti keskkonnaalaste veebilehtede koostamise kogemustest. YouthCaN (Youth Communicating and Networking) on rahvusvaheline noorte organisatsioon, mis vahendab kogemusi ja uusi ideid elukeskkonnast huvitatud noorte hulgas

  15. Quantitative Analysis of Single-Nucleotide Polymorphism for Rapid Detection of TR34/L98H- and TR46/Y121F/T289A-Positive Aspergillus fumigatus Isolates Obtained from Patients in Iran from 2010 to 2014

    Science.gov (United States)

    Mohammadi, Faezeh; Hashemi, Seyed Jamal; Zoll, Jan; Melchers, Willem J. G.; Rafati, Haleh; Dehghan, Parvin; Rezaie, Sasan; Tolooe, Ali; Tamadon, Yalda; van der Lee, Henrich A.; Verweij, Paul E.

    2015-01-01

    We employed an endpoint genotyping method to update the prevalence rate of positivity for the TR34/L98H mutation (a 34-bp tandem repeat mutation in the promoter region of the cyp51A gene in combination with a substitution at codon L98) and the TR46/Y121F/T289A mutation (a 46-bp tandem repeat mutation in the promoter region of the cyp51A gene in combination with substitutions at codons Y121 and T289) among clinical Aspergillus fumigatus isolates obtained from different regions of Iran over a recent 5-year period (2010 to 2014). The antifungal activities of itraconazole, voriconazole, and posaconazole against 172 clinical A. fumigatus isolates were investigated using the European Committee on Antimicrobial Susceptibility Testing (EUCAST) broth microdilution method. For the isolates with an azole resistance phenotype, the cyp51A gene and its promoter were amplified and sequenced. In addition, using a LightCycler 480 real-time PCR system, a novel endpoint genotyping analysis method targeting single-nucleotide polymorphisms was evaluated to detect the L98H and Y121F mutations in the cyp51A gene of all isolates. Of the 172 A. fumigatus isolates tested, the MIC values of itraconazole (≥16 mg/liter) and voriconazole (>4 mg/liter) were high for 6 (3.5%). Quantitative analysis of single-nucleotide polymorphisms showed the TR34/L98H mutation in the cyp51A genes of six isolates. No isolates harboring the TR46/Y121F/T289A mutation were detected. DNA sequencing of the cyp51A gene confirmed the results of the novel endpoint genotyping method. By microsatellite typing, all of the azole-resistant isolates had genotypes different from those previously recovered from Iran and from the Dutch TR34/L98H controls. In conclusion, there was not a significant increase in the prevalence of azole-resistant A. fumigatus isolates harboring the TR34/L98H resistance mechanism among isolates recovered over a recent 5-year period (2010 to 2014) in Iran. A quantitative assay detecting a single

  16. Gold tetrahedra as building blocks in K3Au5Tr (Tr = In, Tl) and Rb2Au3Tl and in other compounds: a broad group of electron-poor intermetallic phases.

    Science.gov (United States)

    Li, Bin; Kim, Sung-Jin; Miller, Gordon J; Corbett, John D

    2009-07-20

    The alkali-metal gold trielides K(3)Au(5)Tr (Tr = In (I), Tl (II)) and Rb(2)Au(3)Tl (III) have been obtained directly from the elements, and their orthorhombic structures determined by single-crystal X-ray diffraction means (I/II: Imma, a = 5.562(1), 5.595(1); b = 19.645(4),19.706(4); c = 8.502(2), 8.430(2) A; Z = 4, respectively; III: Pmma, a = 5.660(1), b = 6.741(1), c = 9.045(2) A, Z = 4). These exhibit zigzag chains of Tr that link puckered sheets (I/II) or chains (III) of gold tetrahedra condensed through shared vertices. The segregation of Au and Tr components is striking relative to the evidently stronger and preferred Au-Tr bonding in neighboring gold- and alkali-metal-poorer triel phases. The close packing of K/Rb (A) about the gold tetrahedra gives each A and Au(4) component 7-10 and 10 neighbors of the other type, respectively. Tight-binding-linear-muffin-tin-orbital-atomic sphere approximation band structure calculations show that the title phases lie near or at electronic pseudogaps. The gold substructure is the dominant feature of the densities of states, with moderately broad 5d(10) features as favored by relativistic effects. Likewise, crystal orbital Hamilton population results indicate optimization of Au-Au bonding at the expense of the stronger heteroatomic Au-Tr interactions. Stabilization of these unusual structures appears to follow in part from the presence of numerous short and individually weak A-Au interactions, as manifested by appreciable mixing of s, p, and d valence orbitals on A into network bonding states, Au 5d in particular. These and related phases define a family of Au(4)-based phases with particularly low e/a values of 1.2 to approximately 2.3 (over all atoms, omitting Au 5d), closely related to the cubic Laves-type structures. The same region also contains Tr-richer tunnel and network structures with relatively fewer cations that also appear to be dominated by Au-Au and Au-Tr bonding.

  17. Study on the isospin equilibration phenomenon in nuclear reactions 40Ca + 40Ca , 40Ca + 46Ti , 40Ca + 48Ca , 48Ca + 48Ca at 25 MeV/nucleon by using the CHIMERA multidetector

    Science.gov (United States)

    Martorana, N. S.; Auditore, L.; Berceanu, I.; Cardella, G.; Chatterjee, M. B.; De Luca, S.; De Filippo, E.; Dell'Aquila, D.; Gnoffo, B.; Lanzalone, G.; Lombardo, I.; Maiolino, C.; Norella, S.; Pagano, A.; Pagano, E. V.; Papa, M.; Pirrone, S.; Politi, G.; Porto, F.; Quattrocchi, L.; Rizzo, F.; Russotto, P.; Trifirò, A.; Trimarchi, M.; Verde, G.; Vigilante, M.

    2017-11-01

    We report on the results obtained by studying nuclear reactions between isotopes of Ca and Ti at 25 MeV/nucleon. We used the multidetector CHIMERA to detect charged reaction products. In particular, we studied two main effects: the isospin diffusion and the isospin drift. In order to study these processes we performed a moving-source analysis on kinetic energy spectra of the isobar nuclei ^{3H} and ^{3He} . This method allows to isolate the emission from the typical sources produced in reactions at Fermi energy: projectile like fragment (PLF), target like fragment (TLF), and mid-velocity (MV) emission. The obtained results are compared to previous experimental investigations and to simulations obtained with CoMD-II model.

  18. Mitochondrial Ca2+ uptake in skeletal muscle health and disease

    CERN Document Server

    Zhou, Jingsong; Yi, Jianxun

    2016-01-01

    Muscle uses Ca2+ as a messenger to control contraction and relies on ATP to maintain the intracellular Ca2+ homeostasis. Mitochondria are the major sub-cellular organelle of ATP production. With a negative inner membrane potential, mitochondria take up Ca2+ from their surroundings, a process called mitochondrial Ca2+ uptake. Under physiological conditions, Ca2+ uptake into mitochondria promotes ATP production. Excessive uptake causes mitochondrial Ca2+ overload, which activates downstream adverse responses leading to cell dysfunction. Moreover, mitochondrial Ca2+ uptake could shape spatio-temporal patterns of intracellular Ca2+ signaling. Malfunction of mitochondrial Ca2+ uptake is implicated in muscle degeneration. Unlike non-excitable cells, mitochondria in muscle cells experience dramatic changes of intracellular Ca2+ levels. Besides the sudden elevation of Ca2+ level induced by action potentials, Ca2+ transients in muscle cells can be as short as a few milliseconds during a single twitch or as long as min...

  19. Izbor oblike vstopa na tuje tržišče z izdelki MLM Armal International GmbH

    OpenAIRE

    Brumen, Dragica

    2012-01-01

    Čas globalizacije, v katerem živimo, nas sili, da moramo biti zelo pazljivi in pozorno spremljati vsa dogajanja pri kupcih, kakor tudi dogajanja na njihovih tržiščih. Podjetje MLM bo še naprej v nenehni pripravljenosti in bo iskalo nova tržišča, saj se lahko hitro zgodi, da kateri kupec postane nelikviden oz. iz kateregakoli razloga ne posluje več in se tako izgubi določen del tržišča, ki ga je potrebno čim hitreje nadomestiti z novim. To je potrebno za doseganje ciljev, ki so načrtovani in z...

  20. A tríade “Verdadeiro-Bom-Belo”: O lugar da beleza na Idade Média

    Directory of Open Access Journals (Sweden)

    Jan A. Aertsen

    2008-06-01

    Full Text Available Neste artigo, Jan Aertsen discute a compreensão da tríade “Verdadeiro-Belo-Bom” como representante de um conjunto de questões clássicas em filosofia, a qual corresponderia, do ponto de vista moderno, à suposição de que o sujeito possui três modos distintos de se relacionar com o mundo: cognitivo, estético e prático. O autor sugere que a tríade foi formulada explicitamente pela primeira vez na Idade Média; neste período, entretanto, a beleza não era considerada um problema “estético”.

  1. [Forensic assessment of DSM-5 posttraumatic stress disorder: a commentary on the transition from DSM-IV-TR (I)].

    Science.gov (United States)

    Stevens, A; Fabra, M

    2013-12-01

    In May 2013 the American Psychiatric Association (APA) has released the latest and fifth edition of the diagnostic and statistical manual of mental disorders (DSM-5). Like its predecessor, the DSM-IV-TR, it will have considerable impact on the science of Psychiatry. The DSM-5 describes - actually available in English - the present medical knowledge about mental disorders. In the short run, German medical science and scientific medicolegal expertises will continue to rely on the German version of the DSM-IV-TR, however, they will be difficult to defend without bearing in mind the changes that DSM-5 brings about. This report discusses the transition from DSM-IV-TR to DSM-5 with regard to posttraumatic stress disorder (PTSD) and provides suggestions, how the criteria might be evaluated.

  2. Preparation and characterization of Eu 3 activated CaSiO3,(CaA ...

    Indian Academy of Sciences (India)

    Eu3+ activated CaSiO3, (Ca, Ba) SiO3 and (Ca, Sr) SiO3 have been prepared by sol–gel technique. Residual solvent and organic contents in the gel were removed by firing at 100°C for 3–4 h at 300 and 600°C for 2 h. Small exothermic shoulder around 850 to 875°C, as observed in DTA curve, corresponds to crystallization ...

  3. Ca(2+-dependent regulation of the Ca(2+ concentration in the myometrium mitochondria. II. Ca(2+ effects on mitochondria membranes polarization and [Ca(2+](m

    Directory of Open Access Journals (Sweden)

    L. G. Babich

    2017-06-01

    Full Text Available It is known that Ca2+ accumulation in the mitochondria undergoes complex regulation by Ca2+ itself. But the mechanisms of such regulation are still discussed. In this paper we have shown that Ca ions directly or indirectly regulate the level of myometrium mitochondria membranes polarization. The additions of 100 µM Ca2+ were accompanied by depolarization of the mitochondria membranes. The following experiments were designed to study the impact of Ca2+ on the myometrium mitochondria [Ca2+]m. Isolated myometrium mitochondria were preincubated without or with 10 μM Са2+ followed by 100 μM Са2+ addition. Experiments were conducted in three mediums: without ATP and Mg2+ (0-medium, in the presence of 3 mM Mg2+ (Mg-medium and 3 mM Mg2+ + 3 mM ATP (Mg,ATP-medium. It was shown that the effects of 10 μM Са2+ addition were different in different mediums, namely in 0- and Mg-medium the [Ca2+]m values increased, whereas in Mg,ATP-medium statistically reliable changes were not registered. Preincubation of mitochondria with 10 μM Са2+ did not affect the [Ca2+]m value after the addition of 100 μM Са2+. The [Ca2+]m values after 100 μM Са2+ addition were the same in 0- and Mg,ATP-mediums and somewhat lower in Mg-medium. Preliminary incubation of mitochondria with 10 μM Са2+ in 0- and Mg-mediums reduced changes of Fluo 4 normalized fluorescence values that were induced by 100 μM Са2+ additions, but in Mg,ATP-medium such differences were not recorded. It is concluded that Са2+ exchange in myometrium mitochondria is regulated by the concentration of Ca ions as in the external medium, so in the matrix of mitochondria. The medium composition had a significant impact on the [Са2+]m values in the absence of exogenous cation. It is suggested that light increase of [Са2+]m before the addition of 100 μM Са2+ may have a positive effect on the functional activity of the mitochondria.

  4. TransRapid TR-07 maglev-spectrum magnetic field effects on daily pineal indoleamine metabolic rhythms in rodents

    Energy Technology Data Exchange (ETDEWEB)

    Groh, K.R.

    1993-01-01

    This study examined the effects on pineal function of magnetic field (MF) exposures (ac and dc components) similar to those produced by the TransRapid TR-07 and other electromagnetic maglev systems (EMS). Rats were entrained to a light-dark cycle and then exposed to a continuous, or to an inverted, intermittent (on = 45 s, off = 15 s, induced current = 267 G/s) simulated multifrequency ac and dc magnetic field (MF) at 1 or 7 times the TR-07 maglev vehicle MF intensity for 2 hr. Other groups of rats were exposed to only the ac or the dc-component of the maglev MF. For comparison, one group was exposed to an inverted, intermittent 60-Hz MF. Each group was compared to an unexposed group of rats for changes in pineal melatonin and serotonin-N-acetyltransferase (NAT). MF exposures at an intensity equivalent to that produced by the TR-07 vehicle had no effect on melatonin or NAT compared with sham-exposed animals under any of the conditions examined. However, 7X TR-07-level continuous 2-h MF exposures significantly depressed pineal NAT by 45%. Pineal melatonin was also depressed 33--43% by a continuous 7X TR-07 MF exposure and 28% by an intermittent 60-Hz 850-mG MF, but the results were not statically significant. This study demonstrates that intermittent, combined ac and dc MFs similar to those produced by the TR-07 EMS maglev vehicle alter the normal circadian rhythm of pineal indoleamine metabolism. The pineal regulatory enzyme NAT was more sensitive to MF exposure than melatonin and may be a more desirable measure of the biological effects of MF exposure.

  5. TransRapid TR-07 maglev-spectrum magnetic field effects on daily pineal indoleamine metabolic rhythms in rodents

    Energy Technology Data Exchange (ETDEWEB)

    Groh, K.R.

    1993-06-01

    This study examined the effects on pineal function of magnetic field (MF) exposures (ac and dc components) similar to those produced by the TransRapid TR-07 and other electromagnetic maglev systems (EMS). Rats were entrained to a light-dark cycle and then exposed to a continuous, or to an inverted, intermittent (on = 45 s, off = 15 s, induced current = 267 G/s) simulated multifrequency ac and dc magnetic field (MF) at 1 or 7 times the TR-07 maglev vehicle MF intensity for 2 hr. Other groups of rats were exposed to only the ac or the dc-component of the maglev MF. For comparison, one group was exposed to an inverted, intermittent 60-Hz MF. Each group was compared to an unexposed group of rats for changes in pineal melatonin and serotonin-N-acetyltransferase (NAT). MF exposures at an intensity equivalent to that produced by the TR-07 vehicle had no effect on melatonin or NAT compared with sham-exposed animals under any of the conditions examined. However, 7X TR-07-level continuous 2-h MF exposures significantly depressed pineal NAT by 45%. Pineal melatonin was also depressed 33--43% by a continuous 7X TR-07 MF exposure and 28% by an intermittent 60-Hz 850-mG MF, but the results were not statically significant. This study demonstrates that intermittent, combined ac and dc MFs similar to those produced by the TR-07 EMS maglev vehicle alter the normal circadian rhythm of pineal indoleamine metabolism. The pineal regulatory enzyme NAT was more sensitive to MF exposure than melatonin and may be a more desirable measure of the biological effects of MF exposure.

  6. Data model and relational database design for the New Jersey Water-Transfer Data System (NJWaTr)

    Science.gov (United States)

    Tessler, Steven

    2003-01-01

    The New Jersey Water-Transfer Data System (NJWaTr) is a database design for the storage and retrieval of water-use data. NJWaTr can manage data encompassing many facets of water use, including (1) the tracking of various types of water-use activities (withdrawals, returns, transfers, distributions, consumptive-use, wastewater collection, and treatment); (2) the storage of descriptions, classifications and locations of places and organizations involved in water-use activities; (3) the storage of details about measured or estimated volumes of water associated with water-use activities; and (4) the storage of information about data sources and water resources associated with water use. In NJWaTr, each water transfer occurs unidirectionally between two site objects, and the sites and conveyances form a water network. The core entities in the NJWaTr model are site, conveyance, transfer/volume, location, and owner. Other important entities include water resource (used for withdrawals and returns), data source, permit, and alias. Multiple water-exchange estimates based on different methods or data sources can be stored for individual transfers. Storage of user-defined details is accommodated for several of the main entities. Many tables contain classification terms to facilitate the detailed description of data items and can be used for routine or custom data summarization. NJWaTr accommodates single-user and aggregate-user water-use data, can be used for large or small water-network projects, and is available as a stand-alone Microsoft? Access database. Data stored in the NJWaTr structure can be retrieved in user-defined combinations to serve visualization and analytical applications. Users can customize and extend the database, link it to other databases, or implement the design in other relational database applications.

  7. Hippocampal epileptiform activity induced by magnesium-free medium: differences between areas CA1 and CA2-3.

    Science.gov (United States)

    Lewis, D V; Jones, L S; Mott, D D

    1990-07-01

    Hippocampal slices, from which the entorhinal cortex had been removed, were exposed to artificial cerebrospinal fluid containing no magnesium (0-Mg ACSF) to elicit interictal bursts (IIBs) and electrographic seizures (EGSs). In 0-Mg ACSF, IIBs and EGSs occurred in both area CA1 and area CA3. The IIBs in CA3 led the IIBs in CA1 by several milliseconds. The epileptiform bursts occurring during the EGSs seemed to have the opposite relationship, with bursts in CA1 leading those in CA3 by several milliseconds. When the connections between CA1 and CA2-3 were cut, the IIBs ceased in CA1 and continued in CA3. To further characterize the local differences in epileptiform activity, totally separate minislices of area CA1 and area CA2-3 were prepared. In the CA2-3 minislices, a few EGSs occurred and thereafter only persistent IIBs prevailed. Conversely, in the CA1 minislices, many spontaneous EGSs occurred for long periods of time and no IIBs were seen. Periodic stimulation of the CA1 minislices triggered IIBs that suppressed the recurrent EGSs. In the hippocampal slice exposed to low magnesium, IIBs originate in CA2-3 and are propagated to CA1, where they can have a suppressant effect on EGSs. Furthermore, unlike IIBs, the bursts making up the EGSs seem to start in CA1 and invade CA2-3.

  8. Mg/Ca of Continental Ostracode Shells

    Science.gov (United States)

    Ito, E.; Forester, R. M.; Marco-Barba, J.; Mezquita, F.

    2007-12-01

    Marine ionic chemistry is thought to remain constant. This, together with the belief that marine calcifiers partition Mg/Ca in a systematic manner as functions of temperature (and Mg/Ca) of water forms the basis of the Mg/Ca thermometer. In continental settings both of these assumptions are usually not true. Continental waters contain a wide variety of solutes in absolute and relative ion concentrations. Hence, waters with identical Mg/Ca may have very different concentrations of Mg and Ca and very different anions. Here we use two examples to focus on the effects of ion chemistry on Mg/Ca partitioning in continental ostracode shells and we ignore the complexities of solute evolution, which can change Mg/Ca over timescales of minutes to millennia. Palacios-Fest and Dettman (2001) conducted a monthly study of ,Cypridopsis vidua at El Yeso Lake in Sonora, Mexico. They established a relation between temperature and average shell Mg/Ca using regression analyses on averaged data. When their Mg/Ca-temperature relation is applied to monthly ,C. vidua data from Page Pond near Cleveland, Ohio, water temperatures of -8 to -1°C are obtained. The observed Mg/Ca ranges for El Yeso Lake (0.31 to 0.46) and Page Pond (0.33 to 0.46) are similar, as are their specific conductivities (700 to 850μS for El Yeso Lake; 400 to 600μS for Page Pond). However, [Ca] is 140-260 mg/L for El Yeso, but only 70-90 mg/L for Page Pond. Page Pond data, in fact, shows a good temperature shell Mg/Ca relation for .C. vidua, but the relation is different from that at El Yeso. Hence, shell Mg/Ca is a multi-valued, family of curves function of temperature and Mg/Ca of water that depends on the [Mg] and [Ca] values in water and perhaps other factors. Our second example comes from sites near Valencia, Spain and involves shell data for ,Cyprideis torosa, an estuarine ostracode that is tolerant of a wide range of salinity and can live in continental waters as long as the carbonate alkalinity to Ca ratio is

  9. Radioisotope tracer studies of inorganic carbon and Ca in microbially derived CaCO3

    Science.gov (United States)

    Yates, Kimberly K.; Robbins, Lisa L.

    1999-01-01

    Microbial calcification significantly impacts the cycling and deposition of inorganic carbon. This research employs 45Ca and 14C techniques as radioisotopic tracers to examine the role of cellular cycling of Ca2+ and inorganic carbon in CaCO3 precipitation by the unicellular green alga Nannochloris atomus. Implications of the effects of these physiological aspects on CaCO3 precipitation and the effects of microbial calcification on CaCO3 δ13C ratios are discussed. Results from pulse/chase experiments indicate that intracellular Ca2+ is incorporated into extracellular CaCO3. Intracellular inorganic carbon leaks from cells within 10 to 12 s after injection of unlabelled NaHCO3, providing a source of inorganic carbon for extracellular CaCO3. Cellular expulsion of calcium plays a key role in increasing the CaCO3 saturation state at the site of calcification. The δ13C ratios of microbial carbonates may vary depending on the amount of photorespiratory CO2 incorporated.

  10. Negative feedback from CaSR signaling to aquaporin-2 sensitizes vasopressin to extracellular Ca2.

    Science.gov (United States)

    Ranieri, Marianna; Tamma, Grazia; Di Mise, Annarita; Russo, Annamaria; Centrone, Mariangela; Svelto, Maria; Calamita, Giuseppe; Valenti, Giovanna

    2015-07-01

    We previously described that high luminal Ca(2+) in the renal collecting duct attenuates short-term vasopressin-induced aquaporin-2 (AQP2) trafficking through activation of the Ca(2+)-sensing receptor (CaSR). Here, we evaluated AQP2 phosphorylation and permeability, in both renal HEK-293 cells and in the dissected inner medullary collecting duct, in response to specific activation of CaSR with NPS-R568. In CaSR-transfected cells, CaSR activation drastically reduced the basal levels of AQP2 phosphorylation at S256 (AQP2-pS256), thus having an opposite effect to vasopressin action. When forskolin stimulation was performed in the presence of NPS-R568, the increase in AQP2-pS256 and in the osmotic water permeability were prevented. In the freshly isolated inner mouse medullar collecting duct, stimulation with forskolin in the presence of NPS-R568 prevented the increase in AQP2-pS256 and osmotic water permeability. Our data demonstrate that the activation of CaSR in the collecting duct prevents the cAMP-dependent increase in AQP2-pS256 and water permeability, counteracting the short-term vasopressin response. By extension, our results suggest the attractive concept that CaSR expressed in distinct nephron segments exerts a negative feedback on hormones acting through cAMP, conferring high sensitivity of hormone to extracellular Ca(2+). © 2015. Published by The Company of Biologists Ltd.

  11. Effect of Ca content on equilibrium Ca isotope fractionation between orthopyroxene and clinopyroxene

    Science.gov (United States)

    Wang, Wenzhong; Zhou, Chen; Qin, Tian; Kang, Jin-Ting; Huang, Shichun; Wu, Zhongqing; Huang, Fang

    2017-12-01

    Concentration effect on equilibrium inter-mineral isotope fractionation is ubiquitous in solid solution systems, but it is not clear in which concentration range such effect is prominent. Using first-principles calculations, we examine the effect of Ca and Fe contents in orthopyroxene (opx) on its average Casbnd O bond length and the equilibrium Ca isotope fractionation factor (103lnα) between opx and clinopyroxene (cpx). Our results reveal that the average Casbnd O bond length in opx is much smaller than that in cpx and it does not change with variable Ca content x (x and y are mole ratios in CaxFeyMg1-x-ySiO3 thereafter here) when x ≤ 1/48. Incorporation of Fe (y ≥ 1/32) into opx with a fixed Ca content can only slightly increase the average Casbnd O bond length. 103lnαopx-cpx of 44Ca/40Ca is linearly correlated with the average Casbnd O bond length in opx, suggesting that 103lnαopx-cpx of 44Ca/40Ca is controlled by opx Casbnd O bond strength. Our calculations indicate that the Ca concentration effect on 103lnαopx-cpx is significant when x in opx ranges from 2/16 to 1/48, while Fe in natural opx only causes a slight decrease in 103lnαopx-cpx. Our results provide insights into Ca isotope fractionation in high-temperature geochemical processes. Given that Ca content x in opx from natural peridotites is usually lower than 1/32 and Fe content y is generally ∼10 mol%, Ca and Fe concentration effects on 103lnαopx-cpx in natural samples are negligible. Rather, 103lnαopx-cpx is mainly controlled by temperature. 103lnαopx-cpx of 44Ca/40Ca decreases from 0.50‰ to 0.26‰ when temperature increases from 1000 K to 1400 K if the Fe effect is taken into account. Therefore, if Ca isotope fractionation between opx and cpx (Δ44/40Caopx-cpx) in natural peridotites is greater than 0.50‰ or lower than 0.26‰, it may indicate disequilibrium of Ca isotopes. Finally, the large 103lnαopx-cpx relative to our current analytical precision suggests that Δ44/40Caopx

  12. Physical conditions in CaFe interstellar clouds

    OpenAIRE

    Gnacinski, P.; Krogulec, M.

    2007-01-01

    Interstellar clouds that exhibit strong Ca I and Fe I lines were called CaFe clouds. The ionisation equilibrium equations were used to model the column densities of Ca II, Ca I, K I, Na I, Fe I and Ti II in CaFe clouds. The chemical composition of CaFe clouds is that of the Solar System and no depletion of elements onto dust grains is seen. The CaFe clouds have high electron densities n=1 cm^-3 that leads to high column densities of neutral Ca and Fe.

  13. Absolute Ca Isotopic Measurement Using an Improved Double Spike Technique

    Directory of Open Access Journals (Sweden)

    Jason Jiun-San Shen

    2009-01-01

    Full Text Available A new vector analytical method has been developed in order to obtain the true isotopic composition of the 42Ca-48Ca double spike. This is achieved by using two different sample-spike mixtures combined with the double spike and natural Ca data. Be cause the natural sample (two mixtures and the spike should all lie on a single mixing line, we are able to con strain the true isotopic composition of our double spike using this new approach. Once the isotopic composition of the Ca double spike is established, we are able to obtain the true Ca isotopic composition of the NIST Ca standard SRM915a, 40Ca/44Ca = 46.537 ± 2 (2sm, n = 55, 42Ca/44Ca = 0.31031 ± 1, 43Ca/44Ca = 0.06474 ± 1, and 48Ca/44Ca = 0.08956 ± 1. De spite an off set of 1.3% in 40Ca/44Ca between our result and the previously re ported value (Russell et al. 1978, our data indicate an off set of 1.89__in 40Ca/44Ca between SRM915a and seawater, entirely consistent with the published results.

  14. Elektrophysiologische Korrelate von beeinträchtigter musikalischer Klang-Wahrnehmung bei Cochlea-Implantat-Trägern

    OpenAIRE

    Sandmann, P; Kegel, A; Eichele, T; Dillier, N; Lai, W K; Bendixen, A; Debener, S; Jäncke, Lutz; Meyer, Martin

    2010-01-01

    Viele Träger eines Cochlea-Implantats (CI) können Sprache gut diskriminieren, während die Musik-Wahrnehmung mit einem CI oftmals beklagt wird. Schlechte Musik-Wahrnehmung bei CI-Trägern kann durch den defizitären Input des CIs erklärt werden, weil Informationen zur Frequenz und zeitlichen Feinstruktur nur begrenzt durch das CI übermittelt werden (Drennan und Rubinstein, 2008). Die Wirksamkeit des CIs hängt jedoch nicht nur von der Qualität des CI-Signals ab, sondern auch von der Anpassung des...

  15. Influencia del flujo de tráfico vehicular en la contaminación sonora del Cercado de Lima

    OpenAIRE

    Visaga Fernández, Sandra Indira; E. P. de Ingeniería Ambiental, Universidad Peruana Unión - Lima

    2016-01-01

    En este trabajo, se ha realizado una medición experimental de los niveles de ruido en 61 puntos estratégicos previamente definidos mediante una evaluación de la distribución espacial del Cercado de Lima, y a la vez en estosmismos puntos se ha recolectado información sobre el flujo del tráfico vehicular para determinar su influencia en la contaminación sonora. Para la recolección de data de campo; niveles de ruido y flujo de tráfico vehicular, se hadeterminado una distribución temporal, compre...

  16. Relación productividad - diversidad dentro y entre niveles tróficos de un ecosistema de pastizal

    OpenAIRE

    Piñeiro Guerra, Juan Manuel

    2014-01-01

    Existe un interés histórico por las relaciones diversidad-productividad dentro y entre niveles tróficos y las relaciones diversidad-diversidad y productividad-productividad entre niveles tróficos. Sin embargo, no existe consenso en la forma de las relaciones, sus determinantes, los mecanismos subyacentes o la dirección causal entre las variables. El objetivo central fue analizar las relaciones mencionadas en un ecosistema de pastizal inundable a diferentes escalas espacio-temporales a lo larg...

  17. T-R Cycle Characterization and Imaging: Advanced Diagnostic Methodology for Petroleum Reservoir and Trap Detection and Delineation

    Energy Technology Data Exchange (ETDEWEB)

    Ernest A. Mancini

    2006-08-30

    Characterization of stratigraphic sequences (T-R cycles or sequences) included outcrop studies, well log analysis and seismic reflection interpretation. These studies were performed by researchers at the University of Alabama, Wichita State University and McGill University. The outcrop, well log and seismic characterization studies were used to develop a depositional sequence model, a T-R cycle (sequence) model, and a sequence stratigraphy predictive model. The sequence stratigraphy predictive model developed in this study is based primarily on the modified T-R cycle (sequence) model. The T-R cycle (sequence) model using transgressive and regressive systems tracts and aggrading, backstepping, and infilling intervals or sections was found to be the most appropriate sequence stratigraphy model for the strata in the onshore interior salt basins of the Gulf of Mexico to improve petroleum stratigraphic trap and specific reservoir facies imaging, detection and delineation. The known petroleum reservoirs of the Mississippi Interior and North Louisiana Salt Basins were classified using T-R cycle (sequence) terminology. The transgressive backstepping reservoirs have been the most productive of oil, and the transgressive backstepping and regressive infilling reservoirs have been the most productive of gas. Exploration strategies were formulated using the sequence stratigraphy predictive model and the classification of the known petroleum reservoirs utilizing T-R cycle (sequence) terminology. The well log signatures and seismic reflector patterns were determined to be distinctive for the aggrading, backstepping and infilling sections of the T-R cycle (sequence) and as such, well log and seismic data are useful for recognizing and defining potential reservoir facies. The use of the sequence stratigraphy predictive model, in combination with the knowledge of how the distinctive characteristics of the T-R system tracts and their subdivisions are expressed in well log patterns

  18. Estudio científico de la accidentalidad de tránsito en el cantón Cuenca

    OpenAIRE

    Salinas Cabrera, Marcos Esteban; Vele Figueroa, Luis Henry

    2014-01-01

    El estudio muestra un análisis del número de accidentes y muertes de tránsito ocurridos en el cantón Cuenca durante los últimos siete años, con el fin de evaluar el impacto que han tenido las reformas de leyes y campañas en materia de tránsito, y posteriormente, empleando series temporales predecir el número de accidentes y fallecidos hasta el año 2016. The study shows an analysis of the number of accidents and traffic deaths that occurred in Cuenca during the past seven years, in order to...

  19. Toma de decisiones rentables mediante la contabilidad del trúput en una lavandería industrial

    OpenAIRE

    Pérez-Henao, C. (Catalina); Arcos-Henao, L. F. (Luis Fernando)

    2009-01-01

    Se presenta un nuevo enfoque para la toma de decisiones en una lavandería industrial mediante la aplicación de la contabilidad del trúput, concepto de la teoría de restricciones (TOC). Para identificar la restricción en el sistema se realiza el análisis de los procesos de lavado especial a partir de su capacidad. Se efectuó en cada proceso el análisis del octano (velocidad del trúput) para identificar la línea que genera mayor velocidad en la facturación y, por ende, a la cual se ...

  20. Extracorporeal Shock Wave Rebuilt Subchondral Bone In Vivo and Activated Wnt5a/Ca2+ Signaling In Vitro

    Directory of Open Access Journals (Sweden)

    Lai Yu

    2017-01-01

    Full Text Available Background. This study aimed to identify the optimal extracorporeal shock wave (ESW intensity and to investigate its effect on subchondral bone rebuilt in vivo and Wnt5a/Ca2+ signaling in vitro using an osteoarthritis (OA rat model and bone marrow mesenchymal stem cells (BMMSCs, respectively. Methods. OA rats treated with (OA + ESW group or without (OA group ESW (n=12/group were compared with healthy controls (control group, n=12. Gait patterns and subchondral trabecular bone changes were measured. Western blot and quantitative real-time polymerase chain reaction detected protein expression and gene transcription, respectively. Results. The gait disturbances of OA + ESW group were significantly improved compared with the OA group at 6th and 8th weeks. The micro-CT analysis indicated that the BMD, BSV/BV, BV/TV, Tr.S, and Tr.Th are significantly different between OA group and OA + ESW group. Expression of Wnt5a was increased rapidly after ESW treatment at 0.6 bar and peaked after 30 min. Conclusions. ESW were positive for bone remodeling in joint tibial condyle subchondral bone of OA rat. ESW prevented histological changes in OA and prevented gait disturbance associated with OA progression. Optimal intensity of ESW induced changes in BMMSCs via activation of the Wnt5a/Ca2+ signaling pathway.

  1. Comorbilidades psiquiátricas en los trastornos del espectro autista: estudio comparativo entre los criterios DSM-IV-TR y DSM-5

    Directory of Open Access Journals (Sweden)

    Marina Romero

    2016-01-01

    Full Text Available Antecedentes/Objetivo: Los Trastornos del Espectro Autista (TEA incluyen un grupo heterogéneo en cuanto a su presentación clínica, lo que supone un desafío a nivel de caracterización diagnóstica. Por consiguiente, el objetivo principal de la clasificación DSM-5 debería de ser identificar subgrupos de ASD que incluyan severidad y comorbilidades psiquiátricas. El objetivo principal de este estudio es explorar las comorbilidades diagnósticas que pueden ser relevantes como descriptores de fenotipos autistas así como la severidad de los síntomas de autismo y comparar los resultados de las diferentes criterios de clasificación entre el DSM-IV-TR y el DSM-5. Método: Se realiza un estudio comparati - vo de severidad y comorbilidades psiquiátricas entre una muestra con diagnóstico de Trastorno Generalizado del Desarrollo, según criterios DSM-IV-TR, y una muestra que cumplía también criterios para TEA según la clasificación DSM-5. La muestra fue obtenida en centros educativos ( n =123. Las comorbilidades psiquiátricas y la severidad de los síntomas se evaluaron a través del The Nisonger Child Behavior Rating Form , entrevista clínica y el Inventario de Trastorno del Espectro Autista, respectivamente. Las comorbi - lidades estudiadas fueron ansiedad, alteraciones de la conducta alimentaria, auto-agre - sividad, hetero-agresividad, autolesiones, trastorno obsesivo-compulsivo y déficit de atención e hiperactividad. Resultados: Se encontraron diferencias estadísticamente sig - nificativas entre ambos grupos para trastorno obsesivo-compulsivo , alteraciones de la conducta alimentaria y severidad . Conclusiones: Se apoya la hipótesis de que los indivi - duos que cumplen criterios diagnósticos según DSM-5 tienen mayor severidad sintomáti - ca, no sólo con respecto a los síntomas autistas centrales, sino también en relación con comorbilidades psiquiátricas.

  2. Aportació al coneixement de l’herbari Trèmols de l’Institut Botànic de Barcelona

    Directory of Open Access Journals (Sweden)

    Gavioli, L.

    2013-12-01

    ha llevado a cabo un inventario a nivel de género de todos los volúmenes y se ha informatizado el catálogo original del herbario y el fichero taxonómico existente. También se ha elaborado un inventario preliminar del contenido de las cajas adicionales. El análisis de los 3695 especímenes revela que el herbario está formado mayoritariamente (67,1% por pliegos de intercambio obtenidos principalmente a través de la Société Helvétique pour l’Échange des Plantes y la Societat Botànica Barcelonesa. Entre los materiales estudiados se han localizado dos pliegos que consideramos interesantes desde un punto de vista taxonómico y/o nomenclatural (isótipos de Silene holzmanii Heldr. ex Boiss. y de Arenaria minutiflora Loscos. El interés principal del herbario radica, pues, en la gran diversidad de procedencias geográficas de las plantas, que hacen de esta colección uno de los primeros «herbarios europeos» de Cataluña. [ca] L’herbari BC-Trèmols fou creat durant la segona meitat del segle XIX pel químic i botànic català Frederic Trèmols i Borrell. Entre 1930 i 1960, el tècnic Antoni Marcos va fer-ne una profunda revisió. La col·lecció actual consta aproximadament d’uns 9000 plecs; està formada per 58 volums reordenats per Marcos, dos volums amb l’estructura original, quatre capses de Hieracium i 85 capses de material addicional. Fins ara s’han informatitzat 22 dels volums i les quatre capses de Hieracium (3695 espècimens, s’ha realitzat un inventari a nivell de gènere de tots els volums i s’ha informatitzat el catàleg original de l’herbari i el fitxer taxonòmic existent. També s’ha elaborat un inventari preliminar del contingut de les capses addicionals. L’anàlisi dels 3695 espècimens fa evident que l’herbari està format majoritàriament (67,1% per plecs d’intercanvi obtinguts sobretot mitjançant la Société Helvétique pour l’Échange des i la Societat Botànica Barcelonesa. Entre els materials estudiats s

  3. Digitally Calibrated TR Modules Enabling Real-Time Beamforming SweepSAR Architectures

    Science.gov (United States)

    Hoffman, James P.; Veilleux, Louse A.; Peral, Eva; Chuang, Chung-Lun; Shaffer, Scott J.

    2013-01-01

    SweepSAR, a novel radar architecture that depends on a DBF (digital beamforming) array, requires calibration accuracies that are order(s) of magnitude greater than is possible with traditional techniques, such as a priori characterization of TR (transmit/receive) modules in thermal vacuum chambers, or simple loop-back of the calibration signal. The advantages of a SweepSAR architecture are so great that it is worth applying significant resources to calibration efforts. Due to the nature of the DBF, each channel contains a digitizer and very powerful digital processor. Each channel can independently digitize (with the digitizer) and analyze (with the processor) its channel's unique calibration signal, and extract the relevant calibration parameters, namely channel gain and channel phase delay commonly referred to as the gain (or amplitude) and phase of the channel. Using the processor, each channel's gain and phase can theoretically be estimated with arbitrary precision through averaging a sufficiently large number of samples. Systematic errors and the changing gain and phase of the channels, typically due to temperature drifts, limits how long the averaging can occur, which limits the precision of the calibration estimate. However, results indicate that calibration knowledge of both the transmit and receive chains of each TR module can be improved by one or two orders of magnitude. Due to the digital nature of the receiver data, the channel's gain and phase may be corrected by a similar amount, while the transmit chain can only be corrected in a traditional manner. To implement Sweep SAR, the order of magnitude improvement in the knowledge of the channel's gain and phase is needed, and the control of the receiver to a similar level is required. Inherent to the DBF array is the individual digitization of each of the array's receiver channels. Current systems typically combine all of the analog signals in the array into one or two analog channels, which are then

  4. Compressibilidade de três Latossolos em função da umidade e uso

    Directory of Open Access Journals (Sweden)

    M. K. Kondo

    1999-06-01

    Full Text Available Por meio do ensaio de compressibilidade, foram estudados os efeitos do manejo e da umidade na pressão de preconsolidação (σp de três solos: Latossolo Vermelho-Amarelo, Latossolo Roxo e Latossolo Vermelho-Escuro sob cultura anual, mata natural e pastagem, na região de Lavras (MG, em duas profundidades (0-0,03 e 0,27-0,30 m. Para cada condição, foram coletadas cinco amostras indeformadas e uma deformada, com três repetições. As amostras, indeformadas e com diferentes umidades, foram utilizadas no ensaio de compressão uniaxial, obtendo-se as curvas de compressão, das quais foram extraídas as respectivas pressões de preconsolidação. Com as amostras deformadas, determinaram-se os limites de plasticidade e de contração, textura e matéria orgânica. Os modelos de compactação testados foram baseados na pressão de preconsolidação e na umidade do solo. Para uma mesma condição e profundidade, houve diferença significativa entre os valores dos teores de argila e areia nos três solos. Os valores da densidade do solo inicial (Ds i foram estatisticamente diferentes para todas as condições na mesma profundidade, exceto na camada de 0-0,03 m para a cultura anual. À medida que a umidade do solo aumentou, a pressão de preconsolidação decresceu exponencialmente, indicando uma redução na capacidade de suporte de carga do solo. O LR apresentou, em geral, maior capacidade de suporte de carga do que o LE e LV. Essa maior capacidade de suporte de carga pode estar associada com o seu maior teor de argila e menor teor de areia. A capacidade de suporte de carga na zona de friabilidade variou de 154 a 167 kPa, para o LV; de 77 a 183 kPa, para o LR, e de 77 a 132 kPa, para o LE. As umidades 0,33 a 0,30 kg kg-1, 0,42 a 0,27 kg kg-1, e 0,35 a 0,33 kg kg-1 correspondem à faixa de friabilidade (LP - LC do LV, LR e LE, respectivamente. O modelo baseado na história de tensão do solo evidenciou o efeito da compactação causada pelas m

  5. Sør-Trøndelag Aktivitets-Relaterte Tiltak (START – Samhandling for bedre helse

    Directory of Open Access Journals (Sweden)

    Tommy Aune Rehn

    2011-08-01

    Full Text Available Høy forekomst av kroniske sykdommer og stigende helseutgifter representerer i lys av den kommende eldrebølgenen stor samfunnsøkonomisk utfordring. Den negative helseutviklingen er i ferd med å bli et nasjonalt problem og må få konsekvenser for fremtidig norsk helsepolitikk. Fysisk inaktivitet er en livsstilsfaktor som er sterkt assosiert med lavt nivå av kondisjon, total dødelighet og død av hjerte- og karsykdom. Studier viser at selv en liten bedring i kondisjon forventes å gi betydelig økt forventet levealder og at individer som har et bra kondisjonsnivå har opptil 15 flere leveår med god helse hvor de er selvhjulpne. Økt fysisk aktivitet har en gunstig effekt på alle risikofaktorer for kronisk sykdom og tidlig død, og vi mener derfor at man bør legge til rette for å øke det generelle fysiske aktivitetsnivået i befolkningen og bedre kunnskapen om fysisk aktivitet. Økt fysisk aktivitet vil forventes å bedre folkehelsen ved å redusere sykdomsforekomst, medisinbruk og dødelighet samt øke arbeidsevnen i populasjonen, noe som igjen vil redusere de samfunnsøkonomiske utgiftene. For å lykkes med dette, har vi tatt initiativ til prosjektet ”Sør-Trøndelag Aktivitets-Relaterte Tiltak” (START, som er tenkt å være en koordinert populasjonsbasert samhandling for bedre helse gjennom økt fysisk aktivitet rettet mot alle lag i befolkningen, fra krybbe til grav. Målet er at START skal danne grunnlag for nasjonal implementering av tiltak slik at vi oppnår en helsepolitisk bærekraftig utvikling i årene som kommer. Denne artikkelenbeskriver START og bakgrunnen for prosjektet.Rehn TA, Rognmo Ø, Ingebrigtsen JE, Vik JT, Wisløff U. Sør-Trøndelag Activity-Related Trial (START –Cooperation for better health. Nor J Epidemiol 2011; 20 (2: 209-216.ENGLISH SUMMARYHigh incidence of chronic diseases and rising health expenditure represents in light of the increasing proportion of elderly in our society a development that cannot

  6. Swelling-activated Ca2+ channels trigger Ca2+ signals in Merkel cells.

    Directory of Open Access Journals (Sweden)

    Henry Haeberle

    2008-03-01

    Full Text Available Merkel cell-neurite complexes are highly sensitive touch receptors comprising epidermal Merkel cells and sensory afferents. Based on morphological and molecular studies, Merkel cells are proposed to be mechanosensory cells that signal afferents via neurotransmission; however, functional studies testing this hypothesis in intact skin have produced conflicting results. To test this model in a simplified system, we asked whether purified Merkel cells are directly activated by mechanical stimulation. Cell shape was manipulated with anisotonic solution changes and responses were monitored by Ca2+ imaging with fura-2. We found that hypotonic-induced cell swelling, but not hypertonic solutions, triggered cytoplasmic Ca2+ transients. Several lines of evidence indicate that these signals arise from swelling-activated Ca2+-permeable ion channels. First, transients were reversibly abolished by chelating extracellular Ca2+, demonstrating a requirement for Ca2+ influx across the plasma membrane. Second, Ca2+ transients were initially observed near the plasma membrane in cytoplasmic processes. Third, voltage-activated Ca2+ channel (VACC antagonists reduced transients by half, suggesting that swelling-activated channels depolarize plasma membranes to activate VACCs. Finally, emptying internal Ca2+ stores attenuated transients by 80%, suggesting Ca2+ release from stores augments swelling-activated Ca2+ signals. To identify candidate mechanotransduction channels, we used RT-PCR to amplify ion-channel transcripts whose pharmacological profiles matched those of hypotonic-evoked Ca2+ signals in Merkel cells. We found 11 amplicons, including PKD1, PKD2, and TRPC1, channels previously implicated in mechanotransduction in other cells. Collectively, these results directly demonstrate that Merkel cells are activated by hypotonic-evoked swelling, identify cellular signaling mechanisms that mediate these responses, and support the hypothesis that Merkel cells contribute

  7. Analysing force-pCa curves.

    Science.gov (United States)

    Walker, John S; Li, Xiaotao; Buttrick, Peter M

    2010-07-01

    We investigated three forms of the Hill equation used to fit force-calcium data from skinned muscle experiments; Two hyperbolic forms that relate force to calcium concentration directly, and a sigmoid form that relates force to the -log(10) of the calcium concentration (pCa). The equations were fit to force-calcium data from 39 cardiac myocytes (up to five myocytes from each of nine mice) and the Hill coefficient and the calcium required for half maximal activation, expressed as a concentration (EC(50)) and as a pCa value (pCa(50)) were obtained. The pCa(50) values were normally distributed and the EC(50) values were found to approximate a log-normal distribution. Monte Carlo simulations confirmed that these distributions were intrinsic to the Hill equation. Statistical tests such as the t-test are robust to moderate levels of departure from normality as seen here, and either EC(50) or pCa(50) may be used to test for significant differences so long as it is kept in mind that ΔEC₅₀ is an additive measure of change and that ΔpCa₅₀ is a ratiometric measure of change. The Hill coefficient was found to be sufficiently log-normally distributed that log-transformed values should be used to test for statistically significant differences.

  8. Diffusion of Ca and Mg in Calcite

    Energy Technology Data Exchange (ETDEWEB)

    Cygan, R.T.; Fisler, D.K.

    1999-02-10

    The self-diffusion of Ca and the tracer diffusion of Mg in calcite have been experimentally measured using isotopic tracers of {sup 25}Mg and {sup 44}Ca. Natural single crystals of calcite were coated with a thermally-sputtered oxide thin film and then annealed in a CO{sub 2} gas at one atmosphere total pressure and temperatures from 550 to 800 C. Diffusion coefficient values were derived from the depth profiles obtained by ion microprobe analysis. The resultant activation energies for Mg tracer diffusion and Ca self-diffusion are respectively: E{sub a}(Mg) = 284 {+-} 74 kJ/mol and E{sub a}(Ca) = 271 {+-} 80 kJ/mol. For the temperature ranges in these experiments, the diffusion of Mg is faster than Ca. The results are generally consistent in magnitude with divalent cation diffusion rates obtained in previous studies and provide a means of interpreting the thermal histories of carbonate minerals, the mechanism of dolomitization, and other diffusion-controlled processes. The results indicate that cation diffusion in calcite is relatively slow and cations are the rate-limiting diffusing species for the deformation of calcite and carbonate rocks. Application of the calcite-dolomite geothermometer to metamorphic assemblages will be constrained by cation diffusion and cooling rates. The direct measurement of Mg tracer diffusion in calcite indicates that dolomitization is unlikely to be accomplished by Mg diffusion in the solid state but by a recrystallization process.

  9. Treatment of Ruptured Ovarian Endometrioma with Extremely High CA 125, Moderately High CA 19-9 and CA 15-3 Level

    Directory of Open Access Journals (Sweden)

    Muzeyyen Duran

    2013-06-01

    Full Text Available In this case report, a ruptured ovarian endometrioma with a very high CA-125 level, moderately elevated CA 19-9, and CA 15-3 levels is presented. A 20 years old patient, complaining from pelvic pain, 5 cm adnexial mass was detected on left side. Biochemical examination was revealed very high CA-125 value (2556IU/ml, moderately elevated CA 19-9 (134IU/ml, and CA 15-3 (65IU/ml values. Laparoscopy was done and a ruptured ovarian endometrioma of 5 cm was seen during operation. After the total excision of the cyst, tumor markers fell rapidly. Very high CA-125 value, moderately elevated CA19-9, and CA 15-3 values can be seen in cases with ruptured endometrioma. In young patients, endometrioma must be considered firstly and laparoscopy should be applied instead of more invasive methods unless there was any finding or strong suspicion about malignancy.

  10. Modeling the contributions of Ca2+ flows to spontaneous Ca2+ oscillations and cortical spreading depression-triggered Ca2+ waves in astrocyte networks.

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    Bing Li

    Full Text Available Astrocytes participate in brain functions through Ca(2+ signals, including Ca(2+ waves and Ca(2+ oscillations. Currently the mechanisms of Ca(2+ signals in astrocytes are not fully clear. Here, we present a computational model to specify the relative contributions of different Ca(2+ flows between the extracellular space, the cytoplasm and the endoplasmic reticulum of astrocytes to the generation of spontaneous Ca(2+ oscillations (CASs and cortical spreading depression (CSD-triggered Ca(2+ waves (CSDCWs in a one-dimensional astrocyte network. This model shows that CASs depend primarily on Ca(2+ released from internal stores of astrocytes, and CSDCWs depend mainly on voltage-gated Ca(2+ influx. It predicts that voltage-gated Ca(2+ influx is able to generate Ca(2+ waves during the process of CSD even after depleting internal Ca(2+ stores. Furthermore, the model investigates the interactions between CASs and CSDCWs and shows that the pass of CSDCWs suppresses CASs, whereas CASs do not prevent the generation of CSDCWs. This work quantitatively analyzes the generation of astrocytic Ca(2+ signals and indicates different mechanisms underlying CSDCWs and non-CSDCWs. Research on the different types of Ca(2+ signals might help to understand the ways by which astrocytes participate in information processing in brain functions.

  11. Intercellular Ca2+ Waves: Mechanisms and Function

    Science.gov (United States)

    Sanderson, Michael J.

    2012-01-01

    Intercellular calcium (Ca2+) waves (ICWs) represent the propagation of increases in intracellular Ca2+ through a syncytium of cells and appear to be a fundamental mechanism for coordinating multicellular responses. ICWs occur in a wide diversity of cells and have been extensively studied in vitro. More recent studies focus on ICWs in vivo. ICWs are triggered by a variety of stimuli and involve the release of Ca2+ from internal stores. The propagation of ICWs predominately involves cell communication with internal messengers moving via gap junctions or extracellular messengers mediating paracrine signaling. ICWs appear to be important in both normal physiology as well as pathophysiological processes in a variety of organs and tissues including brain, liver, retina, cochlea, and vascular tissue. We review here the mechanisms of initiation and propagation of ICWs, the key intra- and extracellular messengers (inositol 1,4,5-trisphosphate and ATP) mediating ICWs, and the proposed physiological functions of ICWs. PMID:22811430

  12. Binding of Ca Ions by Paramecium caudatum

    Science.gov (United States)

    Naitoh, Yutaka; Yasumasu, Ikuo

    1967-01-01

    Binding of 45Ca by live Paramecium caudatum was determined under various external ionic conditions. It was found that calcium uptake was separable into at least two components, a rapid and a slow one. The rapid component was influenced by the presence of certain other ions in a manner which agrees with the law of mass action. It appears that an ion exchange system may be involved in a binding equilibrium established between Paramecium, Ca++, and certain other ions. K+, Rb+, and Ba++ in the equilibrium medium are among those ions which inhibit calcium uptake. It is proposed that liberation of Ca++ from binding sites on Paramecium by an exchange reaction with competing ions is the first step in the mechanism of ciliary reversal in the response to external application of these ions. PMID:6033587

  13. TIP27: a novel repressor of the nuclear orphan receptor TAK1/TR4

    Science.gov (United States)

    Nakajima, Takeshi; Fujino, Sanae; Nakanishi, Gen; Kim, Yong-Sik; Jetten, Anton M.

    2004-01-01

    The nuclear orphan receptor TAK1/TR4 functions as a positive as well as a negative regulator of transcription; however, little is known about the factors regulating or mediating its activity. Yeast two-hybrid analysis using the ligand-binding domain (LBD) of TAK1 as bait identified a novel TAK1-interacting protein, referred to as TIP27, which functions as a repressor of TAK1-mediated transactivation. TIP27 is a 27 kDa protein containing two zinc finger motifs. Mammalian two-hybrid analysis showed that TIP27 interacts specifically with TAK1 and not with several other nuclear receptors tested. The region between Asp39 and Lys79 of TIP27, referred to as TAK1-interaction domain (TID), is critical for its interaction with TAK1 while the TAK1-LBD from helix 3 until the C-terminus is required for the optimal interaction with TIP27. Pull-down assays demonstrated that the TIP27 physically interacts with TAK1 and supported the critical importance of the TID. Confocal microscopy showed that in the nucleus, TIP27 and TAK1 co-localize. TIP27 acts as a strong repressor of DR1-dependent transcriptional activation by TAK1. This repression does not involve the inhibition of TAK1 homodimerization or DR1 binding but may be due to an effect on co-activator recruitment by TAK1. Our results indicate that TIP27 functions as a TAK1-selective repressor. PMID:15302918

  14. As de basura. El tráfico ilegal de residuos

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    Ulderico Pesce

    2016-01-01

    Full Text Available Ulderico Pesce (Rivello, 1963, uno de los más significativos autores-narradores del teatro de narración de marcado contenido civil, vuelve a tratar en Asso di Monnezza el inmenso problema del lucrativo reciclaje de deshechos, sobre todo industriales, en la Italia actual. No es esta la primera vez que trata el tema, aunque en esta ocasión, partiendo de la visión directa del joven Nicola, se centra en la sangrante connivencia entre mafia, entidades públicas y los bajos fondos metropolitanos. En esta línea de denuncia social se mueven, de hecho, todos sus espectáculos de narración  en la línea del llamado teatro civile, como Storie di scorie. Il pericolo nucleare in Italia, sobre el peligro nuclear en tierras italianas; FIATo sul collo, sobre los 21 días de lucha de los trabajadores de la Fiat en Melfi; L'innaffiatore del cervello di Passannante: L'anarchico che cercò di uccidere Umberto I di Savoia, sobre la trágica figura histórica de Passannante, quien intentó asesinar al rey Umberto I en 1878; o Il Triangolo degli schiavi: I lavoratori clandestini in Italia, un áspero montaje sobre las pésimas condiciones laborales de algunos inmigrantes en tierras italianas, como muy bien indica el título.

  15. Del tráfico entre antropología y arte contemporáneo

    Directory of Open Access Journals (Sweden)

    Xavier Andrade

    2007-06-01

    Full Text Available Tomando los estudios sobre la economía de las drogas ilícitas como referente metodológico, el artículo discute preliminarmente la noción de “tráfico” en relación a cuatro aspectos del cruce de fronteras entre la antropología y el arte contemporáneo. 1. Transportar o movilizar bienes –en este caso, simbólicos, esto es básicamente ideas, categorías y conceptos pero también estrategias de apropiación y recontextualización pertinentes tanto a la etnografía como al arte contemporáneo. 2. Describir el carácter “contaminante” que tales bienes eventualmente adquieren cuando circulan en diferentes contextos (académico, gestión cultural, artes visuales, provocando por ello estrategias defensivas entre unos y otros. 3. Dar cuenta del carácter conflictivo de las micro-prácticas que se ponen a prueba durante el diálogo entre distintos saberes sancionados académicamente como campos o disciplinas. 4. Discutir los códigos de la ilegalidad que acompañan a la calidad de “traficante” y el capital simbólico que se deriva de ello.

  16. Mise-en-film da fotografia em três documentários brasileiros

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    Glaura Cardoso Vale

    2016-08-01

    Full Text Available Este artigo busca analisar, em três filmes da cinematografia brasileira recente, os modos como a fotografia, entendida como dispositivo de rememoração, é colocada em cena, compreendendo-a como rastro da presença de algo que já se tornou ausente. Pretende-se discutir como, ao solicitar imagens retiradas dos "álbuns de família", corrobora-se a construção de narrativas que dão a ver a biografia de homens e mulheres comuns, reais ou imaginados pelo documentário. Verificar, em metodologias distintas, o exercício político e estético do fazer cinematográfico que expõe as fragilidades e possíveis frustrações na reconstrução de algo longínquo (do ponto de vista do personagem e desconhecido (do ponto de vista dos autores, por mais presente materialmente que esteja a imagem no ato da rememoração.

  17. A Democracia em três dimensões: desiderativismo, realismo e sincretismo.

    Directory of Open Access Journals (Sweden)

    Reginaldo Teixeira Perez

    2015-05-01

    Full Text Available O objeto em foco neste artigo é a democracia contemporânea. Com o suporte metodológico da análise conceitual, define-se o fenômeno democrático a partir de três perspectivas: a normativa/desiderativa, a realista/descritiva e o que se poderia denominar de sincrética ou procedimental – uma tentativa de aproximação das duas anteriores. À primeira definição usar-se-á a ciência-matriz do Direito; à segunda, buscar-se-ão os subsídios na Economia; a terceira talvez seja bem designada como um artifício político. De um lado, temse a democracia política (com seus valores tendentes à igualdade; de outro, afigura-se a economia/sociedade de mercado (com seus interesses conducentes à diferença. Contrapostos, terão os dois projetos a sua interação colocada à prova. No final, tentar-se-á definir a democracia a partir de critérios mínimos – com o seu encaminhamento esteirado, necessariamente, no campo da política.

  18. Sob o signo do provisório: um diretor entre três cidades

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    Daniela Giovana Siqueira

    2017-01-01

    Full Text Available Este artigo é dedicado à análise do filme brasileiro A Vida Provisória (1968, do diretor Maurício Gomes Leite. A partir dele, estabelece-se uma discussão sobre a relação entre cinema e cidade. Para tanto, propõe-se deslocar a atenção do estudo da memória de sua especificidade temporal para a espacial, buscando entender como o cinema potencializa a discussão entre a cidade real e o discurso feito sobre as cidades. Para o exercício da análise fílmica elegem-se três principais categorias: a presença e os significados que reverberam a exposição das cidades do Rio de Janeiro, Belo Horizonte e Brasília na trama do filme; mimese entre diretor e personagem principal; e a tessitura do provisório na vida das personagens e a relação com o tempo histórico de realização do filme, em plena Ditadura Militar.

  19. Um mundo metafísico nos trópicos

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    Else Ribeiro Pires Vieira

    2008-04-01

    Full Text Available A fonte torna-se a estrela inatingível e pura que, sem se deixar contaminar, contamina, brilha para os artistas dos países da América Latina... ilumina os movimentos das mãos, mas ao mesmo tempo torna os artistas súditos do seu magnetismo superior.. Silviano Santiago, Uma literatura nos trópicos Afastando-me do discurso crítico em torno das influências e do caráter tributário a ele inerente, sobre o qual nos adverte Santiago já em ensaio de 1971,1 afastando-me, igualmente, de Polarizações hierarquizadas como fonte/alvo, situando-me na fronteira fluida do transformar de escritas/reescritas e nela privilegiando áreas de permeabilidade entre os discursos e o seu jogo relacional, ofereço, neste texto, reflexões em torno da poesia metafísica inglesa traduzida entre 1978 e 1991 na sua interseção com outras produções textuais brasileiras no período de início da abertura política, gestação e nascimento da Nova República2 . Traduzir a Inglaterra, ler o Brasil. O que se traduz? Quem se traduz? Por que se traduz? Como se traduz?

  20. Los árboles en la ganadería del trópico seco

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    J. M. Palma

    2005-01-01

    Full Text Available En el presente trabajo se resalta la presencia de árboles en los sistemas ganaderos del estado de Colima, México; lugar en donde las características climáticas predominantes son de trópico seco. Se enfatiza la importancia de la vegetación arbórea nativa, tanto de especies leguminosas como no leguminosas, manejadas ya sea en forma empírica como científica de algunos sistemas silvopastoriles (SSP presentes en la región. Destacan las características nutricionales tanto del follaje como de los frutos, con la descripción de algunos SSP; entre ellos, el manejo del cocotero en la región costera, los árboles en los potreros, experiencias con el manejo de los bancos de proteína, la utilización de cercas vivas y algunas asociaciones forrajeras. Destaca la diversidad de árboles susceptibles de ser incorporados en el desarrollo de sistemas silvopastoriles, así como la necesidad de generar una cultura para la conservación y multiplicación de estas especies, sobre todo en ambientes en donde la deforestación y la erosión son una amenaza.

  1. Meningiomas múltiplos e neurofibromatose: relato de três casos

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    Mauro A. Oliveira

    1993-06-01

    Full Text Available Meningiomas múltiplos «verdadeiros" geralmente não constituem entidade patológica específica. Em geral, associam-se a neurofibromatose. As clássicas lesões externas, descritas por von Recklinghausen, associadas à neurofibromatose, podem não estar presentes. Isto poderia ser possível devido à penetrância variável de aberrações cromossômicas ligadas ao cromossomo 22. Estudos moleculares desses tumores confirmam esta hipótese. Em nossa série de 108 pacientes com diagnóstico de meningioma intarcraniano, apenas três eram múltiplos. Apenas em um caso, estigmas externos de neurofibromatose foram encontrados. Os dois casos que não apresentavam qualquer estigma foram considerados como meningiomas múltiplos «verdadeiros". Na ausência de manifestações cutâneas habitualmente associadas à neurofibromatose, é extremamente difícil distinguir meningiomas múltiplos associados a neurofibromatose dos assim chamados meningiomas múltiplos verdadeiros. Em nossa opinião não há justificativa para se considerar meningiomas múltiplos como entidade patológica independente.

  2. Dor-desprazer-trabalho docente: como desfazer essa tríade

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    Maria Elizabeth Barros de Barros

    2007-12-01

    Full Text Available O artigo apresenta a experiência do Programa de Formação e Investigação Sobre a Saúde e o Trabalho (PFST de docentes de escolas públicas, desenvolvido na Universidade Federal do Espírito Santo. Trata da problemática da saúde do conjunto de docentes que trabalham nas escolas públicas do município da Serra/ES, visando a desfazer a tríade dor-desprazer-trabalho docente, vivida de forma naturalizada pelo coletivo de docentes. Pretende avançar na compreensão das relações saúde-trabalho nas escolas e investigar as estratégias utilizadas por esses professores para resistirem às tentativas de desqualificação do trabalho docente. Afirma a possibilidade de se abrirem espaços de discussão no cotidiano dos docentes para que a luta pela saúde se constitua em redes de cooperação entre sujeitos e escolas, inaugurando-se outras formas de atuação desses estabelecimentos. Com esse objetivo, propõe uma metodologia de trabalho que tem se pautado na abordagem ergológica, construída na Universidade de Provença, em AIX/ França.

  3. Três lições de filosofia da educação

    Directory of Open Access Journals (Sweden)

    Kohan Walter Omar

    2003-01-01

    Full Text Available O presente trabalho busca pensar o valorde O mestre ignorante como exercício de filosofia da educação, em particular contrapondo-o a um modo, dominante, de exercer esse saber no seio de nossas instituições. Trata-se de uma história singular, pela qual todo mestre pode se perguntar por que e para que ensina; e, o que é ainda mais importante, pela qual pode questionar-se que diabos está fazendo consigo mesmo e com os outros, a cada vez que se veste de mestre em uma sala de aula. Depreendemos, desse exercício, três lições: a o mais natural, evidente e aceito socialmente acaba sendo, filosoficamente, o mais problemático; b somente pelo paradoxo, entranhados no lodo paradoxal, podemos encontrar algum sentido na educação; c só há uma educação que vale a pena: a que emancipa (sem emancipar. Quem não deixa que os(as outros(as se emancipem embrutece.

  4. Três mulheres no nazismo: Reflexões sobre as fontes do comportamento moral

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    Maria Claudia Coelho

    2009-07-01

    Full Text Available Este trabalho discute as fontes do comportamento moral. Seu ponto de partida é a crítica feita à sociologia da moral durkheimiana por Zygmunt Bauman, que problematiza os pressupostos da origem social da moral e sua natureza coercitiva. Bauman contrapropõe, com base nas obras de Hannah Arendt e Emmanuel Lévinas, uma concepção de moralidade cujo núcleo é a responsabilidade para com o outro. A discussão é empreendida com base em três filmes que retratam cada qual a trajetória de uma mulher durante o nazismo: Traudl Junge, Sophie Scholl e Leni Riefenstahl. O eixo da análise é o modo como responsabilidade, culpa e consciência se entrelaçam em suas trajetórias. This article discusses the sources of moral behaviour. Its point of departure is Zygmunt Bauman’s criticism of the Durkheimian sociology of morality, challenging the assumptions of the social origin of morality and its coercive nature. Drawing on Hannah Arendt’s and Emmanuel Levinas’ works, Bauman counter-proposes a concept of morality based on responsibility toward each other. The discussion is then developed in relation to three fi lms that portray, each in their own way, the experience of a woman during the Nazi regime: Traudl Junge, Sophie Scholl and Leni Riefenstahl. The analysis is centred on how responsibility, guilt and conscience intertwine in the course of those lives.

  5. A cobertura de ciência em três jornais paraenses: um estudo longitudinal

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    Vanessa Brasil de Carvalho

    Full Text Available Resumo Neste artigo, apresentamos uma proposta metodológica visando realizar um estudo longitudinal que fornecesse um panorama da cobertura de ciência em 130 anos, por três jornais importantes do Estado do Pará: A Província do Pará (1876-2002, Folha do Norte (1896-1974 e O Liberal (1946-atual. Analisamos um período de dois meses a cada dez anos, de 1876 a 2006, em cada jornal selecionado, de maneira que obtivemos um olhar em longo prazo que perpassou pela história dos periódicos e, portanto, por grande parte da história da imprensa no Pará e na Amazônia. Utilizamos análise de conteúdo e análise de frames da Mídia. Nossos dados mostram que houve ênfase na pesquisa em saúde, destaque para a ciência nacional e preocupação em contextualizar os fatos científicos. Controvérsias e incertezas científicas ganharam pouco espaço nos jornais analisados.

  6. Spontaneous quaternary and tertiary T-R transitions of human hemoglobin in molecular dynamics simulation.

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    Jochen S Hub

    2010-05-01

    Full Text Available We present molecular dynamics simulations of unliganded human hemoglobin (Hb A under physiological conditions, starting from the R, R2, and T state. The simulations were carried out with protonated and deprotonated HC3 histidines His(beta146, and they sum up to a total length of 5.6 micros. We observe spontaneous and reproducible T-->R quaternary transitions of the Hb tetramer and tertiary transitions of the alpha and beta subunits, as detected from principal component projections, from an RMSD measure, and from rigid body rotation analysis. The simulations reveal a marked asymmetry between the alpha and beta subunits. Using the mutual information as correlation measure, we find that the beta subunits are substantially more strongly linked to the quaternary transition than the alpha subunits. In addition, the tertiary populations of the alpha and beta subunits differ substantially, with the beta subunits showing a tendency towards R, and the alpha subunits showing a tendency towards T. Based on the simulation results, we present a transition pathway for coupled quaternary and tertiary transitions between the R and T conformations of Hb.

  7. O TRABALHO EM ANDRÉ GORZ: três reflexões, uma problemática

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    Henrique Amorim

    Full Text Available Há aproximadamente dez anos André Gorz morreu, deixando ideias estimulantes sobre os limites e a necessidade de superação ou desconstrução da sociedade capitalista. Sempre atento às transformações sociais, Gorz chegou, em certos momentos, a sintetizar tudo que havia de mais profundo e crítico na teoria social. Entretanto, é possível observar, em sua obra, uma oscilação em relação ao lugar social que o trabalho ocupa. O lugar do trabalho parece mudar em função do desenvolvimento das forças produtivas. Assim, a análise do movimento social do trabalho na sociedade capitalista, realizada por Gorz, permanece prisioneira de uma determinação tecnológica e de um conceito de trabalho limitado ao universo produtivo das qualificações profissionais. Neste artigo, tenho como objetivo problematizar a concepção de trabalho exposta por Gorz em três de suas principais obras: Estratégia Operária e Neocapitalismo (1964, Adeus ao Proletariado (1980 e O Imaterial (2003, demonstrando como a teoria social e as alternativas políticas desenvolvidas por Gorz mudam vis-à-vis o lugar social ocupado pelo trabalho nessas obras.

  8. La experiencia del embarazo en el tránsito de la adolescencia

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    Alicia Genolet

    2004-01-01

    Full Text Available El presente artículo expone el comportamiento estadístico de las madres adolescentes atendidas en su parto en el Servicio de Maternidad del Hospital «San Roque» (Paraná, Entre Ríos durante 1997, 1998 y 1999, y las vivencias y representaciones que portan un grupo de adolescentes (madres y no madres con relación a la maternidad y la anticoconcepción. El estudio estadístico se basó en una muestra de 890 fichas de parto, que fueron procesadas con el software SPSS. Por otra parte, la interpretación de los datos cualitativos permite construir las siguientes conceptualizaciones: Las adolescentes estudiadas sufren condiciones que hablan de una triple vulnerabilidad: por ser mujeres, por ser pobres y por ser adolescentes. La adolescencia constituye un momento de vulnerabilidad de los sujetos en su tránsito a la adultez, que cobra una significación especial cuando tiene lugar en situaciones de riesgo, fragilidad y precariedad en los vínculos relacionales, familiares y sociales. Las condiciones de vulnerabilidad y pobreza refuerzan la condición subordinada del género femenino: ejercicio de roles tradicionales (crianza de los hijos, trabajo doméstico, exclusión de instancias de participación social y educativas.

  9. Evaluation of Llaima volcano activities for localization and classification of LP, VT and TR events

    Science.gov (United States)

    Dehghan Firoozabadi, Ali; Seguel, Fabian; Soto, Ismael; Guevara, David; Huenupan, Fernando; Curilem, Millaray; Franco, Luis

    2017-09-01

    Evaluation of seismic signals is one of the most important research topics on Volcanology. Volcanoes have daily activity; therefore, high speed evaluation of recorded signals is a challenge for improving the study of the natural phenomena occurring inside these natural formations. The aim of this paper is the evaluation (denoising, localization and classification) and analysis of Llaima volcano activities, one of the most actives volcanoes in South America. Different already proposed methods, such as, Butterworth, Spectral Subtraction (SS) and Wiener Filter (WF) are compared to the proposed Modified Spectral Subtraction (MSS) and Modified Wiener Filter (MWF) to find the best method for denoising the volcano signals. Then, event localization based on received signals of volcano is performed. In this step, Time Delay Estimation (TDE)-based method is used on data acquired from 3 mechanical sensors located in the volcano area. The proposed method is used to estimate the area for event location. The proposed denoising methods make the starting point for the event more evident to increase the localization accuracy for events where the starting point is difficult to find. In the last step, a method based on the novel DNN technique is proposed to classify the three main events occurring in the Llaima volcano (TR (Tremor), LP (Long Period) and VT (Volcano Tectonic)).

  10. Highly Conductive Anion-Exchange Membranes from Microporous Tröger's Base Polymers.

    Science.gov (United States)

    Yang, Zhengjin; Guo, Rui; Malpass-Evans, Richard; Carta, Mariolino; McKeown, Neil B; Guiver, Michael D; Wu, Liang; Xu, Tongwen

    2016-09-12

    The development of polymeric anion-exchange membranes (AEMs) combining high ion conductivity and long-term stability is a major challenge for materials chemistry. AEMs with regularly distributed fixed cationic groups, based on the formation of microporous polymers containing the V-shape rigid Tröger's base units, are reported for the first time. Despite their simple preparation, which involves only two synthetic steps using commercially available precursors, the polymers provide AEMs with exceptional hydroxide conductivity at relatively low ion-exchange capacity, as well as a high swelling resistance and chemical stability. An unprecedented hydroxide conductivity of 164.4 mS cm(-1) is obtained at a relatively a low ion-exchange capacity of 0.82 mmol g(-1) under optimal operating conditions. The exceptional anion conductivity appears related to the intrinsic microporosity of the charged polymer matrix, which facilitates rapid anion transport. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  11. BlastX Result : TrEMBL - AcEST | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available .nbdc00839-003 Description of data contents Results of the blastx search of Adiantum capillus-veneris EST (A...nd an Adiantum capillus-veneris EST sequence Report Results of blastx search against the UniProtKB/TrEMBL (r

  12. La tríada oscura de la personalidad: maquiavelismo, narcisismo y psicopatía. Una mirada evolutiva

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    Fernando Renee González Moraga

    2015-08-01

    Full Text Available Se aborda la denominada Tríada Oscura de la Personalidad -Tríada- (maquiavelismo, narcisismo subclínico y psicopatía subclínica desde una mirada evolutiva. El objetivo de esta investigación es revisar la evidencia que han presentado los teóricos evolucionistas sobre la Tríada, desde un acercamiento a la mirada evolutiva. Para esto, se indaga en las principales características de cada uno de estos rasgos, los instrumentos que se han utilizado para evaluarlos y las principales áreas en donde se han estudiado. La mirada evolutiva plantea que los rasgos de la Tríada son dimensionales y varían de acuerdo con las diversas estrategias que utilizan los sujetos para adaptarse a las características socioambientales en las que se desarrollan. Estos rasgos se caracterizan por presentar violencia psicológica, inhibición moral, manipulación, baja amabilidad, insensibilidad, egoísmo y dificultad para mentalizar en contextos de interacción interpersonal y social.

  13. Unveiling the piezoelectric nature of polar α-phase P(VDF-TrFE) at quasi-two-dimensional limit.

    Science.gov (United States)

    Qian, Jun; Jiang, Sai; Wang, Qijing; Zheng, Shushu; Guo, Shuya; Yi, Chang; Wang, Jianpu; Wang, Xinran; Tsukagoshi, Kazuhito; Shi, Yi; Li, Yun

    2018-01-11

    Piezoelectric response of P(VDF-TrFE), which is modulated by the dipole density due to the polarization switching on applying an electric field, allows it act as the fundamental components for electromechanical systems. As proposed since the 1970s, its polar α-phase is supposed to yield an enhanced piezoelectric activity. However, its experimental verification has never been reported, hampered by a substantial challenge for the achievement of a smooth, neat α-phase film. Here, we prepare ultrathin crystalline α-phase P(VDF-TrFE) films on the AlO x /Al-coated SiO 2 /Si substrates via a solution-based approach at room temperature. Thus, we unveil the piezoelectric nature of the polar α-phase P(VDF-TrFE) at a quasi-two-dimensional limit. The obtained values of the relative morphological deformation, the local effective piezoelectric coefficient, and the electric field-induced strain reach up to 37 pm, -46.4 pm V -1 , and 4.1%, respectively. Such a robust piezoelectric response is even higher than that of the β-phase. Besides, the evolution of piezoelectricity, which is related to the piezoelectric properties of two polarization states, is also studied. Our work can enable the exploration of the prospective applications of polar α-phase P(VDF-TrFE) films.

  14. Variability among Research Diagnostic Interview Instruments in the Application of "DSM-IV-TR" Criteria for Pediatric Bipolar Disorder

    Science.gov (United States)

    Galanter, Cathryn A.; Hundt, Stephanie R.; Goyal, Parag; Le, Jenna; Fisher, Prudence W.

    2012-01-01

    Objective: The "DSM-IV-TR "criteria for a manic episode and bipolar disorder (BD) were developed for adults but are used for children. The manner in which clinicians and researchers interpret these criteria may have contributed to the increase in BD diagnoses given to youth. Research interviews are designed to improve diagnostic reliability and…

  15. Structural and Functional Characterization of Nrf2 Degradation by the Glycogen Synthase Kinase 3/β-TrCP Axis

    Science.gov (United States)

    Rada, Patricia; Rojo, Ana I.; Evrard-Todeschi, Nathalie; Innamorato, Nadia G.; Cotte, Axelle; Jaworski, Tomasz; Tobón-Velasco, Julio C.; Devijver, Herman; García-Mayoral, María Flor; Van Leuven, Fred; Hayes, John D.

    2012-01-01

    The transcription factor NF-E2-related factor 2 (Nrf2) is a master regulator of a genetic program, termed the phase 2 response, that controls redox homeostasis and participates in multiple aspects of physiology and pathology. Nrf2 protein stability is regulated by two E3 ubiquitin ligase adaptors, Keap1 and β-TrCP, the latter of which was only recently reported. Here, two-dimensional (2D) gel electrophoresis and site-directed mutagenesis allowed us to identify two serines of Nrf2 that are phosphorylated by glycogen synthase kinase 3β (GSK-3β) in the sequence DSGISL. Nuclear magnetic resonance studies defined key residues of this phosphosequence involved in docking to the WD40 propeller of β-TrCP, through electrostatic and hydrophobic interactions. We also identified three arginine residues of β-TrCP that participate in Nrf2 docking. Intraperitoneal injection of the GSK-3 inhibitor SB216763 led to increased Nrf2 and heme oxygenase-1 levels in liver and hippocampus. Moreover, mice with hippocampal absence of GSK-3β exhibited increased levels of Nrf2 and phase 2 gene products, reduced glutathione, and decreased levels of carbonylated proteins and malondialdehyde. This study establishes the structural parameters of the interaction of Nrf2 with the GSK-3/β-TrCP axis and its functional relevance in the regulation of Nrf2 by the signaling pathways that impinge on GSK-3. PMID:22751928

  16. Dimensión social del tráfico ilícito de bienes culturales

    OpenAIRE

    García, Tania

    2012-01-01

    Cadena de involucrados en el TIBC. Huaqueros y coleccionistas. Régimen de tenencia de tierra y sitios arqueológicos. Colecciones arqueológicas registradas por el INPC. Blanqueo de bienes. Seguridad ciudadana y tráfico ilícito de bienes culturales. Universidad Tecnológica de El Salvador

  17. Perfiles resilientes y calidad de vida en personas con discapacidad sobrevenida por accidentes de tráfico

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    Raquel Suriá Martínez

    2015-09-01

    Conclusión: Esto sugiere la necesidad de profundizar en el conocimiento de la resiliencia y en el diseño de programas que incrementen la calidad de vida de las personas con discapacidad tras un accidente de tráfico.

  18. A atualidade dos acidentes de trânsito na era da velocidade: uma visão geral

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    Marín Letícia

    2000-01-01

    Full Text Available Este artigo focaliza, numa perspectiva interdisciplinar, os estudos sobre acidentes de trânsito em escala nacional e internacional. Ele começa analisando o aumento da produção e consumo de veículos motorizados em todo o mundo e as transformações sociais que esse fato acarretou. Atenção especial é dada à degradação do meio ambiente urbano e ao enorme custo social representado pelos acidentes de trânsito. Em seguida, é apresentado um panorama epidemiológico sobre as vítimas do trânsito. A relação entre personalidade e acidente de trânsito mereceu atenção especial, principalmente no que se refere ao comportamento infrator e ao consumo de bebidas alcoólicas e de outras drogas. O artigo conclui enfatizando a necessidade de o Estado implementar políticas públicas específicas consistentes, a fim de se poder controlar o problema.

  19. Mapping the manuals of madness: Comparing the ICD-10 and DSM-IV-TR using a network approach.

    Science.gov (United States)

    Tio, Pia; Epskamp, Sacha; Noordhof, Arjen; Borsboom, Denny

    2016-12-01

    The International Classification of Diseases and Related Health Problems (ICD) and the Diagnostic and Statistical Manual of Mental Disorders (DSM) represent dominant approaches to diagnosis of mental disorders. However, it is unclear how these alternative systems relate to each other when taking into account the symptoms that make up the disorders. This study uses a network approach to investigate the overlap in structure between diagnostic networks pertaining to ICD-10 and DSM-IV-TR. Networks are constructed by representing individual symptoms as nodes, and connecting nodes whenever the corresponding symptoms feature as diagnostic criteria for the same mental disorder. Results indicate that, relative to the DSM-IV-TR network, the ICD-10 network contains (a) more nodes, (b) lower level of clustering, and (c) a higher level of connectivity. Both networks show features of a small world, and have similar (of "the same") high centrality nodes. Comparison to empirical data indicates that the DSM-IV-TR network structure follows comorbidity rates more closely than the ICD-10 network structure. We conclude that, despite their apparent likeness, ICD-10 and DSM-IV-TR harbour important structural differences, and that both may be improved by matching diagnostic categories more closely to empirically determined network structures. Copyright © 2016 John Wiley & Sons, Ltd. Copyright © 2016 John Wiley & Sons, Ltd.

  20. Acidentes de trânsito no Brasil de 1998 a 2010: muitas mudanças e poucos resultados

    Directory of Open Access Journals (Sweden)

    Giancarlo Bacchieri

    2011-10-01

    Full Text Available O artigo descreve a situação dos acidentes de trânsito no Brasil, desde a implementação do Código de Trânsito Brasileiro de 1998 até o ano de 2010. Foi realizada análise dos principais trabalhos científicos e publicações não acadêmicas nacionais. A revisão de literatura incluiu periódicos indexados, não indexados, relatórios técnicos, busca específica por autores, referências bibliográficas de artigos e contato com pesquisadores. Os principais problemas do trânsito brasileiro identificados foram aumento do número absoluto de mortos e das taxas de mortalidade, ampliação da frota de motocicletas e o uso de álcool. Foram identificados autores influentes e ilhas de produção de conhecimento nas áreas pesquisadas. Os autores apresentam algumas possíveis soluções e sugerem que o poder público não tem assumido a responsabilidade que lhe cabe no controle e redução dos acidentes de trânsito.

  1. TR{alpha}- and TSH-mRNA levels after temporal exposition with methimazole in zebrafish, Danio rerio

    Energy Technology Data Exchange (ETDEWEB)

    Schulz, A.E.I.; Stocker, A.; Hollosi, L.; Schramm, K.W. [Inst. of Ecological Chemistry, GSF - National Research Center for Environment and Health (Germany)

    2004-09-15

    The group of dioxin and dioxin-like substances are highly persistent in the environment. There are evidences from present investigations that a variety of substances are capable of disrupting the endocrine system in the aquatic environment. These substances are called endocrine disruptors. Dioxin and related compounds can act as endocrine disruptors. Aquatic animals like amphibian and fish are especially affected of the impact of these compounds. Investigations concerned so far in particular the domain of reproduction biology and the thyroid axis especially. Recent investigations showed that the TR{alpha}-mRNA level change after a short temporal expression with T3, methimazole and amiodarone. The objective of the project is to identify effects of thyroid endocrine disruptors on the regulation of gene expression of the thyroid receptors TR{alpha}a, TR{beta} and thyroid stimulating hormone TSH and associated effects on other system. In preliminary studies the effects of the drug methimazole as model substance on gene expression of TR{alpha} and TSH were investigated. Methimazole is an inhibitor of the thyroid peroxidase so that the formation of thyroid hormones is disrupted.

  2. [CD4(+) CD25(+) Tr cells and transcription factor Foxp3 in the naturally tolerance of rat liver transplantation].

    Science.gov (United States)

    Zhang, Feng; Lv, Ling; Pu, Li-Yong; Li, Xiang-Cheng; Yao, Ai-Hua; Zhang, Wei; Yu, Yue; Wang, Xue-Hao

    2006-11-01

    To investigate the role of intrahepatic CD4(+)CD25(+) T regulatory cells and Foxp3 gene in the natural tolerance in rat liver transplantation. The orthotopic liver transplantation models of inbred rats (LEW and DA rats) were established with double-sleeve technique and the models were divided into two groups: tolerance group (TOL group, LEW-to-DA) and rejection group (REJ group, DA-to-LEW). The intrahepatic lymphocytes from each group were isolated by using density gradient centrifugation. CD4(+)CD25(+) T cells were isolated by magic cell sorting system (MACS) and identified by flow cytometry (FCM). CD4(+)CD25(+) Tr cells suppression on the proliferation of CD4(+)CD25(-) T effector cells were analyzed by cell proliferation assay in vitro. Western blot was used to detect Scurfin protein expression of CD4(+)CD25(+) Tr cells. CD4(+)CD25(+) Tr cells developed significantly greater in the TOL group than in the REJ group. In vitro, the spleen cells from LEW rats can irritate the proliferation of CD4(+)CD25(+) T cells more obviously than the syngeneic spleen cells. CD4(+)CD25(+) T cells could suppress the proliferation of CD4(+)CD25(-) T cells, but the inhibition was reversed by exogenous IL-2 (200 U/ml). The immune suppression function of CD4(+)CD25(+) Tr cell, mediated by Foxp3 gene, is one of the mechanisms in liver transplantation tolerance.

  3. Identifying the Perceptual Learning Styles of Foreign Language Learners: A Comparative Study in TR63 (Osmaniye, Hatay and Kahramanmaras)

    Science.gov (United States)

    Kurt, Mehmet; Bilginer, Hayriye

    2016-01-01

    In the globalizing world economy, for the realization of international trade is increasing the need for foreign language learning. TR63 (Kahramanmaras, Osmaniye and Hatay) region is increasing its export every day. Besides these advances, interest is awakening in foreign language education among the region. In preparing syllabus for this kind of…

  4. TR(beta)1 protein is preferentially expressed in the pericentral zone of rat liver and exhibits marked diurnal variation

    NARCIS (Netherlands)

    Zandieh Doulabi, B.; ter Platvoet-Schiphorst, M.; van Beeren, H.C.; Labruyëre, W.T.; Lamers, W.H.; Fliers, E.; Bakker, O.; Wiersinga, W.M.

    2002-01-01

    TR(beta)1 protein is preferentially expressed in the pericentral zone of rat liver and exhibits marked diurnal variation. Zandieh Doulabi B, Platvoet-ter Schiphorst M, van Beeren HC, Labruyere WT, Lamers WH, Fliers E, Bakker O, Wiersinga WM. Department of Endocrinology and Metabolism, Academic

  5. El delito culposo en tránsito y la embriaguez

    OpenAIRE

    Obando Luna, Oscar Artemio

    2016-01-01

    Al ser el Ecuador un Estado constitucional de derechos y Justicia como lo señala el artículo. 1 de la Constitución del Ecuador, es necesario hacer varias puntualizaciones y citar algunas disposiciones constitucionales que son importantes justamente para el análisis y justificación del tema. “(…) Art. 75 de la Constitución establece que toda persona tiene derecho a la tutela efectiva, imparcial y expedita de sus derechos e intereses, con sujeción a los principios de inmediación, y en ningún ca...

  6. Ca2+ extrusion via Na+-Ca2+ exchangers in rat odontoblasts.

    Science.gov (United States)

    Tsumura, Maki; Okumura, Reijiro; Tatsuyama, Shoko; Ichikawa, Hideki; Muramatsu, Takashi; Matsuda, Toshio; Baba, Akemichi; Suzuki, Keiko; Kajiya, Hiroshi; Sahara, Yoshinori; Tokuda, Masayuki; Momose, Yasunori; Tazaki, Masakazu; Shimono, Masaki; Shibukawa, Yoshiyuki

    2010-04-01

    Intracellular Ca(2+) is essential to many signal transduction pathways, and its level is tightly regulated by the Ca(2+) extrusion system in the plasma membrane, which includes the Na(+)-Ca(2+) exchanger (NCX). Although expression of NCX1 isoforms has been demonstrated in odontoblasts, the detailed properties of NCX remain to be clarified. In this study, we investigated localization and ion-transporting/pharmacologic properties of NCX isoforms in rat odontoblasts. We characterized both the reverse and forward modes of NCX activity in odontoblasts in a dental pulp slice preparation. Ca(2+) influx by reverse NCX activity was measured by fura-2 fluorescence. Ca(2+) efflux by forward NCX activity elicited inward Na(+) current as measured by perforated-patch clamp recording. For immunohistochemical analysis, cryostat sections of incisors were incubated with antibodies against NCX. Immunohistochemical observation revealed localization of NCX1 and NCX3 in the distal membrane of odontoblasts. Inward currents by forward NCX activity showed dependence on external Na(+). Fura-2 fluorescence measurement revealed that Ca(2+) influx by reverse NCX activity depended on extracellular Ca(2+) concentration, and that this influx was blocked by NCX inhibitor KB-R7943 in a concentration-dependent manner. However, Ca(2+) influx by NCX showed a slight sensitivity to SEA0400 (a potent NCX1 inhibitor), indicating that expression potencies in odontoblasts were NCX3 > NCX1. These results suggest that odontoblasts express NCX1 and NCX3 at the distal membrane, and that these isoforms play an important role in the Ca(2+) extrusion system as well as in the directional Ca(2+) transport pathway from the circulation to the dentin-mineralizing front. Copyright (c) 2010 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.

  7. Growth rate effects on Mg/Ca and Sr/Ca ratios constrained by belemnite calcite

    Science.gov (United States)

    Vinzenz Ullmann, Clemens

    2016-04-01

    Multiple temperature proxies from single species are important to achieve robust palaeotemperature estimates. Besides the commonly employed oxygen isotope thermometer, also Mg/Ca and Sr/Ca ratios perform well as proxies for calcification temperature in the shells of some species. While salinity changes affect the ratios of earth alkaline elements much less than the δ18O thermometer, metabolic effects may exert a strong control on the expression of element ratios. Such effects are hard to study because biomineralization experiments have to overcome large intraspecific variability and can hardly ever isolate the controls of a single parameter on shell geochemistry. The unique geometry of the belemnite rostrum constitutes an exception to this rule. Its shape, large size, and the visibility of growth increments as bands enable the analysis of multiple, correlatable, high resolution geochemical profiles in a single fossil. The effects of the growth rate variability amongst these profiles on Mg/Ca and Sr/Ca ratios has been tested here. Within a specimen of Passaloteuthis bisulcata (Early Toarcian, Cleveland Basin, UK), Mg/Ca and Sr/Ca data were obtained from four profiles. With respect to growth rate in the first profile, which was taken as a reference, the relative growth rates in the remaining three profiles varied by a factor of 0.9 to 2.7. Results suggest that relative growth rate is linearly correlated with Mg/Ca and Sr/Ca, with a decrease of Mg/Ca by 8 % and increase of Sr/Ca by 6 % per 100 % increase in relative growth rate. The observed trends are consistent with abiogenic precipitation experiments and suggest that crystal precipitation rate exerts a significant, predictable control on the element distribution in biogenic calcite.

  8. The School Anxiety Scale-Teacher Report (SAS-TR): translation and psychometric properties of the Iranian version.

    Science.gov (United States)

    Hajiamini, Zahra; Mohamadi, Ashraf; Ebadi, Abbas; Fathi- Ashtiani, Ali; Tavousi, Mahmoud; Montazeri, Ali

    2012-07-18

    The School Anxiety Scale-Teacher Report (SAS-TR) was designed to assess anxiety in children at school. The SAS-TR is a proxy rated measure and could assess social anxiety, generalized anxiety and also gives a total anxiety score. This study aimed to translate and validate the SAS-TR in Iran. The translation and cultural adaptation of the original questionnaire were carried out in accordance with the published guidelines. A sample of students participated in the study. Reliability was estimated using internal consistency and test-retest analysis. Validity was assessed using content validity. The factor structure of the questionnaire was extracted by performing both exploratory and confirmatory factor analyses. In all 200 elementary students aged 6 to 10 years were studied. Considering the recommended cut-off values, overall the prevalence of high anxiety condition in elementary students was found to be 21 %. Cronbach's alpha coefficient for the Iranian SAS-TR was 0.92 and intraclass correlation coefficient (ICC) was found to be 0.81. The principal component analysis indicated a two-factor structure for the questionnaire (generalized and social anxiety) that jointly accounted for 55.3 % of variances observed. The confirmatory factory analysis also indicated a good fit to the data for the two-latent structure of the questionnaire. In general the findings suggest that the Iranian version of SAS-TR has satisfactory reliability, and validity for measuring anxiety in 6 to 10 years old children in Iran. It is simple and easy to use and now can be applied in future studies.

  9. The School Anxiety Scale-Teacher Report (SAS-TR: translation and psychometric properties of the Iranian version

    Directory of Open Access Journals (Sweden)

    Hajiamini Zahra

    2012-07-01

    Full Text Available Abstract Background The School Anxiety Scale-Teacher Report (SAS-TR was designed to assess anxiety in children at school. The SAS-TR is a proxy rated measure and could assess social anxiety, generalized anxiety and also gives a total anxiety score. This study aimed to translate and validate the SAS-TR in Iran. Methods The translation and cultural adaptation of the original questionnaire were carried out in accordance with the published guidelines. A sample of students participated in the study. Reliability was estimated using internal consistency and test-retest analysis. Validity was assessed using content validity. The factor structure of the questionnaire was extracted by performing both exploratory and confirmatory factor analyses. Results In all 200 elementary students aged 6 to 10 years were studied. Considering the recommended cut-off values, overall the prevalence of high anxiety condition in elementary students was found to be 21 %. Cronbach's alpha coefficient for the Iranian SAS-TR was 0.92 and intraclass correlation coefficient (ICC was found to be 0.81. The principal component analysis indicated a two-factor structure for the questionnaire (generalized and social anxiety that jointly accounted for 55.3 % of variances observed. The confirmatory factory analysis also indicated a good fit to the data for the two-latent structure of the questionnaire. Conclusion In general the findings suggest that the Iranian version of SAS-TR has satisfactory reliability, and validity for measuring anxiety in 6 to 10 years old children in Iran. It is simple and easy to use and now can be applied in future studies.

  10. Enhanced noradrenergic axon regeneration into schwann cell-filled PVDF-TrFE conduits after complete spinal cord transection.

    Science.gov (United States)

    Lee, Yee-Shuan; Wu, Siliang; Arinzeh, Treena Livingston; Bunge, Mary Bartlett

    2017-02-01

    Schwann cell (SC) transplantation has been utilized for spinal cord repair and demonstrated to be a promising therapeutic strategy. In this study, we investigated the feasibility of combining SC transplantation with novel conduits to bridge the completely transected adult rat spinal cord. This is the first and initial study to evaluate the potential of using a fibrous piezoelectric polyvinylidene fluoride trifluoroethylene (PVDF-TrFE) conduit with SCs for spinal cord repair. PVDF-TrFE has been shown to enhance neurite growth in vitro and peripheral nerve repair in vivo. In this study, SCs adhered and proliferated when seeded onto PVDF-TrFE scaffolds in vitro. SCs and PVDF-TrFE conduits, consisting of random or aligned fibrous inner walls, were transplanted into transected rat spinal cords for 3 weeks to examine early repair. Glial fibrillary acidic protein (GFAP)+ astrocyte processes and GFP (green fluorescent protein)-SCs were interdigitated at both rostral and caudal spinal cord/SC transplant interfaces in both types of conduits, indicative of permissivity to axon growth. More noradrenergic/DβH+ (dopamine-beta-hydroxylase) brainstem axons regenerated across the transplant when greater numbers of GFAP+ astrocyte processes were present. Aligned conduits promoted extension of DβH+ axons and GFAP+ processes farther into the transplant than random conduits. Sensory CGRP+ (calcitonin gene-related peptide) axons were present at the caudal interface. Blood vessels formed throughout the transplant in both conduits. This study demonstrates that PVDF-TrFE conduits harboring SCs are promising for spinal cord repair and deserve further investigation. Biotechnol. Bioeng. 2017;114: 444-456. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

  11. Effects of Traumeel (Tr14 on Exercise-Induced Muscle Damage Response in Healthy Subjects: A Double-Blind RCT

    Directory of Open Access Journals (Sweden)

    Kerstin Muders

    2016-01-01

    Full Text Available The present double-blind, randomized, placebo-controlled clinical trial intended to test whether ingestion of a natural combination medicine (Tr14 tablets affects serum muscle damage and inflammatory immune response after downhill running. 96 male subjects received Tr14 tablets, which consist of 14 diluted biological and mineral components, or a placebo for 72 h after the exercise test, respectively. Changes in postexercise levels of various serum muscle damage and immunological markers were investigated. The area under the curve with respect to the increase (AUCi of perceived pain score and creatine kinase (CK were defined as primary outcome measures. While for CK the p value of the difference between the two groups is borderline, the pain score and muscle strength were not statistically significant. However, a trend towards lower levels of muscle damage (CK, p=0.05; LDH, p=0.06 in the Tr14 group was shown. Less pronounced lymphopenia (p=0.02, a trend towards a lower expression of CD69 count (p=0.07, and antigen-stimulated ICAM-1 (p=0.01 were found in the verum group. The Tr14 group showed a tendentially lower increase of neutrophils (p=0.10, BDNF (p=0.03, stem cell factor (p=0.09, and GM-CSF (p=0.09 to higher levels. The results of the current study indicate that Tr14 seems to limit exercise-induced muscle damage most likely via attenuation of both innate and adaptive immune responses. This study was registered with ClinicalTrials.gov (NCT01912469.

  12. Análise dos acidentes de trânsito ocorridos em uma Capital do Nordeste Brasileiro, em 2006.

    Directory of Open Access Journals (Sweden)

    Francisco José de Paula Júnior

    2013-06-01

    Full Text Available Este trabalho teve como objetivo descrever os acidentes de trânsito ocorridos na cidade de Fortaleza, capital do Ceará, no ano de 2006. Foi realizado um estudo descritivo, transversal, a partir de dados do Sistema de Informação de Acidentes de Trânsito de Fortaleza, que agrega todas as informações pertinentes aos acidentes de trânsito ocorridos na capital cearense, além de informações do Sistema de Informações Hospitalares. Esses dados foram analisados utilizando o software EPI INFO®. Em 2006, foram registrados 23.443 acidentes de trânsito. Desses, houve uma proporção de 2:1, entre homens e mulheres e os óbitos ocorreram principalmente na faixa etária de 30 a 59 anos, liderados por pedestres 141 (41,34% e motociclistas com 76 (22,28%. Entre os tipos de acidentes 14.267 (60,85% registraram-se colisões, tendo o mês de dezembro e o intervalo de 18 às 20 horas apresentado o maior número de registros. Em relação aos ferimentos, os motociclistas foram os mais lesionados, atingindo 5.634 (40,57% pessoas. Parte significativa dos acidentes e óbitos envolveu adolescentes e adultos jovens, resultando em 341 (0,71% vitimas que evoluíram para óbito. É fundamental estimular políticas públicas para melhoria nas condições de trânsito e um maior investimento para os sistemas que coletam informações sobre os acidentes.

  13. DRIS norms for 'Valencia' sweet orange on three rootstocks Normas DRIS para laranjeira 'Valência' sobre três porta-enxertos

    Directory of Open Access Journals (Sweden)

    Francisco de Assis Alves Mourão Filho

    2003-01-01

    Full Text Available Diagnosis and Recommendation Integrated System (DRIS applies nutrient ratios instead of the isolated concentration values of each nutrient in interpretation of tissue analysis. The objectives of this research were to establish adequate DRIS norms for 'Valencia' sweet orange irrigated commercial groves budded on three rootstocks and correlate indexes of nutrition balance with yield. Experiments were conducted in São Paulo State, Brazil. Rootstocks Rangpur lime, Caipira sweet orange, and Poncirus trifoliata, with more than six years old and yield above 40 ton ha-1 were utilized. Data referred to yield, tree spacing, rootstock and foliar concentrations of N, P, K, Ca, Mg, S, Fe, Mn, Cu, Zn, and B in non fruiting terminals for each grove were processed for the years 1994 through 1998. DRIS indexes were calculated by Nick criterion for choosing the ratio order of the nutrients and Jones calculation method of the ratio functions. Indexes of nutritional balance calculated from DRIS norms presented high correlation with yield for the three scion/rootstock combinations. DRIS norms defined in this research are valid, since leaf sampling is done on non fruiting terminals and the grove is irrigated.O sistema integrado de diagnose e recomendação (DRIS - Diagnosis and Recommendation Integrated System utiliza relações entre nutrientes em vez da concentração absoluta e isolada de cada um deles na interpretação da análise de tecidos. O objetivo deste trabalho foi estabelecer normas para o método DRIS em pomares comerciais irrigados de laranjeira 'Valência' sobre três porta-enxertos e correlacionar os índices de balanço nutricional com a produtividade. Os experimentos foram conduzidos no Estado de São Paulo, com os porta-enxertos limão 'Cravo', laranja 'Caipira' e Poncirus trifoliata, com mais de seis anos, e produtividade acima de 40 t ha-1. Dados de produtividade, espaçamento, porta-enxerto e teores foliares de N, P, K, Ca, Mg, S, Fe, Mn, Cu

  14. Double pion photoproduction off Ca-40

    NARCIS (Netherlands)

    Bloch, F.; Ahrens, J.; Annand, J.R.M.; Beck, R.; Fog, L.S.; Hornidge, D.; Janssen, S.; Kotulla, M.; Krusche, B.; McGeorge, J.C.; MacGregor, I.J.D.; Messchendorp, J.G.; Metag, V.; Novotny, R.; Owens, R.; Pfeiffer, M.; Rost, M.; Sanderson, R.; Schadmand, S.; Thomas, A.; Watts, D.P.

    The pbotoproduction of pi(0)pi(0) and pi(0)pi(+/-) pairs off Ca-40 has been investigated with the TAPS detector using the Glasgow photon tagging spectrometer at the Mainz MAMI accelerator. Data have been taken for incident photon energies in the energy range from 200-820MeV. Total cross-sections

  15. Numerical model of Ca(OH)

    NARCIS (Netherlands)

    Koster, T.; Peelen, W.; Larbi, J.; Rooij, M. de; Polder, R.

    2010-01-01

    A mathematical model is being developed to describe a repair method in concrete, called cathodic protection (CP). The model is in principle also useful to describe electrodeposition in concrete, e.g. the process of re-precipitation of Ca(OH)2 invoked by an electrical current. In CP, the

  16. Hippocampal CA1 Ripples as Inhibitory Transients.

    Directory of Open Access Journals (Sweden)

    Paola Malerba

    2016-04-01

    Full Text Available Memories are stored and consolidated as a result of a dialogue between the hippocampus and cortex during sleep. Neurons active during behavior reactivate in both structures during sleep, in conjunction with characteristic brain oscillations that may form the neural substrate of memory consolidation. In the hippocampus, replay occurs within sharp wave-ripples: short bouts of high-frequency activity in area CA1 caused by excitatory activation from area CA3. In this work, we develop a computational model of ripple generation, motivated by in vivo rat data showing that ripples have a broad frequency distribution, exponential inter-arrival times and yet highly non-variable durations. Our study predicts that ripples are not persistent oscillations but result from a transient network behavior, induced by input from CA3, in which the high frequency synchronous firing of perisomatic interneurons does not depend on the time scale of synaptic inhibition. We found that noise-induced loss of synchrony among CA1 interneurons dynamically constrains individual ripple duration. Our study proposes a novel mechanism of hippocampal ripple generation consistent with a broad range of experimental data, and highlights the role of noise in regulating the duration of input-driven oscillatory spiking in an inhibitory network.

  17. literacy.ca EXPRESS. April 2010

    Science.gov (United States)

    Movement for Canadian Literacy, 2010

    2010-01-01

    This issue of "literacy.ca EXPRESS" focuses on poverty. The articles included in this issue are: (1) Poverty Overview; (2) Tony's Story; (3) LAN (Learner Advisory Network) Member's Story (Dianne Smith); (4) Linking Adult Literacy to Poverty Reduction; (5) MCL (Movement for Canadian Literacy) Update; (6) Highlights from the LAN; (7) Good…

  18. Ca2+ signalling in the myocardium by (redox regulation of PKA/CaMKII

    Directory of Open Access Journals (Sweden)

    Alex S Johnston

    2015-08-01

    Full Text Available Homeostatic cardiac function is maintained by a complex network of interdependent signaling pathways which become compromised during disease progression. Excitation-contraction-coupling, the translation of an electrical signal to a contractile response is critically dependent on a tightly controlled sequence of events culminating in a rise in intracellular Ca2+ and subsequent contraction of the myocardium. Dysregulation of this Ca2+ handling system as well as increases in the production of reactive oxygen species (ROS are two major contributing factors to myocardial disease progression. ROS, generated by cellular oxidases and by-products of cellular metabolism, are highly reactive oxygen derivatives that function as key secondary messengers within the heart and contribute to normal homeostatic function. However, excessive production of ROS, as in disease, can directly interact with kinases critical for Ca2+ regulation. This post-translational oxidative modification therefore links changes in the redox status of the myocardium to phospho-regulated pathways essential for its function. This review aims to describe the oxidative regulation of the Ca2+/calmodulin-dependent kinase II (CaMKII and cAMP-dependent protein kinase A (PKA, and the subsequent impact this has on Ca2+ handling within the myocardium. Elucidating the impact of alterations in intracellular ROS production on Ca2+ dynamics through oxidative modification of key ROS sensing kinases, may provide novel therapeutic targets for preventing myocardial disease progression.

  19. Ca2+ ionophores trigger membrane remodeling without a need for store-operated Ca2+ entry.

    Science.gov (United States)

    Galitzine, Marie; Capiod, Thierry; Le Deist, Françoise; Meyer, Dominique; Freyssinet, Jean-Marie; Kerbiriou-Nabias, Danièle

    2005-02-04

    Calcium (Ca2+) ionophores are the most effective agents able to elicit rapid membrane remodeling in vitro. This process exposes aminophospholipids at the surface of platelets and blood cells, thus providing a catalytic surface for coagulation. To explore the underlying mechanism, we examined if cytosolic Ca2+ ([Ca2+]i) increase through store-operated Ca2+ entry (SOCE) was necessary for the potent effect of ionophores. Recent studies have demonstrated that the Ca2+-ATPase inhibitor thapsigargin, although able to elevate [Ca2+]i through SOCE, does not trigger the rapid membrane remodeling. However, it was not known if the additional effect of ionophores to promote the process required SOCE or could it occur independently. We took advantage of two mutant B lymphoblast cell lines, characterized either by defective SOCE or altered membrane remodeling, to simultaneously assess [Ca2+]i increase and membrane remodeling in the presence of ionophores or thapsigargin. Results imply that ionophores trigger membrane remodeling without the requirement for a functional SOCE.

  20. Mg-Ca Alloys Produced by Reduction of CaO: Understanding of ECO-Mg Alloy Production

    Science.gov (United States)

    Jung, In-Ho; Lee, Jin Kyu; Kim, Shae K.

    2017-04-01

    There have been long debates about the environment conscious (ECO) Mg technology which utilizes CaO to produce Ca-containing Mg alloys. Two key process technologies of the ECO-Mg process are the chemical reduction of CaO by liquid Mg and the maintenance of melt cleanliness during the alloying of Ca. Thermodynamic calculations using FactSage software were performed to explain these two key issues. In addition, an experimental study was performed to compare the melt cleanliness of the Ca-containing Mg alloys produced by the conventional route with metallic Ca and the ECO-Mg route with CaO.

  1. Ultrastructural and Immunohistochemical Localization of the Plasma Membrane Ca2+-ATPase 4 (PMCA4) in Ca2+-Transporting Epithelia

    DEFF Research Database (Denmark)

    Alexander, R Todd; Beggs, Megan R; Zamani, Reza

    2015-01-01

    Plasma Membrane Ca(2+)-ATPase's (PMCA) participate in epithelial Ca(2+) transport and intracellular Ca(2+) signaling. The Pmca4 isoform is enriched in distal nephron isolates and decreased in mice lacking the epithelial Ca(2+) channel, Trpv5. We therefore hypothesized that Pmca4 plays a significa...... to a housekeeping function of the pump in Ca(2+) transporting epithelia. In conclusion, Pmca4 shows a divergent expression pattern in Ca(2+) transporting epithelia, inferring diverse roles for this isoform not limited to transepithelial Ca(2+) transport....

  2. Genome Sequencing and Comparative Analysis of the Biocontrol Agent Trichoderma harzianum sensu stricto TR274

    Energy Technology Data Exchange (ETDEWEB)

    Steindorff, Andrei S.; Noronha, Elilane F.; Ulhoa, Cirano J.; Kuo, Alan; Salamov, Asaf A.; Haridas, Sajeet; Riley, Robert W.; Druzhinina, Irina S.; Kubicek, Christian P.; Grigoriev, Igor V.

    2015-03-17

    Biological control is a complex process which requires many mechanisms and a high diversity of biochemical pathways. The species of Trichoderma harzianum are well known for their biocontrol activity against many plant pathogens. To gain new insights into the biocontrol mechanism used by T. harzianum, we sequenced the isolate TR274 genome using Illumina. The assembly was performed using AllPaths-LG with a maximum coverage of 100x. The assembly resulted in 2282 contigs with a N50 of 37033bp. The genome size generated was 40.8 Mb and the GC content was 47.7%, similar to other Trichoderma genomes. Using the JGI Annotation Pipeline we predicted 13,932 genes with a high transcriptome support. CEGMA tests suggested 100% genome completeness and 97.9% of RNA-SEQ reads were mapped to the genome. The phylogenetic comparison using orthologous proteins with all Trichoderma genomes sequenced at JGI, corroborates the Trichoderma (T. asperellum and T. atroviride), Longibrachiatum (T. reesei and T. longibrachiatum) and Pachibasium (T. harzianum and T. virens) section division described previously. The comparison between two Trichoderma harzianum species suggests a high genome similarity but some strain-specific expansions. Analyses of the secondary metabolites, CAZymes, transporters, proteases, transcription factors were performed. The Pachybasium section expanded virtually all categories analyzed compared with the other sections, specially Longibrachiatum section, that shows a clear contraction. These results suggests that these proteins families have an important role in their respective phenotypes. Future analysis will improve the understanding of this complex genus and give some insights about its lifestyle and the interactions with the environment.

  3. Resultados del tratamiento a pacientes con tríada terrible del codo

    Directory of Open Access Journals (Sweden)

    Héctor Gabriel Díaz Carrillo

    2015-04-01

    Full Text Available La luxación del codo, asociada a la fractura del proceso coronoideo y a la fractura de la cabeza radial, es denominada “tríada terrible del codo”, debido a la complejidad al abordar el tratamiento correcto y lo desalentador de los resultados que se obtienen con el mismo. Se realizó un estudio prospectivo, donde se recogen los resultados postoperatorios, obtenidos en cuatro pacientes con inestabilidad traumática aguda del codo, tratados entre enero de 2012 y mayo de 2013, que acudieron inicialmente al cuerpo de guardia del Hospital General Docente “Dr. Ernesto Guevara de La Serna” y tuvieron un seguimiento medio de ocho meses. El proceder consistió en reparación de la coronoides, del ligamento lateral externo, reparación de la cúpula radial, cupulectomía y colocación de pin endomedular; la técnica operatoria a emplear se definió por los estudios radiográficos realizados y los hallazgos en el transcurso del proceder. Los pacientes, tres hombres y una mujer, tuvieron cinco codos lesionados y un promedio de edad de 34 años. Como resultado del tratamiento la flexión fue normal entre 130-135° en 4 codos y limitada en uno a 110°, la extensión se encontró limitada de 8 a 21° en todos los pacientes, la pronosupinación se mantuvo limitada en todos los casos entre 5 y 22°. La intervención quirúrgica mediante fijación y reparación de los estabilizadores del codo proporcionó a corto plazo una funcionabilidad del codo cercana a la normalidad, los pacientes evolucionaron positivamente. Las complicaciones fueron: reluxación, infección local y aflojamiento de la osteosíntesis

  4. Klatring i trær og hyttebygging: - om barns lek og lekesteder ute

    Directory of Open Access Journals (Sweden)

    Merete Lund Fasting

    2015-07-01

    Full Text Available Abstract: This article describes 10 year old children`s play in nature during school hours. However, my result does equally reflect on children in kindergarden as well. Observation and conversation was used as methods as well as pictures. The children’s play in the trees and building huts was conducted in groups, and their communication was nonverbal with each other and with the nature. However; the girls reveal that they enjoy their conversations while being in the tree. The children often play at the same place over and over. This is significant to the evolution of the play and the children’s communication with their places in nature.Sammendrag: Denne artikkelen omhandler 10 år gamle barns lek på natursteder i skoletida. Selv om studien omhandler 10 åringer så kan kunnskapen den frembringer også være viktig for å forstå barnehagebarns lek og tilknytning til sine lekesteder i barnehagen. Metodene som ble brukt for å undersøke barnas lek var observasjoner og samtaler med barna, i tillegg til fotografier. Barnas lek i trærne og i hyttebyggingen karakteriseres ved at de er flere sammen, og barna har en kroppslig dialog med hverandre og omgivelsene i leken. Barna snakker i liten grad sammen om leken og hvordan de skal leke; deres kommunikasjon er hovedsakelig nonverbal. Men når jentene sitter i treet forteller de meg at de liker å sitte og snakke. Barna leker på steder hvor de har vært mye før, og dette har en betydning for barnas lek og barnas kommunikasjon med deres steder.

  5. Vulnerabilidade humana no contexto do trânsito motociclístico

    Directory of Open Access Journals (Sweden)

    Marcelo Moreira Corgozinho

    Full Text Available Resumo O aspecto fundamental da vulnerabilidade enquanto princípio ético da Declaração Universal sobre Bioética e Direitos Humanos é o de formular uma obrigação de ação moral diante das situações que fragilizam determinados grupos da sociedade. Nesse sentido, este estudo objetiva promover uma reflexão, a partir do princípio do respeito pela vulnerabilidade humana, utilizando como público-alvo motociclistas e sua experiência com o uso da moto. Trata-se de estudo com abordagem qualitativa, utilizando metodologia quantiqualitativa para análise das entrevistas dos participantes. A partir da experiência dos motociclistas com a utilização da motocicleta foi possível apresentar alguns apontamentos que vão ao encontro da necessidade de respeito pela vulnerabilidade humana, a saber: potencialização da vulnerabilidade intrínseca ao ser humano; vulnerabilidade social; e responsabilidade social do Estado diante da utilização da motocicleta. Ao considerar o respeito pela vulnerabilidade humana e a utilização da motocicleta, torna-se necessário correlacionar aspectos que envolvam a responsabilidade social com situações de suscetibilidade humana ao agravo. Assim, torna-se fundamental a implementação de políticas de intervenção que abarquem as dimensões econômica, cultural e política, por meio da intersetorialidade, para que se consiga alcançar efetivamente todos os atores envolvidos na dinâmica da segurança do trânsito motociclístico: Estado, autoridades de fiscalização, fabricantes de veículos, condutores, pedestres e ciclistas.

  6. Concordance of DSM-5 and DSM-IV-TR classifications for autism spectrum disorder.

    Science.gov (United States)

    Ohashi, Kei; Mizuno, Yoshifumi; Miyachi, Taishi; Asai, Tomoko; Imaeda, Masayuki; Saitoh, Shinji

    2015-12-01

    The fifth edition of the Diagnostic and Statistical Manual of Mental Disorders (DSM-5) was published in May 2013. Autism spectrum disorder (ASD) has been structured for the three subtypes of pervasive developmental disorder (PDD), but the number of impairment in social and communication dimension is not stated. The subjects were 68 children who visited the Department of Psychology and Development at Nagoya City University Hospital for the first time between the ages of 6 and 15 years old. We retrospectively re-examined the subjects using DSM-IV-TR criteria and DSM-5 criteria with two rules (two of three and one of three on the social and communication dimension) and examined the concordance rate. Forty subjects were diagnosed with PDD, and 28 were not. The mean PDD subject age was 9.4 years, and mean IQ was 84.0 on the Wechsler Intelligence Scale for Children III or 62.7 on the Tanaka-Binet test. Twenty-seven (68%) of the PDD subjects were classified with ASD using DSM-5 criteria when the two of three rule was applied, while 32 (80%) were classified with ASD when the one of three rule was applied. All subjects without PDD were not diagnosed with ASD on DSM-5 criteria. DSM-5 criteria may exclude high functioning and older subjects from ASD because they tend to be atypical. The diagnostic procedure for DSM-5 criteria is ambiguous, especially in high functioning subjects and those diagnosed at an older age. © 2015 Japan Pediatric Society.

  7. Exílio: uma história em três dimensões

    Directory of Open Access Journals (Sweden)

    Heloisa Paulo

    2014-06-01

    Full Text Available No século XX, a ascensão dos regimes fascistas leva ao exílio opositores que procuravam fugir da morte pela execução sumária ou pelo confinamento aos campos de concentração. Portugal conhece um dos mais longos regimes de força, levando seus opositores a um longo degredo que, para uma grande maioria, durou toda a vida. Estes exilados e emigrados políticos buscaram refúgio nas mais diferentes partes do mundo, quer nos antigos núcleos de emigração ou em países onde o Estado democrático favorecia seu acolhimento. E se as histórias de vida exigem a recuperação de diversos "espaços históricos", no caso dos exilados estes "espaços" possuem uma outra dimensão. À dimensão local, origem e formação dos personagens e ao contexto do "nacional", em que estão inseridos num momento de maioridade, podemos acrescentar outro, "supranacional", ou seja, a história dos países de acolhimento, uma base para a compreensão das relações estabelecidas no exílio. Tomando como ponto de partida o caso português, este trabalho pretende ressaltar alguns pontos importantes para a elaboração de biografias de exilados, tendo-se em conta a necessidade de cruzamento dos três espaços citados, a releitura da historiografia já produzida e os problemas da "memória oficial".

  8. Comportamento no trânsito entre estudantes de medicina Traffic behavior of medical students

    Directory of Open Access Journals (Sweden)

    Daniel Colicchio

    2010-01-01

    Full Text Available OBJETIVOS: Estudar o comportamento de estudantes de medicina em relação ao trânsito, comparando os padrões atuais com aqueles observados em estudo realizado anteriormente na mesma instituição. MÉTODOS: Foram aplicados questionários padronizados em uma amostra de estudantes regularmente matriculados no curso médico da Faculdade de Medicina de Ribeirão Preto, objetivando coletar informações demográficas, socioeconômicas e referentes ao comportamento no trânsito e envolvimento em acidentes. Esses resultados foram comparados com os obtidos em investigação semelhante, realizada em 1997. RESULTADOS: Foram estudados 354 alunos em 2008 e 377 em 1997. Do total, 431 (59% já haviam dirigido logo após a ingestão de álcool, sendo que, desses, 21,5% em condições de intensa alcoolização. Participação em rachas foi referida por 10,5% dos participantes, com diferença marcante entre os sexos (12,5% entre homens e 3,4% em mulheres. Envolvimento em acidentes com vítimas, fatais ou não, foi referido por 19,2% dos participantes. Esses percentuais atingiram 31,1% entre os que já dirigiram intensamente alcoolizados e 42,9% entre os que participaram de rachas. O estudo de 2008 evidenciou maior uso de capacete e de cinto de segurança em meio urbano e redução de uso de bebidas alcoólicas antes de dirigir. Por outro lado, mostrou redução de respeito contínuo à sinalização de semáforos. CONCLUSÃO: Comportamentos de risco no trânsito fazem-se presentes com elevada frequência entre os participantes, estando diretamente relacionados com o envolvimento em acidentes que provocam vítimas. Visto ser essa uma parcela populacional diferenciada, com conhecimento e contato direto com as consequências desses acidentes, esperar-se-ia uma menor presença de comportamentos de elevado risco. As informações oriundas desse trabalho devem conduzir a uma reflexão por parte da comunidade acadêmica com vistas à introdução de programas

  9. Reassigning the CaH+ 11Σ → 21Σ vibronic transition with CaD+

    Science.gov (United States)

    Condoluci, J.; Janardan, S.; Calvin, A. T.; Rugango, R.; Shu, G.; Sherrill, C. D.; Brown, K. R.

    2017-12-01

    We observe vibronic transitions in CaD+ between the 11Σ and 21Σ electronic states by resonance enhanced multiphoton photodissociation spectroscopy in a Coulomb crystal. The vibronic transitions are compared with previous measurements on CaH+. The result is a revised assignment of the CaH+ vibronic levels and a disagreement with multi-state-complete-active-space second-order perturbation theory theoretical calculations by approximately 700 cm-1. Updated high-level coupled-cluster calculations that include core-valence correlations reduce the disagreement between theory and experiment to 300 cm-1.

  10. Serum concentrations of the biomarkers CA125, CA15-3, CA72-4, tPSA and PAPP-A in natural and stimulated ovarian cycles

    Directory of Open Access Journals (Sweden)

    Melissa Stemp

    2014-06-01

    Conclusion: Ovarian stimulation reduced serum PAPP‐A levels, CA125 and CA15‐3 levels were generally unaffected by ovarian stimulation but displayed cyclical changes throughout both natural and stimulated cycles, whilst tPSA and CA72-4 were not affected by the stage of the cycle or ovarian stimulation.

  11. Tržišta rada za imigrante: usporedba integracije na tržištima rada imigranata iz Turske i bivše Jugoslavije u Njemačkoj i Austriji

    OpenAIRE

    Fassmann, Heinz; Münz, Rainer; Seifert, Wolfgang

    1999-01-01

    U radu se uspoređuju radna mjesta koja zauzimaju radnici iz Turske i bivše Jugoslavije i njihova djeca u Njemačkoj i Austriji. U obje zemlje gostovanja te dvije imigrantske skupine sačinjavaju glavninu strane radne snage. Analize pokazuju da su one u mnogo većoj mjeri koncentrirane na manje važnim (sporednim) položajima u hijerarhiji tržišta rada u Austriji nego u Njemačkoj. Ta se činjenica ne mijenja ni kada se u obzir uzmu školovanje i duljina boravka. Struktura tržišta rada Njemačke, u usp...

  12. The potential of chitosan in enhancing peptide and protein absorption across the TR146 cell culture model-an in vitro model of the buccal epithelium

    DEFF Research Database (Denmark)

    Portero, Ana; Remuñán-López, Carmen; Nielsen, Hanne Mørck

    2002-01-01

    To investigate the potential of chitosan (CS) to enhance buccal peptide and protein absorption, the TR146 cell culture model, a model of the buccal epithelium, was used.......To investigate the potential of chitosan (CS) to enhance buccal peptide and protein absorption, the TR146 cell culture model, a model of the buccal epithelium, was used....

  13. Investigation of a solar heating system for space heating and domestic hot water supply for Sol&Træ A.m.b.a

    DEFF Research Database (Denmark)

    Vejen, Niels Kristian

    1999-01-01

    A solar heating system for space heating and domestic hot water supply from "Sol&Træ A.m.b.a." was tested in a laboratory test facility.......A solar heating system for space heating and domestic hot water supply from "Sol&Træ A.m.b.a." was tested in a laboratory test facility....

  14. Chimeric bispecific OC/TR monoclonal antibody mediates lysis of tumor cells expressing the folate-binding protein (MOv18) and displays decreased immunogenicity in patients

    NARCIS (Netherlands)

    Luiten, R. M.; Warnaar, S. O.; Sanborn, D.; Lamers, C. H.; Bolhuis, R. L.; Litvinov, S. V.; Zurawski, V. R.; Coney, L. R.

    1997-01-01

    The bispecific OC/TR monoclonal antibody (mAb) cross-links the CD3 molecule on T cells with the human folate-binding protein (FBP), which is highly expressed on nonmucinous ovarian carcinomas. Clinical trials of patients with ovarian carcinoma with the OC/TR mAb have shown some complete and partial

  15. Uniprindi büroo- ja trükikojahoone : edu peatänavatest, Uniprindi trükikojast ning geneerilisest arhitektuurist = Success from Main Streets, Uniprint Printing Plant, and Generic Architecture / Veljo Kaasik

    Index Scriptorium Estoniae

    Kaasik, Veljo

    2000-01-01

    Pärnu mnt. ristmiku juurde Tallinnas R. Puusepa arhitektibüroo poolt trükikojale Uniprint projekteeritud büroo-tootmishoonest. Arhitektid Raivo Puusepp, Krista Väärtnõu. Konstruktsioonid: Projektbüroo Teinos, Civen OÜ. Projekt 1999-2000, hoone valmis 2000. Peatänavaid ääristava hoonestuse kiirest muundumisest. 9 ill.: korruste plaanid, lõiked, vaated (joon.); välisvaated (foto)

  16. Precise half-life measurements for $^{38}$Ca and $^{39}$Ca

    CERN Document Server

    Blank, B; Demonchy, C-E; Borge, M J G; Matea, I; Munoz, F; Huikari, J; Dominguez-Reyes, R; Plaisir, C; Sturm, S; Canchel, G; Delahaye, P; Audirac, L; Fraile, L M; Serani, L; Lunney, D; Pedroza, J-L; Bey, A; Souin, J; Hui, Tran Trong; Delalee, F; Tengblad, O; Wenander, F

    2010-01-01

    The half-lives of Ca-38 and Ca-39 have been measured at ISOLDE of CERN. The REXTRAP facility was used to prepare ultra-clean samples of radioactive nuclei for precision decay spectroscopy. Ca-38 is one of the T-z = -1, 0(+). 0(+) beta-emitting nuclides used to determine the vector coupling constant of the weak interaction and the V-ud quark-mixing matrix element. The result obtained, T-1/2 = 443.8(19) ms, is four times more precise than the average of previous measurements. For Ca-39, a half-life of T-1/2 = 860.7(10) ms is obtained, a result in agreement with the average value from the literature.

  17. Precise half-life measurements for {sup 38}Ca and {sup 39}Ca

    Energy Technology Data Exchange (ETDEWEB)

    Blank, B.; Bey, A.; Matea, I.; Souin, J.; Audirac, L.; Canchel, G.; Delalee, F.; Demonchy, C.E.; Giovinazzo, J.; Trong Hui, Tran; Huikari, J.; Munoz, F.; Pedroza, J.L.; Plaisir, C.; Serani, L. [Universite Bordeaux 1 - UMR 5797 CNRS/IN2P3, Centre d' etudes nucleaires de Bordeaux Gradignan, Gradignan (France); Borge, M.J.G.; Dominguez-Reyes, R.; Tengblad, O. [CSIC, Instituto de Estructura de la Materia, Madrid (Spain); Delahaye, P.; Wenander, F. [CERN, Geneva (Switzerland); Fraile, L.M. [Universidad Complutense, Grupo de Fisica Nuclear, Facultad CC. Fisicas, Madrid (Spain); Lunney, D. [Universite de Paris Sud, Centre de Spectrometrie Nucleaire et de Spectrometrie de Masse, (CNRS/IN2P3), Orsay (France); Sturm, S. [Johannes Gutenberg University, Department of Physics, Mainz (Germany)

    2010-06-15

    The half-lives of {sup 38}Ca and {sup 39}Ca have been measured at ISOLDE of CERN. The REXTRAP facility was used to prepare ultra-clean samples of radioactive nuclei for precision decay spectroscopy. {sup 38}Ca is one of the T{sub z}=-1,0{sup +} {yields}0{sup +}{beta} -emitting nuclides used to determine the vector coupling constant of the weak interaction and the V{sub ud} quark-mixing matrix element. The result obtained, T{sub 1/2}=443.8(19) ms, is four times more precise than the average of previous measurements. For {sup 39}Ca, a half-life of T{sub 1/2}=860.7(10) ms is obtained, a result in agreement with the average value from the literature. (orig.)

  18. Generation and behavior characterization of CaMKIIβ knockout mice.

    Directory of Open Access Journals (Sweden)

    Adam D Bachstetter

    Full Text Available The calcium/calmodulin-dependent protein kinase II (CaMKII is abundant in the brain, where it makes important contributions to synaptic organization and homeostasis, including playing an essential role in synaptic plasticity and memory. Four genes encode isoforms of CaMKII (α, β, δ, γ, with CaMKIIα and CaMKIIβ highly expressed in the brain. Decades of molecular and cellular research, as well as the use of a large number of CaMKIIα mutant mouse lines, have provided insight into the pivotal roles of CaMKIIα in brain plasticity and cognition. However, less is known about the CaMKIIβ isoform. We report the development and extensive behavioral and phenotypic characterization of a CaMKIIβ knockout (KO mouse. The CaMKIIβ KO mouse was found to be smaller at weaning, with an altered body mass composition. The CaMKIIβ KO mouse showed ataxia, impaired forelimb grip strength, and deficits in the rotorod, balance beam and running wheel tasks. Interestingly, the CaMKIIβ KO mouse exhibited reduced anxiety in the elevated plus maze and open field tests. The CaMKIIβ KO mouse also showed cognitive impairment in the novel object recognition task. Our results provide a comprehensive behavioral characterization of mice deficient in the β isoform of CaMKII. The neurologic phenotypes and the construction of the genotype suggest the utility of this KO mouse strain for future studies of CaMKIIβ in brain structure, function and development.

  19. Characterization of Binding Epitopes of CA125 Monoclonal Antibodies

    DEFF Research Database (Denmark)

    Marcos-Silva, Lara; Narimatsu, Yoshiki; Halim, Adnan

    2014-01-01

    analysis of N- and O-glycosylation sites of a MUC16 TR expressed in CHO cells and demonstrate that neither N- nor O-glycosylation appear to substantially influence binding of OC125 and M11 mAbs. A series of successive N- and C-terminal truncations of a MUC16 TR construct expressed in E. coli narrowed down...... constructs with and without aberrant glycosylation now opens the way for vaccine studies....

  20. Glutamate receptor activation in cultured cerebellar granule cells increases cytosolic free Ca2+ by mobilization of cellular Ca2+ and activation of Ca2+ influx

    DEFF Research Database (Denmark)

    Bouchelouche, P; Belhage, B; Frandsen, A

    1989-01-01

    The Ca2+ sensitive fluorescent probe, fura-2 has been used to monitor cytosolic free calcium levels in mature primary cultures of cerebellar granule cells during exposure to L-glutamate and other excitatory amino acids: quisqualate (QA) kainate (KA) and N-methyl-D-aspartate (NMDA). Glutamate...... at micromolar concentrations produced a prompt and dose-related increase in the intracellular concentration of free Ca2+, ([Ca2+]i), whereas QA, KA and NMDA had no effect. This increase was also seen in the absence of extracellular Ca2+, suggesting that L-glutamate promotes mobilization of Ca2+ from...

  1. Ca2+-binding protein-1 facilitates and forms a postsynaptic complex with Cav1.2 (L-type) Ca2+ channels.

    Science.gov (United States)

    Zhou, Hong; Kim, Seong-Ah; Kirk, Elizabeth A; Tippens, Alyssa L; Sun, Hong; Haeseleer, Françoise; Lee, Amy

    2004-05-12

    Ca2+-binding protein-1 (CaBP1) is a Ca2+-binding protein that is closely related to calmodulin (CaM) and localized in somatodendritic regions of principal neurons throughout the brain, but how CaBP1 participates in postsynaptic Ca2+ signaling is not known. Here, we describe a novel role for CaBP1 in the regulation of Ca2+ influx through Ca(v)1.2 (L-type) Ca2+ channels. CaBP1 interacts directly with the alpha1 subunit of Ca(v)1.2 at sites that also bind CaM. CaBP1 binding to one of these sites, the IQ domain, is Ca2+ dependent and competitive with CaM binding. The physiological significance of this interaction is supported by the association of Ca(v)1.2 and CaBP1 in postsynaptic density fractions purified from rat brain. Moreover, in double-label immunofluorescence experiments, CaBP1 and Ca(v)1.2 colocalize in numerous cell bodies and dendrites of neurons, particularly in pyramidal cells in the CA3 region of the hippocampus and in the dorsal cortex. In electrophysiological recordings of cells transfected with Ca(v)1.2, CaBP1 greatly prolonged Ca2+ currents, prevented Ca2+-dependent inactivation, and caused Ca2+-dependent facilitation of currents evoked by step depolarizations and repetitive stimuli. These effects contrast with those of CaM, which promoted strong Ca2+-dependent inactivation of Ca(v)1.2 with these same voltage protocols. Our findings reveal how Ca2+-binding proteins, such as CaM and CaBP1, differentially adjust Ca2+ influx through Ca(v)1.2 channels, which may specify diverse modes of Ca2+ signaling in neurons.

  2. Ca2+/calmodulin-dependent protein kinase II-dependent remodeling of Ca2+ current in pressure overload heart failure.

    Science.gov (United States)

    Wang, Yanggan; Tandan, Samvit; Cheng, Jun; Yang, Chunmei; Nguyen, Lan; Sugianto, Jessica; Johnstone, Janet L; Sun, Yuyang; Hill, Joseph A

    2008-09-12

    Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) activity is increased in heart failure (HF), a syndrome characterized by markedly increased risk of arrhythmia. Activation of CaMKII increases peak L-type Ca(2+) current (I(Ca)) and slows I(Ca) inactivation. Whether these events are linked mechanistically is unknown. I(Ca) was recorded in acutely dissociated subepicardial and subendocardial murine left ventricular (LV) myocytes using the whole cell patch clamp method. Pressure overload heart failure was induced by surgical constriction of the thoracic aorta. I(Ca) density was significantly larger in subepicardial myocytes than in subendocardial/myocytes. Similar patterns were observed in the cell surface expression of alpha1c, the channel pore-forming subunit. In failing LV, I(Ca) density was increased proportionately in both cell types, and the time course of I(Ca) inactivation was slowed. This typical pattern of changes suggested a role of CaMKII. Consistent with this, measurements of CaMKII activity revealed a 2-3-fold increase (p process could not be induced, suggesting already maximal activation. Internal application of active CaMKII in failing myocytes did not elicit changes in I(Ca). Finally, CaMKII inhibition by internal diffusion of a specific peptide inhibitor reduced I(Ca) density and inactivation time course to similar levels in control and HF myocytes. I(Ca) density manifests a significant transmural gradient, and this gradient is preserved in heart failure. Activation of CaMKII, a known pro-arrhythmic molecule, is a major contributor to I(Ca) remodeling in load-induced heart failure.

  3. Role of Ca++, CaATPase and alkaline protease in cardiac contraction band formation.

    Science.gov (United States)

    Smith, A N; Marquet, E; Sobel, H J

    1982-07-01

    Cardiac cells' total homogenates and subcellular components Ca++, CaATPase and alkaline protease levels were matched with their respective microscopic samples in order to ascertain their role in the formation of contraction bands. It was seen that those samples having contraction bands also had a high incidence of intracellular Ca++, low CaATPase and high alkaline protease levels. This study confirms reports that contraction bands are not limited to pathological hearts (Adomian et al., 1977), but neither are they limited to artifacts related to autopsy procedures. They were seen in varying degrees of severity in hearts of healthy rats sacrificed by decapitation and less frequently with only sarcomeric involvement to absence of in animals sacrificed by diethyl ether overdose. It is postulated that the elevated Ca++ activated alkaline protease (pH 8.5) by inhibiting its inhibitor (Waxman and Krebs, 1978). It may have inhibited total CaATPase activity (Hasselbach, 1974) as well. We believe that the activated alkaline protease produced irreversible contraction bands by lysing the microhinge between light and heavy meromyosin.

  4. Ca-α1T, a fly T-type Ca2+ channel, negatively modulates sleep.

    Science.gov (United States)

    Jeong, Kyunghwa; Lee, Soyoung; Seo, Haengsoo; Oh, Yangkyun; Jang, Donghoon; Choe, Joonho; Kim, Daesoo; Lee, Jung-Ha; Jones, Walton D

    2015-12-09

    Mammalian T-type Ca(2+) channels are encoded by three separate genes (Cav3.1, 3.2, 3.3). These channels are reported to be sleep stabilizers important in the generation of the delta rhythms of deep sleep, but controversy remains. The identification of precise physiological functions for the T-type channels has been hindered, at least in part, by the potential for compensation between the products of these three genes and a lack of specific pharmacological inhibitors. Invertebrates have only one T-type channel gene, but its functions are even less well-studied. We cloned Ca-α1T, the only Cav3 channel gene in Drosophila melanogaster, expressed it in Xenopus oocytes and HEK-293 cells, and confirmed it passes typical T-type currents. Voltage-clamp analysis revealed the biophysical properties of Ca-α1T show mixed similarity, sometimes falling closer to Cav3.1, sometimes to Cav3.2, and sometimes to Cav3.3. We found Ca-α1T is broadly expressed across the adult fly brain in a pattern vaguely reminiscent of mammalian T-type channels. In addition, flies lacking Ca-α1T show an abnormal increase in sleep duration most pronounced during subjective day under continuous dark conditions despite normal oscillations of the circadian clock. Thus, our study suggests invertebrate T-type Ca(2+) channels promote wakefulness rather than stabilizing sleep.

  5. Anode process on platinum in CaCl2-CaO-based melt

    Directory of Open Access Journals (Sweden)

    K. V. Tatarenko

    2014-12-01

    Full Text Available Methods potentiostatic polarization and cyclic voltammetry obtained new data on the mechanism and kinetics of anodic processes on platinum in the molten CaCl2-KCl-CaO when 725–775 °C. Given thermodynamic values of potential difference probable total reactions in the range of the studied temperature. Using potentiostatic polarization and cyclic voltammetry obtained new data on the mechanism and kinetics of anodic process on platinum in the molten CaCl2-KCl-CaO when 725–775 °C thermodynamic assessment of the probability of occurrence total reactions during electrolysis melt on the basis of CaCl2-CaO using non-carbon anode. It is shown that at high current densities anodic process takes place mainly in the conditions of slow diffusion of electrically active particles to the anode, and at low densities (up to 10 mA/cm2 to their discharge to the atomic and lecular oxygen is preceded by a stage, which can be associated with adsorption of atoms of oxygen or with the formation of an oxide film on the surface of platinum. To detect the nature of this stage, further research is needed.

  6. Somatic PIK3CA mutations in seven patients with PIK3CA-related overgrowth spectrum.

    Science.gov (United States)

    Yeung, Kit San; Ip, Janice Jing Kun; Chow, Chin Pang; Kuong, Evelyn Yue Ling; Tam, Paul Kwong-Hang; Chan, Godfrey Chi-Fung; Chung, Brian Hon-Yin

    2017-04-01

    Somatic mutations in PIK3CA cause many overgrowth syndromes that have been recently coined the "PIK3CA-Related Overgrowth Spectrum." Here, we present seven molecularly confirmed patients with PIK3CA-Related Overgrowth Spectrum, including patients with Congenital Lipomatous Overgrowth, Vascular Malformations, Epidermal Nevi, Scoliosis/Skeletal and Spinal syndrome, Klippel-Trenaunay syndrome, lymphatic malformation and two with atypical phenotypes that cannot be classified into existing disease categories. The literature on PIK3CA-Related Overgrowth Spectrum, suggests that PIK3CA c.1258T>C; p.(Cys420Arg), c.1624G>A; p.(Glu542Lys), c.1633G>A; p.(Glu545Lys), c.3140A>G; p.(His1047Arg), and c.3140A>T; p.(His1047Leu) can be identified in approximately 90% of patients without brain overgrowth. Therefore, droplet digital polymerase chain reaction targeting these mutation hotspots could be used as the first-tier genetic test on patients with PIK3CA-Related Overgrowth Spectrum who do not have signs of overgrowth in their central nervous system. © 2017 Wiley Periodicals, Inc. © 2017 Wiley Periodicals, Inc.

  7. "Jag har typ en tallinje i huvudet" : Elevers upplevelse av träning med applikationen Vektor

    OpenAIRE

    Åbonde, Lena; Strömbom, Carina

    2017-01-01

    Syftet med denna studie var att undersöka hur elever i behov av särskilt stöd i matematik beskriver sin upplevelse av träning med applikationen Vektor och om de uttrycker någon förändring av sina grundläggande strategier efter träning. Flera forskare är överens om att arbeta med hjälp av digitala verktyg får effekter på elever både när det gäller kunskap och motivation. Vad det är som gör att de blir motiverade och lär sig mer och om effekterna sitter i, finns det delade meningar om. Åtta ele...

  8. Poblaciones-Mercancía: Tráfico y trata de mujeres en España

    OpenAIRE

    García Cuesta, Sara; López Sala, Ana María; Hernández Corrochano, Elena; Mena Martínez, Luis

    2011-01-01

    Se trata de la primera publicación que hace la Delegación del Gobierno para la Violencia de Género a fin de ahondar en el fenómeno de la Trata de Seres Humanos con fines de explotación sexual. El estudio refleja la situación en España de las mujeres que han sido víctimas del tráfico y de la trata de seres humanos con fines de explotación sexual, trazando de manera firme la diferencia entre tráfico de personas y trata de mujeres con fines de explotación sexual.

  9. Uso de redes neuronales artificiales para mejorar el tránsito en las vías

    Directory of Open Access Journals (Sweden)

    Edwin Mejía

    2016-11-01

    Full Text Available Este artículo pretende dar una idea de cómo las Redes Neuronales Artificiales (ANNs, una técnica de la Inteligencia Artificial (IA, se puede acoplar a resolver el problema del tráfico en las vías de la ciudad de Riobamba, provincia de Chimborazo, país Ecuador, usando cuatro semáforos estándar, a través de un ejemplo se indica el uso de componentes electrónicos como FPGAs (Field Programmable Gate array y los sensores en este campo, a través de detectar y contar autos puede dar mayor fluidez al tráfico. La vía que mayor cantidad de autos tenga se dará mayor prioridad para la luz verde. Usando el algoritmo de aprendizaje de un Perceptrón Simple.

  10. Evolution of Nova TrA 2008 into a High Mass-Accretion Rate Post-Nova

    Science.gov (United States)

    Walter, Frederick M.

    2015-06-01

    NR TrA (Nova TrA 2008) was a normal slow Fe II novae for its first year of evolution. During its third year eclipses appeared, and optical spectra revealed the presence of hot permitted lines of C IV, N V, and O VI in addition to the usual nebular lines. The light curve and spectra resemble those of the V Sge stars. The orbital period is 5.25 hours. The time-resolved spectra show a prominent S-wave in the hot lines with an amplitude of about 100 km/s. We conclude that the system is a CV with a high mass accretion rate that has persisted for some 6 years after the explosion.

  11. Coverage of the DSM-IV-TR/DSM-5 Section II Personality Disorders With the DSM-5 Dimensional Trait Model.

    Science.gov (United States)

    Rojas, Stephanie L; Widiger, Thomas A

    2017-08-01

    Section III of DSM-5, for emerging measures and models, includes a five-domain, 25-trait model, assessed by the Personality Inventory for DSM-5. A primary concern with respect to the trait model is its coverage of the DSM-IV-TR personality disorder syndromes (all of which were retained in DSM-5). The current study considered not only total scale scores of three independent measures of DSM-IV-TR personality disorders but also the coverage of each diagnostic criterion included within six personality disorders: antisocial, borderline, avoidant, dependent, narcissistic, and obsessive-compulsive. Participants were 425 community adults, all of whom had received mental health treatment (36% currently; 75% within the past year). Results provided support for the coverage of the diagnostic criteria for the antisocial, borderline, avoidant, dependent, and narcissistic personality disorders. Coverage could perhaps be improved for a few of the criteria for obsessive-compulsive personality disorder.

  12. Deep Sequencing Transcriptome Analysis of Murine Wound Healing: Effects of a Multicomponent, Multitarget Natural Product Therapy-Tr14.

    Science.gov (United States)

    St Laurent, Georges; Seilheimer, Bernd; Tackett, Michael; Zhou, Jianhua; Shtokalo, Dmitry; Vyatkin, Yuri; Ri, Maxim; Toma, Ian; Jones, Dan; McCaffrey, Timothy A

    2017-01-01

    Wound healing involves an orchestrated response that engages multiple processes, such as hemostasis, cellular migration, extracellular matrix synthesis, and in particular, inflammation. Using a murine model of cutaneous wound repair, the transcriptome was mapped from 12 h to 8 days post-injury, and in response to a multicomponent, multi-target natural product, Tr14. Using single-molecule RNA sequencing (RNA-seq), there were clear temporal changes in known transcripts related to wound healing pathways, and additional novel transcripts of both coding and non-coding genes. Tr14 treatment modulated >100 transcripts related to key wound repair pathways, such as response to wounding, wound contraction, and cytokine response. The results provide the most precise and comprehensive characterization to date of the transcriptome's response to skin damage, repair, and multicomponent natural product therapy. By understanding the wound repair process, and the effects of natural products, it should be possible to intervene more effectively in diseases involving aberrant repair.

  13. Downregulation of miR-17~92 Expression Increase Paclitaxel Sensitivity in Human Ovarian Carcinoma SKOV3-TR30 Cells via BIM Instead of PTEN

    Directory of Open Access Journals (Sweden)

    Cui Hong Bai

    2013-02-01

    Full Text Available To better understand the molecular mechanisms of paclitaxel resistance in ovarian carcinoma, we evaluated the expression of miRNAs using miRNA microarray between human ovarian carcinoma SKOV3 cells and paclitaxel resistant SKOV3-TR30 cells. Results showed that 69 miRNAs were upregulated while 102 miRNAs were downregulated in SKOV3-TR30 cells. Using real-time PCR, we further clarified that miR-17~92 was overexpressed in SKOV3-TR30 cells compared with that in SKOV3 cells. We then established stable virally transduced SKOV3-TR30-m-PTIP-Sponge all SKOV3-TR30 cells and its vector-only control SKOV3-TR30-m-PTIP-GFP cells. Real time-PCR revealed that SKOV3-TR30-m-PTIP-Sponge all cells expressed approximately 6.18-fold lower levels of miR-17~92 compared with the control group. Decreased expression of miR-17~92 resulted in cell cycle arrest in the G2/M phase and growth inhibition. After the transduction, the BIM protein level was increased in SKOV3-TR30 cells and luciferase reporter assays revealed that miR-17~92 binds directly to the 3'-UTR of BIM. Results of luciferase reporter assays accompanied with Western Blot showed that although miR-17~92 binds directly to the 3'-UTR of PTEN, the PTEN protein expression level was upregulated slightly while the result is of no statistical significance. Our results showed that miR-17~92 could be a causal factor of the downregulation of BIM in SKOV3-TR30 cells and thus induce the paclitaxel resistance in SKOV3-TR30 cells.

  14. Neuronal Ca(2+) dyshomeostasis in Huntington disease.

    Science.gov (United States)

    Giacomello, Marta; Oliveros, Juan C; Naranjo, Jose R; Carafoli, Ernesto

    2013-01-01

    The expansion of the N-terminal poly-glutamine tract of the huntingtin (Htt) protein is responsible for Huntington disease (HD). A large number of studies have explored the neuronal phenotype of HD, but the molecular aethiology of the disease is still very poorly understood. This has hampered the development of an appropriate therapeutical strategy to at least alleviate its symptoms. In this short review, we have focused our attention on the alteration of a specific cellular mechanism common to all HD models, either genetic or induced by treatment with 3-NPA, i.e. the cellular dyshomeostasis of Ca(2+). We have highlighted the direct and indirect (i.e. transcriptionally mediated) effects of mutated Htt on the maintenance of the intracellular Ca(2+) balance, the correct modulation of which is fundamental to cell survival and the disturbance of which plays a key role in the death of the cell.

  15. Conversation Analysis (CA in Primary School Classrooms

    Directory of Open Access Journals (Sweden)

    Alberto Fajardo

    2008-12-01

    Full Text Available Although CA deals with all kinds of talk produced in natural contexts, this study focuses its interest on the talk produced in some primary school classrooms. It attempts to develop the construct that CA should move significantly ahead to more practical grounds where its detailed and isolated description causes some effect in improving foreign language teaching, for example. It might be used, for instance, to promote professional development in Colombia. It plans to involve pre-service teachers initially and in-service ones later. The kind of interaction promoted by trainee teachers shows a very restricted possibility for young learners to use the language meaningfully in the classroom. Four stages are defined and suggested as a path to follow with pre-service teachers at Universidad Pedagógica y Tecnológica de Colombia –UPTC– in the Foreign Language Programme - FLP.

  16. Tröger’s Base Ladder Polymer for Membrane-Based Hydrocarbon Separation

    KAUST Repository

    Alhazmi, Abdulrahman

    2017-05-01

    The use of polymeric membranes for natural gas separation has rapidly increased during the past three decades, particularly for carbon dioxide separation from natural gas. Another valuable application is the separation of heavy hydrocarbons from methane (fuel gas conditioning), more importantly for remote area and off-shore applications. A new potential polymeric membrane that might be utilized for natural gas separations is a Tröger’s base ladder polymer (PIM-Trip-TB-2). This glassy polymeric membrane was synthesized by the polymerization reaction of 9, 10-dimethyl-2,6 (7) diaminotriptycene with dimethoxymethane. In this research, the polymer was selected due to its high surface area and highly interconnected microporous structure. Sorption isotherms of nitrogen (N2), oxygen (O¬2), methane (CH4), carbon dioxide (CO2), ethane (C2H6), propane (C3H8), and n-butane (n-C4H10) were measured at 35 °C over a range of pressures using a Hiden Intelligent Gravimetric Analyzer, IGA. The more condensable gases (C2H6, CO2, C3H8, and n-C4H10) showed high solubility due to their high affinity to the polymer matrix. The permeation coefficients were determined for various gases at 35 °C and pressure difference of 5 bar via the constant-pressure/variable-volume method. The PIM-Trip-TB-2 film exhibited high performance for several high-impact applications, such as O2/N2, H2/N2 and H2/CH4. Also, physical aging for several gases was examined by measuring the permeability coefficients at different periods of time. Moreover, a series of mixed-gas permeation tests was performed using 2 vol.% n-C4H10/98 vol.% CH4 and the results showed similar transport characteristics to other microporous polymers with pores of less than 2 nm. The work performed in this research suggested that PIM-Trip-TB-2 is suitable for the separation of: (i) higher hydrocarbons from methane and (ii) small, non-condensable gases such as O2/N2 and H2/CH4.

  17. TR-PIV measurement of the wake behind a grooved cylinder at low Reynolds number

    Science.gov (United States)

    Liu, Ying Zheng; Shi, Liu Liu; Yu, Jun

    2011-04-01

    A comparative study of the wakes behind cylinders with grooved and smooth surfaces was performed with a view to understand the wake characteristics associated with the adult Saguaro cacti. A low-speed recirculation water channel was established for the experiment; the Reynolds number, based on the free-stream velocity and cylinder diameter (D), was kept at ReD=1500. State-of-the-art time-resolved particle image velocimetry (TR-PIV) was employed to measure a total of 20 480 realizations of the wake field at a frame rate of 250 Hz, enabling a comprehensive view of the time- and phase-averaged wake pattern. In comparison to the wake behind the smooth cylinder, the length of the recirculation zone behind the grooved cylinder was extended by nearly 18.2%, yet the longitudinal velocity fluctuation intensity was considerably weakened. A global view of the peaked spectrum of the longitudinal velocity component revealed that the intermediate region for the grooved cylinder, which approximately corresponds to the transition region where the shear layer vortices interact, merge and shed before the formation of the Karman-like vortex street, was much wider than that for the smooth one. The unsteady events near St=0.3-0.4 were detected in the intermediate region behind the grooved cylinder, but no such events were found in the smooth cylinder system. Although the formation of the Karman-like vortex street was delayed by about 0.6D downstream for the grooved cylinder, no prominent difference in the vortex street region was found in the far wake for both cylinders. The Proper Orthogonal Decomposition (POD) method was used extensively to decompose the vector and swirling strength fields, which gave a close-up view of the vortices in the near wake. The first two POD modes of the swirling strength clarified the spatio-temporal characteristics of the shear layer vortices behind the grooved cylinder. The small-scale vortices superimposed on the shear layers behind the grooved cylinder

  18. Mortalidade masculina em três capitais brasileiras, 1979 a 2007

    Directory of Open Access Journals (Sweden)

    Carolina Terra de Moraes Luizaga

    2013-03-01

    Full Text Available INTRODUÇÃO: No Brasil, verifica-se maior mortalidade masculina em praticamente todas as idades e na quase totalidade das causas. OBJETIVO: Estimar e descrever a tendência da mortalidade masculina, entre 1979 e 2007, em São Paulo (SP, Rio de Janeiro (RJ e Porto Alegre (RS. MATERIAL E MÉTODO: As populações de estudo referem-se aos residentes nas três capitais, nos triênios 1979/81, 1990/92, 1999/2001 e 2005/07 e respectivos óbitos. As fontes de dados incluíram Instituto Brasileiro de Geografia e Estatística e Sistemas de Informações em Saúde do Brasil. Calcularam-se os coeficientes de mortalidade gerais e específicos (brutos e padronizados. RESULTADOS: Verificaram-se declínio da proporção de crianças e de jovens e crescimento da proporção de idosos. Até 24 anos, os homens predominaram na população; a partir daí observaram-se maiores participações femininas e razões de sexos cada vez mais baixas, evidenciando, entre idosos, maior presença de mulheres, fato associado à elevada mortalidade masculina. Houve perda intensa de jovens por causas externas. Em 2005/07, este grupo correspondeu à principal causa de morte masculina até a faixa de 40-44 anos. Nos grupos etários seguintes, as doenças circulatórias foram a principal causa. CONSIDERAÇÕES FINAIS: As localidades evidenciam características de cidades em desenvolvimento, com redução da fecundidade, aumento da sobrevivência e envelhecimento populacional. As estimativas do elevado risco de morrer dos homens tornam clara sua vulnerabilidade, demandando ações que possibilitem redução da mortalidade por causas evitáveis, eliminando comportamentos de risco e incentivando hábitos saudáveis. Só assim haverá aumento da esperança de vida e redução das diferenças entre as mortalidades feminina e masculina.

  19. A mobilidade humana na tríplice fronteira: Peru, Brasil e Colômbia

    Directory of Open Access Journals (Sweden)

    Márcia Maria de Oliveira

    2006-08-01

    Full Text Available OS MOVIMENTOS migratórios nas fronteiras amazônicas ainda são pouco abordados, tanto pela academia quanto pelas instituições que lidam com a temática da mi-gração. Há elementos novos que configuram características peculiares à mobilidade humana na tríplice fronteira Peru-Colômbia-Brasil que merecem uma abordagem mais profunda do ponto de vista dos estudos migratórios. Atualmente, há fluxos conside-ráveis de migração internacional nessa região adentrando na fronteira brasileira, desafiando o Estado brasileiro a implementar uma política migratória que consiga lidar com fenômenos, tais como a presença de peruanos em situação irregular em território brasileiro, a mobilidade dos povos indígenas nas regiões de fronteira e ainda, mais recentemente, a entrada crescente de colombianos desplazados pela guerrilha interna que pedem refúgio ao Estado brasileiro.THE MIGRATORY mobility in the Amazonian borders is less than enough investigated, neither by the academy nor by the institutions that deal with the migration question. There are new elements that depict peculiar characteristics to the human mobility in the triple border of Peru, Colombia and Brazil, that deserve a deeper investigation from the view point of the migratory studies. At the moment, it has conside-rable flows of international migration in this region moving in the Brazilian border, challenging the Brazilian State to implement a migratory policy that is able to deal with the phenomena such as the presence of Peruvians in an irregular situation in Brazilian territory, the mobility of the indigenous peoples in the border regions and still, more recently, the increasing entrance of the Colombians desplazados through the internal guerrilla who ask for shelter to the Brazilian State.

  20. Razão de Perdas de Solo sob cultivo de três leguminosas

    Directory of Open Access Journals (Sweden)

    P. C. Nascimento

    1999-03-01

    Full Text Available Um experimento sobre perdas de água e solo por erosão foi desenvolvido, durante seis anos agrícolas (1973/74 a 1978/79, no Centro Experimental de Campinas do Instituto Agronômico do estado de São Paulo, Brasil, em um Typic Haplortox, com vistas em avaliar a Razão de Perdas de Solo (RPS para três leguminosas utilizadas como adubos verdes. As leguminosas estudadas foram a Crotalaria juncea L. (crotalária; Stizolobium Aterrinum Piper et Tracy (mucuna-preta e Dolichos lablab L. (labelabe. Para determinar a razão de perdas de solo, foram estabelecidos quatro períodos das culturas isolados de 0-30, 30-60, 60-90 e 60-120 dias após a semeadura e períodos cumulativos de 0-30, 0-60, 0-90 e 0-120 dias após a semeadura. Os resultados obtidos mostraram maior proteção oferecida pela mucuna-preta à erosão, com valores de RPS de 0,28; 0,37; 0,17; e 0,09 para os períodos isolados de 0-30, 30-60; 60-90 e 60-120 dias após a semeadura. O labelabe apresentou, para a mesma situação, os valores de 0,50; 0,45; 0,27 e 0,19; enquanto, para a crotalária, os valores foram de 0,30; 0,42; 0,26 e 0,24. Os resultados mostraram proteção mais efetiva do solo à erosão a partir dos 60 dias após a semeadura, por parte das espécies avaliadas. A mucuna-preta apresentou maior efetividade na proteção do solo, seguida da crotalária, especialmente no primeiro período, e do labelabe, no estádio mais próximo ao florescimento.