WorldWideScience

Sample records for ty3 nuclear entry

  1. Determinants of Genomic RNA Encapsidation in the Saccharomyces cerevisiae Long Terminal Repeat Retrotransposons Ty1 and Ty3

    Directory of Open Access Journals (Sweden)

    Katarzyna Pachulska-Wieczorek

    2016-07-01

    Full Text Available Long-terminal repeat (LTR retrotransposons are transposable genetic elements that replicate intracellularly, and can be considered progenitors of retroviruses. Ty1 and Ty3 are the most extensively characterized LTR retrotransposons whose RNA genomes provide the template for both protein translation and genomic RNA that is packaged into virus-like particles (VLPs and reverse transcribed. Genomic RNAs are not divided into separate pools of translated and packaged RNAs, therefore their trafficking and packaging into VLPs requires an equilibrium between competing events. In this review, we focus on Ty1 and Ty3 genomic RNA trafficking and packaging as essential steps of retrotransposon propagation. We summarize the existing knowledge on genomic RNA sequences and structures essential to these processes, the role of Gag proteins in repression of genomic RNA translation, delivery to VLP assembly sites, and encapsidation.

  2. Characterization of active reverse transcriptase and nucleoprotein complexes of the yeast retrotransposon Ty3 in vitro.

    Science.gov (United States)

    Cristofari, G; Gabus, C; Ficheux, D; Bona, M; Le Grice, S F; Darlix, J L

    1999-12-17

    Human immunodeficiency virus (HIV) and the distantly related yeast Ty3 retrotransposon encode reverse transcriptase (RT) and a nucleic acid-binding protein designated nucleocapsid protein (NCp) with either one or two zinc fingers, required for HIV-1 replication and Ty3 transposition, respectively. In vitro binding of HIV-1 NCp7 to viral 5' RNA and primer tRNA(3)(Lys) catalyzes formation of nucleoprotein complexes resembling the virion nucleocapsid. Nucleocapsid complex formation functions in viral RNA dimerization and tRNA annealing to the primer binding site (PBS). RT is recruited in these nucleoprotein complexes and synthesizes minus-strand cDNA initiated at the PBS. Recent results on yeast Ty3 have shown that the homologous NCp9 promotes annealing of primer tRNA(i)(Met) to a 5'-3' bipartite PBS, allowing RNA:tRNA dimer formation and initiation of cDNA synthesis at the 5' PBS (). To compare specific cDNA synthesis in a retrotransposon and HIV-1, we have established a Ty3 model system comprising Ty3 RNA with the 5'-3' PBS, primer tRNA(i)(Met), NCp9, and for the first time, highly purified Ty3 RT. Here we report that Ty3 RT is as active as retroviral HIV-1 or murine leukemia virus RT using a synthetic template-primer system. Moreover, and in contrast to what was found with retroviral RTs, retrotransposon Ty3 RT was unable to direct cDNA synthesis by self-priming. We also show that Ty3 nucleoprotein complexes were formed in vitro and that the N terminus of NCp9, but not the zinc finger, is required for complex formation, tRNA annealing to the PBS, RNA dimerization, and primer tRNA-directed cDNA synthesis by Ty3 RT. These results indicate that NCp9 chaperones bona fide cDNA synthesis by RT in the yeast Ty3 retrotransposon, as illustrated for NCp7 in HIV-1, reinforcing the notion that Ty3 NCp9 is an ancestor of HIV-1 NCp7.

  3. Diversity and evolution of Ty1-copia and Ty3-gypsy retroelements in the non-photosynthetic flowering plants Orobanche and Phelipanche (Orobanchaceae).

    Science.gov (United States)

    Park, Jeong-Mi; Schneeweiss, Gerald M; Weiss-Schneeweiss, Hanna

    2007-01-31

    We present the first study on the diversity and evolution of Ty1-copia and Ty3-gypsy retroelements in a group of non-photosynthetic flowering plants. To this end partial sequences of the reverse transcriptase (rt) gene were obtained from 20 clones for each retroelement type from seven and six accessions of Orobanche and Phelipanche (Orobanchaceae), respectively. Overall sequence similarity is higher in Ty3-gypsy elements than in Ty1-copia elements in agreement with the results from other angiosperm groups. Higher sequence diversity and stronger phylogenetic structure, especially of Ty1-copia sequences, in Orobanche species compared to Phelipanche species support the previously suggested hypothesis (based on karyological and cytological data) that genomes of Orobanche species are more dynamic than those of Phelipanche species. No evidence was found for intraspecific differences of retroelement diversity nor for differences between pest taxa and their putative wild relatives, e.g., O. crenata and O. owerini. The occurrence of a few sequences from Phelipanche species in clades otherwise comprising sequences from Orobanche species might be due to horizontal gene transfer, but the alternative of vertical transmission cannot be rejected unambiguously.

  4. Työterveyshuolto esimiestyön psykososiaalisen työhyvinvoinnin tukena

    OpenAIRE

    Jeskanen, Jemina

    2014-01-01

    Hyvinvoiva esimies on keskeinen tekijä työntekijöiden ja työyhteisöjen hyvinvoinnille sekä työn tuloksellisuudelle. Muuttuvassa työelämässä esimiestyön vaatimukset kasvavat ja henkinen kuormittavuus lisääntyy. Esimiestyön merkitystä työntekijöiden työhyvinvointiin on tutkittu lukuisissa tutkimuksissa. Hoitoalan esimiesten työhyvinvointiin liittyvää tutkimus- ja kehittämistoimintaa on tehty kuitenkin suhteellisen vähän. Opinnäytetyön tarkoituksena oli kehittää työterveyshuollon palveluja ...

  5. Työpaikkavalmentaja osatyökykyisen henkilön työllistymisen tukena : työpaikkavalmentajien ja heidän esimiesten näkemyksiä työpaikkavalmentajuudesta

    OpenAIRE

    Leinonen, Kristiina

    2014-01-01

    Opinnäytetyössä tarkastelen työpaikkavalmentajuutta osatyökykyisen henkilön työllistymisen tukena työpaikkavalmentajina toimineiden henkilöiden ja heidän esimiestensä näkökulmasta. Aspa-säätiön kehittämisprojekteissa on kehitetty työpaikkavalmentajamallia osana osatyökykyisten tai muuten vaikeassa työmarkkina-asemassa olevien henkilöiden työllistymisen tukemista. Opinnäytetyön tutkimuskysymyksiä ovat: Millaisia kokemuksia työpaikkavalmentajilla ja heidän esimiehillään on työpaikkavalmentajuud...

  6. Osapäiväetätyön vaikutukset työhyvinvointiin Kevassa

    OpenAIRE

    Pylvänäinen, Katja

    2017-01-01

    Opinnäytetyön tarkoituksena oli selvittää vaikuttaako osapäiväetätyö työntekijän stressitasoon, parantaako osapäiväetätyö työntekijän vaikutusmahdollisuuksia omaan työhön, parantaako osapäiväetätyö työntekijän elämänhallintaa ja työn hallintaa, vaikuttaako osapäiväetätyö työtyytyväisyyteen, miten osapäiväetätyö vaikuttaa työntekijän työn ja vapaa-ajan yhteensovittamiseen, sekä miten muiden työntekijöiden mielipiteet ja kokemukset osapäiväetätyöstä vaikuttavat osapäiväetätyöntekijän työhyvinvo...

  7. The yeast Ty3 retrotransposon contains a 5'-3' bipartite primer-binding site and encodes nucleocapsid protein NCp9 functionally homologous to HIV-1 NCp7.

    Science.gov (United States)

    Gabus, C; Ficheux, D; Rau, M; Keith, G; Sandmeyer, S; Darlix, J L

    1998-08-17

    Retroviruses, including HIV-1 and the distantly related yeast retroelement Ty3, all encode a nucleoprotein required for virion structure and replication. During an in vitro comparison of HIV-1 and Ty3 nucleoprotein function in RNA dimerization and cDNA synthesis, we discovered a bipartite primer-binding site (PBS) for Ty3 composed of sequences located at opposite ends of the genome. Ty3 cDNA synthesis requires the 3' PBS for primer tRNAiMet annealing to the genomic RNA, and the 5' PBS, in cis or in trans, as the reverse transcription start site. Ty3 RNA alone is unable to dimerize, but formation of dimeric tRNAiMet bound to the PBS was found to direct dimerization of Ty3 RNA-tRNAiMet. Interestingly, HIV-1 nucleocapsid protein NCp7 and Ty3 NCp9 were interchangeable using HIV-1 and Ty3 RNA template-primer systems. Our findings impact on the understanding of non-canonical reverse transcription as well as on the use of Ty3 systems to screen for anti-NCp7 drugs.

  8. Hydrogeology of the interstream area between Ty Ty Creek and Ty Ty Creek tributary near Plains, Georgia

    Science.gov (United States)

    Stewart, Lisa M.; Hicks, David W.

    1996-01-01

    This report is part of an interdisciplinary effort to identify and describe processes that control movement and fate of selected fertilizers and pesticides in the surface and subsurface environments in the Fall Line Hills district of the Georgia Coastal Plain physiographic province. This report describes the hydrogeology of the interstream area between Ty Ty Creek and it's tributary near Plains, Sumter County, Georgia. Geologic units of interest to this study are, in ascending order, (1) the Tuscahoma Formation, a bluish gray, silty clay; (2) the Tallahatta Formation, a fine-to-coarse, poorly sorted quartz sand that is divided into an upper and lower unit; and (3) the undifferentiated overburden, which consists of fine to medium poorly sorted sand, silt and clay. Continuous-core samples indicate that the unsaturated zone includes the undifferentiated overburden and the upper unit of the Tallahatta Formation, and attains a maximum thickness of about 52 feet (ft) in the southern part of the study area. The Claiborne aquifer in the study area consists of the lower unit of the Tallahatta Formation and ranges in thickness from 3 ft near Ty Ty Creek tributary to about 20 ft in the upland divide area. It is confined below by the clayey sediments of the Tuscahoma Formation. The Claiborne aquifer in the study area generally is confined above by an extensive clay layer that is the base if the upper unit of the Tallahatta Formation. Fluctuations in the amount of vertical recharge to the aquifer result in areal and temporal changes in aquifer conditions from confined to unconfined in parts of the study area. Hydraulic conductivity of the aquifer ranges from 3.5 to 7 feet per day. The transmissivity of the aquifer is approximately 50 feet squared per day. Water-level data indicate the potentiometric surface slopes to the south, southeast, and southwest with a gradient of about 87 to 167 feet per mile. The shape of the potentiometric surface and the direction of groundwater flow

  9. The Mediator co-activator complex regulates Ty1 retromobility by controlling the balance between Ty1i and Ty1 promoters.

    Science.gov (United States)

    Salinero, Alicia C; Knoll, Elisabeth R; Zhu, Z Iris; Landsman, David; Curcio, M Joan; Morse, Randall H

    2018-02-01

    The Ty1 retrotransposons present in the genome of Saccharomyces cerevisiae belong to the large class of mobile genetic elements that replicate via an RNA intermediary and constitute a significant portion of most eukaryotic genomes. The retromobility of Ty1 is regulated by numerous host factors, including several subunits of the Mediator transcriptional co-activator complex. In spite of its known function in the nucleus, previous studies have implicated Mediator in the regulation of post-translational steps in Ty1 retromobility. To resolve this paradox, we systematically examined the effects of deleting non-essential Mediator subunits on the frequency of Ty1 retromobility and levels of retromobility intermediates. Our findings reveal that loss of distinct Mediator subunits alters Ty1 retromobility positively or negatively over a >10,000-fold range by regulating the ratio of an internal transcript, Ty1i, to the genomic Ty1 transcript. Ty1i RNA encodes a dominant negative inhibitor of Ty1 retromobility that blocks virus-like particle maturation and cDNA synthesis. These results resolve the conundrum of Mediator exerting sweeping control of Ty1 retromobility with only minor effects on the levels of Ty1 genomic RNA and the capsid protein, Gag. Since the majority of characterized intrinsic and extrinsic regulators of Ty1 retromobility do not appear to effect genomic Ty1 RNA levels, Mediator could play a central role in integrating signals that influence Ty1i expression to modulate retromobility.

  10. Toimijuus kuntoutuslaitoksen työhyvinvointiasiakkailla : case Peurunka

    OpenAIRE

    Joki, Riikka

    2013-01-01

    TIIVISTELMÄ Toimijuus kuntoutuslaitoksen työhyvinvointiasiakkailla Riikka Joki Fysioterapian pro gradu -tutkielma Jyväskylän yliopisto , Liikuntatieteellinen tiedekunta, Terveystieteiden laitos Kevät 2013 70 sivua, 3 liitettä Työelämä on muuttunut suorituskeskeiseksi, nopeasti muuttuvaksi ja lyhytjänteiseksi projektityöksi. Ihmissuhdeammateissa toimivilta vaaditaan stressinsietoa ja monipuolista sosiaalista ja psykologista osaamista. Työntekijöiden työkykyvyn ja työhyvinvoinnin säilymis...

  11. Työhyvinvointi päiväkodissa : henkilökunnan keinoja omaan työhyvinvointiin ja työssä jaksamiseen

    OpenAIRE

    Kalenius, Taina

    2013-01-01

    Opinnäytetyöni tavoitteena on selvittää yhden Oulun kaupungin päiväkodin työntekijöiden työssä jaksamisen keinoja. Tarkoituksenani on selvittää sellaisia keinoja, joihin työntekijät itse voivat vaikuttaa. Lisäksi tarkoituksenani on selvittää päiväkodin työntekijöiden työstä palautumisen keinoja. Lähestyn aihetta positiivisesta näkökulmasta, keskittymällä voimavaraistaviin keinoihin. Opinnäytetyöni teoreettisena viitekehyksenä on työhyvinvoinnin viisi porrasta. Sovellan teoriaa siltä osin, ...

  12. Työturvallisuuden parantaminen rikastamon kunnossapidossa

    OpenAIRE

    Kaisanlahti, S. (Santeri)

    2017-01-01

    Tiivistelmä Diplomityön tavoitteena on parantaa työturvallisuutta Agnico Eagle Finland Oy:n Kittilän kaivoksen rikastamon kunnossapidossa. Työn tilaus tuli kaivoksen työturvallisuusosastolta. Syynä työn tilaamiseen oli rikastamon kunnossapidossa aikaisemmin sattuneet tapaturmat ja rikastamon alueella sijaitsevat haastavat työkohteet. Työturvallisuus on...

  13. Hoitotyön opettajien työhyvinvointi ja työnohjaus sen kehittämismenetelmänä

    OpenAIRE

    Kuorikoski, Kati

    2013-01-01

    Työurien pidentäminen, väestön ikääntyminen, työhyvinvointi ja työkyvyn edistäminen ovat päivittäin esillä yhteiskunnallisessa keskustelussa. Tutkimustulokset viittaavat siihen, että hyviä työvuosia lisätään parhaiten edistämällä työkykyä jo työuran varhaisissa vaiheissa. Tulevana terveydenhoitajana ja työnohjaajana näen tärkeänä pohtia työhyvinvointiin vaikuttavia tekijöitä ja työnohjauksen mahdollisuuksia osana työn ja työhyvinvoinnin kokonaisvaltaista kehittämistä. Tutkimuksen tarkoitu...

  14. Työhyvinvoinnin kehittäminen työhyvinvointisuunnitelman avulla Tukipiste Akussa

    OpenAIRE

    Forsman, Linda

    2011-01-01

    Laurea-ammattikorkeakoulu Tiivistelmä Laurea Hyvinkää Sosiaalialan koulutusohjelma Forsman Linda Työhyvinvoinnin kehittäminen työhyvinvointisuunnitelman avulla Tukipiste Akussa. Vuosi 2011 Sivumäärä 69 Opinnäytetyössäni työelämän yhteistyötaho on kuvitteellisesti nimetyn Onnentuulet ry:n yksikkö Tukipiste Akku. Organisaatiosta käytetään fiktiivistä nimeä toiminnanjohtajan toiveesta, jota hän perustelee hankintalain mukanaan tuomalla markki...

  15. Nuclear entry of poliovirus protease-polymerase precursor 3CD: implications for host cell transcription shut-off

    International Nuclear Information System (INIS)

    Sharma, Rakhi; Raychaudhuri, Santanu; Dasgupta, Asim

    2004-01-01

    Host cell transcription mediated by all three RNA polymerases is rapidly inhibited after infection of mammalian cells with poliovirus (PV). Both genetic and biochemical studies have shown that the virus-encoded protease 3C cleaves the TATA-binding protein and other transcription factors at glutamine-glycine sites and is directly responsible for host cell transcription shut-off. PV replicates in the cytoplasm of infected cells. To shut-off host cell transcription, 3C or a precursor of 3C must enter the nucleus of infected cells. Although the 3C protease itself lacks a nuclear localization signal (NLS), amino acid sequence examination of 3D identified a potential single basic type NLS, KKKRD, spanning amino acids 125-129 within this polypeptide. Thus, a plausible scenario is that 3C enters the nucleus in the form of its precursor, 3CD, which then generates 3C by auto-proteolysis ultimately leading to cleavage of transcription factors in the nucleus. Using transient transfection of enhanced green fluorescent protein (EGFP) fusion polypeptides, we demonstrate here that both 3CD and 3D are capable of entering the nucleus in PV-infected cells. However, both polypeptides remain in the cytoplasm in uninfected HeLa cells. Mutagenesis of the NLS sequence in 3D prevents nuclear entry of 3D and 3CD in PV-infected cells. We also demonstrate that 3CD can be detected in the nuclear fraction from PV-infected HeLa cells as early as 2 h postinfection. Significant amount of 3CD is found associated with the nuclear fraction by 3-4 h of infection. Taken together, these results suggest that both the 3D NLS and PV infection are required for the entry of 3CD into the nucleus and that this may constitute a means by which viral protease 3C is delivered into the nucleus leading to host cell transcription shut-off

  16. Työntekijä aktiivisena työhyvinvoinnin edistäjänä tulkkauspalveluyrityksessä

    OpenAIRE

    Pakkala, Pigga-Maiju

    2013-01-01

    Kehittämistyön tilaajana oli Viittomakielialan Osuuskunta Via. Työn tavoitteena oli selvittää Viittomakielialan Osuuskunta Vian kuukausipalkkaisten tulkkien työhyvinvoinnin tekijöitä ja miten työntekijä itse pystyy aktiivisesti edistämään omaa ja työyhteisön hyvinvointia. Kehittämistyö toteutettiin yrityksen työhyvinvointihankkeessa keräten aineistoa samanaikaisesti hankkeelle kuin kehittämistyölle. Kehittämistyön menetelmänä oli realistinen evaluaatio, tarkemmin sanottuna prosessievaluaa...

  17. Työmotivaatiotutkimus : Case K-supermarket Petonen

    OpenAIRE

    Föhr, Virpi

    2013-01-01

    Opinnäytetyön tarkoituksena oli selvittää K-supermarket Petosen henkilökunnan työmotivaatio ja siihen vaikuttavia tekijöitä. Työmotivaatiotutkimus koostui seuraavista aihe-alueista: työmotivaatio, työn ominaisuudet, työilmapiiri/ työyhteisö, palkitseminen ja esimiestyö. Näitä aihe-alueita koskevien kysymysten pohjalta luotiin kokonaiskuva siitä, millainen henkilökunnan työmotivaatio oli tutkimus hetkellä ja miten nämä motivaatioon vaikuttavat asiat koetaan. Tutkimuksen tavoitteena oli myös se...

  18. Työpaikkakiusaaminen - sen seuraukset ja selviytymiskeinot

    OpenAIRE

    Päivärinta, Salla

    2014-01-01

    Opinnäytetyöni aihe on työpaikkakiusaaminen. Kiinnostus aihetta kohtaan syntyi omista kokemuksistani työpaikalla ja siitä, että olen ollut mukana luottamushenkilötoiminnassa useita vuosia. Tavoitteena on avata työpaikkakiusaamista ilmiönä, ja millaisia vakavia vaikutuksia sillä on niin työntekijään, hänen läheisiinsä, työpaikkaan ja koko yhteiskuntaan. Selvitän, mikä on ja mikä ei ole työpaikkakiusaamista ja epäasiallista häirintää. Kuvaan sitä, millaiseksi kiusaaja työpaikalla koetaan. Tutki...

  19. Työhyvinvoinnin johtaminen kauneudenhoitoalalla

    OpenAIRE

    Mäkisalo, Heli

    2014-01-01

    Tämä opinnäytetyö on kirjallisuuteen perustuva työ, jossa tarkastelun kohteena olivat työhy-vinvoinnin johtamisen keinot niin ylimmän johdon, lähiesimiehen kuin henkilöstön osalta. Tutkimusaineistona olivat johtamisesta, työhyvinvoinnista sekä tiimityöskentelystä käsittelevät kirjat ja julkaisut. Työssä tarkasteltiin johtamista myös kauneudenhoitoalan näkökulmasta, sillä alalla vallitsee joitakin erityispiirteitä, kuten pienyrittäjyys ja naisvaltaisuus. Nämä seikat tuovat omat haasteensa ja m...

  20. Työterveys- ja työturvallisuusjohtamisjärjestelmä : Case Tapojärvi Oy

    OpenAIRE

    Honkanen, Päivi

    2011-01-01

    Honkanen, Päivi. 2011. Työterveys- ja työturvallisuusjohtamisjärjestelmä. Case Tapo-järvi Oy. Opinnäytetyö. Kemi-Tornion ammattikorkeakoulu. Kaupan ja kulttuurin toimiala. Sivuja 33. Tämän opinnäytetyön tavoitteena on selvittää Tapojärvi Oy:n työterveys- ja työturvalli-suusjohtamisjärjestelmän nykytilaa ja arvioida kuinka se vastaa standardin OHSAS 18001:2007 vaatimuksia. Opinnäytetyö koostuu teoriaosuudesta ja empiirisestä tutkimuksesta. Teoriaosassa käsit-telen turvallisuusjohtamis...

  1. Työhyvinvointi varhaiskasvatustyössä

    OpenAIRE

    Penttilä, Anu

    2017-01-01

    Tutkimuksessani kartoitetaan helsinkiläisten varhaiskasvatuksen työntekijöiden kokemuksia työhyvinvoinnistaan. Sekä työhyvinvointi että varhaiskasvatukseen kohdistuvat muutokset puhuttavat tällä hetkellä paljon yhteiskunnallisessa keskustelussa. Aiempien tutkimusten perusteella työhyvinvointi koostuu monesta eri tekijästä. Työhyvinvointia ilmiönä on tutkittu paljon, muttei vielä laajemmin varhaiskasvatusalalla. Tutkimukseni pyrkii selvittämään, millaisena varhaiskasvatuksen henkilökunta kokee...

  2. Liiku, lepää, keskustele - matkalla työhyvinvointiin

    OpenAIRE

    Luukkonen, Päivi; Vahalahti, Tanja

    2016-01-01

    Työhyvinvoinnilla on suuri merkitys työssä jaksamisessa. Työssä jaksamiseen vaikutta-vat monet seikat, kuten arvot, elintavat, riittävä lepo ja henkinen hyvinvointi. Työkykyä voidaan seurata, hallita ja edistää monin eri keinoin. Johtamisella on suuri merkitys työntekijöiden työhyvinvoinnissa. Opinnäytetyömme tarkoituksena oli edistää Pkssk:n Honkalampi-keskuksen henkilökunnan työhyvinvointia tykytoiminnan avulla, painottaen työturvallisuutta, työssä jaksamiseen vaikuttavaa liikuntaa ja ...

  3. Hoitajien kokema työturvallisuus Martinlaakson terveysasemalla

    OpenAIRE

    Kulppi, Miia; Koponen, Aleksi

    2009-01-01

    Uhka- ja väkivaltatilanteet ovat työelämän kasvava ongelma. Väkivalta on yleistä varsinkin terveydenhuoltoalalla. Työpaikkaväkivalta on tapahtuma, jossa henkilöä loukataan sanallisesti, uhataan tai pahoinpidellään työssä. Se voi vaarantaa uhrin turvallisuuden, hyvinvoinnin ja terveyden. Työturvallisuutta tutkitaankin jatkuvasti, ja sen kehittämiseen kiinnitetään työpaikoilla entistä enemmän huomiota. Tämän opinnäytetyön tavoitteena on kuvata Martinlaakson terveysaseman hoitohenkilökunnan ...

  4. Työn ja vapaa-ajan tasapaino

    OpenAIRE

    Yoshida, Teemu

    2013-01-01

    Tämän opinnäytetyön aiheena oli työn ja vapaa-ajan tasapainon hallinta. Työssä paneuduttiin työn ja vapaa-ajan tasapainon määrittelyn vaikeuteen sekä tasapainon löytämisen mukanaan tuomiin hyötyihin. Tavoitteena oli selvittää, mitkä tekijät vaikuttavat työntekijän elämän tasapainoon. Tutkimus on rajattu organisaation näkökulmasta niihin tunnusmerkkeihin ja käytäntöihin, jotka määrittelevät perheystävällisen organisaatiokulttuurin unohtamatta kuitenkaan yksineläviä työntekijöitä. Työntekijän ...

  5. Työhyvinvointia edistävä toimistorakennuksen sisustussuunnitelma

    OpenAIRE

    Eklund, Hanna

    2011-01-01

    Tänä päivänä puhutaan paljon työuupumuksesta ja työstä aiheutuvasta stressistä. Näiden tekijöiden taustalla on usein kiire tai pelko työpaikan menettämisestä. Pelkoon pyritään vaikuttamaan hyvällä työntekijän ja esimiehen välisellä luottamussuhteella, mutta ennen kaikkea työn tehokkuudella. Työn tehokkuus lisää työn tuottavuutta, mikä vaikuttaa yrityksen menestykseen. Kun yrityksellä menee hyvin, on työntekijöiden irtisanominen harvinaisempaa kuin huonosti tuottavalla yrityksellä. Työntehokku...

  6. Automated entry control system for nuclear facilities

    International Nuclear Information System (INIS)

    Ream, W.K.; Espinoza, J.

    1985-01-01

    An entry control system to automatically control access to nuclear facilities is described. The design uses a centrally located console, integrated into the regular security system, to monitor the computer-controlled passage into and out of sensitive areas. Four types of entry control points are used: an unmanned enclosed portal with metal and SNM detectors for contraband detection with positive personnel identification, a bypass portal for contraband search after a contraband alarm in a regular portal also with positive personnel identification, a single door entry point with positive personnel identification, and a single door entry point with only a magnetic card-type identification. Security force action is required only as a response to an alarm. The integration of the entry control function into the security system computer is also described. The interface between the entry control system and the monitoring security personnel utilizing a color graphics display with touch screen input is emphasized. 2 refs., 7 figs

  7. Työnopastuksen kehittäminen Varuskuntaravintola Kotkassa

    OpenAIRE

    Hälikkä, Mari-Anna

    2016-01-01

    Tämän opinnäytetyön tavoitteena oli kehittää työnopastusta Varuskuntaravintola Kotkassa. Leijona Catering Oy:llä on olemassa oma perehdyttämisrunko ja materiaali uuden työntekijän yleiseen perehdyttämiseen ja koko organisaation toimintaan, mutta Varuskuntaravintola Kotkan kirjallinen materiaali toimipaikkakohtaiseen työnopastukseen oli puutteellinen ja osittain vanhentunut. Tässä työssä laadittiin toimeksiantajalle, Varuskuntaravintola Kotkalle, työnopastuksen käytäntöä tukeva työnopastusmate...

  8. Yhdessä kierrättämään : Raaseporin kaupungin työllisyysosaston työpajatoiminnan kehittämishanke

    OpenAIRE

    Westerholm, Leena

    2016-01-01

    Aktiivisen sosiaali- ja työvoimapolitiikan myötä ovat kuntien vastuut pitkäaikaistyöttömien palveluista lisääntyneet. Työllisyyskysymykset ovat muodostuneet luontevaksi osaksi sosiaalityötä. Kuntien lakisääteiset tehtävät sisältävät ikääntyvien työllistämisvelvoitteen, työmarkkinatuen rahoitusvastuun, työttömien terveyden edistämisen ja kuntouttavan työtoiminnan järjestämisen. Sosiaalihuoltolain alainen kuntouttava työtoiminta on kunnan järjestämää toimintaa, joka tähtää pitkäaikaistyöttömän ...

  9. Työskentely Norjassa : maassa työskennelleiden kokemuksia

    OpenAIRE

    Mäkäläinen, Teressa

    2013-01-01

    Tämän opinnäytetyön tarkoituksena oli antaa käytännönläheistä tietoa Norjaan muuttamisesta, työnhausta ja muista asuinmaan vaihdossa huomioon otettavista asioista, kuten verotuksesta ja sosiaaliturvasta, elinkustannuksista, asunnon hankinnasta ja käytännön elämästä. Opinnäytetyö on tarkoitettu avuksi kaikille Norjaan töihin lähteville tai sitä suunnitteleville. Tarkoituksena on tutustuttaa lukija Norjan työelämään ja maahanmuuttoa koskeviin säännöksiin, sekä auttaa Suomesta muuttamisen järjes...

  10. Työpaikkakiusaamisen synnyn syyt ravintola-alalla

    OpenAIRE

    Öström, Riikka

    2011-01-01

    Tutkimuksen taustalla on henkilökohtainen kokemus työpaikkakiusatuksi joutu-misesta ravintola-alalla. Tutkimusongelmana oli selvittää ne syyt, jotka johtavat ravintola-alalla työpaikkakiusaamiseen sekä selvittää, pätevätkö samat kiusaami-seen johtavat syyt niin ravintola-alalla kuin yleisestikin työelämässä. Tutkimuksen tarkoituksena on antaa valmiuksia työelämään sekä löytää lisää tietoa työpaikkakiusaamisesta ja kuinka sitä pystyttäisiin välttelemään. Teoriaosuudelle antaa pohjan määritelmä...

  11. Läsnätyö ja joustavat työaikamuodot -hanke Uudenmaan elinkeino-, liikenne- ja ympäristökeskuksessa

    OpenAIRE

    Karsila, Petteri

    2014-01-01

    Opinnäytetyö tarkastelee toimeksiantajayrityksen Uudenmaan elinkeino-, liikenne ja ympäristökeskuksen läsnätyö ja joustavat työaikamuodot -kokeiluhanketta. Toimeksiantajayrityksen tehtävänä on edistää alueellista kehittämistä hoitamalla valtionhallinnon toimeenpano- ja kehittämistehtäviä toimialueellaan. Tavoitteena oli selvittää läsnätyön ja mobiiliteknologian hyvät ja huonot puolet kohdeorganisaatiossa sekä arvioida, miten muuttaa kohdeorganisaation työn tekemisen tapoja, työkulttuuria...

  12. Esimiehen kehittämisvalmennuksen vaikutus hänen ja työryhmän työhyvinvointiin : Case: Osuuskauppa Arina

    OpenAIRE

    Räihä, Henna

    2017-01-01

    Tämän opinnäytetyön toimeksiantajana toimii Osuuskauppa Arina. Arinan HR-tiimin kaksi jäsentä ovat kouluttautuneet vuonna 2016 työnohjaajiksi, ja he ovat tuotteistaneet ratkaisukeskeisen työnohjauksen kehittämisvalmennukseksi. Kehittämisvalmennus on yhdistelmä ratkaisukeskeisyyttä, työnohjausta ja valmennusta, ja sen tavoitteena on saada yksilöllistä kehitystä aikaan ja valmentaa työyhteisöä ratkaisukeskeisesti. Kehittämisvalmennusta pidetään pääasiassa esimiehille. Tässä työssä tavoittee...

  13. Työkierron kehittämishanke Grand Casino Helsinki

    OpenAIRE

    Lammi, Saskia

    2010-01-01

    Opinnäytetyön aiheena on Grand Casino Helsingin (GCH) työkierron kehittämishankkeen toteuttaminen. Työkiertoa päätettiin alkaa kehittämään, koska henkilökuntaa haastateltaessa se nousi yhdeksi keinoksi lisätä kaivattua vaihtelua ja haastetta työhön. Työkierrolla tarkoitetaan sitä, että työntekijä siirtyy sovituksi ajaksi joko toiseen samantasoiseen tai eritasoiseen tehtävään ja palaa kierron lopuksi ensimmäiseen työtehtäväänsä. Työkiertoon läheisesti liittyy myös työn rikastaminen, eli om...

  14. Tuutoroinnin keinot työhön perehtymisen tukena

    OpenAIRE

    Tauriainen, Marita

    2008-01-01

    Kehittämishankkeen tavoitteena oli toteuttaa tuutorointiprosessi työpaikalla uuden työntekijän työhön perehtymisen tueksi. Kehittämishankkeessa tutkittiin tuutoroinnin erilaisia toteuttamistapoja ja merkitystä työpaikalla. Laadukkaalla tuutoroinnilla tavoitellaan uuden työntekijän nopeaa oppimista ja työpaikan tapojen sisäistämistä. Tuutoroinnin toteuttaminen on opettavaista myös tuutorille. Hän oppii uusia toteuttamismalleja ja pystyy jatkossa kehittämään tuutorin rooliaan monipuolisemmin. T...

  15. An automated entry control system for nuclear facilities

    International Nuclear Information System (INIS)

    Ream, W.K.; Espinoza, J.

    1985-01-01

    An entry control system to automatically control access to nuclear facilities is described. The design uses a centrally located console, integrated into the regular security system, to monitor the computer-controlled passage into and out of sensitive areas. Four types of entry control points are used: an unmanned enclosed portal with metal and SNM detectors for contraband detection with positive personnel identification, a bypass portal for contraband search after a contraband alarm in a regular portal also with positive personnel identification, a single door entry point with positive personnel identification, and a single door entry point with only a magnetic card-type identification. Security force action is required only as a response to an alarm. The integration of the entry control function into the security system computer is also described. The interface between the entry control system and the monitoring security personnel utilizing a color graphics display with touch screen input is emphasized

  16. Varttuneet ja ikäsyrjintä työelämässä

    OpenAIRE

    Viitasalo, Niina

    2015-01-01

    Työelämän ikäsyrjintä esteenä pitkille työurille Työelämässä esiintyvä ikäsyrjintä on ristiriidassa työurien pidentämisen kanssa. Ikäsyrjintää kokeneilla on jopa kaksinkertainen pitkien sairauspoissaolojen riski 3 vuoden seuranta-aikana. Eniten ikäsyrjintäkokemuksia on yli 55-vuotiailla naisilla, heistä lähes 8 prosenttia on kokenut ikäsyrjintää työssään. Ikäsyrjintä ilmenee työpaikkojen arjessa varttuneiden torjuntana, painostamisena, vähättelynä tai eristämisenä. Ikäsyrjintä näyttäytyy ...

  17. Työtyytyväisyys media-alan yrityksessä

    OpenAIRE

    Tolonen, Johanna; Niittyjoki, Noora

    2014-01-01

    Henkilöstön työtyytyväisyydellä on suuri vaikutus yrityksen menestymiseen ja kilpailukykyyn. Työtyytyväisyyttä pitäisi tutkia säännöllisesti, koska se ylläpitää ja edistää työntekijöiden terveyttä, hyvinvointia, motivoituneisuutta ja innovatiivisuutta. Opinnäytetyön tavoitteena oli selvittää kansainvälisen media-alan yrityksen työntekijöiden yleistä työtyytyväisyyden tilaa. Tutkimusongelmaa pyrittiin selvittämään empiirisen tutkimuksen avulla ja saamaan vastauksia kysymykseen, millä työt...

  18. Ergonomiset työtavat työelämässä : Vaivoista Voimaksi -ergonomiaopas Ravintolamaailma Sokokselle

    OpenAIRE

    Juutilainen, Jonna

    2015-01-01

    Tuki- ja liikuntaelinvaivat (tule-vaivat) ovat Suomessa toiseksi yleisin sairauspoissaoloja aiheuttava sairausryhmä. Tule-vaivoista suurin osa on niska- tai selkäperäisiä vaivoja, jotka ilmenevät työn teon aikana tai levossa. Vaivat tuovat haittaa niin yksilötasolla työntekijälle kuin myös organisaatiotasolla sekä yritykselle että valtiolle. Yksilötason haitat ovat erilaisia epämukavuustekijöitä kuten kiputiloja sekä tehokkuuteen liittyviä seikkoja kuten työkyvyn las-kua. Organisaatio- ja val...

  19. Urheiluvalmentajien työhyvinvointi Suomessa

    OpenAIRE

    Kaski, Satu

    2014-01-01

    Hyvinvoiva valmentaja koko suomalaisen urheilun etu Suomalaisessa urheilussa puhutaan muutoksesta ja pyrkimyksestä olla Pohjoismaiden paras urheilumaa vuonna 2020. Tällaisen tavoitteen edessä valmentajien työhyvinvointia ei voi sivuuttaa. Kyselytutkimukseeni vastasi 499 valmentajaa, joista suurin osa valmensi SM- tai maajoukkuetasolla olevia urheilijoita. Tutkimukseni antaa siis arvokasta tietoa suomalaisten kilpa- ja huippu-urheilussa toimivien valmentajien työhyvinvoinnista. Tutkimu...

  20. Distribution of a Ty3/gypsy-like retroelement on the A and B-chromosomes of Cestrum strigilatum Ruiz & Pav. and Cestrum intermedium Sendtn. (Solanaceae

    Directory of Open Access Journals (Sweden)

    Jéferson Nunes Fregonezi

    2007-01-01

    Full Text Available Retroelements are a diversified fraction of eukaryotic genomes, with the Ty1/copia and Ty3/gypsy groups being very common in a large number of plant genomes. We isolated an internal segment of the Ty3/gypsy retroelement of Cestrum strigilatum (Solanaceae using PCR amplification with degenerate primers for a conserved region of reverse transcriptase. The isolated segment (pCs12 was sequenced and showed similarity with Ty3/gypsy retroelements of monocotyledons and dicotyledons. This segment was used as probe in chromosomes of C. strigilatum and Cestrum intermedium. Diffuse hybridization signals were observed along the chromosomes and more accentuated terminal signals in some chromosome pairs, always associated with nucleolus organizer regions (NORs. The physical relationship between the hybridization sites of pCs12 and pTa71 ribosomal probes was assessed after sequential fluorescence in situ hybridization (FISH. Hybridization signals were also detected in the B chromosomes of these species, indicating an entail among the chromosomes of A complement and B-chromosomes.

  1. Myynnin työkalu ohjelmalliseen ostamiseen

    OpenAIRE

    Laukkonen, Patrick

    2016-01-01

    Opinnäytetyön tarkoituksena on tutustua ohjelmalliseen mediaostamiseen, sen avulla toteutettavaan huutokauppaostamiseen (real-time bidding) ja tutkia ohjelmallisen ostamisen hyötyjä perinteiseen mediaostamiseen verrattuna. Oleellisena osana opinnäytetyötä markkinointialan toimijalle on syntynyt myynnin työkalu, jota yritys pystyy käyttämään jatkossa asiakastyön tukena sekä perehdytystyökaluna omille työntekijöilleen. Myynnin työkalun sekä opinnäytetyön luomisessa on käytetty pääsääntöises...

  2. Työpisteiden tuotevirtojen ja työvälineiden hallinta : 5S-menetelmän käyttöönotto

    OpenAIRE

    Pelttari, Timo

    2014-01-01

    Työn tilaajana oli M-Components Oy Hämeenlinnasta, jonka toimialana on metallialan tuotteiden kehittely, valmistus, huolto ja kauppa. Yritys halusi ottaa käyttöön tehtaalla 5S-menetelmän, eikä siitä ollut aiempaa kokemusta yrityksessä. Työn tavoitteena oli ottaa käyttöön 5S-menetelmä ensin koneistamossa, josta se sitten laajennetaan koko yritykseen. 5S:n tavoitteena on selkeyttää työpisteiden tuotevirtoja ja työkalujen hallintaa järjestämällä työpisteet uusiksi. Kaiken tämän takana on ide...

  3. Työpaikkakouluttajan motivaatio ja motivaatioon vaikuttamisen keinot

    OpenAIRE

    Ihme, Iina-Eveliina

    2015-01-01

    Tämä opinnäytetyö tehtiin toimeksiantona Lahden Koulutuskeskus Salpaukselle. Työn päätavoitteena oli tutkia teorian ja empirian keinoin työpaikkakouluttajien motivaatiota, siihen vaikuttavia tekijöitä ja motivaatioon vaikuttamisen keinoja. Työn toisena tavoitteena oli tutkia työpaikkakouluttajien ja Koulutuskeskus Salpauksen välistä yhteydenpitoa sekä tuottaa kehitysideoita toimeksiantajan käyttöön. Opinnäytetyön tietolähteenä käytettiin aihealuetta käsittelevää lähdekirjallisuutta, kvant...

  4. The triglyceride and glucose index (TyG) is an effective biomarker to identify nonalcoholic fatty liver disease.

    Science.gov (United States)

    Zhang, Shujun; Du, Tingting; Zhang, Jianhua; Lu, Huiming; Lin, Xuan; Xie, Junhui; Yang, Yan; Yu, Xuefeng

    2017-01-19

    The triglyceride and glucose index (TyG) has been proposed as a marker of insulin resistance. We aimed to investigate the ability of TyG, through comparing with the predictive value of alanine aminotransferase (ALT), to identify individuals at risk for nonalcoholic fatty liver disease (NAFLD). A cross-sectional study was conducted in a Chinese health examination cohort of 10 761 people aged above 20 years. NAFLD was diagnosed by ultrasonography. Compared with the participants in the lowest quartile of TyG, the adjusted odds ratios (ORs) and 95% confidence intervals (CIs) for NAFLD were 1.8 (1.5-2.1), 3.0 (2.5-3.5), and 6.3 (5.3-7.5) for those in the second, the third, and the fourth quartile of TyG, whereas the corresponding ORs (95% CI) for NAFLD were 1.5 (1.3-1.7), 1.9 (1.6-2.2), and 3.1 (2.6-3.7) for the upper three quartiles of ALT. These results suggested that TyG was superior to ALT in association with NAFLD risk. According to the ROC analysis, the optimal cut-off point of TyG for NAFLD was 8.5 and the area under the ROC curve (AUC) was 0.782 (95% CI 0.773-0.790), with 72.2 and 70.5% sensitivity and specificity, respectively. The AUC of TyG was larger than that of ALT (0.715 (95% CI 0.705-0.725), P for difference <0.0001), whereas the largest AUC was obtained when adding TyG to ALT (0.804 (95% CI 0.795-0.812), P for difference <0.0001). TyG is effective to identify individuals at risk for NAFLD. A TyG threshold of 8.5 was highly sensitive for detecting NAFLD subjects and may be suitable as a diagnostic criterion for NAFLD in Chinese adults.

  5. Työvuorosuunnittelu myynnin kehittämisen työvälineenä McDonald´s-ketjussa

    OpenAIRE

    Helenius, Enna

    2015-01-01

    Tämä opinnäytetyö on McDonald´s Oy:n tilaama tutkimuksellinen kehittämistyö. Kehittämistyössä tutkittiin McDonald´s-ketjun esimiesten työvuorosuunnittelun osaamistasoa sekä työvuorosuunnittelun vaikutusta myynnin kasvattamiseen. Kehittämishankkeen tavoitteena oli luoda kehitysehdotuksia, joilla saadaan kasvatettua yksikkökohtaista myyntiä työvuorosuun-nittelun avulla. Aihetta on lähestytty tapaustutkimuksen keinoin. Opinnäytetyö koostuu tietoperustasta, ravintolapäälliköille tehdystä kyse...

  6. Työohjeistus Perälän Turve Oy

    OpenAIRE

    Haukka, Arto

    2014-01-01

    Perälän Turve Oy:n uusille työntekijöille laadittu työohjeistus ja siitä raportointi. Työhön kuuluu kaksi osaa, word-raportti sekä powerpoint-työohjeistuskansio. Powerpoint-tiedosto upotettuna tiedostona alkuperäisessä word-tiedostossa.

  7. Korikorjaamoluokituksen mukaiset työmenetelmät

    OpenAIRE

    Ylén, Niko

    2016-01-01

    Tämän opinnäytetyön tarkoituksena on selvittää auton korikorjauksessa käytettävät työmenetelmät korikorjaamoluokituksen vaatimukset huomioiden. Korikorjaamoluokituksen vaatimuksien perusteella luodaan VV-Auto Helsingin vauriokorjaamolle korikorjaustyökalujen hallinnointijärjestelmä, jonka avulla työkaluja hallinnoidaan. Lisäksi järjestelmän avulla pystytään osoittamaan korikorjaamon työkalut muun muassa korikorjaamoluokkaa auditoitaessa. Nykyaikainen auto koostuu useista erilaisista terä...

  8. Tye7 regulates yeast Ty1 retrotransposon sense and antisense transcription in response to adenylic nucleotides stress.

    Science.gov (United States)

    Servant, Géraldine; Pinson, Benoit; Tchalikian-Cosson, Aurélie; Coulpier, Fanny; Lemoine, Sophie; Pennetier, Carole; Bridier-Nahmias, Antoine; Todeschini, Anne Laure; Fayol, Hélène; Daignan-Fornier, Bertrand; Lesage, Pascale

    2012-07-01

    Transposable elements play a fundamental role in genome evolution. It is proposed that their mobility, activated under stress, induces mutations that could confer advantages to the host organism. Transcription of the Ty1 LTR-retrotransposon of Saccharomyces cerevisiae is activated in response to a severe deficiency in adenylic nucleotides. Here, we show that Ty2 and Ty3 are also stimulated under these stress conditions, revealing the simultaneous activation of three active Ty retrotransposon families. We demonstrate that Ty1 activation in response to adenylic nucleotide depletion requires the DNA-binding transcription factor Tye7. Ty1 is transcribed in both sense and antisense directions. We identify three Tye7 potential binding sites in the region of Ty1 DNA sequence where antisense transcription starts. We show that Tye7 binds to Ty1 DNA and regulates Ty1 antisense transcription. Altogether, our data suggest that, in response to adenylic nucleotide reduction, TYE7 is induced and activates Ty1 mRNA transcription, possibly by controlling Ty1 antisense transcription. We also provide the first evidence that Ty1 antisense transcription can be regulated by environmental stress conditions, pointing to a new level of control of Ty1 activity by stress, as Ty1 antisense RNAs play an important role in regulating Ty1 mobility at both the transcriptional and post-transcriptional stages.

  9. Hoitaja-lääkäri työpari : Hoitotyöntekijöiden ajatuksia ja odotuksia työparimallin toimivuudesta vastaanottotoiminnassa

    OpenAIRE

    Surakka, Iina; Pitkänen, Satu

    2009-01-01

    LAUREA-AMMATTIKORKEAKOULU TIIVISTELMÄ Laurea Hyvinkää Sosiaali-, terveys- ja liikunta-ala Hoitotyön koulutusohjelma Pitkänen Satu Surakka Iina Hoitaja-lääkäri työpari. Hoitotyöntekijöiden ajatuksia ja odotuksia työparimallin toimivuudesta vastaanottotoiminnassa Vuosi 2009 Sivu 41 Tämän opinnäytetyön tarkoituksena oli selvittää Hyvinkään vastaanottotoiminnassa työskentelevien hoitajien ajatuksia ja odotuksia liittyen hoitaja-lääkäri työparimalliin. Tutkimuste...

  10. Autoalan työssäoppiminen : Case: Autoalan merkkiliikkeet

    OpenAIRE

    Kalliomäki, Marko

    2013-01-01

    Tutkimuksen tavoitteena oli selvittää Jyväskylän ammattiopiston autoalan työssäoppimista Jyväskylän autoalan merkkiliikkeissä työnantajien näkökulmasta. Työssä selvitettiin yhteistyön nykytilanne ja millaisia ongelmia yhteistyössä koetaan olevan. Mukaan otettiin myös oppilaitoksen näkemyksiä ja kokemuksia yhteistyöstä autoalan merkkiliikkeiden kanssa työssäoppimisesta. Tutkimustulosten perusteella rakennettiin kehittämissuunnitelma yhteistyön kehittämisen pohjaksi. Tutkimusaineisto saatii...

  11. Tämä työ on kuin tehty minulle : avustajien kokemuksia työstään ja saamastaan ohjauksesta avustajatoimintahankkeessa

    OpenAIRE

    Majuri-Kiiski, Minna

    2014-01-01

    Majuri-Kiiski, Minna. Tämä työ on kuin tehty minulle ‒ Avustajien kokemuksia työstään ja saamastaan ohjauksesta avustajatoimintahankkeessa. Kevät 2014, 85 s., 5 liitettä. Diakonia-ammattikorkeakoulu, Sosiaalialan koulutusohjelma, Diakonisen sosiaalityön suuntautumisvaihtoehto, sosionomi (AMK) + diakonin virkakelpoisuus. Opinnäytetyön tarkoituksena oli selvittää Sotainvalidien Veljesliiton käynnistämässä veteraanien avustajatoimintahankkeessa työskentelevien avustajien kokemuksia omas...

  12. Työhyvinvointi on kaikkien yhteinen asia

    OpenAIRE

    Jukantupa, Anna

    2015-01-01

    Opinnäytetyön tarkoituksena oli selvittää erään päihde- ja mielenterveystyötä tekevän asumispalveluyksikön henkilöstön kokemuksia työhyvinvointiin vaikuttavista asioista ja miten näihin asioihin voi itse vaikuttaa. Tavoitteena oli lisäksi saada tietoa henkilöstön halukkuudesta osallistua työyhteisön yhteisiin työhyvinvointia ylläpitäviin hetkiin sekä kerätä tietoa, millainen tällaisen hetken sisältö voisi olla. Anonymiteetin takaamiseksi ei paljastettu yhteistyötahon nimeä. Tutkimuksen ...

  13. Effect of live Salmonella Ty21a in Dextran Sulfate Sodium-induced Colitis

    Directory of Open Access Journals (Sweden)

    Gunnar Nysœter

    2007-01-01

    Full Text Available Background Intestinal microbiota seems to play an essential role in the development of inflammatory bowel diseases (IBD. We hypothesised that an oral vaccine based on live Salmonella typhi would be well tolerated and could even attenuate dextran sulfate sodium (DSS induced colitis in rats, an animal model of IBD. Methods Nine male Wistar rats was used for an initial tolerance study, in which we used 3 dose-levels of Salmonella Ty21a, 0.5 × 10 9 , 1 × 10 9 , and 2 × 10 9 CFU, each dose being tested in 3 rats. Four treatment groups consisting of 8 male Wistar rats per group: 1 control group given standard food and water, 2 control group given four daily administrations of Salmonella Ty21a 1 × 10 9 CFU, 3 water with 5% DSS the last 7 days, 4 four daily administrations of Salmonella Ty21a before water with 5% DSS the last 7 days. The Salmonella Ty21a was administered by gastric gavage on day 1, 3, 5 and 16, while DSS was given with the drinking water from day 15 to 22. The animals were sacrificed and colonic tissue removed for analysis 22 days after gavage of the first vaccine dose. Results The animals in the tolerance study got no signs of disease. In the treatment study, all animals receiving DSS had histologic indications of colitis, particularly in the distal part of the colon. Administration of Salmonella Ty21a had no significant effect on crypt and inflammation scores (p > 0.05. Conclusion Gastric administration of live vaccine strain Salmonella Ty21a was well tolerated, but did not provide any significant protection against development of DSS induced colitis in rats.

  14. Charge conduction process and photovoltaic effects in thiazole yellow (TY) thin film based Schottky devices

    Energy Technology Data Exchange (ETDEWEB)

    Roy, M.S. [Defence Lab., Jodhpur (India). Camouflage Div.; Sharma, G.D.; Gupta, S.K. [Department of Physics, J.N.V. University, Jodhpur (Raj.) (India)

    1997-11-21

    The charge generation and photovoltaic effects observed with thin films of TY in the form of sandwich structures, were analysed by J-V, C-V and photoaction spectra. These measurements were explained in terms of n-type semiconductivity of TY thin film and by the formation of a Schottky barrier with ITO while Ohmic contact with an Al or In electrode. The existence of thermionic emission over the ITO-TY barrier has been observed in low voltage region, whereas at high voltages, the process is dominant by the series resistance of TY layer. Various electrical parameters were calculated from the analysis of J-V and C-V characteristics of the devices and discussed in details. The diode quality factor is higher for Al/TY/ITO than In/TY/ITO device which can be attributed to the formation of thin layer of Al{sub 2}O{sub 3} between Al and TY. The photoaction spectra of the devices reveal that the fraction of light which is absorbed near the ITO-TY interface, to the depth of 180 A, is responsible for producing the charge carriers. The photovoltaic parameters were also calculated from the J-V characteristics of the devices, under illumination and described in detail. (orig.) 21 refs.

  15. Perehdytys etätyössä : case Annanpura Oy:n perehdytyssuunnitelma

    OpenAIRE

    Kohomäki, Noora

    2013-01-01

    Opinnäytetyön tavoitteena oli laatia toimiva perehdytyssuunnitelma yritykselle, jonka työntekijät työskentelevät pääsääntöisesti kotoaan käsin. Etätyön perehdyttämisessä korostuvat riittävän viestinnän ja vuorovaikutuksen järjestäminen. Työn keskeisenä haasteena oli dialogisen perehdytyksen ja itsenäisesti suoritettavan etätyön yhdistäminen. Työn tavoitteena oli lisäksi selvittää, onnistuuko hiljaisen tiedon ja osaamisen siirtäminen etätyössä ja sen perehdytyksessä. Teoreettisessa viitekeh...

  16. Regulators of ribonucleotide reductase inhibit Ty1 mobility in saccharomyces cerevisiae

    Directory of Open Access Journals (Sweden)

    O'Donnell John P

    2010-11-01

    Full Text Available Abstract Background Ty1 is a long terminal repeat retrotransposon of Saccharomyces cerevisiae, with a replication cycle similar to retrovirus replication. Structurally, Ty1 contains long terminal repeat (LTR regions flanking the gag and pol genes that encode for the proteins that enable Ty1 mobility. Reverse transcriptase produces Ty1 complementary (cDNA that can either be integrated back into the genome by integrase or recombined into the yeast genome through homologous recombination. The frequency of Ty1 mobility is temperature sensitive, with optimum activity occurring at 24-26°C. Results In this study, we identified two host genes that when deleted allow for high temperature Ty1 mobility: RFX1 and SML1. The protein products of these genes are both negative regulators of the enzyme ribonucleotide reductase, a key enzyme in regulating deoxyribonucleotide triphosphate (dNTP levels in the cell. Processing of Ty1 proteins is defective at high temperature, and processing is not improved in either rfx1 or sml1 deletion strains. Ty1 mobility at high temperature is mediated by homologous recombination of Ty1 cDNA to Ty1 elements within the yeast genome. We quantified cDNA levels in wild type, rfx1 and sml1 deletion background strains at different temperatures. Southern blot analysis demonstrated that cDNA levels were not markedly different between the wild type and mutant strains as temperatures increased, indicating that the increased Ty1 mobility is not a result of increased cDNA synthesis in the mutant strains. Homologous recombination efficiency was increased in both rfx1 and sml1 deletion strains at high temperatures; the rfx1 deletion strain also had heightened homologous recombination efficiency at permissive temperatures. In the presence of the dNTP reducing agent hydroxyurea at permissive temperatures, Ty1 mobility was stimulated in the wild type and sml1 deletion strains but not in the rfx1 deletion strain. Mobility frequency was greatly

  17. Osaamisen kehittämisen haasteet, esteet ja kannusteet työuran keskivaiheen muutostilanteissa

    OpenAIRE

    Törmälä-Rantala, Päivi

    2017-01-01

    Työn tavoitteena oli tutkia osaamisen kehittämisen haasteita, esteitä ja kannusteita työuran puolivälissä. Tutkimuksessa selvitettiin, miksi toinen opiskelee ja koulutuksen avulla muuttaa työtilannettaan aktiivisesti, kun taas toinen jättää osaamisensa kehittämisen ja jämähtää samaan työtehtävään vuosiksi. Kartoitus toimi taustatietona Työterveyslaitoksen Taidot työhön –hankkeeseen. Hanke toteutettiin interventiona, ja siinä opastettiin ja kannustettiin ihmisiä parantamaan työllistymiskykyään...

  18. Työkierto : Mielipiteitä käytännöstä

    OpenAIRE

    Hietala, Teija; Lehtonen, Anna

    2012-01-01

    Tämän opinnäytetyön tarkoituksena oli selvittää hoitajien kokemuksia työkierrosta eräillä yliopistollisen sairaalan osastoilla. Tavoitteena oli antaa tietoa osastojen henkilökunnalle työkierron toimivuudesta. Tavoitteena oli, että kyselyn tuloksia voitaisiin käyttää apuna työkierron toimivuuden arvioinnissa ja mahdollisessa kehittämisessä. Opinnäytetyön tehtävinä oli selvittää mitä työkierto on, miten osaston työntekijät kokivat työkierron toimivuuden osastoilla ja miten osastojen työntekijät...

  19. Hanford Single-Shell Tank Leak Causes and Locations - 241-BY and 241-TY Farm

    Energy Technology Data Exchange (ETDEWEB)

    Girardot, Crystal L.; Harlow, Donald G.

    2014-09-04

    This document identifies 241-BY Tank Farm (BY Farm) and 241-TY Tank Farm (TY Farm) lead causes and locations for the 100 series leaking tanks (241-BY-103, 241-TY-103, 241-TY-104, 241-TY-105 and 241-TY-106) identified in RPP-RPT-43704, Hanford BY Farm Leak Assessments Report, and in RPP-RPT-42296, Hanford TY Farm Leak Assessments Report. This document satisfies the BY and TY Farm portion of the target (T04) in the Hanford Federal Facility Agreement and Consent Order milestone M-045-91F.

  20. Hoitohenkilökunnnan näkemyksiä tavoitteellisesta työkierrosta

    OpenAIRE

    Neuvonen, Elina

    2015-01-01

    Opinnäytetyöni tarkoituksena oli kuvailla tavoitteelliseen työkiertoon osallistumattoman hoitohenkilökunnan näkemyksiä tavoitteellisesta työkierrosta. Hoitohenkilökunnalla tässä työssä tarkoitetaan perus-, lähi-, ja mielenterveys- ja sairaanhoitajia. Tavoitteena oli kuvailla hoitohenkilökunnan näkemyksiä tavoitteellisesta työkierrosta, kartoittaa mitä tietoa heillä on sekä selvittää, mitkä tekijät edistäisivät hoitohenkilökunnan työkiertoon lähtemistä ja toisaalta mitkä tekijät sitä estävät. ...

  1. Venäjä-opas IKEA Kuopion työntekijöille

    OpenAIRE

    Vartiainen, Noora

    2013-01-01

    Tämän opinnäytetyön aiheena oli luoda IKEA Kuopion työntekijöille venäjä-opas venäläisten asi-akkaiden palvelua varten. Työn tarkoituksena oli auttaa Kuopion IKEA – tavaratalon työntekijöitä kommunikoimaan venäläisten asiakkaiden kanssa ja palvelemaan heitä paremmin. Työn tavoitteena oli tehdä opas, joka sisältää käytännöllisiä ja oleellisia lauseita venäjäksi IKEA Kuopion työntekijöiden työn kannalta sekä on helppokäyttöinen. Opas sisältää tietoa venäläisistä, jotta työntekijät ymmärtävät en...

  2. Kehityskeskustelut osana työyhteisön johtamista

    OpenAIRE

    Nikula, Johannes

    2015-01-01

    Tämän päivän suomalaisessa työelämässä eletään jatkuvassa muutoksessa, joka on seurausta kiristyneestä kansainvälisestä kilpailusta. Menestyäkseen alati kovenevassa kilpailussa organisaatioiden on saatava jokaisen työntekijän potentiaali käyttöönsä täysimääräisesti. Kehityskeskustelut tarjoavat organisaatioiden johdolle, esimiehille ja työntekijöille viestintäkanavan käydä kehittävää vuoropuhelua yhteisen suunnan löytämiseksi. Kehityskeskustelujen avulla organisaatioissa johdetaan suoritusta,...

  3. Rakennusalan työsaliopetuksen arviointi Omnian ammattiopistossa

    OpenAIRE

    Huttunen, Ari Petteri

    2010-01-01

    TIIVISTELMÄ Taustaa Espoon seudun koulutuskuntayhtymä on pääkaupunkiseudun aktiivisempia ja isoimpia rakennusalan kouluttajia. Omnian ammattiopisto antaa rakennusalan perustutkintoon johtavaa koulutusta. Koulutusohjelma on talonrakennus. Omnian muuttaessa uusiin tiloihin kolme vuotta sitten, havaittiin että rakennusalan työsalin opetusta tulisi kehittää. Tavoite Tavoitteena oli että saisimme kehittää nuorten rakennusalan koulutusta työsalilla tapahtuvassa II-asteen koulutuksen o...

  4. Sosiaalisen median markkinointisuunnitelma uudelle hoitoalan konseptoidulle työvaatemallistolle

    OpenAIRE

    Leppälä, Sanna

    2017-01-01

    Opinnäytetyössä pohdittiin keinoja saada uuden konseptoidun työvaatemalliston näkyvyyttä esille sosiaalisen median keinoin. Toimeksiantaja-yrityksenä toimi suomalainen Virtually Oy ja kohteena oli sen uusi hoitajille suunnattu työvaatemallisto. Malliston ympärille halutaan luoda kestävä brändi ja sosiaalisen median markkinointi on tukemassa brändin tunnettavuutta ja myyntiä. Sosiaalinen media ei yritysten välisessä liiketoiminnassa toimi ainoana markkinoinnin työkaluna, vaan se on tukemas...

  5. The TyG index may predict the development of cardiovascular events.

    Science.gov (United States)

    Sánchez-Íñigo, Laura; Navarro-González, David; Fernández-Montero, Alejandro; Pastrana-Delgado, Juan; Martínez, Jose Alfredo

    2016-02-01

    Cardiovascular disease (CVD) is the worldwide leading cause of morbidity and mortality. An early risk detection of apparently healthy people before CVD onset has clinical relevance in the prevention of cardiovascular events. We evaluated the association between the product of fasting plasma glucose and triglycerides (TyG index) and CVD. A total of 5014 patients of the Vascular Metabolic CUN cohort (VMCUN cohort) were followed up during a median period of 10 years. We used a Cox proportional-hazard ratio with repeated measures to estimate the risk of incidence of CVD across quintiles of the TyG index, calculated as ln[fasting triglycerides (mg/dL) × fasting plasma glucose (mg(dL)/2], and plotted a receiver-operating characteristics (ROC) curve to compare a prediction model fitted on the variables used in the Framingham risk score, a new model containing the Framingham variables with the TyG index, and the risk of coronary heart disease. A higher level of TyG index was significantly associated with an increased risk of developing CVD independent of confounding factors with a value of 2·32 (95% CI: 1·65-3·26) for those in the highest quintile and 1·52 (95% CI: 1·07-2·16) for those in the fourth quintile. The areas under the curve (AUC) of the ROC plots were 0·708 (0·68-0·73) for the Framingham model and 0·719 (0·70-0·74) for the Framingham + TyG index model (P = 0·014). The TyG index, a simple measure reflecting insulin resistance, might be useful to early identify individuals at a high risk of developing a cardiovascular event. © 2015 Stichting European Society for Clinical Investigation Journal Foundation.

  6. Asiakaspalveluosaston työtyytyväisyys ja sitoutuminen

    OpenAIRE

    Puustinen, Piia

    2015-01-01

    Opinnäytetyön tavoitteena oli mitata toimeksiantajayrityksen X asiakaspalveluosaston työviihtyvyyden sekä työhön sitoutumisen tasoa selvittämällä näihin eniten vaikuttavat tekijät sekä näiden tekijöiden toteutumisen taso henkilökunnan näkökulmasta. Opinnäytetyön toimeksiantajana ollut kansainvälinen logistiikka-alan yritys halusi pysyä nimettömänä työn aiheen vuoksi, joten tästä syystä opinnäytetyössä käytettiin nimeä Yritys X. Sitouttaminen voidaan määritellä henkilöstöjohtamisen keinoks...

  7. Hyvät kokemukset luovat lumipalloilmiön. : kartoitus näkövammaisten henkilöiden työllistymisestä työkeskuksiin ja sosiaalisiin yrityksiin.

    OpenAIRE

    Vehviläinen, Timo

    2013-01-01

    TIIVISTELMÄ Vehviläinen, Timo. Hyvät kokemukset luovat lumipalloilmiön. Näkövammaisten henkilöiden työllistyminen työkeskuksiin ja sosiaalisiin yrityksiin. Diak Helsinki, syksy 2013, 58 s., 2 liitettä. Diakonia-ammattikorkeakoulu, Helsingin toimipaikka, Sosiaalialan koulutusohjelma, sosio-nomi (AMK). Tämän tutkimuksellisen opinnäytetyön aiheena oli näkövammaisten henkilöiden työllisty-minen. Siinä kartoitettiin työllistymiseen vaadittavia keskeisiä tukitoimia ja apuvälineitä sekä ...

  8. Facebook lanseerauksen työkaluna : Case: Flypark

    OpenAIRE

    Rautio, Hanna

    2015-01-01

    Opinnäytetyön tarkoituksena on tuoda esille nykyajan tuomaa muutosta markkinointiin ja sosiaalisen median mahdollisuuksia mainonnan välineenä. Työssä tutustutaan viime vuosina tapahtuneeseen markkinoinnin kehitykseen ja rahallisten panostusten muutoksiin Suomessa. Esimerkkinä työssä toimii viime syksynä toimintansa aloittanut pysäköintiyritys Flypark, joka toteutti lanseerauskampanjan Facebookissa tavoitteena kasvattaa tunnettuutta, sekä kerätä potentiaalisia asiakkaita sosiaalisen median avu...

  9. Perhetyöntekijöiden työnkuva ja ajankäyttö

    OpenAIRE

    Rantala, Miia

    2017-01-01

    Opinnäytetyön tarkoituksena oli tarkastella Porin perusturvakeskuksen perhetyöntekijöiden työnkuvaa. Tutkimuksessa selvitettiin, minkälaisia työtehtäviä perhetyöntekijän työhön kuuluu ja kuinka paljon yksittäiset työtehtävät vievät työaikaa. Opinnäytetyön teoreettisessa osuudessa käsiteltiin yleisesti perhetyötä ja mitä se tarkoittaa erilaisista näkökulmista. Perhetyöstä kerrottiin niin ennaltaehkäisevästä kuin lastensuojelunkin näkökulmasta, myös lain säädäntöä ja työvälineitä sivuttiin...

  10. Strain improvement of Pichia kudriavzevii TY13 for raised phytase production and reduced phosphate repression.

    Science.gov (United States)

    Qvirist, Linnea; Vorontsov, Egor; Veide Vilg, Jenny; Andlid, Thomas

    2017-03-01

    In this work, we present the development and characterization of a strain of Pichia kudriavzevii (TY1322), with highly improved phytate-degrading capacity. The mutant strain TY1322 shows a biomass-specific phytate degradation of 1.26 mmol g -1  h -1 after 8 h of cultivation in a high-phosphate medium, which is about 8 times higher compared with the wild-type strain. Strain TY1322 was able to grow at low pH (pH 2), at high temperature (46°C) and in the presence of ox bile (2% w/v), indicating this strain's ability to survive passage through the gastrointestinal tract. The purified phytase showed two pH optima, at pH 3.5 and 5.5, and one temperature optimum at 55°C. The lower pH optimum of 3.5 matches the reported pH of the pig stomach, meaning that TY1322 and/or its phytase is highly suitable for use in feed production. Furthermore, P. kudriavzevii TY1322 tolerates ethanol up to 6% (v/v) and shows high osmotic stress tolerance. Owing to the phenotypic characteristics and non-genetically modified organisms nature of TY1322, this strain show great potential for future uses in (i) cereal fermentations for increased mineral bioavailability, and (ii) feed production to increase the phosphate bioavailability for monogastric animals to reduce the need for artificial phosphate fortification. © 2016 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology.

  11. Entry into force of the Convention on Supplementary Compensation for Nuclear Damage: Opening the umbrella

    International Nuclear Information System (INIS)

    McRae, Ben

    2015-01-01

    There are 431 commercial nuclear power plants around the world. On 14 April 2015, 193 of these power plants were covered by a nuclear liability instrument (118 power plants by the Paris Convention and 75 by the Vienna Convention). With the entry into force of the Convention on Supplementary Compensation for Nuclear Damage (CSC)4 on 15 April 2015, the number of power plants covered by a nuclear liability instrument increased to 340. Thus, the entry into force of the CSC marked a major milestone towards the establishment of a global nuclear liability regime. This article discusses several events that have promoted progress towards a global nuclear liability regime and then addresses several questions that may arise as countries consider actions necessary to achieve such a regime. (author)

  12. Työaikaergonomia eräässä hoiva-alan yrityksessä

    OpenAIRE

    Airaksinen, Riikka

    2012-01-01

    Vuorotyön haitallisista vaikutuksista ihmisen terveyteen ja hyvinvointiin on laajaa tutkimusnäyttöä, mutta terveydenhuoltoalalla epäsäännöllinen vuorotyö on edelleen erittäin yleistä. Vuorotyön haitallisia vaikutuksia on mahdollista vähentää huomioimalla työaikaergonomia työvuorosuunnittelussa. Tämän tapaustutkimuksen tarkoituksena oli kartoittaa erään hoiva-alan yrityksen hoitohenkilöstön työaikaergonomiaa, siihen vaikuttavia tekijöitä sekä hoitohenkilöstön mielipiteitä työaikaergonomias...

  13. IUE observations of W UMa systems AE Phoenicis and TY Mensae

    International Nuclear Information System (INIS)

    Rucinski, S.M.

    1984-01-01

    SWP spectra of AE Phe and TY Men are presented and discussed. The spectrum of AE Phe is typical in showing strong emission lines which originate in the chromosphere and transition region. On the basis of the strenght of the He II emission line we predict that AE Phe should be a moderately strong X-ray source. The spectrum of TY Men is too weakly exposed for a full discussion. It is pointed out that this star is one of the most important for understanding the activity in W UMa systems. 18 refs., 3 figs., 1 tab. (author)

  14. Port entry of nuclear ships differences, procedures and conditions

    International Nuclear Information System (INIS)

    McMichael, D.; Bianchi, H.

    1978-01-01

    There are about 250 nuclear propelled ships, only 2 of them are merchant ships: the US freighter Savannah and the bulk carrier Otto Hahn of the Federal Republic of Germany. This paper is restricted to the experience with both of these ships, showing partly similar and partly different procedures for port entry. They have called at 107 ports in 40 countries. The Savannah was operated in the periods from 1962 - 1971 and the Otto Hahn is still in operation. The endeavours of the OECD and IMCO to review and to harmonize international rules for nuclear merchant ships should be supported and continued

  15. Röntgenhoitajaopiskelijan työturvallisuus ammattitaitoa edistävässä harjoittelussa

    OpenAIRE

    Vierula, Hanna-Leena

    2011-01-01

    Opinnäytetyönä tuotettiin taskuinfo työturvallisuudesta Metropolia Ammattikorkeakoulun radiografian ja sädehoidon koulutusohjelman opiskelijoille ammattitaitoa edistäviin harjoitteluihin. Opinnäytetyön tavoite on edistää röntgenhoitajaopiskelijoiden tietoutta työturvallisuudesta sekä auttaa heitä työturvallisuuteen liittyvissä ongelmatilanteissa ammattitaitoa edistävissä harjoitteluissa. Opinnäytetyössä tuotettiin materiaalia opiskelijoiden työturvallisuuteen liittyen. Pohjana käytettiin...

  16. PUVUSTUS REUNAN YLI – NUORISONÄYTELMÄÄN : Pukusuunnittelija osana työryhmää

    OpenAIRE

    Savinoff, Kirsi

    2011-01-01

    Tarkastelen tässä opinnäytetyössä pukusuunnittelijan työnkuvaa ja roolia näytelmäproduktion yhtenä rakentajana. Työssäni pohdiskelen sitä kuinka pukusuunnittelija työskentelee osana ammattimaista teatteriryhmää ja, kuinka ryhmän sisäinen tiimityöskentely vaikuttaa työn kulkuun ja lopullisen työn laatuun. Työni rajaan käsittelemään pukusuunnittelijan kannalta neljää tärkeintä produktioissa mukana olevaa tahoa ohjaajaa, näyttelijää, lavastajaa ja valmistajia eli ompelimjoita. Tietoutta pukusuun...

  17. Structure-Function Model for Kissing Loop Interactions That Initiate Dimerization of Ty1 RNA

    Directory of Open Access Journals (Sweden)

    Eric R. Gamache

    2017-04-01

    Full Text Available The genomic RNA of the retrotransposon Ty1 is packaged as a dimer into virus-like particles. The 5′ terminus of Ty1 RNA harbors cis-acting sequences required for translation initiation, packaging and initiation of reverse transcription (TIPIRT. To identify RNA motifs involved in dimerization and packaging, a structural model of the TIPIRT domain in vitro was developed from single-nucleotide resolution RNA structural data. In general agreement with previous models, the first 326 nucleotides of Ty1 RNA form a pseudoknot with a 7-bp stem (S1, a 1-nucleotide interhelical loop and an 8-bp stem (S2 that delineate two long, structured loops. Nucleotide substitutions that disrupt either pseudoknot stem greatly reduced helper-Ty1-mediated retrotransposition of a mini-Ty1, but only mutations in S2 destabilized mini-Ty1 RNA in cis and helper-Ty1 RNA in trans. Nested in different loops of the pseudoknot are two hairpins with complementary 7-nucleotide motifs at their apices. Nucleotide substitutions in either motif also reduced retrotransposition and destabilized mini- and helper-Ty1 RNA. Compensatory mutations that restore base-pairing in the S2 stem or between the hairpins rescued retrotransposition and RNA stability in cis and trans. These data inform a model whereby a Ty1 RNA kissing complex with two intermolecular kissing-loop interactions initiates dimerization and packaging.

  18. Onko Facebookista hyötyä pienyritysten markkinoinnissa?

    OpenAIRE

    Huida, Niina

    2012-01-01

    Sosiaalisesta mediasta on tullut yhä merkittävämpi osa yritysten markkinointiviestintää. Opinnäytetyön tavoitteena on kehittää sosiaalisen median käyttöä pienyritysten markkinoinnissa ja tarkoituksena on selvittää pienyritysten saama hyöty markkinoimisesta sosiaalisessa mediassa. Tutkimuksessa keskityn verkkoyhteisö Facebookin yrityksille tarjoamiin markkinointimahdollisuuksiin. Opinnäytetyön tehtävänä on tutkimuksen avulla vastata ongelmaan ”Onko Facebookista hyötyä pienten yritysten markkin...

  19. A study on surveillance equipment at the exit/entry control point of nuclear facility

    International Nuclear Information System (INIS)

    Park, C. S.; Kim, D. Y.; Cha, H. L.; Kim, H. D.; Hong, J. S.

    1999-01-01

    Exit/Entry control is an essential measure at both entrances of the protected area and vital area of nuclear facility at which physical protection is required under the relevant laws and regulations. Especially, when there are heavy traffics of personnel and process equipment in those areas, automated surveillance devices have to be introduced to timely and efficiently screen out internal and external adversaries from achieving their goals of stealing of nuclear material and/or sabotage of the facility. The major portion of this study involves with integration and processing of signals from radiation detector, metal detector, and image monitor. This integrated device together with positive personal identification device which will be reinforced in near future would contribute to the establishment of total exit/entry control point of nuclear facility

  20. Työelämäosaaminen: kvalifikaatioiden luokitusjärjestelmän konstruointi

    OpenAIRE

    Hanhinen, Taina

    2010-01-01

    YTM Taina Hanhinen käsittelee ammattikasvatustieteen väitöstutkimuksessaan työelämäosaamisen ilmiötä eri näkökulmista ja eri tasoilla. Tutkimuksen tuloksena esitetään työelämäosaamisen teoreettinen malli. Se kuvaa jatkuvassa muutoksessa olevaa työelämän kenttää, jossa työorganisaatiot ja niiden jäsenet aktiivisesti kehittävät omaa osaamistaan, toimintaansa sekä toimintaympäristöään. Tutkimuksessa työelämäosaamisen keskeisiksi osatekijöiksi jäsentyivät kvalifikaatiot ja kompetenssi sekä am...

  1. RCRA Assessment Plan for Single-Shell Tank Waste Management Area TX-TY

    Energy Technology Data Exchange (ETDEWEB)

    Horton, Duane G.

    2007-03-26

    WMA TX-TY contains underground, single-shell tanks that were used to store liquid waste that contained chemicals and radionuclides. Most of the liquid has been removed, and the remaining waste is regulated under the RCRA as modi¬fied in 40 CFR Part 265, Subpart F and Washington State’s Hazardous Waste Management Act . WMA TX-TY was placed in assessment monitoring in 1993 because of elevated specific conductance. A groundwater quality assessment plan was written in 1993 describing the monitoring activities to be used in deciding whether WMA TX-TY had affected groundwater. That plan was updated in 2001 for continued RCRA groundwater quality assessment as required by 40 CFR 265.93 (d)(7). This document further updates the assessment plan for WMA TX-TY by including (1) information obtained from ten new wells installed at the WMA after 1999 and (2) information from routine quarterly groundwater monitoring during the last five years. Also, this plan describes activities for continuing the groundwater assessment at WMA TX TY.

  2. Anti-sense expression of a metallopeptidase gene enhances nuclear entry of HBV-DNA

    International Nuclear Information System (INIS)

    Yeh, C.-T.; Lai, H.-Y.; Chu, S.-P.; Tseng, I-Chu

    2004-01-01

    Although several putative hepatitis B virus (HBV) receptors have been identified, none of them is capable of initiating HBV replication in a non-permissive human cell line. Using an Epstein-Barr virus-based extrachromosomal replication system, we have screened through a human liver cDNA library and successfully identified a clone capable of facilitating nuclear transport of HBV-DNA during the early phase of HBV infection. This clone contained a cDNA encoding a metallopeptidase-like protein in anti-sense orientation. Pretreatment of naive HepG2 cells with 1,10-phenanthroline, an inhibitor for liver metallopeptidases, led to nuclear entry of HBV-DNA after HBV infection. However, cccDNA was still undetectable in the nuclei, indicating other cellular factors required to complete the replication cycle were still missing. Our present data suggest that in the initial stage of HBV infection, liver metallopeptidase constitutes a barrier for effective nuclear entry of HBV genomic DNA. Attenuation of metallopeptidase activity may facilitate HBV infection

  3. Linjasaneerauksen työvaiheiden yhteensovituksen vaikutus linja-aikatauluun

    OpenAIRE

    Luomala, Joni

    2017-01-01

    Opinnäytetyössä käsitellään perinteistä putkiremonttia, sen aikataulua ja aikataulun yhteensovittamisen vaikutusta lopulliseen linja-aikatauluun. Opinnäytetyö tehtiin pääkaupunkiseudulla toimivalle talotekniikkaurakointiyritykselle LVIS-Hokka Oy:lle. Opinnäytetyössä perehdytään tarkemmin 1960–70-luvulla rakennettujen kerrostalojen perinteiseen putkiremonttiin ja siinä tehtäviin työvaiheisiin. Työssä on esitelty LVI-, sähkö ja rakennustöiden laajuus huoneistoittain yhden LVIS-Hokka Oy:n li...

  4. Tuotekoulutuksen vaikutus kosmetiikkamyyjän työmotivaatioon

    OpenAIRE

    Leppäkorpi, Elina

    2017-01-01

    Tämän opinnäytetyön tarkoituksena oli tutkia tuotekoulutuksen vaikutusta kosmetiikkamyyjän työmotivaatioon. Opinnäytetyön yhteistyökumppanina toimi Estée Lauder Finland Oy. Työn teoreettinen viitekehys käsittelee myyntityötä, motivaatiota sekä koulutusta. Myyntityön osuudessa keskitytään myynnin suunnitteluun ja myyntiprosessiin. Motivaation osuus käsittelee motivaatiota käsitteenä sekä siihen vaikuttavia tekijöitä. Koulutuksen osuudessa käsitellään koulutusprosessia ja koulutuksen menetelmiä...

  5. Mobile Officen sisustussuunnitelma : Rytmikorjaamon liikkuvan ihmisen työpisteet

    OpenAIRE

    Talvitie, Satu

    2011-01-01

    Opinnäytetyö käsitteli toimistotilan sisustussuunnittelua luovan tilan konseptilla. Työn tarkoituksena oli antaa selkeä kuva sisustussuunnittelusta, toimistosuunnittelusta ja luovan tilan merkityksestä. Opinnäytetyön tavoitteena oli tehdä sisustussuunnitelma Rytmikorjaamon Mobile Officeen. Mobile Office tehdään liikkuvien ihmisten työpisteiksi. Opinnäytetyön toimeksiantajana toimivat Sisustuskeskus Sella, Seinäjoen kaupunki, Selmu Ry ja Seinäjoen teknologiakeskus. Opinnäytetyön teoriaosuud...

  6. Statement to the Third Conference on Facilitating the Entry into Force of the Comprehensive Nuclear-Test-Ban Treaty (CTBT). Vienna, 3 September 2003

    International Nuclear Information System (INIS)

    ElBaradei, M.

    2003-01-01

    Full text: The Third Conference on Facilitating the Entry into Force of the Comprehensive Nuclear-Test-Ban Treaty (CTBT) is being held at a time of major challenges to the nuclear arms control and disarmament regime. A verified, permanent, global ban on all types of nuclear explosive tests has been a key item on the international security agenda for nearly half a century. More than 2,000 nuclear explosive detonations have taken place since 1945, with the most recent ones in 1998. The CTBT has been characterized as the longest sought, hardest fought prize in the history of arms control. The Treaty, when implemented, will prohibit all nuclear explosions, in all environments, for all time. It will curb the development and testing of new, more advanced and more dangerous nuclear weapons, and will limit the possibilities for further nuclear proliferation. The Treaty will lead to the establishment of a comprehensive International Monitoring System to provide independent, impartial verification of compliance. The CTBT, along with the Treaty on the Non-Proliferation of Nuclear Weapons (NPT), and a future Treaty Banning the Production of Fissile Material for Nuclear Weapons and Other Nuclear Explosive Devices (FMCT), forms an essential element of a network of negotiated, global treaties that will strengthen international efforts to prevent the further spread of nuclear weapons and to promote nuclear disarmament leading in time hopefully to a world without nuclear weapons. In the meantime, with the early entry into force of the CTBT, it would indeed be a significant achievement if this new century were to remain free of any nuclear test explosions. In this context, I encourage all signatory States to ratify the CTBT, and all those States that have yet to sign to do so and to ratify the Treaty, as soon as possible - so that another crucial pillar can be raised to support the edifice of global nuclear non-proliferation and nuclear disarmament norms. (IAEA)

  7. Jaksetaan, jookos? : Päiväkodin johtajien kokemuksia psykososiaalisesta työhyvinvoinnista

    OpenAIRE

    Puranen, Tiina

    2011-01-01

    Opinnäytetyön tarkoituksena on kuvata päiväkodin johtajien psykososiaalista työhyvinvointia: kuin-ka päiväkodin johtajat jaksavat työssään ja työyhteisössä. Kysely suunnattiin Kuopion kaupungin, Siiliset- peruspalvelukeskuksen, Suonenjoen kaupungin, Rautalammin kunnan sekä muutamien yksityisten päiväkotien johtajille (N=56). Vastausprosentti oli 59 % (n=33). Tutkimusaineisto kerättiin kyselylomakkeella maalis-huhtikuun aikana vuonna 2011. Kyselylomakkeessa käytettiin pääosin yleistä pohjoi...

  8. Two large-scale analyses of Ty1 LTR-retrotransposon de novo insertion events indicate that Ty1 targets nucleosomal DNA near the H2A/H2B interface

    Directory of Open Access Journals (Sweden)

    Bridier-Nahmias Antoine

    2012-12-01

    Full Text Available Abstract Background Over the years, a number of reports have revealed that Ty1 integration occurs in a 1-kb window upstream of Pol III-transcribed genes with an approximate 80-bp periodicity between each integration hotspot and that this targeting requires active Pol III transcription at the site of integration. However, the molecular bases of Ty1 targeting are still not understood. Findings The publications by Baller et al. and Mularoni et al. in the April issue of Genome Res. report the first high-throughput sequencing analysis of Ty1 de novo insertion events. Their observations converge to the same conclusion, that Ty1 targets a specific surface of the nucleosome at he H2A/H2B interface. Conclusion This discovery is important, and should help identifying factor(s involved in Ty1 targeting. Recent data on transposable elements and retroviruses integration site choice obtained by large-scale analyses indicate that transcription and chromatin structure play an important role in this process. The studies reported in this commentary add a new evidence of the importance of chromatin in integration selectivity that should be of interest for everyone interested in transposable elements integration.

  9. Ammatillisesti syvennetyn lääketieteellisen kuntoutuksen vaikuttavuus. Kuntoutus osana työpaikan terveyden edistämistä

    OpenAIRE

    Turja, Johanna

    2009-01-01

    Väitöskirjatutkimuksessa selvitettiin kontrolloidulla seurantatutkimuksella ammatillisesti syvennetyn lääketieteellisen kuntoutuksen (ASLAK®) jatkumista työpaikalla, sen pitkäaikaista vaikutusta kuntoutujien henkiseen hyvinvointiin, koettuun terveyteen ja työkykyyn sekä työpaikan fyysisiin ja psykososiaalisiin työolosuhteisiin. Tutkimuksen mukaan kuntoutuksella ei ollut näihin asioihin pitkäaikaista vaikutusta. ASLAK®-kuntoutus on varhaiskuntoutuksen muoto, jolla pyritään vaikuttamaan työ...

  10. Työnantajien mielikuvia ammattiryhmästä - vertailussa sihteerit, HSO-sihteerit ja tradenomi, HSOt

    OpenAIRE

    Kohtala, Hannele

    2010-01-01

    Tutkimuksen tavoitteena oli selvittää, millaisia mielikuvia suomalaisilla työnantajilla on sihteerien ja assistenttien ammattiryhmästä. Opinnäytetyössä tutkittiin työnantajien tietämystä erilaisista sihteerien nimikkeistä, kuten HSO-sihteeristä ja tradenomi, HSOsta. Haluttiin tietää, millä sektorilla työskentelevät henkilöt tuntevat eri nimikkeet parhaiten. Samalla selvitettiin, onko naistyönantajilla parempi tietämys kuin miehillä ja minkä ikäiset työnantajat osaavat parhaiten erottaa nimikk...

  11. Turvajalkineiden vaikutus jalkaterveyteen Suur-Savon Sähkötyö Oy:ssä

    OpenAIRE

    Pulkkinen, Riikka; Tossavainen, Inka; Ryynänen, Elina

    2014-01-01

    Opinnäytetyön tarkoituksena oli selvittää, millaisia vaikutuksia turvajalkineilla voi olla jalkaterveyteen, minkälaisia jalkaongelmia turvajalkineiden käyttö voi aiheuttaa, voivatko työntekijät itse vaikuttaa turvajalkineiden hankintaan ja millainen on turvajalkineiden hankintaprosessi Suur-Savon Sähkötyö Oy:ssä. Opinnäytetyömme toimeksiantaja oli Suur-Savon Sähkötyö Oy. Tutkimusmenetelmänä käytettiin pääasiassa kvantitatiivista tutkimusta ja aineisto kerättiin puolistrukturoidulla kysely...

  12. Phosphoinositide-3-kinases p110alpha and p110beta mediate S phase entry in astroglial cells in the marginal zone of rat neocortex

    Directory of Open Access Journals (Sweden)

    Rabea eMüller

    2013-03-01

    Full Text Available In cells cultured from neocortex of newborn rats, phosphoinositide-3-kinases of class I regulate the DNA synthesis in a subgroup of astroglial cells. We have studied the location of these cells as well as the kinase isoforms which facilitate the S phase entry. Using dominant negative isoforms as well as selective pharmacological inhibitors we quantified S phase entry by nuclear labeling with bromodeoxyuridine. Only in astroglial cells harvested from the marginal zone of the neocortex inhibition of phosphoinositide-3-kinases reduced the nuclear labeling with bromodeoxyuridine, indicating that neocortical astroglial cells differ in the regulation of proliferation. The two kinase isoforms p110 and p110were essential for S phase entry. p110 diminished the level of the p27Kip1 which inactivates the complex of cyclin E and CDK2 necessary for entry into the S phase. p110phosphorylated and inhibited glycogen synthase kinase-3which can prevent S-phase entry. Taken together, both isoforms mediated S phase in a subgroup of neocortical astroglial cells and acted via distinct pathways.

  13. Aliupseereiden työssä oppiminen - Tapaustutkimus sosiomateriaalisten oppimisverkostojen näkökulmasta

    OpenAIRE

    Pekkarinen, Otto

    2017-01-01

    Tutkimuksessa tarkasteltiin Puolustusvoimien perusyksiköissä kouluttajatehtävissä toimivien aliupseerien työssä oppimista ja sen ohjaamista. Työssä oppimista tarkasteltiin kulttuurihistori-allisen toiminnan teorian ja tietoa luovan oppimisen metaforan mukaisen trialogisen oppimisen tarjoamilla teoreettisilla työkaluilla. Tutkimuksen teoreettinen näkökulma kiteytettiin sosiomate-riaalisen oppimisverkostoon, joka määriteltiin toiminnan yhteisen kohteen ympärille rakentu-neeksi yksilön, hänen yh...

  14. Työelämään valmentautumisen arvioinnin kehittäminen : Työhön ja itsenäiseen elämään valmentava ja kuntouttava opetus ja ohjaus

    OpenAIRE

    Wredfors, Marjo

    2013-01-01

    Keskuspuiston ammattiopisto on ORTON Invalidisäätiön ylläpitämä ammatillinen erityisoppilaitos ja erityisopetuksen kehittämiskeskus, joka tarjoaa muun muassa työhön ja itsenäiseen elämään valmentava ja kuntouttava opetus ja ohjaus -koulutusta (valmentava 2). Koulutuksen tavoitteena on antaa valmiuksia, joiden avulla opiskelija selviytyy jokapäiväisessä elämässä ja työpaikalla sijoittuen mahdollisimman itsenäiseen työtehtävään. Opinnäytetyön tarkoitus on kehittää työhön ja itsenäiseen elä...

  15. Perehdyttäminen työyhteisössä : Perehdyttämisopas Kartanogolfille

    OpenAIRE

    Tuovinen, Niina

    2013-01-01

    Tämän opinnäytetyön toimeksiantaja toimi Joroisten Kartano-Golf Oy. Kartanogolf on täysimittainen golfkenttä Joroisissa. Golfkenttä on avoinna huhtikuun lopusta lokakuulle. Kartanogolf työllistää kauden aikana 15–20 henkilöä, joista kolme vakituisesti. Suurin osa työsuhteista on määräaikaisia, joten työntekijöiden vaihtuvuus kausien välillä on suuri. Opinnäytetyön tavoitteena oli laatia perehdyttämisopas toimeksiantajalle perehdyttämisprosessin tueksi. Perehdyttämisoppaan laatimisen tavoi...

  16. TyG index, HOMA score and viral load in patients with chronic hepatitis C due to genotype 1.

    Science.gov (United States)

    Petta, S; Di Marco, V; Di Stefano, R; Cabibi, D; Cammà, C; Marchesini, G; Craxì, A

    2011-07-01

    The triglycerides × glucose (TyG) index is a recently proposed surrogate marker of insulin resistance (IR), calculated from fasting plasma triglyceride and glucose concentrations. We tested the host and viral factors associated with Tyg and homeostasis model assessment (HOMA) scores, comparing their associations with histological features and with sustained virological response (SVR) in patients with genotype 1 chronic hepatitis C(G1CHC). Three hundred and forty consecutive patients with G1CHC were considered. All had a liver biopsy scored by one pathologist for staging and grading (Scheuer), and graded for steatosis, which was considered moderate-severe if ≥30%. Anthropometric and metabolic measurements, including IR measured by both HOMA and TyG, were registered. By linear regression analysis, TyG was independently associated with waist circumference (WC), total cholesterol, presence of arterial hypertension, Log10 HCV-RNA and steatosis. Similarly, WC and steatosis were significantly associated with HOMA. Older age (OR, 1.036; 95%CI, 1.004-1.070, P = 0.02), higher WC (1.031; 1.004-1.060; P = 0.02) and higher TyG (11.496; 3.163-41.784; P HOMA-IR in the model, the latter remained significantly associated with steatosis ≥30% (1.237; 1.058-1.448; P = 0.008). Receiver operating characteristic curves showed a similar performance of TyG (AUC 0.682) and HOMA-IR (AUC 0.699) in predicting moderate-severe steatosis. No independent associations were found between both TyG and HOMA and fibrosis or SVR. In patients with G1CHC , TyG, an easy-to-calculate and low-cost surrogate marker of IR, is linked to liver steatosis and shows an independent association with viral load. © 2011 Blackwell Publishing Ltd.

  17. Characterization of Direct Push Vadose Zone Sediments from the T and TY Waste Management Areas

    Energy Technology Data Exchange (ETDEWEB)

    Brown, Christopher F.; Valenta, Michelle M.; Serne, R. Jeffrey; Bjornstad, Bruce N.; Lanigan, David C.; Iovin, Cristian; Clayton, Ray E.; Geiszler, Keith N.; Clayton, Eric T.; Kutnyakov, Igor V.; Baum, Steven R.; Lindberg, Michael J.; Orr, Robert D.

    2007-06-08

    This report contains all the geochemical and selected physical characterization data collected on vadose zone sediment recovered from 5 direct push characterization holes emplaced to investigate vadose zone contamination associated with leaks from tanks 241-TY-105 (UPR-200-W-152) and 241-TY-106 (UPR-200-W-153). Tank 241-TY-105 is estimated to have leaked 35,000 gal of tributyl phosphate (TBP) waste from the uranium recovery process to the vadose zone in 1960. Tank 241-TY-106 is estimated to have leaked 20,000 gal of TBP-uranium recovery waste to the vadose zone in 1959. Although several drywells in the vicinity of tank 241-TY-106 contain measurable quantities of cesium-137 and/or cobalt-60, their relatively low concentrations indicate that the contaminant inventory in the vadose zone around tank 241-TY-106 is quite small. Additionally, this report contains all the geochemical and selected physical characterization data collected on vadose zone sediment recovered from 7 direct push characterization holes emplaced to investigate vadose zone contamination associated with an overfill event and leak from tank 241-T-101.

  18. Kutsumuksesta palkkatyöhön? Perusasteen opettajan työn muuttunut luonne ja logiikka

    OpenAIRE

    Lindén, Jyri

    2010-01-01

    Kun opetustyön luonne muuttuu, myös sen mielekkyys on vaarassa kadota Opetustyö on vaarassa muuttua kutsumustyöstä pelkäksi palkkatyöksi, jossa työn mieltä ja merkitystä arvioidaan suhteessa siitä saatavaan taloudelliseen hyötyyn. Kun mahdollisuudet tehdä kutsumusperustaista työtä itseä tyydyttävällä tavalla heikkenevät merkittävästi, työntekijä alkaa kyseenalaistaa sitoutumisensa työhön ja sen kohteeseen. Opetustyön erityisasema on perustunut sille asetettuun tehtävään ylläpitää yhte...

  19. Interaktiivinen portfolio työelämälähtöisen opiskelun pelivälineenä

    OpenAIRE

    Kallioranta, Jussi

    2013-01-01

    Opinnäytetyön aiheena oli työelämälähtöisyyden kehittäminen opiskelussa pelillistämisen keinoin. Työssä tarkasteltiin opiskelijoiden työelämätaitoja, sekä portfoliota välineenä oman osaamisen tunnistamisessa. Lisäksi työssä pohdittiin korkeakoulujen ja yritysten välisen yhteistyön kehittämistä avoimen innovaation mallien avulla. Työn tuloksena syntyi idea interaktiivisesta verkottuvasta portfoliosta, joka auttaa opiskelijaa tunnistamaan osaamistaan ja verkottumaan muiden opiskelijoiden ka...

  20. Perehdytysopas Solo Sokos Hotel Tornin vastaanottoon uudelle työntekijälle

    OpenAIRE

    Plester, Sira

    2013-01-01

    Tämä opinnäytetyö on viitekehys perehdytysoppaalle Solo Sokos Hotel Tornin vastaanottoon. Perehdytysopas ohjeistaa vastaanoton uusia työntekijöitä työtehtävissä sekä tarjoaa tietoa jokapäiväisestä työssä Solo Sokos Hotel Tornin vastaanotossa ja sen ympäristössä. Tässä opinnäytetyössä pohditaan minkälainen hyvän perehdytysoppaan tulisi olla. Opinnäytetyössä pohditaan myös mitä tietoja perehdytysoppaaseen Solo Sokos Hotel Tornin vastaanottoon tarvitsee. Opinnäytetyössä käydään lisäksi l...

  1. HIV-1 stimulates nuclear entry of amyloid beta via dynamin dependent EEA1 and TGF-β/Smad signaling

    International Nuclear Information System (INIS)

    András, Ibolya E.; Toborek, Michal

    2014-01-01

    Clinical evidence indicates increased amyloid deposition in HIV-1-infected brains, which contributes to neurocognitive dysfunction in infected patients. Here we show that HIV-1 exposure stimulates amyloid beta (Aβ) nuclear entry in human brain endothelial cells (HBMEC), the main component of the blood–brain barrier (BBB). Treatment with HIV-1 and/or Aβ resulted in concurrent increase in early endosomal antigen-1 (EEA1), Smad, and phosphorylated Smad (pSmad) in nuclear fraction of HBMEC. A series of inhibition and silencing studies indicated that Smad and EEA1 closely interact by influencing their own nuclear entry; the effect that was attenuated by dynasore, a blocker of GTP-ase activity of dynamin. Importantly, inhibition of dynamin, EEA1, or TGF-β/Smad effectively attenuated HIV-1-induced Aβ accumulation in the nuclei of HBMEC. The present study indicates that nuclear uptake of Aβ involves the dynamin-dependent EEA1 and TGF-β/Smad signaling pathways. These results identify potential novel targets to protect against HIV-1-associated dysregulation of amyloid processes at the BBB level. - Highlights: • HIV-1 induces nuclear accumulation of amyloid beta (Aβ) in brain endothelial cells. • EEA-1 and TGF-Β/Smad act in concert to regulate nuclear entry of Aβ. • Dynamin appropriates the EEA-1 and TGF-Β/Smad signaling. • Dynamin serves as a master regulator of HIV-1-induced nuclear accumulation of Aβ

  2. HIV-1 stimulates nuclear entry of amyloid beta via dynamin dependent EEA1 and TGF-β/Smad signaling

    Energy Technology Data Exchange (ETDEWEB)

    András, Ibolya E., E-mail: iandras@med.miami; Toborek, Michal, E-mail: mtoborek@med.miami.edu

    2014-04-15

    Clinical evidence indicates increased amyloid deposition in HIV-1-infected brains, which contributes to neurocognitive dysfunction in infected patients. Here we show that HIV-1 exposure stimulates amyloid beta (Aβ) nuclear entry in human brain endothelial cells (HBMEC), the main component of the blood–brain barrier (BBB). Treatment with HIV-1 and/or Aβ resulted in concurrent increase in early endosomal antigen-1 (EEA1), Smad, and phosphorylated Smad (pSmad) in nuclear fraction of HBMEC. A series of inhibition and silencing studies indicated that Smad and EEA1 closely interact by influencing their own nuclear entry; the effect that was attenuated by dynasore, a blocker of GTP-ase activity of dynamin. Importantly, inhibition of dynamin, EEA1, or TGF-β/Smad effectively attenuated HIV-1-induced Aβ accumulation in the nuclei of HBMEC. The present study indicates that nuclear uptake of Aβ involves the dynamin-dependent EEA1 and TGF-β/Smad signaling pathways. These results identify potential novel targets to protect against HIV-1-associated dysregulation of amyloid processes at the BBB level. - Highlights: • HIV-1 induces nuclear accumulation of amyloid beta (Aβ) in brain endothelial cells. • EEA-1 and TGF-Β/Smad act in concert to regulate nuclear entry of Aβ. • Dynamin appropriates the EEA-1 and TGF-Β/Smad signaling. • Dynamin serves as a master regulator of HIV-1-induced nuclear accumulation of Aβ.

  3. Eihän "leikkitäti" voi väsyä, vai voiko? : Käytännön olosuhteet ja työstä aiheutuva stressi musiikkileikkikoulunopettajan työssä

    OpenAIRE

    Busk, Vilma

    2012-01-01

    TIIVISTELMÄ Tässä opinnäytetyössä tarkastellaan musiikkileikkikoulunopettajan työtä ja sen haasteita. Keskiössä ovat työn käytännön olosuhteet ja työstä aiheutuva stressi. Opinnäytetyötäni varten tein kyselytutkimuksen, jonka toteutin Internet-kyselynä Facebookissa, Muskari-ideoita-sivustolla. Kyselyyn vastasi 48 musiikkileikkikoulunopettajaa, joilla oli vaihteleva määrä kokemusta ja jotka olivat iältään 21—50-vuotiaita. Vastaajien taustatietojen lisäksi kartoitin mahdollisimman mon...

  4. Palkansaajasta itsensä työllistäjäksi : Naisyrittäjyyden näkökulmasta

    OpenAIRE

    Sirviö, Outi

    2016-01-01

    Opinnäytetyön aiheena on palkansaajasta itsensä työllistäjäksi naisyrittäjyyden näkökulmasta. Työssä kuvaillaan yrittäjyyden sekä naisyrittäjyyden keskeisiä piirteitä. Työn tarkoituksena on tutkia naisyrittäjyyden näkökulmasta yrityksen perustamiseen liittyvää toimintaa, verkostoja sekä rahoitusmahdollisuuksia. Työ on rajattu koskemaan naisyrittäjyyttä ja heidän mahdollisuuksiaan ja motiiveja työllistää itsensä oman yrityksen kautta. Suomessa on Naisyrittäjiä noin 80 000, joka on noin kol...

  5. Työntekijöiden ja lähiesimiehen välinen yhteistyö Kissanmaan perhetukikeskuksessa

    OpenAIRE

    Salonen, Jenni

    2009-01-01

    Opinnäytetyöni tavoitteena oli selvittää lähiesimiehen ja työntekijöiden välistä yhteistyötä tamperelaisen Kissanmaan perhetukikeskuksen lastenpäivystys- ja lastenpsykiatrisella kuntoutusosastolla. Tutkimuksessa selvitin yhteistyöaiheita ja mitkä asiat siinä sujuvat ja mitkä vaativat kehittämistä. Lisäksi selvitin, millaista tukea työntekijät lähiesimieheltään saavat. Tarkoitukseni oli myös selvittää, miten työntekijöiden kuuleminen ja työntekijän ja lähiesimiehen välinen yhteistyö liittyvät ...

  6. Selvitys HR House Oy:n vuokratyöntekijöiden työtyytyväisyydestä

    OpenAIRE

    Kaskela, Leeni

    2011-01-01

    Opinnäytetyön tarkoituksena oli selvittää HR House Oy:n palveluksessa olevien vuokratyöntekijöiden työtyytyväisyyteen vaikuttavia asioita. Opinnäytetyö on laadullinen tutkielma, jossa käytän tutkimusmenetelmänä teemahaastattelua. Tutkimuskysymykseni selvittävät, mitkä asiat vaikuttavat työntekijöiden työ- tyytyväisyyteen ja mitä he kertovat työtyytyväisyyteen vaikuttavista tekijöistä. Toimeksianto opinnäytetyön tekemiseen on tullut HR House Oy Henkilöstöpalveluilta. Tavoitteena oli löytää kei...

  7. Caracterização de genótipos de tomateiro resistentes a begomovírus por marcador molecular co-dominante ligado ao gene Ty-1 Fingerprinting of tomato genotypes resistant to begomovirus by a codominant molecular marker linked to Ty-1 gene

    Directory of Open Access Journals (Sweden)

    Daniela Aparecida de Castro Nizio

    2008-12-01

    Full Text Available O objetivo deste trabalho foi avaliar genótipos de tomateiro, quanto à resistência a begomovírus, e caracterizar, por meio do marcador molecular SSR-47, híbridos de tomate de mesa portadores do alelo de resistência ao begomovírus Ty-1, com potencial comercial. Os 24 híbridos experimentais, heterozigotos no loco Ty-1, depois de infectados via enxertia, apresentaram sintomas intermediários, em comparação aos identificados pelas linhagens homozigotas Ty-1/Ty-1 e pelos genótipos suscetíveis Ty-1+/Ty-1+, o que indica a dominância incompleta do alelo Ty-1. Esses híbridos foram considerados como parcialmente tolerantes a begomovírus. Os híbridos experimentais TEX-246, TEX-261, TEX-253, TEX-256, TEX-262, TEX-252, TEX-251 e TEX-268 aliaram médias elevadas de produção total e de massa média dos frutos; e os híbridos TEX-246, TEX-253, TEX-256, TEX-262 e TEX-252 apresentaram valores elevados também para meia-vida da firmeza e foram, portanto, considerados competitivos em comparação aos padrões comerciais usados como testemunhas. O marcador molecular SSR-47 foi eficiente em caracterizar genótipos portadores do alelo Ty-1. A infecção do begomovírus, induzida via enxertia, manifestou sintomas, nos genótipos testados, condizentes com os resultados obtidos com o marcador molecular SSR-47.The objectives of this study were to evaluate tomato genotypes for begomovirus resistance and to assess SSR-47 fingerprinting patterns associated with alleles Ty-1, in hybrids of fresh-market tomato with commercial potential. Twenty-four experimental genotypes, heterozygous at Ty-1 locus, were infected with begomovirus via grafting, and showed intermediate symptoms compared to the ones identified by homozygous Ty-1/Ty-1 lines and by susceptible genotypes Ty-1+/Ty-1+, which indicates the incomplete dominance of the Ty-1 allele. These hybrids were considered to possess partial resistance to begomovirus. The experimental hybrids TEX-246, TEX-261, TEX

  8. Työntekijän opas lapsen rakkaudelliseen kohtaamiseen Helsingin Diakonissalaitoksen intensiivihoidossa

    OpenAIRE

    Cacho, Janika

    2014-01-01

    TIIVISTELMÄ Cacho, Janika. Työntekijän opas lapsen rakkaudelliseen kohtaamiseen Helsingin Diakonissalaitoksen intensiivihoidossa. Kevät 2014, 73s., 2 liitteitä. Diakonia-ammattikorkeakoulu, Sosiaalialan koulutusohjelma, Kristillisen lapsi- ja nuorisotyön suuntautumisvaihtoehto, sosionomi (AMK) + kirkon nuorisotyöntekijän virkakelpoisuus. Opinnäytetyöni tavoitteena oli tuottaa opas, joka tarjoaisi Helsingin Diakonissalaitoksen intensiivihoidon työntekijöille tietoa lapsen rak...

  9. Introduction of the Comprehensive Nuclear-Test-Ban Treaty (CTBT) and preparatory activities for its entry into force

    Energy Technology Data Exchange (ETDEWEB)

    Tani, Hiroshi [Japan Atomic Energy Research Inst., Mutsu Establishment, Mutsu, Aomori (Japan)

    2001-03-01

    The Comprehensive Nuclear-Test-Ban Treaty (CTBT) is a very important treaty, not only for Japan but also for the world, because it prohibits any nuclear weapon test explosion or any other nuclear explosion anywhere in the world. The treaty however will not enter into force until it has been signed and ratified by all the 44 states listed in Annex 2 to the treaty. Many efforts to facilitate the treaty's early entry into force are being done by many countries and many international organizations. As one of result of these efforts, a Preparatory Commission for the Comprehensive Nuclear-Test-Ban Treaty Organization had be established at a meeting of State Signatories on 19 November 1996, and the Commission started activities to establish global verification regime of the treaty and to prepare for its entry into force. Under the CTBT activities, the Japan Atomic Energy Research Institute (JAERI) is expected to play an important role as supporter for the Japanese Government, especially in a field of an International Monitoring System (IMS). However, there is no appropriate guide book on the CTBT for JAERI staff at present. This report provides some introduction of the CTBT regime and preparatory activities for its entry into force. Only open source information is used for making the report. If anyone need more detail information, it should be asked to contact competent authorities. (author)

  10. Tuntityöntekijöiden työtyytyväisyyden mittaaminen - Case Yritys X

    OpenAIRE

    Länsilahti, Anni; Yliniemi, Sanna

    2013-01-01

    Työtyytyväisyyden mittaaminen suoritettiin suomalaisen finanssialan tuntityöntekijöille. Tuntityöntekijällä kyseenomaisessa yrityksessä tarkoitetaan osa-aikaista työntekijää. Tuntityöntekijöiden työnkuva keskittyy pääsääntöisesti uusasiakashankintaan sekä nykyisten asiakassuhteiden ylläpitämiseen vakuutusasioissa. Tuntityöntekijät tekevät keskimäärin noin 16 tuntia töitä viikossa Tutkimuksen kohteena olivat koko Suomen tuntityöntekijät, joita yrityksessä on noin 50 henkeä. Suurin osa tuntityö...

  11. Tank 241-TY-101 Tank Characterization Plan

    International Nuclear Information System (INIS)

    Homi, C.S.

    1995-01-01

    This document is a plan which serves as the contractual agreement between the Characterization Program, Sampling Operations, Oak Ridge National Laboratory, and PNL tank vapor program. The scope of this plan is to provide guidance for the sampling and analysis of vapor samples from tank 241-TY-101

  12. Vapor space characterization of Waste Tank 241-TY-104: Results from samples collected on 4/27/95

    International Nuclear Information System (INIS)

    Klinger, G.S.; Olsen, K.B.; Clauss, T.W.

    1995-10-01

    This report describes inorganic and organic analyses results from samples obtained from the headspace of the Hanford waste storage Tank 241-TY-104 (referred to as Tank TY-104). The results described here were obtained to support safety and toxicological evaluations. A summary of the results for inorganic and organic analytes is listed in Table 1. Detailed descriptions of the results appear in the text. Quantitative results were obtained for the inorganic compounds ammonia (NH 3 ), nitrogen dioxide (NO 2 ), nitric oxide (NO), and water (H 2 O). Sampling for hydrogen cyanide (HCN) and sulfur oxides (SO x ) was not requested. In addition, quantitative results were obtained for the 39 TO-14 compounds plus an additional 14 analytes. Of these, 8 were observed above the 5-ppbv reporting cutoff. Five tentatively identified compounds (TICs) were observed above the reporting cutoff of (ca.) 10 ppbv and are reported with concentrations that are semiquantitative estimates based on internal-standard response factors. The 10 organic analytes with the highest estimated concentrations are listed in Table 1 and account for approximately 94% of the total organic components in Tank TY-104. Nitrous oxide (N 2 O) was the only permanent gas detected in the tank-headspace samples. Tank TY-104 is on the Ferrocyanide Watch List

  13. Vapor space characterization of waste Tank 241-TY-101: Results from samples collected on 4/6/95

    International Nuclear Information System (INIS)

    Klinger, G.S.; Clauss, T.W.; Ligotke, M.W.; Pool, K.H.; McVeety, B.D.; Olsen, K.B.; Bredt, O.P.; Fruchter, J.S.; Goheen, S.C.

    1995-11-01

    This report describes inorganic and organic analyses results from samples obtained from the headspace of the Hanford waste storage Tank 241-TY-101 (referred to as Tank TY-101). The results described here were obtained to support safety and toxicological evaluations. A summary of the results for inorganic and organic analytes is listed in Table 1. Detailed descriptions of the results appear in the text. Quantitative results were obtained for the inorganic compounds ammonia (NH 3 ), nitrogen dioxide (NO 2 ), nitric oxide (NO), and water vapor (H 2 O). Sampling for hydrogen cyanide (HCN) and sulfur oxides (SO x ) was not requested. In addition, quantitative results were obtained for the 39 TO-14 compounds plus an additional 14 analytes. Off these, 5 were observed above the 5-ppbv reporting cutoff. One tentatively identified compound (TIC) was observed above the reporting cutoff of (ca.) 10 ppbv and are reported with concentrations that are semiquantitative estimates based on internal-standard response factors. The six organic analyses identified are listed in Table 1 and account for approximately 100% of the total organic components in Tank TY-101. Two permanent gases, carbon dioxide (CO 2 ) and nitrous oxide (N 2 O), were also detected. Tank TY-101 is on the Ferrocyanide Watch List

  14. Genetic modification of glaucoma associated phenotypes between AKXD-28/Ty and DBA/2J mice

    Directory of Open Access Journals (Sweden)

    Zabaleta Adriana

    2001-01-01

    Full Text Available Abstract Background Glaucoma is a common disease but its molecular etiology is poorly understood. It involves retinal ganglion cell death and optic nerve damage that is often associated with elevated intraocular pressure. Identifying genes that modify glaucoma associated phenotypes is likely to provide insights to mechanisms of glaucoma. We previously reported glaucoma in DBA/2J mice caused by recessive alleles at two loci, isa and ipd, that cause iris stromal atrophy and iris pigment dispersion, respectively. A approach for identifying modifier genes is to study the effects of specific mutations in different mouse strains. When the phenotypic effect of a mutation is modified upon its introduction into a new strain, crosses between the parental strains can be used to identify modifier genes. The purpose of this study was to determine if the effects of the DBA/2J derived isa and ipd loci are modified in strain AKXD-28/Ty. Results AKXD-28/Ty mice develop glaucoma characterized by intraocular pressure elevation, retinal ganglion loss, and optic nerve excavation. In AKXD-28/Ty, isa causes an iris stromal atrophy phenotype as in DBA/2J. However, the iris pigment dispersion phenotype associated with ipd in DBA/2J does not occur in AKXD-28/Ty. Additionally, a greater severity and speed of retinal and optic nerve damage following intraocular pressure elevation in AKXD-28/Ty compared to DBA/2J mice suggests that AKXD-28/Ty is more susceptible to pressure-induced cell death. Conclusions The consequences of the ipd and isa mutations are modified in the AKXD-28/Ty background. These strains provide a resource for the identification of modifier genes that modulate pigment dispersion and susceptibility to pressure-induced cell death.

  15. A role for the budding yeast separase, Esp1, in Ty1 element retrotransposition.

    Directory of Open Access Journals (Sweden)

    Krystina L Ho

    2015-03-01

    Full Text Available Separase/Esp1 is a protease required at the onset of anaphase to cleave cohesin and thereby enable sister chromatid separation. Esp1 also promotes release of the Cdc14 phosphatase from the nucleolus to enable mitotic exit. To uncover other potential roles for separase, we performed two complementary genome-wide genetic interaction screens with a strain carrying the budding yeast esp1-1 separase mutation. We identified 161 genes that when mutated aggravate esp1-1 growth and 44 genes that upon increased dosage are detrimental to esp1-1 viability. In addition to the expected cell cycle and sister chromatid segregation genes that were identified, 24% of the genes identified in the esp1-1 genetic screens have a role in Ty1 element retrotransposition. Retrotransposons, like retroviruses, replicate through reverse transcription of an mRNA intermediate and the resultant cDNA product is integrated into the genome by a conserved transposon or retrovirus encoded integrase protein. We purified Esp1 from yeast and identified an interaction between Esp1 and Ty1 integrase using mass spectrometry that was subsequently confirmed by co-immunoprecipitation analysis. Ty1 transposon mobility and insertion upstream of the SUF16 tRNA gene are both reduced in an esp1-1 strain but increased in cohesin mutant strains. Securin/Pds1, which is required for efficient localization of Esp1 to the nucleus, is also required for efficient Ty1 transposition. We propose that Esp1 serves two roles to mediate Ty1 transposition - one to remove cohesin and the second to target Ty1-IN to chromatin.

  16. Ensihoitotyön sosiaalinen kuormittavuus : – työhyvinvoinnin voimavara vai taakka?

    OpenAIRE

    Ahonen, Kaisa

    2015-01-01

    Opinnäytetyön tarkoituksena on kartoittaa ensihoitotyön sosiaalista kuormittavuutta eräässä aluepelastuslaitoksessa. Opinnäytetyön tavoitteena on tehdä tarvittavia kehittämisehdotuksia sosiaalisen kuormittavuuden hallitsemiseen organisaatiossa. Työn kuormittavuudella on suuri vaikutus työhyvinvointiin, jolla on merkitystä organisaation suorituskykyyn. Aineisto kerättiin kesäkuussa 2014 aikaisemmin kehitetyllä ja testatulla mittarilla. QPSNordic on pohjoismainen psyykkisten ja sosiaalisten...

  17. Antitumor activity of TY-011 against gastric cancer by inhibiting Aurora A, Aurora B and VEGFR2 kinases

    Directory of Open Access Journals (Sweden)

    Wang Liu

    2016-11-01

    Full Text Available Abstract Background Overexpression of Aurora A and B has been reported in a wide range of tumor types, including gastric cancer. Anti-angiogenesis has been considered as an important therapeutic modality in advanced gastric cancer. Here we identified a novel compound TY-011 with promising antitumor activity by targeting mitotic kinases (Aurora A and B and angiogenic receptor tyrosine kinase (VEGFR2. Methods HTRF® KinEASE™ assay was used to detect the effect of TY-011 against Aurora A, Aurora B and VEGFR2 activities. Docking simulation study was performed to predict the binding mode of TY-011 with Aurora A and B kinases. CCK-8 assay was used to test cell growth. Cell cycle and cell apoptosis was analyzed by flow cytometry. Gastric cancer cell xenograft mouse models were used for in vivo study. TUNEL kit was used to determine the apoptosis of tumor tissues. Immunohistochemistry analysis and HUVEC tube formation assay were performed to determine the anti-angiogenesis ability. Immunofluorescence and western blot were used to test protein expression. Results TY-011 was identified as a potential Aurora A and B inhibitor by HTRF® KinEASE™ assay. It effectively inhibited cellular Aurora A and B activities in a concentration-dependent manner. TY-011 occupied the ATP-binding site of both Aurora A and B kinases. TY-011 demonstrated prominent inhibitory effects on proliferation of gastric cancer cells. TY-011 treatment induced an obvious accumulation of cells at G2/M phase and a modest increase of cells with >4 N DNA content, which then underwent apoptosis. Meaningfully, orally administration of TY-011 demonstrated superior efficacy against the tumor growth in gastric cancer cell xenograft, with ~90% inhibition rate and 100% tumor regression at 9 mg/kg dose, and TY-011 did not affect the body weight of mice. Interestingly, we observed that TY-011 also antagonized tumor angiogenesis by targeting VEGFR2 kinase. Conclusions These results indicate that

  18. Perhetukikeskus Auerkulma : työntekijöiden näkemyksiä tiedon johtamisesta ja sen jakamisesta

    OpenAIRE

    Alho-Mikkonen, Heli

    2014-01-01

    Opinnäytetyön tarkoituksena oli kuvata Perhetukikeskus Auerkulman työntekijöiden näkemyksiä tiedon johtamisesta, jakamisesta ja jakamiseen vaikuttavista tekijöistä. Samalla tarkoituksena oli kuvata myös, miten tiedon jakamiseen tulisi varautua tulevaisuudessa ja miten tiedon jakamista voitaisiin tukea johtamisella. Tavoitteena oli saada tietoa työntekijöiden koulutustarpeista, jotta tulevaisuudessa tiedon johtamista ja jakamista olisi mahdollista kehittää. Tavoitteena oli myös työn rakenteide...

  19. Comparative analysis of pepper and tomato reveals euchromatin expansion of pepper genome caused by differential accumulation of Ty3/Gypsy-like elements

    Directory of Open Access Journals (Sweden)

    Ahn Jong Hwa

    2011-01-01

    Full Text Available Abstract Background Among the Solanaceae plants, the pepper genome is three times larger than that of tomato. Although the gene repertoire and gene order of both species are well conserved, the cause of the genome-size difference is not known. To determine the causes for the expansion of pepper euchromatic regions, we compared the pepper genome to that of tomato. Results For sequence-level analysis, we generated 35.6 Mb of pepper genomic sequences from euchromatin enriched 1,245 pepper BAC clones. The comparative analysis of orthologous gene-rich regions between both species revealed insertion of transposons exclusively in the pepper sequences, maintaining the gene order and content. The most common type of the transposon found was the LTR retrotransposon. Phylogenetic comparison of the LTR retrotransposons revealed that two groups of Ty3/Gypsy-like elements (Tat and Athila were overly accumulated in the pepper genome. The FISH analysis of the pepper Tat elements showed a random distribution in heterochromatic and euchromatic regions, whereas the tomato Tat elements showed heterochromatin-preferential accumulation. Conclusions Compared to tomato pepper euchromatin doubled its size by differential accumulation of a specific group of Ty3/Gypsy-like elements. Our results could provide an insight on the mechanism of genome evolution in the Solanaceae family.

  20. Uhka- ja väkivaltatilanteet hotellin vastaanottovirkailijan työssä

    OpenAIRE

    Malinen, Kristiina

    2009-01-01

    Opinnäytetyön tarkoitus on auttaa parantamaan hotellin vastaanotossa työskentelevän henkilöstön turvallisuutta ehkäisemällä ja hallitsemalla uhka- ja väkivaltatilanteita. Tutkimuksen keskeisiä käsitteitä ovat uhka- ja väkivaltatilanteet sekä asiakaspalvelutyö hotellin vastaanotossa. Väkivalta jaetaan fyysiseen ja psyykkiseen väkivaltaan sekä seksuaaliseen häirintään. Tutkimuksessa asiakaspalvelua tarkastellaan henkilökunnan työturvallisuuden näkökulmasta. Vastaanottovirkailija voi omalla ...

  1. Entry-Control Systems Handbook

    International Nuclear Information System (INIS)

    1978-09-01

    The function of an entry-control system in a total Physical Protection System is to allow the movement of authorized personnel and material through normal access routes, yet detect and delay unauthorized movement of personnel and material from uncontrolled areas. The ten chapters of this handbook cover: introduction, credentials, personnel identity verification systems, special nuclear materials monitors, metal detectors, explosives sensors, package search systems, criteria for selection of entry-control equipment, machine-aided manual entry-control systems, and automated entry-control systems. A system example and its cost are included as an appendix

  2. Flexible robotic entry device for a nuclear materials production reactor

    International Nuclear Information System (INIS)

    Heckendorn, F.M. II.

    1988-01-01

    The Savannah River Laboratory has developed and is implementing a flexible robotic entry device (FRED) for the nuclear materials production reactors now operating at the Savannah River Plant (SRP). FRED is designed for rapid deployment into confinement areas of operating reactors to assess unknown conditions. A unique smart tether method has been incorporated into FRED for simultaneous bidirectional transmission of multiple video/audio/control/power signals over a single coaxial cable. This system makes it possible to use FRED under all operating and standby conditions, including those where radio/microwave transmissions are not possible or permitted, and increases the quantity of data available

  3. Application of Entry-Time Processes to Asset Management in Nuclear Power Plants

    International Nuclear Information System (INIS)

    Nelson, Paul; Wang, Shuwen; Kee, Ernie J.

    2006-01-01

    The entry-time approach to dynamic reliability is based upon computational solution of the Chapman-Kolmogorov (generalized state-transition) equations underlying a certain class of marked point processes. Previous work has verified a particular finite-difference approach to computational solution of these equations. The objective of this work is to illustrate the potential application of the entry-time approach to risk-informed asset management (RIAM) decisions regarding maintenance or replacement of major systems within a plant. Results are presented in the form of plots, with replacement/maintenance period as a parameter, of expected annual revenue, along with annual variance and annual skewness as indicators of associated risks. Present results are for a hypothetical system, to illustrate the capability of the approach, but some considerations related to potential application of this approach to nuclear power plants are discussed. (authors)

  4. Vapor space characterization of Waste Tank 241-TY-104 (in situ): Results from samples collected on 8/5/94

    International Nuclear Information System (INIS)

    Ligotke, M.W.; Pool, K.H.; Lucke, R.B.

    1995-10-01

    This report describes inorganic and organic analyses results from in situ samples obtained from the headspace of the Hanford waste storage Tank 241-TY-104 (referred to as Tank TY-104). The results described here were obtained to support safety and toxicological evaluations. A summary of the results for inorganic and organic analytes is listed in Table 1. Detailed descriptions of the results appear in the text. Quantitative results were obtained for the inorganic compounds ammonia (NH 3 ), nitrogen dioxide (NO 2 ), nitric oxide (NO), and water (H 2 O). Sampling for hydrogen cyanide (HCN) and sulfur oxides (SO x ) was not performed. In addition, the authors looked for the 39 TO-14 compounds plus an additional 14 analytes. Of these, eight were observed above the 5-ppbv reporting cutoff. Twenty-four organic tentatively identified compounds (TICs) were observed above the reporting cutoff of (ca.) 10 ppbv and are reported with concentrations that are semiquantitative estimates based on internal standard response factors. The 10 organic analytes with the highest estimated concentrations are listed in Table 1 and account for approximately 86% of the total organic components in Tank TY-104. Tank TY-104 is on the Ferrocyanide Watch List

  5. Verkkoyhteisöpalveluiden käytön hyödyt ja haitat työllistyvyydelle

    OpenAIRE

    Toivonen, Julia

    2016-01-01

    Sosiaalisen median käyttö on kasvanut viime vuosina huomattavasti ja verkkoyhteisöpalveluiden käyttö työllistyvyyden edistämisen apuna on kasvussa. Verkkoyhteisöpalveluiden käytöllä voidaan katsoa olevan sekä positiivia että negatiivisia vaikutuksia yksilön työllistyvyydelle. Sosiaalisella medialla on suuri mahdollisuus toimia erityisen tehokkaana osana yksilön työllistyvyyden edistämistä, sillä sosiaalinen media tarjoaa paljon mahdollisuuksia rekrytointiin, omien taitojen esille tuomiseen se...

  6. HIV-1 uncoating: connection to nuclear entry and regulation by host proteins

    Energy Technology Data Exchange (ETDEWEB)

    Ambrose, Zandrea, E-mail: zaa4@pitt.edu [Division of Infectious Diseases, Department of Medicine, University of Pittsburgh, School of Medicine, Pittsburgh, PA 15261 (United States); Aiken, Christopher [Department of Pathology, Microbiology and Immunology, Vanderbilt University, School of Medicine, Nashville, TN 37232 (United States)

    2014-04-15

    The RNA genome of human immunodeficiency virus type 1 (HIV-1) is enclosed by a capsid shell that dissociates within the cell in a multistep process known as uncoating, which influences completion of reverse transcription of the viral genome. Double-stranded viral DNA is imported into the nucleus for integration into the host genome, a hallmark of retroviral infection. Reverse transcription, nuclear entry, and integration are coordinated by a capsid uncoating process that is regulated by cellular proteins. Although uncoating is not well understood, recent studies have revealed insights into the process, particularly with respect to nuclear import pathways and protection of the viral genome from DNA sensors. Understanding uncoating will be valuable toward developing novel antiretroviral therapies for HIV-infected individuals.

  7. ”SUN SYY ETTÄ OON NYT TÄSSÄ” : Kuntouttavaan työtoiminnan merkitys ihmisen hyvinvoinnille ja mielenterveydelle.

    OpenAIRE

    Ylimäki, Titta

    2015-01-01

    Ylimäki, Titta. Kuntouttavan työtoiminnan merkitys ihmisen hyvinvoinnille ja mielenterveydelle. Diakonia-ammattikorkeakoulu, Oulu, kevät 2015 39 s., 2 liitettä. Terveyden edistämisen koulutusohjelma, Sosiaali- ja terveysalan ylempi AMK -tutkinto, Sosionomi (ylempi AMK). Tutkimuksen tavoitteena oli selvittää, millainen merkitys kuntouttavalla työtoiminnalla on siihen osallistuvien ihmisten mielestä. Tutkimuskysymykset olivat seuraavat: Mikä merkitys kuntouttavalla työtoiminnalla on ih...

  8. Phosphoinositide-3 kinase-Akt pathway controls cellular entry of Ebola virus.

    Directory of Open Access Journals (Sweden)

    Mohammad F Saeed

    2008-08-01

    Full Text Available The phosphoinositide-3 kinase (PI3K pathway regulates diverse cellular activities related to cell growth, migration, survival, and vesicular trafficking. It is known that Ebola virus requires endocytosis to establish an infection. However, the cellular signals that mediate this uptake were unknown for Ebola virus as well as many other viruses. Here, the involvement of PI3K in Ebola virus entry was studied. A novel and critical role of the PI3K signaling pathway was demonstrated in cell entry of Zaire Ebola virus (ZEBOV. Inhibitors of PI3K and Akt significantly reduced infection by ZEBOV at an early step during the replication cycle. Furthermore, phosphorylation of Akt-1 was induced shortly after exposure of cells to radiation-inactivated ZEBOV, indicating that the virus actively induces the PI3K pathway and that replication was not required for this induction. Subsequent use of pseudotyped Ebola virus and/or Ebola virus-like particles, in a novel virus entry assay, provided evidence that activity of PI3K/Akt is required at the virus entry step. Class 1A PI3Ks appear to play a predominant role in regulating ZEBOV entry, and Rac1 is a key downstream effector in this regulatory cascade. Confocal imaging of fluorescently labeled ZEBOV indicated that inhibition of PI3K, Akt, or Rac1 disrupted normal uptake of virus particles into cells and resulted in aberrant accumulation of virus into a cytosolic compartment that was non-permissive for membrane fusion. We conclude that PI3K-mediated signaling plays an important role in regulating vesicular trafficking of ZEBOV necessary for cell entry. Disruption of this signaling leads to inappropriate trafficking within the cell and a block in steps leading to membrane fusion. These findings extend our current understanding of Ebola virus entry mechanism and may help in devising useful new strategies for treatment of Ebola virus infection.

  9. ”Kaikki lähtee siitä, et ihmisillä on hyviä, itselleen sopivia, itseään kehittäviä työtehtäviä” Käsityksiä osaamisesta ja osaamisen kehittämisestä sekä työhyvinvoinnista asiantuntijatyössä

    OpenAIRE

    Kurru, Niina

    2015-01-01

    Tavoitteet. Tämän laadullisen tutkimuksen tavoitteena oli tarkastella käsityksiä osaamisesta, osaamisen kehittämisestä sekä työhyvinvoinnista asiantuntijatyössä työntekijöiden näkökulmasta. Lisäksi tavoitteena oli tarkastella näiden ilmiöiden kytkeytymistä toisiinsa. Aiemmat tutkimukset ovat osoittaneet, että osaaminen on keskeinen työhyvinvoinnin tekijä, ja osaamisen kehittämisellä on positiivisia vaikutuksia työhyvinvointiin erityisesti tietointensiivisessä työssä. Aihe on tärkeä, sillä asi...

  10. Novel Acetone Metabolism in a Propane-Utilizing Bacterium, Gordonia sp. Strain TY-5▿

    Science.gov (United States)

    Kotani, Tetsuya; Yurimoto, Hiroya; Kato, Nobuo; Sakai, Yasuyoshi

    2007-01-01

    In the propane-utilizing bacterium Gordonia sp. strain TY-5, propane was shown to be oxidized to 2-propanol and then further oxidized to acetone. In this study, the subsequent metabolism of acetone was studied. Acetone-induced proteins were found in extracts of cells induced by acetone, and a gene cluster designated acmAB was cloned on the basis of the N-terminal amino acid sequences of acetone-induced proteins. The acmA and acmB genes encode a Baeyer-Villiger monooxygenase (BVMO) and esterase, respectively. The BVMO encoded by acmA was purified from acetone-induced cells of Gordonia sp. strain TY-5 and characterized. The BVMO exhibited NADPH-dependent oxidation activity for linear ketones (C3 to C10) and cyclic ketones (C4 to C8). Escherichia coli expressing the acmA gene oxidized acetone to methyl acetate, and E. coli expressing the acmB gene hydrolyzed methyl acetate. Northern blot analyses revealed that polycistronic transcription of the acmAB gene cluster was induced by propane, 2-propanol, and acetone. These results indicate that the acmAB gene products play an important role in the metabolism of acetone derived from propane oxidation and clarify the propane metabolism pathway of strain TY-5 (propane → 2-propanol → acetone → methyl acetate → acetic acid + methanol). This paper provides the first evidence for BVMO-dependent acetone metabolism. PMID:17071761

  11. Nuclear Computerized Library for Assessing Reactor Reliability (NUCLARR): Guide to data processing and revision: Part 3, Hardware component failure data entry and revision procedures

    International Nuclear Information System (INIS)

    Gilmore, W.E.; Gertman, D.I.; Gilbert, B.G.; Reece, W.J.

    1988-11-01

    The Nuclear Computerized Library for Assessing Reactor Reliability (NUCLARR) is an automated data base management system for processing and storing human error probability (HEP) and hardware component failure data (HCFD). The NUCLARR system software resides on an IBM (or compatible) personal micro-computer. Users can perform data base searches to furnish HEP estimates and HCFD rates. In this manner, the NUCLARR system can be used to support a variety of risk assessment activities. This volume, Volume 3 of a 5-volume series, presents the procedures used to process HEP and HCFD for entry in NUCLARR and describes how to modify the existing NUCLARR taxonomy in order to add equipment types of action verbs. Volume 3 also specifies the various roles of the administrative staff on assignment to the NUCLARR Clearinghouse who are tasked with maintaining the data base, dealing with user requests, and processing NUCLARR data

  12. The Gpn3 Q279* cancer-associated mutant inhibits Gpn1 nuclear export and is deficient in RNA polymerase II nuclear targeting.

    Science.gov (United States)

    Barbosa-Camacho, Angel A; Méndez-Hernández, Lucía E; Lara-Chacón, Bárbara; Peña-Gómez, Sonia G; Romero, Violeta; González-González, Rogelio; Guerra-Moreno, José A; Robledo-Rivera, Angélica Y; Sánchez-Olea, Roberto; Calera, Mónica R

    2017-11-01

    Gpn3 is required for RNA polymerase II (RNAPII) nuclear targeting. Here, we investigated the effect of a cancer-associated Q279* nonsense mutation in Gpn3 cellular function. Employing RNAi, we replaced endogenous Gpn3 by wt or Q279* RNAi-resistant Gpn3R in epithelial model cells. RNAPII nuclear accumulation and transcriptional activity were markedly decreased in cells expressing only Gpn3R Q279*. Wild-type Gpn3R localized to the cytoplasm but a fraction of Gpn3R Q279* entered the cell nucleus and inhibited Gpn1-EYFP nuclear export. This property and the transcriptional deficit in Gpn3R Q279*-expressing cells required a PDZ-binding motif generated by the Q279* mutation. We conclude that an acquired PDZ-binding motif in Gpn3 Q279* caused Gpn3 nuclear entry, and inhibited Gpn1 nuclear export and Gpn3-mediated RNAPII nuclear targeting. © 2017 Federation of European Biochemical Societies.

  13. Secretion of non-cell-bound phytase by the yeast Pichia kudriavzevii TY13.

    Science.gov (United States)

    Hellström, A; Qvirist, L; Svanberg, U; Veide Vilg, J; Andlid, T

    2015-05-01

    Mineral deficiencies cause several health problems in the world, especially for populations consuming cereal-based diets rich in the anti-nutrient phytate. Our aim was to characterize the phytate-degrading capacity of the yeast Pichia kudriavzevii TY13 and its secretion of phytase. The phytase activity in cell-free supernatants from cultures with 100% intact cells was 35-190 mU ml(-1) depending on the media. The Km was 0.28 mmol l(-1) and the specific phytase activity 0.32 U mg(-1) total protein. The phytase activity and secretion of extracellular non-cell-bound phytase was affected by the medium phosphate concentrations. Further, addition of yeast extract had a clearly inducing effect, resulting in over 60% of the cultures total phytase activity as non-cell-bound. Our study reveals that it is possible to achieve high extracellular phytase activity from the yeast P. kudriavzevii TY13 by proper composition of the growth medium. TY13 could be a promising future starter culture for fermented foods with improved mineral bioavailability. Using strains that secrete phytase to the food matrix may significantly improve the phytate degradation by facilitating the enzyme-to-substrate interaction. The secreted non-cell-bound phytase activities by TY13 could further be advantageous in industrial production of phytase. © 2015 The Society for Applied Microbiology.

  14. HEVOSHETKET-VALOKUVAKIRJA : Pedagoginen dokumentointi työvälineenä sosiaalipedagogisessa hevostoiminnassa

    OpenAIRE

    Nikola, Riikka

    2012-01-01

    TIIVISTELMÄ Nikola, Riikka. Hevoshetket-valokuvakirja – pedagoginen dokumentointi työvälineenä sosiaalipedagogisessa hevostoiminnassa. Diak Itä, Pieksämäki kevät 2012. (37 s, 3 liitettä) Diakonia-ammattikorkeakoulu, Diak Itä, Pieksämäki. Sosiaalialan koulutusohjelma. Sosionomi (AMK). Opinnäytetyö on kehityspainotteinen produktio, joka koostuu 15 toimintakerrasta sosiaalipedagogista hevostoimintaa ja kolmesta sosiaalipedagogisen hevostoiminnan asiakkaalle yksilöllisesti kootusta Hevos...

  15. LoRa IoT -radion soveltuvuus käytettyjen työkoneiden tiedonsiirtoon

    OpenAIRE

    Hasu, Lassi

    2017-01-01

    Opinnäytetyön tarkoituksena oli tutkia LoRa-radiotekniikan soveltuvuutta lastinkäsittelylaitteiden tuottaman datan tiedonsiirtoon. LoRa on teollisen internetin sovelluksia varten kehitetty pitkän kantaman radiotekniikka. Tavoitteena oli löytää käyttötapauksia, joihin LoRa-tekniikka soveltuu etsittäessä kustannustehokasta radiotekniikkaa erityisesti käytettyihin lastinkäsittelylaitteisiin. Työn toimeksiantaja oli Cargotec Finland Oy:n Kalmar-liiketoimintayksikkö. Työn tutkimusmenetelmänä käyte...

  16. T-TY Tank Farm Interim Surface Barrier Demonstration - Vadose Zone Monitoring Plan

    International Nuclear Information System (INIS)

    Zhang, Z.F.; Strickland, Christopher E.; Field, Jim G.; Parker, Danny L.

    2010-01-01

    The Hanford Site has 149 underground single-shell tanks that store hazardous radioactive waste. Many of these tanks and their associated infrastructure (e.g., pipelines, diversion boxes) have leaked. Some of the leaked waste has entered the groundwater. The largest known leak occurred from the T-106 Tank of the 241-T Tank Farm in 1973. Five tanks are assumed to have leaked in the TY Farm. Many of the contaminants from those leaks still reside within the vadose zone within the T and TY Tank Farms. The Department of Energy's Office of River Protection seeks to minimize the movement of these contaminant plumes by placing interim barriers on the ground surface. Such barriers are expected to prevent infiltrating water from reaching the plumes and moving them further. The soil water regime is monitored to determine the effectiveness of the interim surface barriers. Soil-water content and water pressure are monitored using off-the-shelf equipment that can be installed by the hydraulic hammer technique. Four instrument nests were installed in the T Farm in fiscal year (FY) 2006 and FY2007; two nests were installed in the TY Farm in FY2010. Each instrument nest contains a neutron probe access tube, a capacitance probe, and four heat-dissipation units. A meteorological station has been installed at the north side of the fence of the T Farm. This document summarizes the monitoring methods, the instrument calibration and installation, and the vadose zone monitoring plan for interim barriers in T farm and TY Farm.

  17. TyG Index Change Is More Determinant for Forecasting Type 2 Diabetes Onset Than Weight Gain.

    Science.gov (United States)

    Navarro-González, David; Sánchez-Íñigo, Laura; Fernández-Montero, Alejandro; Pastrana-Delgado, Juan; Martinez, Jose Alfredo

    2016-05-01

    The risk of type 2 diabetes associated with obesity appears to be influenced by other metabolic abnormalities, and there is controversy about the harmless condition of the metabolically healthy obese (MHO) state. The aim of this study is to assess the risk of diabetes and the impact of changes in weight and in triglyceride-glucose index (TyG index), according to the metabolic health and obesity states.We analyzed prospective data of the Vascular Metabolic CUN cohort, a population-based study among a White European population (mean follow-up, 8.9 years). Incident diabetes was assessed in 1923 women and 3016 men with a mean age at baseline of 55.33 ± 13.68 and 53.78 ± 12.98 years old.A Cox proportional-hazard analysis was conducted to estimate the hazard ratio (HR) of diabetes on metabolically healthy nonobese (MHNO), metabolically healthy obese, metabolically unhealthy nonobese (MUNO), and metabolically unhealthy obese (MUO). A continuous standardized variable (z-score) was derived to compute the HR for diabetes per 1-SD increment in the body mass index (BMI) and the TyG index.MHO, MUNO, and MUO status were associated with the development of diabetes, HR of 2.26 (95% CI: 1.25-4.07), 3.04 (95% CI: 1.69-5.47), and 4.04 (95% CI: 2.14-7.63), respectively. MUNO individuals had 1.82 greater risk of diabetes compared to MHO subjects (95% CI: 1.04-3.22). The HRs for incident diabetes per 1-SD increment in BMI and TyG indexes were 1.23 (95% CI: 1.04-1.44) and 1.54 (95% CI: 1.40-1.68). The increase in BMI did not raise the risk of developing diabetes among metabolically unhealthy subjects, whereas increasing the TyG index significantly affect the risk in all metabolic health categories.Metabolic health is more important determinant for diabetes onset than weight gain. The increase in weight does not raise the risk of developing diabetes among metabolically unhealthy subjects.

  18. Työntekijärekisterin luominen ja käyttöönotto

    OpenAIRE

    Kurkola, Matias

    2010-01-01

    Logistiikan turvallisuudesta huolehtiminen on tärkeä osa kuljetuksiin erikoistuneiden yritysten toimintaa. Yleisesti käytössä olevien turvallisuustyökalujen, kuten esimerkiksi kulunvalvonnan lisäksi toiminnan laatu vaatii tarkkaa henkilö- ja tavarakontrollia. Tämän opinnäytetyön tavoitteena oli luoda TNT Suomi Oy:n Turun toimipisteeseen helppokäyttöinen työntekijärekisteri, joka vähentäisi esimiesten työmäärää vapauttamalla resursseja henkilöstöhallinnosta varsinaiseen esimiestyöhön. Yrity...

  19. Etätyökäytäntöjen kehittäminen

    OpenAIRE

    Haverinen, Liisa

    2017-01-01

    Opinnäytetyön tavoitteena oli luoda selvitys kohdeyritykselle siihen, kannattaako yrityksessä tehdä etätöitä laajemmin. Lähtötilanne oli, että etätöitä tehdään yrityksessä osittain. Etätöitä tekeviä työntekijöitä on kuitenkin vähän, ja etätöitä tehdään myös määrällisesti vähän. Työn alussa kerrotaan etätyöstä ilmiönä ja käsitteenä. Teoriaosuudessa selvitetään vastuita, hyötyjä ja haasteita liittyen etätöihin. Selvitetään myös etäjohtamisen ominaispiirteitä. Teoriaosuudessa käydään läpi e...

  20. RED ONE kuvaajan työkaluna

    OpenAIRE

    Käki, Ossi

    2010-01-01

    Opinnäytetyöni projektiosa, 30-minuuttinen lyhytelokuva Huomisen Muisto – Memory of Tomorrow kuvattiin RED ONE -kameralla vuosina 2008-2009. Opinnäytetyöni kirjallinen osuus tutkii miten RED ONE soveltuu kuvaajan työvälineeksi lyhytelokuvassa. Huomisen Muisto oli ensimmäinen Tampereen ammattikorkeakoulussa RED ONE:lla kuvattu elokuva ja koko tekninen workflow piti kehittää lähes nollasta. Paljon kuvan digitaalista jälkikäsittelyä sisältävänä science fiction -elokuvana Huomisen Muisto asetti i...

  1. Estudio de tolerancia local de la vacuna vax-TyVi® en ratas Sprague Dawley

    Directory of Open Access Journals (Sweden)

    Eligio Sosa Roble

    2005-05-01

    Full Text Available La vacuna antitifoídica vax-TyVi® consiste en una preparación de polisacárido capsular Vi de Salmonella typhi, el cual es diluido en una solución buffer isotónica solución amortiguador, a la que se le añade fenol como preservo. Cada dosis de 0,5 mL contiene 25 μg de polisacárido como sustancia activa. En nuestro país el esquema de vacunación contra la fiebre tifoidea con vax- TyVi® se aplica a los alumnos de 9-10 años (5to grado, una 2da dosis a la edad de 12-13 años (8vo grado y una 3ra dosis a la edad de 16-17 años (11no grado. Además, es vacunado el personal de riesgo de Salud Pública y el personal que manipula alimentos. En el presente trabajo se describe el ensayo de tolerancia local llevado a cabo con la vacuna vax-TyVi® durante su fase de estudios preclínicos, actualmente utilizada en la vacunación contra la fiebre tifoidea en Cuba. Se empleó un total de 170 ratas que fueron tratadas con la vacuna, su placebo (todos los componentes, excepto la materia prima activa, o que no recibieron tratamiento alguno (controles. Se realizaron observaciones clínicas diarias durante todo el ensayo, se determinó el consumo de agua y alimentos y se realizaron investigaciones anatomopatológicas a animales sacrificados 3, 7, 14, 21, 28, 35 y 42 días después de la inoculación. No se observaron muertes ni síntomas de toxicidad; no se encontraron diferencias estadísticamente significativas entre los pesos vivos, el consumo de agua ni el de alimentos entre los grupos del ensayo. Tampoco se observaron lesiones anatomopatológicas que indicaran toxicidad por parte del producto inoculado. Los resultados permitieron concluir que la potencialidad de la vacuna vax-TyVi® para producir efectos adversos locales es baja.

  2. Facebook Ravintola Sipulin markkinoinnin työkaluna

    OpenAIRE

    Eerikäinen, Hanna

    2014-01-01

    Tämä opinnäytetyö keskittyy tutkimaan sosiaalisen median kanavaa, Facebookia ja sen erityispiirteitä markkinoinnin näkökulmasta. Toimeksiantona Royal Ravintolat Oy:n yksikölle, Ravintola Sipulille, luotiin uudet Facebook-sivut sekä ohjeistus henkilökun-nalle sivujen käyttöä varten. Ohjeistus on tehty Royal Ravintoloiden yleisten ohjeistus-ten pohjalta erityisesti Ravintola Sipulin toimintaa koskevaksi. Työn tavoitteena on kehittää tietoperustan pohjalta Ravintola Sipulin markkinointia ja ...

  3. Työympäristön vaikutus toimistotyöntekijän tuottavuuteen

    OpenAIRE

    Bågman, Tomas

    2012-01-01

    Opinnäytetyössä tutkittiin fyysisten ympäristötekijöiden vaikutuksia toimistotyötä tekevän työntekijän tuottavuuteen ja suunniteltiin Pikval Oy:n suunnitteluosastolle uusi layout. Työntekijän tuottavuuteen vaikuttavia asioita ympäristön suhteen ovat rakennus, sisäilmasto, akustiset olot, valaistus ja niiden alaosiot. Melu ja keskeytykset osoittautuivat olevan merkittäviä tuottavuuteen vaikuttavia tekijöitä joihin tulisi kiinnittää huomiota toimiston layoutia suunnitellessa. Avoin ...

  4. Vapor space characterization of waste tank 241-TY-103: Results from samples collected on 4/11/95

    International Nuclear Information System (INIS)

    Ligotke, M.W.; Clauss, T.W.; Pool, K.H.

    1995-10-01

    This report describes inorganic and organic analyses results from samples obtained from the headspace of the Hanford waste storage Tank 241-TY-103 (referred to as Tank TY-103). The results described here were obtained to support safety and toxicological evaluations. A summary of the results for inorganic and organic analytes is listed in Table 1. Detailed descriptions of the results appear in the text. Quantitative results were obtained for the inorganic compounds ammonia (NH 3 ), nitrogen dioxide (NO 2 ), nitric oxide (NO), and water (H 2 O). Sampling for hydrogen cyanide (HCN) and sulfur oxides (SO x ) was not requested. In addition, quantitative results were obtained for the 39 TO-14 compounds plus an additional 14 analytes. Of these, 16 were observed above the 5-ppbv reporting cutoff. Sixteen tentatively identified compounds (TICs) were observed above the reporting cutoff of (ca.) 10 ppbv and are reported with concentrations that are semiquantitative estimates based on internal-standard response factors. The 10 organic analytes with the highest estimated concentrations are listed in Table 1 and account for approximately 95% of the total organic components in Tank TY-103. Two permanent gases, carbon dioxide (CO 2 ) and nitrous oxide (N 2 O), were also detected

  5. Kiinteistömuuntamon Työohje

    OpenAIRE

    Rinkinen, Heikki

    2010-01-01

    Kiinteistömuuntamo on kaupunkialueen sähkönjakelussa suosittu vaihtoehto. Sillä saadaan sähkönjakelulaitteisto pois näkyvistä ja arvokasta tonttitilaa jää muuhun rakennuskäyttöön. Kiinteistömuuntamo on määräyksiltään tiukempi kuin missä tahansa muualla sijaitsevat muuntamot. Työni on avuksi vastaavanlaisen kiinteistömuuntamon rakentamisessa. Opinnäytetyön oli tarkoitus koota määräyksiä ja ohjeistuksia koskien kiinteistömuuntamon suunnittelua rakentamista. Kerron myös opinnäytetyökohteen ...

  6. Ammattiosaamisen näytöt : Pohjois-Karjalan Ammattiopisto Outokumpu, Radio- ja TV-työ

    OpenAIRE

    Dufva, Pilvi; Laatikainen, Mika

    2008-01-01

    Kehittämishankeraportissa kuvataan Pohjois-Karjalan Ammattiopisto Outokummun Radio- ja TV-työn linjan ensimmäisten ammattiosaamisen näyttöjen suunnittelua ja toteuttamista. Ammattiosaamisen näytöt toteutettiin maaliskuussa 2008. Kehittämishankkeen aiheena oli suunnitella ja toteuttaa Radio- ja TV-työn ensimmäiset ammattiosaamisen näytöt video-, televisio- ja elokuvailmaisun 15 opintoviikon kokonaisuuteen (VTE-ilmaisu). This project describes how the first vocational skills demonstration wa...

  7. TyG Index Change Is More Determinant for Forecasting Type 2 Diabetes Onset Than Weight Gain

    Science.gov (United States)

    Navarro-González, David; Sánchez-Íñigo, Laura; Fernández-Montero, Alejandro; Pastrana-Delgado, Juan; Martinez, Jose Alfredo

    2016-01-01

    Abstract The risk of type 2 diabetes associated with obesity appears to be influenced by other metabolic abnormalities, and there is controversy about the harmless condition of the metabolically healthy obese (MHO) state. The aim of this study is to assess the risk of diabetes and the impact of changes in weight and in triglyceride-glucose index (TyG index), according to the metabolic health and obesity states. We analyzed prospective data of the Vascular Metabolic CUN cohort, a population-based study among a White European population (mean follow-up, 8.9 years). Incident diabetes was assessed in 1923 women and 3016 men with a mean age at baseline of 55.33 ± 13.68 and 53.78 ± 12.98 years old. A Cox proportional-hazard analysis was conducted to estimate the hazard ratio (HR) of diabetes on metabolically healthy nonobese (MHNO), metabolically healthy obese, metabolically unhealthy nonobese (MUNO), and metabolically unhealthy obese (MUO). A continuous standardized variable (z-score) was derived to compute the HR for diabetes per 1-SD increment in the body mass index (BMI) and the TyG index. MHO, MUNO, and MUO status were associated with the development of diabetes, HR of 2.26 (95% CI: 1.25–4.07), 3.04 (95% CI: 1.69–5.47), and 4.04 (95% CI: 2.14–7.63), respectively. MUNO individuals had 1.82 greater risk of diabetes compared to MHO subjects (95% CI: 1.04–3.22). The HRs for incident diabetes per 1-SD increment in BMI and TyG indexes were 1.23 (95% CI: 1.04–1.44) and 1.54 (95% CI: 1.40–1.68). The increase in BMI did not raise the risk of developing diabetes among metabolically unhealthy subjects, whereas increasing the TyG index significantly affect the risk in all metabolic health categories. Metabolic health is more important determinant for diabetes onset than weight gain. The increase in weight does not raise the risk of developing diabetes among metabolically unhealthy subjects. PMID:27175686

  8. Granulometric data 241-TY Tank Farm monitoring well sediments

    International Nuclear Information System (INIS)

    Fecht, K.R.; Price, W.H.

    1977-12-01

    Approximately 200 sediment samples collected during the drilling of wells in the 241-TY Tank Farm have been analyzed for grain size and calcium carbonate content. The grain size data were used to categorize the sediment samples into sediment classes. The granulometric data, the calcium carbonate data, and the sediment class of each of the 200 sediment samples are documented in this paper

  9. Terminologia työvälineenä – aikamuotojärjestelmästä ja sen opetuskäytänteiden ergonomiasta S2-opetuksessa

    Directory of Open Access Journals (Sweden)

    Maria Kok

    2012-10-01

    Full Text Available Artikkeli käsittelee kahta ongelmalliseksi osoittautunutta verbien tempusnimitystä, perfektiä ja imperfektiä. Tarkastelen kirjoituksessani nimitysten välittämää informaatiota, jota vertaan S2-oppikirjoissa aikamuotojen käytöstä annettuun tietoon. Kriittisen katsauksen tavoitteena on osoittaa harhaanjohtavien termien haitta ja hyvän metakielen tarve sekä myös pohtia keinoja virheellisten nimitysten korjaamiseksi. Teoreettisena viitekehyksenä on työolosuhteita, työvälineitä ja työtapoja tutkiva ergonomia. Koska kielen opetus ja opiskelu ovat työtä ja metakieli on työväline, jolla opettaja ja opiskelijat yhdessä työskentelevät, on käytettävän termistön ammatillinen ja käytännönläheinen arviointi tarpeen. DOI: http://dx.doi.org/10.5128/LV22.04

  10. Design features of an automated entry control system

    International Nuclear Information System (INIS)

    Reynolds, D.A.

    1978-01-01

    Features of an entry control system designed to automatically control access to nuclear facilities is described. Control independent of variable human factors is stressed, but security force action is required for assessment and response as a result of an alarm. A design based on a distributed processing capability is utilized. Flexibility and generality are emphasized in an effort to maximize applicability to the entry-control problem faced by nuclear facilities upgrading security as a result of the Safeguards Program

  11. 3D-mallien muokkaus 3D-tulostamista varten CAD-ohjelmilla

    OpenAIRE

    Lehtimäki, Jarmo

    2013-01-01

    Insinöörityössäni käsitellään 3D-mallien tulostamista ja erityisesti 3D-mallien mallintamista niin, että kappaleiden valmistaminen 3D-tulostimella onnistuisi mahdollisimman hyvin. Työ tehtiin Prohoc Oy:lle, joka sijaitsee Vaasassa. 3D-tulostuspalveluun tuli jatkuvasti 3D-malleja, joiden tulostuksessa oli ongelmia. Työssäni tutkin näiden ongelmien syntyä ja tein ohjeita eri 3D-mallinnusohjelmille, joiden tarkoituksena on auttaa tekemään helpommin tulostettavia 3D-malleja. Työhön kuului myös et...

  12. "Arjessa pitää aikalailla olla kaikki palikat itsellä tassuissa" : suomalaisten johtajien työn ja perheen yhteensovittaminen puolisoiden kokemana

    OpenAIRE

    Telanne, Emmi

    2017-01-01

    Emmi Telanne (2017). ”Arjessa pitää aikalailla olla kaikki palikat itsellä tassuissa” - Suomalaisten johtajien työn ja perheen yhteensovittaminen puolisoiden kokemana: Liikuntatieteellinen tiedekunta, Jyväskylän yliopisto, terveyskasvatuksen pro gradu -tutkielma, 52 s., 6 liitettä. Työn ja perheen yhteensovittaminen on tärkeää yksilön, yhteiskunnan, yritysten ja erityisesti perheen näkökulmasta. Tutkimusten mukaan johtajan työn stressi- ja vaatimustekijöillä on havaittu olevan kielteisiä ...

  13. Detection of an inversion in the Ty-2 region between S. lycopersicum and S. habrochaites by a combination of de novo genome assembly and BAC cloning.

    Science.gov (United States)

    Wolters, Anne-Marie A; Caro, Myluska; Dong, Shufang; Finkers, Richard; Gao, Jianchang; Visser, Richard G F; Wang, Xiaoxuan; Du, Yongchen; Bai, Yuling

    2015-10-01

    A chromosomal inversion associated with the tomato Ty - 2 gene for TYLCV resistance is the cause of severe suppression of recombination in a tomato Ty - 2 introgression line. Among tomato and its wild relatives inversions are often observed, which result in suppression of recombination. Such inversions hamper the transfer of important traits from a related species to the crop by introgression breeding. Suppression of recombination was reported for the TYLCV resistance gene, Ty-2, which has been introgressed in cultivated tomato (Solanum lycopersicum) from the wild relative S. habrochaites accession B6013. Ty-2 was mapped to a 300-kb region on the long arm of chromosome 11. The suppression of recombination in the Ty-2 region could be caused by chromosomal rearrangements in S. habrochaites compared with S. lycopersicum. With the aim of visualizing the genome structure of the Ty-2 region, we compared the draft de novo assembly of S. habrochaites accession LYC4 with the sequence of cultivated tomato ('Heinz'). Furthermore, using populations derived from intraspecific crosses of S. habrochaites accessions, the order of markers in the Ty-2 region was studied. Results showed the presence of an inversion of approximately 200 kb in the Ty-2 region when comparing S. lycopersicum and S. habrochaites. By sequencing a BAC clone from the Ty-2 introgression line, one inversion breakpoint was identified. Finally, the obtained results are discussed with respect to introgression breeding and the importance of a priori de novo sequencing of the species involved.

  14. Entry into the electricity market: Uncertainty, competition, and mothballing options

    Energy Technology Data Exchange (ETDEWEB)

    Takashima, Ryuta [Department of Nuclear Engineering and Management, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656 (Japan)], E-mail: takashima@n.t.u-tokyo.ac.jp; Goto, Makoto [Department of Industrial and Management Systems Engineering, Waseda University, 3-4-1 Okubo, Shinjyuku-ku, Tokyo 169-8555 (Japan); Kimura, Hiroshi; Madarame, Haruki [Nuclear Professional School, University of Tokyo, 2-22 Shirane, Shirakata, Tokai-mura, Naka-Gun, Ibaraki-ken 319-1188 (Japan)

    2008-07-15

    The present paper analyzes the entry strategies into the electricity market of two firms that have power plants under price uncertainty and competition. We consider the symmetric and asymmetric two firms, which have either a thermal power plant or a nuclear power plant. The differences between the thermal power plant and the nuclear power plant, such as the cost structure and operational flexibility are modeled. The threshold values of market entry are calculated for each firm with either the thermal power plant or the nuclear power plant as the leader or the follower. We show the dependence of cost structures on entry thresholds of the leader and the follower into the electricity market. For various market and cost conditions, the diagrams of the leader are also shown.

  15. Entry into the electricity market: Uncertainty, competition, and mothballing options

    International Nuclear Information System (INIS)

    Takashima, Ryuta; Goto, Makoto; Kimura, Hiroshi; Madarame, Haruki

    2008-01-01

    The present paper analyzes the entry strategies into the electricity market of two firms that have power plants under price uncertainty and competition. We consider the symmetric and asymmetric two firms, which have either a thermal power plant or a nuclear power plant. The differences between the thermal power plant and the nuclear power plant, such as the cost structure and operational flexibility are modeled. The threshold values of market entry are calculated for each firm with either the thermal power plant or the nuclear power plant as the leader or the follower. We show the dependence of cost structures on entry thresholds of the leader and the follower into the electricity market. For various market and cost conditions, the diagrams of the leader are also shown

  16. 15 N utilization in nitride nuclear fuels for advanced nuclear power reactors and accelerator - driven systems

    International Nuclear Information System (INIS)

    Axente, D.

    2005-01-01

    15 N utilization for nitride nuclear fuels production for nuclear power reactors and accelerator - driven systems is presented. Nitride nuclear fuel is the obvious choice for advanced nuclear reactors and ADS because of its favorable properties: a high melting point, excellent thermal conductivity, high fissile density, lower fission gas release and good radiation tolerance. The application of nitride fuels in nuclear reactors and ADS requires use of 15 N enriched nitrogen to suppress 14 C production due to (n,p) reaction on 14 N. Accelerator - driven system is a recent development merging of accelerator and fission reactor technologies to generate electricity and transmute long - lived radioactive wastes as minor actinides: Np, Am, Cm. A high-energy proton beam hitting a heavy metal target produces neutrons by spallation. The neutrons cause fission in the fuel, but unlike in conventional reactors, the fuel is sub-critical and fission ceases when the accelerator is turned off. Nitride fuel is a promising candidate for transmutation in ADS of minor actinides, which are converted into nitrides with 15 N for that purpose. Tacking into account that the world wide market is about 20 to 40 Kg 15 N annually, the supply of that isotope for nitride fuel production for nuclear power reactors and ADS would therefore demand an increase in production capacity by a factor of 1000. For an industrial plant producing 100 t/y 15 N, using present technology of isotopic exchange in NITROX system, the first separation stage of the cascade would be fed with 10M HNO 3 solution of 600 mc/h flow - rate. If conversion of HNO 3 into NO, NO 2 , at the enriching end of the columns, would be done with gaseous SO 2 , for a production plant of 100 t/y 15 N a consumption of 4 million t SO 2 /y and a production of 70 % H 2 SO 4 waste solution of 4.5 million mc/y are estimated. The reconversion of H 2 SO 4 into SO 2 in order to recycle of SO 2 is a problem to be solved to compensate the cost of SO 2

  17. Evolutionary characterization of Ty3/gypsy-like LTR retrotransposons in the parasitic cestode Echinococcus granulosus.

    Science.gov (United States)

    Bae, Young-An

    2016-11-01

    Cyclophyllidean cestodes including Echinococcus granulosus have a smaller genome and show characteristics such as loss of the gut, a segmented body plan, and accelerated growth rate in hosts compared with other tissue-invading helminths. In an effort to address the molecular mechanism relevant to genome shrinkage, the evolutionary status of long-terminal-repeat (LTR) retrotransposons, which are known as the most potent genomic modulators, was investigated in the E. granulosus draft genome. A majority of the E. granulosus LTR retrotransposons were classified into a novel characteristic clade, named Saci-2, of the Ty3/gypsy family, while the remaining elements belonged to the CsRn1 clade of identical family. Their nucleotide sequences were heavily corrupted by frequent base substitutions and segmental losses. The ceased mobile activity of the major retrotransposons and the following intrinsic DNA loss in their inactive progenies might have contributed to decrease in genome size. Apart from the degenerate copies, a gag gene originating from a CsRn1-like element exhibited substantial evidences suggesting its domestication including a preserved coding profile and transcriptional activity, the presence of syntenic orthologues in cestodes, and selective pressure acting on the gene. To my knowledge, the endogenized gag gene is reported for the first time in invertebrates, though its biological function remains elusive.

  18. Production of 15N for nitride type nuclear fuel

    International Nuclear Information System (INIS)

    Axente, Damian

    2005-01-01

    Full text: Nitride nuclear fuel is the choice for advanced nuclear reactors and ADS, considering its favorable properties as: melting point, excellent thermal conductivity, high fissile density, lower fission gas release and good radiation tolerance. The application of nitride fuels in different nuclear reactors requires use of 15 N enriched nitrogen to suppress 14 C production due to (n,p) reaction on 14 N. Nitride fuel is a promising candidate for transmutation in ADSs of radioactive minor actinides, which are converted into nitrides with 15 N for that purpose. Taking into account that at present the world wide 15 N market is about 20 - 40 Kg 15 N/y, the supply of that isotope for nitride type nuclear fuel, would demand an increase in production capacity by a factor of 1000. For an industrial plant producing 100 t/y 15 N at 99 at. % 15 N concentration, using present technology of 15 N/ 14 N isotopic exchange in Nitrox system, the first separation stage of the cascade would be fed with 10M HNO 3 solution at a 600 m 3 /h flow-rate. If conversion of HNO 3 into NO, NO 2 , at the enriching end of the columns, would be done with gaseous SO 2 , for an industrial plant of 100 t/y 15 N a consumption of 4 million t SO 2 /y and a production of 70 % H 2 SO 4 waste solution of 4.5 million m 3 /y are estimated. The reconversion of H 2 SO 4 into SO 2 in order to recycle SO 2 is a problem to be solved to compensate the cost of sulfur dioxide and to diminish the amount of sulfuric acid waste solution. It should be taken into consideration an important price reduction of 15 N in order to make possible its utilization for industrial production of nitride type nuclear fuel. (authors)

  19. Borehole data package for well 299-W15-41 at single-shell tank waste management Area TX-TY

    International Nuclear Information System (INIS)

    Horton, D.G.; Hodges, F.N.

    2000-01-01

    One new Resource Conservation and Recovery Act (RCRA) groundwater monitoring well was installed at the single-shell tank farm Waste Management Area (WMA) TX-TY during December 1999 and January 2000 in fulfillment of Tri-Party Agreement (Ecology 1996) milestone M-24-43. The well is 299-W15-41 and is located south of the 241-TX tank farm and south of 20th Street in the 200 West Area. A figure shows the locations of all wells in the WMA TX-TY monitoring network. The new well was constructed to the specifications and requirements described in Washington Administrative Code (WAC) 173-160 and WAC 173-303, the groundwater monitoring plan for WMA TX-TY (Caggiano and Goodwin 1991), the assessment plan for WMA TX-TY (Caggiano and Chou 1993), and the description of work for well drilling and installation. This document compiles information on the drilling and construction, well development, pump installation, and sediment testing applicable to well 299-W1 5-41. Appendix A contains the geologist's log, the Well Construction Summary Report, and Well Summary Sheet (as-built diagram) and Appendix B contains borehole geophysical logs. Additional documentation concerning well construction is on file with Bechtel Hanford, Inc., Richland, Washington

  20. Systemic and Terminal Ileum Mucosal Immunity Elicited by Oral Immunization With the Ty21a Typhoid Vaccine in HumansSummary

    Directory of Open Access Journals (Sweden)

    Jayaum S. Booth

    2017-11-01

    Full Text Available Background & Aims: Systemic cellular immunity elicited by the Ty21a oral typhoid vaccine has been extensively characterized. However, very limited data are available in humans regarding mucosal immunity at the site of infection (terminal ileum [TI]. Here we investigated the host immunity elicited by Ty21a immunization on terminal ileum–lamina propria mononuclear cells (LPMC and peripheral blood in volunteers undergoing routine colonoscopy. Methods: We characterized LPMC-T memory (TM subsets and assessed Salmonella enterica serovar Typhi (S Typhi–specific responses by multichromatic flow cytometry. Results: No differences were observed in cell yields and phenotypes in LPMC CD8+-TM subsets following Ty21a immunization. However, Ty21a immunization elicited LPMC CD8+ T cells exhibiting significant S Typhi–specific responses (interferon-γ, tumor necrosis factor-α, interleukin-17A, and/or CD107a in all major TM subsets (T-effector/memory [TEM], T-central/memory, and TEM-CD45RA+, although each TM subset exhibited unique characteristics. We also investigated whether Ty21a immunization elicited S Typhi–specific multifunctional effectors in LPMC CD8+ TEM. We observed that LPMC CD8+ TEM responses were mostly multifunctional, except for those cells exhibiting the characteristics associated with cytotoxic responses. Finally, we compared mucosal with systemic responses and made the important observation that LPMC CD8+ S Typhi–specific responses were unique and distinct from their systemic counterparts. Conclusions: This study provides the first demonstration of S Typhi–specific responses in the human terminal ileum mucosa and provides novel insights into the generation of mucosal immune responses following oral Ty21a immunization. Keywords: Lamina Propria Mononuclear Cells, Multifunctional T Cells, CD8+-T Memory Cells, Typhoid, Vaccines

  1. CCD Photometry of W UMa Type Binary TY UMa

    Directory of Open Access Journals (Sweden)

    Young Woon Kang

    2001-06-01

    Full Text Available We present VRI CCD photometry of W UMa type binary TY UMa. The light curves show that the secondary minimum is deeper than theprimary minimum and the maximum I (0.p25 is 0.m023 brighter than the maximum II (0.p75. The V light curve has beenanalyzed and the photometric solutions have been determined by the method of Wilson & Devinney differential correction. Weadopted the spot model to explain the asymetric light curve.

  2. Productive Entry of Foot-and-Mouth Disease Virus via Macropinocytosis Independent of Phosphatidylinositol 3-Kinase

    Science.gov (United States)

    Han, Shi-Chong; Guo, Hui-Chen; Sun, Shi-Qi; Jin, Ye; Wei, Yan-Quan; Feng, Xia; Yao, Xue-Ping; Cao, Sui-Zhong; Xiang Liu, Ding; Liu, Xiang-Tao

    2016-01-01

    Virus entry is an attractive target for therapeutic intervention. Here, using a combination of electron microscopy, immunofluorescence assay, siRNA interference, specific pharmacological inhibitors, and dominant negative mutation, we demonstrated that the entry of foot-and-mouth disease virus (FMDV) triggered a substantial amount of plasma membrane ruffling. We also found that the internalization of FMDV induced a robust increase in fluid-phase uptake, and virions internalized within macropinosomes colocalized with phase uptake marker dextran. During this stage, the Rac1-Pak1 signaling pathway was activated. After specific inhibition on actin, Na+/H+ exchanger, receptor tyrosine kinase, Rac1, Pak1, myosin II, and protein kinase C, the entry and infection of FMDV significantly decreased. However, inhibition of phosphatidylinositol 3-kinase (PI3K) did not reduce FMDV internalization but increased the viral entry and infection to a certain extent, implying that FMDV entry did not require PI3K activity. Results showed that internalization of FMDV exhibited the main hallmarks of macropinocytosis. Moreover, intracellular trafficking of FMDV involves EEA1/Rab5-positive vesicles. The present study demonstrated macropinocytosis as another endocytic pathway apart from the clathrin-mediated pathway. The findings greatly expand our understanding of the molecular mechanisms of FMDV entry into cells, as well as provide potential insights into the entry mechanisms of other picornaviruses. PMID:26757826

  3. "Tää on ollu niinku toinen koti" : Kankaan päiväkodin lasten vanhempien ja työntekijöiden kokemuksia sisarusryhmästä

    OpenAIRE

    Pulkka, Laura; Halonen, Tiina

    2011-01-01

    Tiivistelmä Opinnäytetyön aiheena on Kankaan päiväkodin sisarusryhmä, jota tutkitaan lasten vanhempien ja työntekijöiden kokemuksien pohjalta. Työssä selvitetään, millainen vaikutus päiväkodin sisarusryhmällä on lapsen kehitykseen ja sisarusten väliseen suhteeseen vanhempien ja päiväkodin työntekijöiden näkökulmasta. Lisäksi tutkitaan, millaisia hyötyjä ja haittoja sisarusryhmässä on. Haastatteluista saadut vastaukset ovat vanhempien ja työntekijöiden subjektiivisia näkemyksiä aiheesta. ...

  4. ITSM-työkalun käyttöönotto konsernissa : Kriittisiin menestystekijöihin pohjautuva kehityshanke

    OpenAIRE

    Pajunen, Eero

    2012-01-01

    Yrityksessä X ollaan ottamassa käyttöön uusi ITSM-työkalu ServiceNow. Toistaiseksi ohjelmistosta on otettu käyttöön Incident Management -osio ja jatkossa tullaan ottamaan käyttöön Problem Management- ja Change Management -osiot. Uuden ITSM-työkalun Incident Management -osion käyttöönotto yrityksen X ICT Segment -tiimissä on ollut odotettua hitaampaa, koska konsernitasolla tapahtuu haastavia muutoksia. Kehityshankkeen tavoitteena on ensimmäisessä käyttöönotossa todettujen asioiden kautta l...

  5. Planetary Mission Entry Vehicles Quick Reference Guide. Version 3.0

    Science.gov (United States)

    Davies, Carol; Arcadi, Marla

    2006-01-01

    This is Version 3.0 of the planetary mission entry vehicle document. Three new missions, Re-entry F, Hayabusa, and ARD have been added to t he previously published edition (Version 2.1). In addition, the Huyge ns mission has been significantly updated and some Apollo data correc ted. Due to the changing nature of planetary vehicles during the desi gn, manufacture and mission phases, and to the variables involved in measurement and computation, please be aware that the data provided h erein cannot be guaranteed. Contact Carol Davies at cdavies@mail.arc. nasa.gov to correct or update the current data, or to suggest other missions.

  6. Henkilöstöjohtamisella vaikutusta työhyvinvointiin : Henkilöstöjohtamisen käsikirja tukena esimiestyön haasteissa

    OpenAIRE

    Viitasaari, Tuomas

    2014-01-01

    Yhteiskunnallisesti katsottuna työhyvinvointi on tärkeässä asemassa. Hyvällä johtamisella voidaan vaikuttaa työhyvinvointiin ja sen parantamiseen. Tässä opinnäytetyössä tutkitaan käytännön henkilöstöjohtamista ja henkilöstöjohtamisen käsikirjan merkitystä esimiestyössä. Opinnäytteeni ensimmäisenä tavoitteena on vastata siihen, mikä on hyvää henkilöstöjohtamista ja miten sitä voidaan tukea. Toinen tavoite on työpaikkani SPR Kontti kierrätystavarataloketjun toimeksiannon mukaisesti tutkia henki...

  7. EntrySat: A 3U CubeStat to study the reentry atmospheric environment

    Science.gov (United States)

    Anthony, Sournac; Raphael, Garcia; David, Mimoun; Jeremie, Chaix

    2016-04-01

    ISAE France Entrysat has for main scientific objective the study of uncontrolled atmospheric re-entry. This project, is developed by ISAE in collaboration with ONERA and University of Toulouse, is funded by CNES, in the overall frame of the QB50 project. This nano-satellite is a 3U Cubesat measuring 34*10*10 cm3, similar to secondary debris produced during the break up of a spacecraft. EntrySat will collect the external and internal temperatures, pressure, heat flux, attitude variations and drag force of the satellite between ≈150 and 90 km before its destruction in the atmosphere, and transmit them during the re-entry using the IRIDIUM satellite network. The result will be compared with the computations of MUSIC/FAST, a new 6-degree of freedom code developed by ONERA to predict the trajectory of space debris. In order to fulfil the scientific objectives, the satellite will acquire 18 re-entry sensors signals, convert them and compress them, thanks to an electronic board developed by ISAE students in cooperation with EREMS. In order to transmit these data every second during the re-entry phase, the satellite will use an IRIDIUM connection. In order to keep a stable enough attitudes during this phase, a simple attitude orbit and control system using magnetotorquers and an inertial measurement unit (IMU) is developed at ISAE by students. A commercial GPS board is also integrated in the satellite into Entry Sat to determine its position and velocity which are necessary during the re-entry phase. This GPS will also be used to synchronize the on-board clock with the real-time UTC data. During the orbital phase (≈2 year) EntrySat measurements will be recorded transmitted through a more classical "UHF/VHF" connection. Preference for presentation: Poster Most suitable session: Author for correspondence: Dr Raphael F. Garcia ISAE 10, ave E. Belin, 31400 Toulouse, France Raphael.GARCIA@isae.fr +33 5 61 33 81 14

  8. Toisen asteen ammatillinen koulutus ja työssäoppiminen

    OpenAIRE

    Vesa, Kalle

    2017-01-01

    ”Niin muuttuu maailma, Eskoni”- Nummisuutarit (Aleksis Kivi, 1864) Toisen asteen ammatillisen koulutuksen reformi on tuloillaan ja uudistuneiden lakien on tarkoitus astua voimaan 1.1.2018. Hallituksen ohjelman mukaan mm. ammatillisen koulutuksen yhteiskunnallista merkitystä vahvistetaan ja toisen asteen koulutusta uudistetaan vastaamaan tulevaisuuden osaamistarpeita. Työpaikalla tapahtuvaa oppimista pyritään lisäämään ja samalla parantamaan sen laatua. Oppimisympäristöjä digitalisoidaan j...

  9. "Parasta oli, että työntekijä vaivautui tulemaan meille asti, monta kertaa." : Kokemuksia ratkaisukeskeisestä neuropsykiatrisesta valmennuksesta

    OpenAIRE

    Isomettä, Jenni

    2012-01-01

    Opinnäytetyön aihe nousi esiin Tampereen kaupungin lasten ja nuorten terveyspalveluiden OSMO-hankkeesta. Työelämätaho oli vakuuttunut tämänkaltaisen työn tarpeellisuudesta. OSMO-lyhenne tulee sanoista osaamisverkostoista monimuotoinen tuki lasten ja nuorten kehitysympäristöihin. Opinnäytetyössä kartoitettiin Tampereen kaupungin OSMO-hankkeen ratkaisukeskeiseen neuropsykiatriseen valmennukseen osallistuneiden kokemuksia valmennuksesta. Opinnäytetyössä selvitettiin, mitä he ovat kokeneet valmen...

  10. Tank 241-TY-101 vapor sampling and analysis tank characterization report

    International Nuclear Information System (INIS)

    Huckaby, J.L.

    1995-01-01

    This report presents the details of the Hanford waste tank characterization study for tank 241-TY-101. The drivers and objectives of the headspace vapor sampling and analysis were in accordance with procedure that were presented in other reports. The vapor and headspace gas samples were collected and analyzed to determine the potential risks to tank farm workers due to fugitive emissions from the tank

  11. Ty1-copia elements reveal diverse insertion sites linked to polymorphisms among flax (Linum usitatissimum L.) accessions.

    Science.gov (United States)

    Galindo-González, Leonardo; Mhiri, Corinne; Grandbastien, Marie-Angèle; Deyholos, Michael K

    2016-12-07

    Initial characterization of the flax genome showed that Ty1-copia retrotransposons are abundant, with several members being recently inserted, and in close association with genes. Recent insertions indicate a potential for ongoing transpositional activity that can create genomic diversity among accessions, cultivars or varieties. The polymorphisms generated constitute a good source of molecular markers that may be associated with phenotype if the insertions alter gene activity. Flax, where accessions are bred mainly for seed nutritional properties or for fibers, constitutes a good model for studying the relationship of transpositional activity with diversification and breeding. In this study, we estimated copy number and used a type of transposon display known as Sequence-Specific Amplification Polymorphisms (SSAPs), to characterize six families of Ty1-copia elements across 14 flax accessions. Polymorphic insertion sites were sequenced to find insertions that could potentially alter gene expression, and a preliminary test was performed with selected genes bearing transposable element (TE) insertions. Quantification of six families of Ty1-copia elements indicated different abundances among TE families and between flax accessions, which suggested diverse transpositional histories. SSAPs showed a high level of polymorphism in most of the evaluated retrotransposon families, with a trend towards higher levels of polymorphism in low-copy number families. Ty1-copia insertion polymorphisms among cultivars allowed a general distinction between oil and fiber types, and between spring and winter types, demonstrating their utility in diversity studies. Characterization of polymorphic insertions revealed an overwhelming association with genes, with insertions disrupting exons, introns or within 1 kb of coding regions. A preliminary test on the potential transcriptional disruption by TEs of four selected genes evaluated in three different tissues, showed one case of significant

  12. Clustering pesticides according to their molecular properties and their impacts by considering additional ecotoxicological parameters in the TyPol method

    Science.gov (United States)

    Traore, Harouna; Crouzet, Olivier; Mamy, Laure; Sireyjol, Christine; Rossard, Virginie; Servien, Remy; Latrille, Eric; Benoit, Pierre

    2017-04-01

    The understanding of the fate of pesticides and their environmental impacts largely relies on their molecular properties. We recently developed 'TyPol' (Typology of Pollutants), a clustering method based on statistical analyses combining several environmental endpoints (i.e. environmental parameters such as sorption coefficient, degradation half-life) and one ecotoxicological one (bioconcentration factor), and structural molecular descriptors (number of atoms in the molecule, molecular surface, dipole moment, energy of orbitals…). TyPol has been conceived on the available knowledge on QSAR of a wide diversity of organic compounds (Mamy et al., 2015). This approach also allows to focus on transformation products present in different clusters and to infer possible changes in environmental fate consecutively to different degradation processes (Servien et al., 2014; Benoit et al., 2016). The initial version of TyPol did not include any ecotoxicological parameters except the bioconcentration factor (BCF), which informs more on the transfer along the trophic chain rather than on the effects on non-target organisms. The objective was to implement the TyPol database with a data set of ecotoxicological data concerning pesticides and several aquatic and terrestrial organisms, in order to test the possibility to extend TyPol to ecotoxicological effects on various organisms. The data analysis (available literature and databases) revealed that relevant ecotoxicological endpoints for terrestrial organisms such as soil microorganisms and macroinvertebrates are lacking compared to aquatic organisms. We have added seven parameters for acute (EC50, LC50) and chronic (NOEC) toxicological effects for the following organisms: Daphnia, Algae, Lemna and Earthworm. In this new configuration, TyPol was used to classify about 45 pesticides in different behavioural and ecotoxicity clusters. The clustering results were analyzed to reveals relationships between molecular descriptors

  13. DATA-ENTRY-3: some observations and pragmatics of a structured design. [In FORTRAN for PDP-11/10

    Energy Technology Data Exchange (ETDEWEB)

    Sparks, D.

    1977-08-01

    The FORTRAN program DATA-ENTRY-3 was developed from the COBOL program DATA-ENTRY-1, which solves a large class of elementary data-capture, data-formating, and data-editing problems of managerial accounting. Most of the work involved finding methods to make DATA-ENTRY-3, which is written for a small-machine environment (PDP-11/10 under the RT-11 operating system), logically equivalent to DATA-ENTRY-1, which is written for a large-machine environment (CDC 6600 under a time-sharing operating system). This report explains how structured programing helped, and briefly describes the function of each subroutine.

  14. Ukrainian Nuclear Data Centre Progress Report, 2010/11. Summary of Nuclear Data Studies by Staff of the Ukrainian Nuclear Data Centre

    International Nuclear Information System (INIS)

    Gritzay, O.

    2011-01-01

    Full text: Ukrainian Nuclear Data Centre (UkrNDC) is subdivision within the Neutron Physics Department at the Institute for Nuclear Research of the National Academy of Sciences of Ukraine. UkrNDC has 5 permanent researchers. During year under review three members of the staff were involved in experimental neutron data measurements at the Kyiv research reactor. Compilation: We continue collection and compilation of experimental neutron, charged particle and photonuclear data. Number of the EXFOR's entries sent to the NDS IAEA by UkrNDC are the following. New: for neutron data - 1 entries (32231); for charged particle data - 4 entries (D5074, D5075, D5077, D5078); for photonuclear data - 4 entries (G4030 - G4033). Improved: for neutron data - 5 entries (32217, 32219, 32222 - 32224); for charged particle data - 15 entries (D5001, D5002, D5023, D5030, D5039- D5042, D5051, D5066 - D5068, D5070, D5071, D5073); for photonuclear data - 2 entries (G4011, G4018). We realize review of compilation scope in home journals: ''Nuclear Physics and Atomic Energy''; ''Ukrainian Journal of Physics''; ''Problems of Atomic Science and Technology'', Series ''Nuclear Physics Investigations''. Collaboration: We continue our collaboration with the Nuclear Physics Department of the Taras Shevchenko National University of Kyiv. The teaching course 'Nuclear Data for Science and Technology and modern computer codes for nuclear data processing' (36 hours) has been lectured in 2010-2011 for the fifth-course students of NPD KNU. This course includes the following items: ENDF/B libraries, EXROR system, ENSDF library, the use of the PREPRO code in work with the ENDF libraries, the introduction to NJOY code system, the Network of Nuclear Reaction Data Centers and the use of the on-line services; The teaching courses 'Neutron Physics' (72 hours) and 'Neutron Physics at the Kyiv Research Reactor' (experimental work, 36 hours) are lectured in 2010-2011 for fourth-year students of NPD KNU; The teaching

  15. Jäsen 360° -datan vaikutukset Ylöjärven seurakunnan työn kehittämisprosessissa

    OpenAIRE

    Korppoo-Seppänen, Riikka

    2017-01-01

    Tämän opinnäytetyön tarkoituksena oli selvittää, onko Ylöjärven seurakunnan työn kehittämisprosessissa hyödynnetty Jäsen 360° -dataa ja miten se on vaikuttanut työn tekemiseen ja koko seurakunnan toiminnan suunnitteluun ja ohjaukseen. Tarkoituksena oli myös selvittää minkälaisissa tilanteissa ja miten kehittämisprosessi on muuttanut henkilöstön toimintatapoja sekä onko Jäsen 360° -datan avulla muutettu toiminnan suuntaamista. Tutkimuksen avulla selvitettiin Jäsen 360° -datan käytön rajoitteit...

  16. Identification and characterization of REC66, a Ty1-copia-like retrotransposon in the genome of red flower of Mirabilis jalapa L.

    Directory of Open Access Journals (Sweden)

    Shunri Jiang

    2017-01-01

    Full Text Available Mirabilis jalapa Lis the most commonly grown ornamental species of Mirabilis and is available in a range of brilliant colors. However, genetic research on Mirabilis jalapa Lis limited. Using fluorescent differential display (FDD screening, we report the identification of a novel Ty1-copia-like retrotransposon in the genome of the red flower of Mirabilis jalapa L, and we named it REC66based on its sequence homology to the GAG protein from Ty1-copiaretrotransposon. Using degenerate primers based on the DNA sequence of REC66, a total of fourteen different variants in reverse transcriptase (RT sequence were recovered from the genomic DNA. These RT sequences show a high degree of heterogeneity characterized mainly by deletion mutation; they can be divided into three subfamilies, of which the majority encode defective RT. This is the first report of a Ty1-copiaretrotransposon in Mirabilis jalapa L. The finding could be helpful for the development of new molecular markers for genetic studies, particularly on the origin and evolutionary relationships of M. jalapa L, and the study of Ty1-copiaretrotransposons and plant genome evolution in the genus Mirabilisor family Nyctaginaceae.

  17. NOPS 2010–2012 –hanke, Nuoren työ- ja toimintakyvyn edistäminen ammattiopistoissa : Ammattiopiston opiskelijan terveysosaaminen terveystapahtumien jälkeen : Terveystapahtumien toteutuminen ammattiopistoissa keväällä 2011

    OpenAIRE

    Javanainen, Jonna; Leino, Maria; Kahri, Vesa

    2012-01-01

    Tämä opinnäytetyö on osa NOPS 2010–2012 -hankkeesta (Nuoren työ- ja toimintakyvyn edistäminen ammattiopistoissa). NOPS 2010–2012 -hankkeen tarkoituksena on luoda nuoren työ- ja toimintakykyä edistävä toimintamalli, jossa ammattikorkeakoulun opiskelijat kytketään osaksi ammattiopistojen terveyttä edistävää työtä. Hankkeessa kehitetään myös sellaisia terveyden edistämisen menetelmiä, jotka soveltuvat ammattiopistossa opiskelevien nuorten työ- ja toimintakyvyn edistämiseen. Hankkeessa tehdä...

  18. Results of Phase I groundwater quality assessment for single-shell tank waste management areas T and TX-TY at the Hanford Site

    International Nuclear Information System (INIS)

    Hodges, F.N.

    1998-01-01

    Pacific Northwest National Laboratory (PNNL) conducted a Phase I, Resource Conservation and Recovery Act of 1976 (RCRA) groundwater quality assessment for the Richland Field Office of the U.S. Department of Energy (DOE-RL) under the requirements of the Federal Facility Compliance Agreement. The purpose of the investigation was to determine if the Single-Shell Tank Waste Management Areas (WMAs) T and TX-TY have impacted groundwater quality. Waste Management Areas T and TX-TY, located in the northern part of the 200 West Area of the Hanford Site, contain the 241-T, 241-TX, and 241-TY tank farms and ancillary waste systems. These two units are regulated under RCRA interim-status regulations (under 40 CFR 265.93) and were placed in assessment groundwater monitoring because of elevated specific conductance in downgradient wells. Anomalous concentrations of technetium-99, chromium, nitrate, iodine-129, and cobalt-60 also were observed in some downgradient wells. Phase I assessment, allowed under 40 CFR 265, provides the owner-operator of a facility with the opportunity to show that the observed contamination has a source other than the regulated unit. For this Phase I assessment, PNNL evaluated available information on groundwater chemistry and past waste management practices in the vicinity of WMAs T and TX-TY. Background contaminant concentrations in the vicinity of WMAs T and TX-TY are the result of several overlapping contaminant plumes resulting from past-practice waste disposal operations. This background has been used as baseline for determining potential WMA impacts on groundwater

  19. TY-0048 in radiotherapy for carcinoma of the head and neck

    International Nuclear Information System (INIS)

    Mizukoshi, Takanori; Yamashita, Tetsuro; Fukuda, Hiroshi; Tomita, Kinai

    1981-01-01

    TY-0048, a mucolytic agent, was administered to 3 patients (2 with gingiva tumor and 1 with tongue cancer) who underwent radiotherapy, and the results were evaluated. The drug was given to the 2 patients with gingiva tumor simultaneously with the initiation of radiotherapy. After receiving 1,500 to 2,000 rad of irradiation, the patients had difficulty in coughing up sputa due to increased viscosity. The third patient with tongue cancer who received large field irradiation took the drug after he had apparently increased viscosity of the sputa and difficulty in coughing up to sputa. The drug demonstrated to be effective in decreasing viscosity of the sputa and making it easy for this patient to cough up the sputa. This drug, in a preparation of syrup, is readily used for stomatitis and xerostomia. (Ueda, j.)

  20. 15 CFR 301.3 - Application for duty-free entry of scientific instruments.

    Science.gov (United States)

    2010-01-01

    ... 15 Commerce and Foreign Trade 2 2010-01-01 2010-01-01 false Application for duty-free entry of scientific instruments. 301.3 Section 301.3 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) INTERNATIONAL TRADE ADMINISTRATION, DEPARTMENT OF COMMERCE MISCELLANEOUS...

  1. Small Nuclear Technology and Market Entry

    International Nuclear Information System (INIS)

    Stewart, J S; Schock, R N; Brown, N W; Smith, C F

    2002-01-01

    An overview of energy-system projections into the new century leads to the conclusion that nuclear power will play a significant role. How significant a role will be determined by the marketplace. Within the range of nuclear-power technologies available, small nuclear-power plants of innovative design appear to fit the needs of a number of developing nations and states. Under similar financing options used by the airline industry and others, the capital requirement barrier that puts the nuclear industry at a disadvantage in deregulated markets could be reduced. These plants have the potential advantage of modularity, are proliferation-resistant, incorporate passive safety features, minimize waste, and could be cost-competitive with fossil-fuel plants

  2. Diaspora, a large family of Ty3-gypsy retrotransposons in Glycine max, is an envelope-less member of an endogenous plant retrovirus lineage.

    Science.gov (United States)

    Yano, Sho T; Panbehi, Bahman; Das, Arpita; Laten, Howard M

    2005-05-05

    The chromosomes of higher plants are littered with retrotransposons that, in many cases, constitute as much as 80% of plant genomes. Long terminal repeat retrotransposons have been especially successful colonizers of the chromosomes of higher plants and examinations of their function, evolution, and dispersal are essential to understanding the evolution of eukaryotic genomes. In soybean, several families of retrotransposons have been identified, including at least two that, by virtue of the presence of an envelope-like gene, may constitute endogenous retroviruses. However, most elements are highly degenerate and are often sequestered in regions of the genome that sequencing projects initially shun. In addition, finding potentially functional copies from genomic DNA is rare. This study provides a mechanism to surmount these issues to generate a consensus sequence that can then be functionally and phylogenetically evaluated. Diaspora is a multicopy member of the Ty3-gypsy-like family of LTR retrotransposons and comprises at least 0.5% of the soybean genome. Although the Diaspora family is highly degenerate, and with the exception of this report, is not represented in the Genbank nr database, a full-length consensus sequence was generated from short overlapping sequences using a combination of experimental and in silico methods. Diaspora is 11,737 bp in length and contains a single 1892-codon ORF that encodes a gag-pol polyprotein. Phylogenetic analysis indicates that it is closely related to Athila and Calypso retroelements from Arabidopsis and soybean, respectively. These in turn form the framework of an endogenous retrovirus lineage whose members possess an envelope-like gene. Diaspora appears to lack any trace of this coding region. A combination of empirical sequencing and retrieval of unannotated Genome Survey Sequence database entries was successfully used to construct a full-length representative of the Diaspora family in Glycine max. Diaspora is presently the

  3. Role of a nuclear localization signal on the minor capsid Proteins VP2 and VP3 in BKPyV nuclear entry

    Energy Technology Data Exchange (ETDEWEB)

    Bennett, Shauna M. [Cellular and Molecular Biology Program University of Michigan 1150W Medical Center Dr 5724 Medical Science Bldg II Ann Arbor, MI 48109 (United States); Zhao, Linbo [Doctoral Program in Cancer Biology Program University of Michigan 1150W Medical Center Dr 5724 Medical Science Bldg II Ann Arbor, MI 48109 (United States); Bosard, Catherine [Department of Microbiology and Immunology University of Michigan 1150W Medical Center Dr 5724 Medical Science Bldg II Ann Arbor, MI 48109 (United States); Imperiale, Michael J., E-mail: imperial@umich.edu [Cellular and Molecular Biology Program University of Michigan 1150W Medical Center Dr 5724 Medical Science Bldg II Ann Arbor, MI 48109 (United States); Doctoral Program in Cancer Biology Program University of Michigan 1150W Medical Center Dr 5724 Medical Science Bldg II Ann Arbor, MI 48109 (United States); Department of Microbiology and Immunology University of Michigan 1150W Medical Center Dr 5724 Medical Science Bldg II Ann Arbor, MI 48109 (United States)

    2015-01-01

    BK Polyomavirus (BKPyV) is a ubiquitous nonenveloped human virus that can cause severe disease in immunocompromised populations. After internalization into renal proximal tubule epithelial cells, BKPyV traffics through the ER and enters the cytosol. However, it is unclear how the virus enters the nucleus. In this study, we elucidate a role for the nuclear localization signal located on the minor capsid proteins VP2 and VP3 during infection. Site-directed mutagenesis of a single lysine in the basic region of the C-terminus of the minor capsid proteins abrogated their nuclear localization, and the analogous genomic mutation reduced infectivity. Additionally, through use of the inhibitor ivermectin and knockdown of importin β1, we found that the importin α/β pathway is involved during infection. Overall these data are the first to show the significance of the NLS of the BKPyV minor capsid proteins during infection in a natural host cell. - Highlights: • Polyomaviruses must deliver their genome to the nucleus to replicate. • The minor capsid proteins have a well-conserved nuclear localization signal. • Mutation of this NLS diminishes, but does not completely inhibit, infection.

  4. Role of a nuclear localization signal on the minor capsid Proteins VP2 and VP3 in BKPyV nuclear entry

    International Nuclear Information System (INIS)

    Bennett, Shauna M.; Zhao, Linbo; Bosard, Catherine; Imperiale, Michael J.

    2015-01-01

    BK Polyomavirus (BKPyV) is a ubiquitous nonenveloped human virus that can cause severe disease in immunocompromised populations. After internalization into renal proximal tubule epithelial cells, BKPyV traffics through the ER and enters the cytosol. However, it is unclear how the virus enters the nucleus. In this study, we elucidate a role for the nuclear localization signal located on the minor capsid proteins VP2 and VP3 during infection. Site-directed mutagenesis of a single lysine in the basic region of the C-terminus of the minor capsid proteins abrogated their nuclear localization, and the analogous genomic mutation reduced infectivity. Additionally, through use of the inhibitor ivermectin and knockdown of importin β1, we found that the importin α/β pathway is involved during infection. Overall these data are the first to show the significance of the NLS of the BKPyV minor capsid proteins during infection in a natural host cell. - Highlights: • Polyomaviruses must deliver their genome to the nucleus to replicate. • The minor capsid proteins have a well-conserved nuclear localization signal. • Mutation of this NLS diminishes, but does not completely inhibit, infection

  5. Tutkimustiedon käyttö röntgenhoitajan työssä osana näyttöön perustuvaa toimintaa

    OpenAIRE

    Vesikukka, E. (Emilia)

    2015-01-01

    Tiivistelmä Tämän tutkimuksen tarkoituksena oli kuvailla röntgenhoitajien tutkimustiedon käyttöä työssään röntgenhoitajien arvioimana. Tutkimuksen tavoitteena oli lisätä tietoisuutta tutkimustiedon käyttötottumuksista sekä tutkimustiedon käyttöön vaikuttavista tekijöistä, joiden avulla voitaisiin pyrkiä edistämään näyttöön perustuvaa radiografiaa osaksi työkulttuuria. ...

  6. 3D-mallinnuksen oppimistehtävät

    OpenAIRE

    Lepistö, Timo

    2016-01-01

    Työn tarkoituksena on selvittää miten luodaan selkeitä ja yksinkertaisia tehtäviä 3D-mallinnuksen opiskeluun. Näiden asioiden perusteella tehdään kolme erilaista oppimistehtävää 3D-mallinnuksen opiskeluun. Taustana on oma osaaminen 3D-mallinnuksesta, joten tavoitteena on parantaa omaa 3D-mallinnuksen osaamista. Työssä käsitellään yleisesti 3D-mallinnuksen tekniikoita, joita käytetään oppimistehtävissä sekä työssä käytettävää 3ds Max Design-mallinnusohjelmaa. Näiden lisäksi tutustutaan oppi...

  7. Monimuoto-opiskelijoiden ajanhallinta ja jaksaminen : kokemuksia perheen, työn ja opiskelun yhteensovittamisesta

    OpenAIRE

    Ranne, Satu-Maaret; Salminen, Piia

    2011-01-01

    Ranne Satu-Maaret ja Salminen Piia. Monimuoto-opiskelijoiden ajanhallinta ja jaksaminen - kuinka sovittaa yhteen perhe, työ ja opiskelu. Diakonia-ammattikorkeakoulun monimuoto-opiskelijoiden kokemuksia ajanhallinnasta ja jaksamisesta. Pori, kevät 2011, 34 s. Diakonia-ammattikorkeakoulu, Diak Länsi, Pori. Hoitotyön koulutusohjelma. Sairaanhoitaja (AMK). Opinnäytetyön tarkoituksena oli tutkia ammattikorkeakoulussa monimuotoisesti opiskelevien opiskelijoiden ajanhallintaa ja jaksamista: ...

  8. Moniosaaminen ja työnkierto - Kuinka saada henkilökunta innostumaan osaamisen lisäämisestä ja työnkierrosta

    OpenAIRE

    Murto, Maria

    2017-01-01

    Tämän opinnäytetyön tarkoituksena on löytää keinoja ylläpitää ja innostaa henkilökuntaa Pirkanmaan käräjäoikeudessa moniosaamiseen ja tehtävänkiertoon. Ajatus tähän tehtävään oli kypsynyt mielessäni jo pitkään ja itse tutkimuksen aihe syntyi yhdessä laamannin kanssa keskusteltaessa. Digitalisoituminen on yleistynyt ja sen myötä osaamisen kehittäminen ja sen ylläpitäminen ovat nousseet oleelliseen asemaan työelämässä. Tietojärjestelmät kehittyvät ja sen myötä joudutaan ottamaan uusia toim...

  9. Evaluation of training programs and entry-level qualifications for nuclear-power-plant control-room personnel based on the systems approach to training

    International Nuclear Information System (INIS)

    Haas, P.M.; Selby, D.L.; Hanley, M.J.; Mercer, R.T.

    1983-09-01

    This report summarizes results of research sponsored by the US Nuclear Regulatory Commission (NRC) Office of Nuclear Regulatory Research to initiate the use of the Systems Approach to Training in the evaluation of training programs and entry level qualifications for nuclear power plant (NPP) personnel. Variables (performance shaping factors) of potential importance to personnel selection and training are identified, and research to more rigorously define an operationally useful taxonomy of those variables is recommended. A high-level model of the Systems Approach to Training for use in the nuclear industry, which could serve as a model for NRC evaluation of industry programs, is presented. The model is consistent with current publically stated NRC policy, with the approach being followed by the Institute for Nuclear Power Operations, and with current training technology. Checklists to be used by NRC evaluators to assess training programs for NPP control-room personnel are proposed which are based on this model

  10. Evaluation of training programs and entry-level qualifications for nuclear-power-plant control-room personnel based on the systems approach to training

    Energy Technology Data Exchange (ETDEWEB)

    Haas, P M; Selby, D L; Hanley, M J; Mercer, R T

    1983-09-01

    This report summarizes results of research sponsored by the US Nuclear Regulatory Commission (NRC) Office of Nuclear Regulatory Research to initiate the use of the Systems Approach to Training in the evaluation of training programs and entry level qualifications for nuclear power plant (NPP) personnel. Variables (performance shaping factors) of potential importance to personnel selection and training are identified, and research to more rigorously define an operationally useful taxonomy of those variables is recommended. A high-level model of the Systems Approach to Training for use in the nuclear industry, which could serve as a model for NRC evaluation of industry programs, is presented. The model is consistent with current publically stated NRC policy, with the approach being followed by the Institute for Nuclear Power Operations, and with current training technology. Checklists to be used by NRC evaluators to assess training programs for NPP control-room personnel are proposed which are based on this model.

  11. Digitaalinen opas Osuuspankin työntekijöille tietoisuuden lisäämiseksi blockchain-teknologiasta ja sen hyödyistä

    OpenAIRE

    Kangasmäki, Meeri

    2016-01-01

    Opinnäytetyö on laadittu toimeksiantona Osuuspankille. Opinnäytetyön toimeksiannon tavoitteena oli tuottaa opas blockchain-teknologiasta ja sen hyödyistä Osuuspankin työntekijöille. Oppaan tarkoituksena on lisätä ymmärrystä blockchain-teknologian toiminnasta ja sen käyttömahdollisuuksista. Blockchain-teknologia tulee muuttamaan finanssialaa lähitulevaisuudessa ja siksi siitä on tärkeä lisätä tietoisuutta alalla työskentelevien keskuudessa. Opinnäytetyössä esitellään blockchain-teknologian...

  12. Energy Information Data Base: corporate author entries

    International Nuclear Information System (INIS)

    1978-06-01

    The DOE Energy Information Data Base has been created and is maintained by the DOE Technical Information Center. One of the controls for information entered into the base is the standardized name of the corporate entity or the corporate author. The purpose of this list of authorized or standardized corporate entries is to provide a means for the consistent citing of the names of organizations in bibliographic records. It also serves as a guide for users who retrieve information from a bibliographic data base and who want to locate information originating in particular organizations. This authority is a combination of entries established by the Technical Information Center and the International Atomic Energy Agency's International Nuclear Information System (INIS). The format calls, in general, for the name of the organization represented by the literature being cataloged to be cited as follows: the largest element, the place, the smallest element, e.g., Brigham Young Univ., Provo, Utah (USA), Dept. of Chemical Engineering. Code numbers are assigned to each entry to provide manipulation by computer. Cross references are used to reflect name changes and invalid entries

  13. Hormone-dependent nuclear export of estradiol receptor and DNA synthesis in breast cancer cells

    Science.gov (United States)

    Lombardi, Maria; Castoria, Gabriella; Migliaccio, Antimo; Barone, Maria Vittoria; Di Stasio, Rosina; Ciociola, Alessandra; Bottero, Daniela; Yamaguchi, Hiroshi; Appella, Ettore; Auricchio, Ferdinando

    2008-01-01

    In breast cancer cells, cytoplasmic localization of the estradiol receptor α (ERα) regulates estradiol-dependent S phase entry. We identified a nuclear export sequence (NES) in ERα and show that its export is dependent on both estradiol-mediated phosphatidylinositol-3-kinase (PI3K)/AKT activation and chromosome region maintenance 1 (CRM1). A Tat peptide containing the ERα NES disrupts ERα–CRM1 interaction and prevents nuclear export of ERα- and estradiol-induced DNA synthesis. NES-ERα mutants do not exit the nucleus and inhibit estradiol-induced S phase entry; ERα-dependent transcription is normal. ERα is associated with Forkhead proteins in the nucleus, and estradiol stimulates nuclear exit of both proteins. ERα knockdown or ERα NES mutations prevent ERα and Forkhead nuclear export. A mutant of forkhead in rhabdomyosarcoma (FKHR), which cannot be phosphorylated by estradiol-activated AKT, does not associate with ERα and is trapped in the nucleus, blocking S phase entry. In conclusion, estradiol-induced AKT-dependent phosphorylation of FKHR drives its association with ERα, thereby triggering complex export from the nucleus necessary for initiation of DNA synthesis and S phase entry. PMID:18644889

  14. Force Triggers YAP Nuclear Entry by Regulating Transport across Nuclear Pores.

    Science.gov (United States)

    Elosegui-Artola, Alberto; Andreu, Ion; Beedle, Amy E M; Lezamiz, Ainhoa; Uroz, Marina; Kosmalska, Anita J; Oria, Roger; Kechagia, Jenny Z; Rico-Lastres, Palma; Le Roux, Anabel-Lise; Shanahan, Catherine M; Trepat, Xavier; Navajas, Daniel; Garcia-Manyes, Sergi; Roca-Cusachs, Pere

    2017-11-30

    YAP is a mechanosensitive transcriptional activator with a critical role in cancer, regeneration, and organ size control. Here, we show that force applied to the nucleus directly drives YAP nuclear translocation by decreasing the mechanical restriction of nuclear pores to molecular transport. Exposure to a stiff environment leads cells to establish a mechanical connection between the nucleus and the cytoskeleton, allowing forces exerted through focal adhesions to reach the nucleus. Force transmission then leads to nuclear flattening, which stretches nuclear pores, reduces their mechanical resistance to molecular transport, and increases YAP nuclear import. The restriction to transport is further regulated by the mechanical stability of the transported protein, which determines both active nuclear transport of YAP and passive transport of small proteins. Our results unveil a mechanosensing mechanism mediated directly by nuclear pores, demonstrated for YAP but with potential general applicability in transcriptional regulation. Copyright © 2017 Elsevier Inc. All rights reserved.

  15. Proposed nuclear weapons nonproliferation policy concerning foreign research reactor spent nuclear fuel: Appendix A, environmental justice analysis. Volume 2

    International Nuclear Information System (INIS)

    1995-03-01

    This is Appendix A to a draft Environmental Impact Statement on a Proposed Nuclear Weapons Nonproliferation Policy Concerning Foreign Research Reactor Spent Nuclear Fuel. This appendix addresses environmental justice for the acceptance of foreign research reactor spent nuclear fuel containing uranium enriched in the United States. Analyses of environmental justice concerns are provided in three areas: (1) potential ports of entry, (2) potential transportation routes from candidate ports of entry to interim management sites, and (3) areas surrounding potential interim management sites. These analyses lead to the conclusion that the alternatives analyzed in this Environmental Impact Statement (EIS) would result in no disproportionate adverse effects on minority populations or low-income communities surrounding the candidate ports, transport routes, or interim management sites

  16. The nuclear protein Sam68 is cleaved by the FMDV 3C protease redistributing Sam68 to the cytoplasm during FMDV infection of host cells

    International Nuclear Information System (INIS)

    Lawrence, Paul; Schafer, Elizabeth A.; Rieder, Elizabeth

    2012-01-01

    Picornavirus infection can lead to disruption of nuclear pore traffic, shut-off of cell translation machinery, and cleavage of proteins involved in cellular signal transduction and the innate response to infection. Here, we demonstrated that the FMDV 3C pro induced the cleavage of nuclear RNA-binding protein Sam68 C-terminus containing the nuclear localization sequence (NLS). Consequently, it stimulated the redistribution of Sam68 to the cytoplasm. The siRNA knockdown of Sam68 resulted in a 1000-fold reduction in viral titers, which prompted us to study the effect of Sam68 on FMDV post-entry events. Interestingly, Sam68 interacts with the internal ribosomal entry site within the 5′ non-translated region of the FMDV genome, and Sam68 knockdown decreased FMDV IRES-driven activity in vitro suggesting that it could modulate translation of the viral genome. The results uncover a novel role for Sam68 in the context of picornaviruses and the proteolysis of a new cellular target of the FMDV 3C pro .

  17. Medical student satisfaction, coping and burnout in direct-entry versus graduate-entry programmes.

    Science.gov (United States)

    DeWitt, Dawn; Canny, Benedict J; Nitzberg, Michael; Choudri, Jennifer; Porter, Sarah

    2016-06-01

    There is ongoing debate regarding the optimal length of medical training, with concern about the cost of prolonged training. Two simultaneous tracks currently exist in Australia: direct entry from high school and graduate entry for students with a bachelor degree. Medical schools are switching to graduate entry based on maturity, academic preparedness and career-choice surety. We tested the assumption that graduate entry is better by exploring student preferences, coping, burnout, empathy and alcohol use. From a potential pool of 2188 participants, enrolled at five Australian medical schools, a convenience sample of 688 (31%) first and second year students completed a survey in the middle of the academic year. Participants answered questions about demographics, satisfaction and coping and completed three validated instruments. Over 90% of students preferred their own entry-type, though more graduate-entry students were satisfied with their programme (82.4% versus 65.3%, p students in self-reported coping or in the proportion of students meeting criteria for burnout (50.7% versus 51.2%). Direct-entry students rated significantly higher for empathy (concern, p = 0.022; personal distress, p = 0.031). Graduate-entry students reported significantly more alcohol use and hazardous drinking (30.0% versus 22.8%; p = 0.017). Our multi-institution data confirm that students are generally satisfied with their choice of entry pathway and do not confirm significant psychosocial benefits of graduate entry. Overall, our data suggest that direct-entry students cope with the workload and psychosocial challenges of medical school, in the first 2 years, as well as graduate-entry students. Burnout and alcohol use should be addressed in both pathways. Despite studies showing similar academic outcomes, and higher total costs, more programmes in Australia are becoming graduate entry. Further research on non-cognitive issues and outcomes is needed so that universities, government

  18. 3D-tulostusmenetelmien käyttö auton osien valmistuksessa

    OpenAIRE

    Löfgren, Jani

    2015-01-01

    Tavoitteena tässä insinöörityössä on tarkastella 3D-tulostusmenetelmiä ja niiden mahdollisuuksia valmistaa auton osia pienerissä tai kustomoituina. Työssä keskitytään kertomaan 3D-tulostusmenetelmistä ja materiaaleista, jotka soveltuvat parhaiten auton osien valmistukseen. Työn alussa käydään läpi 3D-tulostustekniikkaa ja eri valmistusmenetelmiä. Tarkasteluun on valittu neljä parhaiten auton osien valmistukseen soveltuvaksi arvioitua tulostusmenetelmää. Tämän jälkeen työssä tarkastellaan ...

  19. SURFACE GEOPHYSICAL EXPLORATION OF TX-TY TANK FARMS AT THE HANFORD SITE: RESULTS OF BACKGROUND CHARACTERIZATION WITH GROUND PENETRATING RADAR

    International Nuclear Information System (INIS)

    MYERS DA; CUBBAGE R; BRAUCHLA R; O'BRIEN G

    2008-01-01

    Ground penetrating radar surveys of the TX and TY tank farms were performed to identify existing infrastructure in the near surface environment. These surveys were designed to provide background information supporting Surface-to-Surface and Well-to-Well resistivity surveys of Waste Management Area TX-TY. The objective of the preliminary investigation was to collect background characterization information with GPR to understand the spatial distribution of metallic objects that could potentially interfere with the results from high resolution resistivity(trademark) surveys. The results of the background characterization confirm the existence of documented infrastructure, as well as highlight locations of possible additional undocumented subsurface metallic objects

  20. Preliminary tank characterization report for single-shell tank 241-TY-101: best-basis inventory

    International Nuclear Information System (INIS)

    Lambert, S.L.

    1997-01-01

    An effort is underway to provide waste inventory estimates that will serve as standard characterization source terms for the various waste management activities. As part of this effort, an evaluation of available information for single-shell tank 241-TY-101 was performed, and a best-basis inventory was established. This work follows the methodology that was established by the standard inventory task

  1. Prelimainary tank characterization report for single-shell tank 241-TY-103 : Best-Basis inventory

    International Nuclear Information System (INIS)

    Hendrickson, D.W.

    1997-01-01

    An effort is underway to provide waste inventory estimates that will serve as standard characterization source terms for the various waste management activities. As part of this effort, an evaluation of available information for single-shell tank 241'-TY-103 was performed, and a best-basis inventory was established. This work follows the methodology that was established by the standard inventory task

  2. Sairaanhoitajan työhön perehdytys lastensuojelulaitoksessa : Sairaanhoitajan perehdytyssuunnitelma Helsingin Diakonissalaitoksen intensiivihoidon osastoille

    OpenAIRE

    Pitkälä, Pia

    2013-01-01

    Pitkälä, Pia. ”Sairaanhoitajan työhön perehdytys lastensuojelulaitoksessa. Sairaanhoitajan perehdytyssuunnitelma Helsingin Diakonissalaitoksen intensiivihoidon osastoille.” Helsinki, syksy 2013, s.56, 2 liitettä. Diakonia-ammattikorkeakoulu, hoitotyön koulutusohjelma, sairaanhoitaja (AMK). Opinnäytetyön tarkoituksena oli laatia sairaanhoitajien perehdytyssuunnitelma Diakonissalaitoksen lapsi- ja perhetyön palvelualueen osana toimiville intensiivihoidon osastoille. Tavoitteena oli kehittää...

  3. SURFACE GEOPHYSICAL EXPLORATION OF TX AND TY TANK FARMS AT THE HANFORD SITE RESULTS OF BACKGROUND CHARACTERIZATION WITH MAGNETICS AND ELECTROMAGNETICS

    International Nuclear Information System (INIS)

    MYERS DA; RUCKER D; LEBITT M; CUBBAGE B; HENDERSON

    2008-01-01

    This report documents the results of preliminary surface geophysical exploration activities performed between September and October 2007 at the waste management areas surrounding the TX and TY tank farms. The TX-TY tank farms are located in the 200 West Area of the US Department of Energy's Hanford Site in Washington State. The objective of the preliminary investigation was to acquire background characterization information using magnetic gradiometry (Mag) and electromagnetic induction (EM) methods to understand the spatial distribution of buried metallic objects that could potentially interfere with the results of a subsequently completed high resolution resistivity survey

  4. CRM-järjestelmän suunnittelu ja käyttöönotto myynnin strategiseksi työkaluksi : case: Stala Oy

    OpenAIRE

    Pirkkalainen, Markus

    2013-01-01

    Tämän opinnäytetyön aiheena on asiakkuudenhallinta- eli Customer Relationship Management(CRM)-järjestelmän suunnittelu ja käyttöönotto myynnin strategiseksi työkaluksi. Opinnäytetyön teoriaosuudessa tutkitaan asiakkuudenhallintaa, CRM-järjestelmiä ja CRM-järjestelmien suunnitteluun sekä käyttöönottoon liittyviä haasteita. Työn empiirinen osuus koostuu CRM-järjestelmän käyttöoppaasta kohdeyritys Stala Oy:n myyntitiimille. Tutkimusongelma vastaa kysymykseen, miksi yrityksen tulisi panostaa a...

  5. 8 CFR 289.3 - Recording the entry of certain American Indians born in Canada.

    Science.gov (United States)

    2010-01-01

    ... Indians born in Canada. 289.3 Section 289.3 Aliens and Nationality DEPARTMENT OF HOMELAND SECURITY IMMIGRATION REGULATIONS AMERICAN INDIANS BORN IN CANADA § 289.3 Recording the entry of certain American Indians born in Canada. The lawful admission for permanent residence of an American Indian born in Canada...

  6. "Yhdessä hyvää" hyvin toimiva työyhteisö ravintola Kiesassa

    OpenAIRE

    Jaaranen, Minka

    2014-01-01

    Ravintola Kiesa kuuluu osana Pohjois-Karjalan Ammattiopisto, Joensuu, Palvelujen toimintaan. Oppilaitoksen tavoitteena on kouluttaa opiskelijoita, joilla on hyvät tiedot ja taidot kokin ja tarjoilijan työhön. Ravintola Kiesa oppimisympäristönä mahdollistaa taitojen harjoittelun. Kiesassa on toimivat uudet tilat ja laitteet sekä osaava ja kouluttautuva henkilökunta. Haasteen Kiesan toiminnalle luovat jatkuvasti vaihtuvat opiskelijaryhmät opettajineen. Ratkaisua haetaan kysymyksiin: kuinka yht...

  7. Triglyceride-glucose index (TyG index) in comparison with fasting plasma glucose improved diabetes prediction in patients with normal fasting glucose: The Vascular-Metabolic CUN cohort.

    Science.gov (United States)

    Navarro-González, David; Sánchez-Íñigo, Laura; Pastrana-Delgado, Juan; Fernández-Montero, Alejandro; Martinez, J Alfredo

    2016-05-01

    We evaluated the potential role of the triglyceride-glucose index (TyG index) as a predictor of diabetes in a White European cohort, and compared it to fasting plasma glucose (FPG) and triglycerides. 4820 patients of the Vascular-Metabolic CUN cohort (VMCUN cohort) were examined and followed up for 8.84years (±4.39). We performed a Cox proportional hazard ratio with repeated-measures analyses to assess the risk of developing type 2 diabetes across quartiles of FPG, triglycerides and the TyG index (ln[fasting triglycerides (mg/dl)×fasting plasma glucose (mg/dl)/2]), and plotted a receiver operating characteristics (ROC) curve for discrimination. There were 332 incident cases of type 2 diabetes involving 43,197.32person-years of follow-up. We observed a progressively increased risk of diabetes in subjects with TyG index levels of 8.31 or more. Among those with normal fasting glucose at baseline, index in the fourth quartile were 6.87 times more likely to develop diabetes (95% CI, 2.76-16.85; P for trendindex, 0.66 (0.60-0.72) for FPG and 0.71 (0.65-0.77) for TG, in subjects with normal fasting glucose (p=0.017). Our data suggest that the TyG index is useful for the early identification of individuals at risk of type 2 diabetes. The TyG index seems to be a better predictor than FPG or triglycerides of the potential development of type 2 diabetes in normoglycemic patients. Copyright © 2016 Elsevier Inc. All rights reserved.

  8. 3D-mallien suunnittelu ja toteutus peliympäristöön

    OpenAIRE

    Mattila, Nina

    2016-01-01

    Tämän opinnäytetyön tarkoituksena oli tutkia peliympäristön suunnittelua ja toteutusta 3D-mallintajan näkökulmasta. Työssä keskityttiin tutkimaan mallintamiseen liittyviä teknisiä rajoitteita ja pelimalleihin soveltuvia mallinnustekniikoita. Tutkimusosuudessa tehtiin katsaus 3D-mallintajan rooliin pelinkehitysprojektissa ja tutkittiin 3D-grafiikan käyttötarkoituksia peleissä. Työssä esiteltiin myös käytetyimpiä 3D-mallinnusohjelmia sekä muita mallintamistyössä käytettäviä työkaluja. Tutki...

  9. Y-sukupolven ajatukset työelämästä: Motivointi ja sitouttaminen

    OpenAIRE

    Nikkilä, Henni

    2016-01-01

    Opinnäytetyö suoritettiin toimeksiantona finanssi-ja vakuutusalalla toimivalle organisaatiolle. Kohdeorganisaatiolla on tarve selvittää miten Y-sukupolvi (1980–1990-luvulla syntyneet henkilöt) motivoituvat ja miten heidät saadaan sitoutettua työpaikkaan. Y-sukupolvi eli niin sanottu nouseva sukupolvi eroaa aiemmista sukupolvista siinä, että he ovat tavallisesti teknologian kasvatteja, sosiaalisesti verkostoituneita ja tasavertaisten oikeuksien puolestapuhujia. Y-sukupolvi arvostaa mielenk...

  10. The phosphatidylinositol-3-phosphate 5-kinase inhibitor apilimod blocks filoviral entry and infection.

    Directory of Open Access Journals (Sweden)

    Elizabeth A Nelson

    2017-04-01

    Full Text Available Phosphatidylinositol-3-phosphate 5-kinase (PIKfyve is a lipid kinase involved in endosome maturation that emerged from a haploid genetic screen as being required for Ebola virus (EBOV infection. Here we analyzed the effects of apilimod, a PIKfyve inhibitor that was reported to be well tolerated in humans in phase 2 clinical trials, for its effects on entry and infection of EBOV and Marburg virus (MARV. We first found that apilimod blocks infections by EBOV and MARV in Huh 7, Vero E6 and primary human macrophage cells, with notable potency in the macrophages (IC50, 10 nM. We next observed that similar doses of apilimod block EBOV-glycoprotein-virus like particle (VLP entry and transcription-replication competent VLP infection, suggesting that the primary mode of action of apilimod is as an entry inhibitor, preventing release of the viral genome into the cytoplasm to initiate replication. After providing evidence that the anti-EBOV action of apilimod is via PIKfyve, we showed that it blocks trafficking of EBOV VLPs to endolysosomes containing Niemann-Pick C1 (NPC1, the intracellular receptor for EBOV. Concurrently apilimod caused VLPs to accumulate in early endosome antigen 1-positive endosomes. We did not detect any effects of apilimod on bulk endosome acidification, on the activity of cathepsins B and L, or on cholesterol export from endolysosomes. Hence by antagonizing PIKfyve, apilimod appears to block EBOV trafficking to its site of fusion and entry into the cytoplasm. Given the drug's observed anti-filoviral activity, relatively unexplored mechanism of entry inhibition, and reported tolerability in humans, we propose that apilimod be further explored as part of a therapeutic regimen to treat filoviral infections.

  11. Atmospheric Entry Studies for Venus Missions: 45 Sphere-Cone Rigid Aeroshells and Ballistic Entries

    Science.gov (United States)

    Prabhu, Dinesh K.; Spilker, Thomas R.; Allen, Gary A., Jr.; Hwang, Helen H.; Cappuccio, Gelsomina; Moses, Robert W.

    2013-01-01

    The present study considers direct ballistic entries into the atmosphere of Venus using a 45deg sphere-cone rigid aeroshell, a legacy shape that has been used successfully in the past in the Pioneer Venus Multiprobe Mission. For a number of entry mass and heatshield diameter combinations (i.e., various ballistic coefficients) and entry velocities, the trajectory space in terms of entry flight path angles between skip out and -30deg is explored with a 3DoF trajectory code, TRAJ. From these trajectories, the viable entry flight path angle space is determined through the use of mechanical and thermal performance limits on the thermal protection material and science payload; the thermal protection material of choice is entry-grade carbon phenolic, for which a material thermal response model is available. For mechanical performance, a 200 g limit is placed on the peak deceleration load experienced by the science instruments, and 10 bar is assumed as the pressure limit for entry-grade carbon-phenolic material. For thermal performance, inflection points in the total heat load distribution are used as cut off criteria. Analysis of the results shows the existence of a range of critical ballistic coefficients beyond which the steepest possible entries are determined by the pressure limit of the material rather than the deceleration load limit.

  12. Nuclear Computerized Library for Assessing Reactor Reliability (NUCLARR): Guide to data processing and revision: Part 2, Human error probability data entry and revision procedures

    International Nuclear Information System (INIS)

    Gilmore, W.E.; Gertman, D.I.; Gilbert, B.G.; Reece, W.J.

    1988-11-01

    The Nuclear Computerized Library for Assessing Reactor Reliability (NUCLARR) is an automated data base management system for processing and storing human error probability (HEP) and hardware component failure data (HCFD). The NUCLARR system software resides on an IBM (or compatible) personal micro-computer. Users can perform data base searches to furnish HEP estimates and HCFD rates. In this manner, the NUCLARR system can be used to support a variety of risk assessment activities. This volume, Volume 3 of a 5-volume series, presents the procedures used to process HEP and HCFD for entry in NUCLARR and describes how to modify the existing NUCLARR taxonomy in order to add either equipment types or action verbs. Volume 3 also specifies the various roles of the administrative staff on assignment to the NUCLARR Clearinghouse who are tasked with maintaining the data base, dealing with user requests, and processing NUCLARR data. 5 refs., 34 figs., 3 tabs

  13. Expanding the role of 3-O sulfated heparan sulfate in herpes simplex virus type-1 entry

    International Nuclear Information System (INIS)

    O'Donnell, Christopher D.; Kovacs, Maria; Akhtar, Jihan; Valyi-Nagy, Tibor; Shukla, Deepak

    2010-01-01

    Heparan sulfate (HS) proteoglycans are commonly exploited by multiple viruses for initial attachment to host cells. Herpes simplex virus-1 (HSV-1) is unique because it can use HS for both attachment and penetration, provided specific binding sites for HSV-1 envelope glycoprotein gD are present. The interaction with gD is mediated by specific HS moieties or 3-O sulfated HS (3-OS HS), which are generated by all but one of the seven isoforms of 3-O sulfotransferases (3-OSTs). Here we demonstrate that several common experimental cell lines express unique sets of 3-OST isoforms. While the isoforms 3-OST-3, -5 and -6 were most commonly expressed, isoforms 3-OST-2 and -4 were undetectable in the cell lines examined. Since most cell lines expressed multiple 3-OST isoforms, we addressed the significance of 3-OS HS in HSV-1 entry by down-regulating 2-O-sulfation, a prerequisite for 3-OS HS formation, by knocking down 2-OST expression by RNA interference (RNAi). 2-OST knockdown was verified by reverse-transcriptase PCR and Western blot analysis, while 3-OS HS knockdown was verified by immunofluorescence. Cells showed a significant decrease in viral entry, suggesting an important role for 3-OS HS. Implicating 3-OS HS further, cells knocked down for 2-OST expression also demonstrated decreased cell-cell fusion when cocultivated with effector cells transfected with HSV-1 glycoproteins. Our findings suggest that 3-OS HS may play an important role in HSV-1 entry into many different cell lines.

  14. Quick look report, Entry 3: Three Mile Island Unit 2, October 16, 1980

    International Nuclear Information System (INIS)

    1981-07-01

    This report summarizes tasks performed during the third entry at Three Mile Island Unit 2. During the entry into containment, which was made on October 16, 1980, additional beta, gamma, and neutron surveys were performed to supplement data obtained on previous entries. In addition, several maintenance tasks were completed including testing the operation of both equipment hatch doors, replacing a loose parts monitor system pre-amplifier, and removing a source range monitor. The five-man entry team accomplished these tasks in about 1-1/2 hours

  15. Silencing of dedicator of cytokinesis (DOCK180) obliterates pregnancy by interfering with decidualization due to blockage of nuclear entry of autoimmune regulator (AIRE).

    Science.gov (United States)

    Mohan, Jasna Jagan; Narayan, Prashanth; Padmanabhan, Renjini Ambika; Joseph, Selin; Kumar, Pradeep G; Laloraya, Malini

    2018-03-08

    Dedicator of cytokinesis (DOCK 180) involved in cytoskeletal reorganization is primarily a cytosolic molecule. It is recently shown to be nuclear in HeLa cells but its nuclear function is not known. The spatiotemporal distribution of DOCK180 in uterus was studied in uterine cytoplasmic and nuclear compartments during the "window of implantation." The functional significance of nuclear DOCK180 was explored by homology modeling, co-immunoprecipitation assays, and mass spectrometric analysis. Dock180's role in early pregnancy was ascertained by Dock 180 silencing and subsequent quantitative real-time PCR and Western blotting analysis. Our study shows a nuclear DOCK180 in the uterus during "window of implantation." Estrogen and progesterone mediate expression and nuclear translocation of DOCK180. The nuclear function of DOCK180 is attributed to its ability to import autoimmune regulator (AIRE) into the nucleus. Silencing of Dock180 inhibited AIRE nuclear shuttling which influenced its downstream targets, thereby affecting decidualization with AIRE and HOXA-10 as the major players as well as lack of implantation site formation due to impact on angiogenesis-associated genes. DOCK180 has an indispensable role in pregnancy establishment as knocking down Dock180 abrogates pregnancy by a consolidated impact on decidualization and angiogenesis by regulating AIRE nuclear entry. © 2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  16. Structure-activity relationships of 3-O-β-chacotriosyl oleanic acid derivatives as entry inhibitors for highly pathogenic H5N1 influenza virus.

    Science.gov (United States)

    Li, Sumei; Jia, Xiuhua; Shen, Xintian; Wei, Zhuwen; Jiang, Zhiyan; Liao, Yixian; Guo, Yiming; Zheng, Xiaojun; Zhong, Guohua; Song, Gaopeng

    2017-08-15

    Highly pathogenic H5N1 virus (H5N1) entry is a key target for the development of novel anti-influenza agents with new mechanisms of action. In our continuing efforts to identify novel potential anti-H5N1 entry inhibitors, a series of 3-O-β-chacotriosyl oleanolic acid analogs have been designed, synthesized and evaluated as H5N1 entry inhibitors based on two small molecule inhibitors 1 and 2 previously discovered by us. The anti-H5N1 entry activities were determined based on HA/HIV and VSVG/HIV entry assays. Compound 15 displayed the most promising anti-H5N1 entry activities with average IC 50 values of 4.05μM and good selective index (22.9). Detailed structure-activity relationships (SARs) studies suggested that either the introduction of an additional oxo group to position 11 at OA or alteration of the C-3 configuration of OA from 3β- to 3α-forms can significantly enhance the selective index while maintaining their antiviral activities in vitro. Molecular simulation analysis confirmed that the compounds exert their inhibitory activity through binding tightly to hemagglutinin (HA2) protein near the fusion peptide and prevent virus entry. Copyright © 2017 Elsevier Ltd. All rights reserved.

  17. Accounting systems for special nuclear material control. Technical report

    International Nuclear Information System (INIS)

    Korstad, P.A.

    1980-05-01

    Nuclear material accounting systems were examined and compared to financial double-entry accounting systems. Effective nuclear material accounting systems have been designed using the principles of double-entry financial accounting. The modified double-entry systems presently employed are acceptable if they provide adequate control over the recording and summarizing of transactions. Strong internal controls, based on principles of financial accounting, can help protect nuclear materials and produce accurate, reliable accounting data. An electronic data processing system can more accurately maintain large volumes of data and provide management with more current, reliable information

  18. "...Aikaa kohdata rauhassa lapsi..." : Päivähoidossa työskentelevien kokemuksia vertaisryhmässään sivuun jäävistä lapsista

    OpenAIRE

    Kaiponen, Jaana; Koskela, Jenna

    2009-01-01

    Kaiponen, Jaana & Koskela, Jenna. ”…Aikaa kohdata rauhassa lapsi…” - Päivähoidossa työskentelevien kokemuksia vertaisryhmässään sivuun jäävistä lapsista. Oulu, syksy 2009, 66 s., 2 liitettä. Diakonia-ammattikorkeakoulu, Diak Pohjoinen Oulu. Sosiaalialan koulutusohjelma, Diakonisen sosiaalityön suuntautumisvaihtoehto, sosionomi (AMK) + diakonin virkakelpoisuus. Opinnäytetyön tarkoituksena oli kuvata päivähoidon työntekijöiden kokemuksia vertaisryhmässään sivuun jäävistä lapsista. Tavoittee...

  19. Arkea, ammattitaitoa ja yhteistyötä : työntekijöiden kokemuksia käytöshäiriöisten nuorten hoidosta koulukodissa

    OpenAIRE

    Haunia, Henna

    2011-01-01

    TIIVISTELMÄ Haunia, Henna. Arkea, ammattitaitoa ja yhteistyötä : työntekijöiden kokemuksia käytöshäiriöisten nuorten hoidosta koulukodissa. Helsinki, syksy 2011. Diakonia-ammattikorkeakoulu, Diak Etelä, Helsinki. Sosiaalialan koulutusohjelma, sosionomi (YAMK). Tämän opinnäytetyön tarkoituksena oli tutkia koulukodissa tapahtuvaan käytöshäiriöisten nuorten hoitoon liittyviä työmenetelmiä ja kehittämisen tarpeita. Tässä laadullisessa tutkimuksessa tutkimusmenetelmänä oli toimintatutkimus....

  20. Guessing lexicon entries using finite-state methods

    OpenAIRE

    Koskenniemi, Kimmo Matti

    2018-01-01

    A practical method for interactive guessing of LEXC lexicon entries is presented. The method is based on describing groups of similarly inflected words using regular expressions. The patterns are compiled into a finite-state transducer (FST) which maps any word form into the possible LEXC lexicon entries which could generate it. The same FST can be used (1) for converting conventional headword lists into LEXC entries, (2) for interactive guessing of entries, (3) for corpus-assisted interactiv...

  1. Comparative study of four immortalized human brain capillary endothelial cell lines, hCMEC/D3, hBMEC, TY10, and BB19, and optimization of culture conditions, for an in vitro blood–brain barrier model for drug permeability studies

    OpenAIRE

    Eigenmann, Daniela E; Xue, Gongda; Kim, Kwang S; Moses, Ashlee V; Hamburger, Matthias; Oufir, Mouhssin

    2013-01-01

    Background: Reliable human in vitro blood brain barrier (BBB) models suitable for high throughput screening are urgently needed in early drug discovery and development for assessing the ability of promising bioactive compounds to overcome the BBB. To establish an improved human in vitro BBB model we compared four currently available and well characterized immortalized human brain capillary endothelial cell lines hCMEC/D3 hBMEC TY10 and BB19 with respect to barrier tightness and paracellular p...

  2. The peace and nuclear war dictionary

    International Nuclear Information System (INIS)

    Ali, S.R.

    1989-01-01

    The Peace and Nuclear War Dictionary is organized so that entries and supplementary data can be located easily and quickly. Items are arranged alphabetically throughout, rather than grouped into chapters. When doubtful about how to locate an entry, consult the general index. Page numbers for terms appear in the index in heavy black type; subsidiary concepts discussed within entries can be found in the index, identified by page numbers in regular type. For study purposes, numerous entries have also been subsumed under major topical headings in the index, affording the reader access to broad classes of related information. The reader can also fully explore a topic by employing the extensive cross-references included in all entries. Many entries can be found as subsidiary terms, but in each case the concept is related to the main entry. The author has adopted the format of this book to provide the reader a variety of useful applications. These include its use as a dictionary and ready reference guide to the global language of peace and nuclear war; a study guide for introductory courses in Nuclear War and Peace of International Relations, or for any specialized course in the area; a supplement to a textbook or a group of paperback monographs adopted for use in these courses; a source of review material for the political science major enrolled in advanced courses; and a social science aid for use in business, education, government, policy sciences, and journalism

  3. Työtapojen integrointi varastotyöhön oppaan avulla. Case: Nilson Group

    OpenAIRE

    Siitonen, Arja

    2013-01-01

    Opinnäytetyön tarkoitus oli kehittää nykyistä toimintaa arvioiden Nilson Groupille integroitu varastotyön vaiheita kuvaava opas. Tavoitteena oli helpottaa työntekijöiden varastotyötä, esimerkiksi kenkien hakua, ja täten parantaa asiakaspalvelua. Opinnäytetyö toteutettiin yhdessä Nilson Groupin kanssa. Nilson Groupiin kuuluvat Suomessa Nilson Shoes, Din Sko, Skopunkten, Radical Sports Shop in Shop ja muutama franchising-pohjainen Ecco Shop in Shop. Tutkimusongelmaksi muodostui varaston käyttö ...

  4. Role of the vaccinia virus O3 protein in cell entry can be fulfilled by its Sequence flexible transmembrane domain

    Energy Technology Data Exchange (ETDEWEB)

    Satheshkumar, P.S.; Chavre, James; Moss, Bernard, E-mail: bmoss@nih.gov

    2013-09-15

    The vaccinia virus O3 protein, a component of the entry–fusion complex, is encoded by all chordopoxviruses. We constructed truncation mutants and demonstrated that the transmembrane domain, which comprises two-thirds of this 35 amino acid protein, is necessary and sufficient for interaction with the entry–fusion complex and function in cell entry. Nevertheless, neither single amino acid substitutions nor alanine scanning mutagenesis revealed essential amino acids within the transmembrane domain. Moreover, replication-competent mutant viruses were generated by randomization of 10 amino acids of the transmembrane domain. Of eight unique viruses, two contained only two amino acids in common with wild type and the remainder contained one or none within the randomized sequence. Although these mutant viruses formed normal size plaques, the entry–fusion complex did not co-purify with the mutant O3 proteins suggesting a less stable interaction. Thus, despite low specific sequence requirements, the transmembrane domain is sufficient for function in entry. - Highlights: • The 35 amino acid O3 protein is required for efficient vaccinia virus entry. • The transmembrane domain of O3 is necessary and sufficient for entry. • Mutagenesis demonstrated extreme sequence flexibility compatible with function.

  5. "Tää on, elämä on" : Epätyypillisinä aikoina työskentelevien yksinhuoltajaäitien vanhemmuuden voimavarat ja kuormittavat tekijät

    OpenAIRE

    Brunou, Miia

    2015-01-01

    Opinnäytetyön tarkoituksena oli tutkia epätyypillisinä aikoina työskentelevien yksinhuoltajaäitien kokemuksia vanhemmuuden voimavaroista ja kuormittavista tekijöistä. Tavoitteena oli selvittää ja kuvata äitien kokemuksia vanhemmuudesta ja erityisesti mitä epätyypillisinä aikoina tehtävä työ merkitsee arjen ja vuorovaikutuksen sujumisessa. Laadullinen opinnäytetyö on ollut osa Jyväskylän ammattikorkeakoulun, Jyväskylän yliopiston ja Terveyden ja hyvinvoinnin laitokset Perheet 24/7 – hankett...

  6. Cutting through - open entries require proper support

    Energy Technology Data Exchange (ETDEWEB)

    Peng, S.S. [West Virginia University, Morgantown, WV (USA). Mining Engineering Dept.

    2000-06-01

    The paper explains the applications of open entries in mining and different roof techniques to relieve longwall abutment pressures. The primary support for the open (or cut-through) entries and recovery rooms are normally similar to other development entries in the same mines. The supplementary supports installed can be divided into the following three types: complete backfill of open entries, supplemental roof and/or rib-bolt reinforcement only - no standing support and rows of standing support with or without supplemental roof and/or rib-bolt reinforcement. 3 figs.

  7. Herpes simplex virus type 2 glycoprotein H interacts with integrin αvβ3 to facilitate viral entry and calcium signaling in human genital tract epithelial cells.

    Science.gov (United States)

    Cheshenko, Natalia; Trepanier, Janie B; González, Pablo A; Eugenin, Eliseo A; Jacobs, William R; Herold, Betsy C

    2014-09-01

    Herpes simplex virus (HSV) entry requires multiple interactions at the cell surface and activation of a complex calcium signaling cascade. Previous studies demonstrated that integrins participate in this process, but their precise role has not been determined. These studies were designed to test the hypothesis that integrin αvβ3 signaling promotes the release of intracellular calcium (Ca2+) stores and contributes to viral entry and cell-to-cell spread. Transfection of cells with small interfering RNA (siRNA) targeting integrin αvβ3, but not other integrin subunits, or treatment with cilengitide, an Arg-Gly-Asp (RGD) mimetic, impaired HSV-induced Ca2+ release, viral entry, plaque formation, and cell-to-cell spread of HSV-1 and HSV-2 in human cervical and primary genital tract epithelial cells. Coimmunoprecipitation studies and proximity ligation assays indicated that integrin αvβ3 interacts with glycoprotein H (gH). An HSV-2 gH-null virus was engineered to further assess the role of gH in the virus-induced signaling cascade. The gH-2-null virus bound to cells and activated Akt to induce a small Ca2+ response at the plasma membrane, but it failed to trigger the release of cytoplasmic Ca2+ stores and was impaired for entry and cell-to-cell spread. Silencing of integrin αvβ3 and deletion of gH prevented phosphorylation of focal adhesion kinase (FAK) and the transport of viral capsids to the nuclear pore. Together, these findings demonstrate that integrin signaling is activated downstream of virus-induced Akt signaling and facilitates viral entry through interactions with gH by activating the release of intracellular Ca2+ and FAK phosphorylation. These findings suggest a new target for HSV treatment and suppression. Herpes simplex viruses are the leading cause of genital disease worldwide, the most common infection associated with neonatal encephalitis, and a major cofactor for HIV acquisition and transmission. There is no effective vaccine. These

  8. Education and training requirements of nuclear power plant personnel

    International Nuclear Information System (INIS)

    Donato, R.; Perlas, C.A.; Conti, E.

    1978-01-01

    This bibliography was compiled by the Scientific Library staff to help in the intensified training program being undertaken by the Philippine Atomic Energy Commission (PAEC) for the nuclear power plant personnel of the Philippines' first nuclear power reactor. This bibliography covers the period 1955 - 1976 of the Nuclear Science Abstracts and is composed of 281 entries. Arrangement of these entries is by broad subject category

  9. A novel nuclear combined power and cooling system integrating high temperature gas-cooled reactor with ammonia–water cycle

    International Nuclear Information System (INIS)

    Luo, Chending; Zhao, Fuqiang; Zhang, Na

    2014-01-01

    Highlights: • We propose a novel nuclear ammonia–water power and cooling cogeneration system. • The high temperature reactor is inherently safe, with exhaust heat fully recovered. • The thermal performances are improved compared with nuclear combined cycle. • The base case attains an energy efficiency of 69.9% and exergy efficiency of 72.5%. • Energy conservation and emission reduction are achieved in this cogeneration way. - Abstract: A nuclear ammonia–water power and refrigeration cogeneration system (NAPR) has been proposed and analyzed in this paper. It consists of a closed high temperature gas-cooled reactor (HTGR) topping Brayton cycle and a modified ammonia water power/refrigeration combined bottoming cycle (APR). The HTGR is an inherently safe reactor, and thus could be stable, flexible and suitable for various energy supply situation, and its exhaust heat is fully recovered by the mixture of ammonia and water in the bottoming cycle. To reduce exergy losses and enhance outputs, the ammonia concentrations of the bottoming cycle working fluid are optimized in both power and refrigeration processes. With the HTGR of 200 MW thermal capacity and 900 °C/70 bar reactor-core-outlet helium, the system achieves 88.8 MW net electrical output and 9.27 MW refrigeration capacity, and also attains an energy efficiency of 69.9% and exergy efficiency of 72.5%, which are higher by 5.3%-points and 2.6%-points as compared with the nuclear combined cycle (NCC, like a conventional gas/steam power-only combined cycle while the topping cycle is a closed HTGR Brayton cycle) with the same nuclear energy input. Compared with conventional separate power and refrigeration generation systems, the fossil fuel saving (based on CH 4 ) and CO 2 emission reduction of base-case NAPR could reach ∼9.66 × 10 4 t/y and ∼26.6 × 10 4 t/y, respectively. The system integration accomplishes the safe and high-efficiency utilization of nuclear energy by power and refrigeration

  10. A role for 3-O-sulfotransferase isoform-4 in assisting HSV-1 entry and spread

    International Nuclear Information System (INIS)

    Tiwari, Vaibhav; O'Donnell, Christopher D.; Oh, Myung-Jin; Valyi-Nagy, Tibor; Shukla, Deepak

    2005-01-01

    Many heparan sulfate (HS) 3-O-sulfotransferase (3-OST) isoforms generate cellular receptors for herpes simplex virus type-1 (HSV-1) glycoprotein D (gD). Interestingly, the ability of 3-OST-4 to mediate HSV-1 entry and cell-to-cell fusion has not been determined, although it is predominantly expressed in the brain, a primary target of HSV-1 infections. We report that expression of 3-OST-4 can render Chinese hamster ovary K1 (CHO-K1) cells susceptible to entry of wild-type and a mutant (Rid1) strain of HSV-1. Evidence for generation of gD receptors by 3-OST-4 was suggested by gD-mediated interference assay and the ability of 3-OST-4 expressing CHO-K1 cells to preferentially bind HSV-1 gD, which could be reversed by prior treatment of cells with HS lyases (heparinases-II/III). In addition, 3-OST-4 expressing CHO-K1 cells acquired the ability to fuse with cells-expressing HSV-1 glycoproteins. Demonstrating specificity, the cell fusion was inhibited by soluble 3-O-sulfated forms of HS, but not unmodified HS. Taken together our results suggest a role of 3-OST-4 in HSV-1 pathogenesis

  11. Using a Human Challenge Model of Infection to Measure Vaccine Efficacy: A Randomised, Controlled Trial Comparing the Typhoid Vaccines M01ZH09 with Placebo and Ty21a.

    Science.gov (United States)

    Darton, Thomas C; Jones, Claire; Blohmke, Christoph J; Waddington, Claire S; Zhou, Liqing; Peters, Anna; Haworth, Kathryn; Sie, Rebecca; Green, Christopher A; Jeppesen, Catherine A; Moore, Maria; Thompson, Ben A V; John, Tessa; Kingsley, Robert A; Yu, Ly-Mee; Voysey, Merryn; Hindle, Zoe; Lockhart, Stephen; Sztein, Marcelo B; Dougan, Gordon; Angus, Brian; Levine, Myron M; Pollard, Andrew J

    2016-08-01

    Typhoid persists as a major cause of global morbidity. While several licensed vaccines to prevent typhoid are available, they are of only moderate efficacy and unsuitable for use in children less than two years of age. Development of new efficacious vaccines is complicated by the human host-restriction of Salmonella enterica serovar Typhi (S. Typhi) and lack of clear correlates of protection. In this study, we aimed to evaluate the protective efficacy of a single dose of the oral vaccine candidate, M01ZH09, in susceptible volunteers by direct typhoid challenge. We performed a randomised, double-blind, placebo-controlled trial in healthy adult participants at a single centre in Oxford (UK). Participants were allocated to receive one dose of double-blinded M01ZH09 or placebo or 3-doses of open-label Ty21a. Twenty-eight days after vaccination, participants were challenged with 104CFU S. Typhi Quailes strain. The efficacy of M01ZH09 compared with placebo (primary outcome) was assessed as the percentage of participants reaching pre-defined endpoints constituting typhoid diagnosis (fever and/or bacteraemia) during the 14 days after challenge. Ninety-nine participants were randomised to receive M01ZH09 (n = 33), placebo (n = 33) or 3-doses of Ty21a (n = 33). After challenge, typhoid was diagnosed in 18/31 (58.1% [95% CI 39.1 to 75.5]) M01ZH09, 20/30 (66.7% [47.2 to 87.2]) placebo, and 13/30 (43.3% [25.5 to 62.6]) Ty21a vaccine recipients. Vaccine efficacy (VE) for one dose of M01ZH09 was 13% [95% CI -29 to 41] and 35% [-5 to 60] for 3-doses of Ty21a. Retrospective multivariable analyses demonstrated that pre-existing anti-Vi antibody significantly reduced susceptibility to infection after challenge; a 1 log increase in anti-Vi IgG resulting in a 71% decrease in the hazard ratio of typhoid diagnosis ([95% CI 30 to 88%], p = 0.006) during the 14 day challenge period. Limitations to the study included the requirement to limit the challenge period prior to treatment to 2

  12. Rakennusten 3d-mallinnusmenetelmät

    OpenAIRE

    Gangsö, Tanja

    2006-01-01

    Tässä opinnäytetyössä käsitellään rakennuksen 3D-mallinnusmenetelmiä. Pääasiassa perehdytäänn rakennusten tuotemalleihin sekä rakennusmallinnukseen visuaalisena projektina. Työssä pyritään tuomaan esille kuinka 3D-malleja voidaan rakennusten yhteydessä monipuolisesti hyödyntää. Keskitytään rakennusten mallinnusmenetelmiin, joita vasta suunnitellaan tai joita ei ole enää olemassa. Työn tavoitteena on esittää rakennusten CAD-mallien sekä visuaalisten mallien ominaisuuksia sekä eroavaisuu...

  13. Interaction of KSHV with Host Cell Surface Receptors and Cell Entry

    Directory of Open Access Journals (Sweden)

    Mohanan Valiya Veettil

    2014-10-01

    Full Text Available Virus entry is a complex process characterized by a sequence of events. Since the discovery of KSHV in 1994, tremendous progress has been made in our understanding of KSHV entry into its in vitro target cells. KSHV entry is a complex multistep process involving viral envelope glycoproteins and several cell surface molecules that is utilized by KSHV for its attachment and entry. KSHV has a broad cell tropism and the attachment and receptor engagement on target cells have an important role in determining the cell type-specific mode of entry. KSHV utilizes heparan sulfate, integrins and EphrinA2 molecules as receptors which results in the activation of host cell pre-existing signal pathways that facilitate the subsequent cascade of events resulting in the rapid entry of virus particles, trafficking towards the nucleus followed by viral and host gene expression. KSHV enters human fibroblast cells by dynamin dependant clathrin mediated endocytosis and by dynamin independent macropinocytosis in dermal endothelial cells. Once internalized into endosomes, fusion of the viral envelope with the endosomal membranes in an acidification dependent manner results in the release of capsids which subsequently reaches the nuclear pore vicinity leading to the delivery of viral DNA into the nucleus. In this review, we discuss the principal mechanisms that enable KSHV to interact with the host cell surface receptors as well as the mechanisms that are required to modulate cell signaling machinery for a successful entry.

  14. Nuclear power plant personnel entry level qualifications and training

    International Nuclear Information System (INIS)

    Jorgensen, C.C.; Haas, P.M.; Selby, D.L.; Lowry, J.C.

    1983-01-01

    This paper summarizes the early results and current status of a research program at ORNL which is intended to provide the methods and technical basis for NRC to initiate the use of the Systems Approach to Training (SAT) in the evaluation of training programs and entry level qualifications for NPP control room personnel. The program is an outgrowth of previous studies of simulator hardware and simulator training requirements under the Safety Related Operator Actions Program which recommended adaptation of a systems methodology to development and evaluation of NPP training programs

  15. Patirtų negatyvių situacijų, depresiškumo ir įsitikinimų (pagal kognityvinę - elgesio teoriją) sąsajos su nuteistų vyrų tyčiniu savęs žalojimu

    OpenAIRE

    Jakutonytė, Jolanta

    2013-01-01

    Lietuvoje yra nedaug mokslinių tyrimų apie įkalintų asmenų tyčinį savęs žalojimą, susijusį su savižudybės rizika. Užsienio šalių moksliniais tyrimais nustatomos įkalintų asmenų tyčinio savęs žalojimo sąsajos su negatyviomis situacijomis ir depresiškumu yra nevienareikšmės. Įkalintų asmenų tyčinio savęs žalojimo atsiradimui gali būti svarbūs stabilesni asmenybiniai veiksniai, kaip kertiniai įsitikinimai. Tyrimo tikslas. Nustatyti patirtų negatyvių situacijų, depresiškumo ir kertini...

  16. Neural stem cell-derived exosomes mediate viral entry

    Directory of Open Access Journals (Sweden)

    Sims B

    2014-10-01

    Full Text Available Brian Sims,1,2,* Linlin Gu,3,* Alexandre Krendelchtchikov,3 Qiana L Matthews3,4 1Division of Neonatology, Department of Pediatrics, 2Department of Cell, Developmental, and Integrative Biology, 3Division of Infectious Diseases, Department of Medicine, 4Center for AIDS Research, University of Alabama at Birmingham, Birmingham, AL, USA *These authors contributed equally to this work Background: Viruses enter host cells through interactions of viral ligands with cellular receptors. Viruses can also enter cells in a receptor-independent fashion. Mechanisms regarding the receptor-independent viral entry into cells have not been fully elucidated. Exosomal trafficking between cells may offer a mechanism by which viruses can enter cells.Methods: To investigate the role of exosomes on cellular viral entry, we employed neural stem cell-derived exosomes and adenovirus type 5 (Ad5 for the proof-of-principle study. Results: Exosomes significantly enhanced Ad5 entry in Coxsackie virus and adenovirus receptor (CAR-deficient cells, in which Ad5 only had very limited entry. The exosomes were shown to contain T-cell immunoglobulin mucin protein 4 (TIM-4, which binds phosphatidylserine. Treatment with anti-TIM-4 antibody significantly blocked the exosome-mediated Ad5 entry.Conclusion: Neural stem cell-derived exosomes mediated significant cellular entry of Ad5 in a receptor-independent fashion. This mediation may be hampered by an antibody specifically targeting TIM-4 on exosomes. This set of results will benefit further elucidation of virus/exosome pathways, which would contribute to reducing natural viral infection by developing therapeutic agents or vaccines. Keywords: neural stem cell-derived exosomes, adenovirus type 5, TIM-4, viral entry, phospholipids

  17. Atmospheric Entry Studies for Venus Missions: 45 deg Sphere-Cone Rigid Aeroshells and Ballistic Entries

    Science.gov (United States)

    Prabu, Dinesh K.; Allen, Gary A., Jr.; Cappuccio, Gelsomina; Spilker, Thomas R.; Hwang, Helen H.; Moses, Robert W.

    2013-01-01

    The present study considers ballistic entries into the atmosphere of Venus using a 45deg sphere-cone rigid aeroshell, a legacy shape that has been used successfully in the past in the Pioneer Venus Multiprobe Mission. For a number of entry mass and capsule diameter combinations (i.e., various ballistic coefficients) and entry velocities, the trajectory space in terms of entry flight path angles between skip out and -30 is explored with a 3DOF trajectory code, TRAJ. Assuming that the thermal protection material of choice is carbon phenolic of flight heritage, the entry flight path angle space is constrained a posteriori by the mechanical and thermal performance parameters of the material. For mechanical performance, a 200 g limit is placed on the peak deceleration load and 10 bar is assumed as the limit for heritage carbon-phenolic material. It is shown that both constraints cannot be active simultaneously. For thermal performance, a heat flux 2.5 kW/sq cm is utilized as a threshold below which the heritage carbon phenolic is considered mass inefficient. Using these constraints, viable entry flight path angle corridors are determined. Analysis of the results also hints at the existence of a range of "critical" ballistic coefficients beyond which the steepest possible entries are determined by the pressure limit of 10 bar. The results are verified against known performance of the various probes used in the Pioneer Venus mission. It is anticipated that the results presented here will serve as a baseline in the development of a new class of ablative materials for future Venus missions.

  18. Attacking 22 entries in rugby union: running demands and differences between successful and unsuccessful entries.

    Science.gov (United States)

    Tierney, P; Tobin, D P; Blake, C; Delahunt, E

    2017-12-01

    Global Positioning System (GPS) technology is commonly utilized in team sports, including rugby union. It has been used to describe the average running demands of rugby union. This has afforded an enhanced understanding of the physical fitness requirements for players. However, research in team sports has suggested that training players relative to average demands may underprepare them for certain scenarios within the game. To date, no research has investigated the running demands of attacking 22 entries in rugby union. Additionally, no research has been undertaken to determine whether differences exist in the running intensity of successful and unsuccessful attacking 22 entries in rugby union. The first aim of this study was to describe the running intensity of attacking 22 entries. The second aim of this study was to investigate whether differences exist in the running intensity of successful and unsuccessful attacking 22 entries. Running intensity was measured using meters per minute (m min -1 ) for (a) total distance, (b) running distance, (c) high-speed running distance, and (d) very high-speed running distance. This study provides normative data for the running intensity of attacking 22 entries in rugby union. Forwards achieved greater high-speed running intensity in successful (3.6 m min -1 ) compared to unsuccessful (1.8 m min -1 ) attacking 22 entries. Forwards should try and achieve greater high-speed running intensity in attacking 22 entries to increase the likelihood of successful outcomes during this period of gameplay. © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  19. A role for heparan sulfate 3-O-sulfotransferase isoform 2 in herpes simplex virus type 1 entry and spread

    International Nuclear Information System (INIS)

    O'Donnell, Christopher D.; Tiwari, Vaibhav; Oh, Myung-Jin; Shukla, Deepak

    2006-01-01

    Heparan sulfate (HS) 3-O-sulfotransferase isoform-2 (3-OST-2), which belongs to a family of enzymes capable of generating herpes simplex virus type-1 (HSV-1) entry and spread receptors, is predominantly expressed in human brain. Despite its unique expression pattern, the ability of 3-OST-2 to mediate HSV-1 entry and cell-to-cell fusion is not known. Our results demonstrate that expression of 3-OST-2 can render Chinese hamster ovary K1 (CHO-K1) cells susceptible to entry of wild-type and mutant strains of HSV-1. Evidence for generation of gD receptors by 3-OST-2 were suggested by gD-mediated interference assay and the ability of 3-OST-2-expressing CHO-K1 cells to preferentially bind HSV-1 gD, which could be reversed by prior treatment of cells with HS lyases (heparinases II/III). In addition, 3-OST-2-expressing CHO-K1 cells acquired the ability to fuse with cells-expressing HSV-1 glycoproteins, a phenomenon that mimics a way of viral spread in vivo. Demonstrating specificity, the cell fusion was inhibited by soluble 3-O-sulfated forms of HS, but not unmodified HS. Taken together, our results raise the possibility of a role of 3-OST-2 in the spread of HSV-1 infection in the brain

  20. Liiketoiminnan kehittäminen 3D-tulostuksen avulla

    OpenAIRE

    Rissanen, Pauli; Pekkanen, Matti-Juhani

    2014-01-01

    Työn lähtökohtana oli liiketoiminnan kehittäminen 3d-tulostuksen avulla. Tätä ajatusta lähestyttiin kahdesta eri näkökulmasta, eli siitä miten tekijöiden oma yritys saisi rakennettua liiketoimintaa 3d-tulostuksen ympärille, ja siitä miten tekijät voisivat auttaa eri alojen toimijoita hyötymään 3d-tulostuksen mahdollisuuksista. Keskeisessä osassa opinnäytetyötä oli myös 3D-tulostuksen prosessin havainnollistaminen. Työn perustavava tavoitteena oli luoda tukeva perusta yrityksen toiminnalle...

  1. Uudistuva johtajuus ja Z-sukupolvi työelämässä

    OpenAIRE

    Aakkula, Eveliina

    2012-01-01

    Opinnäytetyössä käsitellään epäjohtamista, joka on uusi johtamisen käsite, jonka ovat luoneet Janne Tienari ja Rebekka Piekkari kirjassaan Z ja epäjohtaminen. Terminä epäjohtaminen ei ole virallinen, eikä tule välttämättä vakiintumaan johtajuuden käsitteiden joukkoon. Tämä uusi johtamisen malli liitetään vahvasti Z-sukupolven tulemiseen työmarkkinoille. Z-sukupolvea ovat 1990-luvulla syntyneet. Johtajuus on tärkeä aihe, jonka käsittelemiseen löytyy aina uusia näkökulmia, joiden toimivuutta vo...

  2. Entry into the nuclear pore complex is controlled by a cytoplasmic exclusion zone containing dynamic GLFG-repeat nucleoporin domains.

    Science.gov (United States)

    Fiserova, Jindriska; Spink, Matthew; Richards, Shane A; Saunter, Christopher; Goldberg, Martin W

    2014-01-01

    Nuclear pore complexes (NPCs) mediate nucleocytoplasmic movement. The central channel contains proteins with phenylalanine-glycine (FG) repeats, or variations (GLFG, glycine-leucine-phenylalanine-glycine). These are 'intrinsically disordered' and often represent weak interaction sites that become ordered upon interaction. We investigated this possibility during nuclear transport. Using electron microscopy of S. cerevisiae, we show that NPC cytoplasmic filaments form a dome-shaped structure enclosing GLFG domains. GLFG domains extend out of this structure and are part of an 'exclusion zone' that might act as a partial barrier to entry of transport-inert proteins. The anchor domain of a GLFG nucleoporin locates exclusively to the central channel. By contrast, the localisation of the GLFG domains varied between NPCs and could be cytoplasmic, central or nucleoplasmic and could stretch up to 80 nm. These results suggest a dynamic exchange between ordered and disordered states. In contrast to diffusion through the NPC, transport cargoes passed through the exclusion zone and accumulated near the central plane. We also show that movement of cargo through the NPC is accompanied by relocation of GLFG domains, suggesting that binding, restructuring and movement of these domains could be part of the translocation mechanism.

  3. The primeval fear of nuclear energy

    International Nuclear Information System (INIS)

    Broda, E.

    1979-01-01

    This text, written by E. Broda, in 1979 is about the primeval fear of nuclear energy. He analyses amongst other things apocalyptic visions of people, weapon plutonium from nuclear power plants, nuclear power plants in critical situations and the entry into the atomic age. (nowak)

  4. Using a Human Challenge Model of Infection to Measure Vaccine Efficacy: A Randomised, Controlled Trial Comparing the Typhoid Vaccines M01ZH09 with Placebo and Ty21a.

    Directory of Open Access Journals (Sweden)

    Thomas C Darton

    2016-08-01

    Full Text Available Typhoid persists as a major cause of global morbidity. While several licensed vaccines to prevent typhoid are available, they are of only moderate efficacy and unsuitable for use in children less than two years of age. Development of new efficacious vaccines is complicated by the human host-restriction of Salmonella enterica serovar Typhi (S. Typhi and lack of clear correlates of protection. In this study, we aimed to evaluate the protective efficacy of a single dose of the oral vaccine candidate, M01ZH09, in susceptible volunteers by direct typhoid challenge.We performed a randomised, double-blind, placebo-controlled trial in healthy adult participants at a single centre in Oxford (UK. Participants were allocated to receive one dose of double-blinded M01ZH09 or placebo or 3-doses of open-label Ty21a. Twenty-eight days after vaccination, participants were challenged with 104CFU S. Typhi Quailes strain. The efficacy of M01ZH09 compared with placebo (primary outcome was assessed as the percentage of participants reaching pre-defined endpoints constituting typhoid diagnosis (fever and/or bacteraemia during the 14 days after challenge. Ninety-nine participants were randomised to receive M01ZH09 (n = 33, placebo (n = 33 or 3-doses of Ty21a (n = 33. After challenge, typhoid was diagnosed in 18/31 (58.1% [95% CI 39.1 to 75.5] M01ZH09, 20/30 (66.7% [47.2 to 87.2] placebo, and 13/30 (43.3% [25.5 to 62.6] Ty21a vaccine recipients. Vaccine efficacy (VE for one dose of M01ZH09 was 13% [95% CI -29 to 41] and 35% [-5 to 60] for 3-doses of Ty21a. Retrospective multivariable analyses demonstrated that pre-existing anti-Vi antibody significantly reduced susceptibility to infection after challenge; a 1 log increase in anti-Vi IgG resulting in a 71% decrease in the hazard ratio of typhoid diagnosis ([95% CI 30 to 88%], p = 0.006 during the 14 day challenge period. Limitations to the study included the requirement to limit the challenge period prior to treatment to

  5. Tank Vapor Characterization Project: Headspace vapor characterization of Hanford Tank 241-TY-102: Results from samples collected on 04/12/96

    International Nuclear Information System (INIS)

    Evans, J.C.; Pool, K.H.; Thomas, B.L.

    1997-01-01

    This report describes the analytical results of vapor samples taken from the headspace of the waste storage tank 241-TY-102 (Tank TY-102) at the Hanford Site in Washington State. The results described in this report were obtained to'characterize the vapors present in the tank headspace and to support safety evaluations and tank farm operations. The results include air concentrations of selected inorganic and organic analytes, and grouped compounds from samples obtained by Westinghouse Hanford Company (WHC) and provided for analysis to Pacific Northwest National Laboratory (PNNL). Analyses were performed by the Vapor Analytical Laboratory (VAL) at PNNL. Analyte concentrations were based on analytical results and, where appropriate, sample volumes provided by WHC. A summary of the inorganic analytes, permanent gases, and total non-methane organic compounds is listed in Table S.1. The three highest concentration analytes detected in SUMMA trademark canister and triple sorbent trap samples are also listed in Table S.1. Detailed descriptions of the analytical results appear in the appendices

  6. Export of nuclear equipment and materials and the non-proliferation of nuclear weapons

    International Nuclear Information System (INIS)

    Courteix, Simone.

    1977-01-01

    The problem of the non-proliferation of nuclear weapons is one of great concern today despite the entry into force in the early '70s of the NPT. To master civilian nuclear technology implies the ability to develop nuclear explosive devices; therefore in recent years contacts have strengthened between countries exporting nuclear equipment, specially in the frame of the 'London Club' so as to ensure that their exports will not result in disseminating nuclear weapons. (NEA) [fr

  7. Abbreviations of nuclear power plant engineering

    International Nuclear Information System (INIS)

    Freyberger, G.H.

    1979-01-01

    The edition of this English and German list of abbreviations comprises about 5200 entries in English and about 1400 entries in German as well as the most important American, English, German and other foreign Utilities and component manufacturers frequently quoted in nuclear engineering literature and documentation. (orig./HP) [de

  8. Nuclear energy in the Philippines

    International Nuclear Information System (INIS)

    1977-03-01

    This bibliography was prepared by the Scientific Library, Nuclear Training Department of the Philippine Atomic Energy Commission for scientists and researchers interested in nuclear energy in the Philippines. This sixth supplement consists of eighty-six (86) entries, mostly research reports of the scientists of the Philippine Atomic Energy Commission. The entries are arranged alphabetically by titles under their respective subject headings together with their bibliographic data consisting of author, title of publication, volume, data and pages. A brief annotation or a summary of the article follows. An author index is provided to facilitate prompt retrieval of the particular research information

  9. Interaction between the G3 and L5 proteins of the vaccinia virus entry-fusion complex

    International Nuclear Information System (INIS)

    Wolfe, Cindy L.; Moss, Bernard

    2011-01-01

    The vaccinia virus entry-fusion complex (EFC) consists of 10 to 12 proteins that are embedded in the viral membrane and individually required for fusion with the cell and entry of the core into the cytoplasm. The architecture of the EFC is unknown except for information regarding two pair-wise interactions: A28 with H2 and A16 with G9. Here we used a technique to destabilize the EFC by repressing the expression of individual components and identified a third pair-wise interaction: G3 with L5. These two proteins remained associated under several different EFC destabilization conditions and in each case were immunopurified together as demonstrated by Western blotting. Further evidence for the specific interaction of G3 and L5 was obtained by mass spectrometry. This interaction also occurred when G3 and L5 were expressed in uninfected cells, indicating that no other viral proteins were required. Thus, the present study extends our knowledge of the protein interactions important for EFC assembly and stability.

  10. 3D-mallinnus ja hyödyntämismahdollisuudet

    OpenAIRE

    Knaapi, Ville; Juvani, Juho

    2012-01-01

    Opinnäytetyön tarkoituksena oli opetella luomaan 3D-malleja käyttäen 3ds Max Design 2010 -ohjelmistoa. Työn kohteena oli Lappeenrannassa sijaitseva Wolkoffin talomuseo, joka oli tarkoitus mallintaa virtuaalista esittelyä varten Etelä-Karjalan museolle. Työ suoritettiin projektityönä vuoden 2010 syksyn ja 2012 kesän välisenä aikana. Tekijöinä toimi kaksi Saimaan ammattikorkeakoulun tietotekniikan opiskelijaa. Aluksi museolta saatiin laaja toivomuslista virtuaalisen esitelmän sisältämistä t...

  11. Preliminary tank characterization report for single-shell tank 241-TY-102: best-basis inventory

    International Nuclear Information System (INIS)

    Place, D.E.

    1997-01-01

    This document is a preliminary Tank Characterization Report (TCR). It only contains the current best-basis inventory (Appendix D) for single-shell tank 241-TY-102. No TCRs have been previously issued for this tank, and current core sample analyses are not available. The best-basis inventory, therefore, is based on an engineering assessment of waste type, process flowsheet data, early sample data, and/or other available information. The Standard Inventories of Chemicals and Radionuclides in Hanford Site Tank Wastes describes standard methodology used to derive the tank-by-tank best-basis inventories. This preliminary TCR will be updated using this same methodology when additional data on tank contents become available

  12. US Ports of Entry

    Data.gov (United States)

    Department of Homeland Security — HSIP Non-Crossing Ports-of-Entry A Port of Entry is any designated place at which a CBP officer is authorized to accept entries of merchandise to collect duties, and...

  13. Preferential Ty1 retromobility in mother cells and nonquiescent stationary phase cells is associated with increased concentrations of total Gag or processed Gag and is inhibited by exposure to a high concentration of calcium.

    Science.gov (United States)

    Peifer, Andrew C; Maxwell, Patrick H

    2018-03-21

    Retrotransposons are abundant mobile DNA elements in eukaryotic genomes that are more active with age in diverse species. Details of the regulation and consequences of retrotransposon activity during aging remain to be determined. Ty1 retromobility in Saccharomyces cerevisiae is more frequent in mother cells compared to daughter cells, and we found that Ty1 was more mobile in nonquiescent compared to quiescent subpopulations of stationary phase cells. This retromobility asymmetry was absent in mutant strains lacking BRP1 that have reduced expression of the essential Pma1p plasma membrane proton pump, lacking the mRNA decay gene LSM1 , and in cells exposed to a high concentration of calcium. Mother cells had higher levels of Ty1 Gag protein than daughters. The proportion of protease-processed Gag decreased as cells transitioned to stationary phase, processed Gag was the dominant form in nonquiescent cells, but was virtually absent from quiescent cells. Treatment with calcium reduced total Gag levels and the proportion of processed Gag, particularly in mother cells. We also found that Ty1 reduced the fitness of proliferating but not stationary phase cells. These findings may be relevant to understanding regulation and consequences of retrotransposons during aging in other organisms, due to conserved impacts and regulation of retrotransposons.

  14. Verkkosovelluskehitys Laravel 5.3:lla

    OpenAIRE

    Nguyen, Anh-Vu

    2017-01-01

    Insinöörityössä tutustuttiin Laravel 5.3 Framework -sovelluskehykseen. Työn tavoitteena oli oppia Laravelin käyttöä, ja sitä käyttämällä nopeuttaa monimutkaisen ja turvallisen verkkosovelluksen luomista ja kehitystä. Laravel on avoimen lähdekoodin PHP-sovelluskehys, joka perustuu MVC-malliin. Sen tarkoituksena on helpottaa ja nopeuttaa verkkosovelluskehittäjän työtä. Samalla haluttiin luoda kaksi päiväkodeille suunnattua verkkosovellusta kyseistä sovelluskehystä käyttämällä. Halutuista ve...

  15. Texting while driving: is speech-based text entry less risky than handheld text entry?

    Science.gov (United States)

    He, J; Chaparro, A; Nguyen, B; Burge, R J; Crandall, J; Chaparro, B; Ni, R; Cao, S

    2014-11-01

    Research indicates that using a cell phone to talk or text while maneuvering a vehicle impairs driving performance. However, few published studies directly compare the distracting effects of texting using a hands-free (i.e., speech-based interface) versus handheld cell phone, which is an important issue for legislation, automotive interface design and driving safety training. This study compared the effect of speech-based versus handheld text entries on simulated driving performance by asking participants to perform a car following task while controlling the duration of a secondary text-entry task. Results showed that both speech-based and handheld text entries impaired driving performance relative to the drive-only condition by causing more variation in speed and lane position. Handheld text entry also increased the brake response time and increased variation in headway distance. Text entry using a speech-based cell phone was less detrimental to driving performance than handheld text entry. Nevertheless, the speech-based text entry task still significantly impaired driving compared to the drive-only condition. These results suggest that speech-based text entry disrupts driving, but reduces the level of performance interference compared to text entry with a handheld device. In addition, the difference in the distraction effect caused by speech-based and handheld text entry is not simply due to the difference in task duration. Copyright © 2014 Elsevier Ltd. All rights reserved.

  16. Aerothermodynamics of Blunt Body Entry Vehicles. Chapter 3

    Science.gov (United States)

    Hollis, Brian R.; Borrelli, Salvatore

    2011-01-01

    In this chapter, the aerothermodynamic phenomena of blunt body entry vehicles are discussed. Four topics will be considered that present challenges to current computational modeling techniques for blunt body environments: turbulent flow, non-equilibrium flow, rarefied flow, and radiation transport. Examples of comparisons between computational tools to ground and flight-test data will be presented in order to illustrate the challenges existing in the numerical modeling of each of these phenomena and to provide test cases for evaluation of Computational Fluid Dynamics (CFD) code predictions.

  17. 2009.3 Revision of the Evaluated Nuclear Data Library (ENDL2009.3)

    Energy Technology Data Exchange (ETDEWEB)

    Thompson, I. J. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Beck, B. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Descalle, M. A. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Mattoon, C. M. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Jurgenson, E. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

    2017-11-06

    LLNL's Computational Nuclear Data and Theory Group have created a 2009.3 revised release of the Evaluated Nuclear Data Library (ENDL2009.3). This library is designed to support LLNL's current and future nuclear data needs and will be employed in nuclear reactor, nuclear security and stockpile stewardship simulations with ASC codes. The ENDL2009 database was the most complete nuclear database for Monte Carlo and deterministic transport of neutrons and charged particles. It was assembled with strong support from the ASC PEM and Attribution programs, leveraged with support from Campaign 4 and the DOE/Office of Science's US Nuclear Data Program. This document lists the revisions and fixes made in a new release called ENDL2009.3, by com- paring with the existing data in the previous release ENDL2009.2. These changes are made in conjunction with the revisions for ENDL2011.3, so that both the .3 releases are as free as possible of known defects.

  18. Hotellin vastaanotto toimivana ja tehokkaana työympäristönä, case: Radisson Blu Espoo

    OpenAIRE

    Lahtinen, Mirjami

    2013-01-01

    Tämän opinnäytetyön toimeksiantajana toimi Otaniemessä sijaitseva Radisson Blu Espoo. Radisson Blu Espoo hotellissa on 209 huonetta ja suurin osa sen asiakkaista on business-matkailijoita, sen sijaitessa lähellä Keilarannan yrityskeskittymää. Radisson Blu kuuluu kansainväliseen Rezidor Hotel Group konserniin. Opinnäytetyön tarkoituksena oli selvittää erilaisia keinoja, joiden avulla hotellin vastaanotosta voidaan luoda toimivampi sekä kustannustehokkaampi. Toimivassa vastaanotossa työntekijät...

  19. Japanese evaluated nuclear data library version 3, JENDL-3

    International Nuclear Information System (INIS)

    Asami, Tetsuo; Igarashi, Shun-ichi; Ihara, Hitoshi

    1989-01-01

    The third version of Japanese Evaluated Nuclear Data Library, JENDL-3, has recently been compiled and issued. The major features of JENDL-3 are that it covers a much larger number of nuclides than the previous versions, that evaluation is made over the all energy regions from 10 -5 eV to 20 MeV using proper nuclear theories for each of the energy regions, and that it contains data on gamma ray generation. The present report first gives an outline of JENDL-3, and then describes the evaluation of nuclear data covering light nuclides, nuclides in structural materials, fission product nuclides, major actinide nuclides, transplutonium nuclides, and gamma ray generation. The applicability of JENDL-3 is examined through a variety of bench mark tests covering the fast reactor, thermal neutron reactor, shielding, neutronics of nuclear fusion reactor, dosimetry, and gamma ray generation data. The report also describes a library for calculation of decay heat, which has close relations with JENDL-3. It has been demonstrated that the experimental data given in this library are very reliable with high reproducibility. Additional activities planned for the future are also outlined briefly. (N.K.)

  20. Non-power application as an entry point to nuclear power program

    International Nuclear Information System (INIS)

    Nahrul Khair Alang Md Rashid

    2009-01-01

    Nuclear power is usually viewed as the flagship of nuclear technology. A nuclear power plant complex, visible and prominence, is iconic of the technology. That image makes its presence common knowledge to the extent that nuclear technology is equated almost totally with nuclear power by the general public. The downside of this visibility is that it becomes easy target in public misinformation programs. The non-power applications however are not visible, and devoid of icon. The non-power applications, therefore, can grow quite smoothly, attracting only little attention in the negative and in the positive senses. According to a study conducted in the USA in 2000 and in Japan in 2002, the socio-economic impact of non-power and power applications of nuclear technology are comparable. Involvement in non-power applications can be a good grounding for moving into power applications. This paper discusses the non-power nuclear technology applications and in what manner it can serve to prepare the introduction of nuclear power program. (Author)

  1. Border Crossing/Entry Data - Border Crossing/Entry Data Time Series tool

    Data.gov (United States)

    Department of Transportation — The dataset is known as “Border Crossing/Entry Data.” The Bureau of Transportation Statistics (BTS) Border Crossing/Entry Data provides summary statistics to the...

  2. Double entry bookkeeping vs single entry bookkeeping

    Directory of Open Access Journals (Sweden)

    Ileana Andreica

    2016-11-01

    Full Text Available Abstract: A financial management eficiently begin, primarily, with an accounting record kept in the best possible conditions, this being conditioned on the adoption of a uniform forms, rational, clear and simple accounting. Throughout history, there have been known two forms of accounting: the simple and double entry. Romanian society after 1990 underwent a substantial change in social structure, the sector on which put a great emphasis being private, that of small manufacturers, peddler, freelance, who work independently and authorized or as associative form (family enterprises, various associations (owners, tenants, etc., liberal professions, etc.. They are obliged to keep a simple bookkeeping, because they have no juridical personality. Companies with legal personality are required to keep double entry bookkeeping; therefore, knowledge and border demarcation between the two forms of organisation of accounting is an essential. The material used for this work is mainly represented by the financial and accounting documents, by the analysis of the economic, by legislative updated sources, and as the method was used the comparison method, using hypothetical data, in case of an authorized individual and a legal entity. Based on the chosen material, an authorized individual (who perform single entry accounting system and a juridical entity (who perform double entry accounting system were selected comparative case studies, using hypothetical data, were analysed advantages and disadvantages in term of fiscal, if using two accounting systems, then were highlighted some conclusion that result.

  3. Entry: direct control or regulation?

    NARCIS (Netherlands)

    Perotti, E.; Vorage, M.

    2009-01-01

    We model a setting in which citizens form coalitions to seek preferential entry to a given market. The lower entry the higher firm profits and political contributions, but the lower social welfare. Politicians choose to either control entry directly and be illegally bribed, or regulate entry using a

  4. Entry Descent and Landing Workshop Proceedings. Volume 1; Inflatable Reentry Vehicle Experiment-3 (IRVE-3) Project Overview & Instrumentation

    Science.gov (United States)

    Dillman, Robert

    2015-01-01

    Entry mass at Mars is limited by the payload size that can be carried by a rigid capsule that can fit inside the launch vehicle fairing. Landing altitude at Mars is limited by ballistic coefficient (mass per area) of entry body. Inflatable technologies allow payload to use full diameter of launch fairing, and deploy larger aeroshell before atmospheric interface, landing more payload at a higher altitude. Also useful for return of large payloads from Low Earth Orbit (LEO).

  5. Energy Return on Investment from Recycling Nuclear Fuel

    International Nuclear Information System (INIS)

    2011-01-01

    This report presents an evaluation of the Energy Return on Investment (EROI) from recycling an initial batch of 800 t/y of used nuclear fuel (UNF) through a Recycle Center under a number of different fuel cycle scenarios. The study assumed that apart from the original 800 t of UNF only depleted uranium was available as a feed. Therefore for each subsequent scenario only fuel that was derived from the previous fuel cycle scenario was considered. The scenarios represent a good cross section of the options available and the results contained in this paper and associated appendices will allow for other fuel cycle options to be considered.

  6. Pala-animaatiohahmojen animointi 2D- ja 3D-ympäristössä

    OpenAIRE

    Koivisto, Ida

    2011-01-01

    Opinnäytetyö käsittelee hahmoanimointia digitaalisessa pala-animaatiossa. Työssä keskitytään pääasiassa vertailemaan keskenään kahta hyvin erityyppistä ohjelmaa hahmoanimaattorin työskentely-ympäristöinä. Ohjelmat, joita opinnäytetyössä on käytetty animoimiseen, ovat 3D-ohjelma Cinema 4D sekä 2D-2.5D -ohjelma After Effects. Opinnäytetyö on kirjoitettu hahmoanimaattorin näkökulmasta perustuen käytännön animointityöskentelyyn lasten animaatiosarjassa Ella & Aleksi. Työn tekijä on toiminut p...

  7. Identification of pyrrolo[3,2-c]pyridin-4-amine compounds as a new class of entry inhibitors against influenza viruses in vitro

    International Nuclear Information System (INIS)

    Chang, So Young; Cruz, Deu John M.; Ko, Yoonae; Min, Ji-Young

    2016-01-01

    Various influenza virus entry inhibitors are being developed as therapeutic antiviral agents in ongoing preparation for emerging influenza viruses, particularly those that may possess drug resistance to the current FDA-approved neuraminidase inhibitors. In this study, small molecules having the pyrrolopyridinamine (PPA), aminothiadiazole (ATD), dihydrofuropyridine carboxamide (HPC), or imidazopyridinamine (IPA) moiety were selected from a target-focused chemical library for their inhibitory activity against influenza A virus by high-throughput screening using the PR8GFP assay. Activity was evaluated by measuring changes the proportion of GFP-expressing cells as a reflection of influenza virus infection. Among them, PPA showed broad-spectrum activity against multiple influenza A viruses and influenza B virus. PPA was found to block the early stages of influenza virus infection using a time-of-addition assay. Using additional phenotypic assays that dissect the virus entry process, it appears that the antiviral activity of PPA against influenza virus can be attributed to interference of the post-fusion process: namely, virus uncoating and nuclear import of viral nucleoprotein complexes. Based on these results, PPA is an attractive chemical moiety that can be used to develop new antiviral drug candidates against influenza viruses. - Highlights: • Four chemical classes are identified from target-focused chemical library by HTS. • PPA inhibits the infection of various influenza A viruses and influenza B virus. • PPA is identified to inhibit the early stages of influenza virus infection. • PPA compound disrupts virus uncoating and nuclear import of viral ribonucleoprotein.

  8. IDIS-menetelmäsalkku työntekijän ammattitaidon vahvistajana lastensuojelun identiteettityössä

    OpenAIRE

    Palm, David

    2014-01-01

    Opinnäytetyön tarkoituksena oli vahvistaa lastensuojelutyön työntekijän ammattitaitoa lasten ja nuorten kanssa tehtävässä identiteettityössä. Tavoitteena oli IDIS-menetelmäsalkun avulla toteuttaa ja testata pienryhmäkodissa toiminnallisia lasten ja nuorten arvon näkyväksi tekemiseen tähtääviä menetelmiä. IDIS-menetelmäsalkulla tavoiteltiin myös pysyvää toimintamenetelmää pienryhmäkodin arkeen. IDIS-menetelmäsalkku oli opinnäytetyön tekijän itse suunnittelema kokonaisuus. Tutkimukselli...

  9. Ei ruokaa, ei elokuvaa : elokuvacateringin analysointi ja yritystoiminnan perustaminen liiketoimintasuunnitelman ja henkilökohtaisen työkokemuksen avulla

    OpenAIRE

    Itkonen, Johanna

    2017-01-01

    Tässä opinnäytetyössä käsittelen elokuva-cateringiä (freelancer-toimintana) sen mahdollisuuksia ja toimenpiteitä kasvaa yritykseksi (Jojo Foods, työnimi) liiketoimintasuunnitelman avulla, sekä tarkastella mitä vaaditaan ja mitä henkilökohtaisesti toivon elokuvacatering -alan yritykseltäni tulevaisuudessa. Suomessa opinnäytetyön kirjoitushetkellä on vain yksi erityisesti elokuvatuotantoihin ja -kuvauksiin suunnattu catering-alan yritys, jonka omistaa ja pyörittää yksi ihminen. Freelance-toimij...

  10. Jesus' Entry into Jerusalem

    Directory of Open Access Journals (Sweden)

    Juho Sankamo

    2014-12-01

    Full Text Available This article intends to contribute to the understanding of Jesus’ entry into Jerusalem. The author studies the entry, which is found in all the Gospels, in its Jewish context. The author argues that Jesus’ entry into Jerusalem on an ass is to be understood as a prophetic sign which was primarily meant to convey a message to the Jews.

  11. Instructions for preparation of data entry sheets for Licensee Event Report (LER) file. Revision 1. Instruction manual

    International Nuclear Information System (INIS)

    1977-07-01

    The manual provides instructions for the preparation of data entry sheets for the licensee event report (LER) file. It is a revision to an interim manual published in October 1974 in 00E-SS-001. The LER file is a computer-based data bank of information using the data entry sheets as input. These data entry sheets contain pertinent information in regard to those occurrences required to be reported to the NRC. The computer-based data bank provides a centralized source of data that may be used for qualitative assessment of the nature and extent of off-normal events in the nuclear industry and as an index of source information to which users may refer for more detail

  12. Automated Re-Entry System using FNPEG

    Science.gov (United States)

    Johnson, Wyatt R.; Lu, Ping; Stachowiak, Susan J.

    2017-01-01

    This paper discusses the implementation and simulated performance of the FNPEG (Fully Numerical Predictor-corrector Entry Guidance) algorithm into GNC FSW (Guidance, Navigation, and Control Flight Software) for use in an autonomous re-entry vehicle. A few modifications to FNPEG are discussed that result in computational savings -- a change to the state propagator, and a modification to cross-range lateral logic. Finally, some Monte Carlo results are presented using a representative vehicle in both a high-fidelity 6-DOF (degree-of-freedom) sim as well as in a 3-DOF sim for independent validation.

  13. The entry of free radicals into polystyrene latex particles

    International Nuclear Information System (INIS)

    Adams, M.E.; Trau, M.; Gilbert, R.C.; Napper, D.R.

    1988-01-01

    Mechanistic understanding of the processes governing the kinetics of emulsion polymerization has both scientific and technical interest. One component of this process that is poorly understood at present is that of free radical entry into latex particles. Measurements were made of the entry rate coefficient as a function of temperature for free radicals entering polystyrene latex particles in seeded emulsion polymerizations initiated by γ-rays. The activation energy for entry was found to be less than 24 ± 3 kJ mol -1 , consistent with entry being controlled by a physical (e.g. diffusional) rather than a chemical process. Measurement of the entry rate coefficient as a function of the γ-ray dose rate suggested that the factors that determine the entry rate when the primary free radicals are uncharged are similar to those that determine the entry rate for charged free radicals derived from chemical initiation by peroxydisulfate. This result was consistent with measurements of the entry rate coefficient of charged free radicals derived from peroxydisulfate; these data were found to be virtually independent of both the extent of the latex surface coverage by the anionic surfactant sodium dodecyl sulfate and the ionic strength of the continuous phase. The data refute several proposals given in the literature for the rate-determining step for entry, being inconsistent with control by collision of free radicals with the latex particles, surfactant desorption, and an electrostatic barrier arising from the colloidal nature of the entering free radical. The origin of the activation energy for entry remains obscure

  14. Efficacy of Atovaquone plus Proguanil for Malaria Prophylaxis in Children, and its Effect on the Immunogenicity of Live Oral Cholera (CVD103-HgR) and Typhoid (Ty21a) Vaccines

    OpenAIRE

    Gruß, Holger

    2003-01-01

    Wir führten eine randomisierte Doppelblindstudie durch, um den Einfluss der Malariachemoprophy-laxe mit Atovaquon-Proguanil auf die Antikörperantwort auf die oralen Lebendimpfungen gegen Cholera (CVD103-HgR) und Typhus (Ty21a) zu bestimmen. Dazu wurden insgesamt 330 gabuni-sche Kinder den beiden Behandlungsgruppen A-P oder Placebo zugeteilt. In diesen verblieben sie während 12 Wochen Chemoprophylaxegabe und 4 Wochen Nachsorgephase. Die Impfungen mit den oben genannten Impfstoffen erfolgte 3 ...

  15. From Hiroshima to nuclear deterrence

    International Nuclear Information System (INIS)

    Villain, Jacques; Moret, Andre

    2015-01-01

    The authors first propose an overview of nuclear weapons from their origins to 2014: evolution of weapon systems between 1945 and 2014, main treaties related to nuclear weapons, main consequences of the nuclear revolution, brief presentation and assessment of defences against ballistic missiles, cost of nuclear warfare. The second part proposes a perspective for nuclear weapons in tomorrow's world as it seems that the World will remain nuclear: possible evolution of nuclear proliferation, evolution of risks related to nuclear weapons, desirable and possible evolution of nuclear weapons. Appendices propose presentations of the main techniques used for ballistic missiles: propulsion, control, navigation and guiding, atmosphere re-entry, multiple nuclear heads, data processing, relationship between nuclear plants and weapons

  16. Proceedings of NUCLEAR 2016 the 9th annual international conference on sustainable development through nuclear research and education. Part 3/3

    International Nuclear Information System (INIS)

    Paraschiv, Irina Maria

    2016-01-01

    The proceedings of the NUCLEAR 2016 the 9th annual international conference on sustainable development through nuclear research and education held at INR-Pitesti on May, 18-20, contain 81 communications presented in two plenary sessions and three sections addressing the themes of Nuclear energy, Environmental protection and Sustainable development. This section (Part 3/3) is addressing the following items: Section 3.1 Education, training and knowledge management (22 papers); Section 3.2 International cooperation (5 papers); These papers are presented as abstracts in 'Nuclear 2016 - Book of Abstracts', separately processed

  17. Verification of SAMG entry condition for APR1400

    Energy Technology Data Exchange (ETDEWEB)

    Yun, Junghyun; Kim, Taewan; Kim, Jonghyun [KEPCO International Nuclear Graduate School, Ulsan (Korea, Republic of)

    2013-10-15

    In the wake of the Fukushima accident, severe accident management has become important more than ever. While Emergency Operating Procedures (EOPs) focuses on cooling down the core during a design basis accident, the prime objective of severe accident management guideline (SAMG) is to prevent the release of radioactive material into the environment during a severe accident. Only one fixed value of core exit temperature (CET) has been applied for several decades to different types of nuclear power plants (NPPs) in Korea. Although the different types of NPPs have different cladding materials and system designs, the identical CET, i. e., 650 .deg. C, is applied as the entry condition to the SAMG of all the NPPs (except CANDU plants), i. e., Westinghouse type, OPR1000, and APR1400. In this paper, the transition point is re-evaluated for dominant severe accident sequences; station black-out (SBO), small break loss of coolant accident (SBLOCA), and me dium break loss of coolant accident (MBLOCA). In cases of SBO and SBLOCA, the current SAMG entry condition seems proper, while it needs to be reconsidered for MBLOCA case.

  18. 27 CFR 478.23 - Right of entry and examination.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 3 2010-04-01 2010-04-01 false Right of entry and... Administrative and Miscellaneous Provisions § 478.23 Right of entry and examination. (a) Except as provided in... of a criminal investigation of a person or persons other than the licensee, (2) For insuring...

  19. Japanese evaluated nuclear data library, version-3 - JENDL-3

    International Nuclear Information System (INIS)

    Kikuchi, Yasuyuki

    1992-01-01

    The third version of Japanese Evaluated Nuclear Data Library (JENDL-3) has been developed aiming at really general applications such as fission, fusion and shielding calculations. The general purpose file of JENDL-3 contains neutron nuclear data for 324 nuclides in the ENDF-5 format. In the JENDL-3 evaluation, much effort has been devoted to improve reliability of high-energy data for fusion application, which were not satisfactory in JENDL-2, and to include gamma-ray production data. Some advanced nuclear theoretical models were adopted and recent experimental data of energy-angle double-differential cross sections (DDX) mainly measured in Japan were taken into account. Various benchmark tests have so far been made in order to verify the applicability of JENDL-3 to various fields. For fast reactor calculations, JENDL-3 gives satisfactory results for most of characteristics. Particularly, space dependences of reaction rates, sodium void coefficients and control rod worths, which were significant with JENDL-2, nearly disappear. This suggests that the JENDL-3 data are well balanced. Satisfactory applicability has been also proved for the other field such as thermal reactor calculations, fusion neutronics, shielding and dosimetry. (orig.)

  20. Data entry system for INIS input using a personal computer

    International Nuclear Information System (INIS)

    Ishikawa, Masashi

    1990-01-01

    Input preparation for the INIS (International Nuclear Information System) has been performed by Japan Atomic Energy Research Institute since 1970. Instead of the input data preparation done by worksheets make out with the typewriters, new method with which data can be directly inputted into a diskette using personal computers is introduced. According to the popularization of personal computers and word processors, this system is easily applied to other system, so the outline and the future development on it are described. A shortcoming of this system is that spell-checking and data entry using authority files are hardly performed because of the limitation of hardware resources, and that data code conversion is needed because applied code systems between personal computer and main frame computer are quite different from each other. On the other hand, improving the timelyness of data entry is expected without duplication of keying. (author)

  1. 76 FR 40945 - Entergy Nuclear Indian Point 2, LLC, Entergy Nuclear Indian Point 3, LLC, Entergy Nuclear...

    Science.gov (United States)

    2011-07-12

    ... NUCLEAR REGULATORY COMMISSION [NRC-2011-0150; Docket Nos. 50-003, 50-247, and 50-286; License Nos. DPR-5, DPR-26, and DPR-64] Entergy Nuclear Indian Point 2, LLC, Entergy Nuclear Indian Point 3, LLC, Entergy Nuclear Operations, Inc.; Receipt of Request for Action Notice is hereby given that by petition...

  2. Border Crossing/Entry Data

    Data.gov (United States)

    Department of Transportation — The dataset is known as “Border Crossing/Entry Data.” The Bureau of Transportation Statistics (BTS) Border Crossing/Entry Data provides summary statistics to the...

  3. Air toxics evaluation for a nuclear power plant

    International Nuclear Information System (INIS)

    Lokey, D.; Orr, W.

    1994-01-01

    An emission inventory of nonradiological hazardous air pollutants (HAP) was prepared for TVA's Browns Ferry Nuclear Plant (BFN). The purpose of this effort was to determine whether BFN is a major HAP emission source. About 40 specific HAP were identified in products used at FBN. HAP emission totals from BFN were estimated at 6 T/Y, well below the major-source cutoff. Off-site waste disposal reduced gross emission estimates by about 8 percent. HAP emission evaluation by product categories showed the largest portion due to paints and coatings, followed by cleaning compounds. HAP emission breakdown by chemical species showed xylene to be the predominate HAP emitted, followed by methyl ethyl ketone

  4. Arachidonic acid-induced Ca2+ entry and migration in a neuroendocrine cancer cell line.

    Science.gov (United States)

    Goswamee, Priyodarshan; Pounardjian, Tamar; Giovannucci, David R

    2018-01-01

    Store-operated Ca 2+ entry (SOCE) has been implicated in the migration of some cancer cell lines. The canonical SOCE is defined as the Ca 2+ entry that occurs in response to near-maximal depletion of Ca 2+ within the endoplasmic reticulum. Alternatively, arachidonic acid (AA) has been shown to induce Ca 2+ entry in a store-independent manner through Orai1/Orai3 hetero-multimeric channels. However, the role of this AA-induced Ca 2+ entry pathway in cancer cell migration has not been adequately assessed. The present study investigated the involvement of AA-induced Ca 2+ entry in migration in BON cells, a model gastro-enteropancreatic neuroendocrine tumor (GEPNET) cell line using pharmacological and gene knockdown methods in combination with live cell fluorescence imaging and standard migration assays. We showed that both the store-dependent and AA-induced Ca 2+ entry modes could be selectively activated and that exogenous administration of AA resulted in Ca 2+ entry that was pharmacologically distinct from SOCE. Also, whereas homomeric Orai1-containing channels appeared to largely underlie SOCE, the AA-induced Ca 2+ entry channel required the expression of Orai3 as well as Orai1. Moreover, we showed that AA treatment enhanced the migration of BON cells and that this migration could be abrogated by selective inhibition of the AA-induced Ca 2+ entry. Taken together, these data revealed that an alternative Orai3-dependent Ca 2+ entry pathway is an important signal for GEPNET cell migration.

  5. Physics-Based Modeling of Meteor Entry and Breakup

    Science.gov (United States)

    Prabhu, Dinesh K.; Agrawal, Parul; Allen, Gary A., Jr.; Bauschlicher, Charles W., Jr.; Brandis, Aaron M.; Chen, Yih-Kang; Jaffe, Richard L.; Palmer, Grant E.; Saunders, David A.; Stern, Eric C.; hide

    2015-01-01

    A new research effort at NASA Ames Research Center has been initiated in Planetary Defense, which integrates the disciplines of planetary science, atmospheric entry physics, and physics-based risk assessment. This paper describes work within the new program and is focused on meteor entry and breakup.Over the last six decades significant effort was expended in the US and in Europe to understand meteor entry including ablation, fragmentation and airburst (if any) for various types of meteors ranging from stony to iron spectral types. These efforts have produced primarily empirical mathematical models based on observations. Weaknesses of these models, apart from their empiricism, are reliance on idealized shapes (spheres, cylinders, etc.) and simplified models for thermal response of meteoritic materials to aerodynamic and radiative heating. Furthermore, the fragmentation and energy release of meteors (airburst) is poorly understood.On the other hand, flight of human-made atmospheric entry capsules is well understood. The capsules and their requisite heatshields are designed and margined to survive entry. However, the highest speed Earth entry for capsules is 13 kms (Stardust). Furthermore, Earth entry capsules have never exceeded diameters of 5 m, nor have their peak aerothermal environments exceeded 0.3 atm and 1 kW/sq cm. The aims of the current work are: (i) to define the aerothermal environments for objects with entry velocities from 13 to 20 kms; (ii) to explore various hypotheses of fragmentation and airburst of stony meteors in the near term; (iii) to explore the possibility of performing relevant ground-based tests to verify candidate hypotheses; and (iv) to quantify the energy released in airbursts. The results of the new simulations will be used to anchor said risk assessment analyses. With these aims in mind, state-of-the-art entry capsule design tools are being extended for meteor entries. We describe: (i) applications of current simulation tools to

  6. Advertising and generic market entry.

    Science.gov (United States)

    Königbauer, Ingrid

    2007-03-01

    The effect of purely persuasive advertising on generic market entry and social welfare is analysed. An incumbent has the possibility to invest in advertising which affects the prescribing physician's perceived relative qualities of the brand-name and the generic version of the drug. Advertising creates product differentiation and can induce generic market entry which is deterred without differentiation due to strong Bertrand competition. However, over-investment in advertising can deter generic market entry under certain conditions and reduces welfare as compared to accommodated market entry.

  7. Nuclear energy policy issues after the 3.11 Fukushima nuclear accident

    Energy Technology Data Exchange (ETDEWEB)

    Suzuki, Tatsujiro [Japan Atomic Energy Commission (Japan)

    2014-07-01

    The Fukushima nuclear accident has become one of the worst accidents in nuclear history and it is not completely over yet. It will take at least 30 years or more to decontaminate and decommission the crippled nuclear reactors on site. Still, more than 140,000 people are away from home and restoring and assuring the life and welfare of those evacuated people is the top priority of Japanese government's nuclear energy policy. The government will release its new energy policy soon which will state that nuclear power is considered as an important base load electricity source, while committing to reduce its dependence as much as possible. For nuclear energy policy, there are certain important issues to be overcome regardless of future of nuclear power in Japan. They are: (1) spent fuel management and radioactive waste disposal, (2) restoring public trust (3) securing human resources and (4) plutonium stockpile management.

  8. der(4)t(Y;4): Three-generation transmission and sperm meiotic segregation analysis.

    Science.gov (United States)

    Vozdova, Miluse; Horinova, Vera; Wernerova, Vendula; Skalikova, Romana; Rybar, Roman; Prinosilova, Petra; Oracova, Eva; Rubes, Jiri

    2011-05-01

    We present a family where five members (three males and two females) are carriers of der(4)t(Y;4)(q11.23;p16.3). The adult carriers are phenotypicaly normal and fertile; the boy shows macrocephaly, psychomotor retardation, and atypical autism. The FISH on cultured lymphocytes confirmed that the redundant Yq heterochromatin was attached to the 4p-subtelomeric region maintained on the der(4). Sperm FISH analysis performed in a normospermic der(4) carrier showed a significant distortion of the expected 1:1 ratio of the X- and Y-bearing spermatozoa in favor of the X chromosome and significant lack of Y,der(4)spermatozoa. The overall lack of Y spermatozoa was not balanced even by a relative excess of Y,4 sperm. The analysis of X, Y, 7, 8, 18, and 21 sperm disomy and diploidy did not indicate any interchromosomal effect. The chromosome 4 disomy was significantly increased but still very low to be of considerable reproductive significance. The neurodevelomental phenotype of the boy was probably caused by a gene mutation. The coincidental occurrence of such chromosomal aberration and boy's phenotype might lead to misinterpretation of the causal relationship between these findings. It is necessary to consider the results of chromosomal analysis and clinical records of relatives for provide genetic counseling in such families. Copyright © 2011 Wiley-Liss, Inc.

  9. Flavivirus Entry Receptors: An Update

    Directory of Open Access Journals (Sweden)

    Manuel Perera-Lecoin

    2013-12-01

    Full Text Available Flaviviruses enter host cells by endocytosis initiated when the virus particles interact with cell surface receptors. The current model suggests that flaviviruses use at least two different sets of molecules for infectious entry: attachment factors that concentrate and/or recruit viruses on the cell surface and primary receptor(s that bind to virions and direct them to the endocytic pathway. Here, we present the currently available knowledge regarding the flavivirus receptors described so far with specific attention to C-type lectin receptors and the phosphatidylserine receptors, T-cell immunoglobulin and mucin domain (TIM and TYRO3, AXL and MER (TAM. Their role in flavivirus attachment and entry as well as their implication in the virus biology will be discussed in depth.

  10. Development of a stereolithography (STL input and computer numerical control (CNC output algorithm for an entry-level 3-D printer

    Directory of Open Access Journals (Sweden)

    Brown, Andrew

    2014-08-01

    Full Text Available This paper presents a prototype Stereolithography (STL file format slicing and tool-path generation algorithm, which serves as a data front-end for a Rapid Prototyping (RP entry- level three-dimensional (3-D printer. Used mainly in Additive Manufacturing (AM, 3-D printers are devices that apply plastic, ceramic, and metal, layer by layer, in all three dimensions on a flat surface (X, Y, and Z axis. 3-D printers, unfortunately, cannot print an object without a special algorithm that is required to create the Computer Numerical Control (CNC instructions for printing. An STL algorithm therefore forms a critical component for Layered Manufacturing (LM, also referred to as RP. The purpose of this study was to develop an algorithm that is capable of processing and slicing an STL file or multiple files, resulting in a tool-path, and finally compiling a CNC file for an entry-level 3- D printer. The prototype algorithm was implemented for an entry-level 3-D printer that utilises the Fused Deposition Modelling (FDM process or Solid Freeform Fabrication (SFF process; an AM technology. Following an experimental method, the full data flow path for the prototype algorithm was developed, starting with STL data files, and then processing the STL data file into a G-code file format by slicing the model and creating a tool-path. This layering method is used by most 3-D printers to turn a 2-D object into a 3-D object. The STL algorithm developed in this study presents innovative opportunities for LM, since it allows engineers and architects to transform their ideas easily into a solid model in a fast, simple, and cheap way. This is accomplished by allowing STL models to be sliced rapidly, effectively, and without error, and finally to be processed and prepared into a G-code print file.

  11. Proceedings of NUCLEAR 2013 the 6th annual international conference on sustainable development through nuclear research and education. Part 3/3

    International Nuclear Information System (INIS)

    Constantin, Marin; Turcu, Ilie

    2013-01-01

    The proceedings of the NUCLEAR 2013 international conference on sustainable development through nuclear research and education held at INR-Pitesti on May, 22-24 2013 contain 79 communications presented in two plenary sessions. The sections of conferecnce are adressing the following items: Section I.1 Nuclear Safety & Severe Accidents (15 papers), Section I.2 Nuclear Reactors & Generation IV (15 papers), Section I.3 Nuclear Technologies & Materials (28 papers), Section II.1 Radioprotection (6 papers), Section II.2 Radioactive Waste Management (8 papers), Section II.3 Air, Water & Soil Protection (5 papers) and Section III Sustainable Development (4 papers) These papers are presented as abstracts in 'Nuclear 2013 - BOOK of ABSTRACTS', separately processed

  12. Investigations of nuclear structure and nuclear reactions induced by complex projectiles

    International Nuclear Information System (INIS)

    Sarantites, D.G.

    1992-01-01

    The research program described touches five areas of nuclear physics: nuclear structure studies at high spin (hyperdeformation in the mass A ≅ 182 region, structure of 182 Hg and 182 Au at high spin, a highly deformed band in 136 Pm and the anomalous h 11/2 proton crossing in the A∼135 superdeformed region), studies at the interface between structure and reactions (population of entry states in heavy-ion fusion reactions, nuclear structure effects in proton evaporation spectra, nuclear structure- dependent entry state population by total spectroscopy, entrance channel effects in fusion near the barrier, lifetimes of subbarrier α particles by the atomic clock method), production and study of hot nuclei (the statistical model evaporation code EVAP, statistical emission of deuterons and tritons from highly excited compound nuclei, heavy-fragment emission as a probe of the thermal properties of highly excited compound nuclei, use of incoming-wave boundary condition transmission coefficients in the statistical model: implications in the particle evaporation spectra, study of transparency in the optical model), reaction mechanism studies (binary character of highly dissipative 209 Bi + 136 Xe collisions at E/A=28.2 MeV), and development and use of novel techniques and instrumentation in these areas of research (including a 4π channel selection device, a novel x-ray detector, and a simple channel-selecting detector)

  13. Japanese Evaluated Nuclear Data Library, version-3

    International Nuclear Information System (INIS)

    Shibata, Keiichi; Nakagawa, Tsuneo; Asami, Tetsuo

    1990-06-01

    The general purpose file of the third version of Japanese Evaluated Nuclear Data Library, JENDL-3, has been compiled by the JAERI Nuclear Data Center in cooperation with the Japanese Nuclear Data Committee. It contains neutron nuclear data for 171 nuclides which are needed for design of fission and fusion reactors and for shielding calculation. In the JENDL-3 evaluation, much effort was devoted to improve reliability of high-energy data for fusion application and to include gamma-ray production data. Theoretical calculations played an important role in achieving these purposes. A special method called simultaneous evaluation was adopted to determine important cross sections of fissile and fertile nuclides. This report presents a general description for the evaluation of light, medium-heavy and heavy nuclide data. Also given are the descriptive data for each nuclide contained in the File 1 part of JENDL-3. (author)

  14. Prm3p is a pheromone-induced peripheral nuclear envelope protein required for yeast nuclear fusion.

    Science.gov (United States)

    Shen, Shu; Tobery, Cynthia E; Rose, Mark D

    2009-05-01

    Nuclear membrane fusion is the last step in the mating pathway of the yeast Saccharomyces cerevisiae. We adapted a bioinformatics approach to identify putative pheromone-induced membrane proteins potentially required for nuclear membrane fusion. One protein, Prm3p, was found to be required for nuclear membrane fusion; disruption of PRM3 caused a strong bilateral defect, in which nuclear congression was completed but fusion did not occur. Prm3p was localized to the nuclear envelope in pheromone-responding cells, with significant colocalization with the spindle pole body in zygotes. A previous report, using a truncated protein, claimed that Prm3p is localized to the inner nuclear envelope. Based on biochemistry, immunoelectron microscopy and live cell microscopy, we find that functional Prm3p is a peripheral membrane protein exposed on the cytoplasmic face of the outer nuclear envelope. In support of this, mutations in a putative nuclear localization sequence had no effect on full-length protein function or localization. In contrast, point mutations and deletions in the highly conserved hydrophobic carboxy-terminal domain disrupted both protein function and localization. Genetic analysis, colocalization, and biochemical experiments indicate that Prm3p interacts directly with Kar5p, suggesting that nuclear membrane fusion is mediated by a protein complex.

  15. KPNB1 mediates PER/CRY nuclear translocation and circadian clock function.

    Science.gov (United States)

    Lee, Yool; Jang, A Reum; Francey, Lauren J; Sehgal, Amita; Hogenesch, John B

    2015-08-29

    Regulated nuclear translocation of the PER/CRY repressor complex is critical for negative feedback regulation of the circadian clock of mammals. However, the precise molecular mechanism is not fully understood. Here, we report that KPNB1, an importin β component of the ncRNA repressor of nuclear factor of activated T cells (NRON) ribonucleoprotein complex, mediates nuclear translocation and repressor function of the PER/CRY complex. RNAi depletion of KPNB1 traps the PER/CRY complex in the cytoplasm by blocking nuclear entry of PER proteins in human cells. KPNB1 interacts mainly with PER proteins and directs PER/CRY nuclear transport in a circadian fashion. Interestingly, KPNB1 regulates the PER/CRY nuclear entry and repressor function, independently of importin α, its classical partner. Moreover, inducible inhibition of the conserved Drosophila importin β in lateral neurons abolishes behavioral rhythms in flies. Collectively, these data show that KPNB1 is required for timely nuclear import of PER/CRY in the negative feedback regulation of the circadian clock.

  16. Problematika operací s cennými papíry a finančními deriváty

    OpenAIRE

    Tejnský, Radko

    2010-01-01

    1 Resume Název: Problematika operací s cennými papíry a finančními deriváty Title: The issues of operations with securities and financial derivatives The aim of this thesis is to discuss selected issues of operations with securities (such as stocks, bonds, promissory notes, etc.) and financial derivatives. A financial derivative (or derivative security) is a financial instrument whose value depends on the values of other, more basic underlying variables. Commonly issued and frequently traded ...

  17. International Nuclear Model. Volume 3. Program description

    International Nuclear Information System (INIS)

    Andress, D.

    1985-01-01

    This is Volume 3 of three volumes of documentation of the International Nuclear Model (INM). This volume presents the Program Description of the International Nuclear Model, which was developed for the Nuclear and Alternate Fuels Division (NAFD), Office of Coal, Nuclear, Electric and Alternate Fuels, Energy Information Administration (EIA), US Department of Energy (DOE). The International Nuclear Model (INM) is a comprehensive model of the commercial nuclear power industry. It simulates economic decisions for reactor deployment and fuel management decision based on an input set of technical economic and scenario parameters. The technical parameters include reactor operating characteristics, fuel cycle timing and mass loss factors, and enrichment tails assays. Economic parameters include fuel cycle costs, financial data, and tax alternatives. INM has a broad range of scenario options covering, for example, process constraints, interregional activities, reprocessing, and fuel management selection. INM reports reactor deployment schedules, electricity generation, and fuel cycle requirements and costs. It also has specialized reports for extended burnup and permanent disposal. Companion volumes to Volume 3 are: Volume 1 - Model Overview, and Volume 2 - Data Base Relationships

  18. Co-operative agreement for Arab States in Asia for Research, Development and Training Related to Nuclear Science and Technology (ARASIA). Entry into force

    International Nuclear Information System (INIS)

    2002-01-01

    The Co-operative Agreement for Arab States in Asia for Research, Development and Training related to Nuclear Science and Technology (ARASIA), pursuant to Article XII, entered into force upon receipt by the Director General of the Agency of notification of acceptance by three Arab Member States of the Agency in Asia, in accordance with Article XI, i.e. on 29 July 2002. The Agreement shall continue to be in force for a period of six years from the date of its entry into force and may be extended for further period(s) if the States Parties so agree. The text of the Agreement is reproduced in the Annex hereto for the information of all Member States. By 20 November 2002, there were 5 Parties to the above Agreement

  19. Optimal firm growth under the threat of entry

    Science.gov (United States)

    Kort, Peter M.; Wrzaczek, Stefan

    2015-01-01

    The paper studies the incumbent-entrant problem in a fully dynamic setting. We find that under an open-loop information structure the incumbent anticipates entry by overinvesting, whereas in the Markov perfect equilibrium the incumbent slightly underinvests in the period before the entry. The entry cost level where entry accommodation passes into entry deterrence is lower in the Markov perfect equilibrium. Further we find that the incumbent’s capital stock level needed to deter entry is hump shaped as a function of the entry time, whereas the corresponding entry cost, where the entrant is indifferent between entry and non-entry, is U-shaped. PMID:26435573

  20. Entry Threat and Entry Deterrence: The Timing of Broadband Rollout

    OpenAIRE

    Mo Xiao; Peter F. Orazem

    2007-01-01

    Past empirical literature provides strong evidence that competition increases when new firms enter a market. However, rarely have economists been able to examine how competition changes with the threat of entry. This paper uses the evolution of the zip code level market structure of facilities-based broadband providers from 1999 to 2004 to investigate how a firm adjusts its entry strategy when facing the threat of additional entrants. We identify the potential entrant into a local market as t...

  1. Old foes, new understandings: nuclear entry of small non-enveloped DNA viruses.

    Science.gov (United States)

    Fay, Nikta; Panté, Nelly

    2015-06-01

    The nuclear import of viral genomes is an important step of the infectious cycle for viruses that replicate in the nucleus of their host cells. Although most viruses use the cellular nuclear import machinery or some components of this machinery, others have developed sophisticated ways to reach the nucleus. Some of these have been known for some time; however, recent studies have changed our understanding of how some non-enveloped DNA viruses access the nucleus. For example, parvoviruses enter the nucleus through small disruptions of the nuclear membranes and nuclear lamina, and adenovirus tugs at the nuclear pore complex, using kinesin-1, to disassemble their capsids and deliver viral proteins and genomes into the nucleus. Here we review recent findings of the nuclear import strategies of three small non-enveloped DNA viruses, including adenovirus, parvovirus, and the polyomavirus simian virus 40. Copyright © 2015 Elsevier B.V. All rights reserved.

  2. Disruption of Microtubules Post-Virus Entry Enhances Adeno-Associated Virus Vector Transduction

    Science.gov (United States)

    Xiao, Ping-Jie; Mitchell, Angela M.; Huang, Lu; Li, Chengwen; Samulski, R. Jude

    2016-01-01

    Perinuclear retention of viral particles is a poorly understood phenomenon observed during many virus infections. In this study, we investigated whether perinuclear accumulation acts as a barrier to limit recombinant adeno-associated virus (rAAV) transduction. After nocodazole treatment to disrupt microtubules at microtubule-organization center (MT-MTOC) after virus entry, we observed higher rAAV transduction. To elucidate the role of MT-MTOC in rAAV infection and study its underlying mechanisms, we demonstrated that rAAV's perinuclear localization was retained by MT-MTOC with fluorescent analysis, and enhanced rAAV transduction from MT-MTOC disruption was dependent on the rAAV capsid's nuclear import signals. Interestingly, after knocking down RhoA or inhibiting its downstream effectors (ROCK and Actin), MT-MTOC disruption failed to increase rAAV transduction or nuclear entry. These data suggest that enhancement of rAAV transduction is the result of increased trafficking to the nucleus via the RhoA-ROCK-Actin pathway. Ten-fold higher rAAV transduction was also observed by disrupting MT-MTOC in brain, liver, and tumor in vivo. In summary, this study indicates that virus perinuclear accumulation at MT-MTOC is a barrier-limiting parameter for effective rAAV transduction and defines a novel defense mechanism by which host cells restrain viral invasion. PMID:26942476

  3. Entry and Exit.

    Science.gov (United States)

    1987-03-01

    1. Introduction R Analyses of industrial competition have attained a new vigor with the application of game -theoretic methods. The process of... competition is represented in models that reflect genuine struggles for entry, market power, and continuing survival. Dynamics and informational effects are...presents a few of the models developed recently to study competitive processes that affect a firm’s entry into a market , and the decision to exit. The

  4. 1984 Act on nuclear activities (1984:3)

    International Nuclear Information System (INIS)

    1984-01-01

    This 1984 Act on Nuclear Activities (1984:3) replaces the 1956 Atomic Energy Act as well as the 1977 Act on special permits to charge nuclear reactors with nuclear fuel and the 1980 Act on Public Insight into the Safety Work at Nuclear Power Plants. Like the 1956 Act, the 1984 Act in a safety legislation, which is based on a system of licensing conditions and supervision. According to the fundamental provisions of the 1984 Act, nuclear activities should be conducted in such a way as to meet safety requirements and fulfil the obligations that follow from Sweden's international agreements for the purpose of preventing the proliferation of nuclear weapons. (NEA) [fr

  5. 3 CFR 8342 - Proclamation 8342 of January 16, 2009. To Suspend Entry as Immigrants and Nonimmigrants of...

    Science.gov (United States)

    2010-01-01

    ... Entry as Immigrants and Nonimmigrants of Foreign Government Officials Responsible for Failing To Combat... January 16, 2009 Proc. 8342 To Suspend Entry as Immigrants and Nonimmigrants of Foreign Government... into the United States, as immigrants or nonimmigrants, of certain senior government officials...

  6. Strategic Entry Deterrence Modeling: Literature Review

    Directory of Open Access Journals (Sweden)

    D. A. Seliverstov

    2017-01-01

    Full Text Available The prime focus in this article is on key findings concerning theoretical aspects of strategic behavior by incumbents to deter market entry of new firms. The author summarizes main lines of scientific research in the topic which give an insight into the patterns of the incumbent’s impact on the behavior of the entrants, the entry deterrence instruments and the consequences of these actions. Today the free entry markets are considered to be a rare phenomenon. The market entry of new firms is associated with significant entry costs, which allow the incumbents to take advantage of their dominant position and derive positive economic profits. In case of entry threat by potential competitors the incumbents take strategic actions aimed at deterring entry and preserving their dominant position. Among the most efficient strategic actions one can emphasize the erection of additional barriers to entry for the newcomers through producing the limit output and price, investments in sunk assets, capacity expansion and product differentiation. Meanwhile by taking strategic actions the incumbents are not always trying to affect the entrant’s costs and profit directly, they often aim at changing the entrant’s expectations regarding future intentions of the incumbents to preserve dominant position.

  7. Nuclear structure references coding manual

    International Nuclear Information System (INIS)

    Ramavataram, S.; Dunford, C.L.

    1984-02-01

    This manual is intended as a guide to Nuclear Structure References (NSR) compilers. The basic conventions followed at the National Nuclear Data Center (NNDC), which are compatible with the maintenance and updating of and retrieval from the Nuclear Structure References (NSR) file, are outlined. The structure of the NSR file such as the valid record identifiers, record contents, text fields as well as the major topics for which [KEYWORDS] are prepared are ennumerated. Relevant comments regarding a new entry into the NSR file, assignment of [KEYNO ], generation of [SELECTRS] and linkage characteristics are also given. A brief definition of the Keyword abstract is given followed by specific examples; for each TOPIC, the criteria for inclusion of an article as an entry into the NSR file as well as coding procedures are described. Authors submitting articles to Journals which require Keyword abstracts should follow the illustrations. The scope of the literature covered at NNDC, the categorization into Primary and Secondary sources, etc. is discussed. Useful information regarding permitted character sets, recommended abbreviations, etc. is given

  8. Assessing welfare impact of entry into power market

    International Nuclear Information System (INIS)

    Cho, In-Koo; Kim, Hyunsook

    2013-01-01

    This paper calculates the welfare impact of a new entrant based on the location of entry in the Korean electricity market. We use two different models. One is the optimal fuel mix model to estimate the effect of a new entry in the long run. The other is the variable cost minimization model to assess the contribution of an existing installed private generator in the short run. A specific private generator, which has a cost advantage, saves a substantial amount of system-wide variable costs. We show that the right location for a new entrant can save power generation costs significantly, even if a new entrant does not have a cost advantage. - Highlights: • This paper calculates the welfare impact of a new entrant based on the location of entry. • We use two different models to estimate the entry effect. • The minimum and maximum cost savings of a new entrant are about 0.3% and 0.84% of total generation cost. • Even if a new entrant has no cost advantage, its choice of location could save money

  9. The cell surface expressed nucleolin is a glycoprotein that triggers calcium entry into mammalian cells

    International Nuclear Information System (INIS)

    Losfeld, Marie-Estelle; Khoury, Diala El; Mariot, Pascal; Carpentier, Mathieu; Krust, Bernard; Briand, Jean-Paul; Mazurier, Joel; Hovanessian, Ara G.; Legrand, Dominique

    2009-01-01

    Nucleolin is an ubiquitous nucleolar phosphoprotein involved in fundamental aspects of transcription regulation, cell proliferation and growth. It has also been described as a shuttling molecule between nucleus, cytosol and the cell surface. Several studies have demonstrated that surface nucleolin serves as a receptor for various extracellular ligands implicated in cell proliferation, differentiation, adhesion, mitogenesis and angiogenesis. Previously, we reported that nucleolin in the extranuclear cell compartment is a glycoprotein containing N- and O-glycans. In the present study, we show that glycosylation is an essential requirement for surface nucleolin expression, since it is prevented when cells are cultured in the presence of tunicamycin, an inhibitor of N-glycosylation. Accordingly, surface but not nuclear nucleolin is radioactively labeled upon metabolic labeling of cells with [ 3 H]glucosamine. Besides its well-demonstrated role in the internalization of specific ligands, here we show that ligand binding to surface nucleolin could also induce Ca 2+ entry into cells. Indeed, by flow cytometry, microscopy and patch-clamp experiments, we show that the HB-19 pseudopeptide, which binds specifically surface nucleolin, triggers rapid and intense membrane Ca 2+ fluxes in various types of cells. The use of several drugs then indicated that Store-Operated Ca 2+ Entry (SOCE)-like channels are involved in the generation of these fluxes. Taken together, our findings suggest that binding of an extracellular ligand to surface nucleolin could be involved in the activation of signaling pathways by promoting Ca 2+ entry into cells

  10. Stakeholder identification of advanced technology opportunities at international ports of entry

    Energy Technology Data Exchange (ETDEWEB)

    Parker, S.K. [Sandia National Labs., Albuquerque, NM (United States). Energy Policy and Planning Dept.; Icerman, L. [Icerman and Associates, Santa Fe, NM (United States)

    1997-01-01

    As part of the Advanced Technologies for International and Intermodal Ports of Entry (ATIPE) Project, a diverse group of stakeholders was engaged to help identify problems experienced at inland international border crossings, particularly those at the US-Mexican border. The fundamental issue at international ports of entry is reducing transit time through the required documentation and inspection processes. Examples of other issues or problems, typically manifested as time delays at border crossings, repeatedly mentioned by stakeholders include: (1) lack of document standardization; (2) failure to standardize inspection processes; (3) inadequate information and communications systems; (4) manual fee and tariff collection; (5) inconsistency of processes and procedures; and (6) suboptimal cooperation among governmental agencies. Most of these issues can be addressed to some extent by the development of advanced technologies with the objective of allowing ports of entry to become more efficient while being more effective. Three categories of technologies were unambiguously of high priority to port of entry stakeholders: (1) automated documentation; (2) systems integration; and (3) vehicle and cargo tracking. Together, these technologies represent many of the technical components necessary for pre-clearance of freight approaching international ports of entry. Integration of vehicle and cargo tracking systems with port of entry information and communications systems, as well as existing industry legacy systems, should further enable border crossings to be accomplished consistently with optimal processing times.

  11. Recruitment of phosphorylated small heat shock protein Hsp27 to nuclear speckles without stress

    International Nuclear Information System (INIS)

    Bryantsev, A.L.; Chechenova, M.B.; Shelden, E.A.

    2007-01-01

    During stress, the mammalian small heat shock protein Hsp27 enters cell nuclei. The present study examines the requirements for entry of Hsp27 into nuclei of normal rat kidney (NRK) renal epithelial cells, and for its interactions with specific nuclear structures. We find that phosphorylation of Hsp27 is necessary for the efficient entry into nuclei during heat shock but not sufficient for efficient nuclear entry under control conditions. We further report that Hsp27 is recruited to an RNAse sensitive fraction of SC35 positive nuclear speckles, but not other intranuclear structures, in response to heat shock. Intriguingly, Hsp27 phosphorylation, in the absence of stress, is sufficient for recruitment to speckles found in post-anaphase stage mitotic cells. Additionally, pseudophosphorylated Hsp27 fused to a nuclear localization peptide (NLS) is recruited to nuclear speckles in unstressed interphase cells, but wildtype and nonphosphorylatable Hsp27 NLS fusion proteins are not. The expression of NLS-Hsp27 mutants does not enhance colony forming abilities of cells subjected to severe heat shock, but does regulate nuclear speckle morphology. These data demonstrate that phosphorylation, but not stress, mediates Hsp27 recruitment to an RNAse soluble fraction of nuclear speckles and support a site-specific role for Hsp27 within the nucleus

  12. Beloyarsk Nuclear Power Plant

    International Nuclear Information System (INIS)

    1997-01-01

    The Beloyarsk Nuclear Power Plant (BNPP) is located in Zarechny, approximately 60 km east of Ekaterinberg along the Trans-Siberian Highway. Zarechny, a small city of approximately 30,000 residents, was built to support BNPP operations. It is a closed city to unescorted visitors. Residents must show identification for entry. BNPP is one of the first and oldest commercial nuclear power plants in Russia and began operations in 1964. As for most nuclear power plants in the Russian Federation, BNPP is operated by Rosenergoatom, which is subordinated to the Ministry of Atomic Energy of the Russian Federation (Minatom). BNPP is the site of three nuclear reactors, Units 1, 2, and 3. Units 1 and 2, which have been shut-down and defueled, were graphite moderated reactors. The units were shut-down in 1981 and 1989. Unit 3, a BN-600 reactor, is a 600 MW(electric) sodium-cooled fast breeder reactor. Unit 3 went on-line in April 1980 and produces electric power which is fed into a distribution grid and thermal power which provides heat to Zarechny. The paper also discusses the SF NIKIET, the Sverdiovsk Branch of NIKIET, Moscow, which is the research and development branch of the parent NIKEIT and is primarily a design institute responsible for reactor design. Central to its operations is a 15 megawatt IVV research reactor. The paper discusses general security and fissile material control and accountability at these two facilities

  13. AAV8 capsid variable regions at the two-fold symmetry axis contribute to high liver transduction by mediating nuclear entry and capsid uncoating

    International Nuclear Information System (INIS)

    Tenney, Rebeca M.; Bell, Christie L.; Wilson, James M.

    2014-01-01

    Adeno-associated virus serotype 8 (AAV8) is a promising vector for liver-directed gene therapy. Although efficient uncoating of viral capsids has been implicated in AAV8's robust liver transduction, much about the biology of AAV8 hepatotropism remains unclear. Our study investigated the structural basis of AAV8 liver transduction efficiency by constructing chimeric vector capsids containing sequences derived from AAV8 and AAV2 – a highly homologous yet poorly hepatotropic serotype. Engineered vectors containing capsid variable regions (VR) VII and IX from AAV8 in an AAV2 backbone mediated near AAV8-like transduction in mouse liver, with higher numbers of chimeric genomes detected in whole liver cells and isolated nuclei. Interestingly, chimeric capsids within liver nuclei also uncoated similarly to AAV8 by 6 weeks after administration, in contrast with AAV2, of which a significantly smaller proportion were uncoated. This study links specific AAV capsid regions to the transduction ability of a clinically relevant AAV serotype. - Highlights: • We construct chimeric vectors to identify determinants of AAV8 liver transduction. • An AAV2-based vector with 17 AAV8 residues exhibited high liver transduction in mice. • This vector also surpassed AAV2 in cell entry, nuclear entry and onset of expression. • Most chimeric vector particles were uncoated at 6 weeks, like AAV8 and unlike AAV2. • Chimera retained heparin binding and was antigenically distinct from AAV2 and AAV8

  14. AAV8 capsid variable regions at the two-fold symmetry axis contribute to high liver transduction by mediating nuclear entry and capsid uncoating

    Energy Technology Data Exchange (ETDEWEB)

    Tenney, Rebeca M.; Bell, Christie L.; Wilson, James M., E-mail: wilsonjm@mail.med.upenn.edu

    2014-04-15

    Adeno-associated virus serotype 8 (AAV8) is a promising vector for liver-directed gene therapy. Although efficient uncoating of viral capsids has been implicated in AAV8's robust liver transduction, much about the biology of AAV8 hepatotropism remains unclear. Our study investigated the structural basis of AAV8 liver transduction efficiency by constructing chimeric vector capsids containing sequences derived from AAV8 and AAV2 – a highly homologous yet poorly hepatotropic serotype. Engineered vectors containing capsid variable regions (VR) VII and IX from AAV8 in an AAV2 backbone mediated near AAV8-like transduction in mouse liver, with higher numbers of chimeric genomes detected in whole liver cells and isolated nuclei. Interestingly, chimeric capsids within liver nuclei also uncoated similarly to AAV8 by 6 weeks after administration, in contrast with AAV2, of which a significantly smaller proportion were uncoated. This study links specific AAV capsid regions to the transduction ability of a clinically relevant AAV serotype. - Highlights: • We construct chimeric vectors to identify determinants of AAV8 liver transduction. • An AAV2-based vector with 17 AAV8 residues exhibited high liver transduction in mice. • This vector also surpassed AAV2 in cell entry, nuclear entry and onset of expression. • Most chimeric vector particles were uncoated at 6 weeks, like AAV8 and unlike AAV2. • Chimera retained heparin binding and was antigenically distinct from AAV2 and AAV8.

  15. Structures and Mechanisms Design Concepts for Adaptive Deployable Entry Placement Technology

    Science.gov (United States)

    Yount, Bryan C.; Arnold, James O.; Gage, Peter J.; Mockelman, Jeffrey; Venkatapathy, Ethiraj

    2012-01-01

    System studies have shown that large deployable aerodynamic decelerators such as the Adaptive Deployable Entry and Placement Technology (ADEPT) concept can revolutionize future robotic and human exploration missions involving atmospheric entry, descent and landing by significantly reducing the maximum heating rate, total heat load, and deceleration loads experienced by the spacecraft during entry [1-3]. ADEPT and the Hypersonic Inflatable Aerodynamic Decelerator (HIAD) [4] share the approach of stowing the entry system in the shroud of the launch vehicle and deploying it to a much larger diameter prior to entry. The ADEPT concept provides a low ballistic coefficient for planetary entry by employing an umbrella-like deployable structure consisting of ribs, struts and a fabric cover that form an aerodynamic decelerator capable of undergoing hypersonic flight. The ADEPT "skin" is a 3-D woven carbon cloth that serves as a thermal protection system (TPS) and as a structural surface that transfers aerodynamic forces to the underlying ribs [5]. This paper focuses on design activities associated with integrating ADEPT components (cloth, ribs, struts and mechanisms) into a system that can function across all configurations and environments of a typical mission concept: stowed during launch, in-space deployment, entry, descent, parachute deployment and separation from the landing payload. The baseline structures and mechanisms were selected via trade studies conducted during the summer and fall of 2012. They are now being incorporated into the design of a ground test article (GTA) that will be fabricated in 2013. It will be used to evaluate retention of the stowed configuration in a launch environment, mechanism operation for release, deployment and locking, and static strength of the deployed decelerator. Of particular interest are the carbon cloth interfaces, underlying hot structure, (Advanced Carbon- Carbon ribs) and other structural components (nose cap, struts, and

  16. 1987 Annual convention of the Austrian Physical Society

    International Nuclear Information System (INIS)

    1987-09-01

    This is the pre-convention program of the annual convention to be held in September 1987. The divisions 1) general, 2) nuclear - and particle physics, 3) high-polymer physics have 124 contributions with abstracts, 75 of them of INIS interest. Another 22 contributions from the divisions, 4) atomic -, nuclear - and plasma physics, 5) solid state physics and 6) vocational training, are announced ty titles only. (G.Q.)

  17. Science, society, and America's nuclear waste: Unit 3, The Nuclear Waste Policy Act

    International Nuclear Information System (INIS)

    1992-01-01

    This is the 3rd unit, (The Nuclear Waste Policy Act) a four-unit secondary curriculum. It is intended to provide information about scientific and societal issues related to the management of spent nuclear fuel from generation of electricity at nuclear powerplants and high-level radioactive waste from US national defense activities. The curriculum, supporting classroom activities, and teaching materials present a brief discussion of energy and electricity generation, including that produced at nuclear powerplants; information on sources, amounts, location, and characteristics of spent nuclear fuel and high-level radioactive waste; sources, types and effects of radiation; US policy for managing and disposing of spent nuclear fuel and high-level radioactive waste and what other countries are doing; and the components of the nuclear waste management system

  18. Market Entry Strategies : Case: McDonald's entry on the Russian market

    OpenAIRE

    Karataev, Oleg

    2015-01-01

    The thesis considers the entry strategy and development of the company McDonald's into international markets. The theoretical aspects of the entry strategy of the company into the international markets. Analyzes the key features of the development of McDonald's in Russia. Investigated the prospects of the company in international markets. In theoretic part there was regarded some important aspects of international strategic management, such as: strategic alternatives, elements and levels o...

  19. Strategic Entry Deterrence Modeling: Literature Review

    OpenAIRE

    D. A. Seliverstov

    2017-01-01

    The prime focus in this article is on key findings concerning theoretical aspects of strategic behavior by incumbents to deter market entry of new firms. The author summarizes main lines of scientific research in the topic which give an insight into the patterns of the incumbent’s impact on the behavior of the entrants, the entry deterrence instruments and the consequences of these actions. Today the free entry markets are considered to be a rare phenomenon. The market entry of new firms is a...

  20. Characterizing Functional Domains for TIM-Mediated Enveloped Virus Entry

    Science.gov (United States)

    Moller-Tank, Sven; Albritton, Lorraine M.; Rennert, Paul D.

    2014-01-01

    ABSTRACT T-cell immunoglobulin and mucin domain 1 (TIM-1) and other TIM family members were recently identified as phosphatidylserine (PtdSer)-mediated virus entry-enhancing receptors (PVEERs). These proteins enhance entry of Ebola virus (EBOV) and other viruses by binding PtdSer on the viral envelope, concentrating virus on the cell surface, and promoting subsequent internalization. The PtdSer-binding activity of the immunoglobulin-like variable (IgV) domain is essential for both virus binding and internalization by TIM-1. However, TIM-3, whose IgV domain also binds PtdSer, does not effectively enhance virus entry, indicating that other domains of TIM proteins are functionally important. Here, we investigate the domains supporting enhancement of enveloped virus entry, thereby defining the features necessary for a functional PVEER. Using a variety of chimeras and deletion mutants, we found that in addition to a functional PtdSer-binding domain PVEERs require a stalk domain of sufficient length, containing sequences that promote an extended structure. Neither the cytoplasmic nor the transmembrane domain of TIM-1 is essential for enhancing virus entry, provided the protein is still plasma membrane bound. Based on these defined characteristics, we generated a mimic lacking TIM sequences and composed of annexin V, the mucin-like domain of α-dystroglycan, and a glycophosphatidylinositol anchor that functioned as a PVEER to enhance transduction of virions displaying Ebola, Chikungunya, Ross River, or Sindbis virus glycoproteins. This identification of the key features necessary for PtdSer-mediated enhancement of virus entry provides a basis for more effective recognition of unknown PVEERs. IMPORTANCE T-cell immunoglobulin and mucin domain 1 (TIM-1) and other TIM family members are recently identified phosphatidylserine (PtdSer)-mediated virus entry-enhancing receptors (PVEERs). These proteins enhance virus entry by binding the phospholipid, PtdSer, present on the viral

  1. 2011 Chemical, Biological, Radiological, and Nuclear Survivability Conference

    Science.gov (United States)

    2011-05-18

    Army – Europe (USAREUR) and Seventh Army as a Unit Commander responsible for Nuclear Surety on a NATO Nike Hercules AD Missile Site. In 1985, BG...International Corporation, Inc . (SAIC), McLean , Virginia Supported in the Pentagon (2004-2006) the Office of the DASD(NM), providing Staff and technical...Entry AIE II  Automated Installation Entry Increment 2 AVA  Anthrax Vaccine Adsorbed BAIS  Battlefield Anti-Intrusion System Bioscavenger Inc

  2. Science, society, and America's nuclear waste: Unit 3, The Nuclear Waste Policy Act

    International Nuclear Information System (INIS)

    1992-01-01

    This teachers guide is unit 3, the nuclear waste policy act, in a four-unit secondary curriculum. It is intended to provide information about scientific and societal issues related to the management of spent nuclear fuel from generation of electricity at nuclear powerplants and high-level radioactive waste from US national defense activities. The curriculum, supporting classroom activities, and teaching materials present a brief discussion of energy and electricity generation, including that produced at nuclear power plants; information on sources, amounts, location, and characteristics of spent nuclear fuel and high-level radioactive waste; sources, types and effects of radiation; US policy for managing and disposing of spent nuclear fuel and high-level radioactive waste and what other countries are doing; and the components of the nuclear waste management system

  3. Available hardware for automated entry control

    International Nuclear Information System (INIS)

    Holmes, J.P.

    1990-01-01

    Automated entry control has become an increasingly important issue at facilities where budget constraints are limiting options for manned entry control points. Ongoing work at Sandia National Laboratories is attempting to establish a data base for use by facility security managers working the problem of how to maintain security on a limited budget. Sandia National Laboratories conducted a performance test of the following biometric verifiers: (1) voice verifier by Alpha Microsystems of Santa Ana, California; (2) signature dynamics verifier by Autosig Systems of Irving, Texas; (3) voice verifier by Ecco Industries of Danvers, Massachusetts (now International Electronics); (4) retinal pattern verifier by EyeDentify of Portland, Oregon; (5) fingerprint verifier by Identix of Sunnyvale, California; and (6) hand geometry verifier by Recognition Systems of San Jose, California

  4. Strengthening the Convention on the Physical Protection of Nuclear Materials and Nuclear Facilities Regime: A Path Forward

    International Nuclear Information System (INIS)

    Pitts-Kiefer, S.; Nalabandian, M.

    2017-01-01

    With entry into force of the 2005 Amendment to the Convention on the Physical Protection of Nuclear Material (CPPNM) on May 8, 2016, and the culmination of the Nuclear Security Summits (NSS), the Convention on the Physical Protection of Nuclear Materials and Nuclear Facilities (CPP), as the amended convention is now known, can play an increasingly important role in efforts to strengthen the international nuclear security architecture. The CPP significantly enhances the international legal framework for nuclear security by expanding the scope of physical protection requirements and providing a direct linkage to International Atomic Energy Agency (IAEA) nuclear security guidance through incorporation of the Fundamental Principles.The CPP’s entry into force requires states parties to submit reports to the IAEA under Article 14 informing the IAEA of its laws and regulations giving effect to the convention—states parties were required to do so under the original CPPNM, but the scope of the reports will need to expand to reflect the expanded scope of the convention. Reporting builds confidence in the effectiveness of states’ security. In addition, entry into force of the CPP requires the IAEA, under Article 16, to convene a review conference in five years to assess the implementation and adequacy of the convention “in light of the then prevailing situation.” The review conference will provide an opportunity for states parties to assess the status of nuclear security progress and will provide a forum for dialogue on how to strengthen the global architecture and address remaining gaps in the system. Article 16 also provides for additional review conferences at periods of at least five years if requested by a majority of states parties. Regular review conferences would be an important mechanism for sustaining attention on nuclear security and ensuring continued nuclear security progress. For the CPP to fulfill its potential to play an important role in

  5. Safety of nuclear ships

    International Nuclear Information System (INIS)

    1978-01-01

    analysed. Three separate sessions were devoted to legal, licensing and port entry considerations. The participants discussed the legal aspects of sailing of nuclear merchant ships. Lawyers, members of nuclear ship crews, and members of port authorities discussed their past experience with the 'SAVANNAH' and 'OTTO HAHN', when each port entry had had to be negotiated at a high governmental level. The urgent need for internationally acceptable documents concerning the safety of nuclear ships and legal responsibilities in the case of accidents with nuclear ships were stressed

  6. Impact of Low-Energy Ion Beam Implantation on the Expression of Ty1-copia-like Retrotransposons in Wheat (Triticum aestivum)

    International Nuclear Information System (INIS)

    Ya Huiyuan; Jiao Zhen; Gu Yunhong; Wang Weidong; Qin Guangyong; Huo Yuping

    2007-01-01

    Retrotransposon-like elements are major constituents of most eukaryotic genomes. For example, they account for roughly 90% of the wheat (Triticum aestivum) genome. Previous study on a wheat strain treated by low-energy N + ions indicated the variations in AFLP (Amplified Fragment Length Polymorphism ) markers. One such variation was caused by the re-activation of Ty1-copia-like retrotransposons, implying that the mutagenic effects of low-energy ions might work through elevated activation of retrotransposons. In this paper an expression profile of Ty1-copia-like retrotransposons in wheat treated by low-energy N + ions is reported. The reverse transcriptase (RT) domains of these retrotransposons were amplified by reverse-transcriptional polymerase chain reaction (RT-PCR) and sequentially cloned. 42 and 65 clones were obtained from the treated (CL) and control materials (CK), respectively. Sequence analysis of each clone was performed by software. Phylogeny and classification were calculated responding to the sequences of the RT domains. All the results show that there is much difference in the RT domain between the control sample and the treated sample. Especially, the RT domains from the treated group encode significantly more functional ORF (open reading frames) than those from the control sample. This observation suggests that the treated sample has higher activation of retrotransposons, possibly as a consequence of low-energy ion beam irradiation. It also suggests that retrotransposons in the two groups impact the host gene expression in two different ways and carry out different functions in wheat cells

  7. Real-time multi-function entry / exit management system

    International Nuclear Information System (INIS)

    Hiyama, Kazuhisa; Kurosawa, Akihiko; Asano, Norikazu; Onoue, Ryuji; Eguchi, Shohei; Hanawa, Nobuhiro; Hori, Naohiko; Ueda, Hisao; Kanda, Hiroaki

    2012-01-01

    In order to prevent radiation accident and its expansion, more integrated management system is required to safety management for radiation workers in the nuclear facilities. Therefore, JAEA (Japan Atomic Energy Agency) and HAM (Hitachi Aloka Medical, Ltd) have developed innovative real-time multi-function entry/exit management system which managed worker's exposed dose and position under the joint developed patent. This system is sharing worker's data among workers and server manager who is inside of or outside of building, such as worker's positing, health condition and exposed dose. It consists of mobile equipments, receivers, LAN, and servers system. This report summarizes the system to be installed in the JMTR. (author)

  8. Trigeminal root entry zone involvement in neuromyelitis optica and multiple sclerosis.

    Science.gov (United States)

    Sugiyama, Atsuhiko; Mori, Masahiro; Masuda, Hiroki; Uchida, Tomohiko; Muto, Mayumi; Uzawa, Akiyuki; Ito, Shoichi; Kuwabara, Satoshi

    2015-08-15

    Trigeminal root entry zone abnormality on brain magnetic resonance imaging has been frequently reported in multiple sclerosis patients, but it has not been investigated in neuromyelitis optica patients. Brain magnetic resonance imaging of 128 consecutive multiple sclerosis patients and 46 neuromyelitis optica patients was evaluated. Trigeminal root entry zone abnormality was present in 11 (8.6%) of the multiple sclerosis patients and two (4.3%) of the neuromyelitis optica patients. The pontine trigeminal root entry zone may be involved in both multiple sclerosis and neuromyelitis optica. Copyright © 2015 Elsevier B.V. All rights reserved.

  9. Entry of soil gas and radon into houses

    International Nuclear Information System (INIS)

    Andersen, C.E.

    1992-04-01

    Entry of soil gas and radon into houses has been investigated by experiments conducted at radon test structures and numerical or analytical modelling. The numerical model solves the steady-state equations for Darcy flow of soil-gas and combined diffusive and advective transport of radon. Model calculations were compared with results from field experiments conducted at Risoe National Laboratory, and it was found that there was good agreement between measured and modelled pressure coupling and radon concentration profiles. Discrepancies regarding absolute values of soil-gas entry rates and radon concentrations were observed. The numerical model has been used to study the importance of soil and building related factors on radon entry rates into slab-on-grade houses. It was found that, for a house with a 3 mm perimeter crack along the floor-wall joint, the entry was mainly determined by the soil permeability and building related factors such as house depressurization and presence of a capillary breaking layer of gravel below the slab. In a house with a bare soil floor, the diffusivity of the soil was found to be of principal importance for the entry rate even for moderate permeabilities. An analytical model was developed for the purpose of studying soil-gas entry rates into houses in response to non-static driving forces. It is based on the analogy between a 'buried drain' and a basement house with a perimeter crack. The structure was depressurized sinusoidally in time and the frequency dependent pressure couplings were measured. There was fairly good agreement between theoretical and experimental results. (LN) (26 tabs., 30 ills., 66 refs.)

  10. 75 FR 24993 - Southern Nuclear Operating Company; Notice of Consideration of Issuance of Amendment to Early...

    Science.gov (United States)

    2010-05-06

    ... entry into ADAMS, which provides text and image files of NRC's public documents. If you do not have... searching on Docket ID: NRC-2008-0252. SUPPLEMENTARY INFORMATION: 1. Introduction The U.S. Nuclear... Electric Generating Plant Units 3 and 4 ESP site safety analysis report (SSAR) to allow the use and...

  11. Atmospheric Entry Studies for Uranus

    Science.gov (United States)

    Agrawal, P.; Allen, G. A.; Hwang, H. H.; Marley, M. S.; McGuire, M. K.; Garcia, J. A.; Sklyanskiy, E.; Huynh, L. C.; Moses, R. W.

    2014-07-01

    To better understand the technology requirements for Uranus atmospheric entry probe, Entry Vehicle Technology project funded an internal study with a multidisciplinary team from NASA Ames, Langley and JPL. The results of this study are communicated.

  12. Nuclear Regulatory Commission issuances. Volume 17, No. 3

    International Nuclear Information System (INIS)

    1983-03-01

    This report contains the Issuances received during March 1983 from the Commission (CLI), the Atomic Safety and Licensing Appeal Boards (ALAB), the Atomic Safety and Licensing Boards (LBP), the Administrative Law Judge (ALJ), the Directors Decisions (DD), and the Denials of Petition for Rulemaking (DPRM). The Issuances concerned the following facilities: Three Mile Island Nuclear Station, Unit No. 1; Comanche Peak Steam Electric Station, Units 1 and 2; Vallecitos Nuclear Center; Floating Nuclear Power Plants; San Onofre Nuclear Generating Station, Units 2 and 3; Point Beach Nuclear Plant, Unit 1; Perry Nuclear Power Plant, Units 1 and 2; Shoreham Nuclear Power Station, Unit 1; Western New York Nuclear Service Center; Limerick Generating Station, Units 1 and 2; Seabrook Station, Units 1 and 2; Black Fox Station, Units 1 and 2; WmH Zimmer Nuclear Power Station, Unit 1; WPPSS Nuclear Project No. 1; Zion Nuclear Plant, Units 1 and 2; and South Texas Project, Units 1 and 2

  13. Declaration by the governments of Argentina, Brazil and Chile on the entry into force for them of the treaty of Tlatelolco

    International Nuclear Information System (INIS)

    1992-01-01

    The document reproduces the text of a declaration by the Governments of Argentina, Brazil and Chile on the entry into force for them of the Treaty for the Prohibition of Nuclear Weapons in Latin America (Treaty of Tlatelolco), made on 26 August 1992 at the VII Special Session of the Agency for the Distribution of Nuclear Weapons in Latin America (OPANAL), held in Mexico City

  14. The depletion of nuclear glutathione impairs cell proliferation in 3t3 fibroblasts.

    Directory of Open Access Journals (Sweden)

    Jelena Markovic

    2009-07-01

    Full Text Available Glutathione is considered essential for survival in mammalian cells and yeast but not in prokaryotic cells. The presence of a nuclear pool of glutathione has been demonstrated but its role in cellular proliferation and differentiation is still a matter of debate.We have studied proliferation of 3T3 fibroblasts for a period of 5 days. Cells were treated with two well known depleting agents, diethyl maleate (DEM and buthionine sulfoximine (BSO, and the cellular and nuclear glutathione levels were assessed by analytical and confocal microscopic techniques, respectively. Both agents decreased total cellular glutathione although depletion by BSO was more sustained. However, the nuclear glutathione pool resisted depletion by BSO but not with DEM. Interestingly, cell proliferation was impaired by DEM, but not by BSO. Treating the cells simultaneously with DEM and with glutathione ethyl ester to restore intracellular GSH levels completely prevented the effects of DEM on cell proliferation.Our results demonstrate the importance of nuclear glutathione in the control of cell proliferation in 3T3 fibroblasts and suggest that a reduced nuclear environment is necessary for cells to progress in the cell cycle.

  15. Introductory user's manual for the US Nuclear Regulatory Commission Reactor Safety Research Data Bank. Revision 3

    International Nuclear Information System (INIS)

    Hardy, H.A.; Laats, E.T.

    1985-03-01

    The United States Nuclear Regulatory Commission (USNRC) has established the NRC/Division of Accident Evaluation (DAE) Data Bank Program to collect, store, and make available data from the many domestic and foreign water reactor safety research programs. Local direction of the program is provided by EG and G Idaho, Inc., prime contractor for the Department of Energy (DOE) at the Idaho National Engineering Laboratory (INEL). The NRC/DAE Data Bank Program provides a central computer storage mechanism and access software for data to be used by code development and assessment groups in meeting the code correlation needs of the nuclear industry. The administrative portion of the program provides data entry, documentation, training, and advisory services to users and the USNRC. The NRC/DAE Data Bank and the capabilities of the data access software are described in this document

  16. Hospitalisation of older people before and after long-term care entry in Auckland, New Zealand.

    Science.gov (United States)

    Boyd, Michal; Broad, Joanna B; Zhang, Tony Xian; Kerse, Ngaire; Gott, Merryn; Connolly, Martin J

    2016-07-01

    global population projections forecast large growth in demand for long-term care (LTC) and acute hospital services for older people. Few studies report changes in hospitalisation rates before and after entry into LTC. This study compares hospitalisation rates 1 year before and after LTC entry. the Older Persons' Ability Level (OPAL) study was a 2008 census-type survey of LTC facilities in Auckland, New Zealand. OPAL resident hospital admissions and deaths were obtained from routinely collected national databases. all 2,244 residents (66% = female) who entered LTC within 12 months prior to OPAL were included. There were 3,363 hospitalisations, 2,424 in 12 months before and 939 in 12 months after entry, and 364 deaths. In the 6 to 12 months before LTC entry, the hospitalisation rate/100 person-years was 67.3 (95% confidence interval [CI] 62.5-72.1). Weekly rates then rose steeply to over 450/100 person-years in the 6 months immediately before LTC entry. In the 6 months after LTC entry, the rate fell to 49.1 (CI 44.9-53.3; RR 0.73 (CI 0.65-0.82, P < 0.0001)) and decreased further 6 to 12 months after entry to 41.1 (CI 37.1-45.1; rate ratio [RR] 0.61 (CI 0.54-0.69, P < 0.0001)). increased hospitalisations a few months before LTC entry suggest functional and medical instability precipitates LTC entry. New residents utilise hospital beds less frequently than when at home before that unstable period. Further research is needed to determine effective interventions to avoid some hospitalisations and possibly also LTC entry. © The Author 2016. Published by Oxford University Press on behalf of the British Geriatrics Society. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  17. Predicting the Diversity of Foreign Entry Modes

    DEFF Research Database (Denmark)

    Hashai, Niron; Geisler Asmussen, Christian; Benito, Gabriel

    2007-01-01

    diversity across value chain activities and host markets. Analyzing a sample of Israeli based firms we show that larger firms exhibit a higher degree of entry mode diversity both across value chain activities and across host markets. Higher levels of knowledge intensity are also associated with more......This paper expands entry mode literature by referring to multiple modes exerted in different value chain activities within and across host markets, rather than to a single entry mode at the host market level. Scale of operations and knowledge intensity are argued to affect firms' entry mode...... diversity in firms' entry modes across both dimensions....

  18. Entry decisions in the generic pharmaceutical industry.

    Science.gov (United States)

    Morton, F M

    1999-01-01

    Data on all generic drug entries in the period 1984-1994 are used to estimate which markets heterogeneous potential entrants will decide to enter. I find that organizational experience predicts entry. Firms tend to enter markets with supply and demand characteristics similar to the firm's existing drugs. Larger revenue markets, markets with more hospital sales, and products that treat chronic conditions attract more entry. The simultaneous nature of entry leads to an additional interpretation: specialization is profitable because of the severe risk to profits when a market is "overentered." However, I am unable to make any conclusions about the efficiency of entry decisions.

  19. Nuclear merchant ship propulsion

    International Nuclear Information System (INIS)

    Schroeder, E.; Jager, W.; Schafstall, H.G.

    1977-01-01

    The operation of about 300 nuclear naval vessels has proven the feasibility of nuclear ship propulsion. Until now six non military ships have been built or are under construction. In the Soviet Union two nuclear icebreakers are in operation, and a third one is under construction. In the western world three prototype merchant ships have been built. Of these ships only the NS OTTO HAHN is in operation and provides valuable experience for future large scale use of nuclear merchant ship propulsion. In many countries studies and plans are made for future nuclear merchant ships. Types of vessels investigated are large containerships, tankers and specialized ships like icebreakers or ice-breaking ships. The future of nuclear merchant ship propulsion depends on three interrelated items: (1) nuclear ship technology; (2) economy of nuclear ship propulsion; (3) legal questions. Nuclear merchant ship technology is based until now on standard ship technology and light water reactor technology. Except for special questions due to the non-stationary type of the plant entirely new problems do not arise. This has been proven by the recent conceptual licensing procedure for a large nuclear containership in Germany. The economics of nuclear propulsion will be under discussion until they are proven by the operation of privately owned lead ships. Unsolved legal questions e.g. in connection with port entry permissions are at present another problem for nuclear shipping. Efforts are made to solve these questions on an international basis. The future development of nuclear energy electricity production in large land based plants will stimulate the employment of smaller units. Any future development of long distance sea transport will have to take this opportunity of a reliable and economic energy supply into account

  20. Nuclear receptor 4a3 (nr4a3 regulates murine mast cell responses and granule content.

    Directory of Open Access Journals (Sweden)

    Gianni Garcia-Faroldi

    Full Text Available Nuclear receptor 4a3 (Nr4a3 is a transcription factor implicated in various settings such as vascular biology and inflammation. We have recently shown that mast cells dramatically upregulate Nuclear receptor 4a3 upon activation, and here we investigated the functional impact of Nuclear receptor 4a3 on mast cell responses. We show that Nuclear receptor 4a3 is involved in the regulation of cytokine/chemokine secretion in mast cells following activation via the high affinity IgE receptor. Moreover, Nuclear receptor 4a3 negatively affects the transcript and protein levels of mast cell tryptase as well as the mast cell's responsiveness to allergen. Together, these findings identify Nuclear receptor 4a3 as a novel regulator of mast cell function.

  1. Survey of the population of the entry states in fusion reactons

    International Nuclear Information System (INIS)

    Sarantites, D.G.; Dilmanian, F.A.; Jaeaeskelaeinen, M.

    1982-01-01

    The population of the entry states from fusion reactions induced by 20 Ne and 50 Ti is investigated for a large number of reaction systems. Entrance channel l-distributions leading to fusion are deduced. The limiting angular momentum imparted to the evaporation residues is investigated as a function of Z and A. Fission plays a dominant role in determining the distributions in the evaporation residues. The effect of other mechanisms is also discussed. Prominent variations in the observed population distributions in (E*, M/sub γ/) space are attributed to nuclear structure effects

  2. Nucleoporin Nup98 mediates galectin-3 nuclear-cytoplasmic trafficking

    Energy Technology Data Exchange (ETDEWEB)

    Funasaka, Tatsuyoshi, E-mail: funasaka@staff.kanazawa-u.ac.jp [Laboratory of Molecular and Cellular Biology, Department of Biology, Faculty of Natural Systems, Institute of Science and Engineering, Kanazawa University, Ishikawa (Japan); Balan, Vitaly; Raz, Avraham [Department of Oncology and Pathology, Karmanos Cancer Institute, Wayne State University, School of Medicine, Detroit, MI (United States); Wong, Richard W., E-mail: rwong@staff.kanazawa-u.ac.jp [Laboratory of Molecular and Cellular Biology, Department of Biology, Faculty of Natural Systems, Institute of Science and Engineering, Kanazawa University, Ishikawa (Japan); Bio-AFM Frontier Research Center, Kanazawa Kanazawa University, Ishikawa (Japan)

    2013-04-26

    Highlights: •Nuclear pore protein Nup98 is a novel binding partner of galectin-3. •Nup98 transports galectin-3 into cytoplasm. •Nup98 depletion leads to galectin-3 nuclear transport and induces growth retardation. •Nup98 may involve in ß-catenin pathway through interaction with galectin-3. -- Abstract: Nucleoporin Nup98 is a component of the nuclear pore complex, and is important in transport across the nuclear pore. Many studies implicate nucleoporin in cancer progression, but no direct mechanistic studies of its effect in cancer have been reported. We show here that Nup98 specifically regulates nucleus–cytoplasm transport of galectin-3, which is a ß-galactoside-binding protein that affects adhesion, migration, and cancer progression, and controls cell growth through the ß-catenin signaling pathway in cancer cells. Nup98 interacted with galectin-3 on the nuclear membrane, and promoted galectin-3 cytoplasmic translocation whereas other nucleoporins did not show these functions. Inversely, silencing of Nup98 expression by siRNA technique localized galectin-3 to the nucleus and retarded cell growth, which was rescued by Nup98 transfection. In addition, Nup98 RNA interference significantly suppressed downstream mRNA expression in the ß-catenin pathway, such as cyclin D1 and FRA-1, while nuclear galectin-3 binds to ß-catenin to inhibit transcriptional activity. Reduced expression of ß-catenin target genes is consistent with the Nup98 reduction and the galectin-3–nucleus translocation rate. Overall, the results show Nup98’s involvement in nuclear–cytoplasm translocation of galectin-3 and ß-catenin signaling pathway in regulating cell proliferation, and the results depicted here suggest a novel therapeutic target/modality for cancers.

  3. Adaptive Text Entry for Mobile Devices

    DEFF Research Database (Denmark)

    Proschowsky, Morten Smidt

    The reduced size of many mobile devices makes it difficult to enter text with them. The text entry methods are often slow or complicated to use. This affects the performance and user experience of all applications and services on the device. This work introduces new easy-to-use text entry methods...... for mobile devices and a framework for adaptive context-aware language models. Based on analysis of current text entry methods, the requirements to the new text entry methods are established. Transparent User guided Prediction (TUP) is a text entry method for devices with one dimensional touch input. It can...... be touch sensitive wheels, sliders or similar input devices. The interaction design of TUP is done with a combination of high level task models and low level models of human motor behaviour. Three prototypes of TUP are designed and evaluated by more than 30 users. Observations from the evaluations are used...

  4. Trzyetapowa korekcja deformacji tyłostopia u chorej na reumatoidalne zapalenie stawów

    Directory of Open Access Journals (Sweden)

    Iwona Słowińska

    2011-12-01

    Full Text Available W artykule przedstawiono trzyetapowe leczenie operacyjne deformacjityłostopia u chorej na reumatoidalne zapalenie stawów (RZSw Klinice Reumoortopedii Instytutu Reumatologii w Warszawie.Z powodu znacznie zaawansowanych zmian destrukcyjnych wszystkichstawów na obu poziomach stawu skokowego konieczne byłowykonanie operacji przygotowującej do zaplanowanej endoprotezoplastykistawu skokowo-goleniowego. Wykonano potrójnąartrodezę tyłostopia, następnie reoperację usztywnienia z powodunadmiernej pierwotnej korekcji i ostatecznie aloplastykę stawuskokowego. Na rycinach 1–6 przedstawiono wyjściowy obraz zmiandestrukcyjnych stawów stopy pacjentki, kolejne etapy leczeniaoperacyjnego oraz zaistniałe powikłania. Pomimo powikłań dwóchpierwszych zabiegów chirurgicznych końcowy efekt był zadowa -lający. Obecnie pacjentka chodzi bez bólu w operowanej stopiei stawie skokowym, może nosić zwyczajne obuwie i jest zado wo -lona z wyglądu operowanej stopy.

  5. Attitude determination with three-axis accelerometer for emergency atmospheric entry

    Science.gov (United States)

    Garcia-Llama, Eduardo (Inventor)

    2012-01-01

    Two algorithms are disclosed that, with the use of a 3-axis accelerometer, will be able to determine the angles of attack, sideslip and roll of a capsule-type spacecraft prior to entry (at very high altitudes, where the atmospheric density is still very low) and during entry. The invention relates to emergency situations in which no reliable attitude and attitude rate are available. Provided that the spacecraft would not attempt a guided entry without reliable attitude information, the objective of the entry system in such case would be to attempt a safe ballistic entry. A ballistic entry requires three controlled phases to be executed in sequence: First, cancel initial rates in case the spacecraft is tumbling; second, maneuver the capsule to a heat-shield-forward attitude, preferably to the trim attitude, to counteract the heat rate and heat load build up; and third, impart a ballistic bank or roll rate to null the average lift vector in order to prevent prolonged lift down situations. Being able to know the attitude, hence the attitude rate, will allow the control system (nominal or backup, automatic or manual) to cancel any initial angular rates. Also, since a heat-shield forward attitude and the trim attitude can be specified in terms of the angles of attack and sideslip, being able to determine the current attitude in terms of these angles will allow the control system to maneuver the vehicle to the desired attitude. Finally, being able to determine the roll angle will allow for the control of the roll ballistic rate during entry.

  6. Abstracts of 3. congress of global anti-nuclear alliance

    International Nuclear Information System (INIS)

    2000-01-01

    The 3 congress of global anti-nuclear alliance was held on 18-20 May, 2000 in Astana. There were 55 reports on different aspects of nuclear disarmament; nuclear safeguards; safe operation of nuclear power plants; rehabilitation of population and lands, damaged from many-years tests in Semipalatinsk site; radiobiological monitoring of ecosystems and others presented at the congress

  7. Simulation of the ATV Re-Entry Obsrvations

    Science.gov (United States)

    Bastida Virgili, B.; Krag, H.; Lips, T.; De Pasquale, E.

    2010-09-01

    The first ATV was launched on 9th March 2008 and, after a successful mission, the last phase was a controlled destructive re-entry on 29th September 2008, shortly after 13:30 UTC, in which the remains of the ATV and its load fell into the South Pacific Ocean. In order to better understand the re-entry processes, an insitu optical observation campaign was launched to record and analyze the ATV controlled re-entry with several instruments on board of two airplanes and also from the ISS. This observation campaign was successful and triggered several different still-ongoing studies on the extraction and analysis of data to draw conclusions on the adequacy of the re-entry break-up and explosion models used for the safety analysis of the ATV re-entry. This paper addresses the validation process for ESA’s model for re-entry survivability and on-ground risk assessment for explosive re-entry events using the observation data. The underlying rationale is to improve the models for the benefit of planning and execution of future controlled re-entries and in risk calculation in case of uncontrolled ones. The re-entry trajectory of the ATV, the explosive event and the trajectories of the fragments are simulated with the existing ESA tools and the EVOLVE explosion model. Additional software has been developed to simulate airborne sensor field of view(FOV) crossings based on the aircraft trajectories, attitude profile, sensor mounts and FOVs. Sensor performance and object radiation are modeled in order to generate synthetic images for the different sensors in the ISS and the two airplanes. These synthetic images and synthetic videos are compared with the available reentry observations of the ATV. This paper will present the software and techniques to generate synthetic imagery. It will give results of the comparison between the simulated and the real trajectories and fragmentation and explain the subsequent validation process of the ESA re-entry tools and the potential

  8. Chemical engineering in fuel reprocessing. The French experience

    International Nuclear Information System (INIS)

    Viala, M.; Sombret, C.; Bernard, C.; Miquel, P.; Moulin, J.P.

    1992-01-01

    Reprocessing is the back-end of the nuclear fuel cycle, designed to recover valuable fissile materials, especially plutonium, and to condition safely all the wastes ready for disposal. For its new commercial reprocessing plants (UP 3 and UP 2 800) COGEMA decided to include many engineering innovations as well as new processes and key-components developed by CEA. UP 3 is a complete new plant with a capacity of 800 t/y which was put in operation in August 1990. UP 2 800 is an extension of the existing UP 2 facility, designed to achieve the same annual capacity of 800 t/y, to be put in operation at the end of 1993 by the commissioning of a new head-end and highly active chemical process facilities

  9. Protocol Additional to the agreement between the Republic of Armenia and the International Atomic Energy Agency for the application of safeguards in connection with the Treaty on the Non-Proliferation of Nuclear Weapons. Entry into force

    International Nuclear Information System (INIS)

    2004-01-01

    Pursuant to Article 17 of the Protocol Additional to the Agreement between the Republic of Armenia and the International Atomic Energy Agency for the Application of Safeguards in Connection with the Treaty on the Non-Proliferation of Nuclear Weapons, the Additional Protocol entered into force on 28 June 2004, the date on which the Agency received from Armenia written notification that Armenia's statutory and/or constitutional requirements for entry into force had been met

  10. Competitive Dynamics of Market Entry: Scale and Survival

    Directory of Open Access Journals (Sweden)

    John W. UPSON

    2017-06-01

    Full Text Available Market entry is the essence of strategy and is largely viewed as a dichotomous event: entry or no entry. What has not been acknowledged is the uniqueness of each market entry. Our study highlights the scale of market entry in the context of multipoint competition. We assert that entry scale varies based on the risk of market incumbent retaliation. Theory suggests that when risk associated with retaliation are low, firms enter with large scale and when associated risks are high, firms enter with low scale. Further, survival is viewed as dependent on following theory. We argue and find supporting evidence that firms behave in the opposite manner and do so to their own benefit, thereby revealing a unique discrepancy between theory and practice among 75 product market entries by 27 firms.

  11. The nuclear review

    International Nuclear Information System (INIS)

    Robinson, C.

    1995-01-01

    Evidence in favour of privatisation which was submitted to the UK government's review of the nuclear industry is summarised. Only if capital markets judge proposals for new nuclear stations and they operate within competitive markets, can their economic and commercial viability be determined. The problem of state ownership is that politicians' time horizons are too short for an industry that must take a long view, unless standards are set by competitors, costs are likely to rise. Magnox stations, however, should remain in public ownership though to gain efficiency their operation should be contracted out. The rest of Nuclear Electric and Scottish Nuclear should be privatised as generation companies but should not be tied to nuclear generation. Current protective arrangements should be removed as soon as possible. Among the benefits expected from the entry of two formidable new companies into the privatised electricity are efficiency gains, price reductions, a stimulus to entrepeneurship and innovation, and more straightforward regulation. (UK)

  12. CINDU-8 catalogue of numerical neutron data available from the IAEA Nuclear Data Unit

    Energy Technology Data Exchange (ETDEWEB)

    Qood, W M; Attree, P M; Byer, T A; Hjarne, L; Konshin, V A; Lemmel, H D; Lorenz, A [Nuclear Data Unit, International Atomic Energy Agency, Vienna (Austria)

    1969-01-15

    This catalogue is the index to DASTAR, the DAta STorage And Retrieval System of the IAEA Nuclear Data Unit. For the first time, this issue indexes also some parts of evaluated data libraries held at the Nuclear Data Unit. CINDU is written in a slightly modified CINDA format and should be read with the help of the introduction to CINDA. Tables of abbreviations for references and laboratories mentioned in this issue are given on the verso of the front cover. The catalogue serves as a reference to all the experimental and calculated numerical data which are stored at the IAEA Nuclear Data Unit, and each set of data is referred to by an index-number, e.g. DASTAR-00896. The bibliographic references for each experiment are blocked together with the relevant data index entry. All of the indexed data are available to anyone on request. Data should be ordered by their index number. This issue of CINDU is distributed to data centres, members of the International Nuclear Data Committee, correspondents to the IAEA Nuclear Data Unit, and others who have expressed their interest. Entries which have been added or changed since the issue of CINDU-7 are marked with an asterisk following the entry date.

  13. Foot-and-mouth disease virus induces autophagosomes during cell entry via a class III phosphatidylinositol 3-kinase-independent pathway.

    Science.gov (United States)

    Berryman, Stephen; Brooks, Elizabeth; Burman, Alison; Hawes, Philippa; Roberts, Rebecca; Netherton, Christopher; Monaghan, Paul; Whelband, Matthew; Cottam, Eleanor; Elazar, Zvulun; Jackson, Terry; Wileman, Thomas

    2012-12-01

    Autophagy is an intracellular pathway that can contribute to innate antiviral immunity by delivering viruses to lysosomes for degradation or can be beneficial for viruses by providing specialized membranes for virus replication. Here, we show that the picornavirus foot-and-mouth disease virus (FMDV) induces the formation of autophagosomes. Induction was dependent on Atg5, involved processing of LC3 to LC3II, and led to a redistribution of LC3 from the cytosol to punctate vesicles indicative of authentic autophagosomes. Furthermore, FMDV yields were reduced in cells lacking Atg5, suggesting that autophagy may facilitate FMDV infection. However, induction of autophagosomes by FMDV appeared to differ from starvation, as the generation of LC3 punctae was not inhibited by wortmannin, implying that FMDV-induced autophagosome formation does not require the class III phosphatidylinositol 3-kinase (PI3-kinase) activity of vps34. Unlike other picornaviruses, for which there is strong evidence that autophagosome formation is linked to expression of viral nonstructural proteins, FMDV induced autophagosomes very early during infection. Furthermore, autophagosomes could be triggered by either UV-inactivated virus or empty FMDV capsids, suggesting that autophagosome formation was activated during cell entry. Unlike other picornaviruses, FMDV-induced autophagosomes did not colocalize with the viral 3A or 3D protein. In contrast, ∼50% of the autophagosomes induced by FMDV colocalized with VP1. LC3 and VP1 also colocalized with the cellular adaptor protein p62, which normally targets ubiquitinated proteins to autophagosomes. These results suggest that FMDV induces autophagosomes during cell entry to facilitate infection, but not to provide membranes for replication.

  14. JENDL-3. The Japanese evaluated nuclear data library

    International Nuclear Information System (INIS)

    Lemmel, H.D.

    1992-01-01

    This document summarizes the contents of JENDL-3.1, the Japanese evaluated data library for neutron nuclear data, released in 1989 and revised in Dec. 1990. It also summarizes the JENDL-3 fission-products cross-section data library released in 1990. The entire library or retrievals of selected materials are available on magnetic tape from the IAEA Nuclear Data Section free of charge. (author)

  15. Structure-based function prediction of the expanding mollusk tyrosinase family

    Science.gov (United States)

    Huang, Ronglian; Li, Li; Zhang, Guofan

    2017-11-01

    Tyrosinase (Ty) is a common enzyme found in many different animal groups. In our previous study, genome sequencing revealed that the Ty family is expanded in the Pacific oyster ( Crassostrea gigas). Here, we examine the larger number of Ty family members in the Pacific oyster by high-level structure prediction to obtain more information about their function and evolution, especially the unknown role in biomineralization. We verified 12 Ty gene sequences from Crassostrea gigas genome and Pinctada fucata martensii transcriptome. By using phylogenetic analysis of these Tys with functionally known Tys from other molluscan species, eight subgroups were identified (CgTy_s1, CgTy_s2, MolTy_s1, MolTy-s2, MolTy-s3, PinTy-s1, PinTy-s2 and PviTy). Structural data and surface pockets of the dinuclear copper center in the eight subgroups of molluscan Ty were obtained using the latest versions of prediction online servers. Structural comparison with other Ty proteins from the protein databank revealed functionally important residues (HA1, HA2, HA3, HB1, HB2, HB3, Z1-Z9) and their location within these protein structures. The structural and chemical features of these pockets which may related to the substrate binding showed considerable variability among mollusks, which undoubtedly defines Ty substrate binding. Finally, we discuss the potential driving forces of Ty family evolution in mollusks. Based on these observations, we conclude that the Ty family has rapidly evolved as a consequence of substrate adaptation in mollusks.

  16. Journalismin uudet vuorovaikutuskeinot : Eli kuinka joukkoälyä käytetään osana toimituksellista työprosessia

    OpenAIRE

    Riihinen, Eleonoora

    2012-01-01

    Joukkoäly journalistisessa prosessissa on vielä vähän kokeiltu toimitustyökalu, joka yhdistää yleisön hyödyntämisen, tutkivan journalismin ja verkon datasovellukset. Joukkoälyllä tarkoitetaan jonkin tehtävän ulkoistamista ennalta nimeämättömälle joukolle ihmisiä. Tutkielmassani selvitän, millainen prosessi joukkoälyn käyttö on journalistisessa työprosessissa ja kuinka kiinnostuneita pienemmän levikkialueen maakuntalehdet ovat käyttämään yleisöä osana journalistista prosessia uusia toimitu...

  17. ”Minun ääneni” : Lapsilähtöisiä työskentelymenetelmiä Länsi-Pohjan keskussairaalan erityispoliklinikalle

    OpenAIRE

    Takalahti, Milla; Takalo-Kastari, Saara

    2012-01-01

    Opinnäytetyön kuvaus: Opinnäytetyömme koostuu menetelmäpaketista sekä opinnäytetyöraportista, joka sisältää teoriaosuuden ja opinnäytetyöprosessin kuvauksen. Tavoitteenamme oli edistää lapsilähtöisten menetelmien käyttöä erityispoliklinikalla ja edesauttaa vuorovaikutusta palveluohjaajan ja lapsen tai nuoren välillä. Valmistimme lapsilähtöisiä työskentelymenetelmiä sisältävän menetelmäpaketin Länsi-Pohjan keskussairaalan erityispoliklinikalle. Teoreettinen ja käsitteellinen esittely: ...

  18. 19 CFR 151.41 - Information on entry summary.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 2 2010-04-01 2010-04-01 false Information on entry summary. 151.41 Section 151... Products § 151.41 Information on entry summary. On the entry summary for petroleum or petroleum products in.... If the exact volumetric quantity cannot be determined in advance, the entry summary may be made for...

  19. Facilitating the entry into force and implementation of the Amendment to the Convention on the Physical Protection of Nuclear Material: Observations, challenges and benefits

    International Nuclear Information System (INIS)

    Johnson, Peri Lynne; )

    2014-01-01

    Amendment to enter into force is clear and its benefits are recognised, further consideration needs to be given to identifying why it has not yet entered into force. More particularly, what are the reasons why nearly half of the current CPPNM states parties, including those with and without nuclear material and nuclear facilities, have still not joined the Amendment? Also, what challenges do they face in joining and effectively implementing the instrument? This article will seek to provide answers and also to identify some benefits of the Amendment. Part A of this article places the Amendment into context by identifying the relevant legal instruments comprising the international legal framework for nuclear security. Part B highlights some of the Amendment's new and extended provisions. The IAEA Secretariat's internal Plan of Action on Facilitating Adherence to and Implementation of the Amendment (the 'Plan of Action') is highlighted in Part C, which also addresses relevant IAEA activities by identifying the broad range of services provided to IAEA member states. Part D identifies some observations and challenges associated with the entry into force and implementation of the Amendment, the main focus is on its provisions, which are likely to require changes to the national legislative framework. Finally, Part E of this article concludes with some identified potential benefits of joining the Amendment, in particular, those considered by the IAEA Advisory Group on Nuclear Security ('AdSec'). (author)

  20. Workforce re-entry for Japanese unemployed dental hygienists.

    Science.gov (United States)

    Usui, Y; Miura, H

    2015-02-01

    The aim of this study was to define the profile of unemployed dental hygienists who could be enticed to re-enter the workforce and the factors that could facilitate their re-entry into the dental field in Japan. The questionnaire was mailed with a postage-paid return envelope to a sample of 3095 licensed dental hygienists. A 50.4% response rate (S = 1477) was observed. The rate of working dental hygienists was 60.3% (n = 891), and of unemployed dental hygienists was 39.7% (n = 586). Of the latter, 31.9% (n = 187) stated intentions of returning to the workplace. The unemployed dental hygienists seeking employment were more often married and had more children, compared with working dental hygienists currently. This group also had significantly fewer total service years. Moreover, only 11.96% of them belonged to the Japan Dental Hygienists' Association, and 41.3% of those attended training workshops. According to their response, they perceived their top three major barriers to re-entry as 'lack sufficient dental hygiene skill', 'child rearing' and 'poor working atmosphere'. 'Flexibility in the work schedule' and 'location' were the most important factors for re-entry from their perspective. There were not many dental hygienists hoping to return to the dental field. The findings suggested that strategies to encourage non-practicing dental hygienists to re-entry should be emphasized in the areas of a flexible working atmosphere, easy access to information on how to return to practice and guidance on how to maintain professionalism during inactivity. © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  1. 3.International conference 'Nuclear and Radiation Physics'

    International Nuclear Information System (INIS)

    2001-01-01

    The 3-rd International Conference 'Nuclear and Radiation Physics' was held in Almaty (Kazakhstan) 4-7 June 2001. The primary purpose of the conference is consolidation of the scientists efforts in the area of fundamental and applied investigations on nuclear physics, radiation physics of solids and radioecology. In the conference more than 350 papers were presented by participants from 17 countries

  2. CINDU catalogue of numerical neutron data available from the IAEA Nuclear Data Unit

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1968-05-15

    This catalogue is the index to DASTAR, the DAta STorage And Retrieval System of the IAEA Nuclear Data Unit. It is written in a slightly modified CINDA format and should be read with the help of the introduction to CINDA, Tables of abbreviations for references and laboratories mentioned in this issue are given on the verso of the front cover. The catalogue serves as a reference to all the experimental and calculated numerical data which have been entered into DASTAR, and each set of data i s indexed with a DASTAR-number, e.g. DASTAR-00434. The bibliographic references for each experiment are blocked together with the relevant DASTAR-number entry. All of the indexed data are available to anyone on request. Data should be ordered by their DASTAR-number. This issue of CINDU is distributed to data centres, members of the International Nuclear Data Committee, correspondents to the IAEA Nuclear Data Unit, and others who have expressed their interest. Entries which have been added or changed since the issue of CINDU-6 are marked with an asterisk following the entry date.

  3. Nuclear manpower planning and personnel training

    International Nuclear Information System (INIS)

    Chen, J.H.

    1984-01-01

    Taiwan Power Company has established a nuclear manpower program to identify human resources, selection and recruitment of entry level engineers and technicians of Nuclear Energy Group. The methodology to estimate the future nuclear manpower demand of Taipower has been clearly described in this article. Also, the manpower program is being used as the bases for nuclear training program development. For safe, reliable and efficient operation of nuclear power plants, Taipower has established a systematic training program for nuclear power stations and headquarter personnel. The training program has been implemented in three stages with different patterns of training program. The first stage of nuclear training before 1975 was completed successfully. The second stage of nuclear training currently conducted since 1975 enlarges domestic training capability. The third stage of nuclear training with a long term training program is now under a systematic and compositive development effort

  4. Protoparvovirus Knocking at the Nuclear Door.

    Science.gov (United States)

    Mäntylä, Elina; Kann, Michael; Vihinen-Ranta, Maija

    2017-10-02

    Protoparvoviruses target the nucleus due to their dependence on the cellular reproduction machinery during the replication and expression of their single-stranded DNA genome. In recent years, our understanding of the multistep process of the capsid nuclear import has improved, and led to the discovery of unique viral nuclear entry strategies. Preceded by endosomal transport, endosomal escape and microtubule-mediated movement to the vicinity of the nuclear envelope, the protoparvoviruses interact with the nuclear pore complexes. The capsids are transported actively across the nuclear pore complexes using nuclear import receptors. The nuclear import is sometimes accompanied by structural changes in the nuclear envelope, and is completed by intranuclear disassembly of capsids and chromatinization of the viral genome. This review discusses the nuclear import strategies of protoparvoviruses and describes its dynamics comprising active and passive movement, and directed and diffusive motion of capsids in the molecularly crowded environment of the cell.

  5. Comparison of single-entry and double-entry two-step couple screening for cystic fibrosis carriers

    NARCIS (Netherlands)

    tenKate, LP; Verheij, JBGM; Wildhagen, MF; Hilderink, HBM; Kooij, L; Verzijl, JG; Habbema, JDF

    1996-01-01

    Both single-entry two-step (SETS) couple screening and double-entry two-step (DETS) couple screening have been recommended as methods to screen for cystic fibrosis gene carriers. In this paper we compare the expected results from both types of screening. In general, DETS results in a higher

  6. Entry Mode Choice in Emerging Markets

    OpenAIRE

    Gundersen, Anne Kathrine Navestad

    2012-01-01

    As the mature markets of developed economies have become increasingly saturated, firms are turning their attention towards emerging markets for further enterprise growth. However, these countries often present significant challenges for foreign entrants, forcing firms to adapt their strategies to the new context. While MNEs? entry mode choice is an extensively studied field, there is a deficit in the entry mode research on SMEs, and even more so when it comes to entry into emerging markets in...

  7. Analysis on Internal Control of TY Company ＆ Its System Reconstruction%TY公司内部控制现状分析和体系重构

    Institute of Scientific and Technical Information of China (English)

    黄美珍; 陈文芳

    2012-01-01

    在对福州TY公司进行深入调查的基础上,参照我国的《内部控制基本规范》及其配套指引,从控制环境、风险评估、控制活动、信息与沟通以及内部监督等方面对TY公司内部控制现状进行分析,对TY公司内部控制系统重构提出了自己的见解,以期能够对TY公司解决其内部控制问题有所启示。%Based on an in-depth study of TY Company,we analyze the current state of its internal control from the aspects of internal environment,risk assessment,internal monitoring,information and communication,referring to China＇s Basic Norms of Internal Control and its supporting guidelines.We put forward our views on internal control system reconstruction of TY Company,in hopes of offering some enlightening suggestions for resolving its internal control problems.

  8. Financial Performance of Entry Mode Decisions

    DEFF Research Database (Denmark)

    Boyd, Britta; Dyhr Ulrich, Anna Marie; Hollensen, Svend

    2012-01-01

    Based on a survey of 170 Danish SMEs the paper examines influences on entry mode choices and the financial outcome of these decisions. The main research objectives are divided into two steps: Step 1: To determine the factors influencing the choice of foreign entry modes by Danish companies. Step ...... and implications are provided for companies willing to invest more into foreign markets in order to achieve a higher degree of control and better financial results.......Based on a survey of 170 Danish SMEs the paper examines influences on entry mode choices and the financial outcome of these decisions. The main research objectives are divided into two steps: Step 1: To determine the factors influencing the choice of foreign entry modes by Danish companies. Step 2......: To determine the relationship between the choice of entry mode and export performance, measured in terms of financial outcome. Drawing from transaction cost theory the authors develop and test a model where different factors affect the level of control chosen by the parent company. This study contributes...

  9. IAEA advisory group meeting on basic and applied problems of nuclear level densities

    International Nuclear Information System (INIS)

    Bhat, M.R.

    1983-06-01

    Separate entries were made in the data base for 17 of the 19 papers included. Two papers were previously included in the data base. Workshop reports are included on (1) nuclear level density theories and nuclear model reaction cross-section calculations and (2) extraction of nuclear level density information from experimental data

  10. Study on entry criteria for severe accident management during hot leg LBLOCAs in a PWR

    International Nuclear Information System (INIS)

    Zhang, Longfei; Zhang, Dafa; Wang, Shaoming

    2007-01-01

    The risk of Large Break Loss of Coolant Accidents (LBLOCA) has been considered an important safety issue since the beginning of the nuclear power industry. The rapid depressurization occurs in the primary coolant circuit when a large break appears in a Pressurized Water Reactors (PWR).Then the coolant temperature reaches saturation at a very low pressure. The core outlet fluid temperatures maybe not reliable indicators of the core damage states at a such lower pressure. The problem is how to decide the time for water injection in the SAM (Severe Accident Management). An alternative entry criterion is the fluid temperature just above the hot channel in which the fluid temperature showed maximum among all the channels. For that reason, a systematic study of entry criterion of SAM for different hot leg break sizes in a 3-loop PWR has been started using the detailed system thermal hydraulic and severe accident analysis code package, RELAP/SCDAPSIM. Best estimate calculations of the large break LOCA of 15 cm, 20 cm and 25 cm without accident managements and in the case of high-pressure safety injection as the accident management were performed in this paper. The analysis results showed that the core exit temperatures are not reliable indicators of the peak core temperatures and core damage states once peak core temperatures reach 1500 K, and the proposed entry criteria for SAM at the time when the core outlet temperature reaches 900 K is not effective to prevent core melt. Then other analyses were performed with a parameter of fluid temperature just above the hot channel. The latter analysis showed that earlier water injection when the fluid temperature just above the hot channel reaches 900 K is effective to prevent further core melt. Since fuel surface and hot channel have spatial distribution and depend on a period of cycle operation, a series of thermocouples are required to install just above the fuel assembly. The maximum exit temperature of 900 K that captured by

  11. Models of radon entry: A review

    International Nuclear Information System (INIS)

    Gadgil, A.J.

    1991-08-01

    This paper reviews existing models of radon entry into houses. The primary mechanism of radon entry in houses with high indoor concentrations is, in most cases, convective entry of radon bearing soil-gas from the surrounding soil. The driving force for this convective entry is the small indoor-outdoor pressure difference arising from the stack effect and other causes. Entry points for the soil-gas generally are the cracks or gaps in the building substructure, or though other parts of the building shell in direct contact with the soil, although entry may also occur by flow though permeable concrete or cinder block walls of the substructure. Models using analytical solutions to idealized geometrical configurations with simplified boundary conditions obtain analytical tractability of equations to be solved at the cost of severe approximations; their strength is in the insights they offer with their solutions. Models based on lumped parameters attempt to characterize the significant physical behavioral characteristics of the soil-gas and radon flow. When realistic approximations are desired for the boundary conditions and terms in the governing equations, numerical models must be used; these are usually based on finite difference or finite element solutions to the governing equations. Limited data are now available for experimental verification of model predictions. The models are briefly reviewed and their strengths and limitations are discussed

  12. Major Cyber threat on Nuclear Facility and Key Entry Points of Malicious Codes

    Energy Technology Data Exchange (ETDEWEB)

    Shin, Ickhyun; Kwon, Kookheui [Korea Institute of Nuclear Nonproliferation and Control, Daejeon (Korea, Republic of)

    2013-05-15

    Cyber security incident explicitly shows that the domestic intra net system which is not connected to the Internet can be compromised by the USB based mal ware which was developed by the state-sponsored group. It also tells that the actor for cyber-attack has been changed from script kiddies to state's governments and the target has been changed to nation's main infrastructures such as electricity, transportation and etc. Since the cyber sabotage on nuclear facility has been proven to be possible and can be replicated again with same method, the cyber security on nuclear facility must be strengthened. In this paper, it is explained why the malicious code is the one of the biggest cyber threat in nuclear facility's digital I and C(Instrumentation and Controls) system by analyzing recent cyber attacks and well-known malicious codes. And a feasible cyber attack scenario on nuclear facility's digital I and C system is suggested along with some security measures for prevention of malicious code. As experienced from the cyber sabotage on Iranian nuclear facility in 2010, cyber attack on nuclear facility can be replicated by infecting the computer network with malicious codes. One of the cyber attack scenario on nuclear digital I and C computer network with using malicious code was suggested to help security manager establishing cyber security plan for prevention of malicious code. And some security measures on prevention of malicious code are also provided for reference.

  13. Major Cyber threat on Nuclear Facility and Key Entry Points of Malicious Codes

    International Nuclear Information System (INIS)

    Shin, Ickhyun; Kwon, Kookheui

    2013-01-01

    Cyber security incident explicitly shows that the domestic intra net system which is not connected to the Internet can be compromised by the USB based mal ware which was developed by the state-sponsored group. It also tells that the actor for cyber-attack has been changed from script kiddies to state's governments and the target has been changed to nation's main infrastructures such as electricity, transportation and etc. Since the cyber sabotage on nuclear facility has been proven to be possible and can be replicated again with same method, the cyber security on nuclear facility must be strengthened. In this paper, it is explained why the malicious code is the one of the biggest cyber threat in nuclear facility's digital I and C(Instrumentation and Controls) system by analyzing recent cyber attacks and well-known malicious codes. And a feasible cyber attack scenario on nuclear facility's digital I and C system is suggested along with some security measures for prevention of malicious code. As experienced from the cyber sabotage on Iranian nuclear facility in 2010, cyber attack on nuclear facility can be replicated by infecting the computer network with malicious codes. One of the cyber attack scenario on nuclear digital I and C computer network with using malicious code was suggested to help security manager establishing cyber security plan for prevention of malicious code. And some security measures on prevention of malicious code are also provided for reference

  14. Application of an entry-exit tariff model to the gas transport system in Spain

    International Nuclear Information System (INIS)

    Alonso, Alejandro; Serrano, Miguel; Olmos, Luis

    2010-01-01

    Under an entry-exit gas tariff system, reservation of capacity is split into entry capacity, to transport gas from the injection points to a virtual balancing point, and exit capacity, to transport gas from the balancing point to the exit points in the system. Entry-exit tariff for gas transport systems have been recommended by the 3rd EU Energy Package, since they are cost reflective, facilitate gas trade and can provide signals for the location of gas injections or off-takes. The advisability of applying an entry-exit tariff system is discussed in this paper. Apart from this, authors propose an entry-exit tariff model and apply it to compute charges for the Spanish gas transport system in 2009. Results produced by the model are presented as coefficients which should multiply the current postal transport tariff. The paper concludes that entry-exit tariffs would be useful location signals which would result in a better use of the gas transport system in Spain. In those cases where demand exceeds available capacity, as it occurs at the congested connection with France, entry-exit tariffs could be supplemented by capacity charges at entry points resulting from auctions. (author)

  15. 32 CFR 643.11 - Rights of entry.

    Science.gov (United States)

    2010-07-01

    ... 32 National Defense 4 2010-07-01 2010-07-01 true Rights of entry. 643.11 Section 643.11 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY (CONTINUED) REAL PROPERTY REAL ESTATE General § 643.11 Rights of entry. Pending the signing of the formal instrument, no right of entry will be...

  16. Nipah virus entry can occur by macropinocytosis

    International Nuclear Information System (INIS)

    Pernet, Olivier; Pohl, Christine; Ainouze, Michelle; Kweder, Hasan; Buckland, Robin

    2009-01-01

    Nipah virus (NiV) is a zoonotic biosafety level 4 paramyxovirus that emerged recently in Asia with high mortality in man. NiV is a member, with Hendra virus (HeV), of the Henipavirus genus in the Paramyxoviridae family. Although NiV entry, like that of other paramyxoviruses, is believed to occur via pH-independent fusion with the host cell's plasma membrane we present evidence that entry can occur by an endocytic pathway. The NiV receptor ephrinB2 has receptor kinase activity and we find that ephrinB2's cytoplasmic domain is required for entry but is dispensable for post-entry viral spread. The mutation of a single tyrosine residue (Y304F) in ephrinB2's cytoplasmic tail abrogates NiV entry. Moreover, our results show that NiV entry is inhibited by constructions and drugs specific for the endocytic pathway of macropinocytosis. Our findings could potentially permit the rapid development of novel low-cost antiviral treatments not only for NiV but also HeV.

  17. Nuclear science references coding manual

    International Nuclear Information System (INIS)

    Ramavataram, S.; Dunford, C.L.

    1996-08-01

    This manual is intended as a guide to Nuclear Science References (NSR) compilers. The basic conventions followed at the National Nuclear Data Center (NNDC), which are compatible with the maintenance and updating of and retrieval from the Nuclear Science References (NSR) file, are outlined. In Section H, the structure of the NSR file such as the valid record identifiers, record contents, text fields as well as the major TOPICS for which are prepared are enumerated. Relevant comments regarding a new entry into the NSR file, assignment of , generation of and linkage characteristics are also given in Section II. In Section III, a brief definition of the Keyword abstract is given followed by specific examples; for each TOPIC, the criteria for inclusion of an article as an entry into the NSR file as well as coding procedures are described. Authors preparing Keyword abstracts either to be published in a Journal (e.g., Nucl. Phys. A) or to be sent directly to NNDC (e.g., Phys. Rev. C) should follow the illustrations in Section III. The scope of the literature covered at the NNDC, the categorization into Primary and Secondary sources, etc., is discussed in Section IV. Useful information regarding permitted character sets, recommended abbreviations, etc., is given under Section V as Appendices

  18. Proposed nuclear weapons nonproliferation policy concerning foreign research reactor spent nuclear fuel. Summary

    International Nuclear Information System (INIS)

    1995-03-01

    The United States Department of Energy and United States Department of State are jointly proposing to adopt a policy to manage spent nuclear fuel from foreign research reactors. Only spent nuclear fuel containing uranium enriched in the United States would be covered by the proposed policy. The purpose of the proposed policy is to promote U.S. nuclear weapons nonproliferation policy objectives, specifically by seeking to reduce highly-enriched uranium from civilian commerce. This is a summary of the Draft Environmental Impact Statement. Environmental effects and policy considerations of three Management Alternative approaches for implementation of the proposed policy are assessed. The three Management Alternatives analyzed are: (1) acceptance and management of the spent nuclear fuel by the Department of Energy in the United States, (2) management of the spent nuclear fuel at one or more foreign facilities (under conditions that satisfy United States nuclear weapons nonproliferation policy objectives), and (3) a combination of components of Management Alternatives 1 and 2 (Hybrid Alternative). A No Action Alternative is also analyzed. For each Management Alternative, there are a number of alternatives for its implementation. For Management Alternative 1, this document addresses the environmental effects of various implementation alternatives such as varied policy durations, management of various quantities of spent nuclear fuel, and differing financing arrangements. Environmental impacts at various potential ports of entry, along truck and rail transportation routes, at candidate management sites, and for alternate storage technologies are also examined. For Management Alternative 2, this document addresses two subalternatives: (1) assisting foreign nations with storage; and (2) assisting foreign nations with reprocessing of the spent nuclear fuel

  19. Progress report on nuclear science and technology in China (Vol.3). Proceedings of academic annual meeting of China Nuclear Society in 2013, No.3--nuclear power sub-volume (Pt.2)

    International Nuclear Information System (INIS)

    2014-05-01

    Progress report on nuclear science and technology in China (Vol. 3) includes 86 articles which are communicated on the third national academic annual meeting of China Nuclear Society. There are 10 books totally. This is the third one, the content is about nuclear power sub-volume (Pt.2)

  20. MARKET ENTRY STRATEGIES TO EMERGING MARKETS: A CONCEPTUAL MODEL OF TURNKEY PROJECT DEVELOPMENT

    Directory of Open Access Journals (Sweden)

    Bistra Vassileva

    2016-11-01

    Full Text Available The main purpose of the paper is to analyse the international market entry strategies in the light of globalisation processes and to propose a conceptual model of turnkey projects as market entry mode. The specific research objectives are as follows: 1. to develop an integrated framework of the turnkey marketing process as a conceptual model; 2. to analyse BRICS countries as potential host countries for turnkey projects implementation; 3. to assess potential implications of proposed conceptual model for global market entry decisions.

  1. Exporting Complex Digital Products: Motives and Entry Modes

    DEFF Research Database (Denmark)

    Rask, Morten

    2005-01-01

    When the product is digital, it will most often be distributed directly to the customer through the Internet, and therefore the entry modes, considered in this paper, are different flavors of the entry mode called direct export: Virtual export channel are generally understood as the entry mode fo...... for digital product providers. However other types of entry modes like what wee call direct digital export with F2F-sales, direct digital export with F2F-support and virtual sales subsidiary are entry modes that respond to a higher degree of pre- and after-sales complexity....

  2. PDBsum: Structural summaries of PDB entries

    OpenAIRE

    Laskowski, Roman A.; Jabłońska, Jagoda; Pravda, Lukáš; Vařeková, Radka Svobodová; Thornton, Janet M.

    2017-01-01

    Abstract PDBsum is a web server providing structural information on the entries in the Protein Data Bank (PDB). The analyses are primarily image‐based and include protein secondary structure, protein‐ligand and protein‐DNA interactions, PROCHECK analyses of structural quality, and many others. The 3D structures can be viewed interactively in RasMol, PyMOL, and a JavaScript viewer called 3Dmol.js. Users can upload their own PDB files and obtain a set of password‐protected PDBsum analyses for e...

  3. Dictionary of nuclear engineering. English-German-French-Russian. Kerntechnik. Englisch-Deutsch-Franzoesisch-Russisch

    Energy Technology Data Exchange (ETDEWEB)

    Sube, R

    1985-01-01

    This volume contains nearly 30,000 entries from all branches of nuclear engineering excluding biological aspects, health physics, and isotope applications in medicine and agriculture. The terminology used in the International Nuclear Information System (INIS) of the IAEA has been completely taken into account. Each English entry is numbered and is followed by corresponding terms in the other languages. Alphabetical indexes of the German, French, and Russian terms are included. At the end of the volume, there is a section devoted to the transliteration of place-names between the English names and their Cyrillic equivalents, and vice versa.

  4. Isolation and characterization of reverse transcriptase fragments of LTR retrotransposons from the genome of Chenopodium quinoa (Amaranthaceae).

    Science.gov (United States)

    Kolano, Bozena; Bednara, Edyta; Weiss-Schneeweiss, Hanna

    2013-10-01

    High heterogeneity was observed among conserved domains of reverse transcriptase ( rt ) isolated from quinoa. Only one Ty1- copia rt was highly amplified. Reverse transcriptase sequences were located predominantly in pericentromeric region of quinoa chromosomes. The heterogeneity, genomic abundance, and chromosomal distribution of reverse transcriptase (rt)-coding fragments of Ty1-copia and Ty3-gypsy long terminal repeat retrotransposons were analyzed in the Chenopodium quinoa genome. Conserved domains of the rt gene were amplified and characterized using degenerate oligonucleotide primer pairs. Sequence analyses indicated that half of Ty1-copia rt (51 %) and 39 % of Ty3-gypsy rt fragments contained intact reading frames. High heterogeneity among rt sequences was observed for both Ty1-copia and Ty3-gypsy rt amplicons, with Ty1-copia more heterogeneous than Ty3-gypsy. Most of the isolated rt fragments were present in quinoa genome in low copy numbers, with only one highly amplified Ty1-copia rt sequence family. The gypsy-like RNase H fragments co-amplified with Ty1-copia-degenerate primers were shown to be highly amplified in the quinoa genome indicating either higher abundance of some gypsy families of which rt domains could not be amplified, or independent evolution of this gypsy-region in quinoa. Both Ty1-copia and Ty3-gypsy retrotransposons were preferentially located in pericentromeric heterochromatin of quinoa chromosomes. Phylogenetic analyses of newly amplified rt fragments together with well-characterized retrotransposon families from other organisms allowed identification of major lineages of retroelements in the genome of quinoa and provided preliminary insight into their evolutionary dynamics.

  5. Challenges in ensuring radiological safety and nuclear forensic for malicious acts involving nuclear and other radioactive material

    International Nuclear Information System (INIS)

    Sharma, Ranjit; Chatterjee, M.K.; Singh, Rajvir; Pradeepkumar, K.S.

    2010-01-01

    Nuclear and other radioactive materials may get smuggled into the country aimed at malicious acts. Radioactive material detected accidentally or during inspection at the entry points/national borders may indicate illicit trafficking for the purpose of nuclear/radiological terrorism. As country requires prevention and preparedness for response to these malicious acts, nuclear forensic techniques are to be developed incorporating radiological safety aspects. Nuclear forensics helps in determining the origin, intended use, legal owner and the smuggled route etc. by using fingerprinting as well as comparison with reference data. The suggested sequence of methods for analysis of radioactive material/samples will be radiological assessment, physical characterization, traditional forensic analysis, isotope analysis along with elemental/chemical analysis

  6. MANIA (276-3/4/5). Nuclear analysis

    International Nuclear Information System (INIS)

    Sciolla, C.M.

    1993-11-01

    This report contains the results of the nuclear calculations performed for the MANIA-276 experiment, sample holders 3, 4 and 5. The codes MICROFLUX-2, GAM, HFR-TEDDI and ORIGEN-S have been used for this analysis. Nuclear constants, dpa, reactivity effect and activity of the samples and of the structural materials have been calculated. The results are given in the tables and appendices of the present report. (orig.)

  7. Decision in Principle of the Council of State on the organisation of nuclear waste management

    International Nuclear Information System (INIS)

    1978-01-01

    This Decision, which came into force on the date of its publication, contains general guidelines to be followed for nuclear waste management in nuclear facilities until the entry into force of special legislation on the organisational and economic aspects of such management. It provides in particular that radioactive waste 'producers' will bear financial responsibility for the waste they produce and must collect funds for waste management from the time their installation operates, while ensuring that the real value of these funds is maintained. During the period preceding the entry into force of the above-mentioned legislation, the terms of the Decision will be included in all permits for nuclear facilities. (NEA) [fr

  8. Radon entry into buildings: Effects of atmospheric pressure fluctuations and building structural factors

    Energy Technology Data Exchange (ETDEWEB)

    Robinson, Allen Lantham [Univ. of California, Berkeley, CA (United States). Dept. of Mechanical Engineering

    1996-05-01

    An improved understanding of the factors that control radon entry into buildings is needed in order to reduce the public health risks caused by exposure to indoor radon. This dissertation examines three issues associated with radon entry into buildings: (1) the influence of a subslab gravel layer and the size of the openings between the soil and the building interior on radon entry; (2) the effect of atmospheric pressure fluctuations on radon entry; and (3) the development and validation of mathematical models which simulate radon and soil-gas entry into houses. Experiments were conducted using two experimental basements to examine the influence of a subslab gravel layer on advective radon entry driven by steady indoor-outdoor pressure differences. These basement structures are identical except that in one the floor slab lies directly on native soil whereas in the other the slab lies on a high-permeability gravel layer. The measurements indicate that a high permeability subslab gravel layer increases the advective radon entry rate into the structure by as much as a factor of 30. The magnitude of the enhancement caused by the subslab gravel layer depends on the area of the openings in the structure floor; the smaller the area of these openings the larger the enhancement in the radon entry rate caused by the subslab gravel layer. A three-dimensional, finite-difference model correctly predicts the effect of a subslab gravel layer and open area configuration on advective radon entry driven by steady indoor-outdoor pressure differences; however, the model underpredicts the absolute entry rate into each structure by a factor of 1.5.

  9. Radon entry into buildings: Effects of atmospheric pressure fluctuations and building structural factors

    International Nuclear Information System (INIS)

    Robinson, A.L.

    1996-05-01

    An improved understanding of the factors that control radon entry into buildings is needed in order to reduce the public health risks caused by exposure to indoor radon. This dissertation examines three issues associated with radon entry into buildings: (1) the influence of a subslab gravel layer and the size of the openings between the soil and the building interior on radon entry; (2) the effect of atmospheric pressure fluctuations on radon entry; and (3) the development and validation of mathematical models which simulate radon and soil-gas entry into houses. Experiments were conducted using two experimental basements to examine the influence of a subslab gravel layer on advective radon entry driven by steady indoor-outdoor pressure differences. These basement structures are identical except that in one the floor slab lies directly on native soil whereas in the other the slab lies on a high-permeability gravel layer. The measurements indicate that a high permeability subslab gravel layer increases the advective radon entry rate into the structure by as much as a factor of 30. The magnitude of the enhancement caused by the subslab gravel layer depends on the area of the openings in the structure floor; the smaller the area of these openings the larger the enhancement in the radon entry rate caused by the subslab gravel layer. A three-dimensional, finite-difference model correctly predicts the effect of a subslab gravel layer and open area configuration on advective radon entry driven by steady indoor-outdoor pressure differences; however, the model underpredicts the absolute entry rate into each structure by a factor of 1.5

  10. Physical security workshop summary: entry control

    International Nuclear Information System (INIS)

    Eaton, M.J.

    1982-01-01

    Entry control hardware has been used extensively in the past to assist security forces in separating the authorized from the unauthorized at the plant perimeter. As more attention is being focused on the insider threat, these entry control elements are being used to extend the security inspectors' presence into the plant by compartmentalizing access and monitoring vital components. This paper summarizes the experiences expressed by the participants at the March 16 to 19, 1982 INMM Physical Protection Workshop in utilizing access control and contraband detection hardware for plant wide entry control applications

  11. Exclusive Dealing and Entry

    OpenAIRE

    João Leão

    2008-01-01

    This paper examines the use of exclusive dealing agreements to prevent the entry of rival firms. An exclusive dealing agreement is a contract between a buyer and a seller where the buyer commits to buy a good exclusively from the seller. One main concern of the literature is to explain how an incumbent seller is able to persuade the buyers to sign an exclusive dealing agreement that deters the entry of a more efficient rival seller. We propose a new explanation when the buyers are downstream ...

  12. Entry regulations, welfare and determinants of market structure

    OpenAIRE

    Maican, Florin; Orth, Matilda

    2015-01-01

    We use a dynamic oligopoly model of entry and exit with store-type differentiation to evaluate how entry regulations affect profitability, market structure and welfare. Based on unique data for all retail food stores in Sweden, we estimate demand, recover variable profits, and estimate entry costs and fixed costs by store type. Counterfactual policy experiments show that welfare increases when competition is enhanced by lower entry costs. Protecting small stores by imposing licensing fees on ...

  13. 3. Mexican school of nuclear physics; 3. Escuela Mexicana de Fisica Nuclear

    Energy Technology Data Exchange (ETDEWEB)

    Chavez L, E R [Instituto de Fisica, UNAM, 04510 Mexico D.F. (Mexico); Hess, P O [Instituto de Ciencias Nucleares, UNAM, 04510 Mexico D.F. (Mexico); Martinez Q, E [Instituto Nacional de Investigaciones Nucleares, A.P. 18-1027, 11801 Mexico D.F. (Mexico)

    2002-07-01

    The III Mexican School of Nuclear Physics which is directed to those post graduate in Sciences and those of last semesters students of the Physics career or some adjacent career was organized by the Nuclear Physics Division of the Mexican Physics Society, carrying out at November 18-29, 2002 in the installations of the Institute of Physics and the Institute of Nuclear Sciences both in the UNAM, and the National Institute of Nuclear Research (ININ). In this as well as the last version its were offered 17 courses, 9 of them including laboratory practices and the rest were of theoretical character only. This book treats about the following themes: Nuclear physics, Electrostatic accelerators, Cyclotrons, Thermonuclear reactions, Surface barrier detectors, Radiation detection, Neutron detection, Bonner sphere spectrometers, Radiation protection, Biological radiation effects, Particle kinematics, Nucleosynthesis, Plastics, Muons, Quadrupoles, Harmonic oscillators, Quantum mechanics among many other matters. (Author)

  14. Foreign Entry and Heterogeneous Growth of Firms

    DEFF Research Database (Denmark)

    Deng, Paul Duo; Jefferson, Gary H.

    We adopt the framework of Schumpeterian creative destruction formalized by Aghion et al. (2009) to analyze the impact of foreign entry on the productivity growth of domestic firms. In the face of foreign entry, domestic firms exhibit heterogeneous patterns of growth depending on their technologic...... manufacturing. Our empirical results confirm that foreign entry indeed generates strong heterogeneous growth patterns among domestic firms.......We adopt the framework of Schumpeterian creative destruction formalized by Aghion et al. (2009) to analyze the impact of foreign entry on the productivity growth of domestic firms. In the face of foreign entry, domestic firms exhibit heterogeneous patterns of growth depending on their technological...... distance from foreign firms. Domestic firms with smaller technological distance from their foreign counterparts tend to experience faster productivity growth, while firms with larger technological distance tend to lag further behind. We test this hypothesis using a unique firm-level data of Chinese...

  15. Proceedings of NUCLEAR 2013 the 6th annual international conference on sustainable development through nuclear research and education. Part 1/3

    International Nuclear Information System (INIS)

    Constantin, Marin; Turcu, Ilie

    2013-01-01

    The proceedings of the NUCLEAR 2013 international conference on sustainable development through nuclear research and education held at INR-Pitesti on May, 2-24 2013 contain 79 communications presented in two plenary sessions (6 and 8 talks, respectively) and three sections addressing the themes of Nuclear energy, Environmental protection, and Sustainable development . In turn these sections are addressing the following items: Section 1.1 - Nuclear Technology and Materials (33 papers); Section 1.2 Nuclear Safety and Sever Accidents (16 papers); 1.3 Nuclear Reactors and Gen IV (20 papers); 2.1 Radioactive Waste Management (13 papers); 2.2 Radioprotection and 2.3 Air, Water, Soil Protection (17 papers); 3.1 Polices and Strategies in Nuclear Research (0 papers); 3.2 International Partnership for a Sustainable Development (2 papers); 3.3 Education, Continuous Formation and Knowledge Transfer (1 papers). These papers are presented as abstracts in 'Nuclear 2013 - Book of Abstracts', separately processed

  16. Proceedings of NUCLEAR 2015 the 8th annual international conference on sustainable development through nuclear research and education. Part 1/3

    International Nuclear Information System (INIS)

    Constantin, Marin; Turcu, Ilie

    2015-01-01

    The proceedings of the NUCLEAR 2015 the 8"t"h annual international conference on sustainable development through nuclear research and education. Part 1/3 held at INR-Pitesti on May, 27-29 contain 62 communications presented in two plenary sessions and three sections addressing the themes of Nuclear energy, Environmental protection and Sustainable development. In turn these sections are addressing the following items: Section 1.1 Nuclear safety and severe accidents (7 papers); Section 1.2 Nuclear reactors and gen. IV (5 papers); Section 1.3 Nuclear technology and materials (19 papers); Section 2.1 Radioprotection & air, water and soil protection (1 paper); Section 2.2 Radioactive waste management (9 papers); Section 3.1 policies and strategies in nuclear research (1 paper); Section 3.2 Education, training and knowledge management (16 papers); Section 3.3 International partnership for a sustainable development (4 papers). These papers are presented as abstracts in 'Nuclear 2015 - Book of Abstracts', separately processed

  17. Energy Information Data Base: corporate author entries

    International Nuclear Information System (INIS)

    1980-06-01

    One of the controls for information entered into the data bases created and maintained by the DOE Technical Information Center is the standardized name for the corporate entity or the corporate author. The purpose of Energy Information Data Base: Corporate Author Entries is to provide a means for the consistent citing of the names of organizations in bibliographic records. These entries serve as guides for users of the DOE/RECON computerized data bases who want to locate information originating in particular organizations. The entries in this revision include the corporate entries used in report bibliographic citations since 1973 and list approximately 28,000 corporate sources

  18. 3D visualization based customer experiences of nuclear plant control room

    International Nuclear Information System (INIS)

    Sun Tienlung; Chou Chinmei; Hung Tamin; Cheng Tsungchieh; Yang Chihwei; Yang Lichen

    2011-01-01

    This paper employs virtual reality (VR) technology to develop an interactive virtual nuclear plant control room in which the general public could easily walk into the 'red zone' and play with the control buttons. The VR-based approach allows deeper and richer customer experiences that the real nuclear plant control room could not offer. When people know more about the serious process control procedures enforced in the nuclear plant control room, they will appropriate more about the safety efforts imposed by the nuclear plant and become more comfortable about the nuclear plant. The virtual nuclear plant control room is built using a 3D game development tool called Unity3D. The 3D scene is connected to a nuclear plant simulation system through Windows API programs. To evaluate the usability of the virtual control room, an experiment will be conducted to see how much 'immersion' the users could feel when they played with the virtual control room. (author)

  19. Cardiac re-entry dynamics and self-termination in DT-MRI based model of Human Foetal Heart

    Science.gov (United States)

    Biktasheva, Irina V.; Anderson, Richard A.; Holden, Arun V.; Pervolaraki, Eleftheria; Wen, Fen Cai

    2018-02-01

    The effect of human foetal heart geometry and anisotropy on anatomy induced drift and self-termination of cardiac re-entry is studied here in MRI based 2D slice and 3D whole heart computer simulations. Isotropic and anisotropic models of 20 weeks of gestational age human foetal heart obtained from 100μm voxel diffusion tensor MRI data sets were used in the computer simulations. The fiber orientation angles of the heart were obtained from the orientation of the DT-MRI primary eigenvectors. In a spatially homogeneous electrophysiological monodomain model with the DT-MRI based heart geometries, cardiac re-entry was initiated at a prescribed location in a 2D slice, and in the 3D whole heart anatomy models. Excitation was described by simplified FitzHugh-Nagumo kinetics. In a slice of the heart, with propagation velocity twice as fast along the fibres than across the fibers, DT-MRI based fiber anisotropy changes the re-entry dynamics from pinned to an anatomical re-entry. In the 3D whole heart models, the fiber anisotropy changes cardiac re-entry dynamics from a persistent re-entry to the re-entry self-termination. The self-termination time depends on the re-entry’s initial position. In all the simulations with the DT-MRI based cardiac geometry, the anisotropy of the myocardial tissue shortens the time to re-entry self-termination several folds. The numerical simulations depend on the validity of the DT-MRI data set used. The ventricular wall showed the characteristic transmural rotation of the helix angle of the developed mammalian heart, while the fiber orientation in the atria was irregular.

  20. Nuclear security from cradle to grave

    International Nuclear Information System (INIS)

    Raja Adnan, Raja Abdul Aziz

    2016-01-01

    On 8 May 2016, the Amendment to the Convention on the Physical Protection of Nuclear Material (CPPNM) finally entered into force, almost eleven years after its adoption. The world will be a more secure place as a result of the commitments that States party to the Amendment have made. The Amendment establishes legally binding commitments for countries to protect nuclear facilities as well as nuclear material in domestic use, storage and transport. Under the Amendment, countries are required to establish appropriate physical protection regimes for nuclear material. They also take on new obligations to share information on sabotage, including on credible threats of sabotage. The entry into force of the Amendment demonstrates the international community’s resolve to act together to strengthen nuclear security globally. It also helps reduce the risk of an attack involving nuclear material, which could have catastrophic consequences.

  1. Ebola virus host cell entry.

    Science.gov (United States)

    Sakurai, Yasuteru

    2015-01-01

    Ebola virus is an enveloped virus with filamentous structure and causes a severe hemorrhagic fever in human and nonhuman primates. Host cell entry is the first essential step in the viral life cycle, which has been extensively studied as one of the therapeutic targets. A virus factor of cell entry is a surface glycoprotein (GP), which is an only essential viral protein in the step, as well as the unique particle structure. The virus also interacts with a lot of host factors to successfully enter host cells. Ebola virus at first binds to cell surface proteins and internalizes into cells, followed by trafficking through endosomal vesicles to intracellular acidic compartments. There, host proteases process GPs, which can interact with an intracellular receptor. Then, under an appropriate circumstance, viral and endosomal membranes are fused, which is enhanced by major structural changes of GPs, to complete host cell entry. Recently the basic research of Ebola virus infection mechanism has markedly progressed, largely contributed by identification of host factors and detailed structural analyses of GPs. This article highlights the mechanism of Ebola virus host cell entry, including recent findings.

  2. Nuclear security of Cuba’s medical facilities

    International Nuclear Information System (INIS)

    Dahlstrom, Danielle

    2016-01-01

    Cuba is a leading hub for medical research and cancer treatment in Latin America and the Caribbean. Physical protection is installed at radiotherapy facilities to detect entry of and delay access to an intruder. This minimizes the likelihood of unauthorized access and maximizes nuclear security.

  3. Simulations. 3D nuclear reactions; Simulations. Reactions nucleaires en 3D

    Energy Technology Data Exchange (ETDEWEB)

    Deleurence, Guillaume

    2012-05-15

    At CEA Saclay, the research programme for the forth generation of nuclear reactors, named 'Astrid' (Advanced Sodium Technological Reactor for Industrial Demonstration) of the Direction of nuclear energy (DEN), uses 2D or 3D movies and a 16 m{sup 2} image wall for the display of simulation results. This digest paper focusses on the technological means used for the simulations: the Curie supercomputer designed by Bull and the images display device. (J.S.)

  4. Free Entry and Social Inefficiency under Co-opetition

    OpenAIRE

    Hattori, Keisuke; Yoshikawa, Takeshi

    2013-01-01

    We investigate the social desirability of free entry in the co-opetition model in which firms compete in a homogeneous product market while sharing common property resources that affect market size or consumers' willingness to pay for products. We show that free entry leads to socially excessive or insufficient entry into the market in the case of non-commitment co-opetition, depending on the magnitude of "business stealing" and "common property" effects of entry. On the other hand, in the ca...

  5. Entry control system for large populations

    International Nuclear Information System (INIS)

    Merillat, P.D.

    1982-01-01

    An Entry Control System has been developed which is appropriate for use at an installation with a large population requiring access over a large area. This is accomplished by centralizing the data base management and enrollment functions and decentralizing the guard-assisted, positive personnel identification and access functions. Current information pertaining to all enrollees is maintained through user-friendly enrollment stations. These stations may be used to enroll individuals, alter their area access authorizations, change expiration dates, and other similar functions. An audit trail of data base alterations is provided to the System Manager. Decentrailized systems exist at each area to which access is controlled. The central system provides these systems with the necessary entry control information to allow them to operate microprocessor-driven entry control devices. The system is comprised of commercially available entry control components and is structured such that it will be able to incorporate improved devices as technology porogresses. Currently, access is granted to individuals who possess a valid credential, have current access authorization, can supply a memorized personal identification number, and whose physical hand dimensions match their profile obtained during enrollment. The entry control devices report misuses as security violations to a Guard Alarm Display and Assessment System

  6. The SUN protein Mps3 is required for spindle pole body insertion into the nuclear membrane and nuclear envelope homeostasis.

    Directory of Open Access Journals (Sweden)

    Jennifer M Friederichs

    2011-11-01

    Full Text Available The budding yeast spindle pole body (SPB is anchored in the nuclear envelope so that it can simultaneously nucleate both nuclear and cytoplasmic microtubules. During SPB duplication, the newly formed SPB is inserted into the nuclear membrane. The mechanism of SPB insertion is poorly understood but likely involves the action of integral membrane proteins to mediate changes in the nuclear envelope itself, such as fusion of the inner and outer nuclear membranes. Analysis of the functional domains of the budding yeast SUN protein and SPB component Mps3 revealed that most regions are not essential for growth or SPB duplication under wild-type conditions. However, a novel dominant allele in the P-loop region, MPS3-G186K, displays defects in multiple steps in SPB duplication, including SPB insertion, indicating a previously unknown role for Mps3 in this step of SPB assembly. Characterization of the MPS3-G186K mutant by electron microscopy revealed severe over-proliferation of the inner nuclear membrane, which could be rescued by altering the characteristics of the nuclear envelope using both chemical and genetic methods. Lipid profiling revealed that cells lacking MPS3 contain abnormal amounts of certain types of polar and neutral lipids, and deletion or mutation of MPS3 can suppress growth defects associated with inhibition of sterol biosynthesis, suggesting that Mps3 directly affects lipid homeostasis. Therefore, we propose that Mps3 facilitates insertion of SPBs in the nuclear membrane by modulating nuclear envelope composition.

  7. Orthopoxvirus species and strain differences in cell entry

    Energy Technology Data Exchange (ETDEWEB)

    Bengali, Zain; Satheshkumar, P.S. [Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892-3210 (United States); Moss, Bernard, E-mail: bmoss@nih.gov [Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892-3210 (United States)

    2012-11-25

    Vaccinia virus (VACV) enters cells by a low pH endosomal route or by direct fusion with the plasma membrane. We previously found differences in entry properties of several VACV strains: entry of WR was enhanced by low pH, reduced by bafilomycin A1 and relatively unaffected by heparin, whereas entry of IHD-J, Copenhagen and Elstree were oppositely affected. Since binding and entry modes may have been selected by specific conditions of in vitro propagation, we now examined the properties of three distinct, recently isolated cowpox viruses and a monkeypox virus as well as additional VACV and cowpox virus strains. The recent isolates were more similar to WR than to other VACV strains, underscoring the biological importance of endosomal entry by orthopoxviruses. Sequence comparisons, gene deletions and gene swapping experiments indicated that viral determinants, other than or in addition to the A26 and A25 'fusion-suppressor' proteins, impact entry properties.

  8. 40 CFR 170.112 - Entry restrictions.

    Science.gov (United States)

    2010-07-01

    ...-entry interval applies, including, but not limited to, soil, water, air, or surfaces of plants; and (2...-entry activity, the agricultural employer shall provide a decontamination site in accordance with § 170... running water for routine and emergency decontamination and mechanical devices that would reduce the...

  9. An Analysis of the Feasible Entry Mode for Tottenham Hotspur’s Entry into China

    OpenAIRE

    Zhang, Lei

    2013-01-01

    An increasing number of European football clubs has developed global strategies with an emphasis on the Chinese market, which seems to be a shortcut to their global success. In order to achieve an effective expansion, the selection of appropriate entry modes should be conducted cautiously. This paper aims to select feasible entry modes for Tottenham Hotspur F.C., an emerging power in the Premier League, to enter into the Chinese market. A framework that combines different theories based ...

  10. Summary analysis of the Gemini entry aerodynamics

    Science.gov (United States)

    Whitnah, A. M.; Howes, D. B.

    1972-01-01

    The aerodynamic data that were derived in 1967 from the analysis of flight-generated data for the Gemini entry module are presented. These data represent the aerodynamic characteristics exhibited by the vehicle during the entry portion of Gemini 2, 3, 5, 8, 10, 11, and 12 missions. For the Gemini, 5, 8, 10, 11, and 12 missions, the flight-generated lift-to-drag ratios and corresponding angles of attack are compared with the wind tunnel data. These comparisons show that the flight generated lift-to-drag ratios are consistently lower than were anticipated from the tunnel data. Numerous data uncertainties are cited that provide an insight into the problems that are related to an analysis of flight data developed from instrumentation systems, the primary functions of which are other than the evaluation of flight aerodynamic performance.

  11. Dependency of radon entry on pressure difference

    International Nuclear Information System (INIS)

    Kokotti, H.; Kalliokoski, P.

    1992-01-01

    Radon levels, ventilation rate and pressure differences were monitored continuously in four apartment houses with different ventilation systems. Two of them were ventilated by mechanical exhaust, one by mechanical supply and exhaust, and one by natural ventilation. The two-storey houses were constructed from concrete elements on a slab and located on a gravel esker. It was surprising to find that increasing the ventilation rate increased levels of radon in the apartments. Increased ventilation caused increased outdoor-indoor pressure difference, which in turn increased the entry rate of radon and counteracted the diluting effect of ventilation. The increase was significant when the outdoor-indoor pressure difference exceeded 5 Pa. Especially in the houses with mechanical exhaust ventilation the pressure difference was the most important factor of radon entry rate, and contributed up to several hundred Bq m -3 h -1 . (Author)

  12. Sun-mediated mechanical LINC between nucleus and cytoskeleton regulates βcatenin nuclear access.

    Science.gov (United States)

    Uzer, Gunes; Bas, Guniz; Sen, Buer; Xie, Zhihui; Birks, Scott; Olcum, Melis; McGrath, Cody; Styner, Maya; Rubin, Janet

    2018-06-06

    βcatenin acts as a primary intracellular signal transducer for mechanical and Wnt signaling pathways to control cell function and fate. Regulation of βcatenin in the cytoplasm has been well studied but βcatenin nuclear trafficking and function remains unclear. In a previous study we showed that, in mesenchymal stem cells (MSC), mechanical blockade of adipogenesis relied on inhibition of βcatenin destruction complex element GSK3β (glycogen synthase kinase 3β) to increase nuclear βcatenin as well as the function of Linker of Cytoskeleton and Nucleoskeleton (LINC) complexes, suggesting that these two mechanisms may be linked. Here we show that shortly after inactivation of GSK3β due to either low intensity vibration (LIV), substrate strain or pharmacologic inhibition, βcatenin associates with the nucleoskeleton, defined as the insoluble nuclear fraction that provides structure to the integrated nuclear envelope, nuclear lamina and chromatin. Co-depleting LINC elements Sun-1 and Sun-2 interfered with both nucleoskeletal association and nuclear entry of βcatenin, resulting in decreased nuclear βcatenin levels. Our findings reveal that the insoluble structural nucleoskeleton actively participates in βcatenin dynamics. As the cytoskeleton transmits applied mechanical force to the nuclear surface to influence the nucleoskeleton and its LINC mediated interaction, our results suggest a pathway by which LINC mediated connectivity may play a role in signaling pathways that depend on nuclear access of βcatenin. Copyright © 2018 Elsevier Ltd. All rights reserved.

  13. Analytic Development of a Reference Profile for the First Entry in a Skip Atmospheric Entry

    Science.gov (United States)

    Garcia-Llama, Eduardo

    2010-01-01

    This note shows that a feasible reference drag profile for the first entry portion of a skip entry can be generated as a polynomial expression of the velocity. The coefficients of that polynomial are found through the resolution of a system composed of m + 1 equations, where m is the degree of the drag polynomial. It has been shown that a minimum of five equations (m = 4) are required to establish the range and the initial and final conditions on velocity and flight path angle. It has been shown that at least one constraint on the trajectory can be imposed through the addition of one extra equation in the system, which must be accompanied by the increase in the degree of the drag polynomial. In order to simplify the resolution of the system of equations, the drag was considered as being a probability density function of the velocity, with the velocity as a distribution function of the drag. Combining this notion with the introduction of empirically derived constants, it has been shown that the system of equations required to generate the drag profile can be successfully reduced to a system of linear algebraic equations. For completeness, the resulting drag profiles have been flown using the feedback linearization method of differential geometric control as a guidance law with the error dynamics of a second order homogeneous equation in the form of a damped oscillator. Satisfactory results were achieved when the gains in the error dynamics were changed at a certain point along the trajectory that is dependent on the velocity and the curvature of the drag as a function of the velocity. Future work should study the capacity to update the drag profile in flight when dispersions are introduced. Also, future studies should attempt to link the first entry, as presented and controlled in this note, with a more standard control concept for the second entry, such as the Apollo entry guidance, to try to assess the overall skip entry performance. A guidance law that includes

  14. Draft Environmental Impact Statement on a proposed nuclear weapons nonproliferation policy concerning foreign research reactor spent nuclear fuel. Volume 1

    International Nuclear Information System (INIS)

    1995-03-01

    The United States Department of Energy and United States Department of State are jointly proposing to adopt a policy to manage spent nuclear fuel from foreign research reactors. Only spent nuclear fuel containing uranium enriched in the United States would be covered by the proposed policy. The purpose of the proposed policy is to promote U.S. nuclear weapons nonproliferation policy objectives, specifically by seeking to reduce highly-enriched uranium from civilian commerce. Environmental effects and policy considerations of three Management Alternative approaches for implementation of the proposed policy are assessed. The three Management Alternatives analyzed are: (1) acceptance and management of the spent nuclear fuel by the Department of Energy in the United States, (2) management of the spent nuclear fuel at one or more foreign facilities (under conditions that satisfy United States nuclear weapons nonproliferation policy objectives), and (3) a combination of components of Management Alternatives 1 and 2 (Hybrid Alternative). A No Action Alternative is also analyzed. For each Management Alternative, there are a number of alternatives for its implementation. For Management Alternative 1, this document addresses the environmental effects of various implementation alternatives such as varied policy durations, management of various quantities of spent nuclear fuel, and differing financing arrangements. Environmental impacts at various potential ports of entry, along truck and rail transportation routes, at candidate management sites, and for alternate storage technologies are also examined. For Management Alternative 2, this document addresses two subalternatives: (1) assisting foreign nations with storage; and (2) assisting foreign nations with reprocessing of the spent nuclear fuel. Management Alternative 3 analyzes a hybrid alternative. This document is Vol. 1 of 2 plus summary volume

  15. Protocol Additional to the Agreement between the Republic of Iraq and the International Atomic Energy Agency for the Application of Safeguards in Connection with the Treaty on the Non-Proliferation of Nuclear Weapons. Entry into Force

    International Nuclear Information System (INIS)

    2012-01-01

    Pursuant to Article 17 of the Protocol Additional to the Agreement between the Republic of Iraq and the International Atomic Energy Agency for the Application of Safeguards in Connection with the Treaty on the Non-Proliferation of Nuclear Weapons (the Additional Protocol), the Additional Protocol, which had been applied provisionally from 17 February 2010, entered into force on 10 October 2012, the date upon which the Agency received written notification from Iraq that Iraq's statutory and/or constitutional requirements for entry into force had been met [es

  16. Enhancing Nuclear Training with 3D Visualization

    International Nuclear Information System (INIS)

    Gagnon, V.; Gagnon, B.

    2016-01-01

    Full text: While the nuclear power industry is trying to reinforce its safety and regain public support post-Fukushima, it is also faced with a very real challenge that affects its day-to-day activities: a rapidly aging workforce. Statistics show that close to 40% of the current nuclear power industry workforce will retire within the next five years. For newcomer countries, the challenge is even greater, having to develop a completely new workforce. The workforce replacement effort introduces nuclear newcomers of a new generation with different backgrounds and affinities. Major lifestyle differences between the two generations of workers result, amongst other things, in different learning habits and needs for this new breed of learners. Interactivity, high visual content and quick access to information are now necessary to achieve a high level of retention. To enhance existing training programmes or to support the establishment of new training programmes for newcomer countries, L-3 MAPPS has devised learning tools to enhance these training programmes focused on the “Practice-by-Doing” principle. L-3 MAPPS has coupled 3D computer visualization with high-fidelity simulation to bring real-time, simulation-driven animated components and systems allowing immersive and participatory, individual or classroom learning. (author

  17. Entry rates and recycling of glucose in buffalo calves fed on urea molasses liquid diet

    International Nuclear Information System (INIS)

    Varma, Arun; Singh, U.B.; Verma, D.N.; Ranjhan, S.K.

    1974-01-01

    Entry rates of glucose have been measured in buffalo calves by using a dual-isotope dilution method based on continuous infusion of (U- 14 C)D-glucose and (6- 3 H)D-glucose into the blood at a precise controlled rate for 540 min. After 5 h a plateau was obtained in the specific radioactivity of the plasma glucose from which glucose synthesis and entry rates were calculated. The average entry rates of glucose were 112 and 145 mg/min measured by 14 C and 3 H labelled glucose respectively. About 23 percent of the glucose carbon was recycled in the pool. The average recycling rate was 33 mg/min. (author)

  18. 31 CFR 337.6 - Conversions to book-entry.

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false Conversions to book-entry. 337.6... HOUSING ADMINISTRATION DEBENTURES Certificated Debentures § 337.6 Conversions to book-entry. Upon implementation of the book-entry debenture system, to be announced in advance by separate public notice, all new...

  19. 19 CFR 141.18 - Entry by nonresident corporation.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 2 2010-04-01 2010-04-01 false Entry by nonresident corporation. 141.18 Section....18 Entry by nonresident corporation. A nonresident corporation (i.e., one which is not incorporated... entry is located who is authorized to accept service of process against that corporation or, in the case...

  20. Nuclear safety policy working group recommendations on nuclear propulsion safety for the space exploration initiative

    Science.gov (United States)

    Marshall, Albert C.; Lee, James H.; Mcculloch, William H.; Sawyer, J. Charles, Jr.; Bari, Robert A.; Cullingford, Hatice S.; Hardy, Alva C.; Niederauer, George F.; Remp, Kerry; Rice, John W.

    1993-01-01

    An interagency Nuclear Safety Working Group (NSPWG) was chartered to recommend nuclear safety policy, requirements, and guidelines for the Space Exploration Initiative (SEI) nuclear propulsion program. These recommendations, which are contained in this report, should facilitate the implementation of mission planning and conceptual design studies. The NSPWG has recommended a top-level policy to provide the guiding principles for the development and implementation of the SEI nuclear propulsion safety program. In addition, the NSPWG has reviewed safety issues for nuclear propulsion and recommended top-level safety requirements and guidelines to address these issues. These recommendations should be useful for the development of the program's top-level requirements for safety functions (referred to as Safety Functional Requirements). The safety requirements and guidelines address the following topics: reactor start-up, inadvertent criticality, radiological release and exposure, disposal, entry, safeguards, risk/reliability, operational safety, ground testing, and other considerations.

  1. Entry Mode and Performance of Nordic Firms

    DEFF Research Database (Denmark)

    Wulff, Jesper

    2015-01-01

    including the proposed moderating effect, on average, yield higher post-entry performance. This study sheds light on inconsistent results found in previous research investigating the impact of international experience and has practical implications for managerial decision-making.......This study investigates whether the relationship between mode of international market entry and non-location bound international experience is weaker for firms that are large or have a high foreign to total sales ratio, labeled multinational experience. Empirical evidence based on 250 foreign...... market entries made by Norwegian, Danish and Swedish firms suggests that the association between equity mode choice and non-location bound international experience diminishes in the presence of higher levels of multinational experience. Furthermore, firms whose entry mode choice is predicted by the model...

  2. Double entry bookkeeping vs single entry bookkeeping

    OpenAIRE

    Ileana Andreica

    2016-01-01

    Abstract: A financial management eficiently begin, primarily, with an accounting record kept in the best possible conditions, this being conditioned on the adoption of a uniform forms, rational, clear and simple accounting. Throughout history, there have been known two forms of accounting: the simple and double entry. Romanian society after 1990 underwent a substantial change in social structure, the sector on which put a great emphasis being private, that of small manufacturers, ped...

  3. Nuclear Research Centre of Maamora Morocco

    International Nuclear Information System (INIS)

    Marfak, T.; Boufraqech, A.

    2010-01-01

    Morocco has a long and rich history in nuclear technology which began in the 1950s with the development of nuclear techniques in several important socio-economic fields such as medicine, agriculture and industrial applications. The development of nuclear technology evolved over various organizations, primarily within the Ministry of Education. However, with the formation of the National Centre for Nuclear Energy and Technology (CNESTEN) the development of nuclear technology in Morocco has been reinforced. Morocco is looking forward and actively pursuing alternative sources of energy and has a very strong interest in nuclear power generation and associated technologies such as nuclear desalination. Entry into these new technologies is required since there are no natural sources of energy, Morocco currently imports most of its energy needs from abroad and has a rapidly expanding energy need. In this paper, we present CNESTEN and its main facilities, missions, research programmes, human resources, training, education, national and international cooperation, etc

  4. 19 CFR 151.63 - Information on entry summary.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 2 2010-04-01 2010-04-01 false Information on entry summary. 151.63 Section 151.63 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF... Information on entry summary. Each entry summary covering wool or hair subject to duty at a rate per clean...

  5. Disturbance observer based model predictive control for accurate atmospheric entry of spacecraft

    Science.gov (United States)

    Wu, Chao; Yang, Jun; Li, Shihua; Li, Qi; Guo, Lei

    2018-05-01

    Facing the complex aerodynamic environment of Mars atmosphere, a composite atmospheric entry trajectory tracking strategy is investigated in this paper. External disturbances, initial states uncertainties and aerodynamic parameters uncertainties are the main problems. The composite strategy is designed to solve these problems and improve the accuracy of Mars atmospheric entry. This strategy includes a model predictive control for optimized trajectory tracking performance, as well as a disturbance observer based feedforward compensation for external disturbances and uncertainties attenuation. 500-run Monte Carlo simulations show that the proposed composite control scheme achieves more precise Mars atmospheric entry (3.8 km parachute deployment point distribution error) than the baseline control scheme (8.4 km) and integral control scheme (5.8 km).

  6. Heatshield for Extreme Entry Environment Technology (HEEET) Development and Maturation Status

    Science.gov (United States)

    Ellerby, D.; Boghozian, T.; Driver, D.; Chavez-Garcia, J.; Fowler, M.; Gage, P.; Gasch, M.; Gonzales, G.; Kazemba, C.; Kellermann, C.; hide

    2018-01-01

    This poster provides an overview of the requirements, design, development and testing of the 3D (Three Dimensional) Woven TPS (Thermal Protection System) being developed under NASA's Heatshield for Extreme Entry Environment Technology (HEEET) project. Under this current program, NASA is working to develop a TPS capable of surviving entry into Saturn. A primary goal of the project is to build and test an Engineering Test Unit (ETU) to establish a Technical Readiness Level (TRL) of 6 for this technology by 2017.

  7. Energy Data Base: corporate author entries

    International Nuclear Information System (INIS)

    Hendricks, P.L.

    1982-08-01

    Corporate author entries provide a means for consistent citing of the names of organizations in bibliographic records in the data bases of the DOE Technical Information Center. These entries serve as guides for users of the DOE/RECON computerized data bases who want to locate information originating in particular organizations

  8. An Examination of Market Entry Perspectives in Emerging Markets

    Directory of Open Access Journals (Sweden)

    Marvin O. Bates

    2017-11-01

    Full Text Available Purpose – The purpose of this article is to describe the marketing-oriented market entry approaches that businesses are currently using across the three levels of the world economic pyramid (i.e., WEP. These levels are the Top-tier, the Middle-tier, and the Base of the Pyramid-tier (i.e., BoP-tier. Methodology – The literature of the BoP was reviewed, and market entry approaches were itemized across the three WEP levels. Secondly, BoP strategic theorists including Prahalad identified the need for a BoP marketing focus replacing the traditional 4Ps marketing approach (i.e., Product, Price, Place and Promotion with the BoP-specific 4As marketing approach (i.e., Awareness, Affordability, Access and Availability. This 4As marketing approach is discussed. Findings – New marketing-oriented market-entry approaches are proposed for each of the three WEP levels. These approaches are based on where in the WEP the firm currently exists, and where in the WEP the firm desires to refocus market-entry activities; identified approaches include: inter-country expansion, intra-country entry, adjacent market entry, and extended market entry. Secondly, the absence of a clearly articulated marketing strategy for middle-tier markets was observed. Practical implications – This article has two specific applications. First, it summarizes the evolving market entry perspectives to provide a context for future market research in both emerging markets and the pre-emerging BoP markets. Second, the future requirement for an articulated marketing strategy for middle-tier markets is suggested. Originality – This article examined existing market entry approaches across all three levels of the WEP, inclusive of the BoP economic level. The language used to clarify market entry movements was extended, providing a specificity of description not previously found in either the existing market entry or BoP literature.

  9. Acceptions of the "death"entry in language dictionaries

    Directory of Open Access Journals (Sweden)

    Jessica Camara Siqueira

    2013-08-01

    Full Text Available Language dictionaries present the meaning of an entry in a way not restricted to linguistic information, since we also have interdisciplinary and contextual aspects that dialogue with socio-cultural issues in which the entry is inserted. Considering the potential character of the study of contextual meanings, the “death” entry was chosen to carry out a comparative analysis among language dictionaries. The choice was motivated by the recurrence of this entry in ancient and contemporary dictionaries, and the fact of its having a concept that allows various historical, social and ideological discussions. The goal is to reveal the different nuances of the “death” entry in language dictionaries, observing four main aspects: etymology, diachrony, synonymic construction and meaning of “death” in school dictionaries. Based on lexicographical studies of classical authors, we did a comparative analysis of meanings of “death” in different types of language dictionaries. We found that in each group dictionary we may find traces of ideological choices in the construction of the contextual meaning of the “death” entry.

  10. China nuclear science and technology report. Abstracts

    International Nuclear Information System (INIS)

    1994-01-01

    The bibliographies and abstracts of China Nuclear Science and Technology Reports published in 1993 (Report Numbers CNIC-00675∼CNIC-00800) are presented. The items are arranged according to INIS subject categories, which mainly are physical sciences, chemistry, materials, earth sciences, life sciences, isotopes, isotope and radiation applications, engineering and technology, and other aspects of nuclear energy. The numbers on the left corners of the entries are report numbers, and on the right corners the serial numbers. A report number index is annexed

  11. China nuclear science and technology report. Abstracts

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1994-01-01

    The bibliographies and abstracts of China Nuclear Science and Technology Reports published in 1993 (Report Numbers CNIC-00675{approx}CNIC-00800) are presented. The items are arranged according to INIS subject categories, which mainly are physical sciences, chemistry, materials, earth sciences, life sciences, isotopes, isotope and radiation applications, engineering and technology, and other aspects of nuclear energy. The numbers on the left corners of the entries are report numbers, and on the right corners the serial numbers. A report number index is annexed.

  12. Efficient nuclear export of p65-IkappaBalpha complexes requires 14-3-3 proteins.

    Science.gov (United States)

    Aguilera, Cristina; Fernández-Majada, Vanessa; Inglés-Esteve, Julia; Rodilla, Verónica; Bigas, Anna; Espinosa, Lluís

    2006-09-01

    IkappaB are responsible for maintaining p65 in the cytoplasm under non-stimulating conditions and promoting the active export of p65 from the nucleus following NFkappaB activation to terminate the signal. We now show that 14-3-3 proteins regulate the NFkappaB signaling pathway by physically interacting with p65 and IkappaBalpha proteins. We identify two functional 14-3-3 binding domains in the p65 protein involving residues 38-44 and 278-283, and map the interaction region of IkappaBalpha in residues 60-65. Mutation of these 14-3-3 binding domains in p65 or IkappaBalpha results in a predominantly nuclear distribution of both proteins. TNFalpha treatment promotes recruitment of 14-3-3 and IkappaBalpha to NFkappaB-dependent promoters and enhances the binding of 14-3-3 to p65. Disrupting 14-3-3 activity by transfection with a dominant-negative 14-3-3 leads to the accumulation of nuclear p65-IkappaBalpha complexes and the constitutive association of p65 with the chromatin. In this situation, NFkappaB-dependent genes become unresponsive to TNFalpha stimulation. Together our results indicate that 14-3-3 proteins facilitate the nuclear export of IkappaBalpha-p65 complexes and are required for the appropriate regulation of NFkappaB signaling.

  13. Modelling vocabulary development among multilingual children prior to and following the transition to school entry

    Science.gov (United States)

    MacLeod, Andrea A. N.; Castellanos-Ryan, Natalie; Parent, Sophie; Jacques, Sophie; Séguin, Jean R.

    2017-01-01

    Differences between monolingual and multilingual vocabulary development have been observed but few studies provide a longitudinal perspective on vocabulary development before and following school entry. This study compares vocabulary growth profiles of 106 multilingual children to 211 monolingual peers before and after school entry to examine whether: (1) school entry coincides with different rates of vocabulary growth compared to prior to school entry, (2) compared to monolingual peers, multilingual children show different vocabulary sizes or rates of vocabulary growth, (3) the age of onset of second-language acquisition for multilingual children is associated with vocabulary size or rate of vocabulary growth, and (4) the sociolinguistic context of the languages spoken by multilingual children is associated with vocabulary size or rate of vocabulary growth. Results showed increases in vocabulary size across time for all children, with a steeper increase prior to school entry. A significant difference between monolingual and multilingual children who speak a minority language was observed with regards to vocabulary size at school entry and vocabulary growth prior to school entry, but growth rate differences were no longer present following school entry. Taken together, results suggest that which languages children speak may matter more than being multilingual per se. PMID:29354017

  14. Modelling vocabulary development among multilingual children prior to and following the transition to school entry.

    Science.gov (United States)

    MacLeod, Andrea A N; Castellanos-Ryan, Natalie; Parent, Sophie; Jacques, Sophie; Séguin, Jean R

    2018-01-01

    Differences between monolingual and multilingual vocabulary development have been observed but few studies provide a longitudinal perspective on vocabulary development before and following school entry. This study compares vocabulary growth profiles of 106 multilingual children to 211 monolingual peers before and after school entry to examine whether: (1) school entry coincides with different rates of vocabulary growth compared to prior to school entry, (2) compared to monolingual peers, multilingual children show different vocabulary sizes or rates of vocabulary growth, (3) the age of onset of second-language acquisition for multilingual children is associated with vocabulary size or rate of vocabulary growth, and (4) the sociolinguistic context of the languages spoken by multilingual children is associated with vocabulary size or rate of vocabulary growth. Results showed increases in vocabulary size across time for all children, with a steeper increase prior to school entry. A significant difference between monolingual and multilingual children who speak a minority language was observed with regards to vocabulary size at school entry and vocabulary growth prior to school entry, but growth rate differences were no longer present following school entry. Taken together, results suggest that which languages children speak may matter more than being multilingual per se.

  15. The 3rd Sino-Japan nuclear medicine conference

    International Nuclear Information System (INIS)

    1999-01-01

    The 3rd Sino-Japan Nuclear Medicine Conference was hold on May 11-13, 1999 in Xi'an of China by Chinese Society of Nuclear Medicine, Japanese Society of Nuclear Medicine, Chinese Medicine Association and Japan-China Medicine Association. 62 articles were published in the proceeding of the conference. The contents of the articles include development and application of the radioisotopes (such as Tc-99, I-125, I-131, F-18, In-111, Tl-201, Ga-67, Sm-153, Re-188) and its radiopharmaceuticals, but application also include radiotherapy and diagnosis in the oncology and pathology by SPECT and PET

  16. The role of digital data entry in participatory environmental monitoring.

    Science.gov (United States)

    Brammer, Jeremy R; Brunet, Nicolas D; Burton, A Cole; Cuerrier, Alain; Danielsen, Finn; Dewan, Kanwaljeet; Herrmann, Thora Martina; Jackson, Micha V; Kennett, Rod; Larocque, Guillaume; Mulrennan, Monica; Pratihast, Arun Kumar; Saint-Arnaud, Marie; Scott, Colin; Humphries, Murray M

    2016-12-01

    Many argue that monitoring conducted exclusively by scientists is insufficient to address ongoing environmental challenges. One solution entails the use of mobile digital devices in participatory monitoring (PM) programs. But how digital data entry affects programs with varying levels of stakeholder participation, from nonscientists collecting field data to nonscientists administering every step of a monitoring program, remains unclear. We reviewed the successes, in terms of management interventions and sustainability, of 107 monitoring programs described in the literature (hereafter programs) and compared these with case studies from our PM experiences in Australia, Canada, Ethiopia, Ghana, Greenland, and Vietnam (hereafter cases). Our literature review showed that participatory programs were less likely to use digital devices, and 2 of our 3 more participatory cases were also slow to adopt digital data entry. Programs that were participatory and used digital devices were more likely to report management actions, which was consistent with cases in Ethiopia, Greenland, and Australia. Programs engaging volunteers were more frequently reported as ongoing, but those involving digital data entry were less often sustained when data collectors were volunteers. For the Vietnamese and Canadian cases, sustainability was undermined by a mismatch in stakeholder objectives. In the Ghanaian case, complex field protocols diminished monitoring sustainability. Innovative technologies attract interest, but the foundation of effective participatory adaptive monitoring depends more on collaboratively defined questions, objectives, conceptual models, and monitoring approaches. When this foundation is built through effective partnerships, digital data entry can enable the collection of more data of higher quality. Without this foundation, or when implemented ineffectively or unnecessarily, digital data entry can be an additional expense that distracts from core monitoring objectives

  17. Distinct hydrophobic “patches” in the N- and C-tails of beta-catenin contribute to nuclear transport

    Energy Technology Data Exchange (ETDEWEB)

    Sharma, Manisha; Jamieson, Cara; Lui, Christina [Center for Cancer Research, The Westmead Institute for Medical Research, The University of Sydney, Westmead, NSW 2145 (Australia); Henderson, Beric R., E-mail: beric.henderson@sydney.edu.au [Center for Cancer Research, The Westmead Institute for Medical Research, The University of Sydney, Westmead, NSW 2145 (Australia)

    2016-11-01

    β-catenin is a key mediator of Wnt signaling and its deregulated nuclear accumulation can drive cancer progression. While the central armadillo (Arm) repeats of β-catenin stimulate nuclear entry, the N- and C-terminal “tail” sequences are thought to regulate turnover and transactivation. We show here that the N- and C-tails are also potent transport sequences. The unstructured tails of β-catenin, when individually fused to a GFP-reporter, could enter and exit the nucleus rapidly in live cells. Proximity ligation assays and pull-down assays identified a weak interaction between the tail sequences and the FG-repeats of nucleoporins, consistent with a possible direct translocation of β-catenin through the nuclear pore complex. Extensive alanine mutagenesis of the tail sequences revealed that nuclear translocation of β-catenin was dependent on specific uniformly distributed patches of hydrophobic residues, whereas the mutagenesis of acidic amino acids had no effect. Moreover, the mutation of hydrophobic patches within the N-tail and C-tail of full length β-catenin reduced nuclear transport rate and diminished its ability to activate transcription. We propose that the tail sequences can contribute to β-catenin transport and suggest a possible similar role for hydrophobic unstructured regions in other proteins. - Highlights: • We show that the N- and C-tails of beta-catenin possess nuclear transport activity. • Nuclear transport of the N- or C-tails requires specific hydrophobic amino acids. • Mutagenesis of the N-terminus diminished nuclear entry of full-length beta-catenin. • We propose the N-tail contributes to beta-catenin nuclear entry and transactivation.

  18. Network Competition and Entry Deterrence

    OpenAIRE

    Calzada, Joan; Valletti, Tommaso

    2005-01-01

    We develop a model of logit demand that extends to a multi-firm industry the traditional duopoly framework of network competition with access charges. Firstly, we show that, when incumbents do not face the threat of entry and compete in prices, they inefficiently establish the reciprocal access charge below cost. This inefficiency disappears if incumbents compete in utilities instead of prices. Secondly, we study how incumbents change their choices under the threat of entry when they determin...

  19. Currency union entries and trade

    OpenAIRE

    Nitsch, Volker

    2005-01-01

    Recent research suggests that adopting a common currency increases bilateral trade. In this paper, I explore experiences of currency union entry in the post-war period and find no effect on trade. Previous results derived from a large panel data set (covering more than 200 countries from 1948 through 1997) appear to depend crucially on the assumption of symmetry between currency union exits and entries: While countries leaving a currency union experience significant declines in trade, currenc...

  20. The nuclear power stations of the French atomic energy programme (1960)

    International Nuclear Information System (INIS)

    Leduc, C.

    1960-01-01

    After recalling the entry of nuclear energy into energy production in France, the paper emphasizes the evolution of techniques applied in the designing of French nuclear power plants and describes the means employed for reducing costs per kWh of EDF2 and EDF3 compared with EDF1: the electric power per ton of uranium varies from 493 kW/t for EDF1 to 970 kW/t for EDF3. For this purpose the thermal power and electric power of units are changed respectively from 290 MWt for EDF1 to 1200 or 1600 MWt for EDF3 and from 28 to 250 MW. The results are obtained by an improvement in neutronic characteristics, developments in nuclear fuel technology, and simplification of the system of charging the reactor, whose means of maintenance are increased; the EDF2 heat-exchangers have been so designed as to increase the unit power of the elements, which will attain 9 MWt, as against 3 for EDF1. For EDF3 an advance project forecasts a thermodynamic layout with only one pressure stage. The paper ends with a description of the burst-slug detection systems, and an appendix gives a detailed comparative table of EDF1, EDF2 and EDF3 plant characteristics. (author) [fr

  1. 3D-grafiikka ja pelimoottorit

    OpenAIRE

    Sillanpää, Otto

    2014-01-01

    Tässä opinnäytetyössä tutkitaan miten 3D-mallit saadaan sellaiseen muotoon, että ne olisivat käytettävissä eri pelimoottoreissa. Tutkimuksen tarkoituksena on selvittää, miten luodaan 3D-malleja pelimoottoreihin, sekä miten 3D-mallinnusohjelmat ja pelimoottorit eroavat toisistaan, kun käsitellään 3D-malleja. Tässä työssä pelimoottoreina toimivat Valven Source sekä Epic Gamesin Unreal Engine 3. 3D-mallinnusohjelmista käytössä olivat Autodeskin 3ds Max 2014 ja Blender Foundationin Blender 2.7...

  2. Stanniocalcin 2 Regulates Non-capacitative Ca2+ Entry and Aggregation in Mouse Platelets

    Directory of Open Access Journals (Sweden)

    Esther López

    2018-03-01

    Full Text Available Stanniocalcin 2 (STC2 is a fish protein that controls body Ca2+ and phosphate metabolism. STC2 has also been described in mammals, and as platelet function highly depends on both extracellular and intracellular Ca2+, we have explored its expression and function in these cells. STC2−/− mice exhibit shorter tail bleeding time than WT mice. Platelets from STC2-deficient mice showed enhanced aggregation, as well as enhanced Ca2+ mobilization in response to the physiological agonist thrombin (Thr and the diacylglycerol analog, OAG, a selective activator of the non-capacitative Ca2+ entry channels. Interestingly, platelets from STC2−/− mice exhibit attenuated interaction between STIM1 and Orai1 in response to Thr, thus suggesting that STC2 is required for Thr-evoked STIM1-Orai1 interaction and the subsequent store-operated Ca2+ entry (SOCE. We have further assessed possible changes in the expression of the most relevant channels involved in non-capacitative Ca2+ entry in platelets. Then, protein expression of Orai3, TRPC3 and TRPC6 were evaluated by Western blotting, and the results revealed that while the expression of Orai3 was enhanced in the STC2-deficient mice, others like TRPC3 and TRPC6 remains almost unaltered. Summarizing, our results provide for the first time evidence for a role of STC2 in platelet physiology through the regulation of agonist-induced Ca2+ entry, which might be mediated by the regulation of Orai3 channel expression.

  3. Entry rates and recycling of glucose in buffalo calves fed on urea molasses liquid diet

    Energy Technology Data Exchange (ETDEWEB)

    Varma, A; Singh, U B; Verma, D N; Ranjhan, S K [Indian Veterinary Research Inst., Izatnagar. Div. of Animal Nutrition

    1974-12-01

    Entry rates of glucose have been measured in buffalo calves by using a dual-isotope dilution method based on continuous infusion of (U-/sup 14/C)D-glucose and (6-/sup 3/H)D-glucose into the blood at a precise controlled rate for 540 min. After 5 h a plateau was obtained in the specific radioactivity of the plasma glucose from which glucose synthesis and entry rates were calculated. The average entry rates of glucose were 112 and 145 mg/min measured by /sup 14/C and /sup 3/H labelled glucose respectively. About 23 percent of the glucose carbon was recycled in the pool. The average recycling rate was 33 mg/min.

  4. Multiple cationic amphiphiles induce a Niemann-Pick C phenotype and inhibit Ebola virus entry and infection.

    Directory of Open Access Journals (Sweden)

    Charles J Shoemaker

    Full Text Available Ebola virus (EBOV is an enveloped RNA virus that causes hemorrhagic fever in humans and non-human primates. Infection requires internalization from the cell surface and trafficking to a late endocytic compartment, where viral fusion occurs, providing a conduit for the viral genome to enter the cytoplasm and initiate replication. In a concurrent study, we identified clomiphene as a potent inhibitor of EBOV entry. Here, we screened eleven inhibitors that target the same biosynthetic pathway as clomiphene. From this screen we identified six compounds, including U18666A, that block EBOV infection (IC(50 1.6 to 8.0 µM at a late stage of entry. Intriguingly, all six are cationic amphiphiles that share additional chemical features. U18666A induces phenotypes, including cholesterol accumulation in endosomes, associated with defects in Niemann-Pick C1 protein (NPC1, a late endosomal and lysosomal protein required for EBOV entry. We tested and found that all six EBOV entry inhibitors from our screen induced cholesterol accumulation. We further showed that higher concentrations of cationic amphiphiles are required to inhibit EBOV entry into cells that overexpress NPC1 than parental cells, supporting the contention that they inhibit EBOV entry in an NPC1-dependent manner. A previously reported inhibitor, compound 3.47, inhibits EBOV entry by blocking binding of the EBOV glycoprotein to NPC1. None of the cationic amphiphiles tested had this effect. Hence, multiple cationic amphiphiles (including several FDA approved agents inhibit EBOV entry in an NPC1-dependent fashion, but by a mechanism distinct from that of compound 3.47. Our findings suggest that there are minimally two ways of perturbing NPC1-dependent pathways that can block EBOV entry, increasing the attractiveness of NPC1 as an anti-filoviral therapeutic target.

  5. SemaTyP: a knowledge graph based literature mining method for drug discovery.

    Science.gov (United States)

    Sang, Shengtian; Yang, Zhihao; Wang, Lei; Liu, Xiaoxia; Lin, Hongfei; Wang, Jian

    2018-05-30

    Drug discovery is the process through which potential new medicines are identified. High-throughput screening and computer-aided drug discovery/design are the two main drug discovery methods for now, which have successfully discovered a series of drugs. However, development of new drugs is still an extremely time-consuming and expensive process. Biomedical literature contains important clues for the identification of potential treatments. It could support experts in biomedicine on their way towards new discoveries. Here, we propose a biomedical knowledge graph-based drug discovery method called SemaTyP, which discovers candidate drugs for diseases by mining published biomedical literature. We first construct a biomedical knowledge graph with the relations extracted from biomedical abstracts, then a logistic regression model is trained by learning the semantic types of paths of known drug therapies' existing in the biomedical knowledge graph, finally the learned model is used to discover drug therapies for new diseases. The experimental results show that our method could not only effectively discover new drug therapies for new diseases, but also could provide the potential mechanism of action of the candidate drugs. In this paper we propose a novel knowledge graph based literature mining method for drug discovery. It could be a supplementary method for current drug discovery methods.

  6. The fragile peace - a nuclear review

    International Nuclear Information System (INIS)

    Anon.

    1981-01-01

    Cosmetic military superiority has reached its zenith now that the USA's superiority has been matched by that of the USSR. It is only the decision by the USA government to continue the development of the neutron warhead that could in any way maintain USA parity on the international conference bargaining tables. The USA nuclear policy is still that of deterrence. The arrival of the neutron bomb has opened a vast new horizon for use as a nuclear deterrent. On its own it seems an insignificant part of the USA nuclear arsenal. Combined as a multiple independent re-entry vehicle with any missile, it might prove to be the most fearsome weapon yet unleashed on mankind

  7. Canadian Nuclear Safety Commission's intern program

    International Nuclear Information System (INIS)

    Gilmour, P.E.

    2002-01-01

    The Intern Program was introduced at the Canadian Nuclear Safety Commission, Canada's Nuclear Regulator in response to the current competitive market for engineers and scientists and the CNSC's aging workforce. It is an entry level staff development program designed to recruit and train new engineering and science graduates to eventually regulate Canada's nuclear industry. The program provides meaningful work experience and exposes the interns to the general work activities of the Commission. It also provides them with a broad awareness of the regulatory issues in which the CNSC is involved. The intern program is a two-year program focusing on the operational areas and, more specifically, on the generalist functions of project officers. (author)

  8. China nuclear science and technology report: Abstracts, 1992

    International Nuclear Information System (INIS)

    1992-04-01

    The bibliographies and abstracts of China Nuclear Science and Technology Reports published in 1992 (Report Numbers CNIC-00555 ∼ CNIC-00674) are presented. The items are arranged according to INIS subject categories, which mainly are physics, chemistry, materials, earth sciences, life sciences, engineering and technology, and other aspects of nuclear energy. The numbers on the left corners of the entries are report numbers, and on the right corners the serial numbers. A report number index is annexed

  9. UVB-induced nuclear translocation of TC-PTP by AKT/14-3-3σ axis inhibits keratinocyte survival and proliferation.

    Science.gov (United States)

    Kim, Mihwa; Morales, Liza D; Baek, Minwoo; Slaga, Thomas J; DiGiovanni, John; Kim, Dae Joon

    2017-10-31

    Understanding protein subcellular localization is important to determining the functional role of specific proteins. T-cell protein tyrosine phosphatase (TC-PTP) contains bipartite nuclear localization signals (NLSI and NLSII) in its C-terminus. We previously have demonstrated that the nuclear form of TC-PTP (TC45) is mainly localized to the cytoplasm in keratinocytes and it is translocated to the nucleus following UVB irradiation. Here, we report that TC45 is translocated by an AKT/14-3-3σ-mediated mechanism in response to UVB exposure, resulting in increased apoptosis and decreased keratinocyte proliferation. We demonstrate that UVB irradiation increased phosphorylation of AKT and induced nuclear translocation of 14-3-3σ and TC45. However, inhibition of AKT blocked nuclear translocation of TC45 and 14-3-3σ. Site-directed mutagenesis of 14-3-3σ binding sites within TC45 showed that a substitution at Threonine 179 (TC45/T179A) effectively blocked UVB-induced nuclear translocation of ectopic TC45 due to the disruption of the direct binding between TC45 and 14-3-3σ. Overexpression of TC45/T179A in keratinocytes resulted in a decrease of UVB-induced apoptosis which corresponded to an increase in nuclear phosphorylated STAT3, and cell proliferation was higher in TC45/T179A-overexpressing keratinocytes compared to control keratinocytes following UVB irradiation. Furthermore, deletion of TC45 NLSII blocked its UVB-induced nuclear translocation, indicating that both T179 and NLSII are required. Taken together, our findings suggest that AKT and 14-3-3σ cooperatively regulate TC45 nuclear translocation in a critical step of an early protective mechanism against UVB exposure that signals the deactivation of STAT3 in order to promote keratinocyte cell death and inhibit keratinocyte proliferation.

  10. PDBsum: Structural summaries of PDB entries.

    Science.gov (United States)

    Laskowski, Roman A; Jabłońska, Jagoda; Pravda, Lukáš; Vařeková, Radka Svobodová; Thornton, Janet M

    2018-01-01

    PDBsum is a web server providing structural information on the entries in the Protein Data Bank (PDB). The analyses are primarily image-based and include protein secondary structure, protein-ligand and protein-DNA interactions, PROCHECK analyses of structural quality, and many others. The 3D structures can be viewed interactively in RasMol, PyMOL, and a JavaScript viewer called 3Dmol.js. Users can upload their own PDB files and obtain a set of password-protected PDBsum analyses for each. The server is freely accessible to all at: http://www.ebi.ac.uk/pdbsum. © 2017 The Protein Society.

  11. Orbiter Entry Aerothermodynamics Practical Engineering and Applied Research

    Science.gov (United States)

    Campbell, Charles H.

    2009-01-01

    The contents include: 1) Organization of the Orbiter Entry Aeroheating Working Group; 2) Overview of the Principal RTF Aeroheating Tools Utilized for Tile Damage Assessment; 3) Description of the Integrated Tile Damage Assessment Team Analyses Process; 4) Space Shuttle Flight Support Process; and 5) JSC Applied Aerosciences and CFD Branch Applied Research Interests.

  12. Entry of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine into the rat brain

    International Nuclear Information System (INIS)

    Riachi, N.J.; LaManna, J.C.; Harik, S.I.

    1989-01-01

    We studied blood-to-brain entry of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), 1-methyl-4-phenylpyridinium (MPP+) and butanol in anesthetized rats using the indicator-fractionation method with right atrial bolus injection. Minimal amounts of MPP+, which has low octanol/water partition coefficient, crossed the blood-brain barrier. MPTP and butanol, both of which have high octanol/water partition coefficients, were almost completely extracted by all regions of the brain on the first pass. The main difference between the MPTP and butanol tracers is that butanol rapidly left the brain with an exponential rate constant of 1.24 min-1, whereas MPTP was avidly retained by the brain with a washout rate constant of 0.10 min-1 (mean values for the four brain regions that we studied). Early retention of MPTP by the brain was not due to its rapid metabolism by monoamine oxidase because pargyline pretreatment did not affect this rate constant. However, 30 min after [ 3 H]MPTP injection, brain retention of the 3H tracer was reduced significantly by pargyline treatment, and the ratio of brain MPTP/MPP+ was increased markedly

  13. Nuclear Criticality Technology and Safety Project parameter study database

    International Nuclear Information System (INIS)

    Toffer, H.; Erickson, D.G.; Samuel, T.J.; Pearson, J.S.

    1993-03-01

    A computerized, knowledge-screened, comprehensive database of the nuclear criticality safety documentation has been assembled as part of the Nuclear Criticality Technology and Safety (NCTS) Project. The database is focused on nuclear criticality parameter studies. The database has been computerized using dBASE III Plus and can be used on a personal computer or a workstation. More than 1300 documents have been reviewed by nuclear criticality specialists over the last 5 years to produce over 800 database entries. Nuclear criticality specialists will be able to access the database and retrieve information about topical parameter studies, authors, and chronology. The database places the accumulated knowledge in the nuclear criticality area over the last 50 years at the fingertips of a criticality analyst

  14. Exploiting Herpes Simplex Virus Entry for Novel Therapeutics

    Directory of Open Access Journals (Sweden)

    Deepak Shukla

    2013-06-01

    Full Text Available Herpes Simplex virus (HSV is associated with a variety of diseases such as genital herpes and numerous ocular diseases. At the global level, high prevalence of individuals who are seropositive for HSV, combined with its inconspicuous infection, remains a cause for major concern. At the molecular level, HSV entry into a host cell involves multiple steps, primarily the interaction of viral glycoproteins with various cell surface receptors, many of which have alternate substitutes. The molecular complexity of the virus to enter a cell is also enhanced by the existence of different modes of viral entry. The availability of many entry receptors, along with a variety of entry mechanisms, has resulted in a virus that is capable of infecting virtually all cell types. While HSV uses a wide repertoire of viral and host factors in establishing infection, current therapeutics aimed against the virus are not as diversified. In this particular review, we will focus on the initial entry of the virus into the cell, while highlighting potential novel therapeutics that can control this process. Virus entry is a decisive step and effective therapeutics can translate to less virus replication, reduced cell death, and detrimental symptoms.

  15. Heat-shield for Extreme Entry Environment Technology (HEEET) Development Status

    Science.gov (United States)

    Venkatapathy, Ethiraj; Ellerby, Don; Gage, Peter

    2016-01-01

    The Heat shield for Extreme Entry Environment Technology (HEEET) Project is a NASA STMD and SMD co-funded effort. The goal is to develop and mission infuse a new ablative Thermal Protection System that can withstand extreme entry. It is targeted to support NASA's high priority missions, as defined in the latest decadal survey, to destinations such as Venus and Saturn in-situ robotic science missions. Entry into these planetary atmospheres results in extreme heating. The entry peak heat-flux and associated pressure are estimated to be between one and two orders of magnitude higher than those experienced by Mars Science Laboratory or Lunar return missions. In the recent New Frontiers community announcement NASA has indicated that it is considering providing an increase to the PI managed mission cost (PIMMC) for investigations utilizing the Heat Shield for Extreme Entry Environment Technology (HEEET) and in addition, NASA is considering limiting the risk assessment to only their accommodation on the spacecraft and the mission environment. The HEEET ablative TPS utilizes 3D weaving technology to manufacture a dual layer material architecture. The 3-D weaving allows for flat panels to be woven. The dual layer consists of a top layer designed to withstand the extreme external environment while the inner or insulating layer by design, is designed to achieve low thermal conductivity, and it keeps the heat from conducting towards the structure underneath. Both arc jet testing combined with material properties have been used to develop thermal response models that allows for comparison of performance with heritage carbon phenolic. A 50% mass efficiency is achieved by the dual layer construct compared to carbon phenolic for a broad range of missions both to Saturn and Venus. The 3-D woven flat preforms are molded to achieve the shape as they are compliant and then resin infusion with curing forms a rigid panels. These panels are then bonded on to the aeroshell structure. Gaps

  16. Market entry strategies into the BRIC countries

    DEFF Research Database (Denmark)

    Boyd, Britta; Dyhr Ulrich, Anna Marie

    2014-01-01

    Based on a sample of 177 exporting SMEs, this study investigates what market entry strategy is used by Danish family and non-family businesses. From a resource-based view, three critical internal factors (risk, flexibility and control) affecting the entry mode choice into the BRIC markets...... are analysed. The effective management of firms’ resources and capabilities is influenced by the perception of these internal factors when expanding into foreign markets. Our results confirmed that family firms build up longer lasting relationships in the host country by choosing high commitment entry modes...... when entering the BRIC markets. In contrast, family firms choose high commitment entry modes which involve high risk and low flexibility when entering the BRIC markets. Further implications discuss the suitability of export strategies to BRIC markets for managers of Danish family and non-family firms....

  17. Proceedings of NUCLEAR 2016 the 9th annual international conference on sustainable development through nuclear research and education. Part 1/3

    International Nuclear Information System (INIS)

    Paraschiv, Irina Maria

    2016-01-01

    The Proceedings of the NUCLEAR 2016 international conference on sustainable development through nuclear research and education held at INR-Pitesti on May, 18-20, contain 81 communications presented in two plenary sessions and three sections addressing the themes of Nuclear energy, Environmental protection and Sustainable development. This section (Part 1/3) are addressing the following items: Section 1.1 Nuclear safety and severe accidents (6 papers); Section 1.2 Nuclear reactors and gen. IV (7 papers); Section 1.3 Nuclear technology and materials (29 papers); These papers are presented as abstracts in 'Nuclear 2016 - Book of Abstracts', separately processed

  18. Prevent recurrence of nuclear disaster (3). Agenda on nuclear safety from earthquake engineering

    International Nuclear Information System (INIS)

    Kameda, Hiroyuki; Takada, Tsuyoshi; Ebisawa, Katsumi; Nakamura, Susumu

    2012-01-01

    Based on results of activities of committee on seismic safety of nuclear power plants (NPPs) of Japan Association for Earthquake Engineering, which started activities after Chuetsu-oki earthquake and then experienced Great East Japan Earthquake, (under close collaboration with the committee of Atomic Energy Society of Japan started activities simultaneously), and taking account of further development of concept, agenda on nuclear safety were proposed from earthquake engineering. In order to prevent recurrence of nuclear disaster, individual technical issues of earthquake engineering and comprehensive issues of integration technology, multidisciplinary collaboration and establishment of technology governance based on them were of prime importance. This article described important problems to be solved; (1) technical issues and mission of seismic safety of NPPs, (2) decision making based on risk assessment - basis of technical governance, (3) framework of risk, design and regulation - framework of required technology governance, (4) technical issues of earthquake engineering for nuclear safety, (5) role of earthquake engineering in nuclear power risk communication and (6) importance of multidisciplinary collaboration. Responsibility of engineering would be attributed to establishment of technology governance, cultivation of individual technology and integration technology, and social communications. (T. Tanaka)

  19. Entry, Descent, and Landing With Propulsive Deceleration

    Science.gov (United States)

    Palaszewski, Bryan

    2012-01-01

    The future exploration of the Solar System will require innovations in transportation and the use of entry, descent, and landing (EDL) systems at many planetary landing sites. The cost of space missions has always been prohibitive, and using the natural planetary and planet s moons atmospheres for entry, descent, and landing can reduce the cost, mass, and complexity of these missions. This paper will describe some of the EDL ideas for planetary entry and survey the overall technologies for EDL that may be attractive for future Solar System missions.

  20. Experimental study and nuclear model calculations of {sup 3}He-induced nuclear reactions on zinc

    Energy Technology Data Exchange (ETDEWEB)

    Al-Abyad, M.; Mohamed, Gehan Y. [Nuclear Research Centre, Atomic Energy Authority, Physics Department (Cyclotron Facility), Cairo (Egypt); Ditroi, F.; Takacs, S.; Tarkanyi, F. [Hungarian Academy of Sciences (ATOMKI), Institute for Nuclear Research, Debrecen (Hungary)

    2017-05-15

    Excitation functions of {sup 3}He-induced nuclear reactions on natural zinc were measured using the standard stacked-foil technique and high-resolution gamma-ray spectrometry. From their threshold energies up to 27 MeV, the cross-sections for {sup nat}Zn ({sup 3}He,xn) {sup 69}Ge, {sup nat}Zn({sup 3}He,xnp) {sup 66,67,68}Ga, and {sup nat}Zn({sup 3}He,x){sup 62,65}Zn reactions were measured. The nuclear model codes TALYS-1.6, EMPIRE-3.2 and ALICE-IPPE were used to describe the formation of these products. The present data were compared with the theoretical results and with the available experimental data. Integral yields for some important radioisotopes were determined. (orig.)

  1. Three distinct domains contribute to nuclear transport of murine Foxp3.

    Directory of Open Access Journals (Sweden)

    Wayne W Hancock

    2009-11-01

    Full Text Available Foxp3, a 47-kDa transcription factor, is necessary for the function of CD4+CD25+ regulatory T cells (Tregs, with an essential role in the control of self-reactive T cells and in preventing autoimmunity. Activation of Tregs by TCR engagement results in upregulation of Foxp3 expression, followed by its rapid nuclear transport and binding to chromatin. Here, we identify three distinct Foxp3 domains that contribute to nuclear transport. The first domain (Domain 1 comprises the C-terminal 12 amino acids. The second domain (Domain 2 is located immediately N-terminal to the forkhead domain (FHD, recently reported to be a binding site for the runt-related transcription factor 1/acute myeloid leukemia 1 (Runx1/AML1. The third domain (Domain 3 is located within the N-terminal first 51 amino acids. Unlike the known nuclear localization signals (NLSs, none of these three regions are rich in basic residues and do not bear any similarity to known monopartite or bipartite NLSs that have one or more clusters of basic amino acids. The basic arginine-lysine-lysine-arginine (RKKR sequence, located 12-aa from the C-terminal end of Foxp3 was previously reported to be a nuclear localization signal (NLS for several proteins, including for a GFP-Foxp3 hybrid. Evidence is provided here that in the full-length native Foxp3 RKKR does not function as an NLS. The data reported in this study indicates that Foxp3 achieves nuclear transport by binding to other nuclear factors and co-transporting with them to the nucleus.

  2. RITD - Re-entry: Inflatable Technology Development in Russian Collaboration

    Science.gov (United States)

    Heilimo, J.; Harri, A.-M.; Aleksashkin, S.; Koryanov, V.; Arruego, I.; Schmidt, W.; Haukka, H.; Finchenko, V.; Martynov, M.; Ostresko, B.; Ponomarenko, A.; Kazakovtsev, V.; Martin, S.; Siili, T.

    2014-04-01

    A new generation of inflatable Entry, Descent and Landing System (EDLS) for Mars has been developed. It is used in both the initial atmospheric entry and atmospheric descent before the semi-hard impact of the penetrator into Martian surface. The EDLS applicability to Earth's atmosphere is studied by the EU/RITD [1] project. Project focuses on the analysis and tests of the transonic behaviour of this compact and light weight payload entry system at the Earth re-entry.

  3. Entry Regulations, Product Differentiation and Determinants of Market Structure

    OpenAIRE

    Maican, Florin; Orth, ´Matilda

    2013-01-01

    We use a dynamic oligopoly model of entry and exit to evaluate how entry regulations affect profitability and market structure in retail. The model incorporates demand and store-level heterogeneity. Based on unique data for all retail food stores in Sweden, we find that the average entry costs for small and large stores are 10 and 18 percent lower, respectively, in markets with liberal compared with restrictive regulations. Counterfactual simulations show that lower entry costs in restrictive...

  4. Roles of outer capsid proteins as determinants of pathogenicity and host range restriction of avian rotaviruses in a suckling mouse model

    International Nuclear Information System (INIS)

    Mori, Yoshio; Borgan, Mohammed Ali; Takayama, Mutsuyo; Ito, Naoto; Sugiyama, Makoto; Minamoto, Nobuyuki

    2003-01-01

    We previously demonstrated that a pigeon rotavirus, PO-13, but not turkey strains Ty-3 and Ty-1 and a chicken strain, Ch-1, induced diarrhea in heterologous suckling mice. In this study, it was suggested that these avirulent strains, but not PO-13, were inactivated immediately in gastrointestinal tracts of suckling mice when they were orally inoculated. To determine which viral proteins contribute to the differences between the pathogenicitiy and the inactivation of PO-13 and Ty-3 in suckling mice, six PO-13 x Ty-3 reassortant strains that had the genes of the outer capsid proteins, VP4 and VP7, derived from the opposite strain were prepared and were orally inoculated to suckling mice. A single strain that had both PO-13 VP4 and VP7 with the genetic background of Ty-3 had an intermediate virulence for suckling mice. Three strains with Ty-3 VP7, regardless of the origin of VP4, rapidly disappeared from gastrointestinal tracts of suckling mice. These results indicated that the difference between the pathogenicity of PO-13 and that of Ty-3 was mainly dependent on both their VP4 and VP7. In particular, VP7 was found to be related to the inactivation of Ty-3 in gastrointestinal tracts of suckling mice

  5. Spatial and temporal variation in evacuee risk perception throughout the evacuation and return-entry process.

    Science.gov (United States)

    Siebeneck, Laura K; Cova, Thomas J

    2012-09-01

    Developing effective evacuation and return-entry plans requires understanding the spatial and temporal dimensions of risk perception experienced by evacuees throughout a disaster event. Using data gathered from the 2008 Cedar Rapids, Iowa Flood, this article explores how risk perception and location influence evacuee behavior during the evacuation and return-entry process. Three themes are discussed: (1) the spatial and temporal characteristics of risk perception throughout the evacuation and return-entry process, (2) the relationship between risk perception and household compliance with return-entry orders, and (3) the role social influences have on the timing of the return by households. The results indicate that geographic location and spatial variation of risk influenced household risk perception and compliance with return-entry plans. In addition, sociodemographic characteristics influenced the timing and characteristics of the return groups. The findings of this study advance knowledge of evacuee behavior throughout a disaster and can inform strategies used by emergency managers throughout the evacuation and return-entry process. © 2012 Society for Risk Analysis.

  6. SO(3) "Nuclear Physics" with ultracold Gases

    Science.gov (United States)

    Rico, E.; Dalmonte, M.; Zoller, P.; Banerjee, D.; Bögli, M.; Stebler, P.; Wiese, U.-J.

    2018-06-01

    An ab initio calculation of nuclear physics from Quantum Chromodynamics (QCD), the fundamental SU(3) gauge theory of the strong interaction, remains an outstanding challenge. Here, we discuss the emergence of key elements of nuclear physics using an SO(3) lattice gauge theory as a toy model for QCD. We show that this model is accessible to state-of-the-art quantum simulation experiments with ultracold atoms in an optical lattice. First, we demonstrate that our model shares characteristic many-body features with QCD, such as the spontaneous breakdown of chiral symmetry, its restoration at finite baryon density, as well as the existence of few-body bound states. Then we show that in the one-dimensional case, the dynamics in the gauge invariant sector can be encoded as a spin S = 3/2 Heisenberg model, i.e., as quantum magnetism, which has a natural realization with bosonic mixtures in optical lattices, and thus sheds light on the connection between non-Abelian gauge theories and quantum magnetism.

  7. Hepatitis C virus utilizes VLDLR as a novel entry pathway.

    Science.gov (United States)

    Ujino, Saneyuki; Nishitsuji, Hironori; Hishiki, Takayuki; Sugiyama, Kazuo; Takaku, Hiroshi; Shimotohno, Kunitada

    2016-01-05

    Various host factors are involved in the cellular entry of hepatitis C virus (HCV). In addition to the factors previously reported, we discovered that the very-low-density lipoprotein receptor (VLDLR) mediates HCV entry independent of CD81. Culturing Huh7.5 cells under hypoxic conditions significantly increased HCV entry as a result of the expression of VLDLR, which was not expressed under normoxic conditions in this cell line. Ectopic VLDLR expression conferred susceptibility to HCV entry of CD81-deficient Huh7.5 cells. Additionally, VLDLR-mediated HCV entry was not affected by the knockdown of cellular factors known to act as HCV receptors or HCV entry factors. Because VLDLR is expressed in primary human hepatocytes, our results suggest that VLDLR functions in vivo as an HCV receptor independent of canonical CD81-mediated HCV entry.

  8. Novel cyclo-peptides inhibit Ebola pseudotyped virus entry by targeting primed GP protein.

    Science.gov (United States)

    Li, Quanjie; Ma, Ling; Yi, Dongrong; Wang, Han; Wang, Jing; Zhang, Yongxin; Guo, Ying; Li, Xiaoyu; Zhou, Jinming; Shi, Yi; Gao, George F; Cen, Shan

    2018-07-01

    Ebola virus (EBOV) causes fatal hemorrhagic fever with high death rates in human. Currently, there are no available clinically-approved prophylactic or therapeutic treatments. The recently solved crystal structure of cleavage-primed EBOV glycoprotein (GPcl) in complex with the C domain of endosomal protein Niemann-Pick C1 (NPC1) provides a new target for the development of EBOV entry inhibitors. In this work, a computational approach using docking and molecular dynamic simulations is carried out for the rational design of peptide inhibitors. A novel cyclo-peptide (Pep-3.3) was identified to target at the late stage of EBOV entry and exhibit specific inhibitory activity against EBOV-GP pseudotyped viruses, with 50% inhibitory concentration (IC50) of 5.1 μM. In vitro binding assay and molecular simulations revealed that Pep-3.3 binds to GPcl with a KD value of 69.7 μM, through interacting with predicted residues in the hydrophobic binding pocket of GPcl. Mutation of predicted residues T83 caused resistance to Pep-3.3 inhibition in viral infectivity, providing preliminary support for the model of the peptide binding to GPcl. This study demonstrates the feasibility of inhibiting EBOV entry by targeting GPcl with peptides. Copyright © 2018 Elsevier B.V. All rights reserved.

  9. Analysis and Optimization of Entry Stability in Underground Longwall Mining

    Directory of Open Access Journals (Sweden)

    Yubing Gao

    2017-11-01

    Full Text Available For sustainable utilization of limited coal resources, it is important to increase the coal recovery rate and reduce mine accidents, especially those occurring in the entry (gateroad. Entry stabilities are vital for ventilation, transportation and other essential services in underground coal mining. In the present study, a finite difference model was built to investigate stress evolutions around the entry, and true triaxial tests were carried out at the laboratory to explore entry wall stabilities under different mining conditions. The modeling and experimental results indicated that a wide coal pillar was favorable for entry stabilities, but oversize pillars caused a serious waste of coal resources. As the width of the entry wall decreased, the integrated vertical stress, induced by two adjacent mining panels, coupled with each other and experienced an increase on the entry wall, which inevitably weakened the stability of the entry. Therefore, mining with coal pillars always involves a tradeoff between economy and safety. To address this problem, an innovative non-pillar mining technique by optimizing the entry surrounding structures was proposed. Numerical simulation showed that the deformation of the entry roof decreased by approximately 66% after adopting the new approach, compared with that using the conventional mining method. Field monitoring indicated that the stress condition of the entry was significantly improved and the average roof pressure decreased by appropriately 60.33% after adopting the new technique. This work provides an economical and effective approach to achieve sustainable exploitation of underground coal resources.

  10. Atmospheric Entry Experiments at IRS

    Science.gov (United States)

    Auweter-Kurtz, M.; Endlich, P.; Herdrich, G.; Kurtz, H.; Laux, T.; Löhle, S.; Nazina, N.; Pidan, S.

    2002-01-01

    3-D calculations and obtained flight data. Experience in this field and flight data are rare. But there are data bases in the USA, where public access is often difficult. The data mostly deal with reuseable launch vehi- cle technology which is a concern for the understanding of entry missions to earth and for the aim to reduce payload transportation costs in future. For X-38 about 40 international partners have been collaborating to develop, qualify and fly X-38 which is a technology demonstrator for the Crew Return Vehicle (CRV) of the International Space Station (ISS). About 1000 sensors will be operated to obtain a data base which will be combined with a aerothermodynamic data base (European contribution). Additionally, a lot of instrumentation has been contributed. PYREX-KAT38 meas- uring the temperature distribution in the X-38 nose structure was developed by IRS. The data of the PYREX sensor system contribute to several fields of interest i.e. temperature histories at 5 positons in the nose structure, information about rotational degrees of freedom of the vehicle during entry, statements on the behavior of the TPS material and heat flux distribution. The paper presents experiments that are being developed at IRS. Below summaries of such experi- ments are given: PYREX is a pyrometric entry experiment measuring rear side temperatures of ceramic TPS. It has already been flown twice. The first flight was with the German-Japanese capsule EXPRESS, the second with the capsule MIRKA. PY- REX-KAT38 was delivered to NASA. It is a fully qualified temperature measurement system and will be operated aboard the X-38 vehicle. RESPECT is a spectrometer that will be used to gain spectral data in the flow field around a space vehicle. The main goal is to obtain information about the plasma state in the post shock regime of a vehicle by measuring the spectrally resolved radiation. The obtained database will provide radiation of multiple species for a comparison with computer

  11. Non-Invasive Optical Sensor Based Approaches for Monitoring Virus Culture to Minimize BSL3 Laboratory Entry

    Directory of Open Access Journals (Sweden)

    Viswanath Ragupathy

    2015-06-01

    Full Text Available High titers of infectious viruses for vaccine and diagnostic reference panel development are made by infecting susceptible mammalian cells. Laboratory procedures are strictly performed in a Bio-Safety Level-3 (BSL3 laboratory and each entry and exit involves the use of  disposable Personnel Protective Equipment (PPE to observe cell culture conditions. Routine PPE use involves significant recurring costs. Alternative non-invasive optical sensor based approaches to remotely monitor cell culture may provide a promising and cost effective approach to monitor infectious virus cultures resulting in lower disruption and costs. We report here the monitoring of high titer cultures of Human Immunodeficiency Virus-1 (HIV-1 and Herpes Simplex Virus-2 (HSV-2 remotely with the use of optical oxygen sensors aseptically placed inside the cell culture vessel. The replacement of culture media for cell and virus propagation and virus load monitoring was effectively performed using this fluorescent sensor and resulted in half the number of visits to the BSL3 lab (five versus ten.

  12. Characterization of radon entry rates and indoor concentrations in underground structures

    International Nuclear Information System (INIS)

    Borak, T.B.; Whicker, F.W.; Fraley, L.; Gadd, M.S.; Ibrahim, S.A.; Monette, F.A.; Morris, R.; Ward, D.C.

    1992-01-01

    An experimental facility has been designed to comprehensively determine the influence of soil and meterological conditions on the transport of radon into underground structures. Two identical basements are equipped to continuously monitor pressure differentials, temperatures, soil moisture, precipitation, barometric pressure, wind speed, wind direction, natural ventiliation rates, and radon concentrations. A computerized data acquisition system accumulates and processes data at the rate of 6000 points per day. The experimental design is based on performing experiments in one structure, with the other used as a control. Indoor radon concentrations have temporal variations ranging from 150 to 1400 Bq m -3 . The corresponding entry rate of radon ranges from 300 to 10,000 Bq h -1 . When the radon entry rate is high, the indoor radon concentration decreases, whereas elevated radon concentrations seem to be associated with slow but persistent radon entry rates. This inverse relationship is partially due to compensation from enhanced natural ventilation during periods when the radon entry rate is high. Correlations between measured variables in the soil and indoor-outdoor atmospheres are used to interpret these data. This laboratory has the capability to generate essential data required for developing and testing radon transport models

  13. 2010 ANNUAL MEETING ON NUCLEAR TECHNOLOGY. Pt. 3. Section reports

    International Nuclear Information System (INIS)

    Arnold, Uwe; Baumann, Erik; Fischer, Ulrich; Bohnstedt, Angelika; Gehring, Michael; Roedig, Manfred; Willschuetz, Hans-Georg; Goers, Stefan; Schoenfelder, Christian

    2010-01-01

    Summary report on these 6 - out of 12 - Sessions of the Annual Conference on Nuclear Technology held in Berlin on May 3 to 6, 2010: - Decommissioning of Nuclear Installations (Session 7), - Fusion Technology (Session 8), - Energy Industry and Economics (Session 10), - Radiation Protection (Session 11), - New Build and Innovations (Session 12), and - Education, Expert Knowledge, Know-how-Transfer (Session 13). The other Sessions: - Reactor Physics and Methods of Calculation (Session 1), - Thermodynamics and Fluid Dynamics (Session 2), - Safety of Nuclear Installations - Methods, Analysis, Results (Session 3), - Front End and Back End of the Fuel Cycle, Radioactive Waste, Storage (Session 4), - Front End of the Fuel Cycle, Fuel Elements and Core Components (Session 5), - Operation of Nuclear Installations (Session 6) have been covered in atw issues 10 and 11 (2010). (orig.)

  14. Instrument Formatting with Computer Data Entry in Mind.

    Science.gov (United States)

    Boser, Judith A.; And Others

    Different formats for four types of research items were studied for ease of computer data entry. The types were: (1) numeric response items; (2) individual multiple choice items; (3) multiple choice items with the same response items; and (4) card column indicator placement. Each of the 13 experienced staff members of a major university's Data…

  15. Variable Entry Biased Paracentric Hemispherical Deflector: Experimental results on energy resolution for different entry positions

    Science.gov (United States)

    Dogan, Mevlut; Ulu, Melike; Gennerakis, Giannis; Zouros, Theo J. M.

    2014-04-01

    A new hemispherical deflector analyzer (HDA) which is designed for electron energy analysis in atomic collisions has been constructed and tested. Using the crossed beam technique at the electron spectrometer, test measurements were performed for electron beam (200 eV) - Helium atoms interactions. These first experimental results show that the paracentric entries give almost twice as good resolution as that for the conventional entry. Supporting simulations of the entire lens+HDA spectrometer are found in relatively good agreement with experiment.

  16. Age-dependent changes in diastolic Ca2+ and Na+ concentrations in dystrophic cardiomyopathy: Role of Ca2+ entry and IP3

    International Nuclear Information System (INIS)

    Mijares, Alfredo; Altamirano, Francisco; Kolster, Juan; Adams, José A.; López, José R.

    2014-01-01

    Highlights: • Age-dependent increase in [Ca 2+ ] d and [Na + ] d in mdx cardiomyocytes. • Gadolinium significantly reduced both [Ca 2+ ] d and [Na + ] d at all ages. • IP 3 -pathway inhibition reduced cations concentrations in dystrophic cardiomyocytes. - Abstract: Duchenne muscular dystrophy (DMD) is a lethal X-inherited disease caused by dystrophin deficiency. Besides the relatively well characterized skeletal muscle degenerative processes, DMD is also associated with a dilated cardiomyopathy that leads to progressive heart failure at the end of the second decade. The aim of the present study was to characterize the diastolic Ca 2+ concentration ([Ca 2+ ] d ) and diastolic Na + concentration ([Na + ] d ) abnormalities in cardiomyocytes isolated from 3-, 6-, 9-, and 12-month old mdx mice using ion-selective microelectrodes. In addition, the contributions of gadolinium (Gd 3+ )-sensitive Ca 2+ entry and inositol triphosphate (IP 3 ) signaling pathways in abnormal [Ca 2+ ] d and [Na + ] d were investigated. Our results showed an age-dependent increase in both [Ca 2+ ] d and [Na + ] d in dystrophic cardiomyocytes compared to those isolated from age-matched wt mice. Gd 3+ treatment significantly reduced both [Ca 2+ ] d and [Na + ] d at all ages. In addition, blockade of the IP 3 -pathway with either U-73122 or xestospongin C significantly reduced ion concentrations in dystrophic cardiomyocytes. Co-treatment with U-73122 and Gd 3+ normalized both [Ca 2+ ] d and [Na + ] d at all ages in dystrophic cardiomyocytes. These data showed that loss of dystrophin in mdx cardiomyocytes produced an age-dependent intracellular Ca 2+ and Na + overload mediated at least in part by enhanced Ca 2+ entry through Gd 3+ sensitive transient receptor potential channels (TRPC), and by IP 3 receptors

  17. Lobbying on entry

    NARCIS (Netherlands)

    Perotti, E.C.; Volpin, P.

    2004-01-01

    We develop a model of endogenous lobby formation in which wealth inequality and political accountability undermine entry and financial development. Incumbents seek a low level of effective investor protection to prevent potential entrants from raising capital. They succeed because they can promise

  18. Energy Information Data Base: corporate author entries

    International Nuclear Information System (INIS)

    1980-03-01

    One of the controls for information entered into the data bases created and maintained by the DOE Technical Information Center is the standardized name for the corporate entity or the corporate author. The purpose of Energy Information Data Base: Corporate Author Entries (TID-4585-R1) and this supplemental list of authorized or standardized corporate entries is to provide a means for the consistent citing of the names of organizations in bibliographic records. In general, an entry in Corporate Author Entries consists of the seven-digit code number assigned to the particular corporate entity, the two-letter country code, the largest element of the corporate name, the location of the corporate entity, and the smallest element of the corporate name (if provided). This supplement [DOE/TIC-4585-R1(Suppl.5)] contains additions to the base document (TID-4585-R1) and is intended to be used with that publication

  19. Science, Society, and America's Nuclear Waste: The Nuclear Waste Policy Act, Unit 3. Teacher Guide. Second Edition.

    Science.gov (United States)

    Department of Energy, Washington, DC. Office of Civilian Radioactive Waste Management, Washington, DC.

    This guide is Unit 3 of the four-part series, Science, Society, and America's Nuclear Waste, produced by the U.S. Department of Energy's Office of Civilian Radioactive Waste Management. The goal of this unit is to identify the key elements of the United States' nuclear waste dilemma and introduce the Nuclear Waste Policy Act and the role of the…

  20. Comparative study of four immortalized human brain capillary endothelial cell lines, hCMEC/D3, hBMEC, TY10, and BB19, and optimization of culture conditions, for an in vitro blood-brain barrier model for drug permeability studies.

    Science.gov (United States)

    Eigenmann, Daniela E; Xue, Gongda; Kim, Kwang S; Moses, Ashlee V; Hamburger, Matthias; Oufir, Mouhssin

    2013-11-22

    Reliable human in vitro blood-brain barrier (BBB) models suitable for high-throughput screening are urgently needed in early drug discovery and development for assessing the ability of promising bioactive compounds to overcome the BBB. To establish an improved human in vitro BBB model, we compared four currently available and well characterized immortalized human brain capillary endothelial cell lines, hCMEC/D3, hBMEC, TY10, and BB19, with respect to barrier tightness and paracellular permeability. Co-culture systems using immortalized human astrocytes (SVG-A cell line) and immortalized human pericytes (HBPCT cell line) were designed with the aim of positively influencing barrier tightness. Tight junction (TJ) formation was assessed by transendothelial electrical resistance (TEER) measurements using a conventional epithelial voltohmmeter (EVOM) and an automated CellZscope system which records TEER and cell layer capacitance (CCL) in real-time.Paracellular permeability was assessed using two fluorescent marker compounds with low BBB penetration (sodium fluorescein (Na-F) and lucifer yellow (LY)). Conditions were optimized for each endothelial cell line by screening a series of 24-well tissue culture inserts from different providers. For hBMEC cells, further optimization was carried out by varying coating material, coating procedure, cell seeding density, and growth media composition. Biochemical characterization of cell type-specific transmembrane adherens junction protein VE-cadherin and of TJ proteins ZO-1 and claudin-5 were carried out for each endothelial cell line. In addition, immunostaining for ZO-1 in hBMEC cell line was performed. The four cell lines all expressed the endothelial cell type-specific adherens junction protein VE-cadherin. The TJ protein ZO-1 was expressed in hCMEC/D3 and in hBMEC cells. ZO-1 expression could be confirmed in hBMEC cells by immunocytochemical staining. Claudin-5 expression was detected in hCMEC/D3, TY10, and at a very low level

  1. Nuclear fusion rate of the muonic T3 molecule

    International Nuclear Information System (INIS)

    Faghihi, F.; Eskandari, M. R.

    2004-01-01

    The ground state binding energy, size and effective nuclear charge of the muonic T 3 molecule are calculated using Born-Oppenheimer adiabatic approximation. The system possesses two minimum positions, one at typically muonic and the second at the atomic distances. A symmetric planar vibrational model between two minima is assumed and the approximated potential are calculated. Moreover, nuclear fusion rate calculations of the short-life molecule is carried out due to the overlap integral of the resonance nuclear compound nucleus and the molecular wave functions

  2. China nuclear science and technology report 1995. Abstracts

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-03-01

    The bibliographies and abstracts of China Nuclear Science and Technology Reports published in 1995 (Report Numbers CNIC-00921{approx}CNIC-01020) are presented. The items are arranged according to INIS subject categories, which mainly are physical sciences, chemistry, materials, earth sciences, life sciences, isotopes, isotope and radiation applications, engineering and technology, and other aspects of nuclear energy. The numbers on the left corners of the entries are report numbers, and on the right corners the serial numbers. A report number index is annexed.

  3. China nuclear science and technology report 1995. Abstracts

    International Nuclear Information System (INIS)

    1996-03-01

    The bibliographies and abstracts of China Nuclear Science and Technology Reports published in 1995 (Report Numbers CNIC-00921∼CNIC-01020) are presented. The items are arranged according to INIS subject categories, which mainly are physical sciences, chemistry, materials, earth sciences, life sciences, isotopes, isotope and radiation applications, engineering and technology, and other aspects of nuclear energy. The numbers on the left corners of the entries are report numbers, and on the right corners the serial numbers. A report number index is annexed

  4. China nuclear science and technology report (1991). Abstracts

    International Nuclear Information System (INIS)

    1992-04-01

    The bibliographies and abstracts of China Nuclear Science and Technology Reports published in 1991 (Report Numbers CNIC-00455 to CNIC-00554) are presented. The items are arranged according to INIS subject categories, which mainly are physics, chemistry, materials, earth sciences, life sciences, isotopes, isotope and radiation applications, engineering and technology, and other aspects of nuclear energy. The numbers on the left corners of the entries are report numbers, and on the right corners the serial numbers. A report number index is annexed

  5. China nuclear science and technology report abstracts 1996

    International Nuclear Information System (INIS)

    1997-10-01

    The bibliographies and abstracts of China Nuclear Science and Technology Reports published in 1996 (Report Numbers CNIC-01021∼CNIC-01130) are presented. The items are arranged according to INIS subject categories, which mainly are physical sciences, chemistry, materials, earth sciences, life sciences, isotopes, isotope and radiation applications engineering and technology, and other aspects of nuclear energy. The numbers on the left corners of the entries are report numbers, and on the right corners the serial numbers. A report number index is annexed

  6. Abstracts China nuclear science and technology report (1999)

    International Nuclear Information System (INIS)

    2001-01-01

    The bibliographies and abstracts of China Nuclear Science and Technology Reports published in 1999 (Report Numbers CNIC-01331 -CNIC-01430) are presented. The items are arranged according to INIS subject categories, which mainly are physical sciences, chemistry, materials, earth sciences, life sciences, isotopes, isotope and radiation applications, engineering and technology, and other aspects of nuclear energy. The numbers on the left corners of the entries are report numbers, and on the right corners the serial numbers. A report number index is annexed

  7. Abstracts: China Nuclear Science and Technology Report (1990)

    International Nuclear Information System (INIS)

    1991-05-01

    The bibliographies and abstracts of China Nuclear Science and Technology Reports published in 1990 (Report Numbers CNIC--00355 to CNIC-00454) are presented. The items are arranged according to INIS subjects categories, which mainly are physics, chemistry, materials, earth sciences, isotopes, isotope and radiation applications, engineering and technology, and other aspects of nuclear energy. The numbers on the left corners of the entries are report numbers, and on the right corners the serial numbers. A report number index is annexed

  8. China nuclear science and technology report. Abstracts 1994

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-02-01

    The bibliographies and abstracts of China Nuclear Science and Technology Reports published in 1994 (Report Numbers CNIC-00801{approx}CNIC-00920) are presented. The items are arranged according to INIS subject categories, which mainly are physical sciences, chemistry, materials, earth sciences, life sciences, isotopes, isotope and radiation applications, engineering and technology, and other aspects of nuclear energy. The numbers on the left corners of the entries are report numbers, and on the right corners the serial numbers. A report number index is annexed.

  9. China nuclear science and technology report abstracts 1996

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-10-01

    The bibliographies and abstracts of China Nuclear Science and Technology Reports published in 1996 (Report Numbers CNIC-01021{approx}CNIC-01130) are presented. The items are arranged according to INIS subject categories, which mainly are physical sciences, chemistry, materials, earth sciences, life sciences, isotopes, isotope and radiation applications engineering and technology, and other aspects of nuclear energy. The numbers on the left corners of the entries are report numbers, and on the right corners the serial numbers. A report number index is annexed.

  10. Abstracts China nuclear science and technology report (1999)

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-04-01

    The bibliographies and abstracts of China Nuclear Science and Technology Reports published in 1999 (Report Numbers CNIC-01331 -CNIC-01430) are presented. The items are arranged according to INIS subject categories, which mainly are physical sciences, chemistry, materials, earth sciences, life sciences, isotopes, isotope and radiation applications, engineering and technology, and other aspects of nuclear energy. The numbers on the left corners of the entries are report numbers, and on the right corners the serial numbers. A report number index is annexed.

  11. China nuclear science and technology report. Abstracts 1994

    International Nuclear Information System (INIS)

    1995-02-01

    The bibliographies and abstracts of China Nuclear Science and Technology Reports published in 1994 (Report Numbers CNIC-00801∼CNIC-00920) are presented. The items are arranged according to INIS subject categories, which mainly are physical sciences, chemistry, materials, earth sciences, life sciences, isotopes, isotope and radiation applications, engineering and technology, and other aspects of nuclear energy. The numbers on the left corners of the entries are report numbers, and on the right corners the serial numbers. A report number index is annexed

  12. Abstracts China nuclear science and technology reports (1988)

    International Nuclear Information System (INIS)

    1989-03-01

    The bibliographies and abstracts of China Nuclear Science and Technology Reports published in 1988 (Report Numbers CNIC -00115 ∼ CNIC-00254) are presented. The items are arranged according to INIS subject categories, which mainly are physics, chemistry, materials, earth sciences, life sciences, isotopes, isotope and radiation applications, engineering and technology, and other aspects of nuclear energy. The numbers on the left corners of the entries are report numbers, and on the right corners the serial numbers. A report number index is annexed

  13. Abstracts: China Nuclear Science and Technology Report (1989)

    International Nuclear Information System (INIS)

    1990-04-01

    The bibliographies and abstracts of China Nuclear Science and Technology Report published in 1989 (Report Numbers CNIC--00255∼CNIC--00354) are presented. The items are arranged according to INIS subject categories, which mainly are physics, chemistry, materials, earth sciences, life sciences, isotopes, isotope and radiation applications, engineering and technology, and other aspects of nuclear energy. The numbers on the left corners of the entries are report numbers, and on the right corners the serial numbers. A report number index is annexed

  14. The ParaShield Entry Vehicle Concept: Basic Theory and Flight Test Development

    OpenAIRE

    Akin, David

    1990-01-01

    With the emergence of microsatellite launch vehicle technology and the development of interest in space commercialization, there is a renewed need for entry vehicle technology to return mass from low earth orbit. This paper documents the ParaShield concept of the Space Systems Laboratory, which is an ultra-low ballistic coefficient (ULβ) entry vehicle. Trajectory simulations show that as the ballistic coefficient is lowered into the range of 100-150 Pa (2-3lb/ft2) the total heat load and peak...

  15. SUN1 Regulates HIV-1 Nuclear Import in a Manner Dependent on the Interaction between the Viral Capsid and Cellular Cyclophilin A.

    Science.gov (United States)

    Luo, Xinlong; Yang, Wei; Gao, Guangxia

    2018-07-01

    Human immunodeficiency virus type 1 (HIV-1) can infect nondividing cells via passing through the nuclear pore complex. The nuclear membrane-imbedded protein SUN2 was recently reported to be involved in the nuclear import of HIV-1. Whether SUN1, which shares many functional similarities with SUN2, is involved in this process remained to be explored. Here we report that overexpression of SUN1 specifically inhibited infection by HIV-1 but not that by simian immunodeficiency virus (SIV) or murine leukemia virus (MLV). Overexpression of SUN1 did not affect reverse transcription but led to reduced accumulation of the 2-long-terminal-repeat (2-LTR) circular DNA and integrated viral DNA, suggesting a block in the process of nuclear import. HIV-1 CA was mapped as a determinant for viral sensitivity to SUN1. Treatment of SUN1-expressing cells with cyclosporine (CsA) significantly reduced the sensitivity of the virus to SUN1, and an HIV-1 mutant containing CA-G89A, which does not interact with cyclophilin A (CypA), was resistant to SUN1 overexpression. Downregulation of endogenous SUN1 inhibited the nuclear entry of the wild-type virus but not that of the G89A mutant. These results indicate that SUN1 participates in the HIV-1 nuclear entry process in a manner dependent on the interaction of CA with CypA. IMPORTANCE HIV-1 infects both dividing and nondividing cells. The viral preintegration complex (PIC) can enter the nucleus through the nuclear pore complex. It has been well known that the viral protein CA plays an important role in determining the pathways by which the PIC enters the nucleus. In addition, the interaction between CA and the cellular protein CypA has been reported to be important in the selection of nuclear entry pathways, though the underlying mechanisms are not very clear. Here we show that both SUN1 overexpression and downregulation inhibited HIV-1 nuclear entry. CA played an important role in determining the sensitivity of the virus to SUN1: the regulatory

  16. 3D - Acquisition systems - test in Chooz B nuclear plant

    International Nuclear Information System (INIS)

    Brillault, B.; Thibault, G.

    1992-06-01

    EDF needs 3D-acquisition systems to get the precise geometry of critical nuclear spaces in order to prepare computer simulations of operations in these areas. The simulations must lead to an increase of the efficiency of the operation. The acquisition of the 3-D geometry can be done using 3D-acquisition systems. To answer the needs of the Construction Division, four different systems are compared by the Research Division in Chooz B nuclear plant in order to determine the right solution for each 3D-acquisition problem

  17. Mapping of nuclear import signal and importin α3 binding regions of 52K protein of bovine adenovirus-3

    International Nuclear Information System (INIS)

    Paterson, Carolyn P.; Ayalew, Lisanework E.; Tikoo, Suresh K.

    2012-01-01

    The L1 region of bovine adenovirus (BAdV)-3 encodes a non-structural protein designated 52K. Anti-52K serum detected a protein of 40 kDa, which localized to the nucleus but not to the nucleolus in BAdV-3-infected or transfected cells. Analysis of mutant 52K proteins suggested that three basic residues ( 105 RKR 107 ) of the identified domain (amino acids 102 GMPRKRVLT 110 ) are essential for nuclear localization of 52K. The nuclear import of a GST-52K fusion protein utilizes the classical importin α/β-dependent nuclear transport pathway. The 52K protein is preferentially bound to the cellular nuclear import receptor importin α3. Although deletion of amino acid 102–110 is sufficient to abrogate the nuclear localization of 52K, amino acid 90–133 are required for interaction with importin-α3 and localizing a cytoplasmic protein to the nucleus. These results suggest that 52K contains a bipartite NLS, which preferentially utilize an importin α3 nuclear import receptor-mediated pathway to transport 52K to the nucleus.

  18. 77 FR 1748 - Atomic Safety and Licensing Board; Calvert Cliffs 3 Nuclear Project, LLC, and UniStar Nuclear...

    Science.gov (United States)

    2012-01-11

    ... under represents potential contributions of wind and solar power, the combination alternative depends... Judge Ronald M. Spritzer, Atomic Safety and Licensing Board Panel, Mail Stop T-3F23, U.S. Nuclear..., Atomic Safety and Licensing Board Panel, Mail Stop T-3F23, U.S. Nuclear Regulatory Commission. Fax: (301...

  19. Nuclear power: Pt. 3

    International Nuclear Information System (INIS)

    Van Wyk, A.

    1985-01-01

    The use of nuclear power in warfare is viewed from the point of use usefullness, essentiality and demolition. The effects of a H-bomb explosion are discussed as well as the use of nuclear power in warfare, with a Christian ethical background

  20. 12 CFR 615.5451 - Book-entry and definitive securities.

    Science.gov (United States)

    2010-01-01

    ... 12 Banks and Banking 6 2010-01-01 2010-01-01 false Book-entry and definitive securities. 615.5451... AFFAIRS, LOAN POLICIES AND OPERATIONS, AND FUNDING OPERATIONS Book-Entry Procedures for Farm Credit Securities § 615.5451 Book-entry and definitive securities. Subject to subpart C of this part: (a) Farm...

Some links on this page may take you to non-federal websites. Their policies may differ from this site.