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Sample records for underway chromatographic system

  1. Dual liquid and gas chromatograph system

    Science.gov (United States)

    Gay, D.D.

    A chromatographic system is described that utilizes one detection system for gas chromatographic and micro-liquid chromatographic determinations. The detection system is a direct-current, atmospheric-pressure, helium plasma emission spectrometer. The detector utilizes a nontransparent plasma source unit which contains the plasma region and two side-arms which receive effluents from the micro-liquid chromatograph and the gas chromatograph. The dual nature of this chromatographic system offers: (1) extreme flexibility in the samples to be examined; (2) extreme low sensitivity; (3) element selectivity; (4) long-term stability; (5) direct correlation of data from the liquid and gas samples; (6) simpler operation than with individual liquid and gas chromatographs, each with different detection systems; and (7) cheaper than a commercial liquid chromatograph and a gas chromatograph.

  2. Modeling Aquatic Toxicity through Chromatographic Systems.

    Science.gov (United States)

    Fernández-Pumarega, Alejandro; Amézqueta, Susana; Farré, Sandra; Muñoz-Pascual, Laura; Abraham, Michael H; Fuguet, Elisabet; Rosés, Martí

    2017-08-01

    Environmental risk assessment requires information about the toxicity of the growing number of chemical products coming from different origins that can contaminate water and become toxicants to aquatic species or other living beings via the trophic chain. Direct toxicity measurements using sensitive aquatic species can be carried out but they may become expensive and ethically questionable. Literature refers to the use of chromatographic measurements that correlate to the toxic effect of a compound over a specific aquatic species as an alternative to get toxicity information. In this work, we have studied the similarity in the response of the toxicity to different species and we have selected eight representative aquatic species (including tadpoles, fish, water fleas, protozoan, and bacteria) with known nonspecific toxicity to chemical substances. Next, we have selected four chromatographic systems offering good perspectives for surrogation of the eight selected aquatic systems, and thus prediction of toxicity from the chromatographic measurement. Then toxicity has been correlated to the chromatographic retention factor. Satisfactory correlation results have been obtained to emulate toxicity in five of the selected aquatic species through some of the chromatographic systems. Other aquatic species with similar characteristics to these five representative ones could also be emulated by using the same chromatographic systems. The final aim of this study is to model chemical products toxicity to aquatic species by means of chromatographic systems to reduce in vivo testing.

  3. Performance of chromatographic systems to model soil-water sorption.

    Science.gov (United States)

    Hidalgo-Rodríguez, Marta; Fuguet, Elisabet; Ràfols, Clara; Rosés, Martí

    2012-08-24

    A systematic approach for evaluating the goodness of chromatographic systems to model the sorption of neutral organic compounds by soil from water is presented in this work. It is based on the examination of the three sources of error that determine the overall variance obtained when soil-water partition coefficients are correlated against chromatographic retention factors: the variance of the soil-water sorption data, the variance of the chromatographic data, and the variance attributed to the dissimilarity between the two systems. These contributions of variance are easily predicted through the characterization of the systems by the solvation parameter model. According to this method, several chromatographic systems besides the reference octanol-water partition system have been selected to test their performance in the emulation of soil-water sorption. The results from the experimental correlations agree with the predicted variances. The high-performance liquid chromatography system based on an immobilized artificial membrane and the micellar electrokinetic chromatography systems of sodium dodecylsulfate and sodium taurocholate provide the most precise correlation models. They have shown to predict well soil-water sorption coefficients of several tested herbicides. Octanol-water partitions and high-performance liquid chromatography measurements using C18 columns are less suited for the estimation of soil-water partition coefficients. Copyright © 2012 Elsevier B.V. All rights reserved.

  4. Acquisition of an Underway CTD System for the Flow Encountering Abrupt Topography DRI

    Science.gov (United States)

    2015-09-30

    Acquisition of an Underway CTD System for the Flow Encountering Abrupt Topography DRI T. M. Shaun Johnston Scripps Institution of Oceanography...westward flow in the North Equatorial Current (NEC) encounters tall, steep, submarine topography and islands. During the Flow Encountering Abrupt... Topography (FLEAT) DRI, investigators will determine: • Whether appreciable energy/momentum is lost from the large-scale NEC flow to smaller scales and

  5. Large scale gas chromatographic demonstration system for hydrogen isotope separation

    International Nuclear Information System (INIS)

    Cheh, C.H.

    1988-01-01

    A large scale demonstration system was designed for a throughput of 3 mol/day equimolar mixture of H,D, and T. The demonstration system was assembled and an experimental program carried out. This project was funded by Kernforschungszentrum Karlsruhe, Canadian Fusion Fuel Technology Projects and Ontario Hydro Research Division. Several major design innovations were successfully implemented in the demonstration system and are discussed in detail. Many experiments were carried out in the demonstration system to study the performance of the system to separate hydrogen isotopes at high throughput. Various temperature programming schemes were tested, heart-cutting operation was evaluated, and very large (up to 138 NL/injection) samples were separated in the system. The results of the experiments showed that the specially designed column performed well as a chromatographic column and good separation could be achieved even when a 138 NL sample was injected

  6. Standard Format for Chromatographic-polarimetric System small samples assessment

    International Nuclear Information System (INIS)

    Naranjo, S.; Fajer, V.; Fonfria, C.; Patinno, R.

    2012-01-01

    The treatment of samples containing optically active substances to be evaluated as part of quality control of raw material entering industrial process, and also during the modifications exerted on it to obtain the desired final composition is still and unsolved problem for many industries. That is the case of sugarcane industry. Sometimes the troubles implied are enlarged because samples to be evaluated are not bigger than one milliliter. Reduction of gel beds in G-10 and G-50 chromatographic columns having an inner diameter of 16 mm, instead of 25, and bed heights adjustable to requirements by means of sliding stoppers to increase analytical power were evaluated with glucose and sucrose standards in concentrations from 1 to 10 g/dL, using aliquots of 1 ml without undesirable dilutions that could affect either detection or chromatographic profile. Assays with seaweed extracts gave good results that are shown. It is established the advantage to know concentration of a separated substance by the height of its peak and the savings in time and reagents resulting . Sample expanded uncertainty in both systems is compared. It is also presented several programs for data acquisition, storing and processing. (Author)

  7. Temperature Control of Gas Chromatograph Based on Switched Delayed System Techniques

    Directory of Open Access Journals (Sweden)

    Xiao-Liang Wang

    2014-01-01

    Full Text Available We address the temperature control problem of the gas chromatograph. We model the temperature control system of the gas chromatograph into a switched delayed system and analyze the stability by common Lyapunov functional technique. The PI controller parameters can be given based on the proposed linear matrix inequalities (LMIs condition and the designed controller can make the temperature of gas chromatograph track the reference signal asymptotically. An experiment is given to illustrate the effectiveness of the stability criterion.

  8. Automated chromatographic laccase-mediator-system activity assay.

    Science.gov (United States)

    Anders, Nico; Schelden, Maximilian; Roth, Simon; Spiess, Antje C

    2017-08-01

    To study the interaction of laccases, mediators, and substrates in laccase-mediator systems (LMS), an on-line measurement was developed using high performance anion exchange chromatography equipped with a CarboPac™ PA 100 column coupled to pulsed amperometric detection (HPAEC-PAD). The developed method was optimized for overall chromatographic run time (45 to 120 min) and automated sample drawing. As an example, the Trametes versicolor laccase induced oxidation of 1-(3,4-dimethoxyphenyl)-2-(2-methoxyphenoxy)-1,3-dihydroxypropane (adlerol) using 1-hydroxybenzotriazole (HBT) as mediator was measured and analyzed on-line. Since the Au electrode of the PAD detects only hydroxyl group containing substances with a limit of detection being in the milligram/liter range, not all products are measureable. Therefore, this method was applied for the quantification of adlerol, and-based on adlerol conversion-for the quantification of the LMS activity at a specific T. versicolor laccase/HBT ratio. The automated chromatographic activity assay allowed for a defined reaction start of all laccase-mediator-system reactions mixtures, and the LMS reaction progress was automatically monitored for 48 h. The automatization enabled an integrated monitoring overnight and over-weekend and minimized all manual errors such as pipetting of solutions accordingly. The activity of the LMS based on adlerol consumption was determined to 0.47 U/mg protein for a laccase/mediator ratio of 1.75 U laccase/g HBT. In the future, the automated method will allow for a fast screening of combinations of laccases, mediators, and substrates which are efficient for lignin modification. In particular, it allows for a fast and easy quantification of the oxidizing activity of an LMS on a lignin-related substrate which is not covered by typical colorimetric laccase assays. ᅟ.

  9. Effect of chromatographic separation on ASP system interface tension and the countermeasures

    Directory of Open Access Journals (Sweden)

    Jiawei REN

    2016-06-01

    Full Text Available Because of the existing chromatographic separation phenomenon, ASP flooding changes original nature of the system. Therefore, in laboratory ultra-low interfacial tension ASP system is preferred for sand packs flow experiment to research on the effect of chromatographic separation on ASP system interface tension. The two parameters of "breakthrough time" and "output difference" are used to describe the degree of chromatographic separation, and the produced fluid interfacial tensions at the outlet end at 120 min is measured. The research shows that there exists chromatographic separation between three chemicals of ASP system, with first polymer breakthrough and finally surfactant breakthrough; there is most serious chromatographic separation between surfactant and polymer, while minimum chromatographic separation between alkali and polymer; chromatographic separation makes ASP interfacial tension increase from 10-3 magnitude to 10-2 magnitude, affecting flooding effect of ASP system. Thus, reducing the loss of surfactant in the formation will be the main measure to reduce the degree of chromatographic separation. Using sophorolipid as sacrificial agent to replace part of the surfactant injected into formation before ASP system can effectively reduce the impact of chromatography separation and more effectively improve the ultimate recovery ratio.

  10. Spectrophotometric high-precision seawater pH determination for use in underway measuring systems

    Directory of Open Access Journals (Sweden)

    S. Aßmann

    2011-10-01

    Full Text Available Autonomous sensors are required for a comprehensive documentation of the changes in the marine carbon system and thus to differentiate between its natural variability and anthropogenic impacts. Spectrophotometric determination of pH – a key variable of the seawater carbon system – is particularly suited to achieve precise and drift-free measurements. However, available spectrophotometric instruments are not suitable for integration into automated measurement systems (e.g. FerryBox since they do not meet the major requirements of reliability, stability, robustness and moderate cost. Here we report on the development and testing of a~new indicator-based pH sensor that meets all of these requirements. This sensor can withstand the rough conditions during long-term deployments on ships of opportunity and is applicable to the open ocean as well as to coastal waters with a complex matrix and highly variable conditions. The sensor uses a high resolution CCD spectrometer as detector connected via optical fibers to a custom-made cuvette designed to reduce the impact of air bubbles. The sample temperature can be precisely adjusted (25 °C ± 0.006 °C using computer-controlled power supplies and Peltier elements thus avoiding the widely used water bath. The overall setup achieves a measurement frequency of 1 min−1 with a precision of ±0.0007 pH units, an average offset of +0.0005 pH units to a reference system, and an offset of +0.0081 pH units to a certified standard buffer. Application of this sensor allows monitoring of seawater pH in autonomous underway systems, providing a key variable for characterization and understanding of the marine carbon system.

  11. Chromatographic generator systems for the actinides and natural decay series elements

    International Nuclear Information System (INIS)

    McAlister, D.R.; Horwitz, E.P.

    2011-01-01

    This work describes chromatographic radionuclide generator systems for the production of actinides and natural decay series elements. The generator systems begin with alpha emitting parent radioisotopes with half-lives (T 1/2 ) of greater than one year and produce alpha or beta emitting radioisotopes with half-lives of hours to days. Chromatographic systems were chosen to minimize radiolytic damage to chromatographic supports, preserve the parent activity for repeated use, provide high purity daughter radionuclide tracers, and to minimize or eliminate the need for evaporation of solutions of the parent or daughter nuclides. Useful secondary separations involving the daughters of the initial parent radionuclide are also described. Separation systems for 210 Bi, 210 Po, 211 Pb, 212 Pb, 223 Ra, 224 Ra, 225 Ra, 225 Ac, 227 Th, 228 Th, 231 Th, 234 Th, and 239 Np are outlined in detail. (orig.)

  12. Evaluation of the suitability of chromatographic systems to predict human skin permeation of neutral compounds.

    Science.gov (United States)

    Hidalgo-Rodríguez, Marta; Soriano-Meseguer, Sara; Fuguet, Elisabet; Ràfols, Clara; Rosés, Martí

    2013-12-18

    Several chromatographic systems (three systems of high-performance liquid chromatography and two micellar electrokinetic chromatography systems) besides the reference octanol-water partition system are evaluated by a systematic procedure previously proposed in order to know their ability to model human skin permeation. The precision achieved when skin-water permeability coefficients are correlated against chromatographic retention factors is predicted within the framework of the solvation parameter model. It consists in estimating the contribution of error due to the biological and chromatographic data, as well as the error coming from the dissimilarity between the human skin permeation and the chromatographic systems. Both predictions and experimental tests show that all correlations are greatly affected by the considerable uncertainty of the skin permeability data and the error associated to the dissimilarity between the systems. Correlations with much better predictive abilities are achieved when the volume of the solute is used as additional variable, which illustrates the main roles of both lipophilicity and size of the solute to penetrate through the skin. In this way, the considered systems are able to give precise estimations of human skin permeability coefficients. In particular, the HPLC systems with common C18 columns provide the best performances in emulating the permeation of neutral compounds from aqueous solution through the human skin. As a result, a methodology based on easy, fast, and economical HPLC measurements in a common C18 column has been developed. After a validation based on training and test sets, the method has been applied with good results to the estimation of skin permeation of several hormones and pesticides. Copyright © 2013 Elsevier B.V. All rights reserved.

  13. Simple gas chromatographic system for analysis of microbial respiratory gases

    Science.gov (United States)

    Carle, G. C.

    1972-01-01

    Dual column ambient temperature system, consisting of pair of capillary columns, microbead thermistor detector and micro gas-sampling valve, is used in remote life-detection equipment for space experiments. Performance outweighs advantage gained by utilizing single-column systems to reduce weight, conserve carrier gas and operate at lower power levels.

  14. Autonomous gas chromatograph system for Thermal Enhanced Vapor Extraction System (TEVES) proof of concept demonstration

    International Nuclear Information System (INIS)

    Peter, F.J.; Laguna, G.R.

    1996-09-01

    An autonomous gas chromatograph system was designed and built to support the Thermal Enhanced Vapor Extraction System (TEVES) demonstration. TEVES is a remediation demonstration that seeks to enhance an existing technology (vacuum extraction) by adding a new technology (soil heating). A pilot scale unit was set up at one of the organic waste disposal pits at the Sandia National Laboratories Chemical Waste Landfill (CWL) in Tech Area 3. The responsibility for engineering a major part of the process instrumentation for TEVES belonged to the Manufacturing Control Subsystems Department. The primary mission of the one-of-a-kind hardware/software system is to perform on-site gas sampling and analysis to quantify a variety of volatile organic compounds (VOCs) from various sources during TEVES operations. The secondary mission is to monitor a variety of TEVES process physical parameters such as extraction manifold temperature, pressure, humidity, and flow rate, and various subsurface pressures. The system began operation in September 1994 and was still in use on follow-on projects when this report was published

  15. A Gas Chromatographic System for the Detection of Ethylene Gas Using Ambient Air as a Carrier Gas.

    Science.gov (United States)

    Zaidi, Nayyer Abbas; Tahir, Muhammad Waseem; Vellekoop, Michael J; Lang, Walter

    2017-10-07

    Ethylene gas is a naturally occurring gas that has an influence on the shelf life of fruit during their transportation in cargo ships. An unintentional exposure of ethylene gas during transportation results in a loss of fruit. A gas chromatographic system is presented here for the detection of ethylene gas. The gas chromatographic system was assembled using a preconcentrator, a printed 3D printed gas chromatographic column, a humidity sensor, solenoid valves, and an electrochemical ethylene gas sensor. Ambient air was used as a carrier gas in the gas chromatographic system. The flow rate was fixed to 10 sccm. It was generated through a mini-pump connected in series with a mass flow controller. The metal oxide gas sensor is discussed with its limitation in ambient air. The results show the chromatogram obtained from metal oxide gas sensor has low stability, drifts, and has uncertain peaks, while the chromatogram from the electrochemical sensor is stable and precise. Furthermore, ethylene gas measurements at higher ppb concentration and at lower ppb concentration were demonstrated with the electrochemical ethylene gas sensor. The system separates ethylene gas and humidity. The chromatograms obtained from the system are stable, and the results are 1.2% repeatable in five similar measurements. The statistical calculation of the gas chromatographic system shows that a concentration of 2.3 ppb of ethylene gas can be detected through this system.

  16. Optical Remote Sensing Algorithm Validation using High-Frequency Underway Biogeochemical Measurements in Three Large Global River Systems

    Science.gov (United States)

    Kuhn, C.; Richey, J. E.; Striegl, R. G.; Ward, N.; Sawakuchi, H. O.; Crawford, J.; Loken, L. C.; Stadler, P.; Dornblaser, M.; Butman, D. E.

    2017-12-01

    More than 93% of the world's river-water volume occurs in basins impacted by large dams and about 43% of river water discharge is impacted by flow regulation. Human land use also alters nutrient and carbon cycling and the emission of carbon dioxide from inland reservoirs. Increased water residence times and warmer temperatures in reservoirs fundamentally alter the physical settings for biogeochemical processing in large rivers, yet river biogeochemistry for many large systems remains undersampled. Satellite remote sensing holds promise as a methodology for responsive regional and global water resources management. Decades of ocean optics research has laid the foundation for the use of remote sensing reflectance in optical wavelengths (400 - 700 nm) to produce satellite-derived, near-surface estimates of phytoplankton chlorophyll concentration. Significant improvements between successive generations of ocean color sensors have enabled the scientific community to document changes in global ocean productivity (NPP) and estimate ocean biomass with increasing accuracy. Despite large advances in ocean optics, application of optical methods to inland waters has been limited to date due to their optical complexity and small spatial scale. To test this frontier, we present a study evaluating the accuracy and suitability of empirical inversion approaches for estimating chlorophyll-a, turbidity and temperature for the Amazon, Columbia and Mississippi rivers using satellite remote sensing. We demonstrate how riverine biogeochemical measurements collected at high frequencies from underway vessels can be used as in situ matchups to evaluate remotely-sensed, near-surface temperature, turbidity, chlorophyll-a derived from the Landsat 8 (NASA) and Sentinel 2 (ESA) satellites. We investigate the use of remote sensing water reflectance to infer trophic status as well as tributary influences on the optical characteristics of the Amazon, Mississippi and Columbia rivers.

  17. A chromatographic determination of water in non-aqueous phases of solvent extraction systems

    International Nuclear Information System (INIS)

    Lyle, S.J.; Smith, D.B.

    1975-01-01

    The disadvantages of the Karl Fischer method for the determination of water in the non-aqueous phases of solvent extraction systems are pointed out, and a gas chromatographic method is described which is claimed to be potentially capable of overcoming these disadvantages. The method, as described, was developed to satisfy conditions relevant to measurement of the transfer rate of water from an aqueous phase into tri-n-butylphosphate in toluene, but it can be used for water determination in other solvent extraction systems. The apparatus used is described in detail. The concentration of water in water-saturated TBP was found to be 3.56 mol/litre, compared with a value of 3.55 obtained by Karl Fischer titration and previous literature values of 3.59 and 3.57. Measurements of water content in benzene solutions of long chain alkylamines were also sucessfully carried out. (U.K.)

  18. A Gas Chromatographic Continuous Emissions Monitoring System for the Determination of VOCs and HAPs.

    Science.gov (United States)

    Coleman, William M; Gordon, Bert M

    1996-01-01

    This article describes a new gas chromatography-based emissions monitoring system for measuring volatile organic compounds (VOCs) and hazardous air pollutants (HAPs). The system is composed of a dual-column gas chromatograph equipped with thermal conductivity detectors, in which separation is optimized for fast chromatography. The system has the necessary valving for stream selection, which allows automatic calibration of the system at predetermined times and successive measurement of individual VOCs before and after a control device. Nine different VOCs (two of which are HAPs), plus methane (CH4) and carbon dioxide (CO2) are separated and quantified every two minutes. The accuracy and precision of this system has been demonstrated to be greater than 95%. The system employs a mass flow measurement device and also calculates and displays processed emission data, such as control device efficiency and total weight emitted during given time periods. Two such systems have been operational for one year in two separate gravure printing facilities; minimal upkeep is required, about one hour per month. One of these systems, used before and after a carbon adsorber, has been approved by the pertinent local permitting authority.

  19. [Research on the application of grey system theory in the pattern recognition for chromatographic fingerprints of traditional Chinese medicine].

    Science.gov (United States)

    Wei, Hang; Lin, Li; Zhang, Yuan; Wang, Lianjing; Chen, Qinqun

    2013-02-01

    A model based on grey system theory was proposed for pattern recognition in chromatographic fingerprints (CF) of traditional Chinese medicine (TCM). The grey relational grade among the data series of each testing CF and the ideal CF was obtained by entropy and norm respectively, then the principle of "maximal matching degree" was introduced to make judgments, so as to achieve the purpose of variety identification and quality evaluation. A satisfactory result in the high performance liquid chromatographic (HPLC) analysis of 56 batches of different varieties of Exocarpium Citrus Grandis was achieved with this model. The errors in the chromatographic fingerprint analysis caused by traditional similarity method or grey correlation method were overcome, as the samples of Citrus grandis 'Tomentosa' and Citrus grandis (L.) Osbeck were correctly distinguished in the experiment. Furthermore in the study on the variety identification of Citrus grandis 'Tomentosa', the recognition rates were up to 92.85%, although the types and the contents of the chemical compositions of the samples were very close. At the same time, the model had the merits of low computation complexity and easy operation by computer programming. The research indicated that the grey system theory has good applicability to pattern recognition in the chromatographic fingerprints of TCM.

  20. Conceptual design of hydrogen isotopes chromatographic separation system with super large capacity

    International Nuclear Information System (INIS)

    Xie Bo; Weng Kuiping; Liu Yunnu; Hou Jianping

    2012-01-01

    A super large capacity hydrogen isotopes separation system, including total plan, unit (including making and purification of gas, three-grade chromatographic columns, gas loop and auto-control, and carrier recovery) and experimental scheme, had been designed on the basis of a series of hydrogen-deuterium experiments by temperature programmed de- sorption. The characteristic of the system was that desorption kinetic parameters could be directly calculated from the hydrogen isotope separation desorption spectra information. In other words, the complicated dynamic process of separation could be described by the desorption rate equation, shape parameter and desorption activation energy calculation on the condition of the experimental data and appropriate assumptions (equilibrium and adsorption, uniform surface). In previous work, an experimental series of operation to verify the successive enrichment of D 2 from a H 2 -D 2 mixture, the production of the deuterium from natural hydrogen and the recovery of tritium such as from the nuclear heavy-water were carried out using MS5A at 77 K. This work was only conceptual design, so it was necessary to identify the availability of super large capacity system by experiment. (authors)

  1. [Similarity system theory to evaluate similarity of chromatographic fingerprints of traditional Chinese medicine].

    Science.gov (United States)

    Liu, Yongsuo; Meng, Qinghua; Jiang, Shumin; Hu, Yuzhu

    2005-03-01

    The similarity evaluation of the fingerprints is one of the most important problems in the quality control of the traditional Chinese medicine (TCM). Similarity measures used to evaluate the similarity of the common peaks in the chromatogram of TCM have been discussed. Comparative studies were carried out among correlation coefficient, cosine of the angle and an improved extent similarity method using simulated data and experimental data. Correlation coefficient and cosine of the angle are not sensitive to the differences of the data set. They are still not sensitive to the differences of the data even after normalization. According to the similarity system theory, an improved extent similarity method was proposed. The improved extent similarity is more sensitive to the differences of the data sets than correlation coefficient and cosine of the angle. And the character of the data sets needs not to be changed compared with log-transformation. The improved extent similarity can be used to evaluate the similarity of the chromatographic fingerprints of TCM.

  2. Evaluation of selectivity in homologous multimodal chromatographic systems using in silico designed antibody fragment libraries.

    Science.gov (United States)

    Karkov, Hanne Sophie; Woo, James; Krogh, Berit Olsen; Ahmadian, Haleh; Cramer, Steven M

    2015-12-24

    This study describes the in silico design, surface property analyses, production and chromatographic evaluations of a diverse set of antibody Fab fragment variants. Based on previous findings, we hypothesized that the complementarity-determining regions (CDRs) constitute important binding sites for multimodal chromatographic ligands. Given that antibodies are highly diversified molecules and in particular the CDRs, we set out to examine the generality of this result. For this purpose, four different Fab fragments with different CDRs and/or framework regions of the variable domains were identified and related variants were designed in silico. The four Fab variant libraries were subsequently generated by site-directed mutagenesis and produced by recombinant expression and affinity purification to enable examination of their chromatographic retention behavior. The effects of geometric re-arrangement of the functional moieties on the multimodal resin ligands were also investigated with respect to Fab variant retention profiles by comparing two commercially available multimodal cation-exchange ligands, Capto MMC and Nuvia cPrime, and two novel multimodal ligand prototypes. Interestingly, the chromatographic data demonstrated distinct selectivity trends between the four Fab variant libraries. For three of the Fab libraries, the CDR regions appeared as major binding sites for all multimodal ligands. In contrast, the fourth Fab library displayed a distinctly different chromatographic behavior, where Nuvia cPrime and related multimodal ligand prototypes provided markedly improved selectivity over Capto MMC. Clearly, the results illustrate that the discriminating power of multimodal ligands differs between different Fab fragments. The results are promising indications that multimodal chromatography using the appropriate multimodal ligands can be employed in downstream bioprocessing for challenging selective separation of product related variants. Copyright © 2015 Elsevier B

  3. Core-shell in liquid chromatography: application for determining sulphonamides in feed and meat using conventional chromatographic systems

    Directory of Open Access Journals (Sweden)

    Antonio Armentano

    2016-12-01

    Full Text Available A C18 column packed with core-shell particles was used for the chromatographic separation of sulphonamides in feed and meat by a conventional high performance liquid chromatography system coupled with a diode array detector. Two analytical methods, already used in our laboratory, have been modified without any changes in the extraction and clean-up steps and in the liquid chromatography instrumentation. Chromatographic conditions applied on a traditional 5-μm column have been optimized on a column packed with 2.6 μm core-shell particles. A binary mobile phase [acetate buffer solution at pH 4.50 and a mixture of methanol acetonitrile 50: 50 (v/v] was employed in gradient mode at the flow rate of 1.2 mL with an injection volume of 6 μL. These chromatographic conditions allow the separation of 13 sulphonamides with an entire run of 13 minutes. Preliminary studies have been carried out comparing blanks and spiked samples of feed and meat. A good resolution and the absence of interferences were achieved in chromatograms for both matrices. Since no change was made to the sample preparation, the optimized method does not require a complete revalidation and can be used to make routine analysis faster.

  4. Estimations of temperature deviations in chromatographic columns using isenthalpic plots. I. Theory for isocratic systems.

    Science.gov (United States)

    Tarafder, Abhijit; Iraneta, Pamela; Guiochon, Georges; Kaczmarski, Krzysztof; Poe, Donald P

    2014-10-31

    We propose to use constant enthalpy or isenthalpic diagrams as a tool to estimate the extent of the temperature variations caused by the mobile phase pressure drop along a chromatographic column, e.g. of its cooling in supercritical fluid and its heating in ultra-performance liquid chromatography. Temperature strongly affects chromatographic phenomena. Any of its variations inside the column, whether intended or not, can lead to significant changes in separation performance. Although instruments use column ovens in order to keep constant the column temperature, operating conditions leading to a high pressure drop may cause significant variations of the column temperature, both in the axial and the radial directions, from the set value. Different ways of measuring these temperature variations are available but they are too inconvenient to be employed in many practical situations. In contrast, the thermodynamic plot-based method that we describe here can easily be used with only a ruler and a pencil. They should be helpful in developing methods or in analyzing results in analytical laboratories. Although the most effective application area for this approach should be SFC (supercritical fluid chromatography), it can be applied to any chromatographic conditions in which temperature variations take place along the column due to the pressure drop, e.g. in ultra-high pressure liquid chromatography (UHPLC). The method proposed here is applicable to isocractic conditions only. Copyright © 2014 Elsevier B.V. All rights reserved.

  5. Chromatographic methods

    International Nuclear Information System (INIS)

    Marhol, M.; Stary, J.

    1975-01-01

    The characteristics are given of chromatographic separation and the methods are listed. Methods and data on materials used in partition, adsorption, precipitation and ion exchange chromatography are listed and conditions are described under which ion partition takes place. Special attention is devoted to ion exchange chromatography where tables are given to show the course of values of the partition coefficients of different ions in dependence on the concentration of agents and the course of equilibrium sorptions on different materials in dependence on the solution pH. A theoretical analysis is given and the properties of the most widely used ion exchangers are listed. Experimental conditions and apparatus used for each type of chromatography are listed. (L.K.)

  6. Partial pressure (or fugacity) of carbon dioxide, salinity and other variables collected from underway - surface observations using gas chromatograph and other instruments from the LILLOOET in the Coastal Waters of SE Alaska, Coral Sea and others from 1988-02-04 to 1988-02-20 (NODC Accession 0000439)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0000439 includes chemical, meteorological, physical and underway - surface data collected from LILLOOET in the Coastal Waters of SE Alaska, Coral Sea,...

  7. An automated multidimensional preparative gas chromatographic system for isolation and enrichment of trace amounts of xenon from ambient air.

    Science.gov (United States)

    Larson, Tuula; Östman, Conny; Colmsjö, Anders

    2011-04-01

    The monitoring of radioactive xenon isotopes is one of the principal methods for the detection of nuclear explosions in order to identify clandestine nuclear testing. In this work, a miniaturized, multiple-oven, six-column, preparative gas chromatograph was constructed in order to isolate trace quantities of radioactive xenon isotopes from ambient air, utilizing nitrogen as the carrier gas. The multidimensional chromatograph comprised preparative stainless steel columns packed with molecular sieves, activated carbon, and synthetic carbon adsorbents (e.g., Anasorb®-747 and Carbosphere®). A combination of purification techniques--ambient adsorption, thermal desorption, back-flushing, thermal focusing, and heart cutting--was selectively optimized to produce a well-defined xenon peak that facilitated reproducible heart cutting and accurate quantification. The chromatographic purification of a sample requires approximately 4 h and provides complete separation of xenon from potentially interfering components (such as water vapor, methane, carbon dioxide, and radon) with recovery and accuracy close to 100%. The preparative enrichment process isolates and concentrates a highly purified xenon gas fraction that is suitable for subsequent ultra-low-level γ-, ß/γ-spectroscopic or high-resolution mass spectrometric measurement (e.g., to monitor the gaseous fission products of nuclear explosions at remote locations). The Xenon Processing Unit is a free-standing, relatively lightweight, and transportable system that can be interfaced to a variety of sampling and detection systems. It has a relatively inexpensive, rugged, and compact modular (19-inch rack) design that provides easy access to all parts for maintenance and has a low power requirement.

  8. Development of new process network for gas chromatograph and analyzers connected with SCADA system and Digital Control Computers at Cernavoda NPP Unit 1

    International Nuclear Information System (INIS)

    Deneanu, Cornel; Popa Nemoiu, Dragos; Nica, Dana; Bucur, Cosmin

    2007-01-01

    The continuous monitoring of gas mixture concentrations (deuterium/ hydrogen/oxygen/nitrogen) accumulated in 'Moderator Cover Gas', 'Liquid Control Zone' and 'Heat Transport D 2 O Storage Tank Cover Gas', as well as the continuous monitoring of Heavy Water into Light Water concentration in 'Boilers Steam', 'Boilers Blown Down', 'Moderator heat exchangers', and 'Recirculated Water System', sensing any leaks of Cernavoda NPP U1 led to requirement of developing a new process network for gas chromatograph and analyzers connected to the SCADA system and Digital Control Computers of Cernavoda NPP Unit 1. In 2005 it was designed and implemented the process network for gas chromatograph which connected the gas chromatograph equipment to the SCADA system and Digital Control Computers of the Cernavoda NPP Unit 1. Later this process network for gas chromatograph has been extended to connect the AE13 and AE14 Fourier Transform Infrared (FTIR) analyzers with either. The Gas Chromatograph equipment measures with best accuracy the mixture gases (deuterium/ hydrogen/oxygen/nitrogen) concentration. The Fourier Transform Infrared (FTIR) AE13 and AE14 Analyzers measure the Heavy Water into Light Water concentration in Boilers Steam, Boilers BlownDown, Moderator heat exchangers, and Recirculated Water System, monitoring and signaling any leaks. The Gas Chromatograph equipment and Fourier Transform Infrared (FTIR) AE13 and AE14 Analyzers use the new OPC (Object Link Embedded for Process Control) technologies available in ABB's VistaNet network for interoperability with automation equipment. This new process network has interconnected the ABB chromatograph and Fourier Transform Infrared analyzers with plant Digital Control Computers using new technology. The result was an increased reliability and capability for inspection and improved system safety

  9. Detection system for a gas chromatograph. [. cap alpha. -methylnaphthalene,. beta. -methylnapthalene

    Science.gov (United States)

    Hayes, J.M.; Small, G.J.

    1982-04-26

    A method and apparatus are described for the quantitative analysis of vaporizable compounds, and in particular of polycyclic aromatic hydrocarbons which may be induced to fluoresce. The sample to be analyzed is injected into a gas chromatography column and is eluted through a narrow orifice into a vacuum chamber. The free expansion of the eluted sample into the vacuum chamber creates a supersonic molecular beam in which the sample molecules are cooled to the extent that the excited vibrational and rotational levels are substantially depopulated. The cooled molecules, when induced to fluoresce by laser excitation, give greatly simplified spectra suitable for analytical purposes. The laser induced fluorimetry provides great selectivity, and the gas chromatograph provides quantitative transfer of the sample to the molecular beam. 3 figures, 2 tables.

  10. A novel two-dimensional liquid chromatographic system for the online toxicity prediction of pharmaceuticals and related substances

    Energy Technology Data Exchange (ETDEWEB)

    Li, Jian; Xu, Li [Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan 430030 (China); Shi, Zhi-guo, E-mail: shizg@whu.edu.cn [Department of Chemistry, Wuhan University, Wuhan 430072 (China); Hu, Min [Hubei Instrument for Food and Drug Control, Wuhan (China)

    2015-08-15

    Highlights: • A novel two-dimensional liquid chromatographic system was developed. • The 1st dimension was ODS to separate components in the sample. • The 2nd dimension was biopartitioning micellar chromatography to predict toxicity. • The system was used to screen toxicity of pharmaceuticals and related substances. • It was promising for fast online toxicity screening of complex sample in one step. - Abstract: In this study, a novel two-dimensional liquid chromatographic (2D-LC) system was developed for simultaneous separation and toxicity prediction of pharmaceutical and its related substances. A conventional ODS column was used on the 1st-D to separate the sample; while, bio-partitioning micellar chromatography served as the 2nd-D to predict toxicity of the components. The established system was tested for the toxicity of ibuprofen and its impurities with known toxicity. With only one injection, ibuprofen and its impurities were separated on the 1st-D; and LC50 values of individual impurity were obtained based on the quantitative retention–activity relationships, which agreed well with the reported data. Furthermore, LC50 values of photolysis transformation products (TPs) of carprofen, ketoprofen and diclofenac acid (as unknown compounds) were screened in this 2D-LC system, which could be an indicator of the toxicity of these TPs and was meaningful for the environmental monitoring and drinking water treatment. The established 2D-LC system was cost-effective, time-saving and reliable, and was promising for fast online screening of toxicity of known and unknown analytes in the complex sample in a single step. It may find applications in environment, pharmaceutical and food, etc.

  11. A novel two-dimensional liquid chromatographic system for the online toxicity prediction of pharmaceuticals and related substances

    International Nuclear Information System (INIS)

    Li, Jian; Xu, Li; Shi, Zhi-guo; Hu, Min

    2015-01-01

    Highlights: • A novel two-dimensional liquid chromatographic system was developed. • The 1st dimension was ODS to separate components in the sample. • The 2nd dimension was biopartitioning micellar chromatography to predict toxicity. • The system was used to screen toxicity of pharmaceuticals and related substances. • It was promising for fast online toxicity screening of complex sample in one step. - Abstract: In this study, a novel two-dimensional liquid chromatographic (2D-LC) system was developed for simultaneous separation and toxicity prediction of pharmaceutical and its related substances. A conventional ODS column was used on the 1st-D to separate the sample; while, bio-partitioning micellar chromatography served as the 2nd-D to predict toxicity of the components. The established system was tested for the toxicity of ibuprofen and its impurities with known toxicity. With only one injection, ibuprofen and its impurities were separated on the 1st-D; and LC50 values of individual impurity were obtained based on the quantitative retention–activity relationships, which agreed well with the reported data. Furthermore, LC50 values of photolysis transformation products (TPs) of carprofen, ketoprofen and diclofenac acid (as unknown compounds) were screened in this 2D-LC system, which could be an indicator of the toxicity of these TPs and was meaningful for the environmental monitoring and drinking water treatment. The established 2D-LC system was cost-effective, time-saving and reliable, and was promising for fast online screening of toxicity of known and unknown analytes in the complex sample in a single step. It may find applications in environment, pharmaceutical and food, etc

  12. Automated-process gas-chromatograph system for use in accelerated corrosion testing of HTGR core-support posts

    International Nuclear Information System (INIS)

    Harper, R.E.; Herndon, P.G.

    1982-01-01

    An automated-process gas chromatograph is the heart of a gaseous-impurities-analysis system developed for the Oak Ridge National Laboratory Core Support Performance Test, at which graphite core-support posts for high-temperature gas-cooled fission reactors are being subjected to accelerated corrosion tests under tightly controlled conditions of atmosphere and temperature. Realistic estimation of in-core corrosion rates is critically dependent upon the accurate measurement of low concentrations of CO, CO 2 , CH 4 , H 2 , and O 2 in the predominantly helium atmosphere. In addition, the capital and labor investment associated with each test puts a premium upon the reliability of the analytical system, as excessive downtime or failure to obtain accurate data would result in unacceptable costs and schedule delays. After an extensive survey of available measurement techniques, gas chromatography was chosen for reasons of accuracy, flexibility, good-performance record, and cost

  13. Chromatographic separation of rhenium in alumina-methanol/sulfuric acid system

    International Nuclear Information System (INIS)

    Oguma, Koichi

    1983-01-01

    The adsorption behavior of a number of metals on alumina was surveyed in a methanol-(0.005 -- 0.5) M H 2 SO 4 (3 : 1 v/v) developing solvent by thin-layer chromatography. Over the acid concentration range tested, Re(VII) does not favor the alumina phase to any great extent while the most other metals are strongly adsorbed on alumina. These findings allowed to establish a column chromatographic technique for selective separation of rhenium in a methanol-0.05 M H 2 SO 4 (3 : 1 v/v) eluent. The separation technique thus established was applied to molybdenite analysis for rhenium. About 100-mg powdered sample containing ca. 100 ppm rhenium was decomposed with HNO 3 and then evaporated nearly to dryness. The residue was dissolved in NH 4 OH and the excess NH 4 OH was expelled by evaporation to dryness. The residue was dissolved in 2.5-ml 0.5 M H 2 SO 4 and 10-ml water, the insoluble materials filtered off, and the filtrate diluted to exactly 25 ml with water. A 10-ml aliquot of this solution was mixed with 30-ml methanol and the mixture was passed through a column (diameter 15 mm, bed height 30 mm) containing 5 g of alumina. The column was then washed with 20 ml of a methanol-0.05 M H 2 SO 4 (3 : 1 v/v) mixture. Rhenium was recovered from the loaded solution and the subsequent washings, and was determined spectrophotometrically with Methylene Blue as a chromogenic reagent. The values obtained from four samples of molybdenite are in good agreement with those obtained by neutron activation analysis. The relative standard deviation (n = 4; calculated from the range) was between 2.0 and 5.2 %. (author)

  14. Connectivity for underway Coast Guard patrol boats

    OpenAIRE

    Busch, Gregory C.

    1997-01-01

    Approved for public release; distribution is unlimited This thesis examines the US Coast Guard patrol boat's ability to effectively exchange operational data while underway. The patrol boat is currently unable to obtain tactical law enforcement information from the central Law Enforcement Information System 2 (LEIS 2) database while on patrol. LEIS 2 provides access to law enforcement information from Coast Guard, FBI, and state and local law enforcement agencies. Availability of this info...

  15. Characterizing the selectivity of stationary phases and organic modifiers in reversed-phase high-performance liquid chromatographic systems by a general solvation equation using gradient elution.

    Science.gov (United States)

    Du, C M; Valko, K; Bevan, C; Reynolds, D; Abraham, M H

    2000-11-01

    Retention data for a set of 69 compounds using rapid gradient elution are obtained on a wide range of reversed-phase stationary phases and organic modifiers. The chromatographic stationary phases studied are Inertsil (IN)-ODS, pentafluorophenyl, fluoro-octyl, n-propylcyano, Polymer (PLRP-S 100), and hexylphenyl. The organic solvent modifiers are 2,2,2-trifluoroethanol (TFE); 1,1,1,3,3,3-hexafluoropropan-2-ol (HFIP); isopropanol; methanol (MeOH); acetonitrile (AcN); tetrahydrofuran; 1,4-dioxane; N,N-dimethylformamide; and mixed solvents of dimethylsulfoxide (DMSO) with AcN and DMSO with MeOH (1:1). A total of 25 chromatographic systems are analyzed using a solvation equation. In general, most of the systems give reasonable statistics. The selectivity of the reversed phase-high-performance liquid chromatographic (HPLC) systems with respect to the solute's dipolarity-polarity, hydrogen-bond acidity, and basicity are reflected in correspondingly large coefficients in the solvation equation. We wanted to find the most orthogonal HPLC systems, showing the highest possible selectivity difference in order to derive molecular descriptors using the gradient retention times of a compound. We selected eight chromatographic systems that have a large range of coefficients of interest (s, a, and b) similar to those found in water-solvent partitions used previously to derive molecular descriptors. The systems selected are IN-ODS phases with AcN, MeOH, TFE, and HFIP as mobile phase, PLRP-S 100 phase with AcN, propylcyano phase with AcN and MeOH, and fluorooctyl phase with TFE. Using the retention data obtained for a compound in the selected chromatographic systems, we can estimate the molecular descriptors with the faster and simpler gradient elution method.

  16. Research Ship Oceanus Underway Meteorological Data, Quality Controlled

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Research Ship Oceanus Underway Meteorological Data (delayed ~10 days for quality control) are from the Shipboard Automated Meteorological and Oceanographic System...

  17. NOAA Ship Okeanos Explorer Underway Meteorological Data, Near Real Time

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NOAA Ship Okeanos Explorer Underway Meteorological Data (Near Real Time, updated daily) are from the Shipboard Automated Meteorological and Oceanographic System...

  18. Research Ship Melville Underway Meteorological Data, Quality Controlled

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Research Ship Melville Underway Meteorological Data (delayed ~10 days for quality control) are from the Shipboard Automated Meteorological and Oceanographic System...

  19. NOAA Ship Nancy Foster Underway Meteorological Data, Near Real Time

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NOAA Ship Nancy Foster Underway Meteorological Data (Near Real Time, updated daily) are from the Shipboard Automated Meteorological and Oceanographic System (SAMOS)...

  20. Research Ship Healy Underway Meteorological Data, Quality Controlled

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Research Ship Healy Underway Meteorological Data (delayed ~10 days for quality control) are from the Shipboard Automated Meteorological and Oceanographic System...

  1. Research Ship Knorr Underway Meteorological Data, Quality Controlled

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Research Ship Knorr Underway Meteorological Data (delayed ~10 days for quality control) are from the Shipboard Automated Meteorological and Oceanographic System...

  2. NOAA Ship Delaware II Underway Meteorological Data, Quality Controlled

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NOAA Ship Delaware II Underway Meteorological Data (delayed ~10 days for quality control) are from the Shipboard Automated Meteorological and Oceanographic System...

  3. Research Ship Atlantis Underway Meteorological Data, Quality Controlled

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Research Ship Atlantis Underway Meteorological Data (delayed ~10 days for quality control) are from the Shipboard Automated Meteorological and Oceanographic System...

  4. NOAA Ship Pisces Underway Meteorological Data, Quality Controlled

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NOAA Ship Pisces Underway Meteorological Data (delayed ~10 days for quality control) are from the Shipboard Automated Meteorological and Oceanographic System (SAMOS)...

  5. NOAA Ship Fairweather Underway Meteorological Data, Quality Controlled

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NOAA Ship Fairweather Underway Meteorological Data (delayed ~10 days for quality control) are from the Shipboard Automated Meteorological and Oceanographic System...

  6. NOAA Ship Hi'ialakai Underway Meteorological Data, Quality Controlled

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NOAA Ship Hi'ialakai Underway Meteorological Data (delayed ~10 days for quality control) are from the Shipboard Automated Meteorological and Oceanographic System...

  7. NOAA Ship Ronald Brown Underway Meteorological Data, Quality Controlled

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NOAA Ship Ronald Brown Underway Meteorological Data (delayed ~10 days for quality control) are from the Shipboard Automated Meteorological and Oceanographic System...

  8. NOAA Ship Miller Freeman Underway Meteorological Data, Quality Controlled

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NOAA Ship Miller Freeman Underway Meteorological Data (delayed ~10 days for quality control) are from the Shipboard Automated Meteorological and Oceanographic System...

  9. NOAA Ship Gordon Gunter Underway Meteorological Data, Near Real Time

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NOAA Ship Gordon Gunter Underway Meteorological Data (Near Real Time, updated daily) are from the Shipboard Automated Meteorological and Oceanographic System (SAMOS)...

  10. NOAA Ship Oregon II Underway Meteorological Data, Near Real Time

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NOAA Ship Oregon II Underway Meteorological Data (Near Real Time, updated daily) are from the Shipboard Automated Meteorological and Oceanographic System (SAMOS)...

  11. NOAA Ship Oscar Dyson Underway Meteorological Data, Quality Controlled

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NOAA Ship Oscar Dyson Underway Meteorological Data (delayed ~10 days for quality control) are from the Shipboard Automated Meteorological and Oceanographic System...

  12. NOAA Ship Fairweather Underway Meteorological Data, Near Real Time

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NOAA Ship Fairweather Underway Meteorological Data (Near Real Time, updated daily) are from the Shipboard Automated Meteorological and Oceanographic System (SAMOS)...

  13. Research Ship Kilo Moana Underway Meteorological Data, Quality Controlled

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Research Ship Kilo Moana Underway Meteorological Data (delayed ~10 days for quality control) are from the Shipboard Automated Meteorological and Oceanographic System...

  14. NOAA Ship Nancy Foster Underway Meteorological Data, Quality Controlled

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NOAA Ship Nancy Foster Underway Meteorological Data (delayed ~10 days for quality control) are from the Shipboard Automated Meteorological and Oceanographic System...

  15. NOAA Ship Gordon Gunter Underway Meteorological Data, Quality Controlled

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NOAA Ship Gordon Gunter Underway Meteorological Data (delayed ~10 days for quality control) are from the Shipboard Automated Meteorological and Oceanographic System...

  16. NOAA Ship Rainier Underway Meteorological Data, Quality Controlled

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NOAA Ship Rainier Underway Meteorological Data (delayed ~10 days for quality control) are from the Shipboard Automated Meteorological and Oceanographic System...

  17. Research Ship Tangaroa Underway Meteorological Data, Quality Controlled

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Research Ship Tangaroa Underway Meteorological Data (delayed ~10 days for quality control) are from the Shipboard Automated Meteorological and Oceanographic System...

  18. NOAA Ship Ka'imimoana Underway Meteorological Data, Quality Controlled

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NOAA Ship Ka'imimoana Underway Meteorological Data (delayed ~10 days for quality control) are from the Shipboard Automated Meteorological and Oceanographic System...

  19. NOAA Ship Oregon II Underway Meteorological Data, Quality Controlled

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NOAA Ship Oregon II Underway Meteorological Data (delayed ~10 days for quality control) are from the Shipboard Automated Meteorological and Oceanographic System...

  20. NOAA Ship Rainier Underway Meteorological Data, Near Real Time

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NOAA Ship Rainier Underway Meteorological Data (Near Real Time, updated daily) are from the Shipboard Automated Meteorological and Oceanographic System (SAMOS)...

  1. NOAA Ship Pisces Underway Meteorological Data, Near Real Time

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NOAA Ship Pisces Underway Meteorological Data (Near Real Time, updated daily) are from the Shipboard Automated Meteorological and Oceanographic System (SAMOS)...

  2. NOAA Ship Ronald Brown Underway Meteorological Data, Near Real Time

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NOAA Ship Ronald Brown Underway Meteorological Data (Near Real Time, updated daily) are from the Shipboard Automated Meteorological and Oceanographic System (SAMOS)...

  3. NOAA Ship Oscar Dyson Underway Meteorological Data, Near Real Time

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NOAA Ship Oscar Dyson Underway Meteorological Data (Near Real Time, updated daily) are from the Shipboard Automated Meteorological and Oceanographic System (SAMOS)...

  4. Automated chromatographic system with polarimetric detection laser applied in the control of fermentation processes and seaweed extracts characterization

    International Nuclear Information System (INIS)

    Fajer, V.; Naranjo, S.; Mora, W.; Patinno, R.; Coba, E.; Michelena, G.

    2012-01-01

    There are presented applications and innovations of chromatographic and polarimetric systems in which develop methodologies for measuring the input molasses and the resulting product of a fermentation process of alcohol from a rich honey and evaluation of the fermentation process honey servery in obtaining a drink native to the Yucatan region. Composition was assessed optically active substances in seaweed, of interest to the pharmaceutical industry. The findings provide measurements alternative raw materials and products of the sugar industry, beekeeping and pharmaceutical liquid chromatography with automated polarimetric detection reduces measurement times up to 15 min, making it comparable to the times of high chromatography resolution, significantly reducing operating costs. By chromatography system with polarimetric detection (SCDP) is new columns have included standard size designed by the authors, which allow process samples with volumes up to 1 ml and reduce measurement time to 15 min, decreasing to 5 times the volume sample and halving the time of measurement. Was evaluated determining the concentration of substances using the peaks of the chromatograms obtained for the different columns and calculate the uncertainty of measurements. The results relating to the improvement of a data acquisition program (ADQUIPOL v.2.0) and new programs for the preparation of chromatograms (CROMAPOL CROMAPOL V.1.0 and V.1.2) provide important benefits, which allow a considerable saving of time the processing of the results and can be applied in other chromatography systems with the appropriate adjustments. (Author)

  5. A low-power pressure-and temperature-programmed separation system for a micro gas chromatograph.

    Energy Technology Data Exchange (ETDEWEB)

    Sacks, Richard D. (University of Michigan, Ann Arbor, MI); Robinson, Alex Lockwood (Advanced Sensor Technologies, Albuquerque, NM); Lambertus, Gordon R. (University of Michigan, Ann Arbor, MI); Potkay, Joseph A. (University of Michigan, Ann Arbor, MI); Wise, Kensall D. (University of Michigan, Ann Arbor, MI)

    2006-10-01

    This thesis presents the theory, design, fabrication and testing of the microvalves and columns necessary in a pressure- and temperature-programmed micro gas chromatograph ({micro}GC). Two microcolumn designs are investigated: a bonded Si-glass column having a rectangular cross section and a vapor-deposited silicon oxynitride (Sion) column having a roughly circular cross section. Both microcolumns contain integrated heaters and sensors for rapid, controlled heating. The 3.2 cm x 3.2 cm, 3 m-long silicon-glass column, coated with a non-polar polydimethylsiloxane (PDMS) stationary phase, separates 30 volatile organic compounds (VOCs) in less than 6 min. This is the most efficient micromachined column reported to date, producing greater than 4000 plates/m. The 2.7 mm x 1.4 mm Sion column eliminates the glass sealing plate and silicon substrate using deposited dielectrics and is the lowest power and fastest GC column reported to date; it requires only 11 mW to raise the column temperature by 100 C and has a response time of 11s and natural temperature ramp rate of 580 C/min. A 1 m-long PDMS-coated Sion microcolumn separates 10 VOCs in 52s. A system-based design approach was used for both columns.

  6. Chemiluminescent Determination of Oxamyl in Drinking Water and Tomato Using Online Postcolumn UV Irradiation in a Chromatographic System.

    Science.gov (United States)

    Murillo Pulgarín, José A; García Bermejo, Luisa F; Durán, Armando Carrasquero

    2018-03-07

    High-performance liquid chromatography (HPLC) was used to separate oxamyl from other pesticides in drinking water and tomato paste. The eluate emerging from the column tail was mixed with an alkaline solution of Co 2+ in EDTA and irradiated with UV light to induce photolysis of the carbamate in order to obtain free radicals and other reactive species that oxidize luminol and produce chemiluminescence (CL) as a result. The intensity of the CL signal was monitored in the form of chromatographic peaks. Under the optimum operating conditions for the HPLC-UV-CL system, the analyte concentration was linearly related to peak area. The limit of detection as determined in accordance with the IUPAC criterion was 0.17 mg L -1 . Oxamyl was successfully extracted with recoveries of 88.7-103.1% from spiked tomato paste by using a simple QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) sample preparation approach. Similar recoveries were obtained from drinking water samples spiked with oxamyl concentrations above the LOD. The proposed method is a simple, fast, accurate choice for quantifying this pesticide.

  7. Improved Chromatographic Bioavailability Estimations

    National Research Council Canada - National Science Library

    Dorsey, John

    1996-01-01

    .... Since the inception of reversed phase liquid chromatography there have been many attempts to correlate chromatographic retention with bioavailability and the most often used bulk measure, the octanol...

  8. Chromatographic study of element 104 in the system HCl/triisobutylphosphate (TBP)

    Energy Technology Data Exchange (ETDEWEB)

    Guenther, R.; Paulus, W.; Kratz, J.V.; Seibert, A.; Thoerle, P.; Zauner, S. [Mainz Univ. (Germany); Bruechle, W.; Jaeger, E.; Schaedel, M.; Schausten, B. [Gesellschaft fuer Schwerionenforschung mbH, Darmstadt (Germany); Schumann, D. [Technische Univ. Dresden (Germany); Eichler, B.; Jost, D.T.; Tuerler, A.; Gaeggeler, H.W. [Paul Scherrer Inst. (PSI), Villigen (Switzerland)

    1997-09-01

    With the Automated Rapid Chemistry Apparatus ARCA chromatic experiments have been performed using the chemical system hydrochloric acid/tributylphosphate with element 104 (Rf). The results indicate that Rf behaves more like its homolog Hf. (author) 2 figs., 5 refs.

  9. Development of an on-line size exclusion chromatographic - reversed-phase liquid chromatographic two-dimensional system for the quantitative determination of peptides with concentration prior to reversed-phase liquid chromatographic separation

    NARCIS (Netherlands)

    Stroink, T.; Wiese, G.; Lingeman, H.; Bult, A.; Underberg, W.J.M.

    2001-01-01

    Complex samples, containing endogenous peptides require analytical methods with high sensitivity and selectivity to enable reliable analysis of these compounds in biological samples. In a number of cases, the selectivity of mono-dimensional separation systems is not sufficient. In order to improve

  10. Alternative chromatographic system for the quality control of lipophilic technetium-99m radiopharmaceuticals such as [{sup 99m}Tc(MIBI){sub 6}]{sup +}

    Energy Technology Data Exchange (ETDEWEB)

    Faria, D.P.; Buchpiguel, C.A.; Marques, F.L.N., E-mail: danielefaria1@gmail.com [Universidade de Sao Paulo (USP), Sao Paulo, SP (Brazil). Faculdade de Medicina. Departamento de Radiologia. Servico de Medicina Nuclear

    2015-10-15

    Knowledge of the radiochemical purity of radiopharmaceuticals is mandatory and can be evaluated by several methods and techniques. Planar chromatography is the technique normally employed in nuclear medicine since it is simple, rapid and usually of low cost. There is no standard system for the chromatographic technique, but price, separation efficiency and short time for execution must be considered. We have studied an alternative system using common chromatographic stationary phase and alcohol or alcohol:chloroform mixtures as the mobile phase, using the lipophilic radiopharmaceutical [{sup 99m}Tc(MIBI){sub 6}]{sup +} as a model. Whatman 1 modified phase paper and absolute ethanol, Whatman 1 paper and methanol: chloroform (25:75), Whatman 3MM paper and ethanol:chloroform (25:75), and the more expensive ITLC-SG and 1-propanol: chloroform (10:90) were suitable systems for the direct determination of radiochemical purity of [{sup 99m}Tc(MIBI){sub 6}]{sup +} since impurities such as {sup 99m}Tc-reduced-hydrolyzed (RH), {sup 99m}TcO4{sup -} and [{sup 99m}Tc(cysteine){sub 2}]{sup -} complex were completely separated from the radiopharmaceutical, which moved toward the front of chromatographic systems while impurities were retained at the origin. The time required for analysis was 4 to 15 min, which is appropriate for nuclear medicine routines. (author)

  11. Alternative chromatographic system for the quality control of lipophilic technetium-99m radiopharmaceuticals such as [99mTc(MIBI6]+

    Directory of Open Access Journals (Sweden)

    D.P. Faria

    2015-01-01

    Full Text Available Knowledge of the radiochemical purity of radiopharmaceuticals is mandatory and can be evaluated by several methods and techniques. Planar chromatography is the technique normally employed in nuclear medicine since it is simple, rapid and usually of low cost. There is no standard system for the chromatographic technique, but price, separation efficiency and short time for execution must be considered. We have studied an alternative system using common chromatographic stationary phase and alcohol or alcohol:chloroform mixtures as the mobile phase, using the lipophilic radiopharmaceutical [99mTc(MIBI6]+ as a model. Whatman 1 modified phase paper and absolute ethanol, Whatman 1 paper and methanol:chloroform (25:75, Whatman 3MM paper and ethanol:chloroform (25:75, and the more expensive ITLC-SG and 1-propanol:chloroform (10:90 were suitable systems for the direct determination of radiochemical purity of [99mTc(MIBI6]+ since impurities such as 99mTc-reduced-hydrolyzed (RH, 99mTcO4 - and [99mTc(cysteine2]- complex were completely separated from the radiopharmaceutical, which moved toward the front of chromatographic systems while impurities were retained at the origin. The time required for analysis was 4 to 15 min, which is appropriate for nuclear medicine routines.

  12. Retention behavior of selected alkaloids in Reversed Phase micellar chromatographic systems

    Directory of Open Access Journals (Sweden)

    Petruczynik Anna

    2015-06-01

    Full Text Available In this work, the effects of sodium dodecyl sulfate (SDS concentrations on retention, separation selectivity, peak shapes and systems efficiency were investigated. Herein, the retention data for 11 alkaloids were determined on an RP18 silica column with mobile phases containing methanol as organic modifier, with acetate buffer at pH 3.5, and, subsequently, with the addition of sodium dodecyl sulfate (SDS. The results of this study indicate that the retention of alkaloids decreases with the increase of SDS concentration in the mobile phase. The increase of SDS concentration, however, leads to the significantly improvement of peak symmetry and the increase of theoretical plate number in all cases. The best system efficiency for most of the investigated alkaloids was obtained in a mobile phase containing 0.1 M SDS, while most symmetrical peaks were obtained through the addition of 0.3 M of SDS to the mobile phase.

  13. System for separation of cortisol, 11-deoxycortisol, 17-hydroxyprogesterone and progesterone in a single chromatographic step and its application to radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Cunningham, S K; McKenna, T J [St. Vincent' s Hospital, Dublin (Republic of Ireland). Dept. of Endocrinology

    1982-08-18

    A procedure for accurate, sensitive and highly specific measurement of cortisol, 11-deoxycortisol, 17-hydroxyprogesterone and progesterone is described. We have developed a chromatographic system whereby the steroids may be separated in a single passage over celite microcolumns. This permits the specific radioimmunoassay of the principal glucocorticoid in man and its ..delta..4-pregnene precursors and thus facilitates investigation of the physiological control and pathological disorders of glucocorticoid biosynthesis.

  14. Development of a chromatographic micro-system for radionuclides analysis in nitric acid media

    International Nuclear Information System (INIS)

    Losno, Marion

    2017-01-01

    Radionuclides analysis is a key point for nuclear waste management and nuclear material control. Several steps of sample modification have to be carried out before measurements in order to avoid any interferences and improve measurement precision. However those different steps are long, irradiating and difficult to achieve in gloveboxes. Moreover they produce liquid and solid waste. The goal of the study is to offer a new alternative to the use of solid phase extraction column for radionuclides separation in hard nitric acid medium. The system will decrease the amount of nuclear waste due to the analysis and automatize the different steps of the analysis. A plastic device made of COC containing a micro solid phase extraction column is first designed. Stationary phase is a poly(AMA-co-EDMA) monolith synthesized in situ. Its structure is adjustable and its functionalization versatile with a high resistance to nitric acid medium. Exchange capacity is 150 mg/g of monolith for TBP and TBP/CMPO column and up to 280 mg/g of monolith in case of DAAP. Exchange coefficients are determined for U(VI), Th(IV), Eu(III) and Nd(III) for 3 different extractants (and Pu(IV) in case of TBP column). Monolith synthesis is transferred in centrifugal device and hydrodynamic behavior studied. U,Th/Eu separation was finally carried out in both classic and centrifugal micro-system on TBP column. (author) [fr

  15. Water quality monitoring of Jialing-River in Chongqing using advanced ion chromatographic system.

    Science.gov (United States)

    Tanaka, Kazuhiko; Shi, Chao-Hong; Nakagoshi, Nobukazu

    2012-04-01

    The water quality monitoring operation to evaluate the water quality of polluted river is an extremely important task for the river-watershed management/control based on the environmental policy. In this study, the novel, simple and convenient water quality monitoring of Jialing-River in Chongqing, China was carried out using an advanced ion chromatography (IC) consisting of ion-exclusion/cation-exchange chromatography (IEC/CEC) with conductivity detection for determining simultaneously the common anions such as SO4(2-), Cl(-), and NO3(-) and the cations such as Na+, NH4+, K+, Mg2+, and Ca2+, the ion-exclusion chromatography (IEC) with visible detection for determining simultaneously the nutrient components such as phosphate and silicate ions, and the IEC with the enhanced conductivity detection using a post column of K+-form cation-exchange resin for determining HCO3(-)-alkalinity as an inorganic-carbon source for biomass synthesis in biological reaction process under the aerobic conditions. According to the ionic balance theory between the total equivalent concentrations of anions and cations, the water quality evaluation of the Jialing-River waters taking at different sampling sites in Chongqing metropolitan area was carried out using the advanced IC system. As a result, the effectiveness of this novel water quality monitoring methodology using the IC system was demonstrated on the several practical applications to a typical biological sewage treatment plant on Jialing-River of Chongqing.

  16. A mobile automatic gas chromatograph system to measure CO2, CH4 and N2O fluxes from soil in the field

    International Nuclear Information System (INIS)

    Silvola, J.; Martikainen, P.; Nykaenen, H.

    1992-01-01

    A caravan has been converted into mobile laboratory for measuring fluxes of CO 2 , CH 4 and N 2 O from the soil in the field. The caravan was equipped with a gas chromatograph fitted with TC-, FI- and EC-detectors, and a PC controlled data logger. The gas collecting chambers can be used up to 50 m from the caravan. The closing and opening of the chambers, as well as the flows of sample gases from chambers to the gas chromatograph. is pneumatically regulated. Simultaneous recordings of temperature, light intensity and the depth of water table are made. The system has been used for two months in 1992, and some preliminary results are presented

  17. New chromatographic systems for the quality control determination of 99Tcm-tetrofosmin

    International Nuclear Information System (INIS)

    Penglis, S.; Tsopelas, C.

    1999-01-01

    Full text: The manufacturer's method for assessing the radiochemical purity (RCP) of 99 Tc m -tetrofosmin requires the use of a volatile solvent system on silica gel impregnated thin layer chromatography strips, and is a time-consuming procedure (> 15 minutes). A rapid quality control procedure for this product has already been proposed which utilizes miniature Whatman 1 paper strips with ethyl acetate as the mobile phase, where 99 Tc m -tetrofosmin appears at the solvent front (R f 0.9). In our laboratory, this procedure was found to be unreliable, prompting us to develop two new and improved methods to successfully determine the % RCP of 99 Tc m -tetrofosmin. These include: (1) an Amprep anion exchange cartridge with absolute ethanol as the mobile phase; and (2) a miniature Whatman 17 strip with ethyl acetate:acetone [1:9] as the mobile phase. 99 Tc m -tetrofosmin elutes with the solvent, and 99 Tc m O 2 plus 99 Tc m O 4 -remain on the cartridge for the cartridge method. 99 Tc m -tetrofosmin moves with the solvent front (Rf 0.8) and the other 99 Tc m -impurities remain at the origin for the Whatman paper method. The % RCP of 99 Tc m -tetrofosmin (n = 8) by the manufacturer's method was 96.5 ± 1.5%, as compared to the cartridge method (98.0 ± 0.9%) and Whatman paper method (97.3 ± 0.9%) at 0-2 h post-reconstitution. The quality control determinations gave a result in 3 or 5 min for the cartridge or Whatman paper methods respectively. These methods can be used successfully in a dose calibrator for counting, particularly if the total counts are > 1.5 MBq. These new methods were found to be highly reproducible, convenient and rapid for the routine analysis of 99 Tc m -tetrofosmin preparations

  18. Technical Note: Precise quantitative measurements of total dissolved inorganic carbon from small amounts of seawater using a gas chromatographic system

    Directory of Open Access Journals (Sweden)

    T. Hansen

    2013-10-01

    Full Text Available Total dissolved inorganic carbon (CT is one of the most frequently measured parameters used to calculate the partial pressure of carbon dioxide in seawater. Its determination has become increasingly important because of the rising interest in the biological effects of ocean acidification. Coulometric and infrared detection methods are currently favored in order to precisely quantify CT. These methods however are not sufficiently validated for CT measurements of biological experiments manipulating seawater carbonate chemistry with an extended CT measurement range (~1250–2400 μmol kg–1 compared to natural open ocean seawater (~1950–2200 μmol kg−1. The requirement of total sample amounts between 0.1–1 L seawater in the coulometric- and infrared detection methods potentially exclude their use for experiments working with much smaller volumes. Additionally, precise CT analytics become difficult with high amounts of biomass (e.g., phytoplankton cultures or even impossible in the presence of planktonic calcifiers without sample pre-filtration. Filtration however, can alter CT concentration through gas exchange induced by high pressure. Addressing these problems, we present precise quantification of CT using a small, basic and inexpensive gas chromatograph as a CT analyzer. Our technique is able to provide a repeatability of ±3.1 μmol kg−1, given by the pooled standard deviation over a CT range typically applied in acidification experiments. 200 μL of sample is required to perform the actual CT measurement. The total sample amount needed is 12 mL. Moreover, we show that sample filtration is applicable with only minor alteration of the CT. The method is simple, reliable and with low cumulative material costs. Hence, it is potentially attractive for all researchers experimentally manipulating the seawater carbonate system.

  19. A gas chromatograph system for semi-continuous greenhouse gas measurements at Puy de Dôme station, Central France

    Science.gov (United States)

    Lopez, M.; Schmidt, M.; Ramonet, M.; Bonne, J.-L.; Colomb, A.; Kazan, V.; Laj, P.; Pichon, J.-M.

    2015-03-01

    Three years of greenhouse gases measurements, obtained using a gas chromatograph (GC) system located at the Puy de Dôme station at 1465 m a.s.l. in Central France are presented. The GC system was installed in 2010 at Puy de Dôme and was designed for automatic and accurate semi-continuous measurements of atmospheric carbon dioxide, methane, nitrous oxide and sulfur hexafluoride mole fractions. We present in detail the instrumental set up and the calibration strategy, which together allow the GC to reach repeatabilities of 0.1 μmol mol-1, 1.2, 0.3 nmol mol-1 and 0.06 pmol mol-1 for CO2, CH4, N2O and SF6, respectively. Comparisons of the atmospheric time series with those obtained using other instruments shown that the GC system meets the World Meteorological Organization recommendations. The analysis of the three-year atmospheric time series revealed how the planetary boundary layer height drives the mole fractions observed at a mountain site such as Puy de Dôme where air masses alternate between the planetary boundary layer and the free troposphere. Accurate long-lived greenhouse gases measurements collocated with 222Rn measurements as an atmospheric tracer, allowed us to determine the CO2, CH4 and N2O emissions in the catchment area of the station. The derived CO2 surface flux revealed a clear seasonal cycle with net uptake by plant assimilation in the spring and net emission caused by the biosphere and burning of fossil fuel during the remainder of the year. We calculated a mean annual CO2 flux of 1150 t(CO2) km-2. The derived CH4 and N2O emissions in the station catchment area were 5.6 t(CH4) km-2 yr-1 and 1.5 t(N2O) km-2 yr-1, respectively. Our derived annual CH4 flux is in agreement with the national French inventory, whereas our derived N2O flux is five times larger than the same inventory.

  20. Chromatographic separations of stereoisomers

    Energy Technology Data Exchange (ETDEWEB)

    Souter, R.W.

    1985-01-01

    This text covers both diastereomers and enantiomers; describes techniques for GC, HPLC, and other chromatographic methods; and tabulates results of various applications by both techniques and compound class. It provides current knowledge about separation mechanisms and interactions of asymmetric molecules, as well as experimental and commercial materials such as columns, instruments, and derivatization reagents. The contents also include stereoisomer separations by gas chromatography. Stereoisomer separations by high-performance liquid chromatography. Stereoisomer separations by other chromatographic techniques.

  1. Chromatographic hydrogen isotope separation

    International Nuclear Information System (INIS)

    Aldridge, F.T.

    1983-01-01

    Intermetallic compounds with the CaCu5 type of crystal structure, particularly LaNiCo and CaNi5, exhibit high separation factors and fast equilibrium times and therefore are useful for packing a chromatographic hydrogen isotope separation column. The addition of an inert metal to dilute the hydride improves performance of the column. A large scale multi-stage chromatographic separation process run as a secondary process off a hydrogen feedstream from an industrial plant which uses large volumes of hydrogen can produce large quantities of heavy water at an effective cost for use in heavy water reactors

  2. Chromatographic hydrogen isotope separation

    International Nuclear Information System (INIS)

    Aldridge, F.T.

    1981-01-01

    Intermetallic compounds with the CaCu5 type of crystal structure , particularly LaNiCo and CaNi5, exhibit high separation factors and fast equilibrium times and therefore are useful for packing a chromatographic hydrogen isotope separation colum. The addition of an inert metal to dilute the hydride improves performance of the column. A large scale mutli-stage chromatographic separation process run as a secondary process off a hydrogen feedstream from an industrial plant which uses large volumes of hydrogen can produce large quantities of heavy water at an effective cost for use in heavy water reactors

  3. Vibrational Spectroscopy of Chromatographic Interfaces

    Energy Technology Data Exchange (ETDEWEB)

    Jeanne E. Pemberton

    2011-03-10

    Chromatographic separations play a central role in DOE-supported fundamental research related to energy, biological systems, the environment, and nuclear science. The overall portfolio of research activities in the Separations and Analysis Program within the DOE Office of Basic Energy Sciences includes support for activities designed to develop a molecular-level understanding of the chemical processes that underlie separations for both large-scale and analytical-scale purposes. The research effort funded by this grant award was a continuation of DOE-supported research to develop vibrational spectroscopic methods to characterize the interfacial details of separations processes at a molecular level.

  4. Domestic Preparedness Program: Evaluation of the Agilent Gas Chromatograph - Flame Photometric Detector/Mass Selective Detector (GC-FPD/MSD) System Against Chemical Warfare Agents Summary Report

    National Research Council Canada - National Science Library

    Longworth, Terri

    2003-01-01

    This report characterizes the chemical warfare agent (CWA) detection potential of the commercially available Agilent gas chromatograph-flame photometric detector/mass selective detector (GC-FPD/MSD...

  5. NOAA Ship Ka'imimoana Underway Meteorological Data, Near Real Time

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NOAA Ship Ka'imimoana Underway Meteorological Data (Near Real Time, updated daily) are from the Shipboard Automated Meteorological and Oceanographic System (SAMOS)...

  6. NOAA Ship Bell M. Shimada Underway Meteorological Data, Near Real Time

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NOAA Ship Bell M. Shimada Underway Meteorological Data (Near Real Time, updated daily) are from the Shipboard Automated Meteorological and Oceanographic System...

  7. NOAA Ship Henry B. Bigelow Underway Meteorological Data, Near Real Time

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NOAA Ship Henry B. Bigelow Underway Meteorological Data (Near Real Time, updated daily) are from the Shipboard Automated Meteorological and Oceanographic System...

  8. NOAA Ship McArthurII Underway Meteorological Data, Quality Controlled

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NOAA Ship McArthur II Underway Meteorological Data (delayed ~10 days for quality control) are from the Shipboard Automated Meteorological and Oceanographic System...

  9. NOAA Ship Oscar Elton Sette Underway Meteorological Data, Near Real Time

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NOAA Ship Oscar Elton Sette Underway Meteorological Data (Near Real Time, updated daily) are from the Shipboard Automated Meteorological and Oceanographic System...

  10. NOAA Ship Hi'ialakai Underway Meteorological Data, Near Real Time

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NOAA Ship Hi'ialakai Underway Meteorological Data (Near Real Time, updated daily) are from the Shipboard Automated Meteorological and Oceanographic System (SAMOS)...

  11. Incorporating high-pressure electroosmotic pump and a nano-flow gradient generator into a miniaturized liquid chromatographic system for peptide analysis.

    Science.gov (United States)

    Chen, Apeng; Lynch, Kyle B; Wang, Xiaochun; Lu, Joann J; Gu, Congying; Liu, Shaorong

    2014-09-24

    We integrate a high-pressure electroosmotic pump (EOP), a nanoflow gradient generator, and a capillary column into a miniaturized liquid chromatographic system that can be directly coupled with a mass spectrometer for proteomic analysis. We have recently developed a low-cost high-pressure EOP capable of generating pressure of tens of thousands psi, ideal for uses in miniaturized HPLC. The pump worked smoothly when it was used for isocratic elutions. When it was used for gradient elutions, generating reproducible gradient profiles was challenging; because the pump rate fluctuated when the pump was used to pump high-content organic solvents. This presents an issue for separating proteins/peptides since high-content organic solvents are often utilized. In this work, we solve this problem by incorporating our high-pressure EOP with a nano-flow gradient generator so that the EOP needs only to pump an aqueous solution. With this combination, we develop a capillary-based nano-HPLC system capable of performing nano-flow gradient elution; the pump rate is stable, and the gradient profiles are reproducible and can be conveniently tuned. To demonstrate its utility, we couple it with either a UV absorbance detector or a mass spectrometer for peptide separations. Copyright © 2014. Published by Elsevier B.V.

  12. Proposal for data acquisition system of gas chromatograph and natural gas transfer custody via web; Proposta para um sistema de aquisicao de dados de cromatografia e medicao fiscal de gas natural via web

    Energy Technology Data Exchange (ETDEWEB)

    Santana, Jose Paulo C.; Guimaraes, Marcelo F.; Zeitoune, Rafael J. [PETROBRAS, Rio de Janeiro, RJ (Brazil)

    2005-07-01

    In this paper, is presented a proposal of a Chromatograph and Transfer Custody Measurement Data Acquisition System through Web, complementary to the SCADA System, responsible for control and monitoring PETROBRAS Gas Pipelines, intended to comply with the requirements of the Gerencias de Qualidade e Medicao (MQD) and Planejamento Integrado da Logistica (PCL) from PETROBRAS Gas e Energia, regarding the evaluation of the quality of the natural gas that is being commercialized, as well as its billing. (author)

  13. Study on the effect of gamma radiolysis on the chromatographic composition profile of tri-isoAmyl-phosphate-n-dodecane-nitric acid system

    International Nuclear Information System (INIS)

    Chaudhary, S.D.; Lokhande, Manisha; Bindu, M.; Tripathi, S.C.; Gandhi, P.M.

    2013-01-01

    Tri-Iso-Amyl phosphate (TiAP), a higher homologue of tri-butyl phosphate (TBP) is the solvent proposed for the reprocessing of the spent nuclear fuel of Fast Breeder Reactors (FBR) origin. TiAP-n-dodecane has extraction behavior comparable to that of TBP-n-dodecane system, without third phase formation and an aqueous solubility ∼ 19mg/L, that is far too less in case as compared to that with TBP(∼400gm/L). We have initiated our study to examine the radiolytic degradation behavior of TiAP-n-dodecane in comparison to that of TBP and explore its full potential for reprocessing of spent nuclear fuels. A 30% mixture of TiAP with n-dodecane equilibrated with nitric acid of concentrations 2M has been subjected to steady state gamma radiolysis using 60 Co source. The samples were irradiated for increasing absorbed dose of 6,12 and 18M rad. Each of these samples were subjected to gas chromatographic analysis under optimised condition using thermal conductivity detector. Separately, these samples were also methylated using diazomethane for the determination of acidic phosphate species as their volatile methyl ester

  14. Amalgam-chromatographic separation of magnesium isotopes

    International Nuclear Information System (INIS)

    Klinskij, G.D.; Levkin, A.V.; Ivanov, S.A.

    1990-01-01

    Separation of magnesium isotopes within Mg(Hg)-MgI 2 system (in dimethylformamide) is conducted under amalgam-chromatographic conditions. Separation maximal degree, that is (1.09), for 24 Mg and 26 Mg and separation coefficient (α = 1.0089±0.006) are determined. Light isotopes are found to concentrate in the amalgam. Technique of thermal conversion of flows within amalgam-dimethylformamide system is suggested on the basis of reversible reaction of Ca-Mg element exchange

  15. Loop system for creating jet fuel vapor standards used in the calibration of infrared spectrophotometers and gas chromatographs.

    Science.gov (United States)

    Reboulet, James; Cunningham, Robert; Gunasekar, Palur G; Chapman, Gail D; Stevens, Sean C

    2009-02-01

    A whole body inhalation study of mixed jet fuel vapor and its aerosol necessitated the development of a method for preparing vapor only standards from the neat fuel. Jet fuel is a complex mixture of components which partitions between aerosol and vapor when aspirated based on relative volatility of the individual compounds. A method was desired which could separate the vapor portion from the aerosol component to prepare standards for the calibration of infrared spectrophotometers and a head space gas chromatography system. A re-circulating loop system was developed which provided vapor only standards whose composition matched those seen in an exposure system. Comparisons of nominal concentrations in the exposure system to those determined by infrared spectrophotometry were in 92-95% agreement. Comparison of jet fuel vapor concentrations determined by infrared spectrophotometry compared to head space gas chromatography yielded a 93% overall agreement in trial runs. These levels of agreement show the loop system to be a viable method for creating jet fuel vapor standards for calibrating instruments.

  16. Validation for chromatographic and electrophoretic methods

    OpenAIRE

    Ribani, Marcelo; Bottoli, Carla Beatriz Grespan; Collins, Carol H.; Jardim, Isabel Cristina Sales Fontes; Melo, Lúcio Flávio Costa

    2004-01-01

    The validation of an analytical method is fundamental to implementing a quality control system in any analytical laboratory. As the separation techniques, GC, HPLC and CE, are often the principal tools used in such determinations, procedure validation is a necessity. The objective of this review is to describe the main aspects of validation in chromatographic and electrophoretic analysis, showing, in a general way, the similarities and differences between the guidelines established by the dif...

  17. Chromatographic study of nucleosides and nucleotides of central nervous system. Identification and first results on changes after total gamma irradiation of brain

    International Nuclear Information System (INIS)

    Valle, C.; Marquer, C.; Pasquier, C.

    Changes of brain energetic state and of different levels after irradiation are studied. The results will be compared with the variations of brain electric activity due to irradiation. Using an ion exchange chromatographic method for separation and quantitative analysis of nucleotides, evaluation of adenylic nucleotides in brain rat have been chosen [fr

  18. Parameter selection for peak alignment in chromatographic sample profiling: Objective quality indicators and use of control samples

    NARCIS (Netherlands)

    Peters, S.; van Velzen, E.; Janssen, H.-G.

    2009-01-01

    In chromatographic profiling applications, peak alignment is often essential as most chromatographic systems exhibit small peak shifts over time. When using currently available alignment algorithms, there are several parameters that determine the outcome of the alignment process. Selecting the

  19. An aircraft gas chromatograph-mass spectrometer System for Organic Fast Identification Analysis (SOFIA): design, performance and a case study of Asian monsoon pollution outflow

    Science.gov (United States)

    Bourtsoukidis, Efstratios; Helleis, Frank; Tomsche, Laura; Fischer, Horst; Hofmann, Rolf; Lelieveld, Jos; Williams, Jonathan

    2017-12-01

    Volatile organic compounds (VOCs) are important for global air quality and oxidation processes in the troposphere. In addition to ground-based measurements, the chemical evolution of such species during transport can be studied by performing in situ airborne measurements. Generally, aircraft instrumentation needs to be sensitive, robust and sample at higher frequency than ground-based systems while their construction must comply with rigorous mechanical and electrical safety standards. Here, we present a new System for Organic Fast Identification Analysis (SOFIA), which is a custom-built fast gas chromatography-mass spectrometry (GC-MS) system with a time resolution of 2-3 min and the ability to quantify atmospheric mixing ratios of halocarbons (e.g. chloromethanes), hydrocarbons (e.g isoprene), oxygenated VOCs (acetone, propanal, butanone) and aromatics (e.g. benzene, toluene) from sub-ppt to ppb levels. The relatively high time resolution is the result of a novel cryogenic pre-concentration unit which rapidly cools (˜ 6 °C s-1) the sample enrichment traps to -140 °C, and a new chromatographic oven designed for rapid cooling rates (˜ 30 °C s-1) and subsequent thermal stabilization. SOFIA was installed in the High Altitude and Long Range Research Aircraft (HALO) for the Oxidation Mechanism Observations (OMO) campaign in August 2015, aimed at investigating the Asian monsoon pollution outflow in the tropical upper troposphere. In addition to a comprehensive instrument characterization we present an example monsoon plume crossing flight as a case study to demonstrate the instrument capability. Hydrocarbon, halocarbon and oxygenated VOC data from SOFIA are compared with mixing ratios of carbon monoxide (CO) and methane (CH4), used to define the pollution plume. By using excess (ExMR) and normalized excess mixing ratios (NEMRs) the pollution could be attributed to two air masses of distinctly different origin, identified by back-trajectory analysis. This work

  20. Evaluation of pesticide adsorption in gas chromatographic injector and column

    Directory of Open Access Journals (Sweden)

    Gevany Paulino de Pinho

    2012-01-01

    Full Text Available Components in complex matrices can cause variations in chromatographic response during analysis of pesticides by gas chromatography. These variations are related to the competition between analytes and matrix components for adsorption sites in the chromatographic system. The capacity of the pesticides chlorpyrifos and deltamethrin to be adsorbed in the injector and chromatographic column was evaluated by constructing three isotherms and changing the column heating rate to 10 and 30 ºC min-1. By using ANCOVA to compare the slope of calibration graphs, results showed that the higher the injector temperature (310 ºC the lower the pesticide adsorption. Also, deltamethrin influenced the adsorption of chlorpyrifos on the column chromatographic.

  1. Determination of the analytical performance of a headspace capillary gas chromatographic technique and karl Fischer coulometric titration by system calibration using oil samples containing known amounts of moisture.

    Science.gov (United States)

    Jalbert, J; Gilbert, R; Tétreault, P

    1999-08-01

    Over the past few years, concerns have been raised in the literature about the accuracy of the Karl Fischer (KF) method for assessing moisture in transformer mineral oils. To better understand this issue, the performance of a static headspace capillary gas chromatographic (HS-CGC) technique was compared to that of KF coulometric titration by analyzing moisture in samples containing known amounts of water and various samples obtained from the National Institute of Standards and Technology (NIST). Two modes of adding samples into the KF vessel were used:  direct injection and indirect injection via an azeotropic distillation of the moisture with toluene. Under the conditions used for direct injection, the oil matrix was totally dissolved in the anolyte, which allowed the moisture to be titrated in a single-phase solution rather than in a suspension. The results have shown that when HS-CGC and combined azeotropic distillation/KF titration are calibrated with moisture-in-oil standards, a linear relation is observed over 0-60 ppm H(2)O with a correlation coefficient better than 0.9994 (95% confidence), with the regression line crossing through zero. A similar relation can also be observed when calibration is achieved by direct KF addition of standards prepared with octanol-1, but in this case an intercept of 4-5 ppm is noted. The amount of moisture determined by curve interpolation in NIST reference materials by the three calibrated systems ranges from 13.0 to 14.8 ppm for RM 8506 and 42.5 to 46.4 ppm for RM 8507, and in any case, the results were as high as those reported in the literature with volumetric KF titration. However, titration of various dehydrated oil and solvent samples showed that direct KF titration is affected by a small bias when samples contain very little moisture. The source of error after correction for the large sample volume used for the determination (8 mL) is about 6 ppm for Voltesso naphthenic oil and 4 ppm for toluene, revealing a matrix

  2. In Portugal, the energy revolution is underway

    International Nuclear Information System (INIS)

    Mary, Olivier

    2015-01-01

    As at the beginning of 2013, 70 per cent of the electricity consumed in Portugal had a renewable origin, this article outlines that this result is based on a strong-willed policy. In fact, Portugal entered the energy transition in 2001 with its '4E program' (energy efficiency and endogenous energies) which aimed at reaching 60 per cent of renewable energies in electricity consumption by 2020. This program was based on a strong development of wind and hydraulic energy. Moreover, the country developed its own capacities for the manufacturing of wind turbines. On another hand, other renewable energies (notably solar energy) seem a bit late although several projects are underway. As far as hydraulic energy, a dam project is a matter of controversy

  3. Determination of solute descriptors by chromatographic methods

    International Nuclear Information System (INIS)

    Poole, Colin F.; Atapattu, Sanka N.; Poole, Salwa K.; Bell, Andrea K.

    2009-01-01

    The solvation parameter model is now well established as a useful tool for obtaining quantitative structure-property relationships for chemical, biomedical and environmental processes. The model correlates a free-energy related property of a system to six free-energy derived descriptors describing molecular properties. These molecular descriptors are defined as L (gas-liquid partition coefficient on hexadecane at 298 K), V (McGowan's characteristic volume), E (excess molar refraction), S (dipolarity/polarizability), A (hydrogen-bond acidity), and B (hydrogen-bond basicity). McGowan's characteristic volume is trivially calculated from structure and the excess molar refraction can be calculated for liquids from their refractive index and easily estimated for solids. The remaining four descriptors are derived by experiment using (largely) two-phase partitioning, chromatography, and solubility measurements. In this article, the use of gas chromatography, reversed-phase liquid chromatography, micellar electrokinetic chromatography, and two-phase partitioning for determining solute descriptors is described. A large database of experimental retention factors and partition coefficients is constructed after first applying selection tools to remove unreliable experimental values and an optimized collection of varied compounds with descriptor values suitable for calibrating chromatographic systems is presented. These optimized descriptors are demonstrated to be robust and more suitable than other groups of descriptors characterizing the separation properties of chromatographic systems.

  4. Determination of solute descriptors by chromatographic methods.

    Science.gov (United States)

    Poole, Colin F; Atapattu, Sanka N; Poole, Salwa K; Bell, Andrea K

    2009-10-12

    The solvation parameter model is now well established as a useful tool for obtaining quantitative structure-property relationships for chemical, biomedical and environmental processes. The model correlates a free-energy related property of a system to six free-energy derived descriptors describing molecular properties. These molecular descriptors are defined as L (gas-liquid partition coefficient on hexadecane at 298K), V (McGowan's characteristic volume), E (excess molar refraction), S (dipolarity/polarizability), A (hydrogen-bond acidity), and B (hydrogen-bond basicity). McGowan's characteristic volume is trivially calculated from structure and the excess molar refraction can be calculated for liquids from their refractive index and easily estimated for solids. The remaining four descriptors are derived by experiment using (largely) two-phase partitioning, chromatography, and solubility measurements. In this article, the use of gas chromatography, reversed-phase liquid chromatography, micellar electrokinetic chromatography, and two-phase partitioning for determining solute descriptors is described. A large database of experimental retention factors and partition coefficients is constructed after first applying selection tools to remove unreliable experimental values and an optimized collection of varied compounds with descriptor values suitable for calibrating chromatographic systems is presented. These optimized descriptors are demonstrated to be robust and more suitable than other groups of descriptors characterizing the separation properties of chromatographic systems.

  5. Miniaturized chromatographic radiochemical procedure for 131I - MIBG

    International Nuclear Information System (INIS)

    Barboza, M.F. de; Pereira, N.S. de; Colturato, M.T.; Silva, C.P.G. da.

    1989-12-01

    Different solvents were used in paper chromatographic methods to obtain the best system in routine radiochemical control for 131 I-MIBG produced at IPEN-CNEN/SP. The dates were compared with those obtained with eletrophoresis method in buffer acetate, pH=4.5, 350V, during 40 minutes. The stability of the labeled compound store under 4 0 C was studied during 15 days. Miniaturized chromatographic procedures were established using Whatman 3MM (8x1cm) and n-butanol-:acetic acid: water (S:2:1) as a solvent. the Rf values were: 0.3 (I - ) and 1.0 (MIBG). The radiochemical purity was 99.3 and 99.2% (first day) obtained with eletrophoresis and miniaturized chromatographic procedures, respectively and, 84.7% after 15 days of its preparation. It is a rapid, practical and reproductive method. (author) [pt

  6. Research Ship Atlantic Explorer Underway Meteorological Data, Quality Controlled

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Research Ship Atlantic Explorer Underway Meteorological Data (delayed ~10 days for quality control) are from the Shipboard Automated Meteorological and Oceanographic...

  7. Research Ship Nathaniel B. Palmer Underway Meteorological Data, Quality Controlled

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Research Ship Nathaniel B. Palmer Underway Meteorological Data (delayed ~10 days for quality control) are from the Shipboard Automated Meteorological and...

  8. Research Ship Robert Gordon Sproul Underway Meteorological Data, Quality Controlled

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Research Ship Robert Gordon Sproul Underway Meteorological Data (delayed ~10 days for quality control) are from the Shipboard Automated Meteorological and...

  9. Research Ship Roger Revelle Underway Meteorological Data, Quality Controlled

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Research Ship Roger Revelle Underway Meteorological Data (delayed ~10 days for quality control) are from the Shipboard Automated Meteorological and Oceanographic...

  10. NOAA Ship Bell M. Shimada Underway Meteorological Data, Quality Controlled

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NOAA Ship Bell M. Shimada Underway Meteorological Data (delayed ~10 days for quality control) are from the Shipboard Automated Meteorological and Oceanographic...

  11. Research Ship New Horizon Underway Meteorological Data, Quality Controlled

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Research Ship New Horizon Underway Meteorological Data (delayed ~10 days for quality control) are from the Shipboard Automated Meteorological and Oceanographic...

  12. Research Ship Aurora Australis Underway Meteorological Data, Quality Controlled

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Research Ship Aurora Australis Underway Meteorological Data (delayed ~10 days for quality control) are from the Shipboard Automated Meteorological and Oceanographic...

  13. NOAA Ship David Starr Jordan Underway Meteorological Data, Quality Controlled

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NOAA Ship David Starr Jordan Underway Meteorological Data (delayed ~10 days for quality control) are from the Shipboard Automated Meteorological and Oceanographic...

  14. NOAA Ship Henry B. Bigelow Underway Meteorological Data, Quality Controlled

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NOAA Ship Henry B. Bigelow Underway Meteorological Data (delayed ~10 days for quality control) are from the Shipboard Automated Meteorological and Oceanographic...

  15. NOAA Ship Oscar Elton Sette Underway Meteorological Data, Quality Controlled

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NOAA Ship Oscar Elton Sette Underway Meteorological Data (delayed ~10 days for quality control) are from the Shipboard Automated Meteorological and Oceanographic...

  16. Research Ship Southern Surveyor Underway Meteorological Data, Quality Controlled

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Research Ship Southern Surveyor Underway Meteorological Data (delayed ~10 days for quality control) are from the Shipboard Automated Meteorological and Oceanographic...

  17. Research Ship T. G. Thompson Underway Meteorological Data, Quality Controlled

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Research Ship T. G. Thompson Underway Meteorological Data (delayed ~10 days for quality control) are from the Shipboard Automated Meteorological and Oceanographic...

  18. Research Ship Laurence M. Gould Underway Meteorological Data, Quality Controlled

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Research Ship Laurence M. Gould Underway Meteorological Data (delayed ~10 days for quality control) are from the Shipboard Automated Meteorological and Oceanographic...

  19. NOAA Ship Okeanos Explorer Underway Meteorological Data, Quality Controlled

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NOAA Ship Okeanos Explorer Underway Meteorological Data (delayed ~10 days for quality control) are from the Shipboard Automated Meteorological and Oceanographic...

  20. Environmental Technology Verification Report. Field Portable Gas Chromatograph/Mass Spectrometer. Viking Instruments Corporation SpectraTrak (Trademark) 672

    National Research Council Canada - National Science Library

    Enfield, Wayne

    1997-01-01

    .... This self-contained, field transportable system, whose design has been adapted from laboratory technology, uses a contained, chromatographic column and accompanying mass spectrometer to provide...

  1. Isotope separations using chromatographic methods

    International Nuclear Information System (INIS)

    Leseticky, L.

    1985-01-01

    A survey is given of chromatographic separations of compounds only differing in isotope composition. Isotope effects on physical properties which allow chromatographic separation (vapour tension, adsorption heat, partition coefficient) are very small, with the exception of the simplest molecules. Therefore, separation factors only assume the value of several per cent. From this ensues the necessity of using columns which are specially and very carefully prepared and have a separation efficiency of the order of 10 4 theoretical plates. Briefly discussed is liquid chromatography on ion exchangers which with a varied degree of success was used for separating simple inorganic compounds or ions. Ion exchange chromatography of amino acids labelled with tritium, and chromatography of tritium labelled steroids also provided only a certain degree of separation. A detailed analysis is presented of gas chromatography separation of various deuterium and tritium labelled low-molecular compounds, to which a number of studies has been devoted in the literature. Very promising is the method of complexation gas chromatography based on the reversible formation of a complex of the ligand (the compound being separated) and the compound of the (transition) metal as the steady-state phase. (author)

  2. Chemometric technique for the optimization of chromatographic system: Simultaneous HPLC determination of Rosuvastatin, Telmisartan, Ezetimibe and Atorvastatin used in combined cardiovascular therapy

    Directory of Open Access Journals (Sweden)

    V. Sree Janardhanan

    2016-11-01

    Full Text Available Developed and optimized a validated isocratic reverse phase HPLC separation of Rosuvastatin, Telmisartan, Ezetimibe and Atorvastatin in pharmaceutical preparation using response surface methodology. The separation was carried out by using phenomenex C18 column (15 cm × 4.6 mm id, 5 μm particle size and UV detection at 239 nm. The ranges of the independent variables used for the optimization were MeCN: 33–38%, buffer conc.: 10–20 mM and flow rate: 1–2 ml/min. The influence of these independent variables on the output responses: capacity factor of the first peak (k1, resolutions of the 2nd and 3rd peak (Rs2,3, and capacity factor of the fifth peak (k5 were evaluated. Using this strategy, a mathematical model was defined and a response surface was derived for the separation. The three responses were simultaneously optimized by using Derringer's desirability functions. Optimum conditions chosen for the assay were MeCN, MeOH, 20 mM K2HPO4 (pH 3.0 ± 0.2 solution (34.27:20:45.73 v/v/v and flow rate 2 ml/min. Total chromatographic analysis time per sample was approximately 10 min. The optimized assay condition was validated as per the ICH guidelines and applied for the quantitative analysis of Rosavel EZ, Avas-EZ and Lipisar 20 tablet. The developed method was simple, accurate and precise. Hence, it can be employed for the routine analysis in quality control laboratories.

  3. Partition efficiencies of newly fabricated universal high-speed counter-current chromatograph for separation of two different types of sugar derivatives with organic-aqueous two-phase solvent systems

    Science.gov (United States)

    Shinomiya, Kazufusa; Sato, Kazuki; Yoshida, Kazunori; Tokura, Koji; Maruyama, Hiroshi; Yanagidaira, Kazuhiro; Ito, Yoichiro

    2013-01-01

    Universal high-speed counter-current chromatograph (HSCCC) was newly designed and fabricated in our laboratory. It holds a set of four column holders symmetrically around the rotary frame at a distance of 11.2 cm from the central axis. By engaging the stationary gear on the central axis of the centrifuge to the planetary gears on the column holder shaft through a set of idle gears, two pairs of diagonally located column holders simultaneously rotate about their own axes in the opposite directions: one forward (type-J planetary motion) and the other backward (type-I planetary motion) each synchronously with the revolution. Using the eccentric coil assembly, partition efficiencies produced by these two planetary motions were compared on the separation of two different types of sugar derivatives (4-methylumbelliferyl and 5-bromo-4-chloro-3-indoxyl sugar derivatives) using organic-aqueous two-phase solvent systems composed of n-hexane/ethyl acetate/1-butanol/methanol/water and aqueous 0.1 M sodium tetraborate, respectively. With lower phase mobile, better peak resolution was obtained by the type-J forward rotation for both samples probably due to higher retention of the stationary phase. With upper phase mobile, however, similar peak resolutions were obtained between these two planetary motions for both sugar derivatives. The overall results indicate that the present universal HSCCC is useful for counter-current chromatographic separation since each planetary motion has its specific applications: e.g., vortex CCC by the type-I planetary motion and HSCCC by the type-J planetary motion both for separation of various natural and synthetic products. PMID:24267319

  4. Chemometric strategy for automatic chromatographic peak detection and background drift correction in chromatographic data.

    Science.gov (United States)

    Yu, Yong-Jie; Xia, Qiao-Ling; Wang, Sheng; Wang, Bing; Xie, Fu-Wei; Zhang, Xiao-Bing; Ma, Yun-Ming; Wu, Hai-Long

    2014-09-12

    Peak detection and background drift correction (BDC) are the key stages in using chemometric methods to analyze chromatographic fingerprints of complex samples. This study developed a novel chemometric strategy for simultaneous automatic chromatographic peak detection and BDC. A robust statistical method was used for intelligent estimation of instrumental noise level coupled with first-order derivative of chromatographic signal to automatically extract chromatographic peaks in the data. A local curve-fitting strategy was then employed for BDC. Simulated and real liquid chromatographic data were designed with various kinds of background drift and degree of overlapped chromatographic peaks to verify the performance of the proposed strategy. The underlying chromatographic peaks can be automatically detected and reasonably integrated by this strategy. Meanwhile, chromatograms with BDC can be precisely obtained. The proposed method was used to analyze a complex gas chromatography dataset that monitored quality changes in plant extracts during storage procedure. Copyright © 2014 Elsevier B.V. All rights reserved.

  5. PARTITION EFFICIENCY OF NEWLY DESIGNED LOCULAR MULTILAYER COIL FOR COUNTERCURRENT CHROMATOGRAPHIC SEPARATION OF PROTEINS USING SMALL-SCALE CROSS-AXIS COIL PLANET CENTRIFUGE WITH AQUEOUS-AQUEOUS POLYMER PHASE SYSTEMS.

    Science.gov (United States)

    Shinomiya, Kazufusa; Ito, Yoichiro

    2009-01-01

    Countercurrent chromatographic performance of the locular multilayer coil separation column newly designed in our laboratory was evaluated in terms of theoretical plate number, peak resolution and retention of the stationary phase in protein separation with an aqueous polymer phase system using the small-scale cross-axis coil planet centrifuge (X-axis CPC) fabricated in our laboratory. The locular column was made from 1.0 mm I.D., 2.0 mm O.D. or 1.5 mm I.D., 2.5 mm O.D. PTFE tubing compressed with a pair of hemostat at 2 or 4 cm intervals. The protein separation was performed using a set of stable proteins including cytochrome C, myoglobin and lysozyme with the 12.5% (w/w) polyethylene glycol 1000 and 12.5% (w/w) dibasic potassium phosphate system under 1000 rpm of column revolution. The 1.5 mm I.D., 2.5 mm O.D. locular tubing compressed at 2 cm intervals yielded better partition efficiencies than the non-clamped tubing using both lower and upper mobile phases with satisfactory retention of the stationary phase. The overall results suggest that the newly designed locular multilayer coil is useful to the preparative separation of proteins with aqueous-aqueous polymer phase system using our small-scale X-axis CPC.

  6. Chromatographic and biological aspects of organomercurials

    Energy Technology Data Exchange (ETDEWEB)

    Fishbein, L

    1970-01-01

    A thorough review on the biological and chromatographic aspects of methylmercury, phenylmercurials, and miscellaneous organomercurials is presented. Areas covered include ecology, epidemiology, paper chromatography, thin-layer chromatography, gas chromatography, metabolism and toxicity, and environmental degradation. 183 references.

  7. A Quantitative Gas Chromatographic Ethanol Determination.

    Science.gov (United States)

    Leary, James J.

    1983-01-01

    Describes a gas chromatographic experiment for the quantitative determination of volume percent ethanol in water ethanol solutions. Background information, procedures, and typical results are included. Accuracy and precision of results are both on the order of two percent. (JN)

  8. Underway meteorological, navigational, physical and time series data collected aboard NOAA Ship Oscar Elton Sette in the North Pacific Ocean on 2016-06-22 (NCEI Accession 0155170)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0155170 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...

  9. Underway meteorological, navigational, physical and time series data collected aboard NOAA Ship Ronald H. Brown in the Bering Sea on 2015-09-04 (NCEI Accession 0137446)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0137446 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...

  10. Underway meteorological, navigational, physical and time series data collected aboard NOAA Ship Henry B. Bigelow in the North Atlantic Ocean on 2016-08-26 (NCEI Accession 0162238)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0162238 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...

  11. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Gordon Gunter in the Gulf of Mexico on 2016-10-01 (NCEI Accession 0164087)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0164087 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  12. Chromatographic Separations of Enantiomers and Underivatized Oligosaccharides

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Ying [Iowa State Univ., Ames, IA (United States)

    2004-01-01

    My graduate research has focused on separation science and bioanalytical analysis, which emphasized in method development. It includes three major areas: enantiomeric separations using high performance liquid chromatography (HPLC), Super/subcritical fluid chromatography (SFC), and capillary electrophoresis (CE); drug-protein binding behavior studies using CE; and carbohydrate analysis using liquid chromatograph-electrospray ionization mass spectrometry (LC-ESI-MS). Enantiomeric separations continue to be extremely important in the pharmaceutical industry. An in-depth evaluation of the enantiomeric separation capabilities of macrocyclic glycopeptides CSPs with SFC mobile phases was investigated using a set of over 100 chiral compounds. It was found that the macrocyclic based CSPs were able to separate enantiomers of various compounds with different polarities and functionalities. Seventy percent of all separations were achieved in less than 4 min due to the high flow rate (4.0 ml/min) that can be used in SFC. Drug-protein binding is an important process in determining the activity and fate of a drug once it enters the body. Two drug/protein systems have been studied using frontal analysis CE method. More sensitive fluorescence detection was introduced in this assay, which overcame the problem of low sensitivity that is common when using UV detection for drug-protein studies. In addition, the first usage of an argon ion laser with 257 nm beam coupled with CCD camera as a frontal analysis detection method enabled the simultaneous observation of drug fluorescence as well as the protein fluorescence. LC-ESI-MS was used for the separation and characterization of underivatized oligosaccharide mixtures. With the limits of detection as low as 50 picograms, all individual components of oligosaccharide mixtures (up to 11 glucose-units long) were baseline resolved on a Cyclobond I 2000 column and detected using ESI-MS. This system is characterized by high chromatographic

  13. Chromatographic Separations of Enantiomers and Underivatized Oligosaccharides

    International Nuclear Information System (INIS)

    Ying Liu

    2004-01-01

    My graduate research has focused on separation science and bioanalytical analysis, which emphasized in method development. It includes three major areas: enantiomeric separations using high performance liquid chromatography (HPLC), Super/subcritical fluid chromatography (SFC), and capillary electrophoresis (CE); drug-protein binding behavior studies using CE; and carbohydrate analysis using liquid chromatograph-electrospray ionization mass spectrometry (LC-ESI-MS). Enantiomeric separations continue to be extremely important in the pharmaceutical industry. An in-depth evaluation of the enantiomeric separation capabilities of macrocyclic glycopeptides CSPs with SFC mobile phases was investigated using a set of over 100 chiral compounds. It was found that the macrocyclic based CSPs were able to separate enantiomers of various compounds with different polarities and functionalities. Seventy percent of all separations were achieved in less than 4 min due to the high flow rate (4.0 ml/min) that can be used in SFC. Drug-protein binding is an important process in determining the activity and fate of a drug once it enters the body. Two drug/protein systems have been studied using frontal analysis CE method. More sensitive fluorescence detection was introduced in this assay, which overcame the problem of low sensitivity that is common when using UV detection for drug-protein studies. In addition, the first usage of an argon ion laser with 257 nm beam coupled with CCD camera as a frontal analysis detection method enabled the simultaneous observation of drug fluorescence as well as the protein fluorescence. LC-ESI-MS was used for the separation and characterization of underivatized oligosaccharide mixtures. With the limits of detection as low as 50 picograms, all individual components of oligosaccharide mixtures (up to 11 glucose-units long) were baseline resolved on a Cyclobond I 2000 column and detected using ESI-MS. This system is characterized by high chromatographic

  14. Studies on polar high-speed counter-current chromatographic systems in separation of amaranthine-type betacyanins from Celosia species.

    Science.gov (United States)

    Spórna-Kucab, Aneta; Milo, Angelika; Kumorkiewicz, Agnieszka; Wybraniec, Sławomir

    2018-01-15

    Betacyanins, natural plant pigments exhibiting antioxidant and chemopreventive properties, were extracted from Celosia spicata (Thouars) Spreng. inflorescences and separated by high-speed counter-current chromatography (HSCCC) in two polar solvent systems composed of: TBME - 1-BuOH - ACN - H 2 O (0.7% HFBA, 2:2:1:5, v/v/v/v) (system I) and EtOH - ACN - 1-PrOH - (NH 4 ) 2 SO 4satd.soln - H 2 O (0.5:0.5:0.5:1.2:1, v/v/v/v/v) (system II). The systems were used in the head-to-tail (system I) and tail-to-head (system II) mode. The flow rate of the mobile phase was 2.0 ml/min and the column rotation speed was 860 rpm. The retention of the stationary phase was 73.5% (system I) and 80.0% (system II). For the identification of separated betacyanins in the crude extract as well as in the HSCCC fractions, LC-DAD-ESI-MS/MS analyses were performed. Depending on the target compounds, each of the systems exhibit meaningfully different selectivity and applicability. For the pairs of amaranthines (1/1') and betanins (2/2'), the best choice is the system II, but the acylated amaranthine pairs (3/3' and 4/4') can be resolved only in the ion-pair system I. For the indication of the most suitable solvent system for Celosia plumosa hort., Celosia cristata L. and Celosia spicata (Thouars) Spreng. species, the profiles of betacyanins in different plant parts were studied. Copyright © 2017. Published by Elsevier B.V.

  15. Partition Efficiency of High-Pitch Locular Multilayer Coil for Countercurrent Chromatographic Separation of Proteins Using Small-Scale Cross-Axis Coil Planet Centrifuge and Application to Purification of Various Collagenases with Aqueous-Aqueous Polymer Phase Systems

    Science.gov (United States)

    Shinomiya, Kazufusa; Kobayashi, Hiroko; Inokuchi, Norio; Nakagomi, Kazuya; Ito, Yoichiro

    2010-01-01

    Partition efficiency of the high-pitch locular multilayer coil was evaluated in countercurrent chromatographic (CCC) separation of proteins with an aqueous-aqueous polymer phase system using the small-scale cross-axis coil planet centrifuge (X-axis CPC) fabricated in our laboratory. The separation column was specially made by high-pitch (ca 5 cm) winding of 1.0 mm I.D., 2.0 mm O.D. locular tubing compressed at 2 cm intervals with a total capacity of 29.5 mL. The protein separation was performed using a set of stable proteins including cytochrome C, myoglobin, and lysozyme with the 12.5% (w/w) polyethylene glycol (PEG) 1000 and 12.5% (w/w) dibasic potassium phosphate system (pH 9.2) under 1000 rpm of column revolution. This high-pitch locular tubing yielded substantially increased stationary phase retention than the normal locular tubing for both lower and upper mobile phases. In order to demonstrate the capability of the high-pitch locular tubing, the purification of collagenase from the crude commercial sample was carried out using an aqueous-aqueous polymer phase system. Using the 16.0% (w/w) PEG 1000 – 6.3% (w/w) dibasic potassium phosphate – 6.3% (w/w) monobasic potassium phosphate system (pH 6.6), collagenase I, II, V and X derived from Clostridium hystolyticum were separated from other proteins and colored small molecular weight compounds present in the crude commercial sample, while collagenase N-2 and S-1 from Streptomyces parvulus subsp. citrinus were eluted with impurities at the solvent front with the upper phase. The collagenase from C. hystolyticum retained its enzymatic activity in the purified fractions. The overall results demonstrated that the high-pitch locular multilayer coil is effectively used for the CCC purification of bioactive compounds without loss of their enzymatic activities. PMID:21869859

  16. Partition Efficiency of High-Pitch Locular Multilayer Coil for Countercurrent Chromatographic Separation of Proteins Using Small-Scale Cross-Axis Coil Planet Centrifuge and Application to Purification of Various Collagenases with Aqueous-Aqueous Polymer Phase Systems.

    Science.gov (United States)

    Shinomiya, Kazufusa; Kobayashi, Hiroko; Inokuchi, Norio; Nakagomi, Kazuya; Ito, Yoichiro

    2011-01-01

    Partition efficiency of the high-pitch locular multilayer coil was evaluated in countercurrent chromatographic (CCC) separation of proteins with an aqueous-aqueous polymer phase system using the small-scale cross-axis coil planet centrifuge (X-axis CPC) fabricated in our laboratory. The separation column was specially made by high-pitch (ca 5 cm) winding of 1.0 mm I.D., 2.0 mm O.D. locular tubing compressed at 2 cm intervals with a total capacity of 29.5 mL. The protein separation was performed using a set of stable proteins including cytochrome C, myoglobin, and lysozyme with the 12.5% (w/w) polyethylene glycol (PEG) 1000 and 12.5% (w/w) dibasic potassium phosphate system (pH 9.2) under 1000 rpm of column revolution. This high-pitch locular tubing yielded substantially increased stationary phase retention than the normal locular tubing for both lower and upper mobile phases. In order to demonstrate the capability of the high-pitch locular tubing, the purification of collagenase from the crude commercial sample was carried out using an aqueous-aqueous polymer phase system. Using the 16.0% (w/w) PEG 1000 - 6.3% (w/w) dibasic potassium phosphate - 6.3% (w/w) monobasic potassium phosphate system (pH 6.6), collagenase I, II, V and X derived from Clostridium hystolyticum were separated from other proteins and colored small molecular weight compounds present in the crude commercial sample, while collagenase N-2 and S-1 from Streptomyces parvulus subsp. citrinus were eluted with impurities at the solvent front with the upper phase. The collagenase from C. hystolyticum retained its enzymatic activity in the purified fractions. The overall results demonstrated that the high-pitch locular multilayer coil is effectively used for the CCC purification of bioactive compounds without loss of their enzymatic activities.

  17. Correlations between chromatographic parameters and bioactivity predictors of potential herbicides.

    Science.gov (United States)

    Janicka, Małgorzata

    2014-08-01

    Different liquid chromatography techniques, including reversed-phase liquid chromatography on Purosphere RP-18e, IAM.PC.DD2 and Cosmosil Cholester columns and micellar liqud chromatography with a Purosphere RP-8e column and using buffered sodium dodecyl sulfate-acetonitrile as the mobile phase, were applied to study the lipophilic properties of 15 newly synthesized phenoxyacetic and carbamic acid derivatives, which are potential herbicides. Chromatographic lipophilicity descriptors were used to extrapolate log k parameters (log kw and log km) and log k values. Partitioning lipophilicity descriptors, i.e., log P coefficients in an n-octanol-water system, were computed from the molecular structures of the tested compounds. Bioactivity descriptors, including partition coefficients in a water-plant cuticle system and water-human serum albumin and coefficients for human skin partition and permeation were calculated in silico by ACD/ADME software using the linear solvation energy relationship of Abraham. Principal component analysis was applied to describe similarities between various chromatographic and partitioning lipophilicities. Highly significant, predictive linear relationships were found between chromatographic parameters and bioactivity descriptors. © The Author [2013]. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  18. Evaluation of chromatographic conditions in reversed phase liquid chromatography-mass spectrometry systems for fingerprinting of polar and amphiphilic plant metabolites

    DEFF Research Database (Denmark)

    Nielsen, Nikoline Juul; Tomasi, Giorgio; Christensen, Jan H.

    2016-01-01

    Metabolic fingerprinting is a relatively young scientific discipline requiring robust, yet flexible and fit-for-purpose analytical methods. Here, we introduce a simple approach to select reversed phase LC systems with electrospray MS detection for fingerprinting of polar and amphiphilic plant met...

  19. A Generalized Adsorption Rate Model Based on the Limiting-Component Constraint in Ion-Exchange Chromatographic Separation for Multicomponent Systems

    DEFF Research Database (Denmark)

    such that conventional LDF (linear driving force) type models are extended to inactive zones without loosing their generality. Based on a limiting component constraint, an exchange probability kernel is developed for multi-component systems. The LDF-type model with the kernel is continuous with time and axial direction....... Two tuning parameters such as concentration layer thickness and function change rate at the threshold point are needed for the probability kernels, which are not sensitive to problems considered....

  20. Optimization of the gas chromatographic separations

    International Nuclear Information System (INIS)

    Gasco Sanchez, L.

    1973-01-01

    A review and a critical study on the optimization of the gas chromatographic separations are made. After dealing with the fundamental gas chromatographic equations, some methods of expressing column performances are discussed: performance indices, performance parameters, resolution and effective plate number per unit time. This is completed with a comparative study on performances of various types of columns. Moreover, optimization methods for operating chromatographic conditions are extensively dealt with: as resolution optimization, separation time, and normalization techniques for the time of analysis in order to achieve the maximum resolution at constant time. Finally, some others non operating parameters such as: selectivity of stationary phases, column preparation and optimization methods by means of computers are studied. (Author) 68 refs

  1. Chromatographic studies on thermal and radiolytic degradation of n-dodecane-HNO3 two components system and its purification employing silica gel treatment

    International Nuclear Information System (INIS)

    Kadam, Prashant; Kaushik, C.P.; Ozarde, P.D.; Bindu, M.; Tripathi, S.C.; Jambunathan, U.; Pandit, G.G.

    2005-01-01

    This paper describes the studies carried out to examine the extent of degradation of n-dodecane at elevated temperature for different time periods and the effect of gamma radiolysis at different absorbed dose, in n-dodecane- nitric acid two components system. The studies also involved the identification of the degradation products formed during above process using GC-MS. A large number of degradation products were observed. Formation of degradation products increases with absorbed dose and time of heating. Further purification of degraded dodecane was carried out with silica gel crystals as an adsorbent. Treated dodecane samples were subjected to GC/GC-MS analysis, to confirm the extent of removal degradation products. (author)

  2. Determination of descriptors for polycyclic aromatic hydrocarbons and related compounds by chromatographic methods and liquid-liquid partition in totally organic biphasic systems.

    Science.gov (United States)

    Ariyasena, Thiloka C; Poole, Colin F

    2014-09-26

    Retention factors on several columns and at various temperatures using gas chromatography and from reversed-phase liquid chromatography on a SunFire C18 column with various mobile phase compositions containing acetonitrile, methanol and tetrahydrofuran as strength adjusting solvents are combined with liquid-liquid partition coefficients in totally organic biphasic systems to calculate descriptors for 23 polycyclic aromatic hydrocarbons and eighteen related compounds of environmental interest. The use of a consistent protocol for the above measurements provides descriptors that are more self consistent for the estimation of physicochemical properties (octanol-water, air-octanol, air-water, aqueous solubility, and subcooled liquid vapor pressure). The descriptor in this report tend to have smaller values for the L and E descriptors and random differences in the B and S descriptors compared with literature sources. A simple atom fragment constant model is proposed for the estimation of descriptors from structure for polycyclic aromatic hydrocarbons. The new descriptors show no bias in the prediction of the air-water partition coefficient for polycyclic aromatic hydrocarbons unlike the literature values. Copyright © 2014 Elsevier B.V. All rights reserved.

  3. Liquid chromatographic-mass spectrometric method for ...

    African Journals Online (AJOL)

    Tropical Journal of Pharmaceutical Research ... Purpose: To develop and validate a simple, efficient and reliable Liquid chromatographic-mass spectrometric (LC-MS/MS) method for the quantitative determination of two dermatological drugs, ... By Country · List All Titles · Free To Read Titles This Journal is Open Access.

  4. Liquid chromatographic analysis of phenobarbitone, ethosuximide ...

    African Journals Online (AJOL)

    A liquid chromatographic method for the simultaneous assay of four anticonvulsant drugs, phenobarbitone, ethosuximide, phenytoin and carbamazepine on a polystyrene-divinyl benzene column is described. The method was developed by the systematic study of different types of co-polymer materials, type and ...

  5. Validated high performance liquid chromatographic (HPLC) method ...

    African Journals Online (AJOL)

    STORAGESEVER

    2010-02-22

    Feb 22, 2010 ... specific and accurate high performance liquid chromatographic method for determination of ZER in micro-volumes ... tional medicine as a cure for swelling, sores, loss of appetite and ... Receptor Activator for Nuclear Factor κ B Ligand .... The effect of ... be suitable for preclinical pharmacokinetic studies. The.

  6. Chromatographic characterisation, in vitro antioxidant and free ...

    African Journals Online (AJOL)

    Chromatographic fractionation and spectroscopic analysis of the ME4 fraction revealed the presence of four compounds namely garcinia biflavonoids GB1 and GB2, garcinal and garcinoic acid. These findings show that these four compounds are partly responsible for the great antioxidant potential of G. kola seeds.

  7. A Small-Scale Low-Cost Gas Chromatograph

    Science.gov (United States)

    Gros, Natasa; Vrtacnik, Margareta

    2005-01-01

    The design and application of a small-scale portable gas chromatograph for learning of the basic concepts of chromatography is described. The apparatus consists of two basic separable units, which includes a chromatographic unit and an electronic unit.

  8. Effects of chromatographic fractions of Euphorbia hirta on the rat ...

    African Journals Online (AJOL)

    The effects of the chromatographic fractions of Euphorbia hirta Linn on the serum biochemical parameters in rats were investigated. The ethanolic extract of this plant was subjected to chromatographic separation using the vacuum liquid chromatographic technique, a modified form of classical column chromatography.

  9. Chromatographic separation of europium and gadolinum mixtures by ethylenediaminedisuccinic acid

    International Nuclear Information System (INIS)

    Kolleganov, M.Yu.; Nazarov, P.P.; Martynenko, L.I.; Mtrofanova, N.D.; Spitsyn, V.I.

    1985-01-01

    Comparative investigation of chromatographic separation of impurities of Eu and Gd micro- and macroquantities by means of ethylenediaminedisuccinic (EDDS) and ethylene-diaminetetraacetic (EDTA) acids is performed. It is shown that EDTA is a sufficiently effective agent for separation of this pair of elements. The values of EU and Gd separation coefficients obtained in experiments with RE microquantities are not realized at Eu-Gd, macroquantities separation which probably is connected with the influence of polymerization processes in the systems containing RE and EDDS complexes

  10. Gas chromatographic isolation technique for compound-specific radiocarbon analysis

    International Nuclear Information System (INIS)

    Uchida, M.; Kumamoto, Y.; Shibata, Y.; Yoneda, M.; Morita, M.; Kawamura, K.

    2002-01-01

    Full text: We present here a gas chromatographic isolation technique for the compound-specific radiocarbon analysis of biomarkers from the marine sediments. The biomarkers of fatty acids, hydrocarbon and sterols were isolated with enough amount for radiocarbon analysis using a preparative capillary gas chromatograph (PCGC) system. The PCGC systems used here is composed of an HP 6890 GC with FID, a cooled injection system (CIS, Gerstel, Germany), a zero-dead-volume effluent splitter, and a cryogenic preparative collection device (PFC, Gerstel). For AMS analysis, we need to separate and recover sufficient quantity of target individual compounds (>50 μgC). Yields of target compounds from C 14 n-alkanes to C 40 to C 30 n-alkanes and approximately that of 80% for higher molecular weights compounds more than C 30 n-alkanes. Compound specific radiocarbon analysis of organic compounds, as well as compound-specific stable isotope analysis, provide valuable information on the origins and carbon cycling in marine system. Above PCGC conditions, we applied compound-specific radiocarbon analysis to the marine sediments from western north Pacific, which showed the possibility of a useful chronology tool for estimating the age of sediment using organic matter in paleoceanographic study, in the area where enough amounts of planktonic foraminifera for radiocarbon analysis by accelerator mass spectrometry (AMS) are difficult to obtain due to dissolution of calcium carbonate. (author)

  11. Design of a coil satellite centrifuge and its performance on counter-current chromatographic separation of 4-methylumbelliferyl sugar derivatives with polar organic-aqueous two-phase solvent systems.

    Science.gov (United States)

    Shinomiya, Kazufusa; Tokura, Koji; Kimura, Emiru; Takai, Midori; Harikai, Naoki; Yoshida, Kazunori; Yanagidaira, Kazuhiro; Ito, Yoichiro

    2015-05-01

    A new high-speed counter-current chromatograph, named coil satellite centrifuge (CSC), was designed and fabricated in our laboratory. The CSC apparatus produces the satellite motion such that the coiled column simultaneously rotates around the sun axis (the angular velocity, ω1), the planet axis (ω2) and the satellite axis (the central axis of the column) (ω3). In order to achieve this triplicate rotary motion without twisting of the flow tube, the rotation of each axis was determined by the following formula: ω1=ω2+ω3. This relation enabled to lay out the flow tube without twisting by the simultaneous rotation of three axes. The flow tube was introduced from the bottom side of the apparatus into the sun axis of the first rotary frame reaching the upper side of the planet axis and connected to the column in the satellite axis. The performance of the apparatus was examined on separation of 4-methylumbelliferyl (MU) sugar derivatives as test samples with organic-aqueous two-phase solvent systems composed of ethyl acetate/1-butanol/water (3:2:5, v/v) for lower phase mobile and (1:4:5, v/v) for upper phase mobile. With lower phase mobile, five 4-MU sugar derivatives including β-D-cellobioside (Cel), β-D-glucopyranoside, α-D-mannopyranoside, β-D-fucopyranoside and α-L-fucopyranoside (α-L-Fuc) were separated with the combined rotation around each axis at counterclockwise (CCW) (ω1) - CCW (ω2) - CCW (ω3) by the flow tube distribution. With upper phase mobile, three 4-MU sugar derivatives including α-L-Fuc, β-D-galactopyranoside and Cel were separated with the combined rotation around each axis at clockwise (CW) (ω1) - CW (ω2) - CW (ω3) by the flow tube distribution. A series of experiments on peak resolution and stationary phase retention revealed that better partition efficiencies were obtained at the flow rate of 0.5 mL/min (column 1) and 0.8 mL/min (column 2) for lower phase mobile and 0.2 mL/min (column 1) and 0.4 mL/min (column 2) for upper phase

  12. Principles of qualitative analysis in the chromatographic context.

    Science.gov (United States)

    Valcárcel, M; Cárdenas, S; Simonet, B M; Carrillo-Carrión, C

    2007-07-27

    This article presents the state of the art of qualitative analysis in the framework of the chromatographic analysis. After establishing the differences between two main classes of qualitative analysis (analyte identification and sample classification/qualification) the particularities of instrumental qualitative analysis are commented on. Qualitative chromatographic analysis for sample classification/qualification through the so-called chromatographic fingerprint (for complex samples) or the volatiles profile (through the direct coupling headspace-mass spectrometry using the chromatograph as interface) is discussed. Next, more technical exposition of the qualitative chromatographic information is presented supported by a variety of representative examples.

  13. Liquid chromatographic determination of water

    Science.gov (United States)

    Fortier, N.E.; Fritz, J.S.

    1990-11-13

    A sensitive method for the determination of water in the presence of common interferences is presented. The detection system is based on the effect of water on the equilibrium which results from the reaction aryl aldehydes, such as cinnamaldehyde and methanol in the eluent to form cinnamaldehyde dimethylacetal, plus water. This equilibrium is shifted in a catalytic atmosphere of a hydrogen ion form past column reactor. The extent of the shift and the resulting change in absorbance are proportional to the amount of water present. 1 fig.

  14. GAS CHROMATOGRAPHIC AND SPECTROSCOPIC ANALYSIS OF ...

    African Journals Online (AJOL)

    Peroxyformic acid prepared in-situ was employed for epoxidation of canola oil in the presence of toluene. Gas chromatographic analysis of the product revealed the following species: C16:0; C18:0; C18:1; C18:2; C18:3; monoepoxy C18:0; monoepoxy C18:1; monoepoxy C18:2; diepoxy C18:0; diepoxy C18:1 and triepoxy ...

  15. Analyzing chromatographic data using multilevel modeling.

    Science.gov (United States)

    Wiczling, Paweł

    2018-06-01

    It is relatively easy to collect chromatographic measurements for a large number of analytes, especially with gradient chromatographic methods coupled with mass spectrometry detection. Such data often have a hierarchical or clustered structure. For example, analytes with similar hydrophobicity and dissociation constant tend to be more alike in their retention than a randomly chosen set of analytes. Multilevel models recognize the existence of such data structures by assigning a model for each parameter, with its parameters also estimated from data. In this work, a multilevel model is proposed to describe retention time data obtained from a series of wide linear organic modifier gradients of different gradient duration and different mobile phase pH for a large set of acids and bases. The multilevel model consists of (1) the same deterministic equation describing the relationship between retention time and analyte-specific and instrument-specific parameters, (2) covariance relationships relating various physicochemical properties of the analyte to chromatographically specific parameters through quantitative structure-retention relationship based equations, and (3) stochastic components of intra-analyte and interanalyte variability. The model was implemented in Stan, which provides full Bayesian inference for continuous-variable models through Markov chain Monte Carlo methods. Graphical abstract Relationships between log k and MeOH content for acidic, basic, and neutral compounds with different log P. CI credible interval, PSA polar surface area.

  16. Chromatographic 188W →188Re generator

    International Nuclear Information System (INIS)

    Khujaev, S.

    2005-01-01

    Full text: The main purpose of the generator - reception of daughter radioisotope Rhenium-188 from it by periodic elution for a long period of time (more than half-year). It is generally known that Rhenium-188, in the form of its complex connections, is applied in nuclear medicine in treatment and removal of painful syndromes. The generator possesses convenient nuclear-physical characteristics of a daughter radioisotope Rhenium-188. It is a source of (Irradiation with energy 2.12 MeV (98 %) with small contribution soft γ-radiation with energy 0.155 MeV (15 %). The Period of half-life destruction of radioisotope is 17 hours. The 188 W parent radioisotope for the generator is formed by irradiation of 186 W neutrons based on the following reaction: 186 W (n,γ) 187 W (n,γ) 188 W (69 days) → 188 Re (17 hours) + β The following were used as targets for irradiation: 1) Metal Tungsten (powder) of natural structure; 2) Metal Tungsten (plate) of natural structure; 3) Metal Tungsten (wire) of natural structure, d = 12 mm; 4) Metal Tungsten (powder) with enrichment on isotope 186 W - 99.79 %. The irradiated material was exposed to chemical processing with reception of radioactive solution of tungsten-188, from which sorption Tungsten was carried out onto sorbent as poly-wolframate-ions. It is established that Tungsten sorption depends on many factors as there are various chemical forms of Tungsten (VI) in water solutions, ratio of which depends on pH of the solution, concentration of Tungsten in the solution and presence of foreign ions. Tungsten sorption was carried out in static and in dynamic regimes. At dynamic regime the sorbent was placed directly in the generating column. The generator consisted of chromatographic columns with sorbent and radioisotope 188 W, eluting system and radiation protection. Rhenium-188 was taken from the generator as perrhenate sodium by elution of 0.9 % solution of chloride sodium in 10 ml. Technical characteristics of the generator

  17. Gas-chromatographic quantitative determination of argon in air samples, by elimination of oxigen

    International Nuclear Information System (INIS)

    Sofronie, E.

    1982-08-01

    A method of gas-chromatographic quantitative determination of argon in air samples, by elimination of oxygen, is presented. Experiments were carried out in a static system. Conditions for the application of the method in dynamic systems are specified. Sensibility of the method: 5 10 -4 cm 3 Ar per cm 3 of air. (author)

  18. Technical artifacts in chromatographic analysis of Tc-99m radiopharmaceuticals

    International Nuclear Information System (INIS)

    Kowalsky, R.J.; Creekmore, J.R.

    1982-01-01

    Technical artifacts produced during chromatographic analysis of technetium radiopharmaceuticals were investigated. Such artifacts are, we found, caused by improper spotting and drying techniques; these in turn produce spuriously high impurities in Tc-99m complexes of DTPA, MDP, PPi, and GH. The ITLC-SG/acetone system produces considerable streaking of Tc-complex if the applied spot is large and not dried before development. This results in activity in the solvent front portion of the chromatographic strip indicating falsely high levels of pertechnetate impurity. Proper drying of the applied spot eliminates the artifact. The ITLC-SG/saline system yields falsely high, hydrolyzed-reduced technetium impurities if the spot is allowed to enter the solvent during development. Correct spot placement and size eliminate this problem. Strips that are allowed to dry in room air for several minutes may indicate considerable pertechnetate impurity on the chromatogram; yet this may not actually be present in the radiopharmaceutical vial. Drying spots rapidly with hot air or in a nitrogen atmosphere before development eliminates this problem

  19. Chromatographic analysis of wheatgrass extracts

    Directory of Open Access Journals (Sweden)

    Masood Shah Khan

    2015-01-01

    Full Text Available Aim: Wheatgrass (WG is the shoot of Triticum aestivum Linn. belongs to the family Gramineae, and possess high chlorophyll content and essential vitamins, minerals, vital enzymes, amino acids, dietary fibers etc., It has been shown to possess anti-cancer, anti-ulcer, antioxidant, and anti-arthritic activity due to the presence of biologically active compounds, and minerals. Therefore, in the present study, high-performance thin layer chromatography (HPTLC, and high-performance liquid chromatography (HPLC methods for qualitative and quantitative analysis have been proposed, which will help in quality evaluation of wheat grass extract. Materials and Methods: Samples for analysis were prepared in methanol and water simply by sonication. These were applied on pre-coated silica plate and chromatograms were developed using toluene: Ethyl acetate: Formic acid. HPLC analysis was done on Waters HPLC system using water, methanol, and acetonitrile as mobile phase. Merck C18 column has been used. Results: HPTLC finger printing of alcoholic extracts of WG was carried out and found 10–11 spots at different wavelengths 254, 366, and 435 nm. HPLC fingerprinting produced 22 peaks at 256 nm. Quantitative HPTLC analysis was done to determine the gallic acid content, and was found to be 0.077% w/w in aqueous extract. By HPLC, the content of gallic acid and rutin was found to be 0.07%, and 0.04% w/w in aqueous extract of WG. Conclusion: The developed HPLC and HPTLC fingerprinting method can be used for the quality control, and standardization of WG and its extracts used as nutritional supplement.

  20. Use of laminar chromatographic methods for determination of separation conditions in column extraction chromatography

    International Nuclear Information System (INIS)

    Ghersini, G.; Cerrai, E.

    1978-01-01

    Possibilities of using laminar chromatographic methods (paper and thin-layer chromatography) to determine optimal separation conditions in column extraction chromatography are analysed. Most of the given laminar methods are presented as Rf-spectra, i.e. as dependences of Rf found experimentally on eluating solution component concentration. Interrelation between Rf and distribution coefficients of corresponding liquid extraction systems and retention volumes of chromatographic columns is considered. Literature data on extraction paper and thin-layer chromatography of elements with various immovable phases are presented

  1. Ion chromatographic determination of vanadium (4) and vanadium (5) in the form of EDTA complexes

    International Nuclear Information System (INIS)

    Komarova, T.V.; Obrezkov, O.N.; Shpigun, O.A.

    1991-01-01

    Ion chromatographic behaviour of V 4+ and V 5+ -EDTA complexes (1,2) and V 5+ -EDTA monoperoxo complexes (3) has been studied. The regularities of ion exchange are obeyed for 1 and 3 complexes. These complex anions are strongly retained on HIX-1 anion exchanger. The chromatographic characteristics of 2 change in time, therefore its determination is impossible under the conditions studied. A method has been developed of the simultaneous determination of V 4+ and V 5+ which is based on retarded formation of the V 5+ -EDTA monoperoxo complex in a system of V 4+ -EDTA - H 2 O 2

  2. Investigations for chromatographic separation and determination of the rare earths and auxiliary group elements

    International Nuclear Information System (INIS)

    Post, K.

    1981-01-01

    Different method have been developed to quantitatively determine rare earth elements after their column chromatographic separation. The influence of the active solvents in the running systems diethyl ether/tetra hydrofuran (THF)/nitric acid (HNO 3 ) and diethyl ether/bis-(2-ethyl hexyl) phosphate (HDEHP)/HNO 3 was investigated on the column chromatographic separation of all rare earth elements is possible by the synergistic combination of the active running components THF and HDEHP. Further from product isotopes could also be separated using the running agents described here in investigations to separate fission product mixtures of irradiated uranium. (orig./HBR) [de

  3. Chromatographic separation of radioactive noble gases from xenon

    Science.gov (United States)

    Akerib, D. S.; Araújo, H. M.; Bai, X.; Bailey, A. J.; Balajthy, J.; Beltrame, P.; Bernard, E. P.; Bernstein, A.; Biesiadzinski, T. P.; Boulton, E. M.; Bramante, R.; Cahn, S. B.; Carmona-Benitez, M. C.; Chan, C.; Chiller, A. A.; Chiller, C.; Coffey, T.; Currie, A.; Cutter, J. E.; Davison, T. J. R.; Dobi, A.; Dobson, J. E. Y.; Druszkiewicz, E.; Edwards, B. N.; Faham, C. H.; Fiorucci, S.; Gaitskell, R. J.; Gehman, V. M.; Ghag, C.; Gibson, K. R.; Gilchriese, M. G. D.; Hall, C. R.; Hanhardt, M.; Haselschwardt, S. J.; Hertel, S. A.; Hogan, D. P.; Horn, M.; Huang, D. Q.; Ignarra, C. M.; Ihm, M.; Jacobsen, R. G.; Ji, W.; Kamdin, K.; Kazkaz, K.; Khaitan, D.; Knoche, R.; Larsen, N. A.; Lee, C.; Lenardo, B. G.; Lesko, K. T.; Lindote, A.; Lopes, M. I.; Manalaysay, A.; Mannino, R. L.; Marzioni, M. F.; McKinsey, D. N.; Mei, D.-M.; Mock, J.; Moongweluwan, M.; Morad, J. A.; Murphy, A. St. J.; Nehrkorn, C.; Nelson, H. N.; Neves, F.; O'Sullivan, K.; Oliver-Mallory, K. C.; Palladino, K. J.; Pease, E. K.; Pech, K.; Phelps, P.; Reichhart, L.; Rhyne, C.; Shaw, S.; Shutt, T. A.; Silva, C.; Solovov, V. N.; Sorensen, P.; Stephenson, S.; Sumner, T. J.; Szydagis, M.; Taylor, D. J.; Taylor, W.; Tennyson, B. P.; Terman, P. A.; Tiedt, D. R.; To, W. H.; Tripathi, M.; Tvrznikova, L.; Uvarov, S.; Verbus, J. R.; Webb, R. C.; White, J. T.; Whitis, T. J.; Witherell, M. S.; Wolfs, F. L. H.; Yazdani, K.; Young, S. K.; Zhang, C.

    2018-01-01

    The Large Underground Xenon (LUX) experiment operates at the Sanford Underground Research Facility to detect nuclear recoils from the hypothetical Weakly Interacting Massive Particles (WIMPs) on a liquid xenon target. Liquid xenon typically contains trace amounts of the noble radioactive isotopes 85Kr and 39Ar that are not removed by the in situ gas purification system. The decays of these isotopes at concentrations typical of research-grade xenon would be a dominant background for a WIMP search experiment. To remove these impurities from the liquid xenon, a chromatographic separation system based on adsorption on activated charcoal was built. 400 kg of xenon was processed, reducing the average concentration of krypton from 130 ppb to 3.5 ppt as measured by a cold-trap assisted mass spectroscopy system. A 50 kg batch spiked to 0.001 g/g of krypton was processed twice and reduced to an upper limit of 0.2 ppt.

  4. Design of a novel coil satellite centrifuge and its performance on counter-current chromatographic separation of 4-methylumbelliferyl sugar derivatives with organic-aqueous two-phase solvent systems

    Science.gov (United States)

    Shinomiya, Kazufusa; Tokura, Koji; Kimura, Emiru; Takai, Midori; Harikai, Naoki; Yoshida, Kazunori; Yanagidaira, Kazuhiro; Ito, Yoichiro

    2015-01-01

    A new high-speed counter-current chromatograph, named coil satellite centrifuge (CSC), was designed and fabricated in our laboratory. The CSC apparatus produces the satellite motion such that the coiled column simultaneously rotates around the sun axis (the angular velocity, ω1), the planet axis (ω2) and the satellite axis (the central axis of the column) (ω3). In order to achieve this triplicate rotary motion without twisting of the flow tube, the rotation of each axis was determined by the following formula: ω1 = ω2 + ω3. This relation enabled to lay out the flow tube by two different ways, the SS type and the JS type. In the SS type, the flow tube was introduced from the upper side of the apparatus into the sun axis of the first rotary frame and connected to the planet axis of the second rotary frame like a double letter SS. In the JS type, the flow tube was introduced from the bottom of the apparatus into the sun axis reaching the upper side of the planet axis an inversed letter J, followed by distribution as in the SS type. The performance of the apparatus was examined on separation of 4-methylumbelliferyl (MU) sugar derivatives as test samples with organic-aqueous two-phase solvent systems composed of ethyl acetate/1-butanol/water (3 : 2 : 5, v/v) for lower phase mobile and (1 : 4 : 5, v/v) for upper phase mobile. With lower phase mobile, five 4-MU sugar derivatives including β-D-cellobioside (Cel), β-D-glucopyranoside, α-D-mannopyranoside, β-D-fucopyranoside and α-L-fucopyranoside (α-L-Fuc) were separated with the combined rotation around each axis at counterclockwise (CCW) (ω1) – CCW (ω2) – CCW (ω3) by the JS type flow tube distribution. With upper phase mobile, three 4-MU sugar derivatives including α-L-Fuc, β-D-galactopyranoside and Cel were separated with the combined rotation around each axis at clockwise (CW) (ω1) – CW (ω2) – CW (ω3) by the JS type flow tube distribution. A series of experiments on peak resolution and

  5. Preparation and evaluation of 99mTc-cefuroxime, a potential infection specific imaging agent: A reliable thin layer chromatographic system to delineate impurities from the 99mTc-antibiotic

    International Nuclear Information System (INIS)

    Chattopadhyay, Sankha; Ghosh, Mayuri; Sett, Sucharita; Das, Malay Kanti; Chandra, Susmita; De, Kakali; Mishra, Mridula; Sinha, Samarendu; Ranjan Sarkar, Bharat; Ganguly, Shantanu

    2012-01-01

    Technetium-99m labelled cefuroxime, a second-generation cephalosporin antibiotic and potential bacteria specific infection imaging agent was evaluated. A good radiochemical purity (95%) of the labelled product was obtained after filtering the reaction mixture through a 0.22 μm filter. Scintigraphy study of the purified product showed uptake in infectious lesions 45 min after injection and abscess-to-muscle ratios were found to be 1.80, 1.85 and 1.88 at 45 min, 1.5 hr and 3 hr, respectively. A versatile and reliable chromatographic technique to assess the radiochemical purity of 99m Tc-cefuroxime has also been described. - Highlights: ► Preparation of 99m Tc-cefuroxime in presence of Sn-tartrate as reducing agent. ► Physiochemical and biological evaluation of 99m Tc-cefuroxime. ► A versatile and reliable chromatographic technique to assess the radiochemical purity of 99m Tc-cefuroxime.

  6. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Pisces in the Gulf of Mexico from 2013-10-22 to 2013-12-07 (NCEI Accession 0142630)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0142630 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  7. Underway meteorological, navigational, physical and time series data collected aboard NOAA Ship Okeanos Explorer in the North Pacific Ocean from 2015-07-10 to 2015-09-03 (NCEI Accession 0141435)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0141435 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...

  8. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Pisces in the Gulf of Mexico from 2014-05-27 to 2014-09-30 (NODC Accession 0119414)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0119414 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  9. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Reuben Lasker in the North Pacific Ocean from 2016-03-22 to 2016-04-23 (NCEI Accession 0150873)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0150873 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  10. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Bell M. Shimada in the North Pacific Ocean from 2016-05-27 to 2016-05-28 (NCEI Accession 0164091)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0164091 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  11. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Bell M. Shimada in the North Pacific Ocean from 2016-03-23 to 2016-04-23 (NCEI Accession 0150875)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0150875 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  12. Underway meteorological, navigational, physical, profile and time series data collected aboard NOAA Ship Hi'ialakai in the North Pacific Ocean and Philippine Sea from 2014-03-05 to 2014-06-02 (NODC Accession 0119156)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0119156 contains raw underway meteorological, navigational, physical, profile and time series data logged by the Scientific Computer System (SCS)...

  13. Underway meteorological, navigational, physical and time series data collected aboard NOAA Ship Hi'ialakai in the North Pacific Ocean from 2015-07-09 to 2015-07-16 (NCEI Accession 0129903)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0129903 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...

  14. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Reuben Lasker in the North Pacific Ocean from 2016-04-26 to 2016-05-19 (NCEI Accession 0153493)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0153493 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  15. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Okeanos Explorer in the North Atlantic Ocean from 2014-02-08 to 2014-02-10 (NODC Accession 0123613)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0123613 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  16. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Pisces in the North Atlantic Ocean from 2013-07-01 to 2013-07-12 (NODC Accession 0117838)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0117838 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  17. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Reuben Lasker in the North Pacific Ocean from 2016-10-06 to 2016-10-13 (NCEI Accession 0164089)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0164089 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  18. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Pisces in the Gulf of Mexico and North Atlantic Ocean from 2013-04-05 to 2013-06-07 (NODC Accession 0117812)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0117812 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  19. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Bell M. Shimada in the North Pacific Ocean from 2016-04-28 to 2016-05-09 (NCEI Accession 0151241)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0151241 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  20. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Pisces in the Gulf of Mexico and North Atlantic Ocean from 2015-05-12 to 2015-06-10 (NCEI Accession 0129440)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0129440 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  1. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Bell M. Shimada in the North Pacific Ocean from 2017-05-15 to 2017-05-24 (NCEI Accession 0164430)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0164430 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  2. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Bell M. Shimada in the North Pacific Ocean from 2015-01-31 to 2015-02-04 (NODC Accession 0125756)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0125756 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  3. Underway meteorological, navigational, physical, profile and time series data collected aboard NOAA Ship Henry B. Bigelow in the Bay of Fundy and North Atlantic Ocean from 2013-09-06 to 2013-11-19 (NCEI Accession 0115901)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115901 contains raw underway meteorological, navigational, physical, profile and time series data logged by the Scientific Computer System (SCS)...

  4. Underway meteorological, navigational, physical and time series data collected aboard NOAA Ship Hi'ialakai in the North Pacific Ocean from 2013-06-26 to 2013-07-03 (NODC Accession 0099244)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0099244 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...

  5. Underway meteorological, navigational, physical and time series data collected aboard NOAA Ship Nancy Foster in the North Atlantic Ocean from 2014-05-19 to 2014-05-20 (NODC Accession 0118685)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0118685 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...

  6. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Pisces in the North Atlantic Ocean from 2016-10-18 to 2016-10-20 (NCEI Accession 0164092)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0164092 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  7. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Pisces in the North Atlantic Ocean from 2016-09-21 to 2016-09-29 (NCEI Accession 0164083)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0164083 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  8. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Pisces in the North Atlantic Ocean from 2016-11-12 to 2016-11-18 (NCEI Accession 0164093)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0164093 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  9. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Pisces in the North Atlantic Ocean from 2016-07-03 to 2016-08-03 (NCEI Accession 0155990)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0155990 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  10. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Pisces in the Caribbean Sea, Gulf of Mexico and North Atlantic Ocean from 2015-10-12 to 2015-11-24 (NCEI Accession 0138341)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0138341 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  11. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Pisces in the North Atlantic Ocean from 2016-10-04 to 2016-10-13 (NCEI Accession 0164086)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0164086 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  12. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Pisces in the Gulf of Mexico and North Atlantic Ocean from 2017-04-11 to 2017-06-17 (NCEI Accession 0164341)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0164341 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  13. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Pisces in the North Atlantic Ocean from 2017-06-20 to 2017-07-06 (NCEI Accession 0165349)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0165349 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  14. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Pisces in the North Atlantic Ocean from 2017-07-08 to 2017-07-26 (NCEI Accession 0165226)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0165226 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  15. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Gordon Gunter in the Gulf of Mexico from 2016-03-17 to 2016-03-22 (NCEI Accession 0150824)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0150824 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  16. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Gordon Gunter in the Gulf of Mexico and North Atlantic Ocean from 2016-04-10 to 2016-04-20 (NCEI Accession 0165360)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0165360 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  17. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Pisces in the Gulf of Mexico from 2015-08-24 to 2015-09-10 (NCEI Accession 0132045)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0132045 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  18. Underway meteorological, navigational, physical, profile and time series data collected aboard NOAA Ship Ronald H. Brown in the North Pacific Ocean and South Pacific Ocean from 2014-11-05 to 2014-11-24 (NODC Accession 0123338)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0123338 contains raw underway meteorological, navigational, physical, profile and time series data logged by the Scientific Computer System (SCS)...

  19. Underway meteorological, navigational, physical, profile and time series data collected aboard NOAA Ship Ronald H. Brown in the Arctic Ocean and North Pacific Ocean from 2015-01-14 to 2015-02-13 (NODC Accession 0126056)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0126056 contains raw underway meteorological, navigational, physical, profile and time series data logged by the Scientific Computer System (SCS)...

  20. Underway meteorological, navigational, physical, profile and time series data collected aboard NOAA Ship Ronald H. Brown in the North Pacific Ocean and South Pacific Ocean from 2015-02-27 to 2015-03-30 (NODC Accession 0127092)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0127092 contains raw underway meteorological, navigational, physical, profile and time series data logged by the Scientific Computer System (SCS)...

  1. Underway meteorological, navigational, physical and time series data collected aboard NOAA Ship Ronald H. Brown in the North Atlantic Ocean, Rio de la Plata and others from 2017-02-11 to 2017-03-15 (NCEI Accession 0164157)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0164157 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...

  2. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Oscar Dyson in the Bering Sea, Gulf of Alaska and North Pacific Ocean from 2013-06-08 to 2013-08-09 (NODC Accession 0123940)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0123940 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  3. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Oregon II in the Gulf of Mexico from 2015-05-01 to 2015-05-31 (NCEI Accession 0129419)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0129419 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  4. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Oscar Dyson in the Bering Sea from 2014-06-12 to 2014-08-14 (NODC Accession 0124303)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0124303 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  5. Underway meteorological, navigational, physical, profile and time series data collected aboard NOAA Ship Henry B. Bigelow in the North Atlantic Ocean from 2012-08-07 to 2012-08-24 (NODC Accession 0125711)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0125711 contains raw underway meteorological, navigational, physical, profile and time series data logged by the Scientific Computer System (SCS)...

  6. Underway meteorological, navigational, physical, profile and time series data collected aboard NOAA Ship Henry B. Bigelow in the North Atlantic Ocean from 2013-07-01 to 2013-08-18 (NODC Accession 0115902)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115902 contains raw underway meteorological, navigational, physical, profile and time series data logged by the Scientific Computer System (SCS)...

  7. Underway meteorological, navigational, physical and time series data collected aboard NOAA Ship Hi'ialakai in the North Pacific Ocean from 2015-07-27 to 2015-08-27 (NCEI Accession 0133933)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0133933 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...

  8. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Bell M. Shimada in the North Pacific Ocean from 2016-05-14 to 2016-05-28 (NCEI Accession 0164090)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0164090 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  9. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Gordon Gunter in the Gulf of Mexico and North Atlantic Ocean from 2017-07-02 to 2017-07-18 (NCEI Accession 0165352)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0165352 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  10. Underway meteorological, navigational, physical and time series data collected aboard NOAA Ship Fairweather in the Coastal Waters of SE Alaska and North Pacific Ocean from 2015-09-18 to 2015-11-13 (NCEI Accession 0137857)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0137857 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...

  11. Underway meteorological, navigational, physical and time series data collected aboard NOAA Ship Hi'ialakai in the North Pacific Ocean, Philippine Sea and South Pacific Ocean from 2017-03-26 to 2017-06-21 (NCEI Accession 0164429)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0164429 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...

  12. Underway meteorological, navigational, physical, profile and time series data collected aboard NOAA Ship Henry B. Bigelow in the North Atlantic Ocean from 2015-08-12 to 2015-08-21 (NCEI Accession 0131861)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0131861 contains raw underway meteorological, navigational, physical, profile and time series data logged by the Scientific Computer System (SCS)...

  13. Underway meteorological, navigational, physical and time series data collected aboard NOAA Ship Rainier in the Gulf of Alaska from 2014-06-04 to 2014-06-20 (NCEI Accession 0141106)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0141106 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...

  14. Underway meteorological, navigational, physical and time series data collected aboard NOAA Ship Thomas Jefferson in the North Atlantic Ocean from 2014-04-04 to 2014-11-18 (NODC Accession 0122407)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0122407 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...

  15. Underway meteorological, navigational, physical and time series data collected aboard NOAA Ship Okeanos Explorer in the North Atlantic Ocean from 2015-02-09 to 2015-02-13 (NODC Accession 0125757)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0125757 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...

  16. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Oscar Elton Sette in the North Pacific Ocean from 2017-03-29 to 2017-04-07 (NCEI Accession 0164431)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0164431 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  17. Underway meteorological, navigational, physical and time series data collected aboard NOAA Ship Okeanos Explorer in the North Pacific Ocean from 2015-09-12 to 2015-09-30 (NCEI Accession 0142173)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0142173 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...

  18. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Oscar Elton Sette in the North Pacific Ocean and Philippine Sea from 2015-06-11 to 2015-07-14 (NCEI Accession 0129902)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0129902 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  19. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Oregon II in the Gulf of Mexico from 2016-03-31 to 2016-04-22 (NCEI Accession 0150823)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0150823 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  20. Underway meteorological, navigational, physical and time series data collected aboard NOAA Ship Rainier in the Gulf of Alaska from 2015-09-16 to 2015-09-25 (NCEI Accession 0138191)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0138191 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...

  1. Underway meteorological, navigational, physical and time series data collected aboard NOAA Ship Rainier in the Gulf of Alaska from 2014-05-19 to 2014-09-04 (NODC Accession 0123694)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0123694 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...

  2. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Oscar Dyson in the Bering Sea from 2016-05-03 to 2016-05-04 (NCEI Accession 0165030)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0165030 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  3. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Oregon II in the Gulf of Mexico and North Atlantic Ocean from 2014-05-04 to 2014-05-31 (NODC Accession 0118842)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0118842 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  4. Underway meteorological, navigational, physical and time series data collected aboard NOAA Ship Fairweather in the Gulf of Alaska from 2014-04-28 to 2014-07-28 (NODC Accession 0126498)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0126498 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...

  5. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Gordon Gunter in the North Atlantic Ocean from 2014-05-05 to 2014-05-09 (NCEI Accession 0149716)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0149716 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  6. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Oscar Dyson in the Bering Sea from 2016-05-18 to 2016-06-08 (NCEI Accession 0165363)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0165363 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  7. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Oscar Dyson in the Gulf of Alaska and North Pacific Ocean from 2014-02-21 to 2014-03-01 (NODC Accession 0125086)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0125086 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  8. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Oscar Dyson in the Bering Sea, Gulf of Alaska and North Pacific Ocean from 2016-03-02 to 2016-03-09 (NCEI Accession 0150732)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0150732 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  9. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Oscar Dyson in the Gulf of Alaska from 2015-03-14 to 2015-03-31 (NCEI Accession 0130691)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0130691 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  10. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Oscar Dyson in the Bering Sea from 2015-09-06 to 2015-09-18 (NCEI Accession 0137412)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0137412 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  11. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Oscar Dyson in the Coastal Waters of SE Alaska from 2016-01-30 to 2016-02-01 (NCEI Accession 0150695)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0150695 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  12. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Oscar Dyson in the Bering Sea, Gulf of Alaska and North Pacific Ocean from 2015-05-14 to 2015-06-05 (NCEI Accession 0130586)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0130586 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  13. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Oscar Dyson in the Bering Sea, Gulf of Alaska and North Pacific Ocean from 2014-03-13 to 2014-03-25 (NODC Accession 0124597)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0124597 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  14. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Oscar Dyson in the Bering Sea from 2014-05-20 to 2014-06-08 (NODC Accession 0125267)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0125267 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  15. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Oscar Dyson in the Bering Sea, Gulf of Alaska and North Pacific Ocean from 2013-08-15 to 2013-09-19 (NODC Accession 0123941)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0123941 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  16. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Oscar Dyson in the Bering Sea, Gulf of Alaska and North Pacific Ocean from 2014-08-17 to 2014-10-06 (NODC Accession 0124596)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0124596 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  17. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Oscar Dyson in the Bering Sea, Gulf of Alaska and North Pacific Ocean from 2015-04-24 to 2015-05-10 (NCEI Accession 0130737)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0130737 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  18. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Gordon Gunter in the Gulf of Mexico and North Atlantic Ocean from 2015-04-14 to 2015-06-13 (NCEI Accession 0128347)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0128347 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  19. Underway meteorological, time series, navigational, physical and optical data collected aboard NOAA Ship Okeanos Explorer in the Gulf of Mexico and North Atlantic Ocean from 2014-02-24 to 2014-03-18 (NCEI Accession 0123616)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0123616 contains raw underway meteorological, time series, navigational, physical and optical data logged by the Scientific Computer System (SCS)...

  20. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Gordon Gunter in the North Atlantic Ocean from 2017-06-10 to 2017-06-23 (NCEI Accession 0164441)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0164441 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  1. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Gordon Gunter in the North Atlantic Ocean from 2016-05-21 to 2016-06-04 (NCEI Accession 0155169)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0155169 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  2. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Gordon Gunter in the Gulf of Mexico and North Atlantic Ocean from 2014-07-04 to 2014-07-31 (NODC Accession 0120740)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0120740 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  3. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Nancy Foster in the Gulf of Mexico and North Atlantic Ocean from 2015-06-15 to 2015-06-28 (NCEI Accession 0129875)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0129875 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  4. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Gordon Gunter in the Gulf of Mexico from 2016-09-16 to 2016-09-18 (NCEI Accession 0164081)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0164081 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  5. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Gordon Gunter in the Gulf of Mexico from 2016-10-31 to 2016-11-03 (NCEI Accession 0164446)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0164446 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  6. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Oscar Dyson in the Gulf of Alaska from 2017-03-14 to 2017-03-27 (NCEI Accession 0165012)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0165012 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  7. Underway meteorological, navigational, physical and time series data collected aboard NOAA Ship Okeanos Explorer in the North Pacific Ocean from 2015-10-07 to 2015-10-16 (NCEI Accession 0150689)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0150689 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...

  8. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Reuben Lasker in the North Pacific Ocean from 2017-03-21 to 2017-04-22 (NCEI Accession 0164340)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0164340 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  9. Underway meteorological, navigational, physical and time series data collected aboard NOAA Ship Okeanos Explorer in the North Atlantic Ocean from 2014-08-09 to 2014-10-07 (NODC Accession 0125346)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0125346 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...

  10. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Reuben Lasker in the North Pacific Ocean from 2016-01-06 to 2016-01-30 (NCEI Accession 0150692)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0150692 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  11. Underway meteorological, navigational, physical and time series data collected aboard NOAA Ship Ronald H. Brown in the Coastal Waters of SE Alaska and North Pacific Ocean from 2016-06-23 to 2016-07-09 (NCEI Accession 0155758)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0155758 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...

  12. Underway meteorological, navigational, physical and time series data collected aboard NOAA Ship Ronald H. Brown in the North Pacific Ocean from 2016-03-29 to 2016-04-25 (NCEI Accession 0155759)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0155759 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...

  13. Underway meteorological, navigational, physical and time series data collected aboard NOAA Ship Hi'ialakai in the North Pacific Ocean and South Pacific Ocean from 2015-01-22 to 2015-05-04 (NCEI Accession 0127322)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0127322 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...

  14. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Pisces in the North Atlantic Ocean from 2015-06-18 to 2015-07-01 (NCEI Accession 0129541)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0129541 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  15. Underway meteorological, navigational, physical and time series data collected aboard NOAA Ship Hi'ialakai in the North Pacific Ocean from 2013-07-09 to 2013-07-16 (NODC Accession 0113243)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113243 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...

  16. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Oscar Dyson in the Bering Sea, Gulf of Alaska and North Pacific Ocean from 2014-04-04 to 2014-05-02 (NODC Accession 0125006)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0125006 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  17. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Oregon II in the Gulf of Mexico from 2014-06-07 to 2014-07-19 (NODC Accession 0120616)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0120616 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  18. Underway meteorological, navigational, physical and time series data collected aboard NOAA Ship Thomas Jefferson in the North Atlantic Ocean from 2013-09-24 to 2013-11-04 (NODC Accession 0123614)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0123614 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...

  19. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Bell M. Shimada in the North Pacific Ocean from 2015-03-07 to 2015-03-22 (NODC Accession 0126660)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0126660 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  20. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Bell M. Shimada in the North Pacific Ocean from 2015-05-05 to 2015-05-18 (NCEI Accession 0128172)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0128172 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  1. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Oregon II in the Gulf of Mexico and North Atlantic Ocean from 2015-07-25 to 2015-09-27 (NCEI Accession 0132051)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0132051 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  2. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Nancy Foster in the North Atlantic Ocean from 2013-07-12 to 2013-07-21 (NODC Accession 0113448)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113448 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  3. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Gordon Gunter in the North Atlantic Ocean from 2016-06-30 to 2016-07-16 (NCEI Accession 0165361)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0165361 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  4. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Oscar Elton Sette in the North Pacific Ocean from 2014-09-25 to 2014-10-27 (NODC Accession 0123056)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0123056 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  5. Underway meteorological, navigational, physical, profile and time series data collected aboard NOAA Ship Henry B. Bigelow in the North Atlantic Ocean from 2015-05-19 to 2015-06-03 (NCEI Accession 0129421)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0129421 contains raw underway meteorological, navigational, physical, profile and time series data logged by the Scientific Computer System (SCS)...

  6. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Oregon II in the Gulf of Mexico and North Atlantic Ocean from 2014-07-26 to 2014-09-29 (NODC Accession 0122397)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0122397 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  7. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Oscar Elton Sette in the North Pacific Ocean and Philippine Sea from 2014-06-19 to 2014-07-19 (NODC Accession 0123094)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0123094 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  8. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Oscar Elton Sette in the North Pacific Ocean from 2014-08-30 to 2014-09-19 (NCEI Accession 0123092)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0123092 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  9. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Bell M. Shimada in the North Pacific Ocean from 2017-04-27 to 2017-05-11 (NCEI Accession 0164342)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0164342 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  10. Underway meteorological, navigational, physical and time series data collected aboard NOAA Ship Thomas Jefferson in the North Atlantic Ocean from 2017-07-11 to 2017-07-22 (NCEI Accession 0164798)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0164798 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...

  11. Underway meteorological, navigational, physical and time series data collected aboard NOAA Ship Thomas Jefferson in the North Atlantic Ocean from 2017-07-22 to 2017-07-26 (NCEI Accession 0164960)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0164960 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...

  12. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Oscar Elton Sette in the North Pacific Ocean from 2016-06-13 to 2016-06-22 (NCEI Accession 0155171)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0155171 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  13. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Oregon II in the Gulf of Mexico and North Atlantic Ocean from 2017-07-26 to 2017-08-10 (NCEI Accession 0164961)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0164961 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  14. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Hi'ialakai in the North Pacific Ocean from 2013-09-05 to 2013-09-20 (NODC Accession 0113248)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113248 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  15. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Bell M. Shimada in the North Pacific Ocean on 2016-06-26 (NCEI Accession 0162236)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0162236 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  16. Underway meteorological, navigational, physical, profile and time series data collected aboard NOAA Ship Henry B. Bigelow in the North Atlantic Ocean from 2014-07-24 to 2014-07-30 (NODC Accession 0125266)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0125266 contains raw underway meteorological, navigational, physical, profile and time series data logged by the Scientific Computer System (SCS)...

  17. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Gordon Gunter in the Gulf of Mexico from 2016-09-02 to 2016-10-01 (NCEI Accession 0164082)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0164082 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  18. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Oregon II in the Gulf of Mexico from 2017-06-05 to 2017-06-07 (NCEI Accession 0164786)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0164786 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  19. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Oscar Elton Sette in the North Pacific Ocean from 2015-04-03 to 2015-04-15 (NCEI Accession 0130368)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0130368 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  20. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Gordon Gunter in the Gulf of Mexico from 2012-10-19 to 2012-10-29 (NODC Accession 0113519)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113519 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  1. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Gordon Gunter in the Gulf of Mexico from 2016-10-23 to 2016-11-22 (NCEI Accession 0164155)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0164155 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  2. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Nancy Foster in the North Atlantic Ocean from 2013-08-31 to 2013-09-07 (NODC Accession 0113487)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113487 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  3. Underway meteorological, navigational, optical, physical, time series and trawl data collected aboard NOAA Ship Bell M. Shimada in the North Pacific Ocean from 2013-04-06 to 2013-04-30 (NCEI Accession 0115912)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115912 contains raw underway meteorological, navigational, optical, physical, time series and trawl data logged by the Scientific Computer System...

  4. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Bell M. Shimada in the North Pacific Ocean from 2016-01-09 to 2016-02-09 (NCEI Accession 0150817)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0150817 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  5. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Bell M. Shimada in the North Pacific Ocean from 2017-03-29 to 2017-04-20 (NCEI Accession 0164320)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0164320 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  6. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Bell M. Shimada in the North Pacific Ocean from 2015-10-06 to 2015-10-13 (NCEI Accession 0164861)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0164861 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  7. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Pisces in the North Atlantic Ocean from 2014-07-06 to 2014-08-02 (NODC Accession 0121197)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0121197 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  8. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Bell M. Shimada in the North Pacific Ocean from 2014-04-15 to 2014-05-12 (NODC Accession 0118545)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0118545 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  9. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Pisces in the North Atlantic Ocean from 2015-07-10 to 2015-08-04 (NCEI Accession 0130690)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0130690 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  10. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Bell M. Shimada in the Coastal Waters of SE Alaska and North Pacific Ocean from 2016-02-21 to 2016-03-11 (NCEI Accession 0150967)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0150967 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  11. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Oscar Dyson in the Gulf of Alaska and North Pacific Ocean from 2013-02-08 to 2013-03-05 (NODC Accession 0124184)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0124184 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  12. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Oscar Dyson in the Gulf of Alaska from 2017-03-01 to 2017-03-10 (NCEI Accession 0165011)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0165011 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  13. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Oscar Dyson in the Gulf of Alaska from 2013-03-14 to 2013-03-28 (NODC Accession 0124186)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0124186 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  14. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Oscar Dyson in the Gulf of Alaska and North Pacific Ocean from 2017-02-07 to 2017-02-16 (NCEI Accession 0164963)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0164963 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  15. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Oscar Dyson in the Bering Sea from 2016-08-22 to 2016-09-20 (NCEI Accession 0165088)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0165088 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  16. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Oscar Dyson in the Bering Sea, Gulf of Alaska and North Pacific Ocean from 2013-05-15 to 2013-06-01 (NODC Accession 0124207)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0124207 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  17. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Oscar Dyson in the Bering Sea, Gulf of Alaska and North Pacific Ocean from 2013-04-29 to 2013-05-11 (NODC Accession 0124208)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0124208 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  18. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Oscar Dyson in the Bering Sea from 2014-05-06 to 2014-05-17 (NODC Accession 0125087)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0125087 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  19. Underway meteorological, navigational, physical and time series data collected aboard NOAA Ship Oscar Dyson in the Coastal Waters of Southeast Alaska and British Columbia from 2016-10-18 to 2016-10-19 (NCEI Accession 0165092)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0165092 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...

  20. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Oscar Dyson in the Bering Sea, Gulf of Alaska and North Pacific Ocean from 2017-05-11 to 2017-06-02 (NCEI Accession 0165021)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0165021 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  1. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Oscar Dyson in the Bering Sea, Gulf of Alaska and North Pacific Ocean from 2015-08-20 to 2015-09-02 (NCEI Accession 0131578)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0131578 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  2. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Oscar Dyson in the Bering Sea, Gulf of Alaska and North Pacific Ocean from 2017-06-08 to 2017-07-15 (NCEI Accession 0165028)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0165028 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  3. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Oscar Dyson in the Bering Sea, Gulf of Alaska and North Pacific Ocean from 2015-09-23 to 2015-10-06 (NCEI Accession 0137392)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0137392 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  4. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Oscar Dyson in the Gulf of Alaska and North Pacific Ocean from 2015-02-09 to 2015-03-03 (NODC Accession 0127242)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0127242 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  5. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Oscar Dyson in the Bering Sea, Gulf of Alaska and North Pacific Ocean from 2016-09-30 to 2016-10-07 (NCEI Accession 0165090)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0165090 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  6. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Oscar Dyson in the Bering Sea from 2016-09-24 to 2016-10-01 (NCEI Accession 0165089)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0165089 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  7. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Oscar Dyson in the Coastal Waters of Southeast Alaska and British Columbia from 2016-01-25 to 2016-01-27 (NCEI Accession 0164857)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0164857 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  8. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Oscar Dyson in the Bering Sea, Gulf of Alaska and North Pacific Ocean from 2016-03-09 to 2016-03-24 (NCEI Accession 0150822)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0150822 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  9. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Oscar Dyson in the Bering Sea from 2016-06-12 to 2016-08-17 (NCEI Accession 0165031)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0165031 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  10. Underway meteorological, navigational, physical, profile and time series data collected aboard NOAA Ship Henry B. Bigelow in the North Atlantic Ocean from 2012-05-31 to 2012-06-14 (NODC Accession 0125709)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0125709 contains raw underway meteorological, navigational, physical, profile and time series data logged by the Scientific Computer System (SCS)...

  11. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Oscar Dyson in the Gulf of Alaska and North Pacific Ocean from 2016-02-11 to 2016-02-20 (NCEI Accession 0150731)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0150731 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  12. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Bell M. Shimada in the North Pacific Ocean from 2015-05-29 to 2015-06-10 (NCEI Accession 0129494)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0129494 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  13. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Pisces in the Gulf of Mexico from 2016-05-26 to 2016-05-31 (NCEI Accession 0155295)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0155295 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  14. Underway meteorological, navigational, optical and physical data collected aboard NOAA Ship Oscar Elton Sette in the North Pacific Ocean from 2013-09-11 to 2013-09-30 (NODC Accession 0117012)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0117012 contains raw underway meteorological, navigational, optical and physical data logged by the Scientific Computer System (SCS) aboard NOAA Ship...

  15. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Oscar Dyson in the Bering Sea, Gulf of Alaska and North Pacific Ocean from 2017-04-22 to 2017-05-08 (NCEI Accession 0165013)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0165013 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  16. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Oscar Elton Sette in the North Pacific Ocean and South Pacific Ocean from 2016-02-21 to 2016-03-25 (NCEI Accession 0155172)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0155172 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  17. Underway meteorological, navigational, physical, profile and time series data collected aboard NOAA Ship Hi'ialakai in the North Pacific Ocean and Philippine Sea from 2014-05-11 to 2014-05-22 (NODC Accession 0119200)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0119200 contains raw underway meteorological, navigational, physical, profile and time series data logged by the Scientific Computer System (SCS)...

  18. Underway meteorological, navigational, physical and time series data collected aboard NOAA Ship Fairweather in the Coastal Waters of Southeast Alaska and British Columbia and North Pacific Ocean from 2016-05-25 to 2016-06-18 (NCEI Accession 0162234)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0162234 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...

  19. Underway meteorological, navigational, physical and time series data collected aboard NOAA Ship Nancy Foster in the North Atlantic Ocean from 2014-04-20 to 2014-04-30 (NODC Accession 0118187)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0118187 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...

  20. Underway meteorological, navigational, physical and time series data collected aboard NOAA Ship Hi'ialakai in the North Pacific Ocean from 2014-09-06 to 2014-09-30 (NODC Accession 0122499)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0122499 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...

  1. Underway meteorological, navigational, physical and time series data collected aboard NOAA Ship Henry B. Bigelow in the North Atlantic Ocean from 2017-07-06 to 2017-07-19 (NCEI Accession 0164783)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0164783 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...

  2. Underway meteorological, navigational, physical, profile and time series data collected aboard NOAA Ship Henry B. Bigelow in the North Atlantic Ocean from 2012-02-27 to 2012-05-04 (NODC Accession 0125710)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0125710 contains raw underway meteorological, navigational, physical, profile and time series data logged by the Scientific Computer System (SCS)...

  3. Underway meteorological, navigational, physical, profile and time series data collected aboard NOAA Ship Henry B. Bigelow in the North Atlantic Ocean from 2015-07-27 to 2015-08-07 (NCEI Accession 0130538)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0130538 contains raw underway meteorological, navigational, physical, profile and time series data logged by the Scientific Computer System (SCS)...

  4. Underway meteorological, navigational, physical, profile and time series data collected aboard NOAA Ship Henry B. Bigelow in the North Atlantic Ocean from 2014-08-05 to 2014-08-16 (NODC Accession 0125265)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0125265 contains raw underway meteorological, navigational, physical, profile and time series data logged by the Scientific Computer System (SCS)...

  5. Underway meteorological, navigational, physical and time series data collected aboard NOAA Ship Nancy Foster in the Gulf of Mexico and North Atlantic Ocean from 2014-09-01 to 2014-09-14 (NODC Accession 0123337)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0123337 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...

  6. Underway meteorological, navigational, physical, profile and time series data collected aboard NOAA Ship Ronald H. Brown in the Coral Sea, North Pacific Ocean and South Pacific Ocean from 2014-10-06 to 2014-11-01 (NODC Accession 0123096)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0123096 contains raw underway meteorological, navigational, physical, profile and time series data logged by the Scientific Computer System (SCS)...

  7. Underway meteorological, navigational, physical, profile and time series data collected aboard NOAA Ship Henry B. Bigelow in the North Atlantic Ocean from 2017-06-08 to 2017-06-22 (NCEI Accession 0164795)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0164795 contains raw underway meteorological, navigational, physical, profile and time series data logged by the Scientific Computer System (SCS)...

  8. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Oscar Elton Sette in the North Pacific Ocean and Philippine Sea from 2014-07-24 to 2014-08-25 (NODC Accession 0123095)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0123095 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  9. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Oscar Elton Sette in the North Pacific Ocean and Philippine Sea from 2014-05-31 to 2014-06-16 (NODC Accession 0123093)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0123093 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  10. Underway meteorological, navigational, physical and time series data collected aboard NOAA Ship Henry B. Bigelow in the North Atlantic Ocean from 2015-11-12 to 2015-11-17 (NCEI Accession 0138157)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0138157 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...

  11. Underway navigational, physical and time series data collected aboard NOAA Ship Thomas Jefferson in the North Atlantic Ocean from 2013-06-17 to 2013-10-02 (NODC Accession 0123055)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0123055 contains raw underway navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA Ship Thomas...

  12. Underway meteorological, navigational, physical, profile and time series data collected aboard NOAA Ship Henry B. Bigelow in the North Atlantic Ocean from 2015-05-19 to 2015-06-03 (NCEI Accession 0134847)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0134847 contains raw underway meteorological, navigational, physical, profile and time series data logged by the Scientific Computer System (SCS)...

  13. Underway meteorological, navigational, physical, profile and time series data collected aboard NOAA Ship Henry B. Bigelow in the North Atlantic Ocean from 2014-06-18 to 2014-07-01 (NODC Accession 0125584)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0125584 contains raw underway meteorological, navigational, physical, profile and time series data logged by the Scientific Computer System (SCS)...

  14. Underway meteorological, navigational, physical, profile and time series data collected aboard NOAA Ship Henry B. Bigelow in the North Atlantic Ocean from 2013-06-10 to 2013-06-24 (NODC Accession 0115702)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115702 contains raw underway meteorological, navigational, physical, profile and time series data logged by the Scientific Computer System (SCS)...

  15. Underway meteorological, navigational, physical, profile and time series data collected aboard NOAA Ship Henry B. Bigelow in the North Atlantic Ocean from 2012-06-18 to 2012-06-28 (NODC Accession 0125666)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0125666 contains raw underway meteorological, navigational, physical, profile and time series data logged by the Scientific Computer System (SCS)...

  16. Underway meteorological, navigational, physical and time series data collected aboard NOAA Ship Thomas Jefferson in the North Atlantic Ocean from 2013-03-11 to 2013-03-13 (NODC Accession 0123054)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0123054 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...

  17. Underway meteorological, navigational, physical, profile and time series data collected aboard NOAA Ship Henry B. Bigelow in the North Atlantic Ocean from 2012-07-04 to 2012-07-18 (NODC Accession 0125758)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0125758 contains raw underway meteorological, navigational, physical, profile and time series data logged by the Scientific Computer System (SCS)...

  18. Underway meteorological, navigational, physical, profile and time series data collected aboard NOAA Ship Henry B. Bigelow in the North Atlantic Ocean from 2012-09-04 to 2012-11-11 (NODC Accession 0125919)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0125919 contains raw underway meteorological, navigational, physical, profile and time series data logged by the Scientific Computer System (SCS)...

  19. Underway meteorological, navigational, physical and time series data collected aboard NOAA Ship Ronald H. Brown in the North Pacific Ocean and South Pacific Ocean from 2014-08-25 to 2014-09-27 (NODC Accession 0122504)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0122504 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...

  20. Underway meteorological, navigational, physical, profile and time series data collected aboard NOAA Ship Henry B. Bigelow in the North Atlantic Ocean from 2016-07-18 to 2016-08-25 (NCEI Accession 0162239)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0162239 contains raw underway meteorological, navigational, physical, profile and time series data logged by the Scientific Computer System (SCS)...

  1. Underway meteorological, navigational, physical, profile and time series data collected aboard NOAA Ship Henry B. Bigelow in the North Atlantic Ocean from 2013-03-14 to 2013-05-09 (NODC Accession 0115052)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115052 contains raw underway meteorological, navigational, physical, profile and time series data logged by the Scientific Computer System (SCS)...

  2. Underway meteorological, navigational, physical, profile and time series data collected aboard NOAA Ship Henry B. Bigelow in the North Atlantic Ocean from 2015-06-10 to 2015-07-02 (NCEI Accession 0129527)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0129527 contains raw underway meteorological, navigational, physical, profile and time series data logged by the Scientific Computer System (SCS)...

  3. Underway meteorological, navigational, physical and time series data collected aboard NOAA Ship Thomas Jefferson in the North Atlantic Ocean from 2015-06-24 to 2015-07-03 (NCEI Accession 0142627)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0142627 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...

  4. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Pisces in the North Atlantic Ocean from 2016-06-08 to 2016-06-25 (NCEI Accession 0155294)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0155294 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  5. Underway meteorological, navigational, physical and time series data collected aboard NOAA Ship Nancy Foster in the North Atlantic Ocean from 2014-11-12 to 2014-11-21 (NODC Accession 0125582)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0125582 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...

  6. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Gordon Gunter in the Gulf of Mexico and North Atlantic Ocean from 2015-08-07 to 2015-09-28 (NCEI Accession 0131988)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0131988 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  7. Underway meteorological, navigational, physical and time series data collected aboard NOAA Ship Ronald H. Brown in the North Pacific Ocean and South Pacific Ocean from 2014-03-23 to 2014-04-08 (NODC Accession 0120490)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0120490 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...

  8. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Oregon II in the Gulf of Mexico from 2017-03-20 to 2017-04-20 (NCEI Accession 0164319)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0164319 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  9. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Nancy Foster in the Gulf of Mexico and North Atlantic Ocean from 2013-09-10 to 2013-09-20 (NODC Accession 0113488)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113488 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  10. Underway meteorological, navigational, physical and time series data collected aboard NOAA Ship Ronald H. Brown in the Gulf of Alaska, North Pacific Ocean and South Pacific Ocean from 2015-04-10 to 2015-06-24 (NCEI Accession 0129524)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0129524 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...

  11. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Pisces in the Gulf of Mexico from 2014-08-22 to 2014-09-12 (NODC Accession 0121982)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0121982 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  12. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Bell M. Shimada in the North Pacific Ocean from 2013-03-01 to 2013-03-10 (NODC Accession 0116096)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0116096 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  13. Underway meteorological, navigational, physical and time series data collected aboard NOAA Ship Ronald H. Brown in the Arctic Ocean, Beaufort Sea and others from 2015-08-06 to 2015-09-04 (NCEI Accession 0141104)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0141104 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...

  14. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Oregon II in the Gulf of Mexico from 2013-10-24 to 2013-11-22 (NODC Accession 0116135)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0116135 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  15. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Bell M. Shimada in the North Pacific Ocean from 2013-01-11 to 2013-02-02 (NCEI Accession 0115911)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115911 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  16. Underway meteorological, navigational, physical and time series data collected aboard NOAA Ship Okeanos Explorer in the Gulf of Mexico and North Atlantic Ocean from 2014-05-07 to 2014-05-22 (NODC Accession 0125618)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0125618 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...

  17. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Oregon II in the Gulf of Mexico from 2015-10-08 to 2015-11-21 (NCEI Accession 0138304)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0138304 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  18. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Bell M. Shimada in the North Pacific Ocean from 2014-09-25 to 2014-09-30 (NCEI Accession 0136936)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0136936 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  19. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Bell M. Shimada in the North Pacific Ocean from 2017-02-17 to 2017-02-25 (NCEI Accession 0164313)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0164313 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  20. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Oregon II in the Gulf of Mexico from 2016-04-29 to 2016-05-31 (NCEI Accession 0152488)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0152488 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  1. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Oregon II in the Gulf of Mexico from 2017-04-28 to 2017-05-30 (NCEI Accession 0164785)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0164785 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  2. Chromatographic monitoring procedures in laboratory practice

    Energy Technology Data Exchange (ETDEWEB)

    Kaplina, E G; Belova, O I; Lasunina, N A

    1976-01-01

    The Moscow Coke and Chemical Works consist of three plants in combination, viz., the coking plant, the synthetic ammonia plant using coke-oven-gas hydrogen and the oxygen plant. The plant requirements include daily analyses not only of the coke-oven gas but also of a rich gas and an ethylene fraction. The analyses are carried out in VTI-2 apparatus. The analytical data are used to calculate the calorific values and densities of the gases. The time requirements are very considerable and the laboratory has long been engaged in developing and introducing chromatographic procedures for the major constituents of coke-oven gas, rich gas and ethylene fraction. The procedure developed for the coke-oven and rich gases uses two parallel columns, one packed with molecular sieves and the other with grade KSM silica gel. Hydrogen was determined with argon as the carrier gas, and all other constituents with helium. The procedure was time-consuming and complicated. An attempt was made to separate the gases in an LKhM-7a chromatograph with a programme-controlled 50 to 250/sup 0/C heating cycle, but the procedure still had a number of serious defects and could not be recommended for regular quality control. The final variant involved two parallel columns and a procedure based on that in GOST 14920 (''Dry gas. Proximate analysis''). The chromatograph was a type KhL-69 with a 6-way cock in the gas line so that each of the columns could be brought on stream in succession. The analytical column packings were zeolite (in a 2 m column) and diatomaceous brick with 25% n-hexadecane (in a 6 m column).

  3. Finally Underway: Implementation of the Port Hope Area Initiative - 13151

    International Nuclear Information System (INIS)

    Fahey, Christine A.; Palmeter, Tim; Blanchette, Marcia

    2013-01-01

    Two distinct yet closely related waste remediation projects are finally underway in Canada under the Port Hope Area Initiative (PHAI) which aims to clean up 1.7 million cubic metres (m 3 ) of low-level radioactive waste (LLRW) arising from 60 years of uranium and radium operations. Under the PHAI, the Port Hope Project and the smaller Port Granby Project will result in the consolidation of the LLRW within two highly engineered, above-ground mounds, to be constructed within the municipalities of Port Hope and Clarington. These projects will fulfill the federal government commitment to the safe, long-term management of the LLRW, as set out in the legal agreement signed by the government and the host municipalities in 2001. The federal authorization to commence PHAI Remediation and Construction Phase 2 was received in late 2011 and several enabling infrastructure construction and radiological survey contracts were awarded in 2012. The contracts to remediate the waste sites and construct the new engineered mounds will be tendered in 2013. At the end of Phase 2, environmental risks will be substantially mitigated, land development restrictions lifted, and an honourable legacy left for future generations. (authors)

  4. Visualized Characterization for Cerebral Response of Acupuncture Deqi: Paradox Underway

    Directory of Open Access Journals (Sweden)

    Jie Yang

    2013-01-01

    Full Text Available Acupuncture as an oriental natural healing therapy with prolonged history has been extensively utilized in the management of great numbers of disorders. Deqi, a renowned acupuncture needling sensation, is profoundly regarded as the predictor and also the prerequisite of a preferable acupuncture treatment efficacy. Till now, there is still no consistency being reached towards the mechanism of acupuncture Deqi as a result of the discrepancy for publicly acknowledged evidence. Recent visualized research on Deqi using modern technologies has demonstrated possible central mechanism towards it. However, there is a conspicuous paradox underway in the research of cerebral response to acupuncture Deqi. This paper provided a view of up-to-date studies using visualized tools to characterize the brain response to acupuncture Deqi, such as functional magnetic resonance imaging (fMRI and positron emission tomography/computed tomography (PET/CT. The paradox was extruded to highlight certain reasons from a TCM view. It is hypothesized that acupoints located at different dermal sites, state of participant, and needling manipulation can all contribute to the current paradox. Hence, further studies on acupuncture Deqi should pay more attention to the strategy of experiment design with generalized measurement, valid sham control methods, and more to subjects in diseased condition.

  5. Simple gas chromatographic method for furfural analysis.

    Science.gov (United States)

    Gaspar, Elvira M S M; Lopes, João F

    2009-04-03

    A new, simple, gas chromatographic method was developed for the direct analysis of 5-hydroxymethylfurfural (5-HMF), 2-furfural (2-F) and 5-methylfurfural (5-MF) in liquid and water soluble foods, using direct immersion SPME coupled to GC-FID and/or GC-TOF-MS. The fiber (DVB/CAR/PDMS) conditions were optimized: pH effect, temperature, adsorption and desorption times. The method is simple and accurate (RSDfurfurals will contribute to characterise and quantify their presence in the human diet.

  6. Improved Submariner Eyewear for Routine Wear and Emergency Equipment Use Underway

    Science.gov (United States)

    2010-01-15

    information. 2.0 DESCRIPTION Naval Submarine Medical Research Laboratory (NSMRL) is seeking information from the eyewear industry that will provide...Improved Submariner Eyewear for Routine Wear and Emergency Equipment Use Underway by Alison America, MA Wayne G. Horn, MD...Submariner Eyewear for Routine Wear and Emergency Equipment Use Underway 50818 Alison America, MA Wayne G. Horn, MD Naval Submarine Medical Research

  7. Hydrocarbon Fuel Thermal Performance Modeling based on Systematic Measurement and Comprehensive Chromatographic Analysis

    Science.gov (United States)

    2016-07-31

    distribution unlimited Hydrocarbon Fuel Thermal Performance Modeling based on Systematic Measurement and Comprehensive Chromatographic Analysis Matthew...vital importance for hydrocarbon -fueled propulsion systems: fuel thermal performance as indicated by physical and chemical effects of cooling passage... analysis . The selection and acquisition of a set of chemically diverse fuels is pivotal for a successful outcome since test method validation and

  8. High Performance Liquid Chromatographic Analysis of Phytoplankton Pigments Using a C16-Amide Column

    Science.gov (United States)

    A reverse-phase high performance liquid chromatographic (RP-HPLC) method was developed to analyze in a single run, most polar and non-polar chlorophylls and carotenoids from marine phytoplankton. The method is based on a RP-C16-Amide column and a ternary gradient system consistin...

  9. The combination of the liquid chromatograph with the nuclear magnetic resonance spectrometer

    International Nuclear Information System (INIS)

    Scott, R.P.W.

    1986-01-01

    The association of the liquid chromatograph with the NMR spectrometer would be a very powerful analytical system for the separation and identification of unknown mixtures. There are, however, some serious difficulties involved with the association of these two techniques. The historical development of NMR chromatography is outlined and some problems are discussed. (Auth.)

  10. High-Order Approximation of Chromatographic Models using a Nodal Discontinuous Galerkin Approach

    DEFF Research Database (Denmark)

    Meyer, Kristian; Huusom, Jakob Kjøbsted; Abildskov, Jens

    2018-01-01

    by Javeed et al. (2011a,b, 2013) with an efficient quadrature-free implementation. The framework is used to simulate linear and non-linear multicomponent chromatographic systems. The results confirm arbitrary high-order accuracy and demonstrate the potential for accuracy and speed-up gains obtainable...

  11. Modeling chromatographic columns. Non-equilibrium packed-bed adsorption with non-linear adsorption isotherms

    NARCIS (Netherlands)

    Özdural, A.R.; Alkan, A.; Kerkhof, P.J.A.M.

    2004-01-01

    In this work a new mathematical model, based on non-equilibrium conditions, describing the dynamic adsorption of proteins in columns packed with spherical adsorbent particles is used to study the performance of chromatographic systems. Simulations of frontal chromatography, including axial

  12. Supercritical Fluid Chromatographic Separation of Dimethylpolysiloxane Polymer

    Energy Technology Data Exchange (ETDEWEB)

    Pyo, Dong Jin; Lim, Chang Hyun [Kangwon National University, Chuncheon (Korea, Republic of)

    2005-02-15

    Water was used as a polar modifier and a μ-porasil column as a saturator column. The μ-porasil column was inserted between the pump outlet and the injection valve. During the passage of the supercritical fluid mobile phase through the silica column, a polar modifier (water) can be dissolved in the pressurized supercritical fluid. Dimethylpolysiloxane polymer has been known as more polar polymer than polystyrene polymer. Dimethylpolysiloxane polymer has never been separated using water modified mobile phase. In this paper, using a μ-porasil column as a saturator column, excellent supercritical fluid chromatograms of dimethylpolysiloxane oligomers were obtained. The use of compressed (dense) gases and supercritical fluids as chromatographic mobile phases in conjunction with liquid chromatographic (LC)-type packed columns was first reported by Klesper et al. in 1962. During its relatively short history, supercritical fluid chromatography (SFC) has become an attractive alternative to GC and LC in certain industrially important applications. SFC gives the advantage of high efficiency and allows the analysis of nonvolatile or thermally labile mixtures.

  13. Supercritical Fluid Chromatographic Separation of Dimethylpolysiloxane Polymer

    International Nuclear Information System (INIS)

    Pyo, Dong Jin; Lim, Chang Hyun

    2005-01-01

    Water was used as a polar modifier and a μ-porasil column as a saturator column. The μ-porasil column was inserted between the pump outlet and the injection valve. During the passage of the supercritical fluid mobile phase through the silica column, a polar modifier (water) can be dissolved in the pressurized supercritical fluid. Dimethylpolysiloxane polymer has been known as more polar polymer than polystyrene polymer. Dimethylpolysiloxane polymer has never been separated using water modified mobile phase. In this paper, using a μ-porasil column as a saturator column, excellent supercritical fluid chromatograms of dimethylpolysiloxane oligomers were obtained. The use of compressed (dense) gases and supercritical fluids as chromatographic mobile phases in conjunction with liquid chromatographic (LC)-type packed columns was first reported by Klesper et al. in 1962. During its relatively short history, supercritical fluid chromatography (SFC) has become an attractive alternative to GC and LC in certain industrially important applications. SFC gives the advantage of high efficiency and allows the analysis of nonvolatile or thermally labile mixtures

  14. The gas-chromatographic and gas-chromatographic-mass-spectrometric identification of halogen-containing organic compounds

    Science.gov (United States)

    Gidaspov, B. V.; Zenkevich, I. G.; Rodin, A. A.

    1989-09-01

    The problem of identifying halogen-containing organic compounds in their gas-chromatographic and gas-chromatographic-mass-spectrometric (GC-MS) determination in different materials has been examined. Particular attention has been paid not to the complete characterisation of methods for carrying out this analysis but to the most important problem of increasing the selectivity at the stages of sampling, separation, and interpretation of the gas-chromatographic and GC-MS information. The bibliography contains 292 references.

  15. Partial pressure (or fugacity) of carbon dioxide, temperature, salinity and other variables collected from surface underway observations using carbon dioxide gas analyzer, shower head equilibrator and other instruments from R/V Wecoma in the U.S. West Coast California Current System during the 2011 West Coast Ocean Acidification Cruise (WCOA2011) from 2011-08-12 to 2011-08-30 (NODC Accession 0123607)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This archival package contains the surface underway pCO2 data of the first dedicated West Coast Ocean Acidification cruise (WCOA2011). The cruise took place August...

  16. High-performance liquid chromatographic method for guanylhydrazone compounds.

    Science.gov (United States)

    Cerami, C; Zhang, X; Ulrich, P; Bianchi, M; Tracey, K J; Berger, B J

    1996-01-12

    A high-performance liquid chromatographic method has been developed for a series of aromatic guanylhydrazones that have demonstrated therapeutic potential as anti-inflammatory agents. The compounds were separated using octadecyl or diisopropyloctyl reversed-phase columns, with an acetonitrile gradient in water containing heptane sulfonate, tetramethylammonium chloride, and phosphoric acid. The method was used to reliably quantify levels of analyte as low as 785 ng/ml, and the detector response was linear to at least 50 micrograms/ml using a 100 microliters injection volume. The assay system was used to determine the basic pharmacokinetics of a lead compound, CNI-1493, from serum concentrations following a single intravenous injection in rats.

  17. Gas-Chromatographic Determination Of Water In Freon PCA

    Science.gov (United States)

    Melton, Donald M.

    1994-01-01

    Gas-chromatographic apparatus measures small concentrations of water in specimens of Freon PCA. Testing by use of apparatus faster and provides greater protection against accidental contamination of specimens by water in testing environment. Automated for unattended operation. Also used to measure water contents of materials, other than Freon PCA. Innovation extended to development of purgeable sampling accessory for gas chromatographs.

  18. Probabilistic peak detection for first-order chromatographic data

    NARCIS (Netherlands)

    Lopatka, M.; Vivó-Truyols, G.; Sjerps, M.J.

    2014-01-01

    We present a novel algorithm for probabilistic peak detection in first-order chromatographic data. Unlike conventional methods that deliver a binary answer pertaining to the expected presence or absence of a chromatographic peak, our method calculates the probability of a point being affected by

  19. Chromatographic and electrophoretic approaches in ink analysis.

    Science.gov (United States)

    Zlotnick, J A; Smith, F P

    1999-10-15

    Inks are manufactured from a wide variety of substances that exhibit very different chemical behaviors. Inks designed for use in different writing instruments or printing methods have quite dissimilar components. Since the 1950s chromatographic and electrophoretic methods have played important roles in the analysis of inks, where compositional information may have bearing on the investigation of counterfeiting, fraud, forgery, and other crimes. Techniques such as paper chromatography and electrophoresis, thin-layer chromatography, high-performance liquid chromatography, gas chromatography, gel electrophoresis, and the relatively new technique of capillary electrophoresis have all been explored as possible avenues for the separation of components of inks. This paper reviews the components of different types of inks and applications of the above separation methods are reviewed.

  20. New extraction chromatographic material for rhenium separation

    International Nuclear Information System (INIS)

    Lucanikova, M.; Czech Technical University, Prague; Kucera, J.; Czech Technical University, Prague; Sebesta, F.

    2008-01-01

    Three types of the extraction chromatographic materials, composed from Aliquat R 336 deposited in the polyacrylonitrile (PAN) beads and prepared by different procedures, were compared for extraction of rhenium. The best properties were exhibited when the solid extractant was prepared by impregnation of the ready-made PAN beads. Solid extractant prepared by direct coagulation of the beads from the suspension of Aliquat R 336 in solution of PAN in nitric acid differs only by lower capacity in dynamic conditions. Material prepared from the PAN solution in dimethylsulfoxide was the worst because Aliquat R 336 was washed out from the beads during coagulation of the polymer and the extraction capacity was low. As it is shown, the first two solid extractants are fully comparable with the commercial TEVA Resin. (author)

  1. Aligning of single and multiple wavelength chromatographic

    DEFF Research Database (Denmark)

    Nielsen, Niels-Peter Vest; Carstensen, Jens Michael; Smedsgaard, Jørn

    1998-01-01

    optimised warping (COW) using two input parameters which can be estimated from the observed peak width. COW is demonstrated on constructed single trace chromatograms and on single and multiple wavelength chromatograms obtained from HPLC diode detection analyses of fungal extractsA copy of the C program......The use of chemometric data processing is becoming an important part of modern chromatography. Most chemometric analyses are performed on reduced data sets using areas of selected peaks detected in the chromatograms, which means a loss of data and introduces the problem of extracting peak data from...... to utilise the entire data matrix or rely on peak detection, thus having the same limitations as the commonly used chemometric procedures. The method presented uses the entire chromatographic data matrices and does not require any preprocessing e.g., peak detection. It relies on piecewise linear correlation...

  2. High-performance liquid-chromatographic separation of subcomponents of antimycin-A

    Science.gov (United States)

    Abidi, S.L.

    1988-01-01

    Using a reversed-phase high-performance liquid chromatographic (HPLC) technique, a mixture of antimycins A was separated into eight hitherto unreported subcomponents, Ala, Alb, A2a, A2b, A3a, A3b, A4a, and A4b. Although a base-line resolution of the known four major antimycins Al, A2, A3, and A4 was readily achieved with mobile phases containing acetate buffers, the separation of the new antibiotic subcomponents was highly sensitive to variation in mobile phase conditions. The type and composition of organic modifiers, the nature of buffer salts, and the concentration of added electrolytes had profound effects on capacity factors, separation factors, and peak resolution values. Of the numerous chromatographic systems examined, a mobile phase consisting of methanol-water (70:30) and 0.005 M tetrabutylammonium phosphate at pH 3.0 yielded the most satisfactory results for the separation of the subcomponents. Reversed-phase gradient HPLC separation of the dansylated or methylated antibiotic compounds produced superior chromatographic characteristics and the presence of added electrolytes was not a critical factor for achieving separation. Differences in the chromatographic outcome between homologous and structural isomers were interpretated based on a differential solvophobic interaction rationale. Preparative reversed-phase HPLC under optimal conditions enabled isolation of pure samples of the methylated antimycin subcomponents for use in structural studies.

  3. Secretory immunoglobulin purification from whey by chromatographic techniques.

    Science.gov (United States)

    Matlschweiger, Alexander; Engelmaier, Hannah; Himmler, Gottfried; Hahn, Rainer

    2017-08-15

    Secretory immunoglobulins (SIg) are a major fraction of the mucosal immune system and represent potential drug candidates. So far, platform technologies for their purification do not exist. SIg from animal whey was used as a model to develop a simple, efficient and potentially generic chromatographic purification process. Several chromatographic stationary phases were tested. A combination of two anion-exchange steps resulted in the highest purity. The key step was the use of a small-porous anion exchanger operated in flow-through mode. Diffusion of SIg into the resin particles was significantly hindered, while the main impurities, IgG and serum albumin, were bound. In this step, initial purity was increased from 66% to 89% with a step yield of 88%. In a second anion-exchange step using giga-porous material, SIg was captured and purified by step or linear gradient elution to obtain fractions with purities >95%. For the step gradient elution step yield of highly pure SIg was 54%. Elution of SIgA and SIgM with a linear gradient resulted in a step yield of 56% and 35%, respectively. Overall yields for both anion exchange steps were 43% for the combination of flow-through and step elution mode. Combination of flow-through and linear gradient elution mode resulted in a yield of 44% for SIgA and 39% for SIgM. The proposed process allows the purification of biologically active SIg from animal whey in preparative scale. For future applications, the process can easily be adopted for purification of recombinant secretory immunoglobulin species. Copyright © 2017 Elsevier B.V. All rights reserved.

  4. Recent Advances in Water Analysis with Gas Chromatograph Mass Spectrometers

    Science.gov (United States)

    MacAskill, John A.; Tsikata, Edem

    2014-01-01

    We report on progress made in developing a water sampling system for detection and analysis of volatile organic compounds in water with a gas chromatograph mass spectrometer (GCMS). Two approaches are described herein. The first approach uses a custom water pre-concentrator for performing trap and purge of VOCs from water. The second approach uses a custom micro-volume, split-splitless injector that is compatible with air and water. These water sampling systems will enable a single GC-based instrument to analyze air and water samples for VOC content. As reduced mass, volume, and power is crucial for long-duration, manned space-exploration, these water sampling systems will demonstrate the ability of a GCMS to monitor both air and water quality of the astronaut environment, thereby reducing the amount of required instrumentation for long duration habitation. Laboratory prototypes of these water sampling systems have been constructed and tested with a quadrupole ion trap mass spectrometer as well as a thermal conductivity detector. Presented herein are details of these water sampling system with preliminary test results.

  5. Method for chromatographically recovering scandium and yttrium

    International Nuclear Information System (INIS)

    Snyder, T.S.; Stoltz, R.A.

    1991-01-01

    This paper describes a method for chromatographically recovering scandium and yttrium from the residue of a sand chlorinator. It comprises: providing a residue from a sand chlorinator, the residue containing scandium, yttrium, sodium, calcium and at least one radioactive metal of the group consisting of radium, thorium and uranium; digesting the residue with an acid to produce an aqueous liquid containing scandium, yttrium, sodium, calcium and at least one radioactive metal of the group consisting of radium, thorium and uranium; feeding the metal containing liquid through a cation exchanger; eluding the cation exchanger with an acid eluant to to produce: a first eluate containing at least half of the total weight of the calcium and sodium in the feed liquid; a second eluate containing at least half of the total weight of the one or more radioactive metals in the feed liquid; a third eluate containing at least half of the yttrium in the feed liquid, and a fourth eluate containing at least half of the weight of the scandium in the feed liquid

  6. Chromatographic Techniques for Rare Earth Elements Analysis

    Science.gov (United States)

    Chen, Beibei; He, Man; Zhang, Huashan; Jiang, Zucheng; Hu, Bin

    2017-04-01

    The present capability of rare earth element (REE) analysis has been achieved by the development of two instrumental techniques. The efficiency of spectroscopic methods was extraordinarily improved for the detection and determination of REE traces in various materials. On the other hand, the determination of REEs very often depends on the preconcentration and separation of REEs, and chromatographic techniques are very powerful tools for the separation of REEs. By coupling with sensitive detectors, many ambitious analytical tasks can be fulfilled. Liquid chromatography is the most widely used technique. Different combinations of stationary phases and mobile phases could be used in ion exchange chromatography, ion chromatography, ion-pair reverse-phase chromatography and some other techniques. The application of gas chromatography is limited because only volatile compounds of REEs can be separated. Thin-layer and paper chromatography are techniques that cannot be directly coupled with suitable detectors, which limit their applications. For special demands, separations can be performed by capillary electrophoresis, which has very high separation efficiency.

  7. Chromatographic Separation of Vitamin E Enantiomers

    Directory of Open Access Journals (Sweden)

    Ju-Yen Fu

    2017-02-01

    Full Text Available Vitamin E is recognized as an essential vitamin since its discovery in 1922. Most vegetable oils contain a mixture of tocopherols and tocotrienols in the vitamin E composition. Structurally, tocopherols and tocotrienols share a similar chromanol ring and a side chain at the C-2 position. Owing to the three chiral centers in tocopherols, they can appear as eight different stereoisomers. Plant sources of tocopherol are naturally occurring in the form of RRR while synthetic tocopherols are usually in the form of all-racemic mixture. Similarly, with only one chiral center, natural tocotrienols occur as the R-isoform. In this review, we aim to discuss a few chromatographic methods that had been used to separate the stereoisomers of tocopherols and tocotrienols. These methods include high performance liquid chromatography, gas chromatography and combination of both. The review will focus on method development including selection of chiral columns, detection method and choice of elution solvent in the context of separation efficiency, resolution and chiral purity. The applications for separation of enantiomers in vitamin E will also be discussed especially in terms of the distinctive biological potency among the stereoisoforms.

  8. Chromatographic separation of low-temperature tar. Part II

    Energy Technology Data Exchange (ETDEWEB)

    Terres, E; Gebert, F; Huelsemann, H; Petereit, H; Toepsch, H; Ruppert, W

    1955-01-01

    Chromatographic methods and apparatus are discussed. The Folin-Denis reagent (Na tungstate molybdate in H/sub 3/PO/sub 4/) is used for developing paper chromatograms of phenol mixtures. Other reagents are given.

  9. Chromatographic measurement of hydrogen isotopic and permanent gas impurities in tritium

    International Nuclear Information System (INIS)

    Warner, D.K.; Kinard, C.; Bohl, D.C.

    1976-01-01

    This paper describes a gas chromatograph that was designed for dedicated analysis of hydrogen isotopic and permanent gas impurities in tritium and tritium-deuterium mixtures. The instrument that was developed substantially improved the accuracy and precision of hydrogen isotopic analysis in the 20 ppM to one mole percent range as compared with other analytical methods. Several unique design features of the instrument were required due to the radiation and isotopic exchange properties of the tritium in the samples; descriptions of these features are presented along with details of the complete chromatographic system. The experimental procedures used to calibrate the detector and statistically evaluate its performance are given, and the sources of analytical error are cited. The limitations of the present system are also discussed

  10. Ion Chromatographic Analyses of Sea Waters, Brines and Related Samples

    OpenAIRE

    Nataša Gros

    2013-01-01

    This review focuses on the ion chromatographic methods for the analyses of natural waters with high ionic strength. At the beginning a natural diversity in ionic composition of waters is highlighted and terminology clarified. In continuation a brief overview of other review articles of potential interest is given. A review of ion chromatographic methods is organized in four sections. The first section comprises articles focused on the determination of ionic composition of water samples as com...

  11. Analysis and chromatographic purification of eicosanoids multiply labeled by tritium

    International Nuclear Information System (INIS)

    Shevchenko, V.P.; Nagaev, I.Yu.; Myasoedov, N.F.

    1989-01-01

    We show the possibility of analysis and chromatographic purification of eicosanoids triply labeled by tritium. The described methods allow us to isolate chromatographically pure products obtained by selective hydrogenatin, chemical, and enzyme methods, with radiochemical purity at least 95-97%. The following methods are used to analyze the reaction mixtures and to isolate the tritium-labeled eicosanoids: gas-liquid chromatography, high-efficiency liquid chromatography, and thin-layer chromatography on supports impregnated with silver nitrate

  12. Multidimensional High-Resolution Gas Chromatographic Investigations of Hydrocarbon Fuels and Various Turbine Engine Fuel Precursors.

    Science.gov (United States)

    1985-08-01

    time were spurious transits observed during the recording of the chromatographic output data. *Packaged gas purification filters supplied by Alltech ... Alltech ) that were needed for these unusual installations. When the column diameters were small and of comparable size, the assembly attach- ments at...into an MDGC system has definite advantages as separations can be made faster and with greater detectability. However, specific precautions must be

  13. Radio-chromatographic determination of plasmatic adenosine deaminase (A.D.)

    International Nuclear Information System (INIS)

    Chivot, J.J.; Depernet, D.; Caen, J.

    1970-01-01

    We were able, by using a radio-chromatographic method, to measure an adenosine deaminase activity in normal human heparinized platelet-poor plasma, which can degrade 0.016 μM adenosine. This activity suppressed by heating 56 C for 30 minutes is inhibited by high concentrations of urea and is proportional to the amount of plasma, source of enzyme, in the systems. (authors) [fr

  14. Chromatographic separation of fructose from date syrup.

    Science.gov (United States)

    Al Eid, Salah M

    2006-01-01

    The objective of this study is to provide a process for separating fructose from a mixture of sugars containing essentially fructose and glucose, obtained from date palm fruits. The extraction procedure of date syrup from fresh dates gave a yield of 86.5% solids after vacuum drying. A process for separating fructose from an aqueous solution of date syrup involved adding the date syrup solutions (20, 30 and 40% by weight) to a chromatographic column filled with Dowex polystyrene strong cation exchange gel matrix resin Ca2 + and divinylbenzene, a functional group, sulfonic acid, particle size 320 microm, with a flow rate of 0.025 and 0.05 bed volume/min, under 30 and 70 degrees C column temperature. After the date sugar solution batch, a calculated quantity of water was added to the column. Glucose was retained by the resin more weakly than fructose and proceeded faster into the water batch flowing ahead. Three fractions were collected: a glucose-rich fraction, a return fraction, and a fructose-rich fraction. The return fraction is based on when the peaks of fructose and glucose were reached, which could be determined by means of an analyzer (polarimeter) based on the property of glucose and fructose solutions to turn the polarization level of polarized light. A high yield of fructose is obtained at 70 degrees C column temperature with a flow rate of 0.025 bed volume/min and date syrup solution containing 40% sugar concentration. The low recovery by weight obtained using date syrup solutions having a sugar concentration of 20 and 30%, encourages the use of a concentration of 40%. However, with the 40% date syrup supply the average concentrations of glucose and fructose in the return fractions were more than 40%, which can be used for diluting the thick date syrup solution extracted from dates.

  15. QSAR models for prediction of chromatographic behavior of homologous Fab variants.

    Science.gov (United States)

    Robinson, Julie R; Karkov, Hanne S; Woo, James A; Krogh, Berit O; Cramer, Steven M

    2017-06-01

    While quantitative structure activity relationship (QSAR) models have been employed successfully for the prediction of small model protein chromatographic behavior, there have been few reports to date on the use of this methodology for larger, more complex proteins. Recently our group generated focused libraries of antibody Fab fragment variants with different combinations of surface hydrophobicities and electrostatic potentials, and demonstrated that the unique selectivities of multimodal resins can be exploited to separate these Fab variants. In this work, results from linear salt gradient experiments with these Fabs were employed to develop QSAR models for six chromatographic systems, including multimodal (Capto MMC, Nuvia cPrime, and two novel ligand prototypes), hydrophobic interaction chromatography (HIC; Capto Phenyl), and cation exchange (CEX; CM Sepharose FF) resins. The models utilized newly developed "local descriptors" to quantify changes around point mutations in the Fab libraries as well as novel cluster descriptors recently introduced by our group. Subsequent rounds of feature selection and linearized machine learning algorithms were used to generate robust, well-validated models with high training set correlations (R 2  > 0.70) that were well suited for predicting elution salt concentrations in the various systems. The developed models then were used to predict the retention of a deamidated Fab and isotype variants, with varying success. The results represent the first successful utilization of QSAR for the prediction of chromatographic behavior of complex proteins such as Fab fragments in multimodal chromatographic systems. The framework presented here can be employed to facilitate process development for the purification of biological products from product-related impurities by in silico screening of resin alternatives. Biotechnol. Bioeng. 2017;114: 1231-1240. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

  16. Chromatographic, Spectroscopic and Mass Spectrometric Approaches for Exploring the Habitability of Mars in 2012 and Beyond with the Curiosity Rover

    Science.gov (United States)

    Mahaffy, Paul

    2012-01-01

    The Sample Analysis at Mars (SAM) suite of instruments on the Curiosity Rover of Mars Science Laboratory Mission is designed to provide chemical and isotopic analysis of organic and inorganic volatiles for both atmospheric and solid samples. The goals of the science investigation enabled by the gas chromatograph mass spectrometer and tunable laser spectrometer instruments of SAM are to work together with the other MSL investigations is to quantitatively assess habitability through a series of chemical and geological measurements. We describe the multi-column gas chromatograph system employed on SAM and the approach to extraction and analysis of organic compounds that might be preserved in ancient martian rocks.

  17. Improved stochastic resonance algorithm for enhancement of signal-to-noise ratio of high-performance liquid chromatographic signal

    International Nuclear Information System (INIS)

    Xie Shaofei; Xiang Bingren; Deng Haishan; Xiang Suyun; Lu Jun

    2007-01-01

    Based on the theory of stochastic resonance, an improved stochastic resonance algorithm with a new criterion for optimizing system parameters to enhance signal-to-noise ratio (SNR) of HPLC/UV chromatographic signal for trace analysis was presented in this study. Compared with the conventional criterion in stochastic resonance, the proposed one can ensure satisfactory SNR as well as good peak shape of chromatographic peak in output signal. Application of the criterion to experimental weak signals of HPLC/UV was investigated and the results showed an excellent quantitative relationship between different concentrations and responses

  18. Automation of an ion chromatograph for precipitation analysis with computerized data reduction

    Science.gov (United States)

    Hedley, Arthur G.; Fishman, Marvin J.

    1982-01-01

    Interconnection of an ion chromatograph, an autosampler, and a computing integrator to form an analytical system for simultaneous determination of fluoride, chloride, orthophosphate, bromide, nitrate, and sulfate in precipitation samples is described. Computer programs provided with the integrator are modified to implement ionchromatographic data reduction and data storage. The liquid-flow scheme for the ion chromatograph is changed by addition of a second suppressor column for greater analytical capacity. An additional vave enables selection of either suppressor column for analysis, as the other column is regenerated and stabilized with concentrated eluent.Minimum limits of detection and quantitation for each anion are calculated; these limits are a function of suppressor exhaustion. Precision for replicate analyses of six precipitation samples for fluoride, chloride, orthophosphate, nitrate, and sulfate ranged from 0.003 to 0.027 milligrams per liter. To determine accuracy of results, the same samples were spiked with known concentrations of the above mentioned anions. Average recovery was 108 percent.

  19. Radio Column Chromatographic Assay of H3-Labelled Substances

    International Nuclear Information System (INIS)

    Scharpenseel, H.W.; Menke, K.H.

    1962-01-01

    Combined radio-chromatographic investigations of H 3 -labelled substances are an integral part of the majority of biochemical experiments with H 3 -labelled compounds. H 3 -radio paper chromatography yields, in a scanner with a windowless flow counter, a counting efficiency of 0,5 -1,5%, depending largely on the thickness of the paper and the self-absorption of the labelled compound. The radio gas chromatography of tritiated compounds presents no major problem. Successful use is being made of a combination of a gas chromatograph with a flow ionization chamber and vibrating reed electrometer, a system originated by K. E. Wilzbach and P. Riessz, and improved by H. Dutton, L. Mason and L. Blair. Through the use of ''Teflon'' and silicone-rubber for the insulating parts of the flow ion chamber, it can be operated at close to 300 o C. Radio column chromatography with tritium holds little promise, when the column effluent is spread out as a shallow layer and slowly passes under a windowless flow counter or a scintillation counter, as was successfully tried with C 14 . Liquid scintillation spectrometry is likely to be the chosen method. Essentially, there are two different approaches feasible. These have been compared: 1. The column effluent is passed through a coil of plastic scintillator tubing, which is wound around a ''Plexiglas'' cylinder and placed in a bath of silicone oil in a light pipe with TiO 2 -reflector. Similarly, the HP-containing effluent can be directed through a test vial, filled - very much as in Steinberg's method - with plastic scintillator beads. These two approaches, that operate highly satisfactorily in the case of C 14 , offer low counting efficiencies of less than 1% for H 3 due to the unfavourable surface to volume ratio. 2. The column effluent is combined 1:30 with a mixture of 3:2 toluene/ethanol by the action of a magnet-vibrator before being assayed while passing through a K 40 -free glass - coiled between the analyser- and monitor

  20. Gas chromatographic analysis of volatiles in fluid and gas inclusions

    Science.gov (United States)

    Andrawes, F.; Holzer, G.; Roedder, E.; Gibson, E.K.; Oro, John

    1984-01-01

    Most geological samples and some synthetic materials contain fluid inclusions. These inclusions preserve for us tiny samples of the liquid and/or the gas phase that was present during formation, although in some cases they may have undergone significant changes from the original material. Studies of the current composition of the inclusions provide data on both the original composition and the change since trapping.These inclusions are seldom larger than 1 millimeter in diameter. The composition varies from a single major compound (e.g., water) in a single phase to a very complex mixture in one or more phases. The concentration of some of the compounds present may be at trace levels.We present here some analyses of inclusions in a variety of geological samples, including diamonds. We used a sample crusher and a gas chromatography—mass spectrometry (GC—MS) system to analyze for organic and inorganic volatiles present as major to trace constituents in inclusions. The crusher is a hardened stainless-steel piston cylinder apparatus with tungsten carbide crusing surfaces, and is operated in a pure helium atmosphere at a controlled temperature.Samples ranging from 1 mg to 1 g were crushed and the released volatiles were analyzed using multi-chromatographic columns and detectors, including the sensitive helium ionization detector. Identification of the GC peaks was carried out by GC—MS. This combination of procedures has been shown to provide geochemically useful information on the process involved in the history of the samples analyzed.

  1. High performance liquid chromatographic determination of glucosamine in rat plasma.

    Science.gov (United States)

    Aghazadeh-Habashi, Ali; Sattari, Saeed; Pasutto, Franco; Jamali, Fakhreddin

    2002-01-01

    A high performance liquid chromatographic method was developed for the determination of glucosamine (GlcN) in rat plasma. Internal standard, galactosamine, was added to 100 micro L of plasma containing GlcN followed by precipitation of plasma proteins with acetonitrile. Evaporation of the decanted supernatant solution was accelerated by the addition of methanol. GlcN was derivatized by addition of a solution containing 1-naphthyl isothiocyanate. Sample cleanup included passage through an anion exchange cartridge. Analysis was accomplished by injection of 0.1 mL of the sample solution into an isocratic HPLC system consisting of a C18 column, a mobile phase of acetonitrile: water: acetic acid: triethylamine (4.5: 95.5:0.1:0.05), a flow rate of 0.9 mL/min, and a UV detector set at 254 nm. Galactosamine and GlcN appeared 26 and 29 min post-injection, respectively. The assay was linear over the range of 1.25-400 micro g/mL (CV<10%) with a detection limit of 0.63 microg/mL and a limit of quantification of 1.25 microg/mL. The method was applied to the determination of GlcN in rat plasma after oral administration of 350 mg/kg of GlcN hydrochloride. The present assay is specific, sensitive, precise, and accurate and is suitable for pharmacokinetic studies.

  2. The counterintuitive role of extra-column volume in the determination of column efficiency and scaling of chromatographic processes

    NARCIS (Netherlands)

    Schultze-Jena, A.; Boon, M.A.; Bussmann, P.J.Th.; Janssen, A.E.M.; Padt, van der A.

    2017-01-01

    In industrial liquid separation processes chromatography often has a key function in the optimization of yield and purity. For the design of an industrial system, chromatographic processes are generally simulated using mathematical models, tested and optimized at laboratory level, and then scaled

  3. The counterintuitive role of extra-column volume in the determination of column efficiency and scaling of chromatographic processes

    NARCIS (Netherlands)

    Schultze-Jena, A.; Boon, M.A.; Bussmann, P.J.T.; Janssen, A.E.M.; Padt, A. van der

    2017-01-01

    In industrial liquid separation processes chromatography often has a key function in the optimization of yield and purity. For the design of an industrial system, chromatographic processes are generally simulated using mathematical models, tested and optimized at laboratory level, and then scaled up

  4. Probabilistic peak detection for first-order chromatographic data.

    Science.gov (United States)

    Lopatka, M; Vivó-Truyols, G; Sjerps, M J

    2014-03-19

    We present a novel algorithm for probabilistic peak detection in first-order chromatographic data. Unlike conventional methods that deliver a binary answer pertaining to the expected presence or absence of a chromatographic peak, our method calculates the probability of a point being affected by such a peak. The algorithm makes use of chromatographic information (i.e. the expected width of a single peak and the standard deviation of baseline noise). As prior information of the existence of a peak in a chromatographic run, we make use of the statistical overlap theory. We formulate an exhaustive set of mutually exclusive hypotheses concerning presence or absence of different peak configurations. These models are evaluated by fitting a segment of chromatographic data by least-squares. The evaluation of these competing hypotheses can be performed as a Bayesian inferential task. We outline the potential advantages of adopting this approach for peak detection and provide several examples of both improved performance and increased flexibility afforded by our approach. Copyright © 2014 Elsevier B.V. All rights reserved.

  5. Assessment of the chromatographic lipophilicity of eight cephalosporins on different stationary phases.

    Science.gov (United States)

    Dąbrowska, Monika; Starek, Małgorzata; Komsta, Łukasz; Szafrański, Przemysław; Stasiewicz-Urban, Anna; Opoka, Włodzimierz

    2017-04-01

    The retention behaviors were investigated for a series of eight cephalosporins in thin-layer chromatography (TLC) using stationary phases of RP-2, RP-8, RP-18, NH 2 , DIOL, and CN chemically bonded silica gel. Additionally, various binary mobile phases (water/methanol and water/acetone) were used in different volume proportions. The retention behavior of the analyzed molecules was defined by R M0 constant. In addition, reversed phase high performance liquid chromatography (RP-HPLC) was performed in lipophilicity studies by using immobilized artificial membrane (IAM) stationary phase. Obtained chromatographic data (R M0 and logk' IAM ) were correlated with the lipophilicity, expressed as values of the log calculated (logP calc ) and experimental (logP exp(shake-flask) ) partition coefficient. Principal component analysis (PCA) was applied in order to obtain an overview of similarity or dissimilarity among the analyzed compounds. Hierarchical cluster analysis (HCA) was performed to compare the separation characteristics of the applied stationary phases. This study was undertaken to identify the best chromatographic system and chromatographic data processing method to enable the prediction of logP values. A comprehensive chromatographic investigation into the retention of the analyzed cephalosporins revealed a similar behavior on RP-18, RP-8 and CN stationary phases. The weak correlations obtained between experimental and certain computed lipophilicity indices revealed that R M0 and PC1/RM are relevant lipophilicity parameters and the RP-8, CN and RP-18 plates are appropriate stationary phases for lipophilicity investigation, whereas computational approaches still cannot fully replace experimentation. Copyright © 2017 Elsevier B.V. All rights reserved.

  6. Chromatographic methods for the simultaneous determination of binary mixture of Saxagliptin HCl and Metformin HCl

    Directory of Open Access Journals (Sweden)

    Hanan A. Merey

    2017-12-01

    Full Text Available Two chromatographic methods were suggested for the simultaneous determination of a binary mixture containing Saxagliptin HCl (SAG and Metformin HCl (MET. First method was RP-HPLC method. Chromatographic separation was done on Kinetex™ column–C18 (4.6 × 150 mm, 2.6 µm using mobile phase consisted of acetonitrile:phosphate buffer pH = 4.5 ± 0.1 adjusted with orthophosphoric acid (13:87, v/v. Isocratic elution at a flow rate 1.5 mL/min and UV detection at 220.0 nm was performed. Second method was spectro-densitometric method. Chromatographic separation was done on precoated silica gel aluminium plates 60 F254 as a stationary phase and developing system consisting of chloroform:methanol:formic acid (80:20:0.3, by volume. The density of the separated bands was measured by UV detector at 210.0 nm. The proposed methods were validated as per the ICH guidelines parameters like Linearity, precision, accuracy, selectivity, limit of detection and limit of quantitation. Statistical comparison was done between the obtained results and those obtained by the reported methods, showing no significant difference with respect to accuracy and precision. Keywords: Saxagliptin HCl, Metformin HCl, RP-HPLC, TLC

  7. Development and validation of micellar liquid chromatographic methods for the determination of antibiotics in different matrixes.

    Science.gov (United States)

    Rambla-Alegre, Maria; Esteve-Romero, Josep; Carda-Broch, Samuel

    2011-01-01

    Antibiotics are the most important bioactive and chemotherapeutic compounds to be produced by microbiological synthesis, and they have proved their worth in a variety of fields, such as medicinal chemistry, agriculture, and the food industry. Interest in antibiotics has grown in parallel with an increasingly high degree of productivity in the field of analytical applications. Therefore, it is necessary to develop chromatographic procedures capable of determining various drugs simultaneously in the shortest possible time. Micellar liquid chromatography (MLC) is an RP-HPLC technique that offers advantages over conventional HPLC as far as sample preparation, selectivity, and versatility are concerned. Its main advantage is that samples can be injected directly into the chromatographic system with no previous preparation step. This paper mainly focuses on the results of the authors' own recent research and reports the chromatographic conditions for determination of various antibiotics (penicillins, quinolones, and sulfonamides) in different matrixes (pharmaceuticals, biological fluids, and food). The work of other authors on MLC-based antibiotic determination has been included.

  8. 15 signals evidence the energy transition is underway

    International Nuclear Information System (INIS)

    2016-09-01

    World leaders approved a universal climate agreement in Paris last year, drawing a line in the sand for the transformation of the world's energy system into a clean and sustainable form. Signs that a global energy transition is happening are everywhere to be seen: in the growth of renewables, the rise of city-level climate actions, the stagnation of CO 2 emissions and in companies who are committing to science based targets as the foundation of their climate actions, to name just a few. We felt it important to highlight 15 key signs or 'signals' of the energy transition, to help raise awareness that the transition to a new, sustainable, equitable, just and fair global energy system has irrevocably begun. What is needed now is to accelerate the transition and scale it up to have a chance of keeping global temperature rise to well below 2 deg. C, trying for 1.5 deg. C, as countries committed to in the Paris Agreement just eight months ago. Global meetings such as the G20 meeting taking place in China on 4-5 September; and the next UN climate talks starting in Marrakech on 7 November gives opportunity to all stakeholders to say how and what they can contribute to accelerating the transition. Ending fossil fuel subsidies, supporting the deployment of renewables and energy efficiency, increasing green finance and putting a just price on carbon are just some of the actions that can be taken right now to put pace into the transition. As 2016 heads into the record books as likely the hottest year ever recorded in history, it is a reminder that we have precious little time left to act to keep global temperatures well below 2 deg. C. We have the Paris Agreement to guide our way. Now we need to implement it. Now is the time for action

  9. Second-order Data by Flow Injection Analysis with Spectrophotometric Diode-array Detection and Incorporated Gel-filtration Chromatographic Column

    DEFF Research Database (Denmark)

    Bechmann, Iben Ellegaard

    1997-01-01

    A flow injection analysis (FIA) system furnished with a gel-filtration chromatographic column and with photodiode-array detection was used for the generation of second-order data. The system presented is a model system in which the analytes are blue dextran, potassium hexacyanoferrate(III) and he......A flow injection analysis (FIA) system furnished with a gel-filtration chromatographic column and with photodiode-array detection was used for the generation of second-order data. The system presented is a model system in which the analytes are blue dextran, potassium hexacyanoferrate...

  10. Zero-determinant strategy: An underway revolution in game theory

    International Nuclear Information System (INIS)

    Hao Dong; Rong Zhi-Hai; Zhou Tao

    2014-01-01

    Repeated games describe situations where players interact with each other in a dynamic pattern and make decisions according to outcomes of previous stage games. Very recently, Press and Dyson have revealed a new class of zero-determinant (ZD) strategies for the repeated games, which can enforce a fixed linear relationship between expected payoffs of two players, indicating that a smart player can control her unwitting co-player's payoff in a unilateral way [Proc. Acad. Natl. Sci. USA 109, 10409 (2012)]. The theory of ZD strategies provides a novel viewpoint to depict interactions among players, and fundamentally changes the research paradigm of game theory. In this brief survey, we first introduce the mathematical framework of ZD strategies, and review the properties and constrains of two specifications of ZD strategies, called pinning strategies and extortion strategies. Then we review some representative research progresses, including robustness analysis, cooperative ZD strategy analysis, and evolutionary stability analysis. Finally, we discuss some significant extensions to ZD strategies, including the multi-player ZD strategies, and ZD strategies under noise. Challenges in related research fields are also listed. (topical review - statistical physics and complex systems)

  11. On-line gas chromatographic analysis of airborne particles

    Science.gov (United States)

    Hering, Susanne V [Berkeley, CA; Goldstein, Allen H [Orinda, CA

    2012-01-03

    A method and apparatus for the in-situ, chemical analysis of an aerosol. The method may include the steps of: collecting an aerosol; thermally desorbing the aerosol into a carrier gas to provide desorbed aerosol material; transporting the desorbed aerosol material onto the head of a gas chromatography column; analyzing the aerosol material using a gas chromatograph, and quantizing the aerosol material as it evolves from the gas chromatography column. The apparatus includes a collection and thermal desorption cell, a gas chromatograph including a gas chromatography column, heated transport lines coupling the cell and the column; and a quantization detector for aerosol material evolving from the gas chromatography column.

  12. Chromatographic method of measurement of helium concentration in underground waters for dating in hydrological questions

    International Nuclear Information System (INIS)

    Najman, J.

    2008-04-01

    Research methods which use natural environmental indicators are widely applied in hydrology. Different concentrations of indicators and their isotopic components in ground waters allow to determine the genesis of waters and are valuable source of information about the water flow dynamics. One of the significant indicator is helium. The concentration of 4 He (helium) in ground water is a fine indicator in water dating in a range from a hundreds to millions of years (Aeschbach-Hertig i in., 1999; Andrews i in., 1989; Castro i in., 2000; Zuber i in., 2007). 4 He is also used for dating young waters of age about 10 years (Solomon i in., 1996). Thesis consist the description of elaborated in IFJ PAN in Krakow chromatographic measurement method of helium concentration in ground waters in aim of dating. Chapter 1 contain short introduction about ground water dating and chapter 2 description of helium property and chosen applications of helium for example in technology and earthquake predictions. Helium sources in ground waters are described in chapter 3. Helium concentration in water after infiltration (originated from atmosphere) to the ground water system depends mainly on the helium concentration coming from the equilibration with the atmosphere increased by additional concentration from '' excess air ''. With the increasing resistance time of ground water during the flow, radiogenic, non-atmospheric component of helium dissolves also in water. In chapter 4 two measurement methods of helium concentration in ground waters were introduced: mass spectrometric and gas chromatographic method. Detailed description of elaborated chromatographic measurement method of helium concentration in ground water contain chapter 5. To verify developed method the concentration of helium in ground waters from the regions of Krakow and Busko Zdroj were measured. For this waters the concentrations of helium are known from the earlier mass spectrometric measurements. The results of

  13. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Bell M. Shimada in the Coastal Waters of SE Alaska and North Pacific Ocean from 2016-03-16 to 2016-03-20 (NCEI Accession 0151240)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0151240 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  14. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Oscar Dyson in the Coastal Waters of Southeast Alaska and British Columbia, Gulf of Alaska and North Pacific Ocean from 2016-10-13 to 2016-10-19 (NCEI Accession 0165091)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0165091 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  15. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Oscar Dyson in the Coastal Waters of SE Alaska, Gulf of Alaska and North Pacific Ocean from 2013-04-04 to 2013-04-15 (NODC Accession 0124185)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0124185 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  16. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Oscar Dyson in the Coastal Waters of SE Alaska, Gulf of Alaska and North Pacific Ocean from 2016-01-30 to 2016-02-09 (NCEI Accession 0150729)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0150729 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  17. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Oscar Dyson in the Coastal Waters of SE Alaska, Gulf of Alaska and North Pacific Ocean from 2016-02-03 to 2016-02-09 (NCEI Accession 0150730)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0150730 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  18. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Bell M. Shimada in the Coastal Waters of Southeast Alaska and British Columbia and North Pacific Ocean from 2015-09-16 to 2015-10-13 (NCEI Accession 0135733)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0135733 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  19. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Reuben Lasker in the Coastal Waters of SE Alaska, Gulf of Alaska and others from 2015-07-09 to 2015-10-22 (NCEI Accession 0132081)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0132081 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  20. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Bell M. Shimada in the Coastal Waters of Southeast Alaska and British Columbia and North Pacific Ocean from 2016-06-30 to 2016-08-14 (NCEI Accession 0162237)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0162237 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  1. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Bell M. Shimada in the Coastal Waters of SE Alaska and North Pacific Ocean from 2014-06-24 to 2014-09-14 (NCEI Accession 0121964)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0121964 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  2. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Bell M. Shimada in the Coastal Waters of SE Alaska and North Pacific Ocean from 2015-02-11 to 2015-03-03 (NODC Accession 0126536)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0126536 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  3. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Oscar Dyson in the Coastal Waters of Southeast Alaska and British Columbia, Gulf of Alaska and North Pacific Ocean from 2017-01-26 to 2017-02-01 (NCEI Accession 0165022)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0165022 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  4. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Oscar Dyson in the Coastal Waters of SE Alaska, Gulf of Alaska and North Pacific Ocean from 2013-09-24 to 2013-11-03 (NODC Accession 0124206)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0124206 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  5. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Oscar Dyson in the Coastal Waters of SE Alaska, Gulf of Alaska and North Pacific Ocean from 2015-01-24 to 2015-01-30 (NODC Accession 0126876)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0126876 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  6. Underway meteorological, navigational, physical and time series data collected aboard NOAA Ship Ronald H. Brown in the Coastal Waters of SE Alaska, Gulf of Alaska and North Pacific Ocean from 2015-07-14 to 2015-08-03 (NCEI Accession 0130369)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0130369 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...

  7. Underway meteorological, navigational, physical and time series data collected aboard NOAA Ship Ronald H. Brown in the Coastal Waters of Southeast Alaska and British Columbia, Columbia River estuary - Washington/Oregon and North Pacific Ocean from 2016-05-05 to 2016-06-07 (NCEI Accession 0155887)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0155887 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...

  8. Underway meteorological, navigational, optical, physical and time series data collected aboard NOAA Ship Oscar Dyson in the Coastal Waters of SE Alaska, Gulf of Alaska and North Pacific Ocean from 2013-01-28 to 2013-02-03 (NODC Accession 0124297)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0124297 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...

  9. Partial pressure (or fugacity) of carbon dioxide, temperature, salinity and other variables collected from the coastal surface underway observations using carbon dioxide gas analyzer, shower head equilibrator and other instruments from NOAA Ship Gordon Gunter in the North Atlantic Ocean, US North-East coast during 2017 (NCEI Accession 0163566)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — In March, 2008, the Ocean Carbon Cycle (OCC) group at NOAA's Atlantic Oceanographic and Meteorological Laboratory (AOML) installed an underway system to measure...

  10. Underway meteorological, navigational, optical, physical, profile and time series data collected aboard NOAA Ship Bell M. Shimada in the Coastal Waters of SE Alaska and North Pacific Ocean from 2015-06-20 to 2015-09-10 (NCEI Accession 0131258)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0131258 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...

  11. Chromatographic air analyser microsystem for the selective and sensitive detection of atmospheric pollutants

    International Nuclear Information System (INIS)

    Sanchez, Jean-Baptiste; Lahlou, Houda; Mohsen, Yehya; Berger, Franck; Vilanova, Xavier; Correig, Xavier

    2011-01-01

    The development of industry and automotive trafic produces Volatile Organic Compounds (VOCs) whose toxicity can affect seriously human health and environment. The level of those contaminants in air must be as low as possible. In this context, there is a need for in situ systems that could monitor selectively the concentration of these compounds. The aim of this study is to demonstrate the efficiency of a system build with a pre-concentrator, a chromatographic micro-column and a tin oxide-based gas sensor for the selective and sensitive detection of atmospheric pollutants. In particular, this study is focused on the selective detection of benzene and 1,3 butadiene.

  12. Chromatographic quality control procedures for /sup 99m/Tc-diagnostic agents

    International Nuclear Information System (INIS)

    Marinelli, M.; Pozzato, R.; Garuti, P.; Zucchini, G.L.

    1986-01-01

    The purpose of this work was to experiment simple and rapid chromatographic systems, based on paper and thin-layer techniques, to test the radiochemical purity of some common /sup 99m/Tc diagnostic agents, and select those systems able to prevent the anomalies due to oxidation and artifact production. The agents were examined under conditions which usually bring about the above mentioned anomalies, then the results were compared with those obtained under controlled conditions. Quali- and quantitative detection of the activity present on the chromatograms was carried out using the equipment available in nuclear medicine departments

  13. Mars Solar Balloon Landed Gas Chromatograph Mass Spectrometer

    Science.gov (United States)

    Mahaffy, P.; Harpold, D.; Niemann, H.; Atreya, S.; Gorevan, S.; Israel, G.; Bertaux, J. L.; Jones, J.; Owen, T.; Raulin, F.

    1999-01-01

    A Mars surface lander Gas Chromatograph Mass Spectrometer (GCMS) is described to measure the chemical composition of abundant and trace volatile species and isotope ratios for noble gases and other elements. These measurements are relevant to the study of atmospheric evolution and past climatic conditions. A Micromission plan is under study where a surface package including a miniaturized GCMS would be delivered to the surface by a solar heated hot air balloon based system. The balloon system would be deployed about 8 km above the surface of Mars, wherein it would rapidly fill with Martian atmosphere and be heated quickly by the sun. The combined buoyancy and parachuting effects of the solar balloon result in a surface package impact of about 5 m/sec. After delivery of the package to the surface, the balloon would ascend to about 4 km altitude, with imaging and magnetometry data being taken for the remainder of the daylight hours as the balloon is blown with the Martian winds. Total atmospheric entry mass of this mission is estimated to be approximately 50 kg, and it can fit as an Ariane 5 piggyback payload. The GCMS would obtain samples directly from the atmosphere at the surface and also from gases evolved from solid phase material collected from well below the surface with a Sample Acquisition and Transport Mechanism (SATM). The experiment envisioned in the Mars Micromission described would obtain samples from a much greater depth of up to one meter below the surface, and would search for organic molecules trapped in ancient stratified layers well below the oxidized surface. Insitu instruments on upcoming NASA missions working in concert with remote sensing measurement techniques have the potential to provide a more detailed investigation of mineralogy and the extent of simple volatiles such as CO2 and H2O in surface and subsurface solid phase materials. Within the context of subsequent mission opportunities such as those provided by the Ariane 5 piggyback

  14. Sensitive high performance liquid chromatographic method for the ...

    African Journals Online (AJOL)

    A new simple, sensitive, cost-effective and reproducible high performance liquid chromatographic (HPLC) method for the determination of proguanil (PG) and its metabolites, cycloguanil (CG) and 4-chlorophenylbiguanide (4-CPB) in urine and plasma is described. The extraction procedure is a simple three-step process ...

  15. Bioanalytical method transfer considerations of chromatographic-based assays.

    Science.gov (United States)

    Williard, Clark V

    2016-07-01

    Bioanalysis is an important part of the modern drug development process. The business practice of outsourcing and transferring bioanalytical methods from laboratory to laboratory has increasingly become a crucial strategy for successful and efficient delivery of therapies to the market. This chapter discusses important considerations when transferring various types of chromatographic-based assays in today's pharmaceutical research and development environment.

  16. Development and Validation of a Liquid Chromatographic Method ...

    African Journals Online (AJOL)

    A liquid chromatographic method for the simultaneous determination of six human immunodeficiency virus (HIV) protease inhibitors, indinavir, saquinavir, ritonavir, amprenavir, nelfinavir and lopinavir, was developed and validated. Optimal separation was achieved on a PLRP-S 100 Å, 250 x 4.6 mm I.D. column maintained ...

  17. Gas chromatographic column for the Viking 1975 molecular analysis experiment

    Science.gov (United States)

    Novotny, M.; Hayes, J. M.; Bruner, F.; Simmonds, P. G.

    1975-01-01

    A gas chromatographic column has been developed for use in the remote analysis of the Martian surface. The column, which utilizes a liquid-modified organic adsorbent (Tenax) as the stationary phase, provides efficient transmission and resolution of nanogram quantities of organic materials in the presence of millionfold excesses of water and carbon dioxide.

  18. Optimizing Chromatographic Separation: An Experiment Using an HPLC Simulator

    Science.gov (United States)

    Shalliker, R. A.; Kayillo, S.; Dennis, G. R.

    2008-01-01

    Optimization of a chromatographic separation within the time constraints of a laboratory session is practically impossible. However, by employing a HPLC simulator, experiments can be designed that allow students to develop an appreciation of the complexities involved in optimization procedures. In the present exercise, a HPLC simulator from "JCE…

  19. Radioimmunoassay of methaqualone in human urine compared with chromatographic methods

    International Nuclear Information System (INIS)

    Mule, S.J.; Kogan, M.; Jukofsky, D.

    1978-01-01

    The 125 I-radioimmunoassay for methaqualone in human urine was evaluated by a comparison with newly modified gas-liquid chromatographic and thin-layer chromatographic methods. The statistically significant sensitivity value for the radioimmunoassay was at 2 μg of methaqualone per liter of urine. The coefficient of variation was 2.88 -+ 0.16% intraassay. There was cross-reactivity only with metabolites of methaqualone, 4'-hydroxymethaqualone being twice as sensitively measured as methaqualone. There was complete agreement between results by radioimmunoassay and by gas-liquid chromatography in 96.7% of the samples analyzed. Only 1.2% of the radioimmunoassay values were false positives, and 2.1% false negatives (phi = 0.8917, P < 0.001). Comparisons between the thin-layer chromatographic data and the gas--liquid chromatographic or radioimmunoassay data showed less agreement because of the 50- to 200-fold higher sensitivity of the latter techniques. Gas--liquid chromatography therefore appears to represent the best reference method for the evaluation of the radioimmunoassay, which appears to be a very sensitive and reliable technique for detecting methaqualone and its metabolites in human urine

  20. Coupling spectroscopic and chromatographic techniques for evaluation of the depositional history of hydrocarbons in a subtropical estuary

    International Nuclear Information System (INIS)

    Martins, César C.; Doumer, Marta E.; Gallice, Wellington C.; Dauner, Ana Lúcia L.; Cabral, Ana Caroline; Cardoso, Fernanda D.

    2015-01-01

    Spectroscopic and chromatographic techniques can be used together to evaluate hydrocarbon inputs to coastal environments such as the Paranaguá estuarine system (PES), located in the SW Atlantic, Brazil. Historical inputs of aliphatic hydrocarbons (AHs) and polycyclic aromatic hydrocarbons (PAHs) were analyzed using two sediment cores from the PES. The AHs were related to the presence of biogenic organic matter and degraded oil residues. The PAHs were associated with mixed sources. The highest hydrocarbon concentrations were related to oil spills, while relatively low levels could be attributed to the decrease in oil usage during the global oil crisis. The results of electron paramagnetic resonance were in agreement with the absolute AHs and PAHs concentrations measured by chromatographic techniques, while near-infrared spectroscopy results were consistent with unresolved complex mixture (UCM)/total n-alkanes ratios. These findings suggest that the use of a combination of techniques can increase the accuracy of assessment of contamination in sediments. - Highlights: • Historical inputs of hydrocarbons in a subtropical estuary were evaluated. • Spectroscopic and chromatographic methods were used in combination. • High hydrocarbon concentrations were related to anthropogenic activities. • Low hydrocarbon levels could be explained by the 1970s global oil crisis. - Spectroscopic and chromatographic techniques could be used together to evaluate hydrocarbon inputs to coastal environments

  1. Implementation of a fully automated process purge-and-trap gas chromatograph at an environmental remediation site

    International Nuclear Information System (INIS)

    Blair, D.S.; Morrison, D.J.

    1997-01-01

    The AQUASCAN, a commercially available, fully automated purge-and-trap gas chromatograph from Sentex Systems Inc., was implemented and evaluated as an in-field, automated monitoring system of contaminated groundwater at an active DOE remediation site in Pinellas, FL. Though the AQUASCAN is designed as a stand alone process analytical unit, implementation at this site required additional hardware. The hardware included a sample dilution system and a method for delivering standard solution to the gas chromatograph for automated calibration. As a result of the evaluation the system was determined to be a reliable and accurate instrument. The AQUASCAN reported concentration values for methylene chloride, trichloroethylene, and toluene in the Pinellas ground water were within 20% of reference laboratory values

  2. Aspect-dependent radiated noise analysis of an underway autonomous underwater vehicle.

    Science.gov (United States)

    Gebbie, John; Siderius, Martin; Allen, John S

    2012-11-01

    This paper presents an analysis of the acoustic emissions emitted by an underway REMUS-100 autonomous underwater vehicle (AUV) that were obtained near Honolulu Harbor, HI using a fixed, bottom-mounted horizontal line array (HLA). Spectral analysis, beamforming, and cross-correlation facilitate identification of independent sources of noise originating from the AUV. Fusion of navigational records from the AUV with acoustic data from the HLA allows for an aspect-dependent presentation of calculated source levels of the strongest propulsion tone.

  3. High-pressure liquid chromatographic analysis of pramoxine hydrochloride in high lipoid aerosol foam dosage form.

    Science.gov (United States)

    Weinberger, R; Mann, B; Posluszny, J

    1980-04-01

    A rapid and quantitative method for the determination of pramoxine hydrochloride by high-pressure liquid chromatography is presented. The drug is extracted as the salt from a preparation with a high lipoid composition by partitioning it to the aqueous phase of an ether-methanol-water-acetic acid system. The extract is chromatographed on an octadecylsilane bonded packing with a methanol-water-acetic acid-methanesulfonic acid mobile phase. The time required for each separation is approximately 6 min. Analytical recoveries of 100.4 +/- 1.5% were obtained.

  4. Multiple-channel ultra-violet absorbance detector for two-dimensional chromatographic separations.

    Science.gov (United States)

    Lynch, Kyle B; Yang, Yu; Ren, Jiangtao; Liu, Shaorong

    2018-05-01

    In recent years, much research has gone into developing online comprehensive two-dimensional liquid chromatographic systems allowing for high peak capacities in comparable separation times to that of one-dimensional liquid chromatographic systems. However, the speed requirements in the second dimension (2nd-D) still remain one challenge for complex biological samples due to the current configuration of two column/two detector systems. Utilization of multiple 2nd-D columns can mitigate this challenge. To adapt this approach, we need a multiple channel detector. Here we develop a versatile multichannel ultraviolet (UV) light absorbance detector that is capable of simultaneously monitoring separations in 12 columns. The detector consists of a deuterium lighthouse, a flow cell assembly (a 13-channel flow cell fitted with a 13-photodiode-detection system), and a data acquisition and monitoring terminal. Through the use of a custom high optical quality furcated fiber to improve light transmission, precise machining of a flow cell to reduce background stray light through precision alignment, and sensitive electronic circuitry to reduce electronic noise through an active low pass filter, the background noise level is measured in the tens of µAU. We obtain a linear dynamic range of close to three orders of magnitude. Compared to a commercialized multichannel UV light absorbance detector like the Waters 2488 UV/Vis, our device provides an increase in channel detection while residing within the same noise region and linear range. Copyright © 2018 Elsevier B.V. All rights reserved.

  5. Rapid validated liquid chromatographic method coupled with ...

    African Journals Online (AJOL)

    NTB in human plasma, and thus can be applied efficiently for the analysis of clinical samples containing. NTB. Keywords: ... permits the analysis of a lot of clinical samples containing NTB in a ... from Milli-Q plus purification system, Millipore, ... Fifty microliter of CBP working standard solution ... Percentage relative error (%.

  6. An on-line push/pull perfusion-based hollow-fiber liquid-phase microextraction system for high-performance liquid chromatographic determination of alkylphenols in water samples.

    Science.gov (United States)

    Chao, Yu-Ying; Jian, Zhi-Xuan; Tu, Yi-Ming; Wang, Hsaio-Wen; Huang, Yeou-Lih

    2013-06-07

    In this study, we employed a novel on-line method, push/pull perfusion hollow-fiber liquid-phase microextraction (PPP-HF-LPME), to extract 4-tert-butylphenol, 2,4-di-tert-butylphenol, 4-n-nonylphenol, and 4-n-octylphenol from river and tap water samples; we then separated and quantified the extracted analytes through high-performance liquid chromatography (HPLC). Using this approach, we overcame the problem of fluid loss across the porous HF membrane to the donor phase, permitting on-line coupling of HF-LPME to HPLC. In our PPP-HF-LPME system, we used a push/pull syringe pump as the driving source to perfuse the acceptor phase, while employing a heating mantle and an ultrasonic probe to accelerate mass transfer. We optimized the experimental conditions such as the nature of the HF supported intermediary phase and the acceptor phase, the composition of the donor and acceptor phases, the sample temperature, and the sonication conditions. Our proposed method provided relative standard deviations of 3.1-6.2%, coefficients of determination (r(2)) of 0.9989-0.9998, and limits of detection of 0.03-0.2 ng mL(-1) for the analytes under the optimized conditions. When we applied this method to analyses of river and tap water samples, our results confirmed that this microextraction technique allows reliable monitoring of alkylphenols in water samples.

  7. Comparison of core-shell and totally porous ultra high performance liquid chromatographic stationary phases based on their selectivity towards alfuzosin compounds.

    Science.gov (United States)

    Szulfer, Jarosław; Plenis, Alina; Bączek, Tomasz

    2014-06-13

    This paper focuses on the application of a column classification system based on the Katholieke Universiteit Leuven for the characterization of physicochemical properties of core-shell and ultra-high performance liquid chromatographic stationary phases, followed by the verification of the reliability of the obtained column classification in pharmaceutical practice. In the study, 7 stationary phases produced in core-shell technology and 18 ultra-high performance liquid chromatographic columns were chromatographically tested, and ranking lists were built on the FKUL-values calculated against two selected reference columns. In the column performance test, an analysis of alfuzosin in the presence of related substances was carried out using the brands of the stationary phases with the highest ranking positions. Next, a system suitability test as described by the European Pharmacopoeia monograph was performed. Moreover, a study was also performed to achieve a purposeful shortening of the analysis time of the compounds of interest using the selected stationary phases. Finally, it was checked whether methods using core-shell and ultra-high performance liquid chromatographic columns can be an interesting alternative to the high-performance liquid chromatographic method for the analysis of alfuzosin in pharmaceutical practice. Copyright © 2014 Elsevier B.V. All rights reserved.

  8. Micro-column plasma emission liquid chromatograph. [Patent application

    Science.gov (United States)

    Gay, D.D.

    1982-08-12

    In a direct current plasma emission spectrometer for use in combination with a microcolumn liquid chromatograph, an improved plasma source unit is claimed. The plasma source unit includes a quartz capillary tube having an inlet means, outlet off gas means and a pair of spaced electrodes defining a plasma region in the tube. The inlet means is connected to and adapted to receive eluant of the liquid chromatograph along with a stream of plasma-forming gas. There is an opening through the wall of the capillary tube penetrating into the plasma region. A soft glass capillary light pipe is disposed at the opening, is connected to the spectrometer, and is adapted to transmit light passing from the plasma region to the spectrometer. There is also a source of electromotive force connected to the electrodes sufficient to initiate and sustain a plasma in the plasma region of the tube.

  9. Chiral ionic liquids in chromatographic and electrophoretic separations.

    Science.gov (United States)

    Kapnissi-Christodoulou, Constantina P; Stavrou, Ioannis J; Mavroudi, Maria C

    2014-10-10

    This report provides an overview of the application of chiral ionic liquids (CILs) in separation technology, and particularly in capillary electrophoresis and both gas and liquid chromatography. There is a large number of CILs that have been synthesized and designed as chiral agents. However, only a few have successfully been applied in separation technology. Even though this application of CILs is still in its early stages, the scientific interest is increasing dramatically. This article is focused on the use of CILs as chiral selectors, background electrolyte additives, chiral ligands and chiral stationary phases in electrophoretic and chromatographic techniques. Different examples of CILs, which contain either a chiral cation, a chiral anion or both, are presented in this review article, and their major advantages along with their potential applications in chiral electrophoretic and chromatographic recognition are discussed. Copyright © 2014 Elsevier B.V. All rights reserved.

  10. Gas-chromatographic separation of hydrogen isotopic mixtures

    International Nuclear Information System (INIS)

    Preda, Anisoara; Bidica, Nicolae

    2005-01-01

    Full text: Gas chromatographic separation of hydrogen isotopes have been reported in the literature since late of 1950's. Gas chromatography is primarily an analytical method, but because of its properties it may be used in many other fields with excellent results. A simple method is proposed for the gas-chromatographic analysis of complex gas mixtures containing hydrogen isotopes; the method is based on the substantial difference in the thermal conductivity of these isotopes. One of the main disadvantages of the conventional gas chromatography is the long retention times required for the analysis of hydrogen gas mixtures while the column is operated at very low temperature. The method described in this paper was based on using a capillary molecular sieve 5A column operated for this kind of separation at 173 K. The carrier gas was Ne and the detector was TCD. In the paper chromatograms for various carrier flow rates and various hydrogen isotope mixtures are presented. (authors)

  11. Chromatographic determination of Terbinafine in presence of its Photodegradation products

    International Nuclear Information System (INIS)

    Abdel-Moety, E.M.; Kelani, K.O.; Abou Al-Alamein, A.M.

    2003-01-01

    Two different chromatographic techniques have been developed for the determination of terbinafine hydrochloride in presence of its photodegradation products. The first method depends on coupling the TLC- fractionation, on silicia gel 60F254 utilizing chloroform+methanol+25% aq. ammonia ( 12: 0.1:0.1,by volumes), with the direct scanning at 284nm. The second method describes a liquid chromatographic separation of terbinafine and its photolytic degradates on a reversed-phase column[u-Bondapak-TMC 18 (10um,25cmx4.6mm,i.d.)] using a mobile phase containing methanol+water= 80:20 (v/v) with UV-detection at 284 nm. The proposed methods showed significant stability-indication with good linearity, precision and reproducibility. (author)

  12. Paper chromatographic behavior of tetranitronitrosylruthenate and its decomposition products

    International Nuclear Information System (INIS)

    Akatsu, Eiko

    1981-01-01

    The paper chromatographic behavior of tetranitronitrosylruthenate was examined together with its decomposition products. First, Rf values at various eluting conditions were studied with five kinds of papers, since there was a discrepancy among the reported Rf values. Usually Rf of 0.9 was obtained by the recommended procedure of Wain, et al. Second, stability of the tetranitrosylruthenate in water was followed by paper chromatography. No change was found until 200 days passed. Third, the tetranitronitrosylruthenate was warmed in acid, and its decomposition products were studied through the paper chromatography. The paper chromatographic behavior showed that the sample kept in water more than 200 days seemed to contain a dinitro complex, and the sample warmed in acid seemed to be decomposed to a mononitro complex of further. (author)

  13. Studies on Brucella interferon: Chromatographic behaviour and purification

    International Nuclear Information System (INIS)

    Bousquet-Ucla, C.; Wietzerbin, J.; Falcoff, E.

    1980-01-01

    Interferon was induced by infecting mice with Brucella suis. Serum containing interferon activity was analyzed by chromatography on Concanavalin A-Sepharose and Phenyl-Sepharose CL-4B columns. Antiviral activity was completely retained by the lectin column indicating that all the interferon molecules are glycosylated. The chromatographic behaviour of Brucella interferon on Phenyl-Sepharose CL-4B show that, like other interferons, Brucella interferon displays hydrophobic properties. However, the hydrophobicity of the interferon molecule was masked in the crude preparation and was only detectable when purified Brucella interferon was used for chromatography. The antigenic properties of Brucella interferon provided the means for developing an affinity chromatographic method resulting in about 60.000 fold purification. As in the case of viral interferon, treatment of L cells with Brucella interferon induced specific enhanced in vitro phosphorylation of a 67.000 molecular weight protein after incubation of cell extracts with doublestranded RNA and [γ- 32 p]ATP. (auth.)

  14. Chromatographic decontamination of medium-activity waste concentrates

    International Nuclear Information System (INIS)

    Faubel, W.; Menzler, P.M.; Sameh, A.A.

    1988-01-01

    The chromatographic decontamination of a MAW concentrate was carried out in a laboratory plant in 1-l-batches in the following way: In order to purify the nitric MAW concentrate from its solid and organic contamination products, it is passed through a filter and an absorber (SM7) for organic species. Subsequently the purified solution runs on-line through all following columns. First the main activity carrier cesium ( 137 Cs, 134 Cs) is transferred to ammonium molybdate phosphate (AMP-1) by means of a newly developed fluidized bed process. In the further course, 125 Sb is separated on metal oxides (Sb 2 O 5 , MnO 2 ) and the three-valued actinides/lanthanides on an extraction-chromatographic CMPO column. Finally the remaining 106 Ru and 60 Co activities are separated on dimethylglyoximes (DMG) coated on active carbon. (orig./RB) [de

  15. Measurement Uncertainty of Liquid Chromatographic Analyses Visualized by Ishikawa Diagrams

    OpenAIRE

    Meyer, Veronika R.

    2017-01-01

    Ishikawa, or cause-and-effect diagrams, help to visualize the parameters that influence a chromatographic analysis. Therefore, they facilitate the set up of the uncertainty budget of the analysis, which can then be expressed in mathematical form. If the uncertainty is calculated as the Gaussian sum of all uncertainty parameters, it is necessary to quantitate them all, a task that is usually not practical. The other possible approach is to use the intermediate precision as a base for the uncer...

  16. Liquid chromatographic-tandem mass spectrometric assay for ...

    African Journals Online (AJOL)

    Methods: Blood and urine samples were obtained from healthy volunteers who admitted to not being on any medications. The investigated analytes were chromatographically separated on a C18 column (Luna®-PFP 100Å column, 50 mm × 2.0 mm i.d., 3.0 μm) with the aid of a mobile phase containing A; acetonitrile (ACN) ...

  17. The development of a high performance liquid chromatograph with a sensitive on-stream radioactivity monitor for the analysis of 3H- and 14C-labelled gibberellins

    International Nuclear Information System (INIS)

    Reeve, D.R.; Yokota, T.; Nash, L.; Crozier, A.

    1976-01-01

    The development of a high performance liquid chromatograph for the separation of gibberellins is described. The system combines high efficiency, peak capacity, and sample capacity with rapid speed of analysis. In addition, the construction details of a sensitive on-stream radioactivity monitor are outlined. The overall versatility of the chromatograph has been demonstrated by the separation of a range of 3 H- and 14 C-labelled gibberellins and gibberellin precursors. The system also has considerable potential for the analysis of abscisic acid and acidic and neutral indoles. (author)

  18. Sea surface temperature and salinity from the Global Ocean Surface Underway Data (GOSUD) from 1980-01-03 to present

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This collection contains the Global Ocean Surface Underway Data (GOSUD) from 1980-01-03 to present as submitted to NOAA/NCEI. The data includes information about sea...

  19. Reverse-Phase High-Performance Liquid Chromatographic Separation of Methylated and Non-Methylated Nucleic Acid Bases

    OpenAIRE

    Madyastha, Prema; Rao, Pratima; Deobagkar, DN; Madyastha, KM

    1983-01-01

    A high-performance liquid chromatographic sepration method is described for the detection of 5-methylcytosine and 6-methyladenine in nucleic acid ext. The bases were sepd. on a Waters $C18 \\mu$ Bondapak column with a water: methanol acetic acid system. Effluents were monitored by UV absorption at 254 nm. The bases were estd. by peak heights which are proportional to the amts. of the individual bases. The method is rapid, sensitive, easy to perform and reproducible.

  20. Protein selectivity with immobilized metal ion-tacn sorbents: chromatographic studies with human serum proteins and several other globular proteins.

    Science.gov (United States)

    Jiang, W; Graham, B; Spiccia, L; Hearn, M T

    1998-01-01

    The chromatographic selectivity of the immobilized chelate system, 1,4,7-triazocyclononane (tacn), complexed with the borderline metal ions Cu2+, Cr3+, Mn2+, Co2+, Zn2+, and Ni2+ has been investigated with hen egg white lysozyme, horse heart cytochrome c, and horse skeletal muscle myoglobin, as well as proteins present in partially fractionated preparations of human plasma. The effects of ionic strength and pH of the loading and elution buffers on protein selectivities of these new immobilized metal ion affinity chromatographic (IMAC) systems have been examined. The results confirm that immobilized Mn;pl-tacn sorbents exhibit a novel type of IMAC behavior with proteins. In particular, the chromatographic properties of these immobilized M(n+)-tacn ligand systems were significantly different compared to the IMAC behavior observed with other types of immobilized tri- and tetradentate chelating ligands, such as iminodiacetic acid, O-phosphoserine, or nitrilotriacetic acid, when complexed with borderline metal ions. The experimental results have consequently been evaluated in terms of the additional contributions to the interactive processes mediated by effects other than solely the conventional lone pair Lewis soft acid-Lewis soft base coordination interactions, typically found for the IMAC of proteins with borderline and soft metal ions, such as Cu2+ or Ni2+.

  1. Effects of γ-irradiation on caprolactam level from multilayer PA-6 films for food packaging: Development and validation of a gas chromatographic method

    International Nuclear Information System (INIS)

    Araujo, Henrique Peres; Felix, Juliana Silva; Manzoli, Jose Eduardo; Padula, Marisa; Monteiro, Magali

    2008-01-01

    A gas chromatographic method to determine caprolactam in multilayer PA-6 films used for meat foodstuffs and cheese was developed and validated. A wide linear range (0.8-400 μg/ml), RSD≤4.1% and recovery higher than 90.0% were obtained for the chromatographic system, while precision and accuracy of the method showed RSD≤3.8%, recovery from 95.5-100.0% and LOQ of 32 μg/g. Irradiated (3, 7 and 12 kGy) and non-irradiated commercial films were analyzed. Most of them increased caprolactam levels with the increase of irradiation doses

  2. Effects of {gamma}-irradiation on caprolactam level from multilayer PA-6 films for food packaging: Development and validation of a gas chromatographic method

    Energy Technology Data Exchange (ETDEWEB)

    Araujo, Henrique Peres; Felix, Juliana Silva [Department of Food and Nutrition, School of Pharmaceutical Science, Sao Paulo State University, PO Box 502, 14801-902 Araraquara, SP (Brazil); Manzoli, Jose Eduardo [Nuclear and Energetic Research Institute (IPEN), Sao Paulo, SP (Brazil); Padula, Marisa [Packaging Technology Center/Food Technology Institute (CETEA/ITAL), Campinas, SP (Brazil); Monteiro, Magali [Department of Food and Nutrition, School of Pharmaceutical Science, Sao Paulo State University, PO Box 502, 14801-902 Araraquara, SP (Brazil)], E-mail: monteiro@fcfar.unesp.br

    2008-07-15

    A gas chromatographic method to determine caprolactam in multilayer PA-6 films used for meat foodstuffs and cheese was developed and validated. A wide linear range (0.8-400 {mu}g/ml), RSD{<=}4.1% and recovery higher than 90.0% were obtained for the chromatographic system, while precision and accuracy of the method showed RSD{<=}3.8%, recovery from 95.5-100.0% and LOQ of 32 {mu}g/g. Irradiated (3, 7 and 12 kGy) and non-irradiated commercial films were analyzed. Most of them increased caprolactam levels with the increase of irradiation doses.

  3. Gas chromatographic/mass spectrometric determination of carbon isotope composition in unpurified samples: methamphetamine example.

    Science.gov (United States)

    Low, I A; Liu, R H; Legendre, M G; Piotrowski, E G; Furner, R L

    1986-10-01

    A gas chromatograph/quadrupole mass spectrometer system, operated in electron impact/selected ion monitoring mode, is used to determine the intensity ratio of the m/z 59 and the m/z 58 ions of the [C3H8N]+ fragment derived from methamphetamine samples synthesized with varying amounts of 13C-labeled methylamine. Crude products are introduced into the gas chromatograph without prior cleanup. The ratios measured were in excellent agreement with those calculated. A change in 0.25% use of 13C-methylamine is sufficient for product differentiation. The feasibility of using isotope labeling and subsequent mass spectrometric isotope ratio measurement as the basis of a compound tracing mechanism is discussed. Specifically, if methamphetamine samples manufactured from legal sources are asked to incorporate distinct 13C compositions, their sources can be traced when samples are diverted into illegal channels. Samples derived from illicit preparations can also be traced if the manufacturers of a precursor (methylamine in this case) incorporate distinct 13C compositions in their products.

  4. Headspace gas chromatographic method for the measurement of difluoroethane in blood.

    Science.gov (United States)

    Broussard, L A; Broussard, A; Pittman, T; Lafferty, D; Presley, L

    2001-01-01

    To develop a gas chromatographic assay for the analysis of difluoroethane, a volatile substance, in blood and to determine assay characteristics including linearity, limit of quantitation, precision, and specificity. Referral toxicology laboratory Difluoroethane, a colorless, odorless, highly flammable gas used as a refrigerant blend component and aerosol propellant, may be abused via inhalation. A headspace gas chromatographic procedure for the identification and quantitation of difluoroethane in blood is presented. A methanolic stock standard prepared from pure gaseous difluoroethane was used to prepare whole blood calibrators. Quantitation of difluoroethane was performed using a six-point calibration curve and an internal standard of 1-propanol. The assay is linear from 0 to 115 mg/L including a low calibrator at 4 mg/L, the limit of quantitation. Within-run coefficients of variation at mean concentrations of 13.8 mg/L and 38.5 mg/L were 5.8% and 6.8% respectively. Between-run coefficients of variation at mean concentrations of 15.9 mg/L and 45.7 mg/L were 13.4% and 9.8% respectively. Several volatile substances were tested as potential interfering compounds with propane having a retention time identical to that of difluoroethane. This method requires minimal sample preparation, is rapid and reproducible, can be modified for the quantitation of other volatiles, and could be automated using an automatic sampler/injector system.

  5. Artificial neural network model for photosynthetic pigments identification using multi wavelength chromatographic data

    Science.gov (United States)

    Prilianti, K. R.; Hariyanto, S.; Natali, F. D. D.; Indriatmoko, Adhiwibawa, M. A. S.; Limantara, L.; Brotosudarmo, T. H. P.

    2016-04-01

    The development of rapid and automatic pigment characterization method become an important issue due to the fact that there are only less than 1% of plant pigments in the earth have been explored. In this research, a mathematical model based on artificial intelligence approach was developed to simplify and accelerate pigment characterization process from HPLC (high-performance liquid chromatography) procedure. HPLC is a widely used technique to separate and identify pigments in a mixture. Input of the model is chromatographic data from HPLC device and output of the model is a list of pigments which is the spectrum pattern is discovered in it. This model provides two dimensional (retention time and wavelength) fingerprints for pigment characterization which is proven to be more accurate than one dimensional fingerprint (fixed wavelength). Moreover, by mimicking interconnection of the neuron in the nervous systems of the human brain, the model have learning ability that could be replacing expert judgement on evaluating spectrum pattern. In the preprocessing step, principal component analysis (PCA) was used to reduce the huge dimension of the chromatographic data. The aim of this step is to simplify the model and accelerate the identification process. Six photosynthetic pigments i.e. zeaxantin, pheophytin a, α-carotene, β-carotene, lycopene and lutein could be well identified by the model with accuracy up to 85.33% and processing time less than 1 second.

  6. Similarity analyses of chromatographic herbal fingerprints: a review.

    Science.gov (United States)

    Goodarzi, Mohammad; Russell, Paul J; Vander Heyden, Yvan

    2013-12-04

    Herbal medicines are becoming again more popular in the developed countries because being "natural" and people thus often assume that they are inherently safe. Herbs have also been used worldwide for many centuries in the traditional medicines. The concern of their safety and efficacy has grown since increasing western interest. Herbal materials and their extracts are very complex, often including hundreds of compounds. A thorough understanding of their chemical composition is essential for conducting a safety risk assessment. However, herbal material can show considerable variability. The chemical constituents and their amounts in a herb can be different, due to growing conditions, such as climate and soil, the drying process, the harvest season, etc. Among the analytical methods, chromatographic fingerprinting has been recommended as a potential and reliable methodology for the identification and quality control of herbal medicines. Identification is needed to avoid fraud and adulteration. Currently, analyzing chromatographic herbal fingerprint data sets has become one of the most applied tools in quality assessment of herbal materials. Mostly, the entire chromatographic profiles are used to identify or to evaluate the quality of the herbs investigated. Occasionally only a limited number of compounds are considered. One approach to the safety risk assessment is to determine whether the herbal material is substantially equivalent to that which is either readily consumed in the diet, has a history of application or has earlier been commercialized i.e. to what is considered as reference material. In order to help determining substantial equivalence using fingerprint approaches, a quantitative measurement of similarity is required. In this paper, different (dis)similarity approaches, such as (dis)similarity metrics or exploratory analysis approaches applied on herbal medicinal fingerprints, are discussed and illustrated with several case studies. Copyright © 2013

  7. Chromatographic fingerprint similarity analysis for pollutant source identification

    International Nuclear Information System (INIS)

    Xie, Juan-Ping; Ni, Hong-Gang

    2015-01-01

    In the present study, a similarity analysis method was proposed to evaluate the source-sink relationships among environmental media for polybrominated diphenyl ethers (PBDEs), which were taken as the representative contaminants. Chromatographic fingerprint analysis has been widely used in the fields of natural products chemistry and forensic chemistry, but its application to environmental science has been limited. We established a library of various sources of media containing contaminants (e.g., plastics), recognizing that the establishment of a more comprehensive library allows for a better understanding of the sources of contamination. We then compared an environmental complex mixture (e.g., sediment, soil) with the profiles in the library. These comparisons could be used as the first step in source tracking. The cosine similarities between plastic and soil or sediment ranged from 0.53 to 0.68, suggesting that plastic in electronic waste is an important source of PBDEs in the environment, but it is not the only source. A similarity analysis between soil and sediment indicated that they have a source-sink relationship. Generally, the similarity analysis method can encompass more relevant information of complex mixtures in the environment than a profile-based approach that only focuses on target pollutants. There is an inherent advantage to creating a data matrix containing all peaks and their relative levels after matching the peaks based on retention times and peak areas. This data matrix can be used for source identification via a similarity analysis without quantitative or qualitative analysis of all chemicals in a sample. - Highlights: • Chromatographic fingerprint analysis can be used as the first step in source tracking. • Similarity analysis method can encompass more relevant information of pollution. • The fingerprints strongly depend on the chromatographic conditions. • A more effective and robust method for identifying similarities is required

  8. Diffusion and flow of water vapours in chromatographic Alumina gel

    International Nuclear Information System (INIS)

    Khan, M.; Shah, H. U.

    2005-01-01

    The kinetics of sorption of water vapours in chromatographic alumina gel was studied. Water vapours are adsorbed on the gel at temperature (15 degree C) at different constant relative pressure from 0.1-0.93 p/p. Rate constant, Effective diffusivities, Knudsen diffusivities and bulk diffusivities were determined through Fick type equation. Total pore volume is 0.498 cc g-1 and specific surface area comes to be 465 m2 g-1 as obtained by Gurvitsch rule and Kieselve's quantities respectively. An average pore radius (hydraulic) is 1.1x10/sub -7/ cm. The study of these quantities provide a strong basis for evaluating surface properties. (author)

  9. Gas chromatographic determination of impurities of inorganic compounds

    International Nuclear Information System (INIS)

    Drugov, Yu.S.

    1985-01-01

    Methods of concentration, separation, detection in gas chromatographic determination of impurities of inorganic compounds including low-boiling gases, reactive gases, organometallic compounds, free metals, anions, etc. are reviewed. Methods of reaction gas chromatography for determining reactive gases, water, anions, metal chelates are considered in detail as well as methods of reaction-sorption concentration and reaction gas extraction. The application of gas chromatograpny ior anaiysis of water and atmosphere contamination, for determination of impurities in highly pure solid substances and gases is described

  10. Large scale chromatographic separations using continuous displacement chromatography (CDC)

    International Nuclear Information System (INIS)

    Taniguchi, V.T.; Doty, A.W.; Byers, C.H.

    1988-01-01

    A process for large scale chromatographic separations using a continuous chromatography technique is described. The process combines the advantages of large scale batch fixed column displacement chromatography with conventional analytical or elution continuous annular chromatography (CAC) to enable large scale displacement chromatography to be performed on a continuous basis (CDC). Such large scale, continuous displacement chromatography separations have not been reported in the literature. The process is demonstrated with the ion exchange separation of a binary lanthanide (Nd/Pr) mixture. The process is, however, applicable to any displacement chromatography separation that can be performed using conventional batch, fixed column chromatography

  11. Chromatographic separation of alkaline phosphatase from dental enamel

    DEFF Research Database (Denmark)

    Moe, D; Kirkeby, S; Salling, E

    1989-01-01

    Alkaline phosphatase (AP) was prepared from partly mineralized bovine enamel by extraction in phosphate buffer, centrifugation and various chromatographic techniques. Chromatofocusing showed that the enamel enzyme possessed five isoelectric points at the acid pH level ranging from pH 5.7 to pH 4.......4. Three enzyme peaks were eluted using low pressure chromatography with a Bio-gel column. With a HPLC gel filtration column the separation of the enamel extract resulted in only one peak with AP activity. The fractions of this peak were used to produce an antibody against bovine AP....

  12. Chromatographic analysis and purification of multiply tritium-labelled eicosanoids

    International Nuclear Information System (INIS)

    Shevchenko, V.P.; Nagaev, I.Yu.; Myasoedov, N.F.

    1988-01-01

    A comparative study of different chromatographic techniques (gas-liquid (GLC), thin-layer (TLC), liquid (LC), high-pressure liquid (HPLC) chromatography) is presented. They were applied to the analysis and preparative purification of tritium-labelled eicosanoids with a molar radioactivity of 1.8-8.8 TBq/mmol, obtained by selective hydrogenation and by chemical or enzymic methods. The possibility of analyzing reaction mixtures and isolating individual multiply labelled eicosanoids with a chemical and radiochemical purity of 95-98% was demonstrated. Special features of HPLC for high molar radioactivity eicosanoids are considered. (author) 9 refs.; 6 tabs

  13. Gas chromatographic method fr determination of carbon in metallic uranium

    International Nuclear Information System (INIS)

    Nikol'skij, V.A.; Markov, V.K.; Evseeva, T.I.; Cherstvenkova, E.P.

    1983-01-01

    Gas chromatographic device to determine carbon in metal uranium is developed. Burnout unite, permitting to load in the burnout tube simultaneously quite a few (up to 20) weight amounts of materials to be burned is a characteristic feature of the device. As a result amendments for control experiment and determination limit are decreased. The time of a single determination is also reduced. Conditions of carbon burn out from metal uranium are studied and temperature and time of complete extraction of carbon in the form of dioxide from weight amount into gaseous phase are established

  14. Determination of hexachlorocyclohexane pesticide residues in wool fat by a combined high-performance liquid chromatographic-gas-liquid chromatographic method

    International Nuclear Information System (INIS)

    Ali, S.L.

    1978-01-01

    Beta- and gamma-hexachlorocyclohexane residues were determined in twelve wool fat samples by using a combined high-performance liquid chromatographic (HPLC)-gas-liquid chromatographic (GLC) method. After extraction and chromatographic clean-up on a silca-gel column, the sample was further purified by HPLC on a reversed-phase C-18 column with methanol as the mobile phase. The final determination was effected by GLC with a 1-mCi nickel-63 electron-capture detector. The analytical method was checked by addition of carbon-14-labelled lindane and measurement of the radioactivity in a liquid scintillation counter. (Auth.)

  15. Chromatographic Methods for the Analysis of Polyphenols in Wines

    Directory of Open Access Journals (Sweden)

    Medić-Šarić, M.

    2009-03-01

    Full Text Available Wine is an excellent source of various classes of polyphenols, including phenolic acids, flavonoids, and trihydroxystilbene resveratrol (Fig.1. Polyphenols play a major role in wine quality since they contribute to the sensory characteristics of wine, particularly color and astringency. A recent interest in these substances has been stimulated by abundant evidence of their beneficial effects on human health, such as anticarcinogenic, antiinflamatory and antimicrobial activities. Therefore, numerous studies have been performed in the attempt to analyze polyphenols in wine. This paper reviews the current advances in the determination of polyphenols in wine by the major chromatographic techniques such as thin-layer chromatography (TLC and high-performance liquid chromatography (HPLC.The great complexity of the polyphenolic content of wine and the difficulty in obtaining some of the standards usually require sample preparation before analysis. Two methods for sample preparation, liquid-liquid extraction and solid-phase extraction, are most commonly applied. Hydrolysis is applied frequently, but not exclusively, to remove the sugar moieties from glycosides.TLC on silica gel plates is useful for the rapid and low-cost separation and identification of the polyphenols present in wine (Fig. 2. Densitometric quantitative analysis of polyphenols in wine extracts is usually performed by scanning the TLC plates with UV light at wavelengths of 350–365 nm or 250–260 nm (Fig. 3. For the evaluation of the most efficient mobile phase and an optimal choice of the combination of two or more mobile phases, two methods may be applied: information theory and numerical taxonomy. HPLC currently represents the most popular technique for the analysis of polyphenols in wine. For this purpose, a reversed-phase HPLC method that uses gradient elution with binary elution system is usually employed. Routine detection is based on measurement of UV-Vis absorption with a diode

  16. OpenChrom: a cross-platform open source software for the mass spectrometric analysis of chromatographic data.

    Science.gov (United States)

    Wenig, Philip; Odermatt, Juergen

    2010-07-30

    Today, data evaluation has become a bottleneck in chromatographic science. Analytical instruments equipped with automated samplers yield large amounts of measurement data, which needs to be verified and analyzed. Since nearly every GC/MS instrument vendor offers its own data format and software tools, the consequences are problems with data exchange and a lack of comparability between the analytical results. To challenge this situation a number of either commercial or non-profit software applications have been developed. These applications provide functionalities to import and analyze several data formats but have shortcomings in terms of the transparency of the implemented analytical algorithms and/or are restricted to a specific computer platform. This work describes a native approach to handle chromatographic data files. The approach can be extended in its functionality such as facilities to detect baselines, to detect, integrate and identify peaks and to compare mass spectra, as well as the ability to internationalize the application. Additionally, filters can be applied on the chromatographic data to enhance its quality, for example to remove background and noise. Extended operations like do, undo and redo are supported. OpenChrom is a software application to edit and analyze mass spectrometric chromatographic data. It is extensible in many different ways, depending on the demands of the users or the analytical procedures and algorithms. It offers a customizable graphical user interface. The software is independent of the operating system, due to the fact that the Rich Client Platform is written in Java. OpenChrom is released under the Eclipse Public License 1.0 (EPL). There are no license constraints regarding extensions. They can be published using open source as well as proprietary licenses. OpenChrom is available free of charge at http://www.openchrom.net.

  17. Fundulus heteroclitus gonadotropins.5: Small scale chromatographic fractionation of pituitary extracts into components with different steroidogenic activities using homologous bioassays

    Directory of Open Access Journals (Sweden)

    Petrino Teresa R

    2004-03-01

    Full Text Available Abstract Fractionation and characterization of gonadotropins (GtH from Fundulus heteroclitus pituitary extracts were carried out using a biocompatible liquid chromatographic procedure (Pharmacia FPLC system. Chromatographic fractions were monitored for gonadotropic activities (induction of oocyte maturation and steroid production using homologous follicle bioassays in vitro. Size-exclusion chromatography eluted gonadotropic activity in one major protein peak (Mr ~ 30,000. Anion-exchange and hydrophobic-interaction chromatography (HIC yielded two distinct peaks of 17beta-estradiol (E2- and 17alpha-hydroxy,20beta-dihydroprogesterone (DHP-promoting activity with associated oocyte maturation. Two-dimensional chromatography (chromatofocusing followed by HIC resolved pituitary extracts into two active fractions; both induced E2 synthesis, but one was relatively poor in eliciting DHP and testosterone production. Thus, using homologous bioassays, at least two quantitatively different gonadotropic (steroidogenic activities: an E2-promoting gonadotropin (GtH I-like and a DHP-promoting gonadotropin (GtH II-like, which has a lower isoelectric point but greater hydrophobicity than the former, can be distinguished from F. heteroclitus pituitaries by a variety of chromatographic procedures. This study complements previous biochemical and molecular data in F. heteroclitus and substantiates the duality of GtH function in a multiple-spawning teleost.

  18. Separation and determination of high-carbon alcohols using method of column chromatographic and gas-chromatographic analysis

    International Nuclear Information System (INIS)

    Kang Zhongrong; Li Biping; Zeng Yongchang

    1988-01-01

    This paper describes the separation and determination of high-carbon alcohols from amine extractant by using the method of column chromatography of aluminium oxide and gas-chromatographic analysis. The total conent of high-carbon alcohols is determined by the method of column chromatography, while the components of the high-carbon alcohols and their relative contents are determined by the method of gas-chromatography. A simple reliable and practical method is provided for the analysis of high-carbon alcohol from the amine extractant in this paper

  19. Warping methods for spectroscopic and chromatographic signal alignment: A tutorial

    Energy Technology Data Exchange (ETDEWEB)

    Bloemberg, Tom G., E-mail: T.Bloemberg@science.ru.nl [Radboud University Nijmegen, Institute for Molecules and Materials, Heyendaalseweg 135, 6525 AJ Nijmegen (Netherlands); Radboud University Nijmegen, Education Institute for Molecular Sciences, Heyendaalseweg 135, 6525 AJ Nijmegen (Netherlands); Gerretzen, Jan; Lunshof, Anton [Radboud University Nijmegen, Institute for Molecules and Materials, Heyendaalseweg 135, 6525 AJ Nijmegen (Netherlands); Wehrens, Ron [Centre for Research and Innovation, Fondazione Edmund Mach, Via E. Mach, 1, 38010 San Michele all’Adige, TN (Italy); Buydens, Lutgarde M.C. [Radboud University Nijmegen, Institute for Molecules and Materials, Heyendaalseweg 135, 6525 AJ Nijmegen (Netherlands)

    2013-06-05

    Highlights: •The concepts of warping and alignment are introduced. •The most important warping methods are critically reviewed and explained. •Reference selection, evaluation and place of warping in preprocessing are discussed. •Some pitfalls, especially for LC–MS and similar data, are addressed. •Examples are provided, together with programming scripts to rework and extend them. -- Abstract: Warping methods are an important class of methods that can correct for misalignments in (a.o.) chemical measurements. Their use in preprocessing of chromatographic, spectroscopic and spectrometric data has grown rapidly over the last decade. This tutorial review aims to give a critical introduction to the most important warping methods, the place of warping in preprocessing and current views on the related matters of reference selection, optimization, and evaluation. Some pitfalls in warping, notably for liquid chromatography–mass spectrometry (LC–MS) data and similar, will be discussed. Examples will be given of the application of a number of freely available warping methods to a nuclear magnetic resonance (NMR) spectroscopic dataset and a chromatographic dataset. As part of the Supporting Information, we provide a number of programming scripts in Matlab and R, allowing the reader to work the extended examples in detail and to reproduce the figures in this paper.

  20. Chromatographic separation of Iodine species for environmental studies

    Energy Technology Data Exchange (ETDEWEB)

    Machado, E.C. [Universidade Federal Fluminense, Niteroi, RJ (Brazil). Dept. de Geoquimica]. E-mail: geoedin@vm.uff.br; Bellido, A.V.B. [Universidade Federal Fluminense, Niteroi, RJ (Brazil). Dept. de Fisico-Quimica]. E-mail: alf@risc2.rmn.uff.br; Bellido, L.F. [Comissao Nacional de Energia Nuclear (CNEN), Rio de Janeiro, RJ (Brazil)]. E-mail: lbellido@cnen.gov.br

    1999-07-01

    In this work a experimental method was developed to separate iodine species from environmental samples by using adsorption chromatography. The radionuclide {sup 123} I which has a half-life of 13 h and a convenient gamma ray of 159 keV was used to investigate a fast and efficient procedure for the separation of iodide and iodate ions in seawater from mangrove samples. The radio-iodine tracer technique is very useful, particularly in kinetic studies because it is easy to detect without any interference of concentration due to the small amount of tracer that is spiked. It is also possible to add two tracers (I-123 and I-131) in different oxidation states. Three chromatographic methods were studied: paper, thin layer and adsorption chromatography with silica and alumina. It was found that paper chromatography is very useful for checking the valence adjustment of the radioiodine species. Initially, several coefficient distributions of iodine species by adsorption in silica and alumina from NaOH, NaHCO{sub 3} and NaNO{sub 3} solutions (in the range 05. - 0.0001 m) were determined. The best separation so far was achieved by loading the sample (after adjusting the concentration to 0.1 NNaNO{sub 3}) into a chromatographic column with Al{sub 2} O{sub 3}. The iodide passes through the column and after washing the column, the iodate was removed by eluting with 1.0 M NaHCO{sub 3} solution. (author)

  1. Chromatographic separation of Iodine species for environmental studies

    International Nuclear Information System (INIS)

    Machado, E.C.

    1999-01-01

    In this work a experimental method was developed to separate iodine species from environmental samples by using adsorption chromatography. The radionuclide 123 I which has a half-life of 13 h and a convenient gamma ray of 159 keV was used to investigate a fast and efficient procedure for the separation of iodide and iodate ions in seawater from mangrove samples. The radio-iodine tracer technique is very useful, particularly in kinetic studies because it is easy to detect without any interference of concentration due to the small amount of tracer that is spiked. It is also possible to add two tracers (I-123 and I-131) in different oxidation states. Three chromatographic methods were studied: paper, thin layer and adsorption chromatography with silica and alumina. It was found that paper chromatography is very useful for checking the valence adjustment of the radioiodine species. Initially, several coefficient distributions of iodine species by adsorption in silica and alumina from NaOH, NaHCO 3 and NaNO 3 solutions (in the range 05. - 0.0001 m) were determined. The best separation so far was achieved by loading the sample (after adjusting the concentration to 0.1 NNaNO 3 ) into a chromatographic column with Al 2 O 3 . The iodide passes through the column and after washing the column, the iodate was removed by eluting with 1.0 M NaHCO 3 solution. (author)

  2. Ion Chromatographic Analyses of Sea Waters, Brines and Related Samples

    Directory of Open Access Journals (Sweden)

    Nataša Gros

    2013-06-01

    Full Text Available This review focuses on the ion chromatographic methods for the analyses of natural waters with high ionic strength. At the beginning a natural diversity in ionic composition of waters is highlighted and terminology clarified. In continuation a brief overview of other review articles of potential interest is given. A review of ion chromatographic methods is organized in four sections. The first section comprises articles focused on the determination of ionic composition of water samples as completely as possible. The sections—Selected Anions, Selected Cations and Metals—follow. The most essential experimental conditions used in different methods are summarized in tables for a rapid comparison. Techniques encountered in the reviewed articles comprise: direct determinations of ions in untreated samples with ion- or ion-exclusion chromatography, or electrostatic ion chromatography; matrix elimination with column-switching; pre-concentration with a chelation ion chromatography and purge-and-trap pre-concentration. Different detection methods were used: non-suppressed conductometric or suppressed conductometric, direct spectrometric or spectrometric after a post-column derivetization, and inductively coupled plasma in combination with optical emission or mass spectrometry.

  3. Similarity analyses of chromatographic herbal fingerprints: A review

    International Nuclear Information System (INIS)

    Goodarzi, Mohammad; Russell, Paul J.; Vander Heyden, Yvan

    2013-01-01

    Graphical abstract: -- Highlights: •Similarity analyses of herbal fingerprints are reviewed. •Different (dis)similarity approaches are discussed. •(Dis)similarity-metrics and exploratory-analysis approaches are illustrated. •Correlation and distance-based measures are overviewed. •Similarity analyses illustrated by several case studies. -- Abstract: Herbal medicines are becoming again more popular in the developed countries because being “natural” and people thus often assume that they are inherently safe. Herbs have also been used worldwide for many centuries in the traditional medicines. The concern of their safety and efficacy has grown since increasing western interest. Herbal materials and their extracts are very complex, often including hundreds of compounds. A thorough understanding of their chemical composition is essential for conducting a safety risk assessment. However, herbal material can show considerable variability. The chemical constituents and their amounts in a herb can be different, due to growing conditions, such as climate and soil, the drying process, the harvest season, etc. Among the analytical methods, chromatographic fingerprinting has been recommended as a potential and reliable methodology for the identification and quality control of herbal medicines. Identification is needed to avoid fraud and adulteration. Currently, analyzing chromatographic herbal fingerprint data sets has become one of the most applied tools in quality assessment of herbal materials. Mostly, the entire chromatographic profiles are used to identify or to evaluate the quality of the herbs investigated. Occasionally only a limited number of compounds are considered. One approach to the safety risk assessment is to determine whether the herbal material is substantially equivalent to that which is either readily consumed in the diet, has a history of application or has earlier been commercialized i.e. to what is considered as reference material. In order

  4. Similarity analyses of chromatographic herbal fingerprints: A review

    Energy Technology Data Exchange (ETDEWEB)

    Goodarzi, Mohammad [Department of Analytical Chemistry and Pharmaceutical Technology, Center for Pharmaceutical Research, Vrije Universiteit Brussel, Laarbeeklaan 103, B-1090 Brussels (Belgium); Russell, Paul J. [Safety and Environmental Assurance Centre, Unilever, Colworth Science Park, Sharnbrook, Bedfordshire MK44 1LQ (United Kingdom); Vander Heyden, Yvan, E-mail: yvanvdh@vub.ac.be [Department of Analytical Chemistry and Pharmaceutical Technology, Center for Pharmaceutical Research, Vrije Universiteit Brussel, Laarbeeklaan 103, B-1090 Brussels (Belgium)

    2013-12-04

    Graphical abstract: -- Highlights: •Similarity analyses of herbal fingerprints are reviewed. •Different (dis)similarity approaches are discussed. •(Dis)similarity-metrics and exploratory-analysis approaches are illustrated. •Correlation and distance-based measures are overviewed. •Similarity analyses illustrated by several case studies. -- Abstract: Herbal medicines are becoming again more popular in the developed countries because being “natural” and people thus often assume that they are inherently safe. Herbs have also been used worldwide for many centuries in the traditional medicines. The concern of their safety and efficacy has grown since increasing western interest. Herbal materials and their extracts are very complex, often including hundreds of compounds. A thorough understanding of their chemical composition is essential for conducting a safety risk assessment. However, herbal material can show considerable variability. The chemical constituents and their amounts in a herb can be different, due to growing conditions, such as climate and soil, the drying process, the harvest season, etc. Among the analytical methods, chromatographic fingerprinting has been recommended as a potential and reliable methodology for the identification and quality control of herbal medicines. Identification is needed to avoid fraud and adulteration. Currently, analyzing chromatographic herbal fingerprint data sets has become one of the most applied tools in quality assessment of herbal materials. Mostly, the entire chromatographic profiles are used to identify or to evaluate the quality of the herbs investigated. Occasionally only a limited number of compounds are considered. One approach to the safety risk assessment is to determine whether the herbal material is substantially equivalent to that which is either readily consumed in the diet, has a history of application or has earlier been commercialized i.e. to what is considered as reference material. In order

  5. Mixed-mode chromatographic matrices for the resolution of transfer ribonucleic acids

    NARCIS (Netherlands)

    Bischoff, Rainer; Mclaughlin, L.W.

    1984-01-01

    Modification of approximately 65% of the amine groups of an aminopropylsilyl bonded-phase silica high-performance liquid chromatographic anion exchanger (APS-Hypersil) with organic acids containing n-alkyl moieties of different chain lengths, results in mixed mode chromatographic matrices of varying

  6. Multiple chromatographic fingerprinting and its application to the quality control of herbal medicines

    Energy Technology Data Exchange (ETDEWEB)

    Fan Xiaohui [Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310027 (China); Cheng Yiyu [Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310027 (China)]. E-mail: chengyy@zju.edu.cn; Ye Zhengliang [Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310027 (China); Lin Ruichao [National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050 (China); Qian Zhongzhi [Committee of Chinese Pharmacopoeia, Beijing 100061 (China)

    2006-01-12

    Recently, chromatographic fingerprinting has become one of the most powerful approaches to quality control of herbal medicines. However, the performance of reported chromatographic fingerprinting constructed by single chromatogram sometimes turns out to be inadequate for complex herbal medicines, such as multi-herb botanical drug products. In this study, multiple chromatographic fingerprinting, which consists of more than one chromatographic fingerprint and represents the whole characteristics of chemical constitutions of the complex medicine, is proposed as a potential strategy in this complicated case. As a typical example, a binary chromatographic fingerprinting of 'Danshen Dropping Pill' (DSDP), the best-sold traditional Chinese medicine in China, was developed. First, two HPLC fingerprints that, respectively, represent chemical characteristics of depsides and saponins of DSDP were developed, which were used to construct binary chromatographic fingerprints of DSDP. Moreover, the authentication and validation of the binary fingerprints were performed. Then, a data-level information fusion method was employed to capture the chemical information encoded in two chromatographic fingerprints. Based on the fusion results, the lot-to-lot consistency and frauds can be determined either using similarity measure or by chemometrics approach. The application of binary chromatographic fingerprinting to consistency assessment and frauds detection of DSDP clearly demonstrated that the proposed method was a powerful approach to quality control of complex herbal medicines.

  7. Multiple chromatographic fingerprinting and its application to the quality control of herbal medicines

    International Nuclear Information System (INIS)

    Fan Xiaohui; Cheng Yiyu; Ye Zhengliang; Lin Ruichao; Qian Zhongzhi

    2006-01-01

    Recently, chromatographic fingerprinting has become one of the most powerful approaches to quality control of herbal medicines. However, the performance of reported chromatographic fingerprinting constructed by single chromatogram sometimes turns out to be inadequate for complex herbal medicines, such as multi-herb botanical drug products. In this study, multiple chromatographic fingerprinting, which consists of more than one chromatographic fingerprint and represents the whole characteristics of chemical constitutions of the complex medicine, is proposed as a potential strategy in this complicated case. As a typical example, a binary chromatographic fingerprinting of 'Danshen Dropping Pill' (DSDP), the best-sold traditional Chinese medicine in China, was developed. First, two HPLC fingerprints that, respectively, represent chemical characteristics of depsides and saponins of DSDP were developed, which were used to construct binary chromatographic fingerprints of DSDP. Moreover, the authentication and validation of the binary fingerprints were performed. Then, a data-level information fusion method was employed to capture the chemical information encoded in two chromatographic fingerprints. Based on the fusion results, the lot-to-lot consistency and frauds can be determined either using similarity measure or by chemometrics approach. The application of binary chromatographic fingerprinting to consistency assessment and frauds detection of DSDP clearly demonstrated that the proposed method was a powerful approach to quality control of complex herbal medicines

  8. High-pressure liquid chromatographic assay of Bay n 7133 in human serum.

    OpenAIRE

    Fasching, C E; Hughes, C E; Hector, R F; Peterson, L R

    1984-01-01

    A high-pressure liquid chromatographic method that includes a Sep-Pak (Waters Associates, Inc., Milford , Mass.) preparation of human serum was employed for the quantitative assay of Bay n 7133. Drug levels of 0.1 to 20 micrograms/ml could be detected. No interference from amphotericin B was found in the chromatographic analysis of Bay n 7133.

  9. Automated optimization and construction of chemometric models based on highly variable raw chromatographic data.

    Science.gov (United States)

    Sinkov, Nikolai A; Johnston, Brandon M; Sandercock, P Mark L; Harynuk, James J

    2011-07-04

    Direct chemometric interpretation of raw chromatographic data (as opposed to integrated peak tables) has been shown to be advantageous in many circumstances. However, this approach presents two significant challenges: data alignment and feature selection. In order to interpret the data, the time axes must be precisely aligned so that the signal from each analyte is recorded at the same coordinates in the data matrix for each and every analyzed sample. Several alignment approaches exist in the literature and they work well when the samples being aligned are reasonably similar. In cases where the background matrix for a series of samples to be modeled is highly variable, the performance of these approaches suffers. Considering the challenge of feature selection, when the raw data are used each signal at each time is viewed as an individual, independent variable; with the data rates of modern chromatographic systems, this generates hundreds of thousands of candidate variables, or tens of millions of candidate variables if multivariate detectors such as mass spectrometers are utilized. Consequently, an automated approach to identify and select appropriate variables for inclusion in a model is desirable. In this research we present an alignment approach that relies on a series of deuterated alkanes which act as retention anchors for an alignment signal, and couple this with an automated feature selection routine based on our novel cluster resolution metric for the construction of a chemometric model. The model system that we use to demonstrate these approaches is a series of simulated arson debris samples analyzed by passive headspace extraction, GC-MS, and interpreted using partial least squares discriminant analysis (PLS-DA). Copyright © 2011 Elsevier B.V. All rights reserved.

  10. Improvement in gradient ion chromatographic method for trace anions and organic acids in the PWR nuclear power plant

    International Nuclear Information System (INIS)

    Junlowjiraya, V.

    1989-01-01

    In a nuclear power plant the use of high quality water is very important to minimize corrosion and downtime of a plant system. To evaluate the quality of water the most widely used instrument is a conductivity analyzer. However, conductivity monitor is nonspecific, the conductivity signal cannot give information about what species or impurities are presented in the water, steam, and condensate. To determine these impurities qualitatively and quantitatively, the ion chromatography is an effective technique. This presentation demonstrates an improvement of gradient ion chromatographic technique to solve lithium interference in the borated samples. The data acquisition system and computer automation setup for the analysis are also discussed

  11. Measurement uncertainty of liquid chromatographic analyses visualized by Ishikawa diagrams.

    Science.gov (United States)

    Meyer, Veronika R

    2003-09-01

    Ishikawa, or cause-and-effect diagrams, help to visualize the parameters that influence a chromatographic analysis. Therefore, they facilitate the set up of the uncertainty budget of the analysis, which can then be expressed in mathematical form. If the uncertainty is calculated as the Gaussian sum of all uncertainty parameters, it is necessary to quantitate them all, a task that is usually not practical. The other possible approach is to use the intermediate precision as a base for the uncertainty calculation. In this case, it is at least necessary to consider the uncertainty of the purity of the reference material in addition to the precision data. The Ishikawa diagram is then very simple, and so is the uncertainty calculation. This advantage is given by the loss of information about the parameters that influence the measurement uncertainty.

  12. Chromatographic assay of degradation products of tributyl phosphate

    International Nuclear Information System (INIS)

    Tripathi, S.C.; Ramanujam, A.; Nadkarni, M.N.; Rao, K.A.; Bandyopadhyay, C.

    1985-01-01

    Gas-liquid chromatography (GLC) and thin-layer chromatographic (TLC) procedures have been developed for the estimation of monobutyl phosphoric acid (H 2 MBP) and dibutyl phosphoric acid (HDBP) present in tributyl phosphate (TBP). Less than one microgram of H 2 MBP and HDBP is visually detected on TLC plate made of silicagel-cellulose (1:1). HDBP as separated by TLC has been quantitatively estimated in the range of 40-260 μg using an indirect spectrophotometric method. GLC method using 10 percent (W/W) XE-60 column can be effectively used for the simultaneous determination of 0.25 percent W/V H 2 MBP and HDBP present in 30 percent TBP-n-dodecane. The sensitivity may be enhanced 100 fold by careful column conditioning and judicious control of operational parameters. (author)

  13. Modification of ion chromatograph for analyses of radioactive samples

    International Nuclear Information System (INIS)

    Curfman, L.L.; Johnson, S.J.

    1979-01-01

    In ion chromatographic analysis, the sample is injected through a sample loop onto an analytical column where separation occurs. The sample then passes through a suppressor column to remove or neutralize background ions. A flow-through conductivity cell is used as a detector. Depending upon column and eluent selection, ion chromatography can be used for anion or cation analyses. Ion chromatography has proven to be a versatile analytical tool for the analysis of anions in Hanford waste samples. These radioactive samples range from caustic high salt solutions to hydrochloric acid dissolutions of insoluble sludges. Instrument modifications which provide safe and convenient handling of these samples without lengthening analysis time or altering instrument performance are described

  14. Resolving and quantifying overlapped chromatographic bands by transmutation

    Science.gov (United States)

    Malinowski

    2000-09-15

    A new chemometric technique called "transmutation" is developed for the purpose of sharpening overlapped chromatographic bands in order to quantify the components. The "transmutation function" is created from the chromatogram of the pure component of interest, obtained from the same instrument, operating under the same experimental conditions used to record the unresolved chromatogram of the sample mixture. The method is used to quantify mixtures containing toluene, ethylbenzene, m-xylene, naphthalene, and biphenyl from unresolved chromatograms previously reported. The results are compared to those obtained using window factor analysis, rank annihilation factor analysis, and matrix regression analysis. Unlike the latter methods, the transmutation method is not restricted to two-dimensional arrays of data, such as those obtained from HPLC/DAD, but is also applicable to chromatograms obtained from single detector experiments. Limitations of the method are discussed.

  15. Gas chromatographic measurement in water-steam circuits

    International Nuclear Information System (INIS)

    Zschetke, J.; Nieder, R.

    1984-01-01

    A gas chromatographic technique for measurements in water-steam circuits, which has been well known for many years, has been improved by design modifications. A new type of equipment developed for special measuring tasks on nuclear engineering plant also has a general application. To date measurements have been carried out on the ''Otto Hahn'' nuclear powered ship, on the KNK and AVR experimental nuclear power plants at Karlsruhe and Juelich respectively and on experimental boiler circuits. The measurements at the power plants were carried out under different operating conditions. In addition measurements during the alkali operating mode and during combined cycle operation were carried out on the AVR reactor. It has been possible to draw new conclusion from the many measurements undertaken. (orig.) [de

  16. Study PWA8 resin for chromatographic uranium concentration

    International Nuclear Information System (INIS)

    Coceancigh, Herman; Ramella, J. L.; Marrero, Julieta; Jiménez Rebagliati, Raúl

    2013-01-01

    For many years nuclear industry have been using resins as filler of chromatographic columns. These methods are specific and give reliable results in different applications, for those reasons are extremely useful as separation process. Currently the nuclear industry is growing and this brings new issues such as the need of reduction of the amount of waste, the optimization of the production process and others that the chromatography could solve with great results. AMBERLITETM PWA8 resin is an anion exchange resin which can be used for the removal of uranium from drinking water. In addition to high exchange capacity, this resin has excellent physical stability and a wide range of pH in which is operational. With the idea of concentrating uranium from wastes solution as main goal we made different experiments to understand the AMBERLITETM PWA8 and obtain the most important characteristics like; pH working range; capacity; activation and elution procedures. These procedures were developed and optimized the capacity was determined using a batch experiment and we obtain that the maximum capacity is 882,5 U ug /resin gr at a pH of 4,2. Following on from these results chromatographic experiments were performed in which both were obtained the percentage of recovery and the concentration factor. The percent recovery (% R) calculated as the percentage ratio between the total mass and the load mass eluted (% R = eluted mass / total mass * 100) was 94% with a concentration factor of 5 times From these results it is intended to concentrate wastes solutions from the fuel cycle processes with two main goals: decreasing volume for storage and for future reusing of the uranium coming from production. (author)

  17. Chromatographic profiles of extractives from leaves of Eugenia uniflora

    Directory of Open Access Journals (Sweden)

    Isabelle C.F. Bezerra

    Full Text Available ABSTRACT Eugenia uniflora L., Myrtaceae, popularly known as “pitanga”, is used in traditional medicine due the properties attributed to its chemical content, these being mainly hydrolysable tannins and flavonoids. This study provides a qualitative and quantitative evaluation of chemical profile from leaves of E. uniflora. The HPLC analysis was carried out on a C18 column (4.6 mm × 250 mm, 5 µm by gradient elution with methanol and water (acidified with trifluoracetic acid; and silica gel Plates 60-F 254 with 10–12 µm and 5–6 µm particles, respectively for TLC and High HPTLC analysis. The chromatographic data obtained from HPLC, TLC and HPTLC presented bands and peaks related to flavonoids (myricitrin and derivatives and tannins (gallic and ellagic acids, which were observed from different samples. The chromatographic similarities enabled the building of a typical fingerprint for the herbal material. The similarity analysis of the sample data by Pearson correlation showed R values >0.9 among peaks (HPLC and bands (HPTLC. In addition, the analytical methodology developed by HPLC enabled the satisfactory quantification of marker substances [ellagic acid = 0.22% and 0.20% (m/m; gallic acid = 0.20% and 0.43%; myricitrin = 0.42 and 1.74% (m/m in herbal drug and crude extract, respectively]. The procedure was also validated in accordance with the assays required by Brazilian legislation. Thus, the HPTLC and HPLC methods developed in this study provide helpful and simple tools for the quality evaluation both qualitatively and quantitatively of raw materials and extractives from leaves of E. uniflora.

  18. Different Chromatographic Methods for Simultaneous Determination of Mefenamic Acid and Two of Its Toxic Impurities.

    Science.gov (United States)

    Morcoss, Martha M; Abdelwahab, Nada S; Ali, Nouruddin W; Elsaady, Mohammed T

    2017-08-01

    Two sensitive, accurate and precise chromatographic methods mentioned as TLC-densitometric method and RP-HPLC-DAD method, were developed and validated for the simultaneous determination of mefenamic acid (MEF) and its two toxic impurities, benzoic acid (BA) and 2,3-dimethylaniline (DMA). In the proposed TLC-densitometric method a developing system consisting of chloroform:acetone:acetic acid:ammonia solution(70:30:2:2, v/v/v/v) was used, TLC aluminum plates 60 F254 was used as a stationary phase and the separated bands were UV-scanned at 225 nm. While the proposed RP-HPLC-DAD method depended on chromatographic separation on C18 column using 0.05 M KH2PO4 buffer: acetonitrile (40:60, v/v) as a mobile phase at constant flow rate of 1 mL/min with UV detection at 225 nm. Linear relationships were obtained in the ranges of 0.3-2, 0.3-2 and 0.3-1.8 μg/band (for TLC-densitometric method) and in the ranges of 7-50, 10-50 and 7-50 μg/mL (for HPLC-DAD method) for MEF, BA and DMA, respectively. Factors affecting the developed methods have been studied and optimized. Moreover ,the proposed methods were successfully applied for determination of the studied drug in its pharmaceutical dosage form. The methods showed no significance difference when compared with the reported method using F-test and Student's-t test. The low of detection and quantization limits of the proposed methods get them suitable for quality control and stability studies of MEF in pharmaceutical formulation. The developed methods have advantages of being more selective and sensitive than the published methods. © The Author 2017. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  19. Different Spectrophotometric and Chromatographic Methods for Determination of Mepivacaine and Its Toxic Impurity.

    Science.gov (United States)

    Abdelwahab, Nada S; Fared, Nehal F; Elagawany, Mohamed; Abdelmomen, Esraa H

    2017-09-01

    Stability-indicating spectrophotometric, TLC-densitometric, and ultra-performance LC (UPLC) methods were developed for the determination of mepivacaine HCl (MEP) in the presence of its toxic impurity, 2,6-dimethylanaline (DMA). Different spectrophotometric methods were developed for the determination of MEP and DMA. In a dual-wavelength method combined with direct spectrophotometric measurement, the absorbance difference between 221.4 and 240 nm was used for MEP measurements, whereas the absorbance at 283 nm was used for measuring DMA in the binary mixture. In the second-derivative method, amplitudes at 272.2 and 232.6 nm were recorded and used for the determination of MEP and DMA, respectively. The developed TLC-densitometric method depended on chromatographic separation using silica gel 60 F254 TLC plates as a stationary phase and methanol-water-acetic acid (9 + 1 + 0.1, v/v/v) as a developing system, with UV scanning at 230 nm. The developed UPLC method depended on separation using a C18 column (250 × 4.6 mm id, 5 μm particle size) as a stationary phase and acetonitrile-water (40 + 60, v/v; pH 4 with phosphoric acid) as a mobile phase at a flow rate of 0.4 mL/min, with UV detection at 215 nm. The chromatographic run time was approximately 1 min. The proposed methods were validated with respect to International Conference on Harmonization guidelines regarding precision, accuracy, ruggedness, robustness, and specificity.

  20. Comparison of twin-cell centrifugal partition chromatographic columns with different cell volume.

    Science.gov (United States)

    Goll, Johannes; Audo, Gregoire; Minceva, Mirjana

    2015-08-07

    Two twin-cell centrifugal partition chromatographic columns (SCPC 250 and SCPE-250-BIO, Armen Instrument, France) with the same column volume but different cell size and number were compared in terms of stationary phase retention and column efficiency. The columns were tested with two types of solvent systems: a commonly used organic solvent based biphasic system from the ARIZONA solvent system family and a polymer/salt based aqueous two phase system (ATPS). The efficiency of the columns was evaluated by pulse injection experiments of two benzenediols (pyrocatechol and hydroquinone) in the case of the ARIZONA system and a protein mixture (myoglobin and lysozyme) in the case of the ATPS. As result of high stationary phase retention, the column with the lower number of larger twin-cells (SCPE-250-BIO) is suitable for protein separations using ATPS. On the other hand, due to higher column efficiency, the column with the greater number of smaller cells (SCPC 250) is superior for batch elution separations performed with standard liquid-liquid chromatography organic solvent based biphasic systems. Copyright © 2015 Elsevier B.V. All rights reserved.