WorldWideScience

Sample records for two-semester ap biology

  1. A Two-Semester Precalculus/Calculus I Sequence: A Case Study

    Science.gov (United States)

    Axtell, Michael

    2006-01-01

    The article begins by highlighting recent trends and concerns in post-secondary Calculus and Precalculus education. The main purpose of the article is to discuss the transition to a new Precalculus/Calculus I two-semester course at Wabash College, a small liberal arts college for men. Three years of data from the earlier, traditional, sequence are…

  2. A Two-Semester Precalculus/Calculus I Sequence: A Case Study

    Science.gov (United States)

    Axtell, Michael

    2006-01-01

    The article begins by highlighting recent trends and concerns in post-secondary Calculus and Precalculus education. The main purpose of the article is to discuss the transition to a new Precalculus/Calculus I two-semester course at Wabash College, a small liberal arts college for men. Three years of data from the earlier, traditional, sequence are…

  3. Regular Biology Students Learn Like AP Students with SUN

    Science.gov (United States)

    Batiza, Ann; Luo, Wen; Zhang, Bo; Gruhl, Mary; Nelson, David; Hoelzer, Mark; Ning, Ling; Roberts, Marisa; Knopp, Jonathan; Harrington, Tom; LaFlamme, Donna; Haasch, Mary Anne; Vogt, Gina; Goodsell, David; Marcey, David

    2016-01-01

    The SUN approach to biological energy transfer education is fundamentally different from past practices that trace chemical and energy inputs and outputs. The SUN approach uses a hydrogen fuel cell to convince learners that electrons can move from one substance to another based on differential attraction. With a hydrogen fuel cell, learners can…

  4. Development of a two semester sequence of design oriented composites courses

    Energy Technology Data Exchange (ETDEWEB)

    O`Toole, B.J. [Univ. of Nevada, Las Vegas, NV (United States). Mechanical Engineering Dept.

    1994-12-31

    A two semester series of courses has been developed which is an introduction to the design, analysis, manufacturing and testing of composite materials. The courses are ideal for junior or senior level undergraduates or beginning graduate students who have no prior knowledge of composites. This sequence is well suited for a department starting to add composites courses and research activity and which only has a small number of interested faculty members. Some of the topics in this two course sequence are taken and condensed from six separate courses taught at the University of Delaware. Details about suitable textbooks, software, handouts, and resource requirements are discussed. The first course, titled ``Introduction to Composite Materials,`` includes an overview of material systems and manufacturing methods, microstructural analysis, lamina and laminate theory, design procedures, failure analysis, and the use of composites analysis software. The class is divided into small groups of students and each group selects a project early in the semester. The objective of each group is to learn enough to make competent decisions about material selection, fabrication techniques (prototype and mass production), laminate design, and failure prediction. The second course is a laboratory course which includes experimental procedures for material characterization, wet lay-up, mold making, autoclave, and pultrusion manufacturing techniques, and the design of experiments for component testing, The design groups expand their previous paper studies by fabricating and testing their designs. Student reaction has been positive and teaching these courses is enjoyable but time consuming.

  5. Development and Implementation of a Two-Semester Introductory Organic-Bioorganic Chemistry Sequence: Conclusions from the First Six Years

    Science.gov (United States)

    Goess, Brian C.

    2014-01-01

    A two-semester second-year introductory organic chemistry sequence featuring one semester of accelerated organic chemistry followed by one semester of bioorganic chemistry is described. Assessment data collected over a six-year period reveal that such a course sequence can facilitate student mastery of fundamental organic chemistry in the first…

  6. Development and Implementation of a Two-Semester Introductory Organic-Bioorganic Chemistry Sequence: Conclusions from the First Six Years

    Science.gov (United States)

    Goess, Brian C.

    2014-01-01

    A two-semester second-year introductory organic chemistry sequence featuring one semester of accelerated organic chemistry followed by one semester of bioorganic chemistry is described. Assessment data collected over a six-year period reveal that such a course sequence can facilitate student mastery of fundamental organic chemistry in the first…

  7. Deepening conceptual understanding in the high school AP biology classroom using engagement tools and techniques

    Science.gov (United States)

    Vincent, Dusti Jean

    Instructing students within a curriculum framework based on conceptual understanding requires a shift from a lecture-style, teacher-centered delivery method to one that is student-centered and inquiry-driven. A challenge with this shift is holding students accountable to preparing for course materials so that class time can be spent exploring the content in more depth through class discussions, experiential and laboratory exercises, and modeling. Three components were implemented in an AP Biology classroom of 39 students to increase engagement and accountability. These components were short readings with corresponding tutorials, formative assessments called ConcepTests, and reflective writing. Student participation in these components was measured. Conceptual understanding of biology was evaluated with a pre-test at the beginning of the term and measured again with a post-test. A Project-Based Learning (PBL) assessment was also implemented to further engage students and provide a way for students to apply their understanding to solving a real-world problem. Students demonstrated significant gains in conceptual understanding through the concept and PBL assessment. Participation in the components ranged from 73% to 86%, but it was difficult to show a positive correlation between participation and conceptual understanding.

  8. Baseline training in cognitive and psychodynamic psychotherapy during a psychologist training program. Exploring client outcomes in therapies of one or two semesters.

    Science.gov (United States)

    Dennhag, Inga; Armelius, Bengt-Åke

    2012-01-01

    This effectiveness study explored the outcomes of 187 clients seen by 187 students undergoing baseline training in psychotherapy. Clients reduced their symptoms (SCL-90) and increased their positive self-image (SASB introject) during the therapy. Multiple regression analyses showed no differences between the cognitive and the psychodynamic training approaches and no differences between one and two semesters duration of the therapies. However, 2-3% of variance in end states was accounted for by the interaction between the variables, indicating a moderating effect of duration in the two approaches. Outcomes for clients in the cognitive training approach were significantly better with two semesters than with one semester, while there was no such difference in the psychodynamic approach. Consequences for baseline training are discussed.

  9. Fluorescent biological aerosol particle concentrations and size distributions measured with an ultraviolet aerodynamic particle sizer (UV-APS) in Central Europe

    OpenAIRE

    J. A. Huffman; Treutlein, B.; U. Pöschl

    2009-01-01

    Primary Biological Aerosol Particles (PBAPs), including bacteria, spores and pollen, are essential for the spread of organisms and disease in the biosphere, and numerous studies have suggested that they may be important for atmospheric processes, including the formation of clouds and precipitation. The atmospheric abundance and size distribution of PBAPs, however, are largely unknown. At a semi-urban site in Mainz, Germany we used an Ultraviolet Aerodynamic Particle Sizer (UV-APS) to measure ...

  10. Size distributions and temporal variations of biological aerosol particles in the Amazon rainforest characterized by microscopy and real-time UV-APS fluorescence techniques during AMAZE-08

    OpenAIRE

    J. A. Huffman; Sinha, B.; R. M. Garland; A. Snee-Pollmann; Gunthe, S. S.; Artaxo, P.; S. T. Martin; Andreae, M. O.; U. Pöschl

    2012-01-01

    As a part of the AMAZE-08 campaign during the wet season in the rainforest of central Amazonia, an ultraviolet aerodynamic particle sizer (UV-APS) was operated for continuous measurements of fluorescent biological aerosol particles (FBAP). In the coarse particle size range (> 1 μm) the campaign median and quartiles of FBAP number and mass concentration were 7.3 × 104 m−3 (4.0–13.2 × 104 m−3&...

  11. Size distributions and temporal variations of biological aerosol particles in the Amazon rainforest characterized by microscopy and real-time UV-APS fluorescence techniques during AMAZE-08

    Directory of Open Access Journals (Sweden)

    J. A. Huffman

    2012-12-01

    Full Text Available As a part of the AMAZE-08 campaign during the wet season in the rainforest of central Amazonia, an ultraviolet aerodynamic particle sizer (UV-APS was operated for continuous measurements of fluorescent biological aerosol particles (FBAP. In the coarse particle size range (> 1 μm the campaign median and quartiles of FBAP number and mass concentration were 7.3 × 104 m−3 (4.0–13.2 × 104 m−3 and 0.72 μg m−3 (0.42–1.19 μg m−3, respectively, accounting for 24% (11–41% of total particle number and 47% (25–65% of total particle mass. During the five-week campaign in February–March 2008 the concentration of coarse-mode Saharan dust particles was highly variable. In contrast, FBAP concentrations remained fairly constant over the course of weeks and had a consistent daily pattern, peaking several hours before sunrise, suggesting observed FBAP was dominated by nocturnal spore emission. This conclusion was supported by the consistent FBAP number size distribution peaking at 2.3 μm, also attributed to fungal spores and mixed biological particles by scanning electron microscopy (SEM, light microscopy and biochemical staining. A second primary biological aerosol particle (PBAP mode between 0.5 and 1.0 μm was also observed by SEM, but exhibited little fluorescence and no true fungal staining. This mode may have consisted of single bacterial cells, brochosomes, various fragments of biological material, and small Chromalveolata (Chromista spores. Particles liquid-coated with mixed organic-inorganic material constituted a large fraction of observations, and these coatings contained salts likely from primary biological origin. We provide key support for the suggestion that real-time laser-induce fluorescence (LIF techniques using 355 nm excitation provide size-resolved concentrations of FBAP as a lower limit for the atmospheric abundance of biological particles in a pristine

  12. Size distributions and temporal variations of biological aerosol particles in the Amazon rainforest characterized by microscopy and real-time UV-APS fluorescence techniques during AMAZE-08

    Science.gov (United States)

    Huffman, J. A.; Sinha, B.; Garland, R. M.; Snee-Pollmann, A.; Gunthe, S. S.; Artaxo, P.; Martin, S. T.; Andreae, M. O.; Pöschl, U.

    2012-12-01

    As a part of the AMAZE-08 campaign during the wet season in the rainforest of central Amazonia, an ultraviolet aerodynamic particle sizer (UV-APS) was operated for continuous measurements of fluorescent biological aerosol particles (FBAP). In the coarse particle size range (> 1 μm) the campaign median and quartiles of FBAP number and mass concentration were 7.3 × 104 m-3 (4.0-13.2 × 104 m-3) and 0.72 μg m-3 (0.42-1.19 μg m-3), respectively, accounting for 24% (11-41%) of total particle number and 47% (25-65%) of total particle mass. During the five-week campaign in February-March 2008 the concentration of coarse-mode Saharan dust particles was highly variable. In contrast, FBAP concentrations remained fairly constant over the course of weeks and had a consistent daily pattern, peaking several hours before sunrise, suggesting observed FBAP was dominated by nocturnal spore emission. This conclusion was supported by the consistent FBAP number size distribution peaking at 2.3 μm, also attributed to fungal spores and mixed biological particles by scanning electron microscopy (SEM), light microscopy and biochemical staining. A second primary biological aerosol particle (PBAP) mode between 0.5 and 1.0 μm was also observed by SEM, but exhibited little fluorescence and no true fungal staining. This mode may have consisted of single bacterial cells, brochosomes, various fragments of biological material, and small Chromalveolata (Chromista) spores. Particles liquid-coated with mixed organic-inorganic material constituted a large fraction of observations, and these coatings contained salts likely from primary biological origin. We provide key support for the suggestion that real-time laser-induce fluorescence (LIF) techniques using 355 nm excitation provide size-resolved concentrations of FBAP as a lower limit for the atmospheric abundance of biological particles in a pristine environment. We also show some limitations of using the instrument for ambient monitoring of

  13. Fluorescent biological aerosol particle concentrations and size distributions measured with an Ultraviolet Aerodynamic Particle Sizer (UV-APS) in Central Europe

    Science.gov (United States)

    Huffman, J. A.; Treutlein, B.; Pöschl, U.

    2010-04-01

    Primary Biological Aerosol Particles (PBAPs), including bacteria, spores and pollen, are essential for the spread of organisms and disease in the biosphere, and numerous studies have suggested that they may be important for atmospheric processes, including the formation of clouds and precipitation. The atmospheric abundance and size distribution of PBAPs, however, are largely unknown. At a semi-urban site in Mainz, Germany we used an Ultraviolet Aerodynamic Particle Sizer (UV-APS) to measure Fluorescent Biological Aerosol Particles (FBAPs), which provide an estimate of viable bioaerosol particles and can be regarded as an approximate lower limit for the actual abundance of PBAPs. Fluorescence of non-biological aerosol components are likely to influence the measurement results obtained for fine particles (particles (1-20 μm). Averaged over the four-month measurement period (August-December 2006), the mean number concentration of coarse FBAPs was ~3×10-2 cm-3, corresponding to ~4% of total coarse particle number. The mean mass concentration of FBAPs was ~1μg m-3, corresponding to ~20% of total coarse particle mass. The FBAP number size distributions exhibited alternating patterns with peaks at various diameters. A pronounced peak at ~3 μm was essentially always observed and can be described by the following campaign-average lognormal fit parameters: geometric mean diameter 3.2 μm, geometric standard deviation 1.3, number concentration 1.6×10-2 cm-3. This peak is likely due to fungal spores or agglomerated bacteria, and it exhibited a pronounced diel cycle (24-h) with maximum intensity during early/mid-morning. FBAP peaks around ~1.5 μm, ~5 μm, and ~13 μm were also observed, but less pronounced and less frequent. These may be single bacterial cells, larger fungal spores, and pollen grains, respectively. The observed number concentrations and characteristic sizes of FBAPs are consistent with microscopic, biological and chemical analyses of PBAPs in aerosol

  14. Fluorescent biological aerosol particle concentrations and size distributions measured with an Ultraviolet Aerodynamic Particle Sizer (UV-APS in Central Europe

    Directory of Open Access Journals (Sweden)

    J. A. Huffman

    2010-04-01

    Full Text Available Primary Biological Aerosol Particles (PBAPs, including bacteria, spores and pollen, are essential for the spread of organisms and disease in the biosphere, and numerous studies have suggested that they may be important for atmospheric processes, including the formation of clouds and precipitation. The atmospheric abundance and size distribution of PBAPs, however, are largely unknown. At a semi-urban site in Mainz, Germany we used an Ultraviolet Aerodynamic Particle Sizer (UV-APS to measure Fluorescent Biological Aerosol Particles (FBAPs, which provide an estimate of viable bioaerosol particles and can be regarded as an approximate lower limit for the actual abundance of PBAPs. Fluorescence of non-biological aerosol components are likely to influence the measurement results obtained for fine particles (<1 μm, but not for coarse particles (1–20 μm.

    Averaged over the four-month measurement period (August–December 2006, the mean number concentration of coarse FBAPs was ~3×10−2 cm−3, corresponding to ~4% of total coarse particle number. The mean mass concentration of FBAPs was ~1μg m−3, corresponding to ~20% of total coarse particle mass. The FBAP number size distributions exhibited alternating patterns with peaks at various diameters. A pronounced peak at ~3 μm was essentially always observed and can be described by the following campaign-average lognormal fit parameters: geometric mean diameter 3.2 μm, geometric standard deviation 1.3, number concentration 1.6×10−2 cm−3. This peak is likely due to fungal spores or agglomerated bacteria, and it exhibited a pronounced diel cycle (24-h with maximum intensity during early/mid-morning. FBAP peaks around ~1.5 μm, ~5 μm, and ~13 μm were also observed, but less pronounced and less frequent. These may be single bacterial cells, larger fungal spores, and pollen grains, respectively.

    The observed number

  15. Fluorescent biological aerosol particle concentrations and size distributions measured with an ultraviolet aerodynamic particle sizer (UV-APS in Central Europe

    Directory of Open Access Journals (Sweden)

    J. A. Huffman

    2009-08-01

    Full Text Available Primary biological aerosol particles (PBAPs, including bacteria, spores and pollen, are essential for the spread of organisms and disease in the biosphere, and numerous studies have suggested that they may be important for atmospheric processes, including the formation of clouds and precipitation. The atmospheric abundance and size distribution of PBAPs, however, are largely unknown. At a semi-urban site in Mainz, Germany, we used an ultraviolet aerodynamic particle sizer (UV-APS to measure fluorescent biological aerosol particles (FBAPs, which can be regarded as viable bioaerosol particles representing a lower limit for the actual abundance of PBAPs. Fluorescence of non-biological aerosol components are likely to influence the measurement results obtained for fine particles (<1 μm, but not for coarse particles (1–20 μm.

    Averaged over the four-month measurement period (August–December 2006, the mean number concentration of coarse FBAPs was ~3×10−2 cm−3, corresponding to ~4% of total coarse particle number. The mean mass concentration of FBAPs was ~1 μg m−3, corresponding to ~20% of total coarse particle mass. The FBAP number size distributions exhibited alternating patterns with peaks at various diameters. A pronounced peak at ~3 μm was essentially always observed and can be described by the following campaign-average lognormal fit parameters: geometric mean diameter 3.2 μm, geometric standard deviation 1.3, number concentration 1.6×10−2 cm−3. This peak is likely due to fungal spores or agglomerated bacteria, and it exhibited a pronounced diel cycle with maximum intensity during early/mid-morning. FBAP peaks around ~1.5 μm, ~5 μm, and ~13 μm were also observed, but less pronounced and less frequent. These may be explained by single bacterial cells, larger fungal spores, and pollen grains, respectively.

    The observed number concentrations and

  16. AP Music Theory Applied

    Science.gov (United States)

    Spieker, Matthew H.

    2016-01-01

    Some American high schools include Advanced Placement (AP) Music Theory within their course offerings. Students who pass the AP exam can receive college credit either as a music or humanities credit. An AP class, however, offers music students more than future college credit; it ultimately improves musicianship skills and promotes deeper…

  17. AP Music Theory Applied

    Science.gov (United States)

    Spieker, Matthew H.

    2016-01-01

    Some American high schools include Advanced Placement (AP) Music Theory within their course offerings. Students who pass the AP exam can receive college credit either as a music or humanities credit. An AP class, however, offers music students more than future college credit; it ultimately improves musicianship skills and promotes deeper…

  18. Advanced Photon Source (APS)

    Data.gov (United States)

    Federal Laboratory Consortium — The Advanced Photon Source (APS) at the U.S. Department of Energy's Argonne National Laboratoryprovides this nation's (in fact, this hemisphere's) brightest storage...

  19. APS and Open Access

    Science.gov (United States)

    2011-03-01

    The movement toward Open Access continues to gain momentum. A brief review of APS efforts in this area will be presented by APS Editor in Chief, Gene Sprouse. Editors from Physical Review A, B, E, Focus, Letters, and X, Reviews of Modern Physics, and Physics will address your questions about publishing in this evolving environment.

  20. Potent quinoxaline-spaced phosphono alpha-amino acids of the AP-6 type as competitive NMDA antagonists: synthesis and biological evaluation.

    Science.gov (United States)

    Baudy, R B; Greenblatt, L P; Jirkovsky, I L; Conklin, M; Russo, R J; Bramlett, D R; Emrey, T A; Simmonds, J T; Kowal, D M; Stein, R P

    1993-02-01

    A series of alpha-amino-3-(phosphonoalkyl)-2-quinoxalinepropanoic acids was synthesized and evaluated for NMDA receptor affinity using a [3H] CPP binding assay. Functional antagonism of the NMDA receptor complex was evaluated in vitro using a stimulated [3H]TCP binding assay and in vivo by employing an NMDA-induced seizure model. Some analogues also were evaluated in the [3H]-glycine binding assay. Several compounds of the AP-6 type show potent and selective NMDA antagonistic activity both in vitro and in vivo. In particular alpha-amino-7-chloro-3-(phosphonomethyl)-2-quinoxalinepropanoic acid (1) displayed an ED50 of 1.1 mg/kg ip in the NMDA lethality model. Noteworthy is alpha-amino-6,7-dichloro-3-(phosphonomethyl)-2-quinoxalinepropanoic++ + acid (3) with a unique dual activity, displaying in the NMDA receptor binding assay an IC50 of 3.4 nM and in the glycine binding assay an IC50 of 0.61 microM.

  1. Procyanidin B2 3,3(″)-di-O-gallate, a biologically active constituent of grape seed extract, induces apoptosis in human prostate cancer cells via targeting NF-κB, Stat3, and AP1 transcription factors.

    Science.gov (United States)

    Tyagi, Alpna; Raina, Komal; Shrestha, Suraj Prakash; Miller, Bettina; Thompson, John A; Wempe, Michael F; Agarwal, Rajesh; Agarwal, Chapla

    2014-01-01

    Recently, we identified procyanidin B2 3,3(″)-di-O-gallate (B2G2) as most active constituent of grape seed extract (GSE) for efficacy against prostate cancer (PCa). Isolating large quantities of B2G2 from total GSE is labor intensive and expensive, thereby limiting both efficacy and mechanistic studies with this novel anticancer agent. Accordingly, here we synthesized gram-scale quantities of B2G2, compared it with B2G2 isolated from GSE for possible equivalent biological activity and conducted mechanistic studies. Both B2G2 preparations inhibited cell growth, decreased clonogenicity, and induced cell cycle arrest and apoptotic death, comparable to each other, in various human PCa cell lines. Mechanistic studies focusing on transcription factors involved in apoptotic and survival pathways revealed that B2G2 significantly inhibits NF-κB and activator protein1 (AP1) transcriptional activity and nuclear translocation of signal transducer and activator of transcription3 (Stat3) in PCa cell lines, irrespective of their functional androgen receptor status. B2G2 also decreased survivin expression which is regulated by NF-κB, AP1, and Stat3 and increased cleaved PARP level. In summary, we report B2G2 chemical synthesis at gram-quantity with equivalent biological efficacy against human PCa cell lines and same molecular targeting profiles at key transcription factors level. The synthetic B2G2 will stimulate more research on prostate and possibly other malignancies in preclinical models and clinical translation.

  2. APS Science 2006.

    Energy Technology Data Exchange (ETDEWEB)

    Gibson, J. M.; Fenner, R. B.; Long, G.; Borland, M.; Decker, G.

    2007-05-24

    In my five years as the Director of the Advanced Photon Source (APS), I have been fortunate to see major growth in the scientific impact from the APS. This year I am particularly enthusiastic about prospects for our longer-term future. Every scientific instrument must remain at the cutting edge to flourish. Our plans for the next generation of APS--an APS upgrade--got seriously in gear this year with strong encouragement from our users and sponsors. The most promising avenue that has emerged is the energy-recovery linac (ERL) (see article on page xx), for which we are beginning serious R&D. The ERL{at}APS would offer revolutionary performance, especially for x-ray imaging and ultrafast science, while not seriously disrupting the existing user base. I am very proud of our accelerator physics and engineering staff, who not only keep the current APS at the forefront, but were able to greatly impress our international Machine Advisory Committee with the quality of their work on the possible upgrade option (see page xx). As we prepare for long-term major upgrades, our plans to develop and optimize all the sectors at APS in the near future are advancing. Several new beamlines saw first light this year, including a dedicated powder diffraction beamline (11-BM), two instruments for inelastic x-ray scattering at sector 30, and the Center for Nanoscale Materials (CNM) Nanoprobe beamline at sector 26. Our partnership in the first x-ray free-electron laser (LCLS) to be built at Stanford contributes to revolutionary growth in ultrafast science (see page xx), and we are developing a pulse chirping scheme to get ps pulses at sector 7 of the APS within a year or so. In this report, you will find selected highlights of scientific research at the APS from calendar year 2006. The highlighted work covers diverse disciplines, from fundamental to applied science. In the article on page xx you can see the direct impact of APS research on technology. Several new products have emerged from

  3. AP statistics crash course

    CERN Document Server

    D'Alessio, Michael

    2012-01-01

    AP Statistics Crash Course - Gets You a Higher Advanced Placement Score in Less Time Crash Course is perfect for the time-crunched student, the last-minute studier, or anyone who wants a refresher on the subject. AP Statistics Crash Course gives you: Targeted, Focused Review - Study Only What You Need to Know Crash Course is based on an in-depth analysis of the AP Statistics course description outline and actual Advanced Placement test questions. It covers only the information tested on the exam, so you can make the most of your valuable study time. Our easy-to-read format covers: exploring da

  4. APS Science 2009.

    Energy Technology Data Exchange (ETDEWEB)

    Gibson, J. M; Mills, D. M.; Gerig, R.

    2010-05-01

    It is my pleasure to introduce the 2009 annual report of the Advanced Photon Source. This was a very good year for us. We operated with high reliability and availability, despite growing problems with obsolete systems, and our users produced a record output of publications. The number of user experiments increased by 14% from 2008 to more than 3600. We congratulate the recipients of the 2009 Nobel Prize in Chemistry-Venkatraman Ramakrishnan (Cambridge Institute for Medical Research), Thomas Steitz (Yale University), and Ada Yonath (Weizmann Institute) - who did a substantial amount of this work at APS beamlines. Thanks to the efforts of our users and staff, and the ongoing counsel of the APS Scientific Advisory Committee, we made major progress in advancing our planning for the upgrade of the APS (APS-U), producing a proposal that was positively reviewed. We hope to get formal approval in 2010 to begin the upgrade. With advocacy from our users and the support of our sponsor, the Office of Basic Energy Sciences in the Department of Energy (DOE) Office of Science, our operating budgets have grown to the level needed to more adequately staff our beamlines. We were also extremely fortunate to have received $7.9 M in American Recovery and Reinvestment Act ('stimulus') funding to acquire new detectors and improve several of our beamlines. The success of the new Linac Coherent Light Source at Stanford, the world's first x-ray free-electron laser, made us particularly proud since the undulators were designed and built by the APS. Among other highlights, we note that more than one-quarter of the 46 Energy Frontier Research Centers, funded competitively across the U.S. in 2009 by the DOE, included the Advanced Photon Source in their proposed work, which shows that synchrotron radiation, and the APS in particular, are central to energy research. While APS research covers everything from fundamental to applied science (reflected by the highlights in this report

  5. APS SCIENCE 2016

    Energy Technology Data Exchange (ETDEWEB)

    Fenner, Richard B. [Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

    2017-05-01

    The Advanced Photon Source (APS) occupies an 80-acre site on the Argonne national laboratory campus, about 25 miles from downtown chicago, illinois. it shares the site with the center for nanoscale materials and the Advanced Protein characterization facility. for directions to Argonne, see http://www.anl.gov/directions-and-visitor-information. The APS, a national synchrotron radiation research facility operated by Argonne for the u.S. department of energy (doe) office of Science, provides this nation’s brightest high-energy x-ray beams for science. research by APS users extends from the center of the earth to outer space, from new information on combustion engines and microcircuits to new drugs and nanotechnologies whose scale is measured in billionths of a meter. The APS helps researchers illuminate answers to the challenges of our high-tech world, from developing new forms of energy, to sustaining our nation’s technological and economic competitiveness, to pushing back against the ravages of disease. research at the APS promises to have far-reaching

  6. Akinola, AP Olokuntoye, BA

    African Journals Online (AJOL)

    ... ELASTIC THIN PLATE. Akinola, A.P. Olokuntoye, B.A.; Fadodun, 0.0. and Borokinm', A.S. ... 54 Akinola et al.: Analysis of Vibration Frequency in Transverselyisotropic Semilinear Elastic Thin Plate ... mathematically quite complex. The main ...

  7. APS Science 2007.

    Energy Technology Data Exchange (ETDEWEB)

    2008-05-30

    This report provides research highlights from the Advanced Photon Source (APS). Although these highlights represent less than 10% of the published work from the APS in 2007, they give a flavor of the diversity and impact of user research at the facility. In the strategic planning the aim is to foster the growth of existing user communities and foresee new areas of research. This coming year finds the APS engaged in putting together, along with the users, a blueprint for the next five years, and making the case for a set of prioritized investments in beamlines, the accelerator, and infrastructure, each of which will be transformational in terms of scientific impact. As this is written plans are being formulated for an important user workshop on October 20-21, 2008, to prioritize strategic plans. The fruit from past investments can be seen in this report. Examples include the creation of a dedicated beamline for x-ray photon correlation spectroscopy at Sector 8, the evolution of dedicated high-energy x-ray scattering beamlines at sectors 1 and 11, a dedicated imaging beamline at Sector 32, and new beamlines for inelastic scattering and powder diffraction. A single-pulse facility has been built in collaboration with Sector 14 (BioCARS) and Phil Anfinrud at the National Institutes of Health, which will offer exceptionally high flux for single-pulse diffraction. The nanoprobe at Sector 26, built and operated jointly by the Argonne Center for Nanoscale Materials and the X-ray Operations and Research (XOR) section of the APS X-ray Science Division, has come on line to define the state of the art in nanoscience.

  8. Factors Influencing Academic Performance of Students Enrolled in a Lower Division Cell Biology Core Course

    Science.gov (United States)

    Soto, Julio G.; Anand, Sulekha

    2009-01-01

    Students' performance in two semesters of our Cell Biology course was examined for this study. Teaching strategies, behaviors, and pre-course variables were analyzed with respect to students' performance. Pre-semester and post-semester surveys were administered to ascertain students' perceptions about class difficulty, amount of study and effort…

  9. The APS ceramic chambers

    Energy Technology Data Exchange (ETDEWEB)

    Milton, S.; Warner, D.

    1994-07-01

    Ceramics chambers are used in the Advanced Photon Source (APS) machines at the locations of the pulsed kicker and bumper magnets. The ceramic will be coated internally with a resistive paste. The resistance is chosen to allow the low frequency pulsed magnet field to penetrate but not the high frequency components of the circulating beam. Another design goal was to keep the power density experienced by the resistive coating to a minimum. These ceramics, their associated hardware, the coating process, and our recent experiences with them are described.

  10. CGM ApS Årsberetning til DANAK

    DEFF Research Database (Denmark)

    De Chiffre, Leonardo

    Denne årsberetning omfatter CGM ApS' akkrediterede virksomhed i kalenderåret 2003. Årsberetningen er udarbejdet til DANAK (Dansk Akkreditering, ErhvervsfremmeStyrelsen), som led i opfyldelsen af laboratoriets informationspligt i henhold til gældende regler.......Denne årsberetning omfatter CGM ApS' akkrediterede virksomhed i kalenderåret 2003. Årsberetningen er udarbejdet til DANAK (Dansk Akkreditering, ErhvervsfremmeStyrelsen), som led i opfyldelsen af laboratoriets informationspligt i henhold til gældende regler....

  11. AP physics B crash course

    CERN Document Server

    Howell, Rebecca

    2012-01-01

    AP Physics B Crash Course - Get a Higher Advanced Placement Score in Less Time Crash Course is perfect for the time-crunched student, the last-minute studier, or anyone who wants a refresher on the subject. Our AP Physics B Crash Course gives you: Targeted, Focused Review - Study Only What You Need to Know The Crash Course is based on an in-depth analysis of the AP Physics B course description outline and actual AP test questions. It covers only the information tested on the exam, so you can make the most of your valuable study time. Our easy-to-read format covers: mechanics, kinetic theory, t

  12. Center for Geometrisk Metrologi, CGM ApS

    DEFF Research Database (Denmark)

    De Chiffre, Leonardo

    Denne årsberetning omfatter CGM ApS' akkrediterede virksomhed i kalenderåret 2002. Årsberetningen er udarbejdet til DANAK (Dansk Akkreditering, Erhvervsfremme Styrelsen), som led i opfyldelsen af laboratoriets informationspligt i henhold til gældende regler (Teknisk Forskrift Nr. TF4 af 2000...

  13. Learning about Antiphospholipid Syndrome (APS)

    Science.gov (United States)

    ... other autoimmune disorders, including myasthenia gravis, Graves' disease, autoimmune hemolytic anemia, and Evan's syndrome. Top of page How is APS diagnosed? A diagnosis of APS is made based on both clinical and ... Autoimmune Related Diseases Association, Inc. [aarda.org] An organization ...

  14. Final report for tank 241-AP-101, grab samples 1AP-95-1, 1AP-95-2, 1AP-95-3, 1AP-95-4, 1AP-95-5, and 1AP-95-6

    Energy Technology Data Exchange (ETDEWEB)

    Esch, R.A.

    1996-03-04

    Six supernate grab samples (1AP-95-1 through 6) and one field blank (1AP-95-7) were taken from tank 241-AP-101, on Nov. 10 and 13, 1995. Analyses were performed in support of the Safety Screening and the Waste Compatibility Safety programs. All analytical results were within the action limits stated in the TSAP.

  15. Biologic

    CERN Document Server

    Kauffman, L H

    2002-01-01

    In this paper we explore the boundary between biology and the study of formal systems (logic). In the end, we arrive at a summary formalism, a chapter in "boundary mathematics" where there are not only containers but also extainers ><, entities open to interaction and distinguishing the space that they are not. The boundary algebra of containers and extainers is to biologic what boolean algebra is to classical logic. We show how this formalism encompasses significant parts of the logic of DNA replication, the Dirac formalism for quantum mechanics, formalisms for protein folding and the basic structure of the Temperley Lieb algebra at the foundations of topological invariants of knots and links.

  16. APS Editorial Q&A: APS and Open Access

    Science.gov (United States)

    2011-04-01

    The movement toward Open Access continues to gain momentum. A brief review of APS efforts in this area will be presented by APS Editor in Chief, Gene Sprouse. Editors from Physical Review C, D, Letters, and X, Physical Review Special Topics - Accelerators and Beams, Physical Review Special Topics - Physics Education Research, and Reviews of Modern Physics will address your questions about publishing in this evolving environment.

  17. Task Force on Catastrophic Antiphospholipid Syndrome (APS) and Non-criteria APS Manifestations (I): catastrophic APS, APS nephropathy and heart valve lesions.

    Science.gov (United States)

    Cervera, R; Tektonidou, M G; Espinosa, G; Cabral, A R; González, E B; Erkan, D; Vadya, S; Adrogué, H E; Solomon, M; Zandman-Goddard, G; Shoenfeld, Y

    2011-02-01

    The objectives of the 'Task Force on Catastrophic Antiphospholipid Syndrome (APS) and Non-criteria APS Manifestations' were to assess the clinical utility of the international consensus statement on classification criteria and treatment guidelines for the catastrophic APS, to identify and grade the studies that analyse the relationship between the antiphospholipid antibodies and the non-criteria APS manifestations and to present the current evidence regarding the accuracy of these non-criteria APS manifestations for the detection of patients with APS. This article summarizes the studies analysed on the catastrophic APS, APS nephropathy and heart valve lesions, and presents the recommendations elaborated by the Task Force after this analysis.

  18. [Apheresis in antiphospholipid syndrome (APS)].

    Science.gov (United States)

    De Silvestro, Giustina; Tison, Tiziana; Marson, Piero

    2012-01-01

    Antiphospholipid syndrome (APS) is a rare clinical disorder characterized by thromboembolic manifestations and/or obstetric complications. Along with the clinical symptoms and signs, serum antiphospholipid antibodies have to be detected. APS can be primary, i.e., without any concomitant disorders, or secondary to other autoimmune diseases, particularly systemic lupus erythematosus. Criteria for the diagnosis of APS have been clearly established. Hyperacute APS (or catastrophic antiphospholipid syndrome), often with a poor prognosis, must meet four criteria: involvement of three or more organs, rapid evolution of clinical manifestations, microangiopathic occlusion of small blood vessels at biopsy, and presence of antiphospholipid antibodies. The rationale for apheresis treatment is the removal of pathogenetic antibodies involved in the development of tissue damage. Our experience includes 23 patients, in particular 15 women treated for 19 pregnancies. According to the National Guidelines Program, the effectiveness of apheresis in catastrophic syndrome has a level of evidence of V/VI, with a strength of recommendation A; in highrisk pregnancy it has a level of evidence of V with a strength of recommendation B. It will be necessary to better define the prognosis of various categories of pregnant patients with APS, as well as useful laboratory parameters to monitor its clinical course and anticipate any complications of pregnancy.

  19. APS Education and Diversity Efforts

    Science.gov (United States)

    Prestridge, Katherine; Hodapp, Theodore

    2015-11-01

    American Physical Society (APS) has a wide range of education and diversity programs and activities, including programs that improve physics education, increase diversity, provide outreach to the public, and impact public policy. We present the latest programs spearheaded by the Committee on the Status of Women in Physics (CSWP), with highlights from other diversity and education efforts. The CSWP is working to increase the fraction of women in physics, understand and implement solutions for gender-specific issues, enhance professional development opportunities for women in physics, and remedy issues that impact gender inequality in physics. The Conferences for Undergraduate Women in Physics, Professional Skills Development Workshops, and our new Professional Skills program for students and postdocs are all working towards meeting these goals. The CSWP also has site visit and conversation visit programs, where department chairs request that the APS assess the climate for women in their departments or facilitate climate discussions. APS also has two significant programs to increase participation by underrepresented minorities (URM). The newest program, the APS National Mentoring Community, is working to provide mentoring to URM undergraduates, and the APS Bridge Program is an established effort that is dramatically increasing the number of URM PhDs in physics.

  20. AP Geography, Environmental Science Thrive

    Science.gov (United States)

    Robelen, Erik W.

    2012-01-01

    Geography may not be particularly known as a hot topic among today's students--even some advocates suggest it suffers from an image problem--but by at least one measure, the subject is starting to come into its own. Across more than 30 topics covered in the Advanced Placement (AP) program, participation in geography is rising faster than any…

  1. AP Geography, Environmental Science Thrive

    Science.gov (United States)

    Robelen, Erik W.

    2012-01-01

    Geography may not be particularly known as a hot topic among today's students--even some advocates suggest it suffers from an image problem--but by at least one measure, the subject is starting to come into its own. Across more than 30 topics covered in the Advanced Placement (AP) program, participation in geography is rising faster than any…

  2. Science Café Course: An Innovative Means of Improving Communication Skills of Undergraduate Biology Majors

    OpenAIRE

    Anna Goldina; Weeks, Ophelia I.

    2013-01-01

    To help bridge the increasing gap between scientists and the public, we developed an innovative two-semester course called Science Café. In this course, undergraduate biology majors learn to develop communication skills to be better able to explain science concepts and current developments in science to non-scientists. Students develop and host outreach events on various topics relevant to the community, thereby increasing interactions between budding scientists and the public. Such a Science...

  3. An AP Calculus Classroom Amusement Park

    Science.gov (United States)

    Ferguson, Sarah

    2016-01-01

    Throughout the school year, AP Calculus teachers strive to teach course content comprehensively and swiftly in an effort to finish all required material before the AP Calculus exam. As early May approaches and the AP Calculus test looms, students and teachers nervously complete lessons, assignments, and assessments to ensure student preparation.…

  4. An AP Calculus Classroom Amusement Park

    Science.gov (United States)

    Ferguson, Sarah

    2016-01-01

    Throughout the school year, AP Calculus teachers strive to teach course content comprehensively and swiftly in an effort to finish all required material before the AP Calculus exam. As early May approaches and the AP Calculus test looms, students and teachers nervously complete lessons, assignments, and assessments to ensure student preparation.…

  5. Fellow's Apéro

    CERN Multimedia

    Staff Association

    2017-01-01

    Let's get together, meet each other, exchange experiences and ideas, and share useful information on CERN and the Staff Association. Join us for Fellow's Apéro, organised by the Staff Association on Tuesday 21 February at 16.30 in Restaurant 1. There will be drinks and snacks for everybody! We look forward to seeing you there! Please confirm your participation on Doodle http://doodle.com/poll/skvm7ucm2z78i6bt or alternatively on Facebook https://www.facebook.com/events/1862757017340069/. Your delegates in the Staff Association, Barbora & Jiri

  6. Testing of Solar Heated Domestic Hot Water System for Solahart Scandinavia ApS

    DEFF Research Database (Denmark)

    Andersen, Elsa

    1997-01-01

    The solar heating system marketed by Solahart Scandinavia ApS was tested in the Institutes test facility for SDHWsystems. The test results are described in the report.......The solar heating system marketed by Solahart Scandinavia ApS was tested in the Institutes test facility for SDHWsystems. The test results are described in the report....

  7. Toward university modeling instruction--biology: adapting curricular frameworks from physics to biology.

    Science.gov (United States)

    Manthey, Seth; Brewe, Eric

    2013-06-01

    University Modeling Instruction (UMI) is an approach to curriculum and pedagogy that focuses instruction on engaging students in building, validating, and deploying scientific models. Modeling Instruction has been successfully implemented in both high school and university physics courses. Studies within the physics education research (PER) community have identified UMI's positive impacts on learning gains, equity, attitudinal shifts, and self-efficacy. While the success of this pedagogical approach has been recognized within the physics community, the use of models and modeling practices is still being developed for biology. Drawing from the existing research on UMI in physics, we describe the theoretical foundations of UMI and how UMI can be adapted to include an emphasis on models and modeling for undergraduate introductory biology courses. In particular, we discuss our ongoing work to develop a framework for the first semester of a two-semester introductory biology course sequence by identifying the essential basic models for an introductory biology course sequence.

  8. APS high heat load monochromator

    Energy Technology Data Exchange (ETDEWEB)

    Lee, W.K.; Mills, D.

    1993-02-01

    This document contains the design specifications of the APS high heat load (HHL) monochromator and associated accessories as of February 1993. It should be noted that work is continuing on many parts of the monochromator including the mechanical design, crystal cooling designs, etc. Where appropriate, we have tried to add supporting documentation, references to published papers, and calculations from which we based our decisions. The underlying philosophy behind performance specifications of this monochromator was to fabricate a device that would be useful to as many APS users as possible, that is, the design should be as generic as possible. In other words, we believe that this design will be capable of operating on both bending magnet and ID beamlines (with the appropriate changes to the cooling and crystals) with both flat and inclined crystal geometries and with a variety of coolants. It was strongly felt that this monochromator should have good energy scanning capabilities over the classical energy range of about 4 to 20 keywith Si (111) crystals. For this reason, a design incorporating one rotation stage to drive both the first and second crystals was considered most promising. Separate rotary stages for the first and second crystals can sometimes provide more flexibility in their capacities to carry heavy loads (for heavily cooled first crystals or sagittal benders of second crystals), but their tuning capabilities were considered inferior to the single axis approach.

  9. Diadenosine polyphosphates Ap3A and Ap4A, but not Ap5A or Ap6A, induce proliferation of vascular smooth muscle cells.

    Science.gov (United States)

    Bobbert, Peter; Schlüter, Hartmut; Schultheiss, Heinz Peter; Reusch, Hans Peter

    2008-05-15

    Depending on the number of phosphate groups, diadenosine polyphosphates (ApnA, Ap3A, Ap4A, Ap5A and Ap6A) differ in properties such as proliferation, apoptosis, vasoconstriction and vasodilatation of vascular smooth muscle cells (VSMCs). Possible signaling pathways leading to effects such as proliferation are still unknown. This study examined the proliferative effects of diadenosine polyphosphates on VSMCs and their intracellular pathways. Proliferation of VSMCs was measured by the cell count and [(3)H] thymidine incorporation. Phosphorylation of the MAP kinases ERK1/2 was determined by Western blotting. Single-cell [Ca(2+)](i) measurements were done to determine the influence of [Ca(2+)](i) on intracellular signaling. Stress fiber formation was assessed by fluorescence microscopy to detect an influence of G alpha(12). Ap3A and Ap4A, but not Ap5A or Ap6A, were shown to increase proliferation of VSMCs by activating P2Y receptors, which leads to stimulation of the Ras-Raf-MEK-ERK1/2 cascade. Ap3A- and Ap4A-induced activation of the MAP kinases ERK1/2 was dependent on a signaling pathway that included the EGF receptor, PKC, PLCbeta and the increase of [Ca(2+)](i). In conclusion, Ap3A and Ap4A, but not Ap5A or Ap6A, induce proliferation of VSMCs by a signaling pathway that begins with activation of P2Y receptors and leads to stimulation of the MAP kinases ERK1/2.

  10. On AP-property of Function Spaces

    Institute of Scientific and Technical Information of China (English)

    SHEN Rong-xin

    2012-01-01

    In this paper,it is discussed the AP-property of function spaces.We prove that for any compact network α for a space X which is closed under finite unions,(1) if Cα(X) is an AP-space and X is paracompact,then X is a Hurewicz space; (2) if Cα (X) is an AP-space which has countable tightness,then Cα(X) is discretely generated.

  11. AP calculus AB & BC crash course

    CERN Document Server

    Rosebush, J

    2012-01-01

    AP Calculus AB & BC Crash Course - Gets You a Higher Advanced Placement Score in Less Time Crash Course is perfect for the time-crunched student, the last-minute studier, or anyone who wants a refresher on the subject. AP Calculus AB & BC Crash Course gives you: Targeted, Focused Review - Study Only What You Need to Know Crash Course is based on an in-depth analysis of the AP Calculus AB & BC course description outline and actual AP test questions. It covers only the information tested on the exams, so you can make the most of your valuable study time. Written by experienced math teachers, our

  12. Trypanosoma cruzi strains: behavior after passage into authoctonous or foreign species of triatomine (biological and biochemical patterns Cepas do Trypanosoma cruzi: comportamento após passagem em triatomíneos autóctones ou não autóctones (padrões Biológicos e Bioquímicos

    Directory of Open Access Journals (Sweden)

    Juracy B Magalhães

    1996-02-01

    Full Text Available The behavior of T. cruzi strains from S. Felipe - BA (19 SF, 21 SF and 22 SF classified as Type II Zymodeme 2, was investigated after passage through the authoctonous (P. megistus and foreign vectors (T. infestans and R. prolixus. For each strain Swiss mice were infected: I - with blood forms (control; II - with metacyclic forms (MF from P. megistus; III - with MF from T. infestans; IV - with MF from R. prolixus. Inocula: MF from the three species of triatomine, 60 to 120 days after feeding in infected mice, adjusted to 10 4. Biological behavior in mice (parasitemia, morphology, mortality, virulence and pathogenicity after passage through triatomine was compared with data from the same strain in control mice. Isoenzymic electrophoresis (ASAT, ALAT, PGM, GPI were also performed after culture into Warren medium. The three strains maintained the isoenzyme profiles (zymodeme 2, in the control groups and after passages through different species of triatomine. Biological characterization disclosed Type II strains patterns for all groups. An increased virulence was observed with the 22 SF strain isolated from P. megistus and T. infestans and higher levels of parasitemia and predominance of slender forms in mice inoculated with the 19 SF and 21 SF from these same species. Results indicate that the passage through the two species T. infestans and P. megistus had a positive influence on the virulence of the regional strains of S. Felipe, regardless of being autocthonous (P. megistus or foreign to the area (T. infestans.Foi estudada a influência da passagem de cepas do T. cruzi de São Felipe - BA (19 SF, 21 SF e 22 SF, Tipo II, Zimodema 2, no vetor autóctone (P. megistus e em vetores não autóctones (T. infestans e R. prolixus. Para cada cepa, camundongos suíços de 10 a 12 g foram inoculados com I - formas sanguícolas (controles; II - metacíclicos do P. megistus; III - metacíclicos de T. infestans; IV - metacíclicos do R. prolixus. O in

  13. Functions and Application of the AP2/ERF Transcription Factor Family in Crop Improvement

    Institute of Scientific and Technical Information of China (English)

    Zhao-Shi Xu; Ming Chen; Lian-Cheng Li; You-Zhi Ma

    2011-01-01

    Plants have acquired sophisticated stress response systems to adapt to changing environments. It is important to understand plants' stress response mechanisms in the effort to improve crop productivity under stressful conditions. The AP2/ERF transcription factors are known to regulate diverse processes of plant development and stress responses.In this study, the molecular characteristics and biological functions of AP2/ERFs in a variety of plant species were analyzed. AP2/ERFs,especially those in DREB and ERF subfamilies, are ideal candidates for crop improvement because their overexpression enhances tolerances to drought, salt, freezing, as well as resistances to multiple diseases in the transgenic plants. The comprehensive analysis of physiological functions is useful in elucidating the biological roles of AP2/ERF family genes in gene interaction, pathway regulation, and defense response under stress environments, which should provide new opportunities for the crop tolerance engineering.

  14. Consumo de antimicrobianos en APS

    Directory of Open Access Journals (Sweden)

    María Cristina Lara Bastanzuri

    2003-08-01

    Full Text Available Con el propósito de describir el patrón de uso de los medicamentos para el tratamiento con antimicrobianos en APS en Cuba en el período de 1989 al 2000, se diseñó un estudio descriptivo, observacional y retrospectivo clasificado dentro de los estudios de utilización de medicamentos como de consumo. Para ello se calcularon las dosis diarias definidas cada día de determinado fármaco de los grupos clasificados según ATC como tetraciclinas, anfenicoles, penicilinas de amplio espectro, cefalosporinas, sulfonamidas y trimetoprim, macrólidos, estreptomicinas y quinolonas. Además, se tomaron las cifras de pacientes inscriptos a penicilina benzatínica y se comparó con la población expuesta obtenida a partir de las DHD. Las penicilinas son las de mayor consumo con tendencia al aumento, igual que los aminoglucósidos, mientras que la tetraciclina presenta cifras mayores de DHD. La tendencia del cloranfenicol es a disminuir. La población expuesta está muy por debajo de los pacientes inscriptos en penicilina benzatínica y las líneas de tendencia no son similares. Excepto la docixiclina, el resto de los antimicrobianos recomendados en la Guía Terapéutica para APS se encuentran en el Listado Básico de Medicamentos del país para el nivel primario de atención médica.With the objective of describing the pattern of drug use in antimicrobial-based treatment in the primary health care in Cuba from 1989 to 2000, we designed a retrospective descriptive and observational study, classified into the study of drug use as consumption. To this end, the daily doses of certain drugs from the ATC-classified groups were calculated, which included tetracycline, amphenicols, broad spectrum penicilins, cephalosporins, sulfonamides, trimethoprim, macrolides, streptomycin and quinolones. Also, the number of patients registered as benzathine peniciline consumers was taken and compared to the exposed population data obtained from the DHD. Penicillins are the

  15. ELECTROCHEMICAL CORROSION TESTING OF TANKS 241-AN-102 & 241-AP-107 & 241-AP-108 IN SUPPORT OF ULTRASONIC TESTING

    Energy Technology Data Exchange (ETDEWEB)

    WYRWAS RB; DUNCAN JB

    2008-11-20

    This report presents the results of the corrosion rates that were measured using electrochemical methods for tanks 241-AN-102 (AN-102), 241-AP-107 (AP 107), and 241-AP-108 (AP-108) performed under test plant RPP-PLAN-38215. The steel used as materials of construction for AN and AP tank farms was A537 Class 1. Test coupons of A537 Class 1 carbon steel were used for corrosion testing in the AN-107, AP-107, and AP-108 tank waste. Supernate will be tested from AN-102, AP-107, and Ap-108. Saltcake testing was performed on AP-108 only.

  16. AP English language & composition crash course

    CERN Document Server

    Hogue, Dawn

    2012-01-01

    AP English Language & Composition Crash Course - Gets You a Higher Advanced Placement Score in Less Time Crash Course is perfect for the time-crunched student, the last-minute studier, or anyone who wants a refresher on the subject. AP English Language & Composition Crash Course gives you: Targeted, Focused Review - Study Only What You Need to Know Crash Course is based on an in-depth analysis of the AP English Language & Composition course description outline and actual Advanced Placement test questions. It covers only the information tested on the exam, so you can make the most of your valua

  17. Long-term stability of the APS storage ring

    Energy Technology Data Exchange (ETDEWEB)

    Friedsam, H.; Penicka, M.; Error, J.

    1999-10-26

    The Advanced Photon Source (APS), a third-generation synchrotrons light source, was commissioned in 1995 at Argonne ''National Laboratory and has been fully-operational for beamline users since 1997. The APS storage ring can accommodate up to 68 user beamlines; about 70% of the available beamlines are currently in use by various collaborative access teams (CATS). The 7-GeV synchrotrons light source produces light in the soft to hard x-ray range that is used for research in such areas as x-ray instrumentation; material, chemical and atomic sciences; biology; and geo/soil/ environmental sciences. For the successful operation of an x-ray light source such as the Advanced Photon Source, the long-term stability of the concrete floor supporting the beam components and user beamlines is crucial. Settlements impact the orbit and location of the x-ray source points as well as the position of the x-ray beamlines. This paper compares the results of two successive resurveys of the APS accelerator components performed in 1995 and 1998.

  18. Pentoxifylline Treatment in Acute Pancreatitis (AP)

    Science.gov (United States)

    2016-09-14

    Acute Pancreatitis (AP); Gallstone Pancreatitis; Alcoholic Pancreatitis; Post-ERCP/Post-procedural Pancreatitis; Trauma Acute Pancreatitis; Hypertriglyceridemia Acute Pancreatitis; Idiopathic (Unknown) Acute Pancreatitis; Medication Induced Acute Pancreatitis; Cancer Acute Pancreatitis; Miscellaneous (i.e. Acute on Chronic Pancreatitis)

  19. AP1000{sup TM} plant modularization

    Energy Technology Data Exchange (ETDEWEB)

    Cantarero L, C.; Demetri, K. J. [Westinghouse Electric Co., 1000 Westinghouse Drive, Cranberry Township, PA 16066 (United States); Quintero C, F. P., E-mail: cantarc@westinghouse.com [Westinghouse Electric Spain, Padilla 17, 28006 Madrid (Spain)

    2016-09-15

    The AP1000{sup TM} plant is an 1100 M We pressurized water reactor (PWR) with passive safety features and extensive plant simplifications that enhance construction, operation, maintenance and safety. Modules are used extensively in the design of the AP1000 plant nuclear island. The AP1000 plant uses modern, modular-construction techniques for plant construction. The design incorporates vendor-designed skids and equipment packages, as well as large, multi-ton structural modules and special equipment modules. Modularization allows traditionally sequential construction tasks to be completed simultaneously. Factory-built modules can be installed at the site in a planned construction schedule. The modularized AP1000 plant allows many more construction activities to proceed in parallel. This reduces plant construction calendar time, thus lowering the costs of plant financing. Furthermore, performing less work onsite significantly reduces the amount of skilled field-craft labor, which costs more than shop labor. In addition to labor cost savings, doing more welding and fabrication in a factory environment raises the quality of work, allowing more scheduling flexibility and reducing the amount of specialized tools required onsite. The site layout for the AP1000 plant has been established to support modular construction and efficient operations during construction. The plant layout is compact, using less space than previous conventional plant layouts. This paper provides and overview of the AP1000 plant modules with an emphasis on structural modules. Currently the Westinghouse AP1000 plant has four units under construction in China and four units under construction in the United States. All have shown successful fabrication and installation of various AP1000 plant modules. (Author)

  20. Peculiar Traits of Coarse AP (Briefing Charts)

    Science.gov (United States)

    2014-12-01

    Self Combustion 3 Thom L. Boggs Parallel ridges and valley surfaces seen at 1000 to 1500 psi Isolated AP vertical needle pockets at 1500 and 1800 psi...Negative exponents, bouncing flamlets between 2000 to 4000 psi Extremely irregular AP surface regression Hightower and Price 16 Air Force Research...supported by vertical gas jetlets from active centers? Did Boggs burn a few samples at 1000 to 2000 psi with orthorhombic main axis normal to

  1. Tank 241-AP-106, Grab samples, 6AP-98-1, 6AP-98-2 and 6AP-98-3 Analytical results for the final report

    Energy Technology Data Exchange (ETDEWEB)

    FULLER, R.K.

    1999-02-23

    This document is the final report for tank 241-AP-106 grab samples. Three grab samples 6AP-98-1, 6AP-98-2 and 6AP-98-3 were taken from riser 1 of tank 241-AP-106 on May 28, 1998 and received by the 222-S Laboratory on May 28, 1998. Analyses were performed in accordance with the ''Compatability Grab Sampling and Analysis Plan'' (TSAP) (Sasaki, 1998) and the ''Data Quality Objectives for Tank Farms Waste Compatability Program (DQO). The analytical results are presented in the data summary report. No notification limits were exceeded. The request for sample analysis received for AP-106 indicated that the samples were polychlorinated biphenyl (PCB) suspects. The results of this analysis indicated that no PCBs were present at the Toxic Substance Control Act (TSCA) regulated limit of 50 ppm. The results and raw data for the PCB analysis are included in this document.

  2. Kepler observations of rapidly oscillating Ap, δ Scuti and γ Doradus pulsations in Ap stars

    DEFF Research Database (Denmark)

    Balona, Luis A.; Cunha, Margarida S.; Kurtz, Donald W.

    2011-01-01

    Observations of the A5p star KIC 8677585 obtained during the Kepler 10-d commissioning run with 1-min time resolution show that it is a rapidly oscillating Ap (roAp) star with several frequencies with periods near 10 min. In addition, a low frequency at 3.142 d−1 is also clearly present....... Multiperiodic γ Doradus (γ Dor) and δ Scuti (δ Sct) pulsations, never before seen in any Ap star, are present in Kepler observations of at least three other Ap stars. Since γ Dor pulsations are seen in Ap stars, it is likely that the low frequency in KIC 8677585 is also a γ Dor pulsation. The simultaneous...... presence of both γ Dor and roAp pulsations and the unexpected detection of δ Sct and γ Dor pulsations in Ap stars present new opportunities and challenges for the interpretation of these stars. Since it is easy to confuse Am and Ap stars at classification dispersions, the nature of these Ap stars...

  3. Low concentrations of copper in drinking water increase AP-1 binding in the brain.

    Science.gov (United States)

    Lung, Shyang; Li, Huihui; Bondy, Stephen C; Campbell, Arezoo

    2015-12-01

    Copper (Cu) in trace amounts is essential for biological organisms. However, dysregulation of the redox-active metal has been implicated in different neurological disorders such as Wilson's, Menkes', Alzheimer's, and Parkinson's diseases. Since many households use Cu tubing in the plumbing system, and corrosion causes the metal to leach into the drinking water, there may be adverse effects on the central nervous system connected with low-level chronic exposure. The present study demonstrates that treatment with a biologically relevant concentration of Cu for 3 months significantly increases activation of the redox-modulated transcription factor AP-1 in mouse brains. This was independent of an upstream kinase indicated in AP-1 activation. Another redox-active transcription factor, NF-κB, was not significantly modified by the Cu exposure. These results indicate that the effect of Cu on AP-1 is unique and may involve direct modulation of DNA binding.

  4. Multiplicity of rapidly oscillating Ap stars

    CERN Document Server

    Schoeller, M; Hubrig, S; Kurtz, D W

    2012-01-01

    Rapidly oscillating Ap (roAp) stars have rarely been found in binary or higher order multiple systems. This might have implications for their origin. We intend to study the multiplicity of this type of chemically peculiar stars, looking for visual companions in the range of angular separation between 0.05" and 8". We carried out a survey of 28 roAp stars using diffraction-limited near-infrared imaging with NAOS-CONICA at the VLT. Additionally, we observed three non-oscillating magnetic Ap stars. We detected a total of six companion candidates with low chance projection probabilities. Four of these are new detections, the other two are confirmations. An additional 39 companion candidates are very likely chance projections. We also found one binary system among the non-oscillating magnetic Ap stars. The detected companion candidates have apparent K magnitudes between 6.8 and 19.5 and angular separations ranging from 0.23" to 8.9", corresponding to linear projected separations of 30-2400AU. While our study confi...

  5. Down-regulation of AP-4 inhibits proliferation, induces cell cycle arrest and promotes apoptosis in human gastric cancer cells.

    Directory of Open Access Journals (Sweden)

    Xinghua Liu

    Full Text Available BACKGROUND: AP-4 belongs to the basic helix-loop-helix leucine-zipper subgroup; it controls target gene expression, regulates growth, development and cell apoptosis and has been implicated in tumorigenesis. Our previous studies indicated that AP-4 was frequently overexpressed in gastric cancers and may be associated with the poor prognosis. The purpose of this study is to examine whether silencing of AP-4 can alter biological characteristics of gastric cancer cells. METHODS: Two specific siRNAs targeting AP-4 were designed, synthesized, and transfected into gastric cancer cell lines and human normal mucosa cells. AP-4 expression was measured with real-time quantitative PCR and Western blot. Cell proliferation and chemo-sensitivity were detected by CCK-8 assay. Cell cycle assay and apoptosis assay were performed by flow cytometer, and relative expression of cell cycle regulators were detected by real-time quantitative PCR and Western blot, expression of the factors involved in the apoptosis pathway were examined in mRNA and protein level. RESULTS: The expression of AP-4 was silenced by the siRNAs transfection and the effects of AP-4 knockdown lasted 24 to 96 hrs. The siRNA-mediated silencing of AP-4 suppressed the cellular proliferation, induced apoptosis and sensitized cancer cells to anticancer drugs. In addition, the expression level of p21, p53 and Caspase-9 were increased when AP-4 was knockdown, but the expression of cyclin D1, Bcl-2 and Bcl-x(L was inhibited. It didn't induce cell cycle arrest when AP-4 was knockdown in p53 defect gastric cancer cell line Kato-III. CONCLUSIONS: These results illustrated that gene silencing of AP-4 can efficiently inhibited cell proliferation, triggered apoptosis and sensitized cancer cells to anticancer drugs in vitro, suggesting that AP-4 siRNAs mediated silencing has a potential value in the treatment of human gastric cancer.

  6. Center for Geometrisk Metrologi CGM ApS, Årsberetning 2001 til DANAK

    DEFF Research Database (Denmark)

    De Chiffre, Leonardo

    Denne årsberetning omfatter CGM ApS' akkrediterede virksomhed i kalenderåret 2001. Årsberetningen er udarbejdet til DANAK (Dansk Akkreditering, Erhvervsfremme Styrelsen), som led i opfyldelsen af laboratoriets informationspligt i henhold til gældende regler (Teknisk Forskrift Nr. TF4 af 2000...

  7. AP1000, a nuclear central of advanced design; AP1000, una central nuclear de diseno avanzado

    Energy Technology Data Exchange (ETDEWEB)

    Hernandez M, N.; Viais J, J. [ININ, 52045 Ocoyoacac, Estado de Mexico (Mexico)]. e-mail: nhm@nuclear.inin.mx

    2005-07-01

    The AP1000 is a design of a nuclear reactor of pressurized water (PWR) of 1000 M We with characteristic of safety in a passive way; besides presenting simplifications in the systems of the plant, the construction, the maintenance and the safety, the AP1000 is a design that uses technology endorsed by those but of 30 years of operational experience of the PWR reactors. The program AP1000 of Westinghouse is focused to the implementation of the plant to provide improvements in the economy of the same one and it is a design that is derived directly of the AP600 designs. On September 13, 2004 the US-NRC (for their initials in United States- Nuclear Regulatory Commission) approved the final design of the AP1000, now Westinghouse and the US-NRC are working on the whole in a complete program for the certification. (Author)

  8. An analysis of AP600 design features

    Energy Technology Data Exchange (ETDEWEB)

    Park, Jong Kyoon; Jang, Moon Heui; Hwang, Yung Dong [Korea Atomic Energy Research Institute, Taejon (Korea, Republic of)] [and others

    1994-01-01

    In the aspect of engineering, passive safety system concept has improved the safety degree of nuclear power plant. Therefore, the objective of this study is to check on the possibility of the capacity upgrade of nuclear power plant in the case of adopting the passive safety system concept of AP 600. The characteristics of AP 600 are the advanced functions in ECCS, heat removal of containment building and residual heat removal under the passive safety system concept. The result of this study will become the basic data of capacity upgrade of nuclear power plant and will be widely used in second year project. (Author).

  9. Formalistic approaches to A&P

    Institute of Scientific and Technical Information of China (English)

    赵倩

    2014-01-01

    A&P is a famous short story. Its simple words, the unique point of view and skillful writing technique are expressed successfully. Besides, the first-person narrator is also a good point. From Sammy’s eyes, readers can learn the character’s characteristics. Another point is the usage of symbol, the name of the store, A&P, it shows that people’s life is rich, but their spirit is barren. This article tries to analyze it by formalistic approaches.

  10. Chlamydia trachomatis CT771 (nudH) is an asymmetric Ap4A hydrolase

    Science.gov (United States)

    Barta, Michael L.; Lovell, Scott; Sinclair, Amy N.; Battaile, Kevin P.; Hefty, P. Scott

    2014-01-01

    Asymmetric diadenosine 5′,5′″-P1,P4-tetraphosphate (Ap4A) hydrolases are members of the Nudix superfamily that asymmetrically cleave the metabolite Ap4A into ATP and AMP while facilitating homeostasis. The obligate intracellular mammalian pathogen Chlamydia trachomatis possesses a single Nudix family protein, CT771. As pathogens that rely on a host for replication and dissemination typically have one or zero Nudix family proteins, this suggests that CT771 could be critical for chlamydial biology and pathogenesis. We identified orthologs to CT771 within environmental Chlamydiales that share active site residues suggesting a common function. Crystal structures of both apo- and ligand-bound CT771 were determined to 2.6 Å and 1.9 Å resolution, respectively. The structure of CT771 shows a αβα-sandwich motif with many conserved elements lining the putative Nudix active site. Numerous aspects of the ligand-bound CT771 structure mirror those observed in the ligand-bound structure of the Ap4A hydrolase from Caenorhabditis elegans. These structures represent only the second Ap4A hydrolase enzyme member determined from eubacteria and suggest that mammalian and bacterial Ap4A hydrolases might be more similar than previously thought. The aforementioned structural similarities, in tandem with molecular docking, guided the enzymatic characterization of CT771. Together, these studies provide the molecular details for substrate binding and specificity, supporting the analysis that CT771 is an Ap4A hydrolase (nudH). PMID:24354275

  11. EjAP2-1, an AP2/ERF gene, is a novel regulator of fruit lignification induced by chilling injury, via interaction with EjMYB transcription factors.

    Science.gov (United States)

    Zeng, Jiao-Ke; Li, Xian; Xu, Qian; Chen, Jian-Ye; Yin, Xue-Ren; Ferguson, Ian B; Chen, Kun-Song

    2015-12-01

    Lignin biosynthesis is regulated by many transcription factors, such as those of the MYB and NAC families. However, the roles of AP2/ERF transcription factors in lignin biosynthesis have been rarely investigated. Eighteen EjAP2/ERF genes were isolated from loquat fruit (Eriobotrya japonica), which undergoes postharvest lignification during low temperature storage. Among these, expression of EjAP2-1, a transcriptional repressor, was negatively correlated with fruit lignification. The dual-luciferase assay indicated that EjAP2-1 could trans-repress activities of promoters of lignin biosynthesis genes from both Arabidopsis and loquat. However, EjAP2-1 did not interact with the target promoters (Ej4CL1). Yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays indicated protein-protein interactions between EjAP2-1 and lignin biosynthesis-related EjMYB1 and EjMYB2. Furthermore, repression effects on the Ej4CL1 promoter were observed with the combination of EjAP2-1 and EjMYB1 or EjMYB2, while EjAP2-1 with the EAR motif mutated (mEjAP2-1) lost such repression, although mEjAP2-1 still interacted with EjMYB protein. Based on these results, it is proposed that EjAP2-1 is an indirect transcriptional repressor on lignin biosynthesis, and the repression effects were manifested by EAR motifs and were conducted via protein-protein interaction with EjMYBs. © 2015 Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd.

  12. Two Successful Approaches to Teaching AP Government

    Science.gov (United States)

    Ladd, Brian; Stepp, Heidi

    2013-01-01

    Amador Valley High School, in Pleasanton, California, uses two unique approaches to teaching Advanced Placement Government and Politics. AP Government consists of six units: Constitutional Underpinnings; Political Behavior and Political Beliefs; Mass Media, Interest Groups, and Political Parties; Institutions of Government; Civil Liberties and…

  13. Barron's AP English literature and composition

    CERN Document Server

    Ehrenhaft EdD, George

    2016-01-01

    Includes five full-length practice AP exams with all questions answered and explained. Also features additional reviews on poetry, fiction, and drama, definitions of 175 literary and rhetorical terms, and more. Can be purchased alone or with an optional CD-ROM with two additional practice tests.

  14. Structuring the AP Art History Course

    Science.gov (United States)

    Herscher, Walter R.

    2013-01-01

    While AP (Advanced Placement) Art History may be taught within the art department in many schools, social studies teachers are equally capable of teaching the course well. They have the historical background to discuss the reasons for changes in art styles. A teacher's preparation is similar to teaching a course stressing political history,…

  15. 2000 Johnston Site 1A-P

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Underwater Site 1A-P was established at Johnston Atoll by Dr. James Maragos, U.S. Fish & Wildlife Service, on June 29, 2000. With a start point (meter 0) at...

  16. 2000 Johnston Site 2A-P

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Underwater Site 2A-P was established at Johnston Atoll by Dr. James Maragos, U.S. Fish & Wildlife Service, on June 30, 2000. With a start point (meter 0) at...

  17. 2000 Johnston Site 3A-P

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Underwater Site 3A-P was established at Johnston Atoll by Dr. James Maragos, U.S. Fish & Wildlife Service, on July 3, 2000. With a start point (meter 0) at...

  18. 2006 Johnston Site 1A-P

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Underwater Site 1A-P was established at Johnston Atoll by Dr. James Maragos, U.S. Fish & Wildlife Service, on June 29, 2000. With a start point (meter 0) at...

  19. Quassinoid inhibition of AP-1 function does not correlate with cytotoxicity or protein synthesis inhibition.

    Science.gov (United States)

    Beutler, John A; Kang, Moon-Il; Robert, Francis; Clement, Jason A; Pelletier, Jerry; Colburn, Nancy H; McKee, Tawnya C; Goncharova, Ekaterina; McMahon, James B; Henrich, Curtis J

    2009-03-27

    Several quassinoids were identified in a high-throughput screening assay as inhibitors of the transcription factor AP-1. Further biological characterization revealed that while their effect was not specific to AP-1, protein synthesis inhibition and cell growth assays were inconsistent with a mechanism of simple protein synthesis inhibition. Numerous plant extracts from the plant family Simaroubaceae were also identified in the same screen; bioassay-guided fractionation of one extract (Ailanthus triphylla) yielded two known quassinoids, ailanthinone (3) and glaucarubinone (4), which were also identified in the pure compound screening procedure.

  20. AE8/AP8 Implementations in AE9/AP9, IRBEM, and SPENVIS

    Science.gov (United States)

    2014-02-18

    period applies to orbit generation only; AE8/AP8 utilizes geomagnetic field models from other epochs as specified in the table below.) SHIELDOSE2 model...Cain, J. C., S. J. Hendricks, R. A. Langel, and W. V. Hudson (1967), A proposed model for the international geomagnetic reference field, 1965, J...trapped particle fluxes with the NASA models AP-8 and AE-8, Radiat. Meas., 26, pp. 947-952. International Association of Geomagnetism and Aeronomy

  1. Comparative analysis between radiographic views for knee osteoarthrosis (bipedal AP versus monopedal AP

    Directory of Open Access Journals (Sweden)

    Rodrigo Pires e Albuquerque

    2013-08-01

    Full Text Available OBJECTIVE: A comparative analysis by applying the criteria of the original classification Ahlbäck in the anteroposterior (AP bipedal knee in extension and anteroposterior (AP monopodal knee in symptomatic knee arthrosis. With this analysis we intend to observe the agreement, any advantage or difference between the incidence and degree of joint involvement between the orthopedic surgeons and radiologists with the referring physician. METHODS: From January 2012 to March 2012, was a prospective study of 60 symptomatic arthrosis knees (60 patients, clinically selected group of outpatient knee and radiographic proposals submitted to the search. Of the 60 patients, 39 were female and 21 male, mean age 64 years (ranging from 50 to 84 years. Of the 60 knees studied, 37 corresponded to the right side and 23 on the left side. Statistical analysis was performed by Kappa statistics, which evaluates the interobserver agreement for qualitative data. RESULTS: According to the scale of Ahlbäck, there was a significant agreement (p < 0.0001 intra-observer in the classification of knee osteoarthritis among the five evaluators. There was a significant agreement (p < 0.0001 with inter-observer referring physician in the incidence of AP monopodal and AP bipedal for the four raters. CONCLUSION: The study found no difference between the incidence in the AP monopodal versus AP bipedal in osteoarthritis of the knee.

  2. CLEARING MAGNET DESIGN FOR APS-U

    Energy Technology Data Exchange (ETDEWEB)

    Abliz, M.; Grimmer, J.; Jaski, Y.; Westferro, F.; Ramanathan, M.

    2017-06-25

    The Advanced Photon Source is in the process of developing an upgrade (APS-U) of the storage ring. The upgrade will be converting the current double bend achromat (DBA) lattice to a multi-bend achromat (MBA) lattice. In addition, the storage ring will be operated at 6 GeV and 200 mA with regular swap-out injection to keep the stored beam current constant [1]. The swap-out injection will take place with beamline shutters open. For radiation safety to ensure that no electrons can exit the storage ring, a passive method of protecting the beamline and containing the electrons inside the storage ring is proposed. A clearing magnet will be located in all beamline front ends inside the storage ring tunnel. This article will discuss the features and design of the clearing magnet scheme for APS-U.

  3. AP stars with resolved Zeeman split lines

    Science.gov (United States)

    Mathys, G.

    1990-06-01

    High-resolution, high SNR observations of a sample of sharp-lined A stars and of Ap stars showing resolved Zeeman split lines are presented. The Fe II lines 6147.7 A and 6149.2 A unexpectedly appear to be asymmetric in all stars where they are resolved. The blue component of the 6149.2 line, which is a Zeeman doublet, is deeper and narrower than its red component. For line 6147.7, whose Zeeman pattern does not differ much from a quadruplet, the red components are deeper than the blue ones. It is shown that a partial Paschen-Back effect can account for these properties. The potential implications of this finding for studies of magnetic Ap stars are discussed.

  4. Final report for tank 241-AP-108, grab samples 8AP-96-1, 8AP-96-2 and 8AP-96-FB

    Energy Technology Data Exchange (ETDEWEB)

    Esch, R.A.

    1996-04-19

    This document is the final report deliverable for the tank 241-AP-108 grab samples. The samples were subsampled and analyzed in accordance with the TSAP. Included in this report are the results for the Waste Compatibility analyses, with the exception of DSC and thermogravimetric analysis (TGA) results which were presented in the 45 Day report (Part 2 of this document). The raw data for all analyses, with the exception of DSC and TGA, are also included in this report.

  5. An Updated AP2 Beamline TURTLE Model

    Energy Technology Data Exchange (ETDEWEB)

    Gormley, M.; O' Day, S.

    1991-08-23

    This note describes a TURTLE model of the AP2 beamline. This model was created by D. Johnson and improved by J. Hangst. The authors of this note have made additional improvements which reflect recent element and magnet setting changes. The magnet characteristics measurements and survey data compiled to update the model will be presented. A printout of the actual TURTLE deck may be found in appendix A.

  6. Cultural Diversity in AP Art History

    Science.gov (United States)

    Bolte, Frances R.

    2006-01-01

    Teaching AP Art History is like running on a treadmill that is moving faster than a teacher can run. Many teachers are out of breath before the end of the term and wonder how in the world they can cover every chapter. Because time is short and art from pre-history through to the present, including the non-European traditions, must be covered, this…

  7. Using DCAT-AP for research data

    OpenAIRE

    PEREGO ANDREA; FRIIS-CHRISTENSEN Anders; VACCARI LORENZINO; TSINARAKI CHRYSI

    2016-01-01

    This paper outlines a set cross-domain requirements for the documentation of scientific data, identified during the development of the corporate data catalogue of the European Commission's Joint Research Centre (JRC). In particular, we illustrate how we have extended the DCAT application profile for European data portals (DCAT-AP) to accomodate requirements for scientific datasets, and we discuss a number of issues still to be addressed.

  8. Task Force on Catastrophic Antiphospholipid Syndrome (APS) and Non-criteria APS Manifestations (II): thrombocytopenia and skin manifestations.

    Science.gov (United States)

    Cervera, R; Tektonidou, M G; Espinosa, G; Cabral, A R; González, E B; Erkan, D; Vadya, S; Adrogué, H E; Solomon, M; Zandman-Goddard, G; Shoenfeld, Y

    2011-02-01

    The objectives of the 'Task Force on Catastrophic Antiphospholipid Syndrome (APS) and Non-criteria APS Manifestations' were to assess the clinical utility of the international consensus statement on classification criteria and treatment guidelines for the catastrophic APS, to identify and grade the studies that analyze the relationship between the antiphospholipid antibodies and the non-criteria APS manifestations, and to present the current evidence regarding the accuracy of these non-criteria APS manifestations for the detection of patients with APS. This article summarizes the studies analyzed on thrombocytopenia and skin manifestations, and presents the recommendations elaborated by the Task Force after this analysis.

  9. AIRE variations in Addison's disease and autoimmune polyendocrine syndromes (APS)

    DEFF Research Database (Denmark)

    Bøe Wolff, A S; Oftedal, B; Johansson, S

    2008-01-01

    Autoimmune Addison's disease (AAD) is often associated with other components in autoimmune polyendocrine syndromes (APS). Whereas APS I is caused by mutations in the AIRE gene, the susceptibility genes for AAD and APS II are unclear. In the present study, we investigated whether polymorphisms...... or copy number variations in the AIRE gene were associated with AAD and APS II. First, nine SNPs in the AIRE gene were analyzed in 311 patients with AAD and APS II and 521 healthy controls, identifying no associated risk. Second, in a subgroup of 25 of these patients, AIRE sequencing revealed three novel...... polymorphisms. Finally, the AIRE copy number was determined by duplex quantitative PCR in 14 patients with APS I, 161 patients with AAD and APS II and in 39 healthy subjects. In two Scandinavian APS I patients previously reported to be homozygous for common AIRE mutations, we identified large deletions...

  10. Características biológicas do solo indicadoras de qualidade após dois anos de aplicação de biossólido industrial e cultivo de milho Biological characteristics indicators of soil quality after two years of application of an industrial biosolid and corn cultivation

    Directory of Open Access Journals (Sweden)

    Isabel Cristina de Barros Trannin

    2007-10-01

    Full Text Available A utilização agrícola de biossólidos tem sido muito incentivada, mas, como esses resíduos apresentam composição química variada, o valor agronômico e os efeitos sobre características indicadoras de qualidade do solo precisam ser avaliados caso a caso, a fim de estabelecer normas de segurança para uso desses materiais. Neste trabalho foram avaliadas características biológicas, após a aplicação, por dois anos consecutivos, de doses crescentes (0, 6, 12, 18 e 24 t ha-1 base seca de um biossólido gerado por uma indústria de fibras e resinas PET e da adubação mineral completa no cultivo de milho, em um Cambissolo distrófico, comparados aos de uma área adjacente, sob Brachiaria sp. e sem cultivo nos últimos 10 anos, usada como referência. Os valores de C e N da biomassa microbiana, a respiração basal e as atividades das enzimas urease e beta-glicosidase e da hidrólise do diacetato de fluoresceína (FDA aumentaram, enquanto a atividade da fosfatase ácida diminuiu com a elevação das doses de biossólido, porém estas não tiveram efeito sobre o quociente metabólico (qCO2. A diminuição da atividade da fosfatase se deveu ao aumento da disponibilidade de P no solo, não caracterizando efeito adverso da aplicação do biossólido. Com aplicação de 12 t ha-1 de biossólido (recomendação agronômica, a respiração e a hidrólise da FDA foram maiores e a atividade da fosfatase foi menor que a obtida no solo com adubação mineral, mas as demais características avaliadas não diferiram entre estes tratamentos. A colonização micorrízica de Brachiaria sp. não diferiu entre plantas de crescimento espontâneo nas parcelas anteriormente cultivadas com milho e aquelas da área adjacente. Apesar do menor número de esporos, verificou-se enriquecimento de espécies de fungos micorrízicos arbusculares (FMAs nas parcelas cultivadas. O carbono orgânico (Corg e a biomassa microbiana apresentaram alta correlação com os demais

  11. Analysis list: CASP8AP2 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available CASP8AP2 Digestive tract + hg19 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/target/CAS...P8AP2.1.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/target/CASP8AP2.5.tsv http://dbarchive.bi...osciencedbc.jp/kyushu-u/hg19/target/CASP8AP2.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/colo/CAS

  12. Teaching Materials and Strategies for the AP Music Theory Exam

    Science.gov (United States)

    Lively, Michael T.

    2017-01-01

    Each year, many students take the Advanced Placement (AP) Music Theory Exam, and the majority of these students enroll in specialized AP music theory classes as part of the preparation process. For the teachers of these AP music theory classes, a number of challenges are presented by the difficulty and complexity of the exam subject material as…

  13. First molluscan transcription factor activator protein-1 (Ap-1) member from disk abalone and its expression profiling against immune challenge and tissue injury.

    Science.gov (United States)

    De Zoysa, Mahanama; Nikapitiya, Chamilani; Lee, Youngdeuk; Lee, Sukkyoung; Oh, Chulhong; Whang, Ilson; Yeo, Sang-Yeop; Choi, Cheol Young; Lee, Jehee

    2010-12-01

    The regulation of transcriptional activation is an essential and critical point in gene expression. In this study, we describe a novel transcription factor activator protein-1 (Ap-1) gene from disk abalone Haliotis discus discus (AbAp-1) for the first time in mollusk. It was identified by homology screening of an abalone normalized cDNA library. The cloned AbAp-1 consists of a 945 bp coding region that encodes a putative protein containing 315 amino acids. The AbAp-1 gene is composed of a characteristic Jun transcription factor domain and a highly conserved basic leucine zipper (bZIP) signature similar to known Ap-1 genes. The AbAp-1 shares 46, 43 and, 40% amino acid identities with fish (Takifugu rubripes), human and insect (Ixodes scapularis) Ap-1, respectively. Quantitative real time RT-PCR analysis confirmed that AbAp-1 gene expression is constitutive in all selected tissues. AbAp-1 was upregulated in gills after bacteria (Vibrio alginolyticus, Vibrio parahemolyticus and Lysteria monocytogenes) challenge; and, upregulated in hemocytes and gills by viral hemorrhagic septicemia virus (VHSV) challenge. Shell damage and tissue injury also increased the transcriptional level of Ap-1 in mantle together with other transcription factors (NF-kB, LITAF) and pro-inflammatory TNF-α. All results considered, identification and gene expression data demonstrate that abalone Ap-1 is an important regulator in innate immune response against bacteria and virus, as well as in the inflammatory response during tissue injury. In addition, stimulation of Ap-1 under different external stimuli could be useful to understand the Ap-1 biology and its downstream target genes, especially in abalone-like mollusks.

  14. AP600 containment purge radiological analysis

    Energy Technology Data Exchange (ETDEWEB)

    O`Connor, M.; Schulz, J.; Tan, C. [Bechtel Power Corporation (United States)] [and others

    1995-02-01

    The AP600 Project is a passive pressurized water reactor power plant which is part of the Design Certification and First-of-a-Kind Engineering effort under the Advanced Light Water Reactor program. Included in this process is the design of the containment air filtration system which will be the subject of this paper. We will compare the practice used by previous plants with the AP600 approach to meet the goals of industry standards in sizing the containment air filtration system. The radiological aspects of design are of primary significance and will be the focus of this paper. The AP600 Project optimized the design to combine the functions of the high volumetric flow rate, low volumetric flow rate, and containment cleanup and other filtration systems into one multi-functional system. This achieves a more simplified, standardized, and lower cost design. Studies were performed to determine the possible concentrations of radioactive material in the containment atmosphere and the effectiveness of the purge system to keep concentrations within 10CFR20 limits and within offsite dose objectives. The concentrations were determined for various reactor coolant system leakage rates and containment purge modes of operation. The resultant concentrations were used to determine the containment accessibility during various stages of normal plant operation including refueling. The results of the parametric studies indicate that a dual train purge system with a capacity of 4,000 cfm per train is more than adequate to control the airborne radioactivity levels inside containment during normal plant operation and refueling, and satisfies the goals of ANSI/ANS-56.6-1986 and limits the amount of radioactive material released to the environment per ANSI/ANS 59.2-1985 to provide a safe environment for plant personnel and offsite residents.

  15. Ozone mitigation tests at the APS

    Energy Technology Data Exchange (ETDEWEB)

    Kuzay, T.M.; Collins, J.T.; Pisharody, M.; Job, P.K.; Wang Zhibi

    1996-09-01

    Ozone is generated in the APS experimental stations whenever the x-ray beam has a chance to interact with air. Ozone concentrations in an experimental station have to be below a certain defined limit (current OSHA regulations specify 0.08 ppm as the maximum limit) before an experimenter can reenter the hutch. This limit is said to be currently under study for a downward adjustment. One method of depleting the ozone generated in an experimental station is mitigation through either adsorption or direct destruction. In recent tests, both methods were tried using commercially available units. Test results and some analytical predictions are presented.

  16. Wiley AP english language and composition

    CERN Document Server

    Woods, Geraldine

    2013-01-01

    Two full-length practice tests to help you score your highest Review questions with complete answers and explanations for the most popular themes on the test Study strategies and expert test-taking tips to keep you calm and focused on test day Review key exam topics and themes to maximize your score Preparing for the AP English Language and Composition test? This test-prep guide helps you perfect the skills you need to achieve your best possible score. You'll find winning test-taking tips, multiple-choice strategies, and essay guidelines, as well as great advice on getting the

  17. Cloning and characterisation of hAps1 and hAps2, human diadenosine polyphosphate-metabolising Nudix hydrolases

    Directory of Open Access Journals (Sweden)

    Safrany Stephen T

    2002-07-01

    Full Text Available Abstract Background The human genome contains at least 18 genes for Nudix hydrolase enzymes. Many have similar functions to one another. In order to understand their roles in cell physiology, these proteins must be characterised. Results We have characterised two novel human gene products, hAps1, encoded by the NUDT11 gene, and hAps2, encoded by the NUDT10 gene. These cytoplasmic proteins are members of the DIPP subfamily of Nudix hydrolases, and differ from each other by a single amino acid. Both metabolise diadenosine-polyphosphates and, weakly, diphosphoinositol polyphosphates. An apparent polymorphism of hAps1 has also been identified, which leads to the point mutation S39N. This has also been characterised. The favoured nucleotides were diadenosine 5',5"'-pentaphosphate (kcat/Km = 11, 8 and 16 × 103M-1s-1 respectively for hAps1, hAps1-39N and hAps2 and diadenosine 5',5"'-hexaphosphate (kcat/Km = 13, 14 and 11 × 103M-1s-1 respectively for hAps1, hAps1-39N and hAps2. Both hAps1 and hAps2 had pH optima of 8.5 and an absolute requirement for divalent cations, with manganese (II being favoured. Magnesium was not able to activate the enzymes. Therefore, these enzymes could be acutely regulated by manganese fluxes within the cell. Conclusions Recent gene duplication has generated the two Nudix genes, NUDT11 and NUDT10. We have characterised their gene products as the closely related Nudix hydrolases, hAps1 and hAps2. These two gene products complement the activity of previously described members of the DIPP family, and reinforce the concept that Ap5A and Ap6A act as intracellular messengers.

  18. SN 2005ap: A Most Brilliant Explosion

    CERN Document Server

    Quimby, Robert M; Wheeler, J Craig; Höflich, Peter; Akerlof, Carl W; Rykoff, Eli S

    2007-01-01

    We present unfiltered photometric observations with ROTSE-III and optical spectroscopic follow-up with the HET and Keck of the most luminous supernova yet identified, SN 2005ap. The spectra taken about 3 days before and 6 days after maximum light show narrow emission lines (likely originating in the dwarf host) and absorption lines at a redshift of z=0.2832, which puts the peak unfiltered magnitude at -22.7 +/- 0.1 absolute. Broad P-Cygni features corresponding to H-alpha, CIII, NIII, and OIII, are further detected with a photospheric velocity of ~20,000 km/s. Unlike other highly luminous supernovae such as 2006gy and 2006tf that show slow photometric evolution, the light curve of SN 2005ap indicates a 1-3 week rise to peak followed by a relatively rapid decay. The spectra also lack the distinct emission peaks from moderately broadened (FWHM ~ 2,000 km/s) Balmer lines seen in SN 2006gy and SN 2006tf. We briefly discuss the origin of the extraordinary luminosity from a strong interaction as may be expected fro...

  19. The APS control system network upgrade.

    Energy Technology Data Exchange (ETDEWEB)

    Sidorowicz, K. v.; Leibfritz, D.; McDowell, W. P.

    1999-10-22

    When it was installed,the Advanced Photon Source (APS) control system network was at the state-of-the-art. Different aspects of the system have been reported at previous meetings [1,2]. As loads on the controls network have increased due to newer and faster workstations and front-end computers, we have found performance of the system declining and have implemented an upgraded network. There have been dramatic advances in networking hardware in the last several years. The upgraded APS controls network replaces the original FDDI backbone and shared Ethernet hubs with redundant gigabit uplinks and fully switched 10/100 Ethernet switches with backplane fabrics in excess of 20 Gbits/s (Gbps). The central collapsed backbone FDDI concentrator has been replaced with a Gigabit Ethernet switch with greater than 30 Gbps backplane fabric. Full redundancy of the system has been maintained. This paper will discuss this upgrade and include performance data and performance comparisons with the original network.

  20. The Electronic ApJ in Context

    Science.gov (United States)

    Biemesderfer, C. D.

    1995-12-01

    The on-line edition of the Astrophysical Journal provides the first opportunity for astronomical researchers to interact with the literature in a network context. Other electronic journals will follow. Astronomers will continue to develop ways to take advantage of information on the network, and these will be most effective when ApJ articles, observations from data centers, proposals for telescope time, communications with collaborators, and so on, are meshed. Astronomical literature, as a resource on the Internet, is not unique from the viewpoint of function or management, and astronomers are not unique as scholars in their desire to use the Internet as a tool to advance their work. We need to ensure as a community that all our resources for research --- journals and data --- fit in a global context on an Internet being used by everyone. In other words, we have to be sure that the ApJ fits into all the digital libraries of the future, including the ones we don't build. This paper will present a scenario in which many varied forms of information, astronomical and otherwise, can be catalogued and distributed so that they can be discovered and used by the research community.

  1. Evaluating Peer-Led Team Learning across the Two Semester General Chemistry Sequence

    Science.gov (United States)

    Mitchell, Yancey D.; Ippolito, Jessica; Lewis, Scott E.

    2012-01-01

    Peer-Led Team Learning (PLTL) is a widely disseminated pedagogical reform that employs previously successful undergraduate students, peer leaders, to lead sessions of structured group work in the target class. Numerous studies have evaluated the impact of this reform in various post-secondary chemistry classes. Results from these studies suggest…

  2. Cost-Savings Achieved in Two Semesters Through the Adoption of Open Educational Resources

    Directory of Open Access Journals (Sweden)

    John Levi Hilton III

    2014-04-01

    Full Text Available Textbooks represent a significant portion of the overall cost of higher education in the United States. The burden of these costs is typically shouldered by students, those who support them, and the taxpayers who fund the grants and student loans which pay for textbooks. Open educational resources (OER provide students a way to receive high-quality learning materials at little or no cost to students. We report on the cost savings achieved by students at eight colleges when these colleges began utilizing OER in place of traditional commercial textbooks.

  3. Cost-Savings Achieved in Two Semesters through the Adoption of Open Educational Resources

    Science.gov (United States)

    Hilton, John Levi, III; Robinson, Jared; Wiley, David; Ackerman, J. Dale

    2014-01-01

    Textbooks represent a significant portion of the overall cost of higher education in the United States. The burden of these costs is typically shouldered by students, those who support them, and the taxpayers who fund the grants and student loans which pay for textbooks. Open educational resources (OER) provide students a way to receive…

  4. Evaluating Peer-Led Team Learning across the Two Semester General Chemistry Sequence

    Science.gov (United States)

    Mitchell, Yancey D.; Ippolito, Jessica; Lewis, Scott E.

    2012-01-01

    Peer-Led Team Learning (PLTL) is a widely disseminated pedagogical reform that employs previously successful undergraduate students, peer leaders, to lead sessions of structured group work in the target class. Numerous studies have evaluated the impact of this reform in various post-secondary chemistry classes. Results from these studies suggest…

  5. AP1000进度管理研究%The study on AP1000 Schedule management

    Institute of Scientific and Technical Information of China (English)

    李阳; 王飞鸣

    2015-01-01

    核电建设进度管理非常重要,本文针对核电站进度管理的内容、体系、管理方式等进行介绍,并结合AP1000核电技术的特点——模块化,分析其对进度管理的影响和目前存在的问题。并结合影响和问题提出了在后续AP1000建设进度管理中的几项建议。%Nuclear power plant construction progress management is very important. This paper addresses important topics including the content, system and manage methods of nuclear power plants construction. Combining the characteristics of AP1000 nuclear power technology-modular, the paper analyzed its impact on progress management and the existing problems. Depending on the impacts and problems, the paper also raised several suggestions in AP1000 modular construction.

  6. Trichomonas vaginalis: the adhesins AP51 and AP65 bind heme and hemoglobin.

    Science.gov (United States)

    Ardalan, Shahed; Lee, B Craig; Garber, Gary E

    2009-04-01

    Trichomonas vaginalis is the cause of human trichomoniasis, the most common non-viral sexually transmitted disease worldwide. Although acquisition of iron by binding to host hemoglobin through distinct receptor(s) has been described, no specific heme- or hemoglobin-binding site has been reported in this parasite. To determine the presence of hemoglobin-binding protein(s), membrane proteins were subjected to hemoglobin-affinity chromatography. Eluted proteins were analysed by SDS-PAGE. Two protein bands of 48 and 63 kDa were detected. Competition assay with an excess amount of hemoglobin or hemin in hemoglobin-affinity chromatography could block the 63- and 48-kDa bands, respectively. Further analysis by mass spectrometry indicated that the 48- and 63-kDa proteins had identity with two T. vaginalis adhesins: AP51 and AP65, respectively. This study confirms the existence of multifunctional proteins in T. vaginalis, and suggested that AP51 and AP65, besides serving as adhesion molecules, could also act as heme- and hemoglobin-binding proteins.

  7. Specific activation of human interleukin-5 depends on de novo synthesis of an AP-1 complex.

    Science.gov (United States)

    Schwenger, Gretchen T F; Kok, Chee Choy; Arthaningtyas, Estri; Thomas, Marc A; Sanderson, Colin J; Mordvinov, Viatcheslav A

    2002-12-06

    It is clear from the biology of eosinophilia that a specific regulatory mechanism must exist. Because interleukin-5 (IL5) is the key regulatory cytokine, it follows that a gene-specific control of IL5 expression must exist that differs even from closely related cytokines such as IL4. Two features of IL5 induction make it unique compared with other cytokines; first, induction by cyclic adenosine monophosphate (cAMP), which inhibits other T-cell-derived cytokines, and second, sensitivity to protein synthesis inhibitors, which have no effect on other cytokines. This study has utilized the activation of different transcription factors by different stimuli in a human T-cell line to study the role of conserved lymphokine element 0 (CLE0) in the specific induction of IL5. In unstimulated cells the ubiquitous Oct-1 binds to CLE0. Stimulation induces de novo synthesis of the AP-1 members JunD and Fra-2, which bind to CLE0. The amount of IL5 produced correlates with the production of the AP-1 complex, suggesting a key role in IL5 expression. The formation of the AP-1 complex is essential, but the rate-limiting step is the synthesis of AP-1, especially Fra-2. This provides an explanation for the sensitivity of IL5 to protein synthesis inhibitors and a mechanism for the specific induction of IL5 compared with other cytokines.

  8. Proximal femoral canal shape is more accurately assessed on AP hip radiographs than AP pelvis radiographs in primary hip osteoarthritis.

    Science.gov (United States)

    Merle, Christian; Waldstein, Wenzel; Gregory, Jennifer S; Goodyear, Simon R; Aspden, Richard M; Aldinger, Peter R; Murray, David W; Gill, Harinderjit S

    2013-01-01

    The objectives of the present study were to determine whether differences in the radiographic appearance of the of the proximal femoral canal exist on corresponding AP pelvis and AP hip radiographs, and whether radiographic assessment of canal shape is accurate with reference to computed tomography (CT). In a retrospective study, corresponding radiographs and CT scans of 100 consecutive patients with primary hip OA were evaluated. Active shape modelling (ASM) was performed to assess the variation in proximal femoral canal shape and to identify differences between AP hip and AP pelvis views. Differences in the medial cortical flare between radiographs and CT were quantified using least squares curve fitting. ASM identified significant differences in the assessment of canal shape on corresponding AP hip and AP pelvis views. Curve fitting demonstrated a good agreement between AP hip radiographs and CT. Agreement between AP pelvis radiographs and CT was less good. In contrast to AP pelvis radiographs, AP hip radiographs allow a more accurate and reliable assessment of proximal femoral canal shape in the frontal plane in primary hip OA. Our findings may improve stem fit in total hip arthroplasty without the routine use of CT.

  9. Low Earth orbit assessment of proton anisotropy using AP8 and AP9 trapped proton models.

    Science.gov (United States)

    Badavi, Francis F; Walker, Steven A; Santos Koos, Lindsey M

    2015-04-01

    The completion of the International Space Station (ISS) in 2011 has provided the space research community with an ideal evaluation and testing facility for future long duration human activities in space. Ionized and secondary neutral particles radiation measurements inside ISS form the ideal tool for validation of radiation environmental models, nuclear reaction cross sections and transport codes. Studies using thermo-luminescent detectors (TLD), tissue equivalent proportional counter (TPEC), and computer aided design (CAD) models of early ISS configurations confirmed that, as input, computational dosimetry at low Earth orbit (LEO) requires an environmental model with directional (anisotropic) capability to properly describe the exposure of trapped protons within ISS. At LEO, ISS encounters exposure from trapped electrons, protons and geomagnetically attenuated galactic cosmic rays (GCR). For short duration studies at LEO, one can ignore trapped electrons and ever present GCR exposure contributions during quiet times. However, within the trapped proton field, a challenge arises from properly estimating the amount of proton exposure acquired. There exist a number of models to define the intensity of trapped particles. Among the established trapped models are the historic AE8/AP8, dating back to the 1980s and the recently released AE9/AP9/SPM. Since at LEO electrons have minimal exposure contribution to ISS, this work ignores the AE8 and AE9 components of the models and couples a measurement derived anisotropic trapped proton formalism to omnidirectional output from the AP8 and AP9 models, allowing the assessment of the differences between the two proton models. The assessment is done at a target point within the ISS-11A configuration (circa 2003) crew quarter (CQ) of Russian Zvezda service module (SM), during its ascending and descending nodes passes through the south Atlantic anomaly (SAA). The anisotropic formalism incorporates the contributions of proton narrow

  10. Low Earth orbit assessment of proton anisotropy using AP8 and AP9 trapped proton models

    Science.gov (United States)

    Badavi, Francis F.; Walker, Steven A.; Santos Koos, Lindsey M.

    2015-04-01

    The completion of the International Space Station (ISS) in 2011 has provided the space research community with an ideal evaluation and testing facility for future long duration human activities in space. Ionized and secondary neutral particles radiation measurements inside ISS form the ideal tool for validation of radiation environmental models, nuclear reaction cross sections and transport codes. Studies using thermo-luminescent detectors (TLD), tissue equivalent proportional counter (TPEC), and computer aided design (CAD) models of early ISS configurations confirmed that, as input, computational dosimetry at low Earth orbit (LEO) requires an environmental model with directional (anisotropic) capability to properly describe the exposure of trapped protons within ISS. At LEO, ISS encounters exposure from trapped electrons, protons and geomagnetically attenuated galactic cosmic rays (GCR). For short duration studies at LEO, one can ignore trapped electrons and ever present GCR exposure contributions during quiet times. However, within the trapped proton field, a challenge arises from properly estimating the amount of proton exposure acquired. There exist a number of models to define the intensity of trapped particles. Among the established trapped models are the historic AE8/AP8, dating back to the 1980s and the recently released AE9/AP9/SPM. Since at LEO electrons have minimal exposure contribution to ISS, this work ignores the AE8 and AE9 components of the models and couples a measurement derived anisotropic trapped proton formalism to omnidirectional output from the AP8 and AP9 models, allowing the assessment of the differences between the two proton models. The assessment is done at a target point within the ISS-11A configuration (circa 2003) crew quarter (CQ) of Russian Zvezda service module (SM), during its ascending and descending nodes passes through the south Atlantic anomaly (SAA). The anisotropic formalism incorporates the contributions of proton narrow

  11. The Cryptosporidium parvum ApiAP2 gene family: insights into the evolution of apicomplexan AP2 regulatory systems.

    Science.gov (United States)

    Oberstaller, Jenna; Pumpalova, Yoanna; Schieler, Ariel; Llinás, Manuel; Kissinger, Jessica C

    2014-07-01

    We provide the first comprehensive analysis of any transcription factor family in Cryptosporidium, a basal-branching apicomplexan that is the second leading cause of infant diarrhea globally. AP2 domain-containing proteins have evolved to be the major regulatory family in the phylum to the exclusion of canonical regulators. We show that apicomplexan and perkinsid AP2 domains cluster distinctly from other chromalveolate AP2s. Protein-binding specificity assays of C. parvum AP2 domains combined with motif conservation upstream of co-regulated gene clusters allowed the construction of putative AP2 regulons across the in vitro life cycle. Orthologous Apicomplexan AP2 (ApiAP2) expression has been rearranged relative to the malaria parasite P. falciparum, suggesting ApiAP2 network rewiring during evolution. C. hominis orthologs of putative C. parvum ApiAP2 proteins and target genes show greater than average variation. C. parvum AP2 domains display reduced binding diversity relative to P. falciparum, with multiple domains binding the 5'-TGCAT-3', 5'-CACACA-3' and G-box motifs (5'-G[T/C]GGGG-3'). Many overrepresented motifs in C. parvum upstream regions are not AP2 binding motifs. We propose that C. parvum is less reliant on ApiAP2 regulators in part because it utilizes E2F/DP1 transcription factors. C. parvum may provide clues to the ancestral state of apicomplexan transcriptional regulation, pre-AP2 domination. © The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

  12. Market analysis of APS/SCM applications and technologies

    OpenAIRE

    Prášil, Zdeněk

    2009-01-01

    The bachelor thesis aims to describe a current worldwide market of APS/SCM technologies and applications , i.e. advanced planned scheduling /supply chain management. In the first part, the APS/SCM is described in theory. The APS/SCM is defined and its benefits and impacts on company are discussed. The next part of the work is focused on the market with APS/SCM and the distribution of forces in the market. The demand and supply of this market segment is analyzed. In the last part, solutions of...

  13. ONLINE MINIMIZATION OF VERTICAL BEAM SIZES AT APS

    Energy Technology Data Exchange (ETDEWEB)

    Sun, Yipeng

    2017-06-25

    In this paper, online minimization of vertical beam sizes along the APS (Advanced Photon Source) storage ring is presented. A genetic algorithm (GA) was developed and employed for the online optimization in the APS storage ring. A total of 59 families of skew quadrupole magnets were employed as knobs to adjust the coupling and the vertical dispersion in the APS storage ring. Starting from initially zero current skew quadrupoles, small vertical beam sizes along the APS storage ring were achieved in a short optimization time of one hour. The optimization results from this method are briefly compared with the one from LOCO (Linear Optics from Closed Orbits) response matrix correction.

  14. Project W-211, initial tank retrieval systems, description of operations for 241-AP-102 and 241-AP-104

    Energy Technology Data Exchange (ETDEWEB)

    RIECK, C.A.

    1999-02-25

    The primary purpose of the Initial Tank Retrieval Systems (ITRS) is to provide systems for retrieval of radioactive wastes stored in underground double-shell tanks (DSTS) for transfer to alternate storage, evaporation, pretreatment or treatment, while concurrently reducing risks associated with safety watch list and other DSTs. This Description of Operations (DOO) defines the control philosophy for the waste retrieval system for tanks 241-AP-102 (AP-102) and 241-AP-104 (AP-104). This DOO will provide a basis for the detailed design of the Retrieval Control System (RCS) for AP-102 and AP-104 and establishes test criteria for the RCS. The test criteria will be used during qualification testing and acceptance testing to verify operability.

  15. AP1000主系统先进性分析%The Progressiveness Analysis of the AP1000 Main System

    Institute of Scientific and Technical Information of China (English)

    李家杰

    2012-01-01

      本文总结了第三代先进非能动压水堆核电厂AP1000主系统的主要特点,对AP1000主系统的先进性进行了分析,核电厂主系统设计人员能够从该文中了解到 AP1000主系统与第二代核电厂主系统之间的差别。%  This paper summarizes the main features of the AP1000 main system of the third generation of advanced passive pressurized water reactor nuclear power plant , and analyzes the progressiveness of the AP1000 main system .From this paper, the main system designers of nuclear power factory can learn the difference between the second-generation nuclear power factory main system and AP1000 main system

  16. Project W-211, initial tank retrieval systems, description of operations for 241-AP-102 and 241-AP-104

    Energy Technology Data Exchange (ETDEWEB)

    RIECK, C.A.

    1999-02-25

    The primary purpose of the Initial Tank Retrieval Systems (ITRS) is to provide systems for retrieval of radioactive wastes stored in underground double-shell tanks (DSTS) for transfer to alternate storage, evaporation, pretreatment or treatment, while concurrently reducing risks associated with safety watch list and other DSTs. This Description of Operations (DOO) defines the control philosophy for the waste retrieval system for tanks 241-AP-102 (AP-102) and 241-AP-104 (AP-104). This DOO will provide a basis for the detailed design of the Retrieval Control System (RCS) for AP-102 and AP-104 and establishes test criteria for the RCS. The test criteria will be used during qualification testing and acceptance testing to verify operability.

  17. Cell-based Fluorescence Complementation Reveals a Role for HIV-1 Nef Protein Dimerization in AP-2 Adaptor Recruitment and CD4 Co-receptor Down-regulation.

    Science.gov (United States)

    Shu, Sherry T; Emert-Sedlak, Lori A; Smithgall, Thomas E

    2017-02-17

    The HIV-1 Nef accessory factor enhances viral infectivity, immune evasion, and AIDS progression. Nef triggers rapid down-regulation of CD4 via the endocytic adaptor protein 2 (AP-2) complex, a process linked to enhanced viral infectivity and immune escape. Here, we describe a bimolecular fluorescence complementation (BiFC) assay to visualize the interaction of Nef with AP-2 and CD4 in living cells. Interacting protein pairs were fused to complementary non-fluorescent fragments of YFP and co-expressed in 293T cells. Nef interactions with both CD4 and AP-2 resulted in complementation of YFP and a bright fluorescent signal by confocal microcopy that localized to the cell periphery. Co-expression of the AP-2 α subunit enhanced the Nef·AP-2 σ2 subunit BiFC signal and vice versa, suggesting that the AP-2 α-σ2 hemicomplex interacts cooperatively with Nef. Mutagenesis of Nef amino acids Arg-134, Glu-174, and Asp-175, which stabilize Nef for AP-2 α-σ2 binding in a recent co-crystal structure, substantially reduced AP-2 interaction without affecting CD4 binding. A dimerization-defective mutant of Nef failed to interact with either CD4 or AP-2 in the BiFC assay, indicating that Nef quaternary structure is required for CD4 and AP-2 recruitment as well as CD4 down-regulation. A small molecule previously shown to bind the Nef dimerization interface also reduced Nef interactions with AP-2 and CD4 and restored CD4 expression to the surface of HIV-infected cells. Our findings provide a mechanistic explanation for previous observations that dimerization-defective Nef mutants fail to down-regulate CD4 and validate the Nef dimerization interface as a target site for antiretroviral drug development. © 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

  18. Top-up operation experience at APS.

    Energy Technology Data Exchange (ETDEWEB)

    Emery, L.

    1999-03-31

    The Advanced Photon Source (APS) is a 7-OeV, third-generation synchrotrons radiation source. To provide more stable beam for users, in September 1998 we began commissioning a new operating mode called ''top-up.'' In this mode, the beam current does not decay but is maintained at a high level using frequent injection, while photon shutters are open and photon beams are delivered to users. The hardware, software, and safety requirements for top-up will be reported. Safety issues related to injection with open photon shutters are covered in companion papers in this conference. Recent operational experience includes testing aspects of top-up injection and delivering beam to X-ray users for a few hours with fractional current stability of 10{sup {minus}3}. We expect to run several top-up operation shifts in Spring 1999. Issues of importance are orbit and emittance transients during the injection and scheduling of injection pulses for the convenience of users.

  19. APS extends open access to all its journals

    CERN Multimedia

    Thomas, Kim

    2006-01-01

    "Physics research promoter and publisher the American Physical Society (APS) is to extend open access to all its journals. Th APS previously made its five print journals available through subscriptions, and its two e-journals (Physical Review Special Topics and Physics Educatoin Research) on an open access basis." (1/2 page)

  20. AP233: An Information Model for Systems Engineering

    Science.gov (United States)

    Siebes, Georg

    2009-01-01

    In today's world, information is abundant. We have no problems generating it. But we are challenged to find, organize, and exchange information. center dot A standardized model of information can help. Such a model nearly completed its development for Systems Engineering. It is referred to as AP233 (AP = Application Protocol).

  1. Properties of LuAP: CE scintillator containing intentional impurities

    CERN Document Server

    Petrosyan, A G; Ovanesyan, K; Lecoq, Paul; Auffray, Etiennette; Trummer, Julia; Kronberger, Matthias; Pédrini, C; Dujardin, C; Anfre, P

    2007-01-01

    Single crystals of LuAP:Ce and LuYAP(Lu*70%):Ce co-doped with tetravalent (Hf and Zr) and pentavalent (Ta) ions were grown from melts by the Bridgman process. Underlying absorption, slope of the optical edge and transmission in the range of emission were compared to those of LuAP:Ce crystals. Absorption coefficients at 260 nm less than 2 cm−1 have been recorded in LuAP:Ce crystals containing tetravalent ions that are lower than the corresponding figures (5–6 cm−1) measured in undoped LuAP. At high concentrations of added impurities, despite of suppression of the parasitic underlying absorption below 300 nm, the slope of the optical edge and transmission in the range of emission are seriously damaged. Scintillation parameters of crystals with added impurities are compared to those of LuAP:Ce.

  2. Stratification in Ap star atmospheres: Simulations

    Science.gov (United States)

    Cowley, Charles R.; Castelli, Fiorella

    2017-01-01

    It is now well established that the atmospheres of Ap stars can be chemically stratified (cf. Babel, A\\&A 258, 645, 1992; Ryabchikova et al. A\\&A 384, 545, 2002). The most convincing cases have been made with the profiles of very strong lines, such as Ca II K. Weaker line profiles are less obvious indicators. The collective behavior of sets or groups of lines have also been used. For example, if higher abundances are derived for strong lines in an atmosphere with zero microturbulence, one may assume that the absorbing species has been pushed into the higher photospheres. An example are the medium-strong Mn II lines in HgMn stars. In this paper, we probe this assumption by calculating line strengths with various assumed stratification models, and then determining abundances from those lines using an {\\bf unstratified} model with the same Teff and log(g). We use the model from Castelli, Kurucz \\& and Hubrig (A\\&A, 508, 401, 2009) for HR 6000, whose spectrum shows numerous indications of stratification. A variety of stratification models are considered, for example, ones where the majority of an absorbing species is concentrated above (or below) $log(\\tau_{5000}$ = -2.0. Cloud models are also investigated, where a species is concentrated within a range of photospheric depths. Curves of growth are generated in unstratified atmospheres for lines by holding the abundance fixed, and increasing log(gf). Similar curves are made in stratified models, and the ratios of strong to weak linesare compared with and without stratification. The effects of stratification on ionization are also investigated, as well as on the profiles of strong lines. We find, in agreement with previous work, that severe abundance jumps are sometimes required to account for some of the observed peculiarities.

  3. Thermal Decomposition Characteristics of Orthorhombic Ammonium Perchlorate (o-AP) and an 0-AP/HTPB-Based Propellant

    Energy Technology Data Exchange (ETDEWEB)

    BEHRENS JR.,RICHARD; MINIER,LEANNA M.G.

    1999-10-25

    A study to characterize the low-temperature reactive processes for o-AP and an AP/HTPB-based propellant (class 1.3) is being conducted in the laboratory using the techniques of simultaneous thermogravimetric modulated beam mass spectrometry (STMBMS) and scanning electron microscopy (SEM). The results presented in this paper are a follow up of the previous work that showed the overall decomposition to be complex and controlled by both physical and chemical processes. The decomposition is characterized by the occurrence of one major event that consumes up to {approx}35% of the AP, depending upon particle size, and leaves behind a porous agglomerate of AP. The major gaseous products released during this event include H{sub 2}O, O{sub 2}, Cl{sub 2}, N{sub 2}O and HCl. The recent efforts provide further insight into the decomposition processes for o-AP. The temporal behaviors of the gas formation rates (GFRs) for the products indicate that the major decomposition event consists of three chemical channels. The first and third channels are affected by the pressure in the reaction cell and occur at the surface or in the gas phase above the surface of the AP particles. The second channel is not affected by pressure and accounts for the solid-phase reactions characteristic of o-AP. The third channel involves the interactions of the decomposition products with the surface of the AP. SEM images of partially decomposed o-AP provide insight to how the morphology changes as the decomposition progresses. A conceptual model has been developed, based upon the STMBMS and SEM results, that provides a basic description of the processes. The thermal decomposition characteristics of the propellant are evaluated from the identities of the products and the temporal behaviors of their GFRs. First, the volatile components in the propellant evolve from the propellant as it is heated. Second, the hot AP (and HClO{sub 4}) at the AP-binder interface oxidize the binder through reactions that

  4. Authenticated, private, and secured smart cards (APS-SC)

    Science.gov (United States)

    Szu, Harold; Mehmood, Amir

    2006-04-01

    From historical perspective, the recent advancements in better antenna designs, low power circuitry integrations and inexpensive fabrication materials have made possible a miniature counter-measure against Radar, a clutter behaving like a fake target return called Digital Reflection Frequency Modulation (DRFM). Such a military counter-measure have found its way in the commerce as a near field communication known as Radio Frequency Identification (RFID), a passive or active item tag T attached to every readable-writable Smart Card (SC): Passports ID, medical patient ID, biometric ID, driver licenses, book ID, library ID, etc. These avalanche phenomena may be due to the 3 rd Gen phones seeking much more versatile & inexpensive interfaces, than the line-of-sight bar-code optical scan. Despite of the popularity of RFID, the lacking of Authenticity, Privacy and Security (APS) protection restricted somewhat the wide spread commercial, financial, medical, legal, and militarily applications. Conventional APS approach can obfuscate a private passkey K of SC with the tag number T or the reader number R, or both, i.e. only T*K or R*K or both will appear on them, where * denotes an invertible operation, e.g. EXOR, but not limited to it. Then, only the authentic owner, knowing all, can inverse the operation, e.g. EXOR*EXOR= I to find K. However, such an encryption could be easily compromised by a hacker seeking exhaustively by comparison based on those frequently used words. Nevertheless, knowing biological wetware lesson for power of pairs sensors and Radar hardware counter-measure history, we can counter the counter-measure DRFM, instead using one RFID tag per SD, we follow the Nature adopting two ears/tags, e.g. each one holding portions of the ID or simply two different ID's readable only by different modes of the interrogating reader, followed by brain central processor in terms of nonlinear invertible shufflers mixing two ID bits. We prefer to adopt such a hardware

  5. MULTI-OBJECTIVE ONLINE OPTIMIZATION OF BEAM LIFETIME AT APS

    Energy Technology Data Exchange (ETDEWEB)

    Sun, Yipeng

    2017-06-25

    In this paper, online optimization of beam lifetime at the APS (Advanced Photon Source) storage ring is presented. A general genetic algorithm (GA) is developed and employed for some online optimizations in the APS storage ring. Sextupole magnets in 40 sectors of the APS storage ring are employed as variables for the online nonlinear beam dynamics optimization. The algorithm employs several optimization objectives and is designed to run with topup mode or beam current decay mode. Up to 50\\% improvement of beam lifetime is demonstrated, without affecting the transverse beam sizes and other relevant parameters. In some cases, the top-up injection efficiency is also improved.

  6. AP600 design certification thermal hydraulics testing and analysis

    Energy Technology Data Exchange (ETDEWEB)

    Hochreiter, L.E.; Piplica, E.J.

    1995-09-01

    Westinghouse Electric Corporation, in conjunction with the Department of Energy and the Electric Power Research Institute, have been developing an advanced light water reactor design; the AP600. The AP600 is a 1940 Mwt, 600Mwe unit which is similar to a Westinghouse two-loop Pressurized Water Reactor. The accumulated knowledge on reactor design to reduce the capital costs, construction time, and the operational and maintenance cost of the unit once it begins to generate electrical power. The AP600 design goal is to maintain an overall cost advantage over fossil generated electrical power.

  7. A new gap separation mechanism for APS insertion devices.

    Energy Technology Data Exchange (ETDEWEB)

    Trakhtenberg, E. M.; Tcheskidov, V.; Den Hartog, P. K.; Deriy, B.; Erdmann, M.; Makarov, O.; Moog, E. R.

    1999-10-25

    A new gap separation mechanism for use with the standard Advanced Photon Source (APS) 3.3-cm-period undulator magnetic structures has been designed and built and the first system has been installed in the APS storage ring. The system allows a minimum magnetic gap of 10 mm for use with the APS 8-mm insertion device vacuum chambers. The mechanism is a bolted steel frame structure with a simple 4-motor mechanical drive train. The control system uses servomotors with incremental rotary encoders and virtual absolute linear encoders.

  8. Mudança no padrão biológico da migrânea com aura após a utilização da tetrabenazina: relato de caso Change in the biologic pattern of the migraine with aura after the use of tetrabenazine: case report

    Directory of Open Access Journals (Sweden)

    ELCIO JULIATO PIOVESAN

    2000-06-01

    Full Text Available A participação do sistema dopaminérgico na fisiopatologia da migrânea tem sido proposta a partir de recentes conquistas genéticas. Uma possível hipereatividade dos receptores dopaminérgicos DRD2 tornou as evidências mais contundentes neste sentido. Descrevemos paciente masculino, 31 anos, portador de distonia generalizada, secundária a hipóxia perinatal. Aos 16 anos, começou a ter crises de cefaléia que preenchiam os critérios para migrânea com aura. Três anos após tratamento da distônia com tetrabenazina, observou-se nítida redução da frequência, intensidade e duração das crises. Durante dois períodos, superiores a dois meses, a interrupção do tratamento com tetrabenazina induziu piora nas crises de migrânea. Apresentamos este relato como sendo a primeira descrição na literatura mostrando efeitos benéficos da tetrabenazina, um bloqueador dos receptores dopaminérgicos, sobre o comportamento da migrânea com aura.The influence of the dopaminergic system on the pathophysiology of migraine has been suggested as a result of recent genetic discoveries. A possible hyper-reactivity of the dopaminergic receptors DRD2 reinforced the evidence regarding this. We describe a 31 years-old male patient affected by a generalized dystonia secondary to perinatal hypoxia. At age 16, the patient started having headache crises that met the criteria for migraine with aura. After three years of treatment for dystonia with tetrabenazine, a clear reduction in the frequency, intensity and duration of the crises was perceived. During two periods longer than two months, the interruption of the treatment with tetrabenazine brought about an aggravation of the migraine crises. We present this case as being the first description in the literature showing the beneficial effects of tetrabenazine, a blocker of dopaminergic receptors, on the behavior of migraine with aura.

  9. Human Immunodeficiency Virus Type 2 (HIV-2 Gag Is Trafficked in an AP-3 and AP-5 Dependent Manner.

    Directory of Open Access Journals (Sweden)

    Justine E Alford

    Full Text Available Although human immunodeficiency virus (HIV types 1 and 2 are closely related lentiviruses with similar replication cycles, HIV-2 infection is associated with slower progression to AIDS, a higher proportion of long term non-progressors, and lower rates of transmission than HIV-1, likely as a consequence of a lower viral load during HIV-2 infection. A mechanistic explanation for the differential viral load remains unclear but knowledge of differences in particle production between HIV-1 and HIV-2 may help to shed light on this issue. In contrast to HIV-1, little is known about the assembly of HIV-2 particles, and the trafficking of HIV-2 Gag, the structural component of the virus, within cells. We have established that HIV-2 Gag accumulates in intracellular CD63 positive compartments, from which it may be delivered or recycled to the cell surface, or degraded. HIV-2 particle release was dependent on the adaptor protein complex AP-3 and the newly identified AP-5 complex, but much less so on AP-1. In contrast, HIV-1 particle release required AP-1 and AP-3, but not AP-5. AP-2, an essential component of clathrin-mediated endocytosis, which was previously shown to be inhibitory to HIV-1 particle release, had no effect on HIV-2. The differential requirement for adaptor protein complexes confirmed that HIV-1 and HIV-2 Gag have distinct cellular trafficking pathways, and that HIV-2 particles may be more susceptible to degradation prior to release.

  10. CAT Guide and Beamline Directory. A key to APS Collaborative Access Teams

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-07-08

    The Advanced Photon Source (APS), a national user facility for synchrotrons radiation research, is located at Argonne National Laboratory, approximately 25 miles southwest of Chicago, Illinois. The APS is considered a third-generation synchrotrons radiation facility (specifically designed to accommodate insertion devices to serve as radiation sources) and is one of three such facilities in the world. Currently, it is the most brilliant source in the United States for research in such diverse fields as biology, medicine, materials science, chemistry, geology, agriculture and soil science, physics, and manufacturing technology. Researchers use the APS either as members of Collaborative Access Teams (CATS) or as Independent Investigators (IIs). CATS are responsible for designing, building, and operating beamlines in one or more sectors, each sector consisting of an insertion-device (ID) beamline and a bending-magnet (BM) beamline. Each beamline is designed to accommodate a specific type of research program(s) and is optimized accordingly. CAT members are entitled to use 75% of the available beam time to pursue CAT research goals. The remaining 25% of the available beam time must be made available to IIs. This document was written to help prospective IIs determine which beamlines are suitable for their specific experiments.

  11. Distinct Temporal Regulation of RET Isoform Internalization: Roles of Clathrin and AP2.

    Science.gov (United States)

    Crupi, Mathieu J F; Yoganathan, Piriya; Bone, Leslie N; Lian, Eric; Fetz, Andrew; Antonescu, Costin N; Mulligan, Lois M

    2015-11-01

    The RET receptor tyrosine kinase (RTK) contributes to kidney and nervous system development, and is implicated in a number of human cancers. RET is expressed as two protein isoforms, RET9 and RET51, with distinct interactions and signaling properties that contribute to these processes. RET isoforms are internalized from the cell surface into endosomal compartments in response to glial cell line-derived neurotropic factor (GDNF) ligand stimulation but the specific mechanisms of RET trafficking remain to be elucidated. Here, we used total internal reflection fluorescence (TIRF) microscopy to demonstrate that RET internalization occurs primarily through clathrin coated pits (CCPs). Activated RET receptors colocalize with clathrin, but not caveolin. The RET51 isoform is rapidly and robustly recruited to CCPs upon GDNF stimulation, while RET9 recruitment occurs more slowly and is less pronounced. We showed that the clathrin-associated adaptor protein complex 2 (AP2) interacts directly with each RET isoform through its AP2 μ subunit, and is important for RET internalization. Our data establish that interactions with the AP2 complex promote RET receptor internalization via clathrin-mediated endocytosis but that RET9 and RET51 have distinct internalization kinetics that may contribute to differences in their biological functions.

  12. Effect of multifunctional protein YB-1 on the AP site cleavage by AP endonuclease 1 and tyrosyl phosphodiesterase 1

    Directory of Open Access Journals (Sweden)

    Ovchinnikov L. P.

    2012-07-01

    Full Text Available Apurinic/apyrimidinic sites (AP sites which represent one of the most abundantly generated DNA lesions in the cell are generally repaired by base excision repair (BER pathway. Multifunctional protein YB-1 is known to participate in cellular response to genotoxic stress and was shown to interact with several components of BER – DNA glycosylases NTH1, NEIL2, DNA polymerase and DNA ligase III. Therefore, it is of great interest to investigate the influence of YB-1 on one of the major BER enzymes, responsible for AP site cleavage, AP endonuclease APE1, and on tyrosyl phosphodiesterase Tdp1, participating in APE1 independent pathway of AP site repair. Aim. Effect of multifunctional protein YB-1 on the AP site cleavage by the activities of APE1 and Tdp1 was studied. Methods. Gel-mobility shift assays and enzyme activity tests. Results. YB-1 was shown to inhibit the cleavage of AP site located in single-stranded DNA by both APE1 and Tdp1. Stimulation of APE1 activity on protruding double-stranded DNA in the presence of YB-1 was observed, whereas no effect on Tdp1-mediated cleavage of AP site in double-stranded DNA was found. Conclusions. YB-1 can modulate the repair of AP sites in DNA by both positively stimulating APE1 during the classic BER of AP sites and avoiding a possible generation of doublestrand breaks, arising from the cleavage of single-stranded portion of DNA substrate already used by different DNA-processing pathway

  13. Studying Plant-Rhizobium Mutualism in the Biology Classroom: Connecting the Big Ideas in Biology through Inquiry

    Science.gov (United States)

    Suwa, Tomomi; Williamson, Brad

    2014-01-01

    We present a guided-inquiry biology lesson, using the plant-rhizobium symbiosis as a model system. This system provides a rich environment for developing connections between the big ideas in biology as outlined in the College Board's new AP Biology Curriculum. Students gain experience with the practice of scientific investigation, from…

  14. Studying Plant-Rhizobium Mutualism in the Biology Classroom: Connecting the Big Ideas in Biology through Inquiry

    Science.gov (United States)

    Suwa, Tomomi; Williamson, Brad

    2014-01-01

    We present a guided-inquiry biology lesson, using the plant-rhizobium symbiosis as a model system. This system provides a rich environment for developing connections between the big ideas in biology as outlined in the College Board's new AP Biology Curriculum. Students gain experience with the practice of scientific investigation, from…

  15. The AP2/EREBP family of plant transcription factors.

    Science.gov (United States)

    Riechmann, J L; Meyerowitz, E M

    1998-06-01

    AP2 (APETALA2) and EREBPs (ethylene-responsive element binding proteins) are the prototypic members of a family of transcription factors unique to plants, whose distinguishing characteristic is that they contain the so-called AP2 DNA-binding domain. AP2/ REBP genes form a large multigene family, and they play a variety of roles throughout the plant life cycle: from being key regulators of several developmental processes, like floral organ identity determination or control of leaf epidermal cell identity, to forming part of the mechanisms used by plants to respond to various types of biotic and environmental stress. The molecular and biochemical characteristics of the AP2/EREBP transcription factors and their diverse functions are reviewed here, and this multigene family is analyzed within the context of the Arabidopsis thaliana genome sequence project.

  16. Tank characterization report for double shell tank 241-AP-104

    Energy Technology Data Exchange (ETDEWEB)

    Winkelman, W.D., Westinghouse Hanford

    1996-08-07

    This document summarizes the information on the historical uses, present status, and the sampling and analysis results of waste stored in Tank 241-AP-104. This report supports the requirements of Tri-Party Agreement Milestone M-44-09.

  17. Lumbar pedicle screw placement: Using only AP plane imaging

    Directory of Open Access Journals (Sweden)

    Anil Sethi

    2012-01-01

    Conclusion: Placement of pedicle screws under fluoroscopic guidance using AP plane imaging alone with tactile guidance is safe, fast, and reliable. However, a good understanding of the radiographic landmarks is a prerequisite.

  18. A hot-spare injector for the APS linac.

    Energy Technology Data Exchange (ETDEWEB)

    Lewellen, J. W.

    1999-04-13

    Last year a second-generation SSRL-type thermionic cathode rf gun was installed in the Advanced Photon Source (APS) linac. This gun (referred to as ''gun2'') has been successfully commissioned and now serves as the main injector for the APS linac, essentially replacing the Koontz-type DC gun. To help ensure injector availability, particularly with the advent of top-up mode operation at the APS, a second thermionic-cathode rf gun will be installed in the APS linac to act as a hot-spare beam source. The hot-spare installation includes several unique design features, including a deep-orbit Panofsky-style alpha magnet. Details of the hot-spare beamline design and projected performance are presented, along with some plans for future performance upgrades.

  19. Roles of AP-2 in clathrin-mediated endocytosis.

    Directory of Open Access Journals (Sweden)

    Emmanuel Boucrot

    Full Text Available BACKGROUND: The notion that AP-2 clathrin adaptor is an essential component of an endocytic clathrin coat appears to conflict with recent observations that substantial AP-2 depletion, using RNA interference with synthesis of AP-2 subunits, fails to block uptake of certain ligands known to internalize through a clathrin-based pathway. METHODOLOGY/PRINCIPAL FINDINGS: We report here the use of in vivo imaging data obtained by spinning-disk confocal microscopy to study the formation of clathrin-coated structures at the plasma membranes of BSC1 and HeLa cells depleted by RNAi of the clathrin adaptor, AP-2. Very few clathrin coats continue to assemble after AP-2 knockdown. Moreover, there is a total absence of clathrin-containing structures completely lacking AP-2 while all the remaining coats still contain a small amount of AP-2. These observations suggest that AP-2 is essential for endocytic coated-pit and coated-vesicle formation. We also find that AP-2 knockdown strongly inhibits light-density lipoprotein (LDL receptor-mediated endocytosis, as long as cells are maintained in complete serum and at 37 degrees C. If cells are first incubated with LDL at 4 degrees C, followed by warming, there is little or no decrease in LDL uptake with respect to control cells. LDL uptake at 37 degrees C is also not affected in AP-2 depleted cells first deprived of LDL by incubation with either serum-starved or LDL-starved cells for 24 hr. The LDL-deprived cells display a significant increase in endocytic structures enriched on deeply invaginated tubes that contain LDL and we suggest that under this condition of stress, LDL might enter through this alternative pathway. CONCLUSIONS/SIGNIFICANCE: These results suggest that AP-2 is essential for endocytic clathrin coated-pit and coated-vesicle formation. They also indicate that under normal conditions, functional endocytic clathrin coated pits are required for LDL internalization. We also show that under certain

  20. Hands-on-Entropy, Energy Balance with Biological Relevance

    Science.gov (United States)

    Reeves, Mark

    2015-03-01

    Entropy changes underlie the physics that dominates biological interactions. Indeed, introductory biology courses often begin with an exploration of the qualities of water that are important to living systems. However, one idea that is not explicitly addressed in most introductory physics or biology textbooks is important contribution of the entropy in driving fundamental biological processes towards equilibrium. From diffusion to cell-membrane formation, to electrostatic binding in protein folding, to the functioning of nerve cells, entropic effects often act to counterbalance deterministic forces such as electrostatic attraction and in so doing, allow for effective molecular signaling. A small group of biology, biophysics and computer science faculty have worked together for the past five years to develop curricular modules (based on SCALEUP pedagogy). This has enabled students to create models of stochastic and deterministic processes. Our students are first-year engineering and science students in the calculus-based physics course and they are not expected to know biology beyond the high-school level. In our class, they learn to reduce complex biological processes and structures in order model them mathematically to account for both deterministic and probabilistic processes. The students test these models in simulations and in laboratory experiments that are biologically relevant such as diffusion, ionic transport, and ligand-receptor binding. Moreover, the students confront random forces and traditional forces in problems, simulations, and in laboratory exploration throughout the year-long course as they move from traditional kinematics through thermodynamics to electrostatic interactions. This talk will present a number of these exercises, with particular focus on the hands-on experiments done by the students, and will give examples of the tangible material that our students work with throughout the two-semester sequence of their course on introductory

  1. The Dushak–Erekdag Survey of roAp Stars

    Indian Academy of Sciences (India)

    Tatyana Dorokhova; Nikolay Dorokhov

    2005-06-01

    The search of roAp stars at Mt. Dushak–Erekdag Observatory was started in 1992 using the 0.8m Odessa telescope equipped with a two-star high-speed photometer. We have observed more than a dozen stars so far and discovered HD 99563 as roAp star while BD+8087 is suspected to have rapid oscillations. Negative results of our observations for the search of rapid oscillations in four stars in NGC 752 are also discussed.

  2. GeoDCAT-AP: Use cases and open issues

    OpenAIRE

    PEREGO ANDREA; FRIIS-CHRISTENSEN Anders; Lutz, Michael

    2016-01-01

    This paper illustrates some issues and use cases identified during the design and implementation of GeoDCAT-AP, a metadata profile aiming to provide a representation of geospatial metadata compliant with the DCAT application profile for European data portals (DCAT-AP). In particular, the paper focuses on those issues that may have a possible relevance also outside the geospatial domain, covering topics concerning metadata profile-based negotiation, publishing metadata on the Web, represen...

  3. AP-102/104 Retrieval control system qualification test procedure

    Energy Technology Data Exchange (ETDEWEB)

    RIECK, C.A.

    1999-05-18

    This Qualification Test Procedure documents the results of the qualification testing that was performed on the Project W-211, ''Initial Tank Retrieval Systems,'' retrieval control system (RCS) for tanks 241-AP-102 and 241-AP-104. The results confirm that the RCS has been programmed correctly and that the two related hardware enclosures have been assembled in accordance with the design documents.

  4. AP1000模块化施工分析%Analysis of the AP1000 Modular Construction

    Institute of Scientific and Technical Information of China (English)

    邹玮

    2014-01-01

    文章针对AP1000机组模块化施工的技术和进度特点进行了分析,特别是对AP1000模块化施工面临的挑战和问题进行了深入的分析.这些挑战和问题有些是首台AP1000机组所特有的,有些将在后续AP1000机组建设过程中继续存在,文章分别阐述并提出了几项建议措施.

  5. Data of evolutionary structure change: 1AP4A-1CLMA [Confc[Archive

    Lifescience Database Archive (English)

    Full Text Available 1AP4A-1CLMA 1AP4 1CLM A A ---------------------------------------...DDS LTEEQIAEFKEAFALFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLSLMARKMKEQDSEEELIEAFKVFDR...36 1CLM A 1CLMA

  6. DNA cleavage at the AP site via β-elimination mediated by the AP site-binding ligands.

    Science.gov (United States)

    Abe, Yukiko S; Sasaki, Shigeki

    2016-02-15

    DNA is continuously damaged by endogenous and exogenous factors such as oxidation and alkylation. In the base excision repair pathway, the damaged nucleobases are removed by DNA N-glycosylase to form the abasic sites (AP sites). The alkylating antitumor agent exhibits cytotoxicity through the formation of the AP site. Therefore blockage or modulation of the AP site repair pathway may enhance the antitumor efficacy of DNA alkylating agents. In this study, we have examined the effects of the nucleobase-polyamine conjugated ligands (G-, A-, C- and T-ligands) on the cleavage of the AP site. The G- and A-ligands cleaved DNA at the AP site by promoting β-elimination in a non-selective manner by the G-ligand, and in a selective manner for the opposing dT by the A-ligand. These results suggest that the nucleobase-polyamine conjugate ligands may have the potential for enhancement of the cytotoxicities of the AP site.

  7. Biological aspects of the DM28C clone of Trypanosoma cruzi after metacylogenesis in chemically defined media Aspectos biológicos do clone Dm 28c de Trypanosoma cruzi após metaciclogênese em meio quimicamente definido

    Directory of Open Access Journals (Sweden)

    Victor T. Contreras

    1988-03-01

    Full Text Available The biological characterization of the Trypanosoma cruzi clone Dm 28c in terms of its growth in LIT medium, cell-cycle, infectivity to mice and interaction with professional and non-professional phagocytic cells shows that it behaves as a bona fide T. cruzi representant. The biological properties of this myotropic clone do not change according to the origin of the trypomastigote forms (i. e., from triatomines, infected mice, cell-culture or from the chemically defined TAUP and TAU3AAG media. In addition Dm 28c metacyclic trypomastigotes from TAU3AAG medium display a high infectivity level to fibroblasts and muscle cells. Experiments on binding of cationized ferritin to trypomastigotes surface show the existence of cap-like structures of ferritin in regions near the kinetoplast. However the nature and role of these anionic sites remain to be determined. The results indicate that metacyclic trypomastigotes from Dm 28c clone obtained under chemically defined conditions reproduce the biological behaviour of T. cruzi, rendering this system very suitable for the study of cell-parasite interactions and for the isolation of trypanosome relevant macromolecules.A caracterização biológica do clone Dm 28c de Trypanosoma cruzi em termos do seu crescimento em meio LIT, ciclo celular, infectividade para camundongos e interação com células fagocíticas profissionais e não-profissionais, mostra que o mesmo comporta-se como um fiel representante da espécie T. cruzi. As propriedades biológicas deste clone miotrópico não mudam de acordo com a proveniência das formas tripomastigotas (i. e., de triatomíneos, de camundongos infectados, de cultura celular ou dos meios quimicamente definidos TAUP e TAU3AAG. Ainda mais, formas tripomastigotas metacíclicas do clone Dm 28c derivado do meio TAU3AAG apresentam um alto grau de infectividade para fibroblastos e células de músculo. Experimentos de ligação de ferritina cationizada à superfície de

  8. An AP4B1 frameshift mutation in siblings with intellectual disability and spastic tetraplegia further delineates the AP-4 deficiency syndrome.

    Science.gov (United States)

    Abdollahpour, Hengameh; Alawi, Malik; Kortüm, Fanny; Beckstette, Michael; Seemanova, Eva; Komárek, Vladimír; Rosenberger, Georg; Kutsche, Kerstin

    2015-02-01

    The recently proposed adaptor protein 4 (AP-4) deficiency syndrome comprises a group of congenital neurological disorders characterized by severe intellectual disability (ID), delayed or absent speech, hereditary spastic paraplegia, and growth retardation. AP-4 is a heterotetrameric protein complex with important functions in vesicle trafficking. Mutations in genes affecting different subunits of AP-4, including AP4B1, AP4E1, AP4S1, and AP4M1, have been reported in patients with the AP-4 deficiency phenotype. We describe two siblings from a non-consanguineous couple who presented with severe ID, absent speech, microcephaly, growth retardation, and progressive spastic tetraplegia. Whole-exome sequencing in the two patients identified the novel homozygous 2-bp deletion c.1160_1161delCA (p.(Thr387Argfs*30)) in AP4B1. Sanger sequencing confirmed the mutation in the siblings and revealed it in the heterozygous state in both parents. The AP4B1-associated phenotype has previously been assigned to spastic paraplegia-47. Identification of a novel AP4B1 alteration in two patients with clinical manifestations highly similar to other individuals with mutations affecting one of the four AP-4 subunits further supports the observation that loss of AP-4 assembly or functionality underlies the common clinical features in these patients and underscores the existence of the clinically recognizable AP-4 deficiency syndrome.

  9. Robust dynamic balance of AP-1 transcription factors in a neuronal gene regulatory network

    Directory of Open Access Journals (Sweden)

    Schwaber James S

    2010-12-01

    Full Text Available Abstract Background The octapeptide Angiotensin II is a key hormone that acts via its receptor AT1R in the brainstem to modulate the blood pressure control circuits and thus plays a central role in the cardiac and respiratory homeostasis. This modulation occurs via activation of a complex network of signaling proteins and transcription factors, leading to changes in levels of key genes and proteins. AT1R initiated activity in the nucleus tractus solitarius (NTS, which regulates blood pressure, has been the subject of extensive molecular analysis. But the adaptive network interactions in the NTS response to AT1R, plausibly related to the development of hypertension, are not understood. Results We developed and analyzed a mathematical model of AT1R-activated signaling kinases and a downstream gene regulatory network, with structural basis in our transcriptomic data analysis and literature. To our knowledge, our report presents the first computational model of this key regulatory network. Our simulations and analysis reveal a dynamic balance among distinct dimers of the AP-1 family of transcription factors. We investigated the robustness of this behavior to simultaneous perturbations in the network parameters using a novel multivariate approach that integrates global sensitivity analysis with decision-tree methods. Our analysis implicates a subset of Fos and Jun dependent mechanisms, with dynamic sensitivities shifting from Fos-regulating kinase (FRK-mediated processes to those downstream of c-Jun N-terminal kinase (JNK. Decision-tree analysis indicated that while there may be a large combinatorial functional space feasible for neuronal states and parameters, the network behavior is constrained to a small set of AP-1 response profiles. Many of the paths through the combinatorial parameter space lead to a dynamic balance of AP-1 dimer forms, yielding a robust AP-1 response counteracting the biological variability. Conclusions Based on the simulation

  10. Stochastic Methods in Biology

    CERN Document Server

    Kallianpur, Gopinath; Hida, Takeyuki

    1987-01-01

    The use of probabilistic methods in the biological sciences has been so well established by now that mathematical biology is regarded by many as a distinct dis­ cipline with its own repertoire of techniques. The purpose of the Workshop on sto­ chastic methods in biology held at Nagoya University during the week of July 8-12, 1985, was to enable biologists and probabilists from Japan and the U. S. to discuss the latest developments in their respective fields and to exchange ideas on the ap­ plicability of the more recent developments in stochastic process theory to problems in biology. Eighteen papers were presented at the Workshop and have been grouped under the following headings: I. Population genetics (five papers) II. Measure valued diffusion processes related to population genetics (three papers) III. Neurophysiology (two papers) IV. Fluctuation in living cells (two papers) V. Mathematical methods related to other problems in biology, epidemiology, population dynamics, etc. (six papers) An important f...

  11. APS-U LATTICE DESIGN FOR OFF-AXIS ACCUMULATION

    Energy Technology Data Exchange (ETDEWEB)

    Sun, Yipeng; Borland, M.; Lindberg, R.; Sajaev, V.

    2017-06-25

    A 67-pm hybrid-seven-bend achromat (H7BA) lattice is being proposed for a future Advanced Photon Source (APS) multi-bend-achromat (MBA) upgrade project. This lattice design pushes for smaller emittance and requires use of a swap-out (on-axis) injection scheme due to limited dynamic acceptance. Alternate lattice design work has also been performed for the APS upgrade to achieve better beam dynamics performance than the nominal APS MBA lattice, in order to allow off-axis accumulation. Two such alternate H7BA lattice designs, which target a still-low emittance of 90 pm, are discussed in detail in this paper. Although the single-particle-dynamics performance is good, simulations of collective effects indicate that surprising difficulty would be expected accumulating high single-bunch charge in this lattice. The brightness of the 90-pm lattice is also a factor of two lower than the 67-pm H7BA lattice.

  12. Westinghouse plans global new builds for AP1000

    Energy Technology Data Exchange (ETDEWEB)

    Mitev, Lubomir [NucNet, Brussels (Belgium)

    2014-10-15

    Interview with Danny Roderick, Westinghouse Electric Company, President and Chief Executive Officer since September 2012, about perspectives and future plans for AP1000 new build worldwide. Within three to four years there wille be 'shovels in the ground' for three new AP1000 reactors in the UK, as well as new units in China and Bulgaria. Four AP1000 reactors are under construction in the United States at Vogtle and VC Summer, and soon at Turkey Point. Additionally Danny Roderick spoke about the acquisition of NuGen, technology transfer, the influence of the Ukraine crises on the nuclear market in East Europe and the future need for more nuclear worldwide and in the UK and Bulgaria.

  13. Prevention of aspiration pneumonia (AP) with oral care.

    Science.gov (United States)

    Tada, Akio; Miura, Hiroko

    2012-01-01

    AP is a major cause of morbidity and mortality in elderly patients, especially frail elderly patients. The aim of this article is to review effect of oral care, including oral hygiene and improvement of oral function, on the prevention of AP among elderly people in hospitals and nursing homes. There is now a substantial body of work studying the effect of oral care on the prevention of respiratory diseases. Oral hygiene, consisting of oral decontamination and mechanical cleaning by dental professionals, has resulted in significant clinical effects (decreased incidence of pneumonia and decreased mortality from respiratory diseases) in clinical randomized trials. Moreover, studies examining oral colonization by pneumonia pathogens have shown the effect of oral hygiene on eliminating these pathogens. In addition, swallowing training has been shown to improve the movement and function of swallowing-related muscles, also resulting in decreased incidence of pneumonia. These findings support the contention that oral care is effective in the prevention of AP.

  14. Past, Present, and Future of AP Chemistry: A Brief History of Course and Exam Alignment Efforts

    Science.gov (United States)

    Magrogan, Serena

    2014-01-01

    As part of the Advanced Placement (AP) Program's commitment to continually enhance alignment with current best practices in college-level learning, the AP Program is currently evaluating and redesigning courses and exams, one of which launched during the 2013-2014 academic school year: AP chemistry. The history of the AP chemistry course and…

  15. Tromboembolia pulmonar após videoartroscopia de ombro

    OpenAIRE

    Fabio Farina Dal Molin; Siluê Franzoni Dal Molin

    2010-01-01

    Embora fenômenos tromboembólicos sejam complicações frequentes em cirurgias dos membros inferiores, apenas dois relatos de casos de tromboembolia pulmonar após artroscopia de ombro são encontrados na literatura. É descrito o caso de uma paciente com 76 anos com embolia pulmonar bilateral após artroscopia cirúrgica do ombro. Não foram encontradas anormalidades vasculares e nenhuma origem do trombo foi detectada, ficando desconhecida a causa exata responsável pela tromboembolia.Though thromboem...

  16. THE PARALLEL RECURSIVE AP ADAPTIVE ALGORITHM BASED ON VOLTERRA SERIES

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    Aiming at the nonlinear system identification problem, a parallel recursive affine projection (AP) adaptive algorithm for the nonlinear system based on Volterra series is presented in this paper. The algorithm identifies in parallel the Volterra kernel of each order, recursively estimate the inverse of the autocorrelation matrix for the Volterra input of each order, and remarkably improve the convergence speed of the identification process compared with the NLMS and conventional AP adaptive algorithm based on Volterra series. Simulation results indicate that the proposed method in this paper is efficient.

  17. First Magnetic Doppler Images of a roAp star

    Science.gov (United States)

    Lüftinger, T.; Kochukhov, O.; Ryabchikova, T.; Weiss, W. W.; Ilyin, I.

    2007-06-01

    We present the first analysis of the magnetic field geometry and elemental abundance distributions on the surface of a rapidly oscillating Ap (roAp) star, using an elaborate magnetic Doppler Imaging (MDI) code (Piskunov et al. 2002, Kochukhov et al. 2002), inv, which allows to reconstruct simultaneously and consistently the magnetic field geometry and abundance distributions on a stellar surface without any a priori assumptions. We analysed Stokes I and V time series obtained with the SOFIN polarimeter and recovered the magnetic field and surface abundance structures of Fe and Nd (among others). These two elements are found to be anticorrelated.

  18. ION EFFECTS IN THE APS PARTICLE ACCUMULATOR RING

    Energy Technology Data Exchange (ETDEWEB)

    Calvey, J.; Harkay, K.; Yao, CY.

    2017-06-25

    Trapped ions in the APS Particle Accumulator Ring (PAR) lead to a positive coherent tune shift in both planes, which increases along the PAR cycle as more ions accumulate. This effect has been studied using an ion simulation code developed at SLAC. After modifying the code to include a realistic vacuum profile, multiple ionization, and the effect of shaking the beam to measure the tune, the simulation agrees well with our measurements. This code has also been used to evaluate the possibility of ion instabilities at the high bunch charge needed for the APS-Upgrade.

  19. Greenhouse evaluation of Bacillus subtilis AP-01 and Trichoderma harzianum AP-001 in controlling tobacco diseases Avaliação em estufa de dois agentes biológicos de controle de doenças do tabaco

    Directory of Open Access Journals (Sweden)

    Monchan Maketon

    2008-06-01

    Full Text Available Two biological control agents, Bacillus subtilis AP-01 (LarminarTM and Trichoderma harzianum AP-001 (TrisanTM alone or/in combination were investigated in controlling three tobacco diseases, including bacterial wilt (Ralstonia solanacearum, damping-off (Pythium aphanidermatum, and frogeye leaf spot (Cercospora nicotiana. Tests were performed in greenhouse by soil sterilization prior to inoculation of the pathogens. Bacterial-wilt and damping off pathogens were drenched first and followed with the biological control agents and for comparison purposes, two chemical fungicides. But for frogeye leaf spot, which is an airborne fungus, a spraying procedure for every treatment including a chemical fungicide was applied instead of drenching. Results showed that neither B. subtilis AP-01 nor T. harzianum AP-001 alone could control the bacterial wilt, but when combined, their controlling capabilities were as effective as a chemical treatment. These results were also similar for damping-off disease when used in combination. In addition, the combined B. subtilis AP-01 and T. harzianum AP-001 resulted in a good frogeye leaf spot control, which was not significantly different from the chemical treatment.Dois agentes de controle biológico, Bacillus subtilis AP-01 (Larminar® e Trichoderma harzianum AP-001 (Trisan® foram avaliados separadamente ou em combinação quanto à capacidade de controlar três doenças do tabaco: murcha bacteriana (bacterial wilt, Ralstonia solanacearum, tombamento de mudas (damping-off, Pythium aphanidermatum, e mancha olho-de-rã (frogeye leaf spot, Cercospora nicotiana. Os testes foram realizados em estufa, esterilizando-se o solo antes da inoculação dos patógenos. Os patógenos causadores da murcha bacteriana e tombamento de mudas foram inicialmente encharcados e acompanhados com os agentes de controle biológico e, para comparação, com um fungicida químico. Para a mancha olho-de-rã, causada por um fungo anem

  20. Berberine inhibits cyclin D1 expression via suppressed binding of AP-1 transcription factors to CCND1 AP-1 motif

    Institute of Scientific and Technical Information of China (English)

    Ye LUO; Yu HAO; Tai-ping SHI; Wei-wei DENG; Na LI

    2008-01-01

    Aim: To verify the suppressive effect of berberine on the proliferation of the human pulmonary giant cell carcinoma cell line PG and to demonstrate the mecha-nisms behind the antitumoral effects of berberine. Methods: The proliferative effects of PG cells were detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide colorimetry. The cell cycle was examined by flow cytometry. The expression level of cyclin D1 was detected by RT-PCR. The activities of the activating protein-1 (AP-1) and NF-κB signaling pathways related to cyclin D1 were examined by luciferase assay. The cytoplasmic level of c-Jun was detected by Western blot analysis. An electrophoretic mobility shift assay was used to examiae the binding of transcription factors to the cyclin D1 gene (CCNDl) AP-1 motif. Results: The results showed that the proliferation of PG cells treated with different concentrations (10, 20, and 40 μg/mL) of berberine for 24 and 48 h was suppressed significantly compared to the control group. After treatment with berberine, the proportion of PG cells at the G0/G1 phase increased, while cells at the S and G2/M phases decreased. Berberine could inhibit the expression of cyclin D1 in PG cells. Berberine inhibited the activity of the AP-1 signaling pathway, but had no significant effect on the NF-κB signaling pathway. Berberine suppressed the expression of c-Jun and decreased the binding of tran-scription factors to the CCND1 AP-1 motif. Conclusion: Berberine suppresses the activity of the AP-1 signaling pathway and decreases the binding of transcrip-tion factors to the CCND1 AP-1 motif. This is one of the important mechanisms behind the antitumoral effects of berberine as a regulator of cyclin D1.

  1. All biology is computational biology.

    Science.gov (United States)

    Markowetz, Florian

    2017-03-01

    Here, I argue that computational thinking and techniques are so central to the quest of understanding life that today all biology is computational biology. Computational biology brings order into our understanding of life, it makes biological concepts rigorous and testable, and it provides a reference map that holds together individual insights. The next modern synthesis in biology will be driven by mathematical, statistical, and computational methods being absorbed into mainstream biological training, turning biology into a quantitative science.

  2. All biology is computational biology

    Science.gov (United States)

    2017-01-01

    Here, I argue that computational thinking and techniques are so central to the quest of understanding life that today all biology is computational biology. Computational biology brings order into our understanding of life, it makes biological concepts rigorous and testable, and it provides a reference map that holds together individual insights. The next modern synthesis in biology will be driven by mathematical, statistical, and computational methods being absorbed into mainstream biological training, turning biology into a quantitative science. PMID:28278152

  3. SEPTUM MAGNET DESIGN FOR THE APS-U

    Energy Technology Data Exchange (ETDEWEB)

    Abliz, M.; Jaski, M.; Xiao, A.; Wienands, U.; Cease, H.; Borland, M.; Decker, G.; Kerby, J.

    2017-06-25

    The Advanced Photon Source is in the process of upgrading its storage ring from a double-bend to a multi-bend lattice as part of the APS Upgrade Project (APS-U). A swap-out injection scheme is planned for the APS-U to keep a constant beam current and to enable a small dynamic aperture. A septum magnet with a minimum thickness of 2 mm and an injection field of 1.06 T has been designed, delivering the required total deflecting angle is 89 mrad with a ring energy of 6 GeV. The stored beam chamber has an 8 mm x 6 mm super-ellipsoidal aperture. The magnet is straight; however, it is tilted in yaw, roll, and pitch from the stored beam chamber to meet the on axis swap out injection requirements for the APS-U lattice. In order to minimize the leakage field inside the stored beam chamber, four different techniques were utilized in the design. As a result, the horizontal deflecting angle of the stored beam was held to only 5 µrad, and the integrated skew quadrupole inside the stored beam chamber was held to 0.09 T. The detailed techniques that were applied to the design, field multipoles, and resulting trajectories of the injected and stored beams are reported.

  4. Integrating Particulate Representations into AP Chemistry and Introductory Chemistry Courses

    Science.gov (United States)

    Prilliman, Stephen G.

    2014-01-01

    The College Board's recently revised curriculum for advanced placement (AP) chemistry places a strong emphasis on conceptual understanding, including representations of particle phenomena. This change in emphasis is informed by years of research showing that students could perform algorithmic calculations but not explain those calculations…

  5. Army and Marine Corps Active Protection System (APS) Efforts

    Science.gov (United States)

    2016-08-30

    Eastern Europe ....................... 4 Basic APS Considerations and Theory ... Information .......................................................................................................... 27 Army and Marine Corps Active...steel armor, which detonates when struck by a projectile in order to disrupt the armor- piercing jet produced by RPG, ATGM, and main gun-shaped

  6. Research on CoAP%CoAP协议研究

    Institute of Scientific and Technical Information of China (English)

    刘紫青; 程燕; 关联; 桑梓勤; 郝俊瑞

    2013-01-01

    For some M2M applications, there exists problems of constrained networks and nodes, and the HTTP isn't applicable. The Constrained Application Protocol (CoAP) is described and analyzed in this paper, and the mapping between CoAP and HTTP is also included. Then, the comparison of the two protocols is researched. Finally, it can be concluded that CoAP is simple and its overhead is very low, which can well meet the application layer' s communications requirements in the constrained environment.%针对某些M2M应用中存在网络和节点资源受限的情况,不适用HTTP,介绍和分析了受限应用协议CoAP(Constrained Application Protocol)的特点及其与HTTP的映射,并将CoAP和HTTP进行对比研究,发现CoAP简单、开销小,能很好地满足资源受限环境的应用层通信需求.

  7. Integrating Particulate Representations into AP Chemistry and Introductory Chemistry Courses

    Science.gov (United States)

    Prilliman, Stephen G.

    2014-01-01

    The College Board's recently revised curriculum for advanced placement (AP) chemistry places a strong emphasis on conceptual understanding, including representations of particle phenomena. This change in emphasis is informed by years of research showing that students could perform algorithmic calculations but not explain those calculations…

  8. Tead and AP1 Coordinate Transcription and Motility.

    Science.gov (United States)

    Liu, Xiangfan; Li, Huapeng; Rajurkar, Mihir; Li, Qi; Cotton, Jennifer L; Ou, Jianhong; Zhu, Lihua J; Goel, Hira L; Mercurio, Arthur M; Park, Joo-Seop; Davis, Roger J; Mao, Junhao

    2016-02-09

    The Tead family transcription factors are the major intracellular mediators of the Hippo-Yap pathway. Despite the importance of Hippo signaling in tumorigenesis, Tead-dependent downstream oncogenic programs and target genes in cancer cells remain poorly understood. Here, we characterize Tead4-mediated transcriptional networks in a diverse range of cancer cells, including neuroblastoma, colorectal, lung, and endometrial carcinomas. By intersecting genome-wide chromatin occupancy analyses of Tead4, JunD, and Fra1/2, we find that Tead4 cooperates with AP1 transcription factors to coordinate target gene transcription. We find that Tead-AP1 interaction is JNK independent but engages the SRC1-3 co-activators to promote downstream transcription. Furthermore, we show that Tead-AP1 cooperation regulates the activity of the Dock-Rac/CDC42 module and drives the expression of a unique core set of target genes, thereby directing cell migration and invasion. Together, our data unveil a critical regulatory mechanism underlying Tead- and AP1-controlled transcriptional and functional outputs in cancer cells. Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.

  9. Tead and AP1 Coordinate Transcription and Motility

    Directory of Open Access Journals (Sweden)

    Xiangfan Liu

    2016-02-01

    Full Text Available The Tead family transcription factors are the major intracellular mediators of the Hippo-Yap pathway. Despite the importance of Hippo signaling in tumorigenesis, Tead-dependent downstream oncogenic programs and target genes in cancer cells remain poorly understood. Here, we characterize Tead4-mediated transcriptional networks in a diverse range of cancer cells, including neuroblastoma, colorectal, lung, and endometrial carcinomas. By intersecting genome-wide chromatin occupancy analyses of Tead4, JunD, and Fra1/2, we find that Tead4 cooperates with AP1 transcription factors to coordinate target gene transcription. We find that Tead-AP1 interaction is JNK independent but engages the SRC1–3 co-activators to promote downstream transcription. Furthermore, we show that Tead-AP1 cooperation regulates the activity of the Dock-Rac/CDC42 module and drives the expression of a unique core set of target genes, thereby directing cell migration and invasion. Together, our data unveil a critical regulatory mechanism underlying Tead- and AP1-controlled transcriptional and functional outputs in cancer cells.

  10. Penetration dynamics of AP8 in thin ceramic tiles

    NARCIS (Netherlands)

    Abadjieva, E.; Khoe, Y.S.

    2013-01-01

    The interaction of thin ceramic tiles with AP8 (WC core, 7,62 mm) at 1000 m/s velocity has been studied experimentally and numerically. “Thin” ceramic tiles refers here to ratio of the tile thickness (t) to the projectile diameter, (d), t/d@ 1, as they are both in the same order. The method applied

  11. Cinnamon extract induces tumor cell death through inhibition of NFκB and AP1

    Directory of Open Access Journals (Sweden)

    Lee Sung

    2010-07-01

    Full Text Available Abstract Background Cinnamomum cassia bark is the outer skin of an evergreen tall tree belonging to the family Lauraceae containing several active components such as essential oils (cinnamic aldehyde and cinnamyl aldehyde, tannin, mucus and carbohydrate. They have various biological functions including anti-oxidant, anti-microbial, anti-inflammation, anti-diabetic and anti-tumor activity. Previously, we have reported that anti-cancer effect of cinnamon extracts is associated with modulation of angiogenesis and effector function of CD8+ T cells. In this study, we further identified that anti-tumor effect of cinnamon extracts is also link with enhanced pro-apoptotic activity by inhibiting the activities NFκB and AP1 in mouse melanoma model. Methods Water soluble cinnamon extract was obtained and quality of cinnamon extract was evaluated by HPLC (High Performance Liquid Chromatography analysis. In this study, we tested anti-tumor activity and elucidated action mechanism of cinnamon extract using various types of tumor cell lines including lymphoma, melanoma, cervix cancer and colorectal cancer in vitro and in vivo mouse melanoma model. Results Cinnamon extract strongly inhibited tumor cell proliferation in vitro and induced active cell death of tumor cells by up-regulating pro-apoptotic molecules while inhibiting NFκB and AP1 activity and their target genes such as Bcl-2, BcL-xL and survivin. Oral administration of cinnamon extract in melanoma transplantation model significantly inhibited tumor growth with the same mechanism of action observed in vitro. Conclusion Our study suggests that anti-tumor effect of cinnamon extracts is directly linked with enhanced pro-apoptotic activity and inhibition of NFκB and AP1 activities and their target genes in vitro and in vivo mouse melanoma model. Hence, further elucidation of active components of cinnamon extract could lead to development of potent anti-tumor agent or complementary and alternative

  12. Orchestrating A/P and D/V guidance - A Wnt/Netrin tale.

    Science.gov (United States)

    Levy-Strumpf, Naomi

    2016-01-01

    While ample information was gathered in identifying guidance cues and their downstream mediators, very little is known about how the information from multiple extracellular cues is intracellularly to generate normal patterning. Netrin and Wnt signaling pathways play key roles in normal development as well as in malignancies. In C. elegans, as in vertebrates, dorso-ventral (D/V) graded distributions of UNC-6/Netrin and antero-posterior (A/P) graded distributions of Wnts provide instructive polarity information to guide cells and axons along their respective gradients. In this commentary, I will discuss recent findings demonstrating that these 2 signaling pathways also function redundantly to regulate polarity orthogonal to the axis of their gradation. Thus, Wnt signaling components contribute to D/V polarity, while Netrin signaling components contribute to A/P polarity and their joint action collaboratively governs migratory transitions from one axis to the other. These findings pave the way to unraveling broader roles of Wnt and Netrin signaling pathways, roles that are masked due to their redundant nature, and provide a conceptually novel view of how antero-posterior and dorso-ventral guidance mechanisms are orchestrated to establish polarity in multiple biological processes.

  13. Chloroquine inhibits human CD4+ T-cell activation by AP-1 signaling modulation

    Science.gov (United States)

    Schmidt, Ralf L. J.; Jutz, Sabrina; Goldhahn, Katrin; Witzeneder, Nadine; Gerner, Marlene C.; Trapin, Doris; Greiner, Georg; Hoermann, Gregor; Steiner, Guenter; Pickl, Winfried F.; Burgmann, Heinz; Steinberger, Peter; Ratzinger, Franz; Schmetterer, Klaus G.

    2017-01-01

    Chloroquine (CQ) is widely used as an anti-inflammatory therapeutic for rheumatic diseases. Although its modes of action on the innate immune system are well described, there is still insufficient knowledge about its direct effects on the adaptive immune system. Thus, we evaluated the influence of CQ on activation parameters of human CD4+ T-cells. CQ directly suppressed proliferation, metabolic activity and cytokine secretion of T-cells following anti-CD3/anti-CD28 activation. In contrast, CQ showed no effect on up-regulation of T-cell activation markers. CQ inhibited activation of all T helper cell subsets, although IL-4 and IL-13 secretion by Th2 cells were less influenced compared to other Th-specific cytokines. Up to 10 μM, CQ did not reduce cell viability, suggesting specific suppressive effects on T-cells. These properties of CQ were fully reversible in re-stimulation experiments. Analyses of intracellular signaling showed that CQ specifically inhibited autophagic flux and additionally activation of AP-1 by reducing phosphorylation of c-JUN. This effect was mediated by inhibition of JNK catalytic activity. In summary, we characterized selective and reversible immunomodulatory effects of CQ on human CD4+ T-cells. These findings provide new insights into the biological actions of JNK/AP-1 signaling in T-cells and may help to expand the therapeutic spectrum of CQ. PMID:28169350

  14. Aviation Safety Program: Weather Accident Prevention (WxAP) Development of WxAP System Architecture And Concepts of Operation

    Science.gov (United States)

    Grantier, David

    2003-01-01

    This paper presents viewgraphs on the development of the Weather Accident Prevention (WxAP) System architecture and Concept of Operation (CONOPS) activities. The topics include: 1) Background Information on System Architecture/CONOPS Activity; 2) Activity Work in Progress; and 3) Anticipated By-Products.

  15. Heterologous expression of ApGSMT2 and ApDMT2 genes from Aphanothece halophytica enhanced drought tolerance in transgenic tobacco.

    Science.gov (United States)

    He, Ying; He, Chunmei; Li, Lihua; Liu, Zhili; Yang, Aifang; Zhang, Juren

    2011-01-01

    The glycine-methylation biosynthetic pathway of glycinebetaine (GB) has been investigated, but only a few studies on GB accumulation in transgenic higher plants have utilized this pathway. In this study, two methyltransferase genes named ApGSMT2 and ApDMT2, encoding proteins catalyzing GB biosynthesis from glycine, were cloned from a relative strain of Aphanothece halophytica. The potential roles of ApGSMT2 and ApDMT2 in GB synthesis were first examined in transgenic Escherichia coli, which had increased levels of GB and improved salt tolerance. Then ApGSMT2 and ApDMT2 were transferred into tobacco. Compared with transgenic tobacco expressing betA, transgenic tobacco co-expressing ApGSMT2 and ApDMT2 accumulated more GB and exhibited enhanced drought resistance with better germination performance, higher relative water content, less cell membrane damage and better photosynthetic capacity under drought stress. We concluded that the ApGSMT2 and ApDMT2 genes cloned in this study will be very useful for engineering GB-accumulating transgenic plants with enhanced drought resistance.

  16. Essay: Computers in the APS Editorial Office: The Early Years

    Science.gov (United States)

    Adams, Peter

    2009-03-01

    APS journals in the late 1960s began to move from hot-metal print shops to tailor-made typewriters, next to special keyboards and a small computer, then to completely photocomposed journal pages. At the same time, internal editorial operations were being converted from paper handling and data records to completely electronic procedures. By 1990, it became feasible for authors to submit manuscripts to the journal electronically.

  17. Generating Lookup Tables from the AE9/AP9 Models

    Science.gov (United States)

    2015-06-16

    NUMBER 11 5e. TASK NUMBER na 5f. WORK UNIT NUMBER na 7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) The Aerospace Corporation 2310 E. El...electrons using SOAP and a static 3D table containing data generated from the AE9/AP9 model. ........................................................ 2...used to construct a 3D data table that could be read by the Satellite Orbit Analysis Program ( SOAP ) [2], which is a program developed and maintained

  18. Characteristics of shigellosis outbreaks in the AP of Vojvodina

    OpenAIRE

    2009-01-01

    Introduction. Shigellosis causes around 165 million infections and around 1 million deaths in the world every year. Two thirds of both infections and deaths are among children younger than 10. Shigellosis mainly spreads by direct or indirect contact, but water- and food-borne outbreaks are not rare. Material and methods. A descriptive epidemiological method was used to analyse characteristics of shigellosis outbreaks in the AP of Vojvodina in the period 1979-2005. Results. During this period ...

  19. Diffusion and Settling in Ap/Bp Stars

    CERN Document Server

    Turcotte, S

    2003-01-01

    Ap/Bp stars are magnetic chemically peculiar early A and late B type stars of the main sequence. They exhibit peculiar surface abundance anomalies that are thought to be the result of gravitational settling and radiative levitation. The physics of diffusion in these stars are reviewed briefly and some model predictions are discussed. While models reproduce some observations reasonably well, more work is needed before the behavior of diffusing elements in a complex magnetic field is fully understood.

  20. On the progenitor of the Type Ic supernova 2002ap

    NARCIS (Netherlands)

    Smartt, SJ; Ramirez-Ruiz, E; Gilmore, GF; Meikle, WPS; Ferguson, AMN; Knapen, JH

    2002-01-01

    This Letter presents wide-field optical and near-IR (UBVRIHalphaK') images of the galaxy M74 that were taken between 0.6 and 8.3 yr before the discovery of the Type Ic supernova 2002ap. We have located the position of the supernova on these images with an accuracy of 0."3. We find no sign of a proge

  1. A Low-Power CoAP for Contiki

    OpenAIRE

    Kovatsch M.; Duquennoy S.; Dunkels A.

    2011-01-01

    Internet of Things devices will by and large be battery-operated, but existing application protocols have typically not been designed with power-efficiency in mind. In low-power wireless systems, power-efficiency is determined by the ability to maintain a low radio duty cycle: keeping the radio off as much as possible. We present an implementation of the IETF Constrained Application Protocol (CoAP) for the Contiki operating system that leverages the ContikiMAC low-powe...

  2. Diffusion and Settling in Ap/Bp Stars

    Energy Technology Data Exchange (ETDEWEB)

    Turcotte, S

    2003-04-09

    Ap/Bp stars are magnetic chemically peculiar early A and late B type stars of the main sequence. They exhibit peculiar surface abundance anomalies that are thought to be the result of gravitational settling and radiative levitation. The physics of diffusion in these stars are reviewed briefly and some model predictions are discussed. While models reproduce some observations reasonably well, more work is needed before the behavior of diffusing elements in a complex magnetic field is fully understood.

  3. Thermal analysis of the beam missteering in APS storage ring

    Energy Technology Data Exchange (ETDEWEB)

    Sheng, I.C.; Howell, J.; Sharma, S.

    1993-08-01

    Several bending magnet beam missteering cases have been investigated for the 7-GeV storage ring of the Advanced Photon Source (APS). One of the critical missteering events is presented in this paper. Finite element analyses are performed to solve for both temperature and stress fields. Thermally induced deflections are determined by using beam bending theory. A safe current limit is established for the storage ring chambers.

  4. BPM STABILTIY STUDIES FOR THE APS MBA UPGRADE

    Energy Technology Data Exchange (ETDEWEB)

    Lill, R.; Sereno, N.; Yang, B.

    2017-03-25

    The Advanced Photon Source (APS) is currently in the preliminary design phase for the multi-bend achromat (MBA) lattice upgrade. Beam stability is critical for the MBA and will require long term drift defined as beam mo-tion over a seven-day timescale to be no more than 1 mi-cron at the insertion device locations and beam angle change no more than 0.25 micro-radian. Mechanical stabil-ity of beam position monitor (BPM) pickup electrodes mounted on insertion device vacuum chambers place a fun-damental limitation on long-term beam stability for inser-tion device beamlines. We present the design and imple-mentation of prototype mechanical motion system (MMS) instrumentation for quantifying this type of motion specif-ically in the APS accelerator tunnel and experiment hall floor under normal operating conditions. The MMS pres-ently provides critical position information on the vacuum chamber and BPM support systems. Initial results of the R&D prototype systems have demonstrated that the cham-ber movements far exceed the long-term drift tolerance specified for the APS Upgrade MBA storage ring.

  5. AP Theory IV: Intrinsic Topological Quantum Langlands Theory

    CERN Document Server

    Winkelnkemper, H E

    2010-01-01

    Without using any moduli, sheaves, stacks, nor any analytic, nor category-type arguments, we exhibit an analogue to Geometric Langlands Theory in an entirely model-independent, non-perturbative,purely smooth topological context in Artin Presentation Theory. A basic initial feature is that AP Theory, as a whole, is already, ab initio, a universal canonical 2D sigma-model, targeting smooth, compact, simply-connected 4-manifolds with a connected boundary, and its topological Planckian quantum starting point, as well as its cone-like, infinitely-generated at each stage, graded group of homology-preserving, but topology-changing transitions/interactions, exhibit the most general qualitative S-duality. We first point out the numerous mathematically rigorous, model-free, (i.e., intrinsic), topological AP analogues with the heuristic Kapustin-Witten version of Geometric Langlands theory, as well as the crucial differences between the two theories. The latter have to exist since AP Theory deals, a priori, essentially ...

  6. The Adolescent Novel in AP English: A Response to Patricia Spencer.

    Science.gov (United States)

    McGee, Tim

    1992-01-01

    Discusses the absence of adolescent literature in Advanced Placement (AP) English classes. Describes how a class of AP students and their teacher benefited from reading Robert Cormier's "The Chocolate War." (PRA)

  7. Recombinant thermostable AP exonuclease from Thermoanaerobacter tengcongensis: cloning, expression, purification, properties and PCR application

    DEFF Research Database (Denmark)

    Dabrowski, Slawomir; Brillowska-Dabrowska, Anna; Ahring, Birgitte Kiær

    2013-01-01

    Apurinic/apyrimidinic (AP) sites in DNA are considered to be highly mutagenic and must be corrected to preserve genetic integrity, especially at high temperatures. The gene encoding a homologue of AP exonuclease was cloned from the thermophilic anaerobic bacterium Thermoanaerobacter tengcongensis...... (14000 kU) of pure His6-tagged Tte AP (153 kU/mg) from 1 liter of culture. The optimal conditions of Tte AP endo-, exonuclease and 3'-nuclease activity were investigated using fluorescein labeled dsDNA with inserted AP sites and ssDNA. Optimal Tte AP endonuclease activity was observed at 70-75 degrees C...... DNA templates. Tte AP exonuclease eliminated AP-sites in DNA template and improved the efficiency of DNA amplification....

  8. Biological computation

    CERN Document Server

    Lamm, Ehud

    2011-01-01

    Introduction and Biological BackgroundBiological ComputationThe Influence of Biology on Mathematics-Historical ExamplesBiological IntroductionModels and Simulations Cellular Automata Biological BackgroundThe Game of Life General Definition of Cellular Automata One-Dimensional AutomataExamples of Cellular AutomataComparison with a Continuous Mathematical Model Computational UniversalitySelf-Replication Pseudo Code Evolutionary ComputationEvolutionary Biology and Evolutionary ComputationGenetic AlgorithmsExample ApplicationsAnalysis of the Behavior of Genetic AlgorithmsLamarckian Evolution Genet

  9. NRC confirmatory AP600 safety system phase I testing in the ROSA/AP600 test facility

    Energy Technology Data Exchange (ETDEWEB)

    Rhee, G.S.; Kukita, Yutaka; Schultz, R.R.

    1996-03-01

    The NRC confirmatory phase I testing for the AP600 safety systems has been completed in the modified ROSA (Rig of Safety Assessment) test facility located at the Japan Atomic Energy Research Institute (JAERI) campus in Tokai, Japan. The test matrix included a variety of accident scenarios covering both design and beyond-design basis accidents. The test results indicate the AP600 safety systems as reflected in ROSA appear to perform as designed and there is no danger of core heatup for the accident scenarios investigated. In addition, no detrimental system interactions nor adverse effects of non-safety systems on the safety system functions were identified. However, three phenomena of interest have been identified for further examination to determine whether they are relevant to the AP600 plant. Those three phenomena are: (1) a potential for water hammer caused by rapid condensation which may occur following the actuation of the automatic depressurization system (ADS), (2) a large thermal gradient in the cold leg pipe where cooled water returns from the passive residual heat removal system and forms a thermally stratified layer, and (3) system-wide oscillations initiating following the ADS stage 4 actuation and persisting until the liquid in the pressurizer drains and steam generation in the core becomes insignificant.

  10. FRANX. Application for analysis and quantification of the APS fire; FRANK. Aplicacion para el analisis y cuantificacion de los APS de incendios

    Energy Technology Data Exchange (ETDEWEB)

    Snchez, A.; Osorio, F.; Ontoso, N.

    2014-07-01

    The FRANX application has been developed by EPRI within the Risk and Reliability User Group in order to facilitate the process of quantification and updating APS Fire (also covers floods and earthquakes). By applying fire scenarios are quantified in the central integrating the tasks performed during the APS fire. This paper describes the main features of the program to allow quantification of an APS Fire. (Author)

  11. AP Studio Art as an Enabling Constraint for Secondary Art Education

    Science.gov (United States)

    Graham, Mark A.

    2009-01-01

    Advanced Placement (AP) Studio Art is an influential force in secondary art education as is evident in the 31,800 portfolios submitted for review in 2008. From the perspectives of a high school educator and AP Reader, this author has observed how the constraints of the AP program can be used to generate support for high school art programs and…

  12. Using a Classroom Response System to Improve Multiple-Choice Performance in AP[R] Physics

    Science.gov (United States)

    Bertrand, Peggy

    2009-01-01

    Participation in rigorous high school courses such as Advanced Placement (AP[R]) Physics increases the likelihood of college success, especially for students who are traditionally underserved. Tackling difficult multiple-choice exams should be part of any AP program because well-constructed multiple-choice questions, such as those on AP exams and…

  13. Structural characterization of the N-terminal mineral modification domains from the molluscan crystal-modulating biomineralization proteins, AP7 and AP24.

    Science.gov (United States)

    Wustman, Brandon A; Morse, Daniel E; Evans, John Spencer

    2004-08-05

    The AP7 and AP24 proteins represent a class of mineral-interaction polypeptides that are found in the aragonite-containing nacre layer of mollusk shell (H. rufescens). These proteins have been shown to preferentially interfere with calcium carbonate mineral growth in vitro. It is believed that both proteins play an important role in aragonite polymorph selection in the mollusk shell. Previously, we demonstrated the 1-30 amino acid (AA) N-terminal sequences of AP7 and AP24 represent mineral interaction/modification domains in both proteins, as evidenced by their ability to frustrate calcium carbonate crystal growth at step edge regions. In this present report, using free N-terminal, C(alpha)-amide "capped" synthetic polypeptides representing the 1-30 AA regions of AP7 (AP7-1 polypeptide) and AP24 (AP24-1 polypeptide) and NMR spectroscopy, we confirm that both N-terminal sequences possess putative Ca (II) interaction polyanionic sequence regions (2 x -DD- in AP7-1, -DDDED- in AP24-1) that are random coil-like in structure. However, with regard to the remaining sequences regions, each polypeptide features unique structural differences. AP7-1 possesses an extended beta-strand or polyproline type II-like structure within the A11-M10, S12-V13, and S28-I27 sequence regions, with the remaining sequence regions adopting a random-coil-like structure, a trait common to other polyelectrolyte mineral-associated polypeptide sequences. Conversely, AP24-1 possesses random coil-like structure within A1-S9 and Q14-N16 sequence regions, and evidence for turn-like, bend, or loop conformation within the G10-N13, Q17-N24, and M29-F30 sequence regions, similar to the structures identified within the putative elastomeric proteins Lustrin A and sea urchin spicule matrix proteins. The similarities and differences in AP7 and AP24 N-terminal domain structure are discussed with regard to joint AP7-AP24 protein modification of calcium carbonate growth.

  14. Plant-specific insertions in the soybean aspartic proteinases, soyAP1 and soyAP2, perform different functions of vacuolar targeting.

    Science.gov (United States)

    Terauchi, Kaede; Asakura, Tomiko; Ueda, Haruko; Tamura, Tomoko; Tamura, Kentaro; Matsumoto, Ichiro; Misaka, Takumi; Hara-Nishimura, Ikuko; Abe, Keiko

    2006-07-01

    Most aspartic proteinases (APs) of plant origin are characterized by the presence of plant-specific insertion (PSI) in their primary structure. PSI has been reported to function as signals for both transport of AP molecules from the endoplasmic reticulum (ER) and for their targeting to the vacuole. To determine the functions of the PSIs in soyAP1 and soyAP2 identified in our previous study, we examined their subcellular localization by transient expression of a green fluorescent protein (GFP) fusion protein in the protoplasts of Arabidopsis suspension-cultured cells. Both soyAP1-GFP and soyAP2-GFP were targeted to the vacuole. To confirm the role of the PSI, we prepared PSI-deleted soyAP1 and soyAP2, and investigated their vacuolar targeting by the same method. While the former deletion mutant was always transported to the vacuole, the latter sometimes remained in the ER and was only sometimes transported to the vacuole. These observations indicated that, in the case of soyAP1, the PSI is not involved in vacuolar targeting, also suggesting that the function of the PSI differs depending on its origin.

  15. Biologi Radiasi

    OpenAIRE

    Milla Yoesfianda

    2008-01-01

    Biologi radiasi adalah ilmu yang mempelajari tentang pengaruh dari ionisasi radiasi dalam tubuh makhluk hidup. Kemungkinan terjadinya efek biologis akibat interaksi radiasi dan jaringan tubuh manusia, berbanding lurus dengan besarnya dosis radiasi yang mengenai jaringan tubuh tersebut. Radiasi dapat mengakibatkan efek baik secara langsung maupun tidak langsung. Efek yang merusak secara biologis dari radiasi ionisasi diklasifikasikan menjadi tiga kategori utama, yaitu efek somatik determin...

  16. Generic effluent monitoring system certification for AP-40 exhauster stack

    Energy Technology Data Exchange (ETDEWEB)

    Glissmeyer, J.A.; Davis, W.E.; Bussell, J.H.; Maughan, A.D.

    1997-09-01

    Tests were conducted to verify that the Generic Effluent Monitoring System (GEMS), as applied to the AP-40 exhauster stack, meets all applicable regulatory performance criteria for air sampling systems at nuclear facilities. These performance criteria address both the suitability of the air sampling probe location and the transport of the sample to the collection devices. The criteria covering air sampling probe location ensure that the contaminants in the stack are well mixed with the airflow at the probe location such that the extracted sample represents the whole. The sample transport criteria ensure that the sampled contaminants are quantitatively delivered to the collection device. The specific performance criteria are described in detail in the report. The tests demonstrated that the GEMS/AP-40 system meets all applicable performance criteria. The contaminant mixing tests were conducted by Pacific Northwest National Laboratory (PNNL) at the wind tunnel facility, 331-H Building, using a mockup of the actual stack. The particle sample transport tests were conducted by PNNL at the Numatec Hanford Company`s 305 Building. The AP-40 stack is typical of several 10-in. diameter stacks that discharge the filtered ventilation air from tank farms at the U.S. Department of Energy`s Hanford Site in Richland, Washington. The GEMS design features a probe with a single shrouded sampling nozzle, a sample delivery line, and sample collection system. The collection system includes a filter holder to collect the sample of record and an in-line detector head and filter for monitoring beta radiation-emitting particles. Unrelated to the performance criteria, it was found that the record sample filter holder exhibited symptoms of sample bypass around the particle collection filter. This filter holder should either be modified or replaced with a different type. 10 refs., 8 figs., 6 tabs.

  17. COMPARISON OF NONLINEAR DYNAMICS OPTIMIZATION METHODS FOR APS-U

    Energy Technology Data Exchange (ETDEWEB)

    Sun, Y.; Borland, Michael

    2017-06-25

    Many different objectives and genetic algorithms have been proposed for storage ring nonlinear dynamics performance optimization. These optimization objectives include nonlinear chromaticities and driving/detuning terms, on-momentum and off-momentum dynamic acceptance, chromatic detuning, local momentum acceptance, variation of transverse invariant, Touschek lifetime, etc. In this paper, the effectiveness of several different optimization methods and objectives are compared for the nonlinear beam dynamics optimization of the Advanced Photon Source upgrade (APS-U) lattice. The optimized solutions from these different methods are preliminarily compared in terms of the dynamic acceptance, local momentum acceptance, chromatic detuning, and other performance measures.

  18. Ethernet ring protection with managed FDB using APS payload

    Science.gov (United States)

    Im, Jinsung; Ryoo, Jeong-dong; Joo, Bheom Soon; Rhee, J.-K. Kevin

    2007-11-01

    Ethernet ring protection (ERP) is a new technology based on OAM (operations, administration, and maintenance) being standardized by the ITU-T G.8032 working group. In this paper, we present the recent development of Ethernet ring protection which is called FDB (filtering database) flush scheme and propose a new Ethernet ring protection technique introducing a managed FDB using APS to deliver information how to fix FDB selectively. We discuss the current development of the ERP technology at ITU-T and performance comparisons between different proposals.

  19. Initial diagnostics commissioning results for the APS injector subsystems

    Science.gov (United States)

    Lumpkin, A.; Chung, Y.; Kahana, E.; Patterson, D.; Sellyey, W.; Smith, T.; Wang, X.

    1995-05-01

    In recent months the first beams have been introduced into the various injector subsystems of the Advanced Photon Source (APS). An overview will be given of the diagnostics results on beam profiling, beam position monitors (BPMs), loss rate monitors (LRMs), current monitors (CMs), and photon monitors on the low energy transport lines, positron accumulator ring (PAR), and injector synchrotron (IS). Initial measurements have been done with electron beams at energies from 250 to 450 MeV and 50 to 400 pC per macrobunch. Operations in single turn and stored beam conditions were diagnosed in the PAR and IS.

  20. Desarrollo y Estudio del protocolo Observe para CoAP

    OpenAIRE

    Gimeno Gimenez, Javier

    2013-01-01

    Premi Accenture al millor Projecte Final de Carrera, Treball Fi de Grau, Master Thesis, en l'Àrea de Serveis Telemàtics (curs 2012-2013) [ANGLÈS] In the context of the wireless sensor networks, an implementation of the observer pattern for CoAP is done. The implementation is done both for the nodes of the network and for a proxy that will allow to establish object-observer relationships between sensors inside the WSN and elements outside the network. [CASTELLÀ] En el contexto de las red...

  1. Operation of the APS photoinjector drive laser system.

    Energy Technology Data Exchange (ETDEWEB)

    Li, Y.; Accelerator Systems Division (APS)

    2008-08-04

    The APS photoinjector drive laser system has been in operation since 1999 and is achieving a performance level exceeding the requirement of stable operation of the LEUTL FEL system. One remarkable number is the UV energy stability of better than 2% rms, sometimes less than 1% rms. This report summarizes the operation experience of the laser system and the improvements made along the way. We also outline the route of upgrade of the system and some frontier laser research and development opportunities in ultrabright electron beam generation.

  2. AP/二茂铁衍生物混合体系的热感度研究%Study on thermal sensitivity of AP and ferrocene derivative mixtures

    Institute of Scientific and Technical Information of China (English)

    王宁; 赵孝彬; 罗岚; 李彦荣; 段卫毅

    2011-01-01

    通过局部热感度试验和DSC-TG联用仪,研究了高氯酸铵(AP)和一种二茂铁衍生物(EMT)混合体系的热感度.结果表明,减小AP粒度或增加EMT含量,能缩小AP高低温分解峰之间的差距,提高AP低温分解量,混合物的局部热感度增加.AP存在下,EMT的侧链容易发生断裂,断裂后的二茂铁或二茂铁离子活性体对AP分解起催化作用.此外,EMT 不利于AP的质子转移过程,但对HClO4的分解有较强催化作用.%Thermal sensitivity of AP and ferrocene derivative (EMT) mixtures was studied by partial thermal sensitivity and DSC-TG coupling. The results indicate that reducing AP particle size or increasing the content of EMT can shorten the gap between high-temperature thermal decomposition peak of AP and low-temperature thermal decomposition peak of AP, enhance the low-temperature thermad decomposition amount of AP,and increase partial thermal sensitivity about mixtures of AP and EMT. In the presence of AP, side-chain of EMT is easily ruptured. Residual main skeleton, ferrocene or ferrocene ion, is the real catalyst to thermal decomposition of AP. Furthermore, EMT inhabits proton transfer process of AP, but it is a strong catalysis of EMT to decomposition of HCIO4, one of initial decomposition product of AP.

  3. NF-κB/AP-1-Targeted Inhibition of Macrophage-Mediated Inflammatory Responses by Depigmenting Compound AP736 Derived from Natural 1,3-Diphenylpropane Skeleton

    Directory of Open Access Journals (Sweden)

    Van Thai Ha

    2014-01-01

    Full Text Available AP736 was identified as an antimelanogenic drug that can be used for the prevention of melasma, freckles, and dark spots in skin by acting as a suppressor of melanin synthesis and tyrosinase expression. Since macrophage-mediated inflammatory responses are critical for skin health, here we investigated the potential anti-inflammatory activity of AP736. The effects of AP736 on various inflammatory events such as nitric oxide (NO/prostaglandin (PG E2 production, inflammatory gene expression, phagocytic uptake, and morphological changes were examined in RAW264.7 cells. AP736 was found to strongly inhibit the production of both NO and PGE2 in lipopolysaccharide- (LPS- treated RAW264.7 cells. In addition, AP736 strongly inhibited both LPS-induced morphological changes and FITC-dextran-induced phagocytic uptake. Furthermore, AP736 also downregulated the expression of multiple inflammatory genes, such as inducible NO synthase (iNOS, cyclooxygenase- (COX- 2, and interleukin- (IL- 1β in LPS-treated RAW264.7 cells. Transcription factor analysis, including upstream signalling events, revealed that both NF-κB and AP-1 were targeted by AP736 via inhibition of the IKK/IκBα and IRAK1/TAK1 pathways. Therefore, our results strongly suggest that AP736 is a potential anti-inflammatory drug due to its suppression of NF-κB-IKK/IκBα and AP-1-IRAK1/TAK1 signalling, which may make AP736 useful for the treatment of macrophage-mediated skin inflammation.

  4. Packaging design criteria, transfer and disposal of 102-AP mixer pump

    Energy Technology Data Exchange (ETDEWEB)

    Carlstrom, R.F.

    1994-11-23

    A mixer pump installed in storage tank 241-AP-102 (102-AP) has failed. This pump is referred to as the 102-AP mixer pump (APMP). The APMP will be removed from 102-AP 1 and a new pump will be installed. The main purpose of the Packaging Design Criteria (PDC) is to establish criteria necessary to design and fabricate a shipping container for the transfer and storage of the APMP from 102-AP. The PDC will be used as a guide to develop a Safety Evaluation for Packaging (SEP).

  5. Overexpression of ligase defective E6-associated protein, E6-AP, results in mammary tumorigenesis.

    Science.gov (United States)

    Ramamoorthy, Sivapriya; Tufail, Rozina; Hokayem, Jimmy El; Jorda, Mercy; Zhao, Wei; Reis, Zizi; Nawaz, Zafar

    2012-02-01

    E6-associated protein (E6-AP) is a dual function protein. It acts as an E3 ubiquitin-protein ligase enzyme and coactivator of steroid hormone receptors such as estrogen (ERα) and progesterone (PR) receptors. It promotes the degradation of ERα and PR through the ubiquitin-proteasome pathway. Furthermore, it has been shown that the levels of E6-AP are inversely associated with that of ERα in human breast tumors. But the role of wild-type human E6-AP and its ubiquitin-protein ligase activity in mammary tumorigenesis is still unknown. To investigate this role, the authors utilized transgenic mice lines that specifically overexpress either the wild-type human E6-AP (E6-AP(WT)) or the ubiquitin-protein ligase defective E6-AP that contains C833S mutation (E6-AP(C833S)) in the mammary gland. To further substantiate the role of E6-AP in the development of breast tumorigenesis, it was also examined the expression of E6-AP in a large cohort of human breast cancer samples. The transgenic mice that overexpress wild-type E6-AP (E6-AP(WT)) fail to develop mammary tumors. Unlike the E6-AP(WT) mice, the E6-AP(C833S) mice that overexpress ubiquitin-protein ligase defective E6-AP protein develop mammary hyperplasia with a median latency of 18 months. These observations suggest that the inactivation of the ubiquitin-protein ligase function of E6-AP is sufficient to initiate the process of mammary tumor development. Furthermore, the data also suggests that E6-AP exerts its effects on target cells by modulating the protein levels and functions of ERα and PR. In addition, it was found in human breast cancer patients that the level of E6-AP is decreased in invasive breast tumors compared to normal breast tissue. Moreover, the authors also show that the survival patterns for E6-AP negative patients were worse compared to E6-AP positive patients. Taken together, these data suggests that E6-AP may act as a tumor suppressor in breast.

  6. [Biological weapons].

    Science.gov (United States)

    Kerwat, K; Becker, S; Wulf, H; Densow, D

    2010-08-01

    Biological weapons are weapons of mass destruction that use pathogens (bacteria, viruses) or the toxins produced by them to target living organisms or to contaminate non-living substances. In the past, biological warfare has been repeatedly used. Anthrax, plague and smallpox are regarded as the most dangerous biological weapons by various institutions. Nowadays it seems quite unlikely that biological warfare will be employed in any military campaigns. However, the possibility remains that biological weapons may be used in acts of bioterrorism. In addition all diseases caused by biological weapons may also occur naturally or as a result of a laboratory accident. Risk assessment with regard to biological danger often proves to be difficult. In this context, an early identification of a potentially dangerous situation through experts is essential to limit the degree of damage. Georg Thieme Verlag KG Stuttgart * New York.

  7. Mathematical biology

    CERN Document Server

    Murray, James D

    1993-01-01

    The book is a textbook (with many exercises) giving an in-depth account of the practical use of mathematical modelling in the biomedical sciences. The mathematical level required is generally not high and the emphasis is on what is required to solve the real biological problem. The subject matter is drawn, e.g. from population biology, reaction kinetics, biological oscillators and switches, Belousov-Zhabotinskii reaction, reaction-diffusion theory, biological wave phenomena, central pattern generators, neural models, spread of epidemics, mechanochemical theory of biological pattern formation and importance in evolution. Most of the models are based on real biological problems and the predictions and explanations offered as a direct result of mathematical analysis of the models are important aspects of the book. The aim is to provide a thorough training in practical mathematical biology and to show how exciting and novel mathematical challenges arise from a genuine interdisciplinary involvement with the biosci...

  8. Molecular Basis for Enhancement of the Meiotic DMCI Recombinase by RAD51AP1

    Energy Technology Data Exchange (ETDEWEB)

    Dray, Eloise; Dunlop, Myun Hwa; Kauppi, Liisa; San Filippo, Joseph San; Wiese, Claudia; Tsai, Miaw-Sheue; Begovic, Sead; Schild, David; Jasin, Maria; Keeney, Scott; Sung, Patrick

    2010-11-05

    Homologous recombination is needed for meiotic chromosome segregation, genome maintenance, and tumor suppression. RAD51AP1 (RAD51 Associated Protein 1) has been shown to interact with and enhance the recombinase activity of RAD51. Accordingly, genetic ablation of RAD51AP1 leads to enhanced sensitivity to and also chromosome aberrations upon DNA damage, demonstrating a role for RAD51AP1 in mitotic homologous recombination. Here we show physical association of RAD51AP1 with the meiosis-specific recombinase DMC1 and a stimulatory effect of RAD51AP1 on the DMC1-mediated D-loop reaction. Mechanistic studies have revealed that RAD51AP1 enhances the ability of the DMC1 presynaptic filament to capture the duplex DNA partner and to assemble the synaptic complex, in which the recombining DNA strands are homologously aligned. We also provide evidence that functional co-operation is dependent on complex formation between DMC1 and RAD51AP1, and that distinct epitopes in RAD51AP1 mediate interactions with RAD51 and DMC1. Finally, we show that RAD51AP1 is expressed in mouse testes, and that RAD51AP1 foci co-localize with a subset of DMC1 foci in spermatocytes. These results suggest that RAD51AP1 also serves an important role in meiotic homologous recombination.

  9. Lack of CD2AP disrupts Glut4 trafficking and attenuates glucose uptake in podocytes.

    Science.gov (United States)

    Tolvanen, Tuomas A; Dash, Surjya Narayan; Polianskyte-Prause, Zydrune; Dumont, Vincent; Lehtonen, Sanna

    2015-12-15

    The adapter protein CD2-associated protein (CD2AP) functions in various signaling and vesicle trafficking pathways, including endosomal sorting and/or trafficking and degradation pathways. Here, we investigated the role of CD2AP in insulin-dependent glucose transporter 4 (Glut4, also known as SLC2A4) trafficking and glucose uptake. Glucose uptake was attenuated in CD2AP(-/-) podocytes compared with wild-type podocytes in the basal state, and CD2AP(-/-) podocytes failed to increase glucose uptake in response to insulin. Live-cell imaging revealed dynamic trafficking of HA-Glut4-GFP in wild-type podocytes, whereas in CD2AP(-/-) podocytes, HA-Glut4-GFP clustered perinuclearly. In subcellular membrane fractionations, CD2AP co-fractionated with Glut4, IRAP (also known as LNPEP) and sortilin, constituents of Glut4 storage vesicles (GSVs). We further found that CD2AP forms a complex with GGA2, a clathrin adaptor, which sorts Glut4 to GSVs, suggesting a role for CD2AP in this process. We also found that CD2AP forms a complex with clathrin and connects clathrin to actin in the perinuclear region. Furthermore, clathrin recycling back to trans-Golgi membranes from the vesicular fraction containing GSVs was defective in the absence of CD2AP. This leads to reduced insulin-stimulated trafficking of GSVs and attenuated glucose uptake into CD2AP(-/-) podocytes.

  10. Performance of the APS optical slope measuring system

    Energy Technology Data Exchange (ETDEWEB)

    Qian, Jun, E-mail: jqian@aps.anl.gov [Advanced Photon Source, Argonne National Laboratory, 9700S. Cass Avenue, Argonne, 60439 (United States); Sullivan, Joe; Erdmann, Mark; Khounsary, Ali; Assoufid, Lahsen [Advanced Photon Source, Argonne National Laboratory, 9700S. Cass Avenue, Argonne, 60439 (United States)

    2013-05-11

    An optical slope measuring system (OSMS) was recently brought into operation at the Advanced Photon Source of the Argonne National Laboratory. This system is equipped with a precision autocollimator and a very accurate mirror-based pentaprism on a scanning stage and kept in an environment-controlled enclosure. This system has the capability to measure precision optics with sub-microradian rms slope errors as documented with a series of tests demonstrating accuracy, stability, reliability and repeatability. Measurements of a flat mirror with 0.2 μrad rms slope error are presented which show that the variation of the slope profile measurements with the mirror setting at different locations along the scanning direction is only 60 nrad and the corresponding height error profile has 2 nm rms. -- Highlights: ► This is the first time to present the APS OSMS in publication. ► The APS OSMS is capable to measure flat and near flat mirrors with slope error <100 nrad rms. ► The accuracy of the slope error measurements of a 350 mm long mirror is less than 60 nrad rms.

  11. The new AP Physics exams: Integrating qualitative and quantitative reasoning

    Science.gov (United States)

    Elby, Andrew

    2015-04-01

    When physics instructors and education researchers emphasize the importance of integrating qualitative and quantitative reasoning in problem solving, they usually mean using those types of reasoning serially and separately: first students should analyze the physical situation qualitatively/conceptually to figure out the relevant equations, then they should process those equations quantitatively to generate a solution, and finally they should use qualitative reasoning to check that answer for plausibility (Heller, Keith, & Anderson, 1992). The new AP Physics 1 and 2 exams will, of course, reward this approach to problem solving. But one kind of free response question will demand and reward a further integration of qualitative and quantitative reasoning, namely mathematical modeling and sense-making--inventing new equations to capture a physical situation and focusing on proportionalities, inverse proportionalities, and other functional relations to infer what the equation ``says'' about the physical world. In this talk, I discuss examples of these qualitative-quantitative translation questions, highlighting how they differ from both standard quantitative and standard qualitative questions. I then discuss the kinds of modeling activities that can help AP and college students develop these skills and habits of mind.

  12. Pulsation models for the roAp star HD 134214

    CERN Document Server

    Saio, H; Weiss, W W; Matthews, J M; Ryabchikova, T

    2011-01-01

    Precise time-series photometry with the MOST satellite has led to identification of 10 pulsation frequencies in the rapidly oscillating Ap (roAp) star HD 134214. We have fitted the observed frequencies with theoretical frequencies of axisymmetric modes in a grid of stellar models with dipole magnetic fields. We find that, among models with a standard composition of $(X,Z) = (0.70,0.02)$ and with suppressed convection, eigenfrequencies of a $1.65\\,{\\rm M}_\\odot$ model with $\\log T_{\\rm eff} = 3.858$ and a polar magnetic field strength of 4.1kG agree best with the observed frequencies. We identify the observed pulsation frequency with the largest amplitude as a deformed dipole ($\\ell = 1$) mode, and the four next-largest-amplitude frequencies as deformed $\\ell = 2$ modes. These modes have a radial quasi-node in the outermost atmospheric layers ($\\tau \\sim 10^{-3}$). Although the model frequencies agree roughly with observed ones, they are all above the acoustic cut-off frequency for the model atmosphere and hen...

  13. Apsáalooke women's experiences with Pap test screening.

    Science.gov (United States)

    Smith, Adina J; Christopher, Suzanne; LaFromboise, Victoria R; Letiecq, Bethany L; McCormick, Alma Knows His Gun

    2008-04-01

    Cervical cancer mortality rates are among the highest in the United States for Northern Plains Native American women compared with white and other Native American women. The aims of Messengers for Health, a community-based participatory research project based on the Apsáalooke (Crow Indian) Reservation, are to decrease cervical cancer screening barriers, improve knowledge regarding screening and prevention, and increase the proportion of women receiving Pap tests. This paper presents results from a survey assessing women's perceptions of the level of comfort and care received by health care providers in their most recent Pap test appointment. A survey assessing patient communication and satisfaction with their health care providers was conducted with a random sample of 101 Apsáalooke women. Qualitative and quantitative methods were utilized to analyze the survey data. Women reported both positive and negative experiences with their provider regarding their Pap test appointments. They noted positive experiences when trust was established and when the provider offered information, reassured or encouraged them, was personable, was familiar or consistent, maintained confidentiality, and was a woman. The women reported negative experiences when the examination was too short, when they did not have a consistent or female provider, and when they did not feel comfortable with the provider's nonverbal communication. Continued work with both providers and patients is necessary to decrease communication barriers and increase satisfaction with Pap test appointments.

  14. Luteolin, a flavonoid, inhibits AP-1 activation by basophils.

    Science.gov (United States)

    Hirano, Toru; Higa, Shinji; Arimitsu, Junsuke; Naka, Tetsuji; Ogata, Atsushi; Shima, Yoshihito; Fujimoto, Minoru; Yamadori, Tomoki; Ohkawara, Tomoharu; Kuwabara, Yusuke; Kawai, Mari; Matsuda, Hisashi; Yoshikawa, Masayuki; Maezaki, Naoyoshi; Tanaka, Tetsuaki; Kawase, Ichiro; Tanaka, Toshio

    2006-02-03

    Flavonoids including luteolin, apigenin, and fisetin are inhibitors of IL-4 synthesis and CD40 ligand expression by basophils. This study was done to search for compounds with greater inhibitory activity of IL-4 expression and to clarify the molecular mechanisms through which flavonoids inhibit their expression. Of the 37 flavonoids and related compounds examined, ayanin, luteolin, and apigenin were the strongest inhibitors of IL-4 production by purified basophils in response to anti-IgE antibody plus IL-3. Luteolin did not suppress Syk or Lyn phosphorylation in basophils, nor did suppress p54/46 SAPK/JNK, p38 MAPK, and p44/42 MAPK activation by a basophilic cell line, KU812 cells, stimulated with A23187 and PMA. However, luteolin did inhibit phosphorylation of c-Jun and DNA binding activity of AP-1 in nuclear lysates from stimulated KU812 cells. These results provide a fundamental structure of flavonoids for IL-4 inhibition and demonstrate a novel action of flavonoids that suppresses the activation of AP-1.

  15. Data Aggregation Gateway Framework for CoAP Group Communications

    Directory of Open Access Journals (Sweden)

    Minki Cha

    2016-11-01

    Full Text Available In this paper, a data aggregation gateway framework (DA-GW for constrained application protocol (CoAP group communications is proposed. The DA-GW framework is designed to improve the throughput performance and energy efficiency of group communication to monitor and control multiple sensor devices collectively with a single user terminal. The DA-GW consists of four function blocks—the message analyzer, group manager, message scheduler and data handler—and three informative databases—the client database, resource database and information database. The DA-GW performs group management and group communication through each functional block and stores resources in the informative databases. The DA-GW employs international standard-based data structures and provides the interoperability of heterogeneous devices used in various applications. The DA-GW is implemented using a Java-based open source framework called jCoAP to evaluate the functions and performance of the DA-GW. The experiment results showed that the DA-GW framework revealed better performance than existing group communication methods in terms of throughput and energy consumption.

  16. Abnormal lithium abundance in several Ap-Bp stars

    CERN Document Server

    Shavrina, A; Drake, N A; Kudryavtsev, D O; Gopka, V F; Yushchenko, V A; Yushchenko, A V

    2013-01-01

    Using observations of rapidly rotating roAp star HD 12098, obtained with 6m telescope of SAO RAS in 2005-2008 in nine rotational phases (0.001-0.817), and code V. Tsymbal ROTATE we identified 3 lithium spots on the surface of star, one of them coincided with the position of one magnetic pole and two others are located near the other pole, symmetrically with respect to it. Perhaps magnetic field of the star has a more complex structure than the dipole (Shavrina 2008). Lithium abundance in the spots is greater than cosmic one. It can be explained by diffusion of lithium in the presence of a magnetic field or by production of lithium in the reactions of "spallation" on the surface of star near the magnetic poles (see, for example, Goriely 2007). Enhanced lithium abundances was also determined for other two slowly rotating Ap stars HR 465 and HD 965. The analysis is performed using a synthetic spectrum method taking into account magnetic splitting of lines by the SYNTHM code of Khan (2004).

  17. Identification of Cargo for Adaptor Protein (AP) Complexes 3 and 4 by Sucrose Gradient Profiling.

    Science.gov (United States)

    Pertl-Obermeyer, Heidi; Wu, Xu Na; Schrodt, Jens; Müdsam, Christina; Obermeyer, Gerhard; Schulze, Waltraud X

    2016-09-01

    Intracellular vesicle trafficking is a fundamental process in eukaryotic cells. It enables cellular polarity and exchange of proteins between subcellular compartments such as the plasma membrane or the vacuole. Adaptor protein complexes participate in the vesicle formation by specific selection of the transported cargo. We investigated the role of the adaptor protein complex 3 (AP-3) and adaptor protein complex 4 (AP-4) in this selection process by screening for AP-3 and AP-4 dependent cargo proteins. Specific cargo proteins are expected to be mis-targeted in knock-out mutants of adaptor protein complex components. Thus, we screened for altered distribution profiles across a density gradient of membrane proteins in wild type versus ap-3β and ap-4β knock-out mutants. In ap-3β mutants, especially proteins with transport functions, such as aquaporins and plasma membrane ATPase, as well as vesicle trafficking proteins showed differential protein distribution profiles across the density gradient. In the ap-4β mutant aquaporins but also proteins from lipid metabolism were differentially distributed. These proteins also showed differential phosphorylation patterns in ap-3β and ap-4β compared with wild type. Other proteins, such as receptor kinases were depleted from the AP-3 mutant membrane system, possibly because of degradation after mis-targeting. In AP-4 mutants, membrane fractions were depleted for cytochrome P450 proteins, cell wall proteins and receptor kinases. Analysis of water transport capacity in wild type and mutant mesophyll cells confirmed aquaporins as cargo proteins of AP-3 and AP-4. The combination of organelle density gradients with proteome analysis turned out as a suitable experimental strategy for large-scale analyses of protein trafficking.

  18. RAD51AP1-deficiency in vertebrate cells impairs DNA replication.

    Science.gov (United States)

    Parplys, Ann C; Kratz, Katja; Speed, Michael C; Leung, Stanley G; Schild, David; Wiese, Claudia

    2014-12-01

    RAD51-associated protein 1 (RAD51AP1) is critical for homologous recombination (HR) by interacting with and stimulating the activities of the RAD51 and DMC1 recombinases. In human somatic cells, knockdown of RAD51AP1 results in increased sensitivity to DNA damaging agents and to impaired HR, but the formation of DNA damage-induced RAD51 foci is unaffected. Here, we generated a genetic model system, based on chicken DT40 cells, to assess the phenotype of fully inactivated RAD51AP1 in vertebrate cells. Targeted inactivation of both RAD51AP1 alleles has no effect on either viability or doubling-time in undamaged cells, but leads to increased levels of cytotoxicity after exposure to cisplatin or to ionizing radiation. Interestingly, ectopic expression of GgRAD51AP1, but not of HsRAD51AP1 is able to fully complement in cell survival assays. Notably, in RAD51AP1-deficient DT40 cells the resolution of DNA damage-induced RAD51 foci is greatly slowed down, while their formation is not impaired. We also identify, for the first time, an important role for RAD51AP1 in counteracting both spontaneous and DNA damage-induced replication stress. In human and in chicken cells, RAD51AP1 is required to maintain wild type speed of replication fork progression, and both RAD51AP1-depleted human cells and RAD51AP1-deficient DT40 cells respond to replication stress by a slow-down of replication fork elongation rates. However, increased firing of replication origins occurs in RAD51AP1-/- DT40 cells, likely to ensure the timely duplication of the entire genome. Taken together, our results may explain why RAD51AP1 commonly is overexpressed in tumor cells and tissues, and we speculate that the disruption of RAD51AP1 function could be a promising approach in targeted tumor therapy.

  19. Expansion and Functional Divergence of AP2 Group Genes in Spermatophytes Determined by Molecular Evolution and Arabidopsis Mutant Analysis

    Directory of Open Access Journals (Sweden)

    Pengkai Wang

    2016-09-01

    Full Text Available The APETALA2 (AP2 genes represent the AP2 group within a large group of DNA-binding proteins called AP2/EREBP. The AP2 gene is functional and necessary for flower development, stem cell maintenance, and seed development, whereas the other members of AP2 group redundantly affect flowering time. Here we study the phylogeny of AP2 group genes in spermatophytes. Spermatophyte AP2 group genes can be classified into AP2 and TOE types, six clades, and we found that the AP2 group homologs in gymnosperms belong to the AP2 type, whereas TOE types are absent, which indicates the AP2 type gene are more ancient and TOE type was split out of AP2 type and losing the major function. In Brassicaceae, the expansion of AP2 and TOE type lead to the gene number of AP2 group were up to six. Purifying selection appears to have been the primary driving force of spermatophyte AP2 group evolution, although positive selection occurred in the AP2 clade. The transition from exon to intron of AtAP2 in Arabidopsis mutant leads to the loss of gene function and the same situation was found in AtTOE2. Combining this evolutionary analysis and published research, the results suggest that typical AP2 group genes may first appear in gymnosperms and diverged in angiosperms, following expansion of group members and functional differentiation. In angiosperms, AP2 genes (AP2 clade inherited key functions from ancestors and other genes of AP2 group lost most function but just remained flowering time controlling in gene formation. In this study, the phylogenies of AP2 group genes in spermatophytes was analyzed, which supported the evidence for the research of gene functional evolution of AP2 group.

  20. 储氢合金/AP/HTPB推进剂燃烧性能研究%Investigation on Combustion Properties of Hydrogen Storage Alloy/AP/HTPB Propellant

    Institute of Scientific and Technical Information of China (English)

    窦燕蒙; 罗运军; 李国平; 葛震

    2013-01-01

    AI/AP/HTPB propellant and hydrogen storage alloy/AP/HTPB propellant were prepared and their properties were characterized by DSC,PDSC,explosion heat and burning rate test. DSC and PDSC test results show that hydrogen storage alloy has catalysis on condensed phase reactions of AP/HTPB propellant in which main decomposition temperature is decreased by 21.67℃,and 15. 67 ℃ at 0. 1MPa and 5MPa,respectively while condensed phase reaction heat is increased by 105. 97% and 21. 87% separately. Explosion heat test results show that hydrogen storage alloy has catalysis on gas phase reactions of AP/HTPB propellant in which gas phase reaction heat is increased by 4. 74% . Burning rate test results show that hydrogen storage alloy can increase burning rate of AP/HTPB propellant. Further studies indicate that possible correlation exist between burning rate and explosion heat of Al/AP/HTPB propellant and hydrogen storage alloy/AP/HTPB propellant. The burning rate-explosion heat formula is summed up.%制备了Al/AP/HTPB推进剂和储氢合金/AP/HTPB推进剂,并对它们进行了DSC,PDSC,爆热和燃速测试.DSC和PDSC测试结果表明,储氢合金对AP/HTPB推进剂的凝聚相反应有催化作用,在0.1MPa和5MPa下,使其凝聚相主分解温度分别降低21.67℃和15.67℃,凝聚相反应热分别提高105.97%和21.87%;爆热测试结果表明,储氢合金对AP/HTPB推进剂的气相反应有催化作用,使气相反应热提高4.74%;燃速结果表明,储氢合金可以提高AP/HTPB推进剂的燃速.进一步研究表明,A1/AP/HTPB推进剂和储氢合金/AP/HTPB推进剂的燃速与爆热存在一定的相关性.总结出了燃速-爆热关系式.

  1. Constitutive Expression of Sense & Antisense PtAP3, an AP3 Homologue Gene of Populus tomentosa, Affects Growth and Flowering Time in Transgenic Tobacco

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    To analyze the function of PtAP3, an APETALA3 (AP3) homologue gene isolated from Populus tomentosa Carr., the full length sequence (1 797 bp) and a fragment (870 bp) of PtAP3 were fused to a CaMV 35S promoter of pBI121 to generate the sense and antisense constructs of PtAP3. These constructs were transformed into tobacco by Agrobacterium infection of leaf disks and selection on kanamycin medium. Some sense and antisense transgenic tobacco plants were obtained by PCR and Southern blot analysis. Great phenotypic differences in transgenic tobacco plants were observed. Almost all of sense PtAP3 to transgenic tobaccos showed a higher growth rate than those of antisense transformants and a few developed pregnancy earlier than wild type seedlings and antisense transformants under the same conditions.

  2. RAD51AP2, a novel vertebrate- and meiotic-specific protein, sharesa conserved RAD51-interacting C-terminal domain with RAD51AP1/PIR51

    Energy Technology Data Exchange (ETDEWEB)

    Kovalenko, Oleg V.; Wiese, Claudia; Schild, David

    2006-07-25

    Many interacting proteins regulate and/or assist the activities of RAD51, a recombinase which plays a critical role in both DNA repair and meiotic recombination. Yeast two-hybrid screening of a human testis cDNA library revealed a new protein, RAD51AP2 (RAD51 Associated Protein 2), that interacts strongly with RAD51. A full-length cDNA clone predicts a novel vertebrate specific protein of 1159 residues, and the RAD51AP2 transcript was observed only in meiotic tissue (i.e. adult testis and fetal ovary), suggesting a meiotic-specific function for RAD51AP2. In HEK293 cells the interaction of RAD51 with an ectopically-expressed recombinant large fragment of RAD51AP2 requires the C-terminal 57 residues of RAD51AP2. This RAD51-binding region shows 81% homology to the C-terminus of RAD51AP1/PIR51, an otherwise totally unrelated RAD51-binding partner that is ubiquitously expressed. Analyses using truncations and point mutations in both RAD51AP1 and RAD51AP2 demonstrate that these proteins use the same structural motif for RAD51 binding. RAD54 shares some homology with this RAD51-binding motif, but this homologous region plays only an accessory role to the adjacent main RAD51-interacting region, which has been narrowed here to 40 amino acids. A novel protein, RAD51AP2, has been discovered that interacts with RAD51 through a C-terminal motif also present in RAD51AP1.

  3. Duplication of AP1 within the Spinacia oleracea L. AP1/FUL clade is followed by rapid amino acid and regulatory evolution.

    Science.gov (United States)

    Sather, D Noah; Golenberg, Edward M

    2009-02-01

    The AP1/FUL clade of MADS box genes have undergone multiple duplication events among angiosperm species. While initially identified as having floral meristem identity and floral organ identity function in Arabidopsis, the role of AP1 homologs does not appear to be universally conserved even among eudicots. In comparison, the role of FRUITFULL has not been extensively explored in non-model species. We report on the isolation of three AP1/FUL genes from cultivated spinach, Spinacia oleracea L. Two genes, designated SpAPETALA1-1 (SpAP1-1) and SpAPETALA1-2 (SpAP1-2), cluster as paralogous genes within the Caryophyllales AP1 clade. They are highly differentiated in the 3', carboxyl-end encoding region of the gene following the third amphipathic alpha-helix region, while still retaining some elements of a signature AP1 carboxyl motifs. In situ hybridization studies also demonstrate that the two paralogs have evolved different temporal and spatial expression patterns, and that neither gene is expressed in the developing sepal whorl, suggesting that the AP1 floral organ identity function is not conserved in spinach. The spinach FRUITFULL homolog, SpFRUITFULL (SpFUL), has retained the conserved motif and groups with Caryophyllales FRUITFULL homologs. SpFUL is expressed in leaf as well as in floral tissue, and shows strong expression late in flower development, particularly in the tapetal layer in males, and in the endothecium layer and stigma, in the females. The combined evidence of high rates of non-synonymous substitutions and differential expression patterns supports a scenario in which the AP1 homologs in the spinach AP1/FUL gene family have experienced rapid evolution following duplication.

  4. Antisense RNA decreases AP33 gene expression and cytoadherence by T. vaginalis

    Science.gov (United States)

    Mundodi, V; Kucknoor, AS; Alderete, JF

    2007-01-01

    Background Host parasitism by Trichomonas vaginalis is complex. Adherence to vaginal epithelial cells (VECs) is mediated by surface proteins. We showed before that antisense down-regulation of expression of adhesin AP65 decreased amounts of protein, which lowered levels of T. vaginalis adherence to VECs. We now perform antisense down-regulation of expression of the ap33 gene to evaluate and confirm a role for AP33 in adherence by T. vaginalis. We also used an established transfection system for heterologous expression of AP33 in T. foetus as an additional confirmatory approach. Results We successfully select stable trichomonads with sense (S) and antisense (AS) plasmids. RT-PCR confirmed decreased amounts of ap33 mRNA in AS-transfected parasites, and decreased amounts of AP33 had no effect on growth and viability when compared to wild-type (wt) trichomonads. Immunoblots of proteins from AS-transfectants gave significant decreased amounts of functional AP33 capable of binding to host cells compared to wt- and S-transfected trichomonads. As expected, AS-transfectants had lower levels of adherence to VECs, which was related to reduction in surface expression of AP33. Stable expression of T. vaginalis AP33::HA fusion in T. foetus was confirmed by immunoblots and fluorescence. The episomally-expressed surface AP33::HA fusion increased adherence of trichomonads to human VECs, which was abrogated with anti-AP33 serum. Conclusion These results using both antisense inhibition of gene expression and AP33 synthesis and the heterologous expression of AP33 in T. foetus confirms a role for this protein as an adhesin in T. vaginalis. PMID:17608941

  5. Antisense RNA decreases AP33 gene expression and cytoadherence by T. vaginalis

    Directory of Open Access Journals (Sweden)

    Alderete JF

    2007-07-01

    Full Text Available Abstract Background Host parasitism by Trichomonas vaginalis is complex. Adherence to vaginal epithelial cells (VECs is mediated by surface proteins. We showed before that antisense down-regulation of expression of adhesin AP65 decreased amounts of protein, which lowered levels of T. vaginalis adherence to VECs. We now perform antisense down-regulation of expression of the ap33 gene to evaluate and confirm a role for AP33 in adherence by T. vaginalis. We also used an established transfection system for heterologous expression of AP33 in T. foetus as an additional confirmatory approach. Results We successfully select stable trichomonads with sense (S and antisense (AS plasmids. RT-PCR confirmed decreased amounts of ap33 mRNA in AS-transfected parasites, and decreased amounts of AP33 had no effect on growth and viability when compared to wild-type (wt trichomonads. Immunoblots of proteins from AS-transfectants gave significant decreased amounts of functional AP33 capable of binding to host cells compared to wt- and S-transfected trichomonads. As expected, AS-transfectants had lower levels of adherence to VECs, which was related to reduction in surface expression of AP33. Stable expression of T. vaginalis AP33::HA fusion in T. foetus was confirmed by immunoblots and fluorescence. The episomally-expressed surface AP33::HA fusion increased adherence of trichomonads to human VECs, which was abrogated with anti-AP33 serum. Conclusion These results using both antisense inhibition of gene expression and AP33 synthesis and the heterologous expression of AP33 in T. foetus confirms a role for this protein as an adhesin in T. vaginalis.

  6. Is synthetic biology mechanical biology?

    Science.gov (United States)

    Holm, Sune

    2015-12-01

    A widespread and influential characterization of synthetic biology emphasizes that synthetic biology is the application of engineering principles to living systems. Furthermore, there is a strong tendency to express the engineering approach to organisms in terms of what seems to be an ontological claim: organisms are machines. In the paper I investigate the ontological and heuristic significance of the machine analogy in synthetic biology. I argue that the use of the machine analogy and the aim of producing rationally designed organisms does not necessarily imply a commitment to mechanical biology. The ideal of applying engineering principles to biology is best understood as expressing recognition of the machine-unlikeness of natural organisms and the limits of human cognition. The paper suggests an interpretation of the identification of organisms with machines in synthetic biology according to which it expresses a strategy for representing, understanding, and constructing living systems that are more machine-like than natural organisms.

  7. Computational biology

    DEFF Research Database (Denmark)

    Hartmann, Lars Røeboe; Jones, Neil; Simonsen, Jakob Grue

    2011-01-01

    Computation via biological devices has been the subject of close scrutiny since von Neumann’s early work some 60 years ago. In spite of the many relevant works in this field, the notion of programming biological devices seems to be, at best, ill-defined. While many devices are claimed or proved t...

  8. Measurement of sextupole orbit offsets in the APS storage ring.

    Energy Technology Data Exchange (ETDEWEB)

    Borland, M.

    1999-04-16

    Horizontal orbit errors at the sextuples in the Advanced Photon Source (APS) storage ring can cause changes in tune and modulation of the beta functions around the ring. To determine the significance of these effects requires knowing the orbit relative to the magnetic center of the sextuples. The method considered here to determine the horizontal beam position in a given sextupole is to measure the tune shift caused by a change in the sextupole strength. The tune shift and a beta function for the same plane uniquely determine the horizontal beam position in the sextupole. The beta function at the sextupole was determined by propagating the beta functions measured at nearby quadrupoles to the sextupole location. This method was used to measure the sextupole magnetic center offset relative to an adjacent beam position monitor (BPM) at a number of sextupole locations. We report on the successes and problems of the method as well as an improved method.

  9. The APS intranet as a man-machine interface.

    Energy Technology Data Exchange (ETDEWEB)

    Ciarlette, D.; Gerig, R.; McDowell, W.

    1997-12-02

    The Advanced Photon Source at Argonne National Laboratory has implemented a number of methods for people to interact with the accelerator systems. The accelerator operators use Sun workstations running MEDM and WCL to interface interactively with the accelerator, however, many people need to view information rather than interact with the machine. One of the most common interfaces for viewing information at the Advanced Photon Source is the World Wide Web. Information such as operations logbook entries, machine status updates, and displays of archived and current data are easily available to APS personnel. This interface between people and the accelerator has proven to be quite useful. Because the Intranet is operating-system independent and inherently unidirectional, ensuring the prevention of unauthorized or accidental control of the accelerators is straightforward.

  10. SIMULATIONS OF BOOSTER INJECTION EFFICIENCY FOR THE APS-UPGRADE

    Energy Technology Data Exchange (ETDEWEB)

    Calvey, J.; Borland, M.; Harkay, K.; Lindberg, R.; Yao, C.-Y.

    2017-06-25

    The APS-Upgrade will require the injector chain to provide high single bunch charge for swap-out injection. One possible limiting factor to achieving this is an observed reduction of injection efficiency into the booster synchrotron at high charge. We have simulated booster injection using the particle tracking code elegant, including a model for the booster impedance and beam loading in the RF cavities. The simulations point to two possible causes for reduced efficiency: energy oscillations leading to losses at high dispersion locations, and a vertical beam size blowup caused by ions in the Particle Accumulator Ring. We also show that the efficiency is much higher in an alternate booster lattice with smaller vertical beta function and zero dispersion in the straight sections.

  11. Aneurisma gigante del apéndice auricular izquierdo

    OpenAIRE

    Francisco L. Moreno-Martínez; González Alfonso,Osvaldo; Lagomasino Hidalgo,Álvaro L; González Díaz,Alejandro; Oliva Céspedes,Carlos; López Bernal,Omaida J

    2006-01-01

    Los aneurismas de la aurícula izquierda son raros y pueden ser congénitos o adquiridos. Los que interesan la pared libre o el apéndice auricular son entidades más raras aún, hasta 2002 sólo existían 49 casos reportados en la aurícula izquierda y 8 en la derecha. La manifestación clínica más frecuente es la aparición de arritmias auriculares incesantes o recurrentes y pueden presentarse embolias sistémicas que pueden dar al traste con la vida del paciente. Presentamos el caso de una paciente a...

  12. Fabrication and Testing of Deflecting Cavities for APS

    Energy Technology Data Exchange (ETDEWEB)

    Mammosser, John; Wang, Haipeng; Rimmer, Robert; Jim, Henry; Katherine, Wilson; Dhakal, Pashupati; Ali, Nassiri; Jim, Kerby; Jeremiah, Holzbauer; Genfa, Wu; Joel, Fuerst; Yawei, Yang; Zenghai, Li

    2013-09-01

    Jefferson Lab (Newport News, Virginia) in collaboration with Argonne National Laboratory (Argonne, IL) has fabricated and tested four first article, 2.8 GHz, deflecting SRF cavities, for Argonne's Short-Pulse X-ray (SPX) project. These cavities are unique in many ways including the fabrication techniques in which the cavity cell and waveguides were fabricated. These cavity subcomponents were milled from bulk large grain niobium ingot material directly from 3D CAD files. No forming of sub components was used with the exception of the beam-pipes. The challenging cavity and helium vessel design and fabrication results from the stringent RF performance requirements required by the project and operation in the APS ring. Production challenges and fabrication techniques as well as testing results will be discussed in this paper.

  13. AP fuels and the potential of renewable diesel

    Energy Technology Data Exchange (ETDEWEB)

    Berkley, Mark; Seifkar, Navid; O' Shea, Michael; Peters, Christopher

    2010-09-15

    The decrease in demand for forestry products has been detrimental to the Province of Quebec's industrial base. With increasing energy security and environmental concerns the promotion of innovative technologies is adamant. AP Fuels Inc. has undertaken the development of a biomass-to-liquids facility proposed herein as a hybrid design, combining biomass and natural gas capable of producing diesel and other liquid fuels. The facility would consume 2,200,000 tonnewet per year of biomass and produce 10,600 bbl/day of liquid fuels. Forestry-derived F-T fuels have notable advantages including: improved performance; ultra-low sulphur content; reduced emissions, particulates and fouling; and production of fewer by-products.

  14. Towards a theory of rapidly oscillating Ap stars

    CERN Document Server

    Gough, Douglas

    2011-01-01

    Peculiar A stars are so named because they exhibit abundance peculiarities in their atmospheres. It is believed that these arise as a result of differentiation of chemical species in large magnetic spots in which convective mixing is inhibited: there might be just two antipodal spots, whose axis is inclined to the axis of rotation. Many of the Ap stars that are rotating slowly also pulsate, with periods substantially shorter than the period of the fundamental radial mode. The pulsations appear to be nonradial, but axisymmetric, with their common axis usually aligned with the axis of the spots. In this lecture I shall first discuss the magnetic suppression of convection in the spots, and then I shall try to explain the pulsation phenomenon, reviewing some of the suggestions that have been made to explain the alignment and the excitation mechanism, and finally raising some issues that need to be addressed.

  15. Aerosol Polarimetry Sensor (APS): Design Summary, Performance and Potential Modifications

    Science.gov (United States)

    Cairns, Brian

    2014-01-01

    APS is a mature design that has already been built and has a TRL of 7. Algorithmic and retrieval capabilities continue to improve and make better and more sophisticated used of the data. Adjoint solutions, both in one dimensional and three dimensional are computationally efficient and should be the preferred implementation for the calculation of Jacobians (one dimensional), or cost-function gradients (three dimensional). Adjoint solutions necessarily provide resolution of internal fields and simplify incorporation of active measurements in retrievals, which will be necessary for a future ACE mission. Its best to test these capabilities when you know the answer: OSSEs that are well constrained observationally provide the best place to test future multi-instrument platform capabilities and ensure capabilities will meet scientific needs.

  16. Enhanced Antimicrobial Activity of AamAP1-Lysine, a Novel Synthetic Peptide Analog Derived from the Scorpion Venom Peptide AamAP1

    Directory of Open Access Journals (Sweden)

    Ammar Almaaytah

    2014-04-01

    Full Text Available There is great interest in the development of antimicrobial peptides as a potentially novel class of antimicrobial agents. Several structural determinants are responsible for the antimicrobial and cytolytic activity of antimicrobial peptides. In our study, a new synthetic peptide analog, AamAP1-Lysine from the naturally occurring scorpion venom antimicrobial peptide AamAP1, was designed by modifying the parent peptide in order to increase the positive charge and optimize other physico-chemical parameters involved in antimicrobial activity. AamAP1-Lysine displayed potent antibacterial activity against Gram-positive and Gram-negative bacteria. The minimum inhibitory concentration was in the range of 5 to 15 µM with a 10 fold increase in potency over the parent peptide. The hemolytic and antiproliferative activity of AamAP1-Lysine against eukaryotic mammalian cells was minimal at the concentration range needed to inhibit bacterial growth. The antibacterial mechanism analysis indicated that AamAP1-Lysine is probably inducing bacterial cell death through membrane damage and permeabilization determined by the release of β-galactosidase enzyme from peptide treated E. coli cells. DNA binding studies revealed that AamAP1-Lysine caused complete retardation of DNA migration and could display intracellular activities in addition to the membrane permeabilization mode of action reported earlier. In conclusion, AamAP1-Lysine could prove to be a potential candidate for antimicrobial drug development in future studies.

  17. 无线网络钓鱼 AP 攻击检测技术研究%RESEARCH ON DETECTION TECHNOLOGY OF FISHING AP ATTACK IN WIRELESS NETWORK

    Institute of Scientific and Technical Information of China (English)

    金双齐; 凌捷

    2016-01-01

    With the popularisation of WLAN,the attacks against wireless network are increasingly growing.Wireless fishing AP attack, through passive or active way,induces users to connect fishing AP,and then catches users’sensitive information,it is currently one of the abused attack modes.In light of this,we proposed an improved fishing AP attack detection method,by using diminishing variation of TTL value as well as comprehensively analysing the gateway and routing information,it realises validity detection on AP.Experimental results showed that this method can effectively detect the attacks including wireless fishing AP and wireless man-in-the-middle.%随着无线局域网应用的普及,针对无线网络的攻击方式也逐渐增多。无线钓鱼 AP 攻击通过被动或主动方式诱使用户连接钓鱼 AP,进而获取用户的敏感信息,是当前被滥用的攻击方式之一。针对这种情况,提出一种改进的钓鱼 AP 攻击检测方法,通过利用 TTL 值的递减变化,以及综合分析网关与路由信息,实现对 AP 的合法性检测。实验结果表明,该方法能够有效地检测无线钓鱼 AP 和无线中间人等攻击。

  18. The interaction between the adaptor protein APS and Enigma is involved in actin organisation

    DEFF Research Database (Denmark)

    Barres, Romain; Gonzalez, Teresa; Le Marchand-Brustel, Yannick

    2005-01-01

    and APS were partially co-localised with F-actin in small ruffling structures. Insulin increased the complex formation between APS and Enigma and their co-localisation in large F-actin containing ruffles. While in NIH-3T3 and HeLa cells the co-expression of both Enigma and APS did not modify the actin...... cytoskeleton organisation, expression of Enigma alone led to the formation of F-actin clusters. Similar alteration in actin cytoskeleton organisation was observed in cells expressing both Enigma and APS with a mutation in the NPTY motif. These results identify Enigma as a novel APS-binding protein and suggest...... that the APS/Enigma complex plays a critical role in actin cytoskeleton organisation....

  19. Study on the thermal decomposition behavior of RDX/AP/HTPB propellant%RDX/AP/HTPB推进剂热分解特性研究

    Institute of Scientific and Technical Information of China (English)

    陈沛; 赵凤起; 阴翠梅

    2002-01-01

    利用高压差示扫描量热仪(PDSC)研究了RDX/AP/HTPB推进剂系列配方的热分解性能,发现配方组分的改变对RDX/AP/HTPB推进剂的热分解性能有影响,突出表现在RDX/AP/HTPB推进剂中RDX分解峰变宽,AP放热分解效应增强.推进剂中添加Al粉后,RDX的分解受到抑制,而AP的分解却得到增强.

  20. Biological Oceanography

    Science.gov (United States)

    Dyhrman, Sonya

    2004-10-01

    The ocean is arguably the largest habitat on the planet, and it houses an astounding array of life, from microbes to whales. As a testament to this diversity and its importance, the discipline of biological oceanography spans studies of all levels of biological organization, from that of single genes, to organisms, to their population dynamics. Biological oceanography also includes studies on how organisms interact with, and contribute to, essential global processes. Students of biological oceanography are often as comfortable looking at satellite images as they are electron micrographs. This diversity of perspective begins the textbook Biological Oceanography, with cover graphics including a Coastal Zone Color Scanner image representing chlorophyll concentration, an electron micrograph of a dinoflagellate, and a photograph of a copepod. These images instantly capture the reader's attention and illustrate some of the different scales on which budding oceanographers are required to think. Having taught a core graduate course in biological oceanography for many years, Charlie Miller has used his lecture notes as the genesis for this book. The text covers the subject of biological oceanography in a manner that is targeted to introductory graduate students, but it would also be appropriate for advanced undergraduates.

  1. Quantum Biology

    Directory of Open Access Journals (Sweden)

    Alessandro Sergi

    2009-06-01

    Full Text Available A critical assessment of the recent developmentsof molecular biology is presented.The thesis that they do not lead to a conceptualunderstanding of life and biological systems is defended.Maturana and Varela's concept of autopoiesis is briefly sketchedand its logical circularity avoided by postulatingthe existence of underlying living processes,entailing amplification from the microscopic to the macroscopic scale,with increasing complexity in the passage from one scale to the other.Following such a line of thought, the currently accepted model of condensed matter, which is based on electrostatics and short-ranged forces,is criticized. It is suggested that the correct interpretationof quantum dispersion forces (van der Waals, hydrogen bonding, and so onas quantum coherence effects hints at the necessity of includinglong-ranged forces (or mechanisms for them incondensed matter theories of biological processes.Some quantum effects in biology are reviewedand quantum mechanics is acknowledged as conceptually important to biology since withoutit most (if not all of the biological structuresand signalling processes would not even exist. Moreover, it is suggested that long-rangequantum coherent dynamics, including electron polarization,may be invoked to explain signal amplificationprocess in biological systems in general.

  2. On the evolution of the magnetic field of Ap star $\\alpha^2$ CVn

    CERN Document Server

    Bychkov, V D; Madej, J; Topilskaya, G P

    2016-01-01

    New high-precision measurements of the longitudinal magnetic field of Ap stars suggest the existence of secular intrinsic variations of the global magnetic field in some stars. We argue that such changes are apparent in the Ap star $\\alpha^2$ CVn in the time scale of $\\sim$ 10 years, which results from the analysis of literature data. Therefore, such an observation implies, that the rate of magnetic field evolution of Ap stars is much higher than was previously thought.

  3. HPV16 E6 and E6AP differentially cooperate to stimulate or augment Wnt signaling

    Energy Technology Data Exchange (ETDEWEB)

    Sominsky, Sophia, E-mail: sophia.tab@gmail.com [Department of Clinical Microbiology and Immunology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978 (Israel); Kuslansky, Yael, E-mail: ykuslansky@gmail.com [Department of Clinical Microbiology and Immunology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978 (Israel); Shapiro, Beny, E-mail: benyshap@gmail.com [Department of Clinical Microbiology and Immunology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978 (Israel); Jackman, Anna, E-mail: jackman@post.tau.ac.il [Department of Clinical Microbiology and Immunology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978 (Israel); Haupt, Ygal, E-mail: ygal.haupt@petermac.org [Research Division, The Peter MacCallum Cancer Centre, East Melbourne (Australia); Rosin-Arbesfeld, Rina, E-mail: arina@post.tau.ac.il [Department of Clinical Microbiology and Immunology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978 (Israel); Sherman, Levana, E-mail: lsherman@post.tau.ac.il [Department of Clinical Microbiology and Immunology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978 (Israel)

    2014-11-15

    The present study investigated the roles of E6 and E6AP in the Wnt pathway. We showed that E6 levels are markedly reduced in cells in which Wnt signaling is activated. Coexpression of wild-type or mutant E6AP (C820A) in Wnt-activated cells stabilized E6 and enhanced Wnt/β-catenin/TCF transcription. Expression of E6AP alone in nonstimulated cells elevated β-catenin level, promoted its nuclear accumulation, and activated β-catenin/TCF transcription. A knockdown of E6AP lowered β-catenin levels. Coexpression with E6 intensified the activities of E6AP. Further experiments proved that E6AP/E6 stabilize β-catenin by protecting it from proteasomal degradation. This function was dependent on the catalytic activity of E6AP, the kinase activity of GSK3β and the susceptibility of β-catenin to GSK3β phosphorylation. Thus, this study identified E6AP as a novel regulator of the Wnt signaling pathway, capable of cooperating with E6 in stimulating or augmenting Wnt/β-catenin signaling, thereby possibly contributing to HPV carcinogenesis. - Highlights: • The roles of E6 and E6AP in the Wnt pathway were investigated. • E6AP stabilizes E6 and enhances E6 activity in augmentation of Wnt signaling. • E6AP cooperates with E6 to stabilize β-catenin and stimulate Wnt/β-catenin signaling. • E6AP and E6 act through different mechanisms to augment or stimulate Wnt signaling.

  4. Foldit Biology

    Science.gov (United States)

    2015-07-31

    Report 8/1/2013-7/31/2015 4. TITLE AND SUBTITLE Sa. CONTRACT NUMBER Foldit Biology NOOO 14-13-C-0221 Sb. GRANT NUMBER N/A Sc. PROGRAM ELEMENT...Include area code) Unclassified Unclassified Unclassified (206) 616-2660 Zoran Popović Foldit Biology (Task 1, 2, 3, 4) Final Report...Period Covered by the Report August 1, 2013 – July 31, 2015 Date of Report: July 31, 2015 Project Title: Foldit Biology Contract Number: N00014-13

  5. LBB在AP1000技术中的应用%The Application of LBB in AP1000 Technology

    Institute of Scientific and Technical Information of China (English)

    蒋冬梅; 杜颖; 袁小兰

    2015-01-01

    压水堆核电厂的设计基准事故定义为主管道双端断裂,这一假设过于保守.管道的失效方式往往是先出现破口而后再泄漏,即所谓的先漏后破( Leak Before Break,简称LBB) ,而不是双端断裂. LBB准则是防止核电厂压力管道发生灾难性破裂事故的重要评定准则.本文介绍了LBB评定技术在国外的发展情况和意义,阐述了LBB在AP1000核电厂中的应用情况及LBB评价的理论基础.%Design basis accident of pressurized water reactor nuclear power plant was de-fined as main pipeline double-end fracture, this assumption is too conservative. Pipeline failure modes often appear as first Break then Leak,that is so-called the Leak Before Break ( Leak Before Break,LBB) ,rather than double end fracture. LBB is an important evalua-tion criteria of preventing pressure piping catastrophic rupture accident of nuclear power plant . This paper briefly introduces the development situation and the significance of the o-verseas LBB assessment technology and expounds the application of LBB in AP1000 nucle-ar power plant and the theoretical basis of LBB assessment.

  6. K-AP: Generating specified K clusters by efficient Affinity Propagation

    KAUST Repository

    Zhang, Xiangliang

    2010-12-01

    The Affinity Propagation (AP) clustering algorithm proposed by Frey and Dueck (2007) provides an understandable, nearly optimal summary of a data set. However, it suffers two major shortcomings: i) the number of clusters is vague with the user-defined parameter called self-confidence, and ii) the quadratic computational complexity. When aiming at a given number of clusters due to prior knowledge, AP has to be launched many times until an appropriate setting of self-confidence is found. The re-launched AP increases the computational cost by one order of magnitude. In this paper, we propose an algorithm, called K-AP, to exploit the immediate results of K clusters by introducing a constraint in the process of message passing. Through theoretical analysis and experimental validation, K-AP was shown to be able to directly generate K clusters as user defined, with a negligible increase of computational cost compared to AP. In the meanwhile, K-AP preserves the clustering quality as AP in terms of the distortion. K-AP is more effective than k-medoids w.r.t. the distortion minimization and higher clustering purity. © 2010 IEEE.

  7. Isolation, classification and transcription profiles of the AP2/ERF transcription factor superfamily in citrus.

    Science.gov (United States)

    Xie, Xiu-lan; Shen, Shu-ling; Yin, Xue-ren; Xu, Qian; Sun, Chong-de; Grierson, Donald; Ferguson, Ian; Chen, Kun-song

    2014-07-01

    The AP2/ERF gene family encodes plant-specific transcription factors. In model plants, AP2/ERF genes have been shown to be expressed in response to developmental and environmental stimuli, and many function downstream of the ethylene, biotic, and abiotic stress signaling pathways. In citrus, ethylene is effective in regulation citrus fruit quality, such as degreening and aroma. However, information about the citrus AP2/ERF family is limited, and would enhance our understanding of fruit responses to environmental stress, fruit development and quality. CitAP2/ERF genes were isolated using the citrus genome database, and their expression patterns analyzed by real-time PCR using various orange organs and samples from a fruit developmental series. 126 sequences with homologies to AP2/ERF proteins were identified from the citrus genome, and, on the basis of their structure and sequence, assigned to the ERF family (102), AP2 family (18), RAV family (4) and Soloist (2). MEME motif analysis predicted the defining AP2/ERF domain and EAR repressor domains. Analysis of transcript accumulation in Citrus sinensis cv. 'Newhall' indicated that CitAP2/ERF genes show organ-specific and temporal expression, and provided a framework for understanding the transcriptional regulatory roles of AP2/ERF gene family members in citrus. Hierarchical cluster analysis and t tests identified regulators that potentially function during orange fruit growth and development.

  8. Differential role of SH2-B and APS in regulating energy and glucose homeostasis.

    Science.gov (United States)

    Li, Minghua; Ren, Decheng; Iseki, Masanori; Takaki, Satoshi; Rui, Liangyou

    2006-05-01

    SH2-B and APS, two members of a pleckstrin homology and SH2 domain-containing adaptor family, promote both insulin and leptin signaling in a similar fashion in cultured cells. In addition, APS mediates insulin-stimulated activation of the c-Cbl/CAP/TC10 pathway in cultured adipocytes. Here we characterized genetically modified mice lacking SH2-B, APS, or both to determine the physiological roles of these two proteins in animals. Disruption of the SH2-B gene resulted in obesity, hyperglycemia, hyperinsulinemia, and glucose intolerance. Conversely, deletion of the APS gene did not alter adiposity, energy balance, and glucose metabolism. Energy intake, energy expenditure, fat content, body weight, and plasma insulin, leptin, glucose, and lipid levels were similar between APS(-/-) and WT littermates fed either normal chow or a high-fat diet. Moreover, deletion of APS failed to alter insulin and glucose tolerance. APS(-/-)/SH2-B(-/-) double knockout mice also developed energy imbalance, obesity, hyperleptinemia, hyperinsulinemia, hyperglycemia, and glucose intolerance; however, plasma leptin and insulin levels were significantly lower in APS(-/-)/SH2-B(-/-) than in SH2-B(-/-) mice. These results suggest that SH2-B, but not APS, is a key positive regulator of energy and glucose metabolism in mice.

  9. Heterologous expression in Tritrichomonas foetus of functional Trichomonas vaginalis AP65 adhesin

    Science.gov (United States)

    Kucknoor, Ashwini S; Mundodi, Vasanthakrishna; Alderete, JF

    2005-01-01

    Background Trichomonosis, caused by Trichomonas vaginalis, is the number one, nonviral sexually transmitted infection that has adverse consequences for the health of women and children. The interaction of T. vaginalis with vaginal epithelial cells (VECs), a step preparatory to infection, is mediated in part by the prominent surface protein AP65. The bovine trichomonad, Tritrichomonas foetus, adheres poorly to human VECs. Thus, we established a transfection system for heterologous expression of the T. vaginalis AP65 in T. foetus, as an alternative approach to confirm adhesin function for this virulence factor. Results In this study, we show stable transfection and expression of the T. vaginalis ap65 gene in T. foetus from an episomal pBS-ap65-neo plasmid. Expression of the gene and protein was confirmed by RT-PCR and immunoblots, respectively. AP65 in transformed T. foetus bound to host cells. Specific mAbs revealed episomally-expressed AP65 targeted to the parasite surface and hydrogenosome organelles. Importantly, surface-expression of AP65 in T. foetus paralleled increased levels of adherence of transfected bovine trichomonads to human VECs. Conclusion The T. vaginalis AP65 adhesin was stably expressed in T. foetus, and the data obtained using this heterologous system strongly supports the role of AP65 as a prominent adhesin for T. vaginalis. In addition, the heterologous expression in T. foetus of a T. vaginalis gene offers an important, new approach for confirming and characterizing virulence factors. PMID:15748280

  10. Heterologous expression in Tritrichomonas foetus of functional Trichomonas vaginalis AP65 adhesin

    Directory of Open Access Journals (Sweden)

    Alderete JF

    2005-03-01

    Full Text Available Abstract Background Trichomonosis, caused by Trichomonas vaginalis, is the number one, nonviral sexually transmitted infection that has adverse consequences for the health of women and children. The interaction of T. vaginalis with vaginal epithelial cells (VECs, a step preparatory to infection, is mediated in part by the prominent surface protein AP65. The bovine trichomonad, Tritrichomonas foetus, adheres poorly to human VECs. Thus, we established a transfection system for heterologous expression of the T. vaginalis AP65 in T. foetus, as an alternative approach to confirm adhesin function for this virulence factor. Results In this study, we show stable transfection and expression of the T. vaginalis ap65 gene in T. foetus from an episomal pBS-ap65-neo plasmid. Expression of the gene and protein was confirmed by RT-PCR and immunoblots, respectively. AP65 in transformed T. foetus bound to host cells. Specific mAbs revealed episomally-expressed AP65 targeted to the parasite surface and hydrogenosome organelles. Importantly, surface-expression of AP65 in T. foetus paralleled increased levels of adherence of transfected bovine trichomonads to human VECs. Conclusion The T. vaginalis AP65 adhesin was stably expressed in T. foetus, and the data obtained using this heterologous system strongly supports the role of AP65 as a prominent adhesin for T. vaginalis. In addition, the heterologous expression in T. foetus of a T. vaginalis gene offers an important, new approach for confirming and characterizing virulence factors.

  11. Obesity-linked homologues TfAP-2 and Twz establish meal frequency in Drosophila melanogaster.

    Directory of Open Access Journals (Sweden)

    Michael J Williams

    2014-09-01

    Full Text Available In all animals managing the size of individual meals and frequency of feeding is crucial for metabolic homeostasis. In the current study we demonstrate that the noradrenalin analogue octopamine and the cholecystokinin (CCK homologue Drosulfakinin (Dsk function downstream of TfAP-2 and Tiwaz (Twz to control the number of meals in adult flies. Loss of TfAP-2 or Twz in octopaminergic neurons increased the size of individual meals, while overexpression of TfAP-2 significantly decreased meal size and increased feeding frequency. Of note, our study reveals that TfAP-2 and Twz regulate octopamine signaling to initiate feeding; then octopamine, in a negative feedback loop, induces expression of Dsk to inhibit consummatory behavior. Intriguingly, we found that the mouse TfAP-2 and Twz homologues, AP-2β and Kctd15, co-localize in areas of the brain known to regulate feeding behavior and reward, and a proximity ligation assay (PLA demonstrated that AP-2β and Kctd15 interact directly in a mouse hypothalamus-derived cell line. Finally, we show that in this mouse hypothalamic cell line AP-2β and Kctd15 directly interact with Ube2i, a mouse sumoylation enzyme, and that AP-2β may itself be sumoylated. Our study reveals how two obesity-linked homologues regulate metabolic homeostasis by modulating consummatory behavior.

  12. APS-U Definitions of Signs and Conventions Related to Magnets

    Energy Technology Data Exchange (ETDEWEB)

    Doose, Charles [Argonne National Lab. (ANL), Argonne, IL (United States). Accelerator Systems Division; Jain, Animesh [Brookhaven National Lab. (BNL), Upton, NY (United States). Superconducting Magnet Division

    2014-08-21

    The APS-U is planned to be a 4th generation hard X-ray light source utilizing a multi-bend achromat (MBA) magnet lattice. The MBA lattice will be installed in the existing APS storage ring enclosure. The stored electron beam will circulate clockwise when viewed from above. The X-ray beamlines will for the most part exit at the same source points as the present APS. This document defines the signs and conventions related to the APS-U MBA magnets. Included in this document are: the local magnet coordinate system, definitions of mechanical and magnetic centers, definitions of multipole field errors, magnetic roll angle, and magnet polarities.

  13. Plant resistance against the parasitic nematode Heterodera schachtii is mediated by MPK3 and MPK6 kinases, which are controlled by the MAPK phosphatase AP2C1 in Arabidopsis.

    Science.gov (United States)

    Sidonskaya, Ekaterina; Schweighofer, Alois; Shubchynskyy, Volodymyr; Kammerhofer, Nina; Hofmann, Julia; Wieczorek, Krzysztof; Meskiene, Irute

    2016-01-01

    Plant-parasitic cyst nematodes infect plants and form highly sophisticated feeding sites in roots. It is not known which plant cell signalling mechanisms trigger plant defence during the early stages of nematode parasitism. Mitogen-activated protein kinases (MAPKs) are central components of protein phosphorylation cascades transducing extracellular signals to plant defence responses. MAPK phosphatases control kinase activities and the signalling outcome. The involvement and the role of MPK3 and MPK6, as well as the MAPK phosphatase AP2C1, is demonstrated during parasitism of the beet cyst nematode Heterodera schachtii in Arabidopsis. Our data reveal notable activation patterns of plant MAPKs and the induction of AP2C1 suggesting the attenuation of defence signalling in plant cells during early nematode infection. It is demonstrated that the ap2c1 mutant that is lacking AP2C1 is more attractive but less susceptible to nematodes compared with the AP2C1-overexpressing line. This implies that the function of AP2C1 is a negative regulator of nematode-induced defence. By contrast, the enhanced susceptibility of mpk3 and mpk6 plants indicates a positive role of stress-activated MAPKs in plant immunity against nematodes. Evidence is provided that phosphatase AP2C1, as well as AP2C1-targeted MPK3 and MPK6, are important regulators of plant-nematode interaction, where the co-ordinated action of these signalling components ensures the timely activation of plant defence. © The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology.

  14. Biology teachers

    African Journals Online (AJOL)

    Mathematics, Science and Biology teachers code switch when they teach. ... (by constantly translating back and forth), and argue for a 'separation approach' ..... for the classroom, only 3 students did not give an answer to this open-ended.

  15. Analysis of human reliability in the APS of fire. Application of NUREG-1921; Analisis de Fiabilidad Humana en el APS de Incendios. Aplicacion del NUREG-1921

    Energy Technology Data Exchange (ETDEWEB)

    Perez Torres, J. L.; Celaya Meler, M. A.

    2014-07-01

    An analysis of human reliability in a probabilistic safety analysis (APS) of fire aims to identify, describe, analyze and quantify, in a manner traceable, human actions that can affect the mitigation of an initiating event produced by a fire. (Author)

  16. Preface: 18th Aps-Sccm and 24th Airapt

    Science.gov (United States)

    Collins, Gilbert; Moore, David S.; Yoo, Choong-Shik; Buttler, William; Furlanetto, Michael; Evans, William

    2014-05-01

    The 18th Biennial International Conference of the APS Topical Group on Shock Compression of Condensed Matter in conjunction with the 24th Biennial International Conference of the International Association for the Advancement of High Pressure Science & Technology (AIRAPT) was held at the Westin Hotel in Seattle, Washington from 7-12 July, 2013. This is only the second time that these two organizations have held a Joint Conference — the first was 20 years previous (1993) in Colorado Springs, Colorado. Seattle was chosen for this joint conference because of its central location for the world-wide attendees as well as its metropolitan vibrancy. The scientific program consisted of 858 scheduled presentations organized into 23 topical areas and included contributed (537), invited (95), and plenary (6) lectures, as well as two poster sessions with 110 posters each. The scientific focus of the Joint Conference was on fundamental and applied research topics related to the static or dynamic compression of condensed matter. This multidisciplinary field of research encompasses areas of physics, chemistry, materials science, mechanics, geophysics and planetary physics, and applied mathematics. Experimental, computational and theoretical studies all play important roles. The organizers endeavored to intertwine static and dynamic experimental alongside computational and theoretical studies of similar materials in the organization of the sessions. This goal was aided by the addition of three special focus sessions on deep carbon budget, high energy density materials, and dynamic response of materials. 722 scientists and engineers from 25 countries registered at the conference, including 132 students from 12 countries. The attendee countries represented included: Argentina (2), Australia (2), Brazil (3), Canada (25), China (22), Czech Republic (2), France (35), Germany (19), India (6), Israel (21), Italy (10), Japan (49), Netherlands (1), Poland (1), Portugal (2), Russia (26

  17. In vitro characterization of hydroxyapatite layers deposited by APS and HVOF thermal spraying methods

    Directory of Open Access Journals (Sweden)

    Radu Alexandru Roşu

    2012-03-01

    Full Text Available Titanium alloys are successfully used in medicine as implants due to their high mechanical properties and good biocompatibility. To improve implant osseointegration of titanium alloys, they are covered with hydroxyapatite because of its bioactive properties. Coating the implants with hydroxyapatite by thermal spraying, due to the temperatures developed during the deposition process, the structure can be degraded, leading to formation of secondary phases, such as TCP, TT CP, CaO. The paper presents the experimental results of hydroxyapatite layers deposition by two thermal spraying methods: Atmospheric Plasma Spraying (APS and High Velocity Oxy-Fuel (HVOF. The microstructure of the deposited layers is characterized by X-ray diffraction analysis and electronic microscopy. The bioactivity of the hydroxyapatite layers was investigated in Simulated Body Fluid (SBF by immersing the covered samples deposited by the two thermal spraying methods. In both cases the coatings did not present defects as cracks or microcracks. X-ray diffraction performed on hydroxyapatite deposited layers shows that the structure was strongly influenced by plasma jet temperature, the structure consisting mainly of TCP (Ca3PO42. The samples deposited by HVO F after immersing in SBF lead to formation of biological hydroxyapatite, certifying the good bioactivity of the coatings.

  18. Radioactive air emissions notice of construction for installation and operation of a waste retrieval system and tanks 241-AP-102 and 241-AP-104 project

    Energy Technology Data Exchange (ETDEWEB)

    DEXTER, M.L.

    1999-11-15

    This document serves as a notice of construction (NOC) pursuant to the requirements of Washington Administrative Code (WAC) 246 247-060, and as a request for approval to modify pursuant to 40 Code of Federal Regulations (CFR) 61 07 for the installation and operation of one waste retrieval system in the 24 1 AP-102 Tank and one waste retrieval system in the 241 AP 104 Tank Pursuant to 40 CFR 61 09 (a)( 1) this application is also intended to provide anticipated initial start up notification Its is requested that EPA approval of this application will also constitute EPA acceptance of the initial start up notification Project W 211 Initial Tank Retrieval Systems (ITRS) is scoped to install a waste retrieval system in the following double-shell tanks 241-AP 102-AP 104 AN 102, AN 103, AN-104, AN 105, AY 102 AZ 102 and SY-102 between now and the year 2011. Because of the extended installation schedules and unknowns about specific activities/designs at each tank, it was decided to submit NOCs as that information became available This NOC covers the installation and operation of a waste retrieval system in tanks 241 AP-102 and 241 AP 104 Generally this includes removal of existing equipment installation of new equipment and construction of new ancillary equipment and buildings Tanks 241 AP 102 and 241 AP 104 will provide waste feed for immobilization into a low activity waste (LAW) product (i.e. glass logs) The total effective dose equivalent (TEDE) to the offsite maximally exposed individual (MEI) from the construction activities is 0 045 millirem per year The unabated TEDE to the offsite ME1 from operation of the mixer pumps is 0 042 millirem per year.

  19. ANALISIS PEMISAHAN UAP KERING PADA SEPARATOR PEMBANGKIT UAP AP1000

    Directory of Open Access Journals (Sweden)

    Sukmanto Dibyo

    2015-03-01

    Full Text Available Pembangkit uap merupakan penukar panas, yang bagian atasnya adalah steam drum yang memuat separator uap. Separator ini memisahkan kandungan air yang terbawa oleh uap dari sisi shell. Uap kering yang dihasilkan dari separator digunakan untuk memutar turbin. Tujuan penelitian ini adalah menganalisis karakteristik pemisahan uap kering pada separator pembangkit uap AP1000, berdasarkan parameter fraksi void dalam kondisi aliran dua fasa dengan menggunakan RELAP5/SCDAP.Mod3.4. Data awal mencakup data dimensi dan parameter temperatur, tekanan, laju alir massa (943,0 kg/s, fraksi void, entalpi cair dan uap saturasi. Untuk memperoleh karakteristik separator maka analisis ini difokuskan pada sistem separator secara terpisah pada kondisi tunak. Nodalisasi untuk RELAP5/SCDAP.Mod3.4, terdiri dari model separator (default, volume, junction dan time-dependent junction dengan time-dependent volume sebagai kondisi batas. Kondisi uap kering terlihat pada parameter fraksi void yang keluar dari separator. Hasil menunjukkan bahwa semakin tinggi temperatur uap dari sisi shell pembangkit uap maka cenderung semakin tinggi uap kering yang dihasilkan. Pemisahan uap kering yang keluar dari separator ditunjukkan pada parameter fraksi void sebesar sekitar 0,99. Kata kunci: separator uap, pembangkit uap, RELAP5/SCDAP.Mod3.4, fraksi void A steam generator is heat exchanger in which the top side is steam drum containing steam separator. The separator is used to separate the water content carried by the steam come from The shell side. Dry vapor produced from the separator is used to turn turbines. this paper analyzes the dry Steam separation characteristic In The separator of AP1000 steam generator based on the parameters of void fraction in two phase flow using RELAP5/SCDAP.Mod3.4. The initial data include data dimensions and operating data (temperature, pressure, flow rate (943.0 KG/S, void fraction, enthalpy of water and saturation vapor. To obtain the characteristics of

  20. Magnetic AP Stars in the Hertzsprung-Russell Diagram

    Science.gov (United States)

    Hubrig, S.; North, P.; Mathys, G.

    2000-08-01

    The evolutionary state of magnetic Ap stars is rediscussed using the recently released Hipparcos data. The distribution of the magnetic Ap stars of mass below 3 Msolar in the H-R diagram differs from that of the normal stars in the same temperature range at a high level of significance. Magnetic stars are concentrated toward the center of the main-sequence band. This is shown in two forms of the H-R diagram: one where logL is plotted against logTeff and a version more directly tied to the observed quantities, showing the astrometry-based luminosity (Arenou & Luri) against the (B2-G)0 index of Geneva photometry. In particular, it is found that magnetic fields appear only in stars that have already completed at least approximately 30% of their main-sequence lifetime. No clear picture emerges as to the possible evolution of the magnetic field across the main sequence. Hints of some (loose) relations between magnetic field strength and other stellar parameters are found: stars with shorter periods tend to have stronger fields, as do higher temperature and higher mass stars. A marginal trend of the magnetic flux to be lower in more slowly rotating stars may possibly be seen as suggesting a dynamo origin for the field. No correlation between the rotation period and the fraction of the main-sequence lifetime completed is observed, indicating that the slow rotation in these stars must already have been achieved before they became observably magnetic. Based on data from the ESA Hipparcos satellite and on observations collected at the European Southern Observatory (La Silla, Chile; ESO programs Nos. 43.7-004, 44.7-012, 49.7-030, 50.7-067, 51.7-041, 52.7-063, 53.7-028, 54.E-0416, and 55.E-0751), at the Observatoire de Haute-Provence (Saint-Michel l'Observatoire, France), at Kitt Peak National Observatory, and at the Canada-France-Hawaii Telescope.

  1. AP-2δ is a crucial transcriptional regulator of the posterior midbrain.

    Directory of Open Access Journals (Sweden)

    Katrin Hesse

    Full Text Available Ap-2 transcription factors comprise a family of 5 closely related sequence-specific DNA binding proteins that play pivotal and non-redundant roles in embryonic organogenesis. To investigate the function of Ap-2δ, wδe analyzed its expression during embryogenesis and generated Ap-2δ-deficient mice. In line with the specific expression pattern of Ap-2δ in the mesencephalic tectum and the dorsal midbrain, Ap-2δ-deficient mice failed to maintain the colliculus inferior, a derivative of the dorsal midbrain, as a consequence of increased apoptotic cell death. To identify specific Ap-2δ target genes in cells of the developing dorsal midbrain, we performed whole genome analysis of cDNA expression levels. This approach identified a set of 12 putative target genes being expressed in the developing midbrain, including the transcription factors Pitx2, Mef2c, Bhlhb4 and Pou4f3. Using chromatin immunoprecipitation (CHIP we showed that some of these genes are direct targets of Ap-2δ. Consistently, we demonstrate that Ap-2δ occupies and activates the Pou4f3 and Bhlhb4 promoters. In addition, known Pou4f3 target genes were downregulated in the posterior midbrain of Ap-2δ-deficient mice. Despite the absence of a central part of the auditory pathway, the presence of neuronal responses to sounds in the neocortex of Ap-2δ-deficient mice indicates that auditory information from the brainstem still reaches the neocortex. In summary, our data define Ap-2δ as an important transcription factor, specifying gene expression patterns required for the development of the posterior midbrain.

  2. Religião, política e ordem social nos Atos Apócrifos dos Apóstolos

    Directory of Open Access Journals (Sweden)

    José Adriano Filho

    2016-01-01

    Full Text Available Os Atos Apócrifos dos Apóstolos recontam a tradição dos apóstolos e suas façanhas públicas. Os apóstolos são apresentados em constante conflito com o mundo à sua volta. Eles demonstram o poder do único Deus verdadeiro por meio de milagres, ascetismo e martírio. O cristianismo que eles representam contrasta com as crenças e poder do mundo greco-romano. Esta instância antissocial é reforçada pela mensagem de castidade anunciada pelos apóstolos, a qual interrompe o casamento, e nos conflitos com as autoridades civis e religiosas. Eles têm preocupações apologéticas, missionárias e de edificação, mas rejeitam os ideais culturais e sociais tradicionais em favor de novos ideais. Os Atos Apócrifos também refletem a rede do patronato social e político contemporâneo do Império romano. Eles redefinem o patronato de uma perspectiva cristã e desafiam o sistema estabelecido ao apresentar a Cristo como o verdadeiro patrono, superior aos patronos humanos, incluindo o imperador. A tentativa de reformular as pressuposições estabelecidas é uma característica que define a identidade cristã representada nesta literatura.

  3. TinyCoAP: A Novel Constrained Application Protocol (CoAP Implementation for Embedding RESTful Web Services in Wireless Sensor Networks Based on TinyOS

    Directory of Open Access Journals (Sweden)

    Anna Calveras

    2013-05-01

    Full Text Available In this paper we present the design and implementation of the Constrained Application Protocol (CoAP for TinyOS, which we refer to as TinyCoAP. CoAP seeks to apply the same application transfer paradigm and basic features of HTTP to constrained networks, while maintaining a simple design and low overhead. The design constraints of Wireless Sensor Networks (WSNs require special attention in the design process of the CoAP implementation. We argue that better performance and minimal resource consumption can be achieved developing a native library for the operating system embedded in the network. TinyOS already includes in its distribution an implementation of CoAP called CoapBlip. However, this is based on a library not originally designed to meet the requirements of TinyOS. We demonstrate the effectiveness of our approach by a comprehensive performance evaluation. In particular, we test and evaluate TinyCoAP and CoapBlip in a real scenario, as well as solutions based on HTTP. The evaluation is performed in terms of latency, memory occupation, and energy consumption. Furthermore, we evaluate the reliability of each solution by measuring the goodput obtained in a channel affected by Rayleigh fading. We also include a study on the effects that high workloads have on a server.

  4. AP1000控制棒棒位的数字化测量技术分析%Technical Analysis of Digital Measurement in AP1000 Control Rods

    Institute of Scientific and Technical Information of China (English)

    彭小强; 郑养波

    2012-01-01

    结合AP1000的数字化棒位指示系统,通过分析AP1000控制棒的组成、控制棒驱动机构和棒位测量探测器布置,并从数字化测量角度指出了非接触式传感器测量、探测线圈的冗余设计和数字化数据传输等可靠性技术的应用,确保了AP1000反应堆棒位测量精度和棒位控制系统的安全可靠.%It analyzes the control rods、RDM and rod position measurement detector layout based on AP1000 digital rod position system. At the same time, the application of high reliability technologies in AP1000, such as non - contact sensor measurements 、 the redundant design of detection coil and digital data transmission, which can ensure the accuracy of rod position measurement and the safety and reliability of control system in AP1000.

  5. New Beam Position Monitor System Design for the APS Injector

    Science.gov (United States)

    Lill, R.; Singh, O.; Arnold, N.

    2002-12-01

    Demands on the APS injector have evolved over the last few years to the point that an upgrade to the existing beam position monitor (BPM) electronics is required. The injector is presently being used as a source for both the low-energy undulator test line (LEUTL) project and the top-up mode of operation. These new requirements and the fact that many new rf receiver components are available at reasonable cost make this upgrade very desirable at this time. The receiver topology selected is a logarithmic processor, which is designed around the Analog Devices AD8313 log amplifier demodulation chip. This receiver will become the universal replacement for all injector applications measuring positions signals from 352 to 2856 MHz with minimum changes in hardware and without the use of a downconverter. The receiver design features integrated front-end gain and built-in self test. The data acquisition being considered at this time is a 100-MHz, 12-bit transient recorder digitizer. The latest experimental and commissioning data and results will be presented.

  6. Tandem Differential Mobility Analyzer/Aerodynamic Particle Sizer (APS) Handbook

    Energy Technology Data Exchange (ETDEWEB)

    Collins, D

    2010-06-18

    The tandem differential mobility analyzer (TDMA) is a single instrument that cycles through a series of complementary measurements of the physical properties of size-resolved submicron particles. In 2008, the TDMA was augmented through the addition of an aerodynamic particle sizer (APS), which extends the upper limit of the measured size distribution into the supermicron range. These two instruments are operated in parallel, but because they are controlled by a common computer and because the size distributions measured by the two are integrated in the produced datastreams, they are described together here. Throughout the day, the TDMA sequentially measures submicron aerosol size distributions and size-resolved hygroscopic growth distributions. More specifically, the instrument is operated as a scanning DMA to measure size distributions and as a TDMA to measure size-resolved hygroscopicity. A typical measurement sequence requires roughly 45 minutes. Each morning additional measurements are made of the relative humidity (RH) dependent hygroscopicity and temperature-dependent volatility of size-resolved particles. When the outside temperature and RH are within acceptable ranges, the hydration state of size-resolved particles is also characterized. The measured aerosol distributions complement the array of aerosol instruments in the Aerosol Observing System (AOS) and provide additional details of the light-scattering and cloud-nucleating characteristics of the aerosol.

  7. Catastrophic APS in the context of other thrombotic microangiopathies.

    Science.gov (United States)

    Rodríguez-Pintó, Ignasi; Espinosa, Gerard; Cervera, Ricard

    2015-01-01

    The catastrophic antiphospholipid syndrome (CAPS) is a rare disease that affects 1 % of cases with antiphospholipid syndrome (APS). CAPS can mimic or overlap with different thrombotic diseases; many patients present with a microthrombotic storm or thrombotic microangiopathic hemolytic anemia (TMHA). Thus, the differential diagnosis of CAPS includes thrombotic thrombocytopenic purpura (TTP), typical and atypical hemolytic uremic syndrome (HUS), systemic infections, malignancies, pregnancy-related disorders, malignant hypertension, heparin-induced thrombocytopenia, and drug-induced thrombotic microangiopathies. Antiphospholipid antibody (aPL) positivity has been proposed as the clue in this differential diagnosis; however, aPL can also occur in healthy people and in those with infections or malignancies. Thus, the differential diagnosis of an aPL-positive patient presenting with a microthrombotic storm is broad; the workup should include a special attention to signs of infection and disseminated malignant disease, assessing the funduscopic signs of malignant hypertension, testing ADAMTS13 activity and anti-heparin-platelet factor 4 (HPF4) antibodies, and searching previous exposure to certain drugs. This article aims to review the main diseases included in the differential diagnosis of CAPS in the context of other thrombotic microangiopathies.

  8. Initial commissioning results from the APS loss monitor system

    Science.gov (United States)

    Patterson, Donald R.

    1997-01-01

    The design of the beam loss monitor system for the Argonne National Laboratory Advanced Photon Source is based on using a number of air dielectric coaxial cables as long ionization chambers. Results to date show that the loss monitor is useful in helping to determine the cause of injection losses and losses large enough to limit circulating currents in the storage ring to short lifetimes. Sensitivities ranging from 13 to 240 pC of charge collected in the injector BTS (booster-to-storage-ring) loss monitor per picocoulomb of loss have been measured, depending on the loss location. These results have been used to predict that the storage ring loss monitor leakage current limit of 10 pA per cable should allow detection of losses resulting in beam lifetimes of 100 hours or less with 100 mA stored beam. Significant DC bias levels associated with the presence of stored beam have been observed. These large bias levels are most likely caused by the loss monitor responding to hard x-ray synchrotron radiation. No such response to synchrotron radiation was observed during earlier tests at SSRL. However, the loss monitor response to average stored beam current in APS has provided a reasonable alternative to the DC current transformer (DCCT) for measuring beam lifetimes.

  9. STREAK CAMERA MEASUREMENTS OF THE APS PC GUN DRIVE LASER

    Energy Technology Data Exchange (ETDEWEB)

    Dooling, J. C.; Lumpkin, A. H.

    2017-06-25

    We report recent pulse-duration measurements of the APS PC Gun drive laser at both second harmonic and fourth harmonic wavelengths. The drive laser is a Nd:Glass-based chirped pulsed amplifier (CPA) operating at an IR wavelength of 1053 nm, twice frequency-doubled to obtain UV output for the gun. A Hamamatsu C5680 streak camera and an M5675 synchroscan unit are used for these measurements; the synchroscan unit is tuned to 119 MHz, the 24th subharmonic of the linac s-band operating frequency. Calibration is accomplished both electronically and optically. Electronic calibration utilizes a programmable delay line in the 119 MHz rf path. The optical delay uses an etalon with known spacing between reflecting surfaces and is coated for the visible, SH wavelength. IR pulse duration is monitored with an autocorrelator. Fitting the streak camera image projected profiles with Gaussians, UV rms pulse durations are found to vary from 2.1 ps to 3.5 ps as the IR varies from 2.2 ps to 5.2 ps.

  10. Absolute properties of the eclipsing binary star AP Andromedae

    Energy Technology Data Exchange (ETDEWEB)

    Sandberg Lacy, Claud H. [Physics Department, University of Arkansas, Fayetteville, AR 72701 (United States); Torres, Guillermo [Harvard-Smithsonian Center for Astrophysics, 60 Garden Street, Cambridge, MA 02138 (United States); Fekel, Francis C.; Muterspaugh, Matthew W., E-mail: clacy@uark.edu, E-mail: gtorres@cfa.harvard.edu, E-mail: fekel@evans.tsuniv.edu, E-mail: matthew1@coe.tsuniv.edu [Center of Excellence in Information Systems, Tennessee State University, Nashville, TN 37209 (United States)

    2014-06-01

    AP And is a well-detached F5 eclipsing binary star for which only a very limited amount of information was available before this publication. We have obtained very extensive measurements of the light curve (19,097 differential V magnitude observations) and a radial velocity curve (83 spectroscopic observations) which allow us to fit orbits and determine the absolute properties of the components very accurately: masses of 1.277 ± 0.004 and 1.251 ± 0.004 M {sub ☉}, radii of 1.233 ± 0.006 and 1.1953 ± 0.005 R {sub ☉}, and temperatures of 6565 ± 150 K and 6495 ± 150 K. The distance to the system is about 400 ± 30 pc. Comparison with the theoretical properties of the stellar evolutionary models of the Yonsei-Yale series of Yi et al. shows good agreement between the observations and the theory at an age of about 500 Myr and a slightly sub-solar metallicity.

  11. Scribble1/AP2 Complex Coordinates NMDA Receptor Endocytic Recycling

    Directory of Open Access Journals (Sweden)

    Nicolas H. Piguel

    2014-10-01

    Full Text Available The appropriate trafficking of glutamate receptors to synapses is crucial for basic synaptic function and synaptic plasticity. It is now accepted that NMDA receptors (NMDARs internalize and are recycled at the plasma membrane but also exchange between synaptic and extrasynaptic pools; these NMDAR properties are also key to governing synaptic plasticity. Scribble1 is a large PDZ protein required for synaptogenesis and synaptic plasticity. Herein, we show that the level of Scribble1 is regulated in an activity-dependent manner and that Scribble1 controls the number of NMDARs at the plasma membrane. Notably, Scribble1 prevents GluN2A subunits from undergoing lysosomal trafficking and degradation by increasing their recycling to the plasma membrane following NMDAR activation. Finally, we show that a specific YxxR motif on Scribble1 controls these mechanisms through a direct interaction with AP2. Altogether, our findings define a molecular mechanism to control the levels of synaptic NMDARs via Scribble1 complex signaling.

  12. Coming Soon: CADRE (Career Advancement and Development Resources and Education) website for all APS members

    Science.gov (United States)

    The Council of the American Phytopathological Society (APS) approved an initiative in February 2013 to create a web resource called CADRE (Career Advancement and Development Resources and Education). CADRE is to provide APS members an archive of articles, videos, and webinars about a variety of prof...

  13. Obstetrical APS : Is there a place for hydroxychloroquine to improve the pregnancy outcome?

    NARCIS (Netherlands)

    Mekinian, Arsene; Costedoat-Chalumeau, Nathalie; Masseau, Agathe; Tincani, Angela; De Caroli, Sara; Alijotas-Reig, Jaume; Ruffatti, Amelia; Ambrozic, Ales; Botta, Angela; Le Guern, Véronique; Fritsch-Stork, Ruth; Nicaise-Roland, Pascale; Carbonne, Bruno; Carbillon, Lionel; Fain, Olivier

    2015-01-01

    The use of the conventional APS treatment (the combination of low-dose aspirin and LMWH) dramatically improved the obstetrical prognosis in primary obstetrical APS (OAPS). The persistence of adverse pregnancy outcome raises the need to find other drugs to improve obstetrical outcome. Hydroxychloroqu

  14. Amphioxus and lamprey AP-2 genes: implications for neural crest evolution and migration patterns

    Science.gov (United States)

    Meulemans, Daniel; Bronner-Fraser, Marianne

    2002-01-01

    The neural crest is a uniquely vertebrate cell type present in the most basal vertebrates, but not in cephalochordates. We have studied differences in regulation of the neural crest marker AP-2 across two evolutionary transitions: invertebrate to vertebrate, and agnathan to gnathostome. Isolation and comparison of amphioxus, lamprey and axolotl AP-2 reveals its extensive expansion in the vertebrate dorsal neural tube and pharyngeal arches, implying co-option of AP-2 genes by neural crest cells early in vertebrate evolution. Expression in non-neural ectoderm is a conserved feature in amphioxus and vertebrates, suggesting an ancient role for AP-2 genes in this tissue. There is also common expression in subsets of ventrolateral neurons in the anterior neural tube, consistent with a primitive role in brain development. Comparison of AP-2 expression in axolotl and lamprey suggests an elaboration of cranial neural crest patterning in gnathostomes. However, migration of AP-2-expressing neural crest cells medial to the pharyngeal arch mesoderm appears to be a primitive feature retained in all vertebrates. Because AP-2 has essential roles in cranial neural crest differentiation and proliferation, the co-option of AP-2 by neural crest cells in the vertebrate lineage was a potentially crucial event in vertebrate evolution.

  15. Reiterative AP2a activity controls sequential steps in the neural crest gene regulatory network.

    Science.gov (United States)

    de Crozé, Noémie; Maczkowiak, Frédérique; Monsoro-Burq, Anne H

    2011-01-04

    The neural crest (NC) emerges from combinatorial inductive events occurring within its progenitor domain, the neural border (NB). Several transcription factors act early at the NB, but the initiating molecular events remain elusive. Recent data from basal vertebrates suggest that ap2 might have been critical for NC emergence; however, the role of AP2 factors at the NB remains unclear. We show here that AP2a initiates NB patterning and is sufficient to elicit a NB-like pattern in neuralized ectoderm. In contrast, the other early regulators do not participate in ap2a initiation at the NB, but cooperate to further establish a robust NB pattern. The NC regulatory network uses a multistep cascade of secreted inducers and transcription factors, first at the NB and then within the NC progenitors. Here we report that AP2a acts at two distinct steps of this cascade. As the earliest known NB specifier, AP2a mediates Wnt signals to initiate the NB and activate pax3; as a NC specifier, AP2a regulates further NC development independent of and downstream of NB patterning. Our findings reconcile conflicting observations from various vertebrate organisms. AP2a provides a paradigm for the reiterated use of multifunctional molecules, thereby facilitating emergence of the NC in vertebrates.

  16. The History of the APS Shock Compression of Condensed Matter Topical Group

    Energy Technology Data Exchange (ETDEWEB)

    Forbes, J W

    2001-05-02

    In order to provide broader scientific recognition and to advance the science of shock compressed condensed matter, a group of American Physical Society (APS) members worked within the Society to make this field an active part of the APS. Individual papers were presented at APS meetings starting in the 1940's and shock wave sessions were organized starting with the 1967 Pasadena meeting. Shock wave topical conferences began in 1979 in Pullman, WA. Signatures were obtained on a petition in 1984 from a balanced cross-section of the shock wave community to form an APS Topical Group (TG). The APS Council officially accepted the formation of the Shock Compression of Condensed Matter (SCCM) TG at its October 1984 meeting. This action firmly aligned the shock wave field with a major physical science organization. Most early topical conferences were sanctioned by the APS while those held after 1992 were official APS meetings. The topical group organizes a shock wave topical conference in odd numbered years while participating in shock wave/high pressure sessions at APS general meetings in even numbered years.

  17. History of the APS Topical Group on Shock Compression of Condensed Matter

    Energy Technology Data Exchange (ETDEWEB)

    Forbes, J W

    2001-10-19

    In order to provide broader scientific recognition and to advance the science of shock compressed condensed matter, a group of American Physical Society (APS) members worked within the Society to make this field an active part of the APS. Individual papers were presented at APS meetings starting in the 1940's and shock wave sessions were organized starting with the 1967 Pasadena meeting. Shock wave topical conferences began in 1979 in Pullman, WA. Signatures were obtained on a petition in 1984 from a balanced cross-section of the shock wave community to form an APS Topical Group (TG). The APS Council officially accepted the formation of the Shock Compression of Condensed Matter (SCCM) TG at its October 1984 meeting. This action firmly aligned the shock wave field with a major physical science organization. Most early topical conferences were sanctioned by the APS while those held after 1992 were official APS meetings. The topical group organizes a shock wave topical conference in odd numbered years while participating in shock wavehigh pressure sessions at APS general meetings in even numbered years.

  18. Understanding and Using the New Guided-Inquiry AP Chemistry Laboratory Manual

    Science.gov (United States)

    Cacciatore, Kristen L.

    2014-01-01

    To support teaching and learning in the advanced placement (AP) chemistry laboratory, the College Board published a laboratory manual, "AP Chemistry Guided-Inquiry Experiments: Applying the Science Practices," in 2013 as part of the redesigned course. This article provides a discussion of the rationale for the existence of the manual as…

  19. Foundations for College and Beyond: Looking Back on AP Art History

    Science.gov (United States)

    Schoenbohm, Laurel

    2013-01-01

    It was years after this author's AP Art History course in high school, and two years after college. She and some friends decided to fill a day during the Thanksgiving visits appreciating fine art. Prior to that AP course her senior year of high school, touring an art museum had seemed like the equivalent of going to the dentist. But after…

  20. 75 FR 20774 - Establishment of Class E Airspace; Fort A.P. Hill, VA

    Science.gov (United States)

    2010-04-21

    ... Federal Aviation Administration 14 CFR Part 71 Establishment of Class E Airspace; Fort A.P. Hill, VA... Register December 7, 2009 that establishes Class E airspace at Fort A.P. Hill, VA. DATES: Effective Date..., Eastern Service Center, Federal Aviation Administration, P.O. Box 20636, Atlanta, Georgia 30320;...

  1. NACS: non-overlapping AP's caching scheme to reduce handoff in 802.11 wireless LAN

    CERN Document Server

    Tariq, Usman; Hong, Man-Pyo

    2011-01-01

    With the escalation of the IEEE 802.11 based wireless networks, voice over IP and analogous applications are also used over wireless networks. Recently, the wireless LAN systems are spaciously deployed for public Internet services. In public wireless LAN systems, reliable user authentication and mobility support are indispensable issues. When a mobile device budges out the range of one access point (AP) and endeavor to connect to new AP, it performs handoff. Contemporarily, PNC and SNC were proposed to propagate the MN context to the entire neighboring AP's on the wireless network with the help of neighbor graph. In this paper, we proposed a non-overlapping AP's caching scheme (NACS), which propagates the mobile node context to those AP's which do not overlap with the current AP. To capture the topology of non-overlapping AP's in the wireless network, non-overlapping graph (NOG) is generated at each AP. Simulation results shows that NACS reduces the signaling cost of propagating the MN context to the neighbor...

  2. Biological preconcentrator

    Science.gov (United States)

    Manginell, Ronald P.; Bunker, Bruce C.; Huber, Dale L.

    2008-09-09

    A biological preconcentrator comprises a stimulus-responsive active film on a stimulus-producing microfabricated platform. The active film can comprise a thermally switchable polymer film that can be used to selectively absorb and desorb proteins from a protein mixture. The biological microfabricated platform can comprise a thin membrane suspended on a substrate with an integral resistive heater and/or thermoelectric cooler for thermal switching of the active polymer film disposed on the membrane. The active polymer film can comprise hydrogel-like polymers, such as poly(ethylene oxide) or poly(n-isopropylacrylamide), that are tethered to the membrane. The biological preconcentrator can be fabricated with semiconductor materials and technologies.

  3. Biological Oceanography

    Science.gov (United States)

    Abbott, M. R.

    1984-01-01

    Within the framework of global biogeochemical cycles and ocean productivity, there are two areas that will be of particular interest to biological oceanography in the 1990s. The first is the mapping in space time of the biomass and productivity of phytoplankton in the world ocean. The second area is the coupling of biological and physical processes as it affects the distribution and growth rate of phytoplankton biomass. Certainly other areas will be of interest to biological oceanographers, but these two areas are amenable to observations from satellites. Temporal and spatial variability is a regular feature of marine ecosystems. The temporal and spatial variability of phytoplankton biomass and productivity which is ubiquitous at all time and space scales in the ocean must be characterized. Remote sensing from satellites addresses these problems with global observations of mesocale (2 to 20 days, 10 to 200 km) features over a long period of time.

  4. The Westinghouse AP1000 plant design: a generation III+ reactor with unique proven passive safety technology

    Energy Technology Data Exchange (ETDEWEB)

    Demetri, K. J.; Leipner, C. I.; Marshall, M. L., E-mail: demetrkj@westinghouse.com [Westinghouse Electric Company, 1000 Westinghouse Drive, Cranberry Township, PA 16066 (United States)

    2015-09-15

    The AP1000 plant is an 1100-M We pressurized water reactor with passive safety features and extensive plant simplifications and standardization that simplify construction, operation, maintenance, safety, and cost. The AP1000 plant is based on proven pressurized water reactor (PWR) technology, with an emphasis on safety features that rely solely on natural forces. These passive safety features are combined with simple, active, defense-in-depth systems used during normal plant operations which also provide the first level of defense against more probable events. This paper focuses on specific safety and licensing topics: the AP1000 plant robustness to be prepared for extreme events that may lead to catastrophic loss of infrastructure, such as the Fukushima Dai-ichi event, and the AP1000 plant compliance with the safety objectives for new plants. The first deployment of the AP1000 plant formally began in July 2007 when Westinghouse Electric Company and its consortium partner, the Shaw Group, signed contracts for four AP1000 units on coastal sites of Sanmen and Haiyang, China. Both sites have the planned ability to accommodate at least six AP1000 units; construction is largely concurrent for all four units. Additionally, the United States (U.S.) Nuclear Regulatory Commission (NRC) issued combined licenses (COLs) to allow Southern Nuclear Operating Company (SNC) and South Carolina Electric and Gas Company (SCE and G) to construct and operate AP1000 plants. Within this paper, the various factors that contribute to an unparalleled level of design, construction, delivery, and licensing certainty for any new AP1000 plant projects are described. These include: 1) How the AP1000 plant design development and reviews undertaken in the United States, China and Europe increase licensing certainty. 2) How the AP1000 passive plant robustness against extreme events that result in large loss of infrastructure further contributes to the licensing certainty in a post

  5. New Protein Vector ApE1 for Targeted Delivery of Anticancer Drugs

    Directory of Open Access Journals (Sweden)

    N. V. Pozdniakova

    2012-01-01

    Full Text Available A new chimeric gene ApE1 encoding the receptor-binding domain of the human alpha-fetoprotein fused to a sequence of 22 glutamic acid residues was constructed. A new bacterial producer strain E. coli SHExT7 ApE1 was selected for ApE1 production in a soluble state. A simplified method was developed to purify ApE1 from bacterial biomass. It was shown that the new vector protein selectively interacts with AFP receptors on the tumor cell surface and can be efficiently accumulated in tumor cells. In addition, ApE1 was shown to be stable in storage and during its chemical modification. An increased number of carboxyl groups in the molecule allows the production of cytotoxic compound conjugates with higher drug-loading capacity and enhanced tumor targeting potential.

  6. Glossary for the implementation of Health in All Policies (HiAP).

    Science.gov (United States)

    Freiler, Alix; Muntaner, Carles; Shankardass, Ketan; Mah, Catherine L; Molnar, Agnes; Renahy, Emilie; O'Campo, Patricia

    2013-12-01

    Health in All Policies (HiAP) is becoming increasingly popular as a governmental strategy to improve population health by coordinating action across health and non-health sectors. A variety of intersectoral initiatives may be used in HiAP that frame health determinants as the bridge between policies and health outcomes. The purpose of this glossary is to present concepts and terms useful in understanding the implementation of HiAP as a cross-sectoral policy. The concepts presented here were applied and elaborated over the course of case studies of HiAP in multiple jurisdictions, which used key informant interviews and the systematic review of literature to study the implementation of specific HiAP initiatives.

  7. Seed-specific aspartic proteinase FeAP12 from buckwheat (Fagopyrum esculentum Moench

    Directory of Open Access Journals (Sweden)

    Timotijević Gordana S.

    2010-01-01

    Full Text Available Aspartic proteinase gene (FeAP12 has been isolated from the cDNA library of developing buckwheat seeds. Analysis of its deduced amino acid sequence showed that it resembled the structure and shared high homology with typical plant aspartic proteinases (AP characterized by the presence of a plant-specific insert (PSI, unique among APs. It was shown that FeAP12 mRNA was not present in the leaves, roots, steam and flowers, but was seed-specifically expressed. Moreover, the highest levels of FeAP12 expression were observed in the early stages of seed development, therefore suggesting its potential role in nucellar degradation.

  8. Foreword: 18th Aps-Sccm and 24th Airapt

    Science.gov (United States)

    Collins, Gilbert; Moore, David S.; Yoo, Choong-Shik

    2014-05-01

    This second joint conference between the APS Topical Group on Shock Compression of Condensed Matter and the International Association for the Advancement of High Pressure Science and Technology (AIRAPT) demonstrates that static and dynamic compression of condensed matter continues to be a vibrant field of science and engineering. It is also by its nature an interdisciplinary field, incorporating chemistry, materials science, solid mechanics, plasma physics, and condensed matter physics, and utilizes theoretical, computational, and experimental tools. Recent years have brought about many advances in loading platforms, diagnostics, and computations that are leading to the emergence of many new avenues of research. These advances are also breathing new life into traditional topics such as equations of state, phase transformations, and chemistry at extreme conditions. The plenary lectures by Gennady Kanel, Karl Syassen, David Ceperley, Jon Eggert, Duck Young Kim, and Richard Kraus spanned the disciplines of static and dynamic high pressure physics and illustrated the breadth of the field. They also showed that interesting and important problems remain for researchers of the future to solve. The main guiding principal in the organization of this conference was to intertwine static and dynamical experimental alongside computational and theoretical studies of similar materials. To achieve this goal, we arranged the conference to include static, dynamic, and computational components in the same sessions, quite often taking presenters out of their comfort zone. The three special sessions on Deep Carbon Budget (organized by Giulia Galli and Rus Hemley), High Energy Density Materials (organized by Raymond Jeanloz and Jon Eggert), and Dynamic Response of Materials (organized by Yogendra Gupta and John Sarrao) furthered this guiding principal. We also endeavored to represent the breadth of static and dynamic high pressure science and technology, notably beyond that done at

  9. AP-2α Inhibits c-MYC Induced Oxidative Stress and Apoptosis in HaCaT Human Keratinocytes

    Directory of Open Access Journals (Sweden)

    Lei Yu

    2009-01-01

    AP-2 may have a direct effect on the c-myc gene. Chromatin immunoprecipitation assays demonstrated that AP-2 proteins bound to a cluster of AP-2 binding sites located within a 2 kb upstream regulatory region of c-myc These results suggest that the negative regulation of AP-2 on c-MYC activity was achieved through binding of AP-2 protein to the c-myc gene. The effects of AP-2 on c-MYC induced ROS accumulation and apoptosis in epidermal keratinocytes are likely to play an important role in cell growth, differentiation and carcinogenesis of the skin.

  10. Hidrocefalia após meningite - relato de caso

    Directory of Open Access Journals (Sweden)

    Tássia Sabbadin Mancilha

    2015-10-01

    Full Text Available A malformação do sistema nervoso central é um dos fatores de risco para meningite no período neonatal, a qual se caracteriza pela ocorrência de um processo infeccioso nas meninges, ocorrendo entre o nascimento e o 28º dia de vida. As complicações mais frequentemente associadas á meningite neonatal são a ventriculite, síndrome convulsiva, síndrome da secreção impropria do hormônio antidiurético, hidrocefalia, abcesso cerebral, hemorragia intracraniana, leucomalácia e coleção subdural. O objetivo do presente relato é descrever um caso de um paciente portador de uma má formação do sistema nervoso central, que apresentou aos 28 dias de vida quadro de meningite bacteriana que evoluiu para uma hidrocefalia. Após abordagem cirúrgica para colocação de DVE, seguida de complicações e troca de DVP, paciente apresenta suspeita de hipertensão intracraniana, quadro de crises convulsivas e atraso no desenvolvimento neuropsicomotor. A meningite no período neonatal é considerada uma patologia grave, cuja mortalidade varia de 15 a 50%. Sua incidência varia de 0,3 a 1 caso por 1000 nascidos vivos. Dentre os sobreviventes cerca de 50 a 75% evoluem com deficiência auditiva, deficiência visual, atraso no desenvolvimento neuropsicomotor, retardo mental, convulsões e hidrocefalia. Desta forma fica evidente, devido ao diagnóstico clinico ser difícil, a necessidade de manejo e tratamento precoce a fim de minimizar o prognóstico e as dificuldades que poderá apresentar o paciente no futuro.

  11. Renal effects of atriopeptin (AP) II in conscious SHR

    Energy Technology Data Exchange (ETDEWEB)

    Smits, J.F.; Debets, J.M.; Struyker-Boudier, H.A.; Daemen, M.J.

    1986-03-05

    Diuresis and natriuresis following APs has been suggested to depend upon increased renal blood flow (RBF). In a previous study, they found that APII infusions in conscious SHR decrease RBF. In this study, they report effects of APII on glomerular filtration rate (GFR) and renal excretory function in conscious SHR. Male SHR were equipped with arterial and venous catheters. Furthermore, a special catheter was implanted into the bladder to allow continuous urine sampling from conscious, freely moving rats. Animals were allowed at least 4 days to recover. In one group of SHR, APII (N=7) or saline (N=7) was infused to quantitate excretion of water, Na/sup +/, and K/sup +/. Two other groups of SHR (N=8 each) were infused with /sup 51/Cr-EDTA and /sup 125/I-PAH. GFR and renal plasma flow (ERPF) were calculated from urine and a mid-time plasma sample during APII or saline infusion. Following changes in infusion rates, urine from the first 5 min was discarded, after which a 10-min collection was made. APII (0.5-4 ..mu..g/kg/min) increased water, Na/sup +/ and K/sup +/ output maximally during 2 ..mu..g/kg/min. ERPF decreased from 10.7 +/- 1.1 ml/min to 7.9 +/- 0.5 ml/min. GFR did not change significantly. Filtration fraction increased from 24 +/- 1% to 33 +/- 2%. Fractional water excretion increased from 0.8 +/- 0.2% to 2.5 +/- 0.7%. Saline infusions did not affect renal function. The results indicate that in conscious SHR, APII causes diuresis and natriuresis which does not depend upon increased GFR, but, rather, upon a primary tubular effect.

  12. Ripped from the Headlines: Using Current Events and Deliberative Democracy to Improve Student Performance in and Perceptions of Nonmajors Biology Courses

    Directory of Open Access Journals (Sweden)

    Heather Nicole Tinsley

    2016-12-01

    Full Text Available Despite the importance of scientific literacy, many foundational science courses are plagued by low student engagement and performance. In an attempt to improve student outcomes, an introductory biology course for nonscience majors was redesigned to present the course content within the framework of current events and deliberative democratic exercises. During each instructional unit of the redesigned course, students were presented with a highly publicized policy question rooted in biological principles and currently facing lawmakers. Working in diverse groups, students sought out the information that was needed to reach an educated, rationalized decision. This approach models civic engagement and demonstrates the real-life importance of science to nonscience majors. The outcomes from two semesters in which the redesign were taught were compared with sections of the course taught using traditional pedagogies. When compared with other versions of the same course, presenting the course content within a deliberative democratic framework proved to be superior for increasing students’ knowledge gains and improving students’ perceptions of biology and its relevance to their everyday lives. These findings establish deliberative democracy as an effective pedagogical strategy for nonmajors biology.

  13. (Biological dosimetry)

    Energy Technology Data Exchange (ETDEWEB)

    Preston, R.J.

    1990-12-17

    The traveler attended the 1st International Conference on Biological Dosimetry in Madrid, Spain. This conference was organized to provide information to a general audience of biologists, physicists, radiotherapists, industrial hygiene personnel and individuals from related fields on the current ability of cytogenetic analysis to provide estimates of radiation dose in cases of occupational or environmental exposure. There is a growing interest in Spain in biological dosimetry because of the increased use of radiation sources for medical and occupational uses, and with this the anticipated and actual increase in numbers of overexposure. The traveler delivered the introductory lecture on Biological Dosimetry: Mechanistic Concepts'' that was intended to provide a framework by which the more applied lectures could be interpreted in a mechanistic way. A second component of the trip was to provide advice with regard to several recent cases of overexposure that had been or were being assessed by the Radiopathology and Radiotherapy Department of the Hospital General Gregorio Maranon'' in Madrid. The traveler had provided information on several of these, and had analyzed cells from some exposed or purportedly exposed individuals. The members of the biological dosimetry group were referred to individuals at REACTS at Oak Ridge Associated Universities for advice on follow-up treatment.

  14. Scaffolded biology.

    Science.gov (United States)

    Minelli, Alessandro

    2016-09-01

    Descriptions and interpretations of the natural world are dominated by dichotomies such as organism vs. environment, nature vs. nurture, genetic vs. epigenetic, but in the last couple of decades strong dissatisfaction with those partitions has been repeatedly voiced and a number of alternative perspectives have been suggested, from perspectives such as Dawkins' extended phenotype, Turner's extended organism, Oyama's Developmental Systems Theory and Odling-Smee's niche construction theory. Last in time is the description of biological phenomena in terms of hybrids between an organism (scaffolded system) and a living or non-living scaffold, forming unit systems to study processes such as reproduction and development. As scaffold, eventually, we can define any resource used by the biological system, especially in development and reproduction, without incorporating it as happens in the case of resources fueling metabolism. Addressing biological systems as functionally scaffolded systems may help pointing to functional relationships that can impart temporal marking to the developmental process and thus explain its irreversibility; revisiting the boundary between development and metabolism and also regeneration phenomena, by suggesting a conceptual framework within which to investigate phenomena of regular hypermorphic regeneration such as characteristic of deer antlers; fixing a periodization of development in terms of the times at which a scaffolding relationship begins or is terminated; and promoting plant galls to legitimate study objects of developmental biology.

  15. Biology Notes.

    Science.gov (United States)

    School Science Review, 1984

    1984-01-01

    Presents information on the teaching of nutrition (including new information relating to many current O-level syllabi) and part 16 of a reading list for A- and S-level biology. Also includes a note on using earthworms as a source of material for teaching meiosis. (JN)

  16. Biology Notes.

    Science.gov (United States)

    School Science Review, 1981

    1981-01-01

    Outlines a variety of laboratory procedures, techniques, and materials including construction of a survey frame for field biology, a simple tidal system, isolation and applications of plant protoplasts, tropisms, teaching lung structure, and a key to statistical methods for biologists. (DS)

  17. Marine Biology

    Science.gov (United States)

    Dewees, Christopher M.; Hooper, Jon K.

    1976-01-01

    A variety of informational material for a course in marine biology or oceanology at the secondary level is presented. Among the topics discussed are: food webs and pyramids, planktonic blooms, marine life, plankton nets, food chains, phytoplankton, zooplankton, larval plankton and filter feeders. (BT)

  18. Science Café Course: An Innovative Means of Improving Communication Skills of Undergraduate Biology Majors

    Directory of Open Access Journals (Sweden)

    Anna Goldina

    2013-12-01

    Full Text Available To help bridge the increasing gap between scientists and the public, we developed an innovative two-semester course, called Science Café. In this course undergraduate biology majors learn to develop communication skills to be better able to explain science concepts and current developments in science to non-scientists. Students develop and host outreach events on various topics relevant to the community, thereby increasing interactions between budding scientists and the public. Such a Science Cafe course emphasizes development of science communication skills early, at the undergraduate level and empowers students to use their science knowledge in every day interactions with the public to increase science literacy, get involved in the local community and engage the public in a dialogue on various pressing science issues. We believe that undergraduate science majors can be great ambassadors for science and are often overlooked since many aspire to go on to medical/veterinary/pharmacy schools. However, science communication skills are especially important for these types of students because when they become healthcare professionals, they will interact with the public as part of their everyday jobs and can thus be great representatives for the field.

  19. Science café course: an innovative means of improving communication skills of undergraduate biology majors.

    Science.gov (United States)

    Goldina, Anna; Weeks, Ophelia I

    2014-05-01

    To help bridge the increasing gap between scientists and the public, we developed an innovative two-semester course called Science Café. In this course, undergraduate biology majors learn to develop communication skills to be better able to explain science concepts and current developments in science to non-scientists. Students develop and host outreach events on various topics relevant to the community, thereby increasing interactions between budding scientists and the public. Such a Science Café course emphasizes development of science communication skills early, at the undergraduate level, and empowers students to use their science knowledge in everyday interactions with the public to increase science literacy, get involved in the local community and engage the public in a dialogue on various pressing science issues. We believe that undergraduate science majors can be great ambassadors for science and are often overlooked since many aspire to go on to medical/veterinary/pharmacy schools. However, science communication skills are especially important for these types of students because when they become healthcare professionals, they will interact with the public as part of their everyday jobs and can thus be great representatives for the field.

  20. Knockdown of CDK2AP1 in primary human fibroblasts induces p53 dependent senescence.

    Directory of Open Access Journals (Sweden)

    Khaled N Alsayegh

    Full Text Available Cyclin Dependent Kinase-2 Associated Protein-1 (CDK2AP1 is known to be a tumor suppressor that plays a role in cell cycle regulation by sequestering monomeric CDK2, and targeting it for proteolysis. A reduction of CDK2AP1 expression is considered to be a negative prognostic indicator in patients with oral squamous cell carcinoma and also associated with increased invasion in human gastric cancer tissue. CDK2AP1 overexpression was shown to inhibit growth, reduce invasion and increase apoptosis in prostate cancer cell lines. In this study, we investigated the effect of CDK2AP1 downregulation in primary human dermal fibroblasts. Using a short-hairpin RNA to reduce its expression, we found that knockdown of CDK2AP1 in primary human fibroblasts resulted in reduced proliferation and in the induction of senescence associated beta-galactosidase activity. CDK2AP1 knockdown also resulted in a significant reduction in the percentage of cells in the S phase and an accumulation of cells in the G1 phase of the cell cycle. Immunocytochemical analysis also revealed that the CDK2AP1 knockdown significantly increased the percentage of cells that exhibited γ-H2AX foci, which could indicate presence of DNA damage. CDK2AP1 knockdown also resulted in increased mRNA levels of p53, p21, BAX and PUMA and p53 protein levels. In primary human fibroblasts in which p53 and CDK2AP1 were simultaneously downregulated, there was: (a no increase in senescence associated beta-galactosidase activity, (b decrease in the number of cells in the G1-phase and increase in number of cells in the S-phase of the cell cycle, and (c decrease in the mRNA levels of p21, BAX and PUMA when compared with CDK2AP1 knockdown only fibroblasts. Taken together, this suggests that the observed phenotype is p53 dependent. We also observed a prominent increase in the levels of ARF protein in the CDK2AP1 knockdown cells, which suggests a possible role of ARF in p53 stabilization following CDK2AP1

  1. In situ gel-forming AP-57 peptide delivery system for cutaneous wound healing.

    Science.gov (United States)

    Li, Xiaoling; Fan, Rangrang; Tong, Aiping; Yang, Meijia; Deng, Jiaojiao; Zhou, Liangxue; Zhang, Xiaoning; Guo, Gang

    2015-11-10

    In situ gel-forming system as local drug delivery system in dermal traumas has generated a great interest. Accumulating evidence shows that antimicrobial peptides play pivotal roles in the process of wound healing. Here in this study, to explore the potential application of antimicrobial peptide in wound healing, biodegradable poly(L-lactic acid)-Pluronic L35-poly(L-lactic acid) (PLLA-L35-PLLA) was developed at first. Then based on this polymer, an injectable in situ gel-forming system composed of human antimicrobial peptides 57 (AP-57) loaded nanoparticles and thermosensitive hydrogel was prepared and applied for cutaneous wound healing. AP-57 peptides were enclosed with biocompatible nanoparticles (AP-57-NPs) with high drug loading and encapsulation efficiency. AP-57-NPs were further encapsulated in a thermosensitive hydrogel (AP-57-NPs-H) to facilitate its application in cutaneous wound repair. As a result, AP-57-NPs-H released AP-57 in an extended period and exhibited quite low cytotoxicity and high anti-oxidant activity in vitro. Moreover, AP-57-NPs-H was free-flowing liquid at room temperature, and can form non-flowing gel without any crosslink agent upon applied on the wounds. In vivo wound healing assay using full-thickness dermal defect model of SD rats indicated that AP-57-NPs-H could significantly promote wound healing. At day 14 after operation, AP-57-NPs-H treated group showed nearly complete wound closure of 96.78 ± 3.12%, whereas NS, NPs-H and AP-57-NPs group recovered by about 68.78 ± 4.93%, 81.96 ± 3.26% and 87.80 ± 4.62%, respectively. Histopathological examination suggested that AP-57-NPs-H could promote cutaneous wound healing through enhancing granulation tissue formation, increasing collagen deposition and promoting angiogenesis in the wound tissue. Therefore, AP-57-NPs-H might have potential application in wound healing.

  2. Analysis of AP 1000 ReactorPower Control System%AP1000反应堆控制系统特点分析

    Institute of Scientific and Technical Information of China (English)

    张小冬; 刘琳

    2011-01-01

    通过对核电机组常见的控制模式以及AP1000采用的控制模式的介绍,总结出各种模式的优缺点,并分析AP1000所采取的控制模式的先进性,对三门核电厂首台机组及后续机组的运行控制模式提出建议;还结合AP1000反应堆功率控制系统的特点,对在正常运行期间可能遇到的问题加以分析,并提出相应的对策.%The reactor power control modes applied by common nuclear power plants and AP1000 is introduced briefly. The advantages and shortcomings are compared and summarized. Advice is also made to the control strategy of the first and continued SMNPC plants. Additionally,the characteristics of the AP1000 reactor control system are summarized and problems which the operators will probably encounter are also analyzed.

  3. PfAP2Tel, harbouring a non-canonical DNA-binding AP2 domain, binds to Plasmodium falciparum telomeres.

    Science.gov (United States)

    Sierra-Miranda, Miguel; Vembar, Shruthi S; Delgadillo, Dulce María; Ávila-López, P A; Vargas, Miguel; Hernandez-Rivas, Rosaura

    2017-04-04

    The telomeres of the malaria parasite Plasmodium falciparum are essential not only for chromosome end maintenance during blood stage development in humans but also to generate genetic diversity by facilitating homologous recombination of subtelomeric, multigene virulence families such as var and rifin. However, other than the telomerase PfTERT, proteins that act at P. falciparum telomeres are poorly characterized. To isolate components that bind to telomeres, we performed oligonucleotide pulldowns and electromobility shift assays with a telomeric DNA probe and identified a non-canonical member of the ApiAP2 family of transcription factors, PfAP2Tel (encoded by PF3D7_0622900), as a component of the P. falciparum telomere-binding protein complex. PfAP2Tel is expressed throughout the intra-erythrocytic life cycle and localizes to the nuclear periphery, co-localizing with telomeric clusters. Furthermore, EMSAs using the recombinant protein demonstrated direct binding of PfAP2Tel to telomeric repeats in vitro, while genome-wide chromatin immunoprecipitation followed by next generation sequencing (ChIP-seq) corroborated the high specificity of this protein to telomeric ends of all 14 chromosomes in vivo. Taken together, our data describe a novel function for ApiAP2 proteins at chromosome ends and open new avenues to study the molecular machinery that regulates telomere function in P. falciparum.

  4. Ectopic expression of Jatropha curcas APETALA1 (JcAP1 caused early flowering in Arabidopsis, but not in Jatropha

    Directory of Open Access Journals (Sweden)

    Mingyong Tang

    2016-04-01

    Full Text Available Jatropha curcas is a promising feedstock for biofuel production because Jatropha oil is highly suitable for the production of biodiesel and bio-jet fuels. However, Jatropha exhibits a low seed yield as a result of unreliable and poor flowering. APETALA1 (AP1 is a floral meristem and organ identity gene in higher plants. The flower meristem identity genes of Jatropha have not yet been identified or characterized. To better understand the genetic control of flowering in Jatropha, an AP1 homolog (JcAP1 was isolated from Jatropha. An amino acid sequence analysis of JcAP1 revealed a high similarity to the AP1 proteins of other perennial plants. JcAP1 was expressed in inflorescence buds, flower buds, sepals and petals. The highest expression level was observed during the early developmental stage of the flower buds. The overexpression of JcAP1 using the cauliflower mosaic virus (CaMV 35S promoter resulted in extremely early flowering and abnormal flowers in transgenic Arabidopsis plants. Several flowering genes downstream of AP1 were up-regulated in the JcAP1-overexpressing transgenic plant lines. Furthermore, JcAP1 overexpression rescued the phenotype caused by the Arabidopsis AP1 loss-of-function mutant ap1-11. Therefore, JcAP1 is an ortholog of AtAP1, which plays a similar role in the regulation of flowering in Arabidopsis. However, the overexpression of JcAP1 in Jatropha using the same promoter resulted in little variation in the flowering time and floral organs, indicating that JcAP1 may be insufficient to regulate flowering by itself in Jatropha. This study helps to elucidate the function of JcAP1 and contributes to the understanding of the molecular mechanisms of flower development in Jatropha.

  5. [An investigation of fast thermolysis of GAP/AP system by FTIR spectroscopy].

    Science.gov (United States)

    Zhang, La-Ying; Liu, Zi-Ru; Wang, Xiao-Hong; Heng, Shu-Yun; Shao, Ying-Hui; Ding, Li

    2013-01-01

    The rapid pyrolysis of GAP/AP system under simulated combustion conditions was investigated by an on-line analysis, i. e. so called T-Jump/FTIR. The results show that the compositions of the main gaseous products for pyrolysis are changed, in comparison with the pyrolyses of single, which indicates that the interactions occur between the components of GAP/AP system. From an obvious effect of pressure on the main gaseous products for GAP/AP pyrolysis it is shown that the interactions between GAP and AP components arise from the gaseous products of AP and both the reactions in gas phase and in gas/condensed phase occur in the GAP/AP mixed system. The interactions between GAP and AP are not affected by test temperature. It is considered that the real time analysis of gaseous products of energetic material pyrolysis under simulated combustion conditions would be carried out by T-Jump/FTIR on-line analysis technique and from microcosmic reaction a technical approach used to explore the rapid pyrolysis of energetic materials and interactions between their components at high temperature and pressure would be developed by the on-line analysis technique from microcosmic reaction.

  6. The Role of the Clathrin Adaptor AP-1: Polarized Sorting and Beyond

    Directory of Open Access Journals (Sweden)

    Fubito Nakatsu

    2014-11-01

    Full Text Available The selective transport of proteins or lipids by vesicular transport is a fundamental process supporting cellular physiology. The budding process involves cargo sorting and vesicle formation at the donor membrane and constitutes an important process in vesicular transport. This process is particularly important for the polarized sorting in epithelial cells, in which the cargo molecules need to be selectively sorted and transported to two distinct destinations, the apical or basolateral plasma membrane. Adaptor protein (AP-1, a member of the AP complex family, which includes the ubiquitously expressed AP-1A and the epithelium-specific AP-1B, regulates polarized sorting at the trans-Golgi network and/or at the recycling endosomes. A growing body of evidence, especially from studies using model organisms and animals, demonstrates that the AP-1-mediated polarized sorting supports the development and physiology of multi-cellular units as functional organs and tissues (e.g., cell fate determination, inflammation and gut immune homeostasis. Furthermore, a possible involvement of AP-1B in the pathogenesis of human diseases, such as Crohn’s disease and cancer, is now becoming evident. These data highlight the significant contribution of AP-1 complexes to the physiology of multicellular organisms, as master regulators of polarized sorting in epithelial cells.

  7. Postural tachycardia syndrome (POTS) and other autonomic disorders in antiphospholipid (Hughes) syndrome (APS).

    Science.gov (United States)

    Schofield, J R; Blitshteyn, S; Shoenfeld, Y; Hughes, G R V

    2014-06-01

    Antiphospholipid syndrome (APS) is an autoimmune hypercoagulable disorder that has been shown to cause a large number of cardiac and neurological manifestations. Two recent studies have demonstrated abnormalities in cardiovascular autonomic function testing in APS patients without other cardiovascular or autoimmune disease. However, an association between autonomic disorders such as postural tachycardia syndrome and APS has not previously been described. Data were obtained by retrospective chart review. We identified 15 patients who have been diagnosed with APS and an autonomic disorder. The median age of the patients at the time of data analysis was 39 years. The autonomic disorders seen in these patients included postural tachycardia syndrome, neurocardiogenic syncope and orthostatic hypotension. The majority of patients (14/15) were female and the majority (14/15) had non-thrombotic neurological manifestations of APS, most commonly migraine, memory loss and balance disorder. Many also had livedo reticularis (11/15) and Raynaud's phenomenon (nine of 15). In some patients, the autonomic manifestations improved with anticoagulation and/or anti-platelet therapy; in others they did not. Two patients with postural tachycardia syndrome who failed to improve with the usual treatment of APS have been treated with intravenous immunoglobulin with significant improvement in their autonomic symptoms. We believe that autonomic disorders in APS may represent an important clinical association with significant implications for treatment. © The Author(s) 2014 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav.

  8. Culturally relevant inquiry-based laboratory module implementations in upper-division genetics and cell biology teaching laboratories.

    Science.gov (United States)

    Siritunga, Dimuth; Montero-Rojas, María; Carrero, Katherine; Toro, Gladys; Vélez, Ana; Carrero-Martínez, Franklin A

    2011-01-01

    Today, more minority students are entering undergraduate programs than ever before, but they earn only 6% of all science or engineering PhDs awarded in the United States. Many studies suggest that hands-on research activities enhance students' interest in pursuing a research career. In this paper, we present a model for the implementation of laboratory research in the undergraduate teaching laboratory using a culturally relevant approach to engage students. Laboratory modules were implemented in upper-division genetics and cell biology courses using cassava as the central theme. Students were asked to bring cassava samples from their respective towns, which allowed them to compare their field-collected samples against known lineages from agricultural stations at the end of the implementation. Assessment of content and learning perceptions revealed that our novel approach allowed students to learn while engaged in characterizing Puerto Rican cassava. In two semesters, based on the percentage of students who answered correctly in the premodule assessment for content knowledge, there was an overall improvement of 66% and 55% at the end in the genetics course and 24% and 15% in the cell biology course. Our proposed pedagogical model enhances students' professional competitiveness by providing students with valuable research skills as they work on a problem to which they can relate.

  9. The AP-3 adaptor complex is required for vacuolar function in Arabidopsis

    Institute of Scientific and Technical Information of China (English)

    Maria Zwiewka; Elena Feraru; Barbara M(o)ller; Inhwan Hwang; Mugurel I Feraru; Jürgen Kleine-Vehn; Dolf Weijers; Ji(n) Friml

    2011-01-01

    Subcellular trafficking is required for a multitude of functions in eukaryotic cells.It involves regulation of cargo sorting,vesicle formation,trafficking and fusion processes at multiple levels.Adaptor protein (AP) complexes are key regulators of cargo sorting into vesicles in yeast and mammals but their existence and function in plants have not been demonstrated.Here we report the identification of the protein-affected trafficking 4 (pat4) mutant defective in the putative δ subunit of the AP-3 complex.pat4 and pat2,a mutant isolated from the same GFP imaging-based forward genetic screen that lacks a functional putative AP-3 β,as well as dominant negative AP-3 μ transgenic lines display undistinguishable phenotypes characterized by largely normal morphology and development,but strong intracellular accumulation of membrane proteins in aberrant vacuolar structures.All mutants are defective in morphology and function of lytic and protein storage vacuoles (PSVs) but show normal sorting of reserve proteins to PSVs.Immunoprecipitation experiments and genetic studies revealed tight functional and physical associations of putative AP-3 β and AP-3 δ subunits.Furthermore,both proteins are closely linked with putative AP-3 μ and σ subunits and several components of the clathrin and dynamin machineries.Taken together,these results demonstrate that AP complexes,similar to those in other eukaryotes,exist in plants,and that AP-3 plays a specific role in the regulation of biogenesis and function of vacuoles in plant cells.

  10. Mesoscopic biology

    Indian Academy of Sciences (India)

    G V Shivashankar

    2002-02-01

    In this paper we present a qualitative outlook of mesoscopic biology where the typical length scale is of the order of nanometers and the energy scales comparable to thermal energy. Novel biomolecular machines, governed by coded information at the level of DNA and proteins, operate at these length scales in biological systems. In recent years advances in technology have led to the study of some of the design principles of these machines; in particular at the level of an individual molecule. For example, the forces that operate in molecular interactions, the stochasticity involved in these interactions and their spatio-temporal dynamics are beginning to be explored. Understanding such design principles is opening new possibilities in mesoscopic physics with potential applications.

  11. Adenocarcinoma primario del apéndice cecal

    Directory of Open Access Journals (Sweden)

    Rolando Galano Urgellés

    1998-08-01

    Full Text Available Se realizó un estudio longitudinal y prospectivo de 5 pacientes operados de cáncer del apéndice cecal, atendidos en una consulta de seguimiento habilitada para pacientes intervenidos de cáncer colorrectal. El análisis destacó que la edad más frecuente se halló por encima de la 5ta. década de la vida, y que hubo predominio del sexo masculino. Se practicó una reintervención durante el seguimiento; como terapéutica se utilizó el 5-fluoracilo; hubo un predominio absoluto del adenocarcinoma como variedad histológica; el diagnóstico de la entidad siempre se confirmó por Anatomía Patológica. La muestra representa el 1,2 % del total de casos de nuestra consulta y el 0,4 % de los pacientes apendicectomizados en nuestro Centro durante el período de estudio. Se recomienda insistir en el pesquisaje masivo de aquellos grupos de riesgo por parte del nivel primario de atención, para detectar precozmente la entidad y, por ende, mejorar los resultados quirúrgicos y de seguimiento posoperatorio, así como aplicar terapéutica de Sieracki en esta entidadA lengthwise and prospective study of 5 patients operated from appendix ceci cancer, who were attended in the follow-up consultation room for patients operated from colo-rectal cancer was performed. The analysis underlined that the most frequent age was over 50 years-old and males were predominant. During the follow-up period, one of the patients was operated again and treated with 5-fluoroacyl; adenocarcinoma prevailed as hystological variant and its diagnosis was always confirmed by the Pathological Anatomy Department. The sample of 5 patients represented 1.2 % of the cases attending our consultation room and 0.4 % of appendectomy-operated patients in our hospital during the studied period. The massive continuos screening of risk groups at the primary care level is recommended so as to early detect adenocarcinoma and thus improve the surgical and posoperative follow-up results and apply the

  12. Medidas orofaciais em nipobrasileiros após crescimento puberal

    Directory of Open Access Journals (Sweden)

    Tassiana Barbeiro Fragoso de Sá

    2013-04-01

    Full Text Available OBJETIVO: descrever medidas orofaciais em nipobrasileiros após crescimento puberal e verificar diferenças entre medidas de mulheres negras, brancas e nipobrasileiras. MÉTODO: a casuística foi composta de 90 sujeitos japoneses ou descendentes de japoneses, sem histórico de miscigenação, ambos os sexos, entre 20 e 50 anos, sem queixas ou atendimentos fonoaudiológicos atuais ou prévios em motricidade orofacial, sem tratamento ortodôntico atual e sem alteração cognitiva. As medidas avaliadas foram: lábio superior, lábio inferior, filtro, terço superior da face, terço médio da face, terço inferior da face, lados da face, abertura máxima de boca e abertura máxima de boca com a língua na papila. O instrumento utilizado foi o paquímetro eletrônico digital da marca Jomarca. Os dados foram comparados com os achados na literatura referentes às mulheres negras e brancas. RESULTADOS: os dados coletados mostraram homogeneidade entre as medidas; os valores para todas as medidas foram maiores nos homens, com relevância estatística positiva. As diferenças mais significantes entre as negras, brancas e nipobrasileiras foram: terço superior e filtro das nipobrasileiras menor do que de negras e brancas. Para o terço médio não houve diferença estatística significante. O lábio superior das nipobrasileiras é maior do que das brancas e o lábio inferior das nipobrasileiras menor do que das negras. Lados da face sem diferenças estatísticas. CONCLUSÃO: foi possível verificar que as médias das medidas foram sempre maiores nos homens, que houve relações significantes entre algumas medidas orofaciais e que houve diferença estatisticamente significante entre as estruturas das brancas, negras e nipobrasileiras.

  13. Após a tragédia

    Directory of Open Access Journals (Sweden)

    Jean-Luc Nancy

    2014-09-01

    Full Text Available Aqui se discute a necessidade do abandono da Tragédia e a impossibilidade desse abandono, porque na tragédia temos a expressão do luto da história e, na verdade, do niilismo Ocidental. Pensar “após-tragédia” é, na verdade, pensar aporisticamente os impasses a que a modernidade chegou.Tradução: João Camillo Penna Abstract: The essay contemplates the need to abandon Tragedy and the simultaneous impossibility of this abandonment. In Tragedy there is the expression of History’s grief and, in truth, of Occident’s Nihilism. Thinking a “post-tragedy” actually means thinking aporistically the blockage points towards which modernity arrived.Keywords: Grief; Tragedy; Impossibility of Tragedy; Time and History; Nihilism. Jean-Luc Nancy é professor de filsofia na Universidade de Estrasburgo. Um dos grandes nomes da filosofia contemporânea, tem extensa obra, ressaltando-se os volumes que escreveu com Philippe Lacoue-Labarthe, como O absoluto literário, O mito nazi, A imitação dos modernos, entre vários outros. Individualmente, pode-se destacar Ego sum, 1979; La communauté désoeuvrée, 1983; L’oubli de la philosophie, 1986; Corpus, 1992; L’Intrus, 2000; Noli me tangere, 2003; L’Équivalence des catastrophes, 2012. João Camillo Penna é professor de literatura comparada e teoria literária na UFRJ. Autor de artigos na área de crítica da violência, de um livro de poesias, Parador (poesia, Móbile editorial, 2011, e de Escritos da sobrevivência (ensaios, 7Letras, 2013. Participa como tradutor e revisor do volume de ensaios de Jean-Luc Nancy Demanda. Literatura e filosofia. (EdUFSC, 2014, no prelo e-mail: jcamillopenna@gmail.com

  14. Marine biology

    Energy Technology Data Exchange (ETDEWEB)

    Thurman, H.V.; Webber, H.H.

    1984-01-01

    This book discusses both taxonomic and ecological topics on marine biology. Full coverage of marine organisms of all five kingdoms is provided, along with interesting and thorough discussion of all major marine habitats. Organization into six major parts allows flexibility. It also provides insight into important topics such as disposal of nuclear waste at sea, the idea that life began on the ocean floor, and how whales, krill, and people interact. A full-color photo chapter reviews questions, and exercises. The contents are: an overview marine biology: fundamental concepts/investigating life in the ocean; the physical ocean, the ocean floor, the nature of water, the nature and motion of ocean water; general ecology, conditions for life in the sea, biological productivity and energy transfer; marine organisms; monera, protista, mycota and metaphyta; the smaller marine animals, the large animals marine habitats, the intertidal zone/benthos of the continental shelf, the photic zone, the deep ocean, the ocean under stress, marine pollution, appendix a: the metric system and conversion factors/ appendix b: prefixes and suffixes/ appendix c: taxonomic classification of common marine organisms, and glossary, and index.

  15. 241-AP Tank Farm Construction Extent of Condition Review for Tank Integrity

    Energy Technology Data Exchange (ETDEWEB)

    Barnes, Travis J.; Gunter, Jason R.; Reeploeg, Gretchen E.

    2014-04-04

    This report provides the results of an extent of condition construction history review for the 241-AP tank farm. The construction history of the 241-AP tank farm has been reviewed to identify issues similar to those experienced during tank AY-102 construction. Those issues and others impacting integrity are discussed based on information found in available construction records, using tank AY-102 as the comparison benchmark. In the 241-AP tank farm, the sixth double-shell tank farm constructed, tank bottom flatness, refractory material quality, post-weld stress relieving, and primary tank bottom weld rejection were improved.

  16. A Weighted K-AP Query Method for RSSI based Indoor Positioning

    DEFF Research Database (Denmark)

    Huo, Huan; Liu, Xiufeng; Li, Jifeng

    2016-01-01

    . To eliminate the positioning error caused by instable RSSI value, WF-SKL can filter the noise AP via online AP selection, meanwhile it also reduces the computation load. WF-SKL utilizes LCS algorithm to find out the measurement between the nearest neighbors, and it proposes K-AP (P,Q) nearest neighbor queries...... between two sets based on Map-Reduce framework. The algorithm can find out K (P,Q) nearest positions and weighted them for re-positioning to accelerate the matching speed between online data and offline data, and also improve the efficiency of positioning. According to a large scale positioning...

  17. Panel Discussion : Report of the APS Ad-Hoc Committee on LGBT Issues

    Science.gov (United States)

    Atherton, Tim; Barthelemy, Ramon; Garmon, Savannah; Reeves, Kyle; APS Ad-Hoc Committee on LGBT Issues Team

    Following the presentation of the findings and recommendations of the APS Ad-Hoc Committee on LGBT Issues (C-LGBT) by Committee Chair Michael Falk, a panel discussion will be held featuring several members of the committee. The discussion will focus on how APS can best ensure the recommendations of the committee are carried out in a timely fashion and other ideas on future APS efforts toward LGBT inclusion in physics. Discussion topics will also include the research and other input that shaped the committee's findings and recommendations.

  18. On the structure of AP-4 responsive element in the LTR of Jembrana disease virus

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    Previous studies with deletion and sequence analysis of JDV LTR showed that there is a putative AP-4 responsive element in LTR. By antisense transient assay and gel shifting assay, we for the first time demonstrated that AP-4 modulated JDV gene expression by binding DNA directly to bovine cells. The results, derived from site-directed mutagenesis experiments, suggest that the six base pairs of AP-4 binding site (CAGCTG) have different effects on JDV gene expression. When the first two base pairs changed to GC, JDV gene expression is severely decreased.

  19. Technical Steps to Support Nuclear Arsenal Downsizing: A Report by the APS Panel on Public Affairs

    Science.gov (United States)

    2010-02-01

    Technical Steps to Support Nuclear Arsenal Downsizing A RepoRt by the ApS pAnel on public AffAiRS TM Report Documentation Page Form ApprovedOMB No...00-2010 to 00-00-2010 4. TITLE AND SUBTITLE Technical Steps to Support Nuclear Arsenal Downsizing : A Report by the APS Panel on Public Affairs 5a...Contents Executive Summary 2 Introduction 3 Verifying Downsizing and Dismantlement 5 Sustaining Capability and Expertise 15 Ensuring Peaceful Uses of

  20. Osiguravanje međuoperabilnosti za Internet stvari: iskustvo s testiranjem CoAP protokola

    OpenAIRE

    Chen, Nanxing; Viho, César; Baire, Anthony; Huang, Xiaohong; Zha, Jiexi

    2014-01-01

    Constrained Application Protocol (CoAP) je specijalizirani prijenosni protokol, dizajniran za realizaciju međuoperabilnosti uz ograničene mrežame i čvorove za primjene poput pametne energije, automatizacije u zgradarstvu itd. Kao važan i sveprisutan protokol za Internet stvari CoAP bi mogao biti implementiran kod velikog broja pametnih uređaja kako bi se ostvarile kooperativne usluge. Zbog toga je od velike važnosti postic´i visoku razinu međuoperabilnosti CoAP implementacija. U tom kontekstu...

  1. CoCoA+: an advanced congestion control mechanism for CoAP

    OpenAIRE

    Betzler, August; Gómez Montenegro, Carlos; Seyfettin Demirkol, Ilker; Paradells Aspas, Josep

    2015-01-01

    The Constrained Application Protocol (CoAP) has been designed by the Internet Engineering Task Force (IETF) for Internet of Things (IoT) devices. Due to the limited radio channel capacities and hardware resources of such devices, congestion can be a serious problem. CoAP addresses this important issue with a basic congestion control mechanism. CoCoA, an Internet-Draft proposal, introduced alternative congestion control mechanisms for CoAP. Yet, there has been limited evaluation of these conge...

  2. Glossary for the implementation of Health in All Policies (HiAP)

    OpenAIRE

    Freiler, Alix; Muntaner, Carles; Shankardass, Ketan; Mah, Catherine L.; Molnar, Agnes; Renahy, Emilie; O'Campo, Patricia

    2013-01-01

    Health in All Policies (HiAP) is becoming increasingly popular as a governmental strategy to improve population health by coordinating action across health and non-health sectors. A variety of intersectoral initiatives may be used in HiAP that frame health determinants as the bridge between policies and health outcomes. The purpose of this glossary is to present concepts and terms useful in understanding the implementation of HiAP as a cross-sectoral policy. The concepts presented here were a...

  3. Structural Biology Fact Sheet

    Science.gov (United States)

    ... Home > Science Education > Structural Biology Fact Sheet Structural Biology Fact Sheet Tagline (Optional) Middle/Main Content Area ​Other Fact Sheets What is structural biology? Structural biology is the study of how biological ...

  4. Ap( Bn,dV)上具有BT符号的Toeplitz算子%Toeplitz operators on Ap (Bn, dV) with symbol in BT

    Institute of Scientific and Technical Information of China (English)

    何莉; 曹广福

    2011-01-01

    研究了高维Bergman空间Ap(Bn,dV)(1 <p<∞)上具有BT符号的Toeplitz算子,利用Toeplitz算子的Berezin变换讨论了Toeplitz算子的有界性,得到了Ap(Bn,dV)上具有BT符号的Toeplitz算子的范数和本性范数的估计,推广了MIAO和ZHENG在Bergman空间LP(D,dA)上对具有BT符号的Toeplitz算子的范数和本性范数进行估计的结论.

  5. A CMOS vision chip for a contrast-enhanced image using a logarithmic APS and a switch-selective resistive network

    Science.gov (United States)

    Kong, Jae-Sung; Kim, Sang-Heon; Sung, Dong-Kyu; Seo, Sang-Ho; Shin, Jang-Kyoo

    2007-02-01

    In this paper, a vision chip for a contrast-enhanced image based on a structure of a biological retina is introduced. The key advantage of this structure is high speed of signal processing. In a conventional active pixel sensor (APS), the charge accumulation time limits its operation speed. In order to enhance the speed, a logarithmic APS was applied to the vision chip. By applying a MOS-type photodetector to the logarithmic APS, we could achieve sufficient output swing for the vision chip in natural illumination condition. In addition, a CMOS buffer circuit, a common drain amplifier, is commonly used for both raw and smoothed images by using additional switches. By using the switch-selective resistive network, the total number of MOSFETs for a unit pixel and the fixed-pattern noise were reduced. A vision chip with a 160×120 pixel array was fabricated using a 0.35 μm double-poly four-metal CMOS technology, and its operation was experimentally investigated.

  6. The prediction of AE, ap, and Dst at time lags between 0 and 30 hours

    Science.gov (United States)

    Smart, D. F.; Garrett, H. B.; Shea, M. A.

    1979-01-01

    The magnetic activity indexes AE, ap, and Dst are correlated with approximately 35,000 hours of interplanetary plasma and magnetic field measurements acquired near the Earth (assembled by NASA/NSSDC into a composite data sat). Lag times between the indexes and solar wind parameters ranged from 0 to 30 hours. Correlations at lags less than 6 hours yield results in agreement with previous studies. At greater lags, the correlation coefficients between the solar wind parameters and AE and ap approach these parameters' autocorrelation (persistence) values. For Dst the correlation with solar wind parameters is lower than that with AE and ap in the 3 to 4 hour lag range whereas the autocorrelation of Dst is significantly higher over the entire 0 to 30 hour lag range. The implications of these differences between AE, ap, and Dst are discussed in terms of persistence of solar wind structure.

  7. An Analysis of Media’s Role: Case Study of Army Public School (APS Peshawar Attack

    Directory of Open Access Journals (Sweden)

    Qureshi Rameesha

    2016-12-01

    Full Text Available The study aimed at analyzing the role of media during and after terrorist attacks by examining the media handling of APS Peshawar attack. The sample consisted of males and females selected on convenience basis from universities of Rawalpindi and Islamabad. It was hypothesized that (1 Extensive media coverage of terrorist attacks leads to greater publicity/recognition of terrorist groups (2 Media coverage of APS Peshawar attack increased fear and anxiety in public (3 Positive media handling/coverage of APS Peshawar attack led to public solidarity and peace. The results indicate that i Media coverage of terrorist attacks does help terrorist groups to gain publicity and recognition amongst public ii Media coverage of Aps Peshawar attack did not increase fear/anxiety in fact it directed the Pakistani nation towards public solidarity and peace.

  8. 76 FR 73720 - Knowledge and Abilities Catalog for Nuclear Power Plant Operators: Westinghouse AP1000...

    Science.gov (United States)

    2011-11-29

    ... From the Federal Register Online via the Government Publishing Office NUCLEAR REGULATORY COMMISSION Knowledge and Abilities Catalog for Nuclear Power Plant Operators: Westinghouse AP1000 Pressurized..., NUREG-2103, Revision 0, ``Knowledge and Abilities Catalog for Nuclear Power Plant...

  9. The prediction of AE, ap, and Dst at time lags between 0 and 30 hours

    Science.gov (United States)

    Smart, D. F.; Garrett, H. B.; Shea, M. A.

    1979-01-01

    The magnetic activity indexes AE, ap, and Dst are correlated with approximately 35,000 hours of interplanetary plasma and magnetic field measurements acquired near the Earth (assembled by NASA/NSSDC into a composite data sat). Lag times between the indexes and solar wind parameters ranged from 0 to 30 hours. Correlations at lags less than 6 hours yield results in agreement with previous studies. At greater lags, the correlation coefficients between the solar wind parameters and AE and ap approach these parameters' autocorrelation (persistence) values. For Dst the correlation with solar wind parameters is lower than that with AE and ap in the 3 to 4 hour lag range whereas the autocorrelation of Dst is significantly higher over the entire 0 to 30 hour lag range. The implications of these differences between AE, ap, and Dst are discussed in terms of persistence of solar wind structure.

  10. Determination of Heats of Fusion: Using Differential Scanning Calorimetry for the AP Chemistry Courses.

    Science.gov (United States)

    Temme, Susan M.

    1995-01-01

    Describes an exercise designed to be used in an Advanced Placement (AP) chemistry course to accompany the study of thermodynamics. Uses Differential Scanning Calorimetry in teaching the concepts of thermochemistry and thermodynamics. (JRH)

  11. Effects of podocin transfection on CD2AP distribution in HEK293 cells

    Institute of Scientific and Technical Information of China (English)

    Yugen SHA; Songming HUANG; Aihua ZHANG; Fei ZHAO; Ronghua CHEN

    2008-01-01

    The aim of this paper is to construct a podocin fluorescence expression vector and observe the effects of podocin transfection on CD2AP distribution in HEK293 cells. The pGEMT-easy vector containing the full-length cDNA encoding human podocin was cloned and digested with BamHI and XhoI. The digested full-length podocin was subcloned into pEGFP-C2. The constructed plas-mids were transfected into HEK293 cells and its effects on CD2AP distribution were observed by immunofluo-rescence. The pEGFP-NPHS2 expression vector was successfully constructed and podocin exclusively located on the HEK293 cell membrane. After podocin transfec-tion, CD2AP redistributed from the perinucleus to the cytoplasm in HEK293 cells. It can be concluded that podocin can recruit CD2AP to redistribute from the perinucleus to the cytoplasm in HEK293 cells.

  12. Mapping of the molecular determinants involved in the interaction between eps15 and AP-2

    DEFF Research Database (Denmark)

    Iannolo, G; Salcini, A E; Gaidarov, I

    1997-01-01

    domain, located in its NH2-terminal region. In addition, a proline-rich region, located in the COOH-terminal portion of eps15, can bind to the Src homology 3 domain of the crk proto-oncogene product in vitro. Recently, coimmunoprecipitation between eps15 and AP-2, a major component of coated pits......, was reported. Here, we characterize the molecular determinants of the eps15/AP-2 interaction. The AP-2 binding region of eps15 is localized in its COOH-terminal region and spans approximately 80 amino acids. At least three molecular determinants, located at residues 650-660, 680-690, and 720-730, are involved...... in the binding. AP-2 binds to eps15 through its alpha subunit (alpha-adaptin); in particular, the COOH-terminal region of alpha-adaptin, the so-called alpha-ear, contains the eps15 binding region....

  13. A self consistent chemically stratified atmosphere model for the roAp star 10 Aquilae

    CERN Document Server

    Nesvacil, Nicole; Ryabchikova, Tanya A; Kochukhov, Oleg; Akberov, Artur; Weiss, Werner W

    2012-01-01

    Context: Chemically peculiar A type (Ap) stars are a subgroup of the CP2 stars which exhibit anomalous overabundances of numerous elements, e.g. Fe, Cr, Sr and rare earth elements. The pulsating subgroup of the Ap stars, the roAp stars, present ideal laboratories to observe and model pulsational signatures as well as the interplay of the pulsations with strong magnetic fields and vertical abundance gradients. Aims: Based on high resolution spectroscopic observations and observed stellar energy distributions we construct a self consistent model atmosphere, that accounts for modulations of the temperature-pressure structure caused by vertical abundance gradients, for the roAp star 10 Aquilae (HD 176232). We demonstrate that such an analysis can be used to determine precisely the fundamental atmospheric parameters required for pulsation modelling. Methods: Average abundances were derived for 56 species. For Mg, Si, Ca, Cr, Fe, Co, Sr, Pr, and Nd vertical stratification profiles were empirically derived using the...

  14. Karakterisasi ekstrak kasar fitase termofilik dari bakteri kawah Ijen Banyuwangi, isolat AP-17

    Directory of Open Access Journals (Sweden)

    Aline Puspita Kusumadjaja

    2010-12-01

    Full Text Available Crude thermophilic phytase was produced by isolate AP-17 that has been isolated from Ijen Crater Banyuwangi. Based on Gram test, isolate AP-17 was gram positive spore forming rod shape bacteria so that it was identified as Bacillus sp. AP-17. Crude thermophilic phytase isolated from Bacillus sp. AP-17 had the optimum temperature at 75 ° C with activity of 0.1413 U/ml, and its optimum pH was at pH 6 with activity of 0.0875 U/ml. The enzyme was stable when heated at 75 ° C for three hours and still had 90% activity when it was exposed at pH 5 €“8, optimum temperature, for one hour.

  15. Genomic and transcriptomic analysis of the AP2/ERF superfamily in Vitis vinifera

    National Research Council Canada - National Science Library

    Licausi, Francesco; Giorgi, Federico M; Zenoni, Sara; Osti, Fabio; Pezzotti, Mario; Perata, Pierdomenico

    2010-01-01

    The AP2/ERF protein family contains transcription factors that play a crucial role in plant growth and development and in response to biotic and abiotic stress conditions in plants. Grapevine (Vitis vinifera...

  16. Determination of Heats of Fusion: Using Differential Scanning Calorimetry for the AP Chemistry Courses.

    Science.gov (United States)

    Temme, Susan M.

    1995-01-01

    Describes an exercise designed to be used in an Advanced Placement (AP) chemistry course to accompany the study of thermodynamics. Uses Differential Scanning Calorimetry in teaching the concepts of thermochemistry and thermodynamics. (JRH)

  17. Novel players in the AP2-miR172 regulatory network for common bean nodulation.

    Science.gov (United States)

    Íñiguez, Luis P; Nova-Franco, Bárbara; Hernández, Georgina

    2015-01-01

    The intricate regulatory network for floral organogenesis in plants that includes AP2/ERF, SPL and AGL transcription factors, miR172 and miR156 along with other components is well documented, though its complexity and size keep increasing. The miR172/AP2 node was recently proposed as essential regulator in the legume-rhizobia nitrogen-fixing symbiosis. Research from our group contributed to demonstrate the control of common bean (Phaseolus vulgaris) nodulation by miR172c/AP2-1, however no other components of such regulatory network have been reported. Here we propose AGLs as new protagonists in the regulation of common bean nodulation and discuss the relevance of future deeper analysis of the complex AP2 regulatory network for nodule organogenesis in legumes.

  18. Magnetic Measurements of Storage Ring Magnets for the APS Upgrade Project

    Energy Technology Data Exchange (ETDEWEB)

    Doose, C.; Dejus, R.; Jaski, M.; Jansma, W.; Collins, J.; Donnelly, A.; Liu, J.; Cease, H.; Decker, G.; Jain, A.; DiMarco, J.

    2017-06-01

    Extensive prototyping of storage ring magnets is ongoing at the Advanced Photon Source (APS) in support of the APS Multi-Bend Achromat (MBA) upgrade project (APS-U) [1]. As part of the R&D activities four quadrupole magnets with slightly different geometries and pole tip materials, and one sextupole magnet with vanadium permendur (VP) pole tips were designed, built and tested. Magnets were measured individually using a rotating coil and a Hall probe for detailed mapping of the magnetic field. Magnets were then assembled and aligned relative to each other on a steel support plate and concrete plinth using precision machined surfaces to gain experience with the alignment method chosen for the APS-U storage ring magnets. The required alignment of magnets on a common support structure is 30 μm rms. Measurements of magnetic field quality, strength and magnet alignment after subjecting the magnets and assemblies to different tests are presented.

  19. Biological Databases

    Directory of Open Access Journals (Sweden)

    Kaviena Baskaran

    2013-12-01

    Full Text Available Biology has entered a new era in distributing information based on database and this collection of database become primary in publishing information. This data publishing is done through Internet Gopher where information resources easy and affordable offered by powerful research tools. The more important thing now is the development of high quality and professionally operated electronic data publishing sites. To enhance the service and appropriate editorial and policies for electronic data publishing has been established and editors of article shoulder the responsibility.

  20. Struktura i svojstva plazma sprej Aps - Nikal - Aluminid prevlake

    Directory of Open Access Journals (Sweden)

    Mihailo Rade Mrdak

    2013-06-01

    Full Text Available Cilj ovog istraživanja je da se optimizacijom parametara deponovanja dobiju optimalne strukturne i mehaničke karakteristike Ni20Al slojeva, koji će se primeniti na delu za radarsku stanicu. Prah je deponovan atmosferskim plazma sprej (APS postupkom sa plazma sprej (PS odstojanjem 80, 90 i 100 mm. Prevlaka sa najboljim karakteristikama deponovana je na utvrđivaču  turbo- gasnog motora GTD 2PV8 za radarsku stanicu, da bi se smanjio uticaj oksidacije  i vibracija na habanje na 450–500°C. Procene Ni20Al slojeva prevlake urađene su na osnovu njihovih mikrostruktura, mikrotvrdoća i zatezne čvrstoće. Najbolju strukturu i mehaničke  karakteristike pokazali su slojevi deponovani sa plazma sprej odstojanjem od 80 mm. Morfologija površine deponovane prevlake i morfologija loma najboljih slojeva ispitana je na SEM-u (skening elektronskom mikroskopu. Mikrostruktura slojeva deponovanih prevlaka ispitana je na svetlosnom mikroskopu. Mikrostrukturna analiza deponovanih slojevima urađena je u skladu sa standardom Pratt-Whitney. Procena mehaničkih karakteristika slojeva urađena je ispitivanjem mikrotvrdoće metodom HV0.3 i čvrstoće spoja ispitivanjem na zatezanje. Istraživanja su pokazala da plazma sprej odstojanje bitno utiče na mehaničke osobine i mikrostrukture prevlaka. Efekat primene Ni20Al prevlake ispitan je na utvrđivaču  turbogasnog motora GTD 2PV8 na radarskoj stanici.   Uvod   Kompozitni nikal-aluminid prah Ni20Al razvijen je za potrebe vazduhoplovne industrije. Važna karakteristika je dobro deponovanje praha plazma sprejom na atmosferskom pritisku (APS. Ovaj materijal ima dobru otpornost na oksidaciju,  koroziju i eroziju. Materijal se može proizvoditi kao legura ili kao  kompozitni prah. Najvažnija karakteristika nikal-aluminida je velika čvrstoća i žilavost  sa odličnom otpornošću na visoke temperature. NiAl prevlake deponovane plazma sprejom našle su široku primenu za zaštitu delova koji su izlo

  1. Comparing the Floating Point Systems, Inc. AP-190L to representative scientific computers: some benchmark results

    Energy Technology Data Exchange (ETDEWEB)

    Brengle, T.A.; Maron, N.

    1980-03-27

    Results are presented of comparative timing tests made by running a typical FORTRAN physics simulation code on the following machines: DEC PDP-10 with KI processor; DEC PDP-10, KI processor, and FPS AP-190L; CDC 7600; and CRAY-1. Factors such as DMA overhead, code size for the AP-190L, and the relative utilization of floating point functional units for the different machines are discussed. 1 table.

  2. Catalysis of Nanometer α-Fe2O3 on the Thermal Decomposition of AP

    Institute of Scientific and Technical Information of China (English)

    YANG Yi; LI Feng-sheng; LIU Hong-ying; JIANG Wei; CUI Ping

    2005-01-01

    Nanometer α-Fe2O3 catalysts were prepared by hydrolyzation in high temperature. Three kinds of precipitators, NaOH, (NH4)2CO3 and urea were used to compare the effect in the process of hydrolyzation. Nanometer sizer, transmission electron microscopy (TEM) and X-ray diffraction (XRD) were employed to test the profiles and diameters of the product particles. The test results indicate that the production is nanometer α-Fe2O3 with narrow particle size distribution (PSD) and good dispersibility. The catalysts are mixed with ammonia perchlorate (AP) in 1.0 wt.%. And the composite particles of catalysts with AP are prepared using a new solvent-nonsolvent method. Differential thermal analyzer (DTA) is employed to analysis the thermal decomposition of the composite particles and pure AP sample. The results imply that the thermal decomposition curve peaks of the samples in which nanometer α-Fe2O3 catalysts are added appear comparatively more ahead than that of pure AP sample. Among these mixtures added nanometer material, the smaller the particle diameter of catalyst is, the more ahead the thermal decomposition curve peaks of AP appear. The high and low temperature thermal decomposition curve peaks of AP mixed with the catalyst deposed by urea are more ahead of 77.8℃ and 9.7℃ than that of pure AP, respectively. The mechanism of the catalyst deposed by urea with smaller diameter and the distinct catalysis of the particles on the thermal decomposition of AP are discussed.

  3. Synaptic and genomic responses to JNK and AP-1 signaling in Drosophila neurons

    Directory of Open Access Journals (Sweden)

    Bohmann Dirk

    2005-06-01

    Full Text Available Abstract Background The transcription factor AP-1 positively controls synaptic plasticity at the Drosophila neuromuscular junction. Although in motor neurons, JNK has been shown to activate AP-1, a positive regulator of growth and strength at the larval NMJ, the consequences of JNK activation are poorly studied. In addition, the downstream transcriptional targets of JNK and AP-1 signaling in the Drosophila nervous system have yet to be identified. Here, we further investigated the role of JNK signaling at this model synapse employing an activated form of JNK-kinase; and using Serial Analysis of Gene Expression and oligonucleotide microarrays, searched for candidate early targets of JNK or AP-1 dependent transcription in neurons. Results Temporally-controlled JNK induction in postembryonic motor neurons triggers synaptic growth at the NMJ indicating a role in developmental plasticity rather than synaptogenesis. An unexpected observation that JNK activation also causes a reduction in transmitter release is inconsistent with JNK functioning solely through AP-1 and suggests an additional, yet-unidentified pathway for JNK signaling in motor neurons. SAGE profiling of mRNA expression helps define the neural transcriptome in Drosophila. Though many putative AP-1 and JNK target genes arose from the genomic screens, few were confirmed in subsequent validation experiments. One potentially important neuronal AP-1 target discovered, CG6044, was previously implicated in olfactory associative memory. In addition, 5 mRNAs regulated by RU486, a steroid used to trigger conditional gene expression were identified. Conclusion This study demonstrates a novel role for JNK signaling at the larval neuromuscular junction and provides a quantitative profile of gene transcription in Drosophila neurons. While identifying potential JNK/AP-1 targets it reveals the limitations of genome-wide analyses using complex tissues like the whole brain.

  4. Building a world-class A/P function: how UPMC went paperless.

    Science.gov (United States)

    DeLuca, Michael; Smith, Corey

    2010-03-01

    UPMC engaged people, processes, and technology to move its A/P function from a highly manual, paper-based operation to a completely automated process. UPMC's CFO hired a chief supply chain officer to develop a strategic plan, and UPMC named a value analysis team to gain clinician buy-in. UPMC automated A/P by enabling receipt of electronic invoices. UPMC streamlined its processes for invoices.

  5. 基于城域网的瘦AP+AC的WLAN网络构建

    Institute of Scientific and Technical Information of China (English)

    曹炳健; 钟明东

    2011-01-01

    本文主要介绍了基于城域网的瘦AP+AC架构的WLAN网络构建,分析了瘦AP+AC的网络架构中,AC部署方式的不同对城域网的影响,并进一步并对AC的容量规划、AC的备份机制等问题进行了探讨.

  6. Chemogenomic identification of Ref-1/AP-1 as a therapeutic target for asthma

    OpenAIRE

    Nguyen, Cu; Teo, Jia-Ling; Matsuda, Akihisa; Eguchi, Masakatsu; Emil Y Chi; William R Henderson; Kahn, Michael

    2003-01-01

    Asthma is characterized by an oxidant/antioxidant imbalance in the lungs leading to activation of redox-sensitive transcription factors, nuclear factor κB (NF-κB), and activator protein-1 (AP-1). To develop therapeutic strategies for asthma, we used a chemogenomics approach to screen for small molecule inhibitor(s) of AP-1 transcription. We developed a β-strand mimetic template that acts as a reversible inhibitor (pseudosubstrate) of redox proteins. This template incorporates an enedione moie...

  7. Structural Determinants of DNA Binding by a P. falciparum ApiAP2 Transcriptional Regulator

    Energy Technology Data Exchange (ETDEWEB)

    Lindner, Scott E.; De Silva, Erandi K.; Keck, James L.; Llinás, Manuel (Princeton); (UW-MED)

    2010-11-05

    Putative transcription factors have only recently been identified in the Plasmodium spp., with the major family of regulators comprising the Apicomplexan Apetala2 (AP2) proteins. To better understand the DNA-binding mechanisms of these transcriptional regulators, we characterized the structure and in vitro function of an AP2 DNA-binding domain from a prototypical Apicomplexan AP2 protein, PF14{_}0633 from Plasmodium falciparum. The X-ray crystal structure of the PF14{_}0633 AP2 domain bound to DNA reveals a {beta}-sheet fold that binds the DNA major groove through base-specific and backbone contacts; a prominent {alpha}-helix supports the {beta}-sheet structure. Substitution of predicted DNA-binding residues with alanine weakened or eliminated DNA binding in solution. In contrast to plant AP2 domains, the PF14{_}0633 AP2 domain dimerizes upon binding to DNA through a domain-swapping mechanism in which the {alpha}-helices of the AP2 domains pack against the {beta}-sheets of the dimer mates. DNA-induced dimerization of PF14{_}0633 may be important for tethering two distal DNA loci together in the nucleus and/or for inducing functional rearrangements of its domains to facilitate transcriptional regulation. Consistent with a multisite binding mode, at least two copies of the consensus sequence recognized by PF14{_}0633 are present upstream of a previously identified group of sporozoite-stage genes. Taken together, these findings illustrate how Plasmodium has adapted the AP2 DNA-binding domain for genome-wide transcriptional regulation.

  8. Antiphospholipid syndrome (APS) revisited: Would migraine headaches be included in future classification criteria?

    Science.gov (United States)

    Noureldine, Mohammad Hassan A; Haydar, Ali A; Berjawi, Ahmad; Elnawar, Rody; Sweid, Ahmad; Khamashta, Munther A; Hughes, Graham R V; Uthman, Imad

    2016-07-16

    Headaches have been extensively reported in Antiphospholipid syndrome (APS)/Antiphospholipid antibodies (aPL)-positive patients. The aim of this study was to highlight the prevalence of headaches among APS/aPL-positive patients and discuss its association with laboratory, clinical and imaging findings. We searched the literature through Google Scholar and PubMed for publications on the epidemiology, pathogenesis, laboratory, imaging and clinical findings, and management of headaches in APS/aPL-positive patients. The following keywords were used: Antiphospholipid, Hughes syndrome, anticardiolipin, lupus anticoagulant, anti-β2 glycoprotein I, headache, migraine, tension, and cluster. All reports published between 1969 and 2015 were included. Migraine is the most commonly reported type of headache in APS/aPL-positive patients. Thrombotic and platelet dysfunction hypotheses have been studied to uncover the pathogenic role of aPL in the development of headaches. Several studies are reporting higher levels of aPL in primary and secondary APS migraineurs, but only few reached statistical significance. Migraine patients without clinical signs/symptoms of cerebral infarction rarely show positive imaging findings. Digital subtraction angiography shows promise in demonstrating small vascular lesions otherwise not detected on computed tomography, magnetic resonance imaging, or cerebral angiograms. Although it may be solitary and harmless in many cases, the deleterious effect of migraine on the quality of life of APS patients prompts rapid diagnosis and proper management. An anticoagulation trial is advisable in APS patients with migraine as many cases of severe, refractory migraine resolved with anticoagulation therapy. The profile of migraine headaches discussed in this study permits its candidacy for inclusion in future APS classification criteria.

  9. Biological biomaterials

    Energy Technology Data Exchange (ETDEWEB)

    Jorge-Herrero, E. [Servicio de Cirugia Experimental. Clinica Puerta de Hierro, Madrid (Spain)

    1997-05-01

    There are a number of situations in which substances of biological origin are employed as biomaterials. Most of them are macromolecules derived from isolated connective tissue or the connective tissue itself in membrane form, in both cases, the tissue can be used in its natural form or be chemically treated. In other cases, certain blood vessels can be chemically pretreated and used as vascular prostheses. Proteins such as albumin, collagen and fibrinogen are employed to coat vascular prostheses. Certain polysaccharides have also been tested for use in controlled drug release systems. Likewise, a number of tissues, such as dura mater, bovine pericardium, procine valves and human valves, are used in the preparation of cardiac prostheses. We also use veins from animals or humans in arterial replacement. In none of these cases are the tissues employed dissimilar to the native tissues as they have been chemically modified, becoming a new bio material with different physical and biochemical properties. In short, we find that natural products are being utilized as biomaterials and must be considered as such; thus, it is necessary to study both their chemicobiological and physicomechanical properties. In the present report, we review the current applications, problems and future prospects of some of these biological biomaterials. (Author) 84 refs.

  10. Expression patterns of LmAP2L1 and LmAP2L2 encoding two-APETALA2 domain proteins during somatic embryogenesis and germination of hybrid larch (Larix x marschlinsii).

    Science.gov (United States)

    Guillaumot, Damien; Lelu-Walter, Marie-Anne; Germot, Agnès; Meytraud, Fanny; Gastinel, Louis; Riou-Khamlichi, Catherine

    2008-06-16

    Two APETALA2 domain transcription factors were characterized first in angiosperms, and, recently, in several gymnosperms. These proteins are involved in several processes, from flowering to embryogenesis in Arabidopsis thaliana. We extrapolated this result to hybrid larch (Larixxmarschlinsii Coaz) resulting from a cross between European (Larix decidua) and Japanese (Larix kaempferi) larches. Somatic embryogenesis is well described and controlled for this Pinaceae. We characterized two-AP2 domain genes: LmAP2L1 and LmAP2L2. Phylogenetic analysis confirmed that LmAP2L1 and LmAP2L2 were orthologous to Norway spruce PaAP2L1 and PaAP2L2 and that L1 forms appeared to be specific to Pinaceae. RT-PCR analysis showed that larch APETALA2 was differentially expressed during late somatic embryogenesis and during the first steps of germination. Whereas LmAP2L2 was constitutively expressed during this process, LmAP2L1 expression appeared only during late somatic embryogenesis, when embryos were able to germinate. Further, LmAP2L1 appeared to be the preferentially expressed form during embryo germination. Thus, LmAP2L1 seems to be a valuable molecular marker for hybrid larch late somatic embryogenesis and could play a role during post-embryonic development.

  11. Simulation of the Westinghouse AP1000 Response to SBLOCA Using RELAP/SCDAPSIM

    Directory of Open Access Journals (Sweden)

    Ayah Elshahat

    2014-01-01

    Full Text Available Great interest is given now to advanced nuclear reactors especially those using passive safety components. The Westinghouse AP1000 Advanced Passive pressurized water reactor (PWR is an 1117 MWe PWR designed to achieve a high safety and performance record. The AP1000 safety system uses natural driving forces, such as pressurized gas, gravity flow, natural circulation flow, and convection. In this paper, the safety performance of the AP1000 during a small break loss of coolant accident (SBLOCA is investigated. This was done by modelling the AP1000 and the passive safety systems employed using RELAP/SCDAPSIM code. RELAP/SCDAPSIM is designed to describe the overall reactor coolant system (RCS thermal hydraulic response and core behaviour under normal operating conditions or under design basis or severe accident conditions. Passive safety components in the AP1000 showed a clear improvement in accident mitigation. It was found that RELAP/SCDAPSIM is capable of modelling a LOCA in an AP1000 and it enables the investigation of each safety system component response separately during the accident. The model is also capable of simulating natural circulation and other relevant phenomena. The results of the model were compared to that of the NOTRUMP code and found to be in a good agreement.

  12. Comparative incidence of pregnancy outcomes in treated obstetric antiphospholipid syndrome: the NOH-APS observational study.

    Science.gov (United States)

    Bouvier, Sylvie; Cochery-Nouvellon, Eva; Lavigne-Lissalde, Géraldine; Mercier, Erick; Marchetti, Tess; Balducchi, Jean-Pierre; Marès, Pierre; Gris, Jean-Christophe

    2014-01-16

    The incidence of pregnancy outcomes for women with the purely obstetric form of antiphospholipid syndrome (APS) treated with prophylactic low-molecular-weight heparin (LMWH) plus low-dose aspirin (LDA) has not been documented. We observed women without a history of thrombosis who had experienced 3 consecutive spontaneous abortions before the 10th week of gestation or 1 fetal loss at or beyond the 10th week. We compared the frequencies of complications during new pregnancies between treated women with APS (n = 513; LMWH + LDA) and women negative for antiphospholipid antibodies as controls (n = 791; no treatment). Among APS women, prior fetal loss was a risk factor for fetal loss, preeclampsia (PE), premature birth, and the occurrence of any placenta-mediated complication. Being positive for anticardiolipin immunoglobulin M antibodies was a risk factor for any placenta-mediated complication. Among women with a history of recurrent abortion, APS women were at a higher risk than other women of PE, placenta-mediated complications, and neonatal mortality. Among women with prior fetal loss, LMWH + LDA-treated APS women had lower pregnancy loss rates but higher PE rates than other women. Improved therapies, in particular better prophylaxis of late pregnancy complications, are urgently needed for obstetric APS and should be evaluated according to the type of pregnancy loss.

  13. CD2v Interacts with Adaptor Protein AP-1 during African Swine Fever Infection.

    Directory of Open Access Journals (Sweden)

    Daniel Pérez-Núñez

    Full Text Available African swine fever virus (ASFV CD2v protein is believed to be involved in virulence enhancement, viral hemadsorption, and pathogenesis, although the molecular mechanisms of the function of this viral protein are still not fully understood. Here we describe that CD2v localized around viral factories during ASFV infection, suggesting a role in the generation and/or dynamics of these viral structures and hence in disturbing cellular traffic. We show that CD2v targeted the regulatory trans-Golgi network (TGN protein complex AP-1, a key element in cellular traffic. This interaction was disrupted by brefeldin A even though the location of CD2v around the viral factory remained unchanged. CD2v-AP-1 binding was independent of CD2v glycosylation and occurred on the carboxy-terminal part of CD2v, where a canonical di-Leu motif previously reported to mediate AP-1 binding in eukaryotic cells, was identified. This motif was shown to be functionally interchangeable with the di-Leu motif present in HIV-Nef protein in an AP-1 binding assay. However, we demonstrated that it was not involved either in CD2v cellular distribution or in CD2v-AP-1 binding. Taken together, these findings shed light on CD2v function during ASFV infection by identifying AP-1 as a cellular factor targeted by CD2v and hence elucidate the cellular pathways used by the virus to enhance infectivity.

  14. CMOS-APS Detectors for Solar Physics: Lessons Learned during the SWAP Preflight Calibration

    Science.gov (United States)

    de Groof, A.; Berghmans, D.; Nicula, B.; Halain, J.-P.; Defise, J.-M.; Thibert, T.; Schühle, U.

    2008-05-01

    CMOS-APS imaging detectors open new opportunities for remote sensing in solar physics beyond what classical CCDs can provide, offering far less power consumption, simpler electronics, better radiation hardness, and the possibility of avoiding a mechanical shutter. The SWAP telescope onboard the PROBA2 technology demonstration satellite of the European Space Agency will be the first actual implementation of a CMOS-APS detector for solar physics in orbit. One of the goals of the SWAP project is precisely to acquire experience with the CMOS-APS technology in a real-live space science context. Such a precursor mission is essential in the preparation of missions such as Solar Orbiter where the extra CMOS-APS functionalities will be hard requirements. The current paper concentrates on specific CMOS-APS issues that were identified during the SWAP preflight calibration measurements. We will discuss the different readout possibilities that the CMOS-APS detector of SWAP provides and their associated pros and cons. In particular we describe the “image lag” effect, which results in a contamination of each image with a remnant of the previous image. We have characterised this effect for the specific SWAP implementation and we conclude with a strategy on how to successfully circumvent the problem and actually take benefit of it for solar monitoring.

  15. Effects of aluminum and iron nanoparticle additives on composite AP/HTPB solid propellant regression rate

    Science.gov (United States)

    Styborski, Jeremy A.

    This project was started in the interest of supplementing existing data on additives to composite solid propellants. The study on the addition of iron and aluminum nanoparticles to composite AP/HTPB propellants was conducted at the Combustion and Energy Systems Laboratory at RPI in the new strand-burner experiment setup. For this study, a large literature review was conducted on history of solid propellant combustion modeling and the empirical results of tests on binders, plasticizers, AP particle size, and additives. The study focused on the addition of nano-scale aluminum and iron in small concentrations to AP/HTPB solid propellants with an average AP particle size of 200 microns. Replacing 1% of the propellant's AP with 40-60 nm aluminum particles produced no change in combustive behavior. The addition of 1% 60-80 nm iron particles produced a significant increase in burn rate, although the increase was lesser at higher pressures. These results are summarized in Table 2. The increase in the burn rate at all pressures due to the addition of iron nanoparticles warranted further study on the effect of concentration of iron. Tests conducted at 10 atm showed that the mean regression rate varied with iron concentration, peaking at 1% and 3%. Regardless of the iron concentration, the regression rate was higher than the baseline AP/HTPB propellants. These results are summarized in Table 3.

  16. Mutation screening of NOS1AP gene in a large sample of psychiatric patients and controls

    Directory of Open Access Journals (Sweden)

    Nygren Gudrun

    2010-07-01

    Full Text Available Abstract Background The gene encoding carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase (NOS1AP is located on chromosome 1q23.3, a candidate region for schizophrenia, autism spectrum disorders (ASD and obsessive-compulsive disorder (OCD. Previous genetic and functional studies explored the role of NOS1AP in these psychiatric conditions, but only a limited number explored the sequence variability of NOS1AP. Methods We analyzed the coding sequence of NOS1AP in a large population (n = 280, including patients with schizophrenia (n = 72, ASD (n = 81 or OCD (n = 34, and in healthy volunteers controlled for the absence of personal or familial history of psychiatric disorders (n = 93. Results Two non-synonymous variations, V37I and D423N were identified in two families, one with two siblings with OCD and the other with two brothers with ASD. These rare variations apparently segregate with the presence of psychiatric conditions. Conclusions Coding variations of NOS1AP are relatively rare in patients and controls. Nevertheless, we report the first non-synonymous variations within the human NOS1AP gene that warrant further genetic and functional investigations to ascertain their roles in the susceptibility to psychiatric disorders.

  17. Comparative Advantages of AP1000 Passive Safety System%AP1000核电站非能动安全系统的比较优势

    Institute of Scientific and Technical Information of China (English)

    叶成; 郑明光; 韩旭; 陈松

    2012-01-01

    With the increasing need of nuclear power, almost all countries who want to build new nuclear power plant take the generation HI technology as their first choice. The main reason is that the safety of generation M technology has been improved greatly than generation II and II + technology. The passive safety technology is introduced by AP1000 and it is one of the best in generation m technologies. The representative passive containment cooling system of AP1000 and containment spray system of generation II nuclear power plant were compared in the paper and analyzed using probabilistic safety assessment method. The reasons of passive safety system possessing comparative advantages were obtained by the concrete calculation.%面对日益增长的核电发展需求,几乎所有的国家都把新的核电项目定位于第Ⅲ代核电技术,其中一个重要原因就是因为第Ⅲ代核电技术的安全性相对于第Ⅱ代和Ⅱ+核电技术的安全性有了很大提高.第Ⅲ代核电技术中的AP1000采用非能动安全技术,极大提高了安全性能指标.对AP1000与第Ⅱ代核电技术中具有代表性的安全系统,即AP1000中的非能动安全壳冷却系统(PCS)和第Ⅱ代核电中的喷淋系统(SCS),进行了比较,从概率安全评价(PSA)的角度对它们进行分析,通过具体计算得出了非能动安全系统具有比较优势的原因.

  18. Local Arp2/3-dependent actin assembly modulates applied traction force during apCAM adhesion site maturation.

    Science.gov (United States)

    Buck, Kenneth B; Schaefer, Andrew W; Schoonderwoert, Vincent T; Creamer, Matthew S; Dufresne, Eric R; Forscher, Paul

    2017-01-01

    Homophilic binding of immunoglobulin superfamily molecules such as the Aplysia cell adhesion molecule (apCAM) leads to actin filament assembly near nascent adhesion sites. Such actin assembly can generate significant localized forces that have not been characterized in the larger context of axon growth and guidance. We used apCAM-coated bead substrates applied to the surface of neuronal growth cones to characterize the development of forces evoked by varying stiffness of mechanical restraint. Unrestrained bead propulsion matched or exceeded rates of retrograde network flow and was dependent on Arp2/3 complex activity. Analysis of growth cone forces applied to beads at low stiffness of restraint revealed switching between two states: frictional coupling to retrograde flow and Arp2/3-dependent propulsion. Stiff mechanical restraint led to formation of an extensive actin cup matching the geometric profile of the bead target and forward growth cone translocation; pharmacological inhibition of the Arp2/3 complex or Rac attenuated F-actin assembly near bead binding sites, decreased the efficacy of growth responses, and blocked accumulation of signaling molecules associated with nascent adhesions. These studies introduce a new model for regulation of traction force in which local actin assembly forces buffer nascent adhesion sites from the mechanical effects of retrograde flow. © 2017 Buck et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

  19. Creating biological nanomaterials using synthetic biology

    Science.gov (United States)

    Rice, MaryJoe K.; Ruder, Warren C.

    2014-02-01

    Synthetic biology is a new discipline that combines science and engineering approaches to precisely control biological networks. These signaling networks are especially important in fields such as biomedicine and biochemical engineering. Additionally, biological networks can also be critical to the production of naturally occurring biological nanomaterials, and as a result, synthetic biology holds tremendous potential in creating new materials. This review introduces the field of synthetic biology, discusses how biological systems naturally produce materials, and then presents examples and strategies for incorporating synthetic biology approaches in the development of new materials. In particular, strategies for using synthetic biology to produce both organic and inorganic nanomaterials are discussed. Ultimately, synthetic biology holds the potential to dramatically impact biological materials science with significant potential applications in medical systems.

  20. Insect Cell Glycosylation and Its Impact on the Functionality of a Recombinant Intracrystalline Nacre Protein, AP24.

    Science.gov (United States)

    Chang, Eric P; Perovic, Iva; Rao, Ashit; Cölfen, Helmut; Evans, John Spencer

    2016-02-23

    The impacts of glycosylation on biomineralization protein function are largely unknown. This is certainly true for the mollusk shell, where glycosylated intracrystalline proteins such as AP24 (Haliotis rufescens) exist but their functions and the role of glycosylation remain elusive. To assess the effect of glycosylation on protein function, we expressed two recombinant variants of AP24: an unglycosylated bacteria-expressed version (rAP24N) and a glycosylated insect cell-expressed version (rAP24G). Our findings indicate that rAP24G is expressed as a single polypeptide containing variations in glycosylation that create microheterogeneity in rAP24G molecular masses. These post-translational modifications incorporate O- and N-glycans and anionic monosialylated and bisialylated, and monosulfated and bisulfated monosaccharides on the protein molecules. AFM and DLS experiments confirm that both rAP24N and rAP24G aggregate to form protein phases, with rAP24N exhibiting a higher degree of aggregation, compared to rAP24G. With regard to functionality, we observe that both recombinant proteins exhibit similar behavior within in vitro calcium carbonate mineralization assays and potentiometric titrations. However, rAP24G modifies crystal growth directions and is a stronger nucleation inhibitor, whereas rAP24N exhibits higher mineral phase stabilization and nanoparticle containment. We believe that the post-translational addition of anionic groups (via sialylation and sulfation), along with modifications to the protein surface topology, may explain the changes in glycosylated rAP24G aggregation and mineralization behavior, relative to rAP24N.

  1. PEMETAAN DISTRIBUSI SUHU DAN DNBR PADA PERANGKAT BAHAN BAKAR AP1000-EU

    Directory of Open Access Journals (Sweden)

    Muh. Darwis Isnaini

    2015-04-01

    Full Text Available Telah dilakukan suatu pemetaan distribusi suhu dan DNBR minimum pada perangkat bahan bakar AP1000-EU. Untuk meningkatkan kemampuan SDM dalam memahami desain PLTN, diperlukan karakteristik desain yang lengkap. Dengan latar belakang bahwa, untuk mendapatkan karakteristik termohidrolika yang lengkap, maka tujuan penelitian ini melakukan pemetaan distribusi distribusi suhu meat bahan bakar, kelongsong, pendingin dan DNBR minimum serta fluks panas dari 1/8 perangkat bahan bakar AP1000-EU pada kondisi BOC daya penuh, xenon setimbang dan batang kendali terangkat dari teras. Dipilihnya objek desain AP1000-EU ini karena dokumen kontrol desain AP1000 buatan Westinghouse Amerika Serikat ini sedang dalam proses sertifikasi oleh badan regulasi Inggris (negara Eropa. Desain AP1000-EU mempunyai daya termal reaktor yang sama 3400 MWt, jumlah perangkat bahan bakar sama 157 buah, tekanan operasi 15,1 MPa. Perangkat bahan bakar AP1000-EU terdiri atas 264 rod bahan bakar dengan ukuran diameter sebesar 0,95 cm dengan panjang 426,72 cm. Perhitungan dilakukan dengan code COBRA-EN pada 1/8 perangkat bahan bakar posisi G-9 dengan faktor daya bervariasi dari 1,124 sampai 1,396, dan hasilnya dibuat peta distribusi. Dari hasil pemetaan menunjukkan bahwa suhu maksimum tengah meat sebesar 1032,95 oC jauh lebih rendah dibanding batas maksimum desain, dan DNBR minimum 4,395 jauh lebih besar dibanding batas minimum desain. Hasil pemetaan distribusi suhu, DNBR dan fluks panas pada kondisi operasi normal, daya penuh pada awal siklus, cukup untuk menjawab peta distribusi di akhir siklus bahan bakar. Kata kunci : Pemetaan, distribusi suhu, DNBR, AP1000-EU.   The mapping of DNBR and temperature distribution for EU-AP1000’s fuel assembly was carried out. The development of the human resources capability to understand the NPP design, requires comprehensive characteristic design. The background of this research, is that in order to find the comprehensive NPP characteristic design

  2. Biological Sciences and Bioelectromagnetics in Europe: Summary Report.

    Science.gov (United States)

    1985-08-26

    ankylosing spondylitis , osteoporosis, low levels of EM energy are capable of pseudoarthrosis, and nonunions can ap- eliciting a response from a...engineering, radiation physics , Early in 1984, I reported on the radiation biology, and bioelectromagnet- International Center for Genetic Engi- ice...biomaterials in are to be considered biomaterials. therapy . There are fewer roadblocks be- First, and most importantly, the materi- tween development and

  3. Comparison of a CCD and an APS for soft X-ray diffraction

    Science.gov (United States)

    Stewart, Graeme; Bates, R.; Blue, A.; Clark, A.; Dhesi, S. S.; Maneuski, D.; Marchal, J.; Steadman, P.; Tartoni, N.; Turchetta, R.

    2011-12-01

    We compare a new CMOS Active Pixel Sensor (APS) to a Princeton Instruments PIXIS-XO: 2048B Charge Coupled Device (CCD) with soft X-rays tested in a synchrotron beam line at the Diamond Light Source (DLS). Despite CCDs being established in the field of scientific imaging, APS are an innovative technology that offers advantages over CCDs. These include faster readout, higher operational temperature, in-pixel electronics for advanced image processing and reduced manufacturing cost. The APS employed was the Vanilla sensor designed by the MI3 collaboration and funded by an RCUK Basic technology grant. This sensor has 520 x 520 square pixels, of size 25 μm on each side. The sensor can operate at a full frame readout of up to 20 Hz. The sensor had been back-thinned, to the epitaxial layer. This was the first time that a back-thinned APS had been demonstrated at a beam line at DLS. In the synchrotron experiment soft X-rays with an energy of approximately 708 eV were used to produce a diffraction pattern from a permalloy sample. The pattern was imaged at a range of integration times with both sensors. The CCD had to be operated at a temperature of -55°C whereas the Vanilla was operated over a temperature range from 20°C to -10°C. We show that the APS detector can operate with frame rates up to two hundred times faster than the CCD, without excessive degradation of image quality. The signal to noise of the APS is shown to be the same as that of the CCD at identical integration times and the response is shown to be linear, with no charge blooming effects. The experiment has allowed a direct comparison of back thinned APS and CCDs in a real soft x-ray synchrotron experiment.

  4. Adaptor Protein CD2AP and L-type Lectin LMAN2 Regulate Exosome Cargo Protein Trafficking through the Golgi Complex.

    Science.gov (United States)

    Kwon, Sang-Ho; Oh, Sekyung; Nacke, Marisa; Mostov, Keith E; Lipschutz, Joshua H

    2016-12-02

    Exosomes, 40-150-nm extracellular vesicles, transport biological macromolecules that mediate intercellular communications. Although exosomes are known to originate from maturation of endosomes into multivesicular endosomes (also known as multivesicular bodies) with subsequent fusion of the multivesicular endosomes with the plasma membrane, it remains unclear how cargos are selected for exosomal release. Using an inducible expression system for the exosome cargo protein GPRC5B and following its trafficking trajectory, we show here that newly synthesized GPRC5B protein accumulates in the Golgi complex prior to its release into exosomes. The L-type lectin LMAN2 (also known as VIP36) appears to be specifically required for the accumulation of GPRC5B in the Golgi complex and restriction of GPRC5B transport along the exosomal pathway. This may occur due to interference with the adaptor protein GGA1-mediated trans Golgi network-to-endosome transport of GPRC5B. The adaptor protein CD2AP-mediated internalization following cell surface delivery appears to contribute to the Golgi accumulation of GPRC5B, possibly in parallel with biosynthetic/secretory trafficking from the endoplasmic reticulum. Our data thus reveal a Golgi-traversing pathway for exosomal release of the cargo protein GPRC5B in which CD2AP facilitates the entry and LMAN2 impedes the exit of the flux, respectively. © 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

  5. AP1000给水丧失事故定性分析%Qualitative Accident Analysis on Loss of Normal Feedwater for AP1000

    Institute of Scientific and Technical Information of China (English)

    李延凯; 林萌; 侯东; 李美琳; 杨燕华

    2012-01-01

    针对AP1000核电站,基于两流体最佳估算系统程序RELAP5建立热工水力模型,基于Matlab/Simulink软件及工业组态软件建立相关控制系统数学模型,用于对正常给水丧失事故的计算分析.建模数据主要参考AP1000 Design Control Document(AP1000 DCD),由于建模数据不够充分、详尽,模型不够精确,文中事故分析以定性分析为主.计算结果表明:RELAP5具备计算自然循环的能力,计算结果与DCD中正常给水丧失事故结果总体趋势基本一致,非能动余热排出系统(PRHRS)、堆芯补水箱(CMT)系统能够及时、有效地排出堆芯余热和堆芯衰变热,确保堆芯安全.PRHRS余热排出能力对事故发展有明显影响,模型中PRHRS余热排出能力较强,使冷却剂温度更快地降低到较低水平,导致CMT更早投入以及随后反应堆各参数响应的不同.%For the analysis of loss of normal feedwater accident for AP1000, a thermal-hydraulic model was built based on a two-fluid best estimate code, RELAP5. A control system model was built based on Matlab/Simulink code. As the main data for modeling was from AP1000 Design Control Document (AP1000 DCD), which was inadequate and not accurate enough, the accident analysis was qualitative. The calculation results, overall consistent with the same accident in DCD, show that the RELAP5 code has the ability to calculate the natural circulation. PRHRS and CMT are able to remove the core residual heat, decay heat timely and efficiently during the accident to guarantee the safety of the core. In the calculation model, the capability of PRHRS is stronger, which makes the coolant temperature quickly reduced to a lower level, leading to an earlier actuation of CMT, followed by different responses of the plant.

  6. Identification of ku70 as a Novel Transcriptional Coactivator of AP-2α%一类新的AP-2α转录共激活物Ku 70蛋白的鉴定

    Institute of Scientific and Technical Information of China (English)

    黄前川; 曹军皓; 季蒙

    2009-01-01

    目的 鉴定新的AP-2α转录共激活物,为乳腺癌的防治寻找新的靶点.方法 以AP-2α为诱饵采用酵母双杂交技术寻找新的AP-2α结合蛋白,免疫共沉淀证实AP-2α与其结合蛋白的相互作用,免疫荧光技术检测AP-2α与其结合蛋白的亚细胞共定位.荧光素酶报导基因检测AP-2α结合蛋白对AP-2α转录活性的影响.结果 酵母双杂交技术鉴定出Ku 70蛋白为新的AP-2α结合蛋白.免疫共沉淀证实了AP-2α和Ku 70蛋白在细胞内的相互作用.亚细胞共定位研究显示AP-2α和Ku 70蛋白都定位于细胞核内.在功能上发现Ku70可以增强AP-2α的转录活性.结论 本研究首次揭示Ku70可以结合AP-2α并促进其转录活性,证明Ku70是一类新的AB-2α的转录共激活物,提示Ku70有望成为治疗AP-2α高表达乳腺癌的新靶点.

  7. A Brief Introduction to Chinese Biological Biological

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    Chinese Biological Abstracts sponsored by the Library, the Shanghai Institutes for Biological Sciences, the Biological Documentation and Information Network, all of the Chinese Academy of Sciences, commenced publication in 1987 and was initiated to provide access to the Chinese information in the field of biology.

  8. Simulating Biological and Non-Biological Motion

    Science.gov (United States)

    Bruzzo, Angela; Gesierich, Benno; Wohlschlager, Andreas

    2008-01-01

    It is widely accepted that the brain processes biological and non-biological movements in distinct neural circuits. Biological motion, in contrast to non-biological motion, refers to active movements of living beings. Aim of our experiment was to investigate the mechanisms underlying mental simulation of these two movement types. Subjects had to…

  9. Prediction of three-dimensional femoral offset from AP pelvis radiographs in primary hip osteoarthritis

    Energy Technology Data Exchange (ETDEWEB)

    Merle, C., E-mail: christian.merle@med.uni-heidelberg.de [Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences, University of Oxford (United Kingdom); Department of Orthopaedic and Trauma Surgery, University Hospital Heidelberg (Germany); Waldstein, W., E-mail: wwaldstein@gmail.com [Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences, University of Oxford (United Kingdom); Department of Orthopaedic and Trauma Surgery, University Hospital Heidelberg (Germany); Pegg, E.C., E-mail: elise.pegg@ndorms.ox.ac.uk [Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences, University of Oxford (United Kingdom); Streit, M.R., E-mail: marcus.streit@med.uni-heidelberg.de [Department of Orthopaedic and Trauma Surgery, University Hospital Heidelberg (Germany); Gotterbarm, T., E-mail: tobias.gotterbarm@med.uni-heidelberg.de [Department of Orthopaedic and Trauma Surgery, University Hospital Heidelberg (Germany); Aldinger, P.R., E-mail: peter.aldinger@diak-stuttgart.de [Department of Orthopaedic Surgery, Paulinenhilfe, Diakonieklinikum, Stuttgart (Germany); Murray, D.W., E-mail: david.murray@ndorms.ox.ac.uk [Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences, University of Oxford (United Kingdom); Gill, H.S., E-mail: r.gill@bath.ac.uk [Department of Mechanical Engineering, University of Bath (United Kingdom)

    2013-08-15

    Background: In pre-operative planning for total hip arthroplasty (THA), femoral offset (FO) is frequently underestimated on AP pelvis radiographs as a result of inaccurate patient positioning, imprecise magnification, and radiographic beam divergence. The aim of the present study was to evaluate the accuracy and reliability of predicting three-dimensional (3-D) FO from standardised AP pelvis radiographs. Methods: In a retrospective cohort study, pre-operative AP pelvis radiographs, AP hip radiographs and CT scans of a consecutive series of 345 patients (345 hips, 146 males, 199 females, mean age 60 (range: 40–79) years, mean body-mass-index 27 (range: 19–57) kg/m{sup 2}) with primary end-stage hip OA were reviewed. Patients were positioned according to a standardised protocol and all images were calibrated. Using validated custom programmes, FO was measured on corresponding radiographs and CT scans. Measurement reliability was evaluated using intra-class-correlation-coefficients. To predict 3-D FO from AP pelvis measurements and to assess the accuracy compared to CT, the entire cohort was randomly split into subgroups A and B. Gender specific regression equations were derived from group A (245 patients) and the accuracy of prediction was evaluated in group B (100 patients) using Bland–Altman plots. Results: In the entire cohort, mean FO was 39.2 mm (95%CI: 38.5–40.0 mm) on AP pelvis radiographs, 44.1 mm (95%CI: 43.4–44.9 mm) on AP hip radiographs and 44.6 mm (95%CI: 44.0–45.2 mm) on CT scans. In group B, we observed no significant difference between gender specific predicted FO (males: 48.0 mm, 95%CI: 47.1–48.8 mm; females: 42.0 mm, 95%CI: 41.1–42.8 mm) and FO as measured on CT (males: 47.7 mm, 95%CI: 46.1–49.4 mm, p = 0.689; females: 41.6 mm, 95%CI: 40.3–43.0 mm, p = 0.607). Conclusions: The present study suggests that FO can be accurately and reliably predicted from AP pelvis radiographs in patients with primary end-stage hip osteoarthritis

  10. Dyslipidemia and its relationship with antiphospholipid antibodies in APS patients in North Kerala

    Science.gov (United States)

    Sadanand, Shajit; Paul, Binoy J; Thachil, Emil J; Meletath, Rejadheesh

    2016-01-01

    Objective Antiphospholipid antibody syndrome (APS) is one of the most common acquired thrombophilic disorders resulting in arterial and venous thromboses. APS is a major cause for cerebrovascular accidents or stokes, myocardial infarction, venous thromboembolism and recurrent abortions/pregnancy losses especially in young patients. APS patients have an increased risk of atherosclerotic cardiovascular events. There are only two studies on lipid abnormalities in APS patients. None of them have studied the relationship between individual laboratory tests for APS and lipid profile abnormalities. Here we describe the significance of the relationship between various APS tests and lipid profile abnormalities in a subset of APS patients who presented with arterial thrombosis in a tertiary care hospital. Material and Methods The study was conducted at Government Medical College, which is a tertiary care referral hospital. All patients who presented to the medicine department with APS during a two-year period were studied. A patient was considered to be positive for anticardiolipin (aCL) antibody or anti-β2 glycoprotein (anti-β2G) if the titer was more than 15 IU/mL, and a high titer was considered to be more than 40 IU/mL for Immunoglobulin (lg) IgG and IgM isotypes. The fasting lipid profile was measured in all patients, and lipid profile abnormalities were defined with cutoffs of low-density lipoprotein (LDL) levels of >150 mg/dL, triglyceride (TG) levels of >150 mg/dL, and high-density lipoprotein (HDL) levels of 150 mg/dL in 51.9% of the patients, followed by low HDL levels (150 mg/dL) in 40.2% of the patients. There was a statistically significant relationship between anti-β2G IgG levels and HDL and LDL levels, but not TG levels. Only LDL levels had a statistically significant relationship with aCL IgM levels. None of the lipid abnormalities had any statistically significant relationship with aCL IgG levels. Conclusion This study highlights the importance of testing

  11. APS kinase from Arabidopsis thaliana: genomic organization, expression, and kinetic analysis of the recombinant enzyme.

    Science.gov (United States)

    Lee, S; Leustek, T

    1998-06-09

    The gene encoding 5'-adenylylsulfate (APS) kinase (EC 2.7.1.25) (APK) was cloned from Arabidopsis thaliana. There is a single APK locus in A. thaliana. The coding sequence of the gene is composed of 7 exons, interrupted by 6 introns. A transcriptional initiation site was detected 120 bp 5' of the initiation codon. APK mRNA is slightly more abundant in leaves than in roots of A. thaliana and its level does not change in response to sulfur starvation. The APK protein, synthesized in vitro, is able to enter isolated intact chloroplasts. Recombinant APS kinase shows maximal activity at 10 microM APS with 5 mM ATP, but it is inhibited at APS concentrations above 10 microM. The inhibition is alleviated at higher ATP concentrations. Reciprocal plot analysis showed that the theoretical Vmax is approximately 1.2 mumol min-1 mg-1 at 25 degrees C, pH 8.0; the K(m) values are 3.6 microM APS and 1.8 mM ATP.

  12. Identification of an AP2-family protein that is critical for malaria liver stage development.

    Directory of Open Access Journals (Sweden)

    Shiroh Iwanaga

    Full Text Available Liver-stage malaria parasites are a promising target for drugs and vaccines against malaria infection. However, little is currently known about gene regulation in this stage. In this study, we used the rodent malaria parasite Plasmodium berghei and showed that an AP2-family transcription factor, designated AP2-L, plays a critical role in the liver-stage development of the parasite. AP2-L-depleted parasites proliferated normally in blood and in mosquitoes. However, the ability of these parasites to infect the liver was approximately 10,000 times lower than that of wild-type parasites. In vitro assays showed that the sporozoites of these parasites invaded hepatocytes normally but that their development stopped in the middle of the liver schizont stage. Expression profiling using transgenic P. berghei showed that fluorescent protein-tagged AP2-L increased rapidly during the liver schizont stage but suddenly disappeared with the formation of the mature liver schizont. DNA microarray analysis showed that the expression of several genes, including those of parasitophorous vacuole membrane proteins, was significantly decreased in the early liver stage of AP2-L-depleted parasites. Investigation of the targets of this transcription factor should greatly promote the exploration of liver-stage antigens and the elucidation of the mechanisms of hepatocyte infection by malaria parasites.

  13. A Novel AP for Improving the Performance of Wireless LANs Supporting VoIP

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    Thavisak Manodham

    2006-08-01

    Full Text Available Recently, wireless LANs based on the IEEE 802.11 standard have become popular and widely used, and Voice-over-IP (VoIP is one of the most promising technologies to be used in mobile devices over wireless networks. Up-to-date, new technologies have improved, providing a high data rate to support various kinds of realtime applications. However, besides the need for higher data rates, seamless handoff is another important issue to be addressed in order to continue supporting real-time services across wireless LANs. In this paper we introduce a novel Access Point (AP with two transceivers to improve network efficiency in terms of supporting seamless handoff and balancing the traffic load in a wireless network. In our proposed scheme, the novel AP will use the second transceiver to scan and find neighboring STAs in the transmission range and later send the result to its associate AP, which in turn compares and analyzes whether or not the STA should perform a handoff. The initial results from our simulations show the novel AP module is more effective than the conventional one in terms of providing a handoff process with low latency and support traffic load sharing with neighbor APs.

  14. Origination, expansion, evolutionary trajectory, and expression bias of AP2/ERF superfamily in Brassica napus

    Directory of Open Access Journals (Sweden)

    Xiaoming Song

    2016-08-01

    Full Text Available The AP2/ERF superfamily, one of the most important transcription factor families, plays crucial roles in response to biotic and abiotic stresses. So far, a comprehensive evolutionary inference of its origination and expansion has not been available. Here, we identified 515 AP2/ERF genes in B. napus, a neo-tetraploid forming ~7500 years ago, and found that 82.14% of them were duplicated in the tetraploidization. A prominent subgenome bias was revealed in gene expression, tissue-specific, and gene conversion. Moreover, a large-scale analysis across plants and alga suggested that this superfamily could have been originated from AP2 family, expanding to form other families (ERF, and RAV. This process was accompanied by duplicating and/or alternative deleting AP2 domain, intragenic domain sequence conversion, and/or by acquiring other domains, resulting in copy number variations, alternatively contributing to functional innovation. We found that significant positive selection occurred at certain critical nodes during the evolution of land plants, possibly responding to changing environment. In conclusion, the present research revealed origination, functional innovation, and evolutionary trajectory of the AP2/ERF superfamily, contributing to understanding their roles in plant stress tolerance.

  15. Enhancing QoS Through Dynamic and Fare AP Selection in a Wireless LAN

    Directory of Open Access Journals (Sweden)

    Fakhar Uddin Ahmed

    2014-01-01

    Full Text Available The IEEE 802.11 WLAN is primarily used for web browsing which belongs to the category of non-real time application. But the demand of real time applications like VOIP and video conferencing has become very much common to such WLAN. With IEEE 802.11e Mac protocol it is possible to improve the QoS for both real and non-real time traffic by service differentiation. To ensure efficient utilization of the radio resources and enhanced QoS the load imbalance should be resolved among APs from different BSSs. In large scale WLAN inter AP communication mechanism can be employed along side the current admission controller under EDCA. Beside service differentiation inter AP differentiation based QoS management can lead to efficient utilization of radio resources by moving STAs from heavily laded to a less loaded AP and ensure better QoS for all types of traffics. In this paper we propose a dynamic and fair AP selection mechanism to improve the QoS in a WLAN. The simulations have been carried out with NSv2.34.

  16. AP-2{alpha} suppresses skeletal myoblast proliferation and represses fibroblast growth factor receptor 1 promoter activity

    Energy Technology Data Exchange (ETDEWEB)

    Mitchell, Darrion L. [Department of Cell Biology and Anatomy, Chicago Medical School, Rosalind Franklin University of Medicine and Science, 3333 Green Bay Road, North Chicago, IL 60064 (United States); DiMario, Joseph X., E-mail: joseph.dimario@rosalindfranklin.edu [Department of Cell Biology and Anatomy, Chicago Medical School, Rosalind Franklin University of Medicine and Science, 3333 Green Bay Road, North Chicago, IL 60064 (United States)

    2010-01-15

    Skeletal muscle development is partly characterized by myoblast proliferation and subsequent differentiation into postmitotic muscle fibers. Developmental regulation of expression of the fibroblast growth factor receptor 1 (FGFR1) gene is required for normal myoblast proliferation and muscle formation. As a result, FGFR1 promoter activity is controlled by multiple transcriptional regulatory proteins during both proliferation and differentiation of myogenic cells. The transcription factor AP-2{alpha} is present in nuclei of skeletal muscle cells and suppresses myoblast proliferation in vitro. Since FGFR1 gene expression is tightly linked to myoblast proliferation versus differentiation, the FGFR1 promoter was examined for candidate AP-2{alpha} binding sites. Mutagenesis studies indicated that a candidate binding site located at - 1035 bp functioned as a repressor cis-regulatory element. Furthermore, mutation of this site alleviated AP-2{alpha}-mediated repression of FGFR1 promoter activity. Chromatin immunoprecipitation studies demonstrated that AP-2{alpha} interacted with the FGFR1 promoter in both proliferating myoblasts and differentiated myotubes. In total, these results indicate that AP-2{alpha} is a transcriptional repressor of FGFR1 gene expression during skeletal myogenesis.

  17. Effects of environmental estrogenic chemicals on AP1 mediated transcription with estrogen receptors alpha and beta.

    Science.gov (United States)

    Fujimoto, Nariaki; Honda, Hiroaki; Kitamura, Shigeyuki

    2004-01-01

    There has been much discussion concerning endocrine disrupting chemicals suspected of exerting adverse effects in both wildlife and humans. Since the majority of these compounds are estrogenic, a large number of in vitro tests for estrogenic characteristics have been developed for screening purpose. One reliable and widely used method is the reporter gene assay employing estrogen receptors (ERs) and a reporter gene with a cis-acting estrogen responsive element (ERE). Other elements such as AP1 also mediate estrogenic signals and the manner of response could be quite different from that of ERE. Since this has yet to be explored, the ER mediated AP1 activity in response to a series of environmental estrogens was investigated in comparison with ERE findings. All the compounds exhibited estrogenic properties with ERE-luc and their AP1 responses were quite similar. These was one exception, however, p,p'-DDT (1,1,1,-trichloro-2,2-bis(p-chlorophenyl)ethane) did not exert any AP1-luc activity, while it appeared to be estrogenic at 10(-7) to 10(-5)M with the ERE action. None of the compounds demonstrated ER beta:AP1 activity. These data suggest that significant differences can occur in responses through the two estrogen pathways depending on environmental chemicals.

  18. Origination, Expansion, Evolutionary Trajectory, and Expression Bias of AP2/ERF Superfamily in Brassica napus

    Science.gov (United States)

    Song, Xiaoming; Wang, Jinpeng; Ma, Xiao; Li, Yuxian; Lei, Tianyu; Wang, Li; Ge, Weina; Guo, Di; Wang, Zhenyi; Li, Chunjin; Zhao, Jianjun; Wang, Xiyin

    2016-01-01

    The AP2/ERF superfamily, one of the most important transcription factor families, plays crucial roles in response to biotic and abiotic stresses. So far, a comprehensive evolutionary inference of its origination and expansion has not been available. Here, we identified 515 AP2/ERF genes in B. napus, a neo-tetraploid forming ~7500 years ago, and found that 82.14% of them were duplicated in the tetraploidization. A prominent subgenome bias was revealed in gene expression, tissue-specific, and gene conversion. Moreover, a large-scale analysis across plants and alga suggested that this superfamily could have been originated from AP2 family, expanding to form other families (ERF, and RAV). This process was accompanied by duplicating and/or alternative deleting AP2 domain, intragenic domain sequence conversion, and/or by acquiring other domains, resulting in copy number variations, alternatively contributing to functional innovation. We found that significant positive selection occurred at certain critical nodes during the evolution of land plants, possibly responding to changing environment. In conclusion, the present research revealed origination, functional innovation, and evolutionary trajectory of the AP2/ERF superfamily, contributing to understanding their roles in plant stress tolerance. PMID:27570529

  19. Optimizing TCP Performance in Multi-AP Residential Broadband Connections via Minislot Access

    Directory of Open Access Journals (Sweden)

    Domenico Giustiniano

    2013-01-01

    Full Text Available The high bandwidth demand of Internet applications has recently driven the need of increasing the residential download speed. A practical solution to the problem is to aggregate the bandwidth of 802.11 access points (APs backhauls in range. Since 802.11 devices are usually single radio, the communication to APs on different radio channels requires a time-division multiple access (TDMA policy at the client station. With an in-depth experimental analysis and a customized 802.11 driver, in this paper, we show that the usage of multi-AP TDMA policy may cause degradation of the TCP throughput and an underutilization of the AP backhauls. We then introduce a simple analytical model that accurately predicts the TCP round-trip time (RTT with a multi-AP TDMA policy and propose a resource allocation algorithm to reduce the observed TCP RTT with a very low computational cost. Our proposed scheme runs locally at the client station and improves the aggregate throughput up to 1.5 times compared to state-of-the-art allocations. We finally show that the throughput achieved by our algorithm is very close to the theoretical upper bound in key simulation scenarios.

  20. Characterization of the Trichomonas vaginalis surface-associated AP65 and binding domain interacting with trichomonads and host cells.

    Science.gov (United States)

    Garcia, Ana F; Alderete, Jf

    2007-12-25

    AP65 is a prominent adhesin of Trichomonas vaginalis that mediates binding of parasites to host vaginal epithelial cells (VECs). AP65 with no secretion signal sequence, membrane targeting peptide, and anchoring motif was recently found to be secreted. We first wanted to demonstrate surface association of AP65 to the parasite followed by the identification of the binding epitope interacting with both organisms and VECs. AP65 was found to bind to trichomonads, but not to trypsin-treated parasites, in an auto-ligand assay, suggesting the existence of a surface protein associating with AP65. Since rabbit antiserum IgG antibodies reactive with epitopes localized to the N-terminal region of AP65 inhibit the attachment of live parasites to VECs, we hypothesized that the binding domain was localized to this region. We subcloned five overlapping fragments of AP65 called c1 through c5, and expression of recombinant clones was confirmed with antibodies to AP65. Each purified recombinant protein was then tested for binding activity using an established ligand assay, and fragment c1 with the first twenty-five amino acids in the N-terminal domain was required for binding to VECs and, surprisingly, also to parasites. Importantly, c1 competed with the binding of AP65 to both cells types. T. vaginalis AP65 is a secreted, surface-associated protein and a model is proposed to explain how this secreted protein functions as an adhesin.

  1. Characterization of the Trichomonas vaginalis surface-associated AP65 and binding domain interacting with trichomonads and host cells

    Directory of Open Access Journals (Sweden)

    Alderete JF

    2007-12-01

    Full Text Available Abstract Background AP65 is a prominent adhesin of Trichomonas vaginalis that mediates binding of parasites to host vaginal epithelial cells (VECs. AP65 with no secretion signal sequence, membrane targeting peptide, and anchoring motif was recently found to be secreted. Results We first wanted to demonstrate surface association of AP65 to the parasite followed by the identification of the binding epitope interacting with both organisms and VECs. AP65 was found to bind to trichomonads, but not to trypsin-treated parasites, in an auto-ligand assay, suggesting the existence of a surface protein associating with AP65. Since rabbit antiserum IgG antibodies reactive with epitopes localized to the N-terminal region of AP65 inhibit the attachment of live parasites to VECs, we hypothesized that the binding domain was localized to this region. We subcloned five overlapping fragments of AP65 called c1 through c5, and expression of recombinant clones was confirmed with antibodies to AP65. Each purified recombinant protein was then tested for binding activity using an established ligand assay, and fragment c1 with the first twenty-five amino acids in the N-terminal domain was required for binding to VECs and, surprisingly, also to parasites. Importantly, c1 competed with the binding of AP65 to both cells types. Conclusion T. vaginalis AP65 is a secreted, surface-associated protein and a model is proposed to explain how this secreted protein functions as an adhesin.

  2. Ectopic expression of transcription factor AP-2δ in developing retina: effect on PSA-NCAM and axon routing.

    Science.gov (United States)

    Li, Xiaodong; Monckton, Elizabeth A; Godbout, Roseline

    2014-04-01

    Retinal ganglion cells transmit the visual signal from the retina to the brain. We have previously shown that the activator protein 2 (AP-2)δ (TFAP2D) transcription factor is expressed in one third of ganglion cells in developing retina suggesting a specialized role for these AP-2δ-expressing cells. Here, we address the role of AP-2δ in retina by in ovo electroporation of RCAS/AP-2δ retroviral constructs into the eyes of chick embryos at day 2 of gestation. Ectopic expression of AP-2δ does not affect lineage differentiation in the developing retina. However, immunostaining of retinal tissue with markers associated with axonal growth such as growth-associated protein 43 and polysialic acid-neural cell adhesion molecule (PSA-NCAM) demonstrates axonal misrouting and abnormal axonal bundling. Treatment of AP-2δ-misexpressing retinal cell cultures with endoneuraminidase, an enzyme that removes PSA from NCAM, decreases AP-2δ-induced axonal bundling. Our data suggest a role for AP-2δ in polysialylation of NCAM, with ectopic expression of AP-2δ resulting in premature bundling of emerging axons and misrouting of axons. We propose that expression of AP-2δ in a subset of ganglion cells contributes to the fine-tuning of axonal growth in the developing retina.

  3. Evolution and protein interactions of AP2 proteins in Brassicaceae:Evidence linking development and environmental responses

    Institute of Scientific and Technical Information of China (English)

    Liping Zeng; Yue Yin; Chenjiang You; Qianli Pan; Duo Xu; Taijie Jin; Bailong Zhang; and Hong Ma

    2016-01-01

    Plants have evolved a large number of transcrip-tion factors (TF), which are enriched among duplicate genes, highlighting their roles in complex regulatory networks. The APETALA2/EREBP-like genes constitute a large plant TF family and participate in development and stress responses. To probe the conservation and divergence of AP2/EREBP genes, we analyzed the duplication patterns of this family in Brassicaceae and identified interacting proteins of represen-tative Arabidopsis AP2/EREBP proteins. We found that many AP2/EREBP duplicates generated early in Brassicaceae history were quickly lost, but many others were retained in all tested Brassicaceae species, suggesting early functional divergence followed by persistent conservation. In addition, the sequences of the AP2 domain and exon numbers were highly conserved in rosids. Furthermore, we used 16 A. thaliana AP2/EREBP proteins as baits in yeast screens and identified 1,970 potential AP2/EREBP-interacting proteins, with a small subset of interactions verified in planta. Many AP2 genes also exhibit reduced expression in an anther- defective mutant, providing a possible link to developmental regulation. The putative AP2-interacting proteins participate in many functions in development and stress responses, including photomorphogenesis, flower development, path-ogenesis, drought and cold responses, abscisic acid and auxin signaling. Our results present the AP2/EREBP evolution patterns in Brassicaceae, and support a proposed interaction network of AP2/EREBP proteins and their putative interacting proteins for further study.

  4. AP2γ regulates neural and epidermal development downstream of the BMP pathway at early stages of ectodermal patterning

    Institute of Scientific and Technical Information of China (English)

    Yunbo Qiao; Yue Zhu; Nengyin Sheng; Jun Chen; Ran Tao; Qingqing Zhu; Ting Zhang; Cheng Qian; Naihe Jing

    2012-01-01

    Bone morphogenetic protein (BMP) inhibits neural specification and induces epidermal differentiation during ectodermal patterning.However,the mechanism of this process is not well understood.Here we show that AP2γ,a transcription factor activator protein (AP)-2 family member,is upregulated by BMP4 during neural differentiation of pluripotent stem cells.Knockdown of AP2γ facilitates mouse embryonic stem cell (ESC) neural fate determination and impairs epidermal differentiation,whereas AP2γ overexpression inhibits neural conversion and promotes epidermal commitment.In the early chick embryo,AP2γ is expressed in the entire epiblast before HH stage 3 and gradually shifts to the putative epidermal ectoderm during HH stage 4.In the future neural plate AP2γ inhibits excessive neural expansion and it also promotes epidermal development in the surface ectoderm.Moreover,AP2γ knockdown in ESCs and chick embryos partially rescued the neural inhibition and epidermal induction effects of BMP4.Mechanistic studies showed that BMP4 directly regulates AP2γ expression through Smad1 binding to the AP2γ promoter.Taken together,we propose that during the early stages of ectodermal patterning in the chick embryo,AP2γ acts downstream of the BMP pathway to restrict precocious neural expansion in the prospective neural plate and initiates epidermal differentiation in the future epidermal ectoderm.

  5. AP1000控制棒驱动线落棒试验研究%Study on Drop Performance Test of AP1000 Control Rod Drive Line

    Institute of Scientific and Technical Information of China (English)

    顾汉洋; 张朝柱; 陈宇清; 周肖佳; 刘刚

    2015-01-01

    采用1组全尺寸AP1000控制棒驱动线进行落棒试验,研究不同纵向流和横向流以及错位和变形对落棒时间的影响,并用高速摄像图像处理技术获得落棒行程速度曲线.试验结果表明,纵向流量是影响驱动线落棒时间的主要因素;在本试验范围内横向流以及部件的错位变形对AP1000控制棒驱动线落棒时间的影响都较为有限.

  6. The novel mitochondria-targeted hydrogen sulfide (H2S) donors AP123 and AP39 protect against hyperglycemic injury in microvascular endothelial cells in vitro.

    Science.gov (United States)

    Gerő, Domokos; Torregrossa, Roberta; Perry, Alexis; Waters, Alicia; Le-Trionnaire, Sophie; Whatmore, Jacqueline L; Wood, Mark; Whiteman, Matthew

    2016-11-01

    The development of diabetic vascular complications is initiated, at least in part, by mitochondrial reactive oxygen species (ROS) production in endothelial cells. Hyperglycemia induces superoxide production in the mitochondria and initiates changes in the mitochondrial membrane potential that leads to mitochondrial dysfunction. Hydrogen sulfide (H2S) supplementation has been shown to reduce the mitochondrial oxidant production and shows efficacy against diabetic vascular damage in vivo. However, the half-life of H2S is very short and it is not specific for the mitochondria. We have therefore evaluated two novel mitochondria-targeted anethole dithiolethione and hydroxythiobenzamide H2S donors (AP39 and AP123 respectively) at preventing hyperglycemia-induced oxidative stress and metabolic changes in microvascular endothelial cells in vitro. Hyperglycemia (HG) induced significant increase in the activity of the citric acid cycle and led to elevated mitochondrial membrane potential. Mitochondrial oxidant production was increased and the mitochondrial electron transport decreased in hyperglycemic cells. AP39 and AP123 (30-300nM) decreased HG-induced hyperpolarisation of the mitochondrial membrane and inhibited the mitochondrial oxidant production. Both H2S donors (30-300nM) increased the electron transport at respiratory complex III and improved the cellular metabolism. Targeting H2S to mitochondria retained the cytoprotective effect of H2S against glucose-induced damage in endothelial cells suggesting that the molecular target of H2S action is within the mitochondria. Mitochondrial targeting of H2S also induced >1000-fold increase in the potency of H2S against hyperglycemia-induced injury. The high potency and long-lasting effect elicited by these H2S donors strongly suggests that these compounds could be useful against diabetic vascular complications. Copyright © 2016 The Authors. Published by Elsevier Ltd.. All rights reserved.

  7. An MHC-I cytoplasmic domain/HIV-1 Nef fusion protein binds directly to the mu subunit of the AP-1 endosomal coat complex.

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    Rajendra Kumar Singh

    Full Text Available BACKGROUND: The down-regulation of the major histocompatibility complex class I (MHC-I from the surface of infected cells by the Nef proteins of primate immunodeficiency viruses likely contributes to pathogenesis by providing evasion of cell-mediated immunity. HIV-1 Nef-induced down-regulation involves endosomal trafficking and a cooperative interaction between the cytoplasmic domain (CD of MHC-I, Nef, and the clathrin adaptor protein complex-1 (AP-1. The CD of MHC-I contains a key tyrosine within the sequence YSQA that is required for down-regulation by Nef, but this sequence does not conform to the canonical AP-binding tyrosine-based motif Yxxphi, which mediates binding to the medium (micro subunits of AP complexes. We previously proposed that Nef allows the MHC-I CD to bind the mu subunit of AP-1 (micro1 as if it contained a Yxxphimotif. METHODS AND FINDINGS: Here, we show that a direct interaction between the MHC-I CD/Nef and micro1 plays a primary role in the down-regulation of MHC-I: GST pulldown assays using recombinant proteins indicated that most of the MHC-I CD and Nef residues that are required for the down-regulation in human cells contribute to direct interactions with a truncated version of micro1. Specifically, the tyrosine residue of the YSQA sequence in the MHC-I CD as well as Nef residues E62-65 and P78 each contributed to the interaction between MHC-I CD/Nef and micro1 in vitro, whereas Nef M20 had little to no role. Conversely, residues F172/D174 and V392/L395 of the binding pocket on micro1 for Yxxphi motifs were required for a robust interaction. CONCLUSIONS: These data indicate that the MHC-I cytoplasmic domain, Nef, and the C-terminal two thirds of the mu subunit of AP-1 are sufficient to constitute a biologically relevant interaction. The data also reveal an unexpected role for a hydrophobic pocket in micro1 for interaction with MHC-I CD/Nef.

  8. Estêvão e a Ação Popular (AP: a história da AP nas memórias de Duarte Pereira

    Directory of Open Access Journals (Sweden)

    Reginaldo Benedito Dias

    2016-10-01

    Full Text Available O objetivo deste artigo é analisar como a trajetória da Ação Popular (AP, organização de esquerda das décadas de 1960 e 1970, é narrada e interpretada por Duarte Pacheco Pereira, um dos mais importantes dirigentes de sua história. Caracterizada por sua origem heterodoxa, influenciada pelo humanismo cristão, pelo existencialismo e pelo marxismo, a AP estabeleceu, na conjuntura posterior a 1964, um processo de ruptura com seu passado e de adesão à tradição do marxismo-leninismo, que foi permeado por tensões e cisões políticas. Investigando a interface entre as suas memórias e a história da AP, procura-se aferir sua contribuição à interpretação da singular práxis dessa organização política. A análise tem o suporte de entrevistas concedidas por Duarte Pacheco Pereira, de uma palestra que ele proferiu e do fundo documental que leva seu nome, disponível no Arquivo Edgard Leuenroth.

  9. Adaptor Protein Complexes AP-1 and AP-3 Are Required by the HHV-7 Immunoevasin U21 for Rerouting of Class I MHC Molecules to the Lysosomal Compartment

    Science.gov (United States)

    Kimpler, Lisa A.; Glosson, Nicole L.; Downs, Deanna; Gonyo, Patrick; May, Nathan A.; Hudson, Amy W.

    2014-01-01

    The human herpesvirus-7 (HHV-7) U21 gene product binds to class I major histocompatibility complex (MHC) molecules and reroutes them to a lysosomal compartment. Trafficking of integral membrane proteins to lysosomes is mediated through cytoplasmic sorting signals that recruit heterotetrameric clathrin adaptor protein (AP) complexes, which in turn mediate protein sorting in post-Golgi vesicular transport. Since U21 can mediate rerouting of class I molecules to lysosomes even when lacking its cytoplasmic tail, we hypothesize the existence of a cellular protein that contains the lysosomal sorting information required to escort class I molecules to the lysosomal compartment. If such a protein exists, we expect that it might recruit clathrin adaptor protein complexes as a means of lysosomal sorting. Here we describe experiments demonstrating that the μ adaptins from AP-1 and AP-3 are involved in U21-mediated trafficking of class I molecules to lysosomes. These experiments support the idea that a cellular protein(s) is necessary for U21-mediated lysosomal sorting of class I molecules. We also examine the impact of transient versus chronic knockdown of these adaptor protein complexes, and show that the few remaining μ subunits in the cells are eventually able to reroute class I molecules to lysosomes. PMID:24901711

  10. A band-tunable, multichannel amplifier for neural recording with AP/LFP separation and dual-threshold adaptive AP detector.

    Science.gov (United States)

    Wu, Jo-Yu; Tang, Kea-Tiong

    2011-01-01

    This article presents a low-power low-noise neural recording system comprising a set of 4-channel amplifiers and a dual-threshold adaptive action potential detector. The front-end amplifier is optimized for power efficiency, noise, and silicon area. A balanced tunable pseudo-resistor is used to acquire local field potential (LFP) and action potential (AP) separately. The post-layout simulation results show that the system achieved input referred noise 4.7 μVrms and noise efficiency factor (NEF) 2.79 with mid-band gain of 51.9 dB and power consumption of 5.22 μW. The bandwidth is highly tunable in the range of 2.38 Hz-300 Hz for high-pass corner and 248 Hz-12.9 kHz for low-pass corner, which can acquire AP and LPF without out-band noise. The proposed dual-threshold adaptive AP detector can capture action potential precisely from background activity, thus data reduction can be realized by only processing these significant waveforms. The results show that the proposed low-power, low-noise biomedical system is suitable for implantable device applications.

  11. Investigative cases and student outcomes in an upper-division cell and molecular biology laboratory course at a minority-serving institution.

    Science.gov (United States)

    Knight, Jonathan D; Fulop, Rebecca M; Márquez-Magaña, Leticia; Tanner, Kimberly D

    2008-01-01

    Active-learning strategies are increasingly being integrated into college-level science courses to make material more accessible to all students and to improve learning outcomes. One active-learning pedagogy, case-based learning (CBL), was developed as a way to both enhance engagement in the material and to accommodate diverse learning styles. Yet, adoption of CBL approaches in undergraduate biology courses has been piecemeal, in part because of the perceived investment of time required. Furthermore, few CBL lesson plans have been developed specifically for upper-division laboratory courses. Here, we describe four cases that we developed and implemented for a senior cell and molecular biology laboratory course at San Francisco State University, a minority-serving institution. To evaluate the effectiveness of these modules, we used both written and verbal assessments to gauge learning outcomes and attitudinal responses of students over two semesters. Students responded positively to the new approach and seemed to meet the learning goals for the course. Most said they would take a course using CBL again. These case modules are readily adaptable to a variety of classroom settings.

  12. High school and college biology: A multi-level model of the effects of high school biology courses on student academic performance in introductory college biology courses

    Science.gov (United States)

    Loehr, John Francis

    The issue of student preparation for college study in science has been an ongoing concern for both college-bound students and educators of various levels. This study uses a national sample of college students enrolled in introductory biology courses to address the relationship between high school biology preparation and subsequent introductory college biology performance. Multi-Level Modeling was used to investigate the relationship between students' high school science and mathematics experiences and college biology performance. This analysis controls for student demographic and educational background factors along with factors associated with the college or university attended. The results indicated that high school course-taking and science instructional experiences have the largest impact on student achievement in the first introductory college biology course. In particular, enrollment in courses, such as high school Calculus and Advanced Placement (AP) Biology, along with biology course content that focuses on developing a deep understanding of the topics is found to be positively associated with student achievement in introductory college biology. On the other hand, experiencing high numbers of laboratory activities, demonstrations, and independent projects along with higher levels of laboratory freedom are associated with negative achievement. These findings are relevant to high school biology teachers, college students, their parents, and educators looking beyond the goal of high school graduation.

  13. Topical Conference on Oportunities in Biology for Physicists II

    Energy Technology Data Exchange (ETDEWEB)

    Franz, Judy R.

    2004-02-01

    In 2002, the American Physical Society (APS) organized and held the first topical conference in Boston, MA, as a way of informing physicists, particularly those just entering the field, of opportunities emerging at the interface of physics and biology. Because of the tremendous success of the first conference, it was decided to organize a second conference, similar in nature and focus, but with different presentation topic areas. Again the intended audience would be graduate students and postdocs considering applying methods of physics to biological research, and those who advise others on such opportunities.

  14. Evaluation of Impulse Unloading Efficiency Pertaining to APS Power Block Turbine for Higher Dynamic Steadiness

    Directory of Open Access Journals (Sweden)

    Yu. Filipchik

    2012-01-01

    Full Text Available The paper considers the influence of impulse unloading of APS power block turbine on its dynamic steadiness. The performed calculations while simulating four various impulse turbine characteristics have shown that the highest effect is obtained while characteristics of APS power block turbine are close to the characteristics of a steam turbine K-300/240. It has been established that an application of standard time interval for emergency automation startup and existing delay in the operation of electro-hydraulic transformer do not allow to influence on acceleration area and the positive effect due to load shedding is reached only due to an increase of braking site. While decreasing time period for control signal supply from 0,2 sec to 0,1 sec it is possible to increase steadiness by 10 %  for impulse characteristics of APS power blocks and by 39,9–42,6 % – for characteristics of the turbine K-300/240.

  15. K-Nearest Neighbor Intervals Based AP Clustering Algorithm for Large Incomplete Data

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    Cheng Lu

    2015-01-01

    Full Text Available The Affinity Propagation (AP algorithm is an effective algorithm for clustering analysis, but it can not be directly applicable to the case of incomplete data. In view of the prevalence of missing data and the uncertainty of missing attributes, we put forward a modified AP clustering algorithm based on K-nearest neighbor intervals (KNNI for incomplete data. Based on an Improved Partial Data Strategy, the proposed algorithm estimates the KNNI representation of missing attributes by using the attribute distribution information of the available data. The similarity function can be changed by dealing with the interval data. Then the improved AP algorithm can be applicable to the case of incomplete data. Experiments on several UCI datasets show that the proposed algorithm achieves impressive clustering results.

  16. ANALISIS KONDISI TERAS REAKTOR DAYA MAJU AP1000 PADA KECELAKAAN SMALL BREAK LOCA

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    Andi Sofrany Ekariansyah

    2015-06-01

    Full Text Available ABSTRAK ANALISIS KONDISI TERAS REAKTOR DAYA MAJU AP1000 PADA KECELAKAAN SMALL BREAK LOCA. Kecelakaan yang diakibatkan oleh kehilangan pendingin (loss of coolant accident / LOCA dari sistem reaktor merupakan kejadian dasar desain yang tetap diantisipasi dalam desain reaktor daya yang mengadopsi teknologi Generasi II hingga IV. LOCA ukuran kecil (small break LOCA memiliki dampak yang lebih signifikan terhadap keselamatan dibandingkan LOCA ukuran besar (large break LOCA seperti terlihat pada kejadian Three-Mile Island (TMI. Fokus makalah adalah pada analisis small break LOCA pada reaktor daya maju Generasi III+ yaitu AP1000 dengan mensimulasikan tiga kejadian pemicu yaitu membukanya katup Automatic Depressurization System (ADS secara tak disengaja, putusnya salah satu pipa Direct Vessel Injection (DVI secara double-ended, dan putusnya pipa lengan dingin dengan diameter bocoran 10 inci. Metode yang digunakan adalah simulasi kejadian pada model AP1000 yang dikembangkan secara mandiri menggunakan program perhitungan RELAP5/SCDAP/Mod3.4. Dampak yang ingin dilihat adalah kondisi teras selama terjadinya small break LOCA yang terdiri dari pembentukan mixture level dan transien temperatur kelongsong bahan bakar. Hasil simulasi menunjukkan bahwa mixture level untuk semua kejadian small break LOCA berada di atas tinggi teras aktif yang menunjukkan tidak terjadinya core uncovery. Adanya mixture level berpengaruh pada transien temperatur kelongsong yang menurun dan menunjukkan pendinginan bahan bakar yang efektif. Hasil di atas juga identik dengan hasil perhitungan program lain yaitu NOTRUMP. Keefektifan pendinginan teras juga disebabkan oleh berfungsinya injeksi pendingin melalui fitur keselamatan pasif yang menjadi ciri reaktor daya AP1000. Secara keseluruhan, hasil analisis menunjukkan model AP1000 yang telah dikembangkan dengan RELAP5 dapat digunakan untuk keperluan analisis kecelakaan dasar desain pada reaktor daya maju AP1000. Kata kunci: analisis

  17. Magnetic fields of Ap stars from the full Stokes spectropolarimetric observations

    CERN Document Server

    Rusomarov, N; Piskunov, N

    2013-01-01

    Current knowledge about stellar magnetic fields relies almost entirely on circular polarization observations. Few objects have been observed in all four Stokes parameters. The magnetic Ap star HD 24712 (DO Eri, HR 1217) was recently observed in the Stokes IQUV parameters with the HARPSpol instrument at the 3.6-m ESO telescope as part of our project at investigating Ap stars in all four Stokes parameters. The resulting spectra have dense phase coverage, resolving power > 10^5, and S/N ratio of 300-600. These are the highest quality full Stokes observations obtained for any star other than the Sun. We present preliminary results from magnetic Doppler imaging of HD 24712. This analysis is the first step towards obtaining detailed 3-D maps of magnetic fields and abundance structures for HD 24712 and other Ap stars that we currently observe with HARPSpol.

  18. Monitoring physiology trainee needs to focus professional society responses: the APS Trainee Needs Surveys.

    Science.gov (United States)

    Matyas, Marsha L; Lowy, Melinda E; Sweazea, Karen L; Alvarez, Diego F

    2011-06-01

    In 2004 and 2007, the American Physiological Society (APS) Trainee Advisory Committee (TAC) conducted surveys of graduate students, postdoctoral fellows, and new investigators in physiology to identify topics and issues important to those trainees. Two major trends emerged from the data. First, trainees in 2007 expressed somewhat greater interest in professional development information than did those in 2004. Second, needs expressed by trainees in both years were closely related to their specific career development stage. Survey findings guided the TAC and other APS committees and groups to focus their efforts toward the issues that were of the greatest interest to trainees. It also led to improved communication with trainees and increased involvement of trainees in APS governance.

  19. Genomic and transcriptomic analysis of the AP2/ERF superfamily in Vitis vinifera

    Science.gov (United States)

    2010-01-01

    Background The AP2/ERF protein family contains transcription factors that play a crucial role in plant growth and development and in response to biotic and abiotic stress conditions in plants. Grapevine (Vitis vinifera) is the only woody crop whose genome has been fully sequenced. So far, no detailed expression profile of AP2/ERF-like genes is available for grapevine. Results An exhaustive search for AP2/ERF genes was carried out on the Vitis vinifera genome and their expression profile was analyzed by Real-Time quantitative PCR (qRT-PCR) in different vegetative and reproductive tissues and under two different ripening stages. One hundred and forty nine sequences, containing at least one ERF domain, were identified. Specific clusters within the AP2 and ERF families showed conserved expression patterns reminiscent of other species and grapevine specific trends related to berry ripening. Moreover, putative targets of group IX ERFs were identified by co-expression and protein similarity comparisons. Conclusions The grapevine genome contains an amount of AP2/ERF genes comparable to that of other dicot species analyzed so far. We observed an increase in the size of specific groups within the ERF family, probably due to recent duplication events. Expression analyses in different aerial tissues display common features previously described in other plant systems and introduce possible new roles for members of some ERF groups during fruit ripening. The presented analysis of AP2/ERF genes in grapevine provides the bases for studying the molecular regulation of berry development and the ripening process. PMID:21171999

  20. AP1000与传统核电厂紧急停堆功能对比%On the Emergency Shut-down Function Comparison of AP1000 and Traditional Nuclear Power Plants

    Institute of Scientific and Technical Information of China (English)

    李方栋

    2015-01-01

    对比AP1000与秦山一期、二期核电厂紧急停堆信号,探讨AP1000在紧急停堆信号设置上的优点及其可优化之处。%This paper compares the reactor trip signals of AP1000 plant with that of Qinshan Nuclear Power Plant Phase I/II. By doing this, we can find the advantage and possible shortcoming of the AP1000 plant reactor protection system.

  1. Cloning and Phylogeny Analysis of PpAP2 Floral Homologous Genes in Peach%毛桃开花相关基因PpAP2的克隆及其进化分析

    Institute of Scientific and Technical Information of China (English)

    冯涛; 彭立新; 阎国荣

    2013-01-01

    In order to investigate the control mechanism of peach flowering, the apl gene of peach was cloned. The peach (Prunus persica) cultivar 'Dongfeng' was used as material. In silico cloning PCR and RACE were employed to cloning complete cDNA sequence. The function was analyzed by bioinformatics methods. The gene had 1767 bp nucleotides and an ORF frame with 467 amino acid residue. The deduced protein had a molecular weight 52.0 kD, an equipotential point 8.73 and two copies of AP2 regions. PpAP2 was located in scaffold_6 in peach genome, and a SSR marker, namely AP2M anchored in it. Amino acid sequences alignment revealed that high similarity presented between PpAP2 and AP2 from other plant species, in which the highest homology found between PpAP2 and Malus x domestics AHAP2. The coincidence between AP2 genes revolution and species revolution was concluded.%为了探索毛桃开花的控制机制,开展了ap2基因的克隆研究.以毛桃品种‘冬丰’花芽为材料,应用电子克隆和PCR、RACE技术克隆ap2的cDNA全长序列,采用生物信息学方法分析基因的功能.PpAP2长度为1767bp,开放性阅读框编码467个氨基酸残基,推断其相对分子量为52.0 kD,等电点为8.73,含有2个AP2结构域;位于毛桃基因组的scaffold_6上,而名为AP2M的SSR标记位于PpAP2内部.氨基酸同源性分析表明,PpAP2与已报道的其他植物的AP2具有较高的相似性,其中与苹果AP2同源性最高.该类基因的进化与物种进化同步.

  2. Transcription factor AP-2delta regulates the expression of polysialyltransferase ST8SIA2 in chick retina.

    Science.gov (United States)

    Li, Xiaodong; Persad, Amit R L; Monckton, Elizabeth A; Godbout, Roseline

    2014-03-03

    The AP-2δ transcription factor is restricted to a subset of retinal ganglion cells. Overexpression of AP-2δ in chick retina results in induction of polysialylated neural cell adhesion molecule (PSA-NCAM) accompanied by misrouting and bundling of ganglion cell axons. Two polysialyltransferases, ST8SIA2 and ST8SIA4, are responsible for polysialylation of NCAM. Here, we investigate the mechanism driving the increase in PSA-NCAM observed upon AP-2δ overexpression. We show that ST8SIA2 is induced by AP-2δ overexpression in chick retina. We use chromatin immunoprecipitation and gel shift assays to demonstrate direct interaction between AP-2δ and the ST8SIA2 promoter. We propose that up-regulation of ST8SIA2 upon AP-2δ overexpression in retina increases ectopic polysialylation of NCAM which in turn causes premature bundling of axons and alters axonal response to guidance cues.

  3. AP1000非能动换热器数值模拟%Numerical simulation of AP1000 passive residual heat removal heat exchanger

    Institute of Scientific and Technical Information of China (English)

    薛若军; 王明远; 李朝君; 祝贺

    2012-01-01

    To accurately know heat transfer and flow processes of AP1000 passive residual heat removal heat exchanger( PRHR HX) , and provide a reference for the safe operation of our new AP1000 advanced pressurized water reactor nuclear power plant, FLUENT software is used. By comparative analysis of the distribution of temperature-field and flow-field in different locations at the same time, and the change of temperature-field and flow-field in the same location at different times, we can know the heat transfer and~flow characteristics which are changed with time more intuitive, and analyze the effect of straight tubes and elbow tubes on the natural circulation. The results show that the temperature difference, vortex, flow around make the interaction of temperature field and flow field. Due to the influence of elbow tubes structure, the fluid natural convection in the IRWST is more complex.%为准确认识AP1000非能动余热排出热交换器的具体换热和流动过程,针对AP1000非能动换热器,应用FLUENT软件对其非稳态传热特性和流动进行研究,比较分析同一时刻不同位置温度场和流场的分布,以及不同时刻同一位置温度场和流场的变化,分析换热器传热和流动特性随时间的变化,同时对比分析直管形管束和弯形管束对换热器自然对流的影响.分析结果表明:温差、漩涡和回流使温度场和流场相互影响,弯形管束使流体自然对流更加复杂.研究成果有助于分析换热器的自然循环能力,为我国新建的AP1000先进压水堆核电机组的安全运行提供参考.

  4. Cell biology perspectives in phage biology.

    Science.gov (United States)

    Ansaldi, Mireille

    2012-01-01

    Cellular biology has long been restricted to large cellular organisms. However, as the resolution of microscopic methods increased, it became possible to study smaller cells, in particular bacterial cells. Bacteriophage biology is one aspect of bacterial cell biology that has recently gained insight from cell biology. Despite their small size, bacteriophages could be successfully labeled and their cycle studied in the host cells. This review aims to put together, although non-extensively, several cell biology studies that recently pushed the elucidation of key mechanisms in phage biology, such as the lysis-lysogeny decision in temperate phages or genome replication and transcription, one step further.

  5. Parte segunda de las excelencias y primacias del glorioso Apóstol Santiago ...

    OpenAIRE

    2013-01-01

    T. I.: Parte primera de las excelencias del glorioso Apóstol Santiago ... / Antonio Calderón. -- En Madrid : Por Gregorio Rodríguez, 1658 - T. II.: Parte segunda de las excelencias y primacias del glorioso Apóstol Santiago ... / Gerónimo Pardo. -- En Madrid : Por Gregorio Rodríguez, 1657 1 v. (18, 189, 2, 443, 36 p.); 31 cm 2 t. en 1 v. Tít. na portadilla : Parte primera y segunda de las excelencias del Apostol Santiago Front. calc. e alegórico na port.: "Pº de Villafranca sculpto...

  6. Prevalencia de enfermedad diverticular del apéndice cecal en pacientes apendicectomizados por apendicitis aguda

    OpenAIRE

    Muñoz C,César; Mansilla E,Juan; Roa S,Juan Carlos; Heider C,Claudia

    2011-01-01

    Introducción: La enfermedad diverticular del apéndice cecal es un hallazgo infrecuente en apendicectomizados por sospecha clínica de apendicitis aguda. Nuestro objetivo es evaluar la prevalencia de enfermedad diverticular del apéndice cecal en pacientes apendicectomizados por sospecha clínica de apendicitis aguda. Material y Método: Diseño de estudio: Estudio de corte transversal. Criterios de inclusión: Pacientes apendicectomizados por sospecha clínica de apendicitis aguda. Período de estudi...

  7. Prevalencia de enfermedad diverticular del apéndice cecal en pacientes apendicectomizados por apendicitis aguda

    OpenAIRE

    MUÑOZ C,CÉSAR; MANSILLA E,JUAN; Roa S,Juan Carlos; Heider C,Claudia

    2011-01-01

    Introducción: La enfermedad diverticular del apéndice cecal es un hallazgo infrecuente en apendicectomizados por sospecha clínica de apendicitis aguda. Nuestro objetivo es evaluar la prevalencia de enfermedad diverticular del apéndice cecal en pacientes apendicectomizados por sospecha clínica de apendicitis aguda. Material y Método: Diseño de estudio: Estudio de corte transversal. Criterios de inclusión: Pacientes apendicectomizados por sospecha clínica de apendicitis aguda. Período de estudi...

  8. Induction of aP2 gene expression by nonmetabolized long-chain fatty acids.

    OpenAIRE

    Grimaldi, P A; Knobel, S.M.; Whitesell, R R; Abumrad, N A

    1992-01-01

    Long-chain fatty acids (FA) have been shown to regulate expression of the gene for the adipocyte FA-binding protein aP2. We examined whether this effect was exerted by FA themselves or by a FA metabolite. The alpha-bromo derivative of palmitate, an inhibitor of FA oxidation, was synthesized in the radioactive form, and its metabolism was investigated and correlated with its ability to induce aP2 in Ob1771 preadipocytes. alpha-Bromopalmitate was not utilized by preadipocytes. It was not cleare...

  9. My max score AP statistics maximize your score in less time

    CERN Document Server

    Ross, Phd, Amanda

    2013-01-01

    The only study guide to offer expert, customized study plans for every student's needs You've had a year to study...but also a year to forget. As the AP test approaches, other guides reexamine the entire year of material. But only one guide identifies your strengths and weaknesses, then points you directly to the review you need most My Max Score, a new concept developed by AP teachers and exam graders, offers separate review materials for long-term prep and last-minute cram sessions-no matter when you start studying, This is just what you need-plus str

  10. Analytical Model of Large Data Transactions in CoAP Networks

    OpenAIRE

    Alessandro Ludovici; Piergiuseppe Di Marco; Anna Calveras; Johansson, Karl H.

    2014-01-01

    We propose a novel analytical model to study fragmentation methods in wireless sensor networks adopting the Constrained Application Protocol (CoAP) and the IEEE 802.15.4 standard for medium access control (MAC). The blockwise transfer technique proposed in CoAP and the 6LoWPAN fragmentation are included in the analysis. The two techniques are compared in terms of reliability and delay, depending on the traffic, the number of nodes and the parameters of the IEEE 802.15.4 MAC. The results are v...

  11. A Survey of Floor Vibration Noise at All Sectors in the APS Experiment Hall

    Energy Technology Data Exchange (ETDEWEB)

    Kearney, Steven [Argonne National Lab. (ANL), Argonne, IL (United States); Shu, Deming [Argonne National Lab. (ANL), Argonne, IL (United States)

    2016-01-01

    A vibration survey of the APS experiment hall floor was conducted. It was found that beamlines 10-20 have particularly low levels of vibration when compared to the rest of the facility. The vibration spectrum for each beamline floor can be found in the appendix. Throughout the majority of the 5-100 Hz vibration spectrum beamlines at the APS fall below the most stringent NEST vibration criteria. Lastly, it was concluded that the magnitude of vibrations at a particular beamline is largely dependent upon the magnitude of vibrations present at the nearby mezzanine support column.

  12. AP1000核电稳压器大锻件特点及制造技术

    Institute of Scientific and Technical Information of China (English)

    张智峰; 李向; 陶志勇; 赵富

    2012-01-01

    为满足AP1000核电严格的技术要求,稳压器采用高性能大型锻件制造。介绍了AP1000核电稳压器大锻件的技术特点和制造技术。%In order to meet the AP1000 nuclear power strict technical requirements;manufacturing pressurizer need high performance heavy forgings. This paper introduces technical characteristics and manufacturing technology of heavy forgings for the AP1000 nuclear power pressurizer.

  13. Inactivation of AP1 proteins by a nuclear serine protease precedes the onset of radiation-induced fibrosing alveolitis.

    Science.gov (United States)

    Haase, Michael G; Klawitter, Anke; Bierhaus, Angelika; Yokoyama, Kazunari K; Kasper, Michael; Geyer, Peter; Baumann, Michael; Baretton, Gustavo B

    2008-05-01

    Radiation-induced lung damage comprises inflammation (alveolitis) as well as disturbed regulation of cell differentiation and proliferation (fibrosis). The transcriptional regulation of this process is poorly understood. One key transcription factor involved in the regulation of proliferation and differentiation is AP1 (activator protein 1). The present study examined changes in the DNA-binding activity of AP1 after irradiation and defined the underlying molecular mechanisms in an animal model. The right lungs of Fischer rats received a single radiation dose of 20 Gy. Lung tissue was tested for AP1 DNA-binding activity, AP1 mRNA, and levels of AP1 proteins as well as for c-Jun specific proteolytic activity. After an initial increase, the AP1 DNA-binding activity was completely lost starting at 5.5 weeks after irradiation, which is 2.5 weeks before the onset of fibrosing alveolitis. This was not caused by reduction of mRNA levels or size. Instead, a selective nuclear cleavage of c-Jun by a serine protease caused the loss of AP1 activity. Considering the central role of AP1 in cell proliferation and differentiation and the strict timely correlation to the onset of the disease, the complete loss of AP1 function is likely to play a critical role in radiation-induced fibrosing alveolitis.

  14. CD2AP is associated with end-stage renal disease in patients with type 1 diabetes

    DEFF Research Database (Denmark)

    Hyvönen, Mervi E; Ihalmo, Pekka; Sandholm, Niina;

    2013-01-01

    CD2-associated protein (CD2AP) is essential for podocyte function. CD2AP mutations have been found in patients with focal segmental glomerulosclerosis, a disease histologically resembling diabetic nephropathy and often progressing to end-stage renal disease (ESRD). We hypothesised that variations...... in the CD2AP gene may contribute to susceptibility to glomerular injury in diabetes and investigated if single-nucleotide polymorphisms (SNPs) in CD2AP are associated with diabetic nephropathy in patients with type 1 diabetes. The discovery cohort consisted of 2,251 Finnish patients with type 1 diabetes...

  15. Fatores associados à bacteriúria após sondagem vesical na cirurgia ginecológica

    OpenAIRE

    2009-01-01

    OBJETIVOS: Determinar a frequência e os principais fatores associados à bacteriúria após a sondagem vesical em mulheres submetidas à cirurgia ginecológica eletiva. MÉTODOS: Realizou-se um estudo do tipo coorte em mulheres submetidas à cirurgia ginecológica após sondagem vesical no Instituto de Medicina Integral Prof. Fernando Figueira, no período de janeiro a maio de 2007. As uroculturas foram coletadas até 24 horas após a retirada da sonda e 7/10 dias após a sondagem vesical. A análise estat...

  16. Strengthening cancer biology research, prevention, and control while reducing cancer disparities: student perceptions of a collaborative master's degree program in cancer biology, preventions, and control.

    Science.gov (United States)

    Jillson, I A; Cousin, C E; Blancato, J K

    2013-09-01

    This article provides the findings of a survey of previous and current students in the UDC/GU-LCCC master's degree program. This master's degree program, Cancer Biology, Prevention, and Control is administered and taught jointly by faculty of a Minority Serving Institution, the University of the District of Columbia, and the Lombardi Comprehensive Cancer Center to incorporate the strengths of a community-based school with a research intensive medical center. The program was initiated in 2008 through agreements with both University administrations and funding from the National Cancer Institute. The master's degree program is 36 credits with a focus on coursework in biostatistics, epidemiology, tumor biology, cancer prevention, medical ethics, and cancer outreach program design. For two semesters during the second year, students work full-time with a faculty person on a laboratory or outreach project that is a requirement for graduation. Students are supported and encouraged to transition to a doctoral degree after they obtain the master's and many of them are currently in doctorate programs. Since the inception of the program, 45 students have initiated the course of study, 28 have completed the program, and 13 are currently enrolled in the program. The survey was designed to track the students in their current activities, as well as determine which courses, program enhancements, and research experiences were the least and most useful, and to discern students' perceptions of knowledge acquired on various aspects of Cancer Biology Prevention, and Control Master's Program. Thirty of the 35 individuals to whom email requests were sent responded to the survey, for a response rate of 85.7%. The results of this study will inform the strengthening of the Cancer Biology program by the Education Advisory Committee. They can also be used in the development of comparable collaborative master's degree programs designed to address the significant disparities in prevalence of

  17. Cooperative stabilization of Mycobacterium tuberculosis rrnAP3 promoter open complexes by RbpA and CarD

    Science.gov (United States)

    Rammohan, Jayan; Ruiz Manzano, Ana; Garner, Ashley L.; Prusa, Jerome; Stallings, Christina L.; Galburt, Eric A.

    2016-01-01

    The essential mycobacterial transcriptional regulators RbpA and CarD act to modulate transcription by associating to the initiation complex and increasing the flux of transcript production. Each of these factors interacts directly with the promoter DNA template and with RNA polymerase (RNAP) holoenzyme. We recently reported on the energetics of CarD-mediated open complex stabilization on the Mycobacterium tuberculosis rrnAP3 ribosomal promoter using a stopped-flow fluorescence assay. Here, we apply this approach to RbpA and show that RbpA stabilizes RNAP-promoter open complexes (RPo) via a distinct mechanism from that of CarD. Furthermore, concentration-dependent stopped-flow experiments with both factors reveal positive linkage (cooperativity) between RbpA and CarD with regard to their ability to stabilize RPo. The observation of positive linkage between RbpA and CarD demonstrates that the two factors can act on the same transcription initiation complex simultaneously. Lastly, with both factors present, the kinetics of open complex formation is significantly faster than in the presence of either factor alone and approaches that of E. coli RNAP on the same promoter. This work provides a quantitative framework for the molecular mechanisms of these two essential transcription factors and the critical roles they play in the biology and pathology of mycobacteria. PMID:27342278

  18. Johnston Atoll Site 2A-P 6/30/2000 17-18M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 2A-P (16 45.815N, 169 30.706W), between 17 and 18 meters along a permanent transect.

  19. Johnston Atoll Site 1A-P 6/29/2000 6-7M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 1A-P (16 47.170N, 169 27.908W), between 6 and 7 meters along a permanent transect.

  20. Johnston Atoll Site 1A-P 6/29/2000 35-36M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 1A-P (16 47.170N, 169 27.908W), between 35 and 36 meters along a permanent transect.

  1. Johnston Atoll Site 3A-P 7/1/2000 6-7M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 3A-P (16 45.260N, 169 31.039W), between 6 and 7 meters along a permanent transect.

  2. Johnston Atoll Site 1A-P 1/23/2006 28-29M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 1A-P (16 46.909N, 169 27.757W), between 28 and 29 meters along a permanent transect.

  3. Johnston Atoll Site 1A-P 6/29/2000 31-32M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 1A-P (16 47.170N, 169 27.908W), between 31 and 32 meters along a permanent transect.

  4. Johnston Atoll Site 2A-P 6/30/2000 8-9M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 2A-P (16 45.815N, 169 30.706W), between 8 and 9 meters along a permanent transect.

  5. Johnston Atoll Site 1A-P 1/23/2006 2-3M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 1A-P (16 46.909N, 169 27.757W), between 2 and 3 meters along a permanent transect.

  6. Johnston Atoll Site 2A-P 6/30/2000 5-6M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 2A-P (16 45.815N, 169 30.706W), between 5 and 6 meters along a permanent transect.

  7. Johnston Atoll Site 2A-P 6/30/2000 13-14M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 2A-P (16 45.815N, 169 30.706W), between 13 and 14 meters along a permanent transect.

  8. Johnston Atoll Site 1A-P 1/23/2006 3-4M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 1A-P (16 46.909N, 169 27.757W), between 3 and 4 meters along a permanent transect.

  9. Johnston Atoll Site 1A-P 6/29/2000 29-30M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 1A-P (16 47.170N, 169 27.908W), between 29 and 30 meters along a permanent transect.

  10. Johnston Atoll Site 2A-P 6/30/2000 31-32M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 2A-P (16 45.815N, 169 30.706W), between 31 and 32 meters along a permanent transect.

  11. Johnston Atoll Site 1A-P 1/23/2006 8-9M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 1A-P (16 46.909N, 169 27.757W), between 8 and 9 meters along a permanent transect.

  12. Johnston Atoll Site 1A-P 6/29/2000 38-39M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 1A-P (16 47.170N, 169 27.908W), between 38 and 39 meters along a permanent transect.

  13. Johnston Atoll Site 2A-P 6/30/2000 15-16M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 2A-P (16 45.815N, 169 30.706W), between 15 and 16 meters along a permanent transect.

  14. EVALUATION OF THE TEMPORARY TENT COVER TRUSS SYSTEM AP PRIMARY VENT SYSTEM

    Energy Technology Data Exchange (ETDEWEB)

    HAQ MA

    2009-12-31

    The purpose of this calculation is to evaluate a temporary ten cover truss system. This system will be used to provide weather protection to the workers during replacement of the filter for the Primary Ventilation System in AP Tank Farm. The truss system has been fabricated utilizing tubes and couplers, which are normally used for scaffoldings.

  15. Discovery AP2/ERF family genes in silico in Medicago truncatula

    African Journals Online (AJOL)

    aghomotsegin

    Medicago truncatula is a legume model plant due to its small genome and it has been used to study the molecular ... To investigate the roles of AP2/EREBP in M. truncatula, ..... Zhuang J, Chen JM, Yao QH, Xiong F, Sun CC, Zhou XR, Zhang J,.

  16. Johnston Atoll Site 1A-P 1/23/2006 46-47M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 1A-P (16 46.909N, 169 27.757W), between 46 and 47 meters along a permanent transect.

  17. Energy expenditure and bone formation share a common sensitivity to AP-1 transcription in the hypothalamus

    DEFF Research Database (Denmark)

    Rowe, Glenn C; Vialou, Vincent; Sato, Kazusa

    2012-01-01

    to the regulation of both energy balance and bone homeostasis, little is known of the underlying mechanisms, including the possible involvement of transcriptional factors within the hypothalamus. Transgenic mice overexpressing ¿FosB, a splice variant of the AP1 transcription factor FosB with mixed agonist...

  18. Johnston Atoll Site 3A-P 7/1/2000 28-29M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 3A-P (16 45.260N, 169 31.039W), between 28 and 29 meters along a permanent transect.

  19. AP-3 and Rabip4' coordinately regulate spatial distribution of lysosomes.

    Directory of Open Access Journals (Sweden)

    Viorica Ivan

    Full Text Available The RUN and FYVE domain proteins rabip4 and rabip4' are encoded by RUFY1 and differ in a 108 amino acid N-terminal extension in rabip4'. Their identical C terminus binds rab5 and rab4, but the function of rabip4s is incompletely understood. We here found that silencing RUFY1 gene products promoted outgrowth of plasma membrane protrusions, and polarized distribution and clustering of lysosomes at their tips. An interactor screen for proteins that function together with rabip4' yielded the adaptor protein complex AP-3, of which the hinge region in the β3 subunit bound directly to the FYVE domain of rabip4'. Rabip4' colocalized with AP-3 on a tubular subdomain of early endosomes and the extent of colocalization was increased by a dominant negative rab4 mutant. Knock-down of AP-3 had an ever more dramatic effect and caused accumulation of lysosomes in protrusions at the plasma membrane. The most peripheral lysosomes were localized beyond microtubules, within the cortical actin network. Our results uncover a novel function for AP-3 and rabip4' in regulating lysosome positioning through an interorganellar pathway.

  20. [An investigation on fast thermolysis of ammonium perchlorate (AP) by FTIR spectroscopy].

    Science.gov (United States)

    Zhang, La-ying; Liu, Zi-ru; Wang, Xiao-hong; Heng, Shu-yun; Pan, Qing; Shao, Ying-hui; Zhang, Gao; Zhao, Feng-qi

    2010-08-01

    The fast thermolysis processes of ammonium perchlorate (AP) and its chemical reaction mechanism under certain simulated conditions of combustion were systematically studied by means of T-Jump/FTIR technique. AP was flash-pyrolyzed under different pressure nitrogen atmosphere, with the heating rate of 1000 K x s(-1) and the set temperatures of 874 and 1 274 K. Thereafter, the real time species and concentration of its gaseous products were obtained and identified by using rapid scanning Fourier transform infrared (FTIR) in-situ spectroscopy. It was indicated that the main gaseous products of AP thermolysis is composed of NO2, N2O, NO, HCl and NClO. Interestingly, the values of N2O/NO2, NO/NO2 and NO/NClO are increased while the test temperature or pressure is elevated. It is suggested that the transformation of condensed phase and heterogeneous gas/condensed phase was probably arises and the "following reactions" among the main gaseous products may also take place in the fast thermolysis process of AP.

  1. Johnston Atoll Site 1A-P 6/29/2000 8-9M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 1A-P (16 47.170N, 169 27.908W), between 8 and 9 meters along a permanent transect.

  2. Johnston Atoll Site 1A-P 1/23/2006 39-40M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 1A-P (16 46.909N, 169 27.757W), between 39 and 40 meters along a permanent transect.

  3. Johnston Atoll Site 2A-P 6/30/2000 12-13M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 2A-P (16 45.815N, 169 30.706W), between 12 and 13 meters along a permanent transect.

  4. Johnston Atoll Site 1A-P 1/23/2006 27-28M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 1A-P (16 46.909N, 169 27.757W), between 27 and 28 meters along a permanent transect.

  5. Johnston Atoll Site 1A-P 6/29/2000 0-1M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 1A-P (16 47.170N, 169 27.908W), between 0 and 1 meters along a permanent transect.

  6. Johnston Atoll Site 1A-P 1/23/2006 6-7M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 1A-P (16 46.909N, 169 27.757W), between 6 and 7 meters along a permanent transect.

  7. Johnston Atoll Site 1A-P 1/23/2006 5-6M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 1A-P (16 46.909N, 169 27.757W), between 5 and 6 meters along a permanent transect.

  8. Displacement damage effects on CMOS APS image sensors induced by neutron irradiation from a nuclear reactor

    Directory of Open Access Journals (Sweden)

    Zujun Wang

    2014-07-01

    Full Text Available The experiments of displacement damage effects on CMOS APS image sensors induced by neutron irradiation from a nuclear reactor are presented. The CMOS APS image sensors are manufactured in the standard 0.35 μm CMOS technology. The flux of neutron beams was about 1.33 × 108 n/cm2s. The three samples were exposed by 1 MeV neutron equivalent-fluence of 1 × 1011, 5 × 1011, and 1 × 1012 n/cm2, respectively. The mean dark signal (KD, dark signal spike, dark signal non-uniformity (DSNU, noise (VN, saturation output signal voltage (VS, and dynamic range (DR versus neutron fluence are investigated. The degradation mechanisms of CMOS APS image sensors are analyzed. The mean dark signal increase due to neutron displacement damage appears to be proportional to displacement damage dose. The dark images from CMOS APS image sensors irradiated by neutrons are presented to investigate the generation of dark signal spike.

  9. Comparing Recent Organizing Templates for Test Content between ACS Exams in General Chemistry and AP Chemistry

    Science.gov (United States)

    Holme, Thomas

    2014-01-01

    Two different versions of "big ideas" rooted content maps have recently been published for general chemistry. As embodied in the content outline from the College Board, one of these maps is designed to guide curriculum development and testing for advanced placement (AP) chemistry. The Anchoring Concepts Content Map for general chemistry…

  10. Comparing Recent Organizing Templates for Test Content between ACS Exams in General Chemistry and AP Chemistry

    Science.gov (United States)

    Holme, Thomas

    2014-01-01

    Two different versions of "big ideas" rooted content maps have recently been published for general chemistry. As embodied in the content outline from the College Board, one of these maps is designed to guide curriculum development and testing for advanced placement (AP) chemistry. The Anchoring Concepts Content Map for general chemistry…

  11. Characterization and free radical scavenging activity of rapeseed meal polysaccharides WPS-1 and APS-2.

    Science.gov (United States)

    Zhu, Jianfei; Wu, Moucheng

    2009-02-11

    Two major polysaccharide fractions, WPS-1 and APS-2, were isolated from water-soluble and alkali-soluble extracts of Huaza No. 4 rapeseed meal with a stepwise procedure of D3520 macroporous adsorption resin column chromatography, ethanol precipitation, and DE-52 cellulose column chromatography. Physicochemical properties of the polysaccharides were determined by chemical methods, high -performance liquid chromatography (HPLC), gel permeation chromatography (GPC), and Fourier transform infrared spectrometry (FT-IR). The chemiluminescence (CL) method was used to investigate the free radical scavenging activity of the polysaccharide fractions. The polysaccharides were primarily polymers of arabinose, galactose, and glucose, associated with protein portions consisting of 13 different amino acids. The average molecular masses of WPS-1 and APS-2 were 7.20 x 10(5) and 1.61 x 10(5) Da, respectively. Compared with APS-2, WPS-1 was more effective at scavenging superoxide radical (O(2)(*-)) and hydroxyl radical (HO(*)), but less effective at scavenging hydrogen peroxide (H(2)O(2)). In decreasing order, the free radical scavenging activity of WPS-1 and APS-2 toward reactive oxygen species (ROS) was H(2)O(2) > HO(*) > O(2)(*-).

  12. Supporting document for the historical tank content estimate for AP-tank farm

    Energy Technology Data Exchange (ETDEWEB)

    Brevick, C.H.; Stroup, J.L.; Funk, J.W., Fluor Daniel Hanford

    1997-03-06

    This Supporting Document provides historical in-depth characterization information on AP-Tank Farm, such as historical waste transfer and level data, tank physical information, temperature plots, liquid observation well plots, chemical analyte and radionuclide inventories for the Historical Tank Content Estimate Report for the Southeast Quadrant of the Hanford 200 Areas.

  13. Average Anisotropy Characteristics of High Energy Cosmic Ray Particles and Geomagnetic Disturbance Index Ap

    Indian Academy of Sciences (India)

    C. M. Tiwari; D. P. Tiwari; Ajay K. Pandey; Pankaj K. Shrivastava

    2005-12-01

    The average characteristics of the diurnal and semi-diurnal anisotropy of cosmic ray intensity at relativistic energies have been obtained by using data from the worldwide grid of neutron monitor for the period 1989 to 1996. The complex behaviour of the diurnal amplitudes and time of maxima (phase) and its association with the Ap index on a long-term and day-to-day basis have been studied. Even though the general characteristics, on a yearly average basis, have not changed significantly during this period, both the diurnal and semi-diurnal amplitudes and phases vary significantly, besides significant changes being observed for different interplanetary conditions on a short-term basis. It is found that the relationship between the Ap index and the diurnal vector is out of phase during the period 1991 to 1995. On a long-term basis, the correlation of diurnal variation with Ap index has been found to vary during the solar cycle. On a short-term basis, it has been observed that the high Ap days are usually associated with higher amplitudes with phase shifted to earlier hours.

  14. Johnston Atoll Site 1A-P 6/29/2000 24-25M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 1A-P (16 47.170N, 169 27.908W), between 24 and 25 meters along a permanent transect.

  15. Johnston Atoll Site 1A-P 1/23/2006 48-49M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 1A-P (16 46.909N, 169 27.757W), between 48 and 49 meters along a permanent transect.

  16. Accelerated aging of AP/HTPB propellants and the influence of various environmental aging conditions

    NARCIS (Netherlands)

    Keizers, H.L.J.

    1995-01-01

    Preliminary resuits on accelerated aging of lab-scale produced AP/HTPB propellant and propellants from dissectioned rocket motors are discussed, including aging logic, storage conditions, test techniques and resuits on mechanical, ballistic and safety testing. The mam aging effect observed was harde

  17. Johnston Atoll Site 1A-P 1/23/2006 33-34M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 1A-P (16 46.909N, 169 27.757W), between 33 and 34 meters along a permanent transect.

  18. KorAP: the new corpus analysis platform at IDS Mannheim

    DEFF Research Database (Denmark)

    Banski, Piotr; Bingel, Joachim; Diewald, Nils

    2013-01-01

    The KorAP project (“Korpusanalyseplattform der nächste Generation”, “Corpus-analysis platform of the next generation”), carried out at the Institut für Deutsche Sprache (IDS) in Mannheim, Germany, has as its goal the development of a modern, state-of-the-art corpus-analysis platform, capable...

  19. Johnston Atoll Site 1A-P 1/23/2006 4-5M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 1A-P (16 46.909N, 169 27.757W), between 4 and 5 meters along a permanent transect.

  20. Johnston Atoll Site 1A-P 1/23/2006 7-8M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 1A-P (16 46.909N, 169 27.757W), between 7 and 8 meters along a permanent transect.

  1. Johnston Atoll Site 2A-P 6/30/2000 27-28M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 2A-P (16 45.815N, 169 30.706W), between 27 and 28 meters along a permanent transect.

  2. Johnston Atoll Site 2A-P 6/30/2000 14-15M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 2A-P (16 45.815N, 169 30.706W), between 14 and 15 meters along a permanent transect.

  3. Johnston Atoll Site 1A-P 1/23/2006 29-30M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 1A-P (16 46.909N, 169 27.757W), between 29 and 30 meters along a permanent transect.

  4. The TeV emission of Ap Librae: a hadronic interpretation and prospects for CTA

    CERN Document Server

    Petropoulou, Maria; Giannios, Dimitrios

    2016-01-01

    Ap Librae is one out of a handful of low-frequency peaked blazars to be detected at TeV $\\gamma$-rays and the only one with an identified X-ray jet. Combined observations of Fermi-LAT at high energies (HE) and of H.E.S.S at very high energies (VHE) revealed a striking spectral property of Ap Librae; the presence of a broad high-energy component that extends more than nine orders of magnitude in energy and is, therefore, hard to be explained by the usual single-zone synchrotron self-Compton model. We show that the superposition of different emission components related to photohadronic interactions can explain the $\\gamma$-ray emission of Ap Librae without invoking external radiation fields. We present two indicative model fits to the spectral energy distribution of Ap Librae where the VHE emission is assumed to originate from a compact, sub-pc scale region of the jet. A robust prediction of our model is VHE flux variability on timescales similar to those observed at X-rays and HE $\\gamma$-rays, which can be fu...

  5. Another Strategy for Teaching Histology to A&P Students: Classification versus Memorization.

    Science.gov (United States)

    Bavis, Ryan W.; Seveyka, Jerred; Shigeoka, Cassie A.

    2000-01-01

    Defines dichotomous keys as common learning tools based on identification rather than memorization. Provides an example of a dichotomous key developed for introducing histology in human anatomy and physiology (A&P) courses and explains how students can use the dichotomous key. Discusses the goals of the exercises and the process of…

  6. Johnston Atoll Site 1A-P 1/23/2006 20-21M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 1A-P (16 46.909N, 169 27.757W), between 20 and 21 meters along a permanent transect.

  7. Frênulo lingual: modificações após frenectomia

    National Research Council Canada - National Science Library

    Marchesan, Irene Queiroz; Martinelli, Roberta Lopes de Castro; Gusmão, Reinaldo Jordão

    2012-01-01

    OBJETIVO: Descrever as mudanças ocorridas após a frenectomia com relação à mobilidade e funções da língua. MÉTODOS: Foram avaliados 53 sujeitos, os quais nunca haviam se submetido a fonoterapia ou a cirurgia...

  8. Pharmaceutical development of the novel metal-based anticancer agents NAMI-A and AP 5280

    NARCIS (Netherlands)

    Bouma, M. (Marjan)

    2002-01-01

    The pharmaceutical development of the two novel metal-based anticancer agents NAMI-A and AP 5280 is described in this thesis, starting with the development of analytical methods for the quality control of drug substance and final product, via the formulation process leading to a stable, intravenous

  9. Testing the presence of lithium on the surfaces of cool Ap stars

    Science.gov (United States)

    Nesvacil, N.; Hubrig, S.; Mathys, G.

    2004-12-01

    The possibility of a quite high Li abundance in the Ap stars was first raised by Wallerstein & Merchant (1965). Since then many studies investigated the problem of Li. The more recent observations in the lithium region indicate that in some Ap stars the λ 6708 feature is variable and this variability can be explained by the existence of Li rich spots on the stellar surface. Atomic data for the Ce II λ 6708.099 were released by the D.R.E.A.M. database in 2002. The line was used to identify the prominent suspected Li-feature in post AGB stars and might as well be responsible for the absorption feature in Ap stars. Recent studies have mentioned this possibility, but it has yet to be investigated in more detail. Other physical phenomena, such as the occurrence of a partial Paschen-Back effect in the presence of magnetic fields, as well as possible hyperfine structure splitting of some Rare Earth transitions, must be taken into account to provide correct line identifications in the wavelength region around the Li-doublet at λ 6708. We discuss a possible strategy to clarify the presence of Li in Ap stars.

  10. NFκB and AP-1 Drive Human Myometrial IL8 Expression

    Directory of Open Access Journals (Sweden)

    Shirin Khanjani

    2012-01-01

    Full Text Available The uterine expression of the chemokine IL8 increases dramatically with the onset of labour both at term and preterm. The IL8 promoter contains binding sites for the transcription factors nuclear factor-kappa B (NFκB, activator protein-1 (AP-1, and CCAAT/enhancer-binding protein (CEBP. In this study we investigated the roles of these transcription factors in IL1B regulation of the IL8 gene in human myometrium. Using chromatin immune precipitation (ChIP assay, we showed that each of NFκB, CEBP, and AP-1 binds to the IL8 promoter upon IL1B stimulation. To examine the relative importance of each site in IL8 gene expression, site-directed mutagenesis of each of these sites was performed. We found that the NFκB site was essential for basal and IL1B-stimulated gene expression. Mutation of the AP-1 site reduced both basal and IL1B-stimulated expression but to a lesser extent. Mutation of the CEBP site had no effect upon basal expression but eliminated the IL1B response. Small interfering RNA (siRNA silencing of NFκB abolished the IL8 response to IL1B significantly; siRNA against AP-1 reduced it to a lesser extent whilst knockdown of CEBP enhanced the response. Our data confirms a central and essential role for NFκB in regulation of IL8 in human myometrium.

  11. Professional Development Aligned with AP Chemistry Curriculum: Promoting Science Practices and Facilitating Enduring Conceptual Understanding

    Science.gov (United States)

    Herrington, Deborah G.; Yezierski, Ellen J.

    2014-01-01

    The recent revisions to the advanced placement (AP) chemistry curriculum promote deep conceptual understanding of chemistry content over more rote memorization of facts and algorithmic problem solving. For many teachers, this will mean moving away from traditional worksheets and verification lab activities that they have used to address the vast…

  12. A Test of Strategies for Enhanced Learning of AP Descriptive Chemistry

    Science.gov (United States)

    Kotcherlakota, Suhasini; Brooks, David W.

    2008-01-01

    The Advanced Placement (AP) Descriptive Chemistry Website allows users to practice chemistry problems. This study involved the redesign of the Website using worked examples to enhance learner performance. The population sample for the study includes users (students and teachers) interested in learning descriptive chemistry materials. The users…

  13. Johnston Atoll Site 1A-P 1/23/2006 43-44M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 1A-P (16 46.909N, 169 27.757W), between 43 and 44 meters along a permanent transect.

  14. Johnston Atoll Site 1A-P 1/23/2006 13-14M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 1A-P (16 46.909N, 169 27.757W), between 13 and 14 meters along a permanent transect.

  15. Johnston Atoll Site 1A-P 1/23/2006 40-41M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 1A-P (16 46.909N, 169 27.757W), between 40 and 41 meters along a permanent transect.

  16. Johnston Atoll Site 1A-P 6/29/2000 26-27M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 1A-P (16 47.170N, 169 27.908W), between 26 and 27 meters along a permanent transect.

  17. Johnston Atoll Site 2A-P 6/30/2000 33-34M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 2A-P (16 45.815N, 169 30.706W), between 33 and 34 meters along a permanent transect.

  18. Johnston Atoll Site 3A-P 7/1/2000 14-15M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 3A-P (16 45.260N, 169 31.039W), between 14 and 15 meters along a permanent transect.

  19. Another Strategy for Teaching Histology to A&P Students: Classification versus Memorization.

    Science.gov (United States)

    Bavis, Ryan W.; Seveyka, Jerred; Shigeoka, Cassie A.

    2000-01-01

    Defines dichotomous keys as common learning tools based on identification rather than memorization. Provides an example of a dichotomous key developed for introducing histology in human anatomy and physiology (A&P) courses and explains how students can use the dichotomous key. Discusses the goals of the exercises and the process of…

  20. Johnston Atoll Site 2A-P 6/30/2000 3-4M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 2A-P (16 45.815N, 169 30.706W), between 3 and 4 meters along a permanent transect.

  1. Johnston Atoll Site 1A-P 1/23/2006 10-11M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 1A-P (16 46.909N, 169 27.757W), between 10 and 11 meters along a permanent transect.

  2. Development and evaluation of human AP endonuclease inhibitors in melanoma and glioma cell lines

    DEFF Research Database (Denmark)

    Mohammed, M Z; Vyjayanti, V N; Laughton, C A

    2011-01-01

    Modulation of DNA base excision repair (BER) has the potential to enhance response to chemotherapy and improve outcomes in tumours such as melanoma and glioma. APE1, a critical protein in BER that processes potentially cytotoxic abasic sites (AP sites), is a promising new target in cancer. In the...

  3. Accelerated aging of AP/HTPB propellants and the influence of various environmental aging conditions

    NARCIS (Netherlands)

    Keizers, H.L.J.

    1995-01-01

    Preliminary resuits on accelerated aging of lab-scale produced AP/HTPB propellant and propellants from dissectioned rocket motors are discussed, including aging logic, storage conditions, test techniques and resuits on mechanical, ballistic and safety testing. The mam aging effect observed was

  4. Rotation and oblique pulsation in Kepler observations of the roAp star KIC 10483436

    DEFF Research Database (Denmark)

    Balona, L. A.; Cunha, M. S.; Gruberbauer, M.

    2011-01-01

    Photometry of KIC 10483436 was obtained continuously with 1-min exposures over a 27-d period from the Kepler satellite. The light curve shows rotational variations from surface spots with a period of 4.303 ± 0.002 d, an amplitude of about 6 mmag and eight pulsation frequencies typical of roAp stars...

  5. Synthesis and properties of ApA analogues with shortened phosphonate internucleotide linkage.

    Science.gov (United States)

    Králíková, Sárka; Buděšínský, Miloš; Barvík, Ivan; Masojídková, Milena; Točík, Zdeněk; Rosenberg, Ivan

    2011-01-01

    A complete series of the 2 '-5 ' and 3 '-5 ' regioisomeric types of r(ApA) and 2 '-d(ApA) analogues with the α-hydroxy-phosphonate C3 '-O-P-CH(OH)-C4 ″ internucleotide linkage, isopolar but non-isosteric with the phosphodiester one, were synthesized and their hybridization properties with polyU studied. Due to the chirality on the 5 '-carbon atom of the modified internucleotide linkage bearing phosphorus and hydroxy moieties, each regioisomeric type of ApA dimer is split into epimeric pairs. To examine the role of the 5 '-hydroxyl of the α-hydroxy-phosphonate moiety during hybridization, the appropriate r(ApA) analogues with 3 '(2 ')-O-P-CH(2)-C4 ″ linkage lacking the 5 '-hydroxyl were synthesized. Nuclear magnetic resonance (NMR) spectroscopy study on the conformation of the modified sugar-phosphate backbone, along with the hybridization measurements, revealed remarkable differences in the stability of complexes with polyU, depending on the 5 '-carbon atom configuration. Potential usefulness of the α-hydroxy-phosphonate linkage in modified oligoribonucleotides is discussed.

  6. Thermodynamics of Damaged DNA Binding and Catalysis by Human AP Endonuclease 1.

    Science.gov (United States)

    Miroshnikova, A D; Kuznetsova, A A; Kuznetsov, N A; Fedorova, O S

    2016-01-01

    Apurinic/apyrimidinic (AP) endonucleases play an important role in DNA repair and initiation of AP site elimination. One of the most topical problems in the field of DNA repair is to understand the mechanism of the enzymatic process involving the human enzyme APE1 that provides recognition of AP sites and efficient cleavage of the 5'-phosphodiester bond. In this study, a thermodynamic analysis of the interaction between APE1 and a DNA substrate containing a stable AP site analog lacking the C1' hydroxyl group (F site) was performed. Based on stopped-flow kinetic data at different temperatures, the steps of DNA binding, catalysis, and DNA product release were characterized. The changes in the standard Gibbs energy, enthalpy, and entropy of sequential specific steps of the repair process were determined. The thermodynamic analysis of the data suggests that the initial step of the DNA substrate binding includes formation of non-specific contacts between the enzyme binding surface and DNA, as well as insertion of the amino acid residues Arg177 and Met270 into the duplex, which results in the removal of "crystalline" water molecules from DNA grooves. The second binding step involves the F site flipping-out process and formation of specific contacts between the enzyme active site and the everted 5'-phosphate-2'-deoxyribose residue. It was shown that non-specific interactions between the binding surfaces of the enzyme and DNA provide the main contribution into the thermodynamic parameters of the DNA product release step.

  7. An Index (PC) Aimed at Monitoring the (P)olar (C)ap for Magnetic Activity

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — PC is an index for magnetic activity in the (P)olar (C)ap. It is based on data from a single nearpole station, and aimed to monitor the polar cap magnetic activity...

  8. Johnston Atoll Site 1A-P 6/29/2000 37-38M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 1A-P (16 47.170N, 169 27.908W), between 37 and 38 meters along a permanent transect.

  9. Johnston Atoll Site 1A-P 6/29/2000 2-3M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 1A-P (16 47.170N, 169 27.908W), between 2 and 3 meters along a permanent transect.

  10. Johnston Atoll Site 1A-P 1/23/2006 1-2M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Johnston Atoll, site 1A-P (16 46.909N, 169 27.757W), between 1 and 2 meters along a permanent transect.

  11. 75 FR 53681 - Office of Elementary and Secondary Education Overview Information; Advanced Placement (AP) Test...

    Science.gov (United States)

    2010-09-01

    ... Office of Elementary and Secondary Education Overview Information; Advanced Placement (AP) Test Fee... Elementary and Secondary Education Act of 1965, as amended (ESEA), requires that grant funds provided under.... Thelma Mel ndez de Santa Ana, Assistant Secretary for Elementary and Secondary Education. BILLING...

  12. Lack of evidence for mutations or deletions in the CDKN2A/p16 and CDKN2B/p15 genes of Brazilian neuroblastoma patients

    Directory of Open Access Journals (Sweden)

    Bassi C.L.

    2004-01-01

    Full Text Available Neuroblastoma, the most common extracranial tumor in childhood, has a wide spectrum of clinical and biological features. The loss of heterozygosity within the 9p21 region has been reported as a prognostic factor. Two tumor suppressor genes located in this region, the CDKN2B/p15 and CDKN2A/p16 (cyclin-dependent kinase inhibitors 2B and 2A, respectively genes, play a critical role in cell cycle progression and are considered to be targets for tumor inactivation. We analyzed CDKN2B/p15 and CDKN2A/p16 gene alterations in 11 patients, who ranged in age from 4 months to 13 years (male/female ratio was 1.2:1. The most frequent stage of the tumor was stage IV (50%, followed by stages II and III (20% and stage I (10%. The samples were submitted to the multiplex PCR technique for homozygous deletion analysis and to single-strand conformation polymorphism and nucleotide sequencing for mutation analysis. All exons of both genes were analyzed, but no deletion was detected. One sample exhibited shift mobility specific for exon 2 in the CDKN2B/p15 gene, not confirmed by DNA sequencing. Homozygous deletions and mutations are not involved in the inactivation mechanism of the CDKN2B/p15 and CDKN2A/p16 genes in neuroblastoma; however, these two abnormalities do not exclude other inactivation pathways. Recent evidence has shown that the expression of these genes is altered in this disease. Therefore, other mechanisms of inactivation, such as methylation of promoter region and unproperly function of proteins, may be considered in order to estimate the real contribution of these genes to neuroblastoma genesis or disease progression.

  13. Celastrol ameliorates HIV-1 Tat-induced inflammatory responses via NF-kappaB and AP-1 inhibition and heme oxygenase-1 induction in astrocytes

    Energy Technology Data Exchange (ETDEWEB)

    Youn, Gi Soo; Kwon, Dong-Joo; Ju, Sung Mi [Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University, Chunchon 200-702 (Korea, Republic of); Rhim, Hyangshuk [Department of Biomedical Sciences, Department of Medical Life Sciences, College of Medicine, the Catholic University of Korea, Seoul 137-701 (Korea, Republic of); Bae, Yong Soo [Department of Biological Science, College of Natural Sciences, Sungkyunkwan University, Suwon 440-746 (Korea, Republic of); Choi, Soo Young [Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University, Chunchon 200-702 (Korea, Republic of); Park, Jinseu, E-mail: jinpark@hallym.ac.kr [Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University, Chunchon 200-702 (Korea, Republic of)

    2014-10-01

    HIV-1 Tat causes extensive neuroinflammation that may progress to AIDS-related encephalitis and dementia. Celastrol possesses various biological activities such as anti-oxidant, anti-tumor, and anti-inflammatory activities. In this study, we investigated the modulatory effects of celastrol on HIV-1 Tat-induced inflammatory responses and the molecular mechanisms underlying its action in astrocytes. Pre-treatment of CRT-MG human astroglioma cells with celastrol significantly inhibited HIV-1 Tat-induced expression of ICAM-1/VCAM-1 and subsequent monocyte adhesiveness in CRT-MG cells. In addition, celastrol suppressed HIV-1 Tat-induced expression of pro-inflammatory chemokines, such as CXCL10, IL-8, and MCP-1. Celastrol decreased HIV-1 Tat-induced activation of JNK MAPK, AP-1, and NF-κB. Furthermore, celastrol induced mRNA and protein expression of HO-1 as well as Nrf2 activation. Blockage of HO-1 expression using siRNA reversed the inhibitory effect of celastrol on HIV-1 Tat-induced inflammatory responses. These results suggest that celastrol has regulatory effects on HIV-1 Tat-induced inflammatory responses by blocking the JNK MAPK-AP-1/NF-κB signaling pathways and inducing HO-1 expression in astrocytes. - Highlights: • Celastrol suppressed HIV-1 Tat-induced expression of pro-inflammatory genes. • Celastrol inhibited HIV-1 Tat -induced activation of JNK MAPK. • Celastrol inhibited HIV-1 Tat-induced activation of both NF-κB and AP-1. • Celastrol inhibited HIV-1 Tat-induced inflammatory responses via HO-1 induction.

  14. Molecular Weight-Dependent Immunostimulative Activity of Low Molecular Weight Chitosan via Regulating NF-κB and AP-1 Signaling Pathways in RAW264.7 Macrophages

    Science.gov (United States)

    Zheng, Bin; Wen, Zheng-Shun; Huang, Yun-Juan; Xia, Mei-Sheng; Xiang, Xing-Wei; Qu, You-Le

    2016-01-01

    Chitosan and its derivatives such as low molecular weight chitosans (LMWCs) have been found to possess many important biological properties, such as antioxidant and antitumor effects. In our previous study, LMWCs were found to elicit a strong immunomodulatory response in macrophages dependent on molecular weight. Herein we further investigated the molecular weight-dependent immunostimulative activity of LMWCs and elucidated its mechanism of action on RAW264.7 macrophages. LMWCs (3 kDa and 50 kDa of molecular weight) could significantly enhance the mRNA expression levels of COX-2, IL-10 and MCP-1 in a molecular weight and concentration-dependent manner. The results suggested that LMWCs elicited a significant immunomodulatory response, which was dependent on the dose and the molecular weight. Regarding the possible molecular mechanism of action, LMWCs promoted the expression of the genes of key molecules in NF-κB and AP-1 pathways, including IKKβ, TRAF6 and JNK1, and induced the phosphorylation of protein IKBα in RAW264.7 macrophage. Moreover, LMWCs increased nuclear translocation of p65 and activation of activator protein-1 (AP-1, C-Jun and C-Fos) in a molecular weight-dependent manner. Taken together, our findings suggested that LMWCs exert immunostimulative activity via activation of NF-κB and AP-1 pathways in RAW264.7 macrophages in a molecular weight-dependent manner and that 3 kDa LMWC shows great potential as a novel agent for the treatment of immune suppression diseases and in future vaccines. PMID:27657093

  15. AP1000{sup R} design robustness against extreme external events - Seismic, flooding, and aircraft crash

    Energy Technology Data Exchange (ETDEWEB)

    Pfister, A.; Goossen, C.; Coogler, K.; Gorgemans, J. [Westinghouse Electric Company LLC, 1000 Westinghouse Drive, Cranberry Township, PA 16066 (United States)

    2012-07-01

    Both the International Atomic Energy Agency (IAEA) and the U.S. Nuclear Regulatory Commission (NRC) require existing and new nuclear power plants to conduct plant assessments to demonstrate the unit's ability to withstand external hazards. The events that occurred at the Fukushima-Dai-ichi nuclear power station demonstrated the importance of designing a nuclear power plant with the ability to protect the plant against extreme external hazards. The innovative design of the AP1000{sup R} nuclear power plant provides unparalleled protection against catastrophic external events which can lead to extensive infrastructure damage and place the plant in an extended abnormal situation. The AP1000 plant is an 1100-MWe pressurized water reactor with passive safety features and extensive plant simplifications that enhance construction, operation, maintenance and safety. The plant's compact safety related footprint and protection provided by its robust nuclear island structures prevent significant damage to systems, structures, and components required to safely shutdown the plant and maintain core and spent fuel pool cooling and containment integrity following extreme external events. The AP1000 nuclear power plant has been extensively analyzed and reviewed to demonstrate that it's nuclear island design and plant layout provide protection against both design basis and extreme beyond design basis external hazards such as extreme seismic events, external flooding that exceeds the maximum probable flood limit, and malicious aircraft impact. The AP1000 nuclear power plant uses fail safe passive features to mitigate design basis accidents. The passive safety systems are designed to function without safety-grade support systems (such as AC power, component cooling water, service water, compressed air or HVAC). The plant has been designed to protect systems, structures, and components critical to placing the reactor in a safe shutdown condition within the steel

  16. An Insider's History of Some of the Significant Changes In the APS from the 1960s to Today

    Science.gov (United States)

    Schwartz, Brian

    2014-03-01

    It has been over 50 years since I first joined the American Physical Society. A lot has changed. The APS in the 60s did not have a single Forum and all divisions were related to sub-field of physics research plus the History of Physics Division. The APS governance and meeting's structure did not provide for issues relate to physics and society, undergraduate and graduate education, minorities, women, industrial physics, international physics, communication of science, and many more subjects quite typical of APS in 2014. From the start, I was a very active member of APS and one of the original petitioners for the Division on Physics and Society. Ultimately this led to the APS Forum structure, the Panel on Public Affairs and many APS formal committees. Two of the major impetuses for change in the late 60s were the need to debate the role of science in the unpopular war in Vietnam and the overproduction and poor employment prospects for new Ph.D.s. I, and colleagues, (with little original encouragement from the APS leadership), arranged for the discussion on many new topics at the meeting and proposed changes within the governance of the Society. In the late 1980s I joined the APS and was involved in many changes over the next 20 years. ultimately as Associate Executive Officer and Director of the APS Centennial in 1999. As indicated earlier, I've been with the APS for over 50 years and will present its history as a participant-insider. Topic covered include my surprise election to the APS governance in 1972, the establishment of the Forum on Physics and Society (and the restrictions imposed on its governance), my role as the father (or mother) of the women's movement at APS, the complexities and politics of the move of APS headquarters from New York to College Park, the establishment of the tabloid APS News and many more subjects. Currently I am producing staged-readings of science-based plays at the March and April 2014 APS meetings for the Forum on History of

  17. AP1000核电厂氢气点火器功能分析%Analysis of AP1000 Hydrogen Igniter Performance

    Institute of Scientific and Technical Information of China (English)

    林千; 周全福

    2012-01-01

    A lumped parameter code was used to study the AP1000 hydrogen igniter system and demonstrate its performance. In the worst case defined in this paper, the maximum hydrogen release rate was up to 300 kg/min. The results show that, if no ignition is initiated, the hydrogen concentration in some compartment is very high, the gas is burnable and it may induce the deflagration to detonation transition (DDT). While igniters on, and the concentration is reduced below the lower flammable limits. These demonstrate that the igniter system can effectively reduce the risk of hydrogen in severe accident.%采用集总参数分析程序对AP1000核电厂安全壳内氢气点火系统功能进行了分析和验证.在定义的包络事故工况下,氢气最大瞬时释放速率达300 kg/min.计算表明:在无点火措施情况下,AP1000安全壳局部隔间的氢气浓度较高,隔间内的气体处于可燃状态,且接近爆燃向爆炸转变( DDT)状态;在实施点火措施情况下,氢气浓度得到有效控制,氢气点火系统能消除严重事故下氢气所引起的风险.

  18. A Comparison of Van Allen Belt Radiation Environment Modeling Programs: AE8/AP8 Legacy, AE9/AP9, and SPENVIS

    Science.gov (United States)

    Reed, Evan; Pellish, Jonathan

    2016-01-01

    In the space surrounding Earth there exists an active radiation environment consisting mostly of electrons and protons that have been trapped by Earths magnetic field. This radiation, also known as the Van Allen Belts, has the potential to damage man-made satellites in orbit; thus, proper precautions must be taken to shield NASA assets from this phenomenon. Data on the Van Allen Belts has been collected continuously by a multitude of space-based instruments since the beginning of space exploration. Subsequently, using theory to fill in the gaps in the collected data, computer models have been developed that take in the orbital information of a hypothetical mission and output the expected particle fluence and flux for that orbit. However, as new versions of the modeling system are released, users are left wondering how the new version differs from the old. Therefore, we performed a comparison of three different editions of the modeling system: AE8/AP8 (legacy), which is included in the model 9 graphical user interface as an option for ones calculations, AE9/AP9, and the Space Environment Information System (SPENVIS), which is an online-based form of AE8/AP8 developed by NASA and the European Space Agency that changed the code to allow the program to extrapolate data to predict fluence and flux at higher energies. Although this evaluation is still ongoing, it is predicted that the model 8 (legacy) and SPENVIS version will have identical outputs with the exception of the extended energy levels from SPENVIS, while model 9 will provide different fluences than model 8 based on additional magnetic field descriptions and on-orbit data.

  19. Biological warfare agents.

    Science.gov (United States)

    Pohanka, Miroslav; Kuca, Kamil

    2010-01-01

    Biological warfare agents are a group of pathogens and toxins of biological origin that can be potentially misused for military or criminal purposes. The present review attempts to summarize necessary knowledge about biological warfare agents. The historical aspects, examples of applications of these agents such as anthrax letters, biological weapons impact, a summary of biological warfare agents and epidemiology of infections are described. The last section tries to estimate future trends in research on biological warfare agents.

  20. Space Environment Data Acquisition Equipment -Attached Payload (SEDA-AP) on the ISS -"Kibo" Exposed Facility

    Science.gov (United States)

    Koga, Kiyokazu; Matsumoto, Haruhisa; Kimoto, Yugo; Obara, Takahiro; Goka, Tateo

    To support future space activities, it is very important to acquire space environmental data related to space radiation degradation of space parts and materials and spacecraft anomalies. Such data are useful for spacecraft design and manned space activity. On several satellite of the Japan Aerospace Exploration Agency (JAXA) since the Engineering Test Satellite-V (ETS-V), Technical Data Acquisition Equipment (TEDA) and Space Environment Data Acquisition Equipment (SEDA) have been installed for obtaining the data described above. SEDA-Attached Payload (AP) was mounted on Japanese experimental module, "Kibo" , at International Space Station (ISS) to take continuous measurements of the 400 kilometres altitude space station's tra-jectory for a period of around 3 years. SEDA-AP comprises common bus equipment supporting launch, RMS handling, the power/communication interface with JEM-EF, an extendible mast that extends the neutron monitor sensor 1 m separate from the bus structure, and equipment that measures space environment data. SEDA-AP has been fitted with 8 kinds of instruments. It will continuously and simultaneously measure neutrons, heavy ions, plasma, high-energy electrons and protons, atomic oxygen, space debris and dusts, etc. Furthermore, by exposing electronic devices and materials directory to the space environment, it will examine how they are affected by the environment. SEDA-AP was lanced on July 16 in 2009, and attached to EF of "Kibo" on July 25 using the robot arm of "Kibo". Initial checkout was started on Au-gust 4 and successfully ended on September 17. This paper will report the mission objectives, instrumentation, and current status of SEDA-AP.